LOCUS       DAZFDC010000001       113965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFDC010000001 DAZFDC010000000
VERSION     DAZFDC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 113965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 113965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.001"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..414
                     /locus_tag="ACHJ48_000001"
     CDS             <1..414
                     /locus_tag="ACHJ48_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4705643.1"
                     /translation="GDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIK
                     NSLQMRIDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPS
                     GHGTVNMRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(457..1317)
                     /gene="rsmI"
                     /locus_tag="ACHJ48_000002"
     CDS             complement(457..1317)
                     /gene="rsmI"
                     /locus_tag="ACHJ48_000002"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO4705644.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1382..3418
                     /gene="lpoA"
                     /locus_tag="ACHJ48_000003"
     CDS             1382..3418
                     /gene="lpoA"
                     /locus_tag="ACHJ48_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO4705645.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFVGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3376..3771
                     /gene="yraN"
                     /locus_tag="ACHJ48_000004"
     CDS             3376..3771
                     /gene="yraN"
                     /locus_tag="ACHJ48_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO4705646.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDTFNDHS"
     gene            3791..4381
                     /gene="diaA"
                     /locus_tag="ACHJ48_000005"
     CDS             3791..4381
                     /gene="diaA"
                     /locus_tag="ACHJ48_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO4705647.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4391..4966
                     /gene="dolP"
                     /locus_tag="ACHJ48_000006"
     CDS             4391..4966
                     /gene="dolP"
                     /locus_tag="ACHJ48_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO4705648.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5080..6120)
                     /gene="yraQ"
                     /locus_tag="ACHJ48_000007"
     CDS             complement(5080..6120)
                     /gene="yraQ"
                     /locus_tag="ACHJ48_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO4705649.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6193..6828)
                     /gene="yraR"
                     /locus_tag="ACHJ48_000008"
     CDS             complement(6193..6828)
                     /gene="yraR"
                     /locus_tag="ACHJ48_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4705650.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6956..7474
                     /gene="yhbO"
                     /locus_tag="ACHJ48_000009"
     CDS             6956..7474
                     /gene="yhbO"
                     /locus_tag="ACHJ48_000009"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO4705651.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7454..7897)
                     /gene="yhbP"
                     /locus_tag="ACHJ48_000010"
     CDS             complement(7454..7897)
                     /gene="yhbP"
                     /locus_tag="ACHJ48_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO4705652.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRPDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            7948..8250
                     /gene="yhbQ"
                     /locus_tag="ACHJ48_000011"
     CDS             7948..8250
                     /gene="yhbQ"
                     /locus_tag="ACHJ48_000011"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO4705653.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAKLLSSLQTPEIKSD"
     gene            complement(8237..8740)
                     /gene="yhbS"
                     /locus_tag="ACHJ48_000012"
     CDS             complement(8237..8740)
                     /gene="yhbS"
                     /locus_tag="ACHJ48_000012"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4705654.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8734..9258)
                     /gene="ubiT"
                     /locus_tag="ACHJ48_000013"
     CDS             complement(8734..9258)
                     /gene="ubiT"
                     /locus_tag="ACHJ48_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO4705655.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9467..10462
                     /gene="ubiU"
                     /locus_tag="ACHJ48_000014"
     CDS             9467..10462
                     /gene="ubiU"
                     /locus_tag="ACHJ48_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO4705656.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10471..11349
                     /gene="ubiV"
                     /locus_tag="ACHJ48_000015"
     CDS             10471..11349
                     /gene="ubiV"
                     /locus_tag="ACHJ48_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO4705657.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11430..12437
                     /gene="yhbW"
                     /locus_tag="ACHJ48_000016"
     CDS             11430..12437
                     /gene="yhbW"
                     /locus_tag="ACHJ48_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFO4705658.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSARDAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVAWFDARDPNPHVRPVPG
                     YGEQIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12554..13798)
                     /gene="mtr"
                     /locus_tag="ACHJ48_000017"
     CDS             complement(12554..13798)
                     /gene="mtr"
                     /locus_tag="ACHJ48_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO4705659.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIDIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13952..15841)
                     /gene="deaD"
                     /locus_tag="ACHJ48_000018"
     CDS             complement(13952..15841)
                     /gene="deaD"
                     /locus_tag="ACHJ48_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO4705660.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15834..15914)
                     /gene="yrbN"
                     /locus_tag="ACHJ48_000019"
     CDS             complement(15834..15914)
                     /gene="yrbN"
                     /locus_tag="ACHJ48_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO4705661.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16021..16905)
                     /gene="nlpI"
                     /locus_tag="ACHJ48_000020"
     CDS             complement(16021..16905)
                     /gene="nlpI"
                     /locus_tag="ACHJ48_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO4705662.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17014..19149)
                     /gene="pnp"
                     /locus_tag="ACHJ48_000021"
     CDS             complement(17014..19149)
                     /gene="pnp"
                     /locus_tag="ACHJ48_000021"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO4705663.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19396..19665)
                     /gene="rpsO"
                     /locus_tag="ACHJ48_000022"
     CDS             complement(19396..19665)
                     /gene="rpsO"
                     /locus_tag="ACHJ48_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO4705664.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR"
     gene            complement(19814..20758)
                     /gene="truB"
                     /locus_tag="ACHJ48_000023"
     CDS             complement(19814..20758)
                     /gene="truB"
                     /locus_tag="ACHJ48_000023"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO4705665.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20758..21159)
                     /gene="rbfA"
                     /locus_tag="ACHJ48_000024"
     CDS             complement(20758..21159)
                     /gene="rbfA"
                     /locus_tag="ACHJ48_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO4705666.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21324..23996)
                     /gene="infB"
                     /locus_tag="ACHJ48_000025"
     CDS             complement(21324..23996)
                     /gene="infB"
                     /locus_tag="ACHJ48_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO4705667.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24021..25508)
                     /gene="nusA"
                     /locus_tag="ACHJ48_000026"
     CDS             complement(24021..25508)
                     /gene="nusA"
                     /locus_tag="ACHJ48_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO4705668.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25536..25988)
                     /gene="rimP"
                     /locus_tag="ACHJ48_000027"
     CDS             complement(25536..25988)
                     /gene="rimP"
                     /locus_tag="ACHJ48_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO4705669.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26195..26271)
                     /locus_tag="ACHJ48_000028"
     tRNA            complement(26195..26271)
                     /locus_tag="ACHJ48_000028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26235..26237),aa:Met,seq:cat)
     gene            26619..27962
                     /gene="argG"
                     /locus_tag="ACHJ48_000029"
     CDS             26619..27962
                     /gene="argG"
                     /locus_tag="ACHJ48_000029"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO4705670.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(27970..29595)
                     /gene="yhbX"
                     /locus_tag="ACHJ48_000030"
     CDS             complement(27970..29595)
                     /gene="yhbX"
                     /locus_tag="ACHJ48_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4705671.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVAKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIVHYKGDS
                     LVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(30054..30140)
                     /locus_tag="ACHJ48_000031"
     tRNA            complement(30054..30140)
                     /locus_tag="ACHJ48_000031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30104..30106),aa:Leu,seq:gag)
     gene            complement(30155..30487)
                     /gene="secG"
                     /locus_tag="ACHJ48_000032"
     CDS             complement(30155..30487)
                     /gene="secG"
                     /locus_tag="ACHJ48_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO4705672.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30715..32052)
                     /gene="glmM"
                     /locus_tag="ACHJ48_000033"
     CDS             complement(30715..32052)
                     /gene="glmM"
                     /locus_tag="ACHJ48_000033"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO4705673.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVETALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32045..32893)
                     /gene="folP"
                     /locus_tag="ACHJ48_000034"
     CDS             complement(32045..32893)
                     /gene="folP"
                     /locus_tag="ACHJ48_000034"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO4705674.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32983..34917)
                     /gene="ftsH"
                     /locus_tag="ACHJ48_000035"
     CDS             complement(32983..34917)
                     /gene="ftsH"
                     /locus_tag="ACHJ48_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO4705675.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNAGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35017..35646)
                     /gene="rlmE"
                     /locus_tag="ACHJ48_000036"
     CDS             complement(35017..35646)
                     /gene="rlmE"
                     /locus_tag="ACHJ48_000036"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO4705676.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35772..36065
                     /gene="yhbY"
                     /locus_tag="ACHJ48_000037"
     CDS             35772..36065
                     /gene="yhbY"
                     /locus_tag="ACHJ48_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO4705677.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36221..36697)
                     /gene="greA"
                     /locus_tag="ACHJ48_000038"
     CDS             complement(36221..36697)
                     /gene="greA"
                     /locus_tag="ACHJ48_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO4705678.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36945..38378
                     /gene="dacB"
                     /locus_tag="ACHJ48_000039"
     CDS             36945..38378
                     /gene="dacB"
                     /locus_tag="ACHJ48_000039"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4705679.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAVAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38418..39590)
                     /gene="cgtA"
                     /locus_tag="ACHJ48_000040"
     CDS             complement(38418..39590)
                     /gene="cgtA"
                     /locus_tag="ACHJ48_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO4705680.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39606..40571)
                     /gene="yhbE"
                     /locus_tag="ACHJ48_000041"
     CDS             complement(39606..40571)
                     /gene="yhbE"
                     /locus_tag="ACHJ48_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4705681.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40698..40955)
                     /gene="rpmA"
                     /locus_tag="ACHJ48_000042"
     CDS             complement(40698..40955)
                     /gene="rpmA"
                     /locus_tag="ACHJ48_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO4705682.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40976..41287)
                     /gene="rplU"
                     /locus_tag="ACHJ48_000043"
     CDS             complement(40976..41287)
                     /gene="rplU"
                     /locus_tag="ACHJ48_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO4705683.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41546..42517
                     /gene="ispB"
                     /locus_tag="ACHJ48_000044"
     CDS             41546..42517
                     /gene="ispB"
                     /locus_tag="ACHJ48_000044"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO4705684.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLKPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42746..43024
                     /gene="sfsB"
                     /locus_tag="ACHJ48_000045"
     CDS             42746..43024
                     /gene="sfsB"
                     /locus_tag="ACHJ48_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO4705685.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43072..44331)
                     /gene="murA"
                     /locus_tag="ACHJ48_000046"
     CDS             complement(43072..44331)
                     /gene="murA"
                     /locus_tag="ACHJ48_000046"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO4705686.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNTQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44386..44640)
                     /gene="ibaG"
                     /locus_tag="ACHJ48_000047"
     CDS             complement(44386..44640)
                     /gene="ibaG"
                     /locus_tag="ACHJ48_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO4705687.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44800..45093)
                     /gene="mlaB"
                     /locus_tag="ACHJ48_000048"
     CDS             complement(44800..45093)
                     /gene="mlaB"
                     /locus_tag="ACHJ48_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO4705688.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45093..45728)
                     /gene="mlaC"
                     /locus_tag="ACHJ48_000049"
     CDS             complement(45093..45728)
                     /gene="mlaC"
                     /locus_tag="ACHJ48_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO4705689.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45747..46298)
                     /gene="mlaD"
                     /locus_tag="ACHJ48_000050"
     CDS             complement(45747..46298)
                     /gene="mlaD"
                     /locus_tag="ACHJ48_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO4705690.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46303..47085)
                     /gene="mlaE"
                     /locus_tag="ACHJ48_000051"
     CDS             complement(46303..47085)
                     /gene="mlaE"
                     /locus_tag="ACHJ48_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO4705691.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47093..47902)
                     /gene="mlaF"
                     /locus_tag="ACHJ48_000052"
     CDS             complement(47093..47902)
                     /gene="mlaF"
                     /locus_tag="ACHJ48_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO4705692.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48112..49089
                     /gene="yrbG"
                     /locus_tag="ACHJ48_000053"
     CDS             48112..49089
                     /gene="yrbG"
                     /locus_tag="ACHJ48_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO4705693.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49103..50089
                     /gene="kdsD"
                     /locus_tag="ACHJ48_000054"
     CDS             49103..50089
                     /gene="kdsD"
                     /locus_tag="ACHJ48_000054"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO4705694.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHIPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50110..50676
                     /gene="kdsC"
                     /locus_tag="ACHJ48_000055"
     CDS             50110..50676
                     /gene="kdsC"
                     /locus_tag="ACHJ48_000055"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO4705695.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50673..51248
                     /gene="lptC"
                     /locus_tag="ACHJ48_000056"
     CDS             50673..51248
                     /gene="lptC"
                     /locus_tag="ACHJ48_000056"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO4705696.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51217..51774
                     /gene="lptA"
                     /locus_tag="ACHJ48_000057"
     CDS             51217..51774
                     /gene="lptA"
                     /locus_tag="ACHJ48_000057"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO4705697.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51781..52506
                     /gene="lptB"
                     /locus_tag="ACHJ48_000058"
     CDS             51781..52506
                     /gene="lptB"
                     /locus_tag="ACHJ48_000058"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO4705698.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52554..53987
                     /gene="rpoN"
                     /locus_tag="ACHJ48_000059"
     CDS             52554..53987
                     /gene="rpoN"
                     /locus_tag="ACHJ48_000059"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO4705699.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54010..54297
                     /gene="hpf"
                     /locus_tag="ACHJ48_000060"
     CDS             54010..54297
                     /gene="hpf"
                     /locus_tag="ACHJ48_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO4705700.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54415..54906
                     /gene="ptsN"
                     /locus_tag="ACHJ48_000061"
     CDS             54415..54906
                     /gene="ptsN"
                     /locus_tag="ACHJ48_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO4705701.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTKGTP
                     DEA"
     gene            54952..55806
                     /gene="rapZ"
                     /locus_tag="ACHJ48_000062"
     CDS             54952..55806
                     /gene="rapZ"
                     /locus_tag="ACHJ48_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO4705702.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55803..56075
                     /gene="npr"
                     /locus_tag="ACHJ48_000063"
     CDS             55803..56075
                     /gene="npr"
                     /locus_tag="ACHJ48_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO4705703.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56289..56921
                     /gene="yrbL"
                     /locus_tag="ACHJ48_000064"
     CDS             56289..56921
                     /gene="yrbL"
                     /locus_tag="ACHJ48_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO4705704.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(56918..57646)
                     /gene="mtgA"
                     /locus_tag="ACHJ48_000065"
     CDS             complement(56918..57646)
                     /gene="mtgA"
                     /locus_tag="ACHJ48_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO4705705.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDRVSMDQISPWMGLAVIAAEDQTFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57643..58296)
                     /gene="elbB"
                     /locus_tag="ACHJ48_000066"
     CDS             complement(57643..58296)
                     /gene="elbB"
                     /locus_tag="ACHJ48_000066"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO4705706.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58526..60862)
                     /gene="arcB"
                     /locus_tag="ACHJ48_000067"
     CDS             complement(58526..60862)
                     /gene="arcB"
                     /locus_tag="ACHJ48_000067"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO4705707.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVNVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60958..61887)
                     /gene="yhcC"
                     /locus_tag="ACHJ48_000068"
     CDS             complement(60958..61887)
                     /gene="yhcC"
                     /locus_tag="ACHJ48_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO4705708.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62561..67021
                     /gene="gltB"
                     /locus_tag="ACHJ48_000069"
     CDS             62561..67021
                     /gene="gltB"
                     /locus_tag="ACHJ48_000069"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO4705709.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNEI
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHNDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTET
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67034..68452
                     /gene="gltD"
                     /locus_tag="ACHJ48_000070"
     CDS             67034..68452
                     /gene="gltD"
                     /locus_tag="ACHJ48_000070"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO4705710.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68636..69685
                     /gene="yhcG"
                     /locus_tag="ACHJ48_000071"
     CDS             68636..69685
                     /gene="yhcG"
                     /locus_tag="ACHJ48_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO4705711.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLPWSTYVRLLSVKNADTRSFYEKETLRCGWSVRQLERQIATQFYERTL
                     LSHDKSAMLQQHAPAETHILPQQAIRDPFVLEFLELKDEYSESDFEEALINHLMDFML
                     ELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLLIVDLKVGKFSYSDAGQMNMYL
                     NYAKEHWTLPDENPPIGLVLCAEKGVGEAHYALAGLPNTVLASEYKMQLPDEKRLADE
                     LVRTQAVLEEGYRLR"
     gene            complement(69745..70209)
                     /gene="nanQ"
                     /locus_tag="ACHJ48_000072"
     CDS             complement(69745..70209)
                     /gene="nanQ"
                     /locus_tag="ACHJ48_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO4705712.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(70206..71081)
                     /gene="nanK"
                     /locus_tag="ACHJ48_000073"
     CDS             complement(70206..71081)
                     /gene="nanK"
                     /locus_tag="ACHJ48_000073"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO4705713.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(71078..71767)
                     /gene="nanE"
                     /locus_tag="ACHJ48_000074"
     CDS             complement(71078..71767)
                     /gene="nanE"
                     /locus_tag="ACHJ48_000074"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO4705714.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(71815..73305)
                     /gene="nanT"
                     /locus_tag="ACHJ48_000075"
     CDS             complement(71815..73305)
                     /gene="nanT"
                     /locus_tag="ACHJ48_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO4705715.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(73414..74307)
                     /gene="nanA"
                     /locus_tag="ACHJ48_000076"
     CDS             complement(73414..74307)
                     /gene="nanA"
                     /locus_tag="ACHJ48_000076"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO4705716.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(74429..75220)
                     /gene="nanR"
                     /locus_tag="ACHJ48_000077"
     CDS             complement(74429..75220)
                     /gene="nanR"
                     /locus_tag="ACHJ48_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO4705717.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            75601..76957
                     /gene="dcuC"
                     /locus_tag="ACHJ48_000078"
                     /pseudo
     CDS             75601..76957
                     /gene="dcuC"
                     /locus_tag="ACHJ48_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(77000..77497)
                     /gene="sspB"
                     /locus_tag="ACHJ48_000079"
     CDS             complement(77000..77497)
                     /gene="sspB"
                     /locus_tag="ACHJ48_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO4705718.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77503..78141)
                     /gene="sspA"
                     /locus_tag="ACHJ48_000080"
     CDS             complement(77503..78141)
                     /gene="sspA"
                     /locus_tag="ACHJ48_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO4705719.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78536..78928)
                     /gene="rpsI"
                     /locus_tag="ACHJ48_000081"
     CDS             complement(78536..78928)
                     /gene="rpsI"
                     /locus_tag="ACHJ48_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO4705720.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78944..79372)
                     /gene="rplM"
                     /locus_tag="ACHJ48_000082"
     CDS             complement(78944..79372)
                     /gene="rplM"
                     /locus_tag="ACHJ48_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO4705721.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79591..80718)
                     /gene="zapE"
                     /locus_tag="ACHJ48_000083"
     CDS             complement(79591..80718)
                     /gene="zapE"
                     /locus_tag="ACHJ48_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO4705722.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80912..81310
                     /gene="zapG"
                     /locus_tag="ACHJ48_000084"
     CDS             80912..81310
                     /gene="zapG"
                     /locus_tag="ACHJ48_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO4705723.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81464..82831
                     /gene="degQ"
                     /locus_tag="ACHJ48_000085"
     CDS             81464..82831
                     /gene="degQ"
                     /locus_tag="ACHJ48_000085"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO4705724.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKTGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            82921..83988
                     /gene="degS"
                     /locus_tag="ACHJ48_000086"
     CDS             82921..83988
                     /gene="degS"
                     /locus_tag="ACHJ48_000086"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO4705725.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(84052..84990)
                     /gene="mdh"
                     /locus_tag="ACHJ48_000087"
     CDS             complement(84052..84990)
                     /gene="mdh"
                     /locus_tag="ACHJ48_000087"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4705726.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85425..85895
                     /gene="argR"
                     /locus_tag="ACHJ48_000088"
     CDS             85425..85895
                     /gene="argR"
                     /locus_tag="ACHJ48_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO4705727.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            86260..86523
                     /gene="yhcN"
                     /locus_tag="ACHJ48_000089"
     CDS             86260..86523
                     /gene="yhcN"
                     /locus_tag="ACHJ48_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO4705728.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(86579..86851)
                     /gene="yhcO"
                     /locus_tag="ACHJ48_000090"
     CDS             complement(86579..86851)
                     /gene="yhcO"
                     /locus_tag="ACHJ48_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO4705729.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(86943..88910)
                     /gene="aaeB"
                     /locus_tag="ACHJ48_000091"
     CDS             complement(86943..88910)
                     /gene="aaeB"
                     /locus_tag="ACHJ48_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO4705730.1"
                     /translation="MDIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LCIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(88916..89845)
                     /gene="aaeA"
                     /locus_tag="ACHJ48_000092"
     CDS             complement(88916..89845)
                     /gene="aaeA"
                     /locus_tag="ACHJ48_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO4705731.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQE
                     KRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAPA
                     DGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGSN
                     KVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWPA
                     GTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(89853..90056)
                     /gene="aaeX"
                     /locus_tag="ACHJ48_000093"
     CDS             complement(89853..90056)
                     /gene="aaeX"
                     /locus_tag="ACHJ48_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO4705732.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVCRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            90239..91168
                     /gene="aaeR"
                     /locus_tag="ACHJ48_000094"
     CDS             90239..91168
                     /gene="aaeR"
                     /locus_tag="ACHJ48_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO4705733.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(91302..92747)
                     /gene="tldD"
                     /locus_tag="ACHJ48_000095"
     CDS             complement(91302..92747)
                     /gene="tldD"
                     /locus_tag="ACHJ48_000095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO4705734.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFVILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIARD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLIEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(92903..96703)
                     /gene="yhdP"
                     /locus_tag="ACHJ48_000096"
     CDS             complement(92903..96703)
                     /gene="yhdP"
                     /locus_tag="ACHJ48_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO4705735.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLGIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWSDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(96771..98240)
                     /gene="rng"
                     /locus_tag="ACHJ48_000097"
     CDS             complement(96771..98240)
                     /gene="rng"
                     /locus_tag="ACHJ48_000097"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO4705736.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(98230..98823)
                     /gene="yhdE"
                     /locus_tag="ACHJ48_000098"
     CDS             complement(98230..98823)
                     /gene="yhdE"
                     /locus_tag="ACHJ48_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO4705737.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSRHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYQAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(98832..99320)
                     /gene="mreD"
                     /locus_tag="ACHJ48_000099"
     CDS             complement(98832..99320)
                     /gene="mreD"
                     /locus_tag="ACHJ48_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO4705738.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(99320..100423)
                     /gene="mreC"
                     /locus_tag="ACHJ48_000100"
     CDS             complement(99320..100423)
                     /gene="mreC"
                     /locus_tag="ACHJ48_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO4705739.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSLQRATPPQSGAQPPARAPGGQ"
     gene            complement(100489..101532)
                     /gene="mreB"
                     /locus_tag="ACHJ48_000101"
     CDS             complement(100489..101532)
                     /gene="mreB"
                     /locus_tag="ACHJ48_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO4705740.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(101837..103777)
                     /gene="csrD"
                     /locus_tag="ACHJ48_000102"
     CDS             complement(101837..103777)
                     /gene="csrD"
                     /locus_tag="ACHJ48_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO4705741.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            103929..104903
                     /gene="yhdH"
                     /locus_tag="ACHJ48_000103"
     CDS             103929..104903
                     /gene="yhdH"
                     /locus_tag="ACHJ48_000103"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO4705742.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            105881..106351
                     /gene="accB"
                     /locus_tag="ACHJ48_000104"
     CDS             105881..106351
                     /gene="accB"
                     /locus_tag="ACHJ48_000104"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO4705743.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            106362..107711
                     /gene="accC"
                     /locus_tag="ACHJ48_000105"
     CDS             106362..107711
                     /gene="accC"
                     /locus_tag="ACHJ48_000105"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO4705744.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNSIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            107820..108062
                     /gene="yhdT"
                     /locus_tag="ACHJ48_000106"
     CDS             107820..108062
                     /gene="yhdT"
                     /locus_tag="ACHJ48_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO4705745.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            108052..109503
                     /gene="panF"
                     /locus_tag="ACHJ48_000107"
     CDS             108052..109503
                     /gene="panF"
                     /locus_tag="ACHJ48_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO4705746.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            109515..110396
                     /gene="prmA"
                     /locus_tag="ACHJ48_000108"
     CDS             109515..110396
                     /gene="prmA"
                     /locus_tag="ACHJ48_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO4705747.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            110725..111690
                     /gene="dusB"
                     /locus_tag="ACHJ48_000109"
     CDS             110725..111690
                     /gene="dusB"
                     /locus_tag="ACHJ48_000109"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO4705748.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            111716..112012
                     /gene="fis"
                     /locus_tag="ACHJ48_000110"
     CDS             111716..112012
                     /gene="fis"
                     /locus_tag="ACHJ48_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO4705749.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            112097..112981
                     /gene="yhdJ"
                     /locus_tag="ACHJ48_000111"
     CDS             112097..112981
                     /gene="yhdJ"
                     /locus_tag="ACHJ48_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4705750.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            113065..113244
                     /gene="yhdU"
                     /locus_tag="ACHJ48_000112"
     CDS             113065..113244
                     /gene="yhdU"
                     /locus_tag="ACHJ48_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO4705751.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(113247..113909)
                     /gene="acrS"
                     /locus_tag="ACHJ48_000113"
     CDS             complement(113247..113909)
                     /gene="acrS"
                     /locus_tag="ACHJ48_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO4705752.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT        27865 a        30659 c        28408 g        27033 t
ORIGIN      
        1 ggcgatgtta ttaaggttaa ttttggattc atcaatggtc agaagtttac cacccgcaat
       61 gccatgccag acggttttac tccagtagac tttgatatca cttatgactg tggtgatact
      121 tcaaagatta aaaactcgtt gcaaatgcgc atcgacggta caactggggt agtagaccag
      181 tacaacctgg tcgccagacg aagaagttca gacaatgcgc ccgatgtcgg tattcgtatt
      241 gaaaatctcg gcggcggagt tgcaaatatt ccttttcaga acggtatcct tcccgttgat
      301 ccttccgggc atggcaccgt caatatgcgc gcctggccag ttaatctggt cggtggtgag
      361 ctggaaacag gaaaatttca gggcacagcc accattaccg tcatcgtgcg gtaaaagaag
      421 acgttgcgtt ttgattaacg caacgcttcg gcactcttac ccctgctgct ccagcgcata
      481 cttatacagc gcatttttct tcacgccgtg aatttctgcg gccagcgccg ccgctttttt
      541 cagcggcagt tctgcctgta gcagcgccag cgtgcgcagg gcatcggcgg gtaagtcttc
      601 ttcctgtgct ttatgacctt cgacaatcag caccatttcg cctttgcgac ggttttcatc
      661 ttcctttacc cacgccagca gctcgccaac gggcgcgccg tgaatggttt cccaggtttt
      721 ggtcagctca cgcgccagaa ccacgtagcg ggattcgcct aataccgcaa cgatatcttc
      781 caggctatct aacagacggt gggtagattc ataaaaaatc agcgtgcgcg gctccgcttc
      841 aatggctttt agcgcatcac ggcggccttt tgatttggca ggtaaaaagc cttcgtaaca
      901 gaaacggtca gagggtaaac ccgcggcgct taacgcagtg atagcagcac acggtccggg
      961 tagcggcacc acacgaatcc ccgcttcacg gcaggtacgc accagatggt agccaggatc
     1021 gttaattagc ggcgttccgg catcggaaac cagcgcaatg ttttgcccct cttgcagctt
     1081 cgccagcagc gtttcggctt tttgttgttc gttatggtcg tgcagcgcaa acaaccgggc
     1141 attaatccca aaatgttgca gcaataaacc ggtgtgacga gtatcctcgg cggcaatcag
     1201 atcaacggcc tgtaatacct ctaacgcacg ctgggtgata tccgccagat tgccgattgg
     1261 cgtcggtaca atgtaaagct ggccctgaga attatccgcc gattggtgtt gtttcattgt
     1321 gtcgtccgta ttgccgattt aatattgagc attgcgtaaa aaaaatatca ctggatacat
     1381 tatggtaccc tcaacatttt ctcgtttgaa agccgcgcgt tgtctgcctg ttgttctggc
     1441 agccctgatt ttcgtcggtt gtggcaccca tactcccgat cagtccactg cttatatgca
     1501 gggcacggcg caggctgatt ctgcctttta tcttcagcag atgcagcaaa gctctgatga
     1561 taccaggatc aactggcaat tactcgccat tcgtgcactg gtgaaagaag gtaaaaccgg
     1621 gcaggcggtt gagttgttta accaactacc gcaagaactg aacgattctc agcgtcgcga
     1681 gaaaacactg ctggcggtag agattaaact ggcgcagaaa gattttgctg gcgcgcaaaa
     1741 cttgctggcg aaaattacac ccgccgattt agaacaaaac cagcaagccc gttactggca
     1801 ggcaaaaatc gatgccagcc aggggcgtcc ttccattgat ttactgcgcg cgttaattgc
     1861 tcaggaaccg ctgcttggcg cgaaagaaaa acagcagaat attgatgcca cctggcaggc
     1921 gctctcctcc atgactcagg aacaggcgaa tacgctggtg atcaacgccg acgaaaatat
     1981 tctgcaaggc tggctggatc tgcagcgcgt ctggtttgat aaccgtaacg atcccgacat
     2041 gatgaaagcc gggatcgccg actggcagaa acgttatccg aacaatccgg gcgcgaaaat
     2101 gctgccaacg cagttggtta acgtaaaagc gtttaaacca gcctcgacca acaaaatcgc
     2161 cctgctgttg ccactgaatg gccaggcagc ggtatttggt cgcactattc agcaaggctt
     2221 tgaagcggcg aaaaatatcg gcactcagcc agtggcggct caggtagctg ccgcacctgc
     2281 cgcagacgta gcagaacaac ctcagccgca aactgcggat ggcgttgcca gcccggcaca
     2341 agcctcggtt agcgatctga ccggtgatca gcctgcggcc cagccggtgc ctgtaagcgc
     2401 cccggcgaca agcaccgcag cggtaagcgc acccgcaaat ccatccgcag agctgaaaat
     2461 ctacgatacc tcatcacaac cacttagcca gatcttaagc caggttcagc aggatggcgc
     2521 gagtattgtg gttggtccgt tgctgaaaaa taacgttgaa gagttgctga agagcaacac
     2581 tccgctgaac gtactagcac tgaaccagcc ggagaatatc gaaaatcgcg tcaatatttg
     2641 ttacttcgcg ctttcaccgg aagacgaagc gcgcgatgca gcgcgtcata ttcgtgacca
     2701 gggtaaacaa gcgccgctgg tgctgatccc acgcagttca ttgggcgatc gcgtagccaa
     2761 tgcgtttgcg caagagtggc agaaattggg cggcggcacc gttctgcaac aaaaatttgg
     2821 ttccaccagc gaattacgcg cgggtgttaa cggcggttct ggtattgctt taacgggtac
     2881 cccgattact cccagagcga caaccgactc cggcatgacg accaacaatc caacgctgca
     2941 aaccacgcca accgatgacc agttcaccaa taatggcggt cgtgtcgatg cggtgtacat
     3001 tgtggcaacg ccgggtgaaa tcgcttttat taaaccgatg atcgccatgc gtaacggtag
     3061 ccagagcggt gcaacgctgt acgccagctc ccgcagtgcg caagggaccg ctggcccgga
     3121 tttccgtctg gagatggaag gcttgcagta cagcgaaatc ccgatgctgg cgggcggtaa
     3181 tctgccgtta atgcagcagg cactcagcgc ggtgaataac gattattcac tggctcgcat
     3241 gtatgcgatg ggcgtcgatg cctggtcgct ggcaaatcat ttctcacaaa tgcgccaggt
     3301 tcagggtttt gaaatcaacg gtaataccgg aagcctgacg gctaacccgg attgcgtgat
     3361 taacaggaag ttatcatggc tacagtacca acaaggtcag gtagtccccg ccagttaacc
     3421 accaaacaga ccggcgatgc gtgggaagta caagcgcgtc gctggctgga aggcaaagga
     3481 ctgcgatttg tcgccgctaa cgtgaacgag cgtggcggcg agatcgatct gataatgcgt
     3541 gaaggccgga ccaccgtttt tgtcgaggtg cgttaccgcc gttctgcgct ttatggcggc
     3601 gcggcagcca gtgtgacccg cagcaaacaa cacaaattat tacagactgc ccgcttgtgg
     3661 ctcgcgcgtc ataatgggag ttttgatact gtggattgcc ggttcgatgt ggtagccttc
     3721 accgggaatg aggttgagtg gattaaggat acctttaatg accactcata attaaggttt
     3781 aaggattagc gtgcaagaaa gaattaaagc ttgcttcact gaaagcattc aaactcaaat
     3841 tgcggcggca gaggcgcttc cggatgccat ctcccgtgcg gccatgacgc tggttcagtc
     3901 tctgctcaat ggcaacaaaa tcctctgttg tggtaatgga acttccgctg ccaatgcaca
     3961 gcattttgct gccagcatga tcaaccgttt cgaaacggag cggcccagct tacctgccat
     4021 tgcactaaat accgataatg ttgtcttaac ggcgattgcc aacgatcgct tacatgatga
     4081 agtgtatgca aaacaggtgc gggcgctggg gcatgcggga gatgtattgt tagccatttc
     4141 cacccgtggc aacagccgcg atatcgtaaa agcagtagaa gctgccgtta cgcgtgatat
     4201 gaccattgtg gcattgaccg gttatgacgg cggtgaactt gcaggtttgt tagggccaca
     4261 ggatgtggag atccgcatcc cttcgcatcg cagtgctcgc attcaggaaa tgcatatgct
     4321 gacggtaaat tgcctgtgcg atctgatcga taacacgctt ttccctcacc aggatgatta
     4381 aggagaatac atgaaggcat tatcgccaat cgcagtcctt atttccgcgc tgctgttgca
     4441 aggttgtgtt gccgctgccg tagtgggtac tgctgctgtg ggtaccaaag ccgcaactga
     4501 cccacgcagt gtcggcaccc aggtggacga tggtaccctg gaagtgcgcg tgaacagcgc
     4561 attgtcgaaa gacgaacaga ttaagaaaga agcgcgcatt aatgtaacgg cctatcaggg
     4621 caaagtgctg ctggttgggc agtcaccaaa cgctgaactt tcggctcgcg ccaaacagat
     4681 tgctatgggc gtagacggtg ccaacgaagt gtataacgag attcgtcagg gccagccgat
     4741 tggtctgggc gaagcgtcta acgatacgtg gatcaccacc aaagtgcgtt cgcagctctt
     4801 aaccagcgac ctggtgaaat cgtccaacgt gaaagtgacc accgaaaacg gtgaagtgtt
     4861 cctgatgggg ctggtgactg aacgtgaagc gaaagcggcg gcagatattg ccagccgggt
     4921 gagcggcgtg aagcgggtaa ctacggcgtt tacgtttatt aaatagcagc cccttgtaat
     4981 gcctgatgcg acgcttgccg cgtcttatca ggcctacaac gatgcaaacc gtaggtcgga
     5041 taaggcgttt acgccgcatc cgacatgttt tccctcaaat cagaataaca gcgccagccc
     5101 gccgacaatc acgcctgaca ctgccaccat cgcccccgtc agccataagg ctttcgccgg
     5161 gaacgctttg cgcagcataa tcagtgacgg caaactcacc gccgggagcg tcatcaacaa
     5221 tgccagcgcc ggagcagttc ccatacctgc cagcatcatc gtttgtacaa tcggaatttc
     5281 tgctgccgtg ggaatgacaa acaagcaccc tgctaccgcc atcgccacca cccacatcag
     5341 gctgttatcg acagcaccat cggcatgggg gaataaccag acgcgagcgg cacccaacac
     5401 cagtactgca aggatgtaaa ccgggatcgt actccagaaa agcgtccata gcgccctgcc
     5461 ccagcgacta aaaaatccgc cctgtgcttc cggtatgtca atttcgaccg gtgcttgcgt
     5521 ttgcggtgtt tcacgcaccc atttttgcac cagcgtcgca atcagcaaca ccatcaccag
     5581 cccggccacc agacgaatcg ccgcaaaacc ccagccgagg acaaacccca taaacaccag
     5641 cgtcgccggg tttaacaccg gattgcccat ccagaatgcc agcgcaccgc ccatcgacac
     5701 ctgttggcga cgcattcccg ccgcgaccgg agccgcacag caggtacaca tcatgcctgg
     5761 cagcgaaaac agcgttccca gcagcgtgcc gcgaaagcgc gattgcccaa gcgtacgcaa
     5821 caaccagtca cgcgggatca gcacctgaat caacgaaccg aggatcaccc ccagcaccgc
     5881 cgctttccat accgcgagga aatagatcat cgcgtaatcc aacgccgcct gccatggatt
     5941 agcatccgcc tgcgcaagga tagatttacc gatactgtgg gtttcggcag cagtaaacgc
     6001 tttgccgtag taaggttgcc atttcacata ccagagaccg gcaatgacaa cgagaaagaa
     6061 aagcgcgggt ttccaccact gaatgggcgt tgccgcctga gatgaagact gaccagtcat
     6121 agcattcccc aggaataagt tatgataatt gagcgcgtga atattacgct cactatcaat
     6181 tcttgggaat aattattcag ctcttttgcg taattctgaa gagcttaaaa tcgtcacacc
     6241 ttcatgctcg ggccgcatcg cctctgccag catgacccgc gccacgtctc tcgcatcaat
     6301 ggatttccag ttaccgggta acaaacggaa cagcggcgca aaaagcgttt cgttcatccg
     6361 ctgtttgcta cgatcgccca gtaacatcga cgggcgagca atggtcaatt tcggccagtt
     6421 ctgggcgatt aatgcttctt ccatctcccc tttgacgcgg ttatagaaaa acggcgagtg
     6481 ggcattggca cccatagcac tgaccaccag catatgctgc gcgcccagtc gccgcccggt
     6541 taatgcggta tccactacca gcgtgtaatc ggcatgaata aacgcctctt tgctccccgc
     6601 ttctcgccgc gtggtgccga gacaacaaaa cacaatgtcg atgggatcgg tgacctgcgc
     6661 cagcgcgtca gtcagttgcg gatcatgggg attaaatacc ccgggcatat cgcccaacgg
     6721 acgtcgcgtc ggcgcggcta tggcgttaac tttcggttcg ttaatcaaca tccgcagcag
     6781 gtgaccgccc accagccccg ttgcgcctgt aatcagtacc tgactcatct tcgctccttt
     6841 acagaattgt ctgccttgcg ctccacggct catgcaccag gcttaataga ccatgaggta
     6901 attatccccg attgtgggaa attcgcctca tccaatgcaa caacgcggag gaagcatgag
     6961 taagaaaatt gccgttttaa tcactgatga atttgaggat tcagaattta cttcacccgc
     7021 agacgagttc cgtaaagccg gacacgaagt gattaccatt gaaaaacaag cgggtaaaac
     7081 ggtgaaaggc aaaaaaggag aagccagcgt gaccatcgat aaatccatcg atgaagtgac
     7141 gccagcggag tttgatgccc tgttgctacc gggcggtcat tcaccggatt atctgcgtgg
     7201 cgacaaccgt tttgtcacct ttacccgtga ttttgtgaat agcggcaaac ctgttttcgc
     7261 catctgtcac ggtccgcagc tgctgatcag cgccgatgtg attcgggggc gcaaactgac
     7321 cgcggttaaa ccgatcatta ttgatgttaa aaacgcgggc gcggaatttt acgatcagga
     7381 agtcgtggtc gataaagatc agctggttac cagccggaca ccggacgatc tgccagcgtt
     7441 taaccgcgag gcgttacgcc tgctcggtgc ctgagccgcg tagccaaatc atttttttac
     7501 caaagcccag cgtgttgtcg gtgaatttga tttcatcggg ccggatttcc cacaccgggg
     7561 ccgacagcat tctggcaacc ggaaagcgac gattgtacgc cttgcgcgcg aggtcgcttt
     7621 cctcaccttc cagcctgcgg atctcacctt taaactgcac gccgcgaatt aacgctaccg
     7681 ttttcggctg accgtttacc gttccggcaa ccgcagcctg cggcccactc atctgcgcgt
     7741 ggcgcgtttt ttcttccgtc aaaatgtaga aggcgacttt ctgcacatca aacagataaa
     7801 aggcattggc gcaccagagt tccccttcct gctgcacaca ccaggtgaca acatgttgtt
     7861 ttgccagcca acggctgatg gcggtgagtg tttccattgc tgttctcttt tatactgtgg
     7921 gcctgacctt aacatgcgac gcagacgatg acaccctggt ttctttactt gatccgtacc
     7981 gccgacaata agctttatac cgggatcacc acggatgtcg aacgccgcta tcagcagcac
     8041 caaagcggca aaggggcgaa agcactgcgc gggaaaggag aactgacgct ggcgttttct
     8101 gcgccagtgg gcgatcgttc gctggcgtta cgggcggaat atcgcgttaa gcaactgacc
     8161 aaacggcaaa aagagcgtct ggtagcggaa ggcgcagggt ttgcaaagct gttaagcagt
     8221 ctgcaaaccc cggagattaa aagcgattga aatgctcgtg atactcaacc agaccagtta
     8281 cgccattcaa cgcgtcatcc gctaaacgat gtacctggaa ggcgctttca gtgcccggcc
     8341 agcggcaacg cagatcgtga tgcgccgcca gttcaaagcc gaaacggctg tatagcgccg
     8401 gatcgcccag cgtcaccact gcggcatagc cgaactcatt aagcgaatcg agtccttcat
     8461 agaccagttg gcgtgccagc ccctgtccac ggtatttttc atctaccgcc agcggtgcca
     8521 tgccgaccca ttgcaggtct tcgccctgca catcaaccgg actaaatgcc acatagccaa
     8581 tgacctgacc ttcgtcatct gtcgccacca gccccagcgt cagaaagcca tcttcacgca
     8641 gatcgtgaac cagcttcgct tccgcatcac tttcgaatga acgacgcagc agggcatcaa
     8701 tacccggcgc atcaatggga atttctactc gaattagcat ggttcaccta ccgatgtctg
     8761 tttggtttca ggcgcggttt tcattcccgc ctcaacaaaa tccgccagtt gcagcagcat
     8821 catgcgtaag gctttcggca tttgctccag ttcaatggcg tccatcaggt ttttcacata
     8881 cagccccagc tccgtatcgc cttcaatcac cagccgacgc tggaagaaga gcgtatccgg
     8941 atcttgttta cgcgccgcaa tcatcagcag atcgctggcg tcggcactaa aactcacatc
     9001 agcttgcgcg ttctggctga ccaccagttt gccattcacc accgaggtaa accattgcag
     9061 gtcaatatca cgcacatgaa tacttaacca gcggccttca agaaactcca gctcaccatc
     9121 atccagcgcc tggcggaatt gccagcttaa gacctgctca agaacctggc gttttagcgc
     9181 aaatggcgtc agttttaccg gtacactcaa cagagatggc cccaaatgca caatacggga
     9241 acgcagttta tccaacacga gttttactcc ctgtttcaac aatcatccta ttttgccata
     9301 tcagaaaaat aacatagcgg tataaatcaa caattccata tgaaattgct gctaccacca
     9361 atacaacttt aactgcctta aatcaaaaat tgtcgcagca aggttaacta aaatcccagt
     9421 tcgttaacat ttttgcgttt tgatagcgca accttcagga aaaattatgg agctgctctg
     9481 ccctgccgga aatctcccgg cgcttaaggc ggccatcgaa aacggcgcag atgctgttta
     9541 tatcgggcta aaagatgata ccaatgcccg tcacttcgcc ggccttaact ttaccgagaa
     9601 aaaattgcag gaagcggtga gttttgtcca tcaacatcgc cgcaaacttc acatcgcgat
     9661 taacactttt gcgcatccgg acggttacgc ccgttggcag cgtgccgtgg atatggcggc
     9721 gcagctgggt gccgacgcgc tgatcctcgc cgacctcgcc atgctagagt acgccgccga
     9781 gcgttacccg catattgagc gtcatgtgtc agtgcaggct tcggcgacca atgaagaggc
     9841 gattaacttt tatcatcgcc attttgacgt tgctcgcgtg gtgctgccgc gcgtgttgtc
     9901 gattcatcag gtgaaacaac tggcacgggt cacacctgtc ccgctggaag tctttgcttt
     9961 cggcagcctg tgcattatgt cggaaggtcg ttgctatctg tcgtcgtatc tgacgggtga
    10021 gtcgcccaac accgtgggcg cgtgttctcc ggcccgtttc gtgcgctggc agcaaacgcc
    10081 gcaggggctg gaatcccgcc tgaacgaagt gctgatcgac cgttatcagg acggcgaaaa
    10141 cgcaggttat ccgacgctat gtaaagggcg ttatctggtg gacggcgagc gctatcacgc
    10201 gctggaagaa ccaaccagtc tcaataccct ggaactgctg ccggagttaa tggcggcgaa
    10261 tattgcttcg gtgaaaattg aaggccgcca acgtagcccg gcgtatgtca gccaggtggc
    10321 gaaagtctgg cgtcaggcta tcgaccgttg taaggccgat ccgcaaaact tcgtaccgca
    10381 aagcgcgtgg atggagacgc tcgggtcgat gtccgaaggc acgcagacca cccttggcgc
    10441 gtatcaccgt aaatggcagt gagaagagca atgaaatatt ccttagggcc agtgctgtgg
    10501 tactggccaa aagagacgct ggaagatttt tatcagcagg ccgccaccag cagcgccgac
    10561 gtgatttatc ttggcgaagc ggtttgcagc aagcgccggg caaccaaagt tggcgactgg
    10621 ctggagatgg caaaatcgct cgccggaagt ggtaagcaga ttgtgctctc cacgctggcg
    10681 ctggtgcagg catcatctga actgggcgaa ctgaaacgct atgttgagaa cggtgagttt
    10741 ctgattgaag ccagcgatct cggcgtggtg aatatgtgcg ctgaacgcaa actgccgttc
    10801 gtcgccggac acgcgctgaa ctgctataac gcggtgacgc tgaaaatatt gctcaaacag
    10861 ggcatgatgc gctggtgtat gccggtggag ctttcccgcg actggctggt gaatctgctt
    10921 aatcagtgcg atgagctggg cattcgcaac cagtttgaag ttgaagtcct gagctacggt
    10981 catctaccgc tggcctactc cgctcgctgc tttaccgcgc gttcggaaga ccgcccgaaa
    11041 gatgagtgtg aaacctgctg cattaagtat ccgaacgggc gcaacgtgct gtcgcaggaa
    11101 aaccaacaag tgtttgtact caatggcatt cagaccatga gcggctacgt ttacaacctc
    11161 ggtaacgagc tggcatccat gcagggcctg gttgatgtgg tccgcctgtc accgcagggt
    11221 actgacactt tcgcgatgct cgacgccttc cgcgctaatg aaaatggcgc tgcaccactg
    11281 ccgttgacgg cgaacagcga ctgtaacggc tactggcggc ggctggcagg actggagctg
    11341 caagcctaag taaatagctc actttgttaa caactttaac tactctttaa tgcagtatta
    11401 aagattaatc ggtaacaaag tgagctgtta tgactgataa aaccattgcg ttttcgctac
    11461 tcgatctggc ccccattccc gaaggttctt cagcgcgaga tgcattctcc cactctctcg
    11521 atctcgcccg tctggctgaa aagcgcggct atcatcgcta ctggctggca gaacaccaca
    11581 atatgactgg cattgccagt gctgccacgt cggtattgat tggctatctg gcggcgaata
    11641 ccaccacgct gcatctgggg tctggcggcg tgatgttgcc taaccactca ccgttggtca
    11701 ttgcagaaca gttcggcacg ctcaatacac tctatccggg gcgaatcgat ttggggctgg
    11761 gtcgtgctcc gggtagtgac cagcgaacca tgatggcgct acgtcgtcat atgagcgggg
    11821 atattgataa tttcccccgc gatgtcgcgg agctggtggc ctggtttgac gcccgcgatc
    11881 ccaatccgca tgtgcgcccg gtaccaggct acggcgagca aatccccgtg tggttgttag
    11941 gctccagcct ttacagcgcg caactggcgg cgcagcttgg tctgccgttt gcgtttgcct
    12001 cacacttcgc gccggatatg ttgttccagg cgctgcatct ttatcgcagc aacttcaaac
    12061 cgtcggcacg actggaaaaa ccatacgcga tggtgtgcat caatattatc gctgccgaca
    12121 gcaaccgcga cgctgaattt ctgtttacct caatgcagca agcctttgtg aagctgcgcc
    12181 gtggcgaaac cgggcaactg ccgccgccga ttcaaaatat ggatcagttc tggtcaccgt
    12241 ctgagcagta tggcgtgcaa caggcgctga gtatgtcgct ggtgggcgat aaagcgaaag
    12301 tgcgtcatgg tttgcagtcg atcctgcgcg aaaccgacgc cgatgagatt atggttaacg
    12361 ggcagatttt cgaccaccag gcgcgcctgc attcgtttga gctggcgatg gatgttaagg
    12421 aagagttgtt gggatagtgt gtcttaacgc gggaagcctt atccgagctg gcaacgctgt
    12481 cccacgtagg cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga
    12541 taaggcccgc tgattactga tacaccggca gcaaattaaa gctcgataag atatggacca
    12601 gtgcgttgcc aacgccaaat accagaatca gcgtaatcat cggcttgccg ccccagacgc
    12661 ggaatttcgg gctgccaaag cgtttacgcg atgcgcgggc cagcagtgcc ggaacaattg
    12721 ccgcccagat ggtagctgct aaaccagcat aaccaatggc gtacaggaat ccgttcggga
    12781 acagcagacc acccacaaca ggaggggcaa aggtcagcaa tgccgttttc aagcggccca
    12841 cagccgagtc gtcgaaacca aacagatctg ccagatagtc aaacaaaccc agcgttacgc
    12901 cgaggaacga actcgctacc gcaaagtttg agaacacgac cagcagcaga tccagactac
    12961 ggctgttcag tacgccgctt aacgcctgta ccagcacatc aatattaccg cccttctctg
    13021 caatatcgat aaactccgga cgcgggatgt tacccatcgt cgccagcaac cagatggtat
    13081 acagcgccag cgccatcagc gtaccgtaca ccagacattt cacgatggtt ttcggatctt
    13141 tgccgtaata cttcatcagg cttggcacgt taccgtgata accaaacgat gccagacaga
    13201 acggcaaggt catcaacaga tacggtgcat aagacgcatt gctttcagcg acgttgaaca
    13261 atgtcgcagg ctgcacatgc cccagcaggc taccgaaggt gaggaagaag gtaatgactt
    13321 tcgcccccaa tacaatcgct gtcatgcgac tgacggcttt agtgctcaac cacaccacaa
    13381 acgctaccag caatgcaaaa ccaaaacccg ccgcccgtgc cgggacgttt agtgacatct
    13441 ctgcgaaggt gtgatgcaga atcgaaccac tggcagaaat ataggcatag gtcaggatat
    13501 agagcacaaa ggcaatggaa atgccgttga ccacgttcca gcctttgccc agcaaatctt
    13561 tggtgatggt gtcaaaactc gaaccgattc tgtaattcag gttagcttcc agaatcatca
    13621 agccggaatg cagcatacag aaccaggtaa agatcagcgc cgccattgac cagaaaaacc
    13681 acgccccgga catgaccact ggcagagaaa acatccctgc gccaataatg gtgccgccga
    13741 taatcaccac gccgccaagc agcgacggtg acgtttgggt ggtggttagt gttgccatga
    13801 gggcttctct ccagtgaaaa atagtgcgac tgcgttgtta tgtattgcac tgtaccagta
    13861 cgcgagtaca aaagacagaa aaaaagcccc gatggttcaa atcggggctg tatatattat
    13921 tttacagact gtgttcgctg ttcagcgatg attacgcatc accaccgaaa cgacgacgac
    13981 cggtagagtc atcacgacgc ggagcgcggc cttcacgacg ttcgccgcta aaacgacgac
    14041 catcaccacg accaccttca cggcgttcac cgctgaagtt acgaccacct tcacgacgct
    14101 cgccactgaa accacgacca ccgccacgac gctctccgcc agtatgcggc tgtgcatcgc
    14161 ccagtaattg catattcatc ggcttgttca gaatgcgagt acgtgtaaag tgttgcagca
    14221 cttcacccgg catacctttc ggcagttcga tggtggagtg agaagcaaac agcttaatgt
    14281 taccgatgta acggctgctg atgtcgcctt cgttagcaat cgcaccaacg atatgacgaa
    14341 cttcaacacc atcatcgcgg cccacttcaa tacggtacaa ctgcatgtca ccaacatcac
    14401 gacgttcacg acgcggacga tcttcacggt cgccacgcgg accacggtcg ttacgatcgc
    14461 gcggaccacg gtcatcacgg tcacggaatt cacgtttcgg acgcatcggc gcatctggcg
    14521 gaacaatcag agtacgttca ccctgtgcca ttttcagcag tgcggcggcc agagtttcga
    14581 gatccagctc ttcaccttct gcagtcggct gaattttgct cagcagtgcg cggtattgat
    14641 ccagatcgct gctttccagc tgctgctgta ctttagcggc gaatttttcc agacggcgtt
    14701 tgcccagcag ttctgcgttc ggcagttcta cttccggaat agtcagcttc atagtacgtt
    14761 caatgttgcg cagcagacga cgctcgcggt tctcaacgaa cagcagcgcg cggccagcac
    14821 gacccgcacg accggtacga ccgatacggt gaacgtaaga ctcagaatcc atcgggatat
    14881 cgtagttaac caccaggctg atacgctcaa cgtccaggcc acgggctgca acgtcggtcg
    14941 caatcaggat gtccagacga ccatctttca ggcgttccag tgtctgctca cgcagcgcct
    15001 ggttcatgtc accgttcagc gcggcgctgt tgtagccgtt acgctcaaga gcttcagcca
    15061 cttccagagt cgcatttttg gtacgaacga agataatcgc cgcatcaaaa tcttccgctt
    15121 ccaggaaacg taccagtgct tcgtttttac gcatacccca gacagtccaa tagctctggc
    15181 tgatgtcagg acgggtagtc acgctggact gaatgcgcac ttcctgcggc tctttcataa
    15241 agcggcgggt aatgcgacga atcgcttccg gcatggttgc agagaacaga gcggtctgat
    15301 gaccttccgg gatctgcgcc ataatagttt caacgtcttc gatgaagccc atgcgcagca
    15361 tttcgtcagc ttcatccaga accagaccgc tcagtttaga gaggtccaga gtgccacgtt
    15421 tcaggtggtc cagcagacgg cccggagtac cgacaacgat ctgcggcccc tgacgcaggg
    15481 cgcgtaattg cacgtcataa cgctggccgc cgtacagggc aaccacattt acgccgcgca
    15541 tgtgtttaga gaaatccgtc attgcttcag caacctgtac cgccagttcg cgggtcggtg
    15601 ctagcaccag aatctgtggt gctttcagct caggatcaag attttgcagc agaggtaaag
    15661 agaatgctgc agtttttccg ctccccgtct gggccatacc cagtacgtcg cggccattca
    15721 gcagatgtgg aatacactcc gcctgaattg gagatggttt ttcgtaaccc agatcgttaa
    15781 gggcttcaag gataggagcc ttcaggccca gatctgcaaa agtggtttcg aattcagcca
    15841 tgtagtacgt gtgcctcaaa attaatggcg gccagtctac ataactcatc atgaaattga
    15901 tcagcaattt tcattgaaaa gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa
    15961 cgccctcacc cgttaaggtg atggcaatca aaaaagatta cgggctgatg tgtacgtcag
    16021 ctattgctgg tccgattctg ccaggtcatc ttggtcctgg cccaggagcg ataattccaa
    16081 caatgcgtat cggtgctcaa caaagttatg aacgttgttg gcaaccgcca gtttgaacag
    16141 tgccgtggcg ctgtccaaat cccccagact taggtagtac ttacctaaat agaagttggt
    16201 ttcactgaga tgctcagcga gcgaggtgtt atccgttgcg tccgccttga gcctttccat
    16261 taacgtttgt tcgctaatgt tgcccaggta gaactcgaca atgttccatc cccactgttc
    16321 cttatccgat ttttcgaagt gctgtttcaa cacttcttta gcctgcttct catcgagctt
    16381 ctgctcggcg agataaagcc acagactacg gaaaggatca ttgggatcgt cttgataaaa
    16441 cgccagcaga tcatcttgcg ctaacttgtc acgaccgccg taatataatg cgatcccgcg
    16501 attcaagtgc gcgtagttgt aagttggatc aagctcaagt acagaatcaa acgcttcata
    16561 ggcagcatca aaattgcctg cctgcgttaa atatatgcct aagtaattga atacttcagg
    16621 catatccggt cggattgcca gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc
    16681 gagactatca tacaacactc cgcgctcata taaaagctgt gcgcgttcgt catcggttaa
    16741 agcccgactg gcaaggattt gttccatacg tgccagaatc acttcctgct gtaaagtcgg
    16801 ttgcaatggt accgcgagga cttcactttt acgccaggaa gtattactgc atcctgcaag
    16861 cgtaagtgct gtcgcaacga aacaccagcg caaaaaaggc ttcatttccc actcccgaag
    16921 accacggttg aatgaacgtc ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa
    16981 gccccccgcc gcagcggagg gcaaatggca accttactcg ccctgttcag cagccggagc
    17041 ttccggtgct gcagcaggtt gagactgctc agtcgcttct ttaatgctca gacggatacg
    17101 gccctggcga tcaacttcca gaactttcac cggtacttcc tgacccatct gcaggtaatc
    17161 ggtcactttc tcaacgcgtt tgtcagcgat ttgagagatg tggaccagac cttctttacc
    17221 gccgccgatg gcaacaaatg cgccgaagtc aacgatacgg gtcactttac cagtgtagac
    17281 gcggcccact tcaatttctg cagtgatctc ttcgatacga cgaatagcgt gtttcgcttt
    17341 ctcgccatcg gtcgctgcga tcttcacagt accgtcatct tcgatttcga tggtggtgcc
    17401 agtttcttcg gtcagggcac ggatcacaga gccgccttta ccgataacgt ctttgatctt
    17461 gtccgggttg atcttgatgg tatggatacg cggagcgaac tcagagatat cgccacgtgg
    17521 cgcgttgatc gcctgttcca ttacacccag gatgtgcaga cgcgcacctt tagcctggtt
    17581 cagtgcaacc tgcatgatct ctttggtgat accttcaatt ttgatatcca tctgcagtgc
    17641 agagataccg tcgcgggaac ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc
    17701 gcccaaaatg tcagacagta caacgtagtt gtcgccttct ttcaccagac ccattgcgat
    17761 acccgcaacg gcagctttga ttggcacacc tgcgtccatc agcgccagag acgcaccgca
    17821 cacggaagcc atagaagaag aaccgttgga ttcagtgatt tcagacacaa cacgtacggt
    17881 gtacgggaat ttgtccatat ccggcattac tgccagcacg ccgcgcttcg ccagacgacc
    17941 gtgaccaatt tcacgacgct tcggagaacc gaccatgccg gtttcgccta cggagtacgg
    18001 agggaagttg tagtggaaca ggaaggtatc ggtacgttcg cccatcagtt catcaagaac
    18061 ctgcgcgtca cgagcagtac ccagcgttgc ggtaaccagc gcctgcgttt caccacgggt
    18121 gaacagcgca gaaccgtgag tacgcggcag cacgccagta cgcacatcca gaccacggat
    18181 catatctttt tcacgaccat cgatacgcgg ttcgcctgcc agtacgcggc tacgaacaac
    18241 gtttttctcg atcgcgtgca gaatttcacc cagttcgttt tcgtccaggg tttcgtcttc
    18301 agcaagcagc gtcgcgatgg tttcagattt gatgacatca acctgcgcat aacgctcttg
    18361 tttgtcggtg atgcggtaag catcgctcag acgagcttca gccagtgcag caacgcgcgc
    18421 gttcagcgct tcgtttaccg gctccggctg ccagtcccaa cgcggtttac cggcttcttt
    18481 caccagttca ttgatgttct gaataacaac ctgctgttgt tcatgaccga acactactgc
    18541 gcccagcatc tggtcttcgc tcagcagttc agcttcagat tcaaccatca gtacagcggc
    18601 ttcagtaccc gcaacaacca gatccagttt gctctctttc agctcgtcct gagtcgggtt
    18661 cagtacgtac tggtcattga tgtaacctac gcgggcagca ccaatcgggc cattgaacgg
    18721 aataccagac agagacagtg ctgcggaagc accaatcatc gcgacgatat ccgggttaac
    18781 ctgcgggtta acagaaacca cggtggcgat aacctgaact tcgttgacga agccttccgg
    18841 gaacagcggg cgaatcgggc ggtcaatcag acgcgcgatc agggtttcgc cttcgcttgg
    18901 acgaccttca cgacggaaga agctacccgg gatacgacca gcagcgtagg tacgctcctg
    18961 atagttaacg gtcagtggga agaagtcctg acctggtttg gcttttttct ggccaacaac
    19021 ggtaacaaat accgcggtgt catccatgct aaccataaca gcggcagtag cctgacgagc
    19081 catcatgccg gtttccagag tcacggtgtg ttggccgtac tggaatttac gaacgatcgg
    19141 attaagcaat gtaatatcct ttctctttct tagacagtac cttacggcac tggtgttaat
    19201 acccgatctt ctgcgcatcc tcgcgactaa tgacaaccct aacccagctc tatgtgggta
    19261 aagcctctca ttagccgcgc gaacctctgc aacggaagat cattcatagc aacaatacat
    19321 tagtttccag tgaattgctg ccgtcagctt gaaaaaaggg gccactcagg cccccttttc
    19381 tgaaactcgc aagaattagc gacgcagacc caggcgctcg atgagccggg tgtaacgtgc
    19441 tacgtcttta cgtttcaggt agtcgagcag tttacgacgc tgagaaacca tgcgcagcag
    19501 accacgacgg ctgtggtgat cttttttgtg ctctgcaaag tggccctgca ggtggttgat
    19561 ctgtgcagtc agcagtgcta cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc
    19621 aaactcagaa acgattttag ctgttgcttc agtacttaga gacattttaa aactccaaag
    19681 tatatagaat gaaaggacgc cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag
    19741 tgttaaacaa tttacgcgac gttaagcggc agtattctac tcgtagcgac ctgttatcgc
    19801 aagacggtta acattacgcc gggtattcaa ccaccagacg acgaggcgca acgcggcctt
    19861 catcgtcaat ttcgcccata ccgataaact tgccgttctc accttccgta acgcgaacca
    19921 gtccttccag cggcgcgcca gatgtacgaa ccgggttacc atttttgaag taaacagaag
    19981 acgttaacgg aagattcacc accgggtagt ccgaagctgg actgtccatt ggcatcagta
    20041 atggatcaag caactccgca gccggaatat cctgctgttc ggcttgttcg accagttcac
    20101 gcaggtgctc cagggtcacc atccgttcaa ccggatattt acttaccgcc agacgacgca
    20161 ggtaaatcac atgcgcgcca cagccgagtt tttcacccag gtcatcaatg atggtgcgga
    20221 tataagtgcc ttttgagcag tgaatttcca gctccagctc attgccttca tggcgaataa
    20281 acagcaattc ataaacggta attggacgcg cttcacgcgg aacttcaatg ccctgacgcg
    20341 catattcgta cagttttttg ccctggtatt tcagtgctga atacatcgat gggatctgtt
    20401 cgatatcgcc acggaaagta tccagtgccg ccgccagttg ttctgcacta aaggttaccg
    20461 gacgctcttc aacgatttgt ccgtcggcgt cagaagtatc ggtacgctgt ccaagacgcg
    20521 caatgacccg atagcgtttg tcggagtcca gcagatactg ggaaaacttc gtcgcttccc
    20581 cgaggcaaat cggcaacatg ccggtcgcca gcgggtccag cgcaccggta tgcccggcac
    20641 gattggcgtt atatatacgt ttcacttttt gcagcgcatc gttgctggac ataccctgcg
    20701 gtttatccag caacaaaacg ccgttaatgt cgcgaccgcg acgacgagga cgactcatta
    20761 gtcctccttg ctgtcgtccg ggttaacacg acgttcttcg tcatgtttga ccacgctggt
    20821 caccaggttt gacatacgca tcccttcaac cagagagttg tcgtagaaga aggtcagttc
    20881 cggcacgata cgcaggcgca tcgctttacc cagcaggctg cggatgaaac cagaagcttc
    20941 ctgcaacgct ttgatgcccg ctttaaccgc gtcttcatct ttgtcgttga ggaaagtcac
    21001 atacactttg gcatacgcca ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat
    21061 gcccagacga ggatctttaa tttcacgctg caggatgaga gcgatctctt tttgcatttc
    21121 ctgcgctacg cgctgcgggc gaccaaattc tttcgccata ataaattctc ctgacaaaaa
    21181 aggggctgtt agcccctttt taaaaattaa tttcaggtgg aagggctgtt cacgttggcc
    21241 tgataagacg cgccagcgtc acatcaggca atccatgccg gatgcggcgt aaacgcctta
    21301 tcccgcatgg aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat
    21361 acttcgatca catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc
    21421 ataccgttac ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct
    21481 tcatagataa ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct
    21541 tcggtaacca tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact
    21601 tccgccagac cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct
    21661 ttcacttcgt cgatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct
    21721 tcaatcactt tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg
    21781 gacgccgcag ccaaggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc
    21841 ttcactttaa cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttcgacagaa
    21901 ccctgtacgt ctgccttcag gacgatattc acttcgtgaa cttcgccttc ggtcatgttg
    21961 gcgaacatgt tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta
    22021 ccctgacgat agagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca
    22081 tcacccgcag ccggaacacc ggacaagccg aggatttcca ccggaatgga cggacccgct
    22141 tccagcactt cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca
    22201 cacagaacga tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga
    22261 ccacgacctt tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc
    22321 gctttcagct ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgataccg
    22381 gtacccgctt tcgcagatac gtgtacgaac tggctttcac cgccccactc ttccggcagg
    22441 atgccgtact gggagagttc gttcttaacg cgatccggat cggcttctgg tttatcgatc
    22501 ttgttcactg caaccaccac cggtacctgc gccgctttcg cgtgctggat tgcttcgatg
    22561 gtctgcggca ttacgccgtc gtcggcagca acaaccagga ctacgatgtc agttgcctgc
    22621 gcaccacgtg cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc
    22681 atgccgtttt cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg
    22741 ccagaggcca ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg
    22801 tgacccatga tggtcacaac cggagcgcgc ggttcagccg cagctcccgt gtcacggtcg
    22861 ctcattaccg cctcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct
    22921 tcagcaacca gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc
    22981 agtttcatca tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg
    23041 ccaacggtga tagtttcgcc gatcacaacg tcacggttaa cggcctgagc aggcttctgg
    23101 aagccttgct gcagcgaaga acctttacgt tttccgccct taccgccacg tactgctgcg
    23161 cgtgcttctt cacgatcagc ttttgattca gcgtgtttgt tgcctttctt cggacgcgct
    23221 gctttcgcgt tacgaccacg gccacggccg ccttcgactt cacgatcgct ttcgtcttct
    23281 gcctggcgag catgttgaga agtagtgacg tgataatcgc tggaatcctc agtcggttcc
    23341 gcgttatcag tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt
    23401 gcttcttctt cgagtttacg acgcgcttct tcttcggctt tacgcttgag ctctgcagct
    23461 tcctgctcac ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg
    23521 ctcactttgt ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct
    23581 tgttctgcgg cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc
    23641 tcacgacggg cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca
    23701 gcctcttgcg gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat
    23761 ttgctttttc caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc
    23821 aatttgtccg ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt
    23881 gcagacacag agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg
    23941 cgttccacgg aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg
    24001 ttccttcctg ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc
    24061 ataatcagtg ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg
    24121 ccctgttcgg cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa
    24181 tcacgatcta ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt
    24241 tcttcctggg cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca
    24301 acggtcggct catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt
    24361 tcttccagcg tcgagaagcc ttcctctacc agaacagtcg cgaagtcttc gtcgatgtcg
    24421 agatatttgg tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg
    24481 tcgtcaacgg tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc
    24541 tgaccgttac ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg
    24601 tgtttatctt catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca
    24661 aactgcgccg ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta
    24721 gaaaccgcct gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt
    24781 ttatcgttgg ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta
    24841 atttcaatca cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt
    24901 tccggcttgg aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa
    24961 agcacgccac gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc
    25021 aggatcacgg cttcagcgtt gttgcccaga tccagagaga tgttgtcgcg gtttactttt
    25081 ttcaccacgc cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca
    25141 cgttcggctt cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg
    25201 tcaaaggtaa cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct
    25261 tcataacgtg cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact
    25321 aaccagcgac ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg
    25381 atctcttgtt catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa
    25441 atcttctcgc gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct
    25501 ttgttcatcg cgggcttttc acctcatcca gactattaaa agtggggaac caagttcgcc
    25561 ttctggatat tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt
    25621 tcaccgtcta ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg
    25681 agaaccagag tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg
    25741 cgatcgagac ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca
    25801 tccagcacag cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct
    25861 tcactatcaa tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc
    25921 agttcaaaac ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat
    25981 gtggacaagc ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc
    26041 agataacaaa aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact
    26101 gcggtctgga gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt
    26161 cacagtatat ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg
    26221 acgaccttcg ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg
    26281 tggcaaattc tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga
    26341 gacgggacgt aaaatctgca ggcattatag tgatccacgc cacattttgt caacgtttat
    26401 tgctaatcac gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc
    26461 ctgctctccc ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat
    26521 aaaagatgat taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc
    26581 caggctcatc atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc
    26641 tcccggtagg tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac
    26701 tgctgtggat gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc
    26761 cagacgaaga ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg
    26821 cacgtctgat cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg
    26881 gcgcatttca taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg
    26941 ccgtgactgg taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg
    27001 acggtagcac ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca
    27061 atgctgaact gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg
    27121 gtcgtcatga aatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg
    27181 aaaaagctta ctccacggac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg
    27241 aatacctcaa ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg
    27301 agagcgtgaa gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtgg
    27361 cgttgaacgg taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg
    27421 gcggtcgtca cggcctgggc atgagcgacc agattgaaaa ccgtatcatc gaagcgaaaa
    27481 gccgtggtat ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgcctgt
    27541 tgaccggtat tcacaacgaa gacaccattg agcagtatca cgcgcatggc cgtcagttgg
    27601 gccgtctgct gtaccagggg cgttggtttg attcccaggc gctgatgctg cgtgactctc
    27661 tgcaacgctg ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga
    27721 acgattattc aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga
    27781 cgatggaaaa aggcgactcg gtgttctcgc cagatgatcg tatcggtcaa ctgaccatgc
    27841 gcaacctgga tatcaccgat acccgcgaga aacttttcgg ttatgccaaa actggcctgc
    27901 tttcctcctc tgccgcttca ggcgtgccgc agatggagaa tctggaaaac aaaggccagt
    27961 aattcgacat caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc
    28021 ctttctgagc atcacataat cgaaaacatc atggtttgca tccacaacca gtgagtctcc
    28081 tttatagtgt acaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat
    28141 ccacgcatta ataaggtaat tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg
    28201 atccacacca tccccaagaa cggggctata ccagataaac atcggaacat gatatgcctg
    28261 ctggctggcc tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag
    28321 gccgtggtcg gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg
    28381 acctagcaaa ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg
    28441 cggttgaaac acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt
    28501 taaatgaaga acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg
    28561 cggcaacaac aattcatcaa atcctctgac ataaactgtt tccatggcac gcatggcgat
    28621 actggtaact gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg
    28681 aaatcctgct tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatggct
    28741 caaaacagaa tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcaccgct
    28801 tattgcttga ttaaacagtt tgatctgttt tctttgtgct tcgacttgcg gtgtcgtaga
    28861 gcgtgtatat ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac
    28921 gtaggtatca atacctgtat ccctgactga taattgaaaa tacggcactg tgtttgcaat
    28981 tgagagtaat ctttgatgct ctttcgctgc taaagcaaaa taattgagat tgaaaaacgg
    29041 cgtataggta gcaaatcgcg acgctagtat atatggactg aacgcatttt tatttttggc
    29101 atctttataa gcaaattgac aagcggaaaa taaacttccc gaaatgacaa tcagcaataa
    29161 tattcctgtc actttttttg tcggcaagga aacatcatat tttattatta cagccaaaaa
    29221 aagaagggat aaaaaggcaa aagcacatag ataagggcta tacattccca gcattttggc
    29281 aacttcatcc ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc
    29341 aaaggtacac catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg
    29401 tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt ttaacaacca ggatggtcag
    29461 aacgaagaac agcaatcgct gaaccatatg cgctccggaa gaggcgacta agttcgtaat
    29521 accaaaaaca ataacactaa gatacaacaa ccaatgagat ttaaaacttc tagcaaattt
    29581 gttgaatact gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg
    29641 tgcaatttta tatcagacat gtttttcaac tctgatccat atcagaacga aacactgtta
    29701 gttttttctt tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat
    29761 gcgtctctaa acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg
    29821 gtttatcgag aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt
    29881 gccaattatg tcagcagtag ggaaaagttt ataacgaagg cgtaccggca ccatcgtttc
    29941 aaggtaccag ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc
    30001 agaccaggac caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc
    30061 gaggacggga cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct
    30121 accaattcca ccacctcggc acggaaacta ctttttagtt cgggatatcg ctggtcggct
    30181 tagccggagc agctggctga gtttgttcgg ttttcgccgg cgcactcagg ttttcccatt
    30241 cgctaccttt attggtcttg ttgctattga tgttacccag caccagactg atgatgaaga
    30301 ataacgttgc cagcaacgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa
    30361 acagcgtagc ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct
    30421 gtagcatgat cagaccaaca aggccaattg ccacaataag gaaaactacc aaaagagctt
    30481 cgtacataat caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag
    30541 cgggagttta taatctttcc cactgaagcg ggtgtgaata ctaaccaaaa cgaatgacct
    30601 tcgcaagggc aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg
    30661 ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac
    30721 ggcttttact gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc
    30781 ttccaccatc acgcgaatta acggttcggt gccggattta cgcaacagca cacgtccacg
    30841 gctacccagc gccgtttcaa cttctgcggt caccgctttg actgattcat gctccagcgg
    30901 atcgccgcta cctgcggtgt aacgcacgtt taccaggatt tgcgggaaca ttttcatgcc
    30961 gctgcaaagg tcgtgcaggc tcatatggtt acgcgccatc gccgccagca cctgcaaacc
    31021 agcaacgatg ccgtcaccgg tagtggtttt atccagcagg atcacatgac cggaattctc
    31081 tgcaccgata cgccagcctt tctcctgcat tttttccagt acgtagcggt cacccacttt
    31141 cgcgcgcgca aatggaattc ctaactgttt cagcgccagt tcaagcccca tgttgctcat
    31201 caatgtaccc acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat
    31261 atacatgatc tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc
    31321 gccgtcgccg tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag
    31381 cgcgcgaacg tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc
    31441 gatagcgata acattcgctc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc
    31501 accgtttgcg caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt
    31561 ggctttgcaa aactcgatat agcgacccgc agcatcaacg atacggctgg ctttacccag
    31621 ttctgccgaa tcaacacagc tgatctcttt ttccatttcc gcttcgatgg cttcttcaac
    31681 tgcatccggc agcttagtac cgtcaataga aaagaattta atgccattat cgtagaacgg
    31741 gttatgcgat gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc
    31801 cacggccggt gttggcatcg ggccagtgaa gagtgcggaa aggcccgcag ccgccagccc
    31861 cgcttccagt gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat
    31921 cttacgggag ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa
    31981 atcaggtgtg atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt
    32041 acgattactc atagcgtttg ttttcctttg cagacagagt agcttccacc actcgcatcg
    32101 cttctacggt ttctttgacg tcatgaacac gaataatgtg cgcgccttgc attgcggcaa
    32161 tgaccgcaca ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc
    32221 caatcatcga ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag
    32281 ccaggcgcgc cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt
    32341 cgagcaacaa tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa
    32401 agtagcgatt cacttctgca aagacatcgt catacttcgg ggcttcctgc atggtttttg
    32461 gatttccctg catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag
    32521 cgccaggttc ggaaagggag cggatatcgt taataatgtg agcgccaact ttcgctgact
    32581 cacggatgac ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa
    32641 ttgcctcaac cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc
    32701 ctggccgcgt ggactcgcca ccaacgtcaa tgatcgtcgc gccagcgttg atcatcagat
    32761 tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag
    32821 gcgtgacgtt gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg
    32881 caaagagttt catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc
    32941 cccggggttt tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg
    33001 ctctgacatg gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt
    33061 tggactacca ttgtcgccag cattgttaga agcgcctggt tcttcccagc ccgctggcgg
    33121 acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt
    33181 catgagagca tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc
    33241 acgattatag ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc
    33301 atcggacata tgtttcgctt tcgctacgct acggccgagg aacacttcac cttcttcttc
    33361 cgcgtacagc agtggaccca atttctcaga gaagccccac tgagtcacca tgttacgtgc
    33421 caggttggtc gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccgta
    33481 gatgatctct tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg
    33541 acggctggcg ctgattgcgt cgccctcagg caagaagaaa gtcacaccca gcgcacgacc
    33601 gcgtgggata atcgtcactt tgtgcaccgg atcgtgctcc ggcaccaggc gaccgataat
    33661 cgcatgaccc gcttcgtggt aagccgtcga ttctttctgc gcttccgtca tcaccatgga
    33721 gcgacgttcc gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac
    33781 aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc
    33841 agcaccggag aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggtgccaa
    33901 tggtacgcga cgcatgtgaa ccttcaggat ctgctcacga ccgcgaacat ctggcaagcc
    33961 aaccacaacc tgacggtcga aacggccagg acgcagcagc gctgggtcga gaacgtccgg
    34021 acggttagtc gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac
    34081 cagcatctgg ttcagagtct gttcacgttc atcgtgacca ccgcccagac ctgcgccacg
    34141 ctggcggcct acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc
    34201 ctgttcgaac atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc
    34261 agaaccggag atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcag
    34321 cgttttaccg gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag
    34381 tttctggaag cggctcggct cgcgcagata ctcaaccagt tcagcaactt cttcttttgc
    34441 ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg
    34501 cgctttactc ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat
    34561 gaagaagatc cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc
    34621 cagcaggctg ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt
    34681 atccagtaat ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt
    34741 ggtaacgttg atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc
    34801 ttgtaggaag gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg
    34861 gaatactgac atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc
    34921 actcaaggga ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat
    34981 ccagcatctt tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac
    35041 aatatacact tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt
    35101 aaacagggag cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac
    35161 tacaaaactg ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag
    35221 atacatggca cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga
    35281 catgacaacc tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc
    35341 atcacgaaaa tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc
    35401 gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc
    35461 cggagcagca ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg
    35521 tatttcatca agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac
    35581 atatttatcg ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc
    35641 tgtcatttaa ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg
    35701 cctatttggt gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag
    35761 caaaaaatac gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc
    35821 cgctcaagcc agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga
    35881 ttgaacaagc gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg
    35941 aaactaaaac cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg
    36001 tcatcggtaa aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac
    36061 gctaagatta tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc
    36121 aggagagcaa aatgccacgc tctgttcgtt gataaaaggc cgctatgcgg cctttttcct
    36181 ttctttacaa tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa
    36241 ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt
    36301 tgccgatcag gccacgagca ataggtgagt ttacagaaat caggttttgt ttaaagtcag
    36361 cttcgtcatc gccaacgatg cgataagtct gttcttcgtc agaatcgaga ttcagcaccg
    36421 ttacggtagc accaaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct
    36481 gcgcgttcga cagcttggct tcgatgtctt tgatacggcc ttcgcagaag ccctgctgtt
    36541 cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga
    36601 tagcagcaat gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt
    36661 tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc
    36721 tgatggggca agtgttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg
    36781 ggaccagaag caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat
    36841 tttgatagtc attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg
    36901 cgccgtagta tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat
    36961 ttatcatcgg attgaccagc tgtatagcgt tcagtgttca ggccgcaaat gttgatgagt
    37021 acattactca actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt
    37081 cggcccccgc tattgattac cacagtcagc agatggcgct gcccgccagt acccagaaag
    37141 tgattactgc gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc
    37201 ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg
    37261 gtgccgatcc gacgttaaaa cgtcaagata ttcgcaatat ggtcgcgact ttgaaaaaat
    37321 ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg
    37381 ataaagcccc cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg
    37441 tcgccatagt tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg
    37501 atatggcttt tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca
    37561 ccctcccccg tggttctgcc gaagcgcaat actgcgaact ggatgtggtg ccaggcgacc
    37621 tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt
    37681 ttgccgtgca ggatggagcc agctatgccg gtgcaattct gaaagatgag ttaaaacagg
    37741 cgggtatcac ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg
    37801 tagttgccag taaacagtcg gccccgctgc acgatctgct taagattatg ctgaaaaagt
    37861 cggacaacat gatcgccgat acggttttcc gcatgatagg ccatgcgcgc ttcaatgtgc
    37921 ctggaacatg gcgggccggg tcggacgctg tgcgtcagat cctgcgccag caagccggtg
    37981 tcgatattgg aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg
    38041 cccccgccac catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact
    38101 ttatctccat gctgccgcta gcgggctatg acggctcttt gcagtaccgt gcaggtctgc
    38161 atcaggcggg cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata
    38221 acctggcggg attcattacc acagcgagcg ggcaacgaat ggcgtttgta caatatcttt
    38281 ctggctatgc agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg
    38341 aaagccgttt gtataaagat atttatcaga acaattagtc aaaaagaaac cccggcacat
    38401 ggccggggtt ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg
    38461 tcccagtcgt catcccagtc ctcatcatcc tcttcagcaa tctcttcaag ctgctggcga
    38521 tgataatcat cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg
    38581 accgggtttt caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtcca
    38641 ctcgccgcag agatcagata atatttatct tcccagccca gcgcctctgc gatcgctttc
    38701 gctttctctt cggcttctac cttatccagc agatcgatct tgttgaagac taaccaacgc
    38761 ggtttcgccg ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt
    38821 tcaaccggat cggtgccgtc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa
    38881 cgttccaggt gcttcaggaa gcgaatgccc agacctgcgc cttccgcagc gccttcaatc
    38941 agtcctggaa tatcggcaac aacgaagctc ttttcgttgt ccattcgtac cacacccaga
    39001 cttggcacca gagtggtaaa cggataatcc gccactttcg gtttagccgc cgataccgca
    39061 cgaataaagg tcgatttacc cgcgtttggc atccccaaca taccgacgtc agccagcagc
    39121 atcagctcca gcagcagctc acgcttatcg cccggcgtac cgttagtttt ctgccgtggt
    39181 gtacggttaa ctgacgattt gaaacgggta ttgcccagac cgtgccagcc gcccttagca
    39241 accagcagac gctgaccgtg tttggtcata tcgcccatgg tttcaccagt accctggtcg
    39301 attacacgcg tacctaccgg cactttaatt gtgacgtctt taccgcgctt accggtacag
    39361 tcgcggcttg cgccattctg accgcgctct gcacggaaag atttttcaaa acgataatcg
    39421 ataagcgtgt tcaggttctc gtcggcttcc atccatacgt caccaccatc accgccgtcg
    39481 ccgccatccg ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta
    39541 ccgccatcac ctgcaacgac cagaatcgat gcttcatcaa caaacttcat tttattctcc
    39601 gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg tatgcttacg taatccgccc
    39661 caaatacgat gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga
    39721 taacctaaaa ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga
    39781 tctgaaaaaa acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc
    39841 tgccagcgag ccatcgcttc cgccagggcg ccatatccta ccaaggtatt cagtccgcaa
    39901 aaaattaaac atgcgagctg ccagtggcta agctgcgcta tcactccagg ctttgccaga
    39961 gggaagagcg caattgtaca taaagtgtac agtaaaaaca ggatctgcgg tgaggccagc
    40021 cgacgcaata aaaccttttg cgccacgcca taactcaccc aaaccgtcgc agcaccgacc
    40081 ccaaagataa ctccccaggt gtaatcggtt agctttgtaa atatctcgac cagactggtg
    40141 ttaaaaaaca tcaccaggcc gctcaggagc atcaatgccc ctacaacctg agtgctgcgc
    40201 attttctctt tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca
    40261 atcacctgcg aagcggtcgg actcaggtat tgcaaggatg agctgaacag gatgaagttc
    40321 ccaaacagcc cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg
    40381 cgtaatggcg gcaacctctt cttcaccgca agaatggccc ccaggccaat actcgccatc
    40441 aagaaacggt aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgca
    40501 attggcaacg ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc
    40561 tgctgcttca taccgtattc cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc
    40621 atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt
    40681 accgggacgc gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct
    40741 ttaacttcga atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa
    40801 cctacgttag cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc
    40861 agaacggatt cgccaccgaa acgcttaacg cccaggcgtt tagcttctga atcgcgaccg
    40921 ttacgtgtgg agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg
    40981 cgctgatgcc agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat
    41041 agtgtttacg acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa
    41101 cttcagcttt gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt
    41161 ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt
    41221 ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg
    41281 cgtacatata aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa
    41341 tattcacaat agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca
    41401 atacacgaag aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt
    41461 acaatcaccc tatattccta accataaacc ctaagttgcc tttgttcaca gtaaggtaat
    41521 cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca
    41581 agatatggcg ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccaactgat
    41641 caatcagtta ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc
    41701 tgtactggct gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct
    41761 gatcgagttt atccacacgg cgactctgct gcacgacgac gttgtggatg aatcagatat
    41821 gcgcaggggt aaagctaccg ccaacgccgc atttggcaat gccgccagcg tgctggtagg
    41881 cgattttatt tatacccgcg cgttccagat gatgaccagc ctcggttcac tcaaagtgct
    41941 ggaagtcatg tcagaagccg taaacgtcat cgcagaaggt gaagttctgc aactgatgaa
    42001 tgttaacgat ccggacatca ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc
    42061 gcgtctgttt gaggctgccg cgcagtgttc cggaattctg gcgggctgta cgccggagga
    42121 ggagaaaggc ctgcaggatt atgggcgcta tctcggcact gctttccagt tgatcgacga
    42181 tttactcgat tacaatgccg atggcgaaca gttaggtaaa aacgtcggcg acgatctgaa
    42241 cgaaggtaaa ccgacgctgc cgctgctgca tgcgatgcat catggcacac cagaacaggc
    42301 acagatgatc cgtaccgcca tcgaacaggg taacggtcgc catcttctga aaccggttct
    42361 ggaagcaatg aacgcttgtg gatctcttga atggacgcgt cagcgcgccg aggaagaagc
    42421 agacaaagcc atcgcagcgt tacaggtgct cccggacacc ccttggcgag aagcactcat
    42481 cggcctcgcg cacatcgctg ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag
    42541 tgcgcgggat aattccagta aactccataa gaatacctat tcagcgtaat aatgcactga
    42601 aaaaccagtg catacatctt tatatattct gaatattcac actctttata ggaacttttt
    42661 agagcaatcg gccatcagga gtatagtgat gctcgacaga agaagtgttc tgaatgaaag
    42721 cgactaactt aaggagtgag gaaagatgga aagtaatttc attgactggc atcccgctga
    42781 catcattgcg ggtttgcgca agaagggaac atcaatggcg gcggagtctc gcagaaatgg
    42841 tttgagttcc tcaacgctgg cgaatgcatt atcgcgccca tggccgaaag gagagatgat
    42901 tattgcgaaa gccctgggaa ctgacccctg ggttatctgg ccatcacgct accatgatcc
    42961 gcagacccat gagtttatcg acagaacgca gttgatgcgt agctacacta aaccgaaaaa
    43021 atgagtggtc tggcggtagc cccgcgaacg gggctgccag ctctcagacg attattcgcc
    43081 tttcacacgc tcaatatttg cacctaaagc gcgcagtttg tcttcaatgc gttcgtatcc
    43141 acgatcgatg tgataaatac gatcaaccac cgtcgtccct tccgcaatac agccagccag
    43201 caccaggctt gctgatgcac gcagatcggt tgccataacc tgtgcgccag aaagtttttc
    43261 aacaccgtga caaataacgg tattgctttc gatttcggcg tgcgcgccca tacggctcag
    43321 ctctggcaca tgcataaagc ggttttcaaa gaccgtttcg gtgatgaatc cagtcccttc
    43381 tgccaccagg ttcaacagcg tgaactgggc ctgcatatcg gtcgggaatg ccggatgcgg
    43441 cgcggtacgt acgttaacag ccttcggacg tttgccatgc atatccaggc taatccagtc
    43501 ttcgccgacc tcgatgtccg ctccagcgtc acgcagtttc gccagcacgg cgtcgagagt
    43561 atctggctgc gtgttacggc agataatttt gccgcgagaa atcgccgccg ccaccaggaa
    43621 agtaccggtt tcgatacgat ccggtagcac gcgatagaca ccgccgccta aacgttccac
    43681 accttcgatg acgatacgat cggtgccctg accgctaatt ttcgcaccca gcgtaatcag
    43741 gaagttcgcg gtatcgacga tttccggttc acgcgctgcg ttttcaataa tcgtggtgcc
    43801 ttccgccagg gttgcagcac acatgatggt caccgttgcg ccaacgctga ctttatccat
    43861 cacgatatgt gcgcctttca gacgaccatc gacggaagct ttaacgtaac cttcttccag
    43921 tttgatggtc gcgcctaatt gttcgaggcc agaaatgtgt agatcaaccg gacgcgcacc
    43981 gatcgtacaa ccgccaggta gtgaaacttg cccctgacca aagcgcgcta ccagcggccc
    44041 cagtgcccag atagaagcac gcatggtttt aaccagatcg taaggtgcgc agaatacatt
    44101 aacgtcgcgg gcatcaatat gcacagaacc attacgttct actttcgcac ccagctggct
    44161 tagcagcttc attgatgtat cgacgtcttt cagtttcggg acgttctgga tctctaccgg
    44221 ttcttccgcc agtagagcgg caaaaaggat aggcagagca gcatttttag cgccggaaat
    44281 tgtgacttcg ccctggagct tcgttggccc ctgaacacga aatttatcca tttagtttgt
    44341 tctcagttaa caattcatat ccgctaccgg cgaatcgccc atagctcaaa agccgttcag
    44401 tttgcgatcg cgcgcccact ccgcaggggt atacgctttg atcgacacag catgaatgcg
    44461 gttatccgca atatattcca tcagcggacc atagaccgtc tgctgttttt taacccgact
    44521 catgccgtca aacaactcac ccacggcaat aacctgaaag tggctgccat cgccggaaac
    44581 gtggacttcc tggagggaga gagcgttcat caacacgctc tgaatttcat tattttccat
    44641 gggatcttca atcatcagtt aataaaccag cgaaacatct tagagcaaag ttgcgctggc
    44701 ataaataagc aaaaagcctc gctgataaat cagacaaggc tcgacttgca ggcaggtttg
    44761 ccggacaggc ggttaacgcc atatccggcc tgaaaaaatt taacgaggca gaacatcagc
    44821 aggcaaatta tacaatttcg ccagggtata cactttgtcg tttaccccct gaagcgtcac
    44881 gttgttgccc tgctttttcg ccagatcgat aagatggagc agcagtgcca gtccacccgt
    44941 atccacgcgg gacacacggc taagatcgat gcaggtaatc cccttcaccg cttcctcacg
    45001 catttcccaa agcggtagca gaacgtcctg atccagctct ccggataacg ccagtgtgtc
    45061 acccgtctgc atccagctca gtgactcgct cattattttt tctcttccag agtgattttc
    45121 tgttgagaaa tcgatttcag ttgcgcagtc aggccgtcga tacctttggt acgtagcagc
    45181 gttccccact cgttttgttt ggtggtgatc atactgacgc cttcagcaat catgtcgtaa
    45241 gcctgccagt tgcccgtctg ggagttttta cgccactgga agtccagacg caccggcggg
    45301 cggccattcg ggtcaataat ggtaacgcga ataggcacaa tggttttatc acccagcggc
    45361 tgttctggcg caatctgata ggtttgaccg tgatacatcg ccagcgcctg accgtaagcc
    45421 tgcttcaggt actcacggaa agcggcaaag taagcatcac gttgagctgg ggtcgcactc
    45481 ttgtaatact ggcccagcac cagcgcaccg gcgtatttca cctgtacgta tggcagcagt
    45541 tcctgatcaa caatggtacg cagataatcc gggttggccc gaatttgcgg ttgctcattc
    45601 ttcaggcgat cgaacgtttt ctgcgccgcc tcgtccatca gcttatacgg attggtctgg
    45661 tctgccgcgg ttgccgcact cagcggtgca atcaccagca aagcgaccat cattaaacgt
    45721 ttaaacatgc gtcggttctc ctgaaattat ttcgttgtac ccacaggttc agtggtttca
    45781 ttattacctg gcgcagcagc tggcgcatcg ccactattct tattgtcatc gcctttacta
    45841 ccgtaaagga actgaccaat gagatcttcc agcaccatcg cagacttggt gtcctgaatt
    45901 gtatcgccat ccttcaggat agcagtcccc agttccgggt cttcaaaacc gacgtttaat
    45961 gccagatatt gttcccccag caggccggaa gtacgaatgc tcagcgaact ggtatcagga
    46021 atatggttat aacgttgttc aatttccagc gttacgcgcg gcagataggt tttcgggtcc
    46081 agcgtaatat ccgccacccg gcccacaaca acgccaccaa tactgaccgg agagcgcgct
    46141 ttcaggccgc caatgttatc gaacgtcgca taaagcgtgt aggtcggttc agtacgtatt
    46201 gacgtcacgt tcgccgcctt caggcaaaca aacagcgccg ccagcagtgc tgctaataaa
    46261 aagataccca cccaaatttc attttttttc gtttgcatga actcaattcc caaacatcaa
    46321 tgcggtcagc acaaaatcca gccccagaac agccagagac gagtggacaa cggtgcgagt
    46381 ggttgcccgg ctaatcccgg cagacgtcgg gatggcgtcg tagccgttaa acaacgaaat
    46441 ccacgtcacc gtgatggcga acaccacgct cttaatcaga cagttgacca gatccatacg
    46501 ccagtcgacg gcattttgca ttgccgacca gaagaacccg ctatcaatgc ctttccaact
    46561 gacgccgacc agcgatccgc cccagatccc cacggcgacg aagataaccg tcaacagtgg
    46621 taatgaaata accccagccc agaaacgggg agaaataacc cgacgcagcg gatccaccgc
    46681 catcatctcc atactggaga gttgctctgt agcgcgcatc aggccgattt ctgcggttag
    46741 cgccgaacca gcacgcccgg caaacaacaa cgcggcaacc accggcccca gttcacgcag
    46801 tagcgataac gccaccagca tacccagact ggtttccgca ctataagtgg tcagaaccag
    46861 ataaccttgc agccccaaca ccattccgat gaacacgcca gaaactacaa taatcagcat
    46921 cgacaggacg ccgacattat agagctggcg caccagcagc ggcgcatgtt tgcgaaattc
    46981 cggtttgccg accagcgcat tgaataacat taacccggcc cgcccaaacg ttctcagggt
    47041 tttaatccct ttatgtccga gcgacgccag cgcatttaac agcatgagtg gcttaactcc
    47101 ctggtaaaag atcagcgtga taatcgccgg caggatagcg gaacggaacc ggcccgtcag
    47161 ctatcccgtc cagaaactga cgtacgcgcg gatcaggatt cgcctgcaac gcctgggcac
    47221 tgccatgagc gacaattttt ttgtccgcca ggatccaggc gtgatccgca atacttaaca
    47281 cttccggcac atcgtgagaa accaccacac aagtcacgcc cagcgcgctg ttcagctcag
    47341 aaatcagctt caccagtacg cccatggtga tgggatcttg cccaacaaaa ggttcatcaa
    47401 acatgatgag atccggctcc agcgcaatcg cacgcgccag cgctgcacgc cgcgccatcc
    47461 caccggaaag ttcagaaggc attagtttag ccgctccacg cagccccacg gcctccagct
    47521 tcatcatcac cgtactatgc aacaatggcg cgggaagttg ggtatgttcg cgcagtggat
    47581 aggcgacgtt gtcaaatacg ttcatatcag tgaacaacgc cccggactga aataacatgc
    47641 tcatccgttt gcgcactgta tacaggcgcg aacgagacat cgccggaata ttctcaccat
    47701 cgaaaaggat ctcaccatga tctggtgcga tttgcccgcc aatcagacgg agtagcgtcg
    47761 ttttaccgat gcccgatggc cccatgatcg ccgtgatctt ccctcgcggc acggtcaggg
    47821 aaatattatc gaagatgcag cgattgccac gcgtaaaact gacatcgcgc atatcgacta
    47881 aattcgccac agactgctcc ataattcacc cttcgtcttg cgttgatttt ctaagcatgg
    47941 cgctcaattt aaccttgaac ccaacatatt tacagaatat tacccgccgt ggttagcgaa
    48001 agctggcatt tgttttactt tttagccgca taaagtcaaa attaagaatc cgttacggct
    48061 ttctgaaaat cttcagcgga ccggcgagta tacctgaaga aaggacgtta gatgctttta
    48121 gctacggcac tgttaattgt tggtttactt ttggtcgttt acagtgccga ccgcctggtt
    48181 tttgccgcgt ctattctttg ccgaaccttt ggcatcccgc cgctgatcat cggcatgacg
    48241 gtggtcagta ttggtacatc gttaccagaa atcatcgtct cgcttgccgc gtctctgcac
    48301 gaacaacgcg atttagccgt tggtacagcc ctcggctcaa acattatcaa tatattgctg
    48361 atcctcggtc tggctgcgct ggttcgtcct tttaccgtcc attccgatgt tctacgccgt
    48421 gaattaccct taatgttgtt ggtcagcgtg gtggccggtt ccgtactcta tgacggacaa
    48481 cttagtcgca gcgatggtat ctttctcctc tttctggctg tgctatggct gctgttcatt
    48541 gttaaacttg cacgtcaggc tgaacgtcag gggactgaca gcctgaccag agagcagctt
    48601 gcagagctgc cgcgtgacgg cggattgccc gtcgcgtttt tatggctcgg cattgcgctt
    48661 atcatcatgc cagtggccac gcggatggtg gttgataacg ccacggtgct ggcgaattac
    48721 tttgccatca gcgagttgac gatgggtctg acggcaattg ctatcggaac cagcctgccg
    48781 gaactggcaa ccgcaatagc gggcgttcgc aaaggtgaaa acgacattgc tgtcggaaat
    48841 atcattggcg caaacatttt taatattgtc atcgtgttgg gtttacccgc gctgataacg
    48901 ccaggagaga ttgatccact ggcgtacagt cgtgactaca gcgtgatgtt gctggtgagc
    48961 attatttttg cgttgctgtg ctggcggcgc tccccgcaac cgggccgtgg tgtaggggta
    49021 ttattaactg gcggatttat cgtatggctg gcgatgttgt actggttatc gccaatactc
    49081 gttgaataac tggaaacgca ttatgtcgca cgtagagtta caaccgggtt ttgactttca
    49141 gcaagcaggt aaagaagtcc tggcgattga acgtgaatgc ctggcggagc ttgatcaata
    49201 catcaatcag aatttcacgc ttgcctgtga aaagatgttc tggtgtaaag ggaaagttgt
    49261 cgtcatgggg atggggaaat cggggcacat cgggcgcaaa atggccgcaa cgtttgccag
    49321 caccggtaca ccttcatttt tcgtccatcc tggtgaagcc gcgcatggtg atttaggcat
    49381 ggtcacccca caggatgtgg tgattgctat ctctaactca ggtgaatcca gcgaaatcac
    49441 tgccttaatt ccagtgctta agcgtcttca cataccgtta atctgcatca ccggtcgccc
    49501 ggagagcagc atggcgcgcg ccgcagatgt gcatctgtgt gttaaagtag cgaaagaagc
    49561 ctgtccgtta gggctggcac cgaccagcag caccaccgcc acgctggtta tgggcgatgc
    49621 cctcgctgtc gcgctgttaa aagcacgcgg ctttactgct gaagattttg cgctctcaca
    49681 cccaggcggc gcactgggtc gtaaacttct gctgcgcgta aacgatatta tgcatacggg
    49741 cgatgagatc ccgcatgtta agaaaacggc cagtctgcgt gacgcattgc tggaagttac
    49801 ccgcaaaaat cttggtatga ctgtcatttg cgatgacaat atgatgattg aaggcatctt
    49861 taccgacggt gatttacgcc gtgtcttcga tatgggcgtg gatgttcgtc agttaagtat
    49921 tgccgatgtg atgacgccgg ggggaatacg cgtgcgccct ggcattctgg ccgttgaggc
    49981 actgaactta atgcagtccc gccatatcac ctccgtgatg gttgccgatg gcgaccattt
    50041 actcggtgtg ttacatatgc atgatttact gcgtgcaggc gtagtgtaaa gattcaagga
    50101 taaacaacaa tgagcaaagc aggtgcgtcg cttgcgacct gttacggccc tgtcagcgcc
    50161 gacgttatag caaaagcaga gaacattcgt ctgctaatcc tcgacgttga tggcgtactg
    50221 tcagatggcc tgatttatat gggcaataac ggcgaagagc tgaaagcgtt caatgttcgt
    50281 gacggttatg gcattcgttg tgcgctcacc tctgatattg aagtcgctat cattaccggg
    50341 cgaaaggcta aactggtaga agatcgttgt gccacattgg ggatcactca cttgtatcag
    50401 gggcagtcaa acaaactgat cgcctttagc gatctgctgg aaaaattggc gattgccccg
    50461 gaaaatgtgg cttatgtcgg cgatgatctc atcgattggc cggtaatgga aaaagtgggt
    50521 ttaagcgtcg ccgtggccga tgcgcatcca ctgttgatcc cgcgcgccga ttacgtgacg
    50581 cgcattgctg gcggtcgtgg cgcagtgcga gaagtttgcg acttattact cctggcgcag
    50641 ggcaaactgg atgaagccaa agggcaatcg atatgagtaa agccagacgt tgggttatca
    50701 ttgtgctatc actggcggtt ctggtgatga tcggtattaa tatggccgaa aaagacgata
    50761 ccgcccaggt ggtcgtcaac aacaatgatc ccacctataa aagcgagcat acggacacgc
    50821 tcgtctataa cccagaaggg gcgctaagct atcgattgat tgctcaacac gttgaatatt
    50881 attccgatca ggccgtttcg tggtttacgc agccggtact taccacgttt gataaggata
    50941 aaatcccgac atggtccgta aaagcagata aagccaagct gaccaatgac cggatgctct
    51001 atttatatgg acacgttgaa gtcaacgcac ttgtgccgga ctctcaactt cgcagaatca
    51061 cgacggataa cgcgcagatc aatctggtga cgcaggatgt tacctctgaa gacctcgtca
    51121 cgttatacgg aacaacattt aactccagcg gtctgaaaat gcgcggcaac ttacgcagca
    51181 agaacgccga gctgattgaa aaggttagaa catcctatga aattcaaaac aaacaaactc
    51241 agccttaatc ttgtgcttgc cagctcactt ctggccgcca gcattccggc atttgccgta
    51301 accggagaca ctgatcagcc gatacacatt gaatcggacc agcaatctct tgatatgcaa
    51361 ggcaacgtgg ttacctttac cggtaatgtc atcgtcaccc agggcaccat aaaaattaat
    51421 gccgacaaag tggtcgttac ccgtccgggt ggcgaacaag gtaaagaagt gattgacggc
    51481 tacggtaaac cggcaacgtt ctaccagatg caggacaacg gtaaacccgt tgaaggtcac
    51541 gcttcccaga tgcactacga actggcaaaa gattttgtcg ttctgacggg taatgcttat
    51601 ctgcagcagg tcgatagcaa cattaagggc gataagatca cttacctggt gaaagagcag
    51661 aaaatgcagg ctttcagcga caaaggcaag cgtgtaacca ccgttctggt gccgtcgcag
    51721 ctgcaggaca aaaacaacaa aggccagacc ccggcacaga agaagggtaa ttaattcgtt
    51781 atggcaacat taactgcaaa gaaccttgca aaagcctata aaggccgtcg cgtggtagaa
    51841 gacgtcagcc tgaccgtcaa ctccggggaa attgtcggtc tgctggggcc aaacggtgcc
    51901 ggtaagacca ccactttcta catggttgta ggcattgtgc cgcgcgatgc gggcaacatc
    51961 attattgatg atgacgatat cagtctgcta cctctgcatg cacgcgcgcg ccgcggtatc
    52021 ggctatctgc cacaggaagc ctccattttc cgtcgcctca gcgtttacga taacctgatg
    52081 gcggtactgc aaattcgtga cgacttgtct gctgaacagc gtgaagaccg cgcgaacgag
    52141 ctgatggaag agtttcacat tgagcacctg cgtgacagca tggggcagtc actctccggg
    52201 ggtgaacgtc gccgtgtaga aattgcccgc gcactggctg cgaatccgaa atttattctg
    52261 ctcgacgaac cgtttgccgg ggttgacccg atctcagtta tcgacattaa acgcatcatt
    52321 gagcacctgc gcgacagcgg cctgggcgtg ctgatcactg accacaacgt gcgtgaaaca
    52381 ctggcggttt gtgaacgcgc ttatatcgtc agtcaggggc atttgatcgc ccacggcacg
    52441 cctacagaaa tcttacaaga cgaacacgtt aagcgtgtat accttgggga agacttcaga
    52501 ctctgatagg gtagaagttt gcgacgtttt agcaggagag tacgattctg aacatgaagc
    52561 aaggtttgca actcaggctt agccaacaac tggcgatgac gccacagctc caacaggcaa
    52621 ttcgtctgtt gcagttgtcg acgctggaac ttcagcagga gctacagcag gcgctggaga
    52681 gtaatccgct gcttgagcaa atcgacactc atgaagaaat cgacacccgc gaaacgcaag
    52741 acagtgaaac gctggacacc gccgacgcgc tcgaacaaaa agagatgccg gaagagctgc
    52801 cgctcgatgc cagttgggac accatttaca ccgctggtac accatccggc accagcggtg
    52861 actacattga cgacgagctg ccggtctatc agggcgaaac gacgcagacc ttgcaggatt
    52921 acctgatgtg gcaggtcgag ctgacaccgt tttccgacac tgaccgcgct attgctacct
    52981 ctatcgtcga tgccgttgat gacaccggtt atctgactgt cccgctggaa gatattctcg
    53041 aaagtatggg cgatgaagag atcgacatcg acgaagttga agccgtcctt aagcggatcc
    53101 aacggtttga tccggtcggt gtggcggcaa aagatctgcg tgactgcctg ctgatccaac
    53161 tctcccaatt cgataaaacc acgccgtggc tggaagaggc cagactgatc attagcgatc
    53221 atctcgatct gttagccaat cacgacttcc gcactttaat gcgcgtcacg cgtctgaaag
    53281 aagatgtgct gaaagaagcc gtcaatctga tccagtcgct cgatccgcgc cccgggcaat
    53341 cgatccagac tggcgaacct gagtatgtca ttccagatgt gctggtgcgt aagcataacg
    53401 gtcactggac ggtagaactc aacagtgaca gcattcctcg tctgcaaatc aaccagcact
    53461 acgcctcgat gtgcaataac gcgcgcaatg atggtgacag ccagtttatc cgcagcaatc
    53521 tgcaagatgc caaatggttg atcaagagtc tggaaagccg taacgatacg ctactgcgcg
    53581 tgagtcgctg tatcgttgaa cagcagcaag ccttctttga gcaaggcgaa gaatatatga
    53641 aaccgatggt actggccgat atcgcccagg ctgtcgaaat gcatgaatcg acgatatctc
    53701 gcgtgaccac gcaaaaatac ctgcatagtc cacgaggcat ttttgaactg aagtatttct
    53761 tttccagtca cgtcaatacc gagggcggcg gcgaagcttc ctccacggcg attcgtgcgc
    53821 tggtgaagaa attaatcgcg gcggaaaacc cagcgaaacc gttgagcgac agcaagttaa
    53881 cctctttgct gtcggaacaa ggtatcatgg tggcacgccg cactgttgcg aagtaccgag
    53941 agtctttatc cattccgccg tcaaaccagc gtaaacagct cgtttgaccc aaccgataag
    54001 gaagacacta tgcagctcaa cattaccgga aataacgtcg agatcaccga ggcactgcgc
    54061 gaatttgtta cagccaaatt tgccaaactt gagcaatatt ttgaccgaat caaccaggtc
    54121 tatgttgttc tgaaagtgga gaaagtcacc cacacctcag atgcaacact gcatgtaaac
    54181 ggtggtgaaa ttcatgccag cgcggaaggt caggacatgt acgctgccat tgatggctta
    54241 attgataagc tggcacgcca gttgaccaaa cataaagata aactgaaaca acactaattg
    54301 tccgggcaat tagcatgtgc atggcggcct gttgtgcggc acaacgggcc atttgtacgg
    54361 ttaatgctcc gagcctgttc cactgtttga gtgggcaggt tcttaggtga aattatgaca
    54421 aataatgata caactctaca gcttagcagt gttcttaaca gggaatgtac gcgaagccgc
    54481 gtccactgtc agagcaaaaa acgcgccctg gaaatcatca gcgagctggc ggcgaaacaa
    54541 cttagcctac cgcctcaggt ggtttttgaa gctatcctga cgcgggaaaa aatgggcagt
    54601 accggtatcg gcaatggtat tgccattccg catggcaaac tggaagaaga tactctgcgt
    54661 gccgttggcg ttttcgttca gctcgaaacg cctatagctt tcgatgccat cgacaaccaa
    54721 ccggtggact tactttttgc cctgctggtg ccggcagacc aaactaaaac gcacctgcat
    54781 actctgtcgc tggtggcgaa acgtctggcg gacaaaacca tctgccgccg tttacgcgca
    54841 gcccagagcg atgaagagct gtatcaaatc attacggata ccaaaggtac tccggatgaa
    54901 gcgtagttat tcggtaatgt ctcttttaga cgttgtgagg agaaacagta catggtactg
    54961 atgatcgtca gcggacgttc aggttcaggt aaatctgtcg ccctgcgtgc gctggaagat
    55021 atgggttttt actgcgtgga taaccttccc gtagtgttgt tacccgatct ggctcgaacc
    55081 ctggccgatc gcgagatttc tgccgccgtc agcattgatg ttcgtaatat gccggagtca
    55141 ccagaaatat tcgaacaggc gatgagtaac ctgcctgacg ctttctcacc gcaactactg
    55201 ttcctggatg ccgaccgtaa taccttaatt cgtcgttaca gtgacacgcg ccgactgcat
    55261 ccgctttcca gcaaaaacct gtcgctggaa agtgctatcg acaaagaaag cgatctgctg
    55321 gagcctctgc gttcgcgagc ggatctgatt gtcgatacct cagaaatgtc cgttcacgag
    55381 ctggcagaaa tgctgcgtac ccgtctgctg ggtaaacgtg aacgcgaact gacaatggtc
    55441 tttgagtctt tcggcttcaa acacggtatc cctatcgatg cagattacgt ctttgacgtg
    55501 cgcttcttgc cgaacccgca ctgggatccg aaactgcgtc caatgacagg tcttgataag
    55561 cctgtcgccg cgttcctcga ccgccacaca gaagtacaca attttatcta ccagacgcga
    55621 agctatcttg agctatggtt acctatgctg gaaaccaaca accgtagcta cttgacggtc
    55681 gccattggtt gtaccggcgg gaagcaccgt tcggtgtata ttgcagagca actggcagac
    55741 tacttccgct cgcgcggtaa aaacgtccag tcacgccatc gtacgctgga aaaacgtaaa
    55801 ccatgactgt caagcaaact gttgaaatca caaacaagct gggcatgcat gcccggcctg
    55861 caatgaagct gtttgaatta atgcagggtt ttgacgctga agtgctgtta cgtaatgacg
    55921 aaggcaccga ggctgaagcc aacagcgtta ttgcgctgct gatgttggat tctgccaaag
    55981 gacggcagat tgaagttgaa gcgaccggtc cacaggaaga ggaagcactg gccgccgtta
    56041 tcgccctctt taattctggt tttgatgaag attaatcttc atcactttga catacaactc
    56101 ccttcaaact ccccccgaca ataagaaaat cacgtactga aatcgttctc aatcaacgtc
    56161 aattgtacat ttcgtgcgct ttttactttc agaagaacct taagaaaacc ttaagaagca
    56221 ttgtttaggt ttcgtttaag ttaatcgacc atactggaga tcgtcagaaa atatttccag
    56281 gagatggcat gattcgctta tctgaacaaa gtcccctggg caccggaaga catcggaaat
    56341 gttatgcgca tccggaagat gcccaacgct gtatcaagat tgtctaccat cgtggcgatg
    56401 gtggcgataa agagatccgt cgtgagttaa agtactacgc gcatcttggt cgccggttaa
    56461 aagactggag tggaataccg cgctatcacg gtaccgtcga gacggattgc ggaaccggtt
    56521 atgtttatga tgtgattgct gattttgacg gcaagccctc cattacgctg accgaatttg
    56581 ctgaacaatg ccgttacgaa gaagacgttg cacaactgcg ccaattactg aaacagctga
    56641 agcgttattt gcaggataac cgtatcgtga cgatgtcgct gaagccgcag aatatcctct
    56701 gtcaccgcat tagtgagtca gaggttatcc ccgtcgtctg cgacaacatt ggtgaaagca
    56761 cattgattcc actggcgacc tggtcaaagt ggtgttgtct gcgtaagcaa gagcggttgt
    56821 ggaaacgatt tatcgcccaa ccggcgctgg ctattgccct gcagaaagat ctgcaacctc
    56881 gtgagagcaa aacgctggcc ctcgcttcgc gcgaagctta atccagctgg tgttgttgca
    56941 taaacggctc accgcctaac tgatacatct gccgtaaaat ccacgcctga cggctgcgca
    57001 cgtagcccga tggcgcggag actttgaaac gaagtggatt aggtaataca gctgccagta
    57061 aggcagcttc cgatcgggta agtttgctcg cgggtttgtg gaaataacgt tgtgccgcag
    57121 cttcgacgcc aaacacaccg tcgccaaatt cggcgatatt caggtaaacc gtcaggatac
    57181 gctttttgct ccagacagtt tctatcccca gcgttaatcc ggcttccagc ccttttcgaa
    57241 cccagctacg cccatcccat aaaaagagat ttttggctgt ctgttgagaa atcgttgaag
    57301 caccgcgaat acggttttca ttgcgctcat tgtgcgccag ggctttctca atggaagcga
    57361 catcaaagcc ccagtgctca ggaaatgtct gatcttctgc ggcaatcacc gccagtccca
    57421 tccacggcga gatttgatcc atactgaccc tgtcagaatg tgccacgtaa cgaaaattac
    57481 catgcagcca ggcgctgacc tgtcgctcga ccattaccgc agagaaggga acaggtgcaa
    57541 cgctaaacaa cgcgatgccc ccgccccaga aaacggcgag gacaaccatt aaccgcagaa
    57601 gaaaacggcg aacgaaacta aacaccgtta agcggctttt actcattcag ccagaaccag
    57661 cacgcgggaa accagcttat caatgccgct cgccgcttct gcaatgttct gcgccagcat
    57721 atatgccggg gtggtgacaa tcttattgtc ttcatcaacc acgatatcat cgacaggaca
    57781 cggcacatgc tccgcgccca tctcttccag cacttctgcg gtatcgatat cagtaccgat
    57841 ggtcaaacgc agcgggaaat cgaaaatttt cggcagcatc gccggggcaa tacacataaa
    57901 accaagcggt tttccggcct gatgcatcgc ttgtgccagc gcctttaatt cacggtcaac
    57961 ggtgcattcg cgaccaagac tggcaaaatt gcttaaattc tttgccgcgc caaacccccc
    58021 cggcacaatc aacgcatcca gttcagcggc atcggcctgg gccagaggac ggatttcacc
    58081 gcgcgttatt cgtgccgctt caatcagcac attgcgcgtt tccgtcatcg cttcgccagt
    58141 taaatgattg ataacatcaa cctgctgctt atccggtgca aagcagaccg cctgcgcacc
    58201 gctgcgtgaa atagctaaca acgtcaacac cgcttcatga atttcagaac cgtcatagac
    58261 gccgcatccg ctcagaatta cgccaatttt cttcattatg atcatccttt cgcaattgac
    58321 tgaaacacat taattttttt aataaaaatg gtacgcatca cacatttaac tgattcatgt
    58381 aacaaatcat ttaagttttg ctatcttaac tgcgtgcggc ctgaaaaaca gtgcagtgcc
    58441 cttgtaactc atcataataa tttacggcgc agccaagatt tccctggtgt tggcgcagta
    58501 ttcgcgcacc ccggtctagc cggggtcatt ttttagtggc ttttgccacc cacgctttca
    58561 gcacttctac gtcgtgacgc cactcttctt tcatctcttc aatccattca ccgacgttat
    58621 cttcccaggc gggaaggtca ggagactgaa tttgctgacc cagctgttgc agatggcgca
    58681 accccactga ccccgccgca cctttaattt tatgtccttc ctcaacaatg ccttttttat
    58741 cctgcgccgt cagattcgac tccagcacgt tgacatagcc tggcatcatc ttctcaaaca
    58801 ccgctaaccc gtcggtgatc agcttcggtc ctacaagttc gagatactgt tccagcatgg
    58861 gaatatcgag caatgcttct gatttactgt tctcttctgt cgtcaccgta ctctcctcat
    58921 catcctgggt atcccagaat ttcttgatca tcgcggttag cgccggaaca gaaagcggct
    58981 tactcagcac atcatccatt ccagcattga ggtactcttg tttgtctttc agcacgttag
    59041 cggttaaggc caccagcggc ggtaaatcct ctcgcggata acgcttcgtc agttcacgag
    59101 agatatccag cccggtcata tctggcaact gaatatccag caacaccagg tcgtattcgc
    59161 ccggtttaaa catctccagc gccgccttgc cggtcatggc gacatcaacg ctgttaccta
    59221 atttttccag cacagaacgc gcgacaatca cgttcagttc aatgtcttcc accagcagca
    59281 cattcagcgc cggtaaaggc atatcgtctt catcaaacgc atcatcgacc tcttctgcta
    59341 ccgacggtgc gtggatcgtc aacgtaaagg ttgaaccttt accctgttcg ctggtaaccg
    59401 taatatcgcc gcccatattt ttcgccagac gacgagaaac ggccagacca ataccggtgc
    59461 cggtggcagg tttaccgcca tgactgtctt tcacctggta atacatggcg aagattttat
    59521 ccagctcatc ctgcggaatg ccgataccag aatcttccac ttcaaaatgc agcatatcgc
    59581 cttcatcgta gcgcacgcgc acggtaacct ggccttgctg ggtgaatttg acggcgttac
    59641 tgatgaggtt ccacaggatc tgccgtaaac gcgtcccgtc ggtaatgacc tgatgcggta
    59701 atggcagcgt cggctccagg ttaaagcgca atcctttttg ttgcgcctgc aatgcggaga
    59761 gattttccag atcggcaagg aagctggtga aatcaaccgg ttgattatca agctggacct
    59821 tgcgccgttc catcttatcc atgtcgataa tatcgttaaa gatattcccc agcgtgacgg
    59881 ccgaaacatg gatggtcttg agatattttt cctgctcggc ggtgagttcg gtatccagca
    59941 gaatgcggct caggccgacg ataccgttca gcggtgtacg caattcgtgg ctgatggtgg
    60001 aaataaacgt cgttttgtcg cggctggcgc gttcaagcgc atcctgatac cgcttacgct
    60061 cggtaatgtc gcgaccaaag cccatcaaac cgtgacgttt acccacgcgg tcgtagtacg
    60121 gcactttacg aatttcaaag caggctttgc gtccgtcggg ataatccagc cactgctcat
    60181 aggtcagtga cacattatga cggaacactt tttcatcggt ttcaatgact ttcgcagcgg
    60241 cttccggtga gtaaacatca gcaggtttca ggtgaaccag ttgtttttcg ctttttccgg
    60301 tcagcagctc catcgcgcgg ttacagccgg aaaactcttt atcttcgtta cgataaaaaa
    60361 ccaggtcggg tgaagcatca aggaaggaac gtaaaaatga ggattgctgc tcgagctgaa
    60421 tttgtgtctc ttcgcgctct ttgatttcaa ttttcagttg gccgaaggtt tcctgcagtt
    60481 ctgcttccgc tttttcacga acggcaattt cctgatttag ctgggcaata ttatctttta
    60541 actgaacgtt gaggctcaaa tcgcgctcgc gcatctcctc cagtttttgc accagccgtg
    60601 acagacgttg tcgtgactcc tccagttgct cgacgaccac cgatagaaag tagaccgccc
    60661 acggcgtaat cagcaaacca aagaagatag aacgaataac atcaatgctt tcgacctgac
    60721 catgcagcac catggttacc gccatttgta ccacaatggc aagaacgacg agggccagcg
    60781 ccagcaacat tgagaagcgc accagaccta acttcatcat caggtcaaca taatactgcg
    60841 ccagcagacg aatttgcttc attagggaat tccttcacga caacctgccc caataatacc
    60901 caattctgac agttacgtta taaattgtgt gagaaatgcg gaattcccct caacgaccta
    60961 ctccgttgga ggtagccacg gacgtcccag cgccgatccc tgtacgccat gttcattcag
    61021 atagcgatcc agctcgacca ttcccgtcca tcgattttcg caccacagag gagcaagcag
    61081 cgtcgggcga cgggcgctgg cggaaatgcg gtggtagatc acttccggtg gcgtatggcg
    61141 aatcatttct ccggcagtga gcgtgtaatc ctccagttca ataccgttca aacgccccgc
    61201 ttcccaggct ttcgccataa tgctgccttt cacaatatgc agcggatgca gctttatgcc
    61261 gtccacgccg gtttcaacca ctcgctccag cgtttgcaaa cattcagcct gcccttcgcc
    61321 aggcaggccg acaattaagt gagaacacac cttcagcccg cgctgacgtg ccagctgggt
    61381 tgtacgctga taacaggcaa aatcatggcc gcgattaatg cgatgcaggg ttttgtcgtg
    61441 ggcggtttgt agccccagct ccagccacac ttcgtagccc tggtccttat attcgcaaag
    61501 cagatccagc accgcatccg gcacgcagtc cgggcgagta ccaacacaca aaccgacaat
    61561 attggcctgg ctcaccgcct gctgatacat agaacgcagc acctgaactt ccgcaaaggt
    61621 gctggtatac gcctgaaagt aggccagata gcgtttagcg cggttaacta aattcgcctg
    61681 gtgcgccagt tgctcggcaa tggaacgatg ttgctgcgct tcatcggcaa atgaggcaac
    61741 attacagaat gtgcagccgc cacgcccgat ggtaccgtca cggttagggc agctaaaacc
    61801 gccgtgcagc gtcagcttat gcaccttttg cccataacga cgggtgagat caccaccaaa
    61861 catattgact aatttctgta actgcataat ctgatagacc gcgccttgaa aagaggccaa
    61921 agcctgccat ttttagctca attcggcgat gacttggatc aatcgtcccg gcctgctttt
    61981 atcaactgca taaccaatca aaattaccga aatttcatgc ataatcacat aaatcacttt
    62041 tgcttatctt gtgtcagatt tttttatctc cttatggatt ttaggcaaaa cagtagcatg
    62101 aaacgtcatt accaattaag gcagtataaa atgctggttt tctcgtcagt tcaaggcagg
    62161 ataagggtta acacgccttt atgacagtca ggaattggct gtttctctaa cgacgcccct
    62221 tttagcctta attagaaaat ccattttaat ttcagtcaat taataaagaa ttttgcgcta
    62281 aagcacattt ctgtaccaat aagcttgcca tttgacctgt atcagctttc ccgataagtt
    62341 ggaaatccgc tggaagcttt ctggatgagc agcctgctca tcatatttat gcagtaattg
    62401 agatcccctc ttcaccgtat taaccgatgc gaaaaggaca acaagggggc gaatgcgagg
    62461 cgcgcgtatg acacgcaaac cccgtcgcca cgctctttct gtgcccgtgc gcagcggttc
    62521 ggaagtgggg ttcccgcaga gcctggggga ggttcacgat atgttgtacg ataaatccct
    62581 tgagagggat aactgtggtt tcggcctgat cgcccacata gaaggcgaac ctagccacaa
    62641 ggtagtgcgt actgcaatac acgcactggc ccgcatgcag caccgtggcg cgattctcgc
    62701 cgatggtaaa accggtgacg gttgcggctt gctgttacaa aaaccggatc gcttttttcg
    62761 catcgttgcg caggagcgcg gctggcgttt agcgaaaaac tacgccgtcg ggatgctctt
    62821 cctgaataaa gatcctgaac tcgccgctgc cgcacgccgc atcgttgaag aagagctgca
    62881 acgcgaaacc ttgtcgattg tgggctggcg tgatgtcccc actaacgaag gcgtgctggg
    62941 tgaaatcgcc ctctcctctc tgccacgcat tgagcaaatt tttgtgaacg ccccggcagg
    63001 ctggcgtccg cgcgatatgg agcgccgtct gtttatcgcc cgccgccgca ttgaaaagcg
    63061 tctcgaagcc gacaaagact tctacgtctg tagcctgtcg aatctggtga acatctataa
    63121 aggtctgtgt atgccggcgg atctgccgcg cttttatcta gatcttgcgg acctgcgtct
    63181 ggaatcggcc atttgcctgt tccaccagcg cttctccact aacaccgtac cgcgctggcc
    63241 gctggcgcaa ccgttccgct acctggcgca taacggtgaa atcaacacca tcaccggtaa
    63301 ccgccaatgg gcgcgtgcgc gtacttataa attccagaca ccgcttatcc ctgacctgca
    63361 cgacgccgca ccgttcgtca acgaaatcgg ctctgactcc agttcgatgg ataacatgct
    63421 ggaactgctg ctggcaggcg ggatggatat catccgcgcc atgcgtctat tagtaccacc
    63481 cgcctggcag aacaacccgg atatggaccc ggaactgcgt gccttctttg actttaactc
    63541 catgcatatg gagccgtggg atggcccggc gggcatcgtg atgtctgacg gtcgttttgc
    63601 tgcctgtaac ctcgaccgta acggtctgcg tccggcgcgc tacgtcatca ccaaagataa
    63661 gctcatcacc tgcgcctctg aagtcggtat ctgggattac cagcctgacg aagtggtcga
    63721 aaaaggccgc gtcgggccag gcgaactgat ggtaatcgac actcgcagtg ggcgtattct
    63781 gcactcggca gaaaccgatg acgatctgaa aagccgccac ccttataaag agtggatgga
    63841 gaaaaacgtc cgccgactgg taccgtttga agatctgccc gatgaagaag tgggtagccg
    63901 cgaactggac gacgacacgc ttgccagcta ccagaaacag tttaactaca gcgcggaaga
    63961 gctggactcc gtaattcgcg tactgggcga aaacggtcag gaagcggtcg gttcgatggg
    64021 cgatgatacc ccattcgccg tgctctccag tcagccgcgc attatttacg actacttccg
    64081 ccagcagttt gcccaggtga ctaacccgcc aatcgacccg ctgcgtgaag cgcatgttat
    64141 gtcgctcgcc accagtatcg gtcgtgaaat gaacgtcttt tgcgaagcag agggccaggc
    64201 gcaccgttta agctttaaat cgccgattct gctctactcc gatttcaaac agctcacgac
    64261 gatgaaagag gagcactacc gcgcagatac gctggatatc acctttgacg tcaccaaaac
    64321 cacgctcgaa gcgacggtca aagagctgtg cgacaaagcc gaaaaaatgg tacgtagcgg
    64381 caccgtgctg ctggtgctct ccgaccggaa tatcgctaaa gatcgcctgc cggttccggc
    64441 tccaatggcg gttggcgcga tccagacccg tctggtcgat caaagcctgc gttgcgatgc
    64501 caacatcatc gtcgaaaccg ccagcgcccg cgatccgcac cacttcgccg tgttgctggg
    64561 tttcggcgcg acggctattt atccgtacct tgcctatgaa acgctgggcc gcctggtaga
    64621 cacccacgcg attgccaaag attatcgtac cgtgatgctc aactaccgta acggcatcaa
    64681 caaaggcttg tacaaaatca tgtccaaaat gggcatctcc accatcgcct cttaccgctg
    64741 ctcgaaactg tttgaagcgg tcggtctgca caatgatgta gtgggcctgt gcttccaggg
    64801 ggcggtcagc cgcattgggg gggcgagctt tgaagacttc cagcaggatc tgctgaatct
    64861 ctcgaaacga gcctggctgg cgcgcaagcc catcagccag ggcggcctgc tgaaatacgt
    64921 ccacggcggc gaataccacg cctacaaccc ggacgtggtg cgcacgctgc aacaggcggt
    64981 acagagcggc gagtatagcg actatcagga atacgcgaag ctggttaatg agcgtccggc
    65041 aaccacgctg cgcgatctgc tggcaattac gccgggtgaa aacgcggtca acattgccga
    65101 tgttgaaccg gcaagcgaac tgtttaaacg cttcgatacc gcggcgatgt ctatcggcgc
    65161 gttaagcccg gaagcccacg aggcgctggc ggaagcgatg aacagcatcg gcggaaactc
    65221 gaactccggt gaaggtggtg aagacccggc gcgctacggc accaacaaag tgtcgcgcat
    65281 caagcaggtg gcttccggtc gctttggggt tactccggcg tatctggtca atgccgacgt
    65341 cattcagatt aaagtcgccc agggcgcgaa gccgggcgaa ggcggtcagt tgccgggcga
    65401 caaagtcact ccttacattg ccaaactgcg ctattcggtg ccgggcgtga cgctgatctc
    65461 cccgccgccg caccacgata tctactctat cgaagactta gcgcagctca ttttcgacct
    65521 caagcaggtt aacccgaaag cgatgatctc cgtgaagctg gtttccgaac cgggcgttgg
    65581 caccatcgcc accggtgtgg cgaaagctta tgccgattta atcaccatcg caggctatga
    65641 cggcggcacc ggcgcaagtc cgctttcatc ggtgaaatac gcaggctgtc cgtgggagct
    65701 gggacttgtt gaaacacaac aggcgctggt tgccaacggc ttgcgtcata aaatccgttt
    65761 gcaggtcgat ggcggcctga aaacgggtgt cgatatcatc aaagcggcga ttctcggcgc
    65821 agaaagcttc ggcttcggca ctggcccaat ggtcgcgctc ggctgtaaat atctgcgtat
    65881 ttgccacctg aacaactgcg caacgggtgt ggcaactcag gatgacaaac tgcgtaagaa
    65941 tcactatcac ggcctgccgt tcaaggtgac gaattacttt gagtttatcg cccgtgaaac
    66001 ccgcgagctg atggcacagc ttggcgtaac acgtctggtg gatctgattg gtcgcaccga
    66061 cctgctgaaa gagctggacg gtttcaccgc caaacagcag aaactggcgc tgtcgaagct
    66121 gctggagacc gccgaaccgc atccaggtaa ggcactctac tgcaccgaaa ccaacccgcc
    66181 gtttgataac ggcctgctga acgcgcagtt gctgcaacag gcgaaaccgt ttgtcgatga
    66241 gcgccagagc aaaaccttct ggttcgatat ccgcaacacc gaccgctctg tcggcgcgtc
    66301 gctttcaggc tatatcgccc agacgcacgg cgatcagggg ctggcagccg atccgattaa
    66361 agcgtacttc aacggcaccg caggccagag cttcggcgtg tggaacgcgg gcggcgtgga
    66421 actgtacctg accggtgatg ccaacgacta tgtcggtaaa ggcatggcgg gcggcttaat
    66481 cgccattcgt cctccggttg gttccgcctt ccgcagccat gaagcaagta ttatcggcaa
    66541 cacctgcctg tatggagcga cgggtggtcg tctgtatgcc gcaggccgcg cgggtgaacg
    66601 tttcggcgtg cgtaactccg gtgcaatcac cgtggtagaa ggcattggcg acaacggctg
    66661 tgaatatatg acgggcggta tcgtctgtat tctgggtaaa accggcgtta acttcggtgc
    66721 gggtatgacc ggcggcttcg cttacgttct cgatgaaagc ggcgatttcc gcaaacgcgt
    66781 taacccggaa ctggtggaag tattaagcgt tgacgatttg gcgatccatg aagagcatct
    66841 gcgtggtctt atcaccgagc atgtgcagca taccggttcc cagcgtggtg aagagattct
    66901 ggcgaactgg tcaaccttcg ccactaaatt tgcgctggtt aaaccgaagt ccagtgatgt
    66961 caaagcactg ctgggtcatc gtagtcgtag cgcagcagag ttgcgcgtgc aggcgcagta
    67021 aggggtagca acaatgagtc agaatgttta tcaatttatc gacctgcagc gcgttgatcc
    67081 gccaaagaaa ccgctgaaga tccgcaaaat tgagtttgtt gaaatttacg agccgttttc
    67141 cgaaggccag gccaaagcgc aggctgaccg ctgcctgtcg tgcggcaacc catactgcga
    67201 gtggaaatgc ccggtacaca actacatccc gaactggctg aagctcgcca acgaggggcg
    67261 tatttttgaa gcggcagaac tgtcgcacca gaccaacact ctgccggaag tatgcggacg
    67321 tgtctgcccg caagaccgtc tgtgcgaagg ttcctgcacc ctgaacgatg agtttggcgc
    67381 ggtgaccatc ggcaacattg agcgttatat caatgataaa gcgttcgaaa tgggctggcg
    67441 tccggatatg tccggcgtga aacagaccgg taaaaaagtg gcgattatcg gcgcaggtcc
    67501 ggcaggtctg gcgtgtgcag atgtcctgac gcgcaacggc gtacaagcgg tcgtcttcga
    67561 ccgtcacccg gaaattggcg gcttgctgac cttcggtatt ccggctttca aactggaaaa
    67621 agaggtaatg acgcgccgcc gtgaaatctt caccggcatg ggtattgaat tcaaactcaa
    67681 taccgaagtg ggccgcgacg tacagctgga cgatctgctg agtgattacg atgccgtgtt
    67741 ccttggcgtc gggacttatc agtcaatgcg cggcgggctg gaaaacgaag acgccgatgg
    67801 cgtgtacgca gcgctgccgt tccttatcgc caacaccaaa cagttaatgg gctttggcga
    67861 aactcgtgaa gaaccgttcg tcagcatgga aggcaaacgc gtggtagtac ttggcggtgg
    67921 cgataccgca atggactgcg tgcgcacgtc tgtacgccag ggggcaaagc acgttacctg
    67981 tgcctatcgt cgtgatgaag agaacatgcc gggttcccgc cgcgaagtga aaaacgcgcg
    68041 ggaagaaggt gtagagttca aattcaatgt ccagccactg ggaattgaag tgaacggtaa
    68101 cggcaaagtc agcggcgtaa aaatggtgcg tactgaaatg ggcgaaccgg atgccaaagg
    68161 ccgtcgccgc gcggagatcg tggcaggttc cgaacatatc gttccagcag atgcggtgat
    68221 catggcgttt ggtttccgtc cacacagcat ggaatggctg gcaaaacaca gcgtcgagct
    68281 ggattcgcaa ggccgcatca tcgccccgga aggcagcgac aacgctttcc agaccagcaa
    68341 cccgaaaatc tttgctggcg gcgatatcgt ccgtggttcc gatctggtcg ttaccgctat
    68401 tgccgaaggt cgtaaggcgg cagacggcat tatgaactgg ctggaagttt aagctctgtc
    68461 ccagcgccgc ctcctcagaa aagagtcggc gctgaaattc tcctttcgtt ctggaaagcg
    68521 cctcgcataa tctgaagaaa accaatgtaa tacgcacatc tggattgtta ccggcagatg
    68581 cccggcgtat agctgattcc attattcaac actgcgccat ataaagggaa agataatgga
    68641 atcgctctca gagagaacct caacaggcta ccagcaaatc cacgacggta ttattcacct
    68701 ggtcgatagc gcccggacgg aaacggtacg tagcgttaac gcgctgatga ccgcgacata
    68761 ctgggaaatt ggccgacgaa ttgtcgaatt tgaacaaggt ggcgaggcca gggctgcgta
    68821 tggtgcgcag ctaatcaagc gactatcaaa ggatttaagt ctaaggtata agcgtgggtt
    68881 ctctgcaaaa aacttacgcc aaatgaggct tttttacctc ttttttcaac atgttgaaat
    68941 tcgccagaca gtgtctggcg aattaacacc attgccctgg tccacttacg tccgtttact
    69001 ttccgttaaa aacgctgaca cccgcagctt ttatgaaaaa gagacgctcc gctgtggctg
    69061 gtctgttcgc cagctagaac ggcaaattgc gacccaattt tatgagcgaa cactgctgtc
    69121 acatgacaaa tcagccatgc tgcaacaaca cgctcctgcc gagacgcata ttcttccgca
    69181 acaggcgata cgcgatccct ttgtgctcga atttctggaa ttgaaagatg aatactcaga
    69241 atccgatttt gaggaggcgc tgatcaacca cctgatggat ttcatgctgg aacttgggga
    69301 tgattttgcc tttgttggtc ggcagcgaag gttacgcatt gatgacaact ggtttcgtgt
    69361 cgatctgctg tttttccacc gccgtttacg ctgcctgcta atcgtcgatc taaaagtggg
    69421 caaattcagc tatagcgatg ccggacagat gaatatgtat ctcaactacg ccaaagagca
    69481 ctggacgcta ccggatgaaa atccgcccat cggtctggtt ctctgtgcag agaaaggagt
    69541 cggagaagcg cattatgcac tggctggttt gcctaacacc gttctggcga gtgaatataa
    69601 gatgcaacta cctgatgaaa aacgactcgc ggatgaactc gttcgaacac aggcggtgct
    69661 agaagaaggc tatagactcc gttaatttca gcggtttcga attctgcaga cactgtctgc
    69721 agaatcttta aaacgaacta agttttaagc cattaaatca gccttaacct tcaccaccac
    69781 ctttttaatc tcaccaggtt cgccgacaac gcatcctggt ttatgcggtt ctcccggcat
    69841 aaacacggcg aacattcccg gttttaagtt gatggtttgc tcgttttcaa tggcgctgca
    69901 aagctggtaa tcatcttcat ggtggaactc ttcacactgg cgcgcagtgc ctgccgtgcc
    69961 aaacagaatc cgttcctcac cttttaataa cagctggata tcaatgtatt gctcgtgcaa
    70021 ttccgctttt ttctcgacgg gcgattgagt gttaaacgtc atgacattca taaaaatatt
    70081 gtcgccctgt aattcgtaac gacccggcgc tttttcttgc ggtctggcag ctaatgccag
    70141 cgttaacgcg tcctgtaacg caggatgtaa cccagcagac ggtaatgact gtacttcacc
    70201 catcatcata atttttctcc ctgggccaac agcgcagccc caagtaaacc tgcatcatgg
    70261 cggtagtgcg ccgccagtaa atcaacatga aatgccgctg gctcctgcgc cagatacgtt
    70321 tccaccagcg ccagataccc ttctgccaga ccaacgctgc caccgaccac cacgcactga
    70381 caatcagttg tggctttaat atcagcgatc agccttgcca gcacatgtgc ggagcggtga
    70441 atcagctgct gcgcctgctc gtcgccctgc ccggcgcgca tgaaaatagt tttcgcatcc
    70501 gcgccagcca actccccctg cgctgccgct gcaataccgc gaccagaggc aatcgcttcc
    70561 acgcaacctg tgcgtccaca gccgcagact gggccgtgtg gatcggcaag cgtatgcccg
    70621 atgtgccccg ccagaccgcc agggccggta agcagtttgc cgccgctcac tacaccgccg
    70681 ccgacgccag tggaaacggt gataaacacc atatcggtta tatcgccttc cagcgcctga
    70741 tactctgccc atgctgcggc ctgggcgtcg ttaatggcaa tggtcggcaa atcggtaagt
    70801 tgccccagcg ttttgactaa cggaaagtgt agcaatccac caagattatg cggattcagc
    70861 gccagcaaac tgccgtcgcg aataattccg gttgaagcga tggcaacccg ctgcgcatga
    70921 gcttgcaacg gagagactaa tgcggataag gcatcacgca aggcttctgg tgtctggctg
    70981 gctggcgtag gaagttcacg acgatcgcgg atctgcccgt cagcgccaat cagcgcggcg
    71041 gcaagtttag taccgccgat atcaatcgcc agtgtggtca tagcaccgcc tttttcatcg
    71101 ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat ggcagaaccg accgtcaccg
    71161 cccacgcgcc gtggcgcatc gcatcagccg cctgagcagg cgtgttgtaa cgcccttcgg
    71221 caatcacccg acatccggcg tcgctcaacc ttttcaccag cgtcagatcc ggctcttctg
    71281 gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat aatttcggct cccagctttt
    71341 ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat cgccagtaaa ccgtgatgat
    71401 gaatacgtgc cagcagcgtt tcaacaggca ccggacgcgg gcggtcggtg ccgtcaatgg
    71461 cgataatgtc cgcgcccgcc tgcgccagcg catcgacatc ttcaatatag gccgtgatgc
    71521 gtaccggaga atcctccaga tcgcgtttca caattccaat aatcggcacg ctcaccaccg
    71581 cacgcgtggc ttgcagattt gccacacctt cgatgcgaat ggcaaccgcg cccgcctgct
    71641 ctgccgctaa tgccatggct gcgacgattt cgggtttatc gagcgggctg tccggaaccg
    71701 gctggcagga gacaatcagg ccaccgttag cagcgatttt ttgatccagt tgtgcaagta
    71761 acgacataca tcttccctta gcgaaaggcc cgatacagag accgggcaac aggattaact
    71821 tttggttttg actaaatcgt ttttggcgct gccaaacggc acggcaccgc taaatggttt
    71881 gccgtcgata gcgtcatgag tacgcaacgc ttccgggcgc agccaacgct gaacacgaga
    71941 aggcatatcc agaccaatca gcaggatcac cacaaatgtc agactgaacg agagcgatgc
    72001 cagtgcagta cccagatcca gacgttgagc gatcaacgcg ccgatgattg gggccagtgc
    72061 gccacccaat gcgccaacgt tataggtaaa gcccaggccc gctgcacgct ggtcggtatc
    72121 gaaataaccg ccaatcagtt tcggtaagat cccggcgatc ccttgtccaa gcatttgctg
    72181 gaagaacagt aacagaccga gcacccagac gtttgcaccg ccaatagcaa ataccggaat
    72241 aatcagcagc tgcgaggcca acagactaca aacgtacgct ttgcgggttc ccagccagtc
    72301 accgaggaag ccaccgacgc agcaccccac cgccgcgcca aagccactaa agaacagcac
    72361 attggcgaca gtatgagggt tataagccag atcggttttc agatacgttg gcagcagcgc
    72421 ctgaatcggc catgagtaga ggaaagcaaa caacacgacc accatcagca ttacgccagt
    72481 tggccagcgt ttgcccgtac tctgcaccat aaagctgata aagattgcgg tgcataacag
    72541 cccaagaaca gcgacgatcg cggcattttg caggttaccg gcgaagcaga accacagcgc
    72601 agtagccgcc gccagtgtca ttacgatatt ggcaatgcga tgttcaccac ggtagagaat
    72661 atccaccatt gtgcgtactg gtgctttacc tgcgtgtttc tctttccagt cttctgcttc
    72721 cgggatgttt ttacgcagcc agagagcaaa gatgattggc aaaatgccga taaagaacag
    72781 cgcacgccag ccccagaccg gaaccaccag gctgtagacc tgagcggcaa cgacggcccc
    72841 tacagagaag cctgaaatca gaaagccact ggctttgtta cgcagatgtt ttggccagct
    72901 ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc atccccatgc cgatgaccag
    72961 acgagcgata aacatggtga tgtagcctgg cgcaaagccg catgccagcg tcccggccga
    73021 gaagagaacg atgctggtga ccattgccag acgacgcccg tagcggtcac ccatagcgcc
    73081 gagcatcagg ccgccgaacc agcgagagat aaaggctgcg gagatcagac ttgccgcctg
    73141 caccgtcgtc agcccgaatt caccttgtac ttcggtgagt accagggcga ttaaaacgaa
    73201 atcaaaaccg tcaagcagat atcccaacca ggcagcggaa aatgcccgcc attgtgcacg
    73261 attgagatgg cgataccacg ggatattctg ggttgtagta ctcattgtga gtctcccgcg
    73321 gtgggcgatg cccacacgct ttggtatgaa aattgtaggg tacagatgcg tttatttccc
    73381 ctcacccggt aggggcgagc gaggggaaac aactcacccg cgctcttgca tcaactgctg
    73441 ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggaccaaacg gtttgcggca
    73501 cagcggcaca gaaacgacat ccatataatg cagaacagtt ttcaggccgc ggaatacacc
    73561 cgttttgatc agtaaatcaa tgactttgtt gcattcagtt tgcagtttct gcgcggtctg
    73621 gatatcgcct tctttcagcg ccttaacgat cccctgatag cgccagccca tgatgttgta
    73681 ggtactaccg ataccaccat cagcgcccgc cagcaggcca gaggcgaaga tttcgtcgta
    73741 accgttatag agcaccagat caggatgttc acgacggatc tgctccatct gatagagatc
    73801 gccagaggtc tgtttcagcg cacctacgcc aggcaatgta acaagtgtgt tgatctgatc
    73861 cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg gcaaaccatc
    73921 cgccgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga aaggatagta
    73981 gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg ccgcaagttg
    74041 ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct tacctttcgc
    74101 ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta caaaggcttc
    74161 gccggtcgaa ccacccacgt ataaaccgtc aatgccctgc tgaatattga actgaaccag
    74221 gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca ggagtgcagc
    74281 cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg tctgataaac
    74341 gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac agaaagctta
    74401 taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc tgaccgaagg
    74461 cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg gcttcgtcag
    74521 gatcgtggcg gcggatcgcg tcaacgatcg caatatgctg ttgatagcta acgttgttat
    74581 gctcgtgcag agcctgatca gccacggttg gacgtgcggc gatcagccag tcgagcaggg
    74641 caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg cggtggaaat
    74701 caacgtctga gcgaatgaat gccgcgttgt tatccagcga ctgactgttg atttccagtg
    74761 cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag cgcaccaggc
    74821 tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga tgagaaagga
    74881 aatctttcgc catgccggaa agctcgccga tgatggtgtc cgcagaagga cgtgagacgc
    74941 gggcacgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct gccagcgctt
    75001 cacgcaccga aggacgcccg acgttaaaga acgccatcag ttcgcgttca gacggtaatt
    75061 gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct tccaccattt
    75121 cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt gcaggtgaag
    75181 aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt cagtgtgatt
    75241 aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc agggttttct
    75301 ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa aaattaatgt
    75361 aacgcagata aaaacatccc gtttgaatta tttataagac tattcacgag cattatgaat
    75421 attatgaatg tgttcttaca aaataatcat aagcgcatat ttttttaatg aaaaatcacc
    75481 tcacctacaa ttaaaaacac gacatccgca ccataaatag acttgcaaaa aatataacat
    75541 cattgttttc aatctgccgt ttatgggatt gacccttttc ttttgacacg gagttcaaca
    75601 atgttcggca taattatatc tgtcatcgta ttaattacga tgggctattt gatcctgaaa
    75661 aactacaaac ctcaggtggt gctggctgca ggtatcttcc tgatgatgtg cggtgtctgg
    75721 ttagggttag gtggtgtact cgatcccgcc aaaagcagcg gctacttgat cgtcgatatt
    75781 tataatgaaa tcctgcgcat gctgtccaac cgcattgccg gattggggct gtcgattatg
    75841 gcggtgggcg gttatgcccg ctacatggag cgcacaggag ccagtcgcgc gatggtgagc
    75901 ttgttaagcc gcccgttaaa actcattcgc tcgccgtata ttattctgtc ggcaacttac
    75961 gtcatcggcc aaatcatggc gcagtttatt accagcgcct ccggcctggg tatgttgctg
    76021 atggtcacct tatttccgac actggtgagt ctgggagtaa gccgtctctc tgcggtggcg
    76081 gttatcgcaa ccacaatgtc cattgagtgg gggattctgg aaacgaactc catttttgca
    76141 gcccaggtag cgggaatgaa aattgccact tacttcttcc actaccagct tccggtcgcc
    76201 tcttgcgtca ttatctcggt ggcgatctcc cactttttcg tgcaacgcgc ttttgacaaa
    76261 aaagataaaa atatcaatca cgaacaggca gagcaaaaag ctctcgataa tgtcccgccg
    76321 ctctattacg ccattttacc tgtgatgccg ttaatcctga tgctcggctc gctattcctt
    76381 gcccacatcg ggctgatgca gtcagaactg catctggtgg tggtgatgtt actgagtttg
    76441 acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga cgatgtgcag
    76501 gcgttttttg acggcatggg tacgcagttt gccaacgtgg taacgctggt ggtcgcgggt
    76561 gaaatatttg cgaaaggctt aacgacgatt ggcactgtcg atgcggttat caggggtgcg
    76621 gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctggt cattgccatt
    76681 tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag tcttattccg
    76741 aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat gcattttgcc
    76801 acgacgctag cgcgcggttt cgccgattac tgcggtggtg gtcgttacgt caggaattgc
    76861 aggcgtttcg ccttttgcgg tggtgaagcg gacagcgatc cccatggcag tcggtttcgt
    76921 ggtgaatatg attgccacaa tcacgctatt ttattaagtc attaaaagac aaaacaggcc
    76981 gccagggcct gtttttgtat tacttcacaa cgcgtaatgc cggtcgacca ccgcgtggtg
    77041 gctgcggtgg ttcatcgtca ggatgagtgt catcatcgtg atctggcttg tcgccatcaa
    77101 taaccgacat aacggtttcg ttgtctgccg atgcctcttc atcattcatg atgctggtat
    77161 cttcatcgta ggcagcttca ggctcaaaca tcgtgcctgc gccattttca cgggcgtaga
    77221 tagccagcac ggcagccagc ggcacagaaa cctgacgcgg aatgccacca aagcgcgcgt
    77281 taaagcgcac ctcatcattc gccagttcca gattgccgac agcacgcggc gcaatgttga
    77341 gtacgatttg cccgtcacgc gcatattcca taggaacctg cacgccaggg agcgtcacat
    77401 ccaccaccag gtgcggcgtg agctggttat ccagcaacca ctcatagaat gcacgcagca
    77461 gatagggacg acgtggtgtt agctgtgaca aatccataca gattaactcc ggcccagacg
    77521 catttcacgt tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt
    77581 catatagcct ttcagctctt tcgcacccgg gccgctgaac tcgataccca gttgcggcag
    77641 acgccacagc agcggagcaa ggtagcaatc gaccaggctg aactcatcgc tcaggaagta
    77701 cggcttctga ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc
    77761 ggcatctgct tcagaagctg aaccgttgat gatggtgttc atcagcgtgt accagtcttt
    77821 ttcgatgcga tgcatgtaca aacggctttc accgcgagct accgggtaaa caggcatcag
    77881 tggcggatgc gggaaacgct catccagata ttccataatg atgcgagatt cccacagggt
    77941 cagctcacga tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg
    78001 aggcggattg tccttttcca cgtgttcgat ctcgaaactt acacctttct cagccagcac
    78061 aatgcggacc tgatggctat agatgtcagt aggaccggaa aacagcgtca ttaccgaacg
    78121 tttgttggca gcgacagcca tgaaaacctc caggtatagt cagaattttt actgctacca
    78181 gccaccaggt ggccagtcag aagttgtgtt acccagtaag gaacgactct ctttgttcga
    78241 aaatcaaaca aaaaatgagc aatacccgac atttgggcag aaaattggat gatagtttac
    78301 cagattttgc gacctttgtg gtgagtcgat gccggaaatg gggaaaaaga gatgcgcttt
    78361 agtctgaaat agttgactta gtcccttatt ggcgatgtgg tttttgtttt gcctgtctgt
    78421 cagacggcag caaaaagcaa ctttccattt tttacgctga ttcagaattt aaccataaaa
    78481 aaacccgccg aagcgggttt tttcgcaaat tgttttctgc cggagcagaa gccaattaac
    78541 gtttggagaa ctgcggacga cgacgtgctt tacgcagacc gactttctta cgttcaacct
    78601 gacgagcgtc acgagtaacg aagccagctt tacgcagttc agaacgcagg gactcgtcgt
    78661 attccatcag agcgcgggtg ataccgtgac ggatcgcacc agcctgacca gagataccac
    78721 cacctttaac ggtgatgtac aggtccagtt tctcaaccat atcgaccagt tccagcggct
    78781 gacgaactac catgcgagca gtttcacgac cgaagtactg ttccagagaa cgttggttga
    78841 ttacgatttt accgttgccc ggtttgatga aaacgcgagc tgcggaactt ttgcggcgac
    78901 cagtgccgta gtattgattt tcagccattg cctataatcc cgattagatg tcaagaactt
    78961 gcggttgctg tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt ttacggaaca
    79021 tagcacgacc cagcgggcct tttggcaaca tgcctttaac cgcgatttca atcacacgct
    79081 caggacggcg agcaatcatc tcttcaaagg tcgcttgttt gataccaccg atgtggccgg
    79141 tgtggtgata gtacactttg tcagtacgct tgttgccggt tacagcaact ttgtcagcgt
    79201 tcagaacgat gatgtaatca ccggtatcta cgtgcggagt gtattccgct ttgtgcttac
    79261 cgcgcaggcg acgagccagt tcagtagcca gacggcccag agttttaccg gtcgcgtcaa
    79321 caacatacca gtcgcgtttt acggtttctg gtttagctgt aaaagttttc attaaaagct
    79381 tacccaataa atagttacac gttggtgaac acccaaacgt cttcaattgt tgaggttcac
    79441 acgacaaagt ccggcaaacc taccccttcg aatagcctat gccagcacac aaaaagtttt
    79501 gggaaaaaaa ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga
    79561 tcgacccctt tttgtgattt gtggcaggtt ttaacccgct aaatgctcgc gcttcagata
    79621 ctcttcgctt tgcatctctt gcagacgtga caggcaacgc tggaactcaa acttcagccg
    79681 atcgccctga taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg
    79741 ctcgtaaaac tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt
    79801 cataactggt acatcaaaca acatgaccgt atgaaagaga cgtgagagcg caatatagtc
    79861 atgctgactg cgggcgtcga cgcacagcgt agtaaaagag accgccagcg tctggttctc
    79921 gacgcccatt gtcgctaatg gccgatggtt gatttctaac gtcggtgaat tttctcgttt
    79981 cccccccgcc agcgccaacc atagtttatc catttgcgcc cgggtttcat cgtggagtgg
    80041 cgaaagccac agatgcgcct gagtgagtgt acgcagacga taatcaacac cagcgtccac
    80101 gttcattaca tcacaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg
    80161 caagccattt cgataaagtt cgtccggcgg aatatttgac gtcgcgacca gggtaatacc
    80221 gcgggcgaac agggctttca tcaggccgcc aagtagcatg gcatcggtaa tatcagagac
    80281 aaaaaattcg tcaaaacaga gcacgtcagt ttctgcttta aaacgatcag caataatttc
    80341 cagcggatcg gtctgcccct gtaaggcagt tagctcttca tgcacccgca gcataaaacg
    80401 gtgaaagtgc aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag
    80461 ccaggttttc ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt
    80521 tgtgtcttcc cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg
    80581 ggctggtggc gtgctattga tgagttcctg ataaataatt tccaagcggc tgacggcctc
    80641 tttttgaacg tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt
    80701 tggagtaacg ctttgcatga tcttattgtt attccttgaa taatcggtgc gccgttgttc
    80761 acggttgacg aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct
    80821 gtggaattaa cggttatagt ggcataatca gccgcaggca tggagcctga agccaacacc
    80881 ctacggaaac aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt
    80941 agtcgtcggc atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca
    81001 gcaacaggcg ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga
    81061 agagctggtt agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta
    81121 tcgccagctg tatcagcaca tggcgaaaag ctccagcagc ctgctgccgg aactgtctgc
    81181 tgaagcaaac ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc
    81241 ggtgcagatg cctcgcgact attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa
    81301 gcgcgactaa tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga
    81361 ctatcattta atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt
    81421 tttgaatctc ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaaaccca
    81481 gctgttgagt gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt
    81541 tgcgtcgatc ccaggccagg ttgccgatca ggcccccctc cccagtctgg ctccaatgct
    81601 ggaaaaagtg cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca
    81661 gaaaatcccg gaagaattca aaaagttttt tggtgatgat ttaccggatc aacctgcaca
    81721 acccttcgaa ggtttaggct ccggtgtcat catcaacgcc agtaaaggct atgtgctgac
    81781 caacaaccat gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga
    81841 gtttgatgca aaactgattg gtagcgatga ccagagcgat atcgccctgt tgcaaattca
    81901 aaacccgagc aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga
    81961 ttttgccgtg gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt
    82021 ttccgcatta ggacgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga
    82081 tgcttccatt aaccgcggta actccggcgg tgcactgtta aaccttaacg gtgagttaat
    82141 tggcatcaac actgcaatcc ttgcgcctgg cggcgggagc gtcggaattg gatttgccat
    82201 ccccagtaat atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg
    82261 cggtttgtta ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct
    82321 tgacgtgcag cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcgaaagc
    82381 gggcgtcaaa acgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc
    82441 tgagttgcgc tctcgtatcg cgaccaccga gccgggcacg aaagtgaagc ttggcctgct
    82501 gcgtaacggc aaaccactgg aagtagaagt gacgctcgat accagcacct cttcgtcggc
    82561 cagcgctgaa atgatcacgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa
    82621 agatggcggc aaaggtatta aaatcgatga agttgtcaaa ggaagcccag ctgctcaggc
    82681 tggcttgcaa aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc
    82741 tgaaatgcgt aaagtgctgg cggcaaaacc ggccatcatc gccctgcaaa ttgtacgcgg
    82801 caatgaaagc atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggctgacgtg
    82861 tgatgtccgg ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc
    82921 atgtttgtga agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg
    82981 gttgccatgc cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc
    83041 gatgagacgc ctgccagtta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac
    83101 gtttacaacc gtggtttgaa caccaactct cacaaccagc ttgagatccg caccctggga
    83161 tccggtgtaa tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac
    83221 gccgatcaga tcatcgtcgc cttacaggat ggacgtgtat ttgaagcatt gctggtggga
    83281 tctgactctc taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc
    83341 attccaatta atgcacgtcg cgtaccgcac attggcgacg tggtactggc gatcggtaac
    83401 ccgtacaacc tcgggcagac cattacccag gggattatta gtgctacggg tcgaatcggt
    83461 ctgaacccga ccgggcggca aaacttccta caaaccgatg cttccattaa ccacggtaac
    83521 tctggcggcg cgctggtgaa ctcgctgggc gaactgatgg gcattaacac gctgtcgttt
    83581 gataagagta acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta
    83641 gcaaccaaaa ttatggataa gctgatccgc gatggtcgcg tgatccgcgg ctacattggt
    83701 atcggcggac gcgagatcgc accactgcac gcgcagggcg gtggtataga tcaactgcaa
    83761 gggatcgtgg ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc
    83821 aacgatctga ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat
    83881 caggtggcag aaattcgccc tggttcggtg atcccggttg tagtgatgcg tgatgataag
    83941 cagttaacgc tgcaggtcac cattcaggaa tatccggcaa ccaattaagg ccagcgtgaa
    84001 agacaaaaaa accggagtct gtgctccggt tttttattat ccgctaatca attacttatt
    84061 aacgaactct tcgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc
    84121 gttctgttca aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc
    84181 cagcagaagc ggctgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca
    84241 ttcgacaacg ccttgttcgc cctgcagtgc gcgaaccaga gacaggccaa aacgtgcagc
    84301 agcctggccc atagacaggg ttgccgaccc gccaccggct ttcgcttcaa ccacctcagt
    84361 acccgcgttc tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc
    84421 aggaacctgt gacaacagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac
    84481 ttcaacttcg cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat
    84541 atccagcgtg gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac
    84601 ttcagcagca atcgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt
    84661 cgggcaggtt ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacattaaa
    84721 caggtcggaa cgatccatac ccggtttacg cgctacacct gcagagataa gaacgacatc
    84781 tgcgccttcc agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt
    84841 agggatatgg ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga
    84901 gagttctgaa cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc
    84961 gccagcagcg ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa
    85021 gatatgttgc tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat
    85081 ctcaagaatg tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa
    85141 cctaagttac gtaattaaca atcgcttatg cattagcgca acattccaac aggtggtgac
    85201 aatataccct accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa
    85261 gcagaatttg catgccgtga cgcaggcata tttctcaata acgaaatttg ataaaatccc
    85321 gctctttcat aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc
    85381 tgtatgcaca ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta
    85441 agcaagaaga actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc
    85501 agggcgaaat cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag
    85561 tctcgcggat gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg
    85621 tttactgcct gccagctgaa ctgggtgtac caaccacctc cagtccattg aaaaatctgg
    85681 tgctggatat cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc
    85741 agttaattgc tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg
    85801 ctggcgatga caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtatg
    85861 aagcgatttt agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc
    85921 ggggaaaatg ttgcttatcc ctctcaaccc cctgctttcc cctgctatta atttaacgaa
    85981 tagtgcgttt tactgcgaca tgtcattcac acaatgaata cataaggtaa aaaaagcaca
    86041 ttatgcaaaa ttcattatct aattgaaaaa actagaatta acggtaaata accgtatttt
    86101 taattctttt ttgttattaa aattcacatt tttaacactt agtatcaact gaaacagtta
    86161 gcgcggtatt aattagctca ataattagtg tatacttgat tttgtgatat gggtcacgaa
    86221 acaaaggccc agctaaaaga ttatgtcgag gtaaaaatca tgaaaatcaa aaccactgtt
    86281 gctgcattaa gcgtactttc tgttctctct ttcggtgcat tcgctgccga ctccattgat
    86341 gctgcacaag cacaaaatcg tgaagcaatc gggaccgtat ccgtaagtgg tgtggcgtct
    86401 tcgccaatgg atatgcgtga aatgctgaac aaaaaagcgg aagagaaagg cgcaacggcc
    86461 taccagatta ctgaagctcg tagcggtgac acctggcacg ctacggctga actgtacaaa
    86521 taaaccctca tcgtcttgtc cgacgatatt gcccccggtt cgggggcttt tttttgcgct
    86581 aatgacgaac attaaaacgc aaatgccctt ccagctcttc ctctgcctca tcaaacagca
    86641 atattaacgc gccaaaacgg cgacgcgttt tctctcccag atgaacaaat tcaatctcaa
    86701 gtggtagcgg caggacatcg ttcattaaca catcccatag tgagtcgaga tcgcgtacct
    86761 tatctttcgc cagaccaaag gtttggctaa agtcacgata aaaatcctcc tgactctcaa
    86821 tctcatcaaa atcaaaggta taaatattca tctgttgcca ccgtcacgtt tcgcggcgag
    86881 ttctgccgtg catcgctaag tatagccatg aaaaaaccga cgcttttggc gtcggttttg
    86941 acttaactat cggtcaacgc atgttgatac ttatggagca tccccgccag ccgatgaacc
    87001 ggttccgtca cctgcggtgg cgcttgccag atgcatagct tttcctggta gatttccagt
    87061 tcttccagca actgaccaaa gtaccgacga cgtttatcat cgctacgggc agatatcaca
    87121 tggtctgctg tgcggcgcat ttgtcggtga aacgccgata aatcctcgtt aaccgggatc
    87181 ggcgcatcac gcaggcgctg gtgggcgata atcatcgtca gcgccaggcg aaatttcggc
    87241 aaatcccctg ggaacttatt catcagcaaa aacagctgct gataaagtgc cgggaggtgg
    87301 ttctctttac gacgtgccac attggtagtc atcgcggaaa cagcggcaga aacaaactga
    87361 ttaagcagta cacgtccagt cctgtcgcgc gatttatccc gcaccagcaa aataacggtg
    87421 aacgcgagca cacagccgac gatttgccct aatgcgctgt cgagaaactg actgaaatgg
    87481 aaagtcatcg ggttatccag cacgataata tttatggtgc tggccagcgc ccccatcgag
    87541 cccagtcgcc gtttctgtac ttctattccg aggaagaatc ccagcactgc caggctaata
    87601 cacagcagca acatgctctg ttgggtatta gggataatca ccaaaaagta gagcagccct
    87661 aacggcagcg cggccagcgt cccgtagata aagtcgatcg ccaccatgcg tggattcggc
    87721 aaacgcattg ccagtgacgt cacgaccgca atcatcacca ttgcaccact gccggaagtc
    87781 cagcccgtcc acagccagaa aagcgttccc agaatgcagg aaagtgtggt tcgccagaag
    87841 ttaaccatcg catgatgacg ttcggctgac tctactttga cttccggttc gccttgcagg
    87901 atctcttctt cggtggcgtt gatttttgtg ttactgataa cgccgcgctt gagaagctga
    87961 taacgcgttg ccgccgcgac ccagctataa atggtgacag gcgtttcccg ttccccggtc
    88021 caggcgataa ctcttcgcag gcgtttgagc tgcttgtgga cgtcctgcgc ggtttctacc
    88081 ggcgtgtcaa aaaattcgcg gaaagtatca gtgatcaatt ccgggcgcgt attctgaata
    88141 agataagttt cgcaggattg ggtaatcagc gtcagcgata gcgtattgat cgcttttaaa
    88201 cgtcgattgg cccgcgccca gcgggaagat tccatattca ggttgctgcg catgccttgt
    88261 agcgccgtgg tgcgtcgcac caggtcgccc caggctttat cgacaacttc accatcgcca
    88321 tgcttgatac agagttgcat taattgatat tgcgcgacca gcaaactttc cagctctcga
    88381 tccacttctt gtttgatcga tcgcggagaa aagagcaaat ccgccataat cgcacacaca
    88441 ataccgataa cgatctcgct acaacgttcg acggcaaact gcggcgtaag caatggttcc
    88501 ggctgaatgg tgatcacaat gatcagcgcg gtataaccgg ccagccccca cgcatacgag
    88561 ttttctattc gtaccagcga ggatatccag gtacaaaaac cggcccagat acagcacacc
    88621 agaatcatca ataatggtgc gcggatcatc gcaatgatga tcaccagtcc ggcaatacag
    88681 ccaataaatg tgccgatgat gcgcaaaaag ccacgatagc gaatagcgcc agaatacggt
    88741 tcacctcccg cagcaaaggc cggaccggcg gcaacaatcg ccgctgtcag taccgcccag
    88801 cgtggcgttt ccagctggaa gtgaaagcca acaaacagcg ccagtacaat ggcggtcgcc
    88861 agttttaccg caaagcgaat atgttggtta gcaatggaga aaatatccat cgtgattaac
    88921 caaactcacg caggcgatgg gccattttac ggaagaacga atcctggctt tcgtcgcgat
    88981 cttgtttgcc agtgaccacc actgtagcag tggtgcccgc aggccagatg ttctcttgct
    89041 ggttgtcgag acgaatacga accggaacac gttgcgccaa acgcacccat tcaaggttag
    89101 agtcgatggt cgccatccct ttgtcgtcac gcgtgctgct ggcgttggtg accccagcgg
    89161 caacactatc aacagtccct ttcagcactt tgttactgcc aagcggcgtg atctctgcac
    89221 gatacccagg gcgcacccct tccagcttag tttcttccat ataggccagt acatagaaag
    89281 agttctgttt caccagcgca accgccgttg atccacgagt aataaactca ccggtataga
    89341 cgttgaggtt ggtcacccag ccatctgctg gcgcgcggat caccgtgcgt tcaagatcca
    89401 gttttgccag atcgcgggtc gcctgcgctt tcgctaactg atgcagaacc gtttgtagta
    89461 cgttgttggc ctggtcgatc tcttcgcgag acatcgcctg cacaccgaga cggttacgac
    89521 gtccggcctc ctggcgtttc tcctgtgcca gtacctgata ataagcaaca tcggcttgcg
    89581 cttcctcaag cgccttttga tagcgcggct ggtcgatggt gaacagaacc tgtccttttt
    89641 tcaccagctg gttatgaaca ttcacctggg taatgagtcc agaaacgtcc ggcgcgatcg
    89701 caacgacgtc agcgctaaag cgcgcgtcac gcgtccaggg ggattcggtg taatagaccc
    89761 aggcattaaa aattgcgatg aaggccagaa tgactaatac gaccgtgatg gccgtacggg
    89821 agaattttct tattagtgtt ttcacttcaa cctcaaacga acagtcgcga tatcaaataa
    89881 aacaagcagc aatagagcgc ggtgttgaac aacgccggat gccagacaaa gtcgtagata
    89941 cctgttggca caagtacccg gcacaccagc cagaaaatcg ccagtgataa aagcaattca
    90001 aaaaatatcg gtgggaagga cagcccaaac accacgataa cgggaaacag actcatgttg
    90061 accttggttg tatagagaga gcaggcgtta ttattttcag catctgtcgc cgcagagaag
    90121 ggcatggaaa gccgggcgag agcaacattg ctgtagattg atatttaata tattagcgta
    90181 actgttatgc tgttatctat attatgtgat ctaaatcact tttaagtcag agtgaataat
    90241 ggaacgacta aaacgcatgt cggtgtttgc caaagtagtt gaatttggct cttttaccgc
    90301 cgccgccaga cagctacaga tgagcgtttc gtccatcagt cagacggtgt caaaactgga
    90361 agatgagttg caggtaaagc tgttaaaccg tagcacacgc agcattggcc tgaccgaagc
    90421 cggtagaatt tactaccagg gctgccgtcg tatgcttcat gaagtgcagg atgttcatga
    90481 gcaactgtat gccttcaata acacccccat cgggacgcta cgcattggct gttcttcaac
    90541 tatggcacaa aatgttctcg ccgggctgac agccaaaatg ctgaaagaat acccaggttt
    90601 gagcgtcaat ctggttaccg gaattccagc ccccgacctg attgccgacg gtctggatgt
    90661 ggtgatccgc gtcggcgcgt tgcaggattc cagcctgttt tcccgccgtc ttggcgcgat
    90721 gccaatggtg gtgtgcgccg cgaaaagcta tctcacacaa tacggcatac cggaaaaacc
    90781 cgccgattta agtagccatt catggcttga atacagcgtg cggcccgaca atgaatttga
    90841 actgattgca ccggaaggga tctcgactcg cctgatcccg caagggagat ttgtgactaa
    90901 tgatccgatg acgctggtgc gctggctaac ggcgggtgcc gggatcgcct acgtgccgct
    90961 gatgtgggtg atcaacgaga tcaatcgtgg ggagctggag atcctgctgc cgcgttatca
    91021 gtcagatcca cgcccggttt atgcgttata taccgaaaaa gataagctgc cgctgaaggt
    91081 acaggtcgtg atcaactcgc tgacggatta ttttgttgag gtcggtaaat tgtttcagga
    91141 gatgcacggg cgcgggaaag agaagtaatt catattgtac tgttacgttg tacaaacctg
    91201 tgccaacggg ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgttcgtgc
    91261 acgaggaagg attaattatc cttctgaaaa tactgaaatt attacgcagt accgccaaca
    91321 gtcaggttat caactttcag cgttggctgg cccacgccaa ccggcaaact ttgcccttct
    91381 ttaccgcaga cacccacgcc gttatccagt ttcaggtcgt tgccaaccat cgaaatctgc
    91441 tgcatggttt cgataccgga accaatcaac gttgcgcctt tcaccggctt cgttacttta
    91501 ccgttttcaa tcagatatgc ttctgaagtg gagaaaacga atttgccgga ggtgatatcc
    91561 acctgaccgc caccaaagtt cggtgcatag ataccgtact caacggattc aataatttcc
    91621 tgcggggtcg atttacccgg cagcatatag gtgttggtca tacgcggcat gggcagatgg
    91681 gcgtaggatt cacggcgacc gttgccagtc ggcgtcatcc ccatcaaacg cgcgttgagt
    91741 ttatcctgca tgtagccttt cagaatgccg ttctcaatca gcacgttgta ctggcctggc
    91801 gtaccttcgt catcaatcgc caccgaacca cggcgatcga ccatcgtacc gtcatcaacc
    91861 acggtacaca gttctgaagc caccagctcc ccgacctgtc cactaaatac tgaagtgcca
    91921 cggcggttga agtcgccttc cagaccgtga cctaccgctt catgcaacag cacgcccggc
    91981 caacctgcgc caagtactac cggcatggtg cccgctggtg cagcaacggc agaaagattg
    92041 accagcgcca tacgtactgc ttcttttgcc catgcatccg cacggacttc gccgtcgaga
    92101 tcggcaagga agaactcata accaaaacga ccgccgccgc cactggcacc gcgttcgcgt
    92161 ttgccatctt cttcgatcag aacgctcacg gaaagacgca ccagcggacg gacatccgcc
    92221 gctagcgtgc cgtcggtggc cgcaaccaga attaattcat agacaccact gaggctggca
    92281 gtcacttcct gtacgcgctt gtccgcttcg cgggcaacct tatcgacgcg acgcaggata
    92341 tccagcttct cttcacggct catgctttgc agcggatcta ccgaggtata caacgggcta
    92401 tgctctaccg cgcccagcgt ctgtactttg ccatcaccgc tatcacgggc gatggtgcgc
    92461 gccgcttgcg cactctgttc cagcgccagc aggctgattt ggtcagcgta agcaaatccg
    92521 gttttttcac cgctgattgc acgcacacca acgccctgat cgatgttgta agaaccatct
    92581 ttaataatgc ggtcttctaa aacccaggat tcgtgatagc tcgactgaaa atagagatcg
    92641 ccataatcaa ggcgacgttc ggccagttga ccgaggatca cgaacaagtc ctgatgtttc
    92701 aggccgttcg ccgctagcaa ttgttcactt accaggttaa gactcatcgt ttttgctact
    92761 cgttagttac tgcagtagag gattttttac ggctgccgga tgcggcgtga acgccctatc
    92821 cagcctacgg ttatgttctg gctcgttggc ctttggcaac gattatccta tgagattggg
    92881 gcaattacgc gccctcgtca aatcattgcg ctttttcttt acgcggttgg cgcaacactt
    92941 cgttgatttg cggatcgtcc agcggacccg aaatgtgata gcgcaaaatg gagactttgc
    93001 tccacagcgg ccccagcact ttactggcgg caaacactgc cgcgccaaca atggggttaa
    93061 ccgcaaaagc cgcagccacg cccactgtcg cagaaatctc tggtgcgaca accgcttcca
    93121 tattcaggtc gcgacgtacc agatttaccg accctttcat ggcgatatcc gcctccaggc
    93181 catccaccag cgtgtcgtcg gtgtgcataa cgccgtcttt aatccacgcg gtgctgcgaa
    93241 tggagtcaaa atagaaccct tcgccaaaag tgtctctgaa atcaaaacgc agcttacgca
    93301 tcagggcatc tacgctcaat aagcgcagca attgcccggc atgtccggta ttgatttcgg
    93361 taatttcgcc tttacccagt tgagtatgaa tgatgccatt caacgtcgcc tcatctggct
    93421 gccacggtgc tttacgccag tgtaaatcgt aatccacatt aaatgacgac tgccgtatgg
    93481 gcgtcgtgac accaaaaaat tctgcggcgg catcaatttt ctgcccgcgc agttttcctt
    93541 tcagcgaggt acgttcattc cccggattgt taatccattc accatcggca gtgagccgcg
    93601 aaaaaccagt atcaatcagt ccattggtca gcgttaatgt attgccagaa atggtgatat
    93661 cactgtcaat gcgcccgaat ttttgccccc agaaccagca ctctgcgcat cgtatttggg
    93721 cgtccgacca gccacggaag ttaatgcgtt ccgttgtcgg gaaaggtgat gacggcgttg
    93781 aatcaccacg agttttcgcc acgctcgggt tgtaataaag atatttgata ttcgccagcc
    93841 acggcgcgtt attacgcatc gccagcgtgg cgttgatttc acgcccctgc gcctcaacct
    93901 gggtgccatt tgccgtcggt tgcgaaacaa tactcaggtt attccattgc tgatttccca
    93961 gcgacaacat aggcgtacgt aacgttatgt gttgtgggaa actcgctgca ccaccgacac
    94021 tctccgccgc acctttctga aacagggcca gccactcggc accattcatc ggcggcatat
    94081 tgagttcaac accactttgt tccggcaacg gcgggagcgt tttactgtct gccgcccaaa
    94141 tagcacggtc gagcgtcagc ttttgaccga gcaaccagcg gctattgaaa tgattatccg
    94201 caccagcctg tccggttaat tcaaagctgt tgagattgcc atcaacctta acgtttaccg
    94261 gcagtggttc acccgcaggt ttggctaacg gtgaaggtaa gtgactgctc acattcttca
    94321 aatcgccgtt tagctctacg ttatacgtcg cgccagcatg ataaggcaga acaatgccca
    94381 ctttaccatc ccacgccacg ctgccactca atgcttcgtt caccgctgca ggcagaacgc
    94441 cggttttcgc cggttgccag ttaccgttga gattcaccgc gacctggtag gcttttgccc
    94501 cttctttggt ggaaaaatcc acgttcaacg gctgattaaa ccagcttgct gtcagtggtt
    94561 cactttgcag atcgccattg ataaagctga atttaccgct caaatttttc agggtgctgt
    94621 cgagtggttt gataaacaga ctgttattac gcagcgtcac ttcacctttc gcggttacca
    94681 gctcgccgtt cagcgggata tcaagatgta agcgagcatt cacatcgccg tcgagctgga
    94741 gttcttgcag ggtcgcaccc agagaatctt tcagcggtgt ctcatcaaag taagggccaa
    94801 cggctttacc cggaccttta atgtcagcgt caatcagcag tttttctttt gagtagtcag
    94861 ggatcactgc ggtgagatta ctcgcgcgca cgccgcccag attaacgcca tcggttttca
    94921 tccataaacc gtcgttaata aagtccagtt caataccaag gttagttaat gcaggccagt
    94981 ccggctggaa ggcaaacttc gcgttgcgca gcggcaccag cacttcaaac tgaccttcgt
    95041 tgtgtttata ggggaagagt tgcggattgc caccataaac cagcgtcgcg ttatccgctt
    95101 caccgccctg aatcgcgcca cttaagtaat caaccaggtc tttacccatc aagttttccg
    95161 ggaaatagcg ccaggcttgt gaaccatcat cggtactgat gccagccaga atacccagcc
    95221 agggttcatc gttagcaggt tgcaggtaac gaaaaccgcc gcgcgcatgg acggctttgg
    95281 ctttaacgtc aatattacgc ccatccagct ggaaaccttt gtcattgttc agccagctta
    95341 tagttgcctg gccgtcggcg atttccagtg gcgcacggaa taccgtttcg taaggcatct
    95401 ttgcctgctt catcgacgcg gtaagcaaac cattttcaac gctgccggaa agcgtcccgg
    95461 agaagtgttc cgcacccggt aataatttcc attgcttcca ggccagatcg ctccacgatg
    95521 cctgaaaacg ggtcttgtct gccgcctgaa gcgggatatc cagcgccaga gtgttaatct
    95581 tgccgctcgg ttgtgtagag cgccaaacat cacccagtgc aggtgaaagt ttcgcggcca
    95641 gcgggcgtat gccctccagg cctgccagct ccagattact ggcgcgaatc cggagttcgt
    95701 cactgcgttt attgtctttg ccgccaacgt cctgttccgg tatccaggcc agcgtcaatg
    95761 ctccgctcgg ccagggtttg ccgtccatcg tgatccgtgt atcgggaata gagaactgcc
    95821 atcccggatt ttcacgcgta atatgcgcgg tcagattatc caccgacagc gtatgcgttt
    95881 ccttctcgcc caaccagctg gcaccaccct gtttcagcca gacgtcaccg ccggttacat
    95941 cgcctttgtc gatcgtcatc cagccttcaa gggagaactg cgccgtttcc agcgcaatat
    96001 tgtcctgcat ccatttaccg agccacggct tcaggtcgat gtcatccgcc tggagccaga
    96061 cgcgaccatt gcttaacaac ccctcatcat cgcgcaaatc catgcgcacc tgcatcacgc
    96121 cgtgctgtcc ggtaaggctg gagaggctta ccaggccttc cgcacggtgt cgacgtggat
    96181 cgttcagcca ggtgagttgt gggatcgcca gctcggcgcg ctgaccggat ggcgtcagga
    96241 aactgacttc actgtcgcgg agatcgaaat ggtcaaattg acgaagaaac agatcgctga
    96301 tgtgactggc ttccagactg tcatcgctac caccgctggt gataggagtg ttggtgcgaa
    96361 agcgcagctg ccagaaagtg aggtcgcgaa actgccagcg catatgtaac aggctctgcc
    96421 agacatccag cgccagagta acgcgtttaa ccgaaaattc gccgccatcc tttagttctg
    96481 cacggatgtc gtgcgcttca agcgtcgggc caaaattctg ccagctggct gagagctgac
    96541 tggcttctac cggcatgcca gtcgcggatt ctattttgtt gaggatttcc ggacgccagg
    96601 cgtcaagatg cggtaaagca atacgcaggc cgctaaccag cagggcagcg atcacaacga
    96661 gcgcggctcc agtaagcagt aaaatccccg gcaatcgcct cacccgtcac tccttgtctg
    96721 ctaaaaatgt gactcaaaaa ccctttgccg gatggcggcc cagcatctgt ttacatcatt
    96781 acgacgtcaa actgctcctg gttatagagc ggttcaattt gtactttaac ctgtttgcca
    96841 acgaaaattt ccacttccgc cagcgagtgt gactcttcgc ctttcaaggc ttcggctact
    96901 gccggagaag catagaccag gaaacggtcg gagtcgtaag catggtgtac acgaacaatc
    96961 tcgcgcatga tttcatagca taccgtttcc acggttttca ccgttccgcg accgtggcag
    97021 gttgggcatt cgttacacag tacgtgctca atgctttcgc gggtgcgttt acgcgtcatc
    97081 tccaccagcc ccagcgccga aaaaccatta acgctggttt tcacccggtc tttgctcaac
    97141 gcctgctcca gcgagtgcag cactcggcgg cggtgatctt cattattcat atcgatgaaa
    97201 tcaataatga taatcccgcc cagattacgc aaccgtaact ggcgagcgat agcctgcgtc
    97261 gcttcaatat tggtattgaa aatggtgtcg tccagattgc gatgaccgac aaacgctccg
    97321 gtattgatgt ccacggtggt catcgcttcg gtctggtcga taatcagata accaccggat
    97381 ttcagttcaa ctttgcgttc cagcgctcgc tggatttcgt tttcgacatc aaagagatcg
    97441 aaaatcggct ggcgtccggt gtaatgctcc agcttgctgg tcatctcggg aatgtactcc
    97501 gaggtgaact caagtaacgc ttcgtaagtc aggcgtgagt caacgcgaat gcggtccagt
    97561 tcggcatcgg cgaaatcacg cagaacacgc tgcgccaacg ccagttcgcc gtacagctga
    97621 taacgggtct gcgggcgctt tttacgctcc ataactttgg tccagacgcg tttcagataa
    97681 gcggcatcgg aggccagttc agcctcgcca accccttccg ctgcggtacg gatgataaac
    97741 ccgccctgct cgtcgcaata ctctgcgacc acttttttca ggcgttcacg ttctgattcg
    97801 ctttcaattc gttgggaaac cccaacgtga gaagcccctg gcataaagac cagatagcga
    97861 gaaggcagcg tgatatcggt ggtcaggcgc gcccccttag tgccaagcgg atctttcacc
    97921 acctgcacca tcagatcttg cccctgacga accagttccg agatgtcgcg caccgtgaat
    97981 tgcttttgtt cttcacccgc cacacattcg gtgtgcggca tgatgtcaga tgcatgaaga
    98041 aacgcggctt tatccagccc aatatctaca aaagccgcct gcatacccgg aagtacacga
    98101 cttacacgac ccttgtagat attgcctact attccgcgtc gcgcctcacg ttcaatatga
    98161 atttcctgca gaataccgcc atcaatatac gccactcgcg tttccgaagg cgttacgttt
    98221 actaacaatt cagccgtcat gtttatccct tttctcacgc agtgcgttaa aattacttaa
    98281 taattcatac gtttcaacca gcggtaagcc gactacggct tgatagctgc catttatctt
    98341 cctgacaaaa cagccaccca gcccctgaat accgtatgca cctgctttat ctaacggttc
    98401 accgctggcg acatagcccg cgatgtcttc gtctgttaac gttctgaaag tcacatcggt
    98461 gaccaccagg caatcgagaa tgtgccggct gtcggccaac gccactgccg tcatcacctg
    98521 atgggtctga cccgataatt tgcgcaacat ctgcgccgca tgctctgcgt cgcgcggttt
    98581 ctccagcact tctccgttca ggataacgat agtatccgca cccagcaccg ggagatcctt
    98641 cgccgtttgc gcgacacctg cccgtgcttt ctcgcgcgcc agacgcacaa catactgctg
    98701 cgcgctctcc tgcggctgac gctgctcctc aatgcccgta acaatacgtt caaaggtcac
    98761 gccaagttgc gcaagtaact cctgacgacg cggagaaccg gaagctaaat acagagaagt
    98821 catagaaacc tttattgcac tgcaaactgc tgacggactt tacgcatcag caagaaaatc
    98881 cacggccaga gcaccccatt gactacacta ctccagaaca cttccggtct gaaagagacg
    98941 ttaatcacta aaaactctgc ccagaaaaca ataatatcca ccaccagcga aagcaacatg
    99001 acgaccagcg cctgctgcca taatgcgagg ttgcggaaaa gctggtattt cagcgccacc
    99061 aggtaagcaa tgatgctcat cgccaatacg cgtacgccaa gcgtcgagcc gctgatcaga
    99121 tccagtatgg cacccatcac aaaacctgtg cccacattta cgcgatgggg caaggccagg
    99181 atccaataca acaagatgag taacacccag tttggccgga aaacaatcag gttatccggc
    99241 cagggcatga tttgcagcaa aagcgcaatg aggaaagaga gccagattac ccagcgtccc
    99301 tggctacgat agctcgccac tattgccctc ccggcgcacg cgcaggcggt tgagcaccac
    99361 tttgcggcgg cgtagccctt tgtagagagc gattagcagc gggctgtgtg ggcgcagcag
    99421 gcgcagtagc tgcatttcct gtcgtgggtt gctgcggagt cggctgagcg atccccgttg
    99481 ccggttcagg taactttggc cccatcgcgt ctggcgaagg caatacctgc ggcatcatct
    99541 gcatcagacg ttcattagca acacgatgca cctcttccgg cgtcatcggg ttagcgccgt
    99601 tacgatctgc cccccacagc agcagcagat aacgcaaacg ttgcagccct gcagtcggac
    99661 gcgcctgaat cacagtataa gcgcgctggg tatcgagttt tacggaagag acaaccgcga
    99721 ccggatagcc ttccgggaaa cgaccgccca gaccggaggt caccagcaca tcaccaacac
    99781 gaatatccgt attcgctggc agatgctcaa gctgcaaatc atccgtacaa ccgttaccgg
    99841 ctgcaattac gcggatatcg ttgcgcagca cctggattgg cagcgcgtgg gtcgcatcac
    99901 aaatcagcag cacgcgactg gtcagtttag cgacggccac cacctgacca acaacacctt
    99961 tgtcgctgat gaccggctgg ccttcataaa cgccattaac gctaccttta tcgataacaa
   100021 cttgatcgct ataaggatcg ttaaccgtgg agataacctg agtcaccatt ttctgctcat
   100081 cctgacgcag cggggaaccc agcagctcgc gcagacgcgc gttctcctgt ttgtattgtc
   100141 caagcatcag cagttcactg tttttcagca acagttcctg acgtaacgcc cgattttcaa
   100201 gttctagttg gtcacgcgag gccagcgtct gcgatacgcc atccagcaat tcacgaggag
   100261 cattggaaac aaagtagaaa ggactgacgg cggtatccat ataagtacgg atttgactga
   100321 acgtccccag gcggctgtcg gcaataataa tgccgagcgc caccagcacc gccagaataa
   100381 ggcgaatctg tagcgacggg ccacggctaa aaattggctt cataagttat gcgtattctc
   100441 gtatcagacc aggcagggta aacagacgct tcccctgcct gcatccgatt actcttcgct
   100501 gaacaggtcg ccgccgtgca tgtcgatcat ttccagcgct ttgccgccac cgcgcgccac
   100561 acaggtcagc gggtcttcag caacaacgac tggaatgccg gtttcttcca ttaacaaacg
   100621 gtcaaggtta cgcagcagtg cgccaccacc ggtgagtacc atgccgcgct cggagatgtc
   100681 ggaagccagt tccggcgggc actgttccag tgcaaccatt accgcgctca caataccggt
   100741 cagcggttcc tgcagtgctt cgaggatttc gttggagttc agggtaaaac cgcgtggaac
   100801 accttctgcc aggttacggc cacgaacttc gatttcacgg acttcatcgc ccggataagc
   100861 cgaaccgatt tcgtgcttga tacgttctgc ggtggcttca ccgatcagag aaccgtaatt
   100921 acgacgcaca tagttgatga tagcttcgtc gaaacggtca ccaccaatgc gtacggaaga
   100981 ggagtaaacc acgccgttca aggagataac agcaacttca gtggtaccac caccgatatc
   101041 aaccaccata gaaccggtcg cttcagaaac cggcaggcca gcaccaattg cggcagccat
   101101 cggttcttca atcaggaaga cttcacgggc accagcgccc tgcgcggatt cacgaattgc
   101161 gcggcgttca acctgggtcg cgccaaccgg tacacaaacc agaacgcgcg ggcttggacg
   101221 cataaagctg ttgctgtgca cttgtttgat gaagtgctgg agcatttttt cagtcacgaa
   101281 gaagtcggcg ataacgccgt ctttcattgg gcgaatggca gcaatattgc ccggcgtacg
   101341 gcccagcatc tgcttcgcgt catgacctac tgcagctacg cttttcggtg aaccggcacg
   101401 atcctgacga atggccacca cggaaggctc attcaatacg atgccttgtc cttttacata
   101461 aatgagggta ttcgcagtac ccaggtcaat ggacaagtca ttggaaaaca tgccacgaaa
   101521 ttttttcaac atactaaggg ataatcctga aagctggggc ggaaaagaaa atccgcttac
   101581 tttaccaacc acacgcagca gcgacaaggc gcaaaaatca tctgctacgg tgaaaattag
   101641 tgcagttcgt ttcctttgtt acaaatctct gcctgagtcc agaaaggctt aatgcatcag
   101701 cagcattcct cgcctgtttg caaccgcgta aggtcattca tctgcatatg tgctgcaaca
   101761 atctggcgag cagacaagca cactcccatg agacgcagcg cgcgttattc tacgtgaaaa
   101821 cggattaaac ggcaggttaa accgagtatc tttgtgaata ttttttcacg ttagtatcaa
   101881 gtggctgtga ggacgcgaaa aaatcccctt gcccgcctgt aacaccgcgc tgaatcaggg
   101941 tctgccactc gcttcgcgaa cgcacaccgg tggcgtaaac ctgggtgctg gtccctgagc
   102001 aggcttccac caggctttga accagcagct ggttctccgt tcgcttctca atgtttctga
   102061 ccagccccgg atggagcttg agtaactcaa cattaagttc tttgatccaa ctggtgctta
   102121 ccagcgtcaa accagcctgg ttgacggcta cccgtacccc taaagcattc actaaacgaa
   102181 taacaggttg taaacgactg atatgttgac ctacatcggc ctctgcaagt tcaataatta
   102241 tgcgttttcg ttgtgatttt tcacattgca ttaacgtatc gcgcagccaa cgctgaaaac
   102301 gcgggcgaat cagcgactca acggtaacct gaatcgccag attttcctct ggccagtaac
   102361 gcaatagtgg aataagacgg ctgatttgca gacggtcata ctcttccgat aagccaaact
   102421 gcaagaccat cggcatatac tccgccgagc taacctcttc attaccatca aagatgcggc
   102481 acatgagttc gcgatgatga acctgacctt cgcgagtaac cgccggtttt tgataaaggc
   102541 gcgggccgcc gcgactgagc atttgctcga taagcgtacg ccagcgaaca ttaccgcgtc
   102601 ctttttcagg caacgagtca tcgtaaatag cccagctatt gccgccctgc aatcccgcat
   102661 tacgcgtggc agactctgca tgttccatta cctgctcggt atcctgacca ctacgccagg
   102721 cgcagatacc aatgtggatc atatcgtcgc gatcgagcat tttattgttc ggcaaggtat
   102781 cgacggcttt gattaactga ccggcgatgc tctctgcctc ttttaacgtc cggtgcggta
   102841 acagcgcagc aaaatcactg cggtggtaac gcgccagcag tgcgccaggg tagcgcatca
   102901 taaatgtcga cagcagattc gtcagagtga agaactgttc ttcaacctgg ctgtgccccc
   102961 aggtatcgct caacatattg aaatccggca gacgaatcat catcacgatc ccgtgggtac
   103021 ctactttctc ctgatcttcc agtaacgttg ctaactgatt atcgaaaaag agtcggttat
   103081 tgaggccggt tttcacgtcc tgtgcggcat aagagcggat cagcgtatca agacggctgt
   103141 gttgttcgcg tgcgttctga atttcacgaa gcagcgtatc cagcgcactg ctggttctgg
   103201 gcggccattc atagatggtt cccaacacat tagagccacg ctcaccgttt aagatacgag
   103261 tagcccgggt ttccagcaat tcttgcccgg caagttgccg ttgtaaccag cgtaccgcca
   103321 gaaagagcat aagaatgata aagccaatcg cccctgtgag cggcgcggtg gtcatcaacg
   103381 aatggaaata gttgcccatc ggatcctgat aaaccagacg caacgacatc cccggatgct
   103441 ttatcaacgg aacgctcagt tcacgaaaca gatcgctggt gccaaccgga cgataactac
   103501 catttctggc cagggtataa acctgcttat caccatggag caggtctaca cgaacgatat
   103561 ctgccgacat cattaattcg gtaatttgtg gccttaatgc gctgaagtca ttcgacacaa
   103621 ggtgggtatc aatcgccgtc gccaccgcct gaacgcgatg actaaactta tactgaatgg
   103681 cgttgtagaa acttagcgaa cagcccagca aagtcacaaa aattgttaac ccggtgagca
   103741 gcgtaacaaa ggccgaaaat ttcgtcgtta atctcatcct tgtgttaact ccgatagtga
   103801 ggaagcgggc atactagcaa atcagattta tctcgcaatt tattgcgctt catcggcttt
   103861 gcttttccat tagcgagtat agtcttcaga aattattttc caatccatca tgcacatgag
   103921 gaccacttat gcaggcgtta cttttagaac agcaggacgg caaaactctc gcatcagtac
   103981 agactctgga cgaaagtcgc ctgccggagg gcgatgtcac ggtcgatgtt cactggtcga
   104041 gcctgaacta taaagatgcg ctggcgatta ccggtaaggg aaaaatcatc cgtaattttc
   104101 cgatgattcc tgggatcgat tttgccggaa ctgtacgcac cagcgaagat ccgcgttttc
   104161 atgccggtca ggaggtgtta ctcactggct ggggcgttgg tgaaaaccac tggggtgggc
   104221 tggcggagca ggcgcgagtg aaaggtgact ggctggttgc catgccgcaa gggctggacg
   104281 cgcgtaaagc aatgattatc ggtactgccg gttttaccgc catgctgtgt gtgatggcgc
   104341 tggaagatgc cggtgttcgc ccgcaggacg gggagattgt cgtgacgggt gccagtggtg
   104401 gcgtcggcag taccgccgtg gcgctgctgc ataagttggg ttatcaggtc gttgccgttt
   104461 ccggtcgcga aagtacccat gaatatctga aaagtttagg tgccagccgt attctccctc
   104521 gtgatgagtt tgccgaatcc cgtcctctgg aaaaacaagt ctgggctggg gcaattgaca
   104581 ctgttggcga caaagtgctg gcaaaagtgc tggcgcaaat gaattacggc ggctgcgtgg
   104641 cggcctgtgg tctggcgggt ggttttactc tgccaaccac ggtcatgcca tttattctgc
   104701 gtaatgtccg tttgcaaggg gtggattcag taatgacgcc accagaacgc cgcgcacaag
   104761 cctggcagcg actggtcgcc gatttaccgg aatcattcta tacccaggcg gcaaaagaga
   104821 tatctctgtc agaggcaccg aactttgccg aggccatcat taataaccag atccagggtc
   104881 gcacgctggt gaaggttaac taaccattta gcaaggaata ataagagagg gaactcattt
   104941 tgaaatcatt attcaatcgg ttaacgggaa aagcggttag ccggacagct ttcgtcgaac
   105001 accttggtca tgaagttgta caacatcatc caaactggaa agtcatgatt tcgactgacc
   105061 ataaattgat gcgcattgat actccactaa acagctatta ttgatacgcc tccgtcgcct
   105121 gttaggttta tggtgctttg cctgggcgac gctacgctta accccttact tatttctggt
   105181 accatgaggt gaataatctg attttgtttg actacaaatt aatcactcga acctatttaa
   105241 tgctgagcat tgtcaatcgg ttaattttgc gtgctttagc attcacatct atccaggcga
   105301 tgcagtgaaa attgggtaat ccccagcaac cgctgcgtaa tgtcgtctat cttgtcgcga
   105361 tcctggcatc cctacattat ttgtggtctg tgacgattat ctcattgcag cccctcatct
   105421 tcgcagggct ggctttacag cttttcacct tacgttataa gaagttccgt cgatgatggc
   105481 gctaatttcg tgaattgtgc ggcttgttgc aaattacacg gtgttgaagg ttatttacat
   105541 gttagttgtt gattatcttc cctgataaga ccagtattta gctgccaatt gctacgaaat
   105601 cgttataatg tgcgacctcg tcctccctga cgcagttttt gcgctgcgga aaaggtgaca
   105661 ttggcgcaac gaaggtatat tttgtttttt gccggaggat agcagcagat cgctgcacaa
   105721 tgtccgtcaa gtctaacatt gacactctgg ggcaaaatag accggcgtcc cgacctgctg
   105781 gaatttatcg ctatgcatac agctgtcggg gcatacgctt tacagacggc ggtgaaacgc
   105841 ctgtcacaat cacactaaac aaagagtacg gaacccactc atggatattc gtaagattaa
   105901 aaaactgatc gagctggttg aagaatcagg catctccgaa ctggaaattt ctgaaggcga
   105961 agagtcagta cgcattagcc gtgcagctcc tgccgcaagt ttccctgtga tgcaacaagc
   106021 ttacgctgca ccaatgatgc agcagccagc tcaatctaac gcagccgctc cggcgaccgt
   106081 tccttccatg gaagcgccag cagcagcgga aatcagtggt cacatcgtac gttccccgat
   106141 ggttggtact ttctaccgca ccccaagccc ggatgcaaaa gcgttcatcg aagtgggtca
   106201 gaaagtcaac gtgggcgata ccctgtgcat cgttgaagcc atgaaaatga tgaaccagat
   106261 cgaagcggac aaatccggta ccgtgaaagc aattctggtc gaaagtggac aaccggtaga
   106321 atttgacgag ccgctggtcg tcatcgagta acgaggcgaa catgctggat aaaattgtta
   106381 ttgccaaccg cggcgagatt gcattgcgta ttcttcgtgc ctgtaaagaa ctgggcatca
   106441 agactgtcgc tgtgcactcc agcgcggatc gcgatctaaa acacgtatta ctggcagatg
   106501 aaacggtctg tattggccct gctccgtcag taaaaagtta tctgaacatc ccggcaatca
   106561 tcagcgccgc tgaaatcacc ggcgcagtag caatccatcc gggttacggc ttcctctccg
   106621 agaacgccaa ctttgccgag caggttgaac gctccggctt tatcttcatt ggcccgaaag
   106681 cagaaaccat tcgcctgatg ggcgacaaag tatccgcaat cgcggcgatg aaaaaagcgg
   106741 gcgtcccttg cgtaccgggt tctgacggcc cgctgggcga cgatatggat aaaaaccgtg
   106801 ccattgctaa acgcattggt tatccggtga ttatcaaagc ctccggcggc ggcggtggtc
   106861 gcggtatgcg cgtagtgcgc ggcgacgctg aactggcaca atccatctcc atgacccgtg
   106921 cggaagcgaa agctgctttc agcaacgata tggtttacat ggagaaatac ctggaaaatc
   106981 ctcgccacgt cgagattcag gtactggctg acggtcaggg caactctatc tatctggcgg
   107041 aacgtgactg ctccatgcag cgccgccacc agaaagtggt cgaagaagca ccagcaccgg
   107101 gcattacccc ggaactgcgt cgctacatcg gcgaacgttg cgctaaagcg tgtgttgata
   107161 tcggctatcg cggtgcaggt actttcgagt tcctgttcga aaacggcgag ttctatttca
   107221 tcgaaatgaa cacccgtatt caggtagaac acccggttac agaaatgatc accggcgttg
   107281 acctgatcaa agaacagctg cgtatcgctg ccggtcaacc gctgtcgatc aagcaagaag
   107341 aagttcacgt tcgcggccat gcggtggaat gtcgtatcaa cgccgaagat ccgaacacct
   107401 tcctgccaag tccgggcaaa atcacccgtt tccacgcacc tggcggtttt ggcgtacgtt
   107461 gggagtctca tatctacgcg ggctacaccg taccgccgta ctatgactca atgatcggta
   107521 agctgatttg ctacggtgaa aaccgtgacg tggcgattgc ccgcatgaag aatgcgctgc
   107581 aggagctgat catcgacggt atcaaaacca acgttgatct gcagatccgc atcatgaatg
   107641 acgagaactt ccagcatggt ggcactaaca tccactatct ggagaaaaaa ctcggtcttc
   107701 aggaaaaata agactgcaaa agcgtcaaaa ggccggattt tccggccttt tttattactg
   107761 gggatcgaca acacccataa ggtacaatcc tcgctttctt cacccatcag ggacaaaaaa
   107821 tggacactcg ttttgttcag gcccataaag aggcgcgctg ggcgctgggg ctgacccttt
   107881 tgtatctggc agtttggtta gtagccgctt acttacctgg cgttgccccc ggttttaccg
   107941 gctttccgcg ctggtttgag atggcctgca tcctgacgcc gctgctgttt attggactgt
   108001 gctgggcgat ggtgaaattt atctatcgcg atatcccgct ggaggatgac gatgcagctt
   108061 gaagtaattc taccgctggt cgcctatctg gtggtggtgt tcggtatctc ggtttatgcg
   108121 atgcgtaaac ggagcaccgg caccttcctt aatgagtatt tcctcggcag ccgctctatg
   108181 ggcggtattg tgctggcgat gacgctcacc gcgacttata tcagtgccag ttcgtttatc
   108241 ggcgggcctg gagctgctta caaatacggc cttggctggg tattgctggc gatgattcag
   108301 cttccggcag tctggctttc actcggtatt ctcggcaaga agtttgcgat tcttgcgcgc
   108361 cgctacaatg cagtgacgct gaacgatatg ctgtttgccc gctaccagag tcgtcttctg
   108421 gtgtggctgg cgagtttgag tttgctggtt gcgttcgttg gtgcgatgac cgtgcagttt
   108481 atcggcggtg cgcgcctgct ggaaaccgcg gcgggtattc cttatgaaac cgggctgctg
   108541 atttttggta tcagcattgc gttatatacc gcctttggtg gctttcgcgc cagcgtgctg
   108601 aacgacacca tgcaagggct tgtgatgctg attggcaccg ttgtgctgct tattggcgtg
   108661 gtacatgccg ctggcggctt aagcaacgca gtacagacct tgcaaaccat cgatccgcaa
   108721 ctggttacgc cacaaggcgc tgacgatatt ctgtcgcctg cctttatgac gtcgttctgg
   108781 gtactggtgt gctttggcgt tataggcctg ccgcatactg cggtgcgctg tatctcttat
   108841 aaagacagca aagcagtaca ccgggggatc atcatcggta cgattgtagt cgcaatcctg
   108901 atgttcggta tgcacctagc cggagcgtta ggtcgtgcag tgatccccga tctcaccgta
   108961 ccggatctgg tgatcccgac gttaatggta aaagtgctgc caccgtttgc tgccgggatc
   109021 tttctggctg cacctatggc tgcgatcatg tcgacaatta acgcccaatt gctacaaagt
   109081 tccgctacga tcattaaaga tctctatctg aatatccgtc cggatcaaat gcaaaacgag
   109141 acgcgtctga agcggatgtc ggcggtaatt acgttagttc tcggcgcgtt gctgctgctt
   109201 gccgcctgga agccgccaga aatgatcatc tggctgaatt tgctggcctt cggtgggctg
   109261 gaagccgttt tcctgtggcc gctggtgctg ggtctttact gggaacgcgc caacgccaaa
   109321 ggcgcgctaa gtgcgatgat cgttggcggc gtgctgtatg ccgtactcgc gacgctgaat
   109381 attcagtacc tgggcttcca ccctatcgtg ccctcgttac tactaagttt gctggctttc
   109441 ctggtcggaa accgtttcgg tacatccgtc ccgcaagcta ccgttttgac tactgataaa
   109501 taaagagttt tgccatgcct tggatccaac tgaaactgaa caccaccggc gcgaacgcgg
   109561 aagatcttag cgatgcgctg atggaagcgg gtgccgtttc tatcactttt caggataccc
   109621 acgatacgcc agtatttgag ccgctgccgg gcgaaacgcg cctgtggggc gacaccgatg
   109681 tgattggtct gttcgacgct gaaaccgata tgaacgacgt ggtggcgatt ctggaaaacc
   109741 atccgctgct cggcgcaggc ttcgcgcata aaatcgaaca actagaagat aaagactggg
   109801 agcgcgaatg gatggataat ttccacccga tgcgctttgg cgaacgactg tggatctgcc
   109861 ctagctggcg tgatgtgccg gacgaaaacg ccgtcaacgt gatgttagat ccagggctgg
   109921 cgtttggtac gggtacccat ccaaccacct ctctgtgcct gcaatggctc gacagcctcg
   109981 atttaaccgg taaaacagtc atcgactttg gctgtggttc cggcattctg gcgatcgcgg
   110041 cgctgaaact gggtgcagca aaagccattg gtattgatat cgatccgcag gcgattcagg
   110101 ccagccgcga taacgccgaa cgtaatggcg tttctgaccg tctggaactc tacttaccga
   110161 aagatcagcc agaagaaatg aaagccgacg tggtggtcgc taacatcctt gcaggcccat
   110221 tacgtgaact ggcaccgtta attagcgtcc tgccagtttc aggcggctta ctgggccttt
   110281 ctggtattct ggcaagccag gcggagagcg tttgcgaagc ctatgccgac agcttcgctc
   110341 tggacccggt cgtggaaaaa gaagagtggt gccgtattac cggtcgtaag aattaacctt
   110401 cgcatcgcca tagggtgacg cggggcgaag tgcgagcaag ctcacaaaag gcacgtaaat
   110461 ttgccgatta tttacgcaaa tttgcgtgcc aaaattttca ttcataaaga aaaattgaga
   110521 acttactcaa atttctttga gtgtaaattt tagtcactat tttctaatat gatgattttt
   110581 atgagtaatt atcgcaccac gctcatttta aatgcaattc tttgatccat ctcagaggat
   110641 tggtcaaagt ttggcctttc atctcgtgca aaaaatgcgt aatatacgcc gccttgcagt
   110701 cacagtatgg tcatttctta actcatgcgc atcggacaat atcagctcag aaatcgcctg
   110761 atcgcagcgc ccatggctgg cattacagac agaccttttc ggacgttgtg ctacgagatg
   110821 ggagccggat tgacagtatc cgagatgatg tcttctaacc cacaggtttg ggaaagcgac
   110881 aaatctcgtt tacggatggt gcacattgat gaacccggta ttcgcaccgt gcaaattgct
   110941 ggtagcgatc cgaaagaaat ggcagatgca gcacgtatta acgtggaaag cggtgcccag
   111001 attattgata tcaatatggg ttgcccggct aaaaaagtga atcgcaagct cgcaggttca
   111061 gccctcttgc agtacccgga tgtcgttaaa tcgatcctta ccgaggtcgt caatgcagtg
   111121 gacgttcctg ttaccctgaa gattcgcacc ggctgggcac cggaacaccg taactgcgaa
   111181 gagattgccc aactggctga agactgtggc attcaggctc tgaccattca tggccgtaca
   111241 cgcgcctgtt tgttcaatgg agaagctgag tacgacagta ttcgggcagt taagcagaaa
   111301 gtttccattc cggttatcgc gaatggcgac attactgacc cgcttaaagc cagagctgtg
   111361 ctcgactata caggggcgga tgccctgatg ataggccgcg cagctcaggg aagaccctgg
   111421 atctttcggg aaatccagca ttatctggac actggggagt tgctgccccc gctgcctttg
   111481 gcagaggtta agcgcttgct ttgcgcgcac gttcgggaac tgcatgactt ttatggtccg
   111541 gcaaaagggt accgaattgc acgtaaacac gtttcctggt atctccagga acacgctcca
   111601 aatgaccagt ttcggcgcac attcaacgcc attgaggatg ccagcgaaca gctggaggcg
   111661 ttggaggcat acttcgaaaa ttttgcgtaa acagaaataa agagctgaca gaactatgtt
   111721 cgaacaacgc gtaaattctg acgtactgac cgtttctacc gttaactctc aggatcaggt
   111781 aacccaaaaa cccctgcgtg actcggttaa acaggcactg aagaactatt ttgctcaact
   111841 gaatggtcag gatgtgaatg acctctatga gctggtactg gctgaagtag aacagcccct
   111901 gttggacatg gtgatgcaat acacccgtgg taaccagacc cgtgctgcgc tgatgatggg
   111961 catcaaccgt ggtacgctgc gtaaaaaatt gaaaaaatac ggcatgaact aattcaggtt
   112021 agctaattgc ttgattaaaa ggcgctactc ggcatgggga agcgcctttt ttataggtgt
   112081 cacaaaggga gtgaccatga gaacaggatg tgaaccgacc cggtttggta atgaagctaa
   112141 gaccattatt cacggtgatg cccttgccga acttaaaaag ctacctactg aaagcgtcga
   112201 tctgatcttt gccgacccac cgtataacat cggtaaaaat tttgatggtc tgatcgaagc
   112261 ctggaaagaa gatctgttta tcgactggct gttagaagtg attgcagagt gccaccgcgt
   112321 tctgaaaaag cagggcagca tgtacatcat gaacagtacg gaaaacatgc cctttatcga
   112381 tctccagtgc cgcaagcttt ttaccatcaa aagtcgcatc gtctggtcat atgacagttc
   112441 tggagtgcag gcgaaaaaac actacggctc catgtacgaa cccatcctga tgatggtgaa
   112501 agacgcaaag aactacacat tcaacggtga tgctattctg gtagaagcca aaaccggatc
   112561 gcagcgcgcg ttgatcgatt atcgcaaaaa tcctccacag ccttacaatc atcaaaaagt
   112621 accgggtaac gtctgggatt ttccgcgcgt gcgttatttg atggatgaat atgaaaacca
   112681 cccgacgcaa aaaccggaag ccttactgaa acgcattatt ctcgcctctt ccaacccagg
   112741 cgatatcgtt ctcgacccgt ttgctggtag ctttactacc ggtgccgtag ccatcgccag
   112801 cggacgaaaa ttcattggta ttgagatcaa cagcgagtac atcaaaatgg ggcttcgacg
   112861 gctggatgtc gcgtcgcatt actctgcgga agaactggcg aaagtgaaaa aaagaaagac
   112921 cggcaacctg tcaaaacgaa gccggttaag cgaagttgac cccgatctca ttacaaagta
   112981 aaggatgtgt aagcctggtt tgcagattat tcatttcgtg tatatttcct gccagacttg
   113041 gttaaacatg cacaggctca ggtaatgatt cgcaagtatt ggtggctcgt cgttttcgct
   113101 gtcttcgttt ttctgtttga tactttactg atgcagtgga ttgaactgct ggcaacagaa
   113161 acagacaaat gccgcaatat gaactcagtt aatccactaa aactggttaa ctgtgacgaa
   113221 ctgaattttc aggacagaat gtgaatttac atgacactta attcattcgt ttgatgaatt
   113281 aatttcgtta tgttttcatc tggcatgaac attcttaata cgttatcgac cagagcgggg
   113341 gcttgtttat aaagatcata acccgccata ttcattaacc agttttgaac aattccgctg
   113401 aaggcaccat caataataat catcacaaca tctaaatcga ggttatttgc tacacaacct
   113461 tgttgctgac acgcctgcaa tacttcgcgg agagtctgcg gattaaagcc catcttttcg
   113521 cgtatcactc cctcggccag catctcatca ttaaattcac atttgtgata taagattttc
   113581 agcaacgcct gctggcgggg aattttggca atatattgca agccgacaat caatttttca
   113641 cgcaattgtt gaaacgggtc atgctctaat ccagccgtca agtgttcctg gattaactcc
   113701 cgcaatgaag gctgttgcaa ccacatctca ttaaacagtt gggtcttgtt ctcgaagtgc
   113761 cagtagatag cgccacgcgt aacgttagcg gcatcggcaa tgtcgttgag cgtcgtcttg
   113821 cttacgccat gttgcgcaaa ctgggcgatg gcagtttcaa tcagttcttg ccgggtcttc
   113881 agagcttcgg ctttggttct ttttgccatg attaattatt caggaaataa atatcttcga
   113941 cacagagtga gaaaatagcg aaggt
//
LOCUS       DAZFDC010000002       109961 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFDC010000002 DAZFDC010000000
VERSION     DAZFDC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 109961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.002"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHJ48_000114"
     CDS             complement(<1..209)
                     /locus_tag="ACHJ48_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4705753.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHJ48_000115"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHJ48_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO4705754.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHJ48_000116"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHJ48_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO4705755.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHJ48_000117"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHJ48_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO4705756.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHJ48_000118"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHJ48_000118"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO4705757.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSGQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHJ48_000119"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHJ48_000119"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO4705758.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLDLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHJ48_000120"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHJ48_000120"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO4705759.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDEMEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHJ48_000121"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHJ48_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4705760.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHJ48_000122"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHJ48_000122"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4705761.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHJ48_000123"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHJ48_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO4705762.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHJ48_000124"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHJ48_000124"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4705763.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHJ48_000125"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHJ48_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO4705764.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHJ48_000126"
     CDS             complement(7709..9487)
                     /gene="kefB"
                     /locus_tag="ACHJ48_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO4705765.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERR"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHJ48_000127"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHJ48_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO4705766.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHJ48_000128"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHJ48_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4705767.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHJ48_000129"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHJ48_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4705768.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHJ48_000130"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHJ48_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO4705769.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHJ48_000131"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHJ48_000131"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO4705770.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAIELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHJ48_000132"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHJ48_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO4705771.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHJ48_000133"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHJ48_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO4705772.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHJ48_000134"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHJ48_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO4705773.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHJ48_000135"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHJ48_000135"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO4705774.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPSDIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHJ48_000136"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHJ48_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO4705775.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKVVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHJ48_000137"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHJ48_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO4705776.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHJ48_000138"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHJ48_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO4705777.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHJ48_000139"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHJ48_000139"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO4705778.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHJ48_000140"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHJ48_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO4705779.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHJ48_000141"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHJ48_000141"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO4705780.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHJ48_000142"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHJ48_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO4705781.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHJ48_000143"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHJ48_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO4705782.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHJ48_000144"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHJ48_000144"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO4705783.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28336..28503
                     /gene="yhfL"
                     /locus_tag="ACHJ48_000145"
     CDS             28336..28503
                     /gene="yhfL"
                     /locus_tag="ACHJ48_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO4705784.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28799..30136
                     /gene="frlA"
                     /locus_tag="ACHJ48_000146"
     CDS             28799..30136
                     /gene="frlA"
                     /locus_tag="ACHJ48_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO4705785.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPATTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFSLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTPLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30157..31179
                     /gene="frlB"
                     /locus_tag="ACHJ48_000147"
     CDS             30157..31179
                     /gene="frlB"
                     /locus_tag="ACHJ48_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO4705786.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31230..32056
                     /gene="frlC"
                     /locus_tag="ACHJ48_000148"
                     /pseudo
     CDS             31230..32056
                     /gene="frlC"
                     /locus_tag="ACHJ48_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32056..32841
                     /gene="frlD"
                     /locus_tag="ACHJ48_000149"
                     /pseudo
     CDS             32056..32841
                     /gene="frlD"
                     /locus_tag="ACHJ48_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            32941..33672
                     /gene="frlR"
                     /locus_tag="ACHJ48_000150"
                     /gene_synonym="yhfR"
     CDS             32941..33672
                     /gene="frlR"
                     /locus_tag="ACHJ48_000150"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO4705787.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33803..34807)
                     /gene="trpS"
                     /locus_tag="ACHJ48_000151"
     CDS             complement(33803..34807)
                     /gene="trpS"
                     /locus_tag="ACHJ48_000151"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO4705788.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(34800..35558)
                     /gene="gph"
                     /locus_tag="ACHJ48_000152"
     CDS             complement(34800..35558)
                     /gene="gph"
                     /locus_tag="ACHJ48_000152"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO4705789.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(35551..36228)
                     /gene="rpe"
                     /locus_tag="ACHJ48_000153"
     CDS             complement(35551..36228)
                     /gene="rpe"
                     /locus_tag="ACHJ48_000153"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO4705790.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(36246..37082)
                     /gene="dam"
                     /locus_tag="ACHJ48_000154"
     CDS             complement(36246..37082)
                     /gene="dam"
                     /locus_tag="ACHJ48_000154"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO4705791.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(37189..38475)
                     /gene="damX"
                     /locus_tag="ACHJ48_000155"
     CDS             complement(37189..38475)
                     /gene="damX"
                     /locus_tag="ACHJ48_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO4705792.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(38567..39655)
                     /gene="aroB"
                     /locus_tag="ACHJ48_000156"
     CDS             complement(38567..39655)
                     /gene="aroB"
                     /locus_tag="ACHJ48_000156"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO4705793.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGTFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(39712..40233)
                     /gene="aroK"
                     /locus_tag="ACHJ48_000157"
     CDS             complement(39712..40233)
                     /gene="aroK"
                     /locus_tag="ACHJ48_000157"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO4705794.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(40634..41872)
                     /gene="hofQ"
                     /locus_tag="ACHJ48_000158"
     CDS             complement(40634..41872)
                     /gene="hofQ"
                     /locus_tag="ACHJ48_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO4705795.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(41784..42188)
                     /gene="hofP"
                     /locus_tag="ACHJ48_000159"
     CDS             complement(41784..42188)
                     /gene="hofP"
                     /locus_tag="ACHJ48_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO4705796.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTLDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(42178..42618)
                     /gene="hofO"
                     /locus_tag="ACHJ48_000160"
     CDS             complement(42178..42618)
                     /gene="hofO"
                     /locus_tag="ACHJ48_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO4705797.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(42602..43141)
                     /gene="hofN"
                     /locus_tag="ACHJ48_000161"
     CDS             complement(42602..43141)
                     /gene="hofN"
                     /locus_tag="ACHJ48_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO4705798.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(43125..43919)
                     /gene="hofM"
                     /locus_tag="ACHJ48_000162"
     CDS             complement(43125..43919)
                     /gene="hofM"
                     /locus_tag="ACHJ48_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO4705799.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGWRLGRRTDEPAN"
     gene            44039..46591
                     /gene="mrcA"
                     /locus_tag="ACHJ48_000163"
     CDS             44039..46591
                     /gene="mrcA"
                     /locus_tag="ACHJ48_000163"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO4705800.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(46756..47316)
                     /gene="nudE"
                     /locus_tag="ACHJ48_000164"
     CDS             complement(46756..47316)
                     /gene="nudE"
                     /locus_tag="ACHJ48_000164"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO4705801.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            47636..49771
                     /gene="igaA"
                     /locus_tag="ACHJ48_000165"
     CDS             47636..49771
                     /gene="igaA"
                     /locus_tag="ACHJ48_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO4705802.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHLVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            49836..50504
                     /gene="yrfG"
                     /locus_tag="ACHJ48_000166"
     CDS             49836..50504
                     /gene="yrfG"
                     /locus_tag="ACHJ48_000166"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO4705803.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            50515..50916
                     /gene="hslR"
                     /locus_tag="ACHJ48_000167"
     CDS             50515..50916
                     /gene="hslR"
                     /locus_tag="ACHJ48_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO4705804.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            50941..51819
                     /gene="hslO"
                     /locus_tag="ACHJ48_000168"
     CDS             50941..51819
                     /gene="hslO"
                     /locus_tag="ACHJ48_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO4705805.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(51882..53606)
                     /gene="yhgE"
                     /locus_tag="ACHJ48_000169"
     CDS             complement(51882..53606)
                     /gene="yhgE"
                     /locus_tag="ACHJ48_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO4705806.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFIAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            53985..55607
                     /gene="pckA"
                     /locus_tag="ACHJ48_000170"
     CDS             53985..55607
                     /gene="pckA"
                     /locus_tag="ACHJ48_000170"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO4705807.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVSEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(55683..57035)
                     /gene="envZ"
                     /locus_tag="ACHJ48_000171"
     CDS             complement(55683..57035)
                     /gene="envZ"
                     /locus_tag="ACHJ48_000171"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO4705808.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQSTTKEG
                     "
     gene            complement(57032..57751)
                     /gene="ompR"
                     /locus_tag="ACHJ48_000172"
     CDS             complement(57032..57751)
                     /gene="ompR"
                     /locus_tag="ACHJ48_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO4705809.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            57979..58455
                     /gene="greB"
                     /locus_tag="ACHJ48_000173"
     CDS             57979..58455
                     /gene="greB"
                     /locus_tag="ACHJ48_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO4705810.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            58552..60873
                     /gene="yhgF"
                     /locus_tag="ACHJ48_000174"
     CDS             58552..60873
                     /gene="yhgF"
                     /locus_tag="ACHJ48_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO4705811.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLGYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAENEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            61311..61538
                     /gene="feoA"
                     /locus_tag="ACHJ48_000175"
     CDS             61311..61538
                     /gene="feoA"
                     /locus_tag="ACHJ48_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO4705812.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            61555..63876
                     /gene="feoB"
                     /locus_tag="ACHJ48_000176"
     CDS             61555..63876
                     /gene="feoB"
                     /locus_tag="ACHJ48_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO4705813.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            63876..64112
                     /gene="feoC"
                     /locus_tag="ACHJ48_000177"
     CDS             63876..64112
                     /gene="feoC"
                     /locus_tag="ACHJ48_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO4705814.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            64315..65193
                     /gene="rpnA"
                     /locus_tag="ACHJ48_000178"
     CDS             64315..65193
                     /gene="rpnA"
                     /locus_tag="ACHJ48_000178"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO4705815.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMTLLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(65220..65990)
                     /gene="bioH"
                     /locus_tag="ACHJ48_000179"
     CDS             complement(65220..65990)
                     /gene="bioH"
                     /locus_tag="ACHJ48_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO4705816.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLAEMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRLPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            66028..66711
                     /gene="gntX"
                     /locus_tag="ACHJ48_000180"
     CDS             66028..66711
                     /gene="gntX"
                     /locus_tag="ACHJ48_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO4705817.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAITRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            66770..67345
                     /gene="nfuA"
                     /locus_tag="ACHJ48_000181"
     CDS             66770..67345
                     /gene="nfuA"
                     /locus_tag="ACHJ48_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO4705818.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            67706..69022
                     /gene="gntT"
                     /locus_tag="ACHJ48_000182"
     CDS             67706..69022
                     /gene="gntT"
                     /locus_tag="ACHJ48_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4705819.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(69067..71151)
                     /gene="malQ"
                     /locus_tag="ACHJ48_000183"
     CDS             complement(69067..71151)
                     /gene="malQ"
                     /locus_tag="ACHJ48_000183"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO4705820.1"
                     /translation="MESKRLDNAALAAGISPHYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRGHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWQAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(71161..73554)
                     /gene="malP"
                     /locus_tag="ACHJ48_000184"
     CDS             complement(71161..73554)
                     /gene="malP"
                     /locus_tag="ACHJ48_000184"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO4705821.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            74166..76871
                     /gene="malT"
                     /locus_tag="ACHJ48_000185"
     CDS             74166..76871
                     /gene="malT"
                     /locus_tag="ACHJ48_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO4705822.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            76938..77216
                     /locus_tag="ACHJ48_000186"
     CDS             76938..77216
                     /locus_tag="ACHJ48_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO4705823.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            77207..77692
                     /locus_tag="ACHJ48_000187"
     CDS             77207..77692
                     /locus_tag="ACHJ48_000187"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4705824.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
                     E"
     gene            complement(77742..78770)
                     /gene="rtcA"
                     /locus_tag="ACHJ48_000188"
                     /pseudo
     CDS             complement(77742..78770)
                     /gene="rtcA"
                     /locus_tag="ACHJ48_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            complement(78774..80000)
                     /gene="rtcB"
                     /locus_tag="ACHJ48_000189"
     CDS             complement(78774..80000)
                     /gene="rtcB"
                     /locus_tag="ACHJ48_000189"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO4705825.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATVHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            80188..81786
                     /gene="rtcR"
                     /locus_tag="ACHJ48_000190"
     CDS             80188..81786
                     /gene="rtcR"
                     /locus_tag="ACHJ48_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO4705826.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSLYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVADGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(81768..82526)
                     /locus_tag="ACHJ48_000191"
     CDS             complement(81768..82526)
                     /locus_tag="ACHJ48_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO4705827.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTPPPVSVMQVLTDHHIQLELC"
     gene            complement(82543..83373)
                     /gene="glpG"
                     /locus_tag="ACHJ48_000192"
     CDS             complement(82543..83373)
                     /gene="glpG"
                     /locus_tag="ACHJ48_000192"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO4705828.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(83418..83744)
                     /gene="glpE"
                     /locus_tag="ACHJ48_000193"
     CDS             complement(83418..83744)
                     /gene="glpE"
                     /locus_tag="ACHJ48_000193"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO4705829.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRNNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            83934..85439
                     /gene="glpD"
                     /locus_tag="ACHJ48_000194"
     CDS             83934..85439
                     /gene="glpD"
                     /locus_tag="ACHJ48_000194"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO4705830.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(85654..86256)
                     /locus_tag="ACHJ48_000195"
     CDS             complement(85654..86256)
                     /locus_tag="ACHJ48_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4705831.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPTVVALAACVITLSFLGEIL
                     FSIPLMHRRISLLQLQLWLSAEYALFFCAMVGVGWQFARRTPPELKSRLHCWLVFAPV
                     YFWLLLWNIIFYVAPAQIALLENMRSFFLTVVWLPLNFSPFWSQQWIDFVGPISAQLG
                     FALGYYCQWRSKNRSQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(86256..87248)
                     /locus_tag="ACHJ48_000196"
     CDS             complement(86256..87248)
                     /locus_tag="ACHJ48_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4705832.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(87274..88782)
                     /locus_tag="ACHJ48_000197"
     CDS             complement(87274..88782)
                     /locus_tag="ACHJ48_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4705833.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLTGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIITLHLADYQFRQMGAGWLANLMLPWLGVFLVSLVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIRAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKKVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIRLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYLTME"
     gene            complement(88907..91354)
                     /gene="glgP"
                     /locus_tag="ACHJ48_000198"
     CDS             complement(88907..91354)
                     /gene="glgP"
                     /locus_tag="ACHJ48_000198"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO4705834.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADLDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(91373..92806)
                     /gene="glgA"
                     /locus_tag="ACHJ48_000199"
     CDS             complement(91373..92806)
                     /gene="glgA"
                     /locus_tag="ACHJ48_000199"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO4705835.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(92806..94101)
                     /gene="glgC"
                     /locus_tag="ACHJ48_000200"
     CDS             complement(92806..94101)
                     /gene="glgC"
                     /locus_tag="ACHJ48_000200"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO4705836.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(94119..96092)
                     /gene="glgX"
                     /locus_tag="ACHJ48_000201"
     CDS             complement(94119..96092)
                     /gene="glgX"
                     /locus_tag="ACHJ48_000201"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO4705837.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGVGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTVALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(96089..98275)
                     /gene="glgB"
                     /locus_tag="ACHJ48_000202"
     CDS             complement(96089..98275)
                     /gene="glgB"
                     /locus_tag="ACHJ48_000202"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO4705838.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPLNEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(98548..99651)
                     /gene="asd"
                     /locus_tag="ACHJ48_000203"
     CDS             complement(98548..99651)
                     /gene="asd"
                     /locus_tag="ACHJ48_000203"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4705839.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            99843..100436
                     /gene="yhgN"
                     /locus_tag="ACHJ48_000204"
     CDS             99843..100436
                     /gene="yhgN"
                     /locus_tag="ACHJ48_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO4705840.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(100482..101777)
                     /locus_tag="ACHJ48_000205"
     CDS             complement(100482..101777)
                     /locus_tag="ACHJ48_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO4705841.1"
                     /translation="MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(101684..103808)
                     /locus_tag="ACHJ48_000206"
                     /pseudo
     CDS             complement(101684..103808)
                     /locus_tag="ACHJ48_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
     gene            complement(103948..105288)
                     /gene="gntU"
                     /locus_tag="ACHJ48_000207"
     CDS             complement(103948..105288)
                     /gene="gntU"
                     /locus_tag="ACHJ48_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO4705842.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(105292..105819)
                     /gene="gntK"
                     /locus_tag="ACHJ48_000208"
     CDS             complement(105292..105819)
                     /gene="gntK"
                     /locus_tag="ACHJ48_000208"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO4705843.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(105958..106953)
                     /gene="gntR"
                     /locus_tag="ACHJ48_000209"
     CDS             complement(105958..106953)
                     /gene="gntR"
                     /locus_tag="ACHJ48_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO4705844.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(107177..107872)
                     /gene="yhhW"
                     /locus_tag="ACHJ48_000210"
     CDS             complement(107177..107872)
                     /gene="yhhW"
                     /locus_tag="ACHJ48_000210"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO4705845.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASISDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(107995..109032)
                     /gene="yhhX"
                     /locus_tag="ACHJ48_000211"
     CDS             complement(107995..109032)
                     /gene="yhhX"
                     /locus_tag="ACHJ48_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4705846.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRAMEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            109366..109854
                     /gene="yhhY"
                     /locus_tag="ACHJ48_000212"
     CDS             109366..109854
                     /gene="yhhY"
                     /locus_tag="ACHJ48_000212"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4705847.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
BASE COUNT        26562 a        29772 c        27608 g        26019 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcctgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggccggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagatcca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccattt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctact ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccgc aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct agcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc ataccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatctacct gcgtggcatc accgtaataa actttgtagc
     8161 catatttgcg catcaggtta acggcgctga tatcccgctc cagcacagta atgcgcattt
     8221 tatttgccat caacaaacga ccaatcacct gcccaaaacg tccaaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat cccgacagag ataaagaaca aaccgagcag caagccttta aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgcct gcaataaacg
     8761 taccgagcgc catcgacagc cccaacgcat ccataaacaa tgcggaaccc aataccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcaataa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcaagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcaggacat cgctcatcgg
     9661 gtaacgattc aacgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacact
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 tggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gctgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgtcgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaat ctggtccccg gttcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagtggtg agataggcct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcgcct attcaacatc tggcacgttt agcgccgcag gaactggagc aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatc gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgaccttgat atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcgact ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggc catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccggttgat
    12601 gcggcggtta ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagcc gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt acctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg attcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtgcccgg
    14161 cggcaaaatg ggtctggcaa ttgaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggctgtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctcaatc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc ttgaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atattaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccttttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccga cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctattggc acttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcgct
    16741 gaaaagttat ttgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcttga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cgcaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga ccttgatgct gattaccacc cttgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggatccg caaccaacgc cactggcctg gcagcgaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaacca gcgttcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag tcagtttatt gttgaacata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat gcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cctgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacaact
    18421 cacgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgatggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacgc aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttatg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacttcaaag cacgctggta acgagtcagg ttccactacc
    19321 agcgaatgat agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ctttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca acgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgact cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctgaaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgaaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagcgaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaat gatgctggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccggcac atatggcgtg gtcaattctc gccctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctcatgcc ggacggcact atcgtcgtcc ggccttttcc tctctaaccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aattaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcagaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgagagcatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgttgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagcaaag agatctacaa acgccttatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgtc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactatt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggttacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcgg taggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgtctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcggcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtactg ccgacaaact gacgcgtacc
    25141 gcaccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaatacg ccgtctgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccagta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtcgcgatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg actgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagc gacggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc gcgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaataacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cctgattttt acgctcggta atttgctcga
    26041 tccatccgta cgccctctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgagctgt tcaccggaca caccatgttc ctcacctttg gggtgaaagc
    26161 gggcaccatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggt ggtggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgtctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgcaat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactccatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc tgccagcttc attatgccgt cgattattga
    27061 ccgctcaccg ctgatggtgg cggtttcctc tggcggtact tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gctgcatctg ggccaggtag cgaaatacgc
    27181 cgggcagtta cgcggtcggg taaaacaaca gttcgccacg atgggtgaac gccgccgctt
    27241 ctgggaaaaa ctgttcgtta acgaccgtct ggcgcagtcg ctggcgaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acactgaaag gtctgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgcggatc gtgttttcgt cggcaaacgc gcgggatacc actgcgtacc
    27541 gcaggaggag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggc ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctcagc
    27721 ctattcgggt attccgctca cgcatcgcga ttatgcccag agcgtgcgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggctga atcaggctgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tagcaattgt cgaaaacgga acagcagtca cgcagcgcgt
    27961 gattgacggt acgctcacac aactgggcga actggcgcag caaatgaata gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaatcag ggccagagaa ttctggcctt tttcgtttac aactctcttt atatattcag
    28141 cagataaaaa tataactcat taacaaaaat catttgtttt tgccccgtta tattcaccgt
    28201 tacagcgact tacaaaatta agtcattcac tcactgacgc cgattaaata ttcctgtatc
    28261 gttcttaatg agcagtctac ttatattcat ataatcaatg aatattaatc aatatataat
    28321 acatagggaa tgtaaatgaa caaatttatt aaagttgcac tggtaggtgc agtactggct
    28381 acgttaactg catgtactgg tcatattgaa aaccgtgata agaactgctc ttacgactac
    28441 ctgctgcacc cagcaatttc tatttctaaa atcattggcg gttgcggtcc tactgcacag
    28501 taagtctctg acagaaatca gctaacgctg atttaattgc ctgacgccct cgggattacc
    28561 cgagggcgta tttgtttcta tatccttctt gctccccacc cgtaaaaata ttttcacttt
    28621 aatttcaatt ggataaccac attcatcagc cacagcgcga tcctgtgcga aattatgtga
    28681 tcttcctcca cattacataa catcatatgt tgttatattc atcatgcatt gtcatgtcac
    28741 cttttaaatg actgcaaact ctcccctaca acgtgttgga caagaaaaag gtatcgctat
    28801 gggaagccag gaactccaac gcaagctcgg attttgggcc gttcttgcaa tcgccgtcgg
    28861 gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa gtggcaaaag cggcgggcac
    28921 gccgtggctg acggtgctcg catttgtcat cggcgggtta attgtgatcc cgcaaatgtg
    28981 cgtatatgcg gaactatcca ccgcttatcc ggaaaatggc gcagattatg tttatctgaa
    29041 aaatgccgga agccgaccgc tggctttcct ctccggctgg gccagcttct gggccaacga
    29101 tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc aatcttggct ttttaacgcc
    29161 tatcgatccg ttgctcggta aatttatcgc cgccggatta attatcgcct ttatgctgct
    29221 gcacctgcgc tccgttgaag gcggcgcagc gtttcagacg ctaattacca tcgccaaaat
    29281 tatcccgttc actatcgtca ttggtcttgg gatcttctgg tttaaagcgg agaattttgc
    29341 cgcccctgct accactgcga ttggcgcaac gggcagcttt atggcgctgc tggcggggat
    29401 ctctgccacc agttggtcgt ataccggcat ggcctctatc tgttatatga ccggcgaaat
    29461 taaaaacccc ggcaaaacca tgccacgagc gctgattggt tcctgtctgc tggttctggt
    29521 gctctacacc ctgctggcgc tggtgatttc cggcctgatg cccttcgaca aactcgccaa
    29581 ttctgaaacg ccgatttccg acgccctgac ctggatcccc gcactcggca gcaccgctgg
    29641 gatctttgtt gccatcacgg cgatgatcgt cattcttggt tcgctttcca gctgcgtgat
    29701 gtaccagccg cggctggaat acgcgatggc gaaagacaac ctgttcttca aatgcttcgg
    29761 ccatgtgcat ccgaaataca acacgccgga tgtctccatc attttgcaag gggcgctggg
    29821 gatcttcttc atcttcgttt ccgatctcac cagcctgctg ggctatttca gcctggtaat
    29881 gtgtttcaaa aataccctca ccttcggctc catcatctgg tgtcgtaaac gcgacgatta
    29941 caaaccgctg tggcgtactc cggctttcgg gctgatgacc cccctcgcca ttgcgtcaag
    30001 cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt cccggcctta tctgcgccgt
    30061 catcgttatt gccactggtc tgcctgctta cgccttctgg gcgaagcgta gccgccagct
    30121 caacgctttg tcgtaatgtt atctggagaa aataaaatgt tggatattga taaaagcact
    30181 gtggactttc tggtcaccga aaatatggtt caggaagtgg aaaaagttct cagccatgac
    30241 gttccgctgg tgcacaccat cgtggaagag atggtgaagc gcgacatcga tcgtatttat
    30301 ttcgttgcct gcggatcgcc actcaacgcg gcgcaaacgg cgaaacatct ggcagatcgc
    30361 ttttccgatc ttcaggttta cgccatttcc ggctgggagt tctgcgataa caccccatat
    30421 cgcctcgacg ctcgttgcgc ggtgattggc gtttctgact acggtaaaac cgaagaggta
    30481 atcaaagcgc tggagctggg ccgggcctgc ggcgcactca ctgcggcgtt caccaaacgc
    30541 gcggatagcc cgattacctc ggcggcggaa tttagcattg attatcaggc cgactgtatc
    30601 tgggaaattc atctgctgct ctgctacagc gtggtgctgg agatgatcac ccgcctcgcg
    30661 ccgcacgcgg aaatcggcaa gatcaaaaac gatctcaaac agctgccgaa tgcgctcggt
    30721 tatctggtac gcacttggga agaaaaaggc cgccagcttg gtgaactggc cagccagtgg
    30781 ccgatgattt acacggttgc tgcaggcccg ctgcgcccgc tgggttacaa agaaggcatc
    30841 gtgacgctga tggaatttac ctggacgcac ggctgtgtga ttgagagcgg tgagttccgt
    30901 cacggcccgc tggagattgt cgaaccgggc gttccgttcc tgttcctgct cggcaatgat
    30961 gaaagtcgcc acaccaccga acgcgccatt aactttgtta aacagcgtac tgacaacgtg
    31021 atcgtcatcg attacgccgc aatttcgcaa ggactgcacc cgtggctggc accgttcctg
    31081 atgttcgtgc caatggagtg gctctgctac tacctgtcta tttacaaaga tcacaacccg
    31141 gatgaacgcc gctattacgg tggtctggtg gaatattaat cctctctccc ggcccggcaa
    31201 cgggccggat ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc
    31261 agcggctgcc tattgaacat gcattccgtg atgcaagcga gctgggttac gatggcatcg
    31321 aaatttgggg cggccgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac
    31381 aaatcaaggc gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca
    31441 acggctatcc gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca
    31501 tgatcaagct ggcgatggat atggcaaaag agatgaacgc gggttatacg ctgatttctg
    31561 cggcccacgc gggctatctc acgccgccta acgttatctg gggcggctgg cagagaactt
    31621 aagcgaactg tgtgagtacg cggaaaacat cggcatggat ctgatcctcg aaccgttaac
    31681 gccgtatgaa tcgaacgtcg tgtgtaatgc taatgatgtg cttcatgcgc tggcgctggt
    31741 gccttcgccg cgcctgttca gcatggtcga catttgcgcg ccgtatgtcc aggcggaacc
    31801 ggtgatgagt tatttcgaca aactgggcga taaattacgt catctgcata ttgtcgacag
    31861 cgacggggcc agcgacacgc attacattcc cggcgaaggc aaaatgccgc tgcgggaact
    31921 gatgcgcgat attattgatc ggggctatga gggatactgc acagtggaac tggtaacgat
    31981 gtatatgaac gagcccagac tttacgcccg ccaggcgctg gaacgctttc gcgcgctgct
    32041 gccggaggat gagtgatgaa aaccctggcg acaatcggcg ataactgcgt cgatatctac
    32101 ccgcaactga ataaagcgtt ttctggcggt aatgcggtga acgtggcggt gtactgcact
    32161 cgctacggta taaagccggg atgcattacc tgggttggtg acgatgacta cggcacaaag
    32221 cttaagcagg atctcgccag catgggcgtc gatatcagcc atgtccatac gaaacacggc
    32281 gttaccgcac aaactcaggt ggaactgcac gacaatgatc gcgtttttgg cgactacacc
    32341 gaaggcgtga tggccgactt tgccctgagt gaagaggatt acgcctagct ggcgcagtat
    32401 gacattgtgc acgcggcaat ctggggacat gcggaagacg cattcccaca gctgcacgct
    32461 gcgggcaaac ttaccgcttt cgacgtctcc gacaagtggg acagcccgct ctggcagaca
    32521 ctggtgccgc atctcgattt tgcctttgcc tccgcaccgc aagaagacga agcgctgcgt
    32581 ctgaagatga aagcgattgt tgcccgtggc gcaggcgtgg tgattgtcac gttgggtgaa
    32641 aacggcagta ttgcctggga tggcgtgcag ttctggcgtc aggctcctga acaggtgacg
    32701 gttatcgaca ccatgggtgc cggagattcg ttcattgccg gattcctttg cggctggtct
    32761 gcggggatga cattaccgca ggcgatggcg cagggaacgg cgtgcgcggc acaaactatt
    32821 cagtaccacg gtgcctggta ggcataacgt tggcgtgagc atcttcacgc caacgtgctg
    32881 ttacttgccg gaaaacgacc ctataatccg tgtaattcat tctttatttc agggtcgatt
    32941 atgtcagcta cggaccgcta ctctcatcaa ctcctctacg ctaccgtccg ccagcggctg
    33001 ctggatgata tcgcgcaggg ggtttaccag gccgggcaac agatccctac cgaaaatgag
    33061 ctttgtacac aatataacgt cagccgcatt accattcgca aagccatcag cgacttagtg
    33121 gcagacggcg tactgatccg ctggcagggc aaaggcacct ttgtacaaag ccagaaggtc
    33181 gaaaacgccc tgctgaccgt cagtggtttt accgattttg gcgtctcaca aggcaaggcg
    33241 acgaaagaga aagtgatcga gcaagaacgg atcagcgccg cgccgttttg cgaaaagctc
    33301 aatatccccg gtaacagcga agtgttccat ctttgccggg tgatgtatct cgataaagag
    33361 ccgctattta ttgatagttc gtggatcccg ctgtcgcgtt atcctgactt tgatgagatt
    33421 tacgtcgaag gaagctccac ctatcagtta tttcaggagc gttttgacac gcgagtggtc
    33481 agcgacaaaa agaccatcga tatctttgcc gccacccgcc cgcaggcaaa atggctgaaa
    33541 tgcgaactgg gcgaaccgtt gtttcgcatc agcaaaatcg cctttgatca gaatgacaaa
    33601 ccggtgcacg tctccgaact cttctgccgc gccaaccgca tcaccttaac aattgataat
    33661 aaaagacatt aaccgtaggc cggataagat gcaccagcat cgcatccggc gatgccggcg
    33721 cactgaattt tacatcccgc acgttcccct caccctaacc ctctccccaa aggggcgagg
    33781 ggaccgtccg agcgttttga tattacggct tcgccacaaa accaatcgct tcgtacaccg
    33841 cttttagcgt acgggaagcg tgcgcgctgg ctttttctgc gccgtctttc atcacctgtt
    33901 gcaggaaggc ttcatcgttg cggaaacggt ggtaacgttc ctgcaattca gtcagcatac
    33961 cggaaacggc atcagccact tcgcctttca gatgaccata catcttgcct tcgaactgtt
    34021 tttccagttc cgggatgctc tggcccgtta ccgcagaaag gatatccagc aggttggaaa
    34081 cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg ctcgtcggag tcggtgaccg
    34141 cgcgtttgat tttcttcact accgatttcg gatcttccag caggccaata acgttattgc
    34201 ggttatcgtc agacttggac atcttcttgg tcggctccag cagcgacatt acgcgcgcgc
    34261 cggatttcgg aataaacggc tccggcacct taaagatatc gccgtacagc gcgttgaaac
    34321 gctgggcgat atcacggctc agttccaggt gctgtttctg gtcttcaccc accggtacca
    34381 gattagtttg atacagcagg atgtccgctg ccatcagcac cggatagtca aacagaccag
    34441 cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa ctgcgtcatg cggctcagtt
    34501 cgccgaagta ggtatagcag ttcagtgccc agcctaactg cgcatgttcc ggtacgtggg
    34561 actgaacaaa aatggtgctt ttctcaggat cgataccaca agccagatac aaggccagcg
    34621 tatccagcgt cgctttacgc agcttctgtg catcctggcg cacggtgatc gcgtgttggt
    34681 caacgataca gtaaatgcaa tggtagtcat cctgcatgtt tacccactga cgcagcgcac
    34741 ccatgtagtt accaatagtc aattcacctg agggctgtgc gccactaaaa acgatgggct
    34801 tagtcatttt tcgattcctg attttcgcta tgcggaagcc ctaatgcggg cagaaggtca
    34861 tttatagact gataaattac atcaggctgg ctgagatcga tagcctcgcc gtagttatat
    34921 ccgtaggtta agccaactga tgggcaacct gccgcttttg ctgcctgaat atcattgcgt
    34981 gagtcgccaa caaacagcat ctgttgtgga gcaattccca tccgctcagc caccagtaac
    35041 agcgggtccg gatgcggttt tttgttttgc acatcatcac caccaatcac cacgctgaag
    35101 tatttggcga tatctaaggc ttcgagcagc ggcgcgacga acggcgtcgg tttgttggtg
    35161 accaggccta gcggcaggcc tttagcctgc aacgcgccca acgtatcggc aacgtgcggg
    35221 aacaaaaacg tcccctcttc ggcaacctcg ccatagtaac gatcgaacag tttacgcaga
    35281 atacgtacct gttcttctgc cggaatgtcg tcatcaacgg gcggtttacc cattgtttta
    35341 cgcagagtcg cacgttcctg acgcgcccag gtcaatgcgc gctccatcag aacatctgcg
    35401 ccgttaccaa tccaggtaat aacgcgttct tcacctgcga cgggcaactc cagcgcatac
    35461 agcgccatat ctaccgcagc agcaagaccg ggagcactgt ccaccagcgt accgtcgaga
    35521 tcaaaagcga cgccgcgaat atcttcaaac ttactcatga cttacctttg ccagttcact
    35581 gcgcatttca tcaatgactt ttttgtagtc tggctggtcg aagattgccg aaccggcaac
    35641 gaacatatcc gcgcccgccg cggcgatttc gccaatgttg ttcaccttca cgccaccgtc
    35701 cacttccaga cgaatgtcaa agccagactc gtcgatacga cgacgtactt cgcgcagttt
    35761 atccagtgtt tgaggaatga aagattgacc gccgaaacca gggttgacgg acatcagcag
    35821 gatcacatcc agcttatcca tcacgtaatc cagatagctc agaggtgtcg ccgggttaaa
    35881 taccagaccc gctttacagc cattttcttt aatcagttgc agcgtgcggt caacatgctc
    35941 ggaggcttct ggatgaaagg taatgatgct ggcaccagcg gcagcgaaat caggcacaat
    36001 gcgatcgacc ggtttcacca tcaggtgtac gtcgataggg gcggtaatgc cgtagttacg
    36061 caaggatttc agcaccattg gcccaatcgt cagattggga acgtagtggt tatccatgac
    36121 gtcaaaatgc acgacatcag cgccagctgc cagggctttt gcggtatctt cacccaggcg
    36181 ggcaaaatca gccgacagaa ttgagggggc aatcaaatac tgtttcatcc gcttctcctt
    36241 gagaattatt ttttcgcggg tgaaacgact cctggtttgt acaaagccag cagttcgtcc
    36301 acctttttac gtgtgccgcc gttgctgctt atactgcgtc gaactttgac gacatgcaat
    36361 tttgcgcgct gataccactc acgcgttaac atcgtatcgt gattggagat cagcacagga
    36421 atatggcgct caaccagacc ttcggcgatc tccgccagat gcgcttgttg ttcaagcgta
    36481 aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag acagcggtgc ataaggtgga
    36541 tcgcaataga cgacagatgc atcatctgcg cgcgccatgc tatcggcgta agactcacaa
    36601 tagaaaaagg cattctgcgc tttttcagcg aagtgataca actctgcttc cgggaaatag
    36661 ggttttttgt agcggccaaa cggcacatta aactcaccgc gcagattgta acgacacagg
    36721 ccgttgtaac cgtagcggtt caaatataaa aacagtaccg cccgacggaa cggatcctgg
    36781 cttttgttga actcttcgcg gaactgatag taaacctcgg cgcaatttgt ttcgggaaca
    36841 aacagctcgc gtgcggcctg tacgtactca tcagtacgca tcttcacaat gttatagaga
    36901 ctgatcaggt cgctattgat atcggcaagg atataacgag aaaagtcggt gttgagaaac
    36961 accgacccgg cacctacaaa aggctcaacc agacattcgc ccttgggcaa atgccgttta
    37021 atatcatcaa gcaggggata cttgccccct gcccacttca aaaaagcgcg atttttcttc
    37081 atgctgacta actaattaca ccttctccgg ctgtggagaa agctccgaca gcatccagcg
    37141 cttcaggcaa ctcccgcaac gataaaccat tgcgggagat aaccttgatt acttcagatc
    37201 ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg gcctgaacat ctgctggcaa
    37261 tgtagatacc gcttttttcg cctcttcttt cgaagcgtac acgccagaaa ccaggacata
    37321 ccacggctga ccattacgcg tcgtttcata gacaacgtag tttttcagat tctctttctt
    37381 cgcccaaccg ttcaggttgt cgtagttaga ggaactgctc agctgcagag tgtaatggct
    37441 ggacggtgcc gatttcaacg aacccacatt acctgcggtc ttccctccag cggctggtgt
    37501 tgccgtggtt tgcgccgggg atgccgtctg tactggagcc gtagtcgccg tctcttttgg
    37561 tgctggcgta gaagtcgcag ccggtgcctt cgtgctcgcc acaggagcag ctggttcagt
    37621 acgcttcggc gtctgtgcta ccggcttcgg ttccgttttc acggttgctt gcggtttttt
    37681 cggttcaatc accgcctgct gacgagctgg acgcgtagtg gccggacgtt cagcagtttg
    37741 cgttttcgcc gtgtcacgcg atgcattgcc attgcgtact ggcgcgacag ttgctggttc
    37801 agtcggcaat gtagaattga ccgccacatt gttcaactgt tgctgatttt gtggctgggt
    37861 cagcgcattg ttcaggtcac cctgcacttc aacacgttgt tgaccatccg ttgccgccgg
    37921 ggtttgccct tgagtcggcg tagaagagat cggtggcaga gaaacatcct gctgagtatt
    37981 ttccgcagac gtggttcccg gcgctggctg cacgccattc gcctgatcgg tcgcattgcc
    38041 agcaagatca atactcttct cgccagacgc ggtttgatcg ctggaagtgg tcgagggggc
    38101 ttttagcgca gaaccgatac cgatgatcaa cagcagtaga accagaatgc cgacgcccat
    38161 catcatatac tgacgagaag cgggtttact ggctgctttt ttgcgcttac gcggacgacg
    38221 ctctacgcgc tcttcatcca cggtttcatc ttcggattcg tcaatttctt cttcgatttc
    38281 cggttcctca ttgcgctctt tttgcgcacg agtcggacgg cgatcgtcag tgtcatcaag
    38341 ttcaatatca tcaaaattga tctgcggttc gccacgttca gtacgctcag aacgttcaga
    38401 agattgacga gaacgaccag tacgacgatc gctgggatcg ggtttcagct cgtcttctgg
    38461 tttgaattca tccatttaac accccactaa aaggttaatg cttaccacgt tgcaattaac
    38521 ctgaagctaa tagaccgctt gataagcggc ctgacctttc ttgttgttac gctgattgac
    38581 aatcggcaat ggcgttaaga acaagctcgt gcgaaacgcc gctgcgaact tcactcttac
    38641 caattgccaa cggaagaatt aagcgcatct ctcccgcaag gactttcttg tcacgcagca
    38701 tatgcggtaa atacgcctgc gcggacattt cgcgcggccc attgaccggt aacccagccc
    38761 gcgtgagcag agttataata cgctgcgttt cggcagaact aaactgcccg agacgttccg
    38821 acgtccgcgc cgccatcacc atacccgcag cgaccgcttc accatgtaac caattgccat
    38881 accccatttc agcttcaatg gcatgaccaa aggtgtgtcc cagattcagt aaagcacgta
    38941 acccggtttc gcgctcgtcg gcggcgacaa cttctgcctt cagttcacaa caacggcgaa
    39001 tacagtacgc cattgccgga ccgtccagac gcaacaacgc atccagattc tcttccagcc
    39061 agttaaaaaa cgtaccgtca agaataatgc cgtatttgat gacttctgcc agccccgacg
    39121 ctaactcacg cgggggaagc gttttcagac agtcgagatc caccaccact gaagcaggct
    39181 ggtagaacgc gccaatcatg tttttaccga ggggatggtt gaccgcagtt ttgccgccaa
    39241 cggaggaatc gacctgcgac agtaacgtcg tcgggacttg aatgaaacga acaccgcgct
    39301 gataactcgc cgccgcgaag ccggtcagat cgcccactac gccgccgcca agcgccacca
    39361 gcgtagtatc gcgaccatgc ggcttttgta acaacgccgt aaagacggta tcgagtacag
    39421 ccaggctttt atactgctcg ccgtcaggaa ggataacgct atcgacgtta acacccgcct
    39481 gttcaagtac gccgcggacc ttatcgagat acagaggagc cagggtttcg ttggtgacca
    39541 acatgacctg ctcgcccgat ttcagcggta agaatgaagc tggttcatta aacaaaccag
    39601 atgcgatggt aattgggtaa ctacgttccc cgagagtaac gacaatcctc tccataacgc
    39661 gacatccacc ttaattactg tacccgcaga cgagtgtata taaagccaga attagttgct
    39721 ttccagcatg tgaataatct ggtttgcaac cactttagcg ctttgatcat cagtacgaat
    39781 ggtcacgtcg gcaatctctt catacagcgg attgcgttca ttggccaacg cttccagaac
    39841 ttcacgcggc ggtgtttcaa cgtgcagcaa cgggcgtttt ttatcacgct gcgtgcgtgc
    39901 aagttgcttt tcgatcgtcg tttcaagata aacgacaacg ccacgagcgg aaagacggtt
    39961 acgcgtttca cgggatttca cagagccgcc gccagtagcc agcacaatac cctgtttctc
    40021 ggtcaactca ttgatgacct tttcttcgcg atcgcggaag ccttcttcgc cttctaaatc
    40081 gaaaacccag cccacatcag ctccggttcg tttctcaatc tcttgatcgg aatcgtaaaa
    40141 ttccatattg agttgttgag ctaactggcg cccaatagtg ctttttccgg cacccatagg
    40201 cccaaccaga aagatattgc gtttctctgc cattttttcg gtactactaa gactattcgt
    40261 taatgataaa cccgcttcgc tcagagagct ccgcaggaca tgaactgaaa cctcataaga
    40321 tattgcgaga gtcagactga aaattatctc aatactccag cgggtttggc aactgaataa
    40381 atcacccatc cctctgcata tctggtcgct gcaagcgcgc tgccttgcta ccaccgctct
    40441 ggcgataaat caccgggtaa gattagcgta aaaaagacag caaaatgccg cctgaatgat
    40501 aaatcatcat catggaaggc aggcagcgaa gagcaccaac tctcaaatcg gtactccttg
    40561 tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt tcagcgtaaa aacattaccg
    40621 cttttacggc tgtttactca ctggaaacca gtcgtggcgt gataaacacc actaactcgc
    40681 gtcgttcatc ttcttttccg tcatgacgaa ataattgccc gaaccaggga atgtcgccaa
    40741 gcaacggcac gctatcctga cccgatttat ttttacgggt aaaaatgccg cccagcgcca
    40801 acgtttctcc gcttttgacc tcgacctgcg tttcgatctc ctgcttatca atcgccagca
    40861 cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt ctggctgatg tgtaatttca
    40921 gccggatgcg acctttttgt aacaccgtgg gcgtgacctc catccccagg acggcctctt
    40981 taaattccac cgacgtcgcg ccactttccc cgctggaaac ctgatatgga atttcgctcc
    41041 cctgtttaat gctggcaggc tgaagatgtg aggccagcag acgcggactg gcgataatat
    41101 ccagctgctg tttttgttcg agcgcggaaa gctcaagatc cagcaaacgt ccgttgatgc
    41161 gcccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac ggagaggtcg ctaccaagcg
    41221 tggtgacttg cccaacgcca ccagcgtgtt gcgcatcggc cagcgtccat ttcacgccta
    41281 actcacgcaa acttttttca ttaatggtga caatatgcgc cgacagctca acctgcccga
    41341 ccggcagatc catttgcgct acccactgtt caagggcgct taacgccgtt ttgttatctc
    41401 gtagcaaaag gcgattggtg cgtttatcga cggtcatact ccctttggca ctcagtagct
    41461 tctccccagc tttcgccagc tctccggcgt cggcgtattg cagagtaata ttgcgatttt
    41521 ccagcggcag atttgcctgc gcccgcgcct gctccgcttc ctgacgggcg atattgtcat
    41581 tctgccaggc aatggaatgc actgagagaa tgttgccctc ctggcgcgtt atcagtccgg
    41641 cgcttttcac tacagtttgt agtgcctgct tccagggaac atctgttaga tgtaacgaca
    41701 ccgtaccgct gacgtctggc gacaccacca ggttcaactt ctcctgttca gccagcgcct
    41761 gcaacacctg agctaccgga acgtcatcca ccatcagcgt cactttttgc ggctttgccg
    41821 cctgtacgcc gggtatcagc atcaacagta gtgcggctat ccattgcttc atttgtatct
    41881 ccttgccgtt gccacaacca ttgtggcggt tcgcagtttg tcccggtacc cagcgttagt
    41941 acgtctagcg tcagctgtaa aattgtccag ccgttttcca gcacatcgtt ttgctgcacc
    42001 cgtcgccatt tcttttgccc gtcttttatt acaccgatga tgcgctcgcc tcgccctacc
    42061 atcccctgat agcgccactg gctaagttcg ctaatccggc atagatcttc cggcggttta
    42121 aaagggtcac gcataccggt taaaaggcac aatgcaatac ctgccaacaa ccagcgttta
    42181 accctcatgc ggcgtctcca gttgtagggt gaacaaaaga tcatcacctt ccacgcttaa
    42241 tgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt gtaaatgccg acggcactgc
    42301 ttcccacagc gttttcaacg ccaactcgcc tccctgcgcg gatggatgcc agaaaaccag
    42361 ttgtgcgccg ggcaactgaa aatccagtgg cgaaaagggc agcgtttttt cttcgctgaa
    42421 gggagtggtg tcaaccaggc gatacagtgt ggcccaatgc tgatgatgac ttgcccgtag
    42481 tcgaattaat gcgtcgcgct cttcatggtg tgtcgatgac agaaaaatga gcgtaactaa
    42541 catcagcaac cagaatgccc agcaaaactg gcggaggcgg ggtgatgtgg cgaaccacca
    42601 gtcaaagaac atgttcatcg ctaaccttcc ttgttaactg atactcaaat tgccagcgtc
    42661 cctgcgcatc ctgctgcgtg gccccccgct gattgagatg aaaagatgca tcctggcgga
    42721 gtgacgtttc tagtgcgttt aacgcggtaa tgcttgttgt aagccccttg atctccagcg
    42781 ttccctgctg ccagcttatc gttgtcagcc aggcgtgctc gggtaaaagc gccgccagtg
    42841 cttccagcgc agattgccag tcgcgggtaa attgtcgctg gcgctgcctt tgcaaacgct
    42901 gctcacgaat ttgttggcgc tccagcaaac gtggtttggt tatctgtaag ctgcgggcga
    42961 gttgttgttc cccctgaagc aaaacggcgt ctacgcgagc ttcggcgctg cttgtcagac
    43021 gcagtattag cgttatcccg acggccagca gcagaggcgc aacgaacatc agcaaccaga
    43081 aacgaagaaa agcggtccgg cgttgctgtc gccagggcaa aaaattaatt ggcgggttca
    43141 tcagtacgcc ttcccaagcg ccagcccagc gcgatggcaa agtctccacc tggcggcggt
    43201 aacggcggct gacgaacaga aacggcctcc caaggatcaa atccgccttc gccacatatc
    43261 gcgacgcttt cgggatcaac ggataacgct gccgccagct ccttcgcgct agtcatcccc
    43321 accgccagtt tgcgccccca gcgatagcgc gtcgcccaca gccactgttc gttatcacgc
    43381 caggccagac attgctgatg agaaggtaaa aaaggcagga atcgctgtaa tgcactggca
    43441 tccggggtga tcgcactaac atgaacacgc aacctttctg ccaaagtaag cagcgttgcc
    43501 agctctttgc tttgcgcggc agtcacgtta taggcgggac tgagtgagtc ttcgctgtaa
    43561 tcgaagcgca gggagtccgg atccatatcc agctcgcgag ccatcgtccc ggacagccag
    43621 gccgtttgct cccgctcacc gagggacatc gacgggcgcg gaaatgaccg ctgtaatgtg
    43681 cgactggcgg gaaacgccaa catgatgtga tggcgctgcg gcagttcgcg actccacggt
    43741 aacaacgttt tagccagccg ctgcgcatca acaatccgcc catctttgat aatgtcgtgc
    43801 gccagcggca accgccacca gcgttgcaaa aagcattctt ttgcgccccg cacaatagca
    43861 accgctaccg cttcttgctg ttgtaaatgc aaaccaattt gccagatctt aaatgccatt
    43921 gtgatgatct ccttatcacc cgtcactctg acgggtatat caatgcgtct ggcttgcctt
    43981 tatactaccg cgcgtttgtt tataaactgc ccaaatgaaa ctaaatggga aatttccagt
    44041 gaagttcgta aagtattttt tgatccttgc agtctgttgc attctgctgg gagcaggctc
    44101 gatttatggc ctataccgct acatcgagcc acaactgccg gatgtggcga cattaaaaga
    44161 tgttcgcctg caaattccga tgcagattta cagcgccgat ggcgagctga ttgctcaata
    44221 cggtgagaaa cgtcgtattc cggttacgtt ggatcaaatc ccgccggaga tggtgaaagc
    44281 ctttatcgcg acggaagaca gccgcttcta cgagcatcac ggcgttgacc cggtggggat
    44341 cttccgtgcg gcaagtgtgg cgctgttctc cggccacgcg tcacaagggg caagtaccat
    44401 tacccagcag ctggcgagaa acttcttcct cagtccagaa cgcacgctga tgcgtaagat
    44461 taaggaagtc ttcctcgcga ttcgcattga acagttgctg acgaaagacg agatcctcga
    44521 gctttatctg aacaagattt accttggtta ccgcgcctat ggtgtcggtg ctgcggcaca
    44581 agtctatttc ggaaaaacgg tcgaccaact gacgctgaac gaaatggcgg tgatagccgg
    44641 gctgccgaaa gcgccttcca ccttcaaccc gctctactcg atggatcgtg ccgtcgcgcg
    44701 gcgtaacgtc gtgctatcgc ggatgctgga tgaaggatat atcacccagc aacagttcga
    44761 tcagacacgc actgaggcga ttaacgctaa ctatcacgcg ccggagattg ctttttctgc
    44821 gccgtacctg agcgaaatgg tgcgccagga gatgtataac cgttatggcg aaagtgccta
    44881 tgaagacggt tatcgcattt acaccaccat cacccgcaaa gtgcagcagg ccgcgcagca
    44941 ggcggtacgt aataacgtgc tggactacga catgcgccac ggctatcgcg gcccggcaaa
    45001 tgtgctgtgg aaagtgggcg agtcggcgtg ggataacaac aagattaccg atacgctgaa
    45061 ggcgctgccg acctatggtc cgctgctgcc tgccgcagtc accagcgcca atcctcagga
    45121 agcgacggca atgctggcgg acgggtcgac cgtcgcattg agtatggaag gcgttcgctg
    45181 ggcgcgtcct taccgttcgg atactcagca gggaccgacg ccgcgtaaag tgaccgatgt
    45241 tctgcaaacg ggtcagcaaa tctgggttcg tcaggttggc gatgcatggt ggctggcaca
    45301 agtgccggaa gtgaactcgg cactggtgtc gatcaatccg caaaatggtg ccgttatggc
    45361 gctggtcggt ggctttgatt tcaatcagag caagtttaac cgcgccaccc aggcactgcg
    45421 tcaggtgggt tccaacatca aaccgttcct ctacaccgcg gcgatggata aaggtctgac
    45481 gctggcaagt atgttgaacg atgtgccaat ttctcgctgg gacgcaggtg ccggttctga
    45541 ctggcagccg aaaaactcac caccgcagta tgccggtcca attcgcttac gtcaggggct
    45601 gggtcagtcg aaaaacgtgg tgatggtacg cgcaatgcgg gcgatgggcg tcgactacgc
    45661 tgcagaatat ctgcaacgct tcggcttccc ggcgcaaaac attgtccaca ccgaatcgct
    45721 ggcgctgggt tcagcgtcct tcaccccaat gcaggtggcg cgcggctacg cggtcatggc
    45781 gaacggcggc ttcctggtgg acccgtggtt tatcagcaaa attgaaaacg atcagggcgg
    45841 cgtgattttc gaagcgaaac cgaaagtagc ctgcccggaa tgcgatattc cggtgattta
    45901 cggtgatacg cagaaatcga acgtgctgga aaataacgat gttgaagatg tcgctatctc
    45961 ccgcgagcag cagaatgttt ctgtaccaat gccgcagctg gagcaggcaa atcaggcgtt
    46021 agtggcgaag actggcgcgc aggagtacgc accgcacgtc atcaacactc cgctggcatt
    46081 cctgattaag agtgctttga acaccaatat ctttggtgag ccaggctggc agggtaccgg
    46141 ctggcgtgca ggtcgtgatt tgcagcgtcg cgatatcggc gggaaaaccg gtaccactaa
    46201 cagttcgaaa gatgcgtggt tctcgggtta cggaccgggc gttgtgacct cggtctggat
    46261 tggctttgat gatcaccgtc gtaatctcgg tcatacaacg gcttccggag cgattaaaga
    46321 tcagatttca ggttacgaag gcggtgccaa gagtgcccag cctgcatggg acgcttatat
    46381 gaaagccgtt cttgaaggtg tgccggagca gccgctgacg ccgccgccgg gtattgtgac
    46441 ggtgaatatc gatcgcagca ccgggcagtt agctaatggt ggcaacagcc gcgaagagta
    46501 tttcatcgaa ggtacgcagc cgacacaaca ggcggtgcac gaggtgggaa cgaccattat
    46561 cgataatggc gaggcacagg aattgttctg attaaaaagg cgcttcggcg ccttttcagt
    46621 ttgctgacaa agtgcacttg tttatgccgg atacgggtga acgcgttatc cggctaacaa
    46681 aatcgtgaaa actcaatata ttgcaggaac cccataagcc tgataagcat tgtgcatcag
    46741 gcaaacttca cgcatttaca ctcgcccctg ccctttcaac cattcgcgca cgaggaacag
    46801 cgcgctgaca ttacgcgctt cgttgaagtc agggtcttcc agcaaatcca tcatatgcgc
    46861 cagcggccag cgcacctgtg gtagcggctc tggctcatcg ccttccagcg attccgggta
    46921 gagatcttgc gctaccacga tattcatttt gctggaaaag taagacggtg ccatgctgag
    46981 cttcttcaaa aaagtcagat cgttcgcacc aaatccaacc tcttctttta gctcgcggtt
    47041 agcggcttcg aagacgcttt cacccggatc aattaatcct ttcgaaaaac ctaattcgta
    47101 ggattcagtt cccactgcat attcgcggat caggatcagg tgatcgtcca caatcggcac
    47161 aatcatcact gcttcccggt tggttggacg cattcgttca taaacacgcc gcacgccatt
    47221 gctgaactct agatccacgc tctcgacggt aaacagtcgg gaacgggcta cagtttcaac
    47281 attcagaatg gtgggttttt gtaatgattt gctcatcgtg ggatctatgc tgtgaaatca
    47341 gcggttattg tgcgatatcg gacacgcttt cggcaatgtg aattgcatgt tatttacatt
    47401 tatgtaactt aataaataat cgtcctcaaa tcaaattaaa agtcaatagg ttgaaataac
    47461 tccaggaatt tgctgatatt ccgccttcag agggtttgct atgatcagcg gttactgtga
    47521 tgtgcttaat gatgctcaag ttaaactcca cgcttgccga tagccaaccg cagaatcatg
    47581 tattgtgtcc ggtgcgactg accacgcctg acagactaag taagatgggg aaagcatgag
    47641 caccattgtg atttttttag ctgctttgct ggcctgctca ctacttgcgg gatggctgat
    47701 aaaagtgcga tccagacggc gtcagctgcc ctggaccaac gccttcgcgg atgcgcaaac
    47761 gcgtaaactc acacctgaag aacgtagcgc cgttgaaaat tatcttgaga gcctgacgca
    47821 ggtattacag gtgcctggcc caacgggagc cagcgcggca ccgatctctc tggcgctgaa
    47881 tgccgaaagc aacaacgtca tgatgctgac acacgctatc acgcgttacg gcatctctac
    47941 cgacgatccg aataaatggc gttactacct cgattcggta gaagtccacc tgcccccttt
    48001 ctgggagcag tacatcaacg atgagaatac cgttgaactg attcataccg attcgctgcc
    48061 gctggttatt tcactcaacg gtcatacgct gcaggagtac atgcaggaaa ctcgcggcta
    48121 tgccttgcaa cctgttccgt ccacgcaggc gtcgattcgc ggggaagaga gtgagcaaat
    48181 cgagctactc aatattcgca aagaaacgca tgaagaatat gcgctgagtc gtccgcgcgg
    48241 gctgcgtgaa gcgttgctga tcgtcgcctc cttcctgatg ttctttttct gcctgattac
    48301 cccggatgta tttgttccgt ggctggcagg cggcgcgtta ctgctgctgg gcgcaggtct
    48361 gtgggggcta ttcgcccccc cggcaaaatc ctccctgcgg gaaattcatt gtctgcgcgg
    48421 tacaccccgt cgttggggat tgtttggcga aaacgatcag gaacagatca acaatatttc
    48481 actcggtatt atcgacctgg tctatcccgc acactggcag ccatacattg ctcaggatct
    48541 cggtcaacaa accgatattg atatctatct cgaccgccac ctagtgcgtc aggggcgata
    48601 tctttcgctg catgatgaag taaaaaactt tccgttacag cactggctgc gcagtacgat
    48661 tatcgctgcg ggctcgctgc tggtgctgtt tatgctgtta ttctggatcc cgctggatat
    48721 gccgctgaaa ttcactctct catggatgaa aggtgcgcag accattgaag ccaccagcgt
    48781 aaaacaactg gctgacgctg gcgtgcgggt aggcgataca ttgcgtatta gcggtacggg
    48841 aatgtgtaat attcgcactt ccggaacctg gagcgcgaaa accaattcac cttttttacc
    48901 gtttgactgc tcgcagatca tctggaacga cgcccgctca ttaccattac cagaatctga
    48961 actggtcaac aaagcgacgg cattgactga agcagttaat cgccagctgc accctaaacc
    49021 ggaagatgaa tctcgcgtca gcgcctcatt acgttcagca attcaaaaat ccggcatggt
    49081 attgcttgat gattttggcg acattgtact gaagacagcg gatctgtgtt ctgccaaaga
    49141 tgactgtgtg cgactgaaaa atgcgctggt caatctcggc aacagtaaag actgggacgc
    49201 gctggtaaaa cgcgccaacg ccgggaagct cgatggcgtg aatgtgttat tacgcccggt
    49261 gagtgcggaa tcgctggata acctggtggc aacctccacc gcgccgttta tcacgcatga
    49321 aacggcgcga gcggcacaat cactaaacag tccggccccc ggcggattcc tgattgtcag
    49381 cgacgaaggc agcgattttg ttgatcagcc ctggccttcg gcatcacttt acgactaccc
    49441 gccgcaagaa cagtggaacg ctttccagaa actggcacaa atgctgatgc atacgccgtt
    49501 taacgccgag ggtatcgtca caaaaatctt cactgacgcc aatggtacgc agcatatagg
    49561 ccttcatccg atcccggatc gttccggcct gtggcgctat ctcagcacca cgctgctgct
    49621 actgacgatg ctgggtagcg ccatttataa tggcgtacag gcctggcggc gttaccagcg
    49681 tcatcgcact cgcatgatga agattcaggc ctattatgaa agctgcctga acccgcaact
    49741 gatcacccct tcagaaagcc ttatcgaata acacgtttgc gcggcaggtt atgctaccct
    49801 gtcgcgcaaa ttgcttcact ctggagattt tcctcatgca tatcaacatt gcctggcagg
    49861 acgtagatac cgttctgctg gatatggacg gcacgttgct cgacctcgcc ttcgataact
    49921 atttctggca aaagctggtg cctgaaacat ggggcgcgaa aaacggggtt acgccacagg
    49981 aagcgatgga atatatgcgc cagcaatatc acgacgtaca gcatacgcta aactggtact
    50041 gtcttgatta ctggagtgag caactgggtc tggatatctg tgcgatgacc accgagatgg
    50101 gaccgcgtgc cgtactgcgt gaagatacca ttccgtttct tgaggcactg aaagccagcg
    50161 gtaagcagcg aattttgctc accaatgcgc atccgcacaa cctggcggta aaacttgagc
    50221 ataccggtct ggacgcacac cttgatttat tactttccac ccacacattt ggttatccga
    50281 aagaggatca gcggttatgg catgcggtgg ccgaagctac gggtctgaaa gctgaaagaa
    50341 cgctgtttat tgatgacagc gaagcgattc tcgatgctgc cgcgcaattt ggtattcgtt
    50401 actgcctcgg cgtgactaat cctgactcag ggattgccga gaaacagtat caacgccatc
    50461 cgtcactgaa tgactaccgc cgcctgatcc cctcgctaat gtgaaggaga cgccatgaaa
    50521 gaaaaacctg ctgttgaggt tcgactggat aaatggctat gggctgcccg tttttataaa
    50581 acccgcgcgc tggcccgtga aatgattgaa ggcggtaagg tgcattacaa cgggcagcgc
    50641 agcaagccga gcaaaatcgt cgagctgaat gccacgctca ctctgcgcca gggaaatgac
    50701 gaacgcacgg tgattgtaaa ggcgattact gaacagcgtc gccccgccag cgaggcagcc
    50761 ttgctgtatg aagagactgc ggaaagtgta gagaaacgcg aaaaaatggc gctggcacgt
    50821 aaacttaatg ccttaaccat gccgcacccg gaccgacgcc cggacaaaaa agagcgccgc
    50881 gacctgttac gatttaaaca cggcgacagt gaataactgt cacctgcaag agagatgatt
    50941 atgccgcaac atgaccaatt acatcgctat ctgtttgaaa actttgccgt gcgcggcgaa
    51001 ctggtaaccg tttcggaaac cctgcaacag atccttgaga atcacgatta tccgcagccc
    51061 gttaaaaacg tgctggcaga actgctggtt gcgaccagcc tgttaaccgc tacgctgaag
    51121 tttgatggtg atatcaccgt acagctgcaa ggcgacggtc cgatgaatct ggcggttatt
    51181 aacggtaaca acaaccagca gatgcgcggt gtggcgcgcg tgcagggcga aattccagaa
    51241 aatgccgacc tgaaaacgct ggtcggcaat ggttacgtgg tgatcaccat taccccgagc
    51301 gaaggtgaac gctatcaggg cgtggtgggt ctggaaggtg ataccctggc ggcctgcctg
    51361 gaagattact ttatgcgttc tgaacagctg ccgacgcgcc tgtttattcg caccggcgac
    51421 gtagacggca aaccggctgc aggcggtatg ttgttgcagg taatgcctgc gcaaaatgcc
    51481 cagcaggacg actttgacca cctggcgacg ctaaccgaaa ccatcaaaac cgaagaactg
    51541 ctgaccttac cggcaaacga agtgttgtgg cgtttgtatc acgaagaaga ggtgacggtt
    51601 tacgatccgc aggatgtgga gttcaaatgc acctgctcgc gtgaacgttg cgccgatgcg
    51661 ctgaaaacgc tgcctgatga agaagtcgat agcatcctgg cggaagatgg cgaaattgac
    51721 atgcattgtg attactgcgg taaccactat ctgttcaatg cgatggatat tgctgaaatc
    51781 cgcaacaacg cgtctccggc agatccgcaa gttcattaat gagtatgtcc ggcagagaga
    51841 gctctgccgg attttacgac taatacacct gatgcgcttt gtcactcatt cagattcaca
    51901 tccagccttt caccatccac tacaagatcg cgccacgctt taggcttcgt tcccagtttt
    51961 ccatccttcg cagctgtgag taatttttct ttcgttatat cacgcggaag taaactcatc
    52021 gtaagctctt gccattcttt cttgtcagga tcaaagccat agacaatgta tctttcatca
    52081 tcaaaggcaa agaggatccg ctctgccctg ccatcactat tcagatcttg ctcgaccagt
    52141 acgcaggcgt ctttttcaat acaggtcatc acgttatagc gatcctggat taaggccgac
    52201 cagaatgtcg catccggttt accagaacca ggggcaatta acacattttc ggctaatact
    52261 ttttccgata tctgttgctg aagatgttgc tctccatcta acgccatcag tagatcccgc
    52321 gcgcgtttcg ggtctttcat atactcggca tcgcttttca gcgactcaag cgccgcacgt
    52381 ccatagcgac cgctgtgttc gagcatgtag atagttacct ggtctggcgt gtttttgccg
    52441 ctctgataac gcgccatatg actgttcaca ctaatgcgca tactgtccag caccggcgaa
    52501 ttaagaagca ccagtattac caacaccaat aacgaaaccg caaggttcac tttgccctga
    52561 agaacaaggg gattttgccc tttacgccag acgatactga caaaataccc cagcgaccat
    52621 accagtaaca ccagcaccgc cagcgcgccc tgcaagcggt cgacagtcca gccgtactga
    52681 gcgacccgca gccataacgc ccaggcggcg atgaacacat acagcggagc aaccagcaga
    52741 gcggttttaa tcaggcaacg caacggcccc gtccagggaa gtgacgcttt ttgcggatcg
    52801 cggacaatag ccattaagat caattgcaaa aaggccagcg ttaacagcaa cccggcggcg
    52861 gagatgtggc gagaaatcgc actcaggcct gtaaacggca gggtgatgat aaacatcagg
    52921 gttagcaatg atactaacgg cagcaacccc gtggcgatta acgtgaacaa cttttgaata
    52981 gagtcgatta aacgtgactg tgttcgcgcg aggatcaccg ccagcgcggt aaccagacct
    53041 aacgtgagat aaataaacca gtcggttgca aaaaagagcg ttttgaaaaa cgtgatgcca
    53101 acaagtttaa acaactcact ccacagtaaa agcaccagcc acgtcagacc attggcgaga
    53161 aaaatcacca gtaatataag tacgttatgc catactgact ggtaaaaata gcgatagcgg
    53221 ctactgtcat tgcggatgcg aaggctttgt tgtatccacg gtagcaacaa cattgccatc
    53281 agcagcagat agcaaccaaa atcccaaagc gccttttcag ctctccaggg ggtcatgcca
    53341 tcagtttgcc acttcagcca gccgctcatc cccagcgtgg caataaacac cagcgccagc
    53401 catccccaaa ggcgcttctg tttaaaagag atcacggaga agagtaaaac ggatgaaaac
    53461 gcgacggtcg caggcacgcc ataaacaatc caactgtgat tttttccgga aagccaggcg
    53521 atcagcaggt agcaaaccag accctgaagt aatccggtag caatcatccc ccagcgtgtt
    53581 gcgggtgaaa gttcaacgtt atccatgttg gttatccaga atcaaaaggt gggttaatta
    53641 tcgcatccgg gcagtagtat tttgcttttt tcagaaaata atcaaaaaaa gttagcgtgg
    53701 tgaatcgata ctttaccggt tgaatttgca tcaatttcat tcaggaatgc gattccactc
    53761 acaatattcc cgccatataa accaagattt aaccttttga gaacattttc cacacctaaa
    53821 atgctatttc tgcgataata gcaaccgttt cgtgacagga atcacggagt tttttgtcaa
    53881 atatgaattt ctccagatac gtaaatctat gagccttgtc gcggttaaca cccccaaaaa
    53941 gactttacta ttcaggcaat acatattggc taaggagcag tgaaatgcgc gttaacaatg
    54001 gtttgacccc gcaagaactc gaggcttatg gtatcagtga cgtacatgat atcgtttaca
    54061 acccaagcta cgacctgctg tatcaggaag agctcgatcc gagcctgaca ggttatgagc
    54121 gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac cgggatcttc accggtcgtt
    54181 caccaaaaga taagtatatc gtccgtgacg ataccactcg cgatactttc tggtgggcag
    54241 acaaaggcaa aggtaagaac gataacaaac ctctctctcc ggaaacctgg caacatctga
    54301 aaggcctggt gaccaaacag ctttccggca aacgtctgtt cgttgtcgac gccttctgtg
    54361 gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac cgaagtggcc tggcaggcgc
    54421 attttgtcaa aaacatgttt attcgcccga gcgatgaaga gttggcaggt ttcaaaccag
    54481 actttatcgt tatgaacggc gcgaagtgca ctaacccgca gtggaaagaa cagggtctga
    54541 attccgaaaa cttcgtggct tttaacctga ccgagcgcat gcagctgatt ggcggcacct
    54601 ggtatggcgg cgaaatgaag aaagggatgt tctcgatgat gaactacctg ctgccgctga
    54661 aaggtatcgc ttctatgcac tgctccgcca acgttagcga gaaaggcgat gttgcggtgt
    54721 tcttcggcct ttccggcacc ggtaaaacca ccctttccac cgacccgaaa cgtcgcctga
    54781 ttggcgatga cgaacacggc tgggacgatg acggcgtgtt taacttcgaa ggcggctgct
    54841 acgcaaaaac tatcaagctg tcgaaagaag cggaacctga aatctacaac gctatccgtc
    54901 gcgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg cactatcgac tttgatgatg
    54961 gttcaaaaac cgagaacacc cgcgtttctt atccgatcta tcacatcgat aacatcgtta
    55021 agccggtttc aaaagcgggt cacgcaacga aagttatctt cctgaccgca gacgcctttg
    55081 gtgtgctgcc accggtttct cgtctgactg ccgatcaaac ccagtaccac ttcctctctg
    55141 gcttcaccgc caaactggcc ggtactgagc gtggcatcac cgaaccgacg ccaaccttct
    55201 ccgcttgctt cggcgcggca ttcctgtcgc tgcacccgac tcagtacgca gaagtgctgg
    55261 tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt taacactggc tggaacggca
    55321 ctggcaaacg tatctcgatt aaagataccc gcgccattat cgacgccatc ctcaacggtt
    55381 cgctggataa tgcagaaacc ttcactctgc cgatgtttaa cctggcgatc ccaaccgaac
    55441 tgccgggcgt agacacgaag attctcgatc cgcgtaacac ctacgcttcc ccggaacagt
    55501 ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga caactttgat aaatacaccg
    55561 acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa actgtaatga tttgaagctg
    55621 gagaatatct atccagtatc ttatagaaag caaaacggga ggcaccttcg cctcccgttt
    55681 atttaccctt cttttgtcgt gctctgcgcc cgcgttaccg gcacaggcag ccaggcgcga
    55741 atggaaagcc cgccccgctc gctggtgcca agctccagca tcccgttatg gttatccacg
    55801 atacgctgca caatcgccag ccctaatccc gtgccgctaa tggtgcgtgc actgtcgccg
    55861 cgaacaaacg gctggaaaag gtgcttacgt tgttccggcg caattcccgg accgtcatct
    55921 tccacctgga accaggcgcg attcggctcc gtcccgctgc tgactttgat ccagccattg
    55981 ccgtaacggg cggcgttgac caccatattc gccaccgcgc gtttgatcga cagcgggtgc
    56041 attttcactt caatgctgcc ggggtaaagc gcggtttcaa tttcccgctc atagccactt
    56101 tcggcagcaa taacctcacc gagtactgcg ttgagatccg ccatttccat cggcatctcc
    56161 tgtccggtgc gcaggtagtc gataaactgc tcaatgatgg cattgcactc ctcgatatct
    56221 ttattgatcg attctgccag gtagccatcc tgctcgctca tcatctcggt cgccaggcga
    56281 atacgcgtca gcggtgtacg caagtcgtga cttacccccg ccatcagcag cgtgcggtca
    56341 tccgccagtt gcttaacacc agccgccata tggttaaagg cgcgggtaac ggaacgcacc
    56401 tcggacgcgc catactcacg cagcggcggc ggaataatcc ctttaccaac ctgcaaggct
    56461 gcgtgttcga gatcgaccaa cggtcgattc tggatacgaa taaacagcca cgccccgcct
    56521 atcgccaata gcataatcgc caacgtatag cggaacagcg gagagaaatc gccctgatga
    56581 atttcggtca gcggcacgcg tacccagata ttgggcgaca gccaggtttt cagccagacg
    56641 acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg ggccgcccag ttgctgtgcc
    56701 atctgatggc ttaagaattc atagtgttgc gcccaacgca gacctgcctc ttcggcagcc
    56761 tcgttggagt agagagagat ccccagctca cggtagatct cccgacggaa agcgggaggc
    56821 acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg tcatcaacat acgcacttcg
    56881 tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa tcgcgaagtt cagcaccacc
    56941 agataagtcg tcaccaggct ggcgaacagc aaggtgacga tgagcaataa cgtacgggca
    57001 aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt agagccgtcc ggtacaaaga
    57061 cgtagcccag gccccaaacg gtctgaatgt aacgcggatg cgctggatct tcttccacca
    57121 tgcggcgcag acgagaaatc tgcacgtcga tggagcgttc cattgcggaa tattcacgac
    57181 cacgggcaag gttcatcagc ttatcgcggg agagcggctc acgcggatgg ctgaccagtg
    57241 ccttcagtac cgcaaactca ccgctggtga gcggcatcgg ctcgtcttcg cggaacattt
    57301 cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat taccgcctct tcctgtgacg
    57361 gtgcgcctgg tagctcgttc gcctgacgac gcagcaccgc acggatacgg gccagcagtt
    57421 cacgcgggtt aaacggtttt ggaatgtaat cgtcagcgcc aatctccagg ccaacgatac
    57481 ggtccacttc ttcccctttc gccgtcacca taatgatcgg catcgggttg ctctgactac
    57541 gaagacgtcg gcaaatcgac aagccatctt caccaggtaa cattaaatcc agtaccataa
    57601 gatggaaaga ttcacgagtc agcaggcgat ccatctgttc tgcattagcg acgcttcgaa
    57661 cctggaagcc ttgttcggtg agataacgtt ccagcagcgc acgcaggcgc atgtcgtcat
    57721 cgaccaccag aatcttgtag ttctcttgca ttgtttgtac tcccaaaggt tcgcaacaat
    57781 ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc agctaaatct ggtatgaatt
    57841 tcagcctaaa ttgttacaaa gcatattaaa cagcagctta agtatacaat ttattcggcg
    57901 aaacattatt gattctgttg atatgatcac gttataccca atgtgcgcat tatcaaacag
    57961 acaaagggaa tcaacgagat gaaaacgccc ctggttaccc gggaagggta tgaaaaactc
    58021 aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg aggtcacaaa aaaggtgacc
    58081 tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact atcagtataa taaaaagcgc
    58141 ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat gcatggaaaa tctcaaaatc
    58201 gtcgattact ccccgcagca ggaaggcaaa gtcttttttg gcgcgtgggt ggagattgaa
    58261 aacgacgatg gcgtgactca ccgtttccgt attgtcggct acgatgaaat ttttggccgt
    58321 aaagattaca tctctatcga ttccccgatg gcccgcgcat tgctgaaaaa agaagtcggc
    58381 gatctggctg tggtgaatac tcctgccggg gaagcgagct ggtatgttaa tgctatcgag
    58441 tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat ggctggcatt tttgccagcc
    58501 agtccgtata actatcccct gatttttgat ccgaaaagat gaactcaaac catgatgaat
    58561 gattcgttct gccgcattat tgcgggtgaa attcaggcgc gcccggaaca ggttgacgct
    58621 gctgttcgcc tgcttgacga agggaatacc gtgcctttta ttgcacgtta tcgtaaggaa
    58681 atcaccggcg gtctggatga cacgcagctg cgtaatctgg aaacgcgtct gggatacctg
    58741 cgcgagctgg aagagagacg tcaggcgatc ctcaagtcca tttccgagca aggcaaactc
    58801 accgatgatc tggcgaatgc catcaacgcc accctaagca aaaccgaact cgaagacctc
    58861 tacctgccct acaaacctaa acgccgcacc cgcgggcaaa tcgccattga agcagggctt
    58921 gagccgttgg ctgacctgct gtggagcgat ccgtcacaca cgccagaagt cgccgctgcg
    58981 caatatattg atgccgataa aggcgtggca gataccaaag ccgcgctgga cggcgcgcgc
    59041 tatatcctga tggaacggtt tgccgaagat gccgcgctgc tggcgaaagt gcgtgattat
    59101 ctgtggaaga acgcgcattt ggtttctacg gtggtgagcg gtaaagaaga ggaaggggcg
    59161 aaattccgcg actatttcga tcatcacgaa ccgttgtcca cggtgccttc tcaccgcgcg
    59221 ctggcgatgt tccgtgggcg taacgaaggc gtacttcagc tttcgctgaa tgccgatcca
    59281 cagttcgatg agccgcccaa agagagctat tgcgagcaaa tcatcatgga tcaccttggc
    59341 ctgcgcctga acaatgcccc ggcggatagc tggcgcaaag gcgtggtgag ctggacgtgg
    59401 cgcatcaagg tgctgatgca tctggaaacc gaactgatgg gcaccgtgcg cgaacgtgcg
    59461 gaaaatgaag caatcaacgt ctttgcccgt aacctgcacg atctgctgat ggctgcccct
    59521 gccggactgc gtgcaacgat gggcctcgat ccgggtctgc gtaccggggt aaaagtggcg
    59581 gtggtcgatg ccactggcaa actggtagcg accgatacca tttacccgca caccggacaa
    59641 gccgcaaaag cagcgatgac tgttgctgcg ctgtgtgaaa agcataacgt tgaactggtg
    59701 gcgatcggca acggtacagc ttcccgcgaa accgaacgtt tctatctcga cgtgcagaag
    59761 cagttcccga aagtgaccgc acagaaagtg atcgtcagcg aagctggcgc gtcggtttac
    59821 tcggcttccg agctggcagc gcaggagttc ccggatctcg acgtttcgct gcgtggcgcg
    59881 gtttctatcg cccgccgttt gcaggatccg ctggcggagc tggtgaaaat cgatccgaaa
    59941 tctatcggcg taggtcagta tcagcatgac gtcagccaga cgcaactggc ccgcaaactg
    60001 gatgcagtag tagaagactg cgtaaacgcc gttggcgtcg atctcaacac cgcttctgtt
    60061 ccgctattaa ctcgcgtggc gggcctgacg cgaatgatgg cgcaaaacat cgtggcctgg
    60121 cgcgatgaga acggccagtt ccagaaccgt cagcaactgc tgaaagtcag ccgtctgggg
    60181 ccgaaagcct tcgagcagtg cgcgggcttc ctgcgcatta accacggtga caacccgctg
    60241 gacgcctcta ccgttcaccc ggaagcctat ccggtggtgg aacgcattct ggcagcaaca
    60301 cagcaggcac tgaaagatct gatgggtaac agcagcgaac tgcgtaacct gaaagcgtct
    60361 gactttactg atgaaaaatt cggtgtgccg acagtaactg acatcatcaa agagctggaa
    60421 aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc agtttgccga tggcgtcgag
    60481 acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg ctgtcaccaa cgtcaccaac
    60541 tttggcgcgt ttgtcgatat cggcgtgcat caggacggcc tggttcacat ctcttcattg
    60601 tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag cgggcgacat tgtgaaggtg
    60661 aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc tgactatgcg tctggacgag
    60721 cagcctggcg aaaccaactc tcgtcgcggc ggcggtaacg atcgcccgca aaacaaccgc
    60781 ccggcagcca aaccacgcgg tcgtgaagcg cagcctgccg gtaacagcgc gatgatggac
    60841 gcgctggcgg cagcaatggg caaaaaacgt taaacgcccg gactggcccc taaacctgcg
    60901 cgataaggcg ttcacgccgc atctggcaac gaacgccttg cctgacataa aagtgccgga
    60961 gaatatctcc ggcatattta ttccacagcc aaactcacaa tatatttcgt taatatttat
    61021 cacttcatta acaactgaaa ccttaattaa acattagcca gtcctggtaa ttcgctattc
    61081 gaattatatt ttcgctgcga tataaccttg agccacatca acattgagtc agattattat
    61141 tcaaaccaac attcgcacac attttaagta ttgctgatag aaaccattct cattatcatt
    61201 gtactattga ttatttaatc tctccttcgt tggcaaatca tctggtctca tgtcgctgtc
    61261 aaacgcccca tgaggtagtt atccagttaa tgagaaacaa gtaggcacct atgcaataca
    61321 ctccagatac tgcgtggaaa atcactggct tttcccgtga aatcagcccg gcatatcgcc
    61381 aaaaactgct ttctcttggc atgttacctg gctcctcttt taatgtggtg cgcgtcgctc
    61441 cactcggcga ccccattcat atcgaaaccc gtcgtgtgag cctggtatta cgcaaaaaag
    61501 atctggcctt attagaagtg gaagcggttt cctgttaata cggtgataac aacaatgaaa
    61561 aaattaacca taggcttaat tggtaatcca aattctggca agacaacctt atttaaccag
    61621 ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg ttaccgtcga acgtaaagaa
    61681 gggcaattct ccaccaccga tcatcaggtc acgctggtgg acctgcccgg cacctattct
    61741 ctgaccacca tctcatcgca gacctcgctc gatgagcaaa tcgcctgtca ctacattttg
    61801 agtggcgacg ccgacctgct gattaacgtg gtggatgcgt ctaaccttga gcgtaacctg
    61861 tacctgacgc tacaactgct ggaactcggc attccctgca tcgtggcact gaacatgctc
    61921 gacattgccg agaagcaaaa tattcgtatt gaaattgatg ctctgtcggc gcgtcttggc
    61981 tgcccagtga tcccgctggt ttcaacccgt ggtcgcggta ttgaagcgct caagctggcg
    62041 attgatcgct ataaagctaa cgagaatgtg gaactggtgc attacgcaca gccgctactc
    62101 aacgaagcag attcactggc aaaagtgatg ccttccgaca tcccgctgaa acaacgtcgc
    62161 tggctgggcc tgcaaatgct ggaaggcgat atctacagcc gcgcctacgc cggtgaagcg
    62221 tcgcagcatc ttgatgccgc cctcgcccgt ctgcgtaatg agatggacga tccggcgctg
    62281 cacattgccg atgcgcgtta ccagtgcatt gctgccatct gtgatgtggt aagcaacacc
    62341 ctgacggcag aacccagccg tttcaccact gcggtagata aaatcgtgct caaccgtttc
    62401 ctcggtctgc cgattttcct ctttgtgatg tacctgatgt tcctgctggc tatcaacatc
    62461 ggcggggcgt tacagccgct gtttgacgtc ggctccgtgg ctctatttgt gcatggtatt
    62521 caatggattg gctacacgct ccacttcccg gactggctga ctatcttcct cgcccagggc
    62581 ctgggtggcg gtattaacac cgtgctgcca ctggtgccgc agatcggcat gatgtacctg
    62641 ttcctctcct tccttgagga ctccggctat atggcgcgtg cggcgtttgt gatggaccgc
    62701 ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc cgctgatcgt cggtttcggt
    62761 tgtaacgtac cgtcggtaat gggtgcacgt acgcttgatg caccgcgtga acgtctgatg
    62821 accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc tggctatctt cgcagtattc
    62881 gcggctgcct tcttcgggca gaacggtgcg ctggcggtct tctcgctgta tatgctcggt
    62941 atcgtgatgg cggtgctgac tggcctgatg ctcaagtaca ccatcatgcg cggtgaagcg
    63001 acgccgtttg tcatggagct gccggtctac catgtaccac acgttaaaag cctgattatc
    63061 cagacctggc agcgtctgaa aggcttcgtt ctgcgtgctg gtaaagtgat tatcatcgtc
    63121 agcattttcc tgagcgcttt caacagcttc tcgttgagcg ggaaaatcgt cgataacatc
    63181 aacgactcgg cgctggcttc cgtcagccgg gtgatcaccc cggtcttcaa gccgattggc
    63241 gtgcatgaag ataactggca ggcaacagtt ggcctgttta cgggtgcaat ggcgaaagaa
    63301 gtggtggtgg gtacgctcaa caccctctac accgcagaaa atattcagga cgaagagttc
    63361 aatccggcgg aatttaacct cggtgaagag ttgttcagcg cggtagatga aacctggcag
    63421 agcctgaaag acaccttcag ccttagcgta ctgatgaacc ccattgaagc tagcaaaggc
    63481 gacggcgaaa tgggtaccgg ggcgatgggc gtgatggatc agaaattcgg tagcgcagct
    63541 gccgcataca gctacctgat tttcgtcctg ctgtatgtac catgtatctc ggtgatggga
    63601 gccatcgccc gtgaatcaag ccgtggctgg atgggcttct ccatcctgtg ggggctgaat
    63661 atcgcttact cactggcaac attgttctat caagtcgcca gctacagtca gcacccaact
    63721 tacagcctgg tgtgcattct ggcggttatc ctgtttaaca tcgtggttat cggtctgctg
    63781 cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa cccgcaagtc ggtaagtagt
    63841 tgctgcgcag ccagcaccac cggtgattgc cattaatggc ttcacttatt caggtgcgtg
    63901 atttgctggc gttacggggc cgtatggaag cggcccagat aagccagaca ttgaacactc
    63961 cacagccaat gattaacgcc atgctgcaac aactggaaag tatgggcaaa gccgtgcgga
    64021 ttcaggaaga acctgacggc tgcctctccg gcagttgtaa aagctgcccg gaaggaaaag
    64081 cctgtctgcg cgagtggtgg gcgctgcgtt aaccatactc catcgcctgt tttgaaaagc
    64141 ccggtatgcg tttgcatccg ggcttttttg cgtgcggctt tccataaaaa tgcaattctt
    64201 gcagcgcgac gtaagttcct ctgaaagcgt ctcgcaggga tgaaaactgg ctaatgcaca
    64261 ggtgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca ggtgaaggaa cgccatgagc
    64321 aaaaagcaga gttccacccc acacgatgcg ctgttcaaac tctttttacg ccaaccggag
    64381 acggcacgcg attttcttgc gtttcattta ccagcaccca ttcacgcgct ctgtgatatg
    64441 aaaaccctca agctggagtc gagcagcttt attgatgacg atctgcgtga aagctattcc
    64501 gatgtgctgt ggtcggtgaa aacagaacaa ggaccaggat acatctattg tctgattgaa
    64561 catcaaagca cctcaaacaa actgatcgca tttcgcatga tgcgttacgc tattgccgca
    64621 atgcaaaatc accttgatgc cggatacaaa acgttgccga tggtggtgcc attgttgttt
    64681 taccacggta ttgaaagccc ctatccctat tcgctgtgtt ggctggattg tttcgccgat
    64741 cccaaactgg caaggcagct ttatgcctcc gcatttccgc tgattgatat caccgtcatg
    64801 cctgatgatg aaatcatgca gcaccgacgc atgacgctgc tggagttaat tcaaaaacat
    64861 attcgtcaac gcgacctgat ggggctggta gagcaaatgg cctgcttatt aagtagtgga
    64921 tacgctaatg acagacaaat caaagggctg tttaattaca tactgcaaac cggcgatgcg
    64981 gtacgtttta acgattttat cgacggcgtt gccgaacgat caccgaaaca caaggagagt
    65041 ttaatgacta ttgcggaaag attgcggcag gagggggaac aatccaaagc cctgcatata
    65101 gccaaaataa tgcttgaatc cggagtccct cttgcagaca tcatgcgctt taccgggctg
    65161 tcagaagaag agttggctgc ggcgagtcag taaagttctg tctcgccatt caaaagtgcc
    65221 tacaccctct gcttcaacgc caccagcacg tgacgaaact cgaccggatg cgaaataaac
    65281 ggcgcatgag ccgctttggc gaagatgtat gactcgctgt gtggccagag tttatccagc
    65341 atcggcacca ctttgcgcgg caccagacca tcgagatagc catacagtcg caaaaacggc
    65401 atgggtacgt tttgcagcgg caagcggaga tcaaccgttt tgaggatttc cagcccacca
    65461 ttgagcacgt caacctccgg catcggtaac gccagaacgg ttttcttcaa cgcccgcgca
    65521 tcctgacgcg ccgtttcagt acccatggtt tgtaacgcaa ggaaccgttc caccgtacgc
    65581 tgaaaatcat cactgagttg ctgctgaaat cccgccagca cgtccggttt tatccccggc
    65641 cactcgtcac gagcactaaa acaaggtgac gacgccacgg tgaccagcgc ctgaacacgc
    65701 tcgggatggg ttaacgcaat ctggcttgcc accagcccgc ccagactcca gcctaaccaa
    65761 atggctttat caggtgcctg tcgcagcacg gcttcggcca tctccgcaag tgacagcgca
    65821 ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa gatgcagcgt aaaatgcgag
    65881 ctaagttcct cgtcaatgca acgccacact tcagcattca gtccccatcc gtgcagcagc
    65941 acaagatgaa cattcccctg acctttggtc tgccaccaga tgttattcat ccgctattgt
    66001 tctcttttga cttacaagga tgaacatatg ctaacagtac cgggattatg ctggctatgc
    66061 cgaatgccac tggcattagg tcattggggg atttgttcgg tctgctcacg cgccgcccgt
    66121 acagataaaa cgttatgccc acaatgtgga ttacccgcca cacactccca tcttccctgc
    66181 ggtcgctgcc tgcaaaaacc gccgccctgg caaagactgg tcacggttgc cgactatgcg
    66241 ccgccgttaa gtctgctcat ccaccagctt aagttttccc ggcgcagcga aatcgccagc
    66301 gccctgtcac gtctgttact attggaagtc ttacacgctc gtcgcaccac cgggttgcaa
    66361 ttgccggatc gtatcatcag cgttccgtta tggcagcggc gtcactggcg tcggggattt
    66421 aatcagagcg atttgctgtg tcagccgtta tcacgctggt tgcactgcca atgggatagc
    66481 gaagccatca cacgtacacg ggccactgcg acccagcatt ttcttagcgc ccggctacgc
    66541 aggcgcaacc tgaaaaatgc ctttcgcctt gaattgcctg tgcaaggtcg ccatatggtg
    66601 attgtggatg atgtcgttac caccggaagt accgtcgcag agattgcgca gttgctttta
    66661 cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc gaaccttgta gagcctcgat
    66721 gatgggcgta ttataaccaa ctaaaatagt caactattag gccattacta tgatccgtat
    66781 ttccgatgct gcacaagcgc actttgccaa actgctggca aatcaggaag aagggacaca
    66841 aatccgcgta tttgtgatta accctggcac gcctaacgct gaatgtggtg tttcttattg
    66901 tccgccggat gccgtggaag ccaccgacac cgccctgaaa tttgacctgc tgaccgcgta
    66961 tgttgatgag ttaagcgcac catacctgga agatgcagag atcgattttg ttaccgacca
    67021 gttgggttct cagctaacac tgaaagcgcc taacgccaaa atgcgtaaag tggcagacga
    67081 tgcaccactg atggagcgcg tggagtatat gctgcagtcg cagatcaacc cacagcttgc
    67141 tggtcacggt ggccgcgttt cactgatgga aatcaccgaa gacggttacg ccattctgca
    67201 atttggcggc ggctgtaacg gttgttccat ggtcgatgtg acgctgaaag aagggatcga
    67261 gaagcagcta ctaaacgaat tcccggagct gaaaggtgtg cgcgatctca ccgaacacca
    67321 gcgcggcgaa cactcctact actaagttat cccctctttt gtagattgcc cgatgcgacg
    67381 ctaaagcgtc atttcgggcc tacgttgaac atgtgccaga cgttggcgca gcattgcttt
    67441 ggcaaatccc tcccttcgtt tccccgcgat aatatgacca acctctcata atttaaattt
    67501 accccgctct ggtgattctc aaacgccaga tgttacccgt atcattcacg tgggtaccaa
    67561 acatactcct gacatctgac tacaataatt agttttagtg ggtatcagtc gtggtgccgc
    67621 aatatctctg ttcccgattg ggataattag agcttgtcgt cagaaaattg acgttaccca
    67681 taacaaatga aaggccaggt aaatcatgcc attagtcatt gttgctatcg gtgtaatctt
    67741 gttattactc ctgatgatcc gcttcaaaat gaacggcttc atcgctctcg tcctcgtggc
    67801 gcttgcggtt ggattaatgc aagggatgcc gctggataaa gttattggct ccatcaaagc
    67861 cggtgtcggc gggacgctcg gtagccttgc cctgatcatg ggttttggcg caatgctggg
    67921 caaaatgctg gcagactgcg gtggcgcaca acgtatcgcc accacgctga ttgccaaatt
    67981 tggtaaaaaa cacatccagt gggcggtggt actgaccggt tttaccgttg gttttgccct
    68041 gttctatgaa gtgggctttg tgctgatgct gccgctggtg tttaccatcg cggcttctgc
    68101 gaatattcca ctgctgtatg ttggtgtacc aatggcagct gcactgtctg tgacccacgg
    68161 cttcctgcca ccgcatccgg gtccgactgc gattgccacc attttcaatg ccgatatggg
    68221 taaaaccctg ctgtacggta ctattctggc aatcccgacc gtgattctcg ccggtccggt
    68281 ttacgctcgc gtgttgaaag gtatcgataa gccaattccg gaaggtctct acagcgcgaa
    68341 aaccttcagc gaagaagaga tgccgagctt tggcgtcagc gtctggacct ctctggtgcc
    68401 ggtagtactg atggcgatgc gtgcgattgc cgaaatgatc ctgccgaaag gtcacgcttt
    68461 cctgccggta gcggagttcc tcggtgaccc ggtaatggct acgctgattg ccgtgctgat
    68521 tgcgatgttc acctttggtc tgaaccgtgg tcgttcaatg gatcagatta acgacacgct
    68581 ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc atcggtggtg gcggtgcgtt
    68641 caagcaggtg ctggtagaca gcggcgtgga caaatacatt gcttccatga tgcacgaaac
    68701 caacatttct ccgctgctga tggcctggtc gattgctgcc gtactgcgta tcgcgctggg
    68761 ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg gcaccgctga ttgcaacgac
    68821 gggtgttagc ccggagctga tggttattgc ggtgggttcc ggtagtgtga ttttctctca
    68881 tgtgaacgat ccgggcttct ggctgttcaa agagtacttt aacctgacta tcggcgagac
    68941 catcaaatcc tggtcgatgc tggaaaccat tatctcggtg tgcggtctgg taggctgtct
    69001 gctgctgaat atggtgattt gataacactg actgccggat gcatcgcatc cggcatcaac
    69061 cgcactctac ttcttcttcg ctgcagctct gcgccgtctg tccaaatcct tcagcaactt
    69121 gttcacgcca tcatcggcaa acatcgtctc aagcgttgcg gaaagcttgc gtcgccagtt
    69181 tttatactgg taactggtgc caggaatatt caccggttcg gccatatcca gccagtcttc
    69241 cggctgtagt cctaacagag cactgttact gtcggcaatg tagcgctgca aaccacgatt
    69301 aagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg gcagacaacc
    69361 atatttatgc agtgcatcca gcagcccttg cttcgccagt tcgcgatcct gatacagacc
    69421 gcgcagtacc acttcatccg gatacagccc cagggttttg cccagcgtta gatccccgct
    69481 ctcccagtag ccgcgaagcg taggcaggtc atgtgtcgcc gcaaccgcca tcgactgctc
    69541 cggatacgct ttcggtgcac ggaacgtctt ctcgtggtcg ttttcgaaat agagcacttt
    69601 gtaggagtac acaccgctgc tgcgcagctt accgacaatc tctaccggta cggtaccgag
    69661 atcttcacca atcaccatac agcgatgacg tttactttcg agtgccagaa tcgagagcag
    69721 atcatccacc ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat acggtatcca
    69781 ccacaaacgc agcatcgaca tcacatggtc aattcgtaat gcgccgcagt tttgcatatt
    69841 ggcacgcaac agctcgataa acggttcata agcacgcgcg gtgatgatat gcgggtccat
    69901 tggcggcaat ccccagttct gccccaacgg gccgaggata tccggcggcg cgccaaccga
    69961 cgctttcagg caatacagtt cacggtcgca ccaggtttcc gccccacctt ccgctacgcc
    70021 aaccgccaga tcacgataca agccaatcgg catttcatag ccctggctta tctcccagca
    70081 ggcggcaaac tggctgtaag cctgccactg caaccagaga taaaaatcaa cgtcatcgcg
    70141 atgttcttcg cagaactgac gcacttctgg tgaatccacg ttctgataca tctctggcca
    70201 tgcaggccag ccccagcgca tttcgtcctc tttcacttgc tgggcatgta gcgcatcaaa
    70261 ggctgcctgc cagaacaggc tgtcgccttg atctgcaaca aactggcgaa acgcggccat
    70321 ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg tcatttttag
    70381 ggcggtaacc gtggagtaat cgacccagtc ggcatcccgc gcctgttgca gcgtctgttg
    70441 cgtggtcggc aactgccacc aggcctgagc ctcttcgcta agatggaaat cttcgacggc
    70501 gttaacgtcg atataaatca cattcagcca gcggcgagaa gacgggctat acggactggc
    70561 gctctccgga tttgccggat agagcgcatg aatcgggttc agaccaatga acgatccgcc
    70621 acgttttgcc acatccacta gcatcgcttt gagatcgcca aaatccccaa taccccagtt
    70681 tttttccgat cgcagcgtat aaagctggac gcaggcaccc cacagctttt gtttattcag
    70741 caacgcctgc ggttcgtaac agcgtttcgg ggcgacaatc accctgcaat gcccgcgctg
    70801 gtcttcctgg gtaagcgtca gcgtgtgata accttccggc agcttcgtcg gtagattgaa
    70861 cgctttgccc cccgttatat ggcctttgta ctgcgttcct tcttcggtgg tcagcagcca
    70921 gctatattcg ccgctgccct ccaccaccat cggcattttt ttgccgctgg tataaaccat
    70981 gacattcggg actggcgtta ccgccacttt cgtggcggta cgttgatgca tcgcgtcaag
    71041 caaacgccgt ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat tgatgtaatg
    71101 agggctaatc cccgccgcca gcgcggcatt atccagacgt ttgctttcca tcgagcttcc
    71161 ttagcgtttt gcctgccaga tacgagcctg ataatcgcga atagagcgat ccgagctaaa
    71221 cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct ggtcgcggta
    71281 cagcacatcc acctgctttt gtgcctctac ataggctgcg aaatccgcca tcaccagata
    71341 cggatcgccg ccctgtttgc cgatactgtg cagcatctgg tcgaaggcat gcttatcgcc
    71401 gtcgctgtat ttaccgcttt ccagctcttt caatactgcg tccagcacct tatctttctt
    71461 ccgccatttc accgggtcgt agcctttggc cagaattgcc ttcacttgtt ccacggtatg
    71521 accaaaaata aagatatttt cttcaccgac tttctcggcg atttcaacgt tcgccccatc
    71581 cagcgtaccg acagtaagcg caccattgag cgccagtttc atattgccgg taccggaagc
    71641 ttctttacct gcagtcgaaa tttgttcgga gatatccgcc gccgggatca gtttttccgc
    71701 cgccgaaacg caataatccg gcaggaacac caccttcaac ttatcgccaa ccagcggatc
    71761 gttgttgatc acgtcagcca ctttgttgat cgcaaagata atatttttcg ccaggtagta
    71821 gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt acgcgatcag cctgtgggtt
    71881 ttcacgaatt tctttgtaca acgccagaat atgcagcaga ttcaggtgct ggcgtttgta
    71941 ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt ggattgatct caataccggt
    72001 acgaactttc acaaactccg ccagacggac tttattcgcc tgcttgatct cgcgatattg
    72061 ctgacggaat ttcgcatcat cagcgaattt ttccagattg atcagctgat cgagatcgtt
    72121 agcccactct tttttcagtg atttatccaa cagagccgcc agtgccgggt tgcactgttt
    72181 gatccagcga cgtggggtaa taccgttggt gacgttatgg aatttgttcg gccatagctg
    72241 gtgatattcc gggaacagat ctttcaccac cagatccgag tgcagcgccg caacaccgtt
    72301 caccgcgaaa ccgccaacca cacacaggtt cgccatatgc acttgtttgt cgtgcaccac
    72361 cgccagttta gcccacactt tttcatcgcc cggccaggtt ttctccacca gcgttttaaa
    72421 gcgagtatta atttcgttaa taatctgcat gtggcgcggc agtaagcctt tcaccagttt
    72481 cacatcccag cgttccagcg cttctggcat cagggtatgg ttggtgtaag cgaaagtttt
    72541 gctggtaatg gcccaggcgt catcccagct catctggtgc tcatcgatca gcacgcgcaa
    72601 cagttctgga atcgcgatag ttgggtgggt atcgttcagc tgaataactt cgtaatccgc
    72661 cagttcgtgc agtttacgcc ccgccagatg atggcgacgc aaaatatccg ctaccgaaca
    72721 ggcacactgg aagtactgct gcatcaagcg cagcttttta ccggcagtat ggttgtcgtt
    72781 tggatagaga actttggtca gtttttccgc attgatgccc tgctgttcgg cacgcaggaa
    72841 atcaccgtcg ttaaatttag tcagatcaaa cggatgcgcg tgcgtcgcct gccacagacg
    72901 cagcggctgc gccacgccat tacgatagcc gacaacgggg agatcccacg cttgaccggt
    72961 aatggtaaac tccggctccc agcgtccgtc tttcgtcact ttaccgccaa tccctacctg
    73021 cacatccagt gcttcgttgt ggcggaacca cgggtagtta ctgcgatgcc agtcatccgg
    73081 cgcttcaacc tgtttgccat ccacaaaaga ctggcggaac aaaccatatt gatagttcag
    73141 accgtaaccc gtcgcagact gaccgacagt tgccattgaa tcgaggaagc acgccgccag
    73201 acgtcccaga ccaccgttac ccagcgcagg gtcgatctct tcttccagca ggtcagtcag
    73261 attgatgtca taagccttca acgaatcctg tacatcctga taccagccga gattcaacag
    73321 gttgttgccc gtcagacgac caatcaaaaa ctccattgag atgtagttaa catgtcgctg
    73381 attcgccacc ggcttggcga atggctgagc acgcagcatt tcggccagtg cttcactcac
    73441 tgccagccac cactggcgag gagtcatttc agccgcagaa tttaagccat aacgctgcca
    73501 ctgacgtgaa agcgcttcct gaaattgctt atcgttaaaa ataggttgtg acataggagt
    73561 tccacttttc ttagattttc aacacaacgt tatcgctagt ttgccaggct cgatgttgac
    73621 cttcctcatc ctgcggggga ttaggcaggg aggagttgcg gggatgagca aggaaatgtg
    73681 atctcgacca cttaaagcta gtgcaaacca caggattagc agcaaatcaa tgccatacag
    73741 cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa aaagcgaaag
    73801 gtaactctat aaattaagta aaggagtgaa acagtttcac aagtaaaata tccagtgtgc
    73861 tccatctcat tcttaataga tttattaaga tcatcttttt agatagcact ttcatcagga
    73921 atgaagaaga aacccttgct taaatgaatc tgatgaacat aagggaaacc agtattcacg
    73981 ctggatcagc gtcgttttag gtgagttgtt aataaagatt tggaattgtg acacagtgca
    74041 aattcagaca cataaaaaaa cgtcatcgct tgcattagaa aggtttctgg ccgaccttat
    74101 aaccattaat tacgaagcgc aaaaaaaata atatttcctc attttccaca gtgaagtgat
    74161 taactatgct gattccgtca aaattaagtc gtccggttcg actcgaccat accgtggttc
    74221 gtgagcgcct gctggctaaa ctttccggcg cgaacaactt ccggctggcg ctgatcacaa
    74281 gtcctgcggg ctacggtaag accacgctca tttcccagtg ggcggcaggc aaaaacgata
    74341 tcggctggta ctcgctggac gaaggtgata accagcaaga gcgtttcgcc agctatctca
    74401 ttgccgccgt gcaacaggca accaacggtc actgtgcgat atgtgagacg atggcgcaaa
    74461 aacggcaata tgccagcctg acgtcactct tcgcccagct tttcattgag ctggcggaat
    74521 ggcatagccc actttatctg gtcatcgatg actatcatct gatcactaat ccagtgatcc
    74581 acgagtcaat gcgcttcttt attcgccatc aaccagaaaa tctcaccctg gtggtgttgt
    74641 cacgtaacct tccgcaactg ggcattgcca atctgcgtgt tcgtgatcaa ctgctggaaa
    74701 ttggcagtca gcaactggca tttacccatc aggaagcgaa gcagtttttt gattgccgtc
    74761 tgtcatcgcc gattgaagcc gcagaaagca gtcggatttg cgatgacgtt tccggttggg
    74821 cgacggcact acagctaatc gccctctccg cccggcagaa tacccactca gcccataagt
    74881 cggcacgccg cctggcggga atcaatgcca gccatctttc ggattatctg gtcgatgagg
    74941 ttttggataa cgtcgatctc gcaacgcgcc attttctgtt gaaaagcgcc attttgcgct
    75001 caatgaacga tgcactcatc acccgtgtga ccggcgaaga aaacgggcaa atgcgcctcg
    75061 aagagattga gcgtcagggg ctgtttttac agcggatgga tgataccggc gagtggttct
    75121 gctatcaccc gctgtttggt aacttcctgc gccagcgctg ccagtgggaa ctggcggcgg
    75181 agctgccgga aatccaccgt gccgccgcag aaagctggat ggcccaggga ttccccagcg
    75241 aagcgattca tcatgcgctg gcggcaggcg atgcgctgat gctgcgcgat attctgctta
    75301 atcacgcctg gagtttgttc aaccatagcg aactgtcgct gctggaagag tcgcttaagg
    75361 ccctgccgtg ggacagtttg ctggaaaatc cgcagttggt gttattgcag gcgtggctga
    75421 tgcaaagcca acatcgctac ggcgaagtta acaccctgct ggcccgtgct gaacatgaaa
    75481 tcaaggacat cagagaaggc accatgcacg cagaatttaa cgctctgcgc gcccaggtgg
    75541 cgattaacga tggtaatccg gatgaagcgg aacggctggc aaaactggca ctggaagagc
    75601 taccgccggg ctggttctat agccgcattg tggcaacctc ggtgctgggt gaagtgctgc
    75661 actgcaaagg cgaattgacc cgctcactgg cgctaatgca gcaaaccgaa cagatggcac
    75721 gccagcacga cgtctggcac tacgccttat ggagtttaat tcagcaaagt gaaattctgt
    75781 ttgcccaagg gttcctgcaa accgcgtggg aaacgcagga aaaagcgttc cagctgatca
    75841 acgagcagca tctggaacag ttgccgatgc atgagtttct ggtgcgcatc cgagcacaac
    75901 tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc ggcgcgtagc gggattgaag
    75961 tcttatcgtc ttatcagcca cagcaacagc ttcagtgcct ggcaatgttg attcaatgct
    76021 cgctggcccg tggtgattta gataacgccc gtagccagct gaaccgtctg gaaaacctgc
    76081 tggggaatgg caaatatcac agcgactgga tctctaacgc caacaaagtc cgggtgattt
    76141 actggcaaat gaccggcgat aaagccgccg ctgccaactg gttgcgtcat acggctaaac
    76201 cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg caacattgcc cgcgcacaaa
    76261 tcttgctggg cgagtttgaa cctgcggaaa ttgtcctcga agaactcaat gaaaatgccc
    76321 gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt gctgctaaat caactgtact
    76381 ggcaggccgg acgtaaaagt gacgcccagc gcgtgttgct ggacgcatta aaactggcga
    76441 atcgcaccgg atttatcagt cattttgtca tcgaaggcga agcgatggcg caacaactgc
    76501 gccagctgat tcaactcaat acgctgccgg aactggaaca gcatcgcgca cagcgtattc
    76561 tacgagaaat caatcaacat catcggcata aattcgccca tttcgatgag aatttcgttg
    76621 aacgtctgct aaatcatccg gaagtgccag aactgatccg caccagcccg ttgacccaac
    76681 gtgaatggca ggtcctgggg ctgatttact ccggttacag caacgaacaa atagcgggtg
    76741 aactggaagt ggcggcaacc accatcaaga cgcatatccg caatctgtac caaaaactcg
    76801 gcgtcgccca ccgtcaggct gccgtgcagc acgcgcagaa actgctgaag atgatgggtt
    76861 atggggtgtg agtttgtata gcgtttttat gtacactctt tatactgtat ataaaatcgc
    76921 actacaggct catgaatatg aaaccggaaa gcaaagaagc gccaatcaat atcagagcga
    76981 aagcctcaca gcgtgacctg attgatatgg cagcgaatct ggtcgcaaaa tcgcgcactg
    77041 attttatgct ggatgccgca tgccgtgaag cgcaagacat cttattagat caacgactat
    77101 tcatccttga tgacgagcaa tacgatgcgt ttctcgctgc cctggacgcc cccatcactg
    77161 ctgagcgtca ggccaaaatt aacgcgttga tgaatcgtaa atcgccatgg gaataacggc
    77221 tcctactccc ctaacgtctg aacataatct ggctgacttt tgctgttccg atcacgggat
    77281 gaatgaatgg ctaaaaaaga aagcgttaaa aaatcacagc tccggtctgt ctcgtgttta
    77341 cgttatctgt attgccaata cccgccaggt aatcggctat tactgccttt ctacaggcag
    77401 tattcaacgt aatttggccc ctggagccat gcgacgtaat gctcccgaat cgttgcctgt
    77461 tgtcgtatta ggcagactgg caattgatca agcctgggca ggtaaagggt tgggcgtcgc
    77521 attgttgaaa gatgcagtct atcgcacaat gtccatagct caacaggtag gggttcgtgc
    77581 gctcattgtt catgccttag acgattcagt acgcaatttc tatctgaagt atgcctttgt
    77641 accttcacca ttccagtcac ttaccttact gtatcccatt actctggagt aatttacctc
    77701 taccggatag tcacactgat gagtctgatc cgggagggat ttcaatcagt gagtttggtt
    77761 atcatcaccc gcgttacgcc atctgtttca gccagagtaa accgcaccgg caagaaacgc
    77821 tccaccacgg cgatattggt cagcagatgg catgagggat gggcgaccgt aaattgtccc
    77881 gcgcccgcca gcgccattgg caacactagc tggtcagcaa gatattcccc cactgccgcc
    77941 gggcttgcca ggtagcgttt cacctctttc accaactgtg ccgcaaccac ctcggcactg
    78001 acgcgctttt caccgacgac aaaaaagcgt tcggtgatat tttcactttc gacttcaagc
    78061 gagacggtat tacccggccc ctagtcgcgc ggcaggttat gaatattctg ctcatgcagg
    78121 gaaaaacttg ccgccagcgt agcgatttca cgctcagcga catgccgcgg cacgccagct
    78181 aataacacct ctccacgcat ctgcacaatg ttcccgcgct cgccaagttg caaggtgtta
    78241 aacgatgcca ccggcgagac ttccgttgcc accacgccgc ctccggcagg ataaaaaccg
    78301 tgacgtaaca gcgtggtttg ctgatgaatt cctattttcg ccagcagcgg ctccaacacc
    78361 cggcggataa aatccgcagg cggggccgac gggttatcgg taccgccgct cacttcaaca
    78421 cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg tttgcagcac cagcgtacaa
    78481 cttcctgcgc taccgatagc aaagcgataa tcgccgccac gcacggtgcc gggccggaag
    78541 accagacgct gcgaccccag ctccgcgcct tccaccgtcg ccccgcaaat ttccgtcgcc
    78601 gcctttaccg ccgtcagatg ctggcgcaac agtcccggtt ttgcccgccc ggcacgaatg
    78661 ccggtgatgg taaatggctg gccggttatc atcgacagac tcagcgccga gcgcatgatc
    78721 tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat catttatcct
    78781 tttacgcaca ccacctgacg tagggtgtag ataacttcca ccagatcgct ttgtgccgcc
    78841 atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc ggcatcttta
    78901 cggcattcca catgcacggt ggcacgaatt tgatcttcca cgctgaacat ttttttcgct
    78961 ttagtacggc tcataacccg cccggcaccg tggctgcacg aacagaacga ctcttcattt
    79021 cccagcccac ggacgataaa gcttttcgct cccatcgacc ctggaataat tccatattga
    79081 ccagcacgcg cagacaccgc gcctttacgc gtcacgtaga tctcttcacc aaagtgctgt
    79141 tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt ttgtggctgt
    79201 ctgaccgttt tctgcgtaat gctctgcaac gccgttacta cgttttccat catcacatcg
    79261 cggttaaggc tggcaaaaag ctgcgcccag gccacggctt tcaggtaatc atcaaagtat
    79321 tccgtacctt ccataaagta cgccagatca cgcgacggca atgtttcaag cgtttcctgc
    79381 atctcttttt gcgccagatc gataaagtaa gtcccgatgg cattaccaat tccgcgtgaa
    79441 ccggagtgca gcataatcca cacctggtcc gactcatcaa ggcagatttc aataaagtgg
    79501 ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa acgcggatat
    79561 ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc gacgttaaca
    79621 ggcgggtttt cccatgcgcc tttatcacgt ttacaacggc cagtggtacg cccgtgcggc
    79681 accgcagttt caatcgcctg acgcagctcc gccaggtttt ccggcaggtc agccgccgtt
    79741 aaagcggtac gtagtgcgtt cattccacag ccaatatcca cgcccaccgc cgccggaata
    79801 atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag gtgtacatca
    79861 ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat aagttgctga
    79921 cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc attttcagtg
    79981 gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc ctgataattg
    80041 caaccgtcgt gccagaaaat taaataagca gattaatttt ttaattcatt gttttaaaaa
    80101 gattatatct ttacgtccgt aaacggagat ttcccgcaaa gccaatttac cgataatgaa
    80161 atatcgtctt ttataaggat atctaagatg cgtaaaacag tggcttttgg ctttgtcgga
    80221 acagtgctgg attatgccgg gcgcggcagt cagcgctggt cgaaatggcg tccgtcactc
    80281 tgtatatgcc agcaagaatc gttagtcatc gatcgactgg aattattgca tgacgcccgc
    80341 tcgcgctcgc tatttgaaac gcttaaacgc gatatcgcca gcgtttcgcc agaaaccgaa
    80401 gtggtgagcg ttgagattga actgcataac ccgtgggatt tcgaagaggt ttacgcctgc
    80461 ctgcatgatt tcgcccgtgg ttacgagttt cagccagaaa aagaagacta tttaattcac
    80521 atcaccaccg gcacccacgt cgcgcagatt tgctggtttc tgctggctga agcgcgttac
    80581 ctgcccgccc ggttgataca atcttcacct ccgcgcaaaa aagaacagcc gcgcggcgca
    80641 ggtgaagtga cgattatcga tctcgattta agcctttata acgccatcgc cagccgcttt
    80701 gccgaggaac gccagcaaac gcttgatttt cttaagtccg gtattgccac gcgtaacccc
    80761 cacttcaacc gcatgattga gcagattgaa aaagtagcga ttaaatcccg cgcaccgatc
    80821 ctgctaaacg gcccaaccgg tgcgggcaag tcatttctgg cgcgacgtat atttgaatta
    80881 aaacaggcgc ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc aaccctgcgc
    80941 ggcgataccg ccatgtcgac gctgtttggt catgtgaaag gcgcgtttac cggggcacgg
    81001 gaatctcgtg aaggattatt acgcagcgcc aacggcggaa tgttgtttct tgatgagatt
    81061 ggcgaactgg gcgcagacga acaggcaatg ctgctgaaag ccattgaaga aaaaaccttt
    81121 tatccgtttg gcagcgatcg ccaggtgagc agtgattttc agcttatcgc cggaacggtg
    81181 cgtgatttgc gccagctggt tgccgatggc aaattccgcg aagatctata cgcgcggatc
    81241 aatctctgga ccttcaccct gccggggcta cgccagcgtc aggaagatat tgaacctaac
    81301 ctggattatg aagtggagcg ccacgcttca ctcaccggcg acagcgtgcg ttttaacacc
    81361 gaagcgcggc gcgcctggct ggcctttgcg acatctcccc aggcaacatg gcgcggtaac
    81421 tttcgcgagc tttctgccag cgtcacgcga atggcaacct ttgcctctag cggacgcatt
    81481 actctggacg tggttgaaga tgagataaac cgtctgcgct ataactggca ggagagccgc
    81541 ccctccgcgc ttacggcgtt gctgggcgca gaggcagaaa acatcgatct cttcgaccgt
    81601 atgcaactgg aacacgttat cgcaatctgc cgccaggcaa agtcgctttc cgcagccgga
    81661 cgccagcttt ttgacgtttc gcgccagggc aaagccagcg tcaatgacgc ggatcggcta
    81721 cgcaaatacc tggcgcgttt tggtctgacg tgggaagccg tgcaggatca gcacagctcc
    81781 agttgaatat ggtggtccgt cagcacctgc atcacgctta ctggcggcgg ggtgtcggtg
    81841 tagacggcat ctaccatgct gatgctaccc atattgacca tcgcgttacg gccaaatttc
    81901 gagtgatcga caaccagcat aacgtggcgc gagttctcaa taatggcgcg tttggtgcga
    81961 acttcgtggt aatcgaactc cagcagcgag ccgtcgctat cgatgccgct tatccccaga
    82021 atgccgaaat caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc aatgatcccg
    82081 ccatcgcggc tgcgtaattc gccaccggcg agaataatac ggaaatcctc ttttaccatc
    82141 agcgtgttag caacgttaag attgttggtg acaatgcgca aattgctgtg attgagcagc
    82201 gcgtgcgcca ccgcttccgg cgtggtgccg atatcgataa acagcgtcga gccattgggg
    82261 atttgctccg ccactttgcg ggcgatgcgc tctttttctt cggtctgggt ggctttacga
    82321 tcgtgccacg gcgtgttaac cgaactggaa ggcagcgccg caccgccatg atggcgcagg
    82381 atcaggtttt gctccgccag ctcattgagg tcgcggcgaa tagtctgcgg gctgacggag
    82441 aagtgctcca ccagctcctc ggtactgaca taaccctgct gtttaaccag ttcgataata
    82501 ccgttgtgac gttgtgtttg tttcatttct aaatccctgg aattattttc gttttcgcgc
    82561 attgagcgaa tcaacaaaag ccatcgctaa acccacggct aacccggcga tgtgtgctcc
    82621 gttcgccatc gacatcccaa acaaatcaaa ccatccggcg acaatccaga tcagcgcaaa
    82681 gataattaac ccacgttgca ggtaaatgcc actttgcgga tcgcgttcgc cacgtagcca
    82741 gacgtagccc atcagcgcat acaccacgcc agaaagcccg ccaaaccacg gcccgctgaa
    82801 tttttgctgc acatagccgc ttaacagggc gctgataagc gtaatgacaa ttagcttacc
    82861 gctaccgagg cgtttttcca ccgcaccgcc gagataccac caccagagca ggttaaagag
    82921 gatatgcatc agcgagaagt gcattaacgc gtgggtgaag taacgccaga actcaaattt
    82981 cagtgctgga tcgaatggcc aagccagcca taacatcact tcctgatcgc cgagaatttg
    83041 catggcaata aacaccacca cgcaggcgat catcatcacc caggtcaccg gacctgcgcg
    83101 ttcacgcaag gcggcaaaga aaggataacg gcgataatgc aggccactgc cggtatggcc
    83161 tgcctgccag ctcgccgcca gataacgcgg atctgccggg ttttcgagaa aacgcgccag
    83221 ctccgcccgt acgcgctcgg cctgggactc atccgccagc cagacatcgc tttggttatg
    83281 ttgttgaatc gtgaggataa caccctgcgt cgccatgtaa tcaacaaacg cctgcgccac
    83341 gcgggggtta gcaaaagagg taatcatcaa catcgttgct gtcgcttatt ccacacaaaa
    83401 ggggacagta taaagcgtta cgcgccgtac gccacctctg cgggaaactg acgttgccag
    83461 gcttcaaagc cgccgtcaat gctatagacc acatcgtagc cctgttgcag cagatactgc
    83521 gccgcgcctt tgctgctatt gccgtgataa cacatcacca tcaccggagt gtcaaagtcg
    83581 ttattacgca taaaagcgcc cagcgtgtcg ttggttaaat ggaaagcctg caccgcatgt
    83641 cccattgcga aactctgtgg atcgcgaata tcgaccagca ccgcctcttt ttcctgcaac
    83701 ttctggtgcg cgtcggcaac gttaatacat tcgaactgat ccatgcgtct ctctttcttt
    83761 acaaacaagt gggcaaattt accgcacagt ttacgtcgaa gcggcagata aacgccataa
    83821 tgttatacat atcactctaa aatgtttttt caatgttacc taaagcgcga ttctttgcta
    83881 atatgttcga taacgaacat ttatgaactt taacgaaagt gaatgagggc agcatggaaa
    83941 ccaaagatct gattgtgata ggtggcggca tcaatggtgc tggtatcgcg gcagacgccg
    84001 ctggacgcgg tttatccgtg ctgatgctgg aggcgcagga tctcgcttgc gcgacctctt
    84061 ccgccagttc aaaactcatt cacggtggcc tgcgctacct tgagcactat gaattccgcc
    84121 tggtcagcga ggcgctggct gaacgtgaag tgctgctgaa aatggccccg catatcgcct
    84181 tcccgatgcg ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg atgattcgca
    84241 ttggtctgtt tatgtacgat catctgggta aacgcaccag cctgccggga tcaactggtt
    84301 tgcgttttgg cgcaaattca gtgttaaaac cggaaattaa gcgcggattc gaatattccg
    84361 actgttgggt agacgatgcc cgtctggtgc tcgccaacgc ccagatggtg gtgcgtaaag
    84421 gcggcgaagt gcttactcgt actcgtgcta cttctgctcg tcgcgaaaac ggcctgtgga
    84481 ttgtggaagc ggaagatatc gataccggca aaaaatatag ctggcaagcg cgcggcctgg
    84541 ttaacgccac cggcccgtgg gtgaaacagt tcttcgacga cgggatgcat ctgccttcgc
    84601 cttatggcat tcgcctgatc aaaggcagcc atattgtggt gccgcgcgtg catacccaaa
    84661 agcaggccta cattctgcaa aacgaagata aacgtattgt gttcgtgatc ccgtggatgg
    84721 acgagttttc catcatcggc accaccgatg tggaatacaa aggcgatccg aaagcagtga
    84781 agattgaaga gagtgaaatc aattacctgc tgaaagtgta taacgcgcac tttaaaaaac
    84841 agcttagccg tgacgatatc gtctggacct actccggtgt gcgtccgctg tgtgatgatg
    84901 agtccgactc gccgcaggct attacccgtg attacaccct tgatattcat gatgaaaatg
    84961 gcaaagcacc gctgctgtcg gtattcggcg gtaagctgac cacctaccga aaactggcgg
    85021 aacatgcgct ggaaaaacta acgccgtatt atcagggtat tggcccggca tggacgaaag
    85081 agagcgtgct accgggtggc gccatcgagg gcgaccgcga cgattatgcc gctcgcttac
    85141 gccgccgtta tccgttcctg accgaatcgc tggcacgtca ttacgcgcgt acttatggca
    85201 gcaacagcga gctgctgctc ggcaatgcgg gagcggtaag cgatcttggg gaagatttcg
    85261 gtcatgagtt ctacgaaacg gagctgaaat atctggtcga ccacgagtgg gtccgccgcg
    85321 ccgacgatgc cctgtggcgt cgcacaaaac aaggcatgtg gctgaatgcg gatcaacaat
    85381 ctcgtgtgag tcagtggctg gtggaatata cgcagcagaa gttgtcgctg gcgtcgtaaa
    85441 ttaacgtaag gtgatcaggt cagatttcaa tctggcctga gactgatgac aaacacaaaa
    85501 ttgcctgatg cgctacgctt atcaggccta cgtggtttat gcaatatgtt gaatttgcat
    85561 gattttgtag gcctgataag gcgttcacgc cgcatcaggc atgaacaaag cgccacgttg
    85621 tcaacaatct gacctacttt ttgattcagg gaattaccgc aaagcccacg ccatcatacc
    85681 caacgtaaca aaacttaagc acgttcccca ctcactccac ttccgtatct ggcttctatt
    85741 tttgctacgc cactggcaat aatatcccaa cgcaaaacca agctgtgcac ttatcggacc
    85801 gacaaagtcg atccactgtt gagaccaaaa tggcgaaaaa ttcagtggga gccagacaac
    85861 tgtcagaaag aaactgcgca tattttccag caaagcaatt tgcgctggcg cgacataaaa
    85921 aatgatattc cacaggagca accagaaata aactggtgca aataccagcc aacaatgcag
    85981 tctgcttttc aattcaggtg gcgttctgcg cgcaaattgc caaccaacgc ccaccattgc
    86041 acagaagaaa agcgcatatt cagccgataa ccatagttgt aattgcagaa gggaaatacg
    86101 cctgtgcatg agcgggattg agaacagtat ttctcccaga aaactcagcg tgattacaca
    86161 ggcagccagc gcaaccaccg tcggccacac caacaacggt acaataacaa agccgagaaa
    86221 taacggataa atataaagtc tgattatatt catcattatt gtcctgatga ttcgggggat
    86281 gcttcataca gcggcacata ccccacatcc tctaccaact gttgcccctg ctgacttata
    86341 aaccagtcaa caaatttctg cgtttccggc gtgggatttt cacgcgtcac catataggca
    86401 tccacaatgt atggataagt gccgttgcgg atattttcct cggtaggcgc aacgccattt
    86461 atagccaaaa gtttaatctg cttgcgcatt ttgagcatat tgtcattcat acgagtgacg
    86521 taatagtgga aggtgtaccc aatggaagat ggcgtgtttt gatagtcggc aacttcagta
    86581 attaaaccgc ccataagatc aatcacagtg gattttttag ctggcagcat tggggtgtct
    86641 tgcataacca accccttcat aatcgtttgg cttccagaat cttcagggcg ctgccagact
    86701 tttatatgtt cgtcactacc acctacttta ttccagcggc tagttttgcc gctaaaaata
    86761 tctttaacct ggtgctcaga gagagaattt accgggttat taatatcaac gataaagaca
    86821 aaggcttcgc gggcgaaagc ggtatatttc agtttaacac ccgcttcttc tgcacgtttt
    86881 ttctgtcctt ccgacggttg tgcaacaaaa ataaccgtcg cggaaccttt aatcagacta
    86941 tcgtaggctt cagctgttcg ggtttgctgc aaaccatacg cctgccaggc cagcgaggtt
    87001 cgatccttat cttttggctc gggtactaat ttataatacg cggaggcata cagcgggtat
    87061 aaggctgtag cgccgtacac tctcggccat cttgaccaat catcgctgta acgtaatgca
    87121 ggttgccccc gcagagacgt cagtccatgg ctctcttctt ccctgacgct gttatagtac
    87181 ccttcccggt cgacacatcc cgccagtaac gccgtgccta atagtaaaac accggtgaat
    87241 ttattcatct cgctgattcc tttctctcga gcgttattcc attgttagat acagcggcac
    87301 atatcccaca tcttctacca gactctgccc ctgcggcgtt aaaaaccatt cgaccagttt
    87361 ttgtgtttct gacgttgtat tttctctcgt caccataaat gcatcgacga tgtacgcata
    87421 tttgccgttg cgaatgtttt ccgccgtcgg tgtaataccg ttaatcgcta gcaatcttat
    87481 atttttatca gcattcattt gcgtcgcgta atagcggaag gtatagccta ttgcgttgtt
    87541 tgtattacgg tattcggcaa cgactttaat cattccctcc atcacgcttg ccacttccgt
    87601 ttcctgcggc gagatcatgc ggactttttt catgacctgt gattgcatca ctgtctggct
    87661 gccagagtct tccgggcgct gccaggtctg gatctcctga tcgttaccgc caacagtacg
    87721 ccaattggta attgcaccac tgaaaatgtc acgcacctgt tgttcagtca gggaattaac
    87781 cggattatcc gcattgacga tgaaaacaaa tgcttcacgg gcaaatggcg tgtatagcaa
    87841 agtgacgccc gattcctcag cgcgtttttt ctgcccgccg gagggttgcg ccacaaaaat
    87901 aatatcggca tcacctttaa caatccggtt atatgcatcg ggggtacgag agttctccag
    87961 atattcccaa acgtgaaaat cctctggtat tacacttaat gcataaaatg cagaagcata
    88021 aattgggtac gcagccgtgg cgccatcgat tcgcggccag ttttgcctaa actgaatttg
    88081 cggtttgcca cgcagcggcg tcagtcgatt attgagttta tcgggtcgcc aggccctgat
    88141 atcaatattt tcgcgtacac cgacaccagg attttgctgc ttatataaat gggcctgata
    88201 cccggcgaca ataccaagta agagaagtag aaacaggagt aagttacggc aacgtagtcc
    88261 ggacgtgccg gataaaggat atctggtcgg ccaggcgtat cccagcgaaa aactgatatg
    88321 cgccccaacg ggaataatga ccatccacca gtactcacca cccactaatg agactaaaaa
    88381 aacgcccaac cagggaagca taaggtttgc caaccaacca gctcccatct ggcgaaattg
    88441 ataatctgcc agatgtagcg tgattatcca gccaaccagg gttaatagcg cgggtaagag
    88501 aaaaaagcat tgccgccaga cggctatttc tctgttttct ggcgcgctat caccggccca
    88561 cacgcgacca gagaaaaata ttattatcgc ccccagcgtc agcataaaga cgttgagtag
    88621 aagataaatt gttggcgtga taaggatatt acaaacttca ataataccgg taagcccgac
    88681 acccagcatg gcaacaaccg ctgccaaaaa ttgcgccagt atggggatgc cgaaaaaagt
    88741 cattatcagc gaggtcaaaa tccattttct atttggcatc attctgtcca ttcttcctga
    88801 atgacggaat tatactcccc gagaaccctc gccccctctg gacacttttc cgaaataaag
    88861 gcgaaaaaaa cgggaccttg tggccccgtt ctgtttttta gcgaacttac aatctcaccg
    88921 gatcgatatg ccagatatga tcagcgtact ctttgatagt acggtcagaa gagaagtagc
    88981 ccatattggc aatgttcagc atcgcttttg cggtccactc ttcctgaagc tcgtagagtt
    89041 catcgacttt atcctgacaa tcgacatagc tgcgataatc cgccagtacc tggtagtgat
    89101 cgccgaagtt gatcagcgaa tcaaccagat cgcgatagcg acccggatct tccggactga
    89161 atacaccgct gccgatttgc gtcagcacct gatgcagctc ctcatctttc tcgtagtatt
    89221 cacgcggttt gtagccctga cgacgcagtt cttccacttc ttccgctgtg ttaccaaaaa
    89281 taaagatatt gtcagcaccg acatgatcca gcatctcgac attcgcaccg tccaacgtac
    89341 cgatagtcag cgcaccgtta agcgcaaact tcatgttact agtgccggaa gcttccgtcc
    89401 ctgccagcga aatctgttca gacagatctg ccgccggaat gatcaactgc gccaggctaa
    89461 cgctgtagtt cgggatgaac acgactttca gcttatcgcc aatctgcgga tcgttgttga
    89521 tcactttcgc tacgtcattg atcaaatgga taatatgctt cgccatgtaa taggccgaag
    89581 ccgccttacc gccaaaaata ttcacgcgcg gtacccactt cgcatccagg tcggccttga
    89641 tgcggttata gcgggtaatc acatgcaaca cattcatcaa ttgacgtttg tattcgtgaa
    89701 tacgtttgat ttgcacatcg aacaacgcct ttggattcac caccacattc agctgctggg
    89761 cgatatactc cgccagacgc tttttattct ccagcttcgc ctgatgcaca gcgtgattaa
    89821 ccattgggaa atcacagtgt tgttgcagct cattaagcag gctaaggtcg gtgcgccagt
    89881 tacggcccag gtgttcgtcc agcacggctg aaagcgatgg gttcgctacc gccagccagc
    89941 gacgcggcgt cacaccgttg gtgacgttgg tgaaacgacc cgggaagatt ttcgcaaagt
    90001 cggcaaacaa cgattgcacc atcagattag agtgcagttc cgatacaccg ttaactttgt
    90061 ggctcacaac aaccgccagc caggccatac gcacacgacg accgttggat tcatcaatga
    90121 tcgacgcccg tcccagcaga tcggtatcgt tcggatactg ttcctgcaag gttttcagga
    90181 aatagtcgtt gatttcaaag atgatctgca ggtgacgcgg cagaatttta cccagcatat
    90241 caaccggcca ggtttccagc gcctcgctca tcagcgtgtg gttagtgtag gagaagacct
    90301 gacaacacac ctcaaacgcg tcgtcccagc taaattggtg ctcatcgatc agcagacgca
    90361 tcatctcagg aatcgacagt accggatggg tatcattgag atgaatcgcg attttatccg
    90421 ccaggttatc gtaggtttta tgcaactgat aatggcggct taaaatgtcc tgaatggtcg
    90481 aggaaaccag gaagtattcc tgacgcaggc gcagctcacg cccggagtag gtggagtcat
    90541 ccggatacag tacgcgagat acgttctcgg agtggttttt atcttccact gccgcgaagt
    90601 agtcaccctg gttgaattta ccgaggttaa tttcgctact ggcttgcgca ctccacaaac
    90661 gcagcgtgtt ggtcgcgtcg gtgtcgtaac cagggattat ctgatcgtaa gcgactccca
    90721 gaatctcttc ggtttcaatc cagcgcgttt ttttaccttc ctgctgaatg cgaccaccaa
    90781 aacggacttt atagcgcgtg ttgtggcgtt tgaattccca cgggttacca tattccagcc
    90841 agtagtcagg cgactctttc tggctaccgt taacgatgtt ctgcttgaac ataccgtagt
    90901 catagcggat gccgtaaccg cgccccggca accctaacgt cgccagagaa tcgaggaagc
    90961 aagccgccag acgtcccagg ccaccgttac cgaggcccgg gtcattttct tcatcaatca
    91021 gctcttcgag atttaacccc atcgcttcca gtgcgccctg tacatcttcg taaattccta
    91081 gcgacaacat ggcgttggag agcgtacggc caatcaaaaa ctccatcgac aggtagtaaa
    91141 cctgacgagt ttcttgcgac aactgggcac ggtttgaacg taaccagcgc tccacgagac
    91201 gatcgcgcac agcaaataac gttgcgttca gccattcatg tttattggcg acgaccgggt
    91261 cctttccaat cgtaaacatc agcttgtaag cgatagagtg cttaagagct tctacgctaa
    91321 gcgtgggcga tgaatatgta aacggagcat tcatataggc gtttcctgaa aactatttca
    91381 agcgatagta aagctcacgg tacgacttcg ccgcgacctg ccagctaaaa tccattgcca
    91441 tagcctgacg ttgcacaaac cgccacagag aaggacggga ccacagtaca aaagcacgtc
    91501 gaatagcccg taacagcgac caggcattac tatcttcaaa gacaaaccca ctggcgacgc
    91561 catctgcaag gttctcaaga gaacagtcag aaaccgtatc agcaagccca ccggtgcgcc
    91621 gcactaacgg cagcgtaccg tacttcaatc cataaagttg cgttaagccg cacggttcaa
    91681 aacggctggg caccagaatg acgtccgcac cgcccataat gcgatgcgaa aatgcttcgt
    91741 gatagccaat ctgaacgccc acctggccgg ggtattccgc tgccgccgca aggaaacctt
    91801 cctgcagcac cggatcgccc gcgccgagta gcgccagctg cccgccctgc tccagaagac
    91861 ccggtaaggc ctccagcacc aggtcgaggc ctttctggct ggtcagacgg ctcaccactg
    91921 caaaaagcgg cactttatcg tcaaccttaa gccccattgc gatttgtaac tggcgcttat
    91981 tttccgcttt atcttccaac gtatcgcggg tgtaacgcga ggccaatagt aagtccgtct
    92041 ctggactcca gattttctcg tccacgccgt tcagtacgcc ggaaagacgc ccttcacgat
    92101 gacgctgttg caacaaacct tccataccgt aggcaaactg cggttcggtg atctcgcgag
    92161 cgtaggttgg actgaccgcc gtaatgtgat cggcatagta cagaccggcc ttcaggaaag
    92221 agatttgtcc gttgaattcc agcccatgaa tattaaagaa tgaccatggc aattggatgt
    92281 cattcatgtg atgtgcataa aacatgcctt gataggccag gttgtgcaca gtaaacaccg
    92341 acttcgccgg acgcccgcgc gccgccagat acgcaggcgc aaggcctgca tgccagtcgt
    92401 gcgcatgcac cacatcagga cgccagaatg ggtcaagccc gctggccatt tctgccccaa
    92461 cccaccccag cagcgcaaaa cgcaatacgt tgtcggtata ggcaaataag ttggtatcgt
    92521 gatacgggct tcccggacga tcatagagat gcggcgcgtc aatcaggtat atgccaaccc
    92581 cgttgtaatg accgaacaac agcgtgatat gcccggcgaa ggtatcacga cgggatacta
    92641 cctgcgcatc ggtcacgcca cggcgaatat cgggaaatgc aggcaacagt acgcgagcgt
    92701 caacgccgtc tgcgatttgt gctgcgggta atgccccaat aacatcagcc agaccgccgg
    92761 ttttaagcag cgggaacatc tctgaacata catgtaaaac ctgcattatc gctcctgttt
    92821 atgccctaac ttccgtagca tttcgcgcgt taccagcacg atgccttctt ctgaacgata
    92881 gaaacgacgt gcatcttcct ctgcgttttc accaatcacc atgccttccg gaataacaca
    92941 agcacgatcg atgacgcagc ggcgcagacg gcacgagcga cctacccata cttccggtaa
    93001 caatacggcg gaatcaatgt tgcagaatga attcacgcga acgcgcgaga acagaacgga
    93061 ctgcaccacc accgaaccgg agatcacaca accgccggaa accagtgagt taagggtcat
    93121 cccgtggcta ccggagcgat cctgcacgaa tttcgctggc ggtaatgatt cattgtaggt
    93181 gcgaattggc caattgcgat cgtacatatc cagttccggc accacagagg ccagatcgag
    93241 gttcgctttc cagtaagctt ccagcgtacc cacatcgcgc cagtacggct cggcatccgg
    93301 atcggattgt acgcaagaga gcgggaacgg gtgcgcatag gccagaccgg cttcggtgat
    93361 cttgggaatc aaatctttgc caaagtcgtg gctggagttc tcatcgcgat cgtcttcttc
    93421 cagcagttca tacagatagt cggcgtcaaa gacgtagata cccatactcg ccagagattt
    93481 gctcggatcg ttcggcattg acggcgggtt agcaggtttt tcaacgaatt cgataatttt
    93541 atcgttctca tcaaccgcca taacgccaaa tgcggaggct tcttcaatcg gtactggcat
    93601 acaagcaacg gtgcaacgtg cgcctttttc gacgtgatcg ataagcatac gcgagtagtc
    93661 ttgcttgtag atatggtcgc ccgccaggat caccacgtat tccgctttat aacggcgaat
    93721 aatatcgagg ttttgggtga ccgcatctgc ggtgccgcga taccagtttt cccctttcat
    93781 tctctgctgt gctggcagca gatcgacaaa ctcgttcatt tcttcattga agaatgacca
    93841 gccgcgctga atgtgctgca ccagagtgtg ggactggtac tgggtgatca cgcccatacg
    93901 acggatcccg gagttgatgc agttagacag cgcaaagtcg ataatgcgga acttaccgcc
    93961 gaagtgtacg gccggttttg ctcgcttatt ggttaaatcc ttcaggcggg taccacgtcc
    94021 tcccgccagt atcagggcaa cagatttcaa tggcagctgg cgcgccaaca ttaagtgatc
    94081 gttcttctct aaactaacca tgactaactc cttttttatc atctctggaa cacacacaat
    94141 ccgtgtgcag gtccctgcca gacagccgta atcactgggt tatcctctcc agcgaatggg
    94201 ggaatggcgt gccactcccc agcaggtaaa acaatctctg ttacctcaag cgtggcgtta
    94261 attgcgatca aaaagcgatc cgagagcaga atttgcagct gtttcggccc gttttgccac
    94321 tcatccgtgc ttaaaggttg agcatatcga tttagccaac ggacattgcc gtcgccttct
    94381 tcccaccagc gattctccac caaagcggga atacgcttgc gcagatggat taacgcgacg
    94441 gtaaatgcgg ttaaaccact gcttgcctgc gaccagtcca accaggttaa ttggttatcc
    94501 tgacagtagg cattgttatt gccatgctgg ctgtgaccat gttcgtcacc ggccagtaac
    94561 atcggcgtac cctgggagag caacaacgtt gttaacaggg cgtgaatgct gtcgcgccgc
    94621 cgttcaacca ggtcaagggt accgcctacc ccttctttac catgattgtt actgtaattg
    94681 ttgttggtcc cgtcgcgatt ttcttctccg tttgcttcat tgtgtttatg gttgaaacaa
    94741 acgcagtcgc gaagcgtaaa accgtcatgc gcggtgacga gattaatcgc ggcactcggc
    94801 agacgaccat tacgtttaaa aacatcgctg gaggcagcaa aacgcccggc aaacgccccc
    94861 agaggcaaat cataatgtag ccagaaacga cgggcagcat cgcggaaatg atcgttccac
    94921 tcggcaaaca gcggcgggaa atttcccacc tgataaccac caggagcgat atcccacggt
    94981 tcagcaatta acttcacctg cgagagcacc gggcagttct ggatagcggt aaacaacggc
    95041 gcatcctgac ggaactctgg cgtacggccc atgactgccg ccagatcaaa gcggaaacca
    95101 tcgacgtggc aggtttctac ccaataacgc aggcaggcgc tggcataatc caccaccgcc
    95161 gggtgactca aattgagcgt gttgccacaa ccggtccagt tgtgataatc gccgtcttct
    95221 cttatccaat aatagctacg gttgtcgatc ccacgcagcg agaataacgg gccgtcgagg
    95281 tccagttccg cactatggtt gagcacgata tcaagaatga cttcgatacc cgctttatgc
    95341 agtgctttga ttgcatcgcg aaactcatgc agcgccgttt ctggcgagca ggcatacgcc
    95401 ggatgcagcg caaacatcgc caccgggttg taaccccagt agttacttag ccccatgcgt
    95461 tgcagacgtg gttcactggc aaactgcgcc actggcagca gttccagcgc ggtaatgccc
    95521 aattgtttca aatagttgat catcaccgga tgcccgaggg ctttataagt gccacggatc
    95581 tcgaccggga tctccgggtg caggtacgtt aatcctttaa catgggcttc ataaatgatg
    95641 gtgctgcccc acggcgtgcg cggcggggca tcatcttccc agtcatagtg atcaaccacc
    95701 actacgcatt tcggcgcaat ggcggcgttg tcgcgatagt caggttcatt atgaccggcg
    95761 tgcagcagcg ggttatcttt aaactccccg tcaatttgcc gcgcgcaagg atcaatcaac
    95821 aacttcgccg ggttaaagcg atgcccctcg gcgggttgcc aggggccatg aacgcgataa
    95881 ccataacgca aacccgggcg cgcatccggc agataaccgt gccaaatgtc gccactgtgc
    95941 cctggcaagt catagcgatg ttcctggcca ttggcgtcaa agacacacag ttctacccgc
    96001 tcggcatgag cggagaaaag tgtgaagttg acgccctgac cgtcgtaatg cgcgccgagg
    96061 ggagcgggtt tgccaatggc gagttgtgtc attctgcctc ccgaaccagc cagatagtgg
    96121 ccagcggtgg tagcgtcagg cttagtgaat gctgacgacc gtggctggca atctcatcgc
    96181 tgtgtaccgt gccgccatta cctgcattac tgccgtgata gtgcatggaa tcggtattga
    96241 ggatctcacg ccatttgcct ggctgattaa tgccgaagcg ataatcatga cgcggcaccg
    96301 gcgtaaagtt actggcaacg atgatttcgt taccctcttt atcgcgacgc acaaagatca
    96361 gcaccgagcg ttctttgtca tccaccacca gccattcaaa gccgtacggg tcaaaatcca
    96421 gttcatgcat cgctttatgg tggcggtagg tgaggttcag atcgcgcacc agacgctgga
    96481 caccgtggtg ccagttatcg ccgccttcca acagatgcca gtcgaggctg gcgtcatggt
    96541 tccactcgcg gccctgggca aattcgttcc ccatgaacag cagtttcttg cccggaaatg
    96601 cccacatcca gccatagtaa gcgcgcaggt tagcgaattt ctgccatgcg tcgcccggca
    96661 tgcggtcgag aatcgatttt ttaccgtgga ccacttcatc atgcgacaac ggcaggacga
    96721 agttttcagt gtagttgtag agcatcccga aggtcagttt atcgtgatga tactgacgat
    96781 aaatcgggtc gagcttcatg tagtccaggg tgtcatgcat ccagccgagg ttccacttgt
    96841 accagaagcc cagaccgccc atatcctgcg gacgagaaac gccagggaaa tcggtagact
    96901 cctcagccat tgtcaccgca ccggaaacct gctcaccaag aatacggttg gtattacgca
    96961 agaattcaat cgcttcaaga ttctcgcgcc cgccaaattc gttcgggatc cactccccct
    97021 ctttacggct gtagtcacga taaatcatcg acgccaccgc atcgacgcgc agcgcatcaa
    97081 taccaaaacg ttcaatccag taaagcgcgt taccgacgag gaagttactg acttcacggc
    97141 gaccatagtt gtaaatcagc gtgttccagt cctgatgata gccttcgcgc ggatcgctgt
    97201 gttcatacaa gttcgtacca tcaaattcgg caagcgcaaa gtcatcagtc gggaagtggc
    97261 ctggcaccca gtcgagaatc acgttcagac cagctgcgtg tgcagcatca atgaaataac
    97321 ggaagtcgtc gcgagtacca aaacggcggg ttggtgcata caggccggtt ggctgataac
    97381 cccaactgcc atcgaaggga tgctcgttaa ggggcagtag ttcgaggtgg gtaaagccca
    97441 tccatttagc ataaggcacc agttgatcgg ccagctcgcg gtagctcaac cagaaattgt
    97501 tgtcggtgtg gcgacgccag gagcccaggt gaacttcata aatagagatt ggcgcatcaa
    97561 actgattcgc ttttttgcgc tcttcagtct gtacaacctt ttccggcagc ccgcaaataa
    97621 gagacgcggt ttccgggcgc atttgcgctt cgaaggcata agggtcggac ttcagacgca
    97681 agttgccatt ggcatcaatc atctcgtatt tatagagctg accgttatgc gccccaggga
    97741 taaacagttc ccagatgccg ctctctttac gcaggcgcat cgggtgacgg cgaccgtccc
    97801 agtagttgaa ttgcccaacc accgagaccc gacgggcgtt tggagcccag acagagaaac
    97861 gcgtacctgt gacgccatcc atagtatctg catgcgcgcc taaagtttca tacgggcgca
    97921 ggtgagtacc ttcagataat agccaggcat ccatttcctg gattagcgga ccaaaacggt
    97981 aaggatcatc aatcaggttt tgctgcccat gccagacaac agccaactga tagcggaaaa
    98041 aattcttacg tcgcggaatg acgccactaa agaatccccg tgagtcgaga cactccagtt
    98101 ttgcgagttt gcgcccggtt ttcggttcaa tcacccacac atcggtagcg tcgggtaaaa
    98161 gggcacggac ttccagtccc gcggtggttt tatgcattcc cagtacggaa aaaggatccg
    98221 caaaatggcc tgcaattagc gcgttaatca cgtctctatc gatacgatcg gacatgcttg
    98281 tcttcctgtt ttattgtgtc accccatcca attggatttt tgacttctgg ttgtgacatt
    98341 tttttgacct gaacggcgca gcactctgtg catcctctct gcgtcgtcct cacttcaggt
    98401 aaggctgtga atactcatgt attcagccac ccttaaagaa tagccaatgc tctatttaac
    98461 tcccggtaaa tcatgaaaca tctgcgctta ctcctgtatt acgcactaac aggggcggca
    98521 tcgcgcccca gatttaatga ataaagatta cgccagttga cgaagcatcc gacgcagcgg
    98581 ctccgcggcc ccccacagca gctggtcgcc cacggtaaag gctgacagga actctggtcc
    98641 catattcagc ttacgcaggc ggcctaccgg cgtggtcagc gtgccggtaa cggcagctgg
    98701 ggttagctca cgcatagtga tttcccgatc gttcggaacg actttcgccc acggattgtg
    98761 cgcagccagc agttcttcca cggtcggaat cgacacatct tttttcaatt taatagtgaa
    98821 tgcctggctg tggcagcgca atgccccgac acgcacacat aaaccatcta ccggaattac
    98881 ggaagatgtg ttgaggatct tgttggtttc cgcctgccct ttccactctt cgcggctctg
    98941 accgttatcg agctgtttgt cgatccacgg aatcaggcta cccgccagcg gcacgccaaa
    99001 gttatccacc ggcagctcac cgctacgggt taaggttgtg actttgcgtt cgatatcgag
    99061 aatagcagag gacggggtcg cgagttcatc tgctacatgg ccatacagat ggcccatctg
    99121 ggttaataac tcacgcatat gccgcgcacc accgccggaa gcggcctggt aggttgcaac
    99181 ggacacccaa tcaacaagat cattggcgaa taaaccaccc aacgacatca acatcaggct
    99241 tacggtacag ttaccgccaa caaaagtcct gatgccatta tttaatccgt cggtaatgac
    99301 gtcctgattg acggggtcaa gaatgatgat ggcgtcatct ttcatgcgca gagacgatgc
    99361 tgcgtcaatc cagtaaccct gccatccgct ttcacgaagc tttggataga tttcgttggt
    99421 ataatcgccg ccctgacagg tcacaatgat atcgagggcc tttagcgcct ccagatcaaa
    99481 ggcatcctga agtgtgccag tggttccgcc aaaagacggc gcagcctgac caagctgaga
    99541 agtagaaaag aagacagggc gaatggcgtc gaagtcgcgc tcttcaacca tgcgttgcat
    99601 gagaacggag ccgaccatac cgcgccagcc gataaaacca acatttttca taagcgtttt
    99661 ttcctgcaaa gatgtgtgct gtataaatgt gccggtctcc tcttggcaca tctttcacca
    99721 tacaaaaagc agccaaagtc gcaagtgaaa ttaatcaatg atagcgaagc catcagtaat
    99781 gcgacttatc ctgctttgtt agcacgcaga aagtccgcgg caattatcag ggaatttgag
    99841 ttatgaatga aatcatttct gcagcagttt tattgatcct gattatggat ccgctcggaa
    99901 acctacctat tttcatgtcc gtactgaaac atactgaacc gaaaagacgg cgggcaatca
    99961 tggtgcgaga gttgcttatt gctctcctgg tgatgctggt gttcctgttt gcgggtgaga
   100021 aaattctggc atttctcagc ctgcgagcag aaaccgtctc catttctggc ggcatcattc
   100081 tgtttctaat tgccattaag atgattttcc ccagcgcttc aggaaatagc agcgggcttc
   100141 cggcaggtga agagccattt atcgtgccgc tggcaattcc gctggtcgcc gggccaacta
   100201 ttctcgccac gctgatgttg ttgtctcatc agtacccgaa tcagatggga catctggtga
   100261 ttgctctgct gctggcctgg ggcggcacct ttgtcatcct gctacagtct tcgctatttt
   100321 tacgtctgct gggcgagaaa ggggtgaacg cacttgaacg cctgatggga ttgattctgg
   100381 tgatgatggc aacccagatg ttcctcgacg gcattcgaat gtggatgaag gggtaaggtt
   100441 caaacaggtg ctgcacacaa ccagacgcgg cacctgtttt attacctgaa ctgataatga
   100501 ccttcgccca atcggataac ccttccctct tcttctgcgc ggttcagcca cacactaatt
   100561 tgttcgatgg ttaaaccagt acaagacgcc agcctttcaa tacttatcga ttcctttgca
   100621 aggatcgcca gttccgccac aaagagttga taaaaatcga caggcgtata cgcgggcatt
   100681 actgcgtaat ttgcgtcatc ttcatgcagc gaaaacagtt ctggttgagc gttacggagt
   100741 gctctgtcat acatcagccc aggaacgtct ggcagtctga tcgtcacatt ctcagcctgt
   100801 tcagcactcc atgacgcccc attttctact aatcgtgaat tggctgattg catatcctga
   100861 tttggtttaa cctgcacagg cagccactga tgttttaatg cctccatcgc cccggttatc
   100921 gttccaccgg tcattcccgc acgtacaacc attgcgcttt cagcaaggca ataaatatat
   100981 ttatttcgcg ccatcgcatt gccgacggtg aaacgtacct ctgggtaaaa cggcgaaatc
   101041 aacaccaggt tgcctgctat aagcccttca cgccatttca ctaacgtact cgtttttaac
   101101 aagctatcag ctaatacgcc aacggcagtt cccccggcct ccagtgccga cgccattgca
   101161 cattcatcaa tacctcgcgc accaccagag ataacgcaaa tcccctgttg ggccagtttc
   101221 gcggccagtt gttgggtata gcgcaaatcg tcagtcggag catctcgcga gccaacaatc
   101281 gccatacctt ccgcttgcag taatgcttta ttcccgcagc caaataaaac gggaggtgca
   101341 tccgttcgca atcggttttt caagcgaacg ggataatcag catctccgcg ggttaagatc
   101401 cataaaccgg cgcgttgcca cttatctacc gccaacgcca gactatgacc acgcgccagc
   101461 aagccaagaa tacgctcctg agaaatacgc ggatcgctcc agcctgtaag tttctcttgc
   101521 ggttgcggca ccagaagctc ggcgggactg atacgttggt gcttcagcca taatgcaaac
   101581 ttcccccatt cactattact aagaggttta tacttactcg ccgccgcgcg agaaaaatcg
   101641 ctggttagca ggagagttgc ttgtgcattg gctgaaagat tcatcaattt tttaccgagg
   101701 tagaagcaag ggcaagagga taaaccgtcg ggcaacctgc ctggcgtaac agtgctgtta
   101761 gaactgtcag cgtccatgca gaatccacga tatcgtcaac cagcaacgcc ggaccaggca
   101821 tcaaaggagg ggtaatcaca aacgccccgt cgagattttg acactggtgg aaacggtttt
   101881 gctgcatttt ctgcggtgga ttgtccacga ctttttcaat ggcatcaata aaaggtaagc
   101941 caagtttcgc cgccagtcgc cgggcaaaat caggaaccag gtcgaggtgc tttcaaaatc
   102001 aggaaccagg tcgaggtgct ttaatgaagg aacgcagcat acccaggttg gttcaggatg
   102061 cggattccag cgttggcgaa ccatttccgc acatgcctcc accagttcat cactgaatcg
   102121 ccccgttttt ttctcctgtg ctacctgctt tccccacccg gaatatcccc aacgagaaag
   102181 gattcgcccc gtagatcctt gtaaactcgc aggcaagttc cctttaaatc cgtattgcgt
   102241 aaaagcccca gctgccacct gtttattgag atttaatgac aggtcagcgt gtttaataaa
   102301 taagtttgcc gcatggagta agccgctatc aatgtcgggc gacaacaacg gatgctgaag
   102361 acaactgcta catttaccac aatgttcggc acctggctca tcgagtgcgc ggcgcagaaa
   102421 ttgcattttg cactccttgg tcgtgatata gctttcaacg tctgccagct cactttttct
   102481 ctgattcatc aaatggttaa ttcgttcatg gtcaggagaa aaactgacaa ttgttctgcg
   102541 ccataatttc tcggtataca ccacaggcga tggattttca gctaccaata atttcagtgc
   102601 tttttctatt tgcccataac ggagattcgt ccgttgttca atgcctcgta aggtaagacc
   102661 gtcattcacg ctaagtacgt tgagtatttc atgaatttgc gcctccgcag gaaaggcgct
   102721 ttcacggaag aatttatgaa tagcacggtc ttcactgccg caaagcagta tgccaacagc
   102781 agaatcgata ccgcgaccgg cacgccccac ctgttagtag taaccaacta tcgaacctgg
   102841 catctggtaa tgaataacaa accccaaatc cggtttgtca aagcccattc ccagcgccgt
   102901 tgtcgcaacc agcactttaa ttttatttgc cagcaacgcc tgctccagat attccctggc
   102961 agtattagaa tcttccgcgc cttcacaagt cacgccgctg tagtaagcaa atgcactgat
   103021 accatttttc cttaaccact cggcgacaag ttcggcatcg cgagtagtca gggtataaac
   103081 aattcccgat ttggaaaact gagggataac cgttgctaac catgccagac gggatgagtg
   103141 ttcacctaag actaaggcat caagcgccaa actttcgcga gccagcgttc cacgctgaat
   103201 cacaatgtca cccaattgct gacggatatc ctcaacgaca cggttattcg ctgtcgcggt
   103261 tgtacccaga acaggggtat tcgcaggtag ttgcgcaaaa tatctaatat acgtcggtaa
   103321 tccgggcgaa aatcgtggcc ccaatcagag atgcagtgcg cctcgtcgac cacaagcaag
   103381 ccaatatgat cggctatagg atataaaacc gtttcgataa agtcctggtt tgccaaacgt
   103441 tccggggaaa ccagcaagca atcgacctca ccttgcagca acttatcgct aatgcgctgc
   103501 cattcttctc tgtttgtaga attcaacgtt tcagccgtaa tacctaaccg ttcagcggcg
   103561 gcaacctggt tacgcatcag ggcaagcaaa ggagagatga taattgtcgg gccagcgccg
   103621 cgatcgcgga agattttact ggcgatgaag tacacagcac ttttccccca gccagtgcgt
   103681 tggacaacca gcagttttcg ccgttggttc accaccgcgt ctatggcttc ccattgccct
   103741 tccctgaaag tagcggtggt atttcctaac atccgctgta aaagcaattc agctgcgtgc
   103801 ttttccatct ttatctcctg cgatctttag gcagtgatta gaacggatta tttgcaggta
   103861 aatttgcgag agagatcaaa aagatgaaga gaggagaaaa gagaaatgaa ttactgcgag
   103921 caagctcgca gtaatttgtc gcagtaatta actcaacagc tggaacgcaa tcatcccaac
   103981 gatggcaccg acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc
   104041 ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa cgtggctgac
   104101 aacaatcgaa ccaccggcga tacaaatcga cagcgccgcc atttgcgcac cggagtagtt
   104161 cagttgttca ataaccggca tcaccagtcc caccgccgtt aaacaggcaa cggttgcaga
   104221 accctgaatg atgcgcactg cagcggccag cacgaagcag gtgatggcaa tcggcaggcc
   104281 catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca gcacctgttt
   104341 gaacacgccg cccgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc
   104401 gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc cataaatcgc
   104461 taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga accattcgta
   104521 agcggtagag ccttccggca caaaacgcgc ggcaatggtt ttcagcccta ccagcaccag
   104581 cggcagcagg atcagcgaca ggctgaatcc gaaggatggc attttgcctt cgccgagatg
   104641 cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga agttacccca
   104701 cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca ggatcatcca
   104761 gccaaaatcg gcgttcatct gcgatgccag cagcattggc gctggccctg gcaccaggaa
   104821 cgccgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca ggttcgtacc
   104881 ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca cttcaaagaa
   104941 cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc ggctgtgacc
   105001 gaaggattta agcattttga cggcaatctg atcgactgcg ccggtttcat gtaagatctt
   105061 gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc
   105121 tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat
   105181 ggacaccacc attaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag
   105241 cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc
   105301 ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac
   105361 caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa
   105421 catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cggctttcaa tcacatcaaa
   105481 atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca agtcacgata
   105541 gtgttttttc aatgcagaac agacgatcag cgacacttta ttagtgcgct gcatagcaaa
   105601 cgcggcgtcg ttcagcgcct gcaaccacgg tttgcgatcg tcgtcattca gcggttcgcc
   105661 agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa gaaacgcggc
   105721 atgaagttga tgcgccactt cacttgcgac cgcagatttg ccgctgcccg atacgcccat
   105781 caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa
   105841 gaattaaaaa tgttacgggt aactgttatc ggtaacattg tccagccgga caatatcaga
   105901 agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtgggctta
   105961 aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc
   106021 gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct cacgcggcgt
   106081 cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt ggaaaccggc
   106141 aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg ccgcgccgac
   106201 cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc gccgtgcctg
   106261 gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca cgctatatgg
   106321 caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga tagtacgttc
   106381 gtcgagacgt gcgccgagat aggcaatgtg gcgatgcccg cgagcaataa tggctgtggt
   106441 catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgagac acggcgactg
   106501 gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta aggtgcgcgg
   106561 cgtgtgggta cgttcggtga ggatcagtcc gtcgatattc catgagagca ttgattcgag
   106621 acgttcttgc tccatttccg gtttataacc gtagtgggcc agcatggtct gataaccgtg
   106681 cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga
   106741 aggtaacagg acgccaatcg cccggctggt ggcgttagag aggatatcgg gcgcacgatt
   106801 gggaatatag cccagttcat caagagccgc ggcaatcttg ccgcgtagag cgacggaaac
   106861 ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta cacggtcagc
   106921 cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat
   106981 tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa
   107041 ttattagata tacactaaat caattgcatc gcattgtgct aacgacgctg caaaaccccg
   107101 gaagcgtaca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca
   107161 aagatgccgt tgagctttaa accggcggca gatcgaacag taacacttcg ctgtcgctat
   107221 ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg atcgaggctt
   107281 tcacgccgtt aatggtgaca ttgcctttca ccacctggat ccagacgcgg cgttcagcgg
   107341 caatctgatg caccgactgc tcatctttca gcaacgccca gcggtacagt tccatatcct
   107401 gatgcacttt caatgaacca tcacgcgcat ctggcgaaag caccagttgt ttgccctgca
   107461 ctgcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc gttttcttcc ggcatgatcc
   107521 agatctgata caggtgcaga cgctcggtgc tgcttgggtt gtactctgag tgacgaatac
   107581 ccgtacccgc actcataatc tggaactcgc ccgccggaac ctgctcttta ttgcccatgc
   107641 tgtcctggtg ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
   107701 ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg cgcagcgcgg
   107761 agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc caggagtcca
   107821 gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc atttgtattc
   107881 gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg
   107941 tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttactt
   108001 cgcgagagtc actgtggagg gagaggcttg ctcgaatcca cgttcaagta tttccagatt
   108061 cgtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg tttgatacaa
   108121 cgcatcataa acgcgcccgt aatcgcccat ctccggcttc atctcttctc tgaccgtcac
   108181 gccctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga atcccggttc
   108241 gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccat atttaataaa
   108301 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
   108361 tttgacgatg gcttttagat cgccataaaa cagttgcgct tcgaaagtat cgtcaggatt
   108421 ggctttattg cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
   108481 aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct gcggcagccc
   108541 aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt cgacaatctc
   108601 tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat caaagcgacg
   108661 attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca gctctttcgc
   108721 ctgcgcaagt gtcggagtga acggtttttc gaccagcaca tttttcccgg cttccatcgc
   108781 gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca gcttaacatc
   108841 gggatcgttt agcacttcgt tgagatcgct ggtgaaatgg atatgagagt aaatgggagc
   108901 ttgttcttcc ggctttgcat ggcggcgaaa aatatgcgcg acatgccagc tatccttgcg
   108961 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
   109021 gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
   109081 cgtggttcct actggagtgc gcataacgaa cacaagcact tcccgaggat aaattgagaa
   109141 cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa gcaatgtgag
   109201 caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgagaca ataataatca
   109261 ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc gcattcgaaa
   109321 gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag tgagatagtg
   109381 atacgccatg ctgaaacacg ggattacgag gccatcaggc agattcacgc ccagccggag
   109441 gtgtattaca acacactaca ggtgcctcat ccttccgatc atatgtggca ggagcgactc
   109501 gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt cgtgggccat
   109561 ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt tggtatctgt
   109621 gtcgacgccc gctggaagaa tcgcggcgtc gccagcgccc tgatgcgaga gatgattgaa
   109681 atgtgcgaca actggttgcg ggtagatcgt attgagctaa cggtgttcgt cgataacgcg
   109741 ccagcaatta aggtctataa aaaattcggc tttgagattg aagggactgg caagaagtac
   109801 gcattacgta atggtgaata tgttgatgcg tattttatgg cgcgggtgaa gtaaagaata
   109861 ttctcaaatt gcctgttacc aatgtgaata attagttttg ttcatctaat ttgagttttt
   109921 ccttatatta ccaccatcat tttgaaacaa tttgtaacat c
//
LOCUS       DAZFDC010000003       100486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFDC010000003 DAZFDC010000000
VERSION     DAZFDC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.003"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(138..713)
                     /gene="gmhB"
                     /locus_tag="ACHJ48_000213"
     CDS             complement(138..713)
                     /gene="gmhB"
                     /locus_tag="ACHJ48_000213"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO4705848.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            901..1932
                     /gene="metN"
                     /locus_tag="ACHJ48_000214"
     CDS             901..1932
                     /gene="metN"
                     /locus_tag="ACHJ48_000214"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO4705849.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            1925..2578
                     /gene="metI"
                     /locus_tag="ACHJ48_000215"
     CDS             1925..2578
                     /gene="metI"
                     /locus_tag="ACHJ48_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO4705850.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVVGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            2618..3433
                     /gene="metQ"
                     /locus_tag="ACHJ48_000216"
     CDS             2618..3433
                     /gene="metQ"
                     /locus_tag="ACHJ48_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO4705851.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            3551..3955
                     /gene="rcsF"
                     /locus_tag="ACHJ48_000217"
     CDS             3551..3955
                     /gene="rcsF"
                     /locus_tag="ACHJ48_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO4705852.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQALCIGSALNITAK"
     gene            3952..4659
                     /gene="tsaA"
                     /locus_tag="ACHJ48_000218"
     CDS             3952..4659
                     /gene="tsaA"
                     /locus_tag="ACHJ48_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO4705853.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDCVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDEGFEVFALEPR"
     gene            4770..6488
                     /gene="proS"
                     /locus_tag="ACHJ48_000219"
     CDS             4770..6488
                     /gene="proS"
                     /locus_tag="ACHJ48_000219"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO4705854.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFTAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            6541..7365
                     /gene="yaeF"
                     /locus_tag="ACHJ48_000220"
     CDS             6541..7365
                     /gene="yaeF"
                     /locus_tag="ACHJ48_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO4705855.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLEVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVVEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(7565..8275)
                     /gene="nlpE"
                     /locus_tag="ACHJ48_000221"
                     /gene_synonym="cutF"
     CDS             complement(7565..8275)
                     /gene="nlpE"
                     /locus_tag="ACHJ48_000221"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO4705856.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(8289..8699)
                     /gene="arfB"
                     /locus_tag="ACHJ48_000222"
     CDS             complement(8289..8699)
                     /gene="arfB"
                     /locus_tag="ACHJ48_000222"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO4705857.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRSGRE"
     gene            complement(8696..9241)
                     /gene="yaeQ"
                     /locus_tag="ACHJ48_000223"
     CDS             complement(8696..9241)
                     /gene="yaeQ"
                     /locus_tag="ACHJ48_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO4705858.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            9407..9607
                     /gene="yaeP"
                     /locus_tag="ACHJ48_000224"
     CDS             9407..9607
                     /gene="yaeP"
                     /locus_tag="ACHJ48_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO4705859.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            9594..9854
                     /gene="rof"
                     /locus_tag="ACHJ48_000225"
     CDS             9594..9854
                     /gene="rof"
                     /locus_tag="ACHJ48_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO4705860.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(9907..11202)
                     /gene="tilS"
                     /locus_tag="ACHJ48_000226"
     CDS             complement(9907..11202)
                     /gene="tilS"
                     /locus_tag="ACHJ48_000226"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO4705861.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGGAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GQGSVQLNAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGGFVTQEGVAEGENGISFVWQRNA"
     gene            complement(11267..11656)
                     /gene="yaeR"
                     /locus_tag="ACHJ48_000227"
     CDS             complement(11267..11656)
                     /gene="yaeR"
                     /locus_tag="ACHJ48_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4705862.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(11713..13854)
                     /gene="ldcC"
                     /locus_tag="ACHJ48_000228"
     CDS             complement(11713..13854)
                     /gene="ldcC"
                     /locus_tag="ACHJ48_000228"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO4705863.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALCF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            complement(13953..14912)
                     /gene="accA"
                     /locus_tag="ACHJ48_000229"
     CDS             complement(13953..14912)
                     /gene="accA"
                     /locus_tag="ACHJ48_000229"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO4705864.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(14925..18407)
                     /gene="dnaE"
                     /locus_tag="ACHJ48_000230"
     CDS             complement(14925..18407)
                     /gene="dnaE"
                     /locus_tag="ACHJ48_000230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO4705865.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQHLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(18444..19040)
                     /gene="rnhB"
                     /locus_tag="ACHJ48_000231"
     CDS             complement(18444..19040)
                     /gene="rnhB"
                     /locus_tag="ACHJ48_000231"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO4705866.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(19037..20185)
                     /gene="lpxB"
                     /locus_tag="ACHJ48_000232"
     CDS             complement(19037..20185)
                     /gene="lpxB"
                     /locus_tag="ACHJ48_000232"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO4705867.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NIILEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNSARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(20185..20973)
                     /gene="lpxA"
                     /locus_tag="ACHJ48_000233"
     CDS             complement(20185..20973)
                     /gene="lpxA"
                     /locus_tag="ACHJ48_000233"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO4705868.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMAAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(20977..21432)
                     /gene="fabZ"
                     /locus_tag="ACHJ48_000234"
     CDS             complement(20977..21432)
                     /gene="fabZ"
                     /locus_tag="ACHJ48_000234"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO4705869.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(21537..22562)
                     /gene="lpxD"
                     /locus_tag="ACHJ48_000235"
     CDS             complement(21537..22562)
                     /gene="lpxD"
                     /locus_tag="ACHJ48_000235"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO4705870.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKLLE
                     RKVNQQD"
     gene            complement(22566..23051)
                     /gene="skp"
                     /locus_tag="ACHJ48_000236"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(22566..23051)
                     /gene="skp"
                     /locus_tag="ACHJ48_000236"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO4705871.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(23172..25604)
                     /gene="bamA"
                     /locus_tag="ACHJ48_000237"
     CDS             complement(23172..25604)
                     /gene="bamA"
                     /locus_tag="ACHJ48_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO4705872.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(25634..26986)
                     /gene="rseP"
                     /locus_tag="ACHJ48_000238"
     CDS             complement(25634..26986)
                     /gene="rseP"
                     /locus_tag="ACHJ48_000238"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO4705873.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(26998..27855)
                     /gene="cdsA"
                     /locus_tag="ACHJ48_000239"
     CDS             complement(26998..27855)
                     /gene="cdsA"
                     /locus_tag="ACHJ48_000239"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4705874.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(27868..28626)
                     /gene="ispU"
                     /locus_tag="ACHJ48_000240"
                     /gene_synonym="uppS"
     CDS             complement(27868..28626)
                     /gene="ispU"
                     /locus_tag="ACHJ48_000240"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO4705875.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(28815..30011)
                     /gene="ispC"
                     /locus_tag="ACHJ48_000241"
     CDS             complement(28815..30011)
                     /gene="ispC"
                     /locus_tag="ACHJ48_000241"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO4705876.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDASAREVARKE
                     VMRLAS"
     gene            complement(30103..30660)
                     /gene="frr"
                     /locus_tag="ACHJ48_000242"
     CDS             complement(30103..30660)
                     /gene="frr"
                     /locus_tag="ACHJ48_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO4705877.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            complement(30810..31535)
                     /gene="pyrH"
                     /locus_tag="ACHJ48_000243"
     CDS             complement(30810..31535)
                     /gene="pyrH"
                     /locus_tag="ACHJ48_000243"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO4705878.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            complement(31682..32533)
                     /gene="tsf"
                     /locus_tag="ACHJ48_000244"
     CDS             complement(31682..32533)
                     /gene="tsf"
                     /locus_tag="ACHJ48_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO4705879.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            complement(32791..33516)
                     /gene="rpsB"
                     /locus_tag="ACHJ48_000245"
     CDS             complement(32791..33516)
                     /gene="rpsB"
                     /locus_tag="ACHJ48_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO4705880.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33884..34678
                     /gene="map"
                     /locus_tag="ACHJ48_000246"
     CDS             33884..34678
                     /gene="map"
                     /locus_tag="ACHJ48_000246"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO4705881.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            34740..37412
                     /gene="glnD"
                     /locus_tag="ACHJ48_000247"
     CDS             34740..37412
                     /gene="glnD"
                     /locus_tag="ACHJ48_000247"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO4705882.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVNVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEVLHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            37443..38267
                     /gene="dapD"
                     /locus_tag="ACHJ48_000248"
     CDS             37443..38267
                     /gene="dapD"
                     /locus_tag="ACHJ48_000248"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO4705883.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGDIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            38582..38968
                     /gene="yaeH"
                     /locus_tag="ACHJ48_000249"
     CDS             38582..38968
                     /gene="yaeH"
                     /locus_tag="ACHJ48_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO4705884.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(39057..40214)
                     /gene="cdaR"
                     /locus_tag="ACHJ48_000250"
     CDS             complement(39057..40214)
                     /gene="cdaR"
                     /locus_tag="ACHJ48_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO4705885.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDVEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(40369..41793)
                     /gene="degP"
                     /locus_tag="ACHJ48_000251"
     CDS             complement(40369..41793)
                     /gene="degP"
                     /locus_tag="ACHJ48_000251"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO4705886.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            complement(41923..43440)
                     /gene="dgt"
                     /locus_tag="ACHJ48_000252"
     CDS             complement(41923..43440)
                     /gene="dgt"
                     /locus_tag="ACHJ48_000252"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO4705887.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAVL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRSPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            43524..44222
                     /gene="mtnN"
                     /locus_tag="ACHJ48_000253"
     CDS             43524..44222
                     /gene="mtnN"
                     /locus_tag="ACHJ48_000253"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO4705888.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            44215..45015
                     /gene="btuF"
                     /locus_tag="ACHJ48_000254"
     CDS             44215..45015
                     /gene="btuF"
                     /locus_tag="ACHJ48_000254"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO4705889.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATNIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            45053..45676
                     /gene="yadS"
                     /locus_tag="ACHJ48_000255"
     CDS             45053..45676
                     /gene="yadS"
                     /locus_tag="ACHJ48_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO4705890.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRHLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(45723..46067)
                     /gene="erpA"
                     /locus_tag="ACHJ48_000256"
     CDS             complement(45723..46067)
                     /gene="erpA"
                     /locus_tag="ACHJ48_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO4705891.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(46060..46125)
                     /gene="yadW"
                     /locus_tag="ACHJ48_000257"
     CDS             complement(46060..46125)
                     /gene="yadW"
                     /locus_tag="ACHJ48_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO4705892.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(46149..47570)
                     /gene="clcA"
                     /locus_tag="ACHJ48_000258"
     CDS             complement(46149..47570)
                     /gene="clcA"
                     /locus_tag="ACHJ48_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO4705893.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            complement(47582..47629)
                     /gene="yadX"
                     /locus_tag="ACHJ48_000259"
     CDS             complement(47582..47629)
                     /gene="yadX"
                     /locus_tag="ACHJ48_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO4705894.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            47795..49075
                     /gene="hemL"
                     /locus_tag="ACHJ48_000260"
     CDS             47795..49075
                     /gene="hemL"
                     /locus_tag="ACHJ48_000260"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO4705895.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(49110..51092)
                     /gene="fhuB"
                     /locus_tag="ACHJ48_000261"
     CDS             complement(49110..51092)
                     /gene="fhuB"
                     /locus_tag="ACHJ48_000261"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO4705896.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(51089..51979)
                     /gene="fhuD"
                     /locus_tag="ACHJ48_000262"
     CDS             complement(51089..51979)
                     /gene="fhuD"
                     /locus_tag="ACHJ48_000262"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO4705897.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMNPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(51979..52776)
                     /gene="fhuC"
                     /locus_tag="ACHJ48_000263"
     CDS             complement(51979..52776)
                     /gene="fhuC"
                     /locus_tag="ACHJ48_000263"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO4705898.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(52827..55070)
                     /gene="fhuA"
                     /locus_tag="ACHJ48_000264"
     CDS             complement(52827..55070)
                     /gene="fhuA"
                     /locus_tag="ACHJ48_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO4705899.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            complement(55290..57824)
                     /gene="mrcB"
                     /locus_tag="ACHJ48_000265"
     CDS             complement(55290..57824)
                     /gene="mrcB"
                     /locus_tag="ACHJ48_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO4705900.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(58020..60449)
                     /gene="hrpB"
                     /locus_tag="ACHJ48_000266"
     CDS             complement(58020..60449)
                     /gene="hrpB"
                     /locus_tag="ACHJ48_000266"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO4705901.1"
                     /translation="MSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERATAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            60523..61053
                     /gene="thpR"
                     /locus_tag="ACHJ48_000267"
     CDS             60523..61053
                     /gene="thpR"
                     /locus_tag="ACHJ48_000267"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO4705902.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWVLTQ"
     gene            61068..61772
                     /gene="sfsA"
                     /locus_tag="ACHJ48_000268"
     CDS             61068..61772
                     /gene="sfsA"
                     /locus_tag="ACHJ48_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO4705903.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            61950..62405
                     /gene="dksA"
                     /locus_tag="ACHJ48_000269"
     CDS             61950..62405
                     /gene="dksA"
                     /locus_tag="ACHJ48_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO4705904.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            62472..63368
                     /gene="gluQRS"
                     /locus_tag="ACHJ48_000270"
     CDS             62472..63368
                     /gene="gluQRS"
                     /locus_tag="ACHJ48_000270"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO4705905.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            63428..64825
                     /gene="pcnB"
                     /locus_tag="ACHJ48_000271"
     CDS             63428..64825
                     /gene="pcnB"
                     /locus_tag="ACHJ48_000271"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO4705906.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            64822..65304
                     /gene="folK"
                     /locus_tag="ACHJ48_000272"
     CDS             64822..65304
                     /gene="folK"
                     /locus_tag="ACHJ48_000272"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO4705907.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQQIELQQGRVRKAERWGPRTLDLDIMLF
                     GNETINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQPAIWY
                     "
     gene            65585..66181
                     /locus_tag="ACHJ48_000273"
     CDS             65585..66181
                     /locus_tag="ACHJ48_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4705908.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            66268..66993
                     /locus_tag="ACHJ48_000274"
     CDS             66268..66993
                     /locus_tag="ACHJ48_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO4705909.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVTPFSQASFGVTSLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            67035..69635
                     /locus_tag="ACHJ48_000275"
     CDS             67035..69635
                     /locus_tag="ACHJ48_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO4705910.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSSNGAAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGQATSSLEIPFVDIGENSAAACLTHKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVCFDGSDQFLDLTIPQAYVLKSYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNDNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQSGYKIYETTVPPGEFVIDDISPSGFGSELVVTIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDSLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTYYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINALGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQG
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTQGSTNTLLL
                     NNLTCQVANH"
     gene            69649..70197
                     /locus_tag="ACHJ48_000276"
     CDS             69649..70197
                     /locus_tag="ACHJ48_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4705911.1"
                     /translation="MKRILLTSALIGLGLPAVGSATDLNVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVATAAPEAQANFKLKASDCSNGYSKIFTTLTGTTV
                     SGKLIVNEATSGAAGVGMGIKRRDTADSTFFTPNNTDKFEWSADEKASGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            70212..70817
                     /locus_tag="ACHJ48_000277"
     CDS             70212..70817
                     /locus_tag="ACHJ48_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4705912.1"
                     /translation="MKLKVIATLIATVAVGVSFNSNFASASTTSASLTVNSNLTMGTC
                     SAQIMDNSNKVINEVVFGNVYISELGAKSKVQQFKIRFSNCSGLPQNSAQIVLAPNGI
                     SCAGSQSSSAGFSNKFTDASAATRTAVEVWTTDTPESNGSTQFHCAQKIPVPVTLPAD
                     TTTQPYDYPLSARMTVAEGRLVTDVRPGNFRSPTTFTITYQ"
     gene            70838..71428
                     /locus_tag="ACHJ48_000278"
     CDS             70838..71428
                     /locus_tag="ACHJ48_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO4705913.1"
                     /translation="MLCRHHKIVHFLGLATALITPFAYSGQDVDLTAKIVPSTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            71440..72549
                     /locus_tag="ACHJ48_000279"
     CDS             71440..72549
                     /locus_tag="ACHJ48_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4705914.1"
                     /translation="MQRKGNKLLIQLCSVILLFFTTSWYALANECYIERNAEGDYHMK
                     ISSTQLSLASQMVEVPTEIAEATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTTNGAALLKTTVPGIVYSVELLCLSCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDQSWYLRFRLFITPEFKPKNGVSSGTTIAGKIASWYIGTNDQPWINFYIDNDSLK
                     FFVDEPTCATVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKIT
                     VKLKAANKPSDATLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNTVDYNFAAWSNN
                     LLFLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            72684..73478
                     /gene="panB"
                     /locus_tag="ACHJ48_000280"
     CDS             72684..73478
                     /gene="panB"
                     /locus_tag="ACHJ48_000280"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO4705915.1"
                     /translation="MKPTTISSLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHESTLPVTVEDIAYHTTAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            73490..74341
                     /gene="panC"
                     /locus_tag="ACHJ48_000281"
     CDS             73490..74341
                     /gene="panC"
                     /locus_tag="ACHJ48_000281"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO4705916.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(74640..75536)
                     /gene="rpnC"
                     /locus_tag="ACHJ48_000282"
     CDS             complement(74640..75536)
                     /gene="rpnC"
                     /locus_tag="ACHJ48_000282"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO4705917.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            75810..76190
                     /gene="panD"
                     /locus_tag="ACHJ48_000283"
     CDS             75810..76190
                     /gene="panD"
                     /locus_tag="ACHJ48_000283"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO4705918.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(76194..77423)
                     /gene="yadE"
                     /locus_tag="ACHJ48_000284"
     CDS             complement(76194..77423)
                     /gene="yadE"
                     /locus_tag="ACHJ48_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4705919.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFDKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(77487..77927)
                     /gene="yadI"
                     /locus_tag="ACHJ48_000285"
     CDS             complement(77487..77927)
                     /gene="yadI"
                     /locus_tag="ACHJ48_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4705920.1"
                     /translation="MLGWVITCHDDRAQEILDVLEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSIWHQQQKNPPFVLKHNLYEY"
     gene            complement(78032..78802)
                     /gene="yadH"
                     /locus_tag="ACHJ48_000286"
     CDS             complement(78032..78802)
                     /gene="yadH"
                     /locus_tag="ACHJ48_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4705921.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(78799..79725)
                     /gene="yadG"
                     /locus_tag="ACHJ48_000287"
     CDS             complement(78799..79725)
                     /gene="yadG"
                     /locus_tag="ACHJ48_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4705922.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            79834..80496
                     /gene="can"
                     /locus_tag="ACHJ48_000288"
     CDS             79834..80496
                     /gene="can"
                     /locus_tag="ACHJ48_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO4705923.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLDEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(80537..81073)
                     /gene="hpt"
                     /locus_tag="ACHJ48_000289"
     CDS             complement(80537..81073)
                     /gene="hpt"
                     /locus_tag="ACHJ48_000289"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO4705924.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            81279..83669
                     /gene="gcd"
                     /locus_tag="ACHJ48_000290"
     CDS             81279..83669
                     /gene="gcd"
                     /locus_tag="ACHJ48_000290"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO4705925.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(83871..85421)
                     /gene="cueO"
                     /locus_tag="ACHJ48_000291"
     CDS             complement(83871..85421)
                     /gene="cueO"
                     /locus_tag="ACHJ48_000291"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO4705926.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKERAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            85587..85934
                     /gene="yacC"
                     /locus_tag="ACHJ48_000292"
     CDS             85587..85934
                     /gene="yacC"
                     /locus_tag="ACHJ48_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO4705927.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            86040..86906
                     /gene="speE"
                     /locus_tag="ACHJ48_000293"
     CDS             86040..86906
                     /gene="speE"
                     /locus_tag="ACHJ48_000293"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO4705928.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            86922..87716
                     /gene="speD"
                     /locus_tag="ACHJ48_000294"
     CDS             86922..87716
                     /gene="speD"
                     /locus_tag="ACHJ48_000294"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO4705929.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(87754..88116)
                     /gene="yacL"
                     /locus_tag="ACHJ48_000295"
     CDS             complement(87754..88116)
                     /gene="yacL"
                     /locus_tag="ACHJ48_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO4705930.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(88292..90889)
                     /gene="acnB"
                     /locus_tag="ACHJ48_000296"
     CDS             complement(88292..90889)
                     /gene="acnB"
                     /locus_tag="ACHJ48_000296"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO4705931.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            91244..93097
                     /gene="yacH"
                     /locus_tag="ACHJ48_000297"
     CDS             91244..93097
                     /gene="yacH"
                     /locus_tag="ACHJ48_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO4705932.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKTVPVTQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPGNPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(93168..94592)
                     /gene="lpdA"
                     /locus_tag="ACHJ48_000298"
     CDS             complement(93168..94592)
                     /gene="lpdA"
                     /locus_tag="ACHJ48_000298"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO4705933.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(94800..96692)
                     /gene="aceF"
                     /locus_tag="ACHJ48_000299"
     CDS             complement(94800..96692)
                     /gene="aceF"
                     /locus_tag="ACHJ48_000299"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO4705934.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAVPEA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNTGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(96707..99370)
                     /gene="aceE"
                     /locus_tag="ACHJ48_000300"
     CDS             complement(96707..99370)
                     /gene="aceE"
                     /locus_tag="ACHJ48_000300"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO4705935.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(99531..100295)
                     /gene="pdhR"
                     /locus_tag="ACHJ48_000301"
     CDS             complement(99531..100295)
                     /gene="pdhR"
                     /locus_tag="ACHJ48_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO4705936.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT        24349 a        27313 c        24836 g        23988 t
ORIGIN      
        1 attttttctc ttcaaccgaa ccggctgttt gtgtgaagtg attcacattc gccgtgtcga
       61 tggaggcgca ttatagggag tcggctcagg aagacaagcg gaaaaatgca tttttatttc
      121 aaccgctcat cttttaatca ttgcgccggt ttttgctgct tttttatcgc ttgcggcagg
      181 tctgccaggc tatttaacac ccaatctgcc gcgttttctg cttcaggtgt aataggttta
      241 cccgtacgca ccagcacttt tgttcccacg ttcgccgcaa ccgctgcctg catatcttct
      301 aatttatcgc ccaccatata agaagcggcc atatcaatat gcaaataatc gcgtgctgac
      361 aaaagcatcc ccggatgtgg tttgcggcaa tcgcagacct ggcgaaactc ttcaacacta
      421 ccctgcggat gatgcgggca ataatagata ccatccagat cgacatctcg gtccgccagc
      481 gaccagtcca tccactcggt cagcgtttca aactgtgctt cggtaaattt accgcgagca
      541 atgccagact ggttggtgac taccaccagc gcaaagccca ttttttttag ctcgcgcatg
      601 gcgtcaataa caccgtcgat aaattcaaag ttgtcgatct catggacata gccgtgatcg
      661 acattaatgg tgccatcacg gtcaagaaaa attgcgggta cgctcttcgc caccttttat
      721 agctccttaa taaggcatgt gacgctagta tcgcatgttt cgacctgcaa gaaagtgctc
      781 ttcgcataaa cctgattgat ttagacgtct ggatgcctta acatccattt cattgacggc
      841 gttgcccgtt tcaggcattc gagatgccac gactaactta atgacgataa taaataatca
      901 atgataaaac tttcgaatat caccaaagtg ttccaccagg gcacccgcac catccaggcg
      961 ttgaacaacg tcagcctgca tgtgccagct ggacaaattt atggcgttat cggtgcctca
     1021 ggcgcgggta agagtacgct tatacgttgt gtaaacctgc tggagcgccc aaccgagggt
     1081 agcgtgctgg tcgatggaca ggaactgacc acgctgtcag aatccgagtt gaccaaagct
     1141 cgccgccaga ttggtatgat tttccagcat tttaacctgc tctcttcgcg tactgttttt
     1201 ggcaacgtgg ctctgccgct ggagctggac aatacaccga aagacgagat caaacgtcgc
     1261 gtgacggaat tgctgtcatt ggttggtctt ggagataagc atgatagcta cccgtcgaat
     1321 ctttccggtg ggcagaaaca acgtgtggcg attgcccgtg cattagccag caatcccaaa
     1381 gtattgctgt gtgatgaagc caccagcgcg ctagacccgg caacgacacg ttctattctc
     1441 gaactgctga aagacattaa ccgccgtctg ggtttgacga ttctgttgat cacccacgaa
     1501 atggacgttg tgaagcgtat ttgtgattgc gttgcggtca tcagcaatgg cgaactgatc
     1561 gagcaggaca cggtaagtga agtgttctca catccgaaaa cgccgctggc gcagaagttt
     1621 attcagtcaa ccctgcatct ggatatcccg gaagattacc aggaacgtct gcaagcggag
     1681 ccatttactg actgcgtgcc gatgctgcgt ctggagttta ccggtcaatc ggtcgatgcc
     1741 ccactgcttt ctgaaaccgc gcgtcgtttc aacgtcaaca acaacattat tagcgcgcag
     1801 atggattacg ccggtggcgt taagttcggc atcatgctga ctgaaatgca cggcacacaa
     1861 caagatacgc aagccgccat tgcctggctg caggaacacc atgtaaaagt agaggtactg
     1921 ggttatgtct gagccgatga tgtggctgct ggttcgtggc gtatgggaaa cgctggcaat
     1981 gaccttcgta tccggatttt ttgggtttgt ggttggtctg ccggttggcg tcctgcttta
     2041 tgtcacgcgt ccggggcaaa ttattgctaa cgcgaagctg tatcgtaccg tttctgcgat
     2101 tgtgaacatt ttccgttcca tcccgttcat tatcttgctg gtatggatga ttccgtttac
     2161 ccgcgttatt gtcggtacat cgattggttt gcaggcagca attgttccgt taaccgttgg
     2221 tgcagcaccg tttattgccc gtatggtcga gaacgctctg ctggagatcc caaccgggtt
     2281 aattgaagct tcccgcgcaa tgggtgccac gccaatgcag atcgtccgta aagtgctgtt
     2341 accggaagcg ttgccgggtc tggtgaatgc ggcaactatc accctgatta ccctggtcgg
     2401 ttattccgcg atgggtggtg cagtcggtgc cggtggttta ggtcagattg gctatcagta
     2461 tggctacatc ggctataacg cgacggtgat gaatacggta ctggtattgc tggtcattct
     2521 ggtttattta attcagttcg caggcgaccg catcgtccgg gctgtcactc gcaagtaacg
     2581 ttcaacacaa cataaataat tgaagaagga ataaggtatg gcgttcaaat tcaaaacctt
     2641 tgcggcagtg ggagccctga ttggatcact ggcactggta ggctgcggtc aggatgaaaa
     2701 agatccaaac cacattaaag tcggcgtgat tgttggtgcc gaacagcagg ttgcagaagt
     2761 cgcgcagaaa gttgcgaaag acaaatatgg cctggacgtt gagctggtaa ccttcaacga
     2821 ctatgttctg ccaaacgaag cattgagcaa aggcgatatc gacgccaacg ccttccagca
     2881 taaaccgtac cttgatcagc aactgaaaga tcgtggctac aaactggtcg cagtaggcaa
     2941 cacttttgtt tatccgattg ctggttactc caagaaaatc aaatcactgg atgaactgca
     3001 ggatggttcg caggttgccg tgccaaacga tccaactaac cttggtcgtt cactgctgct
     3061 gctgcaaaaa gtgggcttga tcaaactgaa agatggcgtt ggcctgctgc cgaccgttct
     3121 tgatgttgtt gagaacccaa aaaatctgaa aattgttgaa ctggaagcac cgcaactgcc
     3181 gcgttctctg gacgacgcgc aaatcgctct ggcagttatt aacaccacct atgccagcca
     3241 gattggcctg actccagcga aaaacggtat ctttgtcgaa gataaagact ccccgtacgt
     3301 aaacctgatc gtaacgcgtg aagataacaa agacgccgag aacgtgaaga aattcgtcca
     3361 ggcttatcag tctgacgaag tttacgaagc agcaaacaaa gtgtttaacg gcggcgctgt
     3421 taaaggctgg taatttttag gctgtttcca caatttgtaa tatcattcag gacgggcgct
     3481 tgcccgtctt gtcattttta caagctcctg attcaatatt gacgttttga tcatacattg
     3541 aggaaatact atgcgtgctt taccgatctg tttagtagca ctcatgctaa gcggctgttc
     3601 catgttaagc agatcccctg tcgaacccgt tcaaagcact gcaccccagc cgaaagcgga
     3661 gcctgcaaaa ccgaaagcgc cgcgcgccac gccggtccga atttatacca atgcagaaga
     3721 attagtcggc aaaccgttcc gcgatctcgg tgaagtcagt ggcgactctt gccaggcctc
     3781 taatcaggac tctccgccga gcattccaac cgcgcgtaag cggatgcaaa tcaacgcctc
     3841 gaaaatgaaa gccaatgcgg tattactgca tagctgcgaa gttaccagcg gtacgccagg
     3901 ctgctatcgt caggctttat gtatcggttc tgcgcttaac attacggcga aatgagcagt
     3961 ttccagttcg agcaaatagg tgttattcgc tcgccctata aagaaaaatt cgccgttccg
     4021 cgccagccag gtctggtaaa aagcgccaac ggcgaactac atctcattgc tccctacaac
     4081 caggctgacg ccgttcgcgg cctggaagca ttcagccatt tatggatcct tttcgtcttt
     4141 catcaaacga tggaaggcgg ctggcgtccg actgtgcgtc cgcctcgtct tggcggtaat
     4201 gccagaatgg gtgttttcgc tacgcgctcc actttccgcc ccaacccgat tggtatgtcg
     4261 ctggtagagc tgaaagaggt tgtttgccat aaagactgcg tgattctgaa gctcggcagc
     4321 ctggatctgg tcgatggtac gccggtagtg gatatcaaac cgtatctccc ctttgccgaa
     4381 tcgcttcccg atgccagtgc cagctatgcg caaagtgcgc cagctgcaga gatggcggtg
     4441 agttttaccg cagaggtcga aaagcagctt ttgacgctgg agaagcgtta tccgcagtta
     4501 acgctgttta tccgcgaagt actggcgcag gacccgcgcc cggcctatcg taaaggtgag
     4561 gaaacgggca aaacctatgc cgtctggcta catgatttta acgttcgctg gcgcgtcacc
     4621 gacgaaggtt ttgaagtctt tgcgctggaa ccgcgttaaa ttcacgccct tctcttttga
     4681 catttctttt gcactggtaa actaaatcac tttttttgtc ccaggctcgc cttgagcctg
     4741 ttctaccttc caactggaac cgtaacaaca tgcgtactag ccaatacctg ctctccactc
     4801 tcaaggagac acctgccgac gccgaggtga tcagccatca gctgatgctg cgcgccggga
     4861 tgatccgcaa gctggcctcc gggttatata cctggctgcc gaccggcgtg cgcgttctga
     4921 aaaaagtcga aaacatcgtg cgtgaagaga tgaacaacgc cggtgcaatc gaggtgtcga
     4981 tgccggtggt tcagccagcc gatttgtggc aagagagtgg tcgttgggaa cagtacggtc
     5041 cggaactgct gcgttttgtt gaccgtggcg agcgtccgtt cgtactcggc ccaactcatg
     5101 aagaagttat cactgacctg attcgtaacg agcttagctc ttacaaacag ctgccgctga
     5161 acttctatca gatccagacc aagttccgcg acgaagtgcg tccgcgtttc ggcgtcatgc
     5221 gttcccgcga attcctgatg aaagatgctt actctttcca tacttctcag gaatccctgc
     5281 aggaaaccta cgatgcaatg tatgcggcct acagcaaaat cttcagccgc atggggctgg
     5341 atttccgcgc cgtacaggcc gacaccggtt ctatcggcgg cagcgcctct cacgaattcc
     5401 aggtgctggc gcagagcggt gaagacgatg tggtcttctc cgacacctct gactatgcag
     5461 cgaacattga gctggcagaa gctatcgcgc cgaaagaacc gcgcgctgct gctacccagg
     5521 aaatgacgct ggttgatacg ccgaacgcga aaaccatcgc ggaactggtt gaacagttca
     5581 atctgccgat taagaaaacg gttaagactc tgctggttaa agcggttgaa ggcagtagct
     5641 tcccgctggt tgcgctgctg gtgcgcggtg atcacgagct gaacgaagtt aaagcagaaa
     5701 aactgccgca ggttgccagc ccgctgactt tcgcgaccga agaagaaatt cgtgccgtgg
     5761 ttaaagccgg tccgggttca ctgggtccgg taaacatgcc gattccggtg gtgattgacc
     5821 gtaccgttgc ggcgatgagt gatttcactg ctggtgctaa catcgatggt aaacactact
     5881 tcggcatcaa ctgggatcgc gatgtcgcta ccccggaagt tgcggatatc cgtaacgtgg
     5941 tggctggcga tccaagcccg gatggccagg gtacgctgct gatcaaacgt ggtatcgaag
     6001 ttggtcacat cttccagctg ggtaccaagt actccgaagc actgaaagcc tccgtacagg
     6061 gtgaagatgg ccgtaaccaa atcctgacta tgggttgcta cggtatcggg gtaacgcgcg
     6121 tagtggctgc ggcgattgag cagaactacg acgagcgcgg catcgtatgg cctgacgcta
     6181 tcgcgccgtt ccaggtggca attctgccga tgaacatgca caaatccttc cgcgtacaag
     6241 agcttgctga gaaactgtac agcgaactgc gtgcacaagg tatcgaagtg ctgctggatg
     6301 accgaaaaga acgtccgggc gtgatgtttg ccgatatgga actgatcggt attccgcaca
     6361 ctattgtgct gggcgaccgt aacctcgaca acgacgatat cgaatataaa tatcgtcgca
     6421 acggcgagaa acagttaatt aagactggtg acatcgtcga atatctggtg aaacagatta
     6481 aaggctgatg ccagaaaggg tcctgaattt cagggccctt ttttacatgg attgtataaa
     6541 atggataaac caaaggcgta ctgccgcctg cttctcccct gctttctttt actttcggcc
     6601 tgtaccgttg atatcagcca gccagactca tcagccacag cagtagatgc cgaggcgaaa
     6661 acatgggctg tcaaattcca gcatcaaagc tctttcaccg aacaatcaat aaaagaaata
     6721 acggtacctg atctcaaacc cggcgatttg ctgttctcct caagcctgga ggtaacctca
     6781 tttgggatcc gcgttttcag cacttcctct gtgagtcacg ttgcaatcta tctgggtgaa
     6841 aataacgttg cagaagcgac aggcgctggc gtccagattg tttcccttaa aaaagcgatg
     6901 aagcatagtg ataaactttt cgtcttacga gtcccggatc ttaccccgca acaagccaca
     6961 gaaatcaccg cgttcgccaa taaaatcaaa gatagcggtt ataactatcg cggcattgtc
     7021 gaatttattc cctttatggt gactcgccag atgtgctcac tgaatccgtt ctccgaggat
     7081 tttcgccaac agtgcgtcag cggcctggcg aaagcgcagt taagcagtgt ggtcgaagga
     7141 gacaaaaagt cgtggttttg ttcggaattt gtcactgatg catttgccaa agccgggcat
     7201 ccgcttacgc tggcgcaatc gggctggatt agccctgccg atctgatgca tatgcgtatt
     7261 ggtgatgtct ctgcgtttaa gccagaaacg cagttacagt atgttgggca cctgaagcca
     7321 gggatttata tcaaggcggg tcgctttgtt ggtttgaccc ggtgataaag taaatgcctt
     7381 atccgcctta aggtatgagg ggggttgtag gcatgataag acgcgccagc gtcgcatcag
     7441 gcgttgagca ccattgccgg atgcggcgta aacgccttat ccggcctaca aaaaacgtgc
     7501 aaattcaaaa tgttgaaata tatccttctg gcctgttttg cgtttgtttc tgtctcaaga
     7561 cgggttactg ccccaaacta ctgcaatcct ggtttgggta aaatttccct gccgtatcgg
     7621 gtgctaatac tttggttggc gcaccggtat ccggattagc ctcaagcgta aagtgacctt
     7681 ctactgacag taacaccggt ttttcactgt ggccgcgcgc agccaggtag ccacgctcca
     7741 gctctgcgtt attcgctacc atgaaacgtt ttccggtcgc gcaatcagtg aaggtcgccg
     7801 catcagccat ataaaaatac atgccccgca gggtcatcgg cgtcatgggt aaactggatt
     7861 gtgccggttc cagcgtatag ttgaactgcg attcaatcgg attgccttca cgatcgagca
     7921 tctccagcgc atcgcctttc gcccgataat atgacttttc acctttgctg tcggttaata
     7981 ccagcttgtc agcggttcgc gcccatgtac cgtaggaagc gaaggaggaa ggttcttcac
     8041 gagcaccgag ataacgctca ttcatcaccc atgttccgtc tttttcgagg aacagagagg
     8101 tttcgattcc ttcgcaatcg gcacacggca gcacgccgcg ccaactttgc ggcatcggtt
     8161 tcaattcggc agcctgcgcc ggagaaagcg tatcgacttc ggcccgatta ttacaaccca
     8221 tcagagtaaa gaggctgatt acagccatcg ctgtcactat cgcttttttc accatccatt
     8281 ccttcttttt attcccgacc gctgcgcatc gccttcacgc ttgatttttg tgctttcgat
     8341 gccagcctgc gctctttcga tgcacgggtg ggccgcgtgg gtcgtcgggc tttttgttct
     8401 gttgttaaat ctttaatcac tgccaccagc cgggccagcg ccgcttcgcg gttcagttcc
     8461 tgactgcggt attcctgtgc cttaatgaca attacgccat cactgctgat caaatgatgg
     8521 ctggcggcga gcagacgctc tttgtaatac tctggcaggc tggatgcccg aatgtcaaaa
     8581 cgcagatgaa tggccgttga ggtcttatta acatgctgcc cgcccgcgcc ctgcgcacga
     8641 atggcggtga tctcaagctc accatcggga atagcaacat gtcgggaaat cacaatcatg
     8701 aaggttgttg ccaggcggtt aagttcactt ccagattatt cttatcatcc gataaccaga
     8761 tcacgccatc ctgaatcgtt gcctgcagcg tcatggtacg atcggcaaag gcgctcactt
     8821 tcgccagctg ctcgtcgtcc agataccaga cggaaagatt ggcaaactgc gcacatttgc
     8881 tctgattttg ctgccaccag atttgcgccg cccgactatt ataggtaaac agcgccactt
     8941 ctgcggcctg ggtgcaggct ttcttaattc gtcgctcatc cggcagcccc agctcaatcc
     9001 acaaatcaat gcccaggtga tcgttacgca gccacgcttc cggctcatca tcggcacaca
     9061 aaccacgggt aaattgcaga cgttcatcag catatttcag ccacgccagc aggcgcagca
     9121 tcatacgctc ctgggtttct gaagggtggc gcgccagcgt cagagaggca tcgagaaact
     9181 ggttgcggtc gagatcggcc acattaaccg tcgctttata aattgtcgct ttaagcgcca
     9241 taagaaaact cctttcgaat caggaggcca ttgtaacgga atacgtgcag ttgcggcgct
     9301 gatattgcag aaatgagtgt ggtatagtca ccttgcgaaa cagagtttat cttcgtaggc
     9361 ttagacttgc atccacggtt aagtcagagt gctgacagga gggcatgtgg aaaaatattg
     9421 tgagttaata cgcaagcggt acgcggaaat cgccagcgga gacttaggat acgttccgga
     9481 cgcgctgggc tgcgttttga aagtgctgaa tgaaatggct gccgacgacg ccctttctga
     9541 ggctgtcagg gaaaaagcag catacgctgc ggcaaactta ctggtgagcg attatgtcaa
     9601 tgaatgatac gtatcaacca atcaattgtg atgattacga taatcttgag ctcgcctgcc
     9661 agcatcattt aatgctgaca cttgagctga aagatggcga aaaattgcag gccaaagcca
     9721 gcgatttagt ctcccgcaaa aatgtggagt atctggtcgt cgaggccgct ggcgaaaccc
     9781 gtgagctacg tctggataaa attaccagct ttagccaccc ggaaatcggt acggtggtgg
     9841 tgagcgagtc ctgattgact ttcgccggat gcgacgctta atgcgtcata tccggccttc
     9901 actaaactaa gcgtttcgct gccagacaaa acttatgcca ttctcacctt cagccacacc
     9961 ttcttgcgtc acaaatcccc ctgccgccgc aatcagcgtt tcgccataaa acagcagtgg
    10021 cgtggtgtca cgtagccacg gcggcacgcc cagctcttgc cagattttct ttagcttacg
    10081 tccgccgtta cgcccgacaa tatgcagcaa tcctggcgct ttgaaacgca cgctgaccgc
    10141 ttcgtctgca cgcggagggc gaatatcgcc tcccgcatta agctgtacac ttccctgccc
    10201 cgccggtaat tccagcggtt gaagccatgt ctgccacggc acaatgtttt cgctttgccc
    10261 ggtgacggat ttaatccacc acagttgcga ctgatagcgt cggatttcaa acgcgcccaa
    10321 acgtaaacag ggggaggcat cttcccgcgc cagcgccact tcctgccaga tcctcaccaa
    10381 cgcgtcgcgg gaaggcatcg gtgcattctg ccctgccagc cagcggcgga taatcgccgc
    10441 gcggcgggca tcactcatcg ccagcattgg cacaatctgc agcgtcccct gcggcgattg
    10501 acagtgtgct aaatcatctg ccagcagttc atccagcagg ctctcttgtt cagcacaaag
    10561 tgcggcgctg cgggccgttg tttcggcaaa atgcggccaa cgctgctgca ataacggcac
    10621 tacgcgcagg cgcagaaagt tacgatcgta tgagtcgtcc tgattacttt cgtcttcaat
    10681 ccagcgtaaa ccatgcgcca atgcccactg cgccagttcc ccccgcgtgc gggcaagcaa
    10741 cgggcggata agccgtgttc cggcaaactc cgaaacttcc gccatagccg aaagcccggc
    10801 agggccgcta ccgcgtttta gcgccagcag aaaagtttca cattgatcgt cgagatgttg
    10861 cgcggtgacc agcacttcac cgggcaacaa ggtgcgggca aatgcctgat aacgcgcctg
    10921 ccgcgcctgg gcctcaatcc ccagcccctc ttgcgcaagt tgtacgcgtt cgaccaccag
    10981 cggcacctgc cactgttggc agacgttttc gcaatgcgta acccaggcat cggcattggc
    11041 acttaaaccg tgatgcacat gaatagcgcg cagagcgccc cccggatttt ccgtccgcca
    11101 ctgcaccaac tgatgcagca gaacggtgga gtcaagcccg ccgctaaagg ccaccagaat
    11161 ctggcgtgag gtgagaagtt gtctattgag cgtgagtgtc atgatggtgc gattttacag
    11221 tagcaatgcc cggcacgtta ccgggcaaat gcggcgatga caagccttac tgctcataca
    11281 gttccaacgg cagcccgtcc ggatcgttaa agaaggtgaa gcgtttttgc gtgtatggat
    11341 cgacacggat ggcttcacac ttcacgttat ggctttcaag gtgcgccact gccgcatcga
    11401 tgtcatcaac gctaaaagcc agatgacgca ggccgcaagc ttccggtcgg ctggggcgct
    11461 ccggcgggaa cggaaatgag aaaagctcaa tcacatattg cccattaagc gccaaatccc
    11521 ctttccacga gtcgcgcgct tcgcgataga cttcgctttg cagcgtgaaa ccaagaatat
    11581 cgcagtagaa agctttgctc accgcataat ccgtcgcaat aatcgcaata tggtgaacct
    11641 gttttaaacc cagcataacg tctcctttat ttgttaacag cacgttactc gcccggaagc
    11701 cgctctggca agttatcccg ccatttttag gactcgtacg cggtaaacgc cgtcttcgtc
    11761 ctgtttcgca ccgtgaatat ccgtttcaaa accggggtaa tgttgcccta cggaacaaag
    11821 catcagtaga aaatcgagta ctgtgcggct ctctttggtc agcatttctc caggcatcaa
    11881 cagcggtacg cccggtggat aaggcaggat catatttgcc gatactctac cgaccagttg
    11941 ttccagcgca atggtttcta cttcaccttt aatctgtctc tgccatgcct gatgtggcgt
    12001 catgatcatc tccggcaaag tatcgaatgc ccgcaacatc aaaccgggaa gatcgtgttt
    12061 acgaatcagc ttatggatcc cttgcgccag atcctgaata cgcatattgc ggtagaaatc
    12121 gggatcttct gcgtacagat ccggaagcat atttttgatt cgcagattga gatcgtaaga
    12181 gcgtttaaat tccgtcaacc cacgcaataa tcccattgct ttggttttat cgatgccaat
    12241 actaaagaga aacagcaggt tatatgggcc ggttttctct accacgatcc cacgctcatc
    12301 gaggaacttc gccaccagcg ccgccgggat cccctcctcg ctcatattgc cctgctcgtc
    12361 catccccggc gtcagaatag tgactttaac cggatcgagg aacatatgat cggcatccgc
    12421 atcgttaaag ccgtgccatt gttcgccagg cgcaacgggc cagcattcgg cttcatccac
    12481 ctgcggcggt tgccagatat cgaaaaacca accgtcagac tcttcccgca gccgctggac
    12541 ctctttgcga aaatgcagag ctcgttctac tgaacggtta atcagccgtt tgcccggatt
    12601 accacgcagc atcgctgctg ccgtctcaac cgaagcaaca atgggataac tgggcgaggt
    12661 ggtggtatgc atcataaagg cttcgttaaa cgcctcttcg tcatactcac ctttaatgtg
    12721 gatcagcgaa gcctgcgata acgccgccag cattttgtgg gtcgattgcg tttcgaagat
    12781 cacttttccc gcaacacgct cgccgctcat accacttttc ccctggtaga tcggatgaaa
    12841 atgggtgtac ggcacccagg cggaatcgaa gtggatcgac gggacatcca gcgtctgttt
    12901 gatccagtcg gtgttgtaga gcaagccatc ataggttgag ttggtaatca ccgcatgaac
    12961 cggccattgt gcctgcgtgg tggcagcgac tttctcttcg atgctgtcgc gagtaaattc
    13021 acggcgcggg atcccaccca gaatccccaa cgcattacgc gtcggtttca gccagactgg
    13081 cactacatcg ttcatcatca acagatgcgc cagcgattta tgacaattgc ggtcaatcaa
    13141 cagcgtactg ccggatggcg cggcgtacat acccacaatt ttgttcgacg tcgatgttcc
    13201 gttggtaacg atataactct gttccgcgcc gaaagtccgc gcgatgtact cttccgcttc
    13261 caggtgtggc ccggtgtggt cgagcaacga accaagctcg gtgaccgaaa tagagacatc
    13321 agccttaagg gtattcccgc cgaaaaagtc ataaaacaga cagccaaccg ggcttttttg
    13381 atatgcggtg ccgcccatat gacccggcgt acaaaaggtg tacttccgct ctttgacgta
    13441 ggtaaacaag gctttcgtga acggtggggt aatgttatca agatattcgt cggtgtactg
    13501 acgcatacga atggcgatat cttccgcctg ccccagcgca tattcaaaaa aacagagcgc
    13561 catccgcata tcctgcacgc tgacatccat cgtcgagtgg gtgttgatga aggcataaag
    13621 cgggagatat tcattaagct gattgatatc gctacataaa tcgagactgt attcatccca
    13681 gtcaaaaatc acgccgcaaa ttcgagggtt atgttcgata aatttcagca aatcaacgct
    13741 gttttgtggc cagataatct gaaagccttg cgccaccagc gccgactcca gttctttgat
    13801 gggctcatct ttataaaaga cgccatgcgg tcccataatg gcaatgatgt tcatgtgttc
    13861 ctcctggaaa atccttcctt aatcatagcc tgctcaaacc gtggcgataa aaaagggcca
    13921 ccgaagtgac cctttttcag aacttttgcg aattacgcgt aaccgtagct catcaggcgc
    13981 tgataacgac gattttttaa atcttcagtg cttaacacgt cgagatcggc cagatccgcc
    14041 agcagttgcg ctttcaacga tgccgccatc gcttccgggt tacggtgagc gccgcccagc
    14101 ggttccggga tgatggagtc gatcagtttc agctctttca gacgcggagc gatgataccc
    14161 atcgcttcag ccgccagcgg cgctttgtcg gcgctcttcc acagaatgga cgcacaacct
    14221 tccggcgaga taacggaata ggtgctgtat tgcagcatat tcactttatc gcccacgcca
    14281 atcgccagcg caccgccaga accaccttca ccgataaccg tacaaactac cggtacgccg
    14341 aggcgagaca tttcacgcag gttgcgtgca atggcttcag actgaccacg ttcttctgcg
    14401 cccacgcccg gataagcccc cggggtgtcg ataaaggtga tgataggcat cttaaagcgt
    14461 tcagccattt gcatcagacg cagtgctttg cggtaacctt ctggcgctgg cataccaaag
    14521 ttacggcgaa ttttttcttt ggtttcacga cctttttgat gaccaatgat catcaccgga
    14581 cgaccatcga gacgggcgat accaccgacg atagctttat cgtctgcata cgcgcggtcg
    14641 ccagccagtt cgtcaaattc atcaaatgcc aggcgaacgt aatccagggt ataaggacgc
    14701 tgtggatggc gtgccagttg cgcaatctgc catgcaccga gatcggcgaa gattttacgc
    14761 gtcagttcta cgcttttttc acgcagacga tgcacttctt catcgatgtt aatatccagt
    14821 ttctcatcct gacggctaac cgcagtcaga gaatcgattt tcgcttccag ctctgcaatc
    14881 ggctgttcaa aatcaaggaa attcagactc atagtattcc tgtattagtc aaactccagt
    14941 tccacctgct ccgaaccaat gaggccacgg agatcgttta ataaacgatc gctcggagag
    15001 acacgccacg tcgcgccaaa acgcaaccgc gcgcgtgcat ccgccctctg atagtagaga
    15061 tgtactggaa ttgtcccaga gcggtggggt tccagagact gacggagtcg gtttaaaagc
    15121 tggtcatcaa tttgcctgtc cgtcagcgag atagcaagcc cgcgagcata tttttcccgg
    15181 gcttcgtcaa tatccatcac ttcgcgagcg gtcattttaa gcccaccgct gaagtcatca
    15241 aagctgacct gtccgctgac gataagtatg cggtcttttt ccagcaaatg ctggtattta
    15301 tccagggcgt cagtaaacaa catcacttcc agccgcccgg aacggtcatc cagcgtgcag
    15361 ataccgatac gattgccgcg cttggtgacc ataacccgcg cggcaacaac gagccccgca
    15421 gccgtgatga ctttaccacg ttctgtcggg tgcatgtctt tcagccttac gcctccgaca
    15481 taacgctcaa tctcttttaa atactggttg atagggtgtc cggtcaggta caggcctaac
    15541 gtttcacgtt ccccatctaa taccacctgc tccggccacg gttggcagct ggcataggat
    15601 tgttcaattt gttccggctc ttcggccagc acgccgaaca tatcggcctg accgatagct
    15661 tccgctttcg cgtgttgatc tgccgctttt aacgcatcgc ccagcgagtt catcagcgcc
    15721 gcgcgatgtg gcccaagacg gtcaaacgcc ccggacatga tcagtttttc cagcacgcga
    15781 cggttcaact ttttggtgtc ggttcgggcg cagagatcaa acagttcgcg gaagtagccg
    15841 cctttattac gggcttcgat gatcgcctca atcggacctt cacctacccc tttgatcgcg
    15901 ccaataccat acacgatttc gccgtcgtcg ttgacgtgga aatggtaaag accggagttt
    15961 atatctggtg gcaggatttt cagccccatc cgccagcact catccaccag gcccaccacc
    16021 ttctcggtgt tgtccatatc ggcggtcatt accgccgcca taaactctgc cggatagtgc
    16081 gctttcaacc ataatgtttg atatgacacc aaagcatagg ccgcagagtg cgatttgtta
    16141 aatccgtaac cagcgaattt ctccaccagg tcgaagattt tcatcgccag ttcggcgttg
    16201 attccgttct tttctgcacc ttcagcaaat acagaacgtt gcttagccat ctcttccggc
    16261 tttttcttac ccatcgcacg acgcagcata tccgcgccac cgagggtata accagaaagc
    16321 acctgcgcaa tctgcatgac ctgttcctga tacaggataa tgccgtaggt tggctccagt
    16381 accggtttca ggctttcatg ctgccactgc acgtccggat aggagatctc ttcgcgacca
    16441 tgtttacggt cgataaagtt atccaccatc cctgattgca acggaccagg gcggaacagt
    16501 gccaccagtg cgatcatatc ttcgaagcag tcaggttgca gacgcttgat caggtccttc
    16561 atgccgcgcg attcaagctg gaataccgcc gtggtttccg agcgttgcag catgtcgaag
    16621 cttttcttgt catccagcgg gatcgcggcg atatccagcg gcggctcgcc attcttcgcc
    16681 cgccgcttgt tgatcatctc cagcgcccag ttgatgatgg tgagcgtacg caaaccaagg
    16741 aagtcgaact tcaccagtcc ggcgtattca acgtcgcttt tatcaaactg ggtgaccgga
    16801 tgtttgccct cttcatcgca ataaagcggc gcaaaatcgg taattttggt cggcgcgata
    16861 accaccccac cggcgtgctt accggcgtta cgggtgaccc cttccagttt gcgcgccatg
    16921 tcgatcaacg ccttaacttc ttcatccgct tcgtagattt ccggcagctg cggctcggct
    16981 tcaaacgctt tcgccagcgt catccccgga tcgggcggga tcagtttcga gatacgatcg
    17041 acaaagccgt acggatgccc cagcacgcgg cctacgtcgc ggatcaccgc tttcgccgcc
    17101 atcgtaccga aggtgataat ctgcgatacc gcatcacgac cgtacatgtc cgctacgtgt
    17161 tcgataacct gatcgcgttt ctccatacag aagtcaacgt cgaagtcagg catggagaca
    17221 cgttccgggt taaggaaacg ttcgaacagc aggtcaaatt ccagcggatc gaggtcggtg
    17281 attttcagcg cgtaggccac cagtgaaccc gcaccggagc cacggcctgg ccctaccggt
    17341 acgccgttat ctttcgacca ctggataaat tccataacga tgaggaagta gcccgggaag
    17401 cccatctggt tgataacctg aagttcagtc tccagacgtt cgtcatattc cgggcggcgc
    17461 ttaacacgtt cttcctcatc agggaataaa aaggccagac gctcttccag gccctctttt
    17521 gcacgcttga ccagataatc ttcggtgctc atgtccccgg tcgggaactg cggcaggaag
    17581 tattcaccaa gacgcacagt tacgttacag cgtttggcga tctcaacggt gttggcaagg
    17641 gcttctggga tgtcggcaaa cagctcgcac atctcctctt cgctacgcat atattgctgc
    17701 ggcgaatagt tacgcgggcg tttaggatcg tcgagggtaa agccgtcgtg gatcgcgacg
    17761 cggatttcgt gtgcgtcaaa gtcgctgctg tcgataaagc gcacatcgtt agtcgccacg
    17821 acgggcaaac cgcgcgcttc cgccagttcc accgccgcgt gcagatagct ttcttcatcc
    17881 ggcctgccgg tgcggatcag ctcgagaaaa tagcgatccg ggaagtgttc ttcataaaac
    17941 gcgacacact catctaccag cgcgctgtta ccacgcaaaa gactgcgtcc gacgtcgccc
    18001 atgcgcccgc cggaaagaag gatcaaccct tcgtttaatt cgataagcca gtcgcgatcg
    18061 atgatcggcc cggcggcacc gtacccgcgc tgatacgctt ttgagatcag caacgtcaga
    18121 ttctgatagc cggtattgtt cgccgccagt accgtcaggt gggttaactc atcacccagc
    18181 aggtcgcact ggacgttaaa atctgccccg acgataggct taatccctgc gccatgtccc
    18241 gctccgtaga acttcaccag accgcaaagg ttggtgaaat cggtgatcgc cagtgctggc
    18301 atacccaacg ccgccgcctt ttttaccagc ggtgcggttt tggccaggcc atcgatcatc
    18361 gagtagtcgc tgtgcacccg caggtgtacg aaacgtggtt cagacatctt cagattccgg
    18421 tttacttaat ctcgacacaa gaatcaggac gcaagtccca gtgcgcgttt gacaggccca
    18481 aagctgcgcc gatggtgttc ggtcgcgccg tgttcagcca gtttttccag atgaaaagcg
    18541 gttggatacc ctttgtgttg ggcaaaacca tattgcggga aaacaatatc cagcgccgcc
    18601 atttcggcgt cacgcgtcac tttcgccagg atagacgcgg cactgatttc cggtacgcgg
    18661 ctatcgcctt tcaccacagc catcgcaggc atcggtaatt tcgggcagcg gttaccatca
    18721 atcaacacat attccggcgc aatatgcagc ccagcgacgg ctcgctgcat cgccagcatg
    18781 gtcgcatgta aaatattcag ttcgtcgatt tcgtgaggtt ccgcgcggcc cagactccag
    18841 ctcaacgctt tctctttgat ctcttcacag agcgccagac gacgtttttc gctcagcttt
    18901 ttggaatcat tcagcccggc aatcgggcgc gccgggtcga gaatcaccgc agcggtgacg
    18961 accgcgccaa ctaacggccc gcgtccgact tcatccacac ccgcaaccag ctgcgtgtgc
    19021 ggatagacaa attcgatcat tgtgctaact ccagaacggc ttgtgccgcc tgctcatcgg
    19081 cattgcagcg gatctgctga tgcagttcac ggaaggtatc gtgcatcgcg tggctggttt
    19141 tcccattcgc caacagcggt aacagcgccg cagccagttt ttgcggctca cactcttcct
    19201 gcaataactc tttgactaac tctctgcccg ccagcagatt tggcagcgag acataatcag
    19261 ttttcaccag ccgcttcgcc aaccagaagg taaaaggctt catgcgatat cccaccacca
    19321 tcgggcattt cgccagcata cactccaggg ctgccgtccc cgacgccagt agtgccgcat
    19381 cgctggcgac catcgcctca cggcccattc catccagcaa atgaaccgac aggtctggcg
    19441 cgactgcagc tttgatgcgt tcaaactgct cgcggcgttt ggcattcacc agtggcacca
    19501 cgatttcgag atccggatat gtctggcgca aaagctgggc cgttttcagg aaatcggcac
    19561 taagcatttc gacttctgca ccacggctgc ccggcaacaa tgccagacag tgggcatcgt
    19621 aagggatccc cagcacatca cgggcactat ttttatctgg atctaatggc atggcatcag
    19681 ccatggtatg accgataaag cggcacggta cgttgtattt gtcataaaac gctttttcga
    19741 aaggcagaaa tgcgagcacc agatcggtgg ctctgcctat tttgaaaacg cgtttctgtc
    19801 gccacgccca gacggacgga ctgacataat gaatggtttt gataccctgc tttttaaggt
    19861 taccttcgag gataatattg aagtcaggcg catcaatgcc aacaaaaaca tctggcttta
    19921 gttcaccaaa acgctttgtc agatcggcac gaatatgcag taagcgacgc agacgaccga
    19981 gcacttcaac aatgcccatc accgccaact cttccatttc gtaccaggct tcgcagcctt
    20041 cagcctgcat tcgtggcccg gcaacaccaa caaagcgggc gttgggcaca cgttctttca
    20101 gagcgcggat taaaccggcc cccaggatat cgccggaggt ttctccggcg accagggcaa
    20161 tcgttaatgg acgctgttca gtcattaacg aatcagaccg cgcgttgagc gtgcaaagaa
    20221 atcggtaaag gctttcactt ccggatatgt ttccgccagt tcagcaattt ccggtttcac
    20281 ttcatcgagc gttttaccgc tacgataaat cagcttatac gcattgcgga tagcggtaat
    20341 cgcctcacgg ctgaatccgc ggcgcttcag cccttcgata ttgacaccga acggcgtggc
    20401 gtggttaccc tgcgcaatga cataaggagg gacgtcctgc gccacaccgg agcagccgcc
    20461 aaccatcaca tgcgcaccaa tgatgcagaa ctgatggact gcggccatgc cgccgatgat
    20521 cgcgaagtcg tcaaccgata cgtgacccgc cagcgttgcg ttgttggcga gaatacagcg
    20581 gttacctacc gtacaatcgt gcgcaatgtg cgcgttgatc atcagtaagt tgtcgctgcc
    20641 caccttcgtc aatccaccgc cctggactgt gccacgatga atggtgacgc tttcgcgaat
    20701 gcggttacga tcgccgattt ccacgcgggt cggttcgcca gcatatttca gatcctggtt
    20761 aacttcgccg atggaggcga actgataaat ctcattatcg cggccaattt tagtatgacc
    20821 attcacgaca acgtgagatt tcagtacggt accctcacca atttcgacat ggggtccaac
    20881 gatacaaaaa ggaccaatgt gtgcgttcgc gccaatcgac gcgccctctt ccacaatggc
    20941 ggttggatgc acaaaggcgg atttatcaat cacgtatcag gcctcccggc tacgagcaca
    21001 catcatcgtt gcttcgcaaa ctactttacc gtcgaccaga gcaacccctt taaaacgggt
    21061 caggccgcgg cgcgtttttt cgaaagtgac ttccatgatc atttgatcgc caggcacgac
    21121 cgggcgcttg aagcgcgctt cgtcaatacc agcgaagtag tacagctcac ccggttccag
    21181 ttttcctacg cttttaaacg ccagaatacc tgttgcctgt gccattgctt ccagaatcag
    21241 cacacccggg aaaatcggtt ttccagggaa atggccctgg aagaatggct cattgacaga
    21301 gacatttttt actgcgcgca gaaaacgacc ttcttcaaaa tccagcacgc gatccaccag
    21361 taagaacggg aaacggtgcg gcagaagttc taaaatctct tcaatctgca gagtatgagt
    21421 gttagtagtc aagatactct tcctgtcaaa atataagaaa cgacaataat aacacggcct
    21481 gccgcaatcg taagaatgag acaggccgta aagtttggcg aacaaaagat ggaacgttag
    21541 tcttgttgat taaccttgcg ctcaagcaat ttcagacgct tgctcatgtc atcaatgttc
    21601 atcaccagtg cagcggtttt gcgccagact ttgttgggtt gcagcggaat gcctgaggaa
    21661 tagacgcctg gttcagtgat gggacgcatc accataccca tgcccgtaac cgtcactttg
    21721 tcgcatattt ccatatgccc gttgattacg ctggctccgc cgatcatgca gtaacgacca
    21781 attttcaggc tgcccgccat aatgacgcca ccggcaaccg ccgtattgtc gccaatcacg
    21841 acgttatgtg caatctggca ctggttatca atgatcacgc cattgccaat aacagtgtca
    21901 tccagcgcgc cgcgatcgat ggtagtgcag gcaccgatct ccacgcgatc gccaataatt
    21961 acgcgaccaa tctgtgggat cttcacccag ttaccacgat cgttggcata accaaagccg
    22021 tctgcgccta ccactgttcc ggactggatc aggcaattct gaccgatctg gatctcatgg
    22081 taaatggtta cgttcgccca gagacgcgaa cctgcaccga ttttgctgtt tttacctacg
    22141 aagcaaccgg caccgataat cacgttatcg cccagttcaa cgccggattc aatcaccgcg
    22201 ttagcgccaa tcgatacgtt gttacccagc ttcgccgtcg cgtcgatcac cgcactgggt
    22261 gcaatgttct gcgcgggctg cggcgtggta tctaaaattt gcgccatgcg cgcgtaagtc
    22321 aggtagggat tcttcactac cagcgcggca cttttcgcga aaggaagatc gtcctgggtc
    22381 atgacaaccg cggacgcctg gcacaagcct aaatgctcac ggtattttgg gttaaccatg
    22441 aacgtaatgt gacctgtttg tgcagattgc atggacgcaa cgccggtgat gacgatatcg
    22501 ccatcaccgt gtagttctgc atccaactgc tgcgctaaat cagccagtcg aattgaaggc
    22561 attacttatt taacctgttt cagtacgtcg gcagtgatgt cttttacatc gctgctgttg
    22621 taagcaacgg cgtttgcatc aacaaccaga tcgatatcct ggctgttggc aacggatttc
    22681 acagcagtct ggatacgagt aaccagtttg ccgcgttctt cattggaacg acgtgcgcga
    22741 tcctgctcaa aagcctgcgc tttctgagca aaagtctggc gctgagccat cacgtctttt
    22801 tccagcttag tgcgatcgct gcccgctttc atggactgca gctttttcat tttagcctgc
    22861 agatcggttt ccatacgctg cagttcgctg gcacggcctt tgaactcatt ttccagcgtg
    22921 ttagaaacac cggttttctg cgctacctgc tggaacaggc tgcccatgtt gacgattgca
    22981 attttgtcag ccgcctgagc agatgttgcc agtgctaaac cgagacctgc agctaataac
    23041 cactttttca caataaactc cttaccatcc catttgcacc ggagggtgca gttctttgcg
    23101 tggcccggcg atttatattg atcgcctaaa gtcatcgcta cactaccact acattccttt
    23161 gtggagaaca cttaccaggt tttaccgatg ttaaactgga actgttctgc cttgtctcca
    23221 tcgtactttt tgaacggctg ggcgtaggag aacaccaacg gccccaatgg ggacatccat
    23281 tgtaatgcga tacccgcaga catacggata ttgcttggat cactatagtc cggatatcca
    23341 gaatattggc tggaatccca gtttgtatcc caaacggtac ccatatccca gaagaaggaa
    23401 gtacgaaccg agttagcata cttatcgcta ataaacggcg tcggggtgat gaactcgagg
    23461 ctggcaaccg ccatggcgtt accgcctaca gcatcatccg atttacacag gtctttcgcg
    23521 ccgtcctgag tcgcacattc gtaatcatag tccggatcat aattactggc ctgatgcggg
    23581 aagtaaactg ctttcggacc aattgtattg gactggaagc cacgcacggt gctggaacca
    23641 ccggcataga agttctcgta gaacggcatc tctttgccgc ctaaaccatc accataaccc
    23701 cagcgggtac gccccagaac aacccatttg tgatcgtcat cgatcggcac ataagtcgcc
    23761 gtgtctaacg tcactttgta gtattcgtta tccgatccag gaatggtcac tttaccggtc
    23821 aggttgacac gtgaaccatc tgtcgggaag taaccacggt caagcttgtt ataggtccaa
    23881 ccatagttga acgtgaagtc gtccgttttg aagctgttat cctgatcaga ggtgctcgga
    23941 tgttcaccca tagagtacag ataacgccac atcgcaacct gaggctgcat gttggacagg
    24001 gagttatgta cataacccag acctgcacgc agcgagttat attcgttaat cgggaagccc
    24061 aacgtcacgt ctgtaccata actcttgttg gtatagtcgg acaggtcggc gtcatctgcc
    24121 tggaagtcat tatagaagag acgaccaccg aggcttacgc catctacggt gaagtacggg
    24181 ttggttaccg acagttcagc ataggtctgg taatcgtttt tggtcccgtt gataccaaca
    24241 gcataacctg tacctaacca gttatcctgt tgcacgccag cctggaagct cacgccactt
    24301 tcagtaccgt aaccaatccc gaagttgaag ctaccagtgt tacgttcttt caccttgtag
    24361 actacatcta cctggtccgg gctacccgga acacgttggg tatcggtatc gacagtttca
    24421 aagaagccca gacgattcag acgctcctta ccctgatcga ccagatcgct tcccagccat
    24481 gcaccttcca tctgacgcat ttcgcgacgc aggacggcat ctttcgaggt atcgttacct
    24541 tcaaaacgga tcttacgcac gtagaaacgg ttacccgcat caacgttcac gcgtaattta
    24601 acggttttgt cggcatcgtt aatttcaggc atcgactgta cgcgcggata ggcataacca
    24661 tagcgaccga gaagcttttt aatgtcatct tccattttgg tcactttggt gccgttatac
    24721 agctcgcccg gctcgatctt agtcagctgc tcaatttcag cggagtgccc ggcaaggttg
    24781 ccgctcactt caacgccaga aagcttgtac tgatcgcctt cggtgatgtt caccgtgacg
    24841 taaatacctt ttttatctgg cgtcagactg acctgggtag agtctatgtt gaaacgggca
    24901 taaccgcggt ccagatagta gctgcgcagg gtttcaaggt cgcccgccag tttctgtttc
    24961 tggtatttac gatcgcctac cacgttccac cacggcactt cgtcacgcag ttggaaatga
    25021 gagatcagtt cgtcggtggt gaaagcatgg ttaccaacaa tgttaatttg ctggatttca
    25081 gctgacacac cttcctggaa caccagtttt aggtcaacac ggttgcgcgg cagcggggtc
    25141 acgacagctt taacgctggc gctatattta ccgacgctgt agtagaagtc ttccagacct
    25201 ttctcgatat cggcaatggt ggtgcgatcg agggattcgc ccacacgcac accagaagcc
    25261 tcgaggtttt gcttcagcat gtcatctttc accgatttgt taccggagaa agtaatgctg
    25321 gcaatggtcg gacgttcttt tacctgaacc agaagggtat caccatcacg aaggacgcga
    25381 acatcctcaa agttgccggt agcaaacaga gcgcgaatgg tattactgat atcttcatca
    25441 ttaaccgtgt cgcctgtgcg caccggcata ctgaggaggg ccgcaccaac ggcgacacgc
    25501 tgaaggcctt cgaaatgaat atctttcact acgaaccctt cagcaccgta tacggtggcg
    25561 ctgctaaaca gcagcgacgc tatgagcaac tttttcatcg ccatcgttat tatgcgttct
    25621 tcctaactaa ctctcataac cgagagaaat cattgaaaag tgcaagcccc attaacagca
    25681 ccagcagaat cgagccaatg cgataacaaa agtcttgaac ccgctcggat accggtccgc
    25741 ctttgatctt ttcgatcgca aggaacagca gatgcccccc gtcaagtacg ggcaacggaa
    25801 acaggttaat tatccctaag ttcacgctaa taagcgcaag aaacggcagg taataaacta
    25861 ccccgagttc cgctgtcatc ccagccccct tggcgataga gatcggccca ctgaggttgt
    25921 tcagtttcac atcaccggtg atcaattttc ccagcatact gaccgtcagc ttcatcagct
    25981 gccacgtttt gtccgtggct tcgacgatgg cgttgaacgg cccatactgg cgtacaactt
    26041 tatactcatc tggcaaagga atgactttcg gctcaatacc gacaaaacca atcgctttac
    26101 cattacccgg tttactctcc gggattaatg tcaaagacaa gggactcccc tgcctttcga
    26161 tttctaacgc taaggattta cccgggttat cccggacaag catcacaaag gtcacccact
    26221 gcgttaaggg ctgaccatcg actttaacga tcctgtcgcc tgcttgcaaa cctgccttgc
    26281 ttgccgccga gtttggctgc acattttcca gtacaggttc aatttgcggc ccacgaggac
    26341 gaatccccag cgaagatacc ggatcttctt tatcaggctc aaacgcccag tgacgtaaat
    26401 cgagctttac atcccgccgt tggtcgctgc caaatggcgc tactgtaatg gtggtgcttt
    26461 catcgccaat tttatcgacc aactgcaaac gcacggcatc ccaatcaggc gtttcgatac
    26521 catctacggc ttttagttcc gtacctggtg caatttgtgc ttccgcagct atcgaattgg
    26581 ctgctatttc gccaaccacc ggacgtacgc caggcacacc aataataaaa accagccagt
    26641 aggcaaagat agcaaaaatg aagtttgcaa ccggacctgc ggcaataatc gccgctcgtt
    26701 ggccgacaga tttattattg aaggcatggt ggcggagttc cggaacgacc ggttctgcgc
    26761 gctcatccag cattttgaca taaccgccca acgggatcag ggcgataaca tattcggtgc
    26821 cgagcttatc agttcgccgc cagagcgcct taccaaaccc tattgagaaa cgctcaacgc
    26881 gaacaccaca acgccgggca acccagaaat gaccaaattc atgcacggtg ataagtacac
    26941 ccagtgcaac gatgaacgaa gccaaatccc agagaaaact cagcatatta ccttccgtta
    27001 aagcgtcctg aataccagta acaacaagca agcaaagacc ggtaccgcag ccgtcaggct
    27061 atcaatacga tctaaaatac caccgtgtcc tggaattaaa tgaccgctgt ccttaattcc
    27121 tgcttcacgc ttaaacatac tctcggtcag atcgccgagc actgaggcta acgctgcgac
    27181 aatagagcaa atgagtaagg tgacgggagc gacgtcgaga ttcgcccaca tgccataacc
    27241 ccatgagatt accgctgcag tagcgagtcc accgataaag ccttgccagg ttttacccgg
    27301 agaaaccttc ggtgccagct tatgtttacc aaacaatttg ccaaacatat atgcgccgga
    27361 gtcagcgccc cataccagga tcatgacata gagcagccat attgcgccac tgtaatgatt
    27421 ctcgtcatag tgccaggccc gtaacgccag catgccccag aagaagggaa caatggttag
    27481 cacgccaaaa ataaggcgca atgttttaga gttacgccag attgctgcgg aacctgggta
    27541 aaacagcacc agcaatagcg cgacaatcca ccaacccagc gaagcccaaa gtgagatttc
    27601 aaccagcggt tgatgaatat ttcggtgata ttccggcaac agaaaaagca tcagcgccaa
    27661 caataacccg cataacaccg ccaaccatac tcgctgcgaa cgagtggtaa aaccgctaag
    27721 ctgtccccat tcccacgctg ccagcatgca gaccaccagc gttacaatgg cgaaccccac
    27781 cggcggcaac agaaacaacg ccgcgatgac gacgggtatt aacacaaaag cagatatcag
    27841 gcgatacttc agcaaaagcg acccccatca ggctgtttca tcaccgggct cggtgccgcc
    27901 gaaacgacgc tctcgattag caaaggcatt taacgcccct tcaaagtctt gttcatcgaa
    27961 atcgggccag agaacatctg taaagtaaag ttcggcatag gcaatttgcc aaagcaaaaa
    28021 gttactaatg cgatgctccc ccccagtcct aattactaaa tctacagggg ccagttcatg
    28081 catacagaca tgctggttta gcatctcttc atctatctga tctggttgca ggtttccttg
    28141 ctgcaccttt tcagccagtt gcctgactcc ctggactata tcccaacgtc caccgtagtt
    28201 cgccgcaata ttcagcgtca gaccggtatt cccggctgtt agcgcttcag atttacgaat
    28261 acgttcttgc aaacgcgagt taaagcgact ggtatcgcca ataatacgca gacgcacgtt
    28321 atgtcggtgc agacttttta cttcgctatc gagcgcccac acaaacagtt ccattaacgc
    28381 actgacttcc tgcgctggtc ggttccagtt ttcactacta aaggcataca gcgttaacgc
    28441 ctcaataccg ttgttggccg caaaagagac agcccggcgg acggattttg ctccggcttt
    28501 atgcccaaag gcacgaatct tcccttgctt ttttgcccag cgaccattgc cgtccataat
    28561 gatcgcaaca tgacggcagc catgcgctgg caatttttcg ctaagtggtt gagtagcaga
    28621 caacatcacg cgttttttat tccctgacag ggtttacggt actcaggaat actgaagcct
    28681 atacaaaaaa aagccgtgta aaaccacggc atacctgacc ttataaagcc aactacttac
    28741 gatcagatgg cgcagactat atcactgaag ccctacgcta acaaatagcg cgactctctg
    28801 tagccggatt atcctcagct tgcgagacgc atcacctctt ttctggcgac ttcacgcgcg
    28861 ctcgcatcaa cagataacac atcgtccaca cattgtggtt cgcgcatatc cattttttcc
    28921 agtacggata aattcaacgc agcgatatcc gtaaagcgga tttgttgcgc aagaaaagca
    28981 gcaacggtga tttcgtttgc ggcattcaat gctgtcgtcg ctgcctggcc ttgttcgaac
    29041 gcctccatcg ccagtttcag gcatggataa cgatcataat ccggtgcggc aaatgtcaac
    29101 gcacttagtt tgcaaaaatc gagcggcttc acgccagagt tcacgcgatt cggccatgcc
    29161 atggtgtggg caattggcgt acgcatatcc ggttccccca gctgcgccag aacactgccg
    29221 tcctgatagc gcaccattga gtgaatcact gactgcgggt gaatcagcac ttccatctgg
    29281 ctggcgctgg cgttaaacag ccaacgcgct tcaatgtatt ccagaccttt gttcatcatg
    29341 gtagccgaat cgacagaaat tttacgtccc atcgaccagt tcggatgacg gcaggcttga
    29401 tcgggcgtca ttgttgccaa atcgcgcaat ggcgtctcac ggaaagggcc accagacccg
    29461 gtaagtaaaa tggacaccac gccattttgc tcaaggtcag cgtatcccag attatgctgg
    29521 ataggttgcg gtaaactctg aaaaatggcg ttatgttcgc tatcgaccgg taacaattgc
    29581 gctttgctct gctttacggc gtccataaac agacgtccgc aggtaaccag tgattcttta
    29641 ttggccagca aaatggtttt acccgcgcgg atcgcagcaa gcgtaggtaa cagcccagca
    29701 gcgccaacaa tggctgccat cacctgatca acatcctcaa gcgctgccat atcgcaagcg
    29761 gcttgttgcc cacttaagac ttcggtgcgg ctaccctgtt gctgtagcat cgttttaaga
    29821 agtttcgcac tcgcttcatc gtccattacg gcatagcggg gagagaattc caggcactgt
    29881 tctaccatgc gagtgacatt tttgcctgcc accagcgcaa ctacgcggaa gtgttcggga
    29941 ttatggcgca ccacgtccag cgtgctgcaa ccaatcgagc cggtcgagcc cagaatggtg
    30001 agttgcttca tgaaacatcc agagttgaga cagaataaaa agcaaaacgc cgccagccga
    30061 tccgcaagga tctactgagc ggcgtttttg tcgttcaaga aatcagaact gcatcagttc
    30121 tgcttctttg tctgccagcg ccgcttcaat tttcttgatt gcagcatcag tcagtttctg
    30181 tacatcgtcc tgagaacggc gatcgtcgtc ttcgctgatc tctttatctt tcaacagtgc
    30241 tttcactttg tcgttcgcgt cacgacgcac gttacgtact gcaacacgcg cttgttctgc
    30301 ttcaccacga acgattttgg tcagatcttt acgacgttct tccgtcagcg gcggcagcgg
    30361 aacacggatg tcgctacccg cagagttcgg gttcaggcca agatcggacg ccataatcgc
    30421 tttttcaacg gccggagaca ttgaacgatc aaacacgttg attttcagag tacgggaatc
    30481 ttctaccgtt acgcttgcca gctgacgcag cggcgtcggc gtgccgtaat attccacgac
    30541 aatgccatcc agcaggctgg gagaagcacg acccgtgcgt attttgctga tttgggtttt
    30601 gaacgcttct acgcatttgt ccatgcgtac ttcagcatct tttctgatat cgctaatcac
    30661 gttacgaatc cttgaaaact tgtctcagtc agaccagaca gtcacacaca gtggaagtgt
    30721 gctaagtata gccctgatta aacatattac gggtaagatc ccccgcgata cttacgcgga
    30781 atcttaccct tatttatcca tcacgggaat tattccgtga ttaaagtccc ttctttttca
    30841 cccattacca cacggcgcag cgcacccggt ttgttcatgt tgaaaacacg aatcggtaat
    30901 ttatggtcac gagccagcgt gaaggccgcc aggtccatga ctttcagctc tttttccagc
    30961 acttcgctgt aagtcagttg ctcgtacatg gttgcggttg gatctttcgc cggatcagcg
    31021 gtaaacacgc cgtcaacttt ggttgctttc agcaccacat cggcttcaat ttcgatacca
    31081 cgcaggcaag ctgctgagtc ggtggtaaag aacgggttac ctgtaccggc ggagaggatc
    31141 accacacggt tgttgcgcaa caggctgata gcttctgccc agctgtagct gtcgcacacg
    31201 ccattcaatg gaatagcgga catcagacga gcgttcacat aggcgcggtg cagtgcatca
    31261 cgcattgcca ggccgttcat tacggtcgcc agcatcccca tgtggtcgcc cacaacgcgg
    31321 ttcatacccg ctttcgccag accagcgcca cggaacaggt tacccccacc aatcaccaca
    31381 ccaacctgaa tacccagttc aaccagttct ttgatttcct gagccatacg atccagtatg
    31441 cttgcatcaa taccgaagcc ttcagtgccc tgcagagctt cgccactcaa cttaagcaga
    31501 atgcgtttat agacgggttt tgcattggta gccatgtttc tttcctgaga ctgtcaacga
    31561 ttgaaatgaa ttaatactag cgacattgta cgtcgctttt cagcgcagcc ccacaagggt
    31621 tagctgaatt tacaagatgg gcacaaaaag aagccgccct caggcggctc ctttttgata
    31681 attaagactg cttggacatc gcagcaactt ctgctgcaaa gtcagtctca actttctcga
    31741 tgccttcacc cacttcgaag cggatgaagc cagtcacttc agcgttatgc tctttcagca
    31801 gctgaccaac agttttgctt ggttccataa cgaacggctg accggtcaga gaaacttcgc
    31861 cggtgaattt cttcatgcgg ccttcaacca ttttctctgc gatttctttc ggcttaccag
    31921 actgcatcgc gatatccagc tgtacctggt attctttttc taccacttca gcggatacgt
    31981 cttccggttt gatgaattct ggcttgcttg cagcaacgtg catagcgatg tgtttaacca
    32041 gctcttcgtc agcgccttta gcagcaacca gaacgccgat acgcgcacca tgctgataag
    32101 aacccagaac gtcgccttcc agcgcagcaa cgcggcgaat gttgatgttt tcaccaattt
    32161 tcgctaccag cgcaacacgt tcttcttcga actgtgcttt cagaacttca acgtcagtga
    32221 ttttgccagc aacagctgcg tccagaactt tgtctgcgaa cgcctggaaa ccagcgtctt
    32281 ttgcaacgaa gtcagtctgg cagttaactt ccagaatgat gccgtagttg ccgtcgattt
    32341 tggttttgat cacgccgtca gcagcaacgt tgcctgcttt tttcgctgct ttaatagcac
    32401 cggacttacg catgttttcg attgccagct cgatgtcgcc gttagcttca gtcagtgctt
    32461 ttttgcaatc catcatgcct gcgccagtac gctcacgcag ctcttttacc agggatgcgg
    32521 taatttcagc cattctaaaa tcctcggaga tgtgatctgc ccggcattaa accgcacaga
    32581 tataaaagtg aaaaaggggg ccatatgcag gccccctaac caaacgtgta ctacctggtc
    32641 tataagggct ctcccccttc atatttcgcg ccgcagatgc gtcatcttcg ctcgctcatc
    32701 ccggtcactt actgatgtaa gctcccggga attctcgagc ttgccgcctt tctgcaactc
    32761 gaactatttt gggggagtta tcaagcctta ttactcagct tctacgaagc tttcttccgc
    32821 ctgggaagcc agatcctgag aacggccttc acgtacggtt gcagcaacag cgcccaggta
    32881 cagggtcaca gcacggattg cgtcgtcgtt acccgggata acgaagtcaa caccgtccgg
    32941 atcagagttg gtatcaacga tagcaaatac cggaataccc aggttgtttg cttctttgat
    33001 agcaatgtgt tcgtggtcag catcgattac aaacagagcg tccggcagac cgcccatgtc
    33061 tttgataccg cccaggctgt tttccagttt ctccagctca cgagtgcgca tcagcgcttc
    33121 tttcttggtc agcttgtcga aagtaccgtc ctgagactga gtttccaggt ctttcagacg
    33181 tttgatggac tgacgaacgg ttttccagtt agtcagcata ccgcccagcc agcgatggtt
    33241 cacgaagaac tggtcgcagc tcagagcagc gtctttcacc gcttcgcttg cagcgcgttt
    33301 agtaccaacg aaaaggattt tacctttgcg agaagcaatc ttgttcagtt cagccagagc
    33361 ttcgttgaac atcggtacag ttttctcaag gttgatgatg tgaactttgt tacgcgcacc
    33421 gaagatgaac ggcttcattt tcgggttcca gtaacgggtc tggtgaccga agtgaacacc
    33481 agccttgagc atgtcgcgca tggaaacagt tgccatgatt aaaacctcta tataaaagtt
    33541 ggggttatgc ctccacgtat cccatattac cgaccccaaa gggcaccccg gaatatgtgc
    33601 cgatacgtgt gtgttgttac acaaagtgag attgtcgctt ccgtccagtc tgtgtatacg
    33661 aaatggatcg gaagtccggc gcgctttata ccacaaatac gccgtggaca ccaataattg
    33721 ttggcgctgt gtacagcatc agacgtcgaa ttttctatta tagaaaacct tcagtggcac
    33781 gtttggcgaa attcagaatg attctcaatt tgcccgggtg tgataccatt gacggcactt
    33841 acatatatat tgtcggtatc accgacgctg atggacagaa ttaatggcta tctcaatcaa
    33901 gaccccagaa gatatcgaaa aaatgcgcgt cgctggccga ctggctgccg aagtgctgga
    33961 gatgatcgaa ccatatgtta aaccgggcgt cagcaccggc gagctggatc gcatctgtaa
    34021 tgattacatt gttaatgaac aacacgcggt ttcagcctgc ctcggctatc acggttatcc
    34081 gaaatccgtt tgcatttcta ttaatgaagt ggtgtgccac ggtattccgg acgatgctaa
    34141 gctgctgaaa gatggcgata tcgttaacat tgatgtcacc gtaatcaaag atggtttcca
    34201 cggcgatacc tcgaaaatgt ttatcgtcgg taagccgacc atcatgggcg aacgtctgtg
    34261 ccgtatcacg caagaaagcc tgtacctggc gctacgcatg gtaaaaccag gcattaatct
    34321 gcgcgaaatc ggtgcggcga ttcagaaatt tgtcgaagca gaaggcttct ccgtcgttcg
    34381 tgaatattgc ggacacggta ttggtcgcgg cttccatgaa gaaccgcagg tgctgcacta
    34441 tgactcccgt gaaaccaacg tcgtactgaa acctgggatg acgttcacca tcgagccaat
    34501 ggtcaacgcg ggtaaaaaag agatccgcac catgaaagat ggctggacgg taaaaaccaa
    34561 agatcgcagc ttgtctgcac aatatgagca tactattgtg gtgactgata acggctgcga
    34621 aattctgacg ctacgcaagg atgacaccat cccggcgata atctcgcacg acgaataaaa
    34681 tgaagccggc gaatgccggc ttttttaatg cgataattta atcttatggg tggcgcacaa
    34741 tgaataccct tccagaacag tacgcaaaca ccgctctccc caccctgccc ggtcaaccgc
    34801 aaaatccatg cgtctggccc cgtgatgaat taaccgtcgg tgggataaaa gcccatatcg
    34861 atactttcca gcgttggctg ggtgatgcct ttgacaatgg gatctctgca gaacagttga
    34921 ttgaggcgcg caccgagttt atcgaccagc tcctgcaacg attatggatt gaagcgggat
    34981 tcagccagat tgccgacctg gcattggtcg ccgtcggtgg ctacggtcgt ggcgagctgc
    35041 atccactttc tgacatcgat ttactgattt taagccgtaa aaagctcccg gacgatcagg
    35101 cgcaaaaagt gggcgagctg ttaacgctgc tctgggatgt aaagctggaa gtcggtcata
    35161 gtgtgcgcac gcttgaagag tgcatgctgg aagggttatc ggatttaacc gtcgccacca
    35221 atttgatcga atcccgctta ttaattggcg atgttgcgct atttcttgaa ctgcaaaaac
    35281 atattttcag cgaaggattc tggccttccg acaagttcta cgcggcgaaa gttgaagaac
    35341 agaaccagcg ccatcagcgt taccacggca ccagctacaa tcttgaacca gacatcaaaa
    35401 gtagccctgg cggcttgcgc gatatccaca ctctgcaatg ggtggcccgc cgtcattttg
    35461 gcgcaacatc gctggatgaa atggttgggt ttggcttctt aacctcagcg gagcgggcgg
    35521 aattaaacga atgtctgcat atattgtggc gtattcgctt tgccctgcat ctggtcgtca
    35581 gccgttacga taatcgcctg ttattcgatc gccagcttag cgtcgcccag cgtctgaatt
    35641 acagtggcga aggtaacgaa ccggtcgagc ggatgatgaa ggattacttc cgcgtgacac
    35701 gccgcgtcag tgaactcaac cagatgctgc tgcaactgtt cgatgaagcc atcctcgccc
    35761 ttcccgccga cgaaaaacca cgtccaatcg acgatgagtt tcagctacgc ggtacgctaa
    35821 tcgacctgcg tgatgaaaca ctatttatgc gccagccgga agccatcttg cgtatgttct
    35881 acaccatggt gcgcaacagt gcgatcaccg gcatttactc caccacactg cgccagttac
    35941 gccatgcccg tcgccatctg caacaaccgc tgtgtaatat tccgcaagca cgaaaactgt
    36001 ttttgagcat tctacgtcac cccggagcgg tgcggcgcgg gctattgcca atgcatcgcc
    36061 atagcgtact cggcgcgtat atgccgcaat ggtcacatat cgtcgggcag atgcagtttg
    36121 atctgttcca cgcctacacg gtggatgaac atactatccg cgtgatgctg aaactggaga
    36181 gttttgccag tgaagaaacg cgccagcgcc atccgttgtg tgtgaacgtc tggccgcgcc
    36241 taccgtcaac tgagctgatt ttcatcgccg cgctgtttca cgatatcgcc aaaggacgcg
    36301 gcggcgacca ctctattctc ggtgctcagg atgtagtgca ttttgccgaa ctccacgggc
    36361 tgaactcacg cgaaacacag ctggtcgcct ggctggttcg ccagcacttg ctgatgtcgg
    36421 tgacagctca gcgtcgcgat attcaggacc cggaagtcat caagcagttt gccgaagaag
    36481 ttcaaacgga aaatcgtctg cgctatctgg tatgcctgac agtggctgac atttgcgcca
    36541 ccaacgaaac gctgtggaat agctggaagc aaagtctgtt gcgcgaactc tatttcgcca
    36601 ccgaaaagca gctacgacgc ggaatgcaaa acacgccgga tatgcgcgaa cgggttcgcc
    36661 atcaccaact ccaggcgctg gcactactgc gcatggataa catcgacgaa gaggtgctgc
    36721 accaaatttg gtcacgctgt cgcgcgaact attttgtccg tcatagccca aatcaactgg
    36781 cctggcatgc gcgccattta ttacagcatg atttaagcaa accgctggta ttgcttagcc
    36841 cgcaggctac gcgtggaggc accgagattt ttatctggag cccggaccgc ccttacttgt
    36901 ttgctgctgt ttgtgccgag ttagatagac gcaacttaag cgttcacgac gcacaaattt
    36961 tcaccactcg cgacggtatg gcgatggata cctttatcgt gctggaaccc gatggtagcc
    37021 cgctatccgc agatcgtcat gaggttattc ggtttggtct ggagcaagta ctgacacaaa
    37081 gtagctggca gccaccacag ccgcgtcgtc aaccggctaa actacgtcac tttactgttg
    37141 aaaccgaagt gacgtttttg ccaacccata ccgaccgcaa atcgttcctt gagcttatcg
    37201 cgctcgacca gccaggactg ctggcgagag tcgggaaaat ttttgccgat ctgggaattt
    37261 cgcttcatgg tgcccgaatt acaaccattg gcgagcgagt agaagattta ttcataattg
    37321 ccaccgctga ccggcgtgca cttaataacg agttgcagca ggaagtgcat cagcggttga
    37381 cagaggccct caatccaaac gataaagggt gatgtgttta ctgatatgaa aagagtttaa
    37441 caatgcagca gttacagaac attattgaaa ccgcttttga acgccgtgcc gagatcacgc
    37501 cagccaatgc agacaccgtt acccgcgaag cggtaaatca ggtgatcgcc ctgctggatt
    37561 ccggcgcact gcgtgtagcg gaaaaaattg acggtcagtg ggtgacgcat cagtggttga
    37621 aaaaagcggt gctgctctct ttccgtatta atgataatca ggtgatcgaa ggggcagaaa
    37681 gccgctactt cgacaaagtg ccgatgaaat tcgccgacta cgacgaagca cgtttccaga
    37741 aagaaggctt ccgcgttgta ccacctgcgg cggtacgtca gggtgcgttt attgcccgta
    37801 acaccgtgct gatgccgtct tacgtcaaca tcggcgcata tgttgatgaa ggcaccatgg
    37861 tcgatacctg ggcgaccgtc ggttcttgtg cgcagattgg taaaaacgtc cacctttccg
    37921 gtggcgtggg catcggcggt gtgctggaac cgctgcaggc taacccaacc atcattgaag
    37981 ataattgctt catcggcgcg cgctctgaag tagttgaagg ggtgattgtc gaagaaggtt
    38041 ccgtcatttc catgggcgta tacattggtc agagcacccg tatttacgac cgtgaaaccg
    38101 gcgacatcca ctacggtcgc gttccggcgg ggtctgtggt tgtttcaggt aatctgccgt
    38161 cgaaagatgg caaatacagc ctctactgtg cggttatcgt taagaaagtt gacgcgaaaa
    38221 ctcgcggcaa agtcggcatt aacgaactgc tgcgtaccat cgactaaaag tatgcacacg
    38281 ggcagcacga cgctgcccga ttgctttctg gttcagaata attcgtccta ctcattcttc
    38341 ctcccccctt ttttaaatac tttttcatct tgtttcggac tgtcatgtaa ttcgcaaaac
    38401 gaagataaat gtgctaatcc atcccatgga atatcgccac aataaagcag gtaatttttt
    38461 gatttttacg ccatgataga agtctggaag aaaaaggctt tctgcgatca cagtcgagcc
    38521 gggcgggcca ggccaaatgc taaaataagc acattttact gactcatttt gaggtaacgc
    38581 tatgtacgac aatctgaaaa gtctgggtat taccaatcct gaagaaattg atcgttacag
    38641 cctccggcag gaagccaaca acgatattct gaaaatctat ttccagaaag acaaaggcga
    38701 gtttttcgcc aagagcgtta agtttaaata tccgcgtcag cgtaaaacgg tcgtcgctga
    38761 tggtgtgggt cagggttata aagaagtcca ggaaatcagc ccgaatctac ggtatatcat
    38821 tgatgagctt gatcaaatct gccagcgtga ccgcagcgaa gttgatctta agcgtaagat
    38881 cctcgacgac ttacgtcacc tggagtcagt cgtaaccaat aagatcagcg agattgaagc
    38941 cgatctggaa aaactaacgc gtaaataatc ccgaattgac ggttaactcg ggcattcccc
    39001 tctccccttc gggctgaggg gaatgagcca gcgccgcctc aaccttaacg cataacctac
    39061 cgctcttcat ccagttgtaa cgccacatac agcagcaacc tgtcatcaaa attgcccaaa
    39121 tcaagcccgg tcagttccga tatacgatta agccgatact ccagggtatt acgatgaata
    39181 aacaacgcct ttgacgttgc cagcggttgc acattgtgac gaaaccacgc cgccagcgtt
    39241 cgtcgcagca agccgttatt gtccatcgct ttcagccgcg ccagcggtcg cgccagttcg
    39301 ttggcctgcc agtcgccacg caaactgtcg agtaacacag gtaacatcag atcctgataa
    39361 aaatagcagc gactttctgg catccgctgt ttacccacca ccatcgtcgt tttcgccgta
    39421 cgataggatc gggcaatact gccaggaccg gtaaaatagt tgcccagtga aacgcgaaaa
    39481 cgcagctggc cgtactcttt catgcgggta atcagttgtt caactcgctt acgatgatct
    39541 tctacatccc agcgcccaaa agagttcaac gccggtttca acaccaccat ttcggttagc
    39601 gagacaatcg ccaccagatt attacgctcg ggcgtagtca gcgcgttttg cagttgttgt
    39661 aactccgcca ttgcgctgtc cacaccaagc tgaccgctgt cgacctcaac aatagccacc
    39721 actcgcggtt gattgagatc gatccccagc cgttgcgccc attcagtaag tgcgggagta
    39781 ttctcctctg cctgaatcag gttcatcacc agttcctccc gcaaacggct atcctgcgcc
    39841 aacaagtgca tcaaccgcga ctgttccagc atcatttcag ccgtcatgca gaccagttcg
    39901 ccatatttac gcagattctc tggttcacct gtcaggccaa ttacgccgac aatttcacct
    39961 tccagccgta acggtagatt aatcccttgc cgcacaccgt gcagatgacg tgccaccgcg
    40021 tcatcgatat cgacgactcg tccctgtgaa agtaccagca atgcaccttc gtgcaattca
    40081 ccaatacgct cacgatcgcc gctgccgata attcgcccac gggcatccat tacgttgata
    40141 ttggtatcga tgatgcgcat ggtacgtgcc acgatatcct gcgccatttt ggtatcaaga
    40201 tgccagccag ccataagtcc tccgttatgc acggcttagc ataaggaagt acgtaacgta
    40261 cggcattgtg caaatgccta aaggatgatg aagatgtatg gagttgtggt gaagttcaca
    40321 gattgtaagg agaccccctt cccgttttca ggaaggggtt gagggagatt actgcattaa
    40381 caggtagatg gtgctgtcgc cgcgctgaat gttcagtgcc agcacagacg gtttgctgtc
    40441 gagaacttta cgcagttcag cgatgttttt cacagcctgc tggttcgcgc caataatcac
    40501 atcacctttc ttcaggccga tctgcgcagc cggagtgccc gttttcacgt tgttcactac
    40561 cacgccctga tctttgcctt tgttgctcat ctcagcgcct tcaatgccgt tgaagatgga
    40621 gctggaatca acctgattct ggctgctctg ctgcagttcc agattcacgt tgacctgctt
    40681 accgtcgcgc agcaagccca gggtcagttt gctgcccacc ggcatagtac ccacttgagc
    40741 acgcagtgcg gcaaagctgc tgatcggctt accgttcagt gaggtgatca catcacccgc
    40801 tttaatgccc gcttttgcag cggaggaatt aggcagaacc tggcttacga aagcaccgcg
    40861 ctgggcgtca actttcatcg ctttcgccag ttcggagttc agctcagtcc ccataatacc
    40921 cagctcaccg cgtttcacct ggccgtattc caccatctgc gaggtcaggt ttttcaccat
    40981 gttactcggg atagcaaaac cgataccgat gttaccgccg tccggtgcta ggatcgcggt
    41041 gttgataccg atcagttcgc cgttcaggtt aaccagcgca ccaccggagt taccacggtt
    41101 gatcgctgca tcggtctgga tgaagttttc gtagttttcg gcattcaggc cgctacgccc
    41161 cagcgcagag acaatcccgg aagttaccgt ctcgcccaga ccaaacgggt taccaatcgc
    41221 tacggtgtaa tcacccacgc gcagtgcatc agaatccgcc atcttaattg cggtcaggtt
    41281 tttcggattc tggatttgga tcagcgcgat atcagagcgc ggatctttgc caaccatctt
    41341 cgcgtcgaac ttacggccat cgctcagttg aactttaatg accgtcgcgt tatcaacaac
    41401 gtggttgttg gtgacgacat agcctttatc ggcatcaatg atgacgccgg aacccagcgc
    41461 catgaatttc tgttgctggc cgccaccatt accgccctgg ccaccctggc agaacggaga
    41521 gctctggaac ggagaacctt cctggcagaa cggagaatca tcaccgaaga actgctggaa
    41581 attacgcggc atacgcggcg tattaacggt tgtgctacct tctacgttaa tgctgaccac
    41641 tgaaggcatc accttttcga gcatcggtgc aaggcttggc atctgctggg ctgtcgttgc
    41701 tgaagaagtc tcagccgccg ttgcagagag cggagataac gccaaaccta aactcagagc
    41761 cagtgcactc agtgctaatg tggttttttt catgtatttc agtctcgatt aacagataac
    41821 gcaaaattgc tgtgttcttc agattcgttt tatagcgcga agttccgaac taaagtttct
    41881 gcaaaaggta aaaatttatt gttcgtcttt acaaaagcct ggttattgtt ctacggccat
    41941 cagacgtcgg tattcatccc aggcatagag gtcggtcata ccgctgatat aatcctgcag
    42001 caggcggcaa cggtaataat attcccatag cggaaactca ggagaatctg acggtaattt
    42061 actgacagcc tcgacatagg ccagccgatg gcgcgtcgag agtttgtgga ataagcgaga
    42121 ttcaataggg gaacgtttca cccgttcttt ttctaccaat tcagtaaagt ctgataatga
    42181 caggcttaat aaagggcgat aaatctctaa taatccgcta atgacccgat agccctgcaa
    42241 ttcaagctgc tcgacatctg gatggctaaa cacatgtttt acagcgacat ttttatatag
    42301 cttaagaaga tcgctgcatt cgctggcatc ttccaataat gcatgattaa acgttccggc
    42361 gaaaatcgca ggcagattat caataaatcg ttgtgccgcg tagggtacca gtttatttag
    42421 ggtgtttacc cgtaaataca taaaaaactg atcttccgta ctgcggctta aactatttga
    42481 gcgtgatttt tcccaggcat tttcaaccac cagcgaaaag agcgaacctt tctcatgctg
    42541 gccccacgcc tcgtgcaaat gatgataaag ctgctcaacg gtaaatattc ttttctctac
    42601 cgcatcttca agatctgcca cacaatagga gatgtcgtcg gcagcttcca taatccacgt
    42661 taatggaaaa cgactgtaaa gcgccaaatt aagttcttta cgcaaccggg caatataggc
    42721 ttcttcagaa agataataac ccggcttttt cattaaatag tgatgtgtct caggcgtttc
    42781 gccacgccac cacgctggac gggtatattt taaaataccg ccaacctgcg cccaggtgag
    42841 attcatccgc atcaatgtat gcaccagacg aatgccttgt gcattcccct caaaatgaca
    42901 taagtcctga cgaatcttgc gccgcagctc gttaagcggt tcttccccgt cccgtaaacg
    42961 tagtaccgcc acgctgcagc gatcgtcagt cagaggctgg ctttcggcat cttccgggtg
    43021 caaacgctgg cgaaaccagt catttatcgc cgcttcgcca aaatgaccaa acggcggatt
    43081 gccgatatcg tgcatcaggc atgacatctc aacaatgctt tcaaaaggtc cggtcagttc
    43141 atccaggccg tatgcttcca gcaatttaag ctctttcaga cggcttaaaa tttctttggc
    43201 gatgtagcgc cccacctgct ggacttccat cgagtgggta agacgcgtgc gcacggcggc
    43261 attgcgctcc agtggaaaaa cctgggtctt ttgttgcaga cgacgaattg ccggagagtt
    43321 gatgatacgc ccgcgatcgc tctcgaagat ccgcaggatc tcatgttcgg ttttaacgcc
    43381 ctgcggtgaa cggtaacgac gatgccagtt tatttttttt cggaaatcaa tctgtgccat
    43441 gtcctctccc gcgtgagaaa tacgcttccc cgtaagcgca tggtaaacta tgccttcaaa
    43501 tcgggcttat cgcgagtaaa tctatgaaaa tcggcatcat tggtgcaatg gaagaagaag
    43561 ttacgctgct gcgtgacaaa atcgaaaacc gtcaaactat cagtctgggc ggttgcgaaa
    43621 tctataccgg ccaactgaat ggaaccgagg ttgcgcttct gaaatcgggc atcggtaaag
    43681 tcgctgcggc gctgggtgcc actctgctgt tggaacactg caagccagat gtgattatta
    43741 acaccggttc tgccggtggc ctggcaccaa cgctgaaagt gggcgatatc gttgtctcgg
    43801 acgaagcgcg ttatcacgac gcggatgtca cggcatttgg ttatgaatac ggtcagttac
    43861 caggctgtcc ggcaggcttt aaagctgacg ataaactgat cgctgccgct gaggcctgca
    43921 ttgccgaact gaatcttaac gctgtacgtg gcctgattgt tagcggcgac gctttcatca
    43981 acggttctgt tggtctggcg aaaatccgcc acaacttccc acaggccatt gctgtagaga
    44041 tggaagcgac ggcaatcgcc catgtctgcc acaatttcaa cgtcccgttt gttgtcgtac
    44101 gcgccatctc cgacgtggcc gatcaacagt ctcatcttag cttcgatgag ttcctggctg
    44161 ttgccgctaa acagtccagc ctgatggttg agtcactagt gcagaaactg gcacatggct
    44221 aagtcactgt tcagggcgct ggtcgccctg tcttttcttg cgccactgtg gctcaacgcc
    44281 gcgccgcgcg tcatcacgct ttctcccgcc aacactgaac ttgcctttgc cgccgggatc
    44341 acgccggttg gggtcagcag ctattccgac tatcctccac aagcgcaaaa gattgagcag
    44401 gtttccacct ggcaggggat gaatctggaa cgcattgtcg cgctgaaacc cgatctggtg
    44461 attgcctggc gtggaggtaa tgccgagcgg caggttgacc agctggcttc gctgggaata
    44521 aaagtgatgt gggtcgatgc gacaaacatt gaacaaattg ccaatgcgtt acgtcaactg
    44581 gctccctgga gtccgcaacc ggataaggcc gaacaagccg cgcaatccct gctggatcaa
    44641 tacgcgcaat tgaaagcgca atatgctgat aaacctaaaa aacgtgtttt tctgcaattc
    44701 ggcattaatc cgccatttac cagtggaaaa gagtcgattc aaaaccaggt actcgaagtt
    44761 tgtggcggag aaaacatctt taaagacagc cgggttccct ggccgcaagt tagccgcgaa
    44821 caggtgttag cacgctcgcc acaggcgatt gtcattacag gcggaccgga ccaaattcct
    44881 aaaatcaaac aatactgggg tgaacagctc aaaattcccg ttattcctct cacgagtgac
    44941 tggtttgaac gtgcaagccc acgtattatc ctcgctgcac aacagctctg taatgcgctt
    45001 tcacaggtag attagcgcct gaccttttta ggtgctcagt ggggattgaa caatgctcgt
    45061 ctattggctg gatatagtcg gcacagcggt atttgccatc tccggcgttt tgttagccgg
    45121 aaaattgcgt atggaccctt ttggtgttct ggtactgggc gtggttaccg ctgtaggcgg
    45181 cgggacaatt cgcgacatgg cgctggatca cggcccggta ttttgggtga aagatcccac
    45241 cgatctggtc gttgcaatgg taaccagcat gctgaccatc gtgctggtgc gccagccaag
    45301 acacttacca aaatggatgt tgccggtgct ggacgccgtt ggtctggcgg tgtttgtcgg
    45361 cattggcgtg aataaagcct ttaatgcgga agccggtccg ttaatcgcgg tttgtatggg
    45421 cgtcattact ggcgttggcg gcgggatcat tcgtgatgtt ctggcccgcg aaatccccat
    45481 gattttacgt acagaaatct acgcaactgc ctgtattatc ggcggtattg tccacgctac
    45541 ggcttattac acattttccg taccactgga aacagccagt atgatgggca tggtcgtgac
    45601 gctattgatt cggctggcgg ctattcgttg gcatcttaag ctaccgacgt ttgcgctgga
    45661 tgagaatggg cgttgaagag agaagatgtg ccggatagtt tatccggcac atgaacaaca
    45721 gattagatac taaaggaaga accgcaaccg caggtgcttt tcgcgttcgg gttggtcacg
    45781 atgaaacgag aaccttccag tccttcggta tagtcaacgg aaccgccgac cagatattgc
    45841 aggctcatcg gatcaaccac caggccaacg ccctgttttt cgatggtcat atcgccttcg
    45901 ttcacctgat catcaaaggt gaaaccatac tggaagccgc tgcaaccgcc accggtgata
    45961 tacacgcgta atttcagatt cgggttatct tcgtcagcga tcaggctttt aactttgttg
    46021 gctgctgcgt cggtaaactc cagcggcagt gctacgtcat cactcatatt ttgctccaaa
    46081 cgacatcggc aattgggcaa attctaaccc aataataatc gccattatct aataccctgg
    46141 tatttcgttc aagtattctc gctggctgat gcggccttgc ttcgcgccag ttgctcagct
    46201 tcctgttttg ccagcgtgcg cgcaagaatc gccgagtata gcggtttccc gccggtaaat
    46261 tgcgctaata gtgttgcgcc aagaccggta ataatcattg gcaaaatgag ctggtagtta
    46321 tcggtcatct ccagaaccag aatgatcccc gttaacggcg cgcgaataga tgccgccagt
    46381 aacgccccca ttccggcaat agcaaacgtc cccgcctcaa ggtgatattg cggaaacagc
    46441 tcaacggcaa ccattccgaa agcggttccc agcacagtac ccagcgccag catcggggca
    46501 aaaataccgc ccggcgcgcc ggaagagaag cagagtaagg tggtaatgac ccgcgcgacg
    46561 aagataaaca ccagcattcc catgctgaaa ttccccgcgg tagcgatagg aatcaggtta
    46621 aaaccgccgc ccgacgttgc tggtgccaca aaccccagca atccacacag accgccaatc
    46681 gcaccaccca tcagtaccca tttggtaata ttgccgccgt gcacacggtg cagcaaatcc
    46741 tgcatcccca gcacccattt attaaaaata gggccgaaaa tgccaaaaat aataccgagg
    46801 atcagataaa gccacagcgt attaagcggc gcgtcagaaa gtttaccgac gtcaatcaac
    46861 gcaacttcat gattaaaaat ccggtacata atggtcgaca taatgacgcc aataaatacc
    46921 gctttaatcg aaattaacgt atagcgaaac tgcggacgca tctcttcgat aataaacaaa
    46981 atacccgcca gcggcgcgtt aaaggccgcc gccagacccg ccgccgcacc ggttgccaac
    47041 agcgtatggc gagcttcatc acctttcagg cggaaaacat cgagtaccat ccgcccgata
    47101 ttaccgccga tctgcacggt tggcccttcg cgccccaaca ccatgcctcc gccgagtgtc
    47161 cccagcccgc caaagaattt caccggcaat acacgccacc agcgaacagg acgttgatct
    47221 tccagcgccc cttcaatttc cgggatcccc gacccccctg cttcgggcgc gtatttgcgc
    47281 accaaaaagt agccgaacat cgccagcacc gccgaacaga gaaaagcaac ggttaacagt
    47341 agcggataat tatcagcagt atgtaccagc gcccccatac gttggttctg caaccaggcg
    47401 acacctttgt caaaagcaac cgctgccagc ccgacaagcg tgccgacgac tgccgccata
    47461 aacaaaatgg ctaacggggt tttatcgcgc tcaagaagtt ggcgaatcag ttgtctgcgt
    47521 cgcaggcgcg cggcctgcgg tgtttctaaa gagggagtat cagttttcat ccaatgatca
    47581 cttattggtc atacaaataa gatgacggca ttttactcgc acgttccatg aaaatccttt
    47641 gcaaagcgta atgtttcaaa tgcacaaaac ttttatactc taagaaatca ggattgcacc
    47701 agtacaagca gcctgatgtt tgacgagtat ttaacttgtt atgaataaca tagaatagca
    47761 gccattcact ttttctacga atcaggaacc ctccatgagt aagtctgaaa atctttacag
    47821 cgcagcgcgc gagctgatcc ctggcggtgt gaactcccct gttcgcgcct ttactggcgt
    47881 gggcggcact ccactgttta tcgaaaaagc ggacggcgct tatctgtacg atgttgatgg
    47941 caaagcctat atcgattatg tcggttcctg ggggccgatg gtgctgggcc ataaccatcc
    48001 ggcgatccgc aatgccgtga ttgaagccgc cgagcgtggt ttaagctttg gtgcaccaac
    48061 cgaaatggaa gtgaaaatgg cgcaactggt gaccgaactg gtcccgacca tggatatggt
    48121 gcgcatggtg aactccggca ctgaagcgac aatgagcgcc atccgcctgg cccgtggttt
    48181 taccggtcgc gacaaaatta ttaaatttga aggttgttac cacggtcacg ctgactgcct
    48241 gctggtgaaa gccggctctg gcgcactcac gttaggccag ccaaactcgc cgggcgttcc
    48301 ggcagatttc gccaaacata ccttaacctg tacttataac gatctggctt ctgtacgcgc
    48361 cgcgtttgag caatacccgc aagagattgc ctgtattatc gtcgagccgg tggcaggcaa
    48421 tatgaactgt gttccaccgc tgccagactt cctgccaggt ctgcgcgcgc tgtgcgacga
    48481 atttggcgcg ttgctgatca tcgatgaagt aatgaccggt ttccgcgtag cgctagctgg
    48541 cgcacaggat tattacggtg tggaaccgga tctcacttgc ctgggcaaaa tcatcggcgg
    48601 tggaatgccg gtaggcgcat tcggtggtcg tcgtgatgta atggatgcgc tggccccgac
    48661 gggtccggtc tatcaggcgg gtacgctttc cggtaacccg attgcgatgg cagccggttt
    48721 cgcctgtctg aatgaagtcg cgcagccggg cgttcacgaa acgctggatg agctgacaac
    48781 acgtctggca gaaggtctgc tggaagcggc agaagaagcc ggaattccgc tggtggtaaa
    48841 ccacgttggc ggcatgttcg gtattttctt taccgacgcc gagtccgtga cgtgctatca
    48901 ggatgtgatg gcctgtgacg tcgagcgttt taagcgtttc ttccatatga tgctggatga
    48961 aggtgtttac ctggcaccgt cagcgtttga agcgggcttt atgtctgtgg cgcacagcat
    49021 ggaagatatc aataacacca tcgatgctgc acgtcgggtg tttgcgaagt tgtgatgatt
    49081 ttgcctcgcc caccgggcga ggcattttat taacggctct gctttctcaa caaatagata
    49141 aaatatggcg cgccgataaa ggtcgacagc agccccgccg ggatctggaa cggaaacagc
    49201 accatccgcc cacaccagtc agcgaacacc agcagtaaac cacccaccag cgccgaaatc
    49261 acgatgtgcg gcatcgtccg tcgaaagccc atcatccgcg cgatatgcgg tgccattaaa
    49321 ccaacaaaac tcaacggtcc aatggtcatc gtcgcggtcg ccgtcaggca agccgctaac
    49381 agcagcagcg caattcgcgt cggcgttaac gccaccccca cggcccgcgc ggtatcacca
    49441 cccagcggta aaatggtcag ccagcggcgg cacagcgggg taatcgccag caaaatcacc
    49501 atcacaattc cggtgcgcca gacctgcgca tcggtggcgt tgtaggtcga accagagatc
    49561 caggtcagca cttgcgccat tcgcgggtca ccactcgcct gcaacatcat taaaagcatg
    49621 gtgaatgcgg tacttaacgc catccccgcc agtaacatgc ggtgtgggga aaatccaccg
    49681 cgcccggcgg cgatcataat aatcaacagc gtcaccgccg caccgatact tcccgcgggt
    49741 aacagccagc caaaagcatt acccggtacc agaaacagca tcagtaccac gccaaacgcc
    49801 gcgccagagc taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga
    49861 ataatacagc ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc
    49921 cagcgccagg gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca
    49981 tcacgaccaa acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg
    50041 gcaaacgcca gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc
    50101 gcgctaatgc tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca
    50161 gtgaccgaac cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg
    50221 gaaagccaga ggatcagcgc accaatcaac gacgccagca tcagtcgcgg cagcagacgc
    50281 cgcgccccca gcatttttgc cagcaacggc gcgaacaacc cgataaagcc gataatcccc
    50341 accgcgttca ccagcagcgc actgatgaca atagccagcg acagcgccgc cagacgcgcc
    50401 agcgacaagg ctagcccgag attgcgcgcc acgccatcat caagccccat cagtgttaac
    50461 ggacgaagta gcagcaacgt cagcatcaca ccgcccagca actgcggcca taaacgctca
    50521 acaccgcccc agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagt
    50581 tggtcatggt ggaagataac cagtaactga ttgattgccc cacaataaag gctcactacc
    50641 agccccgcga ggatcagcgt taccggcgaa agccgtttcc cccacgcgac gccaaagacg
    50701 attaagccaa caacgcaagc gcccgccagc gcagcaaact gactcgccat cgcccctggg
    50761 atcgcccaga gcgtagtgac ggtaatcccc agttgcgcgc ctgtagcaac gccaagtgtc
    50821 gtcggttccg ccagcggatt acgcagcact tgctgaaaca gcacgcccac cagccccagg
    50881 cctgcgccca ccagcagcga aatcgccaga cgcggcagca agctgtagtg aaaaatcatc
    50941 tgctcgatga cgtcaatatc cggcgaccag gcagcctgcg cccactggct acgcggcagc
    51001 gcctgcgaga agttcatcca ggtcagcgcc gtagcggcaa tcaccagcaa cgccagcaat
    51061 aatgccggga aaagcgcaat acgtttactc acgctttacc tccaatggcg ttatccagaa
    51121 cgcgcacaaa gtgcattgcc gagagagttg caccataaaa ccagactgcg ggtacgcgct
    51181 gaaagcgtcc ggcgcggaca aacggcatgg cctgccacag cggcgttgcc attagcgcat
    51241 ccatgtcttt gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca
    51301 gacgatcgat gctgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg
    51361 ggatgccgta ctcatcaaga atttcctgga acaagctgtt tggaccgaag accagcatat
    51421 ggcgcggatc gataagcgtc gtcagcaata acggacgcgc accacgcttc acaaagcggg
    51481 ggttcatgct gcggataaag tcttcatatt gcgctaaatg cgtttctgct gcgctttgca
    51541 ggttaagtaa atccgccatt tccgtcagcg atttacgcgc catcgccagc ggctgtttgc
    51601 cgtcgctgaa gttaaatcca cgccccggcg caatacgagc cagcatttct ggcgaaggac
    51661 catatcccgc cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcgaggttag
    51721 gttctgtacg caaaccgacg tcaatcaccg attcaggtag tggtggttcg ctgacccaca
    51781 gtcgatagtt gatggtgtct gccacgccat aaggcacgat gccgagcgcc agtagtaatt
    51841 ccaccggcag ccactccagc gccacaatac gatttgggtc aatagccgcc gcgtgggcgg
    51901 tattcatctg ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa
    51961 taagaggtaa gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg
    52021 cagaataccc atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc
    52081 aggcgttccc tgagcaatca tttcaccgcc gcgcagggcg accagataat cacagtaacg
    52141 cgcagccata ttgatatcgt gcagcacggc aatgaccgtc aggccacgct cctgacttaa
    52201 acggtgcacc agcgccagca catcaacctg gtgggcgata tccaacgccg aagtcggttc
    52261 gtcgagcaac agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg
    52321 ttcgccgcca gagaggctgt ctaccagccg atgcgccagc ggttttaagc caaccagcga
    52381 gatagcttcc tcaacttttt cgcgatctgc cgcaccaaag cgccctagtg cgccatgcca
    52441 cgggtaacga ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg
    52501 ctgcggcaaa taagccactt tgcgggcgaa cgctttgctg ctccagcttt ccagcggctg
    52561 ggcatcaaga agaatctccc cttccgacgg cggctgatga cggccaagca ttttgagcag
    52621 agtggattta ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt
    52681 taacgacagc ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc
    52741 aaaagtggta tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaacaggca
    52801 cggaaatccg tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga
    52861 cgttctgcgc cccagaagca gccataagtg ttaaagcagc tggcgacgta ttcacgatcg
    52921 aacaggttgt taacatgcag cgccacgttg gagcccgcca tgccgactcg cgccagatca
    52981 taacgcacta acgcatccac gaccgtataa cttcccactt taaaggagtt agccggatca
    53041 ccatagctgg agccagtata acgaccaccg gtgcccagcg tcagacctga aagcggaccg
    53101 tcaaagaagg tatagtcagc ccacagcgaa gccatgtgtt ttggcacctg tgcaggcgta
    53161 ttgcctttat aggtagtatc ggtggtgtat tccgcatcgg tgtaagtata agaaccgact
    53221 acgttaacac tcgccgacag cgccgctttc gcttcgattt ctacgccacg tgcgcggatc
    53281 tcgccacctt caaccgagaa gaaggaaccc tcagggtccg ccatcaggtt gttggtttta
    53341 gtgagattat acacggcacc agtaactaca atcggacgat cttccggtac atatttcacg
    53401 ccgacttcat actgcttacc tttagacggt gcgaaaatat taccatcctt cccaacttgc
    53461 gaagaaggtt caaacgattc gctatagcta aagtaaggtg ttacgccatt atcaaacagg
    53521 tagttaacac caccacgcca ggtaaactgt ttgtcatcac gtttatcggt cgtcccggca
    53581 acgcggttaa gagattcttg atctgcccag tcataacgac cgcccagggt gaccagcact
    53641 ttatcccact gcgcctgatc ctgaacataa acgcccgttt gtttctgctt attcagaatg
    53701 cggtaagggc cggagtttgc cggatctttg gcattgaagt cgaaatcggt attcaccgga
    53761 ttgtacagat cgagcagcgg tacggagtcg tcgtaaccaa accaggcgtt gatgtcatta
    53821 cgcatacgca taaagtcgac accggtcagc agggtgtggt cgatatcgcc agtggcaaac
    53881 ttgctctgca actgggtatc aacggagaag ttttgcagct tctcatcatc aacgacgtat
    53941 ttacgtgcca gataatggcc tttatccgct ggcgctaatg ccgcacactg tttgctgtaa
    54001 gcattcgccg gatcggagca gacgccgtaa ccataaacgc tgttttgcga ggttttgttt
    54061 tcagcaaagc gcaggttctg acgcacagta aaggtgtcgt taaattcgtg atcgaagctg
    54121 tagcccacca tcttctcatt acgagaatag gtgttgttct tcgccccttc gttaaagtct
    54181 gtcggcagac gcttaccgtt cggcagcggc tcaacggttc cctctttcgg caaccagccg
    54241 taataaccgg tttccggctc gttctggaag taagaaagga aggtgaaatt ggttttatca
    54301 tccggacgcc aggtgaacgc cggtgcaata gcataacgct gctcttctga ccctttctgc
    54361 tgggcattgg cagaacgcgc aagaccggtc aggcgataag agtaaacgcc gtcatcatcc
    54421 agcgcatcgc taaagtcaaa accagtctgg aacaggctgt cagtaccggc tttaaactga
    54481 acttctttca gcggttcggt ggtcggacgc ttgctgacca tattcaacag gccgccagga
    54541 ctgcttttac cgtaaagcac ggaaaccggg ccacgcataa tttcagcgcg ttccagcata
    54601 tacggatcaa tgaccgcatc gttatagaag ttgccctgca acttcaggcc attcagatag
    54661 ttattctggc tttggccttc tgccgcaaaa ccgcgaatga tcaggtggtc ataggtgttg
    54721 gatgcgccac gcgtaccaac agagacaccc ggcgtgtagc taagcgcttc ttttaccgac
    54781 ttcggctgat gcagcgccat ctcttcggcg gtcacaacag aaatagactg tggcactttt
    54841 tgaatcggcg tatcggtttt agtgccggta gctgactgtc gcgccgcaat agttgcagca
    54901 ggcccccatg cgctttcttg cggcgcaggt gcagcggtaa cggtgatagt gtcttctttc
    54961 ggttcaaccg ctgcctgtgc ataaacagac atgccgctaa ccgctgtggc tactacaact
    55021 gcgattttac gcagtgagtg ttttggctga gcagttttgg aacgcgccat tggtatatct
    55081 ctgatgtaaa gtgaatgata acgtaaacga gaatgattat tataaagata agataatatt
    55141 cgaaactctg gcgggttcgc aagcggtacg caatctgagc gttcctgaag ggttaataaa
    55201 caaccagatg aaaagaaagg gttaatactt tgggtgggat gttattttac cggatggcaa
    55261 ctcgccatcc ggtatttcac gcttagatgt taattactac caaacatatc cttaatccaa
    55321 ccggctacac cgtcgctgtc tttctgctct tgctgagcag gttgctgttg cggctgttgc
    55381 tgcggctgag aagactgatc aaacggattg cctgacggct gctgctgcat ctcactctgc
    55441 tggcacagcg attgcggatc gctggtccag accggcaaga cacgcatgcc accgctgcaa
    55501 acaaagttgc cgtcgtagtc cacgcccata tctgcaatat cttctggcgg aacaagattc
    55561 agcggcgttg gcgtctggtt agccagataa cgctgataaa tcgacattgc cccgctggct
    55621 ccatacagtt tggtcggctg gttgttatca cggccgaccc aggtgatggt caccgtgctg
    55681 ccgtcaatgc ccgcaaacca ggtatctacg ttattgttgg tagtccctgt tttccctgcc
    55741 agatgcaggt tcgggtattt cgccccaagc tgacgacccg taccgcgttg taccacctgc
    55801 tgcatggtcc atagtgtcag atacgccgcc tgcgccggaa cagcgcgttc cgcctgcggg
    55861 aagctctgat acagcacttt gccatcttcc gcgattaccg aacgcagcgc agaaagcggt
    55921 gcacggttac caccgctggc gatggtctgg aatgcctgcg ccacttcgat tggcgttaag
    55981 ttcaacgccc ccagcagcat tgccggaacc ggatgcaact gatctttcgg tacgcccagt
    56041 ttaatccagg tatccgtaac cgcaggcagc cccagcgcca tccccagatt taccgtcggc
    56101 acgttcatcg aacgggtcaa cgcatccacc agcatcactc tgccgctttc gctataacga
    56161 cggtcatcat tctgcggtga ccagacctgg ccattcggct gacgcagcgc aattggcgca
    56221 tccgcaatcc acgtattcag acgatagatt ttcggctggc ttaaggccgt cagataagtc
    56281 gctggttttg caagggaacc aatcgaacga cgcgcctgca tcgcacggtt gtagcccgca
    56341 aactgcggct cagaacctcc gaccatcgca cgaacttcac cactaaagcg gtcgacgacc
    56401 acaatcgcag tttcaagatc gctcaactta cgctgtttct tcagtgccgg aataccttcc
    56461 acggcggctt tttctgccgc gtcctgggcc accgagtcaa aggtagtgaa gatcttcacg
    56521 ccggagagat cttttacctt atcgcctaat ttagcctgaa gctcctgacg caccagttgc
    56581 ataaaggctg gctgaggaga gatcacccca ccgcgcggct gaacccccag cggacgcgca
    56641 ctcaacatgt catagagttc ttgatcaata atctgttgct gttgcagcag acgcagtacc
    56701 agattacgtc gctccagcgc cagtttcggg ttacgccacg ggttgtagat ggacgctcct
    56761 ttcaccatac cgactaacag cgcctgctga tcgaggctca attcttctac cgggcgacca
    56821 aagtaataca agctcgccag cgggaagccg cggatttcgt tgtcgccgct ctgaccgaga
    56881 tacacctcgt tcatatacag ctcaagaata cggtctttgc tgtaacgcgc gtccatgatc
    56941 agcgccatgt aggcttcatt cgctttacgc caataagagc gctcgctgga gaggaacagg
    57001 tttttcacca gctgttgcgt cagcgtactc gccccctgca ccgtgcgacc tgcggtcagg
    57061 tttgccagca ccgcacgtcc gatggagtag agactgattc catcatgctc gtagaaatgg
    57121 cggtcttctg tcgccagcaa agtatccacc agcaaatccg ggaaaccact gcgcggcaca
    57181 aacaaacgct gctcaccgtt tggtgaggag atcatggtga tcagacgcgg atcaagacgg
    57241 aagaaaccga actgacggtt gttctccata ttgacgatgg tcgccagatg atcgccatca
    57301 aaggtcagac gcgcgcgcac ctgcccttct ttactgtccg ggaaatcaaa cggacggcga
    57361 atcatctcaa tgctgttggc ctgcacggta aattcgccag gacgggtcat tttcgacacc
    57421 tgacgatact gtgttgcctc cagcagcttc accatctcgt tcttgctgat ggtcatgtct
    57481 ggctcaaggt tgaccattcg accataaact gccgcaggca gttgccagac cttgccatca
    57541 atacggctac gaattttttg atcgagataa acgccgtaaa tggcgatcag cacggcaaaa
    57601 acgatagcca gttttaacag cagccatagc cagccgcgtt tgccacgagg cttacgccct
    57661 ttgcctttgc ccttaccttt gcgcggcatc ggttcttcat cctcatagtc atcataatcg
    57721 tcgtaatcat cgtcatcttc gtaacgacga cggcttacct tttgtttgac cggacgcgtc
    57781 ggtttccctt tgcgtccaat tggctcgcgg tcattcccgg ccatgctttt tctccgcaat
    57841 attcaggcgc aaaagcccga ttttctgttc ttctgttaca agacagaaga agatatctct
    57901 caaaatttga gctcgatcgg tcccctctcc cttaagggag agggttaggg tgagggtgaa
    57961 cgtgcgcgct ggtgccctca ccccggccct ctcccacagg gagagggaga aaaccaacct
    58021 tacgaatact ttttcgtccg tcgcgtcggt gccgtgtttg ccgggtcgtc cggccagacg
    58081 tgtttgggat aacgcccttt catctccttt tgcacctcac gatacgcccc tttccagaag
    58141 gcccccaaat cacgcgtgat ttgcagcggc ctttgggcgg gtgaaagcaa ttccagcacc
    58201 agcggcacgc gcccctgggc gatcgtcgga ttagtggcct caccaaacat ctcctgcatt
    58261 ctcaccgcca gcgcgggcgg gttatcttca tgataacgaa tggcgatccg gcttcccgtc
    58321 ggcacagtgt aatgcgcagg caattcacta tccagacgtt gctgcattcc ccaatcaagt
    58381 aatccgcgta gtgcctgata aatgtcgagt gatttcaggc cgcgtagtga atgtacaccg
    58441 gccatatgtg gcagcagcca cgtttccagc gtcgccaaca aactttcatc atcaaccgct
    58501 ggccagtcat attctggcaa ccactttccg gcgcataaca aacgcaagcg tagctgttcc
    58561 gcttccgccg tccagttgag cacgcttaaa cctttatcac ggatgccgtt aagcatcgcc
    58621 tgatgcaact cgtcttctga cggtttcgcc aacggctgca ctttcaccgt caactgaccg
    58681 atttgcagcc gacgccaggc tttcagcgta ccttgcgcgt catcccattc cacggtgtca
    58741 gattgctgca ccagctgcgg acagcgttgt actaattcat caatatcgac cggtaacgcc
    58801 agtaaaatcc gcgcatccgg cgaggcgctg ccctgcaata ataacggcgc gatcaaccat
    58861 tcgtggcgac ttagcgcgtc gtcggcatca agcatcgccc ccatgccgtt tgccagttga
    58921 tagcgtccat cttgcccacg gcggcgagca atgcgatcgg caaacgcccc ggcaagtagc
    58981 ggcgcgataa gcgaactgtc tgcctcaccg ccgcgtacgt ttaagcgttt taacagttgc
    59041 tgactacgtt gctgccaggc tggttgattg cgcgaaaacg ccacgcccag gtcactattg
    59101 cccatccgtg gcggctcttc gagaatggcg gcaatttttg ccgcggtagc agcttcgtcg
    59161 tcgctctttg cgctaaccag cattgccgct aaacgcggat cgttacccag cgctgccatt
    59221 ttttgccctt gcgcactaag ccgttcaccg tccagcgccc ccagcatttg taacagacgt
    59281 ttcgcggcca gtagattcac cgttggcggt tgatccagcc agctcatctg cgccggatcg
    59341 ctgcatcccc attgcagtaa ttccatcagc aaaccggaaa gatcgctttg taagatctcc
    59401 ggttcacttt gcgcggtagc gcgttctgct tgttctttgg cgattaaatg caggcagata
    59461 cccggctcca gacgcccggc gcgcccggca cgctgcgtca tcgacgcctg actgattcgc
    59521 tgagtaatca accgcgtaag ccctgttcgc ggatcaaaac gcgccaccct ttcctgggca
    59581 caatccacca ccagacgaat accttcaatg gttaaactgg tttcagcaat attggtcgcc
    59641 agcaccactt tgcgcatccc ttgcggtgcc gggaggatcg cttttcgctg atcgttcagc
    59701 gacaacgcgc catacagcgg gcagagcaat acatcactgc cgatgcgcga agccagttgt
    59761 tcctgcacac gctgaatttc tccgacgcca ggtaaaaata acagtaatga tccgctttcc
    59821 tgacgcagca tttcagcggt ggctaccgca acggcatcgt caaaacgctg atgcgtgggc
    59881 agcggtaaat aacggcgttc aaccggaaac gagcgccctt ctgagatgac gacgggcgct
    59941 tctggcagca tttgctgcaa gcggtcgttg tccagcgtag ccgacataat cagcagttta
    60001 aggtcatcac gcagaccttg ttgcacatcg agtaacagcg ccaacgccaa atccgcctgc
    60061 aagctacgct catgaaattc atcaaggatc accaatccaa caccgctcag ttccgggtca
    60121 cgctggatca tccgcgtcag cacgccttcg gtaactactt ccaggcgggt attcggcccg
    60181 acgcagtttt gcgcacgcat ccggtagcca acggtatcgc ctggcttttc gttaagcagc
    60241 tccgccagcc gttgcgcgac gttacgcgcc gccagacgcc gtggctccag gaggataatt
    60301 ttcccgttaa tgccgggatg cgccagcagt tgcagcggca gccaggttga tttcccggcc
    60361 ccggtcggcg cacttaataa tacctgcggc gcataatcga gagcggtaag taattcaggt
    60421 aacacggcag caacgggcaa cgacgacaca aaacgctcca gagggttaac attcttcgcg
    60481 ccacattgta gcatcgcggt aattcataac cgagtgcctc acatgtctga cccgcaacgt
    60541 ctgttctttg ctatcgactt acctgcagaa atccgcgaac agattatcca ctggcgcgcc
    60601 acacacttcc cacttgaggc gggacgtccg gtcgccgccg ataatttgca tctgactctg
    60661 gcatttttag gcgaagtgag cgcagagaaa gagaaggcgc tttctctttt agccggacgg
    60721 attcgtcaac ctggtttcac actcacgctt gatgacgccg gccaatggct gcgttcgcgt
    60781 gtggtgtggt tagggatgcg tcagccgcca cgcggcttaa tccagctggc gaatatgctc
    60841 cgttcacagg ctgcccgcag cggttgtttt caaagcaatc gtccgtttca tccacatatt
    60901 accttattgc gcgacgccag cgaggcggtg acaatcccgc cgccaggttt taactggtca
    60961 tatgcggtga cggagttcac cctttacgcc tcctcgtttg cccgtggacg cacacgctac
    61021 acgccgctaa aacgctgggt gctaacgcaa taacaaggat tgtcgcaatg gaattttctc
    61081 cccctctaca gcgcgcgacg ctaattcagc gttacaaacg ttttttagcc gatgtgatca
    61141 cacccgatgg tcgcgaatta acgctacact gcccgaatac gggtgcgatg accggttgtg
    61201 caacgcctgg cgataccgtc tggtattcga cttcagacaa caccaaacgg aaatacccac
    61261 acacctggga attaactcaa agccagagcg gcgcatttat ttgcgtcaac acgctttggg
    61321 ctaacaggtt gacgaaagag gctatcctta atgaatcaat ttcagaactg tcaggctata
    61381 gctcgctgaa aagcgaagta aaatacggcg cagaacgcag ccgtattgac tttatgttgc
    61441 aggcggattc gcgtccagac tgctatattg aagtgaaatc ggttacgtta gcggagaacg
    61501 aacagggata ttttcccgat gcggtcactg aacgaggtca gaaacacctt cgggagttga
    61561 tgagcgtagc ggctgaaggc cagcgtgcgg ttatcttttt cgccgtgctg cattcagcca
    61621 ttacacggtt ttcacccgcg cgccacatcg atgagaaata cgcgcaacta ttgtcagaag
    61681 ctcaacagag gggggtagaa attctggctt acaaagcgga aatttctgct gaaggcatgg
    61741 ctcttaaaaa atcactgccg gttacattgt agtaaagtaa gtaactggtt aatttacatt
    61801 ctggtcgcgt gcgcaaatac gcttttcctc acacagttgt caagtgttac gtttagataa
    61861 ttgctatccg gaaaagcatc tgctatttat agcggcctca tttttccccc gaacatgggg
    61921 atcgatagtg cgtgttaagg agaagcaaca tgcaagaagg gcaaaaccgt aaaacatcgt
    61981 ccctgagtat tctcgccatc gctggggtgg aaccatatca ggagaagccg ggcgaagagt
    62041 atatgaatga agcccagctg gcgcacttcc gtcgtattct ggaagcatgg cgtaatcaac
    62101 tcagggatga agtcgatcgc accgttacac atatgcagga tgaagcagcc aatttcccgg
    62161 acccggtaga ccgtgcagcc caggaagaag agttcagcct cgaactgcgt aaccgcgatc
    62221 gcgagcgtaa gctgatcaaa aagatcgaga agacgctgaa aaaagtggaa gacgaagatt
    62281 tcggctactg tgaatcctgc ggtgttgaaa ttggtattcg ccgtctggaa gcgcgcccga
    62341 cagccgatct gtgcatcgac tgcaaaacgc tggctgaaat tcgcgaaaaa cagatggctg
    62401 gctaattaca gccgttccat cacgtttacc acaggcggga aatgctcccg ccttattttt
    62461 tgttcaaaga gatgacagac acacagtata ttggccgctt cgccccctct ccttccggcg
    62521 agcttcattt tggttctctg attgctgcgc ttggcagcta tttgcaggct cgcgcccggc
    62581 acggtcgctg gctggtacgc attgaagata tcgacccgcc tcgtgaagtt cccggtgccg
    62641 cagaaactat cctgcgccag ctggaacatt acggtctgca ctgggacggc gatgttctct
    62701 ggcaatcgca acgtcaccac gcctatcgtg aagcactcgc ctggttacat gaacaaggac
    62761 taagttatta ctgcacctgc acgcgtgcgc gtattcaaag cattggcggt atttacgacg
    62821 gtcattgccg ggtgttgcat catggaccag acaacgccgc agtgcgtatc cgccagcagc
    62881 atccggtcac gcaatttact gaccagctgc gcggcattat tcacgccgac gaaaaactgg
    62941 cacgggaaga ttttattatt catcgccgtg atggtttgtt tgcctataac ctggcggtgg
    63001 tggtggatga tcacttccag ggcgtgagcg aaattgtccg cggggcagat ttgatcgaac
    63061 cgacagtgcg gcaaatctcg ctgtaccagc tttttggctg gaaagtgcca gattacattc
    63121 atctgccgct ggcgcttaat ccacaaggcg ctaaactttc caagcagaat catgcgcctg
    63181 cgttgccgaa aggcgatcca cgcccggtac taatcgcggc acttcaattt ctggggcagc
    63241 aggcagaagc acactggcag gatttcagcg tcgagcaaat ccttcagtca gccgtcaaaa
    63301 actggcggct taccgcagtg cctgagtcgg caattgtaaa ttcaacattc tcaaatgcgt
    63361 catgctgagc tatgattagc cgctattttt ttgtcctgaa tgatgtttga cactaccgag
    63421 gtgtactatt tttacccgag tcgctaattt ttgccgcaag gtgctaagcc gcgaggaaag
    63481 cgaggctgaa caggcagtcg cccgtccaca ggtgacggtg atcccgcgtg agcagcatgc
    63541 tatttcccgc aaagatatca gtgaaaatgc cctgaaggta atgtacaggc tcaataaagc
    63601 gggatacgaa gcctggctgg ttggcggcgg cgtgcgcgac ctgttactcg gcaaaaagcc
    63661 gaaagatttt gacgtaacca ctaacgccac gcctgagcag gtgcgcaaac tgttccgtaa
    63721 ctgccgcctg gtgggtcgcc gtttccgtct ggctcatgtg atgtttggcc cggagattat
    63781 cgaagttgcg accttccgtg gacaccacga aggtaacgtc agcgaccgca cgacctccca
    63841 acgcgggcaa aacggcatgt tgctgcgcga caacattttc ggctccatcg aagaagacgc
    63901 ccagcgccgc gatttcacta tcaacagcct gtattacagc gtagcggatt ttaccgtccg
    63961 tgattacgtt ggcggtatga aggatctgaa ggacggtgtt atccgtctga ttggtaaccc
    64021 ggaaacgcgc taccgtgaag atccggtacg tatgctgcgc gcagtacgtt ttgccgccaa
    64081 attgggtatg cgcatcagcc cggaaaccgc agaaccgatc cctcgcctcg ctaccctgct
    64141 gaacgatatc ccaccggcac gcctgtttga agaatcgctt aaactgctac aagcgggcta
    64201 cggttacgaa acctataagc tgttgtgtga atatcatctg ttccagccgc tgttcccgac
    64261 cattacccgc tacttcacgg aaaatggcga cagcccgatg gagcggatca ttgaacaggt
    64321 gctgaagaat accgatacgc gtatccataa cgatatgcgc gtgaacccgg cgttcctgtt
    64381 tgccgccatg ttctggtacc cactgctgga gacggcacag aagatcgccc aggaaagcgg
    64441 cctgacctat cacgacgctt tcgcgctggc gatgaacgac gtgctggacg aagcctgccg
    64501 ttcactggca atcccgaaac gtctgacgac gttaacccgc gatatctggc agttgcagtt
    64561 gcgtatgtcc cgacgtcagg gtaaacgcgc atggaaattg ctggagcatc ctaagttccg
    64621 tgcggcttat gacctgttgg ccttgcgagc tgaagttgag cgtaacgctg aactgcagcg
    64681 tctggtgaaa tggtggggtg agttccaggt ttccgcgcca ccagaccaaa aagggatgct
    64741 caacgagctg gatgaagaac cgtcaccgcg tcgtcgtact cgtcgtccac gcaaacgcgc
    64801 accacgtcgt gagggtaccg catgacagtg gcgtatattg ccataggcag caatctggcc
    64861 tctccgctgg agcaggtcaa tgctgccctg aaagcattag gcgatatccc tgaaagccac
    64921 attcttaccg tttcttcgtt ttaccgcacc ccaccgctgg ggccgcaaga tcaacccgat
    64981 tacttaaacg cagccgtggc gctggaaaca acacttgcgc ctgaagaatt acttaatcac
    65041 acacagcaaa tagagttgca gcaaggtcgc gttcgtaaag cagaacgctg gggaccgcgc
    65101 acactcgatc ttgatatcat gttgtttggt aatgaaacca ttaataccga acgactcacc
    65161 gtcccgcatt acgatatgaa gaatcgcggc tttatgctgt ggccgctgtt tgagattgcc
    65221 ccggaactcg tgtttcctga tggggagaca ttacgtgaaa tactcacttc gctagcagta
    65281 ctacaacctg ccatctggta ttagctgtat tattttattt tcttatctta ctcctcactt
    65341 cccctcttac agattaaata tacttaattt acattttagt tttttttaat taattcatga
    65401 aatagcactt catgcaaata gattaggcat tttttgttta aaaaacctag aatagcaaca
    65461 attcaataca ccacgtaaat atattcacac ccgtgataat tcacccctat tacatcctcc
    65521 aaaaactttg agtaatatcc gctcgcaata ttaatttcat ataatttttg taaggatgca
    65581 tgtaatgaaa aaagcacttc tcgcagccgc tctggttatg gcttctggtt ccgccctggc
    65641 tgtagatggt ggtcatatcg actttaacgg tatggtacag tccggtacct gtaaagtggg
    65701 tgtggtagat actggtatgc atagcgttac cactgatggc gtggttaccc tggatactgc
    65761 gaatgttact gatacttttg ctgaagttag cgcaactgct gtcggtttac tgccgaaaga
    65821 gttcatgatt tctgttgagt gtgatccagg tgctccgaag aatgctgagt taactatggg
    65881 ttctgcaagt tacgcgaaca ccagcggtac cctgaataac aatatgaaca tcactgttaa
    65941 cggtattgca ccggctcaga acgtaaacat tgcagttcat aacatgaaaa acaaagctgg
    66001 cgctgctgaa attaagcagg tccatatgaa caactcttct gaagttcagg aactgacatt
    66061 agacgcagaa ggtaaaggcc agtacgtatt taacgcatct tacgttaaag caccgaacag
    66121 cccggctgta actgctggtc atgtaaccac taacgcgctg tacaccgttg cttataagta
    66181 attgatggtg tgttgagcat aaggtttgcc agcaggcaaa ccttattttc gcgaatttaa
    66241 tctttctaca gacagcaagg agtgcttatg tttcccttgg ttaaaaaaac ggttagtgca
    66301 ttatttgtga gtactctgtt agccagtgca ccagcattcg ctgacattat tatttccgga
    66361 acacgtatca tttataacgc tgataaaaaa gatgttaacg ttcgtctgga aaataaaggg
    66421 aatcgcccct tactgattca gaactggttg gatacgggcg atgacaatgc cgatcccagc
    66481 cagatcaaag tgccgtttac cgcgacccca ccagtttcac gcgtggagcc aaaacgcggt
    66541 caaaccgtaa aagtgatgta taccggtgca acagccctgc cgaaagaccg tgaaagcgtc
    66601 tactggttta acgtgctgga agtgccgcct aagccgaaag atgctgaggc tgacaaaaac
    66661 ttgttacagc tggctttccg cacacgcatc aaactctttt atcgcccgag tgggttacag
    66721 ggagaaccgg ctgaagcacc ggcgaaaatc acctggaaat taaacaacgc tcagttacag
    66781 gcaaataacc caacgccata ttacgtgtcc tttaatgagg tcaagctgga aagcggcggc
    66841 aaaacctaca acgtcaattc cagcatggtc acgccgttca gccaggcaag ctttggcgta
    66901 accagtctgc caggaagcgt gtcatccgga aaagtggtct ttaaagcgat caacgacttt
    66961 ggcggcaaca ttgatggttc cgcaacattc taaaaaataa acggattctt tttaacatcg
    67021 ccaggaatgc aaccttgtac cagtttactc atcaaaaaag ccgtatcccg aaaaaaacgc
    67081 tacttgcggc ctgttgtgcc ctgttttata gcagcaacgg tgctgcggcg gacaccgtgg
    67141 aatatgacag ttccttttta atgggaactg gcgcatcaac gattgatgtt aaacgttatg
    67201 ctcaaggcaa cccgacaccg ccgggtctct ataatgtccg cgtatttgta aacggtcagg
    67261 cgacttccag cttagaaatt ccgtttgtgg atattggcga aaacagtgcg gcggcctgtc
    67321 ttacccataa aaacctggcg caacttcaca ttaagcaacc tgaacagcct gtcactttac
    67381 tcgccagaga aggtgaagaa gaggattgtc tggatctggc aaagtcatac gaaaaggcgg
    67441 atgtgtgctt tgacggtagt gaccagtttc tcgatctgac gatccctcag gcctatgttc
    67501 tgaaaagcta tggcggctac gttgaccctt ctttatggga atcgggaatt aacgctgcca
    67561 cactggcata taccctgaac gcgtatcaca caagttcaga taacgacaat agtgacagcg
    67621 tctatggcgc gttcaactca ggtatcaatt taggagcctg gcactttcgt gcgcgcggta
    67681 actataactg gacaacagat aacggcagcg atttcgattt ccaggatcgt tacttacagc
    67741 gtgacattcc ggcaatccgt tcccagataa ttatgggtga tgcctatacc accggtgaaa
    67801 cgtttgactc tgtcaacgtc cgtggtgttc gcctgtacag cgacagccgt atgctgcctt
    67861 cggcgctggc cagttacgct ccgaccatcc gcggtgtagc aaactccaac gccaaagtca
    67921 ccgtgacgca aagcggatat aaaatttatg aaaccaccgt tccgcccggt gaatttgtta
    67981 tagacgacat tagcccttcc ggctttggta gcgaactggt cgtgaccatt gaagaagcgg
    68041 atggttccaa acgcaccttt acgcaaccct tctcgtcggt tgtacaaatg caacgtcctg
    68101 gtgtgggccg ttgggatttc agcgcgggta aagtcattga tgacagtctg cgatccgaac
    68161 ccaatatggg gcaagcctct tattactatg gtctgaataa cctcttcacg ggttataccg
    68221 gcattcagtt caccgataat aactatcttg ccgggctgtt aggtgtgggt atcaacacca
    68281 gcatcggcgc ctttgcggta gacgttaccc attcccgtgc tgaaattccg gatgataaaa
    68341 cctaccaggg gcaaagttat cgcgtgacct ggaacaaact tttccaggat accgggacat
    68401 catttaacct cgcggcgtac cgctattcca cccaggatta cctgggcctg catgatgcgt
    68461 tagtcctcat tgacgacgcc aagcatttgt ctgccgatga agacaaaaac accatgcaga
    68521 cgtactcacg tatgaaaaac cagtttaccg tcagcattaa ccagccattg aatatcgcct
    68581 atgaagatta cggttcgctg tttatttccg gtagctggac gtattactgg gcggcgaaca
    68641 atagccgcac tgaatataat gttggttaca gtaaaagcgt ttcgtggggc agtttcagcg
    68701 tcaacctaca acgtagctgg aatgaagacg gcgagaaaga tgacgcgatg tacgtcagcg
    68761 ttagcgtacc tattgagaat attttaggtg gcaaacgtaa gtcttctggt ttccgcaatt
    68821 taaatactca gctcaatacc gatttcgatg gttcacatca gttgaatgtt aacagttccg
    68881 gtaacactga aaacaatctg gtgaactaca gtgtcaacgc aggttatagc ctcgataaaa
    68941 acgccggcga tttagcctct gttggtggtt atctcaacta tgaatctggg ttaggcggta
    69001 tttccgcttc ggcctcggcc acttctgata acagccaaca gtactccatc tcaaccgatg
    69061 gcggctttgt attacacagt ggtggtttaa cgttcactaa caacagtttc agcagtaacg
    69121 acacgctggt gttaatcaac gccctaggtg ctaaaggcgc acgaatcaat aacagtaata
    69181 acgaaatcga tcgctgggga tatgccgtga cgtcctctgt cagcccatat cgtgaaaacc
    69241 gggtaggtct gaacattgaa acactggaaa acgatgttga actgaaaagt accagcgcca
    69301 ccaccgtacc acgtagcggc tccgttgttt tgacccgttt cgaaactgac gaggggcgtt
    69361 ctgccgtgct gaatattact gccgccaatg gcaaatccat tccgtttgct gcggaggttt
    69421 accagggtga ggtgatgatc ggcagcatgg gccagggtgg tcaggcattt gtacgcggta
    69481 ttaacgacag cggggaatta atcgtgcgct ggtatgaaaa caaccaaacc attgactgta
    69541 agttgcacta ccagttcccg gcgcagccac aaacgcaggg aagcaccaac accttattac
    69601 ttaacaatct tacctgtcag gtagcaaatc actaatatgg aaaattctat gaaaaggata
    69661 ctactaacat ccgcgttaat aggcctgggt ttaccggcgg ttggctctgc aacagatctt
    69721 aatgtcgatt ttacggccac cgtattagcc accacctgca cgataactat agtggaagat
    69781 ggtggtccag cagtgaccgg tagtaacgat gaatacagcc tgacaattcc tgatgttggt
    69841 ctggataaag tcgctaccgc tgcgcccgaa gctcaggcca actttaaatt aaaggcctcc
    69901 gattgcagca atggttactc aaagattttt accacactta caggaacaac ggtttccggt
    69961 aagttaattg ttaatgaagc aacctctggc gctgctgggg tggggatggg gattaagcgt
    70021 agagataccg cagactcgac tttcttcaca cccaataata ctgacaaatt tgaatggagc
    70081 gccgatgaga aagccagcgg tgtgcctctg accgtagcac tgcgcgaaac aacggcggga
    70141 gcaggacgca ccggtgcgtt tcaggcaaaa gccaccttta acttcaccta cgaataacag
    70201 ggacagccac catgaaatta aaagtcatcg ctacactgat tgctactgtt gccgtgggtg
    70261 taagctttaa cagcaatttt gcttctgcga gtacaacgtc cgcttcttta accgtaaaca
    70321 gtaacctgac tatgggtacc tgcagtgctc agataatgga taatagtaat aaagtgatca
    70381 atgaagtggt ctttggcaat gtttatattt ctgaactcgg tgcaaaaagc aaagtgcaac
    70441 agtttaaaat tcgctttagc aattgctctg gccttcccca aaacagcgcc caaatagtgc
    70501 tggcacctaa tggtatatcc tgtgctggtt ctcaatcgtc atcggcgggt ttttctaaca
    70561 agtttactga cgctagcgca gcaaccagaa cggctgtgga agtatggact acagatacac
    70621 cggaaagcaa tggcagtacg caattccatt gtgctcaaaa gataccagtg cctgtgacgc
    70681 ttcccgccga caccacaact cagccttacg attacccgtt aagtgcacgg atgaccgttg
    70741 cggaaggtag attggtaacc gatgtaagac cgggtaattt ccgctctccc acgactttca
    70801 cgatcactta tcagtaatag cggataagga tattagaatg ctatgcaggc atcataaaat
    70861 cgtacacttt ttagggctgg caacagccct tatcacgcct tttgcctatt ccggccagga
    70921 tgtggatctc accgcaaaga tcgtgcccag cacctgtcag gttgaagtca gtaataatgg
    70981 cgttgtcgat ctcggcacag tgacgctgga ctattttgcc gacaatgtaa ccccgacaac
    71041 ggattatgcg ggtggcaaaa cgtttaatgt gaatgtggtc tcttgcgata acattcaaac
    71101 gacacagtcg caaatgaaat tagattttca gccgcaggcg ggatcgcttg cacaggcgaa
    71161 taatcaaatt tttagtaatg aatatgaaca gcaggctacc ggggcgaaaa acgtcggcat
    71221 tgtgattttt tcggcccaac cgaatcagca gacatttaac gtgcgaggaa cggatggttc
    71281 ctcaacagca atttattcgg tagcacctgg caatgctgtt ccctcaacct ggacctttta
    71341 ttcccgtatg cagcgggtga ataatgccct tcctcctgaa tcgggaatgg tgagaagcca
    71401 ggttattgtt aacgtatctt acgaataata aggataatta tgcaaaggaa aggcaataaa
    71461 ctgttgattc agttatgcag tgtgatactg ctatttttta ccacatcctg gtatgcattg
    71521 gcgaatgaat gttatataga gagaaatgct gaaggggatt atcacatgaa gataagctct
    71581 actcagctta gtctggcgtc acaaatggtc gaggttccga cagaaatagc cgaagctaca
    71641 tgggatgtaa atattcaact aagaggcgat gccatagggt gtaaatctct tggggatagt
    71701 aaggcagttc actttcttaa tacagctgac ccaagtttaa tatccacgta caccacaacg
    71761 aatggcgcag cgttattaaa aacaactgtt ccaggcattg tgtattctgt cgagttatta
    71821 tgccttagtt gtggtgccgc agatgaactt gatttatggc tacctgcaca aagtggcgca
    71881 gataacttca taccaagcac ccagacgaaa tgggcctatg agtacagtga tcaaagttgg
    71941 tatttacgtt ttcgcttatt cataactcct gaatttaaac ccaagaatgg tgtttccagc
    72001 ggaacaacga tagcaggaaa gattgcgtca tggtatatag gtaccaatga ccagccgtgg
    72061 atcaactttt acattgacaa tgactcttta aagtttttcg tcgatgaacc gacctgtgca
    72121 acagttgccc tggcacaaga tcagggcaac gtcagtggca atcaggtaac gcttgggaac
    72181 agctatgttt cggaagtgaa aaatgggctt acgcgggaaa tccctttttc tatccgtgct
    72241 gaatactgtt atgccagtaa aattacggtt aagttgaaag cggcaaataa acccagcgat
    72301 gccacactgg tgggtaaaac gactggctcg gcttcaggcg tggctgtaaa agtaaattca
    72361 acttatgaca atagcaaagt attgttaaaa gcagatggta gcaacacggt tgactacaac
    72421 ttcgccgcct ggtcaaacaa cctgctgttt ttacctttta cggcgcagct ggtaccggat
    72481 ggtagcggta atgctgtcgg tgttggaaca ttttcaggta acgcgacctt ctcctttacc
    72541 tacgaataag taaaacgtca ttccctcctc atcgcaccag agttctgctc tggtgcgaac
    72601 atttccccac actaataacc attgcctccc tgttttcggc tgttagaatg cgatcaatct
    72661 gactttagcc atcaggatac gttatgaaac cgaccaccat ttcttcattg cagaaatgta
    72721 agcaggaaaa aaaacgcttc gccaccatca ccgcctacga ctacagcttc gcaaaactgt
    72781 ttgccgagga agggctgaac gtgatgctgg tgggcgactc gctggggatg accgttcagg
    72841 ggcatgaatc tacccttccc gttaccgttg aagatatcgc ctaccacact accgcagtac
    72901 gtcgtggcgc gccaaactgc ctgctgttgg ctgacctgcc gtttatggcg tatgccacgc
    72961 cggaacaagc ctttgaaaac gccgcaacgg ttatgcgtgc cggtgctaac atggtcaaaa
    73021 ttgaaggcgg tgagtggctg gtcgaaaccg taaaaatgct gaccgaacgt gccgttcctg
    73081 tatgtggtca cttaggttta accccacagt cagtgaatat tttcggtggc tacaaagttc
    73141 aggggcgcgg cgatgaagcg agcgatcgac tgcttagcga tgcattagcc ttagaagccg
    73201 ctggtgcaca gttgctggtg ctggaatgcg tgccggttga actggcaaaa cgtattaccg
    73261 aagcgctggc gatcccggtt atcggtattg gcgcaggcaa cgttaccgat gggcagatcc
    73321 tcgtgatgca cgatgcgttc ggcattaccg gcggtcatat ccctaaattt gccaaaaatt
    73381 tcctcgctga aacgggcgac atccgcgctg cggtgcggca gtatatggct gaagtggagt
    73441 ccggcgttta tccgggcgaa gaacacagtt tccattaagg agtgacgttg tgttaattat
    73501 cgaaaccctg ccactgctgc gccagcaaat tcgccgcctg cgtatggaag gcaagcgcgt
    73561 ggcgctggta cctaccatgg gcaacctgca tgatggccac atgaagctgg tcgacgaagc
    73621 caaagcccgc gccgatgtgg tcgtcgtcag tattttcgtt aacccgatgc agttcgaccg
    73681 cccggaggac ctggctcggt acccacgcac cttgcaggaa gactgcgaaa agctgaacaa
    73741 gcgcaaagtg gatctggttt tcgcgccctc ggtaaaagag atctacccga acggtactga
    73801 aacccatact tacgttgacg ttccagggct ttcgaccatg ctggaaggcg ccagccggcc
    73861 ggggcatttt cgcggcgtgt cgactatcgt cagcaagctg tttaacctgg tccagccgga
    73921 catcgcctgc ttcggtgaaa aggactttca gcaactggcg ctgatccgca aaatggttgc
    73981 tgatatgggc tttgatattg agattgtcgg tgtgccaatt atgcgcgcca aagacggtct
    74041 ggcactgagt tcccgtaacg gttatctgac ggcagaacaa cgcaaaattg cgcccggtct
    74101 gtacaaagtt ttaagttcga ttgccgacaa attgcaggct ggcgaacggg atctcgatga
    74161 aattattgct attgccgggc aagaactgaa tgaaaaaggc ttccgcgccg atgatattca
    74221 gattcgcgat gccgacacat tgctggaagt ctctgaaacc agcaaacggg cagtaattct
    74281 ggtagccgcc tggcttggcg atgctcgcct gatcgacaac aaaatggtcg agctggcgta
    74341 atacttatac tctaaataat tcaagttgca agttgcagga aggcggcaag cgagtgacaa
    74401 attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga catggatgtc
    74461 tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa cgcagacgca
    74521 gcacatgcaa tttgaagtat gacgagtata actggcgcta cggctgatgg cgccaattat
    74581 taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac gcagacaact
    74641 tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc aacgtcttcc
    74701 cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctctcctc ctgtcttccc
    74761 tgctgaatcc ccttctcaat ccctttctct tcaaaccact gtgccagcgt catcatagac
    74821 ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg ttcagtatga
    74881 ccgcgttgca gcatatagtt ttgcatggca actaactgac tttcgctagt gtacccttcg
    74941 tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg aatatgtttt
    75001 tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc cggtgtgatg
    75061 gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc cggcgagtaa
    75121 aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg ataaaagaga
    75181 atcggcacta ccagcggtag tttgtcgtga tccgcctcca gatgccgatg catggcggct
    75241 atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg gtgttcaatc
    75301 acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac gtccgtgctg
    75361 tgtcctttaa ggctctcttc aatgaaactc cccgactcta aatgaagcgt gttgaggtca
    75421 caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc cgtctccgca
    75481 tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc atccatcttc
    75541 cctgtcttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc tatcgtcagc
    75601 cacctcttta ttgtttaccg agcagcgttc agagaaatat caattcgtta caggcgatac
    75661 atggcacgct tcggcgcggt acgcaaactg ctgatgcaaa tgccgtcaga tgagtagaca
    75721 ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac cgcctgcttc
    75781 gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag ggcaaactcc
    75841 accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc attgaccagg
    75901 attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg aatgtcacca
    75961 acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga attatttctg
    76021 ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc gccagcttcg
    76081 ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat tttgaaggcg
    76141 acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga ttgttatccc
    76201 tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct taagatataa
    76261 agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat ggttgtcacc
    76321 gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa gccgccaaac
    76381 ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcgacg tgaacgtgca
    76441 aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg ataaccatct
    76501 acccgatgca aaaaatgggt atgtgactgg aaatcaaata catcgcgaat ttcgttaagc
    76561 tcagaaacgc tcataaattg cagcgatttt ggttcccact tctgcgggtg acgtttgatg
    76621 cgcgaggtaa caataaacgc cgtcgccttc atgccatatt gtttcaacac aggatacgca
    76681 tagcggctca ctgacttgag gccatcatca aaggtaatca ccaccgctcg cgcaggaaga
    76741 ttgatcttat tcttcacgta accttccagc tgcaccatgc tcagtgtcgc gtatcccctg
    76801 tcgcgcagcc aggccatctg gttattgaaa gcgcgtaccg aggtggtcgt cgaagtatgg
    76861 cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aggttagcac cggaaggcca
    76921 ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat acggatctga
    76981 taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg caaattgtcc
    77041 gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg cgtatctttc
    77101 caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc gtcttcaact
    77161 ttttgtcgcc cctgaaccgg ctcaagatga cctttatcga taaacccttt atcaaagcca
    77221 aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg tccggcccga
    77281 atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc attttcgatg
    77341 gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag catcagcagg
    77401 agaataacag cttgtttgta catgaaaaaa tcaccgaggg tgaaacaacg cttttattgt
    77461 agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt gaggacgaaa
    77521 ggcggatttt tttgttgctg gtgccaaata ctactcacct ccggcgcacc cagttcgaca
    77581 atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat ctgttcaatt
    77641 aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct taataatgaa
    77701 gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc ctcaccagag
    77761 tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagaact taatccgcgc
    77821 cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag cacatccagt
    77881 atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc cttcctcctt
    77941 agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt caaaactcct
    78001 ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt ccacgttgga
    78061 tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg ccaaaggtag
    78121 tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg atcatataaa
    78181 cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc agtgagtaaa
    78241 agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg ctgatgtcat
    78301 cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac accgccgtga
    78361 gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca aaaaacagtg
    78421 aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg ccgacatatc
    78481 cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata ttacgctgga
    78541 acttggcacc aaaaaatgat gacgcgacgt tggcgtaggc gttggtgatc accgacatca
    78601 tgatcagccc cggtacgatg aactgcatgt agctgaagcc atgcatatcg ccaatacgcg
    78661 aaccaatcag gttaccgaaa ataataaagt aaagggtcat ggtgatgact ggcggcacca
    78721 gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg ctttttagcg
    78781 ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca gtgaaacaaa
    78841 cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc cctgctcact
    78901 taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt ccagcgtcgc
    78961 ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt tcggtgcgag
    79021 atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg aggtattttc
    79081 caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg cttcttccag
    79141 gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca aaaagcccca
    79201 cattgagcgg cgaagttcaa tatccacgcc tgcagtcggc tcgtcgagaa tcagtagttt
    79261 aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc cagataacat
    79321 gcgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt tttcgctgcg
    79381 gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca caatttgctg
    79441 cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact gacgtttagc
    79501 gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg aggttttatt
    79561 taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg gcccgagaag
    79621 cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct gaacgccgcc
    79681 tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt tttacttacc
    79741 ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta tattagccaa
    79801 acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac actcatcagc
    79861 aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt tgagaaactg
    79921 gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg cgttcctgca
    79981 gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt tgctaacctg
    80041 gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga tgtactcgaa
    80101 gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc cgcagttgaa
    80161 aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa
    80221 catagctcat tgctcgacga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg
    80281 aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa
    80341 cgcgggcaga aagttaccat tcacggctgg gcttatggca ttcacgacgg cttgctgcgt
    80401 gatctggatg ttactgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt
    80461 tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca
    80521 tccggcaact tcacacttac tcgtccagca gaatcacttt gccgatatac ggcagatgac
    80581 ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac
    80641 cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa
    80701 tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc
    80761 ccgagtcgat gatatcttca acaatcagca catccttgcc acggatatct tcatccagat
    80821 ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca
    80881 taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg
    80941 agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa
    81001 tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta
    81061 cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt
    81121 tttggcaccg caacaaagcg caagacacgc agtataccag caaaacgatt cattcgcgga
    81181 atctgttaat aaagctcaaa ttgtgatgac gatcacacat gttaaaacct ataattaatt
    81241 gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct
    81301 cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac
    81361 tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc
    81421 ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgtgccgcg ctttggctat
    81481 acgccgccct gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct
    81541 gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt
    81601 ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtggcac
    81661 tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag gaaatcaacg
    81721 gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc gatcaggact
    81781 ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa caaattaacg
    81841 ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc
    81901 cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc gacacccttt
    81961 acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc aaagagaaat
    82021 ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc tgccgtggcg
    82081 tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc
    82141 gtcgtatcat tcttccggtc aatgatggtc gcctgattgc gattaacgct gaaaacggca
    82201 agctgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca
    82261 ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa accatcgtga
    82321 tggcaggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt
    82381 ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg
    82441 caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcgtgg gcaccagcgg
    82501 cctatgacgc gaagctggat ctggtgtatc tgccgatggg cgtgaccacg ccggatatct
    82561 ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg
    82621 ccacaaccgg gaaattggcg tggagctacc agaccgttca ccacgacctg tgggatatgg
    82681 atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccagtta
    82741 tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgtaat ggcgaactgg
    82801 tggttccggc accggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc
    82861 ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata
    82921 tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct
    82981 atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg ggtaacctgg
    83041 gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc
    83101 caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac ccgatggagc
    83161 agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac
    83221 cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag
    83281 catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta
    83341 ttggtacgcc gcaggacagt atgccgttcc cgatgcctgt accggtgccg ttcaatatgg
    83401 gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta
    83461 cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc
    83521 gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggt aagcagtatg
    83581 tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggtgac tatattgtgg
    83641 cttatgcgct gccggatgat gtgaagtaag acttgctcag attgctgaca acgtgcgcgt
    83701 tgttcatgcc ggatgcggcg tgaacgcctt atccagccta caaaattgtg caaaatcaat
    83761 ggattgcaca ggtaacgtag gcctgataag cgtagcgcat caggcagatt tgcgtttgtc
    83821 atcagtttaa tgcccggaga gatccgggca tattcccgaa tacggtcttt ttataccgta
    83881 aaccctaaca tcatccccgt atcttcatgc tccagcagat ggcagtgcgc catataagca
    83941 cgttctttcg gtgcgtcgtg attaaacttc accagcactt cactgacatt gccttctacc
    84001 ttaacggtat ctttccagcc cgcgcgatga gccgctggcg gtttgccatt ttctgacaag
    84061 atacggaact gcgtgccgtg aatatggaac ggatgcagca tcatgtcgcc cacgccagag
    84121 ataacccaac gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt catatcaaac
    84181 gcctgaccgt tgattttatt ggcatggtgg aaatcgaact tcccgccgtg gttcatatga
    84241 ttcatattgc cgtgccccat atggcccatc atctggctgt gatccattcc ggccatcgcc
    84301 tgatcgccat atttctccat tagcatctgc atccccatca tatcgagcat cgggtccata
    84361 gaaagttgca gcttgcgtac cgtcagccct tccagcgaag gtaacgcagg caggctactt
    84421 aatgtgtctg gcaaagcacc ggaagcacta atagcaatcg gctgaatccg cattaccgga
    84481 tgaggcttat caaacggcgc aatcgccatc cccatctggc tgaccggcag cgtcaccagg
    84541 tcaaagggtt tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc catcagcacc
    84601 ggcagctcgt tcaccttcac cggttcaggt agcagaccac cgtcgctggc aatcacataa
    84661 agcgggcgat tgtcgctggt ggcgaaattg agcgagcggg cattacagcc attgagcaaa
    84721 cgcaggcgca gccaaccacg cggggcagcg tgttgcgggt agattgcacc gttggtcagc
    84781 aacgtatcgc caaaccagcc cacggcggcg gtcatcacat ccagttgata atcaatctgc
    84841 ccgtcggcgc taaatttctt atcctgaacg atcaccggaa catcatcgat accccactgt
    84901 tttggcagca ttaatttcag gatctcgtca tcttcaatca ccaccagacc agccagcccc
    84961 atcgccacct gtcgcccggt cttgccatgt tgatgtggat ggaaccagca ggtagcggca
    85021 ggttgatcaa cgttcaacgt caccgagcgc ttgccacctg gcggaataat tccctgtggg
    85081 ccgccgtcga cttcacccgg tacttccagc ccgtgccagt gcaacgtcgt ctcttccgtc
    85141 agttggttat agatatcaac cgttaccgct ttgccgcgct gtaatttcac cgccggcccc
    85201 agcagattgc cgttatagcc ccaggtagtt gcagttttct cgccaaaggt agactgacct
    85261 gcgccaatag ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc agggatcggt
    85321 aacgttgggc gttccgccgc aaagactgca cggctccaca gtggcaaggc tgaagccaca
    85381 cccagtgcga cggaatattt caagaaatca cgacgttgca tagttatttc cttattctta
    85441 agcaggcgaa acaaaatcaa acgttgagca tagtccttcc ccttacggga aggtcaagcc
    85501 gcagacacaa tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta
    85561 tcttctttaa gcccctggta gaagcaatga agacgttttt cagaacagtg ttattcggca
    85621 gcctgatggc cgtttgcgca aacagttacg cgctcagtga gtctgaagcc gaagatatgg
    85681 ccgatttaac ggcagttttt gtctttctga agaacgattg tggttaccag aacttaccta
    85741 acgggcaaat tcgtcgcgca ctggtctttt tcgcccagca aaaccagtgg gatctcagta
    85801 attacgacac cttcgacatg aaagctctcg gtgaagacag ctaccgcgat ctcagcggca
    85861 ttggcattcc cgtcgctaaa aagtgcaagg ccctggctcg cgattcctta agcctgcttg
    85921 cctacgtcaa ataatccctg ataccttttt gtagaaatat tgaccgtgca tccgcggtca
    85981 atgttagcta ttatgttgcg cccttttttt acgggtgtta acaaaggagg tatcaaccca
    86041 tggccgaaaa aaaacagtgg catgaaacgc tacacgacca gtttgggcag tactttgcgg
    86101 tagataacgt tctgtatcat gaaaagaccg atcaccagga tctgatcatt tttgagaacg
    86161 ctgcatttgg tcgcgtaatg gcgctggatg gcgtagtaca aaccaccgag cgcgacgagt
    86221 ttatctatca tgagatgatg acccatgttc cgctactggc ccacggtcac gcgaaacatg
    86281 tgctgattat cggcggcggc gacggtgcca tgctgcgtga agtaacccga cataaaaacg
    86341 ttgagtcaat cacgatggtg gaaatcgatg cgggtgtcgt gtcgttctgc cgtcagtatc
    86401 tacccaacca taacgccggt agctacgacg atccgcgctt taagctggtg atcgacgatg
    86461 gcgtcaattt cgttaatcaa accagccaga cctttgatgt cattatctcc gactgcaccg
    86521 atcctatcgg tcccggcgaa agccttttca cttcggcatt ttatgaaggc tgcaaacgtt
    86581 gcctgaatcc tggcggtatc ttcgtcgcac aaaacggcgt ctgcttttta cagcaggaag
    86641 aagccatcga cagccatcgc aaactcagcc attacttcag cgacgttggc ttttatcagg
    86701 cggcgatccc gacctattac ggcggtatca tgacttttgc atgggcgaca gataacgacg
    86761 ccttacgcca tctctcaacc gaaattattc aggcgcgttt tctcgcctct ggcctgaaat
    86821 gccgttatta caatccggca gtccatacgg cagcttttgc cttaccccag tatctgcaag
    86881 acgcactggc ttcacagccg tcctaaggag aagataagaa attgaaaaaa ctgaaactgc
    86941 atggctttaa taatctgacc aaaagtctga gtttttgtat ttacgatatc tgctacgcca
    87001 aaactgccga agagcgcgac ggttatattg cttatatcga tgaactctat aatgccaacc
    87061 gtctgaccga aatcctgtca gaaacctgtt ccattatcgg ggctaatatt cttaacatcg
    87121 cccgccagga ttacgaacca cagggtgcca gcgtcactat tctggtgagt gaagaaccgg
    87181 ttgacccgaa actcatcgac aaaacagaac accccggccc actgccagaa acggtcgttg
    87241 cccatctcga taaaagtcat atttgcgtac atacctaccc ggaaagtcat cctgaaggcg
    87301 gtttatgtac cttccgcgcc gatattgaag tctctacctg cggcgtgatt tctccgctga
    87361 aggcgctgaa ttacctgatc caccagcttg agtccgatat cgtaaccatt gattatcgcg
    87421 tgcgcggttt tacccgcgac attaacggta tgaagcactt tatcgaccat gagattaatt
    87481 cgattcagaa ctttatgtct gacgatatga aggcgctgta tgacatggtg gatgtaaacg
    87541 tctatcagga aaatatcttc cataccaaga tgttgcttaa agagttcgac cttaagcact
    87601 acatgttcca caccaaaccg gaagatttaa ccgacagcga gcgccaggaa attaccgctg
    87661 cgctgtggaa agaaatgcgc gagatttatt acgggcgcaa tatgccagct gtttaacggc
    87721 tctggcggag ctcccaggct ccgccagatt tatttacttc tgctgcacga aattgcggta
    87781 agccgccacg acctgcagaa aatcctcaac gccgcatagc gacaggcttt cttcgtcgta
    87841 gtagttcatc ccctcttcca tttcatcgcc agcgaattcc agttgattgg cgcgaaccat
    87901 cacctcttcg ccgtccatcc atagcgtata ctcatgccct gcccgctgcc aggaacgttc
    87961 gctacctttc agtgcgtgcg ctgcttgttc cacttcatca agcaaggcca ggttttcttt
    88021 cacctcttca ttaaaccagt gcccgaccac ttcatgcccc atggacatac gcacctttac
    88081 cactccggta atatcgcgca gaaattcgta atccatagtg tgttcctctg ctcccggcaa
    88141 tgcaccgaaa tcgcattgcg ccattgatgt aattatcgca gccttaccgg agaaaaacag
    88201 cggaacggcg atggtttaga aataaaaaaa tgcccgggaa tctcattcac cgggcattgt
    88261 gtcgtttatg cgcagcgcgt gcgctgactt tttaaaccgc agtctggaaa atcaccccat
    88321 cggctttctc ggtgtactga gaaagctggt tgaagttcag ataacggtaa gtatcaacgg
    88381 ctgttttatc tacctgcgcc acgtaggtct ggtactcttc cggcgtcggc agtttgccaa
    88441 tcagcgccgc aacagcagcc agttccgcag aagccaggaa gacattcgcg ccagtaccca
    88501 gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt tgcaccgtcc gccacacgcg
    88561 cctggttacc catacacagg gaacagccag ggatctcgat acgcgcacca ctcttaccga
    88621 agacgctgta gtagccttct tcggtcaact gtgcggcgtc catacgggtt ggcggtgcca
    88681 cccacaggcg ggtcggcaac tgacctttgt gcgcatccag cagtttacct gcggcacgga
    88741 agtgaccgat gttagtcatg caagaaccga taaacacttc gtcgatcttc tcgccctgta
    88801 ccgcagacag cggacgtgcg tcgtccgggt cgttcggagc acagaggatt ggctctttaa
    88861 tatccgccag atcgatgtcg atcactgccg cgtattccgc atctgcatcg gcttccagca
    88921 gctcaggatt cgccagccat ttttccatgc cctgaatacg acgttccagg gtacgacgat
    88981 cgccgtaacc ttccgcgatc atccacttca gcaggacgat gttggagttc aggtattcga
    89041 tgattggttc tttgttcagc ttgatggtac aaccggcagc agaacgctcg gcggacgcat
    89101 cggtcagctc aaaggcctgc tcaactttca gatccggcag accttcaatt tctaggatgc
    89161 ggccagagaa gatgtttttc ttgcctttct tctcaacggt cagcagacct tgtttgatcg
    89221 catacagcgg aatggcgtgt accagatcgc gcagggtgat gcccggctgc attttgcctt
    89281 tgaagcgcac cagaacggat tccggcatat ccagcggcat tacgcctgtt gcggctgcaa
    89341 atgccaccag accagaaccc gccgggaaag agataccgat cgggaaacgg gtatgggagt
    89401 caccaccggt accgacggta tccggcagca gcatacggtt cagccaggag tggattacgc
    89461 cgtcacccgg acgcagcgac acaccgccac ggttcataat gaagtccggc agcgtgtggt
    89521 gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg gcagaaagac tgcatcacca
    89581 ggtcagccga gaagcccagg cacgccaggt ctttcagttc atcacgggtc atcgggcctg
    89641 tggtgtcctg agagcctaca gaagtcattt tcggttcaca gtacgcgccc ggacgaatgc
    89701 ctttcacgcc acaggcacgg cctaccattt tctgcgccag cgagaagccg cgatcgctct
    89761 cagcgacatc tttcgcctga cggaacacat cactgtgcgg cagaccaagt gcttcacgcg
    89821 ctttggtggt caggccacga ccgataatca gcggaatacg gccgccagca cgcacttcat
    89881 caatcagcac gtcggttttc agttcgaagg tcgccagcag ttcgccggtt tcgtggttac
    89941 gtacttcacc tttgtacggg taaacgtcaa ttacgtcgcc catgttcagg ttagagacat
    90001 ccacttcgat tggcagcgca cccgcatctt ccatcgtgtt aaagaagatg ggtgcaattt
    90061 taccgccgag gcacaaacca ccgccgcgtt tgttcggcac atgtggaata tcatcgccca
    90121 taaaccacag aacggagttc gtggcggatt tacgcgatga acccgtaccc acaacgtcac
    90181 cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc aatctgtttg atcgggccaa
    90241 caacacctgg ctggtctggc tcaatacctt cacgggcgtt tttcagcatc gccagcgcgt
    90301 gcaatgggat atccgggcgt gaccacgcat ccggtgccgg agaaaggtca tcggtgttgg
    90361 tttcgccagt gactttgaag acggtaacgg tcagtttttc agccagcgcc gggcgattca
    90421 ggaaccattc ggcatcagcc caggactgca taacctgctt cgcatattcg ttgcctgctt
    90481 tcgctttctc ttctacgtca tagaagttat cgaacatcag tagtgtgtga gaaagtgctt
    90541 tggcagcgat cggtgccagt ttggcatcat ccagcgcatc gatcagcgga tgaatgttgt
    90601 aaccaccctg catagtaccc agcagttcga tggctttttc cggagtcagc agtggggatt
    90661 tggcttcgcc tttggcgaca gcagccagga agcctgcttt aacataggcg gcttcatcga
    90721 cgccaggagg aacacggttg gttaacagat ctaacaggaa ttcttcttcg cccgcgggcg
    90781 ggtttttcag cagctctaca agcgcggcca tttggtttgc atccaggggt ttgggcgcaa
    90841 tcccctcagc ggcacgctca gctacgtgct tacggtattc ttctagcacg acggttctcc
    90901 tcgctctcat tgtcatagtg cggcaggcga ttctcttcac gctcctgtga gacagcagtt
    90961 tgtagggtaa atgcccagta ccgcaacggg cagaatagca ggattttgac gacgtgttaa
    91021 tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg ttaaggatgg tctagagatt
    91081 gtttccctcc gtaaaaactt cgttaattcg catggtgata atcacctttc tcaacgaatc
    91141 ccaagattag tcaaaattta aactaccgcc tctttatact cggattcaca gcacctgcgg
    91201 gtggcagttc gccgaccatt gcgatttcct tgagatccga attatgaaaa tgactttgcc
    91261 gtttaaaccc catgtgctgg cgctaatttg cagtgccggg ctttgtgccg cctctgccgg
    91321 gctatatata aaaagccgca cagtggaagc gcctgtggaa acgcaatcga cacaactggc
    91381 tgtgtctgac gctgccgcag ttacgcttcc tgcaacggtt tccgcacctc ccgtaacacc
    91441 cgccgtcgtc aaatccgcat tcagcactgc acaaatagat caatgggtcg cgcccgtcgc
    91501 gctgtatccc gacgccctac tttcgcaggt gctgatggca tcaacctatc cgacaaacgt
    91561 tgctcaagca gtgcaatggt cgcacgataa tccacttaaa caaggcgatg ctgctattca
    91621 ggcggtatct gaccagccgt gggacgccag cgttaaatca ctggtggcct ttccacaatt
    91681 gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac ctgggcgatg cttttctggc
    91741 ccagccgcag gacgtgatgg actcggtaca acgattgcgg caactggcac aacaaaccgg
    91801 ctcgctgaag tcatcaaccg aacagaaagt tattaccaca acgaagaaaa ctgtaccggt
    91861 aacacagaca gtcacggctc ccgtcatacc atccaatacc gtttcaactg ccaaccctgt
    91921 cattacagag cctgcaacaa ccgtcatttc cattgagccc ggcaatcctg atgtggtcta
    91981 tattcccaac tacaacccaa ccgtggttta cgggaactgg gccaatactg cgtatccgcc
    92041 ggtttatctg ccaccaccag ccggagaacc gtttgttgac agctttgtac gcggattcgg
    92101 ctatagcatg ggcgttgcta ccacgtacgc actattcagc agcatcgact gggatgacga
    92161 cgatcatgac catcatcatc atgacgatga taattatcat caccacgatg gcggtcatcg
    92221 tgacggtaat ggctggcaac ataacggcga caacatcaat atcgacgtca acaatttcaa
    92281 ccgtatcacc ggtgagcatc ttactgataa gaatatggca tggcggcaca atccaaacta
    92341 ccgtaatggt gtgccctatc atgatcagga tatggcaaag cggtttcatc aaactgatgt
    92401 caacggcgga atgagtgcca cgcagctacc tgctccaaca cgcgacagcc agcgtcaggc
    92461 ggcagcaagt cagtttcagc aacgaacaca cgccgccccc gtcattacac gagataccca
    92521 acgtcaggca gcggcacagc ggtttaatga agctgaacac tatgggagct atgacgactt
    92581 ccgcgacttc agccgtcgcc aaccactgac ccagcaacaa aaggacgccg ctcgtcagcg
    92641 ttatcagtca gcttctcctg agcagcgcca ggcagttcac gagaaaatgc agactaaccc
    92701 gcagaaccag cagcgaagag aggcagcgcg tgagcgcatt cagcccgcct cgcctgagca
    92761 gcgccaggca gtccgcgaga aaatgcagac taacccacag atccagcagc gaagagacgc
    92821 agcgcgtgag cgtattcagt cagcctcgcc tgagcagcgc caggtgttta aggaaaaagt
    92881 acagcagcgc ccactgaacc aacagcaacg tgataacgcc cgccagcgtg ttcaatcagc
    92941 atcacctgaa caacgtcagg tttttcggga gaaagttcag gagagccgcc cacaacgtct
    93001 aaacgacagt aaccatactg ccaggctgaa taacgagcaa cggtcagcag tacgcgaacg
    93061 tctctctgag cgcggagcaa ggcgactgga aaggtaaatt acaggcgtaa aaaaagcggc
    93121 gtggttagcc gcttttttaa ttgccggatg ttccggcaaa cgaaaaatta cttcttcttc
    93181 gctttcgggt tcggcaggtc agtaatgcta ccttcgaaca cttctgccgc caggcccaca
    93241 gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc
    93301 atttcaattg ccaggccgat ttcacccagc agctcgccgc cgttagtacc gacaatcgca
    93361 ccaccgatca cacggtgaga ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag
    93421 tcggaagcga tagcacgacc agaagcagcc cacgggaagg tggcggtttc atagctgatg
    93481 cctttctctt tcgcttcttt ctcagtcaga cctacccatg caacttctgg ttcggtatag
    93541 gcgatggacg ggataacttt cggatcgaag tagtgtttct taccggcgat aacttcagcg
    93601 gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg gctgaccgac gatatcgccg
    93661 atagcaaaga tgtgcggtac gttggtacgc agctgtttgt caacgcggat gaaaccacgg
    93721 tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt taccgttcgg cacacgacca
    93781 atcgctacca gcacggcgtc gtaacgctgc ggttcagctg gtgctttttt gccttccatc
    93841 gtcacataaa taccgtcttc tttcgcttca acggcggtaa ctttggtttc cagcatcagg
    93901 ttgaatttct tgctgatacg cttggtgaag actttaacga tgtctttgtc agctgccggg
    93961 ataacctggt cgaacatttc aaccacgtca atctgtgaac ccagcgcgtg gtaaacggtg
    94021 cccatttcca gaccgatgat accgccaccc attaccagca ggcgttctgg tacttctttc
    94081 agttccagcg cgtcagtaga gtcccagata cgcggatctt catgcggaat aaacggcagt
    94141 tgaatcgggc gagaacccgc tgcaatgatc gcgttgtcga agttgatcac ggttttaccg
    94201 ttctcacctt caacttccag ggtgttagcc ccggtaaatt tacccagacc gttgaccact
    94261 ttgactttgc ggcctttcgc cataccagcc agaccaccgg tcagctgatt gatcactttc
    94321 tctttccagg tacgaatctt gtcgatatcg gttttcggtt cgccgaagac gataccgtgt
    94381 tcagccagcg ctttggcttc ttcgataact tttgctacgt gcagcagtgc tttagaaggg
    94441 atacagccga cgttcaggca aacaccgcca agggtgttgt aacgttctac gattacggtt
    94501 tccagaccta aatcagcgca acggaaggca gcggagtaac ctgcggggcc tgccccaagt
    94561 accacgacct gagttttgat ttcagtactc atcatgacct ctatatattt atctccggcg
    94621 ggtctgacgt tttttttatg acgcccatta tccaccgggt cgttctatcc gtcggtattt
    94681 tacaaaattg ttaacaattt ttaaacaaca aacggcaacc gatttgtcta ttcgctaata
    94741 acctcaatac attcatgaga ttaccagaaa aaagccggcc gttgggccgg ctcttttact
    94801 tacatcacca gacggcgaat gtcagacagc gtgttgttaa tgatggtaat gaaacgggca
    94861 ccatcagcac cgtcgatcac gcggtggtcg aaggagagag aaatcggcag catcagacgc
    94921 ggcacgaact ctttaccatt ccacaccggc tccatcgcgg acttggaaac gccgaggata
    94981 gccacttccg gcgcgttcac aatcggcgcg aagtgggtag tacccaggcc gccgatgctg
    95041 gagatggtga agcaaccgcc ctgcatttcg cccgcagtca acttaccgtc acgcgctttc
    95101 ttagaaatag tcatcagctc gcgagacagt tcgatgatgc ctttcttgtt gacgtctttg
    95161 aataccggaa caaccagacc gttcggggta tccaccgcca caccgatgtt gatgtatttc
    95221 ttcagggtca gacgctgacc gtcttccgac agcgaactgt tgaagcgagg catctgctca
    95281 agcgctgcag caacggcttt catgatgaag acaaccgggg tgatcttcac atccagctta
    95341 cgtttcgccg cttcttcgtt ctgctgttta cggaacgctt ccagctcggt gatatcggtt
    95401 ttgtcgaagt gagtaacatg cgggatcatc acccagttac ggctcaggtt cgcaccagaa
    95461 attttctgga tacggcccag ttccacttct tcgatttcac caaacttgct gaagtccacc
    95521 ttcggccacg gcagcatgcc agggatacca ccgccagtcg ctgccggagc tgcttctgca
    95581 cgtttgatag cttctttcac gtaagcctga acgtcttcgc gcaggatacg acctttacgg
    95641 ccagtgccct tcactttcgc aaggttaacg ccaaactcgc gtgccagacg gcggatcagc
    95701 ggggtcgcgt gaacgtaagc gtcattttca gcaaattcag atttgccttc cgctttcgca
    95761 gccggtgctg ctgccggggc ttcagctttt gctgccggag ccggcgctgc tgcttcctgt
    95821 ttcgcaggag ctgccgcagg cgctgcgcct tcaacttcga agatcataat cagcgagcca
    95881 gttttcactt tatcgccaac gttgactttc agttccttca cgacgcctgc aaacggagcc
    95941 ggaacttcca tagaagcttt atcgccttct acggtaatca gtgactgttc agcggcaact
    96001 ttgtcgccca ctttcaccat cacttcggtc acttcaactt cgtcaccgcc gatatccgga
    96061 acgttaactt ctttcacgcc agccgctggt gcaggggccg ctgccggagc cgcttcctgt
    96121 ttagccgccg gagctgccgc gcctgcttca cccgccactt cgaagaccat aatcagcgag
    96181 ccggtagaca ctttgtcgcc ggtgttcact ttgatttctt tcacggtgcc tgcaaacgga
    96241 gccggaactt ccatagaagc tttatcgcct tctacggtga tcagcgactg ctcagcttca
    96301 actttgtcgc caactttcac caggatttcg gtcacttcaa cttcgtcgct gccgatatcc
    96361 ggaacgttaa cgtcttttgc cgcggcagcc tcggggactg ctgccggagc tgcttctttc
    96421 ttctcttctg cctgagcagg tgcagcgtct gctgcaccgt cggcggaatc gaaaatcata
    96481 atcagtgcgc cggtctgagt tttatcgcca acagagactt tgatctcttt aacgataccc
    96541 gcctgcggag acggaacttc catagaagct ttgtcgcctt ctacggtgat cagcgactgt
    96601 tcggcttcaa ctttgtcgcc cactttgacc aggatctcgg tgatttcaac ttcatcagcc
    96661 ccgatgtccg gtactttgat ttcgatagcc attattcttt tacctcttac gccagacgcg
    96721 ggttaacttt atctgcatcg atgttgaatt tggcgattgc gtcagcaacc actttcttat
    96781 cgatttcgcc acgtttagcc agttcgccca gcgccgcaac cacgacataa gaagcatcaa
    96841 cttcgaagtg gtgacgcagg ttctcacggc tgtcggaacg accgaagcca tcagtaccca
    96901 gtacgcggta gtcgtcagcc ggtacgtaag tacggacctg ctcagcgaac agtttcatat
    96961 agtcggtaga tgccacagcc ggagcgtcgt tcatcacctg agcgatatac ggaacgcgcg
    97021 gagtttccag cggatgcagc atgttccagc gttcacaatc ctgaccatca cgcgccagtt
    97081 cggtgaagga ggtcacgcta taaacgtcag aacctacgcc gtaatctttc gccaggatct
    97141 ctgccgcttc acggacgtga cgcaggatag aaccggagcc cagcagctga actttacctt
    97201 tgctaccttc aatggtttcg agtttgtaga tacctttacg gataccttcc tcagcacctt
    97261 ccggcattgc cggcatgtgg tagttttcgt tcagcgtggt gatgtagtag taaacgttct
    97321 cttgtttttc accgtacata cgctccagac cgtcatgcat aatgacagca acttcgtaag
    97381 cgtaagccgg gtcgtaagag atacagttcg gaatagtcag cgactgaatg tggctgtgac
    97441 catcttcgtg ctgcagacct tcaccgttca gggtggtacg accggaagta ccgccgatca
    97501 ggaagccacg cgcttgctgg tcgccagccg cccagcacag atcgccgata cgctggaagc
    97561 cgaacatcga gtaatagatg tagaacggaa tcatcggcag attgttggtg ctgtaagagg
    97621 tcgccgctgc cagccaggaa caacctgcgc ccagctcgtt gatcccttcc tgcagaatct
    97681 gacctttctc gtcttcttta tagtaagcaa cctgctcgcg gtcctgcggg gtgtactgct
    97741 gaccgttcgg gctgtaaata ccaatctgac ggaacagacc ttccataccg aaagtacgcg
    97801 cttcgtcggc gatgatcggt accagacgat ctttgatcga cttgttcttc agcatcacgt
    97861 tcagggcacg aacgaaagcg atagtggtag agatctcttt gctctgctct tccagcagcg
    97921 cgccgaagtc ttgcaggctt ggcagctcaa gcttctcggt gaagttcggc tgacggcttg
    97981 gcagataacc gtgcagtttc tgacgctgtg cgtgcagata ggtatgctct tcagaacctt
    98041 ccgggaaggt gatgtacggc agtttttcga tatctgcatc agacaccggc acattgaaac
    98101 ggtcgcggat gtgacgcaca ccgtccatgt tcattttctt aacctggtgc gcgatgtttt
    98161 taccttcagc cgcgtcgccc atgccgtaac ctttaatggt atgagcaagg attaccgtcg
    98221 ctttgccttt ggtttcctgc gctttcttga atgcagcgta gattttcttc ggatcgtgac
    98281 caccacggtt cagtgcccag atctgctcgt cagtccagtc tgcaaccagt gctgcggttt
    98341 caggatattt accgaagaag tgttcacgaa cgtacgcacc atctttcgat ttgaaggtct
    98401 ggtagtcgcc gtcaacggtt tcgttcatca gctggatcag tttaccgctg gtatctttac
    98461 gcagcagttc atcccaacgg ctaccccaca tcactttgat cacgttccag ccagcacctt
    98521 cgaagatacc ttccagttcg ttgatgatct tgccgttacc ggtgaccggg ccgtcaagac
    98581 gctgcaggtt acagttgata acgaagacca ggttatccag tttttcacgg gtagcgatgg
    98641 tgatcgcacc tttggattcc ggttcgtcca tttcaccgtc gccgaggaac gcgtaaacgg
    98701 tctgtttaga ggtatctttc aggccacggt gttccagata tttcaggaat ttagcctggt
    98761 aaatagcacc aatcggaccc agacccatag atacggtcgg gaactgccag aattccggca
    98821 tcaatttcgg gtgcggatag gaagagaggc cattgccgtg aacttcctga cggaagttat
    98881 ccagctgctc ttgagtcaga cgaccttcca ggaaagcacg agcgtacacg cccggggaga
    98941 tgtggccctg gaagtaaacc aggtcgccgc catcctgctc gttgcgtgca cggaagaagt
    99001 ggttaaagca cacatcataa atggttgcgg aagactggaa ggacgccatg tggccgccca
    99061 gttcgaggtc ttttttcgac gcacgcagaa ccgtcatgat ggcgttccag cggatagctg
    99121 aacgaatacg gcgttccagt tccagattac ccggatactc cggttgttct tcaacgggga
    99181 tggtgttgat gtagttgctg ataccggtgc ctgcggctac gttgacaccg cctttgcggg
    99241 cttcagcaag cagttggtcg atcagatact gagcacgctc aacaccttct tcacggatga
    99301 ccgattcgat cgcctggagc cagtcgcgag tttcgatcgg atccacgtca tttgggaaac
    99361 gttctgacat gggttattcc ttatctatct aataacgttg agttttctgg aacctgtccc
    99421 attgaactct cgccggaaag ctcaataaga caggttctac gtttagttgc cgcgctttta
    99481 tatgcgcttg atttacaaca tcttctggat aatttttacc agaaaaatca ctaattcttt
    99541 cgttgctcca gacgacgcag agaacgctca cggcggcttt cttcacggct tctgtcgagc
    99601 aaaatttctt cgataaaggc cagatggcga tgcgatgctt cgcgcgcttc ttccggctta
    99661 ccggccataa tcgcttcaaa tatgcgggtg cggtgactac tcaccagcgg cagcatctcg
    99721 cgacgcgaat agagcaattc gaagttctgg cggacattct gggccaacat cggctccata
    99781 caccttagca gatgaagcag aaccacattg tgggccgctt cggtgacggc aatctgatac
    99841 tggagtacgg cgtttgattc cgcgtccaga tcgccagact gctgcgccag ttctatggcg
    99901 tggtggagtt cacggatgcg ttccttgtct tcatcggtac tacgcagcgc ggcgtaataa
    99961 gcggcgatac cttccagggc gtgtcgtgtt tcgagcaagt catactgtga ttcaggatgg
   100021 tcggagagca gctccaccag cggatcgctg aagctttgcc ataggctgct ctggacaaaa
   100081 gtgccgccac cctggcgacg aagcaacaag cccttcgctt cgagacgttg aatcgcctca
   100141 cgcaatgagg gacgggagac gtcaaactgt tttgccagtt cgcgttccgg tgggagtttt
   100201 tcgcccgggc ggagagtgcc ttcgaggatc aaaaactcca gttgctgctc aatcacatcg
   100261 gagagttttg gttggcggat tttgctgtag gccatgagtt cctgtcttaa gccacttgcc
   100321 gaagtcaatt ggtcttacca atttcatgtc tgtgacgcta aagtaacaaa gtattcacct
   100381 tatgtccata caggttttga ttgaaatcat gaaactgtgc acattttaac aacttgacat
   100441 atataacgtt tcaaagttgt aactatgcac aaatgtagac tttacg
//
LOCUS       DAZFDC010000004        86450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFDC010000004 DAZFDC010000000
VERSION     DAZFDC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.004"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            42..1331
                     /gene="uraA"
                     /locus_tag="ACHJ48_000302"
     CDS             42..1331
                     /gene="uraA"
                     /locus_tag="ACHJ48_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO4705937.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1426..2127
                     /gene="hda"
                     /locus_tag="ACHJ48_000303"
     CDS             1426..2127
                     /gene="hda"
                     /locus_tag="ACHJ48_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO4705938.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(2265..2624)
                     /gene="arsC"
                     /locus_tag="ACHJ48_000304"
     CDS             complement(2265..2624)
                     /gene="arsC"
                     /locus_tag="ACHJ48_000304"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO4705939.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2645..4108)
                     /gene="bepA"
                     /locus_tag="ACHJ48_000305"
     CDS             complement(2645..4108)
                     /gene="bepA"
                     /locus_tag="ACHJ48_000305"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO4705940.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRNAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4321..5382
                     /gene="yfgO"
                     /locus_tag="ACHJ48_000306"
     CDS             4321..5382
                     /gene="yfgO"
                     /locus_tag="ACHJ48_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4705941.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5420..6268)
                     /gene="focB"
                     /locus_tag="ACHJ48_000307"
     CDS             complement(5420..6268)
                     /gene="focB"
                     /locus_tag="ACHJ48_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO4705942.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(6290..8302)
                     /gene="hyfR"
                     /locus_tag="ACHJ48_000308"
     CDS             complement(6290..8302)
                     /gene="hyfR"
                     /locus_tag="ACHJ48_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO4705943.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGN
                     HTPGALLLAHTSTTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8332..8745)
                     /gene="hyfJ"
                     /locus_tag="ACHJ48_000309"
     CDS             complement(8332..8745)
                     /gene="hyfJ"
                     /locus_tag="ACHJ48_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO4705944.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVTFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(8738..9496)
                     /gene="hyfI"
                     /locus_tag="ACHJ48_000310"
     CDS             complement(8738..9496)
                     /gene="hyfI"
                     /locus_tag="ACHJ48_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO4705945.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGQEICDRLLRHLSND
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(9493..10038)
                     /gene="hyfH"
                     /locus_tag="ACHJ48_000311"
     CDS             complement(9493..10038)
                     /gene="hyfH"
                     /locus_tag="ACHJ48_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO4705946.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPTNALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(10048..11763)
                     /gene="hyfG"
                     /locus_tag="ACHJ48_000312"
     CDS             complement(10048..11763)
                     /gene="hyfG"
                     /locus_tag="ACHJ48_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO4705947.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(11753..13333)
                     /gene="hyfF"
                     /locus_tag="ACHJ48_000313"
     CDS             complement(11753..13333)
                     /gene="hyfF"
                     /locus_tag="ACHJ48_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO4705948.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(13338..13988)
                     /gene="hyfE"
                     /locus_tag="ACHJ48_000314"
     CDS             complement(13338..13988)
                     /gene="hyfE"
                     /locus_tag="ACHJ48_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO4705949.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(14000..15439)
                     /gene="hyfD"
                     /locus_tag="ACHJ48_000315"
     CDS             complement(14000..15439)
                     /gene="hyfD"
                     /locus_tag="ACHJ48_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO4705950.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVCTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWVLISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(15456..16403)
                     /gene="hyfC"
                     /locus_tag="ACHJ48_000316"
     CDS             complement(15456..16403)
                     /gene="hyfC"
                     /locus_tag="ACHJ48_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO4705951.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGCAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16414..18432)
                     /gene="hyfB"
                     /locus_tag="ACHJ48_000317"
     CDS             complement(16414..18432)
                     /gene="hyfB"
                     /locus_tag="ACHJ48_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO4705952.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHEGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(18432..19049)
                     /gene="hyfA"
                     /locus_tag="ACHJ48_000318"
     CDS             complement(18432..19049)
                     /gene="hyfA"
                     /locus_tag="ACHJ48_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO4705953.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCVQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGREDPLSLTQEQR"
     gene            complement(19302..19772)
                     /gene="bcp"
                     /locus_tag="ACHJ48_000319"
     CDS             complement(19302..19772)
                     /gene="bcp"
                     /locus_tag="ACHJ48_000319"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO4705954.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19772..20344)
                     /gene="gcvR"
                     /locus_tag="ACHJ48_000320"
     CDS             complement(19772..20344)
                     /gene="gcvR"
                     /locus_tag="ACHJ48_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO4705955.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20490..21368
                     /gene="dapA"
                     /locus_tag="ACHJ48_000321"
     CDS             20490..21368
                     /gene="dapA"
                     /locus_tag="ACHJ48_000321"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO4705956.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21385..22419
                     /gene="bamC"
                     /locus_tag="ACHJ48_000322"
     CDS             21385..22419
                     /gene="bamC"
                     /locus_tag="ACHJ48_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO4705957.1"
                     /translation="MAYSVHKSRLATVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            22632..23345
                     /gene="purC"
                     /locus_tag="ACHJ48_000323"
     CDS             22632..23345
                     /gene="purC"
                     /locus_tag="ACHJ48_000323"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO4705958.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23513..24376
                     /gene="ypfJ"
                     /locus_tag="ACHJ48_000324"
     CDS             23513..24376
                     /gene="ypfJ"
                     /locus_tag="ACHJ48_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO4705959.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            24391..26406
                     /gene="tmcA"
                     /locus_tag="ACHJ48_000325"
     CDS             24391..26406
                     /gene="tmcA"
                     /locus_tag="ACHJ48_000325"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO4705960.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAIFWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIITAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            26480..27178
                     /gene="ypfH"
                     /locus_tag="ACHJ48_000326"
     CDS             26480..27178
                     /gene="ypfH"
                     /locus_tag="ACHJ48_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4705961.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(27259..27459)
                     /gene="ypfN"
                     /locus_tag="ACHJ48_000327"
     CDS             complement(27259..27459)
                     /gene="ypfN"
                     /locus_tag="ACHJ48_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO4705962.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(27487..28614)
                     /gene="dapE"
                     /locus_tag="ACHJ48_000328"
     CDS             complement(27487..28614)
                     /gene="dapE"
                     /locus_tag="ACHJ48_000328"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO4705963.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKAVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(28618..28974)
                     /gene="yffB"
                     /locus_tag="ACHJ48_000329"
     CDS             complement(28618..28974)
                     /gene="yffB"
                     /locus_tag="ACHJ48_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO4705964.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNIITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQKFFHEV"
     gene            29355..29414
                     /gene="ypfM"
                     /locus_tag="ACHJ48_000330"
     CDS             29355..29414
                     /gene="ypfM"
                     /locus_tag="ACHJ48_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO4705965.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(29513..32626)
                     /gene="acrD"
                     /locus_tag="ACHJ48_000331"
     CDS             complement(29513..32626)
                     /gene="acrD"
                     /locus_tag="ACHJ48_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO4705966.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATAEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(32790..34490)
                     /gene="narQ"
                     /locus_tag="ACHJ48_000332"
     CDS             complement(32790..34490)
                     /gene="narQ"
                     /locus_tag="ACHJ48_000332"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO4705967.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGTPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLGRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            34696..36675
                     /gene="aegA"
                     /locus_tag="ACHJ48_000333"
     CDS             34696..36675
                     /gene="aegA"
                     /locus_tag="ACHJ48_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO4705968.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLIRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            36743..37318
                     /gene="nudK"
                     /locus_tag="ACHJ48_000334"
     CDS             36743..37318
                     /gene="nudK"
                     /locus_tag="ACHJ48_000334"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO4705969.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLID"
     gene            37444..38487
                     /gene="ypfG"
                     /locus_tag="ACHJ48_000335"
     CDS             37444..38487
                     /gene="ypfG"
                     /locus_tag="ACHJ48_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO4705970.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSDLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38582..40585)
                     /gene="tkt"
                     /locus_tag="ACHJ48_000336"
     CDS             complement(38582..40585)
                     /gene="tkt"
                     /locus_tag="ACHJ48_000336"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4705971.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(40605..41555)
                     /gene="tal"
                     /locus_tag="ACHJ48_000337"
     CDS             complement(40605..41555)
                     /gene="tal"
                     /locus_tag="ACHJ48_000337"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4705972.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            41844..44123
                     /gene="maeB"
                     /locus_tag="ACHJ48_000338"
     CDS             41844..44123
                     /gene="maeB"
                     /locus_tag="ACHJ48_000338"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO4705973.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            44416..44751
                     /gene="eutS"
                     /locus_tag="ACHJ48_000339"
     CDS             44416..44751
                     /gene="eutS"
                     /locus_tag="ACHJ48_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO4705974.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            44764..45243
                     /gene="eutP"
                     /locus_tag="ACHJ48_000340"
     CDS             44764..45243
                     /gene="eutP"
                     /locus_tag="ACHJ48_000340"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO4705975.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            45218..45919
                     /gene="eutQ"
                     /locus_tag="ACHJ48_000341"
     CDS             45218..45919
                     /gene="eutQ"
                     /locus_tag="ACHJ48_000341"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO4705976.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            45916..46647
                     /gene="eutT"
                     /locus_tag="ACHJ48_000342"
     CDS             45916..46647
                     /gene="eutT"
                     /locus_tag="ACHJ48_000342"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO4705977.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPLGCQAIVGLSDEDLHRLSHQPLRYLGH
                     DHLVPEASHGRDAALLNLLRTKVREAETVAAQVFITRSFEVLRPDILQALNRLSSTVY
                     VMMILSVTKQPLTVKQIQQRLGETQ"
     gene            46644..47660
                     /gene="pta"
                     /locus_tag="ACHJ48_000343"
     CDS             46644..47660
                     /gene="pta"
                     /locus_tag="ACHJ48_000343"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4705978.1"
                     /translation="MIIERCCELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            47699..47992
                     /gene="eutM"
                     /locus_tag="ACHJ48_000344"
     CDS             47699..47992
                     /gene="eutM"
                     /locus_tag="ACHJ48_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO4705979.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            48099..48386
                     /gene="eutN"
                     /locus_tag="ACHJ48_000345"
     CDS             48099..48386
                     /gene="eutN"
                     /locus_tag="ACHJ48_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO4705980.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPHGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            48398..49801
                     /gene="eutE"
                     /locus_tag="ACHJ48_000346"
     CDS             48398..49801
                     /gene="eutE"
                     /locus_tag="ACHJ48_000346"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO4705981.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            49812..50648
                     /gene="eutJ"
                     /locus_tag="ACHJ48_000347"
     CDS             49812..50648
                     /gene="eutJ"
                     /locus_tag="ACHJ48_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO4705982.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTFAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            50638..51825
                     /gene="eutG"
                     /locus_tag="ACHJ48_000348"
     CDS             50638..51825
                     /gene="eutG"
                     /locus_tag="ACHJ48_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO4705983.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQLVGLYA
                     AAQ"
     gene            51925..53151
                     /gene="eutH"
                     /locus_tag="ACHJ48_000349"
     CDS             51925..53151
                     /gene="eutH"
                     /locus_tag="ACHJ48_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO4705984.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            53148..54551
                     /gene="eutA"
                     /locus_tag="ACHJ48_000350"
     CDS             53148..54551
                     /gene="eutA"
                     /locus_tag="ACHJ48_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO4705985.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            54563..55924
                     /gene="eutB"
                     /locus_tag="ACHJ48_000351"
     CDS             54563..55924
                     /gene="eutB"
                     /locus_tag="ACHJ48_000351"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO4705986.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            55945..56832
                     /gene="eutC"
                     /locus_tag="ACHJ48_000352"
     CDS             55945..56832
                     /gene="eutC"
                     /locus_tag="ACHJ48_000352"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO4705987.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKVWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTLALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            56842..57501
                     /gene="eutL"
                     /locus_tag="ACHJ48_000353"
     CDS             56842..57501
                     /gene="eutL"
                     /locus_tag="ACHJ48_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO4705988.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            57514..58014
                     /gene="eutK"
                     /locus_tag="ACHJ48_000354"
     CDS             57514..58014
                     /gene="eutK"
                     /locus_tag="ACHJ48_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO4705989.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGVTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            58060..59112
                     /gene="eutR"
                     /locus_tag="ACHJ48_000355"
     CDS             58060..59112
                     /gene="eutR"
                     /locus_tag="ACHJ48_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO4705990.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(59118..60017)
                     /gene="hemF"
                     /locus_tag="ACHJ48_000356"
     CDS             complement(59118..60017)
                     /gene="hemF"
                     /locus_tag="ACHJ48_000356"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO4705991.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGKDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(60021..60890)
                     /gene="amiA"
                     /locus_tag="ACHJ48_000357"
     CDS             complement(60021..60890)
                     /gene="amiA"
                     /locus_tag="ACHJ48_000357"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO4705992.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            61104..61529
                     /gene="ypeA"
                     /locus_tag="ACHJ48_000358"
     CDS             61104..61529
                     /gene="ypeA"
                     /locus_tag="ACHJ48_000358"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO4705993.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            61516..61965
                     /gene="yfeZ"
                     /locus_tag="ACHJ48_000359"
     CDS             61516..61965
                     /gene="yfeZ"
                     /locus_tag="ACHJ48_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO4705994.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            62026..62601
                     /gene="yfeY"
                     /locus_tag="ACHJ48_000360"
     CDS             62026..62601
                     /gene="yfeY"
                     /locus_tag="ACHJ48_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO4705995.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADAVDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            62697..63596
                     /gene="yfeX"
                     /locus_tag="ACHJ48_000361"
     CDS             62697..63596
                     /gene="yfeX"
                     /locus_tag="ACHJ48_000361"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO4705996.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNIWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(63654..64958)
                     /gene="pbp4b"
                     /locus_tag="ACHJ48_000362"
     CDS             complement(63654..64958)
                     /gene="pbp4b"
                     /locus_tag="ACHJ48_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO4705997.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYISGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     THWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(64963..66387)
                     /gene="murP"
                     /locus_tag="ACHJ48_000363"
     CDS             complement(64963..66387)
                     /gene="murP"
                     /locus_tag="ACHJ48_000363"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO4705998.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66391..67287)
                     /gene="murQ"
                     /locus_tag="ACHJ48_000364"
     CDS             complement(66391..67287)
                     /gene="murQ"
                     /locus_tag="ACHJ48_000364"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO4705999.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            67451..68308
                     /gene="murR"
                     /locus_tag="ACHJ48_000365"
     CDS             67451..68308
                     /gene="murR"
                     /locus_tag="ACHJ48_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO4706000.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATAQHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGCDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            68437..69228
                     /gene="ucpA"
                     /locus_tag="ACHJ48_000366"
     CDS             68437..69228
                     /gene="ucpA"
                     /locus_tag="ACHJ48_000366"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO4706001.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            69386..70402
                     /gene="cysP"
                     /locus_tag="ACHJ48_000367"
     CDS             69386..70402
                     /gene="cysP"
                     /locus_tag="ACHJ48_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO4706002.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            70402..71235
                     /gene="cysT"
                     /locus_tag="ACHJ48_000368"
     CDS             70402..71235
                     /gene="cysT"
                     /locus_tag="ACHJ48_000368"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO4706003.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            71235..72110
                     /gene="cysW"
                     /locus_tag="ACHJ48_000369"
     CDS             71235..72110
                     /gene="cysW"
                     /locus_tag="ACHJ48_000369"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO4706004.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            72100..73197
                     /gene="cysA"
                     /locus_tag="ACHJ48_000370"
     CDS             72100..73197
                     /gene="cysA"
                     /locus_tag="ACHJ48_000370"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO4706005.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIEPRDEELALAQSA"
     gene            73331..74242
                     /gene="cysM"
                     /locus_tag="ACHJ48_000371"
     CDS             73331..74242
                     /gene="cysM"
                     /locus_tag="ACHJ48_000371"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO4706006.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMADRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITYFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(74245..74612)
                     /gene="yfeK"
                     /locus_tag="ACHJ48_000372"
                     /pseudo
     CDS             complement(74245..74612)
                     /gene="yfeK"
                     /locus_tag="ACHJ48_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
     gene            74717..75568
                     /gene="pdxK"
                     /locus_tag="ACHJ48_000373"
                     /gene_synonym="thiJ"
     CDS             74717..75568
                     /gene="pdxK"
                     /locus_tag="ACHJ48_000373"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO4706007.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMLVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(75611..76120)
                     /gene="crr"
                     /locus_tag="ACHJ48_000374"
     CDS             complement(75611..76120)
                     /gene="crr"
                     /locus_tag="ACHJ48_000374"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO4706008.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(76161..77888)
                     /gene="ptsI"
                     /locus_tag="ACHJ48_000375"
     CDS             complement(76161..77888)
                     /gene="ptsI"
                     /locus_tag="ACHJ48_000375"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO4706009.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(77933..78190)
                     /gene="ptsH"
                     /locus_tag="ACHJ48_000376"
     CDS             complement(77933..78190)
                     /gene="ptsH"
                     /locus_tag="ACHJ48_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO4706010.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(78574..79545)
                     /gene="cysK"
                     /locus_tag="ACHJ48_000377"
     CDS             complement(78574..79545)
                     /gene="cysK"
                     /locus_tag="ACHJ48_000377"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO4706011.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFINKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(79730..80491)
                     /gene="cysZ"
                     /locus_tag="ACHJ48_000378"
     CDS             complement(79730..80491)
                     /gene="cysZ"
                     /locus_tag="ACHJ48_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO4706012.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            80721..81719
                     /gene="zipA"
                     /locus_tag="ACHJ48_000379"
     CDS             80721..81719
                     /gene="zipA"
                     /locus_tag="ACHJ48_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO4706013.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            81790..83805
                     /gene="ligA"
                     /locus_tag="ACHJ48_000380"
     CDS             81790..83805
                     /gene="ligA"
                     /locus_tag="ACHJ48_000380"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO4706014.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTYCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            83807..84025
                     /gene="ypeB"
                     /locus_tag="ACHJ48_000381"
     CDS             83807..84025
                     /gene="ypeB"
                     /locus_tag="ACHJ48_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO4706015.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(84022..85020)
                     /gene="yfeH"
                     /locus_tag="ACHJ48_000382"
     CDS             complement(84022..85020)
                     /gene="yfeH"
                     /locus_tag="ACHJ48_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO4706016.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            85110..86036
                     /gene="yfeR"
                     /locus_tag="ACHJ48_000383"
     CDS             85110..86036
                     /gene="yfeR"
                     /locus_tag="ACHJ48_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4706017.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNTLMAGREGDPLYQI"
     gene            complement(86027..86368)
                     /locus_tag="ACHJ48_000384"
     CDS             complement(86027..86368)
                     /locus_tag="ACHJ48_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO4706018.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQHI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIGIYI"
BASE COUNT        20520 a        22354 c        23655 g        19921 t
ORIGIN      
        1 tgagtaaagc gcctataaca cataatacag aggataatac tatgacgcgc cgtgctatcg
       61 gggtgagtga aagaccgcca cttttacaga caatcccgct tagtttgcaa catttgttcg
      121 ccatgtttgg tgcaaccgtc ctggtgcccg tcttatttca tattaacccg gcgactgtac
      181 tgttatttaa cggtattgga acgctgctgt atctcttcat ctgtaaaggg aaaattccgg
      241 cttatcttgg ttccagcttt gcctttattt caccggtatt gttactgttg ccgttagggt
      301 atgaagtcgc gctgggcggc tttattatgt gcggcgtgct gttctgcctg gtttctttta
      361 tcgtgaagaa agcggggacc ggctggctgg acgtgctgtt tccacctgcg gcaatgggcg
      421 caatcgttgc cgtcatcggt ctggagctgg cgggcgtagc tgccggtatg gcgggtttac
      481 tcccggctga agggcaaacg ccagactcca aaaccatcat catctctatt accaccctgg
      541 cggtcacggt tttaggttcc gtgctgttcc gtggtttcct ggcaattatc ccgattttaa
      601 ttggcgtgct ggtggggtac gcgctctctt tcgcaatggg aattgtcgat accacgccga
      661 ttattaatgc tcactggttt gcgctgccaa ccctctatac gccgcgcttc gagtggtttg
      721 ccattctgac tattctgcca gcggcgttag tggttattgc cgaacacgta gggcacctgg
      781 tagtaacggc taatatcgtc aaaaaagatc tgctgcgcga tccaggtctg caccgttcga
      841 tgtttgctaa cggcttgtcg accgtgatct ccggcttctt tggctccacg ccaaacacca
      901 cctacgggga aaacattggc gtgatggcga tcacccgtgt ttacagtacc tgggttatcg
      961 gcggggcggc gattttcgct atcctgcttt cctgcgtcgg taaactggct gccgctatcc
     1021 agatgatccc attgccggtg atgggcggcg tttcgctgct gctttatggt gtcatcggtg
     1081 cttccggtat tcgtgttttg atcgaatcga aagtggatta caacaaagca cagaacctga
     1141 tcctgacttc cgtgattttg atcatcggcg tcagtggcgc gaaggtaaac attggcgcgg
     1201 cggagctgaa aggtatggcg ctggcgacca tcgtcggtat cggcttaagt ttgattttca
     1261 aactgattag tgtgctgcgc cctgaagaag tggtgctgga cgcagaagac gcggatataa
     1321 cagacaaata atgtaccgac cgggcagtgt tgctgcccgg ttcaaacatc atgggattct
     1381 gtggtaaact tctcgcgatt ttgtgaaatc ctggttgagg tatctctgaa cacaccggca
     1441 cagctctctt tgccacttta tcttcctgac gacgaaacct ttgcaagttt ctggccgggg
     1501 gataactcct ctttactggc cgcgctgcaa aacgtgctgc gtcaggaaca tagcggttac
     1561 atctatctct gggcacgcga aggcgcgggg cgcagccatc tgctgcacgc ggcttgcgcg
     1621 gaattgtcgc agcgtggcga tgcggtgggc tatgtcccgc tggataaacg cacctggttt
     1681 gttccggaag tgctcgacgg tatggagcat ttgtcgctgg tctgtatcga caacattgag
     1741 tgtattgcag gcgatgagtt gtgggagatg gcgattttcg atctctacaa tcgaattctg
     1801 gaatcgggca aaacacggtt gttgatcacc ggcgatcgtc caccgcggca gttgaatctg
     1861 ggattaccgg atctcgcgtc gcgactcgac tgggggcaga tctacaaatt gcagccactt
     1921 tctgatgaag ataagttgca ggcgctacag ttacgcgcgc gtttgcgtgg ttttgaactg
     1981 ccggaagatg tggggcgttt cttgctgaag cggctcgaca gagaaatgcg cacgctattt
     2041 atgacgttgg atcagttgga tcgtgcgtcg attaccgcgc aacgtaagct gaccattccg
     2101 tttgtgaaag aaattctgaa gttgtagata aggtgtttat tgtcggatgc ggcgcgaacg
     2161 ccttatccga actacgcgaa gttaatccat agaattcgta ggccggataa gacgtggcaa
     2221 gcgtcgcatc cggcaatgtg cttcaactag cgatggagca catcttaacc cacgatttcc
     2281 agtacctgct ccggtggacg accaatccgc gctttgccat tcgcgaccac aatcgggcgc
     2341 tccatcagct tcggattatc caccattgcc tgaatcagcg cctcttcgct aagcgaactg
     2401 tcagccaggt tcagttcttt ataaagatcc tctttctggc gcatcaattc acgggcgcta
     2461 ttcatcccca gcattttcag caaatcgcgc agcgttgccg catcggcggg tgtctcaagg
     2521 tagagcacca cttctggctc cacgccgttt tctttcagca ggttcagcgt ttcccggctc
     2581 tttgaacagc gtgggttatg gtagattttt acctgtttgg tcattgttct tcctttaatg
     2641 cgaattacat cttggtataa ggcttaaagc gttcctgcag ctggcgcaac tggtcgatgc
     2701 gcgcatcgta acgcgcttgt tgcaggctgc ctaatttcac ctgcgaactg gcgctactca
     2761 acagcgaaat ggcctgatcg agtcgtccgg cgagcgcata accttctgct cgcgcagcca
     2821 gctcctgatc gcggttattt agcgcggctt ccgcctgtgc cagcaaatcc cagccgttgc
     2881 tgtcatcttt attattaaag gtgtagcgat taagaatatt ggccgcttct tgtggttgac
     2941 cgccttgtag ataagcgttc gccaggttga gctgcaacac aggattggtg cgcaaatcgc
     3001 gggcattttt cagacgattg atcgcctcat tggctttgtt ttgcccaaga tcgatatcag
     3061 tagccagatc gagataccat gcgttgccag gttctgccgc cagtaacggt tgcagcgttt
     3121 ttcgcgcctc gtcgtattta ttggcttcca tcgcctgtaa agcacgacca tattgcgccg
     3181 ctcgttgctg acgaacgttt cctttcgccc attcatccag caaatcactg gtgagctggt
     3241 tacgtccgga attatacatc cccagtgtgc gcgctttcgc cagatagaaa tcttccgacg
     3301 actgcaccac catcgggcgc atctgattag cacggttgcg ggcatctgcc agacgacttt
     3361 ccggcaacgg gtgagtcagt aaaatttccg gcgggcgcga ggagtaacgc gcctgatcga
     3421 gtaatttttc gaggaaggtt ggcatcgcct gcggatcgaa tcccgagcgt tgcagcacct
     3481 gaataccaat gcggtccgct tcctgttcat tttgctgggt gaaactgatc atcccctgac
     3541 gcgttcccgc cagtgtaccg gtcagcgccg ccatccccgc ctgcggactg gccatcgcca
     3601 gtaaaataga acctaacgcg ccgacccagg tcagcggcgc gttgcgctgc tgatcttcca
     3661 tcgctcgcgc caggtgacgt tgggtgacgt gggagatttc gtgcgccata actgaagcca
     3721 gttgactttc gttatcggaa taacggaaca gggcagagtg cagcaccacg ttgccgccaa
     3781 agaaagcaaa ggcgttaatt tcgtcgttgt tgatcagaaa gaaatgaaac ggtgtcttaa
     3841 ccgaattggc atgcgaaacc agacgcatcc ccagcgaatt aatatattgc gttaacagcg
     3901 ggtcattaat taacggcgcg ctgccgcgta gctggcggac ataatagtcg cccatctgca
     3961 tttcctgacc aatggaaagc gtgcttcctg cggaggttcc catatccggc aaggtgtctg
     4021 cgctgtcggc aaatgccggg gctacctgac caatggtcat agcagcaatg agggttgcaa
     4081 ccaggttttt tttcaactgc ctgaacataa cctctatcct gtatttctga agatagtcat
     4141 ttgaccgata ctgtgaaata tcgttcccac cgcgccgcct ttgagtgtaa cggcgaagat
     4201 tagcccactc aaatgacttt tcagtttcct gaatccgtca taaaaagcga agtctttttt
     4261 gtgacatttc gatacaattg cggatcgcaa tcccgctctt gaggttcagg gaaggtcttt
     4321 atgctcgaaa tgttgatgca atggtatcgc cgccgtttta gcgacccgga agcgattgcc
     4381 ttgctggtta ttttagttgc cggatttggc attatctttt tctttagtgg cctgcttgct
     4441 ccgttgctgg tggctattgt gctggcctat ttgctggaat ggccaaccgt gcgcctgcaa
     4501 tctattggct gctcccgccg ctgggcgacg tcgattgtat tggtggtttt cgtcggtata
     4561 ttgctactga tggcgttcgt ggtactgcct atcgcctggc agcagggcat ctacttaatc
     4621 cgcgatatgc cggggatgct caataagctt tctgactttg ccgccacgtt gccgcgccgc
     4681 tatccggcgt taatggatgc gggcattatt gatgcaatgg ccgaaaatat gcgcagtcgg
     4741 atgctgacca tgggcgattc ggtggtgaaa atttccctcg cctcgctggt cggtttgctg
     4801 accattgccg tctatctggt gctggtacca ttgatggtct tcttcctgct gaaagacaaa
     4861 gagcagatgc tgaatgccgt tcgccgggtg ctgccgcgta accgtggact ggcaggacag
     4921 gtgtggaagg agatgaatca acaaatcacc aactatatcc gcggcaaagt gctggagatg
     4981 atcgtagtag ggatcgccac ctggctggga ttcttactct tcgggctgaa ctattcgctg
     5041 ctgctggcgg tgctggtcgg cttctcggta cttattccgt acattggcgc atttgtggtg
     5101 accattccgg tggttggcgt ggcgctattc cagtttggtg caggcactga attctggagc
     5161 tgtttcgcgg tgtatctgat cattcaggcg cttgacggca acctgttagt accggtgttg
     5221 ttctccgaag cggttaacct gcatccgctg gtgattattt tatcggtggt gatcttcggt
     5281 ggtttgtggg gattctgggg cgtattcttc gccattccat tggcgacgct gatcaaagcc
     5341 gtgatccacg cctggcccga tgggcaaata gcgcaagaat aatcttcagc ccgacgaact
     5401 gtttcgccgg gctgattttt caggattcct gacgtaaata aatagcccga taacaaatac
     5461 tcaccagcac tgcaccgccg ataatattac ccagcatcac cgggagcaga ttggcggtaa
     5521 taaaatggct gaccgtcagt gcaggaaaat tgtctgcgct actgtgcgcc agttgccaga
     5581 aggaggtagg ggcgaaatgg cgaatggcaa tggcgaacgg aatcacaaac aaattggcga
     5641 tacagtgctc aaagccactg gcgacaaaca gggtgatggg caaaatcatg gcgacgattt
     5701 tgtcgcataa cgaacgcccg caataactca tccacagcgc caggcaaacc atcagattgc
     5761 acataatgcc gaggctgaca gattcagtaa atgtatgatg cattttgccc tcggcgcagt
     5821 gtaaaaccgc gactccccac atcgcgtttt cactcatcac cagcccggaa aaccagatta
     5881 acaaactgaa acaggcaata cctgccagat taccgcaggc caccagaagt gcgttaatca
     5941 gccaagttcg ccaactaata acgccccgac tttttgccat caccgtcatt accgacgagg
     6001 tgaacaggct ggtgccgcaa accgccagca aaataaagcc gagtgtaaag caaaggccgc
     6061 ccaccagatg ggttaatgcc tgtgaagacg gggcatcggc gataacggaa aggtaaaaag
     6121 taaatccgat cgccataaac acgccagcgg acatcgccat taaaaagacc gacactttac
     6181 tgcgggcaat tttatgggcg gcaatccgtt cagcgattgc cgcttttctg gcgctcaact
     6241 gcaagtcgaa agagagtttg tttctcatgc atactcctgc aaaagcagat tacaacacct
     6301 cgcgaaccga gatccccaga cgctgcattc gtgacagcag cgtggtgcgc ttcatcccta
     6361 atcgtgtcgc cgcgccacgg ggcccggcaa caatgccatt ggtttctcgc aatacctgaa
     6421 caatgcgctg acgttcttct tcgtcattct ctggcgtcgt cgggtgcagc aactgcgcca
     6481 ttgaactgcg aagcgctgaa tcttcattta gcgtcggcag taaacggctt tgtcggacat
     6541 taagatgtaa attcagactg ttaccgcgag tcagcagtac cgctcgctca atcacgtttt
     6601 ccagctcgcg cacgttgccc ggccaatccc acgacatcaa ctggcgtagt gcctcggtcg
     6661 ggatggcgtc aattgcgcga ttcatatggc gcgccatttt ttgcgtgaaa tgttttgcta
     6721 aaagagggat atcttccgga cggtctcgca gcggcggcaa ttccagtggg aagacattca
     6781 ggcgataaaa gagatcgctg cgaaactggc gatcttcaac catttgccac aaatcacggt
     6841 tggtggcggc aatgactctg acattcaccg ggatcgttct actcccgccg agacgctcaa
     6901 tttcccgctc ctgcaatacg cgcagcaatt taggctgaag ttctaacggc agatcgccaa
     6961 tttcatcgag aaacaacgta ccgccatcgg caatttcaaa acgaccacga tgggtattaa
     7021 tcgcaccagt aaaggcccct ttgtcatgac cgaataactc actttccaga agactggcgg
     7081 ggatggcagc gcagttgatt ttgaccagcg gcttgtcgcg tcgcgggcta agttgatgga
     7141 tcgctctggc gatcacctct ttgccggttc cggtttcacc acaaatcaac accgtactgt
     7201 cgctcttcgc cacaatatct acctgctgga gcaggtcttc cattgcctgg ctttgataga
     7261 taatgtcacc gacgcccaga ttcgaaagga gctgctcgct aagctggtgg ttttcttgct
     7321 gcaaactttc ctgcaaatcg gtcatgctac gccaggcatc ggcattgcca acggcaatag
     7381 cgatgcgatc ggctatgtgt tgtagtagct ggcagttttc ctcactaaag agagtggtag
     7441 aggtatgcgc cagcaacaat gcacccggtg tatggttgcc aaaggtaagc ggtatcagga
     7501 gcgaagattc gcagccgtta gatgcaagta agtgtaataa cgggtcgcgt tgccagagaa
     7561 acagatcgtc tgcacggtgc gtcaaggtcg gttgtcgggt ttgtagcgtt tgtgtcagca
     7621 atacactgtc gccaggcata ttgcgtggca gacacgcaca atgtgaggca gaaagatcgc
     7681 tgcaccacag gctgaacttc tcgttctttc gatgatcgcc cagtaccata ctgactgaag
     7741 ccagaccgaa aaaatgatgg atctcacgag cgacgtcagc gatcagatcg tcgagatcaa
     7801 gatgtgacag cactgcatta gtgatatcca ccagtacccg atgatgatcg cgatctttct
     7861 ccagcaaacg cagcgccacc tgttgttctt catcattgag ccatgattgc agaaccgggg
     7921 tgataatgcg tgccagccag ttaaacagtg tgcaatcctg gggagtaaaa cgactgccgt
     7981 gggtgcgaat aaagcgtagc tccgccattc cactactgcc gagaaggacg gcgtgacgcg
     8041 aataatcgac catctcgccc tgatactctg ttgttgccgt cacatcgctg ccatcatcat
     8101 ggtagcggta atagaagttg cttttgagaa catgcagttc aataagttgt accgggctga
     8161 acggcggcgg cagcgggatg aaggcgcgta ataaagacgc cttgccgtgt ttctgcgcta
     8221 accgctctgc gagcattccg tttaccgctt caattgttat tccttgcggc ggggcaaaca
     8281 tcgcctcgtc tgacatagcc atcaacagtc cttattaatg agaaatcctt ctcacgcaat
     8341 ttttctggcc atcagataga tagccggttc cagcacaatt tcatccaaca gatgaatgaa
     8401 ttgaatgcta cgcgcttgcc acacagacgt cgcgtcatct gccagtggcg cgaatgcccg
     8461 ggtcaacagg gcggtgtggc tggcgtcaat aacaatctca ccaaaagtca tcacccccag
     8521 catcttacgt cgggcttgct cctcttcgac cagtgcaagc caatcttcgt attccgtcag
     8581 tgggcagcgg aaggtgacat tcagacaatc aatcacgcca acgtgatgac cgatagccag
     8641 cgagtaatac attacctggg agctgtgttc cggcatctcg tcgctactgg cgacaaactt
     8701 ttttcgcagc gtccagaaaa caatttcccc gcactcttca gtcatgtaac ccccggagta
     8761 cgcgaaagag ttgctgaacg atactattga gacgtggatc gtcggcatcg cgcaaccagg
     8821 tgttaacccg atttcctgta ggatcgttgc ttaaatggcg caggagccga tcgcaaattt
     8881 cttgcccctg acgatagccc gccagccgcc gcgcttctcg ctcaatggcg atacgctgtg
     8941 atggcgggat ctgcggccac aacggttgca tagtcacccc ggtgggatcg cgataatcca
     9001 ccgcgtgaat cttctgttgc agcaaaccga gcgccacggc gaaaccgtga atggtggcgg
     9061 ccggtgttgg cgggcagccg gggatccaaa catcaatggg gacaatggtg tcgctgccgc
     9121 cccagacgct gtagagatcg tggaaaatac cgccgccgac accgcacgcg ccgtaggaaa
     9181 cacagatttt atgatcgggg gcagactcat acgcccgaag tgcaggcata cgcatcgccc
     9241 gggtgactgc gccagtaaat aacaaaatat cggcgtgacg cggtgatgaa acaaccttaa
     9301 tgccaaaacg ttctgcgtcg aatacgggtg tgatggcggc aaaaatttcg atttcacagg
     9361 cgttgcagcc gccgcaatcg acgcgataaa cgtaggctga gcgacggata tcctgtagca
     9421 aatgccgctt catcttttgc gtttgctcgt ccagcgtgat gggctggctg acatgttgtg
     9481 taagcactgg actcatagct gctccttagc caccagcggt acatctgtat cgtcgttgat
     9541 cagcgtcgcg cgttgtttgc actccgggca gacgctcgct tgcgcccgca acatttcgcg
     9601 gttttgtggc gcattttgtt gctgtgccaa caattcagca gccagtgcga cggttttttg
     9661 cggggcaaac gggcgttcgc aacggctgca acgttgtaga tggaacgtcg cgcgggtata
     9721 gagatcggct ttattggtga cggtcagttc aaagttattg gtaagctgga tggctctggt
     9781 cgggcacact tcttcacaac gtccgcagta aatacaacgc cccagataga gctgccaggt
     9841 gcgcgaattt tgctggtcgt cggtctggat agtcagcgca tttgtcggac aagcacaggc
     9901 gcaggcacca caggcaatac attggctggg catcaggtcc ggtttcccgc gaaagcctgg
     9961 gctgacctcc agtggcgcga agggatattt caccgtcgcg gttccggcgc gcataatagt
    10021 tttcagtaac ttcagcatct tctgtcctta cttcagcggc gaacggttac gatcaatgcc
    10081 gtagcgttcg atctctttat acggcacggt ttttgactgg cgcttgcgca catcaaccag
    10141 cgtcacacgg tcggtacagg agtagcaggg atcaaggcta ccgataatca gcggtgcgtc
    10201 agaaacggta ttgccgcgca gcatgtaacg caacaccggc cagttggcgt aggtggcggc
    10261 acggcagcgc cagcggaaca atttttggtt atcaccgagc atgctccagt gcacgtcttc
    10321 accgcgtggc gcttcaacaa agccgagcgc gaatgcgtga ggtttatagc taaagccttc
    10381 ggtcagcagt ggggtatccg gcatgttgtc gagagcaaat tccagcattg ccagcgaatc
    10441 aaacgtctct ttgacacgga ccatcacgcg ggagaaaaca tcgccgccgg tgaaggtaaa
    10501 cagtgttttt ggaatattgc cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc
    10561 aaaaccactg ccgcggatca gcggccctac agggctataa tcacgggcga tttgtcggtc
    10621 gagaatgcca atgccctgag tgcgttgttc catattcggc gtggcaagca gcatttctac
    10681 cagctccgac acgtcggcgc gcatctcgcg caccagtttc agcgtttgca gacgttgctc
    10741 tttgagaata tcgcggcgaa caccaccaat cagattcaga ccgtaggttt tacgcgaccc
    10801 ggtcagcaat tccgccattg tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc
    10861 ggtatcaaat ccaacaaaat ggcaggagag gccaaggttg agcaaatgac tgtgtagccg
    10921 ttcgacttcc agcagaatcg agcgaatggt atgcgctcgt tgcggcacct caatccccag
    10981 tgcattttca accgagttgg tataggccac actgtgggca aaaccgcaaa tcccacacac
    11041 gcggtccgat aagaaggtca cttcgttgta gcccatccgc gtttctgcca gtttctccat
    11101 gccgcgatgg acataaaaca gacggtaatc agcatcgaca atttgctcgc catccacaaa
    11161 caagcggaag tgacccggtt catcggaggt gatatgcagc gggccgacag ggatcacccg
    11221 cgcaccgctg ttgccctcat tgataaacgg atacgtttcg gaatcagtcg tcggttcagg
    11281 gcgcagtcga taatccatcg catctttgcg cagcggatgc atatcttccg gccagtcatc
    11341 gggcaacacc aggcgacgct gatccggcag gccaaccgga atcagcccgt acatatcacg
    11401 aatttctcgc tcgccccaga ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg
    11461 actgtcggca tcgaccagcg ccttcaccac aatccagcat ttttcggccc cttccattga
    11521 aagggcataa taaaccgcgt aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac
    11581 cggaagccag ccatcatgtt gataataaag atactgtaca acgtcaggca gcagattgat
    11641 tttcaccgta atggtgacct gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg
    11701 gaactgcgtt ttcagggcgg cgagaatcgc ttcgccacga tttgacgatg aattaacgtt
    11761 cactgtgttt ctccgaaaca gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac
    11821 gttgtgcagg caggtcgtgc gtccctgaga ggactatagt ggaagcgccc gccaggatcc
    11881 tgatgacagg ttgtggaata tgcgttccca tcgccagcat catgaccagc agaatcacca
    11941 ttggcgaggt ggtcaaccag ccgagatcac cccggttaac ggcctgcggc ggtttcgcca
    12001 ttaacacccg cgcagccatc cgtaccaggc ccgccagcac cagcgttaac agcaataaca
    12061 gcaggacgat aatcagcagg tgattacgtg ccagtccggc ggtaacggtc ataaattcgc
    12121 taagaaaaat gttgaagggc ggcatccctg ccagcgccag cgcaccgcct ccaaacagca
    12181 cggcggtaaa tggcatgatt ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt
    12241 acttgagcag tacattgccg gaaccgcaga acagcagcgt ttttgccaga ctgtggttta
    12301 aggtgtgcag cagcgcggca aaaattccca gcggcccgcc aatgcctagc gccaccgcga
    12361 ccagtcccat gttctccacg ctggagtacg ccagcagacg cttaatgtcc cgctgtacca
    12421 gaatgaaaaa tgccgccacg gcaaccgaca acatgccgaa gatgagcaac aaccggttgg
    12481 ggaaatcgct gccgatggcc tggcaaataa tgatgtaata gcgaatcagc accaacagcg
    12541 cgcagttcag caatacggcg gagagtaggg cgctgaccgg actcggcgct tcactgtgag
    12601 catccggcag ccaggcgtgc atgggaaata gcccggtttt ggtaccaaag ccaattagca
    12661 caaacacaaa ggccaacagc attaatgttg gatcaagcaa ggacgattgc ttaagaacct
    12721 cgctccagaa tatcgccatt tctgcctgcg gcataacgct ggcggcgttg gcgtatacca
    12781 gcacggtacc gaacagacca aaagcgacac caacagtaca aataatgatg tacttccatg
    12841 ctgcttccag cgatgaacgc tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg
    12901 tggcttcgat cgccgcccac atcacaatca ggttattgct ggtaacaacc agcagcatgg
    12961 tgaacaaaaa cagatggaag aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc
    13021 cgtgctcaac ttcatgacgc atgtagccaa tcgagtaaac accggtgaga aagccaatca
    13081 caccaagaat ggcgaggaac aaaccgccca gaccatcaat atgaagccac agtcccgcag
    13141 cgaatatttc tcctgcatca gcggcagttt ggaccaccca gagtgccaga atcagcagca
    13201 gtgtgatccc taagctatgt aatactgtca ccgtgcgagt cgcagagtgc cctcgtttcc
    13261 ggcaggcaaa acagagcagc gaaaaaagca gcggagtgag caggagtaaa gcgaacatca
    13321 cagaataact catctcatta tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg
    13381 tgggtacgcc atatttttct tgccagtaac accatcacaa tgacggcgaa gatggcgtcg
    13441 gtagcgatac caatttccac cagttccggt gcacgccagg ctagaagcgc cagcaccaga
    13501 tgggagccgt tttccatcag gcagtaacca aaaatttgcc gcaggatatt gcgctggcta
    13561 acaatgcaca gcaggccaag cagaaaatga cctaacgcca ccgccagcgc tggtttcagc
    13621 ccggtagcca tcggtagctt cacgggctga acgacaaatg cgcaaagcag gacaattaac
    13681 gccgcgagca gtgccatcat tgccggaccg aataacgctt tttccgggat gttctgggga
    13741 atatttcgtg cagcgtaagt catgattaac ggtaccagca gcactttggt gataaaggcg
    13801 ctggcggacc agatcagcag ttgctctgcg gcgaacaggc acgagagggt ggcgaaaata
    13861 gacaccagca ccagcgactg gcaggcgtaa aatccgcatg acaggcgata gcttttgacg
    13921 ctaatcacaa acagcgatgt cagcatcatc agtcccgcca gattatttac gatcatagaa
    13981 ccggtcattc atcatctcct tactgcaacc aggtcgcggc gattacgctg gaaatcagcg
    14041 acatcacaat cagtacaatc agcaccaggc gcattgaccc tggcagcggt gcggcatcgg
    14101 cgacggcctc gctcggtttg ccggggacga cgcgaccaaa ccagcgaata aaccaggcga
    14161 aactggcgac cgattcaatc atcagcagaa tcatggcggg cagcaggatc cagtactcca
    14221 ctgacaacgc aaaaccggca gcaaacagcg ggaatttact gaagaagccg ttgaacggcg
    14281 gtacgccggt aatcgccagc gctgccacgc agaaacccac gcctggcaac ggcagggtgt
    14341 gcaatacgcc acgcagacgc ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg
    14401 ctacaaggaa aaacaggctt ttagcgaacg cgtggttgac gatgtaggcg atgctgccct
    14461 ccagcgccag ccgcgagccg aagatggaga gcgacaagcc gaagaacatc cagccaagtt
    14521 gagtgatggt cgaccaggcc agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa
    14581 atccataaag aatggtgacc agggccatga ccatgccaac gccgccaatc acatgcggga
    14641 tattgccgcc gtcgataatg gcgcgggcaa aaatgtaaac gcccactttc accatcgatg
    14701 cggcgtggag ataggcgctg atcggtgttg gcgcttccat tgcgtccggt agccacgctt
    14761 gcatcggtag ctgggccgat ttcccccacg cggcaaacag gatgccgcca taaaccagat
    14821 aacgtgcgtc gccgtgtaac tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa
    14881 acagcgtggc ggcggcaaga tacaacccca acgaaccgat atgggtgata agcaacgctt
    14941 ttagtgctga acgctgcgct ttatcgctct gataataact gatcaacacc caggagcagc
    15001 cgcccgtaat ttcaaaaaac aacaactgac cgagcagcgt cgaggagagt accagtcccg
    15061 ccatcgcgcc gataaacacc agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt
    15121 gttcgcgatt tttatccgtc agataacccg tggagtagat cgtgaccagc aaaccgagaa
    15181 acaccaccac aaacagaatc agcgtacaca cgcgatcaat gaccaggcca aacaatgcca
    15241 catcccccac gttgaccaac gtcaacgtga cggcaacttt atcggcctga taaaacgcgg
    15301 agatcagcga caacatgcac agcgtggtca gcgcggcgaa caaaaccccc cattcggcgg
    15361 cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat aaaaggcagc aataacgtcg
    15421 tcagagcaag attttccata ttccgtcagt gctccttaca gaccggttaa ccagaagacc
    15481 catgcaagcg cagcaaggct gaagccaagc caggtcacat ggtgaatgag taaaaaacgc
    15541 ccgcgtgcca gcgtgttttc tgcgattgag gccagtacaa aaatcagcaa aaccttgagc
    15601 agcgtaacga caagtgaagt cagcaggcag gcgagagaaa gttcttgcgc gcacccaaag
    15661 ggcagaaaca gggccacaaa cagtgctgcc atcacgacct gtttcagccc cagcccccat
    15721 ttcgccagcg ccagcccggc accggaatat tctgtcagcg ggccttcctg taattcctgt
    15781 tctgcttcag caacatcaaa gggaattttt cccatctcaa tgaagcaggc aaaaccacag
    15841 gccagtaacg ccagtacggt ggttagcggc gagttccagc ccgttgccag cgtattgctg
    15901 atcatctcga tatgcgtgga acctgctatc agcgccaata ccagcagtga gagaatgagc
    15961 attggttcga ccagaatgcc gagcgtcaac tcgcgactgg caccgactcc cgcaaacggg
    16021 cttccggtat ccagcccgga aagagcaaag aaaaaacgaa acagggcaag aagatagata
    16081 agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg taataaatag tggtagggcc
    16141 atcgccagca ccagcatgct gctgattaat acccacggca tcaggcggaa catcagacct
    16201 gaagatatag gcgcaacttc ctggcgttta aacagtttgt ggatatcgcg ataatcctgc
    16261 cagatccccg gcccgcggcg ggagtgcata cgcgcgcgta tctgccggga aatacccgta
    16321 aaaagcgggg ttagcatcag cagaatcacg gcctgtgcta acgcaaaaat gaccagatat
    16381 ccgtcgcaaa gagtttgtct catggtgatt tccttagacg gcaatagcga ttagcagcac
    16441 aaccagtgcg gcgaccacat acaggcaata gagacgaaag tcgccgcttt gcagatgctg
    16501 tatttctttg gccagccgtt gggtggcgct cacgatgggg cggatcaccc gctcatccca
    16561 gaagggttct gtgctctgag ccctggcggt gacgtgcgca agacctttat ttagcctcag
    16621 cgtagggtcg agctgttgtc gtagacgaaa tagcgcagag aagaccacac gcagcggctg
    16681 catcacgcca ttgcctgacg gggccatcgc attttcccag ccgtagccgc atgcccaggc
    16741 atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac cacagccagt aaaggccagg
    16801 cagcaaaggc atcgccagta gtaacagcaa cagtaatgag ggggtgacct gtgtatgaaa
    16861 cgtgccgggt acaagtgcta ttccgctggc gacagtgacg ggaggggtat tggtaaacgc
    16921 atgggcaata tgcattatct tcggtgccag ccaactggca ctaagcgcaa ttaatacgca
    16981 gagtgccgcg agcagtagca tggcgacgat catcgtattt ggcacttcct gtgcctcttc
    17041 agcgtgtgta ctgcgcggcg caccacagaa agtaataccg tacattttga cgaagcacat
    17101 tactgccagc ccaccagtga ctgccagcat caccatagca ataggacccg caagttgtag
    17161 cgctacggct tccacacgac ttagtgagaa cagcgactgc caggtgtacc attcgctgat
    17221 aaaaccattc agcggaggaa gggctgatat cgcgaggcaa ccaatcaggc atgctgcggc
    17281 tgtccacggc atccgtttcg ccagtgcccc cattttttcc atgtcgtggg tatgcaaacg
    17341 cgaaataatc gcgcccgcgc cgagaaatag cagccctttg aacagcgcat ggttgagcag
    17401 atgaaacagt gcgccgagca gtccaaccac ggtgagcagc gggtcgtgta gtgacagacc
    17461 gaccatcgcc acaccgactg ccagcaaaat aatgccgacg ttttcgacgg tactccaggc
    17521 cagcagccgt ttgatatcct gttccgccag cgcatacagc acgcccagga gcgcggagat
    17581 tgcgccgatc gccatcacca gaatgcccca ccacagcggc aaacccgttt gcgccagcag
    17641 atccatcgcc actttcagga tgccgaaaat accgattttg accattacgc cagacatcaa
    17701 cgccgaagcg tgcgatggtg cggcagggtg agcgcgcggc aaccagctgt gcaacggcat
    17761 catcccggct ttcgcgccaa aaccgaaaaa ggccagcagg aacaccgccg acgccagccc
    17821 cggagaaagt gaaagcgtgc ggaaactggc aaaatctagg ctgccgcttt cgcgccccat
    17881 cagcaagaag gcgatcataa tcagcaccga tccggcgtgg gcgataaaaa agtagagcat
    17941 gccagcgttg atcgacgttt tatcctgcct ggcaatgacc agaaaccagg aagacagcga
    18001 catcatttca aacagcacga tgaaccaaaa agcgttgtcc atcaccagca gggcaaccat
    18061 cgatgcgatg aaaatattca taaagaagcc catcgccgcc gcgcctttgc cctcgtattc
    18121 gcgcatataa gtcaatgaat agagcgaaca aaccaccacc agcaaggaga taaccagcac
    18181 cataaatgcc gagaggctat ccattcgcag ggttaaatcg gcaaagtcaa aaggggtggc
    18241 aaaacgggca actaaagtta cgccttcatg taattgcgta attccgctga tgatgccaat
    18301 cacgccgccc accagcgatg tgatgccgga aagcttaata gccagtctgt ccagaccgag
    18361 taaaaacagc gaagccagac tggcaaacag atagaggatc agcgaccagg ttaataattg
    18421 cagggcatcc attagcgttg ctcctgagtg agggaaaggg gatcctccct gccattggca
    18481 aaccagcttg cggcaaggcg ctgtttttct ttcatctgac gttgcaggct atcgtcggtg
    18541 atcagccgta acgcctgatt cgggcaagcg cgaacgcagg caggaccttc tggcaagaaa
    18601 tcacacaggt cgcatttcac cgcgacggtc tggacacccg gttcccagcg cagcaaagca
    18661 tgttgtgttg gcgcgttttc ttcgccgtct ttgagtgagc cttcagcctg aaaaacatat
    18721 tgcgcagggg cattcaccgg acggcttcct gaagcgctga ttgcgccaaa tgggcagacc
    18781 acggcgcaaa gcttgcagcc aatacagagg ctttcgttga gttggatcgc atcatccctc
    18841 tgggagatgg cattgaccgg gcagacctgc acgcaagggg cttcctcaca gtgatgacac
    18901 acgacagggg cagtgagtgt tgacgtcttc gccagggcca ggcgcgggtg ttgctgtaaa
    18961 ccttgcgttt tatgcacgtc cgaacaggca gcgagacagg tattacatcc tgtacaccac
    19021 agtggttcgg ccaccacaaa gcggttcatg tcggctcctt gaaaatgata tgaaacattg
    19081 catttcataa tgcataaagg atgtcagtat gattaattta atatattcaa taagttatga
    19141 attaactgaa ttctgaacgg tacaaaaatg atggtcatta acgttcagca aaaaatggat
    19201 tcgacacccg tgtcgcaggg gaaagtgaga atgtgatctt gatcacgata agtgaggtgt
    19261 gctgaccgca agcgcagcca gcacggaatg gagcaaagta atcaggcgtg ttctttcagc
    19321 cagttcagca caacgtcgtg gtgattgctg gttttgaaat cgtcaaagac atgttcgatt
    19381 ttgccatcag cgtcaatcag gaagctgatg cgatgaatac catcgtaggt tttgcccatg
    19441 aaggactttt ccccccagac gccgaattgc tcgcacacct ggtggtcctc atcagacagg
    19501 agcgtaaagt taagcagctc tttttccgca aaacgggaga gtttttcggg tttatcggtg
    19561 ctgataccca gcacatcaac gcccgctttt ttcaactcat ccatgttatc gcgtaagccg
    19621 caggcctgta cggtacagcc gggggtcatg gctttcgggt agaaataaac cagaacacgc
    19681 tgtccctgga agtcggtcaa attaacttgt tctccgtctt gatccggcaa gctaaatttc
    19741 ggtgcgatat caccggcttt cagtggattc attacttaac tccatcctgt tcatcatgtt
    19801 gggaataatt gacgacgtta atactgcctt gtgcattgag ttctgtacat agggctttga
    19861 acgcttgctc aatatttgct gcgtccgcag atgcggggct gtgggcagta atctgaatat
    19921 gcaactgcgc agcccgttca ttttcagcag gttgtgtgcg cgacaccagc tccgcaatgt
    19981 tcatatgatg cgcgtcgaaa agtgctgtga agcgttcaat taaatgcggg gagtctgcca
    20041 catcgacctg aacccagaca gatgctggca ttggcggacg cggacgcgcc gtcgtgcgct
    20101 tcatcacgat taaaagatcc agttcggcac ctttcaacgg taacgttgat tcaatcagag
    20161 taatggcatt ccatgaaccg gaaagcagca tgataaacgt gaactcttct cccagcatcg
    20221 ccaggcgact gtcttcaata ttacagccgc aactactgac atgacgggtg atggtgttca
    20281 caattccagg gcgatcggca cccaacgcag tgatcaccag ataatgttgc gatgacagtg
    20341 tcaaactggt tattccttta aggggtgagt tgttcttaag gaaagcataa aaaaaacatg
    20401 catacaacaa tcagaacggt tctgtctgct tgcttttaat gccataccaa acgtaccatt
    20461 gagacacttg tttgcacaga ggatggccca tgttcacggg aagtattgtc gcgattgtta
    20521 ctccgatgga tgaaaaaggt aatgtctgtc gggctagctt gaaaaaactg attgattatc
    20581 atgtcgccag cggtacttcg gcgatcgttt ctgttggcac cactggcgag tccgctacct
    20641 taaatcatga cgaacatgct gatgtggtga tgatgacgct ggagctggct gacgggcgca
    20701 ttccggtgat tgccgggacc ggtgctaacg ctactgcgga agccattagc ctgacgcagc
    20761 gcttcaatga cagtggtatc gtcggctgcc tgacggtaac cccttactac aatcgtccgt
    20821 cgcaagaagg tttgtatcag catttcaaag ccatcgctga gcatactgac ctgccgcaaa
    20881 ttctgtataa tgtgccgtcc cgtactggct gcgatctgct cccggaaacg gtgggccgtc
    20941 tggcgaaagt aaaaaatatt atcggaatca aagaggcaac agggaactta acgcgtgtaa
    21001 accagatcaa agagctggtt tcagatgatt ttgttctgct gagcggcgat gatgcgagcg
    21061 cgctggactt catgcaatta ggcggtcatg gggttatttc cgttacggct aacgtcgcag
    21121 cgcgtgatat ggcccagatg tgcaaactgg cagcagaagg gcattttgcc gaggcacgcg
    21181 ttattaatca gcgtctgatg ccattacaca acaaactatt tgtcgaaccc aatccaatcc
    21241 cggtgaaatg ggcatgtaag gaactgggtc ttgtggcgac cgatacgctg cgcctgccaa
    21301 tgacaccaat caccgacagt ggtcgtgaga cggtcagagc ggcgcttaag catgccggtt
    21361 tgctgtaaag tttagggaga tttgatggct tactctgttc ataagtcgcg cctggcaacg
    21421 gttgcgggtg tttcgcttgt tttattactc gctgcctgta gttctgactc acgctataag
    21481 cgtcaggtca gtggtgatga agcctacctg gaagcggcac cgcttgcgga gcttcatgcc
    21541 ccggctggaa tgattttgcc ggtgacctcc ggtgattatg caatcccggt gaccaacggt
    21601 agtggtgctg tcggtaaggc gctggacatt cgtccaccag cccagccgct ggcactggtt
    21661 tctggcgcgc gtacccagtt cacgggcgat accgcttcat tgctggtgga aaatggtcgt
    21721 ggcaatactc tgtggccgca ggtggttagc gtgctgcagg cgaaaaacta caccatcacc
    21781 caacgtgatg atgctggtca gacactgacc accgattggg tacaatggaa ccgtctggac
    21841 gaagacgagc agtatcgtgg tcgttatcaa atctctgtta agccgcaggg ttatcagcag
    21901 gcggttacgg ttaaactgct gaacctggaa caggcgggca aaccggttgc agacgcggct
    21961 tccatgcagc gttacagcac ggagatgatg aacgttattt ccgctggtct ggataaatct
    22021 gccactgacg ccgcgaacgc tgcgcaaaat cgtgcctcca ccactatgga cgtacaaagt
    22081 gcagctgatg acaccggttt accaatgctg gtcgtacgcg ggccgttcaa tgtggtttgg
    22141 caacgtctgc cagcggcgct ggaaaaagtg ggcatgaaag tgaccgacag cacccgttcg
    22201 cagggcaaca tggccgtaac ttataagccg ctgtctgaca gcgactggca ggaactgggc
    22261 gcgagcgatc caggcctggc atctggtgac tataaactgc aggttggcga tttagataac
    22321 cgcagcagcc tacagttcat cgatccgaaa ggtcatactc tgactcagag tcagaacgac
    22381 gcgctggtag ctgtcttcca ggctgcgttt agcaagtaaa aatacagggc tggaatcatc
    22441 cggccctttt ttctgatatg atacgcaaac gtgtgcgtcg gcaggaaaac gcgattttag
    22501 cggtaattcg cacgaaattt gtttgtcgga cgtagttcgg ataaggcgtt cacgccgcat
    22561 ccgacaaaac atccggcaca ccagacagca aaagatttta aaacgttaat tcacacccag
    22621 gagtgataaa gatgcaaaag caagctgagt tgtatcgtgg taaagcgaaa accgtataca
    22681 gcacggaaaa cccggacctg ttggtgctcg aattccgcaa tgatacgtca gcaggggatg
    22741 gcgcgcgcat tgagcagttt gatcgcaaag gtatggtgaa caacaagttc aactacttca
    22801 ttatgagcaa actggctgaa gcgggtatcc cgactcaaat ggagcgtctg ctctccgata
    22861 ccgaatgtct ggtgaaaaag ctggatatgg tgccggttga gtgtgtcgtg cgtaaccgtg
    22921 ctgctggctc tctggtgaaa cgtcttggaa tcgaagaagg tattgagctg aacccgccgc
    22981 tgttcgatct gttcctgaaa aacgacgcca tgcacgatcc gatggtcaac gaatcttact
    23041 gcgaaacctt tggctgggta agcaaagaga acctggcacg catgaaagag ctgacctaca
    23101 aagcgaacga cgtgctgaaa aaactgttcg atgatgctgg tctgattctg gtcgacttca
    23161 agctggagtt tggtctgtac aaaggtgaag tggtactggg cgatgagttc tccccggacg
    23221 gtagccgcct gtgggacaaa gaaacgctgg agaaaatgga caaagaccgt ttccgccaga
    23281 gcctcggtgg cctgatcgag gcctatgaag ccgtcgcccg ccgcctgggt gtacagctgg
    23341 actgattttt ctgttcatca tcttgccgtg ctgctggcac ggcaagacaa ccattctcgt
    23401 aagatgtgca ttgaacgctc attcctccgc catttcatcc cgttttcctg tggtaatctt
    23461 cctctgaacc acctacgatc atttctataa tgaatatatt gttgaggtat ctatgcgttg
    23521 gcaagggcga cgtgaaagtg acaatgttga agacaggcgc aatagctccg gcggtccgtc
    23581 aatgggcggt cccggtttcc gtctgccgag cggtaaaggc gggctgattt tactgatagt
    23641 cgtgctggtt gcaggctact atggcgttga tttaaccggg ttgatgaccg ggcagccggt
    23701 ttctcaacag caatcaacgc ggtcaattag cccaaatgaa gacgaagccg caaaattcac
    23761 ctcggtgatt ctggcaacca cggaagacac ctggggacaa cagttcgaga agatgggtaa
    23821 gacctatcag caaccgaagc tggtcatgta ccgtggaatg acgcgtaccg gatgcggggc
    23881 gggccagtcc ataatggggc cgttctattg cccggcggat ggtacggttt atatcgatct
    23941 ctccttctat gatgacatga aagacaaact tggcgcggat ggcgattttg cccaggggta
    24001 cgttatcgcc catgaagtcg gtcatcatgt gcagaaactg ttaggcatcg agccgaaagt
    24061 tcgtcaactg caacaaaacg cgacgcaggc ggaagtgaac cgcttatctg tgcgtatgga
    24121 actccaggcc gactgttttg ccggtgtctg ggggcatagc atgcagcagc aaggcgttct
    24181 ggaaaccggc gatctggaag aggcgctgaa cgcggcgcag gccatcggcg atgaccgttt
    24241 acaacagcaa agtcaggggc gagtagtacc agacagtttc actcatggca cttctcagca
    24301 acgctacagc tggtttaaac gtggtttcga cagcggcgat ccggcacaat gcaatacttt
    24361 tggtaaaagc atttaacttc cggggcaggg atggctgaac tgactgcgct tcacacatta
    24421 acagcgcaaa tgaaacgtga agggatccgc cgcttgctgg tgttgagcgg ggaagagggt
    24481 tggtgttttg atcatgcgct taagttacgt gatgccttac ctggcgactg gctgtggatt
    24541 tcgccgcagc cagatgctga aaaccactgt tctccctcgg cactacaaac tttacttggg
    24601 cgcgagttcc ggcatgcggt attcgacgcc cgccacggct ttgatgccgc tgcctttgcc
    24661 gcacttagcg gaacgttgaa agcgggaagc tggctggttt tgttactccc tgtatgggaa
    24721 gagtgggaaa accaacctga tgccgactcg ctgcgctgga gtgattgccc tgaccctatt
    24781 gcgacgccgc attttgtcca gcatttcaaa cgcgtactta cggcggataa cgacgctatc
    24841 ttctggcggc aaaaccagcc gttctcgttg gcgcatttta ctccccgtac tgactggcac
    24901 cccgcgaccg gcgcaccaca accagaacaa cagcaaatct tacagcagct actgaccatg
    24961 ccgtcgggcg tggcagcggt aactgctgcg cgtgggcgcg gtaaatcggc gctggcaggg
    25021 caactcattt ctcgtattgc gggtagtgcg attatcactg cgcccgcaaa agcggcaacg
    25081 gatgtactgg cacaatttgc gggcgagaag tttcgcttta ttgcgcctga tgccttgtta
    25141 gccagcgatg agcaagccga ctggctggtg gtcgatgaag ccgcagccat acctgcgccg
    25201 ttgttgcatc aactggtatc gcgttttcct cgaacgttgt taaccactac ggtgcagggc
    25261 tacgaaggta ccggacgtgg ttttttgctg aaattttgcg ctcgctttcc gcatttacac
    25321 cgttttgaac tgcaacagcc gatccgctgg gcacagggat gcccgctgga aaaaatggtt
    25381 agtgaggcac tggtttttaa cgatgaaaac ttcacccata caccacaagg caatatcgtc
    25441 atttccgcat ttgaacaaac gttatggcga agcgagccag aaacgccgtt aaaggtttat
    25501 cagttattgt ctggtgcgca ctaccggact tcgccgctgg atttacgccg catgatggat
    25561 gcaccagggc aacatttttt acaggcggct ggcgaaaacg agattgccgg agcgctgtgg
    25621 ctggtggatg aggggggatt atctcaagaa ctcagtcagg cggtatgggc aggttttcgt
    25681 cgcccgcggg gtaatctggt ggcccagtcg ctggcggcgc acggcagcaa tccactggcg
    25741 gcgacattgc gtggacggcg ggtcagccgg atagcagttc atccggcgcg tcagcgcgaa
    25801 ggcgttgggc aacagctcat tgccagcgct ttgcaatata ggcctggcct cgactatctt
    25861 tcggtgagtt ttggttacac cggggagtta tggcgtttct ggcaacgctg cggttttgtg
    25921 ctggtgcgaa tgggtaatca tcgtgaagcc agcagcggtt gctatacggc gatggcgctg
    25981 ttaccgatga gtgatgcggg taaacagctg gctgaacgtg agcattaccg tttacgtcgc
    26041 gatgcgcaag ctctcgcgca gtggaatggc gaaacactcc ctgttgatcc actaaacaat
    26101 gccgtccttt ctgacgacga ctggcttgaa ctggccggtt ttgctttcgc tcatcgtccg
    26161 ctattaacat cgttaggttg cttattgcgt ctgctacaaa ccagtgaact ggcattaccg
    26221 gcgctgcgtg ggcgtttaca gaaaaacgtc agcgacgcgc agttatgtac cacacttaaa
    26281 ctttcaggcc gcaagatgtt actggtccgt cagcgggaag aggccgcaca ggcgctgttc
    26341 gcacttaatg atgttcgcac tgagcgtctg cgcgatcgca taacgcaatg gcaatttttt
    26401 cactgactcc ttcaattttc tcccatggtc attgtgagtc agcggcgtaa aattaatgac
    26461 atcagttaag gagttcgcca tgaaacatga ccattttgtt gttcaaagcc cggataaacc
    26521 tgcgcaacag ttgttactgc ttttccacgg tgtcggggat aacccggtgg caatggggga
    26581 aataggtagc tggtttgcac cgttgttccc ggatgcgctg gtggtgagtg tcggtggcgc
    26641 ggagccgagc ggtaatccgg cggggcgtca gtggttttcg gtgcagggta ttacggaaga
    26701 taatcgccag gcgcgtgtgg atgccatcat gccgacgttt attgaaacgg tgcgctactg
    26761 gcagaaacag agcggagtag gggctaacgc cacggcgcta atcggtttct ctcaaggcgc
    26821 gattatggcg ctggagagca ttaaagccga accgggcctt gcttcacgcg tcattgcttt
    26881 taacggacgc tatgcgagcc tgccggaaac cgcgtctaca gccacgacaa ttcacttgat
    26941 tcatggcggt gaagatccgg ttatcgatct ggcgcatgca gttgcggcgc aggaagcgtt
    27001 aatcagtgcc ggtggtgacg tgacgctgga tatcgtggag gatttaggcc atgcgatcga
    27061 caatcgcagt atgcaatttg ccctggatca cttgcgttat accattccga aacattattt
    27121 cgacgaagcg ctaagcggcg gtaagcctgg cgatgatgat gtgattgaaa tgatgtaata
    27181 atagccgggt aggcctgata agaccgtatc aggcatcatg gccggatgcg gcgttaacgc
    27241 cttatccggc cacttagctt acttcttcgg ttgatccttt ttcggccagt cgtcgtcatc
    27301 gtcccactta tcgttgaaat cacgatgcgg gggaagctcc ggcttgttgg cgagaaattt
    27361 cttatggtcg acgcgcttga gatctttaat cacgttcagc aggacgccta ccaaaaagac
    27421 aatcaccaga atccaccaat atttagccag ccagtccatg cttatttcct cttgcagaac
    27481 cactcatcac gcgacgagct gttccatgat acgttgatac atacgggcaa gtagctgcag
    27541 gtcggcagcg ttcacacatt cattaatttt atgaatagtg gcattgaccg gcccgagttc
    27601 caccacctgc gcccccatgc gggcaataaa gcgcccgtcg gacgttccgc ctgtggtcag
    27661 tagctgcggt ttaatttcat tatagtgctc aaccgcgtta acgaccgcat ccaccagttt
    27721 accgcgcgcg gtcaaaaatg gctgcccgga aagccaccaa tccaccgtat agcgcagttg
    27781 atgtttttca agcagggcaa gcacctgcgc tttgatcatc tcatcagtca gttcggtgct
    27841 gaagcggaag ttaaactgca caaacagttc acccggaata acgttgttac tgcccgttcc
    27901 cgcctgaata ttggcaatct gcatactggt cgccgggaag aattcattgc cctgatccca
    27961 ctcaatagcc actaattcat taaggaaagg tgctgcgcga tgtaccggat tgtcagccag
    28021 atgtgggtag gcaacatgcc cctgaacgcc atgaatggtt aggttgcagg ttaacgatcc
    28081 acgacgacca tttttcacca catcacctac cacttcgata ctcgacggtt cgccgaccag
    28141 gcagtaatcg agacgctcat tacgtgccat taacgcttcg acggctttta ccgtaccatt
    28201 gtgggcactg gcttcttcat cagaggtgat cagaaatgcc agtcgccctg tatggttggg
    28261 atgttgtgcg acaaaacgtt ctgcagctac caccatcgcc gccagcgagc ctttcatatc
    28321 tgccgcaccg cgcccgaata acatgccgtc acgaatggtt ggttcaaacg gcggattgat
    28381 ccaacgatcg gcgtcgccag gcggcaccac gtcggtatgc ccggcaaagg ccaatgtttc
    28441 accttgccca cgccatgccc aaaaattctg cgtatcggca aagtccatgc gttcaacggt
    28501 aaaaccgatc gcctgcaaac gttcaatcaa caaagcctgg catcctgcgt catcaggact
    28561 cagggaaggg cggcgaataa gctgttgtgt cagctcaata accgggcacg acatagacta
    28621 cacctcatgg aaaaatttct gataactgga atcactgaaa cccagcagca taggcttacc
    28681 aggcgcgcag agcaatggac gtttgataat tgcaggcatt tcagtcatta atgccgccgc
    28741 agaggccgca tcggtgatta tattgcgggt ggtttcgtcc agtttacgcc aggttgtacc
    28801 acgggtgttg agtaacgctt cccagcctaa ttcgttgata aaaccgttca ataattcgct
    28861 gtccagccca tcgacgcggt aatcatgaaa acgatagtcg atgttattgg cttccagcca
    28921 acggcgagcc tttttaatgg tgtcacaatt tttgatgccg taaagtgtaa ccataatgaa
    28981 tcctttttcg atttatgaga ataaatactc atttaagagt gaaaacattt tgcctgcaaa
    29041 tgattatcag gaaatataac attatcgaag tattcaaatt aacagctatt aaataatatt
    29101 aaacattttt aacgtaaaca ttaagaatat tcatcttgtt atctgtctgt attttaacca
    29161 gaattaaatc agtattagat gccgtatgaa tatctcgaaa tgttgctgtg tatcacatac
    29221 aaatcatcac tgcaggacca tagtatttac ataaggtttg tcccccggag gtagtcatcg
    29281 cagcaatcga gtaaattagc ttcaccacgg tatattgttt ccgtagaata cccataatta
    29341 taagagaggt tgttatgatt gaacgtgaac tggggaactg gaaagacttt attgaagtta
    29401 tgcttcgtaa gtaattcctg agacaaagta caaacagcaa gaacccgctg agcaggttct
    29461 taatcggaaa gttgaaaaag gcgacacatt ggcatgtcgc cttttttatt gcttattccg
    29521 ggcgcggctt cagcgggaag cggcggcgca ccagcacaaa gaacagcggc acgaagtaaa
    29581 tagccagaat agtggctgaa atcatcccgc ccattacgcc agtacccacc gcatgctgac
    29641 caccggaacc ggcacccgtg ctggttgcca ttggcaatac gccgaagata aatgccagcg
    29701 aggtcatcag aatcgggcgt aaacgctgac ggcaggcgtg gagcgtcgct tcaaacaggt
    29761 cgtggccttt ttggttcatc tcgttagcaa actcgacgat caggatggcg tttttcgccg
    29821 ataaaccaat gaccgttaac aggcccacct ggaagtaaac gtcgttttcc agcccgcgca
    29881 tccaggttgc cagcagcgcg ccgattaccc ccagcggcac gaccagcatt accgagaacg
    29941 gtaccgacca gctttcatac aatgcagcca gacacaggaa taccaccagc aaggaaatgg
    30001 cgtacagcgc cggagcctgc gcgccggaaa gccgctcctg atacgacatc gccgtccact
    30061 ccagaccaaa gccgtttggt agctgcttca ctaacgattc cataatatcc atcgccgtac
    30121 cggtactgac ccccggtgcg gcttccccaa caatttcaac cgcagaataa ccgttatagc
    30181 gttccagacg cggcgagcct gtctcccagc gtgaggtcgc gaaagcagag aagggcacca
    30241 tgccgccatc tttatttcgg acataccaga gattgatgtc atctggcagc atgcgatacg
    30301 gcgcagctgc ctgcacatag actttcttca cgcgaccgcg atccataaag tcattcacat
    30361 agctcgaacc ccaggcggtt tgcagtgtgt cgttaatatc gtcgatagca acgcccagcg
    30421 cctgagcttt acgctggtcg atatcaatct gcaactgcgg actgtcatcg aggccgttat
    30481 ggcgcacacg ggttagctcc gggttttccg ccgccagcgc cagcaactgg ttacgtgctg
    30541 ccatcagcgc atcgtgacct gctccagcgt ggtcctgcaa ctccatatca aaacctgcag
    30601 aactaccaag accgctaatt gctggcgggc tgctggcgat aacgcgagct tctttaattt
    30661 ggttaaacgc ctttgttgca cgctcgataa tggcaaacga ggtgccggtc ttactgtcgc
    30721 gttcgctcca gtctttcagg cggataaaca ttcgcgccac gttttgcccg ttacccccag
    30781 ggccagaacc aacagtggca aacaccgaca tgatgttgtc tttttcatgg gtgaagtaat
    30841 atttctcgat ttgctcaacg actttcaggg tctgttgttg ggttgaaccg ctgggcaatt
    30901 gtaccgaggt agtaaacatg ccacggtctt ccagcggtaa gaacgacgtc ggcaaacgca
    30961 ggaacaggaa caccatgccg ccaagcagca ggacataaat cacaatccag cgcaggctac
    31021 ggtggagaat tttcgccacc cctttttcgt agcgttcggc gttgcggtta aacatctggt
    31081 taaaccaggc aaaaaagcct ttttgcccgt gatgctcacc ttttttcagt ggcttaagta
    31141 gtgtggcaca cagagccgga gtgaggatca tcgctaccag tactgacagc accatcgccg
    31201 caacaatggt aatagagaac tggcgataga tggcaccggt ggtgccgccg aagaaggcca
    31261 ttggtacaaa taccgccgaa agcaccatcg caatcccgac cagtgccccc tggatctgcc
    31321 ccatcgattt acgcgtggct tcgcgaggag tgagtccttc ctcactcata atacgttcga
    31381 cgttttccac caccacgatg gcgtcatcca ccagcagacc gatcgccagc accatcgcga
    31441 acatggttaa ggtgttgacg ctgtaaccga aggcgtaaag tacggagaag gttcccatca
    31501 acaccaccgg cacggcgata gtgggtatca gcgtggcgcg gaagttttgc aggaacagat
    31561 acataacgag gaaaaccaga gcgatagctt ccagcagcgt tttcaccacg tcttcaatgg
    31621 aggctttaac aaacgaggtg gtttcatacg ccaccttgta ttccagtccg tgcgggaaat
    31681 actgcgccag ctcgtcgaga cgattgagca ccagctccgc tgtcgccatt tcgttcgcgc
    31741 cggaggccag ttttaccccc aacccggagg ctggcttacc attgaagcgg ctaagataat
    31801 cgtatttctc cgcccccatt tcggcggtgg cgacatcgcc gagccttact tctgagccgt
    31861 cctgattcac ccgcaaggtg atatcgcgga actgttctgg tgtttgcagc agtgactggg
    31921 cgttaatggt ggcgttgagc gcctgcttat cgacggaagg tgtaccacca agttgcccaa
    31981 ccgcaatctg cgcgttctgt gactcaatgg catcagtgac atctttagct gtcatctgga
    32041 aactgttgag tttcgccggg tccagccaga tacgcatgga atattgcgaa ccataggcat
    32101 cgatatcccc gacgccattt acgcggctta acgggtcctg aatattactg gcaacataat
    32161 cagcaatatc ctgtttatcc atcgaaccat cggtagagac gaaggcaatg gtcagaatgt
    32221 tggtatcgcc ggttttacgc acagtcacgc cctgattttg caccgcctgc ggtaactttc
    32281 gcatggctga ttgcagctgg ttttgtactt gctgcacggc ttcgtccgga tcggtgcctg
    32341 ctttaaaact taaagtgaca gatgcctgac cggtgccact gctctgagat gacatataca
    32401 tgagattatc gaggccggtc atattttgct cgataacctg ggtcacggtg ttttccagcg
    32461 tctgggccga tgcgccggga tagttagcgg tcactcgcac attcggtggc gcgagatcgg
    32521 ggtattgttc aacgggcaat gaaaaaatcg ccagggtacc tgtcagacac aacaggattg
    32581 ccagcaccca ggcaaaaatg gggcgatcaa taaagaaatt cgccattaaa agaggacctc
    32641 gtgtttctgc gtatcgttgt aggcttgact tgcttcactg tagcggcaag gtacgagcca
    32701 atcgtggaga aaaaaaggag ttaatgtaaa ttatcatgcc cttttatact gaaccttaac
    32761 tgcaagtatt ctttggtcag taggaggcat tacatcaact gactttcctc accctccgca
    32821 gagcgaaagc taatactcac taacgtgccg ccgccggaag gttgtgaaaa agtcagcgtc
    32881 ccacctagcc gttccgcgcg ttcgcgcatg atattcagac cataatgacc ttcgggttct
    32941 ttcggttcac cgataccaat cccgttatca cggatataaa ccgtgtgatt gccgtccggc
    33001 gcggtgacgc aactgacggc gatttcgctg gcgttggcgt gcttcatcgc attcagcacc
    33061 gcttcgcgaa taatttgcaa caaatgcacc tgcatttgcg catccagtgc cagggttggc
    33121 agacggcagt cgagggtcag tttggcgctg gtttgatttt gtaacgtatc cagcatttcc
    33181 ctcaacgcgg aggggagatc cgcctgctgc agcgtcaggc gaaaagtagt caacagctcg
    33241 cgtaactgcc gataagcatc attcaacgcc tgggaaaaat cggccatgat actttgtgcg
    33301 gtggcgttat cttccggtat cgaacgctta agtaacgtca actggatacg taagtaagaa
    33361 agtacctgag ccagcgagtc gtgcaattcg cgggcgatgg ttgcacgttc ttccatcaac
    33421 aataactgct gaaaatgctt ctgcgcctga ttaaagtaca aaccgcgtcc cagcatcgac
    33481 gaaacgctgt taagcagcgg ttctgatgat gaaacgtgac tattttgcca gtgcagttcg
    33541 ccgtaaaccg tctcttgcat tgtcaccggt aaaatctgca tcggcaattc cgggtttggt
    33601 tgcccttcgc taatccgcca gttttcaccg acatttaact ccagatattc agccgcttca
    33661 ttgtcgcgaa caatctgcaa aatatggcgg aaacaatgca catcaatctg gctggtgttc
    33721 agcgcctgcg aacattgata caacacctcc agacgacgct tggcctcgtg gagatcgcgg
    33781 gtcttttctt ctactgacgc ctccagcgaa cggtacaatt tatgcagctc gctcgacatc
    33841 tggttaaagg tttttgcaag tagaccaagc tcattcggca ggctggtatc cagcggtggc
    33901 gagtcgaact gtccgtgttc aatacgctga ctggcggtaa ccagctgatt cagcggggca
    33961 accacctgat ggcgtatgcg gcccaaagta aaaaagacca gcgtgaaaat accgataccg
    34021 ccagccaggg aaatcgccac caccagcagc attttgcgtt cagcgtagtg ctgtaaagcc
    34081 agcacgaaca ggtctatctg attaacataa ttattaatat tggcctgata ccacggcaaa
    34141 tcgcccttgc tgagccgatt attcatctcc agccagttgg cattcagatg cgcatagcga
    34201 gtttttactg cttctggcac ataccagacg ttgaggttgg ttaataccgg tgaatgcagt
    34261 gcctgctgaa acagctggcg atgtgcattg agttgtggag tgccactttg caagtcgtag
    34321 cccaggcggt aactctgcat acgcagcgat ccggcaatat tgatagcctc agcgtcgcgc
    34381 aaactgctcg ccagagttag cagagcgata cccgtggaaa gaatcgacag cagcacaatg
    34441 taaaaaaagg cccgggccag actggccgag acgggtcgtt taacaatcac acgcgtcttc
    34501 tccacaaaaa atcattaatg ttccagttcg ctgaaacgca atttagttag tgatgggatt
    34561 atttgatctg ccacagttta tagcgaaata gttacttatt ctgggcacat gaacattgcc
    34621 gattcccctg taagtcgggt aataacaaca ctcatataaa gaataaggtt tttacaacca
    34681 aaaaagaagg tcgttatgaa tcgttttatt atggccaaca gtcagcaatg tctgggttgt
    34741 catgcttgtg aaatcgcttg tgtcatggct cacaatgatg agcaacatgt cctgagccaa
    34801 caccattttc atccccgaat tacggttatc aaacatcagc agcaacgtag tgcagtgacc
    34861 tgtcaccatt gtgaagatgc gccctgcgcc cgtagctgcc ctaatggcgc aatcagccac
    34921 gttgatgaca gcattcaggt caatcagcaa aagtgtattg gctgtaaatc ctgcgtggtg
    34981 gcctgtccgt ttggtacgat gcaaatcgtc ctgacacccg tcgcagcggg caaagttaaa
    35041 gccacggcgc ataaatgcga cctttgtgca gggcgcgaaa acggtcctgc ctgtgtcgag
    35101 aattgcccgg cggacgcgct gcagctggtc actgacgccg cactctcagg catggcgaaa
    35161 tcccgccgct tgcgtaccgc acgtcaggaa catcaaccgt ggcatgccag taccgctgca
    35221 caagaaatgc cggtaatgag taaagtcgaa caaatgcagg caacgcccgc gcgtggcgag
    35281 ccggataaac tggcgattga agcgcgcaaa accggttttg atgaaattta tctgccattc
    35341 cgtgccgatc aggcacaacg ggaagcctcg cgctgcctta aatgcggcga gcacagcgtt
    35401 tgtgaatgga cctgcccgct gcataaccat ataccgcagt ggattgaact ggtgaaagcc
    35461 ggaaacatcg acgccgccgt cgagctttct cacctgacca acaccctgcc ggaaattacc
    35521 ggacgcgttt gtccgcaaga ccgtttgtgt gaaggtgcct gtactattcg cgatgagcac
    35581 ggtgcggtaa ctatcggcaa cattgaacgc tacatttcag atcaggcgtt ggcgaaaggt
    35641 tggcgtcctg acttaagcca tgtcaccaaa gtggacaagc gggtggcgat tatcggtgca
    35701 ggtccggcag ggctggcctg tgcggatgtt ctgatccgca atggcgtggc ggtgacggtg
    35761 tacgatcgcc atccagaaat cggtggcttg ctcactttcg gcattccttc tttcaaactg
    35821 gataaatccc tgctggcacg tcgtcgggaa atcttcagcg cgatggggat tcacttcgaa
    35881 ctcaattgtg aagtgggtaa agatgtctct ttggattcgc ttttggaaca atacgacgcg
    35941 gtcttcgttg gcgtaggcac ttaccgttcc atgaaagcgg gtttacccaa tgaagatgcg
    36001 ccgggcgttt atgacgcgct gccgttcctc atcgccaaca ctaaacaggt gatggggctc
    36061 gaagagctac cggaagagcc gtttatcaat accgccggac ttaacgtcgt ggtactgggc
    36121 ggcggcgaca ccgcgatgga ctgtgtgcgt actgcgctgc gccacggcgc gagtaacgtc
    36181 acctgcgctt atcgtcgtga tgaagctaac atgccaggct cgaagaaaga ggtgaagaac
    36241 gcccgcgaag agggggccaa cttcgaattt aacgtccagc cggtggcgct tgagctgaat
    36301 gaacaaggtc acgtctgcgg gattcgtttc ctgcgcacgc gtcttggcga gccggatgcc
    36361 caggggcgtc ggcgtccagt gccggtggaa ggcagtgaat ttgtcatgcc agccgacgcg
    36421 gtgattatgg cgtttggctt caatccgcac gggatgccgt ggctggagtc gcacggtgta
    36481 acggtagaca aatggggccg catcatcgcg gatgtggaaa gccagtaccg ttaccagacc
    36541 accaatccga aaatcttcgc cggtggcgac gccgtgcgtg gtgcggatct ggtggttacc
    36601 gcaatggcag aaggacgtca tgcggcacag gggattattg actggctggg ggtaaaatca
    36661 gtcaaatctc actgatagcc tgcgcagaca aacccgactt cacagcgtaa tataattgtt
    36721 catttcgcgc tgtggagtcg gtatgacgca acaaatcacc ctcattaaag acaaaattct
    36781 ctccgataac tatttcaccc tgcacaacat tacttacgat ctcacgcgca aagatggcga
    36841 agttatccgc cataaacgtg aagtttacga tcgcggtaat ggtgcgacga tcctcctgta
    36901 caacgcgaag aaaaagaccg tggttctgat tcgtcagttc cgtgtcgcta cctgggttaa
    36961 tggcaatgaa agcgggcagc tgattgaaac ctgcgccggg ctgctggata acgacgaacc
    37021 ggaagtgtgc attcgcaaag aagcgattga agagacgggc tatgaagtgg gcgaagtgcg
    37081 caaattattt gaactgtata tgtcgccagg cggtgtgact gagctaatcc acttttttat
    37141 cgccgaatac agtgacaatc agcgcgctaa cgccggtggc ggtgtcgaag atgaagatat
    37201 tgaagtgctc gagctgccgt tcagccaggc gctggagatg atcaaaaccg gcgagatacg
    37261 tgacggtaag acggtgttat tgcttaacta tttgcaaatg tcacatttaa tagactgaaa
    37321 aataacaata atatttcgtt gtttattctt ggctcaatcc gacaaatccg attgagccgc
    37381 gctacctgcg caacgaagat acgacttgtg ctgtttgttt gaacttctgg ggtcgtaccg
    37441 tccatgcgct atcgcatttt ccttctcttt tttttcgctt tgttgccgac gtctttggtg
    37501 tgggcggcac cagcgcaacg ggcgttttcc gactggcagg tcacctgtaa taaccaaaac
    37561 ttctgcgtgg cgcgtaatac gggcgatcat aatggactgg tgatgaccct gagccgcagc
    37621 gccggggcgc ataccgatgc cgttttacgt attgagcgcg gcgggttgaa gtcgccggat
    37681 gcgtctgaag gggaaattgc gccacggctg ctgttagatg gcgagccgtt ggcgttaagt
    37741 ggtgacaagt ggcggatttc accatggtta ttagtaaccg atgatacggc aaccatcacc
    37801 gcgtttttgc agatgattca ggaagggaag gcgatcacct tacgcgacgg caatcagacc
    37861 atttctctga gtgacttaaa agcggcgttg ttgtttattg atgctcagca aaagcgcgtt
    37921 ggcagtgaaa ccgcgtggat caagaaaggg gacgaaccgc cgctcagcgt accgcccgcg
    37981 cctgcgctga aagaggtcgt ggtggttaac ccaacgccga cgccactctc actcgaagaa
    38041 cgcaacgatt tgctggatta tggaaactgg cggatgaatg gtctgcgctg ctcgcttgat
    38101 ccattgcgtc gtgaggtgaa tgtcactgcg ctgactgatg ataaagcgct gatgatgatt
    38161 agctgtgagg caggggccta taacaccatt gatttggcat ggattgtgtc gcgtaaaaag
    38221 ccgctggcct cgcgcccggt ccggctgcgt ttgccgttca acaacggtca ggagacgaat
    38281 gaactggaac tgatgaacgc aacatttgat gagaaatcgc gtgaactggt gaccttagcg
    38341 aaagggcgcg gattaagcga ttgtggcatt caggcgcgct ggcgctttga cggtcagcgt
    38401 tttcgcctgg tgcgttacgc cgcagaaccc acctgcgata actggcatgg gccagacgcc
    38461 tggcccacgt tgtggatcac ccgttaaaca aaatggcggg acatgtaggc cggataagac
    38521 gctgtaggcc ggataagacg cgtcagcgtc gcatccggca atcagcatcc ggcgatcacc
    38581 atcaggcacc ttttactccc agcaccttat gcgcttttgc cacaatattc tcggcggtaa
    38641 agccaaagaa cgggaacagc ttatccgccg gagcagattc cccataaccc gtcatcccga
    38701 caattgcccc tttcagacca acatacttgt accagtaatc ggcaatacct gcttccaccg
    38761 ccacgcgagc cgcaacgtta gaaggcaaca ccgactcccg atattcctca tcctgggcgt
    38821 cgaaaatatc ggtcgagggc agggaaacca cgcgaacatt gcgaccttct cccgctaatt
    38881 tctccgccgc ttgcagggtg atttccatct ctgaaccggt ggcaatcaaa ataatatctg
    38941 gcttaccgcc gctgtctttc agcacataac cgccacgagc aatctcttta acctgatccg
    39001 gcgtacgttc cacctgggcc agattctgcc tggagagaat cagtgccgtc ggtccgttgt
    39061 ggcgctcaac cgccagcttc cagcccaccg ccgcttccac ctgatcgcac ggtcgccagg
    39121 tgctgaaatt tggcgttaag cgcaggctgg ccagttgctc aacagcctgg tgcgtcggac
    39181 catcttcgcc cagaccaatt gagtcgtggg tataaaccat aatctgccgc gctttcatca
    39241 gtgccgccat ccgcgcggcg ttacgggcgt attcaacaaa catcaggaac gttgcggtat
    39301 acggcacaaa gccgccgtgg tgcgcaatgc cgttggcgat agcggtcatg ccaaattcac
    39361 gcaccccgta gtgaatgtag ttgcccgccg gatcttcctt cagcgaaaca gaacctttcc
    39421 agatggtcag gttgctggga gccagatccg ccgaaccgcc gagcagctcc ggtagcatcg
    39481 gcccgtaagc gttaagcgta ttttgcgaag ccttacgggt agcgattttc gccggattcg
    39541 cctgcaactc attgatatat ttctgagtcg ttttctccca gtccttcggt aaaccaccgc
    39601 tcatccgacg ggtaaactct tctgccagtt gcggatgagc ctttttatag gcggcaaact
    39661 tctcattcca gctctgctgc gctttttcgc ctttttcgcg ggcatcccaa gcgtgataaa
    39721 tatctttagg gatctcaaat ggcggatggt gccagcccag tttttgccgt gccagcgcca
    39781 cttcttcttc ccccagcggt gcgccgtgcg cctcttcctt acctgcttta ttcggcgaac
    39841 caaagccaat caccgtacgg cagataatca gcgacggctt atctttcacg ctttgcgctt
    39901 caaggattgc ttccttcacc gcctgcggat cgtgaccgtc gatttcatgg atcacatgcc
    39961 agtgataggc ttcaaaacgt tttgccgtat cgtcggtaaa ccagccttcg gtttctccat
    40021 caatcgaaat accgttgtga tcgtaaaaac caatcagctt gcccagtccc agcgtgcccg
    40081 ccagcgaaca gacttcgtgg gaaatacctt ccatcaggca gccgtcgccc ataaacacat
    40141 aggtgaagtg atcgacaatt tcatgatccg gctggttaaa ctgcgccgcc agtgtacgtt
    40201 ccgctatcgc cagcccgacg gcgttcgcca aaccttgtcc aagaggaccg gtggtggttt
    40261 caactccggg cgtatagccg atttccgggt ggccaggggt tttcgaatgc agttgacgaa
    40321 agttcttcag ctcttccagc ggcaggtcgt aaccggtcag atgcagcaaa ctgtagagca
    40381 gcatcgacgc gtgaccgttg gaaagaataa agcggtcgcg atcataccag gttgggtcgg
    40441 tagggttatg tttaagaaaa tcgttccaca gcacttcggc aatatcagcc atgcccatcg
    40501 gcgcgccggg atgaccagaa ttggcttttt gtaccgcatc catactgagt gcgcgaatcg
    40561 cattggcaag gtcttttcgg gacatataac actccgtggc tggtttatag tttggcggca
    40621 agaagatctt ccagtttgcg ttgatcaacg gcgaacagac gaatgccttc agacagtttt
    40681 tctaccgcca tcgcatcctg attgtgctcc caacggaact ccgcttcgct catgggagct
    40741 gggcgtggga acgtctgaga aggtgggatt aatttacgta ccactggcga aactttttcc
    40801 tgcagctcct tcagcaaatt cggtgcgata gtcagtcgat cgcagccggt taaggcgagg
    40861 atttgttcgg tgcgacggaa gctcgcgccc atcacaatgg tttcatagtg gtgttgctta
    40921 tagtagtcgt agatattgcg caccgattta acgcccggat cttcttccac cacatatggg
    40981 tccatcggct tgcgtgcctg ataccagtca taaatacgcc cgacaaacgg cgaaatcaga
    41041 aaaacgcccg cttctgcaca ggcccgcgcc tgtgcaaaag aaaacagcag cgtcaggttg
    41101 cagttaatac cttctttttc cagctcttct gccgcgcgaa ttccttccca ggtcgaagcc
    41161 agtttgatca gaatgcgtga tttctcaacg ccttgttgct gatacaagtc caccagatgg
    41221 cgcgccttct caatactctt ttctttatca aaagagaggc gtgcatcaac ttctgttgac
    41281 acgcgaccgg gtacgatttt gaggatttca gcaccgaaat tgaccgccag tttgtcacac
    41341 gctgcgacca cctgttgttc ctgggtcttg ccattttttt taccccaggc gatagcatcg
    41401 tctattaaat gctcatattg tgataatccg gcagccttga gtaacagcga aggattggtg
    41461 gtggcatcct ggggatgata atggcgaatg gactcaatat cgccgctgtc tgccacgaca
    41521 gtggtgaact gtttgatgcc gtctaactcg ttcataggaa atactccttg aaaagtaaag
    41581 tgttagatga gtgcgttaat tcacacttct gagaaatttc gctaaacgca tcaaaaaagc
    41641 atagcagaca ggcatggtat tgctggatta agcaggtaac atcagtgtta taggattatt
    41701 accaaaacat tatattaatg gtgagagttt ggacttgctc aaagtctgta gactccggca
    41761 aggtaataat gtgcgccacg ttgtgggcag gggctgttgc ccacacactt tatttgtgaa
    41821 cgttacgtga aaggaacaac caaatggatg accagttaaa acaaagtgca cttgatttcc
    41881 atgaatttcc agttccaggg aaaatccagg tttctccaac caagcctctg gcaacgcagc
    41941 gcgatctggc gctggcctac tcaccaggcg ttgccgcacc ttgtcttgaa atcgaaaaag
    42001 acccgttaaa agcctacaaa tataccgcgc gcggtaacct ggtggcggtg atctctaacg
    42061 gtacggcggt gctggggtta ggcaacattg gcgcgctggc aggcaaaccg gtgatggaag
    42121 gcaagggcgt tctgtttaag aaattcgctg ggattgatgt atttgacatt gaagttgatg
    42181 aactcgaccc ggacaaattt attgaagtcg tcgctgcgct cgaaccgacc ttcggcggca
    42241 tcaacctcga agacattaaa gcgccagaat gtttctatat tgaacagaaa ctgcgcgagc
    42301 ggatgaatat tccggtattc cacgacgatc agcacggcac ggcaattatc agcactgccg
    42361 ccatcctcaa cggcttgcgc gtggtggaga aaaacatctc tgacgtgcgt atggtggttt
    42421 ccggcgcggg tgctgcagca atcgcctgta tgaacctgct ggtagcgttg ggtctgcaaa
    42481 aacataacat cgtggtttgc gattcaaaag gcgttatcta tcagggccgt gagccaaaca
    42541 tggcggaaac caaagccgca tatgcggtag tggatgacgg caaacgtacc ctcgatgatg
    42601 tgattgaagg cgcagatatt ttcctcggct gttccggccc gaaagtgctg acccaggaaa
    42661 tggtgaagaa aatggctcga gcgccaatga tcctggcgct ggcgaacccg gaaccggaaa
    42721 ttctgccgcc gctggcgaaa gaagtgcgtc cggatgccat catctgtacc ggccgttccg
    42781 actacccgaa ccaggtgaac aacgtccttt gcttcccgtt catcttccgt ggcgcgctgg
    42841 acgttggcgc aaccgccatc aacgaagaga tgaaactggc ggcggtacgc gcgattgcag
    42901 aactggccca tgcggaacag agtgaagtgg tggcttcagc gtatggcgat caggatctga
    42961 gctttggccc tgaatacatc attccaaaac cgtttgatcc gcgtctgata gtcaagattg
    43021 ctcctgcggt cgcgaaagcg gcgatggagt cgggcgtggc gacgcgtccg attgctgatt
    43081 tcgacgtcta catcgacaag ctgactgagt tcgtttacaa aaccaacctg tttatgaagc
    43141 cgattttctc ccaggctcgc aaagcgccga agcgcgtagt tctgccggaa ggggaagagg
    43201 cgcgcgttct gcatgccact caggaactgg taacgctggg actggcgaaa ccgatcctta
    43261 tcggtcgtcc gaacgtgatc gaaatgcgca ttcagaaact gggcttgcag atcaaagcgg
    43321 gcgttgattt tgagatcgtc aataacgaat ccgatccgcg ctttaaagag tactggaccg
    43381 aatacttcca gatcatgaag cgtcgcggcg tcactcagga acaggcgcag cgggcgctga
    43441 tcagtaaccc gacagtgatc ggcgcgatca tggttcagcg tggcgaagcc gatgcaatga
    43501 tttgcggtac ggtgggtgat tatcatgaac attttagcgt ggtgaaaaat gtctttggtt
    43561 atcgcgatgg cgttcacacc gcaggtgcaa tgaacgcgct gctgctgccg agtggtaaca
    43621 cctttattgc cgatacctac gtcaatgacg aaccagacgc agaagagctg gcggagatca
    43681 ccttgatggc ggcagaaact gtccgtcgtt tcggtattga accgcgcgtt gctctgttgt
    43741 cgcactccaa ctttggttct tctgactgcc cgtcgtcgag caaaatgcgt caggcgctgg
    43801 aactggtcag ggaacgtgcg ccagaactga tgattgatgg tgaaatgcac ggcgatgcag
    43861 cgctggtgga agcgattcgc aacgaccgta tgccggacag ccctttgaaa ggttctgcca
    43921 atattctggt gatgccgaac atggaagctg cccgcattag ttacaactta ctgcgcgttt
    43981 ccagctcgga aggtgtgact gtcggtccgg tgctgatggg cgtggcgaaa ccggttcacg
    44041 tgttaacgcc gatcgcatcg gtgcgtcgta tcgtcaacat ggtggcgctg gccgtggttg
    44101 aagcgcaaac ccaaccgctg taattctttt ttaactctca cgcttaccct gaatattcag
    44161 ggtaagcggt ttagctgcaa tatattagta aagcttatta ctgagtttgc gaataataaa
    44221 aaaagcagta tataaaatat ctcgatatta ttttatttat attcatgcgt cgcatatgaa
    44281 agtttattca taacagtaaa tatctctcca ttcttagtga tctacctcac cttttaaacg
    44341 cgcttgccga attttgttat ttactctgac gaaaaattgt cacgatacac gaaagttttt
    44401 cacaggcggc gactcatgga taaagaacgc atcattcagg aatttgtgcc cggcaaacag
    44461 gtcacactgg cgcatctcat cgcgcatccc ggcgaggaac tggcgaagaa aatcggcgtt
    44521 ccggatgcgg gcgcaattgg cattatgacg ctaactcccg gcgaaacggc gatgatcgcc
    44581 ggtgatttag cgctgaaagc cgccgatgtg catatcggat ttctcgaccg cttcagtggt
    44641 gcattggtga tttatggctc ggtgggcgca gtagaagaag cgttatcaca aaccgtcagt
    44701 ggtttaggtc gcttattaaa ttatacgctt tgtgaaatga ccaaaagtta atacgaggca
    44761 ttcatgaaac gaattgcgtt tgtgggtacc gtcggggcag ggaaaacaac gttatttaat
    44821 gcgttgcagg ggaattatac cctcgccaga aaaacacagg ccgtggaatt taatgataag
    44881 ggcgatattg atacgccggg tgaatatttt agccatcccc gctggtatca cgccttaatt
    44941 accacgctac aggatgtgga catgctgatc tatgtccacg gcgcgaatga cccggaaagt
    45001 cggttgcctg ccgggttgct ggatattggc gtcagcaaac ggcaaatcgc cgtcatcagt
    45061 aaaacggaca tgccggatgc tgacgtcgcc gcaacgcgaa agttattgct tgagacgggc
    45121 ttcgaggagc cgattttcga gctaaacagc cacgacccgc aaagcgtgca gcaactggtg
    45181 gattatctgg catctctcac caaacaggag gaagcaggtg aaaaaactca tcacagcgaa
    45241 tgatattcgt gaagcacacg cgcgcggcga acaggcaatg tccgtggttc tgcgcgccag
    45301 cattattacc ccggaagccc gtgaagtggc ggatctactg ggttttacca tcaccgaatg
    45361 tgatgaattg atcccggtaa cggcgtccgt gcccgccagc gtgccagccg ataaaaccga
    45421 aagccagcgc atccgcgaaa ccattatcgc ccagctaccg gaaggccagt ttaccgaaag
    45481 cctggtcgcg cagctgatgg aaaaagtgat gaaggaaaaa cagtcgctgg agcagggtgc
    45541 aatgcagccg agcttcaaat cggtgaccgg caaaggcggc atcaaagtga ttgatggtag
    45601 cagcgtcaag tttggtcgct ttgatggcgc acaaccgcat tgcgtgggct tgactgatct
    45661 tgtgaccgga gacgacggca gcagcatggc cgccggtttt atgcagtggg agaacgcatt
    45721 cttcccgtgg accctgaact acgacgaaat cgacatggtg ctggaagggg agctccatgt
    45781 tcgccacgaa ggcgaaacca tgatcgctaa agcaggcgac gtgatgttta tcccgaaagg
    45841 ctccagcatc gaatttggta cgacatccag cgtgaaattc ctgtatgtcg cctggccggc
    45901 taactggcaa tccctatgaa agatttcatc accgaagcat ggctaagagc gaaccatacg
    45961 ctcagcgaag gcgcagagat ccatctccct gcggacagtc gcctgacgcc ctctgcccgg
    46021 gagttgctgg aaagccgcca tctgcgcatc aagtttattg acgagcaggg ccgcctgttt
    46081 gttgacgatg aacagcagca gccgcagccc gttcatgggc taaccagtag cgatgaacat
    46141 ccgcaggcgt gctgcgaact gtgccgccag ccggtggcaa aaaagccgga tacgctgact
    46201 cacctgtcgg cggaaaaaat ggtcgccaaa agcgatccgc gtctgggttt tcgcgcagtt
    46261 ctcgacagca ccattgcgct ggcagtgtgg ctgcaaattg aactggcgga accgctgggc
    46321 tgccaggcga ttgtcgggct ttctgacgaa gatctgcacc ggctttctca tcagccgttg
    46381 cgttatcttg gtcacgatca tctggtgccg gaagcaagcc acgggcgcga tgcggcgttg
    46441 ctgaatctgc tacgcaccaa agtgcgggaa gccgaaaccg tagcggcgca ggtgtttatc
    46501 acccgcagtt ttgaagtatt acgcccggac atcttgcagg cgctgaaccg cctttccagc
    46561 acggtctacg tgatgatgat cctgagcgta acgaagcagc cgctaacggt gaagcagatc
    46621 caacaacgac tgggggaaac gcaatgatta ttgaacgttg ttgtgaactg gcgttgcgag
    46681 cgcccgccag agtggttttt ccggatgcgt tagatcaacg tgtgctgaaa gctgcgcaat
    46741 atttacatca acaaggtctg gcaacgccca ttctggtcgc caatccgttt gaacttcgtc
    46801 agtttgcgct cagtcacggc gtggcgatgg acgggctaca ggtgatagat ccgcatggca
    46861 acctcgcaat gcgggaagaa tttgctcatc gctggctggc ccgcgcgggc gaaaaaacgc
    46921 cgccggatgc gctggaaaaa cttaccgatc cgctgatgtt cgccgccgca atggtcagcg
    46981 ccggtaaagc ggatgtctgt atcgcgggca acctctcttc cacggcgaat gtgctgcgtg
    47041 ccggattacg cattatcggc ttgcagccag gctgtaaaac gctctcatcc attttcctga
    47101 tgctgccaca gtacagcggt ccggcgttgg gctttgccga ttgcagcgtg gtaccacagc
    47161 cgacggcggc gcagctggcg gatatcgcgc ttgccagcgc cgaaacctgg cgcgccatca
    47221 ccggagaaga gccgcgcgtg gcgatgctgt cgttttccag taacggtagc gcccgtcacc
    47281 cctgcgttgc caatgtccag caggcgacag aaatcgtccg tgagcgcgca ccaaagctgg
    47341 tagtcgatgg cgagttgcag tttgacgccg ccttcgtgcc ggaagtggcg gcgcaaaaag
    47401 cgcctgccag cccgctacag ggcaaggcca atgtgatggt ttttccgtcg ctggaagccg
    47461 gaaatattgg ctacaaaatc gcacaacgac tcggcggata tcgtgccgtc gggccattga
    47521 tacaaggact tgccgcgccg atgcacgatc tctctcgtgg ttgtagtgtg caggaaatta
    47581 tcgagctggc gctggtggca gctgtgccgc gtcagacaga agtgaaccgc gaaagcagtt
    47641 tacaaacact ggttgaatga acggtcccgt tctggaccct gataaagagg aaagcacaat
    47701 ggaagcatta ggaatgatcg aaacccgggg cctggttgca ctgattgagg cttctgacgc
    47761 gatggtgaaa gctgcgcgcg tcaagctggt tggtgttaag cagattggcg gcggcctgtg
    47821 tactgccatg gttcgtggtg atgttgcagc ctgtaaagcg gcgaccgatg ccggtgccgc
    47881 agcggcacag cgtatcggcg agctggtatc tgtacacgtt atcccgcgtc cgcacggcga
    47941 cctggaagaa gtgttcccga tcggcctgaa aggcgatagc agcaacctgt aatcaccctg
    48001 tgtgacgtcg attcgggcgt cacgctctct catttttcat tttaccttcc gcggagggta
    48061 gggggagctt ttacctgaaa tacgccacgg aggcgggtat gaaactggca gtcgtcactg
    48121 gacaaattgt ttgtaccgta cgccatcacg gactggcgca tgacaaattg ctgatggtgg
    48181 aaatgattga tccacatggt aatcctgacg ggcaatgtgc cgtcgccatc gacaatattg
    48241 gcgcgggaac cggggagtgg gtgttgctgg tgagtggcag ttccgcccgc caggcgcata
    48301 aaagcgaaac gtcaccggtc gatctgtgcg tgattggcat cgtcgatgag gtggtgtctg
    48361 gcggtcaggt aattttccac aaataaggca gaacatcatg aatcaacagg atattgaaca
    48421 ggtggtgaaa gcggtactgc tgaaaatgca aagcagtgac acgccgcccg ccgccgttca
    48481 tgagatgggc gttttcgcgt ccctggatga cgctgttgcg gcagccaaaa ttgcccagca
    48541 agggttaaag agcgtggcaa tgcgccagtt agccattgct gccattcgtg aagcaggcga
    48601 aaaacacgcc agagatttag cggaacttgc cgtcagtgaa accggcatgg gtcgcgttga
    48661 agataaattt gccaaaaacg tcgctcaggc acgcggcacg ccaggcgttg agtgtctctc
    48721 tccgcaagtg ctgacgggcg acaacggcct gactttaatt gaaaacgcgc cctggggcgt
    48781 ggtggcttcg gtgacgccgt ccactaaccc ggcggcaacc gtaattaata acgccatcag
    48841 cctgattgcc gcgggcaaca gcgtcatttt tgccccgcat ccggcggcga aaaaagtctc
    48901 ccagcgggcg atcacgctgc tcaaccaggc gattgttgcc gcaggcgggc cggaaaactt
    48961 actggttact gtggcaaatc cggatatcga aaccgcgcaa cgcttgttca agtttccggg
    49021 tatcggcctg ctggtggtga ccggcggcga agcggtagta gaagcggcgc gtaaacacac
    49081 caataaacgt ctgattgccg caggcgctgg caacccgccg gtagtggtgg atgaaaccgc
    49141 tgacctcgcc cgtgctgctc agtccatcgt caaaggcgcg tctttcgata acaacatcat
    49201 ttgtgccgac gaaaaggtac tgattgttgt tgatagcgta gccgatgaac tgatgcgtct
    49261 gatggaaggc cagcacgcgg tgaaactgac cgcagaacag gcgcagcagc tgcaaccggt
    49321 gttgctgaaa aatatcgacg agcgcggaaa aggcaccgtc agccgtgact gggttggtcg
    49381 cgacgcaggc aaaatcgcgg cggcaatcgg ccttaaagtt ccgcaagaaa cgcgcctgct
    49441 gtttgtggaa accaccgcag aacatccgtt tgccgtgact gaactgatga tgccggtgtt
    49501 gcccgtcgtg cgcgtcgcca acgtggcgga tgccattgcg ctagcggtga aactggaagg
    49561 cggttgccac cacacggcgg caatgcactc gcgcaacatc gaaaacatga accagatggc
    49621 gaatgctatt gataccagca ttttcgttaa gaacggaccg tgcattgccg ggctggggct
    49681 gggcggggaa ggctggacca ccatgaccat caccacgcca accggtgaag gggtgaccag
    49741 cgcgcgtacg tttgtccgtc tgcgtcgctg tgtattagtc gatgcgtttc gcattgttta
    49801 aggagtaaca gatggcgcac gacgaacaat ggctcacccc acgtctgcaa acggcggcaa
    49861 cgctgtgtaa ccagacgccc gccgcgacgg aatctccgct gtggctggga gtggatctgg
    49921 gcacctgcga tgtggtgtcg atggttgtcg accgcgacgg tcagccagtg gcggtatgcc
    49981 tcgactgggc cgatgtggtt cgcgacggca tcgtctggga tttcttcggc gcggtcacca
    50041 ttgttcgtcg ccatctcgac acgctcgagc agcaattcgg tcgtcgtttt agccatgcgg
    50101 cgacttcatt tcctccaggt acggacccgc gtatttccat caacgtgctg gagtctgccg
    50161 ggctggaagt gagccatgtg ctggatgagc caaccgcagt cgcggatctg ctgcaactgg
    50221 acaacgccgg tgtagtggat atcggcggtg gcactaccgg cattgccatc gtgaaaaagg
    50281 gcaaagtgac gtactcggcg gatgaagcca ccggcggaca tcacatctct ctgactttcg
    50341 ccggaaatcg ccgtatttcg ctggaagagg cggagcagta caagcgcggt cacggtgaag
    50401 agatttggcc tacggtgaaa ccggtgtacg aaaaaatggc ggacattgtc gcccggcata
    50461 ttgaagggca gggcattact gatttatggc tggcgggcgg ctcctgtatg caaccgggcg
    50521 tggcagaact gtttcgcaaa caattcccgg cgttacaggt gcatttaccg cagcacagtt
    50581 tgtttatgac cccgctggcg atcgccagta gtgggcgaga gaaagcggag gggctatatg
    50641 caaaatgaat tgcagaccgc gctctttcag gcgttcgata ccctgaatct gcaacgggta
    50701 aaaacattta gcgttccacc ggtgacgctt tgcggtccgg gctcggtgag cagttgcgga
    50761 cagcaagcgc aaacgcgtgg gctgaaacat ctgttcgtga tggcagacag ctttttgcat
    50821 caggcaggga tgaccgccgg gctgacgcgt agcctggccg ttaaaggtat cgccatgacg
    50881 ctctggccat gtccggtggg cgaaccgtgc attaccgacg tgtgtgcagc cgtggcgcag
    50941 ttgcgtgagt caggttgtga cggggtgatc gcatttggcg gcggctcggt gctggatgcg
    51001 gcgaaagccg tggcgttgct ggtgacgaac ccggatagca cgctggcaga gatgtcagaa
    51061 accagcgttc tgcaaccgcg cttgccgctg attgccattc caactaccgc cggaaccggc
    51121 tctgaaacca ccaatgtaac ggtgattatc gacgcggtga gcgggcgcaa gcaggtgtta
    51181 gcccatgcct cgctgatgcc ggatgtggcg atcctcgacg ccgcattgac cgaaggtgtg
    51241 ccgtcgcatg tcacggcgat gaccggcatt gatgcgttaa cccatgccat tgaagcatac
    51301 agcgccctga acgctacacc gtttaccgac agtctggcga ttggtgccat tgcgatgatt
    51361 ggcaaatcgc tgccgaaagc ggtgggctac ggtcacgacc ttgccgcgcg cgagagcatg
    51421 ttgctggctt catgtatggc gggaatggcg ttttccagtg cgggtcttgg gttgtgccac
    51481 gcgatggcgc atcagccggg cgcggcgctg catattccgc acggtctcgc gaacgctatg
    51541 ttgctgccaa cggtgatgga gtttaaccgg atggtttgtc gcgaacgctt tagtcagatt
    51601 ggtcgggcac tgcgaactaa aaaatccgac gatcgtgacg ctattaacgc ggtaagtgag
    51661 ctgattgcgg aagttgggat tggtaaacga ctgggcgatg ttggcgcgac atctgcgcat
    51721 tacggtgcat gggcgcaggc cgcgctggaa gatatttgtc tgcgcagcaa cccgcgtacc
    51781 gccagcctgg aacagcttgt cggcctgtac gcagcggcgc aataaatgcc gcgtcttatc
    51841 cggtctgtgg gattgcacat gtagtgcgga taaggcttca cgcagcatcc gctaataaat
    51901 aacaactaac agggagtaaa ggcgatggga attaacgaaa tcatcatgta catcatgatg
    51961 ttctttatgc tgatagctgc cgttgacagg atcctgtcac agttcggcgg ttctgctcgt
    52021 ttcctcggta agttcggtaa aagtatcgaa ggatccggcg gtcagttcga agaaggcttt
    52081 atggcaatgg gcgcactggg cctggcgatg gtcggtatga ccgcgctggc accggtactg
    52141 gctcacgtac tcgggccggt aattattccg gtttacgaaa tgctcggcgc taacccatcg
    52201 atgttcgccg gaacactgct ggcgtgcgat atgggcggct tcttcctcgc caaagagctg
    52261 gcgggcggcg acgtagccgc gtggctatac tctgggttaa ttctcgggtc gatgatgggg
    52321 ccaacgattg tgttttccat tccggtggcg ctcggcatta tcgaaccttc tgaccgtcgt
    52381 tatctggcgc tcggcgtgct ggcgggcatt gtgaccattc cgattggttg tatcgctggt
    52441 ggtctggttg ctatgtactc cggtgttcag atcaacggcc agccggtgga attcactttc
    52501 gccctgatcc tgatgaacat gatcccggtg atcattgttg cgattctggt ggcgctgggg
    52561 ctgaaattca tcccagaaaa aatgatcaac ggcttccaga tcttcgccaa attcctcgtt
    52621 gcattgatca ccctcggtct tgccgctgcg gtagtgaaat tcctgcttgg ctgggaactg
    52681 atccccggtc tggatcctat ctttatggcc cctggcgata aacccggtga ggtgatgcgc
    52741 gccattgaag ttatcggttc tatctcctgc gttctgttag gggcgtatcc gatggtgctg
    52801 ctgctgaccc gctggtttga aaaaccgctg atgagcgtcg gtaaagtgct gaatatgaac
    52861 aacatcgcgg cagccggcat ggtggcaacg cttgccaaca acatcccgat gttcggcatg
    52921 atgaagcaga tggatacccg cggcaaagtc atcaactgcg ccttcgccgt ttccgctgct
    52981 ttcgccctgg gcgaccactt aggcttcgcc gctgccaaca tgaatgccat gatcttcccg
    53041 atgattgtcg gcaagttgat cggcggcgta acggcgattg gcgtggcgat gatgctggtg
    53101 ccaaaagacg acgcgaccgc ggctaaaacc gaagcggagg cacaatcgtg aacactcgcc
    53161 agctactgag cgtcggtatc gatatcggca ccaccaccac ccaggtgatt ttctcccacc
    53221 tggaactggt taaccgtgcg gcggtgtcgc aggtgccgcg ctacgaattc atcaaacgcg
    53281 aaattagctg gcaaagcccg gtgttcttta cccctgtcga taaacagggt ggtttaaaag
    53341 aagcggaact gaaatcgctg attttagagc aatatcaggc tgcgggcatt gcgccggaaa
    53401 gcgttgattc tggtgccatc atcatcaccg gtgaaagcgc gaaaacccgc aatgctcgcc
    53461 cggcggtgat ggcgctctct cagtcgctgg gcgattttgt cgtcgccagc gccgggccgc
    53521 acctcgaatc cgtgatcgcc ggtcacggtg ccggggcgca aaccctttct gaacaacggc
    53581 tgtgtcgggt actgaatatc gacatcggcg gtggcaccgc gaactacgcc ctgttcgatg
    53641 ccggaaaaat cagcggcact gcctgcctca acgtcggtgg tcgcctgctg gaaaccgaca
    53701 gccaggggcg cgtggtttac gctcataaac cggggcagat gattgtggat gagtgtttcg
    53761 gtgcaggcac tgacgtccgt tcgctgaccg gcgcgcagct ggtgcaggtt acccgacgga
    53821 tggcagagct gattgtcgaa gtgattgacg gaacgctttc gccgctcgcg caggcattaa
    53881 tgcaaaccgg tttgctgccc gcaggtgtta cgcccgaaat cattacgctt tctggcggcg
    53941 tgggcgaatg ttatcgccac cagcccgctg acccgttctg ttttgccgat attggcccgc
    54001 tactggcgac ggcgctacat gaccatccgc gcctgcgtga gatgaacgtg cagtttccgg
    54061 cgcaaaccgt gcgcgccacg gtgattggcg cgggcgcaca taccctttcg ctctctggca
    54121 gcacaatctg gctggagggc gtacaactgc cgctgcgcaa tttgccggtg gcgatcccga
    54181 ttgatgaaac ggatctggtg agtgcctggc aacaggcgct gattcagctg gatcttgatc
    54241 ccaaaactga cgcgtacgtg ctggcgcttc ccgcctcgct gcctgtgcgt tacgccgcgg
    54301 tactgacggt catcaacgcg ttggtcgatt tcgtcgcgcg ttttccgaat ccgcatcccc
    54361 tgctggtggt ggccgggcag gactttggta aagctctggg catgctgttg cgcccacagc
    54421 tacaacaact cccgttggca gtcattgacg aagtgattgt ccgcgcgggg gactatatcg
    54481 acattggtac gcctcttttt ggcggatcgg ttgtgccggt gacggtgaaa tcactcgcat
    54541 ttccttcctg agggaacgac ttatgaaact aaagaccaca ttgttcggca atgtatatca
    54601 gtttaaggat gtaaaagagg tgctggctaa agccaacgaa ctgcgttcgg gggatgtgct
    54661 ggcgggcgtc gcagcggcaa gctcacagga gcgcgtggcg gcaaagcagg tgttgtcgga
    54721 aatgaccgta gcggacatcc gcaataatcc ggtgattgcc tatgaagatg actgcgtgac
    54781 gcggctgatt caggacgatg ttaacgaaac ggcctacaac cagattaaaa actggagcat
    54841 cagcgaactg cgtgagtatg tgctgagcga tgaaaccagc gtggacgaca ttgcctttac
    54901 ccgcaaaggg ctgacctcgg aagtggtcgc ggcggtagcg aagatttgct ccaacgcgga
    54961 cctgatctac ggcgcgaaga aaatgccggt aatcaaaaag gccaatacca ccatcggtat
    55021 tccgggcacc ttcagcgccc gtttgcagcc aaatgacacc cgtgacgacg tgcaaagtat
    55081 cgccgcgcaa atctacgaag ggctttcctt cggggtgggc gatgcggtga tcggcgttaa
    55141 cccggtgact gacgacgtgg aaaacttaag ccgcgtgctg gacaccatct atggcgtgat
    55201 cgacaaattc aacatcccaa ctcagggctg cgtactggcg cacgtcacca cccagatcga
    55261 agcgattcgt cgcggcgcgc ctggcggact gattttccag agtatctgtg gcagcgaaaa
    55321 agggctgaaa gagtttggcg tggaactggc gatgctcgac gaagcgcgcg cagtgggtgc
    55381 agagttcaac cgtatcgccg gggaaaactg cctctacttc gaaaccggac aaggttcagc
    55441 gctatccgct ggcgctaact tcggcgcaga ccaggtgacg atggaagcac gtaactatgg
    55501 gctggcgcgt cattacgatc cgtttatcgt caacaccgtg gtcggcttta ttgggccgga
    55561 gtatctctac aacgaccgcc agattatccg tgcgggctta gaagatcact ttatgggcaa
    55621 gctgagcggc atctctatgg gctgtgactg ctgttacacc aaccacgctg acgctgacca
    55681 gaacctcaac gaaaacctga tgatcctgct cgccaccgca ggctgcaact acatcatggg
    55741 gatgccgctg ggtgatgaca tcatgctcaa ctaccagacc accgcattcc acgataccgc
    55801 cactgtgcgt cagttactca acctgcgccc gtcaccggag tttgaacgct ggctggaaag
    55861 catgggcatt atggcaaacg gtcgcctgac caaacgggcg ggcgatccgt cactgttctt
    55921 ctgatgacgc ggagataaca catcatggat caaaaacaga ttgaagaaat tgtacgcagc
    55981 gtgatggcgt caatgggaca aacggccccc gcgccgtcag aagcaaagtg cgccactacc
    56041 acctgtgcgg caccggtgac ctcggaaagc tgcgcgctgg atttaggttc tgcggaagca
    56101 aaagtgtgga ttggtgttga aaacccacat cgtgcggatg tgttaacaga actgcgtcgc
    56161 agcaccgtgg cccgcgtttg taccggtcgt gccggtccgc gtccgcgtac gctggcgctg
    56221 ctgcgtttcc tggccgatca ctctcgttcg aaagataccg tactgaaaga agtgccggaa
    56281 gagtgggtga aagcgcaggg cttgctggaa gtacgctccg aaattagcga caaaaatctc
    56341 tacctgactc gcccggatat gggccgccgc ctgtgtgcag aagctgttga agcgctgaaa
    56401 gcacagtgtg ttgctaatcc ggacgtacag gtcgttattt ctgatggcct gtcaactgat
    56461 gccatcactg tgaactacga agagatcctg ccgccgctga tggcgggcct gaaacaggcc
    56521 gggctgaaag tcggcacgcc attctttgtt cgttatggtc gcgtgaagat tgaagatcag
    56581 atcggtgaga tcctcggcgc gaaagtagtg atcctgctgg tgggcgaacg tccggggttg
    56641 ggtcagtcag aaagcctctc ctgctacgcc gtttactcgc cgcgtatggc gaccaccgtt
    56701 gaggccgatc gcacctgtat ctctaacatt caccagggcg gcacgccgcc ggttgaagcc
    56761 gcagccgtca ttgtggattt ggcgaaacgt atgctggagc agaaagcatc cggcatcaac
    56821 atgacccgat aaggaggcat catgccagct ttagatttga ttcgaccgtc ggtaaccgcc
    56881 atgcgggtga ttgcctccgt taacgccgat tttgcgcgtg aactgaaatt gccgccacat
    56941 attcgtagcc tcggtctgat ttctgccgat tctgacgacg taacctacat tgcggctgac
    57001 gaagcgacca aacaagcgat ggtagaagtg gtatatggtc gctcgctgta tgcgggcgcg
    57061 gcacacggcc cgtcaccgac tgccggtgag gtgctgatta tgcttggtgg gccaaacccg
    57121 gcggaagtgc gtgccggtct ggatgcgatg gttgcgcata ttgaaaatgg cgctgctttc
    57181 cagtgggcta atgatgcgca agatacggca ttcctggcac atgtagtttc gcgtaccggt
    57241 tcttatctct catcaaccgc cgggatcacg cttggcgatc cgatggcgta tctggtggca
    57301 ccgccgctgg aagcgaccta cggcattgat gcagcgttga aatctgccga cgtacagctg
    57361 gtgacctatg tcccgccacc gtctgaaact aactactcgg cagcattttt aaccggtagc
    57421 caggcagcgt gtaaagcggc ctgtaacgcc tttaccgatg cagtgctgga aatcgcgcgt
    57481 aatccaatcc agcgtgcgta acggaggttg ccaatgatca atgcactggg attgctggaa
    57541 gtggacggca tggtcgccgc gatagatgca gcggatgcca tgctcaaagc agctaacgtt
    57601 cgtctgctca gtcacgaagt gcttgaccca ggtcgcttaa cgctggtggt ggaaggcgat
    57661 ctggcggcgt gtcgtgctgc gctggacgca ggttgtgctg ccgcgatgcg taccgggcgt
    57721 gtcatcagcc gtaaggagat cggtcggcca gacgatgaca cccagtggct ggttactggc
    57781 tttaaccgcc agccgaagca acccgtaaag gaacccgacg cgccagttat cgtcgcggaa
    57841 tctgctgacg agttgttggc gctgttaaca tcagtacgtc agggagtgac ggcaggagaa
    57901 gtggctgccc actttggctg gccgctggaa aaagccagaa atgcgctcga acagctcttt
    57961 tctgctggga cgttacgtaa acgcagtagt cgttatcgtc tcaagcccca ttaacctgtc
    58021 ggaggtgccg ggtgtcctac aacacccggc attaacatca tgaaaaagac ccgtacagcc
    58081 aatttgcacc atctttatca tgaaccctta cccgaaaacc tgaagctcac gccgaaggtc
    58141 gaagtggata atgttcatca acgacagaca acggatgtct atgaacatgc tttaacgatt
    58201 accgcctggc agcagattta cgatcagctg catccgggca agtttcatgg tgaatttacg
    58261 gaaattctac tcgatgatat tcaggttttt cgtgaataca ccggtctggc gctgcgtcag
    58321 tcgtgtctgg tctggccgaa ctcgttctgg tttggcattc cggcgacgcg cggtgagcag
    58381 ggatttatcg gttcgcaatg tctgggaagc gcagaaatag cgacgcgccc tggtggaacc
    58441 gagtttgaat taagtacgcc ggacgattac acgatcctgg gcgtagtgct ttctgaagat
    58501 gtcatcaccc gtcaggctaa ctttttgcat aatccggatc gggtattaca tatgttgcgt
    58561 agccagtcgg cgctggaagt gaaagagcag cataaagccg cgctgtgggg ctttgtccaa
    58621 caggcgctgg cgacgttttg cgagaacccg gaaaatctcc atcagcctgc agtgcgaaaa
    58681 gtgctggggg ataatttgct aatggcgatg ggggcgatgc tggaagaagc gcagccaatg
    58741 gtgacggcgg aaagcatcag tcatcagagt taccgtcggt tgctttcccg cgcccgtgaa
    58801 tatgtgctgg aaaacatgtc cgaaccggtg acggtgctgg acttgtgtaa tcaactgcat
    58861 gtcagccgcc gcacgctaca aaacgcgttt cacgctattt taggaattgg cccgaacgcg
    58921 tggctgaaac gcattcgcct gaacgccgta cgccgcgaac tgataagccc gtggtcgcaa
    58981 agcacaacgg taaaagacgc cgccatgcag tggggattct ggcatctggg gcaatttgcc
    59041 acggattacc agcagctgtt tgccgagaag ccgtcgttga cgttgcatca gcggatgcgg
    59101 gaatgggggt gagggagtta cacccaatcc ctgaccttaa taaactcact taacgccgct
    59161 tctgggctgc catcttttgg ctgataatca tactcccagc gtaccagtgg cggcattgac
    59221 atcaggatcg actcggtgcg cccgccagtt tgcaggccaa acagagtgcc gcgatcccag
    59281 accagattga actcgacata acgaccgcga cggtagagct gaaaattgcg ctcgcgctcg
    59341 ccgtaggcca tcgctttacg tcgctctaca attggtaaat aagcgtcggt gtagcctttg
    59401 cctaccgcct gcataaaggc aaaacagtgg tcgaaatctg gcgtgtttag atcatcaaag
    59461 aacagcccgc caataccgcg ctgttcgttg cgatgtttga ggtagaagta atcgtcgcac
    59521 cactttttgt aacggggata aacgtcttta ccaaatggca ggcacaggtc acgggcggtg
    59581 cggtgccagt gaatggcgtc ttcttcaaaa ccatagaaag gggttaaatc gaagccgccg
    59641 ccaaaccacc agactggctc tgcacccggt ttttcggcaa taaaaaagcg cacattcgcg
    59701 tggctggtgg gaacatacgg attatgcgga tgcactacca gagaaacgcc catcgcctcg
    59761 aaactgcgcc cggcaagttc cgggcgatga gcggtggcgg aagcaggcat cgcctcgccg
    59821 tggacgtgag aaaagttgac gcctgcctgt tcgaaaacac caccattacg caacacccga
    59881 ctacgcccgc cgccgccacc ttcgcgctgc cagctatctt cgacaaattc tgcgccatcg
    59941 acggcggtca gctgctgaca aatcgtatcc tgaaggttga gcagaaactg tttaacctgg
    60001 tgtgcgtcgg gtttcataac ttatcgcttt ttcgaatgtg ctttctggtt gtcgaaccag
    60061 tggaaataac tgatcacgcc ttcagcaatc gctgtggcga ttttctgacg aaacgccgcc
    60121 gtgcctaaca gtcgctcttc ttccgggttg gtgataaacg aggtttccac cagcaccgaa
    60181 ggaaccgacg gcgatttcaa caccacaaac gcggcttgtt cggtgttgcg gctgtgcagt
    60241 ttatgcaccg gcttaatctt tttcagaata tgcgagccga gcgtcaggct gtttttaata
    60301 gtatcggttt gcaccagatc aaacagcact tgctgcaata gatgatcctt gtcagtcgcc
    60361 tttttaccgg caacttcatc ggcgcggttt tcgcgttcag acaggtattt cgccattgcg
    60421 ctacttgccc cacggttaga gagggcaaat accgaagcac cggcagcttt cgggttggta
    60481 aagccatcgg catgaattga cataaacaga tctgcgccat gtttatgggc gatttcaacg
    60541 cgatcgtaaa gtgggataaa cgtatcgcca gaacgcgtta aacgcgcatc aatcccatga
    60601 ttacgcaaaa tggaacggac gtttttagca atcgccagca ccacatgttt ttctttcgaa
    60661 ccgttgcgcc cgatcgctcc ggtatcaatc ccgccgtggc ctggatcgag aacaacgaca
    60721 cgtttgccgc cagatttttt agctttcggc ttgctgtgtc cgttgctggt ttttaaaggt
    60781 tcgtctttgg cgatggcttg cgacattcct gacaacgtca gggcagccaa accggctttc
    60841 agcacctggc ggcgcgaagt gagtgttttt agtggtttaa aagtgctcat acggcctgag
    60901 ttgtaataat aaagttccag atgttatatc gtatcgcgta atccgttacg accgtttgct
    60961 gttgagaatt gttttacttt tcatttcaat agttgacaac tccccattat gccacttttt
    61021 caaccgattt tcgtcagata ttggctaatt cgaccgttca cgccataatc agccttttaa
    61081 cccgcaaaaa ggtaacgaat accatggaga tacgcgtatt tcgccaggaa gatttcgaag
    61141 aggtcatcac cctttgggag cgttgcgact tgctgcgtcc gtggaacgat ccggaaatgg
    61201 acatcgagcg taagatgaac catgacgtca gtttgtttct ggtcgctgag gtgaacggtg
    61261 aagtggtcgg aacggtgatg ggcggttatg acgggcatcg cgggtctgct tattatcttg
    61321 gcgtgcatcc agagtttcgt gggcgtggga ttgccaatgc gttgcttaac cggctggaga
    61381 aaaagctgat tgctcgcggc tgcccgaaaa ttcagatcaa cgtgccggaa gataacgaca
    61441 tggtgctcgg aatgtatgaa cgcctgggat atgaacacgc cgacgtgctg agtctgggta
    61501 agcgtttgat tgaagatgaa gagtactgag tttcatcctg tccattatga tgcgcacggt
    61561 cgcctgcgtt tacccttgct cttctggctt gtgctattgc ttcaggcgcg aacctgggtg
    61621 ctgtttgtca ttgccggtgc gtcgcgcgag cagggtactg cgctgctaaa tctgttttat
    61681 cccgatcacg ataatttctg gctggggctg attcctggca ttccggcggt gctggcgttt
    61741 ttgttgagcg gtcggcgggc tacgtttcct cgcacctggc gtgtactcta ttttctgttg
    61801 ctgttggcgc aggtggtttt actttgctgg caaccgtggc tgtggctgaa cggtgaatcc
    61861 gttagcggta tcggtctggc gctggtagtg gcggatattg tggcgttaat ctggctgctg
    61921 accaatcgac gtttacgcgc ttgttttaat gaagtaaaag aataacggca ctttttggtg
    61981 aatttgcact ccaagcaacg ttattgaata accaaaggaa gtgacatgaa atcgctgcgt
    62041 ttaatgttat gcgctatgcc attgatgctg accggctgtt ccacgatgtc gtcagttaac
    62101 tggtctgccg ctaacccgtg gaactggttt gggtcgtcca ccaaagtgag cgagcagggc
    62161 gttggtgaat taacggcgtc cacaccactg caagaacaag ccattgccga tgcggttgat
    62221 ggcgattatc gcctgcgcag cggaatgaaa accgcgaacg gcaacgtggt gcgctttttt
    62281 gaagtgatga agggcgacaa cgtggcgatg gtgattaacg gcgatcaagg cacgatcagt
    62341 cgcattgacg tgctggatag cgatattcct gctgacaccg gtgttaaaat cggtacaccg
    62401 tttagcgacc tttacagcaa agcatttggc aattgccaaa aagctgatgg tgatgataat
    62461 cgtgccgtcg aatgtaaagc cgaaggcagt caacatatta gctaccagtt cagcggggaa
    62521 tggagtggtc ctgaagggtt aatgccttcg gacgataccc tgaaaaactg gaaagtcagt
    62581 aaaattatct ggcggcgtta atttgcgtct gaacaaaccg ccgccgcgaa aaaacacgta
    62641 aaatagcgct caacaatgcc acggattgcg tggcattatt attttcagga ggaacaatgt
    62701 ctcaggttca gagtggcatt ttgccagaac attgccgcgc ggcgatttgg atcgaagcca
    62761 acgtgaaagg ggaagttgac gccctgcgtg cggccagtaa aacatttgcc gacaaactgg
    62821 caacttttga agcgaaattc ccggacgcgc atcttggtgc ggtggttgcc tttggtaaca
    62881 acatctggcg cgctctgagc ggcggcgttg gggcagaaga gctgaaagat tttccgggct
    62941 acggtaaagg ccttgcgccg accacccagt tcgatgtgtt gatccacatt ctttctctgc
    63001 gtcacgacgt aaacttctct gtcgcccagg cggcgatgga agcctttggt gactgcattg
    63061 aagtgaaaga agagatccac ggcttccgtt gggtggaaga gcgtgacctg agcggctttg
    63121 ttgacggcac agaaaacccg gcgggtgaag agacgcgtcg cgaagtggcg gttatcaaag
    63181 acggcgtgga tgccggcggc agctatgtgt ttgtgcagcg ttgggagcac aacctgaagc
    63241 agctcaaccg gatgagcgtt cacgatcagg agatgatgat cgggcgcacc aaagaggcca
    63301 acgaagagat tgacggcgac gaacgtccgg aaacctctca cctgacccgc gttgatctga
    63361 aagaagatgg caaagggctg aagattgttc gccagagcct gccgtacggc accgccagtg
    63421 gcactcacgg tctgtacttc tgcgcctact gcgcgcgtct gcataacatt gagcagcaac
    63481 tgctgagcat gtttggcgat accgatggta agcgtgatgc gatgttgcgt ttcaccaaac
    63541 cggtaaccgg cggctattat ttcgcaccgt cgctggacaa gttgatggcg ctgtaagcgt
    63601 ttctgattca ttgattaaag gccaggcagt aacattactg actggccttt ttattacttt
    63661 tgctttaacg ccgcatacac ctgatcaact acccaaccat acgttgcaat cggcagctga
    63721 ccgctttcga acatattggg attcttttgc ggatcggcaa ccggcgaatg tggcttgttg
    63781 cttaacatca caatcgccat atgattcacc ggatcgataa cggtcaccgt tccggtccag
    63841 ccagtgtgac cgtaggtttg cgggcttgcc agcgtgccaa acgtcggcgt catggtggca
    63901 ttaccattca cgcgccagcc gaggccaaaa gtggcatctt ccttagagct ggtggtgaac
    63961 atcttcaccg tttccgcact gaacagctgc acatcgccat agcccccgcc gttcagcatc
    64021 gtttgcatta acaccgcaat atcgccggta ttggaaaaca aacctgcgtg cccggaaacg
    64081 ccgcccatcg aataaaaggc tttttcatcg tgcacctgac cccagtgagt ggaggtgcgg
    64141 atattcggga agtggatcac tccatcgcgg gtattgccgt tcaattccgt ggcggcaatt
    64201 tgttgcggtt taaagccttt cagcagcggg ttaaataccg tatgtgtcag gccgagcggg
    64261 cgataaatcg actcttcaac atagcggtca agcggctgac cggtgaccga ctcgacgata
    64321 aatccaagca gcatataatc gacatcactg taaatatgct tgctgccggg ctgatactct
    64381 agcggcgtgc gcttgatcat ctccagcgtc tggcctttat cctgggaata taacgcgccc
    64441 gcgaccgctt tattcggata ttgtgggtcc gccgggaaac cgccgctgtg atgcagcagg
    64501 tcagaaatcc gcagcgtgtt tttgcctttg atggtgtcat ttggactatc ggcaaacccc
    64561 gaaatatatt tcgcaatcag atcgtcagga tgcagtttgc cttcgctcat tagcttttgc
    64621 agagcgaagt tagtggcata cattttggtg tttgaggcca ggtcatacag cgtcccggtg
    64681 gtagctttta caggctgcgc catcaatacg ctgccatcgt actttttcgc cgctccccag
    64741 gctttgcgat acacaatctg attatctttt ataatcagca ggtttacgcc gggataaccg
    64801 gcgtccactt gctggctaat ccagcgatcc atctggttaa gccgttcgac gttgaaccct
    64861 gctttctctg gcgaactttc tgtcagaaca gggtattttg cggcgctgac gctacaacta
    64921 acagccagca gagaaagata gagcattgtc cgtttcatga tatcagtcca gattgacgtt
    64981 acggcagcca aaaagcgtgg tgaaaataaa cccgcaaacc catgccacca gaatcccgcc
    65041 agcgtagact gccatcgccg gaagaatgcc ttgtgctgaa gtcatcagcg gcagcgccac
    65101 cagaccagac ggcccaaagg cgctgtttaa gcccatcggc agaccccacc aggctatcaa
    65161 tccgataaac aaaccgcccg ccgcgccgcc taaacaggcg gtaataaacg gtttcatgcg
    65221 gggcagggtg acgccataaa tcaacggttc gccaacgccc agcaggccgg gaataatcgc
    65281 cccgcgtacc tgactgcgta atgcactgtg cggttgcgcc cgccagtaga gtgccagcgc
    65341 cgcgcccacc tggcccgcgc ctgccatcga aaggatggga aataagctgt tgaatccttg
    65401 gctgtccatt aacgcgaggt aaacaggaat aaagccctga tgcacgccaa ataccacggc
    65461 gatcaggaac agcccggcta aaaccgcaca accgagcgga ttactgttca ggtgcataaa
    65521 cagccacgac ataccttcga ataaccagcc gcccagcggc ataatgatca gataggccag
    65581 cgtagcggtg atcagtaggg tgattaacga ggtcagcagc atgtcgagat cgtcgggcat
    65641 aaagcggcgc accatgcctt cgatgcgggc gcaggcccac gcggcaatca acacgccgat
    65701 aatattgccg cgcggatcga tgggcagacc aaagaaatcg tgaaaaccgg cgtagtaacc
    65761 ggtggttgca gcggggttat aaccgagcaa aaagagtgcg gcaataattg cgccattgac
    65821 gcccgtgccg ccaaatgcct gggcggcgtt atagcccacc agaatcacca ggaaggtgaa
    65881 caaacctttg ctgaacacct tcataaaatt cagcgcgtcg ggtagcgtgc cctgagcatc
    65941 tgccggaacg tgcatcaccg tggcaattaa cgtcgctatc cccagcagca gaccggcggc
    66001 aataaaaccg gggataagcg gcgtgaagat ggtggcgaat ttggcgagaa attgttgcac
    66061 gccggaggtt tgtttggctt ttaactgacg tttgttctgg gcggcgattt ctgcggcatc
    66121 ctgtaccggt gcttctccca gcagctcgct catcgctttt gcggcacgat gcgctttacc
    66181 aggcccaaaa acgacctgca cctggtcact ggtcaaaatg acgcccttca cgccttccag
    66241 cgttttgata tcgggatcaa ccagtgaact gtcatgtaca cccagacgca ggcgcgtcat
    66301 acagttacca caactggcga tatttcccgg tccgccgaca cgggtaagaa tggtgttcag
    66361 aagttcactg ctgatctctt tggccatggg ttattccttg tctaaaacct gacgaataaa
    66421 gccgccgtgt tgatccaggc gttttttagc ttctgcggca tcgagatttt tcagcaccat
    66481 cacaatggcc gttttacagt tgcgctcgca ggcaattaac gctgcttctg cttgttcggc
    66541 attacatccg gtggcgtttt taacgatatt gacctggcgc acatgcagtt tttcgttggt
    66601 ggcgaccaca tcgaccatca ggttgccgaa cactttgccg gatttaatca tcagcccggt
    66661 ggaaagcata ttgagcacca gtttctgcgc cgtgcctgct ttcatccgcg aagaaccggt
    66721 gaccacttca gccccaacca ccggcgtaat agcaaactca gcggtggtgg aaacggcgct
    66781 ccccggatta caggaaattc ccactgtacg gcagccgagc tggcgtgcat attccagtcc
    66841 ggcaatcaca tacggcgtgc gaccgctggc agcaatgcca accaccacat cctgcgcggt
    66901 taatccgatg tttttcaaat cattaacacc gccttcccgg ctatcttcag cgccttccac
    66961 cgcgtgctga atggcatatt cgccgccagc aattaatcca actaccagac ccggtttcac
    67021 gccgtaggtg ggcggacatt cgctggcatc cagaatcccc agacgaccgg atgttcccgc
    67081 accgagataa atcagacgac cgccgccgct tacctgggcg tggataacat cgattgccgc
    67141 ggcgatatcc ggcagtacgc gctcaacggc aagcggtacg gttttatctt catcgttgat
    67201 aatccggcac atttccagcg ttgatacgcg gtcaatttca gccgaggcgg cgttcgagcc
    67261 ttcggtaatc atcttttcaa gttgcatcgg tggtgacctt actatttacg atcaaataat
    67321 tgcagatgta tggaatcata tatcccgaat tgacagactt aaaggaatca ttgagtctac
    67381 actcgcagag tgtgaagagc gtcataagaa cttaagcgga atttttgctg ccagcccagg
    67441 agagtggaag atgttgtacc tgacgaaaat acgcaatgcg gaaagtgaat ttaccgagaa
    67501 cgagcaaaaa attgctgact ttctgcgggc caacgtgagt gagctgaaat ccgtctcttc
    67561 gcgcaagatg gcgaaacagc tcggcatcag ccagtcgagt attgtgaagt ttgcccagaa
    67621 gctcggcgca caagggttta ctgaattgcg tatggcgtta ataggcgaat acagtgccag
    67681 ccgggagaaa acgaatgcca cggcgcagca cctgcacagt tcgattacca gtgatgattc
    67741 gctggaagtg attgcccgca aattgaatcg cgaaaaagag ctggcgctgg agcaaacctg
    67801 tgcgttgttc gactacgcgc ggctgcaaaa aatcattgag gtcatcagta aagcaccgtt
    67861 tatccagata acgggcctgg gcggatcggc gctggtgggg tgcgatctgt cgttcaaact
    67921 gatgaaaatt ggatatcgcg tggcctgcga agccgacacg cacgtacaag ccaccgtttc
    67981 ccaggcgttg aaaaaaggcg atgtacaaat cgctatctct tacagcggca gtaaaaaaga
    68041 gattgtcttg tgcgcagaag cggcacgaaa gcagggggca acggtgattg ccattacttc
    68101 tctggctgac tctccgctgc ggcggctggc gcatttcacg ctcgataccg tttccggtga
    68161 aaccgaatgg cgtagctcgt cgatgtctac ccgcacggcg caaaattctg ttaccgattt
    68221 actgtttgtt ggtcttgtgc aactcaatga tgtggagtca ttgaaaatga ttcagcgcag
    68281 cagtgagcta acacaacgcc tgaaatgatg cataaagcag cgactggatt gagattttcc
    68341 tgaattagtg agctgatccg cagtaatatt ttgtttatcc tgtattttca gagggaatgg
    68401 agtgtaacgc tctgtattaa caaggagagc attaaaatgg gtaaactcac gggcaagaca
    68461 gcactgatta cgggcgcatt gcagggaatt ggcgaaggaa ttgccagaac ttttgcgcgt
    68521 catggcgcaa acctaatctt gctggatatc tcccctgaga tcgaaaagct ggcggacgaa
    68581 ctgtgtggtc gtggtcatcg ctgtacggcg gttgtcgccg atgtgcgtga cccggcgtcg
    68641 gtagccgcag ctatcaaacg cgcgaaggaa aaagaagggc gcattgatat cctggtgaat
    68701 aacgcaggcg tttgtcgtct gggcagtttc ctcgatatga gcgatgacga tcgcgatttc
    68761 catattgaca tcaatattaa aggcgtatgg aacgtcacga aggcggtgct gccggagatg
    68821 attgcccgca aagatggtcg cattgtgatg atgtcttcag tcactggtga tatggtggcc
    68881 gatccgggtg aaacggcgta cgccttaacg aaagcggcga ttgttggcct gacaaaatcg
    68941 ctggcggtgg agtatgcgca gtctggtatt cgcgttaacg ccatatgccc tggatacgtt
    69001 cgcacaccaa tggcggaaag cattgcccgc cagtcgaacc cggaagatcc agagtcggtg
    69061 ctgactgaaa tggcaaaagc aatcccgctg cgtcgcctcg ccgatccgct ggaagtcggc
    69121 gaactggcgg ccttcctcgc atcggatgaa tccagctatt taaccggtac acagaatgtg
    69181 attgatggcg gcagcacact gccggagacg gttagcgtcg gtatctgatt cacctctgtt
    69241 tcctccctgc atttgtgggg aggatttcgt cttattccct tttcaacttc caaatcacca
    69301 aacggtatat aaaaccgtta ctcctttcac gtccgttata aatatgatgg ctattagaaa
    69361 gtcattaaat ttataagggt gcgcaatggc cgttaactta ctgaaaaaga actcactcgc
    69421 gctggtcgct tctctgctgc tggcgggcca tgtacaggca acggaactgc tgaacagttc
    69481 ttatgacgtc tcccgcgagc tgtttgccgc cctgaacccg ccgtttgaac agcaatgggc
    69541 caaagataac ggcggcgaca aactgacgat aaaacaatct catgccgggt catcaaaaca
    69601 ggcgctggca attttgcagg gcttaaaagc cgacgttgtc acttataacc aggtgaccga
    69661 cgtacaaatt ctgcacgata aaggcaagct tatcccggcg gactggcagt cgcgcctgcc
    69721 gaataacagc tcgccgttct actccaccat gggcttcctg gtccgtaagg gcaacccgaa
    69781 gaatatccac gactggaacg acctggtgcg ctccgacgtg aagctgattt tcccgaaccc
    69841 gaaaacgtcg ggtaacgcgc gttataccta tctggcggca tggggcgcag cggataaagc
    69901 tgacggtggc gacaaagcca aaaccgaaca gtttatgact cagttcctga aaaacgttga
    69961 agtgttcgat accggcggtc gtggcgcgac caccaccttt gccgagcgcg gcctgggcga
    70021 tgtactgatt agctttgaat cggaagtgaa caacatccgc aaacagtacg aagcgcaggg
    70081 ctttgaagtg gtgattccga aaaccaacat tctggcggaa ttcccggtgg cgtgggtcga
    70141 taaaaacgtg caagccaacg gtaccgaaaa agcagcaaaa gcctatctga actggcttta
    70201 cagcccgcag gcgcaaacca tcatcaccga ctattactac cgcgtgaata acccggaagt
    70261 catggacaaa ctgaaagata aattcccgca gaccgaactg ttccgcgtgg aagacaaatt
    70321 tggctcctgg ccggaagtga tgaaaaccca ctttaccagc ggcggcgagt tagacaagct
    70381 gttagcggcg gggcgtaatt aatgtttgca gtttcatcaa gacgcgtcct gccgggcttt
    70441 accttaagcc tcggcaccag tctgctgttt gtgtgcctga tattgctgct gccactctcc
    70501 gcgctggtga tgcagctttc tgagatgagc tgggcgcagt actgggaagt gatcaccaac
    70561 ccgcaggtgg tcgcggccta caaagtgacg ctgctgtcgg cgtttgtggc atcaattttt
    70621 aacggcgttt tcggcctgct gatggcgtgg attttaaccc gttatcgctt tccgggccgc
    70681 acgctgcttg atgcgctgat ggatttaccc tttgcgctgc caacggcagt cgccggttta
    70741 acgctggcct cgctcttttc tgtaaacggt ttttacggtg aatggctggc gaagtttgat
    70801 atcaaagtca cctatacctg gctggggatt gcggtggcaa tggcctttac cagcattccg
    70861 tttgtggtac gtaccgtgca gccggtgctg gaagagttag gcccggaata tgaagaagcg
    70921 gcagaaacgc ttggcgcaac gcgctggcag agtttctgca aagtggtgct gccggaactt
    70981 tctccggcgc tggtggcggg cgtggcgttg tcgtttaccc gtagtcttgg tgagtttggc
    71041 gcggtgatat ttatcgccgg gaacatcgct tggaagacgg aagtgacgtc gctgatgatt
    71101 ttcgttcgct tacaggagtt tgattacccg gcggcgagcg cgattgcttc ggtgatcctc
    71161 gcggcatctc tgctgctgct gttctcaatt aacactctgc aaagtcgctt tggtcggcgt
    71221 gtggtaggtc attaatggcg gaagtgacac aattgaagcg ttatgacgcg cgcccgatta
    71281 actggggcaa atggtttctg attggcatcg ggatgctggt atcggcgttc atcctgctgg
    71341 tgccgatgat ttatatcttc gtacaggcgt tcagcaaagg gctgatgccg gttttacaga
    71401 atctggccga tccggatatg ctgcacgcca tctggctgac ggtgatgatc gcgctgattg
    71461 ccgtaccggt aaacctggtg ttcggtattc tgctggcctg gctggtgacg cgctttaact
    71521 tccccggacg ccagttactg ctgacgctac tggacattcc ttttgccgtg tcaccggtgg
    71581 ttgccggtct ggtgtatctg ctgttctacg gctccaacgg cccgctcggc ggctggctgg
    71641 atgaacataa tctgcaaatt atgttctcct ggcccggaat ggtgctggtc acgatcttcg
    71701 tgacgtgtcc gtttgtggtg cgcgaactgg tgccggtgat gttaagccag ggcagtcagg
    71761 aagacgaagc ggcgattttg cttggcgcat ccggctggca gatgttccgc cgtgtcacat
    71821 tgccgaacat ccgctgggcg ctgctttatg gcgtagtgct gaccaacgcc cgcgcgattg
    71881 gcgagtttgg cgcggtgtcg gtggtttccg gctcgattcg tggcgaaaca ctttcgctgc
    71941 cgttacagat tgaattgctg gagcaggact acaacacagt cggctccttt accgccgccg
    72001 cgctgttgac gctgatggcg attatcaccc tgtttttaaa gagtatgttg cagtggcgcc
    72061 tggagaatca ggaaaaacgc gcgcagcagg aggaaaatca tgagcattga gattgccaat
    72121 attaagaagt cgtttggtcg cacccaggtg ctgaacgata tctcactgga tattccttca
    72181 ggtcagatgg tcgcgttgct ggggccgtcc ggttccggga aaaccacgct gctgcgcatt
    72241 atcgctgggc tggagcatca aaccagcggg catattcgct tccacggcac cgacgtgagc
    72301 cgcctgcacg cacgtgatcg taaagtcggt ttcgtgttcc agcattacgc gctgttccgc
    72361 catatgacgg tgttcgacaa tatcgctttt ggcctgacgg tgctgccgcg tcgcgagcgc
    72421 ccgaatgccg cagccatcaa agcgaaagtg acaaaattgc tggaaatggt ccagcttgcc
    72481 catctggcgg atcgttatcc ggcgcagctt tccggcggcc agaaacagcg tgtagcgctg
    72541 gcgcgtgcgc ttgctgtaga accgcaaatt ctgctgcttg atgaaccgtt tggcgcgctg
    72601 gatgcgcagg tgcgtaaaga gttgcgtcga tggctgcgtc aactccatga agaactaaaa
    72661 ttcaccagcg tttttgtgac ccacgatcag gaagaagcga ccgaagtagc tgatcgtgta
    72721 gttgtgatga gccagggcaa tattgaacag gctgacgcgc cggatcaggt atggcgcgaa
    72781 ccggcgaccc gttttgtgct cgaatttatg ggcgaagtga accgcctgca gggaaccatt
    72841 cgcggcgggc agttccatgt tggcgcacat cgctggccgc tgggctacac acctgcgtat
    72901 caggggccgg tggatctctt cctgcgcccg tgggaagtgg atatcagccg ccgtaccagc
    72961 ctcgattcgc cgctgccggt acaggtactg gaagccagcc cgaaaggtca ctacacccaa
    73021 ttagtggtgc agccgctggg gtggtacaac gaaccgctga cggtcgtgat gcatggcgac
    73081 gatgccccgc agcgtggcga gcgtttattc gttggtctgc aacatgcgcg gctgtataac
    73141 ggcgacgagc gtatcgaacc ccgagatgag gaacttgctc tcgcacaaag cgcctgatag
    73201 gttgagtgaa tgttaaacgc ccggaggcgc ttcccgcgat ccgggctttt taatggcaag
    73261 gtttgtaacc tgtaggcctg ataagacgcg caagcgtcgc atcaggcaac accacgtatg
    73321 gatagagatc gtgagtacat tagaacaaac aataggcaat acgcctctgg tgaagttgca
    73381 gcgaatgggg ccgaataacg gcagtgaagt gtggttaaaa ctggaaggca ataacccggc
    73441 aggttcggta aaagatcgtg cggcgctttc gatgatcgtc gaggcggaaa agcgcgggga
    73501 aattaaaccg ggtgatgtgt taatcgaagc caccagtggt aacaccggca ttgcgctggc
    73561 aatgattgcc gcgcttaaag gctatcgcat gaaattgctg atgcccgaca acatgagcca
    73621 ggaacgccgt gcggcgatgc gtgcttatgg tgcggaactg attctggtca ccaaagagca
    73681 gggcatggaa ggtgcgcgcg atctggcgct ggagatggcg gatcgtggtg aaggaaagct
    73741 gctcgatcag ttcaataatc ccgataaccc ttacgcccat tacaccacta ccgggccgga
    73801 aatctggcag cagactggcg ggcgcattac ctattttgtc tccagcatgg gaacaaccgg
    73861 cactatcacc ggcgtttcac gctttatgcg cgaacaatcc aaaccggtga ccattgttgg
    73921 cctgcagccg gaagagggca gtagcattcc cggcattcgc cgctggcctg cggaatatct
    73981 gccggggatt ttcaacgctt ctctggtaga tgaggtgctg gatattcatc agcgcgatgc
    74041 ggaaaacacc atgcgcgaac tggcggtgcg ggaaggaata ttctgtggcg tcagctccgg
    74101 cggcgcggtt gccggagcac tgcgggtggc aaaagctaac cctggtgcgg tggtggtagc
    74161 gatcatctgc gatcgtggcg atcgctacct ttccaccggg gtgtttgggg aagagcattt
    74221 tagtcagggg gcggggattt aagattattg cgcaggcacc gttttatccg tctgcgcaat
    74281 taacgcatgt aaaaaggctg tgcattctca tcgctcttac cggggatctt cacaatatac
    74341 ggcttcccgg taatcgatga cgacgaagca accttatcaa taaactgctc ggcagtgtca
    74401 atgcggttac gggtattgcc cagcttcaga cgcagatgag aaaccgcttc atcgcaggta
    74461 tgttcatcac cgttgcgcac aaatatcaaa tcctttttct gtgctaatcc ctccagcatg
    74521 gcgttgatgc gggcttcttc atgggcagtt aacttcgcgt aagcggggag tgtcattagt
    74581 agtgtaatga ccagacaaat gattttcttc actgaattac cttatccttg ttttgtaata
    74641 gccgatttga ctgccagaat caggtaaagt ttcggttgga atacgatgag gatcatcggc
    74701 aaggagaaaa aattaaatga gtagtttgtt gttgtttaac gataagagca gggcgctgca
    74761 ggcggatatc gtcgccgtgc agtcgcaggt ggtttacggc agcgtgggca acagcattgc
    74821 cgtgcctgct atcaaacaga acggcctgaa tgtctttgcc gtgccgacgg tattgctgag
    74881 caatacgccg cattatgaca ctttctacgg tggtgcgatt ccggatgaat ggtttagcgg
    74941 ctatttgcgt gcacttcagg agcgtgatgc gctgcgccaa cttcgtgctg taaccacggg
    75001 ctatatggga acggcatcgc aaatcaaaat ccttgccgag tggctgactg cgctacgcaa
    75061 agaccatcct gacctattga tcatggtcga tccggtgatt ggcgatattg acagcggaat
    75121 ttatgtcaaa cctgaccttc ccgaagcgta tcgacaatat ttactgccgc tggcgcaggg
    75181 aattaccccc aatatctttg agttggaaat cctgaccggt aaaaattgcc gcgatctcga
    75241 cagtgccatt gctgccgcaa aaagtctgct ttcagacaca ctaaaatggg tggtgattac
    75301 cagcgcctcc ggtaatgaag aaaatcagga gatgctggtt gtggtggtca ctgccgacag
    75361 cgtgaatgtc atttcccatt caagggtaaa aaccgacctg aaagggactg gcgacctgtt
    75421 ttgtgctcag cttatcagtg gtttgctgaa agggaaggca ttaaacgatg cggtgcaccg
    75481 cgcggggttg cgcgtactgg aagtgatgcg ctacacccag cagcatgaga gcgatgaatt
    75541 gattttgccg ccgctggcgg aagcataaaa aaatggcgcc gatgggcgcc atttttcact
    75601 gcagcaagaa ttacttcttg atgcggataa ccggggtttc acccacagtc acgctaccgg
    75661 acagtttgat cagttctttg atttcgtcca tgttggagat aacaaccgga gtcagggtag
    75721 acttggcttt ctcttccagc agcggcagat caaattcaat gacagtatcg ccaactttca
    75781 cgcgctgacc ttcttcagca atacgcttga agccttcgcc tttcagttca acggtgtcga
    75841 taccgaagtg gacgaacagt tcaacgccgc tatcagattc gatagagaat gcgtggttgg
    75901 tttcaaagat tttaccaatg gtgccgtcta caggcgcgac cattttgtta cccgttggtt
    75961 tgatagcaat accatcacca acgatttttt ccgcaaaaac gacatccggc acgtcttcga
    76021 tattgacgat ctcgccagag agcggagcaa tgatctcaat agttccggta tccttcttgt
    76081 cgtcggaaac cagagatttc agtttatcga acaaacccat gatcttctcc taagcagtaa
    76141 attgggccgc atctcgtgga ttagcagatt gttttttctt caatgaactt gttaaccagc
    76201 gtcattaact cgtccgttgt cggttgagca agagcctgct ctgctaacac cttcgcatct
    76261 tcgaagttcg tgttacggat aatcttctta atgcgcggga tagaaatggc gctcatagag
    76321 aattcgtcca gacccatccc cagcaacaga agtgtagcac gttcatcgcc agcaagctca
    76381 ccacacatgc cagtccattt gccttcagca tgagaagcat caataacttg cttgatcaag
    76441 ttcagcacgg acggtgacat tggctggtaa aggtgtgaaa tcatatcatt accacggtca
    76501 actgccagag tgtactgcgt taaatcattg gtgccgatac taaagaaatc aacttctttg
    76561 gctaaatgac gtgcaattgt tgcggcagcc ggtgtttcca ccattacgcc gatttcaatt
    76621 gactcgtcaa acgctttacc ttcgtcgcgc agttcctgtt tgtagatttc gatctctttg
    76681 cgcagtgcac gcacttcttc aacagagatg atcatcggga acataatgcg caatttaccg
    76741 aaagccgagg cacgcaggat agcgcggagc tgatcgcgca ggatctcttt acgatccatt
    76801 gcgatacgga tagcgcgcca gccgaggaac gggttctctt ctttcgggaa gttcatgtat
    76861 ggcagctctt tgtcgccgcc gatgtccatg gtacgaacga taaccgcctg cgagccacac
    76921 gcttcagcca ctgctttgta agcagcaaac tgttcttctt cagtgggcag cgcgtcgcgg
    76981 tccatgaaca ggaactcagt acgatacaga ccaacgcctt cagcgccgtt acgctctgca
    77041 ccttcaacgt cacgaaccgt accaatgtta gcgcatactt ctacttggtg accgtccagc
    77101 gtaatagctg gcagatcttt cagtttagca agctctgctt tttcagaagc cacttgctcc
    77161 tgaacagcgc gcattttatc aataacttcg ttggttggat tgacgtaaac ctgattattt
    77221 acggcatcca gaatcagata gtcgtcattt ttcacctgag aggtgacgct accggtaccc
    77281 acgatagcag gcagttccag agaacgcgcc atgatagagg tgtgggaagt acggccaccc
    77341 gcgtcggtga tgaaacccag caccttcttc aggttcagct gtgcggtttc ggacggtgtc
    77401 aggtcagcgg caaccagaat gacttcatcc tgaatggcgc tcaggtcgat aatcttcagg
    77461 cccaggatgt tgcgcagcag gcgcttaccg atatcacgta cgtcagccgc acgttctttc
    77521 aggtattcat catccagctc ttccagggca gaagcctgac cttcgataac ttcatgagca
    77581 gctgcgtcag ctgtcatgtg tttatcttta atcagggcta tgatttcctg ctccagctcc
    77641 tcatcttcga gcagcataat atgcccttca aagatggctt ctttttcttc accgaacgtt
    77701 tcaccagctt tcgttttgat cgtttccagc tgggctgatg ccttggcacg accgctcaga
    77761 aaacgttcaa cttcctgatc aacctggtcg gcagaaattt ttttccggtc aatgacaatt
    77821 tcgtcttctt tcagaagcag agctttaccg aaagcgatac ccggggatgc taaaatgcct
    77881 gaaatcataa ccctacctta cttgtgactg atttttaaaa gaacccggga aattactcga
    77941 gttccgccat cagtttaacc agatgttcaa ccgctttctg ctcgtcttcg ccttctgcgg
    78001 agatagtcac aactgtacct tgagtcaggc ccagagtctg cagtttaaac aggcttttcg
    78061 cgctggcgct tttgccgttg gaagtcacag taatttcaga agtgaagccc ttagcttctt
    78121 ttacaaactg ggcagcaggg cgggtgtgca gaccgttcgg agcggtaatg gtaacttctt
    78181 gctggaacat tgtatttccc caacttatag gtttagtgtt gtggaactaa agtctagcct
    78241 ggcggttcga ctttagcctg tattgttagc gccagttttt aacagacgcg acgcacgaag
    78301 agcgcaatac agtccattgc tggcgggtgt tgaccgctga cgtttcgtac gtcattaaac
    78361 attatgccgc taaaggaaga attgaaccaa atcataaaat cgattcagct ttggcttttc
    78421 ctgtaacgat taatttcgcg catcaaaata attagtctgg ttaaatacca gttccgacag
    78481 gctgaatcaa tgccaggagg ggcgaaagtt tgaagcaggc cacaaaaaag cacctgaaaa
    78541 ggtgcttttt tacgcatttt taacaagctg gcattactgt tgcaattctt tctcagtgaa
    78601 gagatcggca aacaatgcgg tgcttaaata acgctcaccc gatgacggta gaataaccac
    78661 aatattcttg ttgataaagc tttcatcttc ttgtagtttc aacgccgcag caacagctgc
    78721 tccagaagag atacctgcaa gaataccttc ttcttccatc agacgacgcg cggtagaaat
    78781 cgcttcttca ttggtgatgc caatgacttt atcgaccagc ttgagatcga ggttagccgg
    78841 gataaaacca gcgccaatac cctgaatttt atgcgggcca ggtttaatct cttcacctgc
    78901 cagcgcctgg gcgataactg gagaatcggt tggctcaacg gcgacagaga taagatcggt
    78961 cttacctttg gtgcctttaa tgtagcggct gacgccagtc agcgtaccgc cagtcccaac
    79021 gccagcaata aatacatcaa cctgaccgtc ggtatcttcc cagatctccg gaccggtggt
    79081 cttttcgtga atttcagggt ttgccggatt gctgaattgt tgcagcagca ggtatttctc
    79141 tggattgctg gcgacaattt cttctgcttt ttggattgcg cctttcatgc ctttagcacc
    79201 ttcggtcagc accaggtttg cacctaacgc tttcagcagc ttgcggcgtt caatactcat
    79261 ggtttctggc atggtcaggg tgagtttgta accgcgagcg gcagctacat aggccagtgc
    79321 aatcccggta ttaccgctgg ttggttcaac cagttcaacg cctggtttca gcacgccgcg
    79381 cttttcggca tcccaaatca tgttggcacc gatacggcac ttaacgctga agctggggtt
    79441 acgagattcc accttcgcca gaatgcgtcc gttaccgatg cgattcaggc gaaccagcgg
    79501 cgtgtgaccg atagtcagcg agttatcttc aaaaatctta ctcatggcct gtccttaact
    79561 gtatgaaatt gggatacaac aggtagcata cccgctcaga gaatatgcgg aagtaaggat
    79621 ttagcatatc tatatgcaga agggaaataa tgacatgcaa gatggaataa ggggcggcat
    79681 aagccccccc tgtttcacac aaacggttta caaaataacc ggtagattgt taccgccaca
    79741 tcgcgtgttt atcgcgatag caatcgaccc acatcgccgt cgcgccacaa acggcaacgg
    79801 gcataatgaa cagattaagc agcgggatca tcgtaaacag actggttaaa gcaccaaact
    79861 gcatattggt gattttgcgt gtgcgcaggg cggtgcgcat ctctttaaac ggcactttgt
    79921 ggttatcgaa ggggtaatcg caatactgga tggctaacat ccaggcgcta aacaggaacc
    79981 acagtaccgg cgcgacggtt tgcccaatac cagggatgaa gtaaagaatt agcaatacaa
    80041 ttgcgcgtgg cagataccag gcaaattttt gccattcgcg tttcatgatt cgcggcacat
    80101 ctttcatgat accgaaaatc ccggtatctg gcggtgtagc gccagtcaat cgtgcttcca
    80161 gttgttcagc caataaaccg ttaaacggag cggcaatcca gttagcaatc gtggagaaga
    80221 aatagccaaa cactaacagc acagagatga cagccagagg ccacaacaga tagctcagcc
    80281 attgtaacca gtccggaaca taactcatga gagtcgggat ccagacatcg agttgcgtaa
    80341 agagccacca gaatgcgccc cccatcaaca aaatattgac cagcagcggt aaaataacga
    80401 aacgccgaat cccaggttgc gagacgagct tccagccttg cgcaaaatag taaaaaccgc
    80461 tgcgtggggc agatgtgaat gatgaaacca taatcaggat gagctccttt tgaccaatcc
    80521 caggaaaatt gtgcgtattt taccgggtaa ttgcgcaatg gacagttagg atatgttcga
    80581 aaaaacagca aaaagcacga tttcatctat ctttgtgctg tgaaagttaa tagtgcactt
    80641 gcacttgagg taatcggcaa atactcttag tgagtaaatg tttgccgtgg tggcaaggtg
    80701 ttagaacaac agagaatata atgatgcagg atttgcgtct gatattaatc attgttggcg
    80761 cgatcgccat aatcgcttta ctggtacatg gtttctggac cagccgtaaa gaacgatctt
    80821 ctatgttccg cgatcggcca ttaaaacgaa tgaagtcaaa acgtgacgac gattcttatg
    80881 acgaggatgt cgaagatgat gagggcgttg gtgaggttcg tgttcaccgc gtgaatcatg
    80941 ccccggctaa cgcccaggag catgaggctg ctcgtccgtc gccgcaacac cagtaccaac
    81001 cgccttatgc gtctgcgcag ccgcgtcaac cggtccagca gccgcctgaa gcgcaggtac
    81061 cgccgcaaca tgctccgcgt ccagcgcagc cggtgcagca acccgtgcag cagcctgcct
    81121 atcagccgca gcctgaacag ccgttgcagc agccagtttc gccacaggtc gcgccagcgc
    81181 cgcagccagt gcattcagca ccgcaaccgg cacaacaggc tttccagcct gcagaacccg
    81241 tagcggcacc acagcctgag cctgtagcgg aaccggctcc agttatggat aaaccgaagc
    81301 gcaaagaagc ggtgattatc atgaacgtcg cggcgcatca cggtagcgag ctaaacggtg
    81361 aactgcttct taacagcatt caacaagcgg gcttcatttt tggcgatatg aatatttacc
    81421 atcgtcatct tagcccggat ggcagcggcc cggcgttatt tagcctggcg aatatggtga
    81481 aaccgggaac ctttgatcct gaaatgaagg atttcactac tccgggtgtc actatcttta
    81541 tgcaggtacc gtcttacggt gacgagctgc agaacttcaa gctgatgctg caatctgcgc
    81601 agcatattgc cgatgaagtg ggcggtgtcg tgcttgacga tcagcgccgt atgatgactc
    81661 cgcagaaatt gcgcgagtac caggacatca tccgcgaagt taaagacgcc aacgcctgat
    81721 acacttaagg caaattaact cctcttcgaa cccccgcttg tcgggggttt ttagcattga
    81781 tggtgcgata tggaatcaat cgaacaacaa ctgacagaac tgcgaacgac gcttcgccat
    81841 catgaatatc tttatcatgt gatggatgcg ccggaaattc ccgacgctga atacgacagg
    81901 ctgatgcgcg aactgcgcga gctggaaacc aaacatccag aactgattac gcctgattcg
    81961 cctactcaac gtgtaggtgc tgcgccgctg gcggctttca gccagattcg ccatgaagtg
    82021 ccaatgctgt cgctggataa cgtttttgat gaagaaagct ttcttgcctt caacaaacgt
    82081 gtgcaggacc gtctgaaaag caacgagaaa gtcacctggt gctgtgagct gaagctggat
    82141 ggccttgccg tcagtattct gtatgaaaat ggcgttttag tcagtgccgc gactcgtggc
    82201 gatggcacca ccggggaaga tatcacgtct aatgtgcgta ctattcgcgc cattccactg
    82261 aagttgcacg gagagaatat cccggcgcgt ctggaagtgc gtggtgaagt gttcctgccg
    82321 caggcggggt tcgaaaagat taacgaagat gcgcgacgca cgggcgggaa agtgtttgct
    82381 aacccacgta atgcggcagc tggttcactg cgtcagcttg atccgcgtat tacagcgaag
    82441 cgaccgctca cttttttctg ctatggcgtt ggtgttctgg aaggtggcga gctgccggat
    82501 actcatcttg gccgtttact gcaatttaaa aagtgggggt tgccggtcag cgatcgggta
    82561 acgctttgtg aatcggcgga agaagtgctg gcgttctatc acaaagtgga agaagaccgc
    82621 ccgacgctgg gctttgatat cgacggcgtg gtgattaagg tcaactcact ggaacagcag
    82681 gagcagcttg gctttgtcgc gcgagccccg cgctgggcgg tagcgtttaa attcccggcg
    82741 caggaacaga tgacctttgt gcgtgacgtc gagtttcagg ttgggcgtac tggcgcgatt
    82801 acgcctgttg cgcgtctgga acctgtccat gttgcaggcg tgctggtgag taacgcaacc
    82861 ttacacaatg cggatgaaat cgaacgtctt ggtttacgca ttggcgataa agtggtgatt
    82921 cgccgcgctg gcgacgtgat cccgcaggtg gttaacgtcg tgctttctga acgcccggaa
    82981 gatacccgtg aggttgtatt cccgacgtat tgtccggtat gtggttctga cgttgagcgt
    83041 gtggaaggtg aagcggttgc ccgctgtact ggtggtctga tttgcggtgc gcagcgtaaa
    83101 gagtcgctga aacactttgt ttcccgccgt gcgatggatg ttgacggaat gggcgacaaa
    83161 atcatcgatc agctggttga aaaagaatat gtccacactc cggcggatct gttcaaactc
    83221 accgcaggca aactgaccgg actggagcgt atggggccaa aatcggcaca aaacgtggtt
    83281 aacgcgctgg aaaaagcgaa agaaaccacc tttgctcgct tcctctatgc acttggcatc
    83341 cgtgaagtcg gcgaggccac cgcagcaggt ctggcggcat atttcggaac gctggaagcg
    83401 ctggaagccg cttcgattga agagctgcaa aaggtgcctg atgttggcat tgtcgttgca
    83461 tcccacgttc acaacttctt tgccgaagaa agcaaccgca atgtcatcag cgagctgttg
    83521 gcggaaggtg ttcactggcc tgcgccgatc gttatcaacg cggaagagat tgacagcccg
    83581 tttgctggta aaaccgtggt gcttacgggc agcttaagcc agatgtcgcg tgatgacgct
    83641 aaagctcgac tggtcgaact gggcgcgaaa gtcgcgggca gcgtgtcgaa gaaaaccgat
    83701 ctggtgatag cgggtgaagc tgcaggatct aaactggcga aggcgcagga actgggcatt
    83761 gaagtcatcg acgaagcgga aatgctgcgt ttgctgggta gctgagatgg aaaaagagca
    83821 gctgattgaa atagccaata cgataatgcc gtttggcaaa tacaaagggc gtcgcttaat
    83881 cgacctgccg gaggagtatc tgctgtggtt tgcccgcaaa gatgagttcc cggcagggaa
    83941 gctcggtgag ctaatgcaaa tcacgctgct gattaaaacc gaggggctga cgcaactggt
    84001 ccagcccctg aagcgtccgc tttaagcttt atcggcgctg ctttcctgct gcgcctgtaa
    84061 ctgttcggtc tggcgtttgt atcgacgcgc cagcaccgca cagaccatca attggatctg
    84121 atggaaaatc atcaggggca gcaccatcat accgatcacc gatgtgggga acagaatgtt
    84181 tgccatcggg atgccatttg ccagactctt tttcgaacca caaaagacga tagtaatttc
    84241 atctgcctta ttgaagccca gtcggcgtgc cataaagacg ttaactacaa tcacgatagc
    84301 cagaagaacg cagctgacca ccacgataaa cagcaatgat ccccagccaa ctttatgcca
    84361 gataccatta acgacggctt cgctgaacgc tgtataaacc accaacagaa tggacgtctg
    84421 gtcagttttc gcaatccatt ttttattgcg cgacacccag tcaccaatcc acggccggga
    84481 aagatgcccc aacacaaacg gcagcagcag ttgcagcata attttaccga cctgctcaag
    84541 gctgccccct gcaccgtgaa cattcatcac cagaccaacc agcaatggtg aaaggaaaat
    84601 ccccagcaga ctggatgctg acgcagaaca aaccgccgcc gcgacgttac cgcccgccat
    84661 tgacgtgaag gcgattgcag actgcacggt agccgggaga atgcacaagt agagaaaacc
    84721 ggagtagagc atcgggtcga catttaccgg tttccaccag gcaaacagta cacccagaat
    84781 cggaaacagc acgaaggtgc tgcacattac ccacaaatgc agtcgccagt gaccaccgcc
    84841 agcaataatc gcctcacgcg acaatttcgc gccgtgcata aagaacagca gggcaatagc
    84901 tgcggtggtc agattttcaa agaaggggac gaaatcgcct ctggcaggaa agaaagaggc
    84961 cagcaacacc accgtgatca gggttaaggt gaaaggatcg aggatacgaa aaagtttcat
    85021 aaagactcca gatgatcgat ggttctattg tgcttcttcc agtttgagaa ataaaattga
    85081 tttattgcat ccattcatga agaaaacaga tgaattattc tttaaaacaa ttaaaggttt
    85141 tcgtcacagt agcgcaggag aaaagtttta gtcgtgcagg agagcgtatc ggcctgagcc
    85201 agtcggcagt aagtcacagt gtgaaggagc tggaaaatca tactggtgtt cgcctgctgg
    85261 acagaaccac gcgtgaagtg gtgcttacag atgcagggca gcagttggct ttgcgtcttg
    85321 agcgactgct ggatgaactt aacagcacgt tgcgcgatac cgggcgtatg gggcaacaac
    85381 tgagcggaaa agttcgggtc gctgccagcc agaccatttc cgcgcatctt attccgcaat
    85441 gcattgctga aagccatcgc cgctatccag atattcagtt tgtcctgcac gatcgtccgc
    85501 agcagtgggt gatggaaagt attcgccagg gagatgtcga ctttggcatc gtcatcgatc
    85561 caggcccagt gggcgatctg caatgtgaag tgattctttc cgagcctttc tttctgcttt
    85621 gccatcggga tagcgctttg gccgcggaag attatgtacc ctggcaggca ttacagggag
    85681 cgaaattggt gttgcaggat tatgcgtcag gcagccgacc gctgattgac gcagcgctgg
    85741 cgcgcaatgg tattcaggcg aatattgtgc aggagattgg acatccggcg acgctgttcc
    85801 cgatggtagc cgcaggcatc ggcatcagta ttcttcccgc actggcgcta ccattgcctg
    85861 aaggtagccc gctggtggta aagcgcatta cgccggtggt ggaacgccag ttgatgctgg
    85921 tgcgtaggaa aaaccgttca ctttccaccg ccgctgaagc gttatgggac gtggtacgag
    85981 accagggcaa tacgctaatg gctgggcggg aaggggatcc gttgtatcag atatagatac
    86041 cgatatgagt cgtattttca acaggattta gaagggatac attcagagac aaacgctgct
    86101 ctttctcctg cgccttttct gcctgctgtc tctgcaactg cgccagttgt gtttccagta
    86161 gggtgatttg ctgttgaatc aattcggcct gtttttgctt gtcttcagca ctgccgctag
    86221 tgtctacaat ctctttaatg tgttgagtga gtttgatgat ttgctgactt atccgcgcga
    86281 tttgtgaagc aacatcgcta ccttgtgatg ccctgttact actctgtgtt tgtatggaag
    86341 gaattgaagt tgaagagata gtggccattg ttcagtcctc gtcactgtac gaggtaatat
    86401 cggcagaaga acaaggaact tgagaagaaa aatcaatctt acaaatcgca
//
LOCUS       DAZFDC010000005        86372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFDC010000005 DAZFDC010000000
VERSION     DAZFDC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.005"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            428..877
                     /gene="alaE"
                     /locus_tag="ACHJ48_000385"
     CDS             428..877
                     /gene="alaE"
                     /locus_tag="ACHJ48_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO4706019.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(914..1258)
                     /gene="ygaC"
                     /locus_tag="ACHJ48_000386"
     CDS             complement(914..1258)
                     /gene="ygaC"
                     /locus_tag="ACHJ48_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO4706020.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1410..1739
                     /gene="ygaM"
                     /locus_tag="ACHJ48_000387"
     CDS             1410..1739
                     /gene="ygaM"
                     /locus_tag="ACHJ48_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO4706021.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1889..3223)
                     /locus_tag="ACHJ48_000388"
     CDS             complement(1889..3223)
                     /locus_tag="ACHJ48_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4706022.1"
                     /translation="MPRYQDIARQLKTVIEQGKLKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLVRRGQAGTFVSEQIHPQQSAVDVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASMALILHALAQPGDGMWMEDPGFPLIRPIVTRHGVEILPVPVDDNGLDVTSGIQN
                     YPDARFALITPAHQSPLGVALSLARRYQILEWAERSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVEQISQFRQQASLEPCAVPVLWQNTL
                     ADFIREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIKLAGNDVTF
                     VHKANAAGLAVQALSDWRIRSSEDGGLLLSFTNIVSESMARQVAQQLRSALN"
     gene            3311..3742
                     /locus_tag="ACHJ48_000389"
     CDS             3311..3742
                     /locus_tag="ACHJ48_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4706023.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELIYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTDIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3951..4196
                     /gene="nrdH"
                     /locus_tag="ACHJ48_000390"
     CDS             3951..4196
                     /gene="nrdH"
                     /locus_tag="ACHJ48_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO4706024.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            4193..4603
                     /gene="nrdI"
                     /locus_tag="ACHJ48_000391"
     CDS             4193..4603
                     /gene="nrdI"
                     /locus_tag="ACHJ48_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO4706025.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4576..6720
                     /gene="nrdE"
                     /locus_tag="ACHJ48_000392"
     CDS             4576..6720
                     /gene="nrdE"
                     /locus_tag="ACHJ48_000392"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4706026.1"
                     /translation="MATTTAECLTQKTMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTGKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6730..7689
                     /gene="nrdF"
                     /locus_tag="ACHJ48_000393"
     CDS             6730..7689
                     /gene="nrdF"
                     /locus_tag="ACHJ48_000393"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4706027.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEYEDWNF"
     gene            8045..9247
                     /gene="proV"
                     /locus_tag="ACHJ48_000394"
     CDS             8045..9247
                     /gene="proV"
                     /locus_tag="ACHJ48_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO4706028.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPNELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEIINNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            9240..10304
                     /gene="proW"
                     /locus_tag="ACHJ48_000395"
     CDS             9240..10304
                     /gene="proW"
                     /locus_tag="ACHJ48_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO4706029.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10361..11353
                     /gene="proX"
                     /locus_tag="ACHJ48_000396"
     CDS             10361..11353
                     /gene="proX"
                     /locus_tag="ACHJ48_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO4706030.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11545..12729
                     /locus_tag="ACHJ48_000397"
     CDS             11545..12729
                     /locus_tag="ACHJ48_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4706031.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12853..13590
                     /gene="ygaZ"
                     /locus_tag="ACHJ48_000398"
     CDS             12853..13590
                     /gene="ygaZ"
                     /locus_tag="ACHJ48_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO4706032.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13580..13915
                     /gene="ygaH"
                     /locus_tag="ACHJ48_000399"
     CDS             13580..13915
                     /gene="ygaH"
                     /locus_tag="ACHJ48_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO4706033.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            14006..14536
                     /gene="emrR"
                     /locus_tag="ACHJ48_000400"
     CDS             14006..14536
                     /gene="emrR"
                     /locus_tag="ACHJ48_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO4706034.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14663..15835
                     /gene="emrA"
                     /locus_tag="ACHJ48_000401"
     CDS             14663..15835
                     /gene="emrA"
                     /locus_tag="ACHJ48_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO4706035.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQQMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15852..17390
                     /gene="emrB"
                     /locus_tag="ACHJ48_000402"
     CDS             15852..17390
                     /gene="emrB"
                     /locus_tag="ACHJ48_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO4706036.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17454..17969)
                     /gene="luxS"
                     /locus_tag="ACHJ48_000403"
     CDS             complement(17454..17969)
                     /gene="luxS"
                     /locus_tag="ACHJ48_000403"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO4706037.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     VWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(18119..19675)
                     /gene="gshA"
                     /locus_tag="ACHJ48_000404"
     CDS             complement(18119..19675)
                     /gene="gshA"
                     /locus_tag="ACHJ48_000404"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO4706038.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIDKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(19748..20176)
                     /gene="yqaA"
                     /locus_tag="ACHJ48_000405"
     CDS             complement(19748..20176)
                     /gene="yqaA"
                     /locus_tag="ACHJ48_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO4706039.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFCPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(20173..20739)
                     /gene="yqaB"
                     /locus_tag="ACHJ48_000406"
     CDS             complement(20173..20739)
                     /gene="yqaB"
                     /locus_tag="ACHJ48_000406"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO4706040.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(21020..21096)
                     /locus_tag="ACHJ48_000407"
     tRNA            complement(21020..21096)
                     /locus_tag="ACHJ48_000407"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21060..21062),aa:Arg,seq:acg)
     gene            complement(21199..21275)
                     /locus_tag="ACHJ48_000408"
     tRNA            complement(21199..21275)
                     /locus_tag="ACHJ48_000408"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21239..21241),aa:Arg,seq:acg)
     gene            complement(21338..21414)
                     /locus_tag="ACHJ48_000409"
     tRNA            complement(21338..21414)
                     /locus_tag="ACHJ48_000409"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21378..21380),aa:Arg,seq:acg)
     gene            complement(21479..21555)
                     /locus_tag="ACHJ48_000410"
     tRNA            complement(21479..21555)
                     /locus_tag="ACHJ48_000410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21519..21521),aa:Arg,seq:acg)
     gene            complement(21559..21651)
                     /locus_tag="ACHJ48_000411"
     tRNA            complement(21559..21651)
                     /locus_tag="ACHJ48_000411"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21615..21617),aa:Ser,seq:gct)
     gene            complement(21967..22152)
                     /gene="csrA"
                     /locus_tag="ACHJ48_000412"
     CDS             complement(21967..22152)
                     /gene="csrA"
                     /locus_tag="ACHJ48_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO4706041.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(22387..25017)
                     /gene="alaS"
                     /locus_tag="ACHJ48_000413"
     CDS             complement(22387..25017)
                     /gene="alaS"
                     /locus_tag="ACHJ48_000413"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO4706042.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(25145..25645)
                     /gene="recX"
                     /locus_tag="ACHJ48_000414"
     CDS             complement(25145..25645)
                     /gene="recX"
                     /locus_tag="ACHJ48_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO4706043.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25713..26774)
                     /gene="recA"
                     /locus_tag="ACHJ48_000415"
     CDS             complement(25713..26774)
                     /gene="recA"
                     /locus_tag="ACHJ48_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO4706044.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26854..27351)
                     /gene="pncC"
                     /locus_tag="ACHJ48_000416"
     CDS             complement(26854..27351)
                     /gene="pncC"
                     /locus_tag="ACHJ48_000416"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO4706045.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITLRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27496..28581)
                     /gene="mltB"
                     /locus_tag="ACHJ48_000417"
     CDS             complement(27496..28581)
                     /gene="mltB"
                     /locus_tag="ACHJ48_000417"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO4706046.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28836..29398
                     /gene="srlA"
                     /locus_tag="ACHJ48_000418"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28836..29398
                     /gene="srlA"
                     /locus_tag="ACHJ48_000418"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            29395..30356
                     /gene="srlE"
                     /locus_tag="ACHJ48_000419"
                     /gene_synonym="gutE"
                     /pseudo
     CDS             29395..30356
                     /gene="srlE"
                     /locus_tag="ACHJ48_000419"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
     gene            30367..30738
                     /gene="srlB"
                     /locus_tag="ACHJ48_000420"
                     /gene_synonym="gutB"
     CDS             30367..30738
                     /gene="srlB"
                     /locus_tag="ACHJ48_000420"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO4706047.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30742..31521
                     /gene="srlD"
                     /locus_tag="ACHJ48_000421"
     CDS             30742..31521
                     /gene="srlD"
                     /locus_tag="ACHJ48_000421"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO4706048.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFVRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31627..31986
                     /gene="gutM"
                     /locus_tag="ACHJ48_000422"
     CDS             31627..31986
                     /gene="gutM"
                     /locus_tag="ACHJ48_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO4706049.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            32053..32826
                     /gene="srlR"
                     /locus_tag="ACHJ48_000423"
                     /gene_synonym="gutR"
     CDS             32053..32826
                     /gene="srlR"
                     /locus_tag="ACHJ48_000423"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO4706050.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            32819..33784
                     /gene="gutQ"
                     /locus_tag="ACHJ48_000424"
     CDS             32819..33784
                     /gene="gutQ"
                     /locus_tag="ACHJ48_000424"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO4706051.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33781..35295)
                     /gene="norR"
                     /locus_tag="ACHJ48_000425"
     CDS             complement(33781..35295)
                     /gene="norR"
                     /locus_tag="ACHJ48_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO4706052.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35482..36921
                     /gene="norV"
                     /locus_tag="ACHJ48_000426"
     CDS             35482..36921
                     /gene="norV"
                     /locus_tag="ACHJ48_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO4706053.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36918..38051
                     /gene="norW"
                     /locus_tag="ACHJ48_000427"
     CDS             36918..38051
                     /gene="norW"
                     /locus_tag="ACHJ48_000427"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO4706054.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(38179..40431)
                     /gene="hypF"
                     /locus_tag="ACHJ48_000428"
     CDS             complement(38179..40431)
                     /gene="hypF"
                     /locus_tag="ACHJ48_000428"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO4706055.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPYLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFSLVRGEQVVLSQHLGDLSDDGIQTQWREALRLMQNIYNFTPQYVV
                     HDAHPGYVSCQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(40584..41111)
                     /gene="hydN"
                     /locus_tag="ACHJ48_000429"
     CDS             complement(40584..41111)
                     /gene="hydN"
                     /locus_tag="ACHJ48_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO4706056.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(41260..42270)
                     /gene="ascG"
                     /locus_tag="ACHJ48_000430"
     CDS             complement(41260..42270)
                     /gene="ascG"
                     /locus_tag="ACHJ48_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO4706057.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTSTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            42530..43987
                     /gene="ascF"
                     /locus_tag="ACHJ48_000431"
     CDS             42530..43987
                     /gene="ascF"
                     /locus_tag="ACHJ48_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO4706058.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            43996..45420
                     /gene="ascB"
                     /locus_tag="ACHJ48_000432"
     CDS             43996..45420
                     /gene="ascB"
                     /locus_tag="ACHJ48_000432"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO4706059.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNTNNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(45544..46014)
                     /gene="hycI"
                     /locus_tag="ACHJ48_000433"
     CDS             complement(45544..46014)
                     /gene="hycI"
                     /locus_tag="ACHJ48_000433"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO4706060.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(46007..46417)
                     /gene="hycH"
                     /locus_tag="ACHJ48_000434"
     CDS             complement(46007..46417)
                     /gene="hycH"
                     /locus_tag="ACHJ48_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO4706061.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(46414..47181)
                     /gene="hycG"
                     /locus_tag="ACHJ48_000435"
     CDS             complement(46414..47181)
                     /gene="hycG"
                     /locus_tag="ACHJ48_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO4706062.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(47181..47723)
                     /gene="hycF"
                     /locus_tag="ACHJ48_000436"
     CDS             complement(47181..47723)
                     /gene="hycF"
                     /locus_tag="ACHJ48_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO4706063.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(47733..49442)
                     /gene="hycE"
                     /locus_tag="ACHJ48_000437"
     CDS             complement(47733..49442)
                     /gene="hycE"
                     /locus_tag="ACHJ48_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO4706064.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(49460..50383)
                     /gene="hycD"
                     /locus_tag="ACHJ48_000438"
     CDS             complement(49460..50383)
                     /gene="hycD"
                     /locus_tag="ACHJ48_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO4706065.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVASSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(50386..52212)
                     /gene="hycC"
                     /locus_tag="ACHJ48_000439"
     CDS             complement(50386..52212)
                     /gene="hycC"
                     /locus_tag="ACHJ48_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO4706066.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(52209..52820)
                     /gene="hycB"
                     /locus_tag="ACHJ48_000440"
     CDS             complement(52209..52820)
                     /gene="hycB"
                     /locus_tag="ACHJ48_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO4706067.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(52945..53406)
                     /gene="hycA"
                     /locus_tag="ACHJ48_000441"
     CDS             complement(52945..53406)
                     /gene="hycA"
                     /locus_tag="ACHJ48_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO4706068.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            53618..53968
                     /gene="hypA"
                     /locus_tag="ACHJ48_000442"
     CDS             53618..53968
                     /gene="hypA"
                     /locus_tag="ACHJ48_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4706069.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            53972..54844
                     /gene="hypB"
                     /locus_tag="ACHJ48_000443"
     CDS             53972..54844
                     /gene="hypB"
                     /locus_tag="ACHJ48_000443"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO4706070.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAC
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            54835..55107
                     /gene="hypC"
                     /locus_tag="ACHJ48_000444"
     CDS             54835..55107
                     /gene="hypC"
                     /locus_tag="ACHJ48_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO4706071.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            55107..56228
                     /gene="hypD"
                     /locus_tag="ACHJ48_000445"
     CDS             55107..56228
                     /gene="hypD"
                     /locus_tag="ACHJ48_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO4706072.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPAVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            56225..57235
                     /gene="hypE"
                     /locus_tag="ACHJ48_000446"
     CDS             56225..57235
                     /gene="hypE"
                     /locus_tag="ACHJ48_000446"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO4706073.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPTNIHWG
                     AQTLTAGDILLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            57309..59387
                     /gene="flhA"
                     /locus_tag="ACHJ48_000447"
     CDS             57309..59387
                     /gene="flhA"
                     /locus_tag="ACHJ48_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO4706074.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(59424..59777)
                     /gene="ygbA"
                     /locus_tag="ACHJ48_000448"
     CDS             complement(59424..59777)
                     /gene="ygbA"
                     /locus_tag="ACHJ48_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO4706075.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKCP
                     VPELPEKYRPKKPRE"
     gene            complement(59854..59988)
                     /locus_tag="ACHJ48_000449"
     CDS             complement(59854..59988)
                     /locus_tag="ACHJ48_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706076.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     "
     gene            60064..62625
                     /gene="mutS"
                     /locus_tag="ACHJ48_000450"
     CDS             60064..62625
                     /gene="mutS"
                     /locus_tag="ACHJ48_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO4706077.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            62731..63387
                     /gene="pphB"
                     /locus_tag="ACHJ48_000451"
     CDS             62731..63387
                     /gene="pphB"
                     /locus_tag="ACHJ48_000451"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4706078.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(63428..63664)
                     /locus_tag="ACHJ48_000452"
     CDS             complement(63428..63664)
                     /locus_tag="ACHJ48_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO4706079.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(63675..65102)
                     /locus_tag="ACHJ48_000453"
     CDS             complement(63675..65102)
                     /locus_tag="ACHJ48_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO4706080.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(65102..65695)
                     /locus_tag="ACHJ48_000454"
     CDS             complement(65102..65695)
                     /locus_tag="ACHJ48_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO4706081.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPYARRWQGLPQARNFSQENE"
     gene            65842..66249
                     /locus_tag="ACHJ48_000455"
     CDS             65842..66249
                     /locus_tag="ACHJ48_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO4706082.1"
                     /translation="MTLRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(66369..67361)
                     /gene="rpoS"
                     /locus_tag="ACHJ48_000456"
     CDS             complement(66369..67361)
                     /gene="rpoS"
                     /locus_tag="ACHJ48_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO4706083.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(67424..68518)
                     /gene="nlpD"
                     /locus_tag="ACHJ48_000457"
     CDS             complement(67424..68518)
                     /gene="nlpD"
                     /locus_tag="ACHJ48_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO4706084.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPAHTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(68703..69329)
                     /gene="pcm"
                     /locus_tag="ACHJ48_000458"
     CDS             complement(68703..69329)
                     /gene="pcm"
                     /locus_tag="ACHJ48_000458"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO4706085.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(69323..70084)
                     /gene="surE"
                     /locus_tag="ACHJ48_000459"
     CDS             complement(69323..70084)
                     /gene="surE"
                     /locus_tag="ACHJ48_000459"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO4706086.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(70065..71114)
                     /gene="truD"
                     /locus_tag="ACHJ48_000460"
     CDS             complement(70065..71114)
                     /gene="truD"
                     /locus_tag="ACHJ48_000460"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO4706087.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELVELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(71111..71590)
                     /gene="ispF"
                     /locus_tag="ACHJ48_000461"
     CDS             complement(71111..71590)
                     /gene="ispF"
                     /locus_tag="ACHJ48_000461"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO4706088.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(71590..72300)
                     /gene="ispD"
                     /locus_tag="ACHJ48_000462"
     CDS             complement(71590..72300)
                     /gene="ispD"
                     /locus_tag="ACHJ48_000462"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO4706089.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(72319..72630)
                     /gene="ftsB"
                     /locus_tag="ACHJ48_000463"
     CDS             complement(72319..72630)
                     /gene="ftsB"
                     /locus_tag="ACHJ48_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO4706090.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(72824..73147)
                     /gene="ygbE"
                     /locus_tag="ACHJ48_000464"
     CDS             complement(72824..73147)
                     /gene="ygbE"
                     /locus_tag="ACHJ48_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO4706091.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLMTVGIMFGALF
                     MWLLG"
     gene            complement(73197..73802)
                     /gene="cysC"
                     /locus_tag="ACHJ48_000465"
     CDS             complement(73197..73802)
                     /gene="cysC"
                     /locus_tag="ACHJ48_000465"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO4706092.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(73802..75229)
                     /gene="cysN"
                     /locus_tag="ACHJ48_000466"
     CDS             complement(73802..75229)
                     /gene="cysN"
                     /locus_tag="ACHJ48_000466"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO4706093.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRSRVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGEK"
     gene            complement(75231..76139)
                     /gene="cysD"
                     /locus_tag="ACHJ48_000467"
     CDS             complement(75231..76139)
                     /gene="cysD"
                     /locus_tag="ACHJ48_000467"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO4706094.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHQNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            76391..77428
                     /gene="iap"
                     /locus_tag="ACHJ48_000468"
     CDS             76391..77428
                     /gene="iap"
                     /locus_tag="ACHJ48_000468"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO4706095.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPQSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   77510..77722
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=77571..77600
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacaca"
     gene            complement(77818..78111)
                     /gene="cas2e"
                     /locus_tag="ACHJ48_000469"
     CDS             complement(77818..78111)
                     /gene="cas2e"
                     /locus_tag="ACHJ48_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO4706096.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(78108..79031)
                     /gene="cas1e"
                     /locus_tag="ACHJ48_000470"
     CDS             complement(78108..79031)
                     /gene="cas1e"
                     /locus_tag="ACHJ48_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO4706097.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEKVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(79028..79678)
                     /gene="cas6e"
                     /locus_tag="ACHJ48_000471"
     CDS             complement(79028..79678)
                     /gene="cas6e"
                     /locus_tag="ACHJ48_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO4706098.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEV"
     gene            complement(79660..80406)
                     /gene="cas5e"
                     /locus_tag="ACHJ48_000472"
     CDS             complement(79660..80406)
                     /gene="cas5e"
                     /locus_tag="ACHJ48_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO4706099.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(80417..81472)
                     /gene="cas7e"
                     /locus_tag="ACHJ48_000473"
     CDS             complement(80417..81472)
                     /gene="cas7e"
                     /locus_tag="ACHJ48_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO4706100.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(81484..82020)
                     /gene="casB"
                     /locus_tag="ACHJ48_000474"
                     /gene_synonym="cse2"
     CDS             complement(81484..82020)
                     /gene="casB"
                     /locus_tag="ACHJ48_000474"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO4706101.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAALSANVKAIDERLPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRVVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRHQRPTEFIRI
                     RWALEYYQAGDVDNEQNQ"
     gene            complement(82017..83579)
                     /gene="casA"
                     /locus_tag="ACHJ48_000475"
                     /gene_synonym="cse1"
     CDS             complement(82017..83579)
                     /gene="casA"
                     /locus_tag="ACHJ48_000475"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO4706102.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHPFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(83677..>86372)
                     /gene="cas3"
                     /locus_tag="ACHJ48_000476"
     CDS             complement(83677..>86372)
                     /gene="cas3"
                     /locus_tag="ACHJ48_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO4706103.1"
                     /translation="KYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDGYHLLCWHSLDVA
                     AMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLAPEL
                     KIPEGSRKNYEKISHSTLGYWLWNYYLSECEELLPSSSLSSRKLTRVIEMWMSITTGH
                     HGRPPDRIDELDNFLPEDKAAARDFLLEIKALFPLIEIPTFWDDDEGVELLKQLSWYI
                     SATVVLADWTGSSTRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGINT
                     LFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQGL
                     FFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGS
                     EEPDEQTFSQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKIL
                     LADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQE
                     APFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQGK
                     CIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKEDG
                     SQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGK
                     DERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPH
                     AARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLP
                     EKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEG
                     EAFRQWCIEQRQDPEMANVILVTDDESCGYSAREGLIGKVD"
BASE COUNT        20485 a        23460 c        22071 g        20356 t
ORIGIN      
        1 aaaccaaagt gaataaaaat gcaatataac agaataaaat aaaacacccc tcactggtgc
       61 tttctgctgt taatgtacat tttctggcat aaaaaatgct gtaaatgacc cggtcacgac
      121 atacaccgaa ttctctacgc ttatagcgtt acctcacccc caaactttca ttagtgaaat
      181 taaaggaaag gtcatatcca attgatttaa ttgaaaataa atcatatcgg catgtaaaaa
      241 tccacaatgc acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctgactaaat
      301 aaaaattttt cactaattga ttagtcatag ccagcgatat acgctatgcg aaaatgcaga
      361 tggcaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
      421 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
      481 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
      541 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
      601 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
      661 gataaaaaat ctggcggata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
      721 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
      781 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
      841 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
      901 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
      961 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     1021 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgtcg
     1081 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     1141 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     1201 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     1261 ttgatcctcg ttattatcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     1321 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     1381 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     1441 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     1501 tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     1561 aggcattgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     1621 cgcgcgatgc cgtaggctgc gcggattctt ttgttcgtga aagaccctgg tgtagtgtgg
     1681 gaactgctgc tgcggtaggt atttttatcg gtgcgttgtt gagcatgcgt aaatcgtagt
     1741 gagacgatga caagcagtag tctgacttct gaaaagtact cagtgcgtca acgccttgtc
     1801 cgcctacagg cctgaaaaac atagcgcatc aggggatttt gccctggtca ccagtcttat
     1861 aggaccccga agcctggctt cggggttatt aatttaaagc actgcgcaat tgctgtgcta
     1921 cctgtcgcgc catactttcg ctaacgatat tcgtaaacga gagcaataat cctccgtcct
     1981 cgcttgagcg gatacgccag tcgctaagtg cctgtacggc aagaccagca gcattggctt
     2041 tatgcacgaa tgtcacgtca ttacctgcta gtttgatcac catctggata ccgccttttt
     2101 gcggcacaac ctgaaatccc tgctgcgtaa gtgcctgctc aatccactgc cgtcgctggg
     2161 cataatgctg gcgcattttc ttcagatgcc gccagaaatg cccctcacgg atgaaatctg
     2221 ccagtgtgtt ctgccacagt acgggtacag cacatggttc cagtgacgcc tgctgccgga
     2281 attgcgaaat ctgctccacc ggcaccacca gccacgcaca gcgcaatgca ggaaatagcg
     2341 ctttgctgaa tgttccggca taaatcaccc gctgcggcgc gtcgagactt tttagtgccg
     2401 gtaacggctt accgtgatag cgaaactcac tgtcgtaatc atcttcaata atccatgcct
     2461 gactacgctc tgcccattcc agtatctgat acctacgcgc taaagagagt gccacaccca
     2521 gcgggctttg gtgtgccgga gtaatcaggg caaaacgcgc atcaggataa ttttgtattc
     2581 cgcttgtgac atccagtccg ttgtcatcaa ccggcacagg taaaatttcc acaccgtggc
     2641 gagtgacaat cgggcgaatc agcggaaagc cgggatcttc catccacatc ccgtctcccg
     2701 gttgcgccag agcgtgcaga attaaagcca ttgaggccgc ataaccgtga gtgataaaga
     2761 cctgctctgg ctgacaatca atcccacgtg aaacgcgtaa ataatcaaca atagcctcgc
     2821 gcaaggccgc ctcgccgcag acatcgccta atgccaaatc aaagcgcgtc tgggtacgca
     2881 gacggcggcc catcacccgc gcccacaatt cacgcggaaa aagatcaagt gcaggtagcc
     2941 ccatctgaaa aggcagcggc tgctgacttt caccagcaaa agccacgaca tccacagcag
     3001 actgttgcgg atgtatctgc tcactgacaa aggtacccgc ctgtccccgc ctgaccagcc
     3061 atccctgcgc caccagctca gcataagcat tttcgacagt ggatcgagag acacccagct
     3121 cctgcgacca ggtacggctc gaaggtagcc ttgcgccagg tttcagtttt ccttgctcga
     3181 taaccgtttt taactgacgg gcgatatcct gataacgtgg catatggtct gcttttttag
     3241 gtaatttatg gctctcttta gcagtccatt atagtgctac attgcgggcg ttaataagag
     3301 gagaaactgt atgactacat tacgtcagcc ttactacgaa cttagcccgg cagtgtataa
     3361 cgcactggtg caggccaaaa cggcactgga aaatagcacg ctggatacca cgttgatgga
     3421 gttaatttat ttgcgcgtct cgcaaatcaa cggctgcgca ttttgtctgg agatgcacag
     3481 caaagcattg cgcaaatccg gtgtgccaca acacaaactg gacgccctgg caggctggcg
     3541 cgtaagccat cattttgatg aacgcgagcg agcggcgctg gcatgggcgg aatctgtgac
     3601 cgatattgcc agaacccatg cggaagacga ggtttatcag cctttgcttg agcatttcag
     3661 cgcagcagaa atcagcgact taacgtttgc catcgggctg atgaattgtt ttaaccgtct
     3721 ggccgtttcc atgcggatgt aatttgctgt tgccgggtat gcgcccggca aaaaaacaca
     3781 aaatccccta ccccgttgag ccattcaccg gtggcgtaat caatctaaaa ttaccatatc
     3841 ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca gagagacaac
     3901 ccgacgcgta tcatcgcgcc gtatcttcat tttaaacgga aatacgaatc atgcgcatta
     3961 ctatttacac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg atggaaaacc
     4021 ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcggca gaagcgttgc
     4081 gtgctcaggg ctttcgtcag ttgccggtag tgattgctgg cgatcttagc tggtctggtt
     4141 tccgtccgga catgattaac cgtctgcatc cagcgccaca cgcggccagt gcatgagcca
     4201 gctcgtctac ttctccagca gctccgaaaa cacgcagcgt tttatcgaac gtttaggtct
     4261 gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg agccttacat
     4321 cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac gacaggtaat
     4381 tcgcttttta aacgacgagc acaaccgggc gttgcttcgc ggcgttattg cttcgggtaa
     4441 tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga aatgcggcgt
     4501 gccgtggctg taccgttttg aactcatggg tacgcaaagc gatatcgaaa acgttcgtaa
     4561 aggagtaacc gaattttggc aacgacaacc gcagaatgcc tgacgcagaa aacgatggat
     4621 taccacgcgc tgaatgcgat gcttaacctc tacgatagcg caggtcgcat tcagttcgat
     4681 aaagaccgcc aggccgttga cgcctttatg gcgacgcatg tgcgtccgaa cagtgtgacc
     4741 ttcagtagcc agcagcagcg cctgaactgg ctggtcaacg aaggttacta tgatgaaagc
     4801 gttcttaatc gctactctcg cgactttgtc attacgctgt ttgcccacgc acacaccagc
     4861 ggttttcgtt tccagacatt cctcggggca tggaagtttt acaccagcta tacgttgaag
     4921 acattcgacg gtaaacgtta tctggaagat tttgccgatc gagtcacgat ggtggcgctg
     4981 acgctggcac aaggcgatga gacgctggcg ttgcaactga cagatgaaat gctgtcagga
     5041 cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg cggcgaactg
     5101 gtttcctgtt ttttgctgcg tattgaagac aatatggagt cgattggtcg ggcggtaaat
     5161 tccgcactgc aactgtcgaa acgcggcggc ggcgtagcat ttttgctgtc gaatctgcgg
     5221 gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtaat tccggtgatg
     5281 aaaatgctgg aagacgcatt ttcctatgcc aaccaactcg gcgctcgtca gggggctggt
     5341 gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgacac gaaacgggaa
     5401 aatgccgacg aaaaaatccg cattaaaaca ctgtcgcttg gcgtggtgat cccggatatc
     5461 actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcctta tgacgtagag
     5521 cgagtttatg gcaagccgtt tgccgatgtg gccatcagcc aacactatga cgaactggtt
     5581 gccgatgaac gcattcgcaa aaaatacctc aacgcccgtg atttcttcca gcgactggca
     5641 gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacggt aaaccgtgct
     5701 aaccctgtcg ccgggcgcat aaatatgagt aatctctgct cagaaatttt gcaggttaac
     5761 agcgcctcag agtatgacga gaatctcgac tatgcccgca caggccatga tatctcctgc
     5821 aatttaggtt cgttgaatat tgcgcacacc atggattccc ccgattttgc ccgcacggta
     5881 gagactgccg tgcgcggttt aacggcagta tcagatatga gtcatatccg cagcgtgccg
     5941 tccatcgaag ccggaaatgc cgcctcgcac gccatcggac tggggcagat gaatttacac
     6001 ggctatctgg cgcgagaagg catcgcttat ggttcgccgg aagcactgga tttcaccaat
     6061 ctctatttct atgccatcac ctggcacgca ctgcgtacct cgatgttgct ggcacgcgaa
     6121 cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga atattttagc
     6181 caatatctgc aagggaactg gcagccgaaa acggggaaag ttggcgaact gtttgcccgt
     6241 agcggtatta cgttacctac ccgtgagatg tgggcgcaac tgcgcgacga cgtgatgcgc
     6301 tacggcattt acaaccagaa tcttcaggcg gtgccgccaa caggttctat ctcttatatc
     6361 aaccatgcta cgtcgagtat tcatccgatt gtggcgaaag tagagatacg caaagagggc
     6421 aaaacaggac gcgtttacta ccctgccccg tttatgacta acgagaatct ggcgctgtat
     6481 caggacgctt acgaaattgg cgcagaaaag atcatcgaca cctacgcgga agcgactcgc
     6541 catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac cactcgcgat
     6601 atcaacaaag cgcagattta cgcctggcgc aaaggtatca aaacgctcta ttacatccgg
     6661 ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg tgcactttaa
     6721 ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat atctgacgat
     6781 aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga aaaggtgccg
     6841 ctgtcgaacg atattcctgc ctggcagaca ttaactgtcg tagaacaaca actgacgatg
     6901 cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg cgcgccttcg
     6961 ctgatgcccg atgcactcac gcctcatgaa gaagcggtat tatcgaatat cagctttatg
     7021 gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca gaccaaagat
     7081 gtcgatgccg cctacgcctg gagtgaagaa aacgcgccgt tgcagcgaaa agcacagatt
     7141 attcagcaac attaccgcgg tgatgatccg ctgaaaaaga aaatcgccag tgtgtttctt
     7201 gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag ccgcggaaag
     7261 ctgaccaata ccgcggacct gatccgtctg attatccgcg atgaagcagt ccacggttac
     7321 tacataggct ataaatatca gaaaaacatg gaaaagatat ctctgggaca acgtgaagag
     7381 ttgaagagtt tcgccttcga tttgttgctg gaactctacg acaacgagtt gcaatacacc
     7441 gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct ctgttacaac
     7501 gccaataagg ctttgatgaa tctgggctac gaaccgttat ttcccgcaga aatggcggaa
     7561 gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca cgatttcttt
     7621 tccggttcag gctcctctta tgtgatgggg aaagcggttg aaacagaata tgaagactgg
     7681 aatttctgag ggtgttattt tcaaaaatat cactacccgc gacagggaaa taattcccgc
     7741 caaatagctt tttatcacgc aaatattttg tggtgatcta cactgatact ctgttgcatt
     7801 attcgcctga aaccacaata ttcaggcgtt ttttcgctat ctttgacaaa aaatatcaac
     7861 ttttctcgat ttgctctcag cccttatatc acgggaaatt ccggcgattt gctcgcatca
     7921 atattcatgc cacatttgcc atcaggggtt gcctcagatt ctcagtatgt tagggtagaa
     7981 aaaagtgact atttccattg ggtaatatat cgacataaac aaataacagg aatctttcta
     8041 ttgcatggca attaaattag aaattaaaaa tctttataaa atatttggtg agcatccaca
     8101 gcgagcgttc aaatatatcg aacaaggact ttcaaaagaa caaattctgg aaaaaactgg
     8161 gctatcgctt ggcgtaaaag acgccagtct ggccattgaa gaaggcgaga tatttgtcat
     8221 catgggatta tccggctcgg gtaaatccac aatggtacgc cttctcaatc gcctgattga
     8281 acccacccgc gggcaagtac tgattgatgg tgtggatatt gccaaaatat ccgacgccga
     8341 actccgtgag gtgcgcagaa aaaagattgc gatggtcttc cagtcctttg ccttaatgcc
     8401 gcatatgacc gtgctggaca atactgcgtt tggtatggaa ttggccggaa ttaatgccga
     8461 agaacgccgg gaaaaagccc ttgatgcact gcgtcaggtc gggctggaaa attatgccca
     8521 cagctacccg aatgaactct ctggcgggat gcgtcaacgt gtgggattag cccgcgcgtt
     8581 agcgattaat ccggatattt tattaatgga tgaagccttc tcggcgctcg atccattaat
     8641 tcgcaccgag atgcaggatg agctggtaaa attacaggcg aaacatcagc gcaccattgt
     8701 ctttatttcc cacgatctcg atgaagccat gcgtattggc gaccgaattg ccattatgca
     8761 aaatggtgaa gtggtacagg tcggcacacc ggatgaaatt atcaataatc cggcgaatga
     8821 ttatgtccgt accttcttcc gtggcgttga tattagtcag gtattcagtg cgaaagatat
     8881 tgcccgccgg acaccgaatg gcttaattcg taaaacccct ggcttcggcc cacgttcggc
     8941 actgaaatta ttgcaggatg aagatcgtga atatggctac gttatcgaac gcggtaataa
     9001 gtttgtcggc gcagtctcca tcgattcgct taaaaccgcg ttaacgcagc agcaaggtct
     9061 tgatgcggcg ctgattgatg cgccgttagc agtcgatgca caaacgcctc ttagcgagtt
     9121 gctctctcat gtcggacagg ctccctgtgc ggtgcccgtg gtcgacgagg accaacagta
     9181 tgtcggcatc atttcgaaag gaatgctgct gcgcgcttta gatcgtgagg gggtaaataa
     9241 tggctgatca aaataatccg tgggatacca cgccagcggc ggacagtgcc gcgcaatccg
     9301 cagacgcctg gggtacaccg gcgactgcac cgactgacgg cggtggtgct gactggctga
     9361 ccagtacgcc tgcgccaaac gtcgagcatt ttaatattct cgatccgttc cataaaacgc
     9421 tgatcccgct cgacagttgg gtcactgaag ggatcgactg ggtcgttacc catttccgtc
     9481 ccgtcttcca gggcgtgcgc gttccggttg attatatcct caacggtttc cagcaattgc
     9541 tgctgggtat gcccgcaccg gtggcgatta tcgttttcgc tctcatcgcc tggcagattt
     9601 ccggggtcgg aatgggcgtg gcgacgctgg tttcgctgat tgccatcggc gcaatcggtg
     9661 cctggtcgca ggcaatggtt actctggcgc tggtgttaac cgccctgctg ttctgtatcg
     9721 tcatcggttt gccgttgggg atctggctgg cgagaagtcc gcgagcggcg aaaattattc
     9781 gtccactgct tgatgccatg cagaccacgc cagcgtttgt ttatctggtg ccaatcgtca
     9841 tgctatttgg tatcggtaac gtgccgggcg tggtggtgac gatcatcttt gctctgccgc
     9901 cgattatccg tctgaccatt ctggggatta accaggttcc ggcggatctg attgaagcct
     9961 cgcgctcatt cggtgccagc ccgcgccaga tgctgttcaa agttcagtta ccgctggcga
    10021 tgccgaccat tatggcgggc gttaaccaga cgctgatgct ggccctttct atggtggtca
    10081 tcgcctcgat gattgccgtc ggcgggttgg gtcagatggt acttcgtggt atcggtcgtc
    10141 tggatatggg gcttgccacc gttggcggcg tcgggattgt gatcctcgcc attatcctcg
    10201 atcgtctgac gcaggccgtt gggcgcgact cacgcagtcg cggcaaccgt cgctggtaca
    10261 ccactggccc tgtcggtctg ctgacccgcc cattcattaa gtaactctgc acttgcccgg
    10321 tgacgccggg catatcaccc tgccaaaaaa aggaataaca atgcgacata gcgtactttt
    10381 tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgacc tgccgggcaa
    10441 aggcattact gttaatccgg ttcagagcac catcaccgaa gaaactttcc agacgctgct
    10501 ggtcagccgc gcgctggaga aattaggtta taccgtcaat aaacccagcg aagtggatta
    10561 caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac
    10621 gccactgcat gacaacatgt acgaagctgc cggtggcgat aagaaatttt atcgtgaagg
    10681 ggtatttgtt aacggcgcgg cacagggtta cctgatcgat aagaaaaccg ccgaccagta
    10741 caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt tcgataccaa
    10801 cggcgacgga aaagcggatt taaccggttg taaccctggc tggggctgcg aaggtgcgat
    10861 caaccaccag cttgccgcgt atgaactgac caataccgtg acgcataatc aggggaacta
    10921 cgcggcgatg atggccgaca ccatcagtcg ttacaaagag ggcaaaccgg tgttttacta
    10981 cacctggacg ccgtactggg tgagtaatga gctgaagcca ggcaaagatg tggtctggtt
    11041 gcaggtgccg ttctctgcac tgccgggcga taaaaatgcc gataccaaac tgccgaatgg
    11101 cgcgaattac ggcttcccgg tcagcaccat gcatatcgtt gccaataaag cctgggccga
    11161 gaaaaacccg gcagcagcga aactgtttgc cattatgcag ttgccagtgg cagatattaa
    11221 cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc agggccacgt
    11281 tgatggctgg atcaaagccc accagcaaca gttcgatggc tgggtgaatg aggcgttggc
    11341 agcgcagaag taatttttat tcgggcggat aaggcgttta cgccgcatcc gccgttctgt
    11401 acacaatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaccac accgcacatt
    11461 tcaacaatct atctttcatc ccatattcat caacatccgc tattattgat ttccagctta
    11521 atcatcacct gatgaacaaa aataatgacc aaacctaatc atgagcttag cccggcgcta
    11581 atcgtgctga tgtctatcgc caccggtctg gcggtcgcca gcaactatta cgcccagcca
    11641 ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg ctttattgtt
    11701 accgccgcgc agttgggcta tgccgccggt ctgctgtttc ttgttcccct cggtgatatg
    11761 tttgaacgcc gccgcctgat tgtctcgatg accttactgg cagcgggcgg tatgttaatc
    11821 actgccagca gtcagtcact ggcgatgatg atcctcggta cggcattaac cggtttattc
    11881 tcagtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc ggataaacgc
    11941 ggcaaagtgg ttggcaccat tatgagcggt ctgctgttgg ggatattgct ggcacgaacc
    12001 gttgccgggt tactggcaaa cctcggcggc tggcgcaccg tcttttgggt tgcctcggtg
    12061 ttaatggctc tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa atcagaaacc
    12121 cacctcaact acccacagtt gttgggttcc gttttcagca tgtttatcag caataagatc
    12181 ctgcgcaccc gcgcgttgct gggctgcctg acctttgcca acttcagcat tctctggacc
    12241 tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtgt aattggtctg
    12301 tttgggcttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg ttttgccgac
    12361 aagggcaaat cgcaccacac cacaactttc ggtctgcttc tgctgttact ttcatggctg
    12421 gcaatctggt ttggtcacac ttccgtactg gcgttgatta tcggcattct ggtgctggac
    12481 ctcaccgtac agggcgtgca tatcactaac cagacggtaa tttatcgaat acatcctgat
    12541 gcgcgtaatc gcctgaccgc aggttacatg accagctact ttattggcgg tgccgccggt
    12601 tcgctaattt cagcctcagc ctggcaacat ggcggttggg ctggcgtttg tctggctggc
    12661 gcgacgattg ccctggttaa cttactggtc tggtggcgag gttttcatcg tcaggaagcc
    12721 gcaaattaag caatcagcaa ccgttttcgt ggctttacac ttataagggt gttaagaagc
    12781 ccatcagtct gataaggtta agatattcat tcagtctatt tataatatta acaatcgtta
    12841 agcgtacact ctatggaaag ccctactcca cagcctgctc ctggttcggc gaccttcatg
    12901 gaaggatgca aagacagttt accgattgtt attagttata tcccggtggc ctttgcgttc
    12961 ggtctgaatg cgacccgtct gggattctct cctctcgaaa gcgttttttt ctcctgcatc
    13021 atttatgcag gcgcgagcca gttcgtcatt accgcgatgc tggcagcagg gagtagtttg
    13081 tgggttgctg cactgaccgt catggcaatg gatgttcgcc atgttttgta tggcccgtca
    13141 ctgcgtagcc gtattattca gcgtctgcaa aaatcgaaaa ccgccctatg ggcgtttggc
    13201 ctgacggatg aggtttttgc cgccgccacc gcaaaactgg tacgcaataa tcgccgctgg
    13261 agcgagaact ggatgatcgg cattgccttc agttcatggt catcgtgggt atttggtacg
    13321 gtaatagggg cattctccgg cagcggcttg ctgcaaggtt atcccgccgt tgaagctgca
    13381 ttaggtttta tgcttccggc actctttatg agtttcctgc tcgcctcttt tcagcgcaaa
    13441 caatctcttt gcgttaccgc agcgttagtt ggtgcccttg caggcgtaac gctattttct
    13501 attcccgtcg ccattctggc aggcattgtc tgtggctgcc tcactgcgtt aatccaggca
    13561 ttctggcaag gagcgcccga tgagctatga ggttctgctg cttgggttac tggttggcgc
    13621 ggcgaattat tgcttccgct atttgccgct gcgcctgcgt gtgggtaatg cccgcccaac
    13681 caaacgtggc gcggtaggta ttttgctcga caccattggc atcgcctcga tatgcgctct
    13741 gctggttgtc tctaccgcac cagaagtgat gcacgataca cgccgtttcg tgcccacgct
    13801 ggtcggcttc gcggtactgg gtgccagttt ctataaaaca cgcagcatta tcatcccaac
    13861 actgcttagt gcgctggcct atgggctcgc ctggaaagtg atggcgatta tataacccac
    13921 aagaatcatt tttctaaaac aatacattta ctttatttgt cactgtcgtt actatatcgg
    13981 ctgaaattaa tgaggtcata cccaaatgga tagttcgttt acgcccattg aacaaatgct
    14041 aaaatttcgc gccagccgcc acgaagattt tccttatcag gagatccttc tgactcgtct
    14101 ttgcatgcat atgcaaagca agctgctgga gaaccgcaat aaaatgctga aggctcaggg
    14161 gattaacgag acgttgttta tggcgttgat tacgctggag tctcaggaaa accacagtat
    14221 tcagccttct gaattaagtt gtgctcttgg atcatcccgt accaacgcga cgcgtattgc
    14281 cgatgaactg gaaaaacgcg gttggatcga acgtcgtgaa agcgataacg atcgccgctg
    14341 cctgcatctg caattaacgg aaaaaggtca cgagtttttg cgcgaggttt taccaccgca
    14401 gcataactgc ctgcatcaac tctggtccgc gctcagcaca acagaaaaag accagctcga
    14461 gcaaatcacc cgcaaattgc tctcccgtct cgaccagatg gaacaagacg gtgtggttct
    14521 cgaagcgatg agctaacgcg tcatctcgct caaaaatcca gatttataaa agaaaaaatg
    14581 accggccagc atcgcaacat gctggccttt ttggcaagca ggtcggctca gccgatgagt
    14641 taagaagatc gtggagaaca atatgagcgc aaatgcggag actcaaaccc cgcagcaacc
    14701 ggtaaagaag agcggcaaac gtaagcgtct gctcctcctt ctcaccttgc tctttataat
    14761 tattgccgta gcgataggga tttattggtt tttggtactg cgtcacttcg aagaaaccga
    14821 tgacgcatac gtggcaggga atcaaattca aattatgtct caggtgtctg gcagcgtgac
    14881 gaaagtctgg gccgataaca ccgattttgt aaaagaaggc gacgtgctgg tcactctcga
    14941 cccgacagat gctcgccagg cgtttgaaaa agccaaaact gcactggctt ccagcgttcg
    15001 ccaaacccac cagcagatga ttaacagcaa gcagttgcag gcgaatattg aggtgcaaaa
    15061 aatcgccctc gcgaaagcac aaagcgacta caaccgccgt gtgccgctgg gcaatgccaa
    15121 cctgattggt cgcgaagagc tgcaacacgc ccgcgacgcc gttaccagtg cccaggcgca
    15181 actggacgtc gcgattcaac aatacaatgc caatcaggcg atgattctgg ggactaaact
    15241 ggaagatcag cctgccgtgc aacaggctgc caccgaagta cgtaacgcct ggctggcgct
    15301 ggagcgtact cgtattgtca gtccgatgac cggttatgtc tcccgccgcg cggtacagcc
    15361 tggggcgcaa attagcccaa cgacgccgct gatggcggtc gttccagcca ccaatatgtg
    15421 ggtggatgcc aactttaaag agacgcagat tgccaatatg cgtatcggtc agccggtcac
    15481 tatcaccacc gatatttacg gcgatgatgt gaaatacacc ggtaaagtgg ttggtctgga
    15541 tatgggcaca ggtagcgcgt tctcactgct tccggcgcaa aatgcgaccg gtaactggat
    15601 caaagtcgtt cagcgtctgc ctgtgcgtat cgaactggac cagaaacagc tggagcaata
    15661 cccgctgcgt atcggtttat ccacgctggt gagcgtcaat accactaacc gtgacggtca
    15721 ggtactggca aataaagtac gttccactcc ggtagcggta agcaccgcgc gtgaaatcag
    15781 cctggcacct gtcaataaac tgatcgacga tatcgtaaaa gctaacgctg gctaatccag
    15841 aggtgcgtgt gatgcaacag caaaaaccgc tggaaggcgc gcaactggtc attatgacga
    15901 ttgcgctgtc actagcgaca ttcatgcagg tgctggactc caccattgct aacgtggcga
    15961 tccccactat cgccgggaat cttggctcat cgctcagcca gggaacgtgg gtaatcactt
    16021 ctttcggggt ggcgaatgcc atctcgatcc cgcttaccgg ctggctggca aagcgcgtcg
    16081 gggaagtgaa actgttcctc tggtcaacca tcgcctttgc tattgcgtcg tgggcgtgtg
    16141 gtgtctccag cagcctgaat atgctgatct tcttccgcgt gattcagggg attgtcgccg
    16201 ggccgttgat cccgctttcg caaagtctat tgctgaataa ttacccgcca gccaaacgct
    16261 caatcgcgct ggcgttgtgg tcgatgacgg tgattgtcgc gccaatttgc ggcccaatcc
    16321 tcggcggata tatcagcgat aattaccact ggggctggat attcttcatc aacgtgccga
    16381 ttggcgtggc ggtggtgttg atgacactgc aaactctgcg cggacgtgaa acccgcaccg
    16441 aacggcggcg gattgatgcc gtggggctgg cactgctggt tattggtatc ggcagcctgc
    16501 agattatgct cgaccgcggt aaagagctgg actggttttc atcacaggaa attatcatcc
    16561 ttaccgtggt ggcggtggtg gctatctgct tcctgatcgt ctgggagctg accgacgata
    16621 acccgatagt cgatctgtcg ttgtttaagt cgcgcaactt caccatcggc tgtttgtgta
    16681 tcagcctcgc gtatatgctc tacttcggcg cgattgttct gctgccgcag ttgttgcagg
    16741 aggtctacgg ttacacggcg acctgggcag gtttggcctc tgcgccggta gggattattc
    16801 cagtgatcct gtcgccgatt atcggccgct tcgcgcataa actggatatg cgccgactgg
    16861 taaccttcag ctttattatg tatgccgtct gcttctactg gcgtgcctat acctttgaac
    16921 caggtatgga ttttggcgcg tcggcctggc cgcagtttat ccaggggttt gcggtggcct
    16981 gcttctttat gccgctgacc accattacgc tgtctggttt gccaccggaa cgactggcgg
    17041 cggcatcgag cctctccaac tttacgcgta cgctggcggg gtctatcggc acgtcgataa
    17101 ccacaaccat gtggaccaac cgcgagtcga tgcaccatgc gcagttgact gagtcggtaa
    17161 acccgttcaa cccgaatgcc caggcgatgt acagtcaact ggaagggctt gggatgacac
    17221 aacagcaggc atcaggctgg attgcccagc agatcaccaa tcaggggctg attatttccg
    17281 ccaatgagat cttctggatg tcggccggga tattcctcgt cttgctgggg ctggtgtggt
    17341 ttgctaaacc gccctttggc gcaggtggcg gcggaggcgg tgcgcactaa gtacaactaa
    17401 gccagttcat ttgaactggc ttttttcaat taattgtgaa gatagtttac tgactaaatg
    17461 tgcagttcct gcaacttctc tttcggcagc gccagttctt cgttgctgtt gatgcgcacg
    17521 tcacgttcca gaatgctacg cgcaatatcc tgcgcttcct gcaacgagtg catctggtaa
    17581 gtgccacact ggtagacgtt cagctccgga atctgattct gatcctgcac tttcagcacg
    17641 tcttccattg ccgctttcca gacatcagca acacgctgct catctggcgt accaatcaga
    17701 ctcatataaa aaccggtgcg gcagcccatt ggcgagatat cgataatctc tacaccatta
    17761 ccgttaagat ggttacgcat aaaaccagca aacaggtgct ccagggtatg gatccctctt
    17821 tctggcatca cttctttgtt cggcacgcag aagcgcagat cgaacacggt gattgcgtcg
    17881 ccatgcgggg tgttcattgt tttcgccacc cgaactgcag gcgcttccat ccgggtatga
    17941 tcgactgtga agctatctaa caacggcatt tagccacctc cggtaatttt tttaaaaatt
    18001 ttctgaactc tttcttccca ggcgagtctg agtatatgaa agacgcgcat ttgttatcat
    18061 catccctgaa ttcagagatg aaattttggc cactcatgag tggccttttt cttttctgtc
    18121 aggcgtgttt ttccagccac accgcaaacg gttcggtatc tgcggcttcc atttcctgct
    18181 gacggcgttc agatgcctcg cgctcggcta caaaatcctc ttcgcgcaga atttccagcg
    18241 gctcttcacg cagcagatta cggtacgctt cagcaaacgc tttgcctgtt ccgccaatac
    18301 cagtatcaat catagacctt aagatacggg cagagaaagt cagatcggga ttatcgaagc
    18361 aggcaaccag ttcatcacac actttctgat acgcttcgcc gccgttaatg ctatccagcg
    18421 tttgcgcgac gcgtttcaga tcgcggaaca gatctttacc cacctgcggt aacgggaact
    18481 gtgcggtttc gcagccgata cccagcgtca gacccggttt gcgaccttcg aggatcaccc
    18541 ggttccagtt aacgcgtgta caggcaagtt cgctactgct catttccggt gcatcagcca
    18601 gcgcacacca gaccataaac aggtcgagga atcgcacctg ctgttcatct acaccaatcg
    18661 gcgagaacgg gttgatgtcc agcgaacgca cttcaatata ttcaatgccg ccacgtaaca
    18721 gcgcatcaga aggcgactcg ccgctgcggg taacgcgttt tggacgaatc ggcgcgtaca
    18781 gttcgttttc aatctgcagc acgttgctgt tgatttgcag cctcttaccg tctttatcaa
    18841 taccaatctt cgcgtactct tccgatggcg ttttgattgc ctgtttcagg cccgctacgt
    18901 actcgtaaag atcgttgaag gtaataccaa gattgctttg cgatttattg gtatagccga
    18961 gatcgctcaa acgaagagag gtcgcatacg gcaggtaata cataccgcac tcggttttct
    19021 caaacggcag cgacgttggt ttaccttgca ggaatgaaga gcatattgcc ggagacgcgc
    19081 caaacagata aggaatgacc caaccgaagc gatagtagtt gcgaataacg cggaaatagc
    19141 ccgcagagat tttctcttta gcatcagcgc ccgaaatatc gccacacttc gcttgccaga
    19201 atgccatagg caaagagaag ttgtaatgca cgccggagat ggtttgcatc agcgcgccgt
    19261 agcgattttt cagcccttca cggtacagcg ttttaaagcg tccggtgtta gaagtgccgt
    19321 actgcgccag ttcgatgtcc tgaccttctg cgatgtagca tggcatactt aacggccaca
    19381 tccgctcatc gcccatattg cgcgccgtat aacgatgcag atcgcgcata aaggtcagca
    19441 tatgttcaat atcaccatcc actggtgtaa tgaattccag caatgcttcc gcaaaatcgg
    19501 tagtaatcca tttatgcgtc agtgcggaac ctaatgcttc aggatgacct gttgttgcca
    19561 gtgtgccatc agcattaaca cgcaaagttt cgcgctccag cccacgctgt atccccttta
    19621 acgcctgagg atgtttttcc agccaggcca gcgcctgtga tacgtccggg atcaaattga
    19681 cctcccgcct gtcaaaatcg ttttaattag cataactgta atggtgacca tatgtgcagg
    19741 cctacaatta gtgccaccac atcatgccct gaacggtcgc tgctgcaact gcaacatagc
    19801 gtaacgcttt accaaggcac aaaaaaaaga ttaccggccc ccaggagatg cgcatccatc
    19861 ccgctaacag acacagtaaa tcgccaacca cgggcatcca gcttaataat agcgtgactg
    19921 caccatagcg tttcagccag ccggtagctt tctcttgcca gcgcgatgtt ttacgcaatg
    19981 gacagaaacg cccaaggata acgttagtta accctccaag gctattaccc attgttgctg
    20041 ttaaaactaa aacccaggga tgactgatcc cagaaagcaa cattgccacc agcacgactt
    20101 cggagttgcc gggtaatagt gtagcgctga gaaaactact ggcaaacaac gagaaaagcg
    20161 ataacgcttc actcacagca agcgaacatc cacagcgtcc atgcctgctg cacgggccgc
    20221 ctgaataccg aaatcggcat cttcaaagac cacacactgc gtcggttgca cgcccatacg
    20281 ctgcgcgcac aacaaaaatg tgtctggcgc gggtttatgg tgtttgacgt gatcggcagc
    20341 gacgacggcg tcaaaataac ggcgtaatcc caggtgagcc agcaatgcct cagcgatggc
    20401 gctttcactc cccgttccta cagccattgg gcgacgacca tgccaacttt tcaccacttc
    20461 aacaagagga agcggctcga cactatccag cagcatactt cttactgctt ctgttttttc
    20521 acgcgctaac gcatgcgggt cgagatcggc ctgattcagc tcaataattg cctgagcaat
    20581 acgccaggtg ggcgatccat taagcgcaat catcgcctga acatcgtact gaagaccgta
    20641 gtgccctaat acttcgcgcc acgctttacg gtgcgtaggc tccgtatcca ggattgtgcc
    20701 atccatatca aaaattaaac ctgcataacg ctcgtacatg gtcttctcgc aagtcgaaaa
    20761 atatgacgct actttagcgt aattgcttga ttttgtcgct gatggcgaag gagaagaaaa
    20821 ttcaggaaat ggataaagta gttatttaga aatgagatat ttttgaagga aattttttgg
    20881 agaagatggt gcatccggga ggatgactca ctggcgttcg cccttcgggc cgttgctaaa
    20941 gcaacgttat ccttcctggt gcctgcggtt aattccgcta cctcttcgat atcttcattg
    21001 ctgaagattt cggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
    21061 gtagccgagt actctatcca gctgagctac ggatgcatcg ggaaaattta ttttactgct
    21121 gatattgata tcgctactaa agccatatca aataagaaaa tccatcttaa ccatcatcac
    21181 tgatgattac ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg
    21241 tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaatttatt ttactgctga
    21301 tattgatacc gctactaaag ctgtatcaag taagagatgg tgcatccggg aggattcgaa
    21361 cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcatcggg
    21421 aaacttactt tactgcagat tttttgatac cgctactaaa gccgtatcaa gtaagagatg
    21481 gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag
    21541 ctgagctacg gatgcaaatg gcggtgaggc ggggattcga accccggatg cagcttttga
    21601 ccgcatactc ccttagcagg ggagcgcctt cagcctctcg gccacctcac cacacgcctc
    21661 ttacgagtgc ttcgaagaac ttgtttatcg ctcatcgtcg ctgcgtggcg cacatattac
    21721 tttctgagac ttataagtca aacaattttt cccacacttt tatcgtttgc acacttcacg
    21781 ctcaattagt ctgtaaaaac gtcaaaaaga gtgttttatc tacagaaaaa cggaggtctg
    21841 gcagatagca gtaatgcaaa aaaatggaga cttaagttga atgaacggga gtaaagcgaa
    21901 aagactatag agtgaaggag aaattttgag ggtgcgtctc accgataaag atgagacgcg
    21961 gaaagattag taactggact gctgggattt ttcagcctgg atacgctggt agatctcttc
    22021 acggtgaaca gaaacttcct tcggggcatt tacgccaata cgtacctggt tgccctttac
    22081 ccctaaaact gtcacggtga cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt
    22141 cagaatcagc attctttgct ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg
    22201 cattatccat catataacgc caaaaagtaa gcaatgacaa acacattaca tgtaagcagt
    22261 catggcatta cattctgtta aacctaagtt tagccgatat acacaacttc aacctgactt
    22321 tatcgttgtc gatagcgtta atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg
    22381 cttatattat tgcaatttcg cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa
    22441 ggccgcagca tccgtaccac cggcttgcgc catgtcagga cgtccaccac ccttgccgcc
    22501 cacctgctga gcgaccatac caatcagttc ccctgctttc acacgatctg tgacgtcctt
    22561 agatacgcct gcaatcagag aaaccttacc ttcggctacc gttgccagca cgataattgt
    22621 cgaccccagc tgatttttta aatcgtcaac catggtacgc aacattttcg gctcaacacc
    22681 gctaagctcg ctaaccaaca gcttaacacc attaacatca attgccttac tggaaagatt
    22741 tgcgctctcc tgtgcggcag cttgttcttt aagctgttgt agttcttttt ctaactgacg
    22801 cgtacgttcc agtactgagc gcactttatc cgccagatta ttgctatcgc ctttcagcag
    22861 atgcgcgact tcgcttaagc gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc
    22921 ggttaccgct tcgatacgac gaacgcctgc agcagtaccc gattcagaga tgatgcggaa
    22981 cagaccaata tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg tggagaaatc
    23041 gcccatgctc agcacgcgta cacgctcatc atacttctcg ccgaacagcg ccatcgcacc
    23101 tttcgctttc gccgcttcga gatccatgat gttggtttcg atcggcaaat tgcgacgaat
    23161 ctgtgcgttc accaggtctt cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg
    23221 tgagaagtcg aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt
    23281 ccccagaacc tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat tcaaacgaat
    23341 acgggcgcga cgagcctcat caacatcagc ctgcacagcg tcgcccactt tcagagaacc
    23401 cgcagcaagt ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat cttccaccac
    23461 gaaggagaag ttagcgcctt tcagttcgcc tttatcacca acctgaccgc cggattccgc
    23521 atagaatggc gtttgatcca gcacgaccac agcttcctgg cctgcattga tggcatcaac
    23581 cgctttacca tcaacaaaca gcgcggtcac tttgccgttc agttccagat ggtcatagcc
    23641 tttaaattca gatgcactgt caacacggat cattgcgttg taatcggcac caaagccgct
    23701 ggcttcgcgc gcccgacgac gctgctcttc cattgctgct tcaaaaccag cttcgtcaac
    23761 tttgatgttg cgctcacgac aaacatcagc cgtcaggtca accgggaagc cataggtgtc
    23821 gtacagacgg aaagcagttt caccatccag cgtatcacca gaaagttttg ccagctcttc
    23881 atccagcaac gccagaccgc gctccagagt acgagcaaac tgctcttctt cagtcttcag
    23941 cacctgctca acctgcgcct gctggcgttt caggtcttca cccgcagagc ccataacgtc
    24001 gatcagcgga ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca tattaccgtg
    24061 acgcactgcg cgacgaatga tacgacgcag tacataacca cggttttcat tggacggcat
    24121 tacgccatcc gcgatcagga acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga
    24181 tttattgctc agatcggttg cgccagtgac tttcgctacc gcctggatca acgtgcggaa
    24241 caggtcgatg tcatagttag agttaacgtg ttgcagcacc gcagcaatac gctccagacc
    24301 cataccggta tctacagacg gcttcggcag cggttccatc gtgccatcgg cctggcggtt
    24361 gaactgcatg aagacgatgt tccagatctc aatgtagcgg tcgccgtctt cttccggact
    24421 tcccggaggg ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc
    24481 gcacggacca gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg cacctttgtt
    24541 atcgccgatg cgaataatac gttcgcgcgg gatccctact tctttttccc agatttcgta
    24601 ggcttcgtcg tcgctttcat agacggtaac ccacagacgc tctttcggca gggcaaacca
    24661 tttttcgctg gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc
    24721 gaagctgaag ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt
    24781 ttccaggtcg ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga
    24841 ataattacgc ttgtcgagcc caaggaacac atccttgaac tggttcatcc cggcgttggt
    24901 aaacaacaaa gttgggtcgt tatggggtac cagggagctg ctggcaacta cctgatgtcc
    24961 cttactatgg aaaaagtcga gaaacgcctg acggatctca gcggtgctct tgctcataat
    25021 tatcctgaaa tcaagctaac gaaatatcgc caccagctcc agcgtgtctt aaccgccggg
    25081 ctggtaactg aaaagtggga ataagataag ttttcttgac tgggaagtaa aataccgtat
    25141 gcgttcagtc ggcaaaattt cgccaaatat cctggatatc ttccatcaga tagccacgat
    25201 agagcagaaa acgctggatc ttaacttttt ctgaaaagac agttggcaaa ggttcaccat
    25261 attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg
    25321 ctttttctgt cgcttcgcgg gaaatacctt tctgattcag ttcctggcga atacgcgcag
    25381 gtccataacc tttgcggcta cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga
    25441 gatagccatg ttcatggcac caggcaataa cgcgctcgta atcttctgcc gtagcatcaa
    25501 tctcttctgg gccatttttg cccataatcg gtgccgcgag tttacgtcgc agttcttgct
    25561 cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga gcatatgccg
    25621 ggcgacggga cgttgattct gtcatggcat atccttacaa cttaaaaagc aaaagggccg
    25681 cagatgcgac ccttgtgtat caaacaagac gattaaaaat cttcgttagt ttctgctacg
    25741 ccttcgctat catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca
    25801 cgtactttct tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagttgca
    25861 ttcgctttac cctgaccgat cttctcacct ttgtagctgt accacgcgcc tgctttctcg
    25921 atcagcttct cttttacgcc caggtcaacc agttcgccgt agaagttgat accttcgccg
    25981 tagaggatct ggaattcagc ctgtttaaac ggcgcagcga ttttgttctt caccactttc
    26041 acgcgggttt cgctacccac cacgttttcg ccctctttca ccgcgccgat acgacggatg
    26101 tcgagacgaa cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta
    26161 ccgaacatca caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac
    26221 tgcttcaggt tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc
    26281 atgtgagagt cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag
    26341 tcaacgacga taacgtctac tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc
    26401 tgctcgccgg tgtccggctg ggagcacagc aggttgtcga tatcgacgcc cagtttacgt
    26461 gcgtagattg ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcacgc
    26521 tgcgctgcgg caatcacctg caacgtcagc gtggttttac cggaagattc cggtccgtag
    26581 atttcgacga tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc
    26641 gaaccggtag agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag
    26701 cctttaccaa attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt
    26761 tcgtcgatag ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct
    26821 gttgaagcaa ttatactgta tgctcataca gtatcaagtg ttttgtagaa attgttgcca
    26881 caaggtctgc aatgcatacg cagtagcctg acgacgcacc gcatcacggt cgccgctgaa
    26941 gcattcccgc agggtaatgc cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc
    27001 gacaggcttc tcttcactgc cgccatccgg cccggcgata ccactaatag acacggcata
    27061 atcagcacga gccgctttca gtgcacctat cgccatttcc accacgacgg gttcactcac
    27121 cgcgccatgc tgcgccagcg tctcttcgcg tacgccgatc atttgcgctt tggcttcgtt
    27181 actgtaggtg acaaatccgc gttcaaacca ggcggaactg ccggcaatat cggtaatcac
    27241 tttcgctacc caaccgccgg tacaagactc agcagttgtt acggttgcgc cacgggcttt
    27301 cagcgcctgc ccaacctgtt cgcttaactg catcagttca ctgtcagtca tcacaacctc
    27361 tgtcagtcaa aaatttatgc cggacaagat agcactttcg tcaggaagat ggtgttggcg
    27421 ttgcaatttt acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgccc
    27481 ccctcttgca aatagttact gcacgcgcgc cagcgccacg gcttgtccta actgccagac
    27541 cgccattgcg taatgggtac tgtggttata acgggtaatg gtgtagaagt tcggcagacc
    27601 gtaccagtac tggtaacctg ttccaacatc cagacgcagc aggctggctt gttgatggtt
    27661 gcccagcggc tgctgtggcg ttaaacctgc cgcggcaagc tgcgaaatgc tgtacctggt
    27721 tttgaagcca tttggcaagc ctggagcctg accgtttgcc attaccgcga cctgatcgcc
    27781 tttcacccag ccgtgcgctt tgaaataatt cgccacgcta ccgatcgcat caaccggatc
    27841 ccacaggttg atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg
    27901 cataaactgt ccgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc
    27961 ctgctcgtcg cgcgccatca gcaggaaggt ttccagctcg ccagagaaat actccgcgcg
    28021 gcgtgggtag ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt tccccatcac
    28081 gcgcccccag cgggtttcaa cgccgataat cccgacgata atttccggcg gtacgccata
    28141 cacctgccac gcgcgattca acgcatcttc atactgattc cagaaaacca caccgttctg
    28201 cacattgtcc ggcgtaataa attttttgcg ataacgcagc catgcgccgt tcggacctga
    28261 tggcggtttc accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag
    28321 acgtttcgcc tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac
    28381 cattctgtcg atgaactgct gggcattcgg gttattagcg aaatcgccgc ccatctgcat
    28441 cacattgtgc tgcggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtttcagt
    28501 ctcagtaggt tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt
    28561 tacataacga cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg
    28621 gtaaagcatc acgccccgca caaggaagcg gtagtcactg cccgatacgg actttacata
    28681 actcaactca ttctcctcgc tatcctttta ctcaaacttt caaattaaaa catttatctt
    28741 tcattttgcg atcaaaataa cactttaaat ctttcaatct gattagatta ggttgccgtt
    28801 tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccatt actcatggtg
    28861 cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccggg atggtgaccg
    28921 gcattcttcc gctgttgatt agcctgctgg ttatcatgaa cgcactgatt aattttatcg
    28981 gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt tcccgttacc
    29041 tactgttacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta agcctgggtc
    29101 gctttatgcc ggaaaagtac aaacccagct actacgcggc ggcctcttat agctgccact
    29161 caatgaatgg cctcttcccc catatcaacc ctggcgaact gtttgttatc ttggcattgc
    29221 cagcggtctg acaacgctga acctgccact tggcccactg gcggtgagct atctgctggt
    29281 tggtctggtc accaacttct tccgcggctg ggtgaccgat ctgaccaccg ctatttttga
    29341 gaaaaagatg ggcattcaac ttgaacaaaa agttcatctg gcaggagcaa catcatgacg
    29401 catattcgga tcgaaaaagg aacggggggc tggggcggcc cgcttgagct ggaaaccacg
    29461 ccgggcaaaa aaatcgtcta tatcaccccg ccggtacccg gcctgcgatt gtcgacaaac
    29521 tggcacagct tactggctgg caggctattg acggatttaa agaaggtgaa cccgcggagg
    29581 cggaaattgg tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atttatccga
    29641 aacggcgtat tcccaccatt aatatccact cgacgggcaa gtccggtcca ctggcgcagt
    29701 acattgtgga agatatttat gtctctggcg taaaagaaga aaacatcact gtagtgggtg
    29761 atgcgacacc acaaccctct tccgtgggcc gtgactatga caccagcaag aaaatcaccg
    29821 aacaaagcga tggtttactg gcgaaggtgg gaatgggtat gggttctgcc gttgccgtgt
    29881 tgtttcaatc tggtcgtgac accatcgaca ctgtattaaa aaccattctt ccgtttatgg
    29941 cattcgtctc ggcgctcatt ggcatcatta tggcttctgg ccttggtgac tggattgccc
    30001 acggccttgc tccgctggcg agccatccac tgggtctggt catgctggcg ctcatctgct
    30061 ccttcccgct gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg
    30121 tattgattgg cgtgcagatt ggtctcggca atattccgcc gcatctggct ttacctgcac
    30181 tgtttgccat caacgcgcag gcggcctgcg acttcattcc ggtcggtttg tcgctggcgg
    30241 aagcccgtca ggacacggtt cgcgtcggtg tcccttctgt actggtgagc cgctttttaa
    30301 ccggcgcgcc gactgtactg atcgcctggt ttgtctccgg ttttatctat caatagaggc
    30361 tgaaacatga ccgttattta tcagaccacc atcacccgta tcggcgcgag tgccactgac
    30421 gccctcagcg accagatgct catcaccttt cgtgaaggcg cgccagcgga cctcgaagag
    30481 tattgcttca ttcattgcca cggcgagttg aaaggtgcac tccatcccgg tttgcaattt
    30541 acgctcgggc agcatcgcta tccggtgacc gctgttggca gcgtggcgga agacaacctt
    30601 cgcgaactgg gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga atttccgggc
    30661 actgtccatg tggcaggccc tgtacccgac gatatcgcgc cgggatcggt tttgaagttt
    30721 gaatctgtta aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc
    30781 ttaggcgcgt tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat
    30841 attcagagcg acaaagccgc aaatgtggca caagaaatta acgccgaata tggtgaagga
    30901 acagcgtatg gttttggtgc tgacgccact agcgagcaaa gcgttctggc gctctctcgt
    30961 ggggtagatg aaatctttgt tcgtgtggat ttactggtct acagcgccgg aattgcaaaa
    31021 gcagccttta tcagcgactt ccagctcggc gattttgacc gttcgctaca ggtgaatctg
    31081 gtgggttatt tcctgtgtgc gcgtgaattt tcgcgtttga tgatccgcga tgggattcag
    31141 ggacgcatta ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca caattctggt
    31201 tatagcgccg cgaaatttgg tggcgtaggg ctgactcaat cactggcgct ggatctggcg
    31261 gagtacggca ttacggtgca ttcactgatg ctcggtaacc tgctgaaatc gccgatgttc
    31321 cagtcgctgt tgccacaata tgccaccaag ctgggtatca aaccggatca agtcgagcag
    31381 tattacatcg acaaagtacc gctcaaacgc ggctgcgatt atcaggatgt gctgaatatg
    31441 ctgctgttct acgccagtcc taaggcgtcg tactgtaccg gacagtcgat caatgtcacc
    31501 ggcggtcagg tgatgttctg atcaacagcg gagatccgtt aaggatctcc gtgagactaa
    31561 tagaatgcct gatgcgctac gctcatcagg catacaggac ttccgccact acattaagga
    31621 aaagttatgg tatccgcact catcactgtc gccgttatcg cctggtgtgc gcaactggcc
    31681 ttaggcggct ggcaaatttc tcgttttaac cgtgccttcg acacactatg ccagcaaggg
    31741 cgggttggcg tgggccgttc cagcgggcgc tttaaaccgc gggtcgtggt cgccatcgcc
    31801 ctggacgatc agcagcgcat cgccgacacc ttgtttatga aaggactgac cgtcttcgcc
    31861 cgaccgcaaa aaattcccgc aattaccggt atgcatgcgg gtgatttaca gcccgatgtg
    31921 atctttcccc atgatccact atcacagaat gctctatcat tggcgcttaa actgaaacgt
    31981 ggataatttc gttgtgaatg ttacttgctt gcgaagttat cattttgaaa cctaaatcag
    32041 gtaatcacgc ccatgaaacc tcgtcagcgt caagccgcca ttctggagta tctgcaaaag
    32101 cagggaaaat gctcggttga agaattggcg caatattttg acaccacagg cacaactatt
    32161 cgcaaagatc tggtcattct ggaacatgcc ggaaccgtca ttcgtactta tggcggagtg
    32221 gtgttgaata aagaggaatc cgatccgcct atcgatcata aaacactcat caacacccac
    32281 aagaaagagc tgatagcaga agctgccgtt ggttttattc atgatggcga ttcgatcatt
    32341 cttgatgctg gcagtaccgt tttgcagatg gtgcctctgc tctcgcgctt taataacatc
    32401 acggtgatga ccaacagcct gcatatcgtc aatgcgttat ctgaactgga taatgaacaa
    32461 actatcctga tgccaggcgg aacgtttcgt aaaaaatcgg cctcatttca cgggcagctg
    32521 gcagagaatg ccttcgagca tttcaccttc gacaaattat tcatgggtac cgacggcatc
    32581 gatctcaatg cgggcgtaac caccttcaac gaggtctata ccgtcagtaa ggcaatgtgc
    32641 aatgccgcgc gcgaagtgat tttgatggcg gactcatcaa agtttggccg taaaagcccc
    32701 aacgtagttt gcagtcttga aagcgtcgat aagctgatta ccgacgcaga tatcgatccg
    32761 gcgtttcgtc aggcgctgga agagaaaggg atcgatgtga tcataaccgg agagagcaat
    32821 gagtgaagca ctactgaacg cgggacgtca gacgttaatg ctggaattgc aggaagcaag
    32881 ccgtttaccg gaacgtctgg gcgatgattt tgttcgcgcc gccaatatca tcctgcactg
    32941 cgaaggcaaa gtggtggttt cgggaattgg caaatcgggc cacattggta agaaaatcgc
    33001 cgcaacgctt gccagtaccg gcacaccggc tttttttgtc catccggcag aagcgctgca
    33061 tggcgatctg gggatgatcg aaagccgcga tgtgatgctg tttatctctt actccggtgg
    33121 ggcgaaggaa ctggatctga ttattccgcg tctggaagac aaatctatcg cgctgctggc
    33181 gatgaccggc aaaccgacgt caccgctggg cctggcggcg aaagcggtgc tggatatctc
    33241 cgtagaacgc gaagcctgcc cgatgcacct tgcgccgacc tccagcaccg tcaataccct
    33301 gatgatgggt gacgcgctgg cgatggcggt catgcaggcg cgcggattta atgaagaaga
    33361 ttttgcccgc tcccaccctg ctggagcact gggcgctcgc ttgctgaata aagtgcatca
    33421 tctgatgcgc cgtgacgatg ccatcccaca ggtggcgtta accgccagcg tgatggatgc
    33481 tatgctggaa ctcagccgca ccggtctggg gctggtggcg gtatgtgacg ctcaacaaca
    33541 ggtacaaggc gtctttaccg acggcgattt acgtcgctgg ctggttggcg gcggcgcact
    33601 caccacgcca gtcaatgaag cgatgacggt cggcggcacc acgttgcaat cgcaaagtcg
    33661 cgccatcgac gccaaagaga tcctgatgaa gcgcaaaatc actgccgcac cggtggtgga
    33721 tgaaaacggc aaactcaccg gcgcaataaa cctgcaggat ttctatcagg ccgggattat
    33781 ttaatccttc aatcccagac gtttcgccag ccgatgcagg ttggcgacgt cggtttccag
    33841 catccgcgcg caggcagccc agttgtgatg attttgtgcc agtgcctgac gaatagtttc
    33901 acgctggaac gcttctgtcg cttcacgcag gttttgctta acaacgggca ccgccgccac
    33961 ttctggcggc ggcaacgtca cctcaggaaa agcaaaatgt tgcgcctcaa gaatcacttc
    34021 atcgccgctg cgggtggctc tcgccagcac taccgcccga tgaatagcat gttccagttc
    34081 gcgcacgttt cccggaaaac tgtagtgttg cagtaaattt cgcgccccgg cacttaatac
    34141 cacgcgggag agcccctgcc gcaaacgaca ctgctcgcag aaataccccg ccagcagaat
    34201 gacatcatcg ccccgctcac gcagcggcgg caccgaaagt ggaaacacac tcaggcgatg
    34261 aaacaaatcg gcgcggaatc gccctgccag cacctcttcg cgtaagtcgc ggttagtcgc
    34321 cgccagcacg cgcacatcga cccgcaaact acggtcatcg ccaacgcgct gaatatcgcc
    34381 atactgcaac accctcagca gcttggcctg caatgccaac gacaactcgc cgatctcatc
    34441 gagaaacagc gtgccgttat ccgccatttc aaacttccca ctgcggttac tgatagcgcc
    34501 agtaaacgct cctttcacat gcccgaacaa ctcactttcc gccacacttt ccggcagtgc
    34561 ggcacagttg agatagacca gcggattcac cgcccgtggc gaggcctcat gaatcgcttt
    34621 cgccaccagc tccttaccgg ttcccgtctc accgctgatc aggacgttga gatcggacgc
    34681 cgccacaatc tcaatctctt ttttcagttg cgtcatgcct ggcgacagac cgatcatctg
    34741 cgtctgtttc accgcttcaa atggagcggc atcgcccggc agcatattct ggctttccag
    34801 ttgttcaatc agcaacgcat tgcttaacgc tcccgccgcc agcgcggcaa tcaggcgtaa
    34861 ctcttcgtcg ctgaaaacat cgaactgatc gggctgcatc ccgtcgagcg tcaatgcgcc
    34921 gatcaggttt tgcccggcaa acagcggcag accaacgcag gcgtgaacct tcagactctc
    34981 ctgccctgga atcaaaccgt catagggatc gggcaattcg ctgtctgcgg gaaagcgcac
    35041 cacatcaccc gcgcgggcaa tcgcttccag ccgtggatga ccttccagcg caaagcgtct
    35101 accgagtaca tccttcgcca gcccgtcgat ggcaagcgga ataaactgcc gcgagtcgta
    35161 acgtagcaac gcagacgcat cgcactccag cacctgacgc agcgtggtga tcaggcgctg
    35221 aaaacgatcc tgatgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc
    35281 aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc
    35341 attggattga tagtcatttt gactactcat taatgagcat aattttattt atagagtaaa
    35401 aacaatcaga taaaaaactg gcacgcaatc tgcaattagc aagacatctt tttagaacac
    35461 gctgaataaa ttgaggttgc tatgtctatt gtggtgaaaa ataacattca ttgggttggt
    35521 caacgtgact gggaagtacg agattttcac ggcacggaat ataaaacgct gcgcggcagc
    35581 agctacaaca gctacctcat ccgcgaagaa aaaaacgtgc tgatcgacac cgtcgaccat
    35641 aaattcagcc gcgaatttgt gcagaacctg cgtaatgaaa tcgatctggc ggatatcgat
    35701 tacatcgtga ttaaccatgc agaagaggac cacgccgggg cgctgaccga actgatggca
    35761 caaattcccg atacgccgat ctactgtaca gccaacgcta tcgactcgat aaatggtcat
    35821 caccatcatc cggagtggaa ttttaatgtg gtgaaaactg gcgacacgct ggatatcggc
    35881 aacggcaaac agctcatttt tgtcgaaaca ccaatgctgc actggccgga cagcatgatg
    35941 acttacctga caggcgacgc ggtgctgttc agtaacgatg ctttcggtca gcactactgc
    36001 gacgagcatc tgttcaacga tgaagtggat cagacggagc tttttgagca gtgccagcgt
    36061 tactacgcca atatccttac gccgttcagc cgtctggtaa cgccgaaaat taccgagatc
    36121 ctgggcttta acttaccagt cgatatgata gcgacctctc acggcgtggt atggcgcgat
    36181 aacccgacgc aaattgtcga gctgtacctg aaatgggcgg ctgattatca ggaagacaga
    36241 atcaccattt tctacgacac catgtcgaat aacacccgca tgatggctga cgctatcgcc
    36301 caggggattg cggaaaccga cccacgcgtg gcggtgaaaa ttttcaacgt cgcccgaagc
    36361 gataaaaacg aaatcctgac caatgtcttc cgctcaaaag gcgtgctggt cggcacttcg
    36421 acgatgaata acgtgatgat gccgaaaatc gccgggctgg tggaggagat gaccggatta
    36481 cgcttccgta acaaacgcgc cagtgctttc ggctctcacg gctggagcgg cggtgcggtg
    36541 gatcgtcttt ccacgcgcct gcaggatgcg ggtttcgaaa tgtcgcttag cctgaaagcg
    36601 aaatggcgac cagaccagga cgctctggag ttatgccgtg aacacggtcg cgaaatcgcc
    36661 cgtcagtggg cgctcgcgcc gctgccgcag agcacggtga atacggtagt taaagaagaa
    36721 acctctgcca ccacgacggc tgacctcggc ccacggatgc agtgcagcgt ctgccagtgg
    36781 atttacgatc cggcaaaagg cgagccaatg caggacgttg cgccaggaac gccgtggagt
    36841 gaagtcccgg ataacttcct gtgcccggaa tgctccctcg gcaaagacgt ctttgacgaa
    36901 ctggcatcgg aggcaaaatg agcaacggca ttgtgatcat tggttcgggc ttcgccgccc
    36961 gccaactggt gaaaaatatt cgcaaacagg acgccagtat tccattaacc ctgattgccg
    37021 ccgacagtat ggatgagtac aacaaacctg acctcagcca tgttatcagt caggggcaac
    37081 gtgccgacga ccttacccgc cagacggcgg gtgaatttgc cgagcagttt aatctgctcc
    37141 tgtttccgca aacctgggtg acggatatcg atgccgaagc ccgtgtggtg aaaagccaga
    37201 ataatcagtg gcaatacgac aagctggtgc tggcaaccgg tgccagcgcc tttgtcccgc
    37261 cagtgcctgg gcgtgagtta atgctgacgt taaatagtca gcaagagtat cgcgcctgtg
    37321 aaacgcaact gcgggatgcc cgacgcgtgt tgattgttgg cggtggtttg attggtagcg
    37381 aactggcgat ggatttttgt cgtgcaggca aaatggttac gctaatcgac aacgctgcca
    37441 gtattctggc gtcgttaatg ccaccggaag taagcagtcg cttgcagcat cggttgacgg
    37501 agatgggcgt tcatctgctg ttgaaatctc agttgcaggg gctggaaaaa acggattctg
    37561 gcattctggc aacgctggac caccagcgca gcatcgaagt ggatgcggta attgccgcca
    37621 ccggactgcg cccggaaacc gccctggcac gacgcgccgg gctgacgatt aatcgcggcg
    37681 tttgcgtcga tagttatctg caaaccagta atgccgatat ttacgcgctg ggcgattgcg
    37741 cggaaattaa cggtcaggta ttgccgttcc tgcagccgat tcaacttagc gcgatggtgc
    37801 tggcaaaaaa tcttctcggc aataacacgc cgctgaaact accggcgatg ctggtgaaaa
    37861 tcaaaacgcc ggaattaccg ctgcatctgg caggcgaaac ccagcgtcag gatttacgct
    37921 ggcagattaa taccgaacgc cagggaatgg tggcgcgcgg tgttgacgat gcagaccaac
    37981 ttcgcgcctt tgtggtcagt gaggatcgga tgaaagaggc atttggattg ttgaaaacgt
    38041 tgccggtgta ggtgggctac tgtgcctaaa atgtcggatg cgacgctggc gcgtcttatc
    38101 cgacctacgg ggacgcatgt gtaggccgga taaggcgttt acgccgcatc cggcaatggt
    38161 gtccaaatgc aacacgtttt atccgttctg gacttcaccc gctaaccaac gcgccgcagc
    38221 aataaccccc tgccccagag acaaaccgcc atcacccgcc ggtaaactct gtggaaacag
    38281 caatgtgaaa tcagcgagat aatgcgccag acgtgcacgc agcaaacggt tatgaataac
    38341 cccgccgcta aataccagcg tagtgatacc acgcatcgtg gcctgctcac gcatcaacgc
    38401 ggcaaaaccc tgcgccagcg catcatgaaa cgcccacgcg cgttgattaa ccggtgcctg
    38461 ccagttcagc cactgctgcc agaaagtggc gagatccagt tgattgtcca cccgcggcat
    38521 cgtcaccgga tgcgtcactc cgtcgcatga ggctgctagc gcctccagag cacaagccgc
    38581 ttcaccttca taacttaacg tggctggcgc acagcccagt gccgccgcca cagcatcgaa
    38641 caaacgccca cacgatgacg ccagcggcgc gttaattcca cgctcaatgg cccgcgccag
    38701 tacgctccag ttttgctgtt gcacacttgc cgtttcaggg taattctgcc actccggcac
    38761 aaagcgcagg cactgcgcca gcaggttacg ccacggctgc ttcgccgcca aatcgccacc
    38821 cggaagcgcc actgcaggca agccgcccag gtgctcacat tcgcgatagt tcacccgcag
    38881 gcactcgccg ccccacaaag cgccgttctc ccccataccg ataccgtcga gcgtcaaagc
    38941 aatgacatca ccgccatcca gcggccactg atgctctgcc agacacgccg ctgcgtgggc
    39001 atgatgatgc agcaccgttt gcgtcggcag attcatttcg ctcgcccact ggcaggagac
    39061 atagcccgga tgtgcgtcat gcacaacgta ttgcggagtg aaattgtaga tgttttgcat
    39121 caggcgtaac gcttcgcgcc actgcgtctg gatgccatcg tcacttaaat cgcccagatg
    39181 ctggctcaac accacttgtt caccgcgcac caggctgaag gtatttttca gatccgcgcc
    39241 gagacacagt acaggcggaa catttttaaa gcccggaggc aaagccagcg catccggcac
    39301 atacccccgc gaacggcgca gcatttcgcc gctttcgcgc accaccgaat catccatccg
    39361 ctgcacaatg tcgcggttgt gtattaagaa tccgtcggca atgccctgca aatcctccag
    39421 cgcctgttcg ttgctgatag ccggtggttt accgctcagg ttgccagagg tcatcaccag
    39481 cgggcattgt agttcctgta acagcaaatg ctgaagcgga ttcgcaggca acattacccc
    39541 gacttcgtta aggccagggg cgatatcatc acaaagctca ggaacgtatt ttttatccac
    39601 cagcacaatc ggcgcggcgg gcgtggtaag caactggcgc gcagcgtctg gtaaaccgtc
    39661 agccactggc aacatgactg ccagcggttt cgccgggcga tgtttgcgcg cccgaagtgt
    39721 cgccaccgcg ttactgttac gtgcatcgca ggcaagatga aatccgccaa tccctttgat
    39781 ggcgacaatg ttgcccattt ttaactgtgc gatagccgcc tgtaatgccg cctcttgttc
    39841 tgcatgttca ccatgactta cccattcaag atacgggcca cactccgggc aggccaccgg
    39901 ctgggcgtgg aagcgacgat cgagcgggtc gcggtactct ttatcacagg ctggacacag
    39961 cggaaacgcc gccatcacgg taaacgggcg atcgtaaggc atggcgcgaa taatggtgaa
    40021 gcgcgggccg cagtgggtac agttgataaa tggataacga taacgccgtt cgcctggggt
    40081 attcatttcg gcaaggcaag cagggcaagt ggcggcatcg ggaacaattt gcgtattcat
    40141 ggcgccgccc gcgctctggc ggatggtgaa ctcagtgggc agttgtgacc agataaacgg
    40201 ttcacgctca acgctatcaa tacgcgccag cggcgggcag tgctgatgca attgaacaag
    40261 aaacgtttcc gggtcttcca gcagccggac ttctacgcca tcgccgtcat tacagacatc
    40321 gccgtgaaga tttaattgct gtgccagctg ccagacaaac ggacgaaaac cgacgccctg
    40381 cactttgcct cgaatacgca gttggacacc gcaagatgtg ttttttgcca ttgagttatt
    40441 cccgccatca tgaattgcgt aacccgtcca gccggacacg acagcgtcgc atccggcagt
    40501 cacaggtcgg cgataccgcc cgctccgtat tctacgaata tttccgggaa ttcctttgat
    40561 gccagaacag ttctgtaaga tttttagaac atcagcgccg tacggcggcg tttttctgcg
    40621 ctcagttgtt caagtttatt gcgatcgaca caaattagcg catgggtcgg gcaagccgcc
    40681 atacacgccg ggccgtcttc acgatggttg cacaggtcgc atttattggc ttcggctttg
    40741 tcagcccgta cattcagccc tgcgccgctg ttgcgaatca ccggacgtac caccacttcc
    40801 atcgcgccat acgggcaagc cacaacgcag gttttgcaac caatgcaacg ttcctgcatc
    40861 acatgaacaa accctttatc acggctgata gcaccattcg ggcagacgtt agcgcacggt
    40921 gcatcttcac actgacggca aactgtcgcc gtggaaatgt tcacaccttt aatgacatgg
    40981 atacgcggta aaaatgtttc cggggtcagc gatgcacagt cctgattttc ctgatgagaa
    41041 accacgcacg ccacttcaca ggtacggcaa ccaatacatt tactcgcgtc agcaatgatg
    41101 aaacggttca tcaaattctc cagcaatgac agttaatgcg ccgatacatt cacaaatcat
    41161 gccagttttt aatttgctgt tatttaagga aattaatttc tgtaatgcag gaaaaaagat
    41221 gtcatcgaca ctagtgacga tgacatgtga tgacaatgtt tatcgcgaaa gagcaatgag
    41281 tgagtcgcgg cggatcagtt ttccgctgaa ggttttcggc ggtgagaaat ccccgccatc
    41341 gagcataaaa atcagccgtc caataatttc ctgaatcatc tcagtcaccg gaatttttac
    41401 gctggagagc gccggaacga tatagggggc aattgcgata tcatcgaatc cgataactga
    41461 cacctgctct ggcaccgcta cgccgcgctt gtgtaacgct ttcatcgcac ctatcgccat
    41521 atcgtcgtta ctggcaacta acgcgctaaa tttagcccca cgttcgagca acgtttctac
    41581 cccttcggcc ccgctggcgg gcgtccattt accgttagcg ataagttttt cattgagcgc
    41641 aataccatgc tgcgccagcg cgtctttata tccggcaaga cgttcaacgc tggtggggga
    41701 atccatcgag ccggtaagaa aagcaatctc ctgatgcccg gcgtttatca actctgccac
    41761 ggcgttaaag ctggtctgtt tatgatcgca ccagacgcta tggctgctat ttttgcgcag
    41821 gcggcgatta agcaccatta tcggctgact gtgcgcgtca atgatgtcat cgatctcatc
    41881 cacacttaaa aaacgcgggt aaatcataat ggcgtcgcag cgcaaatcca gcagatactg
    41941 aatcgcctgg cgctcttctt cggcgctgtg tttaccatct gccaatagca actgccgccc
    42001 tttctcttcc gccattcgcg cggcatgaaa cagtaattca ctaaaataaa tgccgtggta
    42061 aagcgtgcta gtcactacca gccccagcgt ctgggtactt ttcgccgaca gattgcgcgc
    42121 cagcaagttt ggacggtaac cgctctcttc taccgcctga aacacgcgat ctttagtctc
    42181 ctggctgacg tagccattac ctgaaagcac gcgggaaacg gtcgcttttg aaaccccggc
    42241 gcgcttcgcc acttccagca tcgtcgtcat cattttcatc cctttacacg caatcaacgc
    42301 agtgtactgc accgtttacc gattgtcctt gcgcaatcgg cgggaaaaat attcaggtga
    42361 ccggtttcac aaatataaaa aatgaacaat taactctctt gcttatttgg ttacaactat
    42421 tcatgatttt gtgaaaccgg tttcttaatt ccgtttcagc atcggcattt ttccgtcacg
    42481 tcgactgata acaactacat cttccctact gataacagga taaaatccga tggccaaaaa
    42541 ttatgcggcg ctggcacgct cggtgatagc ggcactgggc ggcgttgata acatctcggc
    42601 ggtcacgcac tgtatgacgc gtttgcgctt tgttatcaaa gatgatgcgc ttatcgacag
    42661 cccgacgtta aaaaccatcc ccggcgtgct cggcgtggta cgtagtgaca accagtgtca
    42721 ggtgattatt ggcaataccg tttcacaagc ctttcaggaa gtcgtcagcc tgctgccggg
    42781 agatatgcag cccgccctgc ccgtgggtaa acccaaactc acgctgcgtc gcattggtgc
    42841 ggggatcctc gatgcgctga tcggcaccat gtcaccgctg atcccggcga ttatcggcgg
    42901 atcgatggtc aaactgctgg cgatgatcct cgagatgagc ggcgtgctga caaaaggatc
    42961 gccgacctta accattctga atgtgattgg tgacggtgct ttcttcttcc tgccgctgat
    43021 ggtcgcggct tcggccgcca tcaaatttaa aaccaatatg tcgctggcga ttgccatcgc
    43081 gggtgtgctg gtacatccga gttttattga actgatggcg aaagcggccc agggtgaaca
    43141 tgtcgaattt gccctgattc cagtcaccgc ggtgaaatac acctacacag tgatcccggc
    43201 gctggtgatg acctggtgcc tgtcatatat cgaacgctgg gtggatcgca ttacaccggc
    43261 ggtgaccaaa aacttcctca agccaatgct gattgtgttg attgccgcac cgctggcaat
    43321 cctgctgatt ggcccgattg gtatctggat cggtagcgcc atttcggcgc tggtttacac
    43381 cattcatggt tatctgggct ggctttcagt cgccattatg ggcgcgctgt ggcctctgct
    43441 ggtaatgacc gggatgcacc gcgtctttac gctaaccatc attcagacca ttgccgaaac
    43501 cggcaaagaa gggatggtca tgccgtcaga gatcggcgct aacctgtcgc tgggcggttc
    43561 atcactggcg gtggcgtgga aaacgaaaaa cccggaactg cgccagactg cgctggctgc
    43621 ggcggcatca gccattatgg cggggatttc cgaaccggcg ttatacggcg tggcgatccg
    43681 cctgaaacgt ccgcttatcg ccagtcttat cagcggtttt atttgcggcg cggttgccgg
    43741 tatggcgggg cttgccagcc actcaatggc agcgccgggg ctatttacca gcgtgcagtt
    43801 cttcgatccg gcgaatccaa tgagcatcgt ctgggtgttc gcggtcatgg cgctggcggt
    43861 ggtgctgtcg tttatcctca cactgttgct cggctttgag gatattcctg ttgaggaagc
    43921 ggctgccgag gcgcgaaagc atcagagcgc acaaccgacc gtcgccaaag aagtaagtct
    43981 taattgagga tgaaaatgtc agtatttcca gaaggttttt tatggggcgg cgcgcttgcc
    44041 gccaaccagt ctgaaggtgc gttccgtgaa ggtggtaaag gactgacaac ggtcgatatg
    44101 atcccacacg gcgagcatcg aatggcggtg aaactggggc tggaaaaacg ttttcagttg
    44161 cgcgatgacg agttttatcc cagccatgag gcgacgggtt tttatcatcg ttataaagaa
    44221 gatatcgccc tgatggcaga gatgggattc aaagtgttcc gtacctcaat tgcctggagc
    44281 cgccccttcc cgcagggtga tgaactgacg cccaaccagc agggcattgc tttttaccgt
    44341 tctgtctttg aagagtgtaa aaagtacggt atcgaaccgc tggtcacatt gtgccacttc
    44401 gatgtgccga tgcagctggt caccgaatat ggctcctggc gtaaccgcaa gctggtggag
    44461 tttttcagcc gctacgccag gacctgcttt gaagcatttg atggtctggt gaaatactgg
    44521 ctaaccttca atgaaatcaa cattatgttg catagcccgt tctccggcgc ggggctggtg
    44581 tttgaagaag gtgaaaatca ggatcaggtg aaatatcagg ccgcgcatca ccagctggtt
    44641 gccagtgcgc tagccaccaa aatcgcccat gaggttaacc cgcaaaatca ggtgggctgt
    44701 atgctggcgg gcggtaactt ctacccttac agttgcaagc cggaagatgt ctgggcggcg
    44761 ctggagaaag atcgggaaaa cctgtttttt atcgatgtgc aggcgcgggg cgcgtatccg
    44821 gcttactctg ctcgcgtatt ccgcgaaaaa ggggtaacca tcgacaaagc accgggcgat
    44881 gatgagatcc tgaaaaacac cgtcgatttt gtctctttca gctattacgc ctcgcgctgc
    44941 gcctcggcgg agatgaacac caacaatagc agtgcggcga acgtagtgaa atcgctgcgt
    45001 aacccgtatc tacaggtgag cgactggggc tggggaattg atccactcgg tctgcgtatc
    45061 accatgaaca tgatgtacga ccgctatcag aagccgctgt ttctggtgga aaacggcctc
    45121 ggcgcaaaag atgaacttgc tgccaatggc gagattaacg atgactatcg catcagctat
    45181 ttacgcgaac atatccgcgc aatgggcgaa gcgattgcag atggcattcc gctgatgggc
    45241 tacaccacat ggggctgtat tgatttagtt tccgcctcta cgggtgaaat gagcaaacgc
    45301 tacggttttg tctttgttga ccgtgacgac gcaggcaacg gcacgctgac gcgcacacgt
    45361 aagaaatcgt tctggtggta taaaaaagtg attgccagta atggggaaga tttagagtag
    45421 gtaacctact gtcgcactaa ccaggaaggc ccgataagcg ttgcgcatcg ggcaatctgg
    45481 ggtttgctat cagttctgat tccctcatca agaacatccc tgtcctgatt acttattgaa
    45541 aaactactct tcttccaccg ctaactgcgc gaagccgccg ttcccttccc agccttccag
    45601 tcgttgataa acggtttcta ccgcatcttt aatcggctgg gtcatcgggt agtaaaagcc
    45661 gacgatatcc ggctggatgc cgaggaaaat cacttcgcca atatcttctt tcaactgatc
    45721 gataaggtag ttcaacggca tgttatgggt agtcatcata aacatctcgg cgatatcatc
    45781 cgggtcgatg atgcggattt cgccggggtt tagccccata tccgtggcgt cgacaatcag
    45841 cagccgtgtc gggcgcagtt cgcggatagc gacaatgtcg ttttccggtg cgctaccgcc
    45901 gtcaatcacc acccagttac ctttcggcgc ggcggcgcac ttttccgcca gcagcggacc
    45961 tgcgccatca tcacccatca tgctattgcc aacacagagt aaaacgtcag tcacgcagtc
    46021 tcctcaccat caaatagatg gcgttttcct gatgaatatc atgcagcata cttaacagca
    46081 ttgtactcca ttcctgctgc tgcgaagttt gcgctgcccg ggctttatcg aatgcattgg
    46141 ccagcatcgg cacatggtta atgtcgatga cgatctcacc atatttcggc acaccttcca
    46201 ttttgcggcg ggcttcgctg cccgcctcca gagtggcaat ccatgccaga tattcatccc
    46261 acgggcaggt gagcgccgct tccaggcaat cgataacccc aaggtggtga ccaatcgcca
    46321 ggctgtaata gaccacctgc tgcgcctcgg cgggcgtggc atcgttctca tcaataaatt
    46381 tacggctcag ttgactgaac accacctttt cactcatcgg atacgcgcct cttcaacaac
    46441 atgattcaga tggctgacaa tctcgttcag acgcggatcg ttttccgctt ccagccagcg
    46501 tgcaacctgt tcttcgccct gccctaactg tgtaaggtaa tcatcggcaa tctgacgacc
    46561 gtaacgataa cccgccagtc gacgtgcttc gcgatccact ttcacacgca gcggctgcac
    46621 catatcgcca tgcaggatct ccgccggttg ttcatcctgc tcacccggcc cacgggcgtg
    46681 aattttctgc tccagcaggc cgagcgccat tgcaaagccg tacagcgtgg cggctggcgt
    46741 tggcgggcag ccagggatat aaacatccac cgggacgatt ttatccgtac cgccccacac
    46801 gcagtagaga tcgtggaaga tcccgccgct gttaccgcag gcaccgtagg agatacaaat
    46861 tttcgggtcc ggcgcggact gccacgcacg cagcgcaggg gatcgcattg cacgggtgac
    46921 cgcgccggta aacagtaaaa tatccgcatg acgcggtgaa ggaacgactt taatgccgaa
    46981 gcgttctgca tcgaacagcg gcgacaaagt ggcgaaaatt tcgatttcgc agccgttgca
    47041 gccgccgcag tccacgcggt aaacgtaggc agaacgtttg atttttttca gtaacgacgc
    47101 cttcatgctg gcgatggatt catccaccgt catcgggacc ggaatgccgt tggcgtcacg
    47161 ggggcctaat aaattgctca tcagatggcc tctttcatat ggcgagtcag ttcaatacgg
    47221 tcggacggca ccaggcattt ctggcgctta cattccgggc aagtctcaaa gctttcgcgg
    47281 tggttttccg cgcggctgtc gccattgtgc ttaagcagcg caatggcgta gtcgatctct
    47341 ttctggacgg cgaaaggacg attgcagacg cggcagttgc acagcgcgaa gcgggactgt
    47401 tgcaggaagt cttctttctt ccacaccgcc agttcgtact cttgcgacag tttgattgcc
    47461 gccgtcgggc agacttcttc gcagcgtccg cagaagatgc agcgcccgag attgaactcc
    47521 cacgccagct caccggtggc gaggtcagtt tcaaccgtta aggcgtttga cgggcaggca
    47581 ttgacgcagg ccgcgcagcc gatgcactgc tgcgggttct gctctggctt accacggaag
    47641 tttttatcaa ccgcaatcgg ttccagcgga taagacgagg tcgccgtgcc ggttttgatg
    47701 acttttttga taaaggtaaa catggcgatt ccttatttca gcggcgagtt tttacgctca
    47761 atgctgtaac gctcgagttc tttgtacggc accactttgc tctttttctt acgcacatcg
    47821 accacggtca tgcggtcggt acaggagtag caagggtcca gactaccgat aatcagcggc
    47881 gcatcagaaa cggtgttgcc gcgcagcatg tagcgcaggg tcggccagtt cgcgtaggtc
    47941 gcggcacggc agcgccagcg gtacagcttc tggttgtcgc cggtcatgct ccagtggata
    48001 tcatcgccgc gcggcgcttc ggcaaagccc agcgcaaaac ggtgcggaat gtaggtaaag
    48061 ccttccacca tcagcgggcc gcccggcagg ttatccagac cgtagtcgat catgttcagc
    48121 gcggtataga cttcgttaat acgcactttc agacgcgaaa taacgtcgca gccctgctcg
    48181 ctgtggactt ccattggcag caggccataa ccaacaaacg ggtgatcggc gcgggtatcg
    48241 cgggcgtgac cgctggcacg gaccatcggg ccgacgttac tgaagtcgcg agcgatttcc
    48301 gggtccagac gaccaatgcc gacagtgcgc tgttccatgt tcggcgtact cagcagcaca
    48361 tccaccagct cctgcacttc acgacgcatc tgttgcgcca gttgacgggt ctggatcatg
    48421 tcatctttca gcagatcgcg acgaatcccg ccgatcaggt tcaggccgta ggttttacgc
    48481 gcaccggtaa ggatctctgc cattttcatg gaggtttcac gcacgcggaa gaactgcata
    48541 aagccggagt caaagccggt aaagtgacag gcgaggccga ggttgagcag atgcgagtgc
    48601 agacgttcta cctccagcag aatggcgcgg atcatctgcg cacgttctgg cacctgaata
    48661 cccatcgcgt tttccaccga cgtggtgtag gcggtgctgt gagcaaaacc gcagatcccg
    48721 cacacacggt cagagaggaa ggtcacttcg ttataaccca tgcgggtttc tgccagtttt
    48781 tccatgccgc gatggacata gaacaggcgg tagtcggcgt cgataatgtt ttcgccatcg
    48841 acgaacagac ggaagtggcc cggttcgtca gaagtgacgt gcagcggacc aatcggcacg
    48901 acgttgtttt tcttgtcgcc cagttcgttg atgaactcgt aggtttcagc atcggtggtc
    48961 ggtgccggac gctgacgata atccatgctg tctttacgca gcggataaag ttcatccggc
    49021 cagtcatccg gcagcaccag acgacgttca tccggcaaac caaccggaat caaaccgtac
    49081 atatcacgta cttcacgctc gccccacacc gcagccggaa cgcgcggcgt cacggaagga
    49141 tactccggtt tgttggcatc aacttcgacg cgaaccgtta cccagcactt agtccccttc
    49201 tccatcgaca gcacgtagta aacggcgtaa tgaccattca gtttgcgttc gtcgttacca
    49261 aaaagcaccg acagccagcc cccctgcttg tagtagagaa actccaccac ttccggcaga
    49321 tagttcacct ttacggtgac agtcagctga tctttggtct gccaggcgtg gtccagcacg
    49381 acgcccggaa atgcctcatt cagcgcggcg agataatgtt gacctaattt ttcttcagac
    49441 atgctcaaac tctctttaat cacgccgcca gcaaggagac gaacgctaaa aatgcaaagc
    49501 caaacccagc ccaggtaatg cgcggagtaa tatcaagacg cagacgggcc atgctgtttt
    49561 cgaacagcgc gataaccagg acgccgacca ccagtttgac gatggcaatc accaacgcca
    49621 gcagcagccc accgacggtg aaggtttcca tttgtcccca cggaataaac accccgacga
    49681 acatctgcag caccaccaac tgtttcaggc tgatacccca tttcatgacg ccaaagccgc
    49741 tgccgctgta ctcagagagc ggaccttcct gcaactcctg ctcggcttcc gccaggtcga
    49801 acggcagttt gcccatttcg ataaaagtgg cgaacgcaca ggcacaaagc gccagcacca
    49861 gcgggatgct ctggctcagc ggccagtgat aaacggtgtc ggtgatgttg ctgatgttgg
    49921 tggaactggc aacctgtgcg gcaacccaca gaccaagcag cagcatcggt tcgaccagca
    49981 cgccaagcat cgcttcacgg ctcgcgccga tagcggtaaa cggactaccg gtatccagac
    50041 cagaaatggc aaagaagaaa cgcgcgatgg caaagagata cagtaaggtg atcaaatcac
    50101 ccagttgcgg cagcggagaa ccgacggtca ccaccggcag cgcagtagcg atagtcagca
    50161 tgacgcccac catcacatac ggcgtcaggc ggaacaccca gccggaggca tccgggccaa
    50221 cgctctggcg acccagcagt ttgataatgt cgcgatactc ctgcaacacg cccggcccgc
    50281 gacggttatg caagcgggcg cgcgccacgc gggtaatacc ggagagcagc ggcgcaacgg
    50341 caaatagcac cagcgcctga attaacggat ataaaacact cattctcagg ctcctcgtga
    50401 aacaataatc accaccagca ccgccagttc aaccagcgcc atccggcgga acagcaacgc
    50461 actgccttcg cactgccagc ccggcaccag cgacaccgga ttcagccatt tgcgcagttt
    50521 cagcaccggc gcaaacgcct gtttcaccgg catggcaaaa ccgtgggcgg taattaccat
    50581 tgatttttca tggtcgtagc cgcacaccca ggccgcgccg cgggaacgcg atggcaaacg
    50641 atcgcctttg caaatcgcca taatgatgaa tggcagcagc gggcaggcaa tcagcagcaa
    50701 cgtgatcatc ggttgagaaa cggtggtgtt agcaggttcc agcggcagag gtacagcagc
    50761 agagagcatc ggcagcagcc acggcgcagc aacaccgcca attacgcagc aaatcgccag
    50821 tgccactacg cttacgctca tcaggagcgg tgcacaggtg gcgttttcgg cttctttggt
    50881 acgcggcgcg ccgaggaacg tgacgccata gactttcgcc atacacatca ccgccagcgc
    50941 accggtaatt gccagaccca cagcgagcag cggccccagc agacgggcaa caaacgcgcc
    51001 actattgctc agtttgaaga aggattgata gataacccat tccccggcaa aaccgttcag
    51061 cggcggcagt gcagccattg ccatcagccc gactaacata gcgatggaga taaccggcat
    51121 tttcttgcca ataccaccga gtttttcgat atcgcgatga ccggtacgga accagacgct
    51181 ccccgccccg aggaacagta cgcttttgaa caggctatgg ttaagcagat ggtacagacc
    51241 accgaccagg ccaagagcaa tcagcgccgg ttgttcgagc gcgatacccg ttacgccagc
    51301 gcccagcccc agcaggataa tgccgatatt ttccagggtg tggtaagcca gtagacgctg
    51361 gatattatgt tccatcaacg catacagacc gccgacaaac gcggtgatca taccgagcac
    51421 cagcagcgcg atcccccacc acagcggtgc attaccgccc agcagtgaca gggttaaaat
    51481 gcccagcagg ccaattttca tgactaccgt agaaaacaac gcggcagctg gcgcagaggc
    51541 gttcgcatgt gcctgcggca cccagccgtg cagcggaata atcccggcca gcaggccaaa
    51601 gccaatcaca ctgagcagcc agatatcgga accgagcggc agctgttgca tacgcatatc
    51661 cagcaggcgc agatccagcg tgccgtaacg ctgccacagc agccagcaag caatcgccag
    51721 cagcagagtg ccaagacgcc ccagcgcaaa ccacagtttg ccctctttgc tgttgctggt
    51781 gaggaacacc gcacacaagg ccatgacttc ggccattacc acgaacatgc cgaggttgct
    51841 ggcaatgacg gcgcagacgg cggcagccat caacatattg atctgcaagc cgttgcactt
    51901 cacctgcgcg tggcgatgcc agtcaatgtt gtagaggctg acaaacagac cgcacagacc
    51961 gagcgtaatc agccaaatcg cgttaagcgg agagatttgc acatcgtagc ttaccagcga
    52021 cagcgcaccg ctcacgccaa ccgcgccagt cagtacagtg aagcccgcgg ctgccgtata
    52081 cagactacca accgcgccgc caattccggc tatccagccg cttagcgcct tttgaaaaga
    52141 aaagagaaat gccagaacag cggcggcgac aaaccacgcc acgccgctat tgatcaggga
    52201 aattgcgctc atttagcctc tccactttga gcctgctgaa acaaggtgag atcgccaaag
    52261 tccgtgttaa aggtcagctc acgcttacgt ttgctgacgc gggcgatatc ggtgttatcc
    52321 accagatgca gggctttagt cgggcacatc cgcacgcagg ccggaccttg ttcatcaaag
    52381 ctacaaaggt cacatttgac ggcgatcgcg cgaatacccg gcacccagtc aagcagcgta
    52441 ctgatacgcg ccggagcagg cggtgccggt ggcgctttcg gggtattggc atttgcagga
    52501 atatccagcg gacggctgcc ggaaaattca attgcgccaa acgggcaggc gatgccgcac
    52561 agcttgcagc ttacgcacag gctttcattc aactgcacgg ccccatcaac gcgggtgatg
    52621 gcgttaaccg ggcagaccac cgcgcagggt gcatcttcac agtggtgaca gagctgcggc
    52681 gcagattctt tttcatttag catcactctc aggcgcggca ttgattgcag gccgtgctgg
    52741 cgatgcgtct ctgaacaggc ggcctcacaa gtgtggcaac cgatacagag cgtggagtca
    52801 gcaattacaa aacgattcac caggcattcc tcaggtgatt gtcatttttg acgaaaacat
    52861 gccgatgaaa tgtcattttc gacactcatc gacacgccca tccccaaaca ggcgtaacgc
    52921 ctgcaaaacg ggcaaagcct cagctcatgc tgccgggctt tgtcccttta ccagttggct
    52981 taaattcacc ggcacattgt tttcaatagc ggtgtataag ctgtcgtaaa cgccagcgat
    53041 tttttcgaga tagtgttcca gaacctgttc gcgatcgcgg atgttgacct tgccatcaat
    53101 catgccgtca atgctcagtt gcgcctgcgc aatccgctgt ttgtcttcgc catcttttgt
    53161 ttcgacgtaa tactcgatgg tgtggctgtt aaagccatcc gccagggtga cgtaaatacg
    53221 aaaatgttca aaaaggacga aacagagttc tttgtccggc gcgcagctca tggcgtgatg
    53281 caggcgcgct ttcgataacg tgatcccctg aaccagcgaa ttgcagtaga tgtgccactg
    53341 gtcctgtagg cgacgatgcc gctgtgcgat gtaatcggct ttctcgctta tttcccaaat
    53401 agtcattgtc aggttacccg tttaacagag atgccagctt taagcatttt ttgtgccaac
    53461 ttttaattta ttattattaa agcgattttt aagattttgg tgaatttcgt cgccgtgtcg
    53521 acgtgtcatt tcgacatcat cgacattatt cactgcaggg ataatcaaca ctggcacaat
    53581 tattgcttgt agctggcaat agttaatggg aggcgatatg cacgaaataa ccctctgcca
    53641 acgggcactg gaattgatcg aacagcaggc cgcaaaacac ggcgcaaaac gcgtaactgg
    53701 ggtctggctc aaaattggcg cattttcttg tgtcgaaacc agctctcttg ccttttgttt
    53761 tgatctggtt tgccgcggca gcgtggcgga aggttgtaaa ctgcacctcg aagaacaaga
    53821 ggccgaatgc tggtgtgaaa catgccaaca gtatgtgacg ctactgaccc agcgcgtccg
    53881 ccgctgtcca cagtgtcatg gtgacatgct acagattgtg gcagacgacg gtttacagat
    53941 tcggcggata gaaatagacc aggagtgagc gatgtgtaca acatgcggtt gcggtgaagg
    54001 caacctgtat atcgagggtg atgaacataa ccctcattcc gcgtttcgta gcgcgccatt
    54061 tgccccggcg gcacgcccga agatgaaaat caccggcatt aaagcgcctg aatttacccc
    54121 cagccagact gaagaaggcg acctgcatta cggtcatggc gaagcgggca ctcacgcacc
    54181 gggcatgagc cagcgtcgga tgctggaagt cgaaattgac gtgctggaca aaaataaccg
    54241 tctggctgaa cgcaaccgcg cgcgctttgc tgcctgcaag caactggtgc tcaacctggt
    54301 ttccagccca ggttccggta aaaccaccct gctgacggaa accttaatgc gcctgaaaga
    54361 cagcgttccg tgcgcggtta ttgaaggtga ccagcaaacc gtgaacgatg ccgcacgcat
    54421 tcgcgctacc ggcacaccag cgattcaggt gaacaccggt aaaggctgcc atcttgacgc
    54481 acagatgatt gccgacgccg caccgcgtct gccactggac gataacggta ttctgtttat
    54541 cgaaaacgtt ggcaacctcg tatgcccggc cagcttcgat ctcggtgaaa aacacaaagt
    54601 ggcggtgctt tccgttaccg aaggtgaaga caaaccgctg aaatatccgc atatgtttgc
    54661 cgccgcctcg ctgatgctgc tcaacaaagt tgacctgttg ccgtatctca actttgacgt
    54721 tgagaagtgc atcgcctgcg cccgcgaagt caatccagaa attgaaatca tccttatttc
    54781 cgccaccagc ggcgaaggga tggaccagtg gctgaactgg ctggagacac agcgatgtgc
    54841 ataggcgttc ccggccagat ccgcaccatt gacggtaacc aggcgaaagt cgacgtctgc
    54901 ggcattcagc gcgatgtcga tttaacgtta gtcggcagct gcgatgaaaa cggtcagccg
    54961 cgcgtgggcc agtgggtact ggtacacgtt ggctttgcca tgagcgtaat taatgaagcc
    55021 gaagcacgcg acactctcga cgccttgcaa aacatgtttg acgttgagcc ggatgtcggc
    55081 gcgctgttgt atggcgagga aaaataatgc gttttgttga tgaatatcgc gcgccggaac
    55141 aggtgatgca gttaattgag catctgcgcg aacgtgcttc acatctctct tacaccgccg
    55201 aacgccctct gcggattatg gaagtgtgcg gtggtcatac ccacgccatt tttaaattcg
    55261 gcctcgacca gttactgcct gaaaacgttg agtttatcca cggtccgggt tgcccggtgt
    55321 gcgtactgcc gatgggcaga atcgacacct gcgtggagat tgccagccat ccggcagtca
    55381 tcttctgtac ctttggcgac gccatgcgcg tgccggggaa acagggatcg ctgttgcagg
    55441 caaaagcacg cggtgccgat gtgcgcatcg tctattcgcc gatggatgcg ttgaaactgg
    55501 cgcaggagaa tccaacccgc aaagtggtgt tcttcggctt aggttttgaa accaccatgc
    55561 cgaccaccgc catcactctg caacaggcga aagcgcgtga tgtgcagaat ttttacttct
    55621 tctgccagca tattacgctt atcccgacgc tgcgcagttt gctggaacag ccggataacg
    55681 gtatcgacgc gttcctcgcg ccgggccacg ttagtatggt tatcggcact gatgcctata
    55741 attttatcgc cagcgatttt cagcgtccgc tggtggtggc tggtttcgaa ccccttgatc
    55801 tactgcaagg cgtggtcatg ctggtggagc agaaaatagc ggcccacagc aaggtagaga
    55861 atcagtatcg tcgggtggta ccggatgccg gtaacctgct ggcgcaacag gcgattgccg
    55921 atgtgttctg tgtcaacggc gacagcgaat ggcgcggctt aggcgtgatt gaatcttctg
    55981 gcgtgcacct gacgccggat tatcaacgat tcgatgccga agcacatttc cgcccggcac
    56041 cgcagcaggt ctgcgatgac ccgcgcgcgc gttgtggcga agtcttgacg ggcaaatgta
    56101 agccgcatca atgcccgctg tttggtaaca cctgtaatcc tcaaaccgcg tttggtgcgc
    56161 tgatggtttc ctccgaagga gcgtgcgccg cgtggtatca gtatcgtcag caggagagtg
    56221 aagcgtgaat aatatccaac tcgcccacgg tagcggcggc caggcgatgc agcaattaat
    56281 caacagcctg tttatggaag cctttgccaa cccgtggctg gcagagcagg aagatcaggc
    56341 acgtcttgat ctggcgcagc tggtagcgga aggcgaccgt ctggcgttct ccaccgacag
    56401 ttacgttatt gacccgctgt tcttccctgg cggtaatatc ggcaagctgg cgatttgcgg
    56461 caccgcgaat gacgttgcgg tcagtggcgc tattccgcgc tatctctcct gtggctttat
    56521 cctcgaagaa ggattgccga tggagacact gaaagccgta gtgaccagca tggcagaaac
    56581 cgcccgcacg gcaggcattg ccatcgttac tggcgatact aaagtggtgc agcgcggcgc
    56641 ggcagataaa ctgtttatca acaccgcggg catgggcgca attccgacga atattcactg
    56701 gggcgcacag acgctaaccg caggcgatat attgctggtt agcggtacac tcggcgacca
    56761 cggggcgact atccttaacc tgcgtgagca gctggggctg gatggcgaac tggtcagcga
    56821 ctgcgcggtg ctgacgccgc ttattcagac gctgcgtgac attcccggcg tgaaagcgct
    56881 gcgtgatgcc acccgtggtg gtgtaaacgc ggtggttcat gagttcgcgg cagcctgcgg
    56941 ttgcggtatt gaaatttctg aatcagcgct gccggttaaa cctgccgtgc gcggcgtttg
    57001 cgaattgctg ggactggacg ccctgaactt tgccaacgaa ggcaaactgg tgatcgccgt
    57061 tgaacgcaac gcggcagagc aagtgctggc agcgttacat tcccatccac tggggaaaga
    57121 cgcggcgctg attggtgaag tggtggaacg taaaggtgtt cgtcttgccg gtctgtatgg
    57181 cgtgaaacga accctcgatt taccacacgc cgaaccgctt ccgcgtatat gctaataaaa
    57241 ttctaaatct cctatagtta gtcaatgacc ttttgcaccg ctttgcggtg ctttcctgga
    57301 agaacaaaat gtcatataca ccgatgagtg atctcggaca gcaagggttg ttcgacatca
    57361 ctcggacact attgcagcag cccgatctgg cctcgctgtg tgaggctctt tcgcaactgg
    57421 taaagcgttc tgcgctcgcc gacaacgcgg ctattgtgtt gtggcaagcg cagactcaac
    57481 gtgcgtctta ttacgcatcg cgtgaaaaag acacccccat taaatatgaa gacgaaactg
    57541 ttctggcaca cggtccggta cgcagcattt tgtcgcgccc tgatacgttg cattgcagtt
    57601 acgaagaatt ttgtgaaacc tggccgcagc tggccgcagg tgggctatac ccaaaatttg
    57661 gtcactattg cctgatgcca ctggcggcgg aagggcatat ttttggtggc tgtgaattta
    57721 ttcgttatga cgatcgcccc tggagcgaaa aagagttcaa tcgtctgcaa acatttacgc
    57781 agatcgtttc tgtcgtcacc gaacaaatcc agagtcgcgt cgttaacaat gtcgactatg
    57841 agttgttatg ccgggaacgc gataacttcc gcatcctggt cgccatcacc aacgcggtgc
    57901 tttcccgcct ggatatggac gaactggtca gcgaagtcgc caaagaaatc cattactatt
    57961 tcgatattga cgatatcagt atcgtcttac gcagccaccg taaaaacaaa ctcaacatct
    58021 actccactca ctatcttgat aaacagcatc ccgcccacga acagagcgaa gtcgatgaag
    58081 ccggaaccct caccgaacgc gtgttcaaaa gtaaagagat gctgctgatt aatctccacg
    58141 agcgggatga tttagccccc tatgaacgca tgttgttcga tacctggggc aaccagattc
    58201 aaaccttgtg cctgttaccg ctgatgtctg gcgacaccat gctgggcgtg ctgaaactgg
    58261 cgcaatgtga agagaaagtg tttaccacta ccaatctgaa tttactgcgc cagattgccg
    58321 aacgtgtggc aatcgctgtc gataacgccc tcgcctatca ggaaatccat cgtctgaaag
    58381 aacggctggt tgatgaaaac ctcgccctga ccgagcagct caacaatgtt gatagtgaat
    58441 ttggcgagat tattggccgc agcgaagcca tgtacagcgt gcttaaacaa gttgaaatgg
    58501 tggcgcaaag tgacagtacc gtgctgatcc tcggtgaaac tggcacgggt aaagagctga
    58561 ttgcccgtgc tatccataat ctcagtgggc gtaataatcg ccgcatggtc aaaatgaact
    58621 gcgcggcgat gcctgccgga ttgctggaga gcgatctgtt tggtcatgag cgtggggctt
    58681 ttaccggtgc cagcgcccag cgtatcggtc gttttgaact ggcggataaa agctccctgt
    58741 tcctcgacga agtgggcgat atgccactgg agttacagcc gaagttgctg cgtgtattgc
    58801 aggaacagga gtttgaacgc ctcggcagca acaaaatcat tcagacggac gtgcgtctaa
    58861 tcgccgcgac taaccgcgat ctgaaaaaaa tggtcgccga ccgtgagttc cgtagcgatc
    58921 tctattaccg cctgaacgta ttcccgattc acctgccgcc actacgcgag cgtccggaag
    58981 atattccgct gctggcgaaa gcctttacct tcaaaattgc ccgtcgtctg gggcgcaata
    59041 tcgacagcat tcctgccgag acgttgcgca ccttgagcaa tatggagtgg ccgggtaacg
    59101 tacgcgaact ggaaaacgtc attgagcgcg cggtattgct aacacgcggc aacgtgctgc
    59161 agctgtcatt gccagatatt gctttaccgg agcctgaaac gccgcctgcc gcaacggttg
    59221 tcgctcagga gggcgaagat gaatatcagt tgattgttcg cgtgctgaaa gaaactaacg
    59281 gcgtggttgc cgggcctaaa ggcgctgcgc aacgtctggg gctgaaacgc acgaccctgc
    59341 tgtcacggat gaagcgactg ggaattgata aatcggcatt gatttaactg caaattgccg
    59401 gacagatctg cctgtccggc atactattca cgaggttttt tcggacgata tttttccggc
    59461 agttctggca ccggacactt gtcatcgatg agatgacgca cggttaagat cggatgacgc
    59521 cacagcattc tcggcccggc ccaacgcata atctgtttca tctcttcacg ctttgcaggc
    59581 tggtaacagt gcaccggaca ctgcttacag gctggtttct cttcgccgaa cacacattta
    59641 tccagccgct tttgcgcgta aacaaacaac gcctcgtaat gctccggctc cgctgacgcc
    59701 tgcgggcatt tcgcttgata aagatcgatc atttttttaa tcgtcagttt ttcacgagag
    59761 atacgcttgc cggacatact gcctccacct cattaagatg catttatatt acaacttaat
    59821 cttaaagggc actatgactc taaagaagaa gggttagcca accgatacaa ttttgcgtac
    59881 ttgcttcata agcatcacgc aaaagctgca aaacagcatc tttcccggaa ccagcatcaa
    59941 gaactcgccg ttcgcttctt cccctgaaat gattaactcc ggtatcatgt gcgccttatg
    60001 tgattacaac gaaaataaaa accatcacac cccatttaat atcagggaac cggacataac
    60061 cccatgagtg caatagaaaa tttcgacgcc catacgccca tgatgcagca gtatctcaag
    60121 ctgaaagccc agcatcccga gatcctgctg ttttaccgga tgggtgattt ttatgaactg
    60181 ttttatgacg acgcaaaacg cgcgtcgcaa ctgctggata tttcactgac caaacgcggt
    60241 gcttcggcgg gagagccgat cccgatggcg gggattccct accatgcggt ggaaaactac
    60301 ctcgccaaac tggtgaatca gggcgagtcc gttgccatct gcgaacaaat tggcgatccg
    60361 gcgaccagca aaggtccggt tgagcgcaaa gttgtgcgta tcgttacgcc aggcaccatc
    60421 agcgatgaag ccctgttgca ggagcgtcag gacaacctgc tggcggctat ctggcaggac
    60481 agcaaaggtt tcggctacgc gacgctggat atcagttccg gtcgttttcg cctgagcgaa
    60541 ccggctgacc gggaaacgat ggcggcagaa ctgcaacgca ctaatcctgc ggaactgctg
    60601 tatgcagaag attttgctga aatgtcgtta attgaaggcc gtcgcggcct gcgccgtcgc
    60661 ccgctgtggg agtttgaaat cgacaccgcg cgccagcagt tgaatctgca atttgggacc
    60721 cgcgatctgg tcggttttgg cgtcgagaac gcgccgcgcg gactttgtgc tgccggttgt
    60781 ctgttgcagt atgcgaaaga tacccaacgt acgactctgc cgcatattcg ttccatcacc
    60841 atggaacgtg agcaggacag catcattatg gatgccgcga cgcgtcgtaa tctggaaatc
    60901 acccagaacc tggcgggtgg tgcggaaaat acgctggctt ctgtgctcga ctgcaccgtc
    60961 acgccgatgg gcagccgtat gctgaaacgc tggctgcata tgccagtgcg cgatacccgc
    61021 gtgttgcttg agcgccagca aactattggc gcattgcagg atttcaccgc cgagttgcag
    61081 ccggtactac gtcaggtcgg cgacctggaa cgtattctgg cgcgtctggc gttgcgtacc
    61141 gctcgcccac gcgatctggc ccgtatgcgt cacgctttcc agcaactgcc ggagctgcgt
    61201 gcgcagttag aaactgttga tagtgcacca gtacaggcgc tacgtgagaa gatgggcgag
    61261 tttgccgagc tgcgcgatct gctggagcga gcaatcatcg acacaccgcc ggtgctggta
    61321 cgcgacggtg gtgttatcgc atcaggctat aacgaagagc tggatgagtg gcgcgcgctg
    61381 gctgacggcg cgaccgatta tctggagcgt ctggaagtcc gcgagcgtga acgtaccggc
    61441 ctggacacgc taaaagttgg ctttaatgcg gtgcacggct actacattca aatcagccgt
    61501 gggcaaagcc atctggcacc tatcaactat atgcgtcgcc agacgctgaa aaacgccgag
    61561 cgctacatca ttccagagct aaaagagtac gaagataaag tcctcacttc aaaaggcaaa
    61621 gcactggctc tggaaaaaca gctttatgaa gagctgttcg acctgctgtt gccgcatctg
    61681 gaagcgttgc aacagagcgc gagcgcgctg gcggaactcg acgtgctggt gaacctggcg
    61741 gaacgggcct ataccctgaa ctacacctgc ccgaccttca ttgataaacc gggcattcgc
    61801 attaccgaag gccgccatcc ggtggttgaa caggtgctga acgagccatt tatcgccaac
    61861 ccgctgaatc tgtcaccgca gcgccggatg ttgattatta ccggtccgaa catgggcggt
    61921 aaaagtacct atatgcgcca gaccgcactg attgcgctga tggcctatat cggcagctac
    61981 gtaccggcgc aaaaagtcga gattggcccg attgaccgta tctttacccg cgtaggggca
    62041 gcggatgatc tggcttccgg gcgttcaacc tttatggtgg agatgaccga aaccgctaat
    62101 attctgcata acgccaccga gtacagtctg gtgctgatgg atgagattgg gcgcggaacg
    62161 tccacttacg atggtctgtc gctggcgtgg gcgtgcgcgg aaaatctggc gaataagatt
    62221 aaggcgttga cgctgtttgc cacccactat ttcgagctga cccagttacc ggagaaaatg
    62281 gaaggcgtcg ccaacgtgca tctcgatgca ctggagcacg gcgacaccat tgcctttatg
    62341 catagcgtgc aggatggcgc ggcgagcaaa agctacggcc tggcggttgc agctctggcc
    62401 ggcgtgccaa aagaggttat taagcgcgca cggcaaaaac tgcgtgagct ggaaagcatt
    62461 tcgccgaacg ccgccgctac gcaagtggat ggtacgcaaa tgtctttgct gtccgtaccg
    62521 gaagaaacct cgcctgcagt cgaggcactg gaaaacctcg atccggattc actcaccccg
    62581 cgtcaggcgc tggaatggat ttatcgcctg aagagtctgg tgtaataata attcccgata
    62641 gtcttttgct atcgggaata ttaacgataa ctgacgaatc aaataaaaat accctgtata
    62701 ataggaaagc ttattttaca gggtaaaacc atgccatcta cacgctatca aaaaatcaat
    62761 acccatcact atcgccatat atgggtcgtt ggtgatattc atggtgaata tcagttatta
    62821 caatcccgct tacatcaact ctcttttttc cccgaaaccg acttacttat ttctgtcggc
    62881 gataatattg atcgtgggcc ggagagtctt aacgtcctgc gcctgctaaa ccagccctgg
    62941 tttatctccg ttaaaggcaa ccacgaagca atggcgctgg atgcgttcgc gaccggcgat
    63001 ggcaatatgt ggcttgccag cggtggtgac tggtttttcg atttaaatga ttcagagcaa
    63061 aaagaagcta cagatctgtt gctgaaattc catcaccttc cacatattat tgaaatcact
    63121 aacgacaaca taaaatatgt catcgcacat gcagattatc cggggagtga atatctcttt
    63181 ggtaaagaaa tagcggagag cgaattactc tggcctgttg atcgtgtgca gaaatcgctt
    63241 aatggcgagt tacaacaaat aaacggcgct gattatttta tatttggaca tatgatgttt
    63301 gataacattc agacgttcgc taaccagatt tatattgata ccggatcgcc gaaaagcggg
    63361 cggctgtcat tttataaaat aaagtaatga ggccgggtaa agcaaagcca ctacccggca
    63421 ctttttatta gcgcttacct tccgccagca gcggcgggat gctcggcacc attggcgcgt
    63481 catcaatatc tttctgcgtc atgcggaacg cttcgggata atgttcgcgg ctggtacggc
    63541 gcagcggttc ggtatcgcgc caggtataaa ggcaatgctg gcactgatat accgtccaga
    63601 catctttcac cggcgatttc gccatcactt caatctgttc atcggcacaa cgtggacaaa
    63661 tcatcttgtt ctccttattt acgtgcggcc agcatagcgg tcagtttttc agcccaggct
    63721 ttggtttccg gcaaatccac caccggctgg ctgtagtgac cacggttgtc cggggcgaca
    63781 ggcgtggtgg cgtcgataat cagcttgtcg gtgatccccg ccgggcttga gccagggtcg
    63841 agttccagca cggacatatt cggcaactgc accaaatccc ctgccggatt tactttcgag
    63901 gagagcgccc acatcacctg cggcaggttg aacgggtcaa cgtcttcatc gaccataatc
    63961 accatcttta cgtagcccag accgtgcggc gtggtcatcg cacgcaggcc caccgcgcgg
    64021 gcaaagccgc cgtagcgttt tttggtggag ataatcgcca gcaggccgtg ggtgtacatg
    64081 gcgtttaccg cctgcacttc cgggaactcg gctttcagtt gttgatacag cggcacacag
    64141 gtggctggcc ccatcaggta gtcgatttcg gtccacggca tgccgaggta cagcgattcg
    64201 aaaatcggcc tggtgcggta agagacttta tcgatacgca ccacggtcat gttacgcccg
    64261 ccggagtagt gcccggtaaa ctcaccgaac ggcccttcga tttcgcgttt gcggctttcg
    64321 ataacccctt cgaggatcac ttctgaaccc cacggcacat caaaaccagt caatggcgcg
    64381 gtggcgatcg ggtacgggct ttcgcgcagc gcgcctgcca tttcatactc agactgatcg
    64441 tatttcagcg gcgtggcgcc cataagggtg atgatcggat cgttacccag ggtgatggca
    64501 atcggcagat cttcaccgcg ctcttccgct ttatgcagat gcagggcgat atcgtgcatc
    64561 ggcaccggtt gcaggccgag cttacgcttg cccttcactt ccatgcggta gataccgacg
    64621 ttctgcttgc cgaagttatc cgggtcgagc ggatcgcggg aaaccacgca cgctttgtcg
    64681 agatagaaac cgccatcacc gtcgtttaaa cgaaacagcg gcaggatatc gaacagatta
    64741 atctcgtcac catcgacggt gttctgcgcc caggctggat tggcgcggcg ctccggggcg
    64801 atcgggaagt tatcccagcg gcggataaac tcatcaatct gttttttaac cggggtgttt
    64861 ggcggcaggc ccaaggaaat cgcgtggttc tgccaggaac cgatggtatt catcgccacg
    64921 cgggcatcgg taaaaccgcg aatattatca aaccacagcg ccggtgcacc atcgccgata
    64981 cgcccggtgg cgttggcagc ggcagccaga tccggttccg cgttcacctc ttcgctgatt
    65041 ttcagtaact ggccgtggtc atcaagcgcc tgtaaaaagc tgcgtaaatc atcaaatgcc
    65101 attattcatt ctcctgagaa aaattccggg cctgcggcaa tccttgccag cgcctggcgt
    65161 agggatgttc aaggccaaat tgatccagca cgcgggctac cacgtggtgg acaatgtcat
    65221 ctaccgtttc gggatggtta taaaaggcag gcatcggcgg caccatcgcc acgcccatgc
    65281 gtgaaagtgc gagcatattt tcgagatgga tggtgctaag cggcatttca cgcggcacca
    65341 gcaccagttt gcggccttct ttaagcacga cgtccgccgc gcgccctacc aggccatcag
    65401 cgtaaccagc gcggataccg gcgagcgttt tcatactgca cggaataacg atcatgccgt
    65461 ctgtacgaaa ggaacctgag gagatggtcg ccgcctgatc cgccgggtta tggctgaagt
    65521 cagcgagggc agcaacatcg cgggcgctgt aaggcgtttc cagttcaatg gtggttttcg
    65581 cccacttcga catcaccaga tgagtctcga cattcggcat ctcccgcagc gcttgcagta
    65641 atgccacacc aagaggcgca ccggtagccc ctgtcatccc gacgatcagt ttcattgctt
    65701 actctcttaa ttgttcgtat acgaacatta ttattaaaat acgcctctgt tctcattgcg
    65761 gcaagcactg aaaagtaaca acatcccctt cgtcactgaa aaaccgctat gatagcggca
    65821 gatagtttgc ccggagttta catgacgtta cgaaataaag cgttccatca gttacgacag
    65881 ctttttcagc agcacacggc tcgctggcag cacgagttac ctgacctgac caaaccacag
    65941 tatgcggtga tgcgcgccat tgctgataag cctggtatcg aacaggtggc actgatagaa
    66001 gcagcagtca gcaccaaagc aacgttggca gaaatgttgg caagaatgga gaatcgtggc
    66061 ttagtcagac gtgaacatga tgccgctgac aagcggcgtc gctttgtctg gctgactgcc
    66121 gaaggagaaa agatacttgc tgcggcgata ccgattggtg acagcgtgga tgaggaattt
    66181 ttggggcgtt tgagtgcaga agagcaggag ctgtttgtgc agctggtgcg caagatgatg
    66241 aacacatagg atgcaaattg ccgggtagga cgctgacgtg tcttatccag gcgacaaaaa
    66301 cacaagcttc ccaaacaaaa gaaaaaggcc agcctcgctt gagactggcc tttctgacag
    66361 atgcttactt actcgcggaa cagcgcttcg atattcagcc cctgcgtttg caggatttcg
    66421 cgcaaacggc gcaggccttc aacctgaatc tggcgaacac gttcacgggt gaggccaatt
    66481 tcacgaccta catcttccag tgttgccgct tcgtacccca gcaaaccgaa tcgacgtgcc
    66541 agtacttcac gctgtttggc gttcagctcg aacagccatt tgacgatgct ctgcttcata
    66601 tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt catcggccag gatgtccagc
    66661 aacgcttttt cggaatcacc acccagcggg gtgtctaccg aggtaatgcg ctcgttaaga
    66721 cgaagcatac ggctgacgtc atcaactggc ttatccagtt gctctgcgat ctcttccgca
    66781 cttggttcat ggtccagctt atgggacaac tcacgtgctg ttcgcaggta aacgttcagc
    66841 tcctttacga tgtgaatcgg caaacgaata gtacgggttt ggttcataat cgcccgttca
    66901 atcgtctggc gaatccacca ggttgcgtat gttgagaagc ggaaaccacg ttccgggtca
    66961 aacttctcta ccgcacggat cagccccagg ttgccctctt cgataaggtc cagcaacgcc
    67021 agaccacgat tgccataacg gcgggcaatt tttaccacca gacgcaagtt actctcgatc
    67081 atccggcggc gagaggcgac atctccacgc agtgcgcgac gcgcaaaata aacttcttct
    67141 tcggccgtta acagtggtga ataaccaatc tcaccaaggt aaagctgagt cgcgtccaac
    67201 acacgctgtg tggctccctg cgataacagt tcctcttcgg ccaaatcgtt atcactgggt
    67261 tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc cgttctcatc aaattccgca
    67321 tcttcattta aatcatgaac tttcagcgta ttctgactca taaggtggct cctacccgtg
    67381 atcccttgac ggaacattca agcaaaagcc tggttccgcc gatttatcgc tgcggcaaat
    67441 aacgcagcgg gtttacggat ttccccttgt aacgaatttc aaaatgcaag cgtgttgaac
    67501 tggttccggt gctacccatg gttgctattt tttgccccgc cttcacttct tgttgttccc
    67561 ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta atcatcatta tgtttgatga
    67621 taatcagatt accgtagccg cgcagcgcgt taccggcata aacaacacgg ccatctgcgg
    67681 tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat ccccttgttg cccccctcag
    67741 aagcgccaaa ggtttcgatc actttgccct cagtcggcca gcgccaggtg gagataggcg
    67801 tactggttga tgtactgctg acagtcggct cggttgtgct tgctgttggt accgttacag
    67861 gcgctgtgac cgtggtcgca gttggcttgt tgttcggcaa cattttgtta gcactctgtt
    67921 cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc aacagcaacg gtggaatttt
    67981 gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc ctgggtaatg gcatttccgc
    68041 cagtgattgg cgtaccggaa gcattaccca cctgtaaggt ctgaccgacg ttcagcgcgt
    68101 atggtgcctg aatattgttg cgctgagcaa ggtcacggaa atcgttgcca gtaatccagg
    68161 cgatatagaa aagcgtgtcg ccttttttca cggtataggt actgccgcta taactgcctt
    68221 tcggaatgtt cccatactga cggttataga cgatgcgtcc gttttccatc tgtaccggct
    68281 gctgagctac tggctgcact ggctggattt gcggttgttg agtagcctga atttgtggct
    68341 gctgcaccgg ctgaatttgc ggttgctgcg ctgtagacgt cgtccccatt ttcggcggcg
    68401 gcgtaatcaa cataccagaa ttggtatgtg caggcgcatt gccattaacg gagctgaccg
    68461 gtgccggtgg atttgaagtg tcagaacagc ctgccagcca tagcgaaacc agtgacaaag
    68521 ccgcaatgcg gcgaacggtg aattttgggc ttcccgcgct catttatccc ccaggaaaaa
    68581 ttggttaata accagtgaca taattaccgt gcaaggcacc atactgaaca ctggaaaaga
    68641 tgttcacgat acgctgacct gcggcaaaat aaccaggaaa aatccaggta tttcctcacg
    68701 ttttaagcca gctcaccctt cactaaaggg acaaagcgca cggcctccac ggtatcgata
    68761 ataaattcgc ctccccgacg acgcacccgt ttcaaatact ggtgctcctc ccctacgggt
    68821 aagacgagaa tcccgccttc gtccagctgc gtcattagcg cagttggaat ttccggcggt
    68881 gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct gccaaccttg ccatccatcg
    68941 ccatgacggg ttgaaacatt atgtaaatca agatttttca ggcggcgacg cgcctgccac
    69001 tgcaagcctt taatccgttc aaccgagcaa acatgctgga caagatgcgc caggattgcc
    69061 gtttgatatc ccgaaccggt gccaatttcc agcacccgcg actgcggcgt cagctcgagt
    69121 aattcggtca ttcgcgccac catatacggc tgcgaaatcg tctgcccctg acctatcggc
    69181 aaggcgatat tgtcccaggc tttttgttca aacgcttcat caacgaattt ttcacgcggc
    69241 acggcggcaa gtgcattcag cacctgctca tcctgaatac cttgcgcacg taattgatcc
    69301 agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac tcccacgctg tttaaccagt
    69361 ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac atgcagcggc gtgatggaga
    69421 catagccctc atctaccgca gcaaaatcgg tccccggacc ggcatcacat ttaccgcccg
    69481 gcgggccaat ccagtacagc gtattgccgc gcggatcttg ctgcgggatc acctgatctg
    69541 ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt gatttgatcc aagggtaaat
    69601 ccggaacatt aatattaaga atacgcccgg tgcgcagcgg ctctttacac agtgcgcgca
    69661 aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt atgcccgtca agcgagacgg
    69721 caagcgccgg aaaacctaaa tgacggcctt ccatcgcggc ggctaccgta ccggaataaa
    69781 taacatcatc ccccagattc ggcccggcgt taattccgga cacaacaatg tccgggcgcg
    69841 gacgcatcag agcattcacg ccaagataga cgcaatcggt cggggttccc atttgcacag
    69901 caatatcacc attttcaaag gtaaacgtgc gcagggagga ttccagtgtc agagaatttg
    69961 aagcgccgct gcggttacga tcgggggcga ccacctgaac gtcagcaaac tcacgcaagg
    70021 ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc atcattactc agcaatatgc
    70081 gcataatcac ctgttgtgtt gataagttcc ctgacaacgc tggttgcaaa actacccgcc
    70141 ggaagccaga aacggatttc tacggtgacg tcatcccacc aattccagct taattgttgc
    70201 ggatacagca gcatcgctct gcgcgcggct tcaacttttt cgcgcaccag taaagcttgt
    70261 aattcagttt ctgcggcgac agctgcctgt tcgaatgcca gcgcttcacg ctgagttccc
    70321 cattcaccac tgcccggcaa tgcggcggtt atcatcagct ctttatcgtt gacgcgacgc
    70381 tgtaattcca ccagttcttc ggtggttgca acaaaccagc taccacgtcc ggctaattgt
    70441 agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt tgaggcgctc agcaacaatc
    70501 tgattaaaca acgcactgcg ggctgccgac aaccaaaaac tccgtttatt gcgatcgcgc
    70561 accggagtat tggtttgcgc ccagcgcagc gcgccctgca agttgctacc gccaatccca
    70621 aaacgttggg caccgaagta gttcggtaca ccttttacgc aaatatcgat cagacgttgt
    70681 tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg taaaggcgtt acctttcagc
    70741 gcccctaaac gcagcttgcg cttgtgccgc gcatactcca gcacctggca gccttccaat
    70801 tgaaaggcgc tcagatcggg catttccttg cccggcacgc gagcgcataa ccactgttct
    70861 gtaacagcat gtttgtcttt ttgcccagca aagctgactt cacgggcatg aattttcagg
    70921 aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc cgtttttgag gattctaacc
    70981 agaatatgct caccttcacc atcaggctca aagcccaaat cttccaccac cacaaagtct
    71041 tccggattgg ctttcagcag cccggtgcct tgcggtttac cgtggaggta agtgagatta
    71101 tcaaactcaa tcattttgtt gccttaatga gtagcgccac cgcttcacag gcaatccctt
    71161 ccccacgtcc ggtaaatcca agtttttccg tagtagtggc tttcacgtta acatcatcca
    71221 tatggcagcc gagatcttcg gcaataaaca cgcgcatttg tggaatgtgc ggcaacatct
    71281 tcggtgcctg agcgatgata gtgacatcga cgttgccaag ggtataaccc ttcgcctgaa
    71341 tacgacgcca ggcttcgcgt agcagctcgc ggctatcggc acctttaaat gccggatcgg
    71401 tatccgggaa cagtttgccg atatccccca gcgccgccgc gccaagcaat gcatcggtca
    71461 acgcatggag cgccacgtcg ccatcagaat gcgccagcaa tcctttttcg taaggaatgc
    71521 gtacgccacc aatgataatt gggccttcac cgccaaaggc gtgtacgtca aaaccgtgtc
    71581 caattcgcat tatgtattct cctgatggat ggttcgggtg aggtaaaact cggccagtgc
    71641 caaatcctcc gggcgcgtga ctttaatgtt atccgcacgg ccttcgacca actgaggatg
    71701 gaatccgcaa tattccagcg ccgaggcttc gtcggtaata gccgcgcctt catttagagc
    71761 gcgcgtcaga cagtcatgta acagctcacg agggaaaaat tgcggcgtca gcgcgtgcca
    71821 taagccgttg cgatcaacgg tatgagcaat ggcatttttg cccggttcgg cacgtttcat
    71881 agtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg ctggtttcgc tcaacgccaa
    71941 caatcgcgcg aggtcatcct gatgcagaca aggacgagcg gcgtcatgca ccaataccca
    72001 ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa tcggcacgct catcaccgcc
    72061 atctacaacg gtgatttgcg gatgattcgc cagaggaagt tgtgcaaaac ggctatcgcc
    72121 aggacttatg gcaatgacga cacgtttcac ccggggatgc gccagcagcg catacaccga
    72181 gtgttcaaga atggtttgat taccgattga gagatattgc ttaggacatt ccgtttgcat
    72241 tcgacggcca aatccggccg ccggaaccac ggcgcaaaca tccaaatgag tggttgccat
    72301 gttaattccc gggctgattt atcgattgtt ttgccccgca gactgtgcgc gcttcgacgc
    72361 gtcaggcacc agacgataaa aagtttcgcc cggcctggtc atgctgagtt cattacgcgc
    72421 acgctcttcg agcgcctcct ggccgccatt gagatcgtca atttcggcaa aaagttgatc
    72481 gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc gccacatcat cattgacgcg
    72541 ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa tactgtagcc agaccagaat
    72601 agccagcaac agcagcgtta gtttacccat cctgccccct gaaaaacggc atcatcatcc
    72661 catgcatccg aagacgactc tacatcctct gttggggata ccgcgacaac gcgggcaaat
    72721 gtaccacatt tgtccattgt tacgtatacc cagggcgtgc agaacataat ctcattatta
    72781 gttacggttt gaattatgaa cagaggagac aagaaagtac aaattagccc agtagccaca
    72841 taaacagtgc gccaaacata atgcctactg tcatcagggt gaaaacaata ctgtagcgta
    72901 gctttccgtc catcaatgaa tgcagcgcaa tccccaccac taccgcaacg ggcatcagcg
    72961 ccagaaagaa aggccaggtg tagataaaga agaacagcgt gttagagcca taaatcaaca
    73021 tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac cacggcacca ggcagtgacc
    73081 atgtggtttc ttcatcctca gtaaggctgt cgttatttgt tagtgtaatg ttatggctat
    73141 tacgcatatt tgatcctgtt actttgacga accgggcatg gaaacccggt ggtgtctcag
    73201 gatctgataa tatcgttctg tctcaacaga tctaataatt gctgtaccaa atttgttact
    73261 aattgttcac cattgagatg aatttctgcc gattcaggcg cttcgtaaac ggaatctatt
    73321 cccgtaaagt tgcgcagttc accggcacgc gctttcttat ataagccttt tggatcgcgg
    73381 gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga taaagcgccc ttctcctacg
    73441 cgttcgcgaa ccatctggcg ttcggcgcgg tgtggcgaga taaatgcggt cagcaccacc
    73501 agtccggctt caaccatcaa attcgccact tcaccgacgc gacggatatt ctctttacga
    73561 tcggcatcgc taaaaccgag atcgctgcat aatccgtggc gaacattgtc gccatccagc
    73621 agatacgtac tgacgccgag tttatgtaac gcctcctcca gcgccccggc gaccgttgat
    73681 ttaccggacc cggagaggcc ggtaaaccac agcactacac cacgatgacc gtggtgtagc
    73741 tcgcgttgtt gcacagtgac cggatggcta tgccagacga cgttttcgtc atgcagcgcc
    73801 attatttctc ccccagcaaa tcgcgcgcgc cccagtgtgg aaagtggcgg cgaaccaggg
    73861 cattcaattc cagttcgaat gcactaaatt cagatggcgc agcagttgcc tggctaactg
    73921 gctcatgcac cataccggca cctacggtca cattgctcag gcgatcgata aaaatcagcc
    73981 caccggtaac cgggttttgc tgataacgat ctaacaccag tggctcgtca aaagtgagat
    74041 ccaccaggcc gatgccgttc agcggcaggt tttcaacttc gcgttgggta aggttattaa
    74101 tatcaacctg ataacgaatg ccatcaacac gagaacgcgt cttcttaccg gcaattttga
    74161 tgtcgtaact ctggcccggg gaaagcggct gttccgccat ccataccaca tccaccgacg
    74221 cgctctgcac agctggtaac gcttcgtctg ccgccagcag cagatcgcca cggctgatgt
    74281 cgatctcatc cgtcagcacc agggtgatag cttctccggc aaaggcttct tcgcgatcac
    74341 catcaaaagt cacgatccgc gcgacgtttg attccacacc agagggcagc acttttacac
    74401 gttgcccgac ttccacgcga ccggatgcca gcgttccggc gtagccacga aaatcgagat
    74461 ttgggcggtt aacgtactgc accgggaagc gcattggctg ggcatccacc actcgctgaa
    74521 tctccacggt ttccagcact tcgagcagtg tcggaccgct gtaccacggc atactttcac
    74581 tttgcgaagc cacgttgtca ccttccagtg cggagagcgg cacaaagcgg atatccagat
    74641 tacccggcag ctgcccggca aaggtcagat aatcttcacg aatacgggtg aacgtctctt
    74701 cactgtaatc caccagatcc attttgttga tcgccacgac cagatgtttg atccccaaca
    74761 gtgtggagat aaaactgtga cgacgggttt gatcgagcac gcctttacgg gcatcgatca
    74821 gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat attgcgggtg tactgctcgt
    74881 gccctggagt gtcggcgata ataaatttac gcttctcggt agagaaatag cggtaggcca
    74941 cgtcaatggt gatgccctgt tcacgctcag cttgcaggcc gtccaccagc agagccagat
    75001 ccagcttttc gccctgggtg ccgtgacgct tactgtcatt atgcagcgat gagagctgat
    75061 cttcatagat ttggcgggta tcgtgcaaca gacgaccaat cagggtactt ttgccgtcat
    75121 cgacgctacc acaggtcaga aaacgcagca ggcttttatg ttgttgcgca atcatccagg
    75181 cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc ggtgttcatc ttaaaaatac
    75241 ccctgacgtt ttttcagctc catagaacca gcttggtcgc ggtcaatcac gcgcccctga
    75301 cgttcactgg tggtggaaac cagcatctct tcgatgatct ccggcagcgt ttgtgcattt
    75361 gactccaccg caccggtcag cggccagcag cccagcgtac ggaaacgcac catccgtttt
    75421 ttaatcactt cgcccggttg caggtcgata cggttgtcat caatcatcat caacataccg
    75481 tcgcgttcca gaaccggacg ttccgcagcg agatacagcg gcacaatgtc gatattttcc
    75541 agccagatgt attgccagat atcctgctcg gtccagttag agagcgggaa aacgcggatg
    75601 ctttcgcctt tgttaatctg cccgttatag ttgtgccaca gctccgggcg ctgatttttc
    75661 gggtcccagc gatggaagcg atcacggaaa gagtagatac gctctttagc acgggatttc
    75721 tcttcgtcgc ggcgcgcacc accgaaggcg gcatcaaaac cgtatttatt cagcgcctgc
    75781 ttcaggcctt cggtcttcat aatatcggta tgtttcgcgc tgccgtgcac gaatggatta
    75841 atccccatcg ccacgccttc cgggttttga tgcaccagca gctcgcagcc gtaggctttc
    75901 gccgtacgat cgcggaactc atacatctca cggaatttcc agccggtatc gacatgcagc
    75961 aacgggaaag gcagcgtacc tggataaaac gccttgcgcg ccagatgcag catgacgctg
    76021 gaatctttac cgatagagta gagcatcacc ggatttgaga attctgccgc cacctcgcga
    76081 ataatgtgga tgctttccgc ctccagttgc cgcaggtgag taagtcgtat ttgatccata
    76141 accgttcctt tgcaataccg ctattttctt gccatcagat gtttcgacta tagggagcgt
    76201 aagagaacga atgaaattac caattagaat gagtagttcc ttaacggaat aacgatttgg
    76261 caaagctaat atcaaaaagt gcttaaggca ccggatttcg ggcgtttagg aagatttgaa
    76321 attgttttag cgcagcggca gtttcatact atggcggtaa aaaaatttgc atggtattta
    76381 aggactcact atgttttccg cattgcgcca ccgtaccgct gccctggcgc tcggcgtatg
    76441 ctttattctc cccgtacacg cctcgtcacc taaacctggc gattttgcca atacacaggc
    76501 gcgacatatt gccactttct ttccgggacg aatgaccgga acacccgcag aaatgttatc
    76561 tgccgattat attcggcaac agtttcagca aatgggttac cgcagtgata ttcgtacgtt
    76621 taatagccga tatatttata ccgcccgcga taaccgcaaa aactggcaca acgtgacggg
    76681 aagtacggtg attgccgctc atgaaggcaa agcgccgcag cagatcatta ttatggcgca
    76741 tctggatacc tatgccccgc agagcgacgc agatgcagat gccaatctcg gcgggctgac
    76801 gttacaagga atggatgata acgccgcagg tttaggtgtc atgctggaac tggcagaacg
    76861 cctgaaaaat acgcctaccg agtatggtat tcgatttgtg gcgaccagtg gagaagagga
    76921 agggaaatta ggcgctgaga atttactcaa gcggatgagt gacaccgaaa agaaaaatac
    76981 gctgctggtg attaatctcg ataacttaat tgttggcgat aaattgtatt tcaacagcgg
    77041 tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac agggcgctgg caattgcgcg
    77101 tagtcatgga attgccgcaa cgaccaatcc gggtttgaat aaaaattatc cgaaaggcac
    77161 tggatgttgt aatgacgcag aaatattcga caaagcgggc attgctgtac tttcggtgga
    77221 agcgacaaac tggaatcttg ggaataaaga tggttatcag caacgcgcaa aaacagccgc
    77281 attccctgcg ggaaatagct ggcatgacgt aagactggat aatcagcaac atattgataa
    77341 agcacttcct ggaagaatag aacgtcgctg ccgtgacgtt atgcggataa tgctaccgct
    77401 ggtgaaggag ttggcgaagg cgtcttgatg ggttggaaaa tgggagctgg gtgttctacc
    77461 gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc cagtgcgccc ggtttatccc
    77521 cgctgatgcg gggaacacag cggcacgctg gattgaacaa atccctgggc cggtttatcc
    77581 ccgctggcgc ggggaacaca aaccgaaaca cacgatcaat ccgaatatga gcggtttatc
    77641 cccgctggcg cggggaacac tttggtgaca gtttttgtca ctgttttggt gacggtttat
    77701 ccccgctggc gcggggaaca cactaagcat acatatctgt ttttaaacaa atttattcca
    77761 catcaacaat ctaccaacta aattcaaaca tttccttatt tttaaagaac acataaccta
    77821 ttgattatca acaggaagaa aagaaaccaa acgtaaccca tccaaatcca ccggaatacg
    77881 tctgttttct ccccaggtct gaaattcaaa acccgactcg gtattggtcg cccaggccat
    77941 caccacattt ccgcaaccag ccagttgggt aatttgctgc cagatcatct cccgaatacg
    78001 ttttgatgta tcaccaacat acacaccggc acgcacttcc agtagccaga ttgcgagccg
    78061 tccacgtaag cgcggaggga cattttctgt aacaaccacg accatgctca tccgccgcgc
    78121 ccccggtgac cactatcacc cagcgtttca ggttcaggga tggcaggcgg taacatatcc
    78181 ggcgcgggtt gtggtggttc aatttcacct gcagcaagga ctttctcaat taacggtatt
    78241 aatttgcccg ttaacttagt gctacggaaa atatcgcgac aggctaatct gacttcttta
    78301 tcaggttctg cgggttgcct cgctgctatt tcaaatgcct ttggcacaac cgaatcaaat
    78361 ttaatgatat cggctatgtc ataaacaaat gaaagcggtt tgccactatg aataaatcca
    78421 atagcgggcg catatcccgc ggctaatact gccgcttcag aaataccgta cagacatgat
    78481 gtggcagcac tgatgcagcg attcacaaca tcgccttttt cccagtcttt aggatcgtat
    78541 ttgcgaccat tccatttcac accatattgt ttcgccagta atgcataggt ctggcgaacg
    78601 cgggatccct caattccccg tagctgatcc actgaacggc gagctggcgg tggctcacga
    78661 aaacgtaatt catacatttt gcgcaccacc ttcaggcgta gatcttccgt taaagccagc
    78721 tttgcctggt agagtaattt atctgcccgc gcccctccgg gttgtccgga agagtaaacg
    78781 cgaacgcccg cttcaccgac ccagaccagc agtgttccca ccgtggcggc cagatgcacc
    78841 gccgcgtggg aaactctcgt tcccggttcg agcataatgc aggcgaccga tcccaccgga
    78901 atgtgcgtgc ggatcccggt tttgtcgatc agcacgaaag cgccgtccag tacgtcgatt
    78961 tgaccgtact ggaggaagat catagaggtg cgatctttta acgggatcgg actcagtggt
    79021 acaaacgtca cacctctgct ccgggtttga tcagcatcag accacagccg aacgcgcggc
    79081 ttttgccata cccctgactg agacgttgca gaaataaccc gggatcggtg accgtaagca
    79141 tccccgtata atccacgcta ctgaactgga tcagttgccg ggagttttcc cgccgcagtt
    79201 gctgttgtcg gtaggcatca accgaggtat ccagcagtgt aaaaccgctt ctctccccct
    79261 gcgctgccag ccagtccagc gccgcctgtt gttgatgcaa ccagacatca cttccttccg
    79321 cctgccccct cacctgccgt ttcgcctcca tcagcagatc gtggcgcttg cccgctttac
    79381 agattgttgg attcgcccgc aggttgaaac aaagttgttg tccggtacgc agctcgggca
    79441 caaatgaccg gcattcgatg gtgaacgttt cactttccgc cgggcgttcc tgcgacagta
    79501 caaaaaagcg aaacgcgccc tggagttctt cccgacgata aagaaattgc ctttctttgc
    79561 cgccagggaa gagatcccac agccactgat gcatcacata ttccccgcga tccaccaaat
    79621 gcagcaactg cgcaggcgaa agctggccgg tatgtaaggt tattcttgag aggtacatgg
    79681 ttcctccttg ctgagccacg gcccctgatt gatggtgcgt tccccaaaca gccactgctg
    79741 acgatttaaa ggaacatccc ggcgacgtaa tattttgctc gcgaccaggc cgtcgtgttc
    79801 cccttcccac cagcattcat cctgaagttt cgggagtgag actttcagtt cgcggaaact
    79861 atcctgatac tgttggtatg cgttacgtaa gacatcagac gcgttgcctt cgagcagtaa
    79921 cggcgcaagt ggtaacgcca gcggatgact ttttcgcccc agataaagcg gaaaaaccgg
    79981 atgacgtaaa ccgtcctgca actgttcaag gctgtaaggc gcatcggggg ttgttgccac
    80041 cgccaccatc caccaggcat cggtgtagta gtcgcgccgg gagataatcg cgctcagaag
    80101 atcaggggcg ctcaactctt cgcgacggct gaaataacgc gctttacgca cctcttttgg
    80161 catctggacc gtgtgataat cccgtgccca gcgcgggtta cggctggcgc aaaccaccag
    80221 tgaatagtgg cggttaaacg cgtttaatcg ttcggtatca tcacgccgaa tccctacccc
    80281 ggcagccagc agccccagca atgctgagcg cgaaggcagt tcatgggtat gacgcacttc
    80341 gccgggggca tcgacgcccc aggatgccat tggcccatga agctgaaaaa tcaaatattg
    80401 gctcattagc cgccccttac gcgcagataa agtccagcac gtccttcatg cttccctgtt
    80461 tgttcatcac gtcaaagctt gcgcattcgg tcttctgttc atagaccgta ttcatatttt
    80521 cgcgaagcgt tgtaatacgc tgcaccgcca catctaactg ccgggtgcca ttaatgggtt
    80581 catagaaagc cgccgccaga gaacgtggtt gttcggtgcc tttttctgcc agcgcccagg
    80641 aggcgtaggc acggctggca aagctgttct gtttgccggt tggggagact ttaagtgcgg
    80701 cttccgtaaa ggcgcgcaag gtctgattag ctaacgcttc gtcaccgccg aggttttcga
    80761 ccagcagatc tttatcgatg cagatatagg tgtagaacag cgcagaaccg aatccggttt
    80821 caccaagatg cccggcaccg gcatcttcag aagcctggcg caaatcatca acggcggtga
    80881 aaaaatcatc ttcgacaatc gtttcactga caccaaatgc atgcgcgacc tggcaggcgg
    80941 cttcaacatt aaattcgggt ttattcgcca gcatacgacc aaacatagcg atatctactg
    81001 ccatgcgatc tttacgtaac aaagcgagat cttcctcttt tggcgcgcgc ttttcttcgg
    81061 ccagttgatg ggccagcgct tttacggcgt caaattctgc cgggctgata tggactaatt
    81121 gttcagtttc ggcgttagtg agcggatctt ttggtttttt gtcgttttta gctttcccaa
    81181 gataatccgc aatttttgcc gcccattcga tggctttttt ctcttcgatg cctttctcaa
    81241 tcaggatagt tgccgcctca cgcgcaatac gcccactgcg aataccaata tggcccgcca
    81301 gtgcctgttc aaaaagtgca gaagtgcgcc acgcacgttt cagactttgc gaggaaacgc
    81361 gcagtcgcgt tgctccaccc aggaccacgg ttttcggcgc tccggtgtca tcacggttaa
    81421 ggttggctgc agggtaagcg gttaacaaat gaagctgaat aaatgtcgtc atgagatagt
    81481 cctttattga ttttgttcgt tatcgacgtc gccagcctgg taatattcca gcgcccagcg
    81541 gatacggata aattcggttg gccgctgatg acggcggtga tgattgagca gatcgtcgct
    81601 ctcctggcac cagcggaaga caccctctgc cagagagtca agattaacgg aaccgtttaa
    81661 taatctgact acgcggcgta actggcgcag taattcatcc ggtgttttta ctgccgacag
    81721 gcgagtaaaa cgcccctttg acattaccgc cgccagctgc gcagcaaaag gcagccgttc
    81781 atcaatggct ttaacattag cgctaagtgc ggctatcagc gccagtgcgg taatacgcca
    81841 ttcaggttca tcctgccact ttatttgtct gttcttcaga aacaggcgaa atccatccgt
    81901 cagacaaaca tcattaaccg tcgtactacg ccgcagactg gcacgttcgc cgcgtttctc
    81961 ctgcaattcc tcatgccatt tgcgcagcgt ggctttgtgc tcctctttta caatactcat
    82021 tcagcagcct cctgcttttt ctccctggcg gctttagcac tttgcttctc cgccgatgtt
    82081 gtaaaatatt tcttgcgcgc ggtcatgacg cgttccaaat caacgggctc ataaggattg
    82141 gtgaatacac gctcgtcaaa atcctgacgt gcgaataacc aaatttcctt ttgccatttg
    82201 ccgagtaatt catccgcatc ctgaccttct tcaatttggc gcactaacct caggaagcga
    82261 tgctgagttt tgttccagaa gtcgatatcc acaaaactga aatcacccct tgcacctttt
    82321 ggatcggaga accatgcttc tttcaatgca ctccgtaaca gactcagaat ccgtgaagcc
    82381 gtttgcgcag ccagccgcag cttcggtatc tggccttctt ttttattgag cagcagcggg
    82441 aaatggtgtt cgtaccaaca gcgcgctttc atgttgtcga aatcataacc aaatccccac
    82501 aggcccactt ttgcctgttt cagactgctg gcattaaaga gtttcaccac cagcgcggga
    82561 agttccgtat tgttttctga cttacccgtt tcgataaggc ctaaccagtc gcgccagatt
    82621 aaaccgcccg gttgtggttt aacggagtaa aactcaccgc cctctttaag tggtacacgg
    82681 taaggcgtta agggatgctg ccacatggca taattcgcac cgtaattttt ggtagtcatc
    82741 aaactcagaa gcgcgtcact ctgctcaccg caaatatcgc agttgccgac tgtcgtggta
    82801 ttaaaatcaa tacgaatacg ccgcggcatt ccccagtacg cctggagttt attgacctga
    82861 tcatcggtta ccaccgcacc ggccagttcg ctggtacgcg tcgggccaag ccaggggaaa
    82921 accagatcgt caaatttttt gggtagcggt aagtcggctt catcctgcgg catcacgttg
    82981 agccacagtt tgcgccacaa gggggcttgt tgattgccct gatactcctg caattcaatc
    83041 agagtcgtca tcggcccacc gccgcgtaaa ccggtgcgat agcctttgcc acctgacggc
    83101 gcatttaact gtagggagaa cagagctaac gcagaacaat gagagcatac gtgttcagtc
    83161 acgccacgct taataaagtg gtctttatta aacttcgttg tttgagcgcc gggaatctca
    83221 ggcagtagcg aagcgacctg aactttatcg cccatgagca cctcgaaatc ctgcataaat
    83281 gaaggtgaat ctgggccaaa ctggaaaggg tgttctaatg acagcaatgc ttcccgtagc
    83341 ttttcagctt ccagcccgtc ttcccagata tcatcccaac gacgataatc ttttggcgcg
    83401 aaactgcttt gtagtaaccc cagcaaaaac tgccatgccg ccccctggag atctgcccgc
    83461 ggcgcagcga tatcgacaac attttcatcc gccagatcga ctggcgccag cttgcctgtt
    83521 gttccgtctt taaaacgaac gggcaaccac ggggttgtca gaagtgaaaa cgagttcata
    83581 tgtatcctct cgtaaaaaag accatccctg gtcggttgtc catcattcca tccttttcgg
    83641 tgtatcaact attcagagag tcctgtcgca ggtatctcaa tcaaccttgc caatcaatcc
    83701 ctccctggcc gaatacccgc aactttcgtc atcagtgact aaaatcacgt ttgccatttc
    83761 cggatcttgc cgctgttcaa tgcaccactg cctgaacgct tccccttcca gtaaagaaaa
    83821 ctcatcccga tgttttttcc accagcttcg acgcactctg acaacgctca tttcccatgc
    83881 gtgagcaccg gtggcataag gcttcaccac accggcaata caggtagcca gccacaggga
    83941 aacagattcc tcagccagac gtgtcgacag cttttccgga aggtaatcgt tgatattggc
    84001 ggcatagcca ggcttgaagt tcaggacaaa ctttttagcc attgcgcgat cgcagtaata
    84061 tttgcccact tgctcctgct cgctgcgggc aaatccttcc ggcattacca cgtcctcacc
    84121 gtagactgat tcaataagaa ggcgggctgc gtgtggcatt tgaatagcgc cttgctcacg
    84181 cagtacacgc tgcgtcagcc agattcgtcc atgatcggga tagacatatg cactgttacg
    84241 catggcactg ccgaaccatt cgtcaccagg agcgtcgtcc cagacggggg ccagaatcag
    84301 caattcagga ggggaacgct cgtcttttcc gtcacgcttt aactgaccat taatatcgcg
    84361 gatatgccgc tgtaatcgcc ccgctcgctg aatcagcaaa tcaacagggg ccaggtcgga
    84421 gatcatttcg tccaggtcac aatcaacgct ctgctctaag acctgagtac aaatgaggac
    84481 ttttccggca cgctgtgaac cgtcttcttt accaaagcgt gccagcgtct ccatttcaat
    84541 tcgctggcga tcgctaaaag caaagcggct atgaaagagt gaaaggctgg aagcgggaat
    84601 gacgccgcgg gcaagcagct gacgatgaac cttaatagcg tcatcgacag aattccggat
    84661 ccaggcgatg cattttccct gacttaccgc cgattcgata cgcgcaatac tctcttgttc
    84721 actatgaagc caacccacgc tgacgctacg ctcaacgtct ttgcgcgtcg ctacccggtg
    84781 tgagttcaca tcggatttcg tgacatgcgt cagccagggg taatcatcct tttcaaggaa
    84841 cggagcttct tgctggccct ctgtgccacg cgcaaaggcg gcgacgagtt tgtcgcgctg
    84901 ctgttgggat aacgtagcag aaagcaaaat gacgctgttt ccgccacgcg cctgccgctc
    84961 gatcagccct tcaagaatgc acgacatgta agcatcacag gcatggatct catcagccag
    85021 caggattttg ttactcaacc ccagaagccg cagattatta tgtttaaacg gcatcactgc
    85081 catcatcgcc tgatccagcg tgccgacgcc aatttcagcc agtagcgcct tcttgttact
    85141 gttggcaaac caggccgcac atccctgact gaatgtttgt tcatccggtt cttctgaccc
    85201 gactaaatca ccggaccaga gtgattcatt gaagcggtcc attaatgtgc gggcactgtg
    85261 tgccagcacc aagctggggc gggactctgg cgaatagaaa gcaagccagg ttttgaccag
    85321 ccgatcgtac atggcattgg ccgttgccat tgttggcagg ccaaaaaaca aaccctgtgc
    85381 tttcctcgca gccatcaacc tgtgcgccag gataagcgcc gcttctgttt tacctgcgcc
    85441 agtcacgtct tccagaataa ataactgtgg ccctggctgg ctgatatcca gatccagtac
    85501 cttttgctgt aatggtgtcg ggtgctcaat aaaaggaaac agcgtattaa ttccggtgaa
    85561 aggtgcggtt tctgcttttg gaggaaagac ggttaaggcg ttttgagcct gaactaaagt
    85621 tttctgccag taatctttaa tatccattgg gtgtgcgacg cgtggaaaaa atcgcgttga
    85681 cgaacccgtc cagtctgcga gtacgactgt tgcagagata taccaggaaa gttgttttaa
    85741 aagttcaacg ccctcgtcat catcccagaa tgtgggaatc tctatgagcg gaaacagtgc
    85801 cttgatttca aggagaaaat ctcgcgcggc agctttgtct tcaggcagaa aattatccag
    85861 ctcatcaata cggtcaggtg gtcgaccatg atgcccggta gttatggaca tccacatctc
    85921 tattacacgt gtaagtttac gagaagagag tgaagatgaa ggaagcaact cctcacattc
    85981 acttaaataa taattccaca gccagtaacc cagcgttgaa tgagagatct tttcgtaatt
    86041 ctttctggaa ccttccggaa tcttgagttc aggggccagg taaagttgct gaaaagagcg
    86101 ggcaaatttt ccaatatcgt gccagcacag caaccaagcg aaaaattgag ccgcctgttc
    86161 cttgtcagaa atccctaatt gacgaaagta atcagccagc ccgaagcaat ttcttttaac
    86221 cattaaatag cccattgcgg ccacatccag cgaatgccag caaagaaggt gatagccgtc
    86281 gccaccctct ttctcgccac gtcgggtttt tccccagaaa tcaaagaaag tcacaatatt
    86341 tttatccttc agtaaactta aaggatattt ac
//
LOCUS       DAZFDC010000006        81540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFDC010000006 DAZFDC010000000
VERSION     DAZFDC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.006"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(102..1052)
                     /gene="dusC"
                     /locus_tag="ACHJ48_000477"
     CDS             complement(102..1052)
                     /gene="dusC"
                     /locus_tag="ACHJ48_000477"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO4706104.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGC
                     DSVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR"
     gene            complement(1212..2405)
                     /locus_tag="ACHJ48_000478"
     CDS             complement(1212..2405)
                     /locus_tag="ACHJ48_000478"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO4706105.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(2420..3064)
                     /gene="maiA"
                     /locus_tag="ACHJ48_000479"
     CDS             complement(2420..3064)
                     /gene="maiA"
                     /locus_tag="ACHJ48_000479"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO4706106.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(3073..3774)
                     /locus_tag="ACHJ48_000480"
     CDS             complement(3073..3774)
                     /locus_tag="ACHJ48_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4706107.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            complement(3789..4817)
                     /gene="gtdA"
                     /locus_tag="ACHJ48_000481"
     CDS             complement(3789..4817)
                     /gene="gtdA"
                     /locus_tag="ACHJ48_000481"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO4706108.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(4829..6187)
                     /locus_tag="ACHJ48_000482"
     CDS             complement(4829..6187)
                     /locus_tag="ACHJ48_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4706109.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLTLSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            6314..7222
                     /locus_tag="ACHJ48_000483"
     CDS             6314..7222
                     /locus_tag="ACHJ48_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4706110.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            7353..7751
                     /gene="yohJ"
                     /locus_tag="ACHJ48_000484"
     CDS             7353..7751
                     /gene="yohJ"
                     /locus_tag="ACHJ48_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO4706111.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            7748..8443
                     /gene="yohK"
                     /locus_tag="ACHJ48_000485"
     CDS             7748..8443
                     /gene="yohK"
                     /locus_tag="ACHJ48_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO4706112.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APILFPIILAVMG"
     gene            8573..9457
                     /gene="cdd"
                     /locus_tag="ACHJ48_000486"
     CDS             8573..9457
                     /gene="cdd"
                     /locus_tag="ACHJ48_000486"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO4706113.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LADAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            9607..10326
                     /gene="sanA"
                     /locus_tag="ACHJ48_000487"
     CDS             9607..10326
                     /gene="sanA"
                     /locus_tag="ACHJ48_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO4706114.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            10329..10568
                     /gene="yeiS"
                     /locus_tag="ACHJ48_000488"
     CDS             10329..10568
                     /gene="yeiS"
                     /locus_tag="ACHJ48_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO4706115.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            10762..12000
                     /gene="preT"
                     /locus_tag="ACHJ48_000489"
     CDS             10762..12000
                     /gene="preT"
                     /locus_tag="ACHJ48_000489"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO4706116.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDDFAKLEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            11994..13229
                     /gene="preA"
                     /locus_tag="ACHJ48_000490"
     CDS             11994..13229
                     /gene="preA"
                     /locus_tag="ACHJ48_000490"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO4706117.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(13472..14482)
                     /gene="mglC"
                     /locus_tag="ACHJ48_000491"
     CDS             complement(13472..14482)
                     /gene="mglC"
                     /locus_tag="ACHJ48_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO4706118.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQAPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(14498..16018)
                     /gene="mglA"
                     /locus_tag="ACHJ48_000492"
     CDS             complement(14498..16018)
                     /gene="mglA"
                     /locus_tag="ACHJ48_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO4706119.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYKKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(16079..17077)
                     /gene="mglB"
                     /locus_tag="ACHJ48_000493"
     CDS             complement(16079..17077)
                     /gene="mglB"
                     /locus_tag="ACHJ48_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO4706120.1"
                     /translation="MNKKVLTLSAVMASMLFGATAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(17356..18396)
                     /gene="galS"
                     /locus_tag="ACHJ48_000494"
     CDS             complement(17356..18396)
                     /gene="galS"
                     /locus_tag="ACHJ48_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO4706121.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(18538..19695)
                     /gene="yeiB"
                     /locus_tag="ACHJ48_000495"
     CDS             complement(18538..19695)
                     /gene="yeiB"
                     /locus_tag="ACHJ48_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO4706122.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(19712..20380)
                     /gene="folE"
                     /locus_tag="ACHJ48_000496"
     CDS             complement(19712..20380)
                     /gene="folE"
                     /locus_tag="ACHJ48_000496"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO4706123.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            20638..21474
                     /gene="yeiG"
                     /locus_tag="ACHJ48_000497"
     CDS             20638..21474
                     /gene="yeiG"
                     /locus_tag="ACHJ48_000497"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4706124.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(21506..23485)
                     /gene="cirA"
                     /locus_tag="ACHJ48_000498"
     CDS             complement(21506..23485)
                     /gene="cirA"
                     /locus_tag="ACHJ48_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO4706125.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(23777..25246)
                     /gene="lysP"
                     /locus_tag="ACHJ48_000499"
     CDS             complement(23777..25246)
                     /gene="lysP"
                     /locus_tag="ACHJ48_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO4706126.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(25451..26332)
                     /gene="yieE"
                     /locus_tag="ACHJ48_000500"
     CDS             complement(25451..26332)
                     /gene="yieE"
                     /locus_tag="ACHJ48_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO4706127.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            26431..27480
                     /gene="yeiH"
                     /locus_tag="ACHJ48_000501"
     CDS             26431..27480
                     /gene="yeiH"
                     /locus_tag="ACHJ48_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO4706128.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            27554..28411
                     /gene="nfo"
                     /locus_tag="ACHJ48_000502"
     CDS             27554..28411
                     /gene="nfo"
                     /locus_tag="ACHJ48_000502"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO4706129.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            28414..29502
                     /gene="yeiI"
                     /locus_tag="ACHJ48_000503"
     CDS             28414..29502
                     /gene="yeiI"
                     /locus_tag="ACHJ48_000503"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4706130.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(29558..30808)
                     /gene="nupX"
                     /locus_tag="ACHJ48_000504"
     CDS             complement(29558..30808)
                     /gene="nupX"
                     /locus_tag="ACHJ48_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4706131.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESIVGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(30908..31849)
                     /gene="rihB"
                     /locus_tag="ACHJ48_000505"
     CDS             complement(30908..31849)
                     /gene="rihB"
                     /locus_tag="ACHJ48_000505"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO4706132.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGDTGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDSDWFWGLVEECVRGYIKTH"
     gene            31979..32677
                     /gene="yeiL"
                     /locus_tag="ACHJ48_000506"
     CDS             31979..32677
                     /gene="yeiL"
                     /locus_tag="ACHJ48_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO4706133.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(32748..33998)
                     /locus_tag="ACHJ48_000507"
     CDS             complement(32748..33998)
                     /locus_tag="ACHJ48_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO4706134.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVAAALLLQIALGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATELSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(34092..35030)
                     /gene="psuG"
                     /locus_tag="ACHJ48_000508"
     CDS             complement(34092..35030)
                     /gene="psuG"
                     /locus_tag="ACHJ48_000508"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO4706135.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAVIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(35018..35959)
                     /gene="psuK"
                     /locus_tag="ACHJ48_000509"
     CDS             complement(35018..35959)
                     /gene="psuK"
                     /locus_tag="ACHJ48_000509"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO4706136.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRERLSQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(36383..38074)
                     /gene="fruA"
                     /locus_tag="ACHJ48_000510"
     CDS             complement(36383..38074)
                     /gene="fruA"
                     /locus_tag="ACHJ48_000510"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO4706137.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKNESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(38091..39029)
                     /gene="fruK"
                     /locus_tag="ACHJ48_000511"
     CDS             complement(38091..39029)
                     /gene="fruK"
                     /locus_tag="ACHJ48_000511"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO4706138.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(39029..40159)
                     /gene="fruB"
                     /locus_tag="ACHJ48_000512"
     CDS             complement(39029..40159)
                     /gene="fruB"
                     /locus_tag="ACHJ48_000512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO4706139.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            40527..41708
                     /gene="setB"
                     /locus_tag="ACHJ48_000513"
     CDS             40527..41708
                     /gene="setB"
                     /locus_tag="ACHJ48_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO4706140.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGVLVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGTGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(41705..41959)
                     /gene="yeiW"
                     /locus_tag="ACHJ48_000514"
     CDS             complement(41705..41959)
                     /gene="yeiW"
                     /locus_tag="ACHJ48_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4706141.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            42114..42686
                     /gene="yeiP"
                     /locus_tag="ACHJ48_000515"
     CDS             42114..42686
                     /gene="yeiP"
                     /locus_tag="ACHJ48_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO4706142.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            42909..44375
                     /gene="yeiQ"
                     /locus_tag="ACHJ48_000516"
     CDS             42909..44375
                     /gene="yeiQ"
                     /locus_tag="ACHJ48_000516"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4706143.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRDLVAGSISEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQARQQIAQFGAHQALLNTLKI"
     gene            44493..45479
                     /gene="yeiR"
                     /locus_tag="ACHJ48_000517"
     CDS             44493..45479
                     /gene="yeiR"
                     /locus_tag="ACHJ48_000517"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO4706144.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVAGHLLDLPRHNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            45518..46231
                     /gene="lpxT"
                     /locus_tag="ACHJ48_000518"
     CDS             45518..46231
                     /gene="lpxT"
                     /locus_tag="ACHJ48_000518"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO4706145.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            46644..47210
                     /gene="mepS"
                     /locus_tag="ACHJ48_000519"
     CDS             46644..47210
                     /gene="mepS"
                     /locus_tag="ACHJ48_000519"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO4706146.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            47391..48947
                     /gene="pdeN"
                     /locus_tag="ACHJ48_000520"
     CDS             47391..48947
                     /gene="pdeN"
                     /locus_tag="ACHJ48_000520"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4706147.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCKQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKANIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            49029..50843
                     /gene="yejA"
                     /locus_tag="ACHJ48_000521"
     CDS             49029..50843
                     /gene="yejA"
                     /locus_tag="ACHJ48_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO4706148.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYSGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDHSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            50844..51938
                     /gene="yejB"
                     /locus_tag="ACHJ48_000522"
     CDS             50844..51938
                     /gene="yejB"
                     /locus_tag="ACHJ48_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO4706149.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            51938..52962
                     /gene="yejE"
                     /locus_tag="ACHJ48_000523"
                     /pseudo
     CDS             51938..52962
                     /gene="yejE"
                     /locus_tag="ACHJ48_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            52964..54553
                     /gene="yejF"
                     /locus_tag="ACHJ48_000524"
     CDS             52964..54553
                     /gene="yejF"
                     /locus_tag="ACHJ48_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO4706150.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIDVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(54557..54901)
                     /gene="yejG"
                     /locus_tag="ACHJ48_000525"
     CDS             complement(54557..54901)
                     /gene="yejG"
                     /locus_tag="ACHJ48_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO4706151.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALGDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(55234..56424)
                     /gene="bcr"
                     /locus_tag="ACHJ48_000526"
     CDS             complement(55234..56424)
                     /gene="bcr"
                     /locus_tag="ACHJ48_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO4706152.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(56452..57147)
                     /gene="rsuA"
                     /locus_tag="ACHJ48_000527"
     CDS             complement(56452..57147)
                     /gene="rsuA"
                     /locus_tag="ACHJ48_000527"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO4706153.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            57296..59056
                     /gene="radD"
                     /locus_tag="ACHJ48_000528"
     CDS             57296..59056
                     /gene="radD"
                     /locus_tag="ACHJ48_000528"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4706154.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            59181..59465
                     /gene="rplY"
                     /locus_tag="ACHJ48_000529"
     CDS             59181..59465
                     /gene="rplY"
                     /locus_tag="ACHJ48_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO4706155.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNIQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(59604..60611)
                     /gene="yejK"
                     /locus_tag="ACHJ48_000530"
     CDS             complement(59604..60611)
                     /gene="yejK"
                     /locus_tag="ACHJ48_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO4706156.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            60793..61020
                     /gene="yejL"
                     /locus_tag="ACHJ48_000531"
     CDS             60793..61020
                     /gene="yejL"
                     /locus_tag="ACHJ48_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO4706157.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            61040..62800
                     /gene="yejM"
                     /locus_tag="ACHJ48_000532"
     CDS             61040..62800
                     /gene="yejM"
                     /locus_tag="ACHJ48_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO4706158.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            62875..62951
                     /locus_tag="ACHJ48_000533"
     tRNA            62875..62951
                     /locus_tag="ACHJ48_000533"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62909..62911,aa:Pro,seq:ggg)
     gene            complement(63052..65643)
                     /gene="yejO"
                     /locus_tag="ACHJ48_000534"
     CDS             complement(63052..65643)
                     /gene="yejO"
                     /locus_tag="ACHJ48_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFO4706159.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     LRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMDESVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            65963..66610
                     /gene="narP"
                     /locus_tag="ACHJ48_000535"
     CDS             65963..66610
                     /gene="narP"
                     /locus_tag="ACHJ48_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO4706160.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(66645..67697)
                     /gene="ccmH"
                     /locus_tag="ACHJ48_000536"
     CDS             complement(66645..67697)
                     /gene="ccmH"
                     /locus_tag="ACHJ48_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO4706161.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDATLGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(67694..68251)
                     /gene="dsbE"
                     /locus_tag="ACHJ48_000537"
     CDS             complement(67694..68251)
                     /gene="dsbE"
                     /locus_tag="ACHJ48_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO4706162.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(68248..70191)
                     /gene="ccmF"
                     /locus_tag="ACHJ48_000538"
     CDS             complement(68248..70191)
                     /gene="ccmF"
                     /locus_tag="ACHJ48_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO4706163.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(70188..70667)
                     /gene="ccmE"
                     /locus_tag="ACHJ48_000539"
     CDS             complement(70188..70667)
                     /gene="ccmE"
                     /locus_tag="ACHJ48_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO4706164.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(70664..70873)
                     /gene="ccmD"
                     /locus_tag="ACHJ48_000540"
     CDS             complement(70664..70873)
                     /gene="ccmD"
                     /locus_tag="ACHJ48_000540"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO4706165.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(70870..71607)
                     /gene="ccmC"
                     /locus_tag="ACHJ48_000541"
     CDS             complement(70870..71607)
                     /gene="ccmC"
                     /locus_tag="ACHJ48_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO4706166.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(71649..72311)
                     /gene="ccmB"
                     /locus_tag="ACHJ48_000542"
     CDS             complement(71649..72311)
                     /gene="ccmB"
                     /locus_tag="ACHJ48_000542"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO4706167.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(72308..72931)
                     /gene="ccmA"
                     /locus_tag="ACHJ48_000543"
     CDS             complement(72308..72931)
                     /gene="ccmA"
                     /locus_tag="ACHJ48_000543"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO4706168.1"
                     /translation="MGMLEVRELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAV"
     gene            complement(72944..73546)
                     /gene="napC"
                     /locus_tag="ACHJ48_000544"
     CDS             complement(72944..73546)
                     /gene="napC"
                     /locus_tag="ACHJ48_000544"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO4706169.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(73556..74005)
                     /gene="napB"
                     /locus_tag="ACHJ48_000545"
     CDS             complement(73556..74005)
                     /gene="napB"
                     /locus_tag="ACHJ48_000545"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO4706170.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(74002..74865)
                     /gene="napH"
                     /locus_tag="ACHJ48_000546"
     CDS             complement(74002..74865)
                     /gene="napH"
                     /locus_tag="ACHJ48_000546"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO4706171.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(74852..75547)
                     /gene="napG"
                     /locus_tag="ACHJ48_000547"
     CDS             complement(74852..75547)
                     /gene="napG"
                     /locus_tag="ACHJ48_000547"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO4706172.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLVVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(75554..78040)
                     /gene="napA"
                     /locus_tag="ACHJ48_000548"
     CDS             complement(75554..78040)
                     /gene="napA"
                     /locus_tag="ACHJ48_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO4706173.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(78037..78300)
                     /gene="napD"
                     /locus_tag="ACHJ48_000549"
     CDS             complement(78037..78300)
                     /gene="napD"
                     /locus_tag="ACHJ48_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO4706174.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(78290..78784)
                     /gene="napF"
                     /locus_tag="ACHJ48_000550"
     CDS             complement(78290..78784)
                     /gene="napF"
                     /locus_tag="ACHJ48_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO4706175.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            78893..79057
                     /gene="yojO"
                     /locus_tag="ACHJ48_000551"
     CDS             78893..79057
                     /gene="yojO"
                     /locus_tag="ACHJ48_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO4706176.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     FLLVCSLIRI"
     gene            79191..79679
                     /gene="eco"
                     /locus_tag="ACHJ48_000552"
     CDS             79191..79679
                     /gene="eco"
                     /locus_tag="ACHJ48_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO4706177.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(79829..81475)
                     /gene="mqo"
                     /locus_tag="ACHJ48_000553"
     CDS             complement(79829..81475)
                     /gene="mqo"
                     /locus_tag="ACHJ48_000553"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO4706178.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT        19872 a        22011 c        20143 g        19514 t
ORIGIN      
        1 tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc gcaacaagat gatgaagctg
       61 aataagcgat tcattgtttc tgataaactg cgattccact attatcgcag cttaccgata
      121 tcaattgcct gaatagccct tgcaatatca ggggaattat tcaacacacg aacatgctga
      181 aataattctg ttgcttcatc gtattcttta cgcaaataac tcaaccactg tttaatccgc
      241 gctacgtgat ataacccggt atcgccctgc ttttccagac gggtatattt ttgcagcaaa
      301 gcaaccacct ccggccacgg cattcgcggt tcgttatatt ttactacccg gctcaggttg
      361 ggaatattga gcgccccgcg accaatcatc actgagtcgc agccgctgat cgccatgcat
      421 tcttgcgcgc tctgccagtc ccagatttcg ccgttggcaa tcaccggaat attcagccgc
      481 tggcgaatct cgccaatcgc ctgccagtca atatgctccg cgcggtaacc ctgctctttt
      541 gtccgtccat gcaccaccag ctccgtagca ccagcctgtt gaaccgcatc ggcgatttca
      601 aatttcttct caccgctgtc ccagcccaga cgcactttca cgctgacggg caaatgcgcc
      661 ggtacagctt cacgcatcgc ttttgcaccc tggtagatga gttcaggatc tttgagtaac
      721 gtcgccccac caccgctacc gttaaccgtt ttcgacgggc agccgcaatt gagatccacg
      781 ccccaggaac ctaattccac cgcacgggcg gcgttctctg ccagccattg tgggaactga
      841 cccaacaact gcacgcgcac cagcgtaccg gatggtgtcc ggctggcgtt ttgtagctca
      901 gggcaaatgc gatgaaagac tttcaccggc agcagttgat ccaccacgcg gacgaactcg
      961 gtgatgcaca gatcgtagtc gttaacttcg gtcagcaatt cacgcaccag agagtcaagc
     1021 actccctcca tcggagccag taacacacgc atatcatcac ccgcaaaaaa atgaggcgct
     1081 atgttagcgc ctcctgtgtt tgcagacaaa ctgacttact gattaattcg ggcaagattt
     1141 ttaccctcac cctaaccctc tcccgaaggg gcgaggggac cgcccgtgcg cctgattggg
     1201 tgaagaagtg cttagcgtgg ttgcaaacag ttatcttctt tccagccgta caaccactcc
     1261 atgccgcgat aaaattctgc ctgcgatttc cctttccaca gcagattacg tacctggcgt
     1321 tctacccctg cggcgtggta aacccgcccc atttcacggg tcgaccagac gatgcgcgcg
     1381 gtgcgcggaa ttcgtaccga ctcatacagc gcaaaggctt gcgccgcatc accctcacac
     1441 tgcgccagcg ccttacccag cgttactgca tcttccagcg ccatacaggc tccctgcgcc
     1501 atatattgcg ccaccggatg ggccgaatcg cccacaagcg ttatgcgctt cgttccccac
     1561 ttcgccaccg gttcacggtc agcggttgac cagcgtcgcc atgaagtcgg tttatccaac
     1621 atctggcgcg gacgggggtg aatcccttca aagtaagaga gcacttcctc tttactacca
     1681 tccttcacgc cccactcttc ctgctggcgg ctgtggaatg ttaccaccag gttatactgc
     1741 ttgccgccgc gcaaagggta atgcaccaag tgacaatgcg gccccgccca cagtaccggt
     1801 gcgttaatgc gcaaatcttc cggcatatcg gcacgatcga taaccgcacg gtagacaaca
     1861 tgtccggtga cacgcggcgc atcgcccaat aagctctggc gcaccactga cttcacgccg
     1921 tcacagccaa tcagaatatc gccggtccag ctattgccgc gatcgtcaaa cacggtgaca
     1981 tcgtcttccg tctcgcgaat atccacgacg ttggtggagg tgtgatattc cacgtccgga
     2041 tgcgtcagta ccgcttccca caccgaggcg tgaatatcaa cccggtggat caccgcgtaa
     2101 gggccgccga aatggtcacg aaaggcctgc ccggtttcaa tgcacaccac ttcttcggcg
     2161 ttcaccgcat ccatcatggt aatgtgatcg gtaaacaccg cgcgctggcg ggcaacttcg
     2221 ccgacgccga ggctgtccag cgccgaaaac gcatttggcc ccaactggat gcccgcgcca
     2281 atctcaccga tctcatgcgc cttttccagc agcaccactt tgatccccag gcgggccagt
     2341 gacagcgcag ttgccgcacc gccgatcccg cctcccacaa ttactgcgcg tgtcactttc
     2401 gtcattatag ttctcctggt caggctgaaa ttttatcttg ttgattttct ggcgctgcgg
     2461 caatgaacgc tggcagctgt gtgcaggcgt cgtacaccgc tttgcagcgt ggatacccgc
     2521 tcaaatcgca gttcatgcgt agcgcgttcg cccactgcgg gacaaggcag caatctgcca
     2581 gcgtcggcgt ctcgcccaca caaaattgcc ccgactggtt ctggcgcaac agttgttcca
     2641 ccgcgcttaa cccttgctgg atccagtgcg cataccaacg ttttttttct tcttcactga
     2701 cgttcagttc ttcggtcagg tagcgcagca cgcgcaagtt attcaatgga tgaatatcac
     2761 aagcgatggc atagacaatt tccagcacct tgttgcgggc cggctcttcc tgcggcacca
     2821 gcggaacttg cgggtagtgc ctctccagcc agtcaataat cgccagcgat tgtccgagtg
     2881 attcgccctc gtcagtcagc agcgtcggca ccagcccgac cggattcatc cgccgatagg
     2941 ccagttcatt ttgctgacca atacggatat tcaccccttc ggtctggtaa ttaatgcctt
     3001 tcagcgccag cgcaatgcgt acccggtacg acgctgaact attaaaaaaa ctgtacagct
     3061 tcatcatgca cctcagacaa tcttcaccgc gataggcgtt aagccctcga cgttgcctgt
     3121 gatcacatca cctttcacta ccgggccaac cccttccggc gtaccggtga agatcagatc
     3181 gcccggttgc agctcaaaga agcctgacag atagcagatg gtttcattca ccgaccagat
     3241 gagatggcga atatcgctgc gctgatgatc ttcgccgtta acctgtaacc agattggcgc
     3301 attatcaata ccgccgatat cgcgggcttt gtgcagcggc gcgatgggcg ctgagagatc
     3361 aaacgcttta ccgatttccc acgggcggcc catctggcgc atttccattt gacggtcacg
     3421 ccgagtcata tcaagacccg tggcataacc ccagacatat tcatgggcgt gttccagcgg
     3481 gatatcgctg ccttttttac caatcgccac caccagttca atttcgtaat ggtagttatc
     3541 ggtctgtgcc ggatacggca gcgccagcgt ttcaccagcg gcaaccggaa caacggcatc
     3601 cgccggtttg cagaagaaga atggcggttc acgatccgga tcaaagccca tttcgcgggc
     3661 gtgagcggca tagttacgtc cgacgcagta aacgcggcgc acggggaatt gttcatcact
     3721 gccaacaacc ggtacggtaa cgggcgcttg cggttcgaat acgtattgag tcatttttct
     3781 ctcctaaatt aataacgcgc ttcgcggaac agccccaggg cttcttgtac cggtctgtcc
     3841 gaaaaactaa ataaaacgga atcttgcgtg gtctggaacg acacgccatg ccaggtcggc
     3901 accacgaaga tatcttttgc actaaagctg aaggtttcat tgccgatgat gacctgcccg
     3961 ctaccttcca ccacatggta gatggtgctg tcagtggtac gcgcaacgcg tgaggcaaac
     4021 cctttcggca gcagttgcag gaaagcgccc atcgacggca tcggatagcc gccggtgacc
     4081 gggttgacgt agcgcatttt gtagccatcc cactcgtcgg catcgcccag tcgggttaaa
     4141 tcgtgcagca cttcgcggct gcggtcataa cgatagttaa agatcggcga ggagttcccc
     4201 gtctgatgcc gcagcggcaa catattggca gcgtaacgcg gcagataatc tccctcttta
     4261 cgcgttaccg gttgttgctc ttccggataa tcttcggcaa aaccgcagcc cagaatattc
     4321 actaatggca gatccagccc atcgagccag ataaccggtt cgtcgccagg gttaccgtga
     4381 tcgtgccagc gccactgcgg ggtcaggata aaatcgcctt cattcattgg cgtgcgttcg
     4441 ccatcaacgg cggtaaatgc ccctttgcct tcaacaataa aacgcagcgc cgactgatta
     4501 tgacgatgac tcggtgccac ttcgcccggc atgatcagtt gcaaaccggc atataaagtc
     4561 gcggtaatgg aagactgccc gcgcagcgcc gggttttcca gcaccagcac gcggcgcacg
     4621 gcctctttcg caccaatcag gccgccgctc tccagtagca gcgggcggat ctcctgataa
     4681 ttccagtacg ctggcgcaca gttagcgttg ggcgttttcg gcaccagatg gtgcagtgac
     4741 tcccacagcg gggtcaggtt ttgcccggaa atatgctgat aaaattgctc gcggctattt
     4801 tgattgttat cagtcattga gatgctcctt attcgttcac aacgcctgcg cgaggcagtg
     4861 agtcagggac agaggtcgac tggcgaacca cggtaatcag cagggttaac atcaccgcac
     4921 tgatcgccgc aggcacagcg ataatgaaaa acagggtgtc gaaagagaaa ttcatcgcca
     4981 tcatcacgcc gccagacagc gaaccaacaa tcgcgccgca gcggccgata gcatttgacc
     5041 agctcacgcc cgtggctcga ctttgtgttg gataaagtgt tgcggtcagc gcattcagcc
     5101 ccacctgcga tccgctaatg ccaatgccag tgccgaaaat cgccagcgcc atcaaccata
     5161 atccatcctg gcttaagcca atcatcacga tgcatatagc gcctatggca tagctcagcg
     5221 tcagcacgcg gaaaggattg aacttatcca tcagcacacc cagcgccagc gcgcctaacg
     5281 taccaccaat ctggaacgcg gcggtgaccc aggacgcgtg ttgcaggtcg atgccgcgat
     5341 ggttcagtag cgtcggcatc cagcttgaga gcaggtaaat aatcagcagg ctcataaaga
     5401 acactaccca cagcatcaac gtgatgggta actgacgtcc cataaacagt tgacgaatgc
     5461 tgccttttgt aacggaggct gattcaatga gataaaagtg ggtatcgaca taacgctcgc
     5521 gggtgatggc gctgacggtt ttcgcaatca ccgcctgcgg tagctggcgg cgcacctgcc
     5581 agcgtggcga ttccggcaga actaccagca gtgcgacaaa cagcatcagc ggtaacacgc
     5641 cccccaacac cagaatgccg tgccagccaa tcaccggcac cagctgcgca ctgacaatcc
     5701 cgccaaacgc cgaacccagc gtaaagccgc aaaacatcag cgtcaccagc gcgccgcggc
     5761 ggcgggcagg cagatactcc gaggtcatgg tgatggtgtt cggcatcgcg ccgcccagac
     5821 ccaggccggt gagaaagcgc aggaagacca gcgtttgcag atccggtgag aaagccgagg
     5881 cgaggcttaa cgcgccaaac aaaaagacgc acagctcaat cacccgcttg cgaccaaagc
     5941 gatcggacaa cgggccacaa agcagtgcgc ctgcggttaa tcctagcaga cccgcgccaa
     6001 aaagcggggc gagatcgcct gcggtgagtt gccagtgagt gcgaatatca ggggcgataa
     6061 atccgatagc cgcggtgtca aaaccatcga gcattaccac cagaaaacag cagatgataa
     6121 cgcgccactg catcttgccg accggagcag catcgataag tgcttgtaaa tcacgtcgtt
     6181 gagtcatagt aaatgcctca gtgcaggtaa ggtagatgtt tcttttgttt tgtatatgtt
     6241 gcgatattgt gattaaagag atgagatgta caatggcatt tcgggcagag agataacctt
     6301 gaggttatgg caaatggcga actgggcgca aaaattaaaa ctacatcacc ttgaaatgct
     6361 ggtggcgctg ggtgaacagg gcaatctaac ccatgtcgca cgaatgatga atatcaccca
     6421 acctgcgctt tcgaaatggt tgtcacaact cgaagatgag attggaatca cactttttga
     6481 gcggcacagc aaaggattac gtccgactga aggcggcaaa ttgttattgc aacatgccca
     6541 acggttaatc aatgacatgg agcgatcgca acatgaactg gcccgtttta aacaaggtgg
     6601 actggtcggc agtctgaaaa ttggctgttc tcccgtcgcc actgactgcg tatcccaggc
     6661 gattttgcat ttgcttacga agatgccaac tctacacctg aatatcgaag agaaggtgat
     6721 gaccccattg ctgcaagatc tattggccgg ttctgtcgat gtggtcgttg ggcgaattgg
     6781 cggtcgcgca ttgcagctgc cactgaatta tcaggtgctg tacaccgagc cagtatgctt
     6841 tgtcgcccgc cctgagcacc cactggcgaa atacgccacc ctcagctgga atgatcttgc
     6901 caactggcgc tggatcgtct ggccgacggg tacccccatt cgcattagta ttgataatgc
     6961 gctggtagac cacggcgtaa tgctgccaga aaacacgatt gagtcggcgt cgatgaacgt
     7021 cagtaccaac ctgttacaaa gcagcgatat gatctctatt ttgtcattac gcctggcgca
     7081 acgttatgcc agccagggac aactggtgat actcaacctg ccaaagatag aacagaaagg
     7141 tagcgtcggt gtgttctggc gcaataacga aacgccttct tatgctttaa gtcagtttct
     7201 gcacttttta cagcagaatt agtgtgctga acggggcgct cgcccgaggt tacctcccta
     7261 aaattcaact atgctgagaa ttcttaatgt gatcggtagc acgttttacg aattaattgt
     7321 atgatgaatc catctcatct ggggtgttga ttatgagtaa gacactgaac attatctggc
     7381 aatatttacg cgctttcgtc ctgatttatg cctgcctgta tgcaggcatt ttcattgctt
     7441 ccctgctacc ggtaaccatt ccgggcagca tcatcgggat gctgatcctg tttgtcctgc
     7501 tggccttgca aattcttccg gcgaaatggg tcaatccggg gtgctacgta ctgattcgct
     7561 atatggcgct attgtttgtg ccgattggcg taggcgtcat gcaatatttt gatttgctcc
     7621 gcgcacagtt tggcccggta gtggtttcct gtgcaatcag tacgctggtg gtttttctgg
     7681 tggtgagctg gagttcgcaa ctggtacacg gtgaacgtaa agtcgtaggt cagaaaggat
     7741 cagaagaatg atggcgaata tctggtggtc attaccgctg acgttgattg tcttttttgc
     7801 cgcccgcaaa ctggcggcac ggtataagtt tcccctgctt aacccgttgc tggtagcaat
     7861 ggtggtgatc attccttttt taatgctgac cggcatctct tacgacagtt actttaaagg
     7921 tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc gcgctggcct atcctttata
     7981 tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc atcaccatct gttttatcgg
     8041 cagcgtggtt gcgatggtga cggggacttc cgtggcattg ttgatgggcg cttcaccgga
     8101 aatcgccgcg tcaatcctgc cgaaatcagt caccacgcct attgcaatgg cggttggcgg
     8161 cagtattggc ggtattccgg caatcagcgc agtttgcgtg attttcgtcg gcatcctcgg
     8221 cgcggtattt ggccatacat tgcttaatgc gatgcgtatt cgtaccaaag ctgcgcgcgg
     8281 tctggcaatg gggactgcct cgcacgccct cggtacggcg cgctgcgccg agctggatta
     8341 tcaggaaggt gcatttagtt cgctagcgct ggtgttatgc gggataatta cttcgctgat
     8401 cgcaccgatc cttttcccga ttattctggc agtaatgggc taaaatttgc gatgcgtcgc
     8461 gcatttttga tgtaagtttc acgcgttgca taattaatga gattcagatc acatataaag
     8521 ccataacggg ttcgtaaact gttatcccat tacatgatta tgaggcaacg ccatgcatcc
     8581 acgttttcaa accgcttttg cccaacttgc ggataacttg caatctgcac tggaacctat
     8641 tctggcagac aagtacttcc ccgctttgtt gaccggggag caagtctcat cgctgaagag
     8701 cgcaacgggg ctggacgaag acgcgctggc attcgcacta cttccgctgg cggcggcctg
     8761 tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt gcgcgcggtg tgagcggaac
     8821 ctggtatttt ggtgccaata tggaatttat tggtgcgaca atgcagcaaa ccgttcatgc
     8881 cgaacaaagc gcgatcagcc acgcctggtt gagtggtgaa aaagcgcttg cagccatcac
     8941 cgttaactac acgccttgtg gtcactgccg tcagtttatg aatgaactga acagcggtct
     9001 ggatctgcgt attcatctgc cgggccgcga ggcacacgcg ctgcgtgact atctggcgga
     9061 tgcctttggg ccgaaagatc tggagattaa aacgctgctg atggacgagc aggatcacgg
     9121 ctatgcgctg actggtgatg cgctttctca ggcggcgatt gcggcggcaa accgttcgca
     9181 catgccttac agtaagtcgc caagcggtgt cgcgctggaa tgtaaagacg gtcgtatttt
     9241 cagcggcagc tacgctgaaa acgccgcatt caacccgact ctgccaccgt tgcagggagc
     9301 gttaattcta ttgaatctca agggttatga ttacccggat atccagcgcg cagttctggc
     9361 agagaaagcc gatgcgccgt tgattcagtg ggatgccacc tccgcaacac tgaaagctct
     9421 cggctgtcac aatatcgacc gcgtgcttct cgcttaagcc tggtgccgga tgcggcgtga
     9481 acgccttgtc cggcttgcca gccctctcct ggtgtcgaaa atcccggcaa acagtttgtc
     9541 gtttcttgcg caaaaccagc gggtaaagta gcctgatgga aattttcctt agatcgagtc
     9601 tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc tgatcggctt gctgctgttg
     9661 actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg cgccttatat ctacgacgaa
     9721 ttgcaggatc tcccctaccg ccaggtcggt gtggtgctcg gaacagcaaa atattatcgt
     9781 actggcgtaa ttaatcagta ttatcgctac cgcattcaag gagcgattaa tgcctataac
     9841 agcggtaagg taaattatct attactgagc ggcgataacg cattgcaaag ttataatgag
     9901 ccgatgacca tgcgcaaaga tttaatcgct gctggtgtcg acccatcaga tattgttctc
     9961 gattacgcag gctttcgtac gctggattcc atcgtgcgta cacgcaaagt tttcgatact
    10021 aatgatttca ttattatcac ccaacgtttc cactgtgagc gggcattatt tattgcgctg
    10081 catatgggga ttcaggctca gtgttatgcc gtaccgtcac cgaaagatat gctgtcagta
    10141 cgtattcgtg aatttgccgc ccgtttcggt gcgctggctg acctttatat ttttaaacgt
    10201 gaaccgcgtt ttttagggcc gctggtccct attccggcta tgcaccaggt gccggaagat
    10261 gcgcaggggt atcccgccgt cacacccgaa cagttacttg aattacaaaa gaaacaagga
    10321 aagtagttat ggatgtacag cagttttttg tcgttgccgt ttttttcctt atcccgatat
    10381 tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc gattgataaa cgggttaaaa
    10441 atgcaccgga accggtgtat gtctggcgag caaaaaatcc cggactcttt ttcgcttata
    10501 tggtggcata tatcggcttc ggaattttat ctatcggcat gattgtttat cttattttct
    10561 atcgttaata ttttctcttt tctgttaatt attgagaata attattactt cactcaataa
    10621 gctgcggata taacattctg aaccgcagct atttgtgaat cctttcacag tttaaatttc
    10681 cccacgcgca tagccttaat atcagcacgc aatatttatt tagcgttcgc cttaattact
    10741 catagcatta aggaagatca catgccgcaa caaaattatc tggatgaact cactccggct
    10801 tttacgccgt tactggcgat taaagaagcc tctcgctgtt tattatgtca cgacgctccc
    10861 tgtagtcagg cttgcccggc acagaccgat ccggggaaat ttattcgctc aatctacttt
    10921 cgtaatttta aaggcgctgc cgagacaatt cgcgaaaata atgccctcgg tgccgtttgt
    10981 gccagagtgt gcccgacgga gaaattatgc caaagcggtt gtacccgtgc cggtgttgat
    11041 gctcccattg atatcggccg cttacagcgt tttgttactg attttgaaca acaaaccggg
    11101 atggaaattt atcagcccgg tactaaaacg ctcggcaaag tcgcgattat tggcgcaggt
    11161 cctgccggat tgcaggccag tgtgacgcta acaaaccagg gttatgacgt cacgatttat
    11221 gagaaagaag cgcaccccgg tggctggttg cgtaacggta ttccgcaatt tcggttaccg
    11281 caatcagtgc tggatgcaga aatcgcccgt attgaaaaaa tgggcgtgac cattaagtgc
    11341 aataacgaaa tcggtaaaac actcaccctg gagcaactga aagcggaaaa ccgcgcggta
    11401 ctggtcaccg tggggttatc aagcggttcc gggctaccgc tgtttgagca tagcgacgtt
    11461 gagattgccg tcgacttcct gctacgtgca cgacaggcgc aaggcgatat cagcattcca
    11521 caaagcgcat taattatcgg cggcggtgat gtcgcgatgg acgtagccag cacgctgaaa
    11581 gttctcggct gtcaggcggt aacttgcgta gcgcgtgaag agttagatga gtttccggca
    11641 agcgaaaaag agtttgccag cgcccgggag ctgggcgttt cgatcatcga tggattcacg
    11701 ccagtagccg tggaaggcaa taaagtcacg tttaagcatg tacggttatc gggcgaactg
    11761 acgatggcgg cagataaaat tattctcgcc gtcggtcagc atgccagact ggatgacttt
    11821 gcgaagttag agccgcagcg taacaccatc aaaacacaaa attatcagac ccgcgatccg
    11881 caagtctttg ctgctggcga tattgttgag ggtgacaaaa ccgtggtcta tgccgtgaaa
    11941 accgggaaag aagccgccga ggcgattcat cactatttag agggagcttg ctcatgttaa
    12001 cgaaagatct ttcgattact ttttgcggcg tgaagtttcc caacccgttc tgcctctctt
    12061 cttcgccggt aggcaactgc tatgagatgt gtgccaaagc ctacgacaca ggttggggcg
    12121 gcgtggtgtt taaaacgatc ggctttttta tcgccaacga agtctcgccg cgttttgatc
    12181 atctggtgaa agaagatacc ggttttatcg gcttcaaaaa tatggaacag attgctgaac
    12241 atccgttgga agagaatctg gccgccctgc gtcggctgaa ggaagattac ccggacaaag
    12301 tattgatcgc ttcgatcatg ggggaaaatg agcagcaatg ggaggagctg gcgcgcctgg
    12361 tgcaagaagc tggcgcggat atgatcgagt gtaacttctc ctgtccgcaa atgacttctc
    12421 atgcgatggg tagcgatgtc gggcaaagcc cggagctggt agaaaaatat tgtcgggcag
    12481 tgaaacgggg ttccacgctg ccaatgctgg cgaagatgac gccgaatatc ggtgatatgt
    12541 gcgaagtggc gctggcggcg aagcgcggcg gcgcagatgg cattgcggcg attaacaccg
    12601 ttaaatccat caccaatatc gatcttaatc agaaaatcgg tatgccgatc gttaacggaa
    12661 aatcgagtat ttccggatat tccggtaaag cggtaaaacc gatcgccctg cgcttcattc
    12721 agcaaatgcg cacccatcca gaactgcgcg atttcccaat cagcggtatc ggcggcattg
    12781 aaacctggga ggatgcggcg gagtttttat tgctcggcgc agcaacgtta caggtcacca
    12841 ccggcattat gcagtacggc tatcgcatcg tagaagatat ggcgagcggg ttatcgcatt
    12901 atctcgccga tcagggattt gattcgttgc aggagatggt aggtctggcg aataacaata
    12961 ttgtcccggc ggaagattta gaccgcagtt atattgtcta tccccgtatc aatcttgata
    13021 aatgtgttgg ctgtggacgc tgttatattt cctgttacga cggcggtcac caggcgatgg
    13081 aatggagcga gaaaacccgc acaccgcatt gtaataccga gaaatgtgtg ggttgtctgc
    13141 tttgtggtca cgtctgcccg gtgggttgta ttgagctcgg ggaagtgaag tttaagaaag
    13201 gcgagaaaga acacccggta acgttgtaaa accagagacg catccggcat ttggttcctg
    13261 tcgccggatg cggcttgaac gccttatccg gcctacaaaa ccatacaaat cagtatgttg
    13321 agctcattgc aggccggata aaacgcgcca gcgttgcatc cggcatttgg ctcctgtcgc
    13381 cggatgcggc gtgaacgcct tatccggcct acaaaaccac acagatcatg atgttatgtg
    13441 cgtttatgac cgaatgcgga ccacaatcat ttcacttctt acgcgcgtac ttcagtgaat
    13501 ccagcgctac ggcgaagata ataatcgccc ctttgatgat gtactgccag tatgggttta
    13561 cgccgatgta cgtcaggcca tagttgatga cggtaaaaat aattaccccg gtcaccacgc
    13621 caatcaccgt ccccacaccg ccgctgaacg atacaccgcc taccacgcac gccgcgatag
    13681 catccagttc atacataaag ccgaggttgt tggtggcaga gccgatacgt ccggcttcta
    13741 acatcccgcc aaaggcatag aacacgccag acaacgcgta gatcatcagc aggttcaggc
    13801 cgacgttgac accagatact tttgccgctt ccgggttacc gccaatggca aaaatgttct
    13861 taccgaagcg ggttttgttc cacaacaccc agacgaacgc caccgcaatc aacgcgtaga
    13921 aggtgatgta agagagacgg aaactcccca gcgcgacaaa gccctgagca aaggtagaga
    13981 agccactgtc aaaaccagaa attggcgacg ccccgacaaa gtcgtaataa agcgagttga
    14041 tgccatagac gatgatcatc gtgccgagcg tggtaatgaa cggcgtcacg ttgagataag
    14101 cgataatcag accgttgatc aaaccgatca ccgcaccaat ggcacagaca atcagaataa
    14161 ccagcgcaat cggcatcgtc gccatttccg ggaacacttt gttggcgtta tccatggact
    14221 gcaataatgt cgctgccacc actgccgcca gccctacttg acgccccgca gaaaggtcgg
    14281 tcccctgggt gacaattaac cctgccacac cgagcgcgat aataatacgc accgatgact
    14341 gggtgagaat attacttaag ttcaacagac ttaaaaatgt tggggcctgg aaaataataa
    14401 tcgcaagcaa aactaaaaga acgacgtaaa taccgccctc tttcaggtaa gtaagaaaac
    14461 ttttcttatt taacgcactc atgggaagcc cctaatctta aaggtgcaaa gacgcaagac
    14521 gtagaatttc gttttgcgtt gttgtttttg tatcgacaat tccggaaacg aggccattgc
    14581 tcataaccag aatacggtct gttatcccta acaactctgg catttcagag gagataataa
    14641 taatcccctt gcctttcttc gccagttcgg caattaactg ataaatctca aacttcgccc
    14701 caacatcaat accgcgcgtc ggttcatcga gcattaatat ttctggttgc gttaacagcc
    14761 agcgcccaat aatcaccttt tgctgattac cacctgagag cgaaccaatt tgcgtccgat
    14821 gccccggtgt ttttacccgc atcgaatcaa tcacccactg ggtatcgctt ttcatccgcg
    14881 agttatccag taaaccaact ttatttttgt agttgcgaat attggaaatt aacgagttaa
    14941 aaccaatatc cagataggca taaattcccg ttgagcggcg ctcctcagtt accagtgcaa
    15001 atccgtggtt tatggcttcg ttggcattat ggttattgat ctttttgccg tgcaacgtaa
    15061 tggtgccagc cgatttctcg cgaataccaa ataacgtctc aacaatatcg gtacgtttcg
    15121 cccccaccag accggcaata ccgaggatct cccctttatg cagatcaaac gagacatcgc
    15181 gaatcgacgg ctggcgcagt gacgtcaggt tacgtacctc gaggatgact tcgcccggct
    15241 tgttttcttt gtcaggaaaa cgctggttaa gagaacgccc aaccatcatg gcgataatct
    15301 tgtccatcgt cagccctgcc agcggttcgg tggcgatcca ctgaccgtcg cgcaatacgg
    15361 taacttcatc acataactgg aagatttcct ccattttatg cgagatataa acaataccgc
    15421 agccgcgctc ttttaattta cgaataatag tgaacagatg attgacctct ttttcggtta
    15481 acgaagaagt cggttcatcc ataatcacaa ttttcgcgtt ataggaaaac gctttggcga
    15541 tttcgatcat ctgcatttgc gaaacagata atgtgccgac gcgcgcacgc ggatcgatat
    15601 caatatccag ttcatcaaaa atcgctttgg tttcgcggta cattttgtcc tgatcgacaa
    15661 acatgccttt ggtggggtat cgcccaagcc acatgttgtc catcaccgaa cgttgtaata
    15721 ccaggtttaa ctcctggtgt accatcgaaa taccattttc cagtgcttct ttggccgaat
    15781 ggaaatcgat ctctttaccc tggaataaaa tggtgccgga gtctttttta taaataccaa
    15841 acaggcattt taataatgtt gattttcctg cgccgttttc ccccattaat gcatggatag
    15901 aatgtggccg gacttttaaa ttaacgttat caagtgcctt aacaccagga aaagacttgt
    15961 tgataccgct catttccaac aagtattccc cggaagacgg agtcgttgag ctgaccatat
    16021 aattttacct tgttggccat acaataaggg cgcagtaata gactgcgccc aatcagtctt
    16081 atttcttgct gaattcagcc aggttgtctt tatctacgcc aacataaggt acgcggacca
    16141 ctttgttgtc gattttccag ttggtgccat cagccgcacc tttaccatcg gccaggtttt
    16201 tcgccagatc aaaggtcgct ttcgcctggt tgttagcatc gttcagtacg gtgcccgcca
    16261 gtgcaccgga tttcaccagc gccagcgctt ctggcagagc atcgacgcca aacaccggaa
    16321 tgctggactt gttgtgtgct ttcagtgctt ctaccgcgcc cattgccatc gcatcgttgt
    16381 tggcgataac cacttcgatt ttgttggcgt tcgggccaga cagccaggcg tccatcttat
    16441 ctttcgcctg agcggtatcc cacatagcgg tatctaactg taactgttca gttttaatgc
    16501 ctttgtcgtt cagctcttta atcacgtaag tggtacgtgc ttctgcatcc ggatggcccg
    16561 gttcaccttt cagcagtacg aattgaatct gaccgtcttt attcagatcc caaccctgat
    16621 tcgccgccca gtgtttagca atcaaatcgc cttgaataat gccggactct ttggagtcag
    16681 tgccaacgta gtaggctttg tcgtagctat ccagcgcctt acgagacggt tctttgttaa
    16741 agaataccac cggcacattt tgcccacgcg ctttctcaat caccgtaccc gcagctgccg
    16801 ggtcaaccag gttgattgcc agtgccttca cccctttcgc cagcaatacg tcgatctgat
    16861 cgttctgctt ggactggtca ttctgtgaat cattcatcag cagctgaaca tctggcgcgg
    16921 ctttcgcatc ttgctcaata gccttgcgca ctacagacat aaagttatcg tcgtacttat
    16981 agattgttac accaatgcga gtatcagcag cgtgtgcagt ggcaccgaat aacatgctgg
    17041 ccatcacagc agacagggtt aacaccttct tattcatggt atctccggtt tttcttatgc
    17101 agggtagtgc ttgagataaa tgctcggcgg ggcagtagag ttaatgaagt gttactgaac
    17161 gccgaagctc actttttaaa attcgttctt ccatgcccgg taacgctcca gaaaacggct
    17221 ttaattgttg ttatgacgca ttacctcggt gaaagtgatt attcactgtt acatacgggt
    17281 tacaacgtta aaacggtgca atcatagcta tcacattgtt aagatactgt gaaatcactc
    17341 acagattgaa agcggttaca tcgcctgatt tgttgagtta gtgatcgccg ccgcattctg
    17401 gcgtgttgcg acagaatggc gacgcactaa cgtcggcata aaacagtggc tggcacgagg
    17461 atcaatattg cctgctgccc cctgcaaggc cagttcggtg gctaatttcg ccattgaagc
    17521 aatgggataa cgcacggtcg ttaattgcgg gtcggtgtaa cgggcgatgg gaatatcatc
    17581 gaaaccgatg attgagagat gtaacggaat cgcaatgcca ttatctttta atgctgtcag
    17641 tgcgccagca gccatattgt cgttataagc aaatacagcg gtaagttgta gattgcgccc
    17701 cagcagttca accatcgccg cctcaccgcc cggcatgtcc ggcgtaccag tgccaatcca
    17761 gctttccggc ggaataatat cctgctcttt caacgcactc atccagcctg ctttacgcat
    17821 ggcgtcatct tcaatgccgt ggctggaaga aagataacca atacgttgat gaccgttatt
    17881 cagcaacatg cgcgtcgcca ttcgggcacc gctgagatta tccaggcaaa cgcaacgatg
    17941 ggtgtacccc ggcacaacgc ggttgattaa caccataccg ggaatgttat ccataaattg
    18001 cgccagttca tcgtcactca atgcttttga gtgaacaatc aacgcattac aacgctggcg
    18061 aattaacacc tcaatggcgt gacgctcttt ttccgcttca tgatagctat tgccgattag
    18121 cacgtatttc tgatgctgct gagcgaccag atccaccgct tttaccagcg cgccgaaaaa
    18181 cgcatcagaa acgtccatca ccaccacgcc aatggtgtcg ctaacctgag ttgccagcgc
    18241 ctgggcattg gcgtttggcc gataatccag ctcactcacg gctttcatta ctgcttcacg
    18301 cgtgtcggca ctgaccagcg tgctgttatt gagcacccgg gaaaccgttg ccacagagac
    18361 gccagcctga cgcgctacat cacgaatggt gatcatattc actacctgtt atgagattgc
    18421 agtaaatgac tgcttgctgc cggctatttt gtcagcactg aatacaggac ttcgtgaatc
    18481 gagtcacagc aatggaaacg gttacagccg ttttgttaac gaatgtgatc cagatcgtta
    18541 tcttgatgtt ttagatattg ccggtccggc agcacgcaaa gttaactgac gccagagcca
    18601 ttccaccggc ccctggcgga agtaacgcag ccagataaca gagaagagaa tattcgccag
    18661 ccataccgga ataacaaacg ccagcaattc cagacggtca aactgcataa acagaccgag
    18721 gtggtaaaaa agcgtggtac aaatcagcgt ttgcaataga tagttggtca gcgccatccg
    18781 tccgacgcag gcgatcgcca gcaccagctt aaagcggctc aattgcggcc agaagccata
    18841 aaacagcgac gcatagccaa tcgcctgaaa cggcgcactc agttcccgcg gcatttgaag
    18901 taagaaggca caccagcgat acgcccagtc cagctgccat tgcagggcga tggcaggaag
    18961 gttaatgatc accccaatcg ccactagcac aaaaccagta cgacgatagt gacgtaagct
    19021 gaactgccct ttcagccagc cactgcgcat caatgcggca ccaatgagca tcatcccagc
    19081 cagttgccag ccatactgtg cgcccagtgc caataaactg ttcccaacac catcggcacg
    19141 attactgatc gcttcaacgc cgccgtgaag cttccagtat ttttcatata aaatagccga
    19201 tgcatccggc gtccaggcgc ggctagtctg gctatcggaa atcaacccca ataacagcaa
    19261 aacgccaagc cccaccagat aaagcatgac gccggtatta aaaaggcttt ttaccgatgg
    19321 cgcatcgcgc accagccgcc agcagattaa gcccaccagc ccgtaagcca gcagaatatc
    19381 gccgtcccag aacaataaac cgtgaataaa gcccagcaag actaacagcg ttaaccgcga
    19441 ctggatccag cgtctgccac ggggcagcaa catttgcagg ccagcaccaa acagcagcgc
    19501 aaaaagcgtg aggaatttca cctggccgat gagatcgaga aatgcccagg tccatgcatc
    19561 ctgcggcgta atagcgccgt accaggcggg attaagataa gccgcctttg gtagcccaaa
    19621 ggcgctgatg tttaatagca ggatccccag aatggcaacg ccgcgaacaa agtcgagcgt
    19681 gacgttgcgc tccatggttc ctgcctttaa atcagttgtg atgacgcaca gcgcgcagaa
    19741 actcgtggcg cgtattctga ctggatttga acaatccacc aagagaggtc gttgtcgtgg
    19801 cactggttgc atcgcggatg ccacgcgcct tcacgcaata atgcaccgcg tcgatagaga
    19861 cagccacgtt attggtgccc agcagcgttt gtagcgcaat gagaatttgc tgcgtcagac
    19921 gttcctgcac ctgcggacgc tgggcaaaga actgcacaat acggttaatt tttgacagac
    19981 cgatcaccga atctttcggg atataagcca ccgtcgcttt gccatcgatg gtaacaaaat
    20041 ggtgttcaca ggtactggtc agagtgatat cgcgcacggt gaccatttca tcgaccttca
    20101 ttttgttttc aatgagggtg attttcggga agttggcgta atccagaccg gagaaaattt
    20161 catcgacata cattttagcg atgcgatgcg gcgtttccat caaactgtca tcagccaggt
    20221 cgagattcag cagctgcatg atttcggtca tatgaccagc aataaggctt ttgcgcgttt
    20281 cgttatccat ttcatgcacg ggcgggcgca gcggtgtttc cagtcctcgc gcaactaacg
    20341 cttcatgaac cagggccgct tctttactga gtgatggcat ttatgatttc tcctgcaggt
    20401 gtgacgcctc cgccctgcgt gggggcaaag ttattaagct gatttacagc ctgattattg
    20461 tgcgtgaggc ggcgcacata atccagtatt cacagcgata attattgtaa ttgccgctgc
    20521 ctttcatcag cagatgttaa aacatcgtta tgcaaatacg gaagtgaaag ttactcacag
    20581 cacattgaat aaacggtatg atgaagaaat tgcaaacaac acaacaagga gccacgcatg
    20641 gaaatgctcg aagagcaccg ctgttttgaa ggctggcagc aacgctggcg acacgactcc
    20701 agtaccttaa actgcccgat gacgttcagt atctttctcc caccccctcg tgatcacacc
    20761 ccgccaccag tgctgtactg gctttccgga ttaacctgca atgacgagaa cttcaccacc
    20821 aaggcgggtg cccagcgcgt ggcggcggaa ctggggattg tactggttat gccagacacc
    20881 agcccgcgcg gcgaaaaggt tgccaacgac gatggctacg atttaggcca gggcgcaggc
    20941 ttttatctta atgccacgca accgccgtgg gcgacgcatt accggatgta tgattatctg
    21001 cgtgatgaat taccggcgct ggttcagtcg caatttaatg tcagcgaccg ctgcgctatt
    21061 agcggtcatt caatgggtgg tcacggtgcg ctgattatgg cgctgaaaaa cccgggcaaa
    21121 tacaccagcg tttcggcctt tgcgccaatt gtgaatccgt gcagcgtccc gtggggaatc
    21181 aaagcgttta gccgctattt aggtgaggac aaaaatgcat ggctggaatg ggacagttgc
    21241 gcgctgatgt atgccagtaa cgcgcaggat gcgatcccga cgcttatcga tcagggtgat
    21301 aacgatcagt ttcttgctga ccaactgcaa cctgcagtac ttgcagaagc cgcgcgccag
    21361 aaagcatggc cgatgacgct gcgtattcag ccgggatatg atcacagtta ctacttcatc
    21421 gcctctttta tagaggatca cctgcgcttc catgcgcagt atttactgaa gtgaaagtcc
    21481 gcccggttcg ccgggcatct tctcatcaga agcgataatc cactgccata aagtaacgac
    21541 gtccgtcttc gttatagctg tagtcgtcac gactgagatc cttgtcgcca aggttcagca
    21601 cgcctgcgcg cagtttgacg tctttagtca cctgccaggc cgcgccggta ttccagatgg
    21661 tataaccgcc cggtgtttta gccgtcgcgc tgtcggctcg tttctgcccg gtatagtgac
    21721 cagaaacata gaatgaccag tcttccagcg ccagcggttt ccagtccagc gtaccgttag
    21781 cggtatggaa cggcagatcg gatagcggtt tgttttcgcc gttgctgaca tcacgaccat
    21841 cgttgtaggt gtagttgagt gacagtttcc attcatcgtt gaacggaatt ttcagctcgg
    21901 tttccacgcc ctgaatacgt gctttgttaa cgttgtagta gctaaatacc ggcacaccgt
    21961 tcgctgtaaa accaacaaag ttttggtagc ccggtgcagc attgacgtca gacgtacggc
    22021 tgatgctgat acgatctttc acatcgttac ggaaaacggt aacgctggat tcaacccctt
    22081 ccagccagcc ttcttcaccc atgtagtaaa gccccagctc ccaactttcg ctggtttctg
    22141 gtttcagatc cgggctaccc acaatcttac atgcgccacg gcaggaattg ctcgtccagt
    22201 cagggctaag ttgcaacaga gaaggtgctt taaatgccgt cgcccagccc cctttcaccg
    22261 ttacggtgtc ggtggcgtta taaaccaggt aggcacgcgg actccagtgt tcaccgtagg
    22321 tttcgtgatc gtccatacgc acgccggtcg tcagcgccag cggctcgaag atccgccatt
    22381 catcttccac aaacagcgcg tactggctgg cagacgtttt ggagctggtt cccccggtca
    22441 ggttcaccgc atcgctaagt ttgtcgtgac gccattcacc gccaaccgtg agaaactgat
    22501 taatcgccgt cagcggcaac gtgtatttgc cgtcgaccgt attgctttcg gaagttatcg
    22561 ggctgctgtt gccagggttt ttgttctcga ctttctcacc gtagtatttc agttcgctgg
    22621 tgccgtaatc ccaacgccca ttatggctga cggagtagtt ctggcgttcc aggcggtttt
    22681 tgtccagcga gtcggaatca cgatcctgac ggtcgaaacc gtatccggca gtaaaatcgt
    22741 gattttgatt cggtgtccag gcaaattcga cattgccgtc gcggctggag aatccttcaa
    22801 tacgcggcgt ttctccggta tcggtggtcg ttgagttttg cgggtcatcc ttttcacgtt
    22861 ttgccaggct gccgtaagct ttcattccca gcacaccatc aattaatggt ccactggtaa
    22921 agaactgacc gttataggta tcaccgcgat cgcgatgttc ctgaatggtg gtatcgacgg
    22981 taacggtacc cgaccatttc tgaccgattt ttttggtgat gatattcact acaccgccga
    23041 gcgcatcgga accgtacagc gacgacatcg ggccacggac cacttcaata cgttcgatgg
    23101 aatcgaccgg gatccagttc agatcgaaat cattgtggcg gaagacggca ttgcgggagt
    23161 taacgcgttt accgtcgaca agaatcaggg tgtagctgct gtccagacca cgaatactaa
    23221 cgcccttacg gttatcccct tcgttcgtca gttgtacgcc aggcacttct ttgaggacat
    23281 ccttcagatt ctgtaccggt tttcgctgca ggtcttcctg ggtaatgacg ctgatactgg
    23341 cgggtgcatc tttaaggttt tgttcaacgg aagatgcagt gacaaccatc gtttcgccat
    23401 cgtcatcgac cgctaacaca ggccatgcac aagaaatagc ggacaaacac agcccgaccc
    23461 gtacgaaagg gttcaaccta aacattccat atctccatga ggtaactacg aaaataaaat
    23521 gggttatcgc tcacatcttc ttcacgtccc cttgcgtacg gcagcatcgc ggtggactta
    23581 tttgtatgca ggtgatcatc ccagaaagcc ggaccagcct ctgatttgtc agcctttttt
    23641 gattgcggcg taacgataat gcaaacgata acaattatca atccaaatgt taaattttat
    23701 atccgcatga tttgtcaggg aaatggaatt gaaaaagccc tctcggttga gagggcttag
    23761 caaggaaggg agaaacttat ttcttatcgt tctgcgggaa cttcatttcg ctgtagcgta
    23821 cgaagtgggt tcctttaatc agcttgtagc cgaaccaaat aatcaggaac agcgggatac
    23881 caatatacgt tgccgctacg ccgccccagt caatggtatc tttcaggaac gcttcgtagt
    23941 tctggcccaa agtgataatc agacacagaa tgaatgcgaa gatcggcccc aatgggaaga
    24001 aacctgaacg gtacggcaga tcgttaatgt cgtgtccctg caatacgtaa ccgcgacgga
    24061 agcgatagtg gctaatggca atccccagcc aggcgataaa accggtcatc ccggaggtat
    24121 tcagcagcca caggtatacc gtctggttgc caaacatgga ggtcaggaag cacagaccgg
    24181 caatcaccgt cgtcgcatac agcgcattac gcggcacgcc accacgcgac agtttagcga
    24241 aaatgcgcgg cgctttaccg tcacacgcca gggtgtacag catacgagta gacgcataca
    24301 taccggagtt acccgctgac agcaccgccg tcagaataac tgcgttcatc accgccgcag
    24361 cagagagcag acccgcgtgc tggaacacca gggtgaacgg actaacgctg atgtctttaa
    24421 catcgttacg cagcaggctc ggatcggtgt acggaataat caggctgata atcaggatcg
    24481 cgaacacata gaacaacagg attcgccaga acacctgacg taccgcgcgt ggaatgtttt
    24541 tccccggatc ttcggactcg cctgcagcaa taccgatcag ctcggttccc tggaaagaga
    24601 agccgacaat catagctacg ccgatcatcg ccgcaaaacc accagcaaac ggcgcttcgc
    24661 cgattgtcca gttgctccag cccgcaggct gcgcgccttt gaagataccg ataatcatca
    24721 gcacgccaac gatgataaag acaataactg tcgtgacttt gatcagtgag aaccagtatt
    24781 ccgcttcacc aaagccgcga acagagatgt agttcagcag gaagataacg ccgaggaaca
    24841 acgcactcca gatccagccc ggtgtatccg ggaaccacca gctcatgacc agctgagctg
    24901 caaccaggtc aacggcaata gtcaccgccc agttgtacca gtagttccag cccagcgcga
    24961 agccaaagcc ctcttcaaca tagttctgac cgtaagtggc aaacgaaccg gaaaccggca
    25021 tatatgcagc cagttcaccg agactggtca tcaggaagta aaccatcagg ccaatcaaca
    25081 tatacgagag caatgccccg cccggacctg cctgagaaat cgttgcgcca gaggcaacaa
    25141 aaagacctgt accgatggaa ccgccaatgg caatcatcgt caggtgacgc gcctttaatt
    25201 cacggcgtaa gcccggcgct tctgtggttt tagtttcgga aaccatacaa aaatgctatc
    25261 catctaaaaa atgaggcgcg attgtagcag acgattggca atccttccgg cagaaataca
    25321 cggttataag acaccttcat gatcgcccag ggattataag taaagcagcc aatctctttt
    25381 ctggcgaatg catgctgaag gcgctgcgtc acgaaatcga cacagcacca gcatgttctt
    25441 gtacagcaac ttaacgcggc acatttgcgg gatcgcaata gtccagaaag cgccgtagcg
    25501 cgttggaaag gtgtttttgc cgatgatgta tacgccacaa cgtacgcatc aggcgcggca
    25561 gagggaccgc aacttcactt aatgtgcctg cctgcaattg atcttcaatc acacgtcgcg
    25621 acaggcaact aattcccaac ccatgacgca ccgcatgttt gattgcctcg gagttaccta
    25681 attccatcgc catctcaaac ttcggtaaat gtgacagcaa cagataatcg acaatctccc
    25741 gcgtgccgga accgcgttca cgcaggatcc acggcgcagc ggccagctgt tctaaggtga
    25801 ccggaccacg ggccaacggc gaagtcggcg cggcgaaaac caccagctcg tcttccagcc
    25861 acggttcaga aatgatttca gtgctgtggc acggtccttc aataaagcca atatcaacgc
    25921 ggaaatccag caccgcttgc atcacgtcct ggctattccc aacgctaagt tcaatcggca
    25981 actgcggata atcatggcga taacgggcga taactgcagg cagaatgtag ttaccgatgg
    26041 tactactggc atagatacga atcgcgccgt tgtcttcgcg aaacagttgt tcgatttcaa
    26101 ccgcctgttc aagcaatgcc aacgcacgcg gatagagcag ccgcccgtgt tcattaacaa
    26161 ccagtctttt ccccacgcga tcaaacagtt gcacgccaag ctgcccttcc aggtcggtca
    26221 aggctgcgct cactgctgat tgcgacaacg ccagcatcac cgacgcttgg gtggttgatc
    26281 cacttttcaa tacttctgca aaaacttcca actgccggag ggtgatgtgc atagtcgctt
    26341 accacttata aagattaatt ataaatatat aatcaatttt atttttaaac cagttagtcg
    26401 taaccttatg cccggttaaa ggagagggtt atgacgaata tcaccttaca gaaacaacat
    26461 cgtacactgt ggcattttat tccggggtta gccctgagtg cagttatcac cggggtcgcc
    26521 ctgtggggtg gttccattcc cgcggttgcg ggtgccgggt ttagtgccct caccctcgca
    26581 atcttgttgg ggatggtttt aggcaacacc atctatccgc acatctggaa aagctgtgac
    26641 ggtggtgtgc tgtttgccaa gcaatattta ttacgtctgg gtatcattct ttatggcttc
    26701 cgtctgacgt tctcgcaaat tgccgatgtc ggtatcagtg ggatcatcat tgacgtgctg
    26761 acgctgtcca gtaccttcct gctggcttgc ttcctggggc agaaagtgtt tggtctggat
    26821 aagcacacca gctggttgat cggtgccggt agcagtatct gtggtgctgc cgcggtactg
    26881 gcgactgagc cggtagtgaa agcggaagcc agtaaagtaa ccgtggctgt tgcaaccgtt
    26941 gttatcttcg ggaccgtcgc gattttcctc tacccggcga tatatccgct gatgtctcaa
    27001 tggtttagtc cggaaacttt cggtatctac attggttcta ctgtgcacga agtggcgcag
    27061 gtggtggcgg caggtcatgc catcagcccg gatgcggaaa acgcagcagt tatttccaaa
    27121 atgctgcgcg tgatgatgct ggctccgttc ctcatcctgc tggcggcgcg tgttaaacag
    27181 ctgtctgggg cgaacagcgg cgagaaaagc aaaatcacta ttccgtggtt tgccatcttg
    27241 ttcatcgtag ttgccatctt taactcgttc cacctgttac cgcagagcgt ggtgaacatg
    27301 ctggtaacgc tggatacctt cctgctggca atggcgatgg cggcgctggg tctgaccact
    27361 cacgtcagcg cgctgaaaaa agctggggcg aaacctctgc tgatggcact ggtactgttt
    27421 gcctggctga ttgttggtgg tggtgcgatt aactatgtga ttcaaagcgt catcgcataa
    27481 accactacat cttgctcctg ttaacccgct atcattaccg ttttcctcca gcgggtttaa
    27541 caggagtcct cgcatgaaat acattggagc gcacgttagt gctgctggcg gtctggcaaa
    27601 tgccgcaatt cgcgccgccg aaatcgacgc aaccgcgttt gccttgttca ccaaaaacca
    27661 acgtcagtgg cgtgccgcac cgctcacgac gcaaaccatc gatgaattca aagccgcctg
    27721 tgaaaaatat cactacacat cggcgcaaat tcttccccac gacagttatc tgattaacct
    27781 cggacatccg gtcactgaag ctctggaaaa atcgcgcgat gcctttatag atgaaatgca
    27841 gcgttgcgaa cagctggggc tttctttgct caacttccac cctggcagcc atctgatgca
    27901 gatttcagaa gaggattgcc ttgcgcgtat tgccgaatcc atcaacattg cgctggataa
    27961 aactcaaggt gtgacagcgg tgatagaaaa caccgccggt cagggcagta acttagggtt
    28021 taaattcgaa catctcgcgg cgattatcga cggcgtggaa gataaatccc gcgtcggcgt
    28081 ctgcattgat acctgccatg ctttcgctgc cgggtatgat ttgcgtactc cagccgaatg
    28141 cgagaaaaca ttcgcggatt ttgcccgtac tgtcggcttt aagtatctgc gcgggatgca
    28201 ccttaacgat gcgaaaagca cctttggcag ccgcgttgac cgccatcata gcctcggtga
    28261 aggcaatatc ggtcatgatg cgttccgctg gatcatgcag gacgaccgtt tcgacggcat
    28321 tccgctgatc ctcgaaacca tcaacccgga tatctgggca gaagagatcg cctggctgaa
    28381 agcgcaacaa actgaaaaag cggtagcctg aagatgaata accgggaaaa ggagatcctt
    28441 gcaattttac ggcgtaaccc gctgattcag cagaacgaaa ttgcggacat gctgcaaatc
    28501 agccgttcgc gcgttgcggc gcatattatg gatttaatgc gcaaaggccg gattaaaggc
    28561 aaaggttaca ttctcaccga gcaggaatac tgcgtagtgg tggggacaat caatatggat
    28621 attcgcggga tggcggatat ccgttacccg caatcggctt ctcatcccgg tacaattcat
    28681 tgctcagcgg gcggcgtggg acgcaacatc gcccacaatc tggcgctgtt aggccgtgac
    28741 gtccatttgc tttcagtgat tggcgatgac ttttatggcg aaatgctcct ggaagaaacg
    28801 cgccgcgccg gcgtgaatgt ctccggctgc gttcgtttac atggtcaaag cacatcgacg
    28861 tatctggcaa ttgccaatcg agacgatgaa accgtgctgg cgattaacga tacccatctg
    28921 ctggaacagt tatcgccgca attattgaac gggtcgcgcg atttacttcg tcatgcgggc
    28981 gtggtactgg ctgattgcaa cctgacagcc gaggcgctgg aatgggtctt taccctcgct
    29041 ggtgagatcc cggtgtttgt cgataccgtt tcagaattca aagcgggcaa aatcaaacac
    29101 tggctggcgc atattcacac cctgaaaccc actttaccgg agctggaaat tttatgggga
    29161 caggcgatca ccagcgatgc tgaccgtaat gctgcagtga atgcgttgca tcagcaaggt
    29221 gttcagcaac tgtttgttta tttgcccgat gagtcagttt attgcagcga aaaggatgga
    29281 gaacaatttt tgctgaccgc accagcgcat acgacagtag acagttttgg tgctgacgat
    29341 ggttttatgg cgggcctggt atatagcttt ctggaaggaa acaatttccg tgacagcgcc
    29401 cgttttgcga tggcctgcgc ggcaatttca cgcgccagcg gcagcttaaa caaccctacc
    29461 ctgtctgccg ataacgcgct ttcattagtg ccaatggtgt aacaatgttg ccggattcga
    29521 cgcttgaccg catcttatcc ggctcataaa tcgcacacta cgctaaacca ataaagaacc
    29581 cggcaatggt ggcactcatc aggttagaaa gtgtcgccgc cgccagcgcg cgtaaaccaa
    29641 gctgggcgat ttccggcgca cggtgtggcg caaccgcaga aaacgccccc accaccaccc
    29701 cgatagaacc aaagttagcg aaaccgcaca acgcgaagga aataatcgcc acggttttag
    29761 catccagagt gccagccgtt tgcagatagg gtgagaaatt gagataagcg acaaattcat
    29821 ttattgccag tttctgtcca atcaaactcc cggcaagatt cgcatcactc cagtcaacac
    29881 ccattaccca cgccagtggg gccagcagat aacctacaat ggactccagc gaggcatgtt
    29941 caaaaccaaa ccagccgcca acgccgccga taataccgtt aattaacgca atgatggcga
    30001 cgaatgccat cactactgtc gccacacctg cggcgatttt cagcccggtc attgcccctg
    30061 tcgcggcggc ttcaataatg ctttttggcg gtgtttcggt gaaagagagg ttattaaaag
    30121 aaacctgcga agattccgta gccgggctta gcaggcgggc aaacaagatc cccccgggga
    30181 tcgccattaa cgatgccgcc agcaaatatt ccacaggcac gcccagtgcg gcataaccaa
    30241 tcattgtcga accagcaatc gaggccatgc cgctacaaat cgctgtaaat aattcattgc
    30301 gattcagccg atcgataaag ggcttcacaa tcgccggaat ttcgttttgc ccgaggaaaa
    30361 tggtagtgac cgcgacgaat gactcaatct tgctgatatt taatgctttc tggaatatac
    30421 cgccgagaat gcgaattaaa atccccatca caccgatgta gtagagaata ctcaccagtg
    30481 cagtgacgaa gataattgcc ggtaataccc tgaaaccaaa gataaatcct gcgccatcaa
    30541 ataaggtgtc cattttcggt ccgaccagag aaccgaagat aaatgcgcta cccgcgtcgc
    30601 tgtacgccat cactttatgc acaccaaaag cgactttttc agcgacccaa cgccctggcg
    30661 gtaaccaaag cataatgccg ccaatcacga cctgtaacac taacgccgcg ccaacggtac
    30721 gcaggctgat cttcttcttg tttactgaca gtaaaaacgc aatcgtcagc aataccacca
    30781 ttcccagaac acttctcatg acatccatag tgattatctc ttcatgtcat attgggcgac
    30841 ggctttctgc ccatcagaaa tagccaggcc tgcatcttgc gataccggcc cggcttgtta
    30901 tcaacactta atgggttttg atgtagccgc gcacgcactc ttcgactaag ccccagaacc
    30961 agtcgctatc aatagtgatg ccgactttgg tattagcggg cttgccaaga acgcccagct
    31021 cgtcgcagac ggtacgccca taacaagggc cactgttgac gtccacttcg acgtacatct
    31081 cctgggtttt aatgccatca gggttaatca gataaccgat gcaggtggcg tcgtgcaccg
    31141 ggccgccagc caggccgtag ttttcgaact gggttttgag agtgaagttc atgatgtcgc
    31201 tgaacagctc tccggcaggg ccgcctgccc tttccattcg tgcaatcacg tccggggtgc
    31261 aaacggtctg gttggtgaga tcgaggccca tcatcactaa tggaacgcca gaggtgaaca
    31321 ccacgcgtgc ggcttccggg tcagcaaaga tgttgaactc ggcagatggc gtgaagttgc
    31381 ctgtaccgta agcgccgccc attagcacaa tttcacggat tttgggcagg atcgcgggtt
    31441 gcatacgcat tgccaccgcg atatttgaaa gcggaccaac cggcaccaga gtgatatcgc
    31501 catcgcttgc catcagggta tcaatgatgt atttcaccgc atgagtgctt tctgcctggc
    31561 gggtcagcgg ctcgaatacc gggccatcca gtccggtatc accgtgaata ttatcggcaa
    31621 cgatttgttg ccgcataatg ggttgtggca tccccgcata aaccggaaca ttaatctcca
    31681 gtttctggca aacattcagg ccattaatta atgttttatc aagcgtctga ttacccgcta
    31741 caatagtgat gcctaataaa tctattgccg gatgtttcgc cgccatcatt atagcaatag
    31801 catcatcatg acccggatca caatccagaa taatttttct cttttccatt gtttatttcc
    31861 tctgtttcca gttgcgttat tttttctaca gcaaagaaat ttcgatcggg ccgatgattg
    31921 aatcttaaca acagcgtacg tatgctaaat atgagaaatc tcatagcgga taaacatcgt
    31981 gaaagaaatc cacaataatg atcttaagca gcaattgatg agtgaatccg cgtttaagga
    32041 ttgcttttta acggatgttt cagccgatac gcggctgttt cattttttag cgcgtgacta
    32101 cattgtgcag gaagggcaac agccgtcctg gctgttttac ctgacgcgag gccgcgccag
    32161 gctttacgcc acgctagcta atggtcgcgt gtcgctgatc gatttctttg ccgccccctg
    32221 ttttattggt gagattgagt taatcgataa agaccatgaa ccgcgtgcgg tacaggctat
    32281 tgaagagtgt tggtgccttg cgctccctat gaaacattac cgtccgctgt tattaaacga
    32341 caccctattt ttacgaaaac tttgcgtcac cttaagtcat aaaaattatc gtaatattgt
    32401 ttctttaact cagaatcaat catttccgtt agtgaatcgc ctggcagcat ttatattact
    32461 ctcacaagaa ggtgatcttt atcacgaaaa gcatacgcaa gcggcagagt atttaggcgt
    32521 ttcttatcga catcttttat atgttctcgc gcaattcatt cacgacggtt tattaattaa
    32581 aagcaagaaa ggatatctaa ttaaaaacag aaagcagttg tcagggctgg cactggagat
    32641 ggacccggag aataaattct ccgggatgat gcagtaaaaa ttatttgcaa tagcgcgatt
    32701 gccggatgca acgcttgatg cgttttatcc ggtctacaaa ttgagcatta cgccagacca
    32761 ataaagaacc cggcaatggt cgcgctcatc aggttggaaa gcgttgctgc tgccagcgcc
    32821 cgtaaaccaa gctgagcgat ttccggtgcg cgttttggcg aaatagccga aaatgcccca
    32881 acgacaacac cgatagaacc aaagttagca aaaccacaaa gcgcaaagga gataatcgca
    32941 atggttttca cttccagcgt gccgcccgtt tgcaggtatg gggagaaact caggtaagcg
    33001 acgaattcgt taatcgccag tttctgccca atcaggctac ccgcaaggtt ggcatcactc
    33061 cagtccacac ccatgatcca cgccagcggt gccagcacat agccaaaaat actttccaga
    33121 gaagcattgg cgaagccaaa ccagccgcca attccgccga taatgccgtt gatcagcgca
    33181 ataattgcca caaatgccat taccaccgtc gccacaccag cggcgatttt cagcccggtc
    33241 atcgcaccgc tcgccgctgc ttcgataaag ctttttggcg gcgtttcgct gaacgacaga
    33301 ttttcaaacg tgacctgcga aagctcggtt gctggactaa gaatacgtgc aaacaaaata
    33361 ccgccgggga tcgccatcag cgatgccgcc aacaggtagt caattggtac gcccattccg
    33421 gcataaccaa tcatcatcga accagcaatg gacgccatcc cgctacaaat agccgtaaac
    33481 aactcgtttc gattcatgcg atcgataaac ggtttaacga tcgccgggat ctcattttgc
    33541 ccgaggaaaa tagtggtgac tgcgacaaaa gattcgattt tgctgatgtt gagggctttc
    33601 tggaaaatgc tgccaaggat gcgaatcagc agccccatca cgccaatgta gtacagcaga
    33661 ctaatgagcg cagtgacgaa gataatcgcc ggaagtacgc gaaaggcgaa gataaaaccc
    33721 gcaccgtcaa agaggacatc cattttcggc ccgaccagcg aaccaaaaat gaaggcgcta
    33781 ccagcatcac tgtaagacat cactttatga acgcctaatg ccgcctgttc tacagcccac
    33841 tttcccggcg gaaaatagag catgatgcca ccaagagcaa tttgcagcag tagtgcggct
    33901 gcaaccgtac gcaaactgat gctcttttta ttcactgaca acagaaatgc tattgccagt
    33961 aacaccacca tccccacaac acttctcatt atgtccataa tgattttccc ttcatgccag
    34021 taaacccggc gtcagcgcca ggtttcggta tgtttgatga gtacgggcga cggctttctg
    34081 cccgtcagaa attaacccgc gagacgctga tattctttgg caatttcgct cgccagaatg
    34141 gcgttgttga acacaagctg gatgttggat ttcaggctgt caccgccggt cagctcagca
    34201 acgcgcgcca gcaggaatgg cgtactttct ttaccaataa caccctgcgc ttcagcttca
    34261 gctaccgcct gatcgatcac cgcattgata gtgtgttctg gcatcgcaaa ctgttccggg
    34321 atcgggttcg ctaccacgag gccaccgttc agcccgcttt gccatttcac cgccattgca
    34381 cgggcaattt cactggcgct gtcgagacga atgctgacgt caaacgggct ggtacggcag
    34441 aaaaacgcag gcagcgcttt agtctgatag ccaattaacg gcacaccgaa ggtttctaaa
    34501 tactcagtgg ttaatcctaa atcgagaata gatttcgccc cggcacaaac aacggtgaca
    34561 ttagtatttg ccagttcttg caaatcggca gaaatatcga aggtatgttc cgcccctcga
    34621 tgaacaccac caattccgcc ggtggcaaat actttaattc cggcaagcgc cgcaataatc
    34681 atcgttgaag ccacggtggt tgcaccattt tttcctgcgg caacgacaaa aggtaaatcg
    34741 cgacgactaa ctttagtcac gttatgccct tcacgaccca gtaattcaat ttcttctttg
    34801 cttaaaccca ctttcatcac gccgccaata atggcgatag tggcaggtac tgcgccctgt
    34861 ttacgaatag tttcttctac ttcaattgcg gtctgggcat tttgtgggaa cggcatcccg
    34921 tgagaaataa tggtcgattc cagcgccaca accggttttt tgttttttaa agcgtcctgc
    34981 acttccgggg aaatttgtaa taattcaggg gaaattttta attcagacat tctgcgttct
    35041 ccactaacga tataacgttg gcaatcgata aatcggggtt attggtgtat tcacaggaga
    35101 gcgccattga cgaacatccc tgtgcgaaac gaacagattc ggcaaacggc attccgtcta
    35161 cccaacacga agcaagtccc gccatcatgg catcgcccgc tccggtaaca ttaataacat
    35221 tggttttgat cggcgcagac cagccacttt caccgctgat atcgctgtaa taaacgccgt
    35281 cgccgcccat gctcaatacc agtcggttca ggccatgttg atggaaccag gcagcaactt
    35341 ttgccacatc ttcacgccct gacagcgcaa tcccactcag ggtttccgct tcaaggcggt
    35401 ttggcttgag agtatggatt tgacttaatc gctcgcgtac tttgacgcat ttccacgcgg
    35461 aaaccggatc gacaaatacg ggtacgttgg cagcattatc cagaatccat gccagtgcct
    35521 cttcactgat attacagtcc gcgacaatga cctttgccct ctgaataaat tcacggtgct
    35581 gtgcgagata ttcagctgta atagcgttgc taatattcat gtcatttata gcaaccagca
    35641 tttcaccggt attatcgagt aatgataaat agctcgacgt attttctccc ggcacaatca
    35701 ggcatttatc gacataaacg ccagattgat tggtttgcgt tagcagcgat tggccataaa
    35761 aatcactgcc tacggcgctc agtagccagg ctttattacc cagcaacgcc aggttttgtg
    35821 caatattgcg ccctacccca ccaggcgtaa attttatttt acctggattt gaatccgcat
    35881 aatttaatga ttcatgtgaa tatccggcga catcaatatt cgccgagcct ataattacga
    35941 cataatcctt ttcgcgcata gcacgtcctc tggtaaaaaa ttaccaaccc tgtcaatcca
    36001 caaggatata gatactgcaa agacggttta aacatgtgtt catatttaaa catgtgctca
    36061 tagtatgcat gttttacgta aagtaaattg ccgccagaga ggcaaagcgg gcagatttga
    36121 gcaggctcac aaaatgcgat taaatgcctg aattacaaaa gcgaggaatt tgtgacggga
    36181 tgcacagaac atgaacacta aaaaagggca gaaaagaatc tgcccttgag gaaataacct
    36241 tagtgtttga tgaacgtttc aggtttctac tgaaagataa aaccatttaa attcataaaa
    36301 ttacattcac aaaccggtca ttctctttgc gctcatccaa tgatacgggg cagtaaattt
    36361 ctgccctgta acacaccttt tattacgcgg ctttcgctac tgcgtccact tccggacgtt
    36421 tcaggaaggc ataggccaaa cccgccacca gcgtaccggc aataattgct accaggtaac
    36481 ccagtaccgg cgtaatagcg ccagggatca gcagaacaaa cagaccaccg tgcggtgcca
    36541 tcagtttcgc accaatcgcc attgagattg cgccagtcag cgccccacca acgatacaac
    36601 acggcagcac acgcatcgga tcacgagcag caaaaggaat tgcaccttcc gaaataaagc
    36661 acagaccaag aaccagagcg gctttgccac cttcctgctg cgctttgtca aatttgcgac
    36721 gcgccaccat tgttgccaga cccattgcca gcggtggcac catacctgcc gccataatcg
    36781 ccgccatcgg gccataggtt tgagtactca gcagacccac accgaatgcg tacgctgctt
    36841 tatttaccgg accgcccatg tcagtacaca tcatgccacc gaggatcgcc cccagcagaa
    36901 ccgcattcgc agtccccatg gtctgcagcc agtgagtcaa cccttcgaga atgccagcaa
    36961 ccggtttacc gatcaggtag atcatcgcca gaccgaccac cagactggaa attagcggaa
    37021 tgatcaggat cggtttcagc gcctccatac tctgtggcag tttcagttgc gtactgatta
    37081 acttcgcaat gtaaccagcc aggaagcccg caataatacc gccaatgaag ccagaaccgg
    37141 tgctgaccgc cagcataccg ccaatcagac ccggcgtgag acccggacga tcggcaatgg
    37201 agaaggcaat ataacctgcc agtaccggca ccatcagcgc aaaggctgaa ccaccaccaa
    37261 tctgcatcag cgccgctgcc aacgtacccg gctctttaaa cgcttcgata ccaaaagcaa
    37321 aagaaagcgc gatacacaga ccacctgcaa cgaccatcgg cagcatgtaa gagacgcccg
    37381 tcagcaagtg acggtatgcg cctgcactct cgttcttacc ttcagaggtc gccgtttgag
    37441 ctttgcccgc cggttcatac ggcgttgctt cagcaaccgc tttatccagt tcctgcgcgg
    37501 ttttcttcag cgccagaccg gtagaggtgc gatacatcgg tttaccagca aatttcgcca
    37561 gatccacttc gatatctgcc gccacaatca ccagatccgc tgcggctact tcttcgggag
    37621 tgattgcatt acccgcgcca acagaaccac gggtttcaac tttcacccac cagccacgtt
    37681 ttttcgcttc ggtttcaatg gcttcagccg ccataaaggt gtgtgctacg ccagtcgggc
    37741 aagcagtcac cgcaactacg cgtttcggac cgctggcggc aaccggtgct gtcgcagcga
    37801 ccggcgcagt gtaaggtttc gcatggcctt tggcttcact caggaacagc tcagggtgcg
    37861 caactgcccg ggaaatatcg cccagccaga catttttacc gttcagcgcg ctgtcattcg
    37921 ggatggaatc accgagaaca atcgccattt ctgcgtcgtt cggattgtcg atgatttcca
    37981 gttttgcttt tcgcgccgcc gcgcccagca gggtcttcgc catataggcg cgtgcctgac
    38041 cgagattagc gtcaataatc agcagcgttt tcattatgcc tctcctgctg tcagttaaaa
    38101 ggttgtaagt cgacgcgcgc catcattgcg gccaactgcg gacgatcggt aatacccaca
    38161 ttgctttgac ttaccgccag ggctgcaaca gcagtcgcca gacgcagtgt gtgttcactg
    38221 gattcacgca ttagcaagcc ataaatcagg ccaccaacca tagaatcccc tgcgccaacg
    38281 gtgcttacga catcgactga cggtggtttg gcgatccatt cgccggaggc attaacccaa
    38341 agcgcgcctt cggcacccag tgaaataacg acatgcgcaa taccttgttc acgcagcgca
    38401 tgcgcagctt caatcacatc tttcatttca ggcagtttac ggcctgccca gatttccagc
    38461 tcgcggcggt taggtttcac cagccacggt gccgctttca aacctgctac taacgcttca
    38521 cggctactat caaagataat gcaagggcac tgactacgca ggcgagtcat ccagtcggtg
    38581 aacgcttccg ggctgacgcc tgacggtaag cttccgctga cacagaccat atcgaactga
    38641 ccgagccagc tcagagaatc agtcacaaag cgttcccagt cggcgggggt gacttcaaaa
    38701 cccgagaagt tgaagtcggt cacttcgccg tctttttccg tcagcttaac gttaattcgg
    38761 gtgcgcccct gtacaacctg gaaacggttg gcaatgccca gctcgctgaa cagttgctga
    38821 aaaccatcct gattgtcttt accgaggaag ccgccaacgg tgacatcaat tcccaggtct
    38881 tttaatactt tggccacgtt gatgccttta cccgccgcat gcagaccggt ggttttcacc
    38941 aggttcactt cgccgcgttc aatttccggg cagaaaccaa caaggtcata agccggatta
    39001 agggtgatag tagcaacacg tctgctcatt atgcgccctc cccaagacca gcagcgatag
    39061 cgtcgccgat tgctttcagc gcctgttcag catctgcacc ctgggcggta aagcgtaggc
    39121 gatgaccttt cttaacgcca agtgccacaa ctttcatcag actacgtccg tttgccggtt
    39181 tgccggtgcc atcaaggttt gtcacggtaa tatcactgtt aaattgttta atggtgttga
    39241 ccagcatggt acctggacga gcatgcaggc cgtgttcatt gcgcaccaca aactccgcgc
    39301 ttaacacgtc gtcggtcggc gcatcatcgc tggtcagcag cgccagcaac gttgccgcat
    39361 ccgctttcag caagcggtca gctttattgt cgagcaacaa atcagcgaga cgcttaagaa
    39421 ccgcaatggg ctgatcgtca ttcatcgcca cactcaccag catggctgcc gtttcgccgt
    39481 ccacatcaaa agcatttgcc gcacggctta ccgcaattgc gctacgcaga ttgccttcgg
    39541 cgctatcgct cagccagata ccctgtccga ggttcagcgg ttgttcattg atggctttgg
    39601 tgacgaaagt ggcgtcaact gcccccgcct ctttcagacg cgcagcgttc agcgcctgaa
    39661 gagtcagcag atcgctggcg acgatatcca gcgtcagcat ttcgttgtcg agcttcagct
    39721 gctcactctg cttttcgccc atcagcaatg cgcgaagttc ttctgctgtt gttgctgact
    39781 tcagttgttc agcaacggaa tcatcgctca gtacgtgggt cagctggcgt agcaggccca
    39841 gatgctcatc cgagctggca gcaataccga tcgctacgta cgctacctga ccgtcacccc
    39901 aggtgacgcc ttccgggaac tgaaatacct gaacgccggt tttcagcacc tgatcgcggg
    39961 tgtcggtagt gccgtgtgga atagcaatac cattgccgag gaacgttgag gtttgctgtt
    40021 cgcgcgccag catgccattg acgtagcctt ctgctacatt accggcctgc accagcgccg
    40081 cagcgacctg gcgaatcgcc tcttctttgt ctccggcctt ttcgcccgga tggatgtcct
    40141 gtacggataa ctggaacata gttctcctct cttgctgaat tgaaacgatt cagcttctat
    40201 gagaaaaaaa gcgccaacct ggcttagggt taaagacaag atcgcgctga aacgtttcaa
    40261 gaaagcataa tacttctgtt tcagcacgca aggaaagccg taaagttatg agcaaaagtt
    40321 tgatgtgctg cacaattttt tgtcattttt cctaattgct gatgggaaaa tccgttatca
    40381 gcaatttcat ttcagcagcg tcagcagcca atcagcaaca gtgaaatggc attttgatta
    40441 ttcgtggcgc ttttgatttc gctgtctgtt tattttctga caagcagcgt aaactccgcg
    40501 tcttcctctt ccagtgatcg accagcatgc ataactcccc cgcagtctcc agcgcgaaat
    40561 cgtttgacct gacctcgacg gcgtttttaa tcgttgcctt tctcaccggt attgcgggcg
    40621 ctctgcaaac cccgacactc agtatttttc ttaccgatga agtacatgcc cgtccggcga
    40681 tggtgggatt cttctttacc ggcagcgctg tcattggggt tctggtgagt cagtttctcg
    40741 ccgggcgctc tgataagcgc ggcgatcgca aatcgctgat tgtcttttgc tgcctgttag
    40801 gcgtgctggc ctgcaccctt tttgcctgga atcgcaacta ctttgttttg ctattcgttg
    40861 gcgtctttct tagcagcttt ggctcgaccg ctaacccgca aatgtttgcc cttgcccgtg
    40921 aacatgccga caaaaccgga cgtgaggcgg tgatgttcag ctctttttta cgcgctcagg
    40981 tttcactggc atgggtcatt ggcccaccgc tggcttatgc cttagcgatg ggtttcagct
    41041 ttacggtaat gtatctgagc gcagcggtag catttattgt ttgcggcgtg atggtgtggc
    41101 tgtttttacc gtcgatgcaa aaagagcttc cgctggcgac cggcacggtt gaagcgccgc
    41161 gccgtaaccg tcgcgatacg ctgctgctgt ttgtcatttg tacattgatg tggggctcga
    41221 acagcctgta catcatcaac atgccgctat ttattatcaa cgaactccat cttcccgaga
    41281 aactggccgg tgtgatgatg gggaccgccg ccgggctgga aatcccgacc atgttgattg
    41341 ccggatattt cgccaaacgt ctgggtaagc gtttcttaat gcgcgttgct gccgtgggtg
    41401 gcgtctgttt ttacgcagga atgctgatgg cgcattctcc tgtcattctg ttgggcttgc
    41461 agctgctaaa tgctattttt attggcattc tgggcggtac cgggatgctc tattttcagg
    41521 atctgatgcc cggtcaggca ggttcagcca ccacgctcta taccaacacg tcgcgcgtgg
    41581 gctggatcat cgcgggatca gtggcgggca tcgtcgccga gatctggaat tatcacgctg
    41641 tgttctggtt tgcgatggtg atgattatcg ccactctgtt ttgcttactg cggattaaag
    41701 atgtttaagg cgcggtcagc atttccagat cgataagcca ggtcattgcc tgctgacgcg
    41761 agttaccgca catttcagcg ctggcttgta agcctgcaca gactttcggc cgcagtggcg
    41821 aggtgaaaat tttacagcgt tgctgttcat caagctgaat gcagggcgta ttggcgggct
    41881 tgccatctgg cataccggga atagggctgg aaattgaagg ggcagtgcaa cacgccccgc
    41941 aacccggacg gcattccata cgttgttctc ttaaggttaa ccaccggatt gatgagcgcg
    42001 cacagtacca ccttttttgc accagcaaaa gtgcgaatac cacttgcccg aaaggctcgt
    42061 cgcgagtact ttgtcgcgat atttttgaca ttttcgacta caggaatttt tcgatgccaa
    42121 gagcgaacga aattaaaaaa ggtatggtac tgaattacaa cggcaaactg ctgctggtta
    42181 aggacattga tattcagtcg cccactgccc gcggtgccgc tacgctgtac aaaatgcgtt
    42241 tttctgatgt ccgtaccggg ctgaaagtag aagagcgttt caaaggagat gatatcgttg
    42301 acaccgtgac gctgacccgc cgttacgttg acttctccta tgtcgatggc aacgaatatg
    42361 tctttatgga taaagaagac tataccccgt ataccttcac caaagatcag attgaagaag
    42421 agttgctgtt tatgccagag ggcggcatgc cggacatgca ggtgctgacc tgggacggtc
    42481 aactgctggc gcttgagctg ccgcagaccg ttgatctgga aatcgttgaa accgcaccag
    42541 gcatcaaagg ggcatctgcc agcgcccgta acaaaccggc gacattgagc actggtctgg
    42601 tgattcaggt accagaatac ttaagcccgg gcgaaaaaat tcgtatccat atcgaagaac
    42661 gccgttatat ggggcgtgct gactaacttc agccgcatgc agaaaaggga tagctcaggc
    42721 tgtccctttt ttaatttatt ataccaatct tctattttgc gcttcctgaa caccttacgc
    42781 cctcaatttt cactcgttga ttgatcgccc tcacacttca tcgcattaac aatccagacc
    42841 aatttcaatt gctgtcatat aactttacac tgtcattgtt aattaatcgt tactaagaca
    42901 tgactcctat gaatacaatc gcctccgtta cgctcccgca tcatgtacac gctccacgct
    42961 acgatcgcca gcagttgcaa tcacgtatcg ttcattttgg ctttggagcc tttcaccgcg
    43021 cacatcaggc gttactgacc gatcgtgtgc tgaatgccca gggcggcgac tgggggatct
    43081 gtgaaatcag cttgttcagc ggtgatcaac tgatgagcca gctccgcgca cagaaccatt
    43141 tatataccgt gctggagaaa ggggcggacg gcaatcaggc gataattgtc ggtgccgttc
    43201 acgaatgcct gaatgcaaaa ctggattcct tagcggcaat tattgagaaa ttttgcgagc
    43261 cacaggtggc aattgtttcc ctgacgatta ccgaaaaagg ctattgtatt gacccggcca
    43321 ccggtgcact cgacaccagc aatccgcgga ttattcacga tctacaaacc cctgaagaac
    43381 ctcactccgc accgggtatt ctcgtcgaag cactgaaacg ccgccgtgag cgcggcctta
    43441 caccgtttac cgtgctctcc tgcgacaata ttcccgacaa tggccatgtg gtgaaaaacg
    43501 cggtgctggg aatggcagaa aaacgttcgc cagaactcgc cgggtggata aaagaacacg
    43561 tcagttttcc gggaaccatg gtcgaccgca ttgttccggc tgcaaccgac gaatcactgg
    43621 cagaaatcag ccagcatctg ggggtaaatg atccctgcgc gattagctgc gaaccgttta
    43681 tccagtgggt ggtggaagat aacttcgtcg ctgggcgtcc tgcctgggaa gtcgcaggtg
    43741 tacaaatggt gaatgatgtc ctgccatggg aagagatgaa actgcggatg cttaatggca
    43801 gccactcttt tctcgcttat ctgggttacc tctcaggatt cgcccatatc agtgattgca
    43861 tgcaggatcg cgcatttcgc catgccgcca gaacattaat gctggatgag caagcgccga
    43921 cactgcggat taaagatgtc gatttaacac aatatgcgga taagttaatt gcacgttttg
    43981 ctaatccggc gctgaaacat aagacctggc aaatcgcgat ggatggcagc cagaaattac
    44041 cgcaacgcat gctggcaggt attcgcatac atctggggcg cgaaacggac tggtcgttgc
    44101 tggcattagg cgttgcaggc tggatgcgtt acgtcagcgg cgttgatgat gccggaaatg
    44161 ccattgatgt tcgcgatccg cttagcgata aaattcgcga tcttgttgcg ggcagcatca
    44221 gtgagcaacg cgtcaccgcc ctgctttccc tgcgtgaagt tttcggtgat gatctgccag
    44281 ataacccgca ttttgtgcag gccatcgaac aagcccggca acaaatcgca caattcggcg
    44341 cgcatcaggc gctattaaac accctcaaaa tttaacgatt tctgcggtta aagcggatga
    44401 agctcgcctt cgtccgctct ccccttctct tttctgccgt ttttagccag gattaacgct
    44461 cagttaactt accagagtat tgcggagctg ttgtgaccag gaccaacctc atcaccggtt
    44521 ttctcggcag cgggaaaacc acgtcgattc ttcatctgtt agcccataaa gatcccaacg
    44581 aaaaatgggc ggtactggtt aatgaatttg gggaagtcgg aattgatggt gctttgctcg
    44641 ccgatagcgg cgcactgctg aaagagatcc ccggcggctg catgtgctgc gttaatggtt
    44701 tacccatgca ggtagggttg aataccttac tgcgtcaggg aaaaccagac cgcttgttga
    44761 tagagccgac tgggctgggc catccgaaac agatcctcga tctgttaacc gcaccagtct
    44821 atgaaccgtg gatagatctg cgcgccacgt tgtgcattct cgatccacgc ctgctgctgg
    44881 acgaaaaaag cgccagcaat gaaaacttcc gtgaccagct ggctgccgca gacatcattg
    44941 tcgccaataa atccgaccgt gcgacacctg aaagcgagca agcgctacag cgctggtggc
    45001 agcaaaatgg tggcgatcgg caattaattc acagtgagca tgggaaagtt gccggtcatc
    45061 ttctggattt gccgcgtcac aatttagccg agttgcccgc cagcgccgcg cattctcatc
    45121 agcatgtcgt gaaaaaaggg ttagcagcgt taagcctgcc agagcatcaa cgctggcgtc
    45181 gcagtctgaa cagtggacaa ggacatcagg cctgcggctg gatattcgac gctgatacgg
    45241 tattcgacac cattggcatt ctggaatggg cgcgacttgc accggtggaa cgcgtcaaag
    45301 gcgtgctgcg tattcccgaa gggctggtgc gaatcaaccg tcagggcgat gacctgcaca
    45361 ttgaaacgca aaacgttgcg ccaccggaca gccgtattga gctgatttcc agcagcgaag
    45421 ctgactggaa tgccttgcag agcgcgctgt tgaagcttcg tttagcgact accgcgtaag
    45481 gttgcctgcg tttttcagta agataattag agaaaatatg attaaaaatt tgccgcaaat
    45541 agtgttgttg aatattgtcg gcctcgcgct gtttctttcc tggtatattc ccgttaatca
    45601 tggattctgg ttgccgattg atgcggatat tttttatttc tttaatcaga aactggtcga
    45661 aagtaaggcc tttttgtggc tggttgcatt gaccaacaat cgcgctttcg acggttgttc
    45721 actgctggca atgggaatgt tgatgctgag tttctggctg aaagaaaacg ccccaggcag
    45781 acgacgtatc gtgattattg gtctggtcat gctattaact gcagtggtat taaaccagct
    45841 gggtcaggca ttaattcctg taaaacgggc cagcccaaca ttgactttta ccgatattaa
    45901 ccgcgtcagc gaactgctct ctgtccccac gaaagatgcc tcacgagata gctttcccgg
    45961 cgatcacggc atgatgctgc ttattttttc ggcattcatg tggcgttatt tcggcaaagt
    46021 tgcaggtctt atcgccctta ttatttttgt ggtttttgcg tttcccagag taatgattgg
    46081 cgcacactgg tttactgaca tcattgtcgg ttcgatgacc gtgatattga tcggtttgcc
    46141 ctgggtgttg ctgacgccat taagtgatcg attaatcacc ttttttgaca aatcactacc
    46201 gggaaaaaac aaacatttcc aaaacaaata actcacagta cttaacatca tcaggtttat
    46261 ttttatagtg aggataatcc tgatgatgcg caccgtgctt tcatccatcg aaagcaaaaa
    46321 tcattctcta agtaaatgaa tggattgcat gcatttcact caatcgtact ttaattgacc
    46381 aatcccgctt attaactttc tgtatcactt tttcttataa aaaatcatgt aaaaccgctc
    46441 gccaagaccg caccaatcgg gtaatctcga actcgttttg cctcggcggt agattatcct
    46501 cacagcatat aattttgtgc gttagtccac agatttggcc ttaaggaatt gtttcaacat
    46561 gccctggtaa ttagtctcgt gtcgcttggc atttttttta taacgatatt tgtcgttaag
    46621 gacttcaagg gaaaacaaac aacatggtca aatctcaacc gattttgaga tatatcttgc
    46681 gcgggatccc cgcgattgca gtagcggttc tgctttctgc atgtagtgca aataacaccg
    46741 caaagaatat gcatcctgag acacgtgcag tgggtagtga aacatcatca ctgcaagctt
    46801 ctcaggatga atttgaaaac ctggttcgta atgtcgacgt aaaatcgcga attatggatc
    46861 agtatgctga ctggaaaggc gtacgttatc gtctgggcgg cagcactaaa aaaggtatcg
    46921 attgttctgg tttcgtacag cgtacattcc gtgagcaatt tggcttagaa cttccgcgtt
    46981 cgacttacga acagcaggaa atgggtaaat ctgtttcccg cagtaatttg cgtacgggtg
    47041 atttagttct gttccgtgcc ggttcaacgg gacgccatgt cggtatttat atcggcaaca
    47101 atcagtttgt ccatgcttcc accagcagtg gtgttattat ttccagcatg aatgaaccgt
    47161 actggaagaa gcgttacaac gaagcacgcc gggttctcag ccgcagctaa taaaccgttt
    47221 ggatgcaatc ccttggctat cctgacgagt taactgaaag cactgtttag gcagtgcttt
    47281 tttgttttca ttcatcagag aaaatgatgt ttccgcgtct tgatccaggc tatagtccgg
    47341 tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat taacggaatc atgttcatac
    47401 gcgctcccaa ttctggacgt aagctcctgc ttacctgcat tgttgcaggc gtaatgattg
    47461 cgatactggt gagctgcctt cagtttttag tggcctggca taagcacgaa gtcaaatacg
    47521 acacactgat taccgacgta caaaagtatc tcgataccta ttttgccgac ctgaaatcca
    47581 ctactgaccg gctccagccg ctgaccttag atacatgcaa gcaagctaac cccgaactga
    47641 ccgcccgcgc ggcgtttagc atgaatgtcc gaacgtttgt gctggtgaaa gataaaaaaa
    47701 cattctgttc atctgcgacc ggtgagatgg acattccact caatgaattg attcctgcgc
    47761 tcgacattaa taaaaatgtc gatatggcga tcttacccgg cacgccgatg gtgccgaaca
    47821 aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa aaatagcggc gtctttgccg
    47881 ctctgaatct caacctgacg ccttctctct tttatagctc acggcaggaa gattacgatg
    47941 gcgtcgccct cattattggc aatactgcgc tatctacctt ttcttcacgt ttgatgaacg
    48001 ttaacgaatt aaccgacatg ccagtccgtg aaactaaaat tgcgggcatt cctctgaccg
    48061 ttcggcttta tgcagatgac tggacatgga acgatgtgtg gtacgcattt ttactgggcg
    48121 gcatgagtgg aactgtcgtt ggcctgctct gctattacct gatgagcgta cgtatgcgcc
    48181 ccggcagaga aatcatgacc gccatcaagc gcgaacaatt ttacgtggcg tatcaaccgg
    48241 tggtggatac acaagctttg cgagtaacgg gcctggaagt actgctacgc tggcggcatc
    48301 ctgtcgcggg agaaattccc ccggatgcct tcattaactt tgccgaatcg caaaagatga
    48361 ttgtgccgct gactcagcac ctgtttgagt taattgcccg cgatgccgca gaattagaaa
    48421 aagtgctgcc ggtaggcgtc aaatttggca ttaacattgc gccggaccat ctgcacagcg
    48481 aaagctttaa agcaaatatc cagaaactgc tcacttccct acccgcacac catttccaga
    48541 ttgtgctgga aattaccgag cgcgatatgc tgaaagagca agaagccaca caactcttcg
    48601 cctggctgca ctcggtcggc gtagaaattg ctattgatga cttcggcacc gggcacagcg
    48661 cgcttatcta tcttgagcgt tttacgctcg attatctgaa aattgaccgt ggatttatca
    48721 acgccatcgg tacggaaacg atcacttcac ccgtacttga cgcggtgctg acgctggcga
    48781 aacgcctcaa tatgctgacg gttgctgagg gggtcgaaac gccggaacag gcgcgatggc
    48841 taagcgaacg cggcgttaat ttcatgcaag gctactggat tagtcgcccg ttaccgctgg
    48901 acgattttgt ccgctggctg aagaaaccgt atacgccgca gtggtaaggt gtgcttacgt
    48961 cccttattat tcatagtgaa agcatgccgg attgcggcta atgatgagta aaaggaaatc
    49021 cgttgcagat gattgtgcgc atactgctgc tgtttatcgc tctgttcacc tttggtgcgc
    49081 aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct gggcgaaccc cggtacgcgt
    49141 ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc aaaaggtggg cagataacgt
    49201 tgtcagccct cggtaccttc gataatttca accgctatgc actgcgcggc aacccgggcg
    49261 cacgcaccga gcagctgtac gacacgctat ttacgacttc cgatgacgaa ccaggcagtt
    49321 attacccgct gattgctgaa agcgcacgct atgctgacga ttattcctgg gtggaggtcg
    49381 ctattaatcc tcgcgcccgt tttcatgatg gttcgcccat tactgcccgc gatgtagagt
    49441 ttacttttca aaaatttatg actgaaggcg tgccgcaatt tcgtctggtc tacaaaggca
    49501 ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga gttagctaaa cccggcaaag
    49561 aagatatgtt gagtctgttt tcgctgccgg tatttccaga aaagtactgg aaggatcaca
    49621 aacttagcga tccgctcgcc acgcctccgc ttgccagtgg tccgtaccga attacgtcct
    49681 ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga ttactgggca gcaaacttac
    49741 cggtaaaccg tggacgctgg aatttcgaca ccattcgcta cgattattac ctcgatgata
    49801 atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt gcgtatggaa aacgacgcca
    49861 aaaactgggc cacacgctat agcggtaaaa atttcgataa aaaatacatc atcaaagatg
    49921 agcaaaagaa cgaatcagcc caggatacgc gctggctggc gtttaatatc caacgtccgg
    49981 tattcagcga tcgccgggtc cgggaagcaa tcaccctcgc ctttgacttt gaatggatga
    50041 acaaggcgtt gttttacaat gcctggagtc gcacgaacag ttattttcag aataccgaat
    50101 acgcggccag aaattacccc gacgccgcgg agctggtgct tctggcacca atgaaaaaag
    50161 atttaccgcc agaagtcttc acacaaatct accagccgcc ggtatcgaaa ggcgatggct
    50221 acgatcgtga caacctgtta aaagccgaca aacttctcaa cgaagcgggc tgggtgctga
    50281 aaggtcagca acgcgttaat gccactacgg gtcagccgct cagctttgaa ttattgcttc
    50341 ctgcaagcag caatagtcag tgggtattgc cgttccagca cagcctgcaa cggctgggta
    50401 tcaacatgga cattcgcaag gtggatcact cgcaaatcac caaccgcatg cgcagtcgcg
    50461 actatgacat gatgccgcgc ctatggcggg cgatgccgtg gcccagttcc gatttacaga
    50521 tttcctggtc atcggaatat atcaattcca cttataatgc ccccggcgtg caaagcccgg
    50581 ttatcgactc gctgatcaac caaattattg ccgcgcaggg aaataaagaa aaattactgc
    50641 cgttagggcg agcactggat cgcgtattaa cgtggaatta ttacatgctg ccaatgtggt
    50701 acatggcgga agaccgtctc gcctggtggg ataaattctc ccaacccgct gtgcgccctg
    50761 tttacagtct gggtatcgat acctggtggt atgatgttaa taaagcggcc aaactgccgt
    50821 cagccagaca acagggagag tagatgggcg cttatctgat tcgccgtctg ttgctggtga
    50881 tcccaacgtt atgggcgatt atcactatca actttttcat cgtgcaaatt gcgcctggcg
    50941 gtccggtcga ccaggccatc gccgccattg agtttggtaa tgccggagta ttacccggcg
    51001 caggcggtga aggcgttcgc gccagccatg cgcaaacggg cgtcggtaat atcagcgaca
    51061 gtaattaccg tggcggacgc ggattagatc cagaagtgat cgccgagatc actcatcgct
    51121 acggtttcga taagccgatc cacgaacgtt acttcaaaat gctctgggac tacatccgct
    51181 ttgattttgg cgatagcctg tttcgcagcg cctcggtgct gacgctgatt aaagacagtc
    51241 tgccggtttc catcaccctc ggattgtgga gcacgctgat tatctatctg gtgtcgattc
    51301 cgttaggcat tcgcaaagct gtttataatg ggagccgctt tgacgtctgg agtagcgcat
    51361 ttatcatcat cggctacgcc attccggcct ttttgtttgc catcctgctg attgtcttct
    51421 tcgcaggcgg cagctatttc gacctgttcc ctctacgcgg cctggtttcc gctaactttg
    51481 attcgctgcc gtggtatcag aaaatcaccg attatctgtg gcatatcacg ctgccggtgc
    51541 tggcgacggt gattggtggc tttgcggcgc tgactatgct gacaaaaaac tcattccttg
    51601 atgaagtacg caagcaatac gtggtgaccg cgcgtgcgaa aggggtaagt gaaaaaaata
    51661 ttctctggaa acatgtgttc cgcaacgcca tgctgctggt gattgccggt tttccggcga
    51721 cgtttatcag catgtttttt accggctcgc tgctgattga ggtgatgttt tcactcaatg
    51781 gtcttggctt actgggctac gaagcgaccg tctcgcgcga ttatcctgta atgtttggta
    51841 ccttgtatat tttcaccctg attggcctgc tgctgaatat tgtcagtgat atcagctata
    51901 cgctggtcga tccgcgtata gattttgagg gacgttaatg tcgcgactca accccgtcaa
    51961 tcaggcccgt tgggcgcgtt ttcgccataa ccgtcgcggc tactggtcgt tatggatttt
    52021 cctcgttttg tttggtttga gtttgtgttc tgaacttatc gccaacgata aaccgttgct
    52081 ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa aactacagcg aaagcgattt
    52141 tggcggcccg ctggcaagtc aggctgatta tcaggacccg tggctgaaac aacggctgga
    52201 aaataatggc tgggtactgt gggcaccgat tcgctttggt gctaccagta tcaactttgc
    52261 taccgataag cccttccctt ctcccccctc ccggcaaaac tggctgggaa cggatgccaa
    52321 cggcggcgat gtgctggcac gtattctcta tggcacgcgg atctcggttc tgtttggcct
    52381 gatgctgact ctctgttcca gcgtgatggg cgtgctggcg ggggcgctac aaggctatta
    52441 cggcggtaag gtcgatctct gggggcagcg ctttattgaa gtatggtcgg ggatgccaac
    52501 gctgtttttg attattctgc tttccagcgt tgtgcaacct aacttctggt ggctgctggc
    52561 aattactgtc ttatttggct ggatgagtct ggtcggcgtg gtgcgggcgg agtttttacg
    52621 tacccgcaat ttcactacat ccgcgcggca caggcgcttg gcgtcagcga tcgcagtatt
    52681 attctgcgtc atatgttgcc gaatgcaatg gtcgctaccc tcaccttttt accgtttatt
    52741 ttatgtagtt cgatcaccac cctgacctca ctcgatttcc tcggcttcgg tctgccgctc
    52801 ggttcaccgt cactcggtga actgctgtta caagggaaaa ataaccttca ggctccgtgg
    52861 cttgggatca ccgccttctt gtcggtggcg atattattgt ctttgctgat ctttattggt
    52921 gaagccgtcc gcgacgcatt tgatcctaat aaggcggtgt agcatgacgc aaactctgtt
    52981 agcgattgaa gatttgtcgg tgggttttcg ccatcagcaa accgtacgta cagtagtcaa
    53041 tgatgtttca ctacagattg aggctggcga aacgctggcg ctggtgggtg agtcaggttc
    53101 aggcaaaagc gttaccgcgc tgtcaatttt acgcctgctc ccttccccgc cggttgaata
    53161 tctctccggc gatattcgtt ttcatggcga atcgctgctt catgccagcg accaaacgtt
    53221 gcgcggtgta cgcggtaata agatcgccat gatttttcaa gagccgatgg tgtcgttaaa
    53281 tccattgcat accctggaaa aacagcttta tgaagtgctt tcactccacc gcgggatgcg
    53341 tcgggaagcg gctcgtggcg aaattcttaa ctgccttgat cgcgttggta tccgccaggc
    53401 ggcaaaacgg ctgacagatt atccgcatca gctctccggc ggcgaacgcc agcgggtgat
    53461 gattgcgatg gcgctgttaa cgcgaccgga attattaatt gccgatgaac cgaccaccgc
    53521 actggacgtc tctgtccagg cgcagatttt acagctgttg cgcgaactgc aaggcgagtt
    53581 gaatatgggc atgctgttta ttactcataa cctcagcatt gtcagaaaac tggcccaccg
    53641 cgtggcggta atgcaaaacg gtcgctgtgt cgagcaaaat tacgccgcta cgctatttgc
    53701 ctcacccact catccttaca cacaaaagct actcaacagt gaaccgtcag gcgatccagt
    53761 gccgttgcca gaacctgcct caacgttgct ggatgttgaa cagcttcagg ttgccttccc
    53821 cattcgcaaa gggattttga agcgcattgt ggatcataat gtggtggtga aaaacatcag
    53881 ttttacgcta cgagcgggtg aaacactggg tttagtgggc gagtccggtt ccgggaaaag
    53941 cacgacggga ctggcgctgc tgcgactgat taattctcag ggcagcatca tctttgacgg
    54001 tcagccactg caaaatttaa atcgccgcca gctgttacct attcgtcatc gcattcaggt
    54061 ggtatttcag gatccaaact cctcgctcaa cccacgactc aacgttttgc agattattga
    54121 ggaaggctta cgggttcacc agccgacgct ttctgccgca caacgcgaac aacaagtgat
    54181 agacgtgatg catgaagtgg gattagatcc tgaaacacgc caccgttatc cggcggagtt
    54241 ctctggtggt cagcgacaac gtattgcgat tgccagggca ttaattctta agccctcgct
    54301 gatcatactt gatgaaccga catcatcact cgacaaaacg gtacaggcgc aaatattgac
    54361 gctattgaaa tcattgcaac aaaagcatca actggcctat ttgtttatca gccacgattt
    54421 gcacgttgtc cgcgcgttat gtcatcaggt tatcgtactg cgacaagggg aagtagtgga
    54481 acaaggaccg tgcgcgcgcg tgtttgccac accgcagcag gagtatacgc gtcagctact
    54541 ggcgttgagc tgacgcttaa aaaggattgt agtttgaaaa gggttcggca attgccacac
    54601 caaaattttt caatcggcat gttgcagcaa actcgtcctg gcgatttaca aacaggcacg
    54661 gctcaccttc acactccagc accgaacatg gaacttcgat gtcgcctaac gcctggctta
    54721 gtttatacat caccgaccat gcattatcac catccgggct aagcaattta agcgccacca
    54781 ggctgttgtc accgcacggt ccattttgcg gaatcggttc aaccttcgaa cctgcgaaac
    54841 cagcagacca gcgatagttc tgcggcagtc gatggacgat tgagagttgt aatgaagtca
    54901 cttattttcc ccggaagcac atttacttca caatttgttt acatcaattt taacacatca
    54961 tcaacaatcc gtctttatac agatcgtaaa gattgatgcc tcgcctgcgt ggcctctatg
    55021 gctctgattt aagtaatatc tgcgggcgtg gtcacgtttg cggggctata tgtacccgtt
    55081 tctgcgatct aactcaacct ttttaactac aatgatgtga ctttttacat aaattgattt
    55141 tacataaaat aaacatatat cgggggaatg atagatttgt ggttgacatg catcaacaaa
    55201 ggaagccttt tagcttcctc gttgtgcaat agatcaccgt tttttcggcc gactggcgta
    55261 cagacagaag agaatggagc tggttgcgca gaatgcaatt gaccaaatca tcggccatgc
    55321 agagttaaag gtcgcaagag aaagcaatgc gccaacaatt gcccctatgc caaaacggaa
    55381 ggttcctgcc agcgaagatg ccgttcccgc catatgggga aactcatcaa gaatgaccgc
    55441 catcgcattg gatgacacca tcgacacgca gcccacaaac gccgcaacgc caaccaccag
    55501 cgaccaaaat cccagaccca gcagcgcact gatgaccatc cacgctgcca taataaattg
    55561 tatccacaac cccgagcgga acatatttaa cgcgccaata cggcggacga agcggctatt
    55621 aaagatggtc atcacgaaca ggaaaacaat gtttagtgca aagtaataac caaagttttc
    55681 cggcgcgacg tggttaattt caatgtagac aaacggtccg gcgcttaaga acgagaacat
    55741 cccggcaaag ctgaaaccac tggccagcat ataactcagg acacgcttat gacggaacag
    55801 cgccgcaaag ttaccaatag tggtacgaat gtgaaaaggc tggcgacgct ccggtggtaa
    55861 ggtttctttg atcagaaaga agatcattgc cgaagccaga atcgccgcca cggcgaggat
    55921 ccagaagatg taatgccagc tcagccacac cagcacccag ccgccaacta tcggtgccat
    55981 cagcggtgca atggtcgtca ccagcatgac aaacgacatc atccgcgaga actcttcttt
    56041 cgggtagata tcacgcatca aagcgttgat aaccacgctg gccgcagccg ccgccagccc
    56101 gtggaagaaa cgcatcacaa tcagctgatc gatggtttgt gccaacgcac acgccaccgc
    56161 ggcggcggca aacaccagcg taccgccaag aaccaccggc ttacgcccga agctgtctgc
    56221 catcggcccg tagattaact gccccaacgc aaagcccaga atataagtac tgagggtcat
    56281 ctgcgtactg cccgccggta cgccaaactg cgctgaaatt accggtagcg cgggcagata
    56341 catatcaatc gacagcggca tcaacatggc cagcaggcca aggataaaaa caatagcaaa
    56401 cgacgaatgc tgtcgggtgg tcacaacggg ctcctgaaag tcattgaaaa gttagacgac
    56461 gctggcaatt tcttcttcag ttaacggacg atattcaccg ggggctaaat cagcatccag
    56521 cgtaatgccg ccaatacgtt cacgatgcag ctcaaccaca tggttaccca cggcggcgaa
    56581 catgcgtttc acctgatgat aacgcccttc gctgatggtc agacgaacct gcgttggggt
    56641 aatcacttca agcaccgctg gcttagtgag atctttttcg ttatgcagct gcacgccttt
    56701 agcaaattgc tctgccgtat cgtcagctac gggtgattcc agcgtcacca gataggtctt
    56761 ctcgcaatga tggcgcggag aagtaatgcg gtgcgaccac tgaccatcat ctgtcattag
    56821 taccagaccg gtggtatcga tatccagccg ccctgcggcg tgcagtttcc acgccactgg
    56881 ttcatctaga aaatagagca ccgttgggtg atcagggtcg tccgtggagc aaacatagcc
    56941 ctgaggctta ttgagcatga agtaacgtgg accgtgttgc tgcgccagcg ggttgccatc
    57001 gtaagcgaca tcatgttcag gaagcagttt gaacgctgca ttacggacga tttcgccatc
    57061 gacggtgaca cgattgccgc ggatttcacg cccggcaata gcacggctaa cgccgagttg
    57121 ctgtgcgata aatttatcaa gtcgcatgtg tgtgattttg cctgtaaaaa cggaggtcgg
    57181 gcattgaccc gaaaatctga actgttgtct gcccagtata gcggtctaat aacttccctc
    57241 aagggaaaaa gaaccgtggc atactattag cagaataatc tacctacgcg agaccatgat
    57301 ttttacactc cgcccatatc agcaagaagc cgtggatgcc acgctcaacc attttcgtcg
    57361 tcataaaacc cctgccgtta tcgtgctgcc caccggtgca ggtaaaagcc tggtgatagc
    57421 ggaacttgcg cggctggctc gtggtcgcgt gctggtgctg gcacacgtta aagaactggt
    57481 ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa gccgatattt ttgccgccgg
    57541 gctaaagcgc aaagagagcc acggtaaagt ggtatttggc agcgtgcagt cggtcgcccg
    57601 taatcttgat gcctttcagg gtgaattttc gctgttgatt gtcgatgaat gtcaccgtat
    57661 tggtgacgat gaagagagcc agtatcagca aatcctcact cacctgacaa aagtgaatcc
    57721 ccacttacgc ctgctggggc tgactgccac gccttttcga ctgggcaaag gctggatcta
    57781 ccagttccat tatcacggca tggtacgcgg cgatgagaaa gcccttttcc gtgactgcat
    57841 ttatgaactg ccgctgcgtt atatgattaa acacggctat ctgacgccgc cagaacgact
    57901 ggatatgcca gtagtgcaat acgatttcag ccgcttgcag gcacagagta acgggctatt
    57961 cagcgaagcc gatctcaacc gcgagctaaa aaaacaacaa cgtattaccc cgcacatcat
    58021 cagccagatt atggagtttg ctgcaacgcg caaaggggtg atgattttcg ccgccaccgt
    58081 tgaacacgca aaagagattg tgggattact acccgccgaa gatgctgcac tgattactgg
    58141 cgacaccccc ggcgctgagc gcgatgtgtt aattgaagat tttaaagccc agcgttttcg
    58201 ctatctggtc aacgtcgcgg tactgaccac cggatttgac gccccgcacg tcgatcttat
    58261 cgccattctg cgccctaccg aatcagtgag tctttaccaa caaattgtcg ggcgcggtct
    58321 gcgtctcgct cctggcaaga ctgattgctt aattcttgat tatgcgggta atcctcacga
    58381 tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt gacaacgttc cggtacaggt
    58441 tttctgccct gcctgcggtt ttgccaacac cttttggggg aaaacgaccg ccgacgggac
    58501 attgattgaa cactttggtc gccgctgtca gggatggttt gaagatgacg acggtcatcg
    58561 cgaacagtgt gacttccgtt tccgttttaa aaattgcccg caatgtaacg cagaaaatga
    58621 tattgccgct cgccgctgcc gcgaatgtga cacggtactg gttgatccgg atgatatgtt
    58681 aaaagcggcg ctacgactga aagatgcgct ggtattacgc tgtagcggca tgtctttgca
    58741 gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc tattacgatg aagacggcgc
    58801 ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag cgaactgcct tcgagcagct
    58861 ttttatccgc ccgcatacgc gcacaccggg catccctctg cgctggatca ccgccgccga
    58921 tatcctcgcc cagcaagcct tattgcgaca cccggatttt gtcgttgccc gcatgaaagg
    58981 tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa ggtcgttttc gtcgggcgca
    59041 cgaattacgc ggttaatggt agttttgatt gatgtataag gcgattgagt atagaatctc
    59101 gcccgctttt gcatacgcaa agcagatcac ttacctgttg ctgggtcgcc tgtagcagga
    59161 ttaatttaag agagaaagaa atgtttacta tcaacgcaga agtacgtaaa gagcagggta
    59221 agggtgcgag ccgccgcctg cgtgccgcta acaagttccc ggcaatcatc tacggtggca
    59281 aagaagcacc gctggctatc gagctggatc acgacaaagt catgaacatt caagctaaag
    59341 ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg taaagaaatc aaagttaaag
    59401 ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca gcacatcgac ttcgttcgcg
    59461 cttaattgct gaataagttg taaaaaaccc cgctccggcg gggttttttg tatctgcaga
    59521 ttatgcctga ttacgatatt gctatttttt gcaggccaga taaggcgttc acgccgcatc
    59581 tggcattcat aacacaaaac cgattaattg ccgccggacg tccggcgctg caattgatcg
    59641 cgcaaattcg gcggcgtgcc tttaatagtc agggtatcgg tcgccggatc ccagaaaatc
    59701 cgttcgccca acagcatcgc atcaaaatta atagtcaagc caccgccgct accggcaaac
    59761 ttcgtcaact gacgcagtgt gctacggtct gccgggaagc tctcttccag ttcgtagcct
    59821 ttttcagccg caaactccgt gaagctcact tcgctgacgc ctgccagctc tttcgacagc
    59881 gattccagtt cgatctcttc gcctgcctgc agctgctcat tacagtagct ataaacctgc
    59941 tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt cggcagtgaa atcatcaacc
    60001 gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc cttcgctggc cccgaggaaa
    60061 tccataaaga aatccgccac tttgcgccct actcgccctt tcaggaaagt gagatagcgg
    60121 gtcgactctg gattggtttc ccattcggtt aaatcgatac gcgcaacaat atccgcatga
    60181 ttgatatcaa gataatgggt tgggttgatg tcgagatttt cgttaacgcg catactgctc
    60241 aggttgctca ggactgccac cagcagatac tccaccgcca gataacggta gtggcaaaac
    60301 agcacgaatc caccgtcagc aaaaggatat ttcgctaatt cgtcacgcag gcgaccggtt
    60361 gccgcgcggc taaatgccag aaagtcctct tcaccctgac gctgtaagcg cagcgtctgc
    60421 gccagttcac tctcttcgct aaacaagccg taagctttat ttttggcgct atagacccga
    60481 tgcagttctg ctaccatctc aacgacggtt tctgtcggtt ccagcaatga atcgcgcaac
    60541 accagctcaa ggttctgctc atcacgcttg ataagctggt gcagagcaat ctggttgata
    60601 tccagactca tgataaactc tcctttaaga ccgggcggta ttcaaccacc gccggttaag
    60661 cgacgcaata aaagatggcg gtcattttac agaaggcggc gtaacgagaa tgatttaacg
    60721 cataaaaaag cagaaaaaaa accgttgcta cggtaatatg ttgccctttc atcaactaac
    60781 tgatttcgat ttatgccaca aatttcccgc tacagtgatg aacaggttga acaattgctc
    60841 gcagagctgc tcaacgtact ggaaaaacat aaggctccga ccgacctttc cctgatggtg
    60901 ttaggcaaca tggtcactaa ccttatcaac accagcattg ccccggccca acgccaggca
    60961 attgccaact cttttgcccg cgccttacag tcctctatca acgaagacaa agcgcactaa
    61021 gggaaacaga taacaggtta tggtaactca tcgtcagcgc taccgtgaaa aagtctccca
    61081 gatggtcagt tgggggcact ggtttgcact gttcaatatt ctgctttcgc tcgtcattgg
    61141 cagccgttac ctgtttatcg ccgactggcc gaccacgctt gctggtcgca tttattccta
    61201 cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc acctacttgc tgattctctt
    61261 cccgctgacc tttatcgtcg gctcccagag gctgatgagg tttttgtccg tcattctggc
    61321 aacggcggga atgacgctat tactgatcga tagcgaagtc tttactcgtt tccatctcca
    61381 tcttaatccc atcgtctggc aactggttat taacccagac gaaaatgaga tggcgcgcga
    61441 ctggcagctg atgttcatca gcgtgccggt tattttattg cttgaactgg tgtttgcgac
    61501 gtggagctgg caaaagctgc gcagcctgac gcgtcgtcgg cgcttcgcgc gtccgctggc
    61561 cgcattctta tttatcgcct ttatcgcctc gcatgtggtg tatatctggg ccgatgccaa
    61621 cttctatcgc cctatcacca tgcagcgcgc taacctgccg ctttcgtacc cgatgacggc
    61681 gcgacgtttt cttgagaagc atggcctgct tgatgcgcag gagtatcaac gccgtcttat
    61741 tgagcaaggt aatccagacg ccgtttccgt tcagtatccg ttaagcgaac tgcgctatcg
    61801 cgatatgggc accgggcaga atgtcttgtt gattactgtc gatggcctga actactcacg
    61861 cttcgagaag cagatgcctg cgctggcagg ttttgctgag caaaatattt cgttcacgcg
    61921 ccatatgagc tccggcaaca ctacagacaa cggcatcttt ggcctgttct atggcatctc
    61981 gccgagctat atggacggca ttctgtcgac ccgtacgcct gcggcattaa ttactgcgct
    62041 taatcagcaa ggctatcagc tggggttatt ctcatcagat ggctttacca gcccgctgta
    62101 tcgccaggca ttgttgtcag atttctcgat gccgagcgta cgcacccaat ccgacgagca
    62161 gaccgccacg cagtggatca actggctggg acgctacgca caagaagata accgctggtt
    62221 ctcgtgggtc tctttcaatg gtactaacat tgacgacagc aatcagcagg catttgcacg
    62281 gaaatatagc cgggcggcag gcaatgtcga tgaccagatc aaccgcgtgc tcaatgcact
    62341 gcgtgattct ggcaaactgg acaatacggt ggtgattatc actgccggtc ggggtattcc
    62401 actgagcgaa gaggaagaaa cctttgactg gtcccacggt catctgcagg tgccattagt
    62461 gattcactgg ccaggcacgc cggcgcagcg tattaatgcg ctgactgatc ataccgatct
    62521 gatgacgacg ctgatgcaac gcctgctaca tgtcagcaca cctgccagcg aatattcgca
    62581 aggtcaggat ttgttcaacc ctcaacgccg tcattactgg gttaccgcag cggataacga
    62641 tacgctggca attaccaccc cgaaaaagac gctggtgctg aacaataacg gtaaataccg
    62701 cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa ccacagttaa gtttgttatt
    62761 gcaagtactg acagacgaga agcgttttat cgctaactga ttaattataa atcagttagc
    62821 gaaatatctt acttgcaatc ggtgtggaaa acggtagtat taacaaccac gagtcggcac
    62881 gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg gtcggaggtt caaatcctct
    62941 cgtgccgacc aaaaatccca aaaaaaccaa ccgtaaaggt tggttttttt atatctgcat
    63001 ttaattcgat aaacagaccg cgacacatca gggcctgttt attgtctgtt atcagaacgt
    63061 ccagaccaca cccgcctggg tattccacgg cgattctacc cctgcgccat tgccatagct
    63121 gactgacaaa tgaccgctta acgtcggggt aaacgatgac cttatcccag cctgataaac
    63181 gccacgggtt ccagccacat cattattaaa tttgccatcg tcattaactt tcacctggtt
    63241 agaatcggcg tattcctgac gcacggcggc tttcagccag ggttccagcg tcgtaccgtt
    63301 ttgcaggtcc atgtgatagc ttaccgccgt tcccgcttca gcgcgtaata tccgggtatt
    63361 tcccacatcc gcgcgcatgc cgtttgataa cgtgtagtcc tgaccatctg tggtaaagcc
    63421 ggtaaaggcc agatagggtc taacactcca caatccgtca acccaacgga acccgctctc
    63481 gacatgagca cccgcgccgt tactattgta atcgccaaac gctgttgccc cattactcat
    63541 cttgccatgg atggtgttgg caaaacggtc aactttcacc actccatcaa cataggcacc
    63601 gttctgatgc tcccaaccgg cataagcccc cagggtatag ctatcgacat tgcctttgcc
    63661 gccgcgatca aaaccaatat cagaatgaga gtaaccaaag aacaagccgc gaattgtgct
    63721 gctttcttca cgggagaaac ggctatcgat accgagcgtc aggcccgtca atgtttgctc
    63781 aaaaccagct cccgcatcag tggtcacgtt gttgcgggtg ttaattgccg aactccacat
    63841 cgccgtatcg taactaacgc cttttacgct accaagacgc tcacgcacgg tgtccagttc
    63901 tgcatcaaat accagcggtt gtgcggccgc catattcagc acatcagtgg ttgaaggggt
    63961 aatttgcgcg cgattctctg ccagactcca gctatggtta ccattatcca gcaaggtata
    64021 ttcatacgta ccgatatcaa caacgcctcc ggcattgccc aacgtaaatg cagcatcacc
    64081 gccgcccgtt gttaccagtg taaggctatc tcctgctgcc gggctggcac cggtgtccgt
    64141 cacgaatatt ttgaaatcac ctgttgcctg accggtgacg ttgagctgat cgctctgatg
    64201 attcgccata tcggtacgca tataaaaatt gccgtttccc gacagggtat tggtggtcaa
    64261 cgtagtgaaa ttttccgcac ttgccgtcac agatgaacga gtcactgact catccatcat
    64321 aatcacattt gcgccagaca tcgcaaggtt ggctaagtta aggcgggtgt tttggttcgt
    64381 cgtagtgata tcggcaatga tggtccatgc gctattttcg agcgaaacat ccgctttgct
    64441 ggtatccacg gcaccatgcg ttcttaaaga agcgccctca gaaaccacta ccttacccac
    64501 caacattgcg ggtgcataat ttatttgcgg cttcataacc tcaagaatgc catcatttcc
    64561 tctgactgac acgttaacca ttgtgccagc ccagacgtta gcgcggccat tgttgataac
    64621 catattttca gagatggctt tagaggaata cgttatactt ccattctgat ctgatcgacc
    64681 gagatcatat accgcatttg cctgcactct cgtaccagta tcctttccca gcgattgcat
    64741 agtggcatgc tcatcgagga tagtgtcaat ggcctgcgtg tcctccataa caataagccc
    64801 ggaaccatca tccagttcat aattttttga cacaccatct tttatactaa attggccttt
    64861 actgtttgta ccactcactt ccagcgcatt cgttgagaca attaattttc cgcctgcttt
    64921 tttatcaacg ccagttgcag tcccgccatc cataacctcc agtaagccgc cactatctac
    64981 agtagtatta taagcgaagt cattttcttc aactcgcaaa cttccgccgt tttccaacag
    65041 catattgtta gcaataccat tcttgatttc gaactgaccg agacggtttg ttccgaatac
    65101 ctccatgacc cgcgtgcttg ctttaatagc accgcctgct ttctgatcta ccgcaatcgc
    65161 taatcccccc tctttaacct ccagtaatcc tcccttttcg atagcggcaa ggacggtaat
    65221 cccatctttt tcaacgatta agcgcccgcc tttaatcgtg ggatcattcg ctgcgccata
    65281 aacctgtagc acaccattat tgagcgtagt ctggtttgcc gcgccaccca tttgaacaat
    65341 ttgataacca tcatttaata ctgagtattc tgcggtgcca ttcatttcaa tgtactgata
    65401 cccgccgtta atttcagtat tactacttat accaaattct ttaatatgct gttctccgcc
    65461 aggattgatt ttcgtattat tggcggtccc ataaaccagc tggatatctt tttccaggac
    65521 aacaccatca actgtctcac catactcaac tgttgatgcc aatgctatgg gtgaatataa
    65581 cgctgtagct accagaacag atattgcgga acgacaaaga gaaacagaac cagattgatg
    65641 cattgagctt tcatcctatg aaattaattg cagttaaaag cattgggtac agaaaatacc
    65701 catagctcca tacccggagt cagtttttaa aaactgttta aagaaatgca caagtattgt
    65761 gattgatttt ttagttgttt ttcttgatga gaagctgatg caaaattccg tctttataat
    65821 gaaaatgatg ccaaagcgaa cgacaaggtt gtagttttcg ctacatgtcc atacataaaa
    65881 tggggtaaca ttcacgcgcc tggtagcgtt accaacgcta cgctcaaaca taatgattct
    65941 aataaaacct caggagacta ctatgcctga agcaacgcct tttcaggtga tgattgtgga
    66001 tgatcatcca cttatgcgac gcggtgttcg tcagttactg gagcttgatc ctggctttga
    66061 agtggtcgcc gaagcgggcg acggcgcgag tgctatcgat ctggcgaata gactggatat
    66121 cgacgtgatc ttgctggatc tcaatatgaa aggtatgagt ggcctggata ccctcaatgc
    66181 cttgcgcagg gatggcgtta ccgcgcaaat tattatcctg accgtatcag atgcctccag
    66241 cgatgtcttt gcactgatag acgcaggcgc agacggttat ctgttgaaag acagcgaccc
    66301 ggaagtgttg ctggaagcga ttcgtgccgg agcgaaaggc agcaaagtct ttagcgaacg
    66361 cgtcaatcag tacttacatg aacgtgaaat gtttggcgcg gaagaagatc ccttcagcgt
    66421 gctgacggag cgcgagctgg atgttctgca cgagctggca caggggctgt caaataaaca
    66481 gattgcctcg gtgttgaata tttccgagca gacagtaaaa gtacatattc gcaatctact
    66541 gcgcaaactc aatgtccgct cacgcgtggc agccaccatt ctgttcctgc aacaacgcgg
    66601 ggcacaataa aaatagcccg atggcatcac catcgggcct ctttttattt actctcctgc
    66661 ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa tcaccgcacg gcgagtatcg
    66721 ttggcaggta agagtttcaa catcatctcc cacgcggcaa cggcttcgcc aaatcgctgc
    66781 tgctcaaagg cattaaacgc atacatgctt aacacacgaa tattgctatg gtccgttctc
    66841 accaactggc gcagcagttc gccgccgagg cggttgtcgt tgggatcaga tgaacgtgtc
    66901 aacgcttcag cataacccag tgtggcatcg ctgtttttcg gatcgaggcg atacgcagta
    66961 gcgtatgcat cggtggcgat actggcgtta cccagcgcca tgccaacgcg acccaacata
    67021 atccaacctt ctatatctcc cggatttttt tgcagttgag tacgcatccc cagcgcaagg
    67081 cgcgacatct cttcttcgtt gagcggatcg gctttcggat ccagcgccct gtccagcaac
    67141 gccggagcct gtgccgtggc ctgctgccag attttcacct gctgataatt gccagtctgg
    67201 tagtagctga cgccagccac aattaacgcc accacaatac ccggcagata aacaacatat
    67261 ccggcacgct taccttccgg cacgctttgt tcaggaaacg cttccggtac cacgcgtacc
    67321 cgacgccgcg aacgggcgta tatgacccaa ccgccaatac caatagccac taccggaagc
    67381 acccacagca gcacggtcag cggcgttaac ggcggatcgt aagtgacgaa gttgccgtaa
    67441 cgcgccacca tataatcgac aatctctttc ttacttttgc cttcctgcat cagttcatac
    67501 actttctggc gcaggtcggt ggcaatcatc gagttggaat cggcaatgct gttattctgg
    67561 catttcgggc agcgcagttc ttcagtgagc tgacggaact gttgttcctg tgcttcatct
    67621 ttaaactgca acacgtcgat ggtcgccagc gctgagccgg agatcatcag catcagcacg
    67681 cccaataaaa acctcattgt gcggcctcct tactgtattt ctcccacagc ggcttgatct
    67741 cttcttccca gacgcgagga ttgagatcgc ccgcatggcg atagcgaatg atgccgttgc
    67801 cgtcaataag aaacgtttct ggcgcgccat agacaccgag atccagccct aacatgccat
    67861 cgccatcaaa aaggcttagc gcgtaaggat tgcccagctc tttcagccag ctgattgcct
    67921 tctggcgatc gtctttatag ttcatgccga ccacgcggat gccctgcgca gaaagctgat
    67981 tcagatattg atgttccgca cggcaggtcg gacaccaggt cgcccagacg ttaagcagca
    68041 ctggtttgcc ctgagtcagc acatccgcct gataaaactg ccccggattg tccagtgatt
    68101 cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga ttccagattg gtcggatcat
    68161 ccccttcggc attacgcgcc agctgccaca gcagcgccgc ggcaatcgcc aggaagataa
    68221 tcaacggaat taacaatact ttgcgcttca tacagcctcc ggcgcagttt tttgcgggct
    68281 cacgcgctta cgatagcgag gatcaaacag acacagcagt ccgcccaacg ccatcatcag
    68341 cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa agacgcacag cccacgcgcc
    68401 gttttccagc tcttcaccga gcgcggcgta caggtcacgc gtgatgccgc cgtcaattgc
    68461 cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt ttttccgcat acagcaccgt
    68521 ttccggttta ccctctcgcg ttacgccgat agtcgccaca ccgccacgcc agttcgggcc
    68581 agtcacctct ttgacatcac ggaaggtgaa gcgatattca tgaatatcga cgctatcgcc
    68641 ggacttcatg cgcacatcac gctcaacgct atagttctgg ctaaaggcaa tgccaacaat
    68701 tgtcactgcc agccccaggt gagccgccac catcccccaa taactgaagg tggttttcgt
    68761 gccgcgtgaa atacgtagcg cagcttccgc aattgccagc accgcaatcc agcaggccat
    68821 tgccaggccg agcaccgtca tcgccacaac tttgctttcg aacagccacg gcaacagcag
    68881 agacagcacc agcgtagaga tgaaggcgat aatcaataaa ttgcggattt tacgtgggcg
    68941 atcgcgcccc cagcgcacca gaggaccgac acccagcagt agcgcaaacg gcaccatcag
    69001 ccaggtaaac atggtgttga agaacggttc gccaatcgaa atactgccca gtcccagttg
    69061 cttgtgcacc agcggcagca gcgtccccag caacaccacc agcatcgcgg cgaccagcaa
    69121 aacgttgttc gctaacagca aagattcccg cgaccacagc gcattgttta cgcgtgagcg
    69181 aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg ccaatcacca gcaccataaa
    69241 ggcgaggata aacataccgc gcgccggatc agacgcaaac gcgtgtaccg ataccagcac
    69301 gccggaacgc acgaggaaag tccccaacag acacaacgag aaggcactga ttgccagcag
    69361 taatgtccac gctttgaagc tggcgcgttg ttcagtgacc gccagtgagt gcatcagcgc
    69421 agtccccacc agccacggca taaacgaggc gttttctacc ggatcccaga accaccagcc
    69481 accccagccg agttcgtaat aggcccatgc ggaaccgagc acgatgccga gcgtcaggaa
    69541 gatccacgct gccagcgtcc acggacgagt aaaacgcgca taagtgctgt ccagacgccc
    69601 gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag aaacccacgt accccatata
    69661 aagcagaggc ggatggaaga tcagccccgg atcctgtaac agcgggttaa gatcgcgccc
    69721 ttcaatcggg aagttcggca gcgtgcgtga gaatgggtta gaggtaaaga gaatgaacag
    69781 caaaaagccg acactgacca tccccattat cgccagtacg cgggccacaa tatccagcgg
    69841 aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg ctcatcagca gcacccacag
    69901 cagcagtgag ccttcatgtg cgccccaggt agccgccacg cgataccaca ccggaagctg
    69961 ggtattggag ttgctggcaa cataggtgac ggtaaagtcg ttgaccacga aagcattgac
    70021 cagcaccaga aatgcgccag ccacagacat aaacagcagc caggcaaaca agcgggaaga
    70081 cgccatcatg cgcgcatctc cgcgcgccac gccccatagc ggatacacgg acagcagcag
    70141 cgcaattccc agcgccaggc acagcagccc gttaccaatt tccggcatca tgatgctggg
    70201 tccttataaa cactcgccgg gcgacggtgg ttagcttcca tcgctttctc aacttctggc
    70261 ggcgtgtagt tttcatcatg tttcgccagc acttcttttg cgaggatatg attgcctttt
    70321 tccagctcgc cctgcaccac aacgccctgc ccttcacgga acagatccgg caaaatgcct
    70381 tcgtaagaga catccactga gccttcagcg tcgtaaatgg tgaaggtcac tttcagcgaa
    70441 ttgggatcgc gctgcacact acccggcatc accatcccgc caacgcgcag acgctgaccg
    70501 acttccggca tctgctgagt ttcacgcttg ccgtagagaa tttcccccgg cgtataaaag
    70561 agatcgatat tcgagcgcag cgcatatagc accagaccga tagtcagcgc cagccctgcc
    70621 aacacggcac aggcaatcca caagcggttt ttacggcgaa tattcatgca gcctcctgct
    70681 gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg cgccacacca cgcagaattg
    70741 cgcgatgctg catcaccgag tgcacgacca aaaccaccag cggaataacg gtcatcacca
    70801 ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa aaattcattc caggaagcaa
    70861 atgcaggggt catttacggc ctcttttcag tatcagttca ctcacccacg gacggcgttt
    70921 ttccatcagc aaaatcaaat tacgcatccg catcagcgtc agcgtggcag agaggagcag
    70981 gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc ggatcgatac tttgctgcat
    71041 ccgcgttgat ccctgatgca gggtgttcca ccactccacg gagtaatgaa taatcggcag
    71101 attcaccacg ccaatcagca ccaggatacc tgccgcacgg cccgccagac ggcggtcatc
    71161 gaaggcgtgc cacagggcaa tcacacccac atacaaaaac agcagcacca gttcagaagt
    71221 cagacgtgca tcccataccc accaggtgcc ccacatcggt tttccccatg cagagccggt
    71281 aaccagggca ataaaggtaa acacggcacc aatgggggcc atcgccgcca ccgccaggtt
    71341 ggccattttc atctgccaga caaggccaat aaacgccgcc accgccattg atgcataaat
    71401 gcccatcgac cagatcgccg caggcacatg caggtagata atgcggtagc tatttccctg
    71461 ctgataatca gccggagcaa agccgaatcc ccagatccag ccgacggtaa gcacgactac
    71521 actggcaatt gccagccacg gtataaacca gccacagatt tgatacagcc gtggtgggat
    71581 cgccagttga tgcagtgttt tccacatagt ttcgatacca gactcgaaca aaaatcagta
    71641 atccagcgtt attgaatgct gattcgtaac gctgccgccg tcgcaaaagg acttaatgtc
    71701 gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc cgtcaacggg caaatgcata
    71761 gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg ggatagtcag cggtaacacc
    71821 agtatgctga gcagcacacc accgcgctta agtcccactg tcagcgccac gcccggtgca
    71881 ccgagaaagc caagcgtagg cgttcccagc agtagcgtta acgccatcac ttgccagcca
    71941 taaacatcca ttcccagtag cattgctacc agtggcgaaa ggatgagtaa cggcagaccg
    72001 gttaccatcc agtgcgccat caccttcgcc agcacaacgg cgggcaaggg taacggcaac
    72061 aacatcaatt gttcaagact gccgtcctgc aaatcgtcac ggaacagtcg ttccagcgcc
    72121 agcaaggatg aaagcagcgc ggcaacccag ataatgcccg gtgcaatacg tgccagcagt
    72181 tgcggctccg gaccgatact gagcggaaaa agggtaatta caatcaggaa gaaccacagc
    72241 gggttggcga tttcagcgct atggcgaaac gctacgcgca gctcaagacg gaaaatgcgc
    72301 cagaacatca tacggccctc gtttgcgtca gtgaaatgcg gcgaatttta ctttcagcaa
    72361 cgttgagcgg ctggtgggta gtgagaatca caatcccccc ctgctctgta tgctgcgcca
    72421 tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc ggtaaaaggc tcgtcgagga
    72481 tccataacgt ggcacgggtc agccacagac gcgctaaagc gacgcggcgt tgttgcccgg
    72541 cagaaagttg atttaccgga atatcttcga atccggcaag cccggcctgc gccagggctt
    72601 ccagacattg tgcggtatcg ccatcgcgat gataaaagtg cagattttct aacgccgtca
    72661 gccgggtttt gatccccggc tgatggccta tccataacag gttttgatgg tagctgtcgc
    72721 gtacctgatg caagggctgc ccttgccaga gaacctcgcc tgcgtcaggg cgagacaacc
    72781 ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt gctaccggtg atttgtaccc
    72841 actctcctgc gttcagcgta aatgacaagc cactaaataa ggttcgttca tcccgctcac
    72901 aaagtaactc tctgacttca agcataccca cgcaataacc ctgttaaaaa cctggctcga
    72961 cttcacgcat atcgggcagc ttgtgcgcta tccctttatg gcaatcaata caggtttgcc
    73021 catctttcac cgcctggtca tgcatcttcg cggcaaccga tttctgggcg gttgtatcca
    73081 tatactcgaa gttgtgacag ttacggcact cctgcgagtt attgtccttc atgcgccgcc
    73141 actcattctg tgccatcgtc agacgatgag cttcaaattt ctgcggcgtg tcaataacgc
    73201 caaaaatttt accatacagc tctttacttg ctttgagctt gcgtatcatc ttcggcacaa
    73261 actcgtgcgg aacgtgacaa tccggacagg tcgcacggac gccgctacgg ttgttgtagt
    73321 gcacggaatc catgtattcc tgatacaccg tgttacgcat ttcgtggcag ctaatgcaga
    73381 actcttcggt attggctttt tccatcccag tgttaaagcc gccccagaag acgatgccgc
    73441 cgacaaaacc gatcaacagc agcgtcccca gcgccagacg gctgggggta cgccaccatt
    73501 tccacaggcg cttaatcaga ccaggcttac ggtcagaatt tcccataata acctcttatt
    73561 tcccgtaacc ttttgatggg gtaaaggtat tccccacgat tggcgcggta tcggcctgcg
    73621 gtacgtgaca ttgcagacag aaataacgac gcggagccac ttccgcgccc actttgccgt
    73681 cgctgtccat aaagtgagta ggactgatac gcggcgcgcc agtggtgcga tagctttcga
    73741 caccgtggca ctgcaagcag cggttggtat tggtcgttac ctgataacct tcaacgctat
    73801 gcgggatcat tggcggctga ttcacatagt tcagcggcat ccgatcctgc tcttttggca
    73861 tccgaatggc cccttcctgc gtcccggata cttccggcga ctggctaaaa tcgacgccat
    73921 tagccgccca aaccgcgccg cttaccacca gggccagcat cgccgtccat tgacacagcg
    73981 ctttcttcag gtcatggctt ttcatgattt cgctcccgaa ctccatcgtg tagttattgt
    74041 aaatacatcc tcagaacaga catccacgca gcgaccgcac gtcatgcaat cgcggctggt
    74101 gacctgcacc gggctttgtt catccagcac cggggcacgt agcacatgcg gttccgggca
    74161 aacatgaaaa caatccatac agcggttaca tttctgccga tcgctggccg caacggtaat
    74221 cacacctttg ctacccagca cgccatacag cgcgccaacc ggacaaatgt gcccgcacca
    74281 gccgtgttca acgaccagta gatcaaataa aaacagcgcg agaataagca gcgcgccgct
    74341 gccgaagccc attaccaggc tacgccccat cagggaaacg gggttgatcc attcccagat
    74401 gagcgtgcca gttaaggctg atcccaccag aatgaccacc aacagcacgt agcgaatgtg
    74461 gcgagggatc gtcgcagact gattgaggtc aaagcgcctg cgtaaccagt tcgctaagtc
    74521 ggtaatcggg ttcagcgggc agacccagct gcaaaataat cgctttcctg ccagggcgta
    74581 gagcacggtg ataatcaccg caccggtcaa cgccaccgtg gcaggcagat gaccactggc
    74641 aagactttgc agcgtcatca gcggatcggt cagcggaacg gtgtcgaata acaggctact
    74701 gctatagttg ccgtgcaaga tccacacacc aaaccacgga ccgctcaaaa acatccccag
    74761 cacgaagaac tgacaaaggc gacgtaacac cagccaacgg tgactgcgcc accagccttt
    74821 tttctccagc gcctcgcgcc cggcgtcacg tttacgattt gccattgttc ccctccagcc
    74881 agccgaagcg gtaatggtga cctaactccc ctttcgccag tgacagcggt aacaccttga
    74941 ttgccggttg ttccagcacg catacttttt cgcacttacc gcaaccggta caggcgtcgc
    75001 tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg cgtgttgcgc tccagctcca
    75061 gggtgatggc ctcatcaatt ttcgggcatt cgcgataaca aacatcgcag cgcagcccct
    75121 gaaagttgag acagttttcc tggtccacca gtaccaccag ccccatccgc gcgtcgtcga
    75181 tcgattcaat ctcacgatcc agcgcaccgc tcgggcacac tttggcgcac ggaatgtcct
    75241 cacacatttc gcaaggaata tcccgtgcga caaaatatgg cgtgcccgcc gacaggccag
    75301 aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc ctgaacacac tgaccgcaac
    75361 gcacacaggc actggcaaag gcgttctcgt ttatggctcc gggcgggcgc aaccgcacgc
    75421 cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac acccacggca gccagcccgc
    75481 ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc attttgaggt ttcgctgacc
    75541 gggacataat ggcttacacc ttctccagtt tgaccgcgca cttcttgaag tccgtctctt
    75601 tcgagagcgg atcggtcgcg tccagcgtta gtttgttaac cagctgtgcg gcgtcgaaga
    75661 acggcatgta caccaggccc tgcggcggac ggttacgacc gcgcgtttcg acaatcgaga
    75721 tcacttcgcc acggcgagaa accactttca ctttgtcgcc acggcgcaga tcgcgcgctt
    75781 tcgcatccag cgggtgaata aacaggaccg cttccgggaa ggcacggtgc agttccggca
    75841 cacggcgagt catactgccg gtgtgccagt gctccaggac gcgtccggta gagagccaca
    75901 ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa cggcagcgcg aagatcaccg
    75961 ctttaccatc cggtttaccg tagaacttat agccttcgcc cgctttcacg tacgggtcgt
    76021 taccttcgct gtaacgccac tgcgtttctt taccgttcac caccggccag cgcagaccgc
    76081 gcgctttgtg gtagtcatcg aacggcgcga gatcgtgacc gtgaccgcga ccaaaccatg
    76141 cgtactcttc gaacagccct ttttgcagat agaagcccag ctcgcgggat tcatcgttca
    76201 gctgatcttc cgccagttcg gataccggga atttgctcac ttcgggggtg gcatacagaa
    76261 cttcgtacag cgttttgcca cgcagttccg gtttcttcgc cagcagctct tccggccata
    76321 cttcttcagt tttgaagcgg cgggagaact ggactaactg ccagaggtcc gatttcgctt
    76381 cgcctggcgc ctgtacctgc tgacgccaga actgagtacg gcgttcggcg ttaccgtaag
    76441 ccccctcttt ttctacccac attgcggtcg gcaggatcaa gtcggcggcc agcgcactga
    76501 ctgtcggata cggatcggag acgatgatga agttgcgcgg atcgcgccag cccggcatac
    76561 gctcttcatt aatgttcggc ccggcctgca tgttgttggt acacatggtc cagtaaacat
    76621 tcagcttgcc gtctttcagc gcacggtctt gtgctaccgc atgcagaccg attttcgccg
    76681 gaatggtgcc gctcgggata ttccacttct tctcgcagat atcacgatgt ttctcgttag
    76741 ttaccaccat gtccgcaggc agacggtgag caaaggtgcc cacttcacgc gcagtaccac
    76801 acgcggaagg ctgcccggtc agggagaacg gaccgcaacc cggctgggaa attttgccgg
    76861 tcagcaggtg caggttgtag accaggttgt tggcccacac gccacgagta tgctggttga
    76921 agcccatcgt ccagtaggag atgactttct tgttcggatc ggcatacagc tgcgccagtt
    76981 gttccagctg atcttttggt acgccagtca tttcagcggt tttttccagc gtatactcag
    77041 caacgaaggc tttgtaatct tcaaagctca tcggttcgga ggcgtcagaa cccggattct
    77101 tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta gccgatgtcc gtcgcccctt
    77161 tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat cgcattgttt tgaatgatat
    77221 agttggcgat gtagttcagg atcaccaggt cagattgcgg cgtaaagatg atgccgttat
    77281 ccgccagctc gaagctacga tgctggtagg ttgaaagcac cgccacggtg acgtcctggt
    77341 tagagagacg acggttagtg atgcgtgacc agaggatcgg gtgcatctcc gccatgtttg
    77401 cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc atagcagccc atcggctcat
    77461 ccataccaaa ggttcgcata aagccaacta ctgccgacgc catacagtga cgcgcattcg
    77521 ggtcgatgtt gttcgaacgg aagcccgctt taaacagctt ggacgcggca taaccttccc
    77581 agatagtcca ctgaccagaa ccgaacatac cgatcgattc cggccctttt tctttcaggg
    77641 cggttttgaa tttctcttcc atcacatcga aggcctgatc ccaggtgatt ggggtaaatt
    77701 cgccttcttt gtcatattta ccgtttttca tacgcagcag cggctgcgtc aaacggtctt
    77761 taccgtacat gatcttgggc aggaaatagc ccttaatgca gttcaggcca cggttaaccg
    77821 gtgcgtccgg gtcgccctga caggccacca cacgcccctg ctgcgttccg accagaacgc
    77881 cgcaaccagt accgcagaaa cggcacggcg ctttatccca tttaatggct tcctgctgac
    77941 caacaacggc gcgggcaacg cccggcacgc tgagaccggc agccgccgca gcggccgcaa
    78001 cggcgttagc tttcataaag ctacgacgac tgagtttcat ggtgtttcct caccttgctc
    78061 ttcctgctgg tgataaacca gcgacaccgc cagcacgccc tctacgttgc gtactgactc
    78121 aatggtttgg atcagcgttt cgctgtcttc tgcttccacc accacaatca actgaccgct
    78181 cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg ttcagttggg tgctgatgtc
    78241 tgaaattcgt tcgcttttgg cctgcacaac caggctgcaa acttgccagt tagtgtgcat
    78301 ggagatactc cgcagttatg gctgataccg gacagctggc tgcacatgct ccacatccgt
    78361 tgcaaagttg gctattaagt tgcggctggt agatcccgga gagcgtcggg cgaaagataa
    78421 ttgccattgg ctcacagcta tcctgacagc ggcggcattc aactgactga tacgccaggc
    78481 acgcgtcccc aatggtgaac tgcaaatccc aggccctggt gtggcgcgga gaaaatagcg
    78541 attcggggca agcctgcgcg caggcgtaac agaagctgca ctcgttattt ttgaaattaa
    78601 cgctcggata gccgcccgcg ccgcgttgca gaatgttgtt ttcacaagca ttaatacagg
    78661 cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc accggaccag ggcggacgga
    78721 taccgttact ggctttgcgc cagcgaccag tgagtatgcc ccgacgggat gcatcaatct
    78781 tcacattgac cttccatcat taacgcgctc tgaaaattga gagcgaccaa ataaaccgca
    78841 taattaataa gccattttta tagccgctaa gatattaaag gatgtgtcaa agatgcatac
    78901 cccgatcgag gtaaaacctg tagcaggatc aaaagagtgg cgggaagcgt ggcaaaaacg
    78961 ggcttttgct cacatttcaa atggttataa atatatttat atagcgatta attcaccaga
    79021 gatatttctg ctggtttgct ctctcatcag aatttaacac taaaagagca ggtaaaattg
    79081 tctgaatatt ctttaagtta ttcataaagc aaattaataa atctgatgaa tatgttaacc
    79141 ttcagcgaca tcatcggtgc aaacctataa atgaagaagg aaagcaaaaa atgaagacca
    79201 ttctacctgc agtattgttt gccgctttcg ccaccacttc cgcctgggcg gcagagagcg
    79261 tccagccact ggaaaaaatc gcgccttatc cacaagctga aaaaggcatg aagcgtcagg
    79321 tgattcagtt aaccccgcaa gaagatgaat ctaccctgaa agtagaactg ttaatcggtc
    79381 agacgctgga agtcgattgc aatttgcatc gtctcggcgg gaagctggaa agcaaaacgc
    79441 tggaaggctg gggctatgat tattatgtct ttgataaagt tagctcgccg gtttccacga
    79501 tgatggcctg cccggatggc aagaaagaga agaaatttgt caccgcgtat ctgggcgata
    79561 ctggaatgct gcgttacaac agcaagctgc cgatcgtggt gtatacgcca gacaatgtag
    79621 atgtgaagta ccgcgtctgg aaggcggaag agaaaattga caacgcggta gttcgctaaa
    79681 ctgccgtgaa gtgcggcacc ccgtaggttg gataaagcgg tcacgccgca tccgacatct
    79741 aacgcccgag ccggttgcct gatgcgacgc tggcgcgtct tatcagcata cgccacatcc
    79801 ggcataccat gccggatgtg gcacatcatt acaacgcaat atccgccact tctttttgaa
    79861 cgggttgcgg tttcaactgc ggtttcggcg tactatctgc tgcttgcggt ttgtcgtatt
    79921 tcagccccag cacttcgctg gtgtactgca actcgcgttc tgttgccgct acatcaccgt
    79981 tcagcttgcg tccataagat ggaacgatcg ctttcaacgt agcctgccat tgcgggctgg
    80041 aaacgcgatc gccaaatact ttttccagca gattcaacat aatcggcgcg gcggttgacg
    80101 cccctggcga tgcccccagg agcgcggcaa tggttccttg ctggtcactg accacttcag
    80161 tacccagacg cagtacacca cctttatcgg catcacgctt gataatctgc acacgctgcc
    80221 ccgcttgcca caaacgccag tcctcttttt tcgcttgcgg atagtactct ttcaacgctt
    80281 caaaacgatc ctcttcactc aacatcacct gactcaccag atatttcacc agatcgaaat
    80341 tatccagccc gacgtgcatc atcggcatca cgttagaggt ggtggtggaa ctcatcagat
    80401 cccacaatga accgtttttg aggaatttgg ttgagaaggt ggcaaatggc ccaaacagca
    80461 ctacgcgttt accgtccaga acgcgggtat cgatatgcgg aaccgacatc ggtggtgcgc
    80521 caacggatgc tttaccgtaa accttcgcca gatggtgatt aaccacgtcc gggttttccg
    80581 aaacaaggaa ctgtccgccc accgggaaac cggcgtagtc tttcgcttcc ggaatccccg
    80641 attcctgtaa cagcttcagt gccgcaccgc ccgcgccgat aaagacaaat ttcgcacgga
    80701 tgttctgtgc agtgccattt ttcagatcgg caacggtaac ggtccaggtg ttatcgtcat
    80761 tacgctttag ggcgcggact tcgctgctga gttgcagcga gaagttagat ttcttctgca
    80821 aggaagcaat taactggcgg gtgatctcgc cgtagttcac atcggtacca atttccgtac
    80881 gcgtggctgc cactttctgt tgcggatcgc gcccttccat cactaacggt gcccactctt
    80941 tgatctgcgc gtgatcttca gagtaacgca taccgcgaaa cagcgagctt tgttgcaacg
    81001 cggcgtaacg ggcgcgcagg aaattgacgt tatcctcgcc ccagacaaag ctcatatgcg
    81061 gaacggtatt gataaatgaa cgcggagtac gcagcacgcc gcgctcgacc tggtgcgccc
    81121 agaactggcg ggaaatctga aatgcttcgt taatggcgac tgctttttca atactgatgc
    81181 tgccatcggc gttttgcggg gtgtagttca gttccatcag tgcagaatgc ccggttccgg
    81241 cgttattcca gccgttcgaa ctctcctgcg cgacaccctc caggcgctcc accatggtca
    81301 tcgaccattc aggctccagc tcgcgtaaat aggtccccaa cgtggcgctc ataatgccgc
    81361 cgccaatcaa cagtacatca gtttcctgct cctcggacgc tttcgctttt gccgccatcg
    81421 aaacggcatt aagccccacg gccatcgaga agagcatggc agtcactttt ttcatcttgt
    81481 taatgcctta cttttagtcg ctttattgca ggtgagattt gcgcggcatc aacggtaaca
//
LOCUS       DAZFDC010000007        77181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFDC010000007 DAZFDC010000000
VERSION     DAZFDC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.007"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..886
                     /gene="espX2"
                     /locus_tag="ACHJ48_000554"
     CDS             <1..886
                     /gene="espX2"
                     /locus_tag="ACHJ48_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO4706179.1"
                     /translation="YLSLSEIKQFVTEEIKLNFNEQQGLLDKDNKCYILLSSDNSGRV
                     MRLSHQALISMLEPEVKKKTIWNNYSIYPSLQDTHEVVRDDPETICMRAFPLFAKGWE
                     YAQKNKKHQLILNALGFKGYIRDIFMSAIMRKTDFVLECNNQPTELNSSFSSLMNDSD
                     QWQQHTLKDKHYANLLTMLDLNDASESDKSKIFFCLSAVFANISHSNVFNGIPDASKT
                     LKRYAFALLAKAHSLDESMISNQTFNTYKTVLLDFNNLSNEEANQLRISSLYRDMVRY
                     AQYRFSKVLSEWTPDAWL"
     gene            1170..3320
                     /gene="dinG"
                     /locus_tag="ACHJ48_000555"
     CDS             1170..3320
                     /gene="dinG"
                     /locus_tag="ACHJ48_000555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO4706180.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDMLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3348..4310
                     /gene="ybiB"
                     /locus_tag="ACHJ48_000556"
     CDS             3348..4310
                     /gene="ybiB"
                     /locus_tag="ACHJ48_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO4706181.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREEMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIQRCLAGREPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4451..5536
                     /gene="hcxB"
                     /locus_tag="ACHJ48_000557"
     CDS             4451..5536
                     /gene="hcxB"
                     /locus_tag="ACHJ48_000557"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO4706182.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5603..5662
                     /gene="ybiE"
                     /locus_tag="ACHJ48_000558"
     CDS             5603..5662
                     /gene="ybiE"
                     /locus_tag="ACHJ48_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO4706183.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5765..6025)
                     /gene="ybiJ"
                     /locus_tag="ACHJ48_000559"
     CDS             complement(5765..6025)
                     /gene="ybiJ"
                     /locus_tag="ACHJ48_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO4706184.1"
                     /translation="MKTINTVVAAMALSALSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(6290..6556)
                     /gene="ybiI"
                     /locus_tag="ACHJ48_000560"
     CDS             complement(6290..6556)
                     /gene="ybiI"
                     /locus_tag="ACHJ48_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO4706185.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKESQLR"
     gene            complement(6632..7318)
                     /gene="ybiX"
                     /locus_tag="ACHJ48_000561"
     CDS             complement(6632..7318)
                     /gene="ybiX"
                     /locus_tag="ACHJ48_000561"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO4706186.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFIWIQSMIRDDKKRAMLFELDKNIQNIQSLKSRYGENEEILSLLNLY
                     HNLLREWSEI"
     gene            complement(7360..9642)
                     /gene="fiu"
                     /locus_tag="ACHJ48_000562"
     CDS             complement(7360..9642)
                     /gene="fiu"
                     /locus_tag="ACHJ48_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO4706187.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9907..10167)
                     /gene="mcbA"
                     /locus_tag="ACHJ48_000563"
     CDS             complement(9907..10167)
                     /gene="mcbA"
                     /locus_tag="ACHJ48_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO4706188.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10443..11369
                     /gene="rlmF"
                     /locus_tag="ACHJ48_000564"
     CDS             10443..11369
                     /gene="rlmF"
                     /locus_tag="ACHJ48_000564"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO4706189.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(11366..13591)
                     /gene="ybiO"
                     /locus_tag="ACHJ48_000565"
     CDS             complement(11366..13591)
                     /gene="ybiO"
                     /locus_tag="ACHJ48_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO4706190.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEF
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQILSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLGSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13708..14430)
                     /gene="glnQ"
                     /locus_tag="ACHJ48_000566"
     CDS             complement(13708..14430)
                     /gene="glnQ"
                     /locus_tag="ACHJ48_000566"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO4706191.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(14427..15086)
                     /gene="glnP"
                     /locus_tag="ACHJ48_000567"
     CDS             complement(14427..15086)
                     /gene="glnP"
                     /locus_tag="ACHJ48_000567"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO4706192.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(15224..15970)
                     /gene="glnH"
                     /locus_tag="ACHJ48_000568"
     CDS             complement(15224..15970)
                     /gene="glnH"
                     /locus_tag="ACHJ48_000568"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO4706193.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(16374..16877)
                     /gene="dps"
                     /locus_tag="ACHJ48_000569"
                     /gene_synonym="pexB"
     CDS             complement(16374..16877)
                     /gene="dps"
                     /locus_tag="ACHJ48_000569"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO4706194.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(17176..18063)
                     /gene="rhtA"
                     /locus_tag="ACHJ48_000570"
     CDS             complement(17176..18063)
                     /gene="rhtA"
                     /locus_tag="ACHJ48_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO4706195.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            18298..18363
                     /locus_tag="ACHJ48_000571"
     CDS             18298..18363
                     /locus_tag="ACHJ48_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706196.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            18416..18931
                     /gene="ompX"
                     /locus_tag="ACHJ48_000572"
     CDS             18416..18931
                     /gene="ompX"
                     /locus_tag="ACHJ48_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO4706197.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18980..20563)
                     /gene="opgE"
                     /locus_tag="ACHJ48_000573"
     CDS             complement(18980..20563)
                     /gene="opgE"
                     /locus_tag="ACHJ48_000573"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO4706198.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTKGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20835..20963)
                     /gene="mntS"
                     /locus_tag="ACHJ48_000574"
     CDS             complement(20835..20963)
                     /gene="mntS"
                     /locus_tag="ACHJ48_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO4706199.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21149..21616
                     /gene="mntR"
                     /locus_tag="ACHJ48_000575"
     CDS             21149..21616
                     /gene="mntR"
                     /locus_tag="ACHJ48_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO4706200.1"
                     /translation="MSRRAGTPTAKKVTQLVNEEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21613..22731
                     /gene="ybiR"
                     /locus_tag="ACHJ48_000576"
     CDS             21613..22731
                     /gene="ybiR"
                     /locus_tag="ACHJ48_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO4706201.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVTL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(22789..23709)
                     /gene="ldtB"
                     /locus_tag="ACHJ48_000577"
     CDS             complement(22789..23709)
                     /gene="ldtB"
                     /locus_tag="ACHJ48_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4706202.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23928..25520
                     /gene="ybiT"
                     /locus_tag="ACHJ48_000578"
     CDS             23928..25520
                     /gene="ybiT"
                     /locus_tag="ACHJ48_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO4706203.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(25688..26953)
                     /gene="ybiU"
                     /locus_tag="ACHJ48_000579"
     CDS             complement(25688..26953)
                     /gene="ybiU"
                     /locus_tag="ACHJ48_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO4706204.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPSQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(27105..27920)
                     /gene="ybiV"
                     /locus_tag="ACHJ48_000580"
     CDS             complement(27105..27920)
                     /gene="ybiV"
                     /locus_tag="ACHJ48_000580"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO4706205.1"
                     /translation="MSVKVIVTDMDGTFLNDVKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(28066..30497)
                     /gene="ybiW"
                     /locus_tag="ACHJ48_000581"
                     /pseudo
     CDS             complement(28066..30497)
                     /gene="ybiW"
                     /locus_tag="ACHJ48_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
     gene            complement(30503..31402)
                     /gene="ybiY"
                     /locus_tag="ACHJ48_000582"
     CDS             complement(30503..31402)
                     /gene="ybiY"
                     /locus_tag="ACHJ48_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO4706206.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQSLTVCGE
                     VKSVEEIMVTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            31533..32195
                     /gene="fsa"
                     /locus_tag="ACHJ48_000583"
     CDS             31533..32195
                     /gene="fsa"
                     /locus_tag="ACHJ48_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4706207.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTTEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPRA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(32374..33123)
                     /gene="moeB"
                     /locus_tag="ACHJ48_000584"
     CDS             complement(32374..33123)
                     /gene="moeB"
                     /locus_tag="ACHJ48_000584"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO4706208.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHNLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFSENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(33123..34358)
                     /gene="moeA"
                     /locus_tag="ACHJ48_000585"
     CDS             complement(33123..34358)
                     /gene="moeA"
                     /locus_tag="ACHJ48_000585"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO4706209.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLHRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            34562..35527
                     /gene="iaaA"
                     /locus_tag="ACHJ48_000586"
                     /pseudo
     CDS             34562..35527
                     /gene="iaaA"
                     /locus_tag="ACHJ48_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            35514..37385
                     /gene="gsiA"
                     /locus_tag="ACHJ48_000587"
     CDS             35514..37385
                     /gene="gsiA"
                     /locus_tag="ACHJ48_000587"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO4706210.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAVARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            37405..38943
                     /gene="gsiB"
                     /locus_tag="ACHJ48_000588"
     CDS             37405..38943
                     /gene="gsiB"
                     /locus_tag="ACHJ48_000588"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO4706211.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            38961..39881
                     /gene="gsiC"
                     /locus_tag="ACHJ48_000589"
     CDS             38961..39881
                     /gene="gsiC"
                     /locus_tag="ACHJ48_000589"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO4706212.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIARRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            39884..40795
                     /gene="gsiD"
                     /locus_tag="ACHJ48_000590"
     CDS             39884..40795
                     /gene="gsiD"
                     /locus_tag="ACHJ48_000590"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO4706213.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            40973..43321
                     /gene="pdeI"
                     /locus_tag="ACHJ48_000591"
     CDS             40973..43321
                     /gene="pdeI"
                     /locus_tag="ACHJ48_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4706214.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGQVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGDYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELINTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGLRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            43329..44657
                     /gene="dgcI"
                     /locus_tag="ACHJ48_000592"
     CDS             43329..44657
                     /gene="dgcI"
                     /locus_tag="ACHJ48_000592"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4706215.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(44727..45056)
                     /locus_tag="ACHJ48_000593"
     CDS             complement(44727..45056)
                     /locus_tag="ACHJ48_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4706216.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(45046..45432)
                     /locus_tag="ACHJ48_000594"
     CDS             complement(45046..45432)
                     /locus_tag="ACHJ48_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4706217.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGSKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            complement(45658..46983)
                     /gene="rimO"
                     /locus_tag="ACHJ48_000595"
     CDS             complement(45658..46983)
                     /gene="rimO"
                     /locus_tag="ACHJ48_000595"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO4706218.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            47196..47579
                     /gene="bssR"
                     /locus_tag="ACHJ48_000596"
     CDS             47196..47579
                     /gene="bssR"
                     /locus_tag="ACHJ48_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO4706219.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            47690..48805
                     /gene="yliI"
                     /locus_tag="ACHJ48_000597"
     CDS             47690..48805
                     /gene="yliI"
                     /locus_tag="ACHJ48_000597"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO4706220.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(48802..49428)
                     /gene="gstB"
                     /locus_tag="ACHJ48_000598"
     CDS             complement(48802..49428)
                     /gene="gstB"
                     /locus_tag="ACHJ48_000598"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO4706221.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            49674..50876
                     /gene="dacC"
                     /locus_tag="ACHJ48_000599"
     CDS             49674..50876
                     /gene="dacC"
                     /locus_tag="ACHJ48_000599"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO4706222.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(50923..51681)
                     /gene="deoR"
                     /locus_tag="ACHJ48_000600"
     CDS             complement(50923..51681)
                     /gene="deoR"
                     /locus_tag="ACHJ48_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO4706223.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(51739..52335)
                     /gene="ybjG"
                     /locus_tag="ACHJ48_000601"
     CDS             complement(51739..52335)
                     /gene="ybjG"
                     /locus_tag="ACHJ48_000601"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4706224.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLLVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            52620..53852
                     /gene="mdfA"
                     /locus_tag="ACHJ48_000602"
     CDS             52620..53852
                     /gene="mdfA"
                     /locus_tag="ACHJ48_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO4706225.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(53893..54177)
                     /gene="ybjH"
                     /locus_tag="ACHJ48_000603"
     CDS             complement(53893..54177)
                     /gene="ybjH"
                     /locus_tag="ACHJ48_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO4706226.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(54263..55078)
                     /gene="ybjI"
                     /locus_tag="ACHJ48_000604"
     CDS             complement(54263..55078)
                     /gene="ybjI"
                     /locus_tag="ACHJ48_000604"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO4706227.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(55078..56286)
                     /gene="ybjJ"
                     /locus_tag="ACHJ48_000605"
     CDS             complement(55078..56286)
                     /gene="ybjJ"
                     /locus_tag="ACHJ48_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4706228.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            56370..56906
                     /gene="rcdA"
                     /locus_tag="ACHJ48_000606"
     CDS             56370..56906
                     /gene="rcdA"
                     /locus_tag="ACHJ48_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO4706229.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(57081..58766)
                     /gene="ybjL"
                     /locus_tag="ACHJ48_000607"
     CDS             complement(57081..58766)
                     /gene="ybjL"
                     /locus_tag="ACHJ48_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO4706230.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            59036..59413
                     /gene="ybjM"
                     /locus_tag="ACHJ48_000608"
     CDS             59036..59413
                     /gene="ybjM"
                     /locus_tag="ACHJ48_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO4706231.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(59443..59700)
                     /gene="grxA"
                     /locus_tag="ACHJ48_000609"
     CDS             complement(59443..59700)
                     /gene="grxA"
                     /locus_tag="ACHJ48_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO4706232.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            59860..60147
                     /gene="ybjC"
                     /locus_tag="ACHJ48_000610"
     CDS             59860..60147
                     /gene="ybjC"
                     /locus_tag="ACHJ48_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO4706233.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            60131..60853
                     /gene="nfsA"
                     /locus_tag="ACHJ48_000611"
     CDS             60131..60853
                     /gene="nfsA"
                     /locus_tag="ACHJ48_000611"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO4706234.1"
                     /translation="MTPTIELTCGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            60914..61816
                     /gene="rimK"
                     /locus_tag="ACHJ48_000612"
     CDS             60914..61816
                     /gene="rimK"
                     /locus_tag="ACHJ48_000612"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO4706235.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            61904..62380
                     /gene="ybjN"
                     /locus_tag="ACHJ48_000613"
     CDS             61904..62380
                     /gene="ybjN"
                     /locus_tag="ACHJ48_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO4706236.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            62731..63843
                     /gene="potF"
                     /locus_tag="ACHJ48_000614"
     CDS             62731..63843
                     /gene="potF"
                     /locus_tag="ACHJ48_000614"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO4706237.1"
                     /translation="MTALNKKWLSGLVAGALMGVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            63938..65071
                     /gene="potG"
                     /locus_tag="ACHJ48_000615"
     CDS             63938..65071
                     /gene="potG"
                     /locus_tag="ACHJ48_000615"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO4706238.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            65081..66034
                     /gene="potH"
                     /locus_tag="ACHJ48_000616"
     CDS             65081..66034
                     /gene="potH"
                     /locus_tag="ACHJ48_000616"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO4706239.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTGDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            66031..66876
                     /gene="potI"
                     /locus_tag="ACHJ48_000617"
     CDS             66031..66876
                     /gene="potI"
                     /locus_tag="ACHJ48_000617"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO4706240.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAVISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     CARRG"
     gene            66936..67424
                     /gene="ybjO"
                     /locus_tag="ACHJ48_000618"
     CDS             66936..67424
                     /gene="ybjO"
                     /locus_tag="ACHJ48_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO4706241.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            67465..68592
                     /gene="rlmC"
                     /locus_tag="ACHJ48_000619"
     CDS             67465..68592
                     /gene="rlmC"
                     /locus_tag="ACHJ48_000619"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO4706242.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALVERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIALEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKM"
     gene            68872..70215
                     /locus_tag="ACHJ48_000620"
     CDS             68872..70215
                     /locus_tag="ACHJ48_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO4706243.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            70237..70557
                     /locus_tag="ACHJ48_000621"
     CDS             70237..70557
                     /locus_tag="ACHJ48_000621"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4706244.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHFPH
                     LINQ"
     gene            70569..72050
                     /locus_tag="ACHJ48_000622"
     CDS             70569..72050
                     /locus_tag="ACHJ48_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFO4706245.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCAFIHKNHAEDNWLLQIETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWNPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(72101..72832)
                     /gene="artJ"
                     /locus_tag="ACHJ48_000623"
     CDS             complement(72101..72832)
                     /gene="artJ"
                     /locus_tag="ACHJ48_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO4706246.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNSRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQQISDQWFPQ"
     gene            complement(73123..73791)
                     /gene="artM"
                     /locus_tag="ACHJ48_000624"
     CDS             complement(73123..73791)
                     /gene="artM"
                     /locus_tag="ACHJ48_000624"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO4706247.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(73791..74507)
                     /gene="artQ"
                     /locus_tag="ACHJ48_000625"
     CDS             complement(73791..74507)
                     /gene="artQ"
                     /locus_tag="ACHJ48_000625"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO4706248.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGIVEIPVQMQIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(74514..75245)
                     /gene="artI"
                     /locus_tag="ACHJ48_000626"
     CDS             complement(74514..75245)
                     /gene="artI"
                     /locus_tag="ACHJ48_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO4706249.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITAEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(75263..75991)
                     /gene="artP"
                     /locus_tag="ACHJ48_000627"
     CDS             complement(75263..75991)
                     /gene="artP"
                     /locus_tag="ACHJ48_000627"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO4706250.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(76209..76724)
                     /gene="ybjP"
                     /locus_tag="ACHJ48_000628"
     CDS             complement(76209..76724)
                     /gene="ybjP"
                     /locus_tag="ACHJ48_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4706251.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYHIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT        18795 a        18761 c        20253 g        19372 t
ORIGIN      
        1 ttatctttca ttatctgaaa taaaacaatt cgttactgaa gaaattaaat tgaatttcaa
       61 tgagcagcaa ggattgttag ataaagataa taaatgttat atcctgttat catccgataa
      121 ctctgggcga gtaatgcgct tatcgcacca ggcgttaatc tcaatgcttg agccagaggt
      181 taagaaaaag actatctgga ataattactc tatttatcca tctttgcagg atacccatga
      241 agttgtcaga gatgatccag aaacgatttg tatgcgtgca tttcctttat ttgcaaaagg
      301 atgggaatat gcgcagaaaa ataagaaaca tcaattgatc ctcaatgctc ttggattcaa
      361 aggttatatt cgtgatatat ttatgtcagc aataatgcga aaaacagatt ttgtgctcga
      421 atgcaataat caaccgacag aacttaattc atcattttcc tctttgatga acgattcaga
      481 tcaatggcaa cagcatactc tgaaagataa acattacgct aatctattaa cgatgctgga
      541 tctcaatgat gccagtgaaa gtgataagtc taaaatattt ttctgcctgt ctgccgtatt
      601 tgcaaatatt tctcacagta atgtctttaa cggtattcct gatgcgtcaa aaactttaaa
      661 acgttatgca tttgctttat tggcgaaagc tcattcttta gatgaaagta tgataagcaa
      721 tcaaactttt aatacataca aaacggtatt gcttgatttc aataatctgt ctaatgaaga
      781 ggccaaccag ttgcgcataa gttcgttata tcgtgatatg gtccgctatg cacaatatcg
      841 tttttctaaa gttttatccg agtggacgcc agacgcatgg ctgtaaagat cgcagcagaa
      901 taatggcatt gatggcccag gctcaggcca gggtcggcaa taccggttcc tgcgaagcga
      961 tgacggcggc aattgtaacc acgaaatgcg gtatcacttg ctgttttctg ccctgaagac
     1021 tggcgctcac tgtgccgtcg aattttcaat caaatatatt gcgagagggc gtgtttgcga
     1081 gccgctttcc agaaacagaa aaaccattac ccctgaaaac cgaaaaatgc cacaatattg
     1141 gctgtttata cagtatttca ggttttctca tggcattaac cgccgcgctt aaagcgcaaa
     1201 ttgccgcctg gtataaggcg cttcaggaac agatccccga ctttattccc cgtgcgccgc
     1261 agcggcagat gattgcggac gtcgccaaaa cgctggccgg agaagaaggg cggcatctgg
     1321 cgattgaagc ccccactggc gttgggaaaa cgctctccta tttgattccc ggcatcgcca
     1381 ttgcccgcga agagcaaaaa acgctggtgg tgagtaccgc caacgtggca ttgcaggatc
     1441 agatctacag caaagatttg ccactgctga aaaagatcat tcccgatctt aaattcactg
     1501 ccgcttttgg gcgtgggcgc tacgtttgcc cgcgtaatct gacggcgctt gccagcactg
     1561 agcccacgca acaggatctg ctggcgtttc ttgacgacga actgacgcca aataatcagg
     1621 aagagcaaaa acgttgtgcg aagctgaagg gcgatctcga cacttataaa tgggatggtc
     1681 tgcgcgatca tacggatatc gctatagatg acgatctctg gcgtcgttta agtaccgaca
     1741 aagccagctg cctcaaccgc aactgttact actatcgcga atgcccgttt tttgtcgctc
     1801 gtcgggaaat tcaggaagcg gaagtggtgg tggcaaacca tgcgctggtg atggcggcga
     1861 tggaaagcga agccgtattg cctgacccga aaaatttact gctggtgctg gacgaaggcc
     1921 atcacctgcc agatgtggcg cgggatgcgc tggagatgag tgccgaaatc accgcgccat
     1981 ggtatcggct acagctggac ttgttcacga aactggtcgc tacctgcatg gagcagtttc
     2041 gcccgaagac catcccgccg ttggcgatcc ctgaacgttt gaatgcgcat tgtgaagagc
     2101 tgtatgagct tatcgcctca ttaaacaaca ttctcaatct ctacatgcct gccgggcagg
     2161 aagcagaaca ccgttttgcg atgggcgaac tgcctgatga agtgctggag atctgccagc
     2221 ggctggcaaa actcaccgag atgctgcgtg gtctggcgga gttatttctt aacgatttaa
     2281 gtgagaaaac cggcagccat gacattgtac gtctgcatcg gttgattttg cagatgaacc
     2341 gcgcgttggg gatgttcgag gcgcaaagca aactctggcg gctggcttcg ctggcgcaat
     2401 cttccggtgc accggtgacc aaatgggcga cgcgggaaga gcgcgaaggg cagctacacc
     2461 tctggtttca ctgcgtggga atacgtgtca gcgatcagct ggaaaggctg ctgtggcgca
     2521 gtattccgca cattattgtc acctccgcaa ccttgcgttc gctgaacagt ttttcgcgtt
     2581 tgcaggagat gagcgggctg aaagagaaag cgggcgaccg ttttgtggcg ctggattccc
     2641 cctttaacca ctgcgaacag ggcaaaattg ttattccccg gatgcgcgtt gagccttcca
     2701 tcgacaacga agaacagcat attgctgaaa tggcggcctt tttccgtgag caggtggaga
     2761 gcaaaaaaca tctcggtatg ttggtgctgt ttgccagcgg gcgtgcgatg cagcgctttc
     2821 tcgactatgt gacggattta cgtctgatgt tgctggtgca gggcgatcag ccgcgttacc
     2881 gtttagttga actgcaccgc aaacgcgtcg ccaacggtga gcgtagtgtg ctggtgggct
     2941 tacagtcatt tgccgaaggg cttgatttga aaggtgatat gctcagccag gtgcatatcc
     3001 acaaaatcgc ttttccgcct atcgacagcc cggtggtgat cactgaaggg gaatggctga
     3061 aaagcctcaa ccgctatcca tttgaggtgc aaagcctgcc gagcgcctcg tttaacctga
     3121 ttcagcaggt tgggcgactg attcgaagtc acggttgctg gggcgaagtg gtgatttacg
     3181 ataaacgctt gctgaccaaa aattatggca agcgactact ggatgcatta ccggtatttc
     3241 cgatagagca accggaagtc cctgaaggta tagttaaaaa gaaagaaaaa acgaaatccc
     3301 cacgccgtcg gcggcgttaa tgatgtgagt caggtaagga gtcgtaaatg gactatcgca
     3361 aaatcattaa agagatcggg cgcgggaaaa accacgcgcg cgatttagac cgggatactg
     3421 cgcgcggtct gtatgcccat atgctcaatg gtgaagtccc tgaccttgag ttgggcggtg
     3481 tattgattgc gctgcgtatc aaaggggaag gggaagcaga gatgctcggc ttttacgaag
     3541 ccatgcaaaa tcacaccatc aagctgacgc cgcctgcagg caagccgatg ccgattgtca
     3601 tccccagtta caacggcgcg cgtaaacagg ccaacctgac gccgttgttg gcgattctcc
     3661 tgcacaaact cggttttcct gtggtggttc acggggttag cgaagatcca actcgcgtgc
     3721 tgaccgaaac cattttcgaa ctgatgggta ttacgccaac gttgcacggc gggcaggcgc
     3781 aggcgaagct cgacgaacat caaccggtgt ttatgcctgt cggcgcgttt tgcccgccgc
     3841 tggaaaaaca actggcgatg cgctggcgga tgggcgtgcg taacagtgca cataccctgg
     3901 cgaaactggc gacgccattt gccgaaggtg aggcactgcg tctttccagc gtttcgcatc
     3961 cggaatacat cgggcgagtc gcgaagttct ttagcgatat cggcgggcgg gcgttactaa
     4021 tgcatggtac tgaaggcgaa gtgtatgcta atccgcagcg ctgcccacag atcaatctca
     4081 ttgatcgtga agagatgcgg gtgctgtatg aaaaacagga ccctgctgga agcgagttac
     4141 tgccacaagc aaaagatccg gaaaccacgg cgcagtggat tcagcgttgc cttgctggcc
     4201 gcgaaccgat tcccgaatcg ctgaaaatcc agatggcttg ctgcctggtg gctacgggtg
     4261 aagcggcaac tatcagcgat ggcctggcgc gcgttaatca ggcattttaa ttcttttctc
     4321 agccggatga gccatgtcat ccggctttta ccccaccgca atatgaaatt cctgcatctt
     4381 tattgacctt cccgcgcccg gcgtgcagca taaaaaaaca acaaacacat aacataaaca
     4441 ggagttaacc atggaaagtg gtcatcgctt tgatgctcag acgctgcaca gctttattca
     4501 ggctgtattt cgtcagatgg gtagcgagga acaagaagcg aaattagttg ccgatcattt
     4561 aatcgcggca aacctggcag ggcatgattc acatggtatt ggcatgatcc caagctatgt
     4621 acgctcctgg agtcaggggc acctgcaaat taaccatcat gccaaaatcg ttaaagaggc
     4681 gggggcggcg gtcacgctcg atggcgatcg cgcatttggt caggtcgcgg cacatgaagc
     4741 gatggcgctg gggattgaga aagcgcatca gcacggtatt gctgccgtgg cgctacataa
     4801 ctcgcatcat atcggccgta tcggttactg ggcggagcag tgtgcagcgg cggggtttgt
     4861 ctctatccac tttgttagcg tggtcggtat tccaatggtc gcaccgttcc acggtcgcga
     4921 cagccgcttt ggcacgaatc cgttctgtgt ggttttccca cgtaaagatg attttccgct
     4981 gttgcttgat tacgccacca gcgccattgc atttggcaaa acccgcgtcg cctggcataa
     5041 aggcgtcccc gtgccgccag gttgcctgat tgacgttaac ggcgtgccga cgaccaatcc
     5101 ggcggtaatg caggagtcgc cgttgggttc gctgttgacc tttgccgaac ataaaggcta
     5161 cgccctggcg gcaatgtgtg aaattcttgg cggggcgctt tctggcggta aaacgacgca
     5221 tcaggaaacg ttacaaacca gtcccgatgc cattcttaac tgcatgacca ctatcatcat
     5281 caacccggaa ctgttcggcg cgccggattg tagcgcgcag accgaagcct ttgccgagtg
     5341 ggtgaaagcc tcgccgcatg atgacgataa gccgattttg ctcccgggcg agtgggaagt
     5401 gaacacgcgt cgcgaacggc aggagcaggg gattccattg gatgcgggaa gctggcaggc
     5461 catttgtgat gcggcgcggc agattggtat gccggaagag acgttgcagg ctttctgtca
     5521 gcagttagcc agctaaaaaa aagcccgtcc agtggcggac gggcaaacaa gggtaacata
     5581 ggatcaatga gggttagagc atatgcgtct gtcggcaaac agacagggaa atacttgtgc
     5641 aggacgtagc gtaaacgcct gatccgtcca acggttcgga ttttgtaggc ctgataagac
     5701 gcggtagcgt cgcatcaggc atctgttgcc ggatgcggcg taaacgcctt atccgtccta
     5761 cgaattactt ataaattacc gcagtaccgc ttaatttatt gttgttggtg gcggaagtga
     5821 tgctatatcc actggcaccg gctgccgccg ctttctcagc cagtttcgct tccagggcat
     5881 cgagggtgga tgcgccatcg gcagaaacca cgccgatttt attcatgttc tgtgcctggg
     5941 atgccgttac cggttccgcg gcaaacacgc caaatgacag agctgaaaga gccatagcag
     6001 caacaacagt attgatagtt ttcataatta atctctcgca ggtgattttt gtataagtga
     6061 cgttgtttcg tcgatgtgat gagtatcacg tttttttacg agagataaaa tcgaagagaa
     6121 ttgaccgcct tgttcaaata aattgattga tatctaacat actgataaac catttatttg
     6181 ttgtacttat ggacttgttt tatgcagcgt ggcagtcacg acagcgttaa aggcacggaa
     6241 tgccacgcaa aatacaaatt atactcgcta tacatctcgc cagttgtagt taacgtaact
     6301 ggctttcttt cgaacctctg cgattatatc ctgtataagc tggtttttgt aaatctttct
     6361 cctgctgaca atgaatacat aagcgcacgc caggaatggc ttcccgacgg gcttgcggga
     6421 tgggggcacc gcactcttca cattcataca ggctttcgcc acgcggaatt tcaccccgag
     6481 cgcgggcaat cgcatcttca attgtactgt tgatctgttc gttgacggcg tcatcgttag
     6541 cccaaccgga tgccatatcg tcctccccat atatcaatgc ttgtatagtt aagtgtagct
     6601 aatataggga cgaacctggg cagttcaagc gtcagatctc cgaccattcc cgcagcagat
     6661 tatgataaag attgagcagc gacaggatct cttcattttc accatagcgg cttttcagcg
     6721 actgaatatt ctgaatattt ttgtccagtt caaacagcat ggcgcgcttt ttatcatcgc
     6781 ggatcatcga ctggatccat ataaatgatg ccactcgtac gccgcgggtt acgggtgtca
     6841 cgcaatgcag gctgctggag ggatacaaca ccagatcacc tgccgggagt tttacccgat
     6901 gttgtccgaa ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt ggatcgctta
     6961 aaaacagcgt ggcagaaagg tcagtgcgca tccagccgtt ttgtggatgg ctgcgtactg
     7021 cgccatccac atgaaaacca taggtttcat tgttctgata gcgattaaac agcggcgtgg
     7081 aaagggtacg cggcaaggcc gcggcaaaga acaaagcatg ttggttaacc gcgttcagca
     7141 cctcattttg cagggcggcg tataacgtgc tgcgggtgtc tacctgttga ttgttcttaa
     7201 cttgcgcacc ttgtgcgccg gtggtgacgc gtccatccac ccattcggct tgttccagtt
     7261 gttcgcgaaa acgggcgacg tcctgtggcg ataacacgcc gggaatgtgg tacatcatac
     7321 aagttctcca aaaagtgggg cctgcgcccc acatctgaat cagaaatgca tattggctgt
     7381 gagcaagaag gttcttggct cgcccgggtg ataacggtag ccgctcttat tgattgaggc
     7441 gacgtaatcg gtatcaaaca ggttgtagac gtttagctgg aagtcgagat tgcgattaac
     7501 cctatacccc agtttggcat cggcgaccca gtaaccttcg gtaaacgctg gcgttcccac
     7561 cgcgccgtct gaacctttat gcatactgcc gatatagcgt gcgcccgcgc caacagagat
     7621 atcatcggtt gcctgatatt ggctccataa ggtgaaggcg tgctccgggg tatacggcag
     7681 cgatgaggaa ccatcctggg caacatcttt gccgtttttg atggttgctt tttgctgggt
     7741 atagccgcca atcacctgcc acgcgggagt gatattccct gccacggata tctcatagcc
     7801 ttcgacgcgt ttcttaccgt attgcgagta agtcccgtca tcattttgct caacttcatt
     7861 ttcgatatca gtacggaaca gcgcggcggt gagcaacagg cgtttatcca gaacctgcca
     7921 tttggtgcca atctcgctgg tgttggcttt ttgcggttta aaatcggtgc ggttggcact
     7981 gttaccgctg ccagactgcg caagggcgaa gttgttgccg cccggaggct gctgggaaac
     8041 ggcatagtta atatagacat tgccgttttc cgtcaggtga tacagcgccc cggctttcca
     8101 gttcaccaga ttgcccgact tggcggtgtc gacggtggtg accggagaac cttttgccac
     8161 accagctggg caggtgatgg caccgcgtcc gctgccgccg caggcggtgg cactgtcata
     8221 ttcagtatga taattatcca gacggatccc gccgttcagc tcaaaatcac gggtgatttg
     8281 cagcgtatcg aatgcgtaaa ttgcgaaggt atccgtctga ccattggcgt ttgcgccgtt
     8341 gcgcgtcagg ccgccgggat gaatgctgct gtcaggatga taaatattta ccgcaggtaa
     8401 cgtcaccgga ttaacgccgt agttagtctg cgtttcacgg gtaaattcca cgccggtact
     8461 gacatcatga ccgatagaag cggtatagaa cgtcgaggtc aggttggtct ggttggtgag
     8521 aattttatta ctcacatctt tggtattcgc cgtgcgtgac cacgtccagc tattcacatc
     8581 gctggtgggc tgggtaatat tcgacgcccc acccataatc gccgtcatca ggtaatcctg
     8641 cttcacgcgc gaccaacggg tagtgttgcg aatggtggtg ttatcgttga tatcgtgctc
     8701 aaaacgcatg gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg tgccgtaaaa
     8761 gttatgcgta tcaacttttc cggaatgatt cagggtcgcc gttcctgcag atggggcaga
     8821 atagcccggc aaaccgatgg ttggaatgcc gccgtctggc gtgttgtgct gggtgacatg
     8881 cagataatta agatacaaac gattcgctgt accaaggcca aaagcgatag aaggggcgac
     8941 gccgtaacgc tcatttttga ctttgtcgcg tccggcatcg tgcgtttttt cgcccatcac
     9001 attcaggcgc accgcagtgg tatcaccaat gacctgattg acgtccagcg tgccgcggcg
     9061 gaaccaggcg ctgccaatac tggcggaggc gtcaatgccg gaatcattgc gcggctgctt
     9121 gctgatcata ttgatcgagc ccgtcggcgc gctgcgcccg tagtcggtgc cggaaggccc
     9181 tttaatcact tcgacctgct cagtattgaa ggtgtcgcgc gagacgctgc cgatatcgcg
     9241 aatgccatca atataaatac tgttagaggt atcagcaccg cgcatataaa tggcgtcgcc
     9301 agtggtggag ttaccgttct cacccgcaaa aaacgcaccc acgccgggga cgtttttgag
     9361 cgcatcggta aggttggttg cgccctgatc tttaatcact tgttcagaaa tcaccgtcat
     9421 cgtgcgggta gtatccgcta ccggacgcga gaatttcgga tcggcagatt gttgtggcgc
     9481 ataaagcgaa ggcgttgatg cttcgacaac cagcgtgtca tccgcgttag tttgcccttc
     9541 ggcggcgagt gcctgagcca caggcgtgat gccaatacaa agaccggcaa agaacgtgag
     9601 cgaatgaaat tgtctggcag ggaaattgcg attgttttcc attttgcagg tgactttttc
     9661 ttatatgttg aaattttcac tgccacgaga aagcctctct gcggatgcga tgccactgtt
     9721 aatctcgtga tagtgttcgt gcaagtgata ataattttga taagcattat cacttggcgc
     9781 gattttctat cacccattgc gcgaaaaaga aatacattta tttacattgc gttcatattt
     9841 gatgtttttt gattacaaaa caggcgaaaa ctgcatccag aggcgaatgc agttacgttg
     9901 gtggggttat ttgtagatgg ttgcagtacc gaacatctgg tcatttccgc ctgcggaatt
     9961 aatgacataa cctttcgcgc cttgctcgcg cgctttttct gccagtttat cctccagatc
    10021 gcttaggttt gatgcgccag ttgccgaaac ggtaccggcg ggccgtagct gaccgctgtc
    10081 caggttactc atcggttgcg cggcataagc cgtgagagag attccgctca ggacggtggc
    10141 aatcagtagt gtgaggcact ttttcataat gacatcctca taaatacaac gggtagtgcc
    10201 tgataagtgt aggtcgcctg cgcgatgcaa atgacgcaaa aaattgacag agatgtgctt
    10261 attttttgaa cgatatattt ttacaaaata agacaaatca atgacatgca taatgctgat
    10321 ttttatgatg aattgccata aaccatagcc atgttaaggt tttggggaat gataaacgca
    10381 gccagatatt taccctgtcg cgttatgcgg gtatcttacg ccgctgctta aaggagaatg
    10441 ctatgtccgc ccagaaaccg gggttgcatc cgcgcaaccg tcatcacagc cgctacgatc
    10501 tcgccacgct ttgtcaggtc aatcctgaac tcaagcaatt cctcacgctt acacccgccg
    10561 gggagcaaag cgtagacttt gccaatccgc tggcggtgaa ggcgctcaat aaggcgttgc
    10621 tggcccattt ttacgccgta gcgaactggg atatcccgga cggttttctc tgcccgccag
    10681 taccgggccg ggcggattat attcatcacc ttgccgattt actggctgaa gcaagcggaa
    10741 caattccagc taatgccagc attcttgata tcggcgttgg tgcgaactgt atttatccgc
    10801 tgattggcgt acatgaatat ggctggcgtt ttaccggtag tgaaaccagc agccaggcgt
    10861 taagcagcgc gcaggcgatc atcagtgcta atccggggct taatcgcgcc attcgtctgc
    10921 gtcggcaaaa agagagtggg gcgattttta acggcatcat ccataaaaac gagcaatacg
    10981 acgcgacctt gtgtaacccg ccattccacg attccgccgc tgcggcgcgg gcaggtagtg
    11041 agtgtaaacg ccgtaacctg gggctgaaca aagacgatgc actgaacttt ggcggccagc
    11101 aacaggagtt gtggtgtgaa ggcggtgagg tcacctttat caaaaagatg attgaagaga
    11161 gcaaaggctt cgcgaagcag gtgatgtggt ttacttcact ggtatctcgt ggtgaaaact
    11221 taccgccgtt gtatcgtgcc ctgacggacg tgggcgcggt gaaggtggtt aaaaaagaga
    11281 tggcccaggg gcaaaagcag agtcgcttta ttgcctggac ctttatgaac gacgagcagc
    11341 gccgccgttt tgtcaatcgc cagcgttaaa gcgttggctc ccccggcggt aacggttcag
    11401 ccggggtcgc gcccggagca ggcagcacct gataagtctg caccggcgcg cgaacgcccg
    11461 ccaggtcgaa atgttttttc acctggctgt cgagggcaaa gcgtaccgtc cactgtttga
    11521 gtggcagcgt ggtgaacgaa acacgcagtg taaacgcggt attgcttaag ccaacaatcc
    11581 cggcaaagtt cggttcacca ataatcagcc cacgaatttc ttcgttttcc attaattccg
    11641 ctaccgcatc tttcagtgcc tgattggctt tatcagcatc ttcatggcga tcaacatcgt
    11701 agtttgccac taccgaacca atgccgcgga caaagttggc aaaggtggta atcgaagacc
    11761 acggaatgat gtggtacgcc ccggtatcct ggcgtacgcc cacggagcga atcgacatcc
    11821 gttccacagt gccggtcaac ggcccaatag tcaccaaatc tccagtgttc atgccgtttt
    11881 caaactgaat aaataccccg gtgataatat ctttcaccag cgtttgcgaa ccaaacgaga
    11941 tagccagacc taatgccccg gcacctgcca gcaatggcgc gatattgacg ccgatttccg
    12001 acaacacaat catgatggtg atggtactga taatcaccgc cagcgcgtta cgaaacagcg
    12061 taagcagggt acgcgtgcgg gcgctgggta gcgggcggcc atgaatatcc gaagccagcc
    12121 ggttttcgat caaactggcg agcaccgtcc agccaaccgc cgagaagaaa agaatgagtg
    12181 cgatacggat caggatatct acggttttct gccccgcgcc gttttgcagc cagttccaga
    12241 aatcgaacaa tccccatgcg ctcaacagca gcattaccgc cacgcagacg gtcagaatac
    12301 gcgccgtttt cagcgccgcc gaaagccagc cattcagccg tttttgcaac tccggatagt
    12361 tacgctgagt atgtggcgag agagtgatgg tttttgccag ccagcgggaa aacataccgg
    12421 aaacaaatgc tgcgatacca ataatcgcca ggctgcgcac tgttgcaccc atcataaatt
    12481 tcaggctgtt gcccggatcg aacaacgaaa agaaaaacag cacgataaaa taggcgcttg
    12541 ccagccagtg ccacaccagc gcaaaggcgc ggataaacag gctgaaaaag gccagcgaat
    12601 gctccgcaaa gttgagcaaa tgctgggtaa tctctttttt attacgaaag atcaggtaca
    12661 acgcccagac ggtcatgcac agcataatga tgacgttcgc cagcgcacct atctgtacat
    12721 tcacctgatt agagataatc ggcacggcca caatcaggcc ataacctatc aaactgccta
    12781 accagctcag gcgacgactc cagtaacggg cgctctcgtc ctgaatcgtg aacgggcgca
    12841 gttccgccac gtttgggcaa aaaatcaggc gtagtacggc tttgaaaaat tcaatcaggg
    12901 caaaggcgtt gagaaacaaa ctttgttgga aagcgatggt gcgactgcct gcattcaggt
    12961 tgtcgcttaa tatctggccg acaaataatg tcagtgccag taacagcagg tcgataataa
    13021 acgccccgat aatcatcgcc ggaagctgca gccagttact gcgctcacga tttttttgcc
    13081 gcgcccactg acccattttg cgatacagcg gcagtgcgca caggcgaatc agccagtaaa
    13141 aaccaaacac taaaaccgct aacattgaaa aatgggtcag cgcattgctg aaggtttgtg
    13201 gattaaacgg cttatgcggg gagccggtga tattgcgata aagctgcccg aagcgggcgg
    13261 aaagggcttc accataatgg cggctgaatt cggtgacctt ttgcagcacg gtttgctctt
    13321 caaccagcgt cggcggcacg atcttcggga ccggttcagc aggtggcgtc gcggcgacgg
    13381 tgcgcaactg gtcgatcaac tctttacgcg aggtgtcatt atccagcaca tccgccagtg
    13441 cgccataagc cgcttttttt tgttcgatat ccggttccgg ggccggttca gtcgttgagt
    13501 ccgttgtcgt tgtggttgta acgccgggta tggataccgc gtgggcaggt gcgcccagca
    13561 ggcagaagag gatgaacagg atccaccgca tgactcctcc agtgagaaaa cagagcaaaa
    13621 agataagtat agatgctggg ggaggggtga ttttaaatga gaggaatctg gtgtacctcc
    13681 ctttcgggtg aaagggaggc agagaagtta agagacgtgc tgtaaaaatt cctgcaagcg
    13741 ctggctcggc ggatttttaa tcaacacttg tggatcgcca tcttccgcaa tccggccttt
    13801 gtcgataaag atcaggcgcg aagctacttt ctcggcaaaa ccgatttcgt gggtcacgat
    13861 caccatcgtc atcccttctt cagccagatc ttgcataacc ttcagcactt catggcgcag
    13921 ttccgggtca agagcggaag tcggttcatc aaacagcatc attttcggct tcaccgccag
    13981 cgcgcgggca atcgccacac gctgctgttg accaccagaa agttcggaag ggtagtgatg
    14041 tgcacgttct gccagaccga ctttcgccag cagctcacgt gccagttttt ccgcctcttc
    14101 tttgttcgcg ccacgcacgc gtagcgggcc aaacatgacg ttttccagcg ccgtcagatg
    14161 cgggaagagg taaaactgct ggaacaccat acctgcttcc tggcgaatca ggcgctcgtc
    14221 aactttcgga tcgttaacct tcaggccatc gacaatcaga tcgccggagg tgatttcttc
    14281 cagtttgttg atgcagcgca gcagggtcga tttaccggaa ccggacggcc cgataatcac
    14341 cacgacttcg ccctgggcaa tgttcaaatc gatattgtgc agcacctggg ttgggccaaa
    14401 gtgcttggag acgtttttaa attcaatcac aggattttca tccttctttc cagacgacgc
    14461 agaataaagc tcagcaccag ggtaataatc agatagaaca ccgccacggc gctccagatc
    14521 tcaagggcgc ggaagttacc ggcaataatt tcttgcccct gacgggtcag ttccgccacg
    14581 ccgatcacaa taaacagcga ggtgtcttta atgctgatga tccactggtt acccagcggc
    14641 ggcagcatac gacgcagtgc cagcggtaaa atgacgtagc gaatggtttc ccaacgtgaa
    14701 agaccgagcg ccagtcctgc ttcacgaaaa cctttgtgga tagacagcac cgcaccacgc
    14761 gtgatttccg caatgtacgc gccggagttg atcatgatgg tgaccaccgc cgcagtaaat
    14821 gggtcgatgc gtaagtcatt aaacgccatc ggcagggcaa aatagataaa catcacctgg
    14881 acgacgatag gtgtgccgcg gatcacttca ataaaaacca gcgcgacgtg gttggctatc
    14941 caaccaccga aggtgcgtgc aaaacctgcc agcaatccga tcaccaggcc gccagccaga
    15001 ccgaggaccg atatccacag ggtcattttg gcaccttcaa tcagaagcgg aatggcaggc
    15061 cagatagcac tccagtcaaa ctgcatatgt tgttcctgtt accgtggtgt aaataatcaa
    15121 attcagggta attaatggcc ccggagcggg tttgcgccct caccctaatc ctctccccag
    15181 aggggagagg gaactgccag tgcgctttac aggtgtagcg ttattatttc ggttcagtac
    15241 cgaaccattt tttgtagatt tcgttgtaag ttccgttctc gcgcagggtt ttcaacgcgc
    15301 cgttgacttt gtcacgcagc tcgtcgctac ctttcgggaa cgcaataccg tattgctgcg
    15361 cttccagaga gtcgcctacc gctttgaact gaccgttacc ggcggttttg atgaagtaca
    15421 gaatgtttgg cgtatcgtgc agaacggcgt ctgcgcggtt ggtgcccagt tccatatagg
    15481 cgttatcgat gttcgggaac tgacgcagat ctttagtttt gatgtttgct ttcgcgtaat
    15541 caacggagcc agtgccgctc ttcaccgcaa ccactttccc gtcgagatct ttcacgcttt
    15601 tcacatcgtt attgttagct ttcaccatca ctaacaggcc gcttttgtag tagccgtcag
    15661 agaaatcgat cgctttttta cgctcgtcgg tgatggtaat gcccgccagc gccagatcga
    15721 cgtttttggt ttgcagtgcc ggaatgatcc cactgaaatc catcggcttc agttcgtaat
    15781 ccagcttcag ctctttagcg atggcagccc acagatcaac gtcaaagccc acatatttat
    15841 cgccctgttt aaattcaaac ggaacgaagg cggtatccgt cgcgacaact aattttttat
    15901 ccgcggcatg agaagaaacc gcaaaagcca gggtcagtgc agccagtgaa acttttaata
    15961 cagacttcat agcatttcct tttttctatc cacgggacga tcccctgcgt gacattcaca
    16021 tatatgaaaa aatcgtgcca gttttgcaac tcattgattt ttcgagatgc ggcaaaaacg
    16081 tgatgcacga tttatagggc aacaccctga agatgcacca ttctggggca ccaaactggt
    16141 gcgctaaaat tgtgcactca acacagtgat tatttagcgt aaaacctgat gaaaaaacaa
    16201 tctttatgta acgattgtgt gatgatgtgg atacaaaaaa tttacttaat cagctgggga
    16261 tagcagatgg atgcactaaa taagtgcgtt gaggcgggct gcaaaacaaa acggcctcct
    16321 gtcaggaagc cgcttttatc gggtactaaa attctgcacc atcagcgatg gatttattcg
    16381 atgttagact cgataaacca caggaattta tccaggtcgc gagacgcggc ggtcaggata
    16441 tctgcggtgt cgtcatcttt cgcttcgcca atcgctttgc gtacgtcatt agcgacgatt
    16501 gcgtaacggt cagccagttc tttcaggtga tcctgaacgt tgtggatgtc cagcgggtaa
    16561 cttttcagcg gggttttgct gttgataact tgagtggtcc ccagagctac accgcccagc
    16621 tgcactgcac gttctgccat ggtatccaga tgatcgatca gtgcagtgcg gaagccatcc
    16681 agcatttcat gtacggcaat gaagttagcg ccgcgcatgt tccagtgcgc ttgtttggta
    16741 atcaaagaaa gatcaataaa ctggataacc tggcgattca gcaactctac tgttgctttt
    16801 ttctcgctgt cagagacatc gttgcgggta taaagcagat tggtcgcttt tgatttaact
    16861 aatttagcgg tactcataat ttcatatcct cttgatgtta tgtcccagta attaacgaga
    16921 ctaagtatag caccggctat gtgttccgct attctggctg ttcctatcac actaatagtg
    16981 gtaacaagcg tgaaaaacaa aactaataaa gattcaatga gttagatata ttgataagaa
    17041 caattcttat ttatttgctc gccaggaatt ttcggaataa atatgctttt gcataattcg
    17101 atatataaag acggtgtaga ggaaaagtag cgagaaattc tgcatggtta tgcataacca
    17161 tgcagaaatg taaatttaat taatgtctaa ttcttttatt ttgctctctt tgcgtactgt
    17221 cagcgtagaa cccattgaag cggcgataat tgcgccgagc gccagcagct gtatgggtgt
    17281 cagtgtttct ccgaggaaaa tcatcccgga aacggcagcc agcgccggtt ccatgctcat
    17341 cagcgtacca aatgtccgtg ttggcaaacg ggtgagggca atcatttcca gcgaataagg
    17401 cagagcggtc gagagaatag cgaccgccag acccaatgga ataaccgacc agtgccagag
    17461 tgcttcacca gcctgaagcg ctccaattgg cacgaaaatt aacgctgcaa tcaacgaacc
    17521 aattgccacc gtcgcagggc catgttccgc tcctgcgcgt tgcccactta aaatgtaaat
    17581 agcccaacaa gccccggccc ccagtgccag cgcacagccg gttaaatcga catgggaaac
    17641 gtcttgcccc agcggtagca ggaaccacag accaagaacc gccagcacaa cccagacgaa
    17701 atctaccggg cgacgagaag agaacagcgc caccgccagt ggtccggtga actccagcgc
    17761 caccgcaata cccagcggta ccgtctgaat agagagataa aaaagataat tcatcccacc
    17821 cagcgaaacg ccgtaaaaca acagcggtaa ccgttgctct ttggcaaagc gcagtcgcca
    17881 tggcttaaag aacgcgatga ggatcagcgt tcctaatgcc aggcgcagcg cagtgacacc
    17941 cggtgcgccc accagaggaa aaagtgactt agctaacgaa gctccacctt gaatagacgc
    18001 catggcaacg agcaatatga ctattggtaa ccagaccggc attttacgta atgaaccagg
    18061 catcctttct cccacaaata tctagactta agtaaagcgt ggagtgtact ggatataccc
    18121 aatgctggtt gagcatttgt tgaaaaaatt ttcccccgtt ttgactaaaa tgcgccagga
    18181 ttgatggaat cattagtctg gtgattagga ataatctgga tgaatgacag ggaaaacatg
    18241 cgtaatactt acgcagttct ctgaaaaagt gatttaaatt tagatggata gcggtgtatg
    18301 gaaacgttct gttacatgaa atggcccgtt agacatcaca aatcgcgaag agtttcccat
    18361 taatttttga tatatttaaa acttaggact tatttgaatc acatttgagg tggttatgaa
    18421 aaaaattgca tgtctttcag cactggccgc agttctggct ttcaccgcag gtacttccgt
    18481 agctgcgact tctaccgtaa ctggcggtta cgcacagagc gacgctcagg gccaaatgaa
    18541 caaaatgggc ggtttcaacc tgaaataccg ctatgaagaa gacaacagcc cgctgggtgt
    18601 gatcggttca ttcacttaca ccgagaaaag ccgtactgca agctctggtg actacaacaa
    18661 aaaccagtac tacggcatca ctgctggtcc ggcttaccgc attaacgact gggcaagcat
    18721 ctacggtgta gtgggtgtgg gttatggtaa attccagacc actgaatacc cgacctacaa
    18781 acacgacacc agcgactacg gtttctccta cggtgctggt ctgcagttca acccgatgga
    18841 aaacgttgct ctggacttct cttacgagca gagccgtatt cgtagcgttg acgtaggcac
    18901 ctggattgcc ggtgttggtt accgcttcta atcactttgg tgatataaaa aatccgcctc
    18961 tcggggcgga tttttgtttt taaggcttcg ggtcgaaaat atcggttccg agatggttgt
    19021 aatccacctt ctgtaactgg aagttggtga tgtatatcgg cccggctttc ttctcagata
    19081 taaacgggta tttgatgtta atttccttcg ctttaattcc cgtccactgc gagaaaaagc
    19141 ctaagaagtc attggctgag cggcgggctt taatcacccg atgcgcttta tcgtcgctgg
    19201 aaatgaccat aaaaggcacc tggaaatttt gctgatactt atcatcatgg gcaaggtatt
    19261 gcacgtcttt gccgcgctct ttaaaggcca gaccgtggtc agaaaagtaa accagcgaga
    19321 agctgctgcc gctgttgcgt aactgatcgt acagcttgcg cagtaaatcg tccgtttgcg
    19381 tcatagtata gagatagcac gacgtttctt tcgattgcac aaaggtttcg tatttgcctt
    19441 ttgtccggtc gcaggcctgc ggatgcgagc ccatcagatg cagaacaatc agctgcggtt
    19501 gcgagtgctc ttgcgccagc acttgagcgg tcatatccag taacgcttcg tctttcgtgt
    19561 ttttatctgc ttcaaaatta ccttctttca ggaagtacac ttcatctgca cgtttggcga
    19621 tgctggcgat agcagtatcg tattcgccga tttggccctg gttggaaaac caccaggtct
    19681 ggaagcccgc gcgatttgcc agggtgacaa agttatcctg aaactgtggt ttgccatcga
    19741 ctacgcgatt gagcgttaag ccaagcgatt tctgcgtgga gccactggcg gcaatgtagt
    19801 cagcaaatat caaaccgtta acgctgctgg caaacggggt attgtcccag tgaccgccaa
    19861 aggcaccgag ggcatcgcga cgcgcgcttt caccgatcac caccacatag gtctgatact
    19921 tcggcttaac ggcagtgacc gtccaggtat ctttcattcc ggaaagtttt gccatccgtt
    19981 cttgctcttc aatcacctcg ttattattga cgatgacatc cttagcaaaa cgaaataccg
    20041 gatagccagt atctttcagt ttaaatacgc caccccaggc caggttttgc acgggagcaa
    20101 caaaaaatgt cgccacactg aataccagac acaggctgtc gaaggtattc cagcgcgctt
    20161 tttcattctc tttttttcgc ctgattgcta ttacgccgag cgcaaaaata aataagccga
    20221 ccaggtagct gtaccacgga aaaatcgtca ggatttcggt cgactcttcc atattggtgg
    20281 agtgcaatgc cagcaatgta ttgaaattag gcgcgccgta ggcctgagca aaagggaaat
    20341 aacaagccgc caccagcgaa ctgacaccga ccagaacttt ttgtacgcga ggcaatgttc
    20401 gccatagcaa aagcaaaata gccgtaaacg cagcggtgta gagcaaactg aaggggtaac
    20461 ctaagccgag gttaattaat agcgactgta aaaagtagaa cgcagtccac gggctaaata
    20521 cccggctacg ggtaacaagc gattctttga gggttaaatt catatgccac tatcgtaaca
    20581 aacgccatgt gcttaccctg gcgtcaaggg tcaatacctg caagagtgcg tgaagagggg
    20641 aaaggggtcc gcatccgcga gccgcaatat acgcagggct gcaagaagat agagcgagcc
    20701 gcagttcagg tcaactactg acgaacaaat cttttgcgaa ggggattgca aatccgacaa
    20761 aaagtggggt ttttcagacc aaaacgcgtc gtgcctgcat gaaagcagga gtgaagcggc
    20821 gtaccgcgac gccgctattt atcggaaggt ttatcttgct gcggtttgtt gttgaccata
    20881 tcgcacaaca tagagagcag cattaaccgt actttaaagg gagaatgact aaacacgcgc
    20941 atacacctct tgaactcatt cataagacct cctgacttgc taatcccgtc gatccttgag
    21001 ggatgattgc attacataca gatatagcac aggctatatt atatagctat tgctaaaacg
    21061 ttaatttttt gtaccttcgc aactctggtt tacaatgtgc gcacgaaatg agaacgctat
    21121 gtatgcgtca ccataatgag gaagcacaat gagtcgtcgc gcaggtacgc caacagcaaa
    21181 aaaagtgacg cagttagtga acgaggaaga gcacgttgaa gggttccgcc aggtcagaga
    21241 ggcgcatcgg cgcgagctta ttgatgatta cgttgagctg atttctgact tgatcaggga
    21301 agtgggggaa gctcgtcagg tagacatggc tgctcgtttg ggagtttcgc aaccgacggt
    21361 ggctaaaatg cttaagcgac tggcaactat ggggctgatt gaaatgatcc cctggcgtgg
    21421 cgtgttttta acggcagaag gagagaagct ggcgcaggaa agccgcgagc gacatcagat
    21481 agtcgaaaat ttcttgttgg tgttgggcgt cagtccggaa atcgcccgtc gcgacgcgga
    21541 aggcatggag caccatgtta gtgaagagac gctggatgcc tttcgtttgt ttacccagaa
    21601 acacggtgcc aaatgagcct gcctttttta cgcacgctgc aaggcgatcg tttttttcag
    21661 ttattaattc ttgttggtat cggattaagt tttttcgtgc cctttgcacc gaaatcctgg
    21721 cctgctgcta tcgactggca caccatcatc accttaagcg gcctgatgct gctgaccaaa
    21781 ggtgtggagt taagcggtta ttttgatgtg ctggggcgca aaatggtgcg ccgctttgct
    21841 acggagcgtc ggctggcgat gtttatggtg ctggcggcgg cgctgctttc tacctttctg
    21901 accaacgatg tcacgctgtt tattgttgtt ccgctgacta tcacgctaaa aaggctgtgt
    21961 gagatcccgg ttaatcggct gattattttt gaggcgctgg cagtcaacgc tggttcgcta
    22021 ctgacgccaa ttggcaaccc gcaaaatatt cttatctggg gacgttctgg tctttcgttt
    22081 gccggattta ttgcccaaat ggcaccgctg gctggcgcaa tgatgctgac gctcctgctg
    22141 ttgtgctggt gttgtttccc tggaaaggca ctccaatacc atacgggggt gcaaacaccg
    22201 gagtggaaac cgcggctggt gtggagttgt ctggggctgt atatcgtctt tctgacggcg
    22261 ctggagttca aacaagagct gtggggactg gtgattgtgg cggcgggctt tgcgctgctg
    22321 gcgcgtcgcg tggtgttgag tgtggactgg acgctgctgc tggtgtttat ggcgatgttt
    22381 atcgacgtcc atttactgac ccagcttcca gcgttgcaag gcgtgttggg taacgtgagt
    22441 catttatctg aacccggatt atggttaacg gcaatcggtt tatcgcaggt gatcagtaac
    22501 gtgccgagta ctatattgtt gctgaactat gtgccgccgt ctttattact ggcatgggcg
    22561 gtaaacgtag gtggctttgg gttattaccc ggatcgctgg caaatttgat tgcgctacgt
    22621 atggcgaacg atcgccgcat ctggtggcgt ttccatctct attcaatacc gatgctgttg
    22681 tgggcggcgc tggtgggata tgttttgttg gttatgatcc cggcctggta aggtctggca
    22741 aataaaaaag gcggattatg agtccgcctt tgttttacgt tttgaagatt aattcagacg
    22801 aaccggcatc ccggagcggt ttttaatcgc ttcatcaaga acaacctggt caacatctgg
    22861 ctggccggtc actgtctgca cgctcttcgt cagggtaatt ggcacaattt cctgaccttc
    22921 aaattgggct tcggtggtag acagcgggtt atggacttca atgtaacggc tgccgtctgg
    22981 ctcggtggtc gcttttaccg gctcatcaat aaactgtacg cgggtaccga ccggtacttt
    23041 ctcgaacagg aatttgatgt cttcgttacg cagacgcaca caaccatgac ttacacgcag
    23101 accgataccg aagttagcgt tggtgccatg gatagcgtac aggcgaccga tgtagagtgc
    23161 atacagcccc atcgggttat ccggacctgc cggaacgaca gccggaagcg gttcgcccgc
    23221 agcgcggtac tctgcgtgca ttttggcggt cggcgtccag gtcgggcctg ctttcttacg
    23281 ctcaactttg gtggtccagt tgataggcgt atctttgcct aactgaccaa tgccgatcgg
    23341 cagcacgata acggtgttgg tccctttcgg atagtaatac agacgcatct ctgcactgtt
    23401 aatgacgatg ccttcatgaa cggtatccgg caggatcagc tgctgcggaa tgttcagtac
    23461 agtaccgcct ttcggcagga aggtatccac acccgggttc gcttccatca tattggaaag
    23521 ccccatctgg tactccgcgg caaaatactc cagcggctga gtgttacctt caggaatggt
    23581 gatcacctga ttctgaccaa ccaggcgact cccgtcggtt ggcagaggat aagttaccgc
    23641 agaggcggta ctgcaaaagc cgacaacagc gaaggccgct gcgaataatg ttttcaattt
    23701 catattcatg ttaagcgaga ttttgtgcct ggcaggccat tgggttgaga atattagagt
    23761 attggaagcg cattataagt tcattccagc tcacagtgaa atcagatgtg tacgaaatca
    23821 cattttttgc ctttggcttg agtgtagatc ttaagcgagg agcaggatct tctttcagac
    23881 ttatggcata atgcgcggtt tgtcatatct cttttcagga tacgcctgtg ttagtttcca
    23941 gtaacgtcac catgcagttc ggcagtaagc cgttgtttga aaacatttcc gtcaaatttg
    24001 gcggcggcaa ccgttacggc ctgattggcg cgaacggtag tggtaaatcc acctttatga
    24061 agatcctcgg cggcgacctt gagccgacgc tgggtaacgt ttccctcgat cccaacgagc
    24121 gcattggtaa actgcgtcag gatcagtttg cctttgaaga gttcactgtg ctggatacgg
    24181 tgatcatggg gcataaagag ttgtgggaag tgaagcagga gcgcgaccgc atctatgctt
    24241 tgccggaaat gagtgaagaa gacggctata aagtggccga tctggaagtt aaatacggcg
    24301 aaatggacgg ttactctgcg gaagctcgtg ccggtgaatt gttgcttggc gtgggaattc
    24361 cagtagaaca gcactacggc ccgatgagtg aagttgctcc tggctggaag ctgcgtgtgt
    24421 tgctggcgca ggcgctgttt gctgatccgg atattctcct gctcgacgaa ccgaccaaca
    24481 acctcgacat cgacaccatt cgctggctgg aacaggtgct gaacgagcgt gacagcacca
    24541 tgatcatcat ctcgcacgac cgtcacttcc ttaacatggt ctgtacccac atggcggatc
    24601 tggattacgg cgagctgcgc gtttatccgg gtaactacga tgagtacatg acggcggcga
    24661 cccaggcgcg tgaacgtctg ctggccgata acgccaagaa gaaagcgcag attgctgagc
    24721 tgcaatcttt cgttagccgc tttagcgcca acgcctcgaa atctcgccag gcaacttcgc
    24781 gcgcgcgcca gattgataaa atcaaactgg aagaggtgaa agcctccagc cgtcagaacc
    24841 cgttcatccg ttttgaacag gataagaagc tgttccgtaa cgcgctggaa gtggaaggtc
    24901 tgaccaaagg gtttgataac ggtccgctgt ttaaaaatct caacctgctg ctggaagtcg
    24961 gtgaaaaact ggcggtactg ggtaccaacg gcgtcggtaa atcaacgctg ttgaaaacgc
    25021 tggtgggcga tctgcaaccg gacagcggca ccgtaaaatg gtctgaaaac gcgcgcattg
    25081 gttactatgc tcaggaccac gaatatgagt ttgaaaatga tctgaccgtg ttcgaatgga
    25141 tgagccagtg gaagcaggaa ggcgatgacg agcaggcggt gcgcagtatt ctcggtcgtt
    25201 tgctgttcag ccaggacgac atcaaaaagc ctgctaaagt gctttccggt ggagaaaaag
    25261 ggcggatgct ttttggtaag ttaatgatgc agaagccgaa cattctgatc atggacgaac
    25321 cgaccaacca cctggatatg gaatccatcg agtcactgaa catggcgctg gaactgtatc
    25381 agggcacgct gatctttgtt tcacacgacc gtgagttcgt aagctccctg gcgacccgca
    25441 ttctggaaat caccccggaa cgcgtgatcg actttagcgg taattacgaa gattacctgc
    25501 gtagtaaagg gatcgagtaa gttattatcc gtcaccccga tactgtcggg gtgacggcgg
    25561 ctgatgacaa acgcaagatt gcctgatgcg ctatgcttat caggcattaa gaaagcgcac
    25621 ttgtcagcaa tctgagtaac gtcgaatcga ttttcttaat cccacatcaa ttcacttcaa
    25681 ttaactctca aacatccatg cccaacgctc gcttaccgtg aatgttgagg tcggcaagcg
    25741 taaagcgtcc ttcccagttt gtttcatagt cctcgcgcgg gaagtcgccc ggcgatgcgc
    25801 ctttttccag tgcggccttc accttgtgcg cgtaggcaag atttttctcg cacatcggtg
    25861 ccgcaggaat gtacatcacg ttgccccaac cctgttgatt ttcaacgggg gcaacggaat
    25921 ggatgacgtc gcagtgccac cagacggagt ctccggcctc gagttttgga atgctggtta
    25981 acgcctcaat taacagtgga tgccattgct ctgataccgg caacactctt ccgggcgcta
    26041 cgccgcacag ttcatcctcc ggcacatcat caagcagcgg acgtaacagt acgtacgcca
    26101 tggcttcagg aatgggcacg acgtgcagca acccctgact aggcagcata tcagagagcg
    26161 ctgtccagcc ctggaatgtc cgaaacacgg aacatttggt ggtgttgtcc accgtgtact
    26221 cttcaacttc cgtacgatgt gccgcatgcc agggatcgta tttcgccaga ttgccattaa
    26281 agacgttagc gaaaacgtgc tgatacgctg gaagcagcca gcgttccagc gccccggagt
    26341 cggtatgcgc tccaagacct ttggaggtcg ttccgggcgg acggcggcgg atacggtcag
    26401 ggtagatgac gctcacatcc gggttaaacc attgctttcc atcactttca aatgtccaca
    26461 gacgattgag aaacgactgc gcattcgcca tttcttcact ctggcgggcc tgcatttgcg
    26521 cctgcgacca gtagatgggg taaatctcgg gacgtgaagc gctgagcgtc ccgaagaaat
    26581 tatcgccggg gcctttgtag acctcgtcaa agcggttgcg gtccagatag tccagcatcg
    26641 actgatccca gcctagcgct tgttcgcggg ggaaatggcc ttttatcacc gcacaaccgc
    26701 gacgtttaat ctgttcgcgc tgctctgcag taacatgacc tgctttgata tcggcataag
    26761 acagcaccgg ccagacggca tcgccctgtg ctttgagtgc gttgatttca gccactcgcg
    26821 tggcaatgtc atcgcttagc tgattaaaga tctgctggac gtcgccaagc tgcgcccgca
    26881 gcgcgtgctt catctgacgg atagctgctt tgtgatcggc aggcaatgtg tcgctggtaa
    26941 aagtagaagc cataaccacc tcgcattaac tttcattcga aactatgctg atttgcatgt
    27001 gacaatataa gttataaata agttaatgca agtttaatga tttgatgtga tgtacaggat
    27061 gtaaaaagaa gggataaccg gagcgcatgg ctccggtaga gaggtcagct gttaaaaggg
    27121 gatgtgttat ccagtacagc ctgaatcaca ttcagcgcgc cttcatgatt attatcatcg
    27181 gtagcgtaac gggcgatttg tttaatgttt tccgcagcat tgcccatcgc aaaggaataa
    27241 cgcgccattt tcagcatctc cgcatcgtta ccgctgtcgc caatcgctac cacattttgc
    27301 ggtgacagat cccagcgttt cagtaaccgc gaaataccgt ttgctttatg tagaccggga
    27361 ataatcaggt cgataaagcc aaaaccactg gtgacgggtt tcataatgcc atcgagcgct
    27421 atgtgcagtt tgtcgatcac taacgggatt tgttcatccg gcaggttgag tgaaaacttg
    27481 aacagtacgt cgtcaatctc ctgataatct tttacaggtt tcaggcgatg gtagtgtttt
    27541 gccatcagtg cgacaaatgc ttcgggggca ttttcgctga catatgcact ttgcagaccg
    27601 caggcgacaa aattgagttg cttatctttt agcaactcgc caataacaat ccgcgattca
    27661 tgtcgggtca gttcgccgtg gaacagttgc ttgccatgtt cgtaaaccag tgcgccgttt
    27721 tccgcgacaa aagagatctc atcctttagc tcaggaaaga atgaaataag ctggtaatac
    27781 tgattaccgc tggcgacaac gaactcaatg ccgcgctttt tcagttcctg atattgcgcc
    27841 ataaaacgtg gttggttgta cgttttgacg tcgttaagaa aagtaccgtc catatctgtg
    27901 acgataactt ttacgctcat aggtgtgctc ccggctcgaa aatgaaaccg taacagtgta
    27961 ataacaatgt gacgcagagc acaaattata tttcgaatga aagtaaggat gaaattgatg
    28021 atgtgaatga tttagcccgg cgacgtctcc gccgggctga ggggattaca gcatatgttc
    28081 ggtacgggcg atgatatcgt cctgagcgtc tggagagagc gcggtgaaga aggcggaata
    28141 acccgcgaca cgcactacca gatcgcgata ctgatcggga tgttttttcg cctccagcag
    28201 cgtttcgcgg gaaacgatgt tgtactgaat atgccagcct ttatgcactt caaagaaggt
    28261 gcgcagcagg atcatcagtt tctgcttgtc agattcgttc tccagtgttg ctggattcag
    28321 tttctggttg agcaacaccc cgccgagaat cgctgccgta ggcagtttac ccaccgagcc
    28381 aatgaccgca gtagggccaa gatgatcagt accggaagcc gggcttgcgc cttctgccag
    28441 cggggtgtgg gctttacgtc cgtctggcgt tgccatagtt tgcgcgccaa acggtacgtt
    28501 agcggagata gacgacgtac ccgcgtaata gttgccgcca accggaccac gaccgtagcg
    28561 cgggttatgg tactgtttca gttcgtcgat ataggtctga taagcgcgag ccagcagcgt
    28621 atcgacagta tcatcgtcgt tgccgtactt cggcgcaccg ttaatcagcc gctgacgcag
    28681 ctgctcgtga gtcaggccgt cgaagtcatt cgccagtgcg gcagccagct gttgctgact
    28741 aatcgcgccc tgttcaaata ccagtttctt cacggccgcc aggctgttgc cgaggttggc
    28801 aatgccaacc tgcaggccag aaacccagtc atatttcgcg ccgccttgct tgatactttt
    28861 cgctcgctca atacagtcat ccaccagcgc cgagcagaga atatcgtgca cgttctcttc
    28921 cagcatggtg tcgacgacat attcgatttc gattgattta cgcgtgtagt aacggatttg
    28981 cgtatcccac gcgtccatca cttcatcgaa gttgttgaag ttacctgccg acaacgcttt
    29041 ttcttgtggc aggaacactt tgccgctggt ggcatcacgc ccgccttcca gcgccgccag
    29101 catcacgcgg gcgaagttga taaagctcat gccggtacag cgatagcccc atttgccacc
    29161 gacggcggtt tcgatacaac caatcgctgc atagttgtaa gcatcctgcg gttcaatacc
    29221 gagtttaata aattccggga tgacgatttc gtcgttgttg aacgccggca tcccgaagcc
    29281 gcaacggatc acctgtacgc aggcgtcgag gaaatcgttg ctcattcccg cgtggtaacg
    29341 cacgctgagg ttaggctgag tggaacgcag gcgaccgcag gattcgagga tcgcgtaaga
    29401 gagtggattc accgcgtcca ttggttgacc atcaaccaga ttttgtccgc caatggtgac
    29461 gttctgatac agaggacttc ccgcagaggc ttttgagtgt gagcctgagc ggattttgtt
    29521 cacttccagc agtttcagcc agcagctatg cagcaattcg atggcgtgtt cgcgatccag
    29581 tgtctggttg agttcaacgt cgcggcgata gtacgggtag agatactggt ccatacgacc
    29641 aaacgatact gagtgaccgt tagattcgat ctgcaaaatc aactggatga agtaacacag
    29701 ttgcagcgcc tgccagaaag tctgcggcgg ctggtgggcg ataagatcgc agttttctgc
    29761 catcgccagc agttcatcgc gacggctttc gcgggtttcg gtcgcggcca ttgcacgcgc
    29821 cagggcagcg aaacgttcaa tgtgttcact gactgccacc agcacgatat caatcgcttt
    29881 caggaactgt tcgccgtgca gatcttccag taccgtcaga ttgatgcgcg agcgacgttc
    29941 cgctactttc tcgcgcagac catcaagcct ttttccagca gcagcgggaa attcaccgcc
    30001 aggtgcgcat cgccggaggt catattgcct tccgctttaa tgattccggt cgccagcaga
    30061 cctttttgct catcggtaaa catgccgtag cagcgatcct gtaccgtctg accgcgccac
    30121 cacgggcaca cttcatgcag aacgcgtttg ttctcttcgc tcaccgcaaa gccagcaccg
    30181 ggacgatctg ccagatcatc aatctctttt tcgatccacg agacagtata ttccgggaag
    30241 atcggcgcgg cgcgaacttc gcttgcctgg ttgccgatga tcaactcatc gtgcttgatc
    30301 cagatagtgc gattcgccag gtgatgtgcc agcgccagcg cgcgacgtac agggatcggc
    30361 ttatcgagat gttgttgata catctcggta tagtgctgcg cgcgctcggt acaaaccggc
    30421 ggtttcacaa tatgcaccag cgcatttttg tgcgctttaa tgcggtcgct gagcgtgtcc
    30481 agtttcagtg tggtcatggt tgttatcctc gtaaggtcgc ggttaaccct ttctggcagg
    30541 catactgctg ggcaaagtcg agcagttctg gcgcatcaag cggtttttcc ggggcgtcat
    30601 agggcagatt aagtaagtga tatttgttga tgcccagcgt gtggtagggc aggaaatgaa
    30661 tttcgccaac gtgcagctcg tcggcggcaa aatcggtaat ggctttcaca gaggtttcgt
    30721 cggcattaaa gccttgaatc agcggcacgc ggataatgat ttttttgccc gctgcggcga
    30781 gttttttcag gttatccagc accctggcgg cgttaccgtc ggtccactgt ttaaacggcg
    30841 cgtcggcaac gtgttttaaa tcggcaagaa acagatcgat atagggcaga gaaggggcga
    30901 tatatttcca cggcacatgc aggcaggttt ctaccgcagt atgaatgccg gcctcgtggc
    30961 tggcttgcag tagcgccgtc gccatttccg gctgcatgaa tggttcaccg ccagaaagtg
    31021 ttaagccgcc gccgctgcga tcgtaaaacg gtttatcgcg cagaacggtc accatgatct
    31081 cctcaacgct tttcacttcc ccacacacgg ttaacgactg tgtcgggcag cagtcggtta
    31141 acgccgtcag atgctccggg gttaactttt cccgatgaat aagcaaacca ttcagcgcgc
    31201 gctcaatcac ttccggcgcg gtcttagcgc acagctcaca gccttccagg cacagacgtg
    31261 ggtcatacag cagatcctgc gtgcgggcgc ggctttccgg gttctgacac cagcggcagc
    31321 ccagcgaaca gcctttaaga aatacgaccg tgcggatacc ggggccatca tgggtcgagt
    31381 agcgctgaat attgaaaatc atagttgcct ctctgtttcg ttcaaatatt aaaatacttt
    31441 cgaatgaaag ttagattgat gtgcgtcaac tgttcagaga gttttcccgt gatagtctac
    31501 attcagacaa aaagtacatt ttgaggatgg ttatggaact gtatctggat acttcagacg
    31561 ttgttgcggt gaaggcgctg tcacgtattt ttccgctggc gggtgtgacc actaacccaa
    31621 gcattatcgc cgcgggtaaa aaaccgctgg aggttgtgct tccggaactt catgaagcga
    31681 tgggcggtca ggggcgtctg tttgcccagg taatggctac cactgctgaa gggatggtga
    31741 atgacgcacg taaattgcgt tctattattg cggatatcgt ggtgaaagtt ccggtgacca
    31801 ccgaggggct ggcagctatt aagatgttaa aagcggaagg gattccgacg ctgggaaccg
    31861 cggtatatgg cgcagcacaa gggctgctgt cggcgctggc aggtgcggaa tatgttgcgc
    31921 cttacgttaa tcgtattgat gctcagggcg gtagcggtat tcagactgtg accgacttac
    31981 accagttatt gaaaatgcat gcgccgcggg cgaaagtgtt agccgcgagt ttcaaaaccc
    32041 cgcgtcaggc gctggactgc ttactggcag gatgtgaatc aattactctg ccactggatg
    32101 tggcacaaca gatgattagc tatccggcgg ttgatgccgc tgtggcgaag tttgagcagg
    32161 actggcaggg agcgtttggc agaacgtcga tttaaccgga cgttctgcat cctcataaat
    32221 tgctgatgac gtggcggagt gcaccgatgc ctgccgcgct tggaaatctg tgcccgaacc
    32281 gtaggccaga taaggcgttc acgccgcatc tggcgtctgg agcaaatgcc tgatgcgacg
    32341 cttgtcgcgt cttatcaggc ctggaggtgg caattactgc ccgcacacct cacaccccgg
    32401 attgcgcatc agtttcattt cgcgaaactg acaggtcatc gcatcgtaca tgacgatttt
    32461 cccgctggcc ggtttgccat aacctgccag cagtttgatc gcttccattg cctgcaacga
    32521 accaatcacg ccgatcaacg gtgccatcac gcctgcttct acgcaggtta acgcgttctc
    32581 gctaaacaaa cggctaaggc agcgatagca cggttcgccg tcctgataag tgaagaccgt
    32641 gatttgccct tccatacgaa ttgccgcgcc ggaaaccagc ggcaccttcg cggcaaaaca
    32701 gcctgcgttc agttgattac gtaccgcaac gttatccgta cagtcgagca caagattgtg
    32761 ttcagcaatc aatgctgcaa gttctgcgtc atccagcagt gcattgactg gcgtaatcgc
    32821 gatatgtggg ttgatccgcg tcagagcgtc acgggcggat tccaccttcg gttgcccgac
    32881 cgtagcatca ctgtgcagcg tctggcgttg cagattcgag agcgaaaccg agtcgaagtc
    32941 gagcagcgtc aggttgccga caccggcgct tgccagatac tgcgaagctg cgcagccgag
    33001 tccaccgagg ccgacgacca gcacacgaga atctttcagc gcctcctgac cgtcaaaatc
    33061 aaaaccgcgc agaatgattt gccggttgta gcgcagcatc tcttgatcgc tgagttccgc
    33121 cattacaggc ctccgaacaa cgcgttaaac ggttctactt ccacccattc gcccacttcc
    33181 acattgccgc gatcgcgttc cagcacgata aagcagttgc cgaggctaaa ggagctgaat
    33241 atatgtgaac cctgatgtcc ggtggtcgtc acttccagtt cgccatcggc gttgcggtgc
    33301 agcacgccgc gctggaaatc aagacgtcct ggcgtttttt tcaggcggga cgctgtgcgt
    33361 acgcgctggc gcgcgggcag gccgctggcg gtgttgccgc ttagttttgc cagcaaaggc
    33421 tgtaccagtt gatagaaggt cagcgtcgct gaaaccgggt tgcccggcag gccgcagaac
    33481 cagctattgc tgagtttacc gaacgcgaac ggtttacctg gtttaatcgc cagcttccag
    33541 aaggcgatct cccccagctc ttcaagaatc gttttggtgt aatccgcttc acccactgaa
    33601 acaccgccgg aactgatcac cacatccgcc tggctgtcgg cttcaataaa tgcggcgcgc
    33661 agggcatgtg gatcgtcgcg gataatccct aagttgatta cctcgcaacc cagctgttct
    33721 aacatcaggt gtacggcgag acggttggta tcgtagattt ggccgtcgcc cagcggctgg
    33781 cctggcaact ggagttcatc accggtagaa aaaagcgcta cacgcacttt acgaatcacc
    33841 ggaacttcgg caatccccag tgaagcaatt actggcagct ctgcggtagt caggcgagtc
    33901 cccgccggga aaacaaccgc acctgcagag atatcttcac cgcgacggcg aatattttgc
    33961 ccgctacgca cttcagcagt aaaacgcacg ccattgtccg tttgttcagt ctgctcctgc
    34021 atcaccaccg cttcgcagcc ttccggcacc ggcgcaccgg tcataatacg aatgcaggta
    34081 cccgcaggcc attcaccatg gtacggctga ccggcaaagg atttaccggc aacgggcagc
    34141 ggttgtccgg aggtgatatc ggccaaacgt accgcgtaac catccattgc ggagttatca
    34201 aaccctggga catccagcgg cgaaacgaca tcgctcgcca gaatacgacc aaaacactga
    34261 accagtggca gcgtttcctg ggcggtcagt ggggtgacgc gagaaagcat ctcattaagc
    34321 gcggtgtcga gcgacatcaa tccggtggta aattccatga aaacactcct gcggaggcaa
    34381 aatcgaattt gcctattatg tcagaaaaac gccacagact gtatgccacc tcgggcgtag
    34441 cgctgggtcc tgcctttaca tgccatatcc atctttctat attcaaaaat tgaatgagta
    34501 attcataaaa attctgatat ttatagcaaa agtggcgagc cacccttaat ggacgaatac
    34561 tatgggcaaa gcagtcattg caattcatgg tggcgcaggt gcaattagcc gcgcgcagat
    34621 gagtctgcaa caggaattac gctacatcga ggcgttgtct gccattgttg aaaccgggca
    34681 gaaaatgctg gaagcgggcg aaagtgcgct ggatgtggtg acggaagcgg tgcgtctgct
    34741 ggaagagtgt ccactgttta acgccggaat tggcgccgtc tttacgcgtg atgaaacgca
    34801 tgaactggac gcctgtgtga tggatggtaa caccctgaaa gccggtgcgg tggcgggcgt
    34861 tagtcatctg cgtaatccgg ttcttgccgc ccggctggtg atggagtaaa gcccgcatgt
    34921 gatgatgatt ggcgaagggg cagaaaattt tgcgtttgct catggcatgg agcgcgtctc
    34981 accggagatt ttctccacgc ctttgcgtta tgaacaacta atggcagcgc gcgaggaagg
    35041 ggcaacagtc ctcgaccata gcggtgcgcc actggatgaa aaacagaaaa tgggcaccgt
    35101 gggggccgtg gcgttggatt tagacggcaa tctggcggca gccacgtcca cgggcggaat
    35161 gaccaataaa ttacccggac gagttggcga tagcccctta gtgggtgccg gatgctacgc
    35221 caataacgcc agtgtggcgg tttcttgtac cggcacgggc gaagtcttca tccgcgcgct
    35281 ggcggcatat gacatcgccg cgttaatgga ttacggcgga ttaagtctcg cggaagcctg
    35341 cgagcgggta gtaatggaaa aactccctgc gcttggcggt agcggtggct taatcgctat
    35401 cgaccatgaa gggaatgtcg cgctaccgtt taacaccgaa ggaatgtatc gcgcctgggg
    35461 ctacgcaggc gatacgccaa ccaccggtat ctaccgtgaa aaaggggaca ccgttgccac
    35521 acagtgatga acttgatgcc ggtaatgtgc tggcggttga aaatcttaat attgccttta
    35581 tgcaggacca gcagaaaata gctgcggtcc gcaatctctc ttttagtctg caacgcggtg
    35641 agacgctggc aattgttggc gaatccggct ccggtaagtc agtgactgcg ctggcattga
    35701 tgcgtctgtt ggaacaggcg ggcggtttag tgcagtgcga taaaatgctg ttgcggcggc
    35761 gcagtcgtga tgtgattgaa cttagcgagc agagcgctgc acaaatgcgc catgtgcgcg
    35821 gtgcggatat ggcgatgata tttcaggagc cgatgacatc gctgaaccca gtatttactg
    35881 tgggtgaaca gattgccgaa tcaattcgtc tgcatcagaa cgccagtcgt gaagaagcga
    35941 tggtcgaggc gaagcggatg ctggatcagg tacgcattcc ggaggcacaa accattcttt
    36001 cacgttatcc gcatcaactc tctggcggga tgcgccagcg agtgatgatt gcgatggcgc
    36061 tgtcatgccg cccggcagtg ctgatagccg atgagccaac caccgcgctg gatgtcacta
    36121 ttcaggcgca gatcctgcaa ttaatcaaag tattgcaaaa agagatgtcg atgggcgtta
    36181 tctttatcac tcacgatatg ggcgtggtgg cagagattgc cgatcgggta ctggtgatgt
    36241 atcagggcga ggcggtggaa acgggtaccg tcgaacagat ttttcatgca ccgcaacatc
    36301 cctacacccg tgcgctgtta gctgctgttc cgcaacttgg tgcgatgaaa gggttagatt
    36361 atccccgacg tttcccattg atatcgcttg aacatccagc gaaacaggcc ccccccatcg
    36421 agcagaaaac ggtggtggat ggcgaacctg ttttacgggt gcgtaatctg gtcacccgtt
    36481 ttcctttgcg cagcggtttg ttgaatcgcg taacgcggga agtgcatgcc gttgagaaag
    36541 tcagttttga tctctggcct ggcgaaacgc tatcgctggt gggcgagtct ggcagcggta
    36601 aatccactac cgggcgggcg ttgctgcgcc tggtcgaatc gcagggcggc gaaattatct
    36661 ttaacggtca gcgaatcgat accttgtcac ccggcaaact tcaggcattg cgccgcgata
    36721 ttcagtttat ttttcaggac ccttacgctt cgctggaccc acgtcagacc atcggtgatt
    36781 cgattatcga accgctgcgc gtacacggtt tattgccagg taaagaagcg gttgcacgcg
    36841 ttgcgtggtt gctggagcgc gtgggcctgt tacctgaaca tgcctggcgt tacccgcatg
    36901 agttttccgg cggtcagcgc cagcgcatct gcattgctcg cgcgttggca ttgaatccaa
    36961 aagtgatcat tgccgacgaa gccgtttcgg cgctggatgt ttcaattcgc gggcagatta
    37021 tcaacttgtt gctcgatctc cagcgtgatt tcggcattgc gtatctgttt atctcccacg
    37081 atatggctgt ggtagagcgg attagtcatc gtgtggcggt gatgtatctc gggcaaattg
    37141 ttgaaattgg tccacggcgc gcggtcttcg aaaacccgca gcatccttat acgcgtaaat
    37201 tactggcggc agttccggtc gctgaaccgt cccgacaacg accgcagcgt gtactgctgt
    37261 cggacgatct tcccagcaat attcatctgc gtggcgaaga ggtggcagcc gtctcgttgc
    37321 aatgcgtcgg gccggggcat tacgtcgcac aaccacaatc agaatacgca ttcatgcgta
    37381 gataacattc aggcggagaa taaaatggca agagctgtac accgtagtgg gttagtggcg
    37441 ctgggcattg cgacagcgtt gatggcatct tgtgcattcg ctgccaaaga tgtggtggtg
    37501 gcggtaggat cgaatttcac cacgctcgat ccgtatgacg cgaatgacac gttatctcag
    37561 gccgtagcga aatcgtttta ccaggggctg ttcggtctgg ataaagagat gaaactgaaa
    37621 aacgtgctgg cggagagtta taccgtttcc gatgacggca ttacttacac cgtgaaattg
    37681 cgggaaggaa ttaaattcca ggatggcacc gatttcaacg cagcggcggt aaaagcgaat
    37741 ctggaccggg ccagcgatcc ggcgaatcat cttaaacgct ataacctgta taagaatatt
    37801 gctaaaacgg aagcgatcga tccgacaacg gtaaagatta ccctcaaaca gccgttctca
    37861 gcgtttatta atattcttgc ccatccggcg accgcgatga tttcaccggc agcgctggaa
    37921 aaatatggca aggagattgg ttttcatccg gtgggaaccg gaccgtatga actggatacc
    37981 tggaatcaga ccgattttgt gaaggtgaaa aaattcgcgg gttactggca gccaggattg
    38041 cccaaactgg acagcataac ctggcgtccg gtggcggata acaacacccg cgcggcaatg
    38101 ctgcaaaccg gtgaagcgca gtttgctttc cccattcctt acgagcaggc cgcactgctg
    38161 gagaaaaaca aaaatatcga gttgatggcc agtccgtcaa ttatgcagcg ttatatcagt
    38221 atgaacgtga cgcaaaagcc attcgataac ccgaaggtcc gtgaggcgct gaattacgcc
    38281 attaaccgtc cggcgctggt gaaagtggcc ttcgcgggct atgcaacgcc agctactggt
    38341 gtggtaccgc cgagtatcgc ctacgcgcaa agttataaac cgtggcctta cgatccagtg
    38401 aaagcgcgcg aattactgaa agaggcggga tatcccaacg gtttcagtac cacgctgtgg
    38461 tcgtcacata accacagcac cgcgcagaaa gtgctgcaat ttacccagca gcagttagcg
    38521 caggtcggga ttaaagccca ggtgactgcg atggatgccg gacagcgggc ggcagaagtt
    38581 gaaggtaaag ggcaaaaaga gagcggcgtg cggatgttct acactggctg gtcggcttca
    38641 accggcgaag cggactgggc actatcgccg ttgtttgcct cgcaaaactg gccaccgacg
    38701 ctgtttaata ccgcgtttta cagcaataaa caggtggatg acttcctggc tcaggcactg
    38761 aaaactaatg atccggcgga aaagacccgc ttatataagg cggcgcagga tatcatctgg
    38821 caagagtcgc cgtggatccc gctggtggta gaaaaactgg tgtcggcaca cagtaaaaac
    38881 ctgaccggtt tttggatcat gccagatacc ggcttcagct ttgaagacgc ggatttgcaa
    38941 taagcaacgc agggagtgga atgcttaatt acgttatcaa acgattactg gggttgattc
    39001 cgacgctgtt tatcgtctcg gtgctggtgt ttttatttgt gcatatgctg cccggcgatc
    39061 cggcgcgatt gattgccggg cccgaagctg atgcgcaggt tatagaactg gtgcgtcagc
    39121 agctggggtt ggatcagccg ctgtatcacc agttctggca ctatatcagc aatgctgtgc
    39181 agggggattt tggcctgtcg atggtgtcac gtcgtccggt tgccgatgag attgccaggc
    39241 gctttatgcc aacgctgtgg ctgaccataa ccagtatggt ctgggcggtg atatttggta
    39301 tggcggcggg aattatcgcc gccgtctggc gtaaccgttg gccggatcga ttgagtatga
    39361 ccattgcggt gtcggggatc tcgtttccgg catttgcgct ggggatgctt ttaattcagg
    39421 tattctccgt tgaactgggc tggctgccta ccgtgggagc agacagttgg cagcactaca
    39481 ttttaccctc cctgacgctc ggcgcggcag tggccgccgt gatggcgcgc tttacccgcg
    39541 cgtcgtttgt cgacgtttta agcgaagatt atatgcgtac cgcgagggcg aaaggggtga
    39601 gcgaaacctg ggttgtcctc aaacacgggc tacgcaacgc gatgatcccg gtagtgacca
    39661 tgatgggctt acagtttggc tttttgctcg gtggttccat cgtggtggag aaagttttca
    39721 actggcccgg acttggacgc ttactcgttg actccgtaga aatgcgcgat tacccggtga
    39781 ttcaggcgga aattctgctt ttctcgctgg aatttattct tatcaactta gtggtggatg
    39841 tgctttacgc cgccattaac ccggctatca ggtacaagta aggatgcgac tatttaactg
    39901 gcgacgtcag gcggtgttaa acgccatgcc actggtcaaa cctgaccagg tacgtacacc
    39961 gtggcatgaa ttctggcgac gatttcgccg tcagcatatg gcgatgaccg ccgcattatt
    40021 cgttatttta ttgattgtgg tggccatttt tgcacgctgg atcgctccct atgacgccga
    40081 aaattatttt gattatgaca atctgaataa cggaccttct ttgcagcact ggtttggcgt
    40141 cgattcactg gggcgcgaca ttttcagccg tgtcctggtt ggtgcgcaaa tctcgctggc
    40201 ggcgggcgtg tttgccgtgt ttatcggtgc ggcgataggg acgttgctgg gcttgttggc
    40261 tggatattat gaaggctggt gggatcggct gatcatgcgc atttgcgatg tgctgtttgc
    40321 cttcccgggt attttactgg cgatcgctgt tgttgcggtg ttgggaagcg gcattgctaa
    40381 cgtgattatt gcagtcgcca ttttttccat ccctgcgttt gcccgcctgg tgcgcggcaa
    40441 cacgctggtg ttgaaacagc aaacctttat tgagtcagca cgcagtattg gtgccagcga
    40501 tatgaccatt ttgttgcgtc atatcctgcc tgggaccgtc tcttctatcg tggtgttttt
    40561 caccatgcgc attggtacct cgattatctc tgccgccagc ctgtcatttc tcggcctcgg
    40621 tgcgcagccg ccgacaccag agtggggagc aatgctcaat gaggctcgag cggatatggt
    40681 tatcgcgccg catgtcgctg tttttccggc cctggctatt tttctgactg tactggcgtt
    40741 caatttgttg ggcgatggtt tacgcgatgc gctggatccg aaaattaaag gatagttacg
    40801 tttgaatatt gcttgaaaga gtaatcacct cacaggaaat tattgcccta agcaagtgtt
    40861 gtaactttct gctgattttg tagaatcggg taatttggtt aaatagccgc agcaagggac
    40921 tgtttttgca gcggcacagc gttcagacag ttattttgtt aaatgtatta acatgctgag
    40981 tttatacgaa aagataaaga taaggctgat aattttattt ttattggcag cactgtcatt
    41041 tattggcctt tttttcatca ttaactatca actggtatcg gaacgcgcgg taaaacgtgc
    41101 cgatagccgc tttgaactta ttcagaaaaa cgttggctat ttctttaaag atattgaacg
    41161 ttcggccctg acattaaagg actcactgta tttattaaaa aatacagagg agattcaacg
    41221 cgctgtgatc ctgaaaatgg aaatgatgcc atttttagac tcggtgggac tggtacttga
    41281 tgataataaa tattatctct tttcgcggag ggcgaacgat aaaatcgttg tttatcatca
    41341 ggaacaagta aatggaccgc ttgtcgacga gtcagggcag gttatttttg ccgattttaa
    41401 cccatcgaaa cgaccgtggt cggtggcttc agatgactct aacaacagct ggaatccggc
    41461 ctacaattgc tttgatcgtc cgggtaaaaa atgtatctct tttacgctac gcatcaacgg
    41521 caaagatcac gatttgttag cggtggataa aattcatgtc gatttaaact ggcgatatct
    41581 gaacgagtat cttgatcaaa tcagcgctaa tgatgaagtt ctatttttga aacaaggcca
    41641 tgagatcatt gccaagaatc aactcgctcg tgaaaaactg attatttata atagcgaagg
    41701 tgattataat attattgatt ctgtcgatac tgaatatatc gaaaaaacat cagcggtgcc
    41761 aaataacgca ttattcgaaa tctattttta ttatccgggc ggtaatttat tgaacgcatc
    41821 agataaactt ttttatctgc cgtttgcgtt cattattatc gtattgctgg tggtttattt
    41881 aatgaccact cgtgtgttcc gtcggcaatt ttctgaaatg acagagctga ttaatacgct
    41941 ggcgtttttg cctgactcaa cggatcagat cgaggctctg aaaattcgcg aaggcgatgc
    42001 gaaagagatt atcagcatca aaaattcgat cgcggaaatg aaagatgccg aaattgaacg
    42061 gtcaaataaa ttgctctcac tgatctctta cgatcaggaa agtggtttta ttaaaaatat
    42121 ggcgattatt gagtccaaca ataatcagta tctggctgtg gggatcatca aactgtgtgg
    42181 tctggaagcc gtggaagctg tgtttggtgt tgatgaacgc aataaaatcg tcaggaaatt
    42241 gtgtcagcga attgctgaga aatatgcaca atgctgcgat atcgtgacat tcaatgccga
    42301 tctctattta cttctgtgtc gggaaaatgt acagacattt acccgtaaaa tagcgatggt
    42361 aaacgatttt gacagcagct ttggctaccg caatctgcgc atccataagt ctgccatttg
    42421 tgaacctttg cagggggaaa acgcctggag ttacgcagaa aaactgaaac tggcgatttc
    42481 cagtatccgt gaccatatgt tctccgagtt tattttctgt gatgacgcga aactcaacga
    42541 aatagaagag aatatctgga ttgcgcgtaa tattcgccat gcaatggaaa ttggcgaact
    42601 attcctcgtc tatcaaccga tcgttgatat taacacccgc gccattctgg gcgcggaggc
    42661 gttgtgccgt tgggtgtctg cggagcgggg gatcatttca ccgctgaagt tcattaccat
    42721 tgctgaagat atcgggttta tcaatgagct gggttatcag attattaaaa cggcaatggg
    42781 tgaattcaga cattttagtc agcgtgcatc gctgaaggat gatttcttac tgcatattaa
    42841 tgtttcgccc tggcagttaa acgaaccgca ctttcatgag cgttttacca ccatcatgaa
    42901 agaaaatggc ctgaaggcga acagcctctg tgttgagatc actgaaaccg tgatcgagcg
    42961 aattaatgaa catttttatc tcaatattga acaactgcgt aaacaagggt tacggatatc
    43021 gattgatgac tttggcaccg gtttgtcaaa cctgaaacgt ttttatgaaa ttaatccaga
    43081 cagcataaag gtggactcgc aatttaccgg cgatattttc ggtactgcgg gaaaaattgt
    43141 gcgcattatt ttcgacctgg cacgctataa ccggatcccg gtgattgcgg aaggcgtaga
    43201 gagcgaagac gttgcgcgcg aattaatcaa attaggatgt gttcaggctc aggggtatct
    43261 gtatcagaaa cccatgccat tctccgcctg ggataaaagt ggaaaattag taaaagagta
    43321 gtttacgtat gtccagaatc aataagttcg tacttacagt cagtctgctg atttttatca
    43381 tgatttcagc agttgcctgc gggatctaca ctcaaatggt aaaggaacgg gtgtatagcc
    43441 tgaaacagtc cgttattgat actgcttttg cggtggcaaa tattgctgaa tatcggcgta
    43501 gcgtggcaat tgatcttatc aacacgctaa atcccacgga ggaacagctg ttggttggct
    43561 tacgcacagc ttacgccgac tcggtttcac cctcttattt gtacgatgtc ggtccttatc
    43621 tgatttccag tgacgaatgt attcaggtaa aggagttcga gaaaaattat tgtgcagata
    43681 ttatgcaggt tgtgaagtat cgacatgtca aaaatacagg gtttatctct tttgacggta
    43741 aaaccttcgt ctattacctc tatccggtaa ctcacaatcg tagtctgata tttttgcttg
    43801 gtctggagcg tttttcttta ctgtcaaaat cgctggcgat ggacagcgag aacctgatgt
    43861 tctctctgtt taagaacggt aaaccggtga ccggtgatga atataatgct aaaaacgcca
    43921 tcttcaccgt ttcggaagcg atggagcact tcgcctattt gccgaccgga ttgtacgtat
    43981 ttgcgtataa aaaagatgtt tatttgcggg tctgtacatt gattattttc tttgccgcat
    44041 tggtggcagt gatatcgggt gccagttgcc tctatctggt acgcagagtg attaatcgtg
    44101 gtattgtgga gaaagaagcc attattaata accattttga acgcgtactg gatggcgggc
    44161 ttttcttttc ggctgccgat gtcaaaaaac tctacagtat gtataactcg gcgttcctgg
    44221 acgacctgac caaagcaatg ggtagaaaat cctttgacga agatttaaaa gcgctgccgg
    44281 aaaaaggcgg ttatttgtgc ctgtttgacg tcgataaatt caaaaatatt aacgacacct
    44341 tcggtcattt gctgggcgat gaagtgttga tgaaagtggt gaaaatcctt aaatcacaga
    44401 tcccggtaga taaaggtaaa gtctaccgct tcggcggtga cgaatttgcg gtgatttata
    44461 ctggcggcac gctggaagag ttactatcga ttctaaaaga aatcgttcat ttccaggtgg
    44521 gaagcattaa tttaagcacc agtatcggtg tagcgcattc aaatgaatgt actaccgtcg
    44581 aacgcttgaa aatgctggcg gatgagcggc tgtataagag taagaaaaac ggcagggctc
    44641 agattagctg gcagtagaat tagaatagtg ttagtacccc tgaatcaagg ggtactaatg
    44701 aatgtggtaa atcatttgca aacattttac tttgcaaaaa cttccggccc ttttattgcc
    44761 aatgtattca aaatacgtag tgccgggccg ctcggtttta tttcattacg ttcccatttc
    44821 gagacacttt cttttgacat tcccattgta tgtgccagca cggattgtga catgccgtat
    44881 cgggtgcgga tggctttgat ctcttcaccc gtcattgatt tgactttggc tatacgggcg
    44941 ctgacttcac gggctttgac catgacggca atggcatcaa cctcactatc agacatcagg
    45001 cccagtgcat tcagttcctt tgcatcttcc agcatcattt gtaatttatc gctcattttt
    45061 cacctcaatc aagaagccag tttcgatcaa atcctctatt tgcttattgc taaaggccag
    45121 aaatgcattt gccatttttt tgtaggctgc cagttcatca tcttcgattt ctttgcttcc
    45181 tttactgctt agaccactct tggaccaacc atcataaaaa aacacatgat ttgctgattt
    45241 aaaaaagata attgttctgg ctccaccact ctttccttgt tggaggggta accgtttttt
    45301 gatgacaccg ctacctaaat cagcctccca tatgccttgc atcacttctt cagcggcagc
    45361 catgagaact ttatcgtgaa taccaagttt tttcgtgctt tggctgaatc gccttgcctt
    45421 atacacaccc atacatccat ccttgtggta atgtttacta tagtaactat tacgcttatt
    45481 tcaagtaatt gcgatcaaaa ctttaatttg gtgtgaaaaa gtgagagttg cctcgagtac
    45541 gattcatttt tctcgattta tgcgtcaggg gattttgtgc gtaagggtag gacatcggga
    45601 agggtagtag aggcagatcg aagaacaata tccttgttag atctgccttt agattaatta
    45661 aacccggcta ccccacaaat cgtactcatc ggcgtgctcg actttcacac gcaggatatc
    45721 acccggctta acgttggttt caccattgag ataaaccgca ccgtcgattt ccggtgcatc
    45781 tgccatgctg cgaccaatcg cgccttcttc gtccacttcg tcgataatca ccagaatttc
    45841 acggcccact ttctcttgca ggcgctcggc ggaaatctgc tgctgcaact gcatgaaacg
    45901 gttccagcgt tcttctttca cttcttccgg aacctggtca ggcagggcat tggcgtctgc
    45961 accttcaacc gggctgtatt taaagcagcc aacgcgatcc agacgcgctt ctttcaggaa
    46021 gtcgagtagc atctggaaat cttcttccgt ctcgccaggg aagccgacaa taaaggttga
    46081 gcgtagggtc agttccgggc agatttcgcg ccactgtttg atgcgcgcca gttggcgatc
    46141 tacagaaccc ggacgcttca tcagtttgag aatgcgcggg ctggcgtgct gcaacggaat
    46201 gtccagatac ggcaggattt tgccttctgc catcagtggg atgacgtcgt ccacatgcgg
    46261 ataagggtaa acgtagtgca gacgtgtcca gatccccagt ttcgataact gttcgcacag
    46321 gctgaccatg ctggttttta ccggctcgcc gttgtggaag ccagtacgat gtttaacatc
    46381 aacgccatag gcggaagtat cctgcgagat caccagaatc tctttaacgc ccgcatctac
    46441 cagacgtttc gcttcactta atacttcgcc aatcggacgg ctcaccaggt cgccgcgcat
    46501 agacggaata atgcagaagg tgcagcggtg attacagcct tcagaaattt tcagataggc
    46561 ataatgacgc ggcgtcagtt tgacaccttg ttctggcacc aggctcagga atgggttgtg
    46621 tttcggtttt ggcacgtagt gatgaacgtg ctccagaacc tgctcatagc tatgaggccc
    46681 ggtgatttcc agcactttcg ggtggacttc gcggatctga tcttcttttg cccccagaca
    46741 accggtcaca attaccttgc cgttttcatt caacgcttca ccaatggctt ccagtgattc
    46801 ttgtaccgcg ctgtcaataa agccgcaggt gttgacgatc accatgtccg catcgtcata
    46861 gctcggtacc acgtcataac cttcagtgcg gagttcggtg agaatacgct ctgaatcgac
    46921 aaggtttttc ggacagccaa gggaaacaaa gccgattttc ggctggggag ttactttgct
    46981 catagcttaa aaaatattca gttacaggaa aggtcagggc agggattcta cagagttctg
    47041 gataaaattt gtatcgcaat ctcattcgct ggcggaggcg aaggaaatgt aaattttgtt
    47101 aattcggcgt gaagaattga tcctggacag cattatgctc aaaaaatagc catactattt
    47161 aattgcaaca aggctggaaa gaggaggatc gaagtatgtt cgttgacaga cagcgaatcg
    47221 atctgctgaa ccggttgatc gacgcacgcg ttgacctcgc cgcatacgtg caactgagga
    47281 aggcaaaagg atacatgtcc gtcagcgaaa gcaatcatct acgagataac ttttttaaac
    47341 tgaatcgcga actgcacgat aaatcgctgc ggttgaatct tcatctggat caggaagagt
    47401 ggagtgctct tcatcatgct gaagaagcat tagcgacagc cgcagtatgt ttgatgagtg
    47461 ggcaccatga ttgcccgact gttattaccg tcaacgccga taagcttgaa aattgtctga
    47521 tgagcttaac gctgagtatc cagagcctgc agaagcacgc catgcttgag aaggcctgaa
    47581 aactaagggg gagaaagcgt ctcccccttc atgtttaagt ttttgtaaaa atgaatttgt
    47641 tatctcctcc actgactacg ctttaagcca gagtcaatcc ggaggcgtta tgcatcgaca
    47701 attctttttc cttgtgcccc ttatttgtct ttcttccgct ctctgggcgg ctcctgcaac
    47761 ggtaaatgtc gaagtactgc aagacaaact cgaccatccc tgggcactgg cctttttacc
    47821 cgataaccac ggtatgttaa tcactctgcg cggcggcgag ttgcgtcact ggcaagcagg
    47881 aaaaggatta tctgcgccgc tttccggagt tccggacgtt tgggcgcacg ggcagggcgg
    47941 cctgctggac gtggttttag cgcctgattt tgctcagtct cgccgcatct ggttaagtta
    48001 ttccgaagtt ggcgatgatg gcaaagccgg aactgctgtg gggtatggcc gcttaagtga
    48061 tgatctctca aaagtgaccg actttcgcac cgtctttcgc cagatgccaa aactgtctac
    48121 cggcaaccat tttggcgggc ggctggtatt cgacggtaaa ggttatcttt ttattgctct
    48181 gggcgaaaac aatcagcgcc cgacggcgca ggatctggat aaattacagg gcaaactggt
    48241 gcgtctgacc gaccagggcg aaatcccgga tgataatcct tttataaagg aatccggtgc
    48301 gcgcgccgag atctggtctt atggcattcg taatccgcaa ggaatggcga tgaatccgtg
    48361 gagtaatgca ctgtggctga atgaacatgg cccgcgcggt ggtgatgaaa ttaatatccc
    48421 gcaaaaaggc aaaaactacg gctggccgct ggcaacctgg ggaatcaact attcaggctt
    48481 taagataccg gaagcgaaag gggagatcgt cgccgggacc gagcaacctg ttttttactg
    48541 gaaagattcg cccgccgtga gcggcatggc cttctataac agcgataaat tcccccagtg
    48601 gcagcaaaaa ttatttattg gcgcgctgaa agataaagat gtcattgtga tgagcgtcaa
    48661 cggcgacaaa gtaacagaag atggccgtat tttaacggac agagggcagc gaattcgtga
    48721 tgttcgcact ggacccgacg gttatttata cgttctcacc gacgagtcca gtggggaatt
    48781 acttaaagtt agcccacgca attagctaac gggaatcatc accactttgc gcactgcggg
    48841 gcgttcagtg agttgctgat accagcgttg cagatttgga cgcggtgtcc aggtcaggcc
    48901 aacattaaac aaattgtaga tgaacggtgc gatagcgata tcgcccacac caaactcgtc
    48961 gccagagaac cattttactt ttgccagttc cgcatcgagc agggcaaaca gggcgtcgca
    49021 ctctttgcaa ctggcatcaa tggcggcctg atcgcgctct tccggtggtg ttctgactaa
    49081 tcccatcagg atcccgcgat gagcattact gagtgtctgg tttgcccagt ccatccattt
    49141 ttctgcttcc gcacgacgtg ccggtgagtc gatccacagg cgtttttgcc cgtactgtgc
    49201 cgccagatag cggacaatgg cgtttgattc ccaaagaatg agatcacttt cgtcgtcacg
    49261 caacaacggc accagcccgt tagggttcat cgccagaaaa tcagcatcgt gatttatccc
    49321 aaactcacgg cccgcgagaa tttgctcata aggtagttcc agttcttcga gcgtcagcaa
    49381 tacttttttt acgttggttg aattattccg accccacagc gtaatcatac tcaccccttt
    49441 tccgacaggc agccacaggc tgaacaggac tccgatggtg aagtaaagtt aacttttaag
    49501 caacagctgg caaaaaaatg acaccagaag cagcgaagcg gcaggatatt gcataaactt
    49561 taaaaacttt accaacttac ggtttcttta agtttgtgtg tgcgttatta atcaccgaac
    49621 ttatcatacg gcgatataac gtattttttt gaatggatac tcgggtggca tttatgacgc
    49681 aatactcctc tctccttcgt ggtcttgcag cgggttctgc atttttattc ctttttgccc
    49741 caacggcatt cgcggccgaa caaaccgttg aagcgccgag cgtggatgcg cgtgcatgga
    49801 ttttaatgga ttacgccagc ggtaaagtgc tggcagaagg caatgcggat gagaaactgg
    49861 atcccgcgag cctgactaaa atcatgacca gctatgtggt tgggcaggcg cttaaggccg
    49921 ataagattaa actcaccgat atggtgacgg tcggtaaaga tgcctgggcg acgggaaatc
    49981 cggcactgcg tggttcatcg gtaatgttcc tcaaaccggg cgatcaggtt tcggtggcag
    50041 acttgaacaa aggtgtgatt atccagtccg gtaatgacgc ctgtattgcg ctggccgatt
    50101 acgttgccgg gagccaggag tcatttattg gtttgatgaa tggttatgcc aaaaaactgg
    50161 ggctgaccaa cactaccttc cagacggtgc acggtctgga tgcgccgggg cagttcagta
    50221 ccgcgcgcga tatggcattg ctgggtaaag cattgatcca cgatgtgccg gaagagtacg
    50281 ccattcataa agagaaagag ttcaccttca acaaaattcg tcagcctaac cgtaaccgtc
    50341 tgctgtggag cagcaatctg aatgttgatg gcatgaagac aggaaccacg gcaggcgcgg
    50401 gatataatct ggttgcttcg gctacccagg gcgatatgcg tttaatctcc gtagtgctgg
    50461 gggcgaaaac cgaccgtatc cgttttaatg agtctgagaa attattgacc tggggtttcc
    50521 gcttctttga aaccgtgacg ccaattaaac ctgatgccac ctttgtgact cagcgcgtct
    50581 ggtttggtga taagagcgaa gtgaatctcg gggcaggcga agcgggctca gtgaccatac
    50641 cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg cagcttaccg
    50701 caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac ggtaaatcca
    50761 ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc tttggtcgga
    50821 tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc tcttaatctt
    50881 ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca tcaacttaat
    50941 acgctgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcgc tcaccacaat
    51001 atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt tactgtggtc
    51061 aacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg gcaactcttc
    51121 aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcagaat aaaaagcgat
    51181 atccgggcaa aaattattca gcgtttgctg aaaatcgatg ggtttgaaaa tggcgttgct
    51241 ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttttctt tcagcgccag
    51301 aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa tcgcttcaat
    51361 aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt ctaccagtgt
    51421 cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt gatcgcttaa
    51481 caggtaatgg ctggcgctgc gcggttccag aacaatatag ccgccgagca aaacgacggg
    51541 cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa gcagggcggc
    51601 ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct gcccgatacg
    51661 ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc cgctcaatac
    51721 tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta atgcaaaaca
    51781 gaagcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt gccagataat
    51841 ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat ccagcggcca
    51901 gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca ttaaaagtga
    51961 gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa tcaccgtacc
    52021 gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc cgatattttc
    52081 gacaaacggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacca acaggctgac
    52141 cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc cccacaacca
    52201 aagtaccgcg gccagcagcg gcaccacggt aatcaaatct ttagcaataa aaatcgccaa
    52261 cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata gagagagatt
    52321 caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa tacaggcaag
    52381 tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat ttccctcaag
    52441 cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt aaagaaagcg
    52501 tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta caaaattcgc
    52561 tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga agaaattgca
    52621 tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt ttccctctct
    52681 gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgattcaa cccggtatgt
    52741 tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg atgaccgcgt
    52801 atctggcggg cgggatgttt ttacaatggc tcctggggcc gttgtcggat cgtattggtc
    52861 gccgtccggt gatgctggcg ggtgtggtgt ggtttattgt cacctgtctg gcaatattac
    52921 tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata agcctctgtt
    52981 tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg gtttgtatca
    53041 agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt ccactggtgg
    53101 gcgcggcgtg gatccatgtg ctgccctggg aagggatgtt tgtcttgttt gccgcattgg
    53161 cagcgatctc ctttttcggt ctgcaacgag ccatgcctga aaccgccacg cgtataggcg
    53221 agaaactgtc gctgaaagaa ctcggtcgtg actataagct ggtgctgaag aacggtcgct
    53281 ttgtggcggg ggcgctggcg ctgggattcg ttagcctgcc gttgctggcg tggatcgctc
    53341 agtcgccgat tatcatcatt accggcgagc agttgagcag ctatgaatat ggtttgctgc
    53401 aagtgcctat tttcggggcg ttaattgcgg gtaacttgct gttagcgcgt ctgacctcgc
    53461 gccgcaccgt acgttcgctg attattatgg gcggctggcc gattatgatt ggtctgttgg
    53521 tcgctgctgc ggcaacggtt atctcatcgc acgcgtattt atggatgacc gccgggttaa
    53581 gtatttatgc tttcggtatt ggtctggcga atgcgggact ggtgcgatta accctgtttg
    53641 ccagcgatat gagtaaaggt acggtttctg cggcgatggg aatgctgcaa atgctgatct
    53701 ttaccgtcgg tattgaaatc agcaaacatg cctggctgaa cgggggcaac ggactgttta
    53761 atctcttcaa ccttgtcaac ggaattttgt ggctgtcgct gatggttatc tttttaaaag
    53821 ataaacagat gggaaattct cacgaagggt aaaaaaatgc ctgattgctt tgtgcgatca
    53881 ggcattctcg aattaatggt gatggtcgtc aatctggtgt tcgataacca tcccttcacc
    53941 tacgctggca agatggcgaa cataaggatg cgggtggtaa gccggagctg gcgcaggagc
    54001 cacatagacg gtttgcggca cagtcgcgac gctgaccgct tgtggaacgc tgactgaatc
    54061 aggaaccaca accactttgt atccactcgg cacatcgacg gtgacacttt gcgccatcgc
    54121 cacaccagta aagcccatta aaagcgcgcc caacagtaga caatttttca taatcatttc
    54181 gcctgaaata atgcagcggg ttaatttccg cgaattatgc agccttcatt caggtattgt
    54241 gttgccgggc gtgctgccgt ttttattggt caaatggcgc ttcgtgttta agaactttat
    54301 cgatcacatc cagtacgcct tcacggttat tggagcctgc ccggtatttt gccgctgcga
    54361 cgaccgcgtt gccggcattt tccattgcaa aactaaagcc tgcctgacgc agcatctcaa
    54421 tatcgttacc gccatcgcca aagaccacca cttcgctgtc gtctattccc cataatttct
    54481 gcagttggcg aaggccattg gctttatgta cgccggggat aatcagatcg atgctgccgt
    54541 tgccggtgtg gaccggcacc ataatatcgc cgatggcctc atgtaatgtt ttttgtactt
    54601 gtggaatcag ttcatcggaa agattcaggc caaacttaaa gaagatatct tctaagttgt
    54661 caaagttatc gacgtattcc agacggtgat aatacatttc cgccaccatt ttcatggcat
    54721 cgtcatattt tttgagcgta taggcgctat tttttccgca ggcaataatt tccacttccg
    54781 ggcgcgtcag caaatgttcc acgacagtag taaacgcatc cttcgacagc tcgccattaa
    54841 aaacatcttt gccttcactc actacccagc cgccgttttc agccacaaag gcaatttcat
    54901 tagcaatttc agggaagaaa gagatcaact gataatattg attcccgctg gcgaccacaa
    54961 agcgaatgcc ttgtgctttc atttgctgat actgagccat aaaccgctca cggttatagg
    55021 ttttttgatc gcttaagaaa gtaccatcca tgtctaccgc aattaattta atgctcatca
    55081 actattctcc atcgccgtct gcgttttggt atcgggtttg gcgacggctt tcgcgacaat
    55141 agccgcgaga ataaccagcg ccagtacaac cagcattgca ctacgtaatc cataatgttc
    55201 gccgagatag cccagcagcg gcggcccgac gaggaaagcc agataaccgg tcgttgctac
    55261 cacactgacg cgggttggtg catcggggcc ggtatcgctg gcggcagaaa tggtcagcgg
    55321 gaagcccagc gaggcaccca gtccccagag tacaacagac accccagcga cccaggcgct
    55381 atcgacaaaa ataatcagcc caatacccaa cgcccccatt agcgcactgg cccgaaccac
    55441 ggcaacgcga ctgtaacggt cgatgaacca accgccagtg aagcgtccaa cggtcatccc
    55501 cagggtaaaa ccggcataaa tcagcgagcc ggaagtgggg ctaaaaccgt gaccgtcaac
    55561 cattaataag ggtaaccagt cgttggcaga accttcggca aaggccatcg ccagcaccac
    55621 aacaccaatc agcagcaact ggatatcgcg ataaaaaggt acgccttttt cgccatgctg
    55681 ggtgccatcg gcagcatttt tgcccgtacc gtcagggatt gcctgaatgg cgatatatat
    55741 aggcgcgatg cctaccagcg ccgccaataa aatgtgcacc gttgccggaa cgccaaaggc
    55801 cgtcagtgcc atcccgacac cagcgcctgc cagcgtgccc aggctataaa aaccgtgcat
    55861 catcggcaaa accgttttat tcatttctcg ctcaacggcg gcaccttcaa cgtttatcgc
    55921 cacttccgca gaaccaaaac ttgccccaaa gacgccgaga ccaacggcaa agagcagggg
    55981 cgatgtcagc cagagtgcca gacttaatat catcatcccg accaatgcgc aggacatggt
    56041 gaccaggatg acattacgtg tcccaaagcg tttcactaac cacgccgagc agagaatacc
    56101 gctcatcgaa ccgatcgaca gaccaaagag aacaccgccc atttcagcga tcgagacaga
    56161 gagaatatcg cggatagcag gcgtacgggt tgcccaggac gccattaaca ggcccggcaa
    56221 aaagaagaac ataaacagcg cccaggttcg gcgtttcaat gcattacgtg aagaatttac
    56281 ggtcatagat cacgtcaaaa taagaagagg gaagacaaca ttagcaaggt tgtgtacatt
    56341 tgtacacaat tgcagacaga ggaaatgaca tgcgtcgcgc taacgatccg caacggcgag
    56401 aaaaaattat ccaggccacg ctggaggcgg tgaaacttta cggaatacat gctgttacgc
    56461 accgcaaaat tgctaccctt gccggggtac cgttgggatc gatgacctac tatttttcag
    56521 gaattgatga gttgttgctg gaggcgttca gcagttttac tgagatcatg tcccggcaat
    56581 atcaggcatt ttttagcgat gttagtgatg ctcaaggcgc atgccaggct atcaccgata
    56641 tgatctacag ctcacaggtt gcaacgccgg ataacatgga gctgatgtac cagctctacg
    56701 cgctggctag ccgaaaaccg ctattaaaaa cggtaatgca aaactggatg cagcgcagtc
    56761 agcaaacgct cgaacaatgg tttgaacccg gaaccgcccg cgcgcttgat gcgtttattg
    56821 aggggatgac gctgcatttt gtcaccgacc gtaagccgct atcgcgcgag gagattttga
    56881 ggatggttga gagggttgca gggtagtaga taaatttcag gcaacaaaaa acccatcaac
    56941 cttgaaccga aatggcgggg ttgatgggct ccacaaaatg gggacatcaa agaaaagcag
    57001 tggcactaat taagactgat gccctgcgga aaagttctgc ggttgtgcaa aaaaatttca
    57061 ttttcagggc aacttcagtt ttatcctaat cctggccata ccatgacgat gattgtccct
    57121 gccagcgtca gcaggacgtt ggcgattgcg taggtgcccg catagcccag cgccgggatg
    57181 ttactgcgag ctgtatcact gatgatctcc attgccggcg cgcaggtgcg tgcgcccatc
    57241 attgcgccga acaacagtgc gcggttcatt cgcaatacat aagcaccgaa caagaaacag
    57301 ataaccacgg gcacaagact gacaatcaat ccggcaatca acatctgacc gccaatcgcg
    57361 cccagaccgt tattaatacc gctaccggcg ctcagaccaa cgcctgccat aaacaccatc
    57421 aagccgaact ctttcaccat gcttaatgcc ccttgcggaa tgtaaccgaa ggtcgggtgg
    57481 ttagcacgca taaagcccag cataattccg gcgaataaca acccggcagc gttccccatg
    57541 ccgaaactga atgtgctgaa ctggaaggtg atcatcccga tcatcagccc aataacaaag
    57601 aaggcgcaga atgccagcag gtcagtgacc tggctgtgaa tcgagataaa gccgatgcga
    57661 tcggcgatgg tttttacgcg gcgggcatcg ccgctgactt gtaaaacgtc acctttgtta
    57721 agcacgacgt tgtcatcaat cggcatctca atctggctac gaatgacgcg gttaaggaag
    57781 caaccgtgat cggtcaactt cagttgtgcg agacgtttac ctacagcgtt atggttttta
    57841 acgaccactt cttcagtgac gatacgcatg tcgagaaggt cacgatcgaa aacttcttta
    57901 ccgttacgga agctgggatc gagtcgggca tgggcgtcgg gatagcctac caacgctatt
    57961 tcatcgccca tttgtagcac ggcatcaccg tctggatttg ccagaatccc gttacgtcga
    58021 atacgttcaa tgtagcagcc ggtttgtcga taaataccca gttcacgcag atttttgccg
    58081 tcggtccagg ccaccagctc cgggccgacg cgataggcgc ggatcaccgg taaataaacc
    58141 ttacggttgg catcagtgtc caggccacgt tcgcgggcga tttgctgggc gctggtctgt
    58201 aagtcctgat gctgcaattt cggcaagtaa cgcgcaccaa caatcaaact caccagaccg
    58261 attaaatagg ttaaggcata cccgaggctc agattatcca gtgccagtga gagctgcctg
    58321 ctttccatac cggaatgacg cagtgtatcg ccagcaccga ccagaaccgg tgtcgacgtc
    58381 atagagcctg ctaacatacc ggccgtcagg ccaatatccc agccaaacag cttacctaac
    58441 cctaaggcga tcaccagcgc actgccaacc atcaccagtg ctaacattag gtaatttttc
    58501 ccatcgcgaa aaaaaatgga aaaaaagttc ggtccggctt cgaccccgac gcagaaaata
    58561 aacagcataa agccaagatt aagcgcatcg gtgttaatgc tgaaatgttg ttggcctaat
    58621 aacagcgata cgactaaaac gccaatggaa ttacccagtt ggatcgaacc aagtcgtaac
    58681 tttccgagac atagcccaag cgcgaggacc acaaataata acagaatgta attcccattt
    58741 aacaattcgg cgacgtttat attcacggag gctaacttct tgtttactag taagctgttg
    58801 aaagaaatgg taatttacga taatgttttt taccagaatt cagggcgcag attcattcag
    58861 cgcacctaaa cgatagtaaa gtaacaatat attttactag tgtaatcaca ttaggtatca
    58921 acggctatat gaattgcgtt ggcctatatt agcatggaat gcgaagcggc tttatcttac
    58981 tgaacgccac actggcgaaa aatgtgttcg atagacgcag tgtcaggagg aacgagtgaa
    59041 acataaacaa cgttgggcgg gggcaatctg ctgttttgtc ctcttcattg tggtgtgcct
    59101 ttttctggcg acgcacatga aaggcgcttt tcgggctgcc gggcatcctg aaatcggctt
    59161 gctgtttttc attcttcctg gagcagttgc cagtttcttt tcacagcgta gagaagtcct
    59221 gaaacctcta tttggcgcaa tgctggcggc accctgttca atgcttatta tgcggctgtt
    59281 tttttcaccg acccgctcat tctggcaaga gctggcatgg ttactaagcg cggtgttctg
    59341 gtgtgcgctg ggggcactgt gtttcttatt tatcagtagc ttgtttaaac cacagcacag
    59401 aaaaaatcag taaagccctc aacgcgaggg cttgtcagac gatcaggcgt ccagattctc
    59461 tttcacccat gcagcaaaat cggtatagcc gccgatatgt tgctgatcga caaaaatctg
    59521 cggcacggtt tctacgggtt tacctgcctt ttgttgtaga tcttctttag tgatcccttc
    59581 cgcacgaata tctacatact gatactgaaa atcatcgcgt tcattgctca atttctcagc
    59641 cagatctttt gcacgcacac agtaagggca acccggacga ccaaaaataa cggtttgcat
    59701 tatttctctc ctcatagatt tatgcctgta atgatcacgc taaaatgtat tcgctgaaag
    59761 taggtttaac ctgttgcatt aattgctaaa agctataact gttaaacaca atacagtgaa
    59821 aagttttaga ctgaaggttc actttgcaga gggaagcgta tgcgcgcgat cggtaaattg
    59881 cctaaaagcg tgttgatact ggaatttatc ggaatgatgt tgctggcggt ggcgctgctg
    59941 tcggtaagcg actccctgtc gctgcctgag ccattttctc ggccagaagt gcagattctg
    60001 atgatttttc tcggtgtttt gctcatgctt cccgctgcgg tggtggttat tcttcaggtg
    60061 gcaaaacgtc ttgccccaca gctgatgaac cgtccaccgc agtattcacg ttcagaaaga
    60121 gaaaaagata atgacgccaa ccattgaact tacttgtggc catcgctcca ttcgccattt
    60181 cactgatgaa cccatttccg aggcgcagcg tgaggcgatt attaacagcg cccgtgcgac
    60241 gtccagttcc agttttttgc agtgcagtag cattattcgc attaccgaca aagcgttacg
    60301 tgaagaactg gtgacgctga ccggcgggca aaaacacgta gcgcaagcgg cggagttctg
    60361 ggtgttctgt gccgacttta accgccattt acagatctgt ccggatgctc agcttggcct
    60421 ggcggaacaa ttgctgctcg gtgtcgttga tacggcaatg atggcgcaga atgcattaac
    60481 cgcagcggaa tcgctgggat tgggcggggt atatatcggc ggcctgcgca ataatattga
    60541 agcggtgacg aaactgctga aattaccgca gcatgttctg ccgctgtttg ggctgtgcct
    60601 tggctggcct gcggataatc cggatcttaa gccgcgttta ccgtcctcca ttttggtgca
    60661 tgaaaacagc tatcaaccgc tggataaaga cgcactggcg cagtatgacg aacaactggc
    60721 tgaatattac ctcacccgtg gcagcaataa tcgccgggat acctggagcg atcatatccg
    60781 ccgaacaatc attaaagaaa gccgcccatt tattctggat tatttgcaca aacagggttg
    60841 ggcgacgcgc taaaaccgcc acgccgatgt atgatacgcg ggcttttgac caggtctgac
    60901 agagaggtgc agggtgaaaa ttgccatatt gtcccgggat ggaacgctct attcgtgtaa
    60961 gcggctgcgt gaagccgcta tacagcgcgg tcacctggtt gaaattcttg atccgctttc
    61021 ttgctacatg aacataaatc ctgcggcgtc ttctattcac tacaaaggcc gcaagttacc
    61081 ccattttgac gcagtgatcc cgcgtattgg caccgccatt accttttatg ggacggcggc
    61141 actgcgccag ttcgagatgc tggggagcta tccgctcaat gagtcggtcg ccattgcccg
    61201 ggcgcgtgac aaattgcgtt ccatgcaact gctggcgcgt cagggcatcg acctgcctgt
    61261 cacgggcatt gcgcattcgc cggatgatac cagcgattta atcgacatgg tcggtggtgc
    61321 gccgctggtg gtcaagttgg ttgaaggcac gcagggaatt ggcgtcgtgc tggcggagac
    61381 gcgtcaggcg gcggaaagcg tgattgacgc tttccgcggt ctgaacgcgc atattctggt
    61441 gcaggaatat atcaaagagg cgcaagggtg cgatatccgc tgtctggttg ttggcgatga
    61501 agtggtcgct gcgattgaac ggcgggcgaa agagggcgat tttcgttcca atttgcatcg
    61561 tggcggcgcg gcaagcgtcg ccagtatcac accacaggag cgtgaaatcg cgataaaagc
    61621 cgcgcgaacg atggcgctgg acgttgctgg tgtggatatt ctgcgtgcta atcgcgggcc
    61681 gttggtgatg gaagtgaatg cgtcgccggg gctggaagga atagaaaaaa ccaccggtat
    61741 cgacatcgcg ggtaaaatga tccgctggat cgaacgccac gctacgacag aatattgcct
    61801 gaaaacgggt ggttagtcgc aatcacatta ctgatcatgg ttttgcctgc tctttttgcg
    61861 taagctgtgc cggtcttttt aacgaaagag gttgtacaaa attatgacat cgctggtcgt
    61921 tcctggtctg gatacgctgc gtcaatggct cgatgacctg gggatgagtt tttttgaatg
    61981 tgataactgt caggctctgc atctgcccca tatgcagaat ttcgacggtg tctttgatgc
    62041 caaaatcgat ctgatcgata acacgatcct gttttctgcc atggcggaag tccgaccttc
    62101 agccgtattg ccgctggcgg cggatttatc tgccatcaat gccagttcgc tgaccgtgaa
    62161 agcatttctt gatatgcagg atgataatct gccaaagctg gtggtttgcc agtctttatc
    62221 cgttatgcag ggcgtaacct atgagcagtt tgcatggttc gtgcgtcaga gcgaagagca
    62281 gatttcgatg gtcattcttg aagctaatgc ccatcaactg ctgttaccga ctgacgatga
    62341 agggcaaaac aacgttaccg aaaactattt cctccactga taactccttt cgagcacgca
    62401 gtcgctggtg cagtggctgc gcgctgcaaa attatctgct gtttttaacc ttttcttaaa
    62461 gattatttca cttctcttgt gtcgatttgg ctttatcaca tagagcaaat acgcataaaa
    62521 atttgttaaa taccgttttt taatccgagc tatagtctca aaccctggct aaagttattc
    62581 ttgcgatgct tttatatagc gagcagtgct ggccgggaga gagttctctt ttcttacacc
    62641 gcgccgataa aaaatatgca cgtttattgc atatctttca gtgtgacaac ttttgttcgt
    62701 ttgttaacga actttcagaa ggaaagagat atgaccgcct taaataaaaa atggctttcg
    62761 ggtctggttg cgggtgctct gatgggcgtc tctgtcggca cgctcgcggc tgaacaaaaa
    62821 acactccaca tttataactg gtctgattat atcgcccccg acacggtggc taactttgaa
    62881 aaagaaaccg gtattaaagt cgtctacgat gttttcgact ctaacgaagt actggaaggc
    62941 aaattaatgg ccgggagcac cggctttgac ctggtggtgc cgtcagcaag ctttcttgaa
    63001 cgccagctga cagcgggagt tttccagccg ctggataaaa gcaaattgcc ggagtggaag
    63061 aatctcgacc cggaactgct gaagttggtc gccaaacacg atcccgacaa taaatttgct
    63121 atgccctata tgtgggcgac gaccgggatt ggctataacg tcgataaagt caaagcggtg
    63181 ctgggcgaaa acgcgcccgt cgatagctgg gacttgatcc tcaagcctga aaatctggaa
    63241 aaactgaaaa gctgcggtgt atctttcctc gatgcgccag aagaagtttt tgctaccgtg
    63301 ctgaattatc tcggcaaaga tcccaacagc actaaagcgg atgattacac cggaccggct
    63361 accgatctgc tgttaaagct gcgcccgaac attcgttatt tccattcatc tcaatatatt
    63421 aacgacctgg caaacggcga tatttgcgtc gctatcggct gggcaggtga tgtctggcag
    63481 gcgtcaaacc gcgcgaagga agcgaagaat ggcgtgaatg tctcgttctc gattccaaaa
    63541 gaaggggcga tggcgttctt tgatgtattc gccatgcctg cggatgccaa aaataaagac
    63601 gaagcctatc agttcctgaa ttacctgctg cgtcctgatg tggtggcaca tatctctgac
    63661 catgtgttct atgctaacgc caataaagca gccacgccgc tggtgagtgc ggaagtccgt
    63721 gataacccgg gtatttatcc gcctgcggat gtccgcgcga agttgttcac tctgaaagta
    63781 caggctccga aaatcgaccg tgtgcgcacc cgcgcgtgga ccaaagtgaa gagcggaaaa
    63841 taatccgcag ccttagatgc cgggcgggag cgccactccc tccggcaatt cgcaccaaca
    63901 tggtgcgctt gcacacattt aatgccggag agcagccgtg aatgacgcta tccctcgccc
    63961 ccaggcgaaa acccgtaagg cgctgacccc gctattagaa atccgcaatc tgaccaaatc
    64021 ctacgatggt cagcatgcgg ttgatgatgt cagcctgacc atctacaaag gtgaaatctt
    64081 tgcgctgctg ggcgcatccg gctgtggcaa gtccacgctg ctgcgtatgc tggcaggttt
    64141 cgaacaacct tcagccgggc agattatgct tgatggcgtc gatttgtcac aggtgcctcc
    64201 ttacctgcgc cccatcaata tgatgtttca gtcttacgcg ctgtttccgc atatgaccgt
    64261 ggaacagaac atcgcttttg gcctgaaaca ggacaaacta ccgaaagcgg aaattgccag
    64321 ccgggtcaat gagatgctcg gccttgtgca tatgcaggag ttcgccaaac gcaaaccgca
    64381 tcagctttcc ggtggtcagc gacagcgtgt ggcactggcc cgaagccttg ccaagcgccc
    64441 gaaactatta ctgctcgacg agccgatggg cgcgctggat aaaaagctgc gtgaccggat
    64501 gcagcttgaa gtggtggata ttctggaacg cgtcggtgtg acttgtgtga tggtcaccca
    64561 cgatcaggaa gaggcgatga ccatggcggg gcgtatcgcc attatgaatc gtgggaaatt
    64621 tgtccagatt ggcgaaccgg aagagatcta cgagcatccg actacccgct atagcgccga
    64681 atttattggc tcggttaatg tctttgaagg tgtactcaaa gagcgtcagg aagatggtct
    64741 ggtgctttat tcgccggggc tggtgcatcc actgaaagtc gatgcggatg cttcagtggt
    64801 cgataacgtg ccggtacacg tagcgctgcg tccggaaaaa atcatgcttt gcgaagagcc
    64861 gcccgccaat ggttgtaact tcgcggtggg ggaggtgata cacattgcct atctcggcga
    64921 tctttcggtg tatcacgttc gtctgaaaag tgggcagatg attagcgccc agctacaaaa
    64981 cgcccatcgt catcgtaaag ggttaccgac ctggggcgac gaagtgcgtt tgtgctggga
    65041 agtggacagc tgtgtggtgc tgacggttta aggagcaaag atgagtacac ttgaacctgc
    65101 tgcccagtcg aaaccgccgg gcggatttaa gctgtggttg tcgcagttgc aaatgaagca
    65161 tgggcgcaaa ctggtcattg cgttgccgta tatctggttg atcttgctgt ttctgctgcc
    65221 atttctgatt gtctttaaaa taagcctggc ggagatggcg cgcgctattc caccttatac
    65281 cgaactgatg gagtgggctg acgggcaact gtccatcacc cttaatttcg gtaatttcct
    65341 gcaactgacc ggcgatccgc tctatttcga tgcttatctc cagtcgttac aggtggcggc
    65401 gatttcgaca ttttgctgtt tactgatcgg ctatccgctg gcgtgggcgg tggcgcacag
    65461 taagccttcg acccgtaata tcttattact actggtgatc ctgccgtcgt ggacctcgtt
    65521 tctgattcgt gtctacgcct ggatgggaat attaaaaaac aacggtgtgc tgaataattt
    65581 tctgctgtgg ctgggggtta tcgatcaacc gctgaccatt cttcatacca atctggcagt
    65641 ttatattggc attgtttacg cctacgtgcc gtttatggtg ctgccgattt ataccgcgtt
    65701 gattcgtatt gattattcgc tggtggaagc agcgctggat ctcggtgcac gaccgctgaa
    65761 aacgttcttt actgtgatcg tgccgctgac taaaggtggg attattgccg gatcgatgct
    65821 ggtgtttatc ccggctgtgg gcgagtttgt gatcccggaa ctgctcggtg gcccggacag
    65881 catcatgatc gggcgcgtgc tatggcaaga gtttttcaat aaccgcgact ggccggtggc
    65941 ctcggcggta gcgatcatca tgttgctgct gctaattgtg ccgataatgt ggtttcacaa
    66001 acaccagcaa aaaagcgtgg gagaacacgg atgaataatt taccggtagt tcgttcgccc
    66061 tggcggattg tgattttgct gttgggcttc acctttctct acgcgccaat gctgatgctg
    66121 gttatctatt cgtttaacag ctcgaagctg gtgacggtgt gggccggctg gtcaacgcgc
    66181 tggtatggcg aattattgcg cgatgacgcg atgatgagtg cggttggttt aagtctgaca
    66241 attgcggcct gtgcggcgac ggcggcggcg atcctcggaa ctattgcggc ggtggtgctg
    66301 gtgcgctttg gcaggtttcg cggatcaaat ggctttgcct ttatgatcac cgcgccgctg
    66361 gtaatgccag atgtcatcac gggcttgtcg ctgttgttat tattcgtcgc gcttgctcat
    66421 gccattggct ggcctgcgga ccgcggtatg cttaccatct ggctggcgca tgtcactttc
    66481 tgtacggctt atgtggcagt cgttatttcg tcgcgtcttc gggaactgga tcgctcgata
    66541 gaagaagcag cgatggatct cggtgcgacg ccgctgaaag tgtttttcgt cattacgcta
    66601 ccgatgatca tgcccgcggt catttctggc tggttactgg cttttacttt gtcgcttgat
    66661 gatctggtga tcgccagctt tgtatccggg ccgggagcca ccacgttacc gatgctggtc
    66721 ttttccagcg tgcggatggg ggtgaatccg gaaatcaacg ccctagcaac attgatcctc
    66781 ggcgcggtcg gaattgtcgg atttatcgcc tggtatctga tggctcgcgc agaaaaacag
    66841 cggatacgcg acatccagtg tgcaagacgt ggctgaagac actaaaattt gccaacctgg
    66901 ctacataatg ccgcgtatgt cgcggcattg ttttcatgga agacgaaacg ttgggatttt
    66961 ttaagaaaac atcttcatct catgctcgcc tgaatgtgcc tgcgcttgtg caggtggcgg
    67021 cgctcgccat tattatgatc cgtggcctcg acgtgctgat gattttcaat acgctgggcg
    67081 tgcgcggtat tggcgagttc attcatcgca gcgtacaaac ctggagttta acgctggtct
    67141 ttttaagcag tctggtgctg gttttcattg agatctggtg tgcgttttca ctggtgaaag
    67201 ggcgtcgctg ggcgcgctgg ctgtatctgc tgacacaaat cactgccgca agttacttgt
    67261 gggcggcttc gctggggtac ggttatccgg agctgttcag cattcccggt gaatcaaaac
    67321 gtgaaatctt ccatagcctg atgctgcaga agctgccgga tatgctcatc ctgatgctgc
    67381 tgttcgttcc ctcgaccagt cggcggttct tccagttgca ataatgtgta taatcgtcgc
    67441 ccctgatgat gtgaaggtca atgtatgcag tgcgcacttt acgacgcggg tcgctgtcgt
    67501 tcctgtcagt ggataacgca gccgattcca gagcaactct ccgctaaaac cgccgatctt
    67561 aaaaatctgc tcgccgactt tccggttgag gaatggtgcg cgccggtgtc aggcccggaa
    67621 caagggtttc gtaataaagc caaaatggtg gtgagtggta gcgttgaaaa accactgctc
    67681 ggtatgctgc atcgcgacgg cacaccggaa gacctttgtg actgcccgct ttatcctgcc
    67741 tcatttgcgc ccgtttttgc ggcgctaaaa ccgtttatcg cccgagcggg gttaacgccc
    67801 tacaacgtgg cgcgtaagcg tggcgaactg aaatacattc tgctgactga aagccagagc
    67861 gatggcggca tgatgctgcg ctttgtactg cgttctgaaa ccaaactagc acaactgcgt
    67921 aaggcattgc cgtggttaca ggcacaattg ccgcagctaa aagttatcac cgtcaatatt
    67981 cagccggtac atatggcgat tatggaaggg gagacggaga tctacctgac cgaacaacag
    68041 gcgctggtag agcgttttaa cgatgtgccg ctgtggatcc gtccgcaaag tttcttccag
    68101 actaatccgg cggtcgccag ccagctgtac gctaccgcgc gcgactgggt acgacagctg
    68161 ccggttaaac atatgtggga tctgttctgc ggtgtggggg gctttggttt acactgcgcg
    68221 acgcctgaca tgcagttaac cgggatcgaa attgcgctag aggccattgc ctgtgcaaag
    68281 cagtcagccg ctgaactggg cttaacgcgt ctgcaatttc aggcgctgga ctccactcag
    68341 tttgccaccg ctcaggggga ggtgccggag ctggtgctgg ttaacccgcc gcgccgcggc
    68401 attggtaaac cgctgtgtga ttatctctca acgatggcac cgcgttttat tatctactcc
    68461 agctgtaacg cccaaaccat ggcgaaagat atccgcgaac tgccaggtta ccgcatcgaa
    68521 cgggtacagc tttttgatat gttcccgcac accgcgcact atgaagtgct gacgctgctg
    68581 gtaaaaatgt aaatacatca ttcaaatcaa tatcacctcg gcatacatcc atccaaaata
    68641 ataatcgtga tcgatcgatt aataataacc gatcgatcac ggttatatat tgctctattt
    68701 ttgcaaatca aatcacaaat aatcaaataa gatctttaat tgaactgttt tgattactac
    68761 agtgcactta ccgaatacat acagaggcat tgcttagcga aacacaaaat ttcctgagac
    68821 attattaacg tcagcagaag gttcatgcat tgacaacgga aatgtacgcg tatggagggc
    68881 gttccgacga tgtttgccaa attcatagat gttattcaaa cgtttttaac tgaaccggca
    68941 attttaattg gtttgctggt gggtattggt tatgcccttg ataagaaatc gccaataaaa
    69001 attattaccg gcatggtcag cgccatggtg ggattaatga tggtgttatt tggtggtttc
    69061 cagttttccg caacatttaa accggtagca gaagccgtaa gtaaagccta tggtgttcac
    69121 ggatatttaa tggattccta cgcaatgaaa gcggcaacgc aaattgcatt gggggataat
    69181 tttggttatg tcgggtatgt atttgtactg gcgtttttta ccaacctgct gctggtatta
    69241 tttggtcggt atactggtgc caaagggata tttttgacag ggaataccgg tgtttcacat
    69301 tctcaggcgg tattatggtt gattgtcttt tggctgggtt ttagctggac tacatccatt
    69361 attattgccg gaatattaac gggtgtattc tgggcgttct ccaccacgct cattgttaag
    69421 cccattgcca aagttaccaa agatgccggt tttaccattg ctcataacca gatgttggga
    69481 ttgtggtttt tctccaaatt cgcccataaa tttggtgacc ctgaaaagca cgatgctgaa
    69541 aacctgaaac ttcccgggtg gctggcgatt tttaaccata acgtaacggc tatcgccatt
    69601 gtgatgacgc tgtttgtcgg tggtttcttg ctatcaacgg gtatcgataa tgtccagtta
    69661 atggcgaaag gcaagccctg gtacatctat atcatcaacc ttggcttaca gttctccatg
    69721 tatatggtca ttctgctgca gggtgtgcgc atgatggtgg gcgaaattaa cggttcgttt
    69781 aaaggctggc aagatcgctt tattcctaac gctattcctg ctgtcgatgt tgcagcgctg
    69841 ttaccttttt cacccaatgc cgcaacgtta ggcttcgtct tctgtacttt tggcaccatt
    69901 ttttcgatgg ggatcttatt actggttcac agcccaatta tggtattgcc tggctttgta
    69961 cctctgtttt tctctggcgg tccaattggc gtattagcaa accgcatggg cggataccgt
    70021 tccgtaatta tctgtacgtt cttgctgggt attattcaga ccttcggtac ggtgtgggct
    70081 attccgttaa ccgggcttgc tgaaaatggc gtcggctgga caggaatatt tgactgggca
    70141 accgtatggc ctgctatttg tgaagttctg aaatttatcg ctgcaacatt ccatcttggt
    70201 ccttacgcgg gttaattaaa tattaagggt ttaaaaatga aagatacaat taatattctg
    70261 tttgtctgtg gttacggtgt aggcagtagc gttatgttac aaacggtcgt taaaaaagcg
    70321 ctgaccaaat atgatttcaa agttgaaatg gaacataccg ccgcaggtga agtcggcgga
    70381 tttaccgact gggcggatat ttttgctatt tccaaaaaat tactcgatgt ggttagtctt
    70441 gacccgaaac aagggcaata tttgattccg atagagaata ttatggacgg tgaaactatt
    70501 ggtcagcaaa tttatgaagt ggttcagcaa cattttccgc atttaattaa tcaataaggt
    70561 agggcgtaat gagagcgatt attctgctgt tcgatagtct gaataaacgt tatctgccac
    70621 cttatggcga tgcgttaacc aaagcgccta atttccaacg tctggcggct catgccgcca
    70681 cctttgaaaa cagttacgtc ggcagtatgc cgtgtatgcc tgcccggcgg gagttgcata
    70741 ccgggcgatg taacttcctg catcgggagt gggggccgtt agaaccgttt gacgattcca
    70801 tgccggagct gcttaaaaag gcggggatct atacccacct gataagcgat catttgcatt
    70861 actgggaaga tggcggcggg aattatcata atcgctacag ttcgtgggag attgtgcgcg
    70921 gacaggaagg cgatcactgg catgcaagcg ttgcgcaacc gcctattccc gaggtgctac
    70981 gggtgccgca aaagcagacc ggtggcggtg tttctggtct gtggcgccat gactgggcaa
    71041 accgggaata tattcagcag gaagcggatt ttccacaaac taaagtgttt gatgcaggct
    71101 gtgcctttat ccacaaaaat cacgcggaag ataactggtt attgcagatc gaaacgtttg
    71161 atccgcacga gccgtttcac acaacggaag agtatctttc cttgtatgaa gataactggg
    71221 atggaccgca ttatgactgg ccgcgtggcc gggtgcagga aagcgacgag gccgtggagc
    71281 acatccgttg ccggtatcgt tcgctggtgt cgatgtgcga tcgcaacctg gggcgcattc
    71341 tcgacctgat ggatgagcac gatctgtggc gagataccat gctgattgtc ggcactgacc
    71401 acggcttttt actcggtgag catggctggt gggcgaaaaa ccagatgcct tattacaacg
    71461 aagttgccaa taatccgttg tttatctgga acccgcgcag tggtgtaaaa ggagagcggc
    71521 gacaggcact ggtacaaatg atcgactggg cgccaacgtt gtatgacttt tttcaacagc
    71581 cagtgccgcc cgatgtgcag gggcaaccgc tggcgaaaac ggtcagtcac gatgaaccag
    71641 tacgcagctc ggcgatgttt ggtgttttca gtggtcatgc taacgtaact gatgggcgtt
    71701 atgtgtatat gcgtgcagcg ctgccggggc gtgaggatga tattgccaac tacacgttga
    71761 tgtcctgcaa aatgaacagc cgctatccgg tggatgagat gcgggcttta tcgctggccc
    71821 caccgtttcg ttttaccaaa gggttacagg tattacgcat cccggcacag gaaaaatata
    71881 aggggttgaa tcagtttggt catttgctgt ttgatctgca aaacgatccg cagcagctac
    71941 atccgattca tgatgatgtg atcgagtccc ggatgatcgc gttgctgatt cagttgatga
    72001 aagataatga tgcgccgcca gagcagtttc agcgcctggg attagcgtag agaagtgatt
    72061 ttgccggagt gggtgttcgc ccactccggc aaatgaataa ttattgcggg aaccactgat
    72121 cgctgatttg ttgatatgta ccgtcagctt taattgctgc cagcgcgtta ttcagttttt
    72181 ccagcagagc tttgttatcc ggacgtacag cgatacccag accggtgcca aagtattgcg
    72241 gatcggtcac tttttcagta gcaacgccca gttgcggatt ggttttcagc cattcattaa
    72301 ccaccgcagt gtcaccaaat acgccatcaa tacggctatt tttcagatcg ataaaggcat
    72361 tttgataact gtcataggag accgttttca cttccgggtg ctgatcctga atatatttct
    72421 ggtgcgtagt accgttttcc atcccaatgc gtttgccttt caggtcggca aacgttttgt
    72481 aggtatcttt tttggcaatc acgacggctg agttttcgta atagggcgtg gtgaacgata
    72541 cctgcttgct acgatctggt gtgatatcca taccggagat tacggcgtca tatttcctga
    72601 atttcagtga cgggatcagg ctgtcgaacg cgtgattggt aaaagtacat tctgcctgca
    72661 tctgtttgca taacgctttt gctaggtcga tatcaaagcc gacgatctgg ttattggcat
    72721 ccatcgactc gaacggcgga taggtcgctg atacgccaaa attgattttc tctgcggcag
    72781 aagcgccgaa cgcacaggat gccagtaatg cggcaagaac taactttttc atgatggaac
    72841 tcccgtctgt caatcttatg attattggcc gtgtctgcgg catgggataa caatgccatc
    72901 aagtgaattt atatgcaata aatatgatta aataatttgc atgaaataaa aaaagacgga
    72961 caacttagtg agttgtccgt ctttattata agaatttatg cactatgtag gccggataag
    73021 gcgtccacgc cgcatccggc acaggcaccg tgctgatgtc tgatgcgacg ctggcgcgtc
    73081 ttatcagacc tacaaaaccc ccggcgaatg tacgcagcca cattaattcc ttcgctcaaa
    73141 cgccagcgct ttgcgctcga tcagacgcat catcagcgtc agcaggccgt taacgaccag
    73201 ataaataatc cctgccgcgc cgaacaccat gacatcgtag gtgcgtccgt acaacaactg
    73261 gctgtatccc atcacttcca tcagcgtaat ggtgtaagcc agagaggtac ttttgaacac
    73321 cagcaccact tcgttggaat aagaagagag cgagcgttta aaggcatacg gcagcaggat
    73381 cgccagcgta tcttttttgc tcattcccag ggcgctacag gactgccact gaccttccgg
    73441 gatcgcacga attgcaccgt aaaacagctg cgtggtatac gccgcactgt tcagcgacaa
    73501 cgcaatcagc gcacataacc acggttctga caacaaatgc cacagtgccg gatactcctg
    73561 caacgtcggg aactgacccg gcccgtaata aatcaggaag atctgcacca gcagcggtgt
    73621 accggtaaac agcgtgatat aaccccgcac cagccacacc agcaccggcg ttttcagcgt
    73681 caggatgatg gtaaaaatca acgccagaat cagtgccaca atcagcgagg caacggttag
    73741 cgtcaggctg gtgtgcagcc ctttcatcag ttcgggtaaa tactcaaaca ttagctgggc
    73801 ctccgctcaa aacgtgtcgc gcgcaggtca atgcgtttga gaatgtactg actgagcagg
    73861 gtgatcacca ggtaaatcgc cgccgccaca atgtaccagg taaatggttc ctgggtacga
    73921 gtagcgatgc ttttggtttg cagcattaaa tcattcacac taatcaaact gaccagcgcg
    73981 gtatctttca gcagcaccag ccactgatta ccgaggccag gcagcgcatg acgccacatc
    74041 tgcggcatca ccagacggaa aaagatagcc gatttcgaca gccccagcgc ctggccggat
    74101 tcccactgac ccatcggcac cgctttcaac gcgccgcgca gcgtttgcga ggcataggcg
    74161 gcatacagca gtgacagagc gatgacacca caaaggaacg ggctaacgtc gaagttttca
    74221 atctgcatct gcaccgggat ctctacaatc ccaagattga tagtgaagcc atccgaaagt
    74281 gtcagcagca gctgcgagga gccaaaatag ataaacagca ccaccagaat ttctggcagg
    74341 ccacgcagaa tggttaccag cgctgaacct gcccacgcga caggacgcca tttcgcagac
    74401 tcccataccg caaagaacat cgccagcgcc agcccgacaa tcaatgcaca aacggcaagg
    74461 ccgacggtca tcccggcggc gcttgctaaa ggaaaaaatt cattcatcag gaattacttc
    74521 tggaaccatt tgttgtagat ggtttcgtaa gtgccatctt tcttcacttt ttccagcgca
    74581 gtgttgagtt tctgctgcag ctcagtgttg ccctgacgta ccgcgatgcc gaggccagta
    74641 ccgaagtaat ctttatcggt cactttatcg cccactgccg ccagtttcgg gttatctttc
    74701 agccattcag ttaccactgc ggtgtcacca aatacgctgt cgatacgacc gttttgcaga
    74761 tccagttttg cgttctggta gctgtcatac ggcacggtgg tgatttccgg gtgcttatcc
    74821 ataatgaatt tctggtgagt cgtaccattc tgtacgccga cttttttgcc tttcagctga
    74881 tcaacgctgg tgtatttacc ctgctgaccc acaaacaggg cagagttgtc atagtacggc
    74941 gtggtaaaca gtacctgctt ttcacgttcc gcggtgatat ccatcccggc catcaccgct
    75001 tccacgcgac ggaatttcag gcttgggatc aggctgtcaa atgcctggtt agagaaagta
    75061 caggtcgcat caatctcttt acacagcgcc tgtgccaggt cgacgtcaaa accaacgatc
    75121 tggttgtttg catcaatcga ttcaaacgga ggataggagg cttcggtagc aaaacgaatg
    75181 gtttcggcag ctgtggcgga aagactaaaa cctgcaatta acgcggcaat cagaactttt
    75241 ttcattgttg ttatcccgaa tcttagtgag agagatagtt tttaaatgct tcggtttgcg
    75301 gctcggtaaa gcagctcgcg tcgccttgtt ctacgatatg accgttttcc atatacacca
    75361 ctcggctggc ggttttacgc gccacttcaa cttcgtgggt gacgatcacc tgggtaatat
    75421 tcgtttctgc cagctcacga atgatgctga cgatttgtgc cgtaatttcc gggtccagtg
    75481 cggcggtcgg ttcatcaaac agcagtacct gcggttccat cattaacgca cgggcaatag
    75541 caacacgctg ctgctgacca ccagaaagat gcagcgggta acgatcgcta taaggtttga
    75601 gacgcagacg ttccagcagt ttttctgcac gggccagcgc ctgatcttta ctcaacccca
    75661 gtacacggca gggcgcttca atcaggtttt gctgcacggt cagatgcggc cacaggttgt
    75721 attgctgaaa caccatgcca acgttacgac gcaaatcgcg aatcgctttg tctgagggtg
    75781 ttttggtgaa atcgaaatgg ttgcctgcaa tgttgagcgt accggagcgc ggcatctcaa
    75841 gcagattgag tacacgcagc agcgagcttt tacccgcgcc gctggggcca agtaacacca
    75901 gcgtttcgcc ctgtgggcaa tccagcgtga tatcgaacag cgcctgatgc gcgccgtaga
    75961 agcaattaat gccgtttaat tgaatactca ttgacactcg tatactggca gtctgatagc
    76021 tattgaggtc gaagatagta cctttgacag aataattatg caatatttct gctttaaaag
    76081 ttaaaagcaa agcgcattat tcaataaaca tagcacaaaa taacgggggc ggtggtcggc
    76141 gagcataaat gtcggcattc ctcacgaaat gccggacaat ttacggggtt tattggttga
    76201 tcaaggcgtt agcgattctc gatggactga cggagcgtac ccgccgtggc atgaacgcta
    76261 ccgcctaagt agcgcacatc gtcgatcacc caacactggc cttcctgaat cattaacact
    76321 tcatcctgcc agccctggtc accctgcttg agatccacgc gcaatggaat gttacgggca
    76381 tcacgattag ggatagtcga tgcactggca acgtgggcgc tgtctggcaa ggtggttcga
    76441 ctggagaacg gatcactgct taacaattca cgatgactat tatcgcggct ggcatcgcta
    76501 agtagggttg ccagtttgtc actcaaatag gggcgcaggg cggtaatgtc attgctgcga
    76561 tgttgaatgt ggtagtcata gaattgttgg gccacgctgt caggcccccc ctggacacag
    76621 gaaccggtgc ggggaccatt atctttataa gctggcgtga cggtggtaca ggcactcagc
    76681 agcagtgcac atggaataag tagagatagt gaacggtagc gcataatcat ttccttatta
    76741 gcttgctgtc tgcgtcacac tacgaccaaa cgctatcaga ttatttaata cattgttttt
    76801 aatacaattc tgaaacaatc tgaacacctg attgggtttg agtccattgc tggcgaccca
    76861 ctcaaggttg ctcgtagctg acttacttaa attacccaaa caatagacag tgtaaaccat
    76921 ttgtgaggtt gagcgtgtta tatggctgaa atgatcattt aaggtaaata tgatagttct
    76981 gaaatggtgg tataaatcct cctgtagagg ttaagagcat ctgcaagaag ccagtttcac
    77041 atttataacg cgtgataaat gaacgcggtt attgtctaat cattacaaat ttttcacatg
    77101 cgataagaga tcctgctatt aagatgattg ccaaaattac gttgtatttt tgaaaatgag
    77161 cgaaatttga tagtcccagc t
//
LOCUS       DAZFDC010000008        74390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFDC010000008 DAZFDC010000000
VERSION     DAZFDC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.008"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1911)
                     /gene="rnb"
                     /locus_tag="ACHJ48_000629"
     CDS             complement(<1..1911)
                     /gene="rnb"
                     /locus_tag="ACHJ48_000629"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO4706252.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRS"
     gene            complement(1979..3106)
                     /gene="yciW"
                     /locus_tag="ACHJ48_000630"
     CDS             complement(1979..3106)
                     /gene="yciW"
                     /locus_tag="ACHJ48_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO4706253.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(3250..4038)
                     /gene="fabI"
                     /locus_tag="ACHJ48_000631"
     CDS             complement(3250..4038)
                     /gene="fabI"
                     /locus_tag="ACHJ48_000631"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO4706254.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            4516..5082
                     /locus_tag="ACHJ48_000632"
     CDS             4516..5082
                     /locus_tag="ACHJ48_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4706255.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSEEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            5231..6352
                     /locus_tag="ACHJ48_000633"
     CDS             5231..6352
                     /locus_tag="ACHJ48_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4706256.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVAAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHSGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            6352..9454
                     /locus_tag="ACHJ48_000634"
                     /pseudo
     CDS             6352..9454
                     /locus_tag="ACHJ48_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            9458..10831
                     /locus_tag="ACHJ48_000635"
     CDS             9458..10831
                     /locus_tag="ACHJ48_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO4706257.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQRAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            10840..12003
                     /locus_tag="ACHJ48_000636"
     CDS             10840..12003
                     /locus_tag="ACHJ48_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO4706258.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(12055..12861)
                     /gene="sapF"
                     /locus_tag="ACHJ48_000637"
     CDS             complement(12055..12861)
                     /gene="sapF"
                     /locus_tag="ACHJ48_000637"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO4706259.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(12863..13855)
                     /gene="sapD"
                     /locus_tag="ACHJ48_000638"
     CDS             complement(12863..13855)
                     /gene="sapD"
                     /locus_tag="ACHJ48_000638"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO4706260.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(13855..14745)
                     /gene="sapC"
                     /locus_tag="ACHJ48_000639"
     CDS             complement(13855..14745)
                     /gene="sapC"
                     /locus_tag="ACHJ48_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO4706261.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCM
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(14732..15697)
                     /gene="sapB"
                     /locus_tag="ACHJ48_000640"
     CDS             complement(14732..15697)
                     /gene="sapB"
                     /locus_tag="ACHJ48_000640"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO4706262.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(15694..17337)
                     /gene="sapA"
                     /locus_tag="ACHJ48_000641"
     CDS             complement(15694..17337)
                     /gene="sapA"
                     /locus_tag="ACHJ48_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO4706263.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTAWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKLLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKTLSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(17650..17895)
                     /gene="ymjA"
                     /locus_tag="ACHJ48_000642"
     CDS             complement(17650..17895)
                     /gene="ymjA"
                     /locus_tag="ACHJ48_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO4706264.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(18029..19414)
                     /gene="puuP"
                     /locus_tag="ACHJ48_000643"
     CDS             complement(18029..19414)
                     /gene="puuP"
                     /locus_tag="ACHJ48_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO4706265.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(19404..19568)
                     /locus_tag="ACHJ48_000644"
     CDS             complement(19404..19568)
                     /locus_tag="ACHJ48_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706266.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(19717..21135)
                     /gene="puuA"
                     /locus_tag="ACHJ48_000645"
     CDS             complement(19717..21135)
                     /gene="puuA"
                     /locus_tag="ACHJ48_000645"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO4706267.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            21347..22110
                     /gene="puuD"
                     /locus_tag="ACHJ48_000646"
                     /pseudo
     CDS             21347..22110
                     /gene="puuD"
                     /locus_tag="ACHJ48_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            22137..22694
                     /gene="puuR"
                     /locus_tag="ACHJ48_000647"
     CDS             22137..22694
                     /gene="puuR"
                     /locus_tag="ACHJ48_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO4706268.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIILTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            22844..24331
                     /gene="puuC"
                     /locus_tag="ACHJ48_000648"
     CDS             22844..24331
                     /gene="puuC"
                     /locus_tag="ACHJ48_000648"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO4706269.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLIEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIQEGESKGQLLLDGRNAGLAVAIGPTIIVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            24333..25613
                     /gene="puuB"
                     /locus_tag="ACHJ48_000649"
     CDS             24333..25613
                     /gene="puuB"
                     /locus_tag="ACHJ48_000649"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO4706270.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSDDLARSLIPKNY
                     GVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            25651..26916
                     /gene="puuE"
                     /locus_tag="ACHJ48_000650"
     CDS             25651..26916
                     /gene="puuE"
                     /locus_tag="ACHJ48_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO4706271.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERAMQLGQRLTKTL
                     IDAKENVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(27047..28024)
                     /gene="pspF"
                     /locus_tag="ACHJ48_000651"
     CDS             complement(27047..28024)
                     /gene="pspF"
                     /locus_tag="ACHJ48_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO4706272.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            28191..28859
                     /gene="pspA"
                     /locus_tag="ACHJ48_000652"
     CDS             28191..28859
                     /gene="pspA"
                     /locus_tag="ACHJ48_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO4706273.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            28913..29137
                     /gene="pspB"
                     /locus_tag="ACHJ48_000653"
     CDS             28913..29137
                     /gene="pspB"
                     /locus_tag="ACHJ48_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO4706274.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            29137..29496
                     /gene="pspC"
                     /locus_tag="ACHJ48_000654"
     CDS             29137..29496
                     /gene="pspC"
                     /locus_tag="ACHJ48_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO4706275.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            29505..29726
                     /gene="pspD"
                     /locus_tag="ACHJ48_000655"
     CDS             29505..29726
                     /gene="pspD"
                     /locus_tag="ACHJ48_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO4706276.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            29801..30115
                     /gene="pspE"
                     /locus_tag="ACHJ48_000656"
     CDS             29801..30115
                     /gene="pspE"
                     /locus_tag="ACHJ48_000656"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO4706277.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            30325..32003
                     /gene="ycjM"
                     /locus_tag="ACHJ48_000657"
                     /pseudo
     CDS             30325..32003
                     /gene="ycjM"
                     /locus_tag="ACHJ48_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            32017..33309
                     /gene="ycjN"
                     /locus_tag="ACHJ48_000658"
                     /pseudo
     CDS             32017..33309
                     /gene="ycjN"
                     /locus_tag="ACHJ48_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            33330..34211
                     /gene="ycjO"
                     /locus_tag="ACHJ48_000659"
     CDS             33330..34211
                     /gene="ycjO"
                     /locus_tag="ACHJ48_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4706278.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVIFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            34198..35040
                     /gene="ycjP"
                     /locus_tag="ACHJ48_000660"
     CDS             34198..35040
                     /gene="ycjP"
                     /locus_tag="ACHJ48_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO4706279.1"
                     /translation="MATNKRTLSRIGFYRGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            35071..36123
                     /gene="ycjQ"
                     /locus_tag="ACHJ48_000661"
     CDS             35071..36123
                     /gene="ycjQ"
                     /locus_tag="ACHJ48_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO4706280.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETIIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWKLLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            36141..36929
                     /gene="ycjR"
                     /locus_tag="ACHJ48_000662"
     CDS             36141..36929
                     /gene="ycjR"
                     /locus_tag="ACHJ48_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO4706281.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KSAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYSVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            36939..37994
                     /gene="ycjS"
                     /locus_tag="ACHJ48_000663"
     CDS             36939..37994
                     /gene="ycjS"
                     /locus_tag="ACHJ48_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4706282.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGYPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLGSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            37991..40258
                     /gene="ycjT"
                     /locus_tag="ACHJ48_000664"
     CDS             37991..40258
                     /gene="ycjT"
                     /locus_tag="ACHJ48_000664"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO4706283.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEICAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRSGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            40255..40914
                     /gene="pgmB"
                     /locus_tag="ACHJ48_000665"
     CDS             40255..40914
                     /gene="pgmB"
                     /locus_tag="ACHJ48_000665"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO4706284.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            40928..42010
                     /locus_tag="ACHJ48_000666"
     CDS             40928..42010
                     /locus_tag="ACHJ48_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4706285.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGMRPEDIHPDAQEENNISAKISVVELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            42055..42960
                     /gene="ompG"
                     /locus_tag="ACHJ48_000667"
     CDS             42055..42960
                     /gene="ompG"
                     /locus_tag="ACHJ48_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO4706286.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSISLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(43071..44069)
                     /gene="ycjW"
                     /locus_tag="ACHJ48_000668"
     CDS             complement(43071..44069)
                     /gene="ycjW"
                     /locus_tag="ACHJ48_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4706287.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHTPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLEDHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGRQSCELLFRLIAGKPSPQNITVATHMTLK"
     gene            44224..45621
                     /gene="ycjX"
                     /locus_tag="ACHJ48_000669"
     CDS             44224..45621
                     /gene="ycjX"
                     /locus_tag="ACHJ48_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO4706288.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            45618..46679
                     /gene="ycjF"
                     /locus_tag="ACHJ48_000670"
     CDS             45618..46679
                     /gene="ycjF"
                     /locus_tag="ACHJ48_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO4706289.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            46827..48368
                     /gene="tyrR"
                     /locus_tag="ACHJ48_000671"
     CDS             46827..48368
                     /gene="tyrR"
                     /locus_tag="ACHJ48_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO4706290.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(48412..48918)
                     /gene="tpx"
                     /locus_tag="ACHJ48_000672"
     CDS             complement(48412..48918)
                     /gene="tpx"
                     /locus_tag="ACHJ48_000672"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO4706291.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            49037..50002
                     /gene="ycjG"
                     /locus_tag="ACHJ48_000673"
     CDS             49037..50002
                     /gene="ycjG"
                     /locus_tag="ACHJ48_000673"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO4706292.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDTTLIVDANESWHAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(49977..50705)
                     /gene="mpaA"
                     /locus_tag="ACHJ48_000674"
     CDS             complement(49977..50705)
                     /gene="mpaA"
                     /locus_tag="ACHJ48_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO4706293.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(50996..51634)
                     /locus_tag="ACHJ48_000675"
     CDS             complement(50996..51634)
                     /locus_tag="ACHJ48_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4706294.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSPKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(51655..52585)
                     /locus_tag="ACHJ48_000676"
                     /pseudo
     CDS             complement(51655..52585)
                     /locus_tag="ACHJ48_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(52585..53505)
                     /locus_tag="ACHJ48_000677"
     CDS             complement(52585..53505)
                     /locus_tag="ACHJ48_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4706295.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            53643..54542
                     /gene="pgrR"
                     /locus_tag="ACHJ48_000678"
     CDS             53643..54542
                     /gene="pgrR"
                     /locus_tag="ACHJ48_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO4706296.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLIIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            54878..56491
                     /gene="mppA"
                     /locus_tag="ACHJ48_000679"
     CDS             54878..56491
                     /gene="mppA"
                     /locus_tag="ACHJ48_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO4706297.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDVHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(56542..57573)
                     /gene="ynaI"
                     /locus_tag="ACHJ48_000680"
     CDS             complement(56542..57573)
                     /gene="ynaI"
                     /locus_tag="ACHJ48_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO4706298.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            57817..58074
                     /gene="ynaJ"
                     /locus_tag="ACHJ48_000681"
     CDS             57817..58074
                     /gene="ynaJ"
                     /locus_tag="ACHJ48_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO4706299.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(58124..59074)
                     /gene="uspE"
                     /locus_tag="ACHJ48_000682"
     CDS             complement(58124..59074)
                     /gene="uspE"
                     /locus_tag="ACHJ48_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO4706300.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(59226..59978)
                     /gene="fnr"
                     /locus_tag="ACHJ48_000683"
     CDS             complement(59226..59978)
                     /gene="fnr"
                     /locus_tag="ACHJ48_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO4706301.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(60173..60688)
                     /gene="ogt"
                     /locus_tag="ACHJ48_000684"
     CDS             complement(60173..60688)
                     /gene="ogt"
                     /locus_tag="ACHJ48_000684"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO4706302.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGELAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(60699..62223)
                     /gene="abgT"
                     /locus_tag="ACHJ48_000685"
                     /pseudo
     CDS             complement(60699..62223)
                     /gene="abgT"
                     /locus_tag="ACHJ48_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            complement(62260..63705)
                     /gene="abgB"
                     /locus_tag="ACHJ48_000686"
     CDS             complement(62260..63705)
                     /gene="abgB"
                     /locus_tag="ACHJ48_000686"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO4706303.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAIKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(63705..65015)
                     /gene="abgA"
                     /locus_tag="ACHJ48_000687"
     CDS             complement(63705..65015)
                     /gene="abgA"
                     /locus_tag="ACHJ48_000687"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO4706304.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNENSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTTIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGYNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            65191..66099
                     /gene="abgR"
                     /locus_tag="ACHJ48_000688"
     CDS             65191..66099
                     /gene="abgR"
                     /locus_tag="ACHJ48_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4706305.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            66429..66992
                     /gene="smrA"
                     /locus_tag="ACHJ48_000689"
     CDS             66429..66992
                     /gene="smrA"
                     /locus_tag="ACHJ48_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO4706306.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(67013..68245)
                     /gene="dgcM"
                     /locus_tag="ACHJ48_000690"
     CDS             complement(67013..68245)
                     /gene="dgcM"
                     /locus_tag="ACHJ48_000690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO4706307.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            68439..68486
                     /gene="ydaN"
                     /locus_tag="ACHJ48_000691"
     CDS             68439..68486
                     /gene="ydaN"
                     /locus_tag="ACHJ48_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO4706308.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            68500..69483
                     /gene="zntB"
                     /locus_tag="ACHJ48_000692"
     CDS             68500..69483
                     /gene="zntB"
                     /locus_tag="ACHJ48_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO4706309.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            69758..69928
                     /gene="ynaL"
                     /locus_tag="ACHJ48_000693"
     CDS             69758..69928
                     /gene="ynaL"
                     /locus_tag="ACHJ48_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO4706310.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            69961..71334
                     /gene="dbpA"
                     /locus_tag="ACHJ48_000694"
     CDS             69961..71334
                     /gene="dbpA"
                     /locus_tag="ACHJ48_000694"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO4706311.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(71463..72398)
                     /gene="ttcA"
                     /locus_tag="ACHJ48_000695"
     CDS             complement(71463..72398)
                     /gene="ttcA"
                     /locus_tag="ACHJ48_000695"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO4706312.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(72574..73008)
                     /gene="uspF"
                     /locus_tag="ACHJ48_000696"
     CDS             complement(72574..73008)
                     /gene="uspF"
                     /locus_tag="ACHJ48_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO4706313.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(73149..74262)
                     /gene="ompN"
                     /locus_tag="ACHJ48_000697"
                     /pseudo
     CDS             complement(73149..74262)
                     /gene="ompN"
                     /locus_tag="ACHJ48_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
BASE COUNT        18447 a        18300 c        19471 g        18172 t
ORIGIN      
        1 gctgcgggtt tccatgcgga cttcggcaat ggtgacgtca ataacgtcag ttactttata
       61 aaccgtttca cctttaattt gtacggtgcc gttttcctgg ctgcaaacca gttcatcgcg
      121 cacagcgtgt aagaaaggtg caggaataaa ggcgatagcg ccgttatcaa ccaaacgaac
      181 acgcatgccg ccacggctga tatcgacaat ttccgctgcg aaacgggtgt cggtcccggc
      241 tttgtctttc aggaagcgtg cgtataacca gtcaccaaca tcacgttctg ccatccgatt
      301 gagacggcga cgctcggcca tttggacagt gatttcatcc tgtggacgcg tcgcagtttc
      361 gcctttgata accgctttca gcagacggtg gttgatcatg tcgccatatt tacggatcgg
      421 cgaagtccag gtggcgtatg cttccagacc gaggccaaag tgaggaccgg gttcagtgct
      481 aatttcagca aatgactgga agcgacgaat gcggctgtcg aggaaaccag ttggttgcgc
      541 atccagttca cgacgcagtt tgcagaaacc gtccagcgtg agcacttctt cggcatcgac
      601 atgcagaccg tgcgttttca gcaacgctgc cagcgcgtcg gcattcgccg gatcaaagcc
      661 catatgcacg ttatagatac caaaaccgag cttatcgcgc agtacgcgag ctgcacaaat
      721 gttagcggca atcatcgctt cttcgacgat acggttggca atgcgacgag gctcggcgac
      781 gatatccagc acttcacctt tttcaccgag aataaagcgg taatccgggc gatctttaaa
      841 caccagtgcg tggttatgac gccactcgcc gcggcgttgg caaatttgcg ctagcaaacg
      901 gacttgttcg gcaattgctt cactttcagg ctgccagtca ccggtattct ccagccagtc
      961 agaaacctgg tcatacacca gcttcgcttt ggattcgatg gtggcggcaa agaattcgat
     1021 attatcttca atggtgccat cagcggagag cgtcatgcgg catgccagta ccgggcggac
     1081 ttcattggcg cgcagtgagc agagatcgtc agaaagctcg cgaggcagca tagggatgtt
     1141 gaagccaggc agatagttgg tgaatgcgcg aattttcgcg gctttgtcca gcttgctgcc
     1201 ttcagcaatc cacgcggttg gatcggcaat cgccacaatc agctgaagtt tgtcatccgg
     1261 caacgcctta gcgaaaaggg cgtcatccat atcttctgtg ctggcactgt cgatggtgac
     1321 aaaatccagc gcggtcagat ctttacgaac cagaccttca tcgagcattt cggtagcgac
     1381 gccgtctggt gcttcttttt ccagattatg gcgcgcaagg gtaacccacc acggtacaaa
     1441 gtggtcgtca ccaaaagtga tgtattgtgt cagttcagca tagaaagaac gatcgccttt
     1501 cagcggatga cggcgcattt cggcaactgc ccagtcgcct tctttaaact cgtggttcag
     1561 accacgggct gcgcggcaag gaatggcgtc ttttaagagt ggatgatcag gaacgatggc
     1621 cagacggtca tttttgccct gaactttacc cacgaaacga gtcagaaacg gttcaaccag
     1681 ttcttctggc tctgcggatt cacgttcttt ttcactgtgg atcaccgcga taattcggtc
     1741 gccatgcatg acttttttca tctgcggcgg cggaatgaaa taactttttt gcgcgtcgac
     1801 ttccaggaag ccaaaacctt tttctgtggc ttttaccacc ccttcagcgc gtggcgtctg
     1861 ggaatgcagt tgctgtttaa gctgcgctag cagcgggttg tcctgaaaca taattgtcta
     1921 ttttggtggc cattagagcg gctgacagtt ttacgcgaat ctgtctgacg cggcaaggtt
     1981 aatatgtctc acccaacgcg atttttaagc gatttatcca gccacacagc ccgctccata
     2041 ccagcagatt aataatctgc gttgatgatt tttcgtgttc gagtaaaggg ctgaactggg
     2101 cggcgctaaa gcgatcgggt gctcgggtca gccattgtat cgcctgaacg gtagcgcgct
     2161 caacagaatg acggtcagcc cattggttaa tttcattttc gcccttgcgc agaagggggc
     2221 tgattttttt atattcattt gatgaatcca tacaggcggc aaaacaagca ggactgccat
     2281 ttatccgcgc gcataacaac gccgcaagct gattatccgg ctgtgtatta acccgtgtgc
     2341 tgtttaacaa cctttcgaga agggaaagtg aaatagggtg gagagagagt atttcggcca
     2401 gttggcaaag ttctgcagtt gaactttttg tgtgttcttc aggtagtttt tccacctcat
     2461 agctgcttcg ccagcgtaat gattcatcag caaacagtga cgcgtcggcg tagttttgta
     2521 tctccagccc gggtaaccag cgtaccggat gcccgagata agcctgaaat gtcgcaattg
     2581 tccgcgcctg aaagccaata aaaccaatga tttggttaat gatcacacag tcccaggcag
     2641 taagaccgac gtcattcaga tgctggcgcg atcggttgtc gataatagaa ggcgagctag
     2701 ccagttggcg ggcatattgc gtgatttgtg ccaaccgatg attactttcc ctggtggaat
     2761 caggcccggg gagcggcgta agtcgcgccg aatagtagtt acataaccgc tggacgccgc
     2821 aaacctgagc aaccgtcagg gccgtcgaca agcgttcata agcggtgaag gtctgtgaac
     2881 gggtcacggt aagctggtca gggaacaact ccactgcaag aacacgcgca gggtccagcc
     2941 atgaaaggaa gggggccagc gtttcttttt caaggatcac gtcgagtaga aagggatcgc
     3001 tgacctttgc ggcttccggg accagaggca gaacgtcata ctccgtagta ctggattgcg
     3061 tttcatgata ccagtggctt ttgccggtga tgtggcgttg ttccatgggc gctccttggt
     3121 cgtaaaggaa atcgttatcc tgacgcaagg cgggaagggg agaaagacgg atcggcgata
     3181 acaaatatca gaaaggtata acagagataa cgggcggcag aacgccgccc atctttacca
     3241 acagaacgat tatttcagtt cgagttcgtt cattgcagca atgctgaaac cgccgtcaac
     3301 gtggaccact tcaccggaga taccggcaga gagatcggag cacaggaatg ccgcagagtt
     3361 acccacatct tcaatagtaa cggtacggcg aatcggggta acggcttcgc aatgagccag
     3421 cattttgcgg aagtctttga taccggaggc cgccagagta cggatcggac cagcagagat
     3481 ggcgttaaca cgcacacctt ccggacccat cgcgttcgcc atatagcgca cgttcgcttc
     3541 cagagacgct tttgccagac ccataacgtt gtagttcggg atagcgcgct cagcgccaag
     3601 gtaggaaagg gtcagcaggg cagaacccgg attcagcatg gagcggcaag cttttgccat
     3661 tgcaacgaag ctgtaggagc tgatgtcgtg ggcaattttg aagccttcac gagtaacggc
     3721 gttaacatag tcaccatcca gctgatcgcc aggtgcaaaa ccaatagagt gtacgaaacc
     3781 gtcaaatttc ggccaaactt tccccagttc agcgaacatg gtgtcgatgc tggcatcttc
     3841 ggcaacatcg cattgcagaa cgatgtcaga acccaattga gcggcaaatt cttctacgcg
     3901 gcctttcagt ttgtcgttct ggtaggtgaa tgccagttca gctccttcgc ggtgcatcgc
     3961 ctgagcgata ccgtaggcga tggacagttt gctggcaaca ccggttacca gaatgcgctt
     4021 accggaaaga aaacccatag ctttaatcct tattgttgat gcttgttgtg cctgaaaatc
     4081 aggcgattcg tcgttttagt aaacagtacg aacagataaa cggttattat aatcaacctg
     4141 gctgtgggtg gctatagttg cgctttctgt accggagaga agctgcggta cagagcattt
     4201 tgcgttatct actcttgacg cctgcaaggg gtaggttggg gaaatatctt atagaatctg
     4261 actatgaaac tattcgtatt agctccgata agtttgatga tgaaaacaga actggcgaca
     4321 acaagtagca gcctttctga tgaattgacc ccaacttaat attgtcaact atcttcacag
     4381 aatctccaca atctcttcat tatcagaata tgcaccatga ataagataaa aatactttcc
     4441 attactttgg agaaacgtat aatcagcaaa aatgagaacg atcattctca ccaagtgata
     4501 ggccaggaga cagaaatgac cagcaagctg gaaatacgcc acaaacagcg tcaggatgaa
     4561 atcattaacg ccgcccgtcg gtgttttcgt cgctgcgggt ttcatgccgc cagtatgtcg
     4621 caaattgcca gcgaagctca gcttagcgtg ggtcagatat atcgttactt cgccaacaaa
     4681 gacgccatta ttgaagagat ggttcgccgt atcatcgact ttcgtattgc ccaaatggac
     4741 attgacgcgc gtaccgatca cttcccggaa gtccttgcct tgcgtaaatc cttaaatgag
     4801 gacgacgacg cgctgatgct ggaagtggct gcagaagcca cacgcaaccc gcgggttatg
     4861 gcaatgcttg aagaagctga cgccagaatg ttcgctaacg gttgtgcaca tatgaaaaga
     4921 atgcatcccc atcttagcga ggagcacatt cgttgctgcg ttgaagtgtt tgctaccatg
     4981 atggaaggta cggtttatcg tcgtttaaca ccgcaaaaaa gcgatccaca acatctgcag
     5041 gaaatatatc aggatattgt tagtatgttg ataaataaat aagaacagaa ttaaccaata
     5101 gcgggggata ttaatccccc ttatttcatt gcgaaattca tttcgcgatg cgggttcgtt
     5161 ataactgaat atccctgaca tatcctcatt aattttaatg aggagggtat ttctcaccct
     5221 ggagaaaata atgaagtata tagcaacatc tgtagtggca atgctgctct tatctggttg
     5281 tgataatacg caaagtaaca attcatcccc gtcagaaaca gaagtcggcg ttgttacagt
     5341 caaatctcaa ccggtctcgg tagtcagtga attaaccgga cgcaccagtg ctgcgctcag
     5401 tgccgaagta cgtccgcagg ttgggggaat tatccagaaa cgcttattta aggaaggtga
     5461 tctggtcaag gctggacagc cgctctacca gattgatgcg gccagttatc aggctgcatg
     5521 gaatgaagct cgggcagcat tacaacaagc acaggcactg gtaaaagccg attgccagaa
     5581 agcgcagcgt tatgcccgac tggtgaaaga gaacggtgtt tcacaacagg atgctgatga
     5641 tgctcagtct acctgtgcac aagataaagc cagtgtagcg gcgaaaaaag ccgcactgga
     5701 aactgcgcgc attaatcttg actggaccac ggtaaccgca ccgatttcag ggcgcattgg
     5761 catttcgtcg gtaacccctg gcgcactggt gaccgcgtcg caagatacag cgttaacgac
     5821 tattcgtggt ctggatacaa tgtatgtcga cctcactcgc tccagtgtcg atttattacg
     5881 tctgcgtaaa cagtcactgg cgaccaacag tgacaccatg agcgtctcac ttattctgga
     5941 agatggcaca acctacagcg aaaaagggcg tctggaactc accgaagtcg ctgtagatga
     6001 gtctaccggt tcggtgacat tacgggcgat tttccccaat ccacaacagc aattattacc
     6061 gggaatgttt gttcgcgctc gtgtcgatga aggcgtgatg gaagacgcta ttctcgcgcc
     6121 gcaacagggc gtcacgcgcg atgctaaagg caatgcaact gcgctggtgg tgaataaaga
     6181 caataaagta gagcagcgaa cgctcgaaac gggagaaaca tatggtgata aatggctggt
     6241 gctaaacggc ctgcacagcg gcgaccgact gattgttgaa ggttctgcca aagtcacttc
     6301 agggcagacg gtcaaggctg ttgaagttca ggctaatgga ggcaacgcct gatgttttcg
     6361 cgcttttttg ttcgccgccc ggtctttgcc tgggttatcg ccattttgat tatgttggcg
     6421 gggattctgg ctattcgcac attgcccgtc gcgcaatatc ctgacgttgc gccaccgacc
     6481 attaaaattt cagccactta tactggtgct tctgccgaaa cgctggagaa cagcgtgact
     6541 caggtcatcg agcaacaact cactgggctt gataatttac tctatttcag ctcaaccagc
     6601 agctctgatg gttcggtcag tattaatgtg acctttgaac aaggtaccga tccagatact
     6661 gcacaggtgc agaataaaat tcagcaggcg gagtcgcgcc tacccagcga agtgcagcaa
     6721 acgggtgtta cggtggagaa atcacaaagc aactttttgc tgattgctgc cgtgtatgac
     6781 accactgaca aagcctccag ttcggatatc gccgactggt tggttagtaa cgttcaggac
     6841 ccgctggcgc gtgttgaagg tgtggggagt ctgcaagtct ttggcgcgga atacgctatg
     6901 cgcatctggc ttgacccggc caaactggcg tcttactcgc taatgccttc agacgtgcaa
     6961 agtgctattg aagcgcaaaa cgtgcaggtt actgccggga aaataggggc attgccttca
     7021 ccgaatactc agcaactgac cgcaacggtt cgtgcgcagt ctcgcttgca gacggtggat
     7081 cagttcaaaa atattatcgt gaaaagccag tcagacagcg cagttgttcg tataaaagat
     7141 gtggctcgcg ttgagatggg cagtgaagat tataccgcta tcggcaaact taacggtcac
     7201 ccgtctgccg gggttgctgt aatgctttcg cccggtgcga atgcgctgaa tacggcgacg
     7261 ctggtcaagg ataagattgc cgaattccag cggaacatgc cgcagggata cgacatcgct
     7321 tacccgaaag acagcactga atttattaaa atctctgtag aggacgtaat tcagacgctg
     7381 tttgaagcca tcgtattggt ggtttgcgtg atgtatttat tcctgcaaaa cctgcgtgcc
     7441 acactgattc cggcgttagc tgtccccgtc gttttgctgg gcacattcgg cgttcttgcg
     7501 ttgtttggct attcgattaa taccctgacg ttgtttgcta tggtgctggc gatcggcttg
     7561 ctggtggacg atgccattgt ggtagtggag aacgtcgaac gcattatgcg tgacaaaggg
     7621 ttgcccgcgc gtgaagccac ggaaaaatca atgggcgaga tttctggcgc actggttgcc
     7681 attgcgctgg tgttgtcagc cgtattcctg cctatggcct tctttggggg atctacgggg
     7741 gtaatttatc gtcagttttc gataaccatt atctccgcaa tgctgctttc cgtggtggtg
     7801 gcattgacct tgactcccgc cctgtgcggt tccgtcctcc agcatgttcc gccacataaa
     7861 aaaggctttt tcggcgcatt tgaccgcttt taccgccgta ctgaagataa atatcagcga
     7921 ggcgtaattt atgtcctgcg ccgtgctgct cgaacgatgg ggctttatct cgtactgggt
     7981 ggtggaatgg ccctgatgat gtggaaactg ccgggcagtt tcttacccac tgaagaccag
     8041 ggcgaaatca tggtgcagta cacgctgccg gccggggcga ccgctgcccg tacagcagaa
     8101 gtgaatcgcc agattgttga ctggttcctg attaacgaga aagcaaatac cgatgtcatt
     8161 tttaccgttg atggtttcag ttttagcggc agcggacaga acaccgggat ggcgtttgtt
     8221 tcgttgaaaa actggtctca acgtaaaggg gcagaaaaca ccgcccaggc tatcgcccta
     8281 cgggcaacca aagagctggg cacaattcgt gatgccacgg tatttgcgat gacgccgcca
     8341 gccgttgatg ggctggggca aagcaatggt tttacgtttg aattgttagc taacggtgga
     8401 accgatcgcg aaacactact gcaaatgcgt aatcagttga tagaaaaagc gaatcaaagt
     8461 ccggagttac attctgttcg tgccaatgat ttgccacaaa tgccgcaatt gcaggtagat
     8521 attgatagta ataaagcggt gtcattaggg ttgagtttga atgatgtcac cgacaccctg
     8581 tccagtgcgt ggggcggtac ttatgtgaat gactttattg accgtggtcg tgtgaaaaaa
     8641 gtctacattc agggcgacag tgaatttcgt tccgcgccgt cagacttagg taaatggttt
     8701 gtgcgcggta gtgataacgc catgacacca ttctctgctt ttgcgaccac ccgctggctg
     8761 tatggaccgg aaagactggt gcgctacaac ggttctgcag cctatgaaat tcagggggaa
     8821 aatgcgactg gctttagttc cggcgatgca atgacaaaaa tggaggaact ggcaaacagt
     8881 cttcctgcgg gaacaacctg ggcctggagt ggtttgtcat tgcaggagaa actggccagc
     8941 ggtcaggcat tgagtctgta tgcagtttct attctggtgg ttttcctctg ccttgcagca
     9001 ctgtacgaaa gctggtcagt cccgttctcg gtcattctgg tgatccctct ggggcttctt
     9061 ggcgcggcgc tggcggcctg gatgcgtgat ttaaacaacg atgtttactt ccaggtggcg
     9121 ctattaacca ctatcggctt gtcgtcgaaa aacgccattc tgattgtgga atttgctgaa
     9181 gcggcggttg cagagggtta ttccttgagt cgtgcggcat tacgcgcggc gcagactcgt
     9241 ttacgcccaa tcatcatgac ctcgctggcg tttattgcgg ggggtaatgc cgctggcgat
     9301 agcaaccggc tcaggggcga acagccgcat cgccattggt acgggcatta ttggcggtac
     9361 gctgaccgct acgttgctgg ctattttctt tgttcctctg ttttttgtac tggtgaagcg
     9421 tttgtttgcc ggtaaatcgc gccgtcagga gtaagttatg ttgcgtcgtt ctcttatttt
     9481 tctggtgctg ttgagtgcgg gatgtgtctc gctcgatccc cactacagca cgcctgaatc
     9541 cccaatcccg gcgaccttgc cgggagcaca gggccaggga aaggcaatca gccatgactg
     9601 gcagcaggtg atccacgatc cccgattgca gcaggtggtg actattgcac tgaacagtaa
     9661 ccgcgatgtg caaaaagcga ttgctgatat tgactcagca cgggcgctct atgggcaaac
     9721 taatgcgtcg ttattcccga cggtgaatgc ggccttaagt tcaacccgca gtcgttcgct
     9781 ggcgaatggt acggaaacca ctgccgaggc tgacggcacg gtgtccagtt ttacgttaga
     9841 tctgtttggt cgtaatcaga gtttatctcg ggcggcgcga gaaacctggc ttgccagtga
     9901 atttacagcc cagaacaccc gtttaacgct gattgctgaa atcagcaccg cctggctgac
     9961 actggcggcc gataacagca acctggccct ggcaaaagaa acgatgacca gtgcagaaaa
    10021 ctcattgaaa attatccagc gccagcaaca ggttggcacg gcggcagcga cagatgtcag
    10081 tgaggcgatg agtgtttatc agcaagcgcg cgccagcgtt gccagctatc agacacaagt
    10141 gatgcaggat aaaaatgcgc tgaacttact ggcaggtaca acgcttgagg aaaatttgct
    10201 accgggaaca ctggaaagcc tgccggaaca gatgataagt ctggttcctg cgggagtgtc
    10261 atctgacgtt ctgctgcgtc gcccggatat ccaggaagcg gaacataatt tgaagagcgc
    10321 caatgccgat attggcgcgg cgcgggctaa ctttttcccg acgatttcgc tgactgccag
    10381 cgctggtgtt ggcagtgacg cattgtcgtc gttgttcagc cacggaatgc agatctggtc
    10441 atttacgcca tcggtcacat taccgctgtt cactggcgga agtaatctgg cgcagcttcg
    10501 ttacgcagaa gcacaaaagc gtggactcat tgccacctat gaaaaaaccg ttcagagagc
    10561 atttaaagat gtcgctaacg cactggcgcg gcggaccacg cttgaagagc aactggatgc
    10621 acagcgccag tatgttaaag ctgagcaaca aacggtcgat gttggcttgc gtcgctatca
    10681 gacgggtgtt ggtgattacc tgaccgtgtt gacggcgcag cgcagcttgt ggagcgcaca
    10741 gcaggagctg ctggcactgc aactgactga ttttacaaac cgaatcacgc tctggcagtc
    10801 gctgggcggc ggcatgtcat cactcaagta aggaataata tggctagagt ctctctttca
    10861 tgggcattga ttcttggtct tttagccggt atcggcccga tgtgtaccga tctttatttg
    10921 ccggctttgc cggagatgtc tgagcaactg gcggcaacca cgaccataac gcaattaacg
    10981 ctgactgcat cactgatcgg tcttggcgtc ggacaactgt tatttggccc tctgagcgac
    11041 aaaatagggc gtaaacgtcc gttaatcttg tcgttgctat tgtttattgt ttcttccatt
    11101 ttgtgcgcga caacgaacaa tatttactgg ctggtggtct ggcgttttat tcaagggatc
    11161 gcgggggcgg gtggttcggt gctctctcgt tctattgctc gtgacaaata tcagggagta
    11221 acgttgaccc agttttttgc gctgttaatg acggtgaatg gcctggcacc ggtgttgtcg
    11281 ccagtgctgg gcgggtacat tgttagcact tttgactggc gcactttatt ctgggtaatg
    11341 gctgaaatta gcaccgtatt gttgctgggc tgcgtgttat ttattaatga gaccttgcca
    11401 gaaaataaaa ggggctcatc attgctatta accggacgaa gcgtggtgca gaaccgccgt
    11461 tttatgcgct tttgcctgat tcaaagtttt atgctggcag gcttgtttgc gtatatcggc
    11521 tcttcgtcgt tcgtgttgca gaaggaattt ggctttagtc caatgcaatt tagcctggtg
    11581 tttggcctta acggcatcgg acttatcatt gcttcatgga tcttttcgcg tctggcgcga
    11641 cggattaatg cgatgacatt gttgcgaggt ggcctgatag cggcaatttt gtgtgcattg
    11701 ctcacggtct tatgcgcatg gacacaattg cccattccgg cactggtggc attatttttc
    11761 actattgcat tttgtagcgg catcggcact gttggcgggg cagaggctat gagtgcagta
    11821 gggacgcagg aatctggaac ggcgtctgcg ttgatgggga tgagcatgtt tgtcttcggc
    11881 ggtatagccg cgccattgtc gggatttggc ggagaaacac tgttaaaaat gagtctggca
    11941 attacggtgt gttatacact ggcattcctg gttgctctca ccagaatcga caatcaaaag
    12001 taatgaatac ctcatcctga ggggggttcc cctgtcagga tgaggtgcag aacactaacg
    12061 gtccttacgc cacgcatccg ccgtcaatgc ttcgccaaag tgcccggcaa tcagccgttt
    12121 ggtgagttca tgcagcggcg atgccagcac atccgcggtg ctgcctcgct cgacaacctc
    12181 gccctgatgc atcaccagca cctggtcgct aatgtgcttc atcattccga tatgctgggt
    12241 aacataaata tacgaaatgc cctgtttttc ctgtaattcc agcatcagat taatcaactg
    12301 cgaacgcatc gacatatcca gtgaagcgag ggcttcatcg gcaataatga cttttgggcg
    12361 caatatcagc gcgcgcgcca gacccagacg ctgtttttgc ccgggggcca acatatgcgg
    12421 atagtaactg acgtgatccg gtagcagccc aaccatacgc atcgtttcaa taatctgttt
    12481 gcgacgctgt tccggttcca ggttggtgtt caggcgcagt ggaaaatcca gaatttgcga
    12541 gatacgttga cggggattca acgaggtcga aggatcctga aaaatcatgc gaatacgctg
    12601 actgcggaag gaataatcgc caaaatgcag tggatgatcg tcaatcaata actcgccgct
    12661 ggtaggttct atcattcccg ccagcatttt tgccagcgtg gatttacccg aaccattctc
    12721 gccaataatc gccagtgtct ggccttcacg tagcgtaaag ctcaagggtt ttaccgcttc
    12781 tacggtctga cgacgaaacc aaccggtccg gtagcggaac gttttactta aattacgcac
    12841 ttcaagcagc gtttcgatca tctcactctt tctccatgtt cagcgggaaa tgacaggcat
    12901 agagatgatt tttcgccccc gtcaaacgtg gcgtcacaat gcattctcgt tgtgcatacg
    12961 ggcaacgtgg ccccagacga caaccaatcg gtaactgttc aagcagtggg atagcgccgg
    13021 gtagcgtatt gaggcgactt ttatgcggca tcgcgcttcc gaagtctggt atcgcgcgga
    13081 tcagcgcctg ggtataagga tgatgtggca tcgtcactaa ctccttactc ggcgcggttt
    13141 ccaccgtttg accgcagtaa agcacgttaa ttttatccgc ccattggcta agcatttgta
    13201 agtcatggct gataagcaaa atagtggtat tgctgttttg gtttagacgc gtcagcaggc
    13261 gaaagatttg cgcctgggtt gttggctcca ttgagtttgt cggttcgtca gcaatcagca
    13321 gacgcggttg attcgccagt gcaatggcta tcatcacttt ctgacattca ccttcggtca
    13381 actcataggg aaaactgcgc atcgcatctt tatgatcttt aattcccacg cggtgcagca
    13441 gttcaatggc acggcgtttg cgccagccaa aacgctgcca ccaacggcct ttataggtcc
    13501 aggctgggat gttttgcatc aactggcgac ccacacgttc tgaaggatct agacacgact
    13561 gcggttcctg gaaaatcatc gacacgttgt ggccaaccag tttgcgccgt tcgcgtgcgg
    13621 agagacgcag caaatcgata tcatcaaaac gcatacggtc tgcagtaaca cgccagttat
    13681 ctttattcac cccacaaatc gctttcgcaa tcaaactttt gccggaaccg gattcaccaa
    13741 caagaccgcg gatttcacct tcggttaagg tcatacttac ccggtcaacg gctttaaccc
    13801 actcatcacc ggttttaaat tcaatagtca ggttacgaat atcgagtaat ggcattattc
    13861 cacccccgca ataattgcac gacggacacc gtcgccaagg aggttaacca acagcacgct
    13921 aatcataatt gccgcacctg gcagcatgac agtccacggg gcgacataaa tcagttccag
    13981 cgcatcaccg agcatcgctc cccattcagg cgaggggagt tgtgcgccaa gatcgagaaa
    14041 gcccagcgcg gcgatatcga gaattgccat cgacagtgcg cgggtgatct cggtgaccag
    14101 cccggcggtg atgttaggca tcacagcaaa ccagagaata ttcagcgttg atgcgccatc
    14161 cagacgggcg gcgataacgt actctttttc cagttcgtca tgcaccatgc tgtaaatcga
    14221 acgtaccata cgcggcagca gcgccagcca gacagcaaac atagcgtggg acaaactcgg
    14281 tccggcaaac gctacaacga taattgccaa cagcagggaa gggatcgcca gtaaggtatc
    14341 cagaatatgg ttaagcaccg ctgagcggag gccgtgcgtc gccccggcga aggtgcccag
    14401 taccaggcca cagatcgtcg cggcaagtgt caccacaaat gcgccaccca cggttggcgc
    14461 agctccgctc agtaaacggc ttaaaacatc gcgtccgagg tcgtcagtcc ccaggaagaa
    14521 agaaacttcg ccatagcgcg accatgatgg cggcaataat tggtaaccga gaaattgctg
    14581 gtcgatgccg taaggcgcaa accagccgcc aaacatacac agtacagcca gccccgcgca
    14641 gccgtacagg ccgaccatcg cagaggcgtc actataaaat ttgcgccagg cggtacgcag
    14701 cgtgcccggc gggcgctttt cgctgtatac gctatcgtaa ggcataccat tccttatgtt
    14761 tcagagggtt agccatggca cccaaaatat cagaaatcac gttaacaata atgaccagtg
    14821 agccacacac catcactccg gcggaaatgg ctgcgtaatc ctgctggcgg atggcgttaa
    14881 ttaaccagcg ccctaaaccc ggccagctaa aaaccatttc ggtgatcatc gccagcgtca
    14941 gcatggtgga aaactgtaag cccaggcgag gaataaccgg cggtaacgcg ttatgcagaa
    15001 cgtggcgacg caaaatagta aagcgcgaca aaccgcgggt cgccgccgct tttacatagt
    15061 tctggtcata cacttcgata gtgctgatac gcatcaggcg gatcacttca gttgttggcg
    15121 caaccgacag ggtgatcacg ggcaatatca tatggcggat tgcgctcatg atcatttcat
    15181 cccgccatgg tgagtccgaa agccaggcat caatcaacgc aaaaccggta atcggtttca
    15241 cttcgtagag cagatcgaaa cgccctgaaa cgggcaacca acctagcgtg agtgaacaaa
    15301 acagtgtcaa cagaagcgcc agccagaaca ccgggattga aaagcccagt aaggcgatgg
    15361 cgttgatcaa gttatcctgc cacttatggc gcgtaatccc ggcaatcatc cccacgggga
    15421 tgccaacaat cagcgcaaaa ccgaaggcga ggatgcacag ttccatcgtc gccgggaaga
    15481 cctcttttaa ctgttcggca attggttgac cgttaatgct ggaaacacca aaatcccagt
    15541 ggatcaggcc gttaaaccag aacacccagg cattccacag tgacgcgcct tgtaacggcg
    15601 cgtgtggagt gaaatagctc aagctgaagc caacgaaggt cagcaggaac aaggtgacaa
    15661 tcaataacaa aatgcggcgt aaggtgaaga taatcatggt tttttcacct catcctgttt
    15721 ctcgcgatac accccagcaa aggaggcgtt accaaacggg ctaagtacca gacctttgat
    15781 atcgtaccga taggcctgca aacgcaatga cgacgccagc ggcaaaatgg gcaattcctg
    15841 cgccagaata ctctgcgctt cgtcataggc ttcaatacgc gccgccagct gctgcgagga
    15901 gagcgtctta cgcaacacgc tgtcgaattt cggatcgcac cagtgggcga ggttggtctg
    15961 tgaatgaatt gccgcgcagc tcagtaacgg acggaagaaa ctgtccgggt cgttactgtc
    16021 cgtcgcccaa ccggataacg tcagatcatg gctcatatcc atcaaccgcg cctcctgaaa
    16081 gcgaccttct accggcacaa tcaccacttt tacgccaacc tgcgccatat ccgcctgaat
    16141 cagttcggca gttttcagtg gactggggtt ccacgcctgc gaacgtgtgg gcacccacag
    16201 tttcagcgtt aaattttcca gccccaacaa cttcaactgt tcgcgcgatt tcgccggatt
    16261 gtattcagta attttagcct cgttgtcata ggcccacgag gcgcgcggta aaatagaggc
    16321 cgccgtttca gccgtaccat aatagatgga ttgcatcagg cgctggttat taatcgccag
    16381 tgccagcgca tggcggacag cgggattatt tagcggcggt ttggcggtgt taaatgccag
    16441 ataggcgacg ttcatcccag gacgcagcgt taaacgcaag cgcgggtcgt cacgcaaaat
    16501 ggatagctgg ctggcagcag gccaggccag aacgtcgcat tccccggtca ggagtttcga
    16561 cagacgtccg gtgccgccgg agcctaaatc caccaccacc tgcggcatta acggtttacc
    16621 gcgccagaag tcatcatgac gttgtaggcg aataaattgc ccggcgcggt attccgacaa
    16681 ctgatacggt ccagtgccga ccggttgacg gtcgagttgc tcctggcgat cttctttctc
    16741 taacttccgg gcatattctg ccgacatgac cgaagcataa tgggttgcga ggtgccacaa
    16801 aaaagaagca tccggctgag ccagacggaa ctcaacggta tgattatcca gtttacggac
    16861 gcttttcacg ttatcggcaa attgcaggct gtcgaagtag gggaagttgc tgccgttgac
    16921 gttatgccac gggttgttgc ggtcaaaaat tcgctggaag gtaaacacca catcgtcggc
    16981 attcattttg cgagtgggag taaaccaggc ggttttttga aacgggacat cgcgacgcag
    17041 gtggaagcga taggtcgcgc cgttgtcgag tacttcccag ctttcggcaa gttctggcat
    17101 caggcgatag gtatagggat cgacatccag cagtcgatca taaaactggg cggcaagggt
    17161 atcgacaatt aacccactgc tcgctttgga tgggttaaag gtgttgactt gcccgctgac
    17221 gcaatagaca aaaccgctgt cgcggatatc agcatgcggg ggggattcag gcgcggcgat
    17281 tgcctgacca ctcacaagtc cagcaatcac caaaagagac gataatacct ggcgcataat
    17341 attaagggat tttatgtaaa gaggctatct tactaatatt taatgacatt ttccattacc
    17401 gtttgtgttc aggggtcgta atgagggccg tgtggttggt cgttgaacag atggcgtcgc
    17461 catctgttca acattcgtac ccgtgatttc tctataacta taactcacag aacaacttag
    17521 cgaggagcag agcggtacga tcgtgagaaa cccattaaag cacgctgcag tgcgtgtcat
    17581 tgttagccgg atgcggcgtg aacgctttat ccggacaacg atactgaccg atcgtctgca
    17641 attaaataat tactcattac cccattgatt caaaaactct gcgatctcat caatgcgtac
    17701 gggattaatc cccgcttcag cagccatttc atgctgggct tcttcgctga tctcttcatt
    17761 gttcatcaaa cgggtgagca gtaactggaa gtagtgggcc agcggtgtgc gttcagcgtc
    17821 tgcgacaggc tctgcgcatt cggtcgcgta ctcatcggca atatcaaaat attttagcgg
    17881 gatatcgtgg ttcattattt gcccctgggg ttaatgcgct aagcataagg gcggatgata
    17941 gcactcgtga tccgggtcat acatcttttt atagttaatg gcgcggcgca ttaccctcag
    18001 gcaggataat gcggcgcgca tccgactatt acgtttcgct cactggcgtt ctgtcacctt
    18061 cgtacagcgg cactttgcga tagcggcgga tcaaatacca caaatatgcg ccgcctaacg
    18121 aagcccatac cagaccgagt gtcagtgacg ttgactcgag gttaacccac agcacaccca
    18181 ccgtcagcgc gccaaccagc ggcatcagca aatagtggaa gtgatccttc cagcttttat
    18241 tcattccttt acgccgccag aaatgattaa acaccgacag attcacgaag gtaaacgcca
    18301 ccagagcacc gaagttaatc aatgctgtcg cggtgactaa atcaaagaac aatgccgaca
    18361 tcgcgacaat tccgaccata atgacgttca gtgccggagt ccgccatttt gggtgcacat
    18421 agccaaacac gcgctccgga aacacattgt cgcgccccat cacatacagc agacgtgaca
    18481 cgctggcatg tgaggccagg ccagacgcta acgtgttcac aaacgtggtg cagaggaaaa
    18541 tcgactggaa cagcttaccg ccgacgtaga gtgcaatttc aggcagtgcg gcgtccgggt
    18601 ctttaaagcg gctgatatcg gggaagaaca gctgcataaa gaacgacgcc gcgataaaga
    18661 taacgccacc atagaccgcc gtcaggaaga tggctttcgg gatcacgcgt gcggcgtctg
    18721 gtgtctcttc cgaaagtgtg gtcaccgcat cgaaaccgag gaacgagaaa cagacaatcg
    18781 tcgccccggt aataatcggg atcaggtgcg cgttctcgct gataaacggc tgaagtgacc
    18841 agacggtgcc aacgccttct cctttatgca gtccctgaac caccaggaag ataaacacca
    18901 ccatgatgga gatttgcacc agtacaaaca gggtattgaa gttagcgacc aggttgacgc
    18961 tcttcagatt cgcggcggtt aaaatggcga cgaaggttac cacccacacc cacggcggca
    19021 cttccgggaa gagggcggag agatagattt tcgccaacaa aacgttgatc atcggcaaaa
    19081 agagataatc cagcagtgat gaccagccga ccataaatcc gacgtgcggg ttaatcgact
    19141 tttgcgcgta ggtataggcc gaaccggcct ccggaaactg gcgaaccagt ttgccgtagc
    19201 tgatagcggt aaacagcacg cccgccagcg ccagcaaata ggacgcaggg acgtgaccgt
    19261 cgctaatgcc agatacaatg ccaaaggtat cgaacacagt catcggcgtg agataggcca
    19321 gacccatcat cacaacctgc cacaatttca gtgattttcg cagacgggtt ttgccgggtt
    19381 gcgcagcaat attcagtggt gaattaatag ccataattgc tgcctcccct gcgtaataag
    19441 gtttgcggtc gtgtcgacct gaaatcaaac ctgtttagca agggaagcaa agggggcgga
    19501 gtccagtcag tcgcaggagg ggcgcatacg cagcctcccg cgccatgcgg ggatttaatc
    19561 atcggcatca tctcatttcc tcgtcacagt cttaatcgtt aacggaagcc cgttgctgcc
    19621 tgcgctccat aacgcgacat ccgggaaatg tctggattac cagcaaatgt caggcctggc
    19681 tccgctcagg ccgatgaaac aaccccgcaa ggggtactat gcgtttttca acatccactc
    19741 aatttctgtt tctgtgatga ggcgctcaaa ctgcaacagc tcatcatttt tacaggcgtg
    19801 atacacatgg caaaaccgtt cgcctaaata gcggcgcagg tgatcattct cgataaactc
    19861 acccagggca tcgctctggc gaatcgggaa gggtaagcct tcctgttcca gcccgttacc
    19921 ttcgacttct tcctgcaacg gcagctcgtt atcaaggcca tgcaaaatac cggcaaaaat
    19981 cgctgccatc accagatatg ggttggcatc ggcacccgcc acgcgatatt ccacgcggtg
    20041 attatgacga tcgccgcatg gaatacgcag ggcgacggta cggttattat ggccccacga
    20101 cgcctgcgtc ggtacataca tccccggctg gaagcggcga tacgagttca cgtttggtgc
    20161 cagcaacgcc attgatgacg gcatcaggtc aatcatcccg gcgagcatct ttttcagcag
    20221 cggcgaatct tcgccttctg tgtcagaaag cacgttctcg ccacgattat tttgcatact
    20281 gatatggata tgcattccgc tgcccgcgtg ctcttcatac ggcttcgcca taaaagtggc
    20341 gtgcatctta tgcttttctg ccatcagacg caccaggcgt tttagtgcca gcgcatcatc
    20401 gcaggcttcc agcacgttat cagtatggta aaggttgatt tcaaactgac ccggcgaggc
    20461 ctcagcgacc gcgccatctg ccggaatcag ctgtaactgc gccagttcat caatatcatt
    20521 gagcacgtcg gcgaagtggt tgaggttatc aacggagtaa acctggcttt gtgtattgcg
    20581 gtcatcagtg ccgggcgcgc agggcggttg cagataccct tcagcgtcgc gctggcgatc
    20641 cagtaaatag aactccagct ctaccgctac gaccgggaac aatccgcgct ggcgcagctg
    20701 ctgccacagg cggttaagaa cgttccgcgg ctcaacgtca aagggagcgc catcttcatc
    20761 gaccatggtc aggagcatct gaccgataaa ctctggatcg gcggcagaag gggttaagga
    20821 accgagaaca ggaacacagg tacgatccgg ctcgcccatt tcctgaccca gacctgcttc
    20881 ttctactacg ttgcccagaa tatccattgc aaataccgag gccgggaaat aacacccttt
    20941 ctcgagcttc ttcaggcttg aaacaggaat acgttttcca cggaagcaac cattcaaatc
    21001 ggtaagtaaa acatcaacat attgcgtatt cgggtagcgc tccaggtagc gtttcacttc
    21061 ctgcgtaaag gcgctacccc gcctctcttc tgactgctga acaaagttct ctacttcaac
    21121 gatattggtt tccatgattc ttcgcctttg gtttgttttc cgctcgttat caaagcgtaa
    21181 aatataatga ccaccattcg aatctgtatg caaactaaat gtttgtcaaa tgttaaattg
    21241 agtttgcaaa aatgaaaacc cactgctaga ttgaaaaaat attgaacata aaggtcattt
    21301 aaaccgcagt aatggcgata atttagtcca ctttgtgaga ttgagaatgg aaaatataat
    21361 gaacaatccg gttatcggtg tcgtaatgtg caggaacagg cttaaggggc atgcgaccca
    21421 gactctgcaa gaaaagtacc tgaatgccat catccacgca ggcggcttgc caattgcgct
    21481 gccacatgcg ctggcggaac cgtcgttact tgaacaactt ttgccgaaac tcgatggcat
    21541 ttaccttcct ggtagtccca gcaatgtgca gccgcaccta tatggtgaaa acggcgatga
    21601 gcctgacgcc gatcccgagc gtgatcttct gagcatggcg ttaattaatg ccgcactcga
    21661 aaggcgcatc cccattttcg ccatctgccg gggtttacag gaactggtgg tggcaaccgg
    21721 tggttcgttg catcgcaagc tgtgcgaaca gcctgaattg ctggaacatc gggaagatcc
    21781 cgaactgccg gtggaacagc aatatgcacc gtcgcatgaa gttcaggttg aagaggggga
    21841 ttgctgtctg cattgttacc tgaatgtagc aacttttggg taaactctct gcatggacaa
    21901 ggggcgaagg tcgttagccc acggttgcgt gttgaagctc gctcgccgga tggcttagtt
    21961 gaggcggtta gtgtcatcaa tcatcctttt gcgctgggcg tacagtggca cccggaatgg
    22021 aacagtagcg agtacgcgct ttcgcgtata ttgttcgagg gctttatcac cgcttgtcag
    22081 caccatatcg ctgaaaaaca gcgactctga ccactacagt ttaaggaaat gcaaatatga
    22141 gtgatgaggg actggcgcca ggaaaacgct tgtcggaaat ccgccagcag caggggcttt
    22201 cacaacgtcg tgccgccgaa ctctccgggc tgactcacag tgctatcagt acgatagaac
    22261 aagataaagt cagccctgcc atcagtacgc tgcaaaagct gctgaaggtg tatggtctgt
    22321 cactctcgga attcttttcc gagccggaaa aacctgatga gccgcaggtc gtcattaatc
    22381 aggacgattt aattgagatg ggcagtcagg gtgtgtcaat gaagctggtt cataacggta
    22441 acccgaatcg cacgctggcg atgatctttg aaacgtacca gccgggcaca accactgggg
    22501 aaagaattaa gcatcagggt gaggaaatag gcactgtact ggaaggtgaa attattctga
    22561 cgattaatgg tcaggattac cacctcgtcg cggggcaaag ttatgccatt aataccggca
    22621 tcccgcacag tttcagtaat acgtcggcag gtatttgccg aattatcagc gcccatacgc
    22681 ccaccacgtt ttaatctttt tgttctgtaa gccgggtaag cgcaatgcgc ccggcaatcc
    22741 ataaagaatg tcgccagaaa taaacgggca tacggcccgg ggatctctgc gccctgacgt
    22801 tcacaaactg catatatctg atagacgtga aacaggagtc ataatgaatt ttcatcatct
    22861 ggcttactgg caggataaag cgttaagtct cgccattgaa aaccgcttat ttattaacgg
    22921 tgaatatact gctgcggcgg aaaatgaaac ctttgaaacc gttgatccgg ttacccaggc
    22981 accgctggcg aatattgcgc gcggcaagag cgtcgatatc gaccgtgcgg tgagcgcagc
    23041 acgcggcgta tttgaacgcg gcgactggtc actctcttct ccggcaaaac gtaaagcggt
    23101 gctgaataaa ctcgccgatt taatagaagc caacgccgaa gagctggcac tgctggaaac
    23161 tctcgacacc ggcaaaccga ttcgtcacag tctgcgtgat gatatacccg gcgcggcgcg
    23221 cgccattcgc tggtacgccg aagcgatcga caaagtgtat ggcgaagtgg cgaccaccag
    23281 tagccatgag ctggcgatga tcgtgcgtga accggtcggc gtgattgccg ccatcgtgcc
    23341 gtggaacttc ccgctgctgc tgacttgctg gaagcttggc ccggcgctgg cagcagggaa
    23401 cagcgttgtc ctgaaaccgt ctgaaaaatc accgctcagt gcgattcgtc tcgcggggct
    23461 ggcgaaagaa gcaggcttgc cggatggtgt gttgaacgtg gtgacgggtt ttggtcatga
    23521 agcggggcag gcgctgtcgc gtcataacga tatcgacgcc attgccttta ccggttcgac
    23581 ccgtaccggg aaacagctgc tgaaagatgc aggcgacagc aacatgaaac gcgtctggct
    23641 ggaagcgggc ggcaaaagcg ccaacatcgt tttcgctgac tgcccggatt tgcaacaggc
    23701 ggcaagcgcc accgcagcag gcatcttcta caaccagggg caggtgtgca tcgccggaac
    23761 gcgcctgttg ctggaagaga gcatagccga tgaattctta gccctgttaa aacagcaggc
    23821 gcaaaactgg cagccgggcc atccacttga tcccgcaacc accatgggca ccttaatcga
    23881 ctgcgcccac gccgactcgg tccatagctt tattcaggaa ggcgaaagca aagggcaact
    23941 gttgttggat ggccgtaacg ccgggctggc tgtcgccatc ggcccgacca tcattgtgga
    24001 tgtagacccg aatgcgtcct taagccgcga agagattttc ggtccggtgc tggtggtcac
    24061 gcgtttcaca tcagaagaac aggcgctaca gcttgccaac gacagccagt acggccttgg
    24121 cgcggcggta tggacgcgcg acctctcccg cgcgcaccgc atgagccgac gcctgaaagc
    24181 cggttccgtc ttcgtcaata actacaacga cggcgatatg accgtgccgt ttggcggcta
    24241 taagcagagc ggcaacggtc gcgacaaatc cctgcatgcc cttgaaaaat tcaccgaact
    24301 gaaaaccatc tggataagcc tggaggcctg aaatgacaga acataccagc agttactacg
    24361 ccgccagtgc gaataaatac gcgccattcg acacgctgaa tgagtcaatt tcctgcgacg
    24421 tttgcgtggt tggcggcggc tataccgggc tgtcttccgc actgcatctg gcggaagcgg
    24481 gctttgacgt ggtggttctg gaagcctcac gcatcggctt tggcgccagc gggcgcaacg
    24541 gcgggcagct tgtgaactcc tacagccgcg acatcgacgt tatcgaaaaa agctacggca
    24601 tggacaccgc ccgtatgctc ggcagcatga tgttcgaagg tggtgagatc atccgcgaac
    24661 gtatcaaacg ttatcagatt gactgcgact accgccccgg cggtctgttt gtggcgatga
    24721 atgataaaca gctcgccaca cttgaagagc agaaagagaa ctgggaacgc tacggcaata
    24781 aacagctgga gatgctggac gccaacgcca ttcgccgcga ggtcgccagc gatcgctaca
    24841 ctggtgcgct gctggatcac agcggcgggc atattcaccc gctgaacctc gccattggcg
    24901 aagcggacgc tatccgcctg aacggcgggc gcgtgtatga gctttccgca gtgacgcaga
    24961 tccagcacac cacgcccgcc gtagtgcgta cagccacagg tcaggtgacg gcgaagtatg
    25021 tgattgtcgc cgggaatgcg tacctgggcg ataaagtgga gccggaactg gcgaaacgca
    25081 gcatgccgtg cggtacgcag gtgatcacca ccgagcggct ttccgatgat ttagcccgtt
    25141 cgctgatccc gaaaaactac ggcgtggaag actgtaatta tctgcttgat tactaccgtc
    25201 ttaccgccga caaccgtttg ctgtacggcg gcggcgtggt gtacggcgcg cgcgacccgg
    25261 atgacgttga gcgtcttgtg gtgccgaaac tgctgaaaac cttcccgcag ttgaagggcg
    25321 tgaaaatcga ctatcgctgg acgggcaact tcctgctgac cctgtcgcgt atgccgcagt
    25381 tcggtcgcct cgataccaac atctattaca tgcagggtta cagcggccac ggcgtcacct
    25441 gtactcatct cgccggacgt ttgattgccg aactgctgcg cggcgacgcc gaacgtttcg
    25501 atgccttcgc caatctgccg cattacccgt tccccggcgg gcgcacgctg cgcgtaccgt
    25561 ttaccgcgat gggcgcggcg tattacagcc tgcgcgatcg tctgggcgtt taatttccga
    25621 ttaacagtga agagtcaaaa ggtgtgaaac atgagcaaca atgaattcca tcagcgtcgt
    25681 ctttctgcca ccccgcgcgg ggttggcgtg atgtgtaact tcttcgccca gtcggctgaa
    25741 aacgccacgc tgaaggatgt tgagggcaac gagtacatcg atttcgccgc aggcattgcg
    25801 gtgctgaata ccggacatcg ccaccctgat ctggtcgcgg cggtggagca gcagctgcaa
    25861 cagtttaccc acaccgcgta tcagattgtg ccatatgaaa gctacgtcac cctggcggag
    25921 aaaatcaacg cccttgcccc ggtgagcggg caggccaaaa ccgcgttctt caccaccggt
    25981 gcggaagcgg tggaaaacgc ggtgaaaatc gcccgcgccc ataccggacg ccctggcgtg
    26041 attgcgttta gcggcggctt ccacggtcgt acgtatatga ctatggcgtt aactggaaag
    26101 gtcgcgccgt acaaaatcgg cttcggcccg ttccctggtt cggtgtatca cgtaccttat
    26161 ccgtcagatt tacacggcat ttcaacacag gactccctcg acgccatcga acgcttgttt
    26221 aaatcagaca tcgaagcgaa gcaggtcgcg gccattattt tcgaaccggt gcagggcgag
    26281 ggcggtttca acgttgcgcc aaaagagctg gttgccgcca ttcgccgcct gtgcgacgag
    26341 cacggtattg tgatgattgc cgatgaagtg caaagcggct ttgcgcgtac cggcaaactg
    26401 tttgcgatgg atcattacgc cgataagccg gacttaatga cgatggcgaa aagcctcgcg
    26461 ggcgggatgc cgctttcggg tgtggtcggt aacgcgaata ttatggacgc gcccgcgccg
    26521 ggcgggcttg gcggcaccta cgccgggaat ccgctggcgg tggctgccgc gcacgcggtg
    26581 ctcaacatta tcgacaaaga gtcactctgt gagcgcgcca tgcagttggg gcagcgtctg
    26641 acgaaaaccc tgattgatgc caaagaaaac gttccggcca tcgcggcggt acgtggtctg
    26701 ggctcgatga ttgcggcaga atttaacgac ccgcaaacgg gcgagccgtc agccgccatt
    26761 gcgcaaaaaa ttcagcaacg cgcgctggcg cagggactgc ttctactgac ctgtggcgca
    26821 tacggcaacg tgattcgttt cctgtatccg ctgaccatcc cggatgcgca attcgatgcg
    26881 gcaatgaaaa ttttgcagga tgcgctgaga gattaatacg cagaagcagg ggcaacgcaa
    26941 taatgcctga tatgctgcgc ttatcagacc tgcaaacgac gtattgatta tgtatgccgg
    27001 ataaggcgtt cacgccgcat ccggcaagtt gtattgccca acttcgctaa atctggtgct
    27061 ttttcaacaa cgcgcggaac tgatgatagg ttaagcccag caattcagct gcgcgcttct
    27121 ggttatattt cccttgttgc aaactgagtt gcagcaactc tttttcctgc tgcatctgaa
    27181 actcacgtaa atccagcggc agtgttggaa gcgaggtggt ttctgaaacg gcgatagcgt
    27241 cttcaggcgg acgccgttta aagggatcaa taatgatgtc atcaagcgga taatcgctgg
    27301 tgccgtggcg atacactgaa cgttccacca cgtttttcaa ttcacgaata tttcctggcc
    27361 aacgataatt cagcaatgtt tctctggcgc gctccgtaaa ccccgggaac agaggcagct
    27421 tgatttcccg acacatctgg atggcaaagt gttccgccat taacataatg tcgctttcac
    27481 gttcgcgtag tggcggcagt tgcactacat cgaaagccag ccggtcgagc aggtcagcgc
    27541 gaaaagtacc ttcattgacc atcgccggga gatcggcatt cgtcgcgcat accaaccgca
    27601 cattcacctg caacggctgg ctgccgccaa cgcgctccag ttcaccgtac tcaatcacgc
    27661 gcaataattt ctcctgcacc atcatcggtg ccgtagcgag ttcatcaaga aatagcgtac
    27721 cgccatcggc acgttcaaat ctccctggat gacgtttttg cgcaccggta aacgcccccg
    27781 cttcgtgacc aaacagttcg gaatccagca gattttcatt taacgccgcg cagttaaggg
    27841 aaataaacgg cccttgccaa cgggaggaga gataatgcag gcggctggcg atcagctctt
    27901 taccggtgcc gcgttcgccg atgatgagca ccggtttgtc cagcggtgcg agatgcgaaa
    27961 cctgttccag cacttcgaga aagctgttcg cctcaccgag taaattatct ttgtattctg
    28021 ccatgatgaa attcgccact tgttagtgta attagctaat tcatcctggc atgttgctgt
    28081 tgattcttca atcagatctt tataaatcaa aaagataaaa aattggcacg caaattgtat
    28141 taacagttca gcaggacaat cctgaatgca gaaatcaaga ggacaacatt atgggtattt
    28201 tttctcgctt tgccgacatc gtgaatgcca acatcaacgc tctgttagag aaagcggaag
    28261 atccacagaa actggtgcgt ctgatgatcc aggagatgga agatacattg gttgaagtac
    28321 gttctacttc ggcgcgtgcg ctggcagaaa agaaacagct gactcgccgt attgaacaag
    28381 cgtcggcgcg tgaagttgaa tggcaggaaa aagccgaact ggcgctgctg aaagagagag
    28441 aggatttggc gcgtgcggcg ttaattgaaa aacagaaact gaccgatctg attaagtccc
    28501 tggaacatga agtgacgctg gtggacgata cgctggcacg catgaagaaa gagattggcg
    28561 agctggaaaa caaattgagc gaaacacgcg ctcgccagca ggcattgatg ttacgccatc
    28621 aggcggcaaa ctcgtcgcgc gatgtgcgtc gtcagctgga cagtggcaaa ctggatgaag
    28681 caatggctcg tttcgaatct ttcgaacgtc gtattgacca gatggaagcg gaagcagaaa
    28741 gccacagctt cggtaaacaa aaatcgctgg acgatcagtt tgccgaactg aaagccgatg
    28801 atgcaatcag cgagcaactg gcacaattaa aagccaaaat gaagcaagac aatcaataat
    28861 aacatccagg cggcgtccga acgcgccgcc gctcatcgtc taaggagtac ttatgagcgc
    28921 gctatttctg gctattccat taaccatttt tgtgctgttt gttttaccga tctggttatg
    28981 gctgcattac agcaatcgtt ctggtcgcag tgaattgtcg caaagtgagc agcagcgatt
    29041 agcgcaactg gctgatgaag caaaacggat gcgcgaacgt attcaggcgc tggaatctat
    29101 tcttgatgca gaacatccga actggaggga tcgctaatgg cgggcattaa tctcaataaa
    29161 aaattatggc gtattccaca gcagggcatg gtccgtggcg tctgcgccgg gattgccaac
    29221 tattttgatg taccggtaaa actggtgcgt atcctggtgg tgttgtcgat tttcttcggt
    29281 ctggcgctgt ttaccctggt tgcttacatc attttgtcat ttgcgcttga tccaatgccg
    29341 gacaacatgg cctttggtga gcagctacct tccagcagtg aattgctgga tgaagtcgac
    29401 cgtgaactgg cggcaagtga aacgcgttta cgcgagatgg aacgttatgt cacttccgat
    29461 acattcacgt tacgtagccg tttccgtcaa ctgtgaggaa agttatgaat actcgctggc
    29521 aacaggccgg gcaaaaggta aagcctggtt tcaaattagc aggcaagctg gtacttctta
    29581 ccgcactgcg ctatggccca gcgggtgtgg cgggctgggc gataaaatca gttgctcgcc
    29641 gaccgctgaa aatgttgctg gctgtggcgc tggaaccgct gttaagtcgg gctgccaata
    29701 aactggcgca gcgttataaa aggtgagagg agtttcgcaa aaattgttaa atctcaggcg
    29761 tataatggat agcaatattc atccatagaa ggacgcttac atgtttaaaa aaggcttact
    29821 tgctctggca ctggtgtttt cactgcccgt tttcgccgct gaacactgga tcgatgttcg
    29881 tgttccagag cagtatcagc aagagcacgt tcagggggcc atcaatattc ccctgaagga
    29941 agtgaaagag cgcattgcca ccgtcgttcc ggataaaaac gacaccgtga aagtgtattg
    30001 caatgccgga cgccaatcag ggcaggcaaa agagatcctt agcgagatgg gatataccca
    30061 cgttgagaac gccggtggcc tgaaagacat cgcaatgccg aaggtcaaag gttaaaagaa
    30121 ttcaccatga gcggcgctta tgcgccgttt ttttctgtca caccttattt acatccctat
    30181 agattatttg cgtcagctca caaatacgct ttttccctgg taaaaaatga tttcctgcgt
    30241 gactaaaacc cttgtgctca attgacagtt tattttctgc ggagtagtct ctcatttcat
    30301 gggaccgcta ccaggaaagg caacatgaaa cagaaaatta cggattacct ggacgaaatc
    30361 tacggtggaa catttaccgc aactcattta cagaaacttg taacgcgtct tgagagtgcg
    30421 aaacgattaa ttacacagcg acgtaaaaaa cactgggatg aaagtgatgt cgtgttaatt
    30481 acctatgccg atcaatttca cagtaatgat ttaaaaccac tacccacatt taatcagttt
    30541 taccatcaat ggctgcaaag cattttttca catgttcatt tattaccttt ttatccatgg
    30601 tcatctgatg atggattttc ggtaattgat tatcatcagg tcgccagtga agcgggtagt
    30661 ggcaggatat tcagcagctc ggtgaatgca gtcatttaat gtttgatttt gtctgcaacc
    30721 atatgtcggc aaaaagtgaa tggtttaaaa actatttaca acagcagcca ggttttgaag
    30781 atttttttat tgcggttgat ccgcaaaccg atctcagcgc tgtcacccgt ccgcgtgcat
    30841 taccgctatt aacgccgttt caaatggatg ataattccac gcgccattta tggaccacct
    30901 ttagcgacga tcaaattgac ctgaattacc gtagccctga cgtgttgctg gcgatggtgg
    30961 atgttttgct ttgttacctg gaaaagggcg cagaatatgt ccgtctggat gccgttggct
    31021 ttatgtggaa agagccggga acaagctgca tccatctgga aaaaacacat ctgattatca
    31081 aactgttacg gtcgattatt gatgacatcg cgccaggtac ggtgattatt actgagacga
    31141 atgttccgca taaagataac attgcttact ttggcgaagg cgatgacgaa gcgcatatgg
    31201 tgtaccagtt ctcgctgccg ccgctggtgt tacatgcggt gcaaaaacag aacgtcgagg
    31261 cgctgtgcca gtgggcgcaa agtctgagct tgccctccgg taaaacgacc tggtttaact
    31321 tcctcgcctc tcacgatggc atcgggctga acccactgcg gggcttgtta ccagaaagcg
    31381 aaatattagc gctggtcgag gcattacagc aggaaggtgc attagtaaac tggaaaaata
    31441 atcccgacgg tacgcgcagt ccctatgaaa taaatgtcac ctatatggat gcgttaagcc
    31501 gccgcgagag tagcgatgaa gaacgttgcg ccaggtttat ccttgcccat gcgattttgt
    31561 taagtttccc cggtgtgcca gcgatatata ttcaaagtat tctgggctcg cgtaatgatt
    31621 acgcaggtgt cgaaaaactc ggatataacc gtgcgattaa ccgtaaaaaa tatcacagtg
    31681 aagagataac ccgagaactg aacgatgaag ctacattaag gcatgcggta tatcatgagt
    31741 tgtcgcgttt aattacactt cgtcgcagcc ataacgagtt tcatccggat aataatttta
    31801 ccatcgatac ggttaattca tccgtaatgc gtattcaaag aagtaacgca gagggtaatt
    31861 gtctgaccgg attgtttaat gtcagtaaaa atattcagca tgtaaatatt actggcctgc
    31921 acggtcggga tctgattagt gaagttgata tattgggtaa taaaataacg ctgcgcccct
    31981 ggcaggttat gtggattaaa taaaaaggaa catctcatga ttaaatcaaa aatcgtgctg
    32041 ttatcagcac tggtttcctg cgccctgatt tcaggctgta aagaagaaaa taaaccccat
    32101 gtatccatcg aatttatgca ttcttcggtg gagcaggagc gtcaggcagt tatcagcaaa
    32161 ttgattgccc gttttgaaaa agaaaaccca ggtatcaccg ttaaacaagt gcccgtcgaa
    32221 gaggacgctt ataacaccaa agtcatcact ctttcacgta gcggctcgct gccggaagtg
    32281 attgaaacca gtcatgacta cgccaaagtt atggacaaag agcagcttat cgatcgcaag
    32341 gcggttgcca cggtcatcag caacgttggt gaaggcgcgt tttacgatgg cgtactgcgt
    32401 attgtgcgta ccgaagatgg tagcgcatgg accggtgttc ctgtcagcgc ctggattggc
    32461 ggcatctggt atcgcaaaga tgtgctggca aaagcggggc tggaggagcc gaaaaactgg
    32521 caacagctgc tggacgttgc acagaaactg aatgacccgg cgaataaaaa atacggtatt
    32581 gcgctgccta cggcagaaag cgtgctgacg gaacaatcct tctcccaatt tgcgttatcc
    32641 aactaggcca acgtctttaa cgccgaaggc aaaatcaccc ttgattcacc agagatgatg
    32701 caggcgctga cctattaccg cgaccttgct gccaacacca tgccgggttc taacgacatc
    32761 atggaagtga aagacgcctt tatgaacggc accgcgccga tggcgattta ctccacctat
    32821 atccttccgg ctgtgattaa agaaggcgat ccgaaaaacg tcggtttcgt ggtgccaacc
    32881 gagaaaaact ctgccgtcta cggcatgttg acctcgctga ccattaccgc cggacaaaag
    32941 actgaagaaa cggaagcggc tgaaaaattt gtcaccttta tggagcaggc agacaacatt
    33001 gccgactggg tgatgatgtc gccaggcgcg gcgctgccgg tgaacaaagc ggttgtcact
    33061 acttccacct ggaaagacaa cgacgttatt aaggcgctgg gtgaactgcc aaatcagtta
    33121 atcggtgaac tgccgaatat tcaggtcttt ggcgcagtag gggataaaaa atttacccgc
    33181 atgggcgatg tgacggggtc tggggtggtg agctccatgg tgcataacgt caccgtaggt
    33241 aaagccgatc tccctggcac gctgcaaacg agccagaaaa agctggatga actgatcgaa
    33301 cagcactaaa cccaggacag gaatccgcaa tgaacaggct tttttcaggt cgttccgata
    33361 tgccctttgc gctgttgctt ctcgcaccca gcttattact gctgggcggt ctggttgcgt
    33421 ggccaatggt gtcgaatatc gaaattagtt ttttacgtct gccgctcaat cccaacatcg
    33481 agtcaacgtt tgttggggta agcaactatg tgcgtattct ctccgatccc ggcttctggc
    33541 actcgctgtg gatgacggtc tggtataccg cgttggtggt ggcgggcagc accgtgctcg
    33601 ggctggcagt ggcgatgttt ttcaaccgcg agttccgcct gcgcaaaacc gcgcgttcgc
    33661 tggtgatcct ctcctacgta acgccgtcta tttcgctggt gttcgcctgg aaatacatgt
    33721 tcaacaacgg ctacggcatt gtgaactacc tcggcgtcga tcttctgcat ctttatgagc
    33781 aggcaccgct gtggttcgat aatccgggca gtagttttgt gctggtggtg atcttcgcca
    33841 tctggcgcta cttcccgtat gcctttatct cgtttctggc gattttgcag accatcgaca
    33901 aatcgctgta cgaagcggca gagatggatg gcgctaatgc ctggcaacgg tttcgtatcg
    33961 tcacgctgcc cgcgattatg ccggtcctgg cgacggtggt gacccttcgc accatctgga
    34021 tgttctacat gttcgcggat gtttatctgc tgacaaccaa ggtcgatatt cttggtgtct
    34081 atctctacaa aaccgccttt gcctttaatg acttagggaa agcggcggcg atctcggtgg
    34141 tgctcttcat cattattttc gctgtcattc tgctgaccag gaaacgggtg aacctcaatg
    34201 gcaacaaata aacgcacact cagtcgtatc ggtttttacc gcgggctggc gctgtttctc
    34261 atcatcacgc tgtttccgtt ttttgtgatg ctgatgacct cgttcaagag cgcaaaagag
    34321 gcgatctcgc tgcatcctac gctgctgccg caacagtgga cgctggagca ttatgtcgac
    34381 atttttaacc cggtgatttt cccgtttgtc gactacttcc gtaacagcat ggtggtgtcg
    34441 gtggtttcat ccgtggtggc ggtatttctc ggcattctcg gagcttacgc gctttctcgc
    34501 ctgcgcttta aaggccggat gaccatcaac gccagctttt acacggtgta tatgttctcc
    34561 ggcattttgc tggtggtgcc gctgttcaaa attatcaccg cacttggcat ttacgacacc
    34621 gagatggcgc tgatcattac catggtgacg caaactctgc ccaccgccgt gtttatgctg
    34681 aaaagttact tcgacaccat ccctgatgag atcgaagaag cggcgatgat ggatggcctc
    34741 aaccgtctgc aaattatctt ccgtatcacc gtgcccctgg cgatgtccgg gctgatatcc
    34801 gtattcgtct actgctttat ggtggcatgg aacgactatc tgtttgcgtc gattttcctc
    34861 tccagcgcca gcaatttcac cttaccagtc ggcctgaacg cgctgttcag tacgccagat
    34921 tacatctggg gacggatgat ggcggcctcg ctggtgaccg cattgccggt ggtgattatg
    34981 tatgcgcttt cggaacgttt tattaagagt ggtttgaccg ccggtggcgt gaagggctaa
    35041 agcggccagt ttctttacaa ggagttttac atgaaaaagt tagtagccac agcaccgcgc
    35101 gttgctgcgc tggttgagta tgaagatcgg gcgattttag ccaatgaagt gaagatccgc
    35161 gtgcgtttcg gtgcaccgaa acacggaacg gaagtggtcg acttccgcgc cgccagcccg
    35221 tttatcgatg aagactttaa cggcgaatgg cagatgttca ccccgcgtcc ggcagatgcg
    35281 ccgcgcggca ttgagtttgg caaattccag cttggcaaca tggtggttgg cgacattatc
    35341 gagtgcggca gcgacgttac cgactacgcg gtgggcgaca gcgtatgcgg ctacggcccg
    35401 ctctccgaga cgatcatcat taacgcagtg aataactaca agctgcgcaa aatgccggaa
    35461 ggcagctcct ggaaaaacgc tgtctgctac gacccggcgc agtttgccat gagtggcgtt
    35521 cgcgatgcca acgtacgcgt aggggatttt gtagtggtgg tagggcttgg cgcgatcggt
    35581 caaattgcca tccaactggc taaacgcgct ggcgcgtcgg tggtaattgg cgtcgatcct
    35641 attgcccatc gttgtgatat tgctcgtcgt cacggtgcgg atttctgcct taatcccatt
    35701 ggcactgacg taggcaaaga gatcaaaacg ctgaccggca agcagggtgc cgatgtgatt
    35761 atcgaaacca gcggttacgc cgacgcgctg cagtcggcgc tgcgcggcct ggcttacggt
    35821 ggcaccatct cctatgtcgc gtttgctaaa ccgtttgctg aaggttttaa cctcggacgc
    35881 gaagcgcatt tcaataacgc caaaattgtc ttctctcgcg cgtgcagtga accgaacccg
    35941 gattatccgc gctggagccg taagcgtatt gaagaaacct gctggaaact gctgatgaac
    36001 ggttatctca attgcgaaga tttaatcgac ccggtagtga cctttgccaa cagcccggaa
    36061 agctacatgc agtatgtcga tcagcatccg gaacagagca tcaaaatggg cgtcacgttt
    36121 taattcaaag gaatgaaaaa atgaaaatag gcacacagaa tcaggcgttc ttcccggaaa
    36181 atattctgga gaaatttcgt tatatcaaag agatgggctt cgatggtttt gagattgacg
    36241 gcaaactgct ggtcaacaac atcgaagaag tcaaatcggc gatcaaagaa accggtttac
    36301 cggtgaccac cgcctgcggc ggctatgacg gctggattgg tgactttatc gaagagcgtc
    36361 gtcttaatgg cttaaagcag atcgaacgca ttctcgaagc gctggcagaa gtggggggta
    36421 aaggtatcgt cgttccggca gcgtggggca tgtttacctt ccgcttaccg ccgatgacct
    36481 cgccgcgtag cctggacggc gaccgcaaaa tggtgagtga ttccctgcgc gtactggaac
    36541 aggtcgccgc gcgtaccgga accgtggtgt atctcgaacc gttaaaccgc tatcaggatc
    36601 atatgatcaa caccctcgcc gatgctcgtc gttacagcgt cgaaaacaat cttaaacatg
    36661 tgcagattat cggcgatttc tatcacatga acatcgaaga agataacctg gcgcaggcgc
    36721 tgcatgacaa ccgcgacttg ctcggtcatg tgcatattgc cgataaccat cgttaccagc
    36781 cgggcagcgg caccctcgat ttccacgcgc tgtttgaaca gctgcgcgcc gataactatc
    36841 agggttatgt ggtgtatgaa gggcgtatcc gggcagaaga tcctgcccag gcgtaccgtg
    36901 attcgttgac ctggttgcgt acctgctaag aggtctttgt gaaaagtgca atgacaagct
    36961 ctccactgcg ggtcgcgata ataggcgcag gccaggtggc ggataaggtt catgcttcgt
    37021 actactgcac ccgcaacgat ctggaactgg tggctgtctg tgacagccgc ctttcccagg
    37081 cgcaggcgct ggcagaaaaa tacgggaatg catccgtgtg ggacgatccg caggccatgc
    37141 tgctggcggt gaaacctgat gtggttagcg tctgctcacc taaccgtttt cattacgaac
    37201 ataccctgat ggcgctggaa gcgggctgcc atgtgatgtg cgaaaaaccg cccgccatga
    37261 cgccagaaca ggcgcgggaa atgtgcgata ccgcgcgcaa acagggcaag gtgctggcct
    37321 acgactttca ccatcgtttt gcgctcgata cgcaacagct gcgtgaacag gtgaccaacg
    37381 gcgttttggg agagatttac gttaccaccg cccgcgccct gcgtcgctgc ggcgttcccg
    37441 gctggggtgt ctttaccaat aaagaactgc agggtggtgg cccgctgatc gacatcggca
    37501 ttcatatgct ggatgctgcg atgtatgtgc tgggctatcc tgcggtgaaa agcgtgaatg
    37561 cgcatagctt tcaaaagatc ggcacgcaaa agagctgtgg tcaatttggc gagtgggatc
    37621 cggcaactta cagcgtcgaa gattcgctgt ttggcaccat tgaatttcat aacggcggca
    37681 ttctgtggct ggaaacgtca tttgcgctca acatccgcga acagtcgatt atgaacgtca
    37741 gcttttgtgg tgataaagcc ggtgcgacgc tgtttccagc acatatctac accgataaca
    37801 acggtgaatt aatgacgctg atgcaacggg aaatggcaga cgacaaccgc catttgggca
    37861 gcatggaagc ctttatcaat cacgtacagg gcaagcccgt gatgatagcc gacgccgagc
    37921 aggggtacat catccagcaa ctggtggcgg cgttatatca atccgcagaa acagggacgc
    37981 gtgtggaatt atgaccaggc cagtaacgtt atcagaaccc catttcagcc agcataccct
    38041 gaacaagtat gcatcgctga tggcgcaggg gaacggctat cttgggcttc gcgccagcca
    38101 tgaagaagat tacacgcgcc agacgcgagg gatgtatctg gcggggctgt atcatcgggc
    38161 gggaaaaggt gaaatcaacg aactggtgaa cctgcctgat atcttgggga tggagattgc
    38221 cataaatggt gaggttttct cgttatccca cgaagcctgg cagcgtgagc ttgactttgc
    38281 cagtggcgaa ttacgccgca acgttgtctg gcgtaccagc aacggcgcag gttacaccat
    38341 cgccagccgt cgctttgttt cggcagacca actgccgctc attgcgctgg aaatcactat
    38401 tacgccactg gacgccgacg cgtcagtgct gatttcaaca ggtatcgacg ccacgcaaac
    38461 caaccacggt cgccaacatc tcgacgaaac ccaggtgcgg gtgtttggtc agcatctgat
    38521 gcaggggatc tacaccaccc aggatggacg cagtgatgtg gccatcagct gttgctgtaa
    38581 ggtgagcggt gatgtgcagc aatgctatac cgccaaagag cgccgtttgc tgcaacatac
    38641 cagtgcgcag cttcatgcag gcgagacagt gacgttgcaa aaactggtgt ggatcgactg
    38701 gcgggatgac aggcaagccg ttttagacga gtggggcagc gcgtcgcttc gccagcttga
    38761 aatatgcgcg cagcagagtt acgaccaact tcttgcagca tcaacagaaa actggcgtca
    38821 atggtggcag aaacgtcgta tcacggtaaa tggcggcgat gcgcacgatc agcaagcgtt
    38881 agattatgcg ctttatcatc tgcgcatcat gacgccggct cacgacgagc gcagcagtat
    38941 tgcggcaaaa ggcttaaccg gcgaaggcta caaaggccac gttttctggg atacagaagt
    39001 atttttgctg ccgttccatc tgtttagcga tccgacggtt gcccgaagtt tactgcgtta
    39061 tcgctggcac aacttgccag gcgcgcagga gaaagcacgg cgcagcggct ggcagggcgc
    39121 gctatttccg tgggaaagcg cgcgcagcgg cgaagaagag acgccagaat ttgccgccat
    39181 taacattcgt accgggctgc ggcaaaaagt ggcctcggcg caggcggaac atcatctggt
    39241 ggccgatatc gcctgggcgg ttattcaata ctggcagacc acgggggatg aaagtttcat
    39301 tgctcatgaa ggcatggcgc tacttctgga aactgcaaag ttctggatta gccgcgcggt
    39361 gagggttaac gaccgtctgg aaattcatga tgttattggg ccagacgaat ataccgaaca
    39421 tgtcaataat aacgccttca ccagctatat ggcccgctac aacgttcaac aggcgctgaa
    39481 tattgcccgc cagttcggct gtagcgacga tgcgtttatc catcgcgccg aaatgttcct
    39541 caaagagcta tggatgccag aaacgcagcc cgatggcgtt ttgccgcagg atgattcgtt
    39601 tatggctaag ccggcgatta atctggctaa atacaaagcg gcggcgggga agcaaaccat
    39661 tctgctggat tattcacgcg cagaagtgaa cgagatgcag atcctcaaac aagctgatgt
    39721 ggtgatgctc aattacatgc tgccggagca gttctcagcg gtatcgtgtc ttgccaatct
    39781 gcaattttat gaaccgcgca ctattcacga ctcgtcatta agtaaagcaa tccacggcat
    39841 tgttgccgca cgctgtggcc tgctgaccca aagttatcag ttctggcgcg aggggactga
    39901 aatcgatctt ggtgctgatc cgcatagttg tgatgatggt atccacgctg ccgcaactgg
    39961 cgctatctgg ctgggggcga ttcagggttt tgccggggtg agcgtgcgtg acggtgaatt
    40021 acatctcaat ccggcgttac cggagcagtg gcaacagttg tctttccctc tgttctggca
    40081 gggctgcgaa ttacaggtca cgctcgacgc gcagcgtatt gcgattcgaa cttctgcgcc
    40141 cgtttcactg cgtttgaacg ggcagcttat atccgtggct gaagaatctg ttttctgttt
    40201 gggtgatttt attttgccct tcaatgggac cgctaccacg catcaggagg atgaatgaaa
    40261 ctgcaagggg taattttcga tctggatggt gtaatcaccg ataccgcgca tctgcatttc
    40321 caggcgtggc agcagattgc cgctgaaatt ggcatcagca ttgatgcgca gtttaacgaa
    40381 tccctaaaag ggatcagccg cgatgagtct ctgcggcgca ttctgcaaca cgggggcaaa
    40441 gagggcgact ttaacccgca ggagcggacg caactggcgt atcgcaaaaa tctgctttac
    40501 gtccactcac tgcgcgagct gacggttaat gctgtgctgc ccggtattcg caatttgctg
    40561 gcagagctcc gcgcacagca gatcccggta gggctggctt ctgtctccct gaatgcgccg
    40621 acgattttag cggcgctgga gctgcgcgag tttttcacct tctgcgcgga tgcttcccaa
    40681 cttaaaaact cgaaaccgga tccggaaatc tttctcgctg cctgtgccgg gctgggcgtg
    40741 ccgccgcagg catgtatcgg cattgaagat gcgcaggcgg gcattgacgc cattaacgcc
    40801 agcggtatgc gctcggtggg gatcggcgca ggcttaaccg gggcgcaatt attgttgccc
    40861 tcaacggact ccctgacctg gccgcggtta tcggccttct ggcaaaacgt atagcaaagg
    40921 aatcaacatg gctcagcttt cgttacaaca tattcaaaaa atctacgata accaggtgca
    40981 tgtggtgaag gacttcaacc tggaaattgc cgataaagag ttcatcgtat ttgtcggccc
    41041 gtcgggctgc ggtaaatcga ccaccctgcg catgattgcc gggcttgagg agatcagcgg
    41101 cggcgatctg ttgatcgacg gcaaacgaat gaatgacgtt ccagccaaag cacgcaatat
    41161 cgcaatggtg ttccagaact acgcgttgta tccgcatatg acggtttacg acaacatggc
    41221 gtttggcctg aagatgcaaa aaatcgccaa agaggtgatt gatgagcggg tgaactgggc
    41281 ggcgcaaatt ctcggcctgc gtgagtacct gaagcgcaag ccgggcgcgc tttccggcgg
    41341 gcaacgccag cgtgtggcgc tcgggcgggc gattgtgcgc gaagcgggcg tgtttttaat
    41401 ggatgaaccg ctctctaacc ttgatgccaa gctgcgcgta caaatgcgcg cagagatcag
    41461 caagctgcat cagaaactga acaccaccat gatctacgtg acccacgatc agaccgaagc
    41521 gatgaccatg gcgacgcgga ttgtgattat gaaagacggg attgttcagc aagtgggtgc
    41581 gccgaaaacc gtttataacc aacccgcgaa tatgtttgtt gccggattta ttggatcacc
    41641 agcgatgaat tttattcgcg gcacgatcga tggcgataaa ttcgttacgg aaacgcttaa
    41701 attaaccatt cccgaagaga aattagcggt tctgaaaaca caggaaagtt tgcataagcc
    41761 catcgtgatg ggaatgcgcc cggaagatat tcatccggac gcgcaagagg aaaataatat
    41821 ttccgccaaa attagcgtgg tagaattaac cggtgcggaa tttatgctct acaccacggt
    41881 tggggggcac gagttagtgg tccgtgctgg tgcgttaaat gattatcatg caggagaaaa
    41941 tatcactatt cattttgata tgactaaatg tcatttcttt gatgcagaaa cggaaatagc
    42001 aattcgctaa atacaggggg aaggcattcc cccaggataa tacaaggaac aataatgaaa
    42061 aagttattac cctgtaccgc actggtgatg tgtgcgggaa tggcctgcgc acaggccgag
    42121 gaaaagaacg actggcactt taatatcggc gcgatgtacg aaatagaaaa cgtcgagggt
    42181 tatggcgaag atatggatgg gctggcggag ccttctgtct attttaatgc cgccaacggg
    42241 ccgtggagaa tttctctggc ctattatcag gaagggccgg tagattatag cgcgggtaaa
    42301 cgtggaacgt ggtttgatcg cccggagctg gaggtacatt atcagttcct cgaaagcgat
    42361 gatttcagtt tcggcctgac cggcggtttc cgtaattatg gttatcacta cgttgatgaa
    42421 ccgggtaaag acacggcgaa tatgcagcgc tggaaaatcg cgccagactg ggatgtgaaa
    42481 ctgactgacg atttacgttt caacggttgg ttgtcgatgt ataaatttgc caacgatttg
    42541 aacacgaccg gttacgctga tacccgtgtc gaaacggaaa caggtctgca atataccttc
    42601 aacgaaacgg ttgccttgcg agtgaactat tatctcgagc gcggcttcaa tatggacgac
    42661 agccgtaata acggtgagtt ttccacgcaa gaaattcgcg cctatttgcc gctgacgctc
    42721 ggcaaccact cggtgacgcc gtatacgcgc attgggctgg atcgctggag taactgggac
    42781 tggcaggatg atattgaacg tgaaggccat gattttaacc gtgttggttt attttacggt
    42841 tatgatttcc agaatggtct ttccatttcg ctggaatacg cgtttgagtg gcaggatcac
    42901 gacgaaggcg acagcgataa attccattat gcaggtgtcg gcgtaaatta ctcgttctga
    42961 taatgggcta aattgccgga tgcggcgcga gtactttatc cgatctataa atgtaggccg
    43021 gataagatgc gccagcatcg catccggcat tcaggcaagg tcgctggtat ttatttcagc
    43081 gtcatatgcg tggcaacggt aatattttgt ggtgacggtt ttccggcaat taagcgaaat
    43141 aataactcac agctttgtcg acctaactcc tgcgtcggaa catcgatgcc gcccggtgca
    43201 ggcgttaaaa taaatgacag cgtttcattg ctataaccca ccaccgctaa ctgctgcgga
    43261 atagcaatgt ttttctctgc cgccgcacga taaatgctca ttaatttcag gctgtcagtg
    43321 gcaaacactg cctcaggcaa cggtgactga cttaataatt gccgtgctgc ttgcagtgct
    43381 gtttcatggg tataaccgcc atcaacaatc cattcatcac gcactgcaat attatgatct
    43441 tccagactct gcttataacc atttacccga tccactgaaa catggacatc aagcggtgca
    43501 tgcagacagg caatattttg atgtccactt tcaattaacg catcggtcaa cgcaatgctg
    43561 tcaccaaaat tatcggtatc gacagaataa acatgggcat attgaccttc aactttgcca
    43621 atcaccacca caggaatatc gtatttatca agttgggcaa aaaatgactc atccgctggc
    43681 gaacttagca taataatgcc tttaatcatt ttctgcttaa ttttgctttc acatttttgt
    43741 aagtcttctg ccggggtgtg cgacgtctgc aatatcacgt cgaaaccttc ttcttcagct
    43801 ttggcggtga tggcatgcaa aacttcagag aaaaacggat tacccgccgt agttttggtc
    43861 gaacgggtag aaatcaccat aatggcatca aaacccgaag aggtcagcgc gcgggccagc
    43921 ttgtttggtt gatactgtaa ttcttcaatg gcccgtaaca ctttttcgcg cgcttccggg
    43981 gagatattgg tttgcttatt cagcacgcgt gatacggtgg attttgatac gcctgcaacc
    44041 ctggcaatat cataaatagt aggggacata ggtcaggaac tccgtccgaa cgtgaatgct
    44101 gcacatctta tggagttcgc ccgtcgatga caacacgggt agttgataat caatggcctg
    44161 gcccccacat tcatatcctt atgaatgatt tttttctggt cttcagagca gcacaggaca
    44221 gcaatgaagc gacttaaaaa tgaacttaat gcgctggtga atcggggtgt cgacagacat
    44281 ctgcgcctcg ccgtaaccgg acttagccgc agcggcaaaa cggcgtttat cactgcgatg
    44341 gtcaatcagt tgctcaatat tcatgccgga gcacgtttgc cgctattaag tgcggtgcgt
    44401 gaagagcgtc tgctgggcgt aaaacgcatt cctcagcgtg actttggcat tccgcgtttt
    44461 acctacgacg aagggttggc gcagctgtat ggcgatcctc ccgcctggcc gacgccaacg
    44521 cgcggcgtca gcgaaattcg cctggcacta cgctataaat cgaacgattc gctgctgcgc
    44581 cactttaagg atacctccac gctgtatctg gagattgtgg attaccctgg cgaatggttg
    44641 ctcgacctgc cgatgctggc gcaggactat ttaagctggt cgcgccagat gacgggctta
    44701 ctcaatggtc agcgcggcga atggtcggcg aaatggcgaa tgatgtgcga agggctggac
    44761 ccgctagcgc ctgccgacga aaaccggctg gcagacattg ccgccgcgtg gaccgattat
    44821 ctccaccact gtaaacagca ggggctgcac tttattcagc ctgggcgctt tgtcttgccg
    44881 ggggatatgg caggtgcgcc cgcgctgcaa ttcttcccgt ggccggatgt cgatgcctgg
    44941 ggcgagtcca aactggcgca ggccgataag cacaccaatg ccggaatgct gcgcgagcgg
    45001 tttaattatt actgcgagaa ggtggtgaag gggttctata agaatcattt tctgcgcttt
    45061 gaccgccaga ttgtgctggt ggattgcctg caacctctca acagtgggcc acaggcattt
    45121 aatgatatgc gtctggcgct gacgcagctg atgcaaagtt tccactatgg gcagcgtacc
    45181 ctgttcaggc gtttgttttc gccggttatc gataagctat tgtttgctgc cactaaagcg
    45241 gaccatgtga ccatcgatca gcacgccaat atggtttcat tgctgcaaca actaattcag
    45301 gatgcctggc aaaatgcggc gttcgaaggg atcagcatgg attgcctggg gctggcgtca
    45361 gttcaggcga ccaccagtgg cattattgat gttaacggtg agaaaatccc ggcgctgcgc
    45421 ggtaatcgac ttagcgatgg cgcaccgctc actgtttatc ctggcgaagt tcccgcacgt
    45481 ttgcctggtc aggcgttctg ggataagcaa gggttccagt ttgaagcgtt tcgcccgcag
    45541 gtgatggatg tcgacaaacc gctgccgcat attcgtcttg atgccgcgct ggaattttta
    45601 ataggagata aattgcgatg accgaaccgt taaaaccacg tattgatttc gacggtccgc
    45661 tggaggtcga acagaatcca aaattcaggg cgcagcagac ctttgacgaa aatcaggcgc
    45721 aaaattttgc cccggccacg ctcgacgaag cgcaggaaga agaggggcaa gtcgaagcgg
    45781 taatggacgc agcgttacgt ccgaaacgca gcctgtggcg caaaatggtg atgggcgggc
    45841 tggctctgtt tggcgcaagc gttgtcgggc agggtgtaca gtggacaatg aatgcctggc
    45901 aaactcagga ctgggtggcg ctgggtggat gtgctgctgg ggcattgatt atcggcgctg
    45961 gcgtaggttc tgtggtaaca gagtggcggc gcttatggcg cttgcgacag cgcgcccatg
    46021 aacgcgacga agcgcgcgat ttgttgcaca gccacggcac gggcaaaggc cgcgcatttt
    46081 gcgaaaaact ggcgcagcag gcgggtattg atcagtctca tccagcgctg caacgctggt
    46141 atgcctcaat ccatgaaacg cagaacgatc gtgaagtggt cagcctgtat gctcatctgg
    46201 tccagccggt tttagatgcc caggcgcggc gcgaaatcag ccgctcagca gctgaatcaa
    46261 cgttgatgat tgcggtcagc ccgctggcgc tggtggatat ggcatttatc gcctggcgca
    46321 atctgcgttt gattaatcgc atcgccacgc tgtatggcat tgaactggga tattacagcc
    46381 gtttgcgcct gttcaagctg gtattgctga atatcgcttt cgccggagcc agcgaattgg
    46441 tgcgcgaagt gggaatggac tggatgtcgc aagatctcgc tgctcgtttg tctacccgcg
    46501 cagctcaggg gattggtgca ggacttctga cggcacgact ggggattaaa gctatggagc
    46561 tttgccgccc gctgccgtgg attgacgatg acaaacctcg cctcggggat ttccgtcgtc
    46621 agcttatcgg tcaggtgaaa gaaacgctgc aaaaaggcaa aacgcccagc gaaaaataat
    46681 gcaatatcgg gtgctgaccg gatatcttta cgccgaagtg cccgtttttc cgtctttgtg
    46741 tcaatgattg ttgacagaaa ccttcctgct attcaaatag tgtcatatca tcatattaat
    46801 tgttcttttt tcaggtgaag gttcccatgc gtctggaagt cttttgtgaa gaccgactcg
    46861 gtctgacccg cgaattactc gatctactcg tgctaagagg cattgattta cgcggtattg
    46921 agattgatcc cattgggcga atctacctca attttgctga actggagttt gagagtttca
    46981 gcagtctgat ggccgaaata cgccgtattg caggtgttac cgatgtgcgt actgtcccgt
    47041 ggatgccttc cgaacgtgag catctggcgt tgagcgcgtt actggaggcg ttgcctgaac
    47101 ctgtgctctc tgtcgatatg aaaagcaaag tggatatggc gaacccggcg agctgtcagc
    47161 tttttgggca aaaattggat cgactgcgca accataccgc cgcacaattg attaacggct
    47221 ttaatttttt acgttggctg gaaagcgaac cgcaagattc ccataacgag catgttgtta
    47281 ttaatggaca gaacttcctg atggagatta cgcctgttta tcttcaggat gaaaatgatc
    47341 aacacgtcct gacaggtgcg gtggtgatgt tgcgatcaac gattcgtatg ggccgtcagt
    47401 tgcaaaatgt cgccgcccag gacgtcagcg ccttcagtca aattgtcgct gtcagcccga
    47461 aaatgaagca tgttgtcgaa caggcgcaga aactggcgat gctaagcgcg ccgctgctga
    47521 ttacgggtga cacaggtaca ggtaaagatc tttttgccta cgcctgccat caggcaagcc
    47581 caagagcgag caaaccttac ctggcgctga actgtgcgtc tataccggaa gatgcggtcg
    47641 agagtgagct gtttggtcat gctccggaag ggaagaaagg attctttgag caggcgaacg
    47701 gtggttcggt gctgttggat gaaataggtg agatgtcacc acggatgcag gcgaaattac
    47761 tgcgtttcct taatgatggc actttccgtc gggttggcga agaccatgag gtgcatgtcg
    47821 atgtgcgggt gatttgcgct acgcagaaga atctggtcga actggtgcaa aaaggcatgt
    47881 tccgtgaaga tctctattat cgtctgaacg tgttgacgct caacctgccg ccgctacgtg
    47941 actgtccgca ggacatcatg ccgttaactg agctgttcgt cgcccgcttt gccgacgagc
    48001 agggcgtgcc gcgtccgaaa ctggccgctg acctgaatac tgtacttacg cgttatgcgt
    48061 ggccgggaaa tgtgcggcag ttaaagaacg ctatctatcg cgcactgaca caactggacg
    48121 gttatgagct gcgtccacag gatattttgt tgccggatta tgacgccgca acggtagccg
    48181 tgggcgaaga tgcgatggaa ggttcgctgg acgaaatcac cagccgtttt gaacgctcgg
    48241 tattaaccca gctttatcgc aattatccca gcacgcgcaa actggcaaaa cgtctcggcg
    48301 tttcacatac cgcgattgcc aataagttgc gggaatatgg cttaagccag aagaagaacg
    48361 aagagtaagc gcgaatatgc ctgatggtgc aacaccatca ggcatattaa cttatgcttt
    48421 cagtacagcc agagctgctt cgtaatccgg ctcggtggtg atttcatcca ccagctggct
    48481 gaaaatcaca ttgtcatttt catcaataac cacaacggca cgcgctgcca gacctttcag
    48541 tgggccatca gcaattgcca caccgtaggc ttgcagaaat tcagcgttac ggaaagtgga
    48601 gagggtgata acgttgttca gaccttctgc gccgcagaaa cgagactggg cgaacggcag
    48661 atcggcagag atacacagca caacggtgtt gtcgatctcg gttgccagtt ggttaaactt
    48721 acgtactgat gcggcgcaaa caccggtatc aatactcggg aaaatgttca gcactttgcg
    48781 tttacccgca aactgaccga gggtgacgtc agacagatct tttgccacga gagtaaaagt
    48841 ctgcgctttg ctacccgcct gcgggatgga attggcgact gtaaccgggt tgccctggaa
    48901 atgaacggtt tgtgacatga ttatctttcc tgtttacata tagttaacgt cacacctagt
    48961 ttatgctaac tgtcaataac acagcaaacg ctatttgcgc ttaatccgca gaccaccgcg
    49021 acaccaagga gtaaagatga gaaccgttaa ggtatttgag gaagcctggc ccttacatac
    49081 cccgtttgtg attgcccggg gaagtcgcag tgaagcgcgc gtggtggtgg ttgaactgga
    49141 agaagagggt attaaaggca ccggcgaatg cacgccgtat ccgcgttatg gagaaagtga
    49201 tgcctcggta atggcgcaaa ttatgagcgt cgtgccgcaa ttagagaaag ggctgacacg
    49261 ggaggagttg caaaaaattc tccctgccgg cgcagcacgt aatgcgctgg attgtgcatt
    49321 gtgggatctg gccgcgcgaa aacagcagca atcgctggct gatttgatcg gcataacgct
    49381 tcccgagaca gttatcactg cacagacggt agtcatcggt acgcctgatc agatggccaa
    49441 tagtgcatca acactctggc aggccggcgc gaaattactg aaagtgaagc tggataacca
    49501 tcttatcagt gagcggatgg tggcaattcg cacagctgtg cccgatacca cgctgatcgt
    49561 tgatgcaaat gaatcctggc atgcagaagg gttggcggca cgttgccagc tattggcgga
    49621 tttaggcgtt gcgatgcttg aacaaccgct tcctgcgcag gacgatgcgg cgctggagaa
    49681 ttttattcat ccgttgccga tttgtgctga tgaaagttgt catactcgta gcaatttgaa
    49741 ggcgctgaaa gggcgctatg agatggttaa tattaagctc gataaaaccg ggggtctgac
    49801 ggaagcgctg gcgctggcga ctgaagcgcg tgcacaaggt ttcagtctga tgctgggctg
    49861 catgttgtgt acctctcggg ccattagcgc cgctttaccg ctggtgccgc aggtcagttt
    49921 cgccgatctt gacggaccga cctggctggc ggtagatgtg gaaccggcgc ttcagttcac
    49981 gacgggcgaa ttgcatcttt aggatgccag cgcagcaagt ttgccatcgc aaagagatat
    50041 ttttcgctgg cttcgtcgga ggagatgggc ggaaactctg cggtgatgca atgcaagttc
    50101 agatctgcac accaactgcc gaaagagcca ggcgtttcat aaccgacgct ggttaccaga
    50161 ggcaattcaa acgcctgagc cagccattcg cctaattcgc tgtgtctggg atcttcaata
    50221 caggccagtg gatcgtggaa tgagaccacc caggcaggtt gaatgcggtg tataagctgg
    50281 cacagcgcct gggtttcagg ttcggagcca ggtttgtcac ctgtcagtaa tacgacatca
    50341 cgttcttcag cggcgctgtt ccagcgataa accgtttcac cttccttcca gttcgccgcc
    50401 ggaaagtttc tgtttaaatc cacaccatta gcattggccc gtaaccccag ctggcagccg
    50461 tcaggattca cacacagcac cacatgatgg cggcgcaaag aaggtgtcaa agtccgcaga
    50521 gcacaggaga gcgtgacgac cgaagagttt tcatcaccgt gagtgccagc cagaatcaaa
    50581 ccactttcgc gactggctgc aggggccgga aaccagatta acggcgcgcc caataatgaa
    50641 cgtccgtaat gttctgttcc gggcggaaat gcgccgcgtt cggcgcgtgg gcgggttacg
    50701 gtcatgatcg tctctggttc aggaagtatt acaggcagtg ttgtgcaaat tttgcggatt
    50761 atcaaatcct tttcttcaac gctatgtcat cgatcttgta tcactttttt gccggaagtt
    50821 gtttgtattt tcattgatgt gcagatatta gaattgtcgt aatgttatat acatggcgta
    50881 ttttattctt actggttcta cacatgttaa tcgcatctga ttgatcagtt caccttaata
    50941 agtttaataa atcgtactgc acatagtaca gtacgatggt ggttatataa ttgatttaca
    51001 tgaagaatgg cttgtcgcca tcagtcccgg gttgattgat gcctatattc tcaccaatgt
    51061 gtttctcagg tttatcgatg atatctgtaa ttaatgctgc gacactctta ggtgatacga
    51121 tcgtaccttt gaatggttca ttgcgtgagg taagctcata atcaataata tcttcatccg
    51181 tcagccaggc agggcgaaga attgtatatt ccagtccaga agcctcaatt gcatccgcag
    51241 ccctgcggaa tggttttagg ggctcaccaa taactgcatt attccattcg ccaaattttc
    51301 ctggaacttc atcataaatg ccgagggaaa gaacaaagat cagacgttta acatcacagg
    51361 ctttcatcgc agcgatcact gaatttgcct gaatatctaa atcttccccc gtaaggttgg
    51421 catacacaat atcctgtccc tgcatggcct gcttcagtgc tgcatgattc agtacatccc
    51481 ccataataat ctggctatta gttggatagg gtttgtgtat ttttgctggc tgacgagcaa
    51541 ataaagtttg tttaatggtt tgtttatctg ccagttgatt aatcacatgg cgggctatct
    51601 gaccgccagc accaagaatg agtacatttt tcataatatt tctcatgaaa cttatcacag
    51661 atgggtgttg aaaaaaggaa taactttttc cagcgcctcc tgaactgctt ccggtttgtc
    51721 atacagttca taatgtgaga aatcgggtaa tgaaactaac tgacgatcct gcgaaaccat
    51781 cgcccggcca tagacttcca gtccatcacg ataagcaccg aaggcaccga ctttttcacc
    51841 aactactacc atcaccggct gagtcagtaa cacttcagta aatgcgaagg catcccaggc
    51901 cagcgttttc tgtgcatgtg agaacaacat ttttgtcgca cctcccggtt gttgcccgcg
    51961 tggtgtttta tagtagtcag ttgcttcata aacatcgcgt tcggtcagtc catgggcttt
    52021 tgctgcatcc aggctggcgg gaagtaattc gttgatctgt aattctccac cacgagcctc
    52081 tttggttcgt tgcgacgcca ttgcattcaa tgcgccaatc ggatcgtaat tgctgaaacc
    52141 ttcccgaaaa agacgcccga tattaacacc cgtaatactc actacagctt taattcgttt
    52201 ttctgtcagc gcggcattaa tggcatagcc accccccccc acacacaccc agcacgccaa
    52261 tgcgttcagc atcaacatat ggcagagtta ctgcataatc gataacacgg ctaatatcct
    52321 ctacgcgctg tgtcggatct tctatccaac gaggctcccc accagattct ccctggaaac
    52381 tggcgtcata agcgagaacg acatagcctt tttcagccaa tgccttgcca tagatattgc
    52441 cagacgtctg ctccttacaa ctgccaaaag gatggacgct gataatcgcc ggatactgtt
    52501 ttgactcatc aaaatttggt ggaaactgaa tattggcagc aatatgccag gccatatcac
    52561 tattgcggat ttgaatatgt ttcattacag agtttcctca aagaacggag ctaatactga
    52621 aattgcttca gccacatagg ctttgccgtc ataaagatcc atatggtttg cgccttcaac
    52681 aatgtgatag cgtttatcct ggcttgatgc ccgatcgtac aggtcgtcac tcatccattt
    52741 gctccctgcc tggctgcccg ccacaatctg cgtcggctga gtcaggtaca cttccgccat
    52801 atggtaagca tcataggtaa taatctggtt aaggctgcgc aaagtagcgt aacccggtgc
    52861 tgttggatac tgcgcgcgag gggtgtggta atactcccag gcctgacgca gttcttcgtt
    52921 cggcgcatcg gactccttca ttggtgccag tggcataatg gcgtattctc cgctgctaat
    52981 atcgctggtt ctggcgtttg aacccgcttc aacgtatggc agggcatcaa tagatttcac
    53041 attgttttcc caaccattac ggaacatcga accaatattg accgcactaa cggtaccgat
    53101 ggccttgatg cggcgatcct gaattgcagc attggctgta tatcctgcac cggcacaaat
    53161 tcccatcgca ccaattcggg tattgtcgac ataagaaagc gttgtcaggt aatcaatcac
    53221 ggcactgacg tcttcagtac gaatgtatgg gttttctaac tgacgcggct cgccgccact
    53281 ttcaccctga taagatgcgt cataagcaat agtgacaaat cccttttccg ccagtttttc
    53341 ggcataggtc ccggccgttt gttctttaac gcccccacct gggtgagata ccacaattgc
    53401 cggatattga cgggtttcat caaattttgg aggaaaatag atcactgcag acaaagagat
    53461 ggttggatta ttgctgttag tgaagctgac tttattattc atcattccgt tcctcatgga
    53521 gttgttggtt cgttttaacg gttggtgata tcactataga tattgatcat taagttgatt
    53581 agaccctaaa tcatgattag acctatcatt taaatgatta atagattgta tggagatact
    53641 gaatgaagcg tgaagagatc gctgatctga tggcgtttgt cgtcgttgca gaggagcgta
    53701 gcttcactcg cgcagcagcc cgcctgagca tggcgcagtc agctttaagc cagatagtgc
    53761 gccgtataga agaacgattg ggattgcggc ttctgacgcg aaccacgcgc agtgttgtac
    53821 ccactgaagc gggcgagcat cttttgtctg ttcttggccc tatgctgcat gacatagatt
    53881 ctgccctggc atccctgagc gatctgcaga accgcccatc cgggacaata cgtattacta
    53941 ctgtagaaca tgcagcaaaa acgatattgt taccagccat gcgcacattc ctgaaatcac
    54001 accctgaaat tgatattcag cttatcattg attatggttt gactgatgtc gtttctgaac
    54061 gttttgatgc aggcgtccgt ctgggtgggg agatggataa agatatgatc gccattcgaa
    54121 tcgggccaga tataccaatg gctattgttg gctcaccgga ttatttttct cgccgaagtg
    54181 ttccaacgtc agtgtcacaa ttaatagatc atcaggcaat taatttgtat cttcccacat
    54241 cgggtacagc aaatcgctgg agattaatac gcggtggacg tgaagttcgt gttcgcatgg
    54301 aaggtcagct tttactgaat acgatagacc tgatcattga tgctgcaatt gatgggcatg
    54361 gattggcgta tctaccttat gatcaggttg agcgggcgat taaagaaaaa aaactgatac
    54421 gtgttttgga taaattcaca ccagatttac ccggttatca cctgtactat ccacaccgtc
    54481 gacatgctgg ctcggcattc tcattattta tagataggct gaagtataaa ggtgctgttt
    54541 agcactactt gctgatacat taatttaatt cttctcttaa cgtattctca gttcctttca
    54601 acgttttggt tattttttat tcttcgtaca atggcgacag atgctgatta tgataccgaa
    54661 aactggtttg aacgtgtgaa gcccgaacgt agtgttcggt gttctatgtg ctttgccgca
    54721 ttttggcgtc tattatttac actaaatctg atttaatata ttgatacata aaacatttta
    54781 tgcttcctgt gtcactttct gatggaagtt gtttgcattt ctttaaggcg aaacaaataa
    54841 ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg tttcagtcac
    54901 gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag ttccgagcgg
    54961 cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc ctgcgtcgct
    55021 ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc tgtttgaagg
    55081 actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc agtggaaaag
    55141 taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg cggatggcac
    55201 accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc cgaaaacgct
    55261 atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg cgattattga
    55321 tggtaaagct acacctgacc agcttggcgt caccgcagtt gatgtccata ctttgaaaat
    55381 tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg ccttcttccc
    55441 ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa atctgatcgg
    55501 caatggcgct tatgttctta aagagcgcgt agtcaatgaa aaactggtcg tggtaccgaa
    55561 tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc tgccaattaa
    55621 tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca ccgaatcctt
    55681 cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt atacgccgcc
    55741 gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg cagatcagcg
    55801 cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa aagtattagg
    55861 gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat ttacgccgga
    55921 accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa aaactttgtt
    55981 gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt ataacacttc
    56041 agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga accttggcgt
    56101 agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta acaccggcaa
    56161 ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca ctttcctgac
    56221 attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg catacgacaa
    56281 agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg attacaacgc
    56341 ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat ataccaatgg
    56401 acgattaatc aaaccgtggc tgaaaggtta tcccattaat aaccctgagg atgtggcgta
    56461 cagtcggact atgtatattg tgaagcattg atgtgatggg aactggcgtt acccttgtgg
    56521 ataacgccag tgatgtctga tttagcgacc ctgttccggc ggtgtaatgt tatccatata
    56581 cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgtacaatat cgataatctt
    56641 caaataaacg tcttgctgtg cagcaagcca ctcagcccat accgtggttt tggtaaagca
    56701 ataaaccata atattcaatg acgagtcagc aaactggttg aaataaacca gtaaggtttg
    56761 tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga cgataacgcc
    56821 cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc ggttggtcat
    56881 tcgtcctggg ttttctacgc tgatcgatga aaacagcgag ttcggtacgt acaatggacg
    56941 attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg taccttcgat
    57001 atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga aatagagcat
    57061 gatcccggaa aagaaattac tcagaatatc tttgccagcc ataccgacag ccagaccacc
    57121 aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc cataaagcag
    57181 aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg cactggtgat
    57241 atcgcgaccc tttttaatct gctgtttttc aaactgatta atcagcaaaa atagcttaat
    57301 cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgcctg gagtgataaa
    57361 tttgagttta tagttttcta taacataatt aataatgcta ccaacagcac tgataattat
    57421 ggtgtagatt aaaaattgca ccgcatggaa taaaaagcct tttcttttac gatttccgcg
    57481 gcgaaaccaa aagctcatca gaatcaatgc tgcgcagcta ccgaaaataa tgaccagatt
    57541 aagcgcatta tttgtaaaca gttcagcgat cattgtttta tcaggctcct ccagataatt
    57601 gtcgtcatgc cggaaacccc tggcggggct attttaccgc gacaattcat tcagatcatc
    57661 aatagtcagg gaaggaagta gcaacattag ctaactaatc aatgcgttga ttgtaaatcc
    57721 agctaagagg tgaggttttc agagcagaca atggtgaaat gtcatggtat tgttacgttt
    57781 aggtaacaag aaatttgtct gcacaaggat tacatcatga ttatggcgaa actgaagtca
    57841 gcgaaaggga agaaatttct ctttggtttg ttggcggttt tcattattgc ggcgtcggtt
    57901 gtgactcgcg cgactatcgg cggcgttata gaacagtaca atattccgct gtcggagtgg
    57961 acgacatcaa tgtatgtgat tcagtcatcg atgatttttg tttatagcct ggtctttact
    58021 gtgttgctgg caatcccgtt gggaatttat ttccttggcg gcgaagagca gtaagtaaaa
    58081 aataggcccg ataactcggg ccttgtcagt tattgaggag tcgttaatcg tcttcttcgt
    58141 catccagttc aacgggtgtc tgatactggt caggtttaat gaccaacaga tcgcagcgaa
    58201 gatgatcaat cacctgttcc gccgtgttgc cgaggaatgc tgctgaaata ccggtgcgtc
    58261 ctaccgtgcc cagaaccaca atccccgcct gtaagtgctc cgccaaatca ggaatcacct
    58321 cttctggcag acctttttct acgtgcgtca tgttttcatt aatgccgaat ttctgccgca
    58381 gggctttcat tgccagcaaa tgttgcccac gaatggcatc gttataaacg ctcggatcaa
    58441 attccggcag ttcaatcgcg atattaattg gcgttaccgg ataagcgcca accagatgaa
    58501 cttcggtatg gttgacttgt tctgccagtt cgatcgtctc tttgaccagt ttttcattga
    58561 gcgcattatg atacggctct tcactggcga gattcaccgc caccagcgcc ttacctcctt
    58621 ccggccacgg ctggtctttc accatccaca ccgggcttgg gcatttgcgt aacaggtgcc
    58681 agtccgttgg cgtaaaaatc accgcttcca gacggtcatg ttggtgcgcc atttttagca
    58741 ccaaatcgtg cccgccgctg atcacttcct gaatgatggc ttcgaaagga cggttatgcc
    58801 agaccacttt aatttcaatg ggaacgccag cattgagata atattttgcc tgctcgtgga
    58861 tccaggctgt acgctggctg atgacgccct gacgcatagc ggtacgttcg tccggggaga
    58921 gcagggtggt catttcgtat gagaagtcat agatcggcaa aaaggcttta attttgccac
    58981 caatccgttg atgtaaataa acagctcgcc gcaatgctgg ttggtcgtcc tggttaggat
    59041 cgataacaac gagcatgttc tgatacatag ccatacaggg tctccttaca acaactgtca
    59101 acgcagtttg taattaaaag attaacccat atctggtgaa tgaaacagtg atgaaccttc
    59161 tgccagatca ataaatcaga aaaatttaat gatatgacag aaggatagtg agttatgcgg
    59221 aaaaatcagg caacgttacg cgtatgacca gcaagctggg ccagcgcatc gttattttcg
    59281 atggtgatgt atttaccttt gactgccagc ataccgcttt tctggaagcg acccagcaga
    59341 cggctgatgg tttctaccgt caggcccaga tagttaccga tatcgccacg agtcatcgtc
    59401 aggcggaatt cacgagggga gaagccgcgt tgggcaaaac gacgggacag gttgtagatg
    59461 aatgcagcca gacgttcctc ggcatttttc tttgacaaca gcaggatcat gtcctgatcg
    59521 cctttgattt caccgctcat cagacgcatc atctgctgac gcagattcgg cattttaccg
    59581 gacaaatcgt ccagcgtttc gaacgggatt tcacatacca tcgaggtttc cagcgcctgc
    59641 gcgaagctcg ggtgatggcc gctgccgatg gcgtcaaatc ccaccaggtc gcctgctaaa
    59701 tggaaaccag tgatttgctc gtcgccttgc tcagtgatgg tataactttt aatcgtaccg
    59761 gagcggatgg cataaagcga tttaagttca tcaccagcct taaacagcgt ctggcctttc
    59821 tgaataggct tcttccgctc aatgatatta tcaagctgat caagctcatg ttcgttgagt
    59881 gtgaacggga tgcaaagctg gctgatgctg caatcctggc aatggatagc acaaccgcca
    59941 gactgaatgc gccgtataat tcgcttttcc gggatcatag gtctgctcaa gccgtaattg
    60001 atatttgtca attttaacat ctttttaggg agcaagtaag tctaagcaaa ccttaacagc
    60061 agagaattcc gatattagat gtaaatatat gtctatctat ttgaaaaccc ttaagttgtt
    60121 aagggtaact ttacataaaa gtgtgaacaa gctggcacaa attgtttaat gtttacagca
    60181 aaagataacc ttcgtggcgc aataaccact cttttcgctg aactccgcct gcatatccgg
    60241 tcatggtgcc gtttcggcca ataacccgat ggcaaggtac gacgatgctg atgggattcg
    60301 atccgtttgc cgcaccaacg gcacgcgccg cgccaggacg gcccaattgc tcagccagtt
    60361 cgccgtaatg cattacctgc ccgcagggga tagtgcgtag tgttttccag acttcgcgct
    60421 gaaatggcgt cccccccgta gcagtgggaa gcgtatcaat aatgctaaga ttaccggcaa
    60481 aatattcacg aagcttgtcg cttaaaccgc ctggattggt ggcagaaatg cgctcatagc
    60541 cttctttgcg ataatggatg tccagcagct gcaccatgcg ttcgctgtac tcttcccatt
    60601 caaccgcccg caggcgaaat tgctcatcgc aaatcaccca cagtggaccc agtggtgtgg
    60661 caattttttc ttcaagtaat ctcagcatcc gttctctctt aagacaaacg tgggtaaata
    60721 cccggaccta tcggcaggcc cacaagatac cacgccagca acatcagcag ccataccacc
    60781 aaaaagataa gcggataggg caagactaac gaatagtaag tacccagttt cgcgtctggt
    60841 ttgtagcgtt gcaggaatcc aagaaacagt ggaacaaaag gagatactgg cgctaaaggc
    60901 aatacggatg agtcggcaat acgaaagagg atttgcgcaa atgccgggtg aaagccaagt
    60961 agcataaaca ttggtacgaa aatgggggcc agaatcgacc agattgcgga accgctggcg
    61021 ataaacatgc ataagaaaga ggaaagcaac gccagaccga caaacgccgg gatgccgcta
    61081 agccctgaac tttccaggat atcggtcagc cccacggcga tgaatttccc catgttgctc
    61141 cagttaaaca tggcgacaaa ctgggcgagg ggaaaaacca tcacgataaa tcccgccatc
    61201 tctttcatcg gttcaatcat taaatgcggt aaatccgcct gacgtcgaat tgtgcgggta
    61261 gcgatgccat aagccagcga gacaacaaag aaaaaaagaa tgatcagtgg cacgatacct
    61321 ttaataaagg gtgatggcat cacggtgtga ttaatcggat cgcgcaatat cccgttttcc
    61381 gggatcacca tcagcgcaat cgcagcaata aaaagtagcg atacacacct gctatgcgta
    61441 aaccaaaacg ctgacttcgg tcaatgtctg cagtttctca tcgctgtttc cctgccattg
    61501 acctaaccgt ggctcgatga ttttgtcggt tatcaggccg ccaacaatcg tcagtacgac
    61561 tacggagctg gccataaaat accagttatc aattacactg acgtgcattt gcggattgaa
    61621 cgcagctgcc gcttccgtgc tgatccccga cagcaacacg tcggttgtga caatcagtaa
    61681 attagccgta aagccgcaac ctacgcctgc aatggcagcc agtaaacctg caactggatg
    61741 cctgcccacc gccagaaaaa tcagcgcacc catcggtggc atgatcacta acgccgcatc
    61801 ggaagaaatg tggctgaaaa aagcaataaa cagcaccata taactggcgt agcgggcatt
    61861 aacatgcgat gccattttaa ccattagtgc tggcagtaag ccgacgcgct ccgccagacc
    61921 ggcacctaaa accagcgcca ggatcgcacc aagtggagca aaaccgctaa agtttttaat
    61981 aacattgggt aaaaaccagt gtaatccttc cacactgagc aggtttttca ccaccaccgg
    62041 cgtaccatcg gtcgggtttt tcgcactgac gccaaaggcc gacaaaattg ccgtcgtcac
    62101 catgagtacg ataatcaaat agataaagag caaaaaagga tggggaacct tgttaccaat
    62161 tctttcgacc cagccataga gcttcccgga ttgggaggac gacggtatgg atgacatact
    62221 catgggcatt cctcggttgt tgtgtttgcg ttgttgttgt tattttaaag gtgacggtgt
    62281 cacgtttttc gggatagggc agtgatacgg ttgcgtgtcc gtaacttgct gatgctcttg
    62341 ttggcattct tgcaatagcc ctgaatcaat gaagagattg agtgtggttg ctgccatagt
    62401 tttcgccgcc agcagcattc ctttatgagc aatagatgtt cgcccctggc taaccagttg
    62461 ccacgtatgt aggggtgtac cgacggcaaa acaggggctg aaacactggg caacaggcag
    62521 tttccaactg acgtcgccga catcagtcga tgccgcaagc acgttatcgg tggcggcata
    62581 tggagcgact tcattcgcca gtaccgtttc acgatgacgt agtgcaaaaa ccttgccgtt
    62641 ttcgccaccg gttgcggcga tattattcag actgttttgc cgatcgttgg aggtaagcgt
    62701 agcctgaatt tgtttcgcaa aagccagttc ttcggagttc cattccgggg taccaaaatg
    62761 ggatagggcc tggtacatgg cattttctaa ggtgcgattc gggagataac tggaacaggc
    62821 tttgtcgaag cggcattcaa ccgtggtttc ggtcatcaat gccgcacctt cggcgatttt
    62881 ggcgacccga tcataaatat gctgcacgtc ggtcatttcg ggggcgcgga taagataaag
    62941 cacttctgcc tgcgcctgga ccacgttggg cgagatcccg ccactatttg tgatggcata
    63001 gtgtacgcgc gctttttcaa taatatgttc gttgaggaag ttggtgccag tggtcatcaa
    63061 cgttacggca tcaagggcgc tgcgtcccaa atgaggggaa ttcgcggcat gtgctgcgat
    63121 ccctttaaag cgccatgatg cctgaatgtt tgccagcgta cgggtattga acataccggc
    63181 aaaggcttcc gggtgccagg tgagtgccgc atccacatca tcaaataccc cctcgcggac
    63241 catgaacgtt ttacccgagc cgccttcttc gccaggacaa ccataaaagc gcactgtgcc
    63301 gccttgccca tattgttcca gccatttctt gatggctatt gcagcggcaa aggcggcggt
    63361 tcccagcaaa ttgtgtccgc aaccgtgacc attttcaccg ggcgtcacgg atgtaggttg
    63421 cgcgcaacct gcttgctgac ttaaacctgc cagggcgtca tattctccca gcagggcgat
    63481 aaccggtttg ccttgaccaa acgaagcaat aaaggcattt gggatattgc ctacgttgcg
    63541 ggtaacggtg aagcctgcag attccagcgc cgaagccaga tgctccgctg accagaactc
    63601 ttcaaaacgt gtttctggat gatcccagat ttgatcggca atatcggtat aacgttggcg
    63661 atcggcttca atcgcatcgt cgataaaacg atagatttcc tgcatcagat acctcgcgtc
    63721 cagggaaaat tgagcgcggt gcgcgccagc gtttcgacgg caatagcgag aacctgctcg
    63781 tcaaaatcga atttttcgtt gtgatgacct gccgccagct gtgtgccaaa caccacgtag
    63841 gaggcttgcc cttgatgttg ctgcacgcgg gccatcatta atgtggcatc ttcggaaccc
    63901 gcaggcgctt caacacgttc aatggcctga ttgacccccg cgacctgagc cgcctgactt
    63961 tgcaaccatg cgacccattg cggcgaagga gaactggcgg tagctgcacc catcagacga
    64021 gtttcaacgc cgacaccata catggttgct gcgccctgaa tcgcttgctg agcacggtca
    64081 aaaacatatt gattaatgac gtcgctggcc ccgcgtgttt ccactttcag caacgccgag
    64141 gcaggaacaa cgttacgacc gcttcctgcc tgcataacgc ccacgtttac tctggaagct
    64201 ccttcgctgt gcggggcgat tgcatgcagt gcaagagtgg cttgtgctgc cgccaacaag
    64261 gcattgtaac cgtcttctgg ttttgcgcct gcgtgagcgg cggtaccggt gaagtgcgcg
    64321 tcaaatttgg tggttgccat aaaattatca ctgccgcaca ccacggtgcc cgcaggtacg
    64381 ccagtgccaa tgtgcacggc agtaaaataa tcaacatcat ctacgacacc tgcatcgacc
    64441 atcgcccgcg cgccacgcgt accttcctct gcaggctgaa aaatcagttt gatgacgcca
    64501 tgtagtccgg actcgaattg tttaagggta tgcgccagcc caagcccaat ggtggtatgc
    64561 ccatcatgac cacaggcatg catcattccg gcgttacatg acgcaaaacc gtcgcggtag
    64621 gggcgatggc tgacatcctg ctcttcactg agatccagcg cgtccatatc gacacggaaa
    64681 gccatcaccg gaccggggcg accggtatcc agggtggcga cgatacccgt gaaaccacct
    64741 tcaaaagccg caatccattg tgctagcgca ccctgttgac gagcgcgctc gaattcgcgt
    64801 tgtagagtga gttcatcagg tagtcccatc cggctatttt cattaactac ttcgcggccc
    64861 agcgccagtg agtagccgag ctgctgcaat tcttccgcaa caagggtggc agtgcggaat
    64921 tccacccagc cagactctgc atagtgatga aaatcacgtc gccagtgcga taattttggg
    64981 gcaagcgaat taacaaattg attcaaagac tccataacct ttcccgtcat cagtaaaaag
    65041 tgtgacccgg ttcacgtagc gatagttttt acttatcact aactgatttt tcacagtttt
    65101 aaccgttcat aaattaccct gacacaatca tctgcattaa agtagatgcc agtttctttg
    65161 gtctgataaa taacggttat cggtggcgtc atggcttttc aggtaaaaat tcatcaaatt
    65221 cgggcttttg ttgaagtggc tcgtcagggc agcattcgcg gagcgagccg aatgttgaat
    65281 atgtcgcaac cggcactgag taaatctatt caggagctgg aagaagggtt agcggcgcaa
    65341 ctcttttttc gccgtagtaa aggcgtgacg ttaactgatg ccggtgaaag tttttatcag
    65401 cacgccagtc ttattcttga agaactgcgc gcagcccaag aggatattcg ccaacgacaa
    65461 gggcaactgg cagggcagat taatatcggc atgggggcca gtatttcccg cagtctgatg
    65521 ccagctgtaa tatctcgttt tcatcagcag catccgcagg taaaagtacg cattatggaa
    65581 gggcaactgg tgtcgatgat taatgaattg cgtcagggag aattggattt caccatcaat
    65641 acctattatc agggaccgta cgaccacgaa tttacttttg agaaattact ggaaaagcaa
    65701 ttcgcgatct tttgccgccc gggacacccc gccattggtg cccgttcgat caaacagtta
    65761 ctggattaca gctggacaat gccgacgcca cacggcagct actacaaaca gttgagtgaa
    65821 ttgcttgacg atcaggcgca aacgccacag gtcggtgtag tctgcgagac gttctcagcc
    65881 tgtatcagtc tggtggcaaa aagcgatttt ctcagcatac tgcctgaaga aatgggctgc
    65941 gatcccttgc acggacaggg gctggtgatg ttgccggtta gcgaaatttt accgaaagcg
    66001 gcctattatt tgattcagcg gcgtgatagt cgccagacac cactgaccgc gtcattaatc
    66061 acgcaattcc ggcgagaatg cggctatctg caaagttaaa actgcataaa aaaatagagt
    66121 ctgtcgacat ccgccagact ctacagtaca cacagcagtg catccgcgtc ttaaatccgg
    66181 cattgtctcc tctgcgccgg tgactgtgtt ataacggttt taacctttag ttgccaattt
    66241 tcttcagtga cagatttcac gaaaatataa gttacattat taatatcgtg aatgaataat
    66301 catgcataag cattttgctt aaaatatcgg caatatttgg aacttattac tggaattttg
    66361 ggtaatacgt tgttggaccg acccggtctg gttatcatat cgcgctctta attgcgggag
    66421 gatgtaacat gaaccttgac gacaaatcgc tgtttcttga cgccatggaa gatgtccagc
    66481 cgctgaaacg tgctaccgat gtccactggc atccaacgcg taaccaacgt gcgccgcagc
    66541 gtatcgacac gctgcaactt gataatttcc tcaccaccgg atttctcgac atcatcccgc
    66601 taagtcagcc gctggagttt cggcgggaag ggttgcaaca tggggtgctg gataagctgc
    66661 gcagtggtaa atatccgcaa caggcgagcc tgaatctttt gcgccagccg gtggaagagt
    66721 gccgcaaaat gatgttcagt ttcattcaac aagccatggc ggatggtttg cgtaacgtgc
    66781 tgattattca tggtaaaggg cgggatgata aatcgcatgc caatattgtc cgcagctatg
    66841 tggcgcgctg gttgaccgaa tttgatgatg tccaggcata ttgtaccgcg ctaccgcatc
    66901 atggcggcag cggggcgtgt tacgtcgcac tacgtaaaac ggcacaggca aagcaagaaa
    66961 actgggagcg ccacgctaag cgcagtcgtt gatctcgaga cgcatccgtg gcttatgccg
    67021 ccagcacgcg gttgcgtcca tcatttttcg cccgatacaa agcatcatca acgcgtttaa
    67081 acagttcatc gatgctttca tttccttcgt gatgcgccac accaatgctg acggtaaagc
    67141 gtggtaagcc cgaaatactc acttttgcca cgcttacgcg gatagtttca gccagcgaaa
    67201 gcgcggtatc cagtggggtt cttggtagta ataagacaaa ctcttcgcct ccccaacgaa
    67261 acaccaaatc gcctttgcga gcgcaacttt cgagagtgcg ggcgagggcg cataacacct
    67321 catcaccttt agaatgccca tagagatcgt taatgtgttt aaaacgatct gtgtcgatga
    67381 gcaataagct gtaatcctga gcgatggcga ggtgctgcat ttggcctggt tccgtaatgt
    67441 gataaaactg tcgccgattc agtaatccgg tcatcgcgtc atggtgagca gcatgttcca
    67501 gctgctcctc cagccgtttt tgctcagtaa tatcatgcac aatacataac atgagcttgt
    67561 cgccataaat ttcaatcggt ccggcatagg tctgcacatg acgagtcgaa ccatccgcca
    67621 gcttatgaat aaaattcaga ggtttatgac caccgggtaa atgcgagatt tcatgcatga
    67681 taggcatgac gcgacgcccg agcatattta tttcccaggt atgtttctgg cacatcgttt
    67741 catggttata accatagaaa ttgagcgcgg cgaggttagc atcgacgatt tgtccatctc
    67801 gtgacgggtc aatcaacaac attggtgcag agttagtcag aaaaaagcgc gcataaaaac
    67861 cttgtttttt gcgctgataa tttgccgagc gactggcttt taaacccagc gttgccggcg
    67921 cttcgatacc ttcgaaaata atcaccggtt ctgtttctgt cagctctcgc aaaacaagcc
    67981 gacagcttaa tgctgtttcc tcttctttac gctgaacagt gaggatttcg ataatatcgt
    68041 gttggttttg cagatcggag aggtatttcg gcagttcttt ttgtgaggag acggaatagg
    68101 gaccggttcg tagctgacta aacgtgaggt cttgcatcaa cagtcgcgcc gcgctgttgg
    68161 cataaattaa ctgttcctca aagggcgaaa cgatccagac aggactggtg agtaagtcca
    68221 gagtattgaa gttgtgcgta atcattgaga tcccattatt tttatcaatt tttgttgcta
    68281 tccgatcgca aaaaagccac gtcatatgat cagataattc tgataatgat agacgctatt
    68341 taacacttca cacggtttgt atacggaaaa gcattttgct ttttgtattc aatttagaca
    68401 gaattttatt aatcatttca gggtaatggg gtgatgagat gttgcgtaac agggccagaa
    68461 ggctagacta caaaataatg cgttgatgat ggaggcactg tggaagcgat taagggatcg
    68521 gacgttaatg tcccggatgc agtatttgcc tggatgctgg atggtagagg cggcgttaaa
    68581 ccgctggaaa atacagatgt gattgatgaa gcgcatccct gctggctcca ccttaattat
    68641 gtacaccatg atagcgccca atggctggcg acaacaccgc tgcttcccaa taacgtacgt
    68701 gatgcgctgg cgggcgagag cacacggccc cgagtcagcc gtctcggtga aggcacgctg
    68761 attacattgc gctgtataaa cggcagcacc gatgaacgcc ccgatcaact ggtcgccatg
    68821 cgtgtatata tggacgggcg gttaattgtt tcgacccgac aacgcaaagt gctggcgctg
    68881 gacgatgtgg tgagtgatct ggaagagggc acgggtccga ccgattgcgg gggatggctg
    68941 gtggatgtgt gcgatgcgtt gaccgatcat tccagcgaat ttatcgagca gctgcacgat
    69001 aaaattatcg accttgaaga taatctcctt gatcagcaaa ttccgccgcg tggattcctg
    69061 gctctgctgc gcaaacaatt aattgtgatg cgtcgctata tggcaccgca acgtgatgtt
    69121 tatgctcgtc ttgccagtga acgtttgccg tggatgagcg atgaccaacg ccgtcggatg
    69181 caggatattg ccgatcgcct tgggcgcggc cttgacgaaa tcgacgcctg tatagcacgg
    69241 actggtgtga tggcggatga aatcgctcag gtgatgcagg aaaatttagc tcgtcgtacc
    69301 tatacaatgt cattgatggc aatggtcttt ttacccagta cctttctgac agggttattt
    69361 ggcgtcaacc ttggtgggat ccctggcggc gggtggcaat tcggattttc aattttttgt
    69421 attctgttag ttgttcttat tggtggtgtt gctttatggt tgcatcgtag taaatggttg
    69481 taacaaaagc aatttttccg gctgtctgta tacaaaaacg ccgcaaagtt tgagcgaagt
    69541 caataaactc tctacccatt cagggcaata tctctcttgc aggtgaatgc aacgtcaagc
    69601 gatgggcgtt gcgctccata ttgtcttact tccttttttg aattactgca tagcacaatt
    69661 gattcgtacg acgccgactt tgatgagtcg gctttttttt gcctgttatt tatcagcgtc
    69721 taccctttaa gagtccaccc aatgaccgga gggaaatatg acgacactta tttatttgca
    69781 aattcctgtc cctgaaccga ttcctggcga tcctgttcca gtgcccgatc cgatccctcg
    69841 cccgcaaccc atgcctgacc caccacccga tgaagaaccg attaaattgt cgcatcgtga
    69901 gcgtagatct gcgaggatac gcgcctgatg actttgcgtc gatgaccacg agaatagatt
    69961 gtgaccgctt tttctaccct gaatgttttg cctcccgccc aactcacgaa ccttaatgag
    70021 ttgggttatt taaccatgac gccggtgcag gccgccgcgc ttccggcgat ccttgccgga
    70081 aaagatgttc gcgtgcaggc gaaaactggc agcggcaaaa cggcggcttt tggcctcggc
    70141 ttgttacagc aaattgatgc gtcgctattt caaacccagg ctttagtgct gtgtcctacg
    70201 cgtgaactgg cggatcaggt ggcaggtgaa ttgcgtcggc tggcgcgttt tctgccaaat
    70261 accaaaattt tgacgttgtg cggtggtcaa ccgttcggta tgcagcgtga ttcgttgcaa
    70321 catgcgccgc atattatcgt ggcaacgccg ggccgtttgc tggatcacct gcaaaaaggc
    70381 acggtatcac tggatgcgct gaatactttg gtgatggatg aggccgaccg catgctggat
    70441 atgggattta gcgatgccat tgatgatgtc atccgttttg cgcctgcatc tcgacagacg
    70501 cttctgtttt cggcaacctg gccggaagcc atcgccgcaa tcagcggacg agtgcaacgc
    70561 gatcctttgg cgattgaaat tgactcaaca gatgctttgc cacccattga acaacaattt
    70621 tatgagacat ccagcaaagg caaaattcct ctgttgcaac ggttattaag cttgcatcag
    70681 ccatcctctt gcgtggtgtt ttgcaatacc aaaaaagatt gccaggctgt ctgcgacgcg
    70741 ctgaatgaag tagggcaaag tgcattgtca ttacacggcg atctggagca acgcgatcgt
    70801 gatcagaccc tggtacgttt tgctaacggt agcgcccgtg tactggtcgc gactgatgtt
    70861 gctgcgcgtg gtctggatat taaatcgctt gagctggtgg tgaactttga gctggcgtgg
    70921 gaccctgaag ttcatgtaca tcgcatcggt cgtacagctc gtgcaggaaa tagcggtctg
    70981 gcgatcagtt tctgtgctcc ggaagaagca cagcgggcca atatcatttc tgacatgttg
    71041 cagataaaac ttaactggca aacgccacca gctaatagtt ccattgtgac gctggaagca
    71101 gaaatggcaa cgttgtgtat cgatggcggg aaaaaagcca aaatgcgccc gggtgatgta
    71161 ttaggtgcac tgacaggaga tatcgggctt gatggcgcag atattggcaa aatcgccgtg
    71221 catccggcgc atgtctatgt cgcggtccgt caggctgttg ctcataaagc atggaaacag
    71281 ttacagggcg ggaagattaa aggaaaaacg tgccgggtgc ggttattaaa ataataaaat
    71341 gttgaattgc ctgatgcaaa agtaaacatc ttcgtcggga ttgtcggatg cgacgctgac
    71401 cgcgtcttat tcagcctcca ttagagtagc ccgatacgtt tgcgcatcgg gcgctatccg
    71461 agttatttca cttcaaccac attcagccgt aactcatcca actgattttc atcttcttct
    71521 ggctgccagc ccgccggttg tagtgggatc tcttcgcgat caaacgccag atcacccccg
    71581 ttaaccactt cagaaccgtg ggtgatgcct ttgaaatcga acaggttggt atcgcacaga
    71641 tgcgacggca ccacattctg catcgcgctg aacatcgtct cgatacgccc tggataacgt
    71701 ttatcccagt cacgcaacat gtcagcaatc acctgacgtt gcaggttagg ctgtgaaccg
    71761 cacaggttgc acggaataat cgggaacgct tttgcatcgg caaatcgctg aatatctttc
    71821 tcgcggcagt aggccagcgg acgaataacg atatgtttgc catcatcgct catcagtttc
    71881 ggaggcatac ctttcatctt gccgccgtag aacatattta agaacaacgt ttgcaggata
    71941 tcgtcacgat ggtggcccaa cgcgatcttc gtcgccccca gttccgttgc ggtgcgataa
    72001 aggataccgc gacgtagccg agaacacagt gagcaagtgg ttttgccctc tggaatcttc
    72061 tctttcacga taccgtaagt gttctcttca acaatcttgt actcaacgcc cagcttttca
    72121 agatactcgg gcagaacgtg ttccgggaag cccggttgct tttgatcgag gttaacagcc
    72181 accagcgaaa aattgattgg cgcgctttgc tgcaaattgc gcagaatctc cagcatggta
    72241 tagctgtctt tacccccgga gaggcaaacc atgatgcgat cgccttcttc aatcatattg
    72301 aagtcagcaa tggcttcgcc cacgttacga cgcaggcgtt tttgtaattt gttcaggttg
    72361 tattgttctt tcttgctaat ttcttgattt tgcgacattt aaaagcgact caattcgtta
    72421 tatggcatca gaagagtatg cgtcatgccg gaacgcccag cataagaaat ctgatataaa
    72481 aactgtggcg tgtatggtac ggattagagg ggaaaatgtc agcacatttg cgaaatgaat
    72541 caaaaagccc gcagcaatgt gcgggcgtta gtgtcagcgc acaaccagca cggagcactc
    72601 tgcgtgacgc actacagctg cggcgttgga accgagcaga taagtggtga tatccggtcg
    72661 atgggaagca atgatgatca tatgagcggg gatcttcttc gccaattcca gaatgcggtc
    72721 tttgggcgag ccttcctcaa catggacatg cactctgtcg gttggcagtt taaatttttt
    72781 aatgatctct tccagttgcg atttggcttc cgctttcagg tcatccattg ccggtaattc
    72841 tgcggaatac gctaaaccca gagaggcata gtagggcagt gaaggtatta ccgtcaggaa
    72901 atgaacctct gcatcatcaa tctttgcctc tgcctcaacg tggctaatca cgcgttgagt
    72961 taattctgaa tcggaaatat cgatagggac aagaatcgtt ctgttcataa aacctcctgt
    73021 tttagtatcc gcataaagtg taacgccaga tgacactttt tgtgtaatga cggagttcac
    73081 atttttaatt tagatcaaag gaggaagaat aagcagaaaa agcccgccat aacagcgggc
    73141 aggaggattt agaactgata aaccagacct aaagcgacaa tatcatcggt agagatgccg
    73201 ttggcagcgt agaaactgtc atcttcatcc aacaggttga ttttatagtc aacataagtg
    73261 gacatgtttt tattgaaata gtaagtcgcg ccaacatcgg cgtatttaac cagatcttta
    73321 tcatcaacac ctgccgggtt gtctgcacca ccggcagcgt gcaggtcacg gcctttagac
    73381 atcaggaaag agactgccgg acgcagacca aaatcaaact ggtactgtgc agtgacttca
    73441 aagttctggg ttttgtttgc aacagcataa tcgctgtcgc caaacggggt catattacgc
    73501 gtttctgaat acatggttgc caggtaaata ttgttagcat cgtattttag cccagcagtc
    73561 cacgcgtctg ctttatcacc acccgccgca gtatggttaa cctggtcatt ggtgcggtca
    73621 gaagaggtgt atgccgcacc agcgctaaag cccatgccta aatcatatgt tgtggaaaga
    73681 ccccagccgt caccgttttc atggcgaaca tcacgtccgt tgttggtgcc ttcctgacca
    73741 ttactggcac cttcgttgtt accttgatac tgcaccgcga agttcagacc atttaccaga
    73801 ccgaagaaat cagtattacg ataagtcgcg acgccattgg cacgaccagt cataaagttg
    73861 tctgcattgg tataagagtc accgccaaat tcaggcagca tatcggtcca gcccataatt
    73921 acgtccgtaa tcgaaagaac cgtaatctga aaatttcagt ccggcaaatg ccagacgggt
    73981 ccatgactgg ttttttgaag attcagtgtt gtttgcctga atattgtatt cccattgacc
    74041 gtagccagta agttgatcgt taatttgggt ttcgccttta aaacccagac gcgcatagct
    74101 ctggtcgcca tctttcgctg aattatcaga aaaataatgc aggccatcaa ctttgccata
    74161 cagatctaat ttgttgccgt ctttattata aacttcggct gcatgtgcag cacctgcgcc
    74221 gagcagggca ggaattaaaa gtgccagtac tttgcttttc attgaataaa tcctttagtt
    74281 attttatttg ccttttatcc ccatttcggg gagtgaaaac atcctaaagg agaagttcag
    74341 ttagccgata aatatcattt gttacgcctt aagtaaaacc ttaatcaaat
//
LOCUS       DAZFDC010000009        67523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFDC010000009 DAZFDC010000000
VERSION     DAZFDC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.009"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..632
                     /locus_tag="ACHJ48_000698"
                     /pseudo
     CDS             1..632
                     /locus_tag="ACHJ48_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            735..1238
                     /locus_tag="ACHJ48_000699"
     CDS             735..1238
                     /locus_tag="ACHJ48_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO4706314.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1305..2762)
                     /gene="nuoN"
                     /locus_tag="ACHJ48_000700"
     CDS             complement(1305..2762)
                     /gene="nuoN"
                     /locus_tag="ACHJ48_000700"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO4706315.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2769..4298)
                     /gene="nuoM"
                     /locus_tag="ACHJ48_000701"
     CDS             complement(2769..4298)
                     /gene="nuoM"
                     /locus_tag="ACHJ48_000701"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO4706316.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4529..6370)
                     /gene="nuoL"
                     /locus_tag="ACHJ48_000702"
     CDS             complement(4529..6370)
                     /gene="nuoL"
                     /locus_tag="ACHJ48_000702"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO4706317.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6367..6669)
                     /gene="nuoK"
                     /locus_tag="ACHJ48_000703"
     CDS             complement(6367..6669)
                     /gene="nuoK"
                     /locus_tag="ACHJ48_000703"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO4706318.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6666..7220)
                     /gene="nuoJ"
                     /locus_tag="ACHJ48_000704"
     CDS             complement(6666..7220)
                     /gene="nuoJ"
                     /locus_tag="ACHJ48_000704"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO4706319.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7232..7774)
                     /gene="nuoI"
                     /locus_tag="ACHJ48_000705"
     CDS             complement(7232..7774)
                     /gene="nuoI"
                     /locus_tag="ACHJ48_000705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO4706320.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7789..8766)
                     /gene="nuoH"
                     /locus_tag="ACHJ48_000706"
     CDS             complement(7789..8766)
                     /gene="nuoH"
                     /locus_tag="ACHJ48_000706"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO4706321.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8763..11489)
                     /gene="nuoG"
                     /locus_tag="ACHJ48_000707"
     CDS             complement(8763..11489)
                     /gene="nuoG"
                     /locus_tag="ACHJ48_000707"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO4706322.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQCRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11542..12879)
                     /gene="nuoF"
                     /locus_tag="ACHJ48_000708"
     CDS             complement(11542..12879)
                     /gene="nuoF"
                     /locus_tag="ACHJ48_000708"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO4706323.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12876..13376)
                     /gene="nuoE"
                     /locus_tag="ACHJ48_000709"
     CDS             complement(12876..13376)
                     /gene="nuoE"
                     /locus_tag="ACHJ48_000709"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO4706324.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHANLTPEAIPE
                     LLERYK"
     gene            complement(13379..15181)
                     /gene="nuoC"
                     /locus_tag="ACHJ48_000710"
     CDS             complement(13379..15181)
                     /gene="nuoC"
                     /locus_tag="ACHJ48_000710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311197.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO4706325.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPARVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15275..15937)
                     /gene="nuoB"
                     /locus_tag="ACHJ48_000711"
     CDS             complement(15275..15937)
                     /gene="nuoB"
                     /locus_tag="ACHJ48_000711"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO4706326.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15953..16396)
                     /gene="nuoA"
                     /locus_tag="ACHJ48_000712"
     CDS             complement(15953..16396)
                     /gene="nuoA"
                     /locus_tag="ACHJ48_000712"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO4706327.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17027..17965)
                     /gene="lrhA"
                     /locus_tag="ACHJ48_000713"
     CDS             complement(17027..17965)
                     /gene="lrhA"
                     /locus_tag="ACHJ48_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO4706328.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
     gene            18885..20102
                     /gene="alaA"
                     /locus_tag="ACHJ48_000714"
     CDS             18885..20102
                     /gene="alaA"
                     /locus_tag="ACHJ48_000714"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO4706329.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPCGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20186..20785
                     /gene="yfbR"
                     /locus_tag="ACHJ48_000715"
     CDS             20186..20785
                     /gene="yfbR"
                     /locus_tag="ACHJ48_000715"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4706330.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20844..22676)
                     /locus_tag="ACHJ48_000716"
     CDS             complement(20844..22676)
                     /locus_tag="ACHJ48_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO4706331.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22763..23413)
                     /gene="hxpA"
                     /locus_tag="ACHJ48_000717"
     CDS             complement(22763..23413)
                     /gene="hxpA"
                     /locus_tag="ACHJ48_000717"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO4706332.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23424..23918)
                     /locus_tag="ACHJ48_000718"
     CDS             complement(23424..23918)
                     /locus_tag="ACHJ48_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO4706333.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24001..24456)
                     /gene="yfbV"
                     /locus_tag="ACHJ48_000719"
     CDS             complement(24001..24456)
                     /gene="yfbV"
                     /locus_tag="ACHJ48_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO4706334.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24794..25996
                     /gene="ackA"
                     /locus_tag="ACHJ48_000720"
     CDS             24794..25996
                     /gene="ackA"
                     /locus_tag="ACHJ48_000720"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO4706335.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26071..28215
                     /gene="pta"
                     /locus_tag="ACHJ48_000721"
     CDS             26071..28215
                     /gene="pta"
                     /locus_tag="ACHJ48_000721"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO4706336.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28405..29925
                     /gene="yfcC"
                     /locus_tag="ACHJ48_000722"
     CDS             28405..29925
                     /gene="yfcC"
                     /locus_tag="ACHJ48_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO4706337.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRLLTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29958..30500)
                     /gene="yfcD"
                     /locus_tag="ACHJ48_000723"
     CDS             complement(29958..30500)
                     /gene="yfcD"
                     /locus_tag="ACHJ48_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO4706338.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30558..31109)
                     /gene="yfcE"
                     /locus_tag="ACHJ48_000724"
     CDS             complement(30558..31109)
                     /gene="yfcE"
                     /locus_tag="ACHJ48_000724"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO4706339.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31165..31809)
                     /gene="yfcF"
                     /locus_tag="ACHJ48_000725"
     CDS             complement(31165..31809)
                     /gene="yfcF"
                     /locus_tag="ACHJ48_000725"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO4706340.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLLFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31945..32592
                     /gene="yfcG"
                     /locus_tag="ACHJ48_000726"
     CDS             31945..32592
                     /gene="yfcG"
                     /locus_tag="ACHJ48_000726"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO4706341.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     LISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32649..33011
                     /gene="folX"
                     /locus_tag="ACHJ48_000727"
     CDS             32649..33011
                     /gene="folX"
                     /locus_tag="ACHJ48_000727"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO4706342.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33032..33925
                     /locus_tag="ACHJ48_000728"
     CDS             33032..33925
                     /locus_tag="ACHJ48_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO4706343.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGIDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNKFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33973..34863)
                     /gene="rpnB"
                     /locus_tag="ACHJ48_000729"
     CDS             complement(33973..34863)
                     /gene="rpnB"
                     /locus_tag="ACHJ48_000729"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO4706344.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYLYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDVGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(35060..35833)
                     /gene="hisP"
                     /locus_tag="ACHJ48_000730"
     CDS             complement(35060..35833)
                     /gene="hisP"
                     /locus_tag="ACHJ48_000730"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO4706345.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35841..36557)
                     /locus_tag="ACHJ48_000731"
     CDS             complement(35841..36557)
                     /locus_tag="ACHJ48_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4706346.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36554..37240)
                     /gene="hisQ"
                     /locus_tag="ACHJ48_000732"
     CDS             complement(36554..37240)
                     /gene="hisQ"
                     /locus_tag="ACHJ48_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO4706347.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAARAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37330..38112)
                     /gene="hisJ"
                     /locus_tag="ACHJ48_000733"
     CDS             complement(37330..38112)
                     /gene="hisJ"
                     /locus_tag="ACHJ48_000733"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO4706348.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38333..39115)
                     /gene="argT"
                     /locus_tag="ACHJ48_000734"
     CDS             complement(38333..39115)
                     /gene="argT"
                     /locus_tag="ACHJ48_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO4706349.1"
                     /translation="MKKSILALSLLVGISAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39381..39950)
                     /gene="ubiX"
                     /locus_tag="ACHJ48_000735"
     CDS             complement(39381..39950)
                     /gene="ubiX"
                     /locus_tag="ACHJ48_000735"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO4706350.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40045..41562)
                     /gene="purF"
                     /locus_tag="ACHJ48_000736"
     CDS             complement(40045..41562)
                     /gene="purF"
                     /locus_tag="ACHJ48_000736"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO4706351.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41599..42087)
                     /gene="cvpA"
                     /locus_tag="ACHJ48_000737"
     CDS             complement(41599..42087)
                     /gene="cvpA"
                     /locus_tag="ACHJ48_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO4706352.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGLLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42619..43281)
                     /gene="dedD"
                     /locus_tag="ACHJ48_000738"
     CDS             complement(42619..43281)
                     /gene="dedD"
                     /locus_tag="ACHJ48_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO4706353.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43271..44539)
                     /gene="folC"
                     /locus_tag="ACHJ48_000739"
     CDS             complement(43271..44539)
                     /gene="folC"
                     /locus_tag="ACHJ48_000739"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO4706354.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVCYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44609..45523)
                     /gene="accD"
                     /locus_tag="ACHJ48_000740"
     CDS             complement(44609..45523)
                     /gene="accD"
                     /locus_tag="ACHJ48_000740"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO4706355.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45679..46338)
                     /locus_tag="ACHJ48_000741"
     CDS             complement(45679..46338)
                     /locus_tag="ACHJ48_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4706356.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46421..47233)
                     /gene="truA"
                     /locus_tag="ACHJ48_000742"
     CDS             complement(46421..47233)
                     /gene="truA"
                     /locus_tag="ACHJ48_000742"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO4706357.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47233..48246)
                     /locus_tag="ACHJ48_000743"
     CDS             complement(47233..48246)
                     /locus_tag="ACHJ48_000743"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO4706358.1"
                     /translation="MSEGWNIAVLGTTGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48312..49448)
                     /gene="pdxB"
                     /locus_tag="ACHJ48_000744"
     CDS             complement(48312..49448)
                     /gene="pdxB"
                     /locus_tag="ACHJ48_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO4706359.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADCGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49547..50542
                     /gene="flk"
                     /locus_tag="ACHJ48_000745"
     CDS             49547..50542
                     /gene="flk"
                     /locus_tag="ACHJ48_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO4706360.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHCPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50539..51717)
                     /locus_tag="ACHJ48_000746"
     CDS             complement(50539..51717)
                     /locus_tag="ACHJ48_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4706361.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51992..53212)
                     /gene="fabB"
                     /locus_tag="ACHJ48_000747"
     CDS             complement(51992..53212)
                     /gene="fabB"
                     /locus_tag="ACHJ48_000747"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO4706362.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53371..55377
                     /gene="mnmC"
                     /locus_tag="ACHJ48_000748"
     CDS             53371..55377
                     /gene="mnmC"
                     /locus_tag="ACHJ48_000748"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO4706363.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     ISQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNGFTFAR
                     RLYDSLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLTFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEDNQQQNRQRLIDCFPHAQWAKTVDVSKKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55498..55776)
                     /locus_tag="ACHJ48_000749"
     CDS             complement(55498..55776)
                     /locus_tag="ACHJ48_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO4706364.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55810..56358)
                     /gene="epmC"
                     /locus_tag="ACHJ48_000750"
     CDS             complement(55810..56358)
                     /gene="epmC"
                     /locus_tag="ACHJ48_000750"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO4706365.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56358..57167)
                     /locus_tag="ACHJ48_000751"
     CDS             complement(56358..57167)
                     /locus_tag="ACHJ48_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO4706366.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57167..57991)
                     /gene="mepA"
                     /locus_tag="ACHJ48_000752"
     CDS             complement(57167..57991)
                     /gene="mepA"
                     /locus_tag="ACHJ48_000752"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO4706367.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRRTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57995..59080)
                     /gene="aroC"
                     /locus_tag="ACHJ48_000753"
     CDS             complement(57995..59080)
                     /gene="aroC"
                     /locus_tag="ACHJ48_000753"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO4706368.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59115..60047)
                     /gene="prmB"
                     /locus_tag="ACHJ48_000754"
     CDS             complement(59115..60047)
                     /gene="prmB"
                     /locus_tag="ACHJ48_000754"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4706369.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKAQLLAAREHFAIYKD"
     gene            60213..60764
                     /gene="smrB"
                     /locus_tag="ACHJ48_000755"
     CDS             60213..60764
                     /gene="smrB"
                     /locus_tag="ACHJ48_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO4706370.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQVDASHYFSDEFQPLLNADGPVKYVRPGVDHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60935..61777)
                     /locus_tag="ACHJ48_000756"
     CDS             complement(60935..61777)
                     /locus_tag="ACHJ48_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO4706371.1"
                     /translation="MKKWTIFLTSLILLALSLETPKCYAGDKLMSASFSSTKIYYAMK
                     NVTASGSLYFYVTVVTPGEVSYGQYNSNARKGDTLKLISWSGSGPAPTLVLTDYRRTD
                     TSNCPGINTRVFVCAYMTFNVTVESDNYGCPWIASFYSVSEAFGFGTYTSPTVHDSIC
                     PTIPVASFDISWSDNYVSHNKALRLQSDGSTITTTLSTYLMESGKLCDGSIFDSRGAY
                     CRAVSDLLTFTSYGCDNAKVTVTPSRQPLTDRKLHDIVVQVNTSSREPIDSTCRFQYV
                     LNEL"
     gene            complement(61779..62300)
                     /locus_tag="ACHJ48_000757"
     CDS             complement(61779..62300)
                     /locus_tag="ACHJ48_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4706372.1"
                     /translation="MKNNRAWALISGLILFSGTAPAADNLHFTGNLLGKSCTPVINGN
                     LLAEIHFPTIAASDLMQRGQSDRVPLVFQLKDCKSTTAFNVKVTLMGTEDTDLPGFLS
                     IDSSSSATGVGIGIETAGGAAVPINSTTGASFPLNQGNNSVNFNAWLQTVNGRNVTSG
                     DFTATMTVTFEYF"
     gene            complement(62297..62767)
                     /locus_tag="ACHJ48_000758"
     CDS             complement(62297..62767)
                     /locus_tag="ACHJ48_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4706373.1"
                     /translation="MKRISLLMLWSFSFMALSNVSFHGYLVQPPNCSISNGQNIELTF
                     RDVNIDDINGSNYEQVVPYTITCDTAVRDPQMEMTLTWSGTQSDFDDSAVATDLNGLG
                     IHLKQAGSDFKLHTPLVVNETSLPVLTAVPVKKSGVDLPESDFEAWATLQVDYQ"
     gene            complement(62764..63264)
                     /locus_tag="ACHJ48_000759"
     CDS             complement(62764..63264)
                     /locus_tag="ACHJ48_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4706374.1"
                     /translation="MKGAYSAWAWLLLCSIVSTPVMAGSKSVAMVLRVLVDAPPPCTV
                     TGASVEFGNVFISKIDGVSYKRPIDYSLVCNNLAMDDLRLNMQATTTVINGETVIDTG
                     IAGFGIRIQKVSDHSILDLTPGAWLPFNFSSGALALEAVPVVQSGVSLTAAEFSASAT
                     IVVDYQ"
     gene            complement(63275..64033)
                     /locus_tag="ACHJ48_000760"
     CDS             complement(63275..64033)
                     /locus_tag="ACHJ48_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4706375.1"
                     /translation="MTNLFCSARTAATTLALLITAASLSVQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKMPYLAQSWMEDGQGNRISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKV
                     TLTLSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPV
                     LTYVNDYGARLPLFFRCTGNTCQVDEEQNSKGQG"
     gene            complement(64056..66695)
                     /locus_tag="ACHJ48_000761"
     CDS             complement(64056..66695)
                     /locus_tag="ACHJ48_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4706376.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAENEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     LHSKSNDDDVINGDDTQKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQLGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GSLDGNSFRLSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSISMTGYR
                     YEYDNQADKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSYPPFGAEVKNDSAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISPSMPHEI
                     KPVIQEQTQQVMPTEAPVSVSAN"
     gene            complement(66777..67340)
                     /locus_tag="ACHJ48_000762"
     CDS             complement(66777..67340)
                     /locus_tag="ACHJ48_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4706377.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16677 a        18335 c        16952 g        15559 t
ORIGIN      
        1 attttccttt tgttatatta tttctgtttt cactccctct gattattttt taccaaccct
       61 gggtcactgc attatcgcca acgccccgac acgtaattcc tgagcaatta gaaaaaacag
      121 ttcgttatct tacacaaact gtgcatccac gtaatgccga caatattgat aatctgaata
      181 ggtccgtaga gtatataaaa aaaggtcttt gtcagtagcg gtgccagagt taccgcgcag
      241 ggcgtcccca attggctatt acgactctgc gcctggtagc caggattacc cttacccgca
      301 atgtcctggc tttatcccga tcggggggac tttattgcct ggtcggcaga atacaggata
      361 tcaacgccgt tcgtcaggta aaaacggcat tgttatcatc ccgggattta tctgtttatt
      421 ctatgaatgc cccatggttt attcacggta ttgatttctc tgaccacctt aattattggc
      481 aacacgatat tctcgccgta atgattactg acaccgcttt ttatcgtaat aaacaatacc
      541 acttgcccgg tgatacagca gacagattga attatcagaa aatggctcag atggtggatg
      601 gtgttataac tttgttacac aacagtaaat aataaaaatt tctcgaggcg ggtccagaaa
      661 cagcgacatg tacattttat tttccctcgt ctgttttaag atgcgaaaca taaaattcaa
      721 tggaggaaaa ataaatggga atgattggtt attttgcaga aattgactca gaaaagatta
      781 accaactgct tgagtctact gaaaaacctt tgatggataa tatacatgac acgctttcag
      841 gattacgccg actggatatt gataaacgct gggatttttt acactttggg ctgacaggca
      901 cctctgcgtt cgatccagcg aagaatgatc cgctgagtcg tgccgtgctt ggtgaacaca
      961 gcctcgaaga tggtatagat ggtttcctgg gattgacatg gaatcaggag ttagccgcga
     1021 caatcgaccg actggaatca ctggatcgaa gtgagctgcg taaaaagttt tccattaagc
     1081 gactcaatga aatggagatt tatccgggag tgaccttttc agaagagtta gaggggcaac
     1141 tctttgcttc gatcatgctt gatatggaaa agctgattag cgcgtatcga cgtatgctgc
     1201 gacaagggaa tcatgcgctg acggttattg ttgggtgatg gtgtcagggc accaaaaaaa
     1261 accgccgaac atgtcggcgg ctttctgact tacaaactga tagcttacat cagcggcatt
     1321 gctaaccgca caatactaat cagcggttgt ggccatacac ccagcaccag taccaacagt
     1381 gcagaaatca gcaccacgat accgcccgcg ctgtactgcc agtttgacgg tgcatcgcga
     1441 cccggttgtt ccggtgcgtg caggtacagg ctcaccgcca cgcgcaggta gtagtagagg
     1501 ccgattgccg aaccgacaac cacggcaccc accagccacc acaggtgtgc ctggacacca
     1561 accgccagca cgtagaactt accgataaag cccagcgtca tcgggatacc ggccagagac
     1621 agcatcatca ccgtcatcac cgccgcgagg atcggacgat gccagaacag accgcggtaa
     1681 gagaacaggg aatcagcatc cgggccacga tacgggctgg acatcaggct gaccacgccg
     1741 aacgcgccga ggctgctgaa cagataaccg gccaggtaaa cccctaccgc ttccatcgac
     1801 atctcgccag tttgcagcgc aatcagcgcc accagcagat agccgaggtg agagatagat
     1861 gagtaaccga gcagacgttt gatattggtc tggctcagcg ccatcaggtt accgaagatg
     1921 atggaggcaa aggcgataat cgccagcacc acccgaatcg cttcgctgtc acccaccggt
     1981 gcgtagagga acagacgcat caccacaccg aagatagcga ttttgctcgc cgtcgccagg
     2041 aaagtggaaa ccggcgcagg cgcgccctgg tatacgtctg gcgtccacag gtggaacggc
     2101 accagagaga gtttgaagcc gaggccaaca atcatcaggc cgaaacctgc cagcaacagc
     2161 ggctcgttta gcataccgtc gccgaggttt ttacccagcg cgacaaacga caggtcgcca
     2221 gactgcgcat acaccagcgc cataccgaac agcaggaaag aagacgctgc ggcagaaagg
     2281 atggtgtatt tgatactggc ttccagcgaa cgtttctggc ggaaagcgta accgaccagg
     2341 ccaaacagcg gcaaagagat cagttcaata ccgaggaaca gagacgccag atggtttgca
     2401 ttcgccagca ggatcccgcc cagcgcggca attaacacca acaggtagaa ctcatccttg
     2461 ttgtcgttat agccttcaag ccacgggtag gcgaaagtac aggtggcgag gctcgccaac
     2521 aataccagcc cggtgtaaag catggcgaaa ccatcaacgc gcatcagcgg cgtaacgtcc
     2581 atagcgcccg cctggccaac aaaccagagc gaaaccagcg ccgcgttaag cccaataacc
     2641 gagagcgtag cgttgaggaa atgattgcgt cgccacgcaa tggagagcat cacaaccacc
     2701 accgtcaagc cgacgatcag caacggtagc agtgcgatca ggttttgtgg agttattgtc
     2761 atggcgattt acggccttgt agtagtaacg gaattaacaa accactgctg gatattgcca
     2821 atcgcggagt gcgaggtatc cagaatcggc tgcggataga agcccagcag taccagcagc
     2881 accaccagca acaggatcat aaacagctca cgcagcgaca tccctggcag ttcctggctg
     2941 gcaatctggc ttttcgcttt accgaagtaa gcgcgatgta acatcgccag cgaataaaca
     3001 gatgcaaaga ccagcccaaa ggtagagata acggtaatca ccgggacaac ctggaagctg
     3061 ccgaacagaa tcataaattc gccgacgaag ttaccggtgc ctggcatccc gagcgttgcc
     3121 accgcaaaga acagtgacaa ggctggcagc catttcatct tgctccacag accgcccatc
     3181 atgcgcatgt cgcgggtatg gatacgttca taaagctgac cacaaagaat aaacagaccc
     3241 gccgctgaca agccgtgcgc aatcatctgg attaccgcgc cctggtaggc caactggctg
     3301 ccggtgtaga tagcaatcag tacgaagccc atgtgggaaa ccgaggtgta ggcgatcaga
     3361 cgtttgatat cggtctgggc gaaggccatc cacgcaccgt agaagatgcc gataacaccc
     3421 agccacatgg cgattggcgc gaactctgcc gacgcgtttg ggaacagcgg tagggagaaa
     3481 cgcagcagac cgtaagcggc ggttttcagc aagatccccg cgaggtccac ggaaccggcg
     3541 gtcggtgcct gtgagtgcgc atccggcagc cagccatgca gcggaaccac cggcattttc
     3601 accgcaaacg cgatgaagaa gcccagcatc aacagatatt ccacaccact ggacattggc
     3661 gtattcagca gctcttcata gttgaaggtc cagacgccgg tcgcattgta gtgaacaaaa
     3721 accagcgcca ggatggcgat caacatcacc agaccactcg cctgggtgta aatgaagaac
     3781 ttggttgccg ccgtgatacg cgttttacca tcagaggctt tatgccccca cagtgcgatc
     3841 aggaagtaca tcggcaccag catcatttcc cagaagaaga agaacaggaa catgtcgatg
     3901 gcaaggaaca cgccgataac gccgcccagg atccacatca ggttgaggtg gaagaagccc
     3961 tgatattttt cgatctcttt ccacgaacag agtaccgcca gcacaccgag cagaccggtc
     4021 agcacgacca tcagcagcga cagcccgtca atggcgagat ggatagagat accaaaacgc
     4081 gggatccacg gcatgtcgaa ttcagactgc cactgcggaa ttccggcgga ttgcgtcagt
     4141 gaataaccgc cctgcaacca cagttgcagc gacagcgcca gcgtcaatcc catggtgatc
     4201 agcgcgatcc agcgcggcac cttgacgcca aagcgttcgg tctgccagca caggaagccg
     4261 ccgataaagg ggattagtat taaccagggt aataacatag ctaagtatgt cccttatatt
     4321 tactcctgct caggctatct cactcgccag cccgaatccc ggcagtgctc gcaatcctca
     4381 cgtacttaag tacgctccgg ttgctgtgcg ctggcggtat ccggtctgac ttcgccgatg
     4441 acgcctgtac ttcaggaaca gttacctaaa atttattctt agttcacaga cccggcgcaa
     4501 cgccgggtat attcactcag ccaacaactc aacgcagtac catcaacagt gccagcacca
     4561 cgaccgcacc gatgctcatg gatgccacat accagcgcag ataaccgttc tcgcttaaca
     4621 gcaggccttt acctgcaaag cgggaaagga cagccgggat gttcatcatt gagttcagcg
     4681 gatcgcgttt cagcaaccag gcaataccca ggaacggctt gacgaacact ttgtcgtaca
     4741 gccagtcaaa gccccaggcg ttgtaccacc aggtgcccag cagacggccc ggcgcactgt
     4801 tggcgatgga ggtcaccaga gtacgtttac ccagccacag ccaggctgcc agcagaatgc
     4861 cgaccaccgc gaccacgcca gaggtaattt ccagggtcaa catgctgccg tgcgccagtt
     4921 ccgtcgtttg cggaagcacg ccctgcagcg gcggtacaat cagtgcgcca acgaaggtgg
     4981 aaaggatcag cagcacaatc agcggcaggc tgtgagttac ccctttcacg gcgtgagcgt
     5041 gaatttgttc ttttccgtgg aagacgatga aaatcatacg gaaggtgtag agcgaggtca
     5101 taaacgcacc gaccagacct gccaccatca gattgatatg accattcgcc atcgcacccg
     5161 cgaggatctc atccttactg aagaagcccg cagtgactag cggcagtgcc gacagtgctg
     5221 cgccgcccac caggaagcag agataaacca gcggaataga tttacgcaga ccgcccatct
     5281 tgaagatgtt ctgttcgtga tggcaggcga gaatgacgga accggatgcc aggaacagca
     5341 gcgctttaaa gaacgcgtgg gtcatcaagt gaaaaatcgc cgcatcccat gcctgcacgc
     5401 caagcgcgag gaacatgtag ccaatctggc tcatggtaga gtaagcgaga acacgtttga
     5461 tgtcggtctg taccagcgcg gcaaaaccgg ccagcagcag cgtaaccgcc ccaacaatgc
     5521 ccaccagatg cagaacttcc ggcgtcatca ggaacaggcc gtgggtacgg gcgatcaggt
     5581 agacacccgc ggttaccatc gttgcggcgt ggatcagcgc ggagacaggc gtcgggcccg
     5641 ccatcgcgtc ggcaagccat gtctgcaacg gcaactgcgc agatttaccg accgcaccgc
     5701 ccagcagcat cagcgtcgcc cacatcagca tgttattgcc gtcagcaaag tgcgctggtg
     5761 ccagttccac catttcgcgg aagttcaggg tgcccagttc gttgtaaaga atgaacagcg
     5821 cgaaagcgag gaacacgtca cccacacggg tcacgacgaa cgctttcatt gccgctgcgc
     5881 cattcttcgg atcggtgtaa tagaacccga tcagcagata ggagcacagg cccacgcctt
     5941 cccagccgag gtacatcagc agcaggttgt cggcaagcac cagaaccacc atgctggcga
     6001 taaacaggtt ggtgtaagcg aagaagcgag agtagccctc ttcaccgcgc atataccagg
     6061 aggcgtacat gtgaataagg aaacccacac cagtgaccac cgagagcatg gtcagcgaca
     6121 ggccgtccag caccaggtta aaaccgatgt taaagtcgcc taccgacatc cacgtccaca
     6181 gcggctggct gtatgcctgc tcgccgttag cgaagaagtc aacgccaata aaggcagtta
     6241 ccagcgccgc caggcccaca gagccaacgc cgacgatcgc cgagacgttt tcagaccagc
     6301 gcccacggga gaacgccagc aggacgaagc caatcaatgg caaaataatg gttaaggcaa
     6361 gcatgttcat ccgcgcatct cacttactga atcgatgttc aggttctggc gacgacggtg
     6421 aagttgcagc agcagcgcaa ggccgatact cgcttctgcc gccgcgaggc tgatggcgag
     6481 aatgtacatc acctgaccgt cggtctggcc ccagtaactt ccggcgacca caaaggccag
     6541 cgcggaagcg ttaatcatga tttccagacc aatcagcata aacagcagat tgcgacggat
     6601 aaccagaccg gttaagccaa gaacgaataa gattgccgcg aggatcagtc catgttgtaa
     6661 ggggatcatg cgtgctcctc cgtttttctt ttcgcgctgt cgtctttacg attgctcagc
     6721 acttcacccg cacgctcttc acgaccgacg tggaaggcca caaccagacc cgcgagcagc
     6781 agcatagaag ccagttccac cgccagtacg taaggcccga acagcgtaat acccactgct
     6841 ttagcactga ttggcgtacc gtcgatacct tgatcgttaa caccgagaat ggcgtaaaca
     6901 atcaccacca acatgatggc tgacaaaatt gccggaccaa tccacacctg cggtttcagc
     6961 cactggcgtt cttgttcgat ttctgaaccg cccaggttga gcatcatcac cacgaacacg
     7021 aacagcacca taatggcacc cgcgtagacg ataatttcca gcgcaccggc gaagtaagcg
     7081 cctagtgaga agaacacccc ggaaatcgcc agcagcgaaa taatcaggta cagcagtgcg
     7141 tgtaccggat tggtatgggt gatcactcgc aaggtcgcaa gtatggctat caggccacag
     7201 atataaaaag cgaactccat tgcccctctc cttacggtaa caggctcttg acgtcgatag
     7261 gcttggcttc gttctctgct tcgcccttat ctttgccgtc gattgccata cctgccatcc
     7321 ggtagaagtt atactccggg tatttgcccg gaccggagat cagcagatcc tctttctcgt
     7381 aaaccagatc ctggcgcttg tattccccca tttcgaaatc cggggttaac tgaatcgccg
     7441 tggtcggaca ggcttcttcg cacaaaccac agaaaatgca gcgtgagaag ttgatgcgga
     7501 aaaattccgg ataccagcga ccgtctttgg tttctgcttt ttgcagcgag atacagccga
     7561 ccgggcaggc taccgcgcag aggttacagg ctacgcaacg ctcttcgccg tccgggtcgc
     7621 gggtcagaac gatacgacca cgataacggg gcggcagata gaccggctct tccgggtaca
     7681 ttcgcgtttc gcgtttggcg aacgcgtgca ggccgatcat ccagatacta cgaacctggg
     7741 tgccgaaacc tactaacaat tcttttaagg tcatggtctt attgcccctt attgcgcctg
     7801 ccagagaatg acagccgccg ttaccagcaa gttgatcagc gtcagcggca ggcagatttt
     7861 ccagccgaag gacattacct ggtcataacg cggacgcggt aacgacgcac gaatcaaaat
     7921 gaacatcatc ataaagaacg cggttttcag cgcgaaccag atgaatggcg gtaacaacgg
     7981 gccttgccag ccaccgaaga acagcgtcac catcaatgca gagatggtca caatcccgat
     8041 gtactcaccc acgaagaaca gaccgaactt cataccggaa tattcaatgt ggtaaccatc
     8101 cgccagttcc tgctcggctt ccggctggtc aaacgggtga cggtgacaca ccgccacgcc
     8161 cgcgatggca aaggtaataa aaccaaagaa ttgcgggatg acgttccaca catgcgcctg
     8221 gctgttgacg atgtcggtca tgttgaatga accggcctgc gccaccacgc ccatcaggga
     8281 aagcccgagg aacacttcgt agctcagggt ctgcgcagaa gcacgcatcg caccgagcag
     8341 cgagtatttg ttgttacttg accagcccgc aaacagcacc gcgtaaaccg ccagaccggc
     8401 catcatcagg aagaacaaaa tcccgatgtt caggtcggca accacccaac ccggactgac
     8461 tggcacaatc gcaaaggcca gcagcagcga ggtaaaggca atcatcggtg ccagggtaaa
     8521 gatgacgcga tccgagaatt tcgggatcca gtcttcttta aagaacattt tgatcatgtc
     8581 cgcaaccagc tggagcgaac caccccagcc aacacggtta ggtccgtaac ggttctggaa
     8641 cagacccagc aggcgacgtt cgccaaagct catgaatgcc ccgcaggtga caaccaccag
     8701 caggatcacc accgctttga ggatggtcag caggatctca atcagttccg gtgatatcca
     8761 actcattgtt gtgcctcctt gagatcctca agatgcgcgc cagccagcac cggagcaatg
     8821 ccggacatac ccatcggcaa gcccacctgc cctgccgtca gcccttcagc gatttcaacc
     8881 ggcagagtga ccgtgttgcc atcgtaacta aaggagacgc gtgtacctgc gtttacaccc
     8941 aacttcgcgg catccgctgg gttgagtttg atgtacggct gcggcatacg acactggaag
     9001 accggagcac gctgtgacaa ttcatcgctg ccaaacaggt ggtagtacgg cgcgatacgc
     9061 catttcccgt cctgcggctg gaagcgtgcc ggtacgctgg tgaagtaatc cagaccattt
     9121 tcgctggttt caaacagacg cacacccgga tcgccaaagc gcagtttgcc gcccacttcg
     9181 tcctggaatt tgttccacgc ctgcggggag ttccagcccg gagcccaggc aaacggcact
     9241 tgcgaacggt gcgcggtcgg ctggttgtta ccttccatcg agaaggtgaa catggtgtca
     9301 atatcctgcg gctgacgcgg ctcatgaacg ctgatattgg cgcgcatggc ggtacgaccg
     9361 ctgtaacggt gcggttcacg ggccagtttc tgcccacgaa tacggaatgt cgcatctggc
     9421 gcagcatctt tgatacccgc cagttccggg attttcgcca caacagcgtc aattacatgg
     9481 tcgagctgcg tccagtccac ttcacggctc agcaaggtgc tgtgcagcga gtgcagccag
     9541 cgccaacttt ccagcatgac agttttgctg tcgtaataag ccggatcgta aacctggaag
     9601 aaacgttggg cgcgaccttc attattaatt accgtaccgt cgctttcagc aaagctggca
     9661 gcggaaagca ccagatgggc gttttccatg atcgctgtgc gttgatggtc aaccaccatc
     9721 accagcggcg ctttagccag cgcagcattc acgcgggtag cagaagcgtg acgatgcaga
     9781 tcgttttcca gcaccaccac cgcgtcggcg cgtccggttt ccagttcggt taacgcttct
     9841 tcaagcgatc caccgcccat aatgcccaac cccatgctgt tgacggaacg ggcaatcatg
     9901 gtgataccga cgtcagcgcc gcgacctttc agggctttcg cgacgttagc cgcagcctga
     9961 atcacctcta agctaccggc gttcgtcccg gagataatca acggtttctt cgcacccgcc
    10021 agtgcctgca cgatgacgtc gattttgctt tgcagctcag gttcaatacc gtcaaccgct
    10081 ggcgcagagt tatccagcgc atgggcgatg gcaaaaccta aacgcgcctg atcttcaacc
    10141 ggtgcgcggt aagtccacgc cgcgatatca tccagacggg tgtcatcaac gttggtaaca
    10201 aacagcggat gcttagcacg ttgaccgata ttgaggattg ccgcaatctg ccagtcagcc
    10261 actttctgtg ctgccgccat ttcgcgcgct ttacctttca ccgcctggcg cactgccagc
    10321 gcgacgcgcg cgccggtctg ggtaacgtct tcgcccagca ccagtaccgc atcgtaagat
    10381 tcgatttcgc gcagagccgg agtataaatg ccgccttcac gcagcacttt cagcgccagt
    10441 tgcagacgtt cctgctcacc gtgagcgata ccggtgtaga agttttcttc gcccaccagt
    10501 tcacgcagcg caaagttgct ttccacgctg gcacgcggag aaccaatacc gatcactttc
    10561 ttcgactgac gcagaatatc tgccgcgccc tgcattgcct gttcggcgtt gagggtaatg
    10621 aaatcatcgc cacgacgctg tactggctga cgcggacgat ctttcaggtt gacgtaaccg
    10681 taaccgaaac gaccacggtc gcagaggaag tagtggttta ccgtaccgtt gtaacggttt
    10741 tcgatacgac gcagttcgcc ataacgctcg ccggggctga tgttacagcc gatggaacat
    10801 tgctggcaga tgctcggcgc aaactgcata tcccatttac ggttgtaacg ctcggagtgc
    10861 gttttgtcgg taaatacgcc ggtcgggcaa atttcgacca ggttaccgga aaattcgctt
    10921 tccagcgtgc cgtcttccgg gcgaccgaag tagacgttgt catgcgcgcc ataaacgccc
    10981 agatctgtac cgtcagcgta atctttgtag taacgcacgc agcggtagca ggcgatgcag
    11041 cggttcattt cgtgagagat gaacggcccc aaatcctgat tacggtgggt acgtttggtg
    11101 aaacggtagc gacggaagct gtgtccggtc atcacggtca tatcctgaag atggcagtta
    11161 ccgccttctt cacataccgg acagtcgtgc gggtggttgg tcattaacca ctcgaccaca
    11221 ctttcacgga actgtttcgc ttcttcgtcg tcaatggaaa taaaggtgcc atcggaagcc
    11281 ggtgtcatac aggacatcac caggcgacca cgcgtgtctt ccgcgttttg gtattgcttc
    11341 accgcacact ggcggcaagc accgacgctt cccagcgccg gatgccagca aaagtaagga
    11401 atatcaaggc ccagagacag acaagcttcc agcaggttgt ccgctccgtt gacctcgtat
    11461 tctttgccgt ctacatgaat tgtagccatt agcatgcttc cagttttctc agtcagagac
    11521 tgagcgttaa tcgaaattcg gttaccagcg ctctttcagc aggttcggct gaatcccatt
    11581 aatcaaatgg gtattgctga acggctgttt gatccccgcc tcaaattctt cgcggaaata
    11641 tttgatggcg ctctgtaacg gctccacagc accaggtgcg tgggcacaga aagttttacc
    11701 cgggcctaag aatcgacaca gttgctcaag tgtttcgata tcgcccggct gaccttcgcc
    11761 acgctccagc gcacgcagaa ttttcacgct ccacggcaga ccgtcgcggc acggcgtaca
    11821 ccagccgcag gactcacggg cgaaaaactc ttccaggtta cgcaccagcg acaccatgtt
    11881 gatttcatgg tcaaccgcca tcgccagcgc cgtacccaga cggctgcccg ctttaccgat
    11941 actttcgaat tccatcggca ggtcaaggtg cgcttcggtc aggaagtcgg tgcccgcgcc
    12001 gcctggctgc caggctttga atttcagacc gtcacgcata ccaccggcgt aatcttcgag
    12061 gatctcgcgt gcggtggtgc cgaacggcag ttcccacagt cccggatttt tcacccgacc
    12121 ggagaagccc atcagcttgg tgccagcatc tttacttttc gagatgttct gataccactc
    12181 cacgccgtta gcgaggatcg ccggaacgtt acacagggtt tcgacgttgt tgacacaggt
    12241 cggtttgccc catacgccgg aggttgccgg gaaggggggt ttcgagcgtg ggttagcacg
    12301 acgcccttcc agggagttga ttaacgctgt ttcttccccg cagatgtagc gccctgcccc
    12361 ggtatggacg aacagttcga agtcgaaacc tgttcccata atgtttttgc caagcaatcc
    12421 cgcttcagtc gcttcggcaa tggcgcggcg cagatttact gccgcttcaa tatattcgcc
    12481 acgcaggaag atgtagccac ggtaagcttt cagcgcaaac gcggagatga gcataccttc
    12541 caccagcagg tgcggcagtt gctccatcaa caggcggtct ttataggtgc ccggctccat
    12601 ttcatcggcg ttacacagca ggtaacggat gttcatggat tcgtctttcg gcatcaggct
    12661 ccacttcaag ccagtcgaaa agcccgcgcc gccgcgccct ttcagaccag cgtcttttac
    12721 ctgattaacg atttcgtccg gagacagccc ggtcagcgcc ttacgcgccc cttcgtaacc
    12781 gtttttgctg cggtattcgt ccagccacac tggctgtttg tcatcgcgca gacgccaggt
    12841 cagcggatgc gtttcgggag tacggataat gtttttcatt tataccgctc cagcagttca
    12901 gggatcgctt ccggggtcag attcgcgtga gtgtcctcat cgatcatcat gtttggccct
    12961 ttatcacagt tccccaggca gcaagttggc agcagcgtaa agcggccatc aaatgtcgtt
    13021 tgccctggtt tgatgttcag ctttttctcg agcgccgcct gaataccctg atacccattg
    13081 atatgacaga ccacgctgtc gcaataacgg atcacatggc ggccaaccgg ctggcggaag
    13141 atctggctgt agaacgttgc cacgccttcg acgtcgcttg ccggaatacc cagcacatcg
    13201 gcgatcgcgt ggatcgcacc atccggcacc cagccacgct gcttctgaac gattttcagc
    13261 gcttcaatgg acgccgcacg cgggtcttcg tagtggtgca tctcgtgctc gatcgcttca
    13321 cgctctgccg cactcagctc aaaagcctcg gtttgtggtt gttgattctc gtgcataatt
    13381 agcggtccac atctgacata acaaaatcga tactgcccag ataaacaatc aggtcagaca
    13441 ccaggctgcc gcggatcgcc gccggaattt gctgcaaatg cgcataactc ggcgtgcgga
    13501 tacgagtgcg ataactcatg gtgctaccgt cgctggtcag gtagtaactg ttgatccctt
    13561 tggtcgcctc aatcatctgg aaggattcat tggcaggcat caccggcccc cacgacactt
    13621 gcaggaagtg ggtgatcagg gtttcgatat gttgcagcgt gcgctctttc ggcggcggcg
    13681 tggtcagcgg gtgatccgct ttgaacgggc cttccggcat gttgttgagg cactgctcaa
    13741 gaatgcgcag actctggcgc agctcttcca ctttaagcat tacgcgggtg tagcagtcag
    13801 aaacaccacc accaaccggg atttcaaagt cgaagttttc atagccagaa taaggacgcg
    13861 ccttacgcac gtcgaagtcg atcccggtag cacgcaggcc cgcgccagtg gtgccccact
    13921 ccagcgcctc tttcgcgcca taggcggcaa cgccctggga acgacctttc agaatggtat
    13981 tttgcagcgc cgctttctcg taagacgcca gacgtttcgg catccagtcg aggaactcac
    14041 gcagcaggcg atcccagccg cgcggcaggt cgtgcgctac gccgccaata cggaaccacg
    14101 ccgggtgcat acggaaaccc gtgatcgctt ccaccagatc gtaaattttc tgacgatcgg
    14161 taaaggcgaa gaacacgggc gtcattgcgc cgacgtcctg aataaaggtc gagatataca
    14221 gcaggtggct gttgatgcgg aacagttcgg agagcattac gcgaatgacg ttaacgcgag
    14281 ccggcacggt gatcccggcc agtttctcta ccgccagcac gtaaggcatt tcgttaacgc
    14341 agccgccgag gtattcgata cggtcggtat acggaatgta gctgtgccag gactggcgtt
    14401 cgcccatttt ctccgcacca cggtggtggt agccgatgtc tggtacgcag tcgacaatct
    14461 cttcgccgtc gagctgcaaa acgatacgga aagcaccgtg cgccgacggg tggttcggac
    14521 cgaggttgag gaacatgaag tcctcgtttt cggtgccgcg cttcatcccc cactcttccg
    14581 gtttgaaggt cagggcttcc atctccagat cctgtttggc tttggtcagc tcaaacggcg
    14641 agaattcggt agcgcgcgcc gggtaatctt tacgcagcgg gtgacctttc caggtttgcg
    14701 gcatcatgat gcggcgcagg ttcgggtgac cgtcgaatgt aatgccaaac agatcccagg
    14761 tttcacgctc ataccagtta gcgttcggga acagtttggt gaaggtcggt acgtgcaggt
    14821 cgttttctgc cagcgccacc ttcagcatga tgtcgcggtt acgatcgata gaaatcagat
    14881 ggtagaaaac ggaaaaatcc gcggcaggta acccttcgcg gtgcgtgcgc agacgttcgt
    14941 ccatgccgtg taagtcaaac agcatgacgt aaggtttcgg cagtttcttt aagaaatcgc
    15001 caacttccag taattgttca cgcttgatcc acacaacggg aaccccggtg cgagtcgcct
    15061 gaacagtaaa ggcatccggc ccaaaacggt tgcgcagttc gccaattacc ggatcatcaa
    15121 gatgatcgcg ggtctgccag gcgggttctt gcgcggttaa gtcggtcata ttgttcacca
    15181 ttgcaaatgg tccgtggtga ctgtcggcgc agcaagcttc gcgaaataga tttaggaata
    15241 agcgaagaaa aatccctttg ccgacaggcg caaattaaat ctcgtcaggt gtacgcaggt
    15301 tggttacggc aatgcgttca ccgcgcttgc gttcgcgctc tgattgcata ttggcgcgat
    15361 aaacgccctg atcgccaacc acccaggaga gcggacgacg ttctttgccg atagattcct
    15421 gcaacagcat cagtgcctgc atgtacgctt caggacgcgg cgggcagccc gggatataca
    15481 catcaaccgg gatgaattta tcgacgccct gcacaacgga ataaatatcg tacataccac
    15541 cagagttggc acaggcaccc attgagataa cccattttgg ttccagcatc tggtcataca
    15601 gacgctgaat aaccggtgcc attttggtaa agcaggttcc tgcaaccacc atcaggtcag
    15661 cctgacgcgg cgaagcacgc aatacttctg cgccaaaacg cgccacgtca tgcaccgcgg
    15721 taaacgaagt caccatctca acgtaacagc aggaaagacc gaagttatac ggccaaattg
    15781 agtttttacg accccagtta accatgtcat tgagcttgcc cataaacacg tttttgttaa
    15841 cttcttgctc cagagggtcg gttacgatct cctgcttttg cagggggtaa cggtcgttct
    15901 caccgttggg atctatgcgg gtgagcgtat aatccatctt aatgcctcgc ggttagcgtt
    15961 gacgattagc gatactgttc gtttccgggt tcatacgctc gcggcgtgaa cgcgcgggcg
    16021 tccagtccag cgcgccaata cgcaccagat aaaccagacc tgccagtaac acaaaaataa
    16081 aaattgcagc ttccacaaag cctacccagc cgctttcgcg gatagaggtt gaccatgcga
    16141 acagatacag cgcttcaacg tcgaagataa cgaagaacat ggccaccaga taaaacttgg
    16201 cggacaggcg taagcgggcg gagccgaccg agtcgatacc ggattcaaac ggcacgtttt
    16261 tcgacctcgc gcgtgcgcga ccgcctaaaa accaaccgcc taccagcatc aggcaacata
    16321 ggccaatggc aacgataaga aagatagcga atgcccagtg atgagcgatg acttcagtgg
    16381 atgttgacat actcattgct tactcatcaa aagtagcgcc agattcactg ctcttcacgg
    16441 cagatggacg ccacatcgat tcatggggag gaataaaaaa aaccttacaa tcactgtaga
    16501 aattctttta tacagctaat tgatgtggtc ttttactcct ttctataacc ttttgtcaac
    16561 tttaacaaaa gtttcttcac attagtttac ataatatcaa caccattagc atttaatgcc
    16621 ctttcacccc agatccttga cgactccagg ataattagat gttgttgaat cgtgtccgtt
    16681 gtgaagcaat ggaaaaaata cgggtctatt ttgacaggaa tttgtgtcga ttcctccccc
    16741 caaaagagag tattttcttg atctgtgaca cgcttttgtc attccataac aaaaacgcag
    16801 caacaaattt acgtattttt taacatcatt gtagcaggtg atttttttca ggcgattatt
    16861 tgtgcgtacg gggcgtgaat ctctggtgat tgaaaaatga acagttttgt acgttctgca
    16921 cgatgtgaag aaggttcttt actaaaaaaa agccgctggg gtttaaaaca cccccagcgg
    16981 ctcgtttttt acactattgt ctcaggaatt atctatcgtc cgtcgattac tcgatatccc
    17041 tttcaatcaa caatgaatca tccccttccg gagcagatat tgggctgtat tgccacggat
    17101 tatggtagct ctccatcgcc tgatagatca cctgggccag ctcattatta ctggacgggt
    17161 catagcacag caaatattcg gtatcaggta gcggcggtaa gccatctact ccactcagca
    17221 cacgcaagtc cggactcatc atctcgaccg gccttgccgt cacgccaaga ccggctttca
    17281 ctgccgcacg aacggccgga agcgtcgagg cgacataagc cagtcgccag ggaatatctg
    17341 ctttattaag cgtcgccagc accatatcgc gaaacggact tggatcatcc agcaatacaa
    17401 gagggatcgg ctcacctttt tgcaatacgt attccgctgc gcagtaccag tgtgttggcg
    17461 aggtacgcag gttcaatgcc ttaaacgctg aaggacgatg ggtggttacc atcaaatcca
    17521 cttcctggga ttccagcatt tctgccatat aggcattacg tttaacgcgg acatccagcg
    17581 ctaatttcgg ataaaccgaa ctcacgcgat ttaacaggaa aggtaagatc gtatcggcag
    17641 attcatctga agcaccgata gttaacacgc cctgaagatt actgaacatt aatgatgagc
    17701 aggcctcatc attaaaacgc aggattttcc tggcgtaacc aagaagttga atgccatgtt
    17761 cagttaacag tttgttgcga ccgtgacgag cgaacagttc tttcccaacg agttgttcca
    17821 gacgctgcat ttgctgactt acggcggact gagtacgaca cacagcggca gctgcggcag
    17881 caaaagtgtt cagatcggca acagcaacaa atgttctcag cagatcgagg tcgaggttaa
    17941 ttatcggacg atttgcactt atcatatatt atcacttact ggcggctcat actgagctgg
    18001 ttaatgctgt gcacacacaa acaagcaatt ccatttgtaa tgtgcctccc tggcagtttc
    18061 atccggaacc cggacgaaag taaaaatgca tatgagttgc actaaaaaag cgactcacat
    18121 tgttccgtta taatgcctga agtagatcac agaatatatc ttcagggatc gcatatctat
    18181 taagttactc actcttttct atttatgaca tgcgcgtgtt tgtataaatg taaatgtgag
    18241 tccttgttcc actctcgtgc agcatcgctg gtcatacgcg aacacgtaca aacagcaatg
    18301 gtgaggcggc atcaagagca gggatccgtc atttatgcga gcattttacc ccaaaaacct
    18361 tttatttata agggtcattg cgaattatct gatgcaaagt tatgttatgt taggcaaagt
    18421 aatcttctat tattaataag cacatcaaaa cctttttgaa tattaaataa taattaatta
    18481 gcatcatcct cattcattaa ttccgcttaa caatagtttc acaccttgcc cccattgcga
    18541 cacctcggaa atcatcacgc tgttaaactc tccgtaatgc ggaaatatca tcatcgcgtt
    18601 tcattagtga attcttaaat gaggcatttt cacacaatta tcttacagat aaaaaaacca
    18661 gacttacaat taagaatcag aacaattcac tatataacat ttcatgtaaa gcatatgcac
    18721 ctcattattt tgtcattatt aagttattaa cagcacaatc gagccttccc ctctggcaaa
    18781 atcttattct gcagaccttc aaaacaccgt cctgggggag tacattgttc taagctgact
    18841 tccacggcag ggagtggcga taacagcaaa aaaggtcaag attcatgtcc cccattgaaa
    18901 aatccagcaa attagagaat gtctgttatg acatccgtgg tccggtgctg aaagaagcaa
    18961 aacgcctgga agaagaaggt aacaaggtac tgaaactgaa catcggcaac ccagccccgt
    19021 tcggttttga cgcgccagat gaaatcctcg ttgacgtgat acgcaacctg cctaccgctc
    19081 aagggtattg cgattccaaa ggtctttact ccgcgcgtaa agccatcatg cagcactatc
    19141 aggctcgtgg catgcgtgat gttaccgtgg aagatattta catcggcaat ggtgtatcgg
    19201 agcttatcgt tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg ttggttcctg
    19261 caccagatta cccactatgg accgcggcgg tttcgctttc cagcggtaaa gcggtgcatt
    19321 atctttgcga tgaatcctct gactggttcc cggacctcga tgatattcgc gctaaaatta
    19381 cgcctcgtac gcgtgggatc gttattatca acccaaataa cccaaccggc gcggtatatt
    19441 ccaaagagct tttaatggag attgtggaga ttgcacgtca gcataatctc attatcttcg
    19501 ccgatgaaat ttatgacaaa attctctacg acgacgctga gcatcactca attgcgccgc
    19561 tggcacctga cctgctgacc attaccttta atggactgtc gaaaacgtac cgcgttgcag
    19621 gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa ggctacatcg
    19681 aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca
    19741 ttcagaccgc gctgggtggt tatcagagca tcagtgaatt tattacccct ggcggtcgtc
    19801 tttatgagca gcgtaaccgc gcgtgggaat tgatcaacga tattccgggc gtttcctgcg
    19861 tgaaaccttg tggtgcgctg tacatgttcc cgaaaatcga cgccaaacgc tttaacattc
    19921 acgacgatca gaaaatggtg ctggatttcc tgttgcagga aaaagttctg ttggtgcaag
    19981 ggacggcatt caactggccg tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg
    20041 atgatatcga gctgtcgttg agcaagttcg cgcgtttcct ttctggttat catcagctgt
    20101 aatcttaatt tcactgccgg agattgcatc cggcagtgtt atcccgccac aatgacctga
    20161 tgatgtcatc atacgtaagg tcactatgaa acagagccat ttttttgccc atctctcccg
    20221 cctgaaactc attaaccgct ggccgctcat gcgcaacgtg cggacggaaa atgtgtccga
    20281 acacagtttg caggtagcga tggtcgccca tgcgctggca gctatcaaaa atcggaaatt
    20341 tggcggtaat gtcaacgccg aacgtatcgc tttactggcg atgtaccacg atgcctcaga
    20401 agtgctcacc ggcgatctcc ctactccggt gaaatacttc aattcgcaaa tcgctcagga
    20461 gtacaaggct attgaaaaaa tcgctcagca aaaactggtc gatatggttc cggaagagct
    20521 gcgggatatc tttgcgccgt taattgacga gcatgcatat agcgatgaag aaaaatcgct
    20581 ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac tcgcggcggg
    20641 aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag cgcgccgcag
    20701 ccaggagatg gactacttca tggaagtatt tgttcccagc ttccatcttt cgctcgatga
    20761 gattagccag gattcaccgc tgtaagcagc tggagtctgc gtcgcatcag gcaataagcg
    20821 ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat caccacacaa
    20881 accgccatca cgataatggt gaacggtacc cccagcttca caaagtcact gaagctgtaa
    20941 tttcccggac ctaaaaccaa tgtgttaaca ggtgaagaaa ccggtgtcat aaaggcggcg
    21001 gatgctgcca tcgccacgac catcgcgaat ggataaggcg acacccccat cgttttggca
    21061 gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagagat aaatagccca
    21121 ataaccgccg acaagacaaa caaacaaccc agcatcatat gtggcccgta accaccgcca
    21181 atgtccatca gccctttcac cgccagcgcg acacctcccg ttttctgtaa tgccacagca
    21241 aacggcatca tcccaacgat caaaataatg ctcggccagt gaatggattt ataggcgctt
    21301 tcagcatcta tacagcggaa tttccccatc agcaggcagg cgatgatagc ggcaacagga
    21361 ttaggaattt catctgtcag cattaacgcc accattagca ccagacagaa aatggcatgg
    21421 ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt cagcgctacg
    21481 aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac aaccaggatg
    21541 atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac gccattgcgc
    21601 tttagcccca ccacattcag tccgtagcgg gtacgaaaac caatttcgcg caccgattta
    21661 ccaatcagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc aagggcctgg
    21721 tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact acaaaattgc
    21781 cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc gcgaaattca
    21841 gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc aacaccgatg
    21901 acgttagcgc cataacgctc acgtaatttg agatcatcca gccgttgacc aatcattggc
    21961 gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc acggataaga
    22021 tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc ccctttcagc
    22081 atgaaacgca tcactaacat atacaaaata cccagcacca gcacgaccag gccaataggt
    22141 gttacgctaa agaaactgaa gccgtgatag ccttcacgca gcaattcact gttgactacc
    22201 aggttcggcg gcgtcgccac cagcgtcatc atgccgctaa tcagcccggc aaaacttaac
    22261 ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg caacgcttaa caccacggga
    22321 ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc gacggtcagc
    22381 atcaacaaaa ccaacatttt gatttcactg ttgcccgcaa ctttgaccag ccatgttccc
    22441 attacggtgg caacaccggt acggaccaaa ccatcgccaa taataaacaa ggcggcaatc
    22501 aggacaacgt taggatcaga aaagccagaa aatacttctg ggactgtcag cgttccgctt
    22561 aatgcaaacg cgacaataac aaacaaagcg accgcatcca tacgcactct gcccgtcgca
    22621 aacaagacga tggcaacggc cagtaatgaa agaacccaaa tcaattcacc gttcacaact
    22681 tatccttgtt aattgagggg gatgacttga ttctgccata aaaaagcccc gacgagacgg
    22741 ggctaaatca tgatcaggtg tttcactgaa taataacatc gccatttggc tgtttggtca
    22801 cagtaatttg ctccagactg tggaggacca aatcgacctc attcaggcgc ggggtatctg
    22861 ccggagcgtt aaccgcaatg acatggcaac ccgccgccag gccagaaagc acgccagcgg
    22921 gagcatcttc caccaccaca cactcctgcg gcgcaagccc cagcagctgc gcgcctaaca
    22981 gatacgcatc aggttctggt tttccgcgct tcactcgctc agcggttaca aacacctctg
    23041 gtgcgggaag ccccgctatt ttatggcgcg ctcgcgctac cggcatggag ccagaagtca
    23101 caatggccca cggaatacct gctttattca aatgattgag taaggcgatt gcccccggaa
    23161 gcgcggtaat accttcggtt tccgtggcct cgatttgctc caaacgcgta aactcggcgg
    23221 caatatcagc ctcggatttg cccgccataa aatggcgcag agaggtgatc gcctgtttac
    23281 cgtgaatgaa agccagcacc tcttccggcg ctaacccatg acgtctggcc cagttgctcc
    23341 acgcccgttc taccgcaggc agggaatcca ccagcgttcc atcaagatca aacagaaaac
    23401 ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt gattaatttc gttggcgctc
    23461 aaatggtact gacgcgggca ggcatgccac acattaagca tgcgctgata tttttcccac
    23521 attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc
    23581 acattaacca taaagcggac ataaccgagg taacgtgctt cagtggcggc gtcaaagccg
    23641 aggaaggtga cacgacgttc atcgatggat tgctggtcct gcaaattaga ccaggaaaca
    23701 tgcaacgcat gatacatctc cataatgtcg atgatagtgc ggcaggtttc ttctttcagc
    23761 tcgccaaact cgcgatccag ttcacgcatc tgtaatccat aaccccgctc aatgattgtt
    23821 tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca tagtcatcat cttgtactgg
    23881 ttagacaaaa taagacgttg cgcgttggtc atttccattg ttgactcctg tatcactcta
    23941 ctacggtgaa aaaaaagaag gctgagtatg ccttctttta tatgcgtaat caggggtcaa
    24001 ttacaaatca tcaaggaaag ttttatccag ttgtttgaag gcacgcttaa gcgtgtcagc
    24061 taatgcctgg taatcaggct tgccttcaac gggtgccaac acctgtccag actcctgcaa
    24121 tttaccgcga acttcataaa accagttgag gattgcaggg ggtaatggcg tgacagaacg
    24181 cttgcccagc caccacaatc cctgcatggg taaacttaag gcgaacaggg cagtggcaac
    24241 tgccggccca agctgaccgc ccagggcaat ctgccagcag agagtaaata cggcgatcgg
    24301 cggcataaaa cggatcgcat aacgcgtcat cttgataacg cgattttcga caaagaccgg
    24361 ggcaaggcgt ttttccagcg gccacgtctt tgagtaatgc tgtccccggc gaaacaagct
    24421 aaaaaaatta acagaacgat tatccggcgt tgacatgctt cacctcaact tcacatataa
    24481 agattcaaaa atttgtgcaa attcacaact cagcgggaca acgttcaaaa cattttgtct
    24541 tccataccca ctatcaggta tcctttagca gcctgaaggc ctaagtagta catattcatt
    24601 gagtcgtcaa attcatatac attatgccat tggctgaaaa ttacgcaaaa tggcatagac
    24661 tcaagatatt tcttccatca tgcaaaaaaa atttgcagtg catgatgtta atcataaatg
    24721 tcggtgtcat catgcgctac gctctatggc tccctgacgt ttttttagcc acgtatcaat
    24781 tataggtact tccatgtcga gtaagttagt actggttctg aactgcggta gttcttcact
    24841 gaaatttgcc atcatcgatg cagtaaatgg tgaagagtac ctttctggtt tagccgaatg
    24901 tttccacctg cccgaagcac gtatcaaatg gaaaatggac ggcaataaac aggaagcggc
    24961 tttaggtgca ggcgccgctc acagcgaagc gctcaacttt atcgttaata ctattctggc
    25021 acaaaaacca gaactgtctg cgcagctgac tgctatcggt caccgtatcg tacacggcgg
    25081 cgaaaagtat accagctccg tagtgatcga tgagtctgtt attcagggta tcaaagatgc
    25141 agcttctttt gcaccgctgc acaacccggc tcacctgatc ggtatcgaag aagctctgaa
    25201 atctttccca cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt tccaccagac
    25261 tatgccggaa gagtcttacc tctacgccct gccgtacaac ctgtacaaag agcacggcat
    25321 ccgtcgttac ggcgcgcacg gcaccagcca cttctatgta acccaggaag cggcaaaaat
    25381 gctgaacaaa ccggtagaag aactgaacat catcacctgc cacctgggca acggtggttc
    25441 cgtttctgct atccgcaacg gtaaatgcgt tgacacctct atgggcctga ccccgctgga
    25501 aggtctggtc atgggtaccc gttctggtga tatcgatccg gcgatcatct tccacctgca
    25561 cgacaccctg ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag agtctggcct
    25621 gctgggtctg accgaagtga ccagcgactg ccgctatgtt gaagacaact acgcgacgaa
    25681 agaagacgcg aagcgcgcaa tggacgttta ctgccaccgc ctggcgaaat acatcggtgc
    25741 ctacactgcg ctgatggatg gtcgtctgga cgctgttgta ttcaccggtg gtatcggtga
    25801 aaatgccgcg atggtccgtg aactgtctct gggcaaactg ggcgtgctgg gctttgaagt
    25861 tgatcatgaa cgcaatctgg ctgcacgttt cggcaaatct ggtttcatca acaaagaagg
    25921 tacccgtcct gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag
    25981 ccgcctgact gcctgatttc acaccgccag ctcagctggc ggtgctgttt tgtaacccgc
    26041 caaatcggcg gtaacgaaag aggataaacc gtgtcccgta ttattatgct gatccctacc
    26101 ggaaccagcg tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa
    26161 ggcgttcgtc tgagcgtttt caaacctatc gctcagccgc gtaccggtgg cgatgcgccc
    26221 gatcagacta cgactatcgt gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg
    26281 aaaatgagct acgttgaagg tctgctttcc agcaatcaga aagatgtgct gatggaagag
    26341 atcgtcgcaa actaccacgc taacaccaaa gacgctgaag tcgttctggt tgaaggtctg
    26401 gtcccgacac gtaagcacca atttgcccag tctctgaact acgaaatcgc taaaacgctg
    26461 aatgcggaaa tcgtcttcgt tatgtctcag ggcactgaca ccccggaaca gctgaaagag
    26521 cgtatcgaac tgacccgcaa cagcttcggc ggtgccaaaa acaccaacat caccggcgtt
    26581 atcgttaaca aactgaacgc accggttgat gaacagggtc gtactcgccc ggatctgtcc
    26641 gagattttcg acgactcttc caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa
    26701 gaatccagcc cgctgccggt tctcggcgct gtgccgtgga gctttgacct gatcgcgact
    26761 cgtgcgatcg atatggctcg ccacctgaat gcgaccatca tcaacgaagg cgacatcaat
    26821 actcgccgcg ttaaatccgt cactttctgc gcacgcagca ttccgcacat gctggagcac
    26881 ttccgtgccg gttctctgct ggtgacttcc gcagaccgtc ctgacgtgct ggttgccgct
    26941 tgcctggctg ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa
    27001 atggacgcgc gcatttctaa actgtgcgaa cgtgctttcg ctaccggcct gccggtattt
    27061 atggtgaaca ccaacacctg gcagacttct ctgagcctgc agagcttcaa cctggaagtt
    27121 ccagttgacg atcacgagcg tatcgagaaa gttcaggaat acgttgctaa ctacatcaac
    27181 gctgactgga tcgattctct gactgccact tctgagcgca gccgtcgtct gtctccgcca
    27241 gcgttccgtt atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg
    27301 gaaggtgacg aaccgcgtac cgttaaagca gccgctatct gtgctgaacg tggtatcgca
    27361 acttgcgtac tgctgggtaa tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta
    27421 gaactgggtg caggcattga aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt
    27481 cgtctggtcg agctgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg
    27541 gaagacaacg tggtgctcgg tacgctgatg ctggaacagg atgaagttga tggtctggtt
    27601 tccggtgctg ttcacactac cgcaaacacc atccgtccgc cgctgcagct gatcaaaact
    27661 gcaccgggca gctccctggt atcttccgtg ttcttcatgc tgctgccgga acaggtttac
    27721 gtttacggtg actgtgcgat caacccggat ccgaccgcag aacagctggc agaaatcgcg
    27781 attcagtccg ctgattccgc tgcagccttc ggtatcgaac cgcgcgttgc tatgctctcc
    27841 tactctaccg gtacttctgg tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt
    27901 ctggcgcagg aaaaacgtcc tgacctgatg atcgacggtc cgctgcagta cgacgctgcg
    27961 gtaatggctg acgttgcgaa atccaaagcg ccgaactctc cggttgcagg tcgcgctacc
    28021 gtgttcatct tcccggatct gaacaccggt aacaccacct acaaagcggt acagcgttct
    28081 gccgacctga tctccatcgg gccgatgctg cagggtatgc gcaagccggt taacgacctg
    28141 tcccgtggcg cactggttga cgatatcgtc tacaccatcg cgctgactgc gattcagtct
    28201 gcacagcagc agtaacctcg tcatcatccg cagctttgcg ctgcggatat ctgaaccgga
    28261 aatagtcact atttccggtt ttttattctc ttaatttgca ttaatccttt ctgattatct
    28321 tgcttaactg cgctgcatca atgaattgcg ccatctcact ttgcatactt accactttgt
    28381 tttgtgcaag ggaatatttg cgctatgtcc gcaatcactg aatccaaacc aacaagaaga
    28441 tgggcaatgc ccgatacgtt ggtgattatc ttttttgttg ctattttaac cagccttgcc
    28501 acctgggtag ttccggtggg gatgtttgac agtcaggaag tgcagtatca ggttgatggt
    28561 caaacaaaaa cacgcaaagt cgtagatcca cactcatttc gccttctgac taacgaagca
    28621 ggcgaacctg agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg
    28681 atgaacttcc cgtttgaagg gttaacctca ggatcgaaat acgggacagc cgttggcatc
    28741 atcatgttta tgctggtgat tggcggcgcg tttggcattg tgatgcgtac aggaaccatt
    28801 gataacggta tcctggcgct tattcgccat acccgtggga atgaaattct ctttattcct
    28861 gcgctgttta ttctgttttc acttggcggc gcggtatttg gtatggggga agaggccgtc
    28921 gcctttgcca ttatcatcgc accgctaatg gtccggctgg gctatgacag tattaccacc
    28981 gtcctggtga cctatattgc cacgcaaatc ggttttgcca gttcgtggat gaacccgttt
    29041 tgtgtggtcg ttgctcaggg gattgccggc gttccggtgc tttctggctc cgggttgcgc
    29101 atcgtggtgt gggttatcgc cactctgatt ggcctgatct ttaccatggt gtacgcctca
    29161 cgagtgaaaa agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa
    29221 aagcaggcgg atgttgaaca acgtccgttt acctttggtg actggctggt attgattgtc
    29281 ctgaccgccg taatggtctg ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca
    29341 gaaattgcca gccagttctt caccatgggt ctggtgattg gcatcatcgg cgttgttttc
    29401 cgccttaacg gtatgacggt taataccatg gcttcatcct tcaccgaagg ggcgcgaatg
    29461 atgatcgccc ctgccctgct ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat
    29521 ggtgaagcgg gtgatgccag cgtgttaaat actatcctca acagcattgc caatgccatt
    29581 agcggtctgg acaacgcggt cgcggcctgg tttatgttgc tcttccaggc ggtatttaat
    29641 ttcttcgtga cgtccggttc tggtcaggcg gcgttaacca tgccgttact ggcaccgctt
    29701 ggcgatctgg tcggtgttaa ccgtcaggtt accgtgctgg ctttccagtt tggtgatggc
    29761 ttcagtcaca tcatttaccc gacctcagct tcgttaatgg cgacgctcgg tgtttgccgg
    29821 gtggacttcc gtaactggct gaaggtgggc gcgaccctgc ttggactgct gtttattatg
    29881 tccagcgtcg tggtgatcgg cgctcagttg atgggctacc actaaaaatg ttaagagccg
    29941 caattgcggc tcttttttca ttctgccgtt tcagtctcta cggcttcatt tttggcattg
    30001 cgcttcatcc acaacgctag cgctttcagc gagtctggag tgaactcatc gcagcgtgcg
    30061 gtgatttctt ccggcgtcag ccagcaaact tcactgactt catcttcctg tagggcgaag
    30121 ggaccgtgag agacgcagct gaacaatgcg ccccagacac ggcaattttt atcttcgaaa
    30181 tagaactgcc cgtgctcggc aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg
    30241 cgacgcgcgg attccagcag ttgctcatcg gcctggacta caccgccagc ggtcgcatct
    30301 aacatgccgg gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat tttgcccatg
    30361 ccatcatgca cgacgatgta agttgcacga tgacgcagac actgtgcccg catttgctcc
    30421 cggctggctt gtgcaatgac ttcgttctct tcattgacaa tatccaccca ttcagtactt
    30481 gccaaacgac gctgttccat tatcaggaaa ccttcttttt ctggcgctct tacggcgcat
    30541 tttgagttgt gggtaaatta cggattaatc gcgacctgcg caatgatact ttgatcattg
    30601 agtgcgatta cactaagtac gtcattatcc agcatgccat aactcgccgg attaccgcct
    30661 ttcggaatac tcaccgagcc ggggttgaag tggaaaatct ccccccgctg ttctgctact
    30721 ggcagatggg tatgaccgta aaccagcaca tcgttctggt ttaaagcagg cagattttcc
    30781 gggccaaaaa gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc
    30841 cacggcgcgg ttatcgggaa atgcagcagc atttgatcca cttcgctgtc gcagttgccg
    30901 cgcacagcga taaccttatg tgccacttca ttaagccgtt cagcgacttt ggctggcgcg
    30961 taaccctccg gtaaagcatt acgcgggccg tgattcaaca cgtcgccaag gatcaccagc
    31021 cactgggcac cgctttgggc aaacaactcc agaacacgtt ccgtcgccgg taacgaccca
    31081 tgaatgtccg atgcaaacat cagtttcatc actcactcct cgtcgaaaaa acgtaagcta
    31141 tgatactgga ttctgtcgtt gctatcagcc agattgcttc gctgaaagtg caataaaacg
    31201 ctggacagac gctcgctgcc actggaatgt tgcataatcc accaggcatt ctggcacctc
    31261 atcgccatgt agtaccaggc ggttaatcat tagcgccaga tcagtatcag caatgcacca
    31321 ttcaccaaat aaattcggct gacccagtac taacaaatgt tctgccatcg cgaacagttt
    31381 ctctgcactg gcttttccct cggccgttag tggcgctttt ttcgcccccg caaagacaac
    31441 atctgtcgga cgctcttcgc ggatgggcat cagatcgctg cgcaaccagg cctgaatctg
    31501 tcgtgcacgc gcacgatttt ctaaatcaag cggataaata cgttcccagg tcggtggcgc
    31561 aaatcgatct tccagatact ccgcaatggc agaagattca ctcaactcaa aatcatcgat
    31621 ttgtaataac ggcacacggc gtgtctgacc gtaaccttgc cacgtcggtt gcaaatgttc
    31681 accgctgtcg aggtcgatgg tcttgatatg aaacaacagg cctttttcct gcaacgccac
    31741 ccaagcggat aacacataag gggagaaaaa atgggcatct gaccaaagcg tgatagcggg
    31801 tttactcatt atgtcctcgg caataaaggg ggctggctat caaactatag agcctttttg
    31861 tcactgtcac gtgacgaata ctcacgtaag tcgccgcgct ttctataatt gtggtgttca
    31921 gatgattaga caaccggagc tccaatgatc gatctctatt tcgccccgac acccaatggc
    31981 cacaaaatta cgctgtttct cgaagaagca gggctggatt atcgcttgat aaaggtagac
    32041 ctggggaaag gcggtcagtt tcgcccggaa tttttgctca tttcgcctaa caacaaaatt
    32101 ccggcaattg ttgatcattc tccagccgat ggcggcgaac cgctaagcct ctttgaatct
    32161 ggtgccattt tgttgtatct ggctgagaaa acggggctct ttttgagtca tgaaacgcgt
    32221 gaacgcgccg ccacattaca gtggttattc tggcaggtag gcggactggg gccgatgctt
    32281 gggcaaaatc atcattttaa tcacgcagcc ccccaaacca ttccttacgc tattgaacgt
    32341 tatcaggttg aaactcagcg tctgtaccat gtactgaaca agcggctgga aaactcgccc
    32401 tggctgggag gcgagaacta cagcattgcg gatattgcct gctggccgtg ggttaatgcc
    32461 tggactcgcc agcgaataga cctcgctatg tatccggcag tcaagaactg gcatgagcgg
    32521 atccgttcgc gccctgccac cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt
    32581 tcggatagtt aacagaaaca ggttctcgtg tattatttca tcctaagtaa aacaacggag
    32641 aacctgcaat ggcacaacct gccgctatta ttcgtataaa gaaccttcgt ttacgtacat
    32701 ttatcggaat taaggaagaa gaaattaaca accgtcagga tattgttatc aatgtgacga
    32761 tccactaccc cgccgataaa gcgcgcacta gcgaagatat caacgatgcg ctgaattatc
    32821 gcaccgtaac gaaaaacatt attcagcatg tagagaataa ccgtttctct ttgctggaaa
    32881 aattaactca ggatgtgctc gatatcgcac gtgaacatca ctgggtgacg tatgctgaag
    32941 tggagatcga taaactgcac gcgctgcgct acgccgattc ggtatccatg accttaagct
    33001 ggcagcgtta accgccatat cgggaggctg catgaacata gtgatcaccg gagggacggg
    33061 attaattggt cgccatttga ttccacgttt gctggagctg ggccatcaaa tcacggtaat
    33121 gacgcgtaac ccgcagaaag ccagttccgt tctcggccct cgggtgacac tatggcaagg
    33181 gcttgccgat caaagcaacc tcaacggcat tgatgcggta atcaacctgg ccggagaacc
    33241 gattgctgat aaacgttgga ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa
    33301 tatcacgcaa aaactggtcg atttgattaa tgccagcgac acgccaccgt cggtactcat
    33361 ttccggctcg gcaacgggct attatggcga cttaggtgaa gtggtggtta ccgaagagga
    33421 accgccgcat aacaaattta cccataaact ctgcgcccgc tgggaagaaa ttgcctgtcg
    33481 ggcgcaaagt gacaaaacgc gagtgtgcct gctgcgtacc ggcgtagtgc tggcaccgga
    33541 tggcggtatt ctcggtaaaa tgctgccgcc gtttcgtctt ggcctgggcg ggccgattgg
    33601 ttccggtcgg cagtatctgg cctggattca tatcgatgat atggtcaacg gcattctctg
    33661 gctgctggat aacgagctgc gcgggccatt taatatggtt tcgccctacc cgatacgcaa
    33721 tgaacaattt gcccatgcgc tcggtcatgc gctgcatcgc ccggctattt tgcgcgtccc
    33781 cgcaaccgcc attcggctgt taatgggcga atcttcagta ctggtattag gtggacaacg
    33841 cgcgctgcct aaacggctgg aagaagcggg ttttgcgttt cgctggtacg atttagaaga
    33901 ggcgctggcg gatgtcgttc gctgatgtgg tttactgcaa acatccgcca gttaactccc
    33961 ggtgttacag ggttagtggc tttgcgcgat aagatcgtct ggtgaaagtc gggtcagcgt
    34021 caggatgact tcgtggtcaa atcctttttc cagcatctcc tgagcaatac gcagagcttc
    34081 ttcgtgtttg ccctgcattg cgccttcttc acgtaatctg tcagcaatgg tcatcagttt
    34141 ctccttttct tgtggtgcgc gttctgttat ctcaccaata aatgcacgga aacgctgggc
    34201 atctcccgtt tgtaatacat aattaaacag ggcttttagc tgtctgtcat tagtgttccc
    34261 tgtaactagc agcgaaacaa tttggtcgac taatcccaac agatcgcgct gacgaatatg
    34321 tttctgaatt aactccaaca gcgccatttt acgatgttgc ataatctcgt catccggcac
    34381 cacggtaata tccaccaacg gaaaagccga tgaatatatt ttacgggcaa tcgcaggctc
    34441 ggcaaattca tcaagccagc agagtgaata aggataagga cttctgcaac catgataaaa
    34501 cagcatcggg atcaccaatg gaagctcttt atagcccaca tcaagatggt tttgcattgc
    34561 cgcaatggaa taacgcatca tgcgaaaagc cattaattct tccggcttac tttggtgctc
    34621 tatcactaca taaagataac ccactccctc ctgcgttttc acagaccaca agaggtcgga
    34681 ataatattgc cgcaggtctt catcaataaa actgtttggt tccagtttaa gcgtcgttaa
    34741 atcacacagt ttgcgcagcg gcgcgggaag atgaatatca ataaaatccc gcgcggtgtc
    34801 tggatggcgt aaaaaagatt taaataccgc atcatgcggc gtggaagttg tcgatattgt
    34861 catggcgttc cgtcacccct caaatttaag atgacgcgac aataaccacg attaacaccc
    34921 aatgcagcag gcagttttta tctttggaca gcgaattcag aagtattttt agcggactgc
    34981 aacttttaca tttcagcgga agctgactcg caaattcccc ttgaaaatac tccggcagcg
    35041 ttagccaccg gagaggcaat tatttcagcg aacctttaag gaactgttgc agacgagggc
    35101 tttgcgggtt gccaaataac tgctccggcg cgccttcttc ctctattttc ccctgatgga
    35161 ggaaaatgac gtgagtagat acatggcgag caaagcccat ttcgtgggtc accaccacca
    35221 tcgtttttcc ctcttccgcc agctgctgca taatgcgcag cacttcaccc accagctctg
    35281 ggtcaagggc cgatgtcggt tcatcaaaca acagaacttc cggttccatt gccagcgccc
    35341 gcgctatgga aacacgctgt tgctgaccac cggacaggtg caccgggtat ttcccctgcg
    35401 cacgttcgtc tatcccgact tttgccagat acttcaccgc ccgctcgcgc gcttcctgct
    35461 tgctcaggcc caacacctga atcggcgctt ccatgacgtt ttccagcacc gtcatatggc
    35521 tccagagatt gaagtgctgg aataccatcg tcaggcgtgt gcgcagtaag cgcagttgat
    35581 ttttatcggc gactttgagt tgaccgtctt tgtcgcgcac cagattgatc gtctggccat
    35641 tgaccacgat cgacccttca ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac
    35701 ttttccccga tcccgacgat ccgatgatgc ttattacatc tccggcattc gcttgcagtg
    35761 acaccccttt cagcacttcg tgttcgccat agcgtttgtg caaatcgata acgtttaatt
    35821 tattctcgga catcgtattc tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt
    35881 ttttccgctc tgcgaaagag gctgatcagg acataagaga tgattaaata gagcaccgcg
    35941 gcaatgccga aggcggtaaa aggttgatac gtggcggcgt taatatcgcg ggctattttc
    36001 agcagatccg gcaccgtggc agtaaatgcc aacgcagtag agtgcagcat caggatcact
    36061 tcgttgctgt atgccggtaa cgcaatacgc agcgcagaag gcaaaataat gcagcgatac
    36121 attttaaaag tcgagaagcc ataggctctg gcggcttcaa tttccccatg cggcaccgaa
    36181 cggattgccc cagcaaaaat ctcggtagtg taagcgcagg tgttaagcgt cagcgccagc
    36241 acggtacagt tcaggccact gcggaaaaag gcgttaagaa actcggtccc cttaacaatc
    36301 tccagcgtat acatgccgga atagaacacc agcaactgaa catacagcgg cgtaccgcga
    36361 aaaatatagg taaataacca gattggaaac tggatgtatt tattactgga gacacgacca
    36421 atcgccagaa acagcgccag gactccgcct atcactaccg acaaaataag cagccacaga
    36481 gtgatcgcca caccagtaaa gcgataaccg tcggtccaca gcagcggttt ccagtattca
    36541 tgtaagattt cgatcacagg tcagccctct tcacacccac agagtagcgg cgctcaagga
    36601 acagcagcac accattggaa acggtggtga aaaccaggta aatcacgcca cagacgatag
    36661 cgaaatagaa cggttcccag gtacttttgc ctgccagctg cgtggctttg accacatctt
    36721 ccaggccgag taacgaaacc agtgcggtag atttgaggat cacctgccag ttgttgccaa
    36781 tgcctggcag ggcgtaacgc atcatcgccg gaaacatgat ccgccgaaac acttgcccac
    36841 gagtaaaacc gaacgccctc gccgcctcta tatgtccttt cggcactgcc ataaaagcac
    36901 cacggaacgt ttcggtaaaa taagcaccgt agataaaacc gagagtgata ataccagcga
    36961 ccatcggatc gatatcaatc tgcccgacgc ccatcgcctc cgtcaccgtg tttagcgcaa
    37021 tctgcaaacc gtagaaaatc agcagcatca acactaagtc cggcacgcca cgaatcagcg
    37081 tggtgtaccc ttcgaaaatc agccccgaaa gccgattttg cgagagctta ccgccagcgc
    37141 caattaaacc gatgattaca gcgagcacta cagagctgat agccagctcc agcgtgacga
    37201 gcgcaccctg taaaataaca cctgaaaacc catacaacat gctgcctgtc ctgtcgtgtg
    37261 tggtgaatta ccgcctcttg tctccctccg gttacccgaa gggagcgggc tttctttcac
    37321 cgatggcgat taaccaccat aaacatcaaa atcgaagtac tttttcgcta atttctcgta
    37381 agtaccgtca gcgcgcattt cggcaaaggc tttgttcagt gcctcgcgca gttcgttatc
    37441 ttctttacgc aggcccatgc cggtccctac gccaaacagt ttttcatctt taacagacgg
    37501 gccaccgaat ttgtaatctt taccgacagg ttgtttgagg aaaccttcgc tggcagcgac
    37561 ctcatcctgg aacgcggcat caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg
    37621 cccctgatac gagacgattt caatgccttt tggtgcccaa tgttcattac cgaacgtctc
    37681 ctgggtagtg ccctgcaata cgcctacccg tttgcctttc agcgactcga ctgtcggctg
    37741 aatgtcagaa tttttcgcca ccaccagacg agaatcggca gcgtacagtt tgtcggtgaa
    37801 ggctatttct tgctgacgtt tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt
    37861 cttcgctttt aaggacggga ttaacgcatc cagcggattt tcgacaaacg tacattgcgt
    37921 attgatgcgt ttgcataatt cctttgccag atcgatatcg aagccaacca gttcgccttg
    37981 tgaatttttt gattcaaatg gcgcataggt cgggtcggta ccgatgcgga tgttttgcgg
    38041 aatcgcagca aatgccgcag ttgcgctgga gaaggccaga accagagaga gcgatagcac
    38101 cagttttttc ataatgtcct caactgacag tctttttata gggaatattt acaggtttgt
    38161 tacacttatc gtgccataaa attgaccatc aaggcaaaat atcccgcccc gttagacgta
    38221 ttatttttca ttaaaaatgc ttaagtatgc atttcaatgc accacgatag tgcaacaaac
    38281 ctggcgtgca ccatgatgat cattccatca ggtacagctt ccctgcgacg tatcagtcac
    38341 cgtagacatt aaagtcaaaa tactttttcg ccatcttgtc gtaggtgccg tcctgacgca
    38401 gctcgccaag cgccttattg aaggcagccg tcagttcagc atcatcttta cgtagcccta
    38461 caccggtgcc atcaccgaag tattttttgt cttttactga tgagccagca aaggcgaaat
    38521 ctttaccagc aggttgcttg aggaatcctt cgctggcagc aacttcatcc tgcagtgcag
    38581 catccagacg tcctgcagcc agatcggaat agaccaaatc ctggttggca taggccacca
    38641 catccacacc tttgctacgc caggtctcgt tagcgtaagc ttcctgggtt gatccctgca
    38701 gcacaccaac atgtttacct ttcagtgagt ccagcgttgg ctgaatcggc gaacctttgg
    38761 cggcaatcag acgggaatct gcggcgtaca gcttgtcgga gaaggcaatc tcctgctgac
    38821 gtttatcggt aatggaaagc gacgaaataa tggcgtcgat ttttttcgct ttcagtgagg
    38881 ggatcagcgc gtcaaagtca ctggcaaccc aggtacattt cacctgcatc cgtttgcaca
    38941 tctcgttacc gagatcgata tcaaagccga caaagtcacc tttggcatct ttcgacgaga
    39001 acggtgcata ggtggtatcg gttccgatac gtaccgtctc cggcaatgcc gcatagctgg
    39061 aagccgctgc ggaaataccg actaacaaag acagagcgag aatcgacttc ttcatacata
    39121 accctcaagt gaaaatggct tttttatgtt gtgtattgtg ttgtgtgttt gcaggcttgt
    39181 tcatgcaggt cttatgccat cttgacgaca acagtaacat tcaacgttaa atatgttaat
    39241 aagacgttgc attattgtcc taaagttgaa gatagcaggt atagcggttg aatcgcagcg
    39301 tttcgattga atggcagaaa caaaatgtcg aggatttgat cgcggttgca aaattgccct
    39361 gaaacagggc aacagcggag ttatgcgccc tgccagcggg caaagagatc ttcaggaagg
    39421 gttatcgcaa actggtcaag aacacgatta accgtctgat ttatcacatc atcaagggat
    39481 tgcgggcgat gataaaacgc cggaacggga ggcataatca ccgcaccgat ttctgctgcc
    39541 tgcgtcatta aacgcagatg gcctaagtgc aatggtgttt cacgcacgca gagcaccagt
    39601 gggcgacgct ctttcagcac cacatctgcc gcacgggtca gtaagccatc agtatagcta
    39661 tggacaatgc cggacagggt tttgattgaa cagggtaaaa tcaccatccc cagtgtctgg
    39721 aaagaaccgg aagagatgct ggcggcaata tcgcgcgcat catgcgtgac atcagctaat
    39781 gcctgcactt cgcgcaggga aaaatccgtt tcgagggata aggtctggcg cgccgcctgg
    39841 ctcattacca gatgcgtttc gatatctgtg acatcgcgca gaacctgtaa taagcgcaca
    39901 ccataaatcg cgccgctggc accgctgatg cccacaataa gtcgtttcat aaatagttgc
    39961 cctgtcagac ttgcaggcag actttgcagg atttcggcgg gagttgcaag tcagggtgcc
    40021 agaccggcac cctgagcgaa ggcatcatcc ttcgttatgc atttcgagat tttccacttc
    40081 gttctgacgt tgcactgctt tggcgtcgtc attacgtaaa gtatcgagga aatcgaggta
    40141 gccctgatca acatctttgg tgacgtagac gccgttgaac accgagcatt caaactgctg
    40201 gatatccggg ttttcagcgc gaacggcttc gatcagatcg ttcagatcct ggaaaatcaa
    40261 cccgtcagca ccgatgatct ggcgaatttc atctacttcg cgaccgtgag cgatcagttc
    40321 cgtggcgctc ggcatatcaa taccataaac gttcgggaag cgaatttccg gtgccgcaga
    40381 agcgaggtac actttcttcg ctccggcttc gcgtgccatc tcgataatct gctcagaagt
    40441 ggtgccacgg acgatagagt cgtcgaccag caggacgttt ttatcgcgga actcggcgcg
    40501 gttggcgttc agtttacggc gcacggactt acgacgcagc tgctgacccg gcatgatgaa
    40561 ggtgcggcca acatagcggt ttttaacgaa gccctggcgg tacggtttgc ccagaatacg
    40621 agcaatttcc agcgcgatat cacacgaggt ttccgggatc gggatcacca cgtcgatatc
    40681 cagatcttcc cattcgcggg caattttctc gcccagcttc gtgcccatat tcacacgcgc
    40741 gctgtaaacg gaaattttgt cgatgaacga gtccgggcga gcaaagtata catactcaaa
    40801 caggcacgga ttgctgaccg gattgtcagc acattgacgg gtaaacaact gcccttcttc
    40861 agtgatgtaa atcgcttcgc caggcgcgac gtcacgcagg aaatcaaagc ccagtgtatc
    40921 gagcgcaacg ctttcggaag cgaccatata ttctgtgcgg ttctcgtcaa tatcacgttt
    40981 tcccagaacc agcggacgaa taccgtttgg atcgcggaaa gcaaccatac cgtggccgat
    41041 aatcatcgcc acacaggcat acgcgccgcg gattaagcgg tttgtagcag cgatggcggc
    41101 gaaaatgttg tcggcttcaa gcgggtagtg gcggaagttg tccagctcgc tggcgaagat
    41161 attaagcaga atttccgagt cggaagtggt gttgatgtgg cggcgttttt cttcaaacag
    41221 ttttttacgc aactcgtgag cgttggtcag attgccgttg tgggcaagcg taatgccata
    41281 cggagagtta acgtaaaacg gctgcgcttc agaggcgctg gagctgccag ccgtagggta
    41341 acgcacatga ccaatgccca tattgccctg caaacgctgc atatggcgag cttcaaatac
    41401 atcgctcacc agcccgttcg ctttacgcaa acggaagcag ttattggcat ctatggtgat
    41461 gatgccggcg gcatcctgac cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg
    41521 gttaaccggc ataacaccgg cgataccgac aataccgcac atacgtcttt ttcctcgtta
    41581 agccacatct cagagcactt acgctctggg caagaaactt gacgagcttt gcagataatc
    41641 aaaaaaccat ctgatgataa aactgaactg cggaatcagt tgtgatttac tccagtcttc
    41701 gcttttcgac accccggtaa aggagtcgag aaagaagaga atagcagcaa caatcaacac
    41761 accgcgcaat gcaccgaaac agacgcccag cacccgatcg gtgcctgaca accccgtttt
    41821 ctccaccagc aggcctatca cgaagttcac gatagcgcca acgatcaggg tggcgataaa
    41881 caggaccgcg atggcaatcc catttcgaac cagttcgtct tcaaagcccg taaaccagac
    41941 tgacaggtaa gtgtagtaat gactggcaac aaagaaagca caaccccatg tcaccagcga
    42001 taacgcttca cgaacaaagc cgcggatcag gctaaccaga gaggaaaaag caatcaccgc
    42061 gattatggcg taatcaatcc agaccatatg tgtcccacga ttttacgccc tgtcatcctg
    42121 ttcggggcgc attctaacag aaaaagaaaa cgtttgcgta gggatttcct tcccgcgcat
    42181 caataaaaat ggcgctgaaa aaatattcaa cgccatcgac tttttatgcc tttgcggcat
    42241 cggacaatgc gtgtcggatg cggcgtaaac gtcttatccg acctacggtt ctacccctgc
    42301 gtaggcctga taagacgcac cagcgtcgca tcaggcaacg gctgtcggat gcggcgtgaa
    42361 cgccttatcc gacctacggt tctacccctg cgtaggcctg ataagacgca ccagcgtcgc
    42421 atcaggcaac gcctgtcgga tgcggcgtga atgccttatc cgacctacgg ttctacccct
    42481 gcgtaggcct gataagacgc gccagcgtcg catcaggcaa cggctgtcgg atgcggcgta
    42541 aacgccttat ccgacctacg gttctacccc tgcgtaggcc tgataagacg cgccagcgtc
    42601 gcatcaggca agaccgtatt aattcggcgt atagcccatt accacgccac ttaagccaga
    42661 aagttgcttc aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac
    42721 cagaatacgg gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc
    42781 ggcaccgcgc agcttaccga caatctcatt cactttatcg gcatttttca gcgcgcccag
    42841 ttgcacaaca taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc
    42901 tggcttcggc gcaggtggtg cttcaacctt cggcttaggc ggctccaccg gtttcggctt
    42961 cggtgggacg accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc
    43021 cggatcgagc gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg
    43081 cggttgcgtc ggcaacgcct gggtggcggc tggcatcata tcaggctcat cgcgatcgcc
    43141 cgcttttggc accagcggga tagccgcaaa ctcatcctga taatgttttt tctgcccgtc
    43201 cagcagccct ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg
    43261 attctgaaac ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga
    43321 ccgtgtggaa cgaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg
    43381 catcccatgc ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt
    43441 gttctgccgt ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc
    43501 ttttcaacca ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca
    43561 gcacgcgccc gtttttcggt agcgttttca tacgcccggt gagatattcc gccgcatgtg
    43621 gattatgcgc gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg
    43681 gcaaaattgc gctggcaatc ccgtcgcgaa tggcattttc actgacctcc agcccgctgg
    43741 cacgcagtgc cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcaggt
    43801 tttccagcgt gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc
    43861 actcaacgcc ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag
    43921 aaggcatttc cggctcaccg acaattgccg gtttttcgct gcgaaagatg cctgctttct
    43981 cgcgaccaat actttcgcga tctggcccca gccagtcggt atgatccagc gcaatactgg
    44041 ttactaccgc gacatcggcg tcgacaatat tggttgcgtc cagacgaccg cccagcccta
    44101 cttccagaat caccacgtca agttgtgcct gtttgaacag ccacaacgcc gacagcgtac
    44161 cgtactcgaa ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg
    44221 cggtgtgggc cgattccggc aattcctgac cctgcacacg tacgcgctcg gtataacaca
    44281 ccagatgagg cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca
    44341 gcgtacggca ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg
    44401 gtttcagaac gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag
    44461 ttttactgtg caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag
    44521 gagtgcgttt gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca
    44581 gagccagtgg ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg
    44641 gtaccactac gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg
    44701 ccagaatgct cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc
    44761 ctttctcgat caggaattca ctgcgctgga atcctggcgg cagtttttcg cgaacggtct
    44821 gttcgataac acgcggaccg gcaaagccga ttaacgcttt cggttcagcg atgttgagat
    44881 cgcccagcat ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga
    44941 tgtacggcaa gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca
    45001 tcagcgacat cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg
    45061 ggcagttatc ttccagcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc
    45121 ccattgaacc gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca
    45181 gagtgccttt catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca
    45241 gacggtcttt atacttcttg gagtcacgaa acttcagcac atctttcggc tcaagctcgc
    45301 tacccagctc cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca
    45361 tacgcatgtg atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt
    45421 ataaaacctg accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc
    45481 gggtgggagt aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct
    45541 gaacctggtt cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac
    45601 agaccatgaa tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc
    45661 gaaccgcgga gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat
    45721 ttcgatgacg cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt
    45781 atcctgaacc atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca
    45841 caacagtgcg ccgatcacgt tataagcagc gaaatgacgg tacgacatgt ggcccattcc
    45901 cgcaacaaac ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt
    45961 gccgccatgt ttctcataaa actgatgggt tttatcgaga taactgcgac ggaaaatttt
    46021 cgaattggga ttactgaata acttttcacc gaacaagcgc ccgatagtgt aattgaccgc
    46081 gtcacccaca atcgcggcga tcagcatcag caccaccatc atatggacgt tgagatcgtt
    46141 ggtttccagt gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg
    46201 cgttaccacc agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac
    46261 gccgtactcc gcgaccagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag
    46321 gaaataaatc aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact
    46381 acgttggcta cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg
    46441 ggcccattgg cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca
    46501 gcccttccgc ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg
    46561 ctatccagct ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc
    46621 tgaccatatg atgtacaaag gcattcgctt tgatatcaac caccacataa ggaccgtgac
    46681 gcgtgacatt aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac
    46741 ggaacgaggt gaaatcattc tcgcccagca agcactgcgc agcccgatgc atccgttcag
    46801 cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat
    46861 gattgtagat gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat
    46921 caggtacagc tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca
    46981 acgtccacgc agcgtctttg cgctgcgcgg tagtttcgaa atgcacaacc tgcccggtac
    47041 cgtgtacccc tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct
    47101 gggagagcgc cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc
    47161 cgtaatactt actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcggttgtt
    47221 gctggtcgga cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat
    47281 cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat
    47341 accgtagtca ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcacctac
    47401 ctgagttggg aattcgttct cttcagagag cacaatatct tccccttgaa caaacgcatc
    47461 acgcgcttct tctgctgcca gtggacgcag agcttcaaag ttgaccatct gggcatgacc
    47521 gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc agtccttcgt cctgcaggat
    47581 tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcag
    47641 taacggcagc atgttgaacg cgagctgacg cccgaagaaa tcttcttcgt caatcggaat
    47701 gccgttgagc aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc
    47761 ggaggctgaa atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg
    47821 tttcagtgcc gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt
    47881 gcggtaatcg gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc
    47941 aagagcaaac aatccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac
    48001 ccaggctgcg gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc
    48061 gaattcagca gcatcctgca cggtgattgt cttaccacca aagcgcaatt gttcgcctgc
    48121 gctttcgtta cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt
    48181 ttcaagcagg gcttcgccca cagcgccagt tgtgcccagg acggcaatgt tccagccttc
    48241 agacatggtg gtttactcca gaaatagcaa agctccctgc caatgttata gcagagagca
    48301 tgaagaagag attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat
    48361 gatgccgcac tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga
    48421 tagtttttgc gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg
    48481 tcatcgcggc gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc
    48541 ggtccatgca gagtaatgcg accaaactct ggcgcaggca gtaatgtatc cagcgcaacg
    48601 tgctgttcat gcccaataaa cttgctatag gcttcaaaca cttgcgtagt accgcgtgct
    48661 ttaccttcca gggtatagcc tgcaatatgc ggcgtgccga tatccacttt tgtcagcaat
    48721 tccacattta gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct
    48781 tcattcaggc aggtcagcag cgcggtatta tcgacgactg cgccacggca ggcgttaatc
    48841 agaatcgctc cgggcttcag gctacggatc agtttttcat ccgccagatg cagcgttttg
    48901 tacggcccgt ctttgaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac
    48961 tcatccagcg agcggaaatc accctcatcc ccacagtcgg cgcgaggcgg atcgcaaagt
    49021 aaggttttaa tccctaacgc ttccagtcgt gcctgtaaac gccgaccaac gttaccaacg
    49081 cctacgatcc ccacggtacg ctcgtgcagt gaaaatccat cgcgttcggc aagcatcagc
    49141 agggaggaga aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa
    49201 ccaattcccg cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtg
    49261 ccaacaaatt taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc
    49321 accatcagcg catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg
    49381 atcacctcac ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg
    49441 attttcacgt ttgtgttacc tgtatgagac gagagttaac cggacaagtg tgccataatc
    49501 tcgcggccag gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat
    49561 ttccggccct cctcctgggc aaccaccagg tcagggagat aacctgccgt ctggcgcggg
    49621 caatcagcct ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac
    49681 ctcactgacg caacagcaaa gagcagaact gtgggcgggt atcaggcacg atattggtct
    49741 gtcgggagat tcaccgctgc tttcgcgtca ctgccctgcc gctgagcata atctggcgca
    49801 acgtctgctg gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga
    49861 gtatttacgt ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg
    49921 tcagacgccg ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca
    49981 ggaagggaag atggttattc cgcaaccgca gcagcgcgag gcgaccgatc gtcctctatt
    50041 accggcggag cacaatgcgc tcaaacagct ggtgaccaaa cttgcggcgg caacggggga
    50101 gcccagcaaa cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt
    50161 aattccagcg aaactgttta accatctggt gacctggctg caggcgcgtc agacgctaag
    50221 ccagcaaaat acgccgacgc tggaatcact acaaatgacg ctaaaacaac ctttagatgc
    50281 cagtgaactg gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc
    50341 atcgctttct tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa
    50401 agggattgat ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac
    50461 gttgcaaaat atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat
    50521 gctggtctgg ctggttcgtt aaccccgacg aaacgacagt atcgtgacaa taattcccag
    50581 caccgcagag atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat
    50641 tcccgcaagc ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag
    50701 tgcggtgccg cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa
    50761 gataagcgaa catccggctc cggttaacgc cgcgcccgct aatgcgaccc acgcccctgg
    50821 ggcttgccag agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt
    50881 cacgccgcca aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc
    50941 gccaaacgcg gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga
    51001 aacgaaagtc ccgataaccg caaaaccaac gccttgtagt gccagaccta accctggttt
    51061 ccagataagt ccgacaacgc tccacagaga tggacgttct cccgccaggg ctggtacttt
    51121 gcgcactgtg ccgttacagg cccacgccag taagggtaat gccattgtgg tgagcgccag
    51181 tgcggcaaaa ccgtaatggc tatgaatcaa caggccaagc ggagcaccaa cagcgagggc
    51241 accgtaaatc gccattccat tccatgacat cactttgcca gagtgttttg gccctacgat
    51301 gcctaacccc caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa
    51361 acgcccgatg accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag
    51421 cagcaacgcg ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc
    51481 gccatattga tcggccagtc gcccggcata gccacgcgtc agcaccgtag ccagaaactg
    51541 aatgccgacg gcaatgccga ccatggtatt gccatagccc agatcatgat gaacaaacaa
    51601 cgggataacc ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagcgat
    51661 gcggaagagc gaaaaattgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca
    51721 ttactccaga atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg
    51781 ttacagtgcg ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg
    51841 ggagtttgcc tggaactggc ggcgattgtc aatgatgtga aaaagggaac catcaggttc
    51901 ccttttgcgt tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg
    51961 tcttatccga cctactccgg cctactgcga attaatcttt cagcttgcgc attaccagcg
    52021 tggcgttggt gccgccgaag ccgaagctgt tagacataac agtggtcagt tcgcgatcgg
    52081 tcgtttcggt cacgatgttc agacccgcag cctgctcgtc cagctcttca atgttgatgc
    52141 tcggggcgat aaagccgtgt tccagcatca gcagagagta gatagcttcc tgtacgccag
    52201 cagcgcccag agagtgaccg gtcatggctt tggttgcaga aatcgccggg ctcttatcgc
    52261 cgaacacttc acggatagct gccagctctt tcacgtcgcc aaccggagtc gaagtgccgt
    52321 gggagttcag gtaatcgatt ggggtatcaa cgccatgcat cgccatctgc atacaacgta
    52381 ctgcgccttc gccagaggga gcaaccatgt ctgcgccatc agacgttgcg ccgtagccaa
    52441 cgatttcagc atagatgtga gcaccacgcg ccagcgcgtg ttccagctct tcaaccacca
    52501 ccataccgcc gccgccagcg ataacgaaac cgtcacggtg agcatcgtaa gtacgggagg
    52561 ctttttccgg ggtgtcgttg tatttagtag acagcgcacc cattgcgtcg aattcgcaag
    52621 ccatttccca gcacagctct tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt
    52681 ggatctgctc tactgcgtta ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg
    52741 agtagttaac gccatgaatt ttaaacggcg tggcgaggca ggcagaaacg ccggatgcca
    52801 tcgctttggt gaccacatac gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt
    52861 cagcgccgaa cacctggaaa cgcggggaac cgccgccaga acctgcaatc aggccaacac
    52921 gcggattatt ctggtaagct tccggagaga ggcccgcatc tgcgattgcc tgctccatag
    52981 aaaggaatgc ataaatggat gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc
    53041 cagtggtatc cagttttacg ttgccccaga cgtggctacg catgccggaa tccttcagct
    53101 cctgagagaa agtgatccct gaacgtcctt cacgcagaga tgccaggact tcctgctggt
    53161 tattaccgat gctggaaaca atgcccaggc cagtaatcac tgcacgtttc attcaatacc
    53221 tctgtaagtc gcacatagag taagtttcga atgcacaata gcgtacactt gtacgccgaa
    53281 caagtccgat cagccaatta atagagaaat ttgcgcagcc ttacacacat cgctaagatc
    53341 gagctaccgc ctgtaagacg agtaacttac gtgaaacact actccataca acctgccaac
    53401 ctcgaattta atgctgaggg tacacctgtt tcccgagatt ttgacgatgt ctatttttcc
    53461 aacgataacg ggctggaaga gacgcgttat gtttttctgg gaggtaacca attagaggca
    53521 cgctttcctg aacatccaca tcctctgttt gtggtagcag agagcggctt cggcaccgga
    53581 ttaaacttcc tgacgctatg gcaggcattt gatcagtttc gcgaagcgca tccgcaagcg
    53641 caattacaac gcttacattt cattagtttt gagaaatttc ccctcacccg tgcggattta
    53701 gccttagcgc atcaacactg gccggaactg gctccgtggg cagaacaact tcaggcgcag
    53761 tggccaatgc ccttgcccgg ttgccatcgt ttattacttg atgaaggccg cgtgacgctg
    53821 gatttatggt ttggcgatat taatgaactg atcagccaac tggacgattc tctaaatcaa
    53881 aaagtagatg cctggtttct ggacggcttt gcgccagcga aaaacccgga tatgtggacg
    53941 caaaatctgt ttaacgccat ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt
    54001 acgtctgccg gttttgtccg ccgcggtttg caggaagccg gattcacgat gcaaaaacgt
    54061 aagggctttg gacgcaaacg ggaaatgctt tgtggggtga tggaacagac attaccactc
    54121 ccctgctcca cgccgtggtt taatcgtacg ggcagcagca aacgggaagt agcgattatc
    54181 ggcggtggta ttgccagcgc attgttgtcg ctggcgctat tgcgtcgcgg ctggcaagta
    54241 acgctttatt gcgcggatga agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg
    54301 ttgtatccgt tattaagcaa acacgatgag gcgctaaacc gctttttctc taatgggttt
    54361 acttttgctc gtcgacttta tgattcatta cccgttaaat ttgatcatga ctggtgcggc
    54421 gtcacgcagt taggctggga tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg
    54481 gatttacccg aagaactggc tgtagccgtt gaggcaaatg cggttgaaca aattacgggc
    54541 gttacgacaa attgcagcgg tattacttat ccgcaaggtg gctggctgtg tccggcagaa
    54601 ctgacccgta atgtgctgga actggcgcaa cagcagggtt tgcagattta ttatcaatat
    54661 cagttacagg atttatcccg taaggatgac tgttggttgt tgacgtttgc aggagatcag
    54721 caagcgacac acagtgtagt ggtactggcg aacgggcatc aaatcagccg tttcagccaa
    54781 acatcgtctc tgccagtgta ttcggttgcc ggtcaggtca gccatattcc gacgacaccc
    54841 gaactggcaa aactgaaaca ggtgctgtgc tatgacggtt atctcacgcc gcaaaatccg
    54901 gcgaatcagc accattgtat tggtgccagt tatcatcgcg gcagcgaaga gacggcgtac
    54961 agtgaagaca atcagcagca gaatcgccag cgattgattg attgtttccc acacgcacaa
    55021 tgggcgaaga cggttgatgt cagtaaaaaa gaggcgcgct gcggtgtgcg ttgtgccacc
    55081 cgcgatcatc tgccaatggt aggtaatgtt cccgattatg aggcaacact cgtggaatat
    55141 gcgtcgctgg cggagcagaa agataaggcg gtaagcgcgc cggtttttga cgatctcttt
    55201 atgtttgctg ctttaggttc gcgcggtttg tgttctgccc cgctgtgtgc cgagattctg
    55261 gcggcgcaga tgagcgacga accgattccg atggatgcca gtacgctggc ggcgttaaat
    55321 ccgaatcggt tatgggtgcg gaaattgttg aaaggtaaag cggttaaggc cgggtaatct
    55381 gctctcgcag tgtttgccgg atgcggcgtg aacgccttat ccgacctacg tgtgacgtgt
    55441 aggcctgata agacgcgata agcgtcgcat caggcatggt gctccagacg ccgcaactta
    55501 ctgctgtgac gcctgctgaa acaggttttc ccacatatcg gtcaccagcg cctggtcacg
    55561 cggcgacaat tcaccagcac caatggcttt ttccagactc tggctaacgg tcgtatgcac
    55621 cgcctgagcg gagtggtcat caccactttc cagttctgcg atggctaatg taaggtggcc
    55681 acgcaaatac ccactggcaa acaactcatc atcactggca tggtcaacca taccgtcgat
    55741 taatgccaga atgcgtgatt caaactccgc gatcatcttc tttcctcatt gtaaaacgtg
    55801 gcgctggctt cagttgagcg cttccggcca cgggaactgt tccgccgtaa gttccggcgt
    55861 gtgataataa ttttgtagtg ctttaatgaa acgtgccggg cgttccggaa tgccatttgt
    55921 tagataatcc atcacctgcg catgtactcg ccgttgaaac accacgcggt ccggttcgaa
    55981 gtcgccttcc agattgtcgc aactgacgtt aaacgggtat cccgccgcca cgcagaacaa
    56041 ccagtcgagc gcctgcggct tcacttcaac atcttcaaac tggctttgcg tttgggcatc
    56101 gcgaccgtcc gggcaatacc agtagccgaa atcaacctgc tcgcggcgcg ctttcccggc
    56161 aatacaccag tgcgaaatct catgaattgc gctggcataa aagccgtggg caaagacgat
    56221 tcggttatac ggtacttccg catcagcggg aagatagatc ggttcgtcgt cgcctttaat
    56281 cagacgggta ttaaaatcat cggcaaagca gctattaaaa atttcaatca actgctcata
    56341 gtggtgtgta ctgttcatta gttcatcccc aaccagtgga ggatctcctg tccgtggcta
    56401 tcataaagta atttggcact catcaccgcc gagacgataa cgatcatcgg gcggatcagt
    56461 ttttgtcctt tgctcaacac cagtcttgaa cccatgcgcg cgccaaggaa ctgccccacc
    56521 agcatcacaa agcccgtcgc ccaaatcact ttgccgccga gaataaacag cagtaaaccg
    56581 ccgatgtttg acgttgcgtt gagtaatttg gcgtgagccg tggctttggc gaggttgaat
    56641 ccgcacagcg taacaaaggc cagcgcgtaa aacgatccgg ctgccggacc aaagaatccg
    56701 tcataaaaac cgacgcagcc accggcaatt aacgcgaagg gcaaaccgta catccggcgc
    56761 tggcgatctt cttcacccag ctttggcatc agcaagaaat agaggccgat acaaatcacc
    56821 agaatgggca aaatctgccg taagacatca gcctgaacgt actgtaccaa cagtgcgccg
    56881 ctcattgagc cgacaaaggt catggcaata ttaagtttct gatcgcttaa actaaccact
    56941 ttgcggcgaa taaagtagat agtggcggaa atagagccgc cgcaggcttg cagtttattg
    57001 gttgccagag cattagcggg agacatcccc gctgccatca atgccggaat ggtgagtaac
    57061 ccaccgccac cggcaatcga gtcgataaat cccgccagca tggcgacaaa aaagagaact
    57121 cccagcaaca gcggggaaac cataaacagg ctattaaacg tttccattag atcacgtgct
    57181 catccagtag cgcctggcag gaaggcggca acggaggcgg tgtcttcttc tcaggctttg
    57241 ttgttcccgg ttttggaggt tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg
    57301 gtggcggtaa aggttgatct tcacactcca gactatcggc aggacaacgt aatcgtacat
    57361 gcatatgcgc acgatgctgg aaccagggtc gcactttgcg caaccagtcg cgatcggtgc
    57421 ccgcatcaag gcaaagttgt tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt
    57481 tgtcctgggc ggcgagtttg atcaagctga aaatttctgg cttccacagc gtggagacaa
    57541 cgtgtttacc gtcgcgggaa actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg
    57601 tccggcgtgt tttcggcagt tgcagaaaga tatcgacatc cagtccggtc tggtggctgg
    57661 cgtgaccgcc gttgaaacgc ccaccagcag gcattcccat atcgccaatc aacaccgtac
    57721 ccatgcccag attgctcacc tgactactca ggcgctggat aaacatcacc aggtccgggt
    57781 gaccgaaata gcgacgctga tcggtacgca tgacctgata atgttcggac tgtatcggca
    57841 acgtgtcagc gccgacgata caaccattgg aaaaactgcc tatcgattgt gcgctacccg
    57901 gcacaggttg ggttattttt tgccacggcg tcgctgccag gctgacgcta ctggcaagca
    57961 gggccagcag cgcaatcgcg gttttgttca ttttttacca gcgtggaata tcagtcttca
    58021 catcggcatt ttgcgcccgc tgccgtaaca ggtgatccat taaaacgatc gccagcatcg
    58081 cttctgctat cggcaccgcg cggatcccga cacagggatc gtgacggcct ttggtgatca
    58141 tctcaacttc ttcgccaaag cggttaatgg tgcgccccgg cacggtaata ctggaggtcg
    58201 gtttcagtgc catatgagca atgatttgct gcccgctgct aattccgccg agaatgccgc
    58261 ccgcatggtt actctggaaa ccgtctttgg tgatttcgtc gcggttctgg ctgccacgca
    58321 gcgccaccac gtcaaaacca tcaccaattt ccacgccttt caccgcgtta atgctcatta
    58381 gcgcatgggc gatgtcggca tccaggcgat caaagaccgg ctcaccaagc ccggcaggaa
    58441 cgccactggc aacaacggtg actttcgcgc caatagagtc gccctctttt ttcagcgcgc
    58501 gcatcagttc atctaacgcg tcgattttgt ccgggtccgg gcagaagaat ggattttgct
    58561 cgacctgcga ccagtctttg atttccagcg gaatgtcgcc catctgggtc aggcagccgc
    58621 gaatttcgat gccaaatttc tcagcgagat attttttggc aatcgcccct gctgcaacgc
    58681 gcatggcggt ttcacgggcg gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt
    58741 tttgttcgta ggtgtaatcg gcatggccag gacggaaaac gtccttaatc gcactataat
    58801 cctgagaacg ctgatcggta ttttcgatca acaaaccaat gctggtgccg gtagtaacac
    58861 cttcaaaaac accggagaga attttgacct gatctggctc acgacgctgg gtggtatagc
    58921 gcgatgtccc agggcgacga cggtcgaggt catgttgcag gtccgcttcc gtcagcggaa
    58981 tgcctggcgg aacaccatcg acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg
    59041 tggttacgcg aaagagttgt ccaattgtgt ttccagccat cacggctccg ttattgttgt
    59101 gtttgcgtgt ttacttaatc tttataaatc gcgaaatgtt cacgggccgc cagcagctgc
    59161 gctttggtga gcataaacac accatcgccg ccgttatcaa actccagcca ggtgaacgga
    59221 acatcgggat attgttccat aagatgtacc atgctgttgc cgacttcaca aatcagtacg
    59281 ccatcatcag caagataatc tgccgcgttt ccgagaatgc gacgcgtcag tttcaggccg
    59341 tcggtgccag atgccaggcc cagttccggc tcgtggcggt attcatttgg caggtcggac
    59401 atatcttccg catcgacata cggcgggtta gtgacaatca ggtcgtactg cactttcggc
    59461 aaatcacgga acagatcgga acgaatcggt atgacgttgt ggatcagacc gtgttcttcg
    59521 atgttctgtt cggcaaccgc cagcgcgtcg ggagagatat ctaccgcatc gacttccgct
    59581 tccgggaagg cataagcaca ggcaatggcg atgcagccgc taccggtaca catatctaaa
    59641 atatgctgcg gttgcttgct gataagtccg gcaaatttat tgttgatcaa ttcaccaatc
    59701 ggcgagcgcg gcaccagcac gcgttcatcg acgtaaaatt catggccgca gaaccacgct
    59761 ttgttggtca ggtaagctac cggaatgcgt tcgttgacgc ggcggatcac gcgctcaaca
    59821 atacgatgtt tttcgctgga ggtcagacgc gcggtgcgca tatcttccgg aatatccagc
    59881 ggcaggtaga gcgacggcaa taccagctgt acggcttcat cccacgggtt atcggtgccg
    59941 tgaccgtacc agatatttgc cgcgctgaag cggctcaccg accagcgcaa catgtcctga
    60001 atggtttgca gctcatttac tgcttcatca acgaaaattt tatccacgta ttcctccagg
    60061 gcatgatcgc aataatttcg gcggctagtt tgccatgaag atgacgataa atcagcattc
    60121 acgcgcggtg agtgaggaaa aatacgttta aaacgatcga ttgcgctacg agtcgggtaa
    60181 actgtaggaa aattagaaat agagacagat aaatgaaaaa gaaaacaaca cttagcgagg
    60241 aggaccaggc tctgtttcgc cagttgatgg cggggactcg caagattaag caggacacca
    60301 ttgtccatcg gccacagcgt aaaaaagtca gcgaagtgcc cgtcaaacgg ttgattcagg
    60361 agcaggttga cgccagtcac tatttctctg acgaatttca accgttatta aatgctgatg
    60421 gcccggtgaa gtatgtacgc cctggtgtag accattttga ggcgaaaaag ctgcgccgcg
    60481 gcgattattc gccggaattg tttttggatt tacacggtct gacgcagctg caggccaagc
    60541 aggaactggg cgcgttgatc gccgcctgtc gccgcgaaca tgtgttttgc gcctgcgtga
    60601 tgcatggtca cgggaaacat atattgaagc aacaaacacc gctgtggctg gcacaacatc
    60661 cgcatgtcat ggcgtttcac caggcaccaa aagagtatgg tggtgatgcc gcattgctgg
    60721 tgttgattga agtggaagag tggctaccgc cggagttgcc atgaatatta gggggcggat
    60781 gcgttttgcc atttcgccct gaatgctggt atcgaatgcc ggatgcggcg taaacgcctg
    60841 atccggctta tcaaaatgtg caaattcaat acgttgtgat tcagttgtag gcatgataag
    60901 acgcgtcagc gtcgcatcag gcatcaacgc acaactacag ttcattcagt acatactgga
    60961 aacggcatgt agaatcaatt ggctcacggc tactggtatt cacctgtacc acaatgtcgt
    61021 gtagctttct atccgtcaat ggttgacgtg agggggtgac tgtcaccttt gcattatcac
    61081 aaccatacga ggtaaaggtt aataagtcag aaacagcacg acaatatgcc cctctggaat
    61141 caaaaatact gccatcgcag agtttgccgc tttccatcag gtaggtcgaa agcgtagtcg
    61201 tgattgtcga accatcggat tgcaatcgca gcgctttatt atggctaaca taattatcac
    61261 tccaggatat atcaaacgaa gcgacgggaa ttgtcgggca aatagaatca tgaactgttg
    61321 ggcttgtata cgtaccaaat ccaaatgcct cagatactga ataaaatgag gcaatccacg
    61381 gacaaccata attatcagat tctacagtga cgttaaaagt catatatgca cagacaaaga
    61441 cacgagtatt gataccaggg caatttgagg tgtccgtacg tctataatca gtcaaaacca
    61501 gtgtcggtgc cgggccagat ccgctccagg atattaactt gagggtgtcg ccttttcttg
    61561 cattggagtt gtattgtccg tagcttactt ctcccggcgt cactaccgtg acatagaaat
    61621 ataaactgcc ggaagcagta acgtttttca tcgcataata aattttagta ctggaaaagc
    61681 ttgcactcat gagtttatca ccggcatagc actttggtgt ttctaaactg agagcaagta
    61741 atatcagact tgtcaaaaat atcgtccatt ttttcatttt aaaaatactc aaaagttacc
    61801 gtcattgtgg cggtgaaatc acccgatgta acatttcgtc catttacggt ctgtaaccag
    61861 gcattaaaat tgacactgtt atttccctga tttaatggaa atgaggcacc tgtggtactg
    61921 ttaataggta cagccgcccc tccggcagtt tcaatgccaa tcccaacacc cgttgcagaa
    61981 gatgacgaat caatcgacag aaatcctggt aagtcggtat cttctgttcc catcaaggtc
    62041 accttgacat taaacgccgt ggtgcttttg caatctttca actgaaaaac taacggtacg
    62101 cgatctgact gaccacgttg cattaaatcg ctggcagcaa ttgtggggaa atgaatttct
    62161 gcaagtaagt tgccattgat tacaggagta caggatttac caagcaaatt accggtaaaa
    62221 tgcaggttat cggcagctgg ggccgttccg ctgaacaata tcagaccact gataagcgcc
    62281 cacgcacggt tattcttcat tggtaatcca cctgtaacgt agcccatgct tcaaagtctg
    62341 actcgggtaa gtcgacaccg ctctttttta cgggtactgc cgttagtacc ggaagggatg
    62401 tttcattcac cactaagggc gtatgcagct taaaatcgct cccggcttgt ttgaggtgta
    62461 ttcctaatcc attgagatcc gtggctacgg ccgaatcgtc aaagtcactc tgcgtaccac
    62521 tccaggtcaa cgtcatttcc atttgagggt cacggacagc ggtatcgcag gtaatggtat
    62581 acggcacgac ctgttcgtaa ttgctaccgt taatgtcatc aatattcacg tcccgaaacg
    62641 tcaactctat attttgccca ttactaatag agcaattggg tggctgcact aaataaccat
    62701 gaaaagagac attcgagagc gccataaagc taaagctcca caacattaag agagagatcc
    62761 gtttcattga taatccacca caattgtggc actggcgcta aattccgccg ccgttagcga
    62821 tacaccgctc tgcacgacag gaaccgcttc cagagccagt gcgccagatg aaaagttaaa
    62881 cggtaaccat gcgccaggcg ttaaatccaa aatggagtga tcgcttactt tctgaatacg
    62941 gataccaaaa ccggcaatac ccgtatcgat taccgtttct ccgttaatta cggttgtggt
    63001 tgcctgcata tttaagcgca ggtcgtccat tgccagattg ttgcacacaa gcgagtagtc
    63061 aatggggcgt ttgtaactta ccccatcgat cttggaaatg aagacattac caaactccac
    63121 gctcgcaccc gttaccgtac agggcggcgg ggcatcaacg agcacacgca agaccatcgc
    63181 aaccgattta ctccccgcca ttacaggcgt gctcacaatg ctacacagca ataaccacgc
    63241 ccaggccgaa taagctcctt tcatgctatt gctcctatcc ctggcctttg ctgttttgct
    63301 cttcgtccac ctgacaggta tttcccgtac agcggaagaa cagcggcaaa cgcgcaccat
    63361 aatcattcac ataggtcagc accgggacgc tatccatttt gacttttaac ggcacagagg
    63421 tttttggcga tatcaccatg ggggaaaatc ccgctgccgg attgccattt ttctgcgcac
    63481 tggcgttact gataaccacg aagtacggcg tcgggttatt gacggtaaac tcctgaccac
    63541 tgagggttaa ggtcacctta tgctgccacg ggtttttcat gctgacgttt tccagcgcct
    63601 ttggtcgcca gaacaactta atgcgcgtct gcagcgctat ctgtagcgtg ttaggtttgt
    63661 tcgatttcgg cgggatctca cgcacgttga aatagaacac gctctcgcgg tcggcaggca
    63721 gcttgttgat atccggcatg ccctgtacct tcacctgacc gttcatcatg gaatcgatgc
    63781 gctgcactgg cggcaggacc gtcagtggcg agctaatcct gttaccctgt ccatcttcca
    63841 tccagctttg tgccagatac ggcattttcg ggtcgttatt gcgcagcgtg acgctgattg
    63901 atttatcact ttcgttaaaa atcaggcgtg tacggtccgg cgtgacagag gcctgtactg
    63961 acaggctggc ggcagttatc agtaacgcca gcgtcgttgc ggccgttctg gcagagcaaa
    64021 atagattcgt catggatcat tccttaatca cgttattaat tggctgatac cgatactggc
    64081 gcttccgttg gcatcacctg ctgggtctgc tcctggatca ccggcttaat ttcatgaggc
    64141 atcgaaggag atatcgcccc tgtttgctgg catggtaata acaggccatt gaacagatcg
    64201 gctggcagcg ggtcaggcag atgaatatcg cagtgggcca caccgcccca tgaaacgatc
    64261 atatgctcgc caggttttac gcccgcgagg tagacgttgc cgtcatcgtc aaccagaccg
    64321 acgttctgcg cgctgtcgtt tttcacttcc gcgccaaacg gcggataact gccatcttgc
    64381 agacgcagca ccgccatcgc tttttgcccg ctgatcaccg agaacttacg gtagccaatg
    64441 gccccttcgg taagcgtggc ctgcaccacg gactgggtgg cttcggcgtt ttccggcagg
    64501 ttgtttaggt cgatatacgc ctggttgcgg tagtagttgt tgacgtctgc caccactgcc
    64561 ttaccgaaca tattggtgta aaccgccgcg ccatttcctt ccaccggaac accagccaca
    64621 ccgtcggcat caatcagcag acgcgtaccg cccatattct gggtacggtg gagcgcgcca
    64681 ccttgtgcgg tgagcgtcgc gccgccctgt aaggaaatac ccgccgaggt gtactgccct
    64741 tcatggtagt tagcgctgag atcgacctgc gccagcgatc cgtcgtggct gtaataaccg
    64801 tcaacgctgg agtgattgtc gctggtgcct acgttcaact ggtaatgggt tgcgtcatcg
    64861 acacggctga aataccccac ctggctgctg tccgaaccgc tgccgtagtt gccgttatag
    64921 ctgatggtgc tgctgtcacc ccacggcata ctgagcgata tgtacacacc tttatcggcc
    64981 tggttgtcat actcgtagcg gtatccggtc atggaaatgc tcatgttgcg gatactgccc
    65041 aggttgaagt agtgcgagag catgacgttg tagttggtct gttcgtcgcg atcccagtag
    65101 gtatggcggg tgtagttgag ataaacagac acaccggcat ccctgaagtt ctggttatag
    65161 gtggcggtgt acatctcttt gtcgttgccg gtgcgcacca tttcgctgtc gctggcatcg
    65221 agatactcgc tcatggtcat gaagttctct tccgagaagc ggtatccggc aaaagtgacg
    65281 cggctgttca gttcatcgaa gtctttggaa tagctcaggc gaaacgagtt gccgtccagt
    65341 gaacctttcc cgtaggcggt ctctttatcc agacgggtat gcgagtgggt gatatcaaag
    65401 gccaccgcac caaacacaga caggtcgcga ccgacgccaa gcgccgccga ctgatagtgt
    65461 tcatctgcca gcgccccgcc gtatagcgac cagccgttgg caatccccca ggaagcttcg
    65521 ccgccggaga agaaaccgcc ttccacgtgg tgcccccact cctgcgggcg gcccatcatc
    65581 agtttatagc gcacctggcc ggggcgagtc aggaacggca tcgaggcggt gttgatgtca
    65641 tattcctgca cctgaccgtt ctgttcttca atgcggatat gcagcgtacc ggagaccgaa
    65701 tcgccaagat cctggatgcg gaacggcccc gccgggacct gggtttcgta gatgacgcgg
    65761 cccaactggc tgacggtcac tttcgcggtg gtgtgcgcca cgccggagat atccggcgca
    65821 tagccgcgca gattcggcgg caacatttga tcgtcggtgc tgatactgcc acccacgtag
    65881 ttaaagccgt cgaaaatatc agaattcagg tagtcttcgc caaggccaag tttggctttt
    65941 agcgacggta aggctcgcca ggcgtagtag cggctccact cccagttttt ttgcgtgtca
    66001 tcaccgttga taacatcgtc atcattgctt ttactatgca aataatcagt ctgccagtcg
    66061 gcacgaagac gccatgcgcc gaggttcacc ccaaccgtcc cgttaccgct aatttcattg
    66121 ctgtcatccc cgccattttc ttcatgtcgt gtctgggcgg taatactgta gtcagcaatt
    66181 aaaccagata taccgtcatc ccagcgtgaa ggcggatccc agttgatgtc ggtatattca
    66241 aggtaagcct ggggtaatga aatgactaac gctgactgac tcaggtcagc tttaatatca
    66301 atgccttcca gttgaccggg tttcaggcat ttgccgtcgt ggatccattg caggtttttt
    66361 gccacatcct cttttaagcc aaactgcgcg accagttcag gcgtcaggca ggcataggtt
    66421 ttactggcat cgttctcaga ggcgtaccag taaatatcgt attcttccgt cagcggctgt
    66481 ttatttagtt gaacctgtaa attgtatttc ccaggttcga cataaccctg gctggaaaat
    66541 cgcttcagat cgatttttgt gtcgcctttt aactccagaa aacgggaatc aaactgaatt
    66601 tcattttcag cccaggcatt gacataactc ccggacattg ccaatgcaac gcagcaggga
    66661 agtacccgca gtcggaaaag agaatggtca ggcatacacg tatccatccg taattattat
    66721 aatttgcgcg aattccattt aaaaacccca tagcccgaaa gctatggggg tatcacttag
    66781 agataagtaa tctggaaggt cgtgttggct tcaaaattac ctaaatctgg cgcatcagca
    66841 gcgcccacca gccaggcttt aaagtccagc gtctgcttcg ctttgccttt gttggtcgcc
    66901 gtatcgttta cgattttctg ttcgataccc gcgccgcttt tataagaggt tccctgagcg
    66961 tcaccaatgg ccaggctgac attgttaaat gccgcaccag tatcggtatt gtaaatggtg
    67021 tagtaattgc cgctgttggt agaagacgcg ttaccggtga aggtagtggt catcgttgtc
    67081 tgggtgtcaa acacacagtc ttgcagatgg atctggaaag ctttcggtgt agaagtcccg
    67141 ttatttttca ggatccctga accaatgtca cccatatcaa cttccagttt gtgatcatca
    67201 ggaacgatag agcacgggga atcttcaatg gtgccgtaga aacgtgctgt accattgttg
    67261 ccagcggcga ttgttgaagt agaagcgaac gcacccatta ccattgttgc agcaatagct
    67321 gtttttacaa acttactcat tatttgtcct taaaccataa ataattaaaa atataaacat
    67381 tgcaatacat tgattcagtc aatagcaatt atttataccc agccattgaa taagctatcc
    67441 gatctaacta acaagaaaat aggtcgcata agaagatatt ctttggtgtg ggcggatggt
    67501 gatattttgt ctttattgtt tca
//
LOCUS       DAZFDC010000010        65464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFDC010000010 DAZFDC010000000
VERSION     DAZFDC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.010"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(76..1341)
                     /locus_tag="ACHJ48_000763"
     CDS             complement(76..1341)
                     /locus_tag="ACHJ48_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4706378.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALVVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
     gene            complement(1539..1614)
                     /locus_tag="ACHJ48_000764"
     tRNA            complement(1539..1614)
                     /locus_tag="ACHJ48_000764"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Phe,seq:gaa)
     gene            complement(1720..2418)
                     /gene="yqgA"
                     /locus_tag="ACHJ48_000765"
     CDS             complement(1720..2418)
                     /gene="yqgA"
                     /locus_tag="ACHJ48_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO4706379.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            2571..2654
                     /locus_tag="ACHJ48_000766"
     CDS             2571..2654
                     /locus_tag="ACHJ48_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706380.1"
                     /translation="MHAISLHSLAYRRFARNPSLFMLLLMR"
     gene            2816..4951
                     /gene="speC"
                     /locus_tag="ACHJ48_000767"
     CDS             2816..4951
                     /gene="speC"
                     /locus_tag="ACHJ48_000767"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO4706381.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5001..6257)
                     /gene="nupG"
                     /locus_tag="ACHJ48_000768"
     CDS             complement(5001..6257)
                     /gene="nupG"
                     /locus_tag="ACHJ48_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO4706382.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6459..7538)
                     /gene="mltC"
                     /locus_tag="ACHJ48_000769"
     CDS             complement(6459..7538)
                     /gene="mltC"
                     /locus_tag="ACHJ48_000769"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO4706383.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGGTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7603..7878)
                     /gene="yggX"
                     /locus_tag="ACHJ48_000770"
     CDS             complement(7603..7878)
                     /gene="yggX"
                     /locus_tag="ACHJ48_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO4706384.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7906..8958)
                     /gene="mutY"
                     /locus_tag="ACHJ48_000771"
     CDS             complement(7906..8958)
                     /gene="mutY"
                     /locus_tag="ACHJ48_000771"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO4706385.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9119..9838
                     /gene="trmB"
                     /locus_tag="ACHJ48_000772"
     CDS             9119..9838
                     /gene="trmB"
                     /locus_tag="ACHJ48_000772"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO4706386.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMIEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9838..10164
                     /gene="yggL"
                     /locus_tag="ACHJ48_000773"
     CDS             9838..10164
                     /gene="yggL"
                     /locus_tag="ACHJ48_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO4706387.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERNLDEVRTSEL
                     FDVWWD"
     gene            10348..11067
                     /gene="yggN"
                     /locus_tag="ACHJ48_000774"
     CDS             10348..11067
                     /gene="yggN"
                     /locus_tag="ACHJ48_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO4706388.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11243..12289
                     /gene="ansB"
                     /locus_tag="ACHJ48_000775"
     CDS             11243..12289
                     /gene="ansB"
                     /locus_tag="ACHJ48_000775"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO4706389.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFIASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12406..13413
                     /gene="yggM"
                     /locus_tag="ACHJ48_000776"
     CDS             12406..13413
                     /gene="yggM"
                     /locus_tag="ACHJ48_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO4706390.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWTVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLETALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13656..14792)
                     /gene="hemW"
                     /locus_tag="ACHJ48_000777"
     CDS             complement(13656..14792)
                     /gene="hemW"
                     /locus_tag="ACHJ48_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO4706391.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14785..15378)
                     /gene="rdgB"
                     /locus_tag="ACHJ48_000778"
     CDS             complement(14785..15378)
                     /gene="rdgB"
                     /locus_tag="ACHJ48_000778"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO4706392.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTELGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15386..15676)
                     /gene="yggU"
                     /locus_tag="ACHJ48_000779"
     CDS             complement(15386..15676)
                     /gene="yggU"
                     /locus_tag="ACHJ48_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO4706393.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEVAALIN"
     gene            complement(15673..16239)
                     /gene="yggT"
                     /locus_tag="ACHJ48_000780"
     CDS             complement(15673..16239)
                     /gene="yggT"
                     /locus_tag="ACHJ48_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO4706394.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16257..16961)
                     /gene="yggS"
                     /locus_tag="ACHJ48_000781"
     CDS             complement(16257..16961)
                     /gene="yggS"
                     /locus_tag="ACHJ48_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO4706395.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16979..17959
                     /gene="yggR"
                     /locus_tag="ACHJ48_000782"
     CDS             16979..17959
                     /gene="yggR"
                     /locus_tag="ACHJ48_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO4706396.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLTENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGTPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(18150..18566)
                     /gene="ruvX"
                     /locus_tag="ACHJ48_000783"
     CDS             complement(18150..18566)
                     /gene="ruvX"
                     /locus_tag="ACHJ48_000783"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO4706397.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKIDSASAVIILESYFEQGY"
     gene            complement(18566..19129)
                     /gene="yqgE"
                     /locus_tag="ACHJ48_000784"
     CDS             complement(18566..19129)
                     /gene="yqgE"
                     /locus_tag="ACHJ48_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO4706398.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19241..20188)
                     /gene="gshB"
                     /locus_tag="ACHJ48_000785"
     CDS             complement(19241..20188)
                     /gene="gshB"
                     /locus_tag="ACHJ48_000785"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO4706399.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQ"
     gene            complement(20201..20932)
                     /gene="rsmE"
                     /locus_tag="ACHJ48_000786"
     CDS             complement(20201..20932)
                     /gene="rsmE"
                     /locus_tag="ACHJ48_000786"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO4706400.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(21012..21719)
                     /gene="endA"
                     /locus_tag="ACHJ48_000787"
     CDS             complement(21012..21719)
                     /gene="endA"
                     /locus_tag="ACHJ48_000787"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO4706401.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDHYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21814..22311)
                     /gene="yggI"
                     /locus_tag="ACHJ48_000788"
     CDS             complement(21814..22311)
                     /gene="yggI"
                     /locus_tag="ACHJ48_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO4706402.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYSCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22388..23782)
                     /gene="galP"
                     /locus_tag="ACHJ48_000789"
     CDS             complement(22388..23782)
                     /gene="galP"
                     /locus_tag="ACHJ48_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO4706403.1"
                     /translation="MPDAKKQGQSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAGLNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(24219..25373)
                     /gene="metK"
                     /locus_tag="ACHJ48_000790"
     CDS             complement(24219..25373)
                     /gene="metK"
                     /locus_tag="ACHJ48_000790"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO4706404.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            25429..25680
                     /gene="yqgD"
                     /locus_tag="ACHJ48_000791"
     CDS             25429..25680
                     /gene="yqgD"
                     /locus_tag="ACHJ48_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO4706405.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            25791..25934
                     /gene="yqgG"
                     /locus_tag="ACHJ48_000792"
     CDS             25791..25934
                     /gene="yqgG"
                     /locus_tag="ACHJ48_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO4706406.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            26028..26159
                     /gene="yqgB"
                     /locus_tag="ACHJ48_000793"
     CDS             26028..26159
                     /gene="yqgB"
                     /locus_tag="ACHJ48_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO4706407.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            26168..28144
                     /gene="speA"
                     /locus_tag="ACHJ48_000794"
     CDS             26168..28144
                     /gene="speA"
                     /locus_tag="ACHJ48_000794"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO4706408.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            28290..29021
                     /locus_tag="ACHJ48_000795"
     CDS             28290..29021
                     /locus_tag="ACHJ48_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4706409.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            29157..30077
                     /gene="speB"
                     /locus_tag="ACHJ48_000796"
     CDS             29157..30077
                     /gene="speB"
                     /locus_tag="ACHJ48_000796"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO4706410.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(30283..31041)
                     /gene="loiP"
                     /locus_tag="ACHJ48_000797"
                     /gene_synonym="yggG"
     CDS             complement(30283..31041)
                     /gene="loiP"
                     /locus_tag="ACHJ48_000797"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO4706411.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            31319..33310
                     /gene="tkt"
                     /locus_tag="ACHJ48_000798"
     CDS             31319..33310
                     /gene="tkt"
                     /locus_tag="ACHJ48_000798"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO4706412.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            33624..34067
                     /gene="cmtB"
                     /locus_tag="ACHJ48_000799"
     CDS             33624..34067
                     /gene="cmtB"
                     /locus_tag="ACHJ48_000799"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO4706413.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            34095..35483
                     /gene="cmtA"
                     /locus_tag="ACHJ48_000800"
     CDS             34095..35483
                     /gene="cmtA"
                     /locus_tag="ACHJ48_000800"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO4706414.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            35498..36775
                     /gene="yggP"
                     /locus_tag="ACHJ48_000801"
     CDS             35498..36775
                     /gene="yggP"
                     /locus_tag="ACHJ48_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4706415.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVSAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            36772..37737
                     /gene="glpX"
                     /locus_tag="ACHJ48_000802"
     CDS             36772..37737
                     /gene="glpX"
                     /locus_tag="ACHJ48_000802"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4706416.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRDVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGNMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDVLVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLY"
     gene            37759..38268
                     /gene="fumE"
                     /locus_tag="ACHJ48_000803"
     CDS             37759..38268
                     /gene="fumE"
                     /locus_tag="ACHJ48_000803"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO4706417.1"
                     /translation="MATLSEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEESVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLKEQD
                     ETLGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            38265..38978
                     /locus_tag="ACHJ48_000804"
     CDS             38265..38978
                     /locus_tag="ACHJ48_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO4706418.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWAYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPIIIVEGNWLLLDDDN
                     WRQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(38950..39627)
                     /locus_tag="ACHJ48_000805"
     CDS             complement(38950..39627)
                     /locus_tag="ACHJ48_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4706419.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNIN
                     PPDDIHP"
     gene            complement(39621..40298)
                     /locus_tag="ACHJ48_000806"
     CDS             complement(39621..40298)
                     /locus_tag="ACHJ48_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4706420.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(40286..40993)
                     /locus_tag="ACHJ48_000807"
     CDS             complement(40286..40993)
                     /locus_tag="ACHJ48_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO4706421.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALTIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(40994..41572)
                     /locus_tag="ACHJ48_000808"
     CDS             complement(40994..41572)
                     /locus_tag="ACHJ48_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO4706422.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(41595..42026)
                     /locus_tag="ACHJ48_000809"
     CDS             complement(41595..42026)
                     /locus_tag="ACHJ48_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO4706423.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            42398..43417
                     /gene="epd"
                     /locus_tag="ACHJ48_000810"
     CDS             42398..43417
                     /gene="epd"
                     /locus_tag="ACHJ48_000810"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO4706424.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            43467..44630
                     /gene="pgk"
                     /locus_tag="ACHJ48_000811"
     CDS             43467..44630
                     /gene="pgk"
                     /locus_tag="ACHJ48_000811"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO4706425.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDEILSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            44845..45924
                     /gene="fbaA"
                     /locus_tag="ACHJ48_000812"
     CDS             44845..45924
                     /gene="fbaA"
                     /locus_tag="ACHJ48_000812"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO4706426.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(45988..46086)
                     /locus_tag="ACHJ48_000813"
                     /pseudo
     CDS             complement(45988..46086)
                     /locus_tag="ACHJ48_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            46282..47142
                     /gene="mscS"
                     /locus_tag="ACHJ48_000814"
     CDS             46282..47142
                     /gene="mscS"
                     /locus_tag="ACHJ48_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO4706427.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            47281..47916
                     /gene="argO"
                     /locus_tag="ACHJ48_000815"
     CDS             47281..47916
                     /gene="argO"
                     /locus_tag="ACHJ48_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO4706428.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            48009..48749
                     /gene="yggE"
                     /locus_tag="ACHJ48_000816"
     CDS             48009..48749
                     /gene="yggE"
                     /locus_tag="ACHJ48_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO4706429.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            48916..49812
                     /gene="ygfI"
                     /locus_tag="ACHJ48_000817"
                     /pseudo
     CDS             48916..49812
                     /gene="ygfI"
                     /locus_tag="ACHJ48_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(49809..51287)
                     /gene="scpC"
                     /locus_tag="ACHJ48_000818"
     CDS             complement(49809..51287)
                     /gene="scpC"
                     /locus_tag="ACHJ48_000818"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO4706430.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHNEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(51311..52096)
                     /gene="scpB"
                     /locus_tag="ACHJ48_000819"
     CDS             complement(51311..52096)
                     /gene="scpB"
                     /locus_tag="ACHJ48_000819"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO4706431.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(52107..53102)
                     /gene="meaB"
                     /locus_tag="ACHJ48_000820"
     CDS             complement(52107..53102)
                     /gene="meaB"
                     /locus_tag="ACHJ48_000820"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO4706432.1"
                     /translation="MINEATLAESIRRLRQGEHATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(53095..55239)
                     /gene="scpA"
                     /locus_tag="ACHJ48_000821"
     CDS             complement(53095..55239)
                     /gene="scpA"
                     /locus_tag="ACHJ48_000821"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO4706433.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(55443..56336)
                     /gene="argP"
                     /locus_tag="ACHJ48_000822"
     CDS             complement(55443..56336)
                     /gene="argP"
                     /locus_tag="ACHJ48_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO4706434.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            56719..57378
                     /gene="rpiA"
                     /locus_tag="ACHJ48_000823"
     CDS             56719..57378
                     /gene="rpiA"
                     /locus_tag="ACHJ48_000823"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO4706435.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            57633..58865
                     /gene="serA"
                     /locus_tag="ACHJ48_000824"
     CDS             57633..58865
                     /gene="serA"
                     /locus_tag="ACHJ48_000824"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO4706436.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            59094..59153
                     /gene="ibsC"
                     /locus_tag="ACHJ48_000825"
     CDS             59094..59153
                     /gene="ibsC"
                     /locus_tag="ACHJ48_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO4706437.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            59487..59546
                     /locus_tag="ACHJ48_000826"
     CDS             59487..59546
                     /locus_tag="ACHJ48_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4706438.1"
                     /translation="MMRLVIILIVLLLISLPAY"
     gene            complement(59647..60249)
                     /gene="fau"
                     /locus_tag="ACHJ48_000827"
     CDS             complement(59647..60249)
                     /gene="fau"
                     /locus_tag="ACHJ48_000827"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO4706439.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(60270..60453)
                     /gene="ssrS"
                     /locus_tag="ACHJ48_000828"
     ncRNA           complement(60270..60453)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHJ48_000828"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(60495..60824)
                     /gene="zapA"
                     /locus_tag="ACHJ48_000829"
     CDS             complement(60495..60824)
                     /gene="zapA"
                     /locus_tag="ACHJ48_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4706440.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            60992..61570
                     /gene="ygfB"
                     /locus_tag="ACHJ48_000830"
     CDS             60992..61570
                     /gene="ygfB"
                     /locus_tag="ACHJ48_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO4706441.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            61596..62921
                     /gene="pepP"
                     /locus_tag="ACHJ48_000831"
     CDS             61596..62921
                     /gene="pepP"
                     /locus_tag="ACHJ48_000831"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO4706442.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DIDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            62918..64096
                     /gene="ubiH"
                     /locus_tag="ACHJ48_000832"
                     /gene_synonym="visB"
     CDS             62918..64096
                     /gene="ubiH"
                     /locus_tag="ACHJ48_000832"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO4706443.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLEGGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            64120..65322
                     /gene="ubiI"
                     /locus_tag="ACHJ48_000833"
                     /gene_synonym="visC"
     CDS             64120..65322
                     /gene="ubiI"
                     /locus_tag="ACHJ48_000833"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO4706444.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPVKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(65396..>65464)
                     /locus_tag="ACHJ48_000834"
     CDS             complement(65396..>65464)
                     /locus_tag="ACHJ48_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706445.1"
                     /translation="KSKIRDIRKYNAKTEVKLVYFK"
BASE COUNT        15785 a        16334 c        17407 g        15938 t
ORIGIN      
        1 ctaataccgt tttgtctgtt tttttaacaa agaaaataat cctggagtag gttcccgggc
       61 atttgcccgg gatgattact gttttaatgg attattaatc tttgcatatt caaatggact
      121 gataaatctt tctctgtttg catccagaaa atcggcccac cactgtagca tcagtcgccg
      181 ttcttccaga tgttctgctt tatgaatgta agcagcacgt acaccgttac gttcctgatg
      241 gctcatctga cgttcaactg catcgcggga ccacaaacct gattctatta acgcactaca
      301 tgccatcgcc cggaatccat gaccacagac atcctgggtt gtgtcatacc ccataaccct
      361 cagggcttta tttacggtgt tttcactgat tggtttacgt gggtcatgtg ctcccgtaaa
      421 gatcagacca ttttcaccag cccatgtctg aagttctgcc agtatggcta caacctgttt
      481 gctgagtggc acaagatgtg gtgttcgcat cttggatccc cggtgtgaga attttacgcc
      541 tggcagaggt tcgcgttcgg ctggaatagt ccacatggca ttgtcaatat caatttcaga
      601 ccagcgggcg tatctgagtt cactggaacg aataaaaacc agtaaattca gcataacggc
      661 aagctgggtg acaggttgcc ccttgtaact gccaatacgc gacaaaagtt caggcaggcg
      721 agaaagatca agagcggggc gatgctggcg ttttaccgta gtcaatgcgc cagccatatc
      781 cagagcagga ttatatctga tgatgccaga ctgtacggca taacgcatta cggcagatat
      841 tctttgctgc agacgactgg cgatttcata aatttgttta gcttctgcag cacgaacagg
      901 gataagcagg tccggggtat tgagtgttgt gatatcccga ttgccgataa aggggaatac
      961 gtgtgtctcg aggcttttga gtactttatc gctgtgtgat tgtgcccatt gtttattact
     1021 ggcatgccag cgtcgtgcaa tatgttcgaa agagagttgt aaatcaggga ctactttttt
     1081 agctcttttc tgttcgcaag ggtcaacccc ctccgaaatc agttttcggg cctcatcccg
     1141 ttttgtacga gcttcggaga gagatacttc tggatacaca acaagtgcca gagtcttctc
     1201 tttacccaga atacgataac ggagacgcca gtaacgggaa ccattaggat gtaccagcag
     1261 gaacatgcca gccccgtcag tgagtttata agccttgtca gtgggctttg cagcccggat
     1321 ttttgcgtca gtcagtgcca tagttcagcc ttctgaatgc gttaaagagg gtacaaattc
     1381 tgtcgaactg gggtatgccc gcaattatac ccacacaatg aggttgatgt ggggagaatt
     1441 tggttgagtt cggtagaatt gatttggagg aagaacactt aaatcatggt gttctgaaac
     1501 gaaaaacgga cctccgtgga ggtccgttga tatgaatttg gtgcccggac tcggaatcga
     1561 accaaggaca cggggatttt caatcccctg ctctaccgac tgagctatcc gggcaacggg
     1621 gcgcattaaa cctgattcgc ctcgtctcgt caatcaaatt tcttcaattt actgcagact
     1681 gcacaatcta tcatcacttt gccgctattg cacacattgt caggcaaacc aggcggcgga
     1741 aagcggcatt gccagaagga gtgctggaag catgttgacc accgggaaca ttttaatgcc
     1801 acagatgcgt aatccggttg ccagcagcaa taaaccgcct acggcgctga agtctgccat
     1861 catcgacggt gtggtcagcg gtaatatcag cgcggcagcc cacgccagcg ttaactggat
     1921 gatcagtaat gggatactaa tcaccgatac cgcaataccc agcgagcagg cgaagatcat
     1981 cgccgtaaag aaatcaagaa atgacttggc gattaaaata ctcggatcgc cggtcatccc
     2041 ttcgttcatc gccccgaaga tcccggtgcc gctggcgcaa aacaggacaa taatcgcgac
     2101 ataattctga ataaaagatt catgcgctgg cttcttacgt gagtgacgaa acagattttg
     2161 tgctttggcg accgctgtat tgacgccttt ctccagcaga caaatttcgc cgattaatgc
     2221 tccgagtaag gttgctaaaa ccatcgccgg aaggttggcg catttcacca ccagtaaaat
     2281 accaatcccc agcgatgcca aaccaaaaat tgatgtcatg gagacgcgga tacgttccgg
     2341 taagcgttgg ctaagcagtg caccgagcat gccacccagt aatattgcac ttgcgttgat
     2401 aaaagggccg atgaccacaa gagttcctgt taactgtatt gtttgatttt cttattatga
     2461 actttttgcc ttgttgatgg cttgtctggt cgcaaagtcc tggcttgcac gccttagcga
     2521 aaggtgccat gattgcgcga attttctcct ctctgtacgg agtttgcccg atgcacgcca
     2581 tctccttaca ttctctcgct tatcgccgtt tcgcgcgaaa cccttctctt tttatgctac
     2641 tgctcatgcg gtgaagttaa cgcacgctca ctgcaggaca acagtaaaat cagagccttt
     2701 ctgcttttac tgatgtctgg cggtcggagc tggtgaccag tttgacccat atctcatggg
     2761 gcagggtttt acaccttgtc cagtattctt acttccccga aacgggtttg cgcttatgaa
     2821 atcaatgaat attgccgcca gtagtgaact ggtatcccga ctttctactc atcgccgcgt
     2881 ggtggcgttg ggagatactg attttacgga cgtcgcggca gtcgtcatta ccgctgcgga
     2941 tagtcgcagt ggcattctta cgttgcttaa gcgcaccggt tttcatctac cggtgttttt
     3001 gtattccgaa catgctgttg aattacctgc gggcgttacg gcggtaatca acggcaacga
     3061 gcagcagtgg ctggagctgg aatccgcagc ctgtcagtat gaagagaatt tgctgccacc
     3121 gttttatgac acgctgacgc agtacgttga gatgggcaac agcacctttg cttgccctgg
     3181 acatcaacat ggtgcgttct ttaaaaagca tcctgccgga cgccattttt acgatttctt
     3241 tggtgagaac atctttcgcg ccgatatgtg taacgctgac gtaaaattgg gcgatctgct
     3301 tattcatgaa ggatcggcga aagatgcgca gaaattcgca gccaaagtct ttcatgccga
     3361 taaaacctat tttgtgctga acggcacatc ggcagcgaat aaagtggtga cgaatgcgct
     3421 gttaacgcgt ggcgatctgg tgctctttga ccgtaacaac cataagtcga atcatcacgg
     3481 cgcgttgatt caggcggggg cgacgccggt ctatctggaa gcttcacgta acccgtttgg
     3541 tttcattggc ggtattgatg cgcactgttt taatgaagag tatctgcgcc agcaaattcg
     3601 cgacgttgcg ccagaaaaag ccgacctgcc gcgcccgttt cgcctggcga ttattcagct
     3661 gggaacctat gacggcactg tctataacgc ccgtcaggtg atcgataccg ttgggcatct
     3721 gtgtgattac attctgtttg attccgcgtg ggtcggttat gaacaattta tcccgatgat
     3781 ggcggatagc tcgccgctgc tgttagaact taacgaaaac gatccgggga tctttgtgac
     3841 tcagtcggtg cacaaacagc aggcgggatt ctcacagacg tcgcagatcc ataaaaaaga
     3901 taaccatatc cgcggacagg cgcgtttttg cccgcataag cggttgaata acgcctttat
     3961 gctccatgct tctaccagcc ctttctatcc gctgtttgct gcactggatg ttaacgccaa
     4021 aattcatgaa ggggagagtg ggcgtcggct gtgggctgag tgcgttgagt tgggtattga
     4081 gtcgcgcaag gctattcttg cgcgctgtaa gctgttccgc ccgtttatcc cgcccgttgt
     4141 tgatggcaaa ttgtggcagg attatccgac atcagtgtta gccagcgacc gtcgtttttt
     4201 cagttttgag ccgggggcga agtggcacgg ctttgaagga tatgccgcgg atcagtattt
     4261 tgttgatccg tgcaagctgt tactcactac accaggtatc gatgccgaaa ccggcgaata
     4321 tagcgacttt ggcgttccgg cgacgattct ggcgcactat ctgcgtgaga acggcattgt
     4381 gccggagaag tgcgatctca attccattct gttcttatta actccggcgg aaagccacga
     4441 gaagctggca caactggtgg cgatgctggc tcaatttgaa cagcatattg aggatgactc
     4501 gccgctggct gaggtgttgc cgagcgttta taacaaatat ccggtgcgct atcgcgacta
     4561 caccctgcgc cagttgtgtc aggagatgca cgatctgtat gtcagtttcg acgtcaaaga
     4621 cctacaaaaa gcgatgttcc gccagcagag tttcccgtca gtggtgatga acccccagga
     4681 tgcgcatagc gcttatattc gcggtgacgt ggagttggtg cggattcgtg atgccgaagg
     4741 gcgaattgcg gcagaagggg cgttgcctta tccacctggc gtgctttgcg tggtacccgg
     4801 ggaagtctgg ggtggggcgg ttcaacgtta tttccttgca ctggaagaag gggtgaattt
     4861 gttgccggga ttttcgccgg agctgcaagg tgtttatagc gaaaccgatg cggatggcat
     4921 gaaacggttg tacggttatg tgttgaagta agaataaaaa aaacgggtcg ccttctggcg
     4981 acccgttttt ctttgcgtaa ttagtggcta accgtctgtg tacctgtcgg gacacgaacg
     5041 tgtttatatt tgaacatcgc catgaacgcg aaggccagaa ccacggagta accagcgaaa
     5101 atcaaccata cggtctgcca gtcggtaata ccgttttggg tgtacatctc aacaacttta
     5161 ccgctcacga tgccgccgag gatacagccg aagccgttag tcatcatcag gaacatccct
     5221 tgtgcactgg cgcgaattgc cgggctaact tctttttcga caaacaccga accagagatg
     5281 ttgaagaagt cgaacgcaca gccgtaaaca atcatcgata gaaccagcag tacggtgccg
     5341 aacggagtcg ggtcgccgta agcaaacagc gcaaaacgca ggatccacgc cacaatactg
     5401 atcatcatta cgttcttaat accgtagcgg cttaagaaga acgggatggt cagaatgaac
     5461 agggtttcag agatctgcga aatcgacatg atgattgacg catgctgcac aataaagctg
     5521 ctggcaaaca tcggatcttt gtcgaagctg tgcaggaagg tattaccgaa catgttggta
     5581 atctgcagtt ccgcgcccag cagcattgag aagatgaaga agattgccat acgcttgttt
     5641 ttaaacagcg cgaatgcatc gaggcccagc agggttgtcc agctctgatt cgcttgctgt
     5701 ttagcaaccg gaatatgcgg cagagtcagg gtaaacagaa ccagaatggc ggaaagtgct
     5761 gcgccaatat acagctgcat gtggcttaat tcgaagccag acaggctcac cacccacatt
     5821 gccatgataa agccgatggt gccccagata cggattggcg ggaagtcagt aacgatatcc
     5881 atcccggcat tttgcaggcg atagtaagag atggtgttga ttaacccaag cgttggcata
     5941 taagcaaacg agttaatcaa tatcacaagg aacatcgcct ccggcgtcgt gacctgtgcc
     6001 gccatgaaca gcgtgatagc gccaatggtg tggcaaatgg catataccca tttcgcactt
     6061 aaccatttgt cggccacaat ccccagcagc gcaggcataa agaccgcggc gatacccagt
     6121 gagctataaa ctgcgccaat agaagcaccg tcaaacttca gggtaacaaa catataggaa
     6181 ccgagggtcg tcagccaact tccccacaga cagaactgca gaaaagagag gattttcagc
     6241 tgcagcttaa gattcatgtt aatttcctca catcgtgatg cggatgagtg tttgaaaaag
     6301 cacatttgac cgtgcgagat gatgggattc tatcaacttc gcggactggt tttttgttac
     6361 ctgtggcaaa taattgcaaa cattcctaat ttgcaaaacg aaattgtgat atggataaca
     6421 tttttgcccc tgagcatcgt caggggcggt tagtggaatt atcggcggcg gtaggatttt
     6481 tgcgcggtat tcactttata aagataacgg cgagattccg cagagggatg gcgggtcgtc
     6541 agcgtttgat aaacatcgcc cggcgtcatg gtgttaataa tattagccgc ctgaatctta
     6601 tcattcgaaa agactcgcag cacgctgcct gcgccgccgt tataggcggt gatgacggca
     6661 taacgccggg atgtagggtt atcaattccg ccgagataaa cattgttcag catcgccaga
     6721 tacgcggtgc cggtatcaat attgctggca ggatcaaaca agaaactgcg gctcggcgtg
     6781 ccggatttcc cctgcgagcg gaacacatct ttcccggcag tatgttgtac cacctgcatt
     6841 aaccccagcg catcggaacg gctgaccgca tacgggttaa aggaagattc agtctgcata
     6901 attgccagaa tcagcgactc atcaacgcca tatttccgtg acgcctggcg gaccatgccg
     6961 agatatttgt gcgcacgttt atcaaggtgg ttcggcacca tgttaatggt gacgctgtag
     7021 atgatacgca gcccgttgct gcggctcttc agacggtttt tcagcagata atccgcgaag
     7081 ttgcttgcgc gaccttccca gcgaatcggc tgcccggtgt tgtccaccac ctgaccgtaa
     7141 aggaaaggtt ctttcgaaat tgtaatatca tcaacgtcgg aatagagatc gaccgaactc
     7201 ggatcgtcac ccatcagcaa cgttttgata attgcccggc gcaaatgcgc ggcaggttct
     7261 gtcccggcga tggtttcgat agtaatcgta ccgtcatcga agttgatgtg gctgcgggtc
     7321 tgatattgat cggtgtattt cacgtagtcc ttaggaccgg cgatcaccac ctctttgaat
     7381 ccccagatgt tctcaatatt gtgggcaaat tgccccatca gaatatcaaa accgttggtg
     7441 tctttgaccc aggcttcgtt ataggtaccg ccttttttgg tcgtcgaaca ggagatgagc
     7501 aacggcgcaa tcaaagccag cgcgagatat tttttcatca ttccgggtgc gtgttgtgtt
     7561 tgtttttatg caagctgccg gaggcgtgct ccggcactgt ttttattttt tatcttccgg
     7621 cgtatagccc tcgatatgca cctctttacc ctcgaacagg aagttgacca tctcctgctc
     7681 cagcagcttg cggtgctcgg cattcatcat gttgagtttc ttttcattaa tcagcatggt
     7741 ttgtttgtgc tgccactgcg cccaggcttc tttggagatc tcgttataga tgcgttttcc
     7801 cagttcgccg gggtacagct gaaaatcctg accttctgct tcacgttgca ggaaagtaca
     7861 aaaaatcgtt ctgctcataa atcatcctct ttatcgactc acgcgctaaa ccggcgcgcc
     7921 agtgcgtaac tgctgtaaca aacgctccac gggagccgcc aggccaactg acggcggttg
     7981 cgctaagtta taccagagcg cattgccttc atccatgcag ccggtgaatg acgacacggg
     8041 aagccacata ggcacaatat ctaagtggaa atggctgaag gtatgccgaa acgcggtcag
     8101 ctgcgtcagg ttatcggcag caatctgccg ttgcgccagc cactgccgca aactttcttc
     8161 gtcggcaaac tgcgggaaac agtataaacc gccccacaat ccgctcggcg gacgctgcgc
     8221 cagcaatact tcatcttcgt gctgtaacag gagaaagtag ccggtgcgct ccggcagcgt
     8281 ctgtttcggt tttttgcccg gataaagcga ccagctattg ttggtggcgg caatacatcc
     8341 gttttgtagc ggacagagcg aacatttcgg cttcgaacgc gtgcaaatca tcgcgcccaa
     8401 atccatcatc gcctgattaa accgttccac gccgaccgcg ggcgtcacct gctcgcttaa
     8461 actccataat ttattctcga cctctttttt cccaggccag ccgcttacag catagcagcg
     8521 cgccagcacc cgtttgacgt taccgtcgag aatcggaaag tgcttaccca gagaaagcga
     8581 tagaatcgcg cctgcggtgg aacgcccgac gcccggcagt gctgcaactt cctcaaaggt
     8641 ttccgggaat ttaccgccgt gtaaggtcgc cacttgttgt gccgctttat gcagattgcg
     8701 cgcgcgggcg taatagccaa gccccgtcca caagtggaga acttcgtcga gcggcgcatt
     8761 ggcgagatcg gtcaccgtcg ggaagcgcgc cataaagcgt tcaaaatagg ggataacggt
     8821 cgcaacctga gtttgttgca acatcacttc tgagagccat actttgtagg gcgtcttgtc
     8881 aatttgccag ggcagagttt ttcgcccgta tttatcgtac cagtccagaa cctgggctga
     8941 aaattgcgac gcttgcatgg tcaccgaatt cactgttgtt gggggcaaga ttgcagcaca
     9001 gcgacggcag ggtgtaaacc ggaactttcc gcagccacgg ccttatcatg cttgcatccg
     9061 gcaactaact ttggataatg cccgttttca gaacactttc acaagcgact aaatctttat
     9121 gaaaaacgac gtcatttcac cggaatttga tgaaaacggc cgcccactgc gccgtatccg
     9181 tagttttgtg cgccgccagg ggcgactgac caaaggccag gaacatgcgc tggaaaacta
     9241 ctggccggtg atgggcgttg agttcagcga agatatgctg gatttccccg cgctttttgg
     9301 ccgtgaagcg ccggtgacgc ttgagattgg ttttggcatg ggggcgtcgc tggtggcaat
     9361 ggctaaagat cgccccgagc aggacttcct cggcattgaa gtgcattcac cgggcgttgg
     9421 tgcgtgcctg gcttctgcgc atgaagaggg cttaagcaac ctgcgcgtga tgtgtcacga
     9481 tgcggttgaa gtgctgcata aaatgattcc tgacaattca ttgcgcatgg tgcagctctt
     9541 tttccctgac ccgtggcaca aagcgcgcca taataaacgc cgtatcgttc aggtgccgtt
     9601 tgccgaactg gtaaaaagca aactgcagct ggggggcgta ttccatatgg cgaccgactg
     9661 ggaaccttat gcggaacata tgattgaagt gatgtcttct attgacggtt ataaaaacct
     9721 gtcagagagc aatgattacg taccgcgtcc ggcatcacgt ccggtgacga aatttgaaca
     9781 acgtggtcat cgtcttggtc acggagtatg ggacttaatg ttcgagaggg tgaaataatg
     9841 gcaaagaacc gtagccgtcg tctgcgtaaa aaaatgcaca tcgacgaatt ccaggaatta
     9901 ggattttcgg tggcatggcg attcccggaa ggtacatcgg aagaacagat tgataaaacc
     9961 gttgatgatt ttattaacga ggttatcgaa ccgaacaaac tggcctttga cggcagcggt
    10021 tatctggcct gggaaggtct gatctgcatg caggaaatcg gcaaatgcac cgaagaacat
    10081 caggcgattg tgcgtaagtg gctggaagag cgcaacctgg atgaggtacg caccagcgaa
    10141 cttttcgacg tttggtggga ctaagaaagc atacgggcga tgacaaatgc aaaactgcct
    10201 gatgcgctac gcttatcatg cctacaaaga tgcacgatcg agtaggcggg ataaggcgtt
    10261 tacgccgcat ccggcatgga aaacgcgtac tttgttatca atctggggcc agcaaatgct
    10321 ggcctgattt gttcttgagg gaagactatg atgcgcaaaa tgctgctggc ggcagcactt
    10381 tcagtgacgg caatgaccgc tcacgccgac taccagtgca gcgtcacgcc gcgtgacgat
    10441 gtgattgtca gcccgcaaac cgtgcaggtg aagggcgaaa acggcaatct ggtgatcacg
    10501 ccagacggca acgtgatgta taacggtaag caatattccc tgaatgccgc ccagcgcgag
    10561 caggcgaagg attatcaggc tgaactacgt agcacgctgc cgtggattga tgaaggcgcg
    10621 aaaagccgcg tcgagaaagc ccgtattgct ctggataaaa ttatcgttca ggagatgggc
    10681 gaaagcagca aaatgcgcag ccgtctgacc aaacttgatg cgcagctgaa agagcagatg
    10741 aaccgcatta tcgaaacgcg cagcgatggc ctgacgttcc actataaagc cattgatcag
    10801 gttcgcgctg aaggccagca attagtgaat caggcaatgg gcggaatttt acaggacagc
    10861 attaatgaaa tgggcgcgaa agcggtgctg aaaagcggcg gtaacccatt acagaatgtg
    10921 ctgggaagcc tgggggggct gcaatcctca atccaaaccg agtggaaaaa gcaggaaaaa
    10981 gatttccagc agtttggcaa agatgtttgt agccgcgttg tgactctgga agatagccgc
    11041 aaagccctgg tcgggaattt aaaataatcc tctattttaa gacggcataa tactttttta
    11101 tgccgtttaa ttcttcgttt tgttacctgc ctctaacttt gtagatctcc aaaatatatt
    11161 cacgttgtaa attgtttaac gtcaaatttc ccatacagag ctaagggata atgcgtagcg
    11221 ttcacgtaac tggaggaatg aaatggagtt tttcaaaaag acggcacttg ccgcactggt
    11281 tatgggtttt agtggtgcag cattggcatt acccaatatc accattttag caaccggcgg
    11341 gaccattgcc ggtggtggtg actccgcaac caaatctaac tacacagcgg gtaaagttgg
    11401 cgtagaaaat ctggttaatg cggtgccgca actgaaggac attgcgaacg ttaaaggcga
    11461 gcaggtagtg aatatcggct cccaggacat gaacgatgat gtctggctga cactggcgaa
    11521 aaaaattaac accgactgcg ataaaaccga cggcttcgtc attactcacg gtaccgacac
    11581 gatggaagaa accgcttact tcctcgacct gacggtgaaa tgcgacaaac cggtggtgat
    11641 ggtcggcgca atgcgtccgt ccacgtctat gagcgcagac ggtccattca acctgtataa
    11701 cgcggtagtg accgcagctg ataaagcctc cgctaatcgt ggcgtgctgg tggtgatgaa
    11761 cgacaccgta ctggacggtc gcgatgtcac caaaaccaac accaccgacg tagcgacctt
    11821 caagtctgtt aactacggtc cgctgggata cattcacaac ggtaagattg actaccaacg
    11881 taccccggca cgtaagcaca ccagcgacac gccgttcgat gtctctaagc tgaatgaact
    11941 gccgaaagtc ggcattgttt ataactacgc taacgcatcc gatcttccgg ctaaagccct
    12001 ggtagatgcg ggctatgatg gcatcgtgag cgctggtgtg ggtaacggca acctgtataa
    12061 atccgtgttt gacacgctgg cgaccgccgc gaaaaacggt actgcagtcg tgcgttcttc
    12121 ccgcgtaccg acgggcgcta ccactcagga tgccgaagtg gatgatgcga aatacggttt
    12181 tattgcctct ggtacgctga acccgcaaaa agcgcgcgtc ctgctgcaac tggctctgac
    12241 gcaaaccaaa gatccgcagc agatccagca gatcttcaat cagtactaat catttcgccc
    12301 cggtatcgcg ccggggcttt ttcacttcag acttacgtcc attgccaatt ttaattgccc
    12361 taatgataat caccagaata aattattccg cgcgagggtt ttcgggtgaa aaagcaatgg
    12421 attgttggca cggcgctgct tatgttgatg actggtaatg tctgggcaga tggcgaaccg
    12481 ccaactgaaa atatcttaaa agatcaattc aaaaagcagt atcacggcat tctcaagctt
    12541 gatgccatca ccttaaaaaa tcttgatgct aagggtaatc aggccacctg gtcagcggaa
    12601 ggcgatgtct cttccagtga cgatctctat acctgggtcg gtcagttggc agattacgaa
    12661 ctgctcgaac agacctggac gaaagataaa ccggtaaaat tctcggcgat gttaaccagt
    12721 aaaggaacgc ccgcgtctgg ctggacggtg aacttttact cttttcaggc ggcagctagc
    12781 gatcgtggtc gggtggtcga cgatatcaaa acgaataata aatatctgat cgtgaatagc
    12841 gaagatttca attatcgctt tagtcagctt gagactgcgt tgaataccca gaagaactcg
    12901 attcctgcgc tggaaaagga agtgaaagcg cttgataagc aaatggtggc ggcgcagaaa
    12961 gcagcggatg catattgggg aaaagatgca aatggtaagc aaatgacccg cgaagatgcc
    13021 tttaaaaaaa ttcaccaaca gcgtgatgag tttaataaac agaacgatag cgaggcgttc
    13081 gcggttaaat atgacaaaga ggtttatcaa ccggcgattg cggcatgtca taaacagagt
    13141 gaagagtgtt atgaagtgcc gattcagcag aaacgagatt tcgatatcaa cgaacaacgg
    13201 cgacagactt ttctgcaatc acaaaaactt agccgtaaat tgcaggatga ctggataacg
    13261 ctcgaaaaag ggcaatatcc gttaacgatg aaagtttcag aaataaacag taaaaaagtc
    13321 gcgattttga tgaaaattga cgatatcaac caggctaatg aacgctggaa aaaagatacc
    13381 gaacaactgc ggcgcaatgg ggtgattaag taagttttga tatagcgcac gatttgtagc
    13441 aggatgcggc gttgaccccg catccggcaa tcaacgtctg atgcgacgct agcgcgtctt
    13501 atcagaccta caacagctgc cacatgtcgg ctgaggtgct cacgccgcat ccggcaatca
    13561 acgtctgatg cgacgcttgc gcgtcttatc agacctacaa ccgttgccac atgtaggctg
    13621 gataaggcgt tcacgccgca tccggcaata caagtttact cagccagaaa aagctccagc
    13681 agcgaattta aaaacagctt cccatgttcc gtaatctgcc agtaatccgc acattcggtg
    13741 agataaccct gggcaatcgc ctcgtctaac tgtgggcgaa tcacatcttc gcaaagcccg
    13801 gtatacgcac taaactccac gcgcggcgca ggttccagca accggaagcg attcataaag
    13861 aactcaaacg gcttatctgc ggcttcgaca tcacgctggc tttccagata ccttccttgc
    13921 ataaaaccac gcggatgacg cgttttggtg gtacgcagaa tgcgcccatc cgggaaggtc
    13981 actttgccgt gcgcgccgca gccaataccg atgtagtcac caaagcgcca gtagttgaga
    14041 ttgtgctggc actgataacc gggtttggcg taagcggaag tttcatattg ctgataaccc
    14101 gctgcggtta ataactgatg cccctgttcg aaaatatccc acagcgcgtc gtcgtccggc
    14161 agtaccggcg ggcgtgaacc aaacagcgta ttaggttcga tggtcagttg ataccaggaa
    14221 agatgcggcg gattcagttc aatagcctgg cgcagatcgc caagcgcctc ttccagcgat
    14281 tgatccggta gcccatgcat caaatcaagg ttaaagctac gtaaccctaa accgctcgcc
    14341 agctttgccg ctcgtttcgc ttcttgcggg ccatgaatac gtcccagccg tttcagcttt
    14401 tcttcgctaa aactctgtac cccaatagag atacgattta caccggcacg ctgataatca
    14461 acaaagcgat cggcttctac cgtaccgggg ttcgcttcca tggtaatttc tgcatccgct
    14521 gccagcggca aacgcgcacg cacgccgtcc agcagcgttt gcatcgccga gccggaaagc
    14581 aggctcggcg taccaccgcc aataaagatt gtctttactt cacggccctg agcgtaagcc
    14641 acatcgttgt ccagatcgtt aagcagatgc tgaacgtaat cgtcgtgcgg cacttctcct
    14701 ttcaacgcgt gagagttgaa atcgcagtac gggcatttct gcacgcacca cgggatgtga
    14761 atgtagagac tcagcggcgg taatttaacc attacgtaaa gcgtccagca gcagtttcaa
    14821 tgcctgacca cggtgggaaa tggcgctctt ttcttcgcgg gtcagttcgg cagcggtttt
    14881 cccttcggaa ggtacgaaga agattggatc ataaccaaag ccaccagtgc ccgccggttc
    14941 gcgagtaatc acacccggcc agctgccatg gcacaccagc ggggtcggat cttctgcgtg
    15001 acgcagatac accagcacgc agtggaaacg cgcctgacgt tggtcgtccg gtacgtcttt
    15061 cagtgtttcc agcagttttt gcagattctt ttgatcggtc gcgtcttcac cggaataacg
    15121 tgcggagtaa atacccggcg cgccgccaag cacatctacc gccaggccag agtcgtcggc
    15181 aattgccggt aaaccggtca cttttgccgc gtggcgcgct ttcagaatcg cgttttcgat
    15241 aaaggtcagg ccggtttctt cagcggaatc aacgccgagt tctgtttggg ccacgatatc
    15301 aagaccgaag tcgctaagca gcgacgccag ctcacgcact ttaccggcat tgccggttgc
    15361 gaggacaact ttttgcatag gatacctaat taattaacgc cgcgacttct ggcgggattt
    15421 gttgcggatt aatgatttta atttgtttgt ggcggccaag ttcgcctttt tcaatcacca
    15481 cctggctttt ggcaacccgg aattgcttac cgagaaactt caccagatgg ctgttggcct
    15541 gaccgtcaac cggcggcgcg gtaatggcga cttttacctc gtcgccatgt aaaccgacaa
    15601 tagaatcacg gctggctttc ggctgaatat agagccgtaa aaccagaccg tcatcgttaa
    15661 ctgttacggc actcataacg ccatccacag ccccggcagc agcatatttc cggtcgcctg
    15721 taatacttct gcgacaccca tattgatgac atacagcagc agaacgagga tcatcggcga
    15781 gaaatcaatc ccccccattg ccggtagcaa gcggcgaatc gggcgcagca gcggatcggc
    15841 cagctgaatc agcacgtatt caatcgggct acggccctgg cttacccagc tcataatcgc
    15901 catcaccagc aggacccaga aaatcagcag gccgatggtt ttcagcagaa tcagtaaacc
    15961 ggcaatccag atgattggca ggaaggtcac cactttaaac agcacgatgg ctttgataaa
    16021 actgagaata taggcaacca gcagcgaggc gctatcaatg ggtcccattg ccggaataac
    16081 gcggcgcagt ggcccgataa ttggctgcgt taccttcact acaaactgtg agaaggggtt
    16141 gtaaaaatca caatgagccc actgcatcca gatgcgtaat aacagcacca tggtatacag
    16201 ctcaatgacc gttgaaagca ggaaagtcaa cgtattcatg gcgttccttt aattccttat
    16261 tttttagagt aatcacgcgc accaaaaatt gcagtgccga tacgaaccat cgtgctacct
    16321 gccgcaatgg cggcttccat atcgtccgac attcccagag agagcgtgtc gatatgcggg
    16381 tagcgcgttt tcagtccggc aaatgctaca gccatttggc gtgcaacttc aaactgcctt
    16441 acatattctg actcaggcgc agggattgcc atcagcccgc gcagacgtaa acgcggtagt
    16501 tcagcgaccg cagctgccag ctcgtccagt tctgccagtt gaatcccgga cttactgttt
    16561 tcatcactaa tattaatttg aatcagtacg ttaagagggg gaagttctgc cgggcgctga
    16621 tcgttgaggc gggtagcaat gcgcaaacgg tcgatggtat gacaccagtc gaaatgctct
    16681 gccaccaggc ggcttttatt agactgcaac gggccaataa aatgccattc taatcctgtt
    16741 acgcccagtt cctgaaagtg gcgaatttta tctacccctt cctgaacgta gttttcacca
    16801 aattgacgct gcccggcatc aatggcttct gcgatggcgc tcgcaggttt tgttttactg
    16861 actgcaagca gcgtaatttc ttctggagaa cggccgcaac gcgttgcagc cgctgagatt
    16921 ttgtcccgga cctgtgccag gttatgcgca atatcgttca ttttccgagg atcttagtat
    16981 gaatatggaa gaaattgtgg cccttagtgt aaagcataac gtctcggatc tacacctgtg
    17041 cagcgcctgg cccgcacgat ggcgcattcg cgggctaatg gaagctgcgc cgtttgatgc
    17101 gccggacgtc gaagagctac tgcgggagtg gctggatgac gatcagcggg caatattgct
    17161 ggagaatggt cagctggatt ttgccgtgtc gctgacggaa aaccagcgat tgcgtggcag
    17221 cgcattcgcg caacggcatg gcatttcgct ggcgttacgg ctgttacctt cgcactgccc
    17281 gcagctcgaa cagcttggca caccaccgat attgccggag ttactcaaga gcgagaatgg
    17341 cttgattctg gtgacggggg cgacggggag tggcaaatct accacgctgg cggcgatggt
    17401 tggttatctc aatcaacatg ctgatgcgca tattctgacg ctggaagatc ctgtggaata
    17461 tctctatgcc agccagcgat gtttgatcca gcagcgggaa atcggtttgc attgtatgac
    17521 gttcgcatcg ggattgcggg ccgcattgcg ggaagatcct gatgtgattt tgctcggaga
    17581 gctgcgtgac agcgagacaa tccgtctggc gctgacggcg gcagaaaccg ggcatctggt
    17641 gctggcaacc ttacatacac gtggtgccgc gcaggcagtt gagcgacttg tggattcatt
    17701 tccggcgcag gaaaaagacc ccgtgcgtaa tcaactggca ggtagtttac gggcagtgct
    17761 gtcacaaaag ctggaagtgg ataaacagga aggacgcgtg gcgctatttg aattgctgat
    17821 taacacaccc gcggtgggga atttgattcg cgaagggaaa acccaccagt taccgcatgt
    17881 tattcaaact gggcaacagg tggggatgat aacgtttcag cagagttatc agcagcgggt
    17941 gggggaaggg cgtttgtgac gttgccatgc ccgatgcgca agcttatcgg gcctacaaag
    18001 gcaataaacg tctgatgcga cgcttgcgcg tcttatcaga cctacaaccg ctgccacatg
    18061 taggccggat aaggcgttca cgccgcatcc ggcaataagg cgtaaggcgc gatgatatta
    18121 tttacactcc agcaggcgat taaatctcct taatatccct gctcgaaata gctttcgaga
    18181 ataataaccg cagaggcaga gtcgattttg cctttgttga gcgcccgata gccgccctgt
    18241 tcaaacagac cggaacgtgc ctccacggtg ctaagacgct cgtcatggag ctttacttca
    18301 acaccgaaac gaccatgaat acggttagca aatttacgcg ctctggcggt taacggctgt
    18361 tcggtgccgt ccatattcag cggtaaaccg acgatgattt cgtctggctg ccactctttc
    18421 agtaaacgtt cgatgaggtt ccagtccggc gtaccgtcct gtgctttaat tgcaggcaaa
    18481 gggcgagcgg tgccggtaat gcgttggccg accgctacgc caatgctttt ggtgccgaag
    18541 tcgaaggcga gtaaggttcc actcatcagg cgtgtcctgc cacaccaggc atggtgagaa
    18601 tatccacacc aatcagtttt gccgcctcgc gccagcggtc ggcaattggc gttttgaaca
    18661 gaatattcag atctgccggg gcggttagcc acgcgttatc gagaatttct tgttccagtt
    18721 gacctttttc ccaggaggca tagcccagag ccaccaatac gtcagacggt tgtttatcgg
    18781 tgccgagcgt ttccagcaca tcgcgggaag tggtcatcac tgtgttgtct gaaatgcgaa
    18841 tgctggaagc aaaattggag ggcggagtat gcaaaataaa cccgcgatct tcagccagtg
    18901 ggccgccgag cataaccggt ttatccagac ggattgattc atcacgcggc tccggcgtga
    18961 tcttcagctt ttccagaatc ccttcaattt tgagattttc cagcggcttg ttgacgatga
    19021 tccccattgc accattggta ttatgttcgc aaatgtagac cacggaacga cggaaaatcg
    19081 gatcctggag agcaggcatg gcaataagaa agtgatgctg taaattcatt gtcagaggtt
    19141 ctgtttcctg gttcaaaaaa gcaacagcgc ccagtatgcg tggaaagaag ggcgctgtca
    19201 ctcagagtct caacgagatc cttctcgcta aggtgggtta ctactgctgt aaacgtgctt
    19261 cgatggcatc cattaacatt ccggtgatcg acaccggaaa ctctgcttca atctcacgaa
    19321 tacaggttgg gctggtgacg ttaatttcag tcagacggtc gccgatgata tccagaccaa
    19381 caaaaatcag ccctttttct ttcagcgtcg gcccgatctg acgggcgatt ttccagtcac
    19441 tttccgtcag cggacgaggt tcaccgcgac caccggcagc cagattgcca cgggtttcgc
    19501 ccccctgcgg aatacgcgcc aggcagtacg gtactggttc gccgtccacc accagcacgc
    19561 gtttgtcgcc atctttaatg gctggcagat aattttgtgc catgcagtag cgagtgccgt
    19621 gttcggtcaa ggtttcggca atgacgccga ggtttggatc gccttctttc acgcggaaaa
    19681 tcgacgcgcc gcccataccg tccagcggct taagaatgat gtcgctgtgt ttctcccaga
    19741 acgcttttag ctgcgcttta ttgcgcgtaa ccagcgtttc tggcgttaag tcagagaacc
    19801 aggcggtaaa cagtttctcg ttacagtcgc gcaggctctg cggcttgtta acgatcagcg
    19861 tccctttctc ttcggcacgt tccagaatat aggtcgcgta gataaactcg gtatcaaacg
    19921 gcgggtcttt acgcatcagg atcacatcga gatcggccag cggcagatcc tgttcaccga
    19981 cgaatgaaaa ccactcttcg tagttctgtt tcacgttcag cgtgcgggta tgggcgcggg
    20041 cttcgccatt gatcagatac agatcgccca tctccatata gtgaagttcg taaccacgac
    20101 gctgtgcttc cagcaacata gcgaaactgg aatctttctt gatgttgatg tttgcgatgg
    20161 ggtccatcac gatgccgagc ttgatcatta ttcttctccg ttagcccaaa tcgccaaatc
    20221 gtacttgtag cgcggtaatg gcggtgagcg cagttgtctc tgtacgcaaa acgcgaggtc
    20281 ccaacaggat atcagtaaat tgatagcggg cagtcatggc aatttcatct gccgataaac
    20341 cgccttccgg gccaatcagc aggcggacgc gttcaaccgg taaaggcaac gtattgatgc
    20401 tgttactggc gcgcgggtga agattcagtt tcagtccttc atcctgctct gcacaccagg
    20461 cttccagatc catcgctgga cggatttccg gcacccggtt acgaccacac tgctcacagg
    20521 cagcaattgc aatcttctgc cactgctgga gcttcttgtt cagacgttca ctatccagtt
    20581 taacgccgca gcgctcagaa aaaagtggcg taatgaggct tacaccgagt tcgatcgatt
    20641 tctggatcgt aaattccatt ttttcaccac gcgacatcac ctgaccgagg tgaatatgca
    20701 gcggagattc gcgatcgtcg atctggcctt ccagcacctt cacttccacg ctttttttgc
    20761 tggcgctggt aatttcggcg tcaaagacct ggttgctgcc gtcaaacaat tgcaacgcct
    20821 gtcccggccc catacgcagt acgcgcccaa tatggttggc ggcatcttcg caaagcgcga
    20881 tgtgagaatg gctggtcagt ggttcaggat gataaatgcg ggggatacgc atagttaaaa
    20941 atccgcgtcg tctccccacg cggttgtacg cgtggggtag gggttaacaa aaagaatccc
    21001 gctagtgtag gttagctctt tcgcgcctgg caagcgcgtt gcacatacgg gttatgattg
    21061 ccctgtacct tcgcgatgcg ttcatcgcgc tcgcactccc agtcggtaac cggatacatc
    21121 ttgtcccatg cgttgaacag ctgcgtttgc tggcgagaga gtgtcaggtt gtaatggtca
    21181 cgcatataga agtaggtgcg cgcaatggca ccgcgtgcac gcgctggtgg ttcggcagct
    21241 ttttctttga aatcgacctt catggcgcat tgaccgtact ggccttcacc gccattccac
    21301 tggctgtaca taaagttgcc gcgatcgcca ttcacctcgc cgactgacgg ttgcaggtta
    21361 tgcatatcgc tttccatctt gcgatagacc ggatctttgg cgcaattttt acgtccaccg
    21421 tcctgccagc actggcgctg gtgaccgaat tgccaggcgg gaacgacgtg ttcccactct
    21481 acgcggctgg cgcggttttc atttttgcgc acctgatagc cgcacgattg cagatcgaca
    21541 acgccttttt tgccctgcca gttaatttta catccgcaat aaaacgtacc gggcgcgtca
    21601 gcgtggactt ttaccgccgc ggctttcgcc tgagaaaaac tattgatacc ttcggccaac
    21661 gccgggccgg aaaatgctgc gctcagtacc accgcagcaa tagacaaata acggtacatc
    21721 acttactccg tgttaaaacg agccagcaac gtagcgaaag ttgttttggt atgcaatcag
    21781 ccagatcagg aaagttcctg atagttcaga tggttatttt gcgaccagtt gttcgccgca
    21841 gtgaacacag cgatagacgg cttcgccacg aactacgcga ttatgacggc gtacggtaag
    21901 ctgatgttcc tgacacttac agctgtaggg gaaggtgttg cggcgcacgg attgcagttc
    21961 gaactgatgc gtacggcggg ctggaacgcc cagcacactt tccatcatcc acttccactc
    22021 tttgccatgt ggtgctacgc ggccaaaatg tttccatacc agcaaatgtg ccagttcgtg
    22081 cggtaccact tcttcaataa aggcttcact gttttccaac agcaaaacgg gattaaggcg
    22141 aatttcatag ctttccagcc aggctgttcc ggcagaggtt ccgcgctggg tgtaagagag
    22201 ttttggctcc gggtagttac gccccaattt caggttggcc tgggcgagtt tttcccgcag
    22261 gcgacgcata acggcctgct ggatggcgat agggagacgg gatgttttca tagcggcaga
    22321 ggatagagcg aagaaaagat gactgcaaga ggtggcttcc tccacgacgg gaggaagcct
    22381 ggggagatta atcgtgagcg ccgatttcgc gcagtttacg acctttcatc agattacgtt
    22441 caatatgttc cagcgaaacg tgtttggttt ctggcaccag ccacagtgtc agcaggataa
    22501 acaggacgtt cagacccgcg taaacccaga aggtattagc gttgcccagc gtgttgagca
    22561 tggtcaggaa cgttgcgcca acgatcatgt tggcaatcca gttggtggcg gtggagcagg
    22621 tgatgccaaa atcgcggcct ttcagcggct gaatttcgga gcacagtacc caaatcagcg
    22681 gaccggcact catggcaaaa ccgacaataa acatcagcag catggcgatg gcgaaatact
    22741 gcgccgacgg agagtggata ccgatatgca tcattgtacc gagtacgccc ataccagcag
    22801 ccatcaccag gaagcccagc gttagcgttg gtttacgtcc ccagcggtca acaaggccga
    22861 ttgcgataaa ggtggcaagt acgttggtca ggccgacaat cactgtcccc cacatttgct
    22921 cggtggtgtt ggtataaccc gccagttcga agattttcgg cgcgtaatac atgatgacgt
    22981 tcatcccggt gaattgctgc attacctgta acagtacgcc aaggaacacc gcgcggcgga
    23041 agttgctatt ctctttaaac agcgcccagc cactctgttt aacctgcaaa ctttcacgga
    23101 tttcatccag ttcgcgtttc gcttccgcgc tggtgtcacg caggcgtagc agcacgcgtt
    23161 cggcatcaac aaaacggcgt ttggcggcaa accaacgtgg gctgtctggc aggaagaaga
    23221 caccaatcag cagcaaaatt gccgggatga taatcacacc cagcatccag cgccatgcac
    23281 cggtgtagct gaaggcggta tcagaaagat aagcaccgag gatcccgatt gtgatcatta
    23341 actgatacat cgagatcata ctgccgcgaa ttttttccgg cgcaatttca gagaggtaca
    23401 gtggtgcggt ataagaggcc acacctaccg ccagccccag cagaacgcgg gaaagaatca
    23461 gtacttcaac gtttggcgca gccgcagaga acagcgaacc ggcaacaaac aaaattgcgc
    23521 cgatcatcag gctctttttg cgcccgagtt taaaggagag ccagccgctg cccaccgcac
    23581 cgactgccgc accgaacatc atggagctta cgacccattc ttgcgtgtgc gaagtaatct
    23641 ggaattcatc tgcaataaac ggcagtgcgc cagcaattac accgatatcc aggccaaaga
    23701 gtaatcccgc cagagcggca aggaagcaga cgaaaaacgt cattgccttg tttgactgcc
    23761 cctgtttttt agcgtcaggc atgatgccct ccaatatggt tattttttat tgtgaattaa
    23821 gataggtgag tacgacgtaa aaagatgtga agcaaatcac atcagtgtaa tcgcttacac
    23881 taatatttat cttagttatg atttaagtgc atgaaaataa ttgagttata tcatcataga
    23941 ttaaacgctg ttatctgcaa ttaagacttt actgaaaaga aatgtaacaa ctgtgaaaac
    24001 cgaataataa tatggaaata ttacaaatct ttgtctgttg tggttggtgt aatcggtttc
    24061 agttgtgatg ataatttgcg gtgaaacgtg atcaggaaga atgatgttat tgtgcaggcg
    24121 gtttttttgc cggatgcggc gtgaacgcct atccaccgcg gtgaggtaaa agaaaaaggc
    24181 cagcgcgagg ctggcctttg aaagcaggtg aagaaagatt acttcagacc ggcagcatcg
    24241 cgcagcagct gcgctttgtc ggttttttcc cacgggaaat gttcacgacc aaagtgaccg
    24301 tatgctgcgg tttctttgta gatcgggtgc agcagatcca gcatctgaat cagaccgtat
    24361 gggcgcaggt cgaagaactc acgtaccagc agagtcagtt gttcagaagg cactttctca
    24421 gtaccgaaag tttctaccat gatggaagtc ggttcagcca cgccgattgc gtaggaaacc
    24481 tgaatttcac aacgatcggc caggccagca gcaacgatgt ttttcgcgac ataacgtgct
    24541 gcgtaggctg cggaacggtc cacttttgat ggatctttac cagagaatgc accgccaccg
    24601 tgacgcgcca tgccgccgta ggtatcaacg ataattttac gaccagtcag accgcagtca
    24661 cccatcgggc caccgataac aaaacgaccg gtcgggttga tgaagaattt ggtggcagaa
    24721 gtcagccatt cagcgggcag aatcggcttg atgatctctt ccattaccgc ttcttgcagc
    24781 gatttctggt cgatctcttc agagtgctga gtggaaagca cgacagcatc gataccaacg
    24841 attttgccgt cgtcatactg gaaagtcacc tggcttttcg cgtccgggcg cagccacggc
    24901 agagtgccgt ttttacgcac ttcagcctga cgctgtacca gacggtgtgc ataggtgata
    24961 ggtgctggca tcagcacgtc ggtttcatta gttgcgtagc caaacatcag accctggtca
    25021 cccgcgccct gttccagcgg atcggcacgg tcaacgccct ggttgatgtc aggagactgt
    25081 ttgccgatag cgctcagaac tgcacaggag ttagcgtcaa agcccatgtc ggaatgcaca
    25141 tagccaattt cgcgaacggt gttacgggtg atctcttcga tgtctaccca ggcgctggtg
    25201 gtgatttcgc cgccaactaa aaccatgccg gtttttacgt aggtttcgca agcaacgcgt
    25261 gctttcggat cctgttcgag gatcgcgtct aaaacggcat cagagatttg gtcagcaatt
    25321 ttgtcaggat gcccttcaga gacggactcg gacgtaaaaa ggtgttttgc catatttaat
    25381 atcacctaaa gagaatttgg ttagctcaaa ctgttgtgtg gattttctgt ggtagcggat
    25441 cctaccacga ctctgcaggt taaaaacact ggcagtctga gtgttaatcg gtatggatgg
    25501 attaacatct ggatggctat tttaggtcaa ttcttcaccc gatttccact tttttttgaa
    25561 tcgtgtctca ttctgttaaa aacgtggctg gaaatttttc ctgacaatgc cggcattctg
    25621 cgtatttatc ttttgcaatt ttctgccatt gtggggtata aaacgcggcg cgcggcttaa
    25681 ataaaaagca cacgacgttt ctttcgtgtt gccacttcca gccgggttga aatcagagtt
    25741 ttggcttgtg ggttcgtctt aacaggcggc cgtggaggtg atacgaaata atgaaccgtt
    25801 gtctgctgct taacctgtct caccgttctg gtgaagattc gttccccgca ctctgcatct
    25861 ctgctttgca tacctgccga tgttataccc atctcggcgc ttctcaggat tcaagagctg
    25921 gttacagtta ctgaggactg aacaagggcg ctcttgtaaa aacaagagtt ttctcgtggt
    25981 ttcgccgaac tgtcacactt acgttcggtt atgtgcttaa taatgttatg aaaaagaaac
    26041 cggttgcgca gttggagcgt cagcattcac tgctggaaaa tccatgtgct tatgggttgt
    26101 tatcgcagtt ccaggctgcg atagtcgtta actgttttac acttaataaa ataatttgag
    26161 gttcgctatg tctgacgaca tgtctatggg tttgccttcg tcagcgggcg aacacggtgt
    26221 actacgctcc atgcaggagg ttgcaatgag ctcccaggaa gccagcaaga tgctgcgtac
    26281 ttacaatatt gcctggtggg gcaataacta ctatgacgtt aacgagctgg gccacatcag
    26341 cgtgtgcccg gacccggacg tcccggaagc tcgcgtcgat ctcgcgcagt tagtgaaaac
    26401 tcgtgaagca cagggtcagc gtctgcctgc actgttctgt ttcccacaga tcctgcagca
    26461 ccgtttgcgt tccattaacg ccgcgttcaa acgtgcgcgg gaatcctacg gctataacgg
    26521 cgattacttc cttgtttatc cgatcaaagt taaccagcac cgtcgcgtga ttgagtccct
    26581 gattcattcg ggcgaaccgc tgggtctgga agccggttcc aaagccgagt tgatggcagt
    26641 gctggcacat gctggcatga cccgtagcgt catcgtctgc aatggttata aagaccgcga
    26701 atatatccgc ctggcattaa ttggcgagaa gatggggcac aaggtctatc tggtcattga
    26761 gaagatgtca gaaatcgcca ttgtgctgga tgaagcagaa cgtctgaatg tcgttcctcg
    26821 tctgggcgtg cgtgcacgtc tggcttcgca gggctccggt aaatggcagt cctccggcgg
    26881 ggaaaaatcg aagttcggcc tggcggcgac tcaggtactg caactggttg aaaccctacg
    26941 tgaagccggg cgtctcgaca gcctgcaact actgcacttc cacctcggtt cgcagatggc
    27001 gaatattcgc gatatcgcga caggcgttcg tgaatccgcg cgtttctatg ttgagctgca
    27061 caagctgggc gtcaatattc agtgcttcga cgtcggcggc ggtctgggcg tggattatga
    27121 aggtactcgt tcgcagtccg actgttcggt gaactacggc ctcaatgaat atgccaacaa
    27181 catcatctgg gcgattggtg atgcgtgtga agaaaacggt ctgccgcatc cgacggtaat
    27241 caccgaatcg ggtcgtgcag tgactgcgca tcacaccgtg ctggtgtcta atatcatcgg
    27301 cgtggaacgt aacgaataca cggtgccgac cgcgcctgca gaagatgcgc cgcgcgcgct
    27361 gcaaagcatg tgggaaacct ggcaggagat gcacgaaccg ggaactcgcc gttctctgcg
    27421 tgaatggtta cacgacagtc agatggatct gcacgacatt catatcggct actcttccgg
    27481 cacctttagc ctgcaagaac gtgcatgggc tgagcagctt tatttgagca tgtgccatga
    27541 agtgcaaaag cagctggatc cgcaaaaccg tgctcatcgt ccgattatcg acgagctgca
    27601 ggaacgtatg gcggacaaaa tgtacgtcaa cttctcgctg ttccagtcga tgccggatgc
    27661 gtgggggatc gaccagttgt tcccggttct gccgctggaa gggctggatc aagtgccgga
    27721 acgccgcgct gtgctgctgg atattacctg tgactctgac ggtgctatcg accactatat
    27781 cgatggtgat ggtattgcca cgacaatgcc tatgccggag tacgatccag agaatccgcc
    27841 gatgctcggt ttctttatgg tcggcgcata tcaggagatc ctcggcaaca tgcacaacct
    27901 gttcggtgat accgaagcgg ttgacgtgtt cgtcttccct gacggtagtg tagaagtgga
    27961 actgtctgac gaaggcgata ccgtggcgga catgctgcaa tatgtacagc tcgatccgaa
    28021 aacgctgtta actcagttcc gtgatcaagt gaagaaaacc gatcttgatg ctgaactgca
    28081 acaacagttc cttgaagagt tcgaggcagg tttgtacggt tatacctatc ttgaagatga
    28141 ataattaaga ttattctgct ggcgggtcga tttatttacg taaatcggtc cgctttcttt
    28201 ttatatattc ttaaaaataa tttagctgct tttcttatta aataatctcc ctacaatcgc
    28261 tgcattaatt aaatgcagag ggaacggtta tgaaaaaatg gaaagtacgc agcgcgttgg
    28321 tggcattaat cgtgctgctg gccggttgtt ccagtaatgc gcagtataat agcagtgcat
    28381 cgggcaatgt cggcaccgcc tggggtgggg atgtgcactc aacggttcag ggcgtatctg
    28441 ctgagcgagc ctggcgcgat ccggcggaga tgatcgttat tagttattcg actaatgttc
    28501 cttccggcta cgatcgcgtt tatagcattc gtattaacga actggaatat gctattcgcg
    28561 atggtaattt taattctcta cctattacgc gtgtttatga ttcatctaat aatgaaccac
    28621 gctatattgt ccatgccaga gtcggaatga attatcaatt atatgtccgt aattacagtc
    28681 gtaacaccaa ctacgagatc gttgctacgg ttgatggcct ggatgtgctg aacggcaagc
    28741 aaggttcgct gaataacaac ggctatattg tgaatgccgg agattcgtta gcgattaaag
    28801 gattccgcaa agataaacat accgaagcgg cttttcagtt cgccaacgtt gctgattcct
    28861 acgcagccaa ttcggcgcaa ggcgatgtgc gtaataccgg tgtaatcggc tttgccgcct
    28921 ttgaattaca aggccaggcg caaaacgcgc tgccaccgtg ctctggacaa gcctttccgg
    28981 cagataacaa tggctatgcg ccgccaccgt gtcggaaata gcaacctcgc ttgatgcctc
    29041 gcccatttaa ggcgataatt agcacaaact tgtgtttcgc aaatccaacc cttcctcgtc
    29101 gggcctaacg acgcggaagg gtttttttat atcgactttg taataggagt ccatccatga
    29161 gcaccttagg tcatcaatac gataactcac tggtttccaa cgcctttggt tttttgcgcc
    29221 tgccgatgaa cttccagccg tatgacagcg atgctgactg ggtgattact ggtgtgccgt
    29281 tcgatatggc cacttctggt cgtgcggggg gacgccacgg tccggcagct atccgtcagg
    29341 tttcgacgaa tctggcctgg gaacacaacc gcttcccgtg gaatttcgac atgcgtgagc
    29401 gtctgaacgt cgtggactgt ggtgatctgg tatatgcttt cggcgatgcc cgtgagatga
    29461 gcgagaagct gcaggcgcac gccgagaagc tgctggctgc cggtaagcgt atgctctcct
    29521 tcggtggtga ccactttgtt acgctgccgc tgctgcgtgc tcatgcgaag catttcggca
    29581 aaatggcgct ggtacacttt gacgcccaca ccgataccta cgcgaacggt tgtgaatttg
    29641 accacggcac catgttctat accgcgccga aagaaggtct gatcgacccg aatcattccg
    29701 tgcagattgg tattcgtacc gaatttgata aagacaacgg ctttaccgtg ctggacgcct
    29761 gccaggtgaa cgatcgcagc gtggatgacg ttatcgccca ggtgaaacaa attgtgggtg
    29821 atatgccggt ttacctgacc tttgatatcg actgcctgga tcctgcattt gcgccgggaa
    29881 ccggtacgcc ggtaattggc ggtctgacct ccgatcgcgc tattaaactg gtacgcggcc
    29941 tgaaagatct caacatcgtt gggatggacg tagtggaagt ggctccggca tacgatcagt
    30001 cggaaatcac cgctctggca gcggcaacgc tggcgctgga aatgctgtat attcaggcgg
    30061 cgaaaaaggg cgagtaagca ccagatgcga tgcgcacggg taaaacgtgc cattaatgtc
    30121 ggatgcggcg tgaacgcctt atccgaccta cgttcggcac ccgtaggccg gataagatgc
    30181 gccagcatcg catccggcaa tgcgcacaag gtaacaaatg tgccattcat gtcagatgcg
    30241 gcgtgaacgc cttatctgac ctacgttcga caccaccagg ctttacttaa tcccatccgc
    30301 gctcatgcga tcgcgaatat gctgggcgcg ttcggcggat gcaggatggt cgtcaaacat
    30361 tgagctttgg cgaccttctt ccagttttgc cagtttttca aagctggtgg caagacctgc
    30421 cgggctgatg ccgcgttggc gcagaagatc gtaagaataa tcatcggctt ctgcttcctg
    30481 gcgctgggag aattgcgaat taactaattt ctcgcccaga tcaccgagtt gtgattgaga
    30541 taaactgccg acaatcccgc ccgccgaggc cgcagctact cgtacggcat ttgtaccaag
    30601 tgccacctgc attccttttt tcacatggcc taatgccacg tgccccattt cgtgaccgat
    30661 caccgcttcg acttcgttat ccgtcatcat atccatcagc ccgctataga cacggataca
    30721 gccgttagcc attgcaaagg cgttcacatc cttcgccata tacactttgt aatttaccgg
    30781 ctgaccgttg atattgttgc ctagcgcatt ggcaatagtt gtcagacgtt tagcgtattc
    30841 gctattggct ggcgcaatcg tcgccttgct gtccatctcc tgacatgcct gatcgctcag
    30901 ggttttcacc tgcgcatcac tcaaactgta agcctgaaaa gcttccgctc ctgatgagag
    30961 cagtccgttg gagtccatat tctggcaacc ggtcagtacc gttgccacgc tcattgctac
    31021 caataaggcg cgaattttca tgttttttct tccgcacaag gtcaggatta ttctaaaaat
    31081 gcgccgtttg caggtgaacc gacgctcagt ctcagtataa ggaaaagcgc agcggacggg
    31141 cgagtagatt gcgcaacatg cgagcatgat ccagagattt ctgaagcggc aaaaggatgt
    31201 tccatgtaca tgacgcgcgg cttgcggtaa attgttggca aattttccgg cgtagcccaa
    31261 aacgcgctgt cgtcaagtcg ttaagggcgt gcccttcatc atccgatctg gagtcaaaat
    31321 gtcctcacgt aaagagcttg ccaatgctat tcgtgcgctg agcatggacg cagtacagaa
    31381 agccaaatcc ggtcacccgg gtgcccctat gggtatggct gacattgccg aagtcctgtg
    31441 gcgtgatttc ctgaaacata acccgcagaa tccgtcctgg gctgaccgtg accgcttcgt
    31501 gctgtccaac ggccacggct ccatgctgat ctacagcctg ctgcacctca ccggttacga
    31561 tctgccgatg gaagaactga aaaacttccg tcagctgcac tctaaaactc cgggccaccc
    31621 ggaagtgggt tacaccgctg gtgtggaaac caccaccggt ccgctggggc agggtattgc
    31681 caacgcagta ggtatggcga ttgcagaaaa aacgctggcg gcgcagttta accgtccggg
    31741 ccacgacatt gtcgaccact acacctacgc cttcatgggc gacggctgca tgatggaagg
    31801 catctcccac gaagtttgct ctctggcggg tacgctgaag ctgggtaaac tgattgcgtt
    31861 ctacgatgac aacggtattt ctatcgatgg tcacgttgaa ggctggttca ccgacgacac
    31921 cgcaatgcgt ttcgaagctt acggctggca cgttattcgc gacatcgacg gtcatgacgc
    31981 agcatccatc aaacgcgcag tagaagaagc gcgcgcagtg actgacaaac cgtccctgct
    32041 gatgtgcaaa accatcatcg gtttcggttc cccgaacaaa gccggtactc acgactccca
    32101 cggtgcgccg ctgggtgatg cagaaatcgc tctgacccgc gaacagctgg gctggaaata
    32161 cgcaccgttc gaaatcccgt ctgaaatcta tgcgcagtgg gatgcgaaag aagcaggcca
    32221 ggcgaaagaa tctgcatgga atgagaagtt tgcggcttac gcgaaagctt atccgcagga
    32281 agcggctgaa tttacccgcc gtatgaaagg cgaaatgccg tctgacttcg acgccaaagc
    32341 gaaagagttt atcgctaaac tgcaggctaa tccggcgaaa atcgccagcc gtaaagcgtc
    32401 tcagaatgct atcgaagcgt tcggcccgct gttgccggaa ttcctcggcg gctccgctga
    32461 cctggcaccg tctaacctga ccctgtggtc tggttctaaa gcaatcaacg aagatgctgc
    32521 gggtaactac atccactacg gtgttcgcga gttcggtatg accgcgattg ctaacggtat
    32581 ctccctgcac ggtggcttcc tgccgtacac ctccaccttc ctgatgtttg tcgaatacgc
    32641 acgtaacgcc gtacgtatgg ctgcgctgat gaaacagcgt caggttatgg tttacaccca
    32701 cgactccatc ggtctgggcg aagatggccc gactcaccag ccggttgagc aggttgcttc
    32761 tctgcgcgtg accccgaaca tgtctacatg gcgtccgtgt gaccaggttg aatccgcggt
    32821 cgcgtggaaa tacggtgttg agcgtcagga cggtccgacc gcgcttatcc tctcccgtca
    32881 gaacctggcg cagcaggaac gtactgaaga gcaactggca aacatcgcgc gcggtggtta
    32941 tgtgctgaaa gactgtgctg gtcagccgga actgatcttc atcgcgaccg gttcggaagt
    33001 tgaactggct gttgccgcct acgaaaaact gactgccgaa ggcgtgaagg cgcgcgtggt
    33061 ttccatgccg tctaccgatg cattcgacaa gcaggatgcg gcttaccgtg aatccgtact
    33121 gccgaaagcg gttactgcac gcgttgcggt agaagcgggt attgctgact actggtacaa
    33181 gtatgttggc ctgaatggcg ctatcatcgg tatgaccacc ttcggtgagt cagctccggc
    33241 agagttgctg ttcgaagagt tcggcttcac cgtcgacaac gtcgttgcga aagcaaaaga
    33301 actgctgtaa ctagcatttc gggtaaaaag gtcgcttcgg cgaccttttt tattaccttg
    33361 atatgtccgt ttgcggacaa gcaatagata aagcgtgttg tagatcgcaa atatttatat
    33421 gcaataaata tcaattatgt aatatgcatc acgatatgcg tgttgacatt tgttgttata
    33481 tctataactc aatgttatat aagaaattaa taattcactg ttttcaaaac accggtttcc
    33541 ctgctcaatt gctttcatta aaccgcacga gtattttatt tttaccgccg gacggtaccc
    33601 taaaaataaa ataaggataa tttatgcggc ttagtgacta ttttccagaa tcatcaatct
    33661 cagttataca ttcagcaaaa gattggcagg aagctatcga tttctcgatg gtatcattgc
    33721 tggataaaaa ctatatcagc gagaattaca ttcaggcaat taaagattcc accattaaca
    33781 atggccctta ttatattctc gcaccaggcg tggcaatgcc tcatgcgcga ccggaatgtg
    33841 gggcgcttaa aaccgggatg tctttgacat tacttgaaca aggtgtttat tttccgggga
    33901 atgacgaacc aattaaatta ctcatcggac tctctgctgc cgatgccgat tcgcacattg
    33961 gcgctattca ggcgttaagt gaattactgt gcgaagaaga aatactcgaa caactcttaa
    34021 cagcatcatc agaaaaacaa ttagcggaca ttatcagccg cggataatta ctctctcctc
    34081 tacaggataa tattatggaa aacaagtctg ctcgtgcaaa ggtccaggct tttgggggct
    34141 ttttgactgc aatggtcatc cccaatattg gtgcttttat tgcctggggt tttattactg
    34201 cgttatttat tcccaccggt tggctgccta acgaacattt cgccaaaatt gtcggcccga
    34261 tgattaccta tttattgccc gtgatgattg gttctaccgg tggtcatctg gtcggcggta
    34321 aacgcggcgc ggtcatgggc ggaataggta ctattggtgt gatcgtcggc gcagagatcc
    34381 cgatgttcct tggctcaatg attatggggc cgctcggtgg tctggtcata aaatatgttg
    34441 ataaggcact ggaaaaacgc atacctgccg gttttgagat ggttatcaat aacttctcat
    34501 taggtatcgc ggggatgctc ctttgcctgc tgggttttga agtgatcggc ccggcggtgt
    34561 taattgccaa tactttcgtc aaagagtgta ttgaggcgct ggtacatgcg ggttatctgc
    34621 ctctgctgtc agtcatcaat gaaccggcga aagtgctttt ccttaataat gcgatcgatc
    34681 agggcgtcta ttatccgctg ggaatgcaac aggcttcggt taacggtaaa tccatcttct
    34741 ttatggtggc ctctaaccca ggtccgggcc tggggctgct gctggcgttt accttgtttg
    34801 gtaaagggat gagtaaacgt tctgcgcccg gggcgatgat tattcacttc ctcggtggga
    34861 tccacgaact gtatttccca tatgtgctga tgaagccgct gactattatt gccatgattg
    34921 cgggcggtat gtctggcacc tggatgttta acttactgga cggtggtctg gtggctggcc
    34981 caagtccggg gtctatcttt gcttacctgg cgctgacgcc gaaaggttcg ttcctggcga
    35041 caattgccgg tgttacggta ggtaccctgg tgtcctttgc tatcacttcg ctgatactga
    35101 agatggaaaa aacggtggaa acggagagcg aagatgagtt tgctcagtca gccaatgcgg
    35161 ttaaggcgat gaaacaagag ggtgcattct cgttaagcag ggttaagcgt atcgcctttg
    35221 tttgcgatgc ggggatgggc tccagtgcga tgggcgcgac caccttccgt aaacgcctgg
    35281 aaaaagcggg gctggcaatt gaagtaaaac attacgccat agaaaacgtg cctgcggatg
    35341 cggatatcgt cgttactcat gccagtctgg aagggcgcgt gaaacgtgtg acggataaac
    35401 cactgatatt gattaataac tatattggcg atccaaaact cgacacttta tttaatcaat
    35461 taaccgccga acataaacac tgattacaga ggtaaaaatg aaaaccaaag ttgctgctat
    35521 ttatggcaag cgggatgtcc gtctgcgcga atttgaactg ccagaaatta ccgataatga
    35581 attactggtg agtgtgattt ctgacagcgt ctgtttatcc acctggaaag cggcgttact
    35641 cggtagtgaa cataaacgcg tacccgacga tttagaaaat catccggtca ttaccgggca
    35701 tgaatgtgcc ggggttattg tcgaagtggg taaaaatctc actggcaaat ataaaaaagg
    35761 ccagcgtttt gtattgcaac cggcgatggg gttaccaagc ggatattcag cgggctacag
    35821 ctacgaatat tttggcggca atgccactta tatgattatt cccgaaatcg ccattaattt
    35881 gggctgcgta ttaccgtatc acggctctta ttttgctgcg gcgtcgctgg cagagcctat
    35941 gtgctgcatt attggcgctt atcatgccaa ttatcacacc acgcaatatg tttatgagca
    36001 tcgcatgggc gtcaaacctg gcggcaatat tgcactgctg gcgtgtgcag gtccgatggg
    36061 cattggcgct atcgattacg ccattaacgg cggcatacaa ccgtcgcggg tggtggtggt
    36121 cgatatcgac gacaaacgtc tggcgcaggt acagaagctg ctgccggtgg aactggcggc
    36181 cagtaaaggc attgagctgg tgtatgtgaa taccaaaggg atgagcgatc ctgtccagat
    36241 gctgcgggcg ctgacaggag atgccgggtt cgatgacatt tttgtttatg cggcggtgtc
    36301 tgctgtcgtt gagatggctg atgaattact ggcggaagat ggctgtctga acttctttgc
    36361 cgggccgacg gataaaaact tcaaagtgcc gtttaatttc tacaacgttc attacaacag
    36421 cacgcacgtc gtcggtacat ccggcggttc aacggacgac atgaaagagg cgattgccct
    36481 tagcgccaca gggcagttac agccctcgtt tatggtgacc catatcggtg gcctggatgc
    36541 ggtgccagaa accgtgctca atctgccgga tatccctggc ggtaaaaaac tcatttataa
    36601 cggcgtgacc atgccgctca ctgccattgc cgattttgcc gaaaaaggca aaaccgatcc
    36661 gctgtttaaa gagttggcgc ggctggttga ggaaacgcac ggcatctgga atgaacaggc
    36721 cgagaaatat ctgctggcac aatttggcgt tgatatcggg gaggccgcgc aatgatgtcc
    36781 ctggcgtggc cattatttcg cgttacggaa caggcagcgt tggctgcctg gccgcaaacc
    36841 ggatgtggcg acaaaaataa aattgatggc ctggcggtca ccgcgatgcg ccaggcatta
    36901 aacgacgtcg cttttcgtgg acgagtggtt atcggcgaag gagaaattga ccatgcgccg
    36961 atgctgtgga ttggcgaaga ggtgggcaaa ggcgatgggc cagaagtcga tatcgcggtt
    37021 gatcccattg aaggcacgcg gatggtggcg atggggcaga gcaatgcgct ggcggtcatg
    37081 gctttcgccc ctcgagatag cctgttacat gcgcccgata tgtatatgaa aaagctggtt
    37141 gttaatcgac tggctgccgg ggcgattgat ctgtctttgc ctctggcgga caacttgcgt
    37201 gacgtggcgc gggcgttagg taaaccgctg gacaagctgc gcatggttac gctggataaa
    37261 ccgcgtctga gtgccgcgat tgaagaagca acacagctgg gcgtgaaggt ttttgccctg
    37321 ccggatggcg atgtcgccgc cagcgtgctg acctgctggc aggataatcc ttacgatgtg
    37381 atgtacacca tcggcggtgc gccggaaggc gtgatttctg cctgtgccgt taaagcatta
    37441 ggcgggaata tgcaggccga gctgattgac ttttgtcagg ccaaagggga ttacacggaa
    37501 aatcggcaga ttgcagagca ggagcgcaag cgttgcaagg caatgggtgt tgacgttaac
    37561 cgtgtgtact cgctcgatgt actggtaagg gggaacgata tcctctttag cgctacgggc
    37621 gtcacgggcg gcgagttggt gaacggtatc caacagacgg cgaatggagt gcggacgcag
    37681 acattactga tcggcggcgc ggaccaaacg tgtaatataa tagactccct gtattgatgg
    37741 tgatctatcg gaagaccaat ggcgacgctg tcagaagatg atgtgcttga gcaactggat
    37801 gcacaggaca atttattttc atttatgaaa actgcgcatt ctattttgct ccaggggata
    37861 cgccagtttc tgccgtcgct gtttgtcgat aacgatgaag agagcgtcga atatgcagtg
    37921 aagccgttac tcgcccaaag cggcccgctt gacgatattg atgttgcact gcgtttgatt
    37981 tatgcgctgg ggaaaatgga taaatggctg tacgccgata tcacgcattt ttcccagtac
    38041 tggcattacc tgaaagaaca ggatgaaacg ctcggatttg ccgatgacat gacttgggat
    38101 tttatcagca acgtcaatag cataacctgc aatgcgacgc tctatgacgc gttgaaagcg
    38161 atgaagttcg ccgatttcgc cgtctggtca gaggcgcgtt ttagcggaat ggtcaaaacg
    38221 gcgctgacgc tggcagtaac gaccacctta aaggaattaa cgccgtgaaa attgaattaa
    38281 cggtgaatgg gctgaacgtg caagcgcagt accatgatga agagatagaa cgcgtgcata
    38341 aaccgttgct gcgtatgctg gcggccttac agacggtaaa tccgcagcgc cgaaccgtgg
    38401 tttttctttg cgctccgccg gggacgggta aatctaccct aaccaccttc tgggcatatc
    38461 tcgcgcaaca agacccggag ctgcccgcta ttcagacgct cccgatggat ggttttcacc
    38521 attacaatag ctggctggat gcgcatcaat tgcgcccctt caaaggcgca ccagagacat
    38581 ttgacgttgc gaaactggcg gaaaatctgc gccaggtcgt ggaaggggat tgtacgtggc
    38641 cgcagtacga tcgacaaaag catgatcctg ttgaagatgc gttgcacgtt accgcaccaa
    38701 taatcatcgt cgaaggaaac tggctattgc tggatgacga taactggcgc cagctagcgc
    38761 aattttgtga tttctcgatc tttatcaatg cgccagctac tgcgttgcgg gaacgactgg
    38821 tggggcgcaa gctggcggga gggttatcgc tggcagacgc agaagctttt tatgaccgta
    38881 ccgacgggcc gaatgttcgc cgggtgctgg aagagagtct gcctgcaaat ttgaccttaa
    38941 tgatgaccgc tacgggtgaa tatcgtctgg tggattaatg ttcctgatta gaccatcttc
    39001 cagtcgcacc acacgagaga aatgacgccg ggtaaatgcg gcgtcatgac tgattgctac
    39061 gacgctcgtg cctcgctggc ggcacttctc cagccagctt tcaaacacgc tcaaccaatt
    39121 ttcgtcaaaa tcccgactcg gctcgtccag cagcaatagt ggcggagaga gtgcttccag
    39181 gcaggcgacg gcgaccatcc gtcgctgcgc actatgcaga tccaacgggt gcgcgctggc
    39241 agtgtctgct aactgacaac attgcagggc agcatgggta cgttgagtga tttcatccgc
    39301 cgggcatttt tgcagtttca aaccaaatgc gatctcgtca gcgacggtgc tgtgaaaaag
    39361 ttggttttcc gcttcctgaa acagtacgcc aatctttgcc gcgcgctggc ggtttttcag
    39421 gtttttcatc gcctgttgtt gcagcataac cgtaccggct gtgggcgtaa gaagacctgc
    39481 cattactcgt aacagcgtcg atttccctgc gccattgtcg ccggtcaacg ccaaccattc
    39541 accttgtttg agttgcagag aaatgtcgca aagacaatcc gttgccgcgc caggccagcg
    39601 ataagatatc tgatttaacg ttaacatagt ggctggagcg ctccgttttg caattgccac
    39661 gcttgctggc agtagttcag gaaaggtgtg cgatgacgtt caaaaagaat aatcagcgaa
    39721 ccacgttcaa acgcccagtg ttgtaagcgc tgcaacagca tttcgctggc ctgcggcgtc
    39781 aggcggctaa aggcttcatc aagaattaat agctttggct gcatcgcgat ggcgcaggcg
    39841 atgaccacgc gctgtgtctc tccaccggag agtgttgccg ggtgacgatg gcgcaacggc
    39901 tggcactcgg tcagagtcag ggcggcatca atccgcgcca taatctctgc ttcagcaaga
    39961 cacagatttt ctggcccaaa ggcaacttct tcttccacgc taaaggtaca gccggagagc
    40021 tgcaaatagg gcgattgctg gacgagctgg atggtggcgg attgttcatt aagcgggaga
    40081 tgcccaatgg gcgtaccgag gagcgtcccc gtgcctgtta tctctcctgg cagaaagtcc
    40141 ggataccagc ccgccatcag ctgtgccagc gtacttttgc cactgccgtt gtcaccaaag
    40201 atggcgacca tccccggttt gacatagtga aagtcataac agaacggagg atgcgttgag
    40261 tgtgtcgggc aataacggaa ctgttctaac gtaaccatat ccacgctccg aattctgtca
    40321 gcatcagcaa gatcatggtg taacgcgcta cgcgttgtag ggtgctatcg gctggtgccc
    40381 acaacgttgt tcgtcggttg tggatccgaa atgcccgcat atccagcgcc gcaccacgga
    40441 cagcaagatc gtttagcgca ttgtgtgtta gcggaatagt taacgcgggc atcgcacgta
    40501 aacgctgata ccagccctca tccagcggca cgccgcgggc acgctgcgct tcatgaataa
    40561 tcgctaattg ccgttttaac tgctcaacga ccaataacgg cccggcaaac agataagcga
    40621 cgcccggcgg caggcgagaa gcaaacagag cgcggataaa acattgcacg gggacgtact
    40681 gcatccacaa ctgtgaggtt gaaacaatcg ccagaatccg caaccagagc gtaatagcat
    40741 gcgtccaacg ctccggcgaa cgcggcgtgc cgcttagcca ttctgttagc cagccaccgt
    40801 gtaccagcca cagcccggct cccagcgaga acatcagcca gacgacatat tttgcccgcc
    40861 gccgcgtggc ctttagcgca ataaggcaaa aaaaggtcgc cgcgctatag atcggcaaaa
    40921 tggtctgcgc gggcaggata agcgtggtgc aggccgccaa tgcccatagt gttaatgagg
    40981 taaatggatg catttagcgc acggcagcca ttgccggaaa atggcgcgtg gtgcgaatcg
    41041 gcagctggcg cagtaaaagc cagacaatca ccgctgtaag agttttatcg accaggttcg
    41101 cgcctatcac cgtaatcgcc accgactcca ccaggttttg ccccatcgag tgcatccagg
    41161 caacgaacaa atcggctccg ctaccggtga caccgccgaa cagcgcagta cgcagcggta
    41221 cagcgacgac cgttaccgca agcgtaataa tcacgccgct gacaacgact tttggcaggg
    41281 tgcgaaacca acccgctcgc gccagccaac cggcgaccag gccgatgacc atcgcgacgg
    41341 gggcaaaagc ggcagcgatc gggtcggtca gtaatcccca gagtaaattg gtcagtaagc
    41401 cggtcagcat cccgatcacc ggaccaagca gtaccgcgct aataagtgtg ccgatggagt
    41461 cgagaaatat aggtaatttg accatgctgg ccagttgccc gccgatcata ttgatggcga
    41521 tagatataac gatcaacacc agtgcctgac tggaaaagtg gctacgcgcc ataagaaaac
    41581 ctctctgaaa gtagttattt aacgggggaa atatgcaact cgtcgtaacc agaaagcgcg
    41641 caaggttggc ggctaaacgc cagttcctca aggctaccga tcaccagttc aagcgtggtc
    41701 cgcacggtac agccgggcat catatgaccg ccaagcatcg tgccgtgtgg atcggaaacg
    41761 cagagatgaa gatgctcacc actctgttcc aacgtgccgt ttagtgagat cacttcgaat
    41821 gtaccgttca ggagcgtggt gccttcttgc ccggcatagc gcaaggcgat gtcagtcagg
    41881 ctaccggtac agcctgcaat ccaggcggca tgaagttgct gctgctgtgc aaaagcgtgt
    41941 agctgggaaa aaacttcctg cccggggagg aggcgcagtg cgtaaaagcg tgcggaggaa
    42001 gaatatgggt agggcaatgt catcattgga gtactccgaa actcggacgt attacggata
    42061 atatcgacgc tgcatcagtc atattctgat ataagtcagg gtttgtgacg gcacaacgaa
    42121 aagattttgc tacgcttatt cgtgattctg caaagtggaa taaattccac aggggcgggg
    42181 ctgaaaacgt gacgtaagtc agataattgt tttctttgac tggacaatca ttccttttat
    42241 tccacgtttc gcttatccta gctgaagcgt ttcagtcgat taaatgttcg acaattaacc
    42301 aatcagtcgc agtttgcgac aggtaaggtt tccccggacg atttgctgga ttactctgtc
    42361 tggcaaattt gctcagggaa aaccttgcag gagatctatg accgtacgcg tagcgataaa
    42421 tggcttcggt cgcatcgggc gtaatgtggt tcgtgctttg tatgaatccg gacgccgggc
    42481 ggaaattacc gtggtggcaa tcaacgaact ggcggatgct gcgggcatgg cgcatttgtt
    42541 gaaatacgac accagccatg gccgttttgc atgggaagta cgacaggaac gcgatcaact
    42601 ttttgttggt gatgacgcca tccgcgtatt gcatgaacgt tcactgcaat cgctcccctg
    42661 gcgtgaactt ggcgttgatg tagtcctcga ctgcaccggc gtatatggct cccgcgagca
    42721 tggcgaagcg cacattgccg ccggggctaa aaaagtgctc ttttcacatc ctggcagtaa
    42781 cgatctcgac gcgaccgttg tttacggcgt caatcaggat caacttcgtg cggagcaccg
    42841 catcgtttct aacgcttcct gtaccacgaa ttgcataatt cccgtcatca aattgttaga
    42901 tgatgcgtac ggtattgagt ccggcactgt gaccacaatt cactccgcca tgcacgatca
    42961 acaggttatt gatgcatacc atcctgacct gcgtcgcacc cgggcagcca gccagtcgat
    43021 cattccggtc gatactaaac tggccgccgg tatcacacga ttttttccgc aatttaacga
    43081 tcgctttgaa gcgattgcgg tacgtgtgcc aaccataaat gtgacggcaa tcgatttaag
    43141 cgtgacggtg aagaaacctg taaaagccaa tgaagtcaac ctgttgctgc aaaaagcagc
    43201 acaaggtgca tttcatggta tagttgacta tacggaattg ccgttggtct ctgtagattt
    43261 taaccacgat ccgcacagtg ccattgtcga tggcacccaa acccgggtca gtggcgcaca
    43321 cctgatcaaa acgttggtct ggtgcgataa cgaatggggc tttgctaacc gaatgctcga
    43381 cacgacgtta gctatggcta ctgttgcttt caggtaagac gcaagcagcg tctgcaaaac
    43441 ttttagaatc aacgagagga ttcaccatgt ctgtaattaa gatgaccgat ctggatcttg
    43501 ctgggaaacg tgtatttatc cgtgcggatc tgaacgtacc agtaaaagac gggaaagtaa
    43561 ccagcgacgc gcgtatccgt gcttctctgc cgaccatcga actggccctg aaacaaggcg
    43621 caaaagtgat ggtaacttcc cacctgggtc gtcctaccga aggcgagtac aacgaagaat
    43681 tctctctgct gccggttgtt aactacctga aagacaaact gtctaacccg gttcgtctgg
    43741 ttaaagatta cctcgacggc gttgacgttg ctgaaggtga actggttgtt ctggaaaacg
    43801 ttcgcttcaa caaaggcgag aagaaagacg acgaaatcct gtccaaaaaa tacgctgcac
    43861 tgtgtgacgt gttcgtaatg gacgcattcg gtactgctca ccgcgcgcag gcttctactc
    43921 acggtatcgg taaattcgct gacgttgcgt gcgcaggccc gctgctggca gctgaactgg
    43981 acgcgctggg taaggcactg aaagaacctg ctcgcccgat ggtggctatc gttggtggtt
    44041 ctaaagtatc taccaaactg accgttctgg actccctgtc taaaatcgct gaccagctga
    44101 ttgttggtgg tggtatcgct aacaccttta tcgcggcaca aggccacgat gtgggtaaat
    44161 ccctgtacga agctgacctg gttgacgaag caaaacgtct gctgaccacc tgcaacatcc
    44221 cggttccgtc tgatgttcgc gtagcaaccg agttctctga aaccgctccg gctaccctga
    44281 aatctgttaa cgacgtgaaa gctgacgagc agatcctgga tatcggtgat gcttccgctc
    44341 aggaactggc tgaaatcctg aagaatgcga aaaccattct gtggaacggt ccggttggcg
    44401 tgttcgaatt cccgaacttc cgcaaaggta ctgaaatcgt ggctaacgct atcgcagaca
    44461 gcgaagcgtt ctccatcgct ggcggcggcg acactctggc agcaatcgac ctgttcggca
    44521 ttgctgacaa aatctcctac atctccactg gcggcggcgc attcctcgaa ttcgtggaag
    44581 gtaaagtact gcctgcagta gcgatgctcg aagagcgcgc taagaagtaa aaaatcacag
    44641 ggcagggaaa cctgcccttg tttcagcgcg cttttagagc acgcattttt tcatactcta
    44701 aataattcga gttgcaggaa ggcgacaaga gagtgaatcc ccaggagctt acataagtaa
    44761 gtgactgggg tgagcgaacg cagacgcagc acatgcaact tgaagtatga cgagtataag
    44821 gcccgacgat acaggacaag agacatgtct aagatttttg atttcgtaaa acctggcgta
    44881 atcactggtg atgacgtaca gaaagttttc caggtagcaa aagaaaacaa cttcgcactg
    44941 ccagcagtaa actgcgtcgg tactgactcc atcaacgccg tactggaaac tgctgctaaa
    45001 gttaaagcgc cggttatcgt tcagttctcc aacggtggtg cttcctttat cgctggtaaa
    45061 ggcgtgaaat ctgacgttcc gcagggtgct gctatcctgg gcgcgatctc tggtgcgcat
    45121 cacgttcacc agatggctga acattatggt gttccggtta tcctgcacac tgaccactgc
    45181 gcgaagaaac tgctgccgtg gatcgacggt ctgctggacg cgggtgaaaa acacttcgca
    45241 gctaccggta agccgctgtt ctcttctcac atgatcgacc tgtctgaaga atctctgcaa
    45301 gagaacatcg aaatctgctc taaatacctg gagcgcatgt ccaaaatcgg catgactctg
    45361 gaaatcgaac tgggttgcac cggtggtgaa gaagacggcg tggacaacag ccacatggac
    45421 gcttctgcac tgtacaccca gccggaagac gttgattacg catacaccga actgagcaaa
    45481 atcagcccgc gtttcaccat cgcagcgtcc ttcggtaacg tacacggtgt ttacaagccg
    45541 ggtaacgtgg ttctgactcc gaccatcctg cgtgattctc aggaatatgt ttctaagaaa
    45601 cacaacctgc cgcacaacag cctgaacttc gtattccacg gtggttccgg ttctactgct
    45661 caggaaatca aagactccgt aagctacggc gtagtgaaaa tgaacatcga taccgatacc
    45721 caatgggcaa cctgggaagg cgttctgaac tactacaaag cgaacgaagc ttatctgcag
    45781 ggtcagctgg gtaacccgaa aggcgaagat cagccgaaca agaaatacta cgatccgcgc
    45841 gtatggctgc gtgccggtca gacttcgatg atcgctcgtc tggaaaaagc attccaggaa
    45901 ctgaacgcga tcgacgttct gtaagtcatt cctttctgct tatctcaagg cccgctctgc
    45961 gggtcttttt ttcgccaaaa gcaaaattgc ctgatgcgct atgcttatca ggcctacacg
    46021 tatcctgcaa tatattgaat ttgcaggttt ttgtaggccg gataaggcgt tcacgccgca
    46081 tccggcatga caaagcgcac attgatatct gtctgagccc cgctctgcgg ggattttttt
    46141 gccaaaagca aggtgattca gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta
    46201 ttgtttaccc ttgtcagact gcccgtcata aggcggcgga gtgtatttct ccattttgag
    46261 tcagttgaaa aggaatattg aatggaagat ttgaatgttg tcgatagcat aaacggcgcg
    46321 ggaagctggc tggtagctaa ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg
    46381 gcactcgcga tcatcatcgt tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat
    46441 cgcctgatga tctcccgtaa aatcgatgcc actgttgctg attttctttc tgcattagtc
    46501 cgttacggta ttatcgcctt tacgctaatc gcagccctgg gacgcgtggg tgtacaaaca
    46561 gcgtcagtca ttgctgtact cggtgccgca ggcttagctg ttggtctggc tttgcagggg
    46621 tcactttcta acctggccgc tggcgtgtta cttgtcatgt tccgcccgtt ccgtgccgga
    46681 gaatatgttg acctcggcgg cgtagccggt actgtgctga gtgtgcagat tttctccacc
    46741 accatgcgta ctgcagacgg taaaattatc gttattccga acggtaaaat tattgccgga
    46801 aatattatta acttctcccg cgagccagtt cgccgtaacg aatttattat tggcgtggcg
    46861 tatgattccg atatcgatca ggttaagcag atcctgacca atattatcca gtctgaagat
    46921 cgcattttga aagatcgcga aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt
    46981 aatttcgtgg tccgcgtctg gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg
    47041 ctggagcgta ttaaacgtga atttgatgcc gccggtatca gcttcccgta cccgcaaatg
    47101 gatgtgaact ttaagcgggt gaaagaagac aaagctgcgt aatcaacgct gaggccagat
    47161 aatactctct ggcctctctc ttattagtta ttctgattgc caattaatat tatcaatttc
    47221 cgctaataac aatcccgcga tatagtctct gcatcagata cttaattcgg aatatccaac
    47281 gtgttttctt attactttca aggtcttgct cttggggcag ctatgattct gccgctcggc
    47341 ccacaaaatg cttttgtgat gaatcagggc attcgtcgtc agtaccacat tatgattgcc
    47401 ttactttgcg ctatcagcga tttggtcctg atttgcgcgg ggatttttgg tggtagcgcg
    47461 ttattgatgc agtcgccgtg gttgctggcg ctggtcacct ggggcggcgt agtcttcttg
    47521 ctgtggtatg gttttggcgc ttttaaaaca gcgatgagta gtaatattga gttagctagt
    47581 gccgaagtcc tgaagcaagg cagatggaaa attatcgcca ccatgttggc agtgacctgg
    47641 ctgaatccgc atgtttacct agatactttt gttgtactgg gcagccttgg cgggcagctt
    47701 gacgtcgaac caaaacgttg gtttgcgctc gggacaatta gcgcctcttt cctgtggttc
    47761 tttggtctgg ctattctcgc agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag
    47821 cgcattatca atctggtagt gggatgtgtc atgtggttta tagccttgca gctggcgaga
    47881 gacggtattg ctcatgcaca agccttgttc agttaggcgc tatctgatgg aaaaataaaa
    47941 cagaggcgct aagcttgcct ccagagatcc tgaattttgg gcaagtcaat taacacggag
    48001 agactaacgt gaagttcaaa gttatcgccc tggcggcatt aatgggtatt agcgggatgg
    48061 cagcgcaggc taacgaattg ccggatggac cgcatattgt cacctccggt acggcaagtg
    48121 tggatgcggt gccagacatt gccactcttg cgattgaagt taacgtggcc gcgaaggatg
    48181 ccgctacagc caagaaacag gcagatgagc gcgtcgcaca atacatttcc ttccttgaac
    48241 tcaatcagat cgcgaaaaaa gatatcagct cagcgaattt acgcacccag ccagattatg
    48301 attatcagga tggtaaaagt atcctgaaag gctaccgcgc tgtgagaacg gtggaagtca
    48361 cgctccgtca gttagacaaa ctgaattcct tgctggatgg cgcgctgaag gcgggtctta
    48421 acgaaattcg ttcagtgtcg ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc
    48481 gtaaggcagc gattgataac gcgattcatc aggcgcagga actggcgaac ggctttcatc
    48541 gtaaactggg gccggtatat agcgtgcgct accatgtttc caactatcag cccagcccaa
    48601 tggtgcggat gatgaaagcc gatgccgcgc cggtgtctgc ccaggaaact tacgagcagg
    48661 ccgctattca gtttgatgat caggtcgatg tggtcttcca gttagaacct gtggatcaac
    48721 aacccgctaa aacacctgca gcacaataat acttgtagcc cgacgtattc gtcgggctaa
    48781 tgcctttacc cgatattgcc tcctttttct gtaattttct gtaaaaccat gacgtaatca
    48841 cctatctttg ctcgcggttt catcaaggat aggctgactg aaacggttaa gcggacaagg
    48901 atgcggggag gggggatgga catttttatt tcgaaaaaga tgcgcaattt cattttatta
    48961 gcgcaaacca ataatattgc ccgggcggca gaaaaaatcc atatgacggc ttcgccattt
    49021 ggtaaaagca ttgccgcgct ggaagaacaa attggctata cgctatttac ccgcaaagat
    49081 aataatatca gcctcaataa agccggtcag gaactgtatc aaaaactgtt cccggtttat
    49141 caacgacttt ctgcgattga taatgaaatc cataactcag ggcgtcgttc acgagaaatt
    49201 gtgattggca tagataatac ttatccgaca attattttcg atcagttgat tagtctcgga
    49261 gataagtatg aaggcgtgac cgcccagcca gttgagttca gcgaaaacgg cgttatcgat
    49321 aatctttttg accgtcagtt agattttatt atctccccac agcatgtatc tgcacgagtt
    49381 caggaactgg aaaacctgac tatcagtgaa ttaccaccgc tgcgattgaa ctttctcgtt
    49441 tctcgccgtt atgaagagcg tcaagagcaa gagctgcttc aggagctgcc ctagctacaa
    49501 atgcgctttc aaaaccgggc taacttcgaa gcaatgattg atgcgaatat gcgaccttgt
    49561 gggatcaatc ccaccattat ttatcgtccc tacagtttta tggccaaaat cagcgccgtg
    49621 gagcgcggac attttctgac ggtgatcccg cattttgcct ggcgactggt gaatccggca
    49681 acgctgaaat attttgatgc gccgcacagg ccgatgtata tgcaggaata tctttattca
    49741 atcagaaatc atcgttatac cgccacgatg cttcagcata ttgctgaaga tcgtgacggg
    49801 acaaaccatt aacccagcat cgagccggtt gcaattaaat tacggtgtaa gtcgaagacg
    49861 tggctaagat cgtggtgaat atgtccgccg ggcgcatttt ccagatagcg atgcagataa
    49921 tcccgataca taggatgtgc acaattatca atgatagtgc gggcgcgttg aagcggggaa
    49981 agaccgcgca gatcggcgat cccttgttcg gtgatgatca ctttgacgct gtgttcgttg
    50041 tgatcaacat ggctgcacat tggcacgacg gttgagatct tgccttcttt agcaatcgac
    50101 ggagccataa agatcgacag atacgcgttg cgttcaaaat caccgctgcc gccgatgccg
    50161 ttcatcagat cgaccccggc tacgtgcgtt gagttggcat gcccgtaaat atcaaactcc
    50221 aggccgacgt tcagagcgat gacgcccaga cgacggatga tttccgggtt attggaaatc
    50281 tcctgcggac gcaacacaat gcggctggca aagtaatcca tattgtcgta aatcttgcgc
    50341 agggaatcgg ccgagattgt caggctggag gcgctggccc cgctgatttt gccggtttcc
    50401 agtaaatgca ccaccgattc ctgtagcact tccgaataca tcataaacgg aggaatttcc
    50461 gggttttccc ccagacgcgc cattaccgca ttattgatat tgcccacgcc actttgcagc
    50521 ggcagaaatt ccggcggaat acgcccatgc gccatttcct gcaataagaa cgtgaccacg
    50581 ttatcggcaa tctgctggca catgggattt tgcttatcca gcatattacc ggcgtcgggc
    50641 aagttggttt ccacgacggc gacaatcttt ttcggatcga tttgcacata gcgggtaccg
    50701 acgcgatcca ttgcatggaa gatcgacacg ctattgcgcc gcggtggcgc gccaggaatc
    50761 acaatatccg ccagttctgc aacgcgcgga tcgtgatagt gattgagttc aatgatcact
    50821 ttcttcgctc gcagcagcca ggtcggcgca ttaccgatcc cgctggttaa ccagactcga
    50881 ccatctggtg ccagtgccga tgcttcaatg acggcaacat caatgtcgcc gaagaaaccg
    50941 taattgacca tttgcgccac ttcgctcaaa tgcaggtcaa cgaaactcac cgcgccctga
    51001 ttgatctttt tacgtaaacc ggacgatgtt tgatatggcg cacgccagga aacagcatcg
    51061 gcgtcagaaa gtacatcgtc agcggcggcg ctgattgacg cccccgtcag caggcgaatt
    51121 tgatacggct ttttggcctc atgctgttcg ttagctctgc gggcaatcgc ggtgggtagg
    51181 gctttcggcg aacccgctgg ggtaaagccg ctaaatgcca ccatgtcgtt atgctggata
    51241 atttctgccg cttcatcggc ggtcatcctt gtccactgag tttccatttt tactccttcg
    51301 ttcgcaggga ttaatgacca acgaaattag gtttacgttt ttcgaggaaa gcgttcatcc
    51361 cttcctggta atcttcgctg tcataaaccg cgcggcgcat cccctgaata cgttcaaatt
    51421 catcggagtt catggtgtgt gcttcgccca gtacacgcag ctcttctttg ataacggcaa
    51481 tagctaacgg cgctttctca gagatgtggt gcgccatttg taaggtgaaa tcttccagtt
    51541 cttccacttc cacaacatgg ttgaggatgc cgacagccag cgcgcgctgg gcggtgatgg
    51601 gcgaagcggt aaaaatcagc tctttgacaa tgtggaagcc cgcgtcgcgg gtcaggttgt
    51661 gaatgccgac caggttatac gggacgccga ggtttacagg cgtcattgag aaggttgagg
    51721 tactggcggc gataatcaga tcggaactca tgatcatttc aaatgcgcca ccccaaacac
    51781 taccttccac catcgaaata atcggtttcg ggaatttttg gatcatgcgg gtgatttgac
    51841 gcaatggatc atcataggag agcggatcgc gaccgccaga cggcagttcg tgaatatcgt
    51901 gacctgcgga gaagactttg gatccactcg gcgcgcgcaa aatgatacag cgaatttccg
    51961 gccggttgag atcgcttaac gcctgcataa gatcatcaat aaagacttta cttaaggcat
    52021 taagttttcg gccatagtta aactcaatga ccgccacttt gttgatagtg acaacgttaa
    52081 catactgata agacataaaa attcctttaa tcaaaatatt gcgtctggat aaattcactg
    52141 agctgccgca ggccggtgcg cggtgagagc gtattgtttt tgaccgctaa aagcgtctgg
    52201 cggtaatagc gatcgaaatc ttcattcgcg aacaggtgat tcagtacttc ttcttcggtc
    52261 tgcttacgca gccattccac cgattgttgt tgccgcactt gttgtaaacg accactggca
    52321 gttagcgcgg ttttgaagtc gatgatggcg tgccagatct catcgattcc acgtttttcc
    52381 agtgcgctac aagtcagaac ccgtggctgc cattcgtcgt atttacgtcg cagaatatgc
    52441 agggcactct cgtacatatg ccgggcaatg gcgacattgg tatggttatc gccatcgtct
    52501 ttgttgataa cgatcagatc agccacttcc atcagccctt ttttaatgcc ctgcagatca
    52561 tcgccgccac cggcaatttg caacgagata aaacagtcca ccatgcgggc gacttctgtt
    52621 tccgactgcc cgacgccaac cgtttcgaca atcactacgt cataacccgc tgcttcgcat
    52681 aacagcatta attcccgcgc tcgctgactg gcaccgccca gatgaccgga ggatggtacc
    52741 gggcgaataa acgccgcttc ggcacgcgcc aggtcattca tgcgggtttt atccccgaga
    52801 atgctaccgc cagtgaccgg gctgctggga tcgaccgcaa taaccgcgac ctttaatccc
    52861 tctcgaatca acaacatgcc aaaggcctca agaaaggtac ttttccccgc gccgggggtg
    52921 ccggtaacgc ccagtcgcag ggtgttaccg cagtacggca taatggcatc aagcagctgc
    52981 gtacttagtg cctgatgacg cgggtgacgg ctttccacca gcgtcatggc ctgggcgagt
    53041 gtggcatgct caccctgacg taagcggcga atactttctg ccagcgtggc ttcattaatc
    53101 atgatgctgg cttatcagat tcagtacgtc gcgcacactg tcgagcatag gtgtacctgg
    53161 accataaatc gccgccacgc cgcgctcttg caggaaggcg taatcctgcg gtggaatgac
    53221 gccaccggcg actacgcaga tatcttcgcg tccccatttt ttcagcgctt cgaccagttc
    53281 cgggatcagc gttttatgac cggcagccag tgaggatgcg cccactacgt gaacgtcatt
    53341 ttctactgcc aggcgggcga tctcttcagg tgtagagaac atcgggctta aatctacgtc
    53401 gaaaccgaga tcggaatagg cgctggcgat cactttcgcg ccgcgatcgt gtccatccag
    53461 gcccattttg gcgatcagaa tgcgcgggcg acgaccattg tcggcaagga actgctccgt
    53521 ttgcgcaaca atggcatcga actcggaggc cgatttctca gattgatgat agctttgcgc
    53581 aatcacgccg gtaacacact ggcttggcac cagataacgg tcgaaagcgg cttccagcgc
    53641 atcggaaatt tcacccaggg tggcgcgaac gcgagcggca ttaacagcgg cagccagcag
    53701 gttttcgtta tgctgtgcgg cgtgagtcag ggcgttcaac gcggcggtta cggcggcatc
    53761 atcacgggtg gcgcgaatgc gttccagcga agcaatttgc tcgttacgca ccatcacgtt
    53821 gtcgatctca agtacatcgg tttcgtcttc gtgatccagt ttgtacttgt tgacaccaac
    53881 gatgacacgc ttgccctggt cgatcagcga ctgttcgcgc gctgaggcct cttcgatcat
    53941 tcgttttggc agacctgctt cgatcgcttt cgccatgcca ccggcttcgt cgatctgttg
    54001 gataatagct ctggcttgtt tgacgatttg atcggtcagc gactcaatgt aataggatcc
    54061 ggccagtgga tcgacggtgc ggcagagttc tgattcttcc tggatgatga tctgggtgtt
    54121 gcgggcaatg cgtgctgaga aatcggtagg caaaccaagc gcttcgtcaa aggcgttggt
    54181 atgcagtgac tgagtaccgc ccagcgtcgc agccagcgct tcaatggtgg tgcggataac
    54241 gttgttatac ggatcctgtt cagtcaggct ccagcctgag gtctggcagt gggtacgcag
    54301 cgccagtgat ttcgggtcct gtgcgccaaa tccactgacc gcttcgctcc ataaataacg
    54361 tgccgcacgc aacatggcga cgttcataaa cagatccatg ccgatgccga agaagaacga
    54421 caggcgagga gcgaagtcat caattttcag tccggcagag attgctgctt tgatgtactc
    54481 aatcccatca gcgagcgtaa atgctacctg ctgcacgcag ttggcacccg cttcacccat
    54541 gtggtaaccg ctgatactga tggtattaaa tcgcggcatg ttgccggaac accaggcgat
    54601 gatgtcggcg ataatgcgca ttgacggttt tggcgggtaa atataggtgt tgcggcagag
    54661 gtactctttg agaatatcgt tttgaatggt gccggtcagt ttatcaggtg taacaccttg
    54721 ctcttccgcg gcgatgatat aaaacgccag tactggtagc actgcgccat tcatggtcat
    54781 cgaaaccgac attttatcca gcgggatctg gtcgaacagg actttcatat cttccacggt
    54841 gtcgatagcg acgcccgttt tgccgacgtc acccgccacg cgtgggttat cggagtcgta
    54901 gccacggtgg gtggcaaggt caaacgcaac ggaaagacct ttttgcccgg cggccaggtt
    54961 acggcgataa aaagcgttgg actcttttgc tgttgaaaaa ccagcatact gacggatggt
    55021 ccacggttgg gcggtataca tagtggcacg cgggccacga acgtagggcg gcaaaccagg
    55081 aagggtacct gtcacctcca gattatcgag atcggcttcg gtatacagcg gcttgatggc
    55141 gatcccttcc gcggtttgct gaaccagcga gtcgacagtt ttctcccgac ggctcaattc
    55201 cttgttggca agctgttgcc actcctgctc gttagacatt atttgctccg taaaacgcaa
    55261 tcaaaatgag ggtatttgcg cttacggtga gctttttacg tgggtttgtc gccagcaaaa
    55321 aaaatgcatt cagacgtcgt tttttgccgc ctgagatttg tctattttgt gatgtgattc
    55381 aaggaataca aaaagaagtg aggataatgc ctgatatgtg ctatcaggca ttatttgatg
    55441 gattaatcct gacgaaggac tttgtgacca taatcgagca acgcatcagt gactttacgc
    55501 atcatgcggc tttcaggagc aaagcggtgc cagtagagca tccggcgttg aaatagccct
    55561 ggcgttaagt caatcagttc accgctggct agttctttct cgatttgcag gtgcgggatc
    55621 atacagcagg tggtgccctg gcgagcaagt tgcacgaacg cttctgaaga attaacgata
    55681 tggcagggca cgctgcctgg aggcagatcg aagttttgct gcaaaaaggc ctggtgcata
    55741 tcgtcaagat ggtcaaacgc gaccactggc gctttcagta atgccgaacg cgttacgccg
    55801 ttagggaaat atttttcggc aaagggtttt gagctgacga acagatagtc gagcgcacca
    55861 agtttatcga caagacaact cggcagcgcc tgatgttgaa tactcaccgc gccgaccact
    55921 tcgccgcgac gcagacgttc ctgagtgcgg gtttcatctt ctacctgcaa gttgaggcgg
    55981 ataggcgaat cagccaatac aggagccagt gccggaagca accacgtcgc cagactgtcg
    56041 gcattgaccg ccagtgaaag cagcagcgga gtcgaaccgg tttgttcatc gcccagccac
    56101 tcttcttcca gcaactccac ctggcgcagc agtgccagca gtttttgccc ttgttccgtc
    56161 gggcgcggcg gtacggtacg caccaacagc ggctgcccga acatattttc cagttgctta
    56221 atgcgctgtg agacggctga ttgtgtaatg cacagctttt gtgccgcgcg ctcaaatcct
    56281 cgttcacgta tcaccgcatc cagtgcctgt aatgttctgt agtccgggcg tttcattgct
    56341 gttgtctgct cctggtaaat gctttttcct gcactatgac acaattttgt gtcaggttgc
    56401 atataccgca gccgtcggcg ggtaacattt gtatcaatgg ttaaacgcgc agcttaaaag
    56461 atcagggatt tgcgctattg catttcgctt agcaacttcg cgccctggtt atgttgaatg
    56521 gcgtggcgtt attgcctctg ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt
    56581 tatttgcggg gcgaaagggg atgcccgcca ttgcgcgata ttttatggat gagttaacca
    56641 cgcggcttgc caacggggtc tgaatcgctt tttttgtata taatgcgtgt gaaatttcat
    56701 accacaggcg aaacgatcat gacgcaggat gaattgaaaa aagcagtagg atgggcggca
    56761 cttcagtatg ttcagcccgg caccattgtt ggtgtaggta caggttccac cgccgcacac
    56821 tttattgacg cgctcggtac aatgaaaggc cagattgaag gggccgtttc cagttcagat
    56881 gcttccactg aaaaactgaa aagcctcggc attcacgttt ttgatctcaa cgaagtcgac
    56941 agccttggca tctacgttga tggcgcagat gaaatcaacg gccacatgca aatgatcaaa
    57001 ggcggcggcg cggcgctgac ccgtgaaaaa atcattgctt cggttgcaga aaaatttatc
    57061 tgtattgcag acgcttccaa gcaggttgat attctgggta aattcccgct gccagtagaa
    57121 gttatcccga tggcacgtag tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg
    57181 gaataccgtc agggcgtagt gaccgataac ggcaacgtga tcctcgacgt ccacggcatg
    57241 gaaatccttg acccgatagc gatggaaaac gccataaatg cgattcctgg cgtggtgact
    57301 gttggcttgt ttgctaaccg tggcgcggac gttgcgctga ttggcacacc tgacggtgtc
    57361 aaaaccattg tgaaatgatc tgacggggga acctcccccg ttaaaaaaat tctcttcatt
    57421 aaatttggtg acatgtgtca cgtttttacc tggatattgt cgattgctct aaataaatcc
    57481 tctaaaccag catattcaac caagaaatac ctttgcgtga tatttcctca acatcgcgac
    57541 gcaaacgttc atattgccgc aatattattt tttgatatgt tgaaaggcgg atgcaaatcc
    57601 gcacacaaca tttcaaagac aggattgggt aaatggcaaa ggtatcgctg gagaaagaca
    57661 agattaagtt tctgctggta gaaggcgtgc accaaaaggc gctggaaagc cttcgtgcag
    57721 ctggttacac caacatcgaa tttcacaaag gcgcgctgga tgatgaacaa ttaaaagaat
    57781 ccatccgcga tgcccacttc atcggcctgc gatcccgtac ccatctgact gaagacgtga
    57841 tcaacgccgc agaaaagctg gtcgctattg gctgtttctg tataggaaca aaccaggttg
    57901 atctggatgc ggcggcaaag cgcgggatcc cggtatttaa cgcaccgttc tcaaatacgc
    57961 gctctgttgc ggagctggtg attggcgaac tgctgctgct attgcgcggc gtgccggaag
    58021 ccaatgctaa agcgcaccgt ggcgtgtgga acaaactggc ggcgggttct tttgaagcgc
    58081 gcggcaaaaa gctgggtatc atcggctacg gtcatattgg tacgcaattg ggcattctgg
    58141 ctgaatcgct gggaatgtat gtttactttt atgatattga aaacaaactg ccgctgggca
    58201 acgccactca ggtacagcat ctttctgacc tgctgaatat gagcgatgtg gtgagtctgc
    58261 atgtaccaga gaatccgtcc accaaaaata tgatgggcgc gaaagagatt tcgctaatga
    58321 agcccggctc gctgctgatt aatgcctcgc gcggtactgt ggtggatatt ccggcgctgt
    58381 gtgatgcgct ggcgagcaaa catctggcgg gggcggcaat cgacgtattc ccgacggaac
    58441 cggcgaccaa tagcgatcca tttacctctc cactgtgtga attcgacaac gtccttctga
    58501 cgccacacat tggcggttcg actcaggaag cgcaggagaa tatcggcctg gaagttgcgg
    58561 gtaaattgat caagtattct gacaatggct caacgctctc tgcggtgaac ttcccggaag
    58621 tctcgctgcc actgcatggt ggtcgtcgtc tgatgcacat ccacgaaaac cgtccgggcg
    58681 tgctaactgc gctgaacaaa atcttcgccg agcagggcgt caacatcgcc gcgcaatatc
    58741 tacaaacttc cgcccagatg ggttatgtag ttattgatat tgaagctgac gaagacgttg
    58801 ccgaaaaagc gctgcaggca atgaaagcta ttccgggtac cattcgcgcc cgtctgctgt
    58861 actaattccc cttctctgaa aattaacggg caggtcactg acttgcccgt ttttttatcc
    58921 cttctccaca ccgttgccgc gttgcgcatt ctactttgcg agtctgctcc agaaaataaa
    58981 tcaaagtcag atcaatgcgt tgtgtttatt taatggggat ttacgatggc gggacagcat
    59041 ggggctgtaa cggtgaagcc ctcaccgaag cgagggctta aaggagaagg gttatgatgc
    59101 gacttgtcat catactgatt gtactgttac tgataagttt cggagcttat taacagtcag
    59161 tctcagggga ggagtcatcc tcccttaccc ttactcacta aattaggtca aagaatcaac
    59221 gatgtcaatc agggcgatgc ggggtgcatc gccctgaacc gaactttttg atagccactt
    59281 catcgcccac gtttactgcc gtaattaatc ccttattcct gcaatgtatg tctgccttac
    59341 atgacgtttt acgagtgcgc tcgaagaaaa gatttaaagg tagatcaatg cgttgtggtt
    59401 atttaaagag agtttacgat ggcagggcag catggggctg taacggtaaa gccctcaccg
    59461 aagcgagggc ttgaaggaga agggttatga tgcgacttgt catcatactg attgtactgt
    59521 tactgataag tttgccagct tattaacagt cagtctcagg ggaggagtca tcctccctta
    59581 cccttactca ctaaattagg ttaaagaatc aacgatgtca atcagggcga tgcaggtgta
    59641 tcgcccttac cactcccaga ctttcgacgg tgtaaccacc gcaggaagag ggatatccca
    59701 ctcttcaacg gggagttttt ccaccaactg acaatcatgc gcataaccca ccggttgcgt
    59761 tttatagtgc tgccagtttt gtaaggtccg atcataaaaa ccaccgccca ttcccaggcg
    59821 ctgaccgtac tcatcaaagg cgaccagcgg tgtgatcagc acgtctaatc gggaaagggg
    59881 gagcacgtca cgcacatcca gttttggctc atggattttc aacctgttca tcaccagttc
    59941 gctttgcgga tggtaattca ggaacagcaa attaccggca ctaaagggat gcaaaactgg
    60001 aagatatacg cgcttaccgg cgcgccagag ttgttctata agcggctggg tgtcgagttc
    60061 accatcaaaa gagaggaata cagcgaccgt atgcgccatc accaccgggg gataagtcat
    60121 catccgggta gcggcttgtt gacccatttc ctgctgttgt tccggcgtta acgcacgacg
    60181 acgttgccga atcattttgc ggatttcttg tcgggataat gtcagtggga gttctggtag
    60241 ttgcgtcatg gctggtgcca gataagaagg gaatctccga gatgccgccg caggctgtaa
    60301 cccttgaacc cttggttcaa ggtgaatgtg tcgtcgcagt tttaaggctt ctcggacgga
    60361 ccgagcatgc tcaccaaccg cggagcgcca cattcttgtg gtatgaaata tcggctcagg
    60421 ggactggccc gcttgcgaac atctcagaga aattttgtct tcacggctac tctaccacag
    60481 taaaccgaaa agtgtcattc aaagttttgg ttagtttttt cggtgatgcg accttgttca
    60541 agtaacgctt gttctatggt ctgctgcagc atccgaatac gctgttccat acttgccgcg
    60601 tagtcacgag tcttcgcttt ttcttgcgct aactcatagc tgatattcaa tgcggcaatg
    60661 aagaccaact gttcagtatt tgtgactcta gtgcgttctt tcagatcttg caaccgttgg
    60721 ttcagatcgt ccgctgcctg attcaacgca tccctttggt caggcgggca gttcacacgc
    60781 agtgaacggc caaaaatttg gatatcgacg ggttgtgcag acatgccacc ttcctgctga
    60841 ttgactgcgc tgccttcgtc tttagaccca tggtctgcga aggggcgaca ctatagctac
    60901 cctgatgaga agagacaagc ccttttctgg tccaccaggg cccaaagtgg tagcatatca
    60961 tgaatattcc tccctttgac gacgaatgct tatgtctata cagaacgaaa tgcctggtta
    61021 caacgaaatg aaccagtatc tgaaccaaca agggacgggt ctgacccctg ctgagatgca
    61081 tggtttaatc agtgggatga tatgtggcgg taacgatgac agctcatggc taccgctact
    61141 tcacgacctg acgaacgaag gcatggcttt cggtcatgag ctggcacagg cattgcgtaa
    61201 aatgcactct gccaccagcg atgccctgca ggatgacggc ttcctttttc agctttatct
    61261 gcctgatggc gatgatgtca gcgttttcga tcgggctgat gcattggcag gttgggtcaa
    61321 tcacttcctg cttggtcttg gtgttacgca accgaagctg gataaagtga ccggcgaaac
    61381 cggtgaagct atcgacgatc tgcgtaacat tgcgcaactg ggttacgacg aagacgaaga
    61441 tcaggaagag cttgaaatgt cgcttgaaga gatcatcgaa tacgttcgtg ttgccgcgct
    61501 gttatgccac gacaccttta ctcatccgca accgaccgcg ccagaagtac aaaaaccgac
    61561 tctacactaa aaacaaaaaa cgtaaggaga gtgttatgag tgagatatcc cggcaagagt
    61621 ttcagcgtcg ccgtcaggcc ctggtggagc aaatgcaacc cggcagcgcc gcgctgattt
    61681 ttgctgcacc agaagtaaca cgtagcgccg acagcgaata cccctatcgt cagaacagtg
    61741 acttctggta cttcaccggc tttaacgaac cggaagcggt gttggtgctg attaaaagcg
    61801 atgacactca taaccacagc gttctgttta accgcgttcg cgacctgacg gcggagatct
    61861 ggtttggccg tcgcttaggc caggatgccg cgccagagaa actgggcgtt gaccgcgcac
    61921 tggcattcag cgaaattaat cagcaacttt atcaactact taatggtctg gatgtggttt
    61981 accatgctca gggcgaatat gcatatgctg atgaaatcgt gaacagtgcg ctggaaaaac
    62041 tgcgtaaagg ttcgcggcaa aatctcaccg caccggcaac gatgatcgac tggcgtcctg
    62101 ttgttcatga aatgcgcctg ttcaaatcgc cagaagagat tgccgtactc cgccgcgcgg
    62161 gagaaatcac cgccatggca catacacggg cgatggaaaa atgccgtccg ggaatgttcg
    62221 agtaccatct ggaaggcgaa attcaccacg aatttaaccg ccacggtgcg cgctatccgt
    62281 cctataacac cattgtaggc agcggtgaaa acggctgcat tttacactac accgaaaacg
    62341 agtgtgaaat gcgcgacggc gacctggtgt tgattgacgc aggctgcgaa tacaaaggtt
    62401 acgcaggcga tatcacccgt accttcccgg tcaacggcaa attcacccag gcccagcgcg
    62461 aaatctacga cattgtgctg gagtctcttg aaaccagcct gcgcctgtat cgtccgggaa
    62521 cctccattct ggaagtcacc ggtgaagtgg tgcgcattat ggttagcggc ctggtaaaac
    62581 tcggcatcct gaaaggtgat attgatgaac tgatcgctca gaacgcccat cgtcctttct
    62641 ttatgcatgg ccttagccac tggttaggac tggatgtcca tgacgttggc gtttatggtc
    62701 aggatcgctc gcgcattctg gaaccgggga tggtactgac cgtagagcct ggtctgtata
    62761 tcgcgccgga tgcggatgtg ccagaacaat atcgcggtat cggcattcgt attgaagacg
    62821 acattgtgat taccgaaacc ggcaacgaaa acctcaccgc cagcgtggtg aaaaagccgg
    62881 aagaaatcga agcgttgatg gctgctgcga gaaagcaatg agcgtaatca tcgtcggtgg
    62941 cggcatggcg ggcgcgacgc tggcgctggc tatttcccgg ttaagtcacg gggcgctgcc
    63001 ggtacatttg attgaagcga ctgcgccaga gtcacatgct catccgggct ttgatggacg
    63061 agcgatagcg ctggcggcgg gtacctgtca gcaactggcg cgcatcggcg tttggcaatc
    63121 tctggcggat tgcgcaactg ccatcaccac cgtgcatgtc agcgatcgag gtcacgctgg
    63181 atttgtcacc ctcgccgcag aagattacca actggcggcg ctgggacagg ttgtcgaatt
    63241 gcacaatgtc gggcaacggc tgtttgcatt gctgcgtaaa gcacctggcg taacgctgca
    63301 ttgccctgat cgcgtggcta acgttgcccg tactcagagt cacgttgaag tgacgctgga
    63361 gggtggcgag acgctgacgg gccgcgtgct ggtagccgct gatggcaccc attcagcgtt
    63421 agccactgtg tgcggcgttg actggcagca ggagccttac gaacaactgg ctgtgattgc
    63481 caacgttgcg acttccgttg cgcatgaagg gcgcgctttt gaacgcttta cgcaacatgg
    63541 cccgctggcg atgttgccga tgtctgacgg acgctgttcg ctggtctggt gtcatccact
    63601 ggaacggcgc gaagaggtgc tgtcgtggag cgacgagaag ttttgccgtg aactccagtc
    63661 ggcctttggc tggcgacttg ggaaaattac ccacgctggt aaacgcagtg cttatccgct
    63721 ggcgttaacc cacgccgcca gatctattac ccatcgtacc gtgctggtgg gcaatgcggc
    63781 gcaaaccctg cacccgattg ccgggcaagg gttcaacctc ggtatgcgag atgtcatgag
    63841 tcttgcggaa accctgactc aggcgcagga gcgcggagaa gacatgggtg attacggcgt
    63901 attgtgtcgt tatcagcagc gtcgacagag cgatcgcgaa gcaaccattg gcgtcacgga
    63961 cagccttgta catctttttg ccaaccgttg gacgccactg gttgtcgggc gcaacatcgg
    64021 gctgatgacg atggaattat tcaccccggc acgcgatgtg ctggcgcagc gcaccctcgg
    64081 ttgggtggcg cgttgaaggc ggttattaag gaataaacaa tgcaaagtgt tgatgtagcc
    64141 attgttggcg gcggcatggt ggggctggcg gttgcctgcg gcttacaggg tagcggctta
    64201 cgcgttgccg tactggagca gcgcgtaccg gaacctctgg cggcggatgc accaccacaa
    64261 ctgcgcgttt cggctatcaa tgccgccagc gaaaaattac tcacccgtct tggcgtctgg
    64321 caggacattc tctctcgccg agccagctgt tatcacggta tggaagtgtg ggacaaagac
    64381 agttttggtc acatctcgtt tgacgatcaa agcatgggct atagccatct tgggcacatc
    64441 gttgaaaact cagtgattca ctacgcgctg tggaacaaag cgcagcagtc gtcagatatc
    64501 actcttttgg cccccgcaga attacagcag gtcgcctggg gagaaaatga aaccttcctg
    64561 acgctgaaag atggcagtat gttaacggcg cgtctggtga ttggcgcgga cggcgctaat
    64621 tcctggttgc gcaacaaagc cgatattccg ctgactttct gggattatca gcatcacgcg
    64681 ctggtagcga ccatacgcac ggaagaaccg catgatgcgg tggcgcggca ggttttccat
    64741 ggcgaaggca ttctggcctt tttaccgctt agcgatccgc atctttgctc gattgtctgg
    64801 tcactgtcgc cagaggaagc gcagcggatg cagcaggcaa gtgaagacga atttaatcgc
    64861 gcgttaaata tcgcttttga taatcgcctg ggcttatgca aggttgagag cgcgcgtcag
    64921 gtgttcccac tgacggggcg ttatgcgcgc cagtttgccg cgcaccgtct ggcgctggtg
    64981 ggcgacgccg cgcataccat tcacccgctg gcggggcagg gggtaaatct tggctttatg
    65041 gatgctgcag agctgattgc tgaactgaaa cggttgcatc gtcagggtaa agacatcggg
    65101 cagtacattt atctgcgtcg ctatgagcgt agccgcaagc acagtgcggc gctgatgctg
    65161 gctggtatgc agggattccg cgatctgttt tctggtacca atccggtgaa aaaactgctg
    65221 cgtgatattg gtctgaaact ggccgacacg cttcctggcg ttaaaccaca acttatccgc
    65281 caggcaatgg gattaaacga tttgcctgaa tggctgcgtt aaaaatttct cttctgctgt
    65341 ttatttgata cccatcacac tttcatctcc cggttttttc gccgggagat tttcctcatt
    65401 tgaaataaac taatttcacc tccgttttcg cattatattt tctaatgtca cgaatttttg
    65461 attt
//
LOCUS       DAZFDC010000011        65389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFDC010000011 DAZFDC010000000
VERSION     DAZFDC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.011"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..686
                     /gene="comR"
                     /locus_tag="ACHJ48_000835"
     CDS             54..686
                     /gene="comR"
                     /locus_tag="ACHJ48_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO4706446.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(748..1287)
                     /gene="ycfJ"
                     /locus_tag="ACHJ48_000836"
     CDS             complement(748..1287)
                     /gene="ycfJ"
                     /locus_tag="ACHJ48_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4706447.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDEHRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1514..2818)
                     /gene="ndh"
                     /locus_tag="ACHJ48_000837"
     CDS             complement(1514..2818)
                     /gene="ndh"
                     /locus_tag="ACHJ48_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO4706448.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDRDAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVELTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKLLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3218..3760)
                     /gene="ycfP"
                     /locus_tag="ACHJ48_000838"
     CDS             complement(3218..3760)
                     /gene="ycfP"
                     /locus_tag="ACHJ48_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO4706449.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3783..4808)
                     /gene="nagZ"
                     /locus_tag="ACHJ48_000839"
     CDS             complement(3783..4808)
                     /gene="nagZ"
                     /locus_tag="ACHJ48_000839"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO4706450.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQVSLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4819..5643)
                     /gene="thiK"
                     /locus_tag="ACHJ48_000840"
     CDS             complement(4819..5643)
                     /gene="thiK"
                     /locus_tag="ACHJ48_000840"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO4706451.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5624..6265)
                     /gene="lpoB"
                     /locus_tag="ACHJ48_000841"
     CDS             complement(5624..6265)
                     /gene="lpoB"
                     /locus_tag="ACHJ48_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO4706452.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6279..6656)
                     /gene="ycfL"
                     /locus_tag="ACHJ48_000842"
     CDS             complement(6279..6656)
                     /gene="ycfL"
                     /locus_tag="ACHJ48_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO4706453.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6659..7018)
                     /gene="hinT"
                     /locus_tag="ACHJ48_000843"
     CDS             complement(6659..7018)
                     /gene="hinT"
                     /locus_tag="ACHJ48_000843"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO4706454.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7358..9547
                     /gene="fhuE"
                     /locus_tag="ACHJ48_000844"
     CDS             7358..9547
                     /gene="fhuE"
                     /locus_tag="ACHJ48_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO4706455.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9607..11040)
                     /gene="ptsG"
                     /locus_tag="ACHJ48_000845"
     CDS             complement(9607..11040)
                     /gene="ptsG"
                     /locus_tag="ACHJ48_000845"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO4706456.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11335..12132)
                     /gene="ycfH"
                     /locus_tag="ACHJ48_000846"
     CDS             complement(11335..12132)
                     /gene="ycfH"
                     /locus_tag="ACHJ48_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4706457.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12143..13147)
                     /gene="holB"
                     /locus_tag="ACHJ48_000847"
     CDS             complement(12143..13147)
                     /gene="holB"
                     /locus_tag="ACHJ48_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO4706458.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITALLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13144..13785)
                     /gene="tmk"
                     /locus_tag="ACHJ48_000848"
     CDS             complement(13144..13785)
                     /gene="tmk"
                     /locus_tag="ACHJ48_000848"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO4706459.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEIITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTRWVKELDA"
     gene            complement(13775..14797)
                     /gene="yceG"
                     /locus_tag="ACHJ48_000849"
     CDS             complement(13775..14797)
                     /gene="yceG"
                     /locus_tag="ACHJ48_000849"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO4706460.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYITVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAIASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14800..15609)
                     /gene="pabC"
                     /locus_tag="ACHJ48_000850"
     CDS             complement(14800..15609)
                     /gene="pabC"
                     /locus_tag="ACHJ48_000850"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO4706461.1"
                     /translation="MFLINGYKQESLSVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGITLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGGVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15729..16970)
                     /gene="fabF"
                     /locus_tag="ACHJ48_000851"
     CDS             complement(15729..16970)
                     /gene="fabF"
                     /locus_tag="ACHJ48_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO4706462.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17058..17294)
                     /gene="acpP"
                     /locus_tag="ACHJ48_000852"
     CDS             complement(17058..17294)
                     /gene="acpP"
                     /locus_tag="ACHJ48_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4706463.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17505..18239)
                     /gene="fabG"
                     /locus_tag="ACHJ48_000853"
     CDS             complement(17505..18239)
                     /gene="fabG"
                     /locus_tag="ACHJ48_000853"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO4706464.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILVQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18252..19181)
                     /gene="fabD"
                     /locus_tag="ACHJ48_000854"
     CDS             complement(18252..19181)
                     /gene="fabD"
                     /locus_tag="ACHJ48_000854"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO4706465.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQHGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19197..20150)
                     /gene="fabH"
                     /locus_tag="ACHJ48_000855"
     CDS             complement(19197..20150)
                     /gene="fabH"
                     /locus_tag="ACHJ48_000855"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO4706466.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20218..21288)
                     /gene="plsX"
                     /locus_tag="ACHJ48_000856"
     CDS             complement(20218..21288)
                     /gene="plsX"
                     /locus_tag="ACHJ48_000856"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO4706467.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21369..21542)
                     /gene="rpmF"
                     /locus_tag="ACHJ48_000857"
     CDS             complement(21369..21542)
                     /gene="rpmF"
                     /locus_tag="ACHJ48_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO4706468.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21594..22115)
                     /gene="yceD"
                     /locus_tag="ACHJ48_000858"
     CDS             complement(21594..22115)
                     /gene="yceD"
                     /locus_tag="ACHJ48_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO4706469.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22314..22898
                     /gene="yceF"
                     /locus_tag="ACHJ48_000859"
     CDS             22314..22898
                     /gene="yceF"
                     /locus_tag="ACHJ48_000859"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO4706470.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22999..23958)
                     /gene="rluC"
                     /locus_tag="ACHJ48_000860"
     CDS             complement(22999..23958)
                     /gene="rluC"
                     /locus_tag="ACHJ48_000860"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO4706471.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24531..27716
                     /gene="rne"
                     /locus_tag="ACHJ48_000861"
     CDS             24531..27716
                     /gene="rne"
                     /locus_tag="ACHJ48_000861"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO4706472.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGGEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETREGRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEETV
                     VAPVAEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27911..28864)
                     /gene="flgL"
                     /locus_tag="ACHJ48_000862"
     CDS             complement(27911..28864)
                     /gene="flgL"
                     /locus_tag="ACHJ48_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO4706473.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDNDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEVNGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28876..30519)
                     /gene="flgK"
                     /locus_tag="ACHJ48_000863"
     CDS             complement(28876..30519)
                     /gene="flgK"
                     /locus_tag="ACHJ48_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO4706474.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30585..31526)
                     /gene="flgJ"
                     /locus_tag="ACHJ48_000864"
     CDS             complement(30585..31526)
                     /gene="flgJ"
                     /locus_tag="ACHJ48_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO4706475.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTTGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31526..32623)
                     /gene="flgI"
                     /locus_tag="ACHJ48_000865"
     CDS             complement(31526..32623)
                     /gene="flgI"
                     /locus_tag="ACHJ48_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO4706476.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAIA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32635..33333)
                     /gene="flgH"
                     /locus_tag="ACHJ48_000866"
     CDS             complement(32635..33333)
                     /gene="flgH"
                     /locus_tag="ACHJ48_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO4706477.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33386..34168)
                     /gene="flgG"
                     /locus_tag="ACHJ48_000867"
     CDS             complement(33386..34168)
                     /gene="flgG"
                     /locus_tag="ACHJ48_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO4706478.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34306..35061)
                     /gene="flgF"
                     /locus_tag="ACHJ48_000868"
     CDS             complement(34306..35061)
                     /gene="flgF"
                     /locus_tag="ACHJ48_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO4706479.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADITPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35081..36286)
                     /gene="flgE"
                     /locus_tag="ACHJ48_000869"
     CDS             complement(35081..36286)
                     /gene="flgE"
                     /locus_tag="ACHJ48_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO4706480.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36311..37006)
                     /gene="flgD"
                     /locus_tag="ACHJ48_000870"
     CDS             complement(36311..37006)
                     /gene="flgD"
                     /locus_tag="ACHJ48_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO4706481.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37018..37422)
                     /gene="flgC"
                     /locus_tag="ACHJ48_000871"
     CDS             complement(37018..37422)
                     /gene="flgC"
                     /locus_tag="ACHJ48_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO4706482.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37426..37842)
                     /gene="flgB"
                     /locus_tag="ACHJ48_000872"
     CDS             complement(37426..37842)
                     /gene="flgB"
                     /locus_tag="ACHJ48_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO4706483.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRNATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37997..38656
                     /gene="flgA"
                     /locus_tag="ACHJ48_000873"
     CDS             37997..38656
                     /gene="flgA"
                     /locus_tag="ACHJ48_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO4706484.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38732..39025
                     /gene="flgM"
                     /locus_tag="ACHJ48_000874"
     CDS             38732..39025
                     /gene="flgM"
                     /locus_tag="ACHJ48_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO4706485.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39030..39446
                     /gene="flgN"
                     /locus_tag="ACHJ48_000875"
     CDS             39030..39446
                     /gene="flgN"
                     /locus_tag="ACHJ48_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO4706486.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39486..41021)
                     /gene="murJ"
                     /locus_tag="ACHJ48_000876"
     CDS             complement(39486..41021)
                     /gene="murJ"
                     /locus_tag="ACHJ48_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO4706487.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41131..42054)
                     /gene="yceM"
                     /locus_tag="ACHJ48_000877"
     CDS             complement(41131..42054)
                     /gene="yceM"
                     /locus_tag="ACHJ48_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4706488.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYCELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42056..42703)
                     /gene="yceH"
                     /locus_tag="ACHJ48_000878"
     CDS             complement(42056..42703)
                     /gene="yceH"
                     /locus_tag="ACHJ48_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO4706489.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42714..43298)
                     /gene="rimJ"
                     /locus_tag="ACHJ48_000879"
     CDS             complement(42714..43298)
                     /gene="rimJ"
                     /locus_tag="ACHJ48_000879"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4706490.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVCDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43534..44742
                     /gene="mdtH"
                     /locus_tag="ACHJ48_000880"
     CDS             43534..44742
                     /gene="mdtH"
                     /locus_tag="ACHJ48_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO4706491.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSVHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44806..45453
                     /gene="grxB"
                     /locus_tag="ACHJ48_000881"
     CDS             44806..45453
                     /gene="grxB"
                     /locus_tag="ACHJ48_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO4706492.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45587..46147
                     /gene="yceB"
                     /locus_tag="ACHJ48_000882"
     CDS             45587..46147
                     /gene="yceB"
                     /locus_tag="ACHJ48_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4706493.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVALTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46253..47299
                     /gene="pyrC"
                     /locus_tag="ACHJ48_000883"
     CDS             46253..47299
                     /gene="pyrC"
                     /locus_tag="ACHJ48_000883"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO4706494.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILHAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47373..47618
                     /gene="dinI"
                     /locus_tag="ACHJ48_000884"
     CDS             47373..47618
                     /gene="dinI"
                     /locus_tag="ACHJ48_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO4706495.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47908..48162
                     /gene="bssS"
                     /locus_tag="ACHJ48_000885"
     CDS             47908..48162
                     /gene="bssS"
                     /locus_tag="ACHJ48_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO4706496.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48277..49395
                     /gene="solA"
                     /locus_tag="ACHJ48_000886"
     CDS             48277..49395
                     /gene="solA"
                     /locus_tag="ACHJ48_000886"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO4706497.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFSLSRFQ"
     gene            49443..49556
                     /locus_tag="ACHJ48_000887"
     CDS             49443..49556
                     /locus_tag="ACHJ48_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO4706498.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49817..50383
                     /gene="yceJ"
                     /locus_tag="ACHJ48_000888"
     CDS             49817..50383
                     /gene="yceJ"
                     /locus_tag="ACHJ48_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4706499.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50387..50962
                     /gene="yceI"
                     /locus_tag="ACHJ48_000889"
     CDS             50387..50962
                     /gene="yceI"
                     /locus_tag="ACHJ48_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO4706500.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51004..52056)
                     /gene="trhO"
                     /locus_tag="ACHJ48_000890"
     CDS             complement(51004..52056)
                     /gene="trhO"
                     /locus_tag="ACHJ48_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO4706501.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVKTFRAQLYAFDSALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52281..53201
                     /gene="lpxL"
                     /locus_tag="ACHJ48_000891"
     CDS             52281..53201
                     /gene="lpxL"
                     /locus_tag="ACHJ48_000891"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO4706502.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53373..54599
                     /gene="mdtG"
                     /locus_tag="ACHJ48_000892"
                     /pseudo
     CDS             53373..54599
                     /gene="mdtG"
                     /locus_tag="ACHJ48_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            54682..55056
                     /gene="msyB"
                     /locus_tag="ACHJ48_000893"
     CDS             54682..55056
                     /gene="msyB"
                     /locus_tag="ACHJ48_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4706503.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(55057..55284)
                     /gene="yceK"
                     /locus_tag="ACHJ48_000894"
     CDS             complement(55057..55284)
                     /gene="yceK"
                     /locus_tag="ACHJ48_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4706504.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(55457..58000)
                     /gene="mdoH"
                     /locus_tag="ACHJ48_000895"
     CDS             complement(55457..58000)
                     /gene="mdoH"
                     /locus_tag="ACHJ48_000895"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO4706505.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(57993..59528)
                     /gene="mdoG"
                     /locus_tag="ACHJ48_000896"
     CDS             complement(57993..59528)
                     /gene="mdoG"
                     /locus_tag="ACHJ48_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO4706506.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            59921..61078
                     /gene="mdoC"
                     /locus_tag="ACHJ48_000897"
     CDS             59921..61078
                     /gene="mdoC"
                     /locus_tag="ACHJ48_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO4706507.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGTFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(61086..62567)
                     /gene="clsC"
                     /locus_tag="ACHJ48_000898"
     CDS             complement(61086..62567)
                     /gene="clsC"
                     /locus_tag="ACHJ48_000898"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO4706508.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMSEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(62509..63042)
                     /gene="ymdB"
                     /locus_tag="ACHJ48_000899"
     CDS             complement(62509..63042)
                     /gene="ymdB"
                     /locus_tag="ACHJ48_000899"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO4706509.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(63137..63448)
                     /gene="ymdA"
                     /locus_tag="ACHJ48_000900"
     CDS             complement(63137..63448)
                     /gene="ymdA"
                     /locus_tag="ACHJ48_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO4706510.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(63569..63901)
                     /gene="csgC"
                     /locus_tag="ACHJ48_000901"
     CDS             complement(63569..63901)
                     /gene="csgC"
                     /locus_tag="ACHJ48_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO4706511.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(63960..64418)
                     /gene="csgA"
                     /locus_tag="ACHJ48_000902"
     CDS             complement(63960..64418)
                     /gene="csgA"
                     /locus_tag="ACHJ48_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO4706512.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(64459..64914)
                     /gene="csgB"
                     /locus_tag="ACHJ48_000903"
     CDS             complement(64459..64914)
                     /gene="csgB"
                     /locus_tag="ACHJ48_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO4706513.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        15971 a        17353 c        16040 g        16025 t
ORIGIN      
        1 gtgattattt ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa
       61 ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg
      121 ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg
      181 cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca
      241 aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg
      301 aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg
      361 ccatcgccaa ctgctttacc ggcaaagaca ccccggcagg ctgcttcatg atcaacaact
      421 gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga
      481 tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga
      541 cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga
      601 tcagcgcacg cgaaggtgca tcgttggaaa aactgatgca gattgccgga acgactttgc
      661 gtttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga
      721 ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt
      781 actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc
      841 gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt
      901 acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt
      961 accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc
     1021 accaaactga tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg
     1081 atgttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg
     1141 tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg
     1201 ttcaaacacg ttcagactgg ccactgccgc tacgccaagc gcagctgcga caccaatccc
     1261 gatacccgcc aacattgatt tattcacggg aatacctcct tctttgcagt tatgccaatc
     1321 atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg
     1381 aaatacttaa ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt
     1441 atcaggccta ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc
     1501 cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca
     1561 ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata
     1621 gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt
     1681 tacccatcag gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt
     1741 tatactgata atttttcagc agcttaccgt tcatctgcgc cagaatgttg ttcattgcgc
     1801 aagtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg
     1861 ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtca
     1921 gttccaccac cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt
     1981 ctggcgcttt gatcccggct gcccatacca tcagatcagc ctcaatatat tcgccatctt
     2041 tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcaga acgcgaacgc
     2101 caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa
     2161 tacgttctcc cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac
     2221 cgtagctgtg cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg
     2281 cgccgccgcc gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt
     2341 tcaggaagag attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga
     2401 aaatgcagtt ctctttgaca cctggtgtat tgaaatcgtt agaggtgcta cccagcgcca
     2461 ttaccagggt gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc
     2521 gcagttctgc aatagtgatt gttttcgcgt cacgatcaat atcaatgacg gaacccagct
     2581 ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat
     2641 caagcgagcc agtcgctact tcgtgcagca gcggtttcca caggtggctg tggttacgat
     2701 cgaccagcgt aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca
     2761 tttccagccc accagcaccg ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg
     2821 tgaccccctt aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt
     2881 aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat
     2941 tggtcatacc aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc
     3001 gggtgagatc aaaaaacaga gtttattgtt tattatttga gcgaatttgt gagagtgata
     3061 tagcgtgagg ggtgtatgta gggcgttcgg taggtctgat aagaggcgca tcaggcattg
     3121 atgctcgact gtcggatgcg gcgtgaacgc cttatccgac ttacagaatt cggagcgcat
     3181 caggtaccgg caaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt
     3241 taatgcgctg caaatgcggg gagatattct tgaatttgtg cgtctgctct tcgtcccaga
     3301 caatctcgta ataatgatgc aactcttcag atgtccgctg gctgttaagc gcttcatcat
     3361 tacgcgacaa aatcaccagg caacgatcgc gattcttctc gcggaagttg gtcacacact
     3421 tagtggcaat atcggcatac tcttccgggc gatcaatctt gccttccatg ttctcataag
     3481 ggaacaaatt agggttgaag atcacctggc ggatatcgca aagaaaacca atccgttccg
     3541 cccagtatcc gcccagacca acgccgcaaa tcaggggacg ctcgtcgacg ttcagttgca
     3601 acattttgtc cacttctttg agcagatgct gcatatcatg tttcggatgc cgcgtactgt
     3661 agcttatcaa gcgtacatcc gggtcaataa actgcaattg taagactttc tcgtggttac
     3721 ccggactgtt agagtcaaaa ccgtgtaaat agataatcat cgcttcctca cataagccag
     3781 ggttagtgac ctgctttctc ttcctgccag cgttcatgta actgattcag acgggcgctg
     3841 atcgctttcc agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac
     3901 aaacgtgtaa cacgttctgc cttgatcggt gacagattat ctaacacgct gacggcccct
     3961 ttacgattat tgcagaccag gatcatatcg caacccgcat ccagtgatac ctgcccgcgt
     4021 tcggcataac tgcccataat cgcggcacct tccatcgata aatcgtcaga gaaaatcacg
     4081 ccgtcaaaac ccagttcctg acgcaaaacg gttttcagcc agtaggaaga accgctcgcc
     4141 ggacgcggat caacatcact gtagatcaca tgcgcaggca taatggcgtc gagtttattt
     4201 tcgcggatta acgtgctgaa aaccgacata tctttggcac gaatttccgc ttgcgggcgt
     4261 gggtcgcacg gcgtctcttt gtgtgaatct gcagttactg caccgtgtcc tgggaagtgt
     4321 ttcccggtcg ttttcattcc ggcttcatgc atcccatcaa taaaccgact ggcgattgcc
     4381 agggcttttt ctgggtcggc atgataagaa cgctcgccaa tcgccgcgct gatatgtcct
     4441 acatccagca ctggcgcaaa gctgatatca atatccatag cgatcatttc gctggccatc
     4501 agccaacccg cctcttgcgc cagtttgccg ccctcttcca ttcccaacag cgcagcaaag
     4561 gattgtgctg ccggtaagcg ggtaaaacct tcgcgaaaac gctggacgcg tccaccttcc
     4621 tgatctaccg ccaccaccag atgattgcgc gatgctgcgc ggatctggcg caccagttca
     4681 cgcaactggg caggatcatg atagtttcgc gtaaagagaa tcagccctcc caccagcgga
     4741 tgcgccagta tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc caacattact
     4801 ggacccacac tgctctcctt attgttttat taatagctgc cgccaggtgt catcggccag
     4861 cctgataaat tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc ctgctttgag
     4921 catcagcagc cagggaaacc atcgcctgac ctgacgccat aattgcgccg gataaatctt
     4981 cgcgcgagtg gcatagtcat tgaccaattg ccggtgctgt tcagtatttt ccacccacac
     5041 cgccgccagt tccagcgcga tatcaccatc tcctgcatac tcccagtcga tgagttttaa
     5101 ccctgacgcg ctatgcacta aatttccggc gtggacatcc atatgcaatg gacttaagcg
     5161 taaaggccgt ggttcccgcg ctttgcgcag acgttttaac attcgcagcc aacccactgt
     5221 ccgccgcgcc ggatcgcttt gctgccagta cagttccagt aacggcaaca gcgttattcg
     5281 ccagccaaaa cgtggttgtt gatgtagata atacagcaag cctgccagtt cgttggtatc
     5341 cggcaaatac gtttttacct cgccgggcag atagtcgact accatccagt cacggagata
     5401 taaatgcggc ttcggtgcaa tgcatgcggg tagttgtgat aaagcccgat actggcgcaa
     5461 gaacgcggac tgcggcgcat caggatcgtg cggctgacgc acaacaaaac gctggccctg
     5521 atgttcaatg agaaaactac cgccactaag cccactatta aacgtcgtga cgggatgaaa
     5581 ctgcgggaaa aagcgcgaca gcaattcgtc gcgcgtgatg ggattattgc tgcgaaacgg
     5641 cacctttacc tgaccagata atttcgcccg tctgcaccag catcagctgc atttgtaggg
     5701 tcggagcgtt aacgttgcca gaggcgctgg agtacagcac gtaatgagcg ccgacattgc
     5761 gggcaatgcc tatggcttta ctacgggtgc ctaaactatc ctgcggcgac aaacctaact
     5821 gttgcttagc catcgacagc tgctgggcgg aaaccagggt aaatttcccg ttattagcca
     5881 gcgcatttcg cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta gtacggttgt
     5941 taacgctatc aaccagcagg acgctacccg cagtcacccc gtcagcccca agcatcttac
     6001 tgaccatcgg ctgcattgcg ccattccagt cataatggcg aatatgcggc gcaggcggtg
     6061 cagtttgatc ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc accgagggca
     6121 ctgtggggac aggctgttgt ggctcggctg gttgttccgg cgctggtttc acttcttcta
     6181 ccggtgcagg ttcacgttgc cccacacacc cggcgagaaa catcgccagc gcggtaatca
     6241 aggcgtagcg actcattttt gtcatcaaga ttcacccctt acaaatatag ataaagtctg
     6301 actttgtgcg cccccagaaa attggcgctg ccgtacagcg ttaccgccga atgtgcggga
     6361 atggtaacgc tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag
     6421 taaaaacgat aatgaacggt aacgggttct tgcctttcgt tataaagcgt tgaggatgct
     6481 gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat
     6541 aaagatgact ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga
     6601 cagcccacca gcagtaacaa cgccagagac accagcccaa agcatccttt tctcatcgtt
     6661 acagaccttt atgcgccagc attggtccca gcggacggcc acccaacaag tgcatatgga
     6721 tgtggtaaac ctcttgtccg ccatggcggt tggtgttcat gatcagacga tagccatctt
     6781 cggcaatacc ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct
     6841 gctcatgctc agctgagacg tcgttcacag tcggaatgag gatattcgga atgatcagaa
     6901 tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga
     6961 cgatatcgga ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga
     7021 cattttcctt tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt
     7081 atagagtatg gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa
     7141 aaatcgtttt gctgccgtat atatctccat tattcccatt tctgcctgcg aatgcgctga
     7201 aaagacattc tctgtttggt ttacacgaca aatatgaatg cgtatatttc tcatttgcat
     7261 ttgcatttgc aagcaaaatt attcgcacac taaatataac cgctcatcac cctcctccgg
     7321 catgagcctg acgacaacat aaaccaagag atttcaaatg ctttcaacac aatttaacag
     7381 ggataatcaa tatcaagcca tcaccaaacc gtcactactt gccggttgca tagcactggc
     7441 actattacct tctgccgctt ttgctgcacc agccactgaa gaaacggtga ttgttgaggg
     7501 ttcagccaca gctccagatg atggcgaaaa tgattacagc gtaacgtcta cctctgcggg
     7561 taccaaaatg cagatgactc aacgtgatat tcctcagtcg gtcactattg ttagccagca
     7621 gcggatggaa gatcagcagt tacaaacgct gggcgaagtg atggaaaaca cgctggggat
     7681 cagcaaaagt caggcggatt ccgatcgtgc tctttattat tcccgcggat tccagatcga
     7741 taactatatg gttgatggta tccccaccta ttttgaatcg cgctggaatc tgggcgacgc
     7801 actttctgat atggcactgt ttgaacgcgt agaagtagtg cgtggcgcga caggactcat
     7861 gaccgggacg ggtaatccat ctgcggcaat taatatggtt cgaaaacacg cgaccagtcg
     7921 tgaatttaaa ggcgatgtct cggcggaata cggtagctgg aacaaagaac ggtatgtggc
     7981 ggatttacaa agcccactca ccgaagacgg taaaatccgc gcgcgaattg tcggcggcta
     8041 ccagaataac gactcatggc tggaccgcta caatagtgaa aagaccttct tctccggcat
     8101 tgtcgatgct gatttaggcg atcttacgac gctttcagcc ggttacgaat atcagcgcat
     8161 tgatgttaat agcccaacct ggggcggttt accgcgctgg aatactgatg gcagcagcaa
     8221 cagttacgat cgcgcacgca gtaccgcacc tgactgggcg tacaacgata aagagatcaa
     8281 caaggtcttt atgaccctga agcagcggtt tgctgatacc tggcaagcga cactgaatgc
     8341 cacccactct gaagtcgaat ttgacagcaa aatgatgtat gtcgatgcct atgtaaacaa
     8401 agcggatggt atgctggttg ggccatacag taattatgga cctggctttg attatgtcgg
     8461 cggcaccggt tggaacagtg gcaaacgtaa agttgatgcg ctggatttgt tcgctgacgg
     8521 tagttatgaa ttgtttggtc gtcagcacaa tctaatgttt ggtggcagtt acagcaaaca
     8581 aaacaatcgt tacttcagtt catgggccaa catcttcccg gatgaaattg gcagtttcta
     8641 caactttaat ggcaatttcc cacaaaccga ctggtcacca cagagcctgg cgcaggacga
     8701 taccacacat atgaaatcgt tatatgctgc cactcgtgtc acccttgccg atccgctgca
     8761 tctgatcctc ggcgcacgtt ataccaactg gcgggttgat acgctgactt acagcatgga
     8821 gaaaaaccac accacgcctt acgctggtct ggtgtttgac atcaatgaca actggtcgac
     8881 ctacgccagc tatacctcta ttttccagcc gcaaaatgat cgtgacagtt caggcaaata
     8941 tctggctcca atcaccggta acaactacga gctgggtctg aaatcggact ggatgaatag
     9001 ccgtctgacc accacgttag ccatcttccg tattgagcag gataatgtcg ctcagtccac
     9061 cggtacacct atccccggca gcaacggcga aaccgcctat aaagcggtgg atgggacagt
     9121 cagtaaaggg gtggaatttg aactcaacgg cgcaattacc gacaactggc agctgacatt
     9181 tggcgcaacg cgctatattg cagaggataa cgaaggaaac gccgttaatc ctaatctgcc
     9241 acgcaccacg gttaaaatgt tcaccagcta tcggttgcct gtcatgccag agttgacagt
     9301 cggcggtggt gttaactggc aaaatcgcgt gtataccgac accgtgacac cgtatggcac
     9361 cttccgcgcc gagcaaggta gctacgcgtt ggtggatctc ttcacccgct accaggtgac
     9421 gaaaaacttc tcgttacagg ggaacgtcaa taacctgttc gacaaaacct acgataccaa
     9481 cgtggaaggt tctatcgtct acggcgcacc gcgtaatttc agcattaccg gcacgtatca
     9541 attctgaata acacctgtaa aaaaggcagc catctggctg ccttagtctc cccaacgtct
     9601 tacggattag tggttacgga tgtactcatc catctcggtt ttcaggttat cggatttagt
     9661 accgaaaatc gcctgaacac cagaaccagc aaccactacg cccgctgcgc ccagtttctt
     9721 caggccagcc tgatccactt tagacacatc agcaacgctg acgcgcagac gggtaataca
     9781 tgcgtcgagg ttagtaatgt tttctttacc accaaatgca gcaaccagag ccggtgccat
     9841 ttcgctggta cctgtcgctt ttgcatcttc agtcgcgtct tcacgacccg gcgttttcag
     9901 atccagtgct ttaatcagca cgcggaagat ggtgtagtaa acaatcgcat aaccgatacc
     9961 gacgatcggg aacagccaca gtttgctgct gttaccagac agaacgatga agtcgatcag
    10021 accgtgcgaa aacgacgtac cgtcacgcat ccccagaaga atacagattg ggaatgccag
    10081 gcctgccaga atcgcgtgga tgatgtacag gatcggcgca acgaacatga aggagaactc
    10141 gatcggctcg gtgataccgg tcaggaacga ggtcagcgcc gcggagatca taataccgcc
    10201 cactttcgcg cggttttctg gtttagcaga gtgccagata gcaattgcgg cagctggcag
    10261 accgtacatt ttgaacagga agccaccaga cagtttaccc gcagtcgggt cacccgccat
    10321 ataacgcgga atgtcgccgt ggaaaacctg acctgctgcg ttggtgtatt caccaatctg
    10381 catctggaaa ggtacgttcc agatgtggtg cagaccaaac ggtaccaggc aacgttcgat
    10441 gaaaccgtaa atgccaaacg caactaccgg gttctggtaa gcagcccact gagagaaggt
    10501 ctggattgca gaaccaatcg gcggccaaat gaaggacagc acaacgccag taaagatggc
    10561 agccaggcca gaaatgatcg gcacaaagcg tttaccggca aagaagccaa gatactcagg
    10621 cagcttaata cggtagaaac ggttaaacat gtacgctgcg atcgcaccgg agataatccc
    10681 tccgagtacg ccagtatccg ccaggtgttt agaggcgatt tcttcagcag gtaaatgcag
    10741 taccagtggc gcaaccacgg ccatggtttt aaccatgatg ccataggcaa caactgcagc
    10801 cagcgcggat acgccatcgt tattggtaaa gccgagggcg acaccgatcg caaaaatcag
    10861 tggcatgttt gcaaagacgg aaccgcctgc ttctgccata acatgcgata caacggcggg
    10921 cagccagctg aaattcgcgg aaccgacgcc cagcagaata cctgcgatag gcagtacgga
    10981 taccggcagc atcagcgatt taccgacctt ttgcaggtta gcaaatgcat tcttaaacat
    11041 aattgagagt gctcctgagt atgggtgctt tttttacgtt ctcacgcgtg gcaagggggg
    11101 agagcctcgc cgtgtacagg gcatctaagc gccctttatt tattacacag agtaaaataa
    11161 ttcagtgcca atttgtttga cggctatcac gtttcaactt cagatcattg gagaagttgc
    11221 acattttgac aaaacatatg tcacaatatt tcaaacgtgg ttattcaccg cccaattttc
    11281 ggcggtgaac tttactcgtt ttagtcatta attacgagct ttaaaaaaac tcattcaacg
    11341 gatggattga aggcgggaag cgtcgatgtg aaacagacgg gcgaagttat cggtggttac
    11401 ctgcgccagt tcttcaacgg caacaccttt caacacagcc atgtattctg caacgtcacg
    11461 aaccatcgca ggttgattct cttttcctcg atgcggcacc ggcgcaaggt aaggtgagtc
    11521 agtttccacc agtaaccgat ccagggggac ataacgcgca gcatcgcgca gttgctccgc
    11581 attacggaag gtcacaatgc cggaaaagga gatgtaaaat ccgagatcca gtaatttgcc
    11641 cgccgtttct ctgtcctctg taaaacagtg tagtacgcca ccgcaatccg tcactttttc
    11701 ttcgcgcaga attgccagcg tatcggcgcg ggcgtcacgg gtatggacga taaccggctt
    11761 gttcagttca cgaccaatct ggatatgatg gatgaaggac tcttgctgac gtactttagt
    11821 ttccggtgtg taataataat ccagcccggt ttcacccagc gctacaacac cctcttctgc
    11881 cgccagacgg cgtaaatctt ctacatcgta gggatcattc tggttgagtg gatgcacgcc
    11941 acaagaaaat acgacgttgt cacgttcgcc taccagatcc cgcatgtgta aataacccgg
    12001 taatgttgtg gcgactgcca gacaaaattt cacatcgcgt gcggcggctt tcgccagaac
    12061 gtcatccacg tccttatgca aagattcata atccagacca tcgagatggc agtgtgagtc
    12121 gactaaaaac atgatgtctc tcttaaagat gaggaaccgg tagcacaacg cccggttgca
    12181 ggtaatgctc aatacgcagt aaaagagcgg tgatgagaag ctcgcggttg atgcctgtga
    12241 cagacattaa ctgttcacga atgtggcaaa catcccccag tatagcctgc aggcgcgagg
    12301 gagaaagatg gtttgccagt tcggcgacca ggcccggcac atcaacattg gtcacctgcg
    12361 cagcgccatg atggcgtttt agcgcatcca tcagcaacgt tgccagccag tgtaaacgcg
    12421 ccggagcttg ttcatgatta agggccgcca gcagcgaata ccagtcgccc gatggcacgc
    12481 tatatgccaa cgcctgacac aatgtttcac gagcctgcca gttatctccc tgaaacaacg
    12541 ccagtgccgc gccaggcgaa ccggcgctta agcgcaatgc ggcaagtaat gcatcctgtg
    12601 acattgtcac ttcgcgtgaa agccaggtca cggcgtactg ttccggcggc ggcgcaaggt
    12661 aatgtaaccg acaacgacta cgtaacgttg ccagtaaacg ttcaggctcg cgggtagcca
    12721 ggaaaaacca ggtttctgct ggtggctctt caagcgtttt cagcaatgcg ttagccgcgg
    12781 cgtcggtcag taaggcagca tcggttaccc agacaacttt cgcaccacct aagcgtgcgt
    12841 gctcattcag cttttcggtg acctcacgta ccgcatcaat gcccagcgta tttttccctt
    12901 tttcgggagc cagggtgtag taatcgggat gcgtgccagc ctgcatcaac tgacatccac
    12961 gacagtgacc gcaacttttg tggccctgcg gttgttggca gagtaaataa cggctcaggg
    13021 cgtagattaa agcatcatcg cccatgcccg gtaacgcctg aatgagtagc gcatggtgac
    13081 ctcttccggc ctgatagctg gctaccagtt tttcgaaatc aggtcgtaac catggatacc
    13141 atctcatgcg tccaactcct tcacccagcg ggtcacggta gtgcggattg catccatcac
    13201 ggcctccagc ggctgggtgg catcaatggt atgaatactt ttatcttgtg cagccagttc
    13261 cagatagcgg gcgcgggtgc gattaaagaa atcgaaagac tcttgctcaa tacgatccag
    13321 ctcgccgcgc gcacgcgcgc gttttaagcc aacttccggg gtaacatcga gatagagcgt
    13381 taagtcgggg cgaaaatccc cgagaacagc atcacgcagt gttgccagca tatgttggtc
    13441 aataccacgt cctccgccct gatacgcctg agtggagaga tcgtggcgat cgccaataac
    13501 ccaggtgccg ttagccagcg caggtttgat gaccgtttct accagttgaa cgcgcgcggc
    13561 ataaaacatc agaacttcgg ctttatcggt aatgatttca tcgcctaccg atttgatatc
    13621 cagcaccagg cttcttaact tttcggcaag ttgcgtacca ccaggttccc gagtgaaaac
    13681 catgtcgcgg atacccagtt gctcgagcgt ctcaaccacc acattacgcg cggtagtttt
    13741 gcctgcgcct tccagcccct caatgacgat atacttactg cgcatttttt tccttaagca
    13801 ctttcagata atcctgcaca gacttgttat gactggcaag attggtatta aacgtgtgac
    13861 cacctttacc atcggccaca aaatagagat acggcgtttt tgccggatgc gcagcagcct
    13921 tcagcgaatc cgcccccggc gtagctatcg cacccggcgg caaaccggta atggtatagg
    13981 tgttatacgc tgtcggcgtt tccaggtctg cacgagaaag tttgccatta taacgctctc
    14041 ccatcccgta aatcacggtt gggtcggtct gcaggcgcat accaatgcgt aaacggttga
    14101 taaataccga ggcaacctga tcgcgttcac tggcaatggc ggtttctttt tcgataattg
    14161 atgccatcgt caccagctga tttttatctt tataaggcag accgtccgca cgcccttccc
    14221 aggcgctatc gaccgctttc accattttct tgtgcgctcg cttgagtaac gcgacatcgg
    14281 tggtattggc ggtatacatc caggtgtctg gccagaacca accttcaatc cactccgggt
    14341 tttccagttc aagtgcctgc gctacggtga tgtacttatc atcgctcagc gtgtgcttga
    14401 tatacggggc ctcacgcaat tgcttgaggt agtcgctcag acgcatccct tctaccagtc
    14461 gcagagggaa ctgtgcttct ttaccgcttt ccaacaattt cagcatctcg cgcacggtca
    14521 tctgcggtgt aaagcggtaa gtcccggctt taaagtgaga aagatccggt tcgatacgca
    14581 gcaaccattg aaaaacccgt gggcgattga tgatcttatc ggcataaagc tgttcaccaa
    14641 gcgccagacg tccggtccct ggcttcaggg taaatatcgt ctcttctttg ataagcaatt
    14701 tgctgtcggc aagatagcga accttccaga cgcccacacc agcggcgata cccagtacca
    14761 ccagcaataa caagattatc aataacactt ttttcatgac taattcgggc gctcacaaag
    14821 tggggctaaa tattcatata acgttgctga cgaaaaggag acatcgccac aggcacgtac
    14881 gggcatcact ggcattaacg cattacaaat aaccatctca tctgcctgca acgcctcttc
    14941 cagagaggct tgcacttcga caagctgata agaggattgt gccagcaaac ggatacagaa
    15001 ttgtcgcata atgccgttaa cacctgcctg atccagtcgc ggcgtataaa ctacgttgcc
    15061 cttccgccag aacaaattag ctgcacagca ttccgtaacc cccccttcgc tgtcaaggac
    15121 cagcgcctca tcagcgtttg tctgctcaag atgagagcgg atcaatactt gctcaagccg
    15181 attaagatgt ttaatacctg caagatgagg attgcgcccc agccgcaccg ggcttagcgc
    15241 caacgtgatc ccttcgttac gcaaacggtc gtaatgtgca ggataagccg taacggagag
    15301 aatccgcgtt gccggtccac tgttcaatgt gctgtaccct cgcccgccac taccgcgact
    15361 gatcacgact ttcagtacac cattttgctg ttctgctgcc agcgttttca tctcttgttc
    15421 aagctgaggc cagaagtcac aggaaatcat caaccgctga caagcatcct gtagtcgctg
    15481 gatatgcgcc gataacaaac tgactttacc gtcgataact ctggcggtgg taaaacaacc
    15541 atcaccaaac tgcgttgccc gatcgcttac tgacagcgat tcctgcttat aaccgttaat
    15601 taagaacata ccggctcctt atcagagaat tgttagtgtg gcagcatgtt cactacggaa
    15661 caagtcggaa taaaagctaa gaaaaaaggc ccgcaagcgg accttttata tgggtggaaa
    15721 atgacaactt agatcttttt aaagatcaaa gaaccattag tgccaccgaa gccgaaggag
    15781 ttacacagcg tgtattccat tccgctgacc tgacgtgctt catgcggtac gaaatccaga
    15841 tcgcaacctt catccgggtt atccaggttg atggttggcg gaacagcctg atcgcgcagc
    15901 gccaggatgg agtagataga ttctactgca cccgccgcac ctaacaggtg accggtcata
    15961 gatttcgtgg agcttaccaa cacacggctt gcagcttcac cgaagatggt tttcaccgcc
    16021 tgcgcttcag ctttatcgcc agccggcgta gaagtaccgt gcgcgttaac gtagccaatc
    16081 tgactcgctt caatgcctgc atcacgcaga gcatttgcca ttgccagagc tgcgcctgcg
    16141 ccattttctg gcggtgacgt catatgataa gcatcgctgc tcataccaaa gccgacgagt
    16201 tcagcgtaaa ttttcgcacc gcgttttttc gcgtgttcgt actcttcaag taccagcata
    16261 ccggcaccat cgcccagtac gaaaccatca cgctctttat cccacgggcg gctcgccgct
    16321 tgcgggttat cattgcgggt agataatgca cgtgccgcgc caaaaccacc aacacccagc
    16381 ggcgtactgg ctttctctgc gccacctgca accatcacgt cagcatcgcc atacgcgata
    16441 atacgcgcag catggccaat gttgtgcacg ccggaagtac aggcggtcgc gatagagatg
    16501 cttgggccac gcaggccata catgatagtc agatgacctg ccaccatgtt cacaatcgtt
    16561 gacggaacga agaatgggct gatcttacgt ggaccaccgt tcatcagaga tgtgtggttt
    16621 tcttcgatca gtccgaggcc gccaatcccg gagccaattg cggcaccaat gcgggttgcg
    16681 ttctcttccg ttatttcaag gccagaatcc tgcatggcct gaacgccagc gacaattcca
    16741 tattgaatga aggcatccat cttgcgctgt tctttgcgcg agataatgtc ctcacagtta
    16801 aaatccttta ctaagccagc aaatttcgtt gcataggcgc tagtatcgaa atggtcgatt
    16861 aggctgatgc cactctgacc ggcaagcaga gctttccagg tagactctac ggtattgccg
    16921 acaggagaca acatgcccag tccggtcaca actacacgac gcttagacac gtttgtcctc
    16981 cagggaggga aaaaatgatt ctagtgggac aaaaagataa aactcaggcg gtcgaacgac
    17041 cgcctggaga tgttcactta cgcctggtgg ccgttgatgt aatcaatggc agcctgaacg
    17101 gtggtgattt tctcagcttc ttcgtccgga atctcagtat caaactcttc ttccagagcc
    17161 attaccagct caacggtgtc aagagaatcc gcgcctaggt cttcaacgaa agaagcattg
    17221 ttggtaactt cttcctgctt aacgcccagc tgttcgccga taattttctt aacgcgttct
    17281 tcgatagtgc tcatactctt aaatttccta tcaaaactcg ctttcgcgat ggttttcgta
    17341 gtgtataaaa tgttgaaaaa tttgcaacta aatcccggca ggtcttacca cgattttacg
    17401 ttattttgag gccttacccc ccaataacgc aaatcatttt gcactaatta ttactcataa
    17461 ccacgcagac tgcgcgcaac atgagcattt tgtgcaaatc gcggtcagac catgtacatc
    17521 ccgccgttca catgcaaagt ttcacccgtg atgtaagctg cttcgtcgga tgccaggaat
    17581 gcaaccgcgt tggcgatttc ctgtgcgccg ccgaggcgac ccgcaggaac ctgcaccagg
    17641 atacccgcac gctggtcatc gctcagcgca cgtgtcatgt ccgtttcaat aaagcccgga
    17701 gcaacaacgt ttacagtaat accgcgtgac gcaacttcgc gcgccagtga tttactgaag
    17761 ccgatcaagc ccgctttcgc cgcagcgtag ttggcctgac cgccatttcc catggtacca
    17821 accacagaac cgatagtgat aatacgacca tgacgctttt tcatcatagc gcgcattacc
    17881 gcttttgaca gacggaaaac agatgaaagg ttggtttcga taatatcgtt ccactcttca
    17941 tctttcattc gcattaacag gttatcacga gtgataccgg cattattgac caggatatcc
    18001 acttcaccaa attctgcgcg aattttttcc agaacagatt cgatagatgc cgggtcggtc
    18061 acattcaaca tcagaccttt gccgttagca cctaaataat cactgatcgc ctgagcgcca
    18121 ttttcactgg tcgcagtgcc aataactttc gcgccacggg ctgcgagcgt ttcagcaatt
    18181 gcgcggccaa ttccgcggct tgcaccggtt accagtgcga tttttccttc aaaattcatg
    18241 atgttcctct tttaaagctc gagcgccgct gccatcgctg aaggttcgtt cagcgccgag
    18301 gcggtcaggg tgtcgacaat gcgtttcgtc aggccagtaa gcactttgcc cgggccgact
    18361 tcatagagat gttctacgcc ttgcgctgcc atgtactcaa cagacttcgt ccactgaacc
    18421 gggttataca actgacgtac cagtgcgtca cgaatggcat caccattggt ttcgcatttc
    18481 acatcaacgt tattcacaac aggaactgtt ggtgcgttaa aggtgatttt cgctaattct
    18541 actgccagtt tgtcggctgc tggtttcatc agcgcacagt gagacggtac gctcactggt
    18601 aacggcagag cgcgttttgc gcccgcagct ttacaggcag cgccagcacg ctcaaccgct
    18661 tctttatgac cggcaataac cacctgtccc ggagagttaa agtttaccgg agaaacgacc
    18721 tgaccttctg cagcttcttc acacgctttt ccaatagacg catcatccag accgatgatt
    18781 gccgccatag cgcccgttcc ttccggtacg gcttcttgca tgaacttgcc gcgcatctca
    18841 accagacgca ccgcatcagc gaaatcaatc acaccagcgc aaaccagcgc ggagtattcc
    18901 cccaggctgt gaccggccat cattgccggt gctttaccgc catgctgctg ccatacgcgg
    18961 tacagcgcaa cagaggcagt caacagcgct ggctgggttt gccaggtttt attcagttct
    19021 tcagctggcc cctgctgggt cagcgcccac aggtcgtagc ccagcgccgc agaagcttca
    19081 gcaaacgttt cttcgacaat tggatagctc gccgccatat cagccagcat tccaacggtt
    19141 tgagaaccct gtccagggaa cacaaatgca aattgcgtca tgttttaatc cttatcctag
    19201 aaacgaacca gcgcggagcc ccaggtgaat ccaccgccaa aggcttcaag cagaaccaac
    19261 tgccccggct tgatgcgccc gtcgcgtaca gcttcatcca gcgcgcacgg gacagaggcc
    19321 gcagaggtat taccgtggcg atccagcgtc accacgacat tgtccataga cataccgagt
    19381 tttttcgccg ttgcactgat aatacgcagg ttagcctgat gcggaaccag ccagtccagt
    19441 tgagaacggt caagattatt cgccgccagc gtctcatcaa cgatgtgcgc cagttccgtt
    19501 accgcaacct tgaagacttc gttgcccgcc atcgtcagat gaattgaatt ctctggattc
    19561 acgcggtcgg cgtttggcag cgtcagcaat tcaccataac taccgtcggc atgcagatgg
    19621 gtggaaatga ttcccggctc ttcagaggca gccagcaccg cagcgcccgc gccatcgcca
    19681 aaaataataa tagtcccacg atctgttgga tcgcaggtgc gcgccagtac atcggaaccg
    19741 acgaccagag catacttcac cgccccagat ttcacgtatt gatcggctac gcttaatgca
    19801 taggtgaaac ctgcgcaggc tgctgcaacg tcaaatgccg ggcaaccttt aatgcccaac
    19861 atgctttgaa tctgacaagc tgcgctcggg aaagcgtgcg tagcagaagt cgttgccaca
    19921 acgatcaggc caatctggtc tttctcaatg cccgccatct caattgcgcg tgtcgccgct
    19981 tcaaagccca tggttgaaac ggtttcgttt ggcgcggcaa tgtggcgttc gcggataccg
    20041 gtacgagtga caatccactc gtcagaggtg tccaccattt tttccaaatc ggcgtttgtc
    20101 cgcacttgtt cgggcagata gctgccagta ccaataatct tcgtatacat gtacgctcag
    20161 tcacttttcg gttatatacc gtcacttgca aactgcgagt tcgctggcag cgtcctgcta
    20221 ccgcagagtt ccgcttttgc caccgtccag cagctcaaaa ccagctgggt atacagattc
    20281 caggcgagcg gcaattcgct gaggaacttg tcgctgcacc gcctgcactg cctgttcaat
    20341 cgcgaccgca aaagctcgct gattggctgc accatgactt tttatcaccg tgccgcgcaa
    20401 tcctaacaga caggcgccgt tatactggtc ggggttgagg tgactgaatc gcctcgtcag
    20461 gctcttttgt agccaacgct ttaataacag tagccaccac gaccgttttt tcccttcacc
    20521 ctgagatttc agcagagaaa ggaacatcct gacaacacct tccatcgtct ttaatgtgac
    20581 atttcctgta aagccgtcac aaaccagcac atctgtcttg ccagttaaca actcattggc
    20641 ttcaagatag ccgatataat tgatagaagg gattgtttta agcaccgctg aggcatcccg
    20701 aatactatcg agacccttta cttcttcttc accaatattg agcaacgcca cgcgaggatt
    20761 gggaatttcc accacctctt cagccaggac tgagcccata atggcaaatt gcaccaacat
    20821 tgtgctgtca caatcgacgt tggcccctaa gtcaaggacc accgttttgc ccttttgctg
    20881 atgtggtaat accgtcacca gcgccggacg ctcaatcccc tccaggggct tgagtaataa
    20941 ttttgccagc cccatcagtg ccccggtatt accggcactg acacaggctt gcgctcgacc
    21001 ttctttcacc agctccaggg ccacgcgcat tgaactccca cgactggcgc ggatagcttg
    21061 cgaaggccgg gcatcactgg cgataactga ctgcgcagga ataatctgca gacgcgaacg
    21121 ttgttcaaag tcagctttag caagtaatgg cgtgatggcg tcgggattgc cgactaaaag
    21181 aagagtgagt tgcgaattag aattcagtgc ctgcaatgct gcaggcactg tcacggaagg
    21241 gccaaaatcc cctcccatga catctaacgc cagggttaga cgtgtcaagg tatcgtcgcc
    21301 gcccggtttg gtctttcccc agttgcctgg ggaaaatcct cactaagctt catcacgcag
    21361 acgcgtgatt acttagcgat gaccttgcgg ccgcggtagt aaccgtcggc agtgatgtgg
    21421 tgacgcaggt gtttttcacc agaagttttg tctacagaca ggctggtgac tgcggtcagc
    21481 gcgtcatggg aacgacgcat gccacgtttg gaacgggttg gtttattctg ttgtacggcc
    21541 atggacctta ctcctcaatt acggtttatc cccgatccaa cggggagcac caattactta
    21601 cgctttaagc tggctaatac ggcaaatggg tttggctttt gcgcttcttc aggcagttca
    21661 ccaaagacca tgtccgcttc ggacacttca cagtgttcag aatcatgcac cggaactacc
    21721 ggcaaggcga ggatgatttc atcttcaacc attgcaagca gatcgatttc accgaattcg
    21781 ttaacctcaa tcggttcata cgcttccggc agtgcttcag cctgttcgtc tgaacgcaca
    21841 ggactaaaac aatacgttgt gtagacctga tgagtaaacg gcttcccgca acgctgacac
    21901 tcgagcgtta ccgtcacctt cgcatcgccg tttaacactg cgagacgttg gttatcgata
    21961 gcgaacgaca tggagcattc cacatcactg tccacactga ctacggattc ggcgacgcgc
    22021 tcaacctgat caggagtata gataccctgg taatcaaggc gtttttgagc cgtacgaacc
    22081 ggatcgagag tcaggggtaa ttttaccttt tgcatagggc gcgcatatta actttgtaac
    22141 gtcatagagt caaagaaaaa ggcagcctgt ggttgccttt tgccaataat tcgcacacat
    22201 tgcgggttac tgctttattt tcgttcagtg ctgataacga acatcaacct gcaaaacgaa
    22261 gcgcatagtt taaaatggct atcatcaata cgctatatct gatggaaaaa aatatgccta
    22321 aacttatttt agcctccacc tcgccctggc gtcgcgctct tctggaaaaa ctgcaaatct
    22381 cttttgaatg tgcagcaccc gaggtcgacg aaactccaca cagcgacgaa tcaccgcgac
    22441 agttggtgct tcgactggca caagaaaaag cgcaatctct ggcgtcacgt tatccggatc
    22501 atttaattat tggttcagac caggtatgtg ttcttgatgg cgaaatcacc ggtaaaccgt
    22561 taacggaaga aaatgcccgt ctgcaattac gcaaagccag cggcaatatc gtcactttct
    22621 atactggact ggcgttgttt aattcggcga atgggcatct gcaaacagaa gtggagcctt
    22681 ttgacgtcca tttccgtcac ctgagcgagg cggagattga taattacgtg cgtaaagagc
    22741 atcccctgca ctgcgcgggt agctttaaga gtgaaggatt tggcattact ctgtttgagc
    22801 gtttagaggg gcgtgatcct aacacgctgg ttggtttacc gctaatcgcg ctgtgtcaga
    22861 tgttacgtcg ggaagggaaa aacccgctga tgggatagtt ttttgcgccg gatgcggtta
    22921 cgtcgcatcc ggcaatcggt gctggcggcc tgatgcggcg taaacgcctt atcccattat
    22981 catgcttata tgctcgtatt agcgcgcgtt acgcagcttt tgcaaacaac gcttcaaacc
    23041 atcatccatc ggcgcttcga tacgcatcac ctcaccggtc cccggatggg taaacttcaa
    23101 cgctgcggcg tgcaggaaca gacgatttaa tcccgtgcct gcttcagtga gctgtctgtc
    23161 aaattcacgg tcaccgtagc gatcgtcaaa ggcaatcgga tgacctgcat actgtgtatg
    23221 cacacggatc tgatgagtgc gccctgttac cggactacaa cgcaccaggg tggcaaatgc
    23281 atagcgttct tccactttaa agcgtgtttc cgacggtttg ccttcctgac tcacacgcac
    23341 gatacgttcg ccgctttgca gaatattttt caataacggc gcctgaacgc tcttcacatg
    23401 cgactgccac tgaccgcgca ccagcgccag gtaatctttt tgcatccctt tttcacgtaa
    23461 ttgctcatgc agagaacgca acgccgagcg ttttttcgct accagcaaca cacctgaggt
    23521 gtcccggtca agacgatgaa ccagttcaag gaaccgcgct tccgggcgca acgcccgcaa
    23581 accttcaata acgccgaagc ttaaaccact gccgccatgt accgccgtac cggaaggttt
    23641 attcagcacc aggatgtgat catcttcata taagatgacg tccgccagcg ccgccacctt
    23701 ttgcagatgt ggcgaaaccg cctcttcttc ccgctcagca acgcgaaccg gtggaatacg
    23761 cacctcatca cccgcttcga gtttatattc aggcttaata cgttttttgt tcacccgcac
    23821 ttcgccttta cgcaaaatac ggtaaatcat actttttggt acgcctttca attgggtacg
    23881 caaaaagttg tcgatacgtt gccccgcttc gtcggcggtg atagcaacaa tttttacgga
    23941 tggagtctct gttttcatgg tgggcgattc taattagcca acaggattcg cgccactcat
    24001 ttttctatgc ttatatttag ttttgcacct tattacttca ctacgtgatc actttattga
    24061 tggttattaa accaatcacc agcaagaagt gaaaaaactg tgagtaagcg ggtgataaat
    24121 ggtaaaagtc atcttgctat aacaaggctt gcagtggaat aatgaggccg tttccgtgtc
    24181 catccttgtt aaaacaagaa attttacgga ataacccatt ttgcccgacc gatcatccac
    24241 gcagcaatgg cgtaagacgt attgatcttt caggcagtta gcgggctgcg ggttgcagtc
    24301 cttaccggta gatggaaata tttctggaga gtaataccca gtctgtttct ttgataattg
    24361 cgctgttttt ccgcatgaaa aacgggcaac cgacactctg cgcctctttg agctgacgat
    24421 aaccgtgagg ttggcgacgc gactagacac gaggccatcg gttcacaccc ggaaaggcgt
    24481 tactttgccc gcagcttagt cgtcaatgta agaataatga gtaagttacg atgaaaagaa
    24541 tgttaatcaa cgcaactcag caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc
    24601 tgtatgacct ggatatcgaa agtccagggc acgagcagaa aaaggcaaac atctacaaag
    24661 gtaaaatcac ccgcattgaa ccgagtctgg aagctgcttt tgttgattac ggcgctgaac
    24721 gtcacggttt cctcccacta aaagaaattg cccgcgaata tttccctgct aactacagtg
    24781 ctcatggtcg tcccaacatt aaagatgtgt tgcgtgaagg tcaggaagtc attgttcaga
    24841 tcgataaaga agagcgcggc aacaaaggcg cggcattaac cacctttatc agtctggcgg
    24901 gtagctatct ggttctgatg ccgaacaacc cgcgcgcggg tggcatttct cgccgtatcg
    24961 aaggcgacga ccgtaccgaa ttaaaagaag cactggcaag ccttgaactg ccggaaggca
    25021 tggggcttat cgtgcgcacc gctggcgtcg gcaaatctgc tgaggcgctg caatgggatt
    25081 taagcttccg tctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc cgcccggccc
    25141 cgttcctgat tcatcaggag agcaacgtaa tcgttcgcgc attccgcgat tacttacgtc
    25201 aggacatcgg cgaaatcctt atcgataacc cgaaagtgct cgaactggca cgtcagcata
    25261 tcgctgcatt aggtcgcccg gatttcagca gcaaaatcaa actgtacacc ggcgagatcc
    25321 cgctgttcag ccactaccag atcgagtcgc agatcgagtc cgccttccag cgtgaagtcc
    25381 gtctgccatc cggtggttcg attgttatcg acagcaccga agcgttaacg gccatcgaca
    25441 tcaactccgc acgcgcgacc cgcggcggcg atatcgaaga aaccgcgttt aacactaacc
    25501 tcgaagctgc cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc ggcctgattg
    25561 ttatcgactt catcgacatg acgccagtac gccaccagcg tgcggtagaa aaccgtctgc
    25621 gtgaagcggt gcgtcaggac cgtgcgcgta ttcaaatcag ccatatttct cgctttggcc
    25681 tgctggaaat gtcccgccag cgcctgagcc catcactggg tgaatccagt catcacgttt
    25741 gtccgcgttg ttctggtact ggcaccgtgc gtgacaacga atcgctgtcg ctctctattc
    25801 tgcgtctgat cgaagaagaa gcgctgaaag agaacaccca ggaagttcac gccattgttc
    25861 ctgtgccaat cgcttcttac ctgctgaatg aaaaacgttc tgcggtaaat gccattgaaa
    25921 ctcgtcagga cggtgtgcgc tgtgtaattg tgccaaacga tcagatggaa accccgcact
    25981 accacgtgct gcgcgtgcgt aaaggggaag aaaccccaac cttaagctac atgctgccga
    26041 agctgcatga agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa cgtaagcgtc
    26101 cggaacaacc tgcgctggca acctttgcca tgccggatgt gccgccagcg ccaacgccag
    26161 ctgaacctgc cgcgcctgtt gtagccccag caccgaaagc tgcaccggca acaccagcaa
    26221 ctcctgcaca acctgggctg ttgagccgct tcttcggcgc actgaaagcg ctgttcagcg
    26281 gtggtgaaga aaccaaaccg accgagcaac cagcaccgaa agcagaagcg aaaccggaac
    26341 gtcaacagga tcgtcgcaag cctcgtcaga acaaccgccg tgaccgtaat gagcgccgcg
    26401 acacccgtag tgaacgtact gaaggcagcg ataatcgcga agaaaaccgt cgtaatcgtc
    26461 gccaagcaca gcagcagact gccgagacgc gtgagggccg tcagcaggct gaggtaacgg
    26521 aaaaagcgcg taccgccgac gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta
    26581 acgatgataa acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa gagcaatctg
    26641 ttcaggaaac cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc
    26701 agctcaatca gaaagtgcgt tacgagcaaa gcgtagccga agaaacggta gtcgcaccgg
    26761 tagctgaaga aactgtcgct gccgaaccaa ttgttcagga agcgccagct ccacgcacag
    26821 aactggtgaa agtcccgctg ccagtcgtag cgcaaactgc accagaacag caagaagaga
    26881 acaatgctga taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc
    26941 acctgcgcgt gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat ccaacccaat
    27001 cgccaatgcc gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc aaagtctgga
    27061 tccgctatcc gattgtacgt ccacaagatg tacaggttga agagcagcgc gaacaggaag
    27121 aagtgcaagt gcagccgatg gtgactgagg tccctgtcgc cgccgctgtc gaaccggttg
    27181 ttagcgcgcc agttgttgaa gaaatggctg aagtcgtaga agcccccgta ccggttgccg
    27241 aaccgcaacc ggaagtggtt gaaacgacgc atcctgaagt gattgccgcc gcggtaactg
    27301 aacagccgca ggtgattacc gagtctgatg ttgccgtagc ccaggaagtt gcagaacacg
    27361 cagaaccggt ggttgaaccg caggaagaga cggcagatat tgaagaagtt gccgaaactg
    27421 ctgaggttgt ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtcgtcgctg
    27481 aagtcgcagc agaagttgaa acggtaaccg cggttaaacc agagatcacc gttgagcata
    27541 accacgctac cgcgccaatg acgcgcgctc cggcaccgga atatgttccg gaggcaccgc
    27601 gtcacagtga ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc
    27661 atacggcaac gcatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat
    27721 tagctcaagt aatcaagccc tggtaactgc cagggctttt ttatttcatc tttgaatcaa
    27781 catattaaaa ttcatcgaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa
    27841 tgcccgcccc ggcgatccct gagcgggcag acaaaacata cccagtttca tgatatgttt
    27901 taaagcgagc ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt
    27961 tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt
    28021 ccacatcaac cagatcgctc atctgctgcg tttgccctaa tgcgcgatcg ctacctaatg
    28081 aatccagcga ctccagttcg ttcagctgcg tgcctaattc cgcgcgaacg gtcaggacat
    28141 tgttcagcga gtttttcagt ccgcggttgg ttttatctaa cgccgcagcg gcggtttctt
    28201 tatccgcttc gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg
    28261 caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac
    28321 tgtcgaaaat tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt
    28381 gcttaatact ttctgttccg ccgacataat caccgttaac ctcactaaac ggcgcagtct
    28441 ctgttttata accggcaaaa atgtagcgcc cgttaccgtc agtggtgttc gccagattca
    28501 gcaactggtc acgaagcccc tgaatatccg tagccagcga ggcccggtca ttgtcactca
    28561 aggtgccatt gctggcgtag acaatttttt cctgagcatt ctggatagcg gtggtgacct
    28621 ggctaagtac actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt
    28681 actggctgtt ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat
    28741 cgtctgaagg gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt
    28801 ctgcctgaga attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac
    28861 gcattttatc tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg
    28921 cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc
    28981 atcgagattg acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc
    29041 ttgcgtggtg ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga
    29101 ggcataagcg tcgttaaagg atttcgctcc gcccaccgtt ttactgttgc tttgcagatc
    29161 cagcagggcc tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc
    29221 tattttcgct tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt
    29281 cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa
    29341 tgccacttta ccgttggcat ctggcgtcac cgtaaaagtg gtattgctgg caaggcgggt
    29401 gacctgccac tgattattat cgaacgagat tttgtaatct gtcgccagta ccgcggaggc
    29461 atcagttacc gtggcaccga tcgcaacgtt accgttgttt ttagtgtttt gcagaaccgc
    29521 gggcttaccg atagcaaaga aatcttcacc ctcatcgccg ttggcatcaa atccggcttt
    29581 gtgttggctg ttgaaagcct cagcaaatgc cagcgccagt tgtccaagcg tattacgcgt
    29641 ctggtccaga tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa
    29701 tttctccggg atctcaatat tgcctgccgt cccatcaaca taagcgacag tcgtacgaga
    29761 agggtcggcg ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga
    29821 gtaaccattg gccatcgtga tgttataagt gccgccatcc tgaacgctga cttctacacc
    29881 aacaatctgg tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga
    29941 cgcccctgcc cccacgcctg tcaggcggga gatttgatcg ttcaggctgg cgatttgttt
    30001 agcgtaattg ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg
    30061 gtcgcgaaga tactgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat
    30121 cagcgcctgg cgcgctgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa
    30181 gaaatcctgc atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt
    30241 cgacatctgc tcatagcggg cagtcaggcc gctactttgc gtctgcgccg cacgtaactg
    30301 gttggtaata aacgcatcat actcacgctg cacaccagaa acgtagacac cattaccaac
    30361 ccagccgcca gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata
    30421 tccggcaacg ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc
    30481 gttcagtccg ctcatggcgt tattgatcaa gctggacatg gaggttcctt ataagccttc
    30541 aattacagag tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca
    30601 atgttcatgc tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctgaatcatg
    30661 ttggtgagtt tacgggcata gtgaggatcg gtggcatagc ccgcgtcctg tagggcctgc
    30721 gccccctgct ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt acgtgttaac
    30781 agcccaacgt aatccgacaa tgcttccaga tacgagctgt agacccgaaa cttcgctttt
    30841 actttcttcg cttcgccatt ttcatattca gtcgtggtga tttcagtgac tggccctttc
    30901 cagttgccag aggctttgac accaaacagg ttatagctcg gctcgccgtt ttcgcggcgg
    30961 atttgccgtt gcccccagcc agattccagc gccgcctgag cgaggatcaa atgatgtggc
    31021 acaccgcttt gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgcttta
    31081 ctgtcacctg gcagcgaatc atcgtagtta cgtggcacgg ccttttgcac cagctgcgaa
    31141 agcgcctgat tttgataacg caccacggtt tcgagcggga atttcatcgg tgctgctggc
    31201 gtggactcct ctggcaatgg ttgttctggc gtcatctgct taaccatcat ctctgcaagc
    31261 cccagacctt tgcccgtcgt catctgttgg gcaatctgct ggtcatacat actggtatac
    31321 aggcgagtgt gctcgctgct gaacaggcca tcttttggta acgcgtcgcg catgcttttc
    31381 aacatcatct gcacgaacat cccttccacc tgacgggcca ccggacggat atttgccgcc
    31441 ggatcttcgc tcgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt
    31501 gccagtagtt tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg
    31561 cactttgcat tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga
    31621 gcgcgcgcac cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc
    31681 tctggcgtaa gtcgatctgc gtttgtggcg ttaccacggt ctgtccgcca ccaaacggtg
    31741 tatctggttg gctgacattg gcctgacgat tgactgttac tgagagattt ccctgcgcta
    31801 tcgcgcagct gtcgagagtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa
    31861 tcactacttt agcgtcctgc ggggtgacat taacctgcat attctggata tcggcaagga
    31921 aacggacctg ggaactgttg ccactcggta cgcgcacctg aatagtccgc gcatccaacg
    31981 cggtggcgct gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc
    32041 tgaaatcttc gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggta
    32101 attcacgttc aataactgca ccattggtga tccgtccacc gttcagttgg ttcacctgaa
    32161 cactgctacc gccagcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg
    32221 catacacctg actgtcaacg cccttaagcg gagtcatcaa caatgtgccg ccacgcaggc
    32281 ttttggcatt tcccatggaa gaaaccacca cgtcaatggt ttgcccctga cgtccaaacg
    32341 gtggaagtga cgccgtcacc attaccgcag caacgttttt tagctgcata ttggtgcccg
    32401 tcggaacggt aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg
    32461 tctgggttgt ctggtcaccg gtgccatcca gccccaccac cagaccatag ccaatcagtg
    32521 agttttgcct taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg
    32581 tcgtaaccag tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat
    32641 tggcgacagg ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta
    32701 gccattgcct acgtattcaa tgcgcgcatc cgccacctga gtagacggta cggtattgct
    32761 gccgctgata gtgcgtggat taaccacgcc agagaagcga ataaattcgg taccctgatt
    32821 aatggcaatc tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac
    32881 cgtcaccgtc aacgtgccgc taaaggtatt actggcattg gccccgcctt ttccgttgaa
    32941 cgtgttacca ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata
    33001 gcgcggcaca gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga
    33061 gcttttgctg gcgctaacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg
    33121 tggtcgacga tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa
    33181 aatagaaccg ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg
    33241 caccagcggc gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga
    33301 aatggcataa gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcaataaac
    33361 cggtgaacct gccaccggtt atgccttaga gttgcgtcag tttttgcagc atctgatcgg
    33421 tggtggacac cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca
    33481 gttcttccgc cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc
    33541 cgttcaggcc cggcgtgctt tcgttcggtg caccggagga ttgcgtttcg gtgtagaggt
    33601 tttcgccaat gctctccagc ccggtgtcat tcataaaggt ggtgagattg agctgcccaa
    33661 cctgaaccgg agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg
    33721 tgatacttaa cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg
    33781 tcaccagctg cccgttctga tccacctgga aagagccgtc acgggtatag gctgatgagc
    33841 catctggcaa catcacctgg aaaaagccct gccctttaat cgcgacatct ttgctgttgt
    33901 tggtctgcga caggttgcct tggctgtgta agcgttcagt ggcgaccggg cgtacccccg
    33961 tgccgatttg taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctggc
    34021 gaatggtttg ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac
    34081 tgacgtttgc caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg
    34141 ttttggcgat ccataatgaa ctgatcataa aatgtcctgt agtggattgc tgacaagcag
    34201 agcagaagca cgcggcggtg cgaatttccc ctcaccctaa ccctctcccc aaaggggcga
    34261 gggaacagtc cgctcgcttt tattgcaatg tcatcaatcg ttcaattaac tcatcgacag
    34321 cagttggttg gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc
    34381 aaaacgccgc gcgctggcaa tcatgtcgct cattgccgca acggcattga cgttactgcc
    34441 ttccagaacc cccgacatca cacgcaaggt tggatctgcc tgcagtaccg gtccacgcgt
    34501 ggcctgggtt tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca cttcgctgcc
    34561 cgtggctttc accagtttca gacgccccac tggcgcaacc gtatttgccg gatcgcccgg
    34621 attcagcgcc gagattgttc catcggcagc tatcgtgatt tccgcccctt ccggcacagc
    34681 aattggcccc gcctcgccta tcaccggatg cccctgaatt gtcagttgcc cggtgggatc
    34741 aacctgaatg ctgccattac gcgtataccc ttcgctgccg tcagcggtct gcacggccaa
    34801 ccagccatcc tgctgcaacg caacgtccag cgggcgcgag gtgtaatcca tcttgccggg
    34861 cgttatgtct gcacccggtg ttgacgccgt taccagcgtg cgcgtgggca gtgaaagccc
    34921 ttccaccggc actgcgcgca aggcgtttaa ctgcgcgcga aaaccgggcg tagaggcatt
    34981 ggccagatta ctggcggtta ccgcctgttg attcagcgtc tggctggctg ctcccatcgc
    35041 ggtataaatt gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag ttaaccagcg
    35101 tgttgaggat ctggtcctgg gttttgatgg tctgggcgtt agactgatag ttacgctggg
    35161 caacgatcat attgaccagt tctttactga gatcgacgtt ggacgcttcc aacgcaccgt
    35221 tggtcagggt gccaaagttg cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt
    35281 gcgtcgcaga ccagacgttg tcgccttcgg atgccagacc ttcgttgttg gcaaagttcg
    35341 ccagtacaat ctgccccagc agttgggttt gttcgttgga atagttgccg acaaccgtac
    35401 cgtcatcatt gatttgataa ctcaccagat cgcccggttt gtagccattc tgggtggttg
    35461 ccacaatgtt gttagcgccg gtattttgct gcatggagtt gaggaagctc aggctaaacg
    35521 tggcgggttc tgcgccgtta attgcgccgg tggtaatatt ctctgttgga tttgaggtca
    35581 gaacgccatt ggcattaaac accagcttca ttgcaggctc ggctgtacct gttggatcac
    35641 tgctatcctg ggtatagacc tgccagttat tatccccggt cttcacaaag tagacgctca
    35701 tgtcatgagc attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat
    35761 ccgcattgct ggcatcaaat gcgttaacag agggaagcgg atcgctggaa ttcaggttga
    35821 tctgcatcga cgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatgt
    35881 tagtcggatt cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca
    35941 gctgtaaacc ttgcatattc accaggttac gattttcatc cagcttaaat tgtccgttac
    36001 ggctgtagaa caccgaaccg ttgctatcta ccagacggaa aaaaccgttc tggctgatag
    36061 caacgtccag accacgcccg gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac
    36121 cggcaacttt tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg
    36181 tgcctgattt aaagccgtag gtggcggagt tggcgatatt gttgccaata acatcgaggt
    36241 tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa
    36301 atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga
    36361 gatccagcgt attaccgttg ttgccgcgga tcacaccctg caccagagca aactgcagcg
    36421 gctgggcaac cagttgtgta ccaccgttac tggcgctaat cgctacgttg taagaaccgt
    36481 tcggcgcagt gctgccgtca gtcaacgtac cgtcccaggt gaaactgtga actccggcgg
    36541 tcagttcacc aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg
    36601 taactttgtc tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc
    36661 cttcttcact gccggttccc gcaagaacag tggtgccggg gatcatcacg ccgtgaccga
    36721 tcaggttact ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagcg
    36781 tggtattgag tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct
    36841 cgttgttttc cattggattg gtcgggtcct ggtttttcag ctgcgccacc aacaaagtca
    36901 gaaaactgct ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac
    36961 tgacgccggt atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta
    37021 ttgaccgagc gtaagggttt tcagcatcat gcttttcacc gtgttgagca cttcaacatt
    37081 ggcctgatag ctgcgtgacg ccgacatggt gttaaccatc tctccgacaa catcaacgtt
    37141 cggcattttt acgtagccct ttgcatccgc cagcggatta cccggttcat aaaccagttt
    37201 gtccggggcc tgactttcta taacatcggc aacctttacg ccgcctgtcg cagcacctgg
    37261 tgcagcgtta acctggaata ccacctgttt tgcccgatat ggctgtccat caggaccggt
    37321 cacgctatca gcattcgcca gattactggc cgccacgttc aggcgctggg actgggcagt
    37381 taacgccgac ccggcgatat caaaaatatt cagcagtgcc atccgttaat ttccgctctg
    37441 taaaacgttc atcatgcctt tgatttgccc gctcaacgcg ctaaggctca tctggtattg
    37501 caggctgtta tcggcaaact gggtgcgttc gcgatccata tcgacggtat taccgtcaag
    37561 cgaaggctgg tccggaatac ggtattgcag ttctgcggta ggaggcgtca gcgcctgcgc
    37621 cggaatgtgt tgcgttgagg tcatcgtcag agcaaccaca ctggttgcat tccgtccacg
    37681 ttgcatgact tttttaagtt cactggcaaa atcgatatcg cgcgcctgat aaccaggggt
    37741 atcggcattg gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc
    37801 ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc atatctcctc cgcaggtatc
    37861 aaaattctgc catcagctta aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa
    37921 atgggtcgct atttatgccg ttgatggtca ttgtggacag gtacaattca cgttgtagaa
    37981 atggctgggg gcgaaaatgc tggcaataaa acgtagcgtg gcgatcatcg cgatactgtt
    38041 cagtccgtta agtgcggcga gcaatctcac atcgcaattg caaacctttt ttagctccca
    38101 gctcgcaggg ataagtgatg aggttcgtgt ttctattcgt acagcgccca atctactacc
    38161 gccgtgcgag cagccattgc tttcgatgag caataattcc cgcttgtggg gcaatgtgaa
    38221 tgtgttggca cgctgcggta acgacaaacg atatttacag gttaatgtgc aggccacagg
    38281 aaattatgtg gttgccgcga tgcccattgt gcgaggagga aagctgggag ctggcaatgt
    38341 caaactgaaa cgcggacggc tggatacctt gccaccgcgt acggtgctgg atatcaatca
    38401 acttgttgat gccgttagcc tgcgcgatct atcacccgat caacctatcc agttaaccca
    38461 gtttcgccag gcatggcggg taaaagcggg acaacgcgtc aacgtgatcg ccagcggtga
    38521 tgggtttagc gccaacgcag aaggtcaggc gctaaacaat gcagccgtcg cacagaatgc
    38581 gcgggtgcgc atggtatctg gacaggtagt cagcggcgtt gttgatgcag atgggaatat
    38641 tcttataaac ctgtaagctg ttaaagatta cccgtccctt gccgataaat aagcaacaca
    38701 tgataaaagc gccctcaatg aggaataaac catgagtatt gatcgcactt cgcctctgaa
    38761 gcccgtaagc accgttcaac cgcgcgaaac cactgacgcg ccggtaacga acacccgagc
    38821 ggcaaaaaca acagcctcca ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact
    38881 gatgcaaccc ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa aactggcgat
    38941 tcgtaacggt gaactaaaaa tggacaccgg caaaattgcc gatgcgctga tcaacgaagc
    39001 gcagcaagac ttgcagagta attaatcgta tgacgcgtct tgcagagatc ctcgaccaga
    39061 tgtccgctgt gcttaacgat ctcaaaacgg taatggatca agagcagcaa catctctcta
    39121 tggggcagat caacggcagc cagttgcaat ggattacaga acaaaaaagc tcactgctgg
    39181 caacgttgga ttacctcgaa cagttacgca ggaaagaacc caatacagca aatagcgttg
    39241 atattagtca acgctggcag gaaattgctg tgaaaacgca gcaactacgc caaatgaatc
    39301 aacataacgg ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt
    39361 tgaaaccgca tcaggaaccg acgctatatg gggcgaacgg tcagacctca acaacccatc
    39421 gcggcagtaa aaagatttcg atctgaagat tatctccggc ctgcactgca ggccggaatg
    39481 cattgttaca ccgtccggcg ggcaaattct ttaactttga agcccagtac cgccagtgca
    39541 gcgaagtacg cggcaatccc cgccagcacg accgccatta aacgcagcaa acgccagggc
    39601 atggtaccca atgaccactc cggcatgata tgtaacatac ctaaaagcac gccagacatc
    39661 accagtaccg ccaccaccag acgcaacaga aacgccatcc agccaggttg cggggtaaag
    39721 attttctgct tacgcaactg ccagtaaagc agcgaagcat tcagacacgc cgccagacca
    39781 atagaaagtg acagcccggc atgtttcaac ggaccgataa acgccaggtt catcaattgc
    39841 gttaaaatca gcgtaacgat ggcaattttc actggcgttt taatgtcctg gcgggaataa
    39901 aagccaggag ccaacacttt cactacaatc aggccaatca aacccaccga gtaggcaatt
    39961 aacgcccgct gggtcatcag cgcatcaaag gcggtaaatt taccgtactg gaacagcgaa
    40021 acggttaacg gaccggaaag aatgcccaac gcaaccgcac tcggcaacgc caacaggaaa
    40081 caaagacgca gtccccagtc catcaaacgg ttgtattcat catgattgcc actggcaaaa
    40141 cttttcgaca gcgacggtag caaaatggtg ccaagcgcca cgcccagcac accggacgga
    40201 aactccatta agcggtcggc gtaatacatc catgacaccg aaccggaagc aagaaacgag
    40261 gcaaaaatgg tgttgatgat taaggagatc tggctcacag agacgccaag gattgccggt
    40321 cccatctgtt tcaccacacg cattgctccg gcatcgtgga agttaatgcg tggcaaaacc
    40381 agcatgccga tcttcttcag atgcggtagc tgatacacca gctgcaggac gccgccgacc
    40441 gtcacagccc acgccagcgc cagcaccggt gggttaaagt acggtgcggc aaacagcgcg
    40501 aaaccaatca tgctgatgtt aagcagtgtt ggagcaaacg ccggaatcga gaagcggttc
    40561 cacgtattca gaatcgctcc caccagcgac gccagggaga tcagcaagat atagggaaag
    40621 gtaatcttta gtagctggct ggtcagggca aatttgtcag ctgtgtcagc gaagcctggc
    40681 gcggtcacca tgatcaccca cggtgcggcg agcatgccag cgaccgtcac aaccgccagc
    40741 gcaagtgtca gcaggccaga aacataagag acaaagaccc gcgtggcgtc ttcaccctgc
    40801 ttacttttat attccgccag aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag
    40861 atacggcgta acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct
    40921 gcgccaaaga ttctggcgac aattgcgtct cgtgcgaagc caagcacacg cgaaaacatg
    40981 gtcatcgagc tgacggcggc cagcgatttt aataaattca tcggtgttct aatccagacc
    41041 cacgggcaaa acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac ctggattatg
    41101 gtgaattgct accgccagat gttacagggt ttattcactc atcgcatcgc gccagatctt
    41161 gtcaacgata cgttgcgcca gcactgcctg ttcgccggcg gtttgcggaa ctgtctgatt
    41221 ttgcacgcat tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct caagcgtgct
    41281 ctgccaacca ggaatcggtt tattcactac accctgccca cgctcctcac gccattcgcg
    41341 catttccgta atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt cacgctgact
    41401 tccggcacgg cgatgcatac aagtggtgat ctgcaaagga ccggccgaaa aatggtgctc
    41461 ggcaaacagc atttcgccag cgtcgttagt cagtagcgta ccgccatcca gagaggcttt
    41521 gccgcccgac aaccacagcg cggtatccac cacatgcaga taatcatcca gcaacgtgaa
    41581 ataaagatcg tgtggcccga cgctattgct acgatgttta tccattctta gcgaggctgc
    41641 ggtggcgagt tgcgttttta actcacagta gagtggtgcg aaacgacggt taaaaccgac
    41701 catcaacgtc agttttttcc gcgccgccag ttccaccagc cgttcagcat cgcgcagatt
    41761 ttctgccagc ggtttatcga cacagacatg tacccccgca ttgagtaacg tactgaccac
    41821 gtcaaagtga ctggcggtgc tggaatgtac aaaaaccgca tcgcaactgg cggcaaggct
    41881 ggataacgaa tcggcataag gaatgcgcca gctttcacaa attggcaggg ctttcgcgcg
    41941 cgtaggcgac caggctcctt gtaacgtcca gtcagacgct gccgccagca ccggtaacca
    42001 cgctttttgc gcaatgccgc ctaatcccac tacgccgata cgtaattttt tcactttaat
    42061 ctcccaggtg ggccagcaac gaatcaagac gctgtttcag ttctgccact tcgatttcca
    42121 gggcttcgac gcgggcctgt aaatcaccgt caaccgcgtt cgacatatcc gttaccgccg
    42181 gctgatcctc tacctcacca ctgaaaagat gcatgtagcg gctttcgcgt ttacccggtt
    42241 cgcgggccag acgcaccaca aaaggaccat cttcgcgatt cgccagttgt tccagcgtca
    42301 actccacttc cgccatatcg ctgaattcat acattcgcgc ggcgcggctg cgcagttcac
    42361 ccggcgtctg ggcaccacgc aataacaacg tggtgattaa cgccacttct gcggcgctca
    42421 gtttcagatc gccaaattct gaattacaaa aacgttgctc atatttggtg acccgattac
    42481 caaaaccgct cactgtgcgt agataatgac gtttgaccag attatccagc tgttcctgca
    42541 cttcagattc actcagattc atgaccggtt cacggttcgt tttctgatta caggccgtga
    42601 ctacaccatt gactgagagc gggtattgct ccggcgtggt cacctgcttt tccagcaagc
    42661 aaccaatcac acgagcttcc agggcagtaa gttgatattt catgagattc tccttagcgg
    42721 ccgggcgtcc agtctggggt agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca
    42781 atcaacagat agtctttcgc atagccttct ttttcaaaac ccagtcgcgc cagtaaatca
    42841 ccgctgcgtt tattgtgcgg catataatta gccataatgc gatgaatatg ttgggtgcgc
    42901 tgcatataac gaatggctgc ggtcagggct tcaaacatga gtcctttgcc ctgccatttt
    42961 tgcccaatcg aataaccgag atagcaggca tgaaaagagc cgcgaacaac attggaaaaa
    43021 ttggcaacgc caataatctc tttttcgtcc gggtcgaata agccaaagta gaaagctgaa
    43081 ccttgtttat gaaattcgtt aatcatcccc agcctggcct gccagcctga tggataacag
    43141 tggctttcgt cgcacactgg ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa
    43201 tccgccagac gccaggcatc acgatcatgc accagacgca cgaccagtcg gtctgtggtt
    43261 aagcgcactt ttggcacgtt actgcgatag ccaaacattc tatacctact ccttcccgta
    43321 acgtctttaa tacgctgtaa gtattactat acctgcgcaa ccagaccctg tgaaaagtcc
    43381 aaaatgccat tttctgcatt attcacatat ttgataaaaa ctctctatca aagcgacgtt
    43441 ttgataaaaa aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag
    43501 aaagtgctca tcttttattt cccgggaggg gaaatgtccc gcgtgtcgca ggcgaggaac
    43561 ctgggtaaat atttcctgct catcgataat atgctggtcg tgctggggtt ctttgttgtc
    43621 ttcccgctga tctctatccg cttcgttgat caaatgggct gggccgccgt catggtcggt
    43681 attgccctcg gcctacgcca gtttattcag caaggtctgg gtattttcgg cggcgcaatt
    43741 gccgaccgct ttggtgccaa accgatgatt gttaccggta tgctgatgcg tgccgccgga
    43801 ttcgccacaa tgggtatcgc ccacgaaccg tggctattgt ggttttcatg cctgctctcg
    43861 ggactcggtg gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa actaatccgt
    43921 ccacaacagc gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc cggtgcggtc
    43981 attggcgcat tgttggggag ctggctgttg caatacgact ttcgcctggt ctgcgccaca
    44041 ggggccgttt tgtttgtgct gtgtgcagcg ttcaatgcgt ggttgttacc ggcatggaaa
    44101 ctctccaccg tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg cgacaagcgt
    44161 tttgtcacct atgtcctgac gctggcgggt tactacatgc tggctgtaca agtgatgctg
    44221 atgctgccaa ttatggtcaa cgatgtggct ggcgcgccct ctgccgttaa atggatgtat
    44281 gccattgaag cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg gagtgaaaag
    44341 cattttcgtc tggaacaccg gttgatggct gggctgttga taatgtcatt aagcatgatg
    44401 ccggtgggca tggtcagcgg cctgcaacaa cttttcaccc tgatttgtct gttttatatc
    44461 gggtcgatca ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc ggacgcaaga
    44521 gctcgcggca gctatatggg gtttagccgt ctgggtctgg cgattggcgg cgctattggt
    44581 tatatcggtg gcggctggct gtttgacctg ggcaaatcgg tgcaccagcc agagcttccg
    44641 tggatgatgc tgggcattat tggcatcttc actttccttg cgctgggttg gcagtttagc
    44701 cagaaacgcg ccgcgcgtcg tttgcttgaa cgcgacgcct gatttgatac tcccccctga
    44761 ctcctccata ctgaaagtga actgacagcc aaatggagga gtcacgtgaa gctatacatt
    44821 tacgatcact gcccttactg cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc
    44881 gtcgaattac atgttctgct caacgacgac gcagaaacac ccacccggat ggtcggtcaa
    44941 aaacaggttc ccattctgca aaaagatgac agccgctata tgccagaaag catggatatc
    45001 gttcactatg tcgataaact cgacggcaaa ccgttactga ccggcaaacg ttcccctgcc
    45061 attgaagagt ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt gccgcgtttt
    45121 gccaaatcgg catttgatga gttttctact cccgccgcgc gcaaatattt cgtcgacaag
    45181 aaagaggcca gcgcgggtaa ttttgccgac ctgctggccc actctgacgg tctgattaag
    45241 aatatcagcg atgatttacg tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat
    45301 ggcgaacttt cggaagatga tattcagcta ttcccgctac tgcgtaatct gacgctggta
    45361 gccggaatta actggccaag ccgcgttgct gattaccgcg ataatatggc gaaacagaca
    45421 caaatcaatt tgttatcatc aatggcgatt taatcctgac tcgccgcggc agcctgtatt
    45481 cgcgggttgc cgccgttttc agttttttct gctggcgagt ttatcgctgg tgacacatgc
    45541 tgcgttgtgt caggatattg acgcagattt catgtcgagg actaccatga acaagttttt
    45601 atttgccgct gcattgatcg tcagtggcct gctcgttggc tgtaatcaac tcacccaata
    45661 caccatcacc gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga
    45721 tattggttta cccggtgtgg ctgacgccca tattgttctg acaaacctga ccagccaaat
    45781 tggtcgcgaa gagccgaata aggttgccct aaccggagac gccaatctgg acatgaactc
    45841 cctgttcggt agccagaaag cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga
    45901 taaagaaaaa ggcgcgatct tcctgaaaga gatggaggtg gtcgatgcga cggtacaacc
    45961 ggaaaaaatg caaacggtga tgcaaacgtt gcttccctat ctgaaccagg cattacgcaa
    46021 ttactttaac cagcaacctg cttacgtcct gcgcgaagat ggcagccagg gcgaagcaat
    46081 ggcgaaaaaa ctggcgaaag gcattgaagt gaagccaggc gaaattgtca ttccatttac
    46141 tgattaatca cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg
    46201 cttatccttt gtgtccggca aaaacatccc ttcagccgga gcatagagat taatgactgc
    46261 accatcccag gtattaaaga tccgccgccc agacgactgg caccttcacc tccgcgatgg
    46321 cgacatgtta aaaactgtcg tgccgtatac cagcgaaatt tatggacggg ctatcgtaat
    46381 gcccaatctg gctccgcccg tgaccaccgt tgaggctgcc gtggcgtatc gccaacgtat
    46441 tcttcacgcc gtacctgccg ggcacgattt caccccattg atgacctgtt atttaacaga
    46501 ttcgctggat cctaatgagc tggagcgcgg atttaacgaa ggcgtgttca ccgctgcaaa
    46561 actttacccg gcaaacgcaa ccactaactc cagccacggc gtgacgtcag ttgacgcaat
    46621 catgccggta ttagagcgca tggaaaaaat cggtatgccg ctactggtgc atggtgaagt
    46681 gacacatgca gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa gcgtgatgga
    46741 acctctgcgc cagcgcctga ccgcgctgaa agtcgttttt gagcacatca ccaccaaaga
    46801 cgcagccgac tatgtccgtg acggaaatga acggctggct gctaccatca ctccgcaaca
    46861 tctgatgttt aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc tgtattgtct
    46921 acccatcctc aaacgcaata ttcaccaaca ggcattgcgt gaactagtcg ccagcggttt
    46981 taatcgagta ttcctcggta cggattctgc gccacatgca cgtcatcgca aagagagcag
    47041 ctgcggctgc gcgggctgct tcaacgcccc aaccgcgctg ggaagttacg ctaccgtctt
    47101 tgaagagatg aatgctctgc agcactttga agcattctgt tctgtaaacg gcccgcagtt
    47161 ctatgggtta ccggtcaacg acacattcat cgaactggta cgtgaagagc atcaggttgc
    47221 tgaaagcatc gcactgactg atgacacgct ggtgccattc ctcgccgggg aaacggtacg
    47281 ctggtctgtt aaacaataaa aatcgtagct tcctgttgtc attaggttat tttacctgta
    47341 taaataacca gtatattcaa cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa
    47401 cttctccatt gccagctggg gctattgacg ccctggctgg cgaactttcc cgccgtattc
    47461 agtatgcgtt tcctgataat gaaggccacg tatcagtacg ttatgccgca gcgaataatt
    47521 tatcggttat tggcgcgaca aaagaagata aacagcgcat tagcgaaatt ctccaggaaa
    47581 cgtgggaaag cgccgatgac tggtttgtca gcgaataata tgcagtgatt tttgtttttg
    47641 ccgggtcgcc ccggcttttt tattttactt gcgaattatg agtacaaaat ttctacactt
    47701 cctcaaaata attgcacaat atgctaaaaa aatatgaatg acatgctgtt ttattgttca
    47761 tataaatctt aaaagcagaa atttatctgt tccccccttt aaaaattcgg tgattaccct
    47821 tatttattgg tatactgaag ttgcttcaga taaaaacgca ttgaacctcg aataacgttg
    47881 tctagtaaca cgaattaggg ggccatgatg gaaaaaaata atgaagtcat tcagactcat
    47941 ccgctcgtag ggtgggacat cagcaccgtt gatagctatg atgcgctgat gttgcgtttg
    48001 cactaccaga ccccaaataa gtccgagcag gaagggactg aagttggtca gacgctctgg
    48061 ttaaccactg atgttgccag acaatttatt tcgatattag aagcaggaat cgccaaaatt
    48121 gaatccggtg atttcccggt aaacgagtat cggcgtcatt aacatgacat gaccatccac
    48181 ccaaaaggca cctcaccggt gcctttacca tttctgggtt gtatatctgc attcagttaa
    48241 ttactctgct taaaccatga gagcagagag aaaaaaatga aatacgatct catcattatt
    48301 ggcagcggtt ccgtaggcgc tgccgccggg tattatgcaa cccgcgccgg tttaaacgtg
    48361 cttatgaccg acgcccatat gccaccacat caacacggca gccaccacgg cgatacgcga
    48421 ttaattcgac atgcttatgg tgaaggcgaa aagtatgtcc cgctggtcct ccgcgcacaa
    48481 acgctgtggg atgacctctc ccgccacaac gaagatgatc ccatttttgt acgctctggt
    48541 gtcattaacc ttggcccggc tgactccgca tttctcgcca acgtcgccca cagcgccgaa
    48601 cagtggcaac tcaacgttga aaagctcgat gcgcaaggga ttatggcccg ctggccagaa
    48661 atacgcgtcc cggacaacta catcggctta tttgagactg attccggttt tttgcgcagc
    48721 gaactggcga ttaaaacctg gatccaactg gcgaaggaag cgggctgtgc gcaactgttc
    48781 aactgcccgg tcaccgcaat tcgtcatgac gatgatggcg taactattga aacggctgac
    48841 ggtgagtatc aggcgaaaaa agcgattgtc tgcgcgggaa catgggtaaa agacctgctc
    48901 ccggagctgc ctgtccagcc cgtacgtaaa gtatttgcct ggtatcaggc cgatggccgc
    48961 tatagcgtga agaataaatt cccggcgttt accggtgaac tgcccaatgg cgatcaatat
    49021 tatggttttc cggcagaaaa cgacgcgttg aagattggca aacataacgg aggccaggtt
    49081 atccattcag cggatgaacg tgttccgttt gcggaagtgg tcagcgatgg ttcggaagcc
    49141 ttcccgttct tgcgcaatgt attgccgggt atcggttgct gcctgtacgg cgctgcctgc
    49201 acctatgata attcgcctga cgaagatttt attatcgata ccctacccgg ccacgataat
    49261 acactgctca ttaccggcct gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa
    49321 atagctgccg attttgcgca agacaaaaaa agcgattttg atttgacgcc attcagcctt
    49381 tcccgcttcc aataatcatc attacggcct ccagaatgag gccgttttta caggagtatt
    49441 taatgcgtcg gctgttgcac tatctcatca ataatattcg cgagcatctg atgctatatc
    49501 tttttctctg gggattgttg gcaattatgg atctgatcta tgtgttttat ttttagtttt
    49561 ccgcactgac ataaactgac aaatatttaa tctccctttc atttagcgtg aaaaattgaa
    49621 tgagattatt cctgcttcta cagcactcac cagacatctc tgatgctgaa cccactaaac
    49681 ttatgaagat tctttcatta ttaattcaaa attattgaaa taaaaataaa tatcattcaa
    49741 tttgttattt ttgtgttgca attcattctc cgacacgcca ttttctttct ctcacaaatt
    49801 cgttttggat gcacttatgt cattcacaaa tacccctgaa cgctatggtg ttatatctgc
    49861 ggccttccat tggctaagcg caattattgt ctacggcatg tttgctttag gtctgtggat
    49921 ggtcacgctc agttattacg atggctggta tcacaaagca cccgaactac ataaaagcat
    49981 cggtattttg ttaatgatgg ggctggttat tcgcgttctg tggcgtgtca tatctccacc
    50041 gccgggtccg ctgccgagtt attcgcccat gactcgtctt gccgcaaaag ctggacatct
    50101 cgcattgtac ctattgctgt tcgccatcgg catcagcggc tatctgatct caactgccga
    50161 tggtaaaccg atcagcgttt ttggctggtt tgacgtcccc gcgacccttg ccgacgctgg
    50221 cgcacaggca gactttgccg gtgccctgca tttttggctc gcgtggagcg tcgtagtact
    50281 gtccgttatg cacggattta tggccctgaa gcatcatttc atcgataaag acgacactct
    50341 gaagcgcatg ctgggaaagt cgtcatctga ctatggagta taaaaaatga aaaaaagcct
    50401 gcttggttta accttcgcgt ccctgatgtt ctctgccggt tcagcggttg ccgccgatta
    50461 caaaattgac aaagaaggtc agcacgcctt tgttaacttt cgcatccagc atcttggcta
    50521 tagctggtta tatggcacct ttaaagattt cgacggtact tttacctttg atgaaaaaaa
    50581 tccagctgcc gataaagtga atgtgacaat taacaccacc agcgtcgata ctaatcacgc
    50641 cgaacgtgat aaacatcttc gcagtgcaga tttcctcaat accgcaaaat atccacaggc
    50701 aacattcacc tccaccagcg tgaagaaaga cggtgacgaa ctggatatta ccggcgatct
    50761 gacgctgaat ggtgtaacca aacccgtcac gctggaagcg aaattaattg gtcagggcga
    50821 cgacccatgg ggtggtaaac gtgcaggctt cgaggccgaa ggcaaaatta agctcaagga
    50881 cttcaatatc aagacagatt taggtccagc ttctcaggaa gtggatctga ttatttcagt
    50941 ggaaggcgta cagcagaagt aagagtgaaa tgttgccgga tggcacgcat ccggcaatga
    51001 tatttattct gttggatcag gaatgcccag tgttgtattc agacgtccac gagacttatt
    51061 aaagatctta ttgccatttt cacgtcctgc ccgacggcgt cgctgttctt ctggcggtaa
    51121 cgcgctttct tcgcagcaaa tttcactaca acaacctttg tatttttccg cacatactgg
    51181 acactgaata aacagcaggt ggcagccatc atttttacag ttggtatggc tgtcgcacgg
    51241 cgcaccgcac tggtggcaat gcgcgataat ctcatcagat atacgttcgc ccatccgctc
    51301 gtcaaaaaca aaatttttgc caataaaacg caccggcaag ccctgctcgc gcgccttacg
    51361 ggcgtattca ataattccac cctcgatatg ccagacttta ttgaatccgt tatgtttcat
    51421 ccaggcactg gccttttcac aacgaatgcc gccggtgcag tacatgacga tttttttatc
    51481 tttatgtgcc tgcatcatct cgactgcttt tggcagctgc tcacggaagg tatctgccgg
    51541 aatttccagc gcgttttcaa agtgccccac ttcatactca tagtggttac gcatgtcgat
    51601 aaacagtgca tcgggatcgt caagcatggc gttcacttcc gccgcttgca gatactcacc
    51661 aacattgctg gcatcaaagt gaggatcgtc aataccgtcg gcaacgatac gatcgcggac
    51721 cttcatgcgc agtacccaga aggatttccc gtcatcatcc aacgcgatat tcaggcgtaa
    51781 gccatctaaa gccgagtcga aggcatagag ctgcgcgcga aatgttttaa cattgctcgc
    51841 aggtacgctg atttgcgcgt taatgccctc atgcgccaga tacactcgcc caaaaacatt
    51901 cagcgcggta aacagctgat ataaagcgtc acgggtcgcc ttaggatcgg cgatgtggaa
    51961 atacttgtaa aacgaaatgg tggttcgcgg ttcgctctca gccaacattt tggcttttaa
    52021 cgcgtcgttg gaaatgcggt tgtgtaacac tggcatggtg tacggttcct gcgagatggg
    52081 aaagtaaaaa tccgcggcat gatatagcaa ttatcgataa ttaacatcca cacattttac
    52141 gctacatttg cgcattaaaa attatttgtt atttacaagc gcggcaattt cgcccagtct
    52201 tcagccacaa attttggttg cgggcgaaaa aatgcgacaa tacatacaat tgcccgaata
    52261 ggttgaaaaa caggattgat atgacgaatc tacccaagtt ctccaccgca ctgcttcatc
    52321 cgcgttattg gttaacctgg ttgggtattg gcgtactttg gttagtcgtg caattgccct
    52381 acccggttat ctaccgcctc ggttgtggat taggaaaact ggcgttacgt tttatgaaac
    52441 gacgcgcaaa aattgtgcat cgcaacctgg aactgtgctt cccggaaatg agcgaacaag
    52501 aacgccgtaa aatggtggtg aagaatttcg aatccgttgg catgggactg atggaaactg
    52561 gcatggcgtg gttctggccg gaccgccgaa tcgctcgctg gacggaagtg atcggcatgg
    52621 aacacattcg tgacgtgcag gcgcaaaaac gcggcatcct gttagttggc atccattttc
    52681 tgacactgga gctgggtgcg cggcagtttg gtatgcagga accggggatt ggcgtttacc
    52741 gcccgaacga taatccactg attgactggc tacaaacctg gggccgtttg cgctcaaata
    52801 aatcgatgct cgaccgcaaa gatttaaaag gcatgattaa agccctgaaa aaaggcgaag
    52861 tggtctggta cgcaccggat catgattacg gcccgcgctc aagcgttttc gtccctttat
    52921 ttgccgttga gcaggctgcg accacgaccg gaacctggat gctggcacgg atgtccggcg
    52981 catgtctggt gcccttcgtt ccacgccgta agccagatgg caaagggtat caattgatta
    53041 tgctgccgcc agagtgttct ccgccgctgg atgatgccga aactaccgcc gcgtggatga
    53101 acaaagtggt cgaaaaatgc atcatgatgg caccagagca gtatatgtgg ttacaccgtc
    53161 gctttaaaac acgcccggaa ggcgttcctt cacgctatta aatctcccat gccggatgct
    53221 tcagaatggc atccggcatt accacagcaa atccccctga tttagcgata aaagctctct
    53281 ggattgcgcc ccctggaagt cgggcgcata attagtgtgc ttatcttttc ttcttatgtt
    53341 caccgcgcct ggcgcaccaa cagcggattg ctatgtcacc ctgtgaaaat gacaccccta
    53401 taaactggaa acgaaacctg atcgtcgcct ggctaggctg ttttcttacc ggtgccgcct
    53461 tcagtctggt aatgcccttc taacccctct acgttgagca gcttggcgtt accggccact
    53521 ccgccctgaa tatgtggtcc ggtattgtct tcagcattac atttttattt tcggccatcg
    53581 cctcaccgtt ttggggtgga ctcgccgacc gtaaaggccg aaaactcatg ctattacgct
    53641 ctgctctcgg catgggcatc gtgatggtgt tgatggggct ggcacaaaat atctggcagt
    53701 ttttgatcct gcgggcgctt cttgggttac ttggcggatt tgtccccaac gctaatgctc
    53761 ttatcgccac acaagtaccg cgtaataaaa gcggctgggc actgggtacg ctctccacag
    53821 gcggcgttag cggtgcgttg ctcggcccaa tggctggcgg cctgctcgcc gatagctacg
    53881 gcttacgtcc ggtattcttt attaccgcca gtgtgctcat actctgcttt ttcgtcaccc
    53941 tgttttgcat cagagaaaaa ttccagccgg tcagcaaaaa agagatgctg cacatgcggg
    54001 aagtggtgac atcacttaaa aacccgaaac tggtactcag cctgtttgtc accacgttaa
    54061 tcatccaggt ggcgacgggc tcaattgccc ccattctgac gctgtatgtc cgcgaactgg
    54121 cgggtaacgt cagtaacgtc gcctttatca gtggcatgat cgcctcggtg ccaggcgtag
    54181 ctgctctgct gagtgcacca cgactcggca aacttggcga tcgaatcgga cccgaaaaga
    54241 tcctgattac ggcgctgatc ttttctgtac tgctgttgat cccaatgtct tacgttcaga
    54301 cgccattgca acttgggatt ttacgttttt tgctcggtgc cgccgatggt gcactactcc
    54361 ccgccgtaca gacactgttg gtttacaact cgagcaacca aatcgccgga cgtatcttca
    54421 gctataacca atcgtttcgt gatattggca acgttaccgg accattgatg ggagcagcga
    54481 tttcagcgaa ctacggtttc agagcggtat ttctcgtcac cgctggcgta gtgttattca
    54541 acgcagtcta ttcatggaac agtctacgtc gtcgtcgaat accccaggta tcgaactgat
    54601 ttttcgcctt tcatacttgc aaaagcggag aatcagctat ccttttccct gaaacctcat
    54661 caactcaaag ggagaatcgt gatgaccatg tacgcaacgc ttgaagaagc cattgacgct
    54721 gcacgcgaag aatttcttgc agacaacccc ggcatcgacg ccgaagatgc gaatgtgcaa
    54781 cagttcaatg cccaaaaata cgttttgcag gacggcgaca tcatgtggca agttgagttt
    54841 tttgccgacg aaggggaaga aggtgaatgt ttacctatgc ttagcggtga agccgcgcaa
    54901 agtgtttttg atggcgacta tgatgagata gagatccgcc aggagtggca ggaagagaat
    54961 acattacatg aatgggacga gggggaattt cagcttgagc caccgctgga taccgaggaa
    55021 ggacgcgcag cagctgatga gtgggatgaa cgttaattac tcatacgggc catgatgaat
    55081 gtcgatcggc agcagtaaag tatcaaaaac cagggagaat ggcagatcaa ggattgtgac
    55141 ataacgccag gcggagtcac gcacatccca ttgcacaccg ggataatatt ggttgccatg
    55201 cccctgcccc ggtatagtgc ggctaatgat gctgccacag ccactcagca aggtcaccat
    55261 gatgctcacc acaattaaac gcatttgata cctcttccgt aaaaaaatac cggcataacg
    55321 ccggtatttt tattggctcg ttgtttttgc cagatgtggc gtgaacgcct tatccgacct
    55381 acaaaatctt gcaaaatcaa cacattgcag gagcgatgta ggcctgataa gcatagcgca
    55441 tcaggcaact acgtttttat tgcgaagccg catccggttt acgcaatgcc agtggattga
    55501 gctttatccc ttcgtaataa ctcacccatg aagaatatct ctcaggggaa ttccagacgc
    55561 ggaaatgcag acgggccatc gtcaccggat cgcttagcag cacaaggcga cgatcgcgat
    55621 tcagcttctc tggcgtctcg ttcagcgcct gttcaacatg gcggtcacgg gcgatttcca
    55681 gcaccttact ggcgcggtga cgcgcggtcg ccattgcggt tgccagagcg ttaaatgacg
    55741 ggttaaacac tgcgtgcata aaaccatcat caagggagcg ttgacggttc atctcaagga
    55801 accgatcggt atcaaccagt acctgcggcg gtgaatactc ttccgggatc aggaacagtt
    55861 tccagcgttt ggtgcgcaga ccaacggtgg cacggctgga aatcaccgaa acaaacggtg
    55921 acaggatcaa cgagaagaca atcggtgcca gccagaacag gaaacgcaga tccaaccacg
    55981 ccatcccgac agcccacact aaccctaaca gcagctgtga gccgtggcgt ttgaacgctt
    56041 caccccagga agtggagtca tcatcacgct gcggtgaatt ccacaccact tcccagccaa
    56101 ggaacgcgct gacgacgaag accgtatgga acagcatgcg taccggagcc agcagcacgg
    56161 aaaacagcac ttccagcagc aacgataatg taacgcgcca gaagccgcca tattctttcg
    56221 ttcctttaca ccaaataagc aaaatgctca atagctttgg caggaacaac agcaccatgg
    56281 tcgaagcaaa aagtgcaatc gccagctcag gacgccactg cggccacacc gggaacaact
    56341 gccgtggttg caggaagtat tgcggttcgg tcaacgcatg cactacctgc aatgcagtag
    56401 agagcgcgag gaacataaac cacagcggag cggagagata agacatcacg cccgtcagga
    56461 ataccgcacg gtgaaccggg tgcataccct tcaccaggaa cagacggaag ttcatcaggt
    56521 taccgtggca ccagcggcgg tcacgtttta gctcatcaag caagttaggc ggtaattctt
    56581 cataagaacc cgggagatcg taagcaatcc agactcccca acctgcacgg cgcatcaacg
    56641 ccgcttccac gaagtcatgt gacaggattg aaccggcaaa ggaaccttcg cccggcagcg
    56701 gggccagtgc acagtgctcg ataaacggtt tcacgcggat aatcgcgtta tgcccccagt
    56761 agtgcgactc gccaagttgc cagaagtgca aaccggctgt aaacagtggc ccatacacgc
    56821 gggtcgcgaa ctgctgacag cgtgcataca gcgtatccat accggacgct ttcggcgacg
    56881 actgaatgat cccggcgttc gggttggctt ccatcaggcg caccaggccg cacaaacaat
    56941 caccggtcat taccgagtca gcatccagca ccaccatata gctgtactgg ctgccccagc
    57001 gacggcagaa gtcatcgata ttaccgcttt tacgcttcac gcgacggcgg cggcggcgat
    57061 agaaaatctg accttcgcca ccgacttcag cgataagctc catccaggct ttttgctctg
    57121 cgacgcagat atccgggttg taactgtcac tcagaatgta gacatcaaag tgtttggcat
    57181 tcccggtggc ttttactgat tcccacgttg cacgcaagcc agcaaaaaca cggttcacgt
    57241 cttcgttaca gataggcatg atcaacgccg tgcgatgctc cgggtttaat ggttcatcgc
    57301 caactgttga cgcagatata ctgtatttat cgcgaccaat aagtagttgc aggaagccca
    57361 ttaacgccgt ccagaatccg gcggacaccc aacagaacag taccgcaaag aggatcagga
    57421 taccggtttg cagcatataa ggcagaagct gcataaagga aacccacaaa tcctgaccaa
    57481 ccatatccat aggattaatc agcgcccaac cctgataagg aagaatggtc ttcatatacc
    57541 aggtcgcgac gacagtttgc gcgagcgtca ggatcaacag aatgtaacgg cggatggtac
    57601 cgacggtacg ccacttttgc tcgctctcct gctcttcttt ggtcaaacga gccagatagc
    57661 gcggtgttac atcgcgtcca cgcaggcgat cccagaaacg gcctaccggg ttggtacgcc
    57721 acgggtcggg aaacatcgag gagcgttttg cttctggcat cgccttaagc tgatcgcgcc
    57781 cttcgtcatc tttaattaac tgtccatcag caagtgaatc tggccaggct tgttccagac
    57841 gcgcctttac cgagccttgc ggggagtcat cctcccgcgc ccaggtgcgg tgttcggcat
    57901 ccagcgcctg atgaacggcg cggatatcag tcttcggcaa tgccgctttc tcgcttgcgg
    57961 cgatgggcat tgcgtcaatg tactcagttg tcttattcat tggcaggtaa ctggtagctc
    58021 caggtttcac tcaacgtctg atcggcattc accagcgcag cgcgcatttc agtggttttc
    58081 ttggcatctt tcactttcac acgcatcacc agacgccagc ctttggtgac cgggttatag
    58141 cgcaccgtgc tttcaactat ctcaccatta tcaccaatgc tggtttgcgc tgtgaccggg
    58201 gtatcctctg gcagtttttt catctctgcg ccggtaaaat cgaccacaaa ggcgatagta
    58261 ccgtcaggct ggcgaatcag gttcgactgc ttcacatccc ccgttgaacg acgcgtttgt
    58321 tgcacccatg cgttatctgg cgcatgcagt ttgtcttcat cacggctgaa ggtgatggtg
    58381 tatttaaagt tcatctcttt acccggctcc ggcagctgat ccggcgtcca gtaagcgacg
    58441 atgttatcgt tagtttcatc gttggttgga atttccacca gctcaacgct gcctttaccc
    58501 cactcccctt tcggagtcac ccatgcgctt ggacggagat cgtaacgatc atcgagatct
    58561 tcaaagcggg agaaatcacg accgcgctgc aacagaccaa agccttgcgg gttttccatg
    58621 gagaagctgc tgaccgctaa atgtttcggg ttattcaacg gacgccagat ccattcgcca
    58681 ttaccggcat ggatagagag accgttagag tcgtgcaact ccggacgata gttatttgct
    58741 ggcgacggtt ggttcggccc aaacaggaac atactggtta acggtgcaac ccccagtttg
    58801 ccgactttat cgcgcagata gattttcgac tgcacatcca caaccgtgtc acgccctggc
    58861 ataactacga atttgtaagc acctgtcgcg cgcggcgagt caagcaatgc ataaatggtt
    58921 aaacgtttat cagttggttt tggacgctcg atccagaact ctttgaagcg tggaaattct
    58981 tcacccgatg gcaaggcggt atcaattgcc agaccgcggg cagaaaggcc ataaacctga
    59041 cctgcaccaa tcacgcggaa atagctggcc ccgagcatgc tgacgatttc atcgttttta
    59101 tctttgctgt tgatcgggta aagcacttta aaaccggcaa aaccaaggtc ttttaccgtg
    59161 tctttgtcat gctgaacatc gccgaaagtg aaataatccg ggctgtattt gattcgtttg
    59221 actgcggtgg cagtcacttc atttattttg accggggtat cgaagtacat accctgatgg
    59281 tagaactcga gtttgaatgg ggtcttcaga ttgttccagt acgctttgtc atgattaaac
    59341 tggatctgct gatagtccgc gtatttcata tcgcggaaaa cggagggcaa gttgcttttg
    59401 ggcgcctcat agcctttccc ggctaaggat tgagcttgct ttgcgacatc atcaatactg
    59461 aaagcccagc ttgaagatgt atacagggtt aacattactg cagcactcaa ccaacgcatt
    59521 ttcatcattt gtagtttatg tttcataatt agtaagcact tccccctttg tgtgcttata
    59581 tcgatccgat ctattttaat ggataaccag ccattccgac aacagaatcc cggtatcgtt
    59641 cagcacgctg gctcatggat taagcacctg attgcaaccg ttttcacttt ccgtgcttct
    59701 cctaaagaca gaagcatcga cttcgtgtag ggtttgccag caaatttatt tatcaaccgt
    59761 cttacagacg cttccattct ggttagtggt agcgatgatg ctatgcataa aaacttgaag
    59821 cctgcgcctg gcgcggtcag gtcggtgtcg ctatggcaac aagcctaaag gcataacccg
    59881 acagggataa gacgaaaaat ccgagattag ttaaccatat atgaatccag tacccgcgca
    59941 acgtgaatat ttcctcgact ccatccgcgc ctggctgatg ttgttaggga tcccttttca
    60001 tatttcttta atctattcga gccatacatg gcatgtgaat agcgccgaac cgtcattgtg
    60061 gctgaccctt tttaatgact tcatccactc gttccgcatg caggtatttt tcgttatatc
    60121 cgggtacttt tcctacatgc tttttttacg ctatcccttg aaaaaatggt ggaaagtacg
    60181 tgtcgaacgt gtagggatcc cgatgttaac agccatcccc ctactgacat tgccgcaatt
    60241 tattatgctg caatacgtca aagggaaagc ggaaagttgg cctgggctgt cattgtatga
    60301 caaatataat acgttggcct gggaattaat atcacatctg tggtttttac tggtgttagt
    60361 ggtcatgacg acgctgtgcg tatggatatt taaacgcatc agaaataatt tagaaaattc
    60421 tgataaaacg aataaaaaat tctcgatggt aaaactatcg gtgatttttt tgtgcctcgg
    60481 catcggttat gcggtaataa gaagaacgat ttttattgtg tatccaccca ttctgagtaa
    60541 tggcacgttc aattttattg tcatgcaaac gctattttat ttaccgttct ttatcctcgg
    60601 cgcactggct ttcattttcc ctcatcttaa agccttgttt accacgccgt ctcgtggctg
    60661 taccctcgct gcagcattgg cgtttgtcgc ttatttactc aaccagcgct atggcagtgg
    60721 cgatgcctgg atgtacgaaa ccgagtcggt gatcaccatg gtcctcggtc tgtggatggt
    60781 gaatgtggtc ttctcctttg gccaccgttt gcttaacttc cagtcagcgc gggtgactta
    60841 ttttgttaac gcatcgctgt ttatctatct ggttcaccac ccgttaacgc tgtttttcgg
    60901 cgcatacatt acaccgcaca tcacctccaa ctggcttggt tttctctgtg gcctgatatt
    60961 cgtagtaggg attgcgataa ttctgtatga aattcatttg cgcatcccgt tactgaagtt
    61021 tttgttctct ggtaaaccgg ttgttaagcg tgagaacgat aaagcaccag cccgttaagc
    61081 cacatttaca ataaccattc cacgggcaat atcgacgcca gtctgaccat aacccgcttc
    61141 cagaaactgg tagcgggttc ttttttgaga acaatctctt tctttgcatg acgatcaacc
    61201 cagttgatcc gtccccacct gtccagacgg agctgccagg ccgcatcata ctggctctga
    61261 ataaagcgtt tatcaattaa ctgagccagc gtttcgctct ctatcacgaa gcccatttca
    61321 gtattgagca atgttgaacg cggatcgaaa ttgaaagaac cgataaacac cgttttacca
    61381 tcgatgctaa aggttttagc atgcaggctg gcaccggaat taccggttat gccgcgatcg
    61441 tgtaacgtac tactttgttc acgcgtcggc ttgagttcat ataattccac gccatagcgg
    61501 agcaattttt tgcgccagcg cgcgtatccg gcatggacga cagcaacatc gttagcggca
    61561 agagaattgg ttaggatcgc aatctttacc ccttttctca ccatccgtaa gagttgcgcc
    61621 acacctgcgc gtgtcggtac aaaataggaa gagataatat cgatgcgttc actgggtgag
    61681 cccatgatat cgaacaggcg ctgcggtaac agtgaatgcc gttttgcctt gccctcccct
    61741 ttcgccggat catcacttaa taaacgtgtt ttcgcccaga taagcggcaa cgttccatca
    61801 accagatgat ttataaacgg actggattcc attttgcgta agtaacgatg cgtcatggca
    61861 tcgttatgcc aggaggcggg taactcgatg cgatccgcca tttcaccttc cgggacatcc
    61921 agcacctgct gtaagggtga aaccgatttg caataccagt agcgggcgaa atcatcggca
    61981 acgtcctcta ctacgggtcc tattgccatg acatctaaat ccgaaaaaag tggctcttcc
    62041 cctgccccaa aataggcatc accaatattt cgccccccta ccaacgtcac cacgccatcg
    62101 acagtgaaac ttttattgtg catacggcga ttgagacggg aaaagtcggt gatataacca
    62161 agcggacgca gcaagcgaaa cgagaaagga ttaaaaagcc ggacttcaat gcggggatgg
    62221 ctgtcaagca agcgtaaaat gtcgtcaagt ccgggcgtat tgttgtcgtc cagcaacaaa
    62281 cggacgcgaa cgccacgctt tgctgcggct aacagggcgg aaaacagtaa ccgacccgac
    62341 atatcgtcct gccagatgta atactgaaca tccagcgtat gctctgacat ttcggccaga
    62401 cgataccggg cggcaaacgc atccagactt ttctccagag ggaaaagacc acactgaccg
    62461 ggatgttgcg aacacagtgg cagcaccgcg ctcgccagcc ggggcaaatc attcatctcc
    62521 ttgttgggta aggagtcttt cgtagaggtg ggcgttttct tcatcataac agacaaagta
    62581 tacctgttcg ggtaaagcgt gacgggtaat aaattctgaa acggttttta ctgcgatttc
    62641 agccgctgcc gcacgagggt aaccataaac cccagtactg attgcaggaa aagccactga
    62701 cgtatagctg tttgccgcca ccagtcgtag gctattgaga taggcatcct gcaaaagctg
    62761 gtcttcgttt tgttcaccac cacgccagac tggcccgacg gtgtgcacta cggctttagc
    62821 aggaagatcg cctgcaagcg taataacggc atgccccgta gggcaatcgc cctgctgttg
    62881 cctgactttt aaacaagcat ccagcagggc cggacccgct gcgcgatgaa tggccccatc
    62941 gacgccgcca cctcccatta atgaaggatt agccgcattc acaatcacat caacggccag
    63001 tttggtaata tcaccctgca caacatgaat acgcgttttc ataacgcctc cttacacctt
    63061 cttgtttctt aagtgtacag caggcttttt ggttaaatcg catttttctg ctcagcaacg
    63121 tgagttgttc agaaacttag ttatattcga tgttcattac agccagcttt ttctgtttat
    63181 caagatactg cacttttact gaggctattt gcgcgttttt gacattgccg ctcattaacg
    63241 ctttgctgga gtaggtttta cccggaacag aaccattctg tgggcagttc atttctacgg
    63301 gtgatgactg ggtgctgacg tcacatgccg gttccacaat ttggccataa aaatggatca
    63361 cgcccccttg cgcggtgctt cctgcgatgg caataaaagg aaaaaacaaa ctgccgagca
    63421 taagagagtt taaaaaaggt ctgaacattt attctatttc ctcaatgatt agtcatcctt
    63481 gagggttgtg ttatccatac tttcaggttg gctgattcgc agcagaccat tctctccaga
    63541 ttcatcttat gctcgatatt tcaacaaatt aagacttttc tgaagagggc ggccattgtt
    63601 gtgataaatg aagtgactgt ccatcagaaa cagtaacaac tattttcacc cgatcgtccg
    63661 gggaaatatt taaactcaac ttcgtcaaag caatgggttg attagcaggc aatgaaaggg
    63721 tcttttcttg cttcgtctga ctttgccctg aactgccttc gcgcagggac aatatttgta
    63781 ctctgcacag acaagattga gtaagagtga cttcaggaat aatggtgtac atatcccctt
    63841 gctgggtcgt attaaaggtt atctgactgg aaagtgccgc aaggagtaat aacgcattca
    63901 tattcttctc ccgaaaaaaa acagggcttg cgccctgttt ctttaataca gatgatgtat
    63961 tagtactgat gagcggtcgc gttgttacca aagccaacct gagttacgtt gacggtggaa
    64021 ttagatgcag tctggtcaac cgctgcaccg ttgccgccac cgaattgttt aactgtcata
    64081 tgagagtctt taccgttcca ctgatcaaga gtggcgctgt taccaaagcc acgttgggtc
    64141 agatcgattg agctgtcatc tgagccctga ccaacatctg caccgttacc accaccatgc
    64201 tgggtaatag taagatcaga gttacgagca tcagcttgca gagcaagtgc agagttacca
    64261 ccaccgtact gataaatatt cagctctgaa ttcgggccgc tgttattacc gccaccaccg
    64321 tggttaccgc caccgccgcc gtactgagga acaacacctg ccagagcgct accggagaat
    64381 acgattgctg caattgctgc tacttttaaa agtttcatgt aaaaccccca tcggattgat
    64441 ttaaaagtcg aatggaaatt aacgttgtgt cacgcgaata gccatttgcg actgtctctg
    64501 cactacaatt gccgtttttt gagtaccata ctgtgtaata tttgctttat taccagaacc
    64561 tttctggata atcatcgcag tattaccata agcaccttgc gaaatactgg catcattggc
    64621 actgcccgcc tgatcaatat atgcaaggtt ataatctcct gtctggtcaa tctttgcccg
    64681 gttgctacta ccttcttgcg caacaaccgc caaaagtttt gagcctccct gccgtaactg
    64741 agcactatta ttagtcccag cttgaccaat tatggctgcc tgattaaatg aagacttact
    64801 caattcattt accgcgaagt tatattctga attagctaaa tcataacctg ctgcggctgc
    64861 aatcccaggc gcacccagta ttgttaacat cataaataac aatttgtttt tcatgttgtc
    64921 accctggacc tggtcgtaca tttaagaaat taaatcattt caacttggtt gttaacgcaa
    64981 cctatatttt gttaacgctg cgttacgatg gaaagtatgt ctgcggaaat atttttaata
    65041 actcacccgc gcgttgtatt tttctttttt tgttcatacc aaagtattaa aacccacgta
    65101 aatacgctgt tatctacgca aaaaatattt tgttttcttt taaatctccg ttttccgcta
    65161 gcaaaaaaca ccagacagtc attcttcttg cccgtcgctg attgctgcga tatgtcttgc
    65221 gcacaagccg tgacaaacct cgtctttttg cagtaaaaaa ttgtccacgg tggtgtggag
    65281 aaaaaacaag aacgttttac atgacgaaag gactacaccg aaatattttt tatatgcatt
    65341 attagtaagt tagcaccatt tgtatgattt tttaaaattg tgcaataaa
//
LOCUS       DAZFDC010000012        60379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFDC010000012 DAZFDC010000000
VERSION     DAZFDC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.012"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(165..1640)
                     /gene="ampG"
                     /locus_tag="ACHJ48_000904"
     CDS             complement(165..1640)
                     /gene="ampG"
                     /locus_tag="ACHJ48_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO4706514.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1684..2262)
                     /gene="yajG"
                     /locus_tag="ACHJ48_000905"
     CDS             complement(1684..2262)
                     /gene="yajG"
                     /locus_tag="ACHJ48_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4706515.1"
                     /translation="MFKKILFPLVALFMLAGCARPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2567..2884
                     /gene="bolA"
                     /locus_tag="ACHJ48_000906"
     CDS             2567..2884
                     /gene="bolA"
                     /locus_tag="ACHJ48_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO4706516.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3228..4526
                     /gene="tig"
                     /locus_tag="ACHJ48_000907"
     CDS             3228..4526
                     /gene="tig"
                     /locus_tag="ACHJ48_000907"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4706517.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4771..5394
                     /gene="clpP"
                     /locus_tag="ACHJ48_000908"
     CDS             4771..5394
                     /gene="clpP"
                     /locus_tag="ACHJ48_000908"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO4706518.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5520..6794
                     /gene="clpX"
                     /locus_tag="ACHJ48_000909"
     CDS             5520..6794
                     /gene="clpX"
                     /locus_tag="ACHJ48_000909"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO4706519.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6982..9336
                     /gene="lon"
                     /locus_tag="ACHJ48_000910"
     CDS             6982..9336
                     /gene="lon"
                     /locus_tag="ACHJ48_000910"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO4706520.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILNTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9527..9817
                     /gene="hupB"
                     /locus_tag="ACHJ48_000911"
     CDS             9527..9817
                     /gene="hupB"
                     /locus_tag="ACHJ48_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO4706521.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10009..11880
                     /gene="ppiD"
                     /locus_tag="ACHJ48_000912"
     CDS             10009..11880
                     /gene="ppiD"
                     /locus_tag="ACHJ48_000912"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO4706522.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADLQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKENLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12031..12402
                     /gene="ybaV"
                     /locus_tag="ACHJ48_000913"
     CDS             12031..12402
                     /gene="ybaV"
                     /locus_tag="ACHJ48_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO4706523.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QNKAAVPAKASDEEGSRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12508..12906
                     /gene="fadM"
                     /locus_tag="ACHJ48_000914"
     CDS             12508..12906
                     /gene="fadM"
                     /locus_tag="ACHJ48_000914"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO4706524.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12958..13653)
                     /gene="queC"
                     /locus_tag="ACHJ48_000915"
     CDS             complement(12958..13653)
                     /gene="queC"
                     /locus_tag="ACHJ48_000915"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO4706525.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKVLNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13718..15418)
                     /gene="ybaE"
                     /locus_tag="ACHJ48_000916"
     CDS             complement(13718..15418)
                     /gene="ybaE"
                     /locus_tag="ACHJ48_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO4706526.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15518..16336
                     /gene="cof"
                     /locus_tag="ACHJ48_000917"
     CDS             15518..16336
                     /gene="cof"
                     /locus_tag="ACHJ48_000917"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO4706527.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16488..16946
                     /gene="decR"
                     /locus_tag="ACHJ48_000918"
     CDS             16488..16946
                     /gene="decR"
                     /locus_tag="ACHJ48_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO4706528.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16976..18748
                     /gene="mdlA"
                     /locus_tag="ACHJ48_000919"
     CDS             16976..18748
                     /gene="mdlA"
                     /locus_tag="ACHJ48_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4706529.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            18741..20522
                     /gene="mdlB"
                     /locus_tag="ACHJ48_000920"
     CDS             18741..20522
                     /gene="mdlB"
                     /locus_tag="ACHJ48_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO4706530.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20703..21041
                     /gene="glnK"
                     /locus_tag="ACHJ48_000921"
     CDS             20703..21041
                     /gene="glnK"
                     /locus_tag="ACHJ48_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO4706531.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21071..22357
                     /gene="amtB"
                     /locus_tag="ACHJ48_000922"
     CDS             21071..22357
                     /gene="amtB"
                     /locus_tag="ACHJ48_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO4706532.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22406..23266)
                     /gene="tesB"
                     /locus_tag="ACHJ48_000923"
     CDS             complement(22406..23266)
                     /gene="tesB"
                     /locus_tag="ACHJ48_000923"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO4706533.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23484..24056
                     /gene="ybaY"
                     /locus_tag="ACHJ48_000924"
     CDS             23484..24056
                     /gene="ybaY"
                     /locus_tag="ACHJ48_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO4706534.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24089..24400)
                     /locus_tag="ACHJ48_000925"
     CDS             complement(24089..24400)
                     /locus_tag="ACHJ48_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO4706535.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24563..24659
                     /gene="ffs"
                     /locus_tag="ACHJ48_000926"
     ncRNA           24563..24659
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHJ48_000926"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24779..25132
                     /gene="ybaA"
                     /locus_tag="ACHJ48_000927"
     CDS             24779..25132
                     /gene="ybaA"
                     /locus_tag="ACHJ48_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO4706536.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25174..26724)
                     /gene="pdeB"
                     /locus_tag="ACHJ48_000928"
     CDS             complement(25174..26724)
                     /gene="pdeB"
                     /locus_tag="ACHJ48_000928"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO4706537.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASSKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26888..27358)
                     /gene="ylaC"
                     /locus_tag="ACHJ48_000929"
     CDS             complement(26888..27358)
                     /gene="ylaC"
                     /locus_tag="ACHJ48_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO4706538.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27474..28025)
                     /gene="maa"
                     /locus_tag="ACHJ48_000930"
     CDS             complement(27474..28025)
                     /gene="maa"
                     /locus_tag="ACHJ48_000930"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO4706539.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28197..28415)
                     /gene="hha"
                     /locus_tag="ACHJ48_000931"
     CDS             complement(28197..28415)
                     /gene="hha"
                     /locus_tag="ACHJ48_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO4706540.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28441..28815)
                     /gene="tomB"
                     /locus_tag="ACHJ48_000932"
     CDS             complement(28441..28815)
                     /gene="tomB"
                     /locus_tag="ACHJ48_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO4706541.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29361..32510)
                     /gene="acrB"
                     /locus_tag="ACHJ48_000933"
     CDS             complement(29361..32510)
                     /gene="acrB"
                     /locus_tag="ACHJ48_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO4706542.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSIEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(32533..33726)
                     /gene="acrA"
                     /locus_tag="ACHJ48_000934"
     CDS             complement(32533..33726)
                     /gene="acrA"
                     /locus_tag="ACHJ48_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO4706543.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33868..34515
                     /gene="acrR"
                     /locus_tag="ACHJ48_000935"
     CDS             33868..34515
                     /gene="acrR"
                     /locus_tag="ACHJ48_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO4706544.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPSDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34643..38005
                     /gene="mscK"
                     /locus_tag="ACHJ48_000936"
     CDS             34643..38005
                     /gene="mscK"
                     /locus_tag="ACHJ48_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO4706545.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(38044..38208)
                     /gene="rsmS"
                     /locus_tag="ACHJ48_000937"
     CDS             complement(38044..38208)
                     /gene="rsmS"
                     /locus_tag="ACHJ48_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO4706546.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(38222..38749)
                     /gene="priC"
                     /locus_tag="ACHJ48_000938"
     CDS             complement(38222..38749)
                     /gene="priC"
                     /locus_tag="ACHJ48_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO4706547.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFNRHLFQTRAT
                     TLQACLDKAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PQIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38819..39196
                     /gene="ybaN"
                     /locus_tag="ACHJ48_000939"
     CDS             38819..39196
                     /gene="ybaN"
                     /locus_tag="ACHJ48_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO4706548.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39349..39900
                     /gene="apt"
                     /locus_tag="ACHJ48_000940"
     CDS             39349..39900
                     /gene="apt"
                     /locus_tag="ACHJ48_000940"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO4706549.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGINKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            40029..41960
                     /gene="dnaX"
                     /locus_tag="ACHJ48_000941"
     CDS             40029..41960
                     /gene="dnaX"
                     /locus_tag="ACHJ48_000941"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO4706550.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEDSIRPI"
     misc_feature    41293..41357
                     /gene="dnaX"
                     /locus_tag="ACHJ48_000941"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            42013..42342
                     /gene="ybaB"
                     /locus_tag="ACHJ48_000942"
     CDS             42013..42342
                     /gene="ybaB"
                     /locus_tag="ACHJ48_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO4706551.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            42342..42947
                     /gene="recR"
                     /locus_tag="ACHJ48_000943"
     CDS             42342..42947
                     /gene="recR"
                     /locus_tag="ACHJ48_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO4706552.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            43057..44931
                     /gene="htpG"
                     /locus_tag="ACHJ48_000944"
     CDS             43057..44931
                     /gene="htpG"
                     /locus_tag="ACHJ48_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO4706553.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            45112..45756
                     /gene="adk"
                     /locus_tag="ACHJ48_000945"
     CDS             45112..45756
                     /gene="adk"
                     /locus_tag="ACHJ48_000945"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO4706554.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            45888..46850
                     /gene="hemH"
                     /locus_tag="ACHJ48_000946"
     CDS             45888..46850
                     /gene="hemH"
                     /locus_tag="ACHJ48_000946"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO4706555.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(46847..47806)
                     /gene="aes"
                     /locus_tag="ACHJ48_000947"
     CDS             complement(46847..47806)
                     /gene="aes"
                     /locus_tag="ACHJ48_000947"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO4706556.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATKAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQVIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            47958..49262
                     /gene="gsk"
                     /locus_tag="ACHJ48_000948"
     CDS             47958..49262
                     /gene="gsk"
                     /locus_tag="ACHJ48_000948"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO4706557.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(49395..51071)
                     /gene="ybaL"
                     /locus_tag="ACHJ48_000949"
     CDS             complement(49395..51071)
                     /gene="ybaL"
                     /locus_tag="ACHJ48_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO4706558.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(51308..52528)
                     /gene="fsr"
                     /locus_tag="ACHJ48_000950"
     CDS             complement(51308..52528)
                     /gene="fsr"
                     /locus_tag="ACHJ48_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO4706559.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            52746..54398
                     /gene="ushA"
                     /locus_tag="ACHJ48_000951"
     CDS             52746..54398
                     /gene="ushA"
                     /locus_tag="ACHJ48_000951"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO4706560.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(54435..54914)
                     /gene="ybaK"
                     /locus_tag="ACHJ48_000952"
     CDS             complement(54435..54914)
                     /gene="ybaK"
                     /locus_tag="ACHJ48_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO4706561.1"
                     /translation="MTPAVKSLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(55118..55912)
                     /gene="ybaP"
                     /locus_tag="ACHJ48_000953"
     CDS             complement(55118..55912)
                     /gene="ybaP"
                     /locus_tag="ACHJ48_000953"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO4706562.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            56050..56391
                     /gene="ybaQ"
                     /locus_tag="ACHJ48_000954"
     CDS             56050..56391
                     /gene="ybaQ"
                     /locus_tag="ACHJ48_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4706563.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(56606..59110)
                     /gene="copA"
                     /locus_tag="ACHJ48_000955"
     CDS             complement(56606..59110)
                     /gene="copA"
                     /locus_tag="ACHJ48_000955"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO4706564.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            59372..60304
                     /gene="glsA"
                     /locus_tag="ACHJ48_000956"
     CDS             59372..60304
                     /gene="glsA"
                     /locus_tag="ACHJ48_000956"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO4706565.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            60307..>60379
                     /locus_tag="ACHJ48_000957"
     CDS             60307..>60379
                     /locus_tag="ACHJ48_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706566.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIG"
BASE COUNT        15003 a        15246 c        16015 g        14115 t
ORIGIN      
        1 actcattagc tgaatcgtgt aagaaaaata gaatttataa atggcgccaa taatttccaa
       61 acgctaagcc gcacaaaaga acaaatatta ataagcgatg ctgatgttta tttagccgta
      121 ataattacgg cgagtgatta ctacagctaa ataatattta cagattacgt cagatgcgtt
      181 tttcgtagcg ccagataatc cagcaaacca ccaagcacga cgccagaaag ggcgactaaa
      241 accccgactt ccagcagagc aggcaggaaa gagaagtgcg tcaaatccag cgcgtccatc
      301 gtcaatagca gtaaccacac ggccaacagg ctgacgcccg ccgccagtgt ccacatggca
      361 aaggcataac ctgccggata tgcggtacgg gagataaagt tgtcatttac tcgtgtatat
      421 tcaagcgtct ggcggcaaac cagcagcaaa ataagccctg gtacggcagc ggcgacggag
      481 aatagataga atgtcgacca gccgtgtgct tcaacaaacc aacccgccac ggggccgaca
      541 taaactcgcc ctacagcaga aagcgctgag agcagggcaa attgagtagc ggaaaatgac
      601 ttattacata gcgtcattaa cagcgcgaca aaggctgatg tgcccatccc gccacagagg
      661 ttttcgaaaa agacggctgc gcccatgctg tagagatgct tatcagtaat cgacagcagc
      721 cagtaaccag cgttagacgc accttgtaaa atgccgaaaa tcagcagtgc ccggaacagt
      781 gacaggcgct gcatcaaaat cccaccgtac aatgcgccaa caatggtcgc tagtaagcca
      841 agcgttttgt ttaccacgcc tacttcaccc gcatcaaacc cgacgccgcg aatcaaaaac
      901 gtggttgtca ggctcatggc gaatgcgtcg cccagcttat acagcacgat aagaagcaaa
      961 ataagccagg cattattgcg accaaagaaa tctcgcagag gtgcaacaac cgcttgttcc
     1021 agcgttttgg gcacaggaat ggtgtcggtt ggttctggtg caagcaacgt cgcgataata
     1081 caggggatca acagcgccgc catcaaccag tacatgccct gccagcccag ccatttatct
     1141 gccagccaca gggctaggcc gccggaaacc agcatcccta aacggtaacc cagaacgctg
     1201 attgccgcgc ccgcaccacg ttcttctgcc gggagcacat cggttttcca cgcatcgaag
     1261 acaatatcct gggaggcaga gcaaaaagcg atcaccaccg ccagcgccgc catccagcgg
     1321 agttgggtgc ctggttcgag aaaacccatc gccgcaatgg cgactaataa caggatttgc
     1381 gtggcgagca gccaaccgcg ccgccgccca aaaaatgggg gcgtgtagcg gtccatcagc
     1441 ggtgaccaga ggaatttaaa aacgtaagcc tggcctacca gagagaagaa accaatggtt
     1501 ttgagatcga tattctcgac cgtcatccag gcctgtaagg tgccggatgt cagggcgagc
     1561 ggtagcccgg aagcaaaacc caggatcagc aatatggctg aacgcggctg ttgaaaaata
     1621 cgtaaatatt gactggacat gggcgtctac aggcccggct tgcaccgggc cagagggcag
     1681 aaattaacgc gcgttctgct tgatgaattc gtgaatgctg gtgtcctgag acatatcagc
     1741 gatggtatcg gtcagcacac tgttaaccgc atcggcgata tttttgttgg aggcctggaa
     1801 cgcaccttca acgttgtagc tggcacgata gtttttggtc attttgttgc cattctgcgc
     1861 ggtagcgatg atggcgatat ccgctttggt agcgatgttg tagcgcacgt tgccctggga
     1921 cacgtcagca tacagctggc taacgatgat ttgcagatta accgggccat tcggaccaac
     1981 catgtaacca cgcgcggtca tctgtttttc cagcacttct tgcagcagga aacgcagatc
     2041 gcgggaggcg gtcagggtaa cgatttgatt atcgcgggtg acttttgcca gcgcctgatc
     2101 ggtacgctga tcggcaccat taatgcttac ggtgacgccc atcaggcttg gatcctgctg
     2161 tggcagtgta atcgtcgggg aaacttcaat agttgttggc ggtcttgcgc atcctgcaag
     2221 cataaacaga gcaactaacg ggaagaggat ttttttgaac atgttcgggc tctcagagac
     2281 tcttaagcgt gtttggtaaa aattcccgcc atcataacat tgccaacggc gaggggaagt
     2341 aggtaaggca tgtaaattca tcatgttgac gaaataatcg cccctggtaa aagaaacgct
     2401 gatgcgaggc ctgtgtttca atctttaaat cagtaaactt catacgcttg acggaaaaac
     2461 caggacgaaa cctaaatatt tgttgttaag ctgcaatgga aacggtaaaa gcggctagta
     2521 tttaaaggga tggatgacat ctcagcgttg tcggaggaga tatttcatga tgatacgtga
     2581 gcggatagaa gaaaaattaa gggcggcgtt ccaacccgta ttcctcgaag tagtggatga
     2641 aagctatcgt cacaatgtcc cagccggctc tgaaagccat tttaaagttg tgctggtcag
     2701 cgatcgtttt acgggtgaac gttttctgaa tcgtcatcga atgatttaca gtactttagc
     2761 ggaggaactc tctactaccg ttcatgcgct ggctctgcat acttacacta ttaaggagtg
     2821 ggaagggttg caggacaccg tctttgcctc tcctccctgt cgtggagcag gaagcatcgc
     2881 gtaaaaacgc atttgcaact gtcggcgctt ttccagtatg ttgctaaaga ttttatgaaa
     2941 aacggcctgc gggccgtttt gttttgtctg gattttgcgc tttttgccca gcattcagac
     3001 gaaaattgcc cgggaattgt gaaaaaatac gcgacagcgc gcaataaccg ttctcgactc
     3061 ataaaagtga tgccgctata atgccgcgtc ctatttgaat gctttcggga tgattctggt
     3121 aacagggaat gtgattgatt ataagaacat cccggttccg cgaagccaac aacctgtgct
     3181 tgcggggtaa gagttgaccg agcactgtga ttttttgagg taacaagatg caagtttcag
     3241 ttgaaaccac tcaaggcctt ggccgccgtg taacgattac tatcgctgct gacagcatcg
     3301 agaccgctgt taaaagcgag ctggtcaacg ttgcgaaaaa agtacgtatt gacggcttcc
     3361 gcaaaggcaa agtgccaatg aatatcgttg ctcagcgtta tggcgcgtct gtacgccagg
     3421 acgttctggg tgacctgatg agccgtaact tcattgacgc catcattaaa gaaaaaatca
     3481 atccggctgg agcaccgact tatgttccgg gcgaatacaa gctgggtgaa gacttcacct
     3541 actctgtaga gtttgaagtt tatccggaag ttgaactgca gggtctggaa gcgatcgaag
     3601 ttgaaaaacc gatcgttgaa gtgaccgacg ctgacgttga cggcatgctg gatactctgc
     3661 gtaaacagca ggcgacctgg aaagaaaaag acggcgctgt tgaagcagaa gaccgcgtaa
     3721 ccatcgactt caccggttct gtagacggcg aagagttcga aggcggtaaa gcgtctgatt
     3781 tcgtactggc gatgggccag ggtcgtatga tcccgggctt tgaagacggt atcaaaggcc
     3841 acaaagctgg cgaagagttc accatcgacg tgaccttccc ggaagaatac cacgcagaaa
     3901 acctgaaagg taaagcagcg aaattcgcta tcaacctgaa gaaagttgaa gagcgtgaac
     3961 tgccggaact gactgcagaa ttcatcaaac gtttcggcgt tgaagatggt tccgtagaag
     4021 gtctgcgcgc tgaagtgcgt aaaaacatgg agcgcgagct gaagagcgcc atccgtaacc
     4081 gcgttaagtc tcaggcgatc gaaggtctgg taaaagctaa cgacatcgat gtaccggctg
     4141 cgcttattga cagcgaaatc gacgttctgc gtcgccaggc tgcacagcgt ttcggtggca
     4201 acgaaaaaca agctctggaa ctgccgcgcg aactgttcga agaacaggct aaacgccgcg
     4261 tagttgttgg cctgctgctg ggcgaagtta tccgcaccaa cgagctgaaa gctgacgaag
     4321 agcgcgtgaa aggcctgatc gaagagatgg cttcagcgta cgaagatccg aaagaagtta
     4381 tcgagttcta cagcaaaaac aaagaactga tggacaacat gcgcaatgtt gctctggaag
     4441 aacaggctgt tgaagctgta ctggcgaaag cgaaagtgac tgaaaaagaa accactttca
     4501 acgagctgat gaaccagcag gcgtaattta cgcagcataa cgcgctaaat tcgcacaaag
     4561 gcccgtcacc gccaggtggt gggctttttt tgtcatgaat tttgcatgga accgtgcgaa
     4621 aagcctcttt cggtgttagc gtaacaacaa aagattgtta tgcttgaaat atggtgatgc
     4681 cgtacccata acacagggac tagctgataa tccgtccata aggttacaat cggtacagca
     4741 ggttttttca attttatcca ggagacggaa atgtcataca gcggcgaacg agataacttt
     4801 gcaccccata tggcgctggt gccgatggtc attgaacaga cctcacgtgg tgagcgctct
     4861 tttgatatct attctcgtct acttaaggaa cgcgtcattt ttctgactgg ccaggttgaa
     4921 gaccatatgg ctaacctgat tgtggctcag atgctgttcc tggaagcaga aaacccagaa
     4981 aaagatatct atctgtacat taactcccca ggcggggtga tcactgccgg gatgtctatc
     5041 tatgacacca tgcagtttat caagcctgat gtcagcacca tctgtatggg ccaggcggcc
     5101 tcgatgggcg ctttcttgct gaccgcaggg gcaaaaggta aacgtttttg cctgccgaat
     5161 tcgcgcgtga tgattcacca accgttgggc ggctaccagg gccaggcgac cgatatcgaa
     5221 attcatgccc gtgaaattct gaaagttaaa gggcgtatga atgaacttat ggcgcttcat
     5281 acgggtcaat cattagaaca gattgaacgt gataccgagc gcgatcgctt cctttccgcc
     5341 cctgaagcgg tggaatacgg tctggtcgat tcgattctga cccatcgtaa ttgatgccag
     5401 aggcgcaact gtgccgctat acttatccag ggcggcacaa cgctgtaagc ggcttgcgcc
     5461 tgagaatggc atttgcgtcg tcgtgtgcgg cacaaagaac aaagaagagg ttttgaccca
     5521 tgacagataa acgcaaagat ggctcaggca aattgctgta ttgctctttt tgcggcaaaa
     5581 gccagcatga agtgcgcaag ctgattgccg gtccatccgt gtatatctgc gacgaatgtg
     5641 ttgatttatg taacgacatc attcgcgaag agattaaaga agttgcaccg catcgtgaac
     5701 gcagtgcgct accgacgccg catgaaattc gtaaccacct ggacgattac gttatcggcc
     5761 aggaacaggc gaaaaaagtg ctggcggtcg cggtatacaa ccactacaaa cgtctgcgca
     5821 acggcgatac cagcaatggc gtcgagttgg gcaaaagtaa cattctgctg atcggtccga
     5881 ccggttccgg taaaacgctg ctggctgaaa cgctggcgcg cctgctggac gtcccgttca
     5941 ccatggccga cgcaaccacg ctgaccgaag ccggttatgt gggcgaagac gttgaaaaca
     6001 tcattcagaa gctgttgcaa aagtgcgatt acgacgtaca gaaagcgcag cgcgggattg
     6061 tctacatcga tgaaatcgac aagatttctc gtaagtcaga caacccgtct attacccgtg
     6121 acgtttccgg tgaaggcgta cagcaggcac tgttgaaact gatcgaaggt acggtagctg
     6181 ctgttccacc gcaaggtgga cgtaaacatc cgcagcagga attcttgcag gttgatacct
     6241 ctaagatcct gtttatttgt ggcggtgcgt ttgccggtct ggataaagtg atttcccatc
     6301 gtgtagaaac cggctccggc attggttttg gcgcgacggt aaaagcgaag tccgacaaag
     6361 caagcgaagg cgagctgctg gcgcaggttg aaccggaaga tctgatcaag tttggtctta
     6421 tccctgagtt tattggtcgt ctgccggttg tcgcaacgtt gaatgaactg agcgaagaag
     6481 ctctgattca gatcctcaaa gagccgaaaa acgccctgac caagcagtat caggcgctgt
     6541 ttaatctgga aggcgtggat ctggaattcc gtgacgaggc gctggatgct atcgctaaga
     6601 aagcgatggc gcgtaaaacc ggtgcccgtg gcctgcgttc catcgtagaa gccgcactgc
     6661 tcgataccat gtacgatctg ccgtccatgg aagatgtcga aaaagtggtt atcgacgagt
     6721 cggtaattga tggtcaaagc aaaccgttgc tgatttatgg caagccggaa gcgcaacagg
     6781 catctggtga ataattaacc attcccatac aattagttaa ccaaaaaggg gggattttat
     6841 ctccccttta atttttcctc tattctcggc gttgaatgtg ggggaaacat ccccatatac
     6901 tgacgtacat gttaatagat ggcgtgaagc acagtcgtgt catctgatta cctggcggaa
     6961 attaaactaa gagagagctc tatgaatcct gagcgttctg aacgcattga aatccccgta
     7021 ttgccgctgc gcgatgtggt ggtttatccg cacatggtca tccccttatt tgtcgggcgg
     7081 gaaaaatcta tccgttgtct ggaagcggcg atggaccatg ataaaaaaat tatgctggtc
     7141 gcgcagaaag aagcttcaac ggatgagccg ggtgtaaacg atcttttcac cgtcgggacc
     7201 gtggcctcta tattgcagat gctgaaactg cctgacggca ccgtcaaagt gctggtcgag
     7261 gggttacagc gcgcgcgtat ttctgcgctc tctgacaatg gcgaacactt ttctgcgaag
     7321 gcggagtatc tggagtcgcc gaccattgat gagcgagaac aggaagtgct ggtgcgtact
     7381 gcaatcagcc agttcgaagg ctacatcaag ctgaacaaaa aaatcccacc agaagtgctg
     7441 acgtcgttga atagcatcga cgatccggcg cgtctggcgg ataccattgc tgcacatatg
     7501 ccgctgaaac tggctgacaa acagtccgtt ctggagatgt ccgacgttaa cgaacgtctg
     7561 gaatatctga tggcaatgat ggaatcggaa atcgatctgc tgcaggttga gaaacgcatt
     7621 cgcaaccgcg ttaaaaagca gatggagaaa tcccagcgtg agtactatct gaacgagcaa
     7681 atgaaagcta ttcagaaaga actcggtgag atggacgacg cgccggacga aaacgaagcc
     7741 ctgaagcgca aaatcgacgc ggcgaagatg ccgaaagagg caaaagagaa agcggaagca
     7801 gagttgcaga agctgaaaat gatgtctccg atgtcggcag aagcgaccgt agtgcgtggt
     7861 tatatcgact ggatggtaca ggtaccgtgg aatgcgcgca gcaaggtcaa aaaagacctg
     7921 cgtcaggcgc aggaaatcct taataccgac cattatggtc tggagcgcgt gaaagatcgc
     7981 atccttgagt atcttgcggt tcaaagccgt gtcaacaaaa tcaagggacc gatcctttgc
     8041 ctggtagggc cgccgggggt aggtaaaacc tccctgggtc agtccattgc caaagccacc
     8101 gggcgtaaat atgtccgtat ggcgctgggc ggcgtgcgtg atgaagcgga aatccgtggt
     8161 caccgccgta cttacatcgg ttctatgccg ggtaaattga tccagaaaat ggcgaaagtg
     8221 ggcgttaaaa acccgctgtt cctgctcgat gagatcgaca aaatgtcttc tgacatgcgt
     8281 ggcgatccgg cttccgcact gcttgaagtg ctggatccag agcagaacgt ggccttcagc
     8341 gatcactacc tggaagtgga ttacgatctc agcgacgtga tgtttgtcgc gacgtcgaac
     8401 tccatgaaca ttccggcacc actgctggat cgtatggaag tgattcgcct ctccggttat
     8461 accgaagatg aaaaactgaa catcgccaaa cgtcacctgc tgccgaagca gattgaacgt
     8521 aatgcactga aaaaaggtga gctgaccgtc gacgatagcg ccattatcgg cattattcgt
     8581 tactacaccc gtgaggcggg cgtgcgtggt ctggagcgtg aaatctccaa gctgtgccgt
     8641 aaagcggtta agcagttact gctcgataag tcattaaaac atatcgaaat taacggcgac
     8701 aacctgcatg actaccttgg tgttcagcgt ttcgactatg gtcgcgctga taacgaaaac
     8761 cgtgtcggtc aggtaaccgg tctggcgtgg acggaagtgg gcggtgactt gctgaccatt
     8821 gaaaccgcat gtgttccggg taaaggcaaa ctgacctata ccggttcgct cggcgaagtg
     8881 atgcaggagt ctattcaggc tgcgttaacg gtggttcgcg cgcgtgcgga aaaactgggg
     8941 atcaaccctg atttttacga aaaacgtgac atccacgtcc acgtaccgga aggtgcgacg
     9001 ccgaaagatg gtccgagtgc cggtattgct atgtgcaccg cgctggtttc ttgcctgacc
     9061 ggtaacccgg ttcgtgccga tgtggcaatg accggtgaga tcactctgcg tggtcaggta
     9121 ctgccgatcg gtggtttgaa agaaaaacta ctggcagcgc atcgcggcgg gattaaaaca
     9181 gtgttaattc cgttcgaaaa taaacgcgat ctggaagaga ttcctgacaa cgtaattgcc
     9241 gatctggaca ttcatcctgt gaagcgcatt gaggaagttc tgactctggc gctgcaaaat
     9301 gaaccgtctg gcatgcaggt tgtgactgca aaatagtgac ctcgcgcaaa atgcactaat
     9361 aaaaacaggg ctggcaggct aattcgggct tgccagcctt tttttgtctc gctaagttag
     9421 atggcggatc gggcttgccc ttattaaggg gtgttgtaag gggatggctg gcctgatata
     9481 actgctgcgc gttcgtacct tgaaggattc aagtgcgata taaattataa agaggaagag
     9541 aagagtgaat aaatctcaat tgatcgacaa gattgctgca ggggctgata tctctaaagc
     9601 tgcggctggc cgtgcgttag atgctattat tgcttccgta actgaatctc tgaaagaagg
     9661 ggatgatgta gcactggtag gttttggtac ttttgccgtt aaagagcgtg ctgcccgtac
     9721 tggccgcaac ccgcagaccg gtaaagagat caccatcgct gccgctaaag taccgagctt
     9781 ccgtgcaggt aaagcactga aagacgcggt aaactaagcg ttgtccccag tggggatgtg
     9841 acgaagttca agggcgcatc tactgatgtg ccttttttat ttgtattcgg tgactttctg
     9901 cgtcttgtgg gctgacaatt gcccccgttt cttgtcacaa taggcctttg cgcgcatcga
     9961 tacgttgcgt gaggtacaca gtcatctaca gcggagtgtt gttacaccat gatggacagc
    10021 ttacgcacgg ctgcaaacag tctcgtgctc aagattattt tcggtatcat tatcgtgtcg
    10081 ttcatattga ccggcgtgag tggttacctg attggcggag gcaataacta cgccgcaaaa
    10141 gtgaatgacc aggaaatcag ccgtgggcaa ttcgagaatg ccttcaacag cgagcgtaat
    10201 cgcatgcagc aacagctggg cgatcaatac tccgagctgg cagcgaacga aggctatatg
    10261 aaaaccctgc gtcaacaggt gctgaatcgt ctgatcgacg aggcgctgct ggatcagtac
    10321 gcacgtgagc tgaaactggg tatcagcgat gagcaggtta aacaggcgat tttcgcgacc
    10381 ccagccttcc aggttgatgg caaatttgat aacagccgct ataacggtat cctcaaccag
    10441 atggggatga ccgccgatca gtacgcccag gcgctgcgta accagctcac tacccaacag
    10501 ctgattaacg gcgttgctgg taccgatttt atgctgaaag gtgaaaccga cgagctggcg
    10561 gcactggtcg ctcaacaacg cgtggtgcgt gaagcgacta tcgatgttaa cgcgctggcg
    10621 gcgaagcagc ctgtgaccga acaggaaatt gccagctact acgaacaaaa caaaaacaat
    10681 ttcatgacgc cggaacaatt ccgcgtgagt tacatcaagc tggatgccgc aacgatgcag
    10741 caaccggtta gcgatgcgga tctccagagc tactacgatc agcatcagga tcaattcacc
    10801 cagccgcagc gtacccgtta cagcatcatc cagaccaaaa ctgaagatga agcgaaagcg
    10861 gtacttgatg agctgaataa aggcggtgat tttgctgcat tagccaaaga aaaatctgcc
    10921 gatattatct ctgctcgtaa cggcggcgat atgggttggt tagaagatgc cactatcccg
    10981 gatgaactga aaaatgctgg tctgaaagaa aaaggccaac tgtctggtgt catcaaatct
    11041 tcggtcggtt tcctgattgt acgtctggac gacattcagc cagcgaaagt gaaatcgtta
    11101 gacgaagtac gtgacgatgt cgcggcgaaa gtgaaacacg aaaaagccct cgatgcgtac
    11161 tacgcgctgc agcagaaagt gagcgatgcg gcaagcaacg acaccgagtc tctggccggt
    11221 gcagagcaag ctgccggcgt taaagccact cagacgggtt ggttcagcaa agagaacctg
    11281 ccggaagagt tgaacttcaa gccggttgct gacgctatct ttaacggcgg tctggtaggt
    11341 gaaaacggcg cgccgggcat caactctgac atcatcaccg tagacggcga ccgcgcattc
    11401 gtgctgcgca tcagcgagca caaaccggaa gcggtgaaac cgttggcaga tgttcaggaa
    11461 caagttaagg cactggttca gcacaacaaa gctgaacaac aggcgaaagt ggatgctgag
    11521 aaactgctgg ttgatttgaa agccggcaaa ggtgcggaag ctatgcaggc tgccggtctg
    11581 aaatttggcg agccgaaaac cttaagccgt tccggtcgtg acccgattag ccaggcggcg
    11641 tttgcactgc cactgccagc gaaagacaaa ccgagctacg gtatggcgac cgatatgcaa
    11701 ggtaatgtgg ttctgctggc gctggatgaa gtgaaacaag gttcaatgcc ggaagatcag
    11761 aaaaaagcga tggtgcaggg tatcacccag aacaacgcac aaatcgtctt tgaagctctg
    11821 atgagtaacc tgcgtaaaga ggcgaaaatc aaaattggcg atgcgttgga acagcaataa
    11881 tcctgaagcc gcctcgcaaa aaaatgcttt gcagctgtaa caagaaaagg tcgctttcgc
    11941 ggccttttcc atttctgaac attgccattt gtttactgtt ttcactgccg ttaaggtgat
    12001 tccactgtta acaaacaagg agaaaacagt atgaaacacg gaattaaagc actgctcatt
    12061 accctgtccc tggcctgtgc cggaatgtct catagcgcgc tggcggcagc ttctgtggcg
    12121 aaaccgacgg cggtagaaac caaagcggaa gctcctgcag cacaaaataa agcagcagta
    12181 ccggcgaaag ccagtgacga agaaggctcc cgggtcagca ttaataatgc cagcgcggaa
    12241 gagctagccc gcgcgatgaa tggcgttggc ctgaagaaag cgcaagcgat tgtcagttat
    12301 cgcgaagagt acggtccgtt taaaacggtg gaggatctaa agcaggtgcc ggggatgggc
    12361 aattcgctgg tggaacgtaa tctggcggta ttaaccctgt aattaatttg catagtggca
    12421 attttttgcc agactgaaga ggtcatacca gttatgacct ctgtacttat aacagttttt
    12481 ataaaaacaa cgtaaggtta ttgcgctatg caaacacaaa tcaaagttcg tggatatcat
    12541 ctcgacgttt accagcacgt aaacaacgcc cgctaccttg aatttctcga agaagcccgc
    12601 tgggatgggt tggaaaatag cgacagtttt cactggatga cggcccataa catcgctttc
    12661 gtggtggtga atatcaatat taactatcgt cgtccggcgg tattaagcga cttgttaacc
    12721 attacgagcc agttacagca attaaacggt aaaagcggca tcttaagcca ggtcattaca
    12781 ctggagccgg aagggcaggt ggtagcggat gcgcttatta cgtttgtttg tattgatctt
    12841 aaaacgcaga aagcattagc tctggaaggg gaattgcgcg aaaagctgga gcagatggtt
    12901 aagtaaacgt tttgtggtgc cggatgctca agccgcatcc ggcgacaccc ggaataatta
    12961 cttcaacccg gttttctgct tcatcgctgc catcaccgtc ggtttatcgg ccagataatg
    13021 attcaaaccg ttggcgcgta aattacatgc cgcacaatga ccgcaaccgt cgcctttaat
    13081 gccgttatag caggtcaacg tttcgttacg gactaaatcc agtttgccgt aataatctgc
    13141 cagcgcccag gtttccgctt tatcaatcca catcagcggc gtttcaaaac gaatatcttt
    13201 cgccatgccc aaactgacgg catggtttag tactttcaca aactcatcgc ggcaatccgg
    13261 gtagccggag aaatccgttt cgcagacgcc ggtaattacg gcttctgctt ttacctgata
    13321 cgcatatatt gccgccagcg tcaggaacaa aatattacgc cctgggacaa acgtattcgg
    13381 gatgccatcg gcttcaggtt cataatcagg caccggaatg ctgtcacgcg tcaggctgct
    13441 gaccgccagc tcgttgagca gcgttacatc aagaacctta tgcgcgcgtg cccccagttt
    13501 cagcgccagt tcgcgtgcca cgtcgatttc tgcgcgatgg cgctgaccgt aatcgaacgt
    13561 cacgcaatgg acttcatcat attgttgtaa tgcctgcacc agacaggtgg tggaatcctg
    13621 gcctccactg aacacaacga cagcacgttt catagataat cctgcttgaa caataaaagc
    13681 gttatggtaa cgcctgcgat taacccggac cagctattca ttgcgacggc gcgggaagcc
    13741 aggcttcggt aaattcaaac cagccgcgcg gtgtcagtcg cacaccgttc acgccgggag
    13801 gggcactaat gcgatagtga tagttgaaca gcggcgtcag cgtcgcatct gtcattaact
    13861 ggctaaaaac cgctttcagg gcattaaatc ggttttcctc atcaggcatt acttgcaccg
    13921 cgtccagtgt cgattgtaga tgtgcgtatg ctggagcgtc gaaaacatgt ggccacagcg
    13981 gatcgcagcg cagccattgc tccagagtat attccggtgc ttcgccaatt aatctgtcgc
    14041 ccatcatgag gtctgcgtgt gccagtaggg tcgtgtcgtc ccagtttttt gcgttatgaa
    14101 aaataattgt gagttcacag ccttccgctg ccagtgtcgc ctgtaggcgt tctgccatgg
    14161 tatgaagttc tatcgggagg tgataaacca gcgtcaaggt tttcggtagt ttgacttcat
    14221 ccggtacttg ccaatgcgga atagtccagc ctggcagtaa tgcatgactg gcggtgatca
    14281 ggttttctcc gacttctaac gtttgtaata aaccggattg atgaataatg gagatcactt
    14341 ttcgcgcctg ccagagggag agtcggggac ttttgcgcaa cgttaaatag caaaaaccta
    14401 aactgatgcc actactgacc tggctgaccc gttgcagctc ctccggtttg ccgatggtga
    14461 tttgcacggg atgccgacaa ctggttccca aatctttttc gaaaagcggc ggagttatcc
    14521 agtactcaac cgctttaagc agcggatgac gtaaatggta ataatcatgg ctttccaggc
    14581 gcaccagttc agctgtgaat tgtgttaagc gaaaaggacc cgtgccaatc agtgggaatt
    14641 gcggatgcgc cagatggctg caatagctcg ccagccggtg cgcaagccag taatcggggc
    14701 gatgtaaaaa gaaggtcaga cactgcggat gggtgacctc aatacgcttc acgctaataa
    14761 ataattgatc cagtgctggc agttgtaaca gcatcaataa tcgctggtgt aagtgtgagg
    14821 cttttactgc atcgccgtta tgccagtgta gggttgaacg aagataaaag tcccagcgta
    14881 acccgtcagc agaggtttcc cagtgatgcg ctaaatcgcc aatcgggcgc tgggtattat
    14941 tatcgaagcg ggtcaggccg gaaaatatct gtccggcgag atgctgctcg gcacggccgg
    15001 gcaaaaagcc cggttgtagc ggttcgagcg ggcgatagta gggaatacgc aacgtgggtg
    15061 tatcgttttg ccattgtccg cccataaacg gctgtaacag agtgcgcagc tcacctgggg
    15121 ccagttgcgc cagctccagc acatcttgct gctttccggt ttccagtgcc ttttccatca
    15181 tcgcattgcg tagcgattct ggcgtgacca gaaagcgtaa ttgtccgcgc tttccgcgtc
    15241 ctgactgcgc ctgccactcc agccatcccg cttcctgtgc ctgacgcaac agcgtacgaa
    15301 catggcgttc gctgcaaaaa cagcgttcgg ccagttcgct gacggtgacg gtttgcggct
    15361 ttccggcgga aggttgccac agacgttgat actggttaag gcggttgagc agtcgcataa
    15421 aaacccggaa caatattatt taactattca ctattacttc cgtatatatc aggtgatact
    15481 caatcaccat taaccgtgtc acagagtgga gaaagaaatg gctcgtctgg cagcatttga
    15541 tatggatggc actttattga tgcctgacca tcatttaggt gagaaaaccc tctctacttt
    15601 ggcgcgactg cgtgaacgcg acattaccct cacttttgcc acggggcgtc atgcgctgga
    15661 gatgcagcat attctcgggg cgatatcgct ggatgcgtat ttgattaccg gcaacggaac
    15721 gcgcgtgcat tctctggaag gtgaactttt acatcgtgat gatttacctg cggatgtcgc
    15781 ggagctggtg ctgtatcagc aatgggatac ccgagccagc atgcatattt tcaatgacga
    15841 cggttggttt accgggaaag agatccctgc gttgttgcag gcatttgtct atagcggttt
    15901 tcgttatcaa ataatcgatg tcaaaaaaat gccactcggc agcgtcacca agatctgctt
    15961 ctgtggcgat cacgacgatc ttacacgttt gcagatccag ctacacgaag cattaggcga
    16021 gcgtgcacat ttgtgttttt ccgccacgga ttgccttgaa gtgctgccgg tgggctgcaa
    16081 taaaggcgct gcactgacgg tgctgaccca acatttaggt ttatcgttgc gcgattgcat
    16141 ggcctttggt gatgcgatga acgatcgcga aatgttaggc agcgtcggta gcggatttat
    16201 tatgggcaat gcgatgccgc aactgcgcgc ggagctcccg catttaccgg tgattggaca
    16261 ttgccgaaat caggctgtct ctcactattt gacgcactgg ctggactatc cacatctacc
    16321 ttattccccc gaataacgag atcccttcca gcaccgggca attgcccggt ttttttgcgt
    16381 tgaatttgtc attttgtgcc gtggtgttta aaccgcacag aataaattgt cgcgatttca
    16441 cctttaaaat agaattaaaa gagaaaaaaa ttctccgtgg aagggctatg ttagataaaa
    16501 ttgaccgtaa gctgctggcc ttactacagc aggattgcac cctctctttg caggcactgg
    16561 ctgaagccgt taatctgaca accaccccct gctggaagcg cctgaaacgg ctggaggacg
    16621 acggtatcct tatcggcaaa gtcgccctgc tggatccgga aaaaataggc ctcggcctga
    16681 ccgcctttgt gctgataaaa acgcaacatc acagcagcga atggtattgc cgctttgtca
    16741 cggtggttac cgaaatgcca gaagtgctgg ggttctggcg catggctggt gaatacgatt
    16801 atctgatgcg cgtccaggtt gccgacatga aacgctacga cgagttttat aagcgtctgg
    16861 taaacagcgt gccggggctg tcggacgtca cttccagctt cgcgatggaa cagattaaat
    16921 acaccacttc tttacccatc gaataaatat ccagaatcag gtcaggacac aacgcgtgcg
    16981 attatttgct caattaagct ggtatttccg tcgggaatgg cgtcgctatc tcggggctgt
    17041 cgccttgctt gtcattatcg cgatgctgca actggttcca ccaaaagtgg ttggtattgt
    17101 tgtcgatggc gtgacagaac aacactttac taccgggcag atcctgatgt ggatcgccac
    17161 catggtgctg attgccgttg tggtttatct cctgcgttac gtctggcgcg tattgctgtt
    17221 tggtgcgtct tatcaactgg ctgttgaact gcgtgaagat tattaccgtc agctaagccg
    17281 gcagcatcct gagttttacc tgcgtcatcg cacgggtgac ctcatggctc gtgcgactaa
    17341 tgacgtcgat cgcgtcgtgt ttgccgccgg agaaggggtg ctgacgctgg tggattcact
    17401 ggtaatgggc tgcgctgtgt tgattatgat gtctacgcaa attagctggc agttgacctt
    17461 atttgccctg ttaccgatgc cagtgatggc gatcatgatt aagcgcaacg gcgatgcttt
    17521 gcatgaacgc tttaagctgg cacaggcggc gttttccagt cttaatgacc gcacccagga
    17581 aagcctcacc agtatccgca tgatcaaagc ctttggtctg gaagatcgcc agtcggcgtt
    17641 atttgccgcg gatgccgaag ataccggcaa aaaaaacatg cgggtggcgc gtattgatgc
    17701 tcgtttcgac ccgaccatct atatcgcgat tggtatggcg aacttgctgg cgattggcgg
    17761 cggtagttgg atggtggtgc agggcagttt aacgctgggc cagctcacca gttttatgat
    17821 gtatttaggt ctgatgattt ggccaatgct ggcgctggca tggatgttta acattgtgga
    17881 acgtggtagt gctgcgtaca gccgtattcg cgcgatgctg gcggaagcgc cggtggtgaa
    17941 cgatggtagc gaacccgtgc cggaagggcg tggcgaactg gatgtaaata ttcaccagtt
    18001 cacgtatccg cagactgacc atcctgcgct ggaaaacgtc aatttcgccc tgaaacccgg
    18061 tcagatgctg ggtatctgcg ggccgactgg ttccggcaaa agtaccctgt tgtcgctcat
    18121 tcagcgtcat ttcgacgtca gcgaggggga tattcgcttt catgatattc ctctgacgaa
    18181 gttacaactc gatagctggc gtagccgcct ggcggtagtt agccagacgc cattcctttt
    18241 ttctgacacc gtggcgaata acatcgcgct gggttgcccg aatgccaccc agcaagagat
    18301 tgagcatgtc gcgcggttag ccagcgtaca tgacgatatt ttgcgtctac cgcaaggtta
    18361 cgatacagag gtgggcgagc gcggtgtgat gctttccggc gggcaaaaac agcgtatctc
    18421 cattgctcgt gcgttattag tcaacgcgga aatcctcatc cttgatgatg cgctttcggc
    18481 ggtggacgga cgcactgagc accagatcct gcataacctg cgtcagtggg ggcagggaag
    18541 aacggtaatc atcagtgccc atcgcctttc tgcactgacg gaagccagtg aaattattgt
    18601 gatgcagcac ggacatatcg cccagcgtgg caatcatgat gtgctggcac aacaaagcgg
    18661 ctggtatcgc gatatgtatc gctatcaaca actggaggcg gcgctcgacg acgctccgga
    18721 aaatcgcgag gaggccgtcg atgcgtagtt ttagccaact gtggccgact ctcaagcgcc
    18781 tgttagcgta cggttcaccg tggcgtaaac cgctggggat tgcggtcctg atgatgtggg
    18841 ttgcggcggc ggcagaagtc agtgggccgc tgcttatcag ctattttatc gacaatatgg
    18901 tagcgaaaaa taacctgccg ttgaaagtgg ttgcagggct ggctgcggcg tatgttgggc
    18961 tgcaactgtt tgccgccggg ctacattacg cgcagtcgct gctgtttaat cgggcggcag
    19021 taggcgtagt gcaacagttg cgtaccgacg tgatggatgc tgcgttacgc cagccattaa
    19081 gcgagtttga tacccaaccc gtcgggcagg tgatttcccg cgtcactaat gacactgaag
    19141 tgatccgcga tctctacgtt accgtagtgg caactgtcct gcgcagtgcc gcactggtgg
    19201 gcgcgatgct ggtggcgatg ttcagcctcg actggcgaat ggcgctggtg gcgataatga
    19261 ttttcccggt ggtgctggtg gtaatggtga tataccagcg ttacagcacg ccaattgtcc
    19321 gtcgtgtgcg cgcctatctg gcggatatca acgacggctt taacgaaatc atcaatggca
    19381 tgagcgttat ccagcagttt cgtcagcagg cgcgatttgg cgaacgtatg ggtgaggcca
    19441 gtcgttcaca ctatatggcg aggatgcaaa ccctgcgcct cgacggtttt ctgctgcgtc
    19501 cgctgctgag tctgttttca tcgctcattc tctgtggctt gttgatgctg tttggcttct
    19561 ccgccagcgg caccattgaa gtgggcgtgt tgtatgcgtt tatcagctat cttgggcgac
    19621 ttaacgaacc cttaatcgaa ctgaccacgc agcaggcgat gctgcaacag gctgttgttg
    19681 ctggtgagcg cgtgtttgag ctgatggacg gaccgcgcca gcaatatggc aatgatgatc
    19741 gcccgttaca gagtggcacc atcgaagtcg ataacgtgtc atttgcttat cgcgatgaca
    19801 atctggtgct aaagaacatt aatctctctg tgccttcgcg taactttgtg gcgctggtcg
    19861 ggcataccgg cagtggtaaa agtaccctcg ccagtttatt gatgggctat tacccgctaa
    19921 cggaaggtga gattcgcctt gatggtcgtc cattaagttc gctaagtcac agcgcgctgc
    19981 gccagggcgt ggcaatggtg cagcaagatc cggtggtgct ggcggatacc ttcctcgcca
    20041 acgtgacgct ggggcgggat atctccgaag aacgcgtctg gcaggcgctg gaaaccgtgc
    20101 aactggcgga gctggcacgc agtatgagcg acgggattta tacgccgctg ggcgagcagg
    20161 ggaataatct ctctgtcggg caaaagcaac tgctggcact ggcgcgcgtg ctggtcgaga
    20221 cgccgcaaat cctgatcctt gatgaggcaa ccgccagcat tgactccggt actgaacagg
    20281 cgattcaaca tgctctggcg gcggtgcgtg aacataccac gctggtagtg attgctcacc
    20341 gcttatcgac tattgttgat gccgacacta ttctggtgct tcatcgtggg caagccgtgg
    20401 agcagggcac tcaccagcaa ctgctggcgg cccagggacg ctactggcag atgtatcaac
    20461 tgcaacttgc gggcgaagag cttgcagcca gcgtgcgtga agaagaatca ttgagcgcct
    20521 gaatagcgca atatctcatc gttggtgcaa aaatgtaacg cactgtgcac tgtcatagtg
    20581 cgttttcatt ttcaatcttc ttaacttcct gctctctttc tcgtttttca tttctggcac
    20641 accgcttgca ataccttctt cgtgtagcag aaccattacc gaattctgac cggaggggat
    20701 ctatgaagct ggtgaccgtg ataatcaaac cattcaagct ggaagacgtt cgtgaagcgt
    20761 tatcttccat tggtattcag ggcctgaccg tcaccgaagt gaaaggtttc gggcgtcaga
    20821 aagggcatgc cgagctgtac cggggggcgg aatacagcgt caacttcctg ccaaaagtaa
    20881 aaattgatgt ggcgattgct gatgaccaac tcgatgaagt gatcgatatc gtcagtaagg
    20941 cggcttacac cggaaaaatt ggcgacggca aaatcttcgt cgctgaattg caacgcgtca
    21001 ttcgtattcg taccggcgaa gccgacgaag cggcgctgta atctctggca cacagcaaca
    21061 ggaacgaaaa atgaagatag cgacgataaa aactgggctt gcttcactgg cgatgctgcc
    21121 gggactggta atggctgcac ctgcggtggc cgataaagcc gacaatgcgt ttatgatgat
    21181 ttgtactgca ctggtgctgt ttatgactat tccggggatt gccctgtttt acggtgggtt
    21241 gattcgcggc aaaaacgtgc tgtcgatgct gacgcaggtg acggtgacgt ttgcactggt
    21301 ctgtattctc tgggtggttt acggttactc gctggcgttt ggtgagggca acaacttctt
    21361 cggcaacatt aactggttga tgctgaaaaa catcgaactg acggcggtga tgggcagcat
    21421 ttatcagtat atccacgtag cgtttcaggg atcgtttgcc tgcattaccg taggcttgat
    21481 agttggggcg ctggcggaac gaatccgctt ctcagctgtg ttgattttcg tggtggtatg
    21541 gctgacgctc tcttacattc ctattgcgca tatggtgtgg ggcggtggtt tgctggcttc
    21601 tcacggtgcg ctggatttcg cgggtggcac ggtggtgcat attaacgccg caatcgccgg
    21661 tctggtgggc gcgtatctga taggcaaacg cgtgggcttc ggtaaagagg cgtttaaacc
    21721 gcacaacctg ccgatggtct tcaccgggac tgccattctc tatatcggtt ggtttggctt
    21781 taacgccggg tcagcgggca cggcgaatga aatcgcggca ctggcatttg tgaatactgt
    21841 ggtcgcaacg gcggcggcaa ttcttggctg gatcttcggt gaatgggcgc tgcgtggtaa
    21901 gccttcactg ctgggagcgt gttctggcgc gattgccggt ctggtcggcg tgacgccagc
    21961 gtgtggctac attggtgttg gcggcgcgtt gattatcggc gtggtagctg gtctggcggg
    22021 cttgtggggc gttaccatgc tcaaacgctt gctgcgggtg gatgatccct gcgatgtctt
    22081 cggtgtgcac ggcgtttgtg gcattgtcgg ctgtatcatg actgggattt ttgccgccag
    22141 ctcgctgggc ggcgtgggct tcgctgaagg tgtgacgatg ggccatcagt tgctggtaca
    22201 gttggaaagc attgccatta cgattgtgtg gtctggtgtg gtggcattta tcggctacaa
    22261 actggcggat ctgacggttg gtctgcgtgt gccggaagaa caagagcgag aagggctgga
    22321 cgtcaacagt cacggcgaga atgcctataa cgcataacaa gcactgcaaa aaacagccgg
    22381 acggttttca cctccggcta tttttttaat tgtgattacg catcacccct tcctgaacgg
    22441 tcgaggcaac cagtacgccg tcctgggtat aaaactcacc gcgcacaaag ccacgtgcgc
    22501 tggacgccga ggtgctctcc acgctataca gcagccattc attcaaatta aacgggcgat
    22561 ggaaccacat ggaatggtca atggtggcaa tctgaatccc cggttcgaga aaaccgatac
    22621 cgtgcggctg tagagctacc ggcaggaagt taagatcaga agcgtaaccg agcagatact
    22681 gatgaacgcg caggtcatcc ggcacgctac catttgcgcg gatccacacc tgacgatgtg
    22741 gttctgcgac gtgacctttc agtgggttat gaaactccac cggacggact tccagcggac
    22801 gatcgcagat gaatttatct ttaagcactg gcggcagcag gtgcgccagc gattgggcga
    22861 tttgcgtttc cgaaggcagg ccatcaggcg ctggcgcgga cggcattgtt ttttgatgtt
    22921 cgaaacccgc ttctggtgcc tggaaagagg ccgtcatata aaaaatcggt ttgccgtttt
    22981 gaatagcagc aacccggcgg gcgctgaagc tgttaccgtc acgcagcgtt tcgacatcat
    23041 aaataatcgg cttcttacta tcgccagggc gaagaaagta gctgtgaaac gaatgtacca
    23101 gccgctcttc agggacggtc tcttttgcag catacaaggc ctgacccacg acctggccgc
    23161 caaatacctg gcgtaaacct aaatcttcac tctggccgcg aaagagtcct tcctcaattt
    23221 tttccagatt taacaatgtc agtaaatttt ttagcgcctg actcatataa ctctccagta
    23281 acaaagctgc cgcagcaagc caaagtgagt tgagtataac gcaaatttgc tactggtccg
    23341 atgggtgcaa tggtctgaat tacgggctaa ttacaggcag aaatgcgtga tgtgtgccac
    23401 aattgttgac gttactattt tgttaaccac tcttccggcg aggaaagtta gcccgctggt
    23461 gcattgataa taaggagaaa tgaatgaaac tcgtgcacat ggccagtggt ttagcggttg
    23521 cgattgcgtt ggcggcttgc gcagataaaa gcgcggatat tcagacgcca gccccggctg
    23581 caaatacgtc tatttcagca acacaacaac ctgctatcca gcaaccgaat gtctccggta
    23641 ccgtctggat ccgtcagaaa gtcgcactgc cgcctgatgc tgtgctgacc gtgacacttt
    23701 ctgacgcgtc gttagccgat gcaccgtcaa aagtgctggc gcagaaagcg gtgcgtactg
    23761 aaggtaaaca gtcaccattc agctttgttc tgccatttaa cccggcagat gttcagccga
    23821 acgcgcgtat tctgttgagt gcggcgatta ccgtgaatga caaactggta tttattaccg
    23881 ataccgttca gccggtgatc aaccaggggg gaaccaaagc cgacctgaca ttggttccgg
    23941 ttcagcaaac cgccgtgcct gtccaggcca gtggtggcgc aacaactacc gtaccatcga
    24001 cttcaccgac ccaggtgaat ccatcttcgg cagttcctgc ccctacgcaa tactaagcgt
    24061 tactaacccc tttccatctg gagaggggtt agtagttcca gcgatagcgc tgtaagtcga
    24121 tttgcccgct tcccgatacc atcacacctt ctgccagtaa tgcctgtcgt tgacgctgta
    24181 aatccggtcc ggttagcgaa atggtgccgt ggcgattaac cacccggtgc cagggcaagg
    24241 tgctgccttc ggggagacgc tttaacacac cgccaacctg gcgcgcggcg cggggcgatc
    24301 ccgccagttt cgccacatca ccgtaagtgg tgacataccc ttcgggaata gcggctacga
    24361 tttgccagac gcgttgggga aatgaatctt ctttttccat cttttcttcc tgagataatt
    24421 tttcagcata atctggaaaa acgcccgagt gaagccgcat tgcgcaagaa accagcatct
    24481 ggcacgcaat gggttgcaat tagccggggc agcagtgata atgcgcctgc gcgttggttc
    24541 tcaacgctct caatgggggc tctgttggtt ctcccgcaac gctactctgt ttaccaggtc
    24601 aggtccggaa ggaagcagcc aaggcagatg acgcgtgtgc cgggatgtag ctggcagggc
    24661 ccccacccat ttctgcctcc caccgtttcg tcaaaaaatc ccaacatgac taaactttaa
    24721 ccacaactga cgtcgcaaga attgtctggc tgcgcagtac gcttcggagg tatgtctgat
    24781 gaagtatgtt gatggttttg tggttgccgt tcctgccgat aaaaaggatg cctatcggga
    24841 aatggccgct aaggccgcgc cattgtttaa agagtttggc gcgcttcgta ttgtcgaatg
    24901 ctgggccagc gatgtaccgg atggcaaagt gaccgatttt cgtatggcgg tgaaagcgga
    24961 agagaatgaa gaggttgtct ttagctggat tgaataccct tcaaaagagg tccgcgacgc
    25021 agctaatcaa aagatgatgt cggacccacg gatgaaagag ttcggcgagt ccatgccgtt
    25081 tgacggcaag cgaatgatct atggcggatt cgagtcaatc atcgacgaat aggtagcgtg
    25141 acgggctgtg cggcgtgccg tcagcccaaa aattcacaaa tgttgctcgg cccagcgtaa
    25201 gaaatcttct ttcggtaatg ccttgctgta gagccagccc tgaccgtagt gcacgccatg
    25261 ctggcgtagc cactcttctt gtttactggt ttcgattccc tccgctacca ttttcagttt
    25321 cagtgttttt gccatttcga tgatatgcgg cgtgacattt ttatattcca gcgcatcaac
    25381 gaaagattta tcgatcttca gaatgtcgac atccagatcc tgtaaataac ttaaacttga
    25441 ataccccgta ccaaaatcat caagataaat ttcatggccc gcctcccggt agcgagaaat
    25501 tatcggggcg ctggttttcg gatcggcaaa ctcgcgttca gtgagttcaa gcgcgatctg
    25561 tctgggatta acctgatagt gattgatcat ttcacgcagc aattgcggga ttttttccga
    25621 ggtgagcacg gtggattcaa gattgatcga aatatgctgc tgtggatgct gacgcagcca
    25681 gtcgcccata tcttcaaaga cgcttcttat aatcagtagc gtcaatggct cagaaaggcc
    25741 cgtttgctgt gccagcggaa taaaactatc tggtgacaac caactaccgt ctgtctgcgg
    25801 ccagcgcgcc agtgcctcag caccgacaat tttgccattg gctaaggaga caatcggctg
    25861 atagtgcacg caaatatcac gattttcgat agcatcctgc agccgatgat gcggtgactg
    25921 gatgcggcgc aaaatgcgca gcacaaacat cgctgccagc aggccaatca ccaacccgag
    25981 cggtaaccaa ataaagactt gccgatgcca gcctttctgc aacattttcg atgaagccca
    26041 ggtgataatt gaaatattca tctccgggaa gggcaggata tcgtaaatga ttccattatt
    26101 ttcgatatgc tcaccgggtg ttttttgtag cctggtaata attccctgag caatttcatc
    26161 gctgctggtt atgacaacgt tatgggcatt gccaataatg gcggcatcaa tttgccatga
    26221 gctataggga atgacatcaa taaaggaagc ggggtcgatc atgacgacat aatgtgctgt
    26281 tcccatggcg accatgtaac ggataatgcc taaatcgtta tgtgatgtta accagacacg
    26341 ataaccatct ttcgaaattt tacctggctc ggggaaggta tcgggcggac tttcatgctc
    26401 cagagacgaa cactggggaa cgttgttatc gatataaacc acttcctgaa tatagcgata
    26461 actgtaagat acccgacgca tttccatgag atgggcttca ctacagtcag cgccttgcca
    26521 tctttccagc tcttgcagcg catctttccc ttgtgtcgcc accttattgg cccgaatagc
    26581 gacgcgggag gaataggtat ccagctcttc aataaacgat gtttctacct gctgatgggc
    26641 cagccagatg cttaagccga caggcagcaa tactgaaaga atcagtactc ccgaaataag
    26701 gccgaccaga tgtcgtgttc tcaccagcat atccttatta acacagtgta gggaaagtat
    26761 atctgattgt ggaaaaaggc tggcaggaga taaggaggta gagctggtat ttcatgaagt
    26821 gatgggtgga aacgatcgtt gtcatttgac caggcgcaga gctttacgcc tggtatgtaa
    26881 tcctaactta agatgttgat tcggcgcgag cgagggtaaa aatatcgtaa aaagacaatt
    26941 cacctttacg gacgatcatt ttttgcagtt gttccttatg aacatccgcg acacgcggag
    27001 agttcaagat ggcttcaacc agcgcggcag gtacaaagcg cgtgttgtac cattcgccgt
    27061 tatagcaaac gcggaaatcc aacacgtcga tatcttcata gcggtagcgt gggtagacat
    27121 cgaaaaagaa gaaaccatta agcaggataa ataatacaac cagtagccag acagagcctg
    27181 aaagcgtttc ggactgcaac atcaccgcca gggtggcaaa aaaagcaacg tacataccga
    27241 taaacagccc cggatgttta cggataaaac tgatactaaa gcggggtttg ttgtcgcgtt
    27301 tttcgcgggt attcagagac tcaatcgttt cggtcagcag gcgttgtatt tcggtcatcg
    27361 tttgccttcg tggtatctgc aactttccag ggaacaccct attttaacgt gggaatagta
    27421 gagggaaagt aacagatctg ccacaatttt gcataacagt tgcgaaaaac cgattacaat
    27481 tttttaatta ttctggctgg attaccgccc acgacaacgt tgtccgggac atcttttgtg
    27541 acaactgcac ctgaggctac cacgacgtta tcaccaatgg tcacaccagg gttaatgacc
    27601 gcgcgtccgc caatccagac gttattaccg atggtgacgg gtttccccag ttcagcaccg
    27661 ctattacgtg ctacagggtc gatgggatgt gttgccgtgt agatatgaac gcctagtgcc
    27721 aacatacagt tatcaccgat gcgaataggg cagacatcaa gcatcacgca atcgaagttg
    27781 gcgaaaaaat tattaccgag aaaaatgtta tagccatagt cacagcgaaa cgttggctca
    27841 atataagcct ctgtcacctg accgaataga tcagcgagaa tttgctggcg taatgtgtgc
    27901 tcttccgcca gggaatgatt gtatcggtga ataagctgac gagcgcgcag gcgatcgcga
    27961 gataacgtct catctgccga gcgatacaac tcaccagcaa tcatcttttc tttttctgtg
    28021 ctcattactg acctccggtt gctgaaaatg cggcaacaca gtaagacgaa aagtcagtga
    28081 agagaacgtt cccattacat ctgtgtgata aagatcacat agggtatgtc ttcatggcga
    28141 aaaagtataa aattcttaat aaacagccgg ttatagctcc gaaagcgaat tatttattag
    28201 cgaataaatt tccatactga ggaagggatc ttgtcgtaca gtttattcat ggtcaattcg
    28261 gcgaggcggt gatctgcggc tgagtaaaat accgccagtt cattatctga taattcgtat
    28321 ttatttttct cgataacacg ctccagcgtg tcaattgtct ggcaacgacg taaacgcatt
    28381 aaataatcgg ttttcgttaa aggtttttcg gacatacttc tacccatggt tgtaataatt
    28441 ctaacaagat aaactcgcag gattctcttt cgtcgcattg acaaaacaac ggaatagtcg
    28501 attacctgac ttccgccatt tctgaagatc ctgcatatta ataccataac tactgaacaa
    28561 cataaaggtg tcatccagat attcgtcgat ctgctcaatg agcttattgt cttcattata
    28621 cttaattttg taattaagtg cgaaggtcgc aatatgctca atcagttcat tcaactggag
    28681 gttgatcgcc gaggttgggt cgttaaccca gccatgattg ctttcttcaa ggtttgcaag
    28741 gcagtcatga tacagagttt cacagagaaa cttaagctgt gcgatatcat gtcttttggg
    28801 tgagtattca tccataacgc gtccccttct tagcggttga actaacggac acctttcggg
    28861 atggaaaaaa cttactgacc tggacttgcc ctctcgtttg ttagcttaac tataagccac
    28921 tctttgcagg ttttcatcgc attattacga aaaattacaa ttaggaagga agcgggacag
    28981 ggctcacttt ttcatactag caccaacccg cagcaggtaa aagcagtttg ctcgaactgt
    29041 tcataacatt tcaggattat cgccagatga aattggtgac actaatacca ggattgctct
    29101 gaatatgacg taataaccga ggaatgaata aagaattacc gcaaataatt aagaatagcc
    29161 tctaaatgat tatggattat taaatctatt agcgctactt aatataaatt aactaacaat
    29221 cagtattttt tatgaatttt atacgtggtt aatactggtt ttcgtatgag atcctgagtt
    29281 ggtggttcaa ttactcctta atgttcgtag gttatgcata aaaaaggccg cttacgcggc
    29341 cttagtgatt acacgttgta tcaatgatga tcgacagtat ggttgtgctc gatatcttca
    29401 ttcttgcggc taaagcggcg gcgaaccacc acaaagaata ccggaacgaa gaagattgcc
    29461 agtaccgttg cggtcaccat cccgcccatt acaccggtac ctactgcgtt ctgcgcgccg
    29521 gaaccagcac cagtactgat aaccagcggc ataacgccga ggataaacgc cagcgaggtc
    29581 atcaggatcg gacgtaaacg catccgcacc gcatcaagcg tcgcttcaat cagaccttta
    29641 ccttctttat ccatcaagtc tttggcgaat tcgacgataa gtatcgcgtt cttcgccgac
    29701 aacccaatgg ttgtgagcag gcctacctgg aagtaaacgt cattggtcag gccacggaag
    29761 gtggcagcca gcaacgcacc gataaccccc agcggaacga ccagcataac ggagaacgga
    29821 atcgaccagc tctcgtacag cgccgccaga cacaggaaca cgacaatcaa cgaaatcgcg
    29881 tacagtgaag gtgcctggtt gccggagaga cgttcctgat aggacatccc cgtccagtca
    29941 tagccaacac cggtaggcag tttgctcgcc agttgttcca tcagctccat tgcttcaccg
    30001 gtacttttac ccggtgccgc ctggcctaag atttccatgg atggcaggcc gttgtaacgt
    30061 tccagacgcg gcgaaccgta ctcccaacga gaagaggaga acgccgagaa tggcaccatc
    30121 tgaccatcag cagcacgaac ataccagtcg ccgatatcat ccggcagcat acggtatttc
    30181 gcttctgaca tgacgtaaac tttcttcaca cgaccgcggt cgataaagtc gttcacatag
    30241 ctgccgcccc atgcagcgcc cagagtggtg ttaatgtcgt tgatagaaac acccagcgcc
    30301 tgcgcttttt cctggtcgat atcaatctta aactgcgggg tatcttccag accgtttgga
    30361 cgtacgctgg tcaacatatc agggtgcttc gctgcttctg caagcaactg gttacgcgcc
    30421 tgagtcagtt tttcgtgacc aaggccagcc tggtcaatca gctcaaagtc aaagccggtt
    30481 gcggtaccca gttccacgat tgcgggcagg ttaaaggcga aaaccatcgc atctttgatt
    30541 tgcgagaaag cgcgtgttgc acgcatggta atcgcttcaa ctttgttttc ttcacccgga
    30601 cgatcggccc agtccttcaa ggaaacgaac gcaataccgg tattctgacc acgtcccgca
    30661 aagccgaagc cgttaacggc gaacaccgac tcaacgttgt tcttttcttt ggtcagatag
    30721 taatgcgtta cctcattgag cactttctgc gtacgttcct gcgttgcacc tgctggcagc
    30781 tgaaccatgg tcataaatac gccctggtcc tcatctggca agaaggagct tggcagacgc
    30841 acaaacagat aggccatgcc gaccacgatg atcagataca gcaccagata acgccccgta
    30901 ctgcgcagaa taccgcctac gctgtcggtg tagtggtgcg tgctcttctc gaacatgcgg
    30961 ttaaaccagc cgaagaagcc ttttttacct tccccgtgat cgcctttggc aatcggtttc
    31021 agcatggtgg cacaaagagc tggagtcagg atcaacgcca ccagtaccga cagcgccatt
    31081 gctgaaacaa tggtaataga gaactgacga taaattgccc cagtagaacc gccaaagaag
    31141 gccatcggta cgaataccgc cgacagtacc atcgcgatac cgaccagagc gccctgaatc
    31201 tgccccatcg acttacgggt ggcttctttt ggcggcaaac cttcttccgc catcacacgc
    31261 tcaacgtttt ctaccacaac gatagcgtca tccaccaaca ggccgatggc gagcaccatc
    31321 ccgaacattg ttagcgtgtt tatcgagaag ccaaaggcgg caaggacggc aaaggtcccg
    31381 agcaatacca ccggtacggc aatggtcgga atcaacgtcg cgcggaagtt ctgcaggaac
    31441 agatacataa ccaggaacac gaggatgatc gcttcgacca gcgttttaac cacttcgtga
    31501 atagagattt tcacgaacgg cgtggtgtcg tacgggtaaa caattttcag acccgacggg
    31561 aagaacggtt ccatcttcgc cagttcagca cggattgccg cagcggtatc cagcgcgttt
    31621 gcaccggtcg ccagcttgat ccccagaccg gaagccggtt ggccgttaaa ctctgcgatg
    31681 atgtcgtagt tctcaccacc cagctcaatc ttcgccacat cacgcagcag tacgcgggaa
    31741 ccatcctgat tcactttcag caggattttg ccgaactctt cagtagaggt cagacgcgtc
    31801 tgagcaataa tagaggcgtt aagctgttgg cctttcaccg gcggtgtacc accgagctgg
    31861 cccgccgcaa cctgggcgtt ctgcgctttg atggcggtaa taacatcaac cggcgttagc
    31921 tggaatttgt tcagttcatt cgggttcatc cagatacgca tcgcgtactg tgaaccgaac
    31981 aactgaacgt cacccacgcc agacgtacgg ctgatggcat ctttcatatt cgccgccacg
    32041 tagtcggaga tatcctcctg cgtcatggtg ccatcggtgt tgataacgcc gacaaccatc
    32101 aggaagctgc tggatgattt ctcaatgctc accccttgct gctgaacttc ttgcggcagc
    32161 aacggcatcg ccagctgcag tttgttctgc acctgaacct gcgcgatatc cgcatcagta
    32221 ccagactcaa aggtcagggt gatctgtacg gtacccgtgg agtcactgtt agaggacatg
    32281 tacatcaggt tatcgatacc gttcatattc tgttcgataa cctgtgtcac cgtgtcctgc
    32341 actgttttcg catcagcgcc ggggtaggag gcggagatcg ttactgccgg cggtgcaatc
    32401 gtaggatatt gcgccaccgg cagtttgagg atcgccagcc cccctgccaa catgatgata
    32461 atggcgatca cccacgcaaa aatcgggcga tcgataaaga aattaggcat gtcttaacgg
    32521 ctcctgttta agttaagact tggactgttc aggctgagca ccgcttgcgg cttgctggtt
    32581 attatcagcg gtaacttctt gtgcttttac ctggacacca ggacgcactt tctgcagccc
    32641 acttattact acgcgatcgc ctgctttcag accttctgtc accagccact tatcgccaat
    32701 agcctggctt gcaacgatcg gacgggtttc cactttgtca tccgcgccaa ccaccagtac
    32761 ggtggcatcg ccacgcggcg tacgggttac gccctgttgc gggactaaaa tagcgtttgg
    32821 attaagccct tcttccagac gtgcgcgcac gaacataccc ggcagcagag tgtgatccgg
    32881 gttcgggaag atagcgcgta gggtgataga cccagtggtc tgatcaacgg taacgtcaga
    32941 gaattccagc gtaccgtcct gcgggaactt aatgccgtca ctggtgatca gcgacacttt
    33001 ggctttgccg ttctcttgtt tcagcgtgcc attcgccagt tcctgtttca ggcgcaggaa
    33061 gtcgttgctg gactgggtca catcaacgta gatcggatca agttgctgca cggttgccag
    33121 cgcagtcgcc tgaccgttct gtaccaatgc gccttccgtc acgttcgact taccaatgcg
    33181 accgctaatc ggagaggtga ctttggtgta agccagattg atccgcgcag tttcaacggc
    33241 agctttcgcc gcagttaccg cagcattcgc ctgttgcgca tcagccagag cctgatcgta
    33301 ctcttgctta ctgatgtact gagtaccgag cagtttctga taacgattca ccgtcaattg
    33361 cgcgatattg gctgcagcct gggctttcgc cagatcacct ttcgcactgt cgtatgtcgc
    33421 ctgataggtc gcaggatcaa tctgatagag agagacacct gcttcgatgt cgctaccttc
    33481 tttgaaatta cgcttcagga taatcccgct aacttgagga cgaacttctg cgatccggta
    33541 ggcactggtg cgacccggaa gctcggttgt gatctgcaga ggttcagttt tgactgttac
    33601 tacgccaacg gcgggcatct gctggccacc ttgttgggcc tgtttgtcgt cacatcctgt
    33661 tagggctaag ctgcctgaga gcatcagaac gaccgccaga ggcgtaaacc ctctgttttt
    33721 gttcatatgt aaacctcgag tgtccgattt caaattggtc aatggtcaaa agttaataaa
    33781 cccattgctg cgtttatatt atcgtcgtgc tatggtacat acattcacaa atgtatgtaa
    33841 atctaacgcc tgtaaattca cgaacatatg gcacgaaaaa ccaaacaaga agcgcaagaa
    33901 acgcgccaac acatcctcga tgtggctcta cgtcttttct cacagcaggg ggtatcatcc
    33961 acctcgctgg gtgagattgc aaaagcagct ggcgttacgc gcggtgcaat ctactggcat
    34021 tttaaagaca agtcggattt gttcagtgag atctgggaac tatcagaatc caatattggt
    34081 gaactagagc ttgagtatca ggcaaaattc cctagcgatc cactctcagt attaagagag
    34141 atattaattc atgttcttga atccacggtg acagaagaac ggcgtcgatt attgatggag
    34201 attatattcc acaaatgcga atttgtcgga gaaatggctg ttgtgcaaca ggcacaacgt
    34261 aatctctgtt tggaaagtta tgaccgtata gaacaaacgt taaaacattg tattgaagcg
    34321 aaaatgttgc ctgcggattt aatgacgcgt cgggcagcaa ttattatgcg cggctatatt
    34381 tccggcctga tggaaaactg gctctttgcc ccgcaatctt ttgatcttaa aaaagaagcc
    34441 cgcgattacg tcgccatctt actggagatg tatctcctgt gccccacgct tcgtaatcct
    34501 gccactaacg aataaccctg aatctgactc caggattttt cctggacatt ttcgtcgttg
    34561 ctattctggt tcactgcgtc gtgatattct tgcggtttga ctttttcagg tcgttcttca
    34621 ggttcagaaa ccttcattca tcatgactat gttccagtat tacaaacgat cacggcattt
    34681 tgttttttca gcatttattg cttttgtttt tgtcttgtta tgccagaaca cggcgtttgc
    34741 gcgggcgtca tcgaatggtg atctgccgac aaaagcggac ctgcaggcgc aacttgactc
    34801 actaaataaa caaaaagatc tttctgctca ggacaaactg gtgcagcagg atctgacaga
    34861 tacattagcc accctcgata aaatcgatcg cgtaaaagaa gagaccgttc agctacggca
    34921 aaaagtcgct gaagcgccgg aaaaaatgcg ccaggcgacc gcggcgttaa cagcacttag
    34981 cgatgtcgat aacgacgaag aaacgcgcaa aattctgagc acgctttcgt tgcgccagct
    35041 ggaaactcgc gttgcccagg cgctggacga tttgcaaaac gcacaaaacg atctggcgtc
    35101 ttataacagc cagctggttt cgttacagac gcagcccgaa cgcgtgcaaa atgcgatgta
    35161 taacgcttcg cagcagctgc aacaaattcg cagtcgtctg gatgggactg atgtcggcga
    35221 gacggcctta cgtcccagcc agaaagtgtt aatgcaggct cagcaggcgt tgctgaatgc
    35281 ggagattgac cagcagcgta aaagcctgga agggaatacc gtcttgcagg ataccttgca
    35341 aaagcaacgt gattacgtga cggcgaacag cgcccgtctg gagcaccagt tgcagctgtt
    35401 gcaagaagcg gtaaacagca agcgcctgac tttaaccgaa aaaacggcgc aggaagccgt
    35461 ctccccggat gaagccgcgc gtattcaggc taatccgctg gtgaagcagg aactggaaat
    35521 taaccagcag ttaagtcagc gtctgattac cgcgaccgaa aacggtaatc agttgatgca
    35581 gcaaaacatt aaagtcaaaa actggctgga gcgggcgctg caatcggaac gcaatattaa
    35641 agagcagatt gccgtcctga agggcagcct gctgttgtct cgtatccttt accagcaaca
    35701 acaaacgctg ccctcggcgg atgaactgga aaacatgacc aaccgcatcg cggatttgcg
    35761 tctcgaacag tttgaagtta accagcagcg tgatgcactc ttccagagcg atgcgttcgt
    35821 caacaaactg gaagaaggtc acaccaacga agtcaacagc gaagttcacg atgcgttatt
    35881 gcaagtggtt gatatgcgtc gcgaattgct ggatcaactc aacaaacagt tgggtaacca
    35941 gctgatgatg gccattaacc tgcaaatcaa ccagcagcag ttaatgagtg tgtcgaaaaa
    36001 cctgaaatcc atcctgactc agcaaatctt ttgggtgaac agtaaccgtc caatggactg
    36061 ggactggatc aaagcgttcc cgcaaagcct gaaagatgaa tttaagtcga tgaaaattac
    36121 ggttaactgg gaaaaagcct ggcccgccgt ttttatcgct ttcctcgctg gtttgccgct
    36181 gctgttgatt gccgggctga tccactggcg tctggggtgg ctgaaagcgt atcaacaaaa
    36241 actggcttcc gctgtgggtt ccctgcgtaa cgacagccag ctcaacacac caaaagcgat
    36301 ccttatcgac ctgatccgtg cactgccggt gtgcctgatt attctcgcgg ttggcctgat
    36361 tctgttgacc atgcagctca acatcagcga actgctatgg tcgttcagca aaaaactggc
    36421 gattttctgg ctggtgtttg gcctgtgctg gaaagtgctg gagaagaacg gcgttgccgt
    36481 gcgtcacttc ggcatgccgg aacagcagac cagccactgg cgtcggcaaa ttgtccgcat
    36541 cagtctcgcg ttgctgccta tccatttctg gtctgtggtg gcagaacttt ccccgctgca
    36601 tctgatggat gatgtgctgg ggcaggcgat gattttcttc aacctgctgc tgattgcctt
    36661 cctggtatgg ccaatgtgcc gcgaaagctg gcgtgataaa gagtcgcaca ccatgcgact
    36721 ggtcaccatt accgtgctgt cgattatccc gattgcattg atggtgctga ctgccacggg
    36781 ttatttctac accacgctgc gcctctccgg gcgttggatt gaaaccgttt atctggtgat
    36841 catctggaac ttgctctacc agacggtatt gcgcggctta agcgtagcgg cgcggcgtat
    36901 cgcctggcgt cgtgcgctgg cgcgtcggca gaatctggtg aaagagggcg cagaaggtgc
    36961 cgaaccgccg gaagaaccca ccattgcact ggaacaggtt aaccagcaga cgctgcgtat
    37021 taccatgttg ctgatgtttg cgctgttcgg tgtcatgttc tgggcaattt ggtccgattt
    37081 gatcaccgtg ttcagctatc tcgacagcat tacgctgtgg cattacaacg gtactgaagc
    37141 tggtgctgcg gtggtgaaaa acgtcaccat gggtagtctg ttgtttgcga ttatcgcctc
    37201 aatggtggcc tgggcgttga ttcgcaacct gcctggtttg ctggaagtgc tggtgctctc
    37261 gcgactgaat atgcgccagg gcgcgtcgta tgccattact accatcctta actacatcat
    37321 tattgctgtt ggtgcgatga cggtgttcgg atcgctgggc gtctcttggg ataaactcca
    37381 gtggctggcc gcagcattat ccgtaggtct tggttttggt ttacaagaaa ttttcggtaa
    37441 cttcgtctcc ggtttgatca ttctgttcga acgtccggtg cgtattggcg atacggtaac
    37501 cattggtagc ttctcgggga cggtaagtaa aatccgtatt cgtgcgacaa cgattaccga
    37561 tttcgatcgc aaagaagtga tcatcccgaa caaagcgttt gttaccgagc gtctgatcaa
    37621 ctggtcgttg actgacacta ctacgcgtct ggtgatccgt ctcggcgtgg cctatggctc
    37681 cgatctggaa aaagtgcgta aagtgttact gaaggcggcg actgagcacc caagggtgat
    37741 gcacgaacca atgccggaag tcttctttac ggcatttggt gccagcacgt tggatcatga
    37801 gctgcgtctg tatgtgcgtg aactgcgtga ccgtagtcgt actgtcgatg agctgaaccg
    37861 tactatcgat cagctgtgcc gtgaaaacga catcaacatt gcctttaacc agcttgaagt
    37921 gcatctgcac aacgagaagg gcgatgaggt gacggaagta aaacgcgact acaaaggcga
    37981 cgatccgacg ccagcggtag ggtaaggaca ataaagggcg acagtgatgc cgccctttat
    38041 tttttactcg ctttgtgaag cctcatcgct ctgtaacttc ttttcataat cacgctttac
    38101 aatatccagc gcgcgcagca cggtgcgggc tgggatctga ttttcttcca gcagcacaat
    38161 caaatcgaca gccagtttga catcgtcagg ggcattttcc agtgacatac tctctccatt
    38221 gctagcgggt taaacgcgct aacctgtttt cgattttttc cagcgcatgg cggcagcgtg
    38281 ccaggcgcgc ttcataggct tccacttcac gatgcagcgt ttgctgttcc acgagatcgg
    38341 tcacccgcgc caggcgggct ctacgttcgg caaccatctc acgtagccgc cgctcaaaat
    38401 cctgatgctg aatacgttta cgctgccagc gggcaatttg gggtggtgcg ctatcccact
    38461 cgcgtagcga ccaggctgtg gcttcacgcg ctatagcctc cagttgcgcc gccagatgtt
    38521 ctgccagcca ggcgacttgc ggcagttgct gttgctcaac tgcatgacga agcgcagcca
    38581 gattatcgcc cgccttgtcg agacaagcct gtagtgttgt cgcacgagtc tgaaaaagat
    38641 gcctgttgaa acgagcactt agcgtggcga actgtgacac cggggcacaa cgctgacgca
    38701 gcgtagcgag ctgtccttcc agtttttcca gcagcagggc ggttttcacg ataagacctc
    38761 aatgaaaatg ataattgtta tgctaaagta gccactctgt aagctgacaa cactcaatat
    38821 gcaacgaatc attttaatca tcattggctg gctggcggta gtgctgggca cgctgggcgt
    38881 ggtattaccg gtattaccga cgacgccgtt tatcctgctg gcggcctggt gctttgcccg
    38941 ttcttccccg cgctttcacg cctggttgct gtaccgctca tggtttggca gctatctacg
    39001 tttctggcag aaacatcatg cgatgccgcg cggcgtcaaa ccgcgggcga ttttgcttat
    39061 tttgctcacg tttgccattt ctctgtggtt cgtccagatg ccatgggtgc gcatcatgtt
    39121 gctggtaatt ctcgcctgtt tgcttttcta tatgtggcga attccggtga ttgatgaaaa
    39181 gcaagaaaag cactgaagca caacaatcgc agttgcaatt attgcgtaca gccagtacat
    39241 tctggcgttt tcgagcacag gcgcaggcgg tcaaaggtta aacaactgtt acttttgata
    39301 cgtttaaaac gcgccgtgag taccaccgta acaagcaggc atacacttat gaccgcgact
    39361 gcacagcagc ttgagtatct caaaaatagc atcaaaagca ttcaggacta cccaaaaccc
    39421 ggcattcttt tccgcgatgt caccagctta ctggaagacc cgaaagctta cgctctcagc
    39481 atcgacttgc tggttgagcg ttacaaaaat gcgggcatta acaaagttgt cggcaccgaa
    39541 gcgcgtggct tcttgtttgg cgctccggta gctctgggtc tgggcgttgg ctttgtaccg
    39601 gtccgtaaac cgggcaaact gccgcgtgaa accatcagtg aaacttacga tctggaatac
    39661 ggcaccgatc agctggagat ccacgttgat gccatcaaac cgggcgacaa agttctggtg
    39721 gtggacgatc tgctggcaac cggcggcact atcgaagcga ccgttaaact gatccgtcgt
    39781 ctgggtggtg aagtggctga cgctgcgttc attatcaacc tgttcgatct cggcggcgaa
    39841 cagcgtctcg aaaaacaggg cattaccagc tacagccttg tcccgttccc gggccattaa
    39901 ttatcgccag tcttgtgctg cccacgctac ggacagcaca agatgtgcat tcagcctcgc
    39961 cgttctgacg gggctgtgtt agcattaccc cttcgagaat ccaccttcca gcgtttcaga
    40021 gcctgccaat gagttatcag gtcttagccc gaaaatggcg cccacaaacc tttgctgacg
    40081 tcgtcggcca ggaacatgtg ctgaccgcac tggcgaacgg cttgtcgtta gggcgtattc
    40141 atcatgctta tcttttttcc ggcacccgtg gcgtcggaaa aacctctatc gcccgactgc
    40201 tggcgaaggg gctaaactgc gaaaccggca ttaccgcgac gccgtgcggc gtgtgcgata
    40261 actgtcgtga aatcgagcag gggcgctttg tcgatctgat tgaaatcgac gccgcctcgc
    40321 gcaccaaagt tgaagatacc cgcgacctgc tggataacgt ccagtacgct ccggcgcgtg
    40381 gtcgtttcaa agtttatctc atcgacgaag tgcatatgct gtcgcgccac agctttaacg
    40441 cactgttaaa aacccttgaa gagccgccgg agcacgttaa gtttctgctg gcgacgaccg
    40501 atccacagaa attgccggtg acgattttgt cgcgctgttt gcaatttcat ctcaaggcgc
    40561 tggatgtcga gcaaattcgt catcaacttg agcacatcct caatgaagaa catatcgctc
    40621 acgagccgcg ggcgctgcaa ttgctggcgc gcgccgctga aggcagcctg cgagatgcct
    40681 taagtctgac cgaccaggcg attgccagcg gtgacggcca ggtttcaacc caggcggtca
    40741 gtgcgatgct gggtacgctt gacgacgatc aggcgctgtc gctggttgaa gcgatggtcg
    40801 aggccaacgg cgagcgcgta atggcgctga ttaatgaagc cgctgcccgt ggtatcgagt
    40861 gggaagcgtt gctggtggaa atgctcggcc tgttgcatcg tattgcgatg gtacaacttt
    40921 cgcctgctgc acttggcaac gacatggccg ccatcgagct gcggatgcgt gaactggcgc
    40981 gcaccatacc gccgacggat attcagcttt actatcagac gctgttgatt ggtcgcaaag
    41041 aattaccgta tgcgccggac cgccgcatgg gcgttgagat gacgctgctg cgcgcgctgg
    41101 cgttccatcc gcgtatgccg ctgcctgagc cagaagtgcc acgccagtcc tttgcacccg
    41161 tcgcgccaac ggcagtaatg acgccaaccc aggtgccgcc gcaaccgcaa tcagcgccgc
    41221 agcaggcacc gactgtaccg ctcccggaaa ccaccagcca ggtgctggcg gcgcgccagc
    41281 agttgcagcg cgtgcaggga gcaaccaaag caaaaaagag tgaaccggca gccgctaccc
    41341 gcgcgcggcc ggtgaataac gctgcgctgg aaagactggc ttcggtcacc gatcgcgttc
    41401 aggcgcgtcc ggtgccatcg gcgctggaaa aagcgccagc taaaaaagaa gcgtatcgct
    41461 ggaaggcgac cactccagtg atgcagcaaa aagaagtggt cgccacaccg aaggcgctga
    41521 aaaaagcgct ggaacatgaa aaaacgccgg aactggcggc gaagctggcg gcagaagcca
    41581 ttgagcgcga cgcgtgggcg gctcaggtga gtcaactttc gctaccaaaa ctggtcgaac
    41641 aggtggcgtt aaatgcctgg aaagaggaga gcgacaacgc agtatgtctg catttgcgct
    41701 cctctcagcg gcatttgaac aaccgcggtg cacagcaaaa actggctgaa gcgttgagca
    41761 tgttaaaagg ttcaacggtt gaactgacta tagttgaaga tgataatccc gcggtgcgta
    41821 cgccgctgga gtggcgtcag gcgatatacg aagaaaaact tgcgcaggcg cgcgagtcca
    41881 ttattgcgga taataatatt cagaccctgc gtcggttctt cgatgcggag ctggatgaag
    41941 acagtatccg ccccatttga tcgtaagcac agcttacgtt cgtcatcctt aacgtgattg
    42001 agagagaaac ctatgtttgg taaaggcggt ctgggtaacc tgatgaagca agcccagcag
    42061 atgcaagaaa aaatgcagaa aatgcaggaa gagatcgcgc agctggaagt taccggcgaa
    42121 tctggcgcag gtctggtaaa agtgaccatc aacggtgcac ataactgccg tcgcgtagag
    42181 atcgacccga gcctgctgga agacgacaaa gagatgctgg aagacctggt ggctgcagca
    42241 ttcaacgacg cagcacgtcg tattgaagaa acgcagaaag aaaaaatggc ttctgtatcc
    42301 tccggaatgc agctgccgcc tggctttaag atgccgttct gatgcaaacc agcccgctgt
    42361 taacacagct tatggaagca ctgcgctgtc tgccgggcgt tggcccgaag tcggcgcagc
    42421 gtatggcgtt cacgctgctt cagcgcgatc gtagcggcgg gatgcgtctg gcgcaggcgc
    42481 tcacccgggc gatgtcggaa atcggccact gcgccgattg ccgcaccttc accgaacagg
    42541 aagtctgtaa catctgttcg aatccgcgtc gtcaggaaaa cggtcaaatc tgcgtggtgg
    42601 agagtccggc ggacatttac gccattgagc agacggggca gttttcaggt cgttattttg
    42661 tgttgatggg acatctgtca ccgctggacg gcatcggtcc ggatgatatc ggccttgatc
    42721 gtctggaaca gcgtctggca gaggaaaaaa tcactgaagt gatcctcgcc accaacccta
    42781 cggttgaagg tgaagctacc gctaactaca ttgccgagct ttgcgcgcaa tatgacgtgg
    42841 aagccagccg aatcgctcat ggcgtaccgg ttggcggcga actggaaatg gtcgatggca
    42901 ccacgctgtc acactccctt gccgggcgtc ataagattcg tttttaagca aacgagagca
    42961 ggatcacctg ctctcgcttg aaattattct cccttgtccc catctctccc tcatcctgtt
    43021 tttaacctta aaatggcatt attgaggtag acctacatga aaggacaaga aactcgtggt
    43081 tttcagtcag aagtgaaaca gcttctgcac ctgatgatcc attctctcta ttccaataaa
    43141 gaaatcttcc tgcgtgagct tatctctaac gcctccgatg cggcggacaa gttgcgtttc
    43201 cgtgcgctct ctaacccgga cctgtacgaa ggtgatggcg aactgcgcgt tcgtgtctct
    43261 ttcgataaag acaagcgcac gctgaccatc tccgataacg gcgtggggat gacccgcgac
    43321 gaagtgattg accatctggg gactatcgct aaatccggta ccaaatcatt cctcgaatcc
    43381 ctgggttctg accaggcgaa agacagccag ctgatcggtc agtttggtgt tggtttctac
    43441 tctgcgttta tcgtggccga caaagtgacc gtgcgtactc gcgcggcagg cgaaaaacca
    43501 gaaaacggcg tcttctggga atcggctggc gaaggtgaat ataccgttgc cgacatcacc
    43561 aaagaagatc gtggtactga aatcaccctg catctgcgtg aaggcgaaga cgagttcctc
    43621 gatgactggc gcgtgcgttc catcatcagc aaatactccg accatatcgc gctgccggta
    43681 gagatcgaaa aacgcgaaga gaaagacggc gaaaccgtta tctcctggga gaaaattaac
    43741 aaagcgcagg cgctgtggac tcgtaacaag tcggaaatca ccgatgaaga gtacaaagag
    43801 ttctacaaac acatcgccca cgattttaac gatccgctga cctggagcca caaccgtgtt
    43861 gaaggtaagc aggagtacac cagcctgctg tacattccgt cccaggctcc gtgggatatg
    43921 tggaaccgcg atcataaaca cggcctgaaa ctgtatgttc agcgtgtgtt catcatggac
    43981 gacgcagaac agttcatgcc gaactatctg cgcttcgtgc gtggtctgat tgactccagc
    44041 gatctgccgc tgaacgtttc ccgtgaaatc ctccaggaca gcacggtaac gcgtaacctg
    44101 cgcaatgcgc tgaccaagcg tgtgctgcaa atgctggaaa aactggcgaa agacgacgcg
    44161 gaaaaatacc agaccttctg gcaacagttt ggcctggtac tgaaagaagg tccggcggaa
    44221 gatttcgcta accaggaagc gatcgccaaa ttgctgcgtt ttgcttctac ccataccgat
    44281 tcttctgcgc agaccgtgtc tctggaagac tacgtttccc gcatgaaaga agggcaggag
    44341 aaaatctact acatcactgc agacagctat gcggcagcga agagcagccc gcacctggaa
    44401 ctgctgcgta agaaaggcat cgaagttctg ctgctttccg accgcatcga tgagtggatg
    44461 atgaactatc tgactgagtt cgacggtaaa ccgttccagt cggtgtctaa agttgacgag
    44521 tcgctggaaa aactggctga cgaagttgat gagagcgcga aagaagcgga gaaagcactg
    44581 actccgttca tcgaccgtgt gaaagccctg ctcggcgagc gcgtgaaaga tgtccgtctg
    44641 actcaccgtc tgaccgatac gccagcgatc gtttcgaccg acgcggacga aatgagcact
    44701 cagatggcga aactgttcgc tgcggcgggc cagaaagtgc cagaagtgaa atacatcttc
    44761 gaactgaacc cggatcacgt actggtgaaa cgtgcggcag atactgaaga tgaagcgaag
    44821 ttcagcgagt gggtagaact gctgctggat caggcactgc tggcagaacg cggcacgctg
    44881 gaagatccga acctgtttat tcgtcgtatg aaccagctgc tggtttcctg atgtaatgcc
    44941 ggatgacctt cgtgtcatcc ggcatttttc ttttcattat ctgcactttc cgcaaattat
    45001 ctcgccatta accgtttcag cccctggtgc ctttcttgag gcaatcgcct gatggtggta
    45061 tcgtttatcg ctttttcaaa aaattcgaca cattttaagg ggattttcgc aatgcgtatc
    45121 attctgcttg gcgctccggg cgcggggaaa gggactcagg ctcagttcat catggagaaa
    45181 tatggtattc cgcaaatctc cactggcgat atgctgcgtg ctgcggtcaa atctggctcc
    45241 gagctgggta aacaagcaaa agacattatg gatgctggca aactggtcac cgacgaactg
    45301 gtgatcgcgc tggttaaaga gcgcattgct caggaagact gccgtaatgg tttcctgttg
    45361 gacggcttcc cgcgtaccat tccgcaggca gacgcgatga aagaagcggg catcaatgtt
    45421 gattacgttc tggaattcga cgtaccggac gaactgatcg ttgaccgtat cgtcggtcgc
    45481 cgcgttcacg cgccgtctgg tcgtgtttat cacgttaaat tcaatccgcc gaaagtagaa
    45541 ggtaaagacg acgttaccgg tgaagaactg actacccgta aagacgatca ggaagaaacc
    45601 gtacgtaaac gtctggttga ataccatcag atgacagcac cgctgatcgg ctactactcc
    45661 aaagaagcgg aagcgggtaa caccaaatac gcgaaagttg acggcaccaa gccggttgct
    45721 gaagttcgcg ctgatctgga aaaaatcctc ggctaatcaa gccgcagcag atctgctttc
    45781 gggcaggtct gctcatccac ctcaggcaat acctctcaca gcaattagtt cttcttcctc
    45841 acttttccgc tacaattatc aacaagttga atcgataaga ggcggtaatg cgtcagacta
    45901 aaaccggtat cctgctggca aacctgggta cgcccgatgc ccccacacct gaagcggtaa
    45961 aacgctatct gaaacaattt ttaagcgaca gacgcgtggt tgatacctca cggttgttat
    46021 ggtggccatt gctgcgcggc gtgattttgc cgctgcgctc gccgcgtgtg gcgaagctgt
    46081 atgcctctgt ctggatggaa ggtggctcgc cgctgatggt ttacagccgc cagcaacagc
    46141 aggcgctggc acaacgttta ccggagacgc ccgtagcgct gggaatgagc tacggctcgc
    46201 catcactgga aagcgccgta gatgaactcc tggcagagca tgtagatcat attgtggtgc
    46261 tgccgcttta tccgcaattc tcctgttcta cggtcggtgc ggtatgggat gaactggcac
    46321 gcattctggc gcgcaaacgt agcattccgg ggatatcgtt tatacgtgat tacgccgata
    46381 accacgatta cattaatgca ctggcgaaca gcgtacgcgc ttcttttgcc aaacatggcg
    46441 aaccggatct gctgctgctc tcttatcatg gcattcccca gcgttatgca gatgaaggcg
    46501 atgattaccc gcaacgttgc cgcacaacga ctcgtgaact ggcttccgca ttagggatgg
    46561 caccggaaaa agtgatgatg acctttcagt cgcgctttgg tcgggaaccc tggctgatgc
    46621 cttataccga cgaaacgctg aaaatgctcg gagaaaaagg cgtaggtcat attcaggtga
    46681 tgtgcccggg ctttgctgcg gattgtctgg agacgctgga agagattgcc gagcaaaacc
    46741 gtgaggtctt cctcggtgcc ggcgggaaaa aatatgaata tattccggcg cttaatgcca
    46801 caccggaaca tatcgaaatg atggctaatc ttgttgccgc gtatcgctaa agctgagcgg
    46861 taaagaactg agcgccgtcg cgaagagcct cgtcggcggt tttcatcatc cgtgaataat
    46921 gcaaaaaggc gtgcagcgtg cctgggtaga gtttgaactc acagggctgc tgatgcgccg
    46981 ctaacgtctg gtaaagcaga cggctgtcat ccagcagcgg atcgaactcc gccccggcaa
    47041 taaaacaggg cggaacttcg cgagtgagat cattattaaa cagacagtaa tacggcgact
    47101 cgcggtccgc gtcgttgctt aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta
    47161 agccatccca gacaccgccc aacagacgac gagtcacgga atcccgtaat ccgtaaagcc
    47221 cataccacag caaaacgccc gcaactttac cgcaatcgat ctgtttatca cgcaaccaca
    47281 acgcactggc gagcgccagc atggcacctg cggaatcacc ggcaaagcca atgcgggaca
    47341 tattgatttg ataatcctcc gcctgctggt ggaaataaca acaagcagcc acaatttcct
    47401 ctatcacttg cggaaaacgc gcttcaggtg aaagggtgta atcaatacca atcaccgtac
    47461 attggctgta gcttgccagc aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa
    47521 taaaaccgcc tccatgcaaa taaaatagcg ttgccgggct atctggctgc ggacaaaaga
    47581 gacgtgtttc cacctgccca tattttgttg gaaccatgta agctttggtt gccatttctg
    47641 gagcgcccgc attccagaat cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg
    47701 ttcccgttgc gggccagggc ggtaaatccg gctgaagagt attcacaacg gtcttcattt
    47761 cagcagaaat aaggtccaga acaggtaatt tgttttccgg cttcatacaa aaactccttt
    47821 caaattacgt cattgtaagg aaccactgcc atgaaaatgc gatcccgcct gctgatattg
    47881 aaactggctg cgtctcgcgc gctcccgtca gattgtgtta acattcgccg ctcagttaac
    47941 cacccgtaaa aacaaccatg aaatttcccg gtaaacgtaa atccaaacat tacttccccg
    48001 taaatgcacg cgatccgctg cttcagcaat tccagccaga aaacgaaacc agcgccgcct
    48061 gggtagtggg tatcgatcaa acgctggtcg atattgaagc gaaagtggat gatgaattca
    48121 ttgagcgtta tggattaagc gccgggcatt cactggtgat tgaggatgat gtagccgaag
    48181 cgctttatca ggaactaaaa cagaaaaacc tgattaccca tcagtttgcg ggtggcacca
    48241 ttggtaacac catgcataac tactcggtgc tcgcggacga ccgttcggtg ctgctgggcg
    48301 tcatgtgcag caatattgaa attggcagct atgcctatcg ttacctgtgt aacacctcca
    48361 gccgtaccga tcttaactat ctacaaggcg tggatggccc gattggtcgt tgctttacgc
    48421 tgattggcga gtccggggaa cgtacctttg ctatcagccc tggccacatg aaccagctgc
    48481 gggctgaaag tattccggaa gatgtgattg ccggagcctc ggctctggtt ctcacctctt
    48541 atctggtgcg ttgcaagccg ggtgaaccca tgccggaagc aaccatgaaa gccattgagt
    48601 acgcgaagaa atataacgta ccggtggtgc tgacgctggg aactaagttt gtcattgccg
    48661 agaatccaca gtggtggcag caattcctca aagaccacgt ctctatcctt gcgatgaacg
    48721 aagatgaagc cgaagcgttg accggagaaa gcgatccgtt gttggcatct gacaaggcgc
    48781 tggactgggt agatctggtg ctgtgcaccg ccgggccaat cggcttgtat atggcgggct
    48841 ttaccgaaga cgaagcgaaa cgtaaaaccc agcatccgtt gctgccgggc gctatagccg
    48901 aattcaacca gtatgagttt agccgcgcca tgcgccacaa ggattgtcag aatccgctgc
    48961 gtgtctattc gcacattgcg ccgtacatgg gcgggccgga aaaaatcatg aacaccaacg
    49021 gagcagggga tggcgcactg gcagcgttgc tgcatgacat taccgccaac agctaccatc
    49081 gtagcaacgt accaaactcc agcaaacata aattcacctg gttaacttat tcatcgttag
    49141 cgcaggtgtg taaatatgct aaccgtgtga gctatcaggt actgaaccag cattcacctc
    49201 gtttaacgcg cggcttgccg gagcgtgaag acagcctgga agagtcttac tgggatcgtt
    49261 aagttatcgt cggttcgtag gccagataag gcgttcacgc cgcatctggc attttgctct
    49321 caatgcctga tgcgacgctg gcgcgtctta tcatgcctac atatttttca tattttacat
    49381 ccggcaacca ccgtttaccc cgtcaccact tcacccgccg gtggcgtttc cagcagttcc
    49441 agcatagtac gggcaatttc acgctcgccc atcactactt gattcgcacc acgttcggtg
    49501 atatacgcca cttcatcgtc ataatgggcg cgggcaataa tctcaatatc cggatttttc
    49561 gcgcgggcag atgccacaat ctcacccgct tcataaccgt tggggatcgt caggatcagc
    49621 cattttgcac attccagatg cgccagttgc ataatttctt cgttcgccgc attgcccaat
    49681 actgcgcgga ccccgcgctc tcgcagctca tcaacacggg ttcgtgacgt ctcaatcacc
    49741 accagcggaa tatcagaggc gagcaatttc tcccccagca ggctgcctac acgaccgtaa
    49801 cccaccaata gcgcatggtt gcaaatatcc actgggatct gcttctcttc ttcgattgcc
    49861 tcttccagcg tctgctcttc cagcgtttcg gtcttcgcca gatatttctc cagtagtgcg
    49921 aacagtactg ggttgagcat aatcgacagg atcgcccctg ccagtaccag gttttgtccg
    49981 gcctgcggca gtaaattcaa tgccattccc agtcccgcca ggataaacgc gaactcacca
    50041 atctgcgcca ggctggcggc gatagttaat gccgtacgtt gggagtgacc aaacagtcgc
    50101 accaggaaaa acgcggctaa cgacttacca aacagaataa tcgccagcgt cgccagcact
    50161 gccagcggtt gctgaatcag aattaacgga tcaaacaaca tcccgacgga gacaaaaaac
    50221 agcaccgcaa acgcgtcgcg caatggcagc gtatcgtggg cggcacggtg actcagttca
    50281 gactcgttca gtaccatccc ggcaaagaac gcaccgagtg caaaggagac atcaaacagc
    50341 tctaccgcac caaaggcaat ccctaacgcc agcgccagca ccgacagggt aaacagctcg
    50401 cgagaaccgg ttgccgcgct gcgtgccata atccacggca ccagacggcg acctaccagc
    50461 atcataatgg cgataaacgc gatcactttg ccgatggtga tccccatatc gactgcaaga
    50521 gtggcaaagc ccacatcgcc ctgttccatc attcctgcca ctgcgggcag caacaccagc
    50581 gtcagaacca ttaccaggtc ttccacaatc aaccaaccga tggcgatttg cccacgctga
    50641 ctgtcaatta attgccgttc ttcaagtgcg cgcagtaaca ccacggtact ggcggtggaa
    50701 agacataaac cgaacacgat accggtcatt aacgaccagc ccagcacggc agagagcgcc
    50761 atacccagca gcgtcgccac ggctatctgg gcgatcgcac cgggaatggc gatggccttt
    50821 accgccatca aatccttgag cgaaaagtgc agacccacgc caaacatcaa cagaatgacg
    50881 cccagttcag ccagttccgg cgcaagcttg gtatcggcaa caaagcccgg agtgaatggt
    50941 cctgccagca cacccgctaa cagatatccc accagaggag aaatacgtag tttattggcc
    51001 agcatgccga ggataaaggc gagcacaagg ccgccaacaa tggtggtgat aagcggggtg
    51061 gcgtgatgca ttccgtctcc ttttcctggt ggttattgtc catttttggc cgggaaaacc
    51121 aaaattacag gtaatagttt atgacaattt cattgatgat gttcatgaat aattattgaa
    51181 ttttgcagaa aaatggaatt agctgcaaaa aagcacggat cggaaaacag aaggcggttt
    51241 aacagaggaa aggcttatgg cgactgtaaa acgatgcagc caaagtttgc ctttggctgc
    51301 aatgaaatca gtctttatgc cggttatcag gcaggaatat ggtcaacatc cccaatagtg
    51361 gcaggaaagc acatatttta tagactaact cgatgctggt gtgatcggcg ataagcccca
    51421 gaactgccgc tcccagacct cccatgccga aagcaaaacc gaaaaagagt ccagaaacca
    51481 taccgatacg tcctggaagc agctcctgag cgtagaccag aatggcagag aatgccgaag
    51541 cgaggataaa tccaataatc accgttaaaa cccccgtcca gtgcaggctg gcgtagggta
    51601 aaatcagcgt aaacggcgca acgccgagga tagagcccca aatcacatat ttccgcccaa
    51661 ttttatcccc tacaggcccg ccgatcaccg tacctgccgc aacggcaaac aggaaggcaa
    51721 acagatgaag ctgagcattc tggatagata atccgaattt ttgcatcaga taaaaggtgt
    51781 aatagctgct gatgctcgcc atatagaaat atttcgagaa aatgaggatt aacagaatgc
    51841 tgaccgccag taccactttg ttgcgcggca gtggattgat aatcgtcgct ttgggttttc
    51901 ctttattcat tcggtgctgt gccgagtacc aacggctgat ttgcgccaac accacgatcg
    51961 ccagcagtgc cgcaagcaca aaccaggcaa cgttgccttt gccataaggc gcgataatca
    52021 ccgccgccag caagggtccc agggaactgc caaagttgcc gccgacctga aagatagatt
    52081 gtgccaggcc atgccgcccg ccggaagcca tacgggccac gcgagaagat tccggatgaa
    52141 agaccgatga accggtaccg accagcgccg ccgccagcag aactgcgcca aaactgcccg
    52201 ccagcgcaag cagcaccaga ccacttaagg taaagcacat gccaattggc aacgaccacg
    52261 gcatcggata tttatcggtc cagtagccga ccactggttg cagtagcgaa gaggcgagct
    52321 ggaaggtgag ggttatcatg ccaatctgca taaatgtcag agaaaattct gactgaagca
    52381 gaggataaat cgccagaatc agcgattgga tcatgtcgtt cagcagatgt gagaggctga
    52441 tagcacctaa aataccaaac gatgttcggg ccttggtcgt tgacgcagcc gcgcccgcca
    52501 caggctgggt ttgttcactc attgccatag gaaagtcact ttttcagggt tgcgatgtta
    52561 agaatgatct tatttgtgat tattacccga ctaacatacc tgtatgcgcc gcctgaagga
    52621 agtctcaacg ctgaatacag aatttctaat ctggatgcag atttatcttc accggacgca
    52681 gacttgtcta tgatgtcgcg tcatactatt tttcaacacg ttgaaatcag gtcagggaga
    52741 gaagtatgaa attattgcag cggggcgtgg cgttagcact gttaaccaca tttacactgg
    52801 cgagtgaagc tgctctggcg tatgagcagg ataaaaccta caaaattaca gttctgcata
    52861 ccaatgatca tcatgggcat ttttggcgca atgaatatgg cgaatatggt ctggcggcgc
    52921 aaaaaacgct ggtggatggt atccgcaaag aggttgcggc tgaaggcggt agcgtgctgc
    52981 tactttccgg tggcgacatt aacactggcg tgcccgagtc tgacttacag gatgccgaac
    53041 ctgattttcg cggtatgaat ctggtgggct atgacgcgat ggcgatcggt aatcatgaat
    53101 ttgataaccc gctcaccgta ttacgccagc aggaaaagtg ggcaaagttc ccgttacttt
    53161 ctgccaatat ctaccagaaa agtaccggcg agcgcctgtt taaaccatgg gcgctgttta
    53221 agcgtcagga tctgaaaatt gccgttattg gtctgacgac ggatgacaca gcgaagttag
    53281 gcaatccaga aaacttcacc gatattgagt tccgtaagcc cgccgatgaa gcgaagctgg
    53341 tgattcagga gctgcaacag acagaaaagc cagacattat tatcgcggcg acacatatgg
    53401 ggcattacga taacggcgat cacggttcta acgcaccggg cgatgtggag atggcacgcg
    53461 cgttgcctgc cggatcgctg gcgatgattg tcggcggtca ctcgcaagat ccagtctgta
    53521 tggcagcgga aaataaaaag caggtagatt acgttccagg tacgccatgc aaaccggatc
    53581 aacaaaacgg catctggatt gtacaggcgc atgagtgggg caagtacgtt ggtcgggccg
    53641 actttgagtt tcgcaacggc gaaatgaaaa tggttaacta ccagctgatt ccggtgaacc
    53701 tgaagaagaa agtgacctgg gaagacggga aaagcgagcg cgtgctgtac acccctgaaa
    53761 tcgctgaaaa ccagcaaatg atctcgctgt tatcgccgtt ccagaacaaa ggcaaagcgc
    53821 agctggaagt gaaaataggc gaaaccaatg gtcgtctgga aggcgatcgt gacaaagtgc
    53881 gttttgtaca gaccaatatg gggcggttga ttctggcagc acaaatggat cgcactggtg
    53941 ccgactttgc ggtgatgagc ggaggcggaa ttcgtgattc tatcgaagca ggcgatatca
    54001 gctataaaaa cgtgctgaaa gtgcagccat tcggcaatgt ggtggtgtat gccgacatga
    54061 ccggtaaaga ggtgattgat tacctgaccg ccgtcgcgca gatgaagcca gattcaggtg
    54121 cctacccgca atttgccaac gttagctttg tggcgaaaga cggcaaactg aacgacctta
    54181 aaatcaaagg cgaaccggtc gatccagcga aaacttaccg tatggcgaca ttaaacttca
    54241 atgccaccgg cggtgatgga tatccgcgcc ttgataacaa accgggctat gtgaataccg
    54301 gctttattga tgccgaagtg ctgaaagcgt atatccagaa aagctcgccg ctggatgtga
    54361 gtgtttatga accgaaaggt gaggtgagct ggcagtaatc cgaaagtgcc ggatgttcgc
    54421 atccggcaca atgcttaatc gcggcgggcg atatcagcaa atttggcatc gaggatcttc
    54481 gccagatcgc ctgccgccag ttcgatatcc agtccgcgct tgccgccgga aacataaata
    54541 gtggcaaatt cttgtgcggg ggcgtcgata atcgttggca gacgtttttt ctgccccagt
    54601 gggctaatcc ccccaaccag gtatcccgtc gaacgctgcg cgaccatcgg atcggccatc
    54661 tcaactttct tggcacccag cgcttttgct acttttttaa ggtccagttg acctgcgacc
    54721 ggcgtaacgg ccacggcaag gtgtttcata tcaccgttca ctgcgaccag cagcgttttg
    54781 tagacctgat ccggattcaa acctaatttt ttgacgactt catcgccaaa attggtttca
    54841 gccggatcgt gctcgtaggt atggatttga aacgaaatct tgtttttttc gagtgattta
    54901 actgcgggtg tcatggtaat tttcctgcct taaacaaaaa aacgaagcaa gcatacgcct
    54961 gatggcggtc caaaaaaaag agtcatcttg cctaagagta ttggcaggat ggtgagattg
    55021 agcgacaatc gagttacacc gtcgcttaaa gtgacggcat aataataaaa aaatgaaatt
    55081 cctctttgac gggccaatag cgatattggc cattttttta gcgcaacatt tgcggtaaat
    55141 tcccttctcc atacaggtgt agtgcaccga ccgcgaccac atatcgcccc ggcggcatgg
    55201 cgcgtaattt atcccgccag gcgagatttc gctgatgcat cagcacatcg tacagcgact
    55261 gactgaacgt attgggcagc gttatttcat tattttgcgg cggcgcattc agccaccagc
    55321 tcatcatttg ttgcagcaac cgtgcgttgg tatgccagtg ggtcagcgta tcgtccagca
    55381 gcgccagtcc tttgtcaggg agctggagca acatggcaat ctggttttcc gccccttcca
    55441 gttcaatcac gggtttatgt tgttgcttcg ccgcctgcaa tagctgataa tcgataccgt
    55501 attctgcccg cagccccagt ttttgtgcct gcgtcgcctg aagaaccatc gcgatttgcc
    55561 acagcggttg ggtagaaaag agtgagggag aaatgcccat ctcctggcta atgtgctgca
    55621 gtttttggag ttgctcctcg ctaatgcgct cttccagcgc ctcgcaggca ggcaaattag
    55681 caaaaggcgt atcgctggtg gaaacatccg cctcgacgat cagcgcatcg gcgtttttga
    55741 gctttttgag caaacgggtg ggtaggggag ccatatcgtg gctacccata tgaatactgc
    55801 caatcagatg aaaatggcga ttgccgggaa gagagatatc aatggcaggc caggtgtaat
    55861 gattaccgcg cagcgcggcc caaagcgttt ttacccggta caacagatcc atacgacctc
    55921 cctttgtgaa atatcatgct agcgcgcggg gagggatggc gcaaatggag ttggggactt
    55981 gtttaatgtg tttgtatgat tcagtatgtt cttgtatcgc tatttacagg gaagcaacag
    56041 ttaaaaacta tgaaacaggc aacaagaaaa ccgacgacac ccggagatat tcttctctat
    56101 gaatatctgg aaccgctaga tttgaaaatc aatgagttag cagagttgct gcatgttcat
    56161 cgtaatagcg tcagtgcact gatcaataac aatcgtaaac tcaccactga gatggcattc
    56221 cgtctggcga aagtttttga taccacagtc gatttttggc taaacctcca ggcggcagtt
    56281 gatctttggg aagttgaaaa caacatgcgc acccaggaag aattgggacg gattgaaaca
    56341 gtggctgaat atttggcacg ccgtgaagag cgtgcaaaaa aggtcgcgta aggccaaaac
    56401 atcgcttcat ctcgtaggcc ggataaggcg ttcacgccgc atccggcatc cgagcaccat
    56461 tgcctgatgc gacgcttacg cgtcttatca ggcctacaaa cctgtgcctg aaccgtaggc
    56521 cggataaggc gtttacgccg catccggcag tcatgcgtcg atgccaaatg cgccacccta
    56581 aagcagcgca tccgcaatga cgtacttatt ccttcggttt aaaccgcagc aaccggttgg
    56641 cgttactcac tacggtaatc gacgagagcg ccattgccgc tccggcaact accgggttaa
    56701 gcagtgttcc agtaaacggc cacaaaatac cggcggcgac cggaataccg atactgttgt
    56761 agataaacgc gccgagcagg ttctgcttca tgttgcgcag cgttgcgcgg gaaatagcga
    56821 gtgcatccgc aacgcccatc aggctatggc gcatcagggt aatcgccgcg gtttcaatgg
    56881 caacatcact gccgccgccc attgcaatgc cgacatccgc ctgggccagc gctggcgcat
    56941 cgttaatgcc gtcgcccacc attgccacct gacgtccttc actttgcagg cgtttaatcg
    57001 cttcggcttt accgtccggc agcaccccgg cgatcacctc atcaatccct gcttctttgg
    57061 cgatcgcatt ggcggtggtt gggttatccc cggtcaacat caccagacga tatcccgctt
    57121 tatgcagacg ttgcagcgcc gccacgctat cactacgcaa cggatcgcgt acagccagca
    57181 gggctaccgc tttcccgtca accgccagca gcacaggcgt tgccccttgc gatgcctgag
    57241 ctgaaatatc tgcttcaatc gctttagtat caacttgctg gtcatttaac agcgcctgat
    57301 tgcccagcaa taacgcatga ccttcagctt caccgctcac gcccagcccg cgcaatgtgc
    57361 ggaaaccgtt gacctgcggc agctgcatat cgcttgcttt atcgaggatc gcccgcgcca
    57421 gcggatggct ggaaccttgt tccagtgccg ccgccagacg cagcgcctgc gcttcgtcaa
    57481 aatcagcaaa tgttttcact gcgacaacct gcggcttccc ttcggtcagc gtcccggttt
    57541 tatcgaacac tacggtatcg agtgtactag cgcgttgcag cgcgtcggcg tcccgcacca
    57601 gcacgccaaa ctcagccgcc cgcccgacgc cggaaataat cgacatcggc gtcgccagcc
    57661 ccagcgcaca cggacaggca ataatcagca ccgtggtggc aatcaccagg gtatagacaa
    57721 tctgcggtgc cggaccaaag aaataccaga ttgccgcact gacaagcgca ataaccacta
    57781 ctaccggcac aaatacggct gagattttat ccgccagctg accgatttct ggcttgctgc
    57841 tctgggcctg gcgcaccata cgaataatcc ttgatagcgt ggtatggctg ccaaccgcac
    57901 tggcgcgaaa cagaacgcta ccgtcctgta ccactgtccc ggcatggacg ctatcgcctt
    57961 cgcctttttg ctgcgggata ggttcgcccg tcagcatcgc ttcatccagc catgcttcgc
    58021 cctgggtaat ctcgccatct actggcacgc ggtcgcccgt cgtcaggcgc agtagcatgc
    58081 ctggctgaac ttccgccagc ggcacgcttt tttcaccttc gtcagtaacc aggcgcgccg
    58141 tcggcggagt taaatcgagt aatttctcca gcgcctttga agaacgctga cgtgcgcgcg
    58201 cttccagcat atgaccgaga ttaatcagac cgataatcat cgcactggct tcatagtaaa
    58261 gatgccgcgc ttccatcggg aaccactgcg gccacaggtt gacgctcatc gaatagagcc
    58321 acgccacacc cgtacccagc gccaccagcg tatccatcgt cgccgcaccg ttcagcaggc
    58381 ttttccatgc actgcggtaa aaatggccgc cggcgaaaac catcactgcc agggttatca
    58441 ggccgataac caaccacagg ctgcggttgt cagcggtgac catcatgtta tcgccgatca
    58501 tcccccagac cattaccggg atacccacag ccagtgcaac aattgcctgc cagcggaagc
    58561 gcttcatcgt agcgacggcg gtttcttgct ggcgctcgcg gcgtttcgcg tcatcttcaa
    58621 tcgcttccgc gccgtagccc gctttttcca ccgcctgcac taaatcttgt ggggaggcac
    58681 tgcccatcac cagcgcagta cgctccgcca ggtttacccg tgcctgagtg acgcccggta
    58741 cgctttgcag cgcattttgt acgcgggtga cacagctggc gcagctcatg ccgctcagca
    58801 gcaactgctg gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg
    58861 tcagtgcttc cgacgggatt gatgactccg ccagcggttt agcctttggg tggcttacag
    58921 atgcgtcata acccgcttgt ttgatggttt cgatcagctg ttctgcactg gcagtcccgg
    58981 taacgtgcgc ttcagtgata gacacatccg cttgttccac atccgggcgc tgttcgagac
    59041 tttctttcac gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc aggtcgatag
    59101 tttgtgacat aaaacactcc tttaagacag ttttgactgg ctgtgataaa ggttaaacct
    59161 tccagcaagg ggaaggtcaa gaaattaata acccaggcga gtaaaagtcc gtaaagatta
    59221 aaaaatcggc tcgatttgca tcaggattag acatttatct ctttgttttc ctgtagttaa
    59281 gttgcgggtg ctaagttaaa tcaggatgcc tgaaaatcgg caccggggtg aggaattacc
    59341 tcccgcatct ataaaaagga gttaacaaaa gatgttagat gcaaacaaat tacagcaggc
    59401 agtggatcag gcttacaccc aatttcactc acttaacggc ggacaaaatg ccgattacat
    59461 tccctttctg gcgaatgtgc ctggtcaact ggcagcagtg gctatcgtga ccagcgatgg
    59521 caacgtctat agtgcgggtg acagtgatta ccgctttgcg ctggaatcca tctcgaaagt
    59581 ctgtacgtta gcccttgcgt tagaagatgt cggcccgcag gcggtacagg acaaaattgg
    59641 cgctgacccg accggattgc cctttaactc agttatcgcc ttagagttgc atggcggcaa
    59701 accgctgtcg ccactggtaa atgctggcgc tattgccacc accagcctga ttaacgctga
    59761 aaatgccgaa caacgctggc agcgaatttt acatatccaa cagcaactgg ctggtgagct
    59821 ggtagcgctc tctgacgaag tcaaccagtc ggaacaaaca accaacttcc ataaccgggc
    59881 cattgcctgg ctgctgtact ccgccggata tctctattgt gatgcaatgg aagcctgtga
    59941 cgtgtacacc cgtcagtgct ccacgctcat caatactgtt gaactggcaa cgcttggcgc
    60001 gacgctggcg gcgggtggtg tgaatccgtt gacgcataaa cgcgttcttc aggccgacaa
    60061 cgtgccgtac attctggccg aaatgatgat ggaagggctg tatggtcgct ccggtgactg
    60121 ggcgtatcgt gttggtttac cgggcaaaag cggtgtaggt ggcggtattc tggcggtcgt
    60181 ccctggagtg atgggaattg ccgcgttctc accaccgctg gacgaagaag gcaacagtgt
    60241 tcgcggtcaa aaaatggtgg catcggtcgc taagcaactc ggctataacg tgtttaaggg
    60301 ctgatcatga tgaacacgga aggtaataac ggtaacaaac ccctcggtct atggaacgtc
    60361 gtttccatcg gcattgggg
//
LOCUS       DAZFDC010000013        54305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFDC010000013 DAZFDC010000000
VERSION     DAZFDC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.013"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2294)
                     /gene="pbpC"
                     /locus_tag="ACHJ48_000958"
     CDS             complement(<1..2294)
                     /gene="pbpC"
                     /locus_tag="ACHJ48_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO4706567.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEVA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEALPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASV"
     gene            complement(2295..7256)
                     /gene="yfhM"
                     /locus_tag="ACHJ48_000959"
     CDS             complement(2295..7256)
                     /gene="yfhM"
                     /locus_tag="ACHJ48_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO4706568.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADEFGSNESKVIVAAPVIAELNMPRF
                     MASGDNSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRDEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            7463..8308
                     /gene="sseA"
                     /locus_tag="ACHJ48_000960"
     CDS             7463..8308
                     /gene="sseA"
                     /locus_tag="ACHJ48_000960"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO4706569.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            8651..8776
                     /gene="timP"
                     /locus_tag="ACHJ48_000961"
     CDS             8651..8776
                     /gene="timP"
                     /locus_tag="ACHJ48_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO4706570.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVINNLQSSR"
     gene            complement(8808..9584)
                     /gene="sseB"
                     /locus_tag="ACHJ48_000962"
     CDS             complement(8808..9584)
                     /gene="sseB"
                     /locus_tag="ACHJ48_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO4706571.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(9727..11010)
                     /gene="pepB"
                     /locus_tag="ACHJ48_000963"
     CDS             complement(9727..11010)
                     /gene="pepB"
                     /locus_tag="ACHJ48_000963"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO4706572.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVASDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(11188..11388)
                     /gene="iscX"
                     /locus_tag="ACHJ48_000964"
     CDS             complement(11188..11388)
                     /gene="iscX"
                     /locus_tag="ACHJ48_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO4706573.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(11400..11735)
                     /gene="fdx"
                     /locus_tag="ACHJ48_000965"
     CDS             complement(11400..11735)
                     /gene="fdx"
                     /locus_tag="ACHJ48_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO4706574.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(11737..13587)
                     /gene="hscA"
                     /locus_tag="ACHJ48_000966"
     CDS             complement(11737..13587)
                     /gene="hscA"
                     /locus_tag="ACHJ48_000966"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO4706575.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(13604..14119)
                     /gene="hscB"
                     /locus_tag="ACHJ48_000967"
     CDS             complement(13604..14119)
                     /gene="hscB"
                     /locus_tag="ACHJ48_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO4706576.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(14215..14538)
                     /gene="iscA"
                     /locus_tag="ACHJ48_000968"
     CDS             complement(14215..14538)
                     /gene="iscA"
                     /locus_tag="ACHJ48_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO4706577.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(14555..14941)
                     /gene="iscU"
                     /locus_tag="ACHJ48_000969"
     CDS             complement(14555..14941)
                     /gene="iscU"
                     /locus_tag="ACHJ48_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO4706578.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(14969..16183)
                     /gene="iscS"
                     /locus_tag="ACHJ48_000970"
     CDS             complement(14969..16183)
                     /gene="iscS"
                     /locus_tag="ACHJ48_000970"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO4706579.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(16295..16783)
                     /gene="iscR"
                     /locus_tag="ACHJ48_000971"
     CDS             complement(16295..16783)
                     /gene="iscR"
                     /locus_tag="ACHJ48_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO4706580.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(17084..17821)
                     /gene="trmJ"
                     /locus_tag="ACHJ48_000972"
     CDS             complement(17084..17821)
                     /gene="trmJ"
                     /locus_tag="ACHJ48_000972"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO4706581.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            17940..18743
                     /gene="suhB"
                     /locus_tag="ACHJ48_000973"
     CDS             17940..18743
                     /gene="suhB"
                     /locus_tag="ACHJ48_000973"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO4706582.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            18888..19742
                     /locus_tag="ACHJ48_000974"
     CDS             18888..19742
                     /locus_tag="ACHJ48_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4706583.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVNFI
                     LSALNPQN"
     gene            19933..21213
                     /gene="csiE"
                     /locus_tag="ACHJ48_000975"
     CDS             19933..21213
                     /gene="csiE"
                     /locus_tag="ACHJ48_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO4706584.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(21205..22344)
                     /locus_tag="ACHJ48_000976"
     CDS             complement(21205..22344)
                     /locus_tag="ACHJ48_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO4706585.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(22504..23394)
                     /gene="hcaR"
                     /locus_tag="ACHJ48_000977"
     CDS             complement(22504..23394)
                     /gene="hcaR"
                     /locus_tag="ACHJ48_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO4706586.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            23530..24891
                     /gene="hcaE"
                     /locus_tag="ACHJ48_000978"
     CDS             23530..24891
                     /gene="hcaE"
                     /locus_tag="ACHJ48_000978"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO4706587.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            24888..25406
                     /gene="hcaF"
                     /locus_tag="ACHJ48_000979"
     CDS             24888..25406
                     /gene="hcaF"
                     /locus_tag="ACHJ48_000979"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO4706588.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEICYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            25406..25726
                     /locus_tag="ACHJ48_000980"
     CDS             25406..25726
                     /locus_tag="ACHJ48_000980"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO4706589.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            25723..26535
                     /gene="hcaB"
                     /locus_tag="ACHJ48_000981"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             25723..26535
                     /gene="hcaB"
                     /locus_tag="ACHJ48_000981"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO4706590.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            26545..27747
                     /gene="hcaD"
                     /locus_tag="ACHJ48_000982"
     CDS             26545..27747
                     /gene="hcaD"
                     /locus_tag="ACHJ48_000982"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO4706591.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            27844..28266
                     /locus_tag="ACHJ48_000983"
     CDS             27844..28266
                     /locus_tag="ACHJ48_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4706592.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(28314..29186)
                     /locus_tag="ACHJ48_000984"
     CDS             complement(28314..29186)
                     /locus_tag="ACHJ48_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO4706593.1"
                     /translation="MTIYTLSHGSLKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(29198..30292)
                     /locus_tag="ACHJ48_000985"
                     /pseudo
     CDS             complement(29198..30292)
                     /locus_tag="ACHJ48_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(30325..31323)
                     /locus_tag="ACHJ48_000986"
     CDS             complement(30325..31323)
                     /locus_tag="ACHJ48_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4706594.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSHHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(31348..32859)
                     /locus_tag="ACHJ48_000987"
     CDS             complement(31348..32859)
                     /locus_tag="ACHJ48_000987"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4706595.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIRAVKKMSALGVAVIYVSHRMEEIRRIASS
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAVGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(32882..33865)
                     /locus_tag="ACHJ48_000988"
     CDS             complement(32882..33865)
                     /locus_tag="ACHJ48_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO4706596.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(33962..37243)
                     /locus_tag="ACHJ48_000989"
     CDS             complement(33962..37243)
                     /locus_tag="ACHJ48_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO4706597.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTRLDAYEE
                     KRFEQFFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCD
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPINQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWCLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNEAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGTTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLHLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            37361..38554
                     /locus_tag="ACHJ48_000990"
     CDS             37361..38554
                     /locus_tag="ACHJ48_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO4706598.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(38617..39870)
                     /gene="glyA"
                     /locus_tag="ACHJ48_000991"
     CDS             complement(38617..39870)
                     /gene="glyA"
                     /locus_tag="ACHJ48_000991"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO4706599.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEVEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            40198..41388
                     /gene="hmpA"
                     /locus_tag="ACHJ48_000992"
     CDS             40198..41388
                     /gene="hmpA"
                     /locus_tag="ACHJ48_000992"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO4706600.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(41433..41771)
                     /gene="glnB"
                     /locus_tag="ACHJ48_000993"
     CDS             complement(41433..41771)
                     /gene="glnB"
                     /locus_tag="ACHJ48_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO4706601.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(41832..43166)
                     /gene="glrR"
                     /locus_tag="ACHJ48_000994"
     CDS             complement(41832..43166)
                     /gene="glrR"
                     /locus_tag="ACHJ48_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO4706602.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(43156..43869)
                     /gene="qseG"
                     /locus_tag="ACHJ48_000995"
     CDS             complement(43156..43869)
                     /gene="qseG"
                     /locus_tag="ACHJ48_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO4706603.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(44034..45461)
                     /gene="qseE"
                     /locus_tag="ACHJ48_000996"
     CDS             complement(44034..45461)
                     /gene="qseE"
                     /locus_tag="ACHJ48_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO4706604.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(46037..49924)
                     /gene="purL"
                     /locus_tag="ACHJ48_000997"
                     /gene_synonym="purI"
     CDS             complement(46037..49924)
                     /gene="purL"
                     /locus_tag="ACHJ48_000997"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO4706605.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            50181..51737
                     /gene="mltF"
                     /locus_tag="ACHJ48_000998"
     CDS             50181..51737
                     /gene="mltF"
                     /locus_tag="ACHJ48_000998"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO4706606.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMQ
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(51734..52237)
                     /gene="tadA"
                     /locus_tag="ACHJ48_000999"
     CDS             complement(51734..52237)
                     /gene="tadA"
                     /locus_tag="ACHJ48_000999"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO4706607.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(52295..52930)
                     /gene="yfhb"
                     /locus_tag="ACHJ48_001000"
     CDS             complement(52295..52930)
                     /gene="yfhb"
                     /locus_tag="ACHJ48_001000"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO4706608.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            53139..53987
                     /locus_tag="ACHJ48_001001"
     CDS             53139..53987
                     /locus_tag="ACHJ48_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO4706609.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            54043..54303
                     /gene="yfhL"
                     /locus_tag="ACHJ48_001002"
     CDS             54043..54303
                     /gene="yfhL"
                     /locus_tag="ACHJ48_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO4706610.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        12800 a        15029 c        13746 g        12730 t
ORIGIN      
        1 actgacgcga tttgccctac gtcatccatc accagcaatt gataatcacc tttatccgtc
       61 aaatgcaggg tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag
      121 cgttcacctg cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa
      181 cgtttaataa tctcgccatc gcgaacgcct gtcagctgca gcgggagctg ggcatcgtga
      241 ccgtatggcg ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg
      301 ggcaaccagg gttccagcgg tagcggccag acatttatca tttcttgtcg cgcttgcggg
      361 caatcagcgg caacacgttt gccattttca tccagccaga tggggaaacg aatgccatta
      421 atgccttcct gctccggcag taatagagtt ggcggctgac tcccgtccag cagccaggtt
      481 gccaggcggc ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcaa
      541 ataacgccac gagtgactga gttcgggcgc gggtcttccg gcagattagc actgcgcgac
      601 agtaagatat tattgacctg attcaacaat ggtacggcgc tggcaaaacc aaactgacca
      661 acaacgggcg tgccgtccgg tctgccagtc cagatcccaa tgacatagcg agcgttaacc
      721 ccaatcgccc aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg
      781 acgcgcggca aggcaccatc cggcaagggt agcgcttcat cagccataat ccggcgaatg
      841 atccacgccg ctcccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag
      901 cgcaatttgc ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc
      961 agtttcgcac cagcgccgcc gagaatgagc gaaagattcg gcgcagcacc gttgggcaaa
     1021 tataacggca atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc
     1081 agcacctgca cagcaggtaa attcagcgag cgcaccagcg cctcgctcat gctgatcggg
     1141 ccatgaaaac cgctgtcaaa gttgccaggt cggtaatcac cggtacgccg ggggacgtct
     1201 tgcagcagtg atgccgggtg gatcaatcct tcatccagcg ccagaccgta aacaaacggt
     1261 ttgagcaccg atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa
     1321 tcatcgttga gatcaaccga acccacccag ccgcgaacac gcatatcggt atgatcaacc
     1381 acgatcatcg ccagtgagct gcgcggtggt aatcgtcctt tccagttttg cgccagttct
     1441 tccagacgtc gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta
     1501 ccgagcatca tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag
     1561 atgggttctt cccttgactc ttttacctgc tcacgggacc acacaccttg cacggccatc
     1621 cgttcgagca ctttattacg cgcgacttcg gcacgctccg gccaacgatc cgggcgaaga
     1681 cggctgggcg cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca
     1741 ggcgactttc cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac
     1801 ggagcgcggt taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc
     1861 aacgcgcgcc agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga
     1921 cgagcaacct gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct
     1981 tgccatgctg cgcgcgccac cgagaatgga ttcaccccag gatgcttcca gaaccagcga
     2041 tcttcataat tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttacagga
     2101 taacgccaga tgccgtcagc atcggcgaag cgccagagcg gcgtaccatc ctgcgccacg
     2161 accactcgtg cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac
     2221 gctgcaagaa aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt
     2281 aacaagcgag gcatttacgg tctgacaatc agcaaatctt cagccgcgcc ggtcgcccgc
     2341 cattggggaa catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc
     2401 accgcccgcg ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc
     2461 acaaagcgat cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt
     2521 aagttctgca cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctgg
     2581 ttttccagtt ccagacccgc aggcagcaga tccacgacta acgcatccgg cacgctgtta
     2641 ctagctttta cctgcaacca gaccagcacc agatcgccgc tacgtaacga gtccagcgat
     2701 ttgctcttac catcagtacc aagaatatga cgctcaattt gcagcacatt gttcgccggt
     2761 aaaggtgcgg attgcggata accgctggca tccacacgca gccataacgg ctgctcgcca
     2821 ctgttggtca cctgcaaggt ggcaagttga tcgctattca gattgctgtt ttgcgctttc
     2881 tcgcctgtca gcggctcagc tgagaaagag gtttgcgcct gccatttacc tggtaaatcc
     2941 tgaatcgtac gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca
     3001 ccaaacgcct gctgcgaaag tgtgttcagc agggagtact gctcatcggg tagcagtttg
     3061 ttctcttcca gcaaggagag catcaacgcg ttgtcgcgca gtggactacc gtaatcaccc
     3121 agccatatcc gctcatcact attacgcggc gtttttagcg ccagcgcaat cgcttcttca
     3181 tcacgcgtcg catcacccat ggttttcagc gcaacgccaa gttgcagcag cggtaaacca
     3241 gaagcggcat ctgcacgatg ctcccagatt tcacgcagcg cacccagcgg agccttttgc
     3301 tgacgggcca acaccagcgc agcgtaagac tgtacggcga atttactggc tttgagatta
     3361 tccgcgtacg ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta
     3421 ccccggttaa tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa
     3481 tccatcacgt aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag
     3541 ccgccgttat cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg
     3601 cgtttctcat cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa
     3661 agtgacggga acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac
     3721 tctttgatat aacgtgcgat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc
     3781 agcgtaacag gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc
     3841 tgtaacgccg taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc
     3901 cactgcttat gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc
     3961 tgaatttctc catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc
     4021 acacctggcg ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg
     4081 gcggtcaggg caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc
     4141 agacgcgagt tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt
     4201 gcggcaacta tcactttgct ttcgttgcta ccgaattcat ctgccgtcca ggcttgcgcc
     4261 atgacgcgca attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt
     4321 tcgttgagcg ttaccggcag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt
     4381 ttaccaccac gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc
     4441 tgaccttcaa taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca
     4501 aagaacgcct gccacggatc tggcgtgacg tagtcagtaa tattcagcac accactatca
     4561 acggcagaca ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta
     4621 attttcacgg ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg
     4681 aggcgacggt tttcatcacc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc
     4741 gcggagcgag atttatcgcc aggacgcacc accagcgtac tcaaataaag atcatgacga
     4801 ttccaggttt tatcgaccgg aatcgtcaga tccagccctt gagccggaac atcaatctct
     4861 tgccaccaca gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc
     4921 gcggcgatat gcaacttaat ggtatcgcca gggcgataac cggctttatc cagtttcagc
     4981 gtgacacggt cgggtcgcac tgcgccgcta ccgtcgctat tatcctgcca gctatagcca
     5041 gcccaaaaac gaacactact gaccgcttca ttcggcgctt tgacttccag acgataagcg
     5101 ccccactcta ccgggaaact gaccttgccg gtttcgtccg ctttcagatc cagagtttgt
     5161 tcattttcaa tcagatcttt ttgatcaaac tgtgattgcc agccttcatc ttctgaccag
     5221 ttccagtagt aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt
     5281 ttcacgcctt gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca
     5341 atgggctgtt tcaccgtgct gtcagtgcga taatcgtaaa ccgatttcga ggcgaactgc
     5401 ggacggatcc ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc
     5461 gggcgaccgc ccgattccag caaactgccc tggaaaataa cctgcaatgg ggaatgcgtt
     5521 tccttccact ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga
     5581 acttcatcca gcgtgcggga aaggttttcg gcagctatat cgccgaattc aaaaccaggt
     5641 aaggctgaca cagcctcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca
     5701 ttagcaggcg caccgtacag gtagtatccc accacggaga atttcacttc atcattcggc
     5761 gtaagcgggg ttttctcacc ggtcagattc agcgccatac gctctggcat aaaatcttcg
     5821 acgtggaaat cccacatccg atactggtta tcgcccgtgt tagcgcgaat atgccacata
     5881 ccggttgccg cattgctatc gagtggccag gtaaagtggt agaggccatt ttccggctga
     5941 ctaacgacgc tcctgagtac ctgcccatcg ggtttaatca cgtctaactt gatgggttga
     6001 ttgggcaacg ctttaccgtc tgcatcacgc agcaaaccat tgaggattac cgtttcaccc
     6061 gggcgataaa gatcgcgcgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca
     6121 gcaatgttaa attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga
     6181 ccgtctttac gtgccagcaa taatgcggcg tttttatcat tttccagctg cacatgcccc
     6241 tgagcgtcac tggttgcctg agtcagagtc tgccctttct catttaataa agagacttca
     6301 attccttgct gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga
     6361 taacggtgag ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa
     6421 tcgtaacgtc cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg
     6481 atatcgccca gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac
     6541 cgtccggtgt agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc
     6601 gaattgcggt attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag
     6661 aagttaacat cgacattatt aacgttgagc gccattaccg gcagcccttc aacgacttta
     6721 ccaggcagca gcgaaccacg gctggcaaaa ccgacactgg gttggatgtc gcgggtagtt
     6781 atagtttttt cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca
     6841 attgtaacga tcaaatcacg tttcggttcg aggtggcgta aacgcagctc tttaagatta
     6901 tctgatagct cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg
     6961 cgtgagaaat cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca
     7021 ccatcaagtt ggacttcaga gagatccagc aatgtcagcg ccttaccggc actctgttgg
     7081 gccagttttt gtcgctctgg cgcggagagt tttgctgaac tcgcgttttc tgaagaggcc
     7141 gctttagtaa cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg
     7201 tcgcaccctg ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt
     7261 catccctgcc acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg
     7321 ttatgtaatt cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac
     7381 agccaaatca gcctcttttg cttgtcgctt ttcactaaac atccgacaat ttagcctaac
     7441 cccggcaaaa atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc
     7501 cgaacatatt gatgacccgg aaattcagat tatcgatgcc cgcatggcgt cgcctggaca
     7561 ggaagatcgt aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt
     7621 tgatatcgaa gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga
     7681 aacgttcgcc gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta
     7741 tgacgaaggt aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt
     7801 agagaaagtg tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgttgtt
     7861 agaagaaggt gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc
     7921 agtggtgaaa gtaaccgatg tgttattggc aagccatgaa aatacggcgc aaattattga
     7981 tgcccgcccg gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg
     8041 cggacatatt cccggtgcat tgaacgttcc gtggacggaa ctggtgcgcg aaggcgaact
     8101 aaaaacgacc gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct atgacaaacc
     8161 aattatcgtc agctgcggct ctggtgtaac agcagccgtg gttttgttgg cactcgcgac
     8221 gctggatgtg ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc
     8281 agatttaccg gttgagccat tgaaataagc tttttacagg caataaaaaa ccgccgaatt
     8341 tggcggtttt ttattgctgg tctggttcgc ggcctttcca gcaggttgac ttgtgttgcc
     8401 ggagcaacgc aggcacccca cagtgttgaa atactctctt ttatgcctgg ctgtgtcaag
     8461 ataataaaca gagtaattat tcaaacatga gagtattatt cttaatggcc cttttcgccg
     8521 tctcgcaaac gggcgctggc tttaggaaag aatgttccat gaccgtaaat acaggcaccc
     8581 cacagtgctt gctatcgcgg caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat
     8641 ggaccgcaca atgaagatac ggtgcttctg tatcgtactt attgtttctg gtgcgctgtt
     8701 aaccgaggta aataataacc ggagtctctc cggcgacaat ttactggtga ttaacaacct
     8761 tcagagcagc aggtaagccc gaatgccgcc ctttggggcg gcatatttta gattatccga
     8821 ttctgtttaa agtcacgcaa aaaaccaccc cagcgacgtt catagaatgg cgcaatatgt
     8881 tcggtaataa agtggctaat tcctttttcc ccttttttca cctgacaaat atcgattggt
     8941 tcatcgccag gtaatgtatc ggtcgctaca cttcccgcca cctgaataat ttcttcgata
     9001 tcaccatcgg cttcaatgcc aataagtaaa ttaggctgtg cctcttcgtt ctctttaatt
     9061 gaacaaataa aagcacgctt cacaggctta atggttttaa ataaggtggt gagtgaatca
     9121 atcatttgtg ctggcggctc tgcgacttcc gataatatca gcgattcacc gccttccagg
     9181 atttcctggc tgctcagcgg atttccctct tcaccaatca acaaactgat ttcacgcggc
     9241 ataaattctt taccggtcgg cagtttggca ttaaggaaga gcgtttcgcc aagggtcatc
     9301 tcaaacaatg tgcgaacggg cattacgaca aatgcctgtt cgtcttcaac cgcctgttga
     9361 agtgcttcta acgaggtaaa aaaaggaatg acgctggtgc cgtcttcttt ttcccagtgc
     9421 tgtaaatcaa gcgcgctatc ttcaaccaca gcctcgccct gcgccgccgt accaggcacc
     9481 cagacggtgg attccagtag agtacggaaa aaggccgggc ggtgcgccgg ttcggttgct
     9541 gctttttcca gcaggtcttc aagttcattt tttgtttcgg acataagaac cacaattcat
     9601 tcaacgttcg gggcacaagt gcctgatgcg ccatacttat caggcctaca agggattcac
     9661 aatttgttga atttgcaata ctttgtaggc cggataaggc gtttacgccg catccggcat
     9721 attagtttac gccgttaaca gattagcaat cgtgcgcaca ccaagtcccg tagcacccgc
     9781 agaccactgt tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc
     9841 ttgctgatag ttctcaacaa agtgcgacag gaagcccgct gccgtgctcg cgcctgccgg
     9901 atacgccgcg cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg
     9961 gaattccgcc agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagcaa
    10021 acgaccggca agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt
    10081 tttcgccgcc ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc
    10141 atcaatcaga ccatcggcaa gcaccaggcg cccttcggca tcagtgttca tcacttcaac
    10201 ttttttaccg ttgcgataag tgatgatatc gcccagcttg aacgcattgc cgctaatcag
    10261 gttatccgca cagcagagga acagcttcac gcgcttgttc agtccgcgcg taatggcaaa
    10321 tgccagcgcc ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa
    10381 cgcagtctgt ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca
    10441 cgcgtacact ggcgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg
    10501 agaacgttct gaaccgcgtc cgacggtgtg cagccccata taaccttgct cacgcagatc
    10561 ttcgcctttg gtgatccgat aagtcacacg atcgctcgcg acgttgctga tcagatcaac
    10621 agcacgctgt gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg
    10681 cacccagtcg atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc
    10741 cggccactcc actttacgcg tgcctttcgg ggctttgtaa ccttgccaga atgcccagca
    10801 tcgatccgca tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat
    10861 cttgcgcgcc gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat
    10921 gccgtcatta ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt
    10981 agagagggta atcttcatcg cttctgtcat tttagttatc cttctttgta aaagggccgc
    11041 cagttggcag ccccaggatt tttccgctaa atgattcgcg ttgcaggaag gcggcaagtg
    11101 agtgaagccc caggagcata gataactatg tgactggggt gaacgagcgc agccaacgca
    11161 tctgcggcgt gaagcatgac gcggaaatta ttcggcctcg tccagccaga ctaacaaaat
    11221 cgcttcgagg attttctcgt tggatgcctg cgggtcgtcg tcgaaatctt ccagatcgca
    11281 aatccactga tgcatatcgg tgaatcgaac cgttttcgga tcaagatcgg gatacgcatc
    11341 gtacagtgct tcgccaattt cgcggctatc ggtccactta agtcccatac taacctctgt
    11401 taatgctcac gcgcatggtt gatcgtgtaa cgcgggattt cgaccactaa atcttcgtcg
    11461 gtgacgcgcg cctggcagct taaacggctt tccggctcca gtccccaggc tttgtccagc
    11521 atgtcgtctt cctgctctga gctttctgga agcgagtcaa aaccttcacg aacgatgcag
    11581 tggcaggtgg tgcaagcaca ggatttttca caggcgtgtt caatctcgat accgttacgc
    11641 agagctgcgt cgagaatggt ttcaccgcta ttagcttcca gaacagcgcc atcagggcag
    11701 agatcctgat gaggcaaaat aacaatcttt ggcatattaa acctcgtcca cggaatggcc
    11761 tttcagcgca cgacgaaccg actggtccat gcggcgagcg gcgaaatcct gggtttgttt
    11821 gtctacgttt ttaatcgctt gttcgatggc gtcaacgtca tcgccctgcg ccacttcact
    11881 caggtgagcg gcagcatcgt caatgacctg acgttctgcg gcgcttaaca gcgcggcatc
    11941 agcagccagc gcgccgtgca gactttccag cacacgcgcc gcttctactt tttgttctgc
    12001 cagcattcgg gcttttacgt cctgctcggc atagctcatt gagtctttga tcatcgaagc
    12061 gatttcgcta tcggtcagac cgtaagacgg tttgacctga atagacgcct caacgccggt
    12121 ggatttctcc atcgccgtca cgctcaaaag accgtcggca tcgacctgga acgtcacgcg
    12181 aatatgcgca ccgccagccg gtagcgccgg aataccacgc agcgcaaaac gcgccagtga
    12241 gcggcagtcc tgcaccagtt cgcgctcacc ctgcattaca tggatagaca tcgccgtctg
    12301 accatcttta aaggtggtga aatcctgagc gcgggccact ggaatagtgg tattacgcgg
    12361 aatcactttc tccaccaggc cgcccatcgt ttcgaggccc agcgacagtg ggatcacatc
    12421 aagcaacagc atttcgctgt ctggcttgtt acccaccaga atatccgcct gaatcgccgc
    12481 gccaatagcg acgactttat ccgggtcgat ggaagtcagc ggtggacgac cgaaaaattc
    12541 gcctacccgt tcacgcacca gcggcacgcg agtagaaccg cccaccatca ccacttccag
    12601 cacttcatca gcttctacac ccgcgtcttt cagcgcgcga cgacaagcca ataaggttcg
    12661 ttttaccagt ggcgcgatca gttcattgaa ttgttcacgg ctgatttcgc cctgccagcc
    12721 cgcaacgtta acggtcacgg agtccgcatc gctcagcgcg attttggctg caatggcggc
    12781 atccagcagt tcacgctgaa cgcggttatc gctacgatca ggaatgcccg cctgctcgcg
    12841 aatgtaatcc gccagcagat ggtcgaaatc atcgccgccg agcgcggaat caccgccggt
    12901 tgccagcact tcaaacacgc cgcgacttaa gcgcagaatg gaaatatcaa acgtcccgcc
    12961 accgaggtca taaacggcga tcacgccttc ctgaccggaa tccagcccgt aggcgatagc
    13021 cgcagcggtc ggttcgttaa gtaagcgcag gacgtgaagg cccgccagac gcgccgcgtc
    13081 tttggtgccc tgacgctggg catcgtcaaa gtacgccgga acggtgataa ctacaccatc
    13141 cagctcgcct gccagggctt cagttgcccg cgccgccagt gctttgagga tgtccgcaga
    13201 aacgcgcacc gggttcagca gccccgccgc cgtttcaatc atcggcaggc cgttttcgct
    13261 ggcctggaat tgataaggca gatgcggata gcgttgctgg atatcagcca gcgagcgtcc
    13321 catcaggcgt ttaacagaac taattgtgtt ggcggtatcg agcgctgcat tagtacgcgc
    13381 gtcataaccc accgaatgcc cttgctgttg atagtgaaca acagatggca gcaggtgacg
    13441 gccttcatgg tcggctaacg tttcggcctg accgctgcgc actgtcgcca ccagcgagtt
    13501 ggttgtgccc aggtcaatac cggccgccag acgacgctga tgcggcgcag cactcaaacc
    13561 aggttcacta atttgtaata aggccatgtt tagcttccag aaattaaaaa tcgagcagtt
    13621 tttcttcgag ttgttcggca ctgcttcgca gtttatcgag aaaacgcagc ttacgcacgg
    13681 tatccgccgc cgcgtcccag gtctcgttgt ctaactgttc aaccatcaac tgatggcggg
    13741 tatcaaacat ctttttcaca cgtttgataa agctttccag ccgcgcttca tctttcgcct
    13801 gttcgatctc gtccagctct tcgcgcagct ccaactgttc catcaggaac gcggtgtcgc
    13861 gcacagtatg ctgctcgctg gcgagatcaa agccgtgcaa agaaagcaaa tattccgcgc
    13921 gcattaacgg atgacgcagc gtttgccagg cctgattaat ggttgcagat tgctgtacgg
    13981 cggcgagttg ttccgcctgg cttccgctgg cgaatttatc aggatgatac tgacgttgta
    14041 gatcctgaaa acgcaggctc agcgcctggg tatcgagttg ataacgggca ggcaagccaa
    14101 agagggtgaa gtaatccata acattctcag ggctgcgatc cgccgcgata acggcgaatc
    14161 aggtaaaaca acccccacgc gcaggcgacc acggtggggt tatcggtatg cgcatcaaac
    14221 gtggaagctt tcgccgcaac cacactcatc tttaacgttc gggttggtga atttaaaccc
    14281 ttcgttcagg ccttctttta cgaagtccag ctgcgtaccg tccagaaatt gcaggctttt
    14341 gccatcgacc acgactttca cgcctttgtc ttcaaacacg atgtcttccg gcgtcggttc
    14401 gtcaacaaat tccagtacat aagccatacc tgaacatccg gaggttctca cgcccagacg
    14461 taggccaaac cctttgccac ggttagccag aaaggtattt actcgcgctg ctgcactgtc
    14521 gctaagtgta atcgacataa ccaaacctca actcttattt tgcttcacgt ttgcttttat
    14581 agtccgcaat ggcggctttg atcgcgtctt ctgccagaat agaacagtga attttcaccg
    14641 gcggcagttc aagttcttca gcaatatcgg tgtttttgat agcctgcgct tcgtcgagag
    14701 acttcccttt cacccattcg gtgaccaggg agctggaagc gatagcagaa ccgcagccgt
    14761 aagttttaaa acgcgcgtct tcaatgatac cttcatcgtt gactttaatc tgcaacttca
    14821 ttacgtcgcc acaggccggt gcacccacca tgccgctgcc gacgttctcg tcgttgttgt
    14881 caaaggaacc cacgttacgc ggattctcgt aatggtcgat aactttttcg ctgtaagcca
    14941 ttataaattc tcctgattcc gataccgatt aatgatgagc ccattcgatg ctgttcagat
    15001 ccacgccctg cttgtacatt tcccacagcg gagaaaggtc acgcagacga ccgatggatt
    15061 tacgaactaa ctcgatggtg tagtcgatct cttcttcagt agtaaaacga cctaaagaga
    15121 aacggataga gctatgtgcc agctcgtcgt tcagccccag cgcgcgcagc acgtaggacg
    15181 gttcgaggct tgctgacgta caggcggaac cggaagaaac cgcgagatct ttcagcgcca
    15241 taatcagcga ctcaccttca acgtagttga agctgacgtt gagaatgttc ggcgcaccgt
    15301 gttccaggtc accgttcagg taaacttctt cgatatcttt gatgccgttc cacagacggt
    15361 tacgcaggcc gcgcagacgt tccatctcgg tcgccatctc ttcttttgcg atgcgatagg
    15421 cttcgcccat tccgacgatc tggtgaacag gcagagtgcc ggaacgcata ccgcgctcgt
    15481 gaccgccgcc gtgcatttgc gcttcgatgc gtacgcgcgg tttacgacgc acatacagcg
    15541 caccgatacc tttcgggcca tagattttgt gaccggagaa agacatcagg tcaactttca
    15601 actggctcag gtcgataggc agtttgccca cgctctgggt tgcatcaacg tgatagataa
    15661 tgccacgagc acggcacatt tcgccgatag ccgcgatatc ctgcaccacg ccgatttcgt
    15721 tatttacgtg catgatagaa acaaggatgg tgtcgtcacg catcgctgct tcgagttctt
    15781 tcaggtcgat aataccgtta cgctgcggtg cgaggtaggt gacttcgaaa ccttcgcgct
    15841 ccagctgacg gcaggtatcc aataccgctt tgtgttcggt tttgctggtg attatgtgct
    15901 tgcctttttt ctgataaaag ttggctgcac ctttgatcgc caggttgtca gactcggttg
    15961 caccagaggt aaagacgatt tcacgcggat cagcgccgac cagatcggca atctgattac
    16021 gggcgatatc taccgcttct tcagcctgcc agccgaaacg gtgagaacgg gaggccgggt
    16081 taccaaaggt tccgtccatc gtcataaact gcatcatttt ctcggcaaca cgcgggtcca
    16141 ccggcgtggt tgcggagtag tcgagataaa tcggtaattt cattgctcta taaactccgt
    16201 acatcactca atgcaaggaa ccaggctacc ggctggatgt acgaccgtgt ttacggagcg
    16261 cgatacgccc cggcctgatt ctgaatactt tttattaagc gcgtaactta acgtcgatcg
    16321 cgtcttgtgt gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca
    16381 cttcctggtt gttaaccagt tcgcctaaag taatgttatt gagaaaaccg gtaagacggt
    16441 cgctcaaatc acgccacagc gcgtgggtca ggcatttatc gccgccctgg cagccgcctt
    16501 taccctgaca acgggtggca tctacagatt cgtcaacggc gctaatcact tcgccaacgg
    16561 cgatgctgct ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa
    16621 ccagaccatt tttacgcaga cgagaaaaca gttgttccag ataagaaagg gaaattccct
    16681 gacgttcgga aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa
    16741 gcattgcggt cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa
    16801 actcgcccct gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag
    16861 tctgacattc ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa
    16921 ccttcagtgc cattttttta tcgtggcgtt tgtacctgtt atccttccag accatgggcg
    16981 ggttgagggc aattattcgc attagcgcag gcctgttccc ctcaccctaa ccctctcccc
    17041 gaaggggcga ggggaccgtc cgtgcactta tctggcttag ccctcaggcc ttattacctt
    17101 tattctgctg ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg
    17161 ggcgcgcacg ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac
    17221 ggataaaacc ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat
    17281 cgaccagcgg atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca
    17341 gccaggccat acgcacttca taggcaataa cctgaaccgc catcgccagg ttcagcgagc
    17401 tgtattcagg gttagcggca atcgcgacat gataatgaca cttctgcaac tcttcattgg
    17461 tcaggccgac acgttcgcga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcaa
    17521 cgcttttcag accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggaacgtg
    17581 cgctggtgcc caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt
    17641 gagcattacc aatcacatcg ctggcccccg ccgccagggc aatcgcctgg gagtcaggtt
    17701 tcaccagtgg attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa
    17761 cagaacccat attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca
    17821 ttgtctttct tcgtctaaag attattcgcg catcttatca taaaacgaag acagatgccg
    17881 atctcgctgc tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga
    17941 tgcatccgat gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttgattgcca
    18001 aaaactatga aaccccggac gctgtagaag cgagccagaa aggcagtaac gacttcgtga
    18061 ccaacgtaga taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttacccac
    18121 agcacaccat catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat
    18181 gggttatcga tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg
    18241 tatctatcgc cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc
    18301 gtaacgaact gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc
    18361 gcggcagcac cgctcgcgat ctcgacggta ctattctggc gaccggcttc ccgttcaaag
    18421 caaaacagta cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag
    18481 acttccgtcg taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg
    18541 acggtttctt tgaaatcggt ctgcgcccgt gggacttcgc tgcaggcgag ctgcttgttc
    18601 gtgaagcggg cggcatcgtc agcgacttca ccggtggtca taactatatg ctgaccggta
    18661 acatcgttgc tggtaacccg cgtgttgtta aagccatgct ggcgaacatg cgtgacgagt
    18721 taagcgacgc tctgaagcgt taatgactca ggcgggtgat atcactcacc cgccctcgcc
    18781 tttcaggcgc tattccgaaa tacttcctca ccgctttact ttctttcgtc actctcccac
    18841 catctttccc cgattaatgg atgataaaga agtcgcaagg aaaggttatg gcactgccag
    18901 tgaacaaacg cgttcccaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt
    18961 tagtgccgcg cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg
    19021 acccctggtc ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct
    19081 ggtttattcc ttcttcgacg ggccctgctg acaacgccat cgcaaccatc attcatgctc
    19141 acggcaatgc cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta
    19201 atttcaacgt ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc
    19261 aggccggatt gctggacgat acgcaaagcg ccatcaatgt ggtgcgccat cgcagtgatg
    19321 taaacccaca acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg
    19381 ttattggtca gggtgatcgt gaaggcatac gtgcagtgat cctcgactcc acatttgcct
    19441 cttatgcaac catcgccaac cagatgatcc ccggcagtgg ctacttactt gatgagagtt
    19501 acagcggcga aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta
    19561 aagctgatca cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagagc
    19621 caaaacggtt gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg
    19681 gcgacgttta tcgcgaacag atggtgaact ttatccttag cgcgttgaat ccgcagaact
    19741 aacccatgat cgctagcacg ataatcattc acaaaatcac cttaagacat gctaatccac
    19801 tggtcaggac agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt
    19861 agcattccct tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg
    19921 ggagatctac agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc
    19981 cgctgccaga tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc
    20041 agcgagctta atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg
    20101 gagatcctgc gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt
    20161 gaaggtacag tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt
    20221 ctgtgtccgt catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg
    20281 cgcggaattg cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc
    20341 tcccgacggc tgcaaaaacg ctttgaatcg cgcgatattc atttcctgtg tctgtatctg
    20401 caatattgtt tgctgcagca ccacgctgga attacgcctc agtttaatcc gctccaacgt
    20461 cgctgggcgg aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag
    20521 cgtcgggcgc tccagcctgt accacctgat gagccactgt ttatggcact acttttttcc
    20581 atgttgcggg ttcccgatcc attgcgggat gcgcatcagc gggacagaca attgcgtcag
    20641 tctatcaaac gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa
    20701 caggggttat gtgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt
    20761 gccatcggta ttgataatac cctgccggaa gagttcgcca gactgtatcc acgcctggta
    20821 cgcaccaccc gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat
    20881 gaggaaagtg gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg
    20941 catgaaaaac agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag
    21001 caacagttac gcgaactaac gttactgccg ctcaacatta aacatatgtc ggtaaaggca
    21061 tttttgcaga caggcgctcc gcgtggcgcg gcactgatta ttgctcctta taccatgccg
    21121 ttaccgctct tttcaccacc gctgatctat acggacctga cactgacaac acatcaacag
    21181 gagcagatcc gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat
    21241 tgccggaagc gccaccagcg ccatcaccca gaacacgccg tggcccagat attgatacag
    21301 gaaaccggcg aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac
    21361 cgcctgtaaa cggatgactt cgctaccctg gcgagcagca atatagcgca tggcggccag
    21421 gtggcagacc gtgaaggtgc cgcaatgcag aatttgcacc actatcaacc acggcaacgc
    21481 cgtagttgct cccataatgc cccagcgcac tacgccgcaa atcgctgaga tcaacagcat
    21541 atcgcgtgca ctacaacggc ggaaaagttt attactcagt gcaaagataa tgacttccgc
    21601 caccacgccc agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca
    21661 gtaaatggcg ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc
    21721 cagaaagcgc cagttctggc gaaccagcgc caaccacgct gaccaaccgg tgctctcctg
    21781 ctggcggctt gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga
    21841 tgccacgccc aacgtcaaca gcacgaggat cacccgataa tcaaacatag tgaccagttt
    21901 gcccgtcagc gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta
    21961 atcaagcggg aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg
    22021 tgagaaaaag aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc
    22081 ccagaaggcg acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag
    22141 gcgagaagga tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag
    22201 acctgccccc aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac
    22261 gctccagaaa ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa
    22321 ccagcgcgtg gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggagtta
    22381 atctaatgtt ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta
    22441 acgccagttc acaagcactc aaaaactgcg atccgtgacg catgttgcaa agataatgcg
    22501 taattatgcc gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc
    22561 cggcttcatc tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc
    22621 aatgggacga aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt
    22681 gacgcccagc cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac
    22741 gatatttggc tggctgtttt cttgtgcaaa ccacgcttta acgatcggcg caagcgaacc
    22801 ggaatacgcc ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg cggtgatctc
    22861 tttttcatgt gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag
    22921 ctccagataa tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc
    22981 accacggcga attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt
    23041 gtctggctgt ctgagacgaa acatcggtaa tacttttggc agtaaattca cttccgccga
    23101 tggcacaaag ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt
    23161 ggcattttcc gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc
    23221 cgtcaacgcg actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag
    23281 atcgcggatc tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt
    23341 aaagttcagt gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt
    23401 ccccttgtta tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa
    23461 ccaaccagca acatccttat gacacaaaaa tagaaggtca atacatctta tctttcagga
    23521 ttaaaaaata tgaccacacc ctcagatttg aacatttacc aactgattga cacccaaaac
    23581 ggtcgggtca ctccgcgtat ttataccgac ccggacattt accaactgga gcttgagcgt
    23641 attttcggtc gttgctggct atttctcgcc cacgaaagcc agatcccaaa acccggtgat
    23701 ttctttaaca cctacatggg agaagatgca gttgtcgtgg tgcgccagaa agacggcagc
    23761 atcaaggcgt ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc
    23821 ggcaacactc gcgcctttac ctgcccgtat cacggctggt cttatggcat taacggcgag
    23881 ttgatcgatg taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg
    23941 gggctaaacg aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat
    24001 accagcgcac cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg
    24061 ctggatcgtc gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac
    24121 tgcaactgga aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc
    24181 catgcttctg ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt
    24241 gatggacaaa ccgcccgccc ggtgtgggaa accgccaaag atgccctgca atttggtcag
    24301 gatggtcacg gcagcggctt cttctttact gaaaaaccag atgctaatgt ctgggtcgat
    24361 ggcgcagttt ccagctatta ccgcgaaacc tatgccgaag ctgaacaacg tttaggtgaa
    24421 gttcgcgccc tgcgcctggc gggtcataac aatattttcc cgacactttc ctggctcaac
    24481 ggcactgcca cactccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg
    24541 ttctgtatta ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc
    24601 actcgtgctt ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa
    24661 atccagaaat tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg
    24721 cttggtcagg aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca
    24781 gaaactgctg ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg
    24841 caggaagtgc tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag
    24901 tttcactaga gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg
    24961 acgactggaa atttcgtgac tggctggcac agctcgacga agagatttgt tacaccatgc
    25021 gcaccacggt gaacgcgcaa acgcgcgacc gccgcaaagg tgttcagccg ccgacaacct
    25081 ggatttttaa tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca
    25141 tggcatgggc agaagagccg ccgtcacgca cccgccactt aatcagcaac tgccagataa
    25201 gcgaaaccga catcccaaac gtatttgctg ttcgggtgaa ttatctgctt tatcgggcac
    25261 aaaaagagcg cgatgaaacg ttctatgtcg ggactcgttt tgacaaagtt cgccgtctgg
    25321 aagatgacaa ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt
    25381 cccataacct gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt
    25441 gccggagggt gaggctctcc ggatcgatac ctcgcccgtc atcgccttgt tcaacgttgg
    25501 cggcgagttt tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg
    25561 gtatctggaa gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa
    25621 aacggggaaa gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt
    25681 tgaaggtggt gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac
    25741 gagtccattt ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt
    25801 atcgaagaag gcgcgcaggt tgccacgctg gaactgtcgg cggcgaaagt cgccagtctg
    25861 cgtcagcgat ttggtgaaca tattctggcg gtggaaggca acgtgacctg ttatgccgat
    25921 tatcaacgcg cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc
    25981 aatgcgggca tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa
    26041 accggcttcc acgagctgtt taacgtcaac gtactcggtt acctgctggg tgcaaaagcc
    26101 tgcgctccgg cgttaatcgc cggtgaaggc agcatgattt tcacactatc aaatgccgcc
    26161 tggtatcccg gcggcggtgg cccgctgtac accgccagta aacatgccgc aaccgggctt
    26221 attcgccaac tggcttatga actggctccg aaagtacggg tgaatggcgt cggcccttgt
    26281 ggtatggcca gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcaataatg
    26341 cagtctctga cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg
    26401 gctgatttta cgggtccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc
    26461 ggtgtgatga tcaacgctga tgcgggttta gcaattcgcg gcattcgcca cgtagcggct
    26521 gggttggatc tttaaggaag tacgatgaaa gaaaaaacga tcattattgt cgggggcggg
    26581 caagcggcgg caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat
    26641 ctgttttccg atgagcaaca tcttccttat gaacgccctc cgctctcgaa atccatgttg
    26701 ctggaagatt ccccacagtt gcagtctgtg ttacccgctc actggtggca ggaaaacaat
    26761 gttcatctgc attccggtgt aaccatcaaa acattgggcc gcgacacacg agagttagtg
    26821 ttagccaacg gcgaaagctg gcactgggat cagcttttta tagcaaccgg cgcggcagct
    26881 cgaccgctgc cgttgcttga tgcactggga gaacgctgct ttaccctgcg ccatgccggt
    26941 gatgccgcca gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc
    27001 ggaactattg gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg
    27061 attgaactgg cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt
    27121 ttacagcgcc atcagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg
    27181 gtcgatggtg aaaaagtaga actgacgctg caaagtggcg agacgcttca ggccgatgtg
    27241 gtgatttacg gtattggtat cagcgccaac gaccaactgg ctcgcgaggc caaccttgat
    27301 actaccaatg gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc
    27361 ggtggcgatg tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg
    27421 gaaaacgcca ataaccatgc gcaaattgcc gctgccgcaa tgttagggct accgcttccg
    27481 ctactgccgc cgccgtggtt ctggagtgat cagtacagtg ataacttaca gtttattggc
    27541 gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaacccagaa agcgatttgg
    27601 tttaatctgc aaaacggcgt gcttatcggt gcggtaacgc tgaatcaggg acgtgagatt
    27661 cgctcaatcc gcaaatggat ccagagcggc aaaacgtttg atgcgaaaca gctgacagat
    27721 gagaacatcg cgcttaaatc actgtaacca ggataattag cgaatatctc aacgcctggg
    27781 gcgtggcgag gtaccagagt gtgtattacg tttaaatcac attatcttgc aaagggattg
    27841 gttatgaact cactacgtta ttttgatttt ggtgctgccc gccccgtttt gttattaatt
    27901 gcccgtatcg ccgtggtcct aattttcatt atttttggtt ttcccaaaat gatgggcttt
    27961 gacggtacgg tccaatatat ggcctcgctg ggcgcaccga tgccgatgct ggcagcgatt
    28021 attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt tttcacccgt
    28081 ccgctggcgg tactgtttat tttctacacg ctgggtacgg cggtgattgg tcaccattac
    28141 tgggatatga ccggcgatgc cgtcgggcca aatatgatta atttctggaa gaatgtcagt
    28201 atcgctggcg cgttcttgct attggcaatt accgggccgg gggcaatttc tctcgatcgc
    28261 cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc ggcattttat aaattacagc
    28321 cactctaccc gcaacgacat ctctgaggtt gttgattccc ccggcgcaag cgcaatgagg
    28381 tcaccccctt ccgggcgatg atggtcatcc ggcgcatggc tcatcggctc cagacagaag
    28441 aaatcaaacg catatccttt atcaaacgcc gggtcggaaa caaagatgaa ataacacggt
    28501 gcaggtggcg tcgtttctat gatgatggca taaccctcct gcggctgctc gatgcgggcc
    28561 tgaccattcc atccggcaaa accattgttc acccactggc gcggcaacgg cgctagctgg
    28621 ttaaaatcca gttcttgcgg tagctgctcg caaaactcac ccgccagcca ctgctcccgc
    28681 tccagccagt aaccgctcgc ctgtgcctga atccgcgttt gcggcgacaa cggaaaataa
    28741 ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt gattggtgac agagagcgtc
    28801 accgtcagcg tatccgccgt taaatgaaac gcctgactta cccgatagtg atagacaccg
    28861 ctgcgatgtt catacaccag acacaaacta tcatcgctgt gcgagacaca ttgccactcg
    28921 cccagccagc catcgccgtg tagatagtgc gcatcccact caacattcgg ttgcagttga
    28981 tactcacgcc cctgccagac aaaacggttg ccgctcaccc ggtttgcaaa tggcaccagc
    29041 ggaaaacaag aggcatcggt tgccacaccg ctttttttac caggacgtaa gagcggcgtc
    29101 gtatcgcgcc agaagccttc gattacgccg ccctggtcag aaacgcccag ctttagcgac
    29161 ccgtgggata aggtatagat ggtcatccgc taactcctta atccgggaag ttaatcacaa
    29221 ctttcccgca tttgccgctc gccatcagcg cataggcatc acctgcctgt tccagcgaga
    29281 agcgatgggt aatggcgtta cgcggccaca gcttccagtc agtcaaatcg tgggcgcatt
    29341 tttccatatg gaacagactg gtcacccagg agccaataat ccgccgttga tggtgcatca
    29401 gatcggcgct gacctcgaat tccacttttc cggtttcacc aatgtaaacc acccgccccc
    29461 agtcagcggt ggattgcagt gccagtaagc gacctgcggc attaccggaa caatcaagcg
    29521 caacatccgc gccgccgtga gtgagttcgg cgattatctg cggcagacct tcagtggtcg
    29581 ctaaatagcc gtgatccatc actcctaact gttttgccat cgccagacgt tccggcagca
    29641 tatcaacgcc gataatccgt tttgcaccgc gacctttcgc cagcatcatc cccatcatgc
    29701 cgactggccc cagaccgacc accagcacgt tatcactgcc ggaaacttcg ccgcgcaaaa
    29761 tcccttcata ggccgtgcca acgccgcaac tgataaacgc accatcttcg tagctcagcg
    29821 catccggcag gaggatcaga tctttttctt ccgccagcaa gtattcggca tgaccgccgt
    29881 cacgctgcca gccgtaagcc gcttttcctt cgccagtaca agaaatagga aaaccgcgac
    29941 ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg tcgccctctt
    30001 taaaatggcg gcagccttgc cccatcgcca caatctgccc gcacggttca tgaccgttga
    30061 taaatcccta gtacaacggt ttatcggatg ccgccgccgt agcgcggtgc tggtggtaga
    30121 tatagtggac atcgcttccg caaattccgg aggatttcat tttgatcagt acctggttaa
    30181 tccccggcgt cggcactgca acttcccgca gatcgacggt cgaatttcct ggtaaataag
    30241 ctgccagcat cgttttcata aatccctcat taacaatacg attaattttc atccctgccg
    30301 cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctgctacgct gggtcagcaa
    30361 gatattcgcc agcaccgcca ccacgatgat gacgccgcgc accacctgct ggaaaaagga
    30421 gttaatcccg agcagcacca gaccgttacc gattagcgta atcaccagta cgccaagcag
    30481 cgtaccgaac aacgagccgc gaccaccgga aagcgccgta ccgccgacca cgaccgcggc
    30541 gatgacgtca aactccagac cgtttgcggc acctgcgtta ccggaaccga ggcgcgccgc
    30601 caacaaaatg ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa gaatgcgtac
    30661 ccgacgaacg ttaataccgc acaactgcgc cgccgtggca ttaccgccaa cggcaaaaac
    30721 cgagcgcccg aaggcggttt tgcggctaat gaacacaaac agcgcaaaca gcaccatcat
    30781 gatcagcgcg gataccggca caccgagaaa ttgtccgccc agccagtcca gcacctcgtt
    30841 ttcgtcaatc ggcactggca gagcgttggt cataaacaag cccattccgc gcagggcgct
    30901 ccacagcccc agcgtggcca cgaaacttgg cacgttaaac acgccacgca gcaccccggc
    30961 gagcgttccc atcagcgcgc ctaacagcaa caccagcaga caagcaatcg ccaacggaac
    31021 ttcaaattgc agcaaaaatg ccaggcatac cgagacaaaa gccaccatcg gcccaacgct
    31081 gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcaa tcccaatggt
    31141 ggcggcatcg cgcagcacgt tcatctggtt attcaatgag ataaagccag gcgcgttcag
    31201 ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat taatatggtg
    31261 actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg acgaagcaga
    31321 catcttcagg ttcctcggta aaattcatca gtgcacagac agaatggcgg acatcagctc
    31381 atccacattg accggagcgt gaaactcctg cgagaacgtg ccgtgctgta ataacaggat
    31441 gcggtcacac accaacggca actcctccac ttcactggag ataaacacca cgctttttcc
    31501 tacggcagcc agctcacgga caatacggta aatctgctgt ttggcttcga tatcgacgcc
    31561 gcgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac gaccgatcac
    31621 tactttttgc tgattgccgc cagaaagcgt gccgatgggc gtttcgctac tggcggcctt
    31681 gaccgtcatc cgctgcatca cttcttcggt caggcggcgg atggtggacc attgcagcac
    31741 tccgttggcg ctgatttttt gccgattggt cagcactgta ttttcgtcaa cgcccaacca
    31801 gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc gtttcagcat
    31861 gtcgccgtaa tcggggcgcg tgattttctc gccgttgata acaatttcgc cctgttcata
    31921 cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca gcagaccagc
    31981 aatgccgagc acttcgccac gacgtagcgt aaagctgata tcctccagct tgggcttatg
    32041 gcgtaacgca cggacttcca gcacggcctg atccacaatt tcctgaggtg caaccggcgc
    32101 aatatcaacg tgatcgcgcc ctagcattag cgacacaata tgatgcgtgg aagtgttttc
    32161 gagcatcaca tcgccagcca cctgcccgtc gcgcataacg gtggcactgg aggcaatgcg
    32221 gcgaatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg ctgacatctt
    32281 tttcaccgct ctgatcacca gttcaacttc cgcactcgca agcgaactgg taggttcatc
    32341 aagaatgacc acgcgcggct cgcccttcat cacccgcgca atttccacca gctgcttatg
    32401 cgccgggctt agtgttgaaa caagttgttc aggactaacg tcaacgccca gcgcctgtaa
    32461 gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc ggcggggcca
    32521 ctgaccgagg cagaggtttt ccgccaccgt cagcccttcc accagactta actcctgata
    32581 aaccgcgcgt acccccagtt cagcggcacg gcgagtcagc gtagcttcgt caccttccag
    32641 tcgcgtctcg ccaatccaga tttcaccgct atccggacgt tcactgccgg taagcattcg
    32701 aatgagggtc gatttgcccg cgccgttttt gcctaacagc gcacgaacct cgcctttatt
    32761 gagcgtgaag ttaacgttat ccaacgcaac gacgccggga taacgcttat ttcctgccac
    32821 cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg cgaatcagcg
    32881 attagggcag accatcaacg tgcgttgcca gccactgttt accgtcttcc gttttggtat
    32941 agagatcgat aggcacctga atcacttttt caccgtcggc ttgtttattg ataaccttca
    33001 atgtttgcgc gaaaacagca ttgcccattt tcttaccgga aatatccact accgctttca
    33061 gcacctgatt gttttccagc tcctgagcaa tttcggttgt catatccgaa ccgaaaacag
    33121 caatttttcc ggcctgattt tgattacgta ccgcttttac cgcgccgagt gtcgcaccgc
    33181 ccgattcccc cataatggcg ttgagatccg gcgtggagat aatcagtttt tcaccaacgg
    33241 aaatcgcttt atctaaaaca gtcccttcct gattagcgac aatttgcgcg ccgggaacgc
    33301 gggattttaa tacttcttca aatcctttac gtcgctgcac acaaacttca aaggcttcgc
    33361 aattgatgac ggcaattttc ggctggtcaa ttttattggc aataaaataa tcggcggcag
    33421 cgttacccag ttttttacca aattccagcg ggtcacccac cagatacgcc gagacatatt
    33481 tatcgacccc cttttgatta atacaggtgt tgtagcaaat caccggaata cccgcttcac
    33541 tggcgcgacg aacggtacgg ctactgccat tttcagacac tgccgataaa ataatagcat
    33601 cgacattacg cgccacgagg gtatcaacaa aggtactttc tttcgaaata tcaccctggg
    33661 cgttagtttc aattaactgt acctgtactg aagaatcttt tgccgcatcc tgaactccct
    33721 gacgcactcc agcgtaatat ccctgagtat caaggtatat tgcgccaatg gtcatttctt
    33781 tatccgcagc tctggcaaat agtgcgctac ctaatagcgt agccattaat aatagattac
    33841 gggttgttct cattttttta ggcatagagc ctcctgtagg gtttttatta acaacggctt
    33901 attctaatta ttttgtgatg agcggcagcg cgtgccgctc aaatattaca gttaataggg
    33961 tttagtgaac gtagttaaaa ataaaaggca tcacggtggt gaataatgcc gctttcggcc
    34021 aggccggatt aatcgccgtc aattcagcgc gagcagattc gtattgcgca atctcgccta
    34081 atcccgcggc cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat
    34141 cgccatacag cgacaacaag tcaggctgcg agacggcaaa gaaatccgct tcgatactgg
    34201 ttttcgccat ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtgtgtt
    34261 gttcgcccag taatcgcagc gccattcctt gccagaagag gtaatcaacc ggctgatcgt
    34321 tgtaatagct gtggatgtta atggtgcgat cgccggtcgc cgccagatgt aaacagcgcg
    34381 tcgcttcagt ttcatcgccc tgggctttgg cgcatatcgc ctgccagaac cagatgtcgt
    34441 tatcagtttg ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat
    34501 gaagcagttc gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata
    34561 actggttgag gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt
    34621 tgcgcgtggc gagaatgtcc gccgctttgt ctgcctgccc cgtgagatgc cacaaattga
    34681 gcagttctgc ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg
    34741 ccagtcgttt ctccggtgtg gttccactta gcttatcaag caaatcccgt tcgaaaagca
    34801 gacgtgcatc ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat
    34861 aatcgtgttg cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg
    34921 gcgacatctc tacgcaacgt tgccataagg caatggcttc gttgtagcta cgtttgttgt
    34981 agtagaagca ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg
    35041 ccacttcttc cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg
    35101 cgaccagttc gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg
    35161 tgcgctggca gtccagtgca ttcagcatct ctgccgcgag ggtgggcatt ccccagttaa
    35221 tcagttgccc ggcggtcatc agggcgttaa cgtcgcgtcc ctgacacaga ccgcgccact
    35281 gcgcgagagc tgattcgcta cgaccatcga accagttcag gcaccacaga gtggcgttca
    35341 gcggataatc gcgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta
    35401 acaccagcag cagattatgc aggcaaagca cttcctgatt gattgggcag gcgcgaagac
    35461 tttgttggca aaaatccaga cccgcgtcga agttaccatt acgcgccgcc agtcgtgcca
    35521 gaccataata gcctccggct ttactgttgc cgctccagac cgcacgccag aaatcctctt
    35581 cggcttgttg atattgtccc tgacgttcgt aagcactggc gcgaatcaaa ctcgcctgtc
    35641 cgcactgcgg atttttgttc agcgcatgtg cgcgtttcag agcctgagtg gcatacgcca
    35701 ccgcttgcgg gaaatctgcg cggttatact ccagcatcgc cagcgccaga ttacagcggt
    35761 aatccagcgg atccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt
    35821 gatgatattg ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt
    35881 gtgctgacag tggcgctttg gcgacgtccg gcaacggcaa ctcttgcggc tgatgttcct
    35941 gataactcag tacaatattg ccgtcggcat cagagagctc aatggtcagt ctttcaggat
    36001 tgataccgtg taacacgccc tggatggcgg tcgctggtgt cagggccacg gcatcatcga
    36061 gcaacgcgtc gcatttgccg atttcgcgga tcgccaggcg gtatccgttc aacggagaga
    36121 tggcatacag cccccactca atcccccgct cactacgctg gagtttgatt accgcatcgc
    36181 gggaggcgtt ttgcaccatg cccagagaat ggtaaggcag gaaaaactgc tcaaaacgct
    36241 tctcttcgta agcatcaagc cgggtaaaat caggctggtt atcggcaaaa ataccggtca
    36301 tcagttcgat atacgggccg ttattatcag ttaggctctt atcccacgcc tggccaaatt
    36361 cactgtgtcc ccagctccac tgctttttac ctggcgcaat atggtggttg gcaacgtgta
    36421 gcaaaccgcc atcttcatcg tgacaccacg cgccaacaaa atcgtactgt gatttttcag
    36481 ccatatatga ggttggaacg ggcacatttt tatagcgaga aatgtccact ccagcggagt
    36541 agtccacttt gtagtaagtg ccggtggcga tagggaaagc ggagacggcc cgtttgccgt
    36601 gatcaaacac cgccgttaca tccggcggaa agacgctctg atgcccttca ccccctttca
    36661 ctgccgggtt ggcccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcggc
    36721 tggcgatttc cagcgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac
    36781 catgcatcgg ctccgtttcg cctacccaca ccgtctgtgc gccgtcttca tgggcttcga
    36841 gggtgaaatc aacgggcata aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa
    36901 tcccgccgga gatccacggt cccagcagcc ccaccagcgc aggtttaatg acttcattgt
    36961 gatagacaaa atcgcgctgt ttcactttat cccatgcgcg atgcacccga ccgcccaatt
    37021 ccggcaggat catcactttg atgtagtcgt tttccagcca caccgcctgc caggatttca
    37081 gggttttctg ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc
    37141 cctgataaac gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg
    37201 tcgggatctc aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa
    37261 caagacatac tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg
    37321 cggaataatc acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa
    37381 tcaacagatt cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg
    37441 cgccaataaa tcaacgctgg cccggctggc gcaaatttcg attccggcag tcagtaatat
    37501 tttgcaggaa ctggaaagcg aaaaacgggt ggtgaatatc gacgatgaaa gccagacgcg
    37561 cgggcatagt agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgtctgaa
    37621 cgtgacgccc accagtattg agtgtcaggt tgctaatgct tgtttaagtc cgaaaggtga
    37681 atttgagtat ttacagattg atgcaccgac gccgcaggcg ctgctgtccg aaatcgaaaa
    37741 atgctggcat cgccaccgta aattgtggcc ggaccgcacc atcaatctgg cgctggcaat
    37801 ccacggtcag gttgatccag tgactggagt gtcgcaaacc atgccgcaag cgccgtggac
    37861 aacgccggtt gaggtgaagt atctgctgga agagaagctg ggcattcggg tgatggtcga
    37921 taatgactgc gtgatgctgg cgctggcgga aaaatggcaa aataattcgc aggaacggga
    37981 tttctgcgtg atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat
    38041 ttatcgcggc agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg
    38101 cgtcgtctgc gactgtggac gttacggctg cctggaaacc gtcgcctcgt taagtgcatt
    38161 aaaaaaacag gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttgatcctga
    38221 aaaactgact acagcgcagt taatcgctgc ctggcaaagt ggagaaccgt ggatcaccag
    38281 ctgggttgat cgctctgcca atgccattgg tttgagtctg tataacttcc tcaacatcct
    38341 caatattaat cagatttggt tgtacggtcg cagttgtgcc tttggtgagc actggcttaa
    38401 tactattatt cgccagacag gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa
    38461 agcgacgcaa attggcttcg ggcaattaag ccgcgcacaa caggtgctgg gaattggcta
    38521 tttgtatgtt gagacgcagt tacgacagat ttgatggcgc gataacgtag aaaggcttcc
    38581 cgaaggaagc ctttttcaac agattaaccg tttcgcttat gcgtaaaccg ggtaacgtgc
    38641 gcagatgtcg agaactttac ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc
    38701 cagcacgtca cacatccagc cagccagttc tttcgcttct acttctttga agccgcgacg
    38761 cgtaattgcc ggagtaccca cgcgaatacc ggaggtcaca aacgggctct tcggatcgtt
    38821 cggtacgctg tttttgttga cggtgatgtt agcacggccc agagcggcgt ctgcttcttt
    38881 accggtcagg tttttatcga ccagatcaac caggaacaga tggttatcag taccgccgga
    38941 aaccactttg tagccgcgtt ccaggaacac ttctaccatc gctttagcgt ttttagcgac
    39001 ctgctgctgg taagttttga actcaggctc catcgcttct ttcagagcaa ccgctttacc
    39061 ggcgattacg tgcatcaacg gaccgccctg accaccaggg aaaacggcag agttcagttt
    39121 tttgtacagc tcttcgctac cacctttcgc caggatcaga ccgccgcgcg gacccgccag
    39181 ggttttgtga gtggtggtag taacaacgtg agcatgagga accgggttcg ggtagacgcc
    39241 agcagcaacc aggcccgcaa cgtgcgccat atcaacgaac aggtaagcgc cgatgctgtc
    39301 agcgatttca cgcattttcg cccagtccac cacgccggaa tatgcagaga agccaccgat
    39361 aatcattttc ggtttgtgtt ctttggcttg tttttccaga tcggcgtagt cgatatgacc
    39421 ggtagcatca ataccgtaag gaacaatgtt gtacagttta ccggagaagt taaccggaga
    39481 accgtgagtc aggtgaccgc catgcgccag gttcataccc agaacggtat cacctggttc
    39541 cagcagcgcg gtgtagaccg caaagttagc ctgggagccg gagtgcggct ggacgttagc
    39601 gtagtcagcg ccgaacagtt ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac
    39661 atactcgcaa ccaccgtagt agcgtttgcc cggataacct tcagcatatt tgttggtcag
    39721 ctgagaaccc tgcgcctgca ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc
    39781 gatgtgctct tcctgacgta ctttttcctg ctccatagcc tgccacagtt cggcatcata
    39841 atcggcaatg ttcatttcac gctttaacat ccgcatctcc tgactcagct aacaataaaa
    39901 tttttggcct ttataggcgg tcctgttgga caacggcgaa cagtataacc gaatcattgt
    39961 gcgataacag gtcttgacaa aggaatttac gcaaacgatt accttcaggc tacgcaaggc
    40021 tttggagaat aaagagcttg caaccggaaa cggatttctt ttcaggtttg tgatgcaaat
    40081 ttttcacttc atcacattct ttctgaaaaa caccaaagaa ccatttacat tgcagggcta
    40141 ttttttataa gatgcatttg agatacatca attaagatgc aaaaaaagga agaccatatg
    40201 cttgacgctc aaaccatcgc tacagtaaaa gccaccatcc ctttactggt ggaaacgggg
    40261 ccaaagttaa ccgcccattt ctacgaccgt atgtttactc ataacccaga actcaaagaa
    40321 atttttaaca tgagtaacca gcgtaatggc gatcaacgtg aagccctgtt taacgctatt
    40381 gccgcctacg ccagtaatat tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc
    40441 gcgcagaagc acaccagttt ccagatcaaa ccggaacagt acaacatcgt cggtgaacac
    40501 ctgttggcaa cgctggacga aatgttcagc ccggggcagg aagtgctgga cgcgtggggt
    40561 aaagcctatg gtgtactggc taatgtattt atcaatcgcg aggcggaaat ctataacgaa
    40621 aacgccagca aagccggtgg ctgggaaggt actcgcgatt tccgcattgt ggctaaaaca
    40681 cctcgcagcg cgcttatcac cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca
    40741 gaataccgtc cggggcaata tctcggcgtc tggctgaagc cggaaggttt cccgcatcag
    40801 gaaattcgtc agtactcttt gactcgcaaa ccggatggca aaggctatcg tattgcggtg
    40861 aaacgcgaag agggtgggca ggtatccaac tggttgcaca atcacgccaa tgttggcgat
    40921 gtcgtgaaac tggtcgctcc ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca
    40981 gtgacgttaa tctctgccgg tgttggtcaa acgccaatgc tggcaatgct cgacacgctg
    41041 gcaaaagcag gccacacagc acaagtgaac tggttccatg cggcagaaaa tggcgatgtt
    41101 cacgcctttg ccgatgaagt taaggaactg gggcagtcac tgccgcgctt taccgcgcac
    41161 acctggtatc gtcagccgag cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt
    41221 ctgatggatt tgagcaaact ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc
    41281 tgcggcccgg ttggcttcat gcagtttgcc gcgaaacagt tagtggatct gggcgtgaag
    41341 caggaaaaca ttcattacga atgctttggc ccgcataagg tgctgtgatt tgatgttgcc
    41401 ggatggaaac atccggcaac ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg
    41461 gtacggatgc gaatgacccg tgccacgtca aagacgaaga ttttaccgtc accgattttg
    41521 ccggtttgcg ccgtgcgaat aatggtatcg acacaggtat cgacaatgtc gtccggtacg
    41581 acaatctcaa ttttcacttt cggcagaaaa tccaccatat actccgcgcc gcggtacagc
    41641 tcggtatggc ctttctggcg gccaaaacct ttcacctcag tgaccgtcat gccggtaatg
    41701 ccgacttcag ccagtgcttc gcggacatcg tccagcttga agggtttaat aatcgcatca
    41761 atctttttca tgctattcct tgaaaaggtc gcctgtcttt tgatctgcta aacgtaacac
    41821 ataacgccaa ttcattcctt gaaatcgttt gcatccagct cgtgtcggga aagcagttta
    41881 taaaattctg tccggttgcg ccccgccatt ctcgccgcgt gggtgacgtt gcctttggta
    41941 atttgcagca gcttacgcaa atagttgagt tcaaactgat tacgtgcctc aacaaaggtt
    42001 ggcagcgccg tattttcacc ctccagcgcc tgctctacta atgcatcgct aatcaccgga
    42061 gatgaggtca gcgccacgca ctgttcaatc acgttgacca actggcgcac attacccggc
    42121 cagctcgcgg tcatcaggcg tttcatcgca tcggtggaaa acgcgcggac aaacggttta
    42181 tgtcgctctg ccgcctggcg caacaggtga tttgccagta gcggaatgtc ttctgtgcgc
    42241 tccgccagtg ccggaatttt caggctgaca acgttgaggc ggtaatagag atcttcgcgg
    42301 aattccccgc gcgccatcgc ttttggcaga tcacggtgag tggcagaaat aatccgcaca
    42361 ttgatatcaa tatcgcggtt actgcccagc gggcgcactt tacgctcctg caacacgcgc
    42421 aacagtttga cctgcaacgg cgcaggcata tcgccaatct catcgagaaa tagcgttccg
    42481 ccttccgccg cctggaataa accttcgcga ttgctgacag cgccagtaaa cgcgccacgc
    42541 gcatgaccaa acagctccga ctccagcaat tgctcaggca atgcaccaca gttaatagca
    42601 ataaatggtt tgctattgcg cgggctggcg ttgtggatag cctgggcgaa aatctctttc
    42661 ccggtgccgc tctggccatt aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc
    42721 tgttccagca aacgcagcat cagcgggctg cgggtgacaa tcgcctcgcg ccagcgttcg
    42781 tcggtggctg gcgcggattg ttccagcgca tcatcaattg cctgatatag cgcgtcttta
    42841 tcaacaggct tggtgaggaa actaaaaacg ccctgctgtg ttgcagcaac ggcatcggga
    42901 atagaaccat gcgcggtaag aataattacc ggcattcccg gctgcacttt ctggatttca
    42961 gcaaacagct gcataccgtc catttcatcc atccgcaggt cgctgatgac taaatctact
    43021 ttttcgcgat tcagtacccg taatccttca gcgccacttt ccgccgtgac cacgctgtag
    43081 ccttcgctgg tcaggcgcag gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat
    43141 aataaatgcg caggtttatg gctcatcagg agtgacctca tgggtggatg gcgcgggctt
    43201 ttcactctcg tgtggcgtat ccggcgagaa atttccagcc ggtttgcggg tcgagagctg
    43261 gcgttcaata tcagtcaggt tttccagctt gcgggtggtg agttccagtt gctgctgtag
    43321 aacgtgatgt tgctggcgca atgtatccag ctcgctgtcg ctggactgct ggagtttact
    43381 gtagcgttgg cgctcttccg ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg
    43441 atacagtggg cgaacctgtg ccgggatctc ggtacttaac gcttcaatac gcgcgaccag
    43501 ctggcggcgc tcatatggcg taattttggc gtcggcgagc aaaatccctt gtttaaaggt
    43561 attttgccag ctgccgtcgt catattggcg agcttgctga cgcgactgcg caggcattaa
    43621 acgatcagca caatccatcg cccgcagcca gtaaagcgga ttggtttcgg ttgatttacc
    43681 ttgcagcgcc cagatgtcgc tacattcagt agaaagatag tcagccagtt gataaaccgg
    43741 aattttttct tctgctggcg tatcaatggc tggcttattg tgattctgca cgcaacctag
    43801 caatgccaga catggcagcc ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat
    43861 gtgtcgcata ttcaccagac ttaaagccta tcccagtggg cgtaattgtt gcagacagtt
    43921 tggacatgga cagcgcggag aaaccggagc gtacatgtcg tacgtgagga tttcgagcac
    43981 tgcccggggc cgaactgaca aataaaatag cctgatggga taggctgtta gcattatttc
    44041 gtgtttttcg acgacggtaa ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc
    44101 agatacagtt ccccttgcat acggcgaata caatccctgg caatgcttaa tcccagaccg
    44161 ctgcccttca ccgccccttt tcgctggtgg cttccctgaa aaaagggttc gaagatcatg
    44221 gcgcgttcct cttgcggaat gggcgtgcct gtattgatga catcaatata aacccgcgca
    44281 ccatgtaaac tgctgcgaag gcaaatgtta ccggattcag ccccgtagtg caccgcattg
    44341 gagtaaagat tatccagtac gctcatcagc agcattggct ccgccaggca agctgttgct
    44401 ttgagatcga cgtcggtatg catcatttta gcccgtgcgg gcaggctatg agcagaaact
    44461 actgtctcca ccagcggtgc taactcaaca ttatctagtt ccaccgcact gtccgcctgt
    44521 ttacggttgt aatcaagcag ttgttcgatc agtttttgca aattgcggct gctgctatca
    44581 agaatgctca ccacctcttt ttgctctggc gtaagcggcc cgacaacctg atcagccagt
    44641 aattcagtgc cctcgcgcat actcgccagt ggcgttttta attcatgaga taaatgtctt
    44701 aaaaattgat ggcgttggga ttccagccat gacaggcgct cacttaacca aagaatacgt
    44761 tgcccaaccg agcgtaactc gctcggtcca ctgaacgaaa cgctattgcc cagagaacgc
    44821 ccttccccca accgattgat catccgttca atatttttca ccggcccgat aatcatccgc
    44881 gtgaaaagca gtaccattac cagactcacc agaaatagca ccagcgattg ccaaccaaaa
    44941 tattgcccac gttcggcgat ttcacgctga agttgctgcc cacgagagaa cactactgtg
    45001 cgcgtggcct gtaccatttc ggtattggca ctggcaaagg cttccagacg cgcggcagca
    45061 gcagcatcgg gaccactgtt gttgcactga agttgggcca gattgttcaa gtcctgacgc
    45121 aatgcctggt aaagtttatc gtctggcagt acgcctgcgt gggcatcgag catttcgctg
    45181 taacgcttgc gctggctttg ataaaccttc gcaagcgtag ggtcatccag cacgcaatac
    45241 tgacggtagc tacgctccat ctccagcgcc gcgttggtca ttgcttcgct gcgccgggca
    45301 tcgataagcg tggtgcggtt gaccagcgcc gcctggtcgc taagcgcatt caggctttgc
    45361 caggcttgcc atgccagcac caacaggggc agcagaatca gcaaaaatgc cagcattacc
    45421 agttgtcgta atgagcgggg aaaaacgggc cagcgtttca aggtgttact ctcgtcagac
    45481 gcgaatagcc tgatgctaac cgaggggaag ttcagataca acaaagccgg gaattacccg
    45541 gctttgttat ggaataaggc ggtgcctaac tcgacgtttc gcccgatggt tgatatagct
    45601 acgctgatat cagaagttgg acggcaggca ccttgttgtg cgtcattcga attttatgta
    45661 gcacgtcccg aaggggctga cataagtcgg tgaatgagcc actgattact attatgcagt
    45721 aactgtgcca ataaagaaaa tagtttggta acgtactgat tatacgttgc tttgaggggt
    45781 ttatggcttc cccgctattt gaatgtaaat cagccactgt gtcgctaaaa agagacaact
    45841 taagaaaaac ttattagatt acaataaaat caatcattta agtgtcgcca gaaagcgaca
    45901 cggtaaacca cccattgtcg tgatttacag acacaaaaaa gctcccgaag ttgggagctt
    45961 ataatagcgg ttgtatttaa tgccggatgc ggcgtgaatg cctcatccgg gctgcaaaac
    46021 caatgagttt gacggcttac cccaactgct tacgtgcatt gcggaaaatg cgcatccatg
    46081 ggccatcctc gccccagttt tcaggatgcc aggagttgct gacagtacgg aaaacacgtt
    46141 ccgggtgcgg catcataatg gtgactcgac cgctttcagt cgtgaccgcc gtaataccgt
    46201 tcggagaacc gttcgggtta gccgggtagg tttcagtgac tttgccgaag ttatcgacat
    46261 agcgcagtgc caccagccct ttgctttcca gtgccgccag atgcgccgca tcacgcactt
    46321 ccacgcgccc ttcaccatga gagacagcaa tcggcatttg cgagcccacc atcccctgca
    46381 acagcagcga cgggctttgg gtcacttcaa ccaagctgaa acgcgcttca aagcgatcgg
    46441 aggtattgcg cacaaaacgt ggccacaact cactacctgg gatcagttca cgcagattag
    46501 acatcatctg gcaaccgtta cataccccca gcgccagcgt ttgcggacgg tggaagaagg
    46561 ttgcaaactc atcgcgtaca cggtcattga acaggataga cttcgcccaa ccttcaccgg
    46621 cacccagcac atcaccgtag gagaaaccac cgcacgcgac cagggcgtgg aaatcctcca
    46681 ggcccgtgcg tccagccagc aggtcgctca tatgcacgtc gatagcatcg aagcctgcac
    46741 ggtggaaagc tgccgccatt tcaacatgcg agttcacgcc ctgctcacgc agcacagcaa
    46801 ctttcggacg tgcgccagtg gcaatatacg gtgctgccac atcttcgttg atatcaaacg
    46861 acagtttcac attcaggccc ggatcggcgt cgtttgattt cgcctgatgc tcctgatcgg
    46921 cacactccgg gttgtcacgc aggcgctgca tctgccaggt agtttctgcc caccagacac
    46981 gcaacgtagt gcggctttcg ctgaatacag tctgcccgtt ggcggtaatc acaaaacggt
    47041 caccggaaac cgcctgccca acataatgga cgcaatcagc aagcccatgc tgtgccagta
    47101 cggactcgac cgcttcacgg tcagcggcac gaacctgaat gaccgcaccc agttcttcgt
    47161 taaacaacgc cgccaggcga tcgtcaccca gagtggcgat atccgcgtca atgccgcaat
    47221 gaccagcaaa ggccatttcc gccagcgtta ccagcagacc gccatcagag cggtcgtgat
    47281 acgccagcaa cttacgctgt gcaaccagcg cctgaatcgc gtcatagaag cctttcagtt
    47341 gcgcgacatc gcgtacatct gccggtttgt cgccaagctg acgataaacc tgcgccagcg
    47401 ccgttgcgcc cagcgcgtta ttgcctttgc ccaaatcaat cagcagcagt gcgttatctt
    47461 cggtagaaag ctgcggcgtg atggtgtgac gtacatcttc cacgcgggca aatgcagaaa
    47521 tcaccagcga cagcggcgac gtcatttcgc gctcttcgtt accttcctgc cagcgggttt
    47581 tcatcgacat agagtcttta cccaccggga tcgtcaggcc cagcgccgga caaagctctt
    47641 cgcccacggc tttaacggct tcatacaggc ccgcatcttc accagggtgg cctgccgccg
    47701 ccatccagtt ggcggaaagt ttaatgcgtt tgatatcgcc aatctgtgtt gcggcaatat
    47761 tggttaacgc ttcaccgacc gccagacggg cagaggcggc gaaatccagc agcgcaaccg
    47821 gcgcacgctc gccaatcgcc atcgcttcac cgtagtagct gtcgaggctg gcggtagtga
    47881 ccgcgcagtt agcgaccggc acctgccacg gccccaccat ctgatcgcgc gccaccatgc
    47941 cggttacgct gcggtcaccg atggtcacca ggaaagtttt ctccgccacg gtcggcagat
    48001 gcagtacgcg tttcaccgcg tctgcaatgg tgatcccttc acggaccagc gcatcgccct
    48061 tcgctttcag cgtttgtaca tcgcgggtca tcttcggcgt tttaccaagc aggacgtcca
    48121 gcggcagatc gatcggctga ttatcaaaat gacgatcgtg cagagaaaga tgcagttctt
    48181 cggtcgcttc accaatcacc gcgtagggtg cgcgctcacg cttacacagt tcgtcaaaca
    48241 gcggtaattg atcggcagca accgccagca cgtagcgttc ctgggattcg ttacaccaga
    48301 tttccagcgg gctcatgccc ggttcgtcgc ttagaatatc gcgcagttca aatttaccgc
    48361 cgcgcccgcc gtcgctcacc agttccggca tcgcgttaga aagaccgcca gcgccgacgt
    48421 cgtggataaa caggattggg ttggcatcac caagctgcca gcaacggtcg atcacttcct
    48481 ggcagcgacg ctccatctcc gggttgtcgc gctgtacgga agcaaagtcg aggtcggcat
    48541 cagactgacc tgacgccatg gaagacgccg ctccgccgcc aagaccgatg ttcattgccg
    48601 ggccgccgag aacgaccagt ttcgcaccga cgttgatctc gcctttttgt acgtgatcgg
    48661 cgcgaatgtt gccgatcccg cccgccagca tgatcggttt gtgataaccg cgcagctctt
    48721 cgccgttgtg gctgttcact ttttcttcat aagtacggaa gtagccattc agtgccggac
    48781 gaccaaattc gttgttaaac gccgcgccgc ccagcgggcc ttcggtcatg atgtccagcg
    48841 cggtgacaat gcgctcaggc ttaccgaaat cttcttccca cggctgttcg aagccaggaa
    48901 ttcgcaggtt ggaaacggag aaaccaacca aacccgcttt cggcttcgcg ccgcgcccgg
    48961 tggcaccttc atcgcggatt tcaccgccgg aaccggtcgc cgcgcccggc cacggagaaa
    49021 tcgccgtcgg gtggttgtga gtttcgactt tcatcagaat atgcgccggt tcctgatgga
    49081 aatcgtagcg gcccgtttcg tggtcagcaa agtagcggcc cacttcagaa ccttccatta
    49141 cggcggcgtt atctttataa gcagagagaa cgtgatctgg cgtggtttcg aaagtatttt
    49201 tgatcatctt gaacagcgat ttcggctgct gttcaccgtc gataatccag tcggcattaa
    49261 aaattttgtg gcggcagtgc tcagagttcg cctgagcgaa catatacagc tcgatgtcgt
    49321 tcgggttacg accaagcttt gtgaaagcat cctgcagata gtcaatttca tcttccgcca
    49381 gagccaggcc aagacgcagg ttagcgtcga tcagcgcctg acggccctgc cccagcaaat
    49441 caacgctggt aaccggagtc ggttgatggt gggcaaacaa ctgctctgca tcatctaaag
    49501 caaaaaagac cgtttccatc atgcggtcgt gcagttcagc ggtaacctgc tgccattgtt
    49561 cattggtcag cgtaccggct tctatatagt aagcaacgcc gcgctcaagg cggtttacct
    49621 gttgtagccc gcagttatga gcaatatcgg tcgctttcga agaccaggga gagatggtgc
    49681 caggacgcgg ggtcaccagc aggagtttgc cttgcggggc gtggctggcg agtgccgggc
    49741 catatttcag caggcgttca agttgtgcgt gctcatcatc gtttaacggc gcattgaggt
    49801 cagcaaaatg gacatactcg gcgtaaatat tgtgaaccgg gagcctggca gcctgaaaac
    49861 gtgccagcag tttgttgatt cggaatgccg acagtgcagg cgaaccacgc agaatttcca
    49921 tcataagtct ctcgtcttct aagctttcgg tgtacccaag gggggaaacg ggcgtcatta
    49981 taaagaatct gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt
    50041 agcaacgcgt gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat
    50101 gccgctcatt tatgagtaaa cctttcacta ttattacatt ttttcaagct gggacgcgca
    50161 cgacacagag aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc
    50221 tggcactgct gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca
    50281 accgtatcgc cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc
    50341 tgacgtataa cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt
    50401 ttgccgatta cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt
    50461 ttgacgacct cgataatggt aacgccgacc tgctggcggc aggacttgtc tataacagtg
    50521 agcgggtaaa aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt
    50581 ataaagtggg tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg
    50641 ttgcaccggg tcatgtggtg gttaacgatc tccagaccct gaaagaaaca aaattcccgg
    50701 aattaagctg gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg
    50761 aaggaaaact cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc
    50821 acccggagct cgccgtagcg ctcgatatca ccgatgaaca accggtgacc tggtttagcc
    50881 cgttagatgg cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg
    50941 aagacggtac gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt
    51001 acgtcgatac gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc
    51061 tgtttgagaa atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg
    51121 aatcgcactg ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa
    51181 ccaaaaatac cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca
    51241 gcggtggcgt gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga
    51301 acgagcggat ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgcaggatg
    51361 cccgcgccct gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc
    51421 gtctgccttt acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg
    51481 ggcatgaagc ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt
    51541 atctgcaaga gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc
    51601 cggcggtatc gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc
    51661 agtcgtcatc aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg
    51721 aagagaaaca aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc
    51781 ggcgcatgcg aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg
    51841 taatttccac tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag
    51901 cgccagtttt cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg
    51961 ctccggcaca cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat
    52021 aattttgcat caccagaccg ccctgcctca gggccatgat ttctgcatgt gcggtgggat
    52081 catgtcgacc aatcggacgg ttccagcctt cgccaataac acgattgtta tgcactaata
    52141 ccgcgccgac cggtacttcc cgctcatccc aggcacgctt cgccagcgtc attgcatgac
    52201 gcatccagta ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag
    52261 cgcggcgcat tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc
    52321 gcggggttac tcgccagcga tgctggcaaa agtaaagcag cgggttgtcc tgattactgt
    52381 cgctatagcc actgtacagc cgcagcggcg tgccgatttt gcgctccagt tgcgcgacct
    52441 tttcatgccc gaggcaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc
    52501 tggcgataag attcacccgc ggcagccagg gtgtatcgaa atagaccgct tcaaccagcg
    52561 gctgtggaga accggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta
    52621 atcgctcctg aaccagcgga aaggcggtaa cattgtcacg aaaccagcgt acgaaatcgg
    52681 cctgcaacgc ctgtaaacgt gcttcgctgt gaccaaaggt gcatccccac agaagcagac
    52741 tcatcggcca gcgtgccgca cgacctttta tcaataacgc aatggctata atcggcaaca
    52801 acggcaggac aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc
    52861 cgaacatatc ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt
    52921 gagttgccaa accatactcc tctggactga aacatcctga ttaattttcg ctactacata
    52981 gcctaacaga tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa
    53041 tttttaattc atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtgtctcata
    53101 ttgcctgttg tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc
    53161 gtcagcgtta ccaggggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac
    53221 aacctgatac ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc
    53281 agtccagcgt cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt
    53341 tggcgttgag tgaagcgctg gcaagccagc cggaatcgcc ctcagtgccc attcataacc
    53401 aaatccgcgg tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaatactg
    53461 ccgctatgta cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa
    53521 tgttgcgctc tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc
    53581 aaaactttgc ctggaagctg atgaagattg gcttcaatgc tgccgcagtg cgcgatatgc
    53641 atgcgctact cgctaccgta caggcgtcgt cccctgacga tctgttatta gccatttctt
    53701 acaccggtgt acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa
    53761 aagtgctggc gattaccggc tttactccca atgccctgca acagcgtgct tctcattgcc
    53821 tgtataccat tgccgaagaa caagcgacaa acagtgcttc aatctctgct tgtcacgcgc
    53881 agggaatgtt aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac
    53941 cagaacgtat tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc
    54001 gtataatgcc cgcccggttt gtgttgtttt gagagtttcc ttatggcgct gttaatcact
    54061 aaaaaatgca tcaattgtga tatgtgtgaa cccgaatgcc cgaatgaggc gatttcaatg
    54121 ggagatcata tctatgagat taacagcgat aagtgtaccg aatgcgtagg gcactacgag
    54181 acaccaacct gccagaaggt gtgcccgatc cccaatacta ttgtgaaaga tccgcaacat
    54241 gtcgagacag aagaacagtt gtgggataaa tttgtgctga tgcaccacgc ggataaaatt
    54301 taata
//
LOCUS       DAZFDC010000014        52764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFDC010000014 DAZFDC010000000
VERSION     DAZFDC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.014"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            252..1346
                     /gene="gcvT"
                     /locus_tag="ACHJ48_001003"
     CDS             252..1346
                     /gene="gcvT"
                     /locus_tag="ACHJ48_001003"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO4706611.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHEVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEGGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPKGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1370..1759
                     /gene="gcvH"
                     /locus_tag="ACHJ48_001004"
     CDS             1370..1759
                     /gene="gcvH"
                     /locus_tag="ACHJ48_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO4706612.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1877..4750
                     /gene="gcvP"
                     /locus_tag="ACHJ48_001005"
     CDS             1877..4750
                     /gene="gcvP"
                     /locus_tag="ACHJ48_001005"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO4706613.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDIFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4916..6349)
                     /gene="bglA"
                     /locus_tag="ACHJ48_001006"
     CDS             complement(4916..6349)
                     /gene="bglA"
                     /locus_tag="ACHJ48_001006"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO4706614.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINLEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDSDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            6394..6705
                     /gene="yqfB"
                     /locus_tag="ACHJ48_001007"
     CDS             6394..6705
                     /gene="yqfB"
                     /locus_tag="ACHJ48_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO4706615.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            6869..7528
                     /gene="yqfA"
                     /locus_tag="ACHJ48_001008"
     CDS             6869..7528
                     /gene="yqfA"
                     /locus_tag="ACHJ48_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO4706616.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7605..8585)
                     /gene="ygfZ"
                     /locus_tag="ACHJ48_001009"
     CDS             complement(7605..8585)
                     /gene="ygfZ"
                     /locus_tag="ACHJ48_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO4706617.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYLQGQ
                     VTADVSQMTDDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLAELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLCIEPLPYSLEE"
     gene            8828..9094
                     /gene="sdhE"
                     /locus_tag="ACHJ48_001010"
     CDS             8828..9094
                     /gene="sdhE"
                     /locus_tag="ACHJ48_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO4706618.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9075..9482
                     /gene="ygfX"
                     /locus_tag="ACHJ48_001011"
     CDS             9075..9482
                     /gene="ygfX"
                     /locus_tag="ACHJ48_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO4706619.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9522..10043)
                     /gene="fldB"
                     /locus_tag="ACHJ48_001012"
     CDS             complement(9522..10043)
                     /gene="fldB"
                     /locus_tag="ACHJ48_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO4706620.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10155..11051
                     /gene="xerD"
                     /locus_tag="ACHJ48_001013"
     CDS             10155..11051
                     /gene="xerD"
                     /locus_tag="ACHJ48_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO4706621.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11076..11786
                     /gene="dsbC"
                     /locus_tag="ACHJ48_001014"
     CDS             11076..11786
                     /gene="dsbC"
                     /locus_tag="ACHJ48_001014"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO4706622.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            11792..13525
                     /gene="recJ"
                     /locus_tag="ACHJ48_001015"
     CDS             11792..13525
                     /gene="recJ"
                     /locus_tag="ACHJ48_001015"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO4706623.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13616..14714
                     /gene="prfB"
                     /locus_tag="ACHJ48_001016"
     CDS             join(13616..13690,13692..14714)
                     /gene="prfB"
                     /locus_tag="ACHJ48_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO4706624.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14724..16241
                     /gene="lysS"
                     /locus_tag="ACHJ48_001017"
     CDS             14724..16241
                     /gene="lysS"
                     /locus_tag="ACHJ48_001017"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4706625.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIVESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16284..16832)
                     /gene="idi"
                     /locus_tag="ACHJ48_001018"
     CDS             complement(16284..16832)
                     /gene="idi"
                     /locus_tag="ACHJ48_001018"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO4706626.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            16887..16958
                     /locus_tag="ACHJ48_001019"
     CDS             16887..16958
                     /locus_tag="ACHJ48_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706627.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(16955..17080)
                     /gene="yqfG"
                     /locus_tag="ACHJ48_001020"
     CDS             complement(16955..17080)
                     /gene="yqfG"
                     /locus_tag="ACHJ48_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO4706628.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17082..18530)
                     /gene="uacT"
                     /locus_tag="ACHJ48_001021"
     CDS             complement(17082..18530)
                     /gene="uacT"
                     /locus_tag="ACHJ48_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO4706629.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAQLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18966..20885
                     /gene="ygfT"
                     /locus_tag="ACHJ48_001022"
     CDS             18966..20885
                     /gene="ygfT"
                     /locus_tag="ACHJ48_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO4706630.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20885..21373
                     /gene="ygfS"
                     /locus_tag="ACHJ48_001023"
                     /pseudo
     CDS             20885..21373
                     /gene="ygfS"
                     /locus_tag="ACHJ48_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(21409..22776)
                     /gene="ghxQ"
                     /locus_tag="ACHJ48_001024"
                     /gene_synonym="ygfQ"
     CDS             complement(21409..22776)
                     /gene="ghxQ"
                     /locus_tag="ACHJ48_001024"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO4706631.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22812..24128)
                     /gene="guaD"
                     /locus_tag="ACHJ48_001025"
     CDS             complement(22812..24128)
                     /gene="guaD"
                     /locus_tag="ACHJ48_001025"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO4706632.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLKEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24146..25546)
                     /gene="xanQ"
                     /locus_tag="ACHJ48_001026"
     CDS             complement(24146..25546)
                     /gene="xanQ"
                     /locus_tag="ACHJ48_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO4706633.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25711..28581)
                     /gene="xdhD"
                     /locus_tag="ACHJ48_001027"
     CDS             complement(25711..28581)
                     /gene="xdhD"
                     /locus_tag="ACHJ48_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO4706634.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVDMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28578..29357)
                     /gene="ygfM"
                     /locus_tag="ACHJ48_001028"
     CDS             complement(28578..29357)
                     /gene="ygfM"
                     /locus_tag="ACHJ48_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO4706635.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29407..30735)
                     /gene="ssnA"
                     /locus_tag="ACHJ48_001029"
     CDS             complement(29407..30735)
                     /gene="ssnA"
                     /locus_tag="ACHJ48_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO4706636.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPSPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30738..33836)
                     /gene="ygfK"
                     /locus_tag="ACHJ48_001030"
     CDS             complement(30738..33836)
                     /gene="ygfK"
                     /locus_tag="ACHJ48_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO4706637.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQDFHYVLIATGTDKNSGVKLAGDNQNVLKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34158..34736)
                     /gene="mocA"
                     /locus_tag="ACHJ48_001031"
     CDS             complement(34158..34736)
                     /gene="mocA"
                     /locus_tag="ACHJ48_001031"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO4706638.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            34839..35609
                     /gene="yqeC"
                     /locus_tag="ACHJ48_001032"
     CDS             34839..35609
                     /gene="yqeC"
                     /locus_tag="ACHJ48_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO4706639.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANLGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            35657..37282
                     /gene="yqeB"
                     /locus_tag="ACHJ48_001033"
     CDS             35657..37282
                     /gene="yqeB"
                     /locus_tag="ACHJ48_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO4706640.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIIDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFA
                     AGKDCHAVIETNRGHWLGQVIYCGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(37323..38255)
                     /gene="arcC"
                     /locus_tag="ACHJ48_001034"
     CDS             complement(37323..38255)
                     /gene="arcC"
                     /locus_tag="ACHJ48_001034"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4706641.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVVTLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(38303..39688)
                     /gene="hyuA"
                     /locus_tag="ACHJ48_001035"
     CDS             complement(38303..39688)
                     /gene="hyuA"
                     /locus_tag="ACHJ48_001035"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO4706642.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPPKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(39741..40952)
                     /gene="ygeY"
                     /locus_tag="ACHJ48_001036"
     CDS             complement(39741..40952)
                     /gene="ygeY"
                     /locus_tag="ACHJ48_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO4706643.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(41010..42206)
                     /gene="dpaL"
                     /locus_tag="ACHJ48_001037"
     CDS             complement(41010..42206)
                     /gene="dpaL"
                     /locus_tag="ACHJ48_001037"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO4706644.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(42264..43451)
                     /gene="ygeW"
                     /locus_tag="ACHJ48_001038"
     CDS             complement(42264..43451)
                     /gene="ygeW"
                     /locus_tag="ACHJ48_001038"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO4706645.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTSFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            43930..45708
                     /gene="ygeV"
                     /locus_tag="ACHJ48_001039"
     CDS             43930..45708
                     /gene="ygeV"
                     /locus_tag="ACHJ48_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO4706646.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLYRVAG
                     TGAYGKFLGRQLSGNSRLLCHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(45748..46227)
                     /gene="xdhC"
                     /locus_tag="ACHJ48_001040"
     CDS             complement(45748..46227)
                     /gene="xdhC"
                     /locus_tag="ACHJ48_001040"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO4706647.1"
                     /translation="MNHSETITIECTINGMPFQLHAVPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(46224..47102)
                     /gene="xdhB"
                     /locus_tag="ACHJ48_001041"
     CDS             complement(46224..47102)
                     /gene="xdhB"
                     /locus_tag="ACHJ48_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO4706648.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDSITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(47113..49410)
                     /gene="xdhA"
                     /locus_tag="ACHJ48_001042"
     CDS             complement(47113..49410)
                     /gene="xdhA"
                     /locus_tag="ACHJ48_001042"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO4706649.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWEELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKTFVGGSFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYVSHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNASREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            49826..50581
                     /gene="actS"
                     /locus_tag="ACHJ48_001043"
     CDS             49826..50581
                     /gene="actS"
                     /locus_tag="ACHJ48_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO4706650.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            50660..50733
                     /locus_tag="ACHJ48_001044"
     tRNA            50660..50733
                     /locus_tag="ACHJ48_001044"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50692..50694,aa:Gly,seq:ccc)
     gene            50967..52572
                     /locus_tag="ACHJ48_001045"
                     /pseudo
     CDS             50967..52572
                     /locus_tag="ACHJ48_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
     gene            52639..>52764
                     /locus_tag="ACHJ48_001046"
     CDS             52639..>52764
                     /locus_tag="ACHJ48_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFO4706651.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTW"
BASE COUNT        13031 a        13049 c        13708 g        12976 t
ORIGIN      
        1 ctcatcacat catctttata tagaaacttg tgtatttttt ggttttttat tctgttgcga
       61 ttttggcgtt ttttaaccat aagctaatgt gatgatcaat tttaccttat ggttaacagt
      121 ctgtttcggt ggtaagttca ggcaaaagag aacgattgcg ttggggaccg ggagtggctc
      181 cgatgctggg tttcgtggtg ataatttcac catgaaaaag ttgtcagccc cgcttattca
      241 atgaggacaa gatggcacaa cagactcctt tgtacgaaca acacacgctt tgcggcgctc
      301 gcatggtgga tttccacggc tggatgatgc cgctgcatta cggttcgcaa atcgacgaac
      361 atcatgaggt acgtaccgat gccggaatgt ttgatgtgtc acatatgacc atcgtcgatc
      421 ttcgcggcag ccgcacccgg gagtttctgc gttatctgct ggcgaacgat gtggcgaagc
      481 tcaccaaaag cggcaaagcc ctttactcgg ggatgttgaa tgcctctggc ggtgtgatag
      541 atgacctcat cgtctactac tttactgaag atttcttccg cctcgttgtt aactccgcca
      601 cccgcgaaaa agacctctcc tggattaccc aacacgctga acctttcggc atcgaaatta
      661 ccgttcgtga tgacctttcc atgatcgccg tacaagggcc gaatgcgcag gcaaaagctg
      721 ccacactgtt taatgatgct cagcgtcagg cggtggaagg gatgaaaccg ttctttggcg
      781 tgcaggcggg cgatctgttt attgccacca ctggttatac cggtgaagcg ggctatgaaa
      841 ttgcgctgcc caatgaaaaa gcggccgatt tctggcgtgc gctggtggaa gggggcgtta
      901 agccatgcgg tctgggcgcg cgtgacacgc tgcgtctgga agcgggcatg aatctttatg
      961 gtcaggagat ggacgaaacc atctctccgt tagccgccaa catgggctgg actatcgcct
     1021 gggaaccggc agatcgtgac ttcatcggtc gtgaagccct ggaagtgcag cgtgagcatg
     1081 gcacagaaaa actggttggt ctggtgatga ccgaaaaagg cgtgctgcgt aatgaactgc
     1141 cggtacgttt taccgatgcg cagggcaacc agcatgaagg cattatcacc agcgggactt
     1201 tctccccgac gctgggttac agcattgcgc tggcacgcgt gccgaaaggt attggcgaaa
     1261 cggcgattgt gcaaattcgc aaccgtgaaa tgccggttaa agtgacaaaa cctgtttttg
     1321 tgcgtaacgg caaagccgtc gcgtgattta cttttttgga gattgattga tgagcaacgt
     1381 accagcagaa ctgaaataca gcaaagaaca cgaatggctg cgtaaagaag ccgacggcac
     1441 ttacaccgtt ggtattaccg aacatgctca ggagctgtta ggcgatatgg tgtttgttga
     1501 cctgccggaa gtgggcgcaa cggttagcgc gggcgatgac tgcgcggttg ccgaatcagt
     1561 aaaagcggcg tcagacattt atgcgccagt aagcggtgaa atcgtggcgg taaacgacgc
     1621 actgagcgac tccccggaac tggtgaacag cgaaccgtat gcaggtggct ggatctttaa
     1681 aatcaaagcc agcgatgaaa gcgaactgga atcactgctg gatgcgaccg catacgaagc
     1741 attgttagaa gacgagtaac ggctttattc ctcttctgcg ggagaggatc agggtgagga
     1801 aatttacacc tcaccctcac tctcttcgca aggagagagg ttcacaattc actgcacgtt
     1861 tcaggaacca tcgctcatga cacagacgtt aagccagctt gaaaatagcg gcgcttttat
     1921 tgaacgccat atcggaccgg acgccgcgca acagcaagaa atgctgaatg ccgttggcgc
     1981 acaatcgtta aacgcgctga ccggccagat tgtaccgaaa gatattcagc ttgcgacccc
     2041 gccgcaggtt ggcgcaccgg cgaccgaata cgccgcgctg gcggaactca aggctattgc
     2101 cagtcgcaat aaacgcttca cgtcttacat cggcatgggt tacaccgccg tgcagttacc
     2161 gccggttatc ctgcgtaaca tgctggaaaa tccgggctgg tataccgcgt atactccgta
     2221 tcaacctgaa gtctcgcagg gccgccttga ggcactgctc aatttccagc aggtaacgct
     2281 ggatttaacc gggctggata tggcctctgc ttctcttctt gacgaagcca ccgctgccgc
     2341 cgaagcaatg gcgatggcga aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt
     2401 ggcttccgat gtgcatccgc aaacgctgga tgtggtccgt actcgtgccg aaacctttgg
     2461 ttttgaagtg attgtcgatg atgcgcaaaa agtgctcgac catcaggaca tcttcggcgt
     2521 gctgttacag caggttggca ctaccggtga aattcacgac tacactgcgc ttatcagcga
     2581 actgaaatca cgcaaaattg tggtcagcgt tgccgctgat attatggcgc tggtgctgtt
     2641 aactgcgccg ggtaaacagg gcgcggatat tgtttttggt tcggcgcaac gctttggcgt
     2701 accgatgggt tacggtggcc cacacgcggc attctttgcg gcgaaagacg aatacaaacg
     2761 ctcaatgcca ggccgtatta tcggtgtatc gaaagatgca gctggcaata ccgcgctgcg
     2821 catggcgatg cagactcgcg agcaacatat tcgtcgtgag aaagcgaact ccaacatttg
     2881 tacttctcag gtgctgctgg caaacatcgc cagcctgtat gccgtttatc acggcccgat
     2941 tggcctgaaa cgtatcgcta accgcattca ccgtctgacc gatatcctgg cggcaggcct
     3001 gcaacaaaaa ggtctgaagc tgcgccatgc gcactatttc gacactctgt gtgtggaagt
     3061 ggccgacaag gcgggcgtac tggcgcgtgc cgaagcggct gaaatcaacc tgcgtagcga
     3121 tatcctgaac gcggttggga tcacccttga tgaaaccacc acgcgcgaaa acgtgatgca
     3181 gcttttcagc gtactactgg gggataacca cgggctggac atcgacacgc tggacaaaga
     3241 cgtggctcac gacagccgct ctatccagcc tgcgatgctg cgcgacgacg aaatcctcac
     3301 ccatccggtg tttaatcgct accacagcga aaccgaaatg atgcgctata tgcactcgct
     3361 ggagcgtaaa gatctggcgc tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa
     3421 actgaacgcc gccgccgaga tgatcccaat cacctggccg gaatttgccg aactgcaccc
     3481 gttctgcccg ccggagcagg ccgaaggtta tcagcagatg atcgcgcagc tggctgactg
     3541 gctggtgaaa ctgaccggtt acgacgccgt ttgtatgcag ccgaactctg gcgcacaggg
     3601 cgaatacgcg ggcctgctgg cgattcgtca ttatcatgaa agccgcaacg aagggcatcg
     3661 cgatatctgc ctgatcccgg cttctgcgca cggcactaac cccgcttctg cacatatggc
     3721 aggaatgcag gtggtggttg tggcgtgtga taaaaacgga aatatcgatc tgactgatct
     3781 gcgcgcgaaa gccgaacagg caggtgataa tctctcctgc atcatggtga cctatccttc
     3841 cacccacggt gtgtatgaag aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg
     3901 cggtcaggtt taccttgatg gcgcgaacat gaacgcccag gttggcatca cctcgccggg
     3961 ctttattggc gcagacgttt cacaccttaa cctgcataaa actttctgca ttccgcacgg
     4021 cggtggtggt ccgggtatgg gaccgatcgg cgtgaaagcg catctggctc cgtttgtacc
     4081 gggtcatagc gtggtgcaaa tcgaaggcat gttaacccgt cagggcgcgg tttctgcggc
     4141 accgttcggt agcgcctcta tcctgccaat cagctggatg tacatccgca tgatgggcgc
     4201 agaagggctg aaaaaagcaa gccaggtggc aatcctcaac gccaactata ttgccagccg
     4261 cctgcaggat gccttcccgg tgctgtatac cggtcgtgac ggtcgcgtgg cgcacgaatg
     4321 tattctcgat attcgtccgt tgaaagaaga aaccggcatc agcgagctgg atattgccaa
     4381 gcgcctgatc gactacggtt tccacgcgcc gacgatgtcg ttcccggtgg caggtacgct
     4441 gatggttgaa ccgactgaat ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat
     4501 gctggctatc cgcgcagaaa ttgaccaggt gaaagccggt gtctggccgc tggaagataa
     4561 cccgctggtg aacgcgccgc acattcagag tgaactggtc gccgagtggg cgcatccgta
     4621 cagccgtgaa gttgcggtat tcccggcagg cgtggcagac aaatactggc cgacagtgaa
     4681 acgtttggat gatgtttacg gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga
     4741 ataccagtaa ttcactgatt cgactatctt ctaaaggcgc ttcggcgcct ttttagattg
     4801 ttaataaagt gcgctttgtg tgtttcggct gaggcgtaat ataaaatatt gcgagctcaa
     4861 tatattaaaa gagtgccagg cctgatgagc gtagcgcatc aggcacatcg gctaattaca
     4921 gattctcgcc gttgctggca atcacttctt tgtaccagtt aaagctcttc ttacgtgaac
     4981 gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg tagcgtttgc
     5041 tgtactgccc ggtagtgaac gacacgcagt cgatgcagcc ccacggtgtg tagcccatca
     5101 gatccacgcc atcgtaagtt actgcttttt tcatctcttc aatatgggcg cgcaggtagt
     5161 caatgcggta gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa gcgccaaaac
     5221 cgttttcgac aataaacagc ggcttctggt aacgctcata cagttcgcaa agtgcgtaac
     5281 gcagacccac cggatcaatc tgccagcccc agtcagatgc tttaacatac gggtttggta
     5341 cgctgccttc aaaaccagag atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg
     5401 tcatgtaata gctgaaacca agataatcgc aggtgccttc gcgcagtaca tcgagatcgc
     5461 tatcttccat tttgatgtta aatccgcgac gctcccactc gttcaacaca tacgacgggt
     5521 aatagccgcg tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga
     5581 acatcacatc gtccgggtta catgagtagg gatagagcgg caccatcgcc agcatacagc
     5641 cgactttcat ctccaggtta atgcgacgcg cggctttcac tgccagggcg ctggcgacaa
     5701 actggtgatg cagcacctga tacatcgtct cttccgggtt ttcatgctcg gtatacacca
     5761 cgccggagca gcagtaaccg aacagcggtg cacgccagtt acgctggttg ttaatttcgt
     5821 tgaaggtcat ccagtatttg actttgtgct tatagcgttc aaataccact tccgcgaaac
     5881 gtacaaagaa atcaaccact ttacggttgg tccagctacc gtattgctgc accagatgca
     5941 gcggcatttc aaagtgggag agggtgatca ccggttcgat gttgtatttc agcagttcat
     6001 cgaacagaga gtcgtagaac ttcagccctt cttcgtttgg ctgagcttca tcgccttttg
     6061 gaaaaatgcg cgtccaggca atggatgtac gaaaacattt gaagcccatt tcggcaaata
     6121 gcttgatgtc ttccttatag tgaccataaa aatcaacggc ttcatggttt ggatagtatt
     6181 tgcctggcaa gacttctttg gttatttcgc gcggcacgcc gtgtgcgcca ccggtcagaa
     6241 cgtcacaaat gctcggccct tttccgcctt tgttccagcc gccttcgacc tgatgagcgg
     6301 caactgcgcc gccccataag aaatctttcg gtaaggtgag ttttttcact atcataggct
     6361 ccagatgagt caattcttac aggaaatagt accatgcagc caaacgacat cacttttttt
     6421 caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg cgacgagtct
     6481 gaatcgcact tcaaaacggg tgatgtgctt cgtgtcggac gttttgaaga tgacggttat
     6541 ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacgct gacggaaaaa
     6601 catgcagagc aggaaaatat gaccctgact gaactgaaaa aggtcattgc cgacatctat
     6661 cccggccaga cacagtttta tgtgattgaa tttaaatgcc tttaaattga actcacgcct
     6721 gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc aaatcagaat
     6781 ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag aggtatacat
     6841 gtgcgtgtac gtttctccgg agtaagttat ggttcagaag cccctcatta agcagggata
     6901 ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggttgg tgtttggtat
     6961 cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg ccacagcgat
     7021 aaccagctac agcctctatg gcggcagtat gatcctgctg ttcctcgctt cgacgctcta
     7081 tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc attgcgctat
     7141 ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg attctccgtt
     7201 agcgcgcggg ttgatgattg ttatctggag cctggcattg ctggggattc tgttcaaact
     7261 gaccatcgcg caccgattca aaattttatc tctggtgact tatctggcga tgggctggct
     7321 gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg ttaccttact
     7381 ggcggtaggc ggcgtggttt attcgctcgg ggtgattttc tacgtctgca aacgcattcc
     7441 atacaaccat gccatctggc acggcttcgt gctcggcggt agtgtgtgcc actttctggc
     7501 gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg
     7561 ccagcgtcgc atcaggcgat aaatgcctga tgcgacacag agatttactc ttcgagcgaa
     7621 tacggcagtg gctcgataca caatgtattc gcatcgtcgc gcacgcggaa gatgctgtcc
     7681 ggttccatat cgttattcat cactacctgc accacgacct gaccatcttc cagttttacc
     7741 gcagccagca ccgtaccagt acgacgccag ttctcgccca ttttcagctc taagtcttca
     7801 ccagcttccg gcagtcggct ggcactacct gtcagcaacc agagcgcgcg tttgttggca
     7861 ccacggaatt ttgctcgcgc caccatctct tgtccggtat agcagccttt cttaaagctg
     7921 ataccgccca gcgcctggag gttggtcgcc tgtgggataa actgcccgct gttcgcggca
     7981 tcaatcaccg ggaaacccgc ttcaatgttt aatgccagcc actgttggct attgttcagt
     8041 tccgcttcac cgcgcagttt atcggtcagc atattggcag tagcttcatc ggttacgatc
     8101 aggaaacgtt ctgccgggtg ttcaaaccat agcaaagtgg tcgcgccttc tttaactacc
     8161 tgtttttctt tcgaaggcag ttcactaaag atatttgcca gcgcggcgcg cgcctgaaaa
     8221 ccggcaacac caagcagcac acgctcgtcg tctggcgcga tggtcacttt agagaacacc
     8281 gcatattttt tcagttcagc cagctgcggt tcacgcacgc tgcgccgttc aatccatgca
     8341 aagccatcgc cgtcgcggaa cagacgtaaa ttgctccaca ttttaccttt ggcgtcgcaa
     8401 tgggcggcga gcaggtgctg atcatctgtc atctggctga catctgctgt cacctggccc
     8461 tgcaaatatt tttcgctgtc cgcgccagta atggtggcaa gcgcccagtc atcaagcgtc
     8521 atcagcgtca gtggcaaacg ggcagaagcc gtaggctgac ggggaggaaa aggtgtaaaa
     8581 gccataatag agtcctgagt tgcttaacgg cgtattaata actaatggta aaagagctat
     8641 gaggcaatgc aagtgctttc actacgcgat ctgtgccttc tgcgaggcga cttcccaaaa
     8701 aatgaagccc ttaaattgtt gtgaattaat ttcgcgatcg cgattgtgtt tactgatatt
     8761 cccgtaaaag aagtcgaaaa acacgttaca atagccgggt actggatttt cgtgagacag
     8821 gaagaagatg gacattaaca acaaagcccg cattcattgg gcatgccgcc gtggtatgcg
     8881 cgaactcgat atttcaatca tgccgttttt cgaacatgag tatgacagct taagcgatga
     8941 cgaaaaacgc atctttattc gtctgctgga atgtgacgat cccgatctgt ttaactggct
     9001 gatgaatcac ggtaaaccgg ccgatgcaga actggaaatg atggtccgac tcatccagac
     9061 acggaaccgg gaacgtggtc ctgtggcaat ctgatttgcg cgtctcctgg cgcgcacagt
     9121 ggctttcctt gctgattcat gggctggttg ccgctgttat tttactcatg ccctggccgc
     9181 tcagttacac cccgttatgg atggtgttac tttcgctggt ggtgtttgat tgcgttcgca
     9241 gccagcggcg tattaatgct cgccaggggg aaattcgctt gttgatggac gggcgtttgc
     9301 gttggcaagg gcaggagtgg agcatcgtca aagcgccgtg gatgattaag agcggcatga
     9361 tgctgcgttt acgttctgat agcggtaaac ggcaacattt atggctggca gccgacagca
     9421 tggacgaagc cgaatggcgg gatttacggc ggattttgtt gcaacaagag acgcaaagat
     9481 aaagatgacg gcaaagcata tgcgctaagc cgtcagtgat atcaggcgta atgctctgcc
     9541 atttcgttga ggatttgctc gcaccagctc tgaatacgct cgtcgctaag gtcatactgg
     9601 ttagtttcgt ccagcgccag ccccacgaac agttgcccgt cagcaatcac cggtttcggg
     9661 ctggtaaatt catacccttc cgttggccag tagccgacga acttcacgcc tttggtcgag
     9721 agtttgtcat gcagcatacc gagcgcatcg aggaaccact cgccgtatcc cagttgatcg
     9781 ccaagcccat acagcgcaac aattttacct tcgaggttca ggtcgtcgag ctgatcccag
     9841 acggcttccc agtcttcctg gatttcgcca aaatcccagg tcgggatacc cagaatgagc
     9901 acatcgtact gctccattaa tttcggggag tcgtccttga ggttatgtaa ggtcaccagt
     9961 tctgggccga taatatcgcg gattttttct gccgccattt cggtgtaaca ggtgctggaa
    10021 ccgtaaaaaa gacccatatt cataacgtaa gcttctcgat tctgctgtgg cttttgcggc
    10081 tagtgtatca gaatcgcttt agtcacggca caataaggca taatggatca aatgagtgac
    10141 cataaggggc gcaagtgaaa caggatctgg cacgcatcga gcagtttctt gatgctctgt
    10201 ggctggaaaa aaatctggct gaaaatacgt tgaacgctta ccgtcgcgat ctgtcaatga
    10261 tggtggagtg gttgcatcac cgcgggttga cgctggcgac ggcgcaaagt gacgatttac
    10321 aggcattact ggcagaacgg ctggagggcg ggtataaagc caccagctca gcgcgtttgc
    10381 tgagtgcagt gcgccgattg ttccagtatc tttatcgcga aaagtttcgt gaagatgatc
    10441 ccagtgcgca tctcgcttca ccgaaattgc cccagcgttt gccaaaagat ttaagcgaag
    10501 cgcaggtcga acgtttatta caggcaccat taattgatca gccactggag ctacgcgata
    10561 aagctatgct tgaagtgttg tatgctaccg ggctgcgtgt ctctgaactg gtcgggctga
    10621 caatgagtga tatcagcctg cgtcagggcg tagtgcgggt cattggtaaa ggcaacaaag
    10681 agcgcctggt gccgttaggt gaagaggcgg tttactggct ggaaacctat ctggaacatg
    10741 ggcgtccgtg gctactgaat ggtgtgtcaa ttgacgtgtt gtttcccagc cagcgtgcgc
    10801 agcagatgac gcgacagacc ttctggcacc gcattaaaca ttatgccgtg ctggcgggta
    10861 tcgacagcga aaagctgtca ccgcatgtgt tgcgtcacgc ttttgccact cacttattaa
    10921 atcatggtgc ggatttacgc gtggtgcaga tgctactggg ccacagcgat ctctccacca
    10981 cgcaaattta tacgcatgtc gctaccgagc gtctgcggca acttcatcaa cagcatcacc
    11041 cgcgggcgtg atgtctgaaa agaacgggaa gatttatgaa gaaaggtttt atgttgttta
    11101 ctttgttagc ggcgttttca ggcttcgctc aggctgatga cgcggcaatt caacaaacat
    11161 tagccaaaat gggcatcaaa agcagcgata ttcagcccgc gcccgtagct ggcatgaaga
    11221 ctgtgctgac taacagcggt gtgttgtaca tcaccgatga tggtaaacat atcattcagg
    11281 ggccaatgta tgacgttagc ggcacggctc cggtcaatgt caccaataag atgctgttaa
    11341 agcagttgaa tgcgcttgaa aaagagatga tcgtttataa agcaccgcag gaaaaacacg
    11401 tcatcaccgt gtttactgat attacctgtg gttactgcca caaactgcat gaacaaatgg
    11461 cagactataa cgcgctgggg atcaccgtgc gttatcttgc tttcccacgc caggggctgg
    11521 acagcgatgc tgagaaagaa atgaaagcta tctggtgtgc gaaagataaa aacaaagcgt
    11581 ttgatgatgt gatggcaggt aaaagcgtcg caccagccag ctgcgacgtg gatattgccg
    11641 accattacgc acttggcgtc cagcttggcg ttagcggtac tccggcagtt gtgctgagca
    11701 atggcacact tgttccgggt taccagccgc cgaaagagat gaaagaattc ctcgacgaac
    11761 accaaaaaat gaccagcggt aaataattcg cgtgaaacaa cagatacaac ttcgtcgccg
    11821 tgaagtcgat gaaacggcag acttgcccgc ggaattgcct cccttgctgc gccgtttata
    11881 cgccagccgg ggagtacgca gtgcgcaaga actggaacgc agtgttaaag gtatgctgcc
    11941 ctggcagcaa ctgagcggcg tcgaaaaggc cgttgagatc ctttacaacg cttttcgcga
    12001 aggaacgcgg attattgtgg tcggtgattt cgacgccgac ggtgccacca gcacggctct
    12061 aagcgtgctg gcgatgcgct cgcttggttg cagcaatatc gactacctgg taccaaaccg
    12121 tttcgaagac ggttacggct taagcccgga agtggtcgat caggcccatg cccgtggcgc
    12181 gcagttaatt gtcacggtgg ataacggtat ttcctcccat gcgggagttg agcacgctcg
    12241 ctcgttgggc atcccggtta ttgttaccga tcaccatttg ccgggcgaca cattacccgc
    12301 agcggaagcg atcattaacc ctaacttgcg cgactgtaat ttcccgtcga aatcactggc
    12361 aggcgtgggt gtggcgtttt atctgatgct ggcgctgcgc acctttttgc gcgatcaggg
    12421 ctggtttgat gagcgcggca tcgcaattcc taacctggca gaactgctgg atctggtcgc
    12481 gctggggaca gtggcggacg tcgtgccgct ggacgctaat aatcgcattc tgacctggca
    12541 ggggatgagt cgcatccgtg ccggaaagtg ccgtccaggg attaaagcgc tgctggaagt
    12601 ggcaaaccgt gatgcacaaa aactcgccgc cagcgattta ggttttgcgc tggggccacg
    12661 tctcaatgct gccgggcgac tggacgatat gtccgtcggt gtggcgctgt tgctgtgcga
    12721 caacatcggc gaagcgcgcg tgctggcaaa tgaactcgat gcgctaaatc agacgcgaaa
    12781 agagatcgaa caaggaatgc agattgaagc cttgaccctg tgcgagaaac tggagcgaag
    12841 tcgcgacacg ctacccggcg ggctggcaat gtatcacccc gaatggcatc agggcgttgt
    12901 cggtattctg gcttcgcgca tcaaagagcg ttttcaccgt ccggttatcg cctttgcgcc
    12961 agcaggtgat ggtacgctga aaggttccgg tcgctccatt caggggctgc atatgcgtga
    13021 tgcgctggag cgattagaca cactctaccc cggtatgatg ctgaagtttg gcggtcatgc
    13081 gatggcggcg ggtttgtcgc tggaagagga taaattcgaa ctctttcaac aacggtttgg
    13141 cgagttggtt accgagtggc tggacccttc gctattgcaa ggcgaagtgg tgtcagacgg
    13201 cccgttaagc ccggccgaaa tgaccatgga agtggcgcag ctgctgcgcg atgctggccc
    13261 gtgggggcag atgttcccgg agccgctgtt tgatggtcat ttccgtctgc tgcaacagcg
    13321 gctggtgggc gaacgtcatt tgaaagtcat ggtcgaaccg gtcggcggcg gtccgctgct
    13381 ggatggtatt gcttttaatg tcgataccgc cctctggccg gataacggcg tgcgcgaagt
    13441 gcaactggct tacaagctcg atatcaacga gtttcgcggc aaccgcagcc tgcaaattat
    13501 catcgacaat atctggccaa tttagcgtca tcttctctat aaaaaagagc gtggattggg
    13561 tacaatcccg ctcttatcac cgcattttga ctagctcaat aaaagaaatc agaccatgtt
    13621 tgaaattaat ccggtaaata atcgcattca ggacctcacg gaacgctccg acgttcttag
    13681 ggggtatctt tgactacgac gccaagaaag agcgtctgga agaagtaaac gccgagctgg
    13741 aacagccgga tgtctggaac gaacccgaac gcgcacaggc gctgggtaaa gagcgttcct
    13801 ccctcgaagc cgttgtcgac accctcgacc aaatgaaaca ggggctggaa gatgtttctg
    13861 gtctgctgga actggctgta gaagctgacg acgaagaaac ctttaacgaa gccgttgctg
    13921 aactcgacgc cctggaagaa aaactggcgc agcttgagtt ccgccgtatg ttctctggcg
    13981 aatatgacag cgccgactgc tacctcgata ttcaggcggg gtctggcggt acggaagcac
    14041 aggactgggc gagcatgctt gagcgtatgt atttgcgctg ggcagaatcg cgtggtttca
    14101 aaactgaaat catcgaagag tcggaaggtg aagtggcggg tattaaatcc gtgacgatca
    14161 aaatctccgg cgattacgct tacggctggc tgcgtacaga aactggcgtt caccgcctgg
    14221 tgcgtaagag cccgtttgac tccggcggtc gtcgccacac gtcgttcagc tccgcgtttg
    14281 tttatccgga agttgatgat gatattgata tcgaaatcaa cccggcggat ctgcgcattg
    14341 acgtttatcg cgcgtccggc gcgggcggtc agcacgttaa ccgtaccgaa tctgcggtgc
    14401 gtattaccca catcccgacc gggatcgtga cccagtgcca gaacgaccgt tcccagcaca
    14461 agaacaaaga ccaggccatg aagcagatga aagcgaagct ttatgaactg gagatgcaga
    14521 agaaaaatgc cgagaaacag gcgatggaag ataacaaatc cgacatcggc tggggcagcc
    14581 agattcgttc ttatgtcctt gatgactccc gcattaaaga tctgcgcacc ggggttgaaa
    14641 cccgcaatac gcaggccgtg ctggacggca gcctggatca atttatcgaa gcaagtttga
    14701 aagcagggtt atgaggaacc aacatgtctg aacaacacgc acagggcgct gacgcggtag
    14761 tcgatcttaa caatgaactg aaaacgcgtc gtgagaagct ggcgaacctg cgtgagcagg
    14821 gaattgcctt cccgaacgat ttccgtcgcg atcatacctc tgaccaattg cacgcagaat
    14881 tcgacggtaa agagaacgaa gaacttgaag cgctgaacat cgaagtcgcc gttgctggcc
    14941 gcatgatgac ccgccgtatt atgggtaaag cctcttttgt taccctgcag gacgttggcg
    15001 gtcgtattca gctgtacgtt gcccgtgacg atcttccgga aggcgtttac aacgagcagt
    15061 tcaaaaaatg ggacctcggc gacatcctcg gcgcgaaagg taaactgttc aaaaccaaaa
    15121 ccggcgaact gtctatccac tgtaccgagt tgcgtctgct gaccaaagca ctgcgtccgc
    15181 tgccggataa attccacggc ttgcaggatc aggaagcgcg ctatcgtcag cgttatctcg
    15241 atctcatctc caacgatgaa tcccgcaaca cctttaaagt gcgctcgcag atcctctctg
    15301 gtattcgcca gttcatggtg aaccgcggct ttatggaagt tgaaacgccg atgatgcagg
    15361 tgatccctgg cggtgccgct gcgcgtccgt ttatcaccca ccataacgcg ctggatctcg
    15421 acatgtacct gcgtatcgcg ccggaactgt acctcaagcg tctggtggtt ggtggcttcg
    15481 agcgtgtatt cgaaatcaac cgtaacttcc gtaacgaagg tatttccgta cgtcataacc
    15541 cagagttcac catgatggaa ctctatatgg cttacgcaga ttacaaagat ctgatcgagc
    15601 tgaccgaatc gctgttccgt actctggcac aggatattct cggtaagacg gaagtgacct
    15661 acggcgacgt gacgctggat ttcggtaaac cgttcgaaaa actgaccatg cgtgaagcga
    15721 ttaagaaata tcgcccggaa accgacatgg cggatctgga caacttcgac tctgcaaaag
    15781 cgattgttga atctatcggt atccacgttg agaagagctg gggtctgggc cgtatcgtta
    15841 ccgagatctt cgaagaagtg gcagaagcac atctgattca gccgaccttc attactgaat
    15901 atccggcaga agtttctccg ctggcgcgtc gtaacgacgt taacccggaa atcacagacc
    15961 gctttgagtt cttcattggt ggtcgtgaaa tcggtaacgg ctttagcgag ctgaatgatg
    16021 cggaagatca ggcgcaacgc ttcctggatc aggttgccgc gaaagatgca ggtgacgacg
    16081 aagcgatgtt ctacgacgaa gattacgtca ccgcactgga acatggctta ccgccgacag
    16141 caggtctggg gattggtatc gaccgtatgg taatgctgtt caccaacagc cataccatcc
    16201 gcgacgttat tctgttcccg gcgatgcgtc cggtgaaata agcattacgt tatgcgcata
    16261 accccggcag atgtcggggt tttttaattg tgctgcgcga aagcagacaa caattttctt
    16321 gcttcactat tggctgcctg catcaccatc cacggactga acgcccacgg cgtggcatca
    16381 ataccgtgta atacatctgc taaatcacac cattgataat ccatcacttc atcatcgttg
    16441 atctgtaacg cactggtggt gcgtgcggca aataccggac acacttcatt ttccacaatg
    16501 ccattcggat cggtggcgcg atagcgaaag tcaggataga tagattcagg aggcgtaatt
    16561 tccacgccaa gctcataacg gcaacggcgg atcaccgcgt cttcgttgct ttctcccagt
    16621 tgtgggtgcc cacaaaccga gttagtccac acgccaggcc atgctttttt gctcagtgcg
    16681 cggcgggtaa ctaataattg tcctttggca ttaaacagcc aactggagaa cgcgagatgt
    16741 aagcgggtgt ctgccgtgtg tgcggcatac ttttccagcg tacccgtggg aactccctgt
    16801 gcattcaata aaatgacgtg ttccgtttgc ataatttctc acatgtaatt ctgatcactc
    16861 atcattatag ataattgaag agtgaaatga ttaaccaagt gagcgtttat cgacaaccgc
    16921 ccgttttgag cggatgccga caggtaaaaa ccatttaaaa aagtagcatc atatatttga
    16981 agcgactttc aatggccatt gcaacacagt cagaaatgac aggaatagag agagtaaaaa
    17041 gcaacagcag actgaatata gcgcgcatta aaaaattcat attattctcc atgctcattt
    17101 ttcttcagct tgcgcatcag aagccacata cgtactgtcc tgacttttaa atctttatca
    17161 gagacggatt ccttcaccag gtcagcatga tgctgatagc cattaaagaa gacgttcagc
    17221 acaacggcgc taagggttgc gagcataatg ccgctatgta gcagcggttg cagtacggcc
    17281 ggtaacttag aaaagaaatc gtgagagagc gttggagtca tgccaacgcc gagactgatc
    17341 gccacaatat agaggttgta acggttggtg gtgtagttac agcgcgacag aattcgaatc
    17401 cctgtcgcca gtaccatgcc gaacatcacc agaccagcgc cgcccagcac aaattgcgga
    17461 atggaggcta ccagcaccgc catttttggc accatgccaa acaggattaa tataattccc
    17521 gaagaaatac acacccagcg gctatgaacg cgcgtcacgc taaccaggcc aacgttttga
    17581 gaaaatgacg tgtgggggaa gctgttaaac gtgccaccaa tcattgtccc tacgccatcg
    17641 acacgcagcc cgcgaataat atcgtgcgaa gagagtttac gaccgactat ttcacccagt
    17701 gccaggaaca tccccatcga ctcgataaac acgatgatta acacggcagt catggtcaga
    17761 atggaaacgg gatcgaaaat cggcatacca aacgacatcg gggtgacaat cgcaaaccac
    17821 gaggcatcat gtagcccgga taaattgact tcattcatca tccacgaaag taaaaagcca
    17881 aatacaatcc ccagtaatac ggcgacgttg gacataaatc ctttcgcata gcgagtaatg
    17941 agcaagataa aaattaagac ggcaaaggag atacctaaat aaacgggatt accatattgc
    18001 ggattccctt tacctccggc ggcccagtca atacccacct gaatgatgct aagcccgata
    18061 gaagtaataa ccacaccggt aaccagtggc gggaataaag gcatcaagcg accgataagt
    18121 tgcgctaata atgtggtgat aaaacccgcg gcgatagtgg caccaaatat acccagcagg
    18181 ccgatatccg ggttcatccc aatggctatc attggtgtta cagcagcaaa ggtcaccgac
    18241 ataatcaccg gcaggcggat ccccataaag cggccgatac cgatacattg caataatgtg
    18301 acgatcccgc agcaaaagag atccgagcta atgagcatcg caatagcttc ttttgagagg
    18361 cccagtcggt caccaatcat aagaggaaca gcgactgcac ctgcgtacat gacaaggacg
    18421 tgttgcagac cgagtatgat cagctttcct ggtgataata tgcggtcaac ctcatcagtt
    18481 gggcggtctt gcccagaaga tgagggaagt tgggaatcta tggcgctcat cagtgtttct
    18541 cctttcgccc gccagagtaa aaagcaaccg tttacgttaa tgaaactgtc taaacacatg
    18601 ctcgtggagt gccagttttt gcgaggaagg ggagttagag acaggaattg cggaggcaat
    18661 cacataatcg tgcttaaact acgaaaatga caagagaacc tgacaatgga attatctgta
    18721 attttctcat attgatattt cattaatcat tctggcatgt cgcactctcg catttaatcg
    18781 tttttatctg gatagcgcta tttggggcgg caatgttgaa ataatgaaag cggcatagat
    18841 tgaggggcaa taatttgaac tcagtcgcaa aattataatt aaaacggtca tgctacctaa
    18901 tatatgcccg tttattgcat cgcgaaattt gagaagacat aactcattaa atgaaaggga
    18961 tgcaaatgaa taagtttatc gctgctgaag ccgcggaatg tataggctgt catgcttgtg
    19021 aaattgcctg tgcggtggca cacaatcaag aaaactggcc gctgagtcac agtgactttc
    19081 gaccacgtat ccacgttgta gggaaaggcc tggctgcgaa tccggtggcc tgccatcact
    19141 gcaacaatgc cccttgcgtt acggcttgtc cggttaatgc tctgactttc cagtccgata
    19201 gcgtacaact ggacgagcaa aaatgtattg gttgtaaaag atgcgcaatc gcttgcccct
    19261 ttggcgtcgt tgagatggtc gatacgattg cgcagaaatg cgacctttgt aaccagcgca
    19321 gttccggcac gcaagcctgt attgaagttt gcccaacaca ggcgttacga ctgatggacg
    19381 ataaagggtt acagcagata aaggtggccc gccagcgcaa aacggcagca ggaaaagcgt
    19441 catcagacgc tcagccatct cgcagtgcag cgttgctccc cgttaactcg cgtaaaggcg
    19501 cagataaaat ttcagcaagt gaacggaaaa accactttgg cgaaatctat tgcgggctgg
    19561 atccacaaca agcgacttat gagagtgacc gctgtgttta ttgtgccgaa aaagctaact
    19621 gcaactggca ttgcccgctg cataacgcta ttccggatta catccgtctg gtacaggagg
    19681 gaaagattat tgaagcggca gaactttgcc accagaccag ttccttacca gaaatctgcg
    19741 gcagggtatg tccacaggac cgtctttgtg aaggcgcatg tactttgaaa gatcactctg
    19801 gcgcagtctc tatcggtaat ctggaacgct acatcaccga taccgcgctg gcgatgggct
    19861 ggcgtcccga tgtcagcaaa gttgttcccc gtatcgagaa agtggcggtg attggcgctg
    19921 ggcctgcagg gttagggtgt gctgatattc tggcgcgcgc aggagttcag gtcgatgtct
    19981 ttgatcgcca tccagaaatt ggcggtatgc tgacttttgg cattcctcct ttcaaactcg
    20041 ataaaacggt attaagccag cggcgagaga tattcaccgc aatgggaatc gactttcatc
    20101 ttaactgtga aattggccgc gatattacct ttagcgattt aacttctgaa tatgatgcag
    20161 ttttcatcgg cgtggggact tacgggatga tgcgagcaga tctgccgcat gaagatgcgc
    20221 ccggtgtcat tcaggctcta ccgttcctga ctgcccatac ccgccagctc atgggattgc
    20281 cggagtctga agagtatccg ctgacggacg tggaaggtaa gcgagtcgtg gtattgggcg
    20341 gtggcgatac gacaatggat tgtttgcgga cttccattcg cctcaatgct gccagcgtga
    20401 cctgcgcgta tcgtcgtgat gaagtcagta tgccgggctc gcgcaaagag gtggtcaatg
    20461 cgcgcgagga aggtgtcgag tttcaattca atgttcagcc gcaatatatc gcttgtgacg
    20521 aagatgggcg tttaactgcg gtgggcctga ttcgtaccgc catgggtgag ccggggccgg
    20581 atggtcgccg tcgtcctcgt ccggttgcgg gttcagagtt tgaattgccc gccgatgttc
    20641 tcattatggc ctttggtttc caggcgcatg ccatgccgtg gttgcagggc agcggaatta
    20701 aactcgataa atggggcctg attcaaaccg gtgacgtcgg gtatttacct acccagacgc
    20761 atctgaaaaa agtctttgct ggtggtgatg cagttcatgg cgcggatctg gttgtcactg
    20821 caatggccgc aggaaggcag gcggcgcgcg atatgttaac tctgtttgat acgaaggcat
    20881 cgtgatgaaa tcgttaatta tcgttaatcc ggctgactgt attggctgcc gtacttgtga
    20941 agtggcctgt gtggtcgctc acccttcata acaggagttg aatgccgata tctttttacc
    21001 ccggctgaag gtacagcgac tggactgcat cagtgcgccg gtgatgtgcc atcagtgtga
    21061 aaacgcccct tgtgttggcg cttgccccgt gggggcgctg acgatgggcg agcaggtggt
    21121 gcagaccaat tctgcccgtt gtattggctg tcagagttgc gtcagtgcgt gcccgtttgg
    21181 aatgatcacc attcagtcgt tgccggggga tacccggcaa caaatcgtga aatgcgatct
    21241 ctgtgaacag cgggaagagg ggcctgcctg cgttgaatct tgcccaacac aggcgctgca
    21301 gttgcttact gaaagagaac tcaggcgagt ccgccagcag cgtattgttg ccagcggtga
    21361 gaatcctctg tgaaagaaat ctccgcgctg aaaggcgcgg agaatctatt agattgccca
    21421 accacccgcg taaaatgcga ccagtgcggc agtaatgatc accgtaccaa tattcagctt
    21481 ttgccattcg cgtgcaaaga cgcggcctac gaccagtgtc acaaagccca gcataatacc
    21541 ggtaacgata ttacaagtca gaacgatgaa cacggcacac accaggccag ccatagcgtc
    21601 aataaaatca ttgaaatcca gcttcgagac gttacttaac atcagcaaac ctacgtacat
    21661 cagagcgggt gcagtggcgt aaccagggat caaaaatgac agcggtgata agaacagaat
    21721 cagcaggaat aacgccccca ctacggttgc ggttaaccct gttttacccc cggcggcggt
    21781 tcctgccgct gattcgatat aaaccgctgc gggcgctgcg cccaccaggc cggagaatat
    21841 tgaacttact gagtcactgg tcagggcttt gccgccgttg atgatctggt tgtctttatc
    21901 caacaaattc gcctgaccgg cgacggcacg gatggtgcca gtagcgtcga acactgcggt
    21961 catcaccaat gccagtacac tcggaagtac agttggctgg agtgcgccca taatatcgag
    22021 gctgaaaatc agagacttac catcttcgcc agtcaggctt ggcatcgcca ccagaccgtg
    22081 gtatttcacc gctgggtcaa agattaagcc gatgatcgaa attgcaataa tcaccaacaa
    22141 gataccgccg ggtacgcgac acttctccag accgaagatg accgccagcc ccagcaagct
    22201 catcatcacc gggaaggagg taaacgcacc gagcgccact ggcaagcctt caatcgggtt
    22261 tttgataacc atacccacac cgttagcagc aatcagcagc agaaacagcc cgataccgat
    22321 acctgtaccg tgagcgatac ccatcggcaa attacgtaag atccaggtac gcacaccggt
    22381 tacggaaatg gcggtgaaga tgacgcccat cagaaatacc gcgcccagtg cgacaggaac
    22441 gctaatttgt tgcccgagta ccagactgaa tgcggtaaac gccgtcaagg aaatcgcgca
    22501 accaatcgcc attggcaaat tagcccataa ccccatcagc aacgagccga agcccgcgac
    22561 cagacaggtg gcaacaaaca cagctgcggg aggaaaacct gctttgccca gcattcccgg
    22621 aacgacgata acggaataaa ccatggccag aaaggtcgtt aagccagcca gtacttcctg
    22681 acgaacggta ctgccacgag cggtaatttt aaaataatta tccagcgcgc cctgtggctt
    22741 tggtgcgtcc ggtgtttgta ggatgtctcc agacataatg atgtcctcac gaggatgtaa
    22801 aagttttatt attagttgcg ttcgtacacc agacgaccat caacgtaggt gcggtagatc
    22861 gaacggtcat cgcccaacgt catcatcacg aacaatttgt cgactaaaga aacagagttg
    22921 tcatagcgca gctgctgtag cggagtggcg gtgggttcca tcaccacgaa atcagcctct
    22981 ttgccaggta aaaagttgcc aatcaaatcg tcaaggccca gagatttcgc tccgccgagc
    23041 gtggccaggt aaaacgcttc atatgccgag aggcgatagc cttgtaattg caacactttg
    23101 taggcttcgt tcagcgtttg cagcatgttg aaagtggttc cggcaccgat atccgttccc
    23161 atgcccactt taactttctt ctgccatgct tttttcaagt tgaataaccc gctgccgagg
    23221 taaaggttgg aggtcggaca gaaagcaatg ctggatttgg tttcgctgag acgatcccac
    23281 tctttttctt tgagatggac gcagtgagca aagacacagt ttttaccggt caggccgtac
    23341 tgatggtaaa catccagata accatcatgg tcaggataaa gcgatttcac ccaggcaatt
    23401 tcatctttgt tttcacagag atgggtatgt acccacgtat ccggatattc ctctttcagg
    23461 cgttgcgcca tcgccatctg ttcaggagaa gaagtagggg cgaagcgtgg cgtaatcgca
    23521 tatagcagac gaccattttt gtgccagcgt tcaatcagtt ctttgctttg gtgatagctg
    23581 ctttcggcag tgtctagcag ataatccggt gcgttgcggt ccatcatcac cttaccggca
    23641 atcatacgca tattgatatg actggcggct tcaaacagcg catcaacgga ttgcggatga
    23701 acagtgccaa acaccagcgc cgtggtggtt ccgttacgta aaagctgctt gatgaagaac
    23761 gccgacattt cgcgggcgta ctctaaatcc tcataacgac gttcagtagg gaaggtgtgt
    23821 ttattcaacc actccagcaa ttgttcccca taggccccca ccatttcact ttgcggataa
    23881 tggatatgtg tatcgacaaa gcccggtact atcagtttgc cgcgatagtc gcgcacgcga
    23941 atagtgtcag gaatttgatg ctttccgtct tcccattcgc caaaccattc cacttttccc
    24001 tgtttaatga gtaataaacc atcctcaata aaccgcagcg cagaggcaat ctcttccggg
    24061 ttatcgaccg tacgggtgac atcaataaaa ctgcctcgta ccgcttttaa cgtgtgttct
    24121 cctgacatca ttgactcctt tactgttaat ccatctcttc cgctgaggta ataccaggca
    24181 gaacgttttc ctgtcggtag ccaccaggga ggataatatt taataaaatc gcagttaacc
    24241 cgccagcaca aatagggttt tcaactaata cataaataga ggctggcaat attttaaaaa
    24301 tttcgggatc gtaggagacg ccaagtccta aacctaaaga agtggcgaca ataagtgttt
    24361 ctcggcgctt taaaccgttg gtgatgatga tgcgaatccc agcgatggca atcatggaaa
    24421 acatcaacgt cattgcgcct cccagaactg ccgagggaat ggtcgtgaag aagccgccaa
    24481 tcatcggaaa caaaccgagg ataaccagca ttaccgcgat ggttcgcccg acataacgtg
    24541 aagcgacgcc agtcatctga ataaccccat tattttgcgc aaacgtggtt aagggtaatg
    24601 acccgacagc ggaggcgata acagaaacca gaccgtctgc cagcacgccg cctttcagtc
    24661 gggactgata ctcttctccc tgaatgggtc ggcgggaaac cattgccgtg gcggtgatat
    24721 cgccgacagc ttccagcacg ctaagcagat aaatcgtgcc aaccaccagg aactgatgga
    24781 agctaaaact aaagccgtat ttgaacggat gtgggatggt gattaacggc aaattgcgca
    24841 tactgctgaa atccaccatg cccaggcata acgacgcgat ataaccgacg catagcccaa
    24901 tggcgatccc tcccatgcgt agcaacggac tgcgacagca gttaaagccg atcaccacaa
    24961 tcaaaaccaa taaaccaacg ccgagatgtt cgtaattgcc gaaggtaccg ctgcttttgg
    25021 cagcaaatcc tccaccaaaa tcgataatgc cgactttaat caggcttaag ccgatcatca
    25081 gtaccacaat accgctgaca gtaggcgtaa taacccgacg taaatagggc aagataaatg
    25141 aagatccgac aaccagaaat gcgccaacga aggagacgcc gaggagcgac gacatgatta
    25201 actcttcgtg aaaaccgtcg cttttcatgc tgctgcccag cgcaatcatg accgtaacaa
    25261 atgaaaaatt gactgactga attgagagta ggccagaacc gacgatgcca tagcggttta
    25321 cttgtaacca ggtaccaata ccagaggcga tcatcgccat tgaaacaaga taggcagttg
    25381 tttcagcgga aagctgtaag gccgccccca cgattaacgc gggggttacc atcggaacga
    25441 aaattgccaa cagatgggta atggcaccaa cgagagcctg atgaaaggga gggcgatcct
    25501 ccagttcaaa tataaggtca gaacctgcat ggtttatatc agacatccct tcactccttt
    25561 taaagtaaat ttgatcatct gaattagtaa aataaattcc cacgaatttg gggcgcagga
    25621 cgccttgaga acggcttatc tcattttcgc agtcactatt tagtcatcgt ccaaacagag
    25681 aaacaggccg ccgaagcggc catttattat ttatatcttt tccagcgcgg tgagtatttt
    25741 ctccggtgtg aaatgccatt cgcgtaacca gatgccgcat gcatcgtgaa ttgcggtagc
    25801 aatcgccgga gctgcgccat ttacaccgat ttccgagatc gatttcgccc cgaacgggcc
    25861 gactttatcg tcgctcggca ccagcactgc gcggaaatca cgcggaatgt caccaatttt
    25921 cggtgcgccg tagctgcgta aatcacgcgt taacgggtga ccttcggcgt cataaatgat
    25981 ctcttcgctc atactgtggc cgatagctcg cagggtagca ccgtagattt gtcccaacgc
    26041 taactctgga ttgaccggtg taccgcagtc cagcaaggcg tagaatttat ccaggcggat
    26101 ttcacccgta cgcgtgttga cggcaacttc agcgaagttt gcgccatacg ggaaggcgaa
    26161 atcaggcgtg atataactgc cagttcccac cagtgaacca aagccggtgc cggtttcgcc
    26221 tttatgggca atatccccga aactaacttc gcctttcttg ccgcgcacga cgcccggcgt
    26281 tgctagttga acatctgcca ctggctcacc caacatttgc gcgccgtgga acagaatttt
    26341 ctcccgcaga ttttccgctg ccaaacgcgc cgcgttaccc gagaagcaag taccggacga
    26401 ggcatatgcg cctttatcaa acaacgcatg atcggtatca ccggagataa catgcacgtc
    26461 ctgcggtggg cagtgcagca cttctgctgc cagtttcgtc accacggtat ccagaccagt
    26521 accaatatcc gcaccgccag aatgaacgat aaaggtaccg tctgattcca gtttgatcat
    26581 gcagttagcc tgatcgatat ccgggatccc tgatttctgc atgataatgg cgacaccacg
    26641 accgatgtgc cagtcaccat tttgtggttt tggtgaagac cattggatca tctcgcgccc
    26701 ctgacgcagg atctcttcca gtgcgcagct ggcggcggaa ggaacggagg tcggcgcttt
    26761 accttcaccg attgcaccga gaattttcag ctcttgccct tcgtgtaccc ggttacgttc
    26821 gataatttcc agttggtcga tctgtaactg ttcagccagt tccgctaatg ccatggtgat
    26881 agcgaagtta cctttcggtg cgccataacc ctgataagca ccatttgggc aaatgttgct
    26941 gtagtaggtg gtgacctgga aatcgacgtt atcgcacgga tataacggca gcgacagcgc
    27001 cggtccgtta cacggtacgg tgagtgagtg gttgccgtaa gggccagtgt tggcgcggaa
    27061 atccatcttc actgccgtca ggcgaccatc ttttttcgct cccagtttga cggtgacttt
    27121 cgcgacgtga cgagaggtgt tagcaataaa ctcttcttca cgggtgtagc ggaacagtac
    27181 cggacgcccg gtcacgcagg ttgcccaggc gcacacttct tccagcagga tgtcctgttt
    27241 ggaaccaaaa ccgccgccaa ctcgctcttt aatgacatga actttatgct gtttcatgtc
    27301 cacgaggcgc gcgacctggc ggcgtaagtg ccatggtacc tgggtggagg cgtggataac
    27361 cagacgatcg ccgtccatcc gggtaaagca gatatgtgtt tcagtcgggc actgctgcgc
    27421 ctgcgttgag ttataggttc gctcaatgat cacatcggca tcggcaaagc ctttgtccat
    27481 atcgccaata tgaccatgaa tactagcggc gatatttttg cgagggcgag aaccgatcgg
    27541 gaagttgatg atcatatgct cgccgcgctg ggctgcatgg ctgttatcgt cttccagagt
    27601 atctggcgca ccagcaacat acaccaccgg ttcatcgtgc acgacaggcg catcttccgc
    27661 cattgcttcg tcgatcgaca ttaccggctt aagcacttca tattcaacct cgatgagttt
    27721 caatgcttcg agcgcaattt cttcactttc agcgaccacc gcagcaacgc gatcgccgac
    27781 gtgacgcatt tttttgccga acatacggcg gtcaagcggt gacggttccg gtgcgctctg
    27841 accccccggg gtatagtaga tatcagggca attcaggtga gtaataacgt gaacgacgcc
    27901 cggtaaggct tcagctttgc tgacatccag atgagtaatc agtgcgtgag cgtgtgggct
    27961 acgtaacatt ttaatgacgc aggcgtcagc cgttacgcgg tcttcaacat agcagggttt
    28021 cgcctgcacc attttcgcgg catcagtttt aggataatgc ttgccaatga cttcaaggtc
    28081 gtcacggaaa gtcggagcga tatcaatggt ggcctgcgga ttatttttac gtgcaaccgc
    28141 cagttcaatg acctgatagt attgctgcca gccagcatcg cggctgaaca aaccagaaag
    28201 cgcgtcgtcg atctcttcgc gggtaggtgc ggcgatgcga tcgagaagat cggtgataat
    28261 cagagctgca gctggatcgt tataaccaga ctgcaccacg ccaacatcaa ccattgcctg
    28321 ttgaaccaga cttaactcgt tccatttgcc cagagattct gcggtacgaa tatctgcctt
    28381 ctctaactgt gcggcaataa gcaaggacgc gttaacgata ttaccgttaa agatgattgc
    28441 gtcagaaccg gcaaacccga aaccatcatc actgttgcgt acagagtgca ttcccatgtt
    28501 aaacaacagc ttttgcacgt tttcgcctgg attaacggtt agctcctgag gcgcgccatt
    28561 taaagtaaag tggatgatca tacggcttcc tccccagcct gttggcagtc ggcatacagg
    28621 tcggctacca gaactcccgt gatatagcgt ttataggcca cgctgccccg caaatcttcc
    28681 tgcgggaaaa tggcgttggc gacagcctgt tcaagtgcat tgccttccag attttgtttt
    28741 tcgacatcat gcagacgcag tgctttactg gcgacgccat ccagcgcaat tcgcataccg
    28801 tcgtggtctg tcattgcaac ggctgccgtc acgacggtta aacctgcctg agaacggcta
    28861 attttgcggg tcgcacaggt gcgatacgga tctttaatga taatttcggt tagcaggcga
    28921 tcgcatgggc aggccaggta gtcctcgatt gacagcgttt cgccgttgcc aaaaaccagt
    28981 tcagcatcca gtgccagcag gacgggaagc agcactgact cttcctgccg ggcggcgatt
    29041 tcaccaccaa tggtcgactg attacgaaca tggcgtgagt aaacaaaacc gagggcttca
    29101 cgcagtgctg caggaataaa tcgcgcatca cgcagtggct gcaagcgaga cattgcgcca
    29161 atccgcagtg caccgttatc ccagtcaacc cagtccagtt ccagatcctg taaggaaatg
    29221 gcaatctttt tatcggtacg ggttggtgta gcattgagtt tgctgccccc ggcgaaccag
    29281 acggcttcat cctggtagcg gcgcttcagt tccagcgcct gttcgacaga gtcgggcctg
    29341 aaaaattgtt caatcatctt ctttccttat ttaaccctct tcctgcggga agagggcatc
    29401 tgtcatttat gccagcgcat ccatccgacg ccacatactg gcagcggctt ttctggcttg
    29461 tgcataaatg gaatcgcaat cgaagttaaa ctgacggtct tcatagacca tcacaccatt
    29521 gaccatcacg ctgtgaacgc tgcctgagcc cataccgaaa gcgatatgcc cggcgatatt
    29581 gtctgccagc agcggcgtcg gcgagttgta atcgcaaatg gttaaatcag ctttgtaacc
    29641 ggcttccaga agcccaaatt tcgcgccaaa gttgcggctc atcagttcgt taccgttagt
    29701 cagggctttg gcaaaactgt caggccacag cggaccaccc gcatcgcgat gtttaaagaa
    29761 ggcaaatttc atctcttcaa acatgtccga accaatgccg tccgttccca acgccagatt
    29821 gcggatgtcg ctaaggtgat ggttgtagcc gacatggttg ttcatgtttg aacgggcgtt
    29881 atgcaccagg aacgcatcgc gctgattgag tagggcgatg tcatctttcg acaagtacag
    29941 cccatgagcg accagcgttt tgctatcgat gagatcgaat tgcgccagtc gtgccagcag
    30001 gtctttgccg taccagtggt gactgtagga aacgtcgtaa aggtcttccg cagcgtgaat
    30061 atgcaaacca cggcctgtgg cgttcacggc ttcacgtagc atctccagac cggcatctgg
    30121 cacggtaaac ggcgcgtgag caccgatatg tgcttccacc agatacggct cggttgctgc
    30181 tttcttcgcc tcatcaatct gacgggcgaa gcggatgttt tcttctacac cttcctgcaa
    30241 ctctttgatg ccgttgttac ggtcagtagt ttcaaaacag gtcatcgcgc gcaggccaac
    30301 ttttaaaaat gcgtcgcgca atgtggagag cgacccgccg atatacgccg gagaggcatg
    30361 gtgatcgata accgatgtac atccgctctt aatcgcttcc agggaacaaa tcagcccgct
    30421 gtaatagagc gactcttcat caagggcgcg atcgagccgc caccagagat ttttcagcgt
    30481 tgagatgaaa tctgggctgg gggcgatgtt tgccataatt ccgcgggaaa gccccgagta
    30541 aaaatggttg tgcgagcaga caattcccgg catcacaatc cgaccatgca tctctttgta
    30601 gctggcatcg gggtagcgtt gcgtcagggc atcgccgata gcgacaatca catcattttc
    30661 gatagcgata tcaacgcctt cctgcacttt cgccgggtgt aactgcactg cggtgacatt
    30721 cttcagaatc aacatgatta cacctccacg cggcccagca gataatgatg atgctggtgg
    30781 acatggctga tgatgcggca catatcgttc agctccggtg gtacgttgtt gaactgacct
    30841 tcgctgtcga tgtttaacac ccagctttgg ttattcagtc gtactcgtac ccggcaatct
    30901 tccacaagga agcctgggtt gctgctgtta tcaaagtctt gcgccaggct gaaaacggtg
    30961 attttgtctt tgtatggttt accgttccac ggacagaact gagcgcagtt gccgcattcg
    31021 ttacagtaag cgtcgaggtg cagcgtctgg aatctattct ggaagcctgg gaccgcaatg
    31081 gagacgttgg cgcggttcgg gcagacatcc acacacttgc tgcaaacgta gttacattca
    31141 aggcagcgag cagcttcctg cgcgacaaac gcatcacgat cgtcactgtt caccagcgtg
    31201 atagagatat cgcctttacg ttgatagatt tccgccggat tgacgttgtt ccagtattta
    31261 tcgttctggt gggaacggat attttcccga ctaaggatgg catcggtcgc ccgacgcgcg
    31321 gttccgacag cagcgacaat ggaggatggt ccgcgctgta cgtcgccgat cataaagacg
    31381 tcagtcagac gagtttcgcc attatggtcg acgtctggcc agccgttttt gtccagcggc
    31441 acgcccatcg cattcagggc ttcagtatcc tgctgttcac caatggcggt gatcaggctg
    31501 tctacatgca gtgttactgt ttcgttggtt tcaaccggac gacgacgacc tttctcatcg
    31561 ggttcgccaa gcgacataac gcgcaaggtt aaggtgccat cagcatcgaa acgttccgga
    31621 ttattcagga aacggaactc cacgccgtcg tgcaacgctt cttcatactc ttcgcgccat
    31681 gcgggcatct cttgcagtga acgacggtaa acgaccgttg ctttttctac gcctggaacg
    31741 cgtaacgccg cacgagcgca gtccattgcg gtgttacccg ccccgacaac gaccacatgt
    31801 ttgcccagct tgagcgctgt acccttgttg tattcacgca ggaaggggag tgatttcaag
    31861 acattttggt tgtcgcccgc cagtttcaca ccgctatttt tatctgtgcc ggtggcaatc
    31921 agaacatagt ggaagtcctg attttttaac tgctcaacgg ttaaatcggg tgagcagcca
    31981 tactcaaatt tcacgccgtg agcggcaaca aaatcgatat cgtgctgaat taactcagcc
    32041 ggaattcgga actgaggaat gatatttttc accacgccgc ccgcattggc ttcgcgttca
    32101 aacagcgtaa ccggatggcc cgctctggca aggaagtaac ctgctgccag acccgccgga
    32161 cctgcaccaa tcacggcaac cggatggcgt gaaccagaac cggctggttt gtgccagcgt
    32221 tgcttatatt catcccaacc tttttccagc gcgacttttt tcagttcgcg gatattcagc
    32281 gcactgtcgt aatccaggcg ggtacagttg tattggcact ggtgatcgca aatatgaccg
    32341 gtaatggcgg gcagagcgtt gcgttggtag atgagttcca gcgcgtcggc atagcggtgt
    32401 tcgccaagca gacggatgta ttccggaata tcttgcttga tagcgcaggc agtaacacag
    32461 ggggcaacgt agcagtcggt cagcggcagg tcttctgcca cttcaatacg ctcttctggc
    32521 ttccagtgtt tctgggtgta ttccatggtt aacgcatctg ctgccagtct gttcagtcgt
    32581 tcgacgtcaa catggtcaag tccccaggcg tcggagcctt ccagctcgcg catgcaggca
    32641 cttaagcgca aatagccgcc aggtttcagc aggtcggttg ccatagtaat agggcgaata
    32701 cctgtatcaa aaatatcgcg gatagtcagc tgactggcac caccggaata agaaatgggc
    32761 agtttgccgt caaaggcgcg agagagaact gctgcaacat tgatggagag cgggaacagc
    32821 gcacggcctg acatatacat ctcttcacca ggcagtgcgc ctttattgtt gatggtgccg
    32881 agagtgttag tcagttttac gccaaagccg agtgattttt cttttgccag tgccatcagg
    32941 cgttccagca tttccagtgc ttgcgtcagc ttgaggtcgt gatcaaatga ctcttctttt
    33001 aagcctatgt aaccgaaacc gcagacatcg aggatctcac gaacacgcgc gtaccccagt
    33061 aaggtcgggt taagtttcac aaaggtgttg agcccttttt cttccagcat gtagcggcaa
    33121 atggcttcga tttcatgcgg aggacagccg tgcatggtgg agagggtgac gccatgcacc
    33181 atactggtgg ggatgcgagc gggtaaggct tgcaagcttt cgcgtttttc ctgcaatccg
    33241 tggcgggcta aaaatgcgtc atcctggagt aatttattca gcgtatcgcg atattgcgcg
    33301 aatttcggat ggtcagatgc gtccatcata ttgtcgatga actgttgcat tggcggttgc
    33361 ttaatacctt cgaggttgta accgacgctc atattaaaga tgaacgattt accggaatca
    33421 gaaggttgga acatcgcttc gagaaggtgc agggcaaacc aggctttgag gtattcatcc
    33481 caggctttaa gcagggtaaa ttcggtagac cattcggtgt taaagcactc gtcttcggca
    33541 tcaatacagg gcttttccag ctccaggcgg tcaagaattt ggacggtttt tagttcgatg
    33601 aaacgtccgc cagtcagcca ggaagtgaca atattttgcg cgagctgcgt gtgcgggcca
    33661 gcggcagggc cgacgggagt ggcacaggtt tcaccgaaga cgctaacagt tttacctttt
    33721 acaggtgagt aaaactgttg ctcgggaata ccaaagattg agcgttgttg ttggtattca
    33781 tcaaatatgc gcgtcaaaag ttcctcaaac ggaatgggac gcataatatc ccccataacc
    33841 ctctccttag cttatacaga tatcattgag tgaccggtgt tgagaaatcc ggtggtttag
    33901 ggggatagag caacaatcgc accactcttg ccaggtttgg cgggaaatat aaaaaaatgt
    33961 gaggcggctc gaaacttaag ggctttttgt gaatgaactc acaggtacta aatgagaatt
    34021 aaaatcactc ctgaaaatta ttattgccat cccatatatc actttttctc ttttctcaaa
    34081 atcagtgatt ccttatcatt atgataaaga gtgctggagg gattaaatat ttgaacgcaa
    34141 tcgtttaact acgtttttta aattttagta tacctttttt gcgctgtaat aaagtcgtcc
    34201 ggagtatcaa tatctaaaat tatttctgca ttttctactt cgacggaata atgagctccc
    34261 ataaggagtg cctgacgcat attggtgata ttggggcgct tgattgcctg catcaggcat
    34321 ggttttgaca ctaaaatcgg atggccgggg atgccattat ggagtggcag tattgcgcca
    34381 tcatttcgta acgaccagat ttttctgaaa atatcgatgg tgagggttgg catgtcaccg
    34441 tgggtgagaa aacaatgttc tgtttgcact gcgggtactg cggcctttac tgatgttagt
    34501 aaaccctgcg cataatctgg gttgtggata atagtgatat tgctctggtt cgcataacgt
    34561 tcgtgcagtt cattaccacg atagccggtg actaaaataa ttcggctaca aaactgcaac
    34621 gcatttttga tacttgtatc aagaattgtc ccctgttgcc agggtaacat cattttccat
    34681 tgccccattc ttgatgataa tccggcagcg gtaattatac agtcgatggc tgacatagtg
    34741 gttccattat ttataggggc taataacgcc tgataattgc gttacttcct gatggattaa
    34801 gttggggata atttatccca gagaggtcat aaagactcgt gaaaagtata gttgacccat
    34861 cggcattatt cattgattta ggtgcgcaga aacgtcccac tgtgatttct gttgttggtg
    34921 ctggggggaa aaccagcttg cttttttggc tggcagaact gctccaggct agcggcaggc
    34981 gtgtgttaat cacgacgaca acacatatgt ttatgccaac gtctcactgg cccgtggttt
    35041 tctgtcgtga tcccgccatg cttcctcatg cgtctcttac atcccccatt tcattttgtt
    35101 ttcacagctg gaaagcgaac ctgggaaaag tgcagggatt tacgccagaa gcgattgatg
    35161 cactggtgca acgaccagag tgtgacgtaa ttctcattga agcagatggc tcgcgtgcaa
    35221 tgccgttaaa agcgcctgat gagcacgaac cttgcatacc caaaagcagt tgctgcgtga
    35281 ttgctgtgat gggaggacat attttgggcg cgaaagtgag cacggaaaat gtccatcgct
    35341 ggtcgcagtt tgctgatatt accgggttaa cacctgatgc aaccttgcaa ctgagcgatc
    35401 tcgttgcgct ggttcgccac cctcaggggg cgtttaaaaa cgtaccgcaa ggttgtcggc
    35461 gagtctggtt cattaaccgt ttttctcaat gtgagaatgc gattgcgcaa agcgagctcc
    35521 ttcaaccgct gcaacaacac gacgtagagg caatctggct gggcgatata caagaacatc
    35581 ctgcaatcgc gcgcagattt gtgaattagc ggcccgggta ttccgggtta ctgaaaggat
    35641 atccattgag gttggtatga atattttcac agaggctgca aaactcgaag agcaaaattg
    35701 tccgtttgct atggcgcaaa ttattgatag ccgaggctcg actccccgcc attctgcaca
    35761 aatgttagtg cgcgccgatg gctctatcgt cggtacaatt ggtggcggaa tggttgagcg
    35821 gaaggtgatt gaagagtcgc ttcaggcatt gcaggaacgt aagccgcgat tattccatgg
    35881 acgtatggct cgtaacggtg cggatgctgt cgggtcagat tgtggaggtg cgatgtcagt
    35941 gtttatcagc gtccatggta tgcgtccacg tctggtgttg attggcgcgg ggcatgtcaa
    36001 ccgggcgata gcccagagtg cggcgctatt aggatttgat attgccgttg ccgatattta
    36061 tcgcgaaagc ctcaatcctg aactattccc tccatcaacc acgcttctcc atgctgagtc
    36121 gtttggtgcg gcagtggaag cactggatat tcgccctgat aattttgtcc tgattgccac
    36181 gaataatcag gatcgtgaag ccctcgacaa actcattgaa cagcccattg catggttggg
    36241 gttgctggca agtcgtcgca aggttcagct tttcctgcgt caattgcgtg agaaaggcgt
    36301 ggctgaagaa catattgccc gtttacatgc gcccgttggt tacaacatag gtgcggaaac
    36361 gccgcaggag atcgccatca gcgtgctggc agaaatatta caggtgaaaa ataacgcgcc
    36421 gggtgggctg atgatgaaac cttctcatcc ttccggacac cagctggtgg tgattcgcgg
    36481 tgcgggagat atcgccagtg gtgtggcgct acgtctgtat catgcgggtt ttaaagtgat
    36541 catgctggaa gtggaaaaac cgacagtgat tcgttgtacc gtggcgtttg cccaggccgt
    36601 gttcgatggc gaaatgacgg tcgaaggcgt cactgctcgc ctggcaacca gctctgcgga
    36661 agcgatgaaa cttaccgaac gcggattcat ccctgtgatg gtagatcccg cctgttcatt
    36721 gcttgatgaa ctgaaaccgc tttgcgtggt ggacgctatt ctggcgaaac agaatttggg
    36781 aacacgggca gatatggcac cagtaacaat cgcgcttggg ccgggctttg ctgcagggaa
    36841 ggattgtcat gcggtaattg aaacaaatcg cgggcactgg ctcggtcagg tgatttactg
    36901 tggttgtgcg caggagaata ccggtgttcc tggcaatatt atggggcata ccacccgacg
    36961 ggtgatccgt gctcctgctg caggcattat gcgatccaac gtgaaattag gcgatctggt
    37021 gaaagagggc gatgtgattg cctggattgg tgagcatgaa attaaagcac cgttgacggg
    37081 gatggtgcgt ggcttgttga acgacggcct ggcagtggtt ggtggtttta aaattggtga
    37141 tatcgatcct cgtggtgaaa ccgctgattt caccagcgtt tctgataaag cccgggcgat
    37201 tggcggcggc gtacttgagg cgttaatgat gttgatgcat cagggcgtga aagcgacaaa
    37261 agaagtgctg gaagtggctt aaatgaaaat ggccggatgg tattacccac ccggccttaa
    37321 aattactgac aaatcaccgt tccggttttg ccttcaatcc cttctttcgc tttgctcaat
    37381 acggtaatca gtgcttcacg acctgcgcga gagcgagcaa acgaggcggc agcttccact
    37441 ttaggcagca tagaaccttt cgcaaaatga ccttcttcga taaagcgttc agcatcactt
    37501 aatgacaggc gatcgagcca ctgttcattc tctttaccga agttaatggc cactttttct
    37561 accgcagtca gaatgatcag catatcggca tcaatcattt ctgctaaacg ggcgctggcc
    37621 cagtctttat cgataaccgc gctggcacca cgcagatggt taccttcacg aataacagga
    37681 ataccaccac cgccaacggt gatcaccacc tggcctgctt ctaccagcgc ttttaccgtc
    37741 tctttttcaa tgatatcaac cggttttggc gaggcaacaa cacggcgata gccgcgacca
    37801 gcatcttctt tcagagtgta accctgtttt gtcagttgct ctgcttcttg ctcggtaaag
    37861 aacgaaccga tcggcttggt cgggttgagg aatgccggat cgttagcgtc gacttcgacc
    37921 tgagtaacca gcgttactac aggtttatta atgccccgag aaagcagttc ttctcttaag
    37981 gcgttttgta gatcgtaacc aatataaccc tggcttaagg caacacagac agacatcggc
    38041 agcattggcg aatgcgcttc agtttttgcg gcagcttcaa aggcctggtt aatcatgccc
    38101 acctgcggcc cgttaccatg agtgacgata acttcatgtc cctgggcaat taaatcaaca
    38161 attgcctgag aagtaatttt taccgctttc atttgtccgg ccaggtcatc gcccagcgca
    38221 ttcccgccca gggcgagaac aattttctta ctcatttttc ctcactcatc attcttgatt
    38281 gtgtatagat ccctggcagt ggttagagca cgggagggac aaacggtttg cgtcgcagga
    38341 aacgacctcg cccggctttg cctgtaaagg tgccgtcaca gaaaatcgtt tcaccacggg
    38401 ataaggttct gacaatcgcg ccctgacagg taaaaccctc ccatggcgag tagtcggcgt
    38461 tgtcgtggag atggcgatgc tgaatttgtt ggctctgacg tgggtcgata atcaccacgt
    38521 cgccatcgga accgggcgct aataatcctt ttggcggcca caggccaaac agcctggcgg
    38581 gcattgcgct ggttaattca acaaagcgtt ccggtgttat acgtcccgtc atcacgccac
    38641 tggaaaacag taactgcatg cggttttcca caccgggtaa gccatttggg cagcgactga
    38701 aatcgccttt agaaatttgc aggcgttgag ccatcgagaa ggtgcagtga tcggtcgcca
    38761 ccacgtcaat cgcaccatcg ctgatgccac accacagttt gtcctgctcg cgtacgttac
    38821 gcagcggtgg gctaagaatg aacttcatgc cgtcttctgt atcgtaactg cgttcgtcca
    38881 acaggagata ttgtgggcag gtttcaaccc agactggctg gtgattcgca cgggcaagac
    38941 gcagataatc cagacctaag ccgttagaca ggtgcacgat atagagcggg gcgttaccgg
    39001 caatttgtgc caggttaatc atgcgggcga tggcttccgc ttcgcattcc agagggcgac
    39061 tcaaggcatg atagcgcggc gcggttaacc ctgcggcgat aaattccgcc cgcttgctgg
    39121 cgatagccgc atcattttcc gggtgcacgg tggtcagcgc gccggattca tgcagacggc
    39181 gtaatgcctg caaaacctcg tcatcgttga gtttgtattg ataggttaaa tagagtttaa
    39241 aactgctcag cccttcctcg accatcatcg ggatttcgtc gaggatggcg tgattaatgt
    39301 gctggatcac accgtgaaag ctgtaatcga tgaccgcttt atgggcggca taaccacgat
    39361 aaacctccag ttgatggcgt aaccgacagc cgtttgggcc aaatcccata tggtcaataa
    39421 tggttgttgt accgccacac gcagctgcgc gggtaccggt aaaaaaatca tcacaactgc
    39481 gcgcgatgcc gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt
    39541 aacagccagt ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact
    39601 ggcgaacaat cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga
    39661 cagtgccgtt tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga
    39721 ggggaataag tgaagagtaa ttattcggtt gccagccagc ttaacgggat tgcagcgtac
    39781 atcgcggcac aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca
    39841 ggttctttac ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg
    39901 ccgttagttg agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag
    39961 gcattcacca gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag
    40021 cattcggttg ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca
    40081 accgcgttag ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag
    40141 gtttcgcccc aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa
    40201 cggcttgggg atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg
    40261 tcataaccca gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa
    40321 atggcgttgt caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata
    40381 cgaatttcca tacgaccgcg ctgaccacgg tatacctggc agtcggttgg ttcggtactg
    40441 accacaaatt ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg
    40501 cagtcttctt cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg
    40561 tctttaatga ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta
    40621 ccgcgaccac cgatcagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt
    40681 ttgatgttgc caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg
    40741 tggccgatat aaccgagaac gttacccatc gggtcgattt caactttatc gaagccgact
    40801 ttttccatct cttctttaat acgatgtact acccgtttct cgtcgcagct ttcactggga
    40861 atagcaacca tgtcgcgcag gaagcgagtc atatccgcct ggtaatcttt tgctttttca
    40921 agaatcagtt tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg
    40981 ttttgcaggg agcagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag
    41041 acaacttcgc ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg
    41101 gcatctttgt tcagcgccag tttttccatc aggctttgac gttgcgggtg ataatgaacc
    41161 gctgcgagaa cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg
    41221 ccgtacggat tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg
    41281 aattgggtgg cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc
    41341 aggcctgcca tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg
    41401 gagcgataaa tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg
    41461 ctatagacgt cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc
    41521 agcagaacgt gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc
    41581 agggttgcgt agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg
    41641 tcctgtacca cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg
    41701 tcatagttca tatctgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt
    41761 tcctgagcag aacctttcgg catgtaaatc accgcattct gtccgagttg ctgcgctgcc
    41821 cacgccacac cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg
    41881 atggcatttt tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac
    41941 aataattgag cgatagcgta cgcaccgccg agcattttga aggcgttcag accgaatcgt
    42001 tttgattcgt ctttgacaag aattttcttc accccaaaaa ggtttgcgag atcgtccagc
    42061 gcacaaagcg gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg
    42121 gcctggcttt gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata
    42181 tcaatcttca atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc
    42241 gaccagctga tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc
    42301 tcgagcagtg cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa
    42361 ggcttccagc tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc
    42421 acttcccctt ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg
    42481 gcttcgccat cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc
    42541 gcgttctgtg ccagacactg tttttccagt gcttttaagc cttcgtgatc gttagcgcgc
    42601 agcaattcgg tacgctgttc catcactttg taaggtgccc atgacttcgg ataaacgatg
    42661 tctgcatctt tgaacgcttc ttccatgctg gtgacctgac ggaagctacc gccagatgct
    42721 ttagcattgt ttttcgcgac ttctactaca tccgggatca ggtcgtagcc ttccggatgg
    42781 gccagggtga catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag
    42841 agcggtttgc catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg
    42901 ttttccagtg aaccaaagtg ttcacgtaac catgccaggt cagccattga ctgagtcggg
    42961 tggtcaatat cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg
    43021 taaccgtcat caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac
    43081 atatcgtcgc gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc
    43141 acggtttcgc cgtgagcgat ttgtgatttg ccttcatcga gatcttgctg tgcaaggccg
    43201 agcaggttaa gcgcggaagc ataagagaag ctggtacgag tggagttgtc gcggaatacg
    43261 gaaataccta atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt
    43321 aatgctgcgg caacgtccag tacttgtttc agttcatctg gagtctgttc ccacgttaac
    43381 aaaaaatctt tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta
    43441 acagttttca tcataaagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt
    43501 gacttgccaa agtgattggt gttttcacag ctatacctgg gactaacaat aagtgtgcca
    43561 gaattatcca cggggctgca aaagactaat ttgacggata atgtgtgggg ttgatcacaa
    43621 attgatgcca gcttccggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt
    43681 tatttatttg ctgctaagta tcagaaataa gataaaggga caggctatcc ctcgtcagtt
    43741 tgataaactc accgccataa ttctcatatt gatatagaaa tgtgatatct cttccataat
    43801 ttcattagaa agtgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat
    43861 atgatcatga tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa
    43921 ggtaaagcta tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt
    43981 cagcgttttg ccagaatgct tgccagcgtt ttgcagcttg aggttgagat cgttgatgaa
    44041 aacttgtatc gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc
    44101 ggcaactcac gcctgctctg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag
    44161 tctcgcttcg atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca
    44221 tttctgggta cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc
    44281 gttacccacg agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt
    44341 cgccatatat ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac
    44401 atcagtaaaa tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat
    44461 gatgaaagtc gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtgcaa
    44521 aataatatta ttgggaaacc tatccgtttc agaccattaa catttgagag taattttacc
    44581 catggacata tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa
    44641 ttgcataaca ttcagggccg acaattattt ttaatggcgt ttcaccaatc gcataccagt
    44701 ttttctgtag caaatgcacc tgatgaaccg catattgaac aattggttgg cgagtgccgt
    44761 gttatgcggc aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg
    44821 gtggttggtg aaagcggcac gggtaaagaa gttgtcgccc gtgcaatcca taagttgagc
    44881 ggaagacgga ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg
    44941 gagagcgaac tgttcggtta tgttaaaggc gcatttactg gcgcttctgc caacggtaaa
    45001 acagggttga ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg
    45061 ccattaatgt tgcaggctaa attactgcgc gctattgagg cacgtgaaat tctgccgatt
    45121 ggtgccagta gcccaataca agtcgacatt cgcatcattt ctgcaactaa ccagaatttg
    45181 gcccagttca ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc
    45241 ccgataactc tgccaccgct gcgtgaacgt caggaagata ttgaactact ggtgcattac
    45301 tttttacatc tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc
    45361 gtcgaaatat tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg
    45421 gaatatctgg ttaacgttgt tccttcaggt gaagttatcg acagcacgct attgccgcca
    45481 aatctgctga ataatggcac aacggagcaa agtgatgtaa cagaggttac tgaggcacac
    45541 ctgtcactcg atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc
    45601 gaggcgcttt cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt
    45661 gctacgctct atcgcaagat taagaaatat gagttgttaa acacataaca tcagtgaatc
    45721 cggcctgaat tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt
    45781 tacaatcatc tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc
    45841 cgtaatggtt aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag
    45901 gccaggcgta caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg
    45961 agaaagtttt ccgcctttcg cttcaccttc cagcgtgcgg atctcttttc cttcagccca
    46021 ggcggcaagg tataagcaac tgtctattgc tgtgccgtcg accagcaccg tacaggcacc
    46081 acattcaccc acgcagcacc cttgtttgac acttagcagc ccttgttcgc ggagtaattc
    46141 cgagagcggc gtgcctggta cggcatgaag ctgaaaaggc atcccgttaa tggtgcattc
    46201 gatggtaatt gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg
    46261 ctaatcactt ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc
    46321 catgaagaac gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc
    46381 aggtttaatg gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc
    46441 gcggcaacac caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt
    46501 gcggcacagc caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg
    46561 ctgcccacgt gttctttcgg ctggggcgga aaatgaaagg cgacaaggat ttcgtcatgc
    46621 tcaagagaca ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg
    46681 cgtggggagt ggatctccag tttcgcgtca taaatgagcg ttggcgtggc agaatctgcg
    46741 ctggtggcac cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca
    46801 gcaatggacg aggccgcagc acataacgcc gggagatgac gttgagttat agaatcttct
    46861 attagctggg taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta
    46921 attccccgca gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga
    46981 tggtggagct gaatcagtac gtcagtgcca ccggcgagca gtttggcctg cgggttgtca
    47041 gccagcaggt tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac
    47101 atgatgttat cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt
    47161 cagcggcagt gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc
    47221 ggcaacagga attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc
    47281 attgatttcg acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta
    47341 atccagcaga ttggggttac ggaccacgcc gcttttcgca tcgatgatca tctcttcaaa
    47401 tagcgcccag ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagtagcgg
    47461 attaagaata tggcccgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag
    47521 cgcaatatcg accgtcagat caacaaaggt acagccgaac gccggtgggt tagtggtggt
    47581 tttgatggag ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat
    47641 cgccaaatct tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc
    47701 ttttatcagg gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat
    47761 tttctctttt aataacagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg
    47821 tgaggcaaat gcgccgggat cgaacggcgt aacgtcggta tcttgtgttg aaataacgcg
    47881 aacgtcgctg accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc
    47941 accctgaccg atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag
    48001 aaggcgcgcg cctgctattt ctacgccgac aggccaggtg ttagaggtgt agctaaaaca
    48061 ggcgacgcca acgccgcggc gtaaattgcc ttgctggttc tggcactctg cacggcgttt
    48121 ttcccattca aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat
    48181 acgtttgccc gtgagcggat tagcatctcc ttcgcgtgag gcgttgcgta aacgaatttc
    48241 aacaggatca atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac
    48301 gacttgtggc gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt
    48361 ctttgaactg taagcgtagg cacaacgagg ataaaggtaa gcgactttat ttccccctgc
    48421 agaagcgatg gagtgcccgt gagatacata agcgccggtg ttagacagaa catccagact
    48481 ataacctttc aatgttccgt cgcggttcac gcccatttgc ccgtcaatag taaaagcgtg
    48541 gcgggtacgg gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc
    48601 gccaagcttg ctggtcagga atgccgccat tggctcttcc agtacatcct gtttattacc
    48661 aaaactgcca ccgacaaatg ttttgatgac tcgtacgcat gaccagggaa tatccagcgc
    48721 ctgaccaacc acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg
    48781 cgagtcatcc tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac
    48841 gggggtctga tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt
    48901 gcccgtcgac atcgtgcttt gtttcagtaa attgccgccg ttatggattg gtgcagcgtc
    48961 ctctgccagc gccgcttctg gcgtggtgat aacgggtaat tcttcccact caatgctgac
    49021 caattgcgcc gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc
    49081 accatgatga cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc
    49141 gtcaagtgtc catgcatgcc cagctgtagc gaatgggata tcaggcacat cttcccaggt
    49201 aaaaatcgcc agtacgcctg gtaaacttct ggcttgttca tcattaatac ttacggcata
    49261 accatgtgcg atagggctac gtacatattt cgcataacac atgcccgcca taacataatc
    49321 gtcagtatat cgtgcccgcc cggtgacctt agcaatggca tcaacgcgca tgcatgattc
    49381 acccgtagcg gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc
    49441 ttatggcaaa gatttagtct aagcaggcaa gatttacgcc agaaacggcg ataagcgtta
    49501 agggaggaag gaagtgtgat gatgttaaaa aaacacagat atgatacgaa acaggaccct
    49561 gaataggggt agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattagg
    49621 attattacgc atcaggatgg gaaatagggt aaaaaagtca ggaacttcga tacctctaca
    49681 gtgaatattt tgctgatgat acttcccttt gccaaatgaa attaacctct ttctctatta
    49741 catcgttttt ccagaagcaa gagattgggt atcaagaggc tggctgctat gatatggcgt
    49801 cttgtttttt aagcgaggaa agattttgag tgcgggacgc ctgaataaaa aatctctggg
    49861 tatcgtgatg ttgttatcgg ttggactgct tttggcgggc tgttcgggta gcaaatcatc
    49921 cgatacagga acgtattccg gctccgttta caccgtgaaa cggggggata cgctatatcg
    49981 tatttcgcgc accacgggaa ccagcgtaaa agagctggcg cgactgaacg gcatttcccc
    50041 cccttacacc attgaagttg gtcagaaact aaaactgggt ggggcgaaaa gtagcagtag
    50101 tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca tcggttacac cgtcatcagc
    50161 ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt tggttatggc caacgacagg
    50221 gaaagttatc atgccgtatt cgacagcaga tggcggcaat aaagggattg atatctcagc
    50281 tccacggggt acacctattt acgccgcggg tgcaggaaag gtggtgtatg tgggcaacca
    50341 gctgcgtggc tacggtaatc tcatcatgat taaacacagt gaagattaca ttacggctta
    50401 cgcccataat gacacgatgc tggtaaataa tgggcaaagc gtgaaggctg ggcaaaaaat
    50461 cgccaccatg gggagcacgg atgcggcatc tgttcgcctg catttccaga ttcgttaccg
    50521 tgcaacggca attgatccgc tacgttactt gccgcctcag ggcagcaagc caaaatgctg
    50581 atggcgaatt aatcagcagt cagcagcgcg gcacttgcta agaagagcgt aaggtttata
    50641 atgccttacg catctcgaag cgggcgtagt tcaatggtag aacgagagct tcccaagctc
    50701 tatacgaggg ttcgattccc ttcgcccgct ccaatttatc ttcacctcat caatctgcca
    50761 tttttgctgt atctgatttt ctatattatc ttaattcaat cgcttcagac ttttcgcaat
    50821 ctctgtaaca ctcccccatt tttttgtaaa taaaaagcgt ttacgcaatg caatcctttt
    50881 gtaacaaccc aggtaaaata ccttattttc aagttgttaa ataagtggtt atctacgctt
    50941 ttaacaaacg attgatggaa tattttatga aagggaaatc tgcattaact cttctgctcg
    51001 ctggtatatt ttcgtgcggc acatgccagg cgacaggggc agaagtgacg agtgaatctg
    51061 tatttaacat tcttaattcc acgggggcag caacagataa aagttattta tcgttaaatc
    51121 cagataagta tcctaattat cgtcttctga ttcactcagc caaattacaa aatgaaataa
    51181 agagccacta taccaaagac gaaattcaag gattgctaac attaacagaa aacacacgaa
    51241 agttaacgtt gacggagaaa ccctggggaa cgtttattct tgcttctact ttcgaagatg
    51301 acaaaacagc ggcagaaacc cactatgatg ctgtctggct gagggatagt ttatggggct
    51361 atatggcatt agtatcagac cagggaaata gtgtagccgc aaaaaaggtt ctgctcaccc
    51421 tgtgggatta tatgtctacg cccgaccaga ttaagcggat gcaggacatc attagcaacc
    51481 caaaacgact ggatggtatt cccgggcaaa tgaatgctgt gcatatttga cagcaactcc
    51541 cccgtgatgg cagatgtgca ggaagaaggc aagccgcagc tgtggaacca taaacaaaat
    51601 gatgcgttag gtctttatct tgatctcctt atacaagcaa tcaatactgg tactattaac
    51661 gctgaagatt ggcaaaaagg tgaccgacta aaatcggttg cgctgctgat tgcttatctg
    51721 gataaagcca atttctatgt tatggaagat tccggagcgt gggaggaaga tgcccgatta
    51781 aacacatctt ccgttgcact ggtgacctct ggactggaac gtttgtcaaa cctactctcg
    51841 aagaaagact ctgtttttgt ctctgattta ctacgcgaag ctaaagtcaa tgaactggat
    51901 gaaactcttt caacaacgag gttgaaccac ctcatagata aaggttatga aagaataacg
    51961 ttacagcttg atttaggggg agaatcccca ggttatctgg aaaaagataa acattacaga
    52021 gaagccgatg cggctttact taatgttatt tatcctgcaa atctttcaaa gataaacacc
    52081 agaagaaaag agcaagttct aaaaatagtt aaaaaactag caggtccgta tggtataaaa
    52141 agatacgaga aggataatta tcagtcggca aatttttggt tcaatgatat taaaaccgat
    52201 accgatcaaa attcgcatgc aaaaagagaa aagagcttca ttccatcaac agaagcagaa
    52261 tggttttttg actcatggta tgcaaaatca gcagcaattg tttataaaga atcaagaaaa
    52321 gaagagtatt tgaatgactc agtacaattt atgaatagat cattggccca aataacaggc
    52381 gagaatatga ttggtgcaaa tggcaggagc gtaccagaaa tggcacttcc cgaaagctat
    52441 aactatatcc acaagtcggg aactttgcat gaagcgccaa gccctatcat tccgctaaat
    52501 tggtcgaaag cttcaatgac tctcatgttg aaagagatgt ccaaccttat caatgatgag
    52561 ggaattaaat aattttcata atgaggcggt cctggattaa aaggaccgct caaagagaaa
    52621 tcgaaagggg aggtaaaagt gatcaataaa ttactgttag cttacctgat aggtctggtt
    52681 gtgacatcga ctttaatatt tatcttctcg gaagaaaaag taacatatcg gttgtttgct
    52741 gcaataatta caggtttaac ctgg
//
LOCUS       DAZFDC010000015        51390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFDC010000015 DAZFDC010000000
VERSION     DAZFDC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.015"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..322
                     /locus_tag="ACHJ48_001047"
     CDS             <1..322
                     /locus_tag="ACHJ48_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4706652.1"
                     /translation="KQHMDDHLFHLIRSNTMAPTPVYADYLNNTTDFRPDPEDIFKCG
                     QTLECGDPRKNIRQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSC
                     FQPH"
     gene            584..2206
                     /gene="yhiJ"
                     /locus_tag="ACHJ48_001048"
     CDS             584..2206
                     /gene="yhiJ"
                     /locus_tag="ACHJ48_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4706653.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVLEKVDDWQIERFQQSIQDDKISFTIQTDYSEKYSMLSGMRAHILRR
                     NNHYQFIVTINSKNYGCPLDNTDINWCGIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQVDTNVNTIISILNRRDIILESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINDNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDSKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHTIINSKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHTGRMFSMHNCQINPSA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2396..2509
                     /gene="yhiY"
                     /locus_tag="ACHJ48_001049"
     CDS             2396..2509
                     /gene="yhiY"
                     /locus_tag="ACHJ48_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO4706654.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2572..3639
                     /gene="yhiI"
                     /locus_tag="ACHJ48_001050"
     CDS             2572..3639
                     /gene="yhiI"
                     /locus_tag="ACHJ48_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4706655.1"
                     /translation="MDKSKRHLAWWGVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETRDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3636..6371
                     /gene="rbbA"
                     /locus_tag="ACHJ48_001051"
     CDS             3636..6371
                     /gene="rbbA"
                     /locus_tag="ACHJ48_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO4706656.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPLLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6371..7495
                     /gene="yhhJ"
                     /locus_tag="ACHJ48_001052"
     CDS             6371..7495
                     /gene="yhhJ"
                     /locus_tag="ACHJ48_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4706657.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLIMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7568..7843
                     /locus_tag="ACHJ48_001053"
     CDS             7568..7843
                     /locus_tag="ACHJ48_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO4706658.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            7840..8199
                     /locus_tag="ACHJ48_001054"
     CDS             7840..8199
                     /locus_tag="ACHJ48_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO4706659.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8249..9109)
                     /locus_tag="ACHJ48_001055"
     CDS             complement(8249..9109)
                     /locus_tag="ACHJ48_001055"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO4706660.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(9141..9410)
                     /locus_tag="ACHJ48_001056"
     CDS             complement(9141..9410)
                     /locus_tag="ACHJ48_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO4706661.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(9400..10908)
                     /locus_tag="ACHJ48_001057"
     CDS             complement(9400..10908)
                     /locus_tag="ACHJ48_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO4706662.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQHTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(10901..12259)
                     /locus_tag="ACHJ48_001058"
     CDS             complement(10901..12259)
                     /locus_tag="ACHJ48_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO4706663.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(12336..12617)
                     /locus_tag="ACHJ48_001059"
     CDS             complement(12336..12617)
                     /locus_tag="ACHJ48_001059"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4706664.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEQGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(12614..13087)
                     /locus_tag="ACHJ48_001060"
     CDS             complement(12614..13087)
                     /locus_tag="ACHJ48_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO4706665.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(13112..13858)
                     /locus_tag="ACHJ48_001061"
     CDS             complement(13112..13858)
                     /locus_tag="ACHJ48_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4706666.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LDVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(14057..14458)
                     /gene="nikR"
                     /locus_tag="ACHJ48_001062"
     CDS             complement(14057..14458)
                     /gene="nikR"
                     /locus_tag="ACHJ48_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO4706667.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(14464..15270)
                     /gene="nikE"
                     /locus_tag="ACHJ48_001063"
     CDS             complement(14464..15270)
                     /gene="nikE"
                     /locus_tag="ACHJ48_001063"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO4706668.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLSRLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(15267..16031)
                     /gene="nikD"
                     /locus_tag="ACHJ48_001064"
     CDS             complement(15267..16031)
                     /gene="nikD"
                     /locus_tag="ACHJ48_001064"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO4706669.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(16031..16864)
                     /gene="nikC"
                     /locus_tag="ACHJ48_001065"
     CDS             complement(16031..16864)
                     /gene="nikC"
                     /locus_tag="ACHJ48_001065"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO4706670.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(16861..17805)
                     /gene="nikB"
                     /locus_tag="ACHJ48_001066"
     CDS             complement(16861..17805)
                     /gene="nikB"
                     /locus_tag="ACHJ48_001066"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO4706671.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFATQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFTWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(17805..19379)
                     /gene="nikA"
                     /locus_tag="ACHJ48_001067"
     CDS             complement(17805..19379)
                     /gene="nikA"
                     /locus_tag="ACHJ48_001067"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4706672.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLHIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(19490..20077)
                     /gene="acpT"
                     /locus_tag="ACHJ48_001068"
     CDS             complement(19490..20077)
                     /gene="acpT"
                     /locus_tag="ACHJ48_001068"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO4706673.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(20079..21308)
                     /locus_tag="ACHJ48_001069"
     CDS             complement(20079..21308)
                     /locus_tag="ACHJ48_001069"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO4706674.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(21305..22036)
                     /locus_tag="ACHJ48_001070"
     CDS             complement(21305..22036)
                     /locus_tag="ACHJ48_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO4706675.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(22036..22500)
                     /locus_tag="ACHJ48_001071"
     CDS             complement(22036..22500)
                     /locus_tag="ACHJ48_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO4706676.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFTLELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(22497..23666)
                     /locus_tag="ACHJ48_001072"
     CDS             complement(22497..23666)
                     /locus_tag="ACHJ48_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO4706677.1"
                     /translation="MIYISAVGMINALGNNLDEIATNLTRGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLRTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGTAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(23668..24252)
                     /locus_tag="ACHJ48_001073"
     CDS             complement(23668..24252)
                     /locus_tag="ACHJ48_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO4706678.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRKGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(24249..26567)
                     /locus_tag="ACHJ48_001074"
     CDS             complement(24249..26567)
                     /locus_tag="ACHJ48_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO4706679.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAVALLILLPQSRLNSSVLAM
                     LPKQTMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPLVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPNTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSLGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVNVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(26536..27141)
                     /locus_tag="ACHJ48_001075"
     CDS             complement(26536..27141)
                     /locus_tag="ACHJ48_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO4706680.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(27138..27560)
                     /locus_tag="ACHJ48_001076"
     CDS             complement(27138..27560)
                     /locus_tag="ACHJ48_001076"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO4706681.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(27564..29240)
                     /locus_tag="ACHJ48_001077"
     CDS             complement(27564..29240)
                     /locus_tag="ACHJ48_001077"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO4706682.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAT
                     TQQQLDSLLAEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQQPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSCHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQPGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVN
                     PQRGGDWRVCWSSFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCESFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(29231..29584)
                     /locus_tag="ACHJ48_001078"
     CDS             complement(29231..29584)
                     /locus_tag="ACHJ48_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO4706683.1"
                     /translation="MKPHEIERHQAQTNHLEIVLHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(29571..30932)
                     /locus_tag="ACHJ48_001079"
     CDS             complement(29571..30932)
                     /locus_tag="ACHJ48_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HFO4706684.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLNESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQIATQSFTFAAIAPEAFIELFTSGSTGQPKRAIKPVHLLDREA
                     ELLAERLGARLAGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPLPGMQFQAEGDAFRLFSPLIAEDNGMLLDDILQFSEDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGGGHSQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(30929..31510)
                     /locus_tag="ACHJ48_001080"
     CDS             complement(30929..31510)
                     /locus_tag="ACHJ48_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706685.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLMALL
                     LLLRLRQTRRQAGPLQAVTQLVAVVGIALCVASFMLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGVRYTRHVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLLRRQVMKRDRA"
     gene            complement(31515..31766)
                     /locus_tag="ACHJ48_001081"
     CDS             complement(31515..31766)
                     /locus_tag="ACHJ48_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4706686.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPETRLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(31778..32035)
                     /locus_tag="ACHJ48_001082"
     CDS             complement(31778..32035)
                     /locus_tag="ACHJ48_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO4706687.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(32010..32831)
                     /locus_tag="ACHJ48_001083"
     CDS             complement(32010..32831)
                     /locus_tag="ACHJ48_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO4706688.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILHQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRATDYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(32828..33550)
                     /locus_tag="ACHJ48_001084"
     CDS             complement(32828..33550)
                     /locus_tag="ACHJ48_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO4706689.1"
                     /translation="MKFALNILDWQARAPGLSETLQWQEWSRQPQAIDPTAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPSVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDDWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNVDTFSLPGERVQWRWSHR"
     gene            complement(33591..34649)
                     /locus_tag="ACHJ48_001085"
     CDS             complement(33591..34649)
                     /locus_tag="ACHJ48_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4706690.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYATSVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGTFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(34718..35095)
                     /locus_tag="ACHJ48_001086"
     CDS             complement(34718..35095)
                     /locus_tag="ACHJ48_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4706691.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(35487..36536)
                     /gene="yhhT"
                     /locus_tag="ACHJ48_001087"
     CDS             complement(35487..36536)
                     /gene="yhhT"
                     /locus_tag="ACHJ48_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO4706692.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            36668..37885
                     /gene="yhhS"
                     /locus_tag="ACHJ48_001088"
     CDS             36668..37885
                     /gene="yhhS"
                     /locus_tag="ACHJ48_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4706693.1"
                     /translation="MPEPVAEPALNGLRLNLRILSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDVKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(37889..38446)
                     /gene="dcrB"
                     /locus_tag="ACHJ48_001089"
     CDS             complement(37889..38446)
                     /gene="dcrB"
                     /locus_tag="ACHJ48_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO4706694.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(38519..39184)
                     /gene="yhhQ"
                     /locus_tag="ACHJ48_001090"
     CDS             complement(38519..39184)
                     /gene="yhhQ"
                     /locus_tag="ACHJ48_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO4706695.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            39405..39650
                     /gene="tusA"
                     /locus_tag="ACHJ48_001091"
     CDS             39405..39650
                     /gene="tusA"
                     /locus_tag="ACHJ48_001091"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO4706696.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(39752..41950)
                     /gene="zntA"
                     /locus_tag="ACHJ48_001092"
     CDS             complement(39752..41950)
                     /gene="zntA"
                     /locus_tag="ACHJ48_001092"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO4706697.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFATSWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(42024..42650)
                     /gene="yhhN"
                     /locus_tag="ACHJ48_001093"
     CDS             complement(42024..42650)
                     /gene="yhhN"
                     /locus_tag="ACHJ48_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO4706698.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            42791..43150
                     /gene="yhhM"
                     /locus_tag="ACHJ48_001094"
     CDS             42791..43150
                     /gene="yhhM"
                     /locus_tag="ACHJ48_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO4706699.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(43153..43422)
                     /gene="yhhL"
                     /locus_tag="ACHJ48_001095"
     CDS             complement(43153..43422)
                     /gene="yhhL"
                     /locus_tag="ACHJ48_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO4706700.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(43412..44008)
                     /gene="rsmD"
                     /locus_tag="ACHJ48_001096"
     CDS             complement(43412..44008)
                     /gene="rsmD"
                     /locus_tag="ACHJ48_001096"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO4706701.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADETLIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            44158..45654
                     /gene="ftsY"
                     /locus_tag="ACHJ48_001097"
     CDS             44158..45654
                     /gene="ftsY"
                     /locus_tag="ACHJ48_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO4706702.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAENEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            45657..46325
                     /gene="ftsE"
                     /locus_tag="ACHJ48_001098"
     CDS             45657..46325
                     /gene="ftsE"
                     /locus_tag="ACHJ48_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO4706703.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            46318..47376
                     /gene="ftsX"
                     /locus_tag="ACHJ48_001099"
     CDS             46318..47376
                     /gene="ftsX"
                     /locus_tag="ACHJ48_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO4706704.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            47621..48475
                     /gene="rpoH"
                     /locus_tag="ACHJ48_001100"
     CDS             47621..48475
                     /gene="rpoH"
                     /locus_tag="ACHJ48_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO4706705.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            48746..49849
                     /gene="livJ"
                     /locus_tag="ACHJ48_001101"
     CDS             48746..49849
                     /gene="livJ"
                     /locus_tag="ACHJ48_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO4706706.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            49999..50265
                     /gene="ataR"
                     /locus_tag="ACHJ48_001102"
     CDS             49999..50265
                     /gene="ataR"
                     /locus_tag="ACHJ48_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFO4706707.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            50272..50799
                     /gene="ataT"
                     /locus_tag="ACHJ48_001103"
     CDS             50272..50799
                     /gene="ataT"
                     /locus_tag="ACHJ48_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFO4706708.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(50796..51179)
                     /gene="panM"
                     /locus_tag="ACHJ48_001104"
     CDS             complement(50796..51179)
                     /gene="panM"
                     /locus_tag="ACHJ48_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO4706709.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT        12348 a        13343 c        13830 g        11869 t
ORIGIN      
        1 taaacaacat atggatgatc atcttttcca tctaattaga agcaatacta tggcacccac
       61 acctgtatat gctgattatt taaataacac tactgatttc agaccagatc ctgaagatat
      121 cttcaaatgt ggacagacgc tcgaatgtgg cgacccccgc aaaaacatta gacaaaaata
      181 tggccatcat gggttagggg taaatcagaa tcaacaattt gataatggtg ttatggggct
      241 aaattccagg atggcagtgt ttgatgaaaa taaaaaaata ataggttctt cctcaggact
      301 aagttgtttt caaccacatt aaaactctat gagtgctgtg gtatatcttt ttaatcctgc
      361 cagtatttca tagtttttct taatcgattg tagaggcatg tactgatgac gcctctacaa
      421 tctttaggga ctttctctac tatgagataa gaaatttcat attatgcttt aaggctgaat
      481 gatttgtgca aaatataatt atttttccat cgaacatgag gtgtatgtca taagattaaa
      541 tgataactat gtattcctcg cggtgaatta tatgaggtaa aagatgaaaa ttggaactgt
      601 ggcaggtact aacgacagta caacgacaat cgcaacaaat gatatggtgc aagagcatgt
      661 tactaacttc actaaagaac tgtttgggta tattgcaaat ggtataggtg atgatatatc
      721 aagtattgcc aggacaatgc tcggtgaggt tctggaaaaa gtagatgatt ggcaaataga
      781 acggttccaa caatctattc aagatgacaa aatttcattt accatacaaa cggattattc
      841 agaaaaatat agtatgctat cgggcatgcg tgctcatatt ctacgtagga ataaccatta
      901 tcagtttatt gtgacgatta atagcaaaaa ttacggttgc cctcttgata ataccgatat
      961 taattggtgc ggcattgttt atttacttaa taatatgaca gtaaatgata atgctaatga
     1021 tgttgcagtt actgagtcgt acaaacccat ctggaactgg aaaatatcgc aatacaatgt
     1081 atctgatatt aaattcgaaa caattataaa accccagttc gctgacagaa tctatttctc
     1141 caattgttca cctgttgatc ccacaagtac cagaccaacc tattttggtg ataccgatgg
     1201 ctcagttggt gcagttttgt acgcgctttt tgcaacggga catttgggaa tcatggctga
     1261 aggagaaaat ttccttagcc agttgcttaa tatagaagat gaagttctca atgtattgct
     1321 aagggagaac tttaatgagc aagttgatac taatgttaat acaataatca gtatcctgaa
     1381 cagaagagac attattttag aaagtcttca gccttattta gttattaata aagacgcagt
     1441 gacgccatgt acgtttttgg gtgaccaaac cggcgatcga tttagtaata tctgtggcga
     1501 tcaatttatc atcgatttgt taaaacgcat aatgagcatc aatgataacg ttcatgtatt
     1561 agctggaaat catgaaacga attgcaatgg taactatatg cagaacttca cgcgtatgaa
     1621 accacttgat gaggatacct acgacgggat aaaagattat cctgtatgtt tctatgactc
     1681 caaatacaaa ataatggcaa atcatcatgg gataactttt gatgatcagc gaaaacgtta
     1741 cattatagga ccgataacgg tctctattga tgaaatgacc aatgctctcg atccggtcga
     1801 attagcggca attattaata aaaagcatca tactataatt aatagtaaaa agtttaaaac
     1861 tagccgggct atctcttgtc gatcctttaa tcgttatttt tctgtttcga cagattacag
     1921 acctaaactt gaagcactat tagcatgctc acaaatgtta ggtataaatc aagttgttgc
     1981 acataacggt aatggtggaa gagaatgcat aggtgaaacg gggacagttt taggtcttaa
     2041 cgcccgtgac agtaaacata cgggaagaat gttcagcatg cataattgcc aaattaaccc
     2101 tagtgcgggg cctgagataa ccactccctg gaagtcttac caacatgaaa aaaacagaaa
     2161 tggacttatg cctttgatta gaaggcgcac aatgttacag ctctgaacca tagacaacat
     2221 gaatattaat gttactatct ggttgagact gttgataaaa cgtaataagg aatgctctgt
     2281 tcctgcaact gaacaggcgt gaaacgctat ccaacaggat ggataccgtt ttgtcagcca
     2341 gttttataga tcacacttat gagtatgcgt gtttcattta cggccaggaa tgctgatgat
     2401 gacaacatta ctacccgttt tcactaagcc ttccccgttg gcgctcaatg ctctgcgcgc
     2461 tggtcgtatt tgtcgtttcc ttcttatccc ggatggaaga atccgctgaa cgtcgttcgg
     2521 cggcagttgt aacacgcgtc gtttgcttac agggattatg gcgggtcgat tatggataag
     2581 agtaagcgcc atctggcgtg gtggggtgtc gggttactgg cggtggctgc tatcgtagcg
     2641 tggtggctgt tgcgcccggc aggtgtgccg gaaggctttg ccgtcagcaa tgggcgcatt
     2701 gaagcgacgg aagtggatat cgccagcaaa attgccgggc gtatcgacac cattctggtg
     2761 aaagaaggcc agtttgttcg cgaaggcgaa gtgctggcga agatggatac tcgcgtgttg
     2821 caggagcagc gactggaagc catcgcgcaa atcaaagagg cacaaagcgc cgttgccgcc
     2881 gcgcaggctt tgctggagca acgacaaagc gaaactcgcg ccgcacagtc gctggttaat
     2941 caacgccagg ccgaactgga ctccgttgcc aaacgtcata cgcgttcccg ctcgctcgcc
     3001 caacgagggg ctatttctgc gcaacagctg gatgacgatc gcgccgccgc tgagagcgcc
     3061 cgagctgcgc tggaatcagc aaaagcccag gtttcggctt ctaaagccgc tatagaagcg
     3121 gcacgcacca atatcattca ggcgcaaacc cgcgtcgaag ccgcacaagc cactgaacgg
     3181 cgcattgccg cagatatcga tgacagcgaa ctgaaagccc cgcgtgacgg acgcgtgcag
     3241 tatcgggttg ccgagccagg ggaagtgctg gcggcaggcg gtcgggtgct gaatatggtc
     3301 gatctcagcg acgtctatat gaccttcttc ctgccaaccg aacaggcggg cacgctgaaa
     3361 cttggcggtg aagcccggct gatcctcgat gccgcgccag atctgcgtat tcctgcaacc
     3421 atcagttttg tcgccagtgt cgcccagttc acgccaaaaa ccgtcgaaac cagagatgaa
     3481 cggctgaaac tgatgttccg cgtcaaagcg cgtatcccac cggaattact ccagcagcat
     3541 ctggaatatg tcaaaacggg cttgccgggc gtagcgtggg tgcgggtgaa tgaagaactt
     3601 ccgtggcctg acgacctcgt ggtgaggttg ccgcaatgac gcatctggaa ctggttcccg
     3661 tcccgcctgt cgcgcaactg gcgggcgtga gccagcatta tggaaaaacc gttgcgctga
     3721 acaatatcac actcgatatt ccggcccgct gtatggtcgg gctgattggt ccggatggcg
     3781 tcgggaagtc gagtttattg tcattgattt ccggtgctcg tgtcattgag cagggcaatg
     3841 tgatcgtgct gggcggcgat atgcgcgacc cgaagcatcg ccgcgacgtc tgcccgcgca
     3901 tcgcctggat gccgcagggg ctgggcaaaa acctctacca caccttgtcg gtgtatgaaa
     3961 acgtcgattt tttcgctcgc ctgttcggtc acgacaaagc ggagcgggaa gtgcgaatca
     4021 atgagctgct gaccagcacc gggttagcac cgtttcgcga tcgtccggca gggaaactct
     4081 ccggcgggat gaagcaaaaa ctggggctgt gctgtgcgtt aatccacgac ccggaactgt
     4141 tgattcttga tgagcctacg acgggggttg acccgctctc ccgcgcccag ttctgggatc
     4201 tgatcgacag tattcgccag cggcagagca atatgagcgt tctggtcgcc acggcctata
     4261 tggaagaggc ggaacgcttc gactggttgg tggcgatgaa tgccggagaa gtgttggcaa
     4321 ctggcagcgc cgaagagctg cggcagcaaa cgcaaagcgc cacgctggaa gaagcattta
     4381 taaatctgtt accgcaagcg caacgccagg cgcatcaggc ggtagtgatc ccaccatatc
     4441 aacctgaaaa cgcagagatt gccatcgaag cgcgcgatct gaccatgcgt tttggttcct
     4501 tcgttgccgt tgatcacgtc aatttccgca ttccacgcgg ggagattttt ggttttcttg
     4561 gttcgaacgg ctgcggtaaa tccaccacca tgaaaatgct taccggattg ctgcccgcca
     4621 gcgaaggtga ggcgtggctg ttcgggcaac cggttgatcc aaaagatatc gatactcgcc
     4681 gtcgggtggg ctatatgtcg caggcgtttt cgctctataa cgagctcacc gtgcggcaaa
     4741 accttgagtt acatgcccgt ttgtttcaca tcccggaagc ggaaattccc gcaagagtgg
     4801 ctgaaatgag cgagcgtttt aagctcaacg acgttgaaga tgttctgccg gagtcattgc
     4861 cgctcggcat tcgccagcgg ctttcgctgg cggtggcggt gattcatcgc ccggaaatgt
     4921 taatcctcga tgaacctact tctggcgtcg atccggtggc gagggatatg ttctggcagt
     4981 tgatggtcga tctctcgcgc caggacaaag tgactatctt catctccacc cactttatga
     5041 acgaagcgga acgctgcgac cgtatctctc tgatgcacgc cggaaaagtg ctcgccagcg
     5101 gtacaccgca ggaactggtt gagaaacgcg gagccgccag tctggaagag gcatttatcg
     5161 cctatttgca ggaagcggca ggacagagca acgaagccga agcgcctccc gtgatacacg
     5221 acaccacaca cgcgccgcgc cagggattta gcctgcgccg tctgtttagc tacagccgcc
     5281 gcgaagcgct ggaactgcga cgcgatccgg tacgttcgac gctggcgctg atgggaacag
     5341 tgatcctgat gctgataatg ggttacggca tcagtatgga tgtggaaaac ctgcgctttg
     5401 cggtgcttga ccgcgaccag accgtcagta gccaggcgtg gacgctcaat ctctccggtt
     5461 cccgttactt tatcgaacag ccgctgctta ccagttatga cgagcttgat cgtcggatgc
     5521 gtgcgggcga tatcacggtg gcaattgaga tcccgcccaa tttcgggcgc gatatcgcgc
     5581 gtggtacgcc tgtggaactc ggtgtctgga tcgacggtgc gatgccgagc cgcgccgaaa
     5641 ctgtaaaagg ttacgtgcag gccatgcacc agagctggct acaggatgtg gcgagccgac
     5701 aatccacacc ctccagccaa agcgggctga tgaatattga gacgcgttat cgctataacc
     5761 cggacgtaaa aagcctgcca gcgattgttc cggcggtgat cccgcttctg ctgatgatga
     5821 tcccgtcaat gctaagcgcc cttagcgtgg tgcgggaaaa agagctaggg tcgattatca
     5881 acctttacgt gacccccacc acccgtagcg aatttttgct tggcaaacag ctgccgtaca
     5941 tcgcgctggg gatgctgaac tttttcctgc tctgcggcct gtcggtgttt gtgtttggcg
     6001 taccgcataa aggcagtttc ctgacgctca ccctggcggc gctgctgtat atcatcatcg
     6061 ccaccggaat ggggctgctg atctccacct ttatgaaaag ccagattgcc gccattttcg
     6121 gtacggcgat tatcacgttg atcccggcga cacagttttc cgggatgatc gatccggtag
     6181 cttcgctgga agggccggga cgttggatcg gcgaggttta cccgaccagt cattttctga
     6241 ctatcgcccg cgggacgttc tcgaaagcgc tggatctgac tgatttgtgg caacttttta
     6301 tcccgttgct gatagccata ccgctggtga tgggcttaag catcctgctg ctgaaaaaac
     6361 aggagggatg atgcgccatt tacgcaatat ttttaatctg ggtatcaaag agttgcgcag
     6421 tctactcggt gataaagcga tgctgacatt gattgtcttc tcgtttacgg tgtcggtgta
     6481 ttcgtcagcg accgtcacgc caggatcgtt gaacctggca ccgatcgcca ttgccgatat
     6541 ggatcaatcg cagttatcga accggatcgt taacagcttc tatcgcccgt ggtttttgcc
     6601 accggagatg atcaccgccg atgagatgga tgccggactg gacgccgggc gttatacctt
     6661 tgcgataaat attccgccta attttcagcg tgatgtcctc gccggacgcc agccagatat
     6721 tcaggtgaac gtcgatgcca cgcgcatgag ccaggcattt accggcaatg ggtatatcca
     6781 gaatattatc aacggtgaag tgaacagctt tgtcgcgcgc taccgtgata acagcgaacc
     6841 gttggtatcg ctggaaaccc ggatgcgctt taacccgaac cttgatcccg cgtggtttgg
     6901 cggggtgatg gcgatcatca acaacattac catgctggcg attgtattga ccggatcggc
     6961 gctgatccgc gagcgtgaac acggcacggt ggaacactta ctggtgatgc cgataacgcc
     7021 gtttgagatc atgatggcga agatctggtc gatggggttg gtggtgctgg tggtatcggg
     7081 attatcactg gtgctgatgg taaaaggcgt gttgggcgta ccgattgaag gctcgatccc
     7141 gctgtttatg ctgggcgtgg cgcttagtct gtttgccacc acgtcaatcg gcatttttat
     7201 ggggacaata gcgcgttcaa tgccgcaact ggggctgctg gtgattctgg tgctgctgcc
     7261 gctgcaaatg ctttccggtg gttccacgcc gcgcgaaagt atgccgcaga tggtgcagga
     7321 cattatgctg atcatgccga cgacacactt tgttagcctc gcgcaggcca ttctctaccg
     7381 tggtgccgga ttcgaaatcg tctggccgca gttcctgacg ctgatggcaa ttggcggcgc
     7441 gtttttcacc attgcgctgc tgcgattcag gaagacgatt gggacaatgg cgtaaggaag
     7501 ggaaacttga cttcatactg tgatgtcata ctattgatgt cataaagtga agtcataagg
     7561 acatgccatg agaaccaagg tacaggcttt acggaagaaa caaaaaaata cattggatca
     7621 aatatttaaa actcctgttc ctcaagggat caagtggtct gatatagagt cactggttaa
     7681 agcattaggc ggagaaatta aggaaggaag aggttcgcgt tgtaagctca tactaaatat
     7741 gagcgttgcg tgtttccatc ggcctcatcc gtcgccagat accgataaag gcgctgtaga
     7801 aagcgtgcgt gactggttac taagtatagg ggtaaaacca tgatcaaact gaagacgcca
     7861 aactctatgg agattgccgg gcaacctgct gttatcactt acgtgccaga actgaatgca
     7921 tttcgtggca aattcctggg cttgtcaggt tattgtgatt ttgtctctga cagcattcag
     7981 gggctgcaaa aagaagggga actttccctg cgagagtatc ttgaggattg taaagctgcg
     8041 ggtattgagc cgtatgcgcg tacagaaaag attaagacct ttacgttacg ctatccagaa
     8101 tcgctgagtg aacgccttaa taatgcggct gcgcagcaac aagtctctgt taatacctat
     8161 attattgaaa cactgaatga gcgtttgaat catttataac ttatagcggc ccaacaattt
     8221 ctgttgcgcc gctatatttt tgtggctctt aagatgcctt tctaaccaac cgatgcccct
     8281 cattcatcat caggattttg ctatacacat cttcctcgac ggcgtttttt gctgatgcca
     8341 tgactcgtgc cagattcgcc tcgttgtgat tattcacata cgctttgccg acggcggtga
     8401 taaacgcttt gcgcagatcg ctggcgatat ttactttagc gacgcgataa ttaacaaagc
     8461 tgcgtaatat ctccggggcg ataccggagc cgccgtgaat aaccaacggc accggacaga
     8521 cttcggcaat gcgcttcagc agggggatat cgatacgcgg tatatcgtcc aggccatgaa
     8581 cgttgccaat agaaactgcc agcatgtcgc aaccggtacg ctcgacaaag gtcttcacct
     8641 tttccggttc cgttttgcaa tcggcttcgc tgacgtgatc atcttctttg ccgagaatcg
     8701 cccccagctc cgcttcaacc ggtacgccat aggatttaca gaaatcgaca gcttcctggg
     8761 tgaaagcgat attctcttca aaagagaatg cggcaccgtc aatcatgacc gaggtaaagc
     8821 ccgcgcgcac cgcctgttta acatcctcct gggttttccc gtgatccaga tgaagactga
     8881 ccggaacatc cattttatcg gcgtgacgtt tcacgatttc ataaatgtaa tcgtatcccg
     8941 agagttgtgc attggttggc gcaatctgaa tgaagttggg taatcccgaa cgctcaatag
     9001 cgtttaaaat tgaaattgtc gtctccagat tggtggtatt aaatgcgccc gctaataccc
     9061 gtttttcctg aatgcggtcg agcagtatcc gtccattaac taatggcata ttgaaaacct
     9121 catcttttaa tatcagttaa ttattcagct tcaccgaagc ggtcgcgaaa taagtcggtg
     9181 agagtctgta tggcctcgtt ttcatcatca ccgtcgcagc gtatttccac cgtttttccg
     9241 ccttttattc cggcgcgcat aatattcatc atgctggtgg cattaatatc attaccctca
     9301 tagtgcagcg tcactttaca gctatatttt ttggcggctt tcgccagaca ggccgccgga
     9361 cgtgcatgca ggccagtact gtttctgacc tccacggttt tattcaacat agtgttcctc
     9421 tcgatgatgt aatggcatca cgccttcttg ttgtaatttc aactgatacc agcttttcca
     9481 tttgtgctga taacgctgta attgatggta tctcgcccga ttcggtaaca aacgttgggc
     9541 tatagcgact ggcggcggtg gaatatccgg tgcggcggtt aataatgctc cgacagaaac
     9601 cgcctcgtta aatgcaattg cttcaaccgg acgctgtaaa atatccgcct ttaattgcag
     9661 ccaaagagga ttatgggttg ccggaccgat cacttttaac gcgctggtgc caggcacgtt
     9721 aaaacaggcg aagcagttgg caaattccat ggttaatccc agtgatacgc tggcaattaa
     9781 catgtcgata cttaacgtat cgccaagccc ataaaataat ccacgggtat ggcgattttt
     9841 atagggcgaa cccgaaccgc gaagatgtgg aataaagacg ggaatgtcat cgagcaacca
     9901 attccccgcc agataatcag catgtccgcg agtaagtgat gcggcgatct cctcatcggt
     9961 tagccggaac gtgttgcgca gccactccag cgcgaaaccg cccacgggca gcgacgcaaa
    10021 cagggtgaac tggttggcaa gtgagtaaca gccgtttgcc agcttgtcac gtttggcctg
    10081 ttcatccagc gtcggttgtg tatccagttg cagaatgcct tccgtggtcc ccgtcgagtt
    10141 aaggatatcg cctggcatca tctgcaacgc tcgcgcaccc accatatggt catgtccggc
    10201 cagcgtcacg ctgaccggtt gcgagaaccc aagcgtcttg caaagtgact cgctgaccca
    10261 tccggcgtgc tcgcctggct gcaccaatgg cgcaaatgcc gtacagggaa cgtgtaacaa
    10321 cgccgccgct ttcgctgacc attcgccttt gcgcacgtcc agacacagcg tgcggctggc
    10381 taaggtgata tccgtgtgct gttcgccagt cagccgccag agcagcactt ccggcgcatg
    10441 tagccagcgc aggccgcggc gattacgcag cgggtaatgt tccagcaacc agcgcatttt
    10501 gaaggcagag taattgctgt gtagtggtag accgcagatg tcataaagtg ccgcactgtc
    10561 tgcctcgcta agcgttgcca gatactcttc accgcgacgg tcataccatg ccagcattgg
    10621 cgtcaggatc tcgccatgct cgtcaaggaa cacgcctgat tcgccaaaac tggcaatgct
    10681 gatccgtctg actggcagcg gcgaagcggc gttcagttgc gctatcgcct ggcggacctc
    10741 ctgccagagg gcgtcgatat cgaaatcgac atcgccctgt ggggagatct gctttgccgt
    10801 cacgaattta tgcgcgccca acgtcgttgc gtccaggcag gaaaagcagg tgactttgca
    10861 attggtggtg ccaatatcga taacgatagc tgcgctgtta tcaggcataa accgtgctcc
    10921 gcgtcattcg gaaaatgcca tacagtacca gggcgataac cggcaccaga ccaatggcga
    10981 tgagattgcc gttgaagatt tccagcagcc agaagcggaa cgggttaccg ccatcaagaa
    11041 aactggaaac ctggccgctg gagccttcgg caaatttgaa gttaacgtct ttcgccattc
    11101 cggtaataaa cggcgcgatt ttggtagcaa tccataggtc ggcaataatc accgggatcg
    11161 ccgccagaac ggtgcggaaa atattgccac ggctggctaa agcaatcatc gacgccatta
    11221 ccgccaggtt ggcaaggtcg ccaagcggca ggacttcatt acccggaagg acaaacgcga
    11281 taaagaccga gattggcgtt agcagcaggg cggtggagat aatcgccgga ttacctacgg
    11341 caacggcgat atccaggccg atatagagat cgtcacggtc agggtatttg gcattgagat
    11401 actttttaat agcttcagac agcggtaata aaccttccat caggatgcgt accatgcgtg
    11461 gcaggatgaa catcacaccg ccaatgctga tgccaagcag caatactttt ttgaaatcgt
    11521 atccggcaat tacgccgagc agaatgccca gaatagtgcc aaccatcatc ggttcgccga
    11581 agatgccaaa ctttttctgt acggtttccg ggtcgatatg aatacgattg aggccaggga
    11641 tatggtcgat gattttgtcg accagcagac cgaccgggaa gaacaccacc gaagagagcg
    11701 tcggcaatga gatcccttcc agtccaaagt atttttgtac cagtggcgca gaccagtcgg
    11761 cgagtttaag tactaccacc gcagcaatcg ccgccgccag caaaccaaag aacaggctgc
    11821 cggtgctgta ataaaccaac gtaccagcaa gggcaaaatg ccagtagttc cacatatcta
    11881 cgtcaacggt acgggtttta tttaacgcca gcatcgccac gttaatcaga atggtcaggg
    11941 gaataacaaa cggggcaatt ggcgaacccc atgtaatagc ggaaagcggc ggccagccta
    12001 aatctaccac cggtaaatta attccggtgc gttcaaccat cgcctgggcc gctggcccga
    12061 cattgctggt cagaatatcg aaaatggcat aaataccgac aaaaccaatg ccgactgtta
    12121 atgacgaacg taaggcttta gcgggtttta ctttaaaaca gagagcaaga ataaataaca
    12181 ccaacggcaa cattaccgtc gggccaaatc ccaatatata ttgcacaata ttatagaggg
    12241 tatgcgcgat atcgttcata ataaacctct ttttcaggcg agagcgcacc tcgcgttagc
    12301 gcctgatgga gtgggttgaa attaattatt attaattact ttttaaggat agaaacaatt
    12361 tgcgccagta ctttttcttc gccaataccg gtaataatcg gcaagccgtt caccaccggg
    12421 acgtcgagtt cataaggcac tttagttgtt gaaactacca gtgctacgcc gtcagtattt
    12481 ccaggtaaag aggcaacgtt ggtttgggaa taattgacgt taagaccttg ctctttgcaa
    12541 tattccatga ctttttctgt taccgccgtg gatgtggcaa tacccgtggc gcagacaaat
    12601 aaaatggttt gactcatatt tcacctcgtt ctttcatttt gtttaagatt actgtgtgta
    12661 tttctgttgt ttccatcgca agcagggctt gcataaaggg cgtatcctga atcatttcca
    12721 taatccagcc cagggcattt aattgtttat tgctttcgct taaggcgagc aaaaaaatca
    12781 ccctgacagg tacggggtca ggatcgccgc ccatatcggc aaactctacc ggttccggca
    12841 ggatgccgac ggcaattgca ttgtgcctga catgtttatg gtccgtgtgc ggaatagcga
    12901 cgcacggtgg ttcggtaggc agccccgttg ggtacgtcgc ctcacgtttc agtacgtgat
    12961 cggcaaaatc agggacgacc agctcggcgg caaccaacgg ttgcgctaac tggcggatga
    13021 tctgttcaac gcttaaccct gccggaagtc gttgaatata attttcctga aaatgtatgc
    13081 cttgcatttt attcctctta aaaaagtgta cttaactcac ttcccgccgt tgcagtacgg
    13141 tgccaagata atatttattt ccttttaacc agacgttgcc aaactcaatg ggtaatcctc
    13201 gggagaaaaa gactaactgc tccagatgca aaacgggtgc gtcttcactg atatctaaaa
    13261 aatgaccacg ttcattacca attaatcgcg cggcatagcg actttccgag taacgaattt
    13321 ttctttgcga caaactttct attgttggaa ataaattgtg ttgattaaaa tcgatttcag
    13381 caataccggg gcatagctca atattgatac ggttctctat cagcatcgct ttttcatcac
    13441 caattgagcg taagcgttca aggtaaagaa tatcctgacc gggcgttatt cttaatttct
    13501 ctgccacata acgattagcc gattcaatcc gtgacgtaat aacttcagtg gtaaaatgta
    13561 ttttctggct ttccagcgat tccgcgaatg acaataatcc ttcacctaac ggatatgcca
    13621 cgttctcttt tttgacaaat gttcccttac cctggacctg tattaacacg ccttctttta
    13681 ccagttgggc gaccgctttt ttaacggttc cccgactcac gtccagcata tccataatct
    13741 ggtattccga aggaatgcga tcgtcttcca ccagatctcc ggtataaata ctttctctta
    13801 tccactccac cacttgttga tagagcggta tttgtgacga cttacttaat gatttcatgg
    13861 cgcgcacctg aacatttaca gttgtctatg gacaatcatg gacaacttca agcgtatagt
    13921 aggtgaacgg gagaaaaaga ggatcgaagc gtctcacatt ttgagggacc aaatcgtgat
    13981 caaaaaaatg tgacgtgagt cgatatttgt cagaagttta ctgcgccgta aggaaataac
    14041 ggcgcgttgg taggtgttaa tcttccttcg gcaggcactg caaatgcccg tgccgcacgc
    14101 cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc acccatgtca cctttcaaca
    14161 cggcgatttc cagacagtcg tcgtggttga tgtgcacatg tagcgtggcg acggagaggt
    14221 cgtggtgatg atgctgggtg gaaacaatac ggctggctaa gtcgcgtttt tcgtgttcat
    14281 acacatacga cagcaccgcg aaaccttgtg tgccgtgctg ctgggtagct tcctgcgcca
    14341 gggcgctacg cagaatatcg cggatagctt cggaacggtt gttataacca cgacgctggc
    14401 tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt gatggtgact cgttgcattt
    14461 gcgttaaacc ttttctgtgg cgcgacggcg cacggggaat gcgggtaata tcgcgttttg
    14521 tagcacacgt ccggcgtcag aggaaaaggt taatttctct cccaccgcct gggtttcgac
    14581 gatttgtccg ttgtccatca ccatcactcg ctggcaaaag cgttccacca ggcgtaagtc
    14641 atgagtgatg aacaggcagg cagtgccaaa ctgttgttgt agctttttca gcaggcgaat
    14701 gacgcccgcc tgtaacacga gatcaaggtt agaaacggct tcatccagaa tcagcaattt
    14761 cggttcgacc gccagcgcgc gtgccaggca gacgcgctgg agctggccgc cgcttaactg
    14821 cggtgggcgc ttgtcgagca cgctgtcgtc gagatcgacc gcgtgcagca tttcgctggc
    14881 acgcgccagt tgttcggatt ttttcagcga tagcaggtgg cgcatcggtt cccgcaggat
    14941 ctcgcgcacg gttttgcgcg gattcacggc gctgatggag tcctgaaata ccatctgaat
    15001 atcgcggcgg aacgctttac gctgggcgcg attgagcttt gccaacgatt cgccacgcca
    15061 gctaatattt ccctgtgagg gcgattctaa gcctaccagt agccgcgaga gagtgctttt
    15121 tccgcagccg ctgcgtccca gcagggcgac ggtttcaccg cttttcaggg tcagggaaac
    15181 gttattcagc accgcctggt gctgatgttt tccgctaaac ccaccgtggg catagtgatg
    15241 ggaaaggtcg gagacgttaa gtaaagtcat gatgccagct ccataccgta gagggcgaga
    15301 tgagcggaaa ccaggctgcg cgttaccgca tgtttggggg cgttaaacag cgtttcgaca
    15361 tcgccctgtt cgacaatctt accgtcagac attaccgcta cgtcatccgc cagacgcgcc
    15421 accacgccca tatcatgggt gaccagcagc atccccggcg cctgtttttg cataatgctt
    15481 tccagcagat cgagaatgcg tgcctgtgct accacgtcga ggtcggtggt tggttcatcg
    15541 gcgatgataa acggtgattc gcaaagcacc gccatcgcaa tcatcatgcg ctgcaacatg
    15601 ccgccgctca tctcgaacgg gtacagcttc agcacgcgtg cggcgttttc cagccccacc
    15661 gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt tccccagcgc caggcacgtc
    15721 tcccgcgcgt gggtgtgcat ggtgtgcagt ggattaaagg cgctgcgcgg gttctgcatg
    15781 atggtggcaa ttttgatgcc gcgcagggcg cagggagaaa ccggtttgcc atcggctaaa
    15841 atttcccccg ccgtctggcg aacgcctgcg ggcagaattc ccagcgtcgc ggcgcaggtt
    15901 aatgatttcc cgctgccgct accgccgact aacgccagca cgcgcccgcg tttcagggtt
    15961 aacgacacgc cgtgcaccag cggctgcgcg gcctgtagcg cgatattacg tagttcaatc
    16021 tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg atccagatga tcgcgcagtg
    16081 cgtcacccac caggttaaag gccatcacgc tgataaacag cgccagcccc ggccagaaca
    16141 tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt aatcatcacg ccccattcgg
    16201 cggtcggcgc ggtcacgccg aggccgagga aggacatccc ggcaacgtgc agcatcatat
    16261 ggccgatatc cagcgttgcc agcaccagca gcgaagggat caccgcacct gccagatgat
    16321 caacaaacac tcgcacatgg cccgcgccag aaagccgtga tgccagcaca aactcgcgct
    16381 ggcgcagtga aatcaccagg ctgcgcacca tgcgtgcata ccacgcccag tgcgacaggg
    16441 cgatggcgat aattacgttg gtcagcccag tgccgagcac gccaaccata aagaatgaca
    16501 gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg catggtagcc tgatcaacgc
    16561 gcccgccaat cagcccggcg ctgccgccaa taaccagccc taatgtcagc accagcagca
    16621 ggcaggccat taccgaaccg agcgacacac gggtcgctgc catcagccgc gagaaaatat
    16681 cgcgccctaa gtgatcggtg cccaaccagt gctgcgcatc cggcgaaagc aggcgcgacg
    16741 gcaaatcaat cgcctggggg tcatacggca gccaccactg gctggtgagc gcaatcagcg
    16801 ccagcagggc gataatgatc agcgccaggc gtaccgacca gcgggaagag aggaaaaagt
    16861 tcacgcgtgc gctccttcat gacgacgaat gcgtgggtcc agtgcggcgt tgagcaaatc
    16921 gacaatcaaa ttacagacca caaaaaccac caccatcatc agcgtaaagc actggatcac
    16981 cggatagtca cggttaaaaa tcgccgacac cgcatagcgc ccgacgccgg gccaggtaaa
    17041 gatgttttcg ataatcatcg tcccgccaat cagttcgcca atgtgcatcc ccacggcggt
    17101 gatcatcggc agcgaggcat tgcgcagaat atgacgacgc tcggtctgtt tgtcattcag
    17161 gccgcgtagc cgcgcccagg tgacgtgacg ctgaccggcg acgtccagca tactggcgcg
    17221 cagcagccgt gcgttaatcg ccagcgacat aaaggcaatg gaaaccgcag gcaaaatgat
    17281 gtgctgccag ccgccgtagc ccatcgcggg tagccattgc agatacaccg aaaacgccat
    17341 caccagtaaa aatgccagcc agaagttggg catcgacacg ccgagaaacg cgatgaaacg
    17401 tacggcgaaa tccggcagac ggtcacgatg gcgcgccgcc cagataccga gcggtacgga
    17461 agtgagcaga attaatacca gcgccgcacc cgccagttcc agcgtggcgg gcaggaagtt
    17521 cagcatatcg tccagcaccg ggcgctgcgt ggcaaatgag atgccgaaat caagatgcag
    17581 cgccttccac aaccaggtgc cgtactgcac atacagcggc tgatccagtc ccagcatggt
    17641 gcgggtagag gccagcatct ccggcgtcgg cggcaggtta gacagacgca aataatcgag
    17701 cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg atcaccgagg cggcgagcac
    17761 catcgggatc agcagcagaa agcggcgtaa tacgtaacgc aacattaagg tttcaccggt
    17821 ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt aggggatgtt acccagctcc
    17881 ggttttgata ccaccatcat tgagatgtaa ctgataggca gataaaccgc ctcgtcatgc
    17941 agacgggtca gaatgtcgcg atacagtgcc tgacgttgtg cttcgtcatg ggtcgccagc
    18001 acttcgccga tctctttatc aatcagcggc ttgtcggcta atccttgctg tgcctggaag
    18061 tcagcgtgcg acggtacgcg cattgaactc aggaaggcgt gtggatcgta tggcgcgccc
    18121 caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct gacgcgcata gatactgctc
    18181 tcttcttcgc caatcagcga gacatctgcg ccaatctggc gcatatcagc ctgaatgatt
    18241 tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg aaagttcaat gtgcagcggc
    18301 tgaccatttt tctcgcggat gtctttgccc gcaggcagcg tccaaccggc tttttccagc
    18361 aacgctttcg ctttctgcgg atcgtactgg cgcggtttca ggccgaggtt ggcgtagggc
    18421 acagaagggg caaacagggt gtcggcgacc tgctgggtgc catacaacgc gttatcaatc
    18481 agcgattttt tgtttaccgc gtaattaaga gcttcacgta ctgccagctc attggtgggg
    18541 gctttggcgg tattaagcgc cagcatcacg gtttcgatcg gctgtgacag ttgggtatgg
    18601 taagccgggt tctggctaaa gcgggcgaag gtatcgagcg gtaataaccc ttcgtttccg
    18661 tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc gggtagtcgg gtctgggatg
    18721 acgttaaaag tgatcttctt aattgccggc ttttcgcccc agtagttttc gttacggacg
    18781 aagacatcgt actgattcag tttcgattcc tgcaaaaccc acggtccggt gccaatcggc
    18841 gctttaatcc cgttcatggt ttcatggttt ttaaactgcg agggggcgat aaagcggaaa
    18901 gggcgcggca gggccagttc ttgcaggaaa ggatagtagg cgcttttcag ggtgatttgc
    18961 agctctgttt tactgagtgc tttaacatca acaatctggt ttgccagctc cagccaggcg
    19021 tgacgttgac ggttatcgag cactgcgcgg aagttttctg ccgccgcctc tgcatcaaac
    19081 ggttcgccgt tagagaattt cacgtcatca cgcagggtga aggtccaggt tttaccatct
    19141 tctgaatgag tccagctttt tgccagccac gggatcaccg aaccgtctgc ctgatatttc
    19201 accaatggtt cataaaccat gctctgggcg aacatctggt taggcgtgta aaggtgcggg
    19261 tttagtggcc cgacattcac cggccaggcg gtggtgattt catctggtgc agcggcatgg
    19321 acgataaaag acgcacaagc cagcagcgca aatagagtgc ggcggagtgt ggagagcatg
    19381 gtaaccccaa tggattaaaa tagatggcgg aaataagtat gacgatttta agtattcgtc
    19441 atactgatta cctgttctgg atcaatagat gggcagtcgg gtgggcggat cagttaactg
    19501 aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg agtaggggtg cagatcgcca
    19561 ggcttaaatt ttctagctga caatggctga ctgacagcga ggagtgatag gtgctgtcta
    19621 cgctgacgat ttgccaggcg ctgccaccac gctgtttaac gatggcttct ttgcgcgtcc
    19681 agatgcgcca gaacatttcc agttgctgat caggatgcac ggcgtccatc tcagcatgtt
    19741 ccccgaggct gaacacggcg ttcgccagcc agcgccagtt ggcgcgcggg cgaatcactt
    19801 cgatatcgca gccgacttcg ccttcatcac tcaacagcag ggcgatatcg tcaccgctat
    19861 ggcttaagtt gaaccatagc ggcgtttccg gcgcaaatgc aggtttgcct tgttcgccat
    19921 agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa tgcacgcccc gccagccagc
    19981 gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc cggtggcagt ggagctgcgc
    20041 ttaaggtcga aactttcccc agaactatcc gatacatatc agggccaacg tttaatgatg
    20101 atggatgtat taatgccgcc aaaggcaaag ttgttactct gcaaaaactc acaatcaacc
    20161 ttgcgggctt catgcatgat gtaatccagc gcgccacaat tcgggtctgg tttattcagg
    20221 tttagcgtag gcgcaaacca accttcgcgc atcatttgca gactcatcca ggcttcgagc
    20281 gcgccacagg caccaagcgt atgaccaaaa taacttttca gcgaggagag cggcacatta
    20341 tcaccgtaaa tggttgctgt cgccagactt tctgccatat caccgcgatc ggtcgccgta
    20401 ccatgcgctg aaatatagcc gatatcttgt gcgcttaacc cggcgatttt cagcgattgc
    20461 tccatacaat actgcatggt ttcgcgctgc ggctgggtaa tatgtgccgc gtcgcagtta
    20521 gtcgcaaagc cgacgatttc accgtaaatg gtcgcgccgc gcgctttggc gtgctccagt
    20581 tcttcgagaa tgagcgtacc tgcgccttca ccaatcacca gcccgtcgcg gttttcatcg
    20641 aacggtgaag gtgtggtttt cggcgcatca ttgtgctggc tggtggcgaa aagcgtatca
    20701 aataccgctg cttctgaagg acataactct tcggcaccac cagcaaccat caccgtttga
    20761 taaccgtggc gaatggcttc ccacgcataa ccgattgcct ggctgccgga agtacaggcg
    20821 ctggaggttg ggatcacccg cccgcgcagg ccgaaaaaga ggccagtatt aacggcggtg
    20881 gtgtgcggca tcatctgcac ataggtggtg ccagtgatgt tattggtgtg cttttccgtc
    20941 agcatggtgg cgaactcgct taccgggccg gtactgccgg tcgatgaacc gtacgcaatg
    21001 cccgtttcgc cgctggttaa tatcggatcg ccaatcaacc ctgcctgctc taacgccagc
    21061 tcgctggcac gggtcgacat ttgcgacacg cggcccatcg cacggatacg cttgcgggtg
    21121 tagtgctccg gtagcgtgaa atcatcaaca ggcgcgccaa gcagggtgtg cagcccatcg
    21181 tagacctgcc actccggcat tttgcgcacc gcattttcat atgccagtaa gcgtgcagaa
    21241 acgtcctgcc agttttcacc aaaggcagtc acgccgccca ttcctgtaat cactacgcga
    21301 cgtgtcataa catccctccg ttgatggaaa taacctggcg ggtgacgtaa cccgcaatat
    21361 cagacattaa atagctggca agcccggcaa cttcttccgc ctggcccatg cgtttcatgg
    21421 ggatcatcga cattgcctct ttcagggcgc tctcttccat ttcgatcatc ccggtatcaa
    21481 taagccccgg cgcgatgcag ttgacggtga ttttgcgctt cgcgagttcg atagccagcg
    21541 cttttgttgc gccgataatt ccggctttgg cagcgctgta attcacctgg ccgcgattgc
    21601 ccattacgcc ggaaaccgag gatagcgtga tgatgcgccc accctggcgt gcgccgatca
    21661 tcggcataat gcacggctga atgacgttgt agaaactgtc gaggttggtg tggatcaccg
    21721 catcccagtc gtcatcgctt aaagccggaa aagcggcatc gcgggcgatc ccggcgttac
    21781 tgaccacgcc gtaccaggca ccgtgttgcg cgatttcgtg ctccagcact tcccgacact
    21841 gttcgcggtt tgcgacgtcg aaactgagca aacgcccgtt acccccattg gcgacaatgg
    21901 cattcagcgt ttcctgagcg cctgtggcgt cacggtgata gtgaacgcca atgttaaagc
    21961 cgtctgccgc cagttggcag gcgatggcgc gaccaatgcc tttgctggca ccagtaacca
    22021 gaactgaacg actcatcagg acgctccttg ttgaaacagg gtggtaagtt cttccgcggt
    22081 cggctggaag gtgttaatgc ggcccgtcgc ctcgccgtcg ttgatgctgc actcaaaact
    22141 gccgaagcga tcgtcttgca tcagcagttt gacggtaatg gtcagtgtct ggcctgcggg
    22201 cagaatgcca gtggtgcaaa gcaattcacg cgcgcccagt atcatcccta actcaatact
    22261 gtttttgccc tgctggtggc gatgccagcc agaccagacg ccgacggttt gcgccatcag
    22321 ttcaagggta aaccagccgg ggagattgcc gtccgggtcg agaaacggtg ccagtacgcc
    22381 actgggcgaa accgtcacgc ggcacaccgc gctgtcatcg ctgacgctca ccacgtcttc
    22441 cagcagtaac atgggcgcgt cgtggggcag ataagcgccg ggggataaat agtggctcac
    22501 gaaaccctcc cgagcagaat gctggcgtta ttgccgccaa aggcgaagga attggacagg
    22561 atcaccggac gtgccagcgg ctgcggtttt tcgataatgc cacacggtgg cagcgtggga
    22621 tcgcgcggcg acaggctgaa gtcctgagcg ggcagcggta aatcacgctg taaaatcaac
    22681 atactaattg ccgcctcggt aatgcccgct gtgcccagcg tgtgtccggt caaatgtttg
    22741 gtggagctgc acggtacgcg ttcgccaaac agggcgttaa ccaccattga ttcaatctga
    22801 tcgttgagtt gtgtggcggt gccgtgcagg ttgatatagc cgacgtcatc tggcgtaagc
    22861 tgcgcatcgg tcagcgcctg attaatagcg cggatcgctc cttcgccctg cggatgcggg
    22921 gctgaaatat ggtaagcgtc gctcgattca ccaacgccca gcaacgcgat gggctgcggt
    22981 tcccgggtca gcagcattag ccccgcccct tcaccaatgg tgatacctgc gcggtcacga
    23041 ccaaacggct gacataaggt tggcgacagc gattcgaggc tgtgaaaacc gttaatcggc
    23101 atccggctca gggtgtctgc gccacccaca atggcggcat cgaccagccc cgcttcgata
    23161 agcctgcgcc cgctgattat cgctctggcg ctggaagagc aggcggttga aagggtatag
    23221 gccgggccgt cgagcgccag ccagtggctg agaaaacgcg acggatcgcc gagttcctgt
    23281 tgcgcgtagt gccatgtggt gctcggctgc ccgtgggtgc gcaggttaac gtgcgtgtcg
    23341 ccttcatgca ggccggaggt gctggtacca agcacaatcg caatgcgctc gcggccatat
    23401 ttcgcaatgg cgtcatcaac ctgcggctgg atctgcgcca gcgccgccag caggatctga
    23461 ttgttgcgcg agcggtgcgc agcgaatttt tccgggataa gcggaagctc gccatccacg
    23521 ccagccagga ccgcctgcgg atgcccctgc aaccagcctg cgcgcgggcg catgccggga
    23581 gcaacaccgc gagtcaggtt ggttgcgatt tcgtcaaggt tatttcccag ggcgttgatc
    23641 atgccgacgg cggaaatata aatcatatca gtcacctaag tattgaatgg tgatgtggta
    23701 tttaaagaca tgctgctcaa tgctgattgg cacgcgtttg cccttgcgat tcagataggt
    23761 gatttccgtg accagtttgc cgctggcgtt acgcagctcg cgtttgtcac cgttgtcgcg
    23821 aagtgtccat cctgcgggaa gttgcggttg ccaggcgcta atcggccagt ggctgagcat
    23881 cacgtcagcc agtacctgac ttgcgggcgg taactgtggg acgacgatgg attgctcggc
    23941 gcgtagccct tgcgcatcgt aggtcaccag aaacaggcga atgccgaccg acgacagccc
    24001 ggcaagggtg attttctgat cgtcggcgtt aagcatcact aacagcgact gggttttgcc
    24061 gttgaagctg ccggtcaaca gttgctgcga attgactgcg ggtgaaatcc ctggtgccgg
    24121 cagcgtgatg cgcgtgccag gttgcagcca cgcctgcggg cggccttcct gttctggttg
    24181 cgagtggcta cagccggtga ggataagcgt agcggtcagg gcgaacgctc gccagaatgt
    24241 ggatttgatc atttttttgt tctcttttta tcgggcatag ccagcggcga aagcaggaag
    24301 gcggtgaaaa taccgctcac cagcacaatg ccaaaactgc tgatggcctg ggtggcgctg
    24361 aataccagca tacccagcgt cagcaaggtg gtgagcattg ccagcgcgat cgccagtagc
    24421 gaagttaacg gtgtaccgcg cggattactg aaaaacagcg tgtagttgat accgatgcca
    24481 agcaccagca ccagcgccag cagcgaaaag agattcaccg cctgcccgct catcgccagc
    24541 accgccagac cacagcccag cgaaagcacc gaaggcacca ggctgataag ccctttgcgc
    24601 cagccgagac gggccactgc gccgcaggca atcactgcca gcgccaccag caacaagccg
    24661 gttaagacgt agcggtaaag tgcgaacaat tcatcaaagg tgcttttgcg atcaacccag
    24721 gcaatgccgc aagggtaata tgtggcgatt tcctgcatca acgcgctact tttaacccct
    24781 tcaaccggca ccagtacgcc gctttcgccg ttttccagcg tcagccacag cagacgccag
    24841 ccttcactgg cggggcttgc cagccattca tcaacgttca ctggcatggc gttgagatcc
    24901 gggttcactg ccgtcagccc ggcattttgc agcgcttttg ttactgtcgg ggccgccgtt
    24961 ttcagcagtt gtaaatcttc ctcctgccgc gcaagggagt tcagcggaat ggtgcggtag
    25021 ttgctgataa gcccctcttt tttcgcatac tcaagtgagg cggtatattt ctccagtcgc
    25081 cgcaatgttt gctgtggcga atcgccgtaa accacaaacc atttttgatc gacgctctgc
    25141 ccggtcaggg cggtaatggc tttttcctgc gccagaatat gctgcggtag cgcctgtaac
    25201 tgcgagatat cgtcatcgac gcgtagcatt gacatccccg ccagcgaaaa cagcgccagc
    25261 gcgacgggca gacccagcga cagtttttta ttacgccgcc acgctgccag ccagcgtagc
    25321 atcagcgcca tcgccggaac cggacgcacc ggcaggccac ggcacagcca cggatgccag
    25381 aacaggacgg tcagacagga ggcgctcaac ccgacggcgg caaaaatcgc catctggcga
    25441 atgccgggga agggggcgag catcataatc agatacgccg ccacggtggt gagcagcgcc
    25501 agtagcaagg cattgcgtac tttcgccagg ctttgccacg gcgaaacgtc gttgccgtga
    25561 accatccgct cggtgagata atagagcgtg tagtcagcgg aaatgccgat aacgctcatg
    25621 ctcatcacca gcgtcatcag gtgtaattca ccgaaaatca ataaagtggc gaccgttccc
    25681 gccagcgcgc cgatgccgat ggaaatcacg caaagcagca acgggcgtaa agagcggaac
    25741 accgccacaa tcagcaatat cacccccagc agcgtagcca cgcccagggt ggagatatcc
    25801 tgcttcgcct gttggctggc gtaatcgctg taaaacaccg tgccgcgcga gagcaactgt
    25861 gcctgcgggt aacgcgtttt cagatccttt tccagcgtat tcagggtcgt gatcagctgg
    25921 tgggtttgct gcatatcaaa cgacgatccc gccagttcgc cgtgcagcag ataccagtag
    25981 ttgccctggg gatcctgcgt caccagccaa ccgtccatca gccgcaaacg ctggccgttt
    26041 ttcgccattg ccagctgcga gccgcgcatt aacatcagcg gatcgttttg cagctccttg
    26101 ccgcttacgc cggagaatgc ggaataaagc tgggagagga tccactgtgc ctgcgcttcg
    26161 ccgccgtttt gcaggcgggc gcgggtgttg gggtcaatca ggccgttgcg atgctgccag
    26221 aaaaacgctc cccacgcctg ctggctggcg gcatccattg gccctttaac gtcgccgagc
    26281 gcagcggatt tttgcagcag cgtcagccac tcctgagcga ccaaaggatt agcctcttta
    26341 ccggggctga ccagccacac cagttggcgg tcaagacgct gcataaagcc gtcattcagc
    26401 gccggaggaa tatcgcccat cgtctgtttg ggtaacatag ccagcacgct actgttcagc
    26461 cgtgattgcg gcagcaggat cagcaacgcc accgccatga ccaggcagac tagcccccat
    26521 aacagcgcgg ggcgtttact gggcggcaaa acgttggcgt tcgtcatcgg tcagttgcgc
    26581 tggcgtcagt tgatgttggg taagagcaat atcggtgcga tcgccctgtt tatcattaag
    26641 ttgaatgctc tccagatagg ttttcccggc gagatcgatg gtgttgaaaa ttttatccag
    26701 cggcgtggtg gtcggcgtca ggcgcagcgt ccagcggcct tcgcctttgt cagcaaattc
    26761 gacgcggaag ttttgttcca gcactttgcg atcggcctgg aacaacgcgc gcagcaggtg
    26821 gttaaactgg aacatctgcg ggttgttttc tgccgtgatg atttgcggcg gctgaccgtt
    26881 gatcacctgc accatgcgtt tatcgtccag taatagttgc atggggaacg gtgaggtttg
    26941 atcccacaac agcccctggt cgcgggcgat caacatctgg ccctgagatc gcagcggctg
    27001 cggcagatct ttaatcgtcc gggtttgatc gaaatgggcg cggatcaccg gttgttcggt
    27061 aaagcgttgc tgaagatcgt ccagggtcag ggcgctcaca aacgggctaa tcagcagcgc
    27121 cagcagcggt aaaaatttca tggtttgact cccatgcgtt caaacagaat atcggggctg
    27181 acaaagcaca gttcgcgact ttgctcgtcg accgcgacct gaatggtgta tccggtggtg
    27241 gcgcgtttgc ccgtttcggc atcgaaaatc tgatagccaa tccgcagacg gttttcgaac
    27301 tcttcgatat gcgcacgcac gcggatgcgt tgctcaaagg tgagggcgtg gcgatatttc
    27361 acccgcgcat cgaccactgg ccacaaataa ccggactctt tcatttgccg atagccgtaa
    27421 ttgaactggt tgagcagcgc ctcgcgagcc acttcaaagt agcgaaaata gttaccgtgc
    27481 caggccacgc ccatcatatc gacgtcgtga aagggaatgg tcagctcgac ttctgcggta
    27541 aatcggggat cgttcagcac cctttactcc ttgtcctgaa tttccggcag ttgccagaaa
    27601 tcgaaaaaat taaaccagtc gagaggcgac tggagcgcgt aatgttccag acgcgcggcg
    27661 taatgatcga tagcgttttg cagcgcctgt tggcgttcgc cgcgcggcag ctgcagtggg
    27721 tcggcaaagc tttcgcagtg aatatgcagc ttgccgtgct ggcgcagggc gaaaatcaga
    27781 ttcaccgggc agcgcaaaat agaggcgaga ataaacggcc cctgtgggaa aggcgcaggc
    27841 tggcccataa acgaactcca gcagacgcgc cagtcgccgc cgcgttgcgg gttgacggcg
    27901 atgcggtcac cgacaatcgc cacccattcg ccgttatcca gcttctcttt cagcaggatg
    27961 gcggtttctg ggccgatatc tgttaccggc atcaggttaa tgcctgcctg aggagccatc
    28021 tcctgcatta tctgtttaaa gcgttgggcg ttttcgctaa acaccagcgc gttaatggtt
    28081 ttgtagccct gaatttttgc cagcgcccgg cacacttcca catcgccaag atgcgaggcc
    28141 agcagcaatt tgccgcgcgg atcgcggacg tcaagcgctg cttctgcgcc tggcgcaaac
    28201 agcacatcac gccctggttg tagctcgccg cgccagctgg cgattttgtc gagcatggca
    28261 ttaccgaaac gtagaaagtg ctgatagctg ttgagttttg ccgcctgtgg ttgatgctgt
    28321 cgtacacggg cgagccagtc ttgcgacgct ttgcgcgcac gagaagcagt gagccagtag
    28381 acgcccacca ccgggtaaag cagcgcgcta aacgccgttc ttcccagcag acgccagacc
    28441 agcagcatca ggcgcattcc ccataatcct ttcacttcac tctgccgcgc ccagtggcag
    28501 gaagagcggc gcatcagcag tgaaggaata cgcggcaaca tgccgaaaaa cagacgcgtg
    28561 tgcatgagcg agatgcggac gttatctttc agggcatcaa aatgcgaaag cccgtccagt
    28621 ggataggtga cgcgggtcgg cacgaaatag ctggtatttc cctgccagta gaggcgcacc
    28681 atcacttcgg tgtcgaaatc catccgcttg ccgatggtgg cgtgttttgc cagttgcagc
    28741 gttggcgcaa ccggataaac gcgaaaaccg cacatgctgt ctttcagttg cagggagagc
    28801 gtttcgatcc atacccagac gtgggtgacc cagcgcccgt aaagccgtga gcgggggatg
    28861 gagtcatcgt aaattggctg gccggagatc agtgccgcag gttgttgttc agcgagagcc
    28921 agcagtttag ggatatcttc aatcgcgtgc tgaccgtcag catccacctg caccgcatgg
    28981 ctgaaccctg cgtctgccgc tgcctgtaag ccacgcatca ccgccgcgcc tttgcctgcg
    29041 ttttctgcca ggcgaattaa ggtcacgcca ggctgttcgg caagcagact gtccagttgc
    29101 tgttgtgtgg tggcatcgct gccgtcatcc accacaatac agggcagatt aaatggctta
    29161 agacgcgcca gcacgcccgg catcatcgcg ccgtggttgt agcagggtat caacacgcag
    29221 ggagaaaagt ttaccgacat aggcggatct tcccgctact ggcggtgtgg cgggcgtcac
    29281 cgtcgtgacg ctgatagcta aagctcagaa tctggcgttc ttcctgccag ttaagcgcga
    29341 gcgtgacgcg gttttccggc agcaacggag actggaattt tatgttctga atggagtgaa
    29401 aacgccagcc aggcgcgagc agattgagcg cgtagctcat cgcccagtcg atttgcgcaa
    29461 ctcctgggag taatggttgc acggcaaaat gaccgcgaaa ccagaacaga tccgccctga
    29521 gatgcagaac aatttcaagg tgattcgttt gtgcctgatg gcgctcgatt tcatggggct
    29581 tcatgaaata actcctgtaa ttgcgcatag acacgcttgt tcatactgtt tactggcatt
    29641 tcatcaataa cgcgccagta acgcggaata gcaactggct ccagcgtcgg gcgcagtaaa
    29701 cgccgccagg tcatttcctg gctatgcccg ccgccgttct gccattgcag atgagcttcg
    29761 tcatccagca ccagcaaaac gcctatgctc tgacgcccgc cgcgcgtaac gggaactgcc
    29821 gccgcctcgc gaatgccgtc cagcgccagt agacgttgtt ctacttcctg gagtgaaata
    29881 cgtttttctt caatcttaac gatgcgccca cgacgcccca tcagatggaa ctgaccatct
    29941 tcgctaaact gtaagatatc gtcgagtaac atgccgttat cttccgctat cagcggtgaa
    30001 aagaggcgaa aggcatcgcc ttcggcctgg aactgcatac ctgggagggg tagccagcgg
    30061 cgttgctctt gttcgcggta gcgccaggcg atgacgccag tttccgtgct gccgtagatt
    30121 tcatcaggcc agacgcgcag ccagctcgcg gtatgttgta catcctgcca cggcagctca
    30181 ccgccagcag agagaagcac ctgaacgggt ggcggagaaa gctgggtatc caggcgcttc
    30241 aaaaatgccg ggctgctgat gaagatatag cgatgctgat gactcaacgc ggcaaactgt
    30301 tcgacatacc agagcatggc ggcgtgcagc ggtaatccca acgccatggg caggaaaacg
    30361 cgaaaagtca ggccgtacaa atgctgcggc aatacggaac cgacgacacg acaacccgcc
    30421 agacgtgcgc ctaatcgttc agccagcagt tctgcttcac gatccagcaa atgaacaggt
    30481 ttaatcgccc gtttcggctg tcccgtggaa ccggaggtaa acagctcgat aaaagcctct
    30541 ggcgctatag cggcaaaggt gaaggattgt gtcgcgattt gtggagaact tgctaccaac
    30601 agcaatgaac cttgccagtt aagttcgctg tcgctaagca caccatcaaa aagttcacgt
    30661 tgctcgttaa gctggataac acggttatgc cccggaagca ccggcgtttt tccggcgtgt
    30721 agcgtggcga gcagggcaac gataaacaga tagctgttct caaagcacag cgcccagcgt
    30781 tcacccggct gttgctgcaa gcggcagata agttgcgcga catcgtgacg caaatcgccc
    30841 agcgtccagg tactttcgtt cagccacgca atgggtgtgt catcagggcg tggcgcagtc
    30901 agccagcgtg caagcggtaa ggtctgcttc atgcgcgatc tcttttcatc acctgacggc
    30961 gcagcagcca ttcaccagcc attaacgtcc ccatcaacaa ataagcaatc atgccgttcc
    31021 aggcggtcca taacgacata tcggcataaa gcgcggtaaa gagggcaatt ccaccgttaa
    31081 tgatgaaaaa gccgcaccag atttgcgtga cgtggcgggt atagcgcacg cccttttccg
    31141 ggaggtctgg ctcctgcaac cgcgccagcc gctcaactat cggcattgcc gaccacagcg
    31201 aaccgccaaa aaccgccagc atgaccgcgt taaccactac cgggtaaaac agcagtagct
    31261 ggtgcgtttt caacataaag ctggcgacac ataatgcgat gcccacgaca gcgacgagtt
    31321 gcgtgacggc ttgtaaaggc cccgcctggc gacgggtctg gcgcagacgc aacaaaagca
    31381 gcaacgccat tagcggtaac agccagtgca ggccgttatg cgctaagcca aaccaaatca
    31441 caaacggcca ggcgagcagc aataatccag tcagcagctt aatcatcggt agcgagcgaa
    31501 tgcccgacat cagatcaggc ttcttgtagc aggcgttcta cagcctcgac gacatcctgg
    31561 actgtacgca ctgctttaaa ctcttccggc ttgattttct taccggtttt cttttgcagg
    31621 tgcacaatca tgtcaacggc gtcgatgctg tccagttcca gatcttcgta caggcgcgtt
    31681 tcaggtttga tgtcctgcgg atcgatttca aacagcttaa ccagcagggc tgagacttcc
    31741 tgatagacgg tttgttgttc tgtcatgatg gctttcctca ggcacgttgc gcagcaatga
    31801 aagaggccag agtggcgacg gagaaaaagt gctgacgcat ctcttcgctt tccgcagaga
    31861 gcacgatgcc gtactcattt tttactgcca gtcccagttc cagggcatcg atagagtcca
    31921 gccctaaacc gtcgccaaac agcggcgcgt cggtatcgat atcatcaggc gtcaactcgt
    31981 ccagattgag ggtgctgatg atgaggtttt taatttccag ataaagcgct tgcattattt
    32041 attcctgaga gaggtaaaca accttgttgt aattgaagca aaagatgccg gtttaactgc
    32101 cttgctgcca gcgccggttc ttgtgaagtt gcatcgtaaa atttattgat ttcaacccga
    32161 ccatggactg caaccgtaaa aaacggccgg tcaggtggaa cgtcatacca tttactttgt
    32221 ttatccagca gatgctcgct acagccgatt gtcacgatgc gtaaatcgct ggcgcagcgc
    32281 acggcaatat tcgccgcccc gcgctgcagc gtcatttttt caccgggctt cgttcgtgtg
    32341 ccttcgggga aaatcaggat agtgtcaccc tgtgccagtc gctgttggca gcggggtaac
    32401 agagtttcag cttcgctgtt gattaaataa tctgtcgccc gcacgacgcc gccaagaaaa
    32461 ggatttttta acagcgcgct tttcaccagg cagtcagttt ctggcatgac tgacgccagc
    32521 agcacgtaat cgatcagcgt gggatgattg gctacgacga ggcaaccgcg ttcctgatgt
    32581 aagatttcag cgccatcaat acgataatcc agtacgccaa gccctttcgc gacagttaaa
    32641 aacaggcgaa aactggcggc aatgctgcga cgcgccaaac gacggcggcg ggaagtatcc
    32701 cacaccaaaa ctaacaatat gttaaaccac acgacagaaa gcagcaggcc gccgagacca
    32761 aacagggcaa aacataaccc ggtcatgacc agtcgccagg tccactccag acgtgacatc
    32821 agcttactca tctgtggctc cagcgccatt gcacgcgctc gccaggcagt gaaaatgtgt
    32881 ctacattttg taaatagtgc tgtaaaaaca acatgctttg cggcagtgtt gtttcattgc
    32941 ccgcaatggc tgactgcgtt tcacattgcc agtcatcgcc agcttcaatc accagcgcga
    33001 cggcgtacgg ccaggtcggc atttctgatg gcagttgtgg gtgataaaac tcgggcagga
    33061 aaccgtcaaa atcgaccatc agcacgcgct ggtatcccgc ttgtagcagg ctaagcactt
    33121 cacacaaacc ttgctggaaa ctatcgcgac cagccgaaag cgatgacgaa acgctcggct
    33181 gtttggccgc aatggtcagg ttacccacgg aagagttgtg tacggagagc gcgaagtccg
    33241 tcggagaaag cgcctgttcg gttgccagtg catgaacaat gcggtaattg cgctccagtt
    33301 cgccatgacg actggtatac aaaacggcgt ctggttgatg gcggcgtagc attgccagac
    33361 cgcattcaac agccagtttg ctcccggagc tgaggcggcg ggcggtcatc attggcagtt
    33421 cacttaattt tgccaacggt gctgtcgggt caattgcctg aggctgacgt gaccactcct
    33481 gccattgcag cgtttcacta agtccaggcg ctcttgcctg ccagtcgaga atgtttaatg
    33541 caaatttcat caacgcgcgc ccgcggtaaa ccaaaattat taagtgacga ttatttcttt
    33601 tggcatatca aaagtgtatg cccaacgcct aaattatcgt ggcgctctgc tacctggaat
    33661 cctgctttat tcagatagtc gtaaaatttc tctgcactat agaaacggct gttaccgttt
    33721 gccatgcagg taaaataaag tgaggacgcg ttcaggctaa acgatgctgc ttcaaaccgc
    33781 tgcgcatccc agaacaactc catgatgcaa acttttgcgc caggcttcat tacgctggca
    33841 attttgctga gtatgctaat tatttgttcg ggtgaaaaac agtctaaaaa ttggctcatc
    33901 caccagatat cagcctcgcc cggtaatggg gcatcgctta gcatatccac cgcatggaaa
    33961 tctatgcgat cagaaaaacc tgcattcgcg atattttctt tcgctagtac gatttgttgt
    34021 ggcagatcta ataacgtaac ggcgatgttt tcattgtatt tgcagcaacg caaagcccac
    34081 ttacccgtat tgccgcctac atcgtacaat gttgtggggt tgttggcgaa tacataaggc
    34141 agtgcggcgt taaatgtgcc atctgaatag taatgatcaa acgcaaacca gctttcacga
    34201 gcggcgtctg gtaattgcga aagtgccgga tatatcgtcg accagtcgcc aaatactttt
    34261 aacccggaag gttttccttc attgagtgaa tcggcgagga agaagagccc ctggtaacag
    34321 acatcctggg tgaaatccat attcacgcga gtcatagtgt catgcaggag gtaatgaccg
    34381 attttcgcca ggtaataaat tccctcttta caggtaataa ttcgcccgct taagcccata
    34441 tctaacagaa cgctggtagc atattcgttg atatggctat gttcggtgat atcagccagc
    34501 gttgcgccat gtttgccttg ccgatccaga tatgaaagta cacccgcatt acgtaggcag
    34561 agtgctgttt gaaaaagcat aggggcaaag gcaatgcgtt gagcttcggt aatcgcatcc
    34621 agtgcgctaa gagaatcctg ttcgtacatg ttgggcacaa cctgttaaga gggaattaaa
    34681 ttattatcgg accgcaacgg cccgacttta ggaagaatta taaatctgcg cccgctgaca
    34741 aattcagttg aatatctttg tcgagattgc ggacccagcg attgtagttt ttatgaattt
    34801 gtccgcctga cgcttgtagg ttctggctat tttcataatt gatatcgtag ctggatgccg
    34861 tgtagttaat gcgtatttcc gctgaatgat cgcgtgactg ttgacgtgct ttaattatgc
    34921 cttgtccggc ttcggacata atccatttgc gttcaacgcc agctttcaga atcgcttttt
    34981 tcacttgttc ctgggtatga ccagcgctga cggttgagtg aacctgatca attggcgcag
    35041 tacgcgcgca acctgctaaa gcacccacaa cagcaatagc cgcaaaccat ttcatgtaat
    35101 gtctcattag aaccccatta aagtattgtt tttacgagta aagcaagttt taactgtaat
    35161 tgctggcatc actattcttt atttagagaa aaaattactt gtgagataag tcagtaagtg
    35221 cacatcagag taaaaatctc tgaaatgact aaattactgc cagcagggac caactataag
    35281 tgagtgagaa aaatgaggca ataaaaataa cacaatgtta tcagtgcatt ctttgagaag
    35341 gatttaatcg ctattcggcg tttatatata tttgacttaa ggaatggttt ttttaaatta
    35401 attttctgaa taatgtttca tcatttcctc aaatcaaatg gccactgtta gataaacggt
    35461 ggccattgcc tgctcaggag gtgctattaa cccggtaacc gacttttcgg tctgcccggc
    35521 cccagtaaaa tcgccagttt gctgccgcct ttcgtcgttt ccatccagat tttacacacg
    35581 ctggttaatg gcaccgaaag tagcatccct accgggccga gcagccatcc ccaaattaac
    35641 aatgaaagaa ataccaccat ggtggacatc cccaggcgat ggcccatcat ccgtggttct
    35701 aaaatattgc cgatgaccat atggaccact aaaaacaatg cgccgaccag aatacattcg
    35761 taaacaccat taaacagcag cacctgaatc attggcggta cggcggaaat taccgcgccg
    35821 atattgggca cgtagttaag caaaaacgcc agtactgccc acatcagcgc aaactgtacc
    35881 cccatcagct ccagccccag ccagacgatg acacccgtcc ataaactgag tagcgtcttt
    35941 aacgccagat aatgcgaaac gcctttaagt gcgcggtgta atcccgcgat gtgaatctgt
    36001 gggttattca gcgcaaaacg cattttgtaa gggacgtggc gcacttcaaa cagcataaaa
    36061 actacggtca tcaccagcaa aagcacgctc gccattgccc cggaaagccc ggtcattagc
    36121 gctgtggtga aggtaaccac tttttccgag tccatccgct gcagcattcg ctccggcgac
    36181 atatgcaaat taagaaaagg caacatctcc tgcaatttaa aaagtttgcg cgtcagctcc
    36241 ttattaaact tcggcagcat agagataaat tcgttaaacg atgccgccag tacgccgacc
    36301 agcgcggtta gtgcgatcag catcaccacc actacaatcg taatggcaac ggggcgttgt
    36361 actccccgac gaataaacca ggtgacgagc gggttgagga cgatggcaaa aaacagcgcc
    36421 agtaacagct gcacaatgat atctgccgct gcgtgaatgc ccgcgaggat cactaccagc
    36481 gaggccagct tgagcagaat gtgcatgccc gttttatcgg gttgaggggt ttccattggg
    36541 gcttccttgt gactttttgt attaagtgta gcgggagtca cgacggcaat attctgattc
    36601 ttactgctga ttttcacgtc agcacgtggt aaaaatgaaa cactgttgta aaaatgtggt
    36661 gatcctcatg cccgaacccg tagccgaacc cgcgctaaac ggattgcgcc tgaatttgcg
    36721 cattctctcc attgtcatgt ttaacttcgc cagctacctc accatcgggt tgccgctcgc
    36781 tgtattaccg ggctatgtcc atgatgtgat gggctttagc gctttctggg caggattggt
    36841 tatcagcctg caatatttcg ccaccttgct gagccgtcct catgccggac gttacgccga
    36901 tttgctgggc cccaaaaaga ttgtcgtctt cggtttatgc ggctgctttt tgagcggtct
    36961 gggatatctg acggcaggat taaccgccag tctgcccgtc atcagcctgt tattactttg
    37021 cctgggacgc gtcatccttg ggattgggca aagttttgcc ggaacgggat cgaccctgtg
    37081 gggtgttggc gtggttggct cgctgcatat cgggcgggtg atttcgtgga acggcattgt
    37141 cacttacggg gcgatggcga tgggtgcgcc gttaggcgtc gtgttttatc actggggcgg
    37201 cttgcaggcg ttagcgttaa ttattatggg cgtggcgctg gtggccattt tgttggcgat
    37261 cccgcgtccg acggtaaaag ccagtaaagg caaaccgctg ccgtttcgcg cggtgcttgg
    37321 gcgcgtctgg ctgtacggta tggcgctggc actggcttcc gccggatttg gcgtcatcgc
    37381 cacctttatc acgctgtttt atgacgttaa aggttgggac ggtgcggctt tcgcgctgac
    37441 gctgtttagc tgtgcgtttg tcggtacgcg tttgttattc cctaacggca ttaaccgtat
    37501 cggcggctta aacgtagcga tgatttgctt tagcgttgag ataatcggcc tgctactggt
    37561 tggcgtggcg actatgccgt ggatggcgaa aatcggcgtc ttactggcgg gggccgggtt
    37621 ttcgctggtg ttcccggcat tgggtgtagt ggcggtaaaa gcggttccgc agcaaaatca
    37681 gggggcggcg ctggcaacct acaccgtatt tatggattta tcgcttggcg tgaccggacc
    37741 actggctggg ctggtgatga gttgggcggg cgtaccggtg atttatctgg cggcggcggg
    37801 actggtcgca atcgcgttat tactgacgtg gcgattaaaa aaacggcctc cggaacacgt
    37861 ccctgaggcc gcctcatcat cttaaaactt actgaataac cagcgtatta atgatgtttt
    37921 ctgcggtggt ctgcgctttt tgctgatcgt cagctggcag agtaatttgc atggtcagca
    37981 gttgattacc cacgttaccc agaataacgg aagagtacgc cgtctggcct ttcgcggaga
    38041 taatactgtc taactgctgc attttgtgac ctttcagctc aatggcttta ttggtgacca
    38101 cttgcagctg tggatcgcgg ctacgttgct gatcttccag acgcttcgcc agcaccgcca
    38161 gatcttcttt cggatcatcg cccatgatga cgatgactgc tttctgcccg gtggcgtcgg
    38221 accagacatg catgttattg gcctgcgttc ccagcttacc gctctggtcg gtcatatccg
    38281 ctggcagcga gaaacttaac ttgccatcaa gcaggttgac gggattcccg gtagcgttac
    38341 tttccgcgac cgaaccctgc gccgtagcgt tagtgtcttt atcatcacag gccgcaagcc
    38401 ccataaccag caggccaatt ccgacatatt taaccagatt gcgcattgac ttcttccttt
    38461 cgataaacgg ccataacggc tcattcatcc atcttatcac aactctgata acgaaccttt
    38521 aactcgcctg caaagcgttg atttcggatt tatctgccag tcttttcaac agcatattga
    38581 gtaatacgcc atacattggc aggaagaaaa cgatactgat taacactttg aaacagtaat
    38641 cgaccagcgc gatttccatc cagtgttcag ccataaaggc atccgggcta cgccagaagg
    38701 caatgaagaa aaaggccagc gtgtcgctga cgttaccgaa cagtgtggac gctgtcggtg
    38761 ccagccacca gcggcgactc tgacgcaggc ggttaaaaac gtgcacgtcg aggatctgtc
    38821 ccagcgcgta ggccatgaaa ctggcggtgg cgatacgggc gacaaacagg ttgaagtggg
    38881 cgagtgcgcc gaatccctgc catgaaccca tatagaatag cgacgagatg acgtaggaga
    38941 tcagcaatgc cgggatcatc accgcaaaga tgatacgacg ggccagcggt gcgccaaaaa
    39001 tacgtacggt caggtcggta gcgagaaaga taaacggaaa gctaaacgcg ccccaggtgg
    39061 tatggaaacc aaaaatggag acgggaagct gaaccagata gttactggag gtgatcacca
    39121 gcagatgaaa taacgataac cagaacaacg ccttatagcg ttgagtttgc gagaaaacgt
    39181 tcatattgta cctttttgat taaccattgg ggtgagggaa cccaatacgt acgacacgtc
    39241 tgttacctta acataataac gtgcttaccc ttttttcgag ccgccgcatg atactgcttt
    39301 acgttgacaa tgcaatggtt aattttcacg caatcgttaa cctggtttgc ttacggactc
    39361 acagggcgta aactagcgcc gtttttttat gtgatgagaa gaaaatgacc gatctctttt
    39421 ccagccctga ccacacactc gacgcacttg gcctgcgctg cccggaacca gtcatgatgg
    39481 tgcgcaaaac agtgcgcaat atgcagccag gtgaaacatt gctgattatt gctgacgatc
    39541 cggcgacgac ccgcgatatc cctgggtttt gtacctttat ggaacacgaa ctggttgcca
    39601 aagagacgga tggactgcct tatcgttatt tgattcgtaa aggcggttga cgggggcaga
    39661 ttggcttcga tgccgccttt tcccctcacc ctaaccctct ccccagagga gcgaggggac
    39721 cgatcgcgct caatgttgcg atcggtttgc cttatctcct gcgcaacaat cttaacgcat
    39781 tcgctgtcac cagcaccgtc gcacccgtat ctgccagcac tgccagccat agtccggtca
    39841 tccctaacag cgtggtgacg aggaagatcc ctttcagccc cagcgcaata gtgatgttct
    39901 ggcggatatt ggcgtgagtg gcgcgtgcca gttcaatcat ttgcaccaga ccgcgcaggt
    39961 ggttatgggt taatgctgcg tcggcggttt ccagcgccac gtctgtgccg ctgcccattg
    40021 caatcccgat ggcggcagct ttcatcgctg gcgcgtcgtt aataccgtca ccgaccatcg
    40081 ccagcggcgc atgttgattc agctcggtca ccgctttgac tttatcttcc ggcaacaggc
    40141 ccgctttaaa ctccagcccc agctccccgg caattgccgc cgctgcgcgt ggattatcgc
    40201 cggtgaggat cacccctttg acgcccagcg cgttcagttc actgatggca gttgcagcat
    40261 cggcgcgcag ggtatcctgc aatgcaatga tacccagcac gtcatcatta cgtactacca
    40321 gcacaaccgt ttgcccggcg ctttccagtt cattaatcaa accagcaaat gcatcagcgg
    40381 gatgtttccc ggcagcgcat atcaacacgc gctcaccgtt aacctgcgct tcaatgccag
    40441 acccgaccag cgcccgctgt gattcggcgg cgggaatggc gagtgcagca acctgtgctt
    40501 cgcgcacgat ggcctgcgcc agtggatgcg tcgcgccttg ctcgaccgcc gccgccagtg
    40561 tcagcagttc agattcacta atacccgttg ccggatgaat cgcggtaacg cgcggtttac
    40621 cgacggtcag cgtaccggtt ttatcaaacg ccacctgggt aacacgaccc agctgttcca
    40681 gcgccgctcc gcctttaatc aacgccccac gacgcgctgc cgccgccagc ccggaggtaa
    40741 tcgccgcagg cgttgagata actaacgcac acgggcagcc aatcagcagc agcgtcagcc
    40801 ctttataaat ccactcctgc cagctggtgg caaacagcag cggtggcacc agcgtcacca
    40861 gcagagcgac ggccataatc gcgggcgtgt aaatacggct gaaacggtcg ataaaccgct
    40921 caatgggtgc gcgacgctct tcggcttctt cgatcagttt cagaatccgg tcaatggcgc
    40981 tggcacccgg ttctgacagc acttccagcg ttaccagacg gtctacgctg gtggctcctg
    41041 caggaacttt atcgcccgtt gcgcgctcca ccggaataga ttcgccggtc agggcgcttt
    41101 catcaaaact ggcaaacggt gagagcagtt taccgtcggc aggcaaacgt ccacctgcgg
    41161 cgacttcaat cacatcgcca gggcgcaggc tgttaatcgc cacctcttcc cgctcaccgt
    41221 tacgcaggcg cgtggcggtt tctggtttca gcgccattaa cgcgctgacc ccttgacgcg
    41281 cgcggctggc ggcccagcct tccagtcgtt caccaatcaa aaacagcagc aacaccatcg
    41341 cagcttcagc cgttgcgcca ataaacagtg caccaatagc ggctacgctc attaaggttt
    41401 caatggcgaa gtagctgccg gatttgatca gccgtaatgc ctgacgagca atcgggtaca
    41461 gcccaaccag cgtggtcgcg ataaacgcca gttgcccgaa cggatgatta aattgctcca
    41521 aaccccagct gattgccatc atcacgataa gcgtaatcag cggcagattc tctttcaggc
    41581 gcgatgcttg tggctcgtcg gcggcctgct catcgcgcag ggaatagccc gctttttgca
    41641 ccgcagattc aacttgtgca cggatgtcat tgtcggcatc aaccaccagt ttttcggtgg
    41701 cgaacaacac ctgtacctga ttcacgcctg caagctggcg cacggcattt tctaccttgc
    41761 gcgcacaggc ggcgcagtcc atgccgctga ctttccagct atagcgggtg ccggagacgt
    41821 tttcagagag agttggcgtg ctggaacatg cgccgtcgca gcaacagtcg ttggcgttct
    41881 gtaccgtggt tagcggtttg aacgcagcaa attgaggggc tttcttgccg tgattgtcag
    41941 gagtcgacat ggcatcctcc ggttaagttt tttctcatta accgaaggat acactctgga
    42001 gtcgactcca gagtcaagtt ttatcagaga tacagcgagc ggacgatgag gaagtgaccg
    42061 gcaaagtagc aggccgcagc aatcgcgttg tccgcacgga agcgtcggcg atagtgacta
    42121 cccagccaga caaagttact gataaacagc aacgaagcgc cgacaaacgc agagagtgcc
    42181 ggagcggtcg gacggaagaa ccacagttca cctgccagcc acaccatcac cagcgtcatg
    42241 ccgataaacg tgcagatagg ccaacgatac tcttccagcc gcgtccagat aatcgccagt
    42301 aacagcgcac ccagaaccag caacaccagt ggcagaggcc agaagaaaga gagcgtcatc
    42361 tgactggcga aatagatggt gtacagcagg tgcgagagga aaaacgcgcc gatggcgtac
    42421 atcagacgtt gacgtggcaa cagggttagc gcatcgccca gcagtgaggc gcacagccct
    42481 gccagcacca gatagctaat ggcgtcgaac atcggcgctt gccaggccag taacagcagg
    42541 agaagaaggg ttaacggttt aaacacccag cgttgccagg ttggcccacg atacgatgca
    42601 tccacagata gccatgcgga aagacagaca gcgataaacg accaaagcat cttagttcct
    42661 tgaattcgtt attttttcgt aagcggaatg cctacggtct tgcttccagt ttagtggcga
    42721 gggtggacgg atgacaacac cataactggc agggtatgct tatttccgca ttttccgatg
    42781 agggataaag atgagcaaac cacctctttt ctttattgtt atcattggct taatcatcgt
    42841 cgccgcatcg tttcgtttta tgcaacagcg acgggaaaaa gctgataatg atatggcacc
    42901 actccagcaa aagctggtgt tggtgagcaa caagcgggaa aaaccgatta acgatcgccg
    42961 ttcgcgccag caggaagtga ctccggcagg caccagtatg cgctatgagg caagcttcaa
    43021 accgcaaagc ggaggaatgg agcagacgtt tcgcctcgac gcccagcagt accacgccct
    43081 gacagtgggc gataaaggta cgctgagcta taaaggaacg cgctttgtta gctttgttgg
    43141 cgaacaataa ggttattttt taactttaaa tttcttctgc cagaccagca gttcaaacac
    43201 gccgaaaagg aaaatccgca ccttttcggc ggtggtcatc tgcgggccat ctttcggtaa
    43261 agtggatttc aatagcgcca gttgcatacc atgcatcagc acggtaaaaa tcagcgccac
    43321 gttaacgaaa atattcagcg ggcgcgggaa gggatgcacc aggttgagga ttaaaaatcc
    43381 ccaaacacag agcattaaca aacgaccaat attaatcagc atcactttct ccttgtgctt
    43441 cgcgttgata cagccgataa gccacctgac ccgccacttt ttcccgatgt aacgaccagt
    43501 ttgccggaac ggtgggcaga ccgttttcga cttcgctttc gacataaatc agggtttcgt
    43561 cagccagcca gccgttatct tccagtaaat ttatcgtctc ttctaacaaa ccacggcgga
    43621 acggtggatc gacaaacacg atattatgcg gtgtgccttt ttgcgccagg aatgacattg
    43681 cgttgctgtt caccacgcgt gcattgcctg cttttagtgt cgccagattc ttaattaact
    43741 gctgagaaac tgcacgatcc atctcaatta acgtggcccc cgcagcgtag cgcgataacg
    43801 cttccagccc cagcgcgccg ctcccggcga agcaatccag acattgggcg tcaacaatga
    43861 ccggagccag ccagttaaac aacgtttcgc gtacgcggtc ggtggtgggg cgcagacctg
    43921 ggctatcagg aaccgggagt ttacggcctc gccactgccc gccaataatg cggatttggc
    43981 cgctgccgga atgattcggt tttttcatga taaattgctc aatccgccag ataacacata
    44041 cttgcaggcg gtgatgtgaa ttaagttaag tgatagacta tttcatcatt tttttagctg
    44101 ctatgtacat agcgttaacg ctgtgccatg aagcaacagc gaggagtgta gtcgcaaatg
    44161 gcgaaagaaa aaaaacgtgg ctttttttcc tggctgggct ttggtcaaaa agagcagacc
    44221 ccggaaaaag agacagaagt tcagaatgaa caaacggttg tagaagaaat cgttcaggcg
    44281 caagagcctg tgaaggcttc tgaacaagcc gtcgaagagc agccgcaggc gcatactgag
    44341 gccgaggcgg aaacttttgc tgccgacgtt gtggaagtca ctgaacaggt tgctgaaaat
    44401 gaaaaagcgc agcctgaagc ggaagtcgtt gcacaaccgg aaccggtcgt agaagaaacg
    44461 ccggagccag tggctatcga acgtgaagag ctgccgttgc cggaagacgt caacgccgaa
    44521 gaggtttcgc cagaagagtg gcaggctgaa gcggaaaccg tagagattgt cgaagcggcg
    44581 gaagaagaag cggctaaaga agaaatcacc gacgaagagc tggaagcaca ggcgctggct
    44641 gccgaagcag cagaagaggc ggtgatggtg gttcctccgg cagaagaaga gcagccggtg
    44701 gaagaaatcg ctcaggagca ggaaaaaccg accaaagaag gttttttcgc gcgcctgaaa
    44761 cgcagcctgt taaaaaccaa agaaaatctc ggttccggat ttatcagcct gttccgcggt
    44821 aaaaaaatcg acgatgatct gtttgaggag ctggaagagc agcttttgat cgccgatgtg
    44881 ggcgtggaaa ccacacgtaa aattatcacc aatctgacgg aaggcgcatc ccgcaagcag
    44941 cttcgtgatg ccgaggcgct ctatggcctg ctgaaagaag agatgggtga gattctggcg
    45001 aaagtcgatg agccgctgaa tgttgaaggt aaaacgccgt tcgtgatcct gatggtgggc
    45061 gtcaacggtg tgggtaaaac cacgacgatt ggtaagctgg cacgtcagtt tgagcagcag
    45121 ggtaaatcgg tgatgctggc ggcgggcgat actttccgtg cagcagcggt tgaacagctt
    45181 caggtctggg ggcagcgcaa caatattccg gtgattgccc agcataccgg ggcggattcc
    45241 gcctctgtta tcttcgacgc cattcaggcg gctaaagcgc gtaatatcga cgtcctgatt
    45301 gccgataccg ccggacgcct gcagaacaaa tcgcacctga tggaagagtt gaagaaaatc
    45361 gtccgcgtga tgaagaaact cgacgttgaa gcgccgcatg aagttatgct gactatcgat
    45421 gccagcaccg ggcagaacgc ggtaagccag gccaaactgt tccatgaagc cgttggctta
    45481 accggcatca cgctaacgaa actggacggc acggcgaaag gcggggtaat tttctcggtg
    45541 gctgaccagt ttggtatccc tatccgctac attggtgtcg gcgaacgtat tgaggatttg
    45601 cgtccgttta aggcggacga ctttatagag gcactttttg cccgagagga ttaacaatga
    45661 ttcgctttga acatgtcagc aaggcttatc tcggtgggag acaggcgctg cagggcgtta
    45721 cgttccatat gcagccgggt gagatggcgt ttctgaccgg tcattccggc gcagggaaaa
    45781 gtaccctcct gaagctgatc tgtgggattg agcggcccag cgccgggaaa atctggttta
    45841 gcggccatga catcacgcgt ctgaaaaacc gtgaagttcc gtttctgcgc cgccagattg
    45901 gcatgatttt ccaggatcac catctactga tggaccgtac tgtctacgat aacgtggcga
    45961 tcccgctgat tatcgccggt gccagcggtg acgatattcg ccgccgggtg tcggcggcac
    46021 tggataaagt cgggctactg gacaaagcga agaacttccc tattcagctt tctggcggtg
    46081 aacaacagcg tgttggcatt gcccgcgcgg tggtgaacaa gcccgcggta ctgctggcgg
    46141 acgaaccgac tggtaacctg gacgacgcgt tgtcggaagg gattctacgt ctgtttgaag
    46201 agtttaaccg cgtaggggta accgtattga tggcaacgca cgacatcaac ctgatttcac
    46261 ggcgttccta tcgcatgctc accctgagcg atggtcactt gcatggaggc gtgggccatg
    46321 aataagcgcg atgcaatcaa tcatattcgg cagtttggcg ggcgtcttga tcgcttccgt
    46381 aaatcggtcg gcggctcagg cgacggcggt cgtaacgcac cgaaacgcgc gaaatcctcg
    46441 ccaaaaccgg taaatcgcaa aaccaacgtt ttcaacgaac aggtgcgcta tgccttccac
    46501 ggcgcattgc aggatctgaa aagcaaaccg ttcgccacat ttttaacggt gatggttatc
    46561 gccatctccc tgacgctgcc cagcgtctgt tatatggtgt acaaaaacgt taaccaggcg
    46621 gcgacgcagt attatccgtc accgcaaatc actgtttatc tgcaaaaaac gctggacgat
    46681 gacgccgctg cgggcgtggt ggcacagttg caggccgagc aaggcgtaga gaaagtgaac
    46741 tatctttctc gtgaagacgc actgggcgag ttccgtaact ggtctggttt tggtggcgcg
    46801 ctggatatgc tggaagaaaa cccgcttccg gcagtggcgg tggtgatccc gaagctcgac
    46861 tttcagggga cggagtctct gaatacgctg cgtgatcgta tcacgcagat taacggcatt
    46921 gacgaagtgc ggatggatga cagctggttt gcccgtctgg cggcgttgac cgggctggtc
    46981 gggcgcgttt cggcgatgat cggcgtgttg atggtggcgg cagtgttcct cgtcatcggt
    47041 aacagtgtac gcctgagtat ctttgctcgt cgtgactcca ttaacgtgca gaaactgatt
    47101 ggtgcaacgg atggtttcat cctgcgcccg ttcctgtacg gtggcgcatt gctggggttt
    47161 tctggcgcat tgttgtcatt aattttatca gaaattctgg tactgcgatt gtcatcggcg
    47221 gttgcggaag tggcacaggt tttcggaacg aagtttgata tcaatggttt atcgttcgat
    47281 gaatgcctgc tgttgctgct ggtatgctcg atgattggct gggtggcagc gtggcttgcc
    47341 acggtacaac atttacgcca ctttacgcct gaataataaa agcgtgttat actctttccc
    47401 tgcaatgggt tccgtagcag ggaaagagac cccgttgtct cttcccggta tttcatctct
    47461 atgtcacatt ttgtgcgtaa tttattcaca agcctgcatt gaacttgtgg ataaaatcac
    47521 ggtctgataa aacagtgaat gataacctcg ttgctcttaa gctctggcac agttgttgct
    47581 accactgaag cgccagaaga tatcgattga gaggatttga atgactgaca aaatgcaaag
    47641 tttagcttta gccccagttg gcaacctgga ttcctacatc cgggcagcta acgcgtggcc
    47701 gatgttgtcg gctgacgagg agcgggcgct ggctgaaaag ctgcattacc atggcgatct
    47761 ggaagcagct aaaacgctga tcctgtctca cctgcggttt gttgttcata ttgctcgtaa
    47821 ttatgcgggc tatggcctgc cacaggcgga tttgattcag gaaggtaaca tcggcctgat
    47881 gaaagcagtg cgccgtttta acccggaagt gggtgtgcgc ctggtctcct tcgccgtcca
    47941 ctggatcaaa gcagagatcc acgaatacgt tctgcgtaac tggcgtatcg tcaaagttgc
    48001 gaccaccaaa gcgcagcgca aactgttctt caacctgcgt aaaaccaagc agcgtctggg
    48061 ctggtttaac caggatgaag tcgaaatggt ggcccgtgaa ctgggcgtaa ccagcaaaga
    48121 cgtacgtgag atggaatcac gtatggcggc acaggacatg acctttgacc tgtcttccga
    48181 cgacgattcc gacagccaac cgatggcacc ggtgctctat ctgcaggata aatcatctaa
    48241 ctttgccgac ggcattgaag atgataactg ggaagagcag gcagcaaacc gtctgaccga
    48301 cgcgatgcag ggtctggacg aacgcagcca ggacatcatc cgtgcgcgct ggctggacga
    48361 agacaacaag tccacgttgc aggaactggc tgaccgttac ggtgtttccg ctgagcgtgt
    48421 gcgccagctg gaaaagaacg cgatgaaaaa actgcgcgct gctatagaag cgtaatttcc
    48481 gctattaagc agagaaccct ggatgcgagt ccggggtttt tgttttttgg gcctctacaa
    48541 taatcaattt cccctccggc aaaacgccaa tccccacgca gattgttaat aaactgtcaa
    48601 aatagctatt ccaatatcat aaaaatcggg tatgttttag cagagtatgc tgctaaagca
    48661 cgggtagtca tgcataaaac gaaataaagt gctgaacaac aacatcacaa cacacgtaat
    48721 aaccagaaga atggggattc tcaggatgaa cataaagggt aaagcgttac tggcaggatg
    48781 tatcgcgctg gcattcagca atatggctct ggcagaagat attaaagtcg cggtcgtggg
    48841 cgcaatgtcc ggtccggttg cgcagtacgg tgaccaggag tttaccggcg cagagcaggc
    48901 ggttgcggat atcaacgcta aaggcggcat taaaggcaac aaactgcaaa tcgtaaaata
    48961 tgacgatgcc tgtgacccga aacaggcggt tgcggtggcg aacaaagtcg ttaacgacgg
    49021 cattaaatac gtgattggtc acctctgttc ttcatcaacg cagcctgcgt ctgacatcta
    49081 cgaagacgaa ggcattttaa tgatcacccc agcggcaacc gcgccggagc tgaccgcccg
    49141 tggctatcag ctgatcctgc gcaccaccgg cctggactcc gaccaggggc cgacggcggc
    49201 gaaatatatt cttgagaaag tgaaaccgca gcgtattgct atcgttcacg acaaacagca
    49261 atacggcgaa ggtctggcac gagcggtgca ggacggcctg aagaaaggca atgctaacgt
    49321 ggtgttcttt gatggtatca ccgccgggga aaaagatttc tcaacgctgg tggcgcgtct
    49381 gaaaaaagag aatatcgact tcgtttacta cggcggttat cacccggaaa tggggcaaat
    49441 cctgcgtcag gcacgcgcgg cagggttgaa aactcagttt atggggccgg aaggcgtggc
    49501 taacgtctcg ctgtctaaca ttgcgggcga atcagcggaa gggctgctgg tgaccaagcc
    49561 gaagaactac gatcaggttc cggcgaacaa acccattgtt gatgcgatca aagcgaaaaa
    49621 acaggaccca agcggcgcat tcgtttggac cacctacgcc gcgctgcaat ctttgcaggc
    49681 gggcctgaat cagtctgacg atccggctga aatcgccaaa tacctgaaag cgaactccgt
    49741 ggataccgta atgggaccgc tgacctggga tgagaaaggc gatctgaaag gctttgagtt
    49801 cggtgtgttt gactggcacg ccaacggcac ggcaaccgat gcgaagtaac agatagataa
    49861 atgaaaatgc ccgatgcgct gaactgccat cgggtttttt tgtgtttgtc gaaatggttg
    49921 tagaaagatt aaacaatcca ggatatgctt aatgtacggt gacataccgt acatattata
    49981 cggagcagga gagttcacat gtctgctgtt aaaaagcagc gtatcgatct gcgtttaact
    50041 gacgatgaca aaagtatgat tgaggaagcg gcagcgatat ctaatcagtc cgtcagccaa
    50101 tttatgttga acagcgcttc gcagcgggct gcggaagtga ttgaacagca tcggcgggtg
    50161 atcctcaatg aggaatcctg gacgcgggtg atggatgcgc tgagtaatcc accgtcacca
    50221 ggtgaaaagc taaaacgtgc ggcaaaacgt cttcagggaa tgtaataatt tatggatgat
    50281 ctgacgatag agattctgac cgatgatgca gattatgatc tacagcgatt cgactgcggc
    50341 gaggaagcgt taaatctctt tctgacgaca catctcgttc gtcaacatcg caacaaaatt
    50401 ctgcgagcgt atatcctttg tcgcaacact ccagaacgtc aggtgctggg atattacaca
    50461 ttatgcggca gttgttttga acgagccgca ttgccctcga aatcgaaaca gaaaaaaatt
    50521 ccctacaaaa atattcccag cgttactctt gggcgtctgg caattgatcg ttcattacag
    50581 gggcagggat ggggagcaac actggttgct catgccatga aagtcgtctg gtcagcctct
    50641 ttagcggtag gtattcacgg tctttttgtc gaggcgctga atgaaaaagc ccatacgttt
    50701 tataaatcgc tgggctttat ccctttagtc ggggaaaacg aaaatgcgtt atttttccca
    50761 accaaatcca ttgaactgct ttttacacag agcgattaac gcttctccca gccgccctgc
    50821 tgtgccgtaa atcccagtgc ctgcataaac gccgtcatca caccgcgatc ttccacgcct
    50881 gcatccgcca tccaccagca tgaaacgcca ggattgttac gcaaaacctc ttccagcaga
    50941 tattgcccca caccgcgacg tcgagtgact tcccgcacgc gcagggaatc cagtgccccc
    51001 tcggtgccgc ttaaggttac ccgcacggca gcaagcaggc gctcgttaaa acgcgcggcg
    51061 tagatgcggt ggttatcgtc aacctgtaaa gaggaagggg aatactccgg ccagatcttt
    51121 tgcaggtcaa tccggtcctg gtcgctaaat ttttctaatc gaatgatggt cagcttcatg
    51181 ggtaacccgt gtaaatcaca aaagtggaac cagtgtagcg aaataattta atcggaggct
    51241 ttctcttttt tatttctttt ggcaggtgat taatttctta acagcaataa ttacaaagtt
    51301 aaaacattag agaatgaaaa atacccagca taatcccctg aatggtagtg aattattccg
    51361 ccctttgtgc cgttatttta tgctgacaaa
//
LOCUS       DAZFDC010000016        50008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFDC010000016 DAZFDC010000000
VERSION     DAZFDC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.016"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            150..2291
                     /locus_tag="ACHJ48_001105"
     CDS             150..2291
                     /locus_tag="ACHJ48_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO4706710.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2355..4247)
                     /gene="parE"
                     /locus_tag="ACHJ48_001106"
     CDS             complement(2355..4247)
                     /gene="parE"
                     /locus_tag="ACHJ48_001106"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO4706711.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGNCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4276..4857)
                     /gene="yqiA"
                     /locus_tag="ACHJ48_001107"
     CDS             complement(4276..4857)
                     /gene="yqiA"
                     /locus_tag="ACHJ48_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO4706712.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4857..5684)
                     /gene="cpdA"
                     /locus_tag="ACHJ48_001108"
     CDS             complement(4857..5684)
                     /gene="cpdA"
                     /locus_tag="ACHJ48_001108"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO4706713.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPNVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5709..6131)
                     /gene="yqiB"
                     /locus_tag="ACHJ48_001109"
     CDS             complement(5709..6131)
                     /gene="yqiB"
                     /locus_tag="ACHJ48_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO4706714.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6132..6761)
                     /gene="nudF"
                     /locus_tag="ACHJ48_001110"
     CDS             complement(6132..6761)
                     /gene="nudF"
                     /locus_tag="ACHJ48_001110"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO4706715.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6966..8447
                     /gene="tolC"
                     /locus_tag="ACHJ48_001111"
     CDS             6966..8447
                     /gene="tolC"
                     /locus_tag="ACHJ48_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO4706716.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASS
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8595..9266
                     /gene="ygiB"
                     /locus_tag="ACHJ48_001112"
     CDS             8595..9266
                     /gene="ygiB"
                     /locus_tag="ACHJ48_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO4706717.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9272..10432
                     /gene="ygiC"
                     /locus_tag="ACHJ48_001113"
     CDS             9272..10432
                     /gene="ygiC"
                     /locus_tag="ACHJ48_001113"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4706718.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(10470..11258)
                     /gene="ygiD"
                     /locus_tag="ACHJ48_001114"
     CDS             complement(10470..11258)
                     /gene="ygiD"
                     /locus_tag="ACHJ48_001114"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO4706719.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            11401..12174
                     /gene="zupT"
                     /locus_tag="ACHJ48_001115"
     CDS             11401..12174
                     /gene="zupT"
                     /locus_tag="ACHJ48_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO4706720.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(12232..12402)
                     /gene="yqiD"
                     /locus_tag="ACHJ48_001116"
     CDS             complement(12232..12402)
                     /gene="yqiD"
                     /locus_tag="ACHJ48_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO4706721.1"
                     /translation="MFIAWYWIVLIVLVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(12664..13317)
                     /gene="ribB"
                     /locus_tag="ACHJ48_001117"
     CDS             complement(12664..13317)
                     /gene="ribB"
                     /locus_tag="ACHJ48_001117"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO4706722.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(13421..13569)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            13691..13990
                     /gene="ubiK"
                     /locus_tag="ACHJ48_001118"
     CDS             13691..13990
                     /gene="ubiK"
                     /locus_tag="ACHJ48_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO4706723.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRSSELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(14025..14225)
                     /gene="glgS"
                     /locus_tag="ACHJ48_001119"
     CDS             complement(14025..14225)
                     /gene="glgS"
                     /locus_tag="ACHJ48_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO4706724.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            14494..15108
                     /gene="yqiJ"
                     /locus_tag="ACHJ48_001120"
     CDS             14494..15108
                     /gene="yqiJ"
                     /locus_tag="ACHJ48_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO4706725.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDHFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNP"
     gene            15150..16811
                     /gene="yqiK"
                     /locus_tag="ACHJ48_001121"
     CDS             15150..16811
                     /gene="yqiK"
                     /locus_tag="ACHJ48_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO4706726.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSLLTPTTPVAENVE"
     gene            17050..17109
                     /locus_tag="ACHJ48_001122"
     CDS             17050..17109
                     /locus_tag="ACHJ48_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4706727.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            17426..17485
                     /gene="ibsE"
                     /locus_tag="ACHJ48_001123"
     CDS             17426..17485
                     /gene="ibsE"
                     /locus_tag="ACHJ48_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO4706728.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(17605..19038)
                     /gene="hldE"
                     /locus_tag="ACHJ48_001124"
     CDS             complement(17605..19038)
                     /gene="hldE"
                     /locus_tag="ACHJ48_001124"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO4706729.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVVA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(19086..21926)
                     /gene="glnE"
                     /locus_tag="ACHJ48_001125"
     CDS             complement(19086..21926)
                     /gene="glnE"
                     /locus_tag="ACHJ48_001125"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO4706730.1"
                     /translation="MKPLSSPLQQYWQTIVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALINVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRWELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLLRITALLAGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(21949..23250)
                     /gene="ygiF"
                     /locus_tag="ACHJ48_001126"
     CDS             complement(21949..23250)
                     /gene="ygiF"
                     /locus_tag="ACHJ48_001126"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO4706731.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            23492..24112
                     /gene="ygiM"
                     /locus_tag="ACHJ48_001127"
     CDS             23492..24112
                     /gene="ygiM"
                     /locus_tag="ACHJ48_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO4706732.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            24176..25414
                     /gene="cca"
                     /locus_tag="ACHJ48_001128"
     CDS             24176..25414
                     /gene="cca"
                     /locus_tag="ACHJ48_001128"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO4706733.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFTTLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(25595..26416)
                     /gene="bacA"
                     /locus_tag="ACHJ48_001129"
     CDS             complement(25595..26416)
                     /gene="bacA"
                     /locus_tag="ACHJ48_001129"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO4706734.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(26507..26875)
                     /gene="folB"
                     /locus_tag="ACHJ48_001130"
     CDS             complement(26507..26875)
                     /gene="folB"
                     /locus_tag="ACHJ48_001130"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO4706735.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DNVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            26981..27598
                     /gene="plsY"
                     /locus_tag="ACHJ48_001131"
     CDS             26981..27598
                     /gene="plsY"
                     /locus_tag="ACHJ48_001131"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO4706736.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(27611..28543)
                     /gene="ttdR"
                     /locus_tag="ACHJ48_001132"
                     /gene_synonym="dan"
     CDS             complement(27611..28543)
                     /gene="ttdR"
                     /locus_tag="ACHJ48_001132"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO4706737.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVYFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            28750..29661
                     /gene="ttdA"
                     /locus_tag="ACHJ48_001133"
     CDS             28750..29661
                     /gene="ttdA"
                     /locus_tag="ACHJ48_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO4706738.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            29658..30263
                     /gene="ttdB"
                     /locus_tag="ACHJ48_001134"
     CDS             29658..30263
                     /gene="ttdB"
                     /locus_tag="ACHJ48_001134"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO4706739.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPLPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            30312..31775
                     /gene="ttdT"
                     /locus_tag="ACHJ48_001135"
     CDS             30312..31775
                     /gene="ttdT"
                     /locus_tag="ACHJ48_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO4706740.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(31818..32831)
                     /gene="tsaD"
                     /locus_tag="ACHJ48_001136"
     CDS             complement(31818..32831)
                     /gene="tsaD"
                     /locus_tag="ACHJ48_001136"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO4706741.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            33069..33284
                     /gene="rpsU"
                     /locus_tag="ACHJ48_001137"
     CDS             33069..33284
                     /gene="rpsU"
                     /locus_tag="ACHJ48_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO4706742.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            33395..35140
                     /gene="dnaG"
                     /locus_tag="ACHJ48_001138"
     CDS             33395..35140
                     /gene="dnaG"
                     /locus_tag="ACHJ48_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO4706743.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            35335..37176
                     /gene="rpoD"
                     /locus_tag="ACHJ48_001139"
     CDS             35335..37176
                     /gene="rpoD"
                     /locus_tag="ACHJ48_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO4706744.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(37255..37761)
                     /gene="mug"
                     /locus_tag="ACHJ48_001140"
     CDS             complement(37255..37761)
                     /gene="mug"
                     /locus_tag="ACHJ48_001140"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO4706745.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDHQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            37886..37961
                     /locus_tag="ACHJ48_001141"
     tRNA            37886..37961
                     /locus_tag="ACHJ48_001141"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37919..37921,aa:Ile,seq:cat)
     gene            complement(38015..38779)
                     /gene="nfeF"
                     /locus_tag="ACHJ48_001142"
                     /gene_synonym="yqjH"
     CDS             complement(38015..38779)
                     /gene="nfeF"
                     /locus_tag="ACHJ48_001142"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO4706746.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            39067..39690
                     /gene="nfeR"
                     /locus_tag="ACHJ48_001143"
                     /gene_synonym="yqjI"
     CDS             39067..39690
                     /gene="nfeR"
                     /locus_tag="ACHJ48_001143"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO4706747.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLNLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(39844..41364)
                     /gene="aer"
                     /locus_tag="ACHJ48_001144"
     CDS             complement(39844..41364)
                     /gene="aer"
                     /locus_tag="ACHJ48_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO4706748.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGGHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            41782..43161
                     /gene="ygjG"
                     /locus_tag="ACHJ48_001145"
     CDS             41782..43161
                     /gene="ygjG"
                     /locus_tag="ACHJ48_001145"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO4706749.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(43203..43535)
                     /gene="ygjH"
                     /locus_tag="ACHJ48_001146"
     CDS             complement(43203..43535)
                     /gene="ygjH"
                     /locus_tag="ACHJ48_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO4706750.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            43754..44737
                     /gene="ebgR"
                     /locus_tag="ACHJ48_001147"
     CDS             43754..44737
                     /gene="ebgR"
                     /locus_tag="ACHJ48_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO4706751.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            44921..48013
                     /gene="ebgA"
                     /locus_tag="ACHJ48_001148"
     CDS             44921..48013
                     /gene="ebgA"
                     /locus_tag="ACHJ48_001148"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO4706752.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAIFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            48010..48459
                     /gene="ebgC"
                     /locus_tag="ACHJ48_001149"
     CDS             48010..48459
                     /gene="ebgC"
                     /locus_tag="ACHJ48_001149"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO4706753.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVDEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            48522..49955
                     /gene="ygjI"
                     /locus_tag="ACHJ48_001150"
     CDS             48522..49955
                     /gene="ygjI"
                     /locus_tag="ACHJ48_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4706754.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT        12264 a        13453 c        12555 g        11736 t
ORIGIN      
        1 tatttctcat ttactactac attaccgcat gattgaatga ttctcaatta catatccgat
       61 ggtaaaataa caaacggata gtgcttagtg agcaacaaga aacaattttc actcacgaca
      121 atccgtgagt ttttgggata acagactaaa tggctaagtt cacaccttca ttctcaggaa
      181 tcaaaggtcg ggcgctcttt tcactgctct ttgcagcacc aatgattcat gcaaccgaca
      241 ctgcaacgac caaagatggc gaaacaatca ctgttacagc ggatgcaaat accgcaactg
      301 aggcgaccga tggttatcaa cctctgagca cttccacggc gacattaacc gatatgccga
      361 tgctggatat cccgcaggtg gtcaatacgg ttagcgatca ggttctggaa aatcagaatg
      421 caacgacgct ggatgaagcg ctttataacg tcagtaacgt ggtacagacc aatacattag
      481 gcggaactca ggacgccttt gtacgtcgtg gttttggtgc taaccgggat ggctccatca
      541 tgaccaacgg cctgcgaact gtacttcctc gcagtttcaa cgccgccaca gaacgtgtgg
      601 aagtgctaaa aggtccggcc tccacgctgt atggcattct cgatcctggt ggattgatta
      661 acgtcgtgac caagcgcccg gaaaaaacat tccatggttc tgtctcagcc acgtcctcca
      721 gttttggcgg aggcactggg caacttgata tcacaggtcc cattgaaggc actcagctgg
      781 cataccgcct gacgggggaa gtgcaggatg aagattactg gcgaaacttc ggtaaagagc
      841 gcagtacatt tattgccccg tcactcacct ggtttggtga taatgcaaca gtaaccatgc
      901 tctattccca tcgggactat aaaactccgt tcgatcgtgg aacgattttc gaccttacga
      961 cgaaacagcc cgtaaacgtt gatcgaaaaa tacgttttga cgaaccgttt aatattacag
     1021 atggtcagtc cgatctggcg caactcaacg cagaatatca tctcaatagc cagtggacag
     1081 cgcgctttga ttacagctac agccaggata aatacagcga caatcaggct cgcgttaccg
     1141 cgtatgatgc aacgacagga acgctgacac ggcgtgttga tgcaactcag ggatctaccc
     1201 agcgtatgca ttctactcgt gcggatctgc aagggaatgt tgatattgct gggttctata
     1261 atgagattct gggtggggtg tcatatgaat attatgatct tctgcgcaca gatatgattc
     1321 gctgtaaaaa cgctaaagat ttcaatatct acaaccccgt ttatggcaat accagcaaat
     1381 gcacaacggt ttcggcgtcg gacagtgatc agacgatcaa acaggagagc tactcagctt
     1441 atgcacagga tgcgctctat ctgaccgata actggattgc cgtcgccggg atccgctatc
     1501 agtattacac gcagtatgcg ggtaaaggcc gtccttttaa tgtcaatact gacagccgcg
     1561 atgaacaatg gacgcccaaa ctggggttag tctacaaact gacgccatcg gtatccttat
     1621 ttgctaatta ttcgcaaaca tttatgccgc aatcgtcaat tgccagctac atcggagatc
     1681 ttccaccgga atcatctaat gcttacgaag tcggggcaaa attcgagctg ttcgatggta
     1741 taaccgcaga tattgcgctg tttgatatcc ataaacgtaa cgtgttgtat accgaaagta
     1801 ttggtgatga aaccatcgcc aaaacggcag gccgcgttcg ttcaagaggg gtagaagtcg
     1861 accttgcggg agcattaact gaaaacatta atatcattgc cagctacggc tataccgatg
     1921 ctaaggttct ggaagatcct gattatgcag ggaaaccatt gccgaatgtt cctcgtcata
     1981 ccggttcgct attcctgacc tatgacattc ataacatgcc aggcaataac acactgacgt
     2041 ttggcggtgg tggacatggc gtaagccgtc gttcggcaac caatggggct gactattatc
     2101 tgccaggcta tttcgttgcc gatgccttcg ccgcatacaa aatgaaattg cagtatccgg
     2161 tcacactgca attaaacgtc aaaaacctgt ttgataaaac gtattacacc tcttccatcg
     2221 ccacaaataa tctggggaat cagattggcg atccgcgtga agtgcaattc acggtgaaaa
     2281 tggaattttg atcaaaaaag cccggtatac acttgtatac cgggctatgt tttattcttc
     2341 tggcgggagg attatcagac ctcaatctcc gccatgtcgc ctttctcttg caaccagttg
     2401 cggcgatctt ccgagcgttt cttcgccagc agcatatcca tcatcgcgtc agtacgctga
     2461 tcgtcttcat catcgatagt cagctgcacc agacggcgag tgttcggatc aagcgtggtt
     2521 tcgcgcaatt gcatcgggtt catttccccc agacctttaa aacgctggac gttcggcttg
     2581 cctttcttgc gttttaattg ctcaagtacg ccctctttct cttcttccgt cagcgcgtaa
     2641 taaacctctt tcccgagatc aatacggtag agcggtggca gtgcgacgta aacgtgaccg
     2701 tgtttcacca acgcgcggaa gtgttttacg aacaaagcgc agagcagtgt agcaatgtgc
     2761 aaaccatcag agtccgcatc cgcgaggata cagattttgc cataacgaag ctggctcaga
     2821 tcgtcgctgt caggatcgat accgatcgct accgaaatat catgcacttc ctgcgaggcc
     2881 agcacttcgt cggaagaaac ttcccaggtg ttaaggatct tacctttcag cggcatgatc
     2941 gcctgatatt cgcgatcgcg cgcctgcttg gcagatccgc ctgcggagtc accttccacg
     3001 aggaacagtt cggtacggtt aaggtcctgc gcggtacaat cagccagttt gccaggcagc
     3061 gccgggccgc tggtcagctt tttacgcacc acttttttag ccgcacgcat acgacgctgg
     3121 gcgctggaaa tcgccatctc tgccagcaac tccgccgcct gaacgttctg gttcagccac
     3181 aggatgaagg catctttcac cacgcccgaa acgaatgccg cacactgacg cgaagagaga
     3241 cgctctttgg tctgcccggc aaactgtgga tcctgcattt ttactgacag cacataggcg
     3301 cagcgatccc agatatcttc cgccgacaac tttacaccgc gcggcaaaat gttgcggtat
     3361 tcacagaact cacgcatcgc gtccagcagg ccctgacgca ggccattaac atgggtaccg
     3421 ccctgcatcg ttgggatcag gttgacgtag ctttcggtca gcagttcacc gccttccggc
     3481 agccacagta gcgcccagtc caccgcttca gtatcgccag cgaaattacc gataaacggt
     3541 ttttctggca gcgtcggtaa accgtttacc gcttccgcca ggtaatcatt cagaccgtcc
     3601 tgatagcacc agcgctgttc ggtgttgttg atctcatctt taaaggtaat ctcaacgccg
     3661 gggcacagta ctgctttggc tttcagcaca tgcgtcaggc gtgaaacaga aaaacgcggg
     3721 ctatcgaaga aggtttcatc cggccagaaa tggacgctgg tgccggtatt gcgtttaccg
     3781 caattgccga caacctgtaa atcctgcacc ttttcgccat tttcaaaggc gatgttataa
     3841 acctgaccat cgcggcgcac gttaacttct acgcgcttcg acagggcgtt aaccaccgaa
     3901 atccccacgc catgcaggcc gccagagaac tggtaatttt tgttagagaa tttaccgcct
     3961 gcatgcagac ggcaaagaat cagttcaacc gccggtacac cctcttccgg gtgaatatcc
     4021 accggcatcc cgcgcccatc gtcaataact tctaacgact ggtcagcatg taaaataacg
     4081 tccacgcgtt ttgcgtgacc cgccagtgct tcatccacac tgttatcaat gacttcttgc
     4141 cccaaatggt tagggcgagt ggtatcggta tacatccccg gacggcggcg aaccggctca
     4201 agcccggtga gtacctcaat ggcatcagcg ttataagttt gcgtcatggt ttaagttagc
     4261 aattcgagtt gatcgtcaga gatggtgcag accaagaaaa tcgacgatcg ggttgaaata
     4321 atcttcgaag cccgtgaatg cgtggttgcc gccttctatg acagtctggc ggcaggaagc
     4381 gtagtacgcc accgcctggc ggtaatccag cacttcatct cccgtctgtt gcagcagcca
     4441 gatcaaatcc ggcgcttcca gcgggtcaat ctgcatgact ttaagatcgt aaatatggcg
     4501 tgactctagc acatattgct gcccggtgta ggggttctcg ttctgaccga gatagtccgt
     4561 cagcagttca aacgggcgca ccgccgggtt taccaccact gcgggcagca taaaacattg
     4621 tgacaaccag gtggcgtaat atccccccag tgacgaaccg acaataccca gcgaatcgcc
     4681 gccatgttcc aggacaatgg attccagcag ctctgccgcg tcggaaggat acggcggcaa
     4741 ctgcggaatg atcatctcaa cgtcagggtg atgttccgcc agccagtttt ttaacaagct
     4801 cgcttttgca gagcgcggcg agctgttgaa accgtgtaaa taaagaagcg tagacatcag
     4861 tagccttctg aagcggtatc aggttggaaa cgtgtgtccg ccaggcgatg cacctcggtg
     4921 gtcagcgtgc catcagcatg taactcgaga gtacgccagc cgggcgcgat agtatccagc
     4981 gtaaagttgg aacagtgcgg cttaaactgc acacaggtcg acggtgttgc cagcaggcgg
     5041 cgaccgttcc agtcgagatc cagctcctga tgaatatgac cgcacagcaa gtatttgacg
     5101 ttcggaaact tcgccagcac ggtatccagt tcgcccgcgt tacgcaggct gtgttgatca
     5161 agccaactac aacccgcagg tagcggatga tgatgcagca gcagcaacgt atggcgctct
     5221 ggcgcatcag ccagtttacg ttccagccac tcaagctgaa actcgcttag ctcaccgtgc
     5281 ggcacgccaa acacctggct atccagcaac aggatttgcc attgctcacc aataaacacc
     5341 cgcttcgccg gggagatacc cgcatcctgt aacgcgctgt acatcgcggg ctggaaatcg
     5401 tggttgcccg gcagccagac gcagggcgca cgaaaacttg cgatgccttc agcgaaatgc
     5461 tgataggccg cagaggattg atcctgcgct aaatcacctg tcgcgacaat caggtcgaat
     5521 tcgtgctggt gtggacgaat cgcctccagc accgcctggt agctttccca ggtgtttacc
     5581 cctaagaggg cttcgtgctt ttgtgcaaac aggtgagtat cggtaatttg taaaatcctg
     5641 actctggcct caccagccag aggaagggtt aacaggcttt ccaaatggtg tccttaggtt
     5701 tcacgacgct aataaaccgg aatcgccatc gctccatgtg ctaaacagta tcgcaaccag
     5761 tccgctaaaa actggttaat ttgatgcttt tcgtcgcgtt gatgcaactt tttattagga
     5821 taatcatacc gcgctttgaa gcgaaaaatc tgctggcttg aacacacttc agccaccatc
     5881 gcgtcatgat acagacgcac cgtcattgac ggaaggctcc agtaactgat cgcgggcgca
     5941 gtctgttcta ttgtcaccag ggtagtgtat cgggtcgatt ccacaatcgt cagccgatat
     6001 tgtgcgtttg ccacctgata gcttacagtt tcgccgggtg cgtcattgcg cggtaacaaa
     6061 cggcgcaatt gtgaaaagtt catctcgcac aggcgcatca tttcaggaaa gtcaggtgtg
     6121 taacgcttca tttatgccca ctcatttttt aacgcttgat gatgcagctg cagccattgc
     6181 aaagcgatga ccgacgctgc gttgtcgatt ttcccctctt ctacccactg gtatgcctgt
     6241 tcccggctta ccacatgaac gcgaatatct tcgttttcat cagccagacc gtgaataccg
     6301 cttgcggtcg tggcgtccac ttcgcccacc ataattgacg aacgctcact ggtgcccccc
     6361 gggcttgcca ggaaacttaa caccggtttg gtccgtttga ctatcagccc cgcctcttca
     6421 atcgcttcgc gacgggcaac atcttccaca ctttcaccct cttcaatcat tccggcaacc
     6481 atctccagta gccaaggggt ttcgctagtg tcgtatgcgg caattcgaat ctgctcaatc
     6541 agcacaactt catcacgcac tgggtcaaag ggtagcaaga ctgcggcgtg accgcgctca
     6601 aaaatttccc gccgtacctc atgactcatt tgcccgttga atagacgatg acgaaatcta
     6661 taaagatcta atgaaaaaaa gccgcgataa agtgtttctc gtgcaataat ttctacatcg
     6721 tttttgccaa atgtaacggg caggttgtct ggcttaagca ttgttaatgt cctggcacta
     6781 atagtgaatt aaatgtgaat ttcagcgacg tttgactgcc gtttgagcag tcatgtgtta
     6841 aattgaggca cattaacgcc ctatggcacg taacgccaac cttttgcggt agcggcttct
     6901 gctagaatcc gcaataattt tacagtttga tcgcgctaaa tactgcttca ccacaaggaa
     6961 tgcaaatgaa gaaattgctc cccattctta tcggcctgag cctttctggg ttcagttcgt
     7021 tgagccaggc cgagaacctg atgcaagttt atcagcaagc acgccttagt aacccggaat
     7081 tgcgtaagtc tgccgccgat cgtgatgctg cctttgaaaa aattaatgaa gcgcgcagtc
     7141 cattactgcc acagctaggt ttaggtgcag attacaccta tagcaacggc taccgcgacg
     7201 cgaacggcat caactctaac gcgaccagtg cgtccctgca gttaactcaa tccatttttg
     7261 atatgtcgaa atggcgtgcg ttaacgctgc aggaaaaagc agcagggatt caggacgtca
     7321 cgtatcagac cgatcagcaa accttgatcc tcaacaccgc gaccgcttat ttcaacgtgt
     7381 tgaatgctat tgacgttctt tcctatacac aggcgcaaaa agaagcgatc taccgtcaat
     7441 tagatcaaac cacccaacgt tttaacgtgg gcctggtagc gatcaccgac gtgcagaacg
     7501 cccgcgcgca gtacgatacc gtgctggcga acgaagtgac cgcacgtaat aaccttgata
     7561 acgcggtaga gcagctgcgc cagatcaccg gtaactacta tccggaactg gcggcgctga
     7621 atgtcgaaaa ctttaaaacc gacaaaccac agccggttaa cgcgctgctg aaagaagccg
     7681 aaaaacgcaa cctgtcgctg ttacaggcac gcttgagcca ggacctggcg cgcgagcaaa
     7741 ttcgccaggc gcaggatggt cacttaccga ctctggattt aacggcttct agcgggattt
     7801 ctgacacctc ttatagcggt tcgaaaaccc gtggtgccgc tggtacccag tatgacgata
     7861 gcaatatggg ccagaacaaa gttggcctga gcttctcgct gccgatttat cagggcggaa
     7921 tggttaactc gcaggtgaaa caggcacagt acaactttgt tggtgccagc gagcaactgg
     7981 aaagcgcgca tcgtagcgtc gtgcagaccg tacgttcctc tttcaacaac attaatgctt
     8041 ctatcagtag tattaacgcc tacaaacaag ccgtagtttc cgctcaaagc tcattagacg
     8101 cgatggaagc gggctactcg gtcggtacgc gtaccattgt tgatgtgttg gatgcgacca
     8161 ccacgctgta caacgccaag caagagctgg cgaatgcgcg ttataactac ctgattaatc
     8221 agctgaatat taagtcagcc ctgggtacgt tgaacgagca ggatctgctg gcactgaaca
     8281 atgcgctgag caaaccggtt tccactaatc cggaaaacgt tgccccgcaa acgccggaac
     8341 agaatgctat tgctgatggt tatgcgcctg atagcccggc acccgtcgtt cagcaaacat
     8401 ccgcacgcac taccaccagt aacggtcata accctttccg taactgatga cgacgacggg
     8461 gcttcggccc cgtctgaacg taaggcaacg taaagatacg ggttatctgc cgcattcttc
     8521 ccccttctcg cttcaatttc gaccagccat cctctattct gatgggtatt taccacaggt
     8581 cccggaagac aaaaatgaaa cggacaaaat ccatacgcca cgcatcgttc cgcaaaaact
     8641 ggagcgcacg ccatctgaca ccagtcgctc tcgcggttgc cactgttttt atgctggctg
     8701 gctgtgaaaa gagtgatgaa acagtgtctc tctatcaaaa tgctgacgac tgttcagctg
     8761 caaacccagg caaaagcgcc gaatgtacca ccgcgtacaa caatgcgctg aaagaagccg
     8821 aacgtactgc gccgaaatac gccacccgtg aagactgtgt tgctgaattt ggtgaaggtc
     8881 agtgccagca ggcaccagcc caggctggca tggcaccaga aaaccaggcg caggcccagc
     8941 aatccagcgg gagtttctgg atgccgctga tggccggtta catgatgggt cgtctgatgg
     9001 gcggcggtgc gggatttgca cagcagccgc tgttctcctc gaaaaaccca gccagcccgg
     9061 cttacggtaa atataccgac gcgacgggta aaaactatgg cgcagcccag ccaggccgca
     9121 ccatgaccgt accgaagaca gccatggcac caaaaccggc gaccaccact accgttaccc
     9181 gtggcggttt tggtgaatct gttgccaaac aaagcactat gcagcgtagc gcaaccggta
     9241 cctcttctcg ttcaatgggt ggctgatacc gatggaaaga gtcagtatta ccgagcgccc
     9301 ggactggcgt gagaaagccc acgaatacgg tttcaatttt cacaccatgt acggcgagcc
     9361 gtactggtgt gaagatgctt actacaagtt gaccctcgcc caggttgaaa agctggaaga
     9421 agtcaccgcc gaactgcacc agatgtgcct gaaagtggtg gaaaaagtga tcgccagcga
     9481 tgagctgatg accaaattcc gcattccaaa acacacctgg agttttgtgc gccagtcatg
     9541 gctgacgcac cagccatctc tttattcgcg tcttgatctg gcgtgggatg gcactggcga
     9601 acctaaactt ctggaaaata acgccgatac gccaacgtca ctatacgagg cggcgttctt
     9661 tcagtggatc tggctggaag atcagcttaa cgccggtaac ttgccggagg gcagcgacca
     9721 gtttaacagt ctgcaagaaa aactgatcga tcgcttcgtt gagctgcgtg aacagtatgg
     9781 cttccagttg ctgcatctca cctgctgtcg cgacacggtg gaagatcgcg gtaccattca
     9841 gtatttgcag gactgcgcga cggaagctga aatcgctacc gagttcctct acattgacga
     9901 tatcgggtta ggtgaaaaag gtcagttcac ggatttacag gatcaggtaa tttccaacct
     9961 gttcaagctg tatccgtggg aatttatgtt gcgtgagatg ttttccacca agctggagga
    10021 tgcaggcgta cgctggctgg aaccggcgtg gaagagcatt atctccaaca aggcacttct
    10081 accgctactg tgggagatgt tcccgaatca cccgaacctg ctgcccgctt attttgcgga
    10141 agatgatcat ccgcaaatgg aaaaatatgt ggttaaaccg atcttctccc gtgaaggcgc
    10201 aaacgtgtcg atcattgaga acggcaaaac cattgaagca gcggaaggtc cgtatggcga
    10261 agaagggatg attgttcagc aattccaccc gttaccgaaa ttcggcgaca gctatatgct
    10321 gattggtagc tggctggtga acgatcaacc cgccggaatt ggcattcgtg aagaccgtgc
    10381 attgatcacc caggatatgt ctcggtttta tccacatatt tttgttgaat aatcaatgat
    10441 gccggatggc attcgccatc cggtaattgt taacctatct gcaccgacag catacttaaa
    10501 ctgcccattt ctataccatc gaccggaatg gtaattggct cctgcccatc ccacgcacct
    10561 aagacataca gcaacggcag atagtgctct ggcgttgggt tcgataacgc gccaccttca
    10621 tggtcgagat aattcaccag aggatgctgc tctaccggcc cttgccacgt cagattcgct
    10681 ttcacatact cattaaacga catcgcccaa ggatacggtg aactatcacc gtgccacttc
    10741 actgtacgca ggttatgcac cacgttgccg ctggcgacca acattattcc ttcatcacgc
    10801 agcgccgcga gttttcgccc catttcgaaa tgccaggcgg caggtttgct actgtcgata
    10861 ctcaactgca ccatcgggat atccgcgtca ggatacatct taatcagcac gccccaggaa
    10921 ccgtggtcaa agccccaggc ttctttatcc agcgtcaccg ggatcggcgc taacagctca
    10981 accagacgct gtgccagcgc aggcgaaccc ggagcaggat aatgcgtatc gtacagcgcc
    11041 tgcgggaagc caccaaagtc atgaatcgtg ggcggcgtct ccatcgcggt cactcctgtt
    11101 ccacgggtaa accagtgagc cgaaactacc acaatcgctt gcgggcgtgg caatgtcatc
    11161 cccaacgtct gccagctgcg ggtatacaaa ttatcttcca gcacgttcat cggactaccg
    11221 tgacctaaaa acaatgctgg catacgtgtt gaagacatga tgatatcctt aactaaaggt
    11281 gtcattttga tatcctcacg atacgcttgt tcggcggagt aagaacccgg ataacaatga
    11341 tgatgatcat cagttatttt gacgatctgc ctgaaggtga agatttataa ggagttgtcg
    11401 atgtcagtac ctctcattct gaccatactg gcgggggcag ccacgtttat tggcgcgttt
    11461 ctcggcgttc tcgggcaaaa accctcgaac cgcttactgg cgttttcgct aggatttgcg
    11521 gcggggatca tgttgctcat ctcattaatg gaaatgcttc ctgccgcact agcagctgaa
    11581 ggaatgtcgc ctgtgttggg ttatggaatg tttatcttcg gtctgcttgg ctattttggc
    11641 ctggaccgca tgttgccgca tgcgcatccg caggatttaa tgcaaaaatc ggtgcagccg
    11701 ttgccaaaat cgatcaagcg cacagccatt ctgctcactc tcggcatcag tctgcataac
    11761 ttcccggaag ggattgccac ctttgtcacg gcgagcagca acctggagct gggatttggc
    11821 atcgcactgg ccgtcgcgtt gcacaatatt cctgaaggtc tggcggttgc aggcccggtt
    11881 tatgcagcaa cgggttctaa acgcacggca attctgtggg cggggatttc tggactggca
    11941 gaaattcttg gtggtgtgct ggcgtggtta atcctcggta gcatgatttc cccggtggtc
    12001 atggcagcaa tcatggcggc ggtcgcagga attatggtgg cgctctcggt tgatgaatta
    12061 atgccgctcg ccaaagagat tgaccctaat aataacccca gctatggcgt actatgtgga
    12121 atgtcagtga tgggattcag tttggtgctg ctacaaacgg cgggaattgg ttaaaagcaa
    12181 catcagaagt tgtccagcgc agggacaact tctgctagtt tcactaaagc cttattctac
    12241 cttttcggct gtctcttctc tcgtactgtt taagtatttg ttccgcgctt caagcagtgc
    12301 gtctctgtcc tggcgaaatg cccgacaata acgtttcaaa tgcaggaaat aacccaccac
    12361 aaccagaaca atcaatacaa tccagtacca ggcgataaac atccttttac ccttaatatt
    12421 taacaatact aaacatttag cgcataaatt tcacatatcc tttttcggat atattcatat
    12481 ggtcgcataa taacggaaca attatatgat tttgatcata atgaaaaaga atgcctgata
    12541 ttaatgaaat tttctcatag aaagagaagc ggaattaaca tcttgcatag tagggaaaaa
    12601 taaaaaccga agcctgctac aacttcggtt tcttgattaa ggcagtaaat taagcagcgg
    12661 ttttcagctg gctttacgct catgtgcctg acggtatgcc accaggtctt caatagtcac
    12721 gagcgccatg ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc
    12781 atcgtcatta gtcagctcac acagcacacc agccggtttg aagcctgcca gcgtcattag
    12841 atcaatagtt gcttcagtat gaccgccacg cgtcagtaca ccaccagcct gagcgcggag
    12901 tgggaaaacg tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc
    12961 gcgaacggtc gtaatacggt cagcggcaga aacaccggta gtcacacctt cagccgcttc
    13021 aatggtcacg gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag
    13081 atcgagttgt ttacggcgat cttcagtaat gcacaggcaa acaataccac taccgtggcg
    13141 aatggtcagc gccatctgct caacagtcat ggtttctgcc gggaagatca tatcaccttc
    13201 gttttcacgg tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcagccag
    13261 tgcattttca acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt
    13321 aaaaaaacct cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat
    13381 atagccaaaa taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg
    13441 actctaaccg tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa
    13501 gcgctcgcgg gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg
    13561 agaataagcg gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata
    13621 attctggttt atgacttacc cttatcgcac tacaatggca ctcaacacct atcagtacag
    13681 ggaaactaca atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc
    13741 aatgcctaaa ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct
    13801 acaagcgcag ctgacgcgtc tcgatctggt aagccgcgaa gaattcgacg ttcagacgca
    13861 agtcctgttg cggactcgtg aaaaactggc gttactggag cagcgcagca gtgaactgga
    13921 agcgcgcaat aattcggtag cagatcttca atctcctccg gctatcccgc ctattgataa
    13981 agcagaataa agcaacacaa aaacgggcca gacggcccgt aatatcagtg ttctaattca
    14041 agttctctgg tttgcatcat ctgttggcaa taccaggcat aacgtgcgca aaaccaggtt
    14101 ctatgttctt catccatgtt accagtaacg gccagaatat cgaccgggcg accttctgcg
    14161 tgcattctgg caaaactacg cgctaggaaa tcgaaattat ttaaagaatt aagactatga
    14221 tccataagca catcctccgg ttatcgcata ccttttaagg ccatatgctt tttctgattt
    14281 cagtattccc caatcagtgt agtgccttta aacataacgt gcgtaaatat attgtccccg
    14341 atcacacttt ttagtgaaaa ataaaaaacc atattaaaag actttctctt ccagaacctc
    14401 tctaacatat gttattatta acaaagacaa atgtaagcaa ggaaactatc acaaagtaaa
    14461 tattgcgtca ctaaatggac attggagtga catatgattt tattcgccga ctataacacc
    14521 ccttatcttt tcgctatttc ctttgtactg ttgatcggcc ttttagaaat ttttgcgctt
    14581 atctgcggtc acatgctctc cggtgctctt gatgcacatc ttgaccatta tgattccata
    14641 accacaggtc atattagcca ggcacttcat tatctcaata tcggaagatt gccagctctc
    14701 gtcgttcttt gcttactggc tggttttttt ggtcttattg gcatcctgtt gcagcatacc
    14761 tgcatcatgg tctggcaatc accactctcg aacctgtttg tggttcctgt cagtttacta
    14821 tttacgatta ttgcggtgca ttataccggt aaaatagttg cgccctggat tccacgcgat
    14881 catagctcag ctattacaga agaagaatat attggtagca tggcgttaat taccggtcat
    14941 caagccactt caggtaatcc ctgcgaagga aaactcaccg atcattttgg ccaaatacat
    15001 tatttactac tggagcctga agaaggaaaa attttcacta aaggagacaa agtgttaatt
    15061 atttgccgac tttctgcaac gcgatatttg gcggaaaaca atccctagcc ccaaatattg
    15121 taatttatta atcaaaagga aatttaataa tggatgatat tgttaattcc gtgccctcct
    15181 ggatgtttac cgcgattatt gccgtatgca ttctgtttat tattggaatt attttcgcca
    15241 ggctctatcg tcgcgcttcg gcagagcaag catttgtccg taccggctta ggggggcaaa
    15301 aagtggtaat gagcggtggc gcaatcgtga tgccgatctt tcatgaaata atccccatca
    15361 atatgaatac tctgaagctg gaagtcagcc gctcaaccat tgatagcctg attacgaaag
    15421 atcgtatgcg cgtcgatgta gtagtcgctt tctttgtgcg ggtaaaacct tcagtagaag
    15481 ggattgccac cgctgcccag acgctggggc aacgcaccct gtcgcctgaa gacttacgta
    15541 tgttggttga agataaattt gtcgatgccc tccgtgcaac agctgcgcaa atgaccatgc
    15601 atgagttaca ggatacccgc gagaacttcg tgcagggagt acaaaatacc gtagctgaag
    15661 atctgtcgaa aaacggcctg gaactggaaa gcgtttcact taccaacttt aaccagacct
    15721 cgaaagaaca tttcaatcct aacaatgcct ttgacgccga aggtttaacc aaactgactc
    15781 aggagacgga gcgccgtcgc cgcgagcgta acgaagttga acaggatgta gaagttgcgg
    15841 tgcgtgagaa aaaccgtgat gcactttcgc gcaagttgga gattgaacaa caagaagcgt
    15901 ttatgacgct tgagcaggag cagcaggtta aaacccgtac cgctgagcag aatgcgaaaa
    15961 ttgcggcttt tgaagctgaa cgtcgtcgtg aagcagagca gacgcgaatt ctggctgaac
    16021 gacagattca ggaaacagaa atcgaccgcg aacaggccgt ccgctcaaga aaagttgaag
    16081 ctgaacgtga agttcgcatt aaagagatcg aacagcagca ggtcaccgaa atcgctaacc
    16141 agacgaaatc gatcgctatt gccgccaaat cggaacaaca gtcccaggca gaagcgcgtg
    16201 ctaaccttgc actcgcagaa gcagtaagcg cccaacaaaa cgtagaaacc actcgccaga
    16261 cagccgaagc cgatcgtgcc aagcaggtgg cgctgattgc cgcagcacag gatgcagaaa
    16321 ccaaagcggt tgaactgacc gtgcgggcga aagcagaaaa agaagccgca gagatgcagg
    16381 cggcggcgat cgttgagtta gccgaagcta cgcgtaaaaa aggcctggcg gaagcagaag
    16441 cacaacgtgc gctgaacgat gctatcaacg tactttctga cgaacaaacc agccttaaat
    16501 tcaaactggc cttgttgcag gcgctacctg cggtaataga gaaatccgtg gaaccaatga
    16561 agtccattga cggtatcaag attattcagg tcgatggtct gaatcgtggt ggtactgcgg
    16621 gggatgcaaa cacgggtaat gtggggggcg gaaacctggc ggaacaagca ttatcagccg
    16681 cactctctta ccgcacacag gcaccactga ttgactcctt gctcaatgaa attggcgttt
    16741 caggcggctc actggctgca ttgacttcac tcttaacccc aacaactccc gtcgccgaaa
    16801 acgtagaata aattttcagc tatgaacatc cctggctcaa aaagccgggg attttttata
    16861 tctgcgttcc gctaaaaggt gcaaatgatc aggctgttgc agcgttttgc gtgattgctc
    16921 ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc atcaacaatc
    16981 ggggagtaag caaagggctg aaacgggaaa gcccctcccg aggaaggggc cttacagaag
    17041 gaaagggtta tgatgaagct cgtcatcata ctgattgtgt tgttactcgt aagtttcgca
    17101 gcttattaac agccaatcag aggggggaga aatcctccct cacccttgtt cctttacttt
    17161 aggttgaaaa aaaatcagcg tcaagaaggt ttcattaaca catatccctg gcccaaaaag
    17221 ccggggattt tttatatctg cgttccgcta aaaggtgcaa atgctcagga cgttgcagcg
    17281 ttttgcgtga ccgctcgggg aaggcaaaac tgcttcaggg aaagcgttgc gcggggtccg
    17341 gcgctcatca acaatcgggg agtcagcaag gggctgaaac gggaaagccc ctcccgagga
    17401 aggggcctta tagaaggaaa gggttatgat gaagctcgtc atcatactgg ttgtgttgtt
    17461 actgttaagt ttcccgactt actaacaact catcagaggg gggagaaatc ctcccttacc
    17521 cttgttcctt tactctaggt tgaaaagaca acagcgtcaa taggcctgcc atgtacgaag
    17581 cgagatctgt gaaccgcttt ccggttagcc ttttttatcc tgttggatct tcttgataat
    17641 gttagtggtc gagcaaccgt cttcaaagtt gagcaccaac acttcgccgc cgttggccca
    17701 gacttcttta ctcccggcga tctcttccgg tttgtagtca ccgcctttca ccagcaggtc
    17761 tggcaggatc ccggcgatca gacgctgcgg cgtgtcttct tcaaacgaca ctacccagtc
    17821 gaccgcttcc agtgcgccca gcacaatcat acgctgttcg agtgggttta ccgggcggga
    17881 atcccctttc agccgtttgg tggaggcatc gctgttgacg gcaacaatca agcggtcacc
    17941 cagcttgcgg gcatttgcca gataagagac gtgcccggca tgtaggatat cgaagacacc
    18001 gttggtcatt accacttttt caccacgttt acgcgctgcc actacagcca gcttcagttc
    18061 ctcttcggtc atcacgccaa agccggtatc tgcgcgtcca cgtacagcgt tttccagctc
    18121 gatcggcgaa accgtggacg tccccagttt gccgaccacc acaccagccg ccgcattggc
    18181 aaagaagcag gcttcttcca gcgaattacc cgctgccagc gttgccgcca ggacgccaat
    18241 caccgtgtcg cccgcaccgg taacgtcata cacttcctgc gcctgagttg gcatatgcag
    18301 cggcgcttta cccggttgca gcagcgacat accctgttcg gaacgggtca ctaacagagc
    18361 cgagagttcg taatcggcaa tcagtttcat gccgcgctca acaatctctt cttcggtctt
    18421 acatttaccg acaacagctt caaattccga gagattcggc gttaacagcg tagcgccgcg
    18481 gtagcgctca aaatcggtac cttttggatc aatcagcacc ggaacacccg ctttacgcgc
    18541 cagttggatc atctgctgta cgcttgccag cgcacctttg gcgtagtcag aaagcaccag
    18601 cgcgccaatc gaactcagcg cctgattaat ccgctcgtgc agcggctgcg gatcaacacc
    18661 ttcgaaacct tcttcaaaat ccagacggat cagctgttgg ttgcgggaaa gtacccgtaa
    18721 tttggtaatg gtcggatgcg tcggtacaga aacgaagtcg catttgacgt tgacgtcggc
    18781 cagagattta ctcagcgcgc gcgctgcatc gtcaatgccc gtcaacccga ccaggcgtgc
    18841 attagcaccg agagaagcga tattcatcgc cacgttagcc gcgccgcccg gacgttcttc
    18901 gatggtattc actttaacca cgggcaccgg cgcttccggc gagatacgac tggaggggcc
    18961 gtaccagtaa cgatccagca tcacatcacc aaccaccatc actcctgcac gttcaaactc
    19021 tggcagcgtt actttcattc ctgtctcctg agagattcaa aatttgcgcg cgataatacc
    19081 atacttcatt cttccaccag ccacttctgc cagcttgccc gcaccagttc acgctctgcg
    19141 gtgaagcaat cctccggcac atggcccggc aattcctgta atgccagatg atgaagttca
    19201 tcgcgaagcg tagtgtaagc acgggtcagc gccatcgctt cctgctcttc cataatgtcg
    19261 ttttgcgcca gtagttccag aatgcgcacg ttgtctgacc agcgcgttaa cttcggtttt
    19321 tcatgagcgt agcgcaacac cagatattgg gtaataaatt cgatatcggt aattccccct
    19381 tcatcagctt tgatatcaaa gcgatcgcga tgtttattgc cgagatgagc gcgcattttc
    19441 tcgcgcattt cccgcacttc cgtttgcaga gttttacctt cacgcggcag cgtcataatc
    19501 tcgcgacgca ctgcgtcaaa gtgcgcggtg agctgcggat cgccgtacac tacacgcgca
    19561 cgcaccagcg cctgatgctc ccacgtccag gcctcgtttt tctgataatc ggcaaatgct
    19621 tctgtggagg taaccagcat tcccgccgcc ccggacgggc gcagccgcgc atccacttcg
    19681 tacaaaatgc cggaagaggt tcgcgtgctg aacaggtgca taatgcgttg cgccagacgc
    19741 agataaaact gtcgtccgtc gatttcccgc tcgccgtcag tcatcgcatc cattgggcaa
    19801 tcatggagga agatcaggtc aagatcggaa ctgtagccta actcccagcc gcccagcttg
    19861 ccgtagccga ccactgcaaa accacgccct tcgcgttcgt tcaggtgatt cggcttaccg
    19921 tagcgggcaa ccatttgaac ccacgcctgc tgaacgacgg catctatcat ggcttccgcc
    19981 agccaggtta agtgatcgct cactttcatc accggtagcg taccggcgat atccgctgcg
    20041 gcgatgcgta acagctgcgc ctgtttgaac tgacgcagcg cctcaagctg ttgctcttcg
    20101 tcatcttccg gcacgcgcag caaatactgg cgcaactcat cgcggtaggc atcggtcgcc
    20161 gtcggctggt aaagggtgtt tggatcgagc aattcatcca gcaataatgg ataacgcgcc
    20221 agctggctgg caatcatcgg cgacgctgca cacagagaaa tcaaatgttt aagcgccgcg
    20281 gggaattcac tgagcaattc taaataggtg gtgcgggtaa caatccccgc cagcaaggcg
    20341 gtaatgcgca acagcgtaac ggcagcgtct tcacgcgcac agacatcact tagcagatgc
    20401 ggcatcagat ggtcgagcac ctgacgcccg cgtggcccga ttgtgcgctt atccagctct
    20461 ttgcggaaat cggcaatcag cgtcagcacc tgtttgcgat cgtcctcgct aagatgcgcc
    20521 agcactggtg tagtgtcatc ttcctgcaac gcatcctgcc acagctcgcg ccactgttcc
    20581 gacagcgact cttcctgagt ttcactttcg tcgtcgccaa tcaattcatt aaacacccgg
    20641 cgcacattgg tcatatgtgc ggtcagcgcc ccggtcaatt gcggccagtc agcaaagtcc
    20701 atcgcccacg ccagccgcgc acggttaagc tcatcagaag gaagcgtctg ggtttgttcg
    20761 tcgttaatgc tttgcagcag gttttccaga cgccgcagga acagatacgc cactcgcaat
    20821 tgttcagcat cgttttcaga aagcagatgc agcgcggcaa tgacgctgag cgttggcagt
    20881 aaagagcgcg actgcagcga cggttcgcgc ccgccgcgaa tgagctggaa cacctgaacg
    20941 ataaattcaa tttcgcgaat gccgcctgcg ccgagtttga tattgtcggt caaaccacgt
    21001 cgacgcactt cacgggcaat cattcctttc atgttgcgca gcgactgaat cacgctgaaa
    21061 tcgatgtaac gacggaaaac aaacgggcgc agcatcgcac gcaactcgtt agcatagacg
    21121 ccttcgctat cgcccataat ccgcgccttg accatcgcgt aacgctccca gtcgcgcccc
    21181 tgctcctggt aataatcttc caacgcggca aagctcagca ccagcgggcc actttcgcca
    21241 aacggacgca gccgcatatc cacgcgatag acgaagccat cctgcgttgg ttgatccagc
    21301 actttaatca gccgctgccc catgcgggta aaaaactgcg cgttatccag ttcccagcgt
    21361 ccaccctgcg tacaaccatg ttccggccag gcaaaaatca gatcgatatc agaggagaaa
    21421 ttcagttccc cgccgcccag cttacccatg cctaaaatca gcagcggttg cgcttcgccc
    21481 tgcgcattgc acggcgttcc ccactcgcgg cagcaggcgt catatagcca gtcacgcgcc
    21541 gcaacaatca gcgtctccgc cagatagctg agctgctgca atatgctctc ttcagtaacc
    21601 agtgccagcg tttgcgccca ggcgatacgt accatgatgc gccgacggaa cagccgcagc
    21661 tcgcgcatta acccggcttc gtcactcaca ttaatgagcg cctcctgcaa ccatgcaacg
    21721 taatgctgcc attcgtcggc ctgcggcggt tggctttcca gttccgtcag ccactctgga
    21781 tgcgcactta tgctgtcctg cacaaaatca ctgaaagtaa gtactgactt cgcctgtgca
    21841 ctaagtgatt cctcggctaa aggctctggc agccgctcaa caatggtctg ccagtactgc
    21901 tgtaacggtg aagagagcgg cttcataagc gattttatcc ttgcctgatt aacgttttcc
    21961 gctgtgcaac cagaacggtt cctgattgtt tgcctcatta cggaaatgtt caatttcgat
    22021 gcgttgcccg gtcgcaatag cgtgatgcag cccctgccag ttctccagcc aggcttgcgc
    22081 gacgacagga tcatagtaac ccgccagcaa cagtattgag tcaatatcac gcgtcaggcg
    22141 tggcaactgg tcacggtagc gatcgcctaa cggctggcag aaaacgcttt tcagttcagc
    22201 ggcatggcgg gaaagatgga tatcggcaaa gcgtttgaag gagtcgctga ttttgccctg
    22261 cgctttggca tctaaaaatg gctgccatgc tttgcttacc aaccattcgg tcaacgccag
    22321 cttcgccatt gccgtttcgg tagagtagac cgccgtcacg gcagaaaccg cagaagcaat
    22381 ggtcgcttcg cattgggtca gcaaatcacg taagtgagtg ctcgctttac gcggcacaat
    22441 accaccgaac agcatcagcg tatgacggac caagctaatg gctgccagca cctgttcttt
    22501 cgccgcatcg ttgccgcgta cccacagttc ttcatgatac tgccattgcg ctaacgccag
    22561 ctcgagcgcc gcttccagcc cctgttccac atcggctttt gccgcaacat gcaaaatggt
    22621 ggtcggtttg atttcacgcg ccggattgcc ctgcgccaga tgatagccac gcgccgcttt
    22681 gctcaggctg ccctggcgta atccggtttg cgataccagt tggttcgcca gtttcagcac
    22741 cgcgcgcgtg tcgccgctaa gcagctccag ttccagctca cagataggtt cagcaaattc
    22801 ccccgctttc acctcaccct gatcgagagc gatttcaatt cggctgccat cgaccgccac
    22861 cagccatttt tcgcgataaa aatcggtgct gaacagcggt tgtacgcggg aagcgagatc
    22921 ggcgggcaat tcgccgttcg gccagacttc cgtcggtaac tgcgccaggt cgagcgtcgg
    22981 ttcgctcaac gccacgttat attccgggcg ctggtgtaag ccgcctgtca ctcttcctgc
    23041 aactttcatg gtcatctcat agcgaccgtt ttcgccacga atacgtaagc ccatatcgtg
    23101 cccacgcagc cagttatccg gcgtttcgta gtaaatattc agtaactgca cggggtcatg
    23161 gtgctcgccg cccagcgtat tgagatggtc acgcaacgcc tcaacggcgc tgtgattaac
    23221 aataaacttt aattcgattt cctgagccat ggcctttact tatcggttat gtcacataag
    23281 tgacgatgaa cggcgaactt aatgcgatct tttttgtcag tagatagtat tttgcgccaa
    23341 attgccatgc aacgggcaat ttgacgggcg taaaagtttg aagcagtggc aagacctgac
    23401 acagaggatt ccgattgatg tcgaatgctt tgcgtagcga cactgatgcc actactatcg
    23461 ttccactttc catgacaata acgacagcct gatgccaaaa ttacgcctga tcggattaac
    23521 tttactcgca cttagcgcga ctgccgtctc acacgctgaa gaaacgcgct atgtttctga
    23581 cgaactgaat acctgggtcc gtagcggtcc gggagatcat tatcgcctcg tgggcacggt
    23641 taacgccggc gaggaagtga ccttattaca aactgacgcc aacaccaatt atgcccaggt
    23701 gaaagacagc tctggccgta ccgcctggat cccgttgaaa caacttagca ctgagccaag
    23761 cctgcgctcc cgtgtgccag atctggaaaa tcaggtcaaa accctgaccg ataaactcac
    23821 caatatcgat aacacctgga atcagcgcac cgcagaaatg cagcaaaaag tggcgcagag
    23881 cgacagcgtg atcaacgggt taaaagaaga aaatcagaaa ctgaaaaacg agctgattgt
    23941 cgcgcagaaa aaggtcgatg ccgccagcgt gcagctggat gacaaacagc gcaccatcat
    24001 catgcaatgg tttatgtatg gcggcggcgt gctggggctt ggcttgctgc tcggtctggt
    24061 actgccgcac ctgatcccaa gccgcaaacg caaagatcgc tggatgaact aaatcgcctt
    24121 cttcgttgca ctgtcttatt attaggaatt gtgtatttga cgaagggatg atggcgtgaa
    24181 gatttatctg gtcggtggtg ctgttcggga tgcattgtta gggctaccgg tcaaagacag
    24241 agattgggta gtggtcggca gtacgccaca ggagatgctc gacgcgggct accagcaggt
    24301 aggccgcgat tttcctgtgt ttctgcatcc gcaaacgcat gaagagtatg cgctggcacg
    24361 taccgaacgg aaatccggct ccggttacac cggttttacc tgctatgccg caccggatgt
    24421 cacgctggaa gatgatctta agcgtcgcga tctgaccatt aatgcgctgg cccaggacga
    24481 taacggcgag attatcgacc cgtacaacgg tctgggcgat ctgcaaaatc gtctgttgcg
    24541 ccatgtttcc cccgcttttg gcgaagatcc gttacgcgta ctgcgcgtgg cgcgttttgc
    24601 tgcgcgttat gcccacctcg gttttcgtat tgccgatgaa actctgacgc tgatgcgcga
    24661 gatgacccat gcgggtgaac tggaacacct gacgcctgaa cgggtatgga aagagacaga
    24721 aaacgccctc accacccgca atccacaggt gttcttccag gtactgcgcg attgcggcgc
    24781 actgcgcgtt ttattcccgg aaattgacgc cctgtttggt gttccggccc ctgccaggtg
    24841 gcatccggaa atcgacacgg gtattcatac cttaatgacg ctctcaatgg cggcgatgct
    24901 gagtccgcag gtcgatgtcc gtttcacaac tttatgccac gatctcggta aaggactgac
    24961 gccgccagaa ctctggccgc gtcatcatgg tcatggcccg gcgggtgtta agttagtgga
    25021 acaactatgc cagcgtctgc gcgtgccaaa tgaaattcgc gatttagcca gactggtggc
    25081 tgagtttcac gatctcatcc acaccttccc aatgctgaac ccaaaaacca tcgtcaaatt
    25141 atttgattcc atcgatgcct ggcgtaaacc gcagcgtgtc gagcaactgg cgctgaccag
    25201 cgaggctgac gtgcgcggca gaaccggttt tgaatcagcg gactacccgc aaggccgctg
    25261 gttgcgcgaa gcctgggaag tggcgcagtc agtgccgaca aaagccgtcg ttgaagcggg
    25321 atttaaaggt gtggagattc gcgaggagct gacccgacgg cggattgcgg cggtagccag
    25381 ctggaaggaa caacgttgcc caaagcctga gtgagtattg ggcattttgc cggatgcggc
    25441 gtaaacgcct tatccggcta catgtcaacg acagttgtag gcctgataag acgcgcaagc
    25501 gtcgcatcaa gcgttgattg ccggacgcgg cgtgaacgcc ttattcggcc tacgcaatcg
    25561 tggacaacgc caggcatccg acactattcc tcaattaaaa gaacacgaca tacaccgcag
    25621 ccgccacaat aaagcgataa atggcgaacg ggataaacga aatgcgctta atcaattgca
    25681 ggaaggtttt aatcgctatc agcgccacca caaaagcggt gataaaccca acggcaaaca
    25741 tcgggatatc gccgcttgtc aggaagcccc agcttttgta gagatcgagc gccgttgcgc
    25801 ccatcatcat cggcaccgcc agcaggaacg aaaactcgga agcagcgtaa cggctcaccc
    25861 ccatcagcat cccacctgaa atggtcgccc cggaacggga gaaacccggc cacagcgcca
    25921 gacactggaa acaaccaatc ataaacgcct gacgataggt catatcatca agacccggcg
    25981 cacgcggctc tttcggcttc aggcattcgg cggcaatcag caacaaaccg ccaacgacca
    26041 gcgcatacat cacatttatc gggttaaaca atgacttaat cgtgtcgtgg aacaacagcc
    26101 ccagtaccac cgccggaatc atccccagca aaatgtggat cagcgttaaa cgacctttgc
    26161 tttctccttc gtgctgcaac gggcggccaa agtggatgcc aatcaggcca aacagacgcc
    26221 gccagaacat cactactacc gccagaattg atcctaactg gatcacaact tcaaaggttt
    26281 tcgccgtgtc gccctcaaac cccaacaagt gaccgacaat aatcatatgg cccgtgctgg
    26341 ataccggcag aaattctgtc aatccttcga ccacacccaa tattgccgct atcagcagcg
    26401 agtgcatatc gctcatcaat aaacccctaa attataaaaa tgtaacgctt gtccgaacta
    26461 ctgcagtatg accaggttat aaccgtttgg tttaacagct gtaaaattaa ttattttctt
    26521 tcagattatt gccacgctca atgattacgc caacattcgc cgcccgcgcc actgcgcctg
    26581 gcttgctgag tttgatacgc acccacggcg agttgaagcg tgctaacagc agctctgcca
    26641 cctcttccgc cacgcgctcc accagcgcaa aacgtgcccc ctcgacgtgg ctgaccaccg
    26701 tttctgcaat gtcagcgtag ctgaggcaat ccgccacatt atcgcttttt gccgctttac
    26761 ggttatccca cgccatttcg atatcgaaca ctaatttctg ttcgatggtc tgttcccagt
    26821 cgtaaacacc aatagtggtg attaccgaaa gttgctctat aaatacaata tccatcacga
    26881 cctgctcgct ttttggctaa cccggatacc acttccggcg aaatgtgcgt attatccaca
    26941 gattcatcgt ttaacacgaa ttttcaaaac ggaacagctt atgagtgcaa tcgcgcctgg
    27001 aatgatcctc atcgcgtacc tctgcggctc catttccagt gccattctgg tttgccgcct
    27061 gtgtgggctg cccgatccgc gaaccagcgg ctccggtaat cctggcgcaa ctaacgtgtt
    27121 acgtataggt ggcaaaggag cagccgtagc agtactgatt ttcgacgttc tgaaaggaat
    27181 gttgcccgtc tggggcgcgt atgaattagg tgtcagcccc ttctggctag gcttaattgc
    27241 catcgccgcc tgtcttggac acatctggcc cgttttcttc ggatttaaag gaggaaaagg
    27301 cgttgctacc gcttttggtg ccatcgcacc cattggctgg gatctcaccg gagtaatggc
    27361 gggaacctgg ttactgaccg tgctattgag cggatactcg tcgctgggag cgattgtcag
    27421 tgcactgatt gctccgtttt atgtctggtg gtttaagcca caattcacct tcccggtttc
    27481 gatgctctct tgcctgatcc tgctgcgtca tcatgacaac atccagcgtc tgtggcgtcg
    27541 tcaggagaca aaaatctgga cgaaattcaa aagaaagcgc gaaaaggatc ccgagtgatt
    27601 tctggtggat ctacatgacc tgatagcctt catcgggttt gcccagccgt tgctggcacc
    27661 atgccgccag aaattccacg cagacacgta atttcatgct gcgatagagc ggctcccggt
    27721 aaacagccca gatattggcg ctttgtgcat actccggcaa tacttgcacc agtttgccac
    27781 tctccagaaa cggcaacaca tcccactcgg aacgcagcat aatccctttg ccctccagcg
    27841 cccattgcag cacaatctcg ccgctattgg aggaaagatg cccgctaacc ttcaccgatt
    27901 ttttctcctg accgttcccc aactcccata ttccgtgggt catatcgcgc tctttggtca
    27961 ccagacagtc atgacgactt aattcctgta gggattgtgg ctgcggatat ttttgcagat
    28021 actcaggtgc tgcgcataat attcttttat ttttcgttaa cagatgcgca atataataat
    28081 caggaatttc gtcattaata cgaatatcca gatcaatatt atcctgcact aaatcaattt
    28141 gccgatcgaa caattcaaaa tacacctgta actcaggata attgcgcata agttcggtaa
    28201 tggctggcgc aatatggctg cgcccaaaac caaaactgca accaatacga atcatcccct
    28261 ccgggcgcgt tttgatttgc gtgacgtcat cgaccagtcg ctgatattgg gtgaggattt
    28321 ccagcgcatg ttcgtaacaa cgttggccac tttcagtcag cgccacgccg cgagcggagc
    28381 ggttcagcag cgttgttgcc agcgtatttt caaggatttg gatccgcttg gtgacaaatg
    28441 caggcgtttg cccaagcgtc gccgctgcag cactaaaact cccgctgtgg acgatttcta
    28501 ccagtacctg caagtctttg gctaaaggcc agctattcag catcgttaat tatccgcagt
    28561 tgtgataagc gcagtgtatt tcgcaaaaca ttgccattcc ctgctctggc gcaatattca
    28621 ttcttatttt cgcgttgatt cacgaactgt taattacata ttcacggtgg caaaaaatat
    28681 aaaaccacat ttttagtggt agtttgtggc ggtgaatttt tccagacaaa tacaaaaact
    28741 ggagttgcca tgatgagcga aagtaataag caacaggcag tgaataagtt gacggagatt
    28801 gtcgctaact ttaccgccat gatttctacc cgaatgcctg atgacgtggt ggataaacta
    28861 aaacagctaa aggatgccga aacgtcgtcg atggggaaaa ttatctacca tacgatgttc
    28921 gacaacatgc aaaaagcgat tgacctgaat cgtcctgcct gtcaggacac cggggagatt
    28981 atgttcttcg ttaaagtcgg ttcccgcttc ccactgcttg gcgagctgca aagcatactc
    29041 aaacaagccg tggaagaggc gaccatcaaa gcgccgctgc gtcacaatgc ggtagaaatt
    29101 tttgacgaag taaacaccgg caaaaatacc ggtagcggcg taccgtgggt cacctgggat
    29161 atcgtccccg acggtgacga tgcggaaatc gaagtttaca tggcaggcgg cggctgcacg
    29221 ctaccaggcc gctcgaaagt gttaatgccg tcagaaggct acgaaggcgt ggtgaaattc
    29281 gtcttcgaaa atatctccac cctcgcagta aacgcctgtc caccggtact ggtgggcgtg
    29341 ggcatcgcca cctcggtgga aaccgccgcc gtactctcgc gtaaagccat tttgcgcccg
    29401 attggcagcc gccaccccaa tccaaaagcg gcagagctgg agctacgcct ggaagaagga
    29461 ctcaaccgtc tggggattgg tccacaaggg ctgactggca acagttcagt gatgggcgtg
    29521 catatcgaat ctgccgcccg ccatccgtca accatcggcg ttgctgtttc taccggttgc
    29581 tgggcgcatc gtcgcggcac actgctggtt catgccgatc tcacctttga aaatctgtct
    29641 cacacccgga gcgcgttatg aaaaagatcc tgacaacccc gatcaaagct gaagatctgc
    29701 aagatattca cgtcggcgat gtgatctatc tgaccggtac gctggtgacc tgccgtgacg
    29761 tttgtcaccg ccgattgatc gaactgaaac gtccgctccc ttacgatctc aacggcaaag
    29821 cgattttcca cgctggcccc atcgtgcgca aaaacggcga caaatgggag atggtctccg
    29881 tcggtccgac aaccagtatg cgtatggaaa gttttgaacg tgaatttatc gagcagaccg
    29941 gcgtgaaact ggtggtcggc aaaggcggaa tggggccgct gaccgaagaa ggctgtcaga
    30001 aattcaaggc gctacatgta attttcccgg caggctgcgc ggtgctggcg gcaacccagg
    30061 tggaagagat tgaagaagtg cactggacag aactcgggat gccggagtca ctgtgggtct
    30121 gccgggtcaa agagttcggc ccgctgattg tctctattga tacccacggc aacaacctga
    30181 tagccgaaaa caaaaagctg ttcgccgaac gccgcgatcc catcgtggaa gagatctgcg
    30241 agcacgtcca ttacatcaaa taaccctccc ggagaggctc gccccctctc ctttttcgca
    30301 ggcataacac gatgaaacct tccactgaat ggtggcgata ccttgcgccg ctggcggtca
    30361 tcgccattat tgctctaatt ccggttcccg cagggctgga gagtcatacc tggctctact
    30421 ttgccgtttt tactggcgta atcgttggac tgatcctcga acccgtgccg ggtgccgtgg
    30481 tggcgatggt gggtatctcc atcatcgcca tactctctcc ctggctgctg ttcagcccgg
    30541 agcagctcgc tcagccaggc tttaaattca ctgcaaaatc cctctcgtgg gccgtttccg
    30601 gtttttctaa ttcggttatc tggctgattt tcgccgcctt tatgtttggc acaggctatg
    30661 aaaaaaccgg gctggggcga cgtatcgcgc tgattctggt gaaaaagatg gggcatcgca
    30721 cgctgtttct tggctatgcg gtgatgttct ccgagctgat cctggcacct gtcacaccgt
    30781 ccaactcggc gcgtggtgcg gggattatct atcccatcat ccgtaacctg ccgccgctct
    30841 atcaatcaca accaaacgac agcagttcgc gcagtattgg ctcgtacatc atgtggatgg
    30901 ggattgttgc cgactgcgtg accagcgcca ttttcctgac ggcgatggct cctaacttgc
    30961 tgttaattgg actgatgaaa agcgcatctc acgccacgct gagttggggc gactggttcc
    31021 tcgggatgtt gccgctcagt attttactgg tcctgctggt tccctggctg gcttacgtgc
    31081 tgtacccgcc tgtactgaag tcaggcgatc aggtgccgcg ctgggcagag acggaactgc
    31141 aggcaatggg cccgctctgt tcgcgtgaaa aacggatgct ggggctgatg gtaggcgcgc
    31201 tggtgctgtg gattttcggc ggtgattata tcgatgccgc gatggtcggt tacagcgtgg
    31261 tggcgctgat gctgcttctg cgcattatca gttgggacga tattgtcagt aataaagcgg
    31321 cgtggaacgt tttcttctgg ctggcctcgc ttatcaccct cgcgaccgga ctcaacaaca
    31381 ccggttttat tagctggttt ggcaaactgt tagcaggcag cttaagcggt tattcgccaa
    31441 cgatggtgat ggtggcgttg attgtggtgt tttatctact gcgctacttt tttgccagcg
    31501 ccacggcgta tacctccgct ctcgcaccga tgatgattgc cgctgcgctg gcgatgccgg
    31561 aaatcccgct gccggtgttc tgcctgatgg ttggtgcggc aattggtctg gggagcattc
    31621 ttacaccata cgccaccggc cccagtccga tttactacgg cagtggttat ctgccaacgg
    31681 cggattactg gcgactgggg gcgatttttg ggctgatatt cctcgtattg ctggtgatta
    31741 ccggcttact gtggatgccc gtggtgttgc tttaagtaag gacggtgccg gaagtaactc
    31801 tccggcaccc gatagtttta cgcagccggt aactccgcca gcggccagcg cggacgcacg
    31861 ctaacgccga gatccgccgt cgcgcctgct ttaaaccgca ccattccggc ataggcgatc
    31921 atcgcgccgt tatcagtaca aaattccgga cgcgcgtaga acacttcgcc gcggcgtttt
    31981 ttcatcattt cagccagctt cgcccgtaac gtgcggttag cactcacgcc gcccgccatg
    32041 accagtcgct taaagcccgt ctgatccagc gcacgcttac acttaatcat caacgtatcg
    32101 accaccgcat cttcaaaggc gcgggcgatg tcagcacgcg tctggtcgtc ggtgccgttg
    32161 tcacgaatgg tatttgccgc gaaggttttc aggccggaga agctgaaatc cagccccgga
    32221 cggtcggtca tcggacgcgg gaaaacaaag cgcccggcag taccctgcgc cgccattttc
    32281 gacagtaacg gtccgccagg ataatccagc cccagcagct tcgcggtttt atcaaacgct
    32341 tcaccggcgg catcatcgat agactcgccg agcagctcgt actgaccaat gccagtcacg
    32401 ctgattaact gcgtatgacc gccggaaacc agcagcgcaa caaacggaaa ttccggcggg
    32461 ttatcttcca gcatcggcgc taacagatgc ccttccatat ggtgtacagg gatcgccgga
    32521 acgttccagg caaacgccag agaacgcccc acggtcgcgc caaccagcag tgcgccgact
    32581 aatccagggc ctgcggtata ggccacagca tcaatgtctt ttgccgttaa accagactcc
    32641 tttagcgccg cctggatcaa cggtacggtt ttacgcacat gatcgcggga ggccagttca
    32701 ggcacaacgc ccccgtagtc agcgtgcaat ttcacctgac tatacaattg gttggctaac
    32761 aaaccttttt catcgtcgta aatggcgatg ccggtttcat cgcaggaagt ttcaataccc
    32821 agtacacgca tgacttgttt tacctcgctt tattaccgcg cagtgtagga ccaatgcggg
    32881 ttgatgtaaa actttgttcg cccctggaga aagcctcgtg tatactcctc acccttataa
    32941 aagtcccttt caaaaaaggc cgcggtgctt tacaaagcag cagcaattgc agtaaaattc
    33001 cgcaccattt tgaaataagc tggcgttgat gccagcggca aaccgaatta atcaaaggtg
    33061 agaggcacat gccggtaatt aaagtacgtg aaaacgagcc gttcgacgta gctctgcgtc
    33121 gcttcaagcg ttcctgcgaa aaagcaggtg ttctggcgga agttcgtcgt cgtgagttct
    33181 atgaaaaacc gactaccgaa cgtaagcgcg ctaaagcttc tgcagtgaaa cgtcacgcga
    33241 agaaactggc tcgcgaaaac gcacgccgca ctcgtctgta ctaattcccc gagagcgttg
    33301 ctctccgatc agaccgagtt gtagttgtaa ggccgtgctt ccgaaaggaa tgcgcggctt
    33361 attttcgttt atgaattgct aaaaatcggg gcctatggct ggacgaatcc cacgcgtatt
    33421 cattaatgat ctgctggcac gcactgacat cgtcgatctg atcgatgccc gtgtgaagct
    33481 gaaaaagcag ggcaagaatt tccacgcgtg ttgtccattc cacaacgaga aaaccccgtc
    33541 cttcaccgtt aacggtgaga aacagtttta ccactgcttt ggatgtggcg cgcacggcaa
    33601 cgcgatcgac ttcctgatga actacgacaa gcttgagttc gtcgaaacgg tcgaagagct
    33661 ggcggcaatg cacaatcttg aagtgccatt tgaagcaggt agcggcccca gccagatcga
    33721 gcgccatcag cggcaaacgc tttatcagtt gatggacggt ctgaatacgt tttaccaaca
    33781 atctttacaa caacctgttg ccacgtctgc gcgccagtat ctggaaaaac gcggattaag
    33841 ccacgaggtt atcgctcgct ttgcgattgg ttttgcgcct cccggctggg acaacgtcct
    33901 gaagcggttt ggcggcaatc cagaaaatcg ccagtcattg attgatgcgg ggatgttggt
    33961 cactaacgat cagggacgca gttacgatcg tttccgcgag cgggtgatgt tccccattcg
    34021 cgataaacgc ggtcgggtga ttggttttgg cgggcgtgtg ctgggcaacg atacccccaa
    34081 atacctgaac tcgccggaaa cagacatttt ccataaaggc cgccagcttt acggtcttta
    34141 tgaagcgcag caggataacg ctgaacccaa tcgtctgctt gtggtcgaag gctatatgga
    34201 cgtggtggcg ctggcgcaat acggcattaa ttacgccgtt gcgtcgttag gtacgtcaac
    34261 caccgccgat cacatacaac tgttgttccg cgcgaccaac aatgtcattt gctgttatga
    34321 cggcgaccgt gcaggccgtg atgccgcatg gcgagcgctg gaaacggcgc tgccttacat
    34381 gacagacggc cgtcagctac gctttatgtt tttgcctgat ggcgaagacc ctgacacgct
    34441 ggtacgaaaa gaaggtaaag aagcgtttga agcgcggatg gagcaggcga tgccgctttc
    34501 cgcatttctg tttaacagcc tgatgccgca agttgatctg agtacccctg acgggcgcgc
    34561 acgtttgagt acgctggcac tgccattgat atcgcaagtg ccgggcgaaa cgctgcgaat
    34621 atatcttcgt caggaattag gcaacaaatt aggcatactt gatgacagcc agcttgaacg
    34681 attaatgcca aaagcggcag agagcggcgt ttctcgccct gttccgcagc taaaacgcac
    34741 gaccatgcgt atacttatag ggttgctggt gcaaaatcca gaattagcga cgttggtccc
    34801 gccgcttgag aatctggatg aaaataagct ccctggactt ggcttattca gagaactggt
    34861 caacacttgt ctctcccagc caggtctgac caccgggcaa cttttagagc actatcgtgg
    34921 tacaaataat gctgccaccc ttgaaaaact gtcgatgtgg gacgatatag cagataagaa
    34981 tattgctgag caaaccttca ccgactcact caaccatatg tttgattcgc tgcttgaact
    35041 gcgccaggaa gagttaatcg ctcgtgagcg cacgcatggt ttaagcaacg aagaacgcct
    35101 ggagctctgg acattaaacc aggagctggc gaaaaagtga tttaacggct taagtgccga
    35161 agagcgatcg ggaagccccc gacagccgca ctgagaggca gcggcaaata tataagtacg
    35221 ccctcgtaat tatcgttggc ggtaaacaac cgttggattt cagcgttaac ggctgaagga
    35281 catcgggtca atcgcccaac accaacctca tgaaataagt gtggataccg tcttatggag
    35341 caaaacccgc agtcacagct gaaacttctt gtcacccgtg gtaaggagca aggctatctg
    35401 acctatgccg aggtcaatga ccatctgccg gaagatatcg tcgattcaga tcagatcgaa
    35461 gacatcatcc aaatgatcaa cgacatgggc attcaggtga tggaagaagc accggatgcc
    35521 gatgatctga tgctggctga aaacaccgcg gacgaagatg ctgccgaagc cgccgcgcag
    35581 gtgctttcca gcgtggaatc tgaaatcggg cgcacgactg acccggtacg catgtacatg
    35641 cgtgaaatgg gcaccgttga actgttgacc cgcgaaggcg aaattgacat cgctaagcgt
    35701 attgaagacg ggatcaacca ggttcaatgc tccgttgctg aatatccgga agcgatcacc
    35761 tatctgctgg aacagtacga tcgtgttgaa gcagaagaag cgcgtctgtc cgatctgatc
    35821 accggctttg ttgacccgaa cgcagaagaa gatctggcac ctaccgccac tcacgtcggt
    35881 tctgagcttt cccaggaaga tctggacgat gacgaagatg aagacgaaga agatggcgat
    35941 gacgacagcg ccgatgatga caacagcatc gacccggaac tggctcgcga aaaatttgcg
    36001 gaactgcgcg ctcagtacgt tgtaacgcgt gacaccatca aagcgaaagg tcgcagtcac
    36061 gctgccgctc aggaagagat cctgaaactg tctgaagtat tcaaacagtt ccgcctggtg
    36121 ccgaagcagt ttgactacct ggtcaacagt atgcgcgtca tgatggaccg cgttcgtacg
    36181 caagaacgtc tgatcatgaa gctctgcgtt gagcagtgca aaatgccgaa gaaaaacttc
    36241 attactctgt ttaccggcaa cgaaaccagc gatacctggt tcaacgcggc aattgcgatg
    36301 aacaagccgt ggtcggaaaa actgcacgat gtctctgaag aagtgcatcg cgccctgcag
    36361 aaactgcagc agattgaaga agaaaccggc ctgaccatcg agcaggttaa agatatcaac
    36421 cgtcgtatgt ccatcggtga agcgaaagcc cgccgtgcga agaaagagat ggttgaagcg
    36481 aacttacgtc tggttatttc tatcgccaag aaatacacca accgtggctt gcagttcctt
    36541 gacctgattc aggaaggcaa catcggtctg atgaaagcgg ttgataaatt cgaataccgc
    36601 cgtggttaca agttctccac ctacgcaacc tggtggatcc gtcaggcgat cacccgctct
    36661 atcgcggatc aggcgcgcac catccgtatt ccggtgcata tgattgagac tatcaacaaa
    36721 ctcaaccgta tttctcgcca gatgctgcaa gagatgggcc gtgaaccgac gccggaagaa
    36781 ctggctgaac gtatgctgat gccggaagac aagatccgca aagtgctgaa gatcgccaaa
    36841 gagccaatct ccatggaaac gccgatcggt gatgatgaag attcgcatct gggggatttc
    36901 atcgaggata ccaccctcga gctgccgctg gattctgcga ccaccgaaag cctgcgtgcg
    36961 gcaacacacg acgtgctggc tggcctgacc gcgcgtgaag cgaaagttct gcgtatgcgt
    37021 ttcggtatcg atatgaacac cgatcacacg ctggaagaag tgggtaaaca gttcgacgtt
    37081 acccgtgaac gtatccgtca gatcgaagcg aaggcgctgc gcaaactgcg tcacccgagc
    37141 cgttctgaag tgctgcgtag cttcctggac gattaatcgg taggccggat caggcgttac
    37201 gccgcacccg gcacctgtcc ctttgcacaa acgccacctt ttcggtggcg ttttttatcg
    37261 cccacgcacc accagcgcct ggtccagctc gcgatacgct tcaaccagtt tctccagtga
    37321 aacgcgactt aaaccgctgg gatttggcag cacccaaatc tgcgtcgaac caatgctgag
    37381 cgtttgtttc ccccactgtg caccacgctg gctgaatccc tgttcatatg cttgtttgcc
    37441 cagaatcgcc aacgcttgcg gctgataatc ttcaattttt tcaatcagct tacgcccacc
    37501 tgcgtgtagc tcttgctttg aaatttcatt ggcttgcacc gttggcctgt ctaccagttt
    37561 ggtgacgcca caacgataat ccagcagatg ctgtgcctcc tgcggcttca actgatggtc
    37621 ggtaaacccg gcctgatata tcaccttcca gaagcgattt gccggatgag caaagggaaa
    37681 accagtcccg gcggatgaaa gcccagggtt gataccgcaa aacacgaccc gtaaccctgg
    37741 agccaaaata tcctcaacca tatttactcc tttctaacat caccaggaaa agtataacgg
    37801 attgaaaata cattgtttat aaaaacagca ggcgcgcggt aatggctgga ttgcgacacg
    37861 gagttacttt ataatccgct accatggccc cttagctcag tggttagagc aggcgactca
    37921 taatcgcttg gtcgctggtt caagtccagc aggggccacc agatatagca aaggctgacg
    37981 agaaatcgtc agcctttttc tttttatata tcagttactt tgcgtgccag taggccgctg
    38041 cacgtacccg ctgtgggtca tactgttccg cttcaaagcg gcggcttaaa ttcttaacga
    38101 ctttaccttc gccggttatc cagatgaagt aatcatcggc agggatttgc atctgcgcca
    38161 gacgcgcatc taccgcctgc tcatcatgtg ccagccattc gatattaaaa ccatcaaggt
    38221 gcgcgagata atcctgacag gcgttatccc gcacgctaac cagcgcacta acttgcggtt
    38281 taacggcaag tttgctcaac gtttccaggc ggcggcgcaa tgcgggcatt ccggattcat
    38341 cgcagacata cagctgatac gcgtaatctt ccggcaccac cagcgaaccg cgcggacctg
    38401 ccaccgtaag tttatcgccc ggttgcgcct gcatcgccca gccgctggcg accccaccgt
    38461 cgtgaataaa gaaatcgatc gccagttcat ggcgtagttc gtcatacagc ggcgtatagt
    38521 cacgcgacgg tgggcgtggt ccttccggcc agacgatgcc ctcttccgtt accgttggcg
    38581 gcacaaagtg agcgtcaggt tgaggaaaga agagtttgct gtgatcgtca aagccgcgcg
    38641 atgtaaaacc gtccagcgcc tcgccgccaa ggacaatgcg ctgaaaaccg gcgctgatac
    38701 gctcaacgcg tagcacagtc aattcacgga agcgcagatc attgcgaacg cgctgcgggt
    38761 agcgggggga gttattcatt gttatcgcct tcgtgatggt aatcagatat atctaaataa
    38821 ttttcgcaaa tgataatgat tgttaatcat gataaatgca agcgatttgt agaactgata
    38881 tgtctatagt ctgacaagac gaaccgcctc ttctcaggca tcattactca acgccggatg
    38941 cggcgtgaac gccttatccg gcctacgtgt gagatgagtc cacatttaaa aatcattttt
    39001 attcttgcaa gagtgctcac atcagatata aattagatat atctaattaa gcaaaaggag
    39061 gctgatatga gccatcatca cgaagggtgt tgtaaacatg aaggccagcc acgccatgag
    39121 ggctgctgca aaggtgagaa gtcagaacac gagcactgcg gacacggtca ccagcatgaa
    39181 cacggtcaat gttgcggtgg tcgccacggt cgcggcggcg gtcgtcggca acgtttcttt
    39241 ggtcacggtg aattgcgtct ggtgattctg gatattctct cgcgcgatga cagccacggt
    39301 tacgaattga ttaaagcgat tgagaatcta acccaaggga attacacccc aagcccgggc
    39361 gtcatctacc cgacgctgga ttttctgcag gagcagtcgc tgattaccat ccgcgaagag
    39421 gaaggtggta agaagcagat tgcgctgacc gaacaaggcg cgcagtggct ggaagaaaac
    39481 cgcgaacagg tggagatgat tgaagaacgc atcaaagcgc gttgcgttgg cgctgcgctg
    39541 cgccagaacc cgcaaatgaa gcgggcgcta gataatttta aagcggtgct gaatttacgc
    39601 gtcaaccaga gtgatatcac tgatgcacaa ataaaaaaga tcattgcggt gatcgaccgc
    39661 gccgcttttg atattacgca actggattaa tcgccgcatc cgccagtggc gcggtgcaat
    39721 tgccggatgc gacgcttgac gcaccttatc cggcctacac ccgctacaca ccccgtaggc
    39781 ctgataagat gcgccagcat cgcatcaggc atcgtgtatc aaataccgga tccggcatac
    39841 cgattaatgc agcaccgtca ccgcgtcttc cagtcggctg gcgcggtgtt tcaccatcgc
    39901 cgacacctgc gcactctctt ccaccagctc ggcatttttc tgggtgatga ggttaagctc
    39961 atccactgca cgggtcaggc tggaaagacc atcggcctgt tccagcgttg aatggctaat
    40021 ctgggcaatc aactgggtga cgtttttcac ctgtgccaca atatcttcca tcgtacgtcc
    40081 ggcggcgtgt acctgctgcg aaccggattg caccttatca gcactggcat caatcagctt
    40141 gcgaatatcg ttggcagcat tggcgctgcg gctggctaaa tgacgcactt cccccgccac
    40201 caccgcaaaa cctttgccct gttcgccggc acgcgccgct tccaccgccg cattcagggc
    40261 cagaatattg gtctgaaacg caatatcgtt aatcagcgaa gtaatggtgc caatgcgctg
    40321 ggtactgtcg gcgatatcgt ccattgtctt gatcaccgtg gtcatcgcct ccccgccctg
    40381 cactgccgca ttactggcgg tgattgacag tttatcggca gccgacgccg tggcagagtt
    40441 ctgtttcacc gacgccgcca tttggttcat ggtcgccacc gtttgctgaa cgttatcaac
    40501 tgtctgctgg gtatgttcgt tcagttcatc ggtgcctttc gccagcgtct cactgccgtt
    40561 tctgacactg gacacctggc ttgagacatc gttaattaac caacggcaca tcaggccaag
    40621 ctgccctacc gcgcgtaatg tcagccccag ctcatcgctg cgattcagat gctcaacact
    40681 attacgctct ccggtcgcca ccttcagtgc ctgacgggcg acattttcta tcgggcggac
    40741 aatctgccat tcaaaacagg cgcttgccaa caataccact aacgcacaga ggatatacgt
    40801 caccaccggg gcagcaacaa accaaagcat ggccgccagc aagataaaca tcagggtcat
    40861 cactccacgc gcccgccagc gaagcggtaa tgaaggcagt ttacccagcc agcctttacg
    40921 caccaccagg cctttatgaa tacgcttacc ggtacgtccg gcgttcagcg ctttgtacag
    40981 cggctccacc gccgcgatct cttcatccgt cgcccgggta cgaatcgaca tatagccact
    41041 gatttttccc tcgcgcacca tcggtaccgc attggcccgc acccaataat ggccaccatt
    41101 tttgcggcga tttttcacga tgccgctcca gggctcccct tttttcaggg tgaaccacat
    41161 atccgcaaac gccgcttttg gcatatccgg gtgacgcacc atgttgtgcg gctgcccttg
    41221 taactcttgc aaggtaaagc cgctcacctg cacaaaagtg tcattagcat gagtgatata
    41281 gctttgcaga tcggtagtgg acatcagagt ggtatcgtcc gccagcgggg tattttgctg
    41341 ggtgacatac ggatgagaag acatgttcgc gtcctgtgca ggttatatgg ttgttaactt
    41401 cttgtcagag tttatgtcgg ccccgctgcg gttatcttta accgattaat ttgatttagc
    41461 tcgcaatttg cgattaaagc acaaatctaa ttcgttgatt taaaatactt tcactctgtt
    41521 actatacgaa aacgttaatt atcttgccca aaaatcaggc agttaatgcc ctgaaagcgt
    41581 gcatttgcgc agcaattatc aaatccatac ccgacaaaaa ccgtgcaaaa taacaacaga
    41641 tgttaacaaa tagcattaaa tattgcgcaa atgataacca catttgtgtt tatcccgatt
    41701 ttcgcgatcg cagccggagt ggcgcaatcc ctgcaatact taaatcggta tcatgtgata
    41761 cgcgagcctc cggagcatat tttgaacagg ttaccttcga gcgcatcggc tttagcgtgc
    41821 agcgcccacg ccctgaatct cattgaaaag cgaacgctgg atcatgagga gatgaaagca
    41881 cttaaccgag aggtgattga atacttcaaa gagcatgtca atccggggtt tttagagtat
    41941 cgcaaatctg ttaccgccgg cggggattac ggagccgtag agtggcaagc gggaggttta
    42001 aatacgcttg tcgacaccca gggacaggag tttatcgact gcctgggagg ttttggaatt
    42061 ttcaacgtgg ggcaccgtaa tccagttgtg gtttccgccg tacagaatca acttgcgaaa
    42121 caaccactgc acagccagga gctactcgat ccgttacggg ctatgctggc gaaaaccctt
    42181 gctgcgctaa cgccaggtaa actgaaatac agcttcttct gtaatagcgg caccgagtcc
    42241 gttgaagcgg cgctgaagct ggcgaaggct tatcagtcgc cgcgcggcaa atttactttt
    42301 attgccacca gcggcgcgtt ccacggtaaa tcacttggcg cgctgtcggc cacggcgaaa
    42361 tcgaccttcc gcaaaccgtt tatgccactg ctgccagggt tccgtcatgt gccgtttggc
    42421 aatatcgaag ccatgcgcac ggctcttaat gagtgcaaaa aaaccggtga tgatgtggcc
    42481 gcggtgatcc tcgaaccgat tcagggcgaa ggtggcgtaa ttctgccgcc gccgggctat
    42541 ctcaccgccg tgcgtaagct gtgcgatgag ttcggcgcac tgatgatcct cgatgaagta
    42601 caaacgggca tggggcgcac gggcaagatg ttcgcctgcg aacatgagaa cgtacagccg
    42661 gacatcctgt gcctggcaaa agccctcggc ggcggtgtga tgccgattgg cgcaaccatc
    42721 gccactgaag aggtgttctc agttctgttc gacaacccgt tcctgcatac caccaccttt
    42781 ggcggcaacc cgctggcctg tgcggcggcg ctggcgacca tcaatgtgtt gctggagcag
    42841 aacttaccgg ctcaggctga gcaaaaaggc gatatgttgc tggacggttt ccgtcaactg
    42901 gcgcgggaat atcccgatct ggtacaggaa gcgcgtggta aagggatgtt aatggcgatt
    42961 gagtttgttg ataacgaaat cggctataac tttgccagcg agatgttccg ccagcgcgta
    43021 ctggtggccg gaacgctcaa taacgccaaa acgatccgca ttgaaccgcc actgacactg
    43081 accattgaac agtgtgaact cgtgatcaaa gcggcgcgta aggcgctggc ggccatgcga
    43141 gtaagtgtcg aagaagcgta gtatcaaatc ggatggcaat acgacgtcgc catccgatct
    43201 ttttatacga tacgtacgcc cgcaggcatc atccgctccg gcgttaacag cacgctttca
    43261 ctgccgtcat cggtttccgc acacaacagc atgcattccg acgtttcacc acgcattttc
    43321 gctttttgca gattgcataa gaccaccacc gtttttccca tcaactcttc ttcgctgtag
    43381 tacggcacca ggctggtcac ggtttgcagt gttttttccc ccacatcaac ctgtacgatg
    43441 tacagcttgt cggcgttttc atggcgtttc acttccacaa tctttccgac gcgcatttcc
    43501 agacgtgcaa aatcagcgta agccacggtt tccattgctt cctcccttcg agtaaaattt
    43561 tactaaacta tagcaaaagt ttttctcaat cctgtaggct aaaaatggag aatgcaggcg
    43621 tgatcacatt cgtgagccgc tgtgttaccg ttacagcgtc aaagaaacgc gctttattta
    43681 ctgaaaacag gtgacccgat aagcacttcc tctacaatgg gggcgcacat cagggaaagt
    43741 aaaaaaggta aacatggcaa cactaaaaga catcgcaatc gaagctggcg tatccctggc
    43801 gacagtatcc agggtcttaa atgacgatcc gacattgaat gtgaaagaag agacgaaaca
    43861 tcgcattctc gagatcgccg aaaagctgga gtacaagacc agtagcgccc gtaaactaca
    43921 gacaggcgca gtcaaccaac accatattct ggctatctac agctaccagc aggagctgga
    43981 gatcaacgat ccttactatc tggcgatccg ccacggcatt gaaactcagt gcgaaaagct
    44041 ggcgatcgaa ctgaccaact gttatgaaca cagcggatta ccagacatta aaaacgtcac
    44101 cggtatttta attgtcggca aacccacgcc cgccctgcgc gccgctgcca gcgcgttgac
    44161 cgacaatatc tgttttatcg actttcacga acccggcagc ggttacgatg cggtggatat
    44221 cgatctggca cgcatcagta aagaaatcat cgacttctat atcaaccagg gcgttaatcg
    44281 tattggtttt attggcggtg aagatgagcc tggcaaggcg gatattcgtg aggtcgcctt
    44341 tgcggaatat ggccgactga aacaagtggt acgcgaagag gatatctggc gcggcggttt
    44401 ttccagttcg tcgggttatg aactggcaaa acaaatgctg gcgcgggaag actatccgaa
    44461 ggcactgttt gttgcttccg attccattgc tatcggcgta ctgcgggcga ttcatgaacg
    44521 tggtctgaac atcccacagg atatttcgct tatcagcgtt aacgatatcc ccaccgcgcg
    44581 atttaccttt ccgccgctct ccaccgtgcg catccattcc gaaatgatgg gaagtcaggg
    44641 cgtaaacctg gtgtatgaaa aagcccgcga tggtcgcgcg ctgccgctgt tagtcttcgt
    44701 tcccagcaaa ttaaaactgc gcggcacgac ccgttaaatc cccttacaca ctgtccggca
    44761 atcgtttttg ccgggcagtg ctgccgttta ttttcgtgat ccagttaaag taaatgcatt
    44821 tacccgctac tttttagtaa aaattttact aaactcccca gcaattacac aaactaccat
    44881 caccatgaat ggttccgatt tctctctacc gggaggccct atgaatcgct gggaaaacat
    44941 tcagctcacc cacgaaaacc gacttgcgcc gcgtgcgtac tttttttcat atgattctgt
    45001 tgcgcaagcg cgtacctttg cccgcgaaac gagcagcctg tttctgccct taagcggtca
    45061 gtggaatttc cacttttttg accatccgct gcaagtgcca gaagccttca cctctgagtt
    45121 aatggctgac tgggggcata ttaccgtccc cgccatgtgg caaatggaag gtcacggcaa
    45181 actgcaatat accgacgaag gttttccgtt ccccatcgat gtgccgtttg ttcccagcga
    45241 taacccaacc ggtgcctatc aacgtatttt caccctcagt gacggctggc agggtaaaca
    45301 gacgctgatt aaatttgacg gcgtcgaaac ctattttgaa gtctacgtta acggtcagta
    45361 tgtgggtttc agcaagggca gtcgcctgac cgcagagttt gacatcagcg cgatggttaa
    45421 aaccggcgac aacctgttgt gtgtgcgcgt gatgcagtgg gcggactcta cctacgtgga
    45481 agaccaggat atgtggtggt cagcggggat cttccgcgat gtttatctgg tcggaaaaca
    45541 cctaacgcat attaacgatt tcactgtgcg taccgacttt gacgaagcct attgcgatgc
    45601 cacgctttcc tgcgaagtgg tgctggaaaa tctcgccgcc tcccctgtcg tcacgacgct
    45661 ggaatatacc ctgtttgatg gcgaacgcgt ggtgcacagc agcgccattg atcatttggc
    45721 aattgaaaaa ctgaccagcg ccagctttgc ttttactgtc gaacagccgc agcaatggtc
    45781 agcagaatcc ccttatcttt accatctggt catgacgctg aaagacgcca acggcaacgt
    45841 tctggaagtg gtgccacaac gcgttggctt ccgtgatatc aaagtgcgcg acggtctgtt
    45901 ctggatcaat aaccgttatg tgatgctgca cggcgtcaac cgtcacgaca acgatcatcg
    45961 caaaggccgc gccgttggaa tggatcgcgt cgagaaagat ctccagttga tgaagcagca
    46021 caacatcaac tccgtgcgta ccgctcacta cccgaacgat ccgcgttttt acgaactgtg
    46081 tgatatctac ggcctgtttg tgatggcgga aaccgacgtc gaatcgcacg gctttgctaa
    46141 tgtcggcgat atcagccgta ttaccgacga tccgcagtgg gaaaaagtct acgtcgagcg
    46201 cattgttcgc catattcacg cgcagaaaaa ccatccgtcg atcatcatct ggtcgctggg
    46261 caatgaatcc ggctatggct gtaacatccg cgcgatgtac cacgcagcga aggcgctgga
    46321 tgacacgcga ctggtgcatt acgaagaaga tcgcgatgct gaagtggtcg atattatttc
    46381 caccatgtac acccgcgtgc cgctgatgaa tgagtttggt gaatacccgc atccgaagcc
    46441 gcgcatcatc tgtgaatatg ctcatgcgat ggggaacgga ccgggcgggc tgacggagta
    46501 ccagaacgtc ttctataagc acgattgtat tcagggacat tatgtttggg agtggtgcga
    46561 ccacgggatc caggcgcagg atgacaacgg caatgtctgg tataaattcg gcggcgacta
    46621 cggcgactat cccaacaact ataacttctg tcttgatggt ttgatctatt ccgatcagac
    46681 gccgggacca ggcctgaaag agtacaaaca ggttatcgcg ccggtaaaaa tccacgcgct
    46741 ggatctgact cgcggcgagc tgaaagtcga aaataaactg tggtttacca cgctggatga
    46801 ctacaccctg cacgcagagg tgcgcgccga aggtgaaacg ctcgcgacgc aacagattaa
    46861 actgcgcgac gttgcgccga acagcgaagc ccccttgcag atcacgctgc cgcagctgga
    46921 cgcccgcgaa gcgttcctca acattacggt gaccaaagat tcccgcaccc gctacagcga
    46981 agccgggcat tctatcgcca cttatcagtt cccgctgaag gaaaacaccg cgcagcctgt
    47041 acctttcgca ccgaataacg cccgtccgct gacgctggaa gacgatcgtt tgagctgtac
    47101 tgttcgcggc tacaactttg cgatcacctt ctcaaaaacg agtggtaaac cgacatcctg
    47161 gcaggtaaat ggcgaatcgc tgctgacccg cgagccaaag atcaacttct tcaagccaat
    47221 gatcgacaac cacaagcagg agtacgaagg gttgtggcag ccgaatcatt tgcagatcat
    47281 gcaggaacat ctgcgcgact ttgccgtaga acagagcgat ggtgaagtgt tgatcatcag
    47341 ccgcacggtt atagcaccgc cggtgtttga cttcgggatg cgctgtacct acatctggcg
    47401 catcgctgca gatggccagg ttaacgtggc gctttccggc gagcgttacg gcgactatcc
    47461 gcacatcatt ccgtgcatcg gtttcaccat ggggattaac ggcgaatacg atcaggtggc
    47521 gtattacggt cgtggaccgg gcgaaaacta cgccgacagc cagcaggcta acatcatcga
    47581 tatctggcgc agcaccgtcg atgccatatt cgagaactat cccttcccgc agaacaacgg
    47641 caaccgtcag catgtccgct ggacggcact gactaaccgc cacggcaacg gtctgctggt
    47701 ggttccgcag cgcccaatta acttcagcgc ctggcactat acccaggaaa acatccacgc
    47761 tgcccagcac tgtaacgagc tgcagcgcag tgatgacatc accctgaatc tcgaccacca
    47821 gctgcttggc ctcggctcca attcctgggg cagcgaggtg ctggactcct ggcgcgtctg
    47881 gttccgtgac ttcagctacg gctttacgtt gctgccggtt tctggcggag aagctaccgc
    47941 gcaaagcctg gcgtcgtatg agttcggcgc agggttcttt tccacaaatt tgcacagcga
    48001 gaataagcaa tgaggatcat cgataactta gaacagttcc gccagattta cgcctctggt
    48061 aagaagtggc aacgctgcgt tgaagcgatt gaaaatatcg acaacattca gcctggcgtc
    48121 gcccactcca tcggtgactc attgacttac cgcgtggaga cagactccgc gaccgatgcg
    48181 ctatttaccg ggcatcgacg ctattttgaa gtgcattact acctgcaagg gcagcaaaaa
    48241 attgaatatg cgccgaaaga gacattacag gtcgtggaat attatcgtga tgaaactgac
    48301 cgtgaatatt taaaaggctg cggagaaacc gttgaggtcg acgaagggca aatcgttatt
    48361 tgcgatatcc atgaagcata tcggtttatc tgcaataacg cggtcaaaaa agtagttctc
    48421 aaagtcacca tcgaagatgg ttatttccat aacaaataac aactacggcg gcaaaaggag
    48481 tttgccgcca ccgctaccct actcattttc ggagatgtgt tatgtctgat accaaacgta
    48541 atacaatcgg caaattcggc ttgctctcgc tgacttttgc cgccgtttac agctttaaca
    48601 acgttatcaa taataatatt gagcttggac tggcctcggc accgatgttt ttcctcgcga
    48661 cgatttttta ttttattccc ttctgtctga tcatcgcaga atttgtttcg ttaaataaaa
    48721 actcagaagc cggtgtctac gcgtgggtaa aaagttcgct gggcggacgt tgggcattta
    48781 ttactgccta tacctactgg ttcgtaaacc tgttcttttt cacctcgctg ttgccgcgcg
    48841 ttattgctta tgcttcgtat gccttcctcg gctatgaata tattatgacg ccggttgcca
    48901 ccaccattat cagtatggtg ctgttcgcct tctccacctg ggtttccacc aacggggcga
    48961 aaatgttggg gccaattacc tccgtcactt caacgctgat gctgctgtta acgctctcct
    49021 acattttact ggcaggtacg gcgctggttg gcggcgtaca gcctgctgac cccatcaccg
    49081 ttgacgcgat gatcccgaac ttcaactggg cgttcctcgg cgttaccacc tggatcttta
    49141 tggccgcagg tggcgcggag tccgtcgctg tgtacgttaa cgacgtcaaa ggcggttcga
    49201 aatcgttcgt taaagtgatc atcctcgccg ggatttttat cggtgtacta tattccgtct
    49261 cctcggtgct gattaacgtc ttcgtcagca gcaaagagtt gaaatttact ggcggatcgg
    49321 tgcaggtatt ccacggcatg gcggcgtatt ttggtctgcc ggaagcgttg atgaatcgct
    49381 ttgtcggtct ggtgtccttt accgcaatgt tcggttccct gctgatgtgg accgcaacgc
    49441 cggtgaaaat tttcttctcc gaaatcccgg aaggcatctt tggtaagaaa accgtcgaac
    49501 ttaacgaaaa cggcgttccg gcgcgcgcag cgtggatcca gttcctgatc gtcatcccgc
    49561 tgatgattat cccgatgctc ggttccaata ctgtgcagga tctgatgaat actattatta
    49621 atatgaccgc cgcagcgtcc atgcttccgc cgttattcat catgctggct tacctgaatt
    49681 tacgcgccaa attagatcac ctgccacgcg atttccgtat gggctcccgc cgcaccggta
    49741 ttatcgttgt ttcaatgctg attgcgatat ttgccgtagg gtttgtcgct tcgacattcc
    49801 cgactggcgc gaatattctg accatcattt tttataacgt cggcggtatt gttatattcc
    49861 ttggctttgc gtggtggaaa tacagtaaat atataaaggg attaacggct gaagagcgcc
    49921 atattgaagc gacgccagcc agcaatgttg attaaacaga ctaataaaaa atgagcaggc
    49981 attttccctc ttcctggatg aggagagg
//
LOCUS       DAZFDC010000017        48259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFDC010000017 DAZFDC010000000
VERSION     DAZFDC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.017"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(43..1455)
                     /gene="pykF"
                     /locus_tag="ACHJ48_001151"
     CDS             complement(43..1455)
                     /gene="pykF"
                     /locus_tag="ACHJ48_001151"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO4706755.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1463..1657)
                     /locus_tag="ACHJ48_001152"
     CDS             complement(1463..1657)
                     /locus_tag="ACHJ48_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706756.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2012..2221
                     /gene="fumD"
                     /locus_tag="ACHJ48_001153"
     CDS             2012..2221
                     /gene="fumD"
                     /locus_tag="ACHJ48_001153"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO4706757.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            2677..3303
                     /gene="ydhY"
                     /locus_tag="ACHJ48_001154"
     CDS             2677..3303
                     /gene="ydhY"
                     /locus_tag="ACHJ48_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO4706758.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            3324..5426
                     /gene="ydhV"
                     /locus_tag="ACHJ48_001155"
     CDS             3324..5426
                     /gene="ydhV"
                     /locus_tag="ACHJ48_001155"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO4706759.1"
                     /translation="MTNGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAECWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            5439..6077
                     /gene="ydhW"
                     /locus_tag="ACHJ48_001156"
     CDS             5439..6077
                     /gene="ydhW"
                     /locus_tag="ACHJ48_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO4706760.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            6141..6809
                     /gene="ydhX"
                     /locus_tag="ACHJ48_001157"
     CDS             6141..6809
                     /gene="ydhX"
                     /locus_tag="ACHJ48_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4706761.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHAPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            6806..7591
                     /gene="phsC"
                     /locus_tag="ACHJ48_001158"
     CDS             6806..7591
                     /gene="phsC"
                     /locus_tag="ACHJ48_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO4706762.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINGVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            7595..8407
                     /gene="ydhT"
                     /locus_tag="ACHJ48_001159"
     CDS             7595..8407
                     /gene="ydhT"
                     /locus_tag="ACHJ48_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO4706763.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGKRCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(8419..10023)
                     /gene="ydhS"
                     /locus_tag="ACHJ48_001160"
     CDS             complement(8419..10023)
                     /gene="ydhS"
                     /locus_tag="ACHJ48_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO4706764.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDQFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(10149..10454)
                     /gene="ydhR"
                     /locus_tag="ACHJ48_001161"
     CDS             complement(10149..10454)
                     /gene="ydhR"
                     /locus_tag="ACHJ48_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO4706765.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTAQLKKLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(10562..10638)
                     /locus_tag="ACHJ48_001162"
     tRNA            complement(10562..10638)
                     /locus_tag="ACHJ48_001162"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10602..10604),aa:Val,seq:gac)
     gene            complement(10643..10719)
                     /locus_tag="ACHJ48_001163"
     tRNA            complement(10643..10719)
                     /locus_tag="ACHJ48_001163"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10683..10685),aa:Val,seq:gac)
     gene            11027..12283
                     /gene="ydhQ"
                     /locus_tag="ACHJ48_001164"
     CDS             11027..12283
                     /gene="ydhQ"
                     /locus_tag="ACHJ48_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO4706766.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLADGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVQITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(12324..13697)
                     /gene="mdtK"
                     /locus_tag="ACHJ48_001165"
     CDS             complement(12324..13697)
                     /gene="mdtK"
                     /locus_tag="ACHJ48_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO4706767.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRYLQRLPSAI
                     ILQRASR"
     gene            13912..14553
                     /gene="ribE"
                     /locus_tag="ACHJ48_001166"
     CDS             13912..14553
                     /gene="ribE"
                     /locus_tag="ACHJ48_001166"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO4706768.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQSGTEA"
     gene            complement(14593..15741)
                     /gene="cfa"
                     /locus_tag="ACHJ48_001167"
     CDS             complement(14593..15741)
                     /gene="cfa"
                     /locus_tag="ACHJ48_001167"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO4706769.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFTVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFVAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(16032..17243)
                     /gene="punC"
                     /locus_tag="ACHJ48_001168"
     CDS             complement(16032..17243)
                     /gene="punC"
                     /locus_tag="ACHJ48_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO4706770.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAAMLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            17356..18288
                     /gene="punR"
                     /locus_tag="ACHJ48_001169"
     CDS             17356..18288
                     /gene="punR"
                     /locus_tag="ACHJ48_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO4706771.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(18285..19310)
                     /gene="purR"
                     /locus_tag="ACHJ48_001170"
     CDS             complement(18285..19310)
                     /gene="purR"
                     /locus_tag="ACHJ48_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO4706772.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            19609..19698
                     /gene="cydH"
                     /locus_tag="ACHJ48_001171"
                     /gene_synonym="ynhF"
     CDS             19609..19698
                     /gene="cydH"
                     /locus_tag="ACHJ48_001171"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO4706773.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            19864..21033
                     /gene="ydhP"
                     /locus_tag="ACHJ48_001172"
     CDS             19864..21033
                     /gene="ydhP"
                     /locus_tag="ACHJ48_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4706774.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNKFGAAISMAVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(21179..21760)
                     /gene="sodB"
                     /locus_tag="ACHJ48_001173"
     CDS             complement(21179..21760)
                     /gene="sodB"
                     /locus_tag="ACHJ48_001173"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO4706775.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21888..22703)
                     /gene="mepH"
                     /locus_tag="ACHJ48_001174"
     CDS             complement(21888..22703)
                     /gene="mepH"
                     /locus_tag="ACHJ48_001174"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO4706776.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            23038..23385
                     /gene="grxD"
                     /locus_tag="ACHJ48_001175"
     CDS             23038..23385
                     /gene="grxD"
                     /locus_tag="ACHJ48_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO4706777.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(23436..28052)
                     /gene="lhr"
                     /locus_tag="ACHJ48_001176"
     CDS             complement(23436..28052)
                     /gene="lhr"
                     /locus_tag="ACHJ48_001176"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO4706778.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAADAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVELVIQVATPLSVASGLQRIGRAGHQIGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSDGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPAILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVWQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(28145..28792)
                     /gene="rnt"
                     /locus_tag="ACHJ48_001177"
     CDS             complement(28145..28792)
                     /gene="rnt"
                     /locus_tag="ACHJ48_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO4706779.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(28895..29302)
                     /gene="gloA"
                     /locus_tag="ACHJ48_001178"
     CDS             complement(28895..29302)
                     /gene="gloA"
                     /locus_tag="ACHJ48_001178"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO4706780.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(29383..30480)
                     /gene="nemA"
                     /locus_tag="ACHJ48_001179"
     CDS             complement(29383..30480)
                     /gene="nemA"
                     /locus_tag="ACHJ48_001179"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO4706781.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPSI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(30517..31116)
                     /gene="nemR"
                     /locus_tag="ACHJ48_001180"
     CDS             complement(30517..31116)
                     /gene="nemR"
                     /locus_tag="ACHJ48_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO4706782.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            31219..31458
                     /gene="ydhL"
                     /locus_tag="ACHJ48_001181"
     CDS             31219..31458
                     /gene="ydhL"
                     /locus_tag="ACHJ48_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO4706783.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            31507..32403
                     /gene="ydhF"
                     /locus_tag="ACHJ48_001182"
                     /pseudo
     CDS             31507..32403
                     /gene="ydhF"
                     /locus_tag="ACHJ48_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
     gene            32484..33005
                     /gene="sodC"
                     /locus_tag="ACHJ48_001183"
     CDS             32484..33005
                     /gene="sodC"
                     /locus_tag="ACHJ48_001183"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4706784.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(33006..35018)
                     /gene="ydhK"
                     /locus_tag="ACHJ48_001184"
     CDS             complement(33006..35018)
                     /gene="ydhK"
                     /locus_tag="ACHJ48_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4706785.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSDVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYNFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGIVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(35018..35875)
                     /gene="ydhJ"
                     /locus_tag="ACHJ48_001185"
     CDS             complement(35018..35875)
                     /gene="ydhJ"
                     /locus_tag="ACHJ48_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO4706786.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(35878..36114)
                     /gene="ydhI"
                     /locus_tag="ACHJ48_001186"
     CDS             complement(35878..36114)
                     /gene="ydhI"
                     /locus_tag="ACHJ48_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO4706787.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            36315..36749
                     /gene="slyA"
                     /locus_tag="ACHJ48_001187"
     CDS             36315..36749
                     /gene="slyA"
                     /locus_tag="ACHJ48_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO4706788.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(36796..37263)
                     /gene="slyB"
                     /locus_tag="ACHJ48_001188"
     CDS             complement(36796..37263)
                     /gene="slyB"
                     /locus_tag="ACHJ48_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO4706789.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            37536..38645
                     /gene="anmK"
                     /locus_tag="ACHJ48_001189"
     CDS             37536..38645
                     /gene="anmK"
                     /locus_tag="ACHJ48_001189"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO4706790.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVKALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            38743..39072
                     /gene="mliC"
                     /locus_tag="ACHJ48_001190"
     CDS             38743..39072
                     /gene="mliC"
                     /locus_tag="ACHJ48_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO4706791.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            39131..39787
                     /gene="pdxH"
                     /locus_tag="ACHJ48_001191"
     CDS             39131..39787
                     /gene="pdxH"
                     /locus_tag="ACHJ48_001191"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO4706792.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGSWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            39916..41190
                     /gene="tyrS"
                     /locus_tag="ACHJ48_001192"
     CDS             39916..41190
                     /gene="tyrS"
                     /locus_tag="ACHJ48_001192"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO4706793.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            41252..42112
                     /gene="pdxY"
                     /locus_tag="ACHJ48_001193"
     CDS             41252..42112
                     /gene="pdxY"
                     /locus_tag="ACHJ48_001193"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFO4706794.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(42156..42761)
                     /gene="gstA"
                     /locus_tag="ACHJ48_001194"
     CDS             complement(42156..42761)
                     /gene="gstA"
                     /locus_tag="ACHJ48_001194"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO4706795.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALEDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(42867..44369)
                     /gene="dtpA"
                     /locus_tag="ACHJ48_001195"
     CDS             complement(42867..44369)
                     /gene="dtpA"
                     /locus_tag="ACHJ48_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO4706796.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(44980..45615)
                     /gene="nth"
                     /locus_tag="ACHJ48_001196"
     CDS             complement(44980..45615)
                     /gene="nth"
                     /locus_tag="ACHJ48_001196"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO4706797.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNS
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(45615..46310)
                     /gene="rsxE"
                     /locus_tag="ACHJ48_001197"
     CDS             complement(45615..46310)
                     /gene="rsxE"
                     /locus_tag="ACHJ48_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO4706798.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(46314..46934)
                     /gene="rsxG"
                     /locus_tag="ACHJ48_001198"
     CDS             complement(46314..46934)
                     /gene="rsxG"
                     /locus_tag="ACHJ48_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO4706799.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGNFDQFTGATITPRAVVNAVKRAGLYAQTLPEQLSQLPACGE"
     gene            complement(46938..47996)
                     /gene="rsxD"
                     /locus_tag="ACHJ48_001199"
     CDS             complement(46938..47996)
                     /gene="rsxD"
                     /locus_tag="ACHJ48_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO4706800.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(47997..>48259)
                     /locus_tag="ACHJ48_001200"
     CDS             complement(47997..>48259)
                     /locus_tag="ACHJ48_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO4706801.1"
                     /translation="VEAAITRAKARKLEQQQANAEPEEQVDPRKAAVAAAIARAKARK
                     LEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAVQQKVVNED"
BASE COUNT        12179 a        11850 c        12504 g        11726 t
ORIGIN      
        1 atcagggcgc ttcgatatac aaattaattc acaaaagcaa tattacagga cgtgaacaga
       61 tgcggtgtta gtagtgccgc tcggtaccag tgcaccagaa accataacta caacgtcacc
      121 tttgtgtgcc agaccgctct gcagagccag ttctttaccc agacggtaga aatcatcagt
      181 agaagtgatc tctttaacca gctgcggcac aacgcctttg ctcagtacca actgatgagc
      241 cgttttttcg ttggtggtca gtgccaggat ggtggcatcc gggaagtatt tacgtactgc
      301 gcgagcagat ttaccgccct gggtagcaac cacgatcagc ggagcatcca gtttttcagc
      361 agtttcaacg gcaccacggc ataccgcttc ggtaatgcgc agtttacggt tgtcattgtt
      421 gaactcgaga cggctgttca tcacgcggtc ggtacgttcg cagatggtcg ccatgataga
      481 aaccgcttcc agcgggtatt taccttttgc ggattcacca gacagcatca ctgcgtcagt
      541 accgtcgagg atggcgtttg caacgtcacc ggcttctgcg cgagtcgggc gtgggttttt
      601 gatcatggaa tccagcatct gggtcgcagt gataacgact ttacgtgcac ggatacattt
      661 ttcgatcatc atcttctggg cgaagataac ttcttctacc gggatttcta cacccaggtc
      721 gccacgcgca accatgatgc cgtcagaggc ttcgaggatt tcgtcgaagt tgttgaggcc
      781 ttcctggttt tcgattttgg agatgatgtg gatgttttcg ccgccgtgcg ctttcaggtg
      841 ctcacggatt tcgataacgt cagaacgctt acgaataaag gaagcagcga caaagtctac
      901 gccttgttcg caaccaaaga tcaggtcctg tttgtctttt tcagccagtg ctggcagggc
      961 aatggaaacg ccaggcaggt tcacaccttt gttttcaccc aggtcgccgt tgttcagcac
     1021 tttacagata actttgttac cttcaatggc agtaacttcc ataccgatca gaccatcgtc
     1081 aaccagtacg gtgttgccaa cagacaggtc agttgtgaaa ccttcatacg ttaccgcaac
     1141 catttcgctg ttgccgataa cagatttatc agtagtgaaa gtaaaggtct gaccagcttt
     1201 cagagaaacg tcgttaccgc cttccagttt catggtgcgg atttccggac ctttggtatc
     1261 aagcaggata gcggcggttt taccagtttt gctcatcacg ttgcgcagat tctgaatgcg
     1321 ctgaccgtgt tctgcatagt caccatgaga gaagttcaga cgcataacgt tcatgccagc
     1381 gtccagcatt ttagctaaca tctcttcaga ttcggttttc ggtccgatgg tgcaaacaat
     1441 tttggtcttt ttcatgacag tcttagtctt taagttgaga aggatgggag aaacttgctt
     1501 tctgggcgca ctgccggaaa gcgaatccgg tattacgaaa gtggtggtgc ctcgctctaa
     1561 ggataggtga catcgaaaaa gcgtgcagag gaatgtgtgc ttgtgtctca gcccaacgtg
     1621 agtgcctgtt tttgagtcgt tgagttctac agtccaaagt gctgaaacca ttcaagagtc
     1681 aattggcgcg cattatacgc tgaaattaat ccaaaaggaa aacaaaaaca gcgtttcaaa
     1741 aaatatacaa cttgttagac aataaattgt tatcaaagat ttaacgttcc agggaaaagg
     1801 ttacgcaaaa acttcgctgt ttgcgcaacg tctggtgccg gggttgtcac tgtgaagata
     1861 agtgaaagtt catccggtgt ataaatacat catcgctcaa ttgctacagt taaaataatt
     1921 tatcgcgtta tttgtctgat tttgtttgat ttttgtttaa ttggttagat tgatatatgc
     1981 tgatagttgg catgataaat aggtataaag aatgggaaat cggacaaaag aggatgaact
     2041 gtaccgagaa atgtgcagag ttgtcggtaa agttgtgctg gaaatgcgcg atctggggca
     2101 ggaaccaaag catattgtta tcgcaggcgt attacgtaca gcattagcca ataaacgcat
     2161 tcagcgcagt gagctggaaa agcaggcgat ggaaaccgtc attaacgcac tggtaaaatg
     2221 accattcagg atgaggcgat aagcttcatc ctgatattct tgcttcagtt gcggtccagt
     2281 taatcattct aaataacaac tgctgaaatt atacgaattt cgatatgtgt tttaatttgt
     2341 aacgataata ttatttttat tttctgcaat gaccgtcaca aataattgtc gcttaagtta
     2401 aattcatttc aaatgataat aaatatcagt tcacttataa tttcttgctt tgtaatttta
     2461 attgataatg atcaatgtta agagcccaga ataaccacat tattttttct cctaaccata
     2521 aaggattagt ttatcggcgt taaatatttt ctcatacact atcgggttag ggaaaatatc
     2581 tggtgttggg gacagagtca gcagcgtatt agttgaatgc ttaatatcag gctgctgaat
     2641 tgtcagcact gtgtttgcga agaggatgcg aaataaatga acccggttga tcgtccacta
     2701 ttagatattg gcttaacgcg attagaattc ttgcgtatat caggaaaagg ccttgctggt
     2761 ctaacaattg cccctgcgtt actgtcactt ttgggttgca aacaggaaga tattgatagc
     2821 ggcacagtag ggttgataaa tacacccaaa ggggtgctgg tgacccagcg agcgcgatgc
     2881 acgggttgcc accgctgtga aatctcttgt accaacttca acgatggctc agtagggaca
     2941 ttcttctccc gtatcaaaat ccatcgcaat tatttctttg gcgacaacgg ggttggctct
     3001 ggcggcggcc tgtatggcga tctcaactat accgcggaca cctgccgtca atgcaaagag
     3061 ccgcaatgca tgaacgtctg cccgattggt gcgattacct ggcagcagaa agaaggctgt
     3121 attaccgtcg atcataaacg ttgtattggc tgtagcgcct gtactacggc atgcccgtgg
     3181 atgatggcca ccgtaaatac cgaaagtaaa aaatcctcga aatgtgtgtt atgcggtgaa
     3241 tgcgcaaacg cctgcccaac aggggcgtta aaaattatcg agtggaaaga tattactgtg
     3301 tgaatcttgc aaaggaaaaa atcatgacta acggttggac aggtaatata ttaagagtca
     3361 atctcacgac aggaaatatt accctcgaag attccagtaa gtttaaaagt tttgtcggtg
     3421 gcatgggctt cggctacaaa attatgtatg acgaagtacc gccaggcacg aaacctttcg
     3481 atgaagcgaa taaattagtc tttgctaccg gcccattaac tggatctggt gccccctgta
     3541 gttctcgcgt aaatatcacc tcactttcta cttttaccaa aggaaattta gtcgtcgatg
     3601 cccatatggg tggctttttt gcagcgcaaa tgaaattcgc tggatacgac gtcattatta
     3661 tcgaagggaa ggcgaaatca ccggtatggc tgaagattaa agatgacaaa gttagcctgg
     3721 aaaaagcaga tttcttatgg ggaaaaggga cgcgcgcaac gacggaagaa atttgtcgct
     3781 tgaccagtcc ggaaacctgt gtggcggcta ttggtcaggc tggggaaaac cttgttcctc
     3841 tctctggcat gttgaatagc cgtaaccaca gcggcggtgc gggaactggc gcaataatgg
     3901 gttcgaaaaa cctgaaagcg attgcggttg aagggacgaa aggggtcaac attgccgatc
     3961 gtcaggagat gaagcgtctc aatgattaca tgatgactga acttatcggt gcgaataaca
     4021 accatgtcgt gccaagtacg ccgcaatcgt gggcagagta ttcagatccc aagtcacgct
     4081 ggacagcacg taaagggctg ttttggggcg cggctgaagg cggtccaatt gaaacgggtg
     4141 aaattccgcc aggcaatcag aatacggtcg gctttcgtac ctataaatcc gtttttgact
     4201 taggtccggc agcagagaaa tacacagtaa aaatgagcgg ctgccactct tgcccgatcc
     4261 gttgtatgac ccaaatgaat attcctcggg tgaaagagtt tggcgtgccc agcacaggtg
     4321 gtaacacttg tgtagcaaac tttgtccata ccaccatctt cccgaacggg ccgaaagatt
     4381 ttgaagataa agacgatggt cgtgtgattg gtaacctggt tggtctgaat ttgttcgatg
     4441 actacggcct atggtgtaac tacgggcagt tgcatcgcga ctttacttat tgttacagca
     4501 aaggtgtgtt caagcgtgtt ctgcctgctg aagagtatgc agaaattcgc tgggatcaac
     4561 tggaagcggg agacgttaac ttcattaaag atttttacta ccgtctggcg catcgtgtgg
     4621 gtgagctgag tcacctggct gatggttcat atgccatcgc agaatgctgg aatttgggtg
     4681 aagagtactg gggctacgcg aaaaataaac tctggtcgcc gtttggctat ccggttcacc
     4741 atgccaatga agcgtcagcg caggtcggtt ccattgttaa ctgtatgttc aaccgtgact
     4801 gcatgacgca tacccatatc aactttattg gttccggctt gccattgaaa ctgcaacgtg
     4861 aagtggcgaa agaacttttt ggttctgaag atgcttacga tgaaaccaaa aactacacgc
     4921 caatcaatga cgcaaaaatt aaatatgcta agtggtcgct gttgcgggtc tgtttgcata
     4981 acgccgtcac cctgtgcaac tgggtctggc caatgaccgt ttcgccgctg aaaagccgta
     5041 attaccgggg cgatctggcg cttgaagcca aattcttcaa agcgatcact ggcgaagaga
     5101 tgactcagga aaaattagat ttagctgcag agcgtatctt tacattgcat cgtgcctaca
     5161 cggtgaaact gatgcaaacc aaagatatgc gtaatgaaca cgatcttatc tgttcctggg
     5221 tattcgacaa ggatccgcag atcccggtct ttactgaagg tactgacaaa atggatcgtg
     5281 atgatatgca tgcctcgctc acgatgttct acaaagaaat gggctgggac ccacagcttg
     5341 gttgtccgac ccgcgaaaca ttgcagcgtc tggggctgga agatattgcc gccgatctgg
     5401 cagcacacaa tctactgcct gcgtaaggaa tgggtaagat gaaccatcag gatgaattgc
     5461 cgttagcgaa agtcagcgaa gttgacgagg cgaagcggca atggttgcag gggatgcgtc
     5521 accccgttga tacggtaacc gaaccagaac cggctgaaat cctggcagag ttcattcgcc
     5581 agcattctgt ggctgggcag ctggtggcgc gcgccgtgtt tctctcgcca ccctattcag
     5641 ttgcagaaga ggaactgtca gttttgctgg aaaacatcaa gcagaacgga gactacgcgg
     5701 atatcgcttg tatgacggga agtcaggatg attactatta ctcaacgcag gccatgagtg
     5761 aaaactacgc ggcgatgtct ttgcaagttg tggagcagga catttgtcgc gccattgcac
     5821 acgccgttcg ttttgagtgc cagacgtatc ctcgtcctta caaagtcgcc atgctgatgc
     5881 aagcgccata ttacttccag gaagcgcaga ttgaagccgc gatcgccgca atggatgttg
     5941 cacctgaata tgccgatata cggcaggtag agtcatcgac ggcggtgttg tacttgttta
     6001 gcgagcgatt tatgacctat ggcaaggctt atggcttatg cgaatggttc gaggtagagc
     6061 agtttcagaa cccctgaaat ttcagggaga tgtcatggat agggtggaca gttgcagcca
     6121 ctgcattagg ggataatcag atgtccttca ctcgacgcaa atttgttctg gggatgggaa
     6181 cagtaatctt ttttactggt tctgcgtctt ctctgttagc gaacacgagg caagaaaagg
     6241 aagttcggta tgccatgatt catgacgagt cacggtgcaa tggctgtaat atttgcgccc
     6301 gcgcatgccg taaaactaac cacgcccccg ctcagggaag ccgtttatcg atagcacaca
     6361 ttccagtcac cgataacgac aacgaaacgc agtatcactt ttttcgccag tcatgtcagc
     6421 actgcgaaga tgctccgtgc atcgacgtat gcccgacagg ggcgtcgtgg cgtgatgagc
     6481 aggggatcgt gcgggtagaa aaatcgcagt gcatcggctg cagttattgc attggcgctt
     6541 gtccgtacca ggttcgctac cttaaccctg tcactaaagt ggcagataaa tgtgatttct
     6601 gcgctgagtc ccgactggca aaaggctttc cgcccatttg cgtcagcgct tgcccggaac
     6661 acgcattgat ctttggtcgc gaagatagcc ctgaaattca ggcgtggttg caggataata
     6721 aatactatca atatcagcta cctggtgccg ggaaaccgca tctgtatcgc cggttcggtc
     6781 aacatttgat taaaaaggaa aatgtatgaa cccgtcgcaa catgctgaac agtttcagag
     6841 ccagttagcg aactatgtgc cacagttcac tcccgaattt tggccggtgt ggttgatcat
     6901 tgccggagta ttgctggttg ggatgtggtt ggtgctgggg ctgcacgcct tgcttcgtgc
     6961 tcgtggcgtg aagaaatcag ccaccgatca tggtgagagg atttatcttt acagcaaagc
     7021 ggtcagatta tggcactggt cgaatgcgtt actctttgta ttgttgctgg caagtgggct
     7081 gataaatcac tttgcgctgg tgggcgcaac tgcggttaaa agtctggttg cggtgcatga
     7141 agtttgcgga tttttgttac tggcatgctg gctcggcttt gttctgatca atggcgttgg
     7201 gggtaatggc caccactatc gcattcgtcg tcagggttgg ctggagcggg cggcaaaaca
     7261 aacgcgattt tatttgtttg gcattatgca gggggaagaa catcctttcc cggcaacaac
     7321 ccagtctaaa tttaatccct tacagcaggt tgcctatgtt ggtgtcatgt atggattgct
     7381 gccgttgtta ctattgacgg ggctgctgtg tctctatccg caagccgtgg gagatgtgtt
     7441 tcctggcgta agatactggt tattgcaggc gcattttgct ctggcattta taagcctctt
     7501 ttttatcttc ggtcatcttt atctttgcac cacggggcgt acgccacacg aaacctttaa
     7561 aagcatggtc gatggctatc accggcacta acgaatgatc attacacgag cagacctgcg
     7621 agaatggcgt atcggagcgg tgatgtatcg ctggttcctg cgccatttcc cacgcggcgg
     7681 tagttatgcc gatattcatc atgcactgat tgaggaagga tatacggact gggcggagag
     7741 tctggttgag tatgcgtgga aaaaatggct ggcggatgaa aacttcgcgc atcaggaagt
     7801 ttcctctatg caaaaactgg ccacagaccc cggagagaga gcattttgca gtcagtttgc
     7861 ccgcagcgac gatcatgctc gcatcggctg ttgtgaggat aacgccagaa tcgcaaccgc
     7921 tgggtatgct gcacaaattg ccagtatggg ttatagcgtg cggattggaa gtgtcggctt
     7981 taacagtcac attggcagct ccggtgagcg cgcacgggtt gctgtaaccg gcaattcttc
     8041 gcggataagc agcgctggag atagcagtcg gattgcgaat accggaatgc gagtacgcgt
     8101 ttgtacattg ggtaaacggt gtcatgtcgc cagtaatggt gacctggtgc aaatcgccag
     8161 ctttggcgca aacgccagaa ttgccaacag cggcgacaat gtacacatca ttgccagcgg
     8221 tgaaaattca acggtagtga gcacgggcgt ggtcgactca attattctcg ggccaggggg
     8281 cagcgccgca ctggcatatc atgacggcga aagagttcgc ttcgccgtcg ccattgaagg
     8341 tgaaaacaat attcgagccg gagtccgcta tcgtcttaat gaacagcatc agtttgttga
     8401 gtgctgatag ctttaccttt aatcaaacga aagacgccga cgagcacggg atgcaggctt
     8461 tacgaccgcc cgagccatcg cctcaccaat ttctgcacat gccgtaagtc cctgaacgaa
     8521 aggctggtcg tgcatcaacc agggcaacgc gccgaacgct acgcgcccac gaatatcttc
     8581 gggttgctgt aacgtataat cttcaccaac atcagggatt tcatcccctg ttttctgtaa
     8641 ttgttcgcgt agtccaggga aaggaatatc tttcacttta agcggacgct gcccgcgggc
     8701 atcaataaaa acgtcaaacg agtagctgtt gtcttccgtt ttcaggacgg tgcgcgactc
     8761 gttaatttcc attttgtagt cttcaccgag agcgagaata tgaatgattc ccgcttcacg
     8821 taaggcaagt aggcgacgaa tagactctga agggattgcc gcataattat cgatgaaaac
     8881 ccgggcaagg cctttactga accgtttatg gtcgtgttca ttcagatgtg gaacaatttc
     8941 ctgtacggct tcatgcaggc gcagaatgac ataacgccag ggaacagtat gtttttctcg
     9001 tttattgcgt tcgacttcct ggagattttt ttctgcccag tcaaattggt cgcgttgttt
     9061 gcgctcggca aaccaggctt gagcaaagga atcgacattc aggctctcta aggctatgcg
     9121 ttgactccag tctggatcag caaacttgat ttcctctact atcaatctaa atacccgatc
     9181 caaaaggcca tattcgcctt tttgaatctc agcatttaat gcctgatcag tgacgatgtg
     9241 taagggctcg tagggaatag ggcaatagaa atcggcttcg ggtaaaatac ccgtgcgcga
     9301 cattaacgta atatttagct tttcacttgc attatcgcgg tgaaaaacga cgtgttgttt
     9361 atcatcttca atgaacgaac catgctgaat agccactgcc attgccgcat ccagtccgct
     9421 caaggatgtt cccataatac ccacgttaca cgcatcgacc tttgcttcca tcaagcctga
     9481 ccacgggctt ggaaaatacg ttcgggttgc ttcttcttca tcaggccaga cgtgacccgt
     9541 ggcgatcacc gctaaatcaa acgtctcgct gggtaaatcc tgatttgtag cgagcatgac
     9601 gccagcattt gtaatttgca gatcggtaac ctggcatgat tcataaacag ccactgcaaa
     9661 tttttgcttt cgtgcctggt ctactaatcg taaaaattga tcgcggaaat attcgcccag
     9721 cagaattcgc ggtaaaaact gacgatcgtg caaggtttct tttttaacgc cataacgctg
     9781 gagatgactg gcttcttgct tttgtagcca ttcgagatac gtacaataaa tcggcggtat
     9841 ttcaatactg gcaatatttg ccagcatcat ttttgagttt tcctcatcac tgtatggcat
     9901 cccgacaccg gcctcgtcag cctgctcgaa gatagaaata gaaagtggag tttgttgctg
     9961 tagaagcgag aataaggtgt agatccccgt aggcccggca cccacaattg caattttttt
    10021 cattgttact tacctgtacg cattctttcg aatttagtgt tatcagataa gtttggttga
    10081 gatttttaga atttgagaaa tttcagacag ggatgaaaag ggcgctaacc gcccttgatt
    10141 aaattctgtc aggcgagttt tgcctgattg atttgactca gtggctcatt gacatcgaaa
    10201 actttggcga caacttcctc aacgccgagt tttttcagtt gggcagtgtg tttttccaga
    10261 taggcaaggg cgcttttttc atcagtgaaa aggtagatcc caccggcttc gtggttcttt
    10321 tcactttctg tccatacctt ccacagaaaa ccaggttcct gattaatcga ctctgcaagt
    10381 ggtttaagct gctcagccat tgcgtcgcca aatgggccat taaaagcaaa atgaagttgt
    10441 aacaaagttg ccatagcgat tctccttctg ataacgcata cgaagacagt gtaaatcttt
    10501 taacgtattg gatacagaga agaagcagag ggaaaaagga gaagaccaga ttaacaaaat
    10561 ctggtgcgtc cgagtggact cgaaccaccg acccccacca tgtcaaggtg gtgctctaac
    10621 caactgagct acggacgcag gatggtgcgt tcaattggac tcgaaccaac gacccccacc
    10681 atgtcaaggt ggtgctctaa ccaactgagc tatgaacgca acgttgtagg tgacaacggg
    10741 gacgaatatt agcggcagag tgggaaggtg gcaagaggca aaacgtaatt ttctgcgcta
    10801 tttcgaccgt ttgcagagct tttaagcaaa ttggctatat tttgttgatt tgcaagggcg
    10861 atttttattc aggatcgcat ttacatctga tacaacccaa taattcgtgt atcagagttt
    10921 aaacagagtt gtttgccacc atctcaaatc tgtcgtggat aaatcctccg tagcctttac
    10981 acttaggtag caataaaggc acacagatgc agcaaaggaa taaatcatgg gatctgatgc
    11041 gaaaaacttg atgagcgacg ggaatgtgca aattgttaag actggcgagg tcattggcgc
    11101 gacgcaactt actgaaggcg agttgattgt tgaagctggc ggaagagccg aaaataccgt
    11161 ggtcacgggg gctggctggt tgaaagtggc aaccggtggg atcgccaaat gcacacagta
    11221 cggtaacaat ggcacgctat cggtcagcga cggtgccatt gccacagata ttgttcagtc
    11281 cgagggaggc gcaattagtc tctctacgct cgctacggtt aatggccgcc atcccgaagg
    11341 tgaattcagc gttgatcagg gttatgcctg cggtttgttg ctggaaaatg gcggtaacct
    11401 gcgtgtactg gaaggacatc gcgcggaaaa aatcattctc gatcaagagg gcggcctgtt
    11461 ggttaatggg acaacctcag cggtcgtggt agatgaaggt ggtgaattgt tggtgtatcc
    11521 aggtggggaa gccagcaatt gtgagattaa tcagggcggc gtttttatgc tggcggggaa
    11581 agccaatgat acgttgcttg ctgatggcac catgaataat ctcggtggtg aagactctga
    11641 cactattgtt gagaatggag ccatctatcg tctggggacg gatggtcttc agctctacag
    11701 ttccggtaag acgcaaaacc tgtccgttaa tgtgggtggt cgggctgaag tgcatgccgg
    11761 tacgctggaa aatgcggtaa tacaaggtgg aacagtgatc ctgttgtcac ccaccagcgc
    11821 ggacgaaaat tttgtcgtag aggaagatcg cgcaccggtt gaactgaccg ggagtgttgc
    11881 attactggac ggcgcttcaa tgattattgg ctatggcgca gatctgcaac aatcaacgat
    11941 tactgtacag cagggcggtg tattgattct cgacggcagt acggtaaaag gtgacagtgt
    12001 cactttcagt attggtaaca tcaatctgaa tggcggaaaa ctgtggctga tcactggtgc
    12061 ggcaacgcat gtgcagctga aagtgaaacg cctgcgcgga gagggagcga tttgcctgca
    12121 aaccagtgcg aaagaaattt cacctgactt catcaatgtg aaaggggaag ttaacgggga
    12181 tatacgcgtt cagataacag atgccagtcg gcaaactctg tgtaacgcac tgaaactaca
    12241 gccagacgaa gacgggattg gcgcaacgct ccagcctgcg taaaaacaca ggcgcacccc
    12301 gtgaaggttg cgccttgtct ttattagcgg gatgctcgtt gcagaatgat ggctgacggc
    12361 agacgttgca ggtaccgcat acgcagcatc atcataatgg ctgccgacgt caggccaata
    12421 ataaagccta tccagaagcc tgctggcccc ataggttcaa cgaccagatc ggtcagtgcc
    12481 agaatatagc cgcttggcaa gcccagcacc cagtaagccg taaaggtgat atagaaaatg
    12541 gaacgcgtat ctttataacc acgcaaaatc ccactgccaa tcacctggat tgagtcagaa
    12601 atctgatata ccgccgccag caacatcaaa tgcgccgcca gcgttacaac ctcgggattg
    12661 tcgttgtaca acagggcgat ttgctcccgc agtgaaaccg tgaatatggc cgtcagggtt
    12721 gccatacaga cacccaccat aagcccggtc cgcgcagcgg tttgcgcatc cagcgttgag
    12781 ccctgaccca gacgataacc tacgcggata gttaccgctg ccgccagcga cattggaagc
    12841 acgaacatta gtgaactaaa gttcagggca atctggtgtc ctgcgacatc aacaataccg
    12901 agcggagaca ctaacagagc cacgacggca aacagtgtca cttcaaagaa tagtgccagc
    12961 gcaatcggca aaccgagttg aatcagtcgt ttcataaccg caggatcggg tttcgcggtg
    13021 cctttttcgt tacgaatatc gcgcatcgag cgggcgcgtt taatgtaaga aaccatggca
    13081 aggaacatga cccaatacac cgccgcagta gccacgccac aaccaacgcc accgagctca
    13141 ggcataccga aatgaccata aataaagata tagttcaccg gaatgttcac cagcaggccg
    13201 ataaagccca ttaccatacc cggcttggtt tttgccagac cttcacattg gttacgggca
    13261 acctggaaga aaagatagcc cggcgcgccc cacagcaacg cacgcagata acccacggct
    13321 ttgtccgcca gagccggatc gatgttttcc atggagcgga taatgtaacc tgcattccac
    13381 agcaccagca taatgagaac ggaaacaaaa cccgccagcc agaagccctg tcgcacctga
    13441 tgcgcaatgc gctcacgtcg accggaacca tttaattgcg cgataaccgg cgttaatgcc
    13501 agcagcagtc cgtgaccaaa gaggatcgcc ggaagccaga tagaagtacc gatagcgacc
    13561 gccgccatgt cggtggcact atagccgccc gccatcacgg tatcgacaaa acccatcgca
    13621 gtttgggcga tttgcgcgag aatcaccggg attgctaatg ctaataacag acgcgcttca
    13681 ctgatatact tctgcacgtg aacacctttt atttgtagtt atatgagaga ctaaaaagcc
    13741 gcctgaatag gcagcaaaaa gaagaagcag gggaaattca gtctattgta gcgaggattt
    13801 attaattctc cagtgaaaaa atagccattc ttaccccttc actggtaagg gcgttttcca
    13861 cctgctattg tgctgaacag aatgtcttaa ctgatttcag gagttgtaag tatgtttacg
    13921 gggattgtac agggcaccgc aaaactggtg tcgattgacg agaaaccaaa ttttcgcaca
    13981 catgtggtgg agttacccga ccacatgctg gacggtctgg aaaccggtgc ctccgtggcg
    14041 cataacggtt gctgcctgac cgtgacggaa attaacggca accatgtcag ttttgacctg
    14101 atgaaagaaa cgttgcgcat taccaatctt ggcgatttaa aagtggggga ttgggtaaac
    14161 gttgagcgtg cggcgaaatt cagtgatgaa attggcggac acttaatgtc aggtcatatt
    14221 atgaccaccg ctgaagtggc gaaaatatta acctcggaaa ataatcgcca gatctggttt
    14281 aaagtccagg atagtcagtt gatgaaatat atcctgtaca aaggatttat tggcatcgac
    14341 ggtattagcc tgaccgtcgg cgaagtcacg ccaacgcgtt tttgcgtcca tttaattccg
    14401 gaaactctgg aacgcacgac tcttgggaag aaaaaacttg gcgcacgcgt caacattgaa
    14461 atcgatccac aaactcaggc agtggtagat acggtagaac gtgtgctggc ggcacgagaa
    14521 aatgccatga atcaatcagg tacagaagcc tgatctgaaa tcgcccccgg agagggggcg
    14581 atagaatagc ctttagcgag cgactcgaag gccgttttca acaccgcgtg agaacaccac
    14641 ctgccagagc tgaatatcac gggcgcggaa agcacctgcg caggcattca gataataggt
    14701 aaacatccgt ttaaagcgtt cactgtagtt atccgcaatt tctggccatg cggcgacgaa
    14761 tcgttcatac cacgccatca acgtagtatc gtaatcagca ccgaagttat gccagtcttc
    14821 catcacaaag tggggttcgc tggactgagc aatctggcgt acagagggca ggcaaccgtt
    14881 cgggaaaata tatttattaa tccagggatc aacattcaga tcggtttttt tcgaaccgat
    14941 agtatggagc aggaatatgc cttccggttt caaattacga tccaccaccg taaaataggt
    15001 atcgtaattt ttcggtccga cgtgctcgaa catccccaca gaaacaatac gatcaaactg
    15061 gtcgttcagg tcacgataat cttgcagcaa aatggtgaca tccaggcctt cacagcgttc
    15121 ctgagccatt ttttgctgtt cggcagaaat ggtgacgccc accacgctta cgtcataatt
    15181 agatgccatg tagtgtgcca gtccgcccca gccgcagcca atatccagta cgcgcatccc
    15241 aggttttaac tgcaattttt cacaaatcat tttgagcttc gcctgctggg cagattccag
    15301 attatcggca tctttccagt aagcgcagga atattgcatg aagggatcaa gcatgcggct
    15361 gaacaagtca ttacccaaat cgtaatgctc tttgccgact atccaggcac gttttttact
    15421 ctgcagattg aagagacggg cgccggcaat acgcagcgtg tctttgaaat gatgggggag
    15481 ttggttctcg agacctgcgc gtaagacttt gctaaaaaac atatccagtc ggtcacattc
    15541 ccaccagcca tccatataac tttcgcctaa ccccaaagag ccttcttgca gaacgcgttt
    15601 aaaaaaatcg gggtttttca cacgaatatc cgccggggca gaaccgttaa tggctatacc
    15661 ggcacggcta agtaattcgt tggcgatacg gtaccagttg tcatccggta cactgacttc
    15721 ttctatacac gatgaactca tagtttctcc atcactggtc ggtgatcaga accttaaaac
    15781 agcgtagacg ctttttgggc tttgtgagaa atctcgcaga aaaaaccgcg agtctataat
    15841 ccgacgtaga acagagggaa aggaggaacc cttgccgaaa tgaccatcca tagtgagtcg
    15901 ggaacgatcc tgtccccgta aaaacataat atttatcgta gctttaacaa ctcgaaatta
    15961 gtataggccg ctaatttcaa tcatacaaca aaattttgtt agcagcctaa gtataagtat
    16021 atcgatatag atcagtgtga ttcgctatga gcgacttcgg cattgccatg attctggcag
    16081 ccaacttctt cacaacgttg catcatgtaa cccagcgcga ccagcactac tgttgataac
    16141 atcacgctgg tggtggtgag caatggcgtg ctgatactga tcagccagga aactaccaga
    16201 cttgcgagga agcacagacc cagttgcaga gtgttctgca acgctgcggc gcgaccagtt
    16261 gcgtgtggga agggacgcag agcctgggcg acaacaatag ggtagatcgc gccattagca
    16321 atcgccatca cacagaatgg gatcaggatt tcgaccagcg acacatggct aatgaagccc
    16381 gcagcccagg tcgcaatgac gctgacagca aacagcacca gcaaccacgg taataactgc
    16441 ttgccttgcc atttctgcag cgcggcgcga cagccataac caccaatcag aaacgcgata
    16501 gtttgcggga cataacttaa accaataact gccgggctgt agcccatttc actaaggatg
    16561 aacggtgaac cggtcagcca tgcaaaaaaa ctggctgaac aggccgcgta tatcagcaca
    16621 ttgccgcgat aggttttaga acgtagcagg tcggtaaagg tcagaccatc ctgactattg
    16681 ttacgggcct tcgtcgtggg tttgagccag aaaataggca gaatcagcac cacggtaatg
    16741 gcaaaaaggg tggcgaaaat cgcctgccag gaaaaatgga ccagcagcca gcttcctaac
    16801 agaggagcca gggccggaga tagacccacc agcggcatga tggtcgcaaa aattcggtta
    16861 actttctgtg aaggataata atccgtcact aacgcttgcc agataaccgc cgcggcgcag
    16921 acacccacag cctgtacaaa acgcaatacc agcagcatag cggcgttttc tacccacagc
    16981 atccccagac tacctaacgc aaaaattgtc aggccgatta ataataccgg tttacgacca
    17041 taacgatcgg agagcggccc ccacagaagc tgggctgcgg caaaaccggc aaggaacaga
    17101 ctaaggctgg cactgacagc agacgcaggc gtttgcaggt cggcctgtat ggcggcgaaa
    17161 gcaggcagat acatatcggt tgccagaaaa ccgagtacgc tcaaacccgc cagccagact
    17221 aaaaatcttt tcccaggttg catgtatatt ctctaaatct ctcaattaaa caccgccgca
    17281 aagtgtaggg agtgaaatta gtgttgtgaa acggtaatat ttgatgactg gtttcaaaaa
    17341 ttttgcaggc agactatgtg gtcagaatat tcactcgaag ttgttgatgc ggtagcgcgt
    17401 aatggtagtt ttagcgctgc ggcacaggag ctgcatcgcg ttccttctgc ggtcagctat
    17461 accgtgcgtc agctggaaga gtggctggcg gtgccgctgt ttgaacgacg gcaccgtgat
    17521 gtggagctga ccgctgccgg agcgtggttt ctcaaagaag ggcgctctgt tgtcaaaaaa
    17581 atgcagatca cccgccagca atgtcagcag atagcgaacg gctggcgcgg tcagttagct
    17641 atcgcagtgg ataatattgt caggccagaa cgtacacggc agatgatcgt tgatttttat
    17701 cgccattttg atgatgtcga acttcttgtc tttcaggaag tgttcaacgg tgtctgggat
    17761 gcgctttccg acgggcgcgt ggaactggcg attggcgcaa cacgcgcgat tccggtaggc
    17821 ggtcgttatg ccttccggga tatggggatg ctaagctgga gttgcgttgt tgccagccat
    17881 cacccgctgg cgttgatgga tggcccgttc agcgatgata cgttgcgcaa ctggccgtcg
    17941 ttggtgcgcg aagacacctc gcgaacgcta cctaagcgta ttacctggtt gctggataac
    18001 caaaaaagag ttgtcgtgcc tgactgggaa tcatcggcaa cctgtatctc ggcaggatta
    18061 tgcataggga tggtgccaac acatttcgcc aaaccgtggc ttaatgaggg gaagtgggta
    18121 gcattagaac tggaaaaccc ctttccggat tcggcatgtt gtctgacatg gcagcaaaat
    18181 gatatgtcgc ctgcgttaac ctggttgctt gagtatctcg gcgatagtga gacgctgaat
    18241 aaggagtggc tgcgggagcc ggaagagact cccgcaacgg gtgattaacg acgatagtcg
    18301 cggaacgggc cgtcagccac ggagcggcgt tcaatcaagc gcggatgcac ttcaatggac
    18361 tgcggttctt cacgtttgtt gactatacga tccaacaaca tgttgaacgc tgtttcaccc
    18421 aacgaatctt ttggctgatg gattgttgtc agcgccggcg taaaatagcg ggcgttgcgc
    18481 acgttatcat aaccgatcag cgaaacatcc tgcgggacgc gcagccccat ttcatcagca
    18541 gcacaaagtg cgcccattgc catgatatcg ccaccacaga agacggcagt agggcgatgc
    18601 ggctgcgaca ggatttgctg catggcgcga taaccggatt caggttcaaa gtcaccctgc
    18661 acaatccagc tttccggcac cttgatcatt gcttcttcca tcgccttcat aaaaccggca
    18721 aggcggcctg cgccagtgtt acgttccagc gggccgggga taacgccgat ttcgcggtga
    18781 ccgcgttcaa tcagataacg cccggccatg tagccgcctt cgaacgcgtt atcaatgacc
    18841 gcatcggtga agtcagcttt tgcttcaccc cagtccatca ccaccattgg gatatggcga
    18901 tactcttcca gcatcgccag caacggctct gggtactcag aacacatcac cagcagacca
    18961 tcgacgcgtt tttgcgccat catcgacaga taagcccgct gtttctcaag attgttccac
    19021 gcattaccca gaatcagggt gtaacctttc tggaagcaat ttttttcaac tgcttcaatg
    19081 atctcggcaa aataggccgc ttcgctgctg gtcgctagca aaccgataga cttggtgtgg
    19141 ttaaccttca ggctacgcgc caccgcgcta ggggagtagt gtaattcttt gatcgctgcc
    19201 cacacggcgt tgcgcgtttc ttcagcgacg aaacgtgttt tgttgatcac gtgtgacaca
    19261 gttgtagtgg aaacgtttgc tcgtttcgct acatctttta ttgttgccat tccatttcac
    19321 tccagaccct aacttcagct cctgcaaaat cgcaaggtaa acgtttgcct ttacacacct
    19381 tacgcacaac aaaagtgttg cggtacgccg gaaaaacgac acggagcgtc aggaaggggt
    19441 caatggccag tacgcaaata gtgtaagcgt ggaattttgg ccgatcttga cgaaaagggg
    19501 aagtggtaaa aacacttatc attataaatg acgggagatt ttttcatcac agtgtgtaaa
    19561 aatgcgtaat atcacttttt gtgtggtaat aaaaaaggag aaaacttgat gagcaccgac
    19621 cttaaatttt cactggtaac aacgattatc gtcctcggtt tgatcgtggc cgtgggtttg
    19681 actgccgcgc tgcactgatt tcctctcgag ggagcgcgtt ctccctcgca tctccttcct
    19741 gtttgttagc aaaatggcaa aagtgttttc tctttttgtt attttcattt ttttgtgatt
    19801 gctgtcatgc tttcgccact ttaatgaata tcgcgtaatg acgcgaagag gtggagataa
    19861 ggcatgaaaa ttaactatcc gttgctggcg ctggcgattg gcgcgtttgg tatcgggaca
    19921 acggagttct cgccaatggg cttgttgccc gtcattgcgc gcggtgtgga tgtctcgatt
    19981 cccgctgccg gaatgttaat cagtgcctat gcagttggcg taatggttgg cgcgccgctg
    20041 atgacgcttc tactttctca tcgtgcccgc cgcagtgcgt tgattttcct gatggcaatt
    20101 ttcacgctcg gcaacgtact ttccgccatc gcgccggatt atatgaccct gatgctttca
    20161 cgcattttga ccagcctgaa tcacggagca ttttttggtt tgggttcagt cgtggccgca
    20221 agcgtggtgc caaaacataa acaggccagc gcagttgcca ctatgtttat ggggttaacc
    20281 ctggcaaata tcggtggcgt gccggcggcg acctggttgg gtgaaaccat cggctggcgg
    20341 atgtcatttc tggcaacggc ggggctggga gtgatttcaa tggtaagtct gttcttctca
    20401 ttacctaaag gtggtgcagg ggcacgacct gaagtgaaaa aagagctggc ggtattaatg
    20461 cgtccgcagg tgctgtctgc attgctgacg acggtactgg gagctggtgc aatgtttact
    20521 ctctacacct atatctctcc ggtactgcaa agtattaccc acgcaacacc ggtgttcgtc
    20581 acggcaatgc tggtgctgat tggtgtcgga ttctctatcg gtaactatct cggcggcaaa
    20641 ctggcagatc gttcagttaa cggcacgttg aaaggctttt tgttgctgct gatggtgatt
    20701 atgctggcaa tcccgttcct ggcccgcaat aagttcggcg cagctattag catggcggtg
    20761 tggggcgctg caacctttgc ggtcgtaccg ccgttacaga tgcgcgtgat gcgtgtcgcc
    20821 agtgaagcgc caggtctgtc ttcatcagtc aatattggtg cctttaatct tggaaatgcg
    20881 ctgggagcag ctgctggtgg tgcggtaatt tccgctgggc tgggatacag ctttgtgccg
    20941 gtgatggggg cgattgtcgc gggactggca ttattgctgg tgtttatgtc agccagaaaa
    21001 caacctgaaa cagtttgcgt tgctaacagc taattaaaca aaaacgcaga aggataacct
    21061 tctgcgttac agttgctgac aacgtgcgat ttatcttgcc gaatgcgctg ttaacgcctc
    21121 atccgcgttg gcatggtaag cgaagcgcat caggcaatac tgcatttgcc atcagttatt
    21181 atgcagcgag atttttcgct acgaattccc agttcaccag cgcccagaag tgctcaagat
    21241 agccaggacg tgcattgcga tagtcgatgt aatatgcgtg ttcccagaca tcaacggtta
    21301 gcagcggagt cgcatcggtg gtcagcggag tacccgcgtt agaggttgaa acgatagcca
    21361 gtttgccatc gctgtttttc accagccagg tccagccaga accaaagttt ttgatcgctg
    21421 catcagtaaa ctgagctttg aaatcggcaa agctgccaaa agatgcggcg atagcttcag
    21481 cgacttttcc agtcggttcg ccaccggcgt tcggtgccag gcagttccag tagaaagtat
    21541 ggttccagac ctgagctgcg ttgttgaata cgccaccttc agagctgcga ataatctctt
    21601 ccagtgattt accttcaaac gcggtacctt taatcaggtt gttcaggtta gtgacataag
    21661 tctgatgatg cttgccgtag tgatactcga tagtttccgc agaaatgtgc ggtgccagag
    21721 catctttagc atatggtagt gcaggtaatt cgaatgacat tgctactctc ctttattatt
    21781 aatttgcata cgtacaatag ccttattgtg cgtatggata gggtagcaaa ttaaaaggtg
    21841 agacaaaaga tgaacttacc ctgttgccgt aacaacaggg taaagtttta gcgaagtgtt
    21901 tttggggtca ttacccggcg agcgccaaca tagtggcgct gccagtagtc ttcactgaga
    21961 gaagtgattt gaatttcctg acctgtgcgc ggtgactgaa taaatttgcc gttgccgaca
    22021 tacacgccga catgatcggc tgtaccgcgt ccctgagtac ggaaaaagac caggtcgccg
    22081 tttttcagtt cactacgttc gattggcgct gcatcgcgca ggtgatacat ttcattcgcc
    22141 gtacgcggaa tacgaatttt caccaaatct ttataagcgt aataaaccag gccgctgcaa
    22201 tcaaaaccgg tacgcggtga gctgccaccc caacgatatg gcttaccaat ttgctgcatc
    22261 agtttattca ttgccacttt tgtggctttt tgcaccttcg ctttgtgtgc gtcggcaagg
    22321 gttcctgacg ctgcattttt gactttcaca caatgcgatt tatagccctt acgtttggta
    22381 cacttttcgg taaagctggc tgaggccgtt ttattgacgg tatgtttgct gcgagttttg
    22441 gcgtttttag aagcggtaat ggaagatttt ttaacggaag aagcggtttt gcttttggtc
    22501 gtacttttac tggcggcttt tttcgctgtt ttctgggtct ttttggtggt tgccgtgctt
    22561 ttctttttat ctgctttttt ggtgatatga gtggtagcag aactctccct ggcttgcttt
    22621 gaagcatggg ccataggcgt taaaggcagg gtggtaaaaa gtaaagcaca gagcgtgatc
    22681 gaaatacggt ttatccgcgc cactgagcgt ccctctgtta aggcaggtct atcatgcctg
    22741 cgtctgattt acaaaacttg atgattctaa tgcatagaag aagagaaagt taatagcttt
    22801 tattgttaaa aaatacgttt cgttggcgag cgcaaaaaaa tgcagcagtg aaagctaaaa
    22861 atggtcataa atacgccagt ttgccgtgca tcagggaaga aacgaatatt tgaaatgcaa
    22921 caaatagagc aaagggataa aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg
    22981 cactgacccg ctacaatgtc agattactgc cgtaaagaag tttgaggaag caagacaatg
    23041 agcaccacta tcgaaaaaat ccaacgccag attgctgaaa acccgatcct gctgtacatg
    23101 aaaggttcac cgaaactgcc gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc
    23161 gcatgtggcg aacgttttgc ctatgttgat attctgcaga atccggacat tcgtgcggag
    23221 ctgccgaaat atgctaactg gccgaccttc ccgcaactgt gggttgacgg cgagctggta
    23281 ggcggttgtg atatcgtgat cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa
    23341 gaaactgccg ctaaatacaa gtctgaagag ccggacgcgg aataatccgt cctgcaatac
    23401 tgacggatgc gaacgggcat ccgtcagtcc tttctctatc cccaatccag cccttgtggc
    23461 gaacttgaaa atcccgcttc acgcagcagc gtaaacatcg gcgtttgcca gaccggtaga
    23521 tcattcactt ctgttagcgt aaagcgcagc cgtggttcgc gacgcagtgc ggtagtcagc
    23581 gcgtggaaaa cctctggggc gagtaattcc tctttttcct gccagaccag catttttttg
    23641 ccaccttgcg ccagatagag taacaatttg ccgccagaaa cgaccaccag cgcacccgcc
    23701 cgacgcgttg gtaccagcgt tgccggatgt gcaggccagg gcagaagatt tccccacaca
    23761 ttagccggat cgttggcgga gagcgccact ggtgtatagt ggcgcgtttg cgcggcttgt
    23821 gtcgccaggt cgcgcaagcg gtcaatagtc agacgttcag cgaattgcgc gccacccaga
    23881 ccttctacaa aacgacctcg cataattcgc ccggaatctt ccatacttcg acaaagcgtt
    23941 tgcatcgatg gaaacccgcc agggatattt tcggctatca ccgcctgacg actgatgatg
    24001 ccgtagcggt cgagcatatt ttccgccagc gccagcatcc tttcggtatc gtttagtggc
    24061 tccacctgca ataacgacca gcgtccagcc agatttggtg tgttgtaaga tacccgcggc
    24121 gagacgggtt gtgcatagac aggacgccca cgacgagccc ggtgactgcg gcgagttgag
    24181 gtgcgtgcgt tggaactgct gcgggtgagg gcgcgtaacg gtgcccaaat atcgctggtg
    24241 atgacgcctt gccagaccag cgcccataac gcctcttgca gtgccgaggg atcaaccgat
    24301 tcgccgattt tgtcgcgtat ccgttggctg atttgttgtg caaaccaggc tcctccgtca
    24361 gccagaacag cgactatcgc ttgttgcagc gccgaacgat tcgcctgatc cgcttcggcg
    24421 ggagtgaacg attctgcagc atattcctgt agatgcagtg ccaccaggcc gtcatcttca
    24481 cccagttttt tttgccccga ccagataacc gcaccggttg ccagtaattc atcgagcact
    24541 tctggcgaat agtcgtgtac gcgggcaggc aagatctggc tttcccagag tgaggcgggt
    24601 aagccgactc cggcaagctg ttcgatcacc cgcatcacgc catccacccc ctcataaacg
    24661 cctggcgatg ttgaggcaaa gagcgccggg ctaccatcgg tggcgggtaa tacgccctga
    24721 cgttccagca gcaatcgcgc ataggtcgtg gctgcaacgg gacgcgtcgc ttctctggcg
    24781 gcttgcagcg agcgcaaacg cagacgacga aatacttcat cgctcaccca gatgtcttgt
    24841 tgcagattca tcaccagacc ctgttcgcgc agttgctgaa gctgctcttc gacaatggca
    24901 ataccgagac taaactcatg agccagttgt tcagccgtga ccagagcatg agcccggaga
    24961 tagcgtagaa agaggtcgcg aagcgggtaa ctgactctat gcaaataaat ctctggcagt
    25021 gactctggta atcgtacgcc gagggcatcg cgcagcctgg cggcatcatc catacaggca
    25081 agacgctcct gtccactaat catcgtcggg aagatgcgtt ttactgtaag aagattttcc
    25141 agatacgagg caatctcttc actgctgcct gtatgccgtt gcgccaggtc ttcaacggtc
    25201 attggcccca gttcgcgcag caggtcgaac aatccttctt cacctttcgc tcttctgcca
    25261 ggagccagtc gttgcaactc ttcttccacc tggcgaatga cctgcgggtc gagtaattcc
    25321 cccggatcga cctgtccgag tagattgcgc agtaactcgc tgtccagcga cagtacggat
    25381 gcccggcgtt ctgccagcgg ggcgtcgctc tggtacataa attccgcgac atagccgaac
    25441 aataaacttg tggcgaaagg cgagggcgta gtagtcgtta catcggatat ttgaatttcg
    25501 cctccgttca ggcgacgcat caaacgttcc agtgcgggaa gatcataaac atcttgcaga
    25561 cattcgcgta gggtttcgag aatggccgga aaatccggat atccctgagc gatttccagc
    25621 aactgactgg cgcgcagccg ttgttgccaa agcggggtgc gatggcccgg cgtgcggccc
    25681 ggcattaata atgcccgcgc ggcgcattcg cgaaaacggg cggcgaaaag tgccgaactg
    25741 cctaccgcct cgcggacaat ttgcagcaac ttttctggtt caaacaaaaa aatcgcggcg
    25801 tcgggcaatt taccatcagt gtcaggaatg cgcgcaacaa tgccgtcatc actggcgacc
    25861 acagacgcgt cagcgcccca aagcgcatgt attcgcccgg caatcgccag cgcccagggt
    25921 tcatgcaccc gtcttccata gggagagtgc aaaataatac gccaatcgcc aatttcatca
    25981 cggcaccgtt cgaggaccag atgacgactc cccggaacaa tacccgtcgc gttgcgctgc
    26041 tcgtcaatca acccctgaat attggcgatc gtattttctt cagccagcca cgggggaata
    26101 gtcccggaaa agaacgctcc atccgccagc aaatgaagaa aatcgccgat catctcgcct
    26161 aattcagccg gacgtccgtt accttcacca cgccagaagg ggagccgggc agaacgaccc
    26221 ggagcaggag tcacaatcac ctgatcgcgg gtgatttgct ggatccgcca tgaggttgcg
    26281 ccgagagtga taatatcgtt cacccgcgac tcatatacca tctcctcatc cagttcaccc
    26341 acccgtcgcg aaccggcctg ttcttcccct tcgggtaata acacgctata catgccacga
    26401 tccggaatgg tgccgtcgct ggtaaccgcc aacagctgag cgccaggtcg ggcggtcaat
    26461 atcccggtct ccctgttcca gaccagtttg gggcgaaaag cagaaaaatc gccagaggga
    26521 tagcgtccgg aaagcatatc cagcgtggcg tcaaaaacac gtcttggcag atctttccac
    26581 ggtgcggcac ggcgtacgcg ggagtaccat tcgtctacct gtaatgcatc catcgccgcg
    26641 gcggcaacgg tgtgctgcgc aaggacgtcg agtggattat gcggaggtgt caggttttcc
    26701 agcctgcctg cgaacataca ctctacaatg actgcggaat cgactaagtc acgcctggtt
    26761 cgggggaaaa aaagcccttt ggagatacct cctatctggt gtccggcacg tccaatgcgt
    26821 tgtaacccac tggcaacaga aagcggcgtt gccacctgaa tcaccagctc cacagcgccc
    26881 atatcaatcc ccagttcgag actggaagtt gcgaccacgc agcgtaactc acccgatttc
    26941 agcgcctgct cggtgattgc tcgttgttct ttagagacgg agccgtggtg tgagcgggca
    27001 ataaaaacgt cgctactttg tacacggtta gaggttgcgc cagaggtcga ctcgaaatgg
    27061 gccgcatcag cggcgataga cggggaacgc tgtaagcgtg cggcgtaaag ctcatttaat
    27121 cgtgccgtca gtttttccgc cagcccccgc gaattagtaa agacaatggt cgagcgatgg
    27181 cgcaacactt catcaaggat acccgtttca atatatggcc agatggagcc ttcccggccg
    27241 gcatggctgt cttcgccagt gccgctggcg accgatgaga catcatccat attggcgact
    27301 ggtacgacaa tccgtatctg cggatgacgc attgcgggcg ggttgactac cgtaaccggg
    27361 cgatcgccac caagaaatgc tgccacatcg ctggctgagc gcacagtggc agaaaggcca
    27421 attcgctgtg ctgaggtgtg gagcagcgca tcgagccgct ccagacttaa cgccagatgc
    27481 gcaccacgtt tacttcccgc taccgcgtgg acttcatcaa taattaccgt ttcgacgccg
    27541 cgtagcgttt cgcgcgcgcg ggaggtcagc atcagataga gtgattcggg tgtggtgatc
    27601 agaatatccg gcggattacg ggtgagtttg ctgcgttcct gtgcaggcgt atcgccagta
    27661 cggatcccca cgcgaagatt gacttccgtt tcgccgcgcc gccgccgttc atcggcaata
    27721 cccttcaacg ggatctgcaa gttgcgctga acgtcggtgc ccagggcttt tatcggtgaa
    27781 atatagagga tgcgtgaggt tttacgttta tgcgcctcgc gggtatcttc gccgccttcg
    27841 cggaagagtc gatcgagggc gtagagaaat gctgccagcg ttttcccgga gccagtcggt
    27901 gcaatcacca gcgcatgttc gcctcgcgcc gccacatgcc aggtttgcga ctggacagcg
    27961 gtcggctgtt taaaggcgcg aagaaaccag tcgcgggtcg ccggtgaaaa cacgtccggc
    28021 aggggcgatg aagggtttgg attatctgcc attaacgcta ttccccgtga atatcatgca
    28081 tgatgcgtgg aatagcctca ctatagatgc ctgaatcatt gcatgtcatc aggcatcgac
    28141 tcgattacac ctcttcggcg gcaggtagcg gccagcctcc cagacgtttc cagcggttga
    28201 cgatttcaca aaacagcacc gcagtgcgtt cggtgtcgta cagcgcggag tgcgcctggg
    28261 tgctgtcgaa gtccatgcca gcggtctggc aagcctttga caatacggtt tgtccgagtg
    28321 ccagcccggc cagtgcagca gtgtcaaaag tggcgaaagg gtggaacggg ttacgtttca
    28381 gtgaggcgcg ttctgcggcg gccatcataa agctgtgatc gaaattggcg ttgtgcgcca
    28441 ccataatggc gcggttacag ccgctcgctt taataccttt acgtacaact ttaaaaattt
    28501 cgtgcagcgc ctcgtattcg ctgaccgcgc cgcgatcggg atcgttcggg tcaatgccgt
    28561 tgaaagcaag ggcttctggt tgcaaatttg cgccgacaaa tggttcgacg tggaaatgta
    28621 atgtggtgtc cggcatcagc cagccttgtt catccatttt cagggtgatg gcggcaatct
    28681 caagcagcgc atcggttttg gcgttaaatc cagctgtttc aacatcgatc acaacaggat
    28741 aaaagccacg aaaacggtcg cacagaccgg taagttgagc gttatcggac atctgattct
    28801 cttaatacgg ataaattgca gcgcgcatta tgacaaatat tacttgtcga tgcagtaaag
    28861 atgcgggcgc gatgagttcg cgcccggcag gagattagtt gcccagaccg cgaccggcgt
    28921 ctttctcttc gattaactca attttgtaac cgtccggatc ttccacaaac gcgataaccg
    28981 tagtaccgcc ttttaccgga cccgcttcac gggtcacgtt acccccgttt tgacggattt
    29041 tttcgcacgc ttcagcggca ttatctacgc taagcgcgat gtgaccataa gcagtgccga
    29101 gttcgtattt atccacgccc cagttgtagg tcagttcaat caccgcttct tcggtttccg
    29161 ggccgtagcc aacaaacgcc agtgagtatt tgtattccgg gttttcgctg gtacgcagca
    29221 gtttcatgcc cagcactttg gtataaaaat cgatggagcg ttgcagatcg ccaacgcgca
    29281 gcatggtatg aagaagacgc atctttttat cctcaaaatg gctaacagat tcaaaatgtt
    29341 gttttagtat agcggcggct ttacgccgct cgcaatgttg gattacaacg tcgggtaatc
    29401 agtgtagcct tccgcaccgc caccatagaa actttcagca cgctgtgggt taagctcagc
    29461 tttgcgctgc aagcgggcga ccagatccgg gttcgcaatc cagtcacgac caaatgccac
    29521 cgcatcaatt aaccctttgc cgatcagcgt ttcagctttt tctactgtgt atgcacctgc
    29581 gccgataatc ggaccgtgga aacgggcgcg tactttttcg cggaacgcat cagtatacgg
    29641 ttcacccccc gcccaatctg gttctgacat atgcagataa gcaatgccgc gtttacccag
    29701 ttgttcaatc agatacagtg catcggcttc ttcattcggg ccgttatccg tgttctggaa
    29761 agtaccgatt ggcgaaacgc gaatgccaat gcgatcggca ccccattctt caatcccggc
    29821 atcgaccact tccagtacca aacgtgcgcg attttccacg ctgccgccgt actgatcggt
    29881 acgatggttt gaagaaggag aaaggaactg atgcagcaaa taaccatgag cagagtggag
    29941 ctctaccaga tcaaaaccgg cttcacgcgc gttagcaata gcctgacgga aatcattgac
    30001 gatacttgga atctcttcca gttcaagcgc acgcggcatg gatgtttcaa cacggatcgc
    30061 ctgaccattt tcatcgcgca gagaagtacg tgttcccgcg ctaagtgctg aaggcgctac
    30121 tggtgcctgg ccgccaggtt gcaggctggc gtgagaaatg cgtccggtgt gccacagctg
    30181 tacggccata tgcccatttt cagcatgaac gccagcggta atttttttcc atgcggcaat
    30241 ttgttccgga ctatggatgc caggtgcacc agcataccct tttgcctggg cagaaatttg
    30301 cgtggcttcg ctaataatca aaccggcact ggcacgttgg cgatagtatt ccgccatcaa
    30361 cggggtaggg atgtcacccg gttcaatact gcgcagacgc gtcagcggtg ccataaaaat
    30421 acggtttgcc gcagttattg cgcccacttt cagtggggaa tacagttttt cagatgacat
    30481 aaaggctccc ggtcgtctgg tgataaaaaa tgcctgctaa acggcaggcg tcgcaataat
    30541 ggtttttaca tgtgccagcg cgttttccag tggctcggaa ttgcgcgaaa ttttggcctg
    30601 cagattcgca cccagccata gtgcgtaaag cacttgtgcc tgttgcagcg gttcgccaca
    30661 aaaggttaaa caatggctat cacggccatt ttccagagcc tgcgagagca gggcgatcac
    30721 gccgcgagcg cctttatcca tcgcgctacg catatcttct gacagatcgc acacttcggc
    30781 agagagtttt actgtcaggc aaccactgat ggttccatgt tggcaaaact ggttcagtgt
    30841 ttgctggtaa taagccagta tgcggtcgcg gtagttacct tcgccggatt gcagcaactc
    30901 agtcagtcgt tggtgatatg tggcgtaatg gcgctcaagc atggcaacgc caaacgcttc
    30961 tttagagcga aagtagtgat agaaggaccc tttcggcact tcagcggttt ttagtaattc
    31021 gcttagcccc atcccggtga atccacgttg caggcaaagt tgctcgcccg tcgccaggag
    31081 atgttcgcga gtatcatgtt cggtgtgttt gttcatgcgt tggagtgtag tagaccagtc
    31141 ggtctagtgc aagcaaagtt gctactttgg gcattaacag cttcaataat ggtaataatc
    31201 ttatttgagg agggcgaagt ggcggagcaa ttagagttct ttcccgtcca gagcccgtgc
    31261 cggggaattt gccagtctga cgaacgcggg ttttgtcgcg gctgttttcg aagccgggat
    31321 gagcgtttta actggaataa aatgagcgat ggcgaaaaac aagaggtgct gcgcctttgt
    31381 cggcagcgat taatgcgcaa attacgtgca aataagccag cgtcatcaga cgaaccggag
    31441 caaccatcac tcttttgagc ctaatcttgc gtatactcct ctcatatctg ataagaggaa
    31501 gcgattatgg ttcagcgtat tactattgcg ccgcaaggcc cggagttttc ccgttttgtg
    31561 atgggctact ggcgattgat ggactggaac atgtccgccc gccagctggt cagttttatt
    31621 gaagagcatc tggatctcgg cgtgaccacc gtggaccatg ctgatattta tggtggctat
    31681 cagtgcgaag cggcgtttgg cgaggcgctg aaactggcac ctcacctgcg tgagcggatg
    31741 gaaatcgtca gtaaatgcgg tatcgcgacg accgcgcgtg aagaaaacgt cattggtcat
    31801 tacatcactg accgcgatca catcattaag agcgccgaac agtcgctaat taatcttgcg
    31861 accgatcatc tggatctgct gttaatccac cgcccggacc cgttaatgga tgccgatgaa
    31921 gtggcggacg cgttcaaaca tctgcatcag agcggcaaag tgcgtcattt tggcgtatcc
    31981 aacttcacgc ctgcgcaatt tgccctgttg caatcacgtc tgccgtttac ccttgccact
    32041 aatcaggtgg aaatatcccc ggtacatcag ccgttactgc tggatggcac gctcgaccaa
    32101 ctgcaacaac tgcgtgttcg tccgatggcg tggtcctgcc ttggtggtgg tcgtctgttt
    32161 aatgatgatt atttccagcc gctgcgtgat gaactggctg tggtggcgga ggagttaaac
    32221 gcgggctcga ttgaacaggt ggtttacgcc tgggtattac gtttaccgtc gcagccgctg
    32281 ccaattatcg gctgaggtaa aattgagcgc gtacgggcag ctgtcgaagc agaaacactg
    32341 aaaatgaccc gtcaacaatg gtttcgtatc cgtaaagcgg cactggggta cgacgtaccg
    32401 taagcacttt taggaatagc cgccgttcaa aaatgtgtca ctggtttaca cttattcagg
    32461 aatgcacaat gaacggaggt cctatgaaac gttttagtct ggccattctg gcgctggttg
    32521 ttgcgaccgg cgcacaagct gccagtgaaa aagtcgagat gaacctcgtc acgtcgcaag
    32581 gggttgggca gtcaattggt agcgtcacca ttactgaaac cgataaaggt ctggagtttt
    32641 cgcccgatct gaaagcatta cctcccggtg aacatggctt ccatattcat gccaaaggaa
    32701 gctgccagcc agccaccaaa gatggcaaag ccagcgccgc ggaatccgca ggcgggcatc
    32761 ttgatccaca aaataccggt aaacatgaag ggccagaagg tgccgggcat ttaggcgatc
    32821 tgcctgcact ggtcgtcaat aatgacggca aagctaccga tgccgtcatc gcgcctcgtc
    32881 tgaaatcact ggatgaaatc aaagacaaag cgctgatggt ccacgttggc ggcgataata
    32941 tgtccgatca acctaaaccg ctgggcggtg gcggtgaacg ctatgcctgt ggtgtaatta
    33001 agtaattaag aggccagcgt accttgcggt ggtgcttgct caagttgcga aagcgagcag
    33061 tacagccgcc agacaattgc cgccagctca cgggcggcag gttgatgatg gcgggcaagg
    33121 ctgtcgcaaa tccgctgtaa ttcttcaagt gtggccgcca gtgatttttg ctgaacgcca
    33181 cgctcactca tcactccccg caacagtgaa atacagttat cccgtactcg cgataacgga
    33241 tcggaacgcg attcccagtc gcgtaattgc cagacaacat gagaacagtt cagcagcact
    33301 ataccccagc gtaataacca acggcgagcc agcgcatcct ggctgttact caactgactg
    33361 acgtgatgat aagtgagcga ttcaaattcg ctttcactca gtgttggatg gcggcttagt
    33421 tgatcgacaa aatcccggcg cagcgcgcga atatggcggc ggcttttgcg agcatccgat
    33481 cctggacgca gaatggcgaa cgctaaccac gccaacgcga cgccaacgat ttttgccaga
    33541 ttatcgttaa gaaaatcagc aaaattatac accgggggat tagtgacggc gataaaagaa
    33601 cccataaaaa caataagttg cccccacaat gcggcaaatt tgggcatctg caatttaaga
    33661 agctgcattg tcgccagcag tggaaagaga aacagtaaaa attgccacag atcgctaatc
    33721 tggaccatca gaccaaattt gaccacaaag ctgaatagcg aaagtaacac cagcgtgcgc
    33781 atcagcagtg acaacgactt aaacggtgct gcgacggcgg agtagagtac gcagctaatc
    33841 gctgccagcg ttaatgcatt ggcaccggca tcccattgcg aagcaatact ccatgcgcca
    33901 atcatcatca acgtacaaaa tgtacgcagt ccgctccaca tagcttcggc gttatcggta
    33961 tgacgagcca ggccggatgt ccgtgggagt ctggcatgat catctacatc gctttgcaga
    34021 cgatgtaatt cctgactact ttgcagataa aggcggcaaa aataacgtag tcgctgccag
    34081 aaggcgacgt gccgatagtc ggcgacgttg gtcgggcgta gcggggcgat aatacgtgcg
    34141 acggtgtaaa catctgtttg cgaactggcg agcgccgtca gcaactgttc gagaatttct
    34201 cgcgtggcac ctggcggtga gggccagttg agcaacatac gtcgcaggct ggagatgaca
    34261 ctggtcatac gtaattgctg gtggagcagc gcattaaggc gcgcgttttg ctggcgaaaa
    34321 cgatagtggc tccagaaagc ctggatacgc agcaaattca tggtcagtat ctgcccaatc
    34381 accccttcat gtgctgcacg aatggcatcg gttgtttcag gctgccagag taaactggca
    34441 tgttccagta atcgggcgtg catgtttttc aatgcggtta aaagggctgt agcatcggaa
    34501 ctgctaggca ggatcatcat catcatgccg ccgcacaaaa taccgacaat cacctcgcaa
    34561 acgcgcgcct gagcgatatc ccacagctgg ctggcctcag taatattaac catcggaaag
    34621 gcgatgatgg cagccgtgta gcccgccagt tgaaatgcat acgcgacgtt attcgtgaag
    34681 tgcgcacagg cccaggtaca aaagccaagc cacgccgaca tgctcaatag aaaaaaccac
    34741 ggctcattga gcgtatgccc ggcaagaagc agtgccgcaa tggctcccag taaactgcca
    34801 gcgatgcgtc cgaggctttt gctgatgaca ccgccaacgg tgggaaagct aaccactgca
    34861 gccgaggtca tcgcccaata gggttcatcc agattcaaat aataggcaac cgtcagcgcc
    34921 agacacatgg caatggtatt gcgtaacgca taacgccatt gcgccagcgt ggccctgaac
    34981 cagggcaaat tgcgcaagga ccatgacgat gcgttcatta ttgacctacg gcaatgctac
    35041 aggtggtgcc cgacaccagc gtgacatcgc ctggaacttt atccagcgca aagcggacgg
    35101 gaacgcgttg ggcgagacga acccagggga cgttaggttt cacatccgga ataaggcttg
    35161 agtcactttc aacgctctga tcataaatcg cccgaccgat actggaaacg tgaccctgta
    35221 acgttttgtt atcactatag agggtaattt gtgctggtgc accttcgcgg atatggcgca
    35281 acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg gctgtcgaca agggcaaaca
    35341 gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt gagattagtc acccatccac
    35401 tcaccggggc gcgaatttcc gtttgcgcca gttgccattg tgtctgtttc agcgtcgcct
    35461 gtgcggcatc aacactggcc tgcatcgctt taacattgag gttagcggta tcgagttctt
    35521 cggcagaaat aaaattttgt gagagatgac gacgacgatt cgcttcgtta ttggcttttg
    35581 cgaggtcaga ttgcgctttc gccagctgag cctgtgcatt cagctcggcg atctgaaagg
    35641 gcgttttatc gatcgtgagc agaagatccc ccgcgttaac cagctggttg tcttttatat
    35701 tcagctcaac aatacgtccc gacacctgtg gggtgataga aacctgttct gcgcgtattt
    35761 tcccgtcgcg tgtccacgga gattgcatgt aataattcca cagccaccat ccggcaagaa
    35821 ccgcaactac cgctacaatg attgttgaga aatacttaat tgttttaatt gacatagtta
    35881 ccacgcaatc agtattgcca gagcaaggca aacgcaaatc gcaaacagcg ataaatccat
    35941 taacaaggga tgccagatgt caccggcgta gatccagcca cgaagcaggc ggtgtacgac
    36001 aagccagatg acgaatccaa acgcgaaggc tttgaaaaac ggaggaaagt agacggacgc
    36061 accgaacacc agatcttgta agggcaatcc tgttgcattg agcataaact tcacaaagta
    36121 gattccttta cgaccgaata tattgcgtta gattaataaa tattctttaa ctgcgaaaaa
    36181 tttacgcgca atttctgaaa aatatgtctg acggtaacca aatgcagcaa tacatttgtt
    36241 ttagcaatac aattgctgca cactattcta aaagccgcat aatatcttag caagctaatt
    36301 ataaggagat gaaattggaa tcgccactag gttctgatct ggcacggttg gtgcgcatat
    36361 ggcgtgctct gatagaccat cgcctgaaac cgctggagtt aacacaaacc cattgggtta
    36421 cgttacacaa tatccatcag ttacctccag accagtcgca aattcaactg gcaaaagcga
    36481 ttggcatcga gcagccatca ctggtccgta ctctggacca actggaagaa aaagggttaa
    36541 tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg tattaaactg acggaaaagg
    36601 cagagccgct gatcagcgaa atggaagctg ttattaacaa aacccgcgcg gaaattttac
    36661 atggcatctc cgcagaggaa ctggaacaac tgattacgct catcgcaaaa cttgagcata
    36721 atatcattga gttacaggcc aaagggtgaa atgaaggggg cgtgtggtca gtaacctgac
    36781 cacacgcaaa cttatttagc gcggagaaac ggtcacctga ctgccattgc tggccagtac
    36841 gacacgttgg cccggagaga aacgagtgtt gccttgtttc tgtaccacca tgatggtatt
    36901 accatcgtct ttacgaattt ccagctcgac accttgcgtt ttgttcattg cactctgtac
    36961 gccctgacca gctacgccac ctgcaacagc gcctgctgca gtagccagag aacgcccggt
    37021 tccgccacca acagtatttc ccaggaaacc accaagaaca gcaccgccaa ttgcaccgat
    37081 aacgttggaa tcatcaccgc cctgaatctg taccggacgt acgttaacga tggttccata
    37141 gctgacattc tgtacttgtt tcgcttcaga agcggtataa acatcccctg acagggtgtc
    37201 gttattaaca caaccgacaa gagacagacc taccattgaa acaaccaata cgcgtttaat
    37261 cattgaaaaa tctcctgttc accatgaaac gccacgcaag catccctcat ggtcaaagta
    37321 tatggcatat gtgaatccat ggttacatgt tctaccaaat atgagcaaat cataatgaaa
    37381 gtaggatcaa ccaattataa acaaaacatt catgatgatt attgatttga agataatgtt
    37441 ttactaccaa cggtaaaaat cggtcattcc gcatggtatt cacgaaaagt ttatgtttga
    37501 gtgtgcgcaa acgtcagatt gaaaaaggat gaactatgaa atcgggccgc tttattggcg
    37561 ttatgtcagg caccagcctt gatggtgttg atgttgtgtt ggcgacaatt gatgaacacc
    37621 gggtcgcaca gctggcaagt ttgagctggc cgatcccggt gtctctgaaa caggctgtac
    37681 tggatatttg ccagggccag cagcttacac tttcgcagtt tggacagctt gatactcaac
    37741 tcgggcgact ttttgctgat gcggtcaagg ccttgcttaa ggaacaaaac ctgcaggcga
    37801 gagatatagt tgcgatcggc tgtcacggtc aaaccgtctg gcatgaaccg acgggcgtgg
    37861 caccacacac tttacagatt ggcgataaca atcaaattgt ggcacgcacc ggaattacgg
    37921 ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg acaaggcgca ccgctggtac
    37981 ctgcgttcca tcatgccttg ctggctcacc caaccgagcg acgaatggtg ctcaatattg
    38041 gcggcatcgc caatctgtca ctgctcattc ctgggcagcc ggttggcggc tacgataccg
    38101 gtcctggtaa catgctgatg gatgcctgga tctggcgtca ggccggtaaa ccttacgata
    38161 aagatgccga gtgggcacgg gcgggtaaag ttattctccc actgctgcaa aatatgctca
    38221 gcgacccata tttctcgcaa cctgcaccga aaagcaccgg acgcgaatac tttaactacg
    38281 gctggctgga gcgccattta cgccattttc cgggtgttga tccccgtgat gttcaggcca
    38341 cactggcaga actcaccgcc gtgaccattt ctgaacaagt tttgttgagc ggtggctgcg
    38401 aacgattgat ggtatgtggt ggaggtagtc gtaatccgct actcatggcg cgtctggcgg
    38461 cattactgcc aggcacagaa gtcaccacca ccgatgccgt tggcattagt ggcgatgaca
    38521 tggaagcatt ggctttcgct tggcttgcct ggcggacgct ggcgggatta ccaggaaatc
    38581 tgccttccgt cactggcgca agccaggaga cggttctggg ggctattttc cccgccaact
    38641 cgtgatatca gaattaactg aattttttct tatcgtgtct gccgttacac ttggaagatt
    38701 aaggaggata tttgccctcc ggaccaggaa agtcttcggg atatgactat gaaaaaactg
    38761 ttaattatca ttttgccggt ccttctctcc ggctgtagcg cctttaatca actggttgag
    38821 cgaatgcaaa ccgatacgct ggaataccag tgtgatgaaa aaccgttgac ggtcaaactg
    38881 aataatccgc gccaggaagt cagttttgtt tacgataatc aactactgca tctcaaacag
    38941 ggcctttcag cctctggcgc gcgttactcc gacgggatct atgttttctg gtcgaaaggt
    39001 gaggaagcga ctgtctataa acgcgaccgc atcgtcttga ataactgtca gttacaaaat
    39061 ccacagcgtt gagatttttc caggggcggc gcacaatagc gtcacccaca gacaatccgt
    39121 aaagaaaacc atgtctgata acgacgaatt gcagcaaatc gcgcatctgc gccgtgaata
    39181 caccaaaggc gggttacgcc gccgcgatct tcccgccgat ccattaaccc tttttgaacg
    39241 ttggctctct caggcttgtg aagccaaact ggcggaccct accgcgatgg tggtcgctac
    39301 cgtggatgaa catggtcagc cttatcagcg catcgtttta ctcaaacatt acgacgaaaa
    39361 aggcatggtg ttttacacca acctcggtag ccgtaaagca catcaaattg aaaataatcc
    39421 gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc caggtgatgg tgatcggtaa
    39481 agcagaacga ctttcgactc tcgaagtgat gaaatatttt catagccgcc cgcgtgatag
    39541 ccagattggt tcatgggttt cgaagcagtc cagtcgcatt tctgcccgcg gtatccttga
    39601 aagtaaattc ctggagctga agcagaagtt tcaacaaggc gaagtgccat tgccgagctt
    39661 ttggggcggt tttcgcgtca gccttgaaca gattgagttc tggcagggcg gtgagcatcg
    39721 cctgcatgac cgctttttgt accagcgtga aaatgatgcg tggaagattg atcgtcttgc
    39781 accctgaaaa gatgcaaaaa tcttgcttta atcgctggta ctcctgattc tggcacttta
    39841 ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg gcaaaggtgc agtcgttata
    39901 tacatggaga ttttgatggc aagcagtaac ttgattaaac aattgcaaga gcgggggctg
    39961 gtagcccagg tgacggacga ggaagcgtta gcagagcgac tggcgcaagg cccgatcgcg
    40021 ctctattgcg gcttcgatcc taccgctgac agcttgcatt tggggcatct tgttccattg
    40081 ttatgcctga aacgcttcca gcaggcgggc cacaagccgg ttgcgctggt aggcggcgcg
    40141 acgggtctga ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa caccgaagaa
    40201 actgttcagg agtgggtgga caaaatccgc aagcaggttg ccccgttcct cgatttcgac
    40261 tgtggagaaa actctgctat cgcggcgaac aactatgact ggttcggcaa tatgaatgtg
    40321 ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta accagatgat caacaaagaa
    40381 gcggttaagc agcgtctcaa ccgtgaagat caggggattt cgttcactga gttttcctac
    40441 aacctgttgc agggttatga cttcgcctgt ctgaacaaac agtacggtgt ggtgctgcaa
    40501 attggtggtt ctgatcagtg gggtaacatc acttctggta tcgacctgac ccgtcgtctg
    40561 catcagaatc aggtgtttgg cctgaccgtt ccgctgatca ccaaagcaga tggcaccaaa
    40621 tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag cccgtacaaa
    40681 ttctaccagt tctggatcaa cactgcggat gccgacgttt accgcttcct gaagttcttc
    40741 acctttatga gcattgaaga gatcaacgcc ctggaagaag aagataaaaa cagcggtaaa
    40801 gcaccgcgcg cccagtatgt actggcggag caggtgactc gtctggttca cggtgaagaa
    40861 ggtttacagg cggcaaaacg tattaccgaa tgcctgttca gcggttcttt gagtgcgctg
    40921 agtgaagcgg acttcgaaca gctggcgcag gacggcgtac cgatggttga gatggaaaag
    40981 ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc aaccttcccg tggtcaggcg
    41041 cgtaaaacta tcgcctccaa tgccatcacc attaacggtg aaaaacagtc cgatcctgaa
    41101 tacttcttta aagaagaaga tcgtctgttt ggtcgtttta ccttactgcg tcgcggcaaa
    41161 aagaattact gtctgatttg ctggaaataa tgcattaagt ggaaaggggg agtgagaaat
    41221 cactccccct ggtttttata cagggaacat gatgaaaaat attctcgcta tccagtctca
    41281 cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt ccgatgcgcc gcctgggcgc
    41341 gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac acccaatacg gcaaatggac
    41401 tggctgcgtg atgccgccca gccatttaac cgaaattgtg caaggcattg ccgccattga
    41461 taaattacac acctgtgatg ccgtattaag tggctatctg ggatcggcgg agcagggtga
    41521 acatatcctc ggtatcgtcc gtcaggtgaa agcagcgaat ccgcaggcga aatatttttg
    41581 cgatccggta atgggtcatc cagaaaaagg ctgtatcgtt gcaccgggtg tcgcagagtt
    41641 tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg ccgaatcttg ttgagctgga
    41701 aatactctgt gagcatccgg taaataacgt cgaagaagcg gttctggcag cgcgcgaact
    41761 cattgcgcaa gggccacaaa ttgtattggt taaacacctg gcgcgagctg gctacagccg
    41821 tgaccgtttt gaaatgttgc tggtcaccgc cgatgaagcc tggcatatca gccgtccact
    41881 ggtggatttt ggtatgcgcc agccggtagg tgttggtgat gtgacgagcg gtttactgct
    41941 ggtgaaactg cttcaggggg caacgctgca ggaggccctg gaacatgtga ccgctgcagt
    42001 ctacgaaatc atggtgacca ccaaagcaat gcaggaatat gagttgcaag tggttgctgc
    42061 tcaggatcgt attgccaaac cagaacatta cttcagcgca acaaagctct gaaatatctc
    42121 atttcggccc acaacgaact gtgggccgac ggctattact ttaagccttc cgctgacagc
    42181 gcgtcttgta cttccggacg ttcagccata cgttgcataa atgccgcaat gtgctctaac
    42241 ccttccagat tcagtttcac cgcgtatgcc cagcgcagta ccgtaaacag ataggcatca
    42301 gcaattgtaa atctttgccc gcagatccag tgctcatcct ccagtgcctc gttcacatat
    42361 tgcagtttct tctccagctg cgcgcgaact gtcggtttgt actcttccgg tgtatcaggg
    42421 cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga tgtaattcag ccattcgatg
    42481 gttttatagc gggaaatact gtttaccggt gccagcaact ggcggtcggg gacgctgtcg
    42541 gcaagatact gcataatcgc tacaccttcc gtcagcaaag taccgtcatc cagcagcaat
    42601 gcaggtacct gtcccttagg gttaacggca aagtaatcgt caccgttttc gagacgtttt
    42661 ttcattaaat ccacactgac gagcgtaaaa tcctttccgc tctcacgcag ggtgatatgg
    42721 gaagcgagag agcaggcgcc cggtttgtag aacaatttca tcggtaactc cttttgtctg
    42781 tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa gccgctaacg aaaagttagc
    42841 ggcttgtaaa agagtttccc tgaattttac gctacggctg ctttcgccgc tttgtctgca
    42901 gcgtcatcct gcgtcatgcg gtgcagtttc ggcgcggtca gcagcatcag tactgcaata
    42961 acggcagtag cgacaccaat ctgcaagaat acgcgaccat agacttccag tgacatcagc
    43021 ggatcggtaa cgttatccgg cacagccatc atacccgcaa cataaccacc aatcaggttt
    43081 gcaccggcag tggtcaggaa ccagctaccc ataatgaagc ccatcagacg ctgcggaacg
    43141 agttgtgcaa ccattgccag acccagacca gagatcatca gttccccgat actctgcagg
    43201 ccatagctcg cgaccagcca gcttacagac acgatacctg cgtcagacgc gaatttcgca
    43261 cccagcggca gaatcaggaa cgcaccagaa cacatcacca taccgattgc aaacttggtt
    43321 ggcatcggca gggtatcgcc catcttgtta tagatagcgg ccagaatcgg gctaccaatg
    43381 atgatccaga acgggttcag tgcctgatac tgctcaggtt ctacggccag gcccagaatg
    43441 gtatgttcaa cgttacgaat cgcaaagaag ttcagtgacg ttggcatctg gctgtacagc
    43501 acgaagaaga taatggcttc gagcatcagg atgaaggcaa cgatcatttt acgacgcgca
    43561 gcacctttca tcgcgaaggc ttctttaccg aagataacca cgataccgaa ggcaacaacg
    43621 cccagcgcca tacgcgcaac ttcctgattg tgcagcagcc aggtggcgat agcgatcagt
    43681 gccacaacac caataatggt cagcagcagg ttacggtagt tgataggctc gaagtctggt
    43741 tttgaaccgt actgtttaac ccagcgttgg cagaaggcga agttaacgat agtgatcagc
    43801 aggcctacaa cgctcaacgc aaacgcaaca ctccagccgt atttcgcggc cagccacggg
    43861 gtggcgatca tagagaagaa agagccgatg ttgacggaca tgtagtacat ggtgaatgca
    43921 ccgtccagac gcgggtcgtt tttctcatag catgtagaaa gcagagaaga cgggttagct
    43981 ttaaacaggc cgttaccgac cgcaatagcc gccataccca tataaacgat accggcgtcg
    44041 tgaccagacc aggcaaccag agcataacca atcgccagca caatagcgcc gagcataatt
    44101 acgcgtttag tacccagtac cttgtcacct aaccagccgc cgatagcgac cagaccataa
    44161 accagggcac taaaggaaga gaaaagggtg attgaatccg cttcagacat acccagttgt
    44221 ttaaccaggt aaacagccat aattccttgt aggccgtaat aaccaaaacg ttcccataac
    44281 tcaatcgaga agatgagata gaacgccttc ggttgtttga aagcgttcaa actgacgctt
    44341 tcagttggtt tttggtttgc agtggacact tttacctctt tttttacatc ccatattaac
    44401 gggggtgctt tctcgcgtta tgtctaaaaa acatacgcgc aattgttata gtgggagggg
    44461 aaacggcagg taatgttcac tatcttgcac tttcagacaa gtcctttgta ataatctgtt
    44521 acatataact gaggcgggga attcaaccat tccgggaaat gttatccagc gttaaatttt
    44581 tcaaaaaacg taacggcaga ttttttcact gcattgagag ggggatgatg gcttgacggc
    44641 gatatgttct gctatttagc gtttttccta gtgagatagt ccagtttctt aaaaatagcc
    44701 agtgtaatgt tttgcaggtc aatatttgtt gcgtgtgtaa atttaaatga ttttaacctg
    44761 attcttgcaa tgtttttaca ttgccacagc atcgtgatct tgatcactaa tgaatagaaa
    44821 atttcgctat taaaaaaaca attaacctgc atttttggtt atccgcaaac ggattacctg
    44881 atgataacgc taagcaatgg catcattgac gttaaaaaga tgttttaaaa gaggataaag
    44941 aaaggttatc aatggggtaa tcggtgttac cccttttctt cagatgtcaa ctttctcttt
    45001 gtattcacaa agatcttcaa taatacaaga gccacagcgg ggcttgcggg caatgcaggt
    45061 ataacgcccg tgcaggatca accaatggtg gcagtcgact ttaaactctg ccggaaccac
    45121 tttcagtagc ttttcttcta cctgttcgac gtttttcccc ggcgcaaatt gagtacgatt
    45181 acaaacgcgg aaaatgtgcg tgtcgacagc aatagtcggc cagccgaatg cagtgtttaa
    45241 tacgacgttg gctgttttac gacctacgcc gggcagggct tcaagcgcag cacgatcttc
    45301 cggaacctcg ctattatgct gctccagtaa gatacggcag gttttgatga tattttctgc
    45361 tttgctgtta taaagcccaa tcgttttgat ataggttttc accccttcaa cgcccagttc
    45421 aagcatcgct gcaggcgtat tcgccaccgg gtagagtttc gccgtcgcct tattaacact
    45481 gacatcggtt gcctgagcgg aaagcagtac ggcaatcagc aattcaaaag gcgaactgaa
    45541 attaagctca gtggtgggat gaggattgtt ctcacgcagg cgagtgagga tctccaggcg
    45601 ttttgctttg ttcatcagac attccctgtt tcaccgtttg gcaatgcacg ttcggcagct
    45661 gcttcagtac ggcgcttttt cattctttca tcaatcaggt attttcctgc cagcatcagt
    45721 cccaggccaa taaatgcacc tggtggcagc atcgccagca ggaaagggga gtcggtgtgg
    45781 aaaatctcca cgcgtaatac ttttgcccag ctacctaaca gcgcatctgc accgtcaaac
    45841 aatgtgccat tgccgataat ttcgcgtagt gaacccagca cgaacatggc gcaggttgcg
    45901 cccataccaa ttgaaaagcc gtccagtgcc gaaagcgccg gacctttttt ggcagcgaag
    45961 gcttcagcgc ggcccacaac gatacagtta gtgacaatca gcggaataaa aatccctaat
    46021 gattgataca ggccaaaggc gtaggcgttg atcagcatct gtacagcgct gaccaccgag
    46081 gcgatgatca tcacgtaaat gggaatgcgg atctcggctg gcgtccagtg acgcagcgtc
    46141 gaaatggtca ggttggtcag cgtcagtacc agcgtagtcg caagtcctaa acccagagcg
    46201 ttagtggcag tagacgtgac agccaacaga ggacaaaggc cgagcaactg gaccagcgca
    46261 gagttgtttt tccacaaccc ctgaacaata acgtctttaa tttcgctcac gctttattct
    46321 ccacaggcag gaagttgaga aagttgttcc ggtaacgtct gagcgtacaa tccggcgcgt
    46381 tttaccgcat taaccaccgc gcggggagta atcgtcgcgc cggtgaactg gtcgaaatta
    46441 ccaccatctt tcttcaccgc ccagtgcgca tcatccgcac cactgatttt tttacccgca
    46501 aaatgggtga tccagtcaga aaggcgcagt tcgattttat cgccaagccc tggcgtttcg
    46561 tggtgctctg tcacgcgcgt gccaagtacc gtgccgttaa aatcggctcc caccagcagc
    46621 tgaatcgcac cggaatagcc atctggcgcg gttgcttcca gaacggcggc taccggtttg
    46681 tcatcctgtt tggcgatgta aacccgatgc tcacctttac ctaactctgg cgcagttacc
    46741 agatagcaac tctgtgccag cgcattgtta tagcgttcgg ctggcagcac ctgatcaaat
    46801 aaagcctttt gttgcagact ggcctgttca gcaatcgtcg ttttggtcat ctggttgatg
    46861 gccgcagtta accctgttga acccgctgca aatagcgcca acgtaatgcc gtgttttcgg
    46921 atagttttca gcatggttta ccctttgcga tggccgtaga cgcgcggacg cgtgtagtaa
    46981 tcgatcagag gaaccgtgat gttcgccagc aggacggcaa aagccacgcc gtcaggatag
    47041 ccgccgaaac tgcggatcaa ccagactaat aagcccgcaa gcgcgccgaa aataagacga
    47101 ccacgattgg tcgtagaagc ggtaaccggg tcagtcaaaa taaagaatgc gccgagcatg
    47161 gtcgctccag acagcagatg aatttgcggt gctgccagtg tttctggtga gaacaaccag
    47221 cccaacgttg cgcataacgc cagcgttact aagaagctga ggggaatatg ccagcgaatc
    47281 gctttctgcc atagcaacca tacgccgcca gccagccagg cgagatttac ccattgccaa
    47341 ccagcgcccg ccagaatacc gctgtagatc ggatattgca taatctgttc aaccgaatga
    47401 ccggcacgga cagaggtttt aaatgtatcc agcggtgtcg cctgactaat gccatcaata
    47461 cctaagcgta gtgtgttcat atcaccacca ctggcggtat gcccgctgaa aataacctgt
    47521 atggcgtcga taaaaccagg gatgttgacc gcaatttcat gtggcggtaa ccagctggtc
    47581 atctgcaccg ggaaggagat cagtaagacc acataaccaa tcattgccgg attaaacggg
    47641 ttttgcccca gaccgccata caactgttta gcgatgataa ccgcaaacac cgtacccagc
    47701 acgaccatcc accatggcgc gaggggggga atacttaccg ccagcaataa gcctgtcagc
    47761 aatgctgagt tatctttcaa cgtggcggct accgactgct tgcgtagttt gagtacgaga
    47821 gcttcggcta acagagcact aaccgatgcc aacaggatct gaacgagagt accccaacca
    47881 aaaaaccaca gttgcgctgc gattcctggc acggctgcga gcaacaccaa cagcataatg
    47941 cgcgatgtct ggcgctggtt atgggtataa ggggagctag ctattctgaa taccatttag
    48001 tcctcgttta caaccttttg ctggacggct tttttggcct gaacgcgggc aatagccgcg
    48061 gcaactgccg ctttgcgcgg atcaacctgt tcttctggtt ctgcattagc ctgttgctgt
    48121 tccagcttgc gtgctttggc gcgtgcaata gccgctgcga cggcagcttt gcgcggatca
    48181 acctgttctt ctggctccgc attagcctgt tgctgttcca gtttgcgcgc tttggcacgg
    48241 gtaatagcgg cttcgacgg
//
LOCUS       DAZFDC010000018        48121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFDC010000018 DAZFDC010000000
VERSION     DAZFDC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.018"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            271..1587
                     /gene="fucP"
                     /locus_tag="ACHJ48_001201"
     CDS             271..1587
                     /gene="fucP"
                     /locus_tag="ACHJ48_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO4706802.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGSHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1620..3395
                     /gene="fucI"
                     /locus_tag="ACHJ48_001202"
     CDS             1620..3395
                     /gene="fucI"
                     /locus_tag="ACHJ48_001202"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO4706803.1"
                     /translation="MNKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3504..4922
                     /gene="fucK"
                     /locus_tag="ACHJ48_001203"
     CDS             3504..4922
                     /gene="fucK"
                     /locus_tag="ACHJ48_001203"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO4706804.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPLKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQFRYFYPQTEPEFIEEV"
     gene            4924..5346
                     /gene="fucU"
                     /locus_tag="ACHJ48_001204"
     CDS             4924..5346
                     /gene="fucU"
                     /locus_tag="ACHJ48_001204"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO4706805.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5404..6135
                     /gene="fucR"
                     /locus_tag="ACHJ48_001205"
     CDS             5404..6135
                     /gene="fucR"
                     /locus_tag="ACHJ48_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO4706806.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTG"
     gene            complement(6179..7279)
                     /gene="rlmM"
                     /locus_tag="ACHJ48_001206"
     CDS             complement(6179..7279)
                     /gene="rlmM"
                     /locus_tag="ACHJ48_001206"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO4706807.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7272..7667)
                     /gene="ygdD"
                     /locus_tag="ACHJ48_001207"
     CDS             complement(7272..7667)
                     /gene="ygdD"
                     /locus_tag="ACHJ48_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO4706808.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7686..8603)
                     /gene="gcvA"
                     /locus_tag="ACHJ48_001208"
     CDS             complement(7686..8603)
                     /gene="gcvA"
                     /locus_tag="ACHJ48_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO4706809.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(8954..9181)
                     /gene="ygdI"
                     /locus_tag="ACHJ48_001209"
     CDS             complement(8954..9181)
                     /gene="ygdI"
                     /locus_tag="ACHJ48_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO4706810.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9373..10578
                     /gene="csdA"
                     /locus_tag="ACHJ48_001210"
     CDS             9373..10578
                     /gene="csdA"
                     /locus_tag="ACHJ48_001210"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO4706811.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVIVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10578..11021
                     /gene="csdE"
                     /locus_tag="ACHJ48_001211"
     CDS             10578..11021
                     /gene="csdE"
                     /locus_tag="ACHJ48_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO4706812.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11072..11878)
                     /gene="tcdA"
                     /locus_tag="ACHJ48_001212"
     CDS             complement(11072..11878)
                     /gene="tcdA"
                     /locus_tag="ACHJ48_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO4706813.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINSECCV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12117..13214)
                     /gene="mltA"
                     /locus_tag="ACHJ48_001213"
     CDS             complement(12117..13214)
                     /gene="mltA"
                     /locus_tag="ACHJ48_001213"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO4706814.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13423..13499
                     /locus_tag="ACHJ48_001214"
     tRNA            13423..13499
                     /locus_tag="ACHJ48_001214"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13457..13459,aa:Met,seq:cat)
     gene            complement(13573..14826)
                     /gene="amiC"
                     /locus_tag="ACHJ48_001215"
     CDS             complement(13573..14826)
                     /gene="amiC"
                     /locus_tag="ACHJ48_001215"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO4706815.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15058..16389
                     /gene="argA"
                     /locus_tag="ACHJ48_001216"
     CDS             15058..16389
                     /gene="argA"
                     /locus_tag="ACHJ48_001216"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO4706816.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16451..18277)
                     /gene="recD"
                     /locus_tag="ACHJ48_001217"
     CDS             complement(16451..18277)
                     /gene="recD"
                     /locus_tag="ACHJ48_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO4706817.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEESHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPTGDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18277..21819)
                     /gene="recB"
                     /locus_tag="ACHJ48_001218"
     CDS             complement(18277..21819)
                     /gene="recB"
                     /locus_tag="ACHJ48_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO4706818.1"
                     /translation="MSDVAETLDPLRLPFQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAKWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     RNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVSAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMMRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLNFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQARGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(21812..24700)
                     /gene="ptrA"
                     /locus_tag="ACHJ48_001219"
     CDS             complement(21812..24700)
                     /gene="ptrA"
                     /locus_tag="ACHJ48_001219"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO4706819.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24876..28244)
                     /gene="recC"
                     /locus_tag="ACHJ48_001220"
     CDS             complement(24876..28244)
                     /gene="recC"
                     /locus_tag="ACHJ48_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO4706820.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDHELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNALFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVSGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(28257..28580)
                     /gene="ppdC"
                     /locus_tag="ACHJ48_001221"
     CDS             complement(28257..28580)
                     /gene="ppdC"
                     /locus_tag="ACHJ48_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO4706821.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLTSR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGRAGEMTRLH
                     CPNRQ"
     gene            complement(28565..28972)
                     /gene="ygdB"
                     /locus_tag="ACHJ48_001222"
     CDS             complement(28565..28972)
                     /gene="ygdB"
                     /locus_tag="ACHJ48_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO4706822.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCLLYAATGARVCLRLLEDNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(28969..29532)
                     /gene="ppdB"
                     /locus_tag="ACHJ48_001223"
     CDS             complement(28969..29532)
                     /gene="ppdB"
                     /locus_tag="ACHJ48_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4706823.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEQVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(29523..29993)
                     /gene="ppdA"
                     /locus_tag="ACHJ48_001224"
     CDS             complement(29523..29993)
                     /gene="ppdA"
                     /locus_tag="ACHJ48_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO4706824.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISFIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIGFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30177..30971)
                     /gene="thyA"
                     /locus_tag="ACHJ48_001225"
     CDS             complement(30177..30971)
                     /gene="thyA"
                     /locus_tag="ACHJ48_001225"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO4706825.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(30978..31853)
                     /gene="lgt"
                     /locus_tag="ACHJ48_001226"
     CDS             complement(30978..31853)
                     /gene="lgt"
                     /locus_tag="ACHJ48_001226"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO4706826.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(32004..34250)
                     /gene="ptsP"
                     /locus_tag="ACHJ48_001227"
     CDS             complement(32004..34250)
                     /gene="ptsP"
                     /locus_tag="ACHJ48_001227"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4706827.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34263..34793)
                     /gene="rppH"
                     /locus_tag="ACHJ48_001228"
     CDS             complement(34263..34793)
                     /gene="rppH"
                     /locus_tag="ACHJ48_001228"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO4706828.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35146..35292)
                     /gene="ygdT"
                     /locus_tag="ACHJ48_001229"
     CDS             complement(35146..35292)
                     /gene="ygdT"
                     /locus_tag="ACHJ48_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO4706829.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            35478..36167
                     /gene="mutH"
                     /locus_tag="ACHJ48_001230"
     CDS             35478..36167
                     /gene="mutH"
                     /locus_tag="ACHJ48_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO4706830.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36236..36949
                     /gene="ygdQ"
                     /locus_tag="ACHJ48_001231"
     CDS             36236..36949
                     /gene="ygdQ"
                     /locus_tag="ACHJ48_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4706831.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37087..37305
                     /gene="ygdR"
                     /locus_tag="ACHJ48_001232"
     CDS             37087..37305
                     /gene="ygdR"
                     /locus_tag="ACHJ48_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO4706832.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            37413..38453
                     /gene="tas"
                     /locus_tag="ACHJ48_001233"
     CDS             37413..38453
                     /gene="tas"
                     /locus_tag="ACHJ48_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO4706833.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(38485..39678)
                     /gene="lplT"
                     /locus_tag="ACHJ48_001234"
     CDS             complement(38485..39678)
                     /gene="lplT"
                     /locus_tag="ACHJ48_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO4706834.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHEQLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(39671..41830)
                     /gene="aas"
                     /locus_tag="ACHJ48_001235"
     CDS             complement(39671..41830)
                     /gene="aas"
                     /locus_tag="ACHJ48_001235"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO4706835.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEVEQHDE"
     gene            42416..43447
                     /gene="galR"
                     /locus_tag="ACHJ48_001236"
     CDS             42416..43447
                     /gene="galR"
                     /locus_tag="ACHJ48_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO4706836.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLLKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(43454..44716)
                     /gene="lysA"
                     /locus_tag="ACHJ48_001237"
     CDS             complement(43454..44716)
                     /gene="lysA"
                     /locus_tag="ACHJ48_001237"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO4706837.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMFDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVEPRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            44838..45773
                     /gene="lysR"
                     /locus_tag="ACHJ48_001238"
     CDS             44838..45773
                     /gene="lysR"
                     /locus_tag="ACHJ48_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO4706838.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLVRYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDELCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(45760..46452)
                     /gene="ygeA"
                     /locus_tag="ACHJ48_001239"
     CDS             complement(45760..46452)
                     /gene="ygeA"
                     /locus_tag="ACHJ48_001239"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO4706839.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(46581..47999)
                     /gene="araE"
                     /locus_tag="ACHJ48_001240"
     CDS             complement(46581..47999)
                     /gene="araE"
                     /locus_tag="ACHJ48_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO4706840.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        11813 a        13072 c        11901 g        11335 t
ORIGIN      
        1 acggtcatct ggtttgtgtt ttttatgatt tattttctga aacgggcatg aaatttcgat
       61 tattaaagtg atggtagtca cataaagtca ccttccagct aataagtgtg accgccgtca
      121 tattacagag cgttttttat ttgaaaatga atccatgagt tcatttcaga caggcaaata
      181 ttcactgata tgaagcccga actcgctagt tttgcacttt tgaaaacata accgattacg
      241 tgcttaagct tctgaaccta agaggatgct atgggaaaca catcaataca aacgcagagt
      301 taccgtgcgg tagataaaga tgcagggcaa agcagaagtt acattattcc attcgcgctg
      361 ctgtgctcac tgttttttct ttgggcggta gccaataacc ttaacgacat tttattacct
      421 caattccagc aggcttttac actgacaaat ttccaggctg gcctgatcca atcggccttt
      481 tactttggtt atttcattat cccaattcct gctgggatat tgatgaaaaa actcagttat
      541 aaagcaggaa ttattaccgg attattttta tatgccttgg gtgctgcatt attctggccc
      601 gccgcagaaa taatgaacta caccttattt ttagttggcc tatttattat tgcagccgga
      661 ttaggttgtc tggaaactgc cgcaaaccct tttgttacgg tattagggcc ggaaagcagt
      721 ggtcacttcc gcttaaatct tgcgcaaaca tttaactcat ttggcgcaat tatcgcggtt
      781 gtctttgggc aaagtcttat tttgtctaac gtgccacatc aatcgcaaga cgttctcgat
      841 aaaatgtctc cagagcaatt gagtgcgtat aaacacagcc tggtattatc ggtacagaca
      901 ccttatatga tcatcgtggc tatcgtgtta ctggtcgccc tgctgatcat gctgacgaaa
      961 ttcccggcat tgcagagtga taatcacagt gacgccaaac aaggatcgtt ctccgcatcg
     1021 ctttctcgcc tggcgcgtat tcgccactgg cgctgggcag tattagcgca attctgctac
     1081 gttggcgcac aaacggcctg ctggagctat ttgattcgct acgctgtaga agaaattcca
     1141 ggcatgactg caggctttgc cgctaactat ttaaccggaa ccatggtgtg cttctttatt
     1201 ggtcgtttca ccggtacctg gctcatcagt cgcttcgcac cacacaaagt cctggccgcc
     1261 tacgcattaa tcgctatggc actgtgcctg atctcagcct tcgctggcag tcatgtgggc
     1321 ttaatagccc tgactttatg cagcgccttt atgtcgattc agtacccaac aatcttctcg
     1381 ctgggcatta agaatctcgg ccaggacacc aaatacggtt cgtccttcat cgttatgacc
     1441 atcattggcg gcggtattgt cactccggtc atgggttttg tcagtgacgc ggcgggcaac
     1501 atccccactg ctgaactgat ccccgcactc tgcttcgcgg tcatctttat ctttgcccgt
     1561 ttccgttctc aaacggcaac taactgaaca tattttccga ataaagtgag gaatctgtaa
     1621 tgaacaaaat cagcttaccg aaaattggta tccgcccggt tattgacggt cgtcgcatgg
     1681 gtgttcgtga gtcgcttgaa gaacaaacaa tgaatatggc gaaagctacg gccgcactgc
     1741 tgaccgagaa actgcgccat gcctgcggag ctgccgtcga gtgtgtcatt tccgatacct
     1801 gtatcgcggg tatggctgaa gccgctgctt gcgaagaaaa attcagcagc cagaatgtag
     1861 gcctcaccat tacggtaacg ccttgctggt gctatggcag tgaaaccatc gacatggatc
     1921 caacccgccc gaaagccatt tggggcttta acggcactga acgtcccggc gctgtttacc
     1981 tggcagcggc tctggcagct cacagccaga aaggcatccc ggcattctcc atttacggtc
     2041 atgacgttca ggatgccgat gacacatcga ttcctgccga tgttgaagaa aaactgctgc
     2101 gctttgcccg cgccggtttg gccgtcgcca gcatgaaagg taaaagctat ctgtcgctgg
     2161 gcggcgtttc gatgggtatc gccggttcta ttgttgatca caacttcttt gaatcctggc
     2221 tgggaatgaa agtccaggcg gtggatatga ccgaactgcg tcgccgtatc gatcagaaga
     2281 tttacgacga agccgaactg gaaatggcac tggcctgggc tgataaaaac ttccgctatg
     2341 gcgaagatga aaataacaaa cagtatcaac gcaatgccga gcaaagccgc gcagttctgc
     2401 gcgaaagttt actgatggcg atgtgtatcc gcgacatgat gcaaggcaac agcaaactgg
     2461 ccgatattgg tcgcgtggaa gaatcacttg gctacaacgc catcgctgcg ggcttccagg
     2521 ggcaacgtca ctggaccgat caatatccca atggtgacac cgccgaagcg atcctcaaca
     2581 gttcatttga ctggaatggc gtgcgcgaac cctttgtcgt ggcgaccgaa aacgacagtc
     2641 ttaacggcgt ggcaatgcta atgggtcacc agctcaccgg caccgctcag gtatttgccg
     2701 atgtgcgtac ctactggtca ccagaagcaa ttgagcgtgt aacggggcat aaactggatg
     2761 gactggcaga acacggcatc atccatttga tcaactccgg ttctgctgcg ctggacggtt
     2821 cctgtaaaca acgcgacagc gaaggtaacc cgacgatgaa gccgcactgg gaaatctctc
     2881 agcaagaggc tgacgcttgc ctcgccgcta ccgaatggtg cccggcgatc cacgaatact
     2941 tccgtggcgg cggttactct tcccgcttcc ttaccgaagg cggcgtcccg ttcaccatga
     3001 ctcgtgtcaa catcatcaaa ggcctgggac cggtactgca aatcgcggaa ggctggagcg
     3061 tggaattgcc gaaggatgtg catgacatcc tcaacaaacg caccaactca acctggccaa
     3121 ccacctggtt tgcaccgcgc ctcaccggta aagggccgtt tacggatgtg tactcggtaa
     3181 tggcgaactg gggcgctaac catggggttc tgaccatcgg ccacgttggc gcagacttta
     3241 tcactctcgc ctccatgctg cgtatcccgg tatgtatgca caacgttgaa gagaccaaag
     3301 tgtatcgtcc ttctgcctgg gctgcgcacg gcatggatat tgaaggccag gattaccgcg
     3361 cttgccagaa ctacggtccg ttgtacaagc gttaatacat tactccctga tgtgatgccc
     3421 ggtcgctccg gctgccgggc ctgaacaagc aagagtggtt agccggataa gcaatgttat
     3481 ccggctatat tgcaggagcg atcatgaaac aagaagtgat cctggtactc gactgtggcg
     3541 cgaccaatgt cagggccatc gcggttaatc ggcagggaaa aattgttgcc cgcgcctcaa
     3601 cgcctaatgc cagcgatatc gcgatggaaa acaacacctg gcaccagtgg tctttagacg
     3661 ccattttgca acgctttgct gattgctgtc ggcaaatcaa tagtgaactg actgaatgcc
     3721 acatccgcgg tatcgccgtc accacctttg gtgtggatgg cgctctggta gataagcaag
     3781 gcaatctgct ctatccgatc attagctgga aatgtccgcg aacagcagcg gttatggaca
     3841 atattgaacg gttaatctcc gcacagcggt tgcaggctat ttctggcgtc ggagccttta
     3901 gtttcaatac gttatataag ttggtgtggt tgaaagaaaa tcatccacaa ctgctggaac
     3961 gcgcgcacgc ctggctcttt atttcgtcgc tgattaacca ccgtttaacc ggcgaattca
     4021 ctactgatat cacgatggcc ggaaccagcc agatgctgga tatccagcaa cgcgatttca
     4081 gtccgcaaat tttacaagcc accggtattc cacgccgact cttccctcgt ctggtggaag
     4141 cgggtgaaca gattggtacg ctacagaaca gcgccgcagc aatgctcggc ttacccgttg
     4201 gcataccggt gatttccgca ggtcacgata cccagttcgc cctttttggc gctggtgctg
     4261 aacaaaatga acccgtgctc tcttccggta catgggaaat tttaatggtt cgcagcgccc
     4321 aggttgatac ttcgctgtta agtcagtacg ccggttccac ctgcgaactg gatagccagg
     4381 cagggttgta taacccaggt atgcaatggc tggcatccgg tgtgctggaa tgggtgagaa
     4441 aactgttctg gacggctgaa acaccctggc aaatgttgat tgaagaggct cgtctgatcg
     4501 cgcctggcgc ggatggcgtg aaaatgcagt gtgatttatt gtcgtgtcag aacgctggct
     4561 ggcaaggagt gacgcttaat accacgcggg ggcatttcta tcgcgcggcg ctggaagggt
     4621 taactgcgca attacagcgc aatctacaga tgctggaaaa aatcgggcac tttaaggcct
     4681 ctgaattatt gttagtcggt ggaggaagtc gcaacacatt gtggaatcag attaaagcca
     4741 atatgcttga tattccgtta aaagttctcg acgacgccga aacgaccgtc gcaggagctg
     4801 cgctgttcgg ttggtatggc gtaggggaat ttaacagccc ggaagaagcc cgtgcacaga
     4861 ttcattatca gttccgttat ttctacccgc aaactgaacc tgaatttata gaggaagtgt
     4921 gaaatgctga aaacaatttc gccgttaatt tctcccgaac tattgaaagt gctggcagag
     4981 atgggacatg gagatgaaat tattttttcc gatgctcact ttcccgccca ttcgatggga
     5041 ccgcaggtga tccgcgctga tggcctgttg gtgagcgact tgctccaggc gattatcccg
     5101 ttatttgagc tggacagtta tgcaccgccg ctggtgatga tggcagcggt agaaggtgac
     5161 actctcgatc ctgaagtaga acgacgttac cgtaatgcgc tttcactaca agccccgtgt
     5221 cctgacatca tccgcatcaa tcgttttgcg ttttatgaac gggcgcaaaa agcctttgcg
     5281 atcgttatca caggcgaacg agcgaagtac gggaatattc ttttaaaaaa aggggtaaca
     5341 ccgtaatctc ataccggtac gcccgcatga cgcgggcggt tatcgaatga tggggtgaaa
     5401 aatatgaaag cggcacgcca gcaagcgata gtcgacctgc tgctgaacca taccagcctg
     5461 accacggaag ctctctctga acagctaaag gtcagtaaag aaaccattcg tcgcgatctc
     5521 aatgaattac agacgcaggg taaaattctg cgcaatcatg gacgcgctaa atatatccac
     5581 cgtcaaaatc aagacagtgg cgatcccttt cacatcaggc tgaaaagcca ttatgcgcat
     5641 aaagcagata tcgcgcgcga ggcgctcgcg tggattgaag aagggatggt gatagcctta
     5701 gacgccagtt caacttgctg gtatctggca cgccagttgc ctgacatcaa cattcaggtc
     5761 ttcaccaata gccatccgat ttgccatgaa ctcggtaaac gcgaacgcat tcaactgatc
     5821 agttccggcg gcacacttga gcgcaaatat ggctgttacg tcaatccctc gctgatttcc
     5881 caacttaaat cgctggaaat cgatctgttt attttttctt gtgaagggat cgatagcagc
     5941 ggcgcactgt gggactccaa tgctatcaac gctgattaca aatcaatgct attaaaacgc
     6001 gccgcgcaat cgttgttatt gattgataaa agtaaattta atcgttcagg ggaagcccgc
     6061 atcgggcatc tggatgaggt aacgcacatt atttctgatg aacgccaggt tgcaacttct
     6121 ttggtaacag gctgacggaa agggtagcag gccggagacg acgccccggc cttgcctgtt
     6181 atcgctcgtc gcgacgacca cccaccgcag cccagatacg gcggacgtgc accgtcactt
     6241 cttcgcgatc gtgatacaac tgccgcgcct gaatctgagc atttatgcca tgttcatcaa
     6301 gctgtgcctg aatatacgcc agattgtgtg acacttcttc gtagcgtttt ttcatcggca
     6361 gtttgaggtt gaaaatggtt tcacgacacc agccattaac cagccactgc gccatcaatg
     6421 ccgcaacttt cgccggtttt tcaaccatat cgcataccat ccaggagata ttgctgcgcg
     6481 tcggacggaa tttgaaaccg tcttcccgca gccacgtcac ctgtccggta tccatcagac
     6541 tttgcgccat cgggccgttg tcgacggaat aaacccacat gttgcgcttc accagttggt
     6601 aggtccagcc gccagggcaa gcgcctaaat ccaccgccca catcccgttc gccaggcgtt
     6661 catcccactc atccgcagga ataaacacat gaaatgcctc ttccagtttg agcgtggaac
     6721 gactcggcgc atctgccgga aatttcaggc gcggaatgcc catatagaac ggcgaattat
     6781 tgttgctgta tgagtaaccg gtatagcagc agcctggtgc aataaagaat acatgcacaa
     6841 ccggacgctt cggcgtttca tagttcgcca gcaccccggc atcgcgcaag gcagcgcgta
     6901 gcggcacggt gaatttacgg cagaatttca gcaactcttt gctttcgttg gtatcggcaa
     6961 cttcaacacg cagttcaccg cccttctcta ctacgccctg caacatgccg acaatggggg
     7021 taatacgatc ttctggcggc aaatgctgca agagttcccc caccacaaac cactggcggg
     7081 caaaaattaa tgaactgaac ggcagctcac ggattaactt atcgccatca tcaggttgat
     7141 aacattcata aatgacataa cccgcattct ctttcacgcg ggcaaaaccg aaaatctccc
     7201 gctggccggc tttatcggta atttctgcgg cgcactcttt ttcaaagccc ggacggcaca
     7261 gcaatacaac cttattcatg acttacgccc ttgcgcttta aacggatagc accaactaac
     7321 attaacgccc agcccgcaag gaagctcacg ccgccaaccg gagtgacaaa cgcccacaaa
     7381 cgcagatggg acagtgccag gcaataaagg ctgccgctga acagcaccgt gcctaacgcg
     7441 aggaaaacgc tactccagta aaaccagata ctgatgcgac gctgcattgc caccgccaga
     7501 cctaagatcg ccagcgtatg aaacgcctgg tattcgaggc cggtctggat ccagcccatc
     7561 tcaacggccc ccatggtttt acttaacaca tgcgcgccaa aagcgcccag tgccacaaaa
     7621 atgaagccgc taatggcggc gaaaatcagc ataaaacggc tggtcatggt cgtaccctac
     7681 gtaaattatt gttcataacg aaagcggaat ttttcttgtt cagcagcggc tttcgccagg
     7741 atccattggc gaaaggcggc tattttaccc agttctgcct ggctgtcatg acaaaccaga
     7801 taaaaagcgt ttttactgac cagaacatca ttaaacgggc aaacaagacg tccggcttca
     7861 atttcagatt gcgccatcac gttatttgcc agcgccactc cctgcccgtg aatagccgct
     7921 tgcagcacca tggcgctatg gctaaaaatt ggcccttgct gaacgttgat atgatttaac
     7981 cccaattgac gagtatatgt ctgccagtca cggcgtgacg catcatgtaa taacgtatgt
     8041 ttagccagat cttccggggt cttcaagggt ttttcgccag tcagcagtag cggcgaacac
     8101 accggcaata aatattcggc gtacagtttt tccacccgta gccccggcca gttgccccga
     8161 ccataaaata tcgccacatc aacatcatcc gccagcttat cttcctgacg atcaaccgcc
     8221 tggattcgaa cgtcaattcc cggataagct gaattaaagc tggaaagtcg cggaaccaac
     8281 caatgaatgg cgaaactggg gagtaaactg accgtcaacg cccccttggc gctacgggcc
     8341 tggagtttac gcgtcgcttc ggttaattgc gaaaatatct ctttgatatc gaggaaatag
     8401 ctttgccctt cctcggtcag caggagtgaa cgattacggc ggcggaacag ttttagcccc
     8461 aaaaaatcct caagagactt gatttgatga cttactgcgg cctgggtcac aaaaagctct
     8521 tctgctgcgc gagtgaaact taaatggcgt gctgcggcat caaaaactcg taaggcattt
     8581 agcggtggta atcgtttaga catggctttt aaactttgat gttaaatgaa tttaacaatt
     8641 agatcacact gtgtaaccta ttagtttttt taatctgagc cattataaat tgtccgttga
     8701 gcttctacca gcaaatacct atagtggcgg cacttcctga gccggaacga aaagttttat
     8761 cggaatgcgt gttctggtga acttttggct tacggttgtg atgttgtgtt gttgtgtttg
     8821 caattggtct gcgattcaga ccatggtagc aaagctacct tttttcactt cctgtacatt
     8881 taccctgtct gtccatagtg attaatgtag caccgcctaa ttgcggtgct tttttttacc
     8941 ttgcgatcgc gaattactga tccagttcga ccatctcttt cacgtcggta cggttgatct
     9001 gctgtttgtt gccattagcg tctttatacg aaatcatacc ggtatcgtta tcagtctgtg
     9061 gtttgccgtc agagacgatg gtacgtccgt cattggtgtg catcacatag ttcgaaccgg
     9121 aacaggcgct cagggcaaaa gtcagcatac aggcagaaat aattgcggca gtctttttca
     9181 tcatcttctc cttaaagcga ttaataaatc ttaatagtat tgattaacag gctaaaatta
     9241 acgcctaaca ctattcagca tatgttactg acgcggcttc gccaggatat ccagataatt
     9301 ctgatggtta gcactctcct tgtatcaaag tgaattttgc gtcacgatcg gtgcatcaag
     9361 ccgaggagta ccatgaacgt ttttaatccc gcgcagtttc gcgcccagtt tcccgctcta
     9421 caggatgcgg gcgtctatct cgacagcgcc gcgaccgcgc ttaaacctga agccgtggtt
     9481 gaagccaccc gacagtttta cagtctgagc gccggaaacg tccatcgcag ccagtttgcc
     9541 gaagcccaac gcctgaccgc gcgttatgaa gctgcacgag agaaagtggc gcaattactg
     9601 aatgcaccgg atgataaaac tatcgtctgg acgcgcggca ccactgaatc catcaacatg
     9661 gtggcacaat gctatgcgcg tccgcgtctg caaccgggcg atgagattat tgtcagcgtg
     9721 gcagaacacc acgccaacct cgtcccctgg ctgatggtcg cccaacaaac tggagccaaa
     9781 gtggtcaaat tgccgcttaa tgcgcagcga ctgccagatg tcgatttgct gccggaactg
     9841 attactcccc gtagtcggat tctggcgttg ggtcagatgt cgaacgtaac tggcggttgc
     9901 ccggatctgg cgcgagcgat tacctttgct cattcagccg ggatagtggt gatagttgat
     9961 ggtgctcagg gggcggtgca tttccccgcg gatgttcagc aactggatat cgatttctat
    10021 gctttctctg gtcacaaact gtatggcccg acgggtatcg gcgtgctgta tggcaaatca
    10081 gaactgctgg aagcgatgtc gccctggctg ggcggcggca aaatggttca cgaagtgagt
    10141 tttgacggct tcacgactca atctgcgccg tggaaactgg aagcaggaac gccaaatgtg
    10201 gctggtgtca taggattaag cgcggcgctg gaatggctgg cagattacga tatcaaccag
    10261 gccgaaaact ggagccgtag cttagcaacg ctggcggaag atgcgctggc gaaacgtcca
    10321 ggctttcgtt cattccgctg ccaggattcc agcctgctgg cctttgattt tgctggcgtt
    10381 catcacagcg atatggtgac gctgctggcg gagtacggta ttgccttgcg ggccgggcaa
    10441 cattgcgctc agccgctact ggcagaatta ggcgtgaccg gcacactgcg cgcctctttt
    10501 gcgccatata atacaaagag tgatgtggat gcgctggtga atgccgttga ccgcgcgctg
    10561 gaattattgg tggattaatg acgaacccgc aattcgccgg acatccgttc ggcacaaccg
    10621 taaccgcaga aacgttacgc aataccttcg caccgttgac gcaatgggaa gataaatatc
    10681 gccagttgat catgctgggg aaacagcttc cggctttgcc agacgagtta aaagcgcagg
    10741 ctaaagagat tgccggatgc gaaaaccgcg tctggctggg atataccgtg gctgaaaacg
    10801 gtaaaatgca cttctttggc gacagcgaag gacgtatagt gcgcggcctc ctggcggtgc
    10861 tgttgactgc cgttgagggg aaaaccgccg ccgaattgca ggcacagtcg ccgctggcat
    10921 tgtttgatga gctgggattg cgtgcgcaac ttagcgcctc acgcagccag gggttaaatg
    10981 cgttaagcga ggcgattatc gctgcgacga agcaggttta agcacagaac cgggtcggat
    11041 aagacgtttg cgtcgcatcc gacctgattg tttacccctg acgcgccgct ttcgccatca
    11101 tcttcttcag cgcatgagaa accgcaacaa aaccaaaggt ggcggtcacc atcgttgccg
    11161 cgccaaatcc agatgcacaa tccatccgct ttggtccttc cgccgtggct ttcatcgcac
    11221 acaccgtacc gtctgactgc ggatacacca atgcttcggt agagaacacg caatccacac
    11281 cgagcttacc tttactgttt ttcactacgc caaaatcgct tttcaggcgc tcacgcaact
    11341 tcgccgccag cgggtcctga atcgttttcg ccagatcggt aacctgaatc tgcgttgggt
    11401 caatctgccc acccgcgcca ccggtcgtga ccagcgggat tttattgcgc cgacaataag
    11461 caatcagcgc cgctttgggc cgtacactat caatggcatc aatcacgtac gaataaccca
    11521 cgctcatatg ctgcgctacg ttatccggcg tcacgaaatc atccaccacc gttacacagc
    11581 actccgagtt aatctggcga atacgttccg ccatgacttc cgcttttgcc agcccaacgt
    11641 tatcgcgcag ggcatgaatt tgccgattgg tattggtgac gcacacatca tccatatcga
    11701 taagcgtgat tgcgccaatc cccgtgcgcg ccagcgcttc cgccgcccag gaaccgacac
    11761 cgccaatacc gaccacacaa atatgcgcgt cagcaaacag ttgcaacgct ttttcaccat
    11821 acagacgcgc tgtgccacca aaacgctgac gccatgcatc actaattacc acagacatag
    11881 cacctcagaa ttaaaaaggg cgaggttatc cctcacccta aacgcataaa tgcctgatgc
    11941 gctacgctta tcaggcctac ataactcctg caatattttg gatttgcatg atttgtaggc
    12001 cggataaggc gttcacgccg catccggcat gaacaacgcg cacttagtca ataatcagga
    12061 tgtgaggtta cccctcaccc tgtcatatcc gtaaaaacgg catacagaat atcacatcag
    12121 ccgctaaaga cgttacctgc gcccggggcg gttttcagca cccagacacg tccatagtgg
    12181 ttgtaccaac ctgcgcggtg tccggcttcc ggcccgatcc cttgataaat atcgaagtgt
    12241 tggcctttga ttgcgccacc gacatccaac gccaccatca gacgcaattc gtactgaccg
    12301 ttaaatttgc cgttattatc cagcagcggc acttctgcca gcaaggttgt acctggtggg
    12361 ataatggaac gatcagaggc aacagaagca cggccaacca gcggcaccgc acttgcccct
    12421 ttcaccggtg caaaagattg cggtttaaag aaaacgaaag acgggttctg ttccagcagc
    12481 tcgcgaacct cggcttcact gtgtgtttcg ccccaatgac gaatcgcctg catcgacata
    12541 tcttcttttt tcacttcgcc acggtcgatc agcaccttac caatgctgcg ataggcatga
    12601 ccgtttttcc ctgcatagct gaaaaagtta agcggactgc catcaccaaa gtcgatatac
    12661 ccactaccct gcacatccat aatgaaatta tccatcagag agttgctgta agcgaggatg
    12721 tacttatcgc tcaatgcacc cgcgtagatc tccgcacgag acggcagacg accacgtttt
    12781 ggcggcatac ggtaaatagg atactggaac tcgccctggc gggtatggcg cgcctgaatt
    12841 accggcgtgt aatagcctgt aaactgcacg ttaccatagt tgtcggcacc ttccatctgc
    12901 caggcatcaa tgccgaactg gcgcatattg cgggtatcac cgcctgcgcg cagccactct
    12961 tgcaccgcgt tataaacatt actctggttg ccatacagac gcggtgacga attacggata
    13021 tggttaattt gctcggcaaa atcaccggcg ttaatcggcg cgccaacggc atctggctgg
    13081 ttcaccagag agaaaggctg ggtaaatttc ccgtctttat attgctgtcc gcgatcggtt
    13141 ggtttggaag agcaggcggc aagcattgcc acaaccgtgc ccataagaag gtactttacc
    13201 caacgtcctt tcattgttct cttcttcagg ttaaaaaata aggcgcaacg aagataacaa
    13261 accaccgcgt ttaatgaaat gagcagcctc tccctgacgc aaattttgca caaaaaatag
    13321 gctttagtga tttgtttttg ttcaaaatca tgccagatcc gtgatcgggg taaaaaaaag
    13381 gttgcatgaa aacgcgagcg gagtatagtg cgcatccacg gacgcggggt ggagcagcct
    13441 ggtagctcgt cgggctcata acccgaaggt cgtcggttca aatccggccc ccgcaaccaa
    13501 ttattgaaca ccctaacggg tgtttttttg tttctggtct cctataaaaa agcgccattc
    13561 agcgcctttt tatcatcccc ttctcgccag ggtcgcccca tcggcaaaat acgctttaat
    13621 tcccgcaaga atagactccg caacttcctg ctggaaggtc gccgttttca gtttacgctc
    13681 ttcctcaacg ttactgataa acgccgtttc gaccagaatg gagggaatat ctggtgcctt
    13741 tagtacggca aacccggcct gttcaacttg atttttatgc agcttgttga ttttaccgag
    13801 cttattcagc accgctttac caaacttaag gctgtcagca atggtcagcg actgcaccat
    13861 atcgaacatg gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc
    13921 ggaggcgttc tgggtttgtg ccagatattt tgccgcagta ctggttgcac ctttggttga
    13981 gagcgcaaac accgaggaac cgctcggctg gcgactggta aaggcgtcgg catggataga
    14041 gacaaacagg tcagcacgct gtttctgagc ttttgctacg cgcacttgca acggaatgaa
    14101 gatgtcttca ttgcgcgtca tgtacacctt catattgccc tctttctcga tcagagagcg
    14161 caaacggcga gctatttgca ataccacatc tttttcgcgc gttttgtatt tccccaccgc
    14221 accggagtct tcgccaccgt ggccagggtc aagcataatg acaatcggac gatcgcgccc
    14281 tgctttacct ggttgtggac cactttgcgc tggcggcacc tgcttttcga ggtcgccttt
    14341 gttgtaatcc tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata
    14401 gaggtccatc accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg
    14461 ttttacgttt tgctttaatt caaaaaccat ccgtacggtt tgcgggtcaa attgcccgac
    14521 gcgcgccgac ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac
    14581 cgagttcagg tttacatctt cgatatccac caccacgcgt tccgggttac tcaacgcgaa
    14641 ctgcttatat ttcagctgac gatttgattc taccgtcacg cgggtgtagc tggacgcagg
    14701 ccagacgcgc accgccacga cctggctgac cgcagccaga ctgacctgac ttacgctcaa
    14761 tagccacatg gcacccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc
    14821 tgacatgcct ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg
    14881 accggaaact ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt
    14941 tatattcacg ccattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct
    15001 gcgaaaaaac agaataaaaa tacaataaat tcgaataata atgcaaagag gtgtgccgtg
    15061 gtaaaggaac gtaaaaccga gctggtcgag ggattccgcc attcggttcc ctatatcaat
    15121 acccaccggg gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat
    15181 ttctccagta tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg
    15241 gtctatggcg cacgtccgca gatcgacgca aatctggctg cgcatcacca cgaaccgctg
    15301 tatcacaaga atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg
    15361 ggaacattgc aactggatat tactgctcgc ctgtcgatga gtctcaataa cacgccgcta
    15421 cagggcgcgc atatcaacgt cgtcagtggc aattttatta ttgcccagcc gctgggcgtc
    15481 gatgacggcg tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgatc
    15541 catcgtcaac tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact
    15601 ggcgagagct ttaatctgac ctcggaagag attgccactc aactggccat caaactgaaa
    15661 gctgagaaga tgattggttt ttgctcttcc cagggcgtca ctaatgacga cggtgatatt
    15721 gtctccgaac ttttccctaa cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc
    15781 gattacaact ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc
    15841 gtgcgtcgct gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc
    15901 tcacgcgacg gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca
    15961 acaatcaacg atatcggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt
    16021 ctggtacgcc gttctcgcga gcagctggag atggaaatcg acaaattcac cattattcag
    16081 cgcgataaca cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg
    16141 gaaatggcct gtgtggcagt tcacccggat taccgcagct catcacgggg cgaggttctg
    16201 ctggaacgca ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc
    16261 acgcgcagta ttcactggtt ccaggaacgt ggatttaccc cggtggatat tgatttattg
    16321 cccgaaagca aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat
    16381 ttagggtaag cgatgaaaat cgtcggatgc gacatgcgta acactcgtac gtcgcatccg
    16441 gcaattacgt ttattcccgt gaactaaaca acgccgccag accactgcgc cgctcagtac
    16501 gagtggcgat tgccgcactt aatatgcgct catcggcata cagcgacaga cggcgacgcg
    16561 cgcgggtcac cgcggtataa accagctctc gcgttactac cggcgtgcgt tggctcggta
    16621 aaatcaacgc cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg
    16681 tagtttcgtg ctctggcaag cgactcggtt gcacagactt aatattgccg tccggcatcg
    16741 caaaccagac gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca
    16801 acccaagcgc gctgtcatta cgggcaatca tcaccggtcg gccttcgtac caacgagagt
    16861 gcggattacg atgaattttg cgcttctgtt gcataaactg ctcaattcgc tcattcagtc
    16921 cagccacgcc aaatggccct tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct
    16981 gaatgattaa atccggctcg gcacgcgctt gcagcagatc cagataacgt ccataacccg
    17041 caagagcttc ctcaagcatc gcaatataat cttcgccgct ctgtaaaagc cgtttttcaa
    17101 tatcagtaaa atcctgctga aaaacggttt tcactgccgt tttatcacca cggttgatcg
    17161 ccgcagctaa ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc
    17221 agagactgtc gcgcaaagat gccgcttctg tgccagttcc tgccggaacg tgagttcccg
    17281 tcaggcggct tagctgcccg gcacgctcgg cggtaaagcc ggcattggca taagcgcaga
    17341 tatcgcccag cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga
    17401 tcactcgcgc atgatcgggc aaggcgtcga tcagtctcga catcataggc agatcgatca
    17461 ttgacgcttc atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta
    17521 aacgctggct acccggctgc gcacccagca atcggtgcaa agtactggca tcttccggaa
    17581 tacgtttctt ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg
    17641 ttaagcgcgc ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt
    17701 cggccatttg aattaacgct gccagcaact tcgctacggt ggtcgtttta ccggtgccag
    17761 ggccgccgga aatcaccgag atccgccgcg tcagcgccac tgctgccgcc actttttgcc
    17821 agttaatttc atcaccagtc ggaaagagtt tatccagggt ttgcgccaac aaggcttcat
    17881 ccacctcgat ggcatgattc acctcattga agaagcgcgc taccgtccgc tcgttacacc
    17941 acatgcgatt caaataaaga cgatcgccac agaggatcat cggcgtgggt tcatctcccc
    18001 ggctgaccgc ttgtgaagcc agcaagcatt cttcccaatt ttgtagctca ccgatttcac
    18061 tgacacaggt cgccaacagc ggatgcgact cctcattatt ttccagtcgt gaaagcggca
    18121 aacaaacgtg cccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag
    18181 gatgttcatc tcccgccacg gtcagggcaa actgcacgtc cagcgggcgt agctgtttgt
    18241 gctccacagc ttccagtaat tgcttttgca atttcattac gcctcctcca gagtcatacc
    18301 ggcaaacatc tcatccatca ggtcaatcaa cccggcgttg gggcgggttg tgtaaatccc
    18361 ttgttgcgga tgttctttat caacaccacg caggaacaga taaataacgc cgccaaagtg
    18421 gcgctcatag tcgtaatcag caatgcgatg gcgcagataa cggtgcagcg ccagggtata
    18481 aagctgatat tgcagatcat agcggtgtgc ctgcattgcc gctgccatag cctgttgggt
    18541 gtaagccgaa ctatcttcac ccaaccagtt ggatttatag tcgagcaggt aataacgccc
    18601 ttcgtggcgg aacaccaggt cgataaagcc ttttaacatg ccacgtgcct gcatgaactc
    18661 cagcggcggg cagccagcgg atagcgggtc aaactggcga attagtgcat caagctggct
    18721 ggcgatgagc ggttcgctaa tcggcaggta aaactccatc tccacctgtt tatcgcggtc
    18781 ggaaagctga ctcaggctaa cacccgtttc attgagaggt gcctggagga cagccgtgat
    18841 ccactcggtc aacaccggtt cccactgcga ttcaaagccg ccgagttcca gtttttcctg
    18901 tacccagttc gggtcaaccg gctgggtaaa attgaggtct tcaaacaaac tgtgcaagaa
    18961 tgtccccggt gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac
    19021 gctgaccacg cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg
    19081 accacgctgc tgcaagccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg
    19141 tagcgtcctc gcattcagtt ctgcagttaa agcatcatta acctgccagg gctggttatc
    19201 accagtttgt gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg
    19261 aagccctgcc gcatcttgcg gttccccttt ttgcagcaaa cgcccgaggg cactttggtg
    19321 gacgtcggtg tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact
    19381 gcaatgccaa accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg
    19441 ttcgacctcc gcaagatcga cgctttctgg cgcagcgtta agatccagaa cagcctcaaa
    19501 cgagtggcga tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa taaatggcaa
    19561 ccagaccagc ggatattcca gcccttttga tttatggatc gtgacaatct gtaccagatg
    19621 tttatcactt tcgaggcgca tttgttggct ggaggcatta ctgtctggct cgaggatatg
    19681 ttgcgataac cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgtag
    19741 cagttcgctg atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa
    19801 gttttctgca atgttacgcg ccgacatcag cgcccgcatc atcggcataa cgccacgttt
    19861 gcgccagatt tgccgataac catcgaactc ttcgaccacc gcgtcccacg catgttcgtc
    19921 attattcagc gtttcgatat ccagcgcgtt cagccccatc attgacgttg ccagcgcact
    19981 acgcagggtg ttctcacgtt cgggcgtcat caccgcctgc aacaaccaaa gcatttcctg
    20041 cgcttccaga gtttcaaaaa cactgtcgcg gttcgaaagg taaacggaag ggatttccag
    20101 caacgttaag gcatcgcgca cctgggcggc ctcctggcgg ctgcgcacca gcacactgat
    20161 gtccgaagca cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg
    20221 tccggcttgt agccagtcgc gaatttgcgc agcacttacc tgcgccatgg tactttgata
    20281 gtcgccaacg ccgcaacttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc
    20341 acctttaaag acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg
    20401 tatttcgcga aacatgaacg cgtcatcagt ctggctgaaa agcttgttca cactgttcac
    20461 cattccaggt gcggagcgcc agttggtgtc taaagtgtag tgggcgtgaa cttcactacg
    20521 cgccttcata taagtgaaga tatccgcacc ccggaatgca tatatggcct gcttcgggtc
    20581 gccaatcagc aacaatgcgg tttccggctg atggtgccag atacggcgaa aaattcggta
    20641 ctgctggggg tcggtatcct gaaattcatc gatcattgcc accgggaatc gcgtacggat
    20701 cgccgctgcc aacacctcac cgctttcgct acgcagcgcg gaatcgagcc gacttaacat
    20761 gtcatcaaaa cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc
    20821 agccaatgcg cgggtgatca ccagatcgcg gatcgacaat ggttctgcaa gcagttgatc
    20881 gatcgcctca aacagtggat gtcgcggggt ttcccccccg gccttcgtgc gatcttctaa
    20941 gaaacgctgg gagaattttt ccagcgactc cggcaactga taactgtttc tctcttcttc
    21001 tgcccaggcg ctgatcttgt cgatccattt agcctgattg ctacggttaa acttgcgtcg
    21061 atcaatacca gaagattcga tcagcgcatc cagttcaccc actgcgtcgc gccactgctg
    21121 ttttaccgta tcaatacgcg ccacaatttg cgcgtgacgg gaagccagcg tttcatcatc
    21181 gggcggcggt gctttgataa ccggcgcttc accttgcaga taacgattaa tatcgcgcag
    21241 caacgcctgc ggccctttcc aggtttcaaa gacgacctgg gctatttcac gcggcagcgg
    21301 gtagcagtgg cgacgccaga aatcggcgca ggcctggtaa cgtagcagag actcatcttc
    21361 aatcagctgc tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg
    21421 gcaaaaaccg tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa
    21481 ccacttcgcg gcttgcgctt tatcgtcgat ctcttccagc agacgttcgt acagtggatt
    21541 gtcggtggtt tcacgcagac aggcgatgcg caactcatgg atattgctac ggatacgacc
    21601 acgcaattct gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacggtcag
    21661 cgggcgggga aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat agagcgccgc
    21721 aatcgtaaag gttttgcctg tgccggcaga ggcttcaatc aggcgctcgc cctgaaaggg
    21781 caagcgcaaa ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca
    21841 ttgtttgctg caacgcgctg acgttctccc acactttcca gccttcagga tgcacatatt
    21901 cggctttccc gttctggctg ccggaaatct gcgacagaat agccatgcct tgcggctcga
    21961 ccaccgcctg atggaagaaa tcagcaagtt tttgcggcgt cagcagtttt atctgggcca
    22021 cgattttatc acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttca
    22081 atgcttcttc gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga
    22141 tttgcgcaaa ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg
    22201 ccttgtaacg ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca
    22261 tgccccactg acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt
    22321 cggtacgcaa ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg
    22381 ctgagcttgt gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg
    22441 tgctgttacc ggctttttca aagatgacgg attgtttttt atcgaccact acatctttgt
    22501 ttcgacacca ctctgaacca tcagcgccca actgtttttg cacatcgcgt gccagcgttg
    22561 ttgcctgggc ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta
    22621 aggcgtcacg ataagccagc acctctttca acgtaatgga gggcaaaatt ttacgccgtt
    22681 catctcgcga gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcaa
    22741 acgctttacc cttttctgcg gaatccatca tctggttata ccaggacttc gcctgctcaa
    22801 gctgatcttc cgtagcggta tagctaaagt atccctcgag caatgcctgg aacagctgcg
    22861 gcagacgctg ggtgtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa
    22921 aacttatgcc accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat
    22981 aatcattgag cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca
    23041 aaatcaggct gacatcagct ttgggttcgc tggaaaaata acggcttggc gcatacacca
    23101 cgcgcagatt cgactcatca acaatcagct ctggatggtc atatttcttc tctgacttaa
    23161 tcagcgagaa atcgtcagga atataggggt taagctccgg taaggagagc gcaatattgg
    23221 cagctttttg ctgccagtca gcgaaagttt gttcgctgat tttatcgacc tgatacggcg
    23281 catcgacaaa ataagccgtt ttattgtgcg gctctttcgg gctgatatac cagatacgcg
    23341 cattctgcgg cgtcatcatc gccagacgtt cttttactgc tttagcatcg taccgatcgg
    23401 caatattgac tgcatccagc gtatgctcaa caggaacgcg aatcatggta tctgccagcc
    23461 attcgacgta atccatatca cgggtgattg acggataacg gaagtcgata tccagcacat
    23521 tcgccagttc atcgaagtat tgtttatcga tccctttttc acgtaacaaa ttgaggtagc
    23581 taaaaatagc cgccacaacc tgatcgcgat tcgccagacc tttatcggtt aaagacgcag
    23641 agatcgctaa tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct
    23701 caactaaccc ctgcttttgc agccagtcag acagtgtacc tgggctgcga ttaccaatca
    23761 gataggtaat caattcatcc gttttactgc ggaacttcgc tgagttgtta tcgatgcgaa
    23821 actcaacgcg cagaacttta cgcggcagcg ctgggacgta atgaatgata atgccctttt
    23881 gcgcgtcggt gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc
    23941 gaccaaaagt gtccgccgcc atttttgcca actccggcag cggtttatta ctgtaaataa
    24001 ccgccttcat caaattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca
    24061 ccggattacc aggtttgtcg cttaaagttt cgaggttacc accagaaaac tttgaaccgg
    24121 ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgtgtac
    24181 gcgccatggt taattcagcg ttcactgcat tacgctcacg ttcggcgtat ttcttgtcga
    24241 gcaagggttc tgcaatagca tcggccaggc ggtctaccgc accgggcaag gcgtcgttct
    24301 caacttccag atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt
    24361 gcattttgag atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac
    24421 tcatatgttc aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa
    24481 cgggcaccac cagcgccgat agcgatttaa ctgcctgcgg atcagaaacc agcaagacca
    24541 ccataccgtt atccagacgt atagcctgat actggcggtt atctttatca cttttacgga
    24601 tggtttcctg aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa
    24661 ctaacaacaa taatgctttg aaccaggtgc tgcggggcat tcacggacct cataatcaac
    24721 tttattttct ggccagattc aacaggttaa cgcaaatcat ctgccagaat ttaatcttgt
    24781 gctgcacgag tcagcctatg tttatataac catcagtccg tgactggtgc gcatcataaa
    24841 gtaagcggat agattgcgca atttttatac agcgctcatg actgattaaa gcgaaacagc
    24901 ggtaacagga aacgttgcga ctgttcaacg attgcctcca ttgtctctgg tgttaattgc
    24961 cgccagagcc tttgatacca gatatcatca ccttcgccac tcaccatcat gttgccttcg
    25021 taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca
    25081 tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc
    25141 aatggcgcgg acatcccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc
    25201 tgttcggctg caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt
    25261 tcaccattac caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgc
    25321 gccacactta ataaagaggg acgccagcgc aacaagccat ccggctgcac ctgcggcaac
    25381 cagccagtta tctgcacacc gttgcaagcg agatcaattt ccatactctg ccccggctgg
    25441 cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa
    25501 atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcggcg gaacaagcgt
    25561 tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta
    25621 agtccttcca gaataaatgg ctcggtgtcg gggatttcgc tgtcttcagt acggaagttc
    25681 acctgcaaac gcatctggaa gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt
    25741 tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct
    25801 ttaccagcct ggctggccgc aggtagccat tcacgagcat agctttgtcg ttcgcctggc
    25861 tggtagtttt gcggatcaaa cggcatccgg gtatggaggc aagtaagatg cgcttttacc
    25921 cttgcctcgc tttcatcaca gttgagcgct tcatcgcccg gtagataatg actttgcccg
    25981 atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg
    26041 gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac
    26101 aggtagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg
    26161 tcaaagccca atggcgcaag ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa
    26221 accactttga acggaattga acgcattggc atcagagtac aaatgttaac cggcccggcg
    26281 agaaaacgct ggctgatacg ttcttgatcc aggcgctgtg ccagttcatc acgcaatagt
    26341 gacagcggca ccgcatcgcc atactgcgca cctaaacctt cggcgataat cgcctgccat
    26401 tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gagggcgttg
    26461 agcatatcgc gacaaaccgg caaccactct tccagcggac gctcctgtgc cagcccgcga
    26521 cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag
    26581 ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg
    26641 tagcccaaca acatacgcgt caggccaaat cgccaggtgt gttgtccggt ggcggggagt
    26701 tccagctcgc gaacgttgtc gtcatctatc ccccaacgaa tgccggattc gttgacccac
    26761 tgacgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca
    26821 tccagtaatg ccagcacgtc ttccgacaca aagcggctgt ctggcaacga taacaggctg
    26881 ataaacgctt caagtacagg atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc
    26941 aggtaacgat ccgcaggtgc gctaccaaac acagcctgaa taaacggact gtagctgtcg
    27001 atatcagcca ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc
    27061 gccagcaggc gatcgtgtaa aacttcaact tcacgctgcg ggctatggca aacgtggaag
    27121 gtgatactgc tatccagtgg atcaagcgga cgtttgttat cgctacggga aaactcttcg
    27181 atgttcacac cagcaacggc gcggttttcc agttccagaa tgtcagactg aatgttatgc
    27241 agcaggttat ctggcgtcac atcgacaaag gcgtccagct cctggctgct ctccaggtca
    27301 gaaaggagat aaatgtagtc gcgcccaagc ttgccccatg aggccagcag cgggttgccg
    27361 acatcctgtt caccatcgct gttaaagagc tgcccggcat tttcgctgtc gcgaaatagc
    27421 ggtaattcgt gatcttcaaa actgtgtcgg cgctggcgag tcagtagttt cgccagataa
    27481 gcaggatctt taatgtcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt
    27541 tcaatatgtt tacccagcgc ctggagcgcc tggagataaa caggcggtaa cgcggaaata
    27601 ccgcatataa agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt
    27661 tcgataaagc gctgatagag attggcgcgg tgccagcgcg gttgtccgag ttcatgggta
    27721 tattccacca gcgccttcca caacggcgct tgccaggcct gtgcctctcc cagcccttca
    27781 accaaatgtc ctgtttccca ctgtgccagc cagtccggac gatagaccag atactggtca
    27841 aacaggtccg ccgcttttga ggaaagctgg aacagctttc gcttgtcgct atcgtcagtc
    27901 agataatgcc gcaacagggt aaagtcttcg cgctccagca attgcggcag cagagtcatc
    27961 agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatctc cggtaacacc
    28021 cggacgaaca tatcccagat aaagctcgca ggcagcggaa aatcaatgtt tgccgcaata
    28081 ccaaactttt gcgacagggt catttgcagc cactgtgcca taccggtact ttgcaccaga
    28141 atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac
    28201 gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac
    28261 tgacgattcg ggcaatgcag gcgggtcatc tcgcctgctc tgcccccggg tgaaactagc
    28321 gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac ctgccagttg
    28381 gcaggtggcg aaatcgcgcg caattgcgtt tgctgccaac catgccgcca gagctgttgg
    28441 tactggtttc tgctggtaag actgttcatt aatgttcgct ggaaacccga taacgcagtg
    28501 acaatcatca ccatcaacac catcgccaac attacctccg gcaggctaaa gccttgttga
    28561 ttcttcaggg aagctgacat aacgccccct ctttcagcgg acaaaaatcg ctccagccgc
    28621 gtggcgaaaa aacaattttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt
    28681 cataaccggc aattaataag gcttcattat cttccagtaa acgcaaacaa acccgggcac
    28741 cagtggcagc gtacagtaag cactgaactg cgggctgcgt ctgccaggag tgcattttcc
    28801 cccacgccaa cgccgactga acgatggcct ggcggcgcag tgactgactt tccatgctca
    28861 cgcgagaggc aaaactgcga tcctgctggc tcattccttg taatagcaag ctacccaaaa
    28921 ccagcagcat caggaccaga gccagtgacg aaacaccctt ttcgcggttc acaggttgaa
    28981 tcctgtcacg ctatagctgg catccaccac ggtttgcggt tcagacttac tggcagcacg
    29041 catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt
    29101 gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt
    29161 cgcaccgcgt agcgtttcca acacttgctc cttcagacga aatccaatct ggtcggactc
    29221 ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca
    29281 gtcaccctgt ccgacaattt ccagcccttc gccggtacag atgccatgac aataacccgc
    29341 cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagtgccag
    29401 cttacgggtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccaa
    29461 caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc
    29521 ttttacaggc atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa
    29581 accaccagcc accactcgcc cgttgagttt ttgaagccaa tatgcccggc ccatgcggta
    29641 ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag
    29701 cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tcgccccagc aacggaactc
    29761 acaaggcacc ataacgtccc ctccctgata aaactgatac tgtggtcgcg gttatgccag
    29821 ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccaaagc
    29881 cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa
    29941 atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca
    30001 gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg
    30061 gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg aagaatttaa
    30121 cggagggtaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag
    30181 atagccaccg gcgctttaat gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg
    30241 aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc
    30301 ggttcacggc ttaattgcag atgagtttga tccatatgat tactgtacag gtgcgtatcg
    30361 ccaccggtcc agacaaaatc acccacttcc agatcgcact gctgcgccat catatgcacc
    30421 agtaacgcgt agctggcaat gttgaacggc aggccgagga agacgtcaca ggagcgctga
    30481 taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaatgc atggcacggt
    30541 gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgggaa
    30601 tccgggtcgt ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatct
    30661 ggcgttggcc aggcgcgcca ctgtttacca tacactggtc cgaggtcgcc gttttcatcg
    30721 gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc
    30781 tgcagaaacc acagcagttc atggatgatg gaacgcaggt ggcaacgttt agttgtcacc
    30841 agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg
    30901 gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa
    30961 tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc
    31021 atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac
    31081 tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga
    31141 aacgcgccgt agccaatcag gaacaagcca gacacggctc ccatcgggcg cggtttacga
    31201 atatacaggt tgaggataat aaacagcacc acaccttcca gcagcagctc gtaaagctgt
    31261 gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt
    31321 tgcagcagca aaatatcttc tgtacgggag cctgggaaca gcatggcaaa cgggaagttc
    31381 gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccaaacgccc ggcaccaaga
    31441 ccaaacggaa tgagtggtgc gataaaatca gaaacctgga agaaggaacg tttagtacgg
    31501 cgggcaaaga taatcatcac cacgataacg ccaatcaggc cgccgtggaa agacatgccg
    31561 ccgtcccaga cacggaacag atacagcgga tcggccataa actgcgggaa attgtagaac
    31621 agaacataac caatacgtcc cccgaggaag acgccgagga agcccgcata gagtaagttt
    31681 tcaacttcat ttttggtcca gccgctgccc ggacgattcg cccgtcgtgt tgccagccac
    31741 attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct
    31801 attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac
    31861 cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc
    31921 aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt
    31981 aaaagatatg tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga
    32041 cgctccataa aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta
    32101 cgctgcgcaa gattttctgc ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc
    32161 gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg
    32221 cacatgggat cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct
    32281 tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc
    32341 gccacccggg tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca
    32401 acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact
    32461 tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca
    32521 cgtccggcac gttcaatcag gcggcgtgct tcgtcaactt catcgaggct tgtgaccatc
    32581 ggcaacagaa tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg
    32641 atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga
    32701 ttctcttcgc taatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc
    32761 aaggtgacgg gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct
    32821 tcttccgacg gaaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg
    32881 ccatcaatac ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg
    32941 acttttatac gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga
    33001 cggctaagct caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc
    33061 ggatcgacca gcaattcacc gcgatagcca tcgacgatca gcgtccgacg atgcagcacc
    33121 gaaggctgaa tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca
    33181 gcatgggagt tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc
    33241 tcagcaagag ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg
    33301 ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa
    33361 tcgccagccc gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt
    33421 tcaatgaccg tttttaccgc ccattctgcc accgagcctt tatcaacctc ggcaaacaat
    33481 tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt
    33541 tctttttgtg caccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct
    33601 tccagcgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac
    33661 acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc tacaccaggt
    33721 gctgccggta atgcgcggat tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc
    33781 gactgagaaa gaatcgctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt
    33841 tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt
    33901 tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat
    33961 ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc
    34021 aggccgacga tcccttcatc aaaggcgagc gttacagtgc gaccgcgtgg ttttttcagc
    34081 ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag
    34141 acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg
    34201 cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg
    34261 acttaacctc ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc
    34321 agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat
    34381 gacaccacct gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt
    34441 gtactgctgg tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat
    34501 ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat
    34561 ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct
    34621 acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg
    34681 ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg
    34741 cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta
    34801 cctcaaaata aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca
    34861 ctctctgcag ggcattacac actaacaaca attgaataac tcacagttat gtgcagagtt
    34921 ataaacagag gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg
    34981 aagaactacg gaattactac gggaaaaccc ggagcattct gaataagccc gatatagaca
    35041 ttttaaaata ttcattatta gtcaatgcat tacatgtaat ttaagtaatg aaaaagagta
    35101 attcgtgacc caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta
    35161 tttatccaca gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc
    35221 gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc
    35281 tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacccgtt
    35341 gcagtgttgc gcaactcgat taccggcaac ctaaaaagca atataaatca gataattgaa
    35401 caacgcatgt ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt
    35461 aaccaaggta tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag
    35521 aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac
    35581 ttgccgggct ggttacgcca gagaatttaa aacgcgataa gggctggatt ggcgtgttac
    35641 tggagatctg gctaggtgcc agcgcaggga gtaaacctga acaagatttt gctgctctgg
    35701 gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg
    35761 tttgtgttgc cccgttaacg ggcaatagcg gagtgacctg ggaaaccagc cacgtgcgcc
    35821 acaaactcaa acgcgtactg tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc
    35881 agcgtcgcgt tggatcaccg ttgctgtgga gcccgaatga agaggaagac cggcagctac
    35941 gcgaagactg ggaagaatta atggatatga ttgttctcgg tcaggttgag cggattaccg
    36001 ctcgtcacgg ggagtattta cagatacgac cgaaagcagc gaatgcgaaa gcgcttaccg
    36061 aagccattgg tgcacggggc gaacggattc tgacgctgcc gcgcggcttt tatttgaaga
    36121 agaatttcac cagtgcacta ctggcccgtc attttctgat ccagtagcca tcgctttgac
    36181 ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt
    36241 atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga
    36301 gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa agctccccac
    36361 agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc
    36421 gctgctggca tcaatcgcct gggttacgcg cctgacgaat ccgcttttta caatattcag
    36481 tcaggaaatt tctgcccgtg acttgattct gcttctgggt ggcttgttcc ttatctggaa
    36541 agccagcaag gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt
    36601 ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct tcagcctcga
    36661 ctcggtgatt accgctgtgg gtctgtcaga tcacctgttt attatgatgg ccgccgtggt
    36721 gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgagcgcca
    36781 tccttcggta aaaatgctgg cgctctcttt cctgattctg gtgggcttta ccctgattct
    36841 ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat
    36901 tgcggttgaa agcctcaacc tgattcgcaa caaaaagaac ccgctctgat actccgtacg
    36961 ctctcctgcg ggagggcgta ctcacttctg ttaccctcac agtttaagat tttcctgctt
    37021 tcaagatata taacgtcagt ttataaacag actattatca taggtgggcc aaatgaggat
    37081 aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc
    37141 gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga
    37201 aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg
    37261 atgcaaatta accgtgatga tgtttcgcaa ataattgaac gttaacaaat aaggtcagca
    37321 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
    37381 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
    37441 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
    37501 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
    37561 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
    37621 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
    37681 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
    37741 tccgcgaagc gctgcatgac agcctcaagc gtctgcagac tgattacctc gatctttatc
    37801 aggtgcactg gccgcagcgc ccaaccaact gcttcggcaa actcggttat agctggacag
    37861 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgag
    37921 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
    37981 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
    38041 tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac
    38101 tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
    38161 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
    38221 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gcccctgctc
    38281 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
    38341 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
    38401 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
    38461 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
    38521 tgccagcgca aatagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
    38581 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
    38641 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
    38701 gagcggaacg acaaaaaagc cccccaacac gccaatcagc atcaacaagg cataggctgg
    38761 cagctgctcg tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa ttccggctgg
    38821 catacagcgt gacacggttt ccagcgtaac cagcttcgct gccgcacctg cgccaaccac
    38881 gataccaatc gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatgcccag
    38941 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
    39001 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
    39061 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
    39121 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
    39181 ggcgaccagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
    39241 cgctatggta gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa
    39301 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    39361 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    39421 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    39481 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    39541 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
    39601 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
    39661 gtgcactgac tcactcatcg tgttgttcca cttcgtctac ccagcttttc aacgtgacaa
    39721 agtcaggttt gccgctgcca agtaatggca tctgtttcaa atagcgaata tcacgcggta
    39781 cagcaagctc cggcacgccg tgctcacggg catactgttg caacttatcg cgcgtcagtt
    39841 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    39901 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    39961 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    40021 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    40081 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    40141 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
    40201 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    40261 cgttgataga aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc
    40321 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    40381 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    40441 tcgaagtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
    40501 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
    40561 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
    40621 tgaagtcggc aatcgtttta atctgctcga cattcgccag aatgctttta tggctatgga
    40681 cgacgccttt tggatggcct tcagaaccgg aggtaaaaag gatcagcgcc tcttcttccg
    40741 gctgctgttt aacctgtgcc agacgcggca tcagcaaatg agcgaagatc catactttgt
    40801 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    40861 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
    40921 tgatttcagc cgccgtaata gcactggtca gcccttttac cccagcagtg tagttcatca
    40981 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    41041 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    41101 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaaattg acgtcttcga
    41161 cacatttctt ccctgctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
    41221 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    41281 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
    41341 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    41401 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    41461 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
    41521 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
    41581 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    41641 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    41701 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
    41761 cctgggggtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    41821 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    41881 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    41941 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    42001 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
    42061 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
    42121 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
    42181 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
    42241 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
    42301 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
    42361 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
    42421 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
    42481 taataattca cccaaagcca gcgaagcttc ccggctggct gtgcatagtg caatggagtc
    42541 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
    42601 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
    42661 acaggtggct tatcacaccg gtaatttctt attgattggc aatggttacc acaacgaaca
    42721 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
    42781 tgccaaaatg atcccggatg ccgatttagc ctcattattg aaacaaatgc ccggtatggt
    42841 gctgatcaac cgtatcctgc ctggctttga aaaccgttgt attgctctgg acgatcgtta
    42901 cggtgcctgg ctggctacgc gtcatttaat tcagcaaggt catacccgca ttggttatct
    42961 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
    43021 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggcgaac cagacgaaag
    43081 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
    43141 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
    43201 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
    43261 tccgcgcctg accaccgtgc gttacccaat cgtgacgatg gcgacccagg ctgccgaact
    43321 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
    43381 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
    43441 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
    43501 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctattgt
    43561 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacctg
    43621 ccttcacttc cggcaaggcg cggggttcaa catttccccc ttcctgctgg gtaaagacat
    43681 cgcccgattc acataacggt ccggcgacga cggtttccac cgttggtgcg tgttccagag
    43741 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
    43801 tcagatcgtt aaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
    43861 tccgcacctg agtaattaac acgccagact gcgctaccag gaagcgaccc ggttcaattt
    43921 ccagtttcac agggtggccc aaatggcggg cgatttgctc acgcgcggca ttccacagac
    43981 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
    44041 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgc accatagcac
    44101 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
    44161 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
    44221 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
    44281 ccggattaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgaaca
    44341 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
    44401 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
    44461 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
    44521 gcattaagcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
    44581 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
    44641 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
    44701 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
    44761 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg
    44821 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
    44881 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
    44941 tcagccgcga actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcgtac
    45001 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
    45061 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
    45121 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
    45181 cctttctggt acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
    45241 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
    45301 ctgcgggaac agaacgtacc gaattactct ctttagatga attgtgtgtg ttacctccgg
    45361 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
    45421 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
    45481 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
    45541 gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg
    45601 gtttagtggt gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
    45661 tgcaccgccc gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcggggttac
    45721 cgaaactggt cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac
    45781 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
    45841 caccagtaaa ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag
    45901 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
    45961 ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt
    46021 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
    46081 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
    46141 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
    46201 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
    46261 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa
    46321 atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
    46381 ttcatttatc agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc
    46441 aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga
    46501 gacaaacgcc tcaacggcca ggtgcccgat ctctattaac gaaaaaaggg ccggatgtac
    46561 agcacatccg gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt
    46621 ttgcgttcga tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg
    46681 cccacaaacg caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta
    46741 tcaagcagtg tcaggaaggt cgcgccgata atcatattcg acacccagtt tgtcgtcgtc
    46801 gaacaggtaa taccgaaatc gcggcatttc agcggctgaa tttcagagca caggatccac
    46861 accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag
    46921 agccaggaca agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc
    46981 accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct
    47041 ttatctaccg taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga
    47101 gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgca
    47161 tagtacatga tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga
    47221 aacacagcac gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ttgttttaac
    47281 ttcaggcttt cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc
    47341 atacgcaaca cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta
    47401 tttggcagga agaccaccag aataatcagc aggactgctg gtaaagcaag aacccccaac
    47461 attgcgcgcc agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg
    47521 ccgagtgtga ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc
    47581 atttcagaaa gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc
    47641 acacgagcgg cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt
    47701 acaaacagga tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa
    47761 ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc
    47821 aaacgactgg tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg
    47881 atatcaagac caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata
    47941 cgccgcgtat cacgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt
    48001 ttttcctgcc agcagagagt aagacatagt gaaaaaatac gtgaacaatt cacacaggtg
    48061 tcaggtcgga aacagcataa atatggatta aattgctgcg acatgccgtt atgtgatgga
    48121 t
//
LOCUS       DAZFDC010000019        43276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFDC010000019 DAZFDC010000000
VERSION     DAZFDC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.019"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            106..1008
                     /locus_tag="ACHJ48_001241"
     CDS             106..1008
                     /locus_tag="ACHJ48_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4706841.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNECF"
     gene            1155..2375
                     /locus_tag="ACHJ48_001242"
     CDS             1155..2375
                     /locus_tag="ACHJ48_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4706842.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2348..2977
                     /locus_tag="ACHJ48_001243"
     CDS             2348..2977
                     /locus_tag="ACHJ48_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO4706843.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2978..4138)
                     /gene="ybdL"
                     /locus_tag="ACHJ48_001244"
     CDS             complement(2978..4138)
                     /gene="ybdL"
                     /locus_tag="ACHJ48_001244"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO4706844.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4247..5335
                     /gene="hcxA"
                     /locus_tag="ACHJ48_001245"
     CDS             4247..5335
                     /gene="hcxA"
                     /locus_tag="ACHJ48_001245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO4706845.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGVR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGIGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5345..5542)
                     /locus_tag="ACHJ48_001246"
     CDS             complement(5345..5542)
                     /locus_tag="ACHJ48_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4706846.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5724..7829)
                     /gene="cstA"
                     /locus_tag="ACHJ48_001247"
     CDS             complement(5724..7829)
                     /gene="cstA"
                     /locus_tag="ACHJ48_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO4706847.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVVQAKGAH"
     gene            complement(8010..8423)
                     /gene="entH"
                     /locus_tag="ACHJ48_001248"
     CDS             complement(8010..8423)
                     /gene="entH"
                     /locus_tag="ACHJ48_001248"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO4706848.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDI
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8426..9172)
                     /gene="entA"
                     /locus_tag="ACHJ48_001249"
     CDS             complement(8426..9172)
                     /gene="entA"
                     /locus_tag="ACHJ48_001249"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO4706849.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9172..10029)
                     /gene="entB"
                     /locus_tag="ACHJ48_001250"
     CDS             complement(9172..10029)
                     /gene="entB"
                     /locus_tag="ACHJ48_001250"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO4706850.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(10043..11653)
                     /gene="entE"
                     /locus_tag="ACHJ48_001251"
     CDS             complement(10043..11653)
                     /gene="entE"
                     /locus_tag="ACHJ48_001251"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO4706851.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVVYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQINVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11663..12838)
                     /gene="entC"
                     /locus_tag="ACHJ48_001252"
     CDS             complement(11663..12838)
                     /gene="entC"
                     /locus_tag="ACHJ48_001252"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO4706852.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13027..13983
                     /gene="fepB"
                     /locus_tag="ACHJ48_001253"
     CDS             13027..13983
                     /gene="fepB"
                     /locus_tag="ACHJ48_001253"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4706853.1"
                     /translation="MRLAPLYRNALLLTGLLFSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13987..15237)
                     /gene="entS"
                     /locus_tag="ACHJ48_001254"
     CDS             complement(13987..15237)
                     /gene="entS"
                     /locus_tag="ACHJ48_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO4706854.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            15348..16352
                     /gene="fepD"
                     /locus_tag="ACHJ48_001255"
     CDS             15348..16352
                     /gene="fepD"
                     /locus_tag="ACHJ48_001255"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO4706855.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16349..17341
                     /gene="fepG"
                     /locus_tag="ACHJ48_001256"
     CDS             16349..17341
                     /gene="fepG"
                     /locus_tag="ACHJ48_001256"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO4706856.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17338..18153
                     /gene="fepC"
                     /locus_tag="ACHJ48_001257"
     CDS             17338..18153
                     /gene="fepC"
                     /locus_tag="ACHJ48_001257"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4706857.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTLGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18150..19283)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ48_001258"
     CDS             complement(18150..19283)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ48_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO4706858.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19499..23380)
                     /gene="entF"
                     /locus_tag="ACHJ48_001259"
     CDS             complement(19499..23380)
                     /gene="entF"
                     /locus_tag="ACHJ48_001259"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO4706859.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEQTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAAFWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQHYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMAPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(23377..23595)
                     /locus_tag="ACHJ48_001260"
     CDS             complement(23377..23595)
                     /locus_tag="ACHJ48_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HFO4706860.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(23598..24800)
                     /gene="fes"
                     /locus_tag="ACHJ48_001261"
     CDS             complement(23598..24800)
                     /gene="fes"
                     /locus_tag="ACHJ48_001261"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO4706861.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLISL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            25043..27283
                     /gene="fepA"
                     /locus_tag="ACHJ48_001262"
     CDS             25043..27283
                     /gene="fepA"
                     /locus_tag="ACHJ48_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO4706862.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNDGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDNDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27458..28078
                     /gene="entD"
                     /locus_tag="ACHJ48_001263"
     CDS             27458..28078
                     /gene="entD"
                     /locus_tag="ACHJ48_001263"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO4706863.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28200..28352)
                     /gene="hokE"
                     /locus_tag="ACHJ48_001264"
     CDS             complement(28200..28352)
                     /gene="hokE"
                     /locus_tag="ACHJ48_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4706864.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(28703..28855)
                     /gene="hokE"
                     /locus_tag="ACHJ48_001265"
     CDS             complement(28703..28855)
                     /gene="hokE"
                     /locus_tag="ACHJ48_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO4706865.1"
                     /translation="MLTKYALVAVIVLCLTVPGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            28824..29091
                     /locus_tag="ACHJ48_001266"
                     /pseudo
     CDS             28824..29091
                     /locus_tag="ACHJ48_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29294..30412
                     /locus_tag="ACHJ48_001267"
     CDS             29294..30412
                     /locus_tag="ACHJ48_001267"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO4706866.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            30478..30726
                     /locus_tag="ACHJ48_001268"
     CDS             30478..30726
                     /locus_tag="ACHJ48_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO4706867.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            30791..31159
                     /locus_tag="ACHJ48_001269"
     CDS             30791..31159
                     /locus_tag="ACHJ48_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4706868.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            31253..31906
                     /gene="nfsB"
                     /locus_tag="ACHJ48_001270"
     CDS             31253..31906
                     /gene="nfsB"
                     /locus_tag="ACHJ48_001270"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO4706869.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            32014..33261
                     /gene="ybdG"
                     /locus_tag="ACHJ48_001271"
     CDS             32014..33261
                     /gene="ybdG"
                     /locus_tag="ACHJ48_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO4706870.1"
                     /translation="MQDLISHVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(33329..34705)
                     /gene="pheP"
                     /locus_tag="ACHJ48_001272"
     CDS             complement(33329..34705)
                     /gene="pheP"
                     /locus_tag="ACHJ48_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO4706871.1"
                     /translation="MKNASNVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLAMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(34807..37944)
                     /gene="cusA"
                     /locus_tag="ACHJ48_001273"
     CDS             complement(34807..37944)
                     /gene="cusA"
                     /locus_tag="ACHJ48_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO4706872.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKIQVGPEMRRGIA
                     ELNGEVVGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAID
                     NLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLG
                     GIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITNASVEVGPALFI
                     SLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIP
                     PESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEG
                     DLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVET
                     TIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIK
                     VSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVAD
                     VQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLAD
                     VADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAF
                     SGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLW
                     WMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALH
                     HGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
                     AAYKLMWLHRHRVRK"
     gene            complement(37956..39179)
                     /gene="cusB"
                     /locus_tag="ACHJ48_001274"
     CDS             complement(37956..39179)
                     /gene="cusB"
                     /locus_tag="ACHJ48_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO4706873.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(39195..39527)
                     /gene="cusF"
                     /locus_tag="ACHJ48_001275"
     CDS             complement(39195..39527)
                     /gene="cusF"
                     /locus_tag="ACHJ48_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO4706874.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGFDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSGIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(39551..40933)
                     /gene="cusC"
                     /locus_tag="ACHJ48_001276"
     CDS             complement(39551..40933)
                     /gene="cusC"
                     /locus_tag="ACHJ48_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO4706875.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTVLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            41090..41773
                     /gene="cusR"
                     /locus_tag="ACHJ48_001277"
     CDS             41090..41773
                     /gene="cusR"
                     /locus_tag="ACHJ48_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO4706876.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            41763..43211
                     /gene="cusS"
                     /locus_tag="ACHJ48_001278"
     CDS             41763..43211
                     /gene="cusS"
                     /locus_tag="ACHJ48_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO4706877.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSNARGTRFVIVLPERG"
BASE COUNT        10432 a        11926 c        10944 g         9974 t
ORIGIN      
        1 ttaatcctga gttaagcaaa aaaaacataa tcaataaaaa tatatacgca aaaaacattg
       61 attaagtgaa tatatcatgg aagaaaaata taaccggagt agtgtatgac caatctctac
      121 gacttgaaaa agttcgactt aaatcttctt gtcatatttg agtgtattta ccagcattta
      181 agcattagca aagctgctga atcactatat atcaccccat cagccgtgag ccagtcgtta
      241 caacgcttac gtgcgcaatt taatgaccct ttattcattc gctcaggaaa aggtatcgcg
      301 ccgacgacaa cagggttaaa tctgcatcac catcttgaga aaaatctcag aggccttgaa
      361 caaacaatta atatagtcaa taagtccgaa ctaaagaaaa actttattat tcacggccct
      421 cagctcattt cttgctctaa taatagtatg ctgatccgct gcctacggca agattcctct
      481 gtcgagattg agtgccatga tatcctcatg tcagcagaga atgctgaaga actgttggtt
      541 caccgcaaag ccgatctggt gatcacccaa atgccagtga tcagccgttc cgtcatttgc
      601 atgccattgc atacgattcg caatactcta atttgtagta ataggcatcc acgaattact
      661 gacaatagca cttatgaaca aattatggca gaagagttca cccaattaat atctaaatca
      721 gcaggggttg atgatattca aatggaaata gacgagagat ttatgaatcg caaaatttca
      781 tttcgtggtt cctctttgct gacaatcatt aatagcattg cagttacaga tttactggga
      841 atagtcccct atgaacttta taactcccac cgtgactttc ttaatttaaa agagataaaa
      901 ctagaacatc cgttaccttc aataaagctt tacatttcct ataataaatc ttctttaaac
      961 aatctggtgt tctcacgatt tattgaccgt ctaaatgagt gtttttgata tataacctca
     1021 gacagctact ataactttca cctatttatt cacagcgcga atgaaaaata aaacactctg
     1081 cttttcagtg tttactttat aattaacagt cttatctcac tgatcaccct gtaacgttca
     1141 gagagcgtct tcagatgtct atttataaaa ttcctcttcc gctcaatatt ctggaagctg
     1201 cacgagagcg tattacctgg acactcaaca ccctaccccg cgtttgtgtc tcattttctg
     1261 gcggcaaaga ctctggttta atgctgcatc tgacagccga actcgcccga caaatgggca
     1321 aaaaaatctg cgttttgttt atcgactggg aggcgcaatt ctcttgcacc attaactatg
     1381 ttcagtccct gcgcgagttg tacaccgatg tcatcgaaga gttttactgg gttgcgctcc
     1441 cgcttacgac gcaaaattcc ctttcacaat accaacccga atggcagtgc tgggaacctg
     1501 atgtcgaatg ggtacgtcag cccccgcaag atgcgataac cgatcctgac tttttctgct
     1561 tttaccagcc aggcatgacc ttcgaacaat ttgtacgtga gtttgccgaa tggttttcac
     1621 aaaaacgtcc ggcggcgatg atgatcggca tccgtgcgga tgagtcctac aaccgttttg
     1681 tcgccatcgc cagtttaaat aaacaacgtt ttgccgacga taaaccctgg actacggccg
     1741 caccaggcgg tcatagctgg tacatttacc ccatttacga ctggaaagtg gctgatatct
     1801 ggacctggta tgcaaatcat caaagtctct gcaacccact gtataaccta atgtatcagg
     1861 caggcgttcc tctgcgtcat atgcgaattt gcgaaccttt tggcccggag caacgacaag
     1921 gattatggct ctatcacgtt atcgaaccgg atcgctgggc tgctatgtgc gcacgagtca
     1981 gcggggtaaa aagtggcggc atttacgccg gacatgataa tcatttctat gggcatcgga
     2041 aaatcctcaa gccagaacat ttagactggc aagaatatgc attattgctg ctcaatagca
     2101 tgccggaaaa aacagctgag cattaccgca ataaaatcgc catttatttg cactggtatc
     2161 agaaaaaagg catcgaggtt ccacaaaccc agcaagggga cattggcgcg aaagatatcc
     2221 cctcctggcg gcggatatgc aaagttttac tcaataacga ttactggtgt cgggcattat
     2281 catttagtcc tacgaaagcg aagaactatc agcgttataa cgaacggata aaaggaaaac
     2341 gtcaggaatg ggggatacta tgcaacaacg attaacgcag gatttaacgc aatttctcgc
     2401 cagcttgccg gaagatgatc gcatcaacgc gattaatgaa atccgcatgg cgatccatca
     2461 ggttagcccc tttcgtgaag agccagtgga ttgcgttctg tgggttaaaa acagccaact
     2521 tatgcctaat gattacaacc caaacaacgt ggcaccaccc gaaaaaaaac tgctgaaaaa
     2581 atccattgaa atagatggtt ttacccaacc cattgtggtc acgcatacgg ataaaaatgc
     2641 cctggaaatc gtggatggtt ttcaccgcca tgaaattggt aaaggctcat catcattaaa
     2701 attacgctta aaaggttatt tgccggtgac ctgtctggaa ggtacgcgta atcaacgcat
     2761 cgccgcgact attcgtcata atcgcgctcg tggacgccat caaatcaccg ccatgtcgga
     2821 aatcgtccgt gaactcagtc agttgggatg ggacgataat aaaattggca aagagctggg
     2881 aatggacagc gacgaagtgc tgcgcctgaa gcaaattaac ggtctgcaag agctgtttgc
     2941 cgaccgtcaa tattcccgcg cctggactgt caaataacta cagctggcgc agtcgttcag
     3001 ctgctgccag caacgtcgat tccttcttgg caaaacagag acgaatcagt ttatgtggga
     3061 agggatcggc gcaaaacacc gatagcggaa tcgccgccac gccgtgctcc cgcgtcagcc
     3121 actggcaaaa ctcaacatca tccagggtag aaaccgcgct gtaatccacc agcaaaaagt
     3181 atgtgccttc gcacggtaaa atctccagcc gactttcatt taaggcattt accagaatat
     3241 cgcgcttctg gcgataaaag tccggtaacg caagataatg ctcaggttct gcacgtagca
     3301 tatccgcaat tgccagctgt gccggggtat tcaccgaaaa ggtcagatac tgatgtacct
     3361 tgcgaatttc ggcgctgatg ggcgctggcg caacacaata acccactttc cagccggtca
     3421 tatgataggt cttgccaaac gatgacaccg ccaccgcccg ctcacgcagc tgcggatgcg
     3481 ccagcacact ggcatggccc tgttgtgaaa agttgatgtg ctcgtagact tcatcgctaa
     3541 tgacaaaaat ctcgtgcccg gcgatcgcct gccacaaagc ggcgaaatca gcctgctgcc
     3601 agacagttgc actgggatta tgcggagtgt tgaggatcac cagtctggtg cgctcgctta
     3661 acaatgcggc aaattcctgc cagtcaacgc gaaaatgcgg tggttgcagt gccatacgct
     3721 tcactattcc cccagaaagc gcgatggcgg gggcgtaact gtcatagctg ggatcaaaac
     3781 aaatcacttc atcgccattg cgcaccagtg cggtaatcgc cgcgtataac gcttccgtcg
     3841 cccctgccgt tacggtgata tcgctatcgg catctggttg atagccatac aaacgttccg
     3901 ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt catgggcgcg tattggtttg
     3961 ccccctgggc aacgtggtac gccagccgct cctgtaaata gcgcggacca tcaaaatcag
     4021 gaaagccttg cgacaggtta atcgcctggt gttgctgcgc cagcgcgctc atctgggtga
     4081 aaatagtggt gccaagttgt ggaagtttgc tttgtggaat cagagggtta tttgtcattt
     4141 ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa cacgatgcta gtatttggca
     4201 atcaagacgt ttagatgtct aaataaaaca ataaggacaa cacaacatgc ctcacaatcc
     4261 tatccgcgtg gtcgtcggcc cggctaacta cttttcacat ccaggaagtt tcaatcacct
     4321 gcacgatttt ttcactgatg aacaactttc tcgcgcggtg tggatctacg gcgtacgcgc
     4381 cattgctgcg gcgcaaacca aacttccgcc agcgtttgga cttccagggg caaagcatat
     4441 tttgtttcgt ggtcattgca gcgaaagcga tgtacagcaa ctggcggctg agtccggtga
     4501 cgatcgcagc gtggtgattg gcatcggcgg cggcgcacta ctcgacaccg cgaaagccct
     4561 cgcccgccgt ctcggtctgc catttgttgc cgctccaacg atcgccgcca cctgcgccgc
     4621 ctggacaccg ctctccgtct ggtataacga tgccggacag gcgctgcatt atgagatttt
     4681 cgacgacgcc aattttatgg tgctggtgga accggagatt atcctcaatg cgccgcaaca
     4741 atatctgctg gcggggatcg gtgatacgct ggcgaaatgg tatgaagcgg tggtgctggc
     4801 tccgcaacca gaaacgttgc cgctaaccgt gcgactgggg atcaataatg ctcaggccat
     4861 tcgcgacgtc ttgttaaaca gtagcgaaca ggcgcttgcc gatcagcaaa atcagcagtt
     4921 aacgcaatca ttttgcgatg tggtggatgc cattattgct ggtggtggga tggtcggtga
     4981 tctgggcgat cgttttacgc gtgtagcggc ggcccatgcc gtacataacg gtctgactgt
     5041 cctgccacaa accgagaagt ttctccacgg caccaaagtc gcctacggaa ttctggtgca
     5101 aagcgccttg ctgggtcagg atgatgtgct ggcgcaatta accggggcgt atcagcgttt
     5161 tcatctgccg actacgctgg cggagctgga agtggatatc aataatcagg cggagatcga
     5221 taaagtgatt gcccacacct tgcgcccggt ggagtccatt cattacctgc cagtcacgct
     5281 gacaccagat acgttgcgtg cagcgttcga aaaagtggaa tcgtttaaag cctgacgtgc
     5341 aaatttagca gcagcgcgcg ccgccttttc caccgtagcg cgcgtcctgc cgctcacgga
     5401 aaaactcttc gtaggtcatc ggcgtttgat cgggatggtt aacccgcata tgttcgacat
     5461 agttgtcgta atcaggcata ccaatcatca gcttcgccgc ctgacctaaa tattttccgg
     5521 cttttgccag tgaatcaaac atcttggcct ccgctaacca tgttgctaaa agagtgccgg
     5581 atgcggcgtg aacgccttat ccggcctaca aatgctatca acgcgctcgg tcagccccct
     5641 cgccccatcg gggagagggt tagggtgagg gggaacccac ggcgccatgc caacattcgc
     5701 caacatcccc cctcactctg actttagtgc gcgccttttg cctgcaccac gatctcctcg
     5761 acattttccg gcattggctc atacggcgtt tctttcgccg ttggcttcgg ctctttcaat
     5821 gccgcaagtg ccgtcttaat cgagaacagt gccagaacca ccacgaccac cataaagaag
     5881 atggttaacc cggcatccag acggttgttg aacaccagtt gcgccagctg tgactcagta
     5941 tactgcgatg gaatattacc gctgtcgatc attgcctgga acttattagc aatggccagg
     6001 aagccgactt tcgcatccgg gctaaacgct ttctgccagc ctgcggtcag ggtacaaatc
     6061 agcagccagg ccgttggtac cagcgccacc caggcgtaac gttgacgttt catcttgaac
     6121 aacaccacgg cacagagcat cagcgccatc cctgccagca tctggttggc aataccaaac
     6181 agcggccaca gagtgttaat accgcccagc ggatcgacca caccctgatg caggaagtag
     6241 ccccacgcca gcacgcacag cgccgttgcc agcaggttag caggcagtga atcggtccgt
     6301 ttcaggcccg gagaaaccac gcccagcaga tcctgcaaca taaagcgcgc agcacgcgta
     6361 cctgcatcca ccgccgtcag aataaacaga gcttcaaaca gaatggcgaa gtgataccag
     6421 aacgccacat ccatcatgcc gcccagcgcg ccatgcagaa tgtaggccat ccccaccgcc
     6481 aacgtcggcg caccgcccgc acgggaaatg atcgactgtt ctcccacttc gctggcaatc
     6541 tggttgagcg tatccggagt aatcgcaaag ccccagctac tcaccacttg cgcggcagaa
     6601 gcgaccacat ccgccgtccc tgccggagcc agcaccgcca tcgggctgtt catggcgaag
     6661 tacacgcccg gatcgatgat acaggcggaa accagtgcca taatcgccac gaaggattcc
     6721 attaacattc caccgtagcc gataaaacaa gcctgccctt cgttcgccag catcttcggc
     6781 gtggtgccgg aagagatcag cgcatggaag ccagacaccg cgccacaggc gatggtgata
     6841 aacaggaacg ggaacaggtt accggtccat accgggccag tgccgtcaac aaatttggtc
     6901 aacgcaggca tggtcagcgt cgggcgcata atcaaaatgc ctaccgccag accaacgatc
     6961 gtcccgattt tcaggaaggt agagaggtag tcacgcggag ccagcagcaa ccataccggc
     7021 agcaccgccg ccacaaaacc gtaacccacc agcatccagg tcagctgcac gccggtaaag
     7081 tcaaagtacg gtgcccaggt cggactttct gccacccagc cgccagaaat aatggcgaaa
     7141 atcaggaata ccagaccgat gaccgacact tcaccaatac gccccggacg cagatagcgc
     7201 aggtagatcc ccatgaacag cgccagcgga atggtgaacg caacggtata cgttccccac
     7261 gggctatgag tcagggcttt caccacgatc atcgccagta ctgcaaggat aatgaccatg
     7321 atcataaagc aggccaccag cgcgatcacc ccggcggttg gccccatctc ttctttgacc
     7381 agctcaccca gcgaacgacc gtcacggcgc gtagaaacaa acagcaccat gaaatcctgc
     7441 accgcaccgg cgagaaccac cccggcgagt agccagatca tccctggcag gtaacccatt
     7501 tgcgccgcca gtaccggccc aaccaacgga cctgctccgg caatggccgc aaaatggtga
     7561 ccgaacagca ctttcttgtc cgtcggcaca tagtccagcc cgtcgttatg gcgcaccgct
     7621 ggcgtcatac gcgtcgggtc aaccgccagc acatttttgg cgatatacag accataaaaa
     7681 cggtaagcga tcagatagat acagaccgac gccaccacaa tccacagcgc gttgatctgt
     7741 tccccacgat ttaaagcaat atatcccaga gcaaatgctc ccattacaga gagcactgtc
     7801 cagacgaggt atttccctga tttgttcata gttgttatcc gtgtgcgtgt ccatagagat
     7861 gttacatttt gtttctataa catctttgta cgatttaaca acacagcaac catgtaaatc
     7921 ggagttgaaa ccaatattta acttaacaat gttaactcga tcactccgtt aaccgagttt
     7981 tcctgcaatc tcatataatt ctgtcgggtt catcccaaaa ctgccgtacc cagccgacaa
     8041 gtgcagcaac gccgcccctg ttcatcgaaa acgacgattt cccagctctg attttgccgc
     8101 ccaagatgca gcggctggca gacgccgcgt accttgcctt cagacaccgg gcgatgatgt
     8161 gtggcgttaa gttccgtgcc caccacacac tgcccgtcgc gggtcatcat aaatccggcc
     8221 atcgatccca gcgtttcagc cagcgccgcc gacgcgccgc catgcagcag gccaaacggc
     8281 tgatgagtac ggatatcaac cggcatttcg gcttccagca catcatcgcc cagacgggta
     8341 tacacaattc ccagatgcgc caccattgtg ttatcgctgg tggcgttcag ttcgtcgagc
     8401 gttaaatggc gtttccagat catgcttatg cccccagcgt tgagccgcca tcgaccacaa
     8461 tatcctgtag ggtgatatgg ctggcgaggt cagaggcgag gaacaaaatc gtgttggcga
     8521 tctcttgtgg acgggcgatt ttccccagcg gaatgccgag tttaaactgc tcgccaaagc
     8581 cgcgaatacg ctgttcttcg gcgtcatcgc tcacccacag cgtgcgttgc atatcggtat
     8641 cggtggaacc cggcgaaacc acattacagc gcacgccgct acccgccagt tccagcccga
     8701 cgctcaacgc caggcttttc agcgccgctt tcgatgcgcc ataagcactc ataccaatac
     8761 gcggcgtgtg tgcggcgtcg gacgccacag tgacaatcgc cccgccccgc tgacggcgaa
     8821 actggttcat ggtttgctgg aacaggttaa acgcaccgcc gacattaacc gcaaaagtct
     8881 gctgccagtc ctccttactg agctgatcgg tcgcgcccat gcgcaaaatt cccgccgcat
     8941 tgaccagcac gtccagtcgc tccgtttcag ctaacagtcg ctgacatacc tgcgccacct
     9001 gcgcagcatc ggcaacatcc attacttcgg tcgcaaaggg atattgctcc tgagcgaacg
     9061 cttgatcaaa acctgtaact ttcgctcccg cttcaacaaa cgccagcgcc gtggcgtagc
     9121 cgataccttt ccccgcgccg gttacccaga catttttacc gctgaaatcc attatttcac
     9181 ctcgcgggag agtagcttcc accaggcgtc gatggtcggg tttttcgcca acatgacaaa
     9241 gtcgatatcg ccatgcactt tgcgccagcg cgccgccagc gccatcatgc gcaccgaatc
     9301 cagaccgtag tcgatcaggt tgtcgtcatc gaacggttca tcggactcgt ccagcaacgg
     9361 caggatcacc tcacgcagcg ccgctttgct ggcggggata ggtgctggca gtaattcttc
     9421 ggtcatcacc acccggccag aacgtccggc cacatatttc agcgacatca aatgctcgtc
     9481 acggctgaaa tcggccagcg catccgccac cataaacggt ttaatatcgc gcataaatgc
     9541 gtcggttgcg gtggtcatac agccaatgtg ggcatatacc ccggtaataa tcagctggtt
     9601 acgtccactc tctttgagca tttgctccag cggagaacga tgaaacgcgc tgtagcgcca
     9661 cttcaccagc accgtgtcgt cggcatctgg cgtcagtcga tccaccacct tttgctgttc
     9721 cggcgaacgg gtcaggcccg gcccccacat atcattcaac agcgcccgat cttcatcgct
     9781 ctgctctttc ggctgggcgg tgtaataaac cgggatattg tgctgtttgc agtagtcgcg
     9841 cagcgcagca atattcgcga tcacctgctc catcatcggg cagttctcgc cccagaagct
     9901 gacaaaatag tcctgcatat catggattaa caacgcggca cgttgcggtt caaaggccca
     9961 gtcaacttta ttctgcggaa tatcgtgaga ctccggcagt gcgtaagcct gtaattttgg
    10021 aatagccatc gtgttctctc cttcaggctg atgcgcgtga cgccagccac tgacgtaatt
    10081 gttttttatc gactttcccg accgccgtca gcggaagtga atccacacac tccacgcgat
    10141 ccggtaattt aaattcggca ataccctgtt cacgcaggaa acgacgcacc tgcaccgcgc
    10201 gaagcggctc ttttaccacc agataagcac agcttttttc gcccatcagc tcatcttcca
    10261 tactgaccag tgcggcgtag atcaccgccg gatggcgcag taacaggttt tcgatctctt
    10321 cggcagcgat cttctcgccg ccacggttga tctgatcttt ctcgcgcccc tgcacggtga
    10381 tgtaaccctc tggatcaata gagatcagat cgccggaaca gtaaaaaccg ttggcatcaa
    10441 aggcgctggc attgtgctgc gggcttttgt aatagccacg gaaggtatac ggcccgcgcg
    10501 tcatcagtcg tccgacttcc ccttgcggca gtggatttcc ttcggcatcg gcaacccata
    10561 cttcgtcatc cggacacatt gggtaaccct gggtatggat aattttctcc gcgctatcat
    10621 caagacgggt atagttcacc agcccttccg ccataccaaa cacctgctgc aactggcaac
    10681 caatctcagc gggaatacgc gccgcaagcg tggcagaaag acgtgcgcca ccgacctgta
    10741 acagtttcag cgaggcaagc tgcgcccggc tttcgccttc ggtcagcgcc tgcaaccaca
    10801 ggctgactgc cggcggcacc agcgcggtaa cgttaatctg atgtttttca atcaatggga
    10861 agcaaagcgt ggcgctggga tcggccgcca gaacaaccgt tcctccggca agaaagacgc
    10921 ccagcgatcc cggcgaactc atggcgtagt tatgagccgc cgggatcgcg cacaggtagc
    10981 gtgtctgttg tgtgaactga caaatctcga cgctacgacg cacgctgtag tagtagtcgt
    11041 tatgagtgcg cgggatcagc ttcggtgtac cggtggtgcc gccggaaagc tggaaataga
    11101 ccacttcatc agcaggtgac ggcgtagcag taaaaccgtc tgccggatgg ttaatcgcat
    11161 cctgcaagtt atgctcaccg ctgtcgttga gtagctgcac cacgcgaatg gaagaatgct
    11221 ctgcaacaaa tgtgttgagg aaatcatccc cgctaaacag cgcatgttgg cgatcggcaa
    11281 tcagcaatgc gggttcaatc tggctggcat aggcgttcag ttcactacgc tgatggctga
    11341 acaacgccag caccggcgca acgcccagtt tcagcagcgc gaaaaaggta atgtaaagtt
    11401 cagcgacgtt acccagttgt accagcgcgg tttcaccagg tttaatgccc tgacggcgta
    11461 aactacacgc gaggttatcc gccgcctgat tcagctcccg gtaactcaac tgtcgctcgc
    11521 cgtcgataac cgcgatgctg tcactcgcag cgtggcgagt cagaatgtca gtcagtggca
    11581 aatcctgcca gtagcctttt tcccgatagc gacgggcaaa ctcttccggc cagcgggtga
    11641 atggaatgct catcctcgct ccttaatgca atccaaaaac gttcaacatg gtagaaagtt
    11701 tgacgcctgt ttcgcgccac tcacccaacg gtgacgacgc aggcacaatc cccgctccgg
    11761 caaacagacg cacctgattt tcccgcagct tcgcgcagcg gatggtcacc acccattcgc
    11821 cgttaccttc gctgtcacac caacccacaa tgccgccaaa cagttcgcgg tcgaacggct
    11881 ccagttcagc aataacctgg gtcgcggcct gatgcgggaa accgctcagc gcgggggtcg
    11941 gatgcagcag acaggccaga gtcagtgcgt tttcttgcga attcgcttta ccttcaaagg
    12001 gagttgcgag atgccacagc gtcggcgtgg taatcaattg tggagaggaa ggaacgtgta
    12061 actcactact gcgttcgcgc agtacctctt tcatcgcctg agtcaccagt tcatgttcat
    12121 ggcgatcttt ttctgacgcc agcagacgat tacccgcttc gcgatcgagc acttcatccg
    12181 gctgacgacg tgcggaaccg gctaacggaa tggagctaaa acgctcaccg tctttacgta
    12241 gcagcagttc cgggctggcc cccagcagaa cgccaccatc tgccagcggg acatggaagt
    12301 tgtaactaac cgggttttgc gcaatcaacc gttccagcaa tacgccacta tcaatggcgg
    12361 cgtcagtggt gatatcaatc aaccgtgaca acaccacttt gtcgacctgc ggcgtggcgg
    12421 taagtgcggc ggcgcgggca accatctgtt caaacgtggt ttgctccgga attgcctggc
    12481 gttccaccac gttcagcgac tggctgcggg tgaaacggcg tgcggaagct tgtttttcct
    12541 gacgggagaa cgactgccag gattcaggaa tatacagcga cgaaggctga cgtggatcga
    12601 aaggaatcgc cccgaccatc accggatttt tgatgccctg cgctttggca tcggcaaaca
    12661 gcgcggcgag tttttgctgg aagggactgt cgggcgaatc cccgttcaca gccggttcat
    12721 cgaagcgggc gaaacatcct gacgtcgtaa aactgcggta cggcgacata aagaaaaagc
    12781 gattgggcgc aagtgttgcc atggtctgct gtacttcctc agccagtgac gtatccatat
    12841 catcctccac aaaatgataa aggctttaat aatgattatc atttatattt tcggtcgcta
    12901 acctaaaagc aaacgcgcac tatgtcaact cttgaggtaa cgcaatctgc aactcggggt
    12961 tgcatctggt tgtggttata atgaaaatga gaagcattaa cttcattaat aacaggaggc
    13021 tgatttgtga gactcgcccc gctctaccgc aatgcccttc tattaacagg acttttgttt
    13081 tcaggaatag ccgcagttca ggccgccgac tggccgcgtc agattactga cagccgtggc
    13141 actcataccc tggaaagcca gccgcagcgt attgtttcca ccagcgtcac cctgaccggc
    13201 tcactgctgg cgattgatgc tccggtgatc gccagcggcg cgaccacgcc gaataaccgc
    13261 gtcgcggatg accagggctt tttacgccag tggagcaagg tggcgaaaga acgcaaactg
    13321 caacggctct atatcggcga accgagcacc gaagccgttg ccgcgcaaat gccggatctg
    13381 attttaatta gcgcaaccgg cggggattcg gcgctggcgc tgtatgatca gctttccacc
    13441 atcgccccga cattaatcat caattacgac gacaaaagct ggcaggcgct gttaacgcaa
    13501 cttggcgaaa ttaccgggca tgagaaacaa gcggcagagc ggattgcgca gtttgataag
    13561 caactggcgg cggcgaaaga gcaaatcaaa ttaccgccgc agccggtcac tgccattgtc
    13621 tatactgccg ctgcacacag tgccaatctc tggacgccgg aatcagcaca agggcagatg
    13681 ctggaacaac tcggctttac gctggcgaag ttacccgcag ggttaaatgc cagccaaagc
    13741 cagggtaaac gccatgacat cattcagctt ggtggggaaa atctggctgc agggttaaat
    13801 ggcgagtcac tattcctgtt cgccggtgat cagaaagatg ccgatgctat ttatgctaat
    13861 ccgctgctcg cgcacctgcc tgcagtacaa aacaagcagg tttatgcgct gggaaccgag
    13921 acgttccgtc tggattacta cagcgccatg caagtgctgg aacgacttaa ggcgctgttt
    13981 taagcattaa ccgtcagacg ctgtcacctg cggcggcgtc tggcgaaaac gtcgcaactc
    14041 caccagcacc agcaataaca acacgccgat aatcaataaa ccaaaaccgc tcgcgcttgc
    14101 ggaagcaacc ggtgtcatca tcgctcccaa accacccagc agcgccgcgc ctatggcatc
    14161 gcccgtcacg ttttgtgccg tccacaaacc gttaatccgc cctaacatcg cttccggggt
    14221 ttgcgtttgc agcattgtgt attgcagcaa cgagctgacc gcactcaacc agccgaacag
    14281 cgccagacaa accacgccta aaatccacat cggcatcagg ccaaacagac caatggcgag
    14341 gaacgatccc agcgtggaga gcagcatcaa taaccctggt cgcgcgctat gcgccagctt
    14401 cccgctggtt aacgcgccaa tagccgcgcc gagcgggatc gccgcataga gaaaaccaat
    14461 ctgtgccgcc gacatctgcc agttgtcagc cagcgccgga tacagtaccc gcaccgcact
    14521 cgccatcgtc aataaaccac ccagcagcgc aatccctccc accagcgggc tggcgagtag
    14581 aaaacgaaat cctgccagta atgatttcaa cggatgctca cgcggctgcg gtggcggtgg
    14641 cagtgccgga aggcttaaca acggtagcaa ggtaataaac gtgcccgccg ccgccagccc
    14701 gtagttccag gccacaccgc cagtcgccag cagtaaaccg ccaatcatgg gcgaaatcac
    14761 cgaccccaga cgcacggtca acatggtgat cgccccggcc tgcattaagt tttcacgccc
    14821 taccagtgct ggtgtcgccg ccaatagcgc cgtaacgcca agcgatgcga aaaaaccatc
    14881 ccataaacca agtaaataga ttgccagcaa tgacggctcc ggcagcagcg cgttcaggca
    14941 cagtccaatg aagccaatgc cacaggtgcc gcgcgccagc aaaatcactt ttttgcgctc
    15001 atagcgatcc gccagcacac cgccgaccat caggccaaca aacatcgcgc cgccggtcag
    15061 cgtcaccgaa agccccacct gccaggtgga atgtgtcatc atctggatct gcaccggcac
    15121 cgcgacgccg agcaaaccca gagacacaat tgagatgaaa cgagcgagga atactgcgcg
    15181 aaacgccggg tgcgttttca acaggctgag gttaagcagc caggattgtt tattcattac
    15241 aatgccttgc catcaaatat tttaaatacc tattcttagg ctgcacatgc taacatatcc
    15301 aaataagatc gataacgata attaatttca ttatcatgga agttcgtatg tctggttctg
    15361 ttgccgtgac acgcgccatt gccgtgcccg gattgctgtt attactgatt atcgcgacgg
    15421 cattaagcct gctcattggg gcaaaatcac tccccgcttc cgtagtgctg gaggccttct
    15481 ccggcacctg ccagagcgcc gactgcacca tcgtgctcga cgcgcggctg ccgcgtaccc
    15541 ttgccggttt actggcaggc ggcgcgcttg gccttgccgg ggcgttaatg caaaccctca
    15601 cccgaaaccc acttgccgac cccggcttgc ttggcgtgaa cgccggagcc agctttgcca
    15661 ttgtgctggg tgcggcgctg tttggttact cttccgcaca ggaacaactg gcgatggcct
    15721 tcgccggggc gctggtggcc tcgttgattg ttgcctttac cggcagccag ggcggtgggc
    15781 agttaagtcc ggtgcgttta accctggcgg gcgtggcgct ggcggcggtg ctggaagggc
    15841 tgaccagcgg catcgccctg cttaatcctg acgtctacga tcaactgcgt ttctggcaag
    15901 ccggttcgct ggatattcgc aatctacata ccttaaaagt ggtgctgatc ccagtgctga
    15961 tcgccggagc aactgcgcta ttactgagtc gcgcgctgaa cagtttaagc cttggcagcg
    16021 acaccgcgac ggcgctgggc agtcgcgtgg cgcgcacaca gttgattggt ctgctggcga
    16081 ttaccgtgct ttgtggtagt gcgacggcgg tagttggccc tattgccttt attggcctga
    16141 tgatgccgca tatggcgcgt tggctggtgg gtgccgatca tcgctggtcg ctgcccgtca
    16201 cgctacttgc tacccctgcc ctgctgctgt ttgccgatat catcgggcgg gtgattgtgc
    16261 ccggtgaact gcgcgtttct gtggtcagtg cgtttattgg tgcaccggtg ctgatcttcc
    16321 tcgtgcgacg taaaacgcga ggtggtgcat gatttacgtc tctcgccgat tactcatcac
    16381 ctgtttgctg ctgatttccg cctgtgtggt tgcaggtatc tggggattac gcagcggtgc
    16441 cgtcacgctg gaaatctcgc aggtattcgc cgcgctgatg ggcgatgcgc cgcgcagtat
    16501 gacgatggtg gtcaccgaat ggcgtttacc acgcgtgctg atggcgctgt tgattggcgc
    16561 ggcactgggc gtcagcggtg cgatttttca gtcgctgatg cgtaacccac tcggcagccc
    16621 tgacgtaatg ggctttaaca ccggggcgtg gagcggcgtg ttggtggcga tggtgctgtt
    16681 tggtcaggat ctgacggcta tcgcgctggc agcaatggtg ggcggcatta tcacttcgct
    16741 gttggtctgg ctgctcgcct ggcgtaacgg tatcgacacc tttcggttga ttattatcgg
    16801 tatcggcgtt cgcgccatgc tggtggcctt taatacctgg ctgttgctga aagcgtcttt
    16861 agaaacggcg ctaacagcag gtttgtggaa tgccggatcg ctcaacggcc tgacgtgggc
    16921 aaaaacctcg ccttccgcac ccatcattat attgatgcta attgccgccg ccttactggt
    16981 acgccggatg cgcttgctgg aaatgggcga tgataccgcg tgtgcgctgg gcgtcagcgt
    17041 cgaacgttcg cgtctgttaa tgatgctggt tgcagtggtg cttaccgctg cggcaacagc
    17101 gcttgccggg ccgatttcct ttattgcttt agtcgcgccg cacattgccc gacgcattag
    17161 cggcaccgct cgctgggggc taacccaggc ggcgctatgc ggggcgctgt tactgctggc
    17221 ggccgatctc tgcgcccaac aactgtttat gccgtatcaa cttccggttg gcgtcgttac
    17281 cgtcagcctc ggcggtattt accttatcgt tttgttaatt caggagtctc gcaaaaaatg
    17341 accgaatcag tagcccgttt gcgcggcgaa cagttaaccc tgggatatgg caaatatacc
    17401 gttgcggaaa atctgactgt agaaatacct gatggtcact tcacggcaat tatcgggcca
    17461 aatggctgcg gtaaatccac gttgctgcgt accttaagcc gcctgatgac gcctgctcat
    17521 gggcatgtct ggctggatgg cgagcacatt caacattacg ccagtaaaga agttgcccgg
    17581 cggattggtc tgttggcgca aaatgccacc acgctgggag atatcaccgt gcaggagctg
    17641 gtggcgcgtg ggcgttatcc acatcaaccg ctgtttaccc gttggcgcaa agaagatgaa
    17701 gaggcggtaa cgaaagcgat gcaggccacg ggaataactc atcttgcaga tcaaagcgtg
    17761 gatacccttt ccggtgggca acgccagcga gcgtggatcg cgatggtgct ggcccaggaa
    17821 acggcaatta tgctgctcga cgaacccacg acctggctgg atatcagtca tcagattgat
    17881 ttgctggaac tgttaagcga actgaaccgc gagaaaggct ataccctggc ggcggtgctg
    17941 cacgatctta atcaggcctg tcgttacgcc agccatttga ttgcattgcg agaagggaaa
    18001 attgttgctc agggagcgcc gaaggagatt gtcactgctg aactgattga gcgcatttat
    18061 ggtctccgct gcatgatcat tgacgatccg gtagccggaa cgccgctcgt ggtgccgctc
    18121 ggacgaacgg caccttcaac cgcaaatagt taaactaagt ggtctgccat catggcatcc
    18181 tgttttctgg atgccatcgc atagcgcaat aacacgctac cacaagccac catcccgccg
    18241 atcagcgcgg aaaggatcac aataatcgcc ttacccggac cgtctttttt cactggcaat
    18301 gacgggctta actgatattt aaacggcgta aaattcacat cgttgatatt tgcttttgtt
    18361 aactgctcaa caagatactg acgattacgt aattcaccgt tcagttccgc aacgtcagtg
    18421 accgcttttt caatttccag tttgcgttca ataccgtctg cgccgagaga aatagagaaa
    18481 tcggggtcat ctttaacggc ctgaccatta ctgtaaacgg gctttttaat tcctgccgcg
    18541 ttggcaatgt cgagtgaata attgaggcgc tgaatgtttg catcaagttg atttttcatt
    18601 ttaatgcggt cctgagccag tttttctttt tcaaactggg ttttgatctc cagtttatta
    18661 cggacgtttt ctatcgactc tttcaccacc aacgcagaga tataatcgat atacccgctc
    18721 aaaacggtct gcgcctcttc actggttggc gcggtaaaac ttagcgtcca ggaggtatac
    18781 agtgacggtt catctttttt cttactggca ttgtcatcaa ccgctttcat tttttcgctc
    18841 aatgcgacaa ttgcgcgatg caaatccagt tcgtcgattt tcgcctcttt taattggtcc
    18901 atcacataag gtgatgaacg caggtactct tccagcaagc taaccgactg aaacttcttg
    18961 ataaacaggt taaatgcttc tgtacgatca attttgatat ccagatccag cacacgaagc
    19021 ttagtgaatg ttttctccaa ctcttgccac tgaacaggtt ctggaggcgt gacaaccgcc
    19081 gcgctggtcc atttttgcgg caggatgaaa gagatcagca agcctgcaca ggcaaacgca
    19141 aaaacgaccg ccatgaccgt ttttttggcc cgccataaaa ctgagattag attaagtaaa
    19201 tcaatttcat tattactggg cgacgccaga ggataatcgg gaaaatgagc gtcacttccc
    19261 tgtttaatat tcagtgatga catggggtat aacctgaaaa tgagtccaat agggggaaat
    19321 ctccacggcg ctcactttac cagttggtga gaattatccg aagctgaagt ttgtaaattc
    19381 attccactga atgcaacatt cactctattt aagaaaaata accagcaaac ttataaatta
    19441 aataaatgat aaacccataa ttatttcctg caattatggg tttatagata atgttaattt
    19501 atctgtttag cgttgcgcga ataatcggcc caattttttc aaatgcccct ggagagataa
    19561 tatccacatg cgcacaatcc tgacgataga tatccagctc cgcaatccac ggcgaccagg
    19621 cgcgttcggg gctcatacct tcctgaagcg tacgttcagc aacaaacagc gtcgcttttc
    19681 cgtcaaacgg tacgctatga gcagtcgtca gcaggcgcac agcatcagcg tagttgcctt
    19741 caatggtggt aaacaactcc gttgaagtac ttccctgctg tgccgccagg aaggcctcgc
    19801 gctcgcggtt aatctccgcc agcacttccg ggtccagacc attagcttct ttttcctgcc
    19861 agttttgcgt ttctggcggc caggtatcca gcaagccaag aaatgccacc tgttcgccac
    19921 gggcacgtag tcgcgccgca atgccctgcg ccagtgtacc gccaagggaa taccccagca
    19981 ggtaataagg gccgtgcggt tgttgttcaa gtaacgttgc cagatgcgct tcgcagactt
    20041 catccaggtt cgtcgctgtc tgcatggggc catgagggcg cggcgactga atgccgataa
    20101 tcgaccatag tggatcgaga taacgcgaga gcacgctaaa ctgccaggca aaaccggatg
    20161 ccggatggaa acaaaacagc gtcgggccat taccttcacg caacggcaga atggtttcga
    20221 atcccatgcg ccgggagctg tcctcttcac catcaataat cgttgccagt ttggcgacgg
    20281 ttgacgcgac catcacctgc cccggcgtca cctgacgggc aaactgccga cttaactgcg
    20341 ctgccagttt cattgccagt agcgaatgac cgccaagcgc gaagaaatca gcatcggcat
    20401 cctgcacgtc acaacccagc aacgacgcga atgccgcagc gataatcgtt tcactgcccg
    20461 ctttcggcgc acgccccggc gtttgtgcct taagttcagg caacggtaag gctttgcgat
    20521 ccagcttgcc gttggcgcta agaggtaact gtggaagttg cagcagaacg accggcgcca
    20581 tatgcggcgg caatgtttcg cgaagctgtg cctgtaatgc gctggtatcc aacggcagac
    20641 ctgattgcga caccagatag cccaccaact gacgcgcatc accaccggtg gctgccgcct
    20701 ggttaatcac acaggcgtgg gtaacggctt gttcgacatc cggcagcgcc tgcatcacgc
    20761 gatcgatttc gcccagttcg atacgctgcc cgcgaatttt tagctgatcg tcactgcgcc
    20821 cgaggtactc caccgcgccg ttatccagcc agcgggcaac gtctccggta cggtacatcc
    20881 gttcaccagg gacaaaagga tcggcaatga agcggctggc ggtcagatcg ggtcgtccaa
    20941 gatacccctg cgccagttga ataccggtga gatagagatc tcccgccaca cccggcggca
    21001 ccggatgcat catcgcatcg agaatgcgca agcccgtatt ccacaccgga taaccaatcg
    21061 gcacactgct gccgcgcacc tgtgccagtt cctcgccaaa agccggatac caactcacat
    21121 ctaccgccgc ttccgtcggg ccatatagat tatgcaacgg cgcgcccgtt aactgttgcc
    21181 attcgcggca taaatcagcc ggtaaggcct caccactaca gaaaacctgt ttcaacgtcg
    21241 cgcaattctg gcgagcggtt tgcggcgtca gcgatgcaac aaatgccgcc agcatcgacg
    21301 gcacaaagtg cgtggtcgtt acgccatatt cggcaaagaa ttgctgcata gcgagcgggt
    21361 cgcggtgcgc ttccggttca gccatcacca gtttagcccc ggcaataaac ggccagaaaa
    21421 actcccacac cgagacatca aaactgcacg gcgttttttg ggcaacgaca tcttcacctg
    21481 taagtggata atgattttgc atccacaaca ggcggttaac gatagccgtc tgcccgacca
    21541 ttaccccttt cggcctgccg gtggagccag aggtaaagat gatataagcc gtgtgatgcg
    21601 gttgtgaaag ttgcagcggc gcactgccct gcggtgtaag cggggcgtta tagcaaaggt
    21661 tggttaaatc tggaacatcg gcaaagcgcg gcagttgatc gtcggtggtg attaacagcg
    21721 acggacgcgc atcttccagc atcattttca ggcgatcgtc cggataaccg gtatccagcg
    21781 gtaaccaggc cgcacctgct tcaacaatcg catgtagcgc cagggtcaaa aagaccgagc
    21841 gcggtaatgc caccgccacg ctgtcccctg gtttaacgcc gtgctcacgc agcagattcg
    21901 ccagcgccac cacctgctcg cgcatttccc gatagctgaa ctggtaacgc gcatctgcca
    21961 gcgccggagc atccggtgtt tttgccgctt gttctgccac cagcgcgcta agcgtggttt
    22021 ctggaatctc aacctgagtg gcgttgatct gcgccagctg cgcatactca cctggcagca
    22081 taatatcaac atcgccgcac aacagagccg gatccgcagc gaattgggcg atcagcattt
    22141 tcaggcgttc agcatgctgg attaacgttg gctcatcgta atgctgttta ttggcgagga
    22201 tctcaatact caaatcaccg tgttcatccg ggaacagggc cagttcaagg tcattaaccg
    22261 gaccggttgc cagggtatgg gtttgcgcct gaacaccagg aatatccagt tggtaatcaa
    22321 ataccttgat attgagtacc ggaccaaaca gcggttcatc acctgccgct cgcccgctgt
    22381 cacggacaat ttgttcggca tcgtaacgtt gatgacgacg catttttttc agttgtgctg
    22441 ccagtcgggt tgccagttcc ggcagcgttt cttgtgccgc aatgtgaata cccaacggta
    22501 aaacgttgag cacgggtccg gtagccgtca gcgccgccga gcccagtcga cgcataaaga
    22561 taaatccggc ggcgtagtcc atgcgattgc acaatcgccc cagccaaaag gctgccagcg
    22621 caagggctaa atcggtacgc tgcacacctg aaagttgcgt agccagctgg cggaattccc
    22681 cgtcggtaaa ttccagtttc aggcgtagaa tatctgccga agcgctgcgc cccgctaaag
    22741 gtgccggaga aagtgacgcg ggcggcggca gttgacgacg ctgttccgcc cagaatgccg
    22801 catcacgctg ccaggcttcg ctttcgcggt attgctggta ctcttccact acatcagcga
    22861 aaggcgtaaa cggcgaagca ggcgtttgtt cgccacgcag caatgcgcag taaatgttgg
    22921 cgatctgccg agtaatggcc gggaaactga agccatcgac cagcaaatgg tgataacgct
    22981 gataccagta ccagcggtta tctgccacct gtatcagctg gtgaaagacc agtggtttac
    23041 cgctatcgac gcgcaaatct tgttgcaaat ccgcctgcat taatgcctgc gcagtaccgt
    23101 gcggatcaat attggttcgc aggtcgataa tttctggcag ttcgaatatc agcgcatcat
    23161 cgacccattg ccagacttcg ccgttatctt ccgtaaaacg catccgcagc gtatctgctt
    23221 gcgctaatcc ggcaaccacc gcgcgggcca gtaatggcgc atcaacctct ccggttaact
    23281 caacgtaatg cgccacgctc caggcggagg gtaattctga cagtttttct gccatccaga
    23341 tgccgggctg tgcggcgacc aaaggtaaat gctggctcat tgtgcctcct gcaactgggt
    23401 gaaattcgcc ggtgtcagag tacgccagtg gacttccagc cactgctggc aggactcttg
    23461 tgactgcggc tgacacacaa tgtcccagcc tgccggtaag gcgcattgtt gcggccacag
    23521 actgaattgc ccctgcgcat tgcgcaaaat gtaaaacact ccctgtggat catcgaaggg
    23581 attactgaat gccatattca actcctgtca tggaaaagtg gttgccagag gtcgattaac
    23641 ccctgcatca agccaccgcg ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag
    23701 aaaatggatt cttttatagg gtgtaattgc gcatacagcg cctgattggc ccgcatgatc
    23761 atcggctcgc gaatacccgc ttccagcaca atgcgcagac cttcggcgct aacttcacca
    23821 gcttttagct tttcaagtaa cacgccctct tgctgcccgc cccgatgcgg ccaccagtac
    23881 gatcctgact ggcttaatac acagccaaag cgttcaggcc agtgcagtcc ggcatacagc
    23941 gcggacagcc caccaaaact ctgcccggcg accacggtgc gatcggcgcg atcgctaaaa
    24001 ggggcaatag ctttcaccag gggtaataac tcttgctgta ctgccagcca gaaatccgca
    24061 ttacacggca gttcgtgggc gcggtgcgtg gtgtcgatag cgtcgatcaa cacatacacg
    24121 gcgggaggaa gttgctgacg atgggtcagc gaaatcagca caggccagac gggcatactt
    24181 tgcgcccaaa attcgccatc gagcaaaact gccagcgggc gttcttctgc tgttacatcg
    24241 ccggtggtaa aaatccatac acgacgtgaa tttttcaacc gttcactttt ccagataatt
    24301 tctttggctg gtatttctgg cgcttgcgga caatcccatc ccggttgcag aggcgcttgc
    24361 ggcatttcga gtgcagaaac agcgtgccct cgcccgcctt tccagctttg tggattcagc
    24421 ggatcggcta ttgcctgggg taatagtttt cgccagcctt cacgtaattc gaggcgatcg
    24481 ggggatggtg cagaaaaaat gtcatcgcgt tcggtgggaa taaagcagta gctgccgcgc
    24541 cagttggcat tgagttgtgt cgtccactgc cagacatcag tacctgcaat tcgctgcatc
    24601 gactggggct ggctgttctg atgatgatcg gtcacaccag tgatgtagat ccatacgcgc
    24661 tttatcgtcg agtattcttc agaaccttgg ggatcacgcc accagaaagt gacctcaaac
    24721 atttcgtcat tcagacgctg ccattccggg ccatgtttcg actgccacca gctctcactt
    24781 cctactttta acgccgtcac accataaccc catgtttact gtgcaatttt ttattgattg
    24841 cagaaatata ttgataatat tattgataac tatttgcatt tgcaatagcg taatggcgcg
    24901 ccgtgggaag cgcggacatt aattaaccaa ctgcactgcg tgtctttcag gatcaaaggt
    24961 tttcgcggta gcgggatgcg tcgtgttgat gacgaccatg cccgacagtt gcaattcgtg
    25021 gcaaaaatgc aggaataaaa caatgaacaa gaagattcat tccctggcct tgttggtcaa
    25081 tctggggatt tatggggtag cgcaggcaca agagccgacc gatactcctg tttcacatga
    25141 cgatactatt gtcgttaccg ccgccgagca gaacttacag gcgcctggcg tttcgaccat
    25201 caccgcagat gaaatccgca aaaaccccgt tgcccgcgat gtttcggaga tcatccgtac
    25261 catgccaggc gttaacctga ccggtaactc caccagtggt cagcgaggga ataaccgaca
    25321 gattgatatt cgcggtatgg gtccggaaaa cacgctgatt ttgattgacg gcaagccggt
    25381 aagcagccgt aactcggtgc gtcagggctg gcgtggcgag cgcgataccc gtggtgatac
    25441 ctcctgggtg ccacctgaaa tgattgaacg tattgaagtt ctgcgtggtc cggcagctgc
    25501 gcgttatggc aacggcgcgg cgggcggcgt ggttaacatc attaccaaaa aaggcagcgg
    25561 cgagtggcac ggctcctggg acgcatattt caatgcgcca gaacataaag aggaaggtgc
    25621 caccaaacgc actaacttta gcctgaccgg tccgctgggc gacgaattca gcttccgctt
    25681 gtatggcaac ctcgacaaaa cccaggctga cgcgtgggat atcaaccagg gtcatcagtc
    25741 cgcgcgtgcc ggaacgtatg ccacgacgtt accagccggg cgcgaagggg tgatcaataa
    25801 agatattaat ggcgtggtgc gctgggactt cgcaccattg caatcgctgg aactggaagc
    25861 gggttacagc cgccagggta acctgtatgc gggcgatacc cagaacacca actctgacgc
    25921 ttacactcgc tcgaaatatg gcgatgaaac caaccgcctg tatcgccaga actactcgct
    25981 gacctggaac gatggctggg ataacggcgt gaccaccagc aactgggtgc agtacgaaca
    26041 cacccgtaac tcgcgtattc cggaaggtct ggcgggcggt accgaaggga aatttaacga
    26101 aaaagcgaca caggatttcg tcgataacga tcttgatgac gtgatgctgc atagcgaagt
    26161 taacctgccg attgatttcc tcgttaacca gacgctgacg ctgggtacgg agtggaatca
    26221 gcagcggatg aaggacttaa gttccaacac ccaggcactg accggaacga ataccggtgg
    26281 cgctattgat ggcgtgagtg ccaccgaccg tagcccgtat tcaaaagcag aaattttctc
    26341 gctgtttgcc gaaaacaaca tggagctgac tgacagcacc atcgtaacgc cggggctgcg
    26401 tttcgatcat cacagtattg tcggcaataa ctggagcccg gcgctgaaca tatcgcaagg
    26461 tttaggcgat gacttcacgc tgaaaatggg cattgcccgc gcctataaag cgccgagcct
    26521 gtaccagact aacccgaact acattctcta cagtaaaggt cagggctgct atgccagcgc
    26581 gggcggctgc tatctgcaag gtaacgatga cctgaaagca gaaaccagca tcaacaaaga
    26641 gattggtctg gagttcaaac gcgacggctg gctggcgggc atcacctggt tccgtaacga
    26701 ttatcgcaat aagattgaag caggctatgt tgctgtaggg caaaacgcag tcggcaccga
    26761 tctctatcag tgggataacg tgccgaaagc ggtggttgaa ggtctggaag gatcgttaaa
    26821 cgtaccggtt agcgaaacgg tgatgtggac taataacatc acttatatgc tgaagagtga
    26881 aaacaaaacc acgggcgacc gtttgtcgat catcccggag tatacgttga actcaacgct
    26941 gagctggcag gcacgagaag atttgtcgat gcaaacgacc ttcacctggt acggtaagca
    27001 gcagccgaag aagtacaact ataaaggtca gccagcggtt ggaccggaaa ccaaagaaat
    27061 tagtccttac agcattgttg ggctgagcgc gacgtgggat gtgacgaaga atgtcagtct
    27121 gaccggcggc gtggacaatc tgttcgacaa acgtttgtgg cgtgcgggta atgcccagac
    27181 cacgggcgat ttggcagggg ccaactatat cgccggtgcc ggggcgtata cctataacga
    27241 gccgggacgt acgtggtata tgagcgtaaa cactcacttc tgatgctaac gtcagattgt
    27301 tgacaaagtg cgcgtcgttc atgccggatg cggcgtgaac gccttatccg gcctacaaaa
    27361 atcttgccaa ttcaatatat tgcaggacca tgtaggcctg ataagcgtag cgcatcaggc
    27421 agttttgcgt ttgtcatcag tctcgaatat ggtcgatatg aaaactacgc atacctccct
    27481 cccctttgcc ggacatacgc tgcattttgt tgagttcgat ccggcgaatt tttgtgagca
    27541 ggatttactc tggctgccgc actacgcaca actgcaacac gctggacgta aacgtaaaac
    27601 agagcattta gccggacgga tcgctgctgt ttatgccttg cgggaatatg gctataaatg
    27661 tgtgcccgca atcggcgagc tacgccaacc ggtgtggcct gcggaggtat acggcagtat
    27721 tagccactgc ggggctacgg cattggccgt ggtttcccgt caaccgattg gcgttgatat
    27781 cgaagaaatt ttttctgcgc aaacagcgac cgaattgaca gacaacatta ttacaccagc
    27841 agaacacgag cgactcgcag actgcggttt agccttttct ctggcgctga cactggcatt
    27901 ttccgccaaa gagagcgcat ttaaggcaag tgagatccaa actgatgcag gttttctgga
    27961 ctatcagata attagctgga ataaacagca ggtcatcatt catcgtgaga atgagatgtt
    28021 tgctgtgcac tggcagataa aagaaaaaat agtcataact ctgtgccaac acgattaatt
    28081 gacaacatct ggtacgattc gcccgcagcc atcactgact gcgggcgaaa gtgtaaagca
    28141 ggtgccttac cactctgacc tgacaaccgg atatgcgggg attgctcccc gcacaacggc
    28201 tacttcttcg gttcgtaagc gagaacagcc ttaaactcaa tattacgttc ctttacggtg
    28261 aactcacaca gcgagtctcc gaccagaagc gtaaatccca gcaccgtcaa acacagcact
    28321 atgactgcca caagggcata tttcgtcagc atgtttatat tgcctccatg cgaagaaggg
    28381 actaccatcc gagttgttgg ggctcggagt ggcagcccca ggattgatag aaatatctcc
    28441 tggggcttca tctttccaga cctcagagtt aacctgaaac cggaaagctt caggcacccg
    28501 ccgttatctt acccttcctt agcaaaatag ctgtttattt atacagtgaa tcctgtttcg
    28561 gacttctgtc ggcaaaactc atcattgctc ttaaaacgaa atcgcccgca gccatcactg
    28621 accgcgggcg aaagtgtaaa gcaggtgcct taccatcctg acctgacaac cggatatgcg
    28681 gggattactc cccgcacaac ggctacttct tcggttcgta agcgagaaca gccctgaact
    28741 caatattacg ttccttcacc gtaaactcac acagcgagtc tccgaccaga agcgtaaatc
    28801 ccggtaccgt caaacacagc actatgactg ccacaagggc atatttcgtc agcatgttta
    28861 tattgcctcc atgcgaagaa ggaactacca tccaaattgt ctggtttgga gtggcagccc
    28921 cagggttgat agaaatatct cctggagctt catctttcca gacctcagag taaacctgaa
    28981 accggaaagc ttcaggcacc cgccgttatc ttacccttcc tcaacaaaat agctgtttat
    29041 ttatacagta aatcctgttt cggacttctc actcctcatt taatcaactg aagctgagcc
    29101 cgttttttct gcatgcctca atcgtcaaaa ttcttcgcca ccagccagcc gtgccataac
    29161 ttgtcacatt gcaaaagtag ccaaattgct tcagtcacca tcaccaaaaa aagaaacatt
    29221 ccagaacttc cgcatctcgc ccagccgctc cgacttctat actgaataga aaactccaac
    29281 ataagagaaa cctatgccat tacccgattt tcatgtttct gaacctttta ccctcggtat
    29341 tgaactggaa atgcaggtgg ttaatccgcc gggctatgac ttaagccagg actcttcaat
    29401 gctgattgac gcggttaaaa ataagatcac ggccggagag gtaaagcacg atatcaccga
    29461 aagtatgctg gagctggcga cggatgtttg ccgtgatatc aaccaggctg ccgggcaatt
    29521 ttcagcgatg cagaaagtcg tattgcaggc agccgcagac catcatctgg aaatttgcgg
    29581 cggtggcacg cacccgtttc agaaatggca gcgtcaggag gtatgcgaca acgaacgcta
    29641 tcaacgaacg ctggaaaact ttggctatct catccagcag gcgaccgttt ttggtcagca
    29701 tgtccatgtt ggctgtgcca gtggcgatga cgccatttat ttgctgcacg gcttgtcacg
    29761 gtttgtgccg cactttatcg ccctttccgc cgcgtcgcca tatatgcagg gaacggatac
    29821 gcgttttgcc tcctcacgac cgaatatttt ttccgccttt cctgataatg gcccgatgcc
    29881 gtgggtcagt aactggcaac aatttgaagc cctgtttcgc tgtctgagtt acaccacgat
    29941 gatcgacagc attaaagatc tgcactggga tattcgcccc agtcctcatt ttggcacggt
    30001 ggaggttcgg gtgatggata ccccgttaac ccttagccac gcggtaaata tggcgggatt
    30061 aattcaggcc accgcccact ggttactgac agaacgcccg ttcaaacata aggagaaaga
    30121 ttacctgctg tataaattca accgtttcca ggcctgccgc tatgggctgg aaggcgtcat
    30181 taccgatccg tacactggcg atcgtcgacc actaacggaa gacaccttgc gattgctgga
    30241 aaaaatcgcc ccttctgcac ataaaattgg tgcatcgagc gcgattgagg ccctgcatcg
    30301 ccaggtcgtc agcggtctga atgaagcgca gctgatgcgc gatttcgtcg ccgatggcgg
    30361 ctcgctgatt gggctggtga aaaagcattg tgagatctgg gccggtgact aagccgggaa
    30421 ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa caatgttatg
    30481 aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc tttcctctct
    30541 tgcctgctgc tgcccgcccc cgcactgggg cttacgctgg cacaaaaact ggtgaccacg
    30601 ttccatctga tggatcttag tcagctttac actttattgt tttgtctgtg gtttttagtg
    30661 ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt ctcgctggcg
    30721 gattaaacgt cgattatcgc cgtacttcgc ggcatacttt gcttattctc cttcgcctaa
    30781 aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctgg cccttgagtt
    30841 accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta aaattggcgg
    30901 caagattttt atgctgtcgt cggagctacg cggcgtcccc tttatcaatc tgaagtccga
    30961 tccacaaaaa tccctgttaa atcagcaaat atacccaagc attaagccag ggtatcacat
    31021 gaataaaaag cactggattt cggtgtatcc cggcgaggaa atctccgaag cgttacttcg
    31081 cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac gcgatcaaaa
    31141 aagagtgcgt ccaggctaaa gcggaaatct atagcgcatt tttctcgctt accatttctc
    31201 gttgaacctt gtaatctgct ggcacgcaaa attactttca catggagtct ttatggatat
    31261 catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagca aaaaacttac
    31321 cccggaacag gccgagcaga tcaaaactct cctgcaatac agcccatcca gcaccaactc
    31381 ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg ttgccaaatc
    31441 cgctgccggt aattatgtgt tcaacgaacg taaaatactt gatgcctcgc acgtcgtggt
    31501 gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg accaggaaga
    31561 tgctgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag gtcgcaagtt
    31621 cttcgccgat atgcaccgta aagatctgca tgatgatgca gagtggatgg caaaacaggt
    31681 ttatctcaac gtcggtaatt tcctgctcgg cgtggcggct ctgggtctgg acgcggtacc
    31741 catcgaaggt tttgacgccg ccatcctcga tgcagaattt ggtctgaaag agaaaggcta
    31801 caccagtctg gtggtagttc cggtgggtca tcacagcgtt gaagatttta acgctacgct
    31861 gccgaaatct cgtctgccgc aaaacattac cttaaccgaa gtgtaattct cccttgccgg
    31921 gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg tttcagccgt
    31981 catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc ccatgttgaa
    32041 gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt cggtattatt
    32101 tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg gaccttcgaa
    32161 aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa taaactcttc
    32221 caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt attctggctg
    32281 caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg gatcatgatg
    32341 tatgcgctgc tttcagtctt ctcgctgctg gatgttattt tgaatctggc gcagaaattc
    32401 ccggcagcat ctcagttacc gctgaaaggg atatttcagg ggattaaact gatcggcgcg
    32461 attctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc gattctgatc
    32521 agcggtcttg gtgcaatggc tgccgtgctg atgttggtat ttaaagatcc gattcttggt
    32581 ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga ctggctggag
    32641 atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac cgtcaaggtg
    32701 cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc tgactccttt
    32761 aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag tatcagtatt
    32821 gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa taaagcgcat
    32881 ttgttaaagc cttatttaac cagccgccat caggaaatta atgagtggaa tcgccagcaa
    32941 ggttctacgg agtcggtatt aaacctgcgc cgaatgacca atattggaac ctttcgtgcc
    33001 tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac cttaatggta
    33061 cgccaactgg ctccaggtga taacggttta ccgctcgaga tctatgcgtt taccaacacc
    33121 gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat atttgcgatt
    33181 gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat tcgctctctg
    33241 gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg agagcggaca
    33301 tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta aatgctacaa
    33361 acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca tccatcgtgc
    33421 acatcagcag caaaatcatg gcgaggaagg cgatgcaaag atagttgccg aacggataga
    33481 gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat cgctgcacga aaacgcagat
    33541 gcgccagaca gatcataatc cagttcaaca gcagcgttgc taccaccagc gccatcagca
    33601 gaccaaacgc tttttgcggc agcagatagt tgattaacac caccagcgaa gtgatcgctc
    33661 cggaaagcat cagcgagtta atcggcacgc cgcgacggct aacacgggta aggaacttcg
    33721 gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa accccgctgt
    33781 tatacactga cagcgatgct accagaatga cgaagttcag cgcagaagct accacgttgc
    33841 tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact tccacccacg
    33901 gatagagcgc cagtaaaacc accagtgaac cgatgtaaaa cagcaggatg cgatacacca
    33961 cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg gcagtaatcc
    34021 caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa atcagcccat
    34081 tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt gtcgatactg gctttctcgc
    34141 cgccgtgacc agaaaacagc agccacaggc caaagccgat cataccgatg attgccagca
    34201 ctttaatcag cgcaaaccag aactcggttt cgccatataa gcgcacgttc accaggttaa
    34261 cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca tccgggaacc
    34321 agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc acgaacatca
    34381 cccagtagtt ccagccggag aggaacccgg cgaacggtcc ccagtattta taggcaaagt
    34441 gggcaaatga accggatacc ggctcttcga ccaccatctc gccgagctgg cgcataatca
    34501 ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc gccatctgaa
    34561 tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc gcaatcagtt
    34621 gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac gcagtatctt
    34681 ccgatacgtt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg ttgaggggca
    34741 ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa accgggtatc
    34801 ctgcttttat ttccgtaccc gatgtcggtg cagccacatc agcttatacg ctgccgggat
    34861 aataaacagc gacagcaaag gcgcggtgat catgccgcca atcatcggcg cggcaatccg
    34921 gctcatcacc tctgatcctg cccccgttcc ccacagaatc ggcagcagac cagcgataat
    34981 caccgccacc gtcatcgctt tcgggcgcac gcgcaggacc gcgccgtgat gtaacgcctc
    35041 atccagcttc tgctcgctga atgtttgcgg attattcaac gacggctcgg cctctatggc
    35101 gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg gcgaccccgg caagggcaat
    35161 aaaaccggtg cccgtcgcca cagaaagatg aaagcccatc caccacagca accagatgcc
    35221 gcccaccagc gcaaacggta cgctgctgat aatcagcaac gcttcgccca cccgacggaa
    35281 cgccagatac aacagcacga agatgatcat caacgtcatc ggcaccatga gtttcagctt
    35341 atggttggcg cgctccagta gctcgaactg cccggagaat gccacgctgg tgcccggttt
    35401 taactgcact ttttcagcta tcgctttttg caaatcgtga accaccgaca ccatgtcacg
    35461 atcgcgggca tcgatataaa tccagctcgt cgggcgcgca ttctcggttt tcagcatcga
    35521 cggtccggta gagactttaa cgtcggccac gtctgccagg gtgatttgct gcttcatcgg
    35581 cgtcaggatc ggcaactggc gcagtgcctg cggactatcg cgccagcttt gcggataacg
    35641 cagattaatt ggataacggg caatcccttc caccgtttcg ccaaccatcg ccccgcccac
    35701 cgcagaagtc acaaacaact gcacatccgc caccgtcata ccgtaacgtg cggctttttc
    35761 acggttaatc tcaacgttga tatagcgccc accttccagc cgctcggcaa gcgcagaagc
    35821 cacgcctggc accgttcgtg ccacctcttc aatttgctcc gccatcgcgt cgatatccgc
    35881 cagcacagtg ccggaaactt ttatgccgat agggctttta atgccggttg agagcatatc
    35941 gatacggtta cgaattggcg gcacccacag attcgccagc cccggcagac gcacggtgtt
    36001 atccagttcc tcaatgattt tgtccattgt catgcctggc cgccactgct cctgcggctt
    36061 aagctggatg gtcgtttcta ccatctccag cggagcagaa tcggtggcgg tttccgcttt
    36121 cccggttttg ccaaataccc gcgccacttc aggtacgctc ataattagct tgtcggtttt
    36181 ttgcagcata ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg gcatatacaa
    36241 caagtcgcct tcattgatct gcggtaaaaa ttccccgcca actttattga gcggccagag
    36301 aaccgtcagc accgaaagcg ctgccaccaa cagcgtggtt ttcggccagt gcagtacttt
    36361 cagcaacagc ggatgataaa cacgaatcaa aaagcgattg agcgggttac tgctttccgg
    36421 cggaattttg ccacggatcc agtaacccat caggataggg atcactacga tcgccagcag
    36481 cgccgcaccc gccatcgcat acgttttggt gaacgccagc gggccaaaca gacgcccttc
    36541 ctgcccttcc agggtgaaga tcgggataaa cgacagcgtg ataatcagca gactgataaa
    36601 cagcgccggc cccacttcaa cagatgcatt ggtaatcacc tgccagcgcg ttttattatc
    36661 cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc cgtttatgtg cattctcgat
    36721 catgacgata gcggcatcga ccatcgcccc gacggcaatc gcaatgccac ccaacgacat
    36781 aatattggca ttcagcccct ggaagtgcat gacaataaaa gcaatacaca accccagcgg
    36841 caacgaaata atcgccacca gcgccgagcg cacatgccag agaaacagcg cacagactac
    36901 cgccaccaca ataaactctt ccagcaactt gccgctgagg ttgtcgatgg cccggtcaat
    36961 gagctggctg cgatcgtatg ttgtaactat ctccacgcct tccggcagac tgcttttcag
    37021 cgtttccagt ttgtccttca cggcggcgat cacttcacgg gcgtttttgc cggagcgtag
    37081 gatcaccacc ccgcccacaa cttcgccgtt cagttcggca atgccccggc gcatctccgg
    37141 gccaacctgg atcttcgcaa catcacgcag ataaacaggc acgccatttt cactggcttt
    37201 taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata
    37261 ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc
    37321 gctttttact tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgataaccac
    37381 ctgatactct ttcaccacgc cgcccaccga cgccacttcc gcaacgtcag ggatggtttt
    37441 tagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggccagat cgtgcttacc
    37501 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag
    37561 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
    37621 cgaacgcgcc cagtacggat cggtaccatc ttcgaaaatg acatacacat aagagtcgcc
    37681 aaactgcgag aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtagttag
    37741 cggataagtc acctgatttt caacgatttg cggtgcctga ccgggatagc tggttttaat
    37801 aatcacctgc acatcggaga gatccggcaa cgcatccact ggcgtgttaa tgatggtcca
    37861 ggtgccccag atactcagaa ataacgcgcc catcagcacc aaaaaacggt tcgccaccga
    37921 gcgacgaata atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca
    37981 gagcgcatcc gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg
    38041 ctggaaacca ccttttcacc ttccgccaga ccagagcgta atgcggtgac accttgcgat
    38101 gcctggaaaa cggcaacgcg ttttggtaca aagcgcccgt cggcatcaac ggtaatcacc
    38161 cgctgttcgc tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
    38221 gcggtattga gttgcaacca ggcgttcatt cccggtttta gcgcctcgtc ggcgttgtcg
    38281 acttccagac gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag tagcgtccat
    38341 ttgcggatgg tgagtgtttt atcaggtcgc gccggaacgg tgagcgtaaa ctgcgaggca
    38401 tctttcacca gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata
    38461 ccctgaattt tcgctaccac gttatctttg gcgatattca ttcccgcgcg cagatcaaac
    38521 gcggtgatca cgccatcaat gggtgctttg agcgtaaagc gagtctggat tttttgcgtg
    38581 gcgatcaggc ggcgaatatc cgcctccggc attcccgcca gtcgcagccg ctcaagaatg
    38641 ccttcggtct gggtcgccgt accgccggtt tcgcgcagca gtaaatactc actctgcgct
    38701 tccacccagt cgggaatggt caggtcgaga agcggcgcgc ccttttgtac tttatcgccc
    38761 acggtaagcg gatacacctt gtcgataaac ccggcggcgc gagcctgcac aatggcatac
    38821 tgatactcgt tgtaactgac attcgccggg aaactctggg caaaagtcag cggcccgcgc
    38881 gtgacggtag ccgttttcac ccccagattc tgcgtctgag tcgggtcaat gcgcacacca
    38941 gacgcagaac tctcttcatc ggcatatttc ggcaccagat ccatatccat aaacggcgat
    39001 ttacctggtt tatcgaaccg cgtattggga tacatcgggt cgtaccagaa taagatttta
    39061 cgttctgcgg tcgacgtttt ttctgcaggc ggttccgcct ttgcaaacca ggtaaaacct
    39121 gccgcagaaa taataccgcc cgcgatcatg ctgccgataa taagcgcgat ttttttcatc
    39181 tcattaaacc tgggttactg gctgacttta atatcctgta ataaagaaag gttgccctgc
    39241 tggacaaaat taaacgccac tttgtcgccg gttttaattc cactcatttt cgtctgcggg
    39301 gtgatggtaa agcgcatggt catctccggc cagttcacgg cagcaatcgg atcgtgatgg
    39361 atggtgattt ttttgctttc cagatcaaaa ccttttacca cgccggtggc actaatgacc
    39421 tgtggttgtg cttcgctcat ggtttcatga tgatgttcgt tagcctgggc attaaagcca
    39481 ataacggtaa acagactgaa cattgcgact tgcagtgctt ttttcattta ttttctcctg
    39541 gagttaaaaa ttattgctgc caaccgccac caagtgcggt atataaagaa atttcgttga
    39601 cctgacgggc ataattcagg tcaagtacgg tttgtcgggt tgcaaataaa gaacgctcgg
    39661 catccagcac ttccagataa cttactgcgc cgtgctgata taatgcccgc gcccgttgca
    39721 aagtaatttg cagcgatgcc agataacgct gctgggcgct aatttgatcg ttcaggcttt
    39781 gacgtaatgc cagcgcatct gccacttctt taaaggcgtt ctggattttc tgttcataat
    39841 tcactaccga ctgctgctgg cgaatttcgg cgatatccag attggcctgg ttgcgtccgg
    39901 cattaaaaat ggggatctca attttaggaa taaaattcca catcccgctg ctggcgttaa
    39961 ataatgacga cagatcgctg ctggcggttg atattccgct ggtcaggctg atagacggga
    40021 aaaatgccgc gcgtgcagca ccaatattgg cattagccgc cattaacgca tgttcagctt
    40081 ccataatatc cgggcgctgc aataagattt gcgacgacaa gcccgccggt aatttaacgc
    40141 tttgcaggct gtcgctgttt actgtctgcg cttgcggcag cttgccgtag cttcccagta
    40201 acagttgcaa tgcattattc gcctgcgcca gttccccctg acgtttagcg atgtcgctgc
    40261 gggtactttc aatcaccccg cgagcctgtt ccagcgccag aacattactg ctacctgtca
    40321 acagttgttt ttcgacaaac gcatatgact gctgataatt acgcagcgtt tcttcggcta
    40381 tttgcaattg cgcatacgcc agctgctgat tgaaatagct ttgcgcgaca ttagaaacca
    40441 gcagaatatg caccgcgcgc tgagcttcct cagtggctaa ataattttgt cgctcggctt
    40501 cgctcatgtt ctttaagcgc ccgaaaaaat cgaggtcaaa gctggcgtta aggccagtag
    40561 aaaactcccg cgtcgtggct gaatcgcctt taagattgcc gctccagctg ccgctgccct
    40621 caccattgag ctgtgggtag cggtcggcat cagtcagacg atattgcgcc cgtgcctcct
    40681 gcactttcag cgtcgccatg cgcaaatccc ggttattctc cagcgcctcg ctaatcagcg
    40741 tcttcacctg attatcaaca aaaaaggtgc gccagcccgc gttctgatag ttatctgctg
    40801 cgttaaccag gccgttctgg ctgagtgaga actgctgcgg tacgggcatt gccgggcgct
    40861 gataatccgg tgccagtgaa caaccggtta gcgcaagggc cacacaaaat ggcagaagtt
    40921 tacaaggaga cataggctca taatttctgg tgattttata ccgccaactt tactcgccag
    40981 gctctgattt tccggtgaca ggaaaatgac aaaattgtca ttttgccaat aagcgattgc
    41041 catctgatcc cgctactcta gaattgcccg ggcaacatgc ggaggaaata tgaaactgtt
    41101 gattgtcgaa gatgaaaaga aaaccggaga atacttgacc aaagggttaa ccgaagccgg
    41161 ttttgtggtc gatttggccg acaacgggct gaatggctac catctggcga tgaccggaga
    41221 ttatgacctg ataatcctcg atattatgct gccggacgtg aacggctggg atatcgtgcg
    41281 catgttgcgc tccgccaata aagggatgcc gattctgttg cttaccgcgc ttggcaccat
    41341 tgaacatcgc gtcaaggggc tggagttggg ggccgatgac tatctggtga agccgttcgc
    41401 gtttgctgaa ctgctggcgc gggtgcgcac tctcctgcgg cgcggggcgg cggtgattat
    41461 cgaaagtcag tttcaggttg ccgatttgat ggtcgatctc gtcagccgca aagtcacccg
    41521 cagcggcacg cgcatcactc tgaccagtaa agagtttact ctgctggagt tttttctccg
    41581 ccatcagggc gaagtgctgc cccgctcgct tatcgcctcg caggtatggg acatgaattt
    41641 tgacagcgac actaacgcca ttgatgtggc ggtgaagcgg ctacgcggca aaatcgacaa
    41701 cgactttgag ccgaagctga ttcaaaccgt gcgcggcgtg ggttacatgc ttgaggtgcc
    41761 ggatggtcag taagccattt cagcgcccgt tttcgctggc aacccgcctg acctttttta
    41821 tcagcctggc caccatcgcg gcgtttttcg cctttgcatg gatcatgatc cactcagtaa
    41881 aagtgcattt tgccgagcag gatattaatg atttaaaaga gattagtgcc acccttgaac
    41941 gcgtactaaa tcaccctgac gaaacgcaag cccgacgctt aatgacgctg gaagatatcg
    42001 tcagtggtta ttccaatgtg ttgatttccc tggcagatag ccacggtaaa acggtgtatc
    42061 actcccccgg tgcgcctgat atccgcgagt ttgcgcgcga tgccataccc gataaagacg
    42121 cgcggggcgg cgaggtgttt ctcctctccg gcccgacgat gatgatgcca ggccacggtc
    42181 acgggcatat ggaacacagc aactggcgga tgatcagttt gccagttggc ccactggttg
    42241 acggcaaacc gatttatacg ctctacatcg cgctttcgat cgattttcat cttcattaca
    42301 taaatgattt gatgaataaa cttattatga ccgcatcggt catcagcatc ctgatcgtct
    42361 ttatcgtgct attggcggtt cataaaggtc acgcgccgat ccgcagcgtc agccgtcaaa
    42421 tccagaatat cacctcgaaa gatctcgacg tccgccttga cccgcagacc gtgccgattg
    42481 agctggaaca gctggtactg tcgttcaacc atatgatcga gcgtattgag gatgtcttta
    42541 cccgccagtc caatttctca gcggatatcg cccacgaaat tcgcacacca attactaatc
    42601 tcataacgca aacggaaatc gccctcagcc agtcgcgcag ccagaaggag ctggaagatg
    42661 tgctctactc taatctcgaa gagctgacgc gaatggcgaa aatggtcagc gatatgctgt
    42721 ttctcgccca ggccgataac aaccagttaa tccccgaaaa gaaaatgctc aacctggcgg
    42781 atgaagtcgg caaagtgttc gattttttcg aggctttagc ggaagatcgc ggcgtggagc
    42841 tgcagtttgt tggcgacgag tgccaggttg cgggcgatcc gctgatgctg cgtcgggcgt
    42901 taagcaacct gctttcgaac gccctgcgtt atacgccacc cggagaggct attgtagtgc
    42961 gctgccagac ggtcgatcat ctggtgcaag ttatcgtcga aaaccccggt acgcccattg
    43021 cgcccgagca cttaccgcga ttgtttgacc gtttctaccg cgttgacccc tcccgccaac
    43081 gaaaaggcga aggtagcggc attgggctgg cgatagtgaa atcaattgtt gtcgcgcata
    43141 aaggcacggt tgcggttacg tcaaatgcgc gggggacaag gtttgtgatt gtgttgccgg
    43201 agagggggtg attattaagt tcaaattcag ggcgagggtt tacggcgttt ttcgctaaat
    43261 ttccatcatt ataaac
//
LOCUS       DAZFDC010000020        42665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFDC010000020 DAZFDC010000000
VERSION     DAZFDC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.020"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            121..1689
                     /gene="cydA"
                     /locus_tag="ACHJ48_001279"
     CDS             121..1689
                     /gene="cydA"
                     /locus_tag="ACHJ48_001279"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO4706878.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1705..2844
                     /gene="cydB"
                     /locus_tag="ACHJ48_001280"
     CDS             1705..2844
                     /gene="cydB"
                     /locus_tag="ACHJ48_001280"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4706879.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2859..2972
                     /gene="cydX"
                     /locus_tag="ACHJ48_001281"
     CDS             2859..2972
                     /gene="cydX"
                     /locus_tag="ACHJ48_001281"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO4706880.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            2972..3265
                     /gene="ybgE"
                     /locus_tag="ACHJ48_001282"
     CDS             2972..3265
                     /gene="ybgE"
                     /locus_tag="ACHJ48_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO4706881.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3415..3819
                     /gene="ybgC"
                     /locus_tag="ACHJ48_001283"
     CDS             3415..3819
                     /gene="ybgC"
                     /locus_tag="ACHJ48_001283"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4706882.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3816..4508
                     /gene="tolQ"
                     /locus_tag="ACHJ48_001284"
     CDS             3816..4508
                     /gene="tolQ"
                     /locus_tag="ACHJ48_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO4706883.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4512..4940
                     /gene="tolR"
                     /locus_tag="ACHJ48_001285"
     CDS             4512..4940
                     /gene="tolR"
                     /locus_tag="ACHJ48_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO4706884.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5005..6219
                     /gene="tolA"
                     /locus_tag="ACHJ48_001286"
     CDS             5005..6219
                     /gene="tolA"
                     /locus_tag="ACHJ48_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO4706885.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADDKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAE
                     ADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESK
                     FYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVY
                     EVFKNAPLDFKP"
     gene            6352..7644
                     /gene="tolB"
                     /locus_tag="ACHJ48_001287"
     CDS             6352..7644
                     /gene="tolB"
                     /locus_tag="ACHJ48_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO4706886.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7679..8200
                     /gene="pal"
                     /locus_tag="ACHJ48_001288"
     CDS             7679..8200
                     /gene="pal"
                     /locus_tag="ACHJ48_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO4706887.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8210..9001
                     /gene="cpoB"
                     /locus_tag="ACHJ48_001289"
     CDS             8210..9001
                     /gene="cpoB"
                     /locus_tag="ACHJ48_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO4706888.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVIQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9166..9241
                     /locus_tag="ACHJ48_001290"
     tRNA            9166..9241
                     /locus_tag="ACHJ48_001290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9199..9201,aa:Lys,seq:ttt)
     gene            9277..9352
                     /locus_tag="ACHJ48_001291"
     tRNA            9277..9352
                     /locus_tag="ACHJ48_001291"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9310..9312,aa:Val,seq:tac)
     gene            9355..9430
                     /locus_tag="ACHJ48_001292"
     tRNA            9355..9430
                     /locus_tag="ACHJ48_001292"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9388..9390,aa:Lys,seq:ttt)
     gene            9482..9557
                     /locus_tag="ACHJ48_001293"
     tRNA            9482..9557
                     /locus_tag="ACHJ48_001293"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9515..9517,aa:Val,seq:tac)
     gene            9561..9636
                     /locus_tag="ACHJ48_001294"
     tRNA            9561..9636
                     /locus_tag="ACHJ48_001294"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9594..9596,aa:Lys,seq:ttt)
     gene            9783..9858
                     /locus_tag="ACHJ48_001295"
     tRNA            9783..9858
                     /locus_tag="ACHJ48_001295"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9816..9818,aa:Lys,seq:ttt)
     gene            10136..11179
                     /gene="nadA"
                     /locus_tag="ACHJ48_001296"
     CDS             10136..11179
                     /gene="nadA"
                     /locus_tag="ACHJ48_001296"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO4706889.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKKTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNYEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11217..11936
                     /gene="pnuC"
                     /locus_tag="ACHJ48_001297"
     CDS             11217..11936
                     /gene="pnuC"
                     /locus_tag="ACHJ48_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO4706890.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11933..12874)
                     /gene="zitB"
                     /locus_tag="ACHJ48_001298"
     CDS             complement(11933..12874)
                     /gene="zitB"
                     /locus_tag="ACHJ48_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO4706891.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(12988..13368)
                     /gene="ybgS"
                     /locus_tag="ACHJ48_001299"
     CDS             complement(12988..13368)
                     /gene="ybgS"
                     /locus_tag="ACHJ48_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO4706892.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13684..14736
                     /gene="aroG"
                     /locus_tag="ACHJ48_001300"
     CDS             13684..14736
                     /gene="aroG"
                     /locus_tag="ACHJ48_001300"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO4706893.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(14902..15654)
                     /gene="gpmA"
                     /locus_tag="ACHJ48_001301"
     CDS             complement(14902..15654)
                     /gene="gpmA"
                     /locus_tag="ACHJ48_001301"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO4706894.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(15856..16896)
                     /gene="galM"
                     /locus_tag="ACHJ48_001302"
     CDS             complement(15856..16896)
                     /gene="galM"
                     /locus_tag="ACHJ48_001302"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO4706895.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGKQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            complement(16890..18038)
                     /gene="galK"
                     /locus_tag="ACHJ48_001303"
     CDS             complement(16890..18038)
                     /gene="galK"
                     /locus_tag="ACHJ48_001303"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO4706896.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18042..19088)
                     /gene="galT"
                     /locus_tag="ACHJ48_001304"
     CDS             complement(18042..19088)
                     /gene="galT"
                     /locus_tag="ACHJ48_001304"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO4706897.1"
                     /translation="MTQFNPVDHPHRRYNQLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19098..20114)
                     /gene="galE"
                     /locus_tag="ACHJ48_001305"
     CDS             complement(19098..20114)
                     /gene="galE"
                     /locus_tag="ACHJ48_001305"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO4706898.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20376..21848)
                     /gene="modF"
                     /locus_tag="ACHJ48_001306"
     CDS             complement(20376..21848)
                     /gene="modF"
                     /locus_tag="ACHJ48_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO4706899.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITPLLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYHYALTKIN"
     gene            complement(21916..22704)
                     /gene="modE"
                     /locus_tag="ACHJ48_001307"
     CDS             complement(21916..22704)
                     /gene="modE"
                     /locus_tag="ACHJ48_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO4706900.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            22833..22982
                     /gene="acrZ"
                     /locus_tag="ACHJ48_001308"
     CDS             22833..22982
                     /gene="acrZ"
                     /locus_tag="ACHJ48_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO4706901.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23149..23922
                     /gene="modA"
                     /locus_tag="ACHJ48_001309"
     CDS             23149..23922
                     /gene="modA"
                     /locus_tag="ACHJ48_001309"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4706902.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            23922..24611
                     /gene="modB"
                     /locus_tag="ACHJ48_001310"
     CDS             23922..24611
                     /gene="modB"
                     /locus_tag="ACHJ48_001310"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO4706903.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24614..25672
                     /gene="modC"
                     /locus_tag="ACHJ48_001311"
     CDS             24614..25672
                     /gene="modC"
                     /locus_tag="ACHJ48_001311"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO4706904.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25673..26491)
                     /gene="ybhA"
                     /locus_tag="ACHJ48_001312"
     CDS             complement(25673..26491)
                     /gene="ybhA"
                     /locus_tag="ACHJ48_001312"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO4706905.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDEMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26646..27641
                     /gene="pgl"
                     /locus_tag="ACHJ48_001313"
     CDS             26646..27641
                     /gene="pgl"
                     /locus_tag="ACHJ48_001313"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO4706906.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(27682..28635)
                     /gene="ybhD"
                     /locus_tag="ACHJ48_001314"
     CDS             complement(27682..28635)
                     /gene="ybhD"
                     /locus_tag="ACHJ48_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4706907.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            28819..29871
                     /gene="ybhH"
                     /locus_tag="ACHJ48_001315"
     CDS             28819..29871
                     /gene="ybhH"
                     /locus_tag="ACHJ48_001315"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO4706908.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEKRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGGLDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            29947..31380
                     /gene="ybhI"
                     /locus_tag="ACHJ48_001316"
     CDS             29947..31380
                     /gene="ybhI"
                     /locus_tag="ACHJ48_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO4706909.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESSVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            31563..33824
                     /gene="ybhJ"
                     /locus_tag="ACHJ48_001317"
     CDS             31563..33824
                     /gene="ybhJ"
                     /locus_tag="ACHJ48_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO4706910.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGAAQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGSSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     VEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(33965..35248)
                     /gene="ybhC"
                     /locus_tag="ACHJ48_001318"
     CDS             complement(33965..35248)
                     /gene="ybhC"
                     /locus_tag="ACHJ48_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO4706911.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35383..36449)
                     /locus_tag="ACHJ48_001319"
                     /pseudo
     CDS             complement(35383..36449)
                     /locus_tag="ACHJ48_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(36427..36645)
                     /locus_tag="ACHJ48_001320"
     CDS             complement(36427..36645)
                     /locus_tag="ACHJ48_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4706912.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(36685..36852)
                     /locus_tag="ACHJ48_001321"
     CDS             complement(36685..36852)
                     /locus_tag="ACHJ48_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706913.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
     gene            37095..37697
                     /locus_tag="ACHJ48_001322"
     CDS             37095..37697
                     /locus_tag="ACHJ48_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706914.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVLGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            complement(37908..38129)
                     /locus_tag="ACHJ48_001323"
     CDS             complement(37908..38129)
                     /locus_tag="ACHJ48_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4706915.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(38228..38443)
                     /locus_tag="ACHJ48_001324"
     CDS             complement(38228..38443)
                     /locus_tag="ACHJ48_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4706916.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQPFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(38520..38711)
                     /locus_tag="ACHJ48_001325"
     CDS             complement(38520..38711)
                     /locus_tag="ACHJ48_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4706917.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDLV"
     gene            complement(38684..38866)
                     /locus_tag="ACHJ48_001326"
     CDS             complement(38684..38866)
                     /locus_tag="ACHJ48_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4706918.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKREAICTKHLLLS"
     gene            complement(38863..39543)
                     /locus_tag="ACHJ48_001327"
     CDS             complement(38863..39543)
                     /locus_tag="ACHJ48_001327"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4706919.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(39540..40211)
                     /gene="bet"
                     /locus_tag="ACHJ48_001328"
                     /pseudo
     CDS             complement(39540..40211)
                     /gene="bet"
                     /locus_tag="ACHJ48_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            40241..40423
                     /locus_tag="ACHJ48_001329"
     CDS             40241..40423
                     /locus_tag="ACHJ48_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4706920.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            40420..40590
                     /locus_tag="ACHJ48_001330"
     CDS             40420..40590
                     /locus_tag="ACHJ48_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO4706921.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEKDDG"
     gene            40583..41203
                     /locus_tag="ACHJ48_001331"
     CDS             40583..41203
                     /locus_tag="ACHJ48_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4706922.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVHAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            41200..41865
                     /locus_tag="ACHJ48_001332"
     CDS             41200..41865
                     /locus_tag="ACHJ48_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO4706923.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            41862..42485
                     /locus_tag="ACHJ48_001333"
     CDS             41862..42485
                     /locus_tag="ACHJ48_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4706924.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
BASE COUNT        10358 a        10365 c        11069 g        10873 t
ORIGIN      
        1 cctcttttga tgaaatggca acgatagagg cgagcaaata attttgtatt ggggcatgcg
       61 tgtgaccctt tctaacgggg ttcactctcg gagtcttcat gcgatgagca aggagtcatg
      121 atgttagata tagtcgaact gtcgcgctta cagtttgcct tgaccgcgat gtaccacttc
      181 ctttttgtgc cactgacgct cggtatggcg ttcctgctgg ccattatgga aacggtctac
      241 gtcctttccg gcaaacagat ttataaagat atgaccaagt tctggggcaa gttgtttggt
      301 atcaacttcg ctctgggtgt ggctaccggt ctgaccatgg agttccagtt cgggactaac
      361 tggtcttact attcccacta tgtaggggat atcttcggtg cgccgctggc aatcgaaggt
      421 ctgatggcct tcttcctcga atccaccttt gtaggtctgt tcttcttcgg ttgggatcgt
      481 ctgggtaaag ttcagcatat gtgtgtcacc tggctggtgg cgctcggttc aaacctgtcc
      541 gcactgtgga ttctggttgc gaacggctgg atgcaaaacc caatcgcgtc cgatttcaac
      601 tttgaaacta tgcgtatgga gatggtgagc ttctccgagc tggtgcttaa cccggttgct
      661 caggtgaaat tcgttcacac tgtagcgtct ggttatgtga ctggcgcgat gttcatcctc
      721 ggtatcagcg catggtatat gctgaaaggt cgtgacttcg ccttcgctaa acgctccttt
      781 gctatcgctg ccagcttcgg tatggctgcc gttctgtctg ttattgttct gggtgatgaa
      841 tccggctacg aaatgggcga cgtgcagaaa accaaactgg ctgctattga agctgagtgg
      901 gaaacgcaac ctgcgcctgc tgcctttact ctgttcggca ttcctgatca ggaagaggag
      961 acgaacaaat ttgcgatcca gatcccttac gcgctgggca taattgcaac gcgttccgtg
     1021 gatactccag ttatcggcct gaaagaactg atggtgcagc atgaagaacg cattcgtaac
     1081 gggatgaagg cgtactctct gctcgaacaa ctgcgttctg gttctaccga ccaggcggtt
     1141 cgtgaccagt tcaatagcat gaagaaggac ctcggttacg gtctgctgct gaaacgctat
     1201 acgccaaacg tggctgatgc gactgaagcg cagattcaac aggcaaccaa agactccatt
     1261 ccgcgtgtag cgccgctgta cttcgcgttc cgtatcatgg tggcgtgtgg cttcctgctg
     1321 ctggcaatca tcgcgctctc tttctggagt gtcatccgca accgcattgg cgagaaaaaa
     1381 tggctcctgc gcgccgcgct gtacggtatt ccgctgccgt ggattgctgt agaagcgggc
     1441 tggttcgtgg ctgaatatgg ccgccaaccg tgggctatcg gtgaagtgct gccgacagct
     1501 gtggcgaact cgtcactgac cgcaggcgat ctcatcttct caatggtgct gatttgcggc
     1561 ctgtataccc tgttcctggt ggcagaattg ttcttaatgt tcaagtttgc acgcctcggc
     1621 ccaagcagcc tgaaaaccgg tcgctatcac tttgagcagt cttccacgac tactcagccg
     1681 gcacgctaag acaggagtcg tcaaatgatc gattatgaag tattgcgttt tatctggtgg
     1741 ctgctggttg gcgttctgct gattggtttt gcagtcactg atggtttcga catgggggtg
     1801 ggcatgctca cccgtttcct cggtcgtaac gacaccgagc gtcgaattat gattaactcc
     1861 atcgcaccac actgggacgg taaccaggtt tggctgatca ccgcaggcgg cgcactgttt
     1921 gctgcctggc cgatggtcta tgccgctgcg ttctccggct tctatgtggc gatgatcctc
     1981 gtgctggcgt ctttgttctt ccgtccggtc ggttttgact accgctccaa gattgaagaa
     2041 acccgctggc gtaacatgtg ggactggggc atcttcattg gtagcttcgt tccgccgttg
     2101 gtgattggtg tagcgttcgg taacctgttg cagggcgtac cgttcaacgt tgatgaatat
     2161 ctgcgtctgt actacaccgg taacttcttc cagctgctta acccgttcgg cctgctggca
     2221 ggcgtggtga gcgtagggat gatcattact cagggcgcaa cctatctgca aatgcgtacc
     2281 gtgggcgaac tgcacctgcg tacccgtgca acggctcagg tggcggcact ggtgacactg
     2341 gtctgtttcg cactggctgg cgtatgggtg atgtacggta tcgatggtta tgtcgtgaaa
     2401 tcgacaatgg accattacgc agcctctaac ccgctgaata aagaagtggt tcgtgaagct
     2461 ggcgcatggc tggttaactt caacaacacg ccaattctgt gggctattcc ggcactgggt
     2521 gtggttctgc cgctgctgac catcctgact gcacgtatgg ataaagccgc gtgggcgttt
     2581 gtgttctcct ccctgacgct ggcctgcatc atcctgactg ccggtatcgc aatgttcccg
     2641 tttgtgatgc cgtccagcac catgatgaac gcaagtctga caatgtggga tgcaacttcc
     2701 agccagctga cacttaacgt catgacctgg gttgcggtgg ttctggtacc gatcattctg
     2761 ctctacaccg cctggtgtta ctggaaaatg ttcggtcgta tcaccaaaga agatattgaa
     2821 cgtaacaccc actctctgta ctaagtaagg agctaaaaat gtggtatttc gcatggattc
     2881 tgggaacgct tcttgcctgt tcgtttgggg taatcaccgc gctggcgctt gagcacgtcg
     2941 aatcaggcaa agccggtcaa gaagacatct gatgagtaag attatcgcaa ccttgtatgc
     3001 ggtaatggac aagcgccccc tgcgggcgct ttccttcgtg atggcgcttc tgttagcagg
     3061 atgtatgttt tgggacccat ctcgtttcgc cgcgaagacc agtgaactgg aaatctggca
     3121 tggtttattg ctgatgtggg ccgtctgtgc tggtgtgatt cacggcgtgg gctttcgtcc
     3181 gcagaaggtt ctttggcaag ggattttttg cccattgctt gccgatattg ttctcattgt
     3241 cgggctgatt ttcttcttct tttaaatcag aattctcttt aaaaaattat gggccgtgcc
     3301 aggcccataa atttttacgc tcccttaact tgccctcatt cccaaacctc aatcgcgcgc
     3361 gtatagtagc agcgtttaaa agctctaact tttgttgcat taccgggatg taaagtgaat
     3421 acaacgctgt ttcgatggcc ggttcgcgtc tactatgaag ataccgatgc cggtggtgtg
     3481 gtgtaccacg ccagttacgt cgctttttat gaaagagcac gcacagagat gctgcgtcat
     3541 catcacttca gtcaacaggc gctgatggct gaacgcgttg cctttgtggt acgtaaaatg
     3601 acggtggaat attacgcacc tgcgcggctc gacgatatgc tcgaaataca gactgaaata
     3661 acatcaatgc gtggcacctc tttggttttc acgcaacgta ttgtcaacgc cgagaatact
     3721 ttgctgaatg aagcagaggt tctggttgtt tgcgttgacc cactcaaaat gaagcctcgt
     3781 gcgcttccca agtctattgt cgcggagttt aagcagtgac tgacatgaat atccttgatt
     3841 tgttcctgaa ggctagcctt ctggttaaac ttatcatgtt gattttgatt ggtttttcaa
     3901 tcgcatcctg ggccattatt atccagcgga cccgtattct taacgcagcg gcgcgcgaag
     3961 ccgaagcgtt tgaagataaa ttctggtctg gaatcgaact ctctcgcctc tatcaagaga
     4021 gccaggggaa acgggataat ctgactggtt cggaacaaat cttttacagc gggttcaaag
     4081 aatttgtgcg cctgcatcgt gccaatagcc atgcgccgga agccgtagtg gaaggggcgt
     4141 cgcgtgcgat gcgtatttcc atgaaccgcg aacttgaaaa tctggaaacg cacattccgt
     4201 tcctcggtac ggttggctcc atcagcccgt atattggtct gtttggtacg gtctggggga
     4261 tcatgcacgc ctttatcgcc ctcggggcgg taaaacaagc aacactgcaa atggttgcgc
     4321 ccggtatcgc agaagcgttg attgcgacgg caattggtct gttcgccgct attccggcgg
     4381 taatggctta taaccgtctc aaccaacgtg taaacaaact ggaactgaat tacgacaact
     4441 ttatggaaga gtttaccgcg attctgcacc gccaggcgtt taccgttagc gagagcaaca
     4501 aggggtaagc catggccaga gcgcgtggac gaggtcgtcg cgatctcaag tccgaaatca
     4561 acattgtacc gttgctggac gtactgctgg tgctgttgct gatctttatg gcgacagcgc
     4621 ccatcatcac ccagagcgtg gaggtcgatt tgccagacgc tactgaatca caggcggtga
     4681 gcagtaacga taatccgcca gtgattgttg aagtgtctgg tattggtcag tacaccgtgg
     4741 tggttgagaa agatcgcctg gagcgtttac caccagagca ggtggtggcg gaagtgtcca
     4801 gccgtttcaa ggccaacccg aaaacggtct ttctgatcgg tggcgcaaaa gatgtgcctt
     4861 acgatgaaat aattaaagca ctgaacttgt tacatagtgc gggtgtgaaa tcggttggtt
     4921 taatgacgca gcctatctaa acatctgcgt ttcccttgct tgaaagagag cgggtaacag
     4981 gcgaacagtt tttggaaacc gagagtgtca aaggcaaccg aacaaaacga caagctcaag
     5041 cgggcgataa ttatttcagc agtgctgcat gtcatcttat ttgcggcgct gatctggagt
     5101 tcgttcgatg agaatataga agcttcagct ggaggcggcg gtggttcgtc catcgacgct
     5161 gtcatggttg attcaggtgc ggtagttgag cagtacaaac gcatgcaaag ccaggaatca
     5221 agcgcgaagc gttctgatga gcagcgcaag atgaaggaac agcaggctgc tgaagaactg
     5281 cgtgagaaac aagcggctga acaggaacgc ctgaagcaac ttgagaaaga gcggttagct
     5341 gctcaggaac agaaaaagca ggctgaagaa gccgcaaaac aggccgagtt aaagcagaag
     5401 caagcggaag aggcggcagc gaaagcggcg gcagatgcta aagcgaaggc cgaagcggat
     5461 gataaagctg cggaagaagc agcgaagaaa gcggctgcag acgcgaagaa aaaagcagaa
     5521 gcagaagccg ccaaagccgc agccgaagcg cagaaaaaag ccgaggcagc agctgcggcg
     5581 ctgaagaaga aagcggaagc ggcagaagca gctgcagctg aagcaagaaa gaaagcggca
     5641 gcagagaaag ctgcagccga caaaaaagca gcagagaaag ctgcagccga caaaaaagca
     5701 gcagaaaaag cggctgctga aaaggcagca gctgataaga aagcagcggc agaaaaagcc
     5761 gccgcagaca aaaaagcggc agctgcaaaa gcagcagctg aaaaagccgc tgcagcaaaa
     5821 gctgccgcgg aggcagatga tattttcggt gagctaagct ctggtaagaa tgcaccgaaa
     5881 acggggggag gggcgaaagg gaacaatgct tcgcctgccg ggagtggtaa tactaaaaac
     5941 aatggcgcat caggggccga tatcaataac tatgccgggc agattaaatc tgctatcgaa
     6001 agtaagttct atgacgcatc gtcctatgca ggcaaaacct gtacgctgcg cataaaactg
     6061 gcacccgatg gtatgttact ggatatcaaa cctgaaggtg gcgatcccgc actttgtcag
     6121 gctgcgttgg cagcagctaa acttgcgaag atcccgaaac caccaagcca ggcagtatat
     6181 gaagtgttca aaaacgcgcc attggacttc aaaccgtaat cgcgatgttg actgttccga
     6241 cggtcaacat caggcaccgg ttgccacggg gttctggtag ttttgtgtat tttagtttgt
     6301 taacattctg ctaaattatc gtgggccatc ggtccagata agggagatat gatgaagcag
     6361 gcattacgag tagcatttgg ttttctcata ctgtgggcat cagttctgca tgctgaagtc
     6421 cgcattgtga tcgacagcgg tgtagattcc ggtcgtccta ttggagttgt tcctttccag
     6481 tgggcgggcc ctggtgcggc acctgaagat attggcggca tcgttgctgc cgacttgcgt
     6541 aacagcggta aatttaatcc gttagatcgc gctcgtctgc cacagcagcc gggtagtgcg
     6601 caggaagtac aaccagctgc atggtccgcg ctgggtattg acgctgtagt tgtcggtcag
     6661 gtcactccga atccggatgg ttcttacaat gttgcttatc aacttgttga cactggcggc
     6721 gcaccgggta ctgtacttgc tcagaactcg tacaaagtga acaagcagtg gctgcgttat
     6781 gctggtcata ccgccagtga tgaagtgttt gaaaaactga ccggtattaa aggtgcgttc
     6841 cgtacccgta ttgcctacgt tgttcagacc aacggcggtc agttcccgta tgaactgcgc
     6901 gtatctgact atgacggtta caaccagttt gtcgttcacc gttcaccaca gccgctgatg
     6961 tccccggcgt ggtcaccaga cggttctaaa ctggcttatg tgaccttcga aagcggtcgt
     7021 tccgcgctgg ttatacaaac gctggcaaac ggcgctgtac gtcaggtggc ttcattcccg
     7081 cgtcacaacg gtgcgcctgc attctcgcca gacggcagca aactggcatt cgccttgtcg
     7141 aaaaccggta gcctgaacct gtacgtaatg gatttggctt ctggtcagat ccgccaggtg
     7201 actgatggtc gcagtaacaa taccgaaccg acctggttcc cggacagcca gaacctggca
     7261 tttacttctg accaggccgg tcgtccacag gtttataaag tgaatatcaa cggcggtgcg
     7321 ccacaacgta ttacctggga aggttcgcag aaccaggatg cggatgtcag cagcgacggt
     7381 aaatttatgg taatggtcag ctccaatggt gggcagcagc acattgccaa acaagatctg
     7441 gcaacgggag gcgtacaagt tctgtcgtcc acgttcctgg atgaaacgcc aagtctggca
     7501 cctaacggca ctatggtaat ctacagctct tctcagggga tgggatccgt gctgaatttg
     7561 gtttctacag atgggcgttt caaagcgcgt cttccggcaa ctgatggaca ggtcaaattc
     7621 cctgcctggt cgccgtatct gtgataataa ttaattgaat agtaaaggaa tcattgaaat
     7681 gcaactgaac aaagtgctga aagggctgat gattgctctg cctgttatgg caattgcggc
     7741 atgttcttcc aacaagaacg ccagcaatga cggcagcgaa ggcatgctgg gtgccggcac
     7801 tggtatggat gcgaacggcg gcaacggcaa catgtcttcc gaagagcagg ctcgtctgca
     7861 aatgcaacag ctgcagcaga acaacatcgt ttacttcgat ctggacaagt acgatatccg
     7921 ttctgacttc gctcaaatgc tggatgcaca tgcaaacttc ctgcgtagca acccgtctta
     7981 caaagtcacc gtagaaggtc acgcggacga acgtggtact ccggaataca acatctccct
     8041 gggtgaacgt cgtgcgaacg ccgttaagat gtacctgcag ggtaaaggcg tttctgcaga
     8101 ccagatctcc atcgtttctt acggtaaaga aaaacctgca gtactgggtc atgacgaagc
     8161 ggcatactcc aaaaaccgtc gtgcggtact ggtttactaa gagaattgca tgagcagtaa
     8221 cttcagacat caactattga gtctgtcgtt actggttggt atagcggccc cctgggccgc
     8281 ttttgctcag gcaccaatca gtagtgtcgg ctcaggctcg gtcgaagacc gcgtcattca
     8341 acttgagcgt atttctaatg ctcacagcca gcttttaacc caactccagc aacaactctc
     8401 tgataatcaa tccgatattg attccctgcg tggtcagatt caggaaaatc agtatcaact
     8461 gaatcaggtc gtggagcggc aaaagcagat cctgttgcag atcgacagcc tcagcagcgg
     8521 tggtgcagcg gcgcaatcaa ccagcggtga tcaaagcggt gcggcggcat caacgacgcc
     8581 gacagctgat gctggtactg cgaatgctgg cgcgccggtg aaaagcggtg atgcaaacac
     8641 ggattacaat gcagctattg cgctggtgca ggataaatcc cgccaggatg acgcaatggt
     8701 ggcatttcag aatttcatca aaaattaccc tgattcaact tacctgccaa acgccaatta
     8761 ttggctgggt cagttaaact acaacaaggg taaaaaagat gatgcggcgt actattttgc
     8821 ttcggtagtg aaaaactatc cgaagtcacc aaaggctgca gatgcgatgt ttaaagtcgg
     8881 cgtcatcatg caggacaaag gtgacaccgc aaaagcgaaa gccgtatacc agcaggttat
     8941 cagtaaatac cctggtactg atggcgctaa acaggcacaa aaacgtctga acgcgatgta
     9001 atgcataaca cacgaccaga agtcgcatta tttctggtcg tgtcgtgcga atcataagca
     9061 gttgagtgat ctacatcgaa atttttgttg cgctcaagtc tgaaatcagt aatatatgcc
     9121 gccgttgcca cgggatatca aacaaaccga aagcaacgaa aaagtgggtc gttagctcag
     9181 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
     9241 actaacctag ttagttgtag tatccagcgt aatatcgggt gattagctca gctgggagag
     9301 cacctccctt acaaggaggg gggcggcggt tcgatcccgt catcacccac caccgggtcg
     9361 ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca
     9421 cgacccacca ctaattacgg tgggttcggt ggaagtagtt tgtagtatcc agcgcagtat
     9481 cgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg gcggttcgat
     9541 cccgtcatca cccaccactc gggtcgttag ctcagttggt agagcagttg acttttaatc
     9601 aattggtcgc aggttcgaat cctgcacgac ccaccaattt taacatcaaa ctcagatgtt
     9661 aagcgtgaag gataacgttg cgtcagcaac ggcccgtagg gcaagcgaag cgagtcatcc
     9721 tgcacgaccc accaatttta aagattggtt ccgagtagaa atctttcagg taacacccgt
     9781 atgggtcgtt agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga
     9841 atcctgcacg acccaccaat gtaaaaaagc gccctaaagg cgcttttttg ctattcaggc
     9901 atctctcaat ttcaattctt gaccctgatg acatactccg cgcttgctgc gtaaacaact
     9961 ttacgtcgga ggaataagac gcaaagtgtg gcattcggca tctcatacgt aattcgagtg
    10021 acttttcccc accattcgac tatcttgttt agcatataaa acaaattaca ccgataacag
    10081 cgaatatcac gctaatgtcg gttttaacgt taagcctgta aaacgagatg gtaagatgag
    10141 cgtgatgttt gatccagaca cggcgattta tcctttcccc ccgaagccga cgccgttaag
    10201 cattgatgaa aaagcgtatt accgcgagaa gataaaacgt ctgctaaaag aacgtaatgc
    10261 ggtgatggtt gcccactact ataccgatcc cgaaattcaa caactggcag aagaaaccgg
    10321 tggctgtatt tctgattctc tggaaatggc gcgcttcggt gcaaagcatc ccgcttctac
    10381 gttgttagtc gctggggtga gatttatggg agaaaccgcc aaaattctca gtccggaaaa
    10441 aacaattctg atgccgacac ttcaggctga gtgttcactg gatctcggct gccctgttga
    10501 agaatttaac gcattttgcg atgcccatcc cgatcgtact gtcgtcgtct acgccaacac
    10561 ttctgctgcg gtaaaagcgc gggcagattg ggtggtaact tcaagcattg ccgtcgaact
    10621 tattgatcat ctggatagtt tgggtgaaaa aatcatctgg gcacccgaca aacatctggg
    10681 gcgttacgtg caaaaaaaga cgggcgcaga cattttatgc tggcagggtg cctgcattgt
    10741 gcatgatgag tttaagactc aggcgttaac ccgcttgcaa gaagaatacc cggatgctgc
    10801 catactggtg catccagaat caccacaagc tattgtcgat atggcggatg cggtcggttc
    10861 caccagtcaa ctgatcgctg ctgcgaaaac attgccacat cagaggctta ttgtggcaac
    10921 cgatcggggt attttctaca aaatgcagca ggcggtgcca gataaagagt tactggaagc
    10981 accaaccgcg ggtgagggcg caacctgccg cagctgcgcg cattgtccgt ggatggccat
    11041 gaatggcctc caggccatcg ctgaggcatt agaacaggaa ggaagcaatt acgaggttca
    11101 tgttgatgaa aggctgcgag agagggcgct ggtgccgctc aatcgtatgc tggattttgc
    11161 ggctacacta cgtggataac gaataataag gcgtaacgtt acgctttggg ggaaagatgg
    11221 atttttttag tgtgcagaat atcctggtac atataccaat aggagcaggc ggttatgatc
    11281 tctcatggat cgaagcggta ggcacaatcg ccgggttgct gtgtattggc cttgccagtc
    11341 tggagaagat cagcaactac ttctttggcc tgatcaacgt caccttgttt ggcattattt
    11401 tctttcagat tcagctgtat gccagcctgc tattacaggt gtttttcttt gccgcgaata
    11461 tttacggctg gtatgcgtgg tcgcgacaaa ccagtcagaa cgaggcggag ttaaaaattc
    11521 gctggttgcc attgccgaag gcactcagct ggttggcggt ttgcgttgtt tcgattggtc
    11581 tgatgacggt atttatcaat ccggtgtttg catttttgac ccgcgtggca gtcatcatca
    11641 tgcaagcatt aggattacag gttgcgatgc ctgaactaca accggacgct ttcccgttct
    11701 gggattcttg catgatggtg ttatctatcg tggcaatgat tctgatgact cgtaagtatg
    11761 tggaaaactg gctgttgtgg gtgattatta acgtgattag cgtcgttatt tttgcacttc
    11821 agggcgttta cgccatgtct ctggagtaca tcatcctgac ctttattgcg ctcaacggca
    11881 gccggatgtg gatcaatagc gcacgcgaaa gaggctcacg cgcgctgtcc cattaatggt
    11941 gatgatgtga atggccagac accccttcat tcaggtggca gtctggccca tgacaaggtt
    12001 gatattccat ctgaatggtg gcgtgctcaa tctgatagtg atccatcagg tagtgttgga
    12061 tctgatccaa caaggcatcg tgatcgtgtg gcgggatcac ctgcacatgc aacgtcatca
    12121 ccggcttctc gcctaccatc catacatgca catggtgcac attgcggact tccgggattt
    12181 cccggcacat acgacgcttc agttcagcga tatccagcga taccggtgca ccctcaagta
    12241 attcattcac gctatctttc aacagtcgcc acgcgctacg cagaaccaga agcgacacca
    12301 gtatcgagag aatggggtca gcaggtgtcc agccggtcca gataataatc aacgcggcaa
    12361 taatagcccc aaccgaaccc agcaggtccc cgagcacatg cagtgccgct gctcgtacgt
    12421 tgaggttttt ctcttcgctg ccgtgatgaa gtaaccagaa agaaagtata tttgccagca
    12481 acccagccac ggcaattgcc atcatcatgc cgccctcgac cggacgcggc gtgcggaagc
    12541 gttctatcgc ctcccagaca atcaaaatgg taattaccac gagggcgatg gcattcacaa
    12601 acgccgcgag cgtggtcaat cttagccaac cgaaagtgtg gcgaatggtg ggaggacgac
    12661 gtgaaaattg cacggcgagt agggcgaaaa gcagggcggc agtatcggtc aacatatgac
    12721 ccgcatcggc cagcaaagcc agagaaccgg aaagaaaacc accaatgact tctaccagca
    12781 taaacccggc agtcacgccg aaagcataca acaagcgacg agcattatta tcttctggca
    12841 ggtgtgagga cgtgtgtgag tgtgagtgcg ccatgacgag tcatccctta attattctca
    12901 cttaacttta tgacatcata ctgcttttag aagggaaaaa ttaaaaggga gagactccgc
    12961 tctcccatta ttggctattt tgcagggtta ctgcgtggta ccgtcggttt tggtatcgac
    13021 atcattattg atgccatcac cggtttgtac ctttttattg atatccgggc agcgaccatc
    13081 tttgcacatg gtgttcttgt gctcttcatc tttggtcatg ccgtcattgt tcattgaaga
    13141 accatccgga tgcagcatag tgccgccaga accggtattt accccgttat tgtcgacgtt
    13201 atttggcgcg acattttcac gggcgtcagg ggctacctgg cccgcatcag ctgcggcgtt
    13261 tgcctggccg ttattggttt gcgctccgct atcggcagcc agtgcggcac cgctggcaag
    13321 gcttagagtg gcagtcagaa atagtgtggc cagttttgtc attttcatag gatgctcctg
    13381 ttatggtcgt tatgtcggat aacctcttcc aacagtgcat ttgcaggtga atataaggca
    13441 ttggtttaag atttcagcca ggcaaagaaa tccagcagag aatcttgaaa taattaacaa
    13501 acaaaggagt tacagttaga aattgtagga gagatctcgt ttttcgcgac aatctggcgt
    13561 ttttcttgct aattccagga ttaatccgtt catagtgtaa aaccccgttt acacattctg
    13621 acggaagata tagattggaa gtattgcatt cactaagata agtatggcaa cactggaaca
    13681 gacatgaatt atcagaacga cgatttacgc atcaaagaaa tcaaagagtt acttcctcct
    13741 gtcgcattgc tggaaaaatt ccccgctact gaaaatgccg cgaatacggt tgcccatgcc
    13801 cgaaaagcga tccataagat cctgaaaggt aatgatgatc gcctgttggt ggtgattggc
    13861 ccgtgctcaa ttcatgatcc tgtcgcggct aaagagtatg ccactcgctt gctggcgctg
    13921 cgtgaagagc tgaaagatga gctggaaatc gtaatgcgcg tctattttga aaagccgcgt
    13981 actacggtgg gctggaaagg gctgattaac gatccgcata tggataacag cttccagatc
    14041 aacgacggtc tgcgtatagc ccgtaaattg ctgcttgata ttaacgacag cggtctgcca
    14101 gcggcgggtg aattcctcga tatgatcact cctcagtatc tcgctgacct gatgagctgg
    14161 ggcgcaattg gtgcacgtac cacggaatcg caggtgcacc gcgaactggc gtctggtctt
    14221 tcttgtccgg taggttttaa aaatggcaca gacggtacga ttaaagtggc tatcgatgcc
    14281 attaatgccg ccggtgcgcc gcactgcttc ctgtccgtaa ctaaatgggg gcattcggcg
    14341 attgtgaata ccagcggtaa cggcgattgc catatcattc tgcgcggcgg taaagagcct
    14401 aactacagcg cgaagcacgt tgctgaagtg aaagaagggc tgaacaaagc aggtctgcca
    14461 gctcaggtga tgatcgattt cagccatgct aattcgtcca aacaattcaa aaagcagatg
    14521 gatgtttgtg ctgacgtttg ccagcagatt gccggtggcg aaaaggccat tatcggtgtg
    14581 atggtggaaa gtcatctggt ggaaggcaat cagagcctgg agagcgggga gccgctggcc
    14641 tatggtaaga gcatcaccga tgcctgcatt ggctgggaag ataccgatgc tctgttacgt
    14701 caactggcga acgcagtgaa agcgcgtcgc gggtaaagtt taattgtcgg atgcgccgtc
    14761 agagtggcgt atccgatgaa tcaccgtagc cctgacaaga cgcgcagcgt cgcatcaggc
    14821 aatgtgctcc attgttagcg acgacaaaca aaaaagccga ctcacttgca gtcggctttc
    14881 tcatttttaa acgaatgacg tttacttcgc tttaccctgg ttcgcaacgg ctgctgcttt
    14941 cgccgcgatc tcgtcagcat tacccagata atagcgtttc agcggtttga agttctcgtc
    15001 aaactcataa accagcggca cgccggtcgg gatattcagc tcaagaatct cttcttcgct
    15061 catgttatcg agatatttca ccagcgcacg taacgagtta ccgtgtgcag cgatgattac
    15121 gcgctcaccg ctcttcatac gcggcagaat ggtttcattc cagtaaggga tcacgcggtc
    15181 aatggtcagc gccaggcttt ccgtcagcgg cagttctttc tcgctcagtt tcgcgtaacg
    15241 cggatcgtga cccggataac gctcatcatc tttagtcagt tccggcggag tcactgcaaa
    15301 accacgacgc cactgtttca cctgctcgtc gccatatttt tcagcagttt ccgctttgtt
    15361 cagaccctgc aacgcaccgt agtgacgttc gttcagtttc caggatttct caacgggcag
    15421 ccatgcctga tccagttcgt ccagcacatt ccacagggta tggatagcgc gtttcagcac
    15481 agaggtgtaa gcaaagtcaa agctgtaacc ttcctctttc agcagcttac ctgctgcttt
    15541 tgcttcgctt acgcctttct cagacagatc cacgtcgtac caaccggtga aacggttttc
    15601 tttgttccac tgactttcgc catgacgaac cagaaccagc ttagttacag ccatatactt
    15661 actcctcaaa tcatctttta atgataataa ttctcattat attgccgtga cgaagcaaca
    15721 gcaatgctta cgcataacca tagcgaaaat agtggcgcag tgtaaggttg ttgtgaatat
    15781 tgagttgcaa atatgtcggt gtttgctggt gatttgaaca atatgagata aagccctcat
    15841 gacgagggct taacattact cagcaataaa ctgatattcc gtcaggctgg aatactcttc
    15901 accaggatgc aggaaacagt ccggttgcgg ccattcaggg tggttcgggc tgtccggcag
    15961 aaactcgctt tccaacgcca gcccttgcca gtcggcgtaa ggttcggttc cccgcgacgg
    16021 tgtgccgccg aggtagttgc cggagtagaa ttgcagagcc ggagcggtgg tgtagacctt
    16081 cagctgcaat ttttcatctg ctgaccagac atgcgccgcc actttcttgc catcgccttt
    16141 ggcctgtaac aagaatgcgt gatcgtaacc tttcactttg cgctgatcgt cgtcggcaag
    16201 aaactcactg gcgatgattt tggcgctgcg gaaatcaaaa gacgttccgg cgacagattt
    16261 caggccgtcg tgcggaatac cgccttcatc aaccggcaga tattcgtccg ccagaatctg
    16321 caacttgtga ttgcgcacgt cagactgctt gccgtcaaga ttgaaataga cgtgattagt
    16381 catattcacc gggcaaggtt tatcaactgt ggcgcgataa gtaatggaga tacggttatc
    16441 gtcggtcaga cgatattgca ccgtcgcgcc gagattaccc gggaagccct gatcgccatc
    16501 atctgaactc agggcaaaca gcacctgacg atcgttctgg ttcacaatct gccagcgacg
    16561 tttgtcgaac ccttccgggc cgccgtgcag ctggttaacg ccctgacttg gcgaaagcgt
    16621 cacggtttca ccgtcaaagg tataacgact attggcgatg cggttggcat aacgaccaat
    16681 agaggcaccc agaaacgcgg cctgatcctg atagcattcc gggctggcac agccgagcag
    16741 cgcctcgcgg acgctgccat cggaaagcgg aatacgggct gaaagtaaag tcgcacccca
    16801 gtccatcagc gtgactacca tccctgcgtt gttacgcaaa gttaacagtc ggtacggctg
    16861 accatcgggt gccagtgcgg gagtttcgtt cagcactgtc ctgctccttg tgatggttta
    16921 caaacgtaaa aagtctcttt aatacctgtt tttgcttcat attgttctgc gacagcttgc
    16981 tgtacggcag gcaccagctc ttccgggatc agcgcgacga tacagccgcc aaatccgccg
    17041 ccggtcatgc gtacgccacc tttgtcgcca atcacagctt tgacgatttc taccagagtg
    17101 tcaatttgcg gcacggtgat ttcgaaatca tcgcgcatag aggcatgaga ctccgccatc
    17161 aactcgccca tacgtttcag gtcgccttgc tccagcgcgc tggcagcttc aacggtgcgg
    17221 gcgttttcag tcaggatatg acgcacgcgt ttcgccacga ttgggtccag ctcatgtgca
    17281 acagcgttga actcttcaat ggtgacatcg cgcagagctg gctgctggaa gaaacgcgca
    17341 ccggtttcgc actgttcacg acgggtgttg tattcgctgc caaccagggt acgtttgaag
    17401 ttactgttga tgatgacgac agccacacct ttcggcatgg aaactgcttt ggtccccagt
    17461 gagcgacagt caatcagcaa ggcatgatct ttcttgccga gtgcggaaat tagctgatcc
    17521 atgatcccgc agttacagcc aacaaactgg ttttctgctt cctgaccgtt aagcgcgatt
    17581 tgtgcgccgt ccagcggcag atgataaagc tgctgcaata cggttccgac cgcgacttcc
    17641 agtgaagcgg aagaacttaa cccggcaccc tgcggcacat tgccgctgat caccatgtcc
    17701 acaccgccga agctgttgtt acgcagttgc agatgtttca ccacgccacg aacgtagttc
    17761 gcccattgat agttttcatg cgcgacaatg ggcgcatcaa gggaaaactc atcaagctga
    17821 ttttcataat cggctgccat tacgcgaact ttacggtcat cgcgtggcgc acagctgatc
    17881 acggtttgat aatcaatcgc gcagggcaga acgaaaccgt cgttgtagtc ggtgtgttca
    17941 ccaatcaaat tcacgcggcc aggcgcctga atggtatgag tggcagggta gccaaatgcg
    18001 ttggcaaaca gagattgtgt tttttctttc agactcattt cttacactcc ggattcgcga
    18061 aaatggatat cgctgactgc gcgcaaacgc tctgctgcct gttctgcggt caggtctcgc
    18121 tgggtttctg ccagcatttc ataaccaacc ataaatttac gtacggtggc ggagcgcaac
    18181 agaggcggat aaaagtgcgc gtgcagctgc cagtgttgat tctcttcgcc attaaacggt
    18241 gcgccgtgcc agcccataga gtaggggaag gagcactgga agaggttgtc ataacgactg
    18301 gtcagctttt tcaacgccag tgccaaatcg ctgcgctggg cgtcggtcaa atcggtgatc
    18361 cgcaaaacgt gggctttggg cagaagtagc gtttcgaacg gccaggcagc ccagtaaggc
    18421 acaactgcta accagtgttc ggtttcgaca acggtacggc taccgtctgc cagctcgcgc
    18481 tgaacataat ccaccagcat tggcgatttc tgctcggcaa aatattcttt ttgcaggcgg
    18541 tcttcgcgct cagcttcgtt aggcaggaag ctatttgccc aaatctgtcc gtgcggatgc
    18601 gggttagagc agcccatcgc cgcacctttg ttttcaaaga cctgcaccca cgggtatgtt
    18661 ttccccagtt ctgcggtttg ctcctgccag gttttgacga tttccgtcaa tgccgcaacg
    18721 ctcagttctg gcagcgtttt actgtgatcc ggtgaaaagc agatcacccg gctggtaccg
    18781 cgcgcgctct ggcaacgcat caaaggatcg ttgctttctg gcgcatctgg cgtgtcagac
    18841 atcaaggccg caaagtcatt agtgaaaacg taagtcccgg tgtaatcggg gtttttatcg
    18901 cctgtcaccc gcacattacc tgcgcagagg aagcaatctg gatcgtgcgc aggtaacacc
    18961 tgtttggctg gcgtttcctg cgccccctgc caggggcgct tagcgcggtg cggtgaaacc
    19021 agaatccatt gcccggtgag ctggttgtag cggcgatgtg gatgatcaac gggattaaat
    19081 tgcgtcatgg tcgttcctta atcgggatat ccctgtggat ggcgtgactg ccagtgccag
    19141 gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcgcc agttcagttc acggtcggct
    19201 ttgctggcgt ccgcccagta ggccggaagg tcgccctcgc gacgcggtgc aaaatgatag
    19261 ttaaccggtt tgccgcaggc tttgctgaag gcattaacca cgtccagcac gctgctgcct
    19321 acgccagcac cgaggttgta gatgtgtacg cctggcttgt tcgccagttt ttccatcgcc
    19381 acgacgtgac cgtccgccag atccattacg tggatgtaat cgcgtacgcc agtaccgtct
    19441 tcggtcggat aatcgttacc aaaaatcgcc agcgagtcgc gacggcctac agcaacctgg
    19501 gcgatgtatg gcataaggtt attcggaatg ccttgcggat cttcgcccat atcgcccgac
    19561 ggatgtgcgc caaccgggtt gaagtagcgc agcagggcaa tgctccagtc cggctgggct
    19621 ttttgcagat cggtgaggat ctgttccacc atcagcttgc ttttgccgta agggctttgc
    19681 ggtgtgccgg tcgggaagct ttcaacgtat ggaattttgg gctgatcgcc ataaacggtg
    19741 gcggaggagc taaaaataaa gtttttgacg ttagcggcgc gcatggcgct aatcaggcgc
    19801 agagtaccgt tgacattgtt gtcgtaatat tccagcggtt tttgtaccga ttcgccaacg
    19861 gctttcagcc cggcgaagtg gatcacggtg tcgatagcgt gatcgtgcag gatctcggtc
    19921 atcaacgctt cgttacggat atcgccttca acaaacgtcg gatgtttgcc gcctaaacgc
    19981 tcgataacag gcagtacgct gcgcttactg ttacagaggt tatcaagaat gatgacatca
    20041 tgaccgtttt gcagtaattg cacacaggta tgacttccaa tgtaaccgct accaccagtg
    20101 accagaactc tcataattcg ctccattagg cttatggtat gaaataacca tagcataaca
    20161 aagatgcgaa aagtgtgaca tggaataaat tagtggaatc gtttacacaa gaatttagcc
    20221 gttttttatg cgcgattaag tgattataaa acagagggat tgtgaatgat tgcgcttttt
    20281 atctgaaaaa aagacgcagt ttcatgcctg gatgcgtctg accgtgtgcc ggataaagcg
    20341 tttacgccgc atccggcaca gtcagcatta ccgacttagt ttatttttgt cagcgcataa
    20401 tgatagaggc caccgtccgg cacgaactcc agacgatggg taatacaagc cggcgcatct
    20461 tcagcatggt gcgaaacaaa caacaattgc gtttcacctt cgctaatcag cacatcaaca
    20521 aaacggcgaa taagctggcg attgagcgga tcaagcccct gcaatggttc gtcgagaata
    20581 agcaacgtcg gatgtttcac cagtgcgcgg acgatcagcg ccagacgctg ctgtccccag
    20641 gaaagactat ggaacggagc gtcagccgtg cgtttatcaa tgccgagaat atccagccac
    20701 tgctgcacca gtttttgctg gcgatccgaa acggcctgat aaatgccaat cgaatcaaaa
    20761 tagccagaaa gaatcacatt acgcacagta gtactgaccc ggtaatccag atgcaaacta
    20821 ctgctgacgt aaccgatatg ctttttgata tcccagatgg tttcgccgct gccacgacgt
    20881 cgtccgaaaa gcgtcaaatc gttgctgtaa ccttgcggat gatcgccagt aatcaggctt
    20941 aataacgtcg attttcccgc gccatttggc ccgacaattt gccagtgttc acctggattc
    21001 acctgccagc taaggttatt aagaatgggg cgatcgttat aagaaaccac gccattattc
    21061 agcacaatgc gcggttcgtt ggcgggtaag gcgtgacgtg ctgaaggttc atccggctcc
    21121 ggcagttgca caccttcaag ctgttcacta tgcgccagtt gcgcgacgag tgcttgttgg
    21181 agcagttcct ctttagcgcc agtttccgct aacgtgcaat ccgccagcac gccagcaaac
    21241 tggacaaact ccgggatctc atcgaagcga ttgagcacca gtaccagagt aataccggac
    21301 tgatgtaacg aggcgagtcg ctcagccagc tgctgacgtg aggcaacatc caggccatcg
    21361 aacggctcat caagaatcaa caagtcaggc tccgacatca gcgcctgaca cagcagggtt
    21421 tttcgcgtct cgccagtgga aaggtattta aagcgtcggt cgaggagggg ggtaataccg
    21481 aactgctgtg ccagttgcat gcagcgcggt gcatccttta cttcatcctg aataatctca
    21541 gccgtagtgc gtccggtgtc atcttcgcca gggccgagca tatcggtgtt attccgctgc
    21601 cattcgtcgc tgacgagctt ttgcaattgc tcgaaggaga gacgagtgat gtgggaaaac
    21661 tggctttgcc gttcaccttt caaaagcgga agttcccccg ccagcgcgcg ggccagggcc
    21721 gatttcccgc ttccattcga accgacaaac gcccaactat cacccgcgtt taacgttagc
    21781 tgaggcaatt gcagcgtttt tgtgtcgcta agacgaaacg tgccttgcaa aatttgcaac
    21841 gatgacattt tatatcccat tttgtgcagc gattactgac agggatacgt gtttcataac
    21901 aaattgtcaa cacgcttagc acagtgtggc gataatcacg ctgtcggcat taaagtaggc
    21961 cgtgacattc tgtccttgct gaagagaagt cgcttcattt accggcactg tggcgcacag
    22021 tgtttgcccg tccggtagcg ccattaatac ttcgcactgc tctgcgccgc gctcaatatg
    22081 actaataata cccggtaatt ggttgtcagc gttttgcgcg accgcttcgt cctgagtaat
    22141 gcctacccac ggcgctttta gcaatatcaa cacttctttg ccttcatcca gccccagacg
    22201 cgcgccgctt tgtgcggtaa ttgcgacttt caggcgtgtt tttccatcag ccagtaagac
    22261 atcaacatgc tgttgaacgt catcatgatc gcgggcggtg atggtaccga accactggtt
    22321 acgggcgctg gtttggagtg aaaaacgtga gatcgcggcc agcaggctgt tcagcggcag
    22381 ggcgtcatcg tcacttaaca catcaaaggc tttttgctgg atttgcgcca gtaagtcata
    22441 gagctgaatc agtcgctgac catagcgggt cagtactgcg ccgccgccac ctttaccgcc
    22501 tgttgcgcgc tcgaccagaa tatgctcact taactgattc atctcgttaa tggcatccca
    22561 ggcgctttta tagctgatac cggcatcttt cgctccctgg ctaatggaac cggaaagcgc
    22621 aatgtgtttt agtagcgaaa tgcggcgcgg gtcggcgaat aatttttgtt ggagcttaag
    22681 ggtgagaagg atttcggcct gcataacaat gtcctggcaa aagtcttatt gtgacggaaa
    22741 acgaacgcca cgcaaagctg accgcacaaa aggggagtgc ttttctgtgc ttagcggtta
    22801 gaatagtctc atgactatat ctggagttga ccatgttaga gttattaaaa agtctggtat
    22861 tcgccgtaat catggtacct gtcgtgatgg ccatcatcct gggtctgatt tacggtcttg
    22921 gtgaagtatt caacatcttt tctggtgttg gtaaaaaaga ccagcccgga caaaatcatt
    22981 gattccctga atgcccgctt agtcgggcat tttctttttc tcaacttcct gcttttcctg
    23041 ccgatatttt ttcttatcta cctcacaaag gttagcaata actgctggga aaattccgag
    23101 ttagtcgtta tattgtcgcc tacataacgt tacattaagg ggttaccaat ggctcgtaaa
    23161 tggttgaact tgtttgctgg ggcggcactc tctttcgctg ttgctggcaa tgcactggca
    23221 gatgaaggga aaatcacggt gttcgccgcc gcatcactga ctaacgcaat gcaggacatt
    23281 gctacgcagt ataaaaaaga gaaaggcgtg gatgtggttt cttctttcgc ttcgtcatct
    23341 actctcgccc gtcagattga agcgggtgcc cctgcggatc tgtttatttc tgccgatcag
    23401 aaatggatgg attatgcggt tgataaaaaa gcgatcgata cagctacgcg tcagacactg
    23461 ctcggcaata gcctggtcgt tgtagcaccg aaagccagcg agcagaaaga ttttaccatc
    23521 gacagcaaaa ccaactggac ttcactgctg aatggcggtc gcctggcggt tggcgatccg
    23581 gaacatgttc ccgctggcat ttatgcaaaa gaagcactgc aaaaactggg cgcatgggat
    23641 acgctctccc cgaaactggc tccggcggaa gatgttcgtg gcgcgctggc gttggtcgaa
    23701 cgcaatgaag cgccgttggg cattgtttac ggttctgacg cagttgctag caaaggggta
    23761 aaagtggttg ccaccttccc ggaagattca cataaaaaag tggaatatcc ggttgctgtt
    23821 gtggaagggc ataacaatgc gacagtgaaa gcattttatg attatctgaa gggaccgcag
    23881 gcagcggaaa tctttaaacg ttacggattt acaaccaagt aatgatactg accgatccag
    23941 aatggcaggc agttttatta agcctgaaag tttcttccct ggctgtgctg tttagcctgc
    24001 cgtttgggat cttttttgcc tggttactgg tgcgttgcac gtttccgggc aaagctctgc
    24061 tcgacagcgt actgcatcta ccgctggtgt taccgcccgt ggtcgtcggt tacttattat
    24121 tagtttcgat ggggcggcgc ggatttatcg gtgaacgtct gtatgactgg tttggtatta
    24181 ccttcgcctt tagctggcgc ggcgcggttc tcgctgccgc cgtcatgtcg tttccgctga
    24241 tggtgcgggc aattcgtctg gcgctggaag gggttgatgt caaactggaa caggccgcaa
    24301 gaacactggg ggccgggcgc tggcgcgttt tctttactat cacgttaccg ctgaccttac
    24361 cgggaattat tgttggtacg gtactggctt ttgctcgttc tctcggtgaa tttggcgcaa
    24421 ccatcacctt tgtgtcgaac attcctggtg aaacgcgaac cattccttct gccatgtata
    24481 ccctgattca gacccccggc ggggaaagtg gcgcagcgag actgtgcatt atttctattg
    24541 cgctggcgat gatctccctg ttgatttcag aatggctggc cagaatcagc cgtgaacggg
    24601 cggggcgcta atcatgctgg aactgaattt ttcccagacg ttgggcaacc attgcctgac
    24661 tattaatgaa acgctgcccg ccaatggcat cactgctatc tttggcgtct ccggtgccgg
    24721 aaaaacttcg ctgattaacg ccatcagtgg actgacgcgc ccgcaaaaag ggaggattgt
    24781 cctcaatggt cgggtactaa atgatgccga aaaaggtatc tgcctgacgc cggaaaagcg
    24841 tcgcgttggc tatgtttttc aggatgcgcg gctgttcccg cattacaaag tgcgtggcaa
    24901 tctgcgctac ggcatggcga aaagcatggt cgatcagttc gataagctgg tggcgctttt
    24961 aggcattgaa ccgttgcttg accgtttacc aggcagcctg tccggaggcg aaaaacagcg
    25021 cgtagcgatt gggcgggctt tgctgacggc accagaattg ctgttgctgg atgaaccgct
    25081 ggcgtcactg gatattccgc gtaaacgcga actgttgcct tatctgcaac gtctgacgcg
    25141 ggaaatcaac atcccgatgt tgtatgtcag ccattcgctg gatgagatcc tccatctggc
    25201 agacagagtg atggtactgg aaaacggtca ggtgaaagcc tttggcgcgc tggaagaagt
    25261 ctggggtagt agcgtgatga atccgtggct gccgaaagag caacaaagta gcattctgaa
    25321 agtgacggta cttgagcatc atccgcatta cgcgatgacc gcgctggcgc tgggcgatca
    25381 gcatttgtgg gtcaataagc tggacgaacc gctgcaagct gcgctacgta tccgcattca
    25441 ggcttccgat gtttctctgg ttttacaacc gccgcagcaa accagcattc gtaacgtatt
    25501 gcgggcaaaa gttgttaata gttatgacga caacggccag gtggaagtgg aactggaagt
    25561 cggcggtaaa acgctgtggg cgcgtatcag cccgtgggcc agggatgaac tggcgatcaa
    25621 acctggcctg tggctgtacg cgcaaattaa aagtgtgtcg ataaccgcct gattaaatca
    25681 ggtggctata aatgaactgg gcaatgctgt cggtggtgtt atcaccaatc acaatgttgg
    25741 cgcgcgcttt taccgcgtca tcggcgttgc ccatcgccac gcctgtacca gcggcctcca
    25801 gcatactgat atcattaaag ttatcgccga acgccacgac gttttccatc gaccagcctt
    25861 gcgcctcaac ccatttcgtc aaacgtttac ctttgctgtt gccgccgcgt gcaatatcaa
    25921 cctgatcgtg ccaggaccat tcacactcca gtcccagttc atgttcgaca tgcttaccaa
    25981 aatgctgcaa ttgcggcagg tcatcgtgcg tgagggcgaa cttccatacg gcgttaactt
    26041 gttgcgccgt ttcagccaga gaagcgactt gtgtgaaagt cggacgctgt tccggcggca
    26101 gggtttgcgc ccagttagac gtgcgaatga catgcccggt cgggtgctca taaaccatct
    26161 cgtcatcgac atacatcaga ccgtgaatgt ggtgttcatt cagcatctca atgagttgta
    26221 gggctttatt aacgggcatt gggtccgctt ccagcacggt ttttgcatga taatcataca
    26281 aataggtgcc attacagcaa atagcaggtg tatccagcgc cagcgcctga taaaaaggat
    26341 gaatagcgac gtgatggcga cctgtgacga tgattaattg ataacctgct tcgcgagcgc
    26401 gggccagggc ttctatcgat gaaggtagca gggtcttttt cggggtcaat aaggtgccgt
    26461 ctaagtcgag agcaatcacg cgtgtggtca tggcgtattc cagattaagg ttaagaattt
    26521 tcttctgcgc gaatggtaca ccgataccac tatcggacaa aattctgcgt tttaattcag
    26581 cattcaccgc caaaagcgac taattttagc tgttacagtc agttgctaaa tgcaaaggag
    26641 cattcatgaa gcaaacagtt tatatcgcca gccctgagag ccagcaaatt cacgtctgga
    26701 acctgaatca tgaaggcgca ctgacgctga cacaggttgt cgatgtgccg gggcaggtgc
    26761 agccgatggt ggtcagcccg gacaaacgtt atctctatgt tggtgttcgc cctgagtttc
    26821 gcgtcctggc gtatcgtatc gccccggacg atggcgcact gacctttgcc gcagagtctg
    26881 cgctgccggg tagtccgacg catatttcca ccgatcacca ggggcagttt gtctttgtag
    26941 gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga agatggcctg ccagttggcg
    27001 tcgtcgatgt ggtcgagggg ctggacggtt gccattccgc caatatctca ccggacaacc
    27061 gtacgctgtg ggttccggca ttaaagcagg atcgcatttg cctgtttacg gtcagcgatg
    27121 atggtcatct ggtggcgcag gaccctgcgg aagtgactac cgttgaaggg gccggcccgc
    27181 gtcatatggt attccatcca aacgaacaat atgcatattg cgtcaatgag ttaaacagct
    27241 cagtggatgt ctgggaactg aaagatccgc acggcaatat cgaatgtgtc cagacgctgg
    27301 atatgatgcc ggaaaacttc tccgacaccc gttgggcggc tgatattcat attaccccgg
    27361 atggtcgtca cttatatgcc tgtgaccgta ccgccagcct gattactgtc ttcagcgttt
    27421 cggaagatgg cagcgtgttg agtaaagagg gcttccagcc aactgaaacc cagccgcgcg
    27481 gctttaatat cgatcaccgt ggtaagtatc tgattgccgc cgggcaaaaa tcgcaccaca
    27541 tctcggtata cgaaattgtt ggtgagcagg ggctactgca tgaaaaaggc cgctacgcgg
    27601 tcgggcaggg accaatgtgg gtggtggtta acgcacacta accgctgatt tacccggcgc
    27661 agtctctcct gcgccggtgt attaacctat ctcctgtaac gcatgtctct ggcgttcgac
    27721 gatattggtc cacaaattgt ctttatcgtc agtccacaaa ttaatcagca aggcaaaaaa
    27781 gcgttctgct gccggagaaa gcacggcatc tttgcggcga ataatcccca atgtccgacg
    27841 tataacgggt tccaccagtg ggataccaat aatagaagaa tagggcgcat ggggcattgc
    27901 cagaccagga agcgccgaaa ttcccagtcc cgcctctacc agtcctaatg acgtcgaaag
    27961 atgacgcact tcgtaaaacc agtccagctt ccagggcttg tcggctagct gttgttctat
    28021 caacaatcgg ttgccactgg aggaacgtac gccaatcatt ttgtaaccca ctaactcctg
    28081 ccattctaca agctgctttt tagccaatgg atggtcacgc cgacaggcca gcacgaacgg
    28141 ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc
    28201 aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat
    28261 ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata
    28321 aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc
    28381 acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct ctgggagcaa
    28441 cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg
    28501 taaatcttcc tccatctttt ttattcttcg tgttaatgca ggttgcgtaa tattgagtaa
    28561 tttagcggca ttgttaaatg aactggactc tgccagtatg acaaatgcct tcatacttga
    28621 taattcatgc ttcattatta ctccgggaaa tggaagcgac gattttgggt ggctggccgt
    28681 taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga
    28741 gctaaagtca ttagagatgc tttgccctta atgtaaccat atcgcaataa gttatgtttt
    28801 taaattgagg gcattattat gaaaaaaata ccctgcgtga tgatgcgagg tggaacctcg
    28861 aggggcgcgt tcctgttagc ggaacattta cccgaagatc aaacgcagcg cgataaaata
    28921 ttgatggcaa ttatgggttc cggtaacgat ctggaaattg acggtattgg cggcggtaat
    28981 ccactgacca gtaaagtcgc cattattagc cgttccagcg atccgcgtgc tgatgttgat
    29041 tatctgtttg ctcaagttat cgtccatgag aaacgtgtcg ataccacgcc taactgcggc
    29101 aatatgctgt ctggcgttgg ggcattcgcc attgaaaatg gtttgattgc agcgacttcg
    29161 ccagttaccc gcgtacgtat ccgcaacgtc aatacgggta cgttcatcga agctgatgtg
    29221 caaacgccaa atggtgttgt cgagtacgag ggtagcgcca gaattgacgg cgtaccgggt
    29281 actgccgcac cggttgcgct cactttcctg aatgccgctg gaacgaaaac cggaaaagtt
    29341 ttcccgactg ataatcagat tgattatttt gacgatgtcc cggtgacctg tatcgatatg
    29401 gcgatgccag tcgtcattat tccggctgaa tatctgggaa aaacaggcta tgaattaccg
    29461 gcggaactgg atgccgacaa agcattatta gcccgcattg aatctatccg tctacaagcg
    29521 ggtaaagcaa tgggcttagg cgatgtcagt aatatggtta tccctaaacc tgtgcttatt
    29581 tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt ttatgccgca ttcttgccat
    29641 cgcgcgctgg cgataaccgg tgctattgct atttccagta gttgtgcatt ggaaggtacc
    29701 gtcacccgac aaatcgtccc ttctgtagga tacggcaata tcaatattga acaccccagt
    29761 ggtgggctcg acgttcattt aagtaatgaa ggtcaggatg ccacgacgtt acgcgcatct
    29821 gttattcgga caaccagaaa aatattttcc ggtgaagttt atcttccctg aaaagattcg
    29881 ttgtcaggat aaggacaatc aataaaggac ttctgtatga gtcatacaga aagaacagga
    29941 ttttaaatga ataagaaatc gttatggaag ctaattctga tattagcgat cccatgtatt
    30001 attggcttta tgccagctcc ggcaggatta agcgaactgg cgtgggtgct ttttggtatt
    30061 tacctggcgg ccattgtggg gctggttatc aagcctttcc cggaacctgt cgtactgtta
    30121 attgccgttg ctgcctccat ggtggtggtc ggtaacttat ccgacggtgc gtttaagacc
    30181 accgccgtat taagcggtta ctcttcaggt actacctggc tggtgttctc ggcgtttacc
    30241 ttaagcgccg catttgtgac caccggttta ggtaaacgta ttgcctatct gctgattggt
    30301 aaaatcggta acaccacgct gggtctgggt tacgttacgg tattcctcga tctggtattg
    30361 gctccggcaa caccgtctaa caccgcgcgt gcgggcggca ttgtgttacc gatcatcaac
    30421 agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc cgcgtcgtgt cggacattac
    30481 ctgatgatgt ccatttacat ggtcaccaaa accaccagct atatgttctt taccgcaatg
    30541 gcggggaaca ttctggcgct gaaaatgatc aacgacattc tgcacctgca aattagctgg
    30601 ggtggatggg cgctggccgc cggattgccg ggcatcatta tgctgctggt caccccgctg
    30661 gtgatttaca ccatgtatcc accagaaatt aagaaggtgg ataacaaaac catcgctaaa
    30721 gcgggccttg ccgaactggg accgatgaaa atccgcgaaa aaatgctgct cggtgtcttt
    30781 gtgctggcgc tgctgggctg gattttcagt aagtctctgg gggttgatga atcttcagtg
    30841 gctattgttg ttatggcgac catgctgctg ctgggtatcg ttacctggga agacgtggtt
    30901 aaaaataaag gcggctggaa taccttaatc tggtacggcg gtattatcgg cttaagctcc
    30961 ttattatcga aagttaaatt tttcgaatgg ttagctgaag tctttaaaaa taacctggca
    31021 tttgatggtc acggtaacgt tgctttcttc gttattattt tcctcagcat catcgtgcgt
    31081 tatttcttcg cttccggtag tgcctatatc gttgctatgt taccggtatt tgccatgctg
    31141 gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg cactgttgtt ctccaactcc
    31201 tatggcggca tggttactca ctatggcggc gcggcaggtc cggtcatctt tggcgtgggt
    31261 tataacgata ttaaatcctg gtggttggtc ggtgcggtac tgacgatatt aaccttcctg
    31321 gtgcatatca ccctcggcgt gtggtggtgg aatatgctga tcggctggaa catgctgtaa
    31381 atatactcgt catacttcaa gttgcatgtg ctgcgtctgc gttcgctcac cccagtcact
    31441 tacttatgta agctcctggg gattcactcg cttgtcgcct tcctgcaact cgaattattt
    31501 agagtatatc catttatttt cttgctgcgc acttcacggt gcgcagatat ctggagcatt
    31561 tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag taataacgaa ataattgccg
    31621 aagaacattt caccggcgaa attaaaaaag aagaagcaaa aaaaggcact attgcctggt
    31681 ctattctctc ttctcataat acgtccggaa atatggataa acttaaaatt aagtttgatt
    31741 cattagcctc tcacgatatt acctttgttg gtattgtaca gaccgctaaa gcgtccggta
    31801 tggaacgttt cccgctgccg tatgtgctga ccaactgcca taactcactc tgcgccgtcg
    31861 gcggcaccat taacggtgat gaccatgttt ttggtttatc ggcagctcag cgttatggcg
    31921 gtatttttgt gcctccgcat attgcggtca tccatcaata tatgcgtgag atgatggcag
    31981 gtggcggcaa aatgatcctc gggtcagaca gtcacacccg ttacggtgca ttagggacaa
    32041 tggcagtcgg tgagggcggc ggcgagttgg taaaacaatt gcttaatgac acctgggata
    32101 tcgactatcc gggagttgtt gcggtgcatc tgaccggaaa accagcgccg tatgtggggc
    32161 cgcaggatgt ggcgttggct atcatcggtg ccgtgttcaa aaacggctac gtcaaaaaca
    32221 aagtgatgga attcgtaggt cccggtgttg ctgcgctctc taccgatttc cgtaacagcg
    32281 ttgacgttat gaccactgaa acgacctgtt taagttctgt ctggcaaacc gatgaagaag
    32341 tccataactg gctggcgctg cacggtcgcg gccaggatta ctgccagctt aaccctcaac
    32401 cgatggcgta ctacgatggc tgcatcagcg ttgatttaag cgccatcaaa ccaatgattg
    32461 cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac actgaaccag aacttgaccg
    32521 acattctgcg tgagattgaa attgagtccg aacgcgtggc gcacggtaaa gccaaactct
    32581 cgctgctgga caaagtggaa aatggtcgcc tgaaagtgca gcaggggatt atcgcgggct
    32641 gttctggcgg taactacgaa aacgtcatcg cggcggcgaa tgcactgcgc ggtcaatcct
    32701 gtggcaatga caccttctcg ctggcggttt acccgtcatc acagccggtg tttatggatc
    32761 tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg cgcaatcatc agaaccgcgt
    32821 tctgcggccc atgctttggc gcgggcgata cgccaatcaa caacggtttg agtattcgcc
    32881 acaccacgcg taacttcccg aaccgcgaag gctctaagcc agctaatggg cagatgtcag
    32941 cggtggcgtt gatggacgct cgttctatcg ctgcgactgc ggcaaacggt ggctatttaa
    33001 cctctgccag cgaactcgat tgctgggaca acgtgccgga gtacgccttc gatgtaacgc
    33061 cgtataaaaa tcgcgtctat caaggctttg tgaaaggggc ggcccagcaa ccgctgattt
    33121 acggaccgaa cattaaagac tggccggaat tgggtgcgct gactgacaat atcgtcctca
    33181 aagtgtgctc gaagatcctc gacgaagtga ccaccaccga cgaattgatt ccttccggtg
    33241 aaacctcttc ttatcgttca aatccgattg gtctggcgga gtttaccctg tcacgccgcg
    33301 atccaggtta tgttggcagc agtaaagcga ctgctgagct ggaaaatcag cgtctggcgg
    33361 ggaatgtcag cgagctgaca gaggtgtttg cgcgcattaa gcagattgct ggtcaggagc
    33421 atattgatcc gctgcaaact gaaattggca gcatggtcta tgcggtgaaa ccaggcgatg
    33481 gttctgcgcg tgaacaggcg gcgagttgtc agcgtgtgat tggcggtctg gcgaatattg
    33541 ccgaggagta cgcgactaaa cgctatcgtt ctaacgtcat caactggggg atgttaccgc
    33601 tgcagatggt ggaagcgcca acctttgaag tgggggatta catttacatc cctggcatta
    33661 aagcggcgct ggataatccg ggtacgacgt ttaaaggtta tgtgatccat gaagatgcgc
    33721 cggtaacgga aattacgctc tatatggaaa gtctgactgc tgaagagcgc gagattatca
    33781 aggcgggtag tttgattaac ttcaataaaa accgtcagat gtaaaaagcg ccatgtgaat
    33841 gtaggtcgca ttcggcactt attgtcggat gcgatgctgg cgcatcttat ccgacctaca
    33901 aatcgcatcg aaccgtaggc cggataaggc gtttacgccg catccggcaa atagttaata
    33961 gctcttactt cttcgcctct gcaaccactt tgctacccac gccgcggtta ttgtattccc
    34021 acatgcggtt gtagttagtg tcattcagat tgcgctgtat ttcgtcgcta tcgtcaacgc
    34081 tgccggtgtt acccgcaaac ggacgattag agatcaccgc atcgccccac ggtttagccg
    34141 tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg gtattggcat
    34201 caacatccag cgagcggccc agttgtgcca cgccatcacc ggaagcattg aaacggctgt
    34261 ttacggcgag gaaaccgtag taaatgttgg acagcgtagc cggtgcaaac acatacgctt
    34321 cttgctgagt acgtgagttc accacgcgga attcggtgtt atcgaacacc actgcgccgc
    34381 gaccagaaac gatatccaca tccccttcaa tgtagctgtt ggtcaccagc gtacgcggct
    34441 gacgattcgt ttccagacgg ttctgcacac cgctgttggt gacaaagaag gtgttctgac
    34501 gaccgagaat gttaacgttg ttaatctgta cctggtcacc gtcagtacgc agtgccaccg
    34561 ccggatggtt acccgcatct acgctatcac ccagcgtgtt ttcgatggtc aggttttgca
    34621 gttgcaggcc attgttttgt gaccagaaga ccgcagaaca cagaacaccg atactgtcgc
    34681 tgcgtttgct ctggcagcta tcgtacatat accacgctgg tttacctggc atatatttgc
    34741 cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat gcccccatca agggaaagcc
    34801 caatcttcac atcaatcggt ttttcacctg taccgtacag agtaattcca cctggagcag
    34861 cagggacata caccgttccc tgatactcac caggcatcac ggcaatatac tggcgcttgt
    34921 tggtgcgctt gataattgcc gcatctaccg ccgcctgaat cgtggtatgc gttacacctt
    34981 gagtacccgc cgggccgaca acaaagtcag gttgcgcagg cagggttatc ggggacggat
    35041 tccacgctgc agcacctggt gtcagggatg caaaatagtg ttgagcatcg aaattctgcg
    35101 cttcttttgc cgacagaatc gggcgagaag aggtaccagg cgcagtttga tcagaaggac
    35161 gttgatcggg tggtgttgag ctacaggcgg tcagcgtcac gccaaaagcc aatgccagcg
    35221 ccagacggga aactgaaaat gtgttcacag gttgctccgg gctatgaaat agaaaaatga
    35281 atccgttgaa gtctgctttt ttatactaag ttggcattat aaaaaagcat tgcttatcaa
    35341 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttga tttcaatttt
    35401 gtcccactcc ctgcctctgt catcacgata ctgtgatgcc atggtgtccg acttatgccc
    35461 gagaagatgt tgagcaaact tatcgcttat ctgcttctca tagagtcttg cagacaaact
    35521 gcgcaactcg tgaaaggtag gcggatcccc ttcgaaggaa agacctgatg cttttcgtgc
    35581 gcgcataaaa taccttgata ctgtgccgga tgaaagcggt tcacgacgag tagatgcaat
    35641 tatggtttct ccgccaagaa tctctttgca tttatcaagt gtttccttca ttgatatccc
    35701 gagagcatca acatgcaatg ttgtagggat ggcaattttt acgcctgttt tgctttgttc
    35761 gacataaaga tatccatcta cgatatcaga ccacttcatt tcgcataaat caccaactcg
    35821 ctgcccggta acaacagcca gttccattgc aagtctgagc caacatggtg atgattctgc
    35881 tgcttgataa attttcaggt attcgtcagc cgtaagtctt gatctcctta cctctgattt
    35941 tgctgcgcga gtggcagcga ccgggtttgt tgttatatgg ccttcagcta ttgcctctcg
    36001 gaatgcatcg ctcagtgttg atctgattaa cttggctgac gccgccttgc cctcgtctat
    36061 gtatccattg agcattgccg caatttcttt tgtggtgatg tcttcaagtg gagcatcagg
    36121 cagccccctc cttattgctt taattttgct catgtaattt atgagtgtct tctgcttgat
    36181 tcctctgctg gcgaggattt tttcgtagcg atcaagccat gaatgtaacg taacagaatt
    36241 atcactgttg attctcgctg tcagaggctt gtgtttgtgt cctgaaaata actcaatatt
    36301 ggcctgtatt gcttcagtga ttgctatcct cctgtctcgg cctaaaccaa actctttacc
    36361 cgtccttggg tccctgtagc agtaatattg tttcttatat aaaggttagg gggtaaatcc
    36421 cggcgctcat gacttcgcct tcttcccatt tctgatcctc ttcaaaaggc tacctgttac
    36481 tggtcgattt aagtcaacct ttaccgctga ttcgtggaac agatactctc ttccatcctt
    36541 aaccggagga gggaatatcc tgcattcgcg cacccatcga cgaactgttt caaggcttct
    36601 tgggcgtcgc tggcgtgcgt tccactcctg aagtgtcaag tacatcgcaa agtctccgca
    36661 attacacgca agaaaaagcc gccatcaggc ggcttggtgt tctttcagtt cttcaattcg
    36721 aatattggtt atgtctgcat gtgctatctg cgcccatatc atccagtggt catagcagtc
    36781 attgatgttc tctgcttcga taactctgtt gaatggttct ccattccatt cacctgtgac
    36841 tcggaagtgc atttatcatc tccataaaac aaaacccgcc gtagcgagtt cagataaaag
    36901 aaatcctcgt cagtgcgagg atgctgttca ttgctgctat acactttttt gctctcaacg
    36961 taagcggtag ctcattctgt tgggttggtg cagttgcttt taggaaatgc tatttacccc
    37021 ttaaacgtcg gctgaaagag ctaaaatcca tgcaaaaaat ttacgcaatt ttgtgtatta
    37081 ttgtgcagta agtaatgagc tattttctgc gcaaaaaatg gatggtaaat ttgtccgggg
    37141 caggaaaaat tttatgggcg ctaaacatga aaaaagattc gtatccttat ttgatttgca
    37201 tgacagtttt aggcctgatc tttattttcc ttttcttctg gtggcgggca gatatttaca
    37261 gggtcacgtt tcttaatcag agtatatccc actattacat tctgtttagc atgggaatag
    37321 cttttctgtt atctctgttt tgggttaaga aggggatagt aaaacaaagc ggctggaaga
    37381 gtctgtcagc ataccttaag gtttatgcag ggatgtgcat atttgctgga ttttttctga
    37441 ttatacccct tacaacacta acttattttt tgcctggaga gacatcgtct tatgttgcac
    37501 cgtatcggta tacttccggt agttcaaaaa gttgttctgg agctgaggtg gatgaccccg
    37561 atctacatga gaatattcgc atttgctatc cgtatggcaa ttatgagtac gataatatta
    37621 tctatgttga aaagaaaatt aatacattag gtgcggtagt aacatatgca cagaccgcgc
    37681 gtgatgatac tgaatgagat agtatatagc gggcaagttt tagttaattt atcgaggtaa
    37741 tataatttac ctcgactcgt ttattctggt attaatattt cgctttacga tcgattttta
    37801 tctgatgata tcatgcggtt ttcatatact gacttactgt cttttctccg ttagcgattt
    37861 tctcctgctc agcgatgatt ttatctttgg cttctagtta atttcgctca cttcgaacct
    37921 ctctgtttac tgataagttc cagatcctcc tggcaacttg cacaagtccg acaaccctga
    37981 acggccaggc gtcttcgctc atctatggga tcgccacact cacaacaatg agtggcagat
    38041 atagcctggt ggttcaggcg gcgcattttt attgctgtgt tgcgctgtaa ttcttcaatt
    38101 tctgatgctg aatcaatgat gtctgccatc ttccattaat ccctgaattg ttggttaata
    38161 cgcttgaggg tgaatgcgaa taataaaaaa ggagcctgta gctccctgat gattttgctt
    38221 ttcatgttca ccgttcctta aagacgccgt ttaacatgcc gattgccaga cttaagtgag
    38281 tcggtgtgaa tcccatcagc gttaccgttt cgcggtgctt cttcagtacg ctacggcaaa
    38341 tgtcatcgac gtttttatcc ggaaacggct gtctggcttt tttgatttca gaattagcct
    38401 gacgggcaat actgcgaagg gcgttttctt gctgaggtgt cattgaacaa gtcccatgtc
    38461 ggcaagcata agcacacaga atatgaagcc cgctgccaga aaaatgcatt cagtggttgt
    38521 cataccaggt ctctctcatc tgcttctgct ttcgccacca tcatttccag cttttgcgaa
    38581 agggatgtgg ctaacgtatg aaattcttcg tctgtttcta ctggtattgg cacaaacctg
    38641 actccaattt gagcaaggct atgtgccatc tcaatactcg ttcttaactc aacaggagat
    38701 gctttgtgca tatcgcctcc cgtttattat ttatctcctc agccagccgc tgggctttca
    38761 gcggatttcg gataacagaa aggccgggaa atacccagcc tcgcttcgta acggagtaga
    38821 cgaaagtgat cgtgcctacg cggatattat cgtgaggatg cttcatcgcc attgctcccc
    38881 aaatacaaaa ccaatttcag ccagtgcctc gtccattttt tcgatgaact ccggcaccat
    38941 ctcgtcaaaa cccgccatgt acttttcatc ccgctcaacc acgacataat gcaggccttc
    39001 acgcttcata cgcgggtcat agttggcaaa gtaccaggca tcttttcgcg tcacccacat
    39061 gctgtactgc acctgggcca tgtaagccga ctttatggcc tcgaaaccac cgagcctgaa
    39121 cttcatgaaa tcccgggagg taaacgggca tttcagctca aggccgttgc cgtcactgca
    39181 taaaccatcg ggagagcagg cggtgcgcat actttcgtcg cgatagatga tcggggattc
    39241 agtaacattc acgccggaag tgaattcaaa cagggttctg gcgtcgttct cgtactgttt
    39301 tccccaggcc agcgccttag cattaacttc cggagccaca ccggtgcaaa cctcagccag
    39361 cagggtgtgg aagtaggaca ttttcatgtc aggccacttc tttcctgagc ggggctttgc
    39421 tatcacgttg tgaacttctg aagcggtgat gacgccgagc cgtaatttgt gccatgcatc
    39481 atccccctgt tcgacagctc tcacgtcgat cccggtacgc tgcaggataa tgtccggtgt
    39541 catgctgcca ccttctgctc agtggctttc tgtttcagga atccaagagc tttcactgct
    39601 tcggcctgtg tcagttctga cgatgcgcga atgtcgcggc gaaatatctg ggaacagagc
    39661 ggcaataagt cgtcatccca tgttttatcc agggcgatta gcagagtgtt aatctcctgc
    39721 atggtttcat cgttaaccgg agtgatgtcg cgttccggct ggcgttctgc agtgtatgca
    39781 gtattttcga caatgcgctc ggcttcatcc ttgtcataga taccagcaaa tccgaaggcc
    39841 agacgggcac actgaatcat agctttatgc cgtaacatcc gtttgggatg cgactgccac
    39901 ggccccgtga tttctctgcc ttcgcgggtt ttgaatggtt cgcggcggca ttcatccatc
    39961 cattcggtaa cgcagatcgg atgattacgg tccttgcggt aaatccggca tgtacaggat
    40021 tcattgtcct gctcaaagtc catgccatca aactgctggt tttcattgat gatgcgggac
    40081 cagccatcaa cgcccaccac cggaacgatg ccgttctgct tatcagggaa ggcgtaaatt
    40141 tctttcgtcc acggattaag gccgtactgg ttggcgacga tcaacaatgc gatgaactgc
    40201 gcatcgctgg catgaaagcg cgatggggag acagggcggc atgagacgac agcgacgaag
    40261 tttcaccgac atcatctgcg aaaactgcaa ataccttcca acgaaacgct ccagaaataa
    40321 acgcaagcca atcccaaaag aatctgacgt aaaaaccttc aactacacgg ctcacctgtg
    40381 ggatatccgg tggctaagac attgtgcgag gaaaacaagg tgattgacca aaatcgaagt
    40441 tacgaacaag aaagcgtcga gcgggcttta acgtgcgcta actgcggtca gaagctgcat
    40501 gtgctggaag ttcacgtgtg tgagcactgc tgcgcagagc tgatgagcga ttcgaatagc
    40561 tcgatgcacg aggaaaaaga tgatggctaa accagcgcga agacgatgta aaaacgatga
    40621 atgtcgggaa tggtttcacc ctgcattcgc taatcagtgg tggtgctctc cagagtgtgg
    40681 aaccaagata gcactcgaac gacgaagcaa agaacgcgaa aaagcggaaa aggcagaaaa
    40741 ggcagcagag aagaaacgac gacgagagga gcagaaacag aaagataaac ttaagattca
    40801 aaaactcgcc ttaaagcccc gcagttactg gattaaacaa gcccaacaag ccgtacacgc
    40861 cttcatcaga gaaagagacc gcgacttacc atgtatctcg tgcggaacgc tcacgtctgc
    40921 tcagtgggat gccggacatt accggacaac tgctgcggca cctcaactcc gatttgatga
    40981 acgcaatatt cacaagcaat gcgtggtgtg caaccagcac aaaagcggaa atctcgttcc
    41041 gtatcgcgtc gaactgatta gccgcatcgg gcaggaagca gtagaggaaa tcgaatcaaa
    41101 ccataaccgc tatcgctgga ctgtcgaaga gtgcagggcc atcaaggcgg agtatcaaca
    41161 gaaacttaaa aaactgcgaa acagcagaag tgaggttgca tgaatatcta cgaaagaatt
    41221 gatggcagca aataccgaaa tatttgggta gttggcgatc tgcacggatg ctacacgaac
    41281 ctgatgaaaa aactggagac gataggattc gacaccaaaa aagacctgct tatctcggtg
    41341 ggcgatttgg ttgatcgcgg tacagagaac gtagaatgcc tggaattaat cacattcccc
    41401 tggttcagag ctgtacgtgg aaaccatgag caaatgatga ttgatggctt atcagagcgt
    41461 ggaaacgtca atcactggct gcttaatggc ggtggctggt tctttaatct cgattacgac
    41521 aaagaaattc tggctaaagc tcttgcccat aaagcagatg aacttccgtt aatcatcgaa
    41581 ctggtgagta aaggaaaaaa atatgtcatc tgccacgccg attatccttg tgataaatac
    41641 gagtttggaa agccagttga tcatcagcag gtaatctgga accgcgaacg aatcagcaac
    41701 tcacaagacg ggatcgtgaa agaaatcaaa ggcgcggaca cgttcatctt tggtcatacg
    41761 ccagcagtga aaccactcaa atttgccaac cagatgtata tcgatactgg cgcagtgttc
    41821 tgcggaaacc tcacattgat tcaggtacag ggagaaggcg catgagactc gaaagcgtag
    41881 ctaaatttca ttcgccaaaa agcccgatga tgagcgactc accacgggcc acggcttctg
    41941 actctctttc cggtactgat gtgatggctg ctatggggat ggcgcaatca caagccggat
    42001 tcggaatggc tgtattctgt ggtaagcacg aactcagcca gaacgacaaa caaaaggcta
    42061 tcaactatct gatgcaattt gcacacaagg tatcggggaa ataccgtggc gtggcaaagc
    42121 ttgaaggaaa tactaaggca aaggtactgc aagtgctcgc aacattcgct tatgcggatt
    42181 attgccgtag tgccgctacg ccgggcgcaa gatgcagaga ttgccacggt acaggccgtg
    42241 cggttgatat agccaaaaca gagcagtggg ggatagttgc tgagaaagag tgcggaagat
    42301 gtaaaggcgt cggttattca agaatgccag caagcgccgc atatcgcgct gtgacgatgc
    42361 taatcccaaa ccttacccaa cccacctggt cacgcactgt taagccgctg tatgacgctc
    42421 tggttgtgca atgccacaag gaagagtcaa tcgcagacaa cattttgaat gcgatcacac
    42481 gttagcgcca tgattgccac ggatggcaac atattaacgg cataatattg actttttgaa
    42541 taactttggg gaaacttgac accaataatg ggcgtttttt acatgtcatt gatgagtctc
    42601 aataacctgc cgccgagtag tttttatgct ctgaattgta tttgtgtagt aaacatgctg
    42661 actgc
//
LOCUS       DAZFDC010000021        42300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFDC010000021 DAZFDC010000000
VERSION     DAZFDC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.021"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            86..1237
                     /gene="yiaY"
                     /locus_tag="ACHJ48_001334"
     CDS             86..1237
                     /gene="yiaY"
                     /locus_tag="ACHJ48_001334"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO4706925.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1367..2662
                     /locus_tag="ACHJ48_001335"
     CDS             1367..2662
                     /locus_tag="ACHJ48_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO4706926.1"
                     /translation="MAIKSPPTLIPLSHLSGEELQAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTVRADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            2770..4308
                     /gene="aldB"
                     /locus_tag="ACHJ48_001336"
     CDS             2770..4308
                     /gene="aldB"
                     /locus_tag="ACHJ48_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO4706927.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAEAWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4527..4670)
                     /locus_tag="ACHJ48_001337"
     CDS             complement(4527..4670)
                     /locus_tag="ACHJ48_001337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706928.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCPLTIE
                     TRD"
     gene            4853..5176
                     /gene="yiaW"
                     /locus_tag="ACHJ48_001338"
     CDS             4853..5176
                     /gene="yiaW"
                     /locus_tag="ACHJ48_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4706929.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            5182..6318
                     /gene="yiaV"
                     /locus_tag="ACHJ48_001339"
     CDS             5182..6318
                     /gene="yiaV"
                     /locus_tag="ACHJ48_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4706930.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQKAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(6315..7289)
                     /gene="yiaU"
                     /locus_tag="ACHJ48_001340"
     CDS             complement(6315..7289)
                     /gene="yiaU"
                     /locus_tag="ACHJ48_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4706931.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTLFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            7413..8153
                     /gene="yiaT"
                     /locus_tag="ACHJ48_001341"
     CDS             7413..8153
                     /gene="yiaT"
                     /locus_tag="ACHJ48_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO4706932.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(8283..10253)
                     /locus_tag="ACHJ48_001342"
     CDS             complement(8283..10253)
                     /locus_tag="ACHJ48_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFO4706933.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQTPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEKADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(10264..11664)
                     /locus_tag="ACHJ48_001343"
     CDS             complement(10264..11664)
                     /locus_tag="ACHJ48_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4706934.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCARINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVALFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTHVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            11890..12705
                     /locus_tag="ACHJ48_001344"
     CDS             11890..12705
                     /locus_tag="ACHJ48_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4706935.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHSARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            12707..12847
                     /locus_tag="ACHJ48_001345"
                     /pseudo
     CDS             12707..12847
                     /locus_tag="ACHJ48_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            12949..13422
                     /gene="ysaA"
                     /locus_tag="ACHJ48_001346"
     CDS             12949..13422
                     /gene="ysaA"
                     /locus_tag="ACHJ48_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4706936.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13574..14827)
                     /gene="avtA"
                     /locus_tag="ACHJ48_001347"
     CDS             complement(13574..14827)
                     /gene="avtA"
                     /locus_tag="ACHJ48_001347"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO4706937.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(15005..17035)
                     /gene="malS"
                     /locus_tag="ACHJ48_001348"
     CDS             complement(15005..17035)
                     /gene="malS"
                     /locus_tag="ACHJ48_001348"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4706938.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17355..18179
                     /gene="bax"
                     /locus_tag="ACHJ48_001349"
     CDS             17355..18179
                     /gene="bax"
                     /locus_tag="ACHJ48_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO4706939.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTSRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQDQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(18287..19465)
                     /gene="xylR"
                     /locus_tag="ACHJ48_001350"
     CDS             complement(18287..19465)
                     /gene="xylR"
                     /locus_tag="ACHJ48_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO4706940.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(19543..20724)
                     /gene="xylH"
                     /locus_tag="ACHJ48_001351"
     CDS             complement(19543..20724)
                     /gene="xylH"
                     /locus_tag="ACHJ48_001351"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO4706941.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(20702..22243)
                     /gene="xylG"
                     /locus_tag="ACHJ48_001352"
     CDS             complement(20702..22243)
                     /gene="xylG"
                     /locus_tag="ACHJ48_001352"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO4706942.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(22321..23313)
                     /gene="xylF"
                     /locus_tag="ACHJ48_001353"
     CDS             complement(22321..23313)
                     /gene="xylF"
                     /locus_tag="ACHJ48_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4706943.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLQPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            23679..25001
                     /gene="xylA"
                     /locus_tag="ACHJ48_001354"
     CDS             23679..25001
                     /gene="xylA"
                     /locus_tag="ACHJ48_001354"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO4706944.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAACMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            25073..26527
                     /gene="xylB"
                     /locus_tag="ACHJ48_001355"
     CDS             25073..26527
                     /gene="xylB"
                     /locus_tag="ACHJ48_001355"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO4706945.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            26696..27037
                     /gene="yiaB"
                     /locus_tag="ACHJ48_001356"
     CDS             26696..27037
                     /gene="yiaB"
                     /locus_tag="ACHJ48_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO4706946.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            27083..27520
                     /gene="yiaA"
                     /locus_tag="ACHJ48_001357"
     CDS             27083..27520
                     /gene="yiaA"
                     /locus_tag="ACHJ48_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO4706947.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(27562..28557)
                     /gene="wecH"
                     /locus_tag="ACHJ48_001358"
     CDS             complement(27562..28557)
                     /gene="wecH"
                     /locus_tag="ACHJ48_001358"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO4706948.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYPYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            28732..29031
                     /gene="ysaB"
                     /locus_tag="ACHJ48_001359"
     CDS             28732..29031
                     /gene="ysaB"
                     /locus_tag="ACHJ48_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO4706949.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            29126..30037
                     /gene="glyQ"
                     /locus_tag="ACHJ48_001360"
     CDS             29126..30037
                     /gene="glyQ"
                     /locus_tag="ACHJ48_001360"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO4706950.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            30047..32116
                     /gene="glyS"
                     /locus_tag="ACHJ48_001361"
     CDS             30047..32116
                     /gene="glyS"
                     /locus_tag="ACHJ48_001361"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO4706951.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            32439..32591
                     /gene="hokA"
                     /locus_tag="ACHJ48_001362"
     CDS             32439..32591
                     /gene="hokA"
                     /locus_tag="ACHJ48_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO4706952.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(32568..32639)
                     /locus_tag="ACHJ48_001363"
     CDS             complement(32568..32639)
                     /locus_tag="ACHJ48_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4706953.1"
                     /translation="MRNAVRKAGITFRRRLLLFQIAG"
     gene            complement(32779..32991)
                     /gene="cspA"
                     /locus_tag="ACHJ48_001364"
     CDS             complement(32779..32991)
                     /gene="cspA"
                     /locus_tag="ACHJ48_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO4706954.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(33272..33562)
                     /gene="yiaG"
                     /locus_tag="ACHJ48_001365"
     CDS             complement(33272..33562)
                     /gene="yiaG"
                     /locus_tag="ACHJ48_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO4706955.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            33996..34706
                     /gene="yiaF"
                     /locus_tag="ACHJ48_001366"
     CDS             33996..34706
                     /gene="yiaF"
                     /locus_tag="ACHJ48_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO4706956.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(34756..35730)
                     /gene="ghrB"
                     /locus_tag="ACHJ48_001367"
     CDS             complement(34756..35730)
                     /gene="ghrB"
                     /locus_tag="ACHJ48_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO4706957.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGIAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(35834..36493)
                     /gene="yiaD"
                     /locus_tag="ACHJ48_001368"
     CDS             complement(35834..36493)
                     /gene="yiaD"
                     /locus_tag="ACHJ48_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO4706958.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            36646..38979
                     /gene="bisC"
                     /locus_tag="ACHJ48_001369"
     CDS             36646..38979
                     /gene="bisC"
                     /locus_tag="ACHJ48_001369"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO4706959.1"
                     /translation="MTNSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFTEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(38948..39388)
                     /gene="yiaC"
                     /locus_tag="ACHJ48_001370"
     CDS             complement(38948..39388)
                     /gene="yiaC"
                     /locus_tag="ACHJ48_001370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO4706960.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKVNYWRECIPLVRDAY
                     LANAKNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(39385..39948)
                     /gene="tag"
                     /locus_tag="ACHJ48_001371"
     CDS             complement(39385..39948)
                     /gene="tag"
                     /locus_tag="ACHJ48_001371"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO4706961.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDRKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            40106..40804
                     /gene="yhjY"
                     /locus_tag="ACHJ48_001372"
     CDS             40106..40804
                     /gene="yhjY"
                     /locus_tag="ACHJ48_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO4706962.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            41033..42235
                     /gene="yhjX"
                     /locus_tag="ACHJ48_001373"
     CDS             41033..42235
                     /gene="yhjX"
                     /locus_tag="ACHJ48_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4706963.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTMTVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVKFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT        10411 a        10306 c        10911 g        10672 t
ORIGIN      
        1 ttgataatta ataataatga tcatgttttc gaatgaaaat cctcagtaag ctgcccaccc
       61 ttttttacac tttcaggagt gtgttatggc atcttcaact ttctttattc cttctgtgaa
      121 tgtcatcggc gctgattcat tgactgatgc aatgaatatg atggcagatt atggatttac
      181 ccgtacctta attgtcactg acagtatgtt aacgaaatta ggtatggcgg gcgatgtgca
      241 aaaagcactg gaagaacgca atatttttag cgttatttat gatggcaccc aacctaaccc
      301 caccacggaa aacgtcgccg caggtttgaa attacttaaa gagaataatt gcgatagcgt
      361 gatctcctta ggcggtggtt ctccacacga ctgcgcaaaa ggtattgcgc tggtggcagc
      421 caatggcggc gatattcgcg attacgaagg cgttgaccgc tctgcaaaac cgcagctgcc
      481 gatgatcgcc atcaatacca cggcgggtac ggcctctgaa atgacccgtt tctgcatcat
      541 cactgacgaa gcgcgtcata tcaaaatggc gattgttgat aaacatgtca ctccgctgct
      601 ttctgtcaat gactcctctc tgatgattgg tatgccgaag tcactgaccg ccgcaacggg
      661 tatggatgcc ttaacgcacg ctatcgaagc gtatgtttct attgccgcca cgccgatcac
      721 tgacgcttgt gcactgaaag ccgtgaccat gattgccgaa aacctgccgt tagccgttga
      781 agatggcagt aatgcgaaag cgcgtgaagc aatggcttat gcccagttcc tcgccggtat
      841 ggcgttcaat aatgcttctc tgggttatgt tcatgcgatg gcgcaccagc tgggcggttt
      901 ctacaacctg ccacacggtg tatgtaacgc cgttctgctg ccgcatgttc aggtattcaa
      961 cagcaaagtc gccgctgcac gtctgcgtga ctgtgccgct gcaatgggcg tgaacgtgac
     1021 aggtaaaaac gacgcggaag gtgctgaagc ctgcattaac gccatccgtg aactggcgaa
     1081 gaaagtggat atcccggcag gcctacgcga cctgaacgtg aaagaagaag atttcgcggt
     1141 tctggcggct aatgccctga aagatgcctg tggctttact aacccgatcc aggcaactca
     1201 cgaagaaatt gtggcgattt atcgcgcagc gatgtaatca tcatttccac aacggctggc
     1261 aaattgtcag ccattttttc aactatctca aaacttgctt tttttgagat agtttctctt
     1321 tcctgctata cactatctca atattgcgct tttttgagat aagcaaatgg ctatcaaatc
     1381 acctcccacg ctgattcctt tatcacactt atcgggtgag gaactccagg cccacctgcg
     1441 ctttaaccgg gttaccgatg aaaaaggacg ttatctgcct tttgatgagt tgcagtaccg
     1501 catcaaaaaa ggagaaaacg tcgatgtggc gtggacgctt acccgtctgg cgcgcaacgc
     1561 ggcgatccaa cgaattaatt attgcaatga agcgggtgaa caggccggat tcaacattac
     1621 cccggtgatt gcagaagcgt gcgagcttgt cgacaaacgt gctaccgcgc tggcgctgaa
     1681 agatcagact gaacggttac gcggggccgg agccgagtta agccagttac gcctggaaga
     1741 gccgatcacc agctcccagc tggaaggtgc gaatactaca acgctggtgg cgagaaaaat
     1801 gctggaaaca ggacggtcgc cacgcactga agacgagcat atgattgcgg gaaatgcccg
     1861 gctgatggcg gaaattccgc acttactcgc agaaccgctg acacctgcgc ttattcgcca
     1921 gttacatgcc attggtatgg gtggaatcaa tgacgcgaaa tatcgtccgg gagaatttcg
     1981 tgaaacagat gatgttgtga tcgccgacta tgatggcaat atagtgcatc aaccgcccgc
     2041 tgcggcattg ttaccggaac ggctggaaaa agtttgccag tggttaaaca gccatgaagg
     2101 gtatattcat ccgttggtcc gtgcctgcat tctgcatttt atgctggccc atgaacatcc
     2161 gtttcgggat ggcaacgggc gcaccagtcg ggcgctgttt tactggtata tgctgaaatc
     2221 gggctacgat gtctttaaat atatttccat cagccgtttg cttcatgctg caccggtcaa
     2281 atatgccgcc agctaccagt acactgaatc agatggtatg gatctgactt atttccttga
     2341 atatcaggcg ggtgtcatta aacgtgctct gcaaaactgg cagcagcata tcgatgaaat
     2401 tacacaacga agcgcaaaac tcgatagtgt gctgtttagc tctggtgtgc ttaaacgcct
     2461 gaatccgcgc caggtaacgc tcctgaatgt gatgctcgcc aatccgggga aagagtacac
     2521 agttgctgaa attagcgcga gcctgggcgt ctctgataat acggttcgag ctgatttacg
     2581 gacaattgtt aaagaagggt ttgcgcaaga gaagaaaatt aatgaccagc aagccgtgta
     2641 cttcgcgcat tatccgctgt aacccttgcc cgtaaattcg tgatagccgt cgaaaagctg
     2701 ttaccgactg gcgaagattt cgccagccac gtctaccctt gttatacctc acaccgcaag
     2761 gagacgatca tgaccaataa tcccccttca gcacagatta agcccggcga gtatggtttc
     2821 cccctcaagt taaaaaaccg ctatgacaac tttattggcg gcgaatgggt agcccctgcc
     2881 gatggtgagt attaccagaa cctgacgccg gtgaccgggc agctgctgtg cgaagtggcg
     2941 tcttcgggca aacgagacat cgatctggcg ctggatgctg cgcacaaagt gaaagataaa
     3001 tgggcgcaca cctcggtgca ggatcgcgcg gcgattctgt ttaagattgc cgatcgaatg
     3061 gaacaaaacc tcgagctgtt agcgacagct gaagcctggg ataacggcaa acccattcgc
     3121 gaaaccagtg ctgcggatgt accgctggcg attgaccatt tccgctattt cgcctcgtgt
     3181 attcgggcgc aggaaggtgg gatcagtgaa gttgatagcg aaaccgtggc ctatcatttc
     3241 catgaaccgt taggcgtggt ggggcagatt atcccgtgga acttcccgct gctgatggcg
     3301 agctggaaaa tggctcccgc gctggcggcg ggcaactgtg tggtgctgaa acccgcacgt
     3361 cttaccccgc tttctgtact gctgctaatg gaaatcgtcg gtgatttact gccgccgggc
     3421 gtggtgaacg tggtcaacgg tgcaggtggg gaaattggcg aatatctggc gacctcgaaa
     3481 cgcatcgcca aagtggcgtt taccggctca acggaagtgg gtcaacaaat tatgcaatac
     3541 gccacgcaaa acattattcc ggtgacgctg gagctgggcg gtaagtcgcc aaatatcttc
     3601 tttgctgatg tgatggatga agaagatgcc tttttcgata aagcgctgga aggctttgca
     3661 ctgtttgcct ttaaccaggg cgaagtttgc acctgtccga gtcgtgcctt agtgcaggag
     3721 tcaatctacg aacgctttat ggaacgcgcc atccgccgtg tcgaaagcat tcgcagcggt
     3781 aacccactcg acagcgtgac gcaaatgggt gcgcaggttt ctcacgggca actggaaacc
     3841 atcctcaact acattgatat cggtaaaaaa gagggcgctg atgtgctcac cggcgggcgg
     3901 cgcaagctgc tggaaggtga actgaaagac ggctactacc tcgaaccgac gattctgttt
     3961 ggtcagaaca atatgcgcgt gttccaggag gagatttttg gcccggtgct ggcggtgact
     4021 accttcaaaa cgatggaaga agcgctggag ctggcgaacg atacgcaata tggcctgggc
     4081 gcgggcgtct ggagccgcaa cggtaatctg gcctataaga tggggcgcgg catacaggct
     4141 gggcgcgtgt ggaccaactg ttatcatgct tacccggcac atgcggcgtt tggtggctac
     4201 aaacaatcag gtatcggtcg cgaaacccac aagatgatgc tggagcatta ccagcaaacc
     4261 aagtgcctgc tagtgagcta ctcagataaa ccgttggggc tgttctgatg taagaagctg
     4321 gtcgcatcgg gtattaattg cctgatgcga cgcttacgcg tcttatcatg cctatgggga
     4381 cctgaccgta ggccggataa ggcgtacacg ccgcatccgg cataaacaaa gcgcacgttg
     4441 ttacaattta cgttttaaac ctacgtgtgc aataacccag actacgccaa caaaagtgcg
     4501 tttctggggc gtgttttgcc aggagtttaa tctcgcgttt cgatggtgag cgggcaaacc
     4561 agaccgtaaa tcaccattgg tattccgagg acaatcagca ataaacggta gattattgaa
     4621 ggtttaatac gcttaatctg cattgtggat gttttactga agttattcat tagtttggct
     4681 gatagttcca ttatttcaat atgataagcc tcgacctgtt aaaaagccac gcagcacact
     4741 tcagaaagta tataaaaaac aatatgtcgt tatataaata aggaaattgc attgcatcat
     4801 ggcatttcat aatatgcgtt aattgttctt ttttatattc tctgagggaa atatgttcct
     4861 ggactatttt gcactgggag tgcttatttt tgtatttctg gtgatattct atgggattat
     4921 tattttacat gatattccct acctgattgc taaaaaacgt aatcatcctc atgccgacgc
     4981 cattcatgtt gctggttggg tgagtctttt tacgctgcat gttatctggc catttttgtg
     5041 gatttgggcc acgctttacc gcccggagcg gggatgggga atgcaaagcc atgattcatc
     5101 cgttatgcaa ctgcaacagc gcattgccgg gctggagaaa cagctcgccg acatcaaatc
     5161 ctcttctgcc gagtaatact tatggattta ctgattattt tgacctatgt ggcttttgca
     5221 tgggcaatgt ttaagatctt caaaattccc gtaaataaat ggaccattcc cacagcggcc
     5281 ctgggtggaa tatttattgt cagcggtcta attctgttaa tgaactataa ccatccgtat
     5341 acctttaaag cgcaaaaagc ggttatttct attcctgttg tcccacaggt gacaggcgtg
     5401 gtgatagaag tgacggataa gaaaaatacg ctgattaaaa aaggtgaggt gctatttcga
     5461 ctggacccga cgcgttatca ggcgcgggtg gatcggctga tggcggatat cgttaccgca
     5521 gaacataaac agcgggcgtt gggcgcagag ttagatgaga tggcggcgaa tactcagaag
     5581 gcaaaggcca cgcgggataa attcgctaag gagtaccagc gttacgcacg cggcagccag
     5641 gcgaaagtaa acccgttttc agaacgcgat attgatgtgg cacggcaaaa ttatctggcg
     5701 caggaagcct ccgtaaagtc atcggcggcg gaacaaaaac agatccagag ccagctggat
     5761 agcctggtgt tgggtgaaca ttctcaaatc gccagcctga aagcgcagct cgcggaagca
     5821 aaatataacc ttgagcagac gatagtgcgt gcgccgagcg atggttatgt gacccaagtg
     5881 ctgattcgtc cgggtaccta tgccgcgtcg ctgccgctac gtccggtgat ggtgtttata
     5941 cccgatcaga aacgacaaat cgtggcgcag ttccgtcaga actccttgct gcgcctggct
     6001 cccggcgatg atgcggaagt ggtatttaac gctctgccag gcaaagtgtt cagcggtaag
     6061 ctggcagcca ttagtccagc tgttcccggc ggagcttatc agtcgaccgg caccttacag
     6121 acgttaaaca cagcgccggg ttcagatggc gttatcgcga ccattgaact gaatgagcac
     6181 actgatttga gcgctttacc agacggtatt tacgctcagg tggcggtcta ctctgatcat
     6241 ttcagccatg tctcggtgat gcgcaaagtg ttattacgca tgaccagctg ggtgcattac
     6301 ctttatctcg atcattaatc ttcgctttcc gccgcttgtt caggccgcag ttgctgcgac
     6361 tgagcaagct ggtggcgaat ttcacgcagc atttcatcca gcacatactg gacccgccag
     6421 ggctggtatt cccgtttacg gaagatagcc accagatcca gccaccattc atacgggtgc
     6481 tgaaagcagg gcaccagcgt gccgtcttgc agatcgctct gcacactttt gtctggcgca
     6541 aacacaatcc ccaggtgatt tcgtgccagc tccagcgccg attgcgtgtt atcgcaaacg
     6601 taatttccct tcacgcgata atcccgcacc tctttactcc cggcgacgtt gaagcgccag
     6661 atattcgcgt cgtcgatcat catcgagtcg atcaaaatac aggagtgatg ttcgagttcg
     6721 tcaggacggc taataggatg tttctcaaga tagcgctggc tggcataagc ggttacggca
     6781 tattgggtaa taaaactggc aaccagcgat tcatcttttg gtggggcata gctgattaaa
     6841 acatcacaat catcaggaaa ttcgacgcct tcataaaagg cattacgctc aagattgcag
     6901 gtttttagcg ataaggtgat atcaccgata tctttaattt tatcgattac atgtttggat
     6961 aaataggtaa ttatgccggt tggggcgtag atggtgaccc gaccacgttt ctcatgctta
     7021 taatctgcaa taaaattatt aagctgctcg tttctgtcca gcatgtcgtt gatgtacggt
     7081 aatagcgcgg tgccaaaaag tgtgagcatc agttgtcggg tggttcggtc aaagactttt
     7141 aaacccactt ttgattcaaa atcagcaaga tatttgctga cgttggcctg tgcgatgcca
     7201 agtacagtcg cggcatggct gatattttca ctggcagcga ttaccgagat aatttttaac
     7261 tcccggtact taagttgtaa ttttgtcatc gcaacagccc aattatatat aattttatat
     7321 attactatat aaatatgaat cttgcaccgg agaaattgta agcattacta tgccgactct
     7381 tttttatctt attttaaaat ggaataccgg acatgttaat taaccgcaat attgtggcgt
     7441 tatttgcgtt gccttttatg gcaagcgcaa ctgcttctga attatccatt ggtgctggtg
     7501 cggcttataa tgaatcgcct tatcgcggtt ataatgaaaa tacaaaggca attccgctga
     7561 ttagttatga aggtgattct ttttatgttc gtcagaccac gttaggtttt attctgtcgc
     7621 aaagtgaaaa aaatgaactt agcctgaccg catcctggat gccgctggaa tttgaccctg
     7681 ccgataatga cgattatgcc atgcaacagc ttgataagcg tgatagtacg gctatggcgg
     7741 gggttgcctg gtatcaccac gagcgttggg gaaccgtgaa agcctctgca gctgcggacg
     7801 ttctggataa cagcaacggc tgggtggggg agctatcggt attccacaaa atgcagatag
     7861 gtcgtctgtc gctgacacct gcgctgggcg ttctctatta tgacgagaat ttcagtgact
     7921 attactatgg catttcagag agtgagtccc gtcgtagcgg tctggcaagt tattccgcgc
     7981 aggatgcctg ggtgccctat gtcagcctga cggcaaaata cccgatagga gagcacgtcg
     8041 tattgatggc gagcgcagga tacagcgagc tgccggaaga gattaccgac agcccgatga
     8101 ttgatcgtaa tgagagtttt acctttgtca ccggcgtgag ctggcgtttt taattcaccg
     8161 gtggatgtcg gtgcggctgg cctacgctct cgcaccgact tgtatgcctg ataagacgtg
     8221 ttaagcgtcg catcaggcaa tgtgctcctt acgttggatg cggcgcgagt gccttatcca
     8281 acctacggat ggcaatgctt ttcctcattc acccagatcc gcatttcgcc ttcaccccgg
     8341 ttggcccagc taaaccacgg gataaaggtc agagtttgcg tctggcgttt ggctggcgcg
     8401 ctgtcgtaat gccacagcgg ttgctgctct ggattgctct gttcataccg gtaacccggt
     8461 gcctggatta agatcttatg gctaaacaat cccttgcctt caaatgtcgt aaatggcgca
     8521 tcggtgggca gccacagatt atgcagtgac tcgccgttgt cggccttttc caggcaatac
     8581 accagcgggc cgcgctgaat cgccactttt ccggcgacgt gacgcaccag cgggttaccg
     8641 taaacgcggc gtaccggcat cggcaaagtc agattcagcg tatcgccctc ctgccattcg
     8701 cgggtaatgt gcaaataccc tttacgaata tcctgctcga cctcttcccc attcaatatg
     8761 atctgcggct gtgtgcacca gtccggcaga cgtaaagcca gcgtatgacg taccggctgg
     8821 ggcgattcaa ccgcaatcgt cacctgctcc tgccacggat agttcccgct aacccgcagg
     8881 cgcagcgtgc cattttctac cggcacttcc atgctgtttc ctgcgtagat gttgatatac
     8941 aacgcatctt cacgcggcgt gtagagataa tgaccaatcg aggtcagcac gcgggcgata
     9001 tttggcggac aacaagcgca gccaaaccaa cgctggcgga tcggtttaac gtgatcgtag
     9061 atatggttga atttcagcga ttttggatgt acttccagtg gattcacata gaagaaatgt
     9121 ttgccatcca atgccatgcc gccgagcaca gtgttgtaca gtgcgcgctc catcacatcg
     9181 gcatattgac tgtcgccttc catttccagc attcgtcggg cgaacatcat caggccgatg
     9241 gaagcacaac tttcggcgta aaccgtgtca ttcggcagat cgtaatcgct gctgaacgct
     9301 tcgccgctgc tttgtgagcc gatgccgccg gtaatatata actgacgctg ggccatattg
     9361 ttccacagcc gcaggcagtc ctgacgcttg ctgtcatcgt gacttaaacg cgcgagatgc
     9421 gcgacgccgg tcatcagata gacaaaacgt accgcgtgac cgatggcggt ttgctgttgc
     9481 gcaaggggca aatgtgcctg gctgtaggct ttgtctttca ccatccacgc cgggccgtag
     9541 gtgtgccagt gcgatgtctg cccgcgcttt tcatattcct ggtcgtaata gtgcggttgc
     9601 gcaccacgct gttcgacaaa atagttcgtc agcgccaggt agcgcggctc ttcagtcact
     9661 tcatacaggc gcattagtgc cagttcaatt tccgggtgac caggataacc gtgtaactta
     9721 ctttcatctg gaccaaatac gctgtcgata tgatcggcca ggcggcaaac gacccccagc
     9781 aagcgccgtt ttcccgtggc ctggaagaag gcgactccgg cttcaatcag atgacctgcg
     9841 cagtaaagtt catgacactc cgccagattg ctccagcgtt cttcgggtgc ttttaccgta
     9901 aagtaagtat tgagatagcc gtcttcgcat tgggcggagg cgatcagttc gattacctcg
     9961 tcggcggttt tttccagttc ggcgtccggc gtctggcaca gcgaccaggc taccgcttcc
    10021 agccatttgg cgacgtcgct gtcctgaaac accatcccgt aaaattcacc ctcctgaagt
    10081 ccggcggcaa tgcgaaagtt ttcaatcgca tggctgggtt ccgcttctgg gatacgatcg
    10141 ttcaaggcat cccactgata aggaatcacc acgtcgcgga ccagttgttg gtactgaccg
    10201 aggaacggat cgctgaccgt cagtttatgc agatcgactt ccgaaatgtt catcttgtgc
    10261 tccttaaggc tgtacatgac gctgacgcag gtcggtagct atgcgtgaca tcataggatt
    10321 attgagtttg caaaagcgga tcgcccctgc cagcagcagg tggaatagcg caggcagtaa
    10381 tgtttccatc gccgcgatcc cgccaagcga actggcggtc tggttttcca cgccaggctg
    10441 gtaggcgaca aaaataaaca gcaggctgat gatcccggcg ctggaggccc aggccagctt
    10501 gatgaaaaac agattgaagg cgaagttcat gccggaagaa cgtacgtggg ttttccactc
    10561 gccgtagtca tcggcaaagg ccatcaatga gaagtgcagt ggcaatgtga agccaagaat
    10621 cacgccatta ccgaggatca ccgccagcca cagcgtttga taagccgggc cggagggcag
    10681 gaaccacatc aacaccgcca gtgcagcgag gagcaggttg gtgtagtaat aaattttgac
    10741 ggtatcgaaa cgccgcgtta acgggctgac aatcaccgag ccgataatgg aggcgaaggt
    10801 gaccatggtg aagaacagcg acgtgtagcc cgtgctgcct tgtaagacgt aggtaatgaa
    10861 gtacatatac ccaccgccgc gaatgttaaa gacgttaatc agcaggaaag acatgaccag
    10921 catcagcagc agttggtcgt tgttccgcag cccggcaaga tgttcgcgca gggtaaattt
    10981 ccccattgtg gagagcggca cccgttcacg aacccagaag aaacagcaca ggaacatcac
    11041 cacggcaatg gcgcacaata ccccgacgcc cagttgatag ccgcgtgcag cgttgccctg
    11101 accgaagaga gctaccatcc acggtaagcc taccgaaacc agaaatcccg ctacgccgca
    11161 gagaacgaat cgccaggact ggcaggagat cacttcattg tggcgggtgg tcatggtgtt
    11221 gatccgcgcg cagtacggta cgttgatggc ggtataaccg actgaaagga gcaggtaagt
    11281 accgaacgcc caggcgattt ttactcccat gctggcatct ggcacggtga acgtcaggat
    11341 gccgataatg ccgatgggaa tggcaatcca tagctgccac ggacgaaagc gcccccatcg
    11401 gctttgcgtc cggtcggcaa tgacgcccat caccgggtcg gagatggcat caaacacgcg
    11461 cagtgcaata aacagcgtgc caaccagcgc cggagttaat ccaaaaacgt cggtgtagaa
    11521 aaaggtgaga aaattcatga tcaggcaagt tattacggtg ccgcctgcgt cacccaggcc
    11581 gtagcctatt ttttcccgta cagataacgc atcgtcaatt ttacctttag cgatatcagc
    11641 ggtagtaatc ggtgtagagg tcatagatat tcctcgtgaa tagggagcaa tttttagttt
    11701 tattgaaatg acctgattaa ttctgccgtg aacgggaata tcaacaaggc gagaaaagag
    11761 taaaaaaagg tgacgattcc gacctgaatt taaaagtgtg aaatggatca ggtgaaatta
    11821 atgtttttga ttaataatca aaaggtaatg tgtttaatca ttaatgaata agcgggaaat
    11881 tacatatcaa tgctcgaatt atccatgacg ttaccgatta aagtgcaaaa tggtggatta
    11941 tttatttccc gtggcgtagg gaggcattct gcaaggcggc tggagtcatg ggaaattatc
    12001 tttgtcgaaa aaggtcgatt aacgattcag gaagaagaac ggatatttct ggtcgatgcg
    12061 ggagagagtt tattactctg gccgaaccga caacatgttg gggtggagga ttttcccgcc
    12121 gatctcaaat tttactggct gcattttgag gttaaagcgc cggacaacga ccctcgctgg
    12181 cttactcatc ttagcgtgcc gcaacaaacg aaggttgtcg atccgcaggc gctggtggcc
    12241 ctgtttcgcc agttcttaag cgaacaggaa aagcatcaac gcactccggc gctggagttt
    12301 attgtgctgc tcattttgca gcagctcacc cgtgacgcgc gtcttgatga acaggccgat
    12361 ggcgcaggca ccacccttgc ctggaaagcg cagaaactga tccgcacgca atatcatcgc
    12421 ccgctttcta cggcgcttct ggcccgcgag ctgtactgca atgccgatta tcttgggcgc
    12481 gtctaccggc gggtgtttca tctgacgtta acagaagcaa tccatcgcca gcgggtgcgc
    12541 gaagccgaaa agctgttaat tagcgaagcg cgatcgctca cggaagtggc ggcgttgtgc
    12601 gggtttaatg acgttggcta tttccggcag atttttcgca aacacaccgg tttaacgcca
    12661 gcagcgtgga agcggcgtta cagcaaggaa catatcaatt cgtagtgccg gggcgatttc
    12721 gctttcgaca tcacgtctga aacaggtggg ggagaaaaat ctcctgaaac cactgcgtga
    12781 aaccgcgcag gctatttcta atgaactggg atttactgtc agggacgacc agggcgcgat
    12841 cacataacgc tttggacaag tgccaaaact ttaacatttc cttcgttgga tcaaagcagt
    12901 agggacgcgc tctctggcac tctgctgttt tagtgcaaag gagtgatcat gaaccggttt
    12961 attattgcgg atgcgacgaa atgtatcggt tgccgtacct gtgaagtggc ttgcgcagtg
    13021 tcgcatcagg agaatcagga ttgcgctgcg ttgtcaccag acgagtttat ttcccgtatt
    13081 cgtgtcatta aagaccacag ctggaccacg gcagtagcct gtcatcagtg tgaagatgca
    13141 ccgtgcgcga atgtctgccc tgttgacgcg ataagccgcg aacatgggca tattttcgtt
    13201 gaacaaacac gttgcattgg ctgtaaaagc tgtatgctgg cttgcccgtt tggtgcgatg
    13261 gaggtcgttt cttcgcgcaa aaaggcgagg gcgattaagt gcgatctgtg ctggcatcgg
    13321 gagacgggac cggcctgtgt tgaagcctgc ccgacaaagg cgttgcagtg catggatgtc
    13381 gagaaagtgc agcggcatcg gctacggcag cagcctgttt gaaacgtttt tccggcgttt
    13441 ctcaacgccg gaatagtgcg ctttgtttat gccagatgcg gcgtaaacgc cttatccggc
    13501 ccacaaattc ttgcaaatta aatatattgc agaaatcatg taggcctgat aagcgtagcg
    13561 catcaggcaa tttttagtga ctttcagccc aggctctttc tatctcttcc gccagaatct
    13621 tcacccccgc ctcaattttc tccggctctg gtacgtagtt catgcgcata cattgatgcg
    13681 tatgcggcca cggtttgtcc agccctggga agaagttgtg ccccggcacc atcagcacgc
    13741 cgcgtgcttt caggcgctga tagagctgct cggtcgtaat gggcaaatcc ttaaaccaaa
    13801 gccagaggaa aatggctcct tctggtttat gaattaggca gcgatcttcc ggtaaatagc
    13861 ggcgaatgat ggcgatagtt tcctgaacac gctggtagta aaacggtttg atgactgttt
    13921 cagacaggcg cagcagatcg ttacgcttaa tcatttcaca catcatcgcc ggaccaatac
    13981 cgccaggtgc caggctgata atgccgttca tattggtgat ggcggtgatg attttttcat
    14041 tggcgatgat aatgccgcag cgggagccag gcagccccag cttggaaaga ctcatgcaca
    14101 gcacgatatt cggattccat agcgggcgcg cttcactgaa gatgataccc gggaacggga
    14161 cgccataagc gttatcaatc accagcggaa tgccgtgttg attcgccagc gcgtcaagct
    14221 tcagcaactc ttcgtcagta atcacattgc ctgttggatt cgtcggccgg gagacgcaaa
    14281 tcatcccggt ttcttcgcca atatgcagat gctcaaaatc gacgtggtat ttaaactggc
    14341 cttccggcag cagttcaata ttcggacgcg cagagacaaa cagatcttct tccagtccgg
    14401 cgtcagcgta gccaatgtat tccggtgcca gcgggaacag cacttttttg acccgaccat
    14461 cggcacggcg tccggcaaac agattaaata agtagaaaaa cgcgctctgg ctgccgtttg
    14521 ttagtgcaat attctgtgct tcgatatccc aacccaactt ctcgcgcagc attccggcaa
    14581 gcagtgtgag tagctccgtt ttcccctgtg gaccgtcgta attgcacagt gcatcagtcg
    14641 ctttgccact ttccagcatg tcggtcagta gcgtctggaa gtagtcctgc atttccggga
    14701 tctgcgccgg attaccgccg ccgagcataa tcgcgccagg cgtgcgtaaa ccgtcgttca
    14761 gatcttccat cagcagcgta atgccggagt ggcgggtaaa tttgtcacca aaaagggaga
    14821 atgtcatagc gggatatctg tcgaactctg tagcaaggaa ggtaacaata acgctacatt
    14881 tgctggggtg caaatcggtc tgttgaagag tgagcggtgc tttatcctgc aacgctgatt
    14941 agggctgaca ttttatcctg gttggcggct tcccgaggta ctgacgaacg ggaagccgga
    15001 aaagtcactg ttgcccagcc cagatgacca gcactttatc gtcgccatgc tcacgaacaa
    15061 agccgtagcc ctgcttcagc gaaagtgtcg tttgtttgcc cgcgccaatt gcgggatggc
    15121 gggcgcggaa ctggctgatt ttctgccagt gcgcgacgtt ggcggcagat ttaccgctaa
    15181 catcctgcca gttcatatcc gaacgtgtac cttgcagcgg atcagaacct gtaggaccga
    15241 acggacgcga ggattcatcg ccataaaaga tttgtaccgc gcctggcgct aatagtaata
    15301 actctgctgc tttgtcgccc ccttcacgga acagacgggt atcatgcgac gagaggtagc
    15361 tcaacacgtt gaaaccctgc aatttctccg ccatttgctg ccaggtcgta tccatctgcg
    15421 caatacaatc gacagctttc gccgcctgct cctgataatc gaaattgatc atcgcatcga
    15481 agccgtggcg atagtagtca ctttgcatca cgccgtggcc ccaggcttca ccggtcatcc
    15541 agaaaggttt gtcatctaat gctttgtcgg ggttagcttt tttccattcg cgaagcgcgg
    15601 cgctggcttc ggttttcagt tgctgccaag cgggcaactc aacatgtttg gcggtatcga
    15661 cccgaaaacc atcaatccca tagtcgcgga cccactgact taaccagtgg gttaagtaat
    15721 cgcgaggggt aaagccgtcg atggctttag cgtgggtatc cgttttgttt ttatagaaca
    15781 ccggcagacc agaagcggta gttgattcgg ttttgatatc cggcaaaaag gctagcgaca
    15841 tggtgagatc gtcgaatcca ggattgtcgt aatcgccgat atcggtacgg atccagtttt
    15901 ttccccacca tttatcccag cctgttttgt cgctgaaatt aatgtaatcg ttaaagctat
    15961 gccaggtttg cccggcggca ggtttccagt cgctccagcg ttcacccagc gtttttttca
    16021 cttcgtcacc agaaagatat aacgcgccaa actgatactc ctgcatatcc gccagcgtgg
    16081 catagccggt gtggttcatc acgacatcaa agagaatacg aataccgcgc tgatgtgcgc
    16141 tatcaaccag cgtccgtaga tcggcttcgt tgcccatatt ggcatcaaga ttcgtccagt
    16201 cctgtgtgta ataaccgtgg taggcataat gcgggaaatc gccttttgta ccgccgccga
    16261 cccagccgtg aatttgctca aatggggcgc ttatccataa agcattaacg cccaactgct
    16321 ggaggtaatc cagtttgttg gtcaggccgc gtaaatcgcc gccgtgaaaa gtgccaattt
    16381 ccgccatacc gtctttatga cgtccgtaac tctggtcatt actgggatcg ccgttttcga
    16441 aacgatctgt cagcacaaag taaaccgtgg cgttatgcca gtcgaaaggg gcagaggcgt
    16501 cagtttctgc ccgttccagc aggagtaaac cgttgctggt agcagcgggt tgtaacgtta
    16561 tttgaccgtt cttcactatc gcaatttgct ggctgtaata atcccgtacg gcggctcctt
    16621 ccgggaaagt ggcgctgaca tccagcgtga gcggtaatcc atcccatttc gggcattcac
    16681 ggaccaggtt tgctaccggt tcggcggcgt tctggatgga aatcatcaat gttggcgtac
    16741 cggagcgggt gtctatttgc agcgtatatt tgccgtccct gaacaatcgc cattgaggcg
    16801 gcgtgttgct acaaggttgc agggaaagca tctgattgag ttttatcgca tctgcaggct
    16861 gccagcactg ctggtcaaaa tttagcgtga gtggacgcgt acctttgggc aactgcgcgt
    16921 ggctgacaaa tgttcccgtt ccctgttcgc taaaggcagg gaaccccgga gaagtccagc
    16981 tggcggcaac ggcgaagcca ggaaggagtg tcagaaaaca ggcggcgagt ttcatggtgg
    17041 atgagtcctt attgctgcga tttcagacag tttgccagcg ataagccaga caaaactcat
    17101 ccctgagcgg taagtccaca ggatgagaag caagggtgag cgatcgcgcg caaaaaacgg
    17161 ctgaattttg cgataaaccc cacattttct gcgatttagc gccaatctga atcgttaact
    17221 cgtgacatag tttcagattt ggactattcc ttggggtgct cttgacagct atttttgata
    17281 tgatttgaga ttcaactctc aaatttgtga aaaatataag gtgttggaat gattaaatcc
    17341 gaccaggaga cctgatgatt ttgactccca tacgacgata tggggcgatg attcttatgt
    17401 tactcactct ggtgttttcg agtgaggtgt tagcgaagac gcacacaaca acagcgagtc
    17461 aaaagtccca cttaactaaa gctagtaata aacaggtaag cagtaaacaa gagtattctc
    17521 gcaatagtgc aaagagtagt tcacttcctg atttgcgaaa atacccttcc ggaacatcaa
    17581 ggaaaaaggc gtttctccgg accgtaatgc cttacattac cagccaaaat gcagccatta
    17641 ctgcagaacg taactggctg atttcaaaac agtatcagga ccaatggtca cctgctgagc
    17701 gtgcgcgtct gaaagacatc gccaaacgct acaaggtgaa gtggtctggt aatacgcgaa
    17761 aaatcccgtg gaataccttg cttgaacgcg tagacattat ccctaccagt atggtggcga
    17821 cgatggctgc agcagaaagc ggttggggaa cgtcgaagct ggcgcgcaac aacaacaatc
    17881 ttttcggcat gaaatgcatg aaaggacgtt gtaccaatgc gccaggtaaa gtgaaagggt
    17941 actcacagtt tagttctgtc aaagaatcgg tgagcgccta tgtcactaac ctcaatacgc
    18001 atccggctta ctcttcgttc cgtaaatcgc gtgcgcagct gcgtaaagct gatcaggaag
    18061 tgactgccac agcgatgatt cacaagctga agggctactc gaccaaaggg aagagttata
    18121 acaactacct gttcgcaatg taccaggata accaacggtt aatcgcggcg catatgtaat
    18181 tgtatttcct cgagccttat ccgacttgtc ggtcggataa ggcttttgat cgcatcaggt
    18241 ggttgtgctg agttacctga tgcgacgccg acaattctca tcatcgctac aacatgacct
    18301 cgctatttac atcgcgatac tcttttggcg tcgtgtcata tgctttttta aaaacagagt
    18361 agaaatattg cagcgatgga taaccgcaca tttgcgatat ctcattgatc gacaaggtgg
    18421 ttgaaatcag cagactgcgc gctttctcca gcttctcagc atgaatcatg gcatggatgg
    18481 tttcacccac ctcttcttta aaacgcttct caagattgga gcgcgagatc ccgaccgcat
    18541 cgagtacctg atccacttta atccctttac aggcgtgatt acgaatgtaa tgcatggcct
    18601 gaataacggc gggatcggtc agcgaacggt aatctgttga gcgccgttca atgacgcgaa
    18661 ctggagggac caaaatccgc tgtagcggca tttcttcttt atctaataat cgatgcaaca
    18721 gttttgccgc ctgatagccc atttgccgcg cgccctgagc gaccgaagaa agggcgacac
    18781 gcgacagata gcgggtcagt tcttcgttat cgatgccaat cacgcataat ttttccggta
    18841 cgggaatgtg tagatgttca catacttgca gaatatgccg cgcccgggcg tcagtaacgg
    18901 caataatccc ggtttgcggt ggcagtgttt gtagccagtc tgccagccga ttttgcgcgt
    18961 gttgccagtt ctctggtgcg gtttctaacc cctgataaac cactccgcga tacttttctt
    19021 cggcgacaag ctgacgaaat gcatattcgc gttcagtggc ccaacgtttg ccgcttgatt
    19081 ccggaagacc ataaaaagca aagcggttaa cgcctttctc ttttaaatgc aaaaatgcgc
    19141 tttcaaccag cgcatagtta tcggtggcaa tgtaatgaac gggtgggtaa ctttctgcaa
    19201 ggtgatacga gccgccaacc ccaacaatgg ggacgtcgac atcagccagc gcttgctcga
    19261 tctgtttgtc gtcgaagtcg gcaatgacgc catctcctaa ccagtccttg attttatcaa
    19321 tgcgggcgcg gaaatcttct tcaatgaaaa tatcccattc cgattgtgac gcctgtaaat
    19381 attcccctac gccttctact acctgccggt cataggcttt attggcattg aacagtaatg
    19441 tgatgcggtg acgtttagta aacatggttc ttttcctgct gaatcatgca aaaactcaaa
    19501 accggtaata cgttaccggc tttgagaaaa tttttatcaa aatcaagaac ggcgtttggt
    19561 tgcggagtcc atccatactg ccagcaacag aatcgcacct ttaacgatat actgccagaa
    19621 ggtcggtaca tccatcatac tcataccgtt atccagtgaa gccatgatga atgcccccat
    19681 taccgctcca gccacgctcc cgacgccgcc tgccagactg gtgccgccaa tcacgcatgc
    19741 tgcaattgcg tccagctcgg cgatattccc cgcagaaggt gaaccagcgc caagtcgaga
    19801 actgaggatt aatccggcga tggctaccat taatccgtta atagcgaaca cggcaagttt
    19861 ggtgcgttca acgttaattc cggagagacg tgctgcttcc agattgccgc cgatggcata
    19921 aatgcgtcgt ccaaatgctg tccgcgttgc cataaacatt ccgccgagta acagcaacgt
    19981 cagcagcaga acaggagtag gaacgccacg gtaatcattc aacagccaga ttgcgcctaa
    20041 tacgatgata gcggttaaag cctggcgacc gactactgcg gtagaggctg gagactgcaa
    20101 acccaaagcc tgacggcgca ttcttccgcg ccattgccag ccaacaaaag ccattaagcc
    20161 aagcgcgcca atgatgaagc cggtgctggc ggggagatag ctttgcccaa tttgtgacat
    20221 cgcggcgctg gtgggggata cagtcgtgcc gttggtgatg ccaatgagta tgccgcgaaa
    20281 tgccaacatg cccgcgagag tgacaataaa tgaagggact ttgcggtacg cgacccacca
    20341 tccgttccag gcaccgagaa gcagtcccag aaccaacgtc acaatgatgg taagtggcaa
    20401 aggccagcct agccagacgt cacaaatcgc cgcgacgcca cctaatagcc ccatcattga
    20461 gccgacggaa aggtcgattt cagcagaaat tatgacgaac accattccta ccgcgaggat
    20521 gccggtaatc gcggtctggc gtaacaggtt ggagacgtta cgggcgctta agtaggcacc
    20581 atcggtggtc caggtaaaga acagcatgat tgcgatgata gctgcaatca tcacgaagac
    20641 ctgcaaattc agtgatttca gcccggagaa gccaccggat gtcggtacgg ccaatttcac
    20701 ttcagacgga ttgcttttcg acatgatgtt cgctcctcaa tgcggcttcc atcacctgct
    20761 cctgagtcag gttatgattt atcaggttgg cttttagttt cccttcatgc attactagta
    20821 cacgatcgct aaggccgagc acttcaggta attcagaaga gatgacaata acggcaatac
    20881 cctgctggac gagttggtta attaatttgt agatctcgta tttcgcgcca atatcgatac
    20941 ccctggtggg ttcatcaaga atgagaatgc gcgggttaag taacagacag cgagcgagga
    21001 tcgctttttg ctgattgccg ccgctcaaac gtccaatagc aaggtcgggg gacgacgttt
    21061 taactttgag ttgctggatt gattccagaa tacatttttg ctctgccgcg tcatcaagct
    21121 ggctaatgcc accggtaaat ttattgagtg cggcgagggt aatattttta ccaaccgcca
    21181 ttaccggaac gatgccgtcg cgctttctgt cttcggggac catcgcaatc ccctgggcga
    21241 tggcttgctg acagttacga atatctacct gtttgccatc aatataaatt tttccttccc
    21301 attgtccggg ccacacacca aacaggcact gaatggtctc ggtacgtccg gcaccaacga
    21361 gtccggcaat acccagtatt tcgccacgtt tcagggaaaa cgagacatca ttaactcgtt
    21421 taatatgacg attaaccgga tgccatgccg tcagatgttc aatacgtaat atttcatctc
    21481 cggtggtatg tggttcatta gggtaaagcg cggttaactc tcgcccgacc atcatggtga
    21541 taatatcgtc ttcactcatt ccggcagcat cacgcgtacc aatgtgctgt ccgtcgcgaa
    21601 taacgcaaat cgtatcggaa atcgctttga cttcgttgag tttgtgcgaa atataaatac
    21661 aggcgatacc gtgctgttgt agatcgcgaa taatatccag taaaaccgac gtttcctgct
    21721 cagttaatga ggctgtcggt tcatcgagaa ttaacaagcg cacctgttta ttaagtgcct
    21781 tggcaatttc aaccagttgt tgttgcccaa gccctaaatc gccaacgcgg gtatcaggtg
    21841 aaatggataa actgacctgt gcgagcagct tctgacagcg tagcgtcatc aggtcataat
    21901 ccataatgcc attgtgggtt atttcgttac ccaggaagat attttccagc acggtcaatt
    21961 ctttcaccag ggccaattcc tggtgaatga tggcgatacc tttgcgttcg gtatcgcgga
    22021 tgtgactcgc ctgaatctct tctcccgcaa aaataatttc gccttcgtag gagccatggg
    22081 gataaatacc acacagcact ttcatcagcg ttgatttacc agacccattt tccccacaga
    22141 gtgagacgat ttcgccagca ttcaaccgca agctgacgtt atcaatcgcc ttcacactgc
    22201 cgaaggtttt ggtaatgttc ttcatttcaa gtagataagg catagcgact ccacctaagc
    22261 caattcattc acgcggcatg gagagaaatc acgcccccgc tccgcgctgg ggcgtaacgc
    22321 ttacagctcg ctctctttgt ggaatccgtc tttaattacc gtatctttga tgttgttttt
    22381 attcacatcg atcggtgtca ggaggcggga ggggacatct ttcaggccat tatttagtga
    22441 agtatctgct tttggctcct gaccattgcc caattcaacg gcaatttctg cggcagtatt
    22501 tgccagcaat gtaataggtt tatataccgt catagtttgc gtaccggcag caatacgttt
    22561 aatacctgcg agatccgcat cctggccaga aattgctact ttccctgata aaccttgcgc
    22621 gcttaatgcc tgaattgccc cacctgcggt ggcatcgttt gaggcaacta cagcatcaat
    22681 tttgttatta ttggcggtta gcgcgttttc cataattttc aatgcgtttt ccggtaacca
    22741 gccatcaacc cattggtcac caacgacttt aatttttccg gaatcaacgt aaggttgtaa
    22801 cactttcatt tgtccggcgc ggaacagctt agcgttgtta tctaccggcg agccgcccat
    22861 caggaagtaa ttaccttgcg gaacaatatc gaccagggct tttgcctgca gttcaccgac
    22921 tttttcgtta tcgaaagaaa tataaaaatc gatatccgca tcgttaatca tacggtcgta
    22981 agctaatact ttaatgcctt cttgtttggc ttcttttaca acgttactta atacctgacc
    23041 gttatacgga ataatgacaa gaacatcgac accccggttt atcatgtttt caatctgcga
    23101 catttgtgtt tcttcattgc catttgcaga ctgtacaaat actttcgcgc cgagagattc
    23161 tgcctttttc acaaagatat ctcgatcttt ttgccagcgt tcaagacgga gatcatcaat
    23221 cgccatacct attttgactt ctttggcgtg tgcagcaacg ttggtaagca ggagtgaggt
    23281 gcaaagggtg agtagaatgt tctttatttt catggtgtag ggccttctgt agttagagga
    23341 cagctttaat aagtaacaat caccgcgata aacgtaacca atttttagca actaaacagg
    23401 ggaaaacaat tacagatttt tatctttcga ttacgatttt tggtttattt cttgatttat
    23461 gaccgagatc ttacttttgt tgcgcaattg tacttattgc atttttctct tcgaggaatt
    23521 acccagtttc atcattccat tttattttgc gagcgagcgc acacttgtga attatctcaa
    23581 tagcagtgtg aaataacata attgagcaac tgaaagggag tgcccaatat tacgacatca
    23641 tccatcaccc gcggcattac ctgattatgg agttcaatat gcaagcctat tttgaccagc
    23701 tcgatcgcgt tcgttatgaa ggctcaaaat cctcaaaccc gttagcattc cgtcactaca
    23761 atcccgacga actggtgttg ggcaagcgta tggaagagca cttgcgattt gccgcctgct
    23821 actggcacac cttctgctgg aacggggcgg atatgtttgg tgtgggggcg tttaatcgtc
    23881 cgtggcagca gcctggtgag gcactggcgt tggcgaagcg taaagcagat gtcgcatttg
    23941 agtttttcca caagttacat gtgccatttt attgcttcca cgatgtggat gtttctcctg
    24001 agggcgcgtc gttaaaagag tacatcaata attttgcgca aatggttgat gttctggcag
    24061 gcaagcaaga agagagcggc gtgaagctgc tgtggggaac cgctaactgc tttacaaacc
    24121 ctcgctatgg cgcgggtgcg gcgacgaacc cagatccaga agtcttcagc tgggcggcaa
    24181 cgcaagttgt tacagcgatg gaagcaaccc ataaattggg tggtgaaaac tatgtcctgt
    24241 ggggcggtcg tgaaggttac gaaacgctgt taaataccga tttgcgtcag gagcgtgaac
    24301 aactgggccg ctttatgcaa atggtggttg agcataaaca taaaatcggc ttccagggca
    24361 cgttgcttat cgaaccgaaa ccgcaagaac cgactaaaca tcaatatgat tacgatgctg
    24421 cgacagtcta tggcttcctg aaacagtttg gtctggaaaa agagattaaa ctgaacattg
    24481 aagctaacca cgcgacgctg gcaggtcact ctttccatca tgaaatagcc accgccattg
    24541 cgcttggcct gttcggttct gtcgacgcca accgtggcga tgcgcaactg ggctgggaca
    24601 ccgaccagtt cccgaacagt gtggaagaga atgcgctggt gatgtatgaa attctcaaag
    24661 caggcggttt caccaccggt ggtctgaact tcgatgccaa agtacgtcgt caaagtactg
    24721 ataaatatga tctgttttac ggtcatatcg gcgcgatgga tacgatggca ctggcgctga
    24781 aaattgcagc gtgcatgatt gaagatggcg agctggataa acgcatcgcg cagcgttatt
    24841 ccggctggaa tagcgaattg ggccagcaaa tcctgaaagg ccaaatgtca ctggcagatt
    24901 tagccaaata tgctcaggaa cataatttgt ctccggtgca tcagagtggt cgccaggagc
    24961 aactggaaaa tctggtaaat cattatctgt tcgacaaata acggctaact gtgcagtccg
    25021 ttggcccggt tatcggtagc gataccgggc atttttttaa ggaacgatcg atatgtatat
    25081 cgggatagat cttggcacct cgggcgtaaa agttattttg ctcaacgagc agggtgaggt
    25141 ggttgcttcg caaacggaaa agctgaccgt ttcgcgcccg catccactct ggtcggaaca
    25201 agacccggaa cagtggtggc aggcaactga tcgcgcaatg aaagctctgg gcgatcagca
    25261 ttctctgcag gacgttaaag cattgggtat tgccggccag atgcacggag caaccttact
    25321 ggatgctcaa caacgggtat tgcgccctgc cattttgtgg aacgacgggc gctgtgcgca
    25381 agagtgcgct ttgctggaag cgcgagttcc gctatcgcgg gtgattaccg gcaacctgat
    25441 gatgcccgga tttactgcgc ctaaattgct atgggttcag cggcatgagc cggagatatt
    25501 ccgtcaaatc gacaaagtat tattaccgaa agattacttg cgtctgcgta tgacggggga
    25561 gtttgccagc gatatgtctg acgcagctgg caccatgtgg ctggatgtcg caaagcgtga
    25621 ctggagtgac gtcatgctgc aggcttgcga cttatctcgt gaccagatgc ccgcattata
    25681 cgaaggcagc gaaattactg gtgctttgtt acctgaagtt gcgaaagcgt ggggtatggc
    25741 gacggtgcca gttgtcgcag gcggtggcga caatgcagct ggtgcagttg gtgtgggaat
    25801 ggttgatgct aatcaggcaa tgttatcgct ggggacgtcg ggggtctatt ttgctgtcag
    25861 cgaagggttc ttaagcaagc cagaaagcgc cgtacacagc ttttgccatg cgctaccgca
    25921 gcgttggcat ttaatgtctg taatgctgag tgcagcgtcg tgtctggatt gggccgcgaa
    25981 attaaccggc ctgagcaatg tcccagcttt aatcgctgca gctcaacagg ctgatgaaag
    26041 tgccgagcca gtttggtttc tgccttatct ttccggcgag cgtacaccac acaataatcc
    26101 ccaggcgaaa ggggttttct ttggtttgac tcatcaacat ggccccaatg aactggcgcg
    26161 agcagtgctg gaaggcgtgg gttatgcgct ggcagatggc atggatgtcg tgcatgcctg
    26221 cggcattaaa ccgcaaagtg ttacgttgat tggtggcggg gcgcgtagtg agtactggcg
    26281 tcagatgctg gcggatatca gcggtcagca gctcgattac cgtacggggg gggatgtggg
    26341 gccagcactg ggcgcagcaa ggctggcgca gattgcggcg aatccagaga aatcgctcat
    26401 tgagttgttg ccgcaactac cgttagaaca gtcgcatcta ccagatgcgc agcgttatgc
    26461 cgcttatcag ccacgacgtg aaacattccg tcgcctctat cagcaacttc tgccattaat
    26521 ggcgtaaacg ttatcccctg cctgaccggg tgggggataa ttcacatcta tatatctcag
    26581 caattaatta atatttagta tgaatttatt ctgaaaatca tttgttaatg gcatttttca
    26641 gttttgtctt tcgttggtta ctcgtaatgt atcgctggta gatatggaga tcgttatgaa
    26701 aacctcaaaa actgtggcaa aactattatt tgttgtcggg gcgctggttt atctggttgg
    26761 gctatggatc tcatgcccat tgttgagtgg aaaaggctat tttcttggcg tgttaatgac
    26821 agcaactttt ggcaactatg catatcttcg cgcagaaaaa ctcgggcaac tggataattt
    26881 ttttacccat atttgccagt tagttgcgtt aatcactatc ggtctcttgt ttatcggtgt
    26941 tttaaatgca cctatcaatg cttatgaaat ggtgatctac cccatcgcct tttttgtctg
    27001 cttgtttggt caaatgcgtt tgtttcgctc ggtatgagca acataaagct cttacacatt
    27061 caggaatgaa aggaatactg tgatggacaa caaaatatca acctattcac cggcctttag
    27121 tattgtgtca tggatagctc tcgttggtgg tatcgttacc tatctgttag ggctatggaa
    27181 tgcagagatg cagttaaatg aaaaaggata ttattttgcc gtactggtat taggactgtt
    27241 ttctgcggcg tcttatcaaa agaccgttcg ggacaagtat gaaggcatac cgaccacttc
    27301 catttattat atgacctgcc tgactgtctt tattatctct gttgcattac tgatggtagg
    27361 tctgtggaat gcgacattat tactcagcga aaaaggtttt tatggactgg ctttcttctt
    27421 aagcttgttt ggtgcagtag cggtgcagaa aaatattcgt gatgccggaa taaacccacc
    27481 aaaagaaaca caggttaccc aggaagaata cagcgaataa ctcacgtaag cccggtcagt
    27541 ccaatgtgac cgggctttta cttaactcac taatctgttt ctgtcgattc gttgtaccag
    27601 catagaaagt aacaaactcg ctgccaacgt cgcgcaaaaa atccaaataa tatccagtat
    27661 tggccaattt ttaagctcaa ttccccgggt gcgcagcgca tggataatca aggcgtggaa
    27721 tccgtatata cccaacgaat ggcgggagat taagccaagt ccgcgaatgg tacgcgtatc
    27781 cagcgtgttt ttaaccagag tcaatagcgc gattgcgcag ataaaaacca tcggcccaca
    27841 gtaaggatac caggtatcgg caaaatttcc gcgccactgc aattcatata atgtcccgcg
    27901 agagataata aaaactcccg tcgcaaacag tgcggcgctc acccacgaca gtgctttatg
    27961 ctgtgtgtcc atcatcccta aagcgcggcc caacatgcca tacagaatgt agtaaaaagt
    28021 atcgccattg atatataagt taattggcag ccattcaaaa ccgtcgattt tctgcggcac
    28081 ggtgtttggg ttagcgatga taccaatcac caccattagc accagcaaca tttttccgcc
    28141 gacgttcttc acctgaatca gcggtgaaac cagataaatc accgcaatcg cgaagaaaaa
    28201 ccataagtgg taaaacactg gcttttgcag caggtttttc agcgctaact ccacattaat
    28261 ggaggtaaac agtgcaatgt agagcagcgc gattgcgcta taaaaaaaca gacataagcc
    28321 gatacgcaag aaatggcgcg gctgggcgct gcgttcgcca aaaaagagat agccggaaat
    28381 catgaaaaat agcggcacgc tgacacgaga ggcagagttc agaacattgg cgatatccca
    28441 ggtgacgggg ctaacactat gagcattggt cacataccag gtagtggtgt gaatcatcac
    28501 caccattaaa cacgctatcc ctcgcaggtt atcaatccag taaattttgg gctgcatctg
    28561 tgtctctgta tctggttaaa aaaagtctga ccgataaatc attcagttgg cgcactggaa
    28621 taatctgagt tttaacacta cagcttatag aggcttaagg aaattcgtaa gatatcagcc
    28681 actataccga tataaataat aagactcacc tgcaaaccag acggtaattt aatgatgatg
    28741 aactctttct ttccggcaat ggcgcttatg gtgctagtgg gttgttctac accgtcaccc
    28801 gtgcagaaag cacaacgggt aaaggttgat cctctgcgtt cgttgaatat ggaagcgtta
    28861 tgcaaggatc aggcggcaaa acgttataac accggcgagc aaaaaatcga cgtcaccgcc
    28921 ttcgaacagt tccagggaag ctatgaaatg cgcggttata ccttccgtaa agagcagttt
    28981 gtctgttctt ttgacgcaga tggccatttt ttgcatcttt ccatgcgtta agccctgctt
    29041 tttcccgttt catactgtat atcttccatc cagcgggtat actgatccct tcctttaaat
    29101 ccacacgtat ccagcacgaa ataatatgca aaagtttgat accaggacct tccagggctt
    29161 gatcctgacc ttacaggatt actgggctcg ccagggctgc accattgttc aaccattgga
    29221 catggaagtc ggcgcgggaa cctctcaccc aatgacctgt ctgcgcgcgc tggggccaga
    29281 accgatggcg gctgcttatg ttcagccttc tcgtcgcccg accgatggtc gctacggcga
    29341 aaaccccaac cgtttacagc actactatca gttccaggtg gtcattaagc catcgccgga
    29401 caatattcag gagctgtacc ttggttctct gaaagagctg ggcatggacc cgactatcca
    29461 cgatatccgt ttcgtggaag ataactggga aaacccgacg ctgggtgcct ggggactggg
    29521 ctgggaagtg tggctaaacg gcatggaagt gacgcagttc acttacttcc agcaggttgg
    29581 tggtctggag tgtaaaccgg ttaccggcga gatcacctac ggtctggaac gtctggccat
    29641 gtacattcag ggcgtagaca gcgtttacga cctggtctgg agcgacggcc cgctgggtaa
    29701 aaccacctac ggcgacgtgt tccatcagaa cgaagtggag cagtccactt acaacttcga
    29761 atacgcggat gtggacttcc tgttcacctg ctttgagcag tacgagaaag aagcgcagca
    29821 gctgctggcg ctggaaaatc cgctgccgct gccagcttac gagcgtattc tgaaagccgc
    29881 ccacagcttc aacctgctgg atgcgcgtaa agccatctcc gtcaccgagc gtcagcgcta
    29941 cattctgcgc attcgcaccc tgaccaaagc agtggcagaa gcatattacg cttcccgtga
    30001 agccctaggc ttcccgatgt gcaacaaaga taagtaagag gcggctatgt ctgagaaaac
    30061 ttttctggtg gaaatcggca ctgaagagct gccaccaaaa gcactgcgca gcctggcaga
    30121 gtcctttgct gcgaacttta ctgcggagct ggataacgca ggcctcgcac acggcaccgt
    30181 tcaatggttt gctgctccgc gtcgtctggc gctgaaagta gctaacctgg cggaagcgca
    30241 accggatcgt gaaatcgaaa aacgcggccc ggcgattgcc caggcgttcg acgctgaagg
    30301 caaaccgagc aaagcggcag aaggttgggc gcgtggttgc ggtattaccg ttgaccaggc
    30361 tgagcgtctg actaccgata aaggcgaatg gctgctgtat cgcgcccatg tgaagggcga
    30421 aagcactgaa gcactgctgc cgaatatggt tgcgacttcg ctggcgaaac tgccgattcc
    30481 gaaactgatg cgctggggtg caagcgacgt gcacttcgtg cgtccggtgc acaccgtgac
    30541 cctgctgctg ggcgacaaag tcattcctgc aaccattctg ggcattcagt ccgatcgcgt
    30601 gattcgcggc caccgcttta tgggcgagcc ggaattcacc atcgataacg ccgatcagta
    30661 tccggaaatt ctgcgtgagc gtgggaaagt catcgccgat tacgaagaac gtaaggcgaa
    30721 gattaaagcc gatgccgaag aagcagcgcg taagattggc ggtaacgctg acttaagcga
    30781 aagcctgctg gaagaagtgg cttcgctggt ggagtggccg gtcgttctaa ccgcgaaatt
    30841 tgaagagaaa ttcctcgcgg tgccgtctga agcactggtt tacaccatga aaggtgacca
    30901 gaagtacttc ccggtgtatg cgaacgacgg caaactgctg ccgaacttta tcttcgttgc
    30961 caacatcgaa tcgaaagatc cgcagcaaat tatctctggt aacgagaaag tcgttcgtcc
    31021 acgtctggct gatgccgagt tcttcttcaa caccgaccgt aaaaaacgtc tggaagataa
    31081 cctgccgcgc ctgcaaaccg tgctgttcca gcaacagctg ggtacgctgc gtgacaagac
    31141 tgaccgcatc caggcgctgg ctggctggat cgccgaacag attggcgctg acgttaacca
    31201 cgcaacccgt gcgggcctgc tgtctaagtg tgacctgatg accaacatgg tcttcgagtt
    31261 caccgacacc caaggcgtta tggggatgca ctacgcgcgt cacgatggcg aagcggaaga
    31321 cgtagccgtc gcgctgaatg agcagtatca gccgcgcttt gccggtgatg acctgccgtc
    31381 caacccggta gcttgtgcgc tggcgattgc tgataagatg gatactctgg cgggtatctt
    31441 cggtatcggt cagcatccga aaggcgacaa agacccgttt gcgctgcgtc gtgccgcact
    31501 tggcgtgctg cgaattatcg ttgagaagaa cctcaacctt gatctgcaaa cgctgaccga
    31561 agaagcggtg cgtctgtatg gcgataagct gactaatgcc aacgtagttg atgatgttat
    31621 cgactttatg ctcggtcgct tccgcgcctg gtatcaggac gaaggttata ccgttgacac
    31681 catccaggcg gtactggcgc gtcgtccgac tcgtccggct gatttcgatg cccgcatgaa
    31741 agcggtatcg cacttccgta ccctggaagc agctgcagca ctggcggcgg cgaacaaacg
    31801 tgtatctaac atcctggcga aatctgacga agtgctgagc gatcgcgtga atgcctctac
    31861 tctgaaagag ccggaagaaa ttaaactggc gatgcaggtt gtggtgctac gtgacaagct
    31921 ggagccgtac tttgcggaag gtcgttacca ggatgcgctg gtcgaactgg cagagctgcg
    31981 tgaaccagtc gatgccttct tcgataaagt gatggtcatg gttgatgaca aagaattgcg
    32041 tatcaaccgt ctgaccatgc tggagaaact gcgcgaactg ttcctgcgcg ttgcggatat
    32101 ttcgctgttg cagtaataac gccgttatta aaaagcctgc catctggcag gcttttttta
    32161 tcgctaaata atacagcaac ctttaataat cttctgctga ataaagatta tctcatatat
    32221 taattttatg agattttttt aggattatat caaggagaag aaacaaactt attaagctag
    32281 aatagccacg ggtgcttgag actgtttgtc tcaggtattc accgaaaggc agacagagaa
    32341 aagccccacc tgactataaa tcaaagtgag gcccctctat gtatagacac cataaggtta
    32401 gcctcttact cgttggaaat caacacaggg ggctgggaat gccgcagaaa tatagattgc
    32461 tttctttaat agtgatttgt ttcacgcttt tatttttcac ctggatgata agagattcac
    32521 tgtgtgaatt gcatattaaa caggggagtt atgagctggc ggcattttta gcctgcaatt
    32581 tgaaagagta agagtcttcg gcggaaagtt attcccgcct ttcttacggc gttgcgcatt
    32641 ctcattgcac ccaaatttat ttttcacaaa aataatacta gattttatta cgcgatcgat
    32701 tatttatttc ctgaaaaaca aataaaaaaa tccccgccaa atggcaggga tcttagattc
    32761 tgtgctttta agcagagatt acaggctggt tacgttgcca gctgccgggc ctttagcgcc
    32821 gctttcgatg gtgaaggaca ctttctgacc ttcgtccaga gatttgtaac catcgttctg
    32881 gatagcagag aagtgtacga acacgtcttt agagccatcg tcaggagtga tgaagccgaa
    32941 gcctttgtca gcgttgaacc attttacgat accagtcatt ttaccggaca tagtgtatta
    33001 cctttaataa ttaagtgtgc ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt
    33061 aaaggaatgt gcactacgag gggtatcaac gataactctt gaagggactt gccttactac
    33121 actgctttaa tggtctgtac gtcaaaccgt tgatgtgcat taagccacgc attggcgggt
    33181 gatgcaacaa ttatttttca tatttatgat taatcggttg gaaaacggtc ctgtcatcag
    33241 gaccgtaaac agcaataaag tggataaaag tctattccat caactgctta cttaatgccg
    33301 ggttggcttg aatcaaacgc atcaatttta gttcggcact tgaaggcttc acgcgtctgg
    33361 attcccattc ctttaccatg gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt
    33421 ttaatcctgt cccttttcgt aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca
    33481 gggtaatttt ctgcgtttca tctttaaaaa caatctgttc caggctgctc aacagctcat
    33541 gcattggatc tttatattcc attgaaaact cctcaaatca cactgcggga tcgtgaattg
    33601 catcgaagct cattaagaat agtcgggaaa agcagacaca gcggggtgaa gtcagggcat
    33661 gctctggcta tgattcgatt cagccagtca tttagcgtga cttatagcca cctaacgatc
    33721 tgataaatgc cgatatatct ctaaccactc ctggtttgta aagatttcga aaatctaacc
    33781 ttctgcgaag gcgtaaatct ctggctacaa aaaaggcaac ggcaatatca ttttttaaca
    33841 atgaaacaac aaattaacct tgtttcattt tctccatgat tttaccagga agattacgta
    33901 gaaagggtat cctgcaagcc tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc
    33961 gttgtgcgct ttttttgggt gaaaggagta agaaaatggc gacaggaaag tcctgctctc
    34021 gctggtttgc gcctcttgcg gcgttattaa tggtagttag cctgagtggg tgttttgata
    34081 aagaaggcga tcagcgtaaa gcgtttatcg acttcctgca gaatacagtg atgcgtagcg
    34141 gtgaacgtct accaaccctg actgccgatc agaaaaaaca gtttggtcct tttgtctctg
    34201 attacgcgat tctgtatggt tattctcagc aggtgaatca ggcgatggat tccggtctgc
    34261 gcccggttgt agacagcgtt aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg
    34321 gtccgctgcg tgaaatgaat ggttcgctgg gtgtgctggc gcaacaactg caaaatgcga
    34381 agttgcaggc cgatgctgcg cactctgcgc taaaacagag tgatgacctg aaaccggtct
    34441 ttgatcaggc attcaccaaa gtggtaacga cgccagctga tgcattgcaa ccgttgattc
    34501 cggcggcaca aactttcacg caacaactgg tgatggttgg agactacatt gcccaacaag
    34561 gtactcaggt gagctttgta gcaaatggca tccagttccc gacttcacag caggcaagtg
    34621 aatataacaa actgattgcg ccattaccgg cacaacatca ggcgtttaat caggcctgga
    34681 ctacagcagt tactgcaacc caataaagag taaaagcccg agcgattatc gggctttact
    34741 ccacgcagtc gcggcttagt ccgcgacgtg cggattcaca cagttcttct caacctttcc
    34801 ttgtaacgca tcaatcaaat tatccacggc acaggcggcc atgccataac gcgtctcatg
    34861 ggtggcagat ccaatatgcg gtactgcgac gacgttggcc attgagagca acggcgaatc
    34921 tacggaaagt ggctcttgtt cgaagacatc cagcccggcg gcgtgaattt cccctttctg
    34981 caatgctgcg atcagtgcat tttcgtcaac caccgggcca cgtccggcat taatgaaaat
    35041 ggcggaggat ttcattttgg cgaattgttc tgcgccaaac agatgatgcg tctcatcagt
    35101 taacggcagg atcaggcaaa cgaaatctga ctcttgtaac agtgtatcca aatcgcagta
    35161 gcgggcgttg aagcgttctt ctgcttcttt atggtggcgg cgcgcgttat agaggatggg
    35221 catgttgaag ccaaagtgcg cacgttgtgc cagcgctata ccgatccgtc ccatcccgac
    35281 aatgcccagt gttttatggt gaacgtcagt gccgtaccag tccgggccta tgctcgcggt
    35341 ccattcgcct gcttttaccc gttctgctac ctccacaacc cgacgagcgg tagacaacac
    35401 cagcgccatc agcgtatcgg cgacggtttc tgttaagacg gttggcgtgt gcatcagcag
    35461 aatttttcgg gcggtaagcg catcgacatc aaaattgtca tagccgacgg agatcgttga
    35521 tgtggcacgc agtttcggca ttttttccag caatgcggca tcaacattct cgtttgaacc
    35581 cagtaaacct tcagcttcgg caaaaattgc tgcattttgt tcgacggttt gtgggctgag
    35641 gtttgccacc tggtgaacgg tgaaatgctc ttgcaggcgt tgcagtaaat catcaggtaa
    35701 ggctttgtag aggataacgg acggcttcat gcttctctcc attcactgat aaggcgatca
    35761 ccttagcctg aaatatgatg aaggaaaact gaagaatgcc aggtgatttc tgcatcacct
    35821 ggcatgaaag ggattacagc gggcttaagg taatttctac acggcggttt tgcgccttac
    35881 cttctgcggt gctgttgctg gcgattgggt tagccgggcc aaggccctga gtacggatgc
    35941 ggctggcgtc cacgccctgg gtgatcaacg cgctggcaac ggaatccgca cgctgctggg
    36001 agagacgcat gttcagatcg tgaccacccg tgctgtcagt ataaccaatc acgttaaccg
    36061 ccgttttcgg atactctttc agcaccattg ccacgccggt cagggtgtta gcgcccgccg
    36121 gtttcagggt tgcgctgctg ctgtcgaagg tcacattgtt cggcatattg aggataatgt
    36181 tatccccgct gcgggttacg ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt
    36241 cctgcacatc catgtaataa cccacgccgc cgcccagagc tgcgcctgct gctgcgccaa
    36301 tcagcgcgcc tttaccgcga tctttcttcg aagaagagag cgcaccaata cccgcgccca
    36361 cgagagagcc cagacctgcg ccgatagcag atttacctgc ttcgcgttcg ccggtgtaag
    36421 ggttagttgt gcagccagat accgccagag cgccactcac tacggcggca ataagataaa
    36481 cacgtttctt cattgttaat ccttaataac ctttttattc tttgccacgg gttccgtggc
    36541 gggagattat gccgcgtgaa catgaagatt attcctggga atactcggaa atttgtaagt
    36601 aatatttaac tgctcaatac atctaacctt tcaggagtct tcggtttgac caactcatcc
    36661 tcacgatatt ccgttctgac tgccgcccat tgggggccca tgctggttga aaccgacggc
    36721 gaaaccgtgt ttagctcgcg tggcgcgtta gccacaggaa tggaaaactc cttgcagagc
    36781 gcggttcgtg accaggttca cagcaatacg cgggtacgat ttccaatggt gcgcaaaggc
    36841 tttctcgcgt caccggaaaa cccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg
    36901 agttgggatg aggcgctgga tcttattcac caacaacata aacgcattcg tgaggcttat
    36961 ggtccggcat cgatttttgc tggttcctac ggctggcgtt caaacggcgt gctgcataag
    37021 gcctcgacat tattacaacg ctatatggcg ctggcaggcg gttataccgg gcatctgggg
    37081 gattattcga ccggcgcggc acaggcgatc atgccgtatg tcgtgggtgg tagtgaagtt
    37141 tatcaacagc agaccagttg gccgctggtg ctggaacata gcgatgtcgt ggtgctgtgg
    37201 agtgctaacc cactcaatac gctgaaaatt gcgtggaatg catccgatga gcaggggctt
    37261 tcttactttt ctgcactgcg tgacagcggg aaaaagctga tctgcattga tccaatgcga
    37321 tcggaaaccg tcgatttctt tggcgataaa atggagtggg tggcaccgca catgggcact
    37381 gatgttgcgc tgatgctggg gatcgcccat acgctggtgg aaaatggttg gcacgacgaa
    37441 gcgtttctgg cgcgttgcac caccggttat gccgtcttcg cctcttattt gctgggcgag
    37501 agtgacggaa tagcgaaaac cgccgaatgg gcagcagaga tttgtggtgt taacgcagag
    37561 aaaatccgcg agctggcggc tattttccac caaaatacca ccatgctgat ggctggctgg
    37621 ggaatgcagc gccaacagtt tggtgagcaa aaacactgga tgatcgtcac gctggcagca
    37681 atgttggggc aaatcggcac acccggcggc ggttttggtc tttcttacca ttttgccaat
    37741 ggtggtaacc ccacgcggcg cgctgcggta ctctcttcca tgcagggcag cttgccgggt
    37801 ggctgcgatg cggtagataa aatccctgtc gcccgcattg ttgaagcact ggaaaacccc
    37861 ggtggcgcat atcaacacaa cggtatggac cgacatttcc cggatattcg ttttatctgg
    37921 tgggcgggcg gtgccaactt tactcatcat caggatacca atcgcctgat ccgtgcctgg
    37981 caaaaaccgg agctggtggt gatctctgaa tgcttctgga cggcggcggc aaaacacgcg
    38041 gatatcgttc tgcctgcgac tacctctttt gagcgtaatg atctcaccat gaccggtgat
    38101 tacagtaatc agcatctggt gccgatgaag caagtggtgc cgccacgcta tgaagcgcgt
    38161 aatgattttg atgtttttgc cgagttaagt gaacgctggg agaagggcgg ttatgcacgt
    38221 tttacggaag gaaaaagtga gctgcaatgg ctggaaacgt tttataacgt tgcccggcag
    38281 cgcggggcaa gccagcaggt tgaattgccg ccatttactg agttctggca agccaaccag
    38341 ttaattgaga tgccggaaaa cccggacagc gagcggttta ttcgcttcgc tgatttttgc
    38401 cgcgatccgc aggcgcatcc attaaaaacc gccagcggta agattgaaat cttctcgcag
    38461 cgtattgccg attacgctta tccggattgc ccggggcatc caatgtggct ggtgccggac
    38521 gaatggcagg gcaatgccga accggaacag ttacaggtac tttctgctca tccggcgcat
    38581 cgtctgcaca gccagctgaa ttacagttct ctgcgcgaat tgtacgcggt ggcaaaccgt
    38641 gagcctgtca ccattcatcc tgacgatgcc caggcgcgcg gcatacaaga tggcgatact
    38701 gttcggctgt ggaacgcacg cgggcaaatt cttgccggag cggtcattag cgagggaatt
    38761 aaacctggcg tgatttgcat tcacgaaggg gcatggccgg atctggattt aaccgctgac
    38821 ggtatttgta aaaacggcgc agtgaacgtg ctgaccaaag atctccccag ctcgcggctg
    38881 gggaatggct gtgcgggtaa tacggcgctg gcatggctag aaaaatacaa cggtccggaa
    38941 ctgacactta cagcgtttga accaccggcc agctcataat ccaggtgggt agttgggttt
    39001 catcctgcca tgcgcaatcg acaatgtgaa aaccctgcgc ctggtaaaaa tctatcgccg
    39061 gttgattttt ttgataaacc tccagcatca gatggggata gcgctgctgc acatactgca
    39121 tcagcgcctt accaataccg cgtctgacgg ccttcggtgc gacaaacatt gctgccagaa
    39181 atcggccttc cataatgctg acaaaaccga gaagcttacc gtcttcttcc cagacccagt
    39241 ttttcgcatt ggcaagatac gcatcccgca ccagcggaat gcactcgcgc cagtaattca
    39301 cttttataaa gggatgcccc caggttgtac tttccagcca cagttcgagg atcgcgggga
    39361 gttctgaacg ttgcgcttcc cgaatcatgg tttatttccc ggatagcaac agcagccaac
    39421 cacatgatca ttcaccagcc cacatgcctg cataaaggag taacagattg tggtaccgac
    39481 aaacttaaaa ccacgttttt tcagtgcctt agatagggcg tcggaggcgg gcgtagatgt
    39541 ggggatttcg ctcaacgttg tggcttgtgt cacctgtggc tgatgattta caaacgacca
    39601 gacaaagtcg gcaaacggtt cgccgttctg ttccatttgc aggtacgccc gcgcattacc
    39661 aataattgcc tgaattttcc cgcgatggcg gataatcccg gcgtcctgta ccagtctttc
    39721 gacatcctct tcctgcattg cggcaacctt caccggatcg aactgatgaa agcaggcgcg
    39781 atagttttcg cgttttttga ggacggtgat ccacgataac ccagcctgct gcccttccag
    39841 gcagatcatt tcgaacagtt ttttcctgtc agtttcaggc acgccccact cattatcatg
    39901 gtaggcaata taaagcgggt cctgactcac ccagccgcaa cgttccatac tttccctcgc
    39961 tatgtacccg atcgtaaaaa tttcagccgc ttttcttgac gcatcggtga aaaagtcaat
    40021 atttaataca gggctactat gcccgatatc cttcatacaa tgacaataat attgccgaat
    40081 tcggctcttc cctggagtcg tgttaatgat cataaaaaaa agcggtggtc gctggcagct
    40141 aagcctgctg gcgagtgtgg taatcagtgc cttttttctc aacacagctt acgcctggca
    40201 acaagaatat atcgttgata cgcaacccgg gcattccaca gagcgttaca cctgggatag
    40261 tgatcatcaa cctgattaca acgatatttt gtcgcaacgt attcaaagta gccaaagggc
    40321 gctgggactg gaagtcaatc tggcggaaga aacccctgtg gatgtgacca gcagtatgag
    40381 tatgggctgg aattttcctt tatatgaaca ggttacaact ggcccggtcg cggcattaca
    40441 ttacgatggc acaaccacct cgatgtataa cgagtttggc gacagtacta ccacgctgac
    40501 cgatccgtta tggcatgcca gcgtgagtac cttaggctgg cgtgttgact cccggcttgg
    40561 cgatctccga ccctgggcgc aaatcagcta taaccagcaa tttggcgaga atatctggaa
    40621 ggcgcaatca ggcctgagcc ggatgacggc gacaaaccag aacggcaact ggctggatgt
    40681 caccgtaggc gctgatatgt tgctcaatca aaatattgcc gcctatgccg cgctatctca
    40741 ggcagaaaat accactaaca atagcgatta tctgtatacg atgggggtta gtgccagatt
    40801 ttaacgtaac aatcacaatt gaaaccattg aataacaata gttgtggcga tagtgggtgc
    40861 taacttacca aataataaat ttggtgaata attgtcgcgt cattcattcc tgaactaagg
    40921 catttcattc cgttctgatg gcatttcatg ccgtttttcc ccaggcataa agtgcacttc
    40981 gttatggttg tcggcagaga tttttccttt ttattactgc aggaatactg ccatgacacc
    41041 ttcaaattat cagcgtaccc gctggttgac actcatcggt actatcatta cccagtttgc
    41101 gctggggtcg gtttatacct ggagcctgtt taacggcgcg ctttccgcca agctggatgc
    41161 gccggtaagc caggtcgctt tctctttcgg cttgttaagt ctggggctgg caatatcgtc
    41221 ttctgttgcg ggcaaattgc aggaacgttt tggtgttaaa cgcgtcacca tcgcttccgg
    41281 cattttgctg ggattagggt tcttcctgac agcgcattct gacaacctga tgatgctgtg
    41341 gttaagcgcc ggtgtgctgg tcggtctggc ggatggcgcg ggttacctgc tgacgctctc
    41401 taactgcgtg aaatggttcc cggagcgtaa aggtctgatc tccgcgttcg ctatcggttc
    41461 ttatggtctg ggtagcctgg gtttcaaatt tatcgacacg cagctgctgg aaacggtcgg
    41521 tctggaaaaa acctttgtga tttggggagc gattgcgctg gtgatgattg ttttcggcgc
    41581 aacgttaatg aaagacgcac caaaacagga agtgaaaacc agcaatggtg tggtggagaa
    41641 agattacacg ctggcagagt cgatgcgtaa accgcagtac tggatgttag cggtaatgtt
    41701 cctgactgcc tgcatgagcg gcctgtacgt gattggggta gcgaaagata tcgcccaaag
    41761 tctggcacac cttgatgtgg tttctgcagc caatgcagtc actgttattt ccatcgccaa
    41821 cctttcaggt cgtctggtgc tgggcattct gtctgacaaa atcgcccgta tccgtgttat
    41881 caccattggt caggtaattt cgctggtggg tatggcggcc ctgctgtttg caccattgaa
    41941 tgcagtgacg ttctttgcag cgattgcctg cgtggcattt aactttggcg gcactatgac
    42001 cgtctttccg tcactggtca gtgagttctt cggcctcaat aacctggcga aaaactacgg
    42061 tgtgatttac ctcggtttcg gtatcggtag catttgtggt tcgattatcg cctcactgtt
    42121 tggcggcttc tatgtgaagt tctacgtcat ctttgctctg ctgattctgt cattggcgct
    42181 ttctacgacg attcgtcagc cagagcagaa agtattgcgt gaggcgcata tttagccaga
    42241 aactacaggt tttgaacgaa agggggagga aatcctcctt ttttattgca ttttaaagaa
//
LOCUS       DAZFDC010000022        42154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFDC010000022 DAZFDC010000000
VERSION     DAZFDC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.022"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            307..393
                     /gene="leuL"
                     /locus_tag="ACHJ48_001374"
     CDS             307..393
                     /gene="leuL"
                     /locus_tag="ACHJ48_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO4706964.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            486..2057
                     /gene="leuA"
                     /locus_tag="ACHJ48_001375"
     CDS             486..2057
                     /gene="leuA"
                     /locus_tag="ACHJ48_001375"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO4706965.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRATEVEKE
                     LQRKAQHNENNKETV"
     gene            2057..3148
                     /gene="leuB"
                     /locus_tag="ACHJ48_001376"
     CDS             2057..3148
                     /gene="leuB"
                     /locus_tag="ACHJ48_001376"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO4706966.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3151..4551
                     /gene="leuC"
                     /locus_tag="ACHJ48_001377"
     CDS             3151..4551
                     /gene="leuC"
                     /locus_tag="ACHJ48_001377"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO4706967.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGILL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4562..5167
                     /gene="leuD"
                     /locus_tag="ACHJ48_001378"
     CDS             4562..5167
                     /gene="leuD"
                     /locus_tag="ACHJ48_001378"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO4706968.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFSQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5275..5406)
                     /gene="sgrT"
                     /locus_tag="ACHJ48_001379"
     CDS             complement(5275..5406)
                     /gene="sgrT"
                     /locus_tag="ACHJ48_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO4706969.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5495..7153
                     /gene="sgrR"
                     /locus_tag="ACHJ48_001380"
     CDS             5495..7153
                     /gene="sgrR"
                     /locus_tag="ACHJ48_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO4706970.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     ITSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQVDAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7382..7951)
                     /gene="espY2"
                     /locus_tag="ACHJ48_001381"
     CDS             complement(7382..7951)
                     /gene="espY2"
                     /locus_tag="ACHJ48_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFO4706971.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFQSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDKLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8193..8292
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8323..9306
                     /gene="thiB"
                     /locus_tag="ACHJ48_001382"
     CDS             8323..9306
                     /gene="thiB"
                     /locus_tag="ACHJ48_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO4706972.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9282..10892
                     /gene="thiP"
                     /locus_tag="ACHJ48_001383"
     CDS             9282..10892
                     /gene="thiP"
                     /locus_tag="ACHJ48_001383"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO4706973.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFSVIEKIPGRNVKTD"
     gene            10876..11574
                     /gene="thiQ"
                     /locus_tag="ACHJ48_001384"
     CDS             10876..11574
                     /gene="thiQ"
                     /locus_tag="ACHJ48_001384"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO4706974.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGALTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(11661..12425)
                     /gene="yabI"
                     /locus_tag="ACHJ48_001385"
     CDS             complement(11661..12425)
                     /gene="yabI"
                     /locus_tag="ACHJ48_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4706975.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12511..13389)
                     /gene="araC"
                     /locus_tag="ACHJ48_001386"
     CDS             complement(12511..13389)
                     /gene="araC"
                     /locus_tag="ACHJ48_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO4706976.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13728..15428
                     /gene="araB"
                     /locus_tag="ACHJ48_001387"
     CDS             13728..15428
                     /gene="araB"
                     /locus_tag="ACHJ48_001387"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO4706977.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQIDASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15439..16941
                     /gene="araA"
                     /locus_tag="ACHJ48_001388"
     CDS             15439..16941
                     /gene="araA"
                     /locus_tag="ACHJ48_001388"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO4706978.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17141..17836
                     /gene="araD"
                     /locus_tag="ACHJ48_001389"
     CDS             17141..17836
                     /gene="araD"
                     /locus_tag="ACHJ48_001389"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO4706979.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17911..20262
                     /gene="polB"
                     /locus_tag="ACHJ48_001390"
     CDS             17911..20262
                     /gene="polB"
                     /locus_tag="ACHJ48_001390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO4706980.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLVSSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEATKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYFRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20426..23332
                     /gene="rapA"
                     /locus_tag="ACHJ48_001391"
     CDS             20426..23332
                     /gene="rapA"
                     /locus_tag="ACHJ48_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO4706981.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23344..24003
                     /gene="rluA"
                     /locus_tag="ACHJ48_001392"
     CDS             23344..24003
                     /gene="rluA"
                     /locus_tag="ACHJ48_001392"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO4706982.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFRERVPKKQYVTRVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            24216..24394
                     /locus_tag="ACHJ48_001393"
                     /pseudo
     CDS             24216..24394
                     /locus_tag="ACHJ48_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(24421..25143)
                     /gene="espY1"
                     /locus_tag="ACHJ48_001394"
     CDS             complement(24421..25143)
                     /gene="espY1"
                     /locus_tag="ACHJ48_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFO4706983.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDMKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGNMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLREAYL
                     PGVDMRDCYLGLKTMKGHNKVLYLEPGWNANLDGATLDGATHLYDEVIIINKITPKKI
                     DTEEVATKQSTAEQITDNAIIE"
     gene            complement(25438..26253)
                     /gene="djlA"
                     /locus_tag="ACHJ48_001395"
     CDS             complement(25438..26253)
                     /gene="djlA"
                     /locus_tag="ACHJ48_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO4706984.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26508..28862
                     /gene="lptD"
                     /locus_tag="ACHJ48_001396"
     CDS             26508..28862
                     /gene="lptD"
                     /locus_tag="ACHJ48_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO4706985.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINANHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVSIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28915..30201
                     /gene="surA"
                     /locus_tag="ACHJ48_001397"
     CDS             28915..30201
                     /gene="surA"
                     /locus_tag="ACHJ48_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO4706986.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30201..31190
                     /gene="pdxA"
                     /locus_tag="ACHJ48_001398"
     CDS             30201..31190
                     /gene="pdxA"
                     /locus_tag="ACHJ48_001398"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO4706987.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            31187..32008
                     /gene="rsmA"
                     /locus_tag="ACHJ48_001399"
     CDS             31187..32008
                     /gene="rsmA"
                     /locus_tag="ACHJ48_001399"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO4706988.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPFQES"
     gene            32011..32388
                     /gene="apaG"
                     /locus_tag="ACHJ48_001400"
     CDS             32011..32388
                     /gene="apaG"
                     /locus_tag="ACHJ48_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO4706989.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32395..33243
                     /gene="apaH"
                     /locus_tag="ACHJ48_001401"
     CDS             32395..33243
                     /gene="apaH"
                     /locus_tag="ACHJ48_001401"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO4706990.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            complement(33240..33554)
                     /locus_tag="ACHJ48_001402"
     CDS             complement(33240..33554)
                     /locus_tag="ACHJ48_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO4706991.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(33557..33790)
                     /locus_tag="ACHJ48_001403"
     CDS             complement(33557..33790)
                     /locus_tag="ACHJ48_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO4706992.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(33876..34355)
                     /gene="folA"
                     /locus_tag="ACHJ48_001404"
     CDS             complement(33876..34355)
                     /gene="folA"
                     /locus_tag="ACHJ48_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO4706993.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34547..36409)
                     /gene="kefC"
                     /locus_tag="ACHJ48_001405"
     CDS             complement(34547..36409)
                     /gene="kefC"
                     /locus_tag="ACHJ48_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO4706994.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYICLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36402..36932)
                     /gene="kefF"
                     /locus_tag="ACHJ48_001406"
     CDS             complement(36402..36932)
                     /gene="kefF"
                     /locus_tag="ACHJ48_001406"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO4706995.1"
                     /translation="MILIIYAHPYPHHSHANKRMFEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(37040..38371)
                     /gene="yaaU"
                     /locus_tag="ACHJ48_001407"
     CDS             complement(37040..38371)
                     /gene="yaaU"
                     /locus_tag="ACHJ48_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4706996.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYILVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38429..38716)
                     /gene="fixX"
                     /locus_tag="ACHJ48_001408"
     CDS             complement(38429..38716)
                     /gene="fixX"
                     /locus_tag="ACHJ48_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO4706997.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38713..39999)
                     /gene="fixC"
                     /locus_tag="ACHJ48_001409"
     CDS             complement(38713..39999)
                     /gene="fixC"
                     /locus_tag="ACHJ48_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4706998.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTNVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(40050..40991)
                     /gene="fixB"
                     /locus_tag="ACHJ48_001410"
     CDS             complement(40050..40991)
                     /gene="fixB"
                     /locus_tag="ACHJ48_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO4706999.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(41006..41776)
                     /gene="fixA"
                     /locus_tag="ACHJ48_001411"
     CDS             complement(41006..41776)
                     /gene="fixA"
                     /locus_tag="ACHJ48_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO4707000.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10137 a        11477 c        10944 g         9596 t
ORIGIN      
        1 tcaaaaaatc cgcaaaacat ataattcaat acaaatcatc aggataggtt ttgcaacgcg
       61 tgcattttgt cccctttttc ctcgtggatt agatgcaaaa atttattctg aaatatgtca
      121 accgatgaaa agcatcggta gttaagcaga aattaatatc gcttgtttta accacagcag
      181 cacaattagc taatcttacg gatgcagaac tcatgctggc gggacgtttt tattgcgtca
      241 gggttgacat ccgtttttgt atccagtaac tctaaaagca tatcgcattc atctggagct
      301 gatttaatga ctcacatcgt tcgctttatc ggtctactac tactaaacgc atcttctttg
      361 cgcggtagac gagtgagcgg catccagcat taagccagca cgcagtcaaa caaaaaaccc
      421 gcgccattgc gcgggttttt ttatgcccga agcgaggcgc tctaaaagag acaaggaccc
      481 aaaccatgag ccagcaagtc attattttcg ataccacatt gcgcgacggt gaacaggcgt
      541 tacaggcaag cttgagtgtg aaagaaaaac tgcaaattgc gctggccctt gagcgtatgg
      601 gtgttgacgt gatggaagtc gggttccccg tctcttcgcc gggtgatttt gaatcagtgc
      661 aaaccatcgc tcgccaggtt aaaaacagcc gtgtatgcgc gttagctcgc tgcgtggaga
      721 aagatatcga cgtggcggcc gaatccctga aagtcgccga agccttccgt attcatacct
      781 ttattgccac ttcgccaatg cacatcgcca ccaagctgcg cagcacgctg gacgaggtga
      841 tcgaacgcgc tatctatatg gtgaaacgcg cccgtaatta caccgatgat gttgaatttt
      901 cttgcgaaga tgccgggcgt acacccattg ccgatctggc gcgagtggtc gaagcggcga
      961 ttaatgccgg tgccaccacc atcaacattc cggacaccgt gggctacacc atgccgtttg
     1021 agttcgccgg aatcattagc ggcctgtatg aacgcgtgcc taacatcgac aaagccatta
     1081 tttccgtaca tacccacgac gatttgggcc tggcggtcgg caactcactg gcggcggtac
     1141 atgccggtgc acgccaggtg gaaggtgcaa tgaacgggat cggcgagcgt gccggtaact
     1201 gttccctgga agaagtcatc atggcgatta aagttcgtaa ggatattctc aacgtccaca
     1261 ccgccattaa tcaccaggag atatggcgca ccagccagtt agttagccag atttgtaata
     1321 tgccgatccc ggcaaacaaa gccattgttg gcagcggcgc attcgcacac tcctccggta
     1381 tccaccagga tggcgtgctg aaaaaccgcg aaaactacga aatcatgaca ccagaatcta
     1441 ttggtctgaa ccaaatccag ctgaatctga cctctcgttc ggggcgtgcg gcggtgaaac
     1501 atcgcatgga tgagatgggg tataaagaaa gtgaatataa tttagacaat ttgtacgacg
     1561 ccttcctcaa gctggcggac aaaaaaggtc aggtgtttga ttacgatctg gaggcgctgg
     1621 ccttcatcgg taagcagcaa gaagagccgg agcatttccg tctggattac ttcagcgtgc
     1681 agtctggctc taacgatatc gccaccgccg ccgtcaaact ggcctgcggc gaagaagtca
     1741 aagcagaagc cgccaacggt aacggtccgg tcgatgccgt ctatcaggcg attaaccgca
     1801 tcactgacta taacgtcgaa ctggtgaaat acagcctgac cgccaaaggt cacggtaaag
     1861 atgcgctggg tcaggtggat attgtcgcca actacaacgg tcgccgcttc cacggcgtcg
     1921 gcctggccac cgatattgtc gagtcctccg ccaaagccat ggtgcacgta cttaacaata
     1981 tctggcgtgc cacagaagtc gaaaaagagt tgcaacgcaa agctcaacac aacgaaaaca
     2041 acaaggaaac cgtgtgatgt cgaagaatta ccatattgcc gtattgccgg gggatggcat
     2101 tggtccggaa gtgatgaccc aggcgctgaa agtgctggat gccgtgcgca accgctttgc
     2161 gatgcgcatc actaccagcc attacgatgt aggcggcgca gccattgata accacgggca
     2221 accactgccg cctgcgacgg ttgaaggttg tgagcaagcc gatgccgtgc tgtttggctc
     2281 ggtaggcggc ccgaaatggg aacatttacc accggaccag caaccagaac gcggcgcgct
     2341 gttgcctttg cgtaagcact tcaaattatt cagcaacctg cgtccggcaa aactgtatca
     2401 ggggctggaa gcattctgtc cgctgcgtgc agacattgcc gcaaacggct tcgacatcct
     2461 gtgcgtgcgc gaactgaccg gcggcatcta tttcggtcag ccaaaaggcc gcgaaggtag
     2521 cggacaatat gaaaaagcgt ttgataccga ggtgtatcac cgttttgaga tcgagcgtat
     2581 tgcccgcatc gcgtttgaat ctgctcgtaa acgtcgccat aaagtgacgt cgatcgataa
     2641 agccaacgtg ctgcaatcct ctattttatg gcgtgaaatc gtcaacgaga tcgccacgga
     2701 atacccggat atcgaactgg cgcatatgta catcgacaac gccaccatgc agctgattaa
     2761 agatccatca cagtttgacg ttctgctgtg ctccaacctg tttggcgaca ttctgtctga
     2821 cgagtgcgca atgatcactg gctcgatggg gatgttgcct tccgccagcc tgaacgagca
     2881 aggttttgga ctgtatgaac cggcgggcgg ctcggcacca gatatcgcag gtaaaaacat
     2941 cgccaacccg attgcgcaaa ttctgtcgct ggcactgctg ctgcgttaca gcctggatgc
     3001 cgatgatgcg gcttccgcca ttgaacgcgc cattaaccgc gcattagaag aaggcattcg
     3061 caccggggat ttagcccgtg gcgctgccgc cgttagtacc gatgaaatgg gcgatatcat
     3121 tgcccgctat gtggcagaag gggtgtaatc atggctaaga cgttatacga aaaattattc
     3181 gacgctcacg tagtgtacga agccgaaaac gaaaccccgc tgttatatat cgaccgccac
     3241 ctggtgcatg aagtgacctc accgcaggcg ttcgatggtc tgcgcgccca cggtcgcccg
     3301 gtacgtcagc cgggcaaaac cttcgccacc atggatcaca acgtctctac ccagactaaa
     3361 gacattaatg cctgcggtga aatggcgcgc atccagatgc aggagctgat caaaaactgc
     3421 aaagaatttg gcgtcgaact gtatgacctg aatcacccct atcaagggat cgtccacgta
     3481 atggggccgg aacagggcgt gaccttgccg gggatgacca ttgtctgcgg cgactcgcat
     3541 accgccaccc acggcgcgtt tggcgcactg gcctttggta tcggcacttc cgaagttgaa
     3601 cacgtactgg caacgcaaac cctgaaacag ggccgtgcga agaccatgaa aattgaagtc
     3661 cagggcaaag ccgcgccggg cattaccgca aaagatatcg tgttggcaat tatcggtaaa
     3721 accggtagcg caggcggcac cgggcatgtg gtggagtttt gcggcgaagc aatccgtgat
     3781 ttaagcatgg aaggtcggat gaccctgtgc aatatggcaa tcgaaatggg cgcgaaagcc
     3841 ggtctggttg cacctgacga aaccaccttt aactatgtca aaggccgtct gcatgcgccg
     3901 aaaggcaaag atttcgacga cgccgttgcc tactggaaaa ccctgcaaac cgacgaaggc
     3961 gcaactttcg ataccgttgt cactttgcag gcagaagaga tttcaccgca ggtcacctgg
     4021 ggaactaacc caggccaggt gatttccgtg aacgacaata ttcccgatcc ggcttcgttt
     4081 gccgatccgg ttgaacgcgc gtcggcagaa aaagcgttgg cctatatggg gctgaaaccg
     4141 ggtattctgc tgaccgaagt ggctatcgac aaagtgttta tcggttcctg caccaactca
     4201 cgtattgaag atttacgcgc ggcggcggaa atcgccaaag ggcggaaagt cgcgccaggc
     4261 gtacaggcgc tggtggttcc cggctctggt ccggtaaaag cgcaggcgga agcagaaggt
     4321 ctggataaaa tctttattga agccggtttt gaatggcgct tgcctggctg ctcaatgtgt
     4381 ctggcgatga acaacgaccg tctgaatccg ggcgaacgtt gtgcctccac cagcaaccgt
     4441 aactttgaag gccgtcaggg gcgcggcggg cgcacgcatc tggtcagccc ggcaatggct
     4501 gccgccgcgg ctgtgaccgg ccatttcgcc gacattcgca acattaaata aggagcacac
     4561 catggcagag aaatttatca aacacacagg cctggtggtt ccgctggatg ccgccaatgt
     4621 cgataccgat gcaatcatcc cgaaacagtt tttgcagaaa gtgacccgta ctggttttgg
     4681 cgcgcatctg tttaacgact ggcgttttct ggatgaaaaa ggccaacagc caaacccgga
     4741 cttcgtgctg aacttctcgc agtatcaggg cgcttccatt ttgctggcac gagaaaactt
     4801 cggctgtggc tcctcgcgtg agcacgcgcc ctgggcattg accgactacg gttttaaggt
     4861 ggtgattgcg ccgagttttg ctgacatctt ctacggcaat agctttaaca accagctgct
     4921 gccggtgaaa ttaagcgacg cagaagtgga tgagctgttt gcgctggtga aagccaatcc
     4981 ggggattcat tttgatgtag acctggaagc gcaagaggtg aaagcgggtg agaaaaccta
     5041 tcgctttacc attgatgcct tccgccgcca ctgcatgatg aacggtctgg acagcattgg
     5101 gcttaccttg cagcacgacg acgccattgc ggcttatgaa gcgaagcaac ccgcgtttat
     5161 gcgttagttt gtacaccgtc cgatactcaa tcgccaaaaa gaaaaaagcc agcagataat
     5221 cactgctggc gtggcgatta acaccaatac tcagtcacac atgatgtccg caaatcagta
     5281 gtcagaatga ctcccctccc actgcatcag ttcttcaagc atggttaatc gttgtgggac
     5341 gcttaaccaa cgcaaccagc acaacttcgc tgtcgcggta aaatagtgct gataaaactg
     5401 acgaatgggg cacctccttg cttcatcgtt aggaattatt gacttaatat agggaaaata
     5461 aaattgctgt cttttgcgca ggagttcccc ttttatgcca tctgctcgtc tgcaacaaca
     5521 gttcatccgc ctgtggcaat gctgcgaggg taaatcgcag gacacaacgc tcaacgaact
     5581 ggcagcgtta ttgagctgct cgcgtcgtca tatgcgtacc ctgctcaaca ccatgcagga
     5641 tcgcggctgg ctgacgtggg aagcggaagt cgggcgtggt aaacgctcgc gtctgacatt
     5701 cctctatacc gggctggcgc ttcagcaaca gcgggcggaa gacctgctgg agcaggaccg
     5761 tatcgaccaa ctagtgcagt tagttggcga caaagcgact gtgaggcaaa tgctggtttc
     5821 tcatctgggc cgcagcttcc gccaggggcg gcacatcctg cgcgtgctct actatcgtcc
     5881 gttgcgtaat ctgctacctg gcagcgcatt gcgccgttcc gaaacccata tcgcccggca
     5941 aatcttcagt tcgctaacgc gcataaatga ggaaaatggg gaactggaag cagacatcgc
     6001 ccaccactgg cagcaaatat caccgcttca ctggcgtttc tttttgcgtc caggagtcca
     6061 ttttcatcat ggtcgtgaac tggaaatgga cgacgtgatc gcctctttaa aacgaatcaa
     6121 tacgctgccg ctctattcgc atattgctga cattgtgtcg ccgacgccct ggacgctgga
     6181 tatccacctc acgcagccgg atcgctggtt gccgttactg ctgggacaag ttccggcgat
     6241 gatcctgccg cgcgaatggg aaaccctcag taactttgcc agccatccca tcggcaccgg
     6301 tccgtatgcg gtgattcgca acagcaccaa tcaactgaaa attcaggcat tcgatgactt
     6361 cttcggttac cgggcattaa tcgatgaagt taacgtctgg gttctgccgg aaattgccga
     6421 cgagccagcc ggagggctga tgctaaaagg tccacagggc gaggaaaaag agattgaaag
     6481 ccgcctggag gaaggttgct actatttact gttcgatagc cgcacccatc gcggggcgaa
     6541 tcagcaagtc agggactggg taagctatgt gctttctcca actaatctgg tctatttcgc
     6601 tgaggaacag taccagcaac tgtggttccc ggcttatgga ctgctccccc gttggcatca
     6661 tgcccgcacc ataacgagcg aaaaaccggc tggtctggaa agcctcaccc tgacctttta
     6721 tcaggatcac agtgagcatc gggtgattgc cgggatcatg cagcagattc tggcaagcca
     6781 ccaggtcaca ctggaaatca aagagatcag ctacgatcag tggcatgaag gagagatcga
     6841 gagcgatatc tggcttaaca gcgccaactt tacgctgccg ctggattttt cgctgttcgc
     6901 gcacctgtgc gaggtaccgc tgctccaaca ctgtattcca atcgactggc aagtcgacgc
     6961 cgcccgctgg cgcaatggcg aaatgaacct ggcgaactgg tgccaacaac tggtcgccag
     7021 caaagcaatg gtgccactta tccaccactg gctgatcatt cagggacaac gcagtatgcg
     7081 cggcctgcgc atgaacaccc tcggctggtt tgattttaaa tcagcgtggt ttgcgccgcc
     7141 ggatccagag tagcttgcgc cttttggcgg tcatgagcgc attttttata ttcagacgga
     7201 atgataaaat acattacttt tatattttct ctgttttatg catcgtctga ttagcgtcgc
     7261 aaataagatt aaagaagggg cattccagcc cctctttcat attctgagtt aatcaataac
     7321 ggcgcagtct agccttgttc tggccggaaa ggtcagcttt ttcaggttaa cctcgcgcag
     7381 gtcagtcata ccaacggcta ttgttcgttt cagcattgct caggtcaacc tcgagacagt
     7441 caataccaca aatgacaacc ccgttaaagt aagcattact cagatcaatc cccccggtcc
     7501 tggtgctctt tttcagctgc aactccagag taaatatcgc ctcatcgcca ataaaaaaac
     7561 tgacgcataa cgtaccttct ggcgagagtg aggccttcgc ggccatattt tccggatctt
     7621 ttgcgtattc tttgagttca ttaaatttta tcagccagtg agttaactta tcacgcgcct
     7681 tcccgtcgac agccccggta aagtattttg agtagtcatc actattctgg aaataaaatg
     7741 catccttcag aatttgcctt gccgcagtct gggaatgttt atctgactgg aagagattgc
     7801 gtaacgtctc ccatagctct tcaaagatat tcatattctc ggtctttttg accaagttag
     7861 ccatcctgat aatctgagaa ttagtaaggc ctggatcttt agtggtatta ctagcaggga
     7921 tactaatctg ttctgggttt cttactttca tgctgatagc tccactctga ataaaaaact
     7981 cagaataagc gtcctgaata agaatactgt caaaaatcgc tcattcacgc aataaacggt
     8041 tacgttacct attttgactg tagcgaaggg attctttcgg atcaacgctc tccagctatg
     8101 caaatacgct cttgatagct gaacgaatat tggctttttt aatacgcctt ctctatggat
     8161 tgctggttga taacaaaatc actacactaa cgccgttctc aacggggtgc cacgcggacg
     8221 cgtgcgctga gaaaataccc gtcgaacctg atccggataa cgccggcgaa gggatttgag
     8281 gctccttctc aagtcctttg ccactctttt ttgaggtgca aagtgttaaa aaaatgtctt
     8341 cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac ccgttctgac tgtttatacc
     8401 tacgattcct tcgccgccga ctgggggcct ggtccggtgg ttaaaaaagc ctttgaagcc
     8461 gactgtaatt gcgaactgaa actggtggcg ctggaagatg gcgtttcgct tctcaaccgt
     8521 ctacggatgg aaggcaaaaa cagtaaagcc gatgtggtgc tggggctgga taacaatctg
     8581 ttagacgccg ccagtaaaac cggactgttt gccaaaagcg gtgtggcagc ggatgccgtt
     8641 aacgttcccg gcggctggaa taatgacatt ttcgtaccgt ttgattatgg ctacttcgcc
     8701 ttcgtttatg acaagaacaa actgaaaaac ccgccacaaa gcctgaaaga actggttgag
     8761 agcgatcaaa actggcgggt gatttatcag gatccgcgca ccagtacacc ggggctgggt
     8821 ctgttgctat ggatgcaaaa agtctatggc gatgacgccc cacaagcctg gcagaaactg
     8881 gcgaagaaaa cggtcacggt caccaaaggc tggagcgaag cctacggcct gtttttaaaa
     8941 ggtgaaagcg atctggtact gagttacacc acctctccgg cttatcacat tctcgaagag
     9001 aagaaagata actacgccgc cgcgaacttc agcgaaggtc actatctgca agtggaagtc
     9061 gccgctcgca ccgctgccag caagcagccg gagctggcgc aaaaattcct ccagtttatg
     9121 gtttctccgg ctttccagaa tgcgatccca accggcaact ggatgtatcc ggtggcaaac
     9181 gtcacgctgc ctgccggttt tgaaaaattg accaaacccg caaccacgct ggagttcacg
     9241 ccagccgaag tggcggcaca acgtcaggca tggattagcg aatggcaacg cgccgtcagc
     9301 cgttaattcc cggctggtta attccaggtg taagcgccgc cacgctggtg gtagcggttg
     9361 cgctggcggc gtttctcgcc ctgtggtgga acgcgccgca gggtgactgg tcggcagtct
     9421 ggcaggacag ctacctgtgg catgtggtgc gcttctcctt ctggcaggcg tttctctcgg
     9481 cgctactctc tgtcgtaccc gcgatattcc tcgcccgcgc gctctatcgc aggcgctttc
     9541 cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt gatcctcccg gtgctggttg
     9601 ctgttttcgg cattcttagc gtctatggtc gccagggctg gctggcatcg ctctgccaat
     9661 cgctcggtct ggagtggacc ttttcgccct acggcctgca aggcattttg ctggcccatg
     9721 tgttttttaa tctgccgatg gcgagccgct tattactcca ggcactggaa aacattcccg
     9781 gcgaacaacg tcagcttgcc gcccagcttg ggatgcgcgg ctggcatttt ttccgcttcg
     9841 tcgaatggcc gtggttacgg cgacaaatcc cgccggttac cgcgcttatc tttatgctct
     9901 gtttcgccag cttcgccacc gtgttatcgc tgggcggcgg tccgcaggca accactatcg
     9961 agctggcaat ctatcaggcg ctgagttacg actacgatcc tgcccgcgcg gcgatgctgg
    10021 cgctgatcca gatggtgtgt tgccttgggc tggtgctgtt gagtcagcga ctgagtaagg
    10081 ccattgcgcc cggcaccacg ctgctgcaag gctggcgcga cccggacgat cgtctgcata
    10141 gccgcatttg cgacacgatg ttaattgtgc tggcgctgct gctgttgctg ccaccgttgc
    10201 tggcggtgat cgtcgatggg gtaaatcgcc agttgccgga agtgctggca caaccggtgc
    10261 tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc ggcaggtgta ttgtgcgtag
    10321 tgctgaccat gatgctgcta tggagcagtc gcgaactgcg ggcgcggcaa aaaatgctgg
    10381 cgggccaggc gctggagatg agcggcatgt tgatcctcgc tatgccgggg attgtgctgg
    10441 caacgggctt ctttttactg ctcaacaaca ctatcggcct gccacaatct gctgacggca
    10501 ttgtgatttt caccaatgcg ttaatggcga tcccctacgc cctgaaagtg ctggaaaacc
    10561 cgatgcgcga tatcaccgcc cgctacagca tgttatgtca gtcgctgggg attgaaggct
    10621 ggtcacgctt aaaagtagtg gagctgcgcg ccctgaaacg cccgctggcg caggcgctgg
    10681 cctttgcctg cgtgctgtcg attggtgatt ttggcgtggt ggcgttgttc ggtaacgatg
    10741 atttccgcac cctgccgttt tatctctacc agcaaattgg ctcctatcgc agccaggacg
    10801 gcgcggtcac cgcgttaatt ctgctgctgc tctgttttct gctgtttagc gtgattgaaa
    10861 aaataccggg gcgaaatgtt aaaactgact gatatcacct ggctttatca ccatttgccg
    10921 atgcgtttta gcttaacggt agaacgcggc gagcaggtgg cgatcctcgg gccaagcggc
    10981 gcgggtaaaa gtaccctgct gaacttgatc gccggttttc tgacaccagc cagcggtgcg
    11041 ctgactatcg acggcgttga tcacaccacg acgccgccgt cacgccgtcc ggtgtcgatg
    11101 ctgtttcagg agaacaacct gttcagccac ctgacggtcg cgcagaacat cgggctgggg
    11161 cttaatccag gattgaagct gaacgcggta cagcaggaga aaatgcacgc gattgcccgt
    11221 cagatgggga ttgataattt aatggcgcgg ttaccgggcg agctttccgg cggtcagcga
    11281 cagcgcgtgg cgttagcgcg ttgtctggta cgcgaacagc cgattttatt gctcgatgaa
    11341 ccgttctctg cgctcgatcc ggcgctacgt caggagatgt tgacgctggt gagcactagc
    11401 tgccagcagc aaaaaatgac gctattgatg gtgtcgcaca gtgtggaaga tgcggcgcgg
    11461 attgccacgc gctcggtagt agtcgccgac gggcgaatcg cctggcaggg tatgaccaat
    11521 gagttgttga gcggtaaggc gagtgcttcg gcgctgttgg gaattacagg ttaatatgca
    11581 ctgtcggatg cggcgtgaac gccttatccg acctacgggt gcgttttgtc cgcatcaagc
    11641 gccccacaca agactatgcc ctaaactcca accactttac gcaaaatatc gatatacacc
    11701 ggcattaacg gatggcgaac taacaccacc agcgccacca cgccgatggc agaaatcaac
    11761 ggcgtcagcc acaacaaacg accgcgggac aaataatgac tcaaacgatc ggttgctttg
    11821 ccgctgcgcc ataaccgcca gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc
    11881 agcagcaacc atttaaactc accgctctgc attccggctg gaatatcgat cgccgcaccc
    11941 gccagaatcc ccggcaggaa gtaaaacggt ggccacagca ggcagccgat aatattcggc
    12001 gtgataaatt ttgccaccgg gagatccagc attcccgcca ccattggcac cagcggacgc
    12061 gtcgggccaa caaaacgacc gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt
    12121 tcagttttat cgagtaatgc tttgttcttc ttcagaaatg accaacgatg caatggcttt
    12181 ttaaaacgcc agcccagcca gaaggagatc cagtcgccca gcagacaacc gacgatccct
    12241 gccagccagg cgtgccagaa acttaactcg ccgctgccaa tcagcgctcc cagccccgcc
    12301 atcagcaccg taccgggtag aatcaaaccg accagcgcca gcgactccag aaaggccacc
    12361 aacaccaccg ccatcaatga atacacggtg gattgggtaa taaagtgttc cagcaatgct
    12421 tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt
    12481 caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac
    12541 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
    12601 tcgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
    12661 ccgggtagtg ctcaaaagca gcttcgcctg gctgatgcgt tggtcctcgc gccagcttaa
    12721 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaggcaaac
    12781 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
    12841 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat
    12901 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
    12961 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
    13021 atccgggcga aagaaacccg tattggcaaa tattgacggc cagttaagcc attcatgcca
    13081 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
    13141 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa
    13201 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
    13261 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccattggcct caatcggcgt
    13321 taaacccgcc accagatggg cgttaaacga gtatcccggc agcaggggat cattttgcgc
    13381 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat
    13441 cagatattgc cgtcactgcg tcttttactg gctcttctcg ctaacccaac cggtaacccc
    13501 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa
    13561 aagtgtctat aatcacggca gaaaagtcca cattgataat ttgcacggcg tcacactttg
    13621 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca
    13681 actctctact gtttctccat acccgttttt ttggatggag tgaaacgatg gcgattgcaa
    13741 ttggcctcga ttttggcagt gattctgtgc gagctttggc ggtggactgc accaccggtg
    13801 aagaaatcgc caccagcgta gagtggtatc cccgttggca aaaagggcaa ttttgtgatg
    13861 ccccgaataa ccagttccgt catcatccgc gtgactacat tgagtcaatg gaagcggcac
    13921 tgaaaaccgt gcttgcagaa cttagcgtcg aacagcgcgc agctgtggtc gggattggcg
    13981 ttgacagtac cggctcgacg cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc
    14041 gcccggagtt tgccgaaaac ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg
    14101 ttgaagaagc ggaagagatt acccgtttgt gccacgcgcc gggcaatgtt gactactccc
    14161 gctatattgg cggtatttat tccagcgaat ggttctgggc aaaaatcctg catgtgactc
    14221 gccaggacag cgccgtggcg caatctgccg catcgtggat tgagctgtgc gactgggtgc
    14281 cagctctgct ttccggtacc acgcgcccgc aggatattcg tcgcggacgt tgcagcgccg
    14341 ggcataagtc gctatggcac gaaagctggg gcggcctgcc gccagccagt ttctttgatg
    14401 agctggatcc gatcctcaat cgccatttgc cttccccgct gttcactgat acctggactg
    14461 ccgatattcc ggtgggcacc ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa
    14521 gcgtggtgat ttccggcggc gcgtttgact gccatatggg cgcagttggt gcaggcgcac
    14581 agcctaacgc actggtaaaa gttatcggta cttccacctg cgacattctg attgccgaca
    14641 aacagagcgt tggcgagcgg gcagtgaaag gtatttgcgg tcaggttgat ggcagcgtgg
    14701 tgcctggatt tatcggtctg gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt
    14761 tcggtcgcgt actcggctgg ccgctggaac agcttgccgc ccagcatccg gaactaaaag
    14821 agcaaatcga cgccagccag aaacaactgc ttccggcgct gaccgaagca tgggccaaaa
    14881 atccgtctct ggatcacctg ccggtggtgc tcgactggtt taacggccgc cgcacaccga
    14941 acgctaacca acgcctgaaa ggggtgatta ccgatcttaa cctcgctacc gacgctccgc
    15001 tgctgttcgg cggtttgatt gctgccaccg cctttggcgc acgcgcaatc atggagtgct
    15061 ttaccgatca ggggatcgcc gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa
    15121 accaggtcat tatgcaggcc tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct
    15181 ctgaccaatg ctgtgcgctc ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg
    15241 cagacatccc atcagcccag caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc
    15301 gcagcgaaca ggcacaacgc tttgaacagc tttatcgccg ctatcagcaa tgggcgatga
    15361 gcgccgaaca acactatctt ccaacttccg ccccggcaca ggctgcccag gccgttccga
    15421 ctctataagg acacgataat gacgattttt gataattatg aagtgtggtt tgtaattggc
    15481 agccagcatc tgtatggccc ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt
    15541 gtgaaagcgc tgaatacgga agcgaaactg ccctgcaaac tggtactgaa accgctgggc
    15601 accacgccgg atgaaatcac cgctatttgc cgtgatgcta attacgacga tcgttgcgct
    15661 ggtctggtgg tgtggctaca caccttctcc ccggccaaaa tgtggatcaa cggcctgacc
    15721 atgctcaaca aaccgttgct gcaattccac acccagttca acgcggcgct gccgtgggac
    15781 agcatcgata tggactttat gaacctgaac cagaccgcgc acggcggtcg cgagttcggc
    15841 ttcattggcg cgcgtatgcg tcagcaacat gccgtggtta ccggtcactg gcaggataaa
    15901 caagcccatg agcgtatcgg ctcctggatg cgtcaggcgg tatctaaaca ggatacccgt
    15961 catctgaaag tctgccgatt tggcgataac atgcgtgaag tggcggtcac cgatggcgat
    16021 aaagttgccg cacagatcaa gttcggtttc tccgtcaata cctgggcggt tggcgatctg
    16081 gtgcaggtgg tgaactccat cagcgacggc gatgttaacg cgctggtcga tgagtacgaa
    16141 agctgctaca ccatgacgcc tgccacacaa atccacggcg aaaaacgaca gaacgtgctg
    16201 gaagcggcgc gtattgagct ggggatgaaa cgtttcctgg aacaaggtgg cttccacgcg
    16261 ttcaccacca cctttgaaga tttgcacggt ctgaaacagc ttcctggtct ggccgtacag
    16321 cgtctgatgc agcagggtta cggctttgcg ggcgaaggcg actggaaaac tgccgccctg
    16381 cttcgcatca tgaaggtgat gtcaaccggt ctgcagggcg gcacctcctt tatggaggac
    16441 tacacctatc acttcgagaa aggcaatgac ctggtgctcg gctcccatat gctggaagtc
    16501 tgcccgtcga tcgccgcaga agagaaaccg atcctcgacg ttcagcatct cggtattggt
    16561 ggtaaggacg atcctgcccg cctgatcttc aatacccaaa ccggtccagc gattgtcgcc
    16621 agcttgattg atctcggcga tcgttaccgt ctactggtta actgcatcga cacggtgaaa
    16681 acaccgcact ccctgccgaa actgccggtg gcgaatgcgc tgtggaaagc gcaaccggat
    16741 ctgccaactg cttccgaagc gtggatcctc gctggtggcg cgcaccatac cgtcttcagc
    16801 catgcgctga acctcaacga tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg
    16861 gtgattgata acgacacccg cctgccagcg tttaaagacg cgctgcgctg gaacgaagtg
    16921 tattacgggt ttcgccgcta agtagccgca tccggtatgt aacgcctgat gcgacgctgg
    16981 cgcgtcttat caggcctaca cgctgcaatt ttgtaggccg gataagcaac gcgcatccgg
    17041 cattcagcgc ctgatgcgac gctgacgcgt cttatctggc ctacacgctg cgattttgta
    17101 ggccggataa gcaacgcgca tccggcacga aggagtcaac atgttagaag atctcaaacg
    17161 tcaggtatta gaagccaacc tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg
    17221 caacgtcagc gccgttgatc gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga
    17281 ttacagcatc atgaccgctg acgatatggt cgtggtcagc atcgaaaccg gtgaagtggt
    17341 tgaaggtgcg aaaaagccct cctccgatac gccaactcac cgactactct atcaggcatt
    17401 cccgtccatt ggcggcattg tgcacacgca ctcgcgccac gccaccatct gggcgcaggc
    17461 gggtcagtcg attccagcaa ccggcaccac ccacgccgac tatttctacg gcaccattcc
    17521 ctgcacccgc aaaatgaccg acgcagaaat caacggcgaa tatgagtggg aaaccggtaa
    17581 cgtcatcgta gaaaccttcg aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct
    17641 ggtccattct cacggcccat ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc
    17701 catcgtgctg gaagaggtcg cttatatggg gatattctgc cgtcagttag cgccgcagtt
    17761 accggatatg cagcaaacgc tgctggataa acactatctg cgtaagcatg gcgcgaaggc
    17821 atattacggg cagtaatgac tgtataaaac cacagccaat caaatgaaac caggctataa
    17881 tcaagcctgg ttttttgatg gattttcagc gtggcgcagg caggttttat cttaacccga
    17941 cactggcggg ataccccgca aggaacagag gtctccttct ggctggcgac ggacaacggg
    18001 ccgttgcagg ttacgcttgc accgcaagag tccgtggcgt ttattcccgc cgatcaggtt
    18061 ccccgcgctc agcatatttt gcggggtgaa caaggctttc gcctgacgcc gctggcgtta
    18121 aaggattttc accgccagcc ggtgtatggc ctttactgtc gcgcccatcg ccagttgatg
    18181 aattacgaaa agcgcctgcg tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg
    18241 ccagaacgct atctgatgga gcggtttatc acctcaccgg tgtgggtcga gggtgatatg
    18301 cacaatggcg ctatcgttaa tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc
    18361 aagtgggttt ctattgatat tgaaaccacc cgccacggag agctgtactg catcggcctg
    18421 gagggctgcg ggcagcgcat cgtttatatg ctgggaccag agaatggcga cgcctccgcg
    18481 ctcgattttg aactggagta cgtcgccagc cgcccgcagc tactggaaaa actcaacgcc
    18541 tggtttgcaa actacgatcc tgatgtgatc atcggttgga acgtggtgca gtttgatctg
    18601 cgaatgctgc aaaaacatgc cgagcgttat cgtattccgc tgcggctggg gcgcgataac
    18661 agtgagctgg agtggcgcga gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa
    18721 ggtcggctaa ttatcgacgg tatcgaggcg ctgaaatccg cgttctggaa tttctcttca
    18781 ttctcgctgg aaactgtcgc tcaggagcta ttaggcgaag gaaaatctat cgataacccg
    18841 tgggatcgaa tggacgaaat tgaccgccgt ttcgccgaag ataaacctgc gctggcaact
    18901 tataacctga aagattgcga gctggtgacg cagatcttcc ataaaactga aatcatgccg
    18961 tttttgctcg aacgagcaac ggtgaacggc ctgccggtgg atcgacacgg cgggtcggtg
    19021 gcagcgtttg gtcatctcta ttttccccgt atgcatcgcg ctggttatgt cgcgcctaat
    19081 ctcggcgaag tgccgccgca cgccagccct ggcggctacg tgatggattc gcgacctggg
    19141 ctttatgatt cggtgctggt gctggactac aaaagcctgt acccgtcgat catccgcacc
    19201 tttctgattg atcccgtcgg gctggtggaa ggcatggcgc agcctgatcc agagcacagc
    19261 accgaaggct ttctcgatgc ctggttctcg cgagaaaaac attgcctgcc ggagattgtg
    19321 actaacatct ggcacggacg cgatgaagcc aaacgccagg gtaacaaacc gctgtcgcag
    19381 gcgctgaaaa tcatcatgaa tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc
    19441 ttcgatccgc ggctggtatc gtcgatcacc atgcgtggtc atcagatcat gcggcaaacc
    19501 aaagcgttga ttgaagcgca gggctacgac gtgatctacg gcgataccga ctcaacgttt
    19561 gtctggctga aaggcgcaca ttcggaagaa gaagcgacga aaatcggtcg tgcactggtg
    19621 cagcacgtta acgtctggtg ggcagaaacg ctgcaaaaac aacagctgac cagcgcactg
    19681 gaactggagt atgaaaccca tttctgccgt tttctgatgc caaccattcg cggagccgat
    19741 accggcagta aaaagcgtta tgccgggctg attcaggagg gcgacaaaca gcggatggtg
    19801 tttaaagggc tggaaaccgt gcgtacggac tggacgccgc tggcccagca gtttcagcag
    19861 gagctttacc tgcgtatctt ccgcaacgag ccatatcagg agtacttccg cgaaaccatc
    19921 gacaaactga tggcgggtga actggatgcg cggctggttt accgtaaacg ccttcgccgt
    19981 ccgctgagcg aatatcagcg taatgttccg cctcatgtac gcgccgctcg tcttgccgat
    20041 gaagaaaacc aaaagcgcgg tcgccccttg caatatcaga accgcggcac cattaagtac
    20101 gtatggacca ccaacggccc ggagccgctg gactaccaac gttcaccact ggattacgaa
    20161 cactatctga cccgccagct acaacccgtg gcggagggaa tactcccttt tattgaggat
    20221 aattttgcta cacttatgac cgggcaactt gggctatttt gagcaaaaaa agagttcgcc
    20281 agataccatt ttgatgcgtg acgaatgctt tgccatccag taccatagcg ccctttccat
    20341 tcctggacct gaataacacc actacctcat aagcacggta gcgggtggtt attgcctgca
    20401 attaaagata tagagccgaa cacatatgcc ttttacactt ggtcaacgct ggatcagcga
    20461 tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg gatgcgcgaa ctgtcacttt
    20521 acttttccca tctactggtg aaaaccgtct gtacgcacgc agtgattccc ccgtgacccg
    20581 cgtgatgttc aaccctggtg ataccattac cagccatgac ggctggcaga tgcaagtcga
    20641 agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt actcgcctgg atactgaaga
    20701 gtccggcgta gccctgcgtg aagttttcct tgatagcaaa ctggtgttca gcaaaccgca
    20761 ggaccgcctg tttgccgggc agattgaccg tatggaccgc tttgcgctgc gttatcgcgc
    20821 gcgtaagtat tccagcgaac agttccgtat gccgtacagc ggcctgcgcg gtcagcgtac
    20881 cagcctgatc ccgcaccagc tcaacatcgc tcatgatgtt ggccgccgcc acgcgccgcg
    20941 cgtcctgctg gctgacgaag tgggtttagg gaaaaccatt gaagccggga tgatccttca
    21001 tcagcaactg ctctctggcg ctgctgaacg tgtgctgatt atcgtcccgg aaaccttaca
    21061 gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg cgctttgcgc tgtttgatga
    21121 tgagcgttat gccgaagctc agcacgatgc ttacaacccg ttcgacaccg aacagctggt
    21181 gatttgctcg ctggattttg cccgccgtag caaacagcgt ctggaacatc tctgtgaagc
    21241 cgagtgggac ctgctggtgg tcgatgaagc gcatcacctg gtgtggagcg aagacgcgcc
    21301 gagccgcgaa tatcaggcta ttgaacaact ggcagagcac gtgccgggcg ttctgctgct
    21361 gaccgcaacc ccggaacagc tgggaatgga aagccacttc gcccgtctgc gtctgctgga
    21421 cccgaaccgt ttccacgatt tcgcgcaatt cgttgaagag cagaaaaatt atcgtccagt
    21481 agcggacgcc gttgccatgc tgctggcagg taacaaactg agcaatgacg aactgaacat
    21541 gcttggcgag atgatcggcg agcaggatat cgagccgctg ttgcaagcag caaacagcga
    21601 cagcgaagat gcccagagcg cccgtcagga gctggtttcg atgctgatgg atcgccacgg
    21661 caccagccgc gtgctgttcc gtaacacccg taacggtgtg aaaggcttcc cgaaacgcga
    21721 gctgcacacc attaagctgc cgctaccgac gcagtatcag acggctatta aagtctccgg
    21781 cattatgggc gcacgtaaaa gtgcggaaga ccgcgcccgc gatatgctct acccggagcg
    21841 tatttatcag gaatttgaag gtgataacgc cacctggtgg aacttcgatc cgcgcgttga
    21901 gtggctgatg ggctacctga ccagccatcg ctctcagaaa gtgctggtga tctgcgctaa
    21961 agctgccact gcgctgcaac tggagcaagt actgcgcgaa cgtgaaggta ttcgcgctgc
    22021 ggtgttccac gaaggtatgt cgattatcga acgtgaccgc gctgccgcct ggtttgccga
    22081 agaagacacc ggcgcacagg tactgctgtg ctcagaaatc ggttctgaag gacgtaactt
    22141 ccagttcgcc agccacatgg tgatgtttga cctgccattc aacccggatc tactggagca
    22201 gcgtattggt cgtctggatc gtatcggcca agcgcacgat attcagatcc atgtgcctta
    22261 tctggagaaa accgctcagt cggtgctggt gcgctggtat cacgaaggtc tggatgcatt
    22321 tgagcacacc tgcccgaccg gacgcactat ttacgatagc gtatacaacg atctgattaa
    22381 ctatctggct tcaccggatc aaaccgaagg ctttgacgat ctgatcaaaa actgccgcga
    22441 gcaacatgaa gcgctgaaag cacagctgga acagggtcgt gaccgcctgc tggaaatcca
    22501 ctccaacggt ggcgaaaaag cccaggcact ggcagaaagc attgaagagc aggatgacga
    22561 taccaacctg atcgccttcg ccatgaacct gttcgatatt atcggtatca atcaggacga
    22621 tcgcggcgac aacatgatcg tgctgacgcc gtccgatcat atgctggtgc cggacttccc
    22681 tggcctgtcg gaagatggca tcaccatcac ctttgatcgt gaagtggcgc tggcgcgtga
    22741 agatgcgcag tttattacct gggaacatcc gctgatccgc aacggtctgg atctgattct
    22801 ttctggcgat accggtagca gcacgatttc actgttaaaa aacaaagcgt tgccggtagg
    22861 tacgctgttg gtggaactga tttatgtggt tgaagcccag gctccgaagc agttgcagct
    22921 caaccgcttc ctgccaccga cgccggtacg tatgctgctg gataaaaacg gcaacaacct
    22981 ggcggcgcag gtagagtttg aaacctttaa ccgccagctt aacgcggtta accgtcacac
    23041 cggcagcaaa ctggttaacg ccgtgcagca ggatgttcac gctatccttc aactgggtga
    23101 agcgcagatc gagaaatctg cccgtgcatt gattgatgca gcacgtaacg aagccgacga
    23161 aaaactgtct gccgagctgt ctcgtctgga agctctgcgt gcagtgaacc cgaacattcg
    23221 tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag gtaatggaaa gcctggatca
    23281 ggcaggttgg cgtctggatg ccctgcgttt gatcgttgta acgcatcagt aacggagccg
    23341 aaaatgggga tggaaaacta caatccaccg caggaaccct ggttggttat cctgtatcag
    23401 gatgaccata ttatggtggt caacaagccg agcggtttgt tgtctgtgcc gggtcgtctg
    23461 gaagagcaca aagacagcgt gatgacgcgc attcagcgag attatccgca ggcagaatcg
    23521 gtgcatcgtc tggatatggc taccagcggc gtgattgtag tggcgctgac caaagccgcg
    23581 gagcgggagt taaaacgcca gttccgcgag cgcgtgccga aaaagcagta tgtgacccgc
    23641 gtctgggggc atccatcccc cgcagaaggt ctggtggatc tgccgctgat ttgcgactgg
    23701 ccaaaccgcc cgaaacagaa agtctgttac gaaacgggta aacctgcgca gacggaatat
    23761 gaagtggtgg agtatgcggc ggataacacg gcaagagtgg tgttaaaacc gattaccggg
    23821 cgttcgcatc aattgcgtgt gcatatgctg gcgctggggc atccgattct cggcgatcgt
    23881 ttttatgcat caccagaagc gagagcgatg gcaccgcgtt tgttgctaca tgcagagatg
    23941 ttgacgatta cccatccggc gtatggcaat agtatgacgt ttaaagcgcc agcggatttt
    24001 taacctttcc tgtacgttta taccctcatc ctaagggctg cctcttgtac ataaatcccc
    24061 gagtccgggg attttttgtt tctgccaaac tgctttcagt tgttgcgtgt ggctacgctt
    24121 tccattcggt aattcccttc ctgctccaca gcgaattttt gaactttttc ttcacccgtt
    24181 gaccgtctct tatatttcat tttatcgggt ctgttatggt cctttctgat tgttattcat
    24241 gggctaatta gcagtctggt tggcgatgcc catctcggtg atccgcgtcg tacacgacgc
    24301 cttgcgtcac tagcctcttc tctagctcat catgcaggtc tttctaattg tgaaatcatc
    24361 ccacgctact gctaaggttg aaggggcgtt ccagcccctt caaccttagc ttattctggc
    24421 tcattcaata attgcgttgt cagtaatttg ttcagcagta ctttgtttag tagcaacttc
    24481 ttcagtatca atttttttag gggtgatttt attaataata attacctcat catataggtg
    24541 ggtagcaccg tccagggtag caccgtccag gttagcattc cacccaggtt ccaagtaaag
    24601 gactttattg tgacctttca ttgttttgag accaaggtaa caatccctca tgtcaacgcc
    24661 gggaaggtaa gcctctctaa gatcaacgcc caagttacct gggcctttat gcagctgcaa
    24721 cgtctgagta catatctcct tattttcaat aaaaaaagtg acacttaact cgatatcacc
    24781 gaaggtctgt gcgacccagc tcatattgcc ctttgcgacc atctcatccg gattttttgc
    24841 gcatgttttg agttcattga ataattccag actatggata aacttttctt tctcattctc
    24901 gttgacagcc ccagtaaagt attttgaggc gttgatgttg ccagcctttt tattcacgaa
    24961 attgaacaga agtttataag cctcagtctg tttatctgac cagaacagat gacgtaacgt
    25021 ctcccacaag cgttgaaaga tattcatctt catgtccatc ttgtccccgc tcaccatctt
    25081 ataaatatcg ttattgctca tatttaaaag tgtagccggc atgcctggaa ctgatacttt
    25141 catggtaata gcctcgcatt gagttaaaaa ctcatgatgc gcgctctgaa taaatatact
    25201 ttcaaaaatc gctcattcag atatgtgttt tttattttta agacgtaaaa atataattcc
    25261 gctcgccgta aagctctcaa ccttaagcag ggtcaacgcg ataaaaccaa cagaaaccct
    25321 cccgtaaacc gtatattgag cctgtccacc attctgagta aacgcctttt ctcaaagaag
    25381 tgaccgccag ggcggccacc ttttatcgtg ggcagttact cagcatcatt tacagggtca
    25441 tttaaaccct ttctgctgct taatcagctc ataggcctgc tgaatttcct gcgctttctg
    25501 cttcgccatc tccatcatct caggcggcaa acctttcgcc accagcttat cgggatggtg
    25561 ttcactcatc agcttacggt aggcacgttt gatggtggtc gcatcatccg tcggcttcac
    25621 gcccagcaca ttacaggcat cttccagcgt tgggccacgc tgcgcttgct gccagttacc
    25681 accgccagat tgctgctgat aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa
    25741 aaactggtca aactgggcgc gggagatccc taattcttca gcaatgacat acagcaccgc
    25801 ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc tgaatctgga tctccagaaa
    25861 catacgaatt aagtcaaaac gaccaaagca gacactgcga aactggcgca tcttttcgcg
    25921 cagcgggtaa ttgtctgatt ttcccacccg gaacgcattt tgcgccgcag tacgggaagc
    25981 gccatgaaga ttcattcggt ccatcaactg gctggcgata tgaatatcag cctccgtgac
    26041 gcgacctttg gatttggtta aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc
    26101 ctgacgctca cgctggttgg cgaaccacgc cattttacgg ctacgggctt tatcaaacat
    26161 atggccaatt aacaggccta acactacgcc ccaaaagccg ccgcccatca gtaaggccac
    26221 ggccacgcca atgatttttc cccaatactg catatattcc ccaaatcgac acacggatat
    26281 cagggctatc tcccacaata taaaggtgct tttaccgttt tccggctgcg gtcaattgtg
    26341 ggacatcgcc tataatttgc attatcatac ctgtcattca atgccgtgcc taacaccact
    26401 gacgctattc ggacaggatt aacactagcg tagagatgac gagtacgtta gtctctgacc
    26461 gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta
    26521 tccccactct cctggccacc atgattgcca ccgcccttta tagtcaacag ggactggcag
    26581 ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct ctggtacagg
    26641 gcgataccaa tgacttaccc gtgactatca atgctaacca cgcgaaaggg gactacccgg
    26701 atgacgccgt gtttactggc agcgtggata tcatgcaggg taacagccgt ctgcaggccg
    26761 acgaagtgca gctccatcaa aaagaggcac caggacaacc ggagccggta cgtaccgttg
    26821 atgcgctcgg taatgtccat tacgacgata accaggtgat cctcaaaggg ccgaaaggct
    26881 gggcgaatct gaacaccaaa gataccaacg tctgggaagg tgattaccag atggtgggtc
    26941 gccagggtcg cggtaaagcg gacctgatga aacaacgtgg cgaaaaccgc tataccattc
    27001 tggataacgg tagctttacc tcctgtctgc cgggttctga cacctggagc gtggtaggta
    27061 gcgaaattat tcatgaccgc gaagaacaag ttgcggagat ctggaacgcc cgctttaagg
    27121 tgggtccggt atcgatcttt tatagcccct atttgcagtt gccggtgggt gacaaacgtc
    27181 gctctggttt cttgatcccg aacgccaagt acaccaccac caactacttt gagttctacc
    27241 tgccatatta ctggaacatc gcgccaaata tggatgccac catcacgccg cattatatgc
    27301 atcgtcgtgg caacatcatg tgggagaacg aattccgcta cctctcccag gcgggcgctg
    27361 gcttgatgga actggactat ctgccttcag ataaagtcta tgaagatgaa cacccgaacg
    27421 atgacagttc acgtcgttgg ttattctact ggaaccactc cggggtcatg gatcaggtgt
    27481 ggcgtttcaa cgtcgactac accaaggtca gcgatcctag ctacttcaat gatttcgata
    27541 acaagtacgg ttccagtact gacggctacg caacgcaaaa attcagcgtt ggctatgcgg
    27601 tgcaaaactt caatgccacc gtttcaacca agcagttcca ggttttcagc gaacagaaca
    27661 ccagtagcta ctcggcagag ccgcagttag acgttaatta ctaccagaat gatgttggtc
    27721 cgtttgatac gcgtatttac ggccaggcag tgcactttgt taacaccaga gacgacatgc
    27781 ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt gccgctctct aataactggg
    27841 gcagcatcaa taccgaagcg aagttgctgg caacccatta tcagcaaacc aatcttgact
    27901 ggtataactc cagaaacacg accaagctgg acgaatccgt taaccgcgta atgccgcaat
    27961 tcaaagttga cggcaaaatg gtctttgaac gcgatatgga aatgctggct ccgggttata
    28021 cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca
    28081 tctataacta cgactcgtct ctgctgcaat ctgactactc tggcctgttc cgggaccgga
    28141 cttacggcgg tcttgaccgt attgcctccg ctaaccaggt gacgaccggt gtcacatctc
    28201 gcatatatga tgatgctgcc gttgaacgtt ttaatatttc cgttggtcaa atctactatt
    28261 tcacggagtc tcgcactggc gatgacaaca taacatggga gaatgacgac aaaacgggtt
    28321 cactggtgtg ggcaggcgat acttactggc gtatctccga gcgttgggga ttgcgtggcg
    28381 ggattcagta cgatacacgt ctggataacg tagcgaccag taactccagc attgaatacc
    28441 gtcgggatga agaccgtctg gtacagctga attaccgtta cgccagcccg gaatatattc
    28501 aggctacgct gcctaagtac tattccactg ctgagcaata taagaatggt atttcgcagg
    28561 taggtgctgt cgccagctgg ccaattgccg atcgttggtc cattgttggg gcctactact
    28621 acgacaccaa tgcgaacaag caagccgact ctatgttagg tgtgcaatac agctcctgct
    28681 gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg ttgggataac gataaacaac
    28741 atgcggtata tgacaacgca atcggcttta acatcgaact tcgcggcctg agctccaact
    28801 acggtctggg tacgcaagag atgctgcgtt cgaacattct gccgtatcaa aacactttgt
    28861 gatttgttga tttaccacgt aatccgcagt gcggttaatt gaaatggaaa aagtatgaag
    28921 aactggaaaa cgctgcttct cggtatcgcc atgatcgcga ataccagttt cgctgccccc
    28981 caggtagtcg ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt
    29041 gatggattaa tgcagtcggt aaaactgaac gctgctcagg caaggcagca acttcctgat
    29101 gacgcgacgc tgcgccacca aatcatggaa cgtttgatca tggatcaaat catcctgcag
    29161 atggggcaga aaatgggagt gaaaatctcc gatgagcagc tggatcaggc gattgctaac
    29221 atcgcgaaac agaacaacat gacgctggat cagatgcgca gccgtctggc ttacgatggt
    29281 ctgaactaca acacctatcg taaccagatc cgcaaagaga tgattatctc tgaagtgcgt
    29341 aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg aagtcgaatc cctggcgcag
    29401 caggtgggta accaaaacga cgccagcact gagctgaacc tgagccacat cctgatcccg
    29461 ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt
    29521 gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca ttctgccgac
    29581 cagcaggcgc tgaacggcgg ccagatgggc tggggccgta ttcaggagtt gcccgggatc
    29641 ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc
    29701 gttggcttcc atatactgaa agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg
    29761 accgaagttc atgctcgcca tattctgctg aaaccgtcgc cgatcatgac tgacgaacag
    29821 gctcgtgtga aactggaaca gattgctgct gatatcaaga gtggtaaaac gacttttgct
    29881 gccgcagcga aagagttctc tcaggatcca ggctctgcta accagggcgg tgatctcggc
    29941 tgggctacac cagatatttt cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa
    30001 ggtcaaatga gtgcaccggt tcactcttca ttcggctggc atttaatcga actgctggat
    30061 acccgtaatg tcgataaaac cgacgctgcg cagaaagatc gtgcataccg catgctgatg
    30121 aaccgtaagt tctcggaaga agcagcaagc tggatgcagg aacaacgtgc cagcgcctac
    30181 gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt tgtgatcact cccggcgagc
    30241 ccgccgggat tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac
    30301 tggttgtttg tgccgatgcc actctcctta ccgaccgggc agcgatgctc ggtttgccgc
    30361 tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactacg ggcacattaa
    30421 cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg gcagttagcg attgaaaatg
    30481 ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg
    30541 ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa cgacgcaggc attccgttta
    30601 ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa aaaagtggtg atgatgctgg
    30661 cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc gatatcgcag
    30721 atgctatcac ccctgcactt ttgcacgaag tgattgctat tttgcatcac gatttgcgta
    30781 caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg
    30841 aaggcggtca tatgggtacg gaagagatag acaccattat tcctgtgctc gacgagctgc
    30901 gggcgcaggg gatgaaactc aacgggccgc tgcctgccga taccctgttt cagccgaaat
    30961 atctcgataa cgccgacgcc gtgctggcga tgtaccacga tcagggtctt cccgtgctaa
    31021 aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt attcgcacat
    31081 cagtggacca cggcaccgca cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt
    31141 ttattacggc gcttaatctc gccatcaaaa tgattgttaa cacccaatga ataatcgagt
    31201 ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt
    31261 gatcgacagc attgtttctg ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg
    31321 ccccggtctg gcggcattga ccgagccggt cggcgaacgt ctggaccagc tgacggtcat
    31381 cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat ccattcttag gcccgaaact
    31441 gacgatttat cagcaggatg cgatgacctt taactttggc gaactggccg agaaaatggg
    31501 tcagccgctg cgtgttttcg gcaacctgcc ttataacatc tccacgccgt tgatgttcca
    31561 tctgtttagc tatactgatg ccattgccga catgcacttt atgttgcaaa aagaggtggt
    31621 gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt cgattaagcg tcatggcgca
    31681 atactattgc aacgtgatcc cggtactgga agttccgccg tcagccttta caccaccacc
    31741 caaagtggat tccgccgtcg tgcgcctggt tcctcatgca acgatgcctc acccggttaa
    31801 agatgttcgt gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc gtaaaaccat
    31861 tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc
    31921 ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc cagatggcga actatctggc
    31981 ggagaacgcg ccttttcagg agagttaacg atgatcaatt cgccccgagt gtgtattcag
    32041 gttcaaagcg tctacattga ggcacaatct tcacctgata atgaacgtta cgtttttgct
    32101 tataccgtaa ccatacgcaa tctggggcga gcgccagtgc agttgttggg gcgttactgg
    32161 ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc
    32221 cagccactta tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat cattgaaacc
    32281 ccgctgggca ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt ccctttcagc
    32341 atcgacattc ccgtattccg actcgccgtt cccacactca ttcattaaaa gaatatggcg
    32401 acatacctta ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt gctgcataaa
    32461 gtagaattta cccctgggaa agataccctc tggctgacgg gcgatctggt cgcgcgcggc
    32521 ccgggttcgc tggatgttct gcgctatgtg aaatccttag gcgacagcgt acgtctggtg
    32581 ctgggcaatc acgatctgca tctgctggca gtatttgccg ggatcagccg caataaaccg
    32641 aaagatcgcc tgacaccgct gctggaagcg ccggatgccg acgagctgct taactggctg
    32701 cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc tggtgatggc ccacgcaggg
    32761 atcacgccgc agtgggatct gcagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg
    32821 ctgtcgagtg actcctatcc cttctttctc gacgctatgt acggcgatat gccaaataac
    32881 tggtcaccgg aattgcgggg gctgggaaga ctgcggttta tcaccaacgc ctttacccgt
    32941 atgcgttttt gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc gccggaagag
    33001 gcccctgccc cactgaaacc gtggtttgcg attcctggac ctgtcgctga agaatacagc
    33061 atcgcctttg gtcactgggc atcgctggaa ggcaaaggta cgccggaagg tatttacgcg
    33121 ctggataccg gctgctgctg gggtgggtca ttaacctgcc tgcgctggga agataaacag
    33181 tattttgtcc agccgtcgaa ccggcataag gatttgggtg agggagaggc ggtcgcgtct
    33241 taaatcccgt cgagcataaa atctaatgct cctttaatca acgttcggtg cgcgctggca
    33301 tcgcaaaatt tcgttcccag cgcgttaact ggaacagttg cagtctcatg cgtcatgagc
    33361 acatattctt tgccgtctac cagtaaaagg attgggttaa gccgttctgg tgggcgaata
    33421 cggctaaatc gttcaatagg tgttaatgga ataacaatac ggcgattaat cacaccaata
    33481 atgtcgctgg taacatcgat aacaaaggga aacgcggcgt tcctgctgcg actgcggtat
    33541 accgtaaatt gcatcgctag aagctccggt actcatcaga gaaacatccg gtttcatcag
    33601 ccaattgatt taacgcagcg atagcttcag cgttctcttc acgccaacgt gttgctgcat
    33661 gttttttaag ttcagcattg agcgcaaccg taagggtggc gctcatattt aagcctgcat
    33721 cacgagcgcg attgactaac tcacggtcga cggtgacggt cacactttgt gtggtacgtt
    33781 ttgcagtcat gatatcgtgc tcctgtacaa tttatacgtc tagtatacac ggatatctgc
    33841 acgtatcagt gatgccggaa ttctaatata caaaattacc gccgctccag aatctcaaaa
    33901 caatagctgt gagagttctg cgcatcagcg tcgtggaatt cgctgaatac cgattcccag
    33961 tcatccggct cgtaatccgg aaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc
    34021 aggtacagtt tttgcgcttt cggcaggaac tgctcataaa cgcgaccgcc gccaatcacc
    34081 atgatttctg gtacgtcacc acacgccgcg atggcttcat ccaccgactt cacccacgtt
    34141 acgcgatcgt ccgtacccgg ttgactgctg aggataatat ttttgcgtcc tggcaacgga
    34201 cgaccgatgg attccctggt atggcggccc ataattacgg gtttatttaa ggtgttgcgt
    34261 ttaaaccagg cgagatcggc aggcaggttc cacggcatgg cgttttccat gccgataacg
    34321 cgatctaccg ctaacgccgc aatcagactg atcattgaga tttcccgata aaaaaaattg
    34381 tcgccactat acgtaaagcg taaaccgtcg tcgactggtg cgaggatgat gttgaggaaa
    34441 attttatatt ctgctggcga gtccacgctc tctccctgga ctcgccgcat tacaatgaaa
    34501 caaaaacaaa cagttagctg taaagtgtga tttacgtcac tctttattag gatgagggtt
    34561 tcgtttccgg ttcatccgcc atgttgccgg tatgtttacc ttcttctgtt ccctgccagc
    34621 catgtcgttg aattaatgac agatgttcgc ggtcctcggt gatgatctca cttaacatcg
    34681 cgctggtgcg tttatagacc gccgcgcggg ctttggtgtc attctcaacc attgccatct
    34741 cttccaccat ctgaatatta aagcggcgga acacatcggc acgttctcgc gcttcatacg
    34801 gccccagacc taaactttcc agcgccagac gcccggtttt cagcgcacct tcaaaggttt
    34861 cacgctctgg cttttcaacg cctgcctgac gcaaacaaat gtagtgatcg acatcgcggg
    34921 cgcgggcaat aatctgcaaa tgcgggaaat gttctttcac catctccgtc agttgcaggt
    34981 tggtttgcgg atcgtcaatg gcgttaatca gcacttccgc tttcgccgct cccgcagatt
    35041 ccagtaaatc catccgcgtg gcatcgccat aaaacacttt cataccaaat ttacgcagcg
    35101 tttcgatatg gtccggatcg tgatcgagca ccaccatttt caccccgctg gagagcagta
    35161 aacgtccggt gatctgcccg aaacgaccga atccggcaat gattacccgc ggctgttctt
    35221 cgtcgatctc atcggcttca cgcgcctctt cagtagaaga ttgctcaagg cgattgagga
    35281 tcaccagcag aatcggcgtt gccgccatcg acagcgccac cgccagggtc agcgatttcg
    35341 cccactccgg ctccagcaca ttcgccatct gcgccgcgcc aaataccaca aaggcaaact
    35401 cactgccctg ccctaacaac accgcaaacc agcgacgctg tttatttggc acctgcaacg
    35461 gtcgggcaat cagccacagc atggcgattt tgatgatgag gaaaccgagc agcaaaatga
    35521 caatgcgcaa tgggttttca atcagcgtgc caaagtctat cgacatgcca acaccgatga
    35581 aaaacagccc caacagcaaa cctttaaagg gttcgatatc gctttccagc gcatgacggt
    35641 attccgagct tgccagcagt acgcccgcca gaaacgcgcc catcgccatc gacaagccga
    35701 cctcttccag cagcaaacca aagccaaaca caaggaataa cgccacggca ctaaacactt
    35761 cccgcaagcc agagcgggca acaaaacgca gcgccggacg cgtgacatag cgccccagca
    35821 gtaccaccag caccagcgcg cccgccactt ttaacgccga gagagcaaat gcgcccatcg
    35881 tcgtcgaggc actgctcgcc gccagtagcg ggatcatcgc caccagcggg attgccgcga
    35941 tatcctggaa cagcagcacc gcaaaggcac tgcggcccat ttgcgtcacc atcagattgc
    36001 gttcattcat cgcctgcatg gcaatcgccg tagaggagag cgccagcgtc atgccgatca
    36061 attccgcgac ctgccagcgc agcccaagta acatacagaa cagccccagc aggccgccgc
    36121 aaatcaccat ctgcaatgca ccaccgccaa acactgccgc acgcagcttc cacagccttt
    36181 gtggatcgag ttcgaggccg ataataaaca gcatcagcac cacgccaatc tcggcaaagt
    36241 gcagaataga ttcggcatcg gtcaccagtc gcagccccca cgggccaata atgcagccgg
    36301 cgatcaggta gccaagtacc gatcccagac caagacgtac tgcaatgggt acaatcagcg
    36361 ccgccgaacc gagataaatc agcgcctgaa ccagcgtatg gctatccatg atgggcctcc
    36421 tgccattcca gcagacgttg cttatagtga cgcgcctgcc cttcgagggt ttcgtcgtca
    36481 caaataaagg tgcagtgcat ggcaaacggt ggcagccagt tcagcccgca gtagattgcc
    36541 gtcgcctgta gcggctgcga cagcacatca aagcccggat gcgcaccaat ttcaaaatgg
    36601 ctttccccgc cgccggtcgt caccgcccac agcaaatgtt tgccatgcag cgccgtgccg
    36661 ccgtgaccgt aagcccagcc gtgcgagaag actttatcga tccaaagttt aaggagcgga
    36721 ggaatgctgt accactgcat cgggtgctgc cagacgatca gatcggcgcg agacagcgcc
    36781 tcctgctcgg cggcaatatc gatattgaag tcaggataga gttgataaag agagcgaatt
    36841 tcgacgcctt ccagcgtcct tgcctgttca aacatccgtt tattcgcatg ggaatgatgc
    36901 ggatacggat gcgcataaat tataagaatc ataggtagcc cgcgtgttta cgcctttgtt
    36961 ttcccacaaa gtgtagtcat atattcacgc ggcttatagt caataatatt gattcagaaa
    37021 agagataaat ctgaacaaat taccccattc tctgcccgcg gatcgtcata ttgctggtct
    37081 gcgccagtga catccctcga gtctccgggg caaacgctac ggaaatcaac aagccaaaca
    37141 gcgagatacc cgcccccatt agcatcgtgt tactgatacc gtaattattg ataaagatcg
    37201 gtagtgccca ggtcgaaaca atggtgccaa tacgacttaa ggacataatc acgcccacgg
    37261 cagaggcgcg gatatccgtc gggaagagtt cattaggata gagccactgc aaattacccg
    37321 gcccgccaga gaaaaaggca tacaccgcaa aggccatcac taccagccag atccccatat
    37381 ccgggatcag ccccaaaacc gccagcgcca gcgtcatcat ggcaaagctg ccaatcaaca
    37441 atggacgccg tccggcagtg tttagccaca gcatcggcgg aatacagccg agcataaaga
    37501 acaggctaat caccacgttc cccagtgccg cgtttttgcc aacacccaat cccaacaaac
    37561 caacgatttg cgggccaaag gtgtaaatgg cgaacattgg gatcacctgg caggtccaga
    37621 tggcggcaac aaacagaaca aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg
    37681 tttgctgcgg ctgctcttca tcgaaagcca ccggttcgcc aaacagtttg atcatcatct
    37741 cttcgcactc ttttactcgc cctttgcgta ataaccagcg gggagattca ggcagttcga
    37801 atcgaccaat caaaatcaac aaacagggga tcgccgcgct acccagcatc cagcgccagc
    37861 cgccttccac atcataaagc cagtagccga ccagatcggc acaggtcgcg ccgacatacc
    37921 acatggcggc gataaagctg atggaaaacg cccgctgacg ggtactggag aactcagtga
    37981 tcatcgaagt ggcgatggga tagtctgcac cgatgacaat gccgataagt acccgcatca
    38041 ccaacagttc gacgggggat gaaacaaaca tcgtcgccac cgatatcacg ccgatggcga
    38101 tgatatcaat gaggaacatt ttgcgccgtc cgactttatc ggaaatataa ccaaacagcg
    38161 atgtgccaac gaacagcccg gcgagcgttc ccgcgcccag caagccaatc cagtcagcgt
    38221 ccagttttag cgccggcgtc agttgctcca gcgccacgcc aatcattacc agtatataac
    38281 catccagaaa cggaccaccg cttccccaca gcaaaatgcg gcggtgaata gaggaaaatt
    38341 taagatcgtc aaagtttctg gacggttgca tgggttattc ctgttttttg caatgagagt
    38401 cagtgcggcg ttacgtatca aaccaacatc agccgtaacg gaactccaca ccaaaggtgc
    38461 cgcgcgggta ttcccactgt tccagcgccg accccagccc cagaatgcga caggtgccgc
    38521 actccagaca tccggcgtaa tcgaagcgca cactgccgtc atcctgcttc ttgtacagac
    38581 ctgcggggca cgctttcacc agcagctcca gcgcctgttt atcggcatcg gctttcacaa
    38641 caatgtgcgg atgctcttca tcgacattga atttattgac gcccagtttg acatccacat
    38701 tgacgggaga agtcataaaa cggtcactcc tttcatgcca tccttgatca gattgatgaa
    38761 gcccactttc ttgccgtggc ggaggatttt cttgcgcatc agttccggcg cactgccgtc
    38821 aatggtgaac aggtcacgcg ccacgcctac cgccagttcc gggtagccgc taaacatgcg
    38881 tgggttatca aggaaggccg gtagtttctg gtacatacgc atatcgcgca gcgggccact
    38941 ctcaagatgc tgacgatatt ccgcgagttt ttgcttactg aaatcgtcgc ttttcatcgc
    39001 tgaaagcacg gtttttgctg cagcttcccc ggcggcaatc gccagatcca taccgcgaat
    39061 ggtaaaaccg aggttcatac acattccggc ggcatcaccg gcaatcaata cgccgtcacc
    39121 gaccagctcc ggcagcatgt tgatgcctgc ttccggcact acgtgagcgg aatattccac
    39181 cagcttgcca cccgcgatca gcggtgcaac ggccggatgt tgtttgaaat cttccagcat
    39241 ttgcggcacc gattttttcg cgtcatgcag atgatgcaga ccacaaacca gccccagcga
    39301 cagggtgttt tcattggtat aaagaaaacc gccgcccatc aggccatcgg tgggtgatcc
    39361 cgcaaatagg caagccgccc cctgattacc ctgcaactga aaacggtctt caataaccga
    39421 cttcggtaac tcgatcagtt ccttcacgcc aaccgccaca ttcgtcggtt taacgcgttt
    39481 tgccatcccc agcttttcgg caaggatgga gttcactcca tcagcaagga tcaccgtttt
    39541 cgcttcaatc acatcgccat cggcttctac accgacgact ttgccatcgc gctgtacgag
    39601 gttatcgacg cggatcccgg taattaactg cgcgcccgct tcttcggcct gctccatcag
    39661 ccaggcatca aatttactgc gcaaaacgga gtaagaacgc tgggatggcg aggtttcgtc
    39721 accgttgcag tagtccatag tcatcgctga cttttccgtc ataaacgcga gtttttcatg
    39781 ggtgatcagg cgttctaccg gggcggattc ggcgaaacca ggaataatgt gttccaggct
    39841 gtgggcatag agacgcccgc cggtgacgtt cttggcacct gcggaattgc cacgctcgat
    39901 aactaacact tgcgcccctt cgcgggcgag caccagtgcg gcaaccgaac cggcaagccc
    39961 tgcaccgacg atgatggcgt caaagatatc ttcggacata agagctccct gtcagcggca
    40021 ggaccaaaat gcagccctgc cagagtggat caacgcgcta aagctgcggt cagcgccgga
    40081 aggatcttca cggcgtcgcc aacaatgccg taatccgcgt actggaagat cggcgcattt
    40141 ttatctttat tgatggcgaa aatggtttgc gacgcgttag cgccaaccat gtgctggatc
    40201 tgcccggaga tccccacagc caggtacagt tcaggtttca gcatcaggtt ggagataccg
    40261 acatagcgtt cgtgctccat ccatttttcg ttttctgcca ccggacgaga acaggccaat
    40321 tccgcaccta tcgccttgca aagctgttct gccagcgcaa tgttctcttt gctgccaata
    40381 ccgcgaccga cgctgaccac caggcgggct ttgtcgagat cgacgctgtt gctctggcgc
    40441 gcctgggttg ccgtgcgggt aatcgccaca gccggagcct gccactccac ggtgtgcgtt
    40501 tcgccagtgc gtgacgcgtc tggctgagcc gcatcgaacg tgccgctgct gatggtcagt
    40561 accgcatacg gcgtggcaat gcgttcttcg ccaatcgcca gaccaccgta aaccatatgt
    40621 ttcactgttg ctttaccgtc ctgtacgctg acggtgctgg catcgttaga caccgccgct
    40681 ttaaggcgat aacccagttt tgccgccagt aatttgccgc gacgggtgtt tggcaacagc
    40741 accaggccgt ctgcaccgtg ctggcgaata gtgtcagcca tgacactggc gtaatcttcg
    40801 atcatccgat cgtccggttt gccgtttaat ttccagacat gattagcgcc gagctggatt
    40861 gcctgtgcgc cgtcggcatc attgaggaca aaggcattga tttgattagc taaagcctgc
    40921 gcaccgttca tcagttccgg cagacgagaa ggggtatcgc tgaataccca gacttgagaa
    40981 aacgtgttca tagcatcccc tgtaattaaa tgactttgcg aagattttca gcaaatgcgg
    41041 cgatctgttc ttcgccgtcg ccttcaatca cgatgcgctg acgctcgcgc tgtttcggcg
    41101 cggcaacctg ttgttctgac catgctgcct ctgcgttaaa accgatatcc gccgccgacc
    41161 atacctggac tggctttttc gccgcgccga gaatggcttt catcgaagga atttgtgggg
    41221 agttgatatc agtggaaaca gcaacaaccg caggcagtgg aatgcttaag gtttcggttt
    41281 catcttccag ttcgcgctca acggtgaggg tatctgccgt cagggagata attttgctga
    41341 cgccgttaac tgccggaata ttgaggattt cgcctaccag caaaccaacc tgctgggcat
    41401 aaaggtcgga agaaccatcg ccacagagga tcaaatcaaa gcctgctttc tgggcggctg
    41461 cagccagtgc gctcgccgtt tgttgcggca gtgcctgctc gaactggtca tcaatcacca
    41521 caatcagttc atccggaccg cgcgatagca catctttacg ccctttggcg ttggtcaggg
    41581 ctttaccgcc cacacttagg gccgtcacct gcgcctctgc tgcctgttgc tttagctggc
    41641 aagccgcttc aatagcgttg agatcgtatt ggcttatttt ggcatcggct ttactgaagt
    41701 ctaacgaacc atcagcatta ttgaccgcaa tatcctgttc atcaggcacg cacttatagc
    41761 aagtaataat cttcattgca tctccagaaa tcatgaagga aacgtcgttc atatcggtgg
    41821 tgttaattac agatatcatg ccctttaatt tggaaataaa acttcaatca ccaatattga
    41881 aaatgtcaca cgcagataaa tccaactttc aatattgtta agttcctcac caatattgaa
    41941 aacacggcgt agcaaaaaga aattttcaat attgttttat ggatcaccaa tattgaaagt
    42001 cagatgctat ttttttactc gcaacataac ataacaaact gattaataat caaaattaac
    42061 gagaaaagcg ccctctattc acccgaaaca aaaatgtgat aacaatcaca gaatgcagct
    42121 tattgaatac ccattatgag ttagccatta acgc
//
LOCUS       DAZFDC010000023        40121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFDC010000023 DAZFDC010000000
VERSION     DAZFDC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.023"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHJ48_001412"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHJ48_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO4707001.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2095..2961
                     /gene="yghU"
                     /locus_tag="ACHJ48_001413"
     CDS             2095..2961
                     /gene="yghU"
                     /locus_tag="ACHJ48_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO4707002.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHJ48_001414"
     CDS             complement(3084..3332)
                     /gene="hybG"
                     /locus_tag="ACHJ48_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO4707003.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHJ48_001415"
     CDS             complement(3345..3686)
                     /gene="hypA"
                     /locus_tag="ACHJ48_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO4707004.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEVHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHJ48_001416"
     CDS             complement(3679..4167)
                     /gene="hybE"
                     /locus_tag="ACHJ48_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO4707005.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHALSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHJ48_001417"
     CDS             complement(4160..4654)
                     /gene="hybD"
                     /locus_tag="ACHJ48_001417"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO4707006.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHJ48_001418"
     CDS             complement(4654..6357)
                     /gene="hybC"
                     /locus_tag="ACHJ48_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO4707007.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHJ48_001419"
     CDS             complement(6354..7532)
                     /gene="hybB"
                     /locus_tag="ACHJ48_001419"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO4707008.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHJ48_001420"
     CDS             complement(7522..8508)
                     /gene="hybA"
                     /locus_tag="ACHJ48_001420"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO4707009.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHJ48_001421"
     CDS             complement(8511..9629)
                     /gene="hybO"
                     /locus_tag="ACHJ48_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO4707010.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHJ48_001422"
     CDS             complement(9818..10105)
                     /gene="yghW"
                     /locus_tag="ACHJ48_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO4707011.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHJ48_001423"
     CDS             complement(10224..11111)
                     /gene="yghX"
                     /locus_tag="ACHJ48_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO4707012.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIFAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLNSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11268..12308
                     /gene="gpr"
                     /locus_tag="ACHJ48_001424"
     CDS             11268..12308
                     /gene="gpr"
                     /locus_tag="ACHJ48_001424"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO4707013.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLVGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHJ48_001425"
     CDS             complement(12348..12842)
                     /gene="yqhA"
                     /locus_tag="ACHJ48_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO4707014.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13033..13917
                     /gene="yghA"
                     /locus_tag="ACHJ48_001426"
     CDS             13033..13917
                     /gene="yghA"
                     /locus_tag="ACHJ48_001426"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4707015.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14189..14614)
                     /gene="exbD"
                     /locus_tag="ACHJ48_001427"
     CDS             complement(14189..14614)
                     /gene="exbD"
                     /locus_tag="ACHJ48_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO4707016.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14621..15355)
                     /gene="exbB"
                     /locus_tag="ACHJ48_001428"
     CDS             complement(14621..15355)
                     /gene="exbB"
                     /locus_tag="ACHJ48_001428"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO4707017.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15607..16794
                     /gene="metC"
                     /locus_tag="ACHJ48_001429"
     CDS             15607..16794
                     /gene="metC"
                     /locus_tag="ACHJ48_001429"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO4707018.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16934..17593
                     /gene="yghB"
                     /locus_tag="ACHJ48_001430"
     CDS             16934..17593
                     /gene="yghB"
                     /locus_tag="ACHJ48_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO4707019.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17633..18532)
                     /gene="yqhC"
                     /locus_tag="ACHJ48_001431"
     CDS             complement(17633..18532)
                     /gene="yqhC"
                     /locus_tag="ACHJ48_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO4707020.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMARPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18726..19889
                     /gene="yqhD"
                     /locus_tag="ACHJ48_001432"
     CDS             18726..19889
                     /gene="yqhD"
                     /locus_tag="ACHJ48_001432"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO4707021.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLGVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19994..20821
                     /gene="dkgA"
                     /locus_tag="ACHJ48_001433"
     CDS             19994..20821
                     /gene="dkgA"
                     /locus_tag="ACHJ48_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO4707022.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     FDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHNRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21021..21943
                     /gene="yqhG"
                     /locus_tag="ACHJ48_001434"
                     /pseudo
     CDS             21021..21943
                     /gene="yqhG"
                     /locus_tag="ACHJ48_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
     gene            21992..22249
                     /gene="yqhH"
                     /locus_tag="ACHJ48_001435"
     CDS             21992..22249
                     /gene="yqhH"
                     /locus_tag="ACHJ48_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO4707023.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22292..24511)
                     /gene="ygiQ"
                     /locus_tag="ACHJ48_001436"
     CDS             complement(22292..24511)
                     /gene="ygiQ"
                     /locus_tag="ACHJ48_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO4707024.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQCQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24622..26034)
                     /gene="ftsP"
                     /locus_tag="ACHJ48_001437"
     CDS             complement(24622..26034)
                     /gene="ftsP"
                     /locus_tag="ACHJ48_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO4707025.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26109..26846)
                     /gene="plsC"
                     /locus_tag="ACHJ48_001438"
                     /gene_synonym="parF"
     CDS             complement(26109..26846)
                     /gene="plsC"
                     /locus_tag="ACHJ48_001438"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO4707026.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27080..29338)
                     /gene="parC"
                     /locus_tag="ACHJ48_001439"
     CDS             complement(27080..29338)
                     /gene="parC"
                     /locus_tag="ACHJ48_001439"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO4707027.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29476..31083)
                     /gene="ygiS"
                     /locus_tag="ACHJ48_001440"
     CDS             complement(29476..31083)
                     /gene="ygiS"
                     /locus_tag="ACHJ48_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4707028.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGEIRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAMQITDATKRNALYQQAEVIVNQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31192..31674)
                     /gene="ygiV"
                     /locus_tag="ACHJ48_001441"
     CDS             complement(31192..31674)
                     /gene="ygiV"
                     /locus_tag="ACHJ48_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO4707029.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCRPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETNVYVPLA
                     "
     gene            complement(31727..32119)
                     /gene="ygiW"
                     /locus_tag="ACHJ48_001442"
     CDS             complement(31727..32119)
                     /gene="ygiW"
                     /locus_tag="ACHJ48_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO4707030.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32271..32930
                     /gene="qseB"
                     /locus_tag="ACHJ48_001443"
     CDS             32271..32930
                     /gene="qseB"
                     /locus_tag="ACHJ48_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO4707031.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32927..34276
                     /gene="qseC"
                     /locus_tag="ACHJ48_001444"
     CDS             32927..34276
                     /gene="qseC"
                     /locus_tag="ACHJ48_001444"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO4707032.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPVMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            34386..34967
                     /gene="mdaB"
                     /locus_tag="ACHJ48_001445"
     CDS             34386..34967
                     /gene="mdaB"
                     /locus_tag="ACHJ48_001445"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO4707033.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34998..35312
                     /gene="ygiN"
                     /locus_tag="ACHJ48_001446"
     CDS             34998..35312
                     /gene="ygiN"
                     /locus_tag="ACHJ48_001446"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO4707034.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(35357..36244)
                     /locus_tag="ACHJ48_001447"
     CDS             complement(35357..36244)
                     /locus_tag="ACHJ48_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO4707035.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLDQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36241..37188)
                     /locus_tag="ACHJ48_001448"
     CDS             complement(36241..37188)
                     /locus_tag="ACHJ48_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO4707036.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIERLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(37199..38236)
                     /locus_tag="ACHJ48_001449"
     CDS             complement(37199..38236)
                     /locus_tag="ACHJ48_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO4707037.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FFVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38245..39228)
                     /locus_tag="ACHJ48_001450"
     CDS             complement(38245..39228)
                     /locus_tag="ACHJ48_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO4707038.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39225..40034)
                     /locus_tag="ACHJ48_001451"
     CDS             complement(39225..40034)
                     /locus_tag="ACHJ48_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4707039.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARVLQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINTLTGKPFFMPFRGVEKV"
BASE COUNT         9998 a        10786 c        10066 g         9271 t
ORIGIN      
        1 ataatcagta cgggattgct acaacatgta ttactctttc accacaatta acggttcaat
       61 atcactctct tttttaataa ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgccaacg gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttacca ctggttttgt ccagcacctc
      241 ttcatgatgg ctgacgaggt cgatgttgct gccacagcga ccggcgatcg gtttcactgc
      301 gtaacctgtt ttcaccaatt catcattaac agtgaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca
      421 cagcggctca aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcatt
      481 ttgcggatga ccggtacgga tcggcaccgc agcaaactca cggtcgctaa cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tccagcccgc gcaagatacg
      661 cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc
      721 gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc
      781 agccagttcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agcccgcttc ggtatgacag gaggcggagt cggcgttgta
      901 ctcgtaaacc ttcagaccac gctcatccat gcagaaatcc atacgaccgg tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg gaatgtcgaa
     1021 cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg
     1081 cagctcgttg gtggctttaa tcagctcctg ctcggcgctc tcggtaatgg tgtagtaatg
     1141 atacggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gcagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccagacgcg ctccgctgat
     1261 tttcagcagc tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg
     1321 gatcatccag cccagaatgg tggtgtcatc aaaagtgtct ttaagggtat agcagccgtt
     1381 ttcgaccacc atctccagtt cgcgcgtcca ctgttgccct tgcggcaacg gggaatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgtaat tgggtaatga tggcgacatg
     1501 gccagtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc
     1561 acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttcacg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta
     1681 gttcagaaag agaaaacggc gagcaaattc aacgcattgc catttgtggc ccatatattc
     1741 gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc ggtgggcaaa
     2221 catccattac aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctgc tcgcgctggg cgttactggc gcagagtacg atgcctggct gatacgcata
     2341 ggcgatggtg atcaattctc cagcggcttt gtcgaagtga acccaaattc gaagatcccg
     2401 gcgctgcgcg atcataccca taatccgccg atccgcgtgt ttgagtcagg atcgattctg
     2461 ctttatctgg cggagaaatt tggctacttc ctgccgcagg atctggcaaa acggactgaa
     2521 acgatgaact ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcgcc ggtgaaaatt gagtacgcca tcaatcgctt taccatggaa
     2641 gccaagcgcc tgctcgacgt gctggataag caactggcgc agcataagtt tgttgcgggc
     2701 gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaacgt ggtgctcggc
     2761 ggcgtgtatg atgccgcaga gtttctggat gcgggaagtt ataaacatgt gcaacgctgg
     2821 gcgaaagaag tgggcgaacg tccggcagtg aagcgtgggc gtattgttaa ccgcaccaac
     2881 gggccgctga acgagcagtt gcatgagcgc catgacgcca gtgattttga gacgaatacg
     2941 gaagataagc gtcaggggta aggattggtg ttcgtcgcag caagccatcc aggccggata
     3001 aggcgttcac gccgcatccg gcaatcgagc acaatgcctg atgcgacgct gccgcgtctt
     3061 atcagaccta cgaaagctaa tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt
     3121 gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt
     3181 accagcaccc actggcccag cagatcggca gggttacctt cacaaatcag ggcgatattc
     3241 acgtcgcgct tgataccaca tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca
     3301 gccagcacct ggcctggaac gccaatacac attgttaact ccggttattc aacttcaata
     3361 cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg acagagcgga
     3421 cactgcgcat cgtgctgatg aacctccacc acctggctgc aatcccagca ccaggcctgg
     3481 gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt
     3541 tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat ttccagccac
     3601 acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gctggataat ttcaacggcg
     3661 ctctggcaaa gcgacaactc atgcattttc gccactcctg cgaccaaaca gcagggcgcg
     3721 acgccctgcg tgtggtacat ccggattcgt gactggcagc gaaaggatca ttcgtgcgca
     3781 gtcatccgtc aggcgttggc cctcttcaat cgacatactg tgcgaaagcg gtgacatcag
     3841 cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg tcatggtgcc
     3901 atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg
     3961 gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc
     4021 ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagagc
     4081 gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt
     4141 ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct tccacgccag
     4201 attcacgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct tcaaccgtcg
     4261 gcgttaagcc gatgtgtggc tccagcgatt ccggaattac gccgaccagg gtcagctttt
     4321 tcggaaactc gccggtgaag cgcagggccg acaagacgtc ggccaggcca agctgatgcg
     4381 gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc
     4441 ccggcgcgct cttcttcgac acaatggcat ccgcaataat cagatgatcg cgatttgcca
     4501 tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca
     4561 gaatgtatcg ttgctctaac gcttcgacaa tccgcacacc gatggcttca tcggtcagca
     4621 aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc
     4681 gttgccgtca gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat
     4741 ggtacgcacc acttccagcg gtttattcgg atcagcaacc ggtgtaccca ccagcgactg
     4801 ctcgtaagga ccgacgtcat cattgaagtt acgcgggcca gagttccagg ttgatggaac
     4861 aactgcctgg tagttgctga tgataccgtc tttgatcacc atccagtgag agagcatacc
     4921 gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa tgttcggctt
     4981 cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attggttttg
     5041 caggatatcc tgcaattcac agcagtgaac ggtacgacca ataatacggc ccagcgtgga
     5101 gtgcagctgt gccacttcca gcgtgttgcc agtcagtttc tgataaatcg caacgatttc
     5161 attcagtttg ttctgggtag attcgcgacc tgccgccagt ttcaccagca tattagccag
     5221 cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttccc
     5281 gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggtg cctgcggcgc
     5341 ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca ggtattcatc
     5401 ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt agccgcccgg
     5461 gaacaggaag ctgccgtttt tactgtcggt cgggaattcc ggcacgctca ggtagttcac
     5521 cgcaccttta ccgcgcgtca gccattccgg gtagaacgca gcgataaccg cagtatcgac
     5581 cttgtaaacc tgctcaacaa agtcactcag cttgtcgatg aaagacttga tgtacatcag
     5641 gcgctcaagg ttcagcacgc ccagaccgtc gaggttgatt gggttagcga ccccacctac
     5701 cgccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc
     5761 acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg
     5821 cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact gaccgctggc
     5881 aaccagatct ttgatcttgt tctgaacttt ggtgaactct tccggactgt tcaggtgcca
     5941 ggtcgaaaca cctttcagca tttcggaggc tttggttggg tcagcttgca gtgcagaagt
     6001 gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt
     6061 gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag
     6121 cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat
     6181 acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc
     6241 gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc
     6301 gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct
     6361 ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga
     6421 cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt
     6481 tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac cagcgaatag
     6541 gtcagacgcc aggttgcaca acctaacagc gcgctcagtg ctgacaggaa cagcatgcgg
     6601 gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact
     6661 tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga
     6721 tagataagct cgccaaagcg cagcacgatg aaaatcgcca gcaacacact gatggtgttg
     6781 gtcagcttga cgaataggct cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc
     6841 agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac
     6901 agcggcaaca tttcatagct ctgccacagc ggatgcacct tgtagcccgc cgagatcatc
     6961 agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc gatgatgaag
     7021 aacatcacct tgttcagacg ctgtagcgac accttccagc ccagacgttc aaacagtgcc
     7081 ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac
     7141 agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga
     7201 cccacgtcga tagtgatcga caagccaccc agtgagtaac caaacagact cgccaacagc
     7261 gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgcaagc
     7321 gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc
     7381 cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca
     7441 atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt
     7501 ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt
     7561 tttggtgttg cgacgaacca gcacggtcaa gcccgccagc acagccagtg gtagcatcat
     7621 gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag
     7681 tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc
     7741 gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc
     7801 gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg
     7861 ttttttcgcc tccgccatca gctcttcacg cgtaccaaaa atcaccgcgc ccgccgggca
     7921 cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca
     7981 cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta
     8041 cggacaggcg accatgcagt aacggcaacc ggtgcacacg tctttgtcgt aatggacaat
     8101 gccggttttc ggatcttttt tcagtgcaga gaccgggcac acagagacac agttcggatc
     8161 gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt
     8221 gacccctgtg ccgctggtcc acacttgaat gatgttattg gtatacggcg acagtttgtc
     8281 gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga tatcctgaca
     8341 cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga
     8401 tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt
     8461 cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc
     8521 cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg
     8581 acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc agagacgttg
     8641 ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtctgatt ttcgacgttg
     8701 gcaagctgat ggatgccttt atggaagccg ataccttctt cgttacagcc atagcacggg
     8761 tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt tgagcagttg
     8821 ccgtaagttt ctggcccttt acagccgagg tggtacaggc accagccttc gcggtggcct
     8881 tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt
     8941 tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt
     9001 ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag
     9061 cccggaatat tgataacggt tttgcctggc agaacttctt gcaagctgac tgcgccagtt
     9121 gggttaactc cagctgcggc aacaccgccc cacgcagagc aggaaccgat agcgataatg
     9181 gctgctgcgc cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg
     9241 caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct
     9301 ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa
     9361 agcacttcgt gatactccag agagatagtc tccagtacga ggttttctac cgttggatgc
     9421 gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact
     9481 ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac
     9541 cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga
     9601 gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgaa gacctggcat
     9661 atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta
     9721 ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta
     9781 gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg
     9841 ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct
     9901 ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat
     9961 ctccggtctt ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg
    10021 cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta
    10081 agcctttacc aaaatggtta ttcatcgttc acctcattcg ctatatatcg taatatatag
    10141 cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatgctt gtttaagacg
    10201 taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt
    10261 tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg
    10321 tgattaaccc ccggatagat atacgcctca taaaccttat tattggcttt caacgccgcc
    10381 tcgtaagcag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa
    10441 ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcactgcg
    10501 caggccagtt ccgggtaagc gaccgctgcc gcgttcgata cgccaccgcc atagcaaaat
    10561 ccggtaatac ccactttgcc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca
    10621 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    10681 tcatttcccg gataacctcc aacggaattt aagccgtcag gtgccagggc gatatacccc
    10741 gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc
    10801 accaccacca cggctggcgt tttgccgctc atttttgcgg gtttaaccag ataaccccgt
    10861 acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaaaaat ctccgggtcg
    10921 gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat
    10981 gccgtcatcc cgcctaccgc atacttcgcc gcaaggttga ggaactcacg tttcgagatt
    11041 ttcccgtgag cgtaatagtc gtagtaatcc agcaactctt gtgggaaatc tttggcggtt
    11101 aaacgcggca tcgttgcact cctcagttgg tgttttttta agcaaagcat aagcacgtgt
    11161 ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc
    11221 taaactagaa acattgtttc gaaattgaac ggtggaaagg agaggtcatg gtctggttag
    11281 cgaatcccga acgttacggg cagatgcaat accgctattg cggaaaaagc ggtttacgcc
    11341 tgcccgcgtt atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac
    11401 agcgtgcgat cctgcgtaaa gcgtttgatt taggcattac gcactttgat ttagccaaca
    11461 attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg cgggaggatt
    11521 ttgccgctta tcgcgatgaa ctgattatct ctaccaaagc tggctacgat atgtggcccg
    11581 gcccttacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac caaagcctga
    11641 agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc
    11701 cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg
    11761 tcgggatctc ctcttactca ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt
    11821 ggaaaattcc gctgttaatt catcaacctt cgtacaattt actaaaccgc tgggtggata
    11881 aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgcattgcc tttactcctc
    11941 tggctcaggg attgctgacc ggaaaatatc tcaacggcat tcctgaagat tcacggatgc
    12001 atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gctcaccgaa gccaacctca
    12061 acagcctacg cttattgaat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg
    12121 cgttaagctg gttgctgaaa gatgagcgag tgacgtcggt attggttggt gccagccgcg
    12181 cggagcaact tgaggagaac gtgcaggcgc tgaataatct gacatttagc accgaggagc
    12241 tggcgcagat cgatcagcat atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg
    12301 ataaatgacc tgttaataac gggcgcgaga accgcgcccg aatcagatca gtgattatga
    12361 cgagtcagtc ggtcaagata gcccattaca aatgcagaga gcacaaacgt cagatggata
    12421 atgacgtacc acatcagttt gttatcaggg acatttttcg catccataaa gacgcgcagt
    12481 aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgca
    12541 tccattttcc ccagccagtt cagcttctct ttgttctcgg agatatcgag ttgtgagacg
    12601 aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc
    12661 accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt
    12721 acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca
    12781 aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc
    12841 atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag taaacagcgc
    12901 gcaacagcaa atcaacatat ccgttaaatc tttgtcgcat aattctgagg gttagggatc
    12961 ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa
    13021 gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac tggtgaatat
    13081 cccaaacaga aacaaccgac gccaggcatc caggcgaaga tgacaccggt accggattgc
    13141 ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagccct ggtgacaggg
    13201 ggcgattccg gaatcggtcg cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg
    13261 gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa
    13321 gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa atttgcccgt
    13381 tcgctggttc acgaagcgca taaggcctta ggtgggctgg atattatggc gctggtcgcc
    13441 gggaaacagg ttgccattcc tgatattgca gacctcacca gcgaacagtt tcaaaagacc
    13501 tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg
    13561 aaaggtgcaa gtattatcac cacttcgtca atccaggcat accagccaag cccgcattta
    13621 ctggactatg cagctacgaa ggcggcgatt ctgaactaca gccgaggcct ggcaaaacag
    13681 gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gcccgatctg gacagcactg
    13741 caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagca aacgccgatg
    13801 aaacgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag
    13861 tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa
    13921 aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc
    13981 gaaaacgcct tatccgccta caaatgcatg aagattcaac atgttgcaat agattttgta
    14041 ggcctgataa gcgtagcgca tcaggcattg agcacctaat gccggatgcg gcgtgaacgc
    14101 cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga
    14161 tgaacgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca
    14221 gacctatctt caggtaaccc gcctgatgca gcgtgtccat taccttcatc aacgtctcgt
    14281 aatcgacagt tttatccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg
    14341 cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt
    14401 tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag
    14461 aagcaggcaa gttaaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa
    14521 tcagcaggac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt
    14581 tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt
    14641 gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca
    14701 gcaacaatac ctgcgctgcg acatcaccca gcatcgcttt aaagccgcca atctgacgcg
    14761 cgaatacgtt atagataacg accgccggaa tcgctgccac cagaccgatt gccgttgcta
    14821 acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga
    14881 taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagcccaacg aacggcgaaa
    14941 tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgacct actgcggcga
    15001 cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt
    15061 ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac
    15121 caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg cgcttcagcc agcagttgct
    15181 gctcgcgctt aaggcgacgc ttctgattaa agaactctac gctcttactg aagaagattg
    15241 cccaggtgac tacggaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg
    15301 cgtgctgata cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt
    15361 atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga
    15421 tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta
    15481 cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt
    15541 caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat
    15601 cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa
    15661 tacactctcg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt atttgagagt
    15721 atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg
    15781 cgcggaacgt taacccattt ttccttacaa caagcgatgt gtgaactgga aggtggcgca
    15841 ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct tgcttttgtc
    15901 gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt
    15961 agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc
    16021 gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccgggttcc
    16081 atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg
    16141 gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat
    16201 tttggcatcg atgtttctat tcaagccgcc accaaatatc tggttgggca ttcagatgcg
    16261 atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaatgcctat
    16321 ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca
    16381 ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggccga atggctggca
    16441 gaacatccgc aagttgcgcg agttaaccac cctgccctgc ctggcagtaa aggacacgaa
    16501 ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa
    16561 ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag catggcctac
    16621 tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgcagccatt
    16681 cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa
    16741 gatgtcgacg atctgattgc cgatctggac gccggttttg cgcggattgt ataacattgc
    16801 cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg
    16861 atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta aacgctgttc
    16921 cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt
    16981 tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct
    17041 gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatatt
    17101 ggctggcgca ttgattgccc agggggttat ggattttctg cctacgattg cgattctgac
    17161 cgccgcagca agtctgggct gctggctaag ttatattcag gggcgctggt tagggaatac
    17221 caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc gcgccacctg
    17281 catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg
    17341 tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgtt tccagttttt
    17401 caactggtta agcggattgc tgtgggtcag cgtggtaacc agtttcggct atgccttaag
    17461 tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc
    17521 aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata
    17581 ctgtaacgcc tgacgatttt ccctgttccc ggttgctgta ccgggaacgt atttaattcc
    17641 cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa
    17701 actcacggct aaattgcgat gcgctttcat agccgacgcg catcgctgct gcgctggcct
    17761 tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact
    17821 gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatat
    17881 tagcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaatccgtt
    17941 tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc
    18001 cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa
    18061 tacgcgcatc cagtggtcgc gccatcacgt cgagtaaccg ctccgccgcg cataaaatct
    18121 cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat
    18181 cttcgccaat atccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc
    18241 ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca
    18301 gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat
    18361 gtccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg
    18421 tctcgccata caacaaacgc acatcgggca acagttctga caaactattt tctttatttt
    18481 tcagtttatt aactttatcc gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta
    18541 atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac
    18601 tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat
    18661 cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc
    18721 aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa
    18781 tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggtggcg
    18841 gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg
    18901 tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga
    18961 aactggttcg cgaacagaaa gtgactttcc tgctgggggt tggcggcggt tctgtactgg
    19021 acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca
    19081 ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc
    19141 tgccagcaac cggttcagaa tccaacgccg gtgcggtgat ctcccgtaaa accacaggcg
    19201 ataagcaggc gttccattca gcccatgttc agccggtatt tgccgtgctc gatccggttt
    19261 atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca
    19321 ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag
    19381 gcattttgct gacgctgatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg
    19441 atgtgcgcgc caacgtcatg tgggcggcga cgcaggcgct gaacggtttg attggcgctg
    19501 gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc
    19561 tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata
    19621 ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact gaaggttccg
    19681 acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg
    19741 tgcctaccca cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa
    19801 aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg ctggatgtca
    19861 gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg
    19921 catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat
    19981 aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc
    20041 ccagttggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa
    20101 agcgttagaa gtgggttatc gctcgtttga taccgccgcg gcctataaga atgaagaagg
    20161 tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa
    20221 gctgtggaac gacgaccaca atcgcccccg cgaagccctg ctcgacagcc tgaaaaaact
    20281 ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta
    20341 tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg
    20401 cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc
    20461 tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcctggaa
    20521 cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt
    20581 tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc cggcgcagat
    20641 tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc
    20701 acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcga
    20761 aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta
    20821 acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag aaatcattta
    20881 ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac
    20941 atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc
    21001 aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt
    21061 ttaccgtgca cgcacagccc ccctcacaga ccgtagaaca aacagtccgg catatttatc
    21121 agaactataa atcagatgcc actgcccctt attttggtga aaccggagag cgtgcgataa
    21181 cttctgtgcg tattcaacag gcgcttaccc tgaacgacaa tcttacgctg ccgggtaata
    21241 ttggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgctagaaag
    21301 cgtagcgata accgaaactg acgccgatca tgccgatgcc gttgtgcgct ttcgtatctt
    21361 taaagatgat aaagaaaaga ccacgcagac actgaaaatg gtggcggaaa atggtcgttg
    21421 ggtcattgac gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    21481 tgaaaaaacg ctggccgctt tagcttcgtt gcaaaaagaa cagccggaag cctttgttgc
    21541 cgaactcttt gaacatattg ccgattatag ctggccgtgg acgtgggtgg tttccgactc
    21601 ttaccgccag gcggtcaatg ccttctataa aaccaccttc aagacggcca acaatcccga
    21661 tgaagatatg caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    21721 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    21781 cattcatatt cgttttacgc tgaccaatgg caacaacgaa gagcaagaac tggttttaca
    21841 acggcgcgaa ggtaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact
    21901 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaatagcaa
    21961 ttagccggaa caataaaaaa gggaacacta tatgaaaacg attttcaccg tgggagctgt
    22021 tgttctggca acctgcgtgc tcagtggctg cgtcaatgag caaaaggtca atcagctggc
    22081 gagcaatgtg caaacattaa atgccaaaat cgcccggctt gagcaggata tgaaagcact
    22141 acgcccacaa atctatgctg ccaaatccga agctaacaga gccaatacgc gtcttgatgc
    22201 acaggactat tttgattgcc tacgctgctt gcgtatgtac gcagaatgat aaaaaaatcc
    22261 ccggcagcat gtcagttgcc ggggattttt tttaacgtcc aaccgccgct ttagggcgtt
    22321 tcttcgcacc agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag
    22381 gcgtctgaca ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt
    22441 tctggcgacg agcctcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac
    22501 ggctgccaat cagatgcttt ttgcccatcg cttccagcgc ctggcggatt aacggccagt
    22561 ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg acgctgtttg tcgcccttcg
    22621 gtacgaagac gtcttcactc ttataaccaa tcttcgccag cgggtttttc ccggtgtaat
    22681 acatggtggt cgagttagcc agtggcgatg ggtagaagtt ctgtacctgg tcgagacgga
    22741 aacgatgctt tttcagccac agcgccaaat tcaccatatc ttcatcacgc gtaccggggt
    22801 gcgcggagat gaaatagggg atcagatact gctctttacc tgcctgtttc gagtaggtat
    22861 cgaacagctc tttaaagcgg tcataactgc ccatgcccgg cttcatcatc ttcgacaacg
    22921 gcccttcttc ggtatgttcc ggagcaatct tcagataacc gccgacgtga tgggtcgcca
    22981 gttctttgat atagcgcgga tcttccacgg cgatgtcata acgtacgcct gaggcaatca
    23041 gaatcttttt aatgcctttc agatcgcgag cgcggcgata gaggttgatc gtcggttcgt
    23101 ggttagtgtc catatgcgga caaatatccg ggtaaacgca ggacaaacga cggcaggttt
    23161 gttcagcgcg tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat
    23221 cggaaattac gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga
    23281 tcgaatcttc ggaacggctc tgaataatgc gcccttcgtg ctcggtgata gaacagaaag
    23341 agcagccgcc aaagcagccg cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg
    23401 gaatacgggc attgccatag gccggatgtg gcacgcgctt gtacggcagc gcaaaaacgc
    23461 tgtccatctc ttcggtagaa agcgggatag caggcgggtt gatccacaca tagcggtcac
    23521 cgtgtttttg catcaatgcg cgggcgcaac ctgggttagt ttcgtggtgc agaatacgcg
    23581 aagcatgggc gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt
    23641 aggttttttc ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct
    23701 ttttcggtgc caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga
    23761 ttgggtcgat ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca
    23821 gcgcctcttt cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc
    23881 ccatcgccag acggtgcgct acctccacca gcggacgctc accgttacca aacatcagca
    23941 tgtcggcttt cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat
    24001 gcgcggtacg gcgcagacta gcctcaatac caccgaggat caccggtaca tctttccacg
    24061 cctctttaca acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt
    24121 tatccggcgt gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca
    24181 tcgaatccat gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcatga
    24241 aatcgtcttt gctgctccag tctggctggg cgatgatccc gacgcgaaag ccctgtgctt
    24301 ccagcatacg accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag
    24361 taaccaaaat gatgtcgcag ctatcccagc caagttgatc catctcttca cgagacatcg
    24421 gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc
    24481 gatccggttg gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat
    24541 caaaggccgg ggattataag ccggaacgaa agagaaatcg aaaagtattc catactcgcc
    24601 ctcctcgggc gagtatgaag attacggtac cggattgact aacagttgcc caatcgagcc
    24661 acggtccgcc atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg
    24721 ctgaccgaaa taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc
    24781 tctgtcttcc gggaacggca tcgcaccatt cacgttacgg atctggaaca tcacgccttc
    24841 aatatggaac gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg
    24901 cgcggtgaca tcaatacggt tgacgtccca cagttgtcca ttaatacccg ggtcatcacc
    24961 cagactgata tcgcgactgc gaatcggcga accggccatg atttcagttg gcagcaagcg
    25021 catcggaaga ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag
    25081 ggtagaaacc agaatgctgg atggctcaaa gaagccacga atacgatcaa caatgctcgc
    25141 cgcttcgcca caggtgatcg acacttcatc accgttgctc atatccacca gaatctcgcg
    25201 gcgctcgccc ggtgccaacg aaagttgctt caccgataca ggagcaggca ggaatccctg
    25261 atcgccagaa atcacatgta acgggcgacc atcgctcatc tgtagttgat agcgacgaga
    25321 gttcgacgcg ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct
    25381 ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta
    25441 ttctggcgta ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac
    25501 accataatgg ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc
    25561 ggcaaggccg ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag
    25621 agtagctgcg ttctggcgaa taggcagtac gggtgcccag tcagcgtttg gcgacatcat
    25681 ccgtgccgga ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac
    25741 attttctgtc aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat
    25801 agtcggcccc aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa
    25861 tgaccagtgc gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagcag
    25921 cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc
    25981 tgcacaaagt gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc
    26041 tccatgtaaa actggctaaa gtaataccgt gcaggaaagt gtttcccgca aaaatacaga
    26101 ttgccgactt aaacttttcc ggcggcttcg cgctctgcga cttctttatc gagctcggcg
    26161 attttttgtt ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca
    26221 tactgactga cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga
    26281 ttaagattaa tcttattcga agttgtggag acgcacacgg gaataatcgg gacgcccgcc
    26341 gcaattgccg cgtgaaatgc cccagtcttg aacggtagca ggccgcgacc acggctgcgg
    26401 gtaccttccg ggaacatcca gatggaaata cggcgttttt tgaaatgatt cactacttcc
    26461 gcaatagtgc cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac
    26521 cagtacaact gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc
    26581 ggcggttgca caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata
    26641 tagatagcat tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg
    26701 aacagcggcg caagacgacc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc
    26761 gggctgaaaa ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta
    26821 ataataagac gaaagatata tagcataaca ccctctgaag gtctgagaga ctggcattgt
    26881 acgtcacctg aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt
    26941 taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg
    27001 aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa
    27061 cagcgccctc cccggcatat tactcttcgc tatcaccgct gctggcacgg cgaggagagt
    27121 cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt
    27181 cgccagtgac tttctgtagc tcttccggac gcagtttaat tttgcgtttc ccaacatgaa
    27241 tggtcagcgt gctttgcggc ggcagaacgt acagttgcgc cagaccatcc tcgccacgcg
    27301 cggcttctgc cgatggaatg ttgataatct tgttgccttt gcccttcgac agctgcggca
    27361 gatcacttac cgggaacatc aacatacggc ctgcctgagt gattgccagc agcatatcgg
    27421 aagcatcttc aattaccacc ggcggcataa catgggcatt ttccggtaag gtgatcaaag
    27481 ccttacctgc acggttacgc gccaccagat cgttaaaggt gcagacgaaa ccgtaacccg
    27541 catcggaagc catcagcagt ttctgatcgt cgctttccat cagcatatgg tcaacggtcg
    27601 ccccaggcgg caacgttaat ttgccggtga gcggttcgcc ctgaccacgc gccgacggca
    27661 gcgtaatcgg atcaatggca tagctacgac cggtggaatc aacaaacact accggttggt
    27721 tgctcttacc tttcaccgcc gctttgaagc tatcacccgc tttataattc aggcccggcg
    27781 cgtcgatatc atggccttta gcgctgcgta cccagcccat ctgcgacagc acaatggtga
    27841 caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg cgttcctgca
    27901 acggcgaacg acgatcgtca ccgtaggctt gcgcgtctgc ctgcagttct ttcttcagca
    27961 ggttattcat tttacgctcg gaagccaaaa tgccctgcaa ctggtcgcgc tctttttcca
    28021 gctcactctg ctcaccgcga atcttcatct cttccagttt ggcaagatga cgcagtttca
    28081 gttcgaggat cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc agcgccggtt
    28141 tcggttcatc ttcattacga atgatctcaa tcacttcgtc gatattgaga aacgccacca
    28201 gcaaaccttc gaggatatgc aggcgcttga ggactttctc cagacgatag ttcagtcggc
    28261 ggcgcacggt atcgcggcgg aacaccagcc attcggagag aatttccagc aggtttttca
    28321 ccgccggacg accatccaga ccgatcatat taaggttaat gcgatagctc ttttccagat
    28381 cggtggtagc gaagaggtgg ttcatcacct gatccatatc cacgcggttg gaacgcggca
    28441 caatcaccag acgggtcggg ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg
    28501 gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca cctgaaacct
    28561 gatgcggcaa tgcgctgata accaccgcgc catcttcttt cttccacacc gcgcgcatac
    28621 gcactgaacc acgtccgttc tcgtagattt tacggatctc ggcgcgcgaa gtgataattt
    28681 ccgcttcagt cggataatcc ggcccctgca cgatatccag cagctgatcg agcgtggttt
    28741 tcggctggtc gattaatgcg attgccgcct gagccacttc acgcaggtta tgcggtggaa
    28801 tatcggtcgc catgccgacg gcaataccgg tggtgccgtt aagcaaaatg tttggcagac
    28861 gggcaggtag cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc cagtcagccg
    28921 tcccctgccc caactcgctc aatagcagct cggaatattt cgacaaccgg gattcggtgt
    28981 aacgcattgc cgcgaacgat ttcggatcgt ccggcgcgcc ccagttcccc tgaccatcaa
    29041 ccagcggata acggtaagag aacggctgcg ccatcaggac catcgcttca taacaggcgc
    29101 tatcgccgtg cggatggtat ttacccagta cgtcaccgac ggtacgggcc gattttttaa
    29161 atttggcgct ggcattcagg cccagttcag acatcgcata cacaatgcgg cgctgaacag
    29221 gtttcagacc atcaccaata aacggcaacg cacggtccat gatcacgtac atggagtagt
    29281 ttaagtaggc gttttccgta aattcatgta gcgcaaggcg ctctgccata tcgctcatta
    29341 attctgattc ctcaacttat tcgccagcct tcaaaggggc aatattgccg gagatactac
    29401 attatctgcc gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg acgtgctccg
    29461 acggcgataa aggcatcaat gtgccttgat atacaactct ttgctgtaga cataatcctg
    29521 gggattatgc agcggaaaac cgccaacgta gggtttaagc agtttgatta acggctgata
    29581 gtagatagga atcagcggtg cctgctggtt gacgatcact tctgcctgct gatacaacgc
    29641 attacgcttt gtcgcatcag tgatctgcat ggcctggttt agtaaggcgt catactgcgc
    29701 atttttccag tgaccgacgt tttcttcact atcgcttttg agcgtgttca ggaagctgga
    29761 agcatcattg tacgtcgcat cccacgactg ccgagacagc atgaaatcac cggctcgtcg
    29821 ggcatcaaga taggttttcc actccattgt gcgcagcgtc acctgtgcac ccagccattt
    29881 tttccattcg gaagacaacg ctatcgcggt cttttcatgc agatcgtact tgttgtagaa
    29941 cagctcaaag cgtagcggat gagaggcgtc gtatcccgcc tgtttcagca aggcttttgc
    30001 catcgcgacg cgctcactca ttggcttttg cagttcatcg aacgtcgtcg cgctaaagcc
    30061 ttttacctct ggcggcgtca gcgtggttgc gggcgttctc aaccccagta ccttttgcgc
    30121 aataagctgt cgatcaaccg taagatatag cgcccgacgc actcgcacat cgttaaatgg
    30181 cggtttctca aggttgaagt tgtaatattc gctgttcaga cgcggaataa ttcgtatctc
    30241 gccaggcagt gatttttcaa tggcgggaat ttgctgcgcc ggaacccagg tgagatcgac
    30301 ctctcccgcg cgatagcggt tatagccggt gaccgaatta tccagcgcca gatactcaac
    30361 ctgttgcaat actgtatgtt gcgcatcgcg gtactttgga tttttgcgtg cagtaatctt
    30421 ttcgttaact acccactgat caagcacaaa ggcaccgttg taaaccatgt tctctggctt
    30481 actccagcta tcgccatgtt tagcgatgac atgatgagga accgggaaca gcgtcggcca
    30541 ggcgagcatc gtcgtgaacc aaggaaccgg ctgctcaagc gtaacttcaa gagtacgatc
    30601 atccgtcgct ttgacaccca gcgatgtaac atctgcttta cccgcaacaa tagccgcggc
    30661 attgttaatg tgcgcctgtg ccagatagcc agcaaaaggg cttgccgttt tcgggtcaac
    30721 cgcgcgctgc cagccgagga caaaatcctc tgccgtcaga ggctgaccgt cggaccactg
    30781 caaaccgcta cgcagatgga aaatatagcg cctgccgccg tccagtatct cccagcgttc
    30841 agcctgagcg ggctgcacct ggccttcacc gtccatccat accagacctt caaacagatc
    30901 cagcacaatc tgcgcggcag tattctcctc caccttttgc gggtcgagcg tacctgggtc
    30961 gctatgattg ttgtaacgaa agacttgttg cggggcgagc tgtgtgttgg tgggaacgtc
    31021 agcagcgtag agaggagccg cacttaccag gctgaccagc cataataaat ttcgcgtata
    31081 catggtattc cttaccggag aaattataag cccaacaaca atacgcctgt ggcattgttc
    31141 gctcaaactt atcgcgagtg atttggctca cactccggta aagattcccc atcacgctaa
    31201 cggcacataa acattcgttt ccagtcgctg ctccgtcacc ccttcggcaa gattggtgta
    31261 gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt gcaggcagcc agtggcgaat
    31321 aatgccccat atcgtatgtg aaatatcgtc cagctcgcca acgtggcgag ccacggcata
    31381 acgtccaccg gtaagttcac cattgctcac accataacga ttatcgggaa tcggttcgct
    31441 aacgctgccg cagatatcaa agcgaaacgc ttccggtgcg gtggtggcag ggtcgtccca
    31501 ggcgacgcca aaagtctggc tttggttaac aggagaaagc cccgtctctt tacgccacat
    31561 gataaatttc gccacgctgt agttgagcaa ctcaggccta cagcggtgcg gcaacatcgc
    31621 cacagggatt gatgggaaat cgataatgtt tacatccagt gtcaggtttg tcatgttgcc
    31681 tccctgcgaa attattcccg ggaacgcatc ccgggagcgg taacaattac ggatttactt
    31741 tgcggatctg tttgacgtca atttcaacag aattccagtc tttatcgact tcaccctgaa
    31801 tctcaaccgt atctttcggc gtcaccgtca cgccgttcca gcgtttgtgg tcgatatcaa
    31861 cattgatagt accgctggca tctttgaaca cgtagagatc gtcagagatg cgttcaacga
    31921 tattgccgcg cagggttacc caggtgtcgt cacgcaggga ttttgcgctt tctacagtcg
    31981 ttacgctgcc gttcggcccc tggaatcctc cggcctgact ttgcgttgcc gatgggccag
    32041 aaaaaccgcc ctgctctgct gccatcaccg gtgcgctgca cagggccatt actgcgatta
    32101 ctgctgcgaa ttttttcatg tttattactc cctttaatgt ctgtttccga gcatttaaca
    32161 agatagtcct taacaacttc ttaagggaaa aaaataaaat ttagtgctgt acagagcgcg
    32221 ttacaacacg gtttactagc agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt
    32281 tactgataga agatgacatg ctgattggcg acggcatcaa aacgggcctt agtaaaatgg
    32341 gttttagcgt cgactggttt acacaaggtc gtcagggaaa agaggcgctt tatagcgcac
    32401 cttatgatgc ggtgatcctg gatttaacct taccaggcat ggatggtcgc gatattttgc
    32461 gcgaatggcg agaaaaaggt cagcgtgagc cggtactgat cctgaccgcg cgcgatgcgc
    32521 tggaggaacg tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg
    32581 cgttgataga agtcgccgcc aggctggaag ctctgatgcg ccgaaccaac ggccaggcca
    32641 gcaacgagct gcgccacggt aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg
    32701 ctggcgaacc cttaacactg aaaccaaaag aatttgccct gctggaatta ctgatgcgta
    32761 acgctggtcg ggtactgccg cgcaaactga ttgaagagaa actgtatacc tgggacgaag
    32821 aggtcaccag taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg
    32881 attttattcg taccgtgcat ggtattggct acacattagg tgagaaatga aatttaccca
    32941 acgtcttagt ctgcgtgtca ggctgacgct aatcttttta attctggcct cggtgacctg
    33001 gctgctttcc agctttgtcg cctggaaaca aacaacggat aacgtcgatg aattgttcga
    33061 cacccaactg atgctgtttg ccaagcggtt aagtacgctc gatctcaacg aaatcaacgc
    33121 ggcggatcgc atggcacaga cgccaaataa attaaaacac ggtcatgttg atgacgatgc
    33181 gctgaccttt gccatcttta cccacgacgg cagaatggtc cttaacgatg gcgataacgg
    33241 agaagatatt ccctatagct atcaacggga aggttttgct gacgggcaac tggtgggcga
    33301 taaagatcaa tggcgttttg tctggatgac ctcacctgat ggcaaatatc gcatcgttgt
    33361 tggtcaggaa tgggaatacc gtgaagacat ggcgctggcg attgttgccg ggcaattgat
    33421 cccgtggttg gtcgcactgc cggttatgtt aatcatcatg atggtactac tgggtcgtga
    33481 actcgcgccg ctgaacaaac tggcgctggc actacgtatg cgtgaccctg actcggaaaa
    33541 accactaaac gcgactggcg tacccagcga agtgcgacca ctggttgagt cgctaaatca
    33601 actgttcgcc cgcacacatg cgatgatggt tcgtgaacga cgctttacct ccgacgcagc
    33661 tcacgaactt cgtagcccgt taacggcgct gaaagtgcaa accgaagttg cgcagctctc
    33721 tgacgatgat ccgcaggcgc ggaaaaaagc actgctccaa ttacattccg ggatcgatcg
    33781 cgctactcgt ctggttgatc aactgctcac gctatcgcgg ctggactcac tggataacct
    33841 tcaggacgtc gcggagatcc cgcttgaaga tctcctgcaa tcgtcggtga tggatattta
    33901 ccacacggcg cagcaggcga aaattgacgt gcgactgaca ctcaatgtcc agggcatcaa
    33961 acgcaccgga caaccgctat tgctaagttt gttggtgcga aatttgctgg ataacgccgt
    34021 gcgttacagt ccacagggca gcgtggtgga cgtcacgctg aatgccgata atttcatcgt
    34081 aagggataac ggccccggtg tgacaccaga ggcactggcg cgaattggcg aacgcttcta
    34141 tcgcccaccc ggacaaaccg ctaccggcag tggacttggg ctatcgattg tccagcgaat
    34201 cgccaagctg catggcatga atgttgagtt tgggaatgcg gaacaaggtg gatttgaggc
    34261 gaaggtaagc tggtaagggt aagacttttg ctaaattcgc aaaagacttt gcacattttg
    34321 ctaatttcac cgtaccgctc tgtgacgtac tatagtcggc aaacgtctca ccttgaggtt
    34381 aaaaaatgag caacatcctg attatcaacg gcgcgaaaaa attcgcccac tccaatggtc
    34441 aactgaacga caccctgacc gaagtcgcgg atggcacact gcgcgacctc gggcatgatg
    34501 ttcgcatcgt tcgcgccgac agcgactacg atgtcaaagc ggaagtacaa aactttctct
    34561 gggctgatgt ggtgatctgg cagatgccag gctggtggat gggcgcgccg tggacagtga
    34621 aaaaatacat tgatgatgta ttcaccgaag gtcacgggac tctgtatgcc agcgatggtc
    34681 gtacccgcaa agatccgtcg aaaaaatacg gttccggcgg cctggtacag ggcaaaaaat
    34741 atatgctttc tctgacctgg aacgcgccaa tggaagcctt caccgaaaaa gatcagttct
    34801 tccacggcgt tggcgttgac ggtgtgtatc tgccgttcca taaagcaaac caattcctcg
    34861 gtatggaacc gctgccgaca tttatcgcta atgacgtgat aaaaatgcct gatgttcccc
    34921 gctatactga agaatatcgc aagcatcttg tggaaatttt tggttaacta gagctcaggc
    34981 tttagaagga gttaaccatg cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac
    35041 atcaccgtca ggcggtattg gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag
    35101 aaggttgcca cggctatgcg ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta
    35161 tggcaccgga ttctatcgtg atgattgagc agtgggaaag catcgcgcat cttgaagcgc
    35221 atctgcaaac cccgcacatg aaggcgtata gcgaagccgt aaaaggtgac gtgctggaga
    35281 tgaatattcg tattctgcag ccagggattt aacattcctg aaaagaggcc ggtgttgcta
    35341 ccggcctcag gtcagattag ccattctttc ttcccgtttt cagcccccga tggaaatcgt
    35401 taattcgctt aatggtttta accgcacgct gcggcaccgc ggaagccacc gaccagacaa
    35461 tcatttccag gcacaacaat accgtaccat gtagtggcgt tttccccttt tcatccccgc
    35521 gcggaacatg aatcacgata ctggcgtctt tgctaaaacg tgaatccagc gcgtttgtca
    35581 gcaaaataac agggatcccc agccgtctcg cttcacgcag cgttgtcagc ccttcccggt
    35641 gcgcagattt ttgcgccatc atgatcagaa catcgccacg ttgaagcgca atcagttgct
    35701 cggcaaggcc aatacccgta cggtttagtg ccgtggcagg caaacctatg cggttgaata
    35761 gtcgtgcggt atagtccgcc agaatacccg aagcgccaat gccaaaaatt gcgacttgcc
    35821 gtgcctgtac caacaacgct accgcttgcg caagcgcgtg gcggttttca ggctctgaca
    35881 ggacattaca ggtatacaaa tgcccttcaa gaacaaagtc gattgcggtg ttaacatcgc
    35941 tcgtcaagtt actcaccgtc gtagacatct tttcgctgga actaagggct ggaccaagcc
    36001 actgttccag agtgcgtttc agatcacgca gcccggcaaa ccccagggcc tgtatagccc
    36061 tgataactgt ggcatcggaa gtgttcaacg tggccgcaat ttccatcgcc gtttgatcga
    36121 gtaccacttc acgatgctca ttaatatagc tggcgacagc tcttaaccca ggcgttaact
    36181 gactcgcgcg agtgcgaaaa cgctcaccat aaatatcaac ccgcgttttg tctttctgaa
    36241 tcatttggcc tccggtaaag gtcgccccgc aatttgtgat tgtatctgcg cagccatcag
    36301 ccccagagat gcaaaggagt tagaaattgc ctcttcacga gaaatcagca cataacgccc
    36361 ggaacgacag gcagtcagaa actgacacca tcccggcatg actttttcca ttgctgccag
    36421 ctcatcctga ggtttaccac cggtgtcacc gcgccaggtt gcaaaaacaa aatcggcatc
    36481 cagttcaggt aaacgctccg cgctcacatc cattcgccca ccttcaggaa tgctctcaat
    36541 cagcggcgga aagcgaaaac cagcatcgcg taatacccgc cccagcgaat gatagctatg
    36601 catcacatta atttttccct gatttgcctg aatcaccgat accgttattt tttggctgtc
    36661 cagcgtcgcc ttcagcgcat tgatttgtgc ctgatagcgg cgttcaagta tcgccagctg
    36721 cgactgcgtt cctgtcagtt ctgccagctt gcgatagatt tctggcgcgc cccctttgag
    36781 atgatcaatg ctcaccgtgg gcgcaatttt ttccagtcgt tcaatgggtg tattacgggt
    36841 cggttctgtg atgatcaggt caggctttgc cgccacaatc gcttcaatat caatatcagc
    36901 ggtgccaata aaagcgatcg atgaattatc aaagtcgaca cctgtcaata acgcgcctga
    36961 tcgaatgaaa tggctaccgt caggccgagt tcgcccgtgg ctggcgacag gaggtacacc
    37021 gagctcaatc agaggaatag tgatatcaag atcgtgtaaa gagacaattc tctttggatg
    37081 agcaggaaca gtgactttgc gccctaaatc atcagtaaag acctgaacag gttctgtcgc
    37141 gcgggcaaag aatgacaaca gtatcagcag agaaaaaaat aaacgcatat aactcccatt
    37201 aaaaactgtt cctgcgctgc cagagcagta gcaaaaagaa tggtccacca atcaatgaaa
    37261 taatgattcc ggcaggcagt tgcaaaggca aaaaagccaa acgaccaatg ctatccgcca
    37321 ggatgactaa caacgccccc gatacggcgc tccccagcag taatgtcgtc tgcccgccac
    37381 gtagaataaa acgtgatata tggggtgcta tcaatccgac aaaaccgatg ttaccaacac
    37441 acgaaacgca gacggctgtc aggattattg gtgcggcgac acgtaacaac gccagacgag
    37501 aactattcac ccctaacccg gttgcaacct gatgaccaag cagagcgata tccgccgtcc
    37561 tggcggtgaa cagcaataaa agagcggctg gcaacatcca acaggctgaa ataccgacta
    37621 acatccagtt agcagcatgc aggcttcccg ataaccacat caatgcagtt tgtacatctc
    37681 gaacatctgc agtggtcata aatacaccga taccggcggc gaaaaaccag gaaacaccaa
    37741 taccaatcag aacaaatcgc gggcgggaaa tttcgcgtgc acaaaaaatc acgattaaag
    37801 ccaccaacaa gccaccagca agaccagcca ctggacgcca gaacattccc agcattggaa
    37861 actggaaaat cagccacagc accgccacgc tacatccttc tttgacgccg attaaaccag
    37921 gatcggcaag gccgttacgc gcaatggatt gcatcgcggc ccccgccata cctaacatgg
    37981 ctccgcatag caatgccatg atgactctcg gcaagcgaat atcctgcaca atataacgtg
    38041 cgtctgtgct tacgttttct ggagcaaaca acgctcgtcc tacttccgat gccgggacaa
    38101 aaaaagaacc atgcatcaga ccgaatatca gtattccaac cgcaaacaga aacagcccac
    38161 caagcaacac cagattttta gggcgtacca gtgtcgaaaa cttaccaaca cgtagtggac
    38221 gtagcccgac gttcattatc cgactcattt gaacattctc gtcgccataa tgacaaaaac
    38281 aggcgcacca actaacgccg tcatgacacc ggtcgctaac tcatggggcg tgaaaagcgt
    38341 ccgagcaata atatctgcca gtaataacaa aagcgcgccg acacaagctg aaagagggag
    38401 cagaacgcgc aaatccgctg ataccagacg cctgacaatc tgcggtacta gcagaccgac
    38461 aaaaccaatg gggccagcaa tcgatacggc tgcgccacag agcaacgcga tcgcaagcag
    38521 agtgaatgtt cgcgtgcgga gtaccgagac gcccagcccc tgcgccatgc gatcacccag
    38581 tgccaacatg ttaagagagg gcgcaaggta aatggctaac acaacccccc ccagagaaaa
    38641 ccaggcggac gtacccagcg tcgcccaatc ctgccccgct aaatcacctg ctagccatgt
    38701 ccgcatcgcc agcagtgttt gttcgtcaag aatcaggatt gcagccgtta ttgaggatac
    38761 gaacgccgat agcgccacgc cgcataaagt aactttcatt ggcgttagcc ctgaacgccc
    38821 tgcggaggaa agcagcaata tcaacaggaa caataacgcg ccgcccgtag atgccagcag
    38881 tgggcgtcca accggaaagg ccagcccaag cgcactggca gcaacgacag caagagcggc
    38941 tccggcattt aagcctaaaa tatgtggctc ccccaatggg ttacgaatga cagcctgcaa
    39001 caaagcacca gcaactccaa gagaagcacc ggtaagcaat gctgccgcta aacgcggcaa
    39061 acggagtctt acaattacgt tgtgatcgaa gttttgcggt tcaaaatgaa gaaatgcctg
    39121 caacaccgtt tgtggtgcaa taaatcgggc accaatccct ggatatacca cgcatcccac
    39181 aagcagtaag gcgaggaaaa ttacagtaac cagccctgtg cgcatcatac tttttctaca
    39241 ccacgaaacg gcatgaagaa cggctttccc gtcagagtat ttattgatgc gtgaacatca
    39301 acgtcaaaga cagcttttac cagctcaggc gtgcaatgct ccccctcatt caacacacgc
    39361 accactttcc cctggcgtaa aaatatcaac gtatcaccat aattgacggc aaaattcaga
    39421 tcatgcaaca ccacgacgac tgtacgcccg tgatgacgcg ttaaatcatg taataactca
    39481 agaatttcaa cctgataacg taaatcaagc caggttgtgg gttcatccag taggatataa
    39541 ggcgtctttt gcgccaacac catcgcaatc cagcaacgct ggcgctgccc accagagagt
    39601 ttttcaacag gtaaatgagc aaactcctgc gtaccggtta atttcagggc ctcttctacc
    39661 gcctgctcgt cggcgtcgct ccattgacgt atgaaatttt gccagggaaa acgcccacgc
    39721 gacaccagtt cataaacggt taagccctcc ggcaataatg gcgactgcgg caagatccca
    39781 agtctacggg ataacgcttt cgttggttgc tcgtgaatcg ctttaccatc cagcaatatt
    39841 gagccgccca tgggttgcaa gacacgtgca atggtgctga gcaacgtcga ctttccacag
    39901 ccgtttgccc caaccagaac ggtcattttt tctgtgggaa cggagaaaga aacaccatca
    39961 acaatgattt tcttgtgata cccggcagaa acattgtcga ggatcagccc ctgccctttt
    40021 gcgttatgtg ccacgtgtac gccaacttaa tcaaagaaaa acaaaaataa aactcattct
    40081 cttttgagag tttgacgcca caaaatgtag tagtcaagaa g
//
LOCUS       DAZFDC010000024        40118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFDC010000024 DAZFDC010000000
VERSION     DAZFDC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.024"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..101)
                     /locus_tag="ACHJ48_001452"
     CDS             complement(<1..101)
                     /locus_tag="ACHJ48_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707040.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLT"
     gene            complement(219..977)
                     /gene="ydjF"
                     /locus_tag="ACHJ48_001453"
     CDS             complement(219..977)
                     /gene="ydjF"
                     /locus_tag="ACHJ48_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4707041.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(1114..2094)
                     /gene="ydjG"
                     /locus_tag="ACHJ48_001454"
     CDS             complement(1114..2094)
                     /gene="ydjG"
                     /locus_tag="ACHJ48_001454"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO4707042.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGFDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEVLGR"
     gene            complement(2104..3036)
                     /gene="ydjH"
                     /locus_tag="ACHJ48_001455"
     CDS             complement(2104..3036)
                     /gene="ydjH"
                     /locus_tag="ACHJ48_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO4707043.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHCTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMNICADMIKPRLNETLDDICEALSYVNYAEAKLLTGKETLDEIADSFLACGVKT
                     VVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNLRECAR
                     FANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(3041..3877)
                     /gene="ydjI"
                     /locus_tag="ACHJ48_001456"
     CDS             complement(3041..3877)
                     /gene="ydjI"
                     /locus_tag="ACHJ48_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO4707044.1"
                     /translation="MLADIRYWENDATNKRYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(3898..4941)
                     /gene="ydjJ"
                     /locus_tag="ACHJ48_001457"
     CDS             complement(3898..4941)
                     /gene="ydjJ"
                     /locus_tag="ACHJ48_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO4707045.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGTLVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGASAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(4958..6336)
                     /gene="ydjK"
                     /locus_tag="ACHJ48_001458"
                     /pseudo
     CDS             complement(4958..6336)
                     /gene="ydjK"
                     /locus_tag="ACHJ48_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(6363..7439)
                     /gene="ydjL"
                     /locus_tag="ACHJ48_001459"
     CDS             complement(6363..7439)
                     /gene="ydjL"
                     /locus_tag="ACHJ48_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO4707046.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(7809..8081)
                     /gene="yeaC"
                     /locus_tag="ACHJ48_001460"
     CDS             complement(7809..8081)
                     /gene="yeaC"
                     /locus_tag="ACHJ48_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO4707047.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8123..8536)
                     /gene="msrB"
                     /locus_tag="ACHJ48_001461"
     CDS             complement(8123..8536)
                     /gene="msrB"
                     /locus_tag="ACHJ48_001461"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO4707048.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8878..9873
                     /gene="gapA"
                     /locus_tag="ACHJ48_001462"
     CDS             8878..9873
                     /gene="gapA"
                     /locus_tag="ACHJ48_001462"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4707049.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9957..10841
                     /gene="yeaD"
                     /locus_tag="ACHJ48_001463"
     CDS             9957..10841
                     /gene="yeaD"
                     /locus_tag="ACHJ48_001463"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO4707050.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWLHDFTLLAHFRVDKNCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10892..11746)
                     /gene="yeaE"
                     /locus_tag="ACHJ48_001464"
     CDS             complement(10892..11746)
                     /gene="yeaE"
                     /locus_tag="ACHJ48_001464"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO4707051.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAVMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11836..12582)
                     /gene="mipA"
                     /locus_tag="ACHJ48_001465"
     CDS             complement(11836..12582)
                     /gene="mipA"
                     /locus_tag="ACHJ48_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO4707052.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNSNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPIVDKSWTGLISTGITYKF"
     gene            13018..14952
                     /gene="yeaG"
                     /locus_tag="ACHJ48_001466"
     CDS             13018..14952
                     /gene="yeaG"
                     /locus_tag="ACHJ48_001466"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO4707053.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15065..16348
                     /gene="yeaH"
                     /locus_tag="ACHJ48_001467"
     CDS             15065..16348
                     /gene="yeaH"
                     /locus_tag="ACHJ48_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO4707054.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            16495..17970
                     /gene="cdgI"
                     /locus_tag="ACHJ48_001468"
     CDS             16495..17970
                     /gene="cdgI"
                     /locus_tag="ACHJ48_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4707055.1"
                     /translation="MILSTRISLGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            18151..19641
                     /gene="dgcJ"
                     /locus_tag="ACHJ48_001469"
     CDS             18151..19641
                     /gene="dgcJ"
                     /locus_tag="ACHJ48_001469"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO4707056.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSILQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            19684..20187
                     /gene="yeaK"
                     /locus_tag="ACHJ48_001470"
     CDS             19684..20187
                     /gene="yeaK"
                     /locus_tag="ACHJ48_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO4707057.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(20188..20292)
                     /gene="yoaI"
                     /locus_tag="ACHJ48_001471"
     CDS             complement(20188..20292)
                     /gene="yoaI"
                     /locus_tag="ACHJ48_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO4707058.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            20462..20908
                     /gene="yeaL"
                     /locus_tag="ACHJ48_001472"
     CDS             20462..20908
                     /gene="yeaL"
                     /locus_tag="ACHJ48_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO4707059.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIATGLVSLIVGKQ"
     gene            complement(20865..21686)
                     /gene="nimR"
                     /locus_tag="ACHJ48_001473"
     CDS             complement(20865..21686)
                     /gene="nimR"
                     /locus_tag="ACHJ48_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO4707060.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     YTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPWQ"
     gene            21783..22964
                     /gene="nimT"
                     /locus_tag="ACHJ48_001474"
     CDS             21783..22964
                     /gene="nimT"
                     /locus_tag="ACHJ48_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO4707061.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            23019..23366
                     /gene="yeaO"
                     /locus_tag="ACHJ48_001475"
     CDS             23019..23366
                     /gene="yeaO"
                     /locus_tag="ACHJ48_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO4707062.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(23388..23642)
                     /gene="yoaF"
                     /locus_tag="ACHJ48_001476"
     CDS             complement(23388..23642)
                     /gene="yoaF"
                     /locus_tag="ACHJ48_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO4707063.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRLDHPAGEK"
     gene            23825..24850
                     /gene="dgcP"
                     /locus_tag="ACHJ48_001477"
                     /pseudo
     CDS             23825..24850
                     /gene="dgcP"
                     /locus_tag="ACHJ48_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
     gene            24883..24981
                     /gene="yoaK"
                     /locus_tag="ACHJ48_001478"
     CDS             24883..24981
                     /gene="yoaK"
                     /locus_tag="ACHJ48_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO4707064.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            24984..25058
                     /gene="yoaJ"
                     /locus_tag="ACHJ48_001479"
     CDS             24984..25058
                     /gene="yoaJ"
                     /locus_tag="ACHJ48_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO4707065.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(25117..25365)
                     /gene="yeaQ"
                     /locus_tag="ACHJ48_001480"
     CDS             complement(25117..25365)
                     /gene="yeaQ"
                     /locus_tag="ACHJ48_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4707066.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(25512..25694)
                     /gene="yoaG"
                     /locus_tag="ACHJ48_001481"
     CDS             complement(25512..25694)
                     /gene="yoaG"
                     /locus_tag="ACHJ48_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO4707067.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(25698..26057)
                     /gene="yeaR"
                     /locus_tag="ACHJ48_001482"
     CDS             complement(25698..26057)
                     /gene="yeaR"
                     /locus_tag="ACHJ48_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO4707068.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(26230..26868)
                     /gene="leuE"
                     /locus_tag="ACHJ48_001483"
     CDS             complement(26230..26868)
                     /gene="leuE"
                     /locus_tag="ACHJ48_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO4707069.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGMATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(26995..27918)
                     /gene="dmlR"
                     /locus_tag="ACHJ48_001484"
     CDS             complement(26995..27918)
                     /gene="dmlR"
                     /locus_tag="ACHJ48_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO4707070.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            28021..29106
                     /gene="dmlA"
                     /locus_tag="ACHJ48_001485"
     CDS             28021..29106
                     /gene="dmlA"
                     /locus_tag="ACHJ48_001485"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO4707071.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            29357..30967
                     /gene="yeaV"
                     /locus_tag="ACHJ48_001486"
     CDS             29357..30967
                     /gene="yeaV"
                     /locus_tag="ACHJ48_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO4707072.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            30999..32123
                     /gene="yeaW"
                     /locus_tag="ACHJ48_001487"
     CDS             30999..32123
                     /gene="yeaW"
                     /locus_tag="ACHJ48_001487"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO4707073.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            32179..33144
                     /gene="yeaX"
                     /locus_tag="ACHJ48_001488"
     CDS             32179..33144
                     /gene="yeaX"
                     /locus_tag="ACHJ48_001488"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO4707074.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTPVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHQSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(33198..34313)
                     /gene="rnd"
                     /locus_tag="ACHJ48_001489"
     CDS             complement(33198..34313)
                     /gene="rnd"
                     /locus_tag="ACHJ48_001489"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO4707075.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(34395..36080)
                     /gene="fadD"
                     /locus_tag="ACHJ48_001490"
     CDS             complement(34395..36080)
                     /gene="fadD"
                     /locus_tag="ACHJ48_001490"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO4707076.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTLVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVSPGQPGELCVRGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(36285..36866)
                     /gene="yeaY"
                     /locus_tag="ACHJ48_001491"
     CDS             complement(36285..36866)
                     /gene="yeaY"
                     /locus_tag="ACHJ48_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO4707077.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGHGGWGWYNPGPARVQTVVTE"
     gene            complement(36906..37601)
                     /gene="tsaB"
                     /locus_tag="ACHJ48_001492"
     CDS             complement(36906..37601)
                     /gene="tsaB"
                     /locus_tag="ACHJ48_001492"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO4707078.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(37659..39569)
                     /gene="yoaA"
                     /locus_tag="ACHJ48_001493"
     CDS             complement(37659..39569)
                     /gene="yoaA"
                     /locus_tag="ACHJ48_001493"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO4707079.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            39701..40045
                     /gene="yoaB"
                     /locus_tag="ACHJ48_001494"
     CDS             39701..40045
                     /gene="yoaB"
                     /locus_tag="ACHJ48_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4707080.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT        10144 a        10168 c         9414 g        10392 t
ORIGIN      
        1 gttaataaca gactaaagct tataatacca aatatacgat aatgaaaccg ggccaaaggc
       61 aagcggtcca gtcttgcgcc aatttgatca tattgttcca ttgggtgcct cggatttgct
      121 aaaaaaggta gcaattgcgg caaattcact tgccgcatct ttatcatcat tttatttcca
      181 ggcgcaggaa caaataatat tgacaacgcg tgcttaaatt accacaccag ctggatatta
      241 ttgtctttgc aaaatgctat ccactctgca cccggtgatt tatcagttat tatgtaatta
      301 atatgactaa agtcggctaa ctggacaaaa gctttgcgat caaacttaga gtgatcaacc
      361 aataacgcaa cttctgtcgc ctgacggatc attgtctttt tgatttcagc ttccgcttca
      421 ttagagtcca gcgcgccgct gttaatatca agacctttac aactcattac catgatatcg
      481 acatgatagc ggctgatgat ctctttagta attcttcctt gcagtgaaag tgtatttttg
      541 tttagttccc cacccgttga aacgacttta atttctgact gagccagaac atgaatcgct
      601 tccgccgagt tggttagcaa cgtcaggtca ctacggtcct gtaacaattt gagcaattcc
      661 attacggtac tactggaatc ggctgccatg gtggttttat tgtcgataaa gggtagtgcc
      721 ttgcgtgcaa taagctgctt ctcttcataa aacgatgaag cgcgcttata aaaatggata
      781 ttctccgtca acatcgctgt atttaaaaca gcaccaccat aggttctggt caaaaagcct
      841 tcatcttcca gcttctcaag atcgcggcga atggtttctt cggttacctg aaaaatccca
      901 ctcaaatttg agactgtcac ctttttatcg ttggcaacca tttgcttaat tgcctgaatc
      961 ctgtcttttg ccgccacgat tacacccctg tatcttttta catcacatta gcgcgattat
     1021 cgcataaccg atgtttactt tcaaaataac ctgtttgaat cacagatttt catcacagtt
     1081 ttcacagaaa cagaggtgaa tcgtgttgag tatttaacgc cccaagacct ctgccatttc
     1141 cctcatcaat gttgcgtctg catccgataa gttgatattc agtgccgcga cattttcgcg
     1201 tacctgttcc ggtgcagtag ccccactaag aatggagatt aaatcactct gttttaatat
     1261 ccacgccagt gccagagtgg gaattgtgca ctgataacga gcacaaagtg gctgccactg
     1321 ttcaagcata tcaatcactt tcagcatgtt ttcacgctgg aaccagactt tatttgcccg
     1381 agcgccgccc ggaacgtaat cacgagtgat ggtgccggtc aacaatccct gctctagcgg
     1441 ggaataaacc tgaaccacaa tgccattatc acgacatagc ggcagcagtt cgttttccat
     1501 tgcccggtcg aggatactgt atttcgcctg aataatatcc agttcaccat attgcagata
     1561 ctcgcggata tggtcagcat cgacgttagc agcgcctata gcgcgaattt tcccttcagc
     1621 ctttaactca ttaagcactg cgacagtttc agcgatcggc gtataaaatg gcggcaccga
     1681 ctgccagtgc gtcatgtaga tatcgatgta atcaaaaccc agacgttgca agctggcttc
     1741 tacctcttcg cggatagatt ccggggaaag gtttttatac aactgccgat cgccaacttt
     1801 gttgaataaa cttccttttc gttcccagac aatgccgcat ttggtttcta ctacaacctg
     1861 ttcacggggc agttttttta acgcctgacc gacgataact tcactattgc caaagttata
     1921 tcctggtgca gtatcaatca ggttaatgcc gcaacgatgg gcttcaagaa tcgtatcaat
     1981 acatatttgc cgatcgagat cgccattcca tgcaggaccg ccgccaatgg cccatgtccc
     2041 caaccccatt cgcgaaagcg taatatccgt tgtgcctaaa ggtatctttt tcatttgcct
     2101 tcattatcct tcgtattctt ccagcaattg ttcgaccagc tttctgtttt ttacgccggt
     2161 ggtggcaccg acgcttagaa ccgagatagc cgccgttgca ttggcaaagc gtgcgcattc
     2221 acgcagattt tttccttcta acagtgccgc aataaaacct gaagcaaagt tatcgcccgc
     2281 gccaatggtg tcgatggcgg ttattcctgc gaccgccggc accttcatcg tcatgtcacc
     2341 acgcttgata aagcagccgt ctttacccgt tttaatcacc accgttttta cgccgcacgc
     2401 aagaaagctg tcagcaattt catccagtgt ctctttcccg gtgagtaatt ttgcctcggc
     2461 ataattgaca taactcaatg cttcgcaaat atcatccagc gtttcattca accgcggttt
     2521 aatcatatcg gcacagatat tcatctgccg ggctttagct tgcgtaaaaa tttctgttag
     2581 cgctttacca tccaatagtg gactgttgaa aatactggcc agtgataata atttcgcctg
     2641 ggaaaaccgc gcaaaatcaa catcgtcaat attcaatttc cacagactgc cgttacggtt
     2701 tgtgacaaac gtccgctcgc catcctccgt caccagtcca acattaatag aggtatctat
     2761 gttgacgtcc tgcttcaggc tttgaatatc aatattctct ttgcggcaat ggtcgagaat
     2821 aaactgccct gcggcatctt taccaatacg gctcattaat gctgtacaat ggcccagacg
     2881 agaaataatt gttgcttcat ttattgcatc acccccggtg gtcattgcga ttctttcaag
     2941 aggataagaa tccacatcaa agatattttt actgaccggt tgcaatggaa tatcaacaat
     3001 agcggcacct atacaaataa cgtcgagatt atccatatca ttattccgct ttgccatcag
     3061 aaccgaacag tttaattttt tccaatgccc gttcttttac agctttacgt acttcacgtt
     3121 ctaaatgcag gaaaggctga tcctggtttt ctttcactgc gaccattgcg gcctgacaga
     3181 gctccgtatg gatgttgatt ttcgcgatac ccaacgaaat tgcagtttta atgtcggcat
     3241 cactgatccc cgatgcgcca tgcaaaacca gcggaacaga cactgcatcg cgtacgcgtt
     3301 tgacgacctc aaagttcaat tgtggctctg acgtataaac cccatgctgg ttgccgatgg
     3361 cgacagccaa tgagtcacag cccgtacgtt caacaaattc agccgcctgg tcaggatcgg
     3421 tatagtgata gcccgccagc gcttcctcat aaacggtttc attaccgaca tgccctaatt
     3481 ccgcctctac cggaataccc agcggatgga agaaatcaac agcctctttg gttaaacgaa
     3541 tattttcttc gaaatcaaat gcggaagcat cacgcattaa tgaattcata ccatgagtcc
     3601 aggcgttatg aataatctcc atactccgac catgatccca atgagttatt accggcaccg
     3661 ttgctttttg tgccatagat accatcatgt gagagaaatc ttcaaatgag gtgttgccga
     3721 caaaacctgt gccaaaagaa ataataaccg gtgatttcgc ttcttcggct gcgtcgataa
     3781 cgcccatcaa catttctgca ttccatacat taaaatgggc aatagcataa cgtttatttg
     3841 tggcatcgtt ttcccaatat ctgatatctg cgagcatgtt ctgttctccc gtattaatta
     3901 atcgctaatc ttaataacgc ctttaataat gtcgcgtttg ttgttaactg actcttcaaa
     3961 tgcctgttgt acatcccgat aatcgtaaat atgcgtcacc atcgatttca catcgaatcg
     4021 ccctgaagaa attgcttcaa tcgtgaccgg ataacgattg gcatagcgga ataccgtctg
     4081 gatagtgact tcgcgattga ttttgaggaa attgattgcc gaagcgccgg gtacagtacc
     4141 aacaatcata attttaccgc cacgcattac cagataaggt gcctgtttaa cggtgaccgc
     4201 agaacccgct gtttcgaaaa caatatctgc gcccatgtct tcggtaaatt gctgacagcg
     4261 tgcaatagtg tcttcttttg cgccgttaat aaccactgtc gcaccaagct gttccgccat
     4321 tgccagacgt ttttccagca catcaacgac ggcaatttcc gttgctccca ggcatttgca
     4381 cgcttgcaac gtcatcaaac caatacaacc tgctcccaga ataattatct tcttacccgg
     4441 tttaacatct gccagcatcg cggcatgcat cccgactgcg gcaggctcca ccagcgtccc
     4501 ttccatcgtg tccatattgt cgggcagttt gtaagtaaag ctctccggat gacacagata
     4561 gtgcgttaat gcgccgcggt agttgggttg tgtcgccata aaatcaacgt ccgggcagat
     4621 gttatatttg ccttccagac agtaacgaca gtgaccgcaa ggaacgccag gttcgatatt
     4681 tacccgatcc cccggtttaa atttgcgcac acggcttccc acagccacaa ccgtcccggc
     4741 gcattcatga cccaggccaa tttcttgatt tgggtcttta ggcggaataa acggacctga
     4801 ttcaaaacca tgtacatctg aaccacaaat accgacatat tctactttaa tcaaaacttc
     4861 atcttcttta ggcactggta tttctgctga aataattttc attgtgcccg gcacctgcaa
     4921 tattgctttt gaatttttca ttttgaatcc acccgtatta tttattggct actgcatcaa
     4981 tacttaaact ttcaacggag acacctttgg tttcgattcc aatggtggcg atagcaattg
     5041 cgacaataat tgaaaccgcc cccagaagaa taaagactcc cgtaacgcca taactactga
     5101 gcagcactgc aacggcataa ggtgcggcaa taccactgat tcgccctacc gcattcgcca
     5161 gaccggagcc acggagtttg gcctctgtcg gccagatctc agggacatac actgccgagg
     5221 catagcaaac gtacatatag acgaaagtaa tcaggaagaa accaatcaac gttatgagca
     5281 acatgctggt ttgcagtgaa tagatatatc cgagcaccgc aatcaggatt aatagcccca
     5341 cacccattgt tttacgcgga attttatcca tcaccagcat ggcaataaaa atgccaaatg
     5401 gcgtgccaaa catactcatg gtatttaaaa caatcgagtc ttttaaatta atcccctggg
     5461 tcatgaatat cgttggcaac cagttaatta gtgtgtactg cacaacgttc atggcaatca
     5521 gcacacaaga gcctaatatc acgcgtttca gtaatactcc tgtcagtaag gctgaatatg
     5581 gcaccgcttg tggtgcttta ccgtcatcag caataaccac gggcggcaaa ggtttacctg
     5641 tatggcgtat gacgccttct tctatactcc gcatgacttt ctcggcttcc tgataccgtc
     5701 cgcgcgattc cagccagcgc ggggattcag gaaagtagcg ccaggccagc gccgtagcga
     5761 taagcgacaa tattgcaggg ataagcagtt gtacccgcca gttccactct gcactaatca
     5821 gcggcgtgag tcccatcgct atcaatgaac agagcggata tgaccagttg ccaataaagg
     5881 aaacccgact tgaccacgtt ccacgatttc taccgggcat atattcggtg aaccagcaaa
     5941 cagcgtaacc agtaaagctc ccagcccaac tcccatcaca aaacggcagg caataaggaa
     6001 atccatattc ggtgagaaag caccgacaac catcgaggca atatgaatgg cctcatagag
     6061 gatgaacgca tttctgcgac cggttttgtc gccaatgatg ccgcccacca gcgcaccgag
     6121 aaacattccg gcggtcgtga ttgctgagaa tgtggctgtg gtggaattat ctgtccagcc
     6181 caacgctttc agctgcgcga ggatcaagcc accaacggca ttactccagc agacaagcaa
     6241 gccaaacgcg acgatggcaa acattgatga atgccagcgg caatccggta agcgatccag
     6301 ccgtgcacca caatgcggtt ttgttatctg atccatgcga taacgttcct tcaggagagt
     6361 ggttattcat caaagtcgta agtcatgatc actttgattg cggttttatc gaccatcgca
     6421 tcaaaccctt cgcgccattg cgacaggccg atacgatgcg tgatcatcgg tttgactttg
     6481 atagcgccgc tggctaatag cctgatagcg ttacgccatg aggtggagtc ataggccata
     6541 tgcccaatga tgcttttgtt ccaggcggta atgtcattaa tcgagaaatc aagaggtttg
     6601 aagcccattc caacgcgtac cacttccccg ttcggacgga gcatttcgat ggcttgtttc
     6661 agtgcgatat tggcaccgga gcattcaatc accagtccca gattgtcttt gccacaaatt
     6721 tgctggcagc gcgccaccac atcttcggta gaaccattta ctactgccgt cgcacccagt
     6781 tcttttgcaa ccgggaagcg gaccgccaca tcttcttgca gaccaacgac gacgatattt
     6841 accgccccca taattcgcgc catttgtacg gagaacagcc cgagtgggcc agtgccgatg
     6901 acgaccacat cctgaccagg gaggaatttc gattgctgcg cgatggattt gtaggcatta
     6961 cagatagggt caagtacggc tgcgtcctca taatcaacac catcagggat ttcccacaac
     7021 gcatgacgat gaattttgag aatttcacca ggaaccagac aatatttgga aaaaccaccg
     7081 ccccaggtat tgttatccag accaaggttt actttttctg tacaacacag aaaatcacct
     7141 tgttcacagg ccggacaaac gccgcagacg tgaccgctgt tatccgacac gacgcgttgc
     7201 cccactttcc agtctttgac tttttcacca acctgcgcaa tacaacctgc gaactcatgg
     7261 ccgcggatag agttaaactc atcagaaccg ctatcgacat tgtagtgctt catgtctgcg
     7321 ccgcagattg ccgcggcttt aatttcaatc actacatctt ccgggccaca aatgggttgt
     7381 ggcacatcga tcatcttgta gccgccaaag gctttgccaa accgagccag tgctttcatt
     7441 gcgtcatcct ctgtgttttg ttttgcttag atggtcatca tacttaccca catgattttt
     7501 gaggtcaaca ttaaacatgt aaaacacaga taataatctg cgttttacaa ctcagatcac
     7561 aattacgcag aaaatggctg gttctgttac aggttgatgt aaggcggaac gcaaaaggag
     7621 caaatttgag agggattacc ggccaaatag gtctgagact taagttcagg agaagtcatt
     7681 caggcgtgaa cgcgacggca agagatcgga ctgggactta gccaggtcat ggcgagataa
     7741 ttgagatgag tcgtggggtc attgttggct atccccttca tagccgcggc aaaaagggga
     7801 tgaaccactt acttaaacat ggcaataggc tttgctgaga taccaaaatc ttcttttaac
     7861 tgctgtttgc tcttcatgac catctgacca tttgtgtcaa ttgtcatatg ctgtgcttcg
     7921 gtattattgc gggcttgcca cagcatcacc agttgcaggc agttctcttt ttgttcctcg
     7981 gtcaacgcaa cgccatcagg ccatttcccc agttcaacgg cggtcgacaa acgctggtat
     8041 acctcaggcg tcatgctgtt gataatgtca tcaagattca taatttttcc gctcctgtgg
     8101 aataagttgc tgaatcgttt tttcaaccgt tgatttcttc gccgttttca ccatcagtga
     8161 agcgtaaaga agcagagtta acacaataac gttcgcccgt tggctgcggc ccgtcaggga
     8221 agacatgccc cagatgggca tcacagttac cgcaacgaat ttctatgcgc tgcattccat
     8281 gtgacaagtc tttgatataa cgaatggatt cttcactcac cggttcgtag aaactgggcc
     8341 agccacagcc ggaatcatac ttggtttggg aatgaaacag cggggcatcg cagatcaaac
     8401 agtgatatac gccgtcacgc ttgttatgca gtaaacgacc cgtaaatggc ggctctgtcc
     8461 catgattctg cgtcacgtaa aactgcatct cggacaaatt ttttttcagt tcttctgccg
     8521 aaggtttatt agccatttgc tcacatctca ctttaatcgt gctcacatta cgtgactgat
     8581 tctaacaaaa cattaacacc aactggcaaa attttgtcct aaacttgatc tcgacgaaat
     8641 ggctgcacct aaatcgtgat gaaaatcaca tttttatcgt aattgccctt taaaattcgg
     8701 ggcgccgacc ccatgtggtc tcaagcccaa aggaagagtg aggcgagtca gtcgcgtaat
     8761 gcttaggcac aggattgatt tgtcgcaatg attgacacga ttccgcttga cgctgcgtaa
     8821 ggtttttgta attttacagg caacctttta ttcactaaca aatagctggt ggaatatatg
     8881 actatcaaag taggtatcaa cggttttggc cgtatcggtc gcattgtttt ccgtgctgct
     8941 cagaaacgtt ctgacatcga gatcgttgca atcaacgacc tgttagacgc tgattacatg
     9001 gcatacatgc tgaaatatga ctccactcac ggccgtttcg acggtaccgt tgaagtgaaa
     9061 gacggtcatc tgatcgttaa cggtaaaaaa atccgtgtta ccgctgaacg tgatccggct
     9121 aacctgaaat gggacgaagt tggtgttgac gttgtcgctg aagcaactgg tctgttcctg
     9181 actgacgaaa ctgctcgtaa acacatcacc gctggtgcga agaaagtggt tatgactggt
     9241 ccgtctaaag acaacactcc gatgttcgtt aaaggcgcta acttcgacaa atatgctggc
     9301 caggacatcg tttccaacgc ttcctgcacc accaactgcc tggctccgct ggctaaagtt
     9361 atcaacgata acttcggcat catcgaaggt ctgatgacca ccgttcacgc tactaccgct
     9421 actcagaaaa ccgttgatgg cccgtctcac aaagactggc gcggcggccg cggcgcttcc
     9481 cagaacatca tcccgtcctc taccggtgct gctaaagctg taggtaaagt actgccagaa
     9541 ctgaatggca aactgactgg tatggcgttc cgcgttccga ccccgaacgt atccgtagtt
     9601 gacctgaccg ttcgtctgga aaaagctgca acttacgagc agatcaaagc tgccgttaaa
     9661 gctgctgctg aaggcgaaat gaaaggcgtt ctgggctaca ccgaagatga cgtagtatct
     9721 accgatttca acggcgaagt ttgcacttcc gtgttcgatg ctaaagctgg tatcgctctg
     9781 aacgacaact tcgtgaaact ggtatcctgg tacgacaacg aaaccggtta ctccaacaaa
     9841 gttctggacc tgatcgctca catctctaaa taagttgaga tgacactgtg atctaaaaag
     9901 agcgacttcg gtcgctcttt tttttacctg ataaaatgaa gttaaaggac tgcgtcatga
     9961 ttaagaaaat ttttgccctt ccggtcatcg aacaaatctc ccctgtcctc tcccgtcgta
    10021 aactggatga actggacctc attgtagtcg atcatcccca ggtaaaagcc tcttttgcat
    10081 tacagggcgc acaccttctc tcgtggaaac ctgcaggtga agaagaagtt ctgtggttga
    10141 gcaacaacac accgttcaaa aatggcgttg ctattcgcgg tggcgtaccg gtttgctggc
    10201 cgtggtttgg tccggcggca caacaaggcc tgcctgcgca cggttttgcc cgcaacctgc
    10261 cgtggacgct gaaatcacat cgtgaagatg ctaatggcgt agcgctgact tttgaattga
    10321 cacaaagcga agagacgaaa aaattctggc tgcacgactt tactctgtta gcgcatttcc
    10381 gcgtggataa aaactgtgaa atcgatcttg aagcacacgg cgaatttgaa accacctctg
    10441 ccctgcatac ctactttaac gtgggtgata tcgctaaggt aagcgtcagt gggctgggcg
    10501 atcgcttcat tgataaagta aatgacgcga aagaagatgt actgaccgac ggtattcaga
    10561 ccttccctga ccgtaccgat cgcgtgtatc tgaatccgca agattgcagc gtgattaatg
    10621 atgaagcgct gaatcgtatt atcgccgtag gccaccagca tcatctgaac gttgtaggct
    10681 ggaacccggg accggcgctt tcagttagca tgggcgacat gccggacgat ggctacaaga
    10741 cctttgtttg tgtagaaaca gcttacgcct cagaaatgca aaaagtgacc aaagaaaaac
    10801 ctgcacatct ggcgcaatcc attcgcgttg ccaaacgtta atttgcgtca atgttgtgtg
    10861 ccgggtgcga tgcatccggc acgtatcaac atcacaccat atccagcgca gtttttcctt
    10921 ttggtgccgg atacgctttg tccagcatgg ctaattccgc tgacgaaagt tcgacctcaa
    10981 gcacagccgc attctgttgg acatgggcaa tcgtggccgc ttttggaatc gccatcacac
    11041 cctgatggct gattacccac gccaacaata cttgcgccgc gctgatattg tgagcatgtg
    11101 caatttcgtt gactaccgcg ttttttaaca gtccattgcg caaccgcccg gcctgggcta
    11161 acggactgta agccatcacc ggcatctgct gttgctggca ccaggggagt agatcgtact
    11221 caatccctcg tgaaccgaga tggtaaagca cctgattagt ggcacactga tttccccccg
    11281 gcagctgcca gagttcctgc atatcagcat aatcaaggtt cgaaacgccc cagcggcgga
    11341 tttttccctg ggcgatcaat ttttccatca ctgcaacagt ctcttcaaaa gcgaaactgc
    11401 cagaccagtg taataagtag agatcgagat aatcagtatt gagacggcgt aaactggctt
    11461 cgcatgcatt tatcgctttt tgcccgccag cattccacgg atagacttta gagacgagaa
    11521 agaccttctc tcgcagaccg gttaatgctt ccccaaccac cttttcggca ccgccatcgg
    11581 catacatttc ggcggtatca atgagggtta aaccgagttc aatgcccgcg cgtagtgcag
    11641 caacttctgt tttgcgctga ctggcatctt cgcccatata ccatgttccc tgccctatgg
    11701 ctggcagtga gacatcgcca ctaaattgaa tcattttttg ttgcattgtt tcctcccagg
    11761 tattgcacca ccgtaatgca aaacagggcg tgacgccctg tttttatgca caaaatgccc
    11821 tggaaagatg cattatcaga atttgtaggt gatcccggta gaaatcaggc cagtccagga
    11881 tttatccaca atcgggctgt cagtaacttc atcagacaga cgggtgtagc gcgcggtacc
    11941 gtaaacactc cagtcgccga ggaagttgta gctggcgctc agctccaggt aagggctcca
    12001 gctgtcgttc gagttatagc cacgcagacc gctgcgagcg gactctttgc gcgatacgcc
    12061 ataatagtat tcgttctggt tttcgctgtt ccactgcaca ccaatacccg gagtcacggt
    12121 caggccaccg ttggtgtaac gatacaacca ggccatatcc cagacgatgc cgttgctgtt
    12181 atccagggta tcgccagcca gggtggtacg caggtaaccg tactgggtaa agtgagcata
    12241 agacagacca gccatcatgg tgctcttacg gtcatccagg tgacgcattt ggtgatcgcc
    12301 actgtcttta gctttgaagt aaagcggcga ccagtaagcg gtaattgaaa gtttatccgt
    12361 tgcgtcattc cacaggtagt aaccaccacc taagccacgg aaccagaagt tatcgccttc
    12421 atagttgatt accggtactg ggtaaacatc ggtatcgtaa tctttatatg ggtgttcaac
    12481 gacacctacg cctgcgccca gggaaaattt accttcagcg tgcgctacgc ctgcagacgt
    12541 tgcgataagc actccaagtg ccagaagttt gagtttggtc acaattaatc attccttaaa
    12601 caaatgttta gcgggcgaca aagtttaccc gtcaatacat cgaaacccaa cctttttacg
    12661 ttttcatttt ttaaagtaac tgtttaattt tcctgacgcg gatgacaccg cgcttacagc
    12721 caaatgaatt tagcgttact ggcgagcctg gtctttacat taattatgca aaatttatgg
    12781 atgagttgtt gatatgccat tgaaattaag aaagccgtgt aggcaagttt tccatttgcc
    12841 atctacgctt aattttgaag gtgtatcacc gggcacgttg ttctcatcgt cgataaaatg
    12901 gcatgagagt tgctgtgttt tagcaagaga cgtcgttcag tttacctctt ccgggagcct
    12961 ctactattca tatgaacggc tcttaacctg tgctaaaaaa cgaaaggacg gcataccatg
    13021 aatatattcg atcactatcg ccagcgatat gaagctgcca aggacgaaga gttcacactg
    13081 caggagtttc ttaccacttg tcggcaagat cgcagtgctt atgccaacgc ggctgagcgg
    13141 ctattgatgg ctatcggtga gcctgtcatg gtcgatacag cccaggaacc cagactttct
    13201 cgactctttt ctaaccgggt cattgcacgt tatccggcgt ttgaagagtt ttacggcatg
    13261 gaagacgcga ttgaacagat tgtctcttat ctgaaacacg cggctcaggg gctggaagag
    13321 aagaaacaaa tcctgtatct gctggggcct gtgggtgggg gtaaatcatc gcttgctgag
    13381 cgactgaaat cattaatgca gctcgtaccg atttatgtat tgagcgccaa cggtgagcgt
    13441 agcccggtca acgatcatcc gttctgtctt ttcaatccgc aggaagatgc gcagattctg
    13501 gaaaaagagt atggcattcc tcgccgttat ctcggcacca tcatgtcgcc gtgggcggca
    13561 aaacgcctgc atgaatttgg tggcgatatc actaagttcc gggtagtgaa ggtctggccg
    13621 tcaattctgc aacaaattgc tatcgccaaa acggaacccg gtgatgagaa caaccaggac
    13681 atctccgcgc tggttgggaa agtcgatatt cgtaaactcg aacattacgc gcagaatgac
    13741 ccggacgcct acggctattc cggtgcgctg tgccgcgcca accaagggat catggaattt
    13801 gtcgagatgt ttaaagcacc gataaaagtg ctgcatccct tgttaaccgc cactcaggaa
    13861 ggtaactaca acgggacgga aggtatctcc gccctgccgt tcaacgggat tattcttgcc
    13921 cactcgaacg agtccgaatg ggtcactttc cgtaataaca aaaacaacga agccttcctc
    13981 gatcgtgttt acatcgtgaa ggtgccgtat tgcttgcgca tttccgaaga gatcaaaatc
    14041 tacgagaaat tgcttaatca cagtgaattg actcacgccc catgcgcccc tggcactctc
    14101 gaaacactgt cacgtttttc cattctttcg cgcctgaaag agccagaaaa ctccagcatt
    14161 tattcaaaga tgcgggttta tgatggcgaa agtctgaaag acaccgatcc caaagccaag
    14221 tcgtatcagg aatatcgtga ctacgccggt gtcgatgaag gtatgaacgg tctgtcgacg
    14281 cgttttgcgt ttaagatcct ctcccgcgtg ttcaacttcg atcatgtaga agtggcggca
    14341 aacccggtcc atctgttcta cgtcctggaa cagcagatcg agcgcgagca gttcccacaa
    14401 gagcaggcag aacgctatct ggagttcctg aaaggttatc tgatcccgaa atatgccgag
    14461 tttattggca aagagatcca gacggcctac cttgaatcct attccgaata tgggcaaaac
    14521 attttcgacc gttatgttac ctacgcggat ttctggattc aggatcagga gtatcgcgat
    14581 ccggataccg ggcagctgtt tgaccgcgag tctcttaacg ccgagctgga gaaaatcgag
    14641 aaaccggcgg ggatcagtaa tccaaaagat ttccgcaacg agattgttaa cttcgtactg
    14701 cgcgccagag ccaataacag cggacgcaat ccgaactgga ccagctatga aaaactgcgc
    14761 acggttatcg agaagaaaat gttctccaat accgaggagc tgttgccggt tatctcgttt
    14821 aacgccaaaa cgtcaaccga cgagcagaag aaacacgacg actttgtcga ccgtatgatg
    14881 gaaaaaggct acacccgtaa acaggtgcgt ttactgtgcg aatggtattt gcgcgtacgt
    14941 aaatcgtctt aacaaccctg gcccggtaca catgctaccg ggcctacaac gacagcgaac
    15001 cgtgggccag agaaatggca acacaggcgt agcatacagt tggcaaatgt agtacggggg
    15061 gcatatgacc tggtttattg accggcgtct gaacggcaaa aacaaaagca tggtgaatcg
    15121 ccagcgtttt ttacgccgtt ataaagcgca aattaaacag tcgatctccg aggccattaa
    15181 taagcgttcg gtgactgacg tcgacagcgg cgagtccgta tccattccca cggaagatat
    15241 tagcgaacca atgtttcatc aggggcgtgg cggtctgcgc caccgcgtgc atccgggcaa
    15301 tgaccatttc gttcagaacg accgaattga acgtccccag ggtggcggcg gaggttccgg
    15361 cagtggtcag ggccaggcca gccaggatgg tgaaggtcag gatgaatttg tctttcagat
    15421 ttcgaaagat gagtatcttg atctgctctt tgaagatttg gccttaccga atctgaaaca
    15481 aaaccaacaa cgccagctga ccgaatataa aacgcatcgg gcgggttata ccgctaacgg
    15541 cgttccggcc aatatcagcg ttgtgcgttc cttgcagaac tcactggcgc gacgcacagc
    15601 catgacggca ggcaagcggc gggaacttca tgcactggaa gagaatttgg ccatcatcag
    15661 caacagtgaa cctgcgcagc tgctggaaga ggaacgtctg cgcaaagaaa ttgcagaatt
    15721 acgtgccaaa attgaacgcg tcccttttat tgacaccttc gatttacgtt acaagaacta
    15781 cgagaagcgg cccgatccct ccagccaggc agtgatgttt tgcctgatgg acgtttccgg
    15841 ttcaatggat caatccacta aagatatggc taagcgtttt tatattctgc tgtatctgtt
    15901 cctcagcaga acgtataaga acgtggaagt cgtatacatc cgccatcata cccaggcgaa
    15961 agaagtcgat gaacatgagt ttttctactc gcaggaaact ggcggcacca ttgtttccag
    16021 cgccctgaaa ctgatggatg aggtagtgaa agagcgttat aacccggcac agtggaatat
    16081 ttacgctgca caagcatcgg acggcgataa ctgggccgat gactctccgc tttgccatga
    16141 aattctggcg aaaaaattat tacccgttgt tcgttattac agctatatcg aaattacccg
    16201 tcgcgcgcat cagacactgt ggcgagaata tgagcatctg caatctactt tcgacaactt
    16261 tgcgatgcag catatccgcg accaggatga tatttatccg gtgttccgtg aactgtttca
    16321 taagcaaaat gcaacagcta aagactaaaa ctatcagccg ggtcattatc gcctggctga
    16381 tttttagctc actgtaagtt atctcattta ttacatacat tagcttacaa tcgctttaaa
    16441 tatgacagca taacctttac acaatttagt tccagaaaac aatcattcgg aaaaattatt
    16501 ctgtcaacac gtatttccct ggggttattc tttaaatatt ttttatcgtt aacgaaaatt
    16561 gatcctggtc aaaactatat atctctgcca tcaataaaat ccagcactca cattgctctc
    16621 ctttttatgg tttctatggg tacacaaaaa ttaaaagctc aaagcttttt tattttcagt
    16681 ttattgctga cgttaatttt attttgcatt actaccttat ataacgaaaa cacaaatgta
    16741 aaactcatcc cacagatgaa ttacctgatg gttgttgtgg ctttgttttt ccttaacgcc
    16801 gtcatttttc ttttcatgtt aatgaaatat ttcactaaca aacaaatttt accaacactc
    16861 attttaagcc ttgcattttt aagtggcctt atctatttag ttgaaaccat tgtaattatc
    16921 cataaaccaa ttaacggcag tacactgatc cagacaaagt cgaatgatgt ttctattttc
    16981 tatattttcc gccaactcag ttttatttgt ttaacctcgc tggcgctctt ttgttatgga
    17041 aaagacaaca tccttgacaa caataagaaa aaaactggca ttctgttgct ggcgctgatc
    17101 ccttttttag tttttcccct tctggcacac aatctgagca gttataacgc tgactattct
    17161 ttgtatgtcg tcgattactg tccggacaat catactgcga cctggggaat caactataca
    17221 aaaatattgg tttgtctgtg ggcattttta ctgttcttta ttatcatgcg tacacgatta
    17281 gccagcgaac tatggccgtt aatagcatta ttatgtctgg catcgctatg ctgcaactta
    17341 cttctactga ctctggatga gtataattat accatctggt atatcagtcg cgggattgaa
    17401 gtctccagta aactgtttgt tgtgtctttt ctgatttata acatttttca ggagctgcaa
    17461 ctctccagca aactggcagt tcatgatgtg ctgaccaata tttataatcg gcgctacttt
    17521 ttcaacagcg tagagtcatt attgtcgcga cctgttgtta aggacttctg tgtcatgctg
    17581 gttgatatta atcagttcaa acgcatcaat gcccaatggg gacatcgtgt gggtgataaa
    17641 gtgctggttt caattgtcga tattatccag caaagcatcc gccccgatga tattttagcg
    17701 cgactggagg gtgaggtgtt tggcttgcta tttaccgaac tcaatagtgc ccaggcaaaa
    17761 atcattgcgg aacgtatgcg taaaaatgtc gaactcctga ccggctttag taacagatat
    17821 gatgttcctg aacaaatgac catcagtatt ggcacagttt tttcaacggg tgacacgcgt
    17881 aatatctcgc ttgtcatgac ggaagcagat aaagccttac gcgaagcgaa aagcgagggg
    17941 ggcaacaaag tgattattca tcatatttaa gtgcaaaaat attcagagcc atgctttttg
    18001 cgtggctttt gcatacaatt tattactatt acccctaaat ttcccctccc cagcggcgca
    18061 gagatgagta taattagcgc ccctgtgcca ggccgcaatc gaactttatc tggttttctc
    18121 gtttcactaa ccgaaggagt gccatttatc atgaaattgc accatagaat gctccggcat
    18181 tttatcgccg caagtgtcat tgtgctgaca tcttccttcc ttatttttga acttgtcgcc
    18241 agcgacagag caatgagtgc ctatctgcgc tatatcgtgc agaaagcaga ctcctccttt
    18301 ctttatgata agtatcagaa tcagagtatt gccgcgcatg tgatgcgcgc tctcgctgct
    18361 gagcagtcgg aagtgtcgcc agaacagcgg cgcgccatct gcgaggcttt tgagtctgcc
    18421 aataataccc atggcttaaa cctgactgcc cataaatacc cgggcttacg cggcacatta
    18481 caaaccgcat ccactgactg cgacacaatt gtggaagctg cagcactatt acccgctttt
    18541 gatcaggcag tggaaggcaa ccgccaccag gatgattacg gttcaggtct tgggatggcc
    18601 gaagagaaat ttcactatta tctcgatctc aatgaccgct atgtctattt ttatgagccg
    18661 gttaatgttg aatactttgc gatgaataac tggtccatcc tgcagtcagg aagtattggc
    18721 atcgatcgca aagatattga aaaggtattt accgggcgta ccgtattgtc gagcatttac
    18781 caggatcagc gtactaaaca gaacgtgatg agtttgctga cgccggtgta tgttgcaggg
    18841 cagctaaaag ggattgtgct gctggatatt aacaaaaaca atctgcggaa tatcttttac
    18901 actcatgacc gccctctcct ctggcgtttt ctcaatgtca cgctaaccga tacagattcg
    18961 gggcgcgaca ttatcatcaa ccagagcgaa gataatctgt tccagtatgt cagttacgtc
    19021 catgacttac cgggcggcat tcgtgtctcg ttatccattg atattcttta ctttatcacg
    19081 tcttcgtgga aaagcgttct gttctggatt ttgacggcgt taattttgct gaatatggtg
    19141 cggatgcact tccgcttata ccaaaatgtg tcgcgagaaa atattagtga tgcgatgact
    19201 ggactgtata atcgcaaaat tttaacccct gaactggagc agcggttgca gaaactggtg
    19261 caatccggtt cttcggtgat gtttattgca attgacatgg acaagttaaa gctaataaat
    19321 gacaccctcg gtcatcagga aggggattta gcgattacgt tgttagctca ggcgattaaa
    19381 caatcgattc gtaaaagtga ttatgccatc cgactcggtg gcgatgaatt ctgcatcatt
    19441 cttgtcgatt cgacgccgca aattgcagca caactgcctg aacgtatcga aaaacgtctg
    19501 caacatatcg cgccgcagaa agagatcggc ttctcttccg gtatttacgc gatgaaagaa
    19561 aacgatacgt tacatgatgc gtataaagct tccgatgagc gtttatatgt caataagcag
    19621 aacaaaaaca gccgttcatg ataaccttct gtggttgttt gcttgtaatc tcaggagcgt
    19681 gaaatgactg aaatggctaa aggaagcgtg acgcatcagc ggttaatcgc tttattatca
    19741 caagaaggtg ctgatttccg cgtcgttacc catgaagcgg taggtaaatg tgaagcggtg
    19801 tcagaaattc gtggcaccgc tctaggccag ggtgctaagg cactggtatg taaagtcaaa
    19861 ggcaatggcg taaatcaaca tgttctggcg atcctcgcag ccgatcaaca ggccgatctg
    19921 agccaactcg ccagccatat aggcggatta cgcgcttcgt tggccagccc ggcggaagtc
    19981 gatgaactca caggctgtgt cttcggcgcg atcccccctt tcagcttcca tccaaaactc
    20041 aaactggttg ccgaccctct actcttcgaa cgatttgatg aaatcgcctt caatgcaggg
    20101 atgctggata aatccgttat tttgaaaacc gcggattatt tgcgcattgc gcaaccagaa
    20161 ctggtcaatt tccgccgcac tgcgtaacta gcctgtccgt tcaataagca gaacggacaa
    20221 aaccagcaca acagcaatgg caaaaaacga tgacgtgata atcagtgtct cgacgaacat
    20281 ttgatcgttc atagcatacc cccgggagat agctgttttc acgttattgt tagcgtgcac
    20341 aaatggcagt ttgatgacag ttcgcctgat tttttatgca aaaaacgtaa agattttcta
    20401 cttccttcct gcagcaagcg taaagtaagc aggcttatta ttttttggca aggaaaccac
    20461 gatgtttgat gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca
    20521 taacaccact gtcgccgttt caattctggt gttgatcatt gtccgcgtca caccgttaag
    20581 cacctttttc ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat
    20641 tggcgttatg gcccccatcg ccagcgggac gctaccgccc tcaaccttga ttcactcgtt
    20701 tctgaactgg aaatcgctgg tggcaattgc agtaggcgtt attgtctcct ggctgggtgg
    20761 gcgcggcgtg acgttaatgg gcagccagcc gcaactggtt gctgggctgc tggtgggcac
    20821 tgttttaggc gtagcgctgt ttcgcggcgt accggttggc ccgcttattg ccacgggtct
    20881 ggtttcgctg attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc
    20941 cccaggcctt tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga
    21001 gcaactttct gtacagtatc gcctttcact aatccctgaa gcgccataat cagctgtaac
    21061 tgctgtcgcc actggcgaaa actcaacccc gtctctttta ctatcagtcg ggcaagattg
    21121 cgttcactca tcgcaaaaaa gccagcccat tgccccaatg ccccccactc gacgggctct
    21181 ttcgccatca tctccaccat cgcgcggatt ttaggatgag aagaaacggg taggtgcagc
    21241 tgttgttgtg gctgctgagg gagttcatca aacaggacct gaattaatct gcgggtcatc
    21301 ggctcagcac gctgagtcgt cgtcctgttt gccagagtta aaattaattc ccggcataag
    21361 ggcgatattt tcaatgtgta acatgttgtc ggcattgtca ctgcgctggg ttcgataaac
    21421 agaaaacaga gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc
    21481 caaaccgcat attgtggcgg caccatccac aaagcatttt ccaccgtaca ggtaattgca
    21541 ccatgtagcg ccagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca
    21601 tccgttccgg cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag
    21661 ccattgagat tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag
    21721 cttgattcaa cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc
    21781 atatgacctg ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc
    21841 tgatgattgc cactacatta cgcgtcacct tcaccggcgc agcaccgtta ctcgatacga
    21901 ttcgttccgc ttactcgctg acgactgcgc aaaccggctt attgaccacc ctgccattat
    21961 tggcctttgc gctaatctca cctctggctg ccccggtggc tcgacgtttt ggtatggaac
    22021 gtagcctgtt tgccgcgtta cttttgatct gtgcaggtat cgcaattcgc tctctccctt
    22081 cgccttactt attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggtaatg
    22141 tcttactgcc aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg
    22201 catattccct gacaatggga gctgcggcgg ctctgggatc ggctatggta gtgccgctgg
    22261 ctttgaacgg ttttggctgg caaggcgcgt tgctcatgct gatgtgtttt cctctgctgg
    22321 ctcttttttt atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacttcac
    22381 gcgccttaca tactcggggt atctggcgtt cgccgcttgc ctggcaggta acattgtttc
    22441 ttgggatcaa ctcactggtc tattacgtga ttattggctg gcttccggcg atcctcatca
    22501 gtcacggcta tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag
    22561 cagctcccgg tttgctgatc ccacttttct tacatcatgt gaaagaccag cgtggtattg
    22621 cagcgttcgt tgccttgatg tgcgcagtgg gcgcggttgg gctctgcttc atgccagcgc
    22681 acgcgatcac ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt
    22741 tgacgttcat tggtctgcgg gctagttctg cgcatcaggc ggcggcactc tcggggatgg
    22801 cacaatccgt cgggtatttg ttggcagcct gtgggccgcc gctgatgggt aaaatacacg
    22861 atgctaacgg tgactggtcc gtaccactaa tgggtgttgc aatactttca ctactgatgg
    22921 cgattttcgg actttgtgcc gggagagaca aagaaattcg ctaatatccg gtactatagt
    22981 gacgtgacaa atcatgcgtg aaagggagaa caaacacgat gaatattcag tgcaaacgcg
    23041 tttatgatcc ggcagaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccac
    23101 gcggtatcaa aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa
    23161 cggaactgcg caaagccttt cacggcgaag tcgtcgattt tgcaaccttt cgcgagcaat
    23221 atcttgcaga actggcacaa cacgagcaag aaggaaagcg gctggcggac atcgccaaaa
    23281 aacagccgct gaccctgctc tactcagcaa aaaacaccac gcagaaccat gcgctggtgc
    23341 tggccgactg gctacgtagc ttgtgatttt agtacagcat ccggcggtta tttttcacca
    23401 gccggatggt ccagtcgcca caatgcccat tcatcaatcg tttcaccgcc cggtaatttg
    23461 caattgttgc tgaccccttg cgctgtctgc actggaatga gcgtcccgcc cttctgctgg
    23521 caatagaccg acgccggatt tgccatacca atctgcggcg gtttaggtgc ttctggctga
    23581 gaaggggttg aacaaccagc caggaccagc aagcaaggca gaacaaaact gataattttc
    23641 atttattgat ctcacatatg tatccaagct tagagtatcg cggtatcgtt ttgttttgca
    23701 gcactatttt tattacattc actcaaaaca tattacgtct tgtttcatct ttgttgatga
    23761 tgttttatca tgcctgcaaa gattaaataa tcagcattta cccgccgtat cctggagttg
    23821 tttcgtgtca gatcagatta tcgcccgcat ctcgcaatcc cttgccaaag aacagtcact
    23881 ggaaagtctg gtccgacagc ttctggagat gctggaaatg gtcactgata tggaatcaac
    23941 ctacctgacc aaagtggatg tcgaagcgcg cctgcagcat attatgtttg cccgtaacag
    24001 ccagaaaatg cacatcccgg agaattttac cgtctcgtgg gattactcgt tatgcaaacg
    24061 cgccattgat gaaaactgct ttttcagcga tgaagtccct gaccgttggg gcgactgtat
    24121 tgcggcacgc aatcttggca tcaccacatt tctgagcacg ccaattcact taccggatgg
    24181 atcattctat ggcacgcttt gcgccgccag cagtgagaag cgccagtgga gtgaacgcgc
    24241 ggaacaggtt ttacagttat tcgccggact gattgcacaa tatattcaaa aagaggcact
    24301 ggttgaacag ctgcgcgaag ccaatgctgc gctgattgcg caatcgtata ccgactcgtt
    24361 aaccgggcta ccgaatcggc gggcgatttt tgaaaatctg acgacgctgt tttccctcgc
    24421 ccggcatctt aaccaaaaga taatgatcgc gtttatcgat ctggataact tcaaattaat
    24481 caatgatcgt tttggtcata atagtggcga tctgtttctc attcaggttg gcgagcgcct
    24541 taatacgctc cagcaaaatg gcgaagttat tggtcgtctc ggcggtgatg agtttttggt
    24601 tgtttcacta aacaacgaga atgcggatat ttcgtcgctg cgagaacgca ttcagcagca
    24661 aatacgcgga taatatcact taggtgatgt tgatttgtat tatcctggtg ccagtcttgg
    24721 catagtagaa gtcgatcctg aaacgaccga tgcagacagt gccctacatg ctgccgatat
    24781 cgcgatgtat caggagaaaa aacacaaaca gaaaacacct tttgtcgcgc attcagcgcc
    24841 acattcctga ggcgtattca catccttttg attggtgata acatgcgaat cggtattatt
    24901 tttccggttg taatcttcat tacagcggtc gtatttttag catggttttt tattggcggc
    24961 tatgctgccc cgggagcata aagatgaaaa aaacaacgat cattatgatg ggtgtggcga
    25021 ttattgtcgt actcggcact gagctgggat ggtggtaacg tcacatctaa aaaatagcaa
    25081 aggctgcctg tgtgcagcct ttgtgcaatt tacgcgttaa cttttaatct tcctgtagat
    25141 aaatagcacg acaatcgcac caataacggc aaccacgaag ctgccaaaat tgaagccatc
    25201 gactttacca aagccaaaca gcgtgctgat ccaaccgccg actacggcac cgactatccc
    25261 cagcaggata gtcataaaga atccaccacc atctttacct ggcatgatcc acttcgccag
    25321 aataccggca ataagcccaa aaataatcca tgacagaatg cccattgttt cctcacttat
    25381 ctgttttgca ttagcgggtt agtcgctgat aaaaagcata gcacaacatc gggagggcaa
    25441 gatttgtgac gagcatcacg gaggtttttt tgcgatggcg cagaaattgc gccatcaacg
    25501 atcagtgata attaccaacc acaaacatca tgttcgtttt ccgtgtcata agaacgcacg
    25561 gtattcacca gatcttttat cacttcagcc gccacttctg gcaccagcaa agtcatcggc
    25621 gtctctgttt cataatcgac agaaacgcca ttgctgttat tggtgacggt cacggtatac
    25681 gttgctttgc ccatgattca tttcccgtta tgaatgactt tccgttgttg cgcaccttcc
    25741 atcaggactt caggagccac gaagaagtca atgttgaaat aagtatcgtc agtcatggct
    25801 tcaatgttgt gccacttttc tggagggaac accgcaaact gccccgcttc gataaggatc
    25861 acctgatcag gctctgcact gtgttcatca gcgtagccga gatatttgac cgccccatgc
    25921 ataacggaaa ggcgtgggta aacccccggg cgcgttcctt tatcaagatg acgttcgaat
    25981 attccggcag gcgcagtttg tttattccag aaaggcgttg agcgcgtatg aatataattc
    26041 tgtgggattt gcagcatcct tttccctcct tcggtgaatg cgctgaaaac ggtttattcc
    26101 agccgtttca gggtacgcct gataatttgc tttttaaata ccatttattg gttacttttt
    26161 agcaccatat cagagaaaaa tcagggagga ttatagatgg gaagcccgcg acaacgcggg
    26221 ctgaaagcat caggattgca gcgtcgccag tcgggcagcg aaacccacga acatcaaacc
    26281 aatcagtgag ttgcccactt tagccagttt ctttttggta cgtatgtact gcgtgacaaa
    26341 agcaccagat ataatcagga agctcaaata gcagaaactc accagttcca gcgtcgtcgc
    26401 cagaataaag aatgaaattc ccgtatgtgg ggcattaaca tcgataaact gtacgaaaaa
    26461 cgacacatag aacaaaatgg ctttcggatt agtcaggctc aaaattaacg cgcgtttaaa
    26521 aatagcaccg tattggggct catcggattt ggcctcgtta tttttaccct tcagggtcgc
    26581 gtaaagaatt ttactcccca gatagagcaa ataaaacgca ccaagataac gtacaatgtt
    26641 gaataatatc ggggtggtct taattaatgt cgccattcca gcccatgcca gaaacatcaa
    26701 taccgcatcg ccaataaata caccgcaggc cgcaagataa ccgcctttca taccgctact
    26761 gacgctattt ttgagtacaa acagggtatt tggccctggc accaacacaa taaaaatggc
    26821 cccaaccaga taggtccagt aattcagaac cccgtattca gcgaacacat taacctcttt
    26881 aattatcttt cgatcatgcg cgattaaagg tgaatatgct aaccaatctg tagcggctta
    26941 gaaaggagaa aatcaggttt taacctgata tcaacccgat aattgaatca ttaatcaggc
    27001 atgctccagt gaaaaattcg ggtagtgctc ggcaaaatac tggcgtaaaa attctaccgt
    27061 tatccgcact ttcgctgacg tcgccagcct tgaaacataa acggcccaga cgttcgctgg
    27121 ctggtaatat tccggtagca cttgcactaa atgaccacta gcaatgtttt cgctaacatc
    27181 ccaccaggaa cgcagagcaa tcccctgccc atcgagacac cactgatgga caatttctcc
    27241 atgattagac gacagtgggc cggtgacttt aatcgcatgg tggccttctt tattgcgtaa
    27301 ttgccagaca ccaaaaggat gatcgcgctc tttaatgacc aggcacggca atgcagaaag
    27361 atcggttaaa tgtttcggcg cgccatgttg cgcaataaat tccggcgacg cgcagagaat
    27421 gcggtaattg gtcgccagtt tgcgggcgat taaattaggg gcgatgtcat cgccaatgcg
    27481 aatatcgaga tcgacacctt cattgaccaa atcgaccagc cggtcttcca catcaaaacg
    27541 taattccagt tgcggatacg ccttcgccag cgctgataac gccggagcca ccacctgtcg
    27601 tccaaaacca aagctgctga taatacgcag catcccctgt ggcacctgac gcacgtcaga
    27661 aagttcgtcc atcatctgac cgacatcctg caaaatccgc tgcgcccatt cataaatccg
    27721 ctctccttct tcggtaatgg tgacgcggcg ggtggtgcgg tgtaacaaca ccacgtttag
    27781 cgtttgctcc agcaaggcga tgcgcttgct gacgaacgcc ggtgaaacgc ccagttcttc
    27841 cgccacggcg gcaaaaccag cccggcgagc caccagcata aagacgcgca aatcattcag
    27901 cagcggtaaa ttattcatga ttcgtgtttt atgtttcacc agttacgggg attaattcct
    27961 tttcagtcaa ttataggatg gtgatgttgt caattttgat ggtcaggaag tgagaaccca
    28021 atgatgaaaa cgatgcgtat tgctgcgatc ccgggagacg ggattggcaa agaagtcctt
    28081 cctgaaggga ttcgcgtgtt acaggctgcc gctgagcgct ggggcttcgc cttgagtttt
    28141 gagcaaatgg agtgggcgag ctgcgagtat tacagccatc acggtaaaat gatgccggac
    28201 gactggcatg agcaacttag ccgtttcgac gccatctatt ttggtgccgt cggctggccg
    28261 gacaccgttc cggaccatat ttcgttgtgg ggttcgctgc tgaaatttcg tcgtgaattc
    28321 gaccagtacg tcaacctgcg cccggttcgt ctctttcctg gcgttccctg cccgctggcg
    28381 ggaaaacagc ctggcgacat cgatttttac gtggtcaggg aaaacaccga aggcgaatat
    28441 tcctcgctcg gcggtagagt gaatgaaggt acagagcatg aagtcgtcat tcaggaatcg
    28501 gtatttacgc gtcgtggtgt cgatcgcatt ttgcgttatg ccttcgaact tgcgcaaagc
    28561 cgcccacgta agacgctaac ttctgccact aaatcaaacg gtttagccat cagcatgccg
    28621 tactgggatg agcgagtgga agcaatggcc gagaattacc cggagatccg ctgggacaag
    28681 cagcatattg atattctctg cgcgcgtttt gtgatgcagc cggaacgctt cgatgtggtg
    28741 gtggcgtcca atttgtttgg cgatatcctt tccgatcttg gcccggcctg caccggcacc
    28801 attggcattg ccccatccgc caacctgaat ccggaacgca ctttcccctc gctcttcgag
    28861 cctgtccacg gttccgcgcc ggatatctac gggaaaaata ttgctaaccc tatcgccaca
    28921 atttgggccg gggcaatgat gctcgatttt ctcggcaatg gcgatgagcg tttccagcaa
    28981 gcgcataacg gtattctggc agcgattgaa gaagtgattg ctcacgggcc gaaaacaccg
    29041 gatatgaaag gcagtgccac cacgccacag gttgccgacg cgatttgcaa aattattttg
    29101 cgttaaggtc aaaccagttt atttgaaccg cgtcactgac gcggttttgt tattcgttct
    29161 ttgcagttaa taacctgcgt catttcacct tttattgttt ccgtttcgtg ttttatggct
    29221 ttccgtattc ttaattgttt aatttatgta acatgcaaat aatcaattcg ttaaacacga
    29281 catacaactc attaccctgc aaataattac aactggcaaa agccagagac gggcttttat
    29341 gcccaggaga tgactcatga tgagcaatgt taagaaaaaa gatgtgccgc tgataagcat
    29401 cagcctggtg gccattcttt tcatcgcagc tgcattaagc cttttcccac aacaatcggc
    29461 cgacgcggcc aacgcaatat acacttttgt tacgcgtacg ttaggttccg cagtacaggt
    29521 attggttttg ctggcaatgg gactagtgat ttatttagcc accagtaaat acggcaatat
    29581 tcgtcttggc gaaggaaaac cggaatacag cacgctctcc tggctgttta tgtttatttg
    29641 tgccggttta ggttcttcta cgctttattg gggggttgct gaatgggcct attattatca
    29701 aacgcctgga ttaaatatcg caccgcgttc acaacaggca ctcgaattta gcgtccctta
    29761 ctccttcttc cactggggca tcagcgcctg ggcaacttat acgctggcct cattaatcat
    29821 ggcttatcac tttcatgtgc ggaaaaacaa aggtctgagt ctttccggca ttattgccgc
    29881 gattaccggc gttcgcccgc aaggcccatg gggaaaactg gttgatttga tgttcctgat
    29941 cgccactgtc ggcgcactga ctatttccct tgttgtcacc gcagcaacct ttactcgtgg
    30001 actttccgca ctgaccggtt tacccgataa cttcaccgtg caggcatttg tgatcctgct
    30061 ttccggcggc attttttgcc taagctcatg gattggtatc aacaacggtt tgcaacgtct
    30121 gagcaaaatg gttggctggg gcgcgttcct gctgccatta ctggtgctga ttgtcggtcc
    30181 aaccgaattt attaccaaca gcatcatcaa tgccatcggc ctgaccacgc aaaacttcct
    30241 gcaaatgagc ttattcaccg atccgcttgg cgatggttca tttacccgca actggaccgt
    30301 tttctactgg ctgtggtgga tctcatacac ccctggcgta gcaatgtttg tcacccgcgt
    30361 ttcccgcggt cgtaagatta aagaagttat ctggggactg atcctcggca gcaccgtcgg
    30421 ttgctggttc ttcttcggcg taatggaaag ctatgccatt catcagttta tcaatggcgt
    30481 aatcaacgtc ccacaggtgc tggaaactct gggcggcgaa acagctgtac agcaagttct
    30541 gatgtcgttg ccagccggta aattgttcct cgccgcatac ctgggcgtga tgattatttt
    30601 ccttgcctcg catatggatg cagtggccta caccatggcg gcgaccagta cgcgtaatct
    30661 ccaggaaggt gacgatcctg accgtgggct gcgtcttttc tggtgcgtgg tgatcactct
    30721 gatcccgctt tccatcttgt ttaccggtgc ttcgctggaa acgatgaaaa ccaccgtcgt
    30781 gctcacagcc cttcccttcc tcgtcatttt actggtgaaa gtcggcggat ttattcgctg
    30841 gctgaaacag gattacgccg acattccggc tcatcaagtt gaacattatc tcccgcagac
    30901 accggttgaa gccctggaaa aaacaccagt gctccctgcg ggaaccgtat tcaaaggcga
    30961 caactgagcg ccatcatcct aacgataaag gtatccctat gagcaatctg agccctgact
    31021 ttgtactacc cgaaaatttt tgcgctaacc cgcaagaggc gtggaccatt cctgcccgtt
    31081 tttataccga tcagaacgcc tttgaacacg aaaaagagaa cgtcttcgcc aaaagctgga
    31141 tttgcgtcgc tcacagcagc gaactggcga atgccaatga ttatgtgacg cgtgagatca
    31201 ttggcgaaag catcgtgctg gtacgcggtc gtgataaggt tttgcgcgcg ttctataacg
    31261 tgtgtccgca ccgtggtcat cagttgttga gcggtgaagg aaaagcaaaa aatgtgatta
    31321 cctgcccata tcacgcatgg gcattcaaac tcgatggcaa tctggcccat gcacgtaact
    31381 gcgaaaacgt cgccaatttc gatagcgaca aagcgcaact ggttccggtg cgtctggaag
    31441 aatatgccgg attcgtcttc atcaacatgg accccaacgc caccagcgta gaagatcagt
    31501 tacccggtct gggcgcgaaa gtgctggaag cctgcccgga agtccacgat ctgaaactgg
    31561 cggcccgctt taccacccgc acgcctgcca actggaagaa cattgtcgat aactatctcg
    31621 agtgctatca ctgtggtccg gcgcatccag gtttctccga ctccgtacag gttgatcgtt
    31681 actggcacac catgcacggt aactggacgc tgcaatacgg tttcgccaaa ccgtccgaac
    31741 agtcgtttaa atttgaagag ggtacggatg cggcattcca cggtttctgg ctgtggccgt
    31801 gcacgatgct gaatgtcacc ccgatcaaag ggatgatgac ggtcatttat gaattcccgg
    31861 tggattctga aactaccctg caaaactacg atatttactt caccaatgaa gagttaaccg
    31921 acgagcaaaa atcgctgatt gagtggtatc gcgatgtgtt ccgtccggaa gatttacgtc
    31981 tggttgaaag cgtacagaaa gggctgaaat cgcgtggcta tcgtggtcag gggcgcatca
    32041 tggccgacag tagcggtagc ggcatttccg aacatggtat cgcccatttc cataatctgc
    32101 tggcgcaggt atttaaggac taatgacatc ggcggcggta ttttccgccg ccgggctgat
    32161 ttttgatgga gtacagcaat gtcagactat caaatgtttg aagtacaggt gagtcaggtg
    32221 gaaccgctta ccgaacaggt gaaacgcttc acgccggtgg caaccgatgg caaaccgctg
    32281 cctgcgttca ccggaggaag tcacatcata gtgcagatga gcgatggtga taaccagtac
    32341 agtaatgcgt attcactact gagttcaccg cataacacct ctagttatca gattgccgtt
    32401 cgactggaag aaaactcgcg cggcggttcc cgctttttgc atcagcaggt aaaagtgggc
    32461 gatcggttaa cgatctcaac gcctaataac ctgtttgcgc taattccctc agccagaaag
    32521 catctgttta tcgcgggcgg tattggtatc acccctttcc tgtcgcacat ggcagagttg
    32581 caacacagcg acgtcgactg gcagctacat tactgctcgc gaaatccaga aagttgcgca
    32641 tttcgtgatg agctagtcca gcatccgcag gctgagaaag tccatttgca tcaatcatcg
    32701 accggaacac gactggaatt agcgcgatta ttggcggata tcgaacctgg cacacacgtt
    32761 tatacctgtg gccccgaggc gctaattgaa gcggtaagaa gtgaagctgc gcgtctggac
    32821 atcgctgccg atacgctgca ctttgagcaa tttgctatcg aagacaaaac cggcgatgca
    32881 tttaccctgg tgcttgcccg ttccggaaaa gagtttgtgg tgccggaaga gatgactatt
    32941 ttgcaggtta ttgaaaataa taaagccgcg aaagtggaat gtttatgtcg tgaaggggta
    33001 tgcggaacct gcgaaacggc aatactggaa ggtgaagctg accatcggga tcagtatttt
    33061 agcgatgaag agcgtgccag ccagcaaagt atgttgatct gttgttcgcg tgcgaagggt
    33121 aaacgcctgg tgttggattt gtagtttgcc gaagccggat gtggcgctga gcgcgcccag
    33181 tccggcttcg gaaaatttta ctgcggatat tcctgcaata aattgtgtaa tgcttccgcc
    33241 atcagctcac cacgccagcc ggaaatcagc tccggcaaat tgttctgcgg tttcagtttc
    33301 cagtgccagt tcagcagttg gttgatttgc cgacgcgatg ccagcaattc ggcgctgatc
    33361 ttgtgcgttt cgctcacgtc agtaatcagc gacttaatcg ctttaaacgc tttacgataa
    33421 cccggcatgt ccatcaggtt aagcatcggc tgcggtaagg cctcttccgg caatgtctgc
    33481 gctttttcca ccagcgccag cagcgtttta ccgtgaaagc ggatttcgct accggataaa
    33541 cccaggctgt ccagttcgcc taaacttccc ggcatataac gcgctaccga ccacaaatgc
    33601 tcttcacgca cgacaaagtt caccgccaga tcgcgctctc gcgccttgcg cagtcgccag
    33661 tcggctaaca gttgcagaca ggccagttgg cgtgtgcgta attgccaggc attggtgata
    33721 tcacgccagg catcttccgg cgcaacgact tcctgacgac gcatttgcat caggcgacat
    33781 tcatccagcg ccgctggtag ccagccggag gcctccgttt ctaccataag cttggcggtg
    33841 atcggtaaca gataccagac atccgccgct gcatattcac actgacgttc ggtcagcggt
    33901 ctggccagcc agtcggtgcg cgattcactc ttgtccagcg taacgccgga atactcttcc
    33961 accatggaag cgaaacccca tgacatcggg cgtccgcaga aggcagcaag gatttgcgtg
    34021 tcaatcaggg gttgtggtaa ttcgccaaag acattgagga acacttccag atcttcactg
    34081 cctgcatgga gaaattttgt gatggacgga tcgcgcagga tcgctttcag cggtgaccag
    34141 tcggtgatcc cgagaggatc aattagcgcc agatgctcgc catcgaaaag ttgaatcaac
    34201 cccagctgcg ggtaataagt gcgcgtacga acaaattcag tatccagggc tatcgccgga
    34261 aaggcacgga cggcttcaca caaagaagcc agcgcatcgt ccgtggtaat catttggtaa
    34321 ttcaaattgt tttctcttta gtgggcgtca aaaaaaacgc cggattaacc ggcgtctgac
    34381 gactgactta acgctcaggc tttattgtcc actttgccgc gcgcttcgtc acgtaattct
    34441 cgtcgcaaaa tttttccgac gttagatttc ggtaactcat cacgaaactc caccagcttc
    34501 ggtactttgt agcccgtgag ctgacggcgg caaaaggtca ccagtgactc ttcggtaagc
    34561 gatggatctt ttttcactac gaagattttc accgcttcac cactggagcc ggaaggtaca
    34621 ccaacagccg cgacttcctg tacgccagga tgctgcatga cgacatcttc aatctcgttg
    34681 ggatagacgt taaaaccgga aaccaaaatc atgtcttttt tacgatcgac aatgcgcagg
    34741 aatccttctt catccattac cgcgatgtcg ccagtgtgta accagccgtt tttgatgatt
    34801 tcatcggtag catccggacg ctgccaatag cccagcatca cctgtggacc tctgacgcaa
    34861 agttcacctg gctgacctgg cgatacttca ttatcgtcgt catccaccag tttggcttcc
    34921 gtcgacggca ccggcaaacc aatgctaccg ctatgataat caatatcata ggggttcacg
    34981 ctgaccagcg gcgcacattc ggtcaggcca taaccttcca gcagatactg tccggtcagt
    35041 ttcacccagc gttctgccac cacttgctga acaggcatcc caccgcctgc agaaaggtgc
    35101 agactggaga aatccagttg ttgaaactct ttattgttca gcaacgcgtt gaacaaggtg
    35161 tttacgccgg tgatagcggt aaacggatat ttcgccagct ctttgaccag ccccggaata
    35221 tcccgcgggt tagtgataag caggttctgc ccacccagtt cgataaacag caggcagtta
    35281 atggtcagtg caaaaatgtg atacagcggc agcgccgtca ccaccagctc tttgcccgga
    35341 tgcagcagag gcccatacgt agcgttaact tgttccaggt tcgccagcat attgcggtga
    35401 gtcagcatcg cgcctttcgc cacacctgtg gtgccgccag tgtattgcag gaaggctaaa
    35461 tcttctggca ccagttcagg tttgacatac tgcatacggt agccgttgtg cagggcgcta
    35521 cgaaatgaaa tggcatctgg cagatgatat ttcggcacca gacgtttgat gtatttaaca
    35581 acgaagttaa ccagtgtgcc tttcgctgtg gaaagctgat cgcccatacg ggtcaggata
    35641 acgtgctgaa ctgcggtttt atcgaccact ttttccagcg tgtgagcaaa gttggacaca
    35701 atgacaatcg ccgatgcgcc gctatcgtta agctgatgct caagctcacg cggggtatac
    35761 aacgggttaa cgtttacgac gatcatcccg gcacgcaaaa tgccaaacag cgccaccgga
    35821 tattgcaata aattaggcat catcaacgca acgcgatcgc ctttcttcag ccccaaccct
    35881 tgttgcaaat aagcggcaaa cgcgcgactg cgttcttcca gcttgcggaa ggtcatcacc
    35941 tcccccatat tcacaaacgc aggctgatcg gcgtagcgcg cgaccgactg ctcaaacata
    36001 tctaccagag attgataacg gtcagggttg atctccgtcg gaacgtccgc gggataacgg
    36061 ttaagccaaa ccttcttcaa ttcttcacct ctaaaatgcg tgttcgtcgt catcgcaacc
    36121 ccaaatgata tacatgccgt taacataata ttaactcatc ataccagctt gataattacc
    36181 caacgaaaag gttgcgaagc gcgtcactat ttatttttat ctttaccgta agaatgcaga
    36241 aacagcggac cagccgctgt ttctttttct ttaaaaacaa gcaattattc agttacaact
    36301 gtttgtactc tcgcggggcc gggattatac cagccccatc cgccgtggcc atagggccag
    36361 ccacgaccgc cataaaacca gggatcaatc ggctgaggcg gcataatcac ctgctgggtt
    36421 aaatgccaac gtttgtaacc tgttgcctgc atcaccataa atttataggg cgtgttgccg
    36481 atttttccgt caaccgcacc agtgattggc ccgactaccg taaccagttg tccacggaaa
    36541 tccaccgggt ccagaaaacc gttcacatcg gcataaatgc gaccgcgaga aggttctccc
    36601 agcgtcggtc tggctccgct gtccagcggt acggtagcaa tttccaggcg ggttttccct
    36661 tgctggtttt gtaccgcaac cactttgcca ccaaagcgtg cctcctgacc aacgtacagc
    36721 tgcggcgcac tcatcacccg aactaaatct tgttgcggcg tgggactgct gcctttaatg
    36781 gcgtccggca cagtgacaca accgctcagc attagcgcaa acgtacctgc cagtatgcct
    36841 ttgataacat tcttttgaac cgccatgatg cgactccttt ttttctcagg gcatactctt
    36901 aagattcatt ctttgcccgg aagtttcttc catgcgacgt tgttacgtaa ataaaccggt
    36961 tcggcatgtt ccaccgccac cgttttaccc tcggcaaaca tctgacaagc aatcggcagc
    37021 atatcttctg cagcgggcag taaaacttct ccgtcgcgca aaaccagtcc gctctcttta
    37081 ccgagatccg gccaggcttg ccagcctgta cctaccgtca cccattcccc ggaaagctgt
    37141 tgcattcgtt catgaacgag ttcgggtttg agtacggctt cggtttcttc accgtgccag
    37201 ataccgtttt catcacgctg atattcggcc cagtaaactt cgcccattcg cgcgtcaatg
    37261 gctgacagca cgcgtgttgc gccgtttttg cgccacgccc cttgcgccat cgtcattagc
    37321 gtggagacgc caatcatcgg taattccgcg ccaagcgcca gcccttgcgc gatgccaata
    37381 ccaatgcgca cgcctgtaaa gctaccgggg ccgcgcccgt aagccagagc gttaatatca
    37441 gtcagggaag ttccgctggt ggtcaggata tcctgcacca tcggtaagat tcgttgagta
    37501 tgttcacgag ggcaaagctc aaaatgagcg ttgacagtac cgtcgttcca cagggcgaca
    37561 gagcaggcct ctgtcgcggt atcgatagcc agaattcgca tgggtcttcg tgcttagatc
    37621 aataaaaagg cgcgcatcat accatactcc gtaacaaatt acctggagga tggtatcgca
    37681 aggaaacgaa ccgcacgggc aatgtcacgg gtgcgtggcg cgggcggcag actggcgaga
    37741 aacgtcgcgc cgtaaggacg catcaccagc cgattgtcac aaattaccaa aaccccgcga
    37801 tcgtcggcgt cgcgaatcag tcgcccgact ccctgcttga gagtaatcac cgcatccggc
    37861 agttgtactt catcgaacgg gtcgccaccg cgcaaacggc aatcttccat gcgcgctttt
    37921 aacaatggat catcaggcga ggtaaacggc aatttgtcga taatcaccaa tgacaatgta
    37981 tcgccacgca cgtccacccc ctcccagaaa ctactggttg ccacaagaag cgcgttaccg
    38041 gcactgacaa attgctgcaa cagttgccct ttgctggttt ccccctgcaa taatacggga
    38101 agcgtcatgg tagcgcggaa ctgctcggcc agatcgcgca tcatggcgtg cgaggtacaa
    38161 agcataaaac agcgaccgtt gttagcttcg atgatcgggc gcagcatagc cgccagttgc
    38221 cgcgcggaac ccggttgatt ggtttgcggc aggttgcgcg gcacacagag taacgcctgg
    38281 cggctgtaat caaacgggct tggcaatagc aacgactccg cctgttctat cccaagccgc
    38341 gaggtgaaat gatgcagatc gtcgtttacc gacagcgttg ctgaggtgaa gatccagcta
    38401 ccgggctttt gcgccattaa ctctttgaat ttatccgcca cgctgagcgg cgtgagagcc
    38461 agagtaaaat ggcgcgaagt gcattcgtac cagtagctgt agcccggctg attgatctct
    38521 tttagccgct tcagccgtgt gcgatacaac gtggcgcgct caaatgccgc atccagcaag
    38581 gcggaacgcc ccagtgacag tttcgccacg tcataacaaa gttccagggt gtcatcgagc
    38641 agtaaaaatg cccgctgaat ttgcggatta gctaacagct cacgcaggtt accgcgataa
    38701 cctggttcac cgagttgcag acgaaaatcc tgcgcactct gggcaagacg atcagcgcac
    38761 ttttgtaact gctgggtgtc ttttaattcg gtgcggtagg cgatggtgat gtcttttgcc
    38821 aggtcgagca gttgtcgact ggagagtgac tgaccaaaat actggctggc aatgtccggt
    38881 agctggtggg cttcgtcgaa gatcatgacg tccgcttccg ggatcaactc gccaaatcca
    38941 ctctctttaa ccaccatatc cgccagaaag agatgatggt ttaccaccac cacatcggcg
    39001 tccatcgctt ttttacgtgc tttgaccaca aagcaatctt tatacatcgg gcagtcgctg
    39061 ccaagacagt tgtcgttggt gctggtgacc agcggccacg cctgcgaatc ttccgccacg
    39121 ctgacgcagg tgctgatatc accatcgact gtttgattag accaggagcg cagcaggatc
    39181 acatcgctta atatttgtac cggaagatcg ccccccgcca gcgcctgctg ttcgagacgt
    39241 tcgaggcaga ggtagtttga gcgccctttc agcaacgcca cgttgcccgt gtatttcaac
    39301 gcctttgaga ccgtcggcaa atcgcggcta tagagctgat cctgcaacgc ttttgagccg
    39361 gtcgagataa tgactttctt tttcgcccgc agcgcagggg ccaggtaagc gtaggttttg
    39421 cccgtaccgg ttcctgcttc caccaccagc ggctggcctt tttctatcgc ctgggtgacg
    39481 gctaccgcca tctgtcgctg tggttctcgc ggcttaaagc ctggtatcgc tttcgccagc
    39541 tgaccgtctg gtgcaaaatc gtccgtcaca ctaccccctg ttgatttgaa cagggattat
    39601 gtcaggatga gggcgctttc gccagttgaa gtggtgacgg cgacctcaca ttgtggcagt
    39661 ctttgcagca cgaaaatgga aggataatga ggataaaatg atgactatcg ttcgtatcga
    39721 tgctgaagcc cgctggtctg atgtagtaat ccacaacaac acgctctact acactggtgt
    39781 accggaaaac ctcgacgccg atgcctttga gcaaaccgcc aacaccctgg cgcagattga
    39841 cgccgtgctg gaaaaacagg gcagcaataa atcgagcatt ctggatgcca ccattttcct
    39901 ggccgataaa aacgacttcg cggcgatgaa taaagcgtgg gatgcctggg ttgtcgcggg
    39961 tcatgcgccg gtgcgctgca cggtacaagc gggtttgatg aacccgaagt ataaagttga
    40021 aattaagatt gtggctgcgg tgtaagcttt atcgaagcaa aataagtcag acgataattt
    40081 atcgataata ctggtcggtt atacataaat cgaccaga
//
LOCUS       DAZFDC010000025        39613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFDC010000025 DAZFDC010000000
VERSION     DAZFDC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.025"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            633..1892
                     /gene="codB"
                     /locus_tag="ACHJ48_001495"
     CDS             633..1892
                     /gene="codB"
                     /locus_tag="ACHJ48_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO4707081.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSLIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1882..3165
                     /gene="codA"
                     /locus_tag="ACHJ48_001496"
     CDS             1882..3165
                     /gene="codA"
                     /locus_tag="ACHJ48_001496"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO4707082.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKLEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYSIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3298..4197)
                     /gene="cynR"
                     /locus_tag="ACHJ48_001497"
     CDS             complement(3298..4197)
                     /gene="cynR"
                     /locus_tag="ACHJ48_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO4707083.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIHLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHLPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAIPLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            4307..4966
                     /gene="cynT"
                     /locus_tag="ACHJ48_001498"
     CDS             4307..4966
                     /gene="cynT"
                     /locus_tag="ACHJ48_001498"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO4707084.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4997..5467
                     /gene="cynS"
                     /locus_tag="ACHJ48_001499"
     CDS             4997..5467
                     /gene="cynS"
                     /locus_tag="ACHJ48_001499"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO4707085.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5473..6654
                     /gene="cynX"
                     /locus_tag="ACHJ48_001500"
     CDS             5473..6654
                     /gene="cynX"
                     /locus_tag="ACHJ48_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO4707086.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTIPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(6757..7368)
                     /gene="lacA"
                     /locus_tag="ACHJ48_001501"
     CDS             complement(6757..7368)
                     /gene="lacA"
                     /locus_tag="ACHJ48_001501"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO4707087.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRRKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(7434..8687)
                     /gene="lacY"
                     /locus_tag="ACHJ48_001502"
     CDS             complement(7434..8687)
                     /gene="lacY"
                     /locus_tag="ACHJ48_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO4707088.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(8739..11813)
                     /gene="lacZ"
                     /locus_tag="ACHJ48_001503"
     CDS             complement(8739..11813)
                     /gene="lacZ"
                     /locus_tag="ACHJ48_001503"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO4707089.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATLFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(11936..13018)
                     /gene="lacI"
                     /locus_tag="ACHJ48_001504"
     CDS             complement(11936..13018)
                     /gene="lacI"
                     /locus_tag="ACHJ48_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO4707090.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPITSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(13055..14008)
                     /locus_tag="ACHJ48_001505"
     CDS             complement(13055..14008)
                     /locus_tag="ACHJ48_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFO4707091.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKRFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(14028..14804)
                     /locus_tag="ACHJ48_001506"
     CDS             complement(14028..14804)
                     /locus_tag="ACHJ48_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO4707092.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEATMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIIA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(14919..15752)
                     /gene="mhpR"
                     /locus_tag="ACHJ48_001507"
     CDS             complement(14919..15752)
                     /gene="mhpR"
                     /locus_tag="ACHJ48_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO4707093.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15943..17607
                     /gene="mhpA"
                     /locus_tag="ACHJ48_001508"
     CDS             15943..17607
                     /gene="mhpA"
                     /locus_tag="ACHJ48_001508"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO4707094.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     LREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            17609..18553
                     /gene="mhpB"
                     /locus_tag="ACHJ48_001509"
     CDS             17609..18553
                     /gene="mhpB"
                     /locus_tag="ACHJ48_001509"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO4707095.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            18571..19437
                     /gene="mhpC"
                     /locus_tag="ACHJ48_001510"
     CDS             18571..19437
                     /gene="mhpC"
                     /locus_tag="ACHJ48_001510"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO4707096.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            19447..20256
                     /gene="mhpD"
                     /locus_tag="ACHJ48_001511"
     CDS             19447..20256
                     /gene="mhpD"
                     /locus_tag="ACHJ48_001511"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO4707097.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            20253..21203
                     /gene="mhpF"
                     /locus_tag="ACHJ48_001512"
     CDS             20253..21203
                     /gene="mhpF"
                     /locus_tag="ACHJ48_001512"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO4707098.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHDQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            21200..22213
                     /gene="mhpE"
                     /locus_tag="ACHJ48_001513"
     CDS             21200..22213
                     /gene="mhpE"
                     /locus_tag="ACHJ48_001513"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO4707099.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVDTHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAVDKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            22389..23600
                     /gene="mhpT"
                     /locus_tag="ACHJ48_001514"
     CDS             22389..23600
                     /gene="mhpT"
                     /locus_tag="ACHJ48_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO4707100.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRLRMQPCADA"
     gene            23702..24241
                     /gene="yaiL"
                     /locus_tag="ACHJ48_001515"
     CDS             23702..24241
                     /gene="yaiL"
                     /locus_tag="ACHJ48_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO4707101.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(24366..25199)
                     /gene="fghA"
                     /locus_tag="ACHJ48_001516"
     CDS             complement(24366..25199)
                     /gene="fghA"
                     /locus_tag="ACHJ48_001516"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO4707102.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMNHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYHPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(25292..26401)
                     /gene="frmA"
                     /locus_tag="ACHJ48_001517"
     CDS             complement(25292..26401)
                     /gene="frmA"
                     /locus_tag="ACHJ48_001517"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO4707103.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(26436..26711)
                     /gene="frmR"
                     /locus_tag="ACHJ48_001518"
     CDS             complement(26436..26711)
                     /gene="frmR"
                     /locus_tag="ACHJ48_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO4707104.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(26871..27917)
                     /gene="fbpC"
                     /locus_tag="ACHJ48_001519"
     CDS             complement(26871..27917)
                     /gene="fbpC"
                     /locus_tag="ACHJ48_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFO4707105.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARTLILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPVQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADAA"
     gene            complement(27929..30007)
                     /locus_tag="ACHJ48_001520"
     CDS             complement(27929..30007)
                     /locus_tag="ACHJ48_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4707106.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFRQATREQRSRHYFDAGWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGELAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCAFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(30076..31107)
                     /locus_tag="ACHJ48_001521"
     CDS             complement(30076..31107)
                     /locus_tag="ACHJ48_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4707107.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(31104..32420)
                     /gene="uhpC"
                     /locus_tag="ACHJ48_001522"
     CDS             complement(31104..32420)
                     /gene="uhpC"
                     /locus_tag="ACHJ48_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4707108.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(32493..34034)
                     /locus_tag="ACHJ48_001523"
     CDS             complement(32493..34034)
                     /locus_tag="ACHJ48_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO4707109.1"
                     /translation="MSRNLRHWVILLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(34034..34663)
                     /locus_tag="ACHJ48_001524"
     CDS             complement(34034..34663)
                     /locus_tag="ACHJ48_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO4707110.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFELLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            34966..35928
                     /gene="tauA"
                     /locus_tag="ACHJ48_001525"
     CDS             34966..35928
                     /gene="tauA"
                     /locus_tag="ACHJ48_001525"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4707111.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFSKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            35941..36708
                     /gene="tauB"
                     /locus_tag="ACHJ48_001526"
     CDS             35941..36708
                     /gene="tauB"
                     /locus_tag="ACHJ48_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO4707112.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            36705..37532
                     /gene="tauC"
                     /locus_tag="ACHJ48_001527"
     CDS             36705..37532
                     /gene="tauC"
                     /locus_tag="ACHJ48_001527"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO4707113.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            37529..38380
                     /gene="tauD"
                     /locus_tag="ACHJ48_001528"
     CDS             37529..38380
                     /gene="tauD"
                     /locus_tag="ACHJ48_001528"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO4707114.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(38487..39461)
                     /gene="hemB"
                     /locus_tag="ACHJ48_001529"
     CDS             complement(38487..39461)
                     /gene="hemB"
                     /locus_tag="ACHJ48_001529"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO4707115.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         9114 a        10463 c        10881 g         9155 t
ORIGIN      
        1 atcatgccta caggcctgaa ccgtaggtcg gataaggcgc tcgcgccgca ttcgacacca
       61 tgcgccgatg cctgatgcga cgcaggcgcg tcttatcatg cctacaggcc tgaaccgtag
      121 gtcggataag gcgctcgcgc cgcatccgac accatgcgca gatgcctgat gcgacgctga
      181 tgcgtcttat catgcctaca ggcctgaacc gtaggtcgga taaggcgctc gcaccgcatc
      241 cgacaccgtg ctcagtgcct gatgcgacgc tgatgcgtct tatcatgcct acaggcctga
      301 accgtaggtc ggataaggcg ctcgcgccgc atccgacacc gtgcgcagat gcctgatacg
      361 acgcaggcgc gtcttatcat gcctacaggc ctgaaccgta ggtcggataa ggcgctcgcg
      421 ccgcatccga caccgtgcgt agttgcctga tgcgacgctg acgcgtctta tcatgcctac
      481 ccactgtttt tacaccgata attttccccc cacctttttg cactcattca tataaaaaat
      541 atatttcccc acgaaaacga ttgcttttta tcttcagatg aatagaatgc ggcggatttt
      601 ttgggtttca aacagcaaaa agggggaatt tcgtgtcgca agataacaac tttagccagg
      661 ggccggtccc gcagtcggcg cggaaagggg tattggcatt gacgttcgtc atgctgggat
      721 taaccttctt ttccgccagt atgtggaccg gcggcactct cggaaccggt cttagctatc
      781 atgatttctt cctcgcagtt ctcatcggta atcttctcct cggtatttac acttcatttc
      841 tcggttacat tggcgcaaaa accggcctga ccactcatct tcttgctcgc ttctcgtttg
      901 gtgttaaagg ctcatggctg ccttcactgc tgctgggcgg cacccaggtt ggctggtttg
      961 gcgtcggtgt ggcgatgttt gctattccgg tgggcaaggc aaccgggctg gatattaatt
     1021 tgctgattgc cgtttccggt ttactgatga ccgtcaccgt cttctttggc atttcggcgc
     1081 tgacggttct ttcgttgatt gcggttccgg ctatcgcctg tcttggcggt tattccgtgt
     1141 ggctggccgt taacggcatg ggcggcctgg acgccttaaa agcggtcgtt cccgcacaac
     1201 cgttagattt caatgtcgcg ctggcgctgg ttgtggggtc atttatcagt gcgggcacgc
     1261 tcaccgctga ctttgtccgc tttggtcgca atgccaaact ggcggtgctg gtggcgatgg
     1321 tggccttttt cctcggcaac tcgttgatgt ttattttcgg tgcagcgggc gctgcggcac
     1381 tggggatggc ggatatctct gatgtgatga ttgctcaggg cctgctgttg cctgcgattg
     1441 tggtgctggg gctaaatatc tggaccacca acgataacgc gctctatgcg tcgggtttag
     1501 gtttcgccaa cattaccggg atgtcgagca aaaccctttc ggtaatcaac ggtattatcg
     1561 gtacggtctg tgcattatgg ctgtataaca attttgtcgg ctggttgacc ttcctttcgg
     1621 cagctattcc tccagtgggt ggcgtgatca ttgccgacta tctgatgaac cgtcgccgct
     1681 atgagcactt tgcgaccacg cgtatgatga gtgtcaattg ggtggcgatt ctggcggtcg
     1741 ccctggggat tgctgcaggc cactggttac cgggaattgt tccggtcaac gcggtattag
     1801 gtggcgcgct gagctatctg atccttaacc cgattttgaa tcgtaaaacg acagcagcaa
     1861 tgacgcatgt ggaggctaac agtgtcgaat aacgctttac aaacaattat taacgcccgg
     1921 ttgccaggca aagaggggct gtggcagatt catctgcagg acggaaaaat cagcgccatt
     1981 gatgcgcaat ccggcgtgat gcccataact gaaaacagcc tggatgccga acaaggttta
     2041 gttataccgc cgtttgtgga gccacatatt cacctggaca ccacgcaaac cgccggacaa
     2101 ccgaactgga atcagtccgg cacgctgttt gaaggcattg aacgctgggc cgagcgcaaa
     2161 gcgttattaa cccatgacga tgtgaaacaa cgcgcatggc aaacgctgaa atggcagatt
     2221 gccaacggca ttcagcatgt gcgtacccat gtcgatgttt cggatgcaac gctaactgcg
     2281 ctgaaagcaa tgctggaagt gaagctggaa gtcgcgccgt ggattgatct gcaaatcgtc
     2341 gccttccctc aggaagggat tttgtcgtat cccaacggtg aagcgttgct ggaagaggcg
     2401 ttacgcttag gggcagatgt agtgggggcg attccgcatt ttgaatttac ccgtgaatac
     2461 ggcgtggagt cgctgcataa aaccttcgcc ctggcgcaaa aatacgaccg tctcatcgac
     2521 gttcactgtg atgagatcga tgacgagcag tcgcgctttg tcgaaaccgt tgctgccctg
     2581 gcgcaccgtg aaggcatggg cgcgcgagtc accgccagcc acaccacggc aatgcactct
     2641 tataacgggg cgtatacctc acgtctgttc cgcttgctga aaatgtccgg tattaacttt
     2701 gtcgccaacc cgctggtcaa tattcatctg caaggacgat tcgatacgta tccaaaacgt
     2761 cgcggcatca cgcgcgttaa agagatgctg gagtccggca ttaacgtctg ctttggtcac
     2821 gatgatgtct tcgatccgtg gtatccgctg ggaacggcga atatgctgca agtgctgcat
     2881 atggggctgc atgtttgcca gctgatgggc tatgggcaga ttaacgatgg cctgaattta
     2941 atcacccacc acagcgccag gacgttgaat ttgcaggatt acagcattgc cgccggaaac
     3001 agcgccaacc tgattatcct gccggctgaa aatggatttg atgcgctgcg ccgtcaggtt
     3061 ccggtacgtt attcggtacg tggcggcaag gtgattgcca gcacacaacc ggcacaaacc
     3121 accgtatatc tggagcagcc ggaagccatc gattacaaac ggtgatcggc tgggctgtgt
     3181 gttgacaaag cgagcgtagt ttatgccgga tgcggcttga acgccttatc aggcctacgt
     3241 agagcactga actcgtaggc ctgataagcg taacgcatca ggcaattgtg tcagtggtta
     3301 ccgcgattca ttctcgccaa catccgcaca ttcctccaac gccatgtgca aaaatgcctt
     3361 cgcggcggct gtctgccagc tatttttccg ccgcaacaaa accgccgttc tctccagtag
     3421 tggcggggca agaggaatcg ctttaagccc gtcatgttgt gtggcaatcg ctgctggtaa
     3481 caatgtggaa agggaagtgc ggcgaatcag ctccagaacc gcgctaattg agttcgcctc
     3541 aatgaccacc tgtggatgca gccccgcttt ctcgcagtag tggtcaatct gctctctggt
     3601 ggcaaattcc gcgctgagca ggaccagttt ttcatcatgc aagcgactca acgccacctg
     3661 ttcacaggca gccagcggat gatgttgcgc cacgactaac gctaaacttt ctgtcagtaa
     3721 aggaattgcc tccagctccg gcaaatgcac aggcgcgaag gcaatcccaa cgtccagctc
     3781 gtcgcgacaa agcagatctt cgattttctc ctgcgacatt tcctgtagct ggagcgtgat
     3841 gccggggtag cgcgcataga aatccgccat taaggggccg ataaagtagc tcgtaaaggt
     3901 gggggtgacg gcgatacgca gcgatcctct cgtcagatcg gcaacatcat gaatcgcccg
     3961 tttacccgct cccagttcct gtaacgcccg gctggcgtac tgtcgccaga cttctcctgc
     4021 atcagtgaga tgaatcgttc gcccgctacg gtcaaacagc ggcacgccta aactctcctc
     4081 taactggcga atctgctggg aaagcgcagg ctgagagacg tgcaacgcac tggcggcacg
     4141 ggtgaagctg ccatgttcag ccacggcaag aaaataattg atatgtcgag agagcattcg
     4201 ccaacctata agtaaattca atagaactca tcataaatga gacttttacc ttatgacaat
     4261 cggcgagtag tctgcctctc attccagaga cagatagagg ttaacggtga aagagattat
     4321 tgatggattc cttaaattcc agcgcgaggc atttccgaag cgggaagcct tatttaaaca
     4381 gttggcgaca cagcaaagcc cgcgcacact ttttatctcc tgctccgaca gccgtctggt
     4441 ccctgagctg gtgacgcaac gtgagcctgg cgatcttttc gttattcgca atgcgggcaa
     4501 tatcgtccct tcctacgggc cggaacccgg tggcgtttct gcttcggtgg agtatgccgt
     4561 cgctgcgctt cgggtatctg acattgtgat ttgcggtcat tccaactgtg gcgcgatgac
     4621 cgccattgcc agctgtcagt gcatggacca tatgcctgcc gtctcccact ggctgcgtta
     4681 tgccgattca gcccgcgtcg ttaatgaggc gcgcccgcat tccgatttac cgtcaaaagc
     4741 tgcggcgatg gtacgtgaaa acgtcattgc tcagttggct aatttgcaaa ctcatccatc
     4801 ggtgcgcctg gcgctcgaag aggggcggat cgccctgcac ggctgggtct acgacattga
     4861 aagcggcagc atcgcagcgt ttgacggcgc aacccgccag tttgtgccac tggcagctaa
     4921 tcctcgcgtt tgtgccatac cgctacgcca accgaccgca gcgtaacctt atttttaaac
     4981 catcaggaga tgcaccatga ttcagtcgca aattaaccgc aatattcgtc ttgatcttgc
     5041 cgatgccatt ttgctcagca aagctaaaaa agatctctca tttgccgaga ttgccgacgg
     5101 caccgggctg gcagaagcct ttgtgaccgc ggctttgctg ggtcagcagg cgcttcctgc
     5161 cgacgccgcc cgccaggtcg gggcgaagct ggatctcgac gaagacgcca ttctactgtt
     5221 acagatgatc ccactgcgtg gctgcattga tgaccgtatt ccaaccgacc caacgatgta
     5281 tcgtttctat gaaatgttgc aggtgtacgg cacaaccctg aaagcgttgg ttcatgagaa
     5341 atttggcgat ggcattatca gcgcgattaa cttcaaactc gacgttaaga aagtggcgga
     5401 cccggaaggt ggcgaacgtg cggtcatcac cttagatggt aaatatctgc cgaccaaacc
     5461 gttctgacag ccatgcgtaa ccatcaaaag acgttcacga tgctgctggt tctggtgctg
     5521 attggtctta atatgcgacc actgctcacc tccgtcgggc cactgctacc gcaattgcgc
     5581 caggcaagcg gaatgagctt tagcgtggct gccctgttga ccgctctgcc ggtggttacc
     5641 atgggcgggc tggcgctggc cggaagctgg cttcatcagc atgtcagcga acgtcgcagc
     5701 gtcgccatca gtttgttact gattgccgtc ggtgcattga tgcgtgagct ttaccctcaa
     5761 agtgcgctgc tgcttagcag cgcactgctc ggtggggtgg ggatcggcat cattcaggcg
     5821 gtgatgcctt cggtgattaa acggcggttt cagcagcgca cgccactggt gatggggctg
     5881 tggtccgcgg ctctgatggg cggcggtggg cttggtgccg ccctgacacc ctggttagtt
     5941 caacatagcg aaacctggta tcaaacactc gcctggtggg cgctgcctgc cgttgttgcg
     6001 ctctttgcct ggtggtggca aagcacccgc gaggtcgcct cttcccacaa gacaacaacc
     6061 attccggttc gcgtggtttt cactcctcgc gcgtggacgc tgggtgttta cttcggtctg
     6121 attaacggtg gttacgccag cctgattgcc tggttacccg ccttctatat tgagattggt
     6181 gccagcgcgc agtacagcgg ttccttgctg gcattgatga cgcttgggca agccgcagga
     6241 gctttgctga tgcctgctat ggctcgccat caggatcggc gcaaactgtt aatgctggcg
     6301 ctggcgttac aactggtggg tttctgcggc tttatctggc tgccgctgca attgccggta
     6361 ttgtgggcga tggtgtgtgg tttaggtctg ggcggcgcat ttccgctctg tttgctgctg
     6421 gcgctcgatc actctgtgca accggctatt gctggcaagc tggtggcgtt tatgcaggga
     6481 atcggtttta tcatcgccgg gcttgccccg tggttttctg gcgtgctgcg tagtatcagc
     6541 ggcaattact tgatggactg ggcgtttcat gcgctgtgcg tcgtagggct gatgattata
     6601 accctgcgtt ttgcaccagc acgttttccg cagctgtggg tcaaagaggc atgatgcgac
     6661 gcttgtttct gcgctttgtt cacgccggat gcggcctaca tagatcactg aatttgtagg
     6721 cctgataagc gaagcgtatc aggcaatttt tataatttaa actgacgatt caactttgta
     6781 atctctgaaa taatattgct tatcccggtc gtttatttcg cgaataaccc gacaaggaac
     6841 gccagccgcc acgacgtttg gtggaatgtc ttttgtgacg atactacccg cgccaataac
     6901 agaattatcc ccaatggtga cgcctggatt aataaccaca tgacttccga tccagacgtt
     6961 attgccaatc gttatcggaa aagagtacat ctcaccgttt tttctcaatt catggtgtac
     7021 agggtgtccc gtaacggaaa gagtaacgtt gggtgcaatc aatacgttat caccgattgt
     7081 taccgtgtag tcatcgacaa tggttaaatt gaaatttgca taaaaattgc ggcctatatg
     7141 gatgttggaa ccgtaagaga aatagacggg gggttctacc caggcgtttt cccctaccgt
     7201 ggcaaacatt tctttaatca ggctttctct tttatcaact tctgatggat gcgagtgatt
     7261 aaactcatac attaacgttt ttctacgaag tcttttttca ggtaagcctt cgcacatatc
     7321 ggtaaatagc ttgcctgctt ttattctttc tgtcatcgac atgttcattg cgatcattcc
     7381 gttatgatat gttggtcgga taaggcgctc gcgccgcatc cgacattgat tgcttaagcg
     7441 acttcattca cctgacgacg cagtagagaa agcgggccgg ggccgctaag cgtgaacacg
     7501 gaaattaagg tgaagcccag cgccaccaga cccagcacca gataagcgcc ctggaaaccg
     7561 atgctttcat acatattacc cgctagtaca gacataaaaa tcatcgccag ttgcttaaag
     7621 aagcagaaac agaccagata aatcgtcgct gaaaaacgca cttcaaactg gctggtaata
     7681 tatttaaagc agcccaccag caggaacggt acttcaaaca tatgcagcgt tttcagaata
     7741 accacttcca gcgctgaggt ggcgaacgat gagccaataa tacgtacaga cataatagtg
     7801 ccagccagca gcagggcatt tttcccaccg atgcgattaa tgatcagtgg cgcaaagaac
     7861 ataattgagg cgttaagtaa ttcgcccatt gtcgttacgt agccaaatac ccgcgtaccc
     7921 tgttcaccgg tggcaaagaa agaagtaaag aaattagcaa actgttggtc aaaaacatcg
     7981 taggtgcagg aaacgccaat aacatacagt gacaaaaacc acagttttgg ctgtctgaac
     8041 agttccagcg ccagtttaag gctaaatgcc gaatggttgg cacctaccgc attggcaacc
     8101 gtggcggaag agggcgcatc cgttttggcg aaaaagagta aaatggcgag gatgagtgca
     8161 cagccagaac ccagccagaa aacgaactga ttattgatgg tgaacatgat gccgacaatc
     8221 gaggcacaca gcgcccagcc aacacagcca aacatccgcg cgcgaccaaa ttcgaaatta
     8281 ctgcgacggc tgactttctc gataaatgcc tctactgcgg gcgcaccggc gttaaaacaa
     8341 aagccaagat aaataccacc aacaatcgat cctactaaaa tgttgtattg taacagtggc
     8401 ccgaagataa aaataaagaa cggcgcaaac atcactaaca tgccggtaat aatccacagc
     8461 aggtatttgc gcagcccgag tttgtcagaa agcagaccaa acagcggttg gaataatagc
     8521 gagaacagag aaatagcagc aaaaataata cccgtatcac ttttgctgat atggttgatg
     8581 tcatgtagcc aaatcgggaa aaacgggaag taggctccca tgataaaaaa gtaaaagaaa
     8641 aagaataaac cgaacatcca aaagtttgtg ttttttaaat agtacataat ggatttcctt
     8701 acgcgaaata cgggcagaca tggcctgccc ggttattatt atttttgaca ccagaccaac
     8761 tggtaatggt agcgaccggc gctcagctgg aattccgccg atactgacgg gctccaggag
     8821 tcgtcgccac caatccccat atggaaaccg tcgatattca gccatgttcc ttcttccgcg
     8881 tgcagcagat ggcgatggct ggtttccatc agttgttgtt ggctgtagcg gctgatgttg
     8941 aactggaagt cgccgcgcca ctggtgtggg ccataattca attcgcgcgt cccgcagcgc
     9001 agaccgtttt cgctcgggaa gacgtacggg gtatacatgt ctgacaatgg cagatcccag
     9061 cggtcaaaac aggcggcagt aaggcggtcg ggatagtttt cttgcggccc taatccgagc
     9121 cagtttaccc gctctgctac ctgcgccagc tggcaggtca ggccaatccg tgccggatgc
     9181 ggtgtatcgc tcgctacttc aacatcaacg gtaatcgcca tttgaccact accatcaatc
     9241 cggtaggttt tccggctaat aaataaggtt tttccctgat gctgccatgc gtggacagtg
     9301 gtaatcagca ccgcgtcggc aagtgtatct gccgtgcact gcaacaacgc cgcttctgcc
     9361 tggtaatggc ccgcagcctt ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct
     9421 tcacttacgc caatgtcgtt atccagcggt gcgcgggtga actgatcgcg cagcggcgtc
     9481 agcagttgtt ttttatcgcc aatccacatc tgtgaaagaa agcctgactg gcggttaaat
     9541 tgccaacgct tattatccag ctcgatgcaa aaatccgttt cgctggtggt cagttgcggg
     9601 atggcgtggg gcgcggaggg gagtgtcacg ctgaggtttt ccgccagacg ccactgctgc
     9661 caggcgctga tgtgtccggc ttctgaccat gcggtcgcgt tcggttgcac tacgtgtacc
     9721 gttagccaga gttgcccggt gctctccagt cgcggtagtt caggcaattc aattacctgt
     9781 ttaccttgcg gagcgacatc cagaggcact tcaccgcttg ccagcggctt accatccagc
     9841 gccaccgtcc agtgcaggag ctcgttatcg ctatgacgga acaggtattc gctggtcact
     9901 tcgatggttc gcccggataa acggaactgg aaaaactgct gctggtgttt tgcttccgtc
     9961 agcgccggat gcggcgtgcg gtcggcaaag accagaccgt tcatgcagaa ctggcgatca
    10021 ttcggcgtat cgccaaaatc accgccgtaa gccgaccacg gattgccatt ttcatcatat
    10081 ttaatcagcg actgatccac caagtcccag acgaagccgc cctgtaaacg ggggtactga
    10141 cgaaacgcct gccagtattt agcgaagccg ccaagactgt tacccatcgc gtgggcgtat
    10201 tcgcaaagga tcagtgggcg catttctcca ggcagcgaaa gccatttttt gatggaccat
    10261 ttcggcacag ccgggaaggg ctggtcttca tccacgcgcg cgtacatcgg gcaaataata
    10321 tcggttgcgg aggtgtcggc tccgccgcct tcatactgca ccgggcggga tggatcgaca
    10381 gatttaatcc agcgatagag tgcgtcgtga ttagcgccgt ggcctgactc attccccagc
    10441 gaccagatga tcacactcgg gtgattacga tcgcgctgca ccattcgtgt tactcgttcg
    10501 ctcatggccg gtaaccagcg cggatcatcg gtcagacgat tcattggcac catgccgtgg
    10561 gtttcaatat tggcttcatc caccacatac aggccgtagc ggtcgcacag ggtgtaccac
    10621 agcggatggt tcggataatg cgaacagcgc acggcgttaa agttgttctg cttcattagc
    10681 aggatatcct gcaccatcgt ctgctcatcc atgacctgac catgcagagg atgatgctcg
    10741 tgacggttaa cgccgcgaat cagcaacggc ttgccgttca gcagcagcag accattttca
    10801 atccgcacct cgcggaaacc gacatcgcag gcttctgctt caatcagcgt gccgtcggcg
    10861 gtgtgcagtt caaccaccgc acgatagata ttcgggattt cggcgctcca cagtttcggg
    10921 ttttcgacgt tcagacctag tgtgacgcga tcggcataac cgccacgctc atcgataatt
    10981 tcaccgccga aaggcgcggt gccgctggcg acctgcgttt caccctgcca caaagaaacc
    11041 gtcacccgca gctcatcgcg cagctcgccg tacatctgaa cttctgcctc cagtaccgcg
    11101 cggctaaaat catcattaaa gagagtggca acatggaaat cgctgatttg tgtggtcggt
    11161 ttgtgcagca acgagacgtc acggaaaatg ccgctcatcc gccacatatc ctgatcttcc
    11221 agataactgc cgtcactcca gcgcagcacc atcaccgcga ggcggttttc tccggcgcgt
    11281 aaaaatgcgc tcaggtcaaa ttcagacagc aaacgactgt cctggccgta accgacccag
    11341 cgcccgttgc accacagatg aaacgccgag ttaacgccat caaaaataat tcgcgtctgg
    11401 ccttcctgta gccagctttc atcaacatta aatgtgagcg agtaacaacc cgtcggattc
    11461 tccgtgggaa caaatggcgg attgaccgta atgggatatg tcacgttggt gtagatgggc
    11521 gcgtcgtaac cgtgcatctg ccagtttgag gggacgacga cagtatcggc gtcaggaaga
    11581 tcgcactcca gccagctttc cggaaccgct tctggtgccg gaaaccagac aaattgccac
    11641 tcaccattca ggctgcgcaa ctgctgggaa gggcgattgg tgcgggcctc ttcgctatta
    11701 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    11761 tcccagtcac gacgttgtaa tacgacggcc agtgaatctg taatcatagt catagctgta
    11821 tcctgtgtga aattgttatc cgctcacaat ttcacacaac atacgagccg gacgcataga
    11881 gtgtaaagcg tggggtgcct aatgagtgag ctgactcaca ttaattgcgt tgcgctcact
    11941 gcccgctttc cagtcgggaa acttgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    12001 ggggagaagc ggtttgcgtc ttgggcgcca gggtggtttt tcttttcacc agtgagacgg
    12061 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
    12121 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacacg
    12181 agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcaacccgg
    12241 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
    12301 tgggaacgat gccctcattt agcatttgca tggtttgttg aaaaccggac atggcactcc
    12361 agtcgccctc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
    12421 ccgccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
    12481 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa
    12541 taatactggt gatgggagtc tggtcagaga catcaagaaa taacgccgga acattagcgc
    12601 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
    12661 tgacgcgttg cgcgagaaga ttgtgcactg ccgctttaca ggcttcgacg ccgcttcgtt
    12721 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
    12781 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
    12841 gtttccccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
    12901 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctgattcacc acgcgggaaa
    12961 cggtctgata agagacaccg gcatactctg cgacatcgta tagcgttact ggtttcacat
    13021 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
    13081 gccattcggc ggggctgacg ccgaagtgag atttaaaatg ctgacgccag gtcacaggtg
    13141 agagaaaacc cacctcagcc gctatccgct caacgggcaa ctgactggat cccaacagtt
    13201 cctggctacg gcgtaagcgt tcagtaatga gccattcggc gatactcgaa ccggtcgctt
    13261 tcataaaatg gcgagttaat gtgcggcgac tcatcattac tcgctgcgcc agcgagtcga
    13321 gatcatgctg ttgcgcaatg ttttgccgca ggtaatccag caggaggttt atgcgttcat
    13381 catgggtatt tttcggcacc ggttgctcaa taaactgcgc ctgaccgccg gtgcgatatg
    13441 gcggtacgac catccgtcgg gcaatatggt tagcatagtc gctgccaaaa tgttgccgaa
    13501 caatatacaa acagcaatcc agcgccgcgg cagtaccggc agaggtaata atgccgtcat
    13561 cgtctacgta caacgcgtta atatccagat gtgtttgtgg aaaacgtgcc tgaaattcac
    13621 gctcgaactc ccagtgcgtg gcggcacgct tatttttcag taaacctgca tagccgagca
    13681 caaacgtgcc caggcaaagc ccgacaatct gtgcgccgtt atcccgtgcg gtcgttaagg
    13741 cttccagcag tttttgtggc ggtttttgtg taatggttcc ccagtaagga attatcacaa
    13801 tatctgcctg gatgactgcg gtatagtcat gggtggcggt tacggaaaac ccatcggcag
    13861 agtccacgtt tcccggcttt tcagcgcaaa taatcacgtg aaagcgtttt ttctccgcca
    13921 tcttttcact aaacacgata aacggtacgg aaaggtgaaa aggactaaaa ccggcaacgg
    13981 cgataatggc aacagttagc gggcgcataa cactctttat ctccgaatta gaatgtcaac
    14041 gtttcaccat cttctggtac gttcacaaat tcgataattt gattatctct ggcgtattcc
    14101 ttgagcgcat cacgcgtcag caaacagtgg tttacggcct ccatatgtgt cgcgataatt
    14161 tgcgcttctg ggagggtaaa atgtgttttg agaatatctt ctgctcccat aattatcgcg
    14221 ccatgcccca gaacatgcgc aaatccggca ttcaacacaa ccacttctgg ttggaaagtt
    14281 agcattgtag cctcgacgtc gtcacgccag agggtatcgc cagccagata gagcgttttc
    14341 tctgtcgggt gctggaaaac aacgccgcaa gctgcgccta aacgcttcgc catcgccggg
    14401 tcggcatagg tgcgatcgga gccgtgctgc cctgtcgttt tatgcagagt aatatcgcca
    14461 aatttagtct cgtcggtaag tacggtaaga tgcgtaaatc cttgtttatt cagtgattca
    14521 gcatcttgct cattttgcac ataaatcagt ttatctttag ggacgacgcg ggaggctgtt
    14581 tcgtcccagt gatcttcatg caaatgggtc acgattaggg cgtcaacgtt gagtaacgtt
    14641 gttaaatcgc acggtaattc taccgttgga ttgcggattt ctgcgcgtgc cgttccggca
    14701 aaacccggat aacttccttt aggcgcgagt aacgggtcga taagaaatgt tttgccagcg
    14761 tattggataa tttgggtggc attacgaatt tgcgtaattt tcatgatttg ctccacatca
    14821 cagtattgat gtcattagcg tagcgtgcag cccgtactgt gcatgtgggc aaatgggcga
    14881 ataacgatga gaatgggcca aattacacat tggcgtaact agcgtaaatg cacgccgctt
    14941 cgccgtccgg ccaccagaat agcctgcgat tcaacccctt cttcgatctg ttttgctacc
    15001 cgttgtagtg ccggaagatg cttttccgct gcctgttcga tggtcattgc gctcgccata
    15061 tacaccagat tcagacagcc aatcacccgt tgttcactgc gcagcggtac ggcgatagag
    15121 gcgatcttct cctcctgatc ccagccgcgg tagttctgtc cgtaaccctc tttgcgcgcg
    15181 cgcgccagaa tggcttccag ctttaacggt tctcgtgcca gttgatagtc atcaccgggg
    15241 cgggaggcta acatctcgat taattccttg cgttcttgtt ccgggcaaaa ggccagccag
    15301 gtcaggcccg aggcggtttt cagaagcggt aaacgccgcc cgaccattgc ccggtgaaag
    15361 gataagcggc tgaaacggtg agtggtttcg cgtaccacca ttgcatcaac atccagcgtg
    15421 gacacatctg tcggccatac cacttcgcgc aacagatcgc ccagcagtgg ggccgccagt
    15481 gcagaaatcc actgttcgtc acgaaatcct tcgcttaatt gccgcacttt gatggtcaga
    15541 cgaaaactat catcggaggg actacgccgg acatatccct cttcctgcag cgtctccagc
    15601 agtcgccgca cagtggtgcg atgcaggccg ctgagttccg ccagcagccc gacgctggca
    15661 ccgccgtcaa gtttatttaa catatttaat aacattagac cgcgggttaa gccgcgcacg
    15721 gttttgtatt ccgtctgctc attgttctgc atattaattg acatttctat agttaaaaca
    15781 acgttgtgca cctggtgcac attcgggcat gttttgattg tagccgaaaa caccctttct
    15841 atactgagcg cacaataaaa aatcatttac atatttttaa caaaataagt tgcgctgtac
    15901 tgtgcgcgca acggcatttt gtccgagtcg tgaggtactg aaatggcaat acaacaccct
    15961 gacatccagc ctgctgttaa ccatagcgtt caggtggcga tcgctggtgc cggtccggtc
    16021 gggctgatga tggcgaacta tctcggccag atgggcattg acgtgctggt ggtggagaaa
    16081 ctcgataagt tgatcgacta cccgcgtgcg attggtattg atgacgaggc gctgcgcacc
    16141 atgcagtcgg tcggcctggt cgataatgtt ctgccgcata ctacgccgtg gcacgcgatg
    16201 cgttttctca ccccgaaggg ccgctgtttt gctgatattc agccaatgac cgatgaattt
    16261 ggctggccgc gccgtaacgc ctttattcag ccgcaggtcg atgcggtgat gctggaaggg
    16321 gtgtcgcgtt ttccgaatgt gcgctgcttg ttttcccgcg agctggaggc cttcagtcag
    16381 caagatgacg aagtgacctt gcacctgaaa acggcagaag ggctgcggga aatagtcaaa
    16441 gcccagtggc tggtggcctg tgatggtggg gcaagttttg tccgtcgcac cctgaatgtg
    16501 ccgtttgaag gtaaaactgc gccaaatcag tggattgtgg tagatatcgc caacgatccg
    16561 ttaagtacgc cgcatatcta tttgtgttgc gatccggtgc gcccgtatgt ttctgccgcg
    16621 ctacctcatg cggtacgtcg ctttgaattt atggtgatgc cgggagaaac cgaagaacag
    16681 ctgcgtgagc cgcaaaatat gcgcaagctg ttaagcaaag tgctgcctaa tccggacaat
    16741 gttgaattga ttcgccagcg tgtctacacc cacaacgcgc gactggcgca acgtttccgt
    16801 attgatcgcg tactgctggc gggcgatgcc gcgcacatca tgccggtatg gcaggggcag
    16861 ggctataaca gtggtatgcg cgacgccttt aacctcgcat ggaaactggc gttggttatc
    16921 caggggaaag cccgcgatgc gctgctcgat acctatcaac aagaacgtcg cgatcacgcc
    16981 aaagcgatga ttgacctgtc cgtgacggcg ggcaacgtgc tggctccgcc gaaacgctgg
    17041 cagggtacgt tacgtgacgg cgtttcctgg ctgctgaatt atctgccgcc agtaaaacgc
    17101 tacttcctcg aaatgcgctt caagccgatg ccgcaatatt acggcggtgc gctggtgcgt
    17161 gagggcgaag cgaagcactc tccggtcggc aagatgttta ttcagccgaa agtcacgctg
    17221 gaaaacggcg acgtgacgct gctcgataac gcgatcggcg cgaacttcgc ggtaattggc
    17281 tggggatgca atccactgtg ggggatgagc gacgagcaaa tccagcagtg gcgcgcgttg
    17341 agcacacgct tcattcaggt ggtgccggaa gtgcaaattc ataccgcaca ggataaccac
    17401 gacggcgtac tacgcgtggg ggatacgcaa ggtcgcctgc gtagctggtt cgcgcaacac
    17461 aatgcttcgc tggtggtgat gcgcccggat cgctttgttg ccgccaccgc cattccgcaa
    17521 acactgggca agaccctgaa taaactggcg tcggtgatga cgctgacccg ccctgatgcc
    17581 gacgtttctg tcgaaaaggt agcctgatat gcacgcttat cttcattgtc tttcccactc
    17641 gccgctggtg gggtatgtcg acccggcaca agaggtactc gatgaggtca atggcgtgat
    17701 tgccagcgcc cgcgagcgta ttgcggcttt ctcccctgaa ctggtggtgc tgtttgcgcc
    17761 agatcactac aacggctttt tctatgacgt gatgccaccg ttctgtttag gcgttggagc
    17821 gacggcaatt ggtgatttcg gcagtgcggc aggagagctg cccgtgcctg tggagctggc
    17881 ggaggcctgt gcgcatgccg tcatgaagag cgggatcgat cttgccgttt cttactgtat
    17941 gcaggtggac cacgggttcg cccagccgct ggagttcctg ctcggtgggc tggataaggt
    18001 gccagttctg cctgtgttca tcaacggtgt cgccacgccg ctgcccggtt tccagcgtac
    18061 ccgcatgttg ggtgaagcca ttggacgttt caccagcact ctcaataaac gcgtgctgtt
    18121 cctaggttcc ggtgggcttt cccatcagcc gccggtgccc gaactggcga aagccgatgc
    18181 ccatatgcgc gaccgcctgt tggggagcgg gaaagattta cccgccagtg agcgcgaatt
    18241 gcgtcagcaa cgggtgatta gcgccgctga gaagtttgtt gaggatcaga gaactctgca
    18301 tccgctcaac ccgatttggg ataaccagtt catgactttg ctggagcagg gacgcataca
    18361 ggaactggat gccgtcagta acgaagagct ttccgccatt gccggaaagt cgacacatga
    18421 aatcaaaacc tgggtcgccg cttttgccgc tatttctgca tttggcaact ggcgtagcga
    18481 agggcgttat taccgcccaa tcccggagtg gattgccgga tttggctcgt taagcgccag
    18541 aacagagaac tgaatatgca ggagaagatg atgagttatc agccacaaac cgaagccgcc
    18601 accagccgtt ttctgaatgt agaagaagcg ggtaaaacgc tgcgcatcca ttttaatgac
    18661 tgcggacaag gcgacgaaac cgttgtcctg ctgcatggtt ccggcccggg tgctactggc
    18721 tgggcgaact tcagccgcaa tatcgatccg ctggtagagg cgggctatcg ggtgatcctg
    18781 ctggattgtc cgggttgggg caagagcgat tcgatcgtta atagtggttc gcgatcggat
    18841 cttaatgcac gaatcctgaa aagcgtggtg gatcaactgg atatcgccaa aatccacctg
    18901 ctgggcaact cgatgggtgg ccatagttct gtggcgttca cccttaactg gccggagcgc
    18961 gtcggcaaac tggtgctgat gggcggcggt acgggcggca tgagtttgtt tacgccgatg
    19021 ccaaccgaag gtattaagcg actgaatcag ctttatcgtc agccgactat cgaaaacctg
    19081 aagctgatga tggatatctt cgtttttgat accagcgatt tgaccgacgc cctgtttgaa
    19141 gcgcgcctga ataatatgct gtcgcgccgc gatcacctgg aaaacttcgt taagagcctg
    19201 gaagctaatc cgaaacagtt cccggatttt ggcccacgtc tggcggaaat caaagcgcaa
    19261 accctgattg tctgggggcg caacgaccgc tttgtgccga tggatgcggg tctgcgtctg
    19321 ctgtccggca ttgccggttc tgaactgcat atcttccgcg actgtgggca ttgggcgcag
    19381 tgggaacatg ccgacgcttt caatcaactg gtgctgaatt tcctcgcacg tgcttaagga
    19441 acggtcatga cgaagcatac tcttgagcaa ctggcggcgg atttacgccg cgccgcagag
    19501 cagggcgaag cgattgcgcc gctgcgcgat ctgattggta tcgataacgc tgaagcggcc
    19561 tacgccattc agcacataaa tgtgcaatat gacgttgcgc aggggcgtcg cgtggtgggg
    19621 cgtaaagtgg gcctgacaca tccgaaagtg cagcaacaac tgggcgttga tcaaccggat
    19681 ttcgggacat tatttgccga catgtgttat ggcgataacg aaatcattcc tttttcccgt
    19741 gttctgcaac cccgcattga agcggagatc gcactggtgt tgaaccgcga tttacccgca
    19801 actgatatca ccttcgacga attgtataac gccattgaat gggtacttcc ggcgctggag
    19861 gtggtgggta gccgcattcg cgactggtcg attcagtttg tcgataccgt ggcagataac
    19921 gcttcctgcg gggtgtatgt catcggcggt ccggcgcaac gtccggcggg gttagacctg
    19981 aaaaactgcg ccatgaagat gacgcgtaat aacgaagagg tttccagcgg gcgcggcagc
    20041 gaatgcctgg gacatccgct taatgccgcc gtctggctgg cacgcaaaat ggccagtctg
    20101 ggtgaaccgc tgcgcgccgg agatatcatt cttaccgggg cattaggtcc gatggtggca
    20161 gtgaatgcgg gcgatcgttt tgaagcccat attgaaggca taggttcagt tgctgcggca
    20221 ttttcaagcg cagccccaaa aggaagtctg tcatgagtaa gcgtaaagtc gccattatcg
    20281 gttctggcaa cattggcacc gatctgatga ttaaaatttt gcgtcacgat cagcatctgg
    20341 agatggcggt gatggtcggc attgatcctc agtccgacgg tctggcgcgt gccagacgta
    20401 tgggcgtcgc caccacccat gaaggggtga tcgggctgat gaacatgcct gaatttgctg
    20461 atatcgacat tgtatttgat gcgaccagcg ccggtgctca tgtgaaaaac gatgcggctt
    20521 tgcgcgaagc gaaaccggat attcgcttaa ttgacctgac gcctgctgcc atcggccctt
    20581 actgcgtgcc ggtggttaac ctcgaggaga acgtcgatca actgaacgtc aacatggtca
    20641 cctgcggcgg ccaggccacc attccaatgg tggcggcagt ttcacgcgtg gtgcgtgttc
    20701 attacgccga aattatcgct tctatcgcca gtaaatctgc cggacctggc acgcgtgcca
    20761 atatcgatga atttacggaa accacttcgc gagccattga agtggtgggc ggcgcggcaa
    20821 aagggaaggc gattatcgtg cttaacccag cagagccacc gttgatgatg cgtgacacgg
    20881 tgtatgtatt gagcgacgaa gcttcacaag atgatatcga agcctcaatc aatgaaatgg
    20941 ctgaggcggt gcaggcttac gtaccgggat atcgcctgaa acagcgcgtg cagtttgaag
    21001 ttatcccgca ggataaaccg gttaatttgc cgggcgtagg gcagttctcc gggctgaaaa
    21061 cggcggtctg gctggaagtc gaaggcgcag cgcattatct gcctgcctat gcgggcaacc
    21121 tcgacattat gacttccagt gcgctggcga cagcagaaaa aatggcccag tcactggcgc
    21181 gcaaggcagg agaagcggca tgaacggtaa aaaactttat atctcggacg tcacgttgcg
    21241 tgacggtatg cacgccattc gtcatcagta ttcgctggaa aacgttcgcc agattgccaa
    21301 agcactggac gatgcccgcg tggattcgat tgaagtggcc cacggcgacg gtttgcaggg
    21361 gtccagcttt aactatggtt tcggcgcaca tagcgacctt gaatggattg aagcagcggc
    21421 ggatgtggtg aagcacgcca aaatcgcgac gttgttgctg ccaggaatcg gcactattca
    21481 cgatctgaaa aatgcctggc aggctggcgc gcgggtggtt cgtgtggata cgcactgtac
    21541 cgaagctgat gtttccgccc agcatattca gtatgcccgc gagctcggaa tggacaccgt
    21601 tggttttctg atgatgagcc atatgaccac gccggagaat ctcgcaaagc aggcaaagct
    21661 gatggaaggc tacggtgcga cctgtattta tgtggtggat tctggcggtg cgatgaacat
    21721 gagcgatatc cgtgaccgtt tccgcgccct gaaagcagtg ctgaaaccag aaacgcaaac
    21781 cggcatgcac gctcaccata acctgagtct tggcgtggcg aactctatcg cggcggtgga
    21841 agagggctgc gaccgaatcg acgccagcct cgcgggaatg ggagcgggcg caggtaacgc
    21901 gccgctggaa gtatttattg ccgccgtgga taaactgggt tggcagcatg gggccgatct
    21961 ctatgcgtta atggatgctg ctgacgacct ggtgcgtccg ttgcaggatc gaccggtacg
    22021 agtcgatcgc gaaacgctgg cgctgggata cgctggtgtt tactccagct tcctgcgtca
    22081 ctgtgaaacg gcggcggcgc gttatggctt aagcgcggtg gatattctcg ttgagctggg
    22141 caaacgccgg atggttggcg gccaggagga tatgatcgtc gacgtggcgc tggatctgcg
    22201 caacaacaaa taatattgac tgccgagaac gtgcattttg tatgcccatc ccagcacgac
    22261 tgccggatgc ggcgtgaacg ccttatccgg tctacatttc gcccgtaggc agtcattaaa
    22321 tagttctgat tacgcggctg gcaatgtatc agtcgcgatt cacctgcaca cgcaatgagg
    22381 ttcatcttat gtcgactcgt accccttcat catcttcatc ccgcctgatg ctgaccatcg
    22441 ggctttgttt tttggtcgct ctgatggaag ggctggatct tcaggcggct ggcattgcgg
    22501 cgggtggcat cgcccaggct ttcgcactcg ataaaatgca aatgggctgg atattcagcg
    22561 ccggaatact cggtttacta cccggcgcgt tggttggcgg aatgctggcg gaccgttatg
    22621 gtcgcaagcg cattttgatt ggctcagttg cgctgtttgg tttgttctcg ctggcaacgg
    22681 cgattgcctg ggatttcccc tcactggtct ttgcgcggct gatgaccggt gtcgggctgg
    22741 gggcggcgtt gccgaatctt atcgccctga cgtctgaagc cgcgggtcca cgttttcgtg
    22801 ggacggcagt gagcctgatg tattgcggtg ttcccattgg cgcggcgctg gcggcgacac
    22861 tgggtttcgc gggggcaaac ttagcatggc aaacggtgtt ttgggtaggt ggtgtggtgc
    22921 cgttgattct ggtgccgcta ttaatgcgct ggctgccgga gtcggcggtt ttcgctggcg
    22981 aaaaacaggc tgcgccacca ctgcgtgcgt tatttgcgcc agaaacggca accgcgacgc
    23041 tgctgctgtg gctgtgttat ttcttcactc tgctggtggt ttacatgttg atcaactggc
    23101 taccgctgct tttggtggag caaggattcc agccatcgca ggcggcaggg gtgatgtttg
    23161 cactgcaaat gggtgcggcg agcgggacgt taatgttggg cgcattgatg gataagctgc
    23221 gtccagtaac catgtcgcta ctgatttata gcggcatgtt agcttcgctg ctggcgcttg
    23281 gaacggtgtc gtcatttaac ggtatgttgc tggcgggatt tgtcgcgggg ttgtttgcga
    23341 caggtgggca aagcgttttg tatgccctgg caccgttgtt ttacagttcg cagatccgcg
    23401 caacaggtgt gggaacagcc gtggcggtag ggcgtctggg ggctatgagc ggtccgttac
    23461 tggccgggaa aatgctggca ttaggcactg gcacggtcgg cgtaatggcc gcttctgcac
    23521 cgggtattct tgttgctggg ttggcggtgt ttattttgat gagtcggaga ttaagaatgc
    23581 agccgtgtgc cgatgcctga cgcgccttat taagcagggg cgaaagggac ttgcccccaa
    23641 tacacccacg gcgtttacag gtatactcgc taaaaattat tcagcgggtt tggaaacaaa
    23701 gatggcaaaa cttaccttac aagagcagtt gctcaaagca ggattagtca ccagcaaaaa
    23761 agcggcgaag gtcgagagaa cggcgaaaaa atcgcgcgtt caggcgcgtg aagctcgggc
    23821 ggcggtagaa gaaaataaaa aggcacagct tgagcgtgat aaacagctta gcgaacagca
    23881 aaaacaagcg gcgttggcga aagaatataa agctcaggtg aagcagctta ttgaaatgaa
    23941 ccggatcacc attgccaatg gcgatattgg ttttaacttc actgatggca atctgattaa
    24001 gaagattttt gtcgataagc tcacacaggc gcagttgatt aatggtcgtc tggcgattgc
    24061 ccgcttgttg gtcgataaca atagcgaagg tgaatacgcc attattcccg ccagcgttgc
    24121 cgataaaatt gctcagcgtg atgccagcag tattgtgttg cacagcgcgc tcagtgccga
    24181 agagcaggat gaagacgacc cgtatgccga cttcaaagtg cctgatgatt tgatgtggta
    24241 aacatagata agtcgtgcgc agatgcctga tgcgacgcta tgcgcgtctt atcatgccta
    24301 cggcctgcgg gcgaaatgta ggcggataag gcgttcacgc cgcatccggc agtcgtgcac
    24361 tattatcaac gcatattcag tttattggcg tggtaggcaa tatgctcgcc aataaaactg
    24421 gagacaaaat aatagctgtg gtcgtaaccc tcgtgataac ggattaacgt tttgatattc
    24481 atctcctggc agatcttttc aagattttga gtccgcagct gttccgcgta aaaatcatca
    24541 ctcaacccct gatcaaccat gatttccgca acgcgttgac cttgtgaaat aagactcacc
    24601 ggatggtaat ccaaccaggc ctctttattt tcaccaagat atgcggcaaa ggcttgctgt
    24661 ccccacggta cttgcgatgg ggagacaatg ggcgaaaacg ccgagacgct gacatattca
    24721 tctgggttac gtaacgccag caccagcgcg cccagtccgc ccatagaatg accagagata
    24781 gactttttgg ccgttgccgg aaaatgattc atcactaaat ccggcagctc gttgcggata
    24841 tagtcataca ttttgtaatg ttcattccac ggcgcttgcg tcgcgttcag gtaaaacccg
    24901 gcaccttgcc cgagatcgta acggtcggca tctgcgacat gactgcctcg cggactggtg
    24961 tccggcgcaa caacaataat gttgtgctca gccgcgtaac gctgcacccc cgatttagta
    25021 atgaaattct gctcattgca ggtcaggccc gaaagccagt acagcaccgg taatttttca
    25081 cctgcagctt ttggtgggag atagacgccg acattcattt cacatttcag tgatgcggaa
    25141 taatgccgat acacattttg ccagccgcca aagctggcat gtttttcaat gagttccatc
    25201 agtcattatc tcacggttaa tcccccggac ggctgcccgg agagagtatt aagaaccccg
    25261 tctgaagtgg acggggtaaa ccagcgggaa atcagtaacg aattacggtt cgaatggatt
    25321 tgccttcatg catcaggtcg aaggcgtcat taatttcatc caggctcatg gtatgcgtga
    25381 caaacggttc cagatcgata tcacctttca tcgcatcttc aaccataccc ggtaactggg
    25441 aacgaccttt cacgccgcca aacgcggaac ctttccatac gcgaccagtg accaactgga
    25501 atggacgggt ggagatttcc tgaccggaac ccgcgacccc gatgatcacc gactgacccc
    25561 agccgcggtg cgcactttcc agcgccgcac gcatcacgtt gacattaccg atgcattcaa
    25621 aggtatggtc gataccccat ttgttgatat ccagcaggac atctttaatc ggtttgtcgt
    25681 agtcattcgg gttaatgcag tcggtggcac cgaagcgacg cgccagatcg aatttcttcg
    25741 ggttggtatc gatagcgata atccgtcccg ctttcgcctg acgcgcgccc tgaaccactg
    25801 ccagaccaat cgcgccaaga ccaaacacag caacagaatc ccctggctgg actttagctg
    25861 tgttgtgtac cgcgccaata ccggtggtca cgccacagcc cagcaggcag acgtgttcat
    25921 ggtttgcttc tggattaatt tttgccagag acacttccgc gactacggtg tattcactga
    25981 atgtagaaca ccccatgtag tgataaagcg gctgcccatt gtaagaaaaa cgggtggtgc
    26041 cgtctggcat caggccttta ccctgggttt cgcgaaccgc aacacagagg ttagttttgc
    26101 cagaacgaca gaactcacac tcgccgcact ctgcggtgta aagcgggatc acatggtcgc
    26161 ctggtttgac gctggttacg ccttcaccga cttcaaccac aacgccagcc ccttcgtgac
    26221 cgagaaccac cgggaataca ccttccgggt catcgccgga gagggtaaat gcgtcggtat
    26281 ggcaaacgcc ggtatgggtg actttaatca gcacttcacc ttttttcggt ggtgcaacgt
    26341 caatttcaac gatttccagc ggtttaccgg gagcaaatgc aacggcagca cgtgatttca
    26401 tctctcgctc ttcctcaata tggtaataga ttcagctatt taagataggc acgaaccagt
    26461 tcaatagtgt cgtcaacgga ttggctgact tcgcggctgt agcagtcatt tcggtcaaac
    26521 gtttcccgga tatggctttc aagcacttct gccatcagcc cattagccgc gccccgaacg
    26581 gcagcgatct gttggagtat ggcacggcat tcggcatcac cctccagcga ccgttccaga
    26641 gcatcaatct gccctcgaat acgacgaact cgagtaagga cctttttctt ctcttccgga
    26701 gtactgggca tttcgcacct catcatctgc acgcaatata ctataggggg gtatgctata
    26761 tgtcaatgca taccccccta tagtatataa caatggagaa taattgttgt ccgaagccta
    26821 accaggtgaa cggtccctca agacagatgg ggaccgttta tgtgctattt ttatgccgca
    26881 tccgctaaaa caaacatccc gtaaggatgg atttcgaggt aatagtgctc accaatatcg
    26941 ggctgtaaac gggtggcgtt tacttgtaat aaaatctcct gtccatgcca ctctacggtc
    27001 acttcgtact gcggccccat gtaggcgaca tggcgaataa cacaacgctg actctcttcg
    27061 ccgtgattgc ttaaggtgat ggcttccggg cgaacgccga cggtgccgct accttgcacc
    27121 gggaaatgcg ccgcgcgcgg caggcgataa ccgtagatat cgacatactc ttcgctaaag
    27181 ttcgccggga acaggttggc gtcgcccata aaacttgcca taaagcggga ggctggctga
    27241 cgatagagat cctgcggtga gccaatctgc atgatgtgac ctttattcat caccagcaca
    27301 gtgtcggaaa ccgcgaaggc ctcactttga tcgtgagtca cgtagagcga cgtgatatta
    27361 aactgctttt gcagctcgcg gatcttatcg cgcatgctgc ggcgcaggtt ggcatcgagg
    27421 ttacttaacg gctcatcaaa cagcagcacc tttggtttga ggatcaacgt gcgggccagc
    27481 gccacgcgct gttgctgacc accggaaatc tggtcgacat agcggtcctc gaacccttcc
    27541 agatccacca ttgccagcgc ctctttcacc cgctgcttta cttcgctacg cgacacgccg
    27601 agcatcttta acccgtagcc gacgttttcg cccagcgaca tatgcgggaa cagagcgtat
    27661 gactgaaaca ccatgcagat atcgcgctgc tgaatggaac gatgcgtgac atcttcgcca
    27721 tcaataaaga tttgcccttc actcggtttt tccagcccgg caaccagacg caaaatggtg
    27781 gttttgccgc agccggaagg gccaagcagc gtcaccattt gcccttgtgg gatggtgaga
    27841 ttgatattgt cgataaccgt attactgccg aatcgtttag tgacgttgcg cagttccaca
    27901 aaatttttct gactcatagt gcgctccatt acgcctggtt tttggcttta gaacgggaga
    27961 tacgtgcttc tccgatcagc cagtcaaaga ggaaaataat cgccagcatc accacgatca
    28021 gaatcgaacc ataggcgatg gctacgccat attcgccgtc ttcaacgcgg ttaagaatgt
    28081 aggcggttgc cacgcgggtg tccggcgtga cgagaaagac gatggcgctg accgtcgtga
    28141 tggcccgcac aaagctgtag atcagggccg agagaatggc cggacgcagc agcggcagta
    28201 aaatgtgggt tatggttcgc aaggaacccg cgcgcaggct gagtgaggct tcatccagcg
    28261 atttgtcgat ctgccccagg cctgcaatcc cggcgcgaat acccaccgga acgttacgca
    28321 tcaccatcga gataatcaca attgccgccg ttccggtgag ataaaccgga gcgctgttaa
    28381 aggcaagaat atacgaaacg cccgccaccg tacccggtac ggcaaagcac agcatggtgg
    28441 tgaactcgat ggtctttttg cctttgaatt gttgacgcac caccacccag gcgatgagca
    28501 ggccaaagat agcggtaatg ggagcggcaa tcccggcgta gagcagggta tccagcagtg
    28561 aaggccacgc gccgtcgctc attccctgac caaatagctt gataaagtta tccagcgtca
    28621 gggtgtagtc cacgccccag ttcaccgtga agctgccgta gaaaatgctg ccgtagagca
    28681 gggcgttaaa ggcaatccat atcgcaagta tggcgaccac gctccacacc agggtgaccg
    28741 gaagcggctg gacgtcgcca cggtaggatt tgccggaaac cgtgacgtaa gagcgtttac
    28801 caatccacat atactgtatg cagaacacca gcagcgagaa cagcagcagg aaagcgccaa
    28861 gggtgctggc ggcctgataa tccagctgcg agccggtaat atagaagtaa atttgcgtcg
    28921 ccagtacgtc gaagttaccg ccaagtacca gcgggttact gaaatcagcc agtgactgta
    28981 ccacgacgat taaaaaggca ttggcgagcg cgggcttcaa caacggcaca aacacgccgt
    29041 taaaggtttg ccagcggctg gcgcgcaggg tgtaggaggc ctcttccagc gagggatgaa
    29101 tggttttaat ggccccgtcg aggatcataa atgccattgg cgtgaatgcc agaacctgtg
    29161 ccagccagat cccggtaaaa ccgtacagcc agttggtgtt ggtcagccca aaccactcca
    29221 ccataaattc cgtaacatag ccggaacgcc ccatcatgag cgtcacgcct aagccgacca
    29281 caaacggtgg cgtaacgatg ggcaaaatgg aaaagatgcg accaataatg gcgctgcgtt
    29341 tagcgatgcg cgtggtataa atcgccagca ccaggccgaa gaaagcacag cctatcccca
    29401 ccgctatcga caagacgata gagttgacga tcacctgaac aatatgtgcc tgcgagagca
    29461 cggtcataaa cgccagcggg gcgagctctc ccgcgtcgtt ggtgaacatc gggataaaaa
    29521 tggcgatgct cggccagacg ataaaaatgc caatcagcgc cacaatcgca atcagtgagc
    29581 cgatcacgaa gcggtcgccg cccagccatt cgaggcgggt taaggccagc gtcataatcg
    29641 cgcctagttc gacaaacagc acgatggcgg cgtagcctaa accgcgtcca gccacggtgg
    29701 cgctgatgac gataaacgcc atacagagca atgcccagcc ggcatcgaaa taatgacgac
    29761 tacgctgttc gcgcgtggcc tgacgaaacg ggcggaccag caacaggctc gggagtaaat
    29821 accacagcca gctgatatta aaatgcgacc agccgtacgc cccgaggatc tcatcactcg
    29881 tggattccag caggccataa tcgaggctcc agctcggcaa caggacaaat gccagccagc
    29941 ccagcaaaac ccaaaggaaa atcgcatccc gctttttcac gggatggagt gcaagtgtct
    30001 gtgacatagc agttccggta tggttggccg gggcaggcgt ctgcgctgca cccggcaaat
    30061 gcgcctaatg cgggtttatt tacccatctt cacttcgctg acccatttat taatcaatgc
    30121 cttacgaaca tccgtggaac catatttatc catgtcgtag ttgattaatt tcaggtcgtc
    30181 gagcttcagc gaatttggcg acgtttcggc ggtggtatta gtcaggatct gataagactt
    30241 ccctttcttc cacgccagtt cctgagcttc ttttgataat acccagtcca caaacagttt
    30301 ggcgttgtcg aggttgcgcg cgcctttcag aatgctgacg ccgccaattt cgtagcccgt
    30361 accttcacac ggggaaatca actccagcgg cgcgccctgt tctttttcca gcgagtagtc
    30421 atgcaggaag ccaataccaa tcgccgtttc gccacgggcg gcgttacgtg ccggggctat
    30481 gcctgatttg gtgtactgag agacgttggc gttcagttgt tttagataat caaaggcctg
    30541 atcttccccc cacagctgcg caaaagtagc cagggcggtg taggcagtac cggaactttg
    30601 cggatcggca atttgaattt cgcctttata ttccggtttg gtgagatctt tccagcactt
    30661 cggcaccggc aggtttttct ctttcagacg atcggtgttt acgccaaaac cgaggatccc
    30721 cacataaacg gcagaggaca gattcccttt cacttttgcc ggatcgcgga atttctccat
    30781 gatctgctca aggttagggg atttatacgc ctgtagcagg cccatctcgc cagcctgcga
    30841 ttgcgggtct aaagtgccgc cgtaccagac gtcagcctgg gggtttttct tttcagcatc
    30901 gacttttgcc agggtgcttc cggaaccgtt gcggataaac gaggttttta catcatattt
    30961 ctcgccaaac gctttggttt cggcctcaca catttcattg gtggcgctgc aatagaccac
    31021 cagccggcct tttgcctgcg cggcacccgt taaagtcgcc agcgcaacgg cagaagcaat
    31081 cagagtagag gtaaaggtca ttttcattgt tttatccttc gagcgtgagt ggtggtgatg
    31141 cccgccatca gtaacggcat cagcagtaaa cccataagta ctgcggcgac cgagagtaaa
    31201 ctgaacattc ccggccagcc gtagcgttcg atgaccagcg acagcggcca gcctgccagc
    31261 gccgcgccaa ggtaagcgaa caggccgaga aatccggtga ttgaacccgc cgcagctttg
    31321 tgcccacact ccaccgccgc aagtccaatc agcatttgtg ggccaaagac gaaaaagccc
    31381 acggtaaaaa agcacactgc cagcagagcg tagtgatgaa ccggtgccag ccacagtgcg
    31441 gcgactgaca ccatcaaccc caacgtgaac agcaaaatca ttggcgctcg ttgcccgcta
    31501 aacagcaaat ccgatcccca tccggcaaat aacgcgccga gtaagccgcc cacttcaaac
    31561 agcattaccg tggcgttggc gctgagcagg ttgacaccgt ggctttccgt cagccagata
    31621 ttgccccagt cgttaagcgc aatccggatc acataaacca gtacatatga gacgcccagt
    31681 agccagatca gcggattttg cagcatggtg gtgcgcaaca tttgccacag ccccatcggc
    31741 ggactttgtt gttcctgacg taactccagc ggatcgtgac gccagtgacc aactgtcggt
    31801 aatccctctt cctggggcgt gcctttgagt tgtagcgtta accagatccc taatgccatg
    31861 ctgattatcc ccggtgtcag catcgcggcc tgccagcccc accagtgggc ggcaaaggcg
    31921 ctaatcagcg gaataatcgc tccgccaatg ttgatcgaca tattccagca gccccaccag
    31981 aaaccgcgct cattgcgtga gtaccagtgg gtcagcaagc gggcacacgg tggccatccc
    32041 cagccctgaa aaaaaccgtt cagtgtccag accacaagca gaagcgtaag tgattcgcca
    32101 aaggcaaaca ccacgttcaa taacccggtg gcgaacaagc cgacgcccat aaaaccgcgt
    32161 tgtccgtggc tgtcatgcca cagtccggcg gtaaatttcg acaatccgta gctgagataa
    32221 aacagcgaac ccagcaggcc aatgtcgcct ttatccagtc cgaggtcggt ttgtagcgcc
    32281 ggaaggacgt aattcacgct ttttcgcgtc agataaaatg cggcgtagcc aataatcatg
    32341 cacatcagca atcgcggacg taatgtccgg tagcattgat taatttctct cgctgaacgc
    32401 gcgtgcatgg ttgtctccat gttgctgact atatgaaaat gaaagtgaaa ggggatgaga
    32461 gaaaatccgg gatacctaag actttttcct ggttatccgg cgatttgttg caaaaatgtg
    32521 ggcaagttaa caattacccg cgtgccgtgc tgtttttcga gcgttaagtc gccgcccagg
    32581 gcgctgacgc gctcacgcat cccctgaatg ccaaatccag ccattttccc gctggcgatc
    32641 cccacaccgt tgtcgctgac ttccagatgc aatacctctc cctgctggcg caggataatc
    32701 gtcacttcgc tggcttcggc gtgtttacag atgttattga gcaactcctg caataaccga
    32761 tacaaggtaa agcgcaccgt ttcgttttct ggtgtggagt tcagttgata ggcaaactgg
    32821 caatgaatgc cgcgttcact aaaggcaaat tcattaatca gatgcagcaa cgcttcgcga
    32881 aatgtcagtt catcaagcgc gggaggccgc aactggcgca gcaattggcg ggtggagaga
    32941 tgaatgcggc gggcgagctc attgatttgg ctggctgcgg attgaatttg cgccggatca
    33001 cgcgcccgct tcaccagttg cgactggatc tgaatggcgg tgatgttctg accgatttcg
    33061 tcgtgcagct ctcgcgccag gttttttcgc gtgtcctcct cggtgtggat cagcttttcc
    33121 gccaatgccc gccgcgctgc cagctcggcc tccagccgtt gtcggtaatg gtgcaggttt
    33181 agcgccagat gttgctggcg actgatggca atgcccaggc caattcccag caaggcctgg
    33241 gtcgcgagga aaatctccag ctccaccagg ttactaaagc ccacgccaat ctggcgggcg
    33301 atggtgatca tcatgctgcc caaaagcccg gagagtacgc caccttgcca gccaaatttc
    33361 cacgccatca ccacattggg caaaaagacc acgatcaaca gcagacgttc aatttcaggt
    33421 gacagcacca tctgagtgcc gataccaata ataaaaaaca ggctacacca gatgattaaa
    33481 gaggtacgca aggggggatt atgcgtatcg agtcccagga gctggtagcg atgctgctgg
    33541 cgcagaaact caaagataag atagacaaac ggcgtcagta atacgccgcc ggtaaatgag
    33601 gtcaaacccg gtagcattat tgggcctgac ataaaagggg agagcagggc ggtattcaac
    33661 aatgaagctg ccgtgacagt ggctaacagc aacgtcagac gctgccagta aagtggaaaa
    33721 cggtgccaga agttttgcgc aaggagggca ggaaacaggc taataaccgg cgttgccagc
    33781 atgagataac cgttcaatag ctgttcacta tggagccaga acatcatcag caaagggggg
    33841 agcaccaacg caggccagta gcggcgagag agcaaaataa caagggcgag ataaactccc
    33901 tgcgggagaa acaacaccgc ctgttgaccg ttattggtca gataaaagct gagcgtccac
    33961 agcatcagcc agccggttcc ccaggctagc acaataaaca gcaaaatcac ccaatgacga
    34021 agattgcgcg acattaatgc cccgccagca gatggtggtc gagcgcaaaa tgtaccagtt
    34081 caatggtgct atggcagttc agttttccca gcacattggc gcgatgcaca tgtaccgttt
    34141 tatgactgag ttcgagttta aaggcgatct ctttaacgct gtctcctttg accagtaact
    34201 caaacacttc acgttctcgt ggggtgagaa tttccagcgc ctgggcgggt tgcccgccgc
    34261 cgcgtaaagc ccgaatcgca tcggcacaga gataatgccc gcccagcccg accgagcgca
    34321 ccgcctgtac taactcttcc ggcccacagc gtttggttaa atacccgctg gcaccggcat
    34381 ccagcgcgct ttgcacaaag gttggcgcgt cataaatgct aagaataatg gcgcgaaatt
    34441 gcggtttttg tgcacgcagg cgcttaagca aactgaggcc gttttcgtcc ggcatggcaa
    34501 tatcaatgac ggcaacattg acgtcatcac ggattaatgc cgaccatgcc tgcgcggcac
    34561 tactgtactg accaatgacc tccagatcgt cttccagaga gagtaattgt gcgaagccag
    34621 agcgcaccac aacatggtca tccaccagca ctacccgaat catacttttc ttctcttatt
    34681 atctaattct cctatcatgc atcacggcgc ggcgctggca atcaaccagc gacagatatg
    34741 gaatggatgt aacaaaatgg caataatttg ccggagcgcg cacatcttat ctggcttgtg
    34801 aaccggcggg atacgttcgc caaaaccgca aaatgcgcaa actcgctaac cattgccgat
    34861 ttttttctaa aaacgctttg ttcttaacaa gaccttttaa ttcctttatc aaactgaccc
    34921 gcaacgaaat actgctccca taacaagaca ggggagcaga caatcatggc aatttcatcg
    34981 cgtaacactc ttcttgccgc actggcattc atcgcttttc aggcgcaggc ggtgaacgtc
    35041 accgtggcgt atcaaacctc agccgaaccg gcgaaagtgg ctcaggccga taacaccttt
    35101 gctaaagaaa gtggagcaac tgtggactgg cgcaagtttg acagcggagc cagcatcgtg
    35161 cgggcgctgg cttctggcga cgtgcaaatc ggcaaccttg gttccagccc gttagcggtt
    35221 gcagccagcc aacaggtgcc gattgaagtc ttcttgctgg cgtcaaaact gggtaactcc
    35281 gaagcgctgg tggtaaagaa aactatcagc aaaccggaag atctgattgg caaacgcatc
    35341 gccgtaccgt ttatctccac cacccactac agcctgctgg cggcgctgaa acactggggc
    35401 attaaacccg ggcaagtgga gattgtgaac ctgcagccgc ccgcgattat cgctgcctgg
    35461 cagcggggag atattgatgg tgcttatgtc tgggcaccgg cggttaacgc cctggaaaaa
    35521 gacggcaagg tgttgaccga ttctgaacag gtcgggcagt ggggcgcgcc aacgctggac
    35581 gtttgggtgg tgcgcaaaga ttttgccgag aaacatcctg aggtcgtgaa agcgttctct
    35641 aaaagcgcca tcgatgctca gcaaccgtac attgctaacc cagacgtgtg gctgaaacag
    35701 ccggaaaaca tcagcaaact ggcgcgttta agcggcgtgc ctgaaggtga cgttccgggg
    35761 ctggtgaagg ggaataccta tctgacgccg cagcaacaaa cggcagaact gaccggaccg
    35821 gtgaacaaag cgatcatcga caccgcgcag tttttgaaag agcagggcaa ggtcccggct
    35881 gtagcgaatg attacagcca gtacgttacc tcgcgcttcg tgcaataaaa ggaggcgcag
    35941 atgctgcaaa tctctcatct ttacgccgat tatggcggca aaccggtact ggaagatatc
    36001 aacctgacgc tggaaagcgg cgagctactg gtggtgctgg ggccgtccgg ctgcggtaaa
    36061 accaccctgc tgaatttgat tgccggtttt gtgccttatc agcatggcag cattcaactg
    36121 gcgggtaagc gtattgaggg gccgggagca gagcgtggcg tagtttttca gaatgaaggg
    36181 ctactaccgt ggcgcaatgt acaggacaac gtggcgttcg gcctgcaatt ggcaggtata
    36241 gagaaaatgc agcgactgga aatcgcgcac cagatgctga aaaaagtggg gctggaaggc
    36301 gcagaaaaac gctacatctg gcagctttcc ggtggtcaac gtcagcgggt ggggattgcc
    36361 cgcgcgctgg cggcgaatcc ccagctgtta ttactcgacg aaccgtttgg tgcgctggac
    36421 gccttcaccc gcgaccagat gcaaaccctg ctgctgaaac tctggcagga gacgggcaag
    36481 caggtgctgt tgattaccca cgatatagaa gaagcggtgt ttatggcgac tgaactggtt
    36541 ctgctttcac ccggccctgg ccgcgtgctg gagcggctgc cgctcaactt tgctcgccgc
    36601 tttgttgcgg gagagtcgag ccgcagcatc aagtccgatc cacaattcat cgccatgcgc
    36661 gaatatgttt taagccgcgt atttgagcaa cgggaggcgt tctcatgagt gtgctcatta
    36721 atgaaaaact gcattcgcgg cggctgaaat ggcgctggcc gctctcgcgt caggtgacct
    36781 taagcattgg cacgttagcg gttttactca ctgtatggtg gacggtggcg gcgctgcaac
    36841 tgattagccc gctgtttttg ccgccgccgc aacaagtact ggcaaaactg ctcaccattg
    36901 ccggaccgca gggatttatg gacgccacgc tgtggcagca tctggcagcc agcctgacgc
    36961 gcattgtgct ggcgctactc gcggcggtgt tgatcggcat tccggtcggg atcgccatgg
    37021 ggctaagccc gacggtgcgc ggcattctgg atccgataat cgagctttat cgtccggtgc
    37081 cgccgctggc ttatttgccg ctgatggtga tctggtttgg tattggtgaa acctcgaaga
    37141 tcttactgat ctatttagcg atttttgcac cggtggcgat gtcggcgctg gcgggggtga
    37201 aaagcgcgca gcaggttcgc attcgtgctg cccagtcgct gggtgccagc cgtgcgcagg
    37261 tgctgtggtt tgtcattttg cccggcgcgc tgccggaaat cctcaccgga ttacgtattg
    37321 gtctgggtgt gggctggtct acgctggtgg cggcggagct gattgccgcg acgcgcggtt
    37381 taggatttat ggttcagtca gcgggtgaat ttctcgcgac tgacgtggtg ctggcgggga
    37441 tcgcggtgat tgcgattatc gcctttcttt tagaactggg tctgcgcgcg ttacagcgcc
    37501 gcctgacgcc ctggcatgga gaagtacaat gagtgaacgt ctgagcatta ccccgctggg
    37561 gccgtatatc ggagcacaaa tttcgggtgc cgacctgacg cgcccgttaa gcgataatca
    37621 gtttgaacaa ctttaccatg cggtactgcg ccatcaggtg gtgtttctgc gcgatcaagc
    37681 cattacgccg cagcagcaac gcgcgctggc ccagcgtttt ggcgaactgc atattcaccc
    37741 tgtttaccct catgccgaag gggttgacga gatcatcgtg ctggataccc ataacgataa
    37801 tccgccagat aacgacaact ggcataccga tgtgacattt attgaaacgc cacccgcagg
    37861 ggcgattctg gcagcgaaag agttaccttc gaccggcggc gatacgctct ggaccagcgg
    37921 tattgcggcc tatgaggcgc tctctgttcc cttccgccag ctgctgagcg ggctgcgtgc
    37981 ggagcatgat tttcgtaaat cgttcccgga atacaaatac cgcaaaaccg aagaggaaca
    38041 tcaacgctgg cgcgaggcgg ttgcgaaaaa tccgccgttg ctacatccgg tggtgcgaac
    38101 gcatccggtg agcggtaaac aggcgctgtt tgtgaatgaa ggctttacta cgcgaattgt
    38161 tgatgtcagc gagaaagaga gcgaagcctt gttaggcttt ttattcgccc atatcactaa
    38221 accggagttt caggtgcgct ggcgctggca accgaatgat attgcgattt gggataaccg
    38281 tgtgactcag cactatgcca atgccgatta cctgccacag cggcggatta tgcatcgggc
    38341 gacgattctt ggggataaac cgttttatcg ggcggggtaa tacgagagtg gacggtcctc
    38401 tcgccccctt ggggagaggg ttagggtgag ggggcgttca ccgtgctttc aacaggttaa
    38461 ctcccccttt ctgagaggaa acaaaattaa cgcagaatct tcttctcagc caaatccagc
    38521 gcaaagtagc tgaaaatcag atccgcaccc gcacgcttaa tcgaacctaa gctttcgagc
    38581 acgactttct cttcatctat agcacccgcc agcgcggcga acttaatcat cgcatactca
    38641 ccgctcacct gatacgcgcc aatcggcaat tcagtacgtt cacgcagctc acgcacgatg
    38701 tcgaggtacg ctccagcagg tttaaccatc aggcagtctg cgccctgggc ttcatccagc
    38761 agtgactcac gaatcgcctc acgacggttc attgggttca tctgatagct tttgcggtcg
    38821 ccttttaatg cgcttccggc agcttcacgg aacggaccat aaaaggagga ggcgaacttg
    38881 gtcgaatacg acataatcgc cgtatcttta aagccagcag cgtccagcgc ctggcgaatc
    38941 gcctgtacct ggccatccat cgcggcggaa ggggcgatga agtctgcacc tgcagcagct
    39001 gcaaccacgg cttgcttgcc taaattttcc agagtcgcgt cgttgtcgac gccatgctcg
    39061 cacagcacac cgcagtgacc gtgagaagtg tattcacaga agcaggtgtc tgacatgaca
    39121 atcatttctg gcaccgtctg cttgcagatg cgcgacatac gcgctaccag tccatcttcc
    39181 cgccaggcat cgctgccggt ttcatcggta tggtgagaga tgccgaaagt catcacggaa
    39241 cgaataccgg cgttggcgat gcgttcaatt tcgcgtgcca gatgtttctc tggaatgcgc
    39301 atcacgccag gcatggcttc aacggctttg tagtcgtcaa tttcttcttc aacaaagatc
    39361 ggcaacacca ggtcgttaag gctaagtgtt gtctcttcaa acatagcgcg cagcgcagga
    39421 gatttgcgca ggcgacgagg gcgttggatt aagtctgtca tggtctgcct gatgtttgtg
    39481 gaattaaggt gcacagtata cctgaagcgg ggctggagtg ttttacgaaa gttgtcacga
    39541 tgttgacaag aagagaatga aagagagacc agggcatctt tgtaatagaa atagcaataa
    39601 cttcatattc act
//
LOCUS       DAZFDC010000026        39349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFDC010000026 DAZFDC010000000
VERSION     DAZFDC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.026"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            204..1859
                     /gene="aslA"
                     /locus_tag="ACHJ48_001530"
     CDS             204..1859
                     /gene="aslA"
                     /locus_tag="ACHJ48_001530"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO4707116.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKEPQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(2027..3262)
                     /gene="aslB"
                     /locus_tag="ACHJ48_001531"
     CDS             complement(2027..3262)
                     /gene="aslB"
                     /locus_tag="ACHJ48_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4707117.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETTAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(3409..3485)
                     /locus_tag="ACHJ48_001532"
     tRNA            complement(3409..3485)
                     /locus_tag="ACHJ48_001532"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3449..3451),aa:Pro,seq:tgg)
     gene            complement(3528..3614)
                     /locus_tag="ACHJ48_001533"
     tRNA            complement(3528..3614)
                     /locus_tag="ACHJ48_001533"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3578..3580),aa:Leu,seq:cag)
     gene            complement(3635..3710)
                     /locus_tag="ACHJ48_001534"
     tRNA            complement(3635..3710)
                     /locus_tag="ACHJ48_001534"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3675..3677),aa:His,seq:gtg)
     gene            complement(3769..3845)
                     /locus_tag="ACHJ48_001535"
     tRNA            complement(3769..3845)
                     /locus_tag="ACHJ48_001535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3809..3811),aa:Arg,seq:ccg)
     gene            complement(3948..5333)
                     /gene="yifK"
                     /locus_tag="ACHJ48_001536"
     CDS             complement(3948..5333)
                     /gene="yifK"
                     /locus_tag="ACHJ48_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4707118.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5524..6264)
                     /gene="wecG"
                     /locus_tag="ACHJ48_001537"
     CDS             complement(5524..6264)
                     /gene="wecG"
                     /locus_tag="ACHJ48_001537"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO4707119.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEENAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTTVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6267..7619)
                     /gene="wzyE"
                     /locus_tag="ACHJ48_001538"
     CDS             complement(6267..7619)
                     /gene="wzyE"
                     /locus_tag="ACHJ48_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO4707120.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7616..8695)
                     /gene="rffT"
                     /locus_tag="ACHJ48_001539"
                     /gene_synonym="wecF"
     CDS             complement(7616..8695)
                     /gene="rffT"
                     /locus_tag="ACHJ48_001539"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO4707121.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDASLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPHNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8692..9942)
                     /gene="wzxE"
                     /locus_tag="ACHJ48_001540"
     CDS             complement(8692..9942)
                     /gene="wzxE"
                     /locus_tag="ACHJ48_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO4707122.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISLGLFGNHDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMTLITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPTHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9944..11074)
                     /gene="rffA"
                     /locus_tag="ACHJ48_001541"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9944..11074)
                     /gene="rffA"
                     /locus_tag="ACHJ48_001541"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO4707123.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCLQNAHMFYIKLRDIGDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11079..11753)
                     /gene="rffC"
                     /locus_tag="ACHJ48_001542"
                     /gene_synonym="wecD"
     CDS             complement(11079..11753)
                     /gene="rffC"
                     /locus_tag="ACHJ48_001542"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO4707124.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPFLTPDALAPWSRV
                     QAKIAASNTGELDALQQQGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11731..12612)
                     /gene="rfbA"
                     /locus_tag="ACHJ48_001543"
     CDS             complement(11731..12612)
                     /gene="rfbA"
                     /locus_tag="ACHJ48_001543"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO4707125.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEVATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12631..13698)
                     /gene="rffG"
                     /locus_tag="ACHJ48_001544"
     CDS             complement(12631..13698)
                     /gene="rffG"
                     /locus_tag="ACHJ48_001544"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO4707126.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPQGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWRKQVQDGSYQGERLGLKG"
     gene            complement(13698..14960)
                     /gene="wecC"
                     /locus_tag="ACHJ48_001545"
     CDS             complement(13698..14960)
                     /gene="wecC"
                     /locus_tag="ACHJ48_001545"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO4707127.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14957..16087)
                     /gene="wecB"
                     /locus_tag="ACHJ48_001546"
     CDS             complement(14957..16087)
                     /gene="wecB"
                     /locus_tag="ACHJ48_001546"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO4707128.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16143..17189)
                     /gene="wzzE"
                     /locus_tag="ACHJ48_001547"
     CDS             complement(16143..17189)
                     /gene="wzzE"
                     /locus_tag="ACHJ48_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO4707129.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17201..18304)
                     /gene="wecA"
                     /locus_tag="ACHJ48_001548"
     CDS             complement(17201..18304)
                     /gene="wecA"
                     /locus_tag="ACHJ48_001548"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO4707130.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGNLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFLLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18544..19803)
                     /gene="rho"
                     /locus_tag="ACHJ48_001549"
     CDS             complement(18544..19803)
                     /gene="rho"
                     /locus_tag="ACHJ48_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO4707131.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19888..19989)
                     /gene="rhoL"
                     /locus_tag="ACHJ48_001550"
     CDS             complement(19888..19989)
                     /gene="rhoL"
                     /locus_tag="ACHJ48_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO4707132.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20130..20459)
                     /gene="trxA"
                     /locus_tag="ACHJ48_001551"
     CDS             complement(20130..20459)
                     /gene="trxA"
                     /locus_tag="ACHJ48_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO4707133.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20590..21855
                     /gene="rhlB"
                     /locus_tag="ACHJ48_001552"
     CDS             20590..21855
                     /gene="rhlB"
                     /locus_tag="ACHJ48_001552"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO4707134.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHCLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21991..23475
                     /gene="gppA"
                     /locus_tag="ACHJ48_001553"
     CDS             21991..23475
                     /gene="gppA"
                     /locus_tag="ACHJ48_001553"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO4707135.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(23522..25543)
                     /gene="rep"
                     /locus_tag="ACHJ48_001554"
     CDS             complement(23522..25543)
                     /gene="rep"
                     /locus_tag="ACHJ48_001554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO4707136.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25760..26209
                     /locus_tag="ACHJ48_001555"
     CDS             25760..26209
                     /locus_tag="ACHJ48_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO4707137.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNAFIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26408..26689
                     /gene="ppiC"
                     /locus_tag="ACHJ48_001556"
     CDS             26408..26689
                     /gene="ppiC"
                     /locus_tag="ACHJ48_001556"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO4707138.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26735..28210)
                     /gene="ilvC"
                     /locus_tag="ACHJ48_001557"
     CDS             complement(26735..28210)
                     /gene="ilvC"
                     /locus_tag="ACHJ48_001557"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO4707139.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVHTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDLNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28360..29253
                     /gene="ilvY"
                     /locus_tag="ACHJ48_001558"
     CDS             28360..29253
                     /gene="ilvY"
                     /locus_tag="ACHJ48_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO4707140.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(29305..30849)
                     /gene="ilvA"
                     /locus_tag="ACHJ48_001559"
     CDS             complement(29305..30849)
                     /gene="ilvA"
                     /locus_tag="ACHJ48_001559"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO4707141.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30852..32702)
                     /gene="ilvD"
                     /locus_tag="ACHJ48_001560"
     CDS             complement(30852..32702)
                     /gene="ilvD"
                     /locus_tag="ACHJ48_001560"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO4707142.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQISFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32767..33696)
                     /gene="ilvE"
                     /locus_tag="ACHJ48_001561"
     CDS             complement(32767..33696)
                     /gene="ilvE"
                     /locus_tag="ACHJ48_001561"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO4707143.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33716..33979)
                     /gene="ilvM"
                     /locus_tag="ACHJ48_001562"
     CDS             complement(33716..33979)
                     /gene="ilvM"
                     /locus_tag="ACHJ48_001562"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO4707144.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33976..35622)
                     /gene="ilvG"
                     /locus_tag="ACHJ48_001563"
     CDS             complement(33976..35622)
                     /gene="ilvG"
                     /locus_tag="ACHJ48_001563"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO4707145.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLKLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35625..35675)
                     /gene="ilvX"
                     /locus_tag="ACHJ48_001564"
     CDS             complement(35625..35675)
                     /gene="ilvX"
                     /locus_tag="ACHJ48_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO4707146.1"
                     /translation="MNNSTKFCFPRFRTGN"
     gene            complement(35762..35860)
                     /gene="ilvL"
                     /locus_tag="ACHJ48_001565"
     CDS             complement(35762..35860)
                     /gene="ilvL"
                     /locus_tag="ACHJ48_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO4707147.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            36213..37733
                     /gene="yifB"
                     /locus_tag="ACHJ48_001566"
     CDS             36213..37733
                     /gene="yifB"
                     /locus_tag="ACHJ48_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO4707148.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(37758..38096)
                     /gene="maoP"
                     /locus_tag="ACHJ48_001567"
     CDS             complement(37758..38096)
                     /gene="maoP"
                     /locus_tag="ACHJ48_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO4707149.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            38215..39054
                     /gene="hdfR"
                     /locus_tag="ACHJ48_001568"
     CDS             38215..39054
                     /gene="hdfR"
                     /locus_tag="ACHJ48_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO4707150.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(39150..39225)
                     /locus_tag="ACHJ48_001569"
     tRNA            complement(39150..39225)
                     /locus_tag="ACHJ48_001569"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39190..39192),aa:Trp,seq:cca)
     gene            complement(39234..39310)
                     /locus_tag="ACHJ48_001570"
     tRNA            complement(39234..39310)
                     /locus_tag="ACHJ48_001570"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39274..39276),aa:Asp,seq:gtc)
BASE COUNT         9629 a        10711 c         9914 g         9095 t
ORIGIN      
        1 tataataaac ctgccttaca tatcaactgc gccagaggta ggattgaaaa cgctctcctg
       61 atttttaaat tcatttcttg gataaataaa taatttattt ttatcactat tatttatgta
      121 atcatcctgt cagggagagg gatctcaatt atcaatgctt aattacgtca tcattttgtt
      181 gacatgaaac cggagcattt tgcatggaat tttcgttttc acccaaactt cttgttgttg
      241 ctgtcgccgc cgctcttcct ctcatggcca gcgcagcaga taccccgtca actgccaccg
      301 cgcgcaaagg ctttgccgga tacgatcacc caaaccagta tctggttaaa ccggcgacca
      361 ctattgccga caacatgatg ccagtaatgc agcatccggc gcaggataaa gaaccccagc
      421 agaagctggc agaacttgag aaaaaaaccg gtaagaaacc gaatgtggtt gttttcttgc
      481 tggacgatgt gggctggatg gacgtcggtt ttaacggtgg cggcgtggcg gtgggtaacc
      541 ctacaccaga tatcgacgcc gttgccagcc aggggctgat tttaacttcg gcgtattctc
      601 aaccaagctc ttccccaacc cgcgccacca ttctcaccgg acaatactcc atccaccacg
      661 gcattctgat gccgccaatg tacgggcaac cgggcgggct gcaagggtta accacgctgc
      721 cgcagttgct gcacgatcag ggctacgtca ctcaggccat cgggaaatgg catatggggg
      781 aaaacaaaga gtcgcagccg cagaacgttg gctttgatga tttccgtggc tttaactcgg
      841 tgtctgatat gtacaccgaa tggcgcgacg ttcacgtcaa tccggaagtg gccctgagtc
      901 cggaccgttc tgaatacatc aagcaattac catttagcaa agatgacgtt catgcggtgc
      961 gcggcggcga acaacaggcc attgccgaca ttacgccgaa atatatggaa gatctggatc
     1021 aacgctggat ggaatatggc gttaagttcc tcgacaagat ggcgaagagt gataagcctt
     1081 tcttcctcta ttacggcact cgcggctgtc acttcgataa ctacccgaac gccaaatatg
     1141 cgggtagctc tccggcacgc acctcgtatg gcgactgcat ggtggaaatg aacgatgtgt
     1201 tcgctaatct gtataaagca ctggagaaaa acggtcagct tgataacacg ctgattgtct
     1261 ttacctccga taacggaccg gaagccgaag taccgccgca cggacgcaca ccgttccgtg
     1321 gtgcgaaagg atctacctgg gaaggcggcg ttcgcgtacc gactttcgtt tactggaaag
     1381 gcatgatcca accgcgtaaa tctgacggta ttgttgatct ggcagatctc ttcccaacag
     1441 cactggatct ggcggggcat cctggtgcga aagtggcgaa tttagtgccg aaaaccacct
     1501 ttatcgatgg tgtggaccag acatctttct tcctgggaac aaatggtcag tctaaccgta
     1561 aggccgagca ctacttcctc aacggtaaac tcgctgctgt gcgtatggat gaattcaagt
     1621 atcacgtcct gattcagcaa ccttacgctt atacccagag cggatatcag ggtggattca
     1681 ccggcacagt aatgcaaaca gccggatcgt cggtgtttaa cctctacacc gatccgcagg
     1741 agagcgactc tatcggcgtg cgccatattc cgatgggtgt accgctacag accgaaatgc
     1801 acgcgtatat ggagatcctg aaaaagtatc caccacgcgc gcagattaaa tctgattaag
     1861 ccggcgctat cgctgagatc tgcctttgcc ggatgcgatg ctgacgcatc ttatccagcc
     1921 tacagaacgc tgcaatttat tgaatttgca cgatcatgta ggccggataa ggcgtttacg
     1981 ccgcatccgg caatcaaccg caggcgacca caaaatggcc gcttttttac ttactcacca
     2041 ccagcaaatg cgcctgcata atgtcgctgg ccgggcgacc gtgcgccagc aaatcagcca
     2101 ttgctttaag atatggcggt agatggcgga aataacgctg atacccggca cacaaataat
     2161 tcagtcccgg tttgccgctg gcatcgagca tgaagcggtg tttcgggcag cctccccaac
     2221 atgcttttaa cacgttacaa ctgcgacact gcgccggtaa ttgcttaaat ttatcttcac
     2281 caaacacctg ctgttgcggg gaatcgatca tttctgcaat tgtttgctgg tgcatattcc
     2341 ccagtcgata ttgcggataa acatagtgat cgcaggcgta aacgtcgcca ttgtgctcaa
     2401 caatcaccga gcgcccacag gttggctgat gatggcaaac cgcacccggc gcaccgacaa
     2461 aattggcaaa cgcccattcg atattcatca cgaaaatctt gccgacgtcg cgtttaatcc
     2521 agtggtcgaa tatcgccacc agaaattcac caaattcatc ggggcgcaca gaccattccg
     2581 tcagttcccc ctgaatatca ccaggcgcat gtagtttcag tccttcgcgg gcagtcgttt
     2641 catcagccag gcgctcgacc accggaataa actggatgaa ttcgactccc gcatcgcaca
     2701 aaaaatcata aacctgtaac ggttgctgcg cgctggtgcg gttgacgcag accagcacgt
     2761 tatagtcgac atgatgtttt tgcaggagcg tcagggcacg catcaccagt ttatgcgtgg
     2821 gtctgccgcc ttttgtcacg cgatattgat tgtggatctc agccggacca tccagcgata
     2881 acccaacaag aaaatgattt tctgccagaa atgcacacca ttcatcatcg agcagtacgc
     2941 cgttagtctg gaagctgtta cttatcttcc tgccagcacc atatttcgcc tgtagcgcca
     3001 cggcacggcg gtaaaactcc agcccgagta gcgttggttc gccgccctgc caggtaaaag
     3061 ccacttcgtt ttgcggttcg ctggcagcga tatagtgacg gacatacgct tccagcgtgt
     3121 catcgtccat atgcgtgact ggcttttcgc ggtaaaggga ttgtttttcg agataaaaac
     3181 agtagtcaca gttcagatta caatcggaac cgctcggttt cgccatcaca tgaaaagcac
     3241 gcgttggaac ctgttgcagc atggtcgctc cttgcctgaa gtcatccttc aatacgttaa
     3301 gggcgaaatc ataccgtggg gcaatgagtt attacgggcg tttattgcga agggagggtg
     3361 acgaaatgca cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga
     3421 gaggattcga acctccgacc cactggtccc aaaccagttg cgctaccaag ctgcgctact
     3481 cgccgaaata ctgctttttg aatttttagt tcaattcttt aaagtcgtgg tgcgaggggg
     3541 gggacttgaa cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat
     3601 tccgccacct tcgcattgtc acaacttcta ataatggggt ggctaatggg attcgaaccc
     3661 acgacaactg gaatcacaat ccagggctct accaactgag ctatagccac cactacaaat
     3721 cttgttacgc ggtattacta ccaccgcagc tcaagcgccg ggactaaatg gcgcgcccga
     3781 caggattcga acctgagacc tctgcctccg gagggcagcg ctctatccag ctgagctacg
     3841 ggcgcttagc gccgttgcgg gggtggataa tacggacttc ccccccctct gtctagtccc
     3901 tttttaaata aattgcgcgt ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt
     3961 ttatgcgctt tcccgtggcg attaaggcca aaaactttat aaatcgccgt caccgccagc
     4021 ataaagatga tgccaacaaa cagcgacata cgcgtatctt cattaaagta catgccgatc
     4081 aaaacgcaaa tcaggaatgc cattgttacg tagttggccc acgggaacag gattgagcgg
     4141 aacggatggc tggcaatcgc cgctttatgt gcacgccgaa aacgcagctg gcttatcaat
     4201 atcacaaacc atggcaccat ccccggaagc acgctggcac tgtagacgta gacaaacaca
     4261 cgctgcggat tgggaatgat gtagttcagg catgagccaa ttagcagaat agcaatagat
     4321 actgccacac ccgcaaccgg tacgccgtga cgggaaactt tcgccatcgc cgccggtaac
     4381 tgacggtttt tcgccagtgc gtagagcata cgtccgcaac tgtacatgcc gctgttacag
     4441 ccagagagcg cagccgtcag caccacaaag ttgataatgc ccgccgctgc ggtaataccg
     4501 attttggcaa aagtcagtac gaacgggctg ccgttgctgc ctatttcatt ccacgggaag
     4561 atggtgacga taacgaaaat cgcgccaacg tagaaaatca ggatccgcca cagcaccttg
     4621 cctacggcac tgcgcagcgt cacctgcgga ttcttcgctt caccggcagt aatgccaatc
     4681 agctccacgc cctggtagga cgccaccaca atacacagcg cggtcaggaa ccctttccag
     4741 ccacccgcaa agaaaccgcc atgctctgtg agattgctaa aaccaatcga ctgcccgcca
     4801 ttgccaaagc caaagaaaat cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg
     4861 actttgatca tcgcgaacca gaactcgatt tcgccgtaca accgcaccgc tgccagattc
     4921 gccaacgcca ccagcgccac tgcgatcaat gcgggtatcc actgcgccat ctccgggaac
     4981 cagaactgga cataaacgcc aatggcggtg atttcagaga tccccaccgc catccacata
     5041 aaccagtaag accaggcggt gagatagcca aagaacgggc tcatataacg atgcgcataa
     5101 acggcgaacg aaccggtaac cggttcgagg aacaacattt cgcccattga acgcatgatg
     5161 aaaaagacga acagcccggc gatgatatag gccaacaata cggatggccc ggcccatttc
     5221 agggtactgg cggcccccat aaacaggccg acgccaatgg tgccccccag ggcgatgagt
     5281 tcgatatgtc gagcttccag cccacgctgt agctctggtt tgttatctgc cataaatcct
     5341 cgtgttgtgt ttgcatgctt tccggtgtta ccggttatcg ttatgggtac atcgagtgtt
     5401 gcgaatgttt tcgtaattca ggagaaatgg caaataaagc attaaaaatt tgaatgcttt
     5461 gtgtaataaa aaagcagaca ggcgacggag tgaccactcc gtcgctttac aaagagagga
     5521 aaatcagagg ttgccggtgt aatgccagcg taaataacgc agcaaacgaa gctgacgctt
     5581 aatgcggctc ggctgcgaaa gcaggcggta gagccactcc agccccagcg tttgccagat
     5641 tttcggtgcg cgttttacgt gaccggtgaa aacatcgtaa gtcccgccaa cgcccatata
     5701 cagcgcatct ggatgcacca gacggcagtc gcgcatgaag atctcctgct ttggcgatcc
     5761 catcgcaacg gtgacgattt gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg
     5821 ctgctcgggt ttaaaataac catcctgact gccaacgata ttcacattcc actggttgcg
     5881 cagtttagct tcagtttgcg ccagcacttc aggtttaccg cccacaagaa ataccgtcgt
     5941 cccttctttg cctgcgcgcg ccatcagctc ttcccagaga tcggcaccgg caacgcggga
     6001 aacctgcgcc tgcgggtact ttttacgtac tgaacgtaca acgctgatgc catccgcata
     6061 tttaaattcg gcagcgttaa ttaactccct gacctcggcg ttctcttcaa tggtcagcat
     6121 tttttcagca ttaatggcaa ccagcgttcc ctgcttaagc tgcccgtcag caaacagata
     6181 atcgagggca tgctgcatat cacgccaacc aatcaactgt aagccacgca gcgtataggt
     6241 cggtgccgtg gtgttgttat tcattgttat ccttcaacct gcgtccggag cgatgatttt
     6301 gtacgtttat gaatgagtcc ggcgctttca aaaagccagt acaacagttt tgcgatcatc
     6361 agacatgcgc cgaagaccac gataaaaaag accacgcgtg agacaaacga atccagccct
     6421 tcacgcgcca gcacgatcat attgaagatc gccccaaagc agaaactgtg caatatcgca
     6481 gccttatagc gattagtttc gcggttgccc agctcataca gccagtcgaa ccatttgatg
     6541 atcagtccaa ccacaatcgc cccgagcggg atgaacaacg cgccgcccat caccaccagt
     6601 gagcctataa gcgtaggcga gatcgccagt ccggagtggt tattcagcac ttcccaggta
     6661 aagtagttgg ctgagttcag caccatactc gggcgacccg gccacagcca ggaagggata
     6721 aagacataga aatcgcggac aattggcgcc aggccctgga agtcgatgtt gtcgtagttc
     6781 tgcaacagca acgccagatt ctcccacggc gagaaggtgt cgcgagtgag atagagaaac
     6841 gtatagaacg cttcatcgcc gctcacattc attccatagc gttttagcgc cagccagaac
     6901 atgccaacaa tccccagcac gcccgccgcc gccagcatcc acaacgaaat ccagccgcga
     6961 ataatgccaa taaacaggaa gatagcgaat gcgatgatga tattggcgcg agtgccgccg
     7021 acaatcatat aagtcagcaa gccaaaggcg accgtgctga cgaggaaaaa cagccacgct
     7081 ttgctgtcct ggcgcagaaa gtagaccacc agcatcgccg ggatgaaaaa gtaaaagaag
     7141 cgttttaacg ccacgccgga gacttcactg gaaaagatct gactgtagga gttaagccgg
     7201 aacagcaaaa agccgttgtg catgaagaag atgccgacgc ttaccagcgc gatacccatc
     7261 aggatcaccc acgtaagatt ggtttccacg cggttcatgg taaacagcgg acggcgcggt
     7321 acatcagcaa cgcgtttgcg taggcgggtt ttgtaggtga cgtagtaaac cgcgtagaag
     7381 cagcccgcag aaagcaacgc ctgcaacagg atttctggcg gcgcgacacc aacatcaaag
     7441 cgaaacacca gcacgctggt cagcgggaag ccaaagaaaa aggtgagcag aaacagcaat
     7501 gaaaagaata cattgaagtt aaagcgcaca cgacgaaact caaaccaggt cagcgtggca
     7561 ataaacagcg tgcagagcag ccagacaaca aacaggccac tgaattgcag cagactcatg
     7621 cgacctcccc ggcggcaatc gccaacgccc gctgccagcc ttgtagatag ttagggctaa
     7681 agaaggcaat ggtgttttta tccaccgacg ccaactggcg ctgcgcttca cgcacaatat
     7741 cctcgttgag atcgtcggta gtaaacagca ccggtaaatg ctgttccgtc atatcctgcc
     7801 agaacggatt ttcccggtta agcacacaag gaatgcccgc ctgaatcagt aagcacagcg
     7861 taccaatgcc ctgctggcgg gcaaaaataa agtaaccaag atcgcactga cgaagtagcg
     7921 tcagataggc gtcaaattcc agtttttcac tcagaacttg cagattttct tcgctgaata
     7981 actccagccc cgcctgacga acttcctcga tgtacgcttc gttatgaggc ggatatccca
     8041 tcggcaccac cacttttacc gtatcgccaa attgctgatg aacggcgcgc aaggcagcaa
     8101 tatgctcatt gctgcggtcg ccggagttcc ccaccagaat ggtcattttc ccttcacgtt
     8161 gccgatcgtt cgccatcgta ttgagagaag catccatccg cgtcgggaag tacagcagtt
     8221 cgccccgcac ctttgggtgc gttttggcaa aaaagctcaa atcaccacgg gtggcaaata
     8281 cacagccgac tcgcttttgc gccaggcgac gtagtgggta aaaaagctta tatctcaagc
     8341 cactggaaag ctcgtacagg tctgcccccc agatatgcca gtaaaactgg ctgggcttaa
     8401 tgccaccact cagcagagcc agccacagtg tgggattgaa ctgaccgtgg aagaaaaaac
     8461 gctgctgacg gttagctttt gctttcgcga tgaccgcttc cgccagcgat tttttcccag
     8521 ggaaaaattg cacagaaagc gccggacagc tatcacttaa gccgtcgtcc ttgccaacaa
     8581 ccataaactc gcgcgcgtgc tcgctcgtcg cggccagcgc gtcattgaaa aaccgcaaaa
     8641 cggttcggtt atggtgaggg atatccgatc ccagtacgtg aatcagtaca gtcatgcccg
     8701 cctacgccag agtaaaaaca cgccacaaca aagagaaaaa tagacgatat aggttgccat
     8761 atatgcctgt gccgcgccca gcgcgccgtg cgtagggatt agccagtggg caaataccat
     8821 caataaagtg aactggctga cttccgccag aatataaaac cgcagtgaag ctttggcgat
     8881 caccagataa ccaaagacat aagcgcccac ttttaacaca tcacccacca actgccaggc
     8941 aaagagatcg cgcatagcgg taaatttatt cgacaacagc agccagatag caaaatcacg
     9001 cagcaaccag acggtaaaac tcgccgccgc cactgccggt aagacgaatt tcagcgattt
     9061 aaccacttcc cgggtgatat cgcgcttttc cgttagccgc gacaacgtgg gcagcaaata
     9121 tacgctgaac gatgccgtaa taaattgcag gtaggcatcg gaaatactgc tcaccccttg
     9181 ccagatcccc acctcatccc agctatactg cgccgccagc agtttacgca tcatgatgta
     9241 agcaacaggc aaggtcaccg acgtaatcaa cgtcatgagc gtaaatttgc tcaactgccc
     9301 tgccagaccg ttatcccagc tgggtttcag atagcttaac gggatagcac cacgcttaat
     9361 taacatgacg gcggcaggaa tcaccaccag cgcgggaatc aacgccagac ccagcaacgc
     9421 cccttcatat ccgcccaaac ggtaactgac gtagtacgca agaacgccaa tcaagctgcc
     9481 aacaatcaga gataacgcat tacctgcggc atcgcgaaag cctttcatca acgccagtaa
     9541 caggttgccc caggcgatcc ccatttgcac cagcgccact aaacgcacca gcccctgata
     9601 gtcgtgatta ccaaacaatc ccaagctgat tggcgcagct gccagcacaa aaaccagcgc
     9661 catcagcgta gagaagccaa gtaccatcgc tgatgaagtg ccgaccacgc ggcgcagctg
     9721 ttgcggatta tcatggtact gggcaacgta tttggttaca ccgttaaaga tgccagcccc
     9781 ggcaagcacg ccgagcacgg taatcaactg gcggaaattt gccgccagcc caagccccgc
     9841 cggaccaaat gacaccgcca gcaacttacc gaccagtaac ccggcaccaa tcttgaccag
     9901 tgtactggcc gccgtccaca aggacgcttt tgccaacgac atatcaggag aagtagttca
     9961 acaaagtcgc aattaccgta cgctgattga cgggcgacag gttgtagaac agcggcaggc
    10021 gcagcaggcg ctcgctctct ttggtggtgt agcgatcttc accgtggaac tcaccaaagc
    10081 gttcccccgc agggcaaccg tgcagcggaa tgtaatggaa caccgccatg atttccgctt
    10141 ctttcagaaa gttaatcaac gcgctccggt caccaatatc ccgcagttta atgtaaaaca
    10201 tatgcgcgtt ctgcaagcag ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg
    10261 ccagaggtgc taacgcatcg tagtagtttt gccacagcgc cagacgttgc tggttgatac
    10321 gatccgctgc ttccagttgc gcccacaggt acgcagcttg cagatcggac atcaaatagc
    10381 tggagccgat atcgcgccag gtatatttat cgacctgacc acggaagaac tggctgcggt
    10441 ttgtgccttt ttcacggatg atctcggctc gttcgatcaa cgctttatcg ttaatcagcg
    10501 tcgcgccgcc ttcaccgccc gccgtgtagt ttttggtttc atggaagcta aagcagccaa
    10561 tatgaccaat ggttcccagt gcacgccctt tgtaagtgga catcacgccc tgagcagcat
    10621 cttccaccac aaacagatta tgctttttcg ccaacgccat aatggtgtcc atttcgcagg
    10681 ccacgcccgc gtaatgcacc ggcacgataa cgcgcgtttt gtcggtgatc gccgcttcaa
    10741 tcaacgtttc gtcgatgttc atggtgtccg ggcgaacatc cacaaaaacg atttttgcgc
    10801 cacgcagcac aaaggcattg gcggtggaga caaaggtgta gctcggcatg atcacttcat
    10861 cgccaggctg gatatcgagc agcagcgccg ccatctccag cgaagcggtg caggacggcg
    10921 tcagtaacac tttggcgctg ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa
    10981 aaccgccatc gccacacagt ttgccgctac ccattgccga ctgcatatag tcgagttcgg
    11041 ttcccaccac cggcggtgcg ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg
    11101 gtgctttcta cattcgcacc actttgtatg tatcgtttaa gcgcggcggt gttgcccatt
    11161 tgggtcgcca cccgcaaagt tgttttaccg cgagcatacg cccagtttag cgccgtttgc
    11221 atcagctcag cacctgcacc gcgtccagcc agcaggccaa ttcgcgcatc tgtagcattg
    11281 agttcccgta aagagacata accgcgaata tcgccggacg ccgcacgtaa aatcagacat
    11341 tgatgatcaa aggtgccgcg cacggcattt tcaatccact gtgcataaaa gcgactgctg
    11401 gcgtcaggcg cataccacgg cgcacgaaaa cggctttgcg caaatgcggc gctggctaac
    11461 tgacgtaatg cgggaatatc ggtctcttgt gccactacag caccgctatc actggcattg
    11521 ttcacgggta gcgccaaatc aacttcacct tctaccaggg agaatccctg ctgttgcagg
    11581 gcatccagtt cccccgtatt tgatgccgca attttggcct gcacccgtga ccacggcgct
    11641 aacgcgtctg gcgtcaggaa cggtgcttca gacgtaatgc gcacgatggc gctgttaaca
    11701 ccaaagaagg cgttttccca ggttagtggc tcaatactgg cgcggacggg cacgaagtaa
    11761 ctccagcaga tattggccgt agccagtttt cgctaatgaa ctggcagcac gcttcacacc
    11821 ctcgtcatcg agccagccgt tacgccaggc gatctcttcc aggcaggcaa tcttaaagcc
    11881 ctggcgtttt tccaccgtct gtacaaaggt gctggcttca atcaggctgt cgtgagtgcc
    11941 agtgtccagc caggcaaatc cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag
    12001 gtacatctgg ttgatggagg taatctccag ttcaccacgc tccgacggct tcacctgctt
    12061 tgcgtactcc acgactttac tgtcgtagaa ataaagcccg gtcaccgccc agtttgattt
    12121 cggctgtttt ggcttttctt ccagcgagat agcgcggaaa ttatcgtcaa actccaccac
    12181 gccaaagcgt tccgggtcca tcacctgata gccaaaaacc gtcgccactt ccgtgcgcgc
    12241 cgcaacatga cgcagcttcg gactgaagcc ctgaccgaag aagatgttat cgcccagcac
    12301 cagacaagaa ggttcaccat taaggaaggt ttcaccgatg ataaaggcct gcgccagacc
    12361 gtccgggctg ggctgttcgg catattccag ctggataccg aactcactac catcgcccag
    12421 caggcgctgg aaataacctt tatcttccgg cgtagtgatg atgagaattt cgcggatacc
    12481 ggccagcatc agcaccgaca gcggatagta aatcattggc ttatcgtaaa tcggcaacag
    12541 ttgcttcgat acgccgcgcg taatcggatg caatcgggta ccggaaccgc ccgccaggat
    12601 aatacctttc atgtttgcct ccgctgaaaa ttagcctttc agacctaaac gctcgccctg
    12661 atagctgccg tcctgcacct gcttcctcca gctttcatta gccagatacc actgcaccgt
    12721 tttacgcatt ccactttcaa aggtttcctg cggcagccag ccaagttcac gggcaatttt
    12781 cgaagcatca atggcatagc gcagatcatg ccccggacgg tcagcgacaa aggtgatcaa
    12841 gtcacgataa tgcaccacgc cttgcggctt gttcggagcc agttcttcca gcagctcgca
    12901 aatggtttcc acaacatcga gattcttacg ctcgttgtga ccaccaatat tataggtttc
    12961 accgactttc ccggtggtcg ccacgcaata cagcgcgcgg gcgtgatctt caacatacag
    13021 ccagtcacgg atttgctgcc cgttgccata taccggtagc ggtttacccg ccagcgcgtt
    13081 gaggatcatc agcgggatca gtttttccgg aaagtggtaa ggaccgtagt tattcgagca
    13141 gttggtgata agcgttggca gaccataggt tcgcagccag gcgcgcacca gatggtcgct
    13201 gctggctttt gacgcggaat aagggctgct cggcgcatac ggcgtggttt cggtgaagaa
    13261 atcatccgtc gagtgcaggt caccatatac ttcgtcagtg gagatatgat gaaaacggaa
    13321 cgctgatttt ttatcttccg tcagcgcatt ccagtaagcc cgcgccgctt caagcaatgt
    13381 ataagtcccg acaatgttgg tttcaataaa cgctgccggg ccgtcaatag aacggtcaac
    13441 atggctttcg gctgccagat gcatgacaca gtctggctga tgctcagtga atacgcgcgc
    13501 cagttctgcc cgatcgcaga tatcaacttt ctcaaaggca aagcgctcgc tttgcgcgac
    13561 cggtgccagc gacatcaggt ttccggcgta ggtcagctta tcgaccacta ccaccgcgtc
    13621 gctcgtttcg ttgatgatat aacgcaccag cgccgagcca ataaacccgg caccgcctgt
    13681 tatcagaatc tttctcatca gcgccagact cctttagcat cgacgacata ctgctgatgg
    13741 acattgtcgc cattgataac tttgaactga ctatgatcga ccagcatcac cagcacgtct
    13801 gccgttgcca gcgcctcgtc aagctgcgcc agagtacaaa gcccggtcag ttttttcggc
    13861 aactggtgga tgttaggctc aacaaccaga gtttcgccgc tatgccactg ggcgatcagt
    13921 tcagcgattt ccatcgccgg gctttcgcgc aggtcatcaa tattcggttt aaacgccaga
    13981 ccaaagcagg cgattttcag ttcactggcg cgtttatcgg tagccgccag gcaatcagcc
    14041 accgccgctt tcacctgatc gataacccag aacggtttgt gatcgttcac ttcgcgcgcg
    14101 gtacggataa gccgcgcctg ctgggggttc tgtgccacga taaaccacgg atcaacagca
    14161 atgcagtgac cgcccacgcc agggccaggc tgaagaatat taacgcgagg gtgacgattc
    14221 gccaggcgaa tcagttccca gacgttaatc ccctgatcgg cacaaatcag cgacaattca
    14281 ttagcaaaag cgatattcac atcgcggaag ctgttttcgg tgagcttaca catttccgcc
    14341 gtccgcgagt tagtgacgac acactcacct tcgaggaaaa ttttgtacag ttcgctggcg
    14401 cgggccgaac aaaccggcgt cataccacca atcacgcgat cgtttttaat cagctcgacc
    14461 ataacctggc ccggtaacac gcgttccggg cagtaagcaa tgttgacgtc cgcctgctcg
    14521 cccacctgct gtgggaaagt gagatccgga cgcatctctg ctaaccattc tgccatcttc
    14581 tcggttgacc ccaccggcga ggtggattca aggatcacca gcgcgccttt tttcagcact
    14641 ggcgcaatgg agcgagcagc cgattcaacg taggtcatat ctggctcatg atcgccttta
    14701 aacggcgtgg gtacagcaat cagccaggca tccgcttcaa ctggcgtcgt gctcgctcgt
    14761 aaaaaaccgc cttctacggc agtttttact acgctcgcca aatcaggctc gacgatatgg
    14821 atttcgccac gattgatggt atcaaccgcg tgttggttga tatcgacacc aatcacctgt
    14881 ttttgccgtg aggcaaacgc tgctgctgta ggcagcccga tgtaacccag tccgataaca
    14941 gaaatggtcg caaaactcat agtgatatcc gattattttt taacgcttcc agaatgcgag
    15001 agcatgcctg accatcacca tacgggttat gggcgcggct catagtttga tattcgtttt
    15061 cgtcttttaa aagacgcgtc acttcctcga caattcgctg cttatccgtg cctaccagac
    15121 gcaccgtacc cgccgtcacc gcttccggac gctcagtggt atcgcgcatc accagcaccg
    15181 gtttccccag cgaaggcgct tcttcctgaa tgccgcctga gtcggtcaaa atcagccagg
    15241 cgtggttcat cagccagaca aacggtaaat actcctgagg atcgatcaga atgacatttt
    15301 tcacatgccc cagaatgcga ttgaccggct ctctgacgtt cgggttgaga tgcaccggat
    15361 agacaatctg gatgtcctgg tgcgtggtgg cgatgtctgc cagcgcgtgg cagatttctt
    15421 caaagccacg accgaagctt tcacgcctgt gaccggtcac cagaatcatc tttttatcgg
    15481 ggtcgataaa cgggtaattt gccgcaagtt ctgaacgcag cgtgtcgctg ctcatcacct
    15541 ggtcacgcac ccataacagt gcatcaatga ctgtattacc ggtaatgaag attcggctat
    15601 ctgcaacgtt ttcacgcagc aagttttgcc gggaagtttc ggttggagag aaatgataca
    15661 tcgccagatg cccggtcaat gtacggttag cctcttccgg ccacggcgaa tagagatcgc
    15721 ccgtgcgtaa accagcctca acgtgaccaa caggaatacg ctgataaaac gccgccaggc
    15781 tggttgccag cgtcgtcgtt gtgtcgccgt gaaccagcac gacgtctggt ttgaactcgg
    15841 caagaatagg ttttagccct tccagaatcc gacaggttat ctctgtcagg ccctgtcctg
    15901 gctgcattat gttgagatcg tagtcaggta caatggaaaa gagtttcagc acctgatcga
    15961 gcatctcccg atgctgcgca gtgacgcaaa ctttagcctc aaaaaaagga tcttttgcca
    16021 acgcatgcac caacggagcc atcttgatgg cttccgggcg cgtaccaaat acagtcagta
    16081 ctttcacatc gattctcttc gaataagcgg cgagcgcctt tgcgctcacc gcagcagtgt
    16141 tgctatttcg agcaacggcg ggttaatgcg acaccagccc cgatcagccc cccgacaatg
    16201 ccccacataa tcatcaggaa ggcacgacgt gggctatcgc gttttaccgg ttcttccggc
    16261 gtacgcaaat agcgataggt ctgaaaacgc ggatccaggg ttggaccaac attcagggtg
    16321 tttaacatgg cccgattctg atcatagtcg agatcaaagg ccggaccgac ggcctgtaaa
    16381 ttttccagtc gagcctggag cattggacgc ccaagcagga acatttctga atcaggtaat
    16441 tcctcggcag gcacatctgt cgcactgcgc gaaatattat gctgctcagc aattttcagc
    16501 gcctgctcaa tgctgttcat ccggcggtcg tagatggctt tcgccacctc ttcctgacgc
    16561 ttcacctgtg ctttcatctg gatggtacgc gccgcccatg cgcctttcag ctcatcattc
    16621 agatggctgg ctgcacgctg gctggcaaaa gcaacatact gacgtaacag gttattagcg
    16681 tcaggcgcgg tttcggcaat aagcttcacg ctgtcattga ccgcgcgggt aaagtctccg
    16741 gggataaact ggatgttgtt aatcatttca tccagcaacg ccgcatcggc tttgctgttg
    16801 cccaccatcc gctgcttgta atagtcggtt tgcagccaga actctctgcg ggtatcccac
    16861 gaggccagtt gcataacaaa ctctttatag gcttcgtcca tgaccgatgg ttggtcggca
    16921 gaagccatgt ttgaacggac atccaggtta cgcaaaaatt gctgctgcga gtaatatccc
    16981 cccagcatat tcaccgttgg acgatcggta atcgccgtcg agctccactc ctgacgagca
    17041 aaaaacgtat acgccagcgc gattaacgca aacgccagcc ccatgccaat aatccatagc
    17101 ttcccagccc acaaggtacg aaacaaccca cgaatatcca gttcattttc agcgtcttcg
    17161 gccggtttcc caggcattgg ttgtgtcatc acatcctcat ttatttggtt aaattggggc
    17221 tgccaccacg atttctacgc agtctgcgtt ttacgcgctt aataaagcga gcaactttcc
    17281 aggcacgctt aatgcaatat ccatagagga ggaatgctag caaaaagagc accaacatga
    17341 cccactccgg gacaaaatga gaatattctg ccagcacgcc aatggaagcg agcagtgctg
    17401 cggcaagggt aatcagcaca aacgcctggc gggaagtaaa cccggcacgc atgatcaaat
    17461 ggtgaatatg ctgacggtca ggagagaatg ggctcatgcc tttacgcagg cgacggtaca
    17521 taatcgccac catatccatt agcggaatgg cgattatcca caaagcggta accgggctga
    17581 tgggatgggt tttgccttgg gtcgtttcga gcaggatcca gatcacggta aaaccaatca
    17641 gcgtactgcc cgcatcaccc ataaagactt tgtagcggcg acccaggata ccaaggttaa
    17701 gcatgatata tggcaggatg gcggcgatca tcgcaaagca ccagattgcg aggctggttt
    17761 gcccgtcgaa ccacaaaatc ataccgattg ctgcaaacga gacgcaggac aacccgccca
    17821 gcaagccatc aatgccatca accatgttga acgcattaat ggccgcccag acggcaaata
    17881 gcgtcaggaa gtaaccaaac ggtccgagca ccatctccca ggagccaaag atataaccca
    17941 ggctactgag ataaaggttg ccaaacacca tcataacaat gccaacagcg gcctgtatgg
    18001 tggcacggat ttttacgctg atatcaaaac ggtcatccag cgcgccaatg aaaacaagca
    18061 caccggcaca agcgagatag agagatgcat gcggaatata gtaatcgaca attccgaacg
    18121 tgaagcaaat ccctgcgtaa accgaaatac ccccaacgag tggtatcaac ccctggtgac
    18181 gtttgcggaa gtttggttta tccactaaac cgactttttt tgccacctta cgggcaaaaa
    18241 acagaaacag tgtcgtgaat aaaaaaatac tgatgagatc agtactcact gtcagtaaat
    18301 tcacaatgca tgctctcaga gaaaattatt agcagaagta taaccacgaa gacctttatt
    18361 cagaagggaa agtccgttcc gaacccacca gtcctgtgca aaaattaacc atttgatatg
    18421 caaattgcat aacatgccag caaattccag gaattttcct aacatcgcca gcgcggcatt
    18481 aagattacag atataaaagc aaaacgccac gtaaacacgt ggcgtttttg gcataagaca
    18541 aatttatgag cgtttcatca tttcgaagaa atcgtcattg gtcttggtca ttgccagttt
    18601 attaatgagg aattccattg catcgatttc gcccatcggg tgaatgattt tgcgcaggat
    18661 ccacattttc tgcagttctt cctgagtcgt gagcagctct tctttacggg taccggaacg
    18721 gttgtagtcg atagccggga agacgcgttt ttcagcgatc ttacgagaga ggtgcagttc
    18781 catgttgcct gtacctttaa actcttcgta aataacttcg tccattttag aaccggtatc
    18841 gataagcgcc gtcgcgataa tggtcaggct gccgccctct tccacgttac gcgccgcacc
    18901 gaagaagcgt ttcggacgat gcagggcgtt ggcatccaca ccaccggtca acactttacc
    18961 tgacgccgga acaacggtgt tgtaagcgcg cgccagacga gtgatggagt cgagcagaat
    19021 gataacgtct ttcttgtgct caaccaggcg tttcgccttc tcgatcacca tttccgcaac
    19081 ctgaacgtgg cgagatgcgg gttcgtcaaa ggtagaagca acaacttcac cttttaccag
    19141 tcgctgcatc tcggttactt cttccggacg ttcgtcgatc agcagaacca tcagcacaca
    19201 atccgggtgg ttgtaagcaa tgctctgagc aatgttctgc agcagcatgg ttttaccggc
    19261 tttcggcggt gccacaatca gaccacgctg accacgaccg ataggtgatg ccagatccag
    19321 tacgcgagcg gttaaatctt cagtagaacc gttaccacgt tccatacgca gacgagagtt
    19381 tgcgtgcagc ggggttaagt tctcaaagag gattttgttg cgggcgtttt caggtttgtc
    19441 gaagttaact tcgttaactt tcagcagcgc aaaatagcgt tcaccttctt tcggcgggcg
    19501 aatcttacca gagatggtat caccagtgcg gaggttgaaa cggcggattt ggctagggga
    19561 aacgtagatg tcatcaggac cggcgaggta ggagctgtct gcggaacgga ggaaaccaaa
    19621 tccatcctgc aatatctcca gtacgccatc accaaagata tcttcgccac tctttgcgtg
    19681 ctgcttcagg atggcaaaaa taatgtcctg cttacgcata cgagccaggt tttccagccc
    19741 catattttcg ccgagagtga tcagctcaga aaccggcgta ttctttaatt cggtaagatt
    19801 catagtggtg tgagttctta aacttggggt aaatctcgaa cttaatgttg tgaatggtat
    19861 ggcaggatca tccatgcctg tttacggtca tcgactcatg tcttttcgct gcctggtcac
    19921 aggagagtac gcagaactga aacgacaaga cggattgagt gacgagccag aaatctgttc
    19981 acttcgcatt taagataaaa cgggaagcgt tggaaattac aagattcaaa cttaataagg
    20041 tatgtttaat acgaagtcaa cactaagtta gcatgactca cgccgggcgt ccagttttta
    20101 gcgacggggc acccgaacat gaaattccct tacgccaggt tagcgtcgag gaactctttc
    20161 aactgacctt tagacagtgc gcccactttg gttgccgcca cttcaccgtt tttgaacagc
    20221 agcagagtcg ggataccacg gatgccatat ttcggcgcag tgccagggtt ttgatcgatg
    20281 ttcagttttg caacggtcag tttgccctga tattcgtcag cgatttcatc cagaatcggg
    20341 gcgatcattt tgcacggacc gcaccactct gcccagaaat cgacgaggat cgccccgtcc
    20401 gctttgagta catccgtgtc aaaactgtcg tcagtcaggt gaataatttt atcgctcata
    20461 tataactcca caggaataag cctggcgtgt tggtttcgtt gttggtgtaa cattaaccaa
    20521 ctaaaggttg actttatttc accggatacg ctttcgtaaa gcaatagtaa gctgatattc
    20581 taccacacta tgagcaaaac acatttaaca gaacagaagt tttccgactt cgccctgcat
    20641 ccgaaggttg tagaagccct tgaaaaaaaa gggtttcata actgtacgcc cattcaggca
    20701 ctggcccttc cgctgacgct ggcgggtcgt gacgtagccg ggcaggcgca aaccggtacc
    20761 gggaaaacga tggcgtttct tacgtcaacg tttcattgtc ttctctctca tcccgcgatt
    20821 gccgatcgca aggtgaatca gccgcgtgcc ttaattatgg caccgacgcg tgaacttgcc
    20881 gtgcagatcc atgccgatgc ggaaccgctg gcggaagcta ccggcctgaa gctgggtctg
    20941 gcttacggtg gtgatggtta cgacaaacag ctgaaagtgc tggaaagcgg cgttgacatt
    21001 ctgattggca ccacggggcg tttaattgac tacgccaagc agaaccacat taacctcggt
    21061 gccattcagg tagtggtact ggacgaagcc gatcgcatgt acgatctggg ctttattaaa
    21121 gatatccgct ggctgttccg ccgtatgccg cctgcaaacc agcgcctcaa catgctgttc
    21181 tccgccacgc tttcgtaccg ggtacgtgaa ctggcgttcg agcagatgaa caatgccgaa
    21241 tatattgaag tggaaccgga acagaaaacg ggccaccgta ttaaagaaga gcttttctac
    21301 ccttctaacg aagaaaaaat gcgtttgctg caaacgctga tcgaagaaga gtggccagac
    21361 agagcgatta ttttcgccaa taccaaacac cgttgtgaag agatctgggg ccacctggcg
    21421 gcagatggtc atcgtgtcgg tttgttgaca ggcgatgtcg cgcagaaaaa acgtctgcgt
    21481 attcttgatg aatttacccg tggcgatctg gatattctgg ttgccaccga cgttgccgcg
    21541 cgtggtttgc atattccggc agtgacgcac gtctttaact acgatttacc cgatgactgt
    21601 gaagattacg ttcaccgtat tggtcgtaca ggtcgcgcag gcgcaagcgg tcactctatt
    21661 agcctggcgt gtgaagagta tgcattgaat ttgcctgcta ttgagaccta tattggtcac
    21721 tcaattccgg taagcaaata caatccggac gcattgatga ccgatctgcc aaaaccgctg
    21781 cgcctcacgc gcccgcgcac aggcaatggt ccgcgtcgta ctggcgctcc gcgtaatcgt
    21841 cgtcgttcag gttaaataaa aaatgtcata ctcaaaatca ttcaaactgt atcaagatgg
    21901 caagagaatg aatcccgatg agcttactgt agtaagtgat tcaggtgaaa gaatgcagcc
    21961 aacacagaga cagattgaag gatgaagagt atgggttcca cctcatcgct gtatgcagcc
    22021 attgatctcg gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc tggaagcatc
    22081 cagacgctga cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa tagcgaaaat
    22141 gccctctcca atgaagcaat ggagcgcggt tggcaatgtt tgcgcctgtt tgctgaacgt
    22201 ctgcaagata ttcctccctc gcaaattcgc gttgtcgcta cggcgacgtt acgccttgcc
    22261 gtcaatgcgg gtgattttat tgccaaagcg caggaaatcc tcggttgtcc ggtacaggtg
    22321 atcagcggtg aagaggaagc acgtctgatt tatcagggtg ttgctcacac cactggcggt
    22381 gccgatcagc gcctggtagt ggatatcggc ggtgccagta ctgaactggt aaccggcacg
    22441 ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg gctggaacgc
    22501 tattttgccg atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa agcggcacgc
    22561 gaagtgttac gtccggttgc cgatgaatta cggtatcacg gctggaaagt gtgcgttggt
    22621 gcttccggca ccgtgcaggc gttacaggaa atcatgatgg cacaggggat ggatgaacgc
    22681 attaccctgg aaaagttgca gcaattgaaa cagcgagcca ttcattgcgg tcggctggag
    22741 gaactggaga ttgacgggct gacgctggaa cgtgcgttag tgttcccgag tggtctggcg
    22801 atcctgatcg ccatttttac cgaactgaat attcagtgta tgaccctggc gggcggtgcg
    22861 ctgcgtgaag gcctggtcta cggcatgttg catctggccg tcgagcagga tattcgcagc
    22921 cgtacgctgc gtaatattca acgccgcttt atgatcgata ttgatcaggc acagcgcgta
    22981 gccaaagttg cggctaactt cttcgatcag gtggaaaacg aatggcatct tgaagcaata
    23041 agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag cgttgacttc
    23101 aaacaagcgc cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc cggttttacc
    23161 cccgcacaga aaaaattgct ggcgacgcta ctgctcaacc agactaatcc ggtcgatctc
    23221 tcatcgctgc atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact ctgccgttta
    23281 ctacgcctgg ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc agagatgaca
    23341 ttacaggcta accatgaact gttgaccttg acgcttccgc aaggttggct aacccaacat
    23401 ccgctgggta aagagattat tgctcaggaa agccagtggc agagctatgt ccactggccg
    23461 ctggaagtgc attaatgagt aagtgccgga tgcgatgctg acgcatcttt tccggccttg
    23521 attatttccc tcgttttgcc gccatcatcg ctttcagatt cgccagatgg ctttgccctt
    23581 tctgcatccg ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc agatcatcct
    23641 gcggcagctc cagcaaaaag cggctcggct ccgggcgcac cagttcgccg tactgacggc
    23701 gttctttaca cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg acataggcca
    23761 gccgccgctc ctcatcgata ttatcttcat cgatactgct ctggtgcggc aaaaaccctt
    23821 cttccatacc gaccatgtag acataaggaa actccagtcc ttttgacgcg tggagagtca
    23881 tcagttgcac ctgatccagc tcttcttcac tctcaccacg ctccatcatg tcgcgcaaag
    23941 taaagcgcgt caccacctgg gtgagcgtca tcggctcatc cagttcgctg ccttccagca
    24001 tctccgtcat ccagctaaac agttggttga cgttcttcat gcgcatttcg gcggctttcg
    24061 ggctgggcga tgtttcgtac agccaggatt cataatccat gccatggatc agatcacgca
    24121 ccgcagcaat cggctcccgc tccgccaggc gctggatttc tgccaaccag tgagtgaagc
    24181 gggtcaatgc ttcataacca cgtccgctaa gcgtctgact caggcccata tcaaagctgg
    24241 cggtaaacat gcttttattg cgcgtcatcg cccactcacc cagctttttc agcgtagccg
    24301 ggccaatctc tcgcttcggc gtgttaacga tacgcagaaa tgcgctgtca tcgtccgggt
    24361 tagtcagcac gcgcagataa gccagcaagt ccttgatttc aggacgagag aaaaacgacg
    24421 taccaccgga tattttgtac gggatgcggt tttgcatcag gaatttttca aatacccgcg
    24481 actgatggtt gccgcgataa agaatggcgt aatctttgta ctgcgtttta ttgacgaagt
    24541 gatgggcgat cagctcgcca gtaacgcgct cggcttcatg ttcctcgtta ttcgcgctta
    24601 aaacttttag ctctgtgccg taacccagtt cggagaacag acgcttttca aagacgtgcg
    24661 ggttattggc gatcaggatg ttcgccgctt tcagaatacg cccggaagag cgatagttct
    24721 gctcaagctt aatcaccttc agcgccggaa aatcctgact cagcagcacc aggttttgcg
    24781 gacgtgcacc gcgccaggag tagatcgact ggtcatcgtc acccaccacg gtaaagcgcg
    24841 cgcggctgcc caccagcagt ttcaccagtt catactggct ggtgttggtg tcctgatact
    24901 catccaccag caggtagcga attttgttct gccagcgttc gcgtacttct tcattgcgtt
    24961 gcagcagcaa cgtcggcaat aaaatcaggt catcgaagtc gagaacgtta caggctttca
    25021 ggtgtgcatc atacagccca taacaatggg caaaaatacg atcccgctcg ccctttgcct
    25081 ctgccgccgc ctgcgccgga gttttgagat cgttcttcca gttagagatg gtcgaaatca
    25141 gttgttgcag gagaactttg tcatcttcaa tcagcccctc ggtcaactct ttaagcaaag
    25201 caagctgatc ggtatcgtca aataacgaga agttcgcttt catcccaagc gccgcatact
    25261 cgcgtttgat gatatccagc cccaacgtat ggaaagtgga gatcatcagc ccacgcgcct
    25321 ctttacgccc cagcgtctgc cctacacgct ctttcatctc gcgcgctgct ttattagtaa
    25381 aggtcaccgc cgcaatgtgc cgcgcctgat aaccgcaacc gcggatcaga tgggcgattt
    25441 tattggtgat aacacgagtt ttaccggaac ccgcgcccgc cagcaccagg caggggccgg
    25501 taacgaattc gacagcttgt tgttggccgg ggtttagacg cataggtgta ttgctcaatc
    25561 ttcgaacggg gggaggattg tagcagaatc gcatttgaat gtgaccgcgt gtaagccgct
    25621 aaccggttgg ctgcggatgt ttaaatcaga gagagaagac cgaaatcgga gttcatcgtg
    25681 aatccattag acttagaaaa tatcgggtcg taacgttgtg ttattacttt ttcagagttt
    25741 actaaggaga ggtttactcg tggcgattaa tttcagccct aaggttggtg aaatactgga
    25801 atgcaacttt ggtaactatc ctgttagcca aaatggaccg ttcagcacga cgtattacga
    25861 tggccgcatt cctcctgaaa tgataaaaaa caggttagtt gtcgtgctca acggaaaaat
    25921 taatggcaac gcattcatcg tcgttccatt atcaaccact cgcgaccatg acaagttaaa
    25981 acgtggaatg catgttgaaa tagccagtaa tgttatcaat gacctgcaat tctttgatca
    26041 gcagattcgt tgggccaaaa ccgacctggt acaacaagtc agtcgaaaca gattaaacag
    26101 agcaagaact tacagagggt atcttaatca atgtttgcca catgagttgg ttgcagacat
    26161 acagagggcg gtcatcaagt caatcaatgc catatcgctc ataaattgac aataaaatca
    26221 ccatccccga taatgacttt gtagcccata gaggccctaa gttttctccc cttgagggac
    26281 ctgcgaagcc cggattttcc gggcttttgc catatatgta ccagcccctc cctgactgac
    26341 aattacgtta gcatcggcgg atcatgctaa aatcgccgcc tgacaattat tcactcaagg
    26401 cacgatcatg gcaaaaacag cagcagcact gcatatcctt gtaaaagaag agaaactggc
    26461 tctggatctt ctcgagcaga ttaagaacgg ggccgatttt ggcaagctgg cgaagaaaca
    26521 ctccatttgc ccatcaggca aacgcggcgg tgatttaggt gaattccgcc agggtcagat
    26581 ggttccggcg ttcgataaag ttgtgttctc ttgtccggta ctggagccaa ccggcccgct
    26641 gcacacccag ttcggttacc acatcattaa ggtgctgtac cgtaactaat agcaaggcct
    26701 tctccgggag aaggccttga actctgaatc gctattaacc cgcaacagca atacgtttca
    26761 tatcagtcat gtagccgcgc agtttcttac ctacctgctc aatcgcatgg ctgcgaatcg
    26821 cttcgttcaa atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
    26881 aatcgcccgg ttgcagctct gccataaacg gtttcagcaa cggcacacaa gcgtaagaga
    26941 acagatagtt accgtactca gcggtatcag agataaccac gttcatttcg tacagacgct
    27001 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
    27061 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
    27121 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
    27181 gcggcgcggt ttcaaacgcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
    27241 catcgttggc ccagtccgcc atcatgccgg aggagaattc gccggagatg atgtcgtcca
    27301 tatgtttctg gaacagcggt gccatgatct ctttcagctg ttctgacaac gcgtaagcgc
    27361 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgtttcagcg
    27421 cttcggtgat ggtttcccag ccgaactgaa tcagtttttc tgcgtatgcc ggatcggtgc
    27481 cttcttccac cagcttgtcg aagcacagca gagagccggc ctgcaacata ccgcacagaa
    27541 tggtttgctc gcccatcagg tcagatttca cttccgcaac gaaagaggat tccagcacgc
    27601 ccgcacgatg accaccggtt gccgccgccc atgctttagc gatcgccatg ccttcgcctt
    27661 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
    27721 cttcacgtac ttcggtgcct gggcatttcg gcgcaaccat tactacggtg atgtctttac
    27781 ggatctgctc gcccacttcg acgatgttga aaccatgcga gtaacccagc gccgcgccgt
    27841 ctttcatcag tggctgtacg gtgtgcacta catcagagtg ctgcttgtcc ggcgtcaggt
    27901 taaccaccag atccgcctgt gggatcagtt cttcgtaagt acccacttta aaaccatttt
    27961 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
    28021 agatatcgag accagaatca cgcatgttca ggccctggtt cagaccctgt gcgccacagc
    28081 cgacgatgac cactttttta ccctgaaggt agctcgcgcc atcggcgaat tcgtcgcggc
    28141 ccataaagcg acatttgccc aactgtgcca gttgctggcg caggttcagt gtattgaagt
    28201 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcaa
    28261 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacatcat
    28321 attgcaattt ttgcaacgtc aacatcgagg gctgtccctg tggatttacg cgatctgaaa
    28381 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
    28441 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatctcgg tcagccgctg
    28501 tttgtgcgcg ataaccgcac ggtgacgctg actgaagcgg gcgaagaact gcgcgttttc
    28561 gcccagcaaa cgctgttgca gtatcagcag ctgcgccaca ccatcgatca gcaagggccg
    28621 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
    28681 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agattaaact cactactggt
    28741 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
    28801 aaaccggaga cgctacccgg cgctgtagcg ttttcgatgc tggagaatct ggcggtagtg
    28861 ctgattgcgc ccgcgctgcc ctgcccggtg cgtaatcagg tctcggtaga gaagccggac
    28921 tggtcaacgg taccgtttat catggccgat caggggccag tacgccgccg cattgaactg
    28981 tggtttcgac gcaataaaat cagcaaccca atgatttacg ccacggttgg cgggcatgaa
    29041 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
    29101 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
    29161 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
    29221 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgagtag gcctgataag
    29281 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccgggttatt
    29341 ggtttcgtcg tggcaatcgt agcccagctc attcagccgg gtttcgaaat ccggttcatg
    29401 gtcgccaagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
    29461 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
    29521 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
    29581 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
    29641 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
    29701 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
    29761 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
    29821 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
    29881 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt
    29941 gtgctgggcg atatattttt tcattcccgc cagcgccagc gcgccagagg gttccgccac
    30001 cgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
    30061 gatgatatcg tcgagatact cctggcacaa acggaaggtt tcgtcgccga tgcgttttac
    30121 cgcaacgcct tcagcaaaca gccccacgcg cggcagatca accggatgac ccgcatccag
    30181 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg attactttga tttgcggcat
    30241 cagttgtttg atcagcaccg ccacgccagc agccagaccg ccgccgccga ctggcacaaa
    30301 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
    30361 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
    30421 gatcgctttg gctttcgctt catcaaagtt cgcgccgtgg agcagcactt cgccgccgaa
    30481 gccgcgcacc gcgtcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
    30541 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
    30601 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
    30661 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatca cgttatcaag
    30721 acgagacgag agcttttcca ttttttgtag cggcgtgacc tgcgccgctt cgtagaccgg
    30781 cgcgcgcagc accgctctta aatattcagc accttccggg gcaccggaca ggggttgcga
    30841 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcgccttt gtcggcgctg
    30901 gttgccaggc tggcataagc acgcagggca aaggagatct gacgttcacg atttttcggc
    30961 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
    31021 cttacctgta actgaatgcc acggttcgga atgtcgatgg cgatcaggtc gccgtcttca
    31081 atcaggccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat agaaaggcca
    31141 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagccccatt
    31201 gatttcagga agctggttgg gtagagcatt tcctgcatcc ccggaccacc ttttgggcct
    31261 tcatagcgaa ttactaccac atctccggcg acaactttac cgccgagaat cgcttctacc
    31321 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
    31381 acgcccgcag ttttaacgat gcagccgttt tccgcgaaat taccgtagag caccgccagg
    31441 ccgccgtctt tgctataggc gtgttccagc gagcggatac agccattggc gcgatcgtcg
    31501 tccagcgtat cccaacggca atcttgcgag aatgcctggg tggtacggat acccgcaggg
    31561 ccggcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
    31621 agcgtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgca
    31681 cgatccagct cgccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
    31741 tatttttggg tgctcggcgc gactttgcat aactgcggaa ccttgcggga aagcttatcg
    31801 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacg
    31861 gtgttagtcg atccacccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
    31921 ctagcaatat tgcggggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
    31981 tcaacaatgc gtttaccagc attgaggaac agctgcttac ggtcagagtg ggttgccagc
    32041 agcgagccgt tgcccggctg cgacagaccc agcgcttcgg tcaggcagtt cattgagtta
    32101 gcggtaaaca tcccggagca ggaaccgcag gttgggcatg cggaacgttc aacctgatcg
    32161 ctctgggagt cagatacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
    32221 ttgatgatct gatcggacag tttggttttc ccggcctcca tcgggccgcc ggaaacaaag
    32281 atcaccggaa tattcagacg cagggaagcc atcagcatcc ccggggtgat tttgtcacag
    32341 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
    32401 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat ggcaatccca
    32461 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
    32521 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
    32581 ttcacaaccg cgataatcgg cttaccgaaa tcggcgtcgg tcattccggt ggcgcgccac
    32641 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
    32701 atactttatt tactcccagt gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt
    32761 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccggtaaa
    32821 gaggccgaag aaggcttgct gaatgcgttt ggtgaccggg ccacaacggc cttcgccaac
    32881 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaagac
    32941 ttcatccgcc aggtacaagg attcgcgcga cagcacctgc tcacgcactt caattcccag
    33001 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa
    33061 cggtggggtg aacagcacac catctttcac ttcaaacagg ttttcgcctg cgccttcaga
    33121 gatataaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
    33181 acccaccagc agggaagaga gatagttacc accggctttt gccgcagttg ggatggtgtt
    33241 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
    33301 gcccagatac gctccccacg ggaaagcggc gataatcaca tcggttgagt atcccgctgg
    33361 cgggtttacg cccatgccaa catcaccaac gaagatcagc ggacggatat aggcgctggt
    33421 gagattgttt ttgcggatca cgtcacggca agcttccatc agctcatcaa tgctctgcga
    33481 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
    33541 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc
    33601 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
    33661 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
    33721 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
    33781 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
    33841 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
    33901 gatgacgcac cacgcgtaaa acacgttcta aggtttccgg attgaagcga gccgatacat
    33961 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg caccaggcgg
    34021 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
    34081 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
    34141 agggatgccg aaggcgctgg ctaacttgag gaaatcgggg ttatcggtaa gggtggtttc
    34201 gctgtatcgt tcctggaaaa acagttgctg ccattgtcga accatcccta accgttggtt
    34261 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgtac
    34321 attcatcatg aaagagccgt caccggagat acagacgacg gtatcgtttg gtcgcgcgac
    34381 ttgcgcgcca accgccgccg gtaaaccaaa acccatggtg cctaagccgc tggaggtaat
    34441 gaaattttcc gggcgagtgt gtgcgatgtg ctgcgcggcc cacatctgat gctgccccac
    34501 atctgtggtc acaacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
    34561 cgcgtagata gcgtcaccgg gatggtcgta acgccaggca tgttcatcac gcagctgcgc
    34621 gcagtattgc tgccagtcat tgatatttaa tggctgctgt aatgctggta acagcgcatt
    34681 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
    34741 atccatatgg ataacactgg cgtgtggtgc gaaggtgttc aatttgccgg tcacccggtc
    34801 atcaaaacgt gcacccacgg cgatcagcaa gtcgcactcc tgcaccgcga agttcgccgc
    34861 tttggtgcca tgcattccca gcatgcccag atagtacgga taatctgctt caactgcgcc
    34921 cagccctttc agcgtgcagg tggcaggcat ttttgtggta gcgagaaatt ctcgtaaagc
    34981 aggaactgcc tgcgccatac ccacgccacc accaacgtac agcatcggtt tttgcgcttt
    35041 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
    35101 ggtggtgaac cacggttcca ggtcgccgct ggctaactgg atatcttttg ggatatcgac
    35161 cagaaccgga ccaggacgac ctgagctggc aacgtcgaat gcttcagcca taatgcgcgg
    35221 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcccag
    35281 gacatccact tcctgaaatg cgtcagtacc gataaacggt gcggacactt gaccggtgat
    35341 ggcaacaaca gggatagaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
    35401 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
    35461 agccgccatt gccgcaccct gctcatgtcg gcacagcaag tgctccacgc cgccgtcata
    35521 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
    35581 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
    35641 aatcttggga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
    35701 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
    35761 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
    35821 taatcacgac caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    35881 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atattttttg
    35941 gccgtttttt atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa
    36001 tagtgagtga aaaaaattca atttcagtac gaatgttgtt ccctccctcg tagttcatcc
    36061 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    36121 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    36181 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    36241 cagccctggg agtaaatgcg cccccaatca ctgttgaggt acatatcagt aaaggtttgc
    36301 ccggcttaac gatggtgggc ttaccagaaa caacggtaaa agaagctcgc gatcgcgtgc
    36361 gcagcgccat tatcaatagc ggatatgaat atccggcgaa aaaaatcacc attaaccttg
    36421 ctccagccga tctaccaaaa gaagggggac gatatgattt acctatcgcc attgcgttgc
    36481 tggcggcctc agaacagctt acagccaata agttagatga atatgaatta gtcggagaac
    36541 tggcgcttac aggcgctctg cgtggcgttc ccggtgcaat ctccagtgca actgaagcta
    36601 ttaagtcggg cagaaaaatt atcgtcgcga aagataacga agatgaagtg gggctaatta
    36661 acggtgaagg atgcctggta gccgatcatc tgcaagctgt ctgtgcgttt ctggaaggta
    36721 agcacgctct cgaacgcccg aaaccaactg atgcagtatc ccgggcgcta caacatgatc
    36781 tcagtgatgt tgtcggtcag gagcaaggaa agcgaggact ggaaattacc gccgctggcg
    36841 ggcacaacct tttactgatt gggccgccgg gaacaggtaa aacaatgctc gccagccgta
    36901 ttaatggtct tttgccagat ttaagcaatg aagaggcact ggagagcgct gcgatattaa
    36961 gtctggtaaa tgctgaatca gtacaaaaac aatggcggca gcgcccgttc cgctcacctc
    37021 atcacagtgc gtcgttaacg gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa
    37081 tttcgttggc gcataacggc gtgctttttc ttgatgagct acctgaattt gaacggcgta
    37141 cactggatgc tttgcgagag ccgattgaat ccgggcagat ccatctttca cgcacacgag
    37201 caaaaataac ctatccagcc cgtttccagc ttgttgcggc gatgaatccc agccctaccg
    37261 gacattatca gggaaaccat aaccgctgca cgccagaaca gacattacgt tatctcaacc
    37321 ggctctcggg gccctttctc gaccgcttcg atctctcact ggagatccca ttaccacccc
    37381 ccggcatttt gagtaaaacg gtagtgccgg gagaaagcag caccaccgtt aaacaacgtg
    37441 taatggctgc cagagagcgc caatttaagc ggcagaataa gttgaacgcc tggctggata
    37501 gtccggaaat acgcaaattc tgcaagcttg agagcgaaga tgcgcagtgg ctggaagaaa
    37561 cgctgatcca tctggggtta tcgattcgtg cctggcagcg gttattgaaa gttgcacgaa
    37621 ccattgctga tattgatcag tctgacatta tcacacgtca gcatttgcag gaggcagtta
    37681 gctatcgtgc gattgaccgt ttgctcatcc atctgcaaaa actactgaca taaaaaaagg
    37741 gcatttcgcc ctttttatta atcgtcagaa tcggtgtagt cttcagcacc ttcaacctgc
    37801 ggtttaccgc cggaaagggt gtgaaaacgt tttggacgct tgatacgcgt catatacttg
    37861 gaccacacgc gttctgcttc tgtcactggc tcacgttcgc cacggcatac tgctacgaag
    37921 agtttctctt cctcggtaac cggctcgcgt ttgccaagat ccaactcatt gaaggcataa
    37981 ccatgacgct caagcagttg tgcctctttg atggtgaaat caccatggcg agagaatcca
    38041 cgtggataat gtttattgtc gaaatatcga ttagtcgtcg taaagctttc cgccatcctg
    38101 cacgctccta attctttgac cgagctagtt atggcgcgga gtattagtta cgcttgacag
    38161 agtgtaaaac aaaacattta aatcataacg acaaataatt ttgcggagag cactgtggat
    38221 acggaattgt taaaaacttt cctggaagtt agccgaacgc gtcactttgg tcgagcggct
    38281 gaatcgctct atctgaccca gtcagcagtg agctttcgaa tcagacaact ggaaaatcaa
    38341 ctgggtgtga accttttcac ccgccacaga aacaatatcc gtttaaccgc tgccggtgaa
    38401 aaactactgc cttatgcaga aacgctcatg agcacctggc aggccgcccg taaggaggtg
    38461 gcgcatacct cacgacataa cgagttttct atcggtgcca gcgcctcatt gtgggaatgt
    38521 atgcttaatc agtggctggg acgtttgtat caaaatcagg atgcccatac aggcttacag
    38581 ttcgaagcgc gaattgccca acggcagtct ctggtaaaac agctgcatga acgccagctt
    38641 gatcttctta ttaccactga agcgcccaaa atggacgaat ttagtagtca gttgctgggg
    38701 tatttcactt tagcgcttta taccagtgcc ccttctaaac taaagggaga tcttaactat
    38761 ctgcgactgg agtgggggcc agattttcaa cagcatgagg caggtttgat cggtgctgac
    38821 gaagtgccca ttctgacaac cagttctgct gaactggcac agcaacagat tgcgatgctt
    38881 aatggttgca cctggctacc cgtcagctgg gcgcgtaaaa aaggcggcct gcataccgtt
    38941 gtcgatagca caacactttc acggccgctt tatgccatat ggctgcaaaa tagcgataaa
    39001 aatacgttga ttcgcgatct attgaaaatt aacgtgctgg atgaagtgta ttaatctgaa
    39061 tggctggcaa ggatgccggt agaaggattt acttcagaga gggttatttc agataaaaaa
    39121 aatccttagc tttcgctaag gatgatttct ggcaggggcg gagagactcg aactcccaac
    39181 acccggtttt ggagaccggt gctctaccaa ttgaactacg cccctaatta gggtggcgga
    39241 acggacggga ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa
    39301 ctaccgctcc accgaattct tttacaacca ccggttttat gaccggctt
//
LOCUS       DAZFDC010000027        38056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFDC010000027 DAZFDC010000000
VERSION     DAZFDC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.027"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(147..842)
                     /gene="aqpZ"
                     /locus_tag="ACHJ48_001571"
     CDS             complement(147..842)
                     /gene="aqpZ"
                     /locus_tag="ACHJ48_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO4707151.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1268..2926
                     /gene="ybjD"
                     /locus_tag="ACHJ48_001572"
     CDS             1268..2926
                     /gene="ybjD"
                     /locus_tag="ACHJ48_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO4707152.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQAHHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2923..3879)
                     /gene="ybjX"
                     /locus_tag="ACHJ48_001573"
     CDS             complement(2923..3879)
                     /gene="ybjX"
                     /locus_tag="ACHJ48_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO4707153.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTVMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4030..5145
                     /gene="macA"
                     /locus_tag="ACHJ48_001574"
     CDS             4030..5145
                     /gene="macA"
                     /locus_tag="ACHJ48_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO4707154.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5142..7088
                     /gene="macB"
                     /locus_tag="ACHJ48_001575"
     CDS             5142..7088
                     /gene="macB"
                     /locus_tag="ACHJ48_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO4707155.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPVQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFMTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7161..7385)
                     /gene="cspD"
                     /locus_tag="ACHJ48_001576"
     CDS             complement(7161..7385)
                     /gene="cspD"
                     /locus_tag="ACHJ48_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO4707156.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7535..7609
                     /gene="yljB"
                     /locus_tag="ACHJ48_001577"
     CDS             7535..7609
                     /gene="yljB"
                     /locus_tag="ACHJ48_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO4707157.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7708..8028
                     /gene="clpS"
                     /locus_tag="ACHJ48_001578"
     CDS             7708..8028
                     /gene="clpS"
                     /locus_tag="ACHJ48_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO4707158.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8059..10335
                     /gene="clpA"
                     /locus_tag="ACHJ48_001579"
     CDS             8059..10335
                     /gene="clpA"
                     /locus_tag="ACHJ48_001579"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO4707159.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10681..10768)
                     /locus_tag="ACHJ48_001580"
     tRNA            complement(10681..10768)
                     /locus_tag="ACHJ48_001580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10732..10734),aa:Ser,seq:gga)
     gene            complement(11022..11240)
                     /gene="infA"
                     /locus_tag="ACHJ48_001581"
     CDS             complement(11022..11240)
                     /gene="infA"
                     /locus_tag="ACHJ48_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO4707160.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11525..12229)
                     /gene="aat"
                     /locus_tag="ACHJ48_001582"
     CDS             complement(11525..12229)
                     /gene="aat"
                     /locus_tag="ACHJ48_001582"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO4707161.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRNYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12271..13992)
                     /gene="cydC"
                     /locus_tag="ACHJ48_001583"
     CDS             complement(12271..13992)
                     /gene="cydC"
                     /locus_tag="ACHJ48_001583"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO4707162.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDS
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKQLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13993..15759)
                     /gene="cydD"
                     /locus_tag="ACHJ48_001584"
     CDS             complement(13993..15759)
                     /gene="cydD"
                     /locus_tag="ACHJ48_001584"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO4707163.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELASTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15882..16847)
                     /gene="trxB"
                     /locus_tag="ACHJ48_001585"
     CDS             complement(15882..16847)
                     /gene="trxB"
                     /locus_tag="ACHJ48_001585"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO4707164.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17392..17886
                     /gene="lrp"
                     /locus_tag="ACHJ48_001586"
     CDS             17392..17886
                     /gene="lrp"
                     /locus_tag="ACHJ48_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO4707165.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18021..22049
                     /gene="ftsK"
                     /locus_tag="ACHJ48_001587"
     CDS             18021..22049
                     /gene="ftsK"
                     /locus_tag="ACHJ48_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO4707166.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDENIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLSPPPFD"
     gene            22204..22815
                     /gene="lolA"
                     /locus_tag="ACHJ48_001588"
     CDS             22204..22815
                     /gene="lolA"
                     /locus_tag="ACHJ48_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO4707167.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22826..24169
                     /gene="rarA"
                     /locus_tag="ACHJ48_001589"
                     /gene_synonym="mgsA"
     CDS             22826..24169
                     /gene="rarA"
                     /locus_tag="ACHJ48_001589"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO4707168.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24260..25552
                     /gene="serS"
                     /locus_tag="ACHJ48_001590"
     CDS             24260..25552
                     /gene="serS"
                     /locus_tag="ACHJ48_001590"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO4707169.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25791..28235
                     /gene="dmsA"
                     /locus_tag="ACHJ48_001591"
     CDS             25791..28235
                     /gene="dmsA"
                     /locus_tag="ACHJ48_001591"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO4707170.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSGEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28246..28863
                     /gene="dmsB"
                     /locus_tag="ACHJ48_001592"
     CDS             28246..28863
                     /gene="dmsB"
                     /locus_tag="ACHJ48_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO4707171.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLMPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28865..29728
                     /gene="dmsC"
                     /locus_tag="ACHJ48_001593"
     CDS             28865..29728
                     /gene="dmsC"
                     /locus_tag="ACHJ48_001593"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO4707172.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29764..30390)
                     /gene="ycaC"
                     /locus_tag="ACHJ48_001594"
     CDS             complement(29764..30390)
                     /gene="ycaC"
                     /locus_tag="ACHJ48_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO4707173.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30705..31853
                     /gene="ycaD"
                     /locus_tag="ACHJ48_001595"
     CDS             30705..31853
                     /gene="ycaD"
                     /locus_tag="ACHJ48_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4707174.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32063..33493
                     /gene="ycaM"
                     /locus_tag="ACHJ48_001596"
     CDS             32063..33493
                     /gene="ycaM"
                     /locus_tag="ACHJ48_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4707175.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYTPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33494..33604)
                     /locus_tag="ACHJ48_001597"
                     /pseudo
     CDS             complement(33494..33604)
                     /locus_tag="ACHJ48_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(33703..34443)
                     /gene="pflA"
                     /locus_tag="ACHJ48_001598"
     CDS             complement(33703..34443)
                     /gene="pflA"
                     /locus_tag="ACHJ48_001598"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO4707176.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34635..36917)
                     /gene="pflB"
                     /locus_tag="ACHJ48_001599"
     CDS             complement(34635..36917)
                     /gene="pflB"
                     /locus_tag="ACHJ48_001599"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4707177.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(36972..37829)
                     /gene="focA"
                     /locus_tag="ACHJ48_001600"
     CDS             complement(36972..37829)
                     /gene="focA"
                     /locus_tag="ACHJ48_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO4707178.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9424 a         9323 c        10083 g         9226 t
ORIGIN      
        1 gtcctgtaag acgcgccctc agcccacatc catgtgggct gccccggcct tcggggaacg
       61 cttcagcgat tttgacgcca ccaatacccg tgcggtttgt aatgtcgaag ataaaacaac
      121 cagggcagat aaaatgccaa tgctaattaa ttacgctttt ccagcagagt ccggtaaatc
      181 agaccaccga taatgccacc gacaattggc accacccaga agaaccacag ttgttctaat
      241 gcccagccgc cctggaagat ggcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta
      301 gtcaccggaa tactgattaa gtgaatcagg gttaaggcca gaccaatagc gatcggcgca
      361 aaacctgccg gagcgaattt gtcggttgca ccgtggatca ctaacaggaa gcccgcactc
      421 aataccagtt caactaccag cgcggaaagc atggaataac cgcctggtga atgctcgcca
      481 taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac ccgttttacc actggcaatt
      541 aaatacagca gcgctgccgc aacaataccg ccgacaacct gggcaattac gtagccaacg
      601 acttcttttg ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc cgggttaaaa
      661 tgaccaccag aaatatgacc aacagcaaag gccatcgtca gaacggtcag accgaacgcc
      721 aacgccacgc cggcaaaacc aatgcctaat tccgggaagc ctgcggccag tacagcacta
      781 ccacagccac caaaaacaag ccagaaagta ccaaaacatt cagctgctaa ttttctgaac
      841 atatccacct caattaaaaa ttgaccctgt gaaaaatatg gtcgttttat agggtcgtcg
      901 taaaaaagtg acgacggaaa taatgcgcgg ctattttaaa aacgaaggcg agtcattcac
      961 cagataaata aatccagtaa atttgattta gggcaacagc gggttgcccc atatagtcat
     1021 ttgtctgatt gacagtgtag tgcaagcaaa agatttaatc ctttaggcgt aataaaaaat
     1081 aatttatcat gctaattatt tgattttgtt gtttttgcag acttatcagc aagagggagt
     1141 ataacgcgat tattcgctca tttttcagac atttgccatg cttaaatgtg atgtcatcac
     1201 gtattagcaa ggcctttccc gttatactgc cagcgtaaag gataagtcac atatttctgg
     1261 aggggatatg attcttgagc gcgttgaaat tgtgggtttt cgcggtatca accgtttgtc
     1321 gttgatgctg gaacaaaaca acgtcctgat tggggagaac gcgtggggta aatccagctt
     1381 gctggacgcc ttaaccctgc tgctatcgcc agaatcagat ctctaccatt ttgagcgcga
     1441 cgatttctgg ttcccgccgg gagatatcaa cgggcgagaa catcatctgc atattatttt
     1501 gaccttccgc gaatcgctgc caggtcgaca tcgggttcgc cgttatcggc cgctggaagc
     1561 gtgctggacg ccatgcaccg atggctatca ccgtattttt tatcgtctgg aaggggagag
     1621 tgcggaagac ggcagcgtga tgacactgcg cagttttctc gataaagacg gacatccgat
     1681 tgatgttgag gatattaacg atcaggcaca ccatctggtg cgtttaatgc cggtgctgcg
     1741 cttgcgtgat gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa atgtccccaa
     1801 tgtggaagtc accgcgcgcc agctcgattt cctcgcccgt gagttgtcct cacatccgca
     1861 aaatctctct gatggacaga ttcgtcaggg actttccgca atggtgcagt tgcttgagca
     1921 ttatttctct gagcaggggg ctggacaggc gcgatatcgt ttaatgcggc ggcgggccag
     1981 caatgagcaa cgaagctggc gctatctgga catcatcaac cggatgattg accgacctgg
     2041 agggcgctcg tatcgggtta ttttgctcgg cctgtttgct actttattgc aggcaaaagg
     2101 cacattgcga ctggataaag acgcccgtcc attgttgctg atcgaagatc cagaaacgcg
     2161 tttacacccc attatgcttt cagttgcctg gcatctgttg aatcttttgc ctttacaacg
     2221 tattgctacc accaactctg gcgagctgct ttcgttaact ccagtggaac atgtttgccg
     2281 cctggtgcgt gaatcttctc gtgttgccgc ctggcgtctg gggccaagtg gcttgagcac
     2341 cgaagacagc cgccgcatct cttttcacat tcgttttaat cgtccgtcat cgctgtttgc
     2401 tcgctgctgg ttgctggtgg aaggggaaac ggaaacctgg gttatcaatg aactggcgcg
     2461 tcagtgcgga catcattttg atgccgaagg gatcaaggtc attgagtttg cccagtccgg
     2521 gctaaagcca ctggttaaat ttgcccgccg aatggggatt gaatggcatg tactggtcga
     2581 tggcgatgaa gcagggaaga aatatgccgc tacggtacgc agcctgttga ataatgatcg
     2641 ggaagccgaa cgagaacatt taacggcgtt accggcgctg gatatggaac attttatgta
     2701 tcgccaggga ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa atgtcccgat
     2761 gaatctgcgc aaaattatct cgaaagcgat ccatcgctct tccaaacccg atcttgccat
     2821 tgaagtggca atggaggccg gacgtcgtgg tgtggactcc gtaccgacgc tgctgaaaaa
     2881 aatgttctca cgcgtgctgt ggctggcgcg cggtcgcgcg gattaaccgc gaaacatcgt
     2941 ggccatttgt ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt cagcccgttt
     3001 tttactggcg atttcggcaa tctcttttcg tgctatctgt gctggaaggc ggtaatggcg
     3061 ttcagcatca catacgccgc caaccgattc ccagaaagcg ttgtaatcgg catggatctt
     3121 gccttcttta tcgcgataac gcaggctgcg gtaaatatgc gtttcattgc tgacggcaat
     3181 aatctgctct acctgcaaac gttgggcaaa cagacaggcc gcttccatca cgaggcgttt
     3241 gggaaatagc ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg gaatttccca
     3301 ttttgcgcct tgcagtccac caataaacat cgttcttttc ccctgatatt cacacagggt
     3361 aaacgtgatt tctgccagag gaataccttc gctgttgcgg aacaggattg tgctgtcacc
     3421 ttctttatcc attgagatca tcacggtcag ctcaagcgtg aactgctcgc cgtttttgcc
     3481 ttccagcttc gccagttgca gcccgggggt attcaaatat aagctgaatt cttccgccga
     3541 catacatcca cggagtaacg cataatggta acgtaacgcc tccagcaatt gcttacggct
     3601 aagattcgcc gcaaggtaag ggcgatgcag acgcacaggc agtcgcggct ggcgcgttaa
     3661 caatacattg agattaggcc agtgggaaag ttcgttcatc cactcaacgc ttaaacgcgg
     3721 cataatcaac gagcgcagca aaaatttctg gcgaaaacta cggcgatgcc agaatttacc
     3781 cggccgacac tgtccacgtg ccagactaag aaaaagtgac aggctgctga gagattcaga
     3841 tgacgtaaag gtccgttcgg ttagctgcga catattcatg aaatcaatgg ttgtacatga
     3901 cgtcgatttc accattgcgt atcttaacca aacatcaata gtgtgattac taacgtaaat
     3961 tttagggttt tgttgatatt tcgttgaagt taatgacccg gattggcata tggagtattc
     4021 agaatattta tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc gctggtgata
     4081 gtcatcgccg gactgattac gttatggaga attcttaacg cacccgtgcc gacttatcag
     4141 acactgattg tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg aaagctggac
     4201 gcgctgcgta aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac gctgtcggtg
     4261 gcgattggcg ataaagtaaa aaaagaccag cttttagggg ttattgatcc tgaacaggct
     4321 gaaaaccaga tcaaggaggt cgaagcaacg ctgatggagc tacgcgcgca gcggcagcag
     4381 gcggaagcgg agctgaaact ggcgcgggtg acgtattccc gtcagcaacg tctggcacaa
     4441 acgcaggcgg tttcactgca ggatctcgac accgccgcga cggagatggc tgtgaaacag
     4501 gcgcaaattg gcaccattga cgcgcaaatc aagcgcaatc aggcttctct cgatacggct
     4561 aaaaccaatc tcgattacac tcgcatcgtt gccccgatgg ccggggaagt cacgcaaatc
     4621 accactctgc aaggccagac ggtgattgcc gcacaacaag caccgaacat tctgacgctg
     4681 gcagatatga gcaccatgct ggtaaaagcg caggtttctg aagcggatgt aatccacctg
     4741 aagccggggc aaaaagcctg gtttacggta cttggcgatc cactgacgcg ctacgagggg
     4801 caaatcaagg atgtactacc gacgccggaa aaggttaacg acgctatttt ctattacgcc
     4861 cgttttgaag tccccaaccc caatggtttg ctgcggctgg atatgactgc gcaagtgcat
     4921 attcagctca ccgatgtgaa aaatgtgctg acgatccctc tgtcggcgtt aggcgatccg
     4981 gttggcgata atcgttataa agtcaaattg ttgcgtaatg gtgaaacacg cgagcgtgaa
     5041 gtgacgattg gcgcacgtaa cgataccgat gttgagattg tcaaagggct ggaagcgggc
     5101 gatgaagtgg tgattggtga ggccaaacca ggagctgcac aatgacgcct ttgctcgaat
     5161 taaaggatat tcgtcgcagc tatcctgccg gtgatgagca ggttgaggtg ctgaagggca
     5221 tcaccctcga tatttatgcc ggtgagatgg tagcgattgt tggcgcttcg ggttccggta
     5281 aatcgaccct gatgaatatt ctcggctgtc tggataaggc caccagcggc acctatcgcg
     5341 tcgccggtca ggatgttgcc acgctggacg ccgatgcgct ggcgcaactg cgccgcgagc
     5401 atttcggctt tattttccag cgttaccatt tgctttcgca tttaaccgcc gagcagaacg
     5461 ttgaagtacc agccgtctat gctggtcttg agcggaaaca gcggctgctt cgcgcccagg
     5521 agttgctgca acgactgggg ctggaagacc gtacagagta ttatccggta cagctttcgg
     5581 gtggtcagca acagcgcgtt agcatcgcgc gggcattgat gaacggcggt caggtaattc
     5641 ttgccgatga accgaccggc gcactggaca gccattcagg cgaagaggtg atggcgatcc
     5701 tgcatcagct gcgcgatcgc gggcatacgg tgattatcgt cactcacgat ccgcaggtcg
     5761 ctgctcaggc cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc aatcctcccg
     5821 ccattgaaaa agtgaatgtt gctggcggga cggaacctgt tgtcaacacg gcgtctggct
     5881 ggcggcagtt tgtcagcggt tttaatgagg cgctgacgat ggcatggcgg gcgctggcag
     5941 cgaataaaat gcgtacttta ctgaccatgc tggggattat aatcggtatt gcgtcggtgg
     6001 tttccattgt cgtggtgggc gacgccgcca aacaaatggt gctggcggat attcgttcta
     6061 ttggtacgaa tactattgat gtctatcccg ggaaagattt tggcgatgac gatccgcaat
     6121 atcaacaggc gctgaagtac gatgacttaa tcgccatcca aaaacaaccg tgggtcgctt
     6181 cagccacacc tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt gatgttgctg
     6241 ccagtgccaa tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc ttcagtgaag
     6301 gaaacacctt taaccaggaa cagctgaacg gtcgtgcgca ggtcgtggtt ctcgacagta
     6361 atactcgccg ccagcttttc ccccataaag cagatgtggt tggcgaggtg attctggtcg
     6421 gcaatatgcc cgccagagtc attggtgtgg cggaagaaaa acagtcgatg tttggtagca
     6481 gtaaagtgct gcgtgtctgg ctaccttaca gcacgatgtc cgggcgagtt atgggccagt
     6541 cgtggctgaa ctccattact gtcagggtga aagaaggatt tgacagcgcc gaggcggaac
     6601 agcaactcac gcgtttactt tcactgcgcc acggtaagaa ggatttcttt acctggaaca
     6661 tggacggcgt cttgaaaacc gttgaaaaga ccacacgtac tttacaactg tttatgacgc
     6721 tggtggcggt gatttcgctg gtagtgggcg gtattggtgt aatgaatatt atgctggtgt
     6781 cagtgaccga gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg cgagcaagcg
     6841 atgttttgca acagttcctg atcgaagccg tactggtttg cctggtcggt ggcgcgttgg
     6901 gaataacact gtcactgtta attgctttca ccttgcagct tttcttaccc ggctgggaga
     6961 ttggtttttc accgttggca ctgctgctgg cgtttctctg ctcgacggtc accgggattt
     7021 tatttggctg gttacccgca cgaaatgcgg cacgactgga tccagtagat gctctggcac
     7081 gagagtaatt tttgagataa aaatgccagc cgatcgggct ggcattttgc ctttaggatg
     7141 tacacaatga gacagaagag ctatgcgact gccgcttcta cttcgacggg cacaataaca
     7201 ctggcgtgat tgccttttgg cccctggtgg acatcaaact gaacggattg tccagctttt
     7261 agcgttctgt aaccatccat ctgaatggtg gaataatgag cgaaaatatc ttcgccgccg
     7321 ccttcagggc agatgaaacc aaaccctttg gcattgttga accacttaac agtacccttt
     7381 tccatgcttc gacatccttc gcaaatctta tacaagtaag atggaataaa ccggggtcag
     7441 agagggggct gttcaaaacc tcgccaactc tagaaataca atttagagaa ttagggcgag
     7501 ccgtcaagca tttgacaggg gacagggggc aggtatgaat caaaaatttg aagcagttaa
     7561 cgctattgac aggaatgtga cagatgtcgc tgatgccaac gatagatgat agttatctat
     7621 catgtggagt agattggtca ggcaaataag ctcttgtcag cggcagggcg ttctgccgat
     7681 aaccgtaacc gaagatgata actgacaatg ggtaaaacga acgactggct ggactttgat
     7741 caactggcgg aagaaaaagt tcgcgacgcg ctaaaaccgc catctatgta taaagtgata
     7801 ttagtcaatg atgattacac tccgatggag tttgttattg acgtgttaca aaaattcttt
     7861 tcttatgatg tagaacgtgc aacgcaattg atgctcgctg ttcactacca ggggaaggcc
     7921 atttgcggag tctttaccgc cgaggttgca gaaaccaaag tggcgatggt gaacaagtac
     7981 gcgagggaga atgagcatcc attgctgtgt acgctagaaa aagcctgaat gcaggcataa
     8041 aaattggggg aggtgcctat gctcaatcaa gaactggaac tcagtttaaa tatggctttc
     8101 gccagagcgc gcgagcaccg tcatgagttt atgaccgtcg agcacttgtt actggcgctg
     8161 ctcagtaacc catctgcccg ggaggcgctg gaagcgtgtt ctgtggattt ggttgcgctc
     8221 cgtcaggaac tggaagcctt tattgaacaa accacacccg ttctgcctgc cagtgaagag
     8281 gagcgcgaca cacagccgac gctgagtttt cagcgtgtac tgcaacgtgc ggtcttccat
     8341 gtccagtcct ccggtcgcaa tgaggttacc ggtgcaaacg ttctggtcgc tatctttagc
     8401 gaacaggagt cgcaggcggc atatctgttg cgtaaacatg aagtcagccg tctcgatgtg
     8461 gtgaacttta tctctcatgg cacgcgtaaa gacgagccga cacagtcttc tgatcctggc
     8521 agccagccaa acagcgaaga acaagctggt ggggaggaac gtatggagaa tttcacgacg
     8581 aacctgaatc agcttgcgcg cgtgggcgga atcgacccac tgattggtcg tgagaaggag
     8641 ctggagcgtg ctattcaggt tctctgccgt cgccgtaaaa acaacccgct gctagtgggg
     8701 gaatctggtg tcggtaaaac cgcgattgca gaaggtcttg cctggcgaat tgttcagggc
     8761 gatgtgccgg aagtgatggc tgactgtacg atttactctc tcgatatcgg ttctctgtta
     8821 gcgggcacaa aatatcgcgg cgactttgaa aaacgtttta aagcgttgct caagcagctg
     8881 gagcaggaca ctaacagcat cctgtttatt gatgagatcc acaccattat cggtgcgggt
     8941 gcagcgtctg gtggtcaggt tgatgcggct aacctaatca aaccgttgct ctccagcggt
     9001 aaaattcgcg taattggttc gacaacctat caggagttca gcaacatttt cgagaaagac
     9061 cgtgctctgg cgcgtcgctt ccagaaaatt gatattactg aaccgtcgat cgaagaaact
     9121 gttcaaatca tcaatggcct gaaaccgaag tatgaagcac accacgacgt gcgttatacc
     9181 gcaaaagcgg tgcgtgcggc ggtagagctg gcggtgaaat acattaacga tcgtcatctg
     9241 ccggataaag ccattgatgt tatcgacgaa gcgggcgctc gcgcacgtct gatgccggta
     9301 agcaaacgca agaaaaccgt taatgtggcg gatattgagt ccgtggtggc ccgtattgca
     9361 cgcattccag agaagagtgt ttctcagagt gaccgcgata ccctgaaaaa cctcggcgat
     9421 cgcctgaaaa tgctggtctt cggtcaggat aaagccattg aggcgctgac tgaagccatt
     9481 aagatggcgc gtgcaggttt aggtcacgaa cataaaccgg tcggttcgtt cctgtttgcc
     9541 ggccctaccg gggtcgggaa aacagaggtg acggtacagc tttcgaaagc tttgggcatt
     9601 gagcttctgc gctttgatat gtccgagtat atggaacgcc ataccgtcag ccgtcttatt
     9661 ggtgcgcctc cgggatacgt tggttttgat cagggcggtt tgctgactga tgcggtcatc
     9721 aagcatccac atgcggtgct gctgctggac gaaatcgaga aagcgcaccc ggacgtgttc
     9781 aatattctgt tgcaggtgat ggacaacggt acgctgaccg ataacaacgg acgcaaagcg
     9841 gacttccgta acgtggtact ggtgatgacc actaacgccg gggtacggga aactgagcgc
     9901 aaatccattg gtcttatcca ccaggataac agcaccgatg cgatggaaga gatcaagaag
     9961 atctttacac cggaattccg taaccgtctc gacaacatta tctggttcga tcatctgtca
    10021 actgacgtga tccatcaggt ggtggataaa ttcatcgtcg agttgcaggt tcagctggat
    10081 cagaaaggtg tttctctgga agtgagccag gaagcgcgta actggctggc cgagaaaggt
    10141 tacgaccggg caatgggcgc acgtccgatg gcgcgtgtca tccaggacaa cctgaaaaaa
    10201 ccgctcgcca acgaactgct gtttggttcg ctggtggacg gcggtcaggt caccgtcgcg
    10261 ctggataaag agaaaaatga gctgacttac ggattccaga gtgcacaaaa gcacaaggcg
    10321 gaagcagcgc attaatctga ttgtcaggta ggttggtgaa gtccgtaatc tcgaaagagg
    10381 ttacggactt tttatttatg gggtggaacg gtgaaaaccc tatttttgga ggtgaaggta
    10441 atttgttgat aattagtgct gctggaaaag gtaaggataa aaaagggtga cagcaggaaa
    10501 atgagatggt tttgctttat taacaacggg ctaaacgtgt agtatttgag ttcactgccg
    10561 tacaggcagc ttagaaattc actggtaaca tactccaccc gcccaccatg ttcactgccg
    10621 tacaggcaga taaaatgcga aaaaaaagcc cgtactttcg tacgagctct tctttaaata
    10681 tggcggtgag ggggggattc gaacccccga tacgttgccg tatacacact ttccaggcgt
    10741 gctccttcag ccactcggac acctcaccaa attgttttgc tgccaaacct catgggtggc
    10801 aacggggcgc tactataggg agttggagta aaacggtcaa gaagaatttt aatgataatt
    10861 attgtttgct catactgtaa acaacttgtg cagtatatct acatcgagac aagttatgga
    10921 cttatacttc caaagtactt catgcatatc acaaaataaa aaggccggtt aaaccgacct
    10981 tttactcgtt ctttctcttc gcccatcagg cggtaaaaca atcagcgact acggaagaca
    11041 atgcggcctt tgctcaggtc gtacggggtc agttcaacag tcactttgtc gcccgtcagg
    11101 atgcggatgt agtttttgcg cattttaccg gagatgtgtg cagtaaccac gtgaccgttt
    11161 tctaactcta cgcggaacat ggtattaggc aacgtttcaa gaacggtacc ttgcatttca
    11221 atattgtctt ctttggccat ctaatcctct ggggtatcac taccgtaatt tgaaccggca
    11281 agataatgcc gaagttctgt aaataagtaa agatttgcgc gctaaatcgc aacaaacagg
    11341 ttcggcacat tactccgaaa acacacggct aagccgcacc aaaagcgcaa cgtataaggg
    11401 agcggtgaga taaacgatgg gcgttacctg acgcgaaaaa ttccttatcg gcagcggggt
    11461 aatgagcgta accaactctg cgaccgcaat tataacactc tggggagaaa tgtgccgaaa
    11521 acattcattc ttgtggtgaa aacaagcatc gtggtaccca gaaattattc ggcaatcgtc
    11581 cgaggcgcat ttgattgaga taattaaggt aattccggcg gggaatttcg caggcaccaa
    11641 gcgatgctgt gtgatcgtta aggacctggc agtcgataag cttaccgcca tgaccgataa
    11701 attcctcaca gaataccaga agcgccgttt tagacgcatt ttccatccgg ctgaacatgg
    11761 actcgccaca aaatagcgtt ccctgggcca cgccgtacat accgccgaca agctcatctt
    11821 cacgccagac ttcaatggag tgggcatgac cgagttcgtg aaggcgatgg taggcttcga
    11881 ccacgccacg cgtgatccag gttccttctt cgcgatcgct ggcacagcct tcaatgacct
    11941 gaccaaaagc gtaattcatc gtgacacgat agggcgagcg tttatgaaat cgcttcatac
    12001 tacggctgat atgcagtgat tctggccata gcaccgcgcg gggatcgggc gaccaccaga
    12061 ggatggggtc gcctggagaa aaccacggaa aaataccacg ctggtaagcc attaacaggc
    12121 gcgcagggct aagatcgccc ccaagtgcca gcaggccgtt aggctcacgt aatgcgcctt
    12181 ccggggaagg gaaggctatt gaatggcgag aaagctgaac caggcgcatg accgcaaaac
    12241 tccacgcaag tcggatcgtt caataatagc ttacaaaccc tgcttgaact ggtaataacg
    12301 cccctgtctg gcaagcagtt ctgcgtgagt accttgctca ataatttgcc cgttgtccat
    12361 cactattatt tgttggaaac gagagagtcc gcgaagtcga tgggtgacca ttaacaccgt
    12421 tttctcacgc atcatttctg caagcaattc aaggatctgg ctttcggttg tggcatctaa
    12481 gccttcggta ggttcatcca gcaacaccag tggcgcatca tgtaacagcg cacgggcgat
    12541 agccagacgg cgcagttcac caccggagag ctggcgtccg ccttcaccta accaactgtt
    12601 gagacctgca tcctcgagct gcttctccag accaacgcga cgtaagatca ccgccagagc
    12661 ctcatcacta ctgccaggcg aggcgagtaa cagattatca cgcagcgtgg cgctaaacag
    12721 atgcactcgc tgaggaacaa cgctgatggt ctgtcgtaga gccgcttcat tcaggctggc
    12781 tatggggcta tcgttaagca aaatctcgcc ctgttgcggg tcccatgcgc gggtcagcag
    12841 ttgtaacagt gttgatttgc cgcatccggt tcgcccgaga atcgctatat gttccccggc
    12901 gtttacctga agagaaatcc ctttaagtgc ctgttgggat tgctccggat aagtgaactg
    12961 aacatcccgt aatgtcagcg aaacgcgatc ggcaacacga gtttgggtat cagggaaggt
    13021 gacctccggt ttttgatccg ttaagtcaga gatacgtacg gcagaggcaa tgacttgccc
    13081 cagatgctga aatgcacccg ttactggtgc cagtgcttca aacgcggcta acgcgcaaaa
    13141 gacaaacagg gcaattaacg cgccgggttg agcattgccg ccaacgccgc cagacgccat
    13201 ccacagcatc aggatcaccg ctaacgcgcc aatgagcagc attatcgctt gcgacaatgc
    13261 ggtcagttca gattgacggc gttgcgcttc cagccattga atttctgtat tctctagttg
    13321 cgtgcgataa cgatcgctgg caccaaaaat ggtcagctca gcttgccctt gcagccaggc
    13381 cgtcagttgt tggcgatact gtccgcgaag atgagtcaga ttttgcccgg tgctttttcc
    13441 cgcacgataa aacagcggtg gcatcaggaa aagcgtcagt aacataatgc cgcccagcgt
    13501 aaaggcgagg gtggaatcaa ggaaacttaa cccgattgtc accaccataa tcaccacaaa
    13561 agcgcccacc agcggcgaga taacgcgcag gtaaagatga tcgagcgtat caacatccgc
    13621 caccacgcgg ttgagcaatt cgccctgacg atagcgcgcc agtccggcag gggagagggg
    13681 caacaatttg ctgaaggtgt aaatgcgcag atgctgcaac acgcggaaag tcgcgtcgtg
    13741 acttaccaga cgttcaaaat aacgcccggc agtacgggtg attgctgcac cacgcacgcc
    13801 cgcagcgggt agcatatagt tgaagctgta cagtccggca acccccgcaa ccgctgaggc
    13861 cgagaggaac cagccggaaa gtgtcaacag accgatactg gcgagcagcg tcacaattgc
    13921 cagcacaata ccaagactta acatccattt atgacgttta tacagtgcca gatagggtag
    13981 caaagcgcgc atttaaatct cctcctgacg atgggccagt aatgtggcga atgggccacc
    14041 agccacactt aattccgcgt aacgtccttg ctcaataatc cggccatcct gcataaccca
    14101 aatgacatcc cagtcagcaa gatcttctaa ctggtgggtg accattaacg ttgtctggcg
    14161 cagagaggcg gcattcagcg cctccattac gcgctgttca ctgtgagcat caaggctggc
    14221 agcgggttca tccaacagta atagcgaaca gggatttagt aacgcacggg ccaccgccac
    14281 gcgctgcgcc tgccccacgg aaaggcgggc agcctggtcg ccaacaggcg tatcaacgcc
    14341 ttgtggcagg agcggtagaa actcgctgac ccaggcgtta tccagcgctg cttgtaattc
    14401 ttgttcgctg gcatcaggtc gcgccagtag tacgttatcc cgtaatgttg ctgccggtaa
    14461 ttgtgggttt tgaccaaccc aggagagatg tttacgccat gattctggtg ataaatcgcg
    14521 taattctatc ccgttgattc gtaatgatcc ctgatatgag agaaaaccag aaagcgcgtt
    14581 cagcagtgag cttttacctg aaccgctgcg accaaccaac actgcacgtt ggcctgctgg
    14641 caaagtaaag ttcagcggtc cggccagcgt tttaccttcc ggcgacgtga taaacagatc
    14701 ctcggcctca atggtcagcg gatcggttga tgctaattcc gtctcaccgc gctgcggatg
    14761 ggcgagcggg gtttccataa acgttttcag actgtcagct gcaccaacag cctgggcttt
    14821 agcatgataa aacgtaccga gatcgcgtaa tggctggaaa aactctggcg caaggatcag
    14881 ggccagaaaa cccgcagcca gcgtcacgcc agtatcgtag tgaccaaaat ccagctcgcc
    14941 gagataggaa aaaccaaagt agaccgccac cagagcaatt gacagcgagg taaaaaattc
    15001 gagaatgccg gaggataaaa acgccagccg tagcacttcc attgtccgtt ggcggaaatc
    15061 ttccgaagca gaacgaatac tttcaatttc agcttcacca cgaccaaaaa tacgcaatgt
    15121 ttccatgccg cgcaggcgat cgaggaaatg cccacttaag cgagcaagag cgagaaagtt
    15181 acgtcggtta gcatcggcag cccccatccc aaccagcgcc ataaacaacg gaattaacgg
    15241 tgcagtgccc agcagaatga gcgccgcagc ccagttagag gggaagatcg ccaccacaat
    15301 cagcaacggc accgacactg ccagcgccat ttgcggcagg tagcgtgcat agtaatcatg
    15361 catatcgtca atttgctcga gtaccagcgt cgcccagctc cccgcaggtt taccctgaat
    15421 ccacgctggc cctgcttgtt gcagacggtc gagaacctga cggcggatgg caaagcggat
    15481 atgctgcccg gcgtgataac ccacccgttc gcgtaaccag accacccatg cgcgcagtac
    15541 aaaggtcaga accagtaacg taaagggaag cagcagggct tcacggggaa tattctccat
    15601 aatcatatgt tgcagaattc gcgccatgaa ccatgcctgg gcaatgatca atatgccgct
    15661 cacaaagccc agcagacgag aaatattcag ccaacgttgg gagatgacgc tttgctgttt
    15721 taaccagcgg gttaactctt tttgacgaga tttattcatt gcacgcttag caggtgagtt
    15781 atcagaatta tttgcagagc aatgttacaa cggggaaaaa ataaaggcga cccatagtcg
    15841 catggtgtcg ccttctttac ttttgttact gatttgtaaa attattttgc gtcagctaaa
    15901 ccatcgaggt agcgttccgc atcaagtgct gccatgcagc ctgtaccagc agatgtaatg
    15961 gcctggcgat aaatgtgatc catcacgtcg cctgcggcaa agacgccagg aatgctggtc
    16021 tgggtggcat taccatgaat acccgactgc actttgatgt agccgttttc cagttccagc
    16081 tgcccttcga aaatggcagt attcgggctg tgaccgatag caacaaacag accggcaacg
    16141 tcgagtgact cgatgttatc gctgttttgc gtatcgcgca gacgaacgcc agtgacaccc
    16201 atttgatcgc cggtcacttc ttccagcgta cggttggtgt gcagaatgat gttgccgttc
    16261 tccactttat ccatcagacg cttaatgagg attttttccg cgcggaaacc gtcacggcgg
    16321 tgaatcagat gcacttccga agcgatgtta gacagataca gcgcctcttc aaccgcggta
    16381 ttgccgccgc cgatgaccgc aactttctgg ttgcgataga agaaaccgtc gcaggttgca
    16441 caagcagaaa ctccacggcc tttaaaggct tcttcagagg gcaggccgag atagcgtgca
    16501 gaagctccgg tggcaataat cagcgcgtcg caagtgtatt cgccgttatc gccattcaga
    16561 cggaacggac ggttttgcag atccaccttg ttgatatgat caaaaatgat ctcagtttca
    16621 aacttggtgg catgttcgtg catgcgctcc attaataacg gaccggtcag atcgtttgga
    16681 tcgccaggcc agttttccac ttccgtggtg gtggtcagtt ggccgccttt ttccatgccg
    16741 gtaatcagca caggttgcag gttggcgcgc gccgcataga cagcagcggt gtatcccgcc
    16801 gggcctgaac ccaggataag cagtttactg tgtttggtcg tgcccatgag atccccatag
    16861 ttgttggcag acaatgggca ggattgtagg gaatttacag acgtaaaaaa agagtatgac
    16921 gattttgtta acaatttgtg caatcggcag catcgataag caggtcaaat tctcccgtca
    16981 ttatcacctc tgctacttaa atttcccgct ttataagccg attaaatgat gaataaacgc
    17041 ccctgttaat gaatatctgg catgttgtac taaaaatcga tgttttgctt tgacaatccc
    17101 ctggtgtttt gcgaaaacat tcgaggaaga aaaaaaacag tattcttata tgcgcataac
    17161 catgcatgta aataccatgt ttaccgtgct agtgaaatct acgtatggcg tggacagacg
    17221 ccattcgtga tgtcgatagc tgccacaagg caacggtctt ctcaccgtag acccaggcat
    17281 tgcgcgccgt gaatcttcat gatttcggtc tatcgtgacg ggtagcgact ctgaacagtg
    17341 atgtttcagg gtcagacagg agtagggaag gaatacagag agacaataat aatggtagat
    17401 agcaagaagc gccctggcaa agatctcgac cgtatcgatc gtaacattct taatgagttg
    17461 caaaaggatg ggcgtatttc taacgtcgag ctttctaaac gtgtgggact ttccccaacg
    17521 ccgtgccttg agcgtgtgcg tcggctggaa agacaagggt ttattcaggg ctatacggcg
    17581 ctgcttaacc cccattatct ggatgcatca cttctggtat tcgttgagat tactctgaat
    17641 cgtggcgcac cggatgtgtt tgaacaattc aataccgctg tacaaaaact tgaagaaatt
    17701 caggagtgtc atttagtatc tggtgatttc gactacctgt tgaaaacacg cgtgccggat
    17761 atgtcagcct accgtaagtt gctgggggaa accctgctgc gtctgcctgg cgtcaatgac
    17821 acacggacat acgttgttat ggaagaagtc aagcagagta atcgtctggt tattaagacg
    17881 cgctaacacg gaacaggtgc aaaatcggcg tattttgatt acactcctgt taatccatac
    17941 agcaacagta ctggggtaac ctggtactgt tgtccgtttt agcatcgggc aggaaaagcc
    18001 tgtaacctgg agagcctttc ttgagccagg aatacactga agacaaagaa gtcacattga
    18061 caaagttaag tagcggccgc cgccttctgg aggcgttgct gatccttatt gtcctgtttg
    18121 ccgtctggtt gatggctgcc ttactaagct ttaacccttc ggaccccagc tggtcgcaaa
    18181 cggcctggca tgaacctatc cataatttag gtgggatgcc cggtgcgtgg ttggcagata
    18241 cgctgttctt tatttttggc gtgatggctt acaccattcc cgtcattatt gtcggcggtt
    18301 gttggtttgc ctggcgtcat cagtccagcg acgaaaacat tgattatttt gccgtttcgc
    18361 tacgcatcat tggcgttttg gcgctcatcc ttacctcctg tggtctggcg gcaatcaacg
    18421 ctgacgatat ctggtatttt gcctccggtg gcgtcattgg cagcttacta agcactacgc
    18481 tacaaccact gctacacagt agcgggggaa ctattgcgct gctctgcgtt tgggcagcgg
    18541 gcctgacgtt gttcaccggt tggtcatggg tgaccattgc tgaaaaactc ggcggctgga
    18601 ttttaaacat tctcaccttc gccagtaatc gtacccgtcg cgatgatacc tgggtcgatg
    18661 aagatgagta tgaagacgac gaagagtatg aagatgaaaa ccacggcaaa cagcatgaat
    18721 cacgccgtgc ccgtattctt cgcggcgcgc tagcgcgtcg taaacggttg gcggaaaaat
    18781 tcattaatcc gatggggcgg caaacagacg ctgcgttgtt ctccggtaag cggatggatg
    18841 atgacgaaga gattacctac actgcacgcg gtgtggctgc cgacccggac gacgtcctat
    18901 tttcgggcaa tcgtgcaacg cagccagaat atgacgaata cgatccatta ttaaacggtg
    18961 cgccaattac cgaacctgtc gctgtagcag ctgctgctac cacggcgaca caaagctggg
    19021 ctgcgccggt tgaacctgtg actcagacgc cgcctgttgc ctctgttgat gttccacctt
    19081 cgcaacctac agtagcctgg cagcctgtac cgggtccaca aacgggagag ccggttattg
    19141 ctcctgcacc ggaaggttac ccacagcagt cacaatatgc gcagcctgca gtgcaatata
    19201 atgagccgct gcaacaacca gtacagccgc agcagccgta ttatgcacct gcagctgaac
    19261 aacctgcgca acagccgtat tatgcccctg cgccagaaca accggtggca ggtaacgcct
    19321 ggcaagccga agagcagcaa tccacttttg ctccacagtc tacataccag actgagcaaa
    19381 cttatcagca gccagccgct caggagccgt tgtaccaaca gccgcaaccc gttgaacagc
    19441 agcctgttgt ggagcctgaa cccgttgtag aagagacaaa acccgcgcgt ccgccgcttt
    19501 actactttga agaagttgaa gagaagcgag cccgtgaacg tgaacaactt gcggcctggt
    19561 atcaaccgat tccagaaccg gttaaagaac cagaaccgat caaatcttcg ctgaaagcac
    19621 cttctgttgc agcagtacct ccagtagaag ccgctgccgc tgtttctccg ctggcatctg
    19681 gcgtgaaaaa agcgacactg gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca
    19741 gtctggcaaa tagcggtgga ccgcgtccgc aggtcaaaga ggggattggc ccgcagttgc
    19801 cacgaccgaa acgtatccgc gtgccaactc gtcgtgaatt ggcgtcttac ggtattaagc
    19861 tgccctcaca gcgtgcggcg gaagaaaaag cccgtgaagc ccagcgcaat cagtacgatt
    19921 ctggcgatca gtacaacgat gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc
    19981 agttcgccca gacacagcag caacgctatg gcgaacagta tcaacatgat gtgcccgtaa
    20041 atgcagaaga tgcagatgct gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc
    20101 aacaacaacg ttattccggc gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt
    20161 ttgaattttc gccaatgaaa gcgttgctgg atgatggtcc acacgaaccg ctgtttacgc
    20221 caattgttga acctgtacag cagccgcaac aaccggttgc accgcagcag cagtatcagc
    20281 agccacaaca gccggttgcg ccacagccgc agtatcagca gccacaacag caggttgcgc
    20341 cgcagccaca atatcagcag ccgcaacaac cggttgcgcc acagcagcaa tatcagcagc
    20401 cgcaacaacc agttgcgccg cagccgcagt atcaacagcc gcaacaaccg gttgcaccgc
    20461 agccgcagta tcagcagcca caacagccag ttgcgccaca gccgcaggat actctgcttc
    20521 atccgctgtt gatgcgtaat ggcgacagcc gtccgttgca taaaccgacg acgccgctgc
    20581 cttctctgga tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc
    20641 ttgaacaaat ggctcgcctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg
    20701 tcgtcaatta ctctccgggg ccggttatca ctcgctttga acttaacctg gcaccgggcg
    20761 taaaagcggc gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg
    20821 cggtgcgtgt cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccgaata
    20881 aaaaacgaca aaccgtttat ctgcgcgaag tattggataa cgccaaattc cgcgataatc
    20941 catcgccatt aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc
    21001 tggcgaaaat gccgcacttg ttagttgcgg ggactaccgg ttccggtaaa tctgtcggtg
    21061 tgaacgcgat gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca
    21121 tgatcgaccc gaaaatgctg gagctttcgg tgtatgaagg cattccgcac ctgttaacgg
    21181 aagtcgttac tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt aacgagatgg
    21241 agcgtcgtta taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa
    21301 aaattgctga agccgatcgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg
    21361 acagtatgga tgcccagcat ccggtgctga aaaaagaacc atacattgtg gtgttggtgg
    21421 acgaatttgc cgacctgatg atgacagtag gtaaaaaagt ggaagagctg atagcacgtc
    21481 tggcgcaaaa agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg
    21541 ttgatgttat tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat
    21601 ccagtaagat tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggta
    21661 tgggggatat gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt
    21721 ttgttcgcga tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac
    21781 agtatgttga tggcatcacc tccgacagcg aaagcgaagg tggtgcgggt ggtttcgatg
    21841 gcgctgaaga actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca
    21901 aagcgtcaat ttctggcgta caacgtcagt tccgcattgg ttataaccgt gcagcgcgta
    21961 ttatcgaaca gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg
    22021 aagtgctgtc cccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg
    22081 tcgcatccgg cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta
    22141 ttagcctgga atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata
    22201 atgatgaaaa aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt
    22261 tgggctgatg ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc
    22321 agcttcacac aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat
    22381 ctgtgggtga aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt
    22441 ctggtttctg acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca
    22501 acctggctga aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc
    22561 agcgactggc agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa
    22621 gccagcaatg gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat
    22681 cagtttagcg cggtggagca ggacgatcag cgcagcagtt atcaactgaa atcccagcaa
    22741 aatggggctg tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat
    22801 gatcaacgta agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac
    22861 ttttcaacct ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggtcagca
    22921 acatttgctg gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atttacattc
    22981 tatgatcctc tgggggccac cgggtaccgg caaaacaacc ctcgctgaag tgattgcccg
    23041 ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc tctggtgtga aagagattcg
    23101 cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt
    23161 tgacgaagtt caccgtttca acaaaagcca gcaggatgca tttctgccac atattgaaga
    23221 cggcaccatc acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc
    23281 actgctttcc cgtgcccgtg tttatctgtt gaaatccctg agtacagagg atattgagca
    23341 agtactaact caggcgatgg aagacaaaac ccgtggctat ggtggtcagg atattgttct
    23401 gccagatgaa acacgacgtg ccattgcaga actggtgaat ggcgacgcgc gccgggcgtt
    23461 aaatacgctg gaaatgatgg cggatatggc cgaagtcgat gatagcggta agcgggtcct
    23521 gaagcctgaa ttactgaccg aaatcgccgg tgaacgtagc gcccgctttg ataacaaagg
    23581 cgatcgcttt tacgatctga tttccgcact gcataagtcg gtacgtggta gcgcacccga
    23641 tgcggcgctg tactggtatg cgcgaattat taccgctggt ggcgatccgt tatatgtcgc
    23701 gcgtcgctgt ctggcgattg cgtctgaaga cgtcggtaat gccgatccac gggcgatgca
    23761 ggtggcaatt gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc
    23821 cattgctcag gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc
    23881 gtttaaagcc gcgctggccg atgctcgcga acgcccggat tatgacgtgc cggttcattt
    23941 gcgtaatgcg ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc
    24001 tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca
    24061 aacacgctat tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc
    24121 ctggctggct gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg
    24181 cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt
    24241 cgataagcac aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt
    24301 cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga
    24361 agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg
    24421 atcgaaatcc attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga
    24481 agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc
    24541 tgaaattcgc gatatcgcgc tgaccatccc taacctgcct gcagatgaag tgccggtagg
    24601 taaagacgaa aatgacaacg ttgaagtcag ccgctggggt accccgcgtg agtttgactt
    24661 tgaagttcgt gaccacgtga cgctgggtga aatgcactct ggcctcgact ttgcagctgc
    24721 agttaagctg actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg
    24781 cgcactgtcg cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta
    24841 tgttccgtac ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc
    24901 tggcgatctg ttccatactc gtccgctgga agaagaagca gacaccagta actatgcgct
    24961 gatcccaact gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga
    25021 tgatctgcca attaagatga ccgcccacac cccatgcttc cgttctgaag ccggttcata
    25081 tggtcgtgac acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca
    25141 gatcgtgcgc ccggaagact caatggcggc gctggaagag atgactggtc atgcagaaaa
    25201 agtcctgcag ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg
    25261 ctttggcgct tgcaaaactt acgacctgga agtatggatc ccggcacaga acacctaccg
    25321 tgagatctct tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg
    25381 ccgcagcaag tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc
    25441 tgttggtcgt acgctggttg cagtaatgga aaactatcag caagctgatg gtcgtattga
    25501 agtaccagaa gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat
    25561 ttttctgaat ctaaaaagcg cctacgggcg ctttttttgt ctccctttga taccgaacaa
    25621 taattactcc tcacttacac gtaatactac tttcgagtga aaatctacct atctctttga
    25681 ttttcaaatt attcgatgta tacaagccta tatagcgatc tgctatagaa ataattacac
    25741 aatacggttt gttactggaa tcaatcgtga gcaagcttga gtgagccatt atgaaaacga
    25801 aaatccctga tgcggtattg gctgctgagg tgagtcgccg tggtttggta aaaacgacag
    25861 cgatcggcgg cctggcaatg gccagcagcg cattaacatt accttttagt cggattgcgc
    25921 acgctgtcga tagcgccatt ccaacaaaat caggcgaaaa ggttatctgg agcgcctgta
    25981 cagttaactg tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt gaaatcaaat
    26041 atgtcgaaac ggacaatacc ggcgatgaca attacgacgg cctgcaccag gttcgcgcct
    26101 gcctgcgtgg gcgttccatg cgtcgccgtg tctacaatcc ggaccgcctg aaatatccga
    26161 tgaaacgagt cggggcgcgc ggtgaaggca aattcgagcg cattagctgg gaagaagcct
    26221 acgacatcat cgcgaccaat atgcagcgcc tgatcaaaga gtacggcaac gaatccatct
    26281 atctgaacta tggcaccggt acgctgggtg gcaccatgac ccgctcctgg ccgccgggaa
    26341 ataccctggt cgcgcggctg atgaactgct gcggcggcta tctgaaccat tacggcgact
    26401 actcctccgc gcaaattgcg gaaggtctga actataccta cggcggctgg gcagatggca
    26461 acagcccgtc ggatatcgaa aacagtaagc tggtagtgct gtttggtaat aaccctggcg
    26521 aaacgcgaat gagtggcggt ggggtgactt actatcttga acaggcacgc cagaaatcta
    26581 atgcccgcat gatcatcatc gatccgcgct ataccgacac cggtgccggg cgcgaagatg
    26641 agtggatccc tattcgtccg ggaacagatg ccgcactggt taacggtctg gcgtacgtca
    26701 tgatcactga aaacctggtg gatcaggcat tcctcgataa atattgcgtt ggctacgatg
    26761 agaaaaccct gccagccagt gcgccgaaaa atggccacta taaagcttat attctgggtg
    26821 aagggccaga tggcgtggct aaaacaccgg aatgggcctc gcaaatcacc ggtgttccgg
    26881 cagacaaaat catcaaactg gctcgtgaaa tcggcagtac caaaccggcg tttatcagcc
    26941 agggatgggg cccgcagcgt cacgctaacg gtgaaatcgc aacccgtgct atctcgatgc
    27001 tggcgattct gaccggtaac gttggtatta atggagggaa cagcggcgcg cgtgaaggtt
    27061 catacagttt accgtttgtc cgtatgccga ccttggaaaa cccgatccag accagcattt
    27121 cgatgtttat gtggaccgat gccattgaac gtggcccgga aatgacggcg ctacgtgatg
    27181 gtgtgcgcgg gaaagataag ctggatgtgc cgatcaaaat gatctggaac tatgccggta
    27241 actgcctgat taaccagcat tctgaaatca accgtaccca tgaaatcctt caggatgata
    27301 agaagtgcga gctgattgtg gttatcgact gccacatgac ctcatcggcg aaatatgctg
    27361 acatcctgct gcctgactgc accgcttccg aacagatgga ctttgcactg gatgcatcct
    27421 gcgggaatat gtcttacgtg attttcaacg atcaggtgat taaaccgcgc tttgaatgta
    27481 agaccatcta tgaaatgacc agcgaactgg caaaacgtct tggcgttgag caacagttta
    27541 ctgaaggccg tacccaggaa gagtggatgc ggcatctgta tgcccagtcg cgggaagcga
    27601 ttcctgaact gccaacgttt gaagagttcc gcaagcaggg gatctttaaa aagcgcgacc
    27661 cacaagggca tcacgttgct tataaagcct tccgtgaaga tccgcaggca aatccactga
    27721 ccacgccatc gggtaaaatt gagatttatt cgcaggcgct ggctgacatt gccgctacct
    27781 gggaattgcc agaaggcgat gtgatcgatc cactgccgat ctacacgccg ggctttgaaa
    27841 gttatcagga tccgctgaac aaacagtatc cgctgcagct tacaggtttc cactataaat
    27901 ctcgcgttca ctcaacttac ggcaacgttg atgtgctgaa agcggcttgc cgtcaggaaa
    27961 tgtggatcaa cccgcttgat gcccaaaaac gcggtatcca caacggcgat aaagtcagga
    28021 tctttaacga tcgtggtgag gttcatattg aggcgaaagt gacgccacga atgatgccgg
    28081 gtgtggtcgc actgggtgaa ggtgcctggt atgacccgga tgcaaaacgt gtcgataagg
    28141 gtggttgtat taacgtactg accactcaac gtccgtctcc tctcgctaag gggaatccgt
    28201 cacatacaaa ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac aacccagtat
    28261 ggatttttta ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct ggcttgtaaa
    28321 gactacaaag atttgatgcc agaagtcagc ttccgccgca tttatgaata tgctggtggc
    28381 gactggcagg aagataacgg tgtctggcac cagaacgtgt ttgcctacta tctgtcgatt
    28441 tcgtgcaacc actgcgaaga tccggcttgt actaaagtct gcccgagcgg tgcgatgcat
    28501 aaacgtgaag atggttttgt tgtggtcgat gaagatgtgt gcattggctg ccgctactgc
    28561 catatggctt gcccgtatgg cgcaccgcaa tataacgaaa cgaaaggcca tatgaccaaa
    28621 tgcgatggtt gttatgaccg tgttgcggag ggtaaaaagc cgatctgtgt tgaatcttgt
    28681 ccgctgcggg cgctggattt cggccctatc gacgagctgc gtaaaaaaca tggcgatctg
    28741 gcggccgttg cgccgttgcc gcgagctcac tttaccaaac cgaatattgt gatcaaaccc
    28801 aatgccaata gccgcccgac cggggatacc actggctatc tggcaaaccc gaaggaggtg
    28861 tgagatggga agtggatggc atgaatggcc gctgatgatc ttcacggtct tcgggcaatg
    28921 tgtggcgggt ggttttatcg ttctggcttt ggcgctgctt aaaggcgacc tgcgagcaga
    28981 agcccagcag cgtgttatcg cctgcatgtt tggcttatgg gtgctgatgg gcattggctt
    29041 tatcgcctct atgctccatc ttggttcacc gatgcgcgcc tttaactcgc tcaaccgggt
    29101 aggggcttca gcactcagta acgaaatcgc cagcggttcg atcttttttg ctgtaggcgg
    29161 catcggctgg ttgctggcaa tgctgaaaaa gctgtcacct gcattgcgta cgctatggct
    29221 ggtggtgacg atggttcttg gcgtcgtctt tgtctggatg atggtgcgtg tgtataacag
    29281 cattgatacc gttccgacct ggtacagcat ctggacgccg atgggcttct tcctgacgat
    29341 gttcatgggc ggcccgttac tgggttacct gttgctgagt ctggctggcg tcgatggctg
    29401 ggcgatgcgt ctgctgccag ccatttctgt actggcactg gtggtgagtg gcgtgatgtc
    29461 agtgatgcag ggcacagagc tggcaactat tcatagttct gtgcagcagg ccgcagcgct
    29521 ggtgccggac tatggtgcgc tgatgtcctg gcgaatcgtg cttttggccg ttgccctgtg
    29581 cttgtggatt gcaccacagc taaaaggtta tcagcctgcg gttccgctac tatccgtctc
    29641 attcattctg cttctggcag gggaattaat cggtcgcggc gtattctatg gtttgcatat
    29701 gaccgtgggg atggccgtcg caagctaatc agtaacaacc ggggtttcgg ccccggttct
    29761 cttttatttc tgcttcgtta acgtgtcata actggtcatc aaattacgat aatcaggaat
    29821 gtggttagag aacaacgtcg ccagtccttc aatatcatta cgccagtcac ggtgcagctc
    29881 gcaagccacg ccgaaccatg tcatcaattg cgcacctgct tgcgacatgc gatcccatgc
    29941 cgaatgccgg gtaatttcat taaaggtccc tgacgcgtca gtcacaacaa agacatcaaa
    30001 cccttcttca atggctgaaa gcgccgggaa tgccacgcaa acttcggtta ccacaccggc
    30061 aataattaac tgttttttac ctgtcgcttt gactgctttt acaaaatctt cgttatccca
    30121 ggcgttaata ttgccagggc gagcaatgta aggtgcatcg gggaattgtg ctttcagctc
    30181 tggaactaat gggccgttag gaccggtttc aaaactggtg gtaagaatag ttggtaagtt
    30241 gaaatactta gccaggtcac ccagcgccag cacgttattt ttaaacttat cgggttcgat
    30301 atcccgtaca agggaaagta aaccagcctg gtgatcaaca agcaaaacgg cagcatcatt
    30361 tttatcaaga cgaacatacg gtttggtcat cctcttctcc tttcgagagt cagtggtacg
    30421 tctgaaatcg gtcagacgcg atgcattgct ctgaaagcat agacgggaaa tatgagtttg
    30481 ctgtgaccat gaaatttttc gactgaacga agcgtatgaa atttgtgtta gatcaataaa
    30541 aacaatcaga tgagattaat catctcttca gcattagcgt tatgaatgag taatataaat
    30601 cgataatacc gccgctgcga tatgcgcgta ttttcaccca ttgacaatgt tttttgcggt
    30661 ggcatgatgc gcatgaaatt tgaacttcct cacggtttta attcatgtcc acgtataccc
    30721 ggcctgtcat gcttttgctg tctggcctgc ttttgttgac tctggcgatc gcggtgttaa
    30781 atacactcgt gccgctttgg ctcgcccagg aacacatgtc cacatggcag gtaggcgttg
    30841 tcagctcatc ctattttacc ggcaaccttg tcggtacatt gctgacaggg tatgtcatta
    30901 aacgcattgg ctttaaccgc agctattatc tggcctcctt catttttgcc gctggctgtg
    30961 ccggccttgg cctgatgatt ggtttctgga gctggttggc gtggcgtttt gtcgcgggcg
    31021 tcggctgtgc catgatttgg gtagtcgttg agagcgcgct gatgtgcagt gggacgtcac
    31081 gtaaccgtgg gcgtttgctt gctgcgtata tgatggttta ttacgtggga acatttttag
    31141 gccagttact ggtcagcaaa gtctcaaccg agctgatgag cgtattgccg tgggttacag
    31201 gtttgacgtt ggcagggatc ttaccgctgt tgtttacgcg tgtcctgaat cagcaggctg
    31261 aaaaccatga ttcgacgtca attacgtcaa tgctaaaact ccgtcaggcg cggcttggcg
    31321 tgaatggctg cattatctca ggaatcgtgc tgggatctct atatggcctg atgccgctgt
    31381 acctcaatca caaaggggtg agcaatgcca gcattggttt ctggatggcg gtactggtca
    31441 gtgcgggtat cctcggacaa tggccgattg gacgtctggc ggataagttt ggtcgactgc
    31501 tggtgttgcg tgttcaggtc tttgtcgtca ttctcggcag tatcgcgatg cttagccagg
    31561 cggcgatggc cccagcgtta ttcatcctcg gtgccgctgg ctttacgcta tatccggtgg
    31621 cgatggcctg ggcttgtgag aaagttgaac atcatcaact ggtggcgatg aaccaggcct
    31681 tactgttgag ctataccgtg ggaagtctgc ttggcccgtc atttaccgct atgctaatgc
    31741 agaatttctc cgataattta ttgtttatca tgatcgccag cgtatcgttt atctatttgc
    31801 tgatgctgct tcgcaacgcc ggtcatacgc cgaaacccgt tgctcacgtg taaatgaatt
    31861 caagcaaagt gtgaacttac tgtttcacac tctgcttttt tgtttcttct atctgacttg
    31921 cttttttcca aattttattc gtttaaaaat aaaatgtgca gcaggttata attttgcatt
    31981 tcgctatttc cgcacttctt atttgccgcg cataatccct cgttttacag atgccccttt
    32041 aattttggcg aaggatttgt ctatggctgg gaatgttcag gaaaaacagc tgcgatggta
    32101 caacattgcg ctgatgtctt ttatcactgt ctggggtttt ggcaacgttg ttaataacta
    32161 tgccaaccag gggctggtgg ttgttttttc atgggtgttt atctttgcgc tctatttcac
    32221 accttatgca ctaattgttg gtcagttagg ctcgaccttc aaagatggga agggcggggt
    32281 cagtacctgg attaaacaca cgatgggacc cggattggct tacctcgccg cgtggaccta
    32341 ctgggtggtg catattccct atctggcaca aaaaccccag gcaatactga ttgcgctcgg
    32401 ttgggcgatg aaaggcgacg gttcgctaat caaagaatat tcagtcgtag cgttacaggg
    32461 gttaacgctg gtgctgttta tcttctttat gtgggttgct tcacgcggta tgaaatcgct
    32521 gaaaatcgtc ggttctgtgg cagggattgc gatgtttgtt atgtcgctcc tgtatgtggc
    32581 gatggcggta accgcgcctg cgattactga agtgcatatt gcgaccacaa acattacctg
    32641 ggaaacgttc attcctcata tcgactttac ctacattacc actatttcaa tgctggtttt
    32701 cgcggttggc ggagctgaga agatttctcc ttacgttaat caaacgcgca acccaggaaa
    32761 agaatttcca aaagggatgt tatgcctggc ggtgatggtt gcggtttgtg ccattctggg
    32821 ctcactggcg atggggatga tgtttgactc gcgtaatatc ccggatgact taatgactaa
    32881 cggtcagtat tacgcctttc agaagctggg cgagtattac aacatgggta atactttaat
    32941 ggtgatttac gccattgcga ataccctggg acaagtagct gcgctggtat tctcgattga
    33001 tgccccgctt aaagtgctat taggcgatgc tgacagcaaa tatattccag ccagtttatg
    33061 tcgtaccaac gcttctggta cgcccgttaa tggctatttt ctgaccctgg tgctggtggc
    33121 gatcctgatt atgctgccga ctctcggcat cggtgatatg aacaatctct ataaatggct
    33181 gttgaatctt aactcggtag tgatgccgct gcgttattta tgggtatttg ttgcatttat
    33241 tgcagtcgtt cgcctggcgc agaaatatac accagagtat gtctttattc gtaataagcc
    33301 tctggcaatg accgtcggga tttggtgttt tgcctttact gcctttgcct gtttgacggg
    33361 gatcttcccg aaaatggaag ccttcactgc agagtggacc ttccagttgg cgctgaatgt
    33421 tgcaacgccg tttgtgctgg taggattagg actgatattc ccgctgctgg cgcgtaaagc
    33481 gaatagtaaa taattattgt ggtcggtgac gctgactctg gcagatctga cccgttttta
    33541 cataatcgag aaatgcgcgc aagccgcagg acatatactg gcggtttggg taatacattt
    33601 ggaagctgtg ctgtaagggc gttagaaata gagctctgaa ttatttatgc agcgcggaat
    33661 aaaaaagcgg caactcaata aagttgccgc tttacgggga aattagaaca ttaccttatg
    33721 accgtactgc tcaagaatac ctttcacgcg ttccatggtt tctttcttcg gtggtttaac
    33781 accatcgagt ttgtattctt cacccatcgc cacccatttg tgtttgccca gttcgtggta
    33841 ggggagaagt tcgattttct caacgttacc catatcacgg gtaaattcac cgaggcgatg
    33901 cgctgaatcg tcatcgtcag accagcctgg gacaacaacg tagcggatcc acaccttcac
    33961 atttttattc gccagatatt tggcgaactc cagcgtgcgg tggttggaaa ctccaaccag
    34021 attttggtgg atctcgtcgt tcatctgttt gagatcgagc attaccaggt cggttacttc
    34081 cagcagttca tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtgt ccagacaggt
    34141 atgaatgcct tcttttttgc aggcgcggaa ccagtcacga acaaactcag cttgcaggat
    34201 tgcttcaccg ccggatgcgg taacgccgcc gccggaagcg ttcataaagt ggcgataggt
    34261 caccacttcc ttcatcaaat cttcaacggt aacttcttta ccgccatgcg tgtcccaggt
    34321 gtcgcggtta tgacaataca ggcagcgcat caggcagccc tggaaaaagg tgataaagcg
    34381 aatacctggg ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac caataactga
    34441 cattgcggtg tttctccaga tgtggcccat ctgaggccgt gttggtgcgc agctcgaagg
    34501 ctacgtcgag tctgttttgg cagtcacctt aaagtataga tagctgacaa aaaaggctca
    34561 cgtgctaaaa aaggccccac tttcgtggag cctttattgt acgcttttta ctgtacgatt
    34621 tcagtcaaat ctaattacat agattgagtg aaggtacgag taataacgtc ctgctgctgt
    34681 tctttagtca gcgagttgaa acgtactgcg tagccagata cacggatggt cagctgcgga
    34741 tatttttccg gattttccat cgcgtcgagc agcatttcac ggttcatcac gttaacgttc
    34801 aggtgctgac caccttcgat ggatgcttcg tggtggaagt aaccatccat cagaccagcc
    34861 aggttggtct tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat agagaaggtg
    34921 taggagatac catctttagc gtaagcaaac ggcagtttag caacggaagt cagagacgct
    34981 acagcacctt tctggtcacg accgtgcatc gggttagcac ccggtccgaa cggcgcgcca
    35041 gcacgacgac cgtctggggt gttacccgtt ttcttaccat acacaacgtt agaagtgatg
    35101 gtcagaacag actgagtcgg gatagcgtca cggtaggtgt gcagtttctg aattttcttc
    35161 atgaaacgtt ctaccaggtc aacagccagg tcatctacac gcggatcgtt gttaccaaac
    35221 tgcgggtatt cgccttcgat ttcgaagtcg atagccagac cgtcttcgtc acgaatcggt
    35281 ttaactttcg catatttgat tgcagacagg gagtcagcag caacggacag accagcgata
    35341 ccacacgcca tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga ggcttcgtag
    35401 ctgtacttgt cgtgcatgta gtgaatgatg ttcagtgcag tgatgtactg tttagccagc
    35461 cagtccatga agtgatccat gcgctccatc acttcatcgt agttcaggag atcgcctttg
    35521 atcggttcag acttcggacc aacctgcatt ttcagttttt cgtcaacgcc gccgttgatt
    35581 gcgtacagca tggttttcgc caggtttgca cgcgcaccga agaactgcat ttgtttacca
    35641 acgatcatcg ggcttacgca gcaagcgata gcgtagtcat cgttgttgaa gtccggacgc
    35701 atcaggtcat cgttctcata ctgcagagaa gaggtgtcga tggacacttt agcggcgaat
    35761 ttcttgaagt tcagcggcag tttttcagac cacagaatgg tcatgttcgg ttccggagac
    35821 ggacccatgg tgtacagggt gttcaggaaa cggaagctgt ttttggtaac cagggtacga
    35881 ccgtcgaggc ccataccacc gatagattcg gttgcccaga tcgggtcgcc agagaacagt
    35941 tcatcgtact ccggagtacg caggaagcga accatacgca gtttcatgac caggtggtca
    36001 accatttcct gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg ttcgatgtac
    36061 acatccagga aggtggaggt acgaccgaag gacattgcag caccgttctg agacttaaca
    36121 gcagccaggt agccgaagta agtccactgg atagcttcct gagcgttggt agccggacca
    36181 gagatgtcgt agccgtattt cgcagccatt tctttcatct gacccagagc gcggtgctgt
    36241 tcagcgattt cttcgcgcag acggatagtc tgttccaggt ttacgccgtt ttccagatca
    36301 gcctgcagag aggtgaactg agcgtatttg tctttcatca ggtagtcgat accgtacagc
    36361 gcaacgcgac ggtagtcacc gatgatacgg ccacggccat aagcatctgg cagaccggtc
    36421 agaacaccgg atttacggca acgcaggatg tccggagtgt aaacgtcgaa cacgccctgg
    36481 ttgtgagttt tacggtattc agtgaagatt tttttgatca tcgggtccag ttcgcggttg
    36541 tacgctttgc aggaaccttc gatcattttg ataccaccga acgggataag agcacgtttc
    36601 agcggagctt cagtctgcag accaacaact ttttccaacg ctttgttgat gtagccagcg
    36661 tcgtgagagg tgatggtgga agcaacagcg gtgtcgaagt caactggcgc gtgagtgcgg
    36721 ttttccagtt taacgccttc cattactttg tcccacaggg tggtggtcgc ttcagtagcg
    36781 ccagccagga aggactcgtc accctcgtac ggagtgtagt ttttctgaat gaagtcacgg
    36841 acgtttactt cattctgcca gtcacctttg gtaaaacctt cccaggctgt ggctaacttt
    36901 tcattaagct cggacatgta acacctacct tcttaagtgg attttttatt tactgcgtac
    36961 ttcgacaacc attaatggtg atcgttttca cgcaggtaaa tgacccagta tgtcaaccca
    37021 accaacaaac caccgccgat aatattaccg atcgtaaccg gaatcaggtt atcagtgatg
    37081 aagttcatca cggtcaggtg agaaaaattt tccggtgcag aaccgacagc ggtccagaat
    37141 tccggagatg cgaagtcgcg gattacaata cccatcggga tcataaacat gtttgcgata
    37201 ctgtgctcaa aaccgctggc aacaaacatc gcgaccggca gcaccatgat aaacgctttg
    37261 tccatcaggc tgcggccaga ataactcatc catactgcca gacataccat caggtttgcc
    37321 aggataccaa ggcagacggc ctcaataaaa gtatggtgca ctttgtggtc ggcggtttgt
    37381 aggacgttta gtccccattg accatttgcg gtcatatact cgccggaaag ccacattaaa
    37441 agtacaaaca gcagtgcgcc gaccaggttg ccaaaataga catttagcca gtttttagcc
    37501 aactgacccc aggtgatgcg cccactcgcc ttagcaacaa caatcaacac tgtggaagta
    37561 aagagatcgg ctccgcagac aacacaaaga atcagtccca gagagaagca aatgccgcca
    37621 accagttttg ccatgccgaa gggcattgtg cctgtgccag tggttgctgt gatatagaag
    37681 acgaatgcga ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt cttaagcgga
    37741 tgtttcgttg ctttatagac acccgcctct tcggccactt tggccattgc agcaggaagt
    37801 aaaagatcaa aagggttgtc agctttcaca ctaactctct ctttattaag tcggcgacga
    37861 gatactaaca aagcattata gatgagaaat tgatatagat catatctcgc ctggcttata
    37921 ggcccgtaac tcgcatggtt tttatgcaaa tacggagtaa atatttgatt atccaaataa
    37981 aaataaattt taaaaattaa caaatgagtt gaattttttc cgcatcctcc gctaaaacag
    38041 ttaattaaaa gggagc
//
LOCUS       DAZFDC010000028        37799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFDC010000028 DAZFDC010000000
VERSION     DAZFDC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.028"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..913
                     /gene="ytfI"
                     /locus_tag="ACHJ48_001601"
     CDS             <1..913
                     /gene="ytfI"
                     /locus_tag="ACHJ48_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFO4707179.1"
                     /translation="FIGAVELFVKSSYTKTHSNNFFNNIHHAFKKKDWISNYDSLLTL
                     REFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDNGAEYTMTLRGSGFEYEEI
                     PITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQAIGDVSLGY
                     ALLDDIVSKLKRGKFELQGPGGGIKQCDGWYIYEKIIDDNFAIVIESLGFALKIYGGD
                     ERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSDITKEKICE
                     KYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(976..1530)
                     /gene="ytfJ"
                     /locus_tag="ACHJ48_001602"
     CDS             complement(976..1530)
                     /gene="ytfJ"
                     /locus_tag="ACHJ48_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO4707180.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1855..2061
                     /gene="ytfK"
                     /locus_tag="ACHJ48_001603"
     CDS             1855..2061
                     /gene="ytfK"
                     /locus_tag="ACHJ48_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO4707181.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2157..3500)
                     /gene="paeA"
                     /locus_tag="ACHJ48_001604"
     CDS             complement(2157..3500)
                     /gene="paeA"
                     /locus_tag="ACHJ48_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO4707182.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3823..4461)
                     /gene="msrA"
                     /locus_tag="ACHJ48_001605"
     CDS             complement(3823..4461)
                     /gene="msrA"
                     /locus_tag="ACHJ48_001605"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO4707183.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLHGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4667..6400
                     /gene="tamA"
                     /locus_tag="ACHJ48_001606"
     CDS             4667..6400
                     /gene="tamA"
                     /locus_tag="ACHJ48_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO4707184.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6397..10176
                     /gene="tamB"
                     /locus_tag="ACHJ48_001607"
     CDS             6397..10176
                     /gene="tamB"
                     /locus_tag="ACHJ48_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO4707185.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGILNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTEEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10179..10520
                     /gene="ytfP"
                     /locus_tag="ACHJ48_001608"
     CDS             10179..10520
                     /gene="ytfP"
                     /locus_tag="ACHJ48_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO4707186.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10732..10983
                     /gene="chpS"
                     /locus_tag="ACHJ48_001609"
     CDS             10732..10983
                     /gene="chpS"
                     /locus_tag="ACHJ48_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO4707187.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10977..11327
                     /gene="chpB"
                     /locus_tag="ACHJ48_001610"
     CDS             10977..11327
                     /gene="chpB"
                     /locus_tag="ACHJ48_001610"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO4707188.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11407..11937)
                     /gene="ppa"
                     /locus_tag="ACHJ48_001611"
     CDS             complement(11407..11937)
                     /gene="ppa"
                     /locus_tag="ACHJ48_001611"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO4707189.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12247..13203
                     /gene="ytfQ"
                     /locus_tag="ACHJ48_001612"
     CDS             12247..13203
                     /gene="ytfQ"
                     /locus_tag="ACHJ48_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO4707190.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13343..14845
                     /gene="ytfR"
                     /locus_tag="ACHJ48_001613"
     CDS             13343..14845
                     /gene="ytfR"
                     /locus_tag="ACHJ48_001613"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO4707191.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQARRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14859..15881
                     /gene="ytfT"
                     /locus_tag="ACHJ48_001614"
     CDS             14859..15881
                     /gene="ytfT"
                     /locus_tag="ACHJ48_001614"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO4707192.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIIT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15868..16863
                     /gene="yjfF"
                     /locus_tag="ACHJ48_001615"
     CDS             15868..16863
                     /gene="yjfF"
                     /locus_tag="ACHJ48_001615"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO4707193.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16896..17894)
                     /gene="fbp"
                     /locus_tag="ACHJ48_001616"
     CDS             complement(16896..17894)
                     /gene="fbp"
                     /locus_tag="ACHJ48_001616"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO4707194.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYSTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFICEFPDA"
     gene            18070..19443
                     /gene="mpl"
                     /locus_tag="ACHJ48_001617"
     CDS             18070..19443
                     /gene="mpl"
                     /locus_tag="ACHJ48_001617"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO4707195.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19599..20150)
                     /gene="yjgA"
                     /locus_tag="ACHJ48_001618"
     CDS             complement(19599..20150)
                     /gene="yjgA"
                     /locus_tag="ACHJ48_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO4707196.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20244..21596
                     /gene="pmbA"
                     /locus_tag="ACHJ48_001619"
     CDS             20244..21596
                     /gene="pmbA"
                     /locus_tag="ACHJ48_001619"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO4707197.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21780..22166
                     /gene="cybC"
                     /locus_tag="ACHJ48_001620"
     CDS             21780..22166
                     /gene="cybC"
                     /locus_tag="ACHJ48_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4707198.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(22211..22675)
                     /gene="nrdG"
                     /locus_tag="ACHJ48_001621"
     CDS             complement(22211..22675)
                     /gene="nrdG"
                     /locus_tag="ACHJ48_001621"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO4707199.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22865..25003)
                     /gene="nrdD"
                     /locus_tag="ACHJ48_001622"
     CDS             complement(22865..25003)
                     /gene="nrdD"
                     /locus_tag="ACHJ48_001622"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO4707200.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDISEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25397..27052)
                     /gene="treC"
                     /locus_tag="ACHJ48_001623"
     CDS             complement(25397..27052)
                     /gene="treC"
                     /locus_tag="ACHJ48_001623"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO4707201.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVALNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(27102..28523)
                     /gene="treB"
                     /locus_tag="ACHJ48_001624"
     CDS             complement(27102..28523)
                     /gene="treB"
                     /locus_tag="ACHJ48_001624"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO4707202.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28642..29589)
                     /gene="treR"
                     /locus_tag="ACHJ48_001625"
     CDS             complement(28642..29589)
                     /gene="treR"
                     /locus_tag="ACHJ48_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO4707203.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            29774..29827
                     /gene="mgtL"
                     /locus_tag="ACHJ48_001626"
     CDS             29774..29827
                     /gene="mgtL"
                     /locus_tag="ACHJ48_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO4707204.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29968..32664
                     /gene="mgtA"
                     /locus_tag="ACHJ48_001627"
     CDS             29968..32664
                     /gene="mgtA"
                     /locus_tag="ACHJ48_001627"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO4707205.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32870..33256)
                     /gene="ridA"
                     /locus_tag="ACHJ48_001628"
     CDS             complement(32870..33256)
                     /gene="ridA"
                     /locus_tag="ACHJ48_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO4707206.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33329..33790)
                     /gene="pyrI"
                     /locus_tag="ACHJ48_001629"
     CDS             complement(33329..33790)
                     /gene="pyrI"
                     /locus_tag="ACHJ48_001629"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO4707207.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33803..34738)
                     /gene="pyrB"
                     /locus_tag="ACHJ48_001630"
     CDS             complement(33803..34738)
                     /gene="pyrB"
                     /locus_tag="ACHJ48_001630"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO4707208.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34742..34876)
                     /gene="pyrL"
                     /locus_tag="ACHJ48_001631"
     CDS             complement(34742..34876)
                     /gene="pyrL"
                     /locus_tag="ACHJ48_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO4707209.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35157..35552)
                     /gene="yjgH"
                     /locus_tag="ACHJ48_001632"
     CDS             complement(35157..35552)
                     /gene="yjgH"
                     /locus_tag="ACHJ48_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO4707210.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35683..36396)
                     /gene="bdcA"
                     /locus_tag="ACHJ48_001633"
     CDS             complement(35683..36396)
                     /gene="bdcA"
                     /locus_tag="ACHJ48_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4707211.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36467..37060
                     /gene="bdcR"
                     /locus_tag="ACHJ48_001634"
     CDS             36467..37060
                     /gene="bdcR"
                     /locus_tag="ACHJ48_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO4707212.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARLHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            37205..37657
                     /gene="tabA"
                     /locus_tag="ACHJ48_001635"
     CDS             37205..37657
                     /gene="tabA"
                     /locus_tag="ACHJ48_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO4707213.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         9133 a         9367 c        10140 g         9159 t
ORIGIN      
        1 ttttattggt gcggtggagt tatttgtaaa gtcttcgtat acaaaaacac attcaaacaa
       61 tttttttaac aacatccatc atgcatttaa gaaaaaagat tggatttcga attatgatag
      121 cctgttaact ttgagggagt tctttcgttg cgcaactcag attgataaaa gtagttatca
      181 ggtgctttca tcaaaaaatg aaactgttaa cgctatggat aagtttctta tcagtttcag
      241 ccttaaagat aacggtgccg aatatacaat gacattgcga ggcagtggat ttgaatacga
      301 agaaatcccc ataacaataa atgagtataa ctcattcatg gatttcaaga accgtgaatt
      361 tccgttagaa caaaacagac gattgtatgc ctgggatatt ctgcagaaaa aacagtctga
      421 cataccgaaa aggattaaag gttatattca ccaggcgatt ggtgacgtgt ctttaggtta
      481 tgctttattg gatgatatag taagcaaatt aaaacggggt aaatttgaac tacaagggcc
      541 aggaggtgga attaaacagt gcgatggatg gtatatttac gaaaaaataa ttgatgataa
      601 ttttgccata gtcatagaat ctttgggctt tgcgcttaaa atctatggag gggatgaacg
      661 ttttcgtaat ggttcctctg ttgtcttgga ggatgaagat tactctctta tttataattt
      721 tctggttaat gcgggttgcc aacaagtcga attagccgaa caagtggatg cgatagtaag
      781 tgcgaatctg gccgcagata gtgatattac caaagaaaag atttgcgaga aatataaatc
      841 gaccattgag gcgtttaaaa aagagcaact agcgttacca gtattagttc ggcgtaagaa
      901 ctcagagact taattcccct atcccataga taacgatagg ggaaaattat ttttgcagaa
      961 tttatgttat tgccattatt tgagcaactt ttgcagcagg tccatcactt gctgcacctc
     1021 ttcctgagtg agcgccccgt ctttggccca ttgcacgcga ccgtctttat ccagcaccac
     1081 tacagcggaa ctttcttcat ccagctgcca ggcaccgcgc gcgacgccat tgctatcgac
     1141 aataaactgc gaccagggat aaagcttttt attactctcc agactgctgc gcacaaacat
     1201 gccggaaccc ggaatcgcgt cgtcggtgtt aacaatggtg gtggtctggt aacgatcgtg
     1261 cggtaacttc gctgatttaa tcgcttcaat cagcgtcgcg tttttctctt ttgcagaggt
     1321 gcgaccagca atatgttgca gtactcgcac ttttcccact aactgcgcgc tgttccaggt
     1381 tttgtagcta aactgatctt tatcaagcac caactcgccc cgatcggtaa tgccaatcgg
     1441 cggcactcgc tgaccggttt cgaactgatg tgcggaagcc atcatcggca acagcaggca
     1501 ggtgagtgcc agaatcttgc gtagggtcat ggtgtttcct tcttatgata tgcaggtgat
     1561 ccgaccactt gggtctgagt tttaatcata tgtgctattt atcgaatttc ccgcaagtgt
     1621 gatgccagtt tgcggtcaag cgcacaaatc atatgaaaaa tgaatgctta tactgaagac
     1681 cgcgcttcgg taaaaagata attctgaata attgtaacct ttaggtaaaa aaagttatac
     1741 gcggtggaaa cattgcccgg atagtctata gtcactaagc attaaaattt gcgcctcata
     1801 atagttgggc cgattgtggc accgcacagg cgtaatactc agcaggagat aacaatgaaa
     1861 attttccaac ggtacaaccc acttcaggtg gcgaagtacg taaagatcct gttccgtgga
     1921 cgattataca tcaaggacgt tggcgctttt gaattcgata agggtaagat tcttatccca
     1981 aaagtgaagg ataagctgca tttgtcagtg atgtccgaag ttaaccgtca ggttatgcgt
     2041 ctgcaaacag agatggctta accaaagtgc tatgcagtaa aaagtgctat gcagtaaaaa
     2101 gtgctatgca gtaataagac ggctcctgat tcaggagccg ttgatgttta tgggggttac
     2161 gcgacgcttt cttctttatc tttagcgtca ggcagttttg gcgaaagggc tgtggccttg
     2221 ctgtcgatcc gggtcaccag cagctggtcg atgcggtagt tatcgatatc caccacttca
     2281 aatttgtagc cggcgaattt caccgaatcg gtgcgtttcg ggatcttacg cagcataaac
     2341 atcataaagc cgccgatggt ttcgtagttg cccgactgcg ggaactcgtc aatatccagc
     2401 acgcgcatga cgtcgtcaat cggagtgccg ccgtcaatca gccatgagtt ctcatcacgg
     2461 gcgacaatct gctcttccag tccctgaccg accagatcgc ccatcagcgt ggtcatcacg
     2521 tcgttgaggg tgatgatccc cactaccagc gcgtactcgt tcatgatcac cgcgaagtct
     2581 tctcctgcgg ttttaaaact ttccaacgcc tctgaaaggg ttaacgtatc cggcacaatc
     2641 agcgtgttgc gaatttgtac gccgctgttc agtgccaggc tttggttagc cagcacgcgg
     2701 ttcagcaggt ctttagaatc gacatagccg atgatgtggt caatatcttc attacagacg
     2761 aggaacttag agtgcggatg ttccgccacc ttatttttca gactttgctc atcttcgtgg
     2821 agatcaaacc aaatcacgtt ttcacgcggt gtcattgaag acggaacggt acgggattcc
     2881 agctcaaaga cgttttcaat cagctcgtgt tcctgtttac gtaacacgcc cgccagcgca
     2941 ccggcttcca ctaccgcgta gatgtcatca gaagtgatgt catctttacg taccattggc
     3001 agtttgaaaa tacggaagat tatgttcgcc aggccgttga agaaccacac cagcggggtg
     3061 caaacgtaca ggcagaagcg catcgggttg atgatacgca aagccacagc ttctggcgca
     3121 atcataccga tgcgtttcgg ggttaaatcc gcaaacagga taaacatgcc agtcactaac
     3181 gagaaagaga gaataaagct cagttgctca gagagctctg ccgacatata gcgggagaac
     3241 aggctgtgaa aagctggaga aaatgccgca tcaccgacga taccgccgag aatcgccact
     3301 gcgttcagac cgatttggac cacggtaaag aacatgccgg gattttcctg catattcaga
     3361 acgcgttggg catttatatt gccttcatca gccagcagtt taagtttgat tttgcgtgag
     3421 gcggcaagtg agatctcaga catcgagaag aacgcactta cagcgatcaa gcagagtatg
     3481 actaaaatac tgtttaacat atcttatccg acctttcagg ccagatcctc ggaaggggaa
     3541 gttgattaat ttgtgtgaaa tacacattga aagccgattc gaagaaagtg aaccggcatt
     3601 ttcagtggct agtatagcgt aaggtactgt aaagccgcca gagggttaaa attcagacag
     3661 ctgaaaaatg caaaactgcc tgatgcgcta tgccgatcag gcctacgcag tccttgcaat
     3721 atattgaatt tggacgattt tgtaggcctg gcaaggcgtt tacgccgcat ccggcataaa
     3781 caaaacgcac tttatcaaca atctacattt gtacccgtaa cgctatgctt ccggtggcag
     3841 acagacgcca attccgccaa ttccacagta accgtacggg tttttatgca gatattgctg
     3901 gtggtcatct tcggcataat aaaacggtgt ggcgttagcg atttccgtgg tgatgtgacg
     3961 atcgtcatcg gcggcaagca tcgccgcctg aaaacgttcc agactggcgc gagctgcggc
     4021 atcctgttct ggggtcagcg gataaatcgc tgaacgatac tgcgtgccgt ggtcattgcc
     4081 ctgacgcatt ccctgggcgg gatcgtgatt ctcccaaaat acctgtagca actgctcata
     4141 gctgatgacg gaaggatcgt aaacaatgcg taccgcttcg gcatgaccag tatcaccgga
     4201 gcacacttcc cgataagtcg gatttggcgt atagccgccg gtataacctg cggcggtgct
     4261 gtaaacgccg tgtaactgcc agaacagacg ctccacaccc cagaaacaac ccatcgcaaa
     4321 aatggcaatc tccattccgt caggtacatt ggtcattgag tgaccgttaa ccgcatgcag
     4381 cgtggctacg ggcatcgggg tgttacgtcc aggcagggca tcggcggggg aaaccagatg
     4441 ctttttatca aataaactca tggtgtcgct ctcccgaaaa tcggtcattg gggttaaggt
     4501 tgtaacaaga ggcgtatttg cacacaataa ccaccgtgaa taggtctaaa gtaaaacata
     4561 agaaatattt ggggtttagt ctgcttttta atccatatta ctggattttt gttaagccgt
     4621 ttaacggcgt tccaggggca ggaaaaaagg atattcagga gaaaatgtgc gctatatccg
     4681 acagttatgc tgtgtaagct tactctgctt aagcggatct gccgtcgccg cgaacgtccg
     4741 tctacaggtc gaggggttat cgggacagct ggaaaagaac gttcgtgcgc agctttctac
     4801 gattgaaagt gatgaagtga cgccagaccg tcgctttcgc gcacgcgtcg atgatgctat
     4861 ccgcgaaggt ctgaaagcgc tgggttatta ccagccgacc attgaatttg atctccgtcc
     4921 accgccaaag aaagggcggc aggtattgat cgccaaagtc acgccaggcg tgccggtgtt
     4981 aattggcggc accgatgtgg tattgcgcgg gggcgcgcgg accgataaag actatttgaa
     5041 attgctcgat actcgcccgg ctattggcac ggtactgaac cagggcgatt atgaaaattt
     5101 caaaaagtcc ttaaccagca ttgcgttgcg taaaggttat ttcgatagcg aatttaccaa
     5161 agcgcagctg ggcattgcgc tcggcctgca taaagccttc tgggatattg attataacag
     5221 tggcgaacgt taccgctttg ggcatgtgac ctttgaagga tcacaaatcc gcgatgaata
     5281 cctgcaaaat ctggtgccgt ttaaagaggg cgatgagtac gaatcgaaag atctggcaga
     5341 actgaaccgc cgactttctg ctaccggctg gtttaactcg gtggtggtgg ctccacaatt
     5401 tgataaagcg cgcgaaacga aagtattacc attgacgggc gtggtttcgc cgcgaacaga
     5461 aaacactatc gaaaccgggg tcggttactc tacggacgtg ggaccgcgcg tgaaagcgac
     5521 gtggaaaaaa ccgtggatga actcatacgg tcacagtctg accaccagta ccagtatttc
     5581 cgcgccggaa cagaccctcg acttcagcta taaaatgccg ctgctgaaga atccactgga
     5641 acaatattat ttggtgcagg gcggttttaa gcgcactgac ctgaacgata ccgagtctga
     5701 ctccactacg ctggtggctt ctcgctactg ggatctctcc agcggctggc agcgtgccat
     5761 taacctgcgc tggagtctcg accactttac tcagggtgaa attaccaaca ccacgatgct
     5821 gttttatcct ggggtgatga ttagccgcac gcgttctcgt ggtggcctga tgccaacctg
     5881 gggcgactcg caacgctact ctatcgacta ctccaacacg gcctggggtt cagatgtcga
     5941 tttctccgtt ttccaggcgc agaacgtctg gatccgcaca ctgtacgatc gccatcgttt
     6001 tgtgacacgc ggcacgctgg gctggattga aaccggtgat ttcgacaaag taccgccgga
     6061 tctgcgtttc ttcgccgggg gcgatcgcag tattcgcggc tacaaataca aatctatcgc
     6121 cccgaaatac gctaacggtg acctgaaagg ggcctcgaag ttgataaccg gatcgctgga
     6181 ataccagtac aacgtgaccg gaaaatggtg gggcgcggtg tttgtcgata gtggcgaagc
     6241 ggtaagcgat attcgccgca gcgactttaa aaccggtacc ggggtcggcg tacgctggga
     6301 atcgccggtc gggccaatca aactcgattt tgccgtaccg gtcgcggata aagacgaaca
     6361 cgggttacag ttttacatcg gtctggggcc agaattatga gtttatggaa aaaaatcagc
     6421 ctcggcgtgg ttatcgttat cttactgttg ctgggatcgg tggcgtttct ggtgggcacc
     6481 accagcggcc tgcatctggt atttaaagcg gcggatcgct gggtgccagg actggatatt
     6541 ggcaaggtca ccggcggctg gcgcgatctc accttgtctg acgttcgtta tgagcagcca
     6601 ggcgtggcgg taaaagcggg taatctgcat ctggcggtcg gtcttgagtg cctgtggaac
     6661 agcagtgttt gtattaatga cttggcgctg aaagacattc aggtcaacat cgacagtaaa
     6721 aaaatgcctc cttctgaaca ggttgaagaa gaggaagata gcggtccgct ggatctctcc
     6781 acgccgtatc ccatcaccct gacacgggtg gcactggaca acgtcaacat caagattgat
     6841 gacaccacgg tatcggtgat ggacttcacc tccggcctga actggcagga gaaaaccctg
     6901 accctgaaac cgacgtcgct gaaaggcctg ctgattgctc tgccgaaagt ggcggaagtg
     6961 gcgcaggaag aagtggtcga accgaaaatt gaaaatccgc agccggagga aaagcctctc
     7021 ggcgaaacac tcaaagatct cttttctcgc ccggtattgc cggaaatgac cgacgtgcat
     7081 ctgccactca acctgaacat tgaagagttt aagggagagc aactgcgcgt gacgggcgat
     7141 acggacatta ccgtgcgcac catgctgctg aaagtgagca gcattgacgg caatactaaa
     7201 ctggacgccc tggatatcga ttccaatcaa gggatcctca acgccagcgg cacggcgcag
     7261 ctgtcagaca actggccggt ggatatcacc ctcaacagca cactgaacgt ggagccgttg
     7321 aaaggtgaaa aggtgaagct gaaagtgggc ggcgcgctgc gcgaacagct ggagattggc
     7381 gttaatcttt ccggtccggt ggatatggat ttacgcgccc agacgcgact ggcggaagcc
     7441 ggattgccgc tcaacgtgga agtgaacagc aaacagattt actggccgtt cactggtgag
     7501 aagcagtatc aggcggatga tctgaaactg aaacttaccg gcaaaatgac cgattacacg
     7561 ctctctatgc gtacggcagt gaagggactg gagatcccgc cagccaccat tacccttgat
     7621 gccaaaggta atgaacagca ggtcaatctc gacaaactca ccgtcgcggc gctggaaggg
     7681 aaaactgaac tcaaggcgtt gcttgactgg cagcaggcca ttagttggcg cggtgagcta
     7741 acgcttaacg gcattaacac cgccaaagag atcccagagt ggccgtcgaa actcaatggc
     7801 ttgattaaaa cccgcggtag cctgtacggc ggcacctggc agatggaggt gccagaactg
     7861 aagcttaccg gtaacgtcaa acagaacaaa gtgaacgttg acggcacgct gaaaggcaac
     7921 agttatatgc agtggatgat cccagggctt catctggaac tggggccaaa cagtgccgaa
     7981 gtgaaaggcg agctgggggt aaaagatctc aatcttgatg ccaccatcaa cgcgccgggg
     8041 ctggataacg cgctgccggg gcttggcggt acagcgaaag ggctggtgaa agttcgcggc
     8101 acggtggaag cgccacaact actggcggac atcaccgcgc gcggcctgcg ctggcaggag
     8161 ctaaccgtgg cgcaggttcg cgtggaaggc gatatcaaat ccaccgatca gattgcgggt
     8221 aaactcgacg tgcgcgttga gcaaatttca cagccggatg taaatatcaa cctcgtcacc
     8281 ctgaatgcca aaggcagcga aaaacagcac gagttacagt tgcggattca gggcgagccg
     8341 gtttccgggc agcttaatct ggcaggaagt tttgatcgca aagaagaacg ctggaaggga
     8401 actcttagca atacccgctt ccagacgctg gtcggcccgt ggtcgctgac ccgcgatatt
     8461 gcgctggatt accgcaataa ggagcaaaaa atcagcatcg ggccacactg ctggcttaac
     8521 ccgaatgcgg aactgtgcgt gccgcaaact atcgatgcgg gggccgaagg gcgtgcggtg
     8581 gtgaatctca accgcttcga ccttgccatg ctgaaaccgt ttatgccaga aaccactcag
     8641 gccagcggta tcttcacggg taaagcggac gttgcctggg acaccaccga agaggggctg
     8701 ccgcagggca gtatcaccct ttcggggcgt aacgtgcagg taacgcaaac cgtcaacgat
     8761 gcggcgctgc ccgtggcgtt tcagacactg aatctgacgg cggaattgcg taacaaccgt
     8821 gccgaattgg gctggaccat ccgcctgacc aataacggcc agtttgatgg acaggtgcag
     8881 gtgaccgatc cgcaaggccg ccgtaatctt ggtggcaacg tcaatatccg taacttcaac
     8941 cttgcgatga taaaccccat ctttacccgt ggggaaaaag cagcggggat ggtgagtgcc
     9001 aacttgcgtc tgggtggtga tgtgcaaagc ccgcagttgt ttggtcagct tcaggttacg
     9061 ggtgtggata tcgatggcaa ctttatgccg tttgatatgc aaccgagcca gcttgcggtc
     9121 aactttaacg gtatgcgctc gacgcttgcc ggtacagtac gaacccagca gggtgaaatc
     9181 tacctgaacg gtgatgccga ctggagccaa attgaaaact ggcgggcgcg ggtaacggcg
     9241 aaaggcagta aagtgcggat caccgtgccg ccgatggtac gaatggatgt atcgccagat
     9301 gttgtattcg aggctacacc aaacctgttt accctcgatg gtcgcgtgga tgtcccttgg
     9361 gcgcgcatcg tggtgcacga tctgccggaa agcgcagtag gcgtctccag cgatgtggtg
     9421 atgcttaacg ataacctgca accggaagag ccgaaaacgg cgtcgattcc gattaacagt
     9481 aacctgattg tccacgttgg caacaatgtg cgcattgacg cctttggcct gaaagcgcgg
     9541 ctgacgggcg atcttaacgt cgttcaggac aaacaagggc tgggcctgaa cgggcagatc
     9601 aacatccctg aagggcgctt ccatgcctat ggtcaggatc tgattgtgcg taaaggtgag
     9661 ttactgttct ctggcccgcc ggatcaaccg tatcttaata ttgaagctat tcgtaacccg
     9721 gatgctacag aagacgacgt aatcgccgga gttcgcgtca ctggtctggc ggacgaaccg
     9781 aaagcggaga tcttctctga cccggcgatg tcgcaacaag ctgccttgtc ttatttgcta
     9841 cgtggacaag ggctggagag cgatcagagc gacagtgcgg caatgacctc gatgctgatt
     9901 ggtctggggg ttgcgcaaag tggccagatt gtaggtaaaa tcggcgagac gtttggcgta
     9961 agcaatttag cgctcgacac ccagggagta ggcgactcct cccaggtagt ggtcagcggc
    10021 tatgtattgc caggtctgca agtgaaatac ggcgtgggta tatttgactc tatagcaaca
    10081 ctcacgttac gttatcgcct gatgcctaag ctatatctgg aagccgtgtc tggtgtagac
    10141 caggcactgg atttgctcta tcagttcgag ttttagcaat gcgaatattt gtctacggca
    10201 gtttacgcca caaacaaggc aacagtcact ggatgaccaa tgcccagtta ctgggcgatt
    10261 tcagtatcga taactaccag ttgtatagcc tgggccacta tccaggcgca gttccgggga
    10321 acggaacggt acacggtgaa gtttatcgta ttgacaacgc cacgctggcc gaacttgatg
    10381 ccttgcgcac caggggcggt gaatacgcgc gccagttgat tcagacgccg tacgggagtg
    10441 catggatgta cgtttatcaa cgacccgtcg atggattaaa gctaattgaa agcggcgact
    10501 ggttagacag ggataagtaa ccctatgcat acgccacctt cgggtggcgt tgttttttgc
    10561 gagacgactc gcattctgtt ttgtaattcc ctcacctttt gcttttctct ccgagccgct
    10621 ttccatatct attaacgcat aaaaaactct gctggcattc acaaatgcgc aggggtaaaa
    10681 cgtttcctgt agcaccgtga gttatacttt gtataactta aggaggtgca gatgcgtatt
    10741 accataaaaa gatgggggaa cagtgcaggt atggtcattc ccaatatcgt aatgaaagaa
    10801 cttaacttac agccggggca gagcgtggaa gtgcaggtga gcaacaacca actgattctg
    10861 acacccatct ccaggcgcta ctcgcttgat gaactgctgg cacagtgtga catgaacgcc
    10921 gcggaactta gcgagcagga tgtctggggt aaatccaccc ctgcgggtga cgaaatatgg
    10981 taaagaaaag tgaatttgaa cggggagaca ttgtgctggt tggctttgat ccagcaagcg
    11041 gccatgaaca gcaaggtgct ggtcgacctg cgcttgtgct ctccgttcaa gcctttaatc
    11101 aactgggaat gacgctggtg gcccccatta cgcagggtgg aaattttgcc cgttatgccg
    11161 gatttagcgt tcctttacat tgcgaagaag gcgatgtgca cggcgtggtg ctggttaatc
    11221 aggtgcggat gatggatcta cgcgcccggc tggcaaagcg tattggtctg gctgcggatg
    11281 aggtggtgga agaggcgtta ttacgcttgc aggcggtggt ggaataaggt gtgtttattt
    11341 atcgcgggca taaaaaaacc cttactaacc gaagcccggc gttcagggtt attacgccag
    11401 aagaacttat ttattctttg cgcgctcgaa ggaggcaacg atttcagctt tagcggcttc
    11461 tgcgttttcc caaccttcaa ctttcaccca cttgcctttt tcgaggtctt tgtagtgctc
    11521 gaagaagtga gcgatttgcg ctttcagcag ttcaggcaga tcgttaacgt ctttaatgtg
    11581 atcgtattct ttgctcagct tgctgtgcgg aaccgcaacc agtttcgcat cttcaccggc
    11641 ttcgtcggtc attttcagaa cgccaaccgg acggcaacgg atcacagaac ccggctgcag
    11701 cgggtacgga gttgggacca gtacgtcaac tgggtcaccg tccagagaca gggtgtggtt
    11761 gatgtaaccg tagttgcacg gatagaacat cgcggtggac atgaagcggt caacgaacag
    11821 tgcgccgctc tctttgtcga tttcgtattt gatcggatct gcgttagccg ggatctcaat
    11881 aacaacgtag atgtcttccg gcagatcttt acccgcaggg acgttgagta agctcatgtc
    11941 tgtttccttt aaaaatatgt ggcaaacaag tgccgagtat tatagccaac tcgcgccgaa
    12001 tgtcttcgct tgttttcgtc ttcattttcc tttttcgcca gttttaagac ggtatgcaga
    12061 gcaggaaaat ccataaccgt agccgcattt ttcatagtga gatgaaagcg attacaaact
    12121 tgtgattaac gttttattta cttttttgaa gtgtgatgta acgcaatccg ttacataacg
    12181 aattgtctat agttttttcg cgaacatctt ttcaccaata ataattactc cgacgaggac
    12241 aaccctatgt ggaaacgctt acttgtagtc tctgcagtct cggcagccat gtcgtctatg
    12301 gcgttggccg ctccattaac cgtaggattt tcgcaggtcg gatcggaatc cggctggcgc
    12361 gccgcagaaa ccaatgtggc gaaaagtgag gccgaaaaac gcggaattac gctgaaaatt
    12421 gccgatggtc agcaaaagca ggaaaaccag attaaagcgg tacgttcctt cgttgcacaa
    12481 ggggtggatg cgatctttat cgctccggtg gtcgcgacag gttgggaacc ggtattaaaa
    12541 gaggcgaaag atgccgaaat cccggtcttc ttgctcgacc gttccatcga tgtgaaagac
    12601 aaatctctct atatgaccac cgttactgcc gacaacatcc tcgaaggtaa gttgatcggt
    12661 gactggctgg taaaagaagt gaatggcaaa ccatgcaacg tggtggagct gcagggcacc
    12721 gttggggcca gcgtcgccat tgaccgtaag aaaggctttg ccgaagccat taagaatgcg
    12781 ccaaatatca aaatcatccg ctcgcagtca ggtgacttca cccgcagtaa aggcaaagaa
    12841 gtcatggaga gctttatcaa agcggaaaac aacggcaaaa acatctgcat ggtttacgcc
    12901 cataacgacg acatggtgat tggtgcaatt caggcaatta aagaagcggg cctgaaaccg
    12961 ggcaaagata tcctcacggg ttccattgac ggtgtaccgg acatctacaa agcgatgatg
    13021 gatggcgaag cgaacgccag tgttgaactg acgccgaaca tggcgggacc cgctttcgat
    13081 gcgctggaga aatacaaaaa agacggcacc atgcctgaaa agctgacgtt aaccaaatcc
    13141 accctttacc tgcctgatac cgcaaaagaa gagttagaga agaagaaaaa tatggggtat
    13201 tgagggttgc tatgcctgat gccgattcgt aggccggata aggcgctcgc gccgcatccg
    13261 gcgatggtgc actgaagcct gatgcgacgc ttaccgcgtc ttatcatgcc tactgggagc
    13321 acgctttaca ccgggggaaa ccatgacgac cgaccaacac caggagatcc tccgcaccga
    13381 aggattaagt aaatttttcc ccggcgtcaa agcgttagac aacgttgatt tcagcctgcg
    13441 ccgtggcgaa atcatggcgc tgctcggtga aaacggggcg ggaaaatcaa cgctaatcaa
    13501 agcattaact ggtgtatacc acgctgatcg cggcaccatc tggctggaag gccaggctat
    13561 ctcaccgaaa aataccgccc acgcgcaaca actcggcatt ggcaccgtct atcaggaagt
    13621 caacctgcta cccaatatgt cggtcgctga taatctattt ataggccgcg aacccaaacg
    13681 cttcggcctt ctacgccgca aagagatgga aaagcgcgcc accgaactga tggcatctta
    13741 cggtttctcc ctcgacgtgc gcgaaccgct caaccgcttt tcagtcgcga tgcagcaaat
    13801 cgtcgctatt tgccgggcta tcgatctctc tgccaaagtg ctgatcctcg atgaacccac
    13861 cgccagtctc gacacccagg aagtggagtt actgtttgac ctgatgcgtc agttgcgcga
    13921 tcgcggcgtc agcctgatct ttgtcactca ctttctcgat caggtctatc aggtcagcga
    13981 tcggatcacc gtcttacgca acggcagttt cgtaggctgt cgggaaacgt gcgagctacc
    14041 gcagatcgaa ctggtaaaaa tgatgctggg gcgcgagctg gatacccacg cgctacagcg
    14101 tgccgggcga acattgttga gcgacaaacc cgttgccgcg ttcaaaaatt acggcaaaaa
    14161 aggaacgatc gcaccgtttg atctcgaagt acgccccggc gagatcgtcg gtctggctgg
    14221 attgctggga tcaggacgta ccgaaaccgc cgaagtgatc ttcggtatca aacctgctga
    14281 cagcggcacg gcgttgatca aaggcaaacc gcaaaacctg cgatcgccac atcaggcttc
    14341 ggtacttggc attggcttct gcccggaaga caggaaaacc gatggcatca tcgctgccgc
    14401 ctcggtgcgg gaaaatatca tcctcgctct ccaggcccgg cgcggctggc tacgtcccat
    14461 ttcccgcaaa gaacagcaag agattgccga acgctttatc cgccagcttg gcattcgcac
    14521 accttcaact gaacaaccga ttgaatttct ctccggcggc aatcagcaaa aagtgttgct
    14581 ttcacgttgg ctactgaccc gaccgcaatt tctgatcctc gatgagccaa cccgcggcat
    14641 tgatgttggt gcccacgccg agatcatccg cctgattgaa acgctatgcg ccgatggtct
    14701 ggcgctgctg gtgatctcct ccgaactgga agaactggtg ggctatgccg accgggtgat
    14761 catcatgcgc gatcgcaaac aggtggcgga gatcccgctg gcagagcttt ccgttccggc
    14821 gatcatgaat gccattgcgg cgtaaggaga acagtgtgat gcctcaatct ctcccggaca
    14881 ctacgccgcc aaaaaggcgc tttcgctggc caacgggaat gccgcagctg gcggcactgt
    14941 tgctggtgct gctggtcgat agcctggtgg ccccgcattt ctggcaggtg gtgcttcagg
    15001 atgggcgttt gttcggtagc cctatcgaca ttcttaatcg cgccgccccc gttgcgctac
    15061 tggcgattgg tatgacgctg gtgatcgcaa caggtgggat tgatctctcc gtaggggcgg
    15121 tgatggctat cgccggagcc acaacggctg cgatgacggt cgcgggattc agcctgccga
    15181 ttgttttgtt aagcgccctg ggcaccggca tcctggcggg attgtggaac ggcatactgg
    15241 tagcgatcct caaaattcag ccgtttgtcg ccactctgat cctgatggtc gccgggcgcg
    15301 gcgtggcgca actgatcacc tccggacaga tcatcacgtt taactcgccg gatctctcat
    15361 ggttcggcag tggatcgctg ttgttcctgc caacgccggt cattatcgcg gtgctgacgc
    15421 ttatcctgtt ctggctgttg acccgcaaaa cggcgctggg gatgtttatc gaagccgttg
    15481 gtatcaacat tcgggcggca aaaaatgccg gggtaaacac gcggatcatc gtcatgctta
    15541 cctacgtgtt gagcgggctg tgtgcggcga ttgcgggcat tatcgtggcg gcggatattc
    15601 gcggtgccga tgccaacaac gccggattat ggctggagct ggacgccatt ctcgcggtgg
    15661 tgattggcgg tggttcgctg atgggcggac gttttaacct actgctttcg gtggtggggg
    15721 cgctgattat tcaggggatg aacaccggaa ttttgctttc gggctttccg ccagagatga
    15781 accaggtggt gaaagcggtg gtggtgcttt gcgtgctgat tgtccagtcg caacgcttta
    15841 tcagtctgat taaaggagtg cgtagccgtg ataaaacgta atttgccact gatgatcacc
    15901 atcggcgtct ttgtgctggg ttatctctac tgcctgaccc agtttcccgg ttttgcttcc
    15961 acaagagtga tctgcaatat cctgaccgat aatgcctttc ttgggataat cgccgttggc
    16021 atgacctttg tgatcctctc cggtgggatc gatctctccg ttggttcggt gatcgccttt
    16081 accggcgtgt ttctggcaaa agtgattggc gatttcggcc tctcgccgtt gctggcattt
    16141 ccgctggtgc tggtaatggg ctgtgccttt ggcgcattta tggggctttt gatcgacgcc
    16201 ctgaagatcc cggcatttat cattactttg gcaggaatgt tctttttgcg cggcgtcagc
    16261 tacctcgttt cggaagagtc gatcccgata aaccatcctg tttatgacac gctctcaagc
    16321 cttgcatgga aaattcctgg cggcggtcgc ttaagtgcga tggggctgct gatgctggcg
    16381 gtggtggtta tcggcatatt cctcgcgcat cgtacccgct ttggtaatca ggtgtacgcc
    16441 attggcggca atgcgacgtc ggcaaacctg atggggattt ccactcgcag cacaactatt
    16501 cgcatttata tgctctctac cggactggcg acactggcgg ggattgtctt ctcgatttat
    16561 acccaggccg gatacgcgct ggcgggcgta ggtgtggaac tggacgctat cgcctcagtg
    16621 gtgattggcg gtacgctttt gagcggtggc gttggaacgg tattagggac gctttttggc
    16681 gtggcgattc agggactgat tcagacttac ataaactttg atggtacgct gagctcatgg
    16741 tggacgaaaa tcgccatcgg tattttgttg tttattttta tagcattaca gcgtggatta
    16801 acggtgctgt gggagaatcg tcagagttcg cctgtgacaa gagtcaacat tgcgcagcaa
    16861 taaaatcgcc tctccgtgtg gagaggcgca ggagattacg cgtccgggaa ctcacagata
    16921 aagcgttcga catcttcaac catatggtcg ttgccgacaa agaatgaacg gcgctggtgc
    16981 agggtttccg ggataatatc cagaatacgc tctttgccat cgctcgcttt accgcccgct
    17041 tgttccgcca ggaatgccat cgggttgcac tcatacagca aacgcagttt gccgtccggg
    17101 tggctggcgg tgcttgggta gagataaata ccgcctttca gtaggttacg gtggaaatcc
    17161 gcaaccagtg agccgatata acgtgaggta tacgggcggt tggtggattt atcttcttcc
    17221 tggcagaatt taatgtactt cttcaccccg ttcggaaact taatgtagtt tccttcgttg
    17281 atggagtagg ttttgccttt ctccgggaag cgcatccgtt cctggcacag gcagaaaacg
    17341 ccgagcgaag gatcgtaagt aaaagcgtga acaccgcatc cggtactgta aaccagcatg
    17401 gtagaggagc cgtataccac gtaacctgcc gcaacctgtt tgttaccagg ctggaggaaa
    17461 tcttcttccg ttaccggcgt gccgacgggc gtaactcggc ggtagatgga gaaaatggta
    17521 ccgacagaga cgttaacatc gatgttggac gagccatcca gtgggtccat cagcaccacg
    17581 tattttgcat gttcacagcc ttcaaagaca acaatctcat cttcttcttc agaggcaatg
    17641 cctgcaacga tatcgcgtgc tttcagtgcg gctttcagtt tttcattagc gaacaagtcg
    17701 agtttttgct gaacctcgcc ctgcacgttc tcagcaccgc tggcacccag gatatcaacc
    17761 agtcctgctt tgttgatatc gcgatggata atcttggcgc ccagttttat tgccgacagc
    17821 aaagcagtga gctcaccggt agcatgagaa aactcgtgct gcttttcgac aataaattca
    17881 cctaacgttt tcataaaact ttccctgcaa tgtttatgga gtaaagcgac cgcaacaatc
    17941 ttaacaaata atctcaatgt tgcgctcagg tgaatcgcgc cagcaaatta cggattatcc
    18001 tgaaatgcgt ttctcacttg cccgacatat gcgtaaaatg agcggcagat taaaaaagga
    18061 tagtgacgca tgcgcattca tattttagga atttgtggca cgtttatggg cggtctggcg
    18121 atgctggcgc gccagttagg ccatgaagta acgggttcgg acgccaatgt gtatccgccg
    18181 atgagcacct tacttgagaa gcaaggcatt gagctgattc agggttacga tgccagccag
    18241 ctcgacccgc agccggatct ggtgattatt ggcaacgcca tgacccgtgg aaatccgtgt
    18301 gtggaagcgg tactggaaaa aaacatccct tatatgtcag gtccacagtg gctgcacgat
    18361 tttgtgctgc gcgaccgctg ggtgctggcc gttgccggta cacacggcaa aaccaccacc
    18421 gcgggaatgg cgacttggat tctggaacag tgtggttaca aaccgggctt tgtaatcggc
    18481 ggtgtgccgg ggaactttga ggtttcggcg cgtctgggcg aaagcgactt ctttgttatc
    18541 gaagcggatg agtacgactg cgccttcttc gacaaacgct ctaaatttgt ccattactgc
    18601 ccgcgtacgc tgatcctcaa caaccttgag ttcgatcacg ccgatatctt tgacgacctg
    18661 aaagcgatcc agaaacagtt ccaccatctg gtgcgtatcg ttccggggca gggtcgtatt
    18721 atctggccgg aaaatgacat caacctgaaa cagaccatgg cgatgggctg ctggagcgag
    18781 caggagctgg tgggtgagca gggtcactgg caggcgaaaa agctgaccac cgatgcttcc
    18841 gaatgggaag ttttgctgga tggcgaaaaa gtgggcgaag tgaaatggtc gctggtaggc
    18901 gaacataata tgcacaatgg cctgatggcg attgcagcgg ctcgccatgt tggtgtagcg
    18961 ccggcagatg ccgctaacgc gctgggttcg tttattaacg ctcgccgccg tctggagttg
    19021 cgtggtgaag cgaatggcgt cacggtatat gacgattttg cccatcatcc gacggcgatt
    19081 ctggcaacgc tggcggcgct gcgtggcaaa gttggtggta cggcgcgcat tattgctgtg
    19141 ctggagccgc gctcgaatac catgaaaatg gggatctgca aagacgatct ggcaccttca
    19201 ttaggtcgtg ccgatgaagt cttcctgctg caaccggcgc atattccgtg gcaggtggca
    19261 gaagtggcag aagcctgcgt tcagcctgca cactggagtg gcgatgtgga tacgctggca
    19321 gatatggtgg tgaaaaccgc tcagcctggc gaccatattc tggtgatgag caacggcggt
    19381 tttggtggga tccatcagaa actactggat ggtctggcga agaaggcgga agctgcgcag
    19441 taattcggcc tcagcctgag atagtgtgcg taggccggat aaggcgttca cgccgcatcc
    19501 ggcatttgag catagtgcct gatgcgacgc ttgatgcgtc ttatcaggcc tacaggtgca
    19561 ccgcatccgg caaaccatac tcacatcaac aacgaaaatt acccttcatt ctccgccagt
    19621 tcgcgcagat actggaaaat ctggcgtgcg gatttcggcg gcttattccc ttctttctct
    19681 ttcttcgcgt tgcggatcag agtacgcagt tgctgacgat cggcatcggg ccacagattt
    19741 aatacctcag cgatggcgtc atcaccttga tcgatcagac gatcgcgcag gttttccagt
    19801 ttatggaaca gcaccacctg ctggttgtga cggtttttca gcttatccag cgcctgacgg
    19861 atcggttcta catcgcgctg gcgcagcatc ttaccgatga gctgcaactg gcggcggcga
    19921 ccttccatct taatacgctg ggcgagctca atagccgcgc gcagatccgc atctaacggg
    19981 atcttatcca gcgcgttttt ccccagatca acaatttccg cgccaaggcg ttttagctcc
    20041 tcggcatcac gtttaatttc acttttactg acccagataa tttcatcgtc ttcgtcttcg
    20101 atgtcatcac cgggaacgtc gtcgagccag tcttcgggct gcttagtcat ctcaggctcc
    20161 ttaaaaaaag aggctaatgt taccagttaa gatgcgcact gaaaaacggt tctctgttag
    20221 acttcagaga aactctctac attatggcac ttgcaatgaa agtaatctct caagttgaag
    20281 cgcagcgcaa gattctggaa gaagcagttt ccactgcgct ggagttggcc tcaggcaaat
    20341 cggacggtgc ggaagttgcc gtcagcaaga ccaccggcat tagcgtaagc acgcgttatg
    20401 gtgaagtgga gaatgttgaa ttcaatagcg atggcgcgct ggggatcact gtttatcacc
    20461 agaaccgcaa aggtagcgca tcatccaccg atttaagccc gcaggccatt gcccgtactg
    20521 tacaggcggc gctggatatt gcccgttata cctcgccaga tccctgtgcc ggcgtggcag
    20581 acaaagagct gctggccttt gacgcaccgg atctcgactt gttccaccct gcggaagttt
    20641 ccccggatga agccattgaa ctggcggccc gcgcagaaca ggcggcattg caggcggaca
    20701 aacgcatcac caataccgaa ggtggcagct ttaacagcca ctacggtgtc aaagtttttg
    20761 gcaacagcca cggcatgttg cagggttact gctcaacgcg tcattcgctc tccagctgtg
    20821 taattgccga agaaaatggc gatatggagc gtgattacgc ctacaccatt ggccgcgcga
    20881 tgagcgattt gcagacgcca gagtgggtcg gggccgattg cgctcgccgt actttatcgc
    20941 gtctgtcacc gcgtaaactc tccaccatga aagcgccggt catttttgcc aatgaagtgg
    21001 caaccgggct ttttgggcat ctggtggggg cgatagcggg tggatcggtt taccgtaaat
    21061 ctaccttcct gctggattcg ctgggtaaac aaattctgcc ggactggctg accattgaag
    21121 agcatccgca tctgctgaaa gggctggcat cgacgccatt tgacagcgaa ggtgtgcgca
    21181 ccgagcgtcg cgatattatt aaagatggca tcctgactca gtggctgctg accagctact
    21241 cggcgcggaa actggggctg aaaagcaccg gacatgcggg cggtattcac aactggcgga
    21301 ttgccggaca aggtctaagc ttcgagcaga tgctcaaaga gatgggcacc gggctggtgg
    21361 tgacggaatt gatgggccag ggcgtgagtg caattaccgg tgattattcc cgtggtgcgg
    21421 cgggcttctg ggtagaaaac ggcgaaattc agtatccggt tagcgaaatc accatcgcag
    21481 gtaatttaaa agatatgtgg cgcaatattg tcaccgtcgg taacgatatt gaaacacgca
    21541 gtaatataca gtgtggttct gtgctgttgc cggagatgaa aatcgccgga cagtaaaaaa
    21601 actggcgcga cctgccgcgc tataaactaa aaaattacac aaaatcattc gcactgcatc
    21661 gagacggaaa gtgaatgagg gccggggagc gtactcgcag tacgtgaccc ggtcgaatga
    21721 gcgtagtcgt cgatgaggca gtgtgaagga tgaagtgtaa ataaaaaagg aagtgagcaa
    21781 tgcgaaaaag cctgttagct attcttgcag tctcctcgtt ggtattcagt tcggcgtcgt
    21841 ttgctgctga tcttgaagac aatatggaaa ccctcaacga caatttaaaa gtggtcgaaa
    21901 aagccgataa cgcggcgcaa gtcaaagacg cgttaacgaa gatgcgcgcc gcagcgctgg
    21961 atgcgcaaaa agcaacgccg ccgaagctcg aagataaatc accggacagc ccggaaatga
    22021 aagatttccg ccacggtttc gacattctgg tcggtcagat tgacgacgcg ctgaagctgg
    22081 caaatgaagg taaagtaaaa gaagcgcagg ctgctgcaga gcaactgaaa acgacccgca
    22141 attcatatca caagaagtat cgttaattcc tcatttccct gttgcctgca ctcaggtaac
    22201 agggaactta tcatcgcaaa tgatgcacca cctgattgct gctgccgcgc cagatcaggg
    22261 aagggtcttt taaatcctgc acaaatttgc cgtcgaccag cacgttaatc aaatcaacaa
    22321 cctgcatttg cgcagcgttg agttcgtcga gtttatagcc tgtccacacc cagatgtctt
    22381 tacccggaca ctcggcgcgg atgcgttgta ccagtttcag aatatccggc acgttttgcg
    22441 gatgcagcgg atcgccgccg gagagggaaa taccctggcg tttgatacga gtgtcattca
    22501 gatcgttaat gatctggtct tccattgctt tagtaaatgg ctgaccggaa tttacccgcc
    22561 atgtgctttt gttatagcaa ccggggcatt catgaacaca cccggagaca aacagggtgc
    22621 agcgagtgcc gggaccgttg acgatgtcga caggatagta ctgatgataa ttcattattt
    22681 cgcctggctt atcagtccgc gtgtaggctg tatcaggcaa tcaacgcctg atgcgacgct
    22741 gtcgcgtctt atcaggccta cagccgttgc caaatgtagg ccggataagg cgtttacgcc
    22801 gcatccggca atcaacgcgg gatgcgacgc tttcacatca catccggcat ttgcttagtg
    22861 cagatcaacc tatctgccca tttcccaaat gtttaacgcg gcgcttaact tcttcctgct
    22921 tgccggcgtt aaacggacgt gcatccgggc tacctaaata tccgcacacg cggcgagtta
    22981 ccgacacacg ggaggcgtca tggttaccac atttcgggca ggtgaagcct ttgctggtgc
    23041 actcgaactc accggtaaag ccacactcgt agcactcatc aatcggtgta ttggtgccgt
    23101 aatacggtac atgctgatag ctgtaatccc agacatcttc cagcgccttc aggttgtgct
    23161 gaatgtttgg atactcgccg tagcaaatga aaccaccgtt cgccagcggc gggtaaggcg
    23221 cttcaaagtc gatcttgtcg tacgggttca ccttcttctc cacatcgagg tggaaactgt
    23281 tggtgtagta acctttatcg gtcacgcccg gcaccacgcc aaactcagca gtatcgagac
    23341 ggcagaagcg gtcgcacagg ttttcactcg gcgtgctgta gagactgaaa ccataacccg
    23401 tttcttcttt ccactgatcc actgcctgac gcagacgttc aacaatggcg atacctttcg
    23461 cgcggagctg ctcgttgtcg taaacatgtt cgccgccgaa cagcgcgtta atggtttcgt
    23521 ggatgccgat gtaacccagc gaaatagacg cacgaccgtt tttgaagatt tcagaaatat
    23581 catcgtcagc attgagacgc acgccacaag caccttccat atagaggatc ggggccacgc
    23641 gcgctttcac gccttcgaga cgagcgatac gggtcatcag agccttacgt gccagcacca
    23701 gacgttcatc cagcagcttc cagaaggtgg cttcatcgcc ttttgcttcc agagcaatac
    23761 gcggcaggtt caggctgatc acgccgaggt tgttacgacc atcgtggatc tgctcgccgt
    23821 tttcattttc ccacacgccg aggaagctgc ggcagcccat cggggtttta aacgaaccgg
    23881 tgactttcac tacctgatcg tagttcagga tatccggata catgcgcttg cttgcgcact
    23941 ccagcgccag ctgtttgatg tcgtagttcg gatcgccttt tttatggttc agaccatcgc
    24001 gaatcgcaaa caccagtttc gggaacaccg cagttttacg gtttttaccg aggcctgcga
    24061 tacggttacg caggatagat tcctgaatca ggcgcgactc ccagctggtg cccagaccaa
    24121 aaccaaaggt tacaaacggc gtctgaccgt tggcggtgtg cagggtgttt acttcgtact
    24181 ccagcgactg gaaggcatcg tagcactctt taatggtacg agagttagcg tagccttcgg
    24241 cgtccgggat gttccactct tctgcggtct tgcgatgttt gttgtagctg gcggtgacaa
    24301 acggtgccag cacttcatcg atacggttaa tggtggtgcc gccataaata tggctggcaa
    24361 cctgagcaat aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc ggcggttcaa
    24421 tctcggcgtt ccccatctta aagccctggg tcagcatgcc tttcaggtcg atcaacatgc
    24481 agttgaacat cgggaaaaac ggtgagtaat cgagatcgtg atagtgaata tcgccacgct
    24541 catgtgcctg caccacgtca cgcggcagca ggtgctgacg tgcatagtgt ttagccacga
    24601 tcccggccag caggtcgcgc tgggttggaa tcaccttgct gtctttgttg gcgttttcgt
    24661 tgagtaacga ggcgttggtc tgctcgacca gaccacggat ctcctgattc aggcgaccgc
    24721 gtttttcacg ttcaatgtcg cgatcgtgac ggtactcgat gtaagcacga gccagttgtt
    24781 tgtatggacc tgacatcagc tggttttcaa cagcggtctg gatctcattg atatccacct
    24841 ggttgcggcc ctgcatctgc tcgctgacaa ctgcagcaac agtggcgcaa taatcggcat
    24901 catcgacttc cgctgcttta gctgcacgca gaatcgcttc tttgatgcgc tctgatttaa
    24961 acggcacttt gcagccgtct cgtttcatca catgcggtgt catgatcgct ccatattttt
    25021 aagaacaggt tatccacaga aattgggaaa ggcgttttcc ggttttttcg tttcgttttc
    25081 cggtgctttc cgcaatcgcc atccgcttat ccacaacgcc gcaccttttt cgtggggagc
    25141 tgtagtagca attatagtcg attgatacaa catattgggt tgggacgcat tttaaagtct
    25201 atatatagtg ctttgcatca aggatgtttg aacttttttt gatgtagctc aaagtaaaaa
    25261 gcagagcgta cggatgacgg gcgctacagc gttatgtaaa ttttttaatg aatttgctgg
    25321 ttgaaaaatc aacaaaaaca acatgctgac agacaaaacc ccggaatgac cggcattacc
    25381 ggggcttagg gaagatttac ttctgtaacc accagacagc ctcaaaaggc cgtaaagtca
    25441 tggcacaggg ttgtggtgag gcttcttcgt agttatgcat cacaagctgc cagttgccgc
    25501 gcatttgccc tggctgccag ggttggatct cacggctaag gttggcaatg accagcaagg
    25561 tttgcccctt ccattcacgg cgatagcacc acaatacagg gctgtttggc agcagatcct
    25621 ggtaattgcc ccatgtcagg atggcttcct gcttacgcag tgcgattaac ttttggtagg
    25681 tgtaaaacac cgaggactca tcggccagcg cggcttctac gttgatttgt tgatagttat
    25741 cgcccaggcc aatccacggt tcgccagccg taaacccggc attatcgccg ttgctccatt
    25801 gcatgggcgt gcggctgttg tcacgggatt tactggcgag gattgccaat aactcgtcgg
    25861 catcacgccc atcgttgcgc agctcggcaa acatattgag gctctctacg tcgcgatagt
    25921 cagtaatgcg cgtgaaatgc gggttggtca tgccaatctc ttcgccctgg tagatatacg
    25981 gcgttccctg catgccatgc agcaccatcg ccagcatttt tgccgcaggc acgcggtatt
    26041 caccttcatc accaaagcga gaaacaatgc gcggctgatc gtggttacac cagaacaagg
    26101 cattcaatgc tacgttgtgc attccttgtt gccagtggcg gaacaatgtt ttcaacgcca
    26161 caaagtcagg tttagccagc gtccattttt caccaccggg ataatcgacc ttcaggtgat
    26221 gaaaattaaa ggtcatcgac aattcactgc ctgtcagtgc cgcgtatcgc tggcaatgct
    26281 caaggctggt ggaggacatt tcacctacgg tcattaaccc gcgtggcgta aacacatcgc
    26341 ggttcatctc gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag cgacgcccgt
    26401 cgccatccag gtcatcaggg aaacgcgggt ctttggagat cagattcacc acatccaggc
    26461 gcaacccgtc gaccccacga tcggcccaga actcacagac ttttttcagc tctgcgcgta
    26521 ccgctggatt ctcccagttg agatccgcct gttctggtgc aaagagatgc aaatagtact
    26581 gttcgctttc cgcatgccag cgccacgcac taccgccaaa ttttgaacgc cagttgttcg
    26641 gtggcgtttc tggttcgcca tcgcgccaga tataaaactg gcggtaaggg ctttctttgt
    26701 tcagcgcctc gcgaaaccag gcatgttggg tagaggtatg gttaaacacc atatcgagaa
    26761 tgatacgaat cccgcgcgat tttgcctgcg tcaccagttc gtcaaaatcg tccagcgtgc
    26821 cgtaggtggg atcaatcgcc gtatagttcg ctacatcgta accgttatcg acctgcggag
    26881 agacataaaa gggggttagc cagatggcat caacgcccag tttatgcaga tagtccaggc
    26941 gttggataac gccacgtaaa tcgccggtgc cgctacccgt ggtgtcctga aaactctttg
    27001 gataaatctg gtagataacg ccgttttgcc accagtgggg aagattagtc attacgttat
    27061 tcctgcaaat gcgagggggc gcaattgcgc cccgaagaaa attaaacaat gtccagcgtg
    27121 cccaggcggt atttccgctg atagataaac gaggtgagta caatcgggat gatgatggcg
    27181 atagccattg ccagcgcaaa cacctgccag tagctcggtt gaatcgagag aattcccggc
    27241 aggccgccta cgccgatgcc attcgccata acgccgttca ggccgcatag caatcctgcc
    27301 agaccagaac caatcatcgc gcacagcatc gggaagcgat atttcaggtt gatgccgtac
    27361 attgcaggct cagtgacccc aagccaggcg gagatcgccg caggcacgga gatctcgcgt
    27421 tcattgtgct tgcggctgga aatgataatg cctatcacgg cggagccctg agcgatattc
    27481 gacagcgcaa tcagcggcca cactggcgta ccacccatgc tttgaatcat ctgcaaatca
    27541 atagcaagcg tggtctggtg tacaccggtg atcaccagcg gggcgtacag gaagccaaac
    27601 aatgccgcgc caatcggagc aaagctgccg gtcatcaggt gacgtaccgc aaaggcaacg
    27661 ccatcgccaa tcatgcgacc aaacggacca atcagcgcat gggcgaggaa caccgcgagg
    27721 atcagcgaac agacgggcac caccaccaga tagaggtaat ccggcacgat gcgtttaagg
    27781 cgagtttcaa taacgcccag cgccagcccg gctaacagtg ccgggatcac ctgcgcctga
    27841 tagcccactt tggcgatgct gaacatgcca aagtcccaca cttccggcag ctgctgcccg
    27901 agcagataag cgttcatcag ttggggggaa accagtgtca cgccaagcac gataccaagg
    27961 atcggcgtgc cgcccatttt tttcaccgct gaccagcaaa tcccgaccgg caggtagaag
    28021 aagatcgctt caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga agggtacatt
    28081 tgcgccagcg tttgaccgtt gctcatgggc aaatcgccga tcacattgcg aaaaccgagg
    28141 atcaaaccgc cgctaatcaa cgcgggcagc aacgggaaga agatctctgc gaaatgagag
    28201 atcaactgct catgccattt catattctgc cgggcggctt tttttacctg ctctttatca
    28261 acctgcgcct gtccggttga cgctatcagt gcttgatagt aatcacccac gttggtgcca
    28321 atcaccacct gaaattgccc ggcattggtg aaacagcctt tcaccatggg gagttgctca
    28381 atttctttcg gtctggcatt ggccggttgg ttgaggacaa agcgcaggcg agtaatacag
    28441 tggctcaccg tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg atcgatatcc
    28501 gtttggttta ttttgctcat cataaagccc catggcagat gacatttttg gttggctgca
    28561 gaatatattg cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacgcag cgaagatcac
    28621 aatttatcgt tcaggaaacg atcaggacag gttggcgggg atgatgattt gttgcggttc
    28681 gctgcgtccg gttacctgcg cgatcaactg gcaagccgcc tggcgtccag cttcggcgta
    28741 accaggatct acggtgacga tctccggatg aaggaatttc attaacggcg tattaccgac
    28801 gctcgccagt tgcaaggtgt cgatgcgttg ctcttgcagg tatttacttg cgccaagtgc
    28861 cagcgtgtcg gttgcgcaca gtaaggcggt agtttcaggc gtaatcactt ttgctacgtt
    28921 ctcatagcct tgcttcatag caagccctgg cagggcggca acgggatgca gtttatgcgc
    28981 tttgcagaac gccaggtagg cttcgtgacg tcgcttaccg gttgtcacgt cactgtgcgg
    29041 cacgccgaga taactgatat tacgatgccc ctggtcatac agccgttgca tcaggatctt
    29101 gattgcccct tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac gcgccagcag
    29161 aaccagcgat gactgccagt gggctaacat ttcttctgtt atgccagtaa aaccgaacag
    29221 cactacgccg tcgatattac gccgtttcag cacccccaaa tgttcggcaa ctaattgcgg
    29281 ggaaaactga ctttccatca tgattgggtc gtaaccttgt tcatagaacg ctggcagcat
    29341 ggtttgaacg gcgagatttt ctgacaacga atccagacgg gtaacaatga tggcgaccac
    29401 tttatcgctt tgcccacgca tagcgcgcgc agaacgggaa ggggaaaatc catgctgatt
    29461 catcactgct tcaacacgct cgcgggtgcg ctggctcacg ccgctttcgt tattcagcac
    29521 ccgggaaact gtagatttcc ccacgccgct taagcgcgcg atgtctttga tggtcagccg
    29581 attttgcatc ctgttgtcct gtaacgtgtt gtttaattat ttgagcctaa ctttacccgt
    29641 gcattcagca atgggtaaag tctggtttat cgttggttta gttgccagca ggtattatat
    29701 cgccatagat gctacgaata ttattggatt ctccttatta tttgcggcgc ttttttcact
    29761 taccggaggt tatatggaac ctgatcccac gcctctccct cgacggagat taaaactttt
    29821 ccggtaagcc cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg ttttaacgtc
    29881 cctgctcagc tttattacct tcaggtaagg cttcgccacg cctgaagaca tttctgtact
    29941 gtttcagaca gtgcggaggg actccttatg tttaaagaaa tttttacccg gctcattcgc
    30001 catttacctt cccgtctggt tcatcgtgat ccattgcctg gcgcgcagca gacagtgaat
    30061 acggtggtcc cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat gcccgaagaa
    30121 gaattgtgga aaacgttcga cacccatccg gaagggttaa atcaggcgga agtggaatct
    30181 gcccgcgaac aacatggtga aaataaatta cccgcacaac aaccgtcgcc gtggtgggta
    30241 catttatggg tctgctatcg caaccccttt aatattttac tcaccattct cggcgctatt
    30301 tcttacgcca cggaagattt atttgccgcg ggcgttatcg cgctaatggt cgctatttca
    30361 acgttgctga actttattca ggaagcacgt tccactaaag cggcagatgc cctgaaagcg
    30421 atggtcagca ataccgcgac ggtgctgcgc gtaattaacg acaaaggcga aaatggctgg
    30481 ctggagatcc cgatcgacca gctggtgccc ggcgatatta taaaactggc ggcgggagat
    30541 atgatcccgg cagatttacg tatcttgcag gcgcgggatc tgttcgtcgc tcaggcgtcg
    30601 ttaaccggtg agtctctgcc cgtggaaaaa gccgctacca ctcgccagcc ggagcacagc
    30661 aatccgctgg agtgcgacac gctgtgtttt atgggcacca ccgtggtgag cggcacggca
    30721 caagcaatgg tgattgctac aggtgccaat acctggtttg gtcaactggc ggggcgtgtt
    30781 agtgagcagg aaagcgagcc gaatgccttt cagcaaggga tcagccgcgt cagtatgctg
    30841 ctgattcgct ttatgctggt gatggcaccg gtggtgctgt taatcaatgg ctacaccaaa
    30901 ggcgactggt gggaagcggc gctgtttgcg ctttcggtcg cggtaggcct aacgccggaa
    30961 atgttgccga tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct gtcgaaacag
    31021 aaagtgatcg tcaaacatct ggatgctatt cagaactttg gcgcaatgga tattctgtgc
    31081 actgataaaa ccggcaccct gacacaggat aaaattgtgc tggagaatca taccgatatc
    31141 tccggtaaaa ccagcgaacg cgtgctgcat agcgcgtggt tgaatagtca ttatcagacc
    31201 ggacttaaaa acctgctcga tacagcggtg ctcgaaggta cggatgaaga gtcagcgcgc
    31261 tcgctggcca gtcgttggca aaaaatcgat gagattccgt ttgatttcga gcgtcgccgg
    31321 atgtcggtgg tagtggcaga aaataccgag caccatcagc tggtttgcaa aggtgcattg
    31381 caggaaatcc tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt gccgctcgat
    31441 gacatcatgc tgcgtaagat taagcgggtt actgatacgc tgaatcgtca ggggctgcgc
    31501 gtggttgcgg tggcgacgaa atacctgcca gcgcgtgaag gagattacca gcgggcggat
    31561 gaatccgacc tgatcctcga aggatatatt gctttccttg atccgccaaa agagacaacc
    31621 gctccggcac tgaaggcatt aaaagcgagt ggaattaccg taaaaatcct tactggcgac
    31681 agtgagttag ttgcagcgaa agtgtgccat gaagtggggc tggacgcggg agaggtggtc
    31741 attggtagtg atattgaaac gctatctgac gacgaactgg caaatcttgc acagcgcact
    31801 acgctgtttg cccgcctgac gccgatgcat aaagaacgca ttgtgacctt actgaagcgc
    31861 gaagggcatg tggttggctt tatgggcgat ggtattaatg atgcgcccgc cttacgcgct
    31921 gcggatatcg gcatttctgt ggacggcgcg gtagatattg cccgtgaagc ggctgatatc
    31981 atcctgctgg aaaaaagcct gatggtgctg gaagaggggg ttattgaggg acgtcgcact
    32041 ttcgccaaca tgctgaaata catcaaaatg acggcgagtt ctaacttcgg taatgtgttc
    32101 agcgtgctgg tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt acacttgctt
    32161 attcagaacc tgctgtacga tgtgtcacag gtggcgatcc cgtttgataa cgtcgacgac
    32221 gagcaaattc aaaagccgca gcgttggaat ccggcggatc tggggcgctt tatgatcttc
    32281 ttcggaccga tcagctcgat cttcgatatt ctgacgtttt gcctgatgtg gtgggtattc
    32341 catgccaaca cgccggaaac gcaaacgctg ttccagtcgg gatggtttgt ggtgggctta
    32401 ctgtcgcaaa cgctgattgt gcatatgatc cgcacccgcc gtgtgccgtt tattcagagc
    32461 tgtgcatcgt ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg gatcgcattg
    32521 ccgttttcac cgctggccag ttatctgcaa ttacaggcgc tgccgttaag ctatttcccg
    32581 tggctggttg cgattctggc agggtatatg acattaaccc agttggtgaa agggttctat
    32641 agccgtcgtt acggctggca ataaagaata aactgggcac gatagccccg attcagattg
    32701 cagacaaagt aagctttgtt catgccggat gcggcgtaaa tgccttatcc ggccaacaaa
    32761 ttcaataaat tgcaggaatc acgtaggcct gataagcgta gcgcatcagg cgattttgcg
    32821 tttgtcatca gacttaatcc gggcatgata gcccggattt ccatcaagat tagcgacgaa
    32881 cagcgatcgc ttcgatctca atcttcacgt ctttcggcag acgggcaact tcaacgcaag
    32941 aacgtgccgg gaaggtggcg ttgtgttcgg tgaagaaggc ttcgtaagtg gcgtttacgg
    33001 ttgcgaagtc gttcagatct tttacaaaca cggtagtttt aacgatgtcg cccactttca
    33061 gaccagcggc ttcgacgatc gcttttacgt tatccagcga ctgacgtgcc tgtgcagcga
    33121 cgtctgccgg tacttcgccc gttttcggat ttaccgggat ctgaccggaa gtgatgatca
    33181 tattgcccag atcaacgccc tgtacgtaag gaccgatagc tgccggtgca ttttccgtcg
    33241 cgatagtttt gctcatgatt tctcctttat tacagcggta aaaagtctgg ctcattatag
    33301 ggagccagac ttttattacc aaccgcaatt aattggccag caccacatta tgggaaaact
    33361 ctttttcaca gtatttgcat ttgagcgcga tatcattggc gcgttttcgc acggcaaagc
    33421 tggaagaaac cggttcggca tggctgatac agttgctgtt cgggcagacc agcacattgt
    33481 cgatgcgctc cggcagactt gggcgcgatt tacccaccac ttcatagttg tcgatacggt
    33541 taaccgtggc ttgcggcgca tacaatgcca gttgatctac ctggtcttca ctcaaaaagg
    33601 tattttcgat tttgatcaga tctttgcggc ccatctcgcc agaaggcagg ttcagaccga
    33661 tggtgatgcg ctgatccgtt tcggtcagct tgaacagact caacagctta aaaccgatct
    33721 gggcggggat atggtcaatt accgtgccgc gtttaatagc ttcaacctgc aatttattat
    33781 cgtgtgtcat ctctatttcc ccttacagta ccagatcgcg attcagaacc agtgccagta
    33841 acgcctggcg agcgaaaatc ccgttgcctg cctgctggaa gtaccaggcg tgtggcgttt
    33901 tatcaacatc cgtcgcaatc tcatcaacac gcggcagcgg atgaagtact ttcatattgg
    33961 ctttggcgtt gtggagatcg ctggcgcgaa gaacaaactg cgctttcacg ttggcgtact
    34021 cggacgggtc cagacgctct ttttgcacgc gggtcatgta caggatgtca acttccgcca
    34081 tcacttcttc aatagagctg tgcagactcc atgcgatccc tttttcatca agcatatcca
    34141 gaatgtattg cggcattgcc agcgcgtccg gcgcgatgaa gtaaaaacgg ttgccgtcga
    34201 acttcgctaa cgcctgagtc agggagtgaa cggtgcggcc atatttcagg tcaccaacca
    34261 ttgcgacgtg gagattgtcc agacgcccct gggtttcctg aatggtgaat aagtccagca
    34321 aggtttgcgt cggatgttgg ttggagccat caccggcatt cagtaccggt acattgccgg
    34381 aaaactcggt ggccaggcgc gccgcacctt cctgcggatg acgcatcact atcgcatcga
    34441 cgtaagtgct gataaccgaa atggtatcgg ccagcgtttc gcccttttta cccagtgatg
    34501 tattggcgct gtcggagaag cccaccacgc tggcacccag gcggtgcatg gaagtttcga
    34561 aagagaggcg ggtacgggta gaggcttcga agaaacagct ggcaatgact ttgtgcttca
    34621 acagctctgg ttgcgggttt gctttcagtt tcgccgctgt cgccagcacc agattaaggt
    34681 catcgcgact aaggtcgttt atggaaatga tatgtttctg atatagcgga ttagccatct
    34741 tttatctcct gacgcctggg caaaaaaaag cccctcgatt gaggggctgg gaatgggtga
    34801 tcaacgggaa gaaaaacggc aggccagcgt cttttttcag acgcggtaag acaaaatgtc
    34861 gaacacactg aaccatacat cctcccggca aattgtccgg cattatactc atcgtcagaa
    34921 gcggtgcaag catttgatgc aatattttgt cagcgcaaac ggtttatttg aattataagt
    34981 caaaatatat gtatttttat ttactgtgat tctggagggg aactgtcgtt cggtatcaat
    35041 aattgcagac atttcctgct tttttctttt tcactttcac gcaatcagat atgcatttta
    35101 ttccattcat ccgacttata gggcggagtg tgaaaacgaa cggctaacac tattgcttac
    35161 agctcaggga tgcgcgctat cactttaatt tcaaaatcaa agcctgccag ccatgtaacg
    35221 cccaccgccg tccaggttgg ataaggtggc gcgctaaata tttcattttt caccgtcatg
    35281 atgtcttcaa attgtttttc tggatcggta tgaaagctcg taacatcaat gatatcgtca
    35341 aaagtgcatc ccgcagctgc cagggtcgca tgcaaattat caaatgccag tctgacttgt
    35401 tgctgaaaat cgggttctgg tgttccgtcc tctcgacttc ctacttgccc ggaaacaaac
    35461 agcaaatcgc cggaacgaat agccgcagaa taacgatgtt ctgcatacag tgaatgtcgg
    35521 ccagcaggga aaacagcggt tctttctacc atttggttat cctcaagatt tacgacatga
    35581 acagaagatt tatatttacc aggagccgct tttagcggac gacgtgagta aacaaaaccc
    35641 agacatcatg ggaaatggct gggcttaatt gagcgtagtc ggttatgcgc caaacgcgcc
    35701 atcaatggta tgcatcgcgc cggtaacaaa actggcttct ggccctgcta accatgcgac
    35761 cataccagcg acctcttccg gttgcccatg tcttttgata gccataaaac tatgcaacat
    35821 atcgcgcatt ggcccgttgg cgggattagc gtcggtatca attggccctg gctggacgac
    35881 gttgatggtg atcccacgcg gtccaaaatc acgggccagc ccgcgcgcca tgccttgtag
    35941 ggcagattta ctggcggcat aagcagccat gcctgcaaca ggcatacgat cgccattcac
    36001 ggagccgatg attaagatgc gcccaccttc gggcatctgc cgggcggctt caacagaggc
    36061 atggtaagga gcatgaatat tgattttgaa aaggcgatca atatcgtcgg catttaattc
    36121 cagggcatcg ccaaagacgc caatacctgc atttaccacc aggatatcca atgcgccgct
    36181 cttacgaacg acatcaatga cagcgtctct gtcagcacta tctgtaaata ctgctgtcgc
    36241 tcccgtctct tgtgccaggc gttcagcggc atctttcgac cccgcatagg tgaatcgtac
    36301 attggcccca tcggtgacga aacgacgtac gatagcggca ccgattccac gactgccacc
    36361 gaggatgaga actgtcttac ctgtaaaagc gcccataagg actccttgat ttattatgta
    36421 acatgcatta caaaactgtt ttaactttct gtcaacaagt tttgtaatgg tcactaaaaa
    36481 acaatctcgc gttccaggtc gtcccagacg tttcgctcct gagcaggcag tctctgcggc
    36541 aaaagtgctt tttcaccaaa aaggtttcga tgctgtcagt gttgctgaag ttactgatta
    36601 tcttggtatt aaccccccga gcctctacgc ggcttttggc agtaaggctg ggttatttag
    36661 ccgtgtactc aatgaatatg tcggtacgga agctattccg cttgtcgata ttcttcgtga
    36721 tgatcgtccg gtaggggagt gcctggctga ggtattaaaa gaagcggcgc gcagatatag
    36781 ccaaaacggc ggctgcgctg gctgtatggt tcttgaaggc attcatagtc atgatccaca
    36841 agcgcgtgat attgccgttc aatattatca cgccgcagaa acggccattt atgactatat
    36901 cgccaggctg catccacaaa gcgcacaatg tgtgactgat tttatgagta ccgtgatgtc
    36961 agggctttct gcgaaggcac gagaggggca ctccctcgaa caactctgtg caactgctgc
    37021 actggcgggg gaagcgataa aaactattct caaggagtga tgctggcctt gatccgaaag
    37081 gcgggaacgc gcctgccgat aagttgtgat aagacaataa ttcacgcgtt aaggctggct
    37141 gaattgatca tcttttcgta taacggtgga aatgaaacat tgttttaatt aaggagtgga
    37201 aaagatgatc atcggaaata ttcataatct tcagccgtgg ctaccccagg agttacgcca
    37261 ggcgattgag catatcaaag cacacgttac ggcagaaacg ccaaagggca agcacgatat
    37321 cgaaggtaat cgtctgtttt atcttatctc ggaagatatg accgagccgt acgaagctcg
    37381 ccgtgcggag taccatgccc gctatctcga cattcagatt gtgttaaaag gtcaggaagg
    37441 catgaccttc agcacacaac cggcaggtac gccggatacc gactggttag ctgataaaga
    37501 catcgcattt ttgccggaag gcgttgatga gaaaacagtt atccttaatg aaggtgattt
    37561 tgttgtgttt tatccggggg aagtgcataa accgctgtgc gcagtgggtg caccagccca
    37621 ggttcgcaaa gcagtagtga agatgctgat ggcgtgatga cttttcgccg taaataactc
    37681 caggtttacg gcgagtttgt gaaaagagcg ttttttgata tttttttgtg agtaaaattt
    37741 gtaatgctta gacgttctta ttaactcaag gagttcgtca tgagcaaaat atcagattt
//
LOCUS       DAZFDC010000029        37572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFDC010000029 DAZFDC010000000
VERSION     DAZFDC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.029"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            210..1583
                     /gene="aroP"
                     /locus_tag="ACHJ48_001636"
     CDS             210..1583
                     /gene="aroP"
                     /locus_tag="ACHJ48_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO4707214.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVTVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAVVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1626..2480)
                     /gene="ampE"
                     /locus_tag="ACHJ48_001637"
     CDS             complement(1626..2480)
                     /gene="ampE"
                     /locus_tag="ACHJ48_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO4707215.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFRTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2477..3028)
                     /gene="ampD"
                     /locus_tag="ACHJ48_001638"
     CDS             complement(2477..3028)
                     /gene="ampD"
                     /locus_tag="ACHJ48_001638"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO4707216.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3116..4009
                     /gene="nadC"
                     /locus_tag="ACHJ48_001639"
     CDS             3116..4009
                     /gene="nadC"
                     /locus_tag="ACHJ48_001639"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO4707217.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4212..4652
                     /gene="ppdD"
                     /locus_tag="ACHJ48_001640"
     CDS             4212..4652
                     /gene="ppdD"
                     /locus_tag="ACHJ48_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO4707218.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4662..6047
                     /gene="gspE"
                     /locus_tag="ACHJ48_001641"
     CDS             4662..6047
                     /gene="gspE"
                     /locus_tag="ACHJ48_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO4707219.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6037..7239
                     /gene="hofC"
                     /locus_tag="ACHJ48_001642"
     CDS             6037..7239
                     /gene="hofC"
                     /locus_tag="ACHJ48_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO4707220.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQRAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGIMLTIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNIAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7274..8317)
                     /gene="guaC"
                     /locus_tag="ACHJ48_001643"
     CDS             complement(7274..8317)
                     /gene="guaC"
                     /locus_tag="ACHJ48_001643"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO4707221.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIIS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8473..8517)
                     /locus_tag="ACHJ48_001644"
     CDS             complement(8473..8517)
                     /locus_tag="ACHJ48_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707222.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8542..9162
                     /gene="coaE"
                     /locus_tag="ACHJ48_001645"
     CDS             8542..9162
                     /gene="coaE"
                     /locus_tag="ACHJ48_001645"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO4707223.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9162..9905
                     /gene="zapD"
                     /locus_tag="ACHJ48_001646"
     CDS             9162..9905
                     /gene="zapD"
                     /locus_tag="ACHJ48_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO4707224.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDCQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9915..10112
                     /gene="yacG"
                     /locus_tag="ACHJ48_001647"
     CDS             9915..10112
                     /gene="yacG"
                     /locus_tag="ACHJ48_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO4707225.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10203..10601)
                     /gene="mutT"
                     /locus_tag="ACHJ48_001648"
     CDS             complement(10203..10601)
                     /gene="mutT"
                     /locus_tag="ACHJ48_001648"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO4707226.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10661..13366)
                     /gene="secA"
                     /locus_tag="ACHJ48_001649"
     CDS             complement(10661..13366)
                     /gene="secA"
                     /locus_tag="ACHJ48_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO4707227.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13428..13940)
                     /gene="secM"
                     /locus_tag="ACHJ48_001650"
     CDS             complement(13428..13940)
                     /gene="secM"
                     /locus_tag="ACHJ48_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO4707228.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14171..15088)
                     /gene="lpxC"
                     /locus_tag="ACHJ48_001651"
     CDS             complement(14171..15088)
                     /gene="lpxC"
                     /locus_tag="ACHJ48_001651"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO4707229.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15189..16340)
                     /gene="ftsZ"
                     /locus_tag="ACHJ48_001652"
     CDS             complement(15189..16340)
                     /gene="ftsZ"
                     /locus_tag="ACHJ48_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO4707230.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16401..17663)
                     /gene="ftsA"
                     /locus_tag="ACHJ48_001653"
     CDS             complement(16401..17663)
                     /gene="ftsA"
                     /locus_tag="ACHJ48_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO4707231.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17660..18490)
                     /gene="ftsQ"
                     /locus_tag="ACHJ48_001654"
     CDS             complement(17660..18490)
                     /gene="ftsQ"
                     /locus_tag="ACHJ48_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO4707232.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18492..19412)
                     /gene="ddlB"
                     /locus_tag="ACHJ48_001655"
     CDS             complement(18492..19412)
                     /gene="ddlB"
                     /locus_tag="ACHJ48_001655"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4707233.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRVEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSVRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19405..20880)
                     /gene="murC"
                     /locus_tag="ACHJ48_001656"
     CDS             complement(19405..20880)
                     /gene="murC"
                     /locus_tag="ACHJ48_001656"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO4707234.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20934..22001)
                     /gene="murG"
                     /locus_tag="ACHJ48_001657"
     CDS             complement(20934..22001)
                     /gene="murG"
                     /locus_tag="ACHJ48_001657"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO4707235.1"
                     /translation="MSAQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21998..23242)
                     /gene="ftsW"
                     /locus_tag="ACHJ48_001658"
     CDS             complement(21998..23242)
                     /gene="ftsW"
                     /locus_tag="ACHJ48_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO4707236.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23242..24558)
                     /gene="murD"
                     /locus_tag="ACHJ48_001659"
     CDS             complement(23242..24558)
                     /gene="murD"
                     /locus_tag="ACHJ48_001659"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO4707237.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGVQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24561..25643)
                     /gene="mraY"
                     /locus_tag="ACHJ48_001660"
     CDS             complement(24561..25643)
                     /gene="mraY"
                     /locus_tag="ACHJ48_001660"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO4707238.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25637..26995)
                     /gene="murF"
                     /locus_tag="ACHJ48_001661"
     CDS             complement(25637..26995)
                     /gene="murF"
                     /locus_tag="ACHJ48_001661"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO4707239.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIVWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAIPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFAEKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26992..28479)
                     /gene="murE"
                     /locus_tag="ACHJ48_001662"
     CDS             complement(26992..28479)
                     /gene="murE"
                     /locus_tag="ACHJ48_001662"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO4707240.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQDATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEDRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28466..30232)
                     /gene="ftsI"
                     /locus_tag="ACHJ48_001663"
     CDS             complement(28466..30232)
                     /gene="ftsI"
                     /locus_tag="ACHJ48_001663"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO4707241.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30248..30613)
                     /gene="ftsL"
                     /locus_tag="ACHJ48_001664"
     CDS             complement(30248..30613)
                     /gene="ftsL"
                     /locus_tag="ACHJ48_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO4707242.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30610..31551)
                     /gene="rsmH"
                     /locus_tag="ACHJ48_001665"
     CDS             complement(30610..31551)
                     /gene="rsmH"
                     /locus_tag="ACHJ48_001665"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO4707243.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31553..32011)
                     /gene="mraZ"
                     /locus_tag="ACHJ48_001666"
     CDS             complement(31553..32011)
                     /gene="mraZ"
                     /locus_tag="ACHJ48_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO4707244.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32613..33617)
                     /gene="cra"
                     /locus_tag="ACHJ48_001667"
     CDS             complement(32613..33617)
                     /gene="cra"
                     /locus_tag="ACHJ48_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO4707245.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33699..33785)
                     /locus_tag="ACHJ48_001668"
     CDS             complement(33699..33785)
                     /locus_tag="ACHJ48_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO4707246.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33797..34288)
                     /gene="ilvN"
                     /locus_tag="ACHJ48_001669"
     CDS             complement(33797..34288)
                     /gene="ilvN"
                     /locus_tag="ACHJ48_001669"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4707247.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLYKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34291..36015)
                     /gene="ilvI"
                     /locus_tag="ACHJ48_001670"
     CDS             complement(34291..36015)
                     /gene="ilvI"
                     /locus_tag="ACHJ48_001670"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO4707248.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIG
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36333..37277)
                     /gene="leuO"
                     /locus_tag="ACHJ48_001671"
     CDS             complement(36333..37277)
                     /gene="leuO"
                     /locus_tag="ACHJ48_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO4707249.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9104 a        10456 c         9143 g         8869 t
ORIGIN      
        1 acaaacggtt tctttttaag caactcatct tcaaccatgc ataaagcggg tgcattcgct
       61 gccgcatacc attattcttg atctgacgga agtctttttg taactattca aacttctttg
      121 atgtaaacaa attaatacaa caaacggaat tgcaaactta cacacgcatc actgcgtaga
      181 tcaaaaaaac aatcaccgca cgaggtttca tgatggaagg tcaacagcac ggcgagcagc
      241 taaagcgcgg ccttaaaaac cgccatattc agcttatcgc gctgggtggc gcgataggga
      301 cagggttatt cctgggtagc gcctccgtaa tacagtccgc agggccaggg attatcctgg
      361 gttacgccat tgctggtttt atcgcctttc tgatcatgcg tcagctgggt gaaatggtgg
      421 tcgaagaacc tgtcgcaggc tcctttagcc actttgctta taaatactgg ggcagctttg
      481 ctggcttcgc ttctggctgg aactactggg tactgtacgt tttagttgcc atggcagagc
      541 tgactgctgt gggtaaatac attcagttct ggtatccgga aatcccaacc tgggtttctg
      601 ccgccgtgtt ctttgtggtg attaacgcca tcaacctgac caacgtaaca gtgtttggtg
      661 agatggagtt ctggtttgcc attatcaaag ttattgcggt agtagcgatg atcatcttcg
      721 gcggctggct gctgttcagt ggtaacggcg gtccgcaggc aagcgttagc aacctgtggg
      781 atcagggcgg tttcctgccg cacggcttca ccgggctggt gatgatgatg gcgattatca
      841 tgttctcgtt cggtggtctg gaactggtgg ggatcaccgc agcagaagct gataacccgg
      901 agcaaagtat cccgaaagca actaaccagg ttatctaccg catcctgatt ttctatattg
      961 gttcgttagc cgttctgctc tcactgatgc cgtggacccg cgttaccgcc gataccagtc
     1021 cgtttgtgct gatcttccac gagttaggcg atacctttgt ggcgaatgcg ctgaacatcg
     1081 tggtactgac tgcggcgctc tccgtgtaca acagctgcgt atattgcaac agccgtatgc
     1141 tgtttggtct ggcacaacag ggtaacgcgc caaaagcgct ggcgtctgtc gataaacgcg
     1201 gcgtaccggt taacaccatt ctggtgtctg cgctggttac agcattgtgc gtattgatta
     1261 actatcttgc tccggaatcc gcatttggcc tgttaatggc actggtggta tccgcactgg
     1321 tgatcaactg ggcgatgatc agtctggcgc atatgaagtt ccgtcgcgcc aagcaggaac
     1381 aaggcgtggt aactcgcttc cctgctctgc tttatccgct gggtaactgg atctgcctgc
     1441 tgtttatggc ggtggtactg gtgattatgc tgatgacccc aggaatggcg atttcggtat
     1501 acctgatccc ggtatggctg gtggtgttag gtatcggcta tctgtttaaa gagaaaaccg
     1561 caaaagccgt aaaagcacat taatctctct acgccctcac ccgtacaggg tgagggcaat
     1621 aatctttaca ccaacgcccc gtaaatcgtc agtagcgcaa tcaccaccac gaccacgaac
     1681 gaggttttct tcgccattga aaccgctgcc ttcggcgtct ccaccttatc gacatgcggt
     1741 tcacgcgcca gagagaactg cgccagacgc gttaatacct gatattgcga agtacggaaa
     1801 tcacccagcg aagcaaacca ggccggtaac gctttctcac catgaccgat caaggcatat
     1861 accacacctg caagacgaac cggcacccaa tccagtacat gaagcactgc atcaatgccg
     1921 gactgtaaac gatgatgcgg cgtctggtat cgcgccagcc agtattgcca tgcacgcaaa
     1981 aacgcatacc ccatcagcgt aacgggtccc caggttcccc ccacaatcag ccagaacagc
     2041 ggtgcaagat aaaaacgaaa gttaatccac agcaatgcat tttgcagctc acgcaaatac
     2101 tcacgttcgt cgcagcctgc cgggacgccg tgaatcatgg tgagttcgcc agccatcgtg
     2161 gcacgggcat ggctatcatt acgtgaagca gctgtcagat aagcatgata atgaagacga
     2221 actttacctg cgccaataca cagcaaacca atcagcagcc acaccagcag cgtgggaacg
     2281 ttgaacaata ctccctgcaa tgcgcgtaac agtaaaaaag tcacgcccat cgcaataatg
     2341 gtcatgccta acgtgcgccc gagagaaaaa tgtttcaccc gccgaaagaa cgcttcaaga
     2401 cgatgatcaa gctgccagtg ctcgcccaac ttaaacaggc gctcgaaaat taacaccagt
     2461 aaggttgtaa atagcgtcat gttgtctcct tgctgaccag cgcacgaaac cgtgcccaat
     2521 caaatgcagg accgggatcg gttttccgat ccggcgcaat atcacaatgg cccgtcatgt
     2581 tattagcgat atccggatag caatcaatca gtgcccgcgt aaccgccgca agctgttgat
     2641 actgcgcatc ggtatacgcc agcgtatcgg tgccttcaag ctcaatccca atagaaaaat
     2701 cattgcagcg ttcgcgcccc tgatactgag agactcccgc atgccatgca cgtttatcga
     2761 aaggaacata ctggactatt tcaccatcac ggcgaatcaa acagtgagcg gagacgcgca
     2821 aatgggcgat ctcagcaaag aaaggatgtg cctgcggatc aatagttcca gtgaataatg
     2881 cgtcgatcca cggaccgcca aactcgcctg gcggcaggct aatattgtgc accaccagca
     2941 gggtgggtgt ttcgtcatcc gggcggcaat cgtaatgtgg tgagggaacg cggcgcgcgc
     3001 caaccagcca cccctgttct aacaacatgc aggatctcct tatatgtggt gctaataccc
     3061 ggttcagagt agcatgtttc taccttatga ttcgttagct atctggagtt ttaacatgcc
     3121 gcctcgccgc tataaccctg acacccgacg tgacgagctg ctggaacgca ttaatctcga
     3181 tatccccggc gcggtggccc aggcgctgcg ggaagattta ggcggaacag tcgatgccaa
     3241 caatgacatt acggcaaaac ttttaccgga aaattctcgc tctcatgcca cggtgatcac
     3301 ccgcgagaat ggcgtatttt gcggcaaacg ctgggttgaa gaggtgttta ttcaactggc
     3361 aggcgacgat gtcaccataa tctggcatgt ggatgacggc gatgtcatca acgccaatca
     3421 acccttgttc gaacttgaag gcccatcccg cgtgctgtta acgggcgaac gcactgcgct
     3481 taattttgtg caaacccttt caggagttgc cagtaaggta cgccactatg tcgaattact
     3541 ggaaggcacc aacacgcagt tgttggatac gcgtaaaacc ttacccggtc tgcgttcagc
     3601 gctgaaatac gcggtacttt gcggcggcgg agcgaatcac cgtctggggc tttctgatgc
     3661 cttcctgatc aaagaaaacc atattattgc ctccggctca gtgcgccagg cggtcgaaaa
     3721 agcgtcctgg ctgcacccgg atgcgccagt agaagtcgaa gtagagaatc tggaagaact
     3781 tgatgaagcc ctgaaagcag gagccgatat catcatgctg gataacttcg aaacagaaca
     3841 gatgcgcgaa gccgtcaaac gcaccaacgg caaggcgcta ctggaagtgt ctggcaacgt
     3901 cactgacaaa acactgcgcg aatttgccga aacgggcgtg gactttatct ccgtcggtgc
     3961 gctaactaag cacgtacagg cactcgacct ttcaatgcgt tttcgctagt tgctgacaga
     4021 tttatccgct ccatagtggg cggataatct ccccttcccc aaccttcttc gtaacgcctc
     4081 gcaaatttct tgtctttcgc tgcaacgaaa taaaaaacct cggaagcagc tttcccgttt
     4141 cgttttttgc ccctcgcctg tgctgatggc gtttgccaaa gtagcaccaa ccaaatcaag
     4201 gagcgaaaca gatggacaag caacgcggtt ttacacttat cgaactgatg gtggttattg
     4261 gcatcattgc cattttaagc gccattggta ttcccgctta tcaaaactac ctgcgcaaag
     4321 ccgcactcac cgacatgcta caaacctttg tgccttaccg taccgccgta gagttgtgcg
     4381 cgctggaaca tggtggatta gatacctgcg acggtggcag caatggcatt ccctcgccta
     4441 ccaccacccg ctatgtttca gccatgagtg tggcaaaggg cgtggtgtcg ctgaccgggc
     4501 aagaaagtct caatgggcta agcgtcgtca tgacgccagg ttgggataac gcaaacggcg
     4561 tcaccggctg ggcgcgcaac tgcaatattc aaagtgacag cgcattgcag caagcctgcg
     4621 aagatgtctt ccgctttgat gacgccaact aaggagcggc aatgaatatt ccacagctca
     4681 cggccctgtg cctgcgttat cagggagtct tgctggatgc cagcgaagaa gtggttcatg
     4741 ttgcggtggt cgatgccccc tcacatgagt tgctggacgc attgcatttc gctaccacca
     4801 aacgtattga gatcacctgc tggacgcgcc aacaaatgga aggtcacgcc agtcgcacac
     4861 aacagacatt gcccgtagct gttcaggaga agcatcagcc caaagcagag ttgctgactc
     4921 gaacgttaca atctgcgctg gaacaacgcg cgtctgatat tcatatcgaa ccagcggaca
     4981 atgcctaccg catccgcttg cgtatcgacg gcgtattgca tcctttaccg gatgtttcac
     5041 cggatgccgg agtcgcatta accgccagat taaaagtgct gggaaacctg gatattgcgg
     5101 aacatcgcct gccgcaggac gggcaattca ctgtcgaact ggcaggaaac gccgtctcat
     5161 ttcgtattgc gaccttacca tgtcggggtg gtgaaaaggt ggtattaagg ttgttacagc
     5221 aggtgagcca ggcactggat gttaacacgc tgggaatgca gccgttacaa ctggcgggct
     5281 ttgctcatgc cttgcaacaa ccacagggac tggtgctggt aactggccct accggcagcg
     5341 gcaaaacggt cacgctttat agtgccctgc aaaagctgaa taccgctgac attaatattt
     5401 gtagcgtcga agatccggtt gagatcccca tagccggact aaaccagacg caaatccatc
     5461 cgcgtgccgg actcaccttt cagggcgttt tgcgtgcgtt attgcgccag gatcctgacg
     5521 tcatcatgat cggagagatc cgcgatggcg aaacggcaga aattgccatt aaagccgcgc
     5581 aaaccggtca cctggtgttg tctaccctac acactaattc cacctgcgaa acgctggtac
     5641 gtttacagca aatgggggtc gcccgctgga tgctatcatc ggcgcttacg ctggtaatag
     5701 cccagcgtct ggtacgcaaa ctttgcccac attgtcgccg gcagcaaggg gagcccatcc
     5761 acattccagt caatgtatgg ccgtcgccgc tgccccactg gcaagcaccc ggttgtgtac
     5821 attgctacca cggtttttat ggtcgcactg ccttatttga agttctgccc ataacaccgg
     5881 tcatacgtca gcttatttcc gctaataccg acgttgaatc gctggaaacg cacgcccgac
     5941 aggcgggtat gcgaacgctt tttgaaaacg gctgcctggc cgtggagcaa ggcttaacca
     6001 cctttgaaga gttaatccgc gtattgggga tgccgcatgg cgagtaagca actctggcgc
     6061 tggcatggca taaccggcga cggcaatgcg caagatggga tgctatgggc agagagccgt
     6121 actttactgc ttatggcact acagcaacag atggttaccc cactaagcct gaagcgaatt
     6181 gctatcaatt ctgcgcagtg gcgaggagat aaaagcgcgg aagtcattca tcaactggcg
     6241 acgctactta aagccgggtt aacgctttct gaagggctgg cactgctggc ggaacagcat
     6301 cccagtaagc aatggcaagc gttgctgcaa tctctggcac acgatctcga acagggcatt
     6361 gccttttcca atgccttatt accctggtca gaggtatttc cgccgctcta tcaggcgatg
     6421 atccgcacgg gtgaactgac cggtaagctg gatgaatgct gctttgaact ggcgcgtcag
     6481 caaagagccc agcgtcagtt gaccgacaaa gtaaaatcag cgttacgtta tcccatcatc
     6541 attttagcga tggcaatcat ggtggttgtg gcaatgctgc attttgttct gccggagttt
     6601 gccgctatct ataagacctt caacacccca ctaccggcac taacgcaggg gatcatgacg
     6661 ctggcagact ttagtggcga atggggctgg ctgctggtgt tgttcggcat tatgctgacg
     6721 atagccaata agttgctgat gcgccgcccg acctggctta tcgcgcggca gaaattgctg
     6781 ttacgcatcc cgattatggg gtcactgatg cggggacaaa aactcacgca gatctttacg
     6841 attctggcgc tgactcaaag tgcaggcatt acttttttac agggcgtaga gagcgtcaga
     6901 gaaacaatgc gctgcccgta ctgggtgcaa cttctgacac aaatccagca cgatatcagt
     6961 aacggtcatc ccatctggct ggcgttaaaa aatgccggtg agtttagccc gctctgtttg
     7021 caattagtga gaacgggaga ggcctccggc tcgctggatc tcatgttaga caacatcgcc
     7081 catcatcatc gggataacac aatggcgctg gcggataacc tagcagcctt actggaaccg
     7141 gcgttgctga tcataacggg aggaattatc ggtacgctgg tggtggcgat gtatctgcca
     7201 attttccatt taggcgatgc gatgagtggg atgggataac gctggcgtgt tgctccacac
     7261 cagcgttgga agattacagg ttgttgaaga tgcggttttc ttgttcctgc acacggataa
     7321 atgtggtgcg cttggtcagt tctttcaggc gtgaagcccc aacgtatgta caagctgaac
     7381 gcaggccgcc caaaatatca cgcgcggtat tttcaaccgg gcctcgcagc ggcagcttaa
     7441 cggttttacc ttctgctgcg cgatattcag caacgccgcc aacgtgacgt ttcatcgcag
     7501 actcggagct catgccgtag aacagcataa atttctcgcc gttctcctca acgatgcgac
     7561 cgccgctctc ttcgtggccc gccagcatgc cgccaagcat gacgaaatcg gcaccgccac
     7621 caaaggcttt cgccacatcg cccggcgtgg tgcagccacc atcgctgatg atcattccgc
     7681 ccagaccgtg cgcagcatcg gcacattcga ttaccgcaga aagttgcgga taaccgacgc
     7741 ctgttttgac gcgagttgta caaacagaac ctgggccaat gccaacttta acgatatcgg
     7801 cacctgagag gataagctcc tcacacattt caccagtcac tacgttacca gcacaaatgg
     7861 ttttggtcgg ccacgcttca cgcgctttcg caacgaactg cacgaagtgt tcggaataac
     7921 cattcgccac gtcaatacaa acgaagttta atgccgggtt caggtcgaga atctgtttag
     7981 ttttttcgaa atccgcatca gacgtaccgg tagaaaccat cacatgtttc agcacatcag
     8041 cggaagaatt gttgataaac gcttgccact cttcgacaga atagtgttta tgcacagcag
     8101 tcaaaatatc aaaagaagcc agcgcagagg ccatagaaaa tgtgcctacg gtgtccatat
     8161 ttgcggcgat aatcggcacg ccggaccagc tctgacctga atgtttgaag gtgaattgac
     8221 gttccagttc aacatcggaa cggcttttaa gagtggagcg tttagggcgg atgagaacgt
     8281 ctttaaaacc taacttcaga tcttcttcaa tacgcatgtg cggattcctg gggttaatgg
     8341 ctataagggt aagagcgcaa ctccagtgac gctatcatac gcactaatca atgcggcgca
     8401 agactgcgaa attgtctttt tttacgctac aatcccataa atttacctgg taaatagtga
     8461 gcgaacgttt catcaatttc gctttcgtgc gctcattttt aacgttttga taatcatgat
     8521 gaattcccgg gaatataaat tatgaggtat atagttgcct taacgggagg cattggcagt
     8581 ggcaagagta ccgttgccaa tgcgtttgct gatctcggaa ttaacgtcat tgatgccgat
     8641 attattgcgc gtcaggtggt tgaaccaggt gcacctgcgc tacatgccat tgctgatcac
     8701 tttggcgcta atatgattgc tgctgatgga acattgcagc gccgggcctt gcgcgagcgg
     8761 atcttcgcca acccggaaga gaaaaactgg cttaacgccc tgctgcatcc gctgattcag
     8821 caagagacgc aacaccagat ccagcaagct acttccccct atgtactgtg ggttgtgcca
     8881 ttgctggtag aaaactcact gtataaaaaa gcgaatcgag tgctggtggt ggatgtcagc
     8941 ccagaaacgc aacttaagcg caccatgcag cgcgatgatg taactcgcga gcatgtcgaa
     9001 caaatccttg ctgctcaggc aacgcgcgaa gcccgccttg ccgtggcaga tgacgtcatt
     9061 gataataacg gcgcaccgga tgctatcgca tcggatgttg cccgcctgca cgcacactat
     9121 ttgcagcttg cgtcgcagtt tgtctcacag gaaaaaccgt aatgcagacc caggtccttt
     9181 ttgaacatcc actaaatgaa aaaatgcgta catggctgcg cattgagttt ttgattcagc
     9241 aactcaccgt taatttaccc atcgttgacc acgctggcgc gctgcatttc ttccgtaatg
     9301 ttagtgaatt actggatgtt ttcgagcgcg gcgaagtccg cactgagctg ttgaaagaac
     9361 ttgaccggca gcaacgtaaa ctccagacct ggattggcgt gcctggcgtg gaccagagcc
     9421 gtattgaagc attaattcag cagttaaaag cggcggggag cgtattaatt tccgcgccgc
     9481 gtatcgggca atttctgcgt gaagatcgtt tgattgctct ggtgcgtcag cgactgagca
     9541 tcccaggcgg ctgttgcagc tttgatttac ctacattgca catttggctg catctacccc
     9601 aggcgcagcg cgactgccag gtagaaacct ggattgccag cctgaacccg ctcacccagg
     9661 cacttaccat ggtgctggat ttaattcgcc agtcggcccc cttccgtaaa caaaccagcc
     9721 tgaatggttt ttatcaggat aacggtggcg atgccgactt gctgcgcctg aatctgtcgc
     9781 tcgattcaca gctttatccg caaatttccg gacataagag ccgttttgcc attcgtttta
     9841 tgccgctgga cactgaaaac ggacaggtac cggaacgtct ggatttcgaa ctggcctgtt
     9901 gctaaggagt aaaaatgtca gaaactatta cggtgaattg cccaacctgc gggaaaacgg
     9961 tggtgtgggg tgaaatcagc ccgtttcggc cattttgctc caaacgttgt cagctgatcg
    10021 acctcggaga atgggctgct gaagaaaaac gaatccccag cagtggcgat ctttccgaaa
    10081 gcgatgactg gagcgaagag ccaaagcagt gacatttgtc tgatgccgca cgtaggcctg
    10141 ataagacgcg gacagcgtcg catcaggcat cttgtgcaaa tgtcggatgc ggcgtgaacg
    10201 ccttatccga catacacacg cttaagcttc gcaattaccg gttcattggc tggcggaaaa
    10261 tcatcggcat taagaccgac tagcgacatc cacttaccgg gttgcccttc tttaccccac
    10321 ggctcccctt cccagctttc gaccagccag aaccacagag ttatatgcct gtccgggaat
    10381 tcatattcca gtttttcaaa tagcgaaaaa tgttgtgggg taatcccgac ttcttcctga
    10441 agttcacgca ccaccgcctg ttccggcgtt tcacccattt caattttacc gccgggaaac
    10501 tccagtttat tcgccatgtg cgcatctgct gcgcgacgcg ttataaagat ttcattgttc
    10561 tcgttgcgaa taatacctac cgcaatttgc agctttttca ttgtcttaaa cctataaaaa
    10621 aggcgcagaa tcctgcgcct tttacttcaa cagttagctt ttattgcagg cggccatggc
    10681 actgcttgta ttttttacca gaaccgcacg ggcaaggatc gttacgtcct actttgcgct
    10741 caccggtttg cgccgccagt gcagctgcgg ctgcagagtc gtcatcctga tggctaagct
    10801 gctgcatttg cgctaaacgc tcggcttcca tacgacgctg ttgttccagc tcctcaacct
    10861 cttcaggcat acgtacctga actttgctca gcgtactgat aacttcatat ttcaacgact
    10921 ccagcatcgc tgcaaacatg gagaacgatt cacgtttgta ttcctgcttc ggatctttct
    10981 gtgcatagcc acgcaggtgg ataccctgac gcagatagtc catcgctgcc aggtgctctt
    11041 tccacaggga gtcaagcgtt tgcagcatga cgcctttctc gaagtgacgc atcatctcag
    11101 caccaaccac ttcttcttta cgctgataca cttcgatgga ctgcgccaga atgcgctcac
    11161 gcagcgtctc ttcatgcagt tctggttctt tatccagcca ctcagcaatt ggtaaatcga
    11221 ggtcgaaatc gttcttcaga cgttcctgca gccccggaat atcccacatt tcttccagcg
    11281 actgtggcgg aatgtaggca tcaatggtcg ctttgaacac atcttcacga atgctgttaa
    11341 tggtttcgct cacatcgctg acatccagca attcgttacg ctgggagtaa atggcgcgac
    11401 gctgatcgtt agccacgtca tcatattcca gcagttgctt acgaatgtcg aagttacgac
    11461 tttcaacttt acgctgggcg ttggcaatcg ctttagtcac ccacgggtgt tcaatggctt
    11521 cgcctggctt catacccagt ttacgcatca tgccggatac tcggtcggaa gcaaaaatac
    11581 gcatcagcgc atcttccatc gacaggtaga aacgggaaga accagcatcc ccctgacgac
    11641 cagaacgacc gcgcaactgg ttatcgatac gacgggattc gtgacgctcg gtaccgatga
    11701 tatgcaggcc acctgctgcc agtaccgcat cgtgacgtac ctgccagtcg gctttaattt
    11761 tttcaatttg ctctgcggtc ggattttcca gcgcggcaac ttctgcctgc cagctaccac
    11821 cgagcacaat atctgtacca cgacccgcca tgttggtcgc gatagtcacc gcagccggat
    11881 aacctgcctg agcaacaatc gccgcttcgt tggcgtggaa tttggcgttc aggacattgt
    11941 gcttaatacc ggctttggtc agttcgtttg acaccagctc cgatttttcg atggagatag
    12001 tacccaccag caccggctgg cctttcgcag tacgttcttt gatatcttca atgatcgcct
    12061 gaattttttc cgcttcagtc atgtagacca ggtccggcag atctttacga atcattggac
    12121 ggttggtcgg aacaacgacg gtatccagtt tgtagatgga gctaaattcg aaagcttcgg
    12181 tatcagcagt accggtcatc cccgccagtt tttcatacag acggaagtag ttctggaagg
    12241 tgatcgaagc cagcgtttgg ttttcgttct ggatctgcac accttctttc gcttccacag
    12301 cctggtgcag accatcggac cagcgacggc cctgcatggt acgaccggtg tgttcgtcaa
    12361 cgatgataac ttcaccatct ttaacgatgt agtcgacgtc acgggtaaac agcgcatgag
    12421 cgcgcagcgc cgccgttacg tggtgcatca gcatgatgtt ggccggagag tacagagact
    12481 ccccttcatc catgatgccc tctttcacca gcagttcttc aatcagcacc agaccacgtt
    12541 cggtcaggtt cacctggcga gatttttcgt ccaccgagaa gtggccttcg ccctggaagg
    12601 tttcggagtc ttctttttcc tgacggatca ggtgcggaat aattttattc acgcgtttat
    12661 acatttccga gctgtcttct gccgggccgg aaatgatcag cggtgtacgc gcttcatcga
    12721 tcaggatgga gtccacttcg tccaccagcg catagtgcag tttacgctgt acacgttctt
    12781 cagggctgaa cgccatattg tcgcgcaggt agtcaaagcc gtattcgttg ttcgtaccgt
    12841 aagtgatgtc agctgcgtaa gcttcgcgct ttgccggtgc tggcatgccc ggcaggttga
    12901 taccgacagt caggccaagg aattcaaaca gcggacggtt gttttcggcg tcacgttgcg
    12961 ccaggtagtc gttgacggta actacatgca cgcctttacc ggttagtgcg ttcagataag
    13021 caggcagcgt tgcggtcagg gttttacctt caccggtacg catttcggcg atgcagcgtt
    13081 cgttaagaac cataccgccg agtaactgaa cgtcgaagtg acgcatacca aagacgcgct
    13141 tacttgcctc acgtaccacg gcgaaagctt ccgggatcag attttccagc acttcgcctt
    13201 tttccagacg tgcacgaaac tctgcggttt tccctttcag ttcttcgtcg gagagttttt
    13261 ccatctccgg ttccatggca ttgatgatgt tgaccacttt gcgcatccgg cgcagggtgc
    13321 gatcgttacg actaccgaaa actttagtta ataatttgat tagcataata aaatctcaaa
    13381 cgccccgcgt tgcggagtta ataaaatgaa gtaaaggttt attgttgtta gctgaggcgt
    13441 tgagggccag cacggatgcc ttgcgcctgg cttatccaga cgggcgtgct gaattttgct
    13501 tgtggggtaa aatgcgcata atcaatgcga taaccctttt cagacggcgt gccttcctgg
    13561 gtcagcagcg cgctgagcgt atccagtaat gcaagatgtt gcgcctgaag aggcaaagat
    13621 tcttcagcaa cgggcagcgt ttgcggtgcc attgcgaagg aaagatgacg gattaccgtg
    13681 cgaatggcat gttgatgcca gtaatctacg gaatagttcg aattcgggcg gcgcgtgttc
    13741 gcttccagca aggccaattg accaaagtta actttggctg aaggctcgtg gttgcgggtt
    13801 gtcgcttttg cgggcgcgtt tggttcggcg gcgttgctga gcgcaggcaa acctaaactc
    13861 gccgcaacca tccctaataa gagatgcggc cagaagtagc gtttaccaaa ctgtcgccag
    13921 cgcgtcagta ttccactcac gttattgcca tcccgatcgt attcaaacgt atttagcgca
    13981 caaatcttat cattaaagcc tgaagtccac agcacgaatg aagataagtt gtccgtaaaa
    14041 gtgcttaaga aaacagcgtt cgcaccgttt tttatagctc ttataaaatt caaaaaaaaa
    14101 cgactggttc acctggccgg agagagtgcc agatttgcca gtcgaatttt atacgacagt
    14161 ataaatgtcg ttatgccagt acggctgaag gcgctttgaa ggccaacggc agttctgcgt
    14221 cgtcctggaa ggtcacatat tcccaggctt cctgtttcgc caggacagcc tgcagcagtt
    14281 tgttattcag tgcatgaccg gatttataag cggtaaatgc accaataata ttgtgaccac
    14341 acatgaacaa gtcaccgatc gcatcgagca ttttgtgacg cacaaattcg tcttcaaaac
    14401 gcaggccgtc ttcgttcagt acgcgataat cgtcaacaac gatggcacaa tcgaagctgc
    14461 cgcccaggca caaaccacgg gactgcagat attcgatatc acgcatgaaa ccgaacgtac
    14521 gcgcacggct gatctggcgc ataaacgcat cagcggagaa gttcatcgca tagcgctggt
    14581 tgctggaatc aatagccgga tggttaaaat cgatggtgaa atccagcgaa aaaccattgt
    14641 acggcttaaa ttcagcccac ttatcgccat cttcgacacg aacagtctct ttgatgcgaa
    14701 caaatttttt ggcgcagttc aactcgtcga taccggcgtc aagcagcagg tatacaaacg
    14761 gagcggcgct gccgtccatg atcgggattt ccggcgcgtt aacttcgata acaatgttat
    14821 cgatgcccaa gcccgcgaga gcagcattga ggtgctctac ggttgaaatc cgtacatcat
    14881 gctcgttgac cagacacgta cagagcatgg tatcacgcac agatttggca tcggccggga
    14941 aatctaccgg tggattcaag tcggtgcgac gatagatgac cccggtgttg gccggcgcag
    15001 ggcgtaacgt cagggtgact ttcttgccgg tatgtaaacc gacacccgtc gcctgaacga
    15061 tacgtttaag tgtcctttgt ttgatcatcg tattatctcg ccaaattacc tatccaaccg
    15121 aagtgtacta tacattcggc gggccagttt agcacaaaga gcctcgaaac ccaaattcca
    15181 gtcaattctt aatcagcttg cttacgcagg aatgctggga tatccagata atccggctct
    15241 ttcgcagttt gcggcgcatt gtcattcacg actttagcaa ccggcttctg ctcctgggtc
    15301 agcggagcca tcccatgctg ctggtagcga tccatcactg gctgctgaac ctgcttattg
    15361 gtcaccagag tgatttcagg acgtttgtcc atgccgatac ctgtcgcaac aacggttacg
    15421 cgcagctcgt cattcatatc cgggtcaaga gaagtaccga taaccacagt cgcgttgtcg
    15481 gaagcaaatg cacggatggt gttacctacc gtttcgaact catccagacg caggtcgaag
    15541 cccgctgtga tgttaaccag cacgccgcgt gcgccagaca ggtcgatatc ttccagcagc
    15601 ggagaagaga tagccatttc agcagcttct tccgcacggt cttcaccgct cgccacgcca
    15661 gaacccatca ttgcgtagcc catttcagac attacggtgc gtacgtctgc aaagtccacg
    15721 ttcatcaaac ccggacgagt aatcagttcg gcgatgcctt gcacagcgcc tttgagtaca
    15781 tcgttcgctg cgccaaacgc atccagcagg gagataccgc ggcccagaac tttcagcagt
    15841 ttgtcgttcg ggatagtgat cagagagtcc acatgcttgg acagttcagt gatcccctgc
    15901 tccgcgaatg ccatacgctt cttgccttca aagttgaagg gcttagtgac gacagcaacg
    15961 gtcaggatac ccaaatcttt tgccacttca gcgacgactg gtgctgcacc tgtaccggta
    16021 ccaccaccca tacccgcagc aataaagacc atgtctgcac cttccagcgc cgcacgcagt
    16081 gcatcgcgat cctcatctgc cgcattgcgg ccaacttctg gattagcgcc agcgcccagt
    16141 cctttggtga taccgctacc gatttgaatc gtctgtccaa ccgctgtttt acgcagcgct
    16201 tgtgcatcgg tatttaccgc gaagaattca acaccttcaa tgcgctcgcg caccatgtgt
    16261 tcaacagcat taccgccgcc gccgccgacg ccgatgactt taatcaccgc gtcattggta
    16321 agttccattg gttcaaacat agtttctctc cgatttgtgc ctgtcgcctg aggccgtaat
    16381 catcgtcggc ctcataaaaa ttaaaactct tttcgcagcc aactattgag tcgcttgatc
    16441 cacgagccaa ctgatgctgt aacacgtttt tctacttcag cttcaccgtt aagatgtgac
    16501 tctttcccat agtgaagcaa tcccaccgcc gtcgaataat acggctcctg agcataatcc
    16561 gttaaaccgg taatgttcag cggcgcgccg atacgcactt gcgtatgaaa cacgcgctga
    16621 gcacaggctg caagaccttc gatctgcgct gcgccaccgg ttaatacaat gcctgccgcc
    16681 aggtgatgtt taaccccttg ttggcgaagc ttttcctgca actgcaatat ctcttcgttg
    16741 accaggttga gcagctcggt atagcgcggc tcgatcacct ctgccagggt ctgacgttgc
    16801 agactccgcg gcggacgacc gcctacgctc ggcacttcca cgctctcatc ttttccaacg
    16861 atggaaccca gcgcacaacc gtggcgaact ttaatcgctt cggcgtcgct tggcggcgtg
    16921 ccaaaggcgt aagcgatatc actggtcacg acattgccag cataaggaat taccttagtg
    16981 tggcgcaatg ccccaccggt ataaacggcg atatccattg taccaccacc gatatcgacg
    17041 acgcagacac ccagttcacg ttcatcttcc gtcaataccg aataacttga tgccagaccg
    17101 gcaaatatca gttggtcaac tttcagccca caacgttcaa ccgctttgac gatgtttttc
    17161 gccatatcgt tgtggcatgt gatcaggtgc acttttgcct gcatccgcac gcccgaaagc
    17221 cctaccggat tcttgatccc ttcctgatag tcaatcgcat actcttgcgg gatcacatgc
    17281 agcacacgat gctcatcgcg tacacgcacc gatttcgcgg tatggacgac gttttccaca
    17341 tcttcttgcg ttacttcttc ttcagaaata ggcaccatac caatttcatt ctggcagctg
    17401 atgtgcttac cagaaagcgc cagatatacc gaagagatct gacaatctgc catcaattct
    17461 gcctggtcaa tggcacgttg tacgcacttg accacggatt cgaggtcgtt caccccgcct
    17521 ttatccatac cacgcgacgg gcagctgccc acgccaatga tattgaccat accgtcgggc
    17581 agaacttccc ctactaaagc ggcaaccttc gcggtaccaa tctccagtcc tactaccagt
    17641 tttctgtccg tcgccttgat cattgttgtt ctgcctgtgc ctgattttgt tgctgagtag
    17701 attcctctgg cggcaagggc gcccagccta ctgccgctcc agagtcataa cgcaaatcaa
    17761 cgtagctaat ccgtttgcca tcggtttgcg cctgctgctg tagaaccgga taaagttcta
    17821 caaagcgagc caaacgtttc atcgtatcgc cccggccaag attgagctta atatcgttat
    17881 tcagcgtcaa ctgccaggaa cgccgcgcgg tcatcgccgc ttccttcaga gtaaatctgt
    17941 cctttgccag catctgcccc atttcgcgat agccctgcaa cacttcattg gcgctgcctt
    18001 ccgggccata cagcattgga agcacctgct tgctggtgcg atctggcggc acgctgaagg
    18061 tatttccttc cgcgtctacc atgtgttgat cattccaccg cgcaatcggc acatattcaa
    18121 ccagatgaat cttcaattca tcaggccact gctttctgac gctcacctgc ttaatccacg
    18181 gcagacgttg ttctatttgc gtctggatga tgttgacatc ctgggtcata aaggtacccg
    18241 gctcacccaa tgccaggatc gactgccgga tatcgtcatt acgcgtgtaa tggcgttcac
    18301 cggtcaacac cagctttgag agcggcaggc gttgcgcatc ttccatccag cccaacacga
    18361 cccagccgct caccaacact gtcgttaaaa cggtcagcag gaaaaggatc cccgccagac
    18421 gcgttccatt attgcggcga gaagaaacct cttcttcgct gtttcgcgtg ttcagagcag
    18481 cctgcgacat attagtccgc cagttccaga attcgtacta ccaactgcga gaagctcata
    18541 cctgcctgac gtgccgccat cggcaccagg ctgtggctgg tcatacccgg tgaggtattg
    18601 gcttccagca gataaaactg tccatcgctg tccagcataa cgtcaatacg tccccatcct
    18661 ttgcaaccta acgtcgtcca tgctttcagc actaatgcct gcaaattggc ctcttgtgac
    18721 gcttccagac ctgcggggca gaaatactgt gtctcatcag agagatactt cgcctcataa
    18781 tcatagaagg ttccggacgg ttgaatacgt actgacggta aaatttcttc accgagtatc
    18841 gcaaccgtga actccggccc acttagccat ttttcaatca atacttcttc atcgtgctga
    18901 aatgccaatc ttaatgcatc ttgtagagca ttttctgcta ctacttttga cattcccaca
    18961 ctggaacctt cgcggctcgg cttaacgata accggcaaac ccagagcaga aatttctgct
    19021 aactgcttat cactcaggcc tttttcaaac tctacgcggg ttaacgctac ccacggcgcg
    19081 accggtaaac cggcaccttg ccacagaagt ttgctgcgta gtttatccat tgaaagcgca
    19141 gatgccatca ctccgctgcc ggtataaggc aagcccatca gctcgagcat cccctgcagc
    19201 gtaccatctt caccgccgcg gccgtgtagc gcgataaaca ctttctgaaa gcccatcgac
    19261 ttcagttgcg tcacgtcaac ttctttcggg tcgacaggat atgcgtcaat accgccttca
    19321 cgcagcccgg ctaacaccgc tgcgccagaa ttcagagaaa cttcccgctc agcggaagtc
    19381 ccgcccaaca ggaccgcgat tttatcagtc atgttgttct tcctccggag tttgcggctt
    19441 cagtttgatt tcagctaaag aacgggcaat ttttccgata ttaccagccc cctgaacgag
    19501 aatcaggtcg ttaccggtta ataccggtgc cagcatctcg gctacctgcg cagggtccgg
    19561 caccagaatg ggatctattt tcccacgtcc acgaattgtg cgacacagcg aacggctgtc
    19621 cgcacccgga attggcgctt cgccagccgg atacacttcc agcatcaaca gggtatcaac
    19681 ctgcgtcagc acattggcga aatcatcata caggtcgcgt gtacgggtaa aacggtgcgg
    19741 ctgaaacagc attaccaggt ttttatccgg ccagcctgcg cgcgccgctt taatggtggc
    19801 gtccacttcc gtcgggtggt ggccgtagtc atcgaccagc attgccgtac cgcttttacc
    19861 attcactggc tccagcggga attcaccgag gaaatcaaaa cggcgaccag tcccctggaa
    19921 gctctccagc gcacgcaaaa tagcctcgtc gtcaatgccc tcttccgtag caaccgcaac
    19981 cgcagctgcg gcgttcagcg cgttatgacg gcctggcgca ttcagggtga cgcgcatcgg
    20041 ctctttgtcc tggcgcagca gcgtaaagtg cccctgcggg ccaatctgct gataatcttc
    20101 tacacgcacg tcggcatctt cgctgaagcc gtaagttgtg gtctgacgtc ccactcgcgg
    20161 taacaattcg cggatcaccg gatcatcaac acacatcacc gcacgaccgt aaaacggcag
    20221 gttgtgcaga aaattaataa aagtctgttt taaattctca aagtcgccct ggtaggtatc
    20281 catgtggtcg gcttcgatat tggtgacaat cgccaccatc ggttgcagat gcaggaacga
    20341 tgcatcactc tcatctgctt cggcaatcag gtaccgacca tgccccaaac gcgcatgaac
    20401 ccccgccgct tttaccagcc cgccgttaac gaaggttggg tcgagccccg cttctgcgta
    20461 gatgctggag accatcgcgg tagttgtcgt tttgccgtgc gttcctgcaa tggcgatgcc
    20521 atgacgaaaa cgcattaact cagccagcat ttcggcacga cggatcaccg gaatacgcgc
    20581 ttcatgagct gcgacaattt ccgggttatc ggcagaaatg gcgctggaaa caacgaccac
    20641 gctggcatca cgtacgtttt ccgggcgatg gttgaaataa atcgtcgctc ccagattcat
    20701 taactgctgc gtgaccggat ttggcgctaa atcggaacca ctgatctgat aaccttcatt
    20761 ggccagaact tcggcaatac cgcccatacc ggcaccaccg atgccgacaa aatgtatgtg
    20821 ccgaacgcga cgcatttcgg gcacgatgga acgcagtttt gccaattgtt gtgtattcat
    20881 tctttacgcc attaacttct taaattcata cgatgcaaaa ggcatcgcta caattacgcc
    20941 cgggcagccc gactcacttc atttgccact cgctcggtgg catccggaat ggatgcagcg
    21001 cgggcgcgtt ctgccatggt taataaggtt tctcgcgacc acccggccag ggtgttggcg
    21061 acagcatcca cgctaagctg tggctgctcg ataattttgg ctgcgcccgc tttttccagc
    21121 ggtagcgcat tccagtattg ctggcggtct ttatgttgaa acggcacaaa caacgccggt
    21181 agtcctgccg cggcgatttc actcaccgtt aaagcaccgg agcggcaaac gacgacatcc
    21241 gcccacgcat acgccgccgc catatcatca ataaattcag tcactttatg ctgcggttgc
    21301 cccgcttcgg catacgcctg ttcaacggat tgttgcgaac ctttgccgct ctgatgccag
    21361 atagtgactg aatcacccag tttcgcagca acctgcggca ttgtctggtt aagaatgcgt
    21421 gcgccctggg aaccacccac taccagcaca cgaaccggac cttcacgtcc agccaaacgt
    21481 tgctgcggca acggtagcgc caacacatcg gtacgcaccg ggttacccac cacttccgca
    21541 ttggggaaag cacctggaaa cgcctgcatc actttggtgg caatcttcgc cagccattta
    21601 ttggttaagc ccgcaatacc gttttgttca tgaagtacaa ccggaatgcc taacgaccac
    21661 gcggccagac caccaggacc tgatacgtag ccgcccatac cgagcaccac gtcaggtttg
    21721 tacgctttca taatcgcccg cgcctgacgc caggcattga agatacgcag cggcgcagct
    21781 atcagtgctt ttataccttt tccacgcaga ccagagatac gaatgaaatc aatttcgatg
    21841 ccatgttttg gcactaagtc cgcttccata cggtcggcag tccccagcca gcgaacttgc
    21901 caaccctgag ccattagatg gtgcgcaacc gccagtcccg ggaatacatg tccaccggtt
    21961 ccgcctgcca tcaccattaa tcgctttcct tgagcactca tcgtgaacct cgtacaaacg
    22021 cctgcgcttt ctccagacgc gtttcataat caatacgcaa cagcatcatg atggctgtcg
    22081 acataatcag taagctcgaa ccaccgtaac tgatcagcgg caatgtcaga cctttggtcg
    22141 gtaacatccc cgccgccgcg cctacgttaa ccagcgcctg gaagctaaac cagatgccaa
    22201 tagaacaggc gagaaaaccg gaaaaacggt ggtcaatttc taatgcttta cggccaatcg
    22261 acatcgcgcg aaaagcgacg aagaatacca ttaaaagtgc cagcaccaca ccgacatacc
    22321 ccagttcttc gccgataatg gcgaaaataa agtcagtgtg cgcttccggc agatactcca
    22381 gtttttgtac agagttacct aaaccttgtc cccacagttc gccgcgacca aacgccatca
    22441 gcgattgcgt taactgatag ccgctgccaa agggatcttc ccacgggttc cagaatgcgg
    22501 taacacggcg gatacggtac ggttcggcga gtatcagcaa cacaaccgct gaaatgccca
    22561 taccgataat ggcaatgaac tgccacaatt tcgctcccgc caggaacaac atcgccagcg
    22621 tagtcacaaa caacaccacc accgtaccaa ggtctggctg tgccagcagt aacactgcca
    22681 acaccagaat cacgcccatc ggtttcagga agccgcgcag gttattacgt acttcgtcac
    22741 ctttacgcac cagatagttg gcgatatagc aaaacagcga cagttttgtc agctcagcag
    22801 gctggatacg cagcaaaccg agatcgatcc aacgcgatgc ccctttaacc gagctcccca
    22861 ctaccagcac gatcatcagc aggatgatag atccgagcag catcgtggca ctgtagcgtt
    22921 gccagaactc catcggcaga cgcagcgtaa tgatcgccag aataaacgcc aaaatcagat
    22981 agacaccatc acgcttcgcg aagaagaacg gatcgttggt taagcgttgc cctatgggca
    23041 ttgacgccga ggtcaccatg ataaagccaa tcgccgcgag gccgaaggtc agccacagta
    23101 aggtgcgatc gtacatgatc aggctgtcgg tatctttttc ccgcgagccc atcacccagc
    23161 cctttagcgc cgtggagatc cagaccagga tactgaatcc tggcaggcgc ggcattttca
    23221 ggcgagggag agataaacgc atcaacctaa ctccttcgcc agacgggcaa actcattgcc
    23281 tcgttgttca aagttcttga actgatcaag gctggcacag gccggggaga gtagaaccat
    23341 atcgcccggc tgaacacgcg gagccagcaa gcgcatcgcc tgttccatag tttcggtttg
    23401 ttctgccact tccgggcgta gcgccgccag ctgcacgccg tcacgaccga aacaatacag
    23461 acgtacgtta tcgccattca ggtaacgcgc cagtgggcta aagtccgccg atttaccatc
    23521 gccgcccagc aacaaatgca gcgtgccgtc tacgtgcagg ccattcagcg ccgcttccgt
    23581 actgccgacg ttggtcgctt tcgaatcgtt aatccaacgt acgccgttat gctccagcac
    23641 aacttcaaag cgatgcggca gaccagtgaa tgtggttaac gctttcaggc tgctggcacg
    23701 cggtaaccct gcggcatctg ccagcgccag cgccgccagc gcattggtgt agttatgctg
    23761 cccggaaagt ttcatttctt tcacgttcag cactttctcg cctttcaccc gcagccaggt
    23821 ttcgccctgc tgatgattca ggtgatagtc acccatgttg acgccaaagc tgacgcagcg
    23881 ttcatccgca ccgcgaatcg gcattgttaa ggcatcatca gcattgacga cacaaacttt
    23941 cgcattttcg taaatacgca attttgccgc acggtattgc tgtaaaccaa acggatagcg
    24001 atccatatga tcttcagtca cgttcagaat ggtcgctgct accgcctgta agctggaggt
    24061 ggtttccagc tggaagctcg acagttccag tacgtacagt tcgcactcat catccagcag
    24121 catcaacgcg ggcaggccaa tattgccacc cacaccaacg ttaacccccg ccgctttcgc
    24181 catttcaccc actagcgtgg tgaccgtgct tttgccgtta gaaccggtaa tcgccacaat
    24241 cggtgcttgt gcttcgcgac agaacagctc gatatcgcca acgatttcga ttccggcatc
    24301 agcggcagcg cttaaggatg gatgcgccag tgcaataccg ggactggcga caatcagatc
    24361 tgccgccatc agccattcat cattcagact gcccgtgtgg cgttctacgg cttcgggtaa
    24421 tttatccagg ccaggcggtg tcatacgcgt atccataacg cgcggcgtca caccgcgagc
    24481 gaggaaaaag tccacgcagg aaagcccggt gaggcccagg ccgataatga caacattttt
    24541 accctggtaa tcagccatga ttaacgtacc ttcagcgttg ccagaccaat caggaccagc
    24601 atcagcgaaa taatccagaa acgcacaatg acgcgcggtt ccggccagcc tttcagttca
    24661 tagtggtgat gaatcggtgc catgcggaaa atacgttgtc cgcgcagttt aaaggagccg
    24721 acctgcagga tgacggaaag cgtttctacc acgaacacgc cccccataat caccagcagg
    24781 aattcctgac gcagcagtac ggcgataatg cctaacgcac cacctaacgc cagcgaacct
    24841 acatcgccca taaagacctg cgccggatag gtgttaaacc acaggaagcc cagccctgcc
    24901 ccgactatcg cggtacagac aataaccagt tccccggcgt gtcgcagata tggtatgtgt
    24961 aaatagctgg caaagttcat gttgccggtc gcccacgcca ccagcgcaaa accaccggcg
    25021 acaaatacgg tcggcataat tgccagaccg tcaagaccat cggtcaggtt taccgcgttg
    25081 ccagtaccca caatgacgaa gtaagccagc agaatgtaga acagccccag ttgcggcatc
    25141 acatctttaa agaatgggac caccagctgc gttgcgggcg tgtctttgcc aacaaggtac
    25201 agcgcgaagg cgacacccag cgcaatgacc gacatccaga aatacttcca acgagcgatc
    25261 aaccctttgg tgtctttacg cactactttg cgataatcat caacaaagcc aataacaccg
    25321 taacctacca gcaccaccaa cacgcaccag acgtacggat tggacgggta agcccacagc
    25381 agtacggaga tcacaatcgc cgtcaggatc ataatcccgc ccatggtcgg cgtaccgcgc
    25441 ttgctgaagt gtgattcagg accgtcgtta cgcaccacct gaccaaagga aagtttttgc
    25501 aaatgagcaa tcatacgcgg gcccatccac aatgagatga acagcgcggt cagcaggctg
    25561 acgatggcgc gaaacgtcag ataggaaaag acgttaaagc cggaataata tttgaccaaa
    25621 tgttcggcca gccaaactaa catgtcccat tctcctgtaa agcgcgtact acctcttcca
    25681 tggcggcact acgtgaaccc ttaactaaaa tcgtaattac ctgttgctca gcaatcagtg
    25741 atttaagacg cgtaattaac gcagttttct cagcaaaatg ttcgccaacg ccgctggcgg
    25801 tgctgatagc atggctttgt ttacccacgc ttaacacgcg gtcaatacca gcagcttttg
    25861 ccgcctcgcc cacctgtaca tggcaggctt cgctttcagc gcccagttcc gccatatcgc
    25921 ccaccaccag cacgcggtag cccggcattt cagccagtac ctgaacagcg gcggtcattg
    25981 aaccgacatt ggcgttgtag gagtcgtcga gcagcaactg gttttctgcc agttgaatgg
    26041 ggaacaaacg gcctggaata gctttcagat ttgccagccc cgctttgata gcatcaagcg
    26101 ttgcgcccac ggacatggag agcgcagcgg ctgccagcgc attcgcaata ttgtgacgcc
    26161 ccggcaacgg cagcagaaca tccacgctac cggttggggt ttgcagggta aattccgtac
    26221 cgtgcgaagt cacatggata ttggtggcgg tgaaatcgct gttggcggca ttaggtgaga
    26281 aacgccacac tttgcgtgag ccaattacgc tctgccagtt cagccagtcg ttgttgtcgg
    26341 cgttcataat ggcgataccg ttttccggca ggccgctaaa gatttcacct ttcgctttcg
    26401 cgacacccgc aagcgagcca aaaccttcca gatgcgccgc tgccaggttg ttgaccagcg
    26461 cagcttccgg gcgagtcaga ctcacagtcc agacaatttc gccctgatgg ttcgcgccaa
    26521 gttcaataac tgcgtaatcg tattccggcg ttaagcgcaa cagcgtcatc ggtacaccga
    26581 tgtcgttgtt gagattgcct gccgtataaa gcgtgttgcc gcactggctt aaaatcgccg
    26641 ccgtcatctc tttaacggag gttttgccgg aggaacctgt cagagcaacc acgcgcgccg
    26701 gaacttgctg gcgaacccat gcagccagtt caccaaacgc cagacgcgta tccttgacga
    26761 ttaactgcgg caggtcgata tccagcggac ggctaaccag tagtgcgcct gcgccgccag
    26821 ctttcgcctg gtcggcaaaa tcatgagcat cgaaacgttc gcctttcagg gcaacaaaca
    26881 ggcagcccgg cgtcagtttt cgggtatcag tggttacagc atcaagggtg atatctgcac
    26941 cttgcagttc accgttgaga atgtcggtaa gttggctaag ggttacgcta atcatgcaat
    27001 cacccccagc agacgcgcca ccgtgacgcg atcggagtag tccagacgct gattgccaac
    27061 aatctggtaa tcttcatggc ctttgcccgc gaccagcacc acatcgttct ctttagcctg
    27121 cataacggcg caagtcaccg cttcagcacg gtcttccatc actttggcat gtccggcatc
    27181 taacattccc gccagaatat cgttgatgat ggcacgcggc tcttcggtac gcggattatc
    27241 gtccgtcacc accgccacat cagcaaactc ttcggcaatt gcgcccatca gtggacgctt
    27301 acctttatcg cgatcgccac cacagccaaa gacacaccac agcttgcccg cacagtgcag
    27361 acgcgccgcc tgtaaggctt tttccagtgc atccggcgta tgcgcgtaat ccaccaccac
    27421 cgtcggtttg cctggcgcag tgaacacttc catacgtccg caaaccggtt gcagacgagc
    27481 agcggttttc agcagatcag ccagtggata gcccagcgcc agcagcgtcg ccagcgcgag
    27541 cagcaggttg ctgacgttaa aagcgcccat cagatggctt tcaatttcgc catcgcccca
    27601 acttgagcta aagcgaatag tcgcaccgct gtcgtgatag ttcacttcgg tcgctttcaa
    27661 ccagcgtccg tgacagttcg gattgatatg atcttccatt gataccgcaa ctgcatccgg
    27721 cagttttgcc agccagcggc ggcctacttc atcgtcggca ttaataatcg cctgaccgca
    27781 atgatgttca gaataaagca gccatttcgc agcttcgtag tgttccatat caccatgata
    27841 atcaaggtga tcgcggctta agttagtaaa gaccgacgcc gcaaatttca atgccgccac
    27901 acggtgctgt accagcccgt gggaggaaac ttccattgcg caaaaagtcg cgtcctgatc
    27961 caccagcccc gccagctcat gctgaacatc aactgccgaa ccggtggtat tttctgtcgg
    28021 gatcactttc cccagcagac cgttaccaac ggtgcccatt accgcgctgg tttcgccaag
    28081 cagttggctc cactgcgcca gcagctgggt cgtcgtggtt ttgccgttgg tgcccgttac
    28141 gcccacgaga cgtaaattgt cagagggttc atggtaaaag cggcccgcca gtgcagataa
    28201 acgctcgttg agctggctga gatagatgac cggtacgccg tgcatttcac ggatttcacc
    28261 gtcggtcgcc tcacctttcg cctctgcaat aatggcagcc acaccttgcg ctatcgcctg
    28321 cgggatatat cgacgcccgt ccgcctgatg acctactaca gctacaaaga gatcgcccgc
    28381 cgcagccaca cggctgtcga gtgtcatctc tcgcagtgct cgcgaaggtg cgtctggcac
    28441 ccacggagca agaaggtcgc gcaaattacg atctgccacc tgtcccctcg ccttgattaa
    28501 tcacaaattc atttttatcg cccgttgtca gcgcatccgg ctcgatgttc atggttcgca
    28561 atacgccgcc catgatggca ccaaagaccg gcgcggaaac ggcgccgccg tagtatttac
    28621 ccgcctgcgg atcgttgata acaacaacca gcgcgaagcg cggctgactc gcaggcgcaa
    28681 cacctgcggt gtaagcaata tatttattga tgtagcgacc gtccggcccg acctttttcg
    28741 cggtaccggt tttaatggca atacgatagc ctttaatcgc cgccttcacg ccgccgccac
    28801 ccggtagcgc cacgctttcc atcatatgca ccacagtgcg gacaatagat tccgggaaga
    28861 cacgttcacc gggaaccggg gggtcaactt tggtaatcga cagtgggcga taaatgccgt
    28921 agctgccgat agttgcgtag actcgcgcta actgtaacgg agttaccatt agcccgtagc
    28981 cgaaagagaa ggtggccctc tctatgtcag accaccgttg tttttgagga tataagccac
    29041 tgcgttctcc gaccaacccc aaattggtcg cttttcccag tccaaaacgt gagtaagtat
    29101 ctactaacgc tgaggacggc atcgctaacg ccagcttgga aacaccgacg ttactcgact
    29161 tctgtaatac tccggtcagg gttaattcgc tgtagcgtgc cacgtctttg atttcgtggc
    29221 cgttaattcg ataaggaatg gtattgagta ccgagttttc ccgcaccacg ccacgttgca
    29281 acgcggtcat caccaccatc ggtttaaccg ttgaacccgg ttcaaacacg tcggtgatgg
    29341 tacggttacg catcgcctct ttcggcgtgc cgctcagatt gttagggttg tatgacgggc
    29401 tgttagccat cgccagcact tcaccggtgt tgacatccac cagcacggcg ctaccggact
    29461 cagccttgtt aaaggccacc gcgttgttca gttcgcgata aaccagcgcc tgcaggcgtt
    29521 catcaatact cagcgccagg ttgtgcgcag cctggctgtc agtagaagaa atatcttcaa
    29581 ttacgcgacc atagcggtct ttacgcacaa tgcgctcacc cggctgcccg gtaagccatt
    29641 tatcgaaact cttctcaacg ccctcaatac cttgactatc gacgttagta aagccgatga
    29701 ggtgagcagt cacttcgccg gatggataat aacggcgaga ttcttcacgc agatgaattc
    29761 ccggcagttt cagttttttg atgtagtccg ccatgtcagg gtttacctga cgcgccagat
    29821 aaataaagcg ccctttcggg ttggcgttaa tgcgggcaga aagctgatcc agcggaatat
    29881 tgagcgcgtt agccagcgcc ttccagcggt caccgacgct gataccgcca gcgtcatgca
    29941 cttctttcgg gtcagcccaa atcgctttta ccggcacgct caccgctaac gggcgaccag
    30001 aacggtcagt aatcatgccg cgggaggtgg aaacttgctg aacgcgaaga gaacgcatgt
    30061 cgccctcttt caccagcata tccggggaga taacttgtaa ccacgctacg cgtccgagca
    30121 gaaaagccag cgccaggaga atacagccgc ataacaacgc aaaacgccaa ctgataaagt
    30181 tggcatgttc ttcctgacgt tttggtttct gcgttttcgc cgctgctttc atgcgtcgcg
    30241 tttatcctta tttttgcact acgatatttt cttgtgacgg atcaacatgc tgcatttgca
    30301 gcttttccgt ggcgatcctt tccacccggc tatggtcgcc gagcgcattc tcttcaagga
    30361 tcaggttgcg ccattcaatg tctaaagcat ctcgctccag caccagttgt tcgcgctgag
    30421 cggtcagtaa acgggtatgg tgcgccgtgg ttaccacggt caccgccgtc aaaataatgc
    30481 aaatgaacag gcagagtggc agcttcccaa atcgcaaaag atcgtcaccg ataacaccag
    30541 gcaatgcatg gcgctcgtgg cttcccatcg atcctttaac tttgcttaga gcttctgtca
    30601 ctctgctgat catgcgttcg tcctctctgc aatacgcaga actgaactac gggcacgagg
    30661 attctcagcc acctcttctt cgcccggcat taatttgcct agtgctcgca gctgacggcc
    30721 acccagtttt ttgagctgct cttcagtcat cggtaaccct gccggaactt gcggaccgcg
    30781 gctgttttca cgcataaaac gtttcacaat acggtcttcc agcgagtgga agctgatgat
    30841 cgaaagccgc ccacccgggg ccagcacgtt gagcgagctt tttagcgcct gctctatctc
    30901 ctccagttca ctgtttaccc aaatgcgcac cgcctggaag gtacgggtcg cgggatgttt
    30961 aaacttatct ttcaccggcg ttgcagcagc cacgacttcc gccagttctt tggtgcgggt
    31021 catcggctgt tcgcggttac gctcgacaat ggctcgggca atgcgtttgg caaaacgctc
    31081 ttcaccatag gttttcaata cccaggcgat atcggcttct tctgcggttt gtagccattc
    31141 agcggctgac tgcccacggg ttgggtccat acgcatgtcc agcggaccat cgcgcataaa
    31201 ggaaaagccg cgttcagcat catcaagttg cggtgaagag acaccaagat cgaggagaat
    31261 gccgtcgatc ttgccgataa gatcgcgctc ggcaacgtat tcgcccagcg cggaaaaagg
    31321 tccgtggatg atggagaagc gcggatcatc aatagtcttc gcaacggcga tagcctgcgg
    31381 gtcgcgatcg atcgccagca aacgcccctc ttcgccaagc tgcgagagga tcagacgtga
    31441 gtgaccaccg cgaccaaaag tcccatcaat gtagatgcca tcaggacgga tattgaggcc
    31501 attaacggct tcatccagca gcaccgtagt atgtttatag ttttccatca ttttatagag
    31561 acaagtcctg cagtcgctcc gataagtctc cggtagccaa ctgctctgcg tcgatatctt
    31621 ccttgacctg ttgatgccag gttgtttcat cccacagctc aaacttgttg aactgtccaa
    31681 ccagcatcac ttcttttgtc agcccggcat gttgccgcag tactggcgcg attaacaatc
    31741 gacctgcgcc atccatctga cattcgctgg catgacctaa cagtaggcgc tgcacacggc
    31801 gctcaaccgg gttcatgctc gacagacgcg ataatttttg ctcgataatt tcccattcag
    31861 gcagggggta aagcagcagg cacgggtgat gaatgtcaat ggtgcaaacc atttgaccgg
    31921 cagcgttctc aagcagctgt tcccgataac gggtaggcac tgataagcgc cctttgctgt
    31981 cgagattgac taacgttgct ccccggaaca tgccagcctc accccttatt cccactttgc
    32041 cccacaattt cccactgaaa ggagtttacg gagcggagga aaagcttgtc aagccagtca
    32101 caaggcatac cgaggtaaaa aacccgatgt ttacggctaa tatcagcgtt gtttatttta
    32161 cgtccaacgt gcgaagcaaa ttaacgttat gaatagctgt aagaaaaaaa ccctcatcgt
    32221 catcaccgtt ttaaaatgac tcactatttt ccacagcaaa tataaagtgt cagtttgcga
    32281 cgcgagcggc attttaggac atatcttccc cggttaacag tccctgttgc gtctgtgtag
    32341 cgcttgatgc cagcgggttt gcgcaaaagc aaagaaaagt gcttgttaaa tcggcaattc
    32401 atctctgcat gtaacaaaat aatgcaaaaa cgcgctgtcg atatcactta attattgtga
    32461 atttcagctg gtgagttaat taactggcgg gtttaaaagc atttatcaat aatagtcagg
    32521 aatagtctta tttactttaa ggatattgat gtccagtccc gtactctacg cgccagagtg
    32581 aaattcacct ggcgcgtatt tttgttcgca gcttagctac ggctgagcac accgcggcga
    32641 tagagattac gtttaatgcg cgttaaacca ggttttggct tacgcggttc gtccaggctt
    32701 gccaggacaa tctccagcac acgctctgcg acatcgcggt gacgttgagc cactgccagc
    32761 accgggcact gtaagaagtc gagcagttcg ttatcgccaa aggtggcaat tgccaggtca
    32821 gaaggcagtt taccatcgcg acgtagcgtg acatccatca ccccttgcaa caacgcaaac
    32881 gacgttgtga acagcgcctg cggcatcgga tgcgtttcca gccatttttc gaataactgg
    32941 gcagccgcct cccgctcata gctgttggca tacaggaaat gcacttcgcg cggatcatct
    33001 ttccaggcag tacggaaacc ttgttcacgc aggaagctga cagaaagctc cggcagcgcg
    33061 ccaagataaa gcaccgtctc ggcgggaaac ttacgtaact cttccgccag catttcggca
    33121 tcatcctgat cggcaccaac cacgctggta aagtgttcac gatcgagggc gcggtccagc
    33181 gcgacaatcg ggaacgggtc gttagcccag cgttgataaa aaggatgctc aggaggcaac
    33241 gacgtcgaaa caataatggc atcgacctga cgctgtaaaa ggtgctcaat gcagcgcatt
    33301 tcgttgtctg gctgatcttc tgagcaggca atcagcagtt gataaccccg ttgccgcgcc
    33361 tggcgttcaa gatagttagc gatgcgggta tagctggtgt tctccagatc ggggatcaca
    33421 agaccaatag aacgtgtgcg tccagcacga agcccagctg ccacggcgtt cgggtggtaa
    33481 ttgtgctcac gcaccacagc catgactttt tcaacggttt tgtcactcac acggtattgc
    33541 ttcgctttgc cgttaataac atagcttgca gtggtccgcg acactcccgc cagccgagcg
    33601 atttcatcca gtttcacaat tgccccttgc gtaaaatgta aaaaccatag ccattgtact
    33661 ggtatgggtt aaattcatta acatctaagc gcagaaaatt aactgcggca actgctttta
    33721 ttccaggttc ccactgattt cgcaaaaaaa gcccaacgtg acatgttggg ctgtaaattg
    33781 cgcattgaga tcattctcaa cgcattattt tatcgccgcg cgaaagtccg accacaccag
    33841 agcgagcaac ttccacaatt ttcgccacat cgcgaatcga tgctaaaaat gcatcaagct
    33901 tatcgctggt gcctgctaat tgaacggtat aaagcgaggg tgtgacatca ataatttgcc
    33961 cacggaatat ttccgtatta cgtttaactt cgtcacgccc gtaaccgctg gcctgaattt
    34021 tcaccagcat gatttccctc tcaacatgcg cgccctgccc caactcactc acgcgcaaga
    34081 catcgaccag cttgtataat tgcttttcga tctgctcaag cactttttca tcgcccacgg
    34141 tctggatggt catacgcgat aatgtcggat cgtcggttgg cgcaacggtc aggctttcaa
    34201 tgttgtagcc acgctgggaa aaaaggccaa tcacgcggga taacgcgcct gattcatttt
    34261 cgagtaagac tgataatatc cggcgcataa tcaggttctc tccgttttgc ttaaccacat
    34321 ttcatccatt ccgcccccgc gaatctgcat cgggtagacg tgctcactgc catcgacggt
    34381 gacatcaaca aataccaggc gattattacg cacctgttca agcgcctcgc taagtttgct
    34441 ttccagctct tgcggatgag agatctggat cccgacgtga ccataggctt ccgccagacg
    34501 gacgaagtct ggtaaagact gcatataaga ttgtgaatga cgaccggaat agatcatgtc
    34561 ctgccactgc ttcaccatcc ccaaatagcg gttattaaga ttaaccacca gcaccggcag
    34621 ttcatattgc aacgctgtag acagttcctg aatgttcatc tgaatactgc cgtcgccagt
    34681 gacgcaaacc acggtttctt ctggcagcgc cattttgacg cccagtgccg caggtaaacc
    34741 aaagcccata gtgccgaggc caccggagtt gatccaacga cggggtttgt cgaatggata
    34801 ataaagcgca gcaaacatct ggtgctgccc gacatcggac gtaacgtaag cgcccccctt
    34861 cgtcaaccgc caaagagtct cgatcaccgc ctgcggttta atcttttcac tgtgagtgtc
    34921 atatttcagg cactgacgag cgcgccactg ttcaatctgc tgccaccagt cgcggatctc
    34981 atccagcggt tgatgggcgg attcttgcga caagagttca agcatttgtt cgaggacctg
    35041 gcgagcatcc cccacaatcg gaatatccgc agtcacggtt ttagaaatgg aagtaggatc
    35101 aatatcgata tgcagaacag tggcatttgg gcagtacttt gccagattgt tagtggtgcg
    35161 atcgtcaaag cggacaccga cagcaaaaat cacatccgcg ttatgcatcg tcatattggc
    35221 ttcgtaggta ccgtgcattc ccagcatgcc cagtgcctga cgatgcgttg ccggaaacgc
    35281 tcccagcccc atcaatgagg aaacaacggg cagatttaac gcctccaccg tttctttcag
    35341 ctgttgatgg cagcccgccg tgattgcccc accgcctacg tagacaaccg gtttttttgc
    35401 cgccaccagc gtttgcagag cgcgcttaat ttgcccttta tgtccggtag tagtgggatt
    35461 gtaagaacgc atactgaccg actccggcca gacatagggt aatttgttcg ccggattaag
    35521 aatatctttc ggtaaatcaa cgaccactgg tcctgggcga ccacttgccg ccagccagaa
    35581 agcctttttc agcacctgcg gaatgtcttc cgtttgctta accagaaaac tgtgtttaac
    35641 caccggtcgc gaaatcccca ccatgtcgca ctcctgaaag gcatcgtaac ctatcaacga
    35701 ggtcgctacc tgcccggaaa ggacaactaa tggaatggaa tccatataag cggtggcgat
    35761 gccggtaatc gcattggtcg cccctggacc cgacgttacc agcacgacgc cgacttcccc
    35821 ggtcgcgcgc gccagaccat cggccatatg cactgccgcc tgctcatgac gaactaaaac
    35881 atgaccgata ccacccacgg tatgcaatgc atcgtaaata tcaaggactg cgcctccggg
    35941 ataaccgaat acttgtttaa cgccctgatc gataagcgat cggacgacca tctcggctcc
    36001 agacaacatc tccatggcct gcctcactgt ttgacggaaa aaatgtgtaa aacacattaa
    36061 ccgtccgatc ttgcttaagc aattggcagc cagagacata aacgccgggt aataagaata
    36121 aacaggagga aaggtgaaaa gaaacgataa aatcctccat tacgaaggat aaaaatgata
    36181 ataaatcggc taaatcccac cacttacagc aatttctcta tttttttaat taatcctaaa
    36241 atagagagcc cacacattca gaataaaatt ctgcactcaa taaaaaatgc agaataaacc
    36301 agacattcat gtctgaccta ttctgcattc agttagcgtt tgcaaattga gactaattgc
    36361 tcttccatcc actgatggcc tttatcacgc ccggcagctt catgccagga gagataacag
    36421 gttctgctat ttagttttaa cggcagcggt aatacctgta attctaagga ttcagcgaac
    36481 tcttcagcca gccaacgcgg cgcaatagcg accaaatgcg tttgcgacac cacgctaagt
    36541 acgctcatca ttgccatgcc ctgatacgcg atactggctt gcttatctac cgtgtcatac
    36601 caaggttgac taaatgacgc gaaacgatcg agcgaaaccg ccgcatgttg ttcgttataa
    36661 acatcatgtt tcagtaacgg gcccttaatt gttgggtgat ttttgctggc taccagcacc
    36721 atttcatctt taaataatgg cacgctggta aattcaggac gatggaagtc ttcataacta
    36781 atcacaaact ccgtttcctg ataacgcagc tgatgttcag tgttctgatt taatgaagac
    36841 ttgaacataa catgtatatt tggcgcaatc tgctcaatgt gattataaat ctgcgaggtc
    36901 agaatactgt ctaacgggct gcaaacacaa agatgaaata cacgttcact gctcgcgggt
    36961 tcaaaacctg aaccaggcaa ttcattttgt actagttgca atgcctgacg aactgaacca
    37021 aaaagttgaa atgcgcgagc agtcggttga ataccacggc cataacgaac aaaaagctcg
    37081 tcattaaaca tcaccttcag gcgtgcaaca gcgttactga ccgcaggttg cgacattccc
    37141 agaacatgag cagcacgggt aatgttttgc tcctgcatca cggcatcgaa aacggttaat
    37201 aagttgagat cgaccatgcg tagctgtggc ttgctgaact ccgccgtctc tggatgatct
    37261 gtttgtacct ctggcatact taactccact gtcacactta actccctttc ccttattgga
    37321 atgcagagaa ataatcctcg agaatatgat ttatcatgca tataaaataa gaaaaaggaa
    37381 aatgagtaaa attaggaaaa cataaagata cgggttctta taaaaacaga atccattgcc
    37441 gtggaaagcg tcatttacca cagcataata atccataatg ttcttgttgc gtaaacaatc
    37501 taatcataac taccgcgaat actcaatcat ccacaaaatg gattaaatgt gctttttaat
    37561 aatttattta ta
//
LOCUS       DAZFDC010000030        36495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFDC010000030 DAZFDC010000000
VERSION     DAZFDC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.030"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(206..1798)
                     /gene="lsrK"
                     /locus_tag="ACHJ48_001672"
     CDS             complement(206..1798)
                     /gene="lsrK"
                     /locus_tag="ACHJ48_001672"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO4707250.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSTIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1877..2830)
                     /gene="lsrR"
                     /locus_tag="ACHJ48_001673"
     CDS             complement(1877..2830)
                     /gene="lsrR"
                     /locus_tag="ACHJ48_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO4707251.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3079..4614
                     /gene="lsrA"
                     /locus_tag="ACHJ48_001674"
     CDS             3079..4614
                     /gene="lsrA"
                     /locus_tag="ACHJ48_001674"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO4707252.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITSADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLELYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     AAC"
     gene            4608..5636
                     /gene="lsrC"
                     /locus_tag="ACHJ48_001675"
     CDS             4608..5636
                     /gene="lsrC"
                     /locus_tag="ACHJ48_001675"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO4707253.1"
                     /translation="MLKFIQNNREITALLAVLLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALELNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5636..6628
                     /gene="lsrD"
                     /locus_tag="ACHJ48_001676"
     CDS             5636..6628
                     /gene="lsrD"
                     /locus_tag="ACHJ48_001676"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO4707254.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGAIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6640..7662
                     /gene="lsrB"
                     /locus_tag="ACHJ48_001677"
     CDS             6640..7662
                     /gene="lsrB"
                     /locus_tag="ACHJ48_001677"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO4707255.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7689..8564
                     /gene="lsrF"
                     /locus_tag="ACHJ48_001678"
     CDS             7689..8564
                     /gene="lsrF"
                     /locus_tag="ACHJ48_001678"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO4707256.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGTQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8588..8878
                     /gene="lsrG"
                     /locus_tag="ACHJ48_001679"
     CDS             8588..8878
                     /gene="lsrG"
                     /locus_tag="ACHJ48_001679"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO4707257.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8935..9693
                     /gene="tam"
                     /locus_tag="ACHJ48_001680"
     CDS             8935..9693
                     /gene="tam"
                     /locus_tag="ACHJ48_001680"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO4707258.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLKEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9697..10611)
                     /gene="yneE"
                     /locus_tag="ACHJ48_001681"
     CDS             complement(9697..10611)
                     /gene="yneE"
                     /locus_tag="ACHJ48_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO4707259.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQVEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKVLPDRHYQLT"
     gene            complement(10808..12259)
                     /gene="uxaB"
                     /locus_tag="ACHJ48_001682"
     CDS             complement(10808..12259)
                     /gene="uxaB"
                     /locus_tag="ACHJ48_001682"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO4707260.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12486..13904)
                     /locus_tag="ACHJ48_001683"
     CDS             complement(12486..13904)
                     /locus_tag="ACHJ48_001683"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO4707261.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14043..14402)
                     /gene="yneG"
                     /locus_tag="ACHJ48_001684"
     CDS             complement(14043..14402)
                     /gene="yneG"
                     /locus_tag="ACHJ48_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO4707262.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPHGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14402..15328)
                     /gene="glsB"
                     /locus_tag="ACHJ48_001685"
     CDS             complement(14402..15328)
                     /gene="glsB"
                     /locus_tag="ACHJ48_001685"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO4707263.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15392..16780)
                     /gene="sad"
                     /locus_tag="ACHJ48_001686"
     CDS             complement(15392..16780)
                     /gene="sad"
                     /locus_tag="ACHJ48_001686"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO4707264.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVTQMIKDSRIAAITVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMDDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16881..17762
                     /gene="yneJ"
                     /locus_tag="ACHJ48_001687"
     CDS             16881..17762
                     /gene="yneJ"
                     /locus_tag="ACHJ48_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4707265.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17840..18955
                     /gene="yneK"
                     /locus_tag="ACHJ48_001688"
                     /pseudo
     CDS             17840..18955
                     /gene="yneK"
                     /locus_tag="ACHJ48_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            19106..20296
                     /gene="ydeA"
                     /locus_tag="ACHJ48_001689"
     CDS             19106..20296
                     /gene="ydeA"
                     /locus_tag="ACHJ48_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO4707266.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGTVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20321..20986)
                     /gene="marC"
                     /locus_tag="ACHJ48_001690"
     CDS             complement(20321..20986)
                     /gene="marC"
                     /locus_tag="ACHJ48_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO4707267.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TNH"
     gene            21198..21632
                     /gene="marR"
                     /locus_tag="ACHJ48_001691"
     CDS             21198..21632
                     /gene="marR"
                     /locus_tag="ACHJ48_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO4707268.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWIERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21652..22035
                     /gene="marA"
                     /locus_tag="ACHJ48_001692"
     CDS             21652..22035
                     /gene="marA"
                     /locus_tag="ACHJ48_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO4707269.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22067..22285
                     /gene="marB"
                     /locus_tag="ACHJ48_001693"
     CDS             22067..22285
                     /gene="marB"
                     /locus_tag="ACHJ48_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO4707270.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(22316..23215)
                     /gene="eamA"
                     /locus_tag="ACHJ48_001694"
     CDS             complement(22316..23215)
                     /gene="eamA"
                     /locus_tag="ACHJ48_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO4707271.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAISGVLVLIEDSFNGQHVAMLGFMLTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            23410..24597
                     /gene="ydeE"
                     /locus_tag="ACHJ48_001695"
     CDS             23410..24597
                     /gene="ydeE"
                     /locus_tag="ACHJ48_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO4707272.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLIGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAVNPLVSGVVLTTLPSSSLFIILALVIIAAWALMLKGINARPQRQP
                     ALC"
     gene            complement(24638..25033)
                     /locus_tag="ACHJ48_001696"
     CDS             complement(24638..25033)
                     /locus_tag="ACHJ48_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO4707273.1"
                     /translation="MRYVTEFTTAKPEVAEAHFLARLSVETDCADVHDSLAYIGDDFV
                     LLHVVGREEDFARRHIPGALHLPHKQMTAERMAEWPDDTLFVVYCAGPHCNGADRAAL
                     KLARLGKPVKIMLGGITGWEDEGYAFASE"
     gene            25128..26099
                     /gene="ftrA"
                     /locus_tag="ACHJ48_001697"
     CDS             25128..26099
                     /gene="ftrA"
                     /locus_tag="ACHJ48_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtrA"
                     /protein_id="HFO4707274.1"
                     /translation="MTTLQRPLVIALAYDGLCTFEFGVAVEIFGLPRPELGDNWYRFA
                     VAAVEPGALQATGGIHVVADGGLELLPNADMIIIPGWRGIDAKVPEMLCQSLREAHQR
                     GTRLVSICSGVFVLAAAGLLHQRKATTHWRYTEQLQHRYPSISVSDNVLYQDEGQVMT
                     SAGSAAGIDLCLHIVRKDFGHEIANNVARRLVIQPHRQGDQPQNLTRPMASPRESQTL
                     GALFDFLQQNLAQTHTVSSLAERVNMSPRTFLRRFEALTGMTPARWLLNERLIRVQDY
                     LQNSVFNIEKIAELTGFGNSALLRHHFHQRFLLSPSQYRKNSRSPSQ"
     gene            26207..26302
                     /gene="mgtS"
                     /locus_tag="ACHJ48_001698"
     CDS             26207..26302
                     /gene="mgtS"
                     /locus_tag="ACHJ48_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO4707275.1"
                     /translation="MLGNMNVFMAILGIILFSGFLAAYFSHKWDD"
     gene            26302..26406
                     /gene="mgtT"
                     /locus_tag="ACHJ48_001699"
     CDS             26302..26406
                     /gene="mgtT"
                     /locus_tag="ACHJ48_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO4707276.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(26521..27411)
                     /gene="dgcZ"
                     /locus_tag="ACHJ48_001700"
     CDS             complement(26521..27411)
                     /gene="dgcZ"
                     /locus_tag="ACHJ48_001700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO4707277.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEETLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(27666..28058)
                     /gene="ydeI"
                     /locus_tag="ACHJ48_001701"
     CDS             complement(27666..28058)
                     /gene="ydeI"
                     /locus_tag="ACHJ48_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO4707278.1"
                     /translation="MKFQAIVLASFLVIPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPATVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            28334..28852
                     /gene="ydeJ"
                     /locus_tag="ACHJ48_001702"
     CDS             28334..28852
                     /gene="ydeJ"
                     /locus_tag="ACHJ48_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO4707279.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(28897..30942)
                     /gene="dcp"
                     /locus_tag="ACHJ48_001703"
     CDS             complement(28897..30942)
                     /gene="dcp"
                     /locus_tag="ACHJ48_001703"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO4707280.1"
                     /translation="MTTINPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            31079..31825
                     /gene="ydfG"
                     /locus_tag="ACHJ48_001704"
     CDS             31079..31825
                     /gene="ydfG"
                     /locus_tag="ACHJ48_001704"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO4707281.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            31914..32600
                     /gene="rspR"
                     /locus_tag="ACHJ48_001705"
                     /gene_synonym="ydfH"
     CDS             31914..32600
                     /gene="rspR"
                     /locus_tag="ACHJ48_001705"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO4707282.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            32777..32980
                     /gene="ydfZ"
                     /locus_tag="ACHJ48_001706"
     CDS             32777..32980
                     /gene="ydfZ"
                     /locus_tag="ACHJ48_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO4707283.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(33016..34476)
                     /gene="ydfI"
                     /locus_tag="ACHJ48_001707"
     CDS             complement(33016..34476)
                     /gene="ydfI"
                     /locus_tag="ACHJ48_001707"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO4707284.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLHQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(34565..35848)
                     /gene="ydfJ"
                     /locus_tag="ACHJ48_001708"
                     /pseudo
     CDS             complement(34565..35848)
                     /gene="ydfJ"
                     /locus_tag="ACHJ48_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            35980..36222
                     /locus_tag="ACHJ48_001709"
     CDS             35980..36222
                     /locus_tag="ACHJ48_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4707285.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(36219..36353)
                     /locus_tag="ACHJ48_001710"
     CDS             complement(36219..36353)
                     /locus_tag="ACHJ48_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFO4707286.1"
                     /translation="MKLILGTACKTRHLVCKNVLRLAGELSIVRVLNDFQPLLILLVN
                     "
     gene            complement(36384..>36495)
                     /locus_tag="ACHJ48_001711"
     CDS             complement(36384..>36495)
                     /locus_tag="ACHJ48_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4707287.1"
                     /translation="TRAAHPLSRESIAVSMIVGRDNCAFDSDRGNFVLKN"
BASE COUNT         9398 a         9172 c         8668 g         9257 t
ORIGIN      
        1 tgtctttagg aatattcgtt ataaaataag ggttatgctt aatgctttaa gaaaaatagc
       61 aatttcccct tgaatatagt actggtgatg aaacgatgaa tctgcaagct ggcatttaac
      121 aagccagctc taaaagaagg gaaataagaa taactatact caaaaactaa cagccacggt
      181 catcatgatg tggctgtcaa tgaaactata acccaggcgc tttccataac gacgtcgtca
      241 gtccatgatc aaccagcccc agctgatcct gataaactgc ctgccactta tcgcgtgaat
      301 cctgataaag ttcatgcttt tccgggtctg gtgtgtgcgt ccgttcccag cgaaccaggc
      361 gttctccggt ttctgccatt gatgaaaaaa ttccggcacc gacgccagct gcaatggcac
      421 atcctaatgc agtggcttct ttgaccaccg gaatattgac gggtaatccc gagacatcag
      481 cgagaatttg actccataat ttcccttttg aacctccgcc tgcaaagact aacgatgaag
      541 gatgaatagt cgaaaaatca gcaatttgct gcaagttaca cgctgataca atcgccgcat
      601 tttcttccag cgcacggaac aatgtcgctt tgttacattt atccggatca atggacaaat
      661 taataaagga aggcgcagcg tgataccagg ttttaaagcg cattctgtcg gagaagatcg
      721 gcattacgcc ccacgaccca ggcggcaccc gactggccat ctcttccagc agcgtatagg
      781 tgtcgatgcc taaacgttcg gcaatcagtt tttcttcggc acagaaagca tcgcggaacc
      841 agcgcatggt gagtccggta aaaaagctta tagattcagc ttgtaccatg ccaggaataa
      901 catgaggatt aacgcgcacg ttcatttctg ggtctgtcac cggcgcggct aaatttacaa
      961 cttgctgcca gaatgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
     1021 ccaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc accttcagac
     1081 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
     1141 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc cagtcacgga
     1201 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgca agttcgccgc
     1261 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
     1321 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
     1381 cggtcgcgcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
     1441 cttcgcgcgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
     1501 tatataaaac aatgccttca cgcatcgaac atgccgaaac ggcagcgata tactccgggg
     1561 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1621 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1681 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1741 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1801 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1861 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1921 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1981 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagct
     2041 cgttatgtat tttaatgtcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     2101 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     2161 agcgaatgat cgtcgcgtcg tcttgttgac taacggcccc aatgccgaca atcgccacat
     2221 ccgctgcttg cgcggctaac agaacatctt tgacacaatt ttcatttttt agcgtacggg
     2281 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcat
     2341 taagctgtcc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     2401 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     2461 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     2521 tccccagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     2581 gcgaaaactg acgacgtaat tgtgtttcat attccagaca gccttcaaag cgagaattaa
     2641 tctgcacgcg aatgatgccg gactgatgcc ctttctccag taatcgcgac actttcaaac
     2701 gggtcaggcc gagacgatcg ctgatctcac tctgggtcag cccgtcgtgg tagtaaaacc
     2761 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt accgaatcgt
     2821 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2881 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2941 gcaaccgtga aaatcaaaat agtataaatt gtgatctatt cgtcggaaat atgtgcaatg
     3001 tccacctaag gttatgaaca aattaaaagc agaaatacat ttgttcaaaa ctcacctgca
     3061 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     3121 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     3181 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     3241 aagattattg ccggtattac ctctgctgat agcggtacgc tggagattgg gggcaacaac
     3301 tacgccagat taacgccagt tcatgctcat cagctgggta tttatctcgt tccccaggaa
     3361 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     3421 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     3481 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     3541 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3601 gaaaccgaac gcttgtttac tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3661 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcggattag cgtgatgcgc
     3721 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3781 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3841 ttacctggca accgcccaca acatgccgca ggaacgtcgg tgctgacact ggaaaatctg
     3901 actggcgaag gtttcaggaa tgtcagcctg acgctaaatg ccggagaaat tctgggtctg
     3961 gctgggctgg tgggtgccgg acgcacagaa ctggccgaga cgctctatgg tctacgtact
     4021 ttgcgtggcg gacgcattat gctgaatgat aaagagatca atagattatc cactggagaa
     4081 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     4141 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     4201 aaaaccgcga aagataatgc caccctggaa ctttatcgtc gggcgctgaa tattaaatat
     4261 aaccaaccgg aacaagctgc acggacatta tccggtggta accagcaaaa aatcctgatt
     4321 gccaaatgcc tggaagcctc gccgcaagta ttgattgtcg atgagccgac gcgcggcgtg
     4381 gatgtctcgg cgcgtaatga tatctaccaa ctgttgcgca gcatcgccgc acagaatgtg
     4441 gctgtgctgc ttatctcctc tgatctggaa gagatcgaac tgatggcaga tcgtgtgtat
     4501 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     4561 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcggcatg ctgaagttta
     4621 ttcagaacaa ccgtgaaatc acggcactgc tggcggtgct gctgctgttt gtattacccg
     4681 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4741 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4801 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4861 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4921 tcaacggtgt cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4981 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     5041 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg cgtttcagca attggttggt
     5101 taacgataat tctggtggca tttatggcct ggctgctggc gaagacagcg tttggacgca
     5161 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     5221 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     5281 ttgcttcgca gattggtttt atccccaacc agaccggtac cgggctggag atgaaagcaa
     5341 ttgcagcctg cgtgctgggc ggcattagtt tgctcggcgg ttccggtgcg atcatcggtg
     5401 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     5461 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     5521 gacgcctgcg ttgtgcgctg gaacttaatc tgcggcggca aaaatatgcc cgctttatga
     5581 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggct gcataatgcg
     5641 tattcgctac ggttgggaac tggctcttgc cgcactgctt gttattgaga ttgtctcatt
     5701 tggtgcaatt aacccgcgga tgttagatct caatatgttg ctgttcagca ccagtgactt
     5761 tatctgcatt ggcattgtcg ccctaccgct gacgatggtg attgtcagtg gcgggatcga
     5821 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5881 tggtgtgccg atgccgctgg cgattctcct gaccttactg ctcggcgcat tgtgcgggct
     5941 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     6001 gctgtatctg tttgccggaa gcgccctgct gctttccggg atggccgggg cgacggggta
     6061 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgttggg
     6121 gctccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     6181 taaaacccat gccggacgta acgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     6241 tagcgcgatt ccagttaacc gtaccttatg tgcgctctat gccatgacgg ggctggcgtc
     6301 tgcggtcgcc gctgtgctgc tggtgtcgta ttttggttca gcacgttccg atctcggtgc
     6361 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggtgccaata tttatggtgg
     6421 ttccggtgcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcaacaagg
     6481 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctacttat
     6541 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6601 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6661 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6721 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6781 ggcgcacaac aagcgggtaa agagctgggc gttgatgtga cctacgacgg gccgacagaa
     6841 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6901 attatcgttt ctgcggtttc gcctgatggc ttgtgtccgg cactgaaacg cgccatgcaa
     6961 cgtggtgtga gagtgctgac ctgggactct gatactaaac cggagtgccg ctcttactac
     7021 attaatcagg gaactcccgc ccagttggga ggtatgttgg tggatatggc ggcgcgtcag
     7081 gtgaataaag acaaagccaa agtcgcgttt ttctactcaa gccccaccgt tacggaccaa
     7141 aaccagtggg tgaaagaagc gaaagcgaaa atcgccaaag agcatcctgg ctgggaaatt
     7201 gtcactacgc agtttggcta taacgatgcc actaaatcat tacaaaccgc agaaggaata
     7261 ttaaaagcgt atagcgatct cgacgccatt atcgcccccg atgccaatgc cctgcccgct
     7321 gccgcacaag ccgcagaaaa cctgaaaaat gacaaagttg cgattgtcgg attcagtacg
     7381 ccaaacgtga tgcgtccata tgtggaacgc ggcacggtga aagaatttgg cctgtgggat
     7441 gtggttcagc aaggcaaaat ttcagtttat gtcgcggatg cattattgaa aaaaggatca
     7501 atgaaaacgg gcgacaagct ggatattcag ggcgtaggtc aggtggaagt ctcgccaaac
     7561 agcgtccagg gctatgacta cgaagcggat ggtaatggca tcgtactgtt accggagcgc
     7621 gtgatattca acaaagagaa tatcggcaaa tacgatttct gatgtgcatt acttaaccgg
     7681 agtaagttat ggcagattta gacgatatta aagatggtaa agattttcgt accgatcaac
     7741 cgcaaaaaaa tatccctttt accctgaaag gttgcggtgc gctggattgg ggaatgcagt
     7801 cacgcttatc gcggatattt aatccgaaaa cgggtaaaac cgtgatgctg gcttttgacc
     7861 atggttattt tcagggacca actaccggac ttgaacgcat tgatataaat atcgccccgc
     7921 tgtttgaaca tgccgatgta ttaatgtgta cgcgcgggat tttgcgcagc gtagttcccc
     7981 cggcgaccaa taagccggtg gtactgcggg cgtcaggtgc gaactctatc ctggcggaat
     8041 taagcaatga agccgtggcg ttatcgatgg atgacgccgt gcgcctgaac agttgcgcgg
     8101 tggcggcgca ggtttatatc ggcagcgaat atgaacatca gtcgatcaaa aatattattc
     8161 agctggtcga tgccggaatg aaagtgggaa tgccgaccat ggccgtgact ggcgtgggca
     8221 aagacatggt gcgcgatcag cgttatttct cgctcgcgac tcgaatcgcc gctgaaatgg
     8281 ggacgcaaat tatcaaaacc tattatgtcg aaaaaggttt tgaacggatt gttgccggat
     8341 gtccggtacc cattgttatt gctggcggta aaaaattacc ggagcgcgag gcgctggaaa
     8401 tgtgctggca ggctatcgat cagggcgctt ctggtgtgga tatggggcgt aatattttcc
     8461 agtctgacca tccggtggcg atgatgaaag ccgtacaggc ggtggttcac cataacgaaa
     8521 cggctgatcg ggcatatgaa ctctatctga gtgaaaaaca gtaactgcgg atttaaggag
     8581 aagaattatg cacgtcacac tggttgaaat taacgttcat gaagacaagg ttgacgagtt
     8641 tatcgaagtt tttcgccaga accacctggg ctctgtacag gaagaaggca atttgcgctt
     8701 cgatgtctta caggacccgg aagtgaattc gcgcttttat atctacgaag cctataaaga
     8761 tgaagacgca gtggcgttcc ataaaaccac gccccactac aaaacctgtg tcgcgaaact
     8821 ggaatcttta atgaccgggc cgcgtaaaaa acgtctgttc aatgggttga tgccgtgagg
     8881 cgaatttatc agttttatct acaattgggg taacgcgctg acgggagtaa ataaatgtct
     8941 gactggaacc cctctttata tctgcacttt gccgctgaac gatcgcgtcc ggcggtggag
     9001 ctgcttgcca gagtgccgct ggagaatgtc gattatgttg ccgatcttgg ctgtggccca
     9061 ggtaacagca ccgccctgct gcatcaacgt tggcctgctg ccaggataac gggcatcgac
     9121 tcgtctccgg cgatgattgc tgaagcgcgc agtgctttgc cagactgcca gttcgtggaa
     9181 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     9241 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     9301 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     9361 cgcgaagttg cctgggaaca aaactacccg gatcgcgggc gtgagtcgtt ggctggtgtt
     9421 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccacc
     9481 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     9541 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     9601 cagatgctga aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     9661 cgtctgttta ttgttgcccg ccgtacggag taaatatcac gtcagttggt aatggcgatc
     9721 gggaagaact tttgctggaa tttcggcttc atcgttcatc tgtaagaggt caatttcaat
     9781 agcgttacag atggcatcca gcggtaaatc attgttttca gtaccgaacg gatcttccag
     9841 ttcttccgcc agacaatcca gcgaaataaa agtataggaa atcagcacag agataaaagg
     9901 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     9961 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcgca
    10021 gcctgccaga accgctgaaa tatcgttaag ccgatcgttg aggctaataa acaggatatc
    10081 tgaaagttgt ccattgcgtc gctgaaccgc caaccactcc cccattatta acaagatgcg
    10141 gttagctgga gagttcgaag ccagtacacg ctgaagatct tcagtcttga gataatgagc
    10201 cagcacttcc acctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
    10261 ttgcagccgg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctcgcg
    10321 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt ttgcgcgctt caacgtaacg
    10381 ggcgtaaccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
    10441 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaaaataac
    10501 agcgatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
    10561 atgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
    10621 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacactactt
    10681 acaaattacc tacaaaatta caacattaac aggtgctgca tgtggcacct gttaatgata
    10741 aaggcttata ggatgttgta actaatataa tgaaataaga aacccggtcg aaaccgggtt
    10801 cagagtatta gcacaacgga cgtacagctt cgcgcatccc tttttcgaga atcgcatcca
    10861 ggtcattagc cacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
    10921 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
    10981 gactccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
    11041 ctccattacg ctcaccgcga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
    11101 ccggaagtgt gccgtttgcc ttctgccctg ccagcagctg cggcaggatg cgggtacgga
    11161 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
    11221 aacgcccggt gactgcactg gcgaaagatt ccagttcatc gcgtggcaaa tccagtaccg
    11281 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
    11341 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccaacgcg gtgtgtgcac
    11401 cgttgagtat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
    11461 tgagcggata tttgtccaga cgcagctcgg tcgctaagga tttcggtccc tgaatcacaa
    11521 acaagtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
    11581 gtttagccac ttcatcacgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
    11641 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
    11701 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc gatcaactca cacgggatga
    11761 tgatccaacc tttatccagc gcaccgttga aatggctaaa acgttcgaac agcagacggg
    11821 tcagttttgc cggataactt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
    11881 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
    11941 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaacagag cgaatcagac
    12001 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
    12061 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacac
    12121 cagaattcag atcggtgtgc tcattcagga gatctatttg ccagtcaaca aaggcgcgca
    12181 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
    12241 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
    12301 ggtacgacag gttagcaaac tttaatacga cgaacccctg ttttgatcaa ctcctgatga
    12361 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
    12421 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
    12481 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
    12541 ttcgagcgat acagacaggt atcggcttcg accatcaatt tattaaagtc atcggtcagc
    12601 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
    12661 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
    12721 ccatcgacag gattcactga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
    12781 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
    12841 tcgccacatt catgtccata gttatcgtta atacttttga aatagtcgat atcaagtagc
    12901 atgacagtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
    12961 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
    13021 agcgaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
    13081 ataccgagac gtgcggagaa catttgcgga atggagaacg gcgaaccgac cgagatatca
    13141 atcaccgaat tagccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
    13201 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
    13261 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc
    13321 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
    13381 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
    13441 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
    13501 gcaatcagac agtaattaaa tagccgtaag gcacttactg gctcatattt atttttcccc
    13561 aaacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
    13621 ttgatagcca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
    13681 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
    13741 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
    13801 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
    13861 ccttcctgga acacacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
    13921 caattgcggg tggcaattat ctcaacgtaa taagtaaatt ctcataaagg atagcatgtt
    13981 cgctgacagc ggcggcaaat aaaggtaccc tgggaatgct attttgtcgt tttccgcaat
    14041 tctcatggct gattcatctg tacaaccagc cagtgataga ttaccgccac gatataacgt
    14101 tgttgttctt cgcttagcga aacaccatgc gggatgttat gccatttagc cagacaaccg
    14161 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
    14221 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
    14281 tgttgttcga tgactggcgc gcctttctcc agacaatact gacgctcttt catgcccaaa
    14341 cgaaagcgag atcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
    14401 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
    14461 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
    14521 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
    14581 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgtgcct
    14641 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
    14701 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aaatagtttt
    14761 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
    14821 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg taggaaatat
    14881 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttaatc
    14941 gcccttgcaa catatcgcag accaccagcg caccagcatt aataaacgga ttacgcggaa
    15001 taccctgctc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
    15061 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
    15121 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
    15181 cgtcaacggt acagatagca atgcccaatc gggaaccgtc tactgtagcc agcgccggaa
    15241 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
    15301 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
    15361 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac accgtctgga
    15421 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
    15481 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
    15541 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
    15601 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
    15661 tgattgccgc aacggggcca aacatttctt cccgaaaagc ggtcatttct ggggtaacat
    15721 tcgccagaac cgttggcgga tagtagttac ctgccccagc catcttttcc ccgcccagta
    15781 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
    15841 cgaaacgagc cattggtccg agagcgttct cttcgtcacg gggatcgtcc attttcaagg
    15901 ctgccgcagc tgccacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
    15961 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
    16021 taactgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
    16081 ccagtacgca ttttttcagt gccgctccag cctgtgcacc aatagccgct ccagcacgaa
    16141 cacttccggt caccgtgata gcagcaatgc gcgagtcttt aatcatctga gtgacaccgt
    16201 cattgtcggc attcagccag ccatatacgc cttgtgggat tcccgcatct ttaaacacct
    16261 gggcaatgag ctgagcacag cccatcacat tcggcgcatg tttaagtaag tatccgttac
    16321 cagcaagaat gatgggaaca gcgccacgca tcacctgcca taacggaaaa ttccacggca
    16381 taatcgccag aatcgtcccc aacggtcgat actcaataac tgcctgctga ttttccacca
    16441 gcgtaggttc cgccttcagc attgccggac catgttctgc ataccagtca cacaaattcg
    16501 ccgatttcgc cacttcagcg cgcgcctggt tgattggttt gcccatttcg cgggtgatca
    16561 tttgcgccat ttcttcgcta cgggcgcgca gagccttacc gatatcacgc agtttttcag
    16621 cacgataatc tatatttgtc tcgcgccagt cacgaaagcc tgctgccgcc agctgaagtg
    16681 cgttttcgat atcatcagcg ccagcccacg gcaacacaga aagttgttca cccgtggcag
    16741 gatttatcga aattgcatga gttgccggag taatggtcat cggggtatct cctttatgag
    16801 tcatggtatg aagatacgca gatttactct tgctttaaaa tgaataatat taagccactt
    16861 attcacgaat cgagaatatt atggatctga cccaactgga gatgttcaac gcggttgccg
    16921 aagctggcag cattacgcag gctgcagcaa tagtgcatcg cgtgccgtcg aatctcacta
    16981 cccgtctacg ccagctggaa acagaactgg gggtagatct gtttattcgc gagaatcagc
    17041 gtttacgtct atctcctgcc gggcataact ttttacgcta tagccaacaa attctcgcgt
    17101 tagtggatga agcgcggagc gttgtcgctg gcgatgagcc gcaaggttta ttttctctgg
    17161 gttcgctgga aagcaccgca gcagttcgca ttccggccac gctggcggaa ttcaaccgtc
    17221 gttatcccaa aattcagttt tcactttcca ccggcccttc cggcactatg ctggaaggtg
    17281 tgctggaggg aaaacttaat gcggcgttta ttgatggacc cattaaccat actgccattg
    17341 acgggatacc ggtataccgc gaggagctga tgatcgtcac gccacaaggt catgcgccag
    17401 taatccgtgc cagtcaggtt aatggcagta acatttatgc cttccgcgcc aattgttcgt
    17461 atcgtcgcca cttcgagagc tggtttcatg ctgacggtgc cgctccggga actatccatg
    17521 agatggagtc ttatcacgga atgttggcct gtgtggtcgc aggagcaggt attgcgctta
    17581 ttccgcgctc tatgctggaa agtatgccgg ggcatcacca ggttgaagcg tggccgttag
    17641 ctgagcaatg gtgttggcta acaacctggc tggtctggcg tcgtggtgcg aaaacgcgtc
    17701 cgctcgaggc atttattcaa ctactggatg tgcctgactc gacaaaacag ggttaccaat
    17761 gagctatttt tgatagttct tgcggctaat atgctctata tagtgatgtt ccgatgactt
    17821 atgactatat ggggcaaata tggttacgcc agtaagcatc agcaattaca tatctcttcc
    17881 tgatgatttt cccgttcgta atattgcacc gcaggtaaaa gaagttttaa aggattttat
    17941 tgatgcactt agtacaataa tatgtaatga agagtggcgc acgtctttaa acatcaactc
    18001 agccacaaaa aagatattta ataatcttga caacttatca tatattcaga ggacttcttt
    18061 tcggggtaac gacacgctat acaatgaaaa ggttcagttt aaactcactt atcccgccag
    18121 gaatggaaga cacaaagaaa atattgaatt tcaggtagta ataaatttaa gccctattta
    18181 tttagataat ttccgccatg atggagaaat taatattttt tgcgctccca acccaaagcc
    18241 tgttactatg gggcgcgtat ttcagaccgg cgtcgagcgt gtgctctttc tgtttctgaa
    18301 tgattttatt gaacaattcc caatgatcaa ccctggcgtt cccatcaaaa gagcgcatac
    18361 accacatatt gaacccctgc ctccggatca ccataccgct gcagattact tacgccagtt
    18421 tgatttgctt gtcctgaatt ttatctctcg cggtaatttt gtcatactcc cccgattatg
    18481 gaataactct gaggttcaca gatggttcgt caataaagat cctaacttga tcaccgccat
    18541 tctcgacata acggacagcg aattaaaaga ggatttattg caaagcctga tggattcatt
    18601 aggttctaac aaacatgtac taccctaagt ctgcatctgt tttttatccc ttttagcaga
    18661 acaagaatct cctcattttc aggacttgtt tttatttttc gccaatatgt tgttacacta
    18721 tcaccaattt atgaatccca atgaaagtga tttgaatgac gtgttgatgc cagcatcatt
    18781 aagtgatgat aaaattatca aacatatggc gcgtaagacc ctcaaactgt ttgtaaaaaa
    18841 tgaaacaccg ccaaaagtta ctcatgaaga tctggtgaaa aacaggcctc gctcccctgt
    18901 cagaccgcct atacccgcaa cctccaaaac gccagacatt cctgatcgtc attaaaccgt
    18961 gatgtttacc atctctctga cgcgtgaaag aatcagcgtc agagaaacgg aaaacgcgat
    19021 ccagatcaca aatgcattgt attcacatca ttaaccgttt taagatcatt tcatcacttt
    19081 ttcgcaactc acccgataat ctgttatgac aacaaacact gtttcccgca aagtggcgtg
    19141 gctacgggtc gttacgctgg cagtcgccgc cttcatcttc aacaccaccg aatttgtccc
    19201 tgttggcctg ctctctgaca ttgcgcaaag ttttcacatg caaaccgctc aggtcggcat
    19261 catgttgacc atttacgcat gggtagtagc gctaatgtca ttgcctttta tgttaatgac
    19321 cagccaggtt gaacggcgca aattactgat ctgcctgttt gtggtgttta ttgccagcca
    19381 cgtactgtcg tttttatcgt ggagctttac cgttctggtg atcagtcgca ttggtgtggc
    19441 ttttgcacat gcgattttct ggtcgattac ggcgtccctg gcgatccgta tggctccggc
    19501 cgggaagcga gcacaggcat tgagtttaat tgccacaggt acagcactgg caatggtatt
    19561 aggattacct ctcgggcgca ttgtggggca gtatttcggt tggcgaatga ccttcttcgc
    19621 gattggtatc ggggcgctta ttaccctttt gtgcctgatt aagttacttc ccttactgcc
    19681 cagtgagcat tccggttcat tgaaaagcct cccgctatta ttccgccgcc cggcattgat
    19741 gagcatttat ttgttaactg tggtggttgt caccgcccat tacacggcat acagctatat
    19801 tgagcctttt gtgcaaaaca ttgcgggatt cagcgccaac tttgccacgg cattactgtt
    19861 attactcggt ggtgcgggca ttattggcag cgtgattttc ggtaaactgg gtaatcaata
    19921 tgcgtctgcg ttggtaagta cggcgattgc gctgttgctg gtgtgcctgg cactgctgct
    19981 acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt attttctggg ggatcgcgat
    20041 gatgatcatc gggcttggta tgcaggttaa agtgctggcg ctggcaccag atgccaccga
    20101 cgtcgcgatg gcgctattct ccgggatatt taatattgga atcggggcgg gtgcgttggt
    20161 aggtaatcag gtgagtctgc actggtcaat gtcgatgatt ggttatgtgg gcacggtgcc
    20221 tgcttttgcc gcgttaatat ggtcaatcat tatatttcgc cgctggccag tgacactcga
    20281 agaacagacg caatagttga aaggcccatt cgggcctttt ttaatgattc gttttaatga
    20341 tttccaggat gccgttaata ataaactgta cacccataca taccagcagg aaccccatca
    20401 agcgggagat cgcttcaatg ccacccttgc ccaccagccg cataattgcg ccagagctgc
    20461 gtagacttcc ccacaaaata accgccacca ggaaaaagat cagcggcggc gcaaccatca
    20521 gtacccaatc agcgaaggtt gaactctgac gcactgtgga cgccgagcta ataatcatcg
    20581 caatggttcc cggaccggcg gtacttggca ttgccagtgg tacaaaagca atattggcgc
    20641 tgggttcatc ttccagctct tccgacttgc ttttcgcctc cggtgaatca atcgctttct
    20701 gttgcggaaa aagcatccga aaaccgataa acgcgacgat taagccgcct gcaattcgca
    20761 gaccgggaat cgaaatgcca aatgtatcca tcaccagttg cccggcgtaa tacgccacca
    20821 tcatgatggc aaagacgtac accgaggcca tcaacgactg acgattacgt tcggcaccgt
    20881 tcatgttgcc tgccaggcca agaaataacg cgacagttgt taatgggtta gctaacggca
    20941 gtaacaccac cagccccagg ccaattgctt taaacaaatc taacattggt ggttgttatc
    21001 ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa caaggataaa gtgtcactct
    21061 ttagctaacc ttgcatcgca ttgaccaaaa cttgaaccga tttagcaaaa cgtggcatcg
    21121 gtcaattcat tcatttgact tatacttgcc tgggcaatat tatcccctgc aactaattac
    21181 ttgccagggc aactaatgtg aaaagtacca gcgatctgtt caatgaaatt attccattgg
    21241 gtcgcttaat ccatatggtt aatcagaaga aagatcgtct gcttaacgag tatctgtctc
    21301 cgctggatat taccgcggca cagtttaagg tgctctgctc tatccgctgc gcggcgtgta
    21361 ttactccggt tgaactgaaa aaagtgttgt cggtcgacct gggagcactg acccgtatgc
    21421 tggatcgcct ggtctgtaaa ggctggatag aaaggttgcc gaacccgaat gataagcgcg
    21481 gcgtactggt aaaacttacc accagcggcg cggcaatatg tgaacaatgc catcaattag
    21541 ttggccagga cctgcatcaa gaattaacaa aaaacctgac ggcggacgaa gtggcaacac
    21601 ttgagcattt gcttaagaaa gtcctgccgt aaacaaaaaa gaggtatgac gatgtccaga
    21661 cgcaatactg acgctattac cattcatagc attttggact ggatcgagga caacctggaa
    21721 tcaccactgt cactggagaa agtgtcagag cgttcgggtt actctaaatg gcacctgcaa
    21781 cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgcag ccgtaagatg
    21841 acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct ggcagaacga
    21901 tatggcttcg agtcgcaaca aactctgacc cgaaccttca aaaattactt tgatgttccg
    21961 ccgcataaat accggatgac caatatgcag ggcgaatcgc gctttttaca tccattaaac
    22021 cattacaaca gctagttgaa aacgtgacaa cgtcactgag gcaattatga aaccgctttt
    22081 atccgcaata gcagctgcgc ttattctctt ttccgcgcag ggcgttgcgg aacaaaccca
    22141 gcaaccgctc gttacttcct gtggcgatgt ggtggttgtt cccccatcgc aggaacaacc
    22201 accgttcgat ttaaatcaca tgggtacagg cagtgacaaa tcggatgcgc tgggcgtgcc
    22261 ctattataac caacaagcca tgtagtatat tcaggccccg gcatatcggg gcttgttaac
    22321 ctcttacctt caccgcttta cgccactgca aaccaaatac attgatatac agtccggcca
    22381 taatgagcac agcccctaag aattgcagac ctgttaagcg ttcatccagc agtaacgctg
    22441 cacttgccag accgactacc ggcaccagta atgataatgg tgcgacacgc catgtttcat
    22501 agcgtccaag cagcgtaccc caaatcccgt agccaactat cgtcgctaca aatgccagat
    22561 acatcaacgc cagaatggtg gtcatgtcga tagtgaccag actgtgaatc atttttccgg
    22621 agccatcgag aatgagcgag gcgacaaaaa acggaattat cgggattaat gcgctccaga
    22681 cgaccagaga cattaccgcc ggacgggatg aatgagacat gatctttttg ttgaagatat
    22741 tgccactcgc ccaactaaat gccgctgcca gagtcaacat aaagccgagc atcgccacat
    22801 gctgaccgtt aaagctatct tcgattaaca ccagtacgcc agaaatcgct aacgcaatcc
    22861 ccgccagttg cttaccgtgc agccgttcgc caaaagtaaa cgcgccaagc acgatagtaa
    22921 aaaacgcctg agcctgtaag accagcgagg ccagtccagc cggcataccg aagttaatgg
    22981 cgcagaagag aaaggcaaac tgtgcaaaac tgatggttaa accatacccc agcagtaaat
    23041 tcagcggtac ttttggtcgt gccacgaaaa agattgccgg gaaggcgacc agcataaagc
    23101 gcaaaccggc cagcatcagc ggtggcatgt tatgaagccc cactttgatg accacaaaat
    23161 taagccccca tacaacgacg actagtagcg ccaacacccc atcttttcgc gacattctac
    23221 cgcctctgaa tttcatcttt tgtaagcaat caacttagct gaatttactt ttctttaaca
    23281 gttgattcgt tagtcgccgg ttacgacggc attaatgcgc aaataagtcg ctatacttcg
    23341 gatttttgcc atgctatttc tttacatctc taaaacaaaa cataacgaaa cgcactgccg
    23401 gacagacaaa tgaacttatc cctacgacgc tctaccagcg cccttcttgc ctcgtcgttg
    23461 ttattaacca tcggacgcgg cgccacgctg ccatttatga ccatttactt gagtcgccag
    23521 tacagcctga gtgtcgatct aatcggttat gcgatgacaa ttgcgctcac tattggcgtc
    23581 gtttttagcc tcggttttgg tatcctggcg gataagttcg acaagaaacg ctatatgtta
    23641 ctggcaatta ccgccttcgc cagcggtttt attgccattc ctttagtgaa taacgtgacg
    23701 ctggtggtgc tcttttttgc cctcattaac tgcgcctatt ccgtttttgc taccgtgctg
    23761 aaagcctggt ttgccgacaa tctttcatcc accagcaaaa cgaagatctt ctcgatcaac
    23821 tacaccatgc tcaatattgg ctggaccgtc ggcccgccac tcggcacgct gttggtcatg
    23881 cagagcatca atctgccctt ttggctggcc gctatctgct ctgcgtttcc catgtttttc
    23941 attcaaattt gggtaaagcg cagcgagaaa atcatcgcca cggaaacagg cagtgtctgg
    24001 tcgccgaaag ttttattaca agataaggca ctgttgtggt ttacctgctc tggctttctg
    24061 gcttcttttg ttagtggcgc atttgcttca tgcatttcac aatatgtgat ggtgattgct
    24121 gatggtgatt ttgccgaaaa ggtggtcgcg gttgttcttc cagtgaatgc tgccatggtg
    24181 gttacgttgc aatattccgt gggccgccgc cttaacccgg ctaacatccg cgcgctgatg
    24241 acagcaggca ccctctgttt cgtcatcggt ctgatcggtt ttattttttc cggcaacagc
    24301 ctgctattgt ggggtatgtc agctgcggta tttactgtcg gtgaaataat ttatgcgccg
    24361 ggcgagtata tgttgattga ccatattgcg ccgccaggaa tgaaagccag ctatttttcc
    24421 gcccagtcat taggctggtt gggcgcagcg gtaaatccat tagtgagtgg tgttgtactt
    24481 accactctcc cgtcgtcttc gctgtttatc attctggcgt tagtcatcat tgccgcgtgg
    24541 gcgctgatgt taaaaggtat taacgccaga ccccagaggc aaccagcact ctgttgacgt
    24601 tcgtgccgtt ccactactgg cacggcttcg ctaatcatta ttctgaggca aaggcatagc
    24661 cttcatcttc ccagccggtt atcccgccga gcatgatttt aaccggcttt ccgagtcgag
    24721 ccagtttgag tgccgcacgg tctgcaccgt tacaatgtgg ccccgcgcaa tacaccacaa
    24781 atagcgtatc gtcgggccat tcggccattc tttctgcggt catttgctta tggggtaaat
    24841 ggagagcgcc tgggatatgc cgacgcgcga agtcctcttc acgtccaacc acatgaagta
    24901 aaacgaaatc atcgccaata taagccagag agtcatgaac atcggcgcaa tctgtttcga
    24961 cactcaagcg cgcgagaaaa tgcgcctcag caacttctgg cttcgctgtg gtaaattcag
    25021 tgacataacg catattgctt tgtccttttg gtttgttgtg ttaacaataa tgtaactaat
    25081 ctacctttca ctgaccgcct ctcgatatgc cagaaatgag caaaatgatg acaacgttac
    25141 aacgtccgct ggtcattgca ctggcatatg acgggctatg tacttttgaa tttggcgttg
    25201 ccgttgaaat attcggcctg ccacgaccgg aactgggcga taactggtat cgttttgctg
    25261 ttgctgcggt agaaccaggt gcgctacaag ccaccggggg gatccacgtg gttgctgatg
    25321 gtggactgga gttattgccc aacgccgata tgattattat tcctggctgg cgcggaatag
    25381 atgccaaagt gccggagatg ctatgccagt cattgcgcga ggcacaccag cgcggtactc
    25441 gcctggtatc aatatgctct ggcgtgtttg tccttgctgc tgcgggcctt cttcatcagc
    25501 gaaaagcaac cacccactgg cgctacactg aacaactcca gcaccgttac ccttcaatca
    25561 gcgtaagtga caatgtgtta tatcaggatg aaggacaagt gatgacctcc gccggaagcg
    25621 ccgcggggat cgatctgtgt ttacatatcg tgcgcaaaga ttttggtcat gagattgcta
    25681 ataatgtcgc ccggcgactg gtcattcagc cacatcgcca gggggatcaa ccacaaaatc
    25741 tgacgcgccc aatggcctca ccgcgtgaaa gccagacgtt aggtgcctta ttcgactttt
    25801 tgcagcagaa tctggcacaa acacatactg tgtcttccct cgccgaacgg gtaaatatga
    25861 gtccgcgcac ttttttaaga cgttttgagg cgttaactgg catgacgccc gcccgctggc
    25921 tgctcaatga gcgcttaata cgagtacagg attatctgca aaattcggtt tttaacatcg
    25981 agaaaattgc cgagctgacg ggttttggca attcagcgtt attgcgtcat cactttcacc
    26041 aacgtttttt attatctcct tcgcaatatc gcaaaaacag ccgttcccca tcacaataat
    26101 cgcctgattc agcctttgtt tagataacct ctgctaatat ctttctcatt gagataaaaa
    26161 ttaaggtaag cgaggaaaca caccacacca taaacggagg caaataatgc tgggtaatat
    26221 gaatgttttt atggccatac tgggaataat tttattttct ggttttctgg ccgcgtattt
    26281 cagccacaaa tgggatgact aatgaacgga gataatccct cacctaaccg gccccttgtt
    26341 acagttgtgt acaaggggcc tgatttttat gacggcgaaa aaaaaccgcc agtaaaccgg
    26401 cggtgaatgc ttgcatggat agatttgtgt tttgctttta cgctaacagg cattttcctg
    26461 cactgataac ggatcgttga cacagtagca tcagttttct caatgaatgt taaacggagc
    26521 ttaaactcgg ttaatcacat tttgttcgtc aataaacatg cagcgatttc ttccggtttg
    26581 cttaccctca tacattgccc ggtctgctct tccaatgacc acatccagag tctcttcagg
    26641 aaatgcgcga ctcacacctg ctgtcacggt aatgttgata tgcccttcag aatgtgtgat
    26701 ggcatggtta tcgactaact ggcaaattct gacacctgca cgacatgctt cttcatcatt
    26761 agccgctttg acaataatga taaattcttc gcccccgtag cgataaaccg tttcgtaatc
    26821 acgcgtccaa ctggctaagt aagttgccag ggtgcgtaat actacatcgc cgattaaatg
    26881 cccgtaggta tcattaacca atttaaatcg gtcaatatcc aacaacatta aataaagatt
    26941 cagaggctca gtgttgcgta actgatgatc aaaggattca tcaagaaccc gacgccccgg
    27001 caatcccgtc aaaacatcca tattgctacg gatcgtcagc aaataaattt tgtaatcggt
    27061 taatgccgca gtaaaagaaa gcaccccctc ctgaaaggcg tcgaaatgcg cgtcctgcca
    27121 gtgattttca acaatagcca gcattaattc ccgaccacaa ttatgcatat gttgatgggc
    27181 agaatccatt agccgaacgt aaggtaattc atcgttatcg agtggcctca gatgatcaat
    27241 ccaccgacca aactggcaca gtccataaga atggttatcc gttatttctg gcttactggc
    27301 atctctcgcg accacgctgt gaaacatact caccagccac tgatagtggg catcgatagc
    27361 cttattgaga tttaacaaga tggcatcaat ttccgttgtc ttcttgatca ttgccactcc
    27421 tttttcacag ttccttgtgc gcgctattct aacgagagaa aagcaaaatt acgtcaatat
    27481 tttcatagaa atccgaagtt atgattcatc tctgagataa cattgtgatt taaaacaaaa
    27541 tcagcggata aaaaagtgtt taattctgta aattacctct tcattatcgc aaatgaaagg
    27601 atgacaaata gcataaccca gtacccaaat ggcccggtag ttcaggccat caggctaatt
    27661 tatttttatt tctgcaaatg agtgacccga acgacagccg gcgcgctttt cttatccaga
    27721 ctgccgctaa tgttgatcat ctggtccggc tgaacttctc gtccatcaaa aacggtcgca
    27781 ggaataacaa cattaatttc accactctta tcgcgaaaaa cgtaacggtc ctctcctttg
    27841 tgagaaatca aattaccgcg tagtgaaacc gaagcgccat cgtgcatggt ttttgcgaaa
    27901 tcaacagtca ttttttttgc atcatcggtt ccgcgatagc catcttctat tgcatgaggc
    27961 ggcggtggcg ctgcatcctg ttttaaaccg ccctggtcat ctgccaacgc ataaggtatg
    28021 acaagaaaac ttgctaatac aatggcctga aatttcatac taactcctta attgcgtttg
    28081 gtttgactta ttaagtctgg ttgctatttt tataattgcc aaataagaat actgccaatt
    28141 gttataaggc atttaaaatc agccaactag ctgtcaaata ttcagagaat ttaactcact
    28201 aaagttaaga agattgaaga gtcttaaaca tattttcaga ataatcggat ttatatgttt
    28261 gaaaattatt atattggacg agcatacaga aaaagcaaat cacctttaca tataaaagcg
    28321 tggacaaaaa acagtgaaca ttaatagaaa taaaattgta caacttgcag ataccgacac
    28381 tattgaaaac ctgacatccg cgttgagtca aagacttatc gcggatcaat tacgcttaac
    28441 taccgccgaa tcatgcaccg gcggtaagtt ggctagcgcc ctgtgtgcag ctgaagatac
    28501 acccaaattt tacggtgcag gctttgttac tttcaccgat caggcaaaga tgaaaatcct
    28561 cagcgtaagc cagcaatctc ttgaacgata ttctgcggtg agtgagaaag tggcagcaga
    28621 aatggcaacc ggtgccatag agcgtgcgga tgctgatgtc agtattgcca ttaccggcta
    28681 cggcggaccg gagggcggtg aagatggtac gccagcgggt accgtctggt tcgcgtggca
    28741 tattaaaggc cagaactaca ctgcggttat gcattttgct ggcgactgcg aaacggtatt
    28801 agctttagcg gtgaggtttg ccctcgccca gctgctgcag ttactgctat aaccaggctg
    28861 gcctggcgat atctccagga cagccattgg tggtgtttat atgttcaagc cacgatgttg
    28921 cagcatcggc ataatctgag gagccttacc gcgccattgt cgatacaggc gttccagatc
    28981 ttcgctgtta cctctggaaa ggatcgcctc gcgaaaacgc agcccatttt cacgcgttaa
    29041 tccgccctgc tcaacaaacc actggtaacc atcatcggcc aacatttgcg tccacagata
    29101 agcgtaataa cctgcggcat atccgccacc aaaaatatgg gcaaaataac tgctgcgata
    29161 gcgtggcggt atagcaggaa gatccatatt ttctgccacc agcgcccgca attcaaaatc
    29221 atcgacatcc tgcattgctt cgttttcttc caggcaatgc cagcgcatat cgagaagtgc
    29281 ggcgctaagc agttcgctca tctcataccc tttgttgaac aggctggcat tacgcatttt
    29341 ctgttgcagt tcgtcaggca ttgctgcccc gctctgataa tgccgggcgt agcgagcgaa
    29401 tacctgcgga tgcgttgccc agtgttcgtt gatttgcgac ggaaattcga caaaatcacg
    29461 cggcgtgttg gtgccggaga gcgtggcata acgctggcgg gcaaaaaggc cgtgcagcgt
    29521 atgaccaaat tcatggaata aggttatgac atcatcccag agtaacaacg caggctcacc
    29581 ggcagcgggt ttctgataat tgcagacgtt ataaattacc ggatgcgttt cattaagcgt
    29641 tgattgctca acaaaattgc ccatccatgc accgccgctt tttgaatcac gggcgaagaa
    29701 atcaccgtaa aataacgcca gccccacgcc attatgatca aaaatttccc acacccgaac
    29761 gtcaggatgg tagacaggaa tatcaaaacg ttcgacaaac ttaataccga agagctgatt
    29821 cgcggtccag aatacacctt cattcaacac cgtgtttaat tcaaaatatg gcttgagctg
    29881 cgcctcatca agatcaaatt tctctcgccg gacctgttcg gcataaaatg cccagtccca
    29941 cggctgcgcg ctaaacccgc cctgctgctt atcaataacc gcctgtatgg aggctaattc
    30001 atcgctcgca cgttgacgcg ccgctggaac aatttcccgc ataaagttga gtgctgcttc
    30061 aggtgttttt gccatctgat cggcgatttt ccatgcggca taatgaggaa aaccgagtaa
    30121 tgtagcctgt tgtgcacgga tctccaccag acgttgaatg atagcgcggg tatcattggc
    30181 atcatttttt tccgctcgcg tccagcccgc aataaacagt ttttcacgcg tcgcacgatc
    30241 gcgcatttcg gcaagcgccg gttgttgggt ggtattcagc agcggaatca gccatttgtt
    30301 atccagacct ttctcgcgag ccgcctctgc cgccagcgca atctcttgct cactcattcc
    30361 tgccagctgc gcgatatcgt tcacaaccag accgccggat ttatttgctg ccagtaaccg
    30421 ctggttaaac tgactggtca gtgtcgcagc ttctgtattc agtactttta attttgcttt
    30481 atcagcttgt gcaagtttgg ctccggctag gacaaaacgt tgatgaatca cctccaccag
    30541 gcggatggat tcactatcaa gccccagaga ttcacggcgt tgccagacag catctacccg
    30601 cgcgaataat tcaccgttca gatagatatc attagccagt tccgccagtt cagcggaaaa
    30661 ctgctcgtca agacgctgta attcatcatt ggtatgcgcc gcagtcatcg caaaaaagac
    30721 gctggtaacg cgggtaagta attctccgct ttgttccagt gccagaatag tattgttgaa
    30781 atcaggtgtt tgcgggttaa gcgcgatggc agcaatttct gcccgctttt gctgtattcc
    30841 ctcatcgaat gccgggcgat agtgatgatt ggcaatttga tcaaaatgag gagccagata
    30901 cggcagtgtg ctttgcacaa ggaaaggatt aattgttgtc attttcttct cctgaacgcg
    30961 aggtgttcca tagcgtaggc ttactgataa cggagtgcaa tcttgcaaac cagtattacc
    31021 cgctcttaag catcacgtgc tatgttattg acacacaaaa gcgttgagga acagtgagat
    31081 gatcgtttta gtaactggag caacggcagg ttttggtgaa tgcattactc gtcgttttat
    31141 tcaacaaggg cataaagtta tcgccactgg ccgtcgccag gaacggttgc aggagttaaa
    31201 agacgaactg ggagataatc tgtacatcgc ccaactggac gttcgcaacc gcgccgctat
    31261 tgaagagatg ctggcatcgc ttcctgccga gtggtgcaat attgatatcc tggtaaataa
    31321 tgccggtctg gcgttgggca tggagcctgc gcataaagcc agcattgaag actgggaaac
    31381 gatgattgat accaacaaca aaggcctggt atatatgacg cgcgccgtct taccgggtat
    31441 ggttgaacgt aatcatggtc atattattaa cattggctca acggcaggta gctggccgta
    31501 tgccggtggt aacgtttacg gtgcgacgaa agcgtttgtt cgtcagttta gcctgaatct
    31561 gcgtacggac ctgcatggta cggcggtgcg cgtcaccgac atcgaaccgg gtctggtggg
    31621 tggcaccgag ttttccaatg tccgctttaa aggcgatgac ggtaaagcgg aaaaaaccta
    31681 tcaaaatacc gttgcattaa cgccagaaga tgtcagcgaa gccgtctggt gggtgtcaac
    31741 gctgcctgct cacgtcaata tcaataccct ggaaatgatg ccggttaccc aaagctatgc
    31801 cgggctgaat gtccaccgtc agtaattttt atacccggtg taactgccgg gttattgctt
    31861 gtcacaaaaa agtggtagac tcatgcagtt aactcactca caagcaagaa cgaatgaccg
    31921 tcgaaacgca acttaatccc acacagcctg tcaatcagca gatttatcgt attcttcgtc
    31981 gcgacattgt ccattgcctg attgcaccag gcacaccgtt gtcggaaaaa gaagtttctg
    32041 ttcgtttcaa tgtgtcacgc cagccggttc gtgaagcctt tattaaactg gcggaaaacg
    32101 gcctgattca aattcgtccg cagcgtggca gctacgtcaa caaaatttcc atggcccagg
    32161 tgcgcaacgg cagttttatc cgtcaggcca ttgagtgcgc ggtggcgcgt cgggcggcga
    32221 gtatgattac cgaaagccag tgctaccaac tggaacaaaa tcttcaccag caacgcattg
    32281 ccattgagcg caagcaactg gatgattttt ttgaacttga tgacaacttc catcaactcc
    32341 tgacgcagat tgccgactgt caactggcgt gggataccat tgagaacctg aaagcgaccg
    32401 ttgatcgcgt gcgctatatg agtttcgacc atgtttctcc accagaaatg ctgttacgcc
    32461 agcatcttga tatattctct gccctgcaaa aacgtgatgg cgatgcggta gaacgtgcaa
    32521 tgacgcaaca tttgcaggaa atcagcgaat ccgtgcgcca gatccgccag gaaaatagcg
    32581 actggtttag cgaagagtaa ttcatttcct ctcatcccat ccggggtgag agtcttttcc
    32641 cccgccttat ggctcatgca tgcatcaaaa aagatgtgag cttgatcaaa aacaaaaaat
    32701 atttcactcg acaggagtat ttatattgcg cccgttacgt gggcttcgac tgtaaatcag
    32761 aaaggagaaa acacctatga cgacctacga tcgtaaccgt aacgcaatca ccactggcag
    32821 ccgtgttatg gttagcggca ccggtcacac tggcaagatc ctgtcgattg atactgaagg
    32881 tctgaccgct gagcaaatcc gccgcggaaa aaccgtagtt gttgaaggtt gtgaagagaa
    32941 actggcacca ctggacctga ttcgtctcgg catgaactaa gcgtgtgaat gccgccgatg
    33001 gcggcattgc tttttttact tcacggaata tttcgccacg gtcgctttcg caccatgcgc
    33061 taataaagac aagtacgctt ccgtcacttt tgcagtaaac aagctattgt ctggcaaatc
    33121 accaccaaaa atcgccttaa tcgccagcaa tgactggacg cgcgctttcc cttcggcact
    33181 actttgtaca gccttctgaa taacaggtaa cagtgggtca ctgatttcta tcggatttcc
    33241 ctgttcatca acaccaccga cataacgcat ccaacccgcg acgcccagcg ccagcagatc
    33301 gaacttgctg tcatgcgcca gatgccagcg aacagaatcc aacatccgct gtggcaattt
    33361 ctggctgcca tccatcgcaa tctgccaggt tcgatgacgt aacgccgggt tgctatagcg
    33421 tgcaattaat cggttagcgt aatcttgcaa atcaacgccc tgcactttca gcgtcggcgc
    33481 ttgttcctgc aacatcaagg catacgccgc atgacgataa tgttcatctt ccatacagtc
    33541 attaatgtgc tgatatccgg caagataacc cagatacgcc aggaatgaat gactgccgtt
    33601 gagcatgcgc aacttcatct cttcataagg cagcacatca ctaaccagtt cggctcccgc
    33661 tttttcccat tccggacgtc cggcaacaaa gttatcttcg attacccact ggcggaaagg
    33721 ttcacaggca acgccagcag gatcgcgcac accggtaagc tgttcgattt tcgccagcgt
    33781 atcctctgtc actgcgggca caatacggtc caccattgtt gatgggaaag tcacgttatc
    33841 ttcgatccat tgtgctagtt ttacatcaac ggcttgtgcg taggaagtga caacgtcacg
    33901 cataacatga ccgttttctg gcatgttgtc acatgacatg acggtaaatg cgggaagtcc
    33961 tgccgcttta cggcgagcca gcgcctcaac aatcacccct gttgctgttt tcggctggtg
    34021 gggattttgc acgtcggcag cgaccatcgg gtgatcgagc attaactgtc cggtcgccgg
    34081 agagtggaaa tacccttttt cggtgattgt cagagagaca atcgcgattt gcggttcaca
    34141 catcgcagcc aacacggttt ctaagccatc tatctgtacg tgcaaggctt ttttaacgac
    34201 gccaacgacg cgagccgtcc acgcatcggc cgacatttcc gcaacggtat aaagattatc
    34261 ttgctgatgt aaatcggcaa tttgctgttc gccgccgatt aagttgacct cataatatcc
    34321 ccagtcactg aaatgttctg tagcaagaat atcggcatac acgccctgat gcgcacggtg
    34381 aaatgcacca aagcctaaat gaacaattct tggggccagg ttattacgat cataaacagg
    34441 gagtgtcgct tttgctgata acaaattatt tcccataaca attccttaaa tttaaatatg
    34501 gcaagctaca tgttttgtta tatgaataaa aatcccctct ccggtaagag aagggattga
    34561 ggatttacag acttctggaa ggttgcgcag ctcttacaac acgcggttga tcttccgcag
    34621 cgtcttccag cgcacttaaa tcacggtctt tcacttctgg cattttcagc gcagagatta
    34681 aaccaatcac tgaatatgcc atgatcataa tggcgatcgg ataccaggat tccgtcatgg
    34741 tgcagaaaat acccgccagg ataggaccaa aaccggaagc gataagacca ccaatttctt
    34801 tagaaatagc catccgggta aagcggtttt tacagccgaa catttctgcc atggtaatgt
    34861 tttccagagc aaataatccc agcaccgcac agttatgaat cacaatcagt gcaaccataa
    34921 tggtgctcgg ggcatagctt ttatctacga tgatagaaag cattggccat gccagcacaa
    34981 tcgcggaggt attcataata atatacggga tccggcgacc aattttatcg gataaccaac
    35041 caaggaacgg aatggtcata aagccgggaa tcgaactgat catcaatgca tctgttggaa
    35101 ttgctttgtt aaacaataac gtctgtacta aatagcctgc aaggaaagtc tgaattaacc
    35161 cggagttacc tgcctgacca aaacgcagcc ctgttgccag ccagaaggat ttgctctgga
    35221 atatgctacc agcaggtgca ggttttgctg tcggttggtt gctgtcgtta accttctcaa
    35281 agaccgggct ttctttcaga ttcatacgca accagatagc aaagaccatc acgacaacgc
    35341 tcgccaggaa cggtatacgc catccccacg ccagcagttc ctctttactg agaatgaaga
    35401 acataaaggc ccagattgcc gttgcgctca aggttccgca gttagttccc atagccacaa
    35461 atgaggagat aattccgcgc ttaccttttg gcgcatattc cgccagcatc gtaccggcac
    35521 cggaaatttc cgcacctgca cccaatccct gaataatacg cagcgtcacc agcaagatgg
    35581 gggcaaaaac accaatctgt gcataggtcg gtaacactcc aattaaggtg gtacagatcc
    35641 ccatcatggt gatggtaata aagagcactt ttttacgccc tattctgtcg cccattttgc
    35701 cgaaaataaa tgctccgaca atacgcgcca cataacctgc accgtaggtt cccattgcca
    35761 gaattaacgc cattgccgtt gatgattcag gaaaaaatat ttcatgaaac actaacgctg
    35821 cgccgagcga atataactgg aaatccataa ttcatagatg ttttttacta ttctgtgggt
    35881 ttttgggtgt tttctaagtt ttttcagatg gttgtatttt ttctaaaaat ccctaatctc
    35941 gattttgctg tttatttgag gcctttttat gtcccatata tgccccacag ataccccgca
    36001 gccaaaatca acaaaatgcc aaaaggttct gttcctgccc tgcaacaaga aatgctgcga
    36061 cgtgtcagta aacgttatga cgatgtagaa gtgatcatca aatccaccag caacgatggc
    36121 ctttcagtta ctcgcaccgc cgataaagat tctgcaaaaa cttttgttca ggagacgctg
    36181 aaagatacct gggagtctgc tgacgagtgg tttgttcgct aattaacgag taaaatcagt
    36241 aacggctgga aatcattcaa tactcgcact atcgaaagtt caccagccaa ccgcagcacg
    36301 ttcttgcata cgaggtgccg cgttttacat gctgtaccga gaattaattt cattttacga
    36361 aaagttaacc cgccattgct atcttaattt tttaaaacga agttacctct gtcagagtca
    36421 aaagcacaat tatctcttcc tacaatcatt gaaactgcga ttgattccct gctcaaagga
    36481 tgagccgccc ttgtt
//
LOCUS       DAZFDC010000031        36279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFDC010000031 DAZFDC010000000
VERSION     DAZFDC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.031"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..1741
                     /gene="ydeN"
                     /locus_tag="ACHJ48_001712"
     CDS             59..1741
                     /gene="ydeN"
                     /locus_tag="ACHJ48_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4707288.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1793..2950
                     /gene="ydeM"
                     /locus_tag="ACHJ48_001713"
     CDS             1793..2950
                     /gene="ydeM"
                     /locus_tag="ACHJ48_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO4707289.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKLGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2916..3038)
                     /locus_tag="ACHJ48_001714"
     CDS             complement(2916..3038)
                     /locus_tag="ACHJ48_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707290.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3241..4926
                     /gene="yddA"
                     /locus_tag="ACHJ48_001715"
     CDS             3241..4926
                     /gene="yddA"
                     /locus_tag="ACHJ48_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO4707291.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGMLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            4964..7336
                     /gene="yddB"
                     /locus_tag="ACHJ48_001716"
     CDS             4964..7336
                     /gene="yddB"
                     /locus_tag="ACHJ48_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO4707292.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKFKRFNADDSSVKLGYRTTRSDWLTSHINENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGMAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7393..10176
                     /gene="pqqL"
                     /locus_tag="ACHJ48_001717"
     CDS             7393..10176
                     /gene="pqqL"
                     /locus_tag="ACHJ48_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4707293.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLLQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMEELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10537..11937
                     /gene="gadB"
                     /locus_tag="ACHJ48_001718"
     CDS             10537..11937
                     /gene="gadB"
                     /locus_tag="ACHJ48_001718"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO4707294.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            12093..13628
                     /gene="gadC"
                     /locus_tag="ACHJ48_001719"
     CDS             12093..13628
                     /gene="gadC"
                     /locus_tag="ACHJ48_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO4707295.1"
                     /translation="MATLVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            13759..15078
                     /gene="digH"
                     /locus_tag="ACHJ48_001720"
     CDS             13759..15078
                     /gene="digH"
                     /locus_tag="ACHJ48_001720"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO4707296.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            15455..16837
                     /gene="dosC"
                     /locus_tag="ACHJ48_001721"
     CDS             15455..16837
                     /gene="dosC"
                     /locus_tag="ACHJ48_001721"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO4707297.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            16862..19251
                     /gene="dosP"
                     /locus_tag="ACHJ48_001722"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             16862..19251
                     /gene="dosP"
                     /locus_tag="ACHJ48_001722"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            19509..20090
                     /gene="ddpX"
                     /locus_tag="ACHJ48_001723"
     CDS             19509..20090
                     /gene="ddpX"
                     /locus_tag="ACHJ48_001723"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO4707298.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            20104..21654
                     /gene="ddpA"
                     /locus_tag="ACHJ48_001724"
     CDS             20104..21654
                     /gene="ddpA"
                     /locus_tag="ACHJ48_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4707299.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASNDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            21656..22678
                     /gene="ddpB"
                     /locus_tag="ACHJ48_001725"
     CDS             21656..22678
                     /gene="ddpB"
                     /locus_tag="ACHJ48_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4707300.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            22675..23571
                     /gene="ddpC"
                     /locus_tag="ACHJ48_001726"
     CDS             22675..23571
                     /gene="ddpC"
                     /locus_tag="ACHJ48_001726"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO4707301.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            23568..24554
                     /gene="ddpD"
                     /locus_tag="ACHJ48_001727"
     CDS             23568..24554
                     /gene="ddpD"
                     /locus_tag="ACHJ48_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4707302.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDTVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARDSGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            24547..25473
                     /gene="ddpF"
                     /locus_tag="ACHJ48_001728"
     CDS             24547..25473
                     /gene="ddpF"
                     /locus_tag="ACHJ48_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO4707303.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMMFQDPLSSLNPRLPVWRI
                     ITEPLWIAKHSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(25529..25960)
                     /gene="osmC"
                     /locus_tag="ACHJ48_001729"
     CDS             complement(25529..25960)
                     /gene="osmC"
                     /locus_tag="ACHJ48_001729"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO4707304.1"
                     /translation="MTIHKKGQAHWESDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            26305..26520
                     /gene="bdm"
                     /locus_tag="ACHJ48_001730"
     CDS             26305..26520
                     /gene="bdm"
                     /locus_tag="ACHJ48_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO4707305.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            26622..26759
                     /gene="sra"
                     /locus_tag="ACHJ48_001731"
     CDS             26622..26759
                     /gene="sra"
                     /locus_tag="ACHJ48_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO4707306.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            26916..28613
                     /gene="maeA"
                     /locus_tag="ACHJ48_001732"
     CDS             26916..28613
                     /gene="maeA"
                     /locus_tag="ACHJ48_001732"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO4707307.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDRDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            28747..29757
                     /gene="adhP"
                     /locus_tag="ACHJ48_001733"
     CDS             28747..29757
                     /gene="adhP"
                     /locus_tag="ACHJ48_001733"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO4707308.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            29903..30187
                     /gene="yddM"
                     /locus_tag="ACHJ48_001734"
     CDS             29903..30187
                     /gene="yddM"
                     /locus_tag="ACHJ48_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO4707309.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAAKTVDVSRLRRLVAQ"
     gene            complement(30415..31068)
                     /gene="fdnI"
                     /locus_tag="ACHJ48_001735"
     CDS             complement(30415..31068)
                     /gene="fdnI"
                     /locus_tag="ACHJ48_001735"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO4707310.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(31061..31945)
                     /gene="fdnH"
                     /locus_tag="ACHJ48_001736"
     CDS             complement(31061..31945)
                     /gene="fdnH"
                     /locus_tag="ACHJ48_001736"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO4707311.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQSGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(31958..35005)
                     /gene="fdnG"
                     /locus_tag="ACHJ48_001737"
     CDS             complement(31958..35005)
                     /gene="fdnG"
                     /locus_tag="ACHJ48_001737"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(34418..34420),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4707312.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            35237..36118
                     /gene="yddG"
                     /locus_tag="ACHJ48_001738"
     CDS             35237..36118
                     /gene="yddG"
                     /locus_tag="ACHJ48_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO4707313.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         9391 a         8889 c         8819 g         9180 t
ORIGIN      
        1 acttatttct cttcgtaaaa ttactttgga attaagtaca ataagaagag gaacatttat
       61 gaagtctgca ttaaagaaaa gtgtcgtaag tacctcgata tctttgatac tggcatctgg
      121 tatggctgca tttgctgctc atgcggcaga tgatgtaaag ctgaaagcaa ccaaaacaaa
      181 cgttgctttc tcagacttta cgccgactga atacagtacc aaaggaaagc caaatattat
      241 cgtactgacc atggatgatc ttggttatgg acaacttcct tttgataagg gatcttttga
      301 cccaaaaaca atggaaaatc gtgaagttgt cgatacctac aaaataggga tagataaagc
      361 cattgaagct gcgcaaaaat caacgccgac gctcctttca ttaatggatg aaggcgtacg
      421 ttttactaac ggctatgtgg cgcacggtgt ttccggcccc tcccgcgccg caataatgac
      481 cggtcgagcg cccgcccgct ttggtgtcta ttccaatact gatgctcagg atggtattcc
      541 gctaacagaa actttcttgc ctgaattatt ccagaatcat ggttattaca ctgcagcagt
      601 aggtaaatgg cacttgtcaa aaatcagtaa tgtgccggta ccggaagata aacaaacacg
      661 tgactatcat gacaacttca ccacattttc tgcggaagaa tggcaaccgc aaaaccgtgg
      721 ctttgattac tttatgggat tccacgctgc aggaacggca tattacaact ccccttcact
      781 gttcaagaat cgtgaacgtg tccccgcaaa aggttatatc agcgatcagt taaccgatga
      841 ggcaattggc gttgttgatc gtgccaaaac acttgaccag ccttttatgc tttacctggc
      901 ttataatgct ccgcacctgc caaatgataa tcctgcaccg gagcaatatc agaagcaatt
      961 taataccggt agtcaaacag cagataacta ctacgcttcc gtttattctg ttgatcaggg
     1021 tgtaaaacgc attctcgaac aactgaagaa aaacggacag tatgacaata caattattct
     1081 ctttacctcc gataatggtg cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa
     1141 aggctataag agtcagacct atcctggcgg tactcacacc ccaatgttta tgtggtggaa
     1201 aggaaaactt caacccggta attatgacaa gctgatttcc gcaatggatt tctacccgac
     1261 agctcttgat gcagccgata tcagcattcc aaaagacctt aagctggatg gcgtttcctt
     1321 gctgccctgg ttgcaagata agaaacaagg cgagccacat aaaaatctga cctggataac
     1381 ctcttattct cactggtttg atgaggaaaa tattccattc tgggataact accacaaatt
     1441 tgtccgtcat cagtcagacg attacccgca taaccccaac actgaggact taagccaatt
     1501 ctcttatacg gtgagaaata acgattattc gcttgtctat acagtagaaa acaatcagtt
     1561 aggtctctac aaactgacgg atctacagca aaaagataac cttgccgccg ccaatccgca
     1621 ggtcgttaaa gagatgcaag gcgtggtaag agagtttatc gacagcagcc agccaccgct
     1681 tagcgaggta aatcaggaga agtttaacaa tatcaagaaa gcactaagcg aagcgaaata
     1741 actaaacctt catgcggcgg atttttccgc cgccttattg agcgagatag cgatgcacgt
     1801 tacagccaag ccctccagtt ttcaatgtaa tctcaaatgt gattactgtt tttaccttga
     1861 aaaagagtcg cagtttactc atgaaaaatg gatggatgac agcactctga aagagttcat
     1921 caaacaatat atcgcagcgt ctggcaatca ggtctatttt acctggcaag gcggtgaacc
     1981 cactctggct ggcctggatt ttttccgtaa agttattcac tatcaacaac gctatgcagg
     2041 ccaaaaacgt atttttaatg cattacaaac gaatggcatt ttattgaata atgaatggtg
     2101 tgcctttctc aaagaacatg agtttctggt tggtatctcg atcgatggac cccaggagct
     2161 acatgaccgt tacagacgca gtaattcagg taacggtact tttgcaaaag tgatagcagc
     2221 catcgagcgt ctgaaatcat atcaagtaga gtttaatacg ttaaccgtca ttaataacgt
     2281 taatgtccat taccctcttg aggtttatca ttttttaaaa tctatcggca gtaaacatat
     2341 gcaatttatc gaattgctcg aaaccgggac gccgaatatt gatttcagtg gtcatagtga
     2401 gaacacattc cgtatcattg atttttctgt gcctcccacg gcttatggca agtttatgtc
     2461 aaccattttt atgcaatggg ttaaaaacga tgtgggtgaa attttcatcc gtcagtttga
     2521 aagctttgtc agccgttttt tggggaatgg gcataccagt tgtattttcc aggagtcctg
     2581 caaggataat ctggttgttg aaagtaatgg agatatttac gaatgcgatc attttgtcta
     2641 tccacagtac aaacttggaa acattaataa atctgaactt aaaacgatga acagtgtaca
     2701 actgacagcg caaaaaaaac ggatttcagc gaaatgtcag caatgtgcat ataaacctat
     2761 ctgcaatggc ggttgcccta agcatcgtat tactaaagta aacgatgaga ctgtttccta
     2821 tttttgcgaa ggttataaaa tccttttttc aaccatggta ccttatatga acgccatggt
     2881 ggagttagcc aagaacagag taccgcttta ccacattatg gatgttgcaa aacaaatgga
     2941 gaataattaa taacgctctc ttttagctcc attcttagcc tccttcttat gaccttaaca
     3001 aggcacccga cataaattcc tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat
     3061 cttggcttac gtggtatgcg tcagggcatt agcaataccc ttgattatca atagcataac
     3121 cataaccact gaaaattaaa aatttctata tatttcatat tgttaaatga tacttaaata
     3181 aatttcatca gtaaatcatt gccatagata atcattggat ggcacaatca attttcgcta
     3241 atgataacca ttcccattac gttttgtatg ttgatagcaa agtatctctg tctgctgaag
     3301 cccttttggc tgcgtaagaa taataaaact tcagtgttgt taatcattat tatcctcgcc
     3361 atgatccttg gcgtggttaa aattcaggtc tggttaaacg actggaataa tgattttttc
     3421 aatgcgctaa gccagaaaga aaccgataag ctttggcagc ttgtcctttg gttccctgcc
     3481 ctgctgggga tctttgtatt aatatccgta aacaaaacat ggttaattaa actactgact
     3541 attcgctggc gcgaatggtt aacagattac taccttaatc gctggtttgc agataaaaac
     3601 tactacttca cgcagatcta cggcgagcat aaaaatacag ataaccctga ccaacgtatc
     3661 gctgaagaca ttctcttact gatcagcaaa acgctcagtc tttcatttgg cttcatccag
     3721 tcacttagca tgctgatcac ctttaccgtt attctttggc aaagcgcggg tatgctctca
     3781 ttcactgttg gtggaacaga atggaatatc caggggtata tggtctatac cgttgtgctc
     3841 attgtgatcg gtggaacttt atttacccat aaggtgggta aacgtattcg tccgcttaat
     3901 gtggaaaaac aacgtagcga agcgacattt cgaactaatc ttgtgcagca taataagcag
     3961 gcagaactga ttgcgctaag caatgctgag tctctacaac gccaggagtt gagcgacaat
     4021 tttcatacca ttaaagagaa ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac
     4081 tggcagaata tttattcgcg ctcgcttagc gttcttccct actttctgtt attgccgcag
     4141 tttattagcg ggcagatcaa tctgggcgga ctgatgaaat cgcgccaggc atttatgctg
     4201 gtatcgaaca atctaagctg gtttatttat aaatatgatg aacttgctga actggctgcg
     4261 gttatcgatc gcttgtatga gttccatcaa ctcaccgaac agcgccctac gaataagcct
     4321 aaaaattgcc aacatgcggt acaagtggct gacgcgagta ttcgtactcc tgataataag
     4381 atcatattag agaacctgaa ctttcatgtt tcgccaggca aatggctatt actgaaaggt
     4441 tactctggtg cgggaaaaac cacactgctt aaaacattat cccactgctg gccgtggttt
     4501 aaaggtgata tttcttctcc tgccgacagt tggtatgtgt cacaaacacc gttaatcaaa
     4561 accggcttac tgaaagagat tatttgtaaa gcacttcccc tgcccgtaga cgataaatca
     4621 ttgagcgaag tactgcatca ggttggtctt gggaaattgg ctgcgcgtat tcatgaccac
     4681 gatcgctggg gagatattct ttccagcggc gaaaaacaac gtatcgccct ggcacgatta
     4741 attttacgac gtccgaaatg gatatttctt gacgaaacta cctctcatct tgaggaacaa
     4801 gaggctatcc gcttactgcg tctagtgcgt gaaaaactac ccacaagcgg cgtcattatg
     4861 gttacacatc aacccggtgt ctggaacctg gccgatgata tttgtgacat tagcgcggtt
     4921 ttataaaaac aataaaagcc ctgctgtacg gagatataaa taaatgaagc gagttcttat
     4981 tcctggcgtc attttatgtg gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta
     5041 catgcatttt tttgaagaga tgacggtcta tgctcctgtc cctgtacccg taaacggcaa
     5101 cacgcattac accagtgaaa gcatcgagcg tttaccgacc gggaatggca atatcagcga
     5161 tctgctgaga accaaccctg cggtacgcat ggattcaacg caaagtacct cgttgaacca
     5221 gggagatatt cgcccggaga aaatctctat tcacggtgcg tcgccctatc agaatgccta
     5281 tttgattgac ggtattagtg ccactaataa cctgaaccca gcgaatgagt ccgatgccag
     5341 tagtgcaacc aatattagcg ggatgtcaca ggggtattat cttgatgtca gcttactgga
     5401 caatgtgacg ctttatgaca gttttgtgcc ggttgaattc ggtcgcttca atggcggggt
     5461 aattgatgca aagttcaaac gcttcaacgc tgatgatagc agcgtgaaac tgggttatcg
     5521 cactacgcgt tcggactggt taacatcgca tatcaatgag aataacaaga gcgcatttaa
     5581 tcaaggctct tcaggaagta cttattactc cccagatttt aaaaagaact tttatacctt
     5641 gtcgtttaat caggaactcg ctgataactt tggcgttacc gccggtttat cgcgccgcca
     5701 gtctgatatc acccgcgcgg attatgtttc gaatgacggc attgtcgccg gtcgggcaca
     5761 gtataaaaac gttatcgata ctgcattgag caaatttacc tggtttgcca gcgaccgctt
     5821 tacccacgat ttaaccttaa aatataccgg ctccagccgt gattataata ccagcacctt
     5881 cccgcagtct gatcgcgaaa tgggtaataa atcctatggt atggcatggg atatggatac
     5941 gcagctcgca tgggccaaac tacgtaccac cgttggttgg gatcatatta gtgattatac
     6001 ccgtcacgat catgacatct ggtacaccga actttcatgt acatatggtg atattacagg
     6061 gcgttgcacc cgtggcggat taggacacat ttcccaggct gtagataatt acaccttcaa
     6121 aacacgcctg gactggcaaa aattcgccgt gggtaatgtt tcgcatcaac cctacttcgg
     6181 cgcggaatac atctattccg atgcgtggac tgaacgccat aaccagtctg aatcctatgt
     6241 gattaatgct gccggaaaga aaactaacca taccatttac cataaaggta aaggcagcct
     6301 gggaattgac aactacacac tgtatatggc ggatcgcatt agctggcgta atgtgtcatt
     6361 aatgcccggc gtgcggtatg actatgacaa ctatctgtca aaccacaata tctccccgcg
     6421 ctttatgacg gaatgggata tttttgctga tcaaacctca atgattacag caggttataa
     6481 ccgttactat ggcgggaata ttcttgatat gggattacgt gatatccgca atagctggac
     6541 ggaatcggta tcaggtaata aaaccctgac gcgttatcag gatttgaaaa cgccttataa
     6601 cgatgaactg gcaatgggat tgcagcaaaa aatcggtaag aacgttattg cgcgcgcaaa
     6661 ctatgtttac cgtgaagcgc atgatcaaat cagcaaaagc agtcgtaccg acagcgcgac
     6721 taaaaccacc attactgaat ataacaacga cggcaaaacc aaaacgcatt cgttcaacct
     6781 cagttttgaa ctggccgaac ccctgcatat cagccaggta gatattaacc cgcaaattgt
     6841 ctttagctat atcaagagca agggcaactt gtcgttaaac aatggttatg aggagagcaa
     6901 taccggtgat aaccaggtgg tttataacgg taatcttgtc tcttacgata gcgttccagt
     6961 ggcagatttt aataacccat taaagatctc cttaaacatg gatttcacgc atcaaccgag
     7021 cgggttggtg tgggcgaata cgctggcctg gcaagaagcg cgtaaagctc gcattatcct
     7081 gggtaagaca aatgcgcaat acatcagcga atattcagat tacaagcagt atgttgacga
     7141 aaaactggat agcagcctga cctgggacac ccgcttgtcc tggacgccac aatttctgaa
     7201 acaacaaaac ctgacgatca gtgccgatat tctcaatgta ctggatagca aaaccgcggt
     7261 tgatacaacg aataccggtg tggcgaccta cgccagtggc cgtactttct ggcttgatgt
     7321 cagcatgaaa ttttaatagt tcggatttta cttaaccggg taaattcgcc cggttttcgc
     7381 atggagataa ttatgagaaa cctctgtttc ttactgacgt tagtggcaac tctgttgctc
     7441 cccgggcggc tgattgccgc cgccttaccg caggatgaaa agttaattac cgggcaactg
     7501 gacaatggct tgcgatatat gatttatccg catgctcatc caaaggatca ggtaaattta
     7561 tggctgcaaa ttcataccgg ttcattgcag gaagaagaca atgagcgcgg cgtggctcat
     7621 tttgtagaac atatgatgtt taacggcaca aaaacatggc cgggtaataa agtcatcgaa
     7681 acatttgagt caatgggcct gcgttttggt cgcgatgtta atgcctatac cagctatgac
     7741 gaaacggtgt atcaggtgag tttgccaact acgcagaaac aaaatctgca acaagtgatg
     7801 gcaatcttca gtgaatggag taatgccgca acctttgaaa aactcgaagt agacgctgaa
     7861 cgcggcgtaa ttactgagga atggcgtgcc catcaggatg cgaaatggcg cacctctcag
     7921 gcgcgccgcc ctttcctgct ggcaaatacc cgtaatttag accgtgaacc tatcggcctg
     7981 atggatactg tcgccacggt cacaccggca caattgctcc aattttatca acgctggtat
     8041 caaccaaata atatgacctt tatcgtggtt ggcgatatcg acagtaaaga agcgctggcg
     8101 ctgataaagg ataatttaag taagcttccg gctaacaaag cagctgaaaa tcgcgtctgg
     8161 ccgacaaaag ccgaaaacca cctgcgcttt aatatcatta atgataaaga aaaccgggtg
     8221 aacggcatcg cactctatta tcgcctgcca atggtacaag tgaacgatga gcaaagcttt
     8281 atcgaacaag ctgaatggag catgttagtt cagctgttca atcaacgtct gcaggaacgt
     8341 atacagtcgg gcgagttgaa gactatttct ggcggcactg cgcgcagcgt taaaattgca
     8401 cccgattatc agtcgctgtt tttccgtgta aatgcacgag acgataatat gcaggatgct
     8461 gcgaatgcat taatggaaga gttggcaacc attgatcagc atggtttttc tgctgaagaa
     8521 ctcgatgatg tcaaatctac ccgcctcacc tggctgaaaa atgcggttga tcagcaagct
     8581 gaacgtgatt tacgtatgct gaccagtcgc ctggcatcca gctcattaaa taatacgccg
     8641 ttcttgtcgc cggaagagac atatcaactt tcgaaacgtc tgtggcagca aattaccgtg
     8701 caaagtctgg cggaaaaatg gcagcagtta agaaagaacc aggacgcatt ttgggagcaa
     8761 atggtaaaca atgaggttgc cgccaaaaaa gcattgtctc ctgcggctat cctggcgctg
     8821 gaaaaggagt acgccaacaa aaagctggcg gcttacatct tcccaggcag aaatttatcg
     8881 ttaacagtag acgctgaccc acaggcggaa attagcagca aagaaacgct ggctgagaat
     8941 ctgacatcat taacactttc caatggtgca agggttattc tggcaaaatc cgcgggtgaa
     9001 gagcaaaagc tacaaattat tgccgtatcg aataaaggcg atttaagttt ccctgcgcag
     9061 caaaaatcac ttatcgcgct ggcaaataaa gcagttagcg gaagcggcgt tggcgaactc
     9121 tcctcttcca gcctgaaacg ctggagtgcg gaaaactcgg taaccatgag cagtaaagtc
     9181 agtggcatga atacgttgct ctccgttagc gcgcggacta ataaccctga acctggtttt
     9241 cagttgatta accagcgaat cacccacagc acgattaacg acaatatttg ggcatcgcta
     9301 caaaatgctc aaattcaggc attgaaaacg ctcgaccagc gtccagcgga gaaattcgcc
     9361 cagcagatgt atgagacgcg ctatgctgat gaccgcacga aattactgca agaaaatcag
     9421 attgcacagt ttactgccgc agatgcgctg gctgccgatc gccagttgtt ttcatctcca
     9481 gcggatatca cgtttgtcat tgtcggtaat gtcgcagaag acaaactcgt ggcgttaatt
     9541 acgcgttact taggatcaat caaacactct gattcgccat tagccgcagg taaaccatta
     9601 actcgcgcga cggacaacgc atcggttact gtaaaggaac aaaatgaacc tgtggcacag
     9661 gtttcacagt ggaagcgtta tgattcccgg acacctgtta atctggcgac gcgtatggcg
     9721 ctcgatgctt ttaacgtcgc actggcaaaa gatctacgcg ttaatattcg tgaacaggca
     9781 tctggggcat acagcgtttc ttctcgcctc tcggttgatc ctcaggctaa agatatcagt
     9841 catttgctgg cttttacttg tcaaccagaa cgacatgatg aactgttaac gttagcgaat
     9901 gaagtgatgg ttaagcgttt gactaaaggg atcagtgagc aagaactgaa cgaataccag
     9961 caaaacgttc agcgcagcct cgatatccaa cagcgtagcg ttcaacaatt agcgaacact
    10021 attgtaaata gtcttattca atatgacgat cctgcagcat ggactgagca ggagcaattg
    10081 ttgaaacaaa tgacggtaga gaatgttaac actgccgtta aacaatatct ttctcatccg
    10141 gtaaatactt ataccggagt attattgcca aaataataac agccccgtca acacatcgtt
    10201 ggcggggatt ttagcaatat tcgctatttt tatgtaataa ttttataaat gcgttcaaaa
    10261 taataaccgc gtactaatag tgatatttta aggtctgatt tttacgtgat aattcaggag
    10321 gcacagaata cgcataaaaa taacagcata aaacacctta ctaccaccca agagctccct
    10381 actatattgt ttttcaataa aaaatattat tcacgcaata tctcaccata aataacatta
    10441 ggattttgtt atttaaacac gagtcctttg cacttgctta ctttatcgat aaatcctact
    10501 tttttaatgc gatccaatca ttttaaggag tttaaaatgg ataagaagca agtaacggat
    10561 ttaaggtcgg aactactcga ttcacgtttt ggtgcgaagt ctatttccac tatcgcagaa
    10621 tcaaaacgtt ttccgctgca cgaaatgcgc gacgatgtcg catttcagat tatcaatgac
    10681 gaattatatc ttgatggcaa cgctcgtcag aacctggcca ctttctgcca gacctgggac
    10741 gacgaaaacg tccacaagtt gatggattta tccattaaca aaaactggat cgacaaagaa
    10801 gaatatccgc aatctgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
    10861 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
    10921 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
    10981 ggcaaaccaa cggataaacc aaacctggtg tgcggcccgg tgcaaatctg ctggcataaa
    11041 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
    11101 atggatccga aacgcatgat tgaagcctgc gacgaaaata ccatcggcgt ggtgccgact
    11161 ttcggcgtga cctacaccgg taactatgag ttcccgcaac cgctgcacga tgcgctggat
    11221 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagtggtggc
    11281 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
    11341 tcgatcagtg cttcaggcca caaattcggt ctggctccgc tgggctgcgg ctgggttatc
    11401 tggcgtgatg aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctcggtggt
    11461 cagattggta ctttcgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac
    11521 tatgaattcc tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag
    11581 gttgccgctt atctggcgga tgaaatcgcc aaactggggc cgtatgagtt catctgtacc
    11641 ggtcgcccgg acgaaggcat tccggcggtt tgcttcaaac tgaaagatgg tgaagatccg
    11701 ggatacaccc tgtatgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc
    11761 ttcactctcg gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc
    11821 ttcgaaatgg actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc
    11881 agtgatcacc cgaaactgca gggtattgcc caacagaaca gctttaaaca tacctgataa
    11941 cgtttaacgg taacggtgtc ccgaaacgaa cccgtttcgg gacaatttcc aaagtctgtt
    12001 cactggcatt agcaacggaa aatattgttc tgaatacgct tcagaacaaa acaggtgcgg
    12061 ttccgacagg aataccgttt tagggggata atatggctac attagtacag acaggtaaag
    12121 ctaagcagct cacattactc ggcttttttg ccataacggc atcgatggta atggctgttt
    12181 atgaataccc taccttcgca acatcgggct tttcattagt cttcttcctg ctattaggcg
    12241 ggattttatg gtttattccc gtgggacttt gtgctgcgga aatggccacc gtcgacggct
    12301 gggaagaagg tggtgtcttc gcctgggtat caaatactct ggggccgaga tggggatttg
    12361 cagcgatctc atttggctat ctgcaaatcg ccattggttt tattccgatg ctctatttcg
    12421 tgttaggggc actctcctac atcctgaaat ggccagcgct gaatgaagac cccattacca
    12481 aaactattgc agcactcatc attctttggg cgctggcatt aacgcagttt ggtggcacga
    12541 aatacacggc gcgaattgct aaagttggct tcttcgccgg tatcctgtta cctgcattta
    12601 ttttgattgc attagcggct atttatctgc actccggtgc ccccgttgct atcgaaatgg
    12661 attcgaagac cttcttccct gacttctcta aagtgggcac cctggttgta tttgttgcct
    12721 tcattttgag ttatatgggc gtagaagctt ccgcaaccca cgtcaatgaa atgagtaacc
    12781 cagggcgcga ctatccactg gctatgttac tgctgatggt ggcggcaatc tgcttaagct
    12841 ctgttggcgg tttgtctatt gcgatggtca ttccgggtaa tgaaatcaac ctctccgcag
    12901 gggtaatgca aacctttacc gttctgatgt cccatgtggc accggaaatt gagtggacgg
    12961 ttcgcgtgat ctccgcactg ctgttgctgg gtgttctggc ggaaatcgcc tcctggattg
    13021 ttggtccttc tcgcgggatg tatgtcacag cgcagaaaaa cctgctgcca gcggcattcg
    13081 ctaaaatgaa caaaaatggc gtaccggtaa cgctggtcat ttcgcagctg gtgattactt
    13141 ctatcgcgtt gatcatcctc accaataccg gtggcggtaa caacatgtcc ttcctgatcg
    13201 cactggcgct gacggtggtg atttatctgt gtgcttattt catgctgttt attggctaca
    13261 ttgtgttggt tcttaaacat cctgacttaa aacgcacatt taatatccct ggtggtaaag
    13321 gggtgaaact ggtcgtggca attgtcggtc tgctgacttc aattatggcg tttattgttt
    13381 ccttcctgcc gccggataac atccagggtg actctaccga tatgtatgtt gaattactgg
    13441 tcgttagttt cctggtggta cttgccctgc cctttattct ctatgctgtt catgatcgta
    13501 aaggcaaagc gaataccggc gtcactctgg agccaatcaa cagtcagaac gcaccaaaag
    13561 gtcacttctt cctgcacccg cgtgcacgtt caccacacta tattgtgatg aatgacaaga
    13621 aacactaact atgtctgaat ggttataaga taagggagcg ataattcatc gctccctttt
    13681 tcgtgcttgc tgtcgtattg acttaaccgg gataagtacg agaatgagcg cacatctgtt
    13741 taccggaaac tagcacatat ggatatctgc tcccgaaaca agaaattaac gattagaaga
    13801 ccagcgatac tggttgcact ggcactttta ctgtgtagtt gtaaaagcac gcctccagag
    13861 tccatggtga caccaccagc gggttcaaag ccaccagcca cgacgcaaca atcgtcacaa
    13921 ccgatgcgtg gcatctggct ggccacggtg tctcgcctcg actggccacc ggtttcctcg
    13981 gttaacatta gtaaccccac cagccgggcc cgtgtacaac aacaggcgat gatcgacaaa
    14041 ctggatcatt tgcaacgtct cggtataaac acggtctttt tccaggtcaa gccggacggt
    14101 accgccctgt ggccatcgaa aattttgccg tggtccgatc ttatgaccgg taagattggt
    14161 gaaaatccgg gttacgatcc gctgcaattt atgctcgacg aagcccacaa gcgtgggatg
    14221 aaagtacacg cctggtttaa cccctatcgc gtatcggtta atacgaagcc cggtactatc
    14281 agggaactga atagcactct gtctcaacaa ccggcgagcg tctatgtgca acaccgtgac
    14341 tggatcagaa cgtctggcga tcgctttgtc ctcgacccgg gcatccctga ggttcaggac
    14401 tggatcacat caatagtcgc agaagtggtt tcccgctatc cggtagatgg cgtgcagttt
    14461 gacgactatt tctatactga gtcaccgggt tcacggctaa atgataacga aacgtaccgt
    14521 aaatacggag gcgcatttgc gtcaaaagca gactggcggc gcaacaatac tcagcagtta
    14581 attgcaaagg tatcgcacac cattaaaagc attaagccgg gagtcgaatt tggtgttagc
    14641 ccggcaggcg tgtggcgtaa ccgatcacac gatccgctcg gttccgatac ccgaggcgcg
    14701 gcagcctatg acgaatccta cgctgacact cgtcgatggg tggaacaagg attgctggat
    14761 tacattgctc cccaaattta ctggccgttc tcacggagtg ccgcgcgtta tgacgtgttg
    14821 gcaaaatggt gggcggatgt cgttaaaccg accaggaccc gcctgtatat cggtatcgcc
    14881 ttctataaag tgggtgaacc ttcaaagata gagccagact ggatgattaa cggcggcgta
    14941 ccggagctga aaaagcagct cgatcttaac gatgcggtac cagaaattag cggcaccatc
    15001 ttgttccgtg aggactatct gaataaaccg cagactcaac aagcggtcag ctatctgcaa
    15061 agtcgttggg gcagttaaac tcccattctt actttgtaac aagccggtgc tttacccact
    15121 ggcttgttac cctcttgaac tatcccacca gcaatcatgc ttactaaagt caaattaacc
    15181 acgcaattaa ccacgcttag ttaaacagcc ataaatcccc taataaaaat gtatgaatat
    15241 gaaaaaccat acgatattcg cagtcaccga aaaataatta cgatgggcta tactcttctg
    15301 aagaacagta gcgtagattg aaactgtcct aagtcgctta tttcttttca gtatatcttc
    15361 atatttcagg agaatatggt gcagttagtc gatctcgcac gtcgcgtttc attttgattc
    15421 cgtctgattt cccagcctta taagggtgtg aattatggag atgtatttta aaagaatgaa
    15481 agatgagtgg accggacttg tcgagcaggc agatccgccc attcgtgcta aagccgcgga
    15541 aattgccgtt gcgcatgctc attatctgag tatcgagttt tatcgaattg tccgcatcga
    15601 cccgcatgcc gaagaattct tgagtaatga acaagttgag cggcagttga aaagtgcgat
    15661 ggaacgctgg attattaacg tgctttctgc ccaggttgac gatgtcgaaa ggctaataca
    15721 aatccagcat accgtcgcgg aagtgcatgc ccgcatagga attccggtag aaattgtcga
    15781 gatggggttt cgggtgctga aaaagatcct ctatccggta atcttctctt cggattattc
    15841 tgccgcagaa aaacttcagg tctaccattt ctcgattaac agtattgata tcgctatgga
    15901 agtgatgacc cgcgcgttta cctttagtga cagtagtgcc tcaaaagaag atgaaaacta
    15961 tcgtatcttc tcgttactgg aaaacgccga agaagaaaaa gaacggcaaa tagcctcaat
    16021 actttcatgg gaaatagata ttatctataa aatcctgctg gattctgatt taggcagtag
    16081 tttgccttta agccaggctg attttggcct gtggtttaac cataaaggtc gacattattt
    16141 cagcggcatt gctgaagtgg gccatatctc tcgtctgatt caggatttcg acggtatttt
    16201 caatcaaacc atgcgtaaca ccaggaattt gaataacaga agtctgcggg tgaaattttt
    16261 attacagata agaaataccg tatcgcaaat tattaccttg ctgcgtgaat tgtttgaaga
    16321 agtatcgcgc cacgaagtcg gtatggatgt actgacgaaa ttacttaacc gccgtttcct
    16381 gccgactatc ttcaaacgcg aaattgccca tgccaaccgg accggtacac cgctgtcagt
    16441 gctgattatt gacgttgata aattcaaaga gatcaacgat acgtggggcc ataacactgg
    16501 cgatgaaatt ctgcgtaaag tctctcaggc cttttatgac aacgtccgca gtagtgatta
    16561 tgttttccgc tacgggggcg atgaatttat cattgttttg actgaagctt ctgaaaacga
    16621 aacgttacgt accgcagaac gtattcgcag tcgggtggag aaaaccaaac tgaaagccgc
    16681 aaacggcgaa gatattgccc tctcactttc catcggtgcc gccatgttta atggtcatcc
    16741 tgactatgag cgcctcattc aaatagccga tgaagctctg tatatcgcca aaagacgagg
    16801 tagaaaccgt gttgaactct ggaaagccag tctttagatg cgccaggatg cagaggtaat
    16861 catgaagcta accgatgcgg ataatgccgc cgatggcatt tttttccccg cccttgagca
    16921 aaatatgatg ggtgcggtgt taattaacga aaatgatgaa gtgatgtttt tcaaccccgc
    16981 cgcagagaag ctctgggggt acaaacgtga agaagtcatt ggcaataaca ttgatatgct
    17041 gattccgcgg gatttgcgtc ctgcgcatcc tgaatacatt cgtcacaacc gtgaaggcgg
    17101 taaagcgcgc gtcgagggga tgagtcggga gctgcagctg gagaaaaaag acggcagtaa
    17161 aatctggacc cgttttgcgc tatcgaaagt gagcgccgag gggaaagttt attacctggc
    17221 gctggtacgg gatgccagcg tagaaatggc gcaaaaagaa cagaccaggc aattgattat
    17281 tgccgttgac catctcgacc gaccggtgat tgtcctcgat ccggaacgcc atattgtgca
    17341 gtgcaatcgc gcatttaccg aaatgtttgg ttactgcatt agcgaagcca gcggtatgca
    17401 gcctgataca ctcctgaaca ttcctgaatt ccctgccgat aaccgcattc gtttacaaca
    17461 gttgctttgg aaaaccgccc gcgatcagga cgaatttctg ctgttgacgc gcaccggtga
    17521 aaaaatctgg attaaagctt ctatcagccc ggtttatgac gtgctcgcgc atctgcagaa
    17581 cctggtaatg accttctcgg atatcaccga agaacggcaa atccgccagc ttgaaggcaa
    17641 tattctcgcc gccatgtgca gcagcccgcc atttcatgaa atgggggaaa tcatttgtcg
    17701 taacatcgaa tctgttctca acgaatcgca tgtttcgctg ttcgcactgc gcaacgggat
    17761 gccgatacac tgggcgtcat cttcgcacgg tgcagaagtt caaaatgcgc aaagctggtc
    17821 agcgaccatt cgtcagcgtg atggcgcgcc tgcggggatc ctgcaaatta aaacctcgtc
    17881 aggagcagaa accagcgcct ttatcgaacg cgtggcagat atcagccagc atatggccgc
    17941 gctggcgctg gaacaggaaa aaagccgtca gcatattgaa caactcatcc aatttgatcc
    18001 gatgaccggt ctgccaaatc gcaataacct gcacaattac ctcgatgacc tggtcgacaa
    18061 agccgtctct ccagtatcgg tgttgaccat attcaggatg tgattgatag ccttggctat
    18121 gcgtgggccg atcaggcatt gctggaagtg gtcaatcgct ttcgtgaaaa actcaaaccg
    18181 gatcagtatc tctgtcgtat cgaaggtacg cagtttgtcc tcgtgagcct ggaaaacgac
    18241 gtcagtaaca ttacccagat cgccgatgag ctacggaatg tggtcagcaa gccgataatg
    18301 attgacgata accccttccc gcttaccttg agtattggca tcagctacga cgtgggtaaa
    18361 aatcgcgatt acctgctctc cactgcccat aacgcgatgg attatattcg caagaatggc
    18421 ggtaacggct ggcagttctt cagcccggcg atgaacgaaa tggtaaaaga gcgtttggtt
    18481 ttaggcgcag cgctgaaaga agcgattagc aataaccaac tgaaactggt ttaccagccg
    18541 caaatcttcg cagaaacggg tgaactctac ggcatcgaag cccttgctcg ctggcacgat
    18601 ccccagcatg gtcatgtgcc cccttcacgg tttattcctc tcgcagaaga gattggtgaa
    18661 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
    18721 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
    18781 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
    18841 ctgacggtag aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
    18901 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
    18961 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagtttt
    19021 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
    19081 gggcaaagcc tcaatttaac cgtcgtggcg gaaggcgtcg aaaccaaaga gcaatttgag
    19141 atgctacgaa agatccactg tcgcgttatt cagggatatt tcttttcccg ccccctaccc
    19201 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
    19261 ctctcccgca ctcgcgggtt ttcatttaac gtgacactgt cacttaaata ctgtgatttc
    19321 agctacgatt ccgggagatt ccttccttaa cttcccctct tgcaccaaaa ctgcactacg
    19381 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
    19441 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
    19501 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
    19561 agatcgaatt gaaatacgcc tgcgctgata acatcacagg taaagctatt taccagcaag
    19621 cgcgttgtct gttacacaag gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
    19681 agctatcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
    19741 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
    19801 acagccgtgg cacggcgatc gacctgacgc ttcgtgatga gcacgggaac atcctggata
    19861 tgggcgcagg gttcgatgaa atgcacgagc gctcccatgc ctatcaccct tccgtcccgc
    19921 ccgccgctca gcgcaatcgc ctgttgctga atgcgataat gactggtggc ggttttgtcg
    19981 gtatctccag cgaatggtgg cacttcgaat taccccaggc agcgagttac cctctgctcg
    20041 ctgatcaatt cacctgtttt atatcccctg gcacacagca cgtcagttaa tcaggaaact
    20101 gccatgaaga gatcgatatc gtttcgtccc acattgctcg cgctcgtcct tgccacaact
    20161 ttcccggttg cgcacgccgc cgtaccgaaa gatatgctgg tgattggtaa ggccgccgat
    20221 ccacaaaccc tcgacccggc ggtaacaata gataataacg actggacagt gacctacccg
    20281 tcttatcagc gactggttca gtacaaaacg gacggtgata aaggctcaac cgacgttgaa
    20341 ggcgatctgg caagtagctg gaaagcgtct aacgatcaaa aagagtggac gttcaccctg
    20401 aaagataatg ctaaatttgc cgatggcaca cctgtcactg ccgaagcagt aaaactctct
    20461 tttgagcggt tactaaaaat cggccagggg ccagcagaag catttcccaa agatttaaag
    20521 attgatgctc ccgacgaaca tacggtgaag tttaccctta gccagccatt tgcaccgttc
    20581 ctctacacgc tggcgaatga cggtgcatcc attatcaatc cggcggtatt aaaggaacat
    20641 gcggcggatg atgcccgcgg cttcctcgcg caaaataccg ccggttccgg accatttatg
    20701 ctgaaaagct ggcaaaaagg tcagcaatta gttctggtgc caaatccgca ttaccccggc
    20761 aataaaccga actttaagcg agtatcggta aaaattatcg gtgaaagtgc ctcccgtcgc
    20821 ctgcagctct cccgtggcga cattgacatt gccgatgcgc tgccggtgga tcaactcaac
    20881 gccctgaagc aggaaaacaa agtcaatgtg gcagagtatc cgtcactgcg cgtcacctat
    20941 ctgtatctga ataacagcaa agcgccactt aatcaggcgg atctgcggcg ggccatttcc
    21001 tggtctaccg attatcaggg catggttaac ggcattctga gtggtaacgg aaaacaaatg
    21061 cgcggcccga ttccggaagg catgtggggc tacgatgcga cggcaatgca atacaaccat
    21121 gacgaaacga aagccaaagc cgaatgggat aaagtgacga gcaaacccac cagcctgacg
    21181 tttctctact ccgataacga tccgaactgg gaaccgatcg ctctggcaac acaatccagc
    21241 ctcaacaagc tgggcatcaa tgtgaagctg gaaaagctgg cgaacgccac catgcgcgac
    21301 agagtgggta aaggtgatta cgacattgcg attggcaact ggagtccgga ttttgccgac
    21361 ccgtatatgt ttatgaatta ctggtttgag tcagacaaaa aaggtctgcc gggtaaccgc
    21421 tcgttctatg aaaacagtga ggtcgataag ttactgcgca atgcgcttgc gaccaccgac
    21481 cagacgcagc gtacccgaga ctaccagcag gcacagaaaa tcgtcattga tgacgctgct
    21541 tatgtgtacc tgttccagaa aaactaccaa ctggcgatga acaaagaggt gaaaggcttt
    21601 gtgttcaatc ccatgctgga acaggtcttc aatatcaata ccatgagtaa ataacgtgac
    21661 cttctggagt attttacgcc aacgctgctg ggggctggtg ctcgtggtgg cgggcgtctg
    21721 cgtgattacg tttattatct cgcatctgat ccccggcgat ccggcgcggt tactggcggg
    21781 tgaccgcgcc agcgatgcta tcgtggaaaa tattcgccag caactgggac tggaccagcc
    21841 actgtatgta cagttttacc gctacgtcag cgacctgttt catggtgacc tgggaacatc
    21901 cattcgtact gggcgtccgg tgctggaaga gttgcgtata tttttcccgg cgacgctgga
    21961 gctggctttt tgtgccctgc tgctggcact cttgattggc atcccgctgg gcatactctc
    22021 tgcagtctgg cgaaatcgct ggctggatca tctggtgcga ataatggcga ttaccggaat
    22081 ctccacacct gcgttctggc ttgggctggg cgtcatcgtg ctgttttatg gtcatctgca
    22141 aatacttccc ggcggcggaa ggcttgatga ctggctggat ccaccaacgc acgttaccgg
    22201 cttttatctg ctcgatgcgt tgcttgaagg caacggtgaa gtcttcttca atgcgttgca
    22261 acatctcatc ttaccggcat taacgctggc gtttgttcac ctggggattg tcgcccgcca
    22321 gatccgctca gcgatgctgg aacaattgag tgaagactac attcgtaccg cccgggccag
    22381 cggcttgccc ggctggtata tcgttctatg ttatgcgcta cccaatgcgt tgatcccttc
    22441 gattaccgta ttggggctgg cgctgggcga tttgttgtat ggcgcagtgc tcaccgaaac
    22501 cgtttttgcc tggcccggaa tgggggcatg ggtagtgaca tcaatacagg cgctcgactt
    22561 cccggcagtg atgggctttg ccgtcgtggt ttcatttgct tatgtgctgg tcaacctggt
    22621 ggtggatttg ctctatttgt ggattgatcc gcgtatcgga cgtggaggtg gtgaatgatg
    22681 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
    22741 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
    22801 atgctactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
    22861 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
    22921 cgcgatctgt tcagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtc
    22981 gtggttgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
    23041 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
    23101 gtactgacaa tggcactggc tgccgctctc gggccgagtt tgtttaacgc catgctggct
    23161 attgctattg tgcgaattcc cttttatgtg cgcctggcgc gggggcaagc attagttgta
    23221 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
    23281 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
    23341 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
    23401 agtgctgaat ggggcgcgat ggtagcaaat ggtcgcaact atgtactcga tcagtggtgg
    23461 tattgcgcat ttccgggggc agcgattttg cttaccgccg tcgggtttaa tctctttggt
    23521 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
    23581 ttctggacat tcaacaactg catttgagtt tccccggttt taacggcgac gttcacgcgc
    23641 tcaacaatgt gtccttgcag attaaccgcg gtgaaattgt cggtctggtg ggagaatccg
    23701 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
    23761 gcgtacatcg ggggcagatt tcactgctgg gagacgatgt tcttaacgcg cgggaaaaac
    23821 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
    23881 tcaatccgac acgtcgaata ggtcttcaga tgatggacgt gatccgccat catcaaccaa
    23941 taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg caaatcccgg
    24001 ataccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
    24061 taatgattgc gctggcattc tcctgcgaac cgcaattgat tattgccgac gaaccgacta
    24121 cggcgctgga cgtcacggta cagttgcagg tactgcgttt gcttaaacat aaagctcgcg
    24181 acagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
    24241 atagcgttta cgtgatgtat gccggaagcg tgattgagag cggcgtgacg gcagacgtta
    24301 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
    24361 taccacgcca gccattaccc gccattccag ggacggtacc aaatctcacc catttgcctg
    24421 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
    24481 cgctgacagc gtgtggtgac aacaaccagc gctgcgcctg ttggtatccg cagcaggagg
    24541 tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt cccggcgcgt
    24601 aaaaactggc ttggtaaaac tacggaacat gttcatgcca ttaatggtat tgatttacag
    24661 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
    24721 gcacagcttt taatgggtat gctgcaaccg agccacgggc agtacatccg ttctggctca
    24781 caacgcatta tgcagatgat gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg
    24841 gtgtggcgca tcatcacaga accgctctgg atagctaagc atagtagtga acaacagcgg
    24901 cgagcgttgg cagaggagct ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
    24961 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
    25021 tcgcagcctg acgtgattgt gcttgatgag ccaacctctg cgctggatat ctccgtgcag
    25081 gcgcagatcc tcaatttact ggtaacgcta caggaaaatc acgggctgac ttatgtgctg
    25141 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
    25201 gggcagattg tggaattagg ggaagcgcaa caggtgctaa cggctccggc acatccatac
    25261 acccgattat tgctggattc cctccccgcc attgataaac cactggagga agaatgggca
    25321 ttacgtaaaa cggatctgcc aggaaaccgc acgttgccac agggctgttt tttccgcgaa
    25381 cgttgccccc ttgcaaccag cggatgcgaa gtccggcaat cattaacgac gcgggctgac
    25441 ggacgtgaga tccgttgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
    25501 tgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
    25561 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
    25621 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
    25681 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatc gatgttggcg
    25741 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
    25801 cgccaatcag ttcttcaggg ttggttcctt tttcgccttc aaaacgcgtg ttaaatccat
    25861 acggctgttg gttcagcacg ccactctcgg tggatactgt tcccttcccg cgtttgatat
    25921 cgctttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc ctgtgggctt
    25981 aaaaaggaaa ttatagacta cgtgagaaac cgcacgagga taagcaggat attccgaata
    26041 aaatacggca aaaatattac ttctgcactt aaattaaatt cccacaatag ataaccggaa
    26101 atatatatct caagagagat tgcatcaata ttaatcaact catcacaata ataataccta
    26161 tcaatatcat ggggttaaaa tataaaaatc atcaaccagg actaatctta acaacgaagc
    26221 ggcaaatatt tgccatgctg aatgtgcttt ataaatctgc gatccgtagc agacactata
    26281 aatacacaga cacggagaat cactatgttt acttattatc aggcagaaaa ttcaacagca
    26341 gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcacgg tgttgtcact
    26401 gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa cgacatcctc
    26461 agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc tacgctttaa
    26521 gatgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct tcgctacggc
    26581 gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg aaccgtcagg
    26641 cacgtcatat tcttggactg gaccataaaa tatctaacca gcgcaaaata gttaccgaag
    26701 gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct gaaaagtaat
    26761 tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg gtgtttttgt
    26821 ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac gataaaagcc
    26881 ccccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca aaaaaacagc
    26941 gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg ttgaataaag
    27001 gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta ctgccggaag
    27061 tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag ggattcaaaa
    27121 ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa accctcttct
    27181 accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc ccaaccgtcg
    27241 gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg tttatctctt
    27301 accagaaccg ccacaatatg gacgatattc tgcaaaacgt gccgaaccat aatattaaag
    27361 tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc atcggcggga
    27421 tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc agcccggcgt
    27481 atacccttcc ggtggtgctg gatgtcggaa cgaacaacca acagctgctt aacgatccgc
    27541 tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa ttcgttgatg
    27601 aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt gaagactttg
    27661 ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt tcctttaacg
    27721 atgacattca gggcaccgcg gcggtaacag tcggcaccct gatcgcagca agccgcgcgg
    27781 caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca gcgggatgcg
    27841 gcattgccga aatgatcatc gcccagaccc agcgcgaagg attaagcgag gaagcggcgc
    27901 ggcagaaagt ctttatggtc gatcgctttg gcctgctgac cgacaagatg ccgaacctgc
    27961 tgcctttcca gaccaaactg gtgcagaagc gcgaaaacct cagtgacagg gataccgaca
    28021 gcgatgtgct gtcactgctg gatgtggtgc gcaatgtaaa accagatatt ctgatcggcg
    28081 tctcaggaca gaccgggctg tttacggaag agatcatccg tgagatgcat aaacactgtc
    28141 cgcgtccgat cgtgatgccg ctgtccaacc cgacgtcacg cgtggaagcc acaccgcagg
    28201 acattatcgc ctggactgaa ggtaacgcgc tggtcgccac gggcagtccg tttaatccag
    28261 tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt attttcccgg
    28321 gcatcgggct gggtgttatt gcttccggcg cgtcacgtat caccgatgag atgctgatgt
    28381 cggcaagtga aacgcttgcc cagtattcgc cgctggtcct gaacggcgaa ggtctggtac
    28441 taccggaact aaaagatatt cagaaagtct cccgcgcaat tgcgtttgcg gttggcaaaa
    28501 tggcgcagca gcaaggcgtg gcggtgaaaa cgtctgccga agctttgcaa caagccattg
    28561 acgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc taagcctgcg
    28621 cccggtagtg aaggctaccg ggctatttcc ctctcccttt ttcagatctc atccatactg
    28681 ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat ccgaaaagga
    28741 ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac ggataaaaca
    28801 ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg tgtatgtcat
    28861 accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ccggcgtaat tctgggccat
    28921 gaagggattg gtgtggtggc agaagtgggt ccaggtgtca cctcattaaa accaggcgat
    28981 cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg taacagtggt
    29041 aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg cgggatggcg
    29101 gaagagtgca tcgtggtcgc cgattacgcg gtaaaagtgc cagatggtct ggactcggcg
    29161 gcggccagca gcattacctg tgcgggggtc accacctaca aagccgttaa gctgtcaaaa
    29221 attcgtcccg ggcagtggat tgctatctac ggtcttggcg gtctgggtaa cctcgccctg
    29281 caatacgcga agaatgtctt taacgcgaaa gtgatcgcca ttgatgtcaa tgatgagcag
    29341 ttaaaactgg caaccgaaat gggtgcagat ttagcgatta actcacgcac cgaagacgcc
    29401 gccaaaattg tgcaggagaa aaccggtggc gctcacgctg cggtggtaac agcagtagct
    29461 aaagctgcgt ttaactcggc agttgatgct gtccgtgcag gcggtcgtgt tgtggctgtc
    29521 ggtctgccgc cggagtctat gagcctggat atcccacgtc ttgtgctgga tggcattgag
    29581 gtggtcggtt cgctggtcgg cacgcgccag gatctaactg aagccttcca gtttgccgcc
    29641 gaaggtaaag tggtgccgaa agtcgccctg cgtccgttag cggacatcaa caccatcttt
    29701 accgagatgg aagaaggcaa aatccgtggc cgtatggtga ttgatttccg ccgctaagag
    29761 gcctttgctg cgactcccat gttcgggtcg cagcatcgca cactctccaa catgaaatgg
    29821 ctaaatggat tattgttgcg taggaaggaa caataatgat agtgttccca gagaaggaac
    29881 gctactgaga gtaaagacac taatgaaaat ggccaatcat ccccgcccgg gggacattat
    29941 tcaggaatca ctggacgaac ttaatgtcag cctgcgcgag tttgccagag caatggaaat
    30001 tgctccctca acggcaagcc gattgctgac cggaaaagca gctttgacgc cagaaatggc
    30061 aattaaactt tccgtggtga tcggcagttc gccgcaaatg tggctgaatc tgcaaaacgc
    30121 ctggagtctg gcagaggctg caaaaacggt ggatgtgtcg cgattacgcc gtctggttgc
    30181 gcaataaaaa atggcgcctt ttacagcgcc atttgaaact gatgacaaac gcaacattgc
    30241 ctgatgcgct acgcttatca ggcctacacg ttcactgcaa tctattggat ttgaacggtt
    30301 tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa caaagcctac gttgtctaca
    30361 atatgaaaat ggcgcctttt acagcgccat tttcagctta aagttcaaaa gagattatat
    30421 cccttcttca ctctcttttt tcgcttctgc cttctcgatt tcacgatacc agcgcggatg
    30481 gtgtttcttc gcccagcgac ggctcacctt cccttcgatc atccctttaa tcgatccttt
    30541 cacccagaat gccatataca tatggatcag gatggcgtgg atcaggatga tacccgcagc
    30601 cgcgtggatc agcaggctgt agcgaacaac ctgcatcggg aagtactgcg caaagtacgg
    30661 acgccagata atcaccccgg tcaccagcag cacgaaaatc atgctcatga tcgaccagaa
    30721 catcatcttt tgcccggcgt tgtacttacc gacatccgcc actttatgct cattgccttt
    30781 caatacttcg acaatgttta acagccacgg aatatctttc ttatccggga tgttgtgatg
    30841 cacaaaacgc acaaacataa acatcagtgc gacgaaaatc gcaatgccga agaatgggtg
    30901 caaaatgcgt cccatctgcg gcgtaccgaa ggtttgcgtc agccattgca gcgtcgggaa
    30961 gaagaacgaa atcccggaca gcgccaccag gaagaagcaa atcaccacgg tccagtgaca
    31021 ggcgcgatca ataaatttgg tgcgcacaat cattttcgac ttactcatga tgatcctcct
    31081 cgtcatcgtc cacttcctta ttcgggccaa taccgatgta gtggaaaatc aaccctgcaa
    31141 aagtggcaat aaagccagcc gctgccagcg gtttcaacgc gcctttccac aggcttaccg
    31201 aggtgtcgat cttcggatct ttcggcagac cgtgatacag ctccggctga tcggcgtgat
    31261 gcagcacgta cataacgtgc gtaccaccga ccccttccgg gttgtagacg ccagcatgtt
    31321 cgtaaccacg cgctttcagt ttcgccacgc gctgttccgc cagctccagc atctccttct
    31381 tggtgccgaa gtggatagcc ccggtcggac aggttttcac acaagccggt tcctggccga
    31441 cgctgacgcg atcgacgcag agcgtgcatt tatatacccg gttatcctct ttgttgaggc
    31501 gcggaatatt aaacggacac ccggcaatgc agtaaccaca gccgatgcag ttttccgact
    31561 ggaaatcgac aatcccgtta gcgtactgaa tgattgcacc ggcagacggg cacgccttca
    31621 ggcagcccgg atcttcacag tgcatacagc catctttacg gatcagccac tccagcttgc
    31681 cgctctgttc ggtttcgcta aagcgcatca ccgtccagga cttggcgctc agatcggcgg
    31741 ggttatcgta aaccccgacg cagtgcccca cttcatcacg gatgtcgttc cactccgaac
    31801 acgccacctg acaggcttta cagccgatac aggtggaaac gtcgataagt tttgcgactt
    31861 ctgctttgta atcacgcacc tgagaaggcg gcgtgatgga gttagttgcg gaccttttga
    31921 taatgtcctg cgtttccata gccatctgtt cgccccctta cgccttctcg atgttgacta
    31981 agaacgcttt atattccggc gtttgcgagt ttgcatcacc gacattcggc gtcagagtgt
    32041 tagctatata acctttacgc gcgacaccct caaagcccca gtggattgga atacccaccg
    32101 tttcaacctg ctgaccattt acattcagcg gtttcagacg acgcgttacc acagccaccg
    32161 cgcggataaa gccacgcttg cttgagacag tgacacgatc gccattatta atgcctttcg
    32221 ccgccgccag cgtttcgctg atttccacaa actgttccgg ctgagcaatt gcgttgagca
    32281 acgcgtgctt ggtccaggtg tggaagtgct cggtcagacg ataggtcgta cccacatacg
    32341 ggaactgctc ttttttaccc atccgcagcg cgtcttgttc atacagacga accaccgggt
    32401 tagaaaccac gttcggatgc agcgggttag ttcccagcgg cgtttcaatt ggctcgtagt
    32461 gttccgggaa cggaccttcc gccattttgt tgatggcaaa caggcgtccc atcccttccg
    32521 gctgcatgat aaacggcccg gttggcgtac ccggtgcggc attgccgaag tcagggatat
    32581 cgttacccgt ccacttgctg ccgttccact ggatcagcat ccgtttcgga tcccacggtt
    32641 taccgttgat atctgccgaa gcacggttgt agagtacgcg acggttaagc ggccacgccc
    32701 aggcccatcc cagcgtattc cccagaccgg acgggtcgga gttatcgcgg ttagccatct
    32761 ggttgccctg ctctgtccag ctaccggtgt agatccagca agaagatgcg gttgtaccgt
    32821 catcacgcag atgcgcaaag ctactcagca actgaccttt cttcgcaatc agcacgccat
    32881 tagcatcata gagatcttcc agcgcatagc cgttgttctc tttagctact tcgtcagatt
    32941 gcggttcgtg cggctgcttg tagttccagc tcatcttcat cagcggttct acgcctttac
    33001 caccttcagc ctggtacagc tcgcgcagat gatggtagat acccgccaga atttcgccgt
    33061 cgttacgcgc ttcgcccggc gcgtcctgac ctttccagtg ccactgcaac cagcgaccgg
    33121 agttagcgat agaaccatct tcttctgcaa agcaggtcga aggcagacgg aatacttcag
    33181 tctgaataga cgccggatcg acatcgttcg actcgccgtg gttctgccag aaggtagagg
    33241 tttcagtcac cagcggatcg ataaccacca tgtacttcag cttgctcagg cagctcacca
    33301 ctttgttttt gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa taaccggtga
    33361 ctttgccttc atccatcatg ttgaaatact tgatgacgtc gtaggtctgg tcccacttcg
    33421 gcagccagtc atagccccag ttgttctctt tctgcgcggc atcgccatag aaagatttca
    33481 tcaggctaac gaagaacttc ggatagttgc tccagtagtt cacctgacca gccagcgtcg
    33541 ctttcggcgt gttcgcttcc agatacgact gcaaatcaac ctgtttttct gacggcagcg
    33601 tcagataacc tggcaggctg gtagagagca gacctaagtc agtcagaccc tgaatgttgg
    33661 agtgaccacg caatgcattc acgccgccac cggccatacc catgttaccg agcagcaact
    33721 ggatcatcgc catagtacgg atgttctgcg cacccacggt gtgctgcgtc cagcccagcg
    33781 cgtacaggaa ggtggttgtg cgatccggtg cgctggtgga ggccagcact tcacacactt
    33841 tcaggaagtc ggcttttggc gtaccacaga tgttttcaac aacgtccggc gtgtagcggg
    33901 aaacgtgctc tttcagcagg ttccacacac agcgcggatg agtcagtgtt tcatcgcgtt
    33961 ttgcatagcc gttttcatcg aactgatagt tccaggacga cttatcgtac tggcgttttt
    34021 cagcgtcgta gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc accagcaggc
    34081 tggcgttggt gtaatgctta acgtattcgg cgttgatttt gttgttttcg atcaggtagc
    34141 gcaaaacgcc agacaggaac gtaatgtccg taccggaacg aataggcgcg tagatatccg
    34201 ccacagaagc ggtacgcgta aaacggggat cgacaacgat caaggttgca tcgttgttgt
    34261 ttttcgcttc catcgcccag cggaaaccga cgggatgcgc ttcagcagcg ttaccgccca
    34321 ttaccattac gacgttagcg tttttgatat ccacccagtg gttggtcatc gcaccgcgac
    34381 caaatgttgg agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc tggttgtcta
    34441 ccgccagcat cccgagggag cgggcaaatt tctgggtcag catcccggtt tcgttgctgg
    34501 caccggaagc acacagcata ccggtagaaa gccaacggtt taccgttacg ccctgctcgt
    34561 tcttttcaat aaagttagcg tcacggtcag ctttcatcag cttcgcaata cgggagaatg
    34621 cttcttccca gctaatgcgc tgccatttgt cagaacctgg cgcacgatat tccgggtagc
    34681 gcagacggtt ttcgctgttg acgtaatcca gcaaaccggc ccctttcggg cacagcgccc
    34741 cacggcttac cggatgatcc gggtcacctt caatgtgata aatcgcttct ctggcgtttt
    34801 tcgcgccatc acccaggcta tacatcaata gcccgcaacc tacggaacag tatgtgcagg
    34861 tgttacggat ctctttagcg cgtaataatt tgtagtttcg cgcctgagcc agtgcttgct
    34921 tcggggcaaa gcccaatgcc gctactgttg ttccagccat accgcccgcg cagattttaa
    34981 aaaattgtct gcgactgacg tccattgctt tcctcttttt tcaggggtat tactgcgcga
    35041 ggaaactaac agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac acgtgtttgc
    35101 cacaaaataa tccgcctgcg caaattgacc tacctcaata gcggtagaaa aacgcaccac
    35161 tgcctgacag gccagttaaa aaaatgctat aaaattcagc ttaattttta acggcaagag
    35221 agacaaaaca gcgagcatga cacgacaaaa agcaacgctc atagggctaa tagcgatcgt
    35281 cctgtggagc acgatggtag gattgattcg cggtgtcagt gaggggctcg gcccggtcgg
    35341 tggcgcagct gctatctatt cattaagcgg gctgctgtta atcttcacgg ttggatttcc
    35401 gcgtattcgg caaatcccga aaggctattt actcgccggg agtctgttat tcgtcagcta
    35461 tgaaatctgt ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg cgattgaagt
    35521 gggtatggtg aactatctgt ggcccagcct gacaattctc tttgccattc tgtttaatgg
    35581 tcagaaaacc aactggctga ttgtaccagg attattatta gccctcgtcg gcgtctgctg
    35641 ggtgttaggc ggtgacaatg ggttacatta tgatgaaatc atcaataata tcaccaccag
    35701 cccattgagt tatttcctgg cgttcattgg tgcgtttatc tgggcagcct actgcacagt
    35761 aacgaataaa tacgcacgcg gatttaacgg aattaccgtt tttgtcctgc taacgggagc
    35821 aagtctgtgg gtttactatt ttctcacgcc acaaccagaa atggtattta gcacgccagt
    35881 catgattaaa ctcatctctg cggcatttac cttaggattt gcttatgctg catggaatgt
    35941 cggtatactg catggcaacg tcaccataat ggcggtaggt tcgtatttta cgcccgtact
    36001 ttcctcagcg cttgcagccg tgctgctcag cgccccgctg tcgttctcgt tctggcaagg
    36061 cgcgctgatg gtctgcggcg gttccctgct ctgctggctg gcgacacgtc gtggctaaaa
    36121 taattgccgg gtctacggcc cggcaattaa tcagataaac cccgacaata ttaaaaaata
    36181 aatttcaaat accagtaatt caccttatat ctcatttcga ctccgatcac atatcgataa
    36241 ataatattat ttcatctatt tttctcattg tttttaatc
//
LOCUS       DAZFDC010000032        36140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFDC010000032 DAZFDC010000000
VERSION     DAZFDC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.032"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(175..2442)
                     /gene="adiA"
                     /locus_tag="ACHJ48_001739"
     CDS             complement(175..2442)
                     /gene="adiA"
                     /locus_tag="ACHJ48_001739"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO4707314.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFAADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2641..3549)
                     /gene="melR"
                     /locus_tag="ACHJ48_001740"
     CDS             complement(2641..3549)
                     /gene="melR"
                     /locus_tag="ACHJ48_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO4707315.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACIPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3832..5187
                     /gene="melA"
                     /locus_tag="ACHJ48_001741"
     CDS             3832..5187
                     /gene="melA"
                     /locus_tag="ACHJ48_001741"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO4707316.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYAPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5290..6711
                     /gene="melB"
                     /locus_tag="ACHJ48_001742"
     CDS             5290..6711
                     /gene="melB"
                     /locus_tag="ACHJ48_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO4707317.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKIPPEPVHADIPVGAVSDVKA"
     gene            complement(6850..7479)
                     /gene="yjdF"
                     /locus_tag="ACHJ48_001743"
     CDS             complement(6850..7479)
                     /gene="yjdF"
                     /locus_tag="ACHJ48_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO4707318.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7602..9248)
                     /gene="fumB"
                     /locus_tag="ACHJ48_001744"
     CDS             complement(7602..9248)
                     /gene="fumB"
                     /locus_tag="ACHJ48_001744"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4707319.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSVETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEKAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHHPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIINKQCANCTK"
     gene            complement(9326..10666)
                     /gene="dcuB"
                     /locus_tag="ACHJ48_001745"
     CDS             complement(9326..10666)
                     /gene="dcuB"
                     /locus_tag="ACHJ48_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO4707320.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11237..11956)
                     /gene="dcuR"
                     /locus_tag="ACHJ48_001746"
     CDS             complement(11237..11956)
                     /gene="dcuR"
                     /locus_tag="ACHJ48_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO4707321.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11953..13584)
                     /gene="dcuS"
                     /locus_tag="ACHJ48_001747"
     CDS             complement(11953..13584)
                     /gene="dcuS"
                     /locus_tag="ACHJ48_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO4707322.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13765..13995
                     /gene="yjdI"
                     /locus_tag="ACHJ48_001748"
     CDS             13765..13995
                     /gene="yjdI"
                     /locus_tag="ACHJ48_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO4707323.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            14007..14279
                     /gene="yjdJ"
                     /locus_tag="ACHJ48_001749"
     CDS             14007..14279
                     /gene="yjdJ"
                     /locus_tag="ACHJ48_001749"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4707324.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14506..14802
                     /gene="ghoS"
                     /locus_tag="ACHJ48_001750"
     CDS             14506..14802
                     /gene="ghoS"
                     /locus_tag="ACHJ48_001750"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4707325.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14830..15003
                     /gene="ghoT"
                     /locus_tag="ACHJ48_001751"
     CDS             14830..15003
                     /gene="ghoT"
                     /locus_tag="ACHJ48_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO4707326.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15122..16639)
                     /gene="lysS"
                     /locus_tag="ACHJ48_001752"
     CDS             complement(15122..16639)
                     /gene="lysS"
                     /locus_tag="ACHJ48_001752"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO4707327.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16876..18333)
                     /gene="dtpC"
                     /locus_tag="ACHJ48_001753"
     CDS             complement(16876..18333)
                     /gene="dtpC"
                     /locus_tag="ACHJ48_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO4707328.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESH
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            complement(18392..20539)
                     /gene="cadA"
                     /locus_tag="ACHJ48_001754"
     CDS             complement(18392..20539)
                     /gene="cadA"
                     /locus_tag="ACHJ48_001754"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO4707329.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20619..21953)
                     /gene="cadB"
                     /locus_tag="ACHJ48_001755"
     CDS             complement(20619..21953)
                     /gene="cadB"
                     /locus_tag="ACHJ48_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO4707330.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22319..23857)
                     /gene="cadC"
                     /locus_tag="ACHJ48_001756"
     CDS             complement(22319..23857)
                     /gene="cadC"
                     /locus_tag="ACHJ48_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO4707331.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24117..24276)
                     /locus_tag="ACHJ48_001757"
                     /pseudo
     CDS             complement(24117..24276)
                     /locus_tag="ACHJ48_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(24474..24549)
                     /locus_tag="ACHJ48_001758"
     tRNA            complement(24474..24549)
                     /locus_tag="ACHJ48_001758"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24514..24516),aa:Phe,seq:gaa)
     gene            complement(24656..25231)
                     /gene="yjdC"
                     /locus_tag="ACHJ48_001759"
     CDS             complement(24656..25231)
                     /gene="yjdC"
                     /locus_tag="ACHJ48_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4707332.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(25268..26965)
                     /gene="dsbD"
                     /locus_tag="ACHJ48_001760"
     CDS             complement(25268..26965)
                     /gene="dsbD"
                     /locus_tag="ACHJ48_001760"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO4707333.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPVDQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLLTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGTIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(26941..27279)
                     /gene="cutA"
                     /locus_tag="ACHJ48_001761"
     CDS             complement(26941..27279)
                     /gene="cutA"
                     /locus_tag="ACHJ48_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO4707334.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(27395..28696)
                     /gene="dcuA"
                     /locus_tag="ACHJ48_001762"
     CDS             complement(27395..28696)
                     /gene="dcuA"
                     /locus_tag="ACHJ48_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO4707335.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(28814..30250)
                     /gene="aspA"
                     /locus_tag="ACHJ48_001763"
     CDS             complement(28814..30250)
                     /gene="aspA"
                     /locus_tag="ACHJ48_001763"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO4707336.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            30587..31063
                     /gene="fxsA"
                     /locus_tag="ACHJ48_001764"
     CDS             30587..31063
                     /gene="fxsA"
                     /locus_tag="ACHJ48_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO4707337.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(31079..32335)
                     /gene="yjeH"
                     /locus_tag="ACHJ48_001765"
     CDS             complement(31079..32335)
                     /gene="yjeH"
                     /locus_tag="ACHJ48_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO4707338.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPILIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            32611..32904
                     /gene="groS"
                     /locus_tag="ACHJ48_001766"
     CDS             32611..32904
                     /gene="groS"
                     /locus_tag="ACHJ48_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO4707339.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            32948..34594
                     /gene="groL"
                     /locus_tag="ACHJ48_001767"
     CDS             32948..34594
                     /gene="groL"
                     /locus_tag="ACHJ48_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO4707340.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            34732..35085
                     /gene="yjeI"
                     /locus_tag="ACHJ48_001768"
     CDS             34732..35085
                     /gene="yjeI"
                     /locus_tag="ACHJ48_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO4707341.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(35278..>36140)
                     /gene="yjeJ"
                     /locus_tag="ACHJ48_001769"
     CDS             complement(35278..>36140)
                     /gene="yjeJ"
                     /locus_tag="ACHJ48_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO4707342.1"
                     /translation="SIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLL
                     IALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLEL
                     TDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDF
                     LPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREA
                     TPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQ
                     STSKTAPQAK"
BASE COUNT         9265 a         8661 c         9068 g         9146 t
ORIGIN      
        1 acatattttt ttacagggta aataacaggc aaaaaaaagc gcggggtggg gccgcgcaaa
       61 aagtatgaca tttttgtttg cagcaatttt tatgaaaacg ttgccatact caaaaggcct
      121 aaggccaggc ggcgtaatgt tatttaaaca attacgcctt cagcggaata gtggttacgc
      181 tttcacacac ataacgtggt aaataccgtc aataatttca gtcccttcag tttcgtgttc
      241 aaatccaggg aaatgatggt cccaggattg cagcgagcgt aaataactta cttgcggact
      301 gtttttatcg ccgaagtttt caccagacag cagcatcggg attcctggcg gatacgggat
      361 aactgagttt gccgcgatac gtcctggcag attttcaatg gataccagtt cgacattgtt
      421 gtcgacaatc gcgttgtacg cttcacgcgg ggtgatttcc gccaccggca ggccggaata
      481 ggcttcgttc aaccgtgcgc cagggttgtt ttctttcagc caggcaaaca tggtgtcacc
      541 cagatcgtga atccccatgt tcgcgtaagt gtcaggatat tgttcaacaa gttccggcat
      601 cacctgcgcc agcggtgtgt tggcgtcata gtggcgtttg aaggagcaaa gggtgttaac
      661 cagagtcccc catttcccac gggttacgcc catagagaac aggaacataa tttggaagtc
      721 agtggtgcgg gtaggcacaa tgccgtggcg accaagccag gcagtgacca gcgccgccgg
      781 aacaccggtt tcttccagtt caccatcttc acccattccc ggagcaagga tgctgacttt
      841 aatcgggtcg agcatactcc agttatccgg aatatctttg aagccgtgcc agctttcgcc
      901 cggatgcatt acccagcagt cctgaacggt ggtcagcagt ttggttggtg cgtcagcaaa
      961 gtcataggtt ttgccggttt gtgggtcggt gacgacttct ttgttccacg gtttgaagaa
     1021 ccagctaccg tcagcggcga actctttata cagccgcgcc atcgcctgac ggaaatcaac
     1081 cgcttcgtcg atcacttcct gcgtcagtga caggccgctg ttgccgtcca tcatcgacac
     1141 cgccacgtcg ttggatgcgc agatggcata cagcggggag gtggtggcat gcatcatgta
     1201 ggcctggttg aagcgggaga agttaatcgc cccacgacct tcacgtacat gaatataaga
     1261 ggcctgtgaa agcgcattca gcagtttgtg ggtggagtgg gtggcgaaaa cggtaggacc
     1321 gttgtgatcg ccaggttcgc cgcgcatggc atagtgatcg gcatagatcg ggttgaaacg
     1381 tgcatagccg taccaggctt cgtcaaagtg cagacgatcg gaggtttttt ccagcagatc
     1441 ctgcgcttct ttagcgttat aacacacgcc gtcataggtg cagttggtca ccacgcagta
     1501 agacggtttt tgcccggctt tgtctttagt cagcgggctt tcactgattt tcttctgcaa
     1561 ggtttcaggt tgcatttcct gcggatagat tggcccgata atgccgtagc ggttgcggct
     1621 tggcaccatg tagaccggtt tcgcgcctgt cagcatcaaa ccttgttcga tggatttatg
     1681 gcagttacgg tcaacgacca cgacatcgtt atcggtcatg caagcctgca tgatggtgcg
     1741 gttagagccg gaagtaccga cgactaccga ccaggagcga tcggcaccaa atacgcgtgc
     1801 ggcatatttt tcgctttcgc caaatgcgcc agtatggtca agcaaagaac cgagggaagt
     1861 tcgttcgatg cccatgtcgg tgcggaacag attttcaccg tagtagtcat ggtagaaacg
     1921 tccggcgggt gttttggtaa aaccaacgcc gccctggtgg cctggcgctg cccaggaata
     1981 ttcatggatg tcgctatatt tcatcagcgc gctgaacagt ggcggcaaca gctgctggcg
     2041 gtagcgggtc atcgcggcaa cggcgcgtcc ggcgataaag tcggcggtat cttccagaat
     2101 ccaggcgaat tcatcgacaa gctccagcag atcccggtcc attgcggcga gggctttttc
     2161 ccgatcgccc aacaggaaga ccggcacgtt ttgttggcgc tcatgaagct taccgatcaa
     2221 ttgtctgacg ttttgatgtt cgtccggatg ttccatttga tagctgaaca tcaggcagtc
     2281 aatggcttcg tttgaagaga gaatggcaaa accatcatca aaggaggtgg atttaatcac
     2341 ggtaacattt tgctggctta aagcatctgc cagacgctca acagcgttac cgacccaggt
     2401 gtcttgatgg agaaactcgc tttcaacaat taatactttc atcattgctt acccggttat
     2461 gaaggaatct tcgttgcggg caagtatctt ccggcctttt cgggctgtaa agcgcgtgaa
     2521 aaacaaacat ttttgaaata tttgttttta taaacaatat gttatgtgat tattttaaat
     2581 tattttcact gcttattatc gacggctaaa ctattttttt ggctgatact gatatcgtct
     2641 ttagccggga aacgtctggc ggcgctgttg gctaagtttg cggtattgtt gcggcgacat
     2701 gccgacatat ttgccgaacg tgctgtaaaa acgactactt gaacgaaagc ctgccgtcag
     2761 ggcaatatcg agaatacttt tatcggtatc gctcagtaac gcgcgaacgt ggttgatgcg
     2821 catcgcggta atgtactgtt tcatcgtcaa ttgcatgacc cgctggaata tccccattgc
     2881 atagttggcg ttaagtttga cgtgctcagc cacatcgttg atggtcagcg cctggtcata
     2941 gttttcggca ataaagccca gcatctggct aacataaaat tgcgcatggc gcgagacgct
     3001 gtttttgtgt gtgcgcgagg ttttattgac cagaatcggt tcccagccag agaggctaaa
     3061 tcgcttgagc atcaggccaa tttcatcaat ggcgagctgg cgaatttgct cattcggact
     3121 gtttaattcc tgctgccagc ggcgcacttc aaacgggcta agttgctgtg tagcaagtga
     3181 tttgatcacc atgccgtgag tgacgtggtt aatcaggtct ttatccagcg gccaggagag
     3241 aaacagatgc atcggcagat taaaaatcgc catgctctga caggttccgg tatctgttag
     3301 ttggtgcggt atacaggccc agaacagcgt gatatgtccc tgattgatat tcactttttc
     3361 attgttgatc aggtattcca catcgccatc gaaaggcaca ttcacttcga cctgaccatg
     3421 ccagtggctg gtgggcatga tatgcggtgc gcgaaactca atctccatcc gctggtattc
     3481 cgaatacagc gacagcgggc tgcgggtctg tttttcgtcg ctgctgcaca taaacgtatc
     3541 tgtattcatg gatggctctc tttcctggaa tatcagaatt atggcaggag tgagggagga
     3601 tgactgcgag tgggagcacg gttttcaccc tcttcccaga ggggcgaggg gagtgtccga
     3661 gcatcatgag gccgaaaact ctgcttttca ggtaaattat tcccataaac tcagatttac
     3721 tgctgcttca cgcaggatct gagtttatgg gaatgctcaa cctggaagcc ggaggttttc
     3781 tgcagattcg cctgccatga tgaagttatt caagcaagcc aggagatctg catgatgtct
     3841 gcacccaaaa ttacatttat cggcgctggt tcgacgattt tcgttaaaaa tattcttggt
     3901 gatgtgttcc atcgcgaggc gctgaaaacg gcgcatattg ccctgatgga cattgacccc
     3961 acccgcctgg aagagtcgca tattgtggtg cgtaagctga tggattcagc aggggccagc
     4021 ggcaaaatca cctgccacac ccaacagaaa gaagccttac aggatgccga ttttgtcgtg
     4081 gtggcatttc agattggcgg ttatgcacct tgcacggtga ctgatttcga ggtctgtaag
     4141 cggcatggtc tggaacaaac cattgccgat acgttggggc cgggcggtat tatgcgcgcg
     4201 ctacgtacca ttccgcatct gtggcaaatt tgcgaggaca tgacggaagt ctgccccgat
     4261 gccaccatgc tcaactatgt taacccaatg gcgatgaata cctgggcgat gtatgcccgc
     4321 tatccgcata tcaaacaggt cgggctgtgc cattcggtgc agggaacggc ggaagagctg
     4381 gcgcgtgacc tcaatatcga cccggctacg ctgcgttacc gttgcgcagg tatcaaccat
     4441 atggcgtttt acctggagct ggagcgcaaa accgccgacg gcagttacgt gaatctctac
     4501 ccggaactgc tggcggctta tgacgcaggg caggcaccga agccgaatat tcacggcaat
     4561 actcgctgcc agaatattgt gcgctatgaa atgttcaaaa agctgggcta tttcgtcacg
     4621 gaatcgtcag aacattttgc tgagtacaca ccgtggttta ttaagccagg tcgtgaggat
     4681 ttgattgagc gttataaagt accgctggat gagtacccga aacgctgcgt cgagcagctg
     4741 gcgaactggc ataaagagct ggaggagtat aaaaacgcct cccggattga tattaaaccg
     4801 tcacgggaat atgccagcac aatcatgaac gctatctgga cgggcgagcc gagtgtgatt
     4861 tacggcaacg tccgtaacga tggtttgatt gataacctgc cacaaggatg ttgcgtggaa
     4921 gtggcctgtc tggttgatgc taatggcatt cagccgacca aagtcggtac gctaccttcg
     4981 catctggccg ccctgatgca aaccaacatc aacgtacaga cgctgctgac cgaagctatt
     5041 cttacggaaa atcgcgaccg tgtttaccac gctgcgatga tggacccgca tactgccgcc
     5101 gtgctgggca ttgacgaaat atatgctctt gttgacgacc tgattgccgc ccacggcgac
     5161 tggctgccag gctggttgca ccgttaaaac gcgactaaac gctactgcgc cgggggattt
     5221 attccggcgc acacctctga cgataccaat aacagaaggc gggcgttggt aacagcgacc
     5281 cgatacccta tgagcatttc aatgactaca aaactcagtt atggatttgg agcgttcggg
     5341 aaggattttg cgatcggcat tgtgtatatg tacctcatgt attactacac cgatgtcgtc
     5401 gggctgtctg tgggtttggt cggtactttg tttctggtgg cgaggatctg ggatgctatt
     5461 aacgatccga ttatgggatg gattgtaaat gctacgcgat cgcgatgggg taagttcaaa
     5521 ccctggatcc tgatcggtac gttggcaaac tctgtaatct tatttctcct ctttagtgcg
     5581 catctgtttg aaggtactac tcagattgcc tttgtttgcg tgacctacat cctctggggc
     5641 atgacttaca ccattatgga tattcccttc tggtcgctgg ttccaaccat cacgctcgat
     5701 aaacgtgagc gtgaacaact ggttccttat ccgcgttttt ttgccagtct ggcgggcttt
     5761 gttacggcag gtgtgacgct accatttgtt aattatgtcg gcggtggcga tcggggattt
     5821 ggctttcaga tgttcacact ggtactgatc gcctttttta ttgtttcaac catcatcact
     5881 ctgcgcaatg tgcatgaagt cttttcgtca gacaatcaac cgtctgctga aggaagccat
     5941 ctgacactta aagccatcgt tgcgcttatt tataaaaacg atcagctttc atgcctcttg
     6001 ggtatggctc ttgcttataa tgtagccagc aacattatta ccggctttgc tatctattat
     6061 ttctcatatg ttatcggtga tgcggatttg ttcccctatt atctgtcgta tgcgggagct
     6121 gctaacctgg tgacgttagt attcttccca cgcttagtta aatcattatc ccgacgcatt
     6181 ttatgggccg gagcatctat tcttccggtg ttaagctgtg gtgttctcct gttaatggca
     6241 ttaatgagct atcacaacgt cgtcctcatt gtgattgcgg gtattttgct gaatgtggga
     6301 acggcgcttt tctgggtatt acaggtcatc atggtggcag ataccgttga ttacggtgaa
     6361 tataaactgc acgtacgctg tgaaagcatc gcttactccg tgcagactat ggtggtgaag
     6421 ggcggttcag cctttgcggc ttttttcatt gcggttgtgt tagggatgat tggctatgta
     6481 ccgaatgttg aacagtctac gcaagcccta ttaggtatgc agtttattat gattgctcta
     6541 ccgactctgt ttttcatggt aacgctgatt ctctacttcc gtttctatcg cctcaatggc
     6601 gacacgctgc gcaggatcca gattcatctg ctggataaat atcgcaaaat accgcccgag
     6661 cctgttcatg ctgatattcc ggtcggtgca gtgagtgatg tgaaagcctg acgtggacaa
     6721 tagctaacga aaatggcctg atgcgatatg tttatcaggc cacgtcttaa tggaatgctt
     6781 tggatttgca aattttgtag accggataag gaattcacgc cgcatccggc atcaacaaag
     6841 tgcacgttgt tatccggtta tcaagccaaa gcgccgtagc tgacggcaat gaaagcgagc
     6901 gaggaatatc accgtggtta atgcgccaag cagcgcgcag aacatatcgg attgcgtgtc
     6961 ccactggtcg ccctgggtgc cgagaaaatc atccgctccc tggcccattg ccagcgccgc
     7021 ccaccactcg attaattcat acatcgcgct tatcgccagc gccacgcaac agaccagaaa
     7081 cgccaccatt ttacgtccgc gaacgtacat cccgcgcaca aggatctctc gtgccaccag
     7141 tgcaggcacc agcccctgga aaaaatgccc cagcttgtca tacggattac ggctcaaccc
     7201 cagccattcc tgcacctcaa aaccgatggg gactttcgcg taggtgtatt gcccgccgac
     7261 catcaggatg atggcgtgaa agaaaatgag cgtatagagc agcggcgtta acggataacg
     7321 tttagcggtg gcaagcagta gcggcacgac aataatcacc ggtgtcactt ccatcagcca
     7381 ggtgagtttg tcatgggccg aaatgccggt ataaatcagg attaacgtta gcgccagtgc
     7441 gccggtgtta agaattaacg gcttgagtgt gcgggtcatg gtgagttcgc aagtcaggga
     7501 aaatactgac tattcaccag caagcgtgaa attacaatcg caaaagaggt ggcccagggg
     7561 gatcacctgg cagcatgctg ccaggcgctg ggccgaagag gttacttagt gcagttcgcg
     7621 cactgtttgt tgatgatttg ctggaagaag tcgttacctt tgtcatcgac caggataaac
     7681 gccgggaaat cttctacttc gattttccag atagcttcca tacccagctc cggataagcg
     7741 acgcattcca gatgcttgat gctctgctgc gccagtaccg ccgccggacc gccgatgcta
     7801 ccgaggtaga agccgccgtg tttatgacac gcgtcggtaa cctgctgact gcggttacct
     7861 ttcgctagca tgatcatgct gccgccgtgg gattgcagca gatccacata ggagtccata
     7921 cgacctgcgg tggttgggcc aagtgagcct gatggataac cggcaggggt tttcgccgga
     7981 cccgcgtagt agatcgggtg atctttgatg tactgcggaa gttctttacc ggcgtcaatc
     8041 agctctttca gcttggcgtg tgcaatatct cggcccacga taatggtgcc ggtgagcgac
     8101 aaacgagtgg aaaccggata ttgcgaaagc tgggcgagga tctctttcat cgggcggtta
     8161 aggtcgacct tcaccgcttc gccttcaccg gcctggcgca gttcttgtgg aatgtactga
     8221 cctggatggt gttccagttt ttcgatccag ataccttcgc ggttgatttt cgctttaatg
     8281 ttacggtcag cggagcagga gacgcccatg cctaccgggc aggatgcgcc gtgacgtggc
     8341 agacggataa cgcgaatgtc gtgcgcgaag tatttaccgc caaactgcgc gccaagaccg
     8401 agtttctggg ccttttccag cagttcctgt tccagctgga catcgcggaa cgcctgacca
     8461 tgttcgttcc cttccgtcgg cagttcatcg taatagtgag cgcttgctaa cttaacggtt
     8521 ttcaggttgg tttccacaga cgtaccgcca atcacaaacg cgatatggta cggcgggcag
     8581 gctgcagtac cgagggtacg cattttctcg acgaggaagt ttttcagttt gccgggagtc
     8641 agcagggctt tggtttcctg gtagagatag gttttgttgg cagagccgcc gcctttcgca
     8701 acgcaaagga atttgtactc atcgccatct accgcgtaca ggtcgatttg cgcaggcagg
     8761 ttagtgccgg tgttgacctc tttgtacatg tccagcgccg cgttttgcga gtagcgcagg
     8821 ttatcttcga tataggtgtt atagacgcct ttcgacagcg cttcttcatc accgccaccg
     8881 gtccacacgc gctggccttt tttaccgacg atgatcgcgg tgccggtatc ctggcaggtc
     8941 ggcagcacgc ctttggcggc gatttcggag tttcttaaga attgcagtgc cacgtacttg
     9001 tcgttttcgc tggcttctgg atcgtgaaga atagccgcaa cctgtttctg gtgtgccgga
     9061 cggagcatga aagaagcgtc gtggaaggct tgctgcgcca gcagggtcag ggcttctggt
     9121 tccactttca ggatggtttc gccgtcgaag tcggcaacgc taacgtaatc ggaagtgagt
     9181 agatagtatt cggtattgtc tttccccatc gggaaaggtg cctggtagat aaagggtttg
     9241 tttgacatag cttccagcct gtaactctgt atttgttatt cgaaaatggc gtgccgcgta
     9301 gtaacgggcg gcacgcaaag tgcatttata agaacccgta catcgcggcg aagatccagc
     9361 cgaagacgca cgatacgctc acaccaatca accccggcag aataaagctg tggttgatga
     9421 cgaagcgacc gatgtgggtg gtgccggaac ggtcaaactg aatcgctgcc agatcgctcg
     9481 gataagtcgg caggatgtaa taaccgtagc aagccggtgc tgaagccacg atgtatgccg
     9541 gatcaacgcc gatcgctagc gcgaccggaa caatcgccgc cagcgccgca gcctgagagt
     9601 ttacaaactt ggaaaccagc agcagaacaa tggcataggc ccacggatac tctttcacca
     9661 tttcacccag tacgccctga atttcagaca tatgcgcacc gaacatggtt tctgccatcc
     9721 atgcgatacc gtacaccgcc acgatggcga tcataccgga acggaagact tcgttttttg
     9781 agatagacgc gggattggtt ttggtcagga taataatcag cgccccggtc agcagcataa
     9841 acatctgaat aaccagtacc atcgacagcg gtttgccgcc gaaggatgga cgcaggtccg
     9901 aatcagcacc aagaagtgcg actacagcga ttgccccgag gaaaatccac attgccagcc
     9961 agttgctttt cggcagtttt ttatccagca gcgtcgcggt atcaccgtaa acatactcac
    10021 ggttttccgg tacggagatg aatttctgga actcttcgtc tttatccaga tctttaccgc
    10081 ggaaccagct gaagataccg atcgccagga taccgattaa cgtcgatgga atggtgattg
    10141 ccagcaggtc gaggaactca agatggcgac catcaaaggt gacattaccc agcattgcaa
    10201 ccagagacac gaccgcaacc gacaccggac tggcgataat ccccatctgt gcaccgatag
    10261 aacttgccgc catcggacgt tccggacgga tgttgttctt aatggcgacg tcgtagatga
    10321 tcggcagaat ggtgtaaacc acatgacccg taccgcaaag aatggtcagc gtacaggtca
    10381 caaacggcgc gacaattgag acatatttcg ggttgcggcg cagcagcttc tcggcaattt
    10441 gcagcatgac atcaagaccg cccgaagctt gcaaggtcgc cgatgccgcc accaccgcaa
    10501 tgataaccag catgacatca actggtggtt tacctggctg aaggtggaag acgaagacca
    10561 gaatgaccag accgataccg cctaataaac ccagcgcgat accacccttt ctggcaccat
    10621 aaaacagaca tatcagtatt atgataagtt ggatagtaaa taacatgtgt gaaccctcgc
    10681 gataatccta tttaaatttt tgctgaatag atcacagtca cgttctgttt tgtatgaact
    10741 gttttcagag cctgactaaa tatccgtttg gtcgttaccg gctttagcaa atacctcaca
    10801 gtgaatattg gctgattaaa gcagcggcaa atttatgcac tgtgataagc ggctttttca
    10861 gagagaaaaa ctctctggcg atgggtttaa agtttgtact gcgcagatgt taatgttttg
    10921 tttgctgtta tcttattgat atttatcggt ctattttttg ttctcagggc agatatcatt
    10981 aggtttatag attaatctga tctacccatt tgtgggtaaa aatacacata atgcgggtga
    11041 cataatagtt aattaacttt tgttagcgtt ttgaaattaa aaacaccgtt cacctgaaga
    11101 gatattaatt tttagcgatg atggagggat aattatattt gatctggcac aagttttact
    11161 gatgaaggat gtaacttgta ccaggggtat tttgcattac ggtaataatt attacttaca
    11221 gataaccagc gatccgttat tggcaatatt gtttcagtag tgagtagtgt tctgcctgaa
    11281 tacggtaacg gtaaactgga cgccccgtga cgccataatg gatactggtg aacaagatgt
    11341 ggcagttgac cagccagata aggtatttac ggcaggaaac acgcgaaatg ttaacctcgt
    11401 tggctagctc gtcggttgaa aactcatagt cctgatgcgc gtcaatccac tggcacagtg
    11461 tgcgtaacgt ctgcggcgtt aagccttttg gcaagcgacg aggatcctgc tcgttggagc
    11521 tgctgccgtg gattagctga tcaagctcgg cctggtcata atactgatgt ttttccagcg
    11581 ccattttctt ttgccgccag ccggtgagcg cctcttcaaa gcgggaagcc tggaagggtt
    11641 tgatcaggta atccacgaca ccgtaatgca gcgaatcttt aatggttgcc gcatcggctg
    11701 cggaggagat gacaatcaca tcacttttgc aacgcgcgtt atgcaggaca ggcagtaaat
    11761 cgagcccgtt ctctttttgc atatagatat cgagcaatat caggtcgata ggcgtatcgc
    11821 tattgaagat aatctctttg gctttctcca gcgtcgaggc tgttccacag cattgaaagc
    11881 ctgggatttg tgctacgtat cggcgattca gctccgcgac cattgcgtcg tcatcgataa
    11941 ttaatacatt gatcatctgt tcgacctctc cccgtcccag ggtatctgga caaaaaattg
    12001 tgtgaaaatc ccgggttccg attccacggc gatgctgccg ccgagatttt ctacctgttg
    12061 tttgacaagt gctaaaccga cgcctcgctc gcttcctttt gtcgagacac ctttgtcaaa
    12121 aatgtgatcg attttgtcgg gtgcgatccc cggtccatca tcgttaactt cgcagtgcag
    12181 ccagccgtga cggtagtgca atgttacgct aatttcgcca ccgggttccg gccctaatgc
    12241 ttccagcgcg ttttctatca gatttcccaa tgtggtaatc agcgtcgcga cctggtcctc
    12301 actgccgctg tctggcagct ggctttcact gtttaaaatc agcgtatggc ctaaatcggt
    12361 cgcgcggtta atcttgctga ttaaaaaacc agcgataacc ggagatttga tcttacccag
    12421 cagagagcca atctcttcct gatagttatt ggctgttttg agaatgtaat cttccaactg
    12481 cttataactc ttcagatgca ataatccgag aatcacatgc aatttattca taaattcgtg
    12541 ggatcgttca cgaagtgcat cagcatagtt gaccagaccg tcgagtcgct gcatcagttt
    12601 acgaacttca gttttgtccc tgaaggttga aatggcaccg atgataacgc cattactgcg
    12661 caccggaacg gtgttgatca gtaatagccg gtctttaatc gtaatctctt cgtcgcggcg
    12721 cggcgtaccg tcgcgtaaca cttccgagac atctaccacc tgtgaccatg agtggctgag
    12781 cgtcgacagt ttctcatcgt cctgcgactt acggtaattc agcaattctt gtgcggcatc
    12841 gttgatcagc gtgacctcgc cgcgatcgtc cacggcaacg acgccttctt tgatagactg
    12901 caacatggcc tggcgttgct caaacagcgt ggagatttcg tagggttcca ggccgaaaag
    12961 gatttttttc agtaccttaa ccagaatgca ggtgccaatc agtccgacca gcatgccaaa
    13021 taataccgac cagataatgc tccagcgact gtcattgatc tgttgggtca cgcggcttaa
    13081 ctcaaggccg atcgccacca cgccaatttg tttatgattt tcatcgtaga tgggggtaaa
    13141 tacgcgtaaa gcctgcgcca gaaaaccgcg attgatagcg acattttctt cgccattcag
    13201 cgctttaagg atgtcatcac ctttaaatgg ctgaccaata cgctgggctt caggatgcga
    13261 gtagcgaaga ctttgcatat cggtaacgac aataaacagc agatcgttgc gtttgcgcac
    13321 ggcttccgcg atggcctgga tgccgctctc ctgcggtttt ttctgcaagc cctgacggat
    13381 ttccggcgag tcggcgagag tacgcgccac tgccagtgcc ttgttggcta gcccgtctcg
    13441 cgtcatatca ctgatttgcg agaagtaaat cagatgcacc accaatagca ccgagaacag
    13501 taccgcgctg accattaaga tcactgtggt actcaatttc atcggacgtt tgcgtaacat
    13561 gcggtagggc aatgaatgtc tcatcagctt ccttgtgtga caaatttctt aagcattatc
    13621 tctgatgagg cgggtaattc aaagggagta agaatgattg gctatatagg ggaagagact
    13681 ctggcaacgg aaactgccag tgctgtatga agattccagg gctatgctta tagtgaaaac
    13741 catactgatg agagagggaa ggtcatggat caggcgctac tggacggggg ttatcgctgt
    13801 tataccggcg aaaagatcga tgtctatttc aacactgcga tatgtcagca ttctggcaat
    13861 tgcgtacgtg gcaacggcaa gttatttaat ctcaaacgaa agccgtggat catgccggat
    13921 gaagtcgacg ttgccactgt ggttaaagtg attgatacgt gcccgagtgg tgcgctgaaa
    13981 taccgtcata aataagcgag ggtaaaatgg aaatacgcga aggccacaat aaattttaca
    14041 ttaatgacga acaaggcaag caaatcgctg aaattgtctt tgtgccgacc ggagagaatt
    14101 tagcgattat cgaacatacc gatgtcgatg aaagcctgaa agggcaaggg attggtaaac
    14161 agctggttgc gaaagtcgtg gaaaaaatgc gtcgggaaaa acgaaaaatt atcccattat
    14221 gtccatttgc gaaacacgaa tttgataaaa cgcgggagta tgatgatatt cgcagttgat
    14281 ggaagagtac agattcacga tatttttcat gctctccgcg gtgtgatgca ggagagcatc
    14341 tgaagggtag ggggatgcac aaagaatggg cagagagcgc gtttttttgt cccaagtcat
    14401 cccctttact gagcaaaaaa aagaatatct cctatatgag aatcatcaat cggggttaat
    14461 aaattttgcg tccccagagt gttcaatatt tataggagtc atattatgga aggtaaaaac
    14521 aagttcaata cttatgttgt ttcttttgat tatccatcat cttattcctc agtgttctta
    14581 aggttaagat cattgatgta tgatatgaat ttctcttcta tcgtggctga tgaatatggg
    14641 ataccacgac aattgaatga aaattccttc gcaataacga catcgttagc cgcaagtgaa
    14701 atcgaagatt taatcaggct taaatgctta gacttaccgg atattgactt tgacctcaac
    14761 attatgacag ttgatgacta tttccgtcag ttttacaagt agccagtaag gataagaaga
    14821 acataaaaaa tggcactatt ctctaaaata ttaatttttt atgtgattgg tgtgaacata
    14881 tcctttgtca ttatctggtt tatctcacat gagaaaacac atattcgttt acttagtgca
    14941 ttcctggtcg gaataacctg gccaatgagt ctgcctgtgg cattactttt ttctctcttt
    15001 taggagctgt agttgagtgg acccgctggt tatcgtgaaa cgaagcgtct ctcctcaaaa
    15061 ctggatttgc ggaacgatga acgaagcagt cgcctgactg cttcgttcat taaagtgaaa
    15121 tttatttctg tgggcgcatc gccgggaaga gaataacgtc gcgaatagta tggctgttag
    15181 taaacagcat aatcattcgg tcgataccaa tacccagacc agcggttggc ggcagaccat
    15241 attccagcgc agtcacgtaa tcttcgtcat agaacatggc ttcgtcgtca cctgcggctt
    15301 tagcgttaac ctgttcctgg aaacgttcag cctgatcttc tgcgtcgttt aattcgctaa
    15361 aaccattacc gatttcacga ccaccgatga agaattcaaa acggtcggtg atttccgggt
    15421 taacatcatt acggcgtgcc agcggtgaca cttctgccgg atattccgta ataaaggttg
    15481 gctgaatcag atgtgcttct gccacttcat caaagatctc cgtgacaata cgtcccagcc
    15541 cccagctttt ctctaccgta ataccgatag attcagctaa tgctttagca gcatcaaaat
    15601 tatccaggtc ggccatatcg gtttctggac gatatttttt gattgcttcg cgcatggtga
    15661 gtttttcaaa cggtttgccg aaatcaaaca catgctcgcc ataagtgact ttagtggtac
    15721 ccagaacctc ttgtgccagg gtgcggaaca gggactctgt cagttcaatc aaatcgtggt
    15781 aatccgcata cgccatgtag agttccatca ttgtgaactc aggattatgg cgaacagaaa
    15841 taccttcatt acggaagtta cggttgattt cgaatacccg ttcaaaaccg cctacaacca
    15901 gacgtttcag atacagctcc ggcgcgatac gcagatacat atctaaatcc agagcattat
    15961 gatgggtaat aaacgggcga gcagatgccc cacctggaat tacctgcatc atcggggttt
    16021 ctacttccat aaagccgcgc gcgaccatga actggcggat agcggccaga atttttgaac
    16081 ggacaacaaa cgtttgacgg gatttatcgt tagcgatgag gtccagataa cgttgacgat
    16141 aacggacttc ctgatcctgc agaccatgga atttatctgg taaaggacgt agtgctttag
    16201 tcagcaggcg caactcagta cagtgaatgg aaagctcacc cgtttgcgtc ttaaacagcg
    16261 taccgcgggc accgataatg tcacccagat cccatttttt aaactgatcg ttataaacac
    16321 cttctggcag gctatctcgt gcgacgtaca gttgaatacg gccaccgaca tcctgcaacg
    16381 ttacaaagga ggctttcccc atgatacgac gggtcatcat tcggccagca accgagactt
    16441 caatgtttaa ggattccagt tcctggttat ccttcgcatc aaactcttcg tgcaactggt
    16501 cagaggtatg gtcgcggcga aaatcattgg gaaacgccac accttgctga cgtagtgccg
    16561 ccagtttttc gcggcgattt ctcagttcat cgttaaaatc aatagcctca ttggctcccc
    16621 gtgtttcttg ttcagacatt ttggttcctc taaatccagc tttcaattta gcgtagataa
    16681 agagacagat cggtacactg tattatctgc ctcgactatt aataactcag tttatcttat
    16741 cgaaactaac gcttacctca agttgatatc actaataaat aaccatcaaa atggtttaaa
    16801 tgacttatta atctcgattt actagtgtga taaactgaaa gttaatttat tcataaccaa
    16861 ttgtttttac gacagttaat cgttgctctc ctgtaccata ttcgtcggcg tgctaaacag
    16921 gaaacgggta gcaaaggcca ataccacgat gatcgcgaca caagccaacg tccactctcc
    16981 catctgagaa aagaatcgct ggtaagctgc aattgccata ccgctaattt gcgactctgt
    17041 cgtctgctgt gccactacgc ctgccagcca gttggcgacc gcgcctgttg ccagcatata
    17101 aatcccggtt aatacgccag acattttcag acgcgtgatt tgcgcaatcg ccaccgggtc
    17161 aataaagagt tcggcaaagc ccattagcgc cagcccggat accatcacgc ccattgacgc
    17221 ttgaccgtca gctgctgcat gtcgggcatc aaatgccagc aacataaagc cacaagccat
    17281 cagcagtaag ccaaaggcaa acttcagcca gacgcgcaat gttgagttgc cgtggctttc
    17341 tggcgacgcc agccaggcca gaacaacccc agcgagcatc accgcaattg cattcaccga
    17401 ctggaatagt gcagtaggta cttcaatatt gaatgcctga cgattcacaa agcgatcgat
    17461 aaacaagctg atggtactgc cgccctgttg tgccagtacc cagaacaatg tcccgacaaa
    17521 catcaacaat acaatttgcc aaagagcacg gcggtgttcg gggaatttga tcatcatgcg
    17581 ggcaatgatt tgtgcggcaa tgaggcaaac gatcgccagc aaatatccgg accagtcgtt
    17641 ctccagcagc agggtaaaaa atactggggc taaacagagc atcaccacta accagctcca
    17701 taccggtaag gcaaatttga cgctagtgag cgctttttta tccatactac gcgtggactg
    17761 gaaatgacga tgcccgctta agaaaatcaa caaaccgata aacatgccgc cacccgcaag
    17821 ggcaaagcca acatgccatc cataccactg agcagccagg ccgcaggcga ttggggcggc
    17881 gatagaaccg atattgcccg cagcatacag cagcgaaaaa ccgccatcac gtcgatgatc
    17941 gttctcgtcg tagagctcgc caagcaaaca gctgatgttt gatttgaata aaccgtagcc
    18001 acaaataatg attgccagcg ccagatacag gctaaaggtt gaatttgtat caatacccag
    18061 caccacatgg ccaagggtca ttaacagcgc gccggcaatc actgcagtgc ggttgccgag
    18121 caggcggtcg gcaagccagc cgccgagaat aggggtaacg taaaccagag aagcatatgc
    18181 gctgaacagg ctgatggcat ggttatcatc aaaaccaagc tgatgggtga gatagagaat
    18241 gagtaaggca cgcatgccgt aaaaactgaa gtactcccag atttggatcg ccacgatata
    18301 gtatatcgcg cgcggctgtg agggtgtttt catgtgttct ccttatgagc aaaaaaggga
    18361 agtggcaagc cacttccctt gtacgagcta attatttttt gctttcttct ttcaatacct
    18421 taacggtata gcggccatca gcctgacggt atgcaccgtg aatatcggtt tcaaagcccg
    18481 gatagtgagc gccgatttca cacagcatct gcaggaactc cagaaccgga cggctttctt
    18541 cggtgatcat ttcacccggc attaccagag gaactcccgg cggatacgga aggatcatat
    18601 tggcgttaat acgaccgacc atttcgtcga ggtaaacttc ttcggtcata ccgtgcagct
    18661 ctttctggaa tgcagcatac ggagtcatta ccatcgtcgg cagcacttca aatgcgcgat
    18721 acatcagatc cggcagattg tggtgaacaa tcagtttgtg gatattctga gccagttcct
    18781 gaatacgcat gttttcatag aattcaggat cttcacgata cagagacggc agcatgtttt
    18841 tcacacgcag gttcaggtcg aacgcacgtt tgaagtcagt cagagcacgc agcaggctca
    18901 gtgctttggt cttatcgata ccgatgctga acaggaacag caggttatac ggaccggttt
    18961 tctcaacaac gatgccatgt tcgtcgaggt atttcgccac gatgctggcc ggaataccaa
    19021 agtcgctcat ggtgccgtct ttttccatcc ccggagtcag cagggtgact ttgatcgggt
    19081 caagatacat gtgctcgtta tcgatgtttt tgaagccgtg ccaggtgctg tcagaacgca
    19141 gcggccagca ttcagtcgta tcgatatgat ccggctgcca tacatcaaag aaccagccat
    19201 cagattccgt tctcagacgt ttgatctctt tacggaattt gatcgcacgt tcaatggaac
    19261 cgttgatcag acgcttacct gcattgcctt tcatcatcgc cgcagcggtt tcagtggacg
    19321 ccacgatacc gtagtgcgga gaagtggtgg tgtgcatcat gtaggcttcg ttaaaggttt
    19381 cttcgtttac gtcaccttta acgtggatca tggaagcctg agagaacgcc gccagcagtt
    19441 tgtgagtgga ctgggtttcg taaatcactt tcccttctac acggccaccg ctcataccgc
    19501 atttaccttc gtaaatcggt gagaagttgg tgtaaggcac ccacgcggag tcaaagtgga
    19561 tggatttcac atccagtgtt ttcttgatga agtcggtgtt gtacagcaga ccatcatagg
    19621 tagagttggt aattacagca tgtaccggcc aggttgcgtt tggtgtttct ttcacgcgct
    19681 tagcaatggt agcgtgctgg aattcactct gtgggatacc accaagaata ccgtaagcgt
    19741 tacgggtcgg gcggaaatag attggcgtaa catcgctcat catcatcagg tgggtcagcg
    19801 atttgtggca gttacggtca atcagaatgg tgctgcctgc cggagcagag tacataccaa
    19861 caattttgtt cgcagtggaa gtaccgttgg tcaccatgta gctgcggtct gcgttaaaga
    19921 cgcgagcgat atactgttct gcttctttgt gtggaccact gtgatccagc agagaaccca
    19981 gttcagatac tgaaatggaa atatcagatt tcatggtatt cggaccaaag aaatcataga
    20041 acaggctacc taccgggctt ttctggaatg cagtaccacc catgtgacca ggagtacaga
    20101 aagtatattt accttcacga acatatttaa acagtgcttt agtcagcgga ggcagaatag
    20161 tgttgatata ttcgtcagtg gtctgcttga tcttgttagc aatatcttca gcagcaccca
    20221 gcgcatattc aaagaagcta atctgtaaac gcaggtcatt caggcttaca tcgagagtgg
    20281 aatacgtatt agcgaacgcg tacaacggca ggttctcgtt cattttgcta atttcttcgc
    20341 acagctcgag attatattta tcccagtcaa aaataacgcc gcacagacgc gcattgtttt
    20401 cgatcagttt taataagtcg tcacggtcgt tcgggtaaac aatctggaag ttcagacgtt
    20461 caagcgcgcg atgaagttca cggatgggtt cttctttaaa ataaaccccc atgtgattca
    20521 atattgcaat aacgttcata gtcatatctc caggtaaaaa aggcccctcc caacacatgg
    20581 gacaaaatga aaggaggagc ctcggaaaat acttttaatt aatgtgcgtt agacgcggtg
    20641 tggttatcca ttgagtggct ctggcgctcg tgcattttgc gagcgtagaa catcaggata
    20701 atcaggctga cgatgaaggt acctgccagc tcgaaggagc ttgcgcccat cagcgcgatg
    20761 aagcagaaca cgcaacccag tacagagcag atcaggctga caaagttgcg gatgttaacg
    20821 ccttcaaaac gaatcaggtc aacgcaagag tagaagtacg gcagcatagt cagcagtact
    20881 gcgataccgg tcagttcacc gaacaggtca gatgctttac caccggcaga gttcatcaga
    20941 gtgatgagga tcatcagggc agtcattttc actgcagcca gcagcagacc ttttttcgga
    21001 ataccgttgc tgtcgacttc accataaact ttcgggaagt taccgtcgtt agcggcacgt
    21061 acacctgcct ggcctaccaa catcatccag gagcccagag aagtcaggca cgcaaaggcg
    21121 gtgaatgcag aaaccagcgg cgcagcccag ttaccgagga tagttgaagc actgattgca
    21181 aacggagcac cggaagccgc cattacagaa gacggataca taccggaaag cacctgagtc
    21241 gcagcgatgt aaacaatacc tgctaaacca gtacccagca tggttgccag cggaacggta
    21301 cgtttcgggt ttttaaccat accagtactt acagctgcgg attcaacacc cacgaaggcc
    21361 cacaggcaga gcagaatact tttaatgatc gcatgaccat cagtggtatc cgcagtattc
    21421 cagttagctg cataagttgc cgcatcaaac caatgccagc caacaatagc agtcatcacc
    21481 acaggaataa gaaccagcac cagaccaata gtggttaaac ggcttaccca ggtaccgccg
    21541 agcatattta caaaggtaaa tacccagacg atagcaatac aggcgatacc cgccggaaca
    21601 ggatcattta atactgggaa gaaggtggaa agataagata cagcggtaat accaatcgcc
    21661 aggttaccaa tccagttagc atggtaataa agaacacctg tctgaaaacc aaatgcagga
    21721 gaaatttctc cggcataagc aattgggcca ccttgttgcg ggttttttgt tgccagtcgg
    21781 gcatatacat acgccagcga cattgcacca ataatagaga taatccaacc ccagatagca
    21841 ataccaccga tacttgctag gttcgcaggt aataatgcaa taccgctccc catcatatta
    21901 ccggcaacaa caccggtaca ggcaaatagc ccgatcttct tggcagaact catgctcttc
    21961 tcctaatttc atttttgaat ttggagtccg ggtcatgatg tataactatt tcctgaccag
    22021 accaaactgg cgctaagatt actcacgaaa aaaggattaa tcctaaagat taggtgaaat
    22081 aacacaaaag tttctgtaag tgagaacttg aggtttttta ttaacacatc aggatcgcaa
    22141 gttgatatca tgaaaagata aacattcaat gtttacaata gattgcgtga cgtcttctgg
    22201 ttaaatttat gtaataaaaa ttatgcggca aataaattgc cgcaacatat tataccaaca
    22261 ggaacataca aaaactcaac aacaaatatt cccgagcatg aatcaacccg ggggttactt
    22321 attctgaagc gagaaatttg tcgagataag gtacaacata aggaacagaa gtctggaata
    22381 taccattttc aatccagtaa agggtgtttg cccctgggcg taaattaaag gcggtgagat
    22441 atgcatcagc cgcttcccgg ttcatcccct tcatttcata aaccttgcca agcaatacat
    22501 aatttagcca ggacatttca agatcaatgc cagtatttat cgcctggtaa gactcatctg
    22561 ttttaccttt taccagagca ctgaccgctt ttatttgata tataatggac aggttattca
    22621 attccggtag tgtaacaatg ttatctattt ctgtgttcag tgctgctaat tgtttttcat
    22681 ctaaaggatg ttgagaatgg cgcacgatat caactaatgc tttttctgct ctcgcgtagg
    22741 taaattctgg ggatgattga acaatctcac ctaataattc actggcacgg ttcaatgatt
    22801 tatcatcgcc atgcagtaaa taatcatgtg cctgataaaa attagttaat aacgcaccac
    22861 gatgaggcaa aatttgctgg agcgtctcct gcattcgttg tggccacggt tggtttaacg
    22921 cttttgataa actctccagt aaatcatttt gaatcgccag ctgattaccg ttagtgatga
    22981 cataacgttt atccagcatg gttgaaccat ctgcattgtc taccaatttt atcgacataa
    23041 agcattgttg agcacggtat tggcgctgat taacaaacgc aatagataat gttttaccgg
    23101 aactgctcgg ttcatcaatg ttgtagttga ttttgtcatg caccataaag gtggagaagg
    23161 tgttaagtga tgtcgccacc aaatcaccca cgcctatcgc gtaagagagc tgatacgggg
    23221 aactccagct gttacaactt ttatttacca tattaatgtc aatatcgcgt ggattgagca
    23281 aaatacgcga tttgctcatc ggaagacgtg tatcaagact tgaaaacgct accagtgcta
    23341 cacagatacc taaggacaac aggaaaaaaa accataccca aaaggtagtg aatcgtttgc
    23401 ttttaactgg ggattgttca ggtggcgttg cggtgttttg aatgttaaga ctgtgggagg
    23461 gagaatctgt ggcaggaacc gcctctggta tagggggagg cgaagatagc attatttcct
    23521 ctccctcttc ttcgctgtac cagataaccg gcaccattaa tttatagccg cgctttggta
    23581 cagtagcgat atagacagga ctatcttcat cattatcttt taatgactta cgtagttctg
    23641 agatactctg cgtcacaacg tgattggtga caatacttct cttccagaca ttatcgataa
    23701 gttcatccct gctaagtact tcgccactgt gttgagcaaa gaaaaccaga aggtcgatta
    23761 atctcggctc aagggtaagt tgacgcccat tgcggctaat ttggtttatg gacggagtaa
    23821 caagccattc gccaacgcga actacaggtt gttgcataat agaaactcat tcgaaaaggg
    23881 aatgatgcaa tgataattgc cacaacctat ttttaccatc tatagatggg tttatttaca
    23941 tattattggt gaatgcaaga cgttattttt accagccata aacttcttga ttacatggta
    24001 ttacgaaagg attttactga gaaccagaag taatattcct taccatcaaa attcatcatc
    24061 tttgccaaag aaaaatgttc agaaaataat ccatggaaaa ttgtccggag cgcttactat
    24121 tttaatggat tgttagtctt tgcatgagca agcggactga tacatttctc tttgttctca
    24181 ttcagaaaat ctcatcagtc ggcgttcaga ccgatgttgc gctttatgga tgcgttacgc
    24241 tcctgatgac gtcatttgac gttcaacagc atcacggggc cgcacgacat ttcacgttag
    24301 ttagtgctat agctcaggaa caaattttcc cgaattggga tatgcccgca aattgctggt
    24361 gatgtgggga gaatcgggtt gagttcggta gaattgattt ggaggcagaa cacttaaatc
    24421 gtggcgtcct gaaacgaaaa acggacctcc gtggaggtcc gtttatatga atttggtgcc
    24481 cggactcgga attgaaccaa ggacacgggg attttcaatc ccctgctcta ccgactgagc
    24541 tatccgggca acggggcgca ttaaaccgta atctgcacat ctcgtcaacc taatttcagt
    24601 aaaagcgatt caactgctta agattgcggc aaatcgcttc ttttctgtgt ttctgtcagg
    24661 tcagtgcacc accctggcgg cagcgggcga agcgcaggat atcttccgcc agccgatgtg
    24721 cggtgtcgac atccgcctgg ctacgattca ccagcattcg gcttaaacag ccttccagca
    24781 ccagttccat ctgctttgct accatcgccg gatcgtcaac ttccagcgtg gttaacagtt
    24841 cgtgggtgaa atcgtaggcc gcgctttttt gctgatcggc cagttgatga atagggtggc
    24901 caggatcggg ataaaacgta caggcagcga taaacagaca gcccggatag cggttgtttt
    24961 taacgcactc cgataacgcc tgataacgtg ccagcagctt ttgttcggcg gtttgcgttt
    25021 cgtccagcat cagctgacga cgccagacat ctatctgttg gctaagataa cgcagcgcat
    25081 cgtagaggat tgcctctttg tctggccaga agcggcgtag ctcgtccagt ggataatcca
    25141 cacgttcagc aaccatctcc agcgtggtgt tggcaatccc ttgtaattct aataatttca
    25201 gggcttctcc cagtacatct tcacgttgca cgctattttc ctccgtcttt cccactgcaa
    25261 gtgtcgttca cggttggcga tcgcgcaaat gtgcgctgaa ggtttcagca tccataaagc
    25321 ccgtgacgcg tgcttgtgga tgctcctggc cttgtccgtc aaaaaagaga attgtcggta
    25381 ggccaaggac attaagatgc tttaacagcg ccacatcttg tgcgtcgttg gcagtgacgt
    25441 tggcctgaag taataccgtg tctgctaacg ctttttgcac ctgcgggtcg ctgaaggtgt
    25501 atttctcaaa ctctttacag gcgacgcacc agtcggcata aagatctaac atcaccggtt
    25561 tgcctttggc ttcaacgagc gcctgattta actcatctac cgttttgatt tgtgtaaagt
    25621 tgagatgcgt ctgagtttgc gcggtatgcg tcgcaccaaa tgcccaatcc tgaagtgggc
    25681 gcacgctaac caatgccgct gcaagcagga ttatttgcac cacgcgcatc cagccgcgtt
    25741 tggcctgtag gctggtgata aaggcccagc caaagaatgc gacacccagc gccgaccaca
    25801 agcgtaatcc ccatacatca ccaatcactc gctccagcag gaagaccggc agtgcgagga
    25861 tcacaaaacc aaacgcggtt ttgacttgtt ccatccacgg gccgcttttc ggcagcaagc
    25921 ggttaccaaa gacggtaatt agcatcagcg gcaggcccat gcccaacgca tagagataaa
    25981 gcgtgccgcc gcccagccac atgttcccgc tttgggcgat atacagcaga atcgcgctaa
    26041 gcggtgcggt ggtgcatggt gaacagatca gtccggcaat cgtccccata ataaacactc
    26101 cgccgggtga gccgccctgt tggcgattgc tcatcagcgt gaggcgcgtt tgcagcgagg
    26161 aggggagttg tagagtaagc aagccaaaca ttgacatcgc cagcaaggta aagacgatgg
    26221 tgaggccaat gagcacgtat gggtgctgta gcgccgcctg gaactgtaac cctgcggcgg
    26281 caactaccag acccagcgcc gtgtaagtca gtgccatccc ctgcacataa ataaaggtca
    26341 gcaacaatgc tctggcagtg gagagccgct gtttaccacc cagcacgatg ccagaaatca
    26401 gtgggtacat tggcagcacg catggcgtaa aggcgatacc aataccgatc aacaacgccc
    26461 agagcgcgga aaagggcaat tgcgcggtgg gctgctcttg ctgcggaaca gacacaggct
    26521 gtgacgctgc gttgttggcg accacttcgc ttaacggaac ggttttggtt tctggcggat
    26581 aacagaaacc ggcatcagca cagccctggt aggtgacagt taacgtcgct cccgcactcg
    26641 cctggttgat ggttacggga agcgtcagcc gatcgcggta aatctcgctt ttgccgtaaa
    26701 actcatcttc atgccagacg ccttgcggca gctgcacgtc ggcaattttc gcgtgttccg
    26761 gcgtaatgcg gatctgttta cggtagaggt agtaaccgtc tttgatctgc caggtcagat
    26821 taaggtcatg ttggttttgc tgaaaatcaa aagcaaaggc ttgatccacg gggacaaatt
    26881 gtgaacgtcc cggcgcgtcg aataatccgg caaaaacgga agtgctgcaa agtagcagga
    26941 tcagcgtaaa gatgcgttga gccatgagag gtaatctgtg tctccgtgtg taacaggtaa
    27001 aaccagaagt tccggggttt gatatggatg atgagacttc aggcattcca gcagtgcctg
    27061 ctggtgagat acggtagttt ttaaaatcat ctgcacttcg tattcttgct ccagcttacc
    27121 ttcccagtaa tagagagagg tagcgccggg gatcaaggtc gcgcaggccg ccagtttttc
    27181 cgccagcact ttggcggcta aatcctgggc tgtcgcttca tccggtgcgg tacatagcac
    27241 cacgacagac gcggtattcg aacttttttc atcaagcata aacacctcgc aagaacagat
    27301 ggaaccgcaa gagagaggtc actatacaac ggacggggaa aggatgttag tcggcgagaa
    27361 agaaagcggg gcgtgaacgc cccgcgattg gtcattacag catgaagcta cccagcacga
    27421 agccgaagca aacggccagg gcaacaccca gagtacccgg gatgaagaac ggatggttga
    27481 agacgaattt accgatacgg gtagtacccg tgtcatccat ctgtaccgca gcaaccagcg
    27541 tcgggtaggt cggcagaatg aacagaccag acaccgcagc gaaagaagca acagcggtca
    27601 gcggagaaac gttcagtgcc agagccatcg gcatcagtgc ttttgcggtt gcagcctgag
    27661 agtacagcag agcagaagca aagaagaaga tgacggccag cagccacgga tgaccctgaa
    27721 tcacttcacc agcggtatct ttgatccagt cgatgttgtt ggaaacgaaa gtatcgccca
    27781 gccacgcaac acccaggata caaatacagg cgctcatacc tgctttgaag gtgctggagt
    27841 tgaggatgtt gtcggtatcg actttacaga taacggtggt cagagttgca acactgagca
    27901 tgatgatcag gattgcgttg gtggtgttca tcagcggttt ttcaaccaga cccatgcttg
    27961 ggctgttgat gattgcatag ataaccacgc caactacgcc cagcaggaac agccagacgg
    28021 acgtttttgc accggatttg atttcaatct gcttttcacc gcgcagttca accaggccct
    28081 cttccagacg cttgcgataa atcggatcgt cagagagttt ggagttgaac agcatagtga
    28141 ccaggaagga catcaccaga accgccagca gggtggacgg gatgaccacg gagagcagat
    28201 ggaggtagct gatgccatga ccttccatca cggaagacat gtaaaccact gccgctgaga
    28261 ttggcgatgc ggtgatcgca atctgcgcgg ataccactgc agtagacagc ggacggcaag
    28321 gtttaacgcc ttgttccttc gcaacttcag cgataactgg cagtgtcgcc agagagatgt
    28381 tgccagtacc agcaaagata gtcaggaaat aggtcacgat cggtgcgagg atcgtgatgt
    28441 atttcgggtt acggcgcagc agcttttctg tctgatgaac cagatagtcc agaccgccag
    28501 caacctgcat ggcagaaata gcggcgataa ccgccatgat aatggaaatg acatcgaacg
    28561 ggatgttacc gggtttaacg ccaatagcgg caagaaccag cacccccaat ccgcctgcaa
    28621 aaccaatacc tattcccccc aatctggcgc ccaagaagat cgccagcaaa actatgatga
    28681 gttctacaac tagcatatta gccttccttg ttttttaaca agttgatatt agattgttat
    28741 ttttaagtta ctgctcacaa gaaaaaaggc acgtcatctg acgtgccttt tttatttgta
    28801 ctaccctgta cgattactgt tcgctttcat cagtatagcg ttttgctttg taagccgggt
    28861 gcatcagatt ctgtacggag aaaatatcgt caagttccgc ttcagtcaac agaccgcgtt
    28921 ccagaacgac ttcacgtaca ctcttaccgg tttcggcaca gattttaccc acgatgtcac
    28981 cgttgtggtg accgatgaac gggttcaggt aagtaacgat accgatagag ttgtaaacgt
    29041 aaccttcgca cacttctttg ttagcagtga tgccgttaat gcatttttcc agcaggttgt
    29101 agcaagcgtt ggtcagaatg tgaacggatt cgaacatagc ctggccaatg accggctcca
    29161 taacgttcaa ctgcagctga cctgcttctg ctgccatggt aacagtggtg tcgttaccga
    29221 tgactttgaa gcatacctgg ttaaccactt ccggaacaac cgggtttact ttagctggca
    29281 tgatggaaga gcccgcctgc agttccggca ggttgatctc gttcaggccg gcacgtggac
    29341 cagaagagag caagcgcagg tcgttacaga ttttggacat cttcacagcc aggcgtttca
    29401 gcgctccgtg aaccataaca taagcgccgc agtcagaggt cgcttcgatc aggtcttcag
    29461 ccggtacgca tgggaagcca gtaacttcag ccagtttttt cactgccagc ggagagtact
    29521 ctttcggcgt gttcagacca gtaccgattg ctgttgcgcc aaggttaact tccagcagca
    29581 gttcagcggt acgttggatg tttttcactt cttctttcag caggatgctg aaagcgcgga
    29641 attcctgacc gagggtcatc ggtactgcgt cctgcagctg ggtacgaccc attttcagga
    29701 tgtcctggaa ttcgaccgct ttacgttcaa agccttcacg caattggtta atcgcatcta
    29761 ccagcttaat cagggaagag taaactgcga tacggaaacc ggtcgggtag gcgtcgttag
    29821 tggactgaca tttgttaaca tggtcgttcg ggttcaggta ctgatattca cctttctggt
    29881 gacccatcag ttccagaccg atattggcca gcacttcgtt ggtgttcatg tttacggaag
    29941 tacctgcgcc gccctggtag acgtctaccg ggaactgatc catgcatttt ccgttgttca
    30001 ggacttcatc acatgcggca atgatggcat tcgctacact tttaggaatg gtttgcagct
    30061 ctttgtttgc catagctgcg gcttttttaa ccattaccat accgcgaaca aattcaggaa
    30121 tatcactgat tttgttgttg ctgatataga agttttcaat cgctctcaga gtgtgaacac
    30181 catagtaggc atcagctgga acttccctgg tacccaacag atcttcttcg atacgaatgt
    30241 tgtttgacat gtgaaccttc tttttcaagc tgccaatgat ttgctttaaa cacacagaat
    30301 atatgtggtt tcgaatgttt ttcgaccgac gattatcccc tgcatcgacc gaatatccga
    30361 gatcatatgc tgcttgagga tttctaccgt aatctggatc actttaagtg tcggttttta
    30421 ccccttaatt attaatttgt gaaatagatc accgctttgg gattactacc aaaaatagtt
    30481 gcgcaaatat cttgaaattt tgctaatgac cacaatataa gctgaacgcg attcgcaacc
    30541 cattcaggta gccgggttta accggctgct attacaggag aaacctttgc gctggttacc
    30601 ttttattgcc attttccttt atgtctatat tgagatttca atctttattc aggttgccca
    30661 tgtattgggg gtattgctga ctctcgtgct ggttatattt acgtcagtta tcggtatgtc
    30721 actggtacgt aaccagggct ttaagaattt cgtgctgatg cagcaaaaaa tggcggcggg
    30781 tgaaaaccct gcggcggaga tgattaaaag tgtttcgctg atcattgccg gtttgctgct
    30841 tttattaccg ggctttttca ccgacttcct cggtcttctg cttttattgc cgccggtgca
    30901 aaagcatctg acagtgaagt tgatgccgca tttgcgcttt tctcgcatgc caggcggcgg
    30961 ttttagcgcc gggaccggtg gcggtaatac ttttgatggt gagtaccagc gaaaggatga
    31021 tgaccgcgac cgccttgatc ataaagacga tcgccaggat taatgtcgaa acgccggatt
    31081 atgtggttat gccattttcc ggcgtttttc gttttggcag aaacagccat aaccccgcca
    31141 gcatgatcag cgcatagaga cttttccagc cgaccattgc cagtaacaga acgcataaca
    31201 gcccgccaac caccgccagt agtcgataac gaccttgcaa taatttacag cctgccagca
    31261 tgcataacag ataaatcata ataaagatgc cattggcata aataataaga gcgtccagat
    31321 tgatctctaa agcatgaatc accaacgtgc tcaccacgca gcagccgagc accgcattga
    31381 gggcattatt cgggatatgg cgagaagaga ggcgtgccag gtagtggtca ggattatgtt
    31441 gcgcctgcga ccagaccagg cgggcgaagc tctgtatata aatgttgaga ctggcaaagc
    31501 aggccagata gccaattacg caggcaatcc ataacgctcc tacaccgaac agttgtacta
    31561 caatttttgg aagcgatgct gccgcggcca tttgttcacc ataggcgtcg aagtgtaaga
    31621 cgactaccgt acagccccag tagactaatc ctgccagcag cagaccaatc atcaaagcac
    31681 gaggaaaatc acgttctgga tttttaaatt ccgaggcaag atgggcaaat gcttccagac
    31741 cgacaaaaca ccagaacatc actgataacg cagcaaataa cccggtaagt tcgatatttc
    31801 ctggcgcggg gaaggggata ctcgcaggtt tgatatcgcc cgcccaccag atagcgacaa
    31861 tcagtgcgac gataagtccg gcaataactg tttgtagatt tgcactggaa ctggcaccgc
    31921 gagtaccgat ataccacacc aacgccagcg taccgagttc tgccaacaac agttgccagc
    31981 tatgccagcc aaacattgcc tggccgaatc cggcagcaat ttgtagcgcg gcaggcaaac
    32041 ccacgggaat gaccgataaa aacagccagc cggtgactcg ctcaagccgc gaaccaaacg
    32101 ccataccgac gaagtgcgcg acgccgcctg cgctgggata gtggcgaccc agaatcgcaa
    32161 acacaatcgc aatcgggaac actaagataa tcaaaatggg ccacgcccac aggctgttat
    32221 tgcccgctac cagtgcagct aacgcaggaa cggcaaacac gccagtgcct aataatgacg
    32281 tcgatagcag gccaatgccc tgggccagcc ccagttcttg tttgagtcca ctcatgggtt
    32341 gatgtccgat tgcgcccaaa ttttgggcaa ctgcgtagat tttcgatggt agcacaatca
    32401 gattcgctta tgacggcgat gaagaaattg cgatgaaatg tgaggtgaat cagggttttc
    32461 acccgatttt gtgctgatca gaattttttt tctttttccc ccttgaaggg gcgaagcctc
    32521 atccccattt ctctggtcac cagccgggaa accacgtaag ctccggcgtc acccataaca
    32581 gatacggact ttctcaaagg agagttatca atgaatattc gtccattgca tgatcgcgtg
    32641 atcgtcaagc gtaaagaagt tgaaactaaa tctgctggcg gcatcgttct gaccggctct
    32701 gcagcggcta aatccacccg cggcgaagtg ctggctgtcg gcaatggccg tatccttgaa
    32761 aatggcgaag tgaagccgct ggatgtgaaa gttggcgaca tcgttatttt caacgatggc
    32821 tacggtgtga aatccgagaa gatcgacaat gaagaagtgt tgatcatgtc cgaaagcgac
    32881 attctggcaa ttgttgaagc gtaatcctcg cacgacactg aacatacgaa tttaaggaat
    32941 aaagataatg gcagctaaag acgtaaaatt cggtaacgac gctcgtgtga aaatgctgcg
    33001 cggcgtaaac gtactggcag atgcagtgaa agttaccctc ggtccgaaag gccgtaacgt
    33061 agttctggat aaatctttcg gtgcaccgac catcaccaaa gatggtgttt ccgttgctcg
    33121 tgaaatcgaa ctggaagaca agttcgaaaa catgggtgcg cagatggtga aagaagttgc
    33181 ctctaaagcg aacgacgctg caggcgacgg taccaccact gcaaccgtac tggctcaggc
    33241 tatcatcact gaaggtctga aagctgttgc tgcgggcatg aacccgatgg acctgaaacg
    33301 tggtatcgac aaagctgtta ccgctgcagt tgaagaactg aaagcgctgt ccgtaccgtg
    33361 ctctgactct aaagcgattg ctcaggttgg tactatctcc gctaactccg acgaaaccgt
    33421 aggtaaactg atcgctgaag cgatggacaa agtcggtaaa gaaggcgtta tcaccgttga
    33481 agacggtacc ggtctgcagg acgaactgga cgtggttgaa ggtatgcagt tcgaccgtgg
    33541 ctacctgtct ccttacttca tcaacaagcc ggaaactggc gcagtagaac tggaaagccc
    33601 gttcatcctg ctggctgaca agaaaatctc caacatccgc gaaatgctgc cggttctgga
    33661 agccgttgcc aaagcaggca aaccgctgct gatcatcgct gaagatgtag aaggcgaagc
    33721 gctggcaact ctggttgtta acaccatgcg tggcatcgtg aaagttgctg cagttaaagc
    33781 tccgggcttc ggcgatcgtc gtaaagctat gctgcaggat atcgcaaccc tgactggcgg
    33841 taccgtaatc tctgaagaga tcggtatgga gctggaaaaa gcaaccctgg aagacctggg
    33901 tcaggctaaa cgcgttgtga tcaacaaaga caccaccacc atcatcgatg gcgtgggcga
    33961 agaagctgca atccagggcc gtgttgctca gatccgtcag cagattgaag aagcaacttc
    34021 tgactacgac cgtgaaaaac tgcaggagcg cgtagcgaaa ctggcaggcg gcgttgcagt
    34081 tatcaaagta ggtgctgcta ccgaagttga aatgaaagag aaaaaagcac gcgttgaaga
    34141 cgccctgcac gcgacccgtg ctgcggtaga agaaggcgtg gttgctggtg gtggtgttgc
    34201 gctgatccgc gtagcgtcta aactggctga cctgcgtggt cagaacgaag accagaacgt
    34261 gggtatcaaa gttgcactgc gtgcaatgga agctccgctg cgtcagatcg tcctgaactg
    34321 cggcgaagaa ccgtctgttg ttgctaacac cgttaaaggc ggcgacggca actacggtta
    34381 caacgcagca accgaagaat acggcaacat gatcgacatg ggtatcctgg acccaaccaa
    34441 agtaacccgt tctgctctgc agtacgcggc ttctgtggct ggcctgatga tcaccaccga
    34501 atgcatggtt accgacctgc cgaaaaacga tgcagctgac ttaggcgctg ctggcggcat
    34561 gggtggcatg ggtggcatgg gcggcatgat gtaattgccc tgcacctcgc agaaataaac
    34621 aaacccccgg gcagaaatgt ctgggggttt ttcttttggt catctttcta gtataagatt
    34681 cagacacgga cgacgcgagt ggcgtccagc tcattgatta tggggaataa catgcacgta
    34741 aaatacttag cagggattgt cggtgccgcg ctactgatgg cgggttgtag ctccagcaac
    34801 gaattgagtg ctgccggtca gagtgtacgc attgtggacg agcagccagg cgcagagtgc
    34861 cagctgattg gtactgcgac aggtaagcaa agcaactggc tttccgggca acacggagaa
    34921 gagggcggtt ctatgcgcgg cgcagcaaac gatctgcgca accaggcggc tgcaatgggc
    34981 ggtaacgtga tttatggcat cagtagcccg tcgcagggaa tgttgtccag ttttgtcccg
    35041 acggatagcc agattatcgg ccaggtatat aagtgcccga actgatgtga tggtgacaaa
    35101 cgtataattg ccagatgcgc ttcgcttatc aggcctacat aaggcattgc aactgattga
    35161 atgcttgaga atttgtaggc cggataaggc gtttacgccg catccggcat gaccaacgat
    35221 cacgttatca gtcatttcag gctggatggc tccagcctcg cattagcaaa gatgagatta
    35281 tttcgcctgt ggtgcagttt tgctggtgga ttgaacacgt aagtgatgca gcgcgcgtaa
    35341 gcattgatcc tgggttagcg gttgggcgtt attcgccgct acggtgcgaa catagacctg
    35401 acatggcaca cccacgagtt ttttcagtcg tgggctgaaa tcaagcattc ggcgagtgat
    35461 cgcctctatt tctttacccg gcgttgcttc acgcgttttc accactgcgc cgctaaattg
    35521 ttctttgcca ctttcatctt taaagcggta aagcaccgcc agataatggt cgaacaagtt
    35581 atgtttccat tccggttggg aataggtgtc atattccaga ttgccatttt gctggcagtc
    35641 aacgtcatat aacggcagga aatctaacag tgaggtaata cgtaatgcca gttcacgcat
    35701 ttccgcatta ttaattgcat gaatgattgc tcgcgccagc atttcaatct gcaactcatt
    35761 gacgactaac tcacatttgc taccgtcatg tagcgttaat acgaaggtaa gattttcgcc
    35821 ttgattatcg gtaagttcca gcgtgttaac ccgacgatta atatcggcat ttttaagctc
    35881 atcaaccaga atttctggga tgttttctgc cattttctta atgactttat cgcgagcttg
    35941 ctcatactga aggcgtgcag cagcatccag tttatgcttt tgatgcagac gactttccag
    36001 cgcaatcagc aggtcgcgca gttccatgac agacataaag aacaacgatt ctttattgcg
    36061 aggttcttta attttcagcg ccagtgcaat gaaattatta ctcttgcgaa taacaccggt
    36121 attaacaccc ttgatgctta
//
LOCUS       DAZFDC010000033        34752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFDC010000033 DAZFDC010000000
VERSION     DAZFDC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.033"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..746
                     /gene="elfD"
                     /locus_tag="ACHJ48_001770"
     CDS             45..746
                     /gene="elfD"
                     /locus_tag="ACHJ48_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4707343.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKLVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            771..3370
                     /gene="elfC"
                     /locus_tag="ACHJ48_001771"
                     /pseudo
     CDS             771..3370
                     /gene="elfC"
                     /locus_tag="ACHJ48_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3361..4431
                     /gene="elfG"
                     /locus_tag="ACHJ48_001772"
                     /pseudo
     CDS             3361..4431
                     /gene="elfG"
                     /locus_tag="ACHJ48_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4459..4986
                     /gene="ycbU"
                     /locus_tag="ACHJ48_001773"
     CDS             4459..4986
                     /gene="ycbU"
                     /locus_tag="ACHJ48_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4707344.1"
                     /translation="MFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDVATKSA
                     LQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAPTLLKL
                     TDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKSTKAGAT
                     GGNATAVLYFDLVYQ"
     gene            4994..5509
                     /gene="ycbV"
                     /locus_tag="ACHJ48_001774"
     CDS             4994..5509
                     /gene="ycbV"
                     /locus_tag="ACHJ48_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4707345.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5475..6211
                     /gene="ycbF"
                     /locus_tag="ACHJ48_001775"
                     /pseudo
     CDS             5475..6211
                     /gene="ycbF"
                     /locus_tag="ACHJ48_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6322..7332
                     /gene="pyrD"
                     /locus_tag="ACHJ48_001776"
     CDS             6322..7332
                     /gene="pyrD"
                     /locus_tag="ACHJ48_001776"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO4707346.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7506..8048
                     /gene="zapC"
                     /locus_tag="ACHJ48_001777"
     CDS             7506..8048
                     /gene="zapC"
                     /locus_tag="ACHJ48_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO4707347.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8045..9154)
                     /gene="ycbX"
                     /locus_tag="ACHJ48_001778"
     CDS             complement(8045..9154)
                     /gene="ycbX"
                     /locus_tag="ACHJ48_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO4707348.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDSSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9398..11506
                     /gene="rlmKL"
                     /locus_tag="ACHJ48_001779"
     CDS             9398..11506
                     /gene="rlmKL"
                     /locus_tag="ACHJ48_001779"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO4707349.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKEPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11518..13425
                     /gene="uup"
                     /locus_tag="ACHJ48_001780"
     CDS             11518..13425
                     /gene="uup"
                     /locus_tag="ACHJ48_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4707350.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEPAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13555..14808
                     /gene="pqiA"
                     /locus_tag="ACHJ48_001781"
     CDS             13555..14808
                     /gene="pqiA"
                     /locus_tag="ACHJ48_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO4707351.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14813..16453
                     /gene="pqiB"
                     /locus_tag="ACHJ48_001782"
     CDS             14813..16453
                     /gene="pqiB"
                     /locus_tag="ACHJ48_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO4707352.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     VLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16450..17013
                     /gene="pqiC"
                     /locus_tag="ACHJ48_001783"
     CDS             16450..17013
                     /gene="pqiC"
                     /locus_tag="ACHJ48_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO4707353.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17269..17436
                     /gene="rmf"
                     /locus_tag="ACHJ48_001784"
     CDS             17269..17436
                     /gene="rmf"
                     /locus_tag="ACHJ48_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO4707354.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17506..18024)
                     /gene="fabA"
                     /locus_tag="ACHJ48_001785"
     CDS             complement(17506..18024)
                     /gene="fabA"
                     /locus_tag="ACHJ48_001785"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO4707355.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18093..19853)
                     /gene="ycbZ"
                     /locus_tag="ACHJ48_001786"
     CDS             complement(18093..19853)
                     /gene="ycbZ"
                     /locus_tag="ACHJ48_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO4707356.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20039..20491
                     /gene="matP"
                     /locus_tag="ACHJ48_001787"
     CDS             20039..20491
                     /gene="matP"
                     /locus_tag="ACHJ48_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO4707357.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20567..21607)
                     /gene="ompA"
                     /locus_tag="ACHJ48_001788"
     CDS             complement(20567..21607)
                     /gene="ompA"
                     /locus_tag="ACHJ48_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO4707358.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21964..22473)
                     /gene="sulA"
                     /locus_tag="ACHJ48_001789"
     CDS             complement(21964..22473)
                     /gene="sulA"
                     /locus_tag="ACHJ48_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO4707359.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22692..23321
                     /gene="sxy"
                     /locus_tag="ACHJ48_001790"
     CDS             22692..23321
                     /gene="sxy"
                     /locus_tag="ACHJ48_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO4707360.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKYPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23284..25446)
                     /gene="yccS"
                     /locus_tag="ACHJ48_001791"
     CDS             complement(23284..25446)
                     /gene="yccS"
                     /locus_tag="ACHJ48_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO4707361.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25456..25902)
                     /gene="yccF"
                     /locus_tag="ACHJ48_001792"
     CDS             complement(25456..25902)
                     /gene="yccF"
                     /locus_tag="ACHJ48_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO4707362.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIALIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26025..28079
                     /gene="helD"
                     /locus_tag="ACHJ48_001793"
     CDS             26025..28079
                     /gene="helD"
                     /locus_tag="ACHJ48_001793"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO4707363.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28111..28569)
                     /gene="mgsA"
                     /locus_tag="ACHJ48_001794"
     CDS             complement(28111..28569)
                     /gene="mgsA"
                     /locus_tag="ACHJ48_001794"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO4707364.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28665..29327)
                     /gene="yccT"
                     /locus_tag="ACHJ48_001795"
     CDS             complement(28665..29327)
                     /gene="yccT"
                     /locus_tag="ACHJ48_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO4707365.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29500..29913
                     /gene="yccU"
                     /locus_tag="ACHJ48_001796"
     CDS             29500..29913
                     /gene="yccU"
                     /locus_tag="ACHJ48_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO4707366.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29958..30275)
                     /gene="hspQ"
                     /locus_tag="ACHJ48_001797"
     CDS             complement(29958..30275)
                     /gene="hspQ"
                     /locus_tag="ACHJ48_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO4707367.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30333..31523)
                     /gene="rlmI"
                     /locus_tag="ACHJ48_001798"
     CDS             complement(30333..31523)
                     /gene="rlmI"
                     /locus_tag="ACHJ48_001798"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO4707368.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31618..31896
                     /gene="yccX"
                     /locus_tag="ACHJ48_001799"
     CDS             31618..31896
                     /gene="yccX"
                     /locus_tag="ACHJ48_001799"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO4707369.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31893..32222)
                     /gene="tusE"
                     /locus_tag="ACHJ48_001800"
     CDS             complement(31893..32222)
                     /gene="tusE"
                     /locus_tag="ACHJ48_001800"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO4707370.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32313..32972)
                     /gene="yccA"
                     /locus_tag="ACHJ48_001801"
     CDS             complement(32313..32972)
                     /gene="yccA"
                     /locus_tag="ACHJ48_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO4707371.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKMANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33380..34399)
                     /locus_tag="ACHJ48_001802"
     CDS             complement(33380..34399)
                     /locus_tag="ACHJ48_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4707372.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(34377..34619)
                     /locus_tag="ACHJ48_001803"
     CDS             complement(34377..34619)
                     /locus_tag="ACHJ48_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO4707373.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
BASE COUNT         8921 a         8296 c         9027 g         8508 t
ORIGIN      
        1 agtgttatgg aggggatatt catatttttt aagagtgact atttatgaaa acttgcataa
       61 caaagggaat tgtgaccgta agtttaacgg caatattact cagctgttcg tccacatggg
      121 cggcaggtaa aggcggggtt ggacttgcag caacacgtct tgtttattcg gaaggtgaag
      181 agcaaatttc actgggtgta cgtaacacca gtccggatgt tccttatctg attcagtcat
      241 gggtgatgac cccagataat aaaaaatcag cggactttat tattacacca ccgctatttg
      301 tgctgaatcc ggcaaacgag aatctgttac gcattatgta cattggcgcg ccgttggcga
      361 aagacagaga aactcttttc ttcactagcg tacgggcagt cccttcaaca acgaagcggg
      421 aagaaggcaa taccctgaag attgccacac aaagcgtcat caaacttttc tggcgaccaa
      481 aaggtttagc gtatccctta ggcgaggctc cggcgaaact gcgttgcact tcgtcagctg
      541 acatggttac ggtcagtaac ccaacacctt atttcattac cctgacagac ctgaaaatag
      601 gtggaaagtt agttaaaaat caaatgattt ccccctttga taaataccaa ttctctctgc
      661 caaagggtgc caaaaatagc agcgtaacgt atcgaaccat caatgactac ggggcggaaa
      721 cgccgcaact caactgtaaa tcgtaattcg tcttcagtta agagagcgag atgtatagaa
      781 ctcaccgaca acacagcctg ttaagctctg gtggagtgcc atcgtttatt ggtgggctgg
      841 tggtgtttgt gtcggcagcg ttcaatgcac aagctgaaac ctggttcgat ccagcctttt
      901 tcaaagatga tccctcaatg gtggccgatt tgtctcgttt cgaaaaagga caaaaaataa
      961 cgccaggggt ttatcgtgtc gatattgttc tgaatcagac aattgtagat acgcgcaacg
     1021 tcaattttgt tgagataacg ccagagaagg ggattgccgc ctgtttgacg actgaaagcc
     1081 tggatgcaat gggcgtgaat actgatgcat ttccggcttt taaacaactg gacaaacaag
     1141 cgtgtgcgcc attggcggag attattccgg atgccagcgt aacttttaat gtgaataaac
     1201 tccgtctgga aatttcagta ccgcaaattg ccatcaaaag taatgctcgt ggttatgtcc
     1261 ccctgaacgt tgggatgaag ggatcaacgc gctattactg ggatattcat ttagcggggc
     1321 taacagtatt catagcagcg cagacagtga ttctggcgac agttattttc tgaatttaaa
     1381 cagtggcgtt aatttaggcc catggagatt gcgcaacaat tcaacatgga gtcgtagtag
     1441 tggccaaacc gcagaatgga agaatctcag cagctatttg caacgggcgg ttattcctct
     1501 gaaaggcgaa ctgaccgtag gtgatgatta tactgcaggc gattttttcg atagtgtcag
     1561 ctttcgtggt gtgcagctgg cgtcagatga caacatgctg ccagacagcc tgaaagggtt
     1621 tgcgcctgtg gtgcgtggta tcgccaaaag caatgcccag ataacgatta agcaaaatgg
     1681 ttacaccatt taccaaactt atgtatcgcc tggtgctttt gaaattagtg atatctattc
     1741 cacgtcgtcg agcggtgatt tgttagttga aatcaaagaa gcggacggca gcgtcaatag
     1801 ctacagcgta cccttttcca gcgtgccatt actccagcgt caggggcgaa tcaaatacgc
     1861 ggtgacactg gcgaaataca gaaccaatag taatgaacag caggagagca aatttgccca
     1921 ggccacgttg cagtggggcg gaccgtgggg aacgacctgg tatggtggtg gacaatatgc
     1981 tgaatattac cgtgccgcca tgtttggtct gggatttaac ctcggcgatt tcggagcaat
     2041 ttcgttcgat gtgacccagg cgaagagtac gctggcagac caaagcgaac ataaaggtca
     2101 gtcatatcgt tttctgtatg ccaaaacgct caaccaattg ggtactaact ttcaattgat
     2161 gggctatcgc tattcgacgt cgggtttcta caccctttcc gacaccatgt ataaacatat
     2221 ggatggctac gaatttaatg acggtgatga tgaagatacg ccgatgtggt cgcgttatta
     2281 caatttgttt tacaccaaac gtggcaaact gcaggtcaat atctcccagc aattaagcga
     2341 gtacggttcg ttttatttaa gtggtagcca gcaaacttac tggcataccg atcaacagga
     2401 tcggctatta cagtttggct ataacacgca aattaaagat ctctcgctgg ggatctcctg
     2461 gaactacagt aagtcccgtg gccaacctga tgctgatcag gtgtttgcac ttaatttttc
     2521 cctgccactc aatctgttgc tctccagaag taatgatagc tataccagca aaaaaaatta
     2581 cgcctggatg acctctaaca ccagtatcga taacgaaggg cacactacac aaaacctggg
     2641 tttaacggag acactactcg atgacggtaa cctgagctac agcgtgcaac agggatataa
     2701 cagcgagggg aaaacggcta atggcagcgc cagcatggac tacaaagggg tgtttgcaga
     2761 tgcccgagtg ggctacaact acagcgataa cggcagtcaa caacaactga actacgctct
     2821 ttcaggcagt ttagttgccc attcacaggg cattaccctg gggcaatcac tgggggaaac
     2881 taacgttctg attgcagcac caggcgcaga gaatactcgt gtggcgaaca gcaccgggct
     2941 gaaaactgac tggcgcggat ataccgttgt tccttatgcc acttcttatc gtgaaaatcg
     3001 aatcgcactt gatgcggcgt cgttaaaacg taacgtggat cttgaaaatg cagtagtcaa
     3061 cgtggttccc accaaagggg cgttggttct ggcggagttc aatgcccatg cgggagcaag
     3121 ggtattaatg aaaacatcaa agcagggtat atcgctgcgt tttggcgcga tagcgacgct
     3181 ggacggcgta cagactaata gcggcataat tgatgatgat ggctcgctct atatggcggg
     3241 tttaccggcg aagggaacaa taaccgtgcg ctggggcgaa gctcccgatc aaatttgtca
     3301 tatcagttac gagcttaccg aacaacaaat taactctgcg attacgcgaa tggatgccat
     3361 atgcagataa tctttggaga aaaatgcgtg tcattactac gactattttt tgccgccgtc
     3421 ttaatgctat ggtgcgctca aaccgctgct tatagcgggt agtgtcatac cactcagggg
     3481 aatccgtata ttggcgtcaa ttttggcgtt aaaaccctgg aggaagaaga aaatacgact
     3541 ggggtagtaa aagacaaatt ttatcagtgg aacgaatcga atgattatta tgtttcctgt
     3601 gattgcgata aagacaatgt cagaagtggc cgatgggcat tcgccgcgga ttcaccgtta
     3661 gtctatttag gcgacaactg gtacaaaatt aatgactatc ttgccgccaa agttttattg
     3721 caggttaaag gcagttctcc tacagcggtt cctttcgaaa acgtggggac tggggcagat
     3781 acccggtggc atatttgtga ccccggcggt caacgtttag gcggccaggg agctagcggt
     3841 aatagcggta gcttttccct gaaaatattg cagccgttcg ttggttcggt cgtcattcct
     3901 cctatggcgc tggcgcgatt atttgaatgc tacaacatac ccgcaggtga ttcctgcacg
     3961 actacaggca caccggtttt agtgtattac ctgtctggta ctatcaattc acttggctca
     4021 tgttccgtca atgccggaga aacaatcgag gtcgatctgg gcgacgtatt tgcggctaac
     4081 tttcgtgttg tagggcataa gcctcttggg gccagaacgg cagaacttgc aattccagtc
     4141 aggtgtaaca cgggaaacgc ggggttagtt aacgtcaacc tgagtctgac ggcaaccaca
     4201 gaccccagct atccccaggc gattaagacg tcacgtcctg gcgtgggcgt ggtggtgacc
     4261 gatagccaga acaacattat ttcccctgct ggtggaacat taccgctctc tattcctgat
     4321 gatgcagaca gtatcgcgtg aatgaatgtc tatccagtca gcacgacagg tgtaccacca
     4381 gaaaccgggc gatttgaagc cacggcaacg gtgagaataa attttgatta acgcgtgaac
     4441 gtatgaagaa aaaaaacgat atttcagtgc gttatcttgt tctttagcat tcttaacatc
     4501 catgtcggga tggctgggcc tgaacaagtt agtatgcata tttatgggaa tgtggtcgat
     4561 cagggctgtg atgtcgccac caaaagtgca ttacaaaata ttcatattgg tgattttaat
     4621 atcagtgatt ttcaggccgc gaataccgta agcactgctg ctgatttgaa tatcgatatc
     4681 accggttgtg ccgctggtat tactggcgcg gacgtccttt ttagcggcga ggctgacacc
     4741 cttgcgccga cactgctcaa actaactgac acaggcggaa gcggtggtat ggcaacgggg
     4801 attgccgtgc aaattcttga tgcgcaaagt cagcaagaaa tcccgctcaa tcaggtccag
     4861 cctcttacgc ccttaaaagc cggggataac acactcaaat atcaacttcg ttataagtcc
     4921 acaaaggcgg gagcaacggg cggtaatgcg acggcggttc tctattttga tctggtttac
     4981 cagtgaagga gtgatgttga aacggataat ctggattctg ttcttactgg gattaacggg
     5041 ggggtgtgag ctatttgccc atgatggcac ggtcaacatt agcggatcgt ttcgccgtaa
     5101 tacatgcgtg ctggcacagg atagcaagca aatcaatgtg cagttaggcg atgtctcact
     5161 gacacgtttt tctcatggga attatggccc ggagaaatct ttcatcatta atttgcagga
     5221 ttgcggaaca gatgtcagca cggttgacgt caccttttca ggaacccccg acggagtgca
     5281 gagcgaaatg ttgtccattg aaagtggtac ggatgcagcc agcgggctgg caattgcgat
     5341 tctggatgat gcgaagatat tgattccgct taaccaggcg agtaaagact acagcctaca
     5401 tagcggtaag gtgccattaa ctttttatgc gcaattgcga cctgtcaata gcgatgtgca
     5461 gtcgggtaaa gtgaatgcca gcgcgacatt tgtacttcat tatgactaat acgtggaaca
     5521 gattggcgct cttgattttc gccgttttat cgttgctggt ggcgggtgaa ttgcaggcag
     5581 gagtggtggt cggcggaacg cgatttatct ttccggcaga cagagaatcg atatctattt
     5641 tactgactaa taccagtcag gaatcctggc ttattaaccg taaaatcaac cgcccaacgc
     5701 gttgggccgg gggtgaagcg tcgacagtgc cagcaccatt actggccgct ccgccactta
     5761 ttctcctgaa gcccggtacg acaggcacgt tgcgcttgct gagaacggaa agcgacatct
     5821 tgcctgtgga tcgcgaaacg ctatttgagt taagcattgc cagcgtgcca tccggcaaag
     5881 ttgaaaatca gagcgtaaaa gtggcgatgc gctcggtatt taaactgttc tggcgacccg
     5941 aaggttgccg ggcgacccgc tggaagctta tcaacaatta cgctggacac ggaagtcgca
     6001 gggtgtacaa ctcactaacc caacgcctta ttacattaac ctgattcagg tgagcgtaaa
     6061 tggtaaagcg ttaagtaatg tgggagttgt gccgcctaaa agccagcgtc agacaagctg
     6121 gtgtcaggca atagcaccgt gtcatgttgc ctggcgggcg ataaatgatt acggcgggtt
     6181 gagtgcaaag aaggagcaaa atctgccctg aaacaggttc ggaaaacgtt tgcgtttttt
     6241 ttgccgcagg tcaattccct tttggtccga actcgcacat aatacgcccc cggtttgcac
     6301 accgggaatc caggagagtt catgtactac cccttcgttc gtaaagccct tttccagctc
     6361 gatccagagc gcgctcatga gtttactttt cagcaattac gccgcattac cggaacaccg
     6421 tttgaagcac tggtgcggca gaaagtgcct gcaaaacctg ttaactgcat gggcctgacg
     6481 tttaaaaatc cgcttggtct ggcagccggt cttgacaaag acggggagtg cattgatgcg
     6541 ttaggcgcga tgggatttgg atcgatagag atcggtaccg tcacgccacg tccacagcca
     6601 ggtaatgaca agccgcgtct ctttcgtctg gtagatgccg aaggtttgat caaccgtatg
     6661 ggctttaata atcttggcgt tgataacctc gtagagaacg taaaaaaagc ccattatgac
     6721 ggcgtcctgg gtattaacat cggcaaaaat aaagatacgc cagtagagca gggcaaagat
     6781 gactatctga tttgtatgga aaaaatctat gcctatgcgg gatatatcgc catcaatatt
     6841 tcatcgccaa ataccccagg attacgcaca ctgcaatatg gtgaagcgct ggatgatctc
     6901 ttaactgcga ttaaaaataa acaaaatgat ttgcaagcga tgcaccataa atatgtgccg
     6961 atcgcagtga agatcgcgcc ggatctttct gaagaagaat tgatccaggt tgccgatagt
     7021 ttagttcgcc ataatattga tggcgttatt gcaaccaata ccacactcga tcgttctctg
     7081 gttcagggaa tgaaaaattg cgatcaaacc ggtggcttaa gtggtcgtcc gcttcagtta
     7141 aaaagcaccg aaattattcg ccgcttgtca ctggaattaa acggtcgctt accgatcatc
     7201 ggtgttggcg gcatcgactc ggttatcgct gcgcgtgaaa agattgctgc gggtgcctca
     7261 ctggtgcaaa tttattctgg ttttattttt aaaggtccgc cgctgattaa agaaatcgtt
     7321 acccatatct aattatttct tcgaattcct ctttataaga taatcagggc tttattttca
     7381 gccctggttg ttttatattc attgctgttg cttatttaga cattttgtac ttttattgtt
     7441 gaggttatta agcgaagcga caatggattg tgttgctgcg gttttatagg gtaggggaga
     7501 ggcagatgcg aattaaacca gacgataact ggcgttggta ttacgatgaa gagcacgatc
     7561 gtatgatgct cgatttagcc aatggtatgc tatttcgctc acgttttgcg cgcaaaatgt
     7621 tgactccaga tgccttttct cccgcaggct tttgcgttga cgacgccgcg ctctatttct
     7681 cttttgaaga aaagtgccgc gattttaatt tatctaaaga acaaaaagcc gaactggtgc
     7741 tgaatgcact ggtagcaatt cgctatctca aaccgcaaat gccgaaaagc tggcattttg
     7801 tttcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc ggcctgcgtt tggttgagcg
     7861 acactaacga gcaagtgaat ttactggtcg ttgaatctgg cgagaatgcc gcgctatgcc
     7921 tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat gcagttgggc gacgccatta
     7981 aaatcatgaa cgacaggctg aaaccgcagg ttaatgttga cagcttcagc ctcgaacagg
     8041 cagtctaacg cgccaactta agtgcagtct tcggtacaca gctacagcaa agaatggtgc
     8101 catcatcgcc cattgctgat tttttcagcg gcgtgacttc gccttctaaa agctgaacac
     8161 ggcaacttcc acaaatgccc gcgcggcaag aataagggat acgaattccc tgattttcta
     8221 attgctccag caacacctgt tggttatttc cacgaaatgc ctgtccctgc cagtcaatat
     8281 ctacattggc gtccggttgt tgcgtgatgt tggcagtatc atcagcggca gctgcgccgt
     8341 aaatttttgc cggagccgtt gtcagaattt ccacctcatc gccaacgcga atcacgccgc
     8401 tattacgggc aattaaattc tgaccaaaat cgacatcgcc gttatcctgg gcagtgcgga
     8461 aagattgcaa tgtttttaat ggttcgcctg ccggatgttt ttgccctttt tctgggctga
     8521 cggtggtgaa aatacagcgg ctacaaggtt taaccacatc aaacaccaca tcaccaatgc
     8581 gaatcacttt ccaactatct tcttcccagg ctgacgcgcc ggaaaccacc agattggggc
     8641 ggaattgctc catttttaca ctggccggac aacgttgttg gagatcacgt aacgaggctt
     8701 cgttagcaag aaggtaagga tagccatcag caaatgacag gggtacagtg ttgtggcgtt
     8761 tcacgcgccg ggtcatttgt ggccccaccc agcgtaattg cacttcgcgg gagaaaaatc
     8821 cacttaacca tttgttgatc gcttctggcg caattcgcgc ggtaaaatgt gtgccccaaa
     8881 cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg aacatatgcg ctactgctat
     8941 ctggtgcggt gagatgcaag ccatcatgca cgggtgaagg agtaaatcgt accatctggg
     9001 gaaactggcg agcggtgata aacgtaccgt caggttccgt gatcataaag atgcgatcga
     9061 aggccagacc actgacatct gccagagcat gtgtaagacc aatgccgcgc atcgatttaa
     9121 caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg gtcctcaaat gaaaataagc
     9181 cctcaacttt atgacatgac gcgcttatta gctataatgc gcaacaattt tcttagcgcc
     9241 tgtcccatta ggctatttta tttgtcagtt tggccctggg ttgtgctcga aatcctcacg
     9301 tacttgtgta cgctccggtt tctccgcgct gtccatgtcc agactgactg caacaattac
     9361 gcctactgcg gtaggctctt agagtaaaag tgacgatatg aattctctgt ttgccagtac
     9421 ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa aacctggggg ccgttgaatg
     9481 ccaggtggtt cagggtgggg tccatttcaa gggcgacaca cggcttgttt accagagcct
     9541 gatgtggagc cgcctggcct cgcgtattat gttgccgctg ggcgagtgta aggtttacag
     9601 cgatttagac ctctatctcg gtgttcaggc gatcaactgg acagagatgt ttaatcctgg
     9661 cgcgaccttc gctgtccact tcagtggttt gaatgacact atccgcaaca gtcagtacgg
     9721 cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg cggaaaaatc tgccgcgtcc
     9781 aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc tggctgcata aagaaaccgc
     9841 cagtatcgcc cttgatctca gtggtgatgg tttacatctg cgtggctatc gcgatcgtgc
     9901 tggtattgcg ccgatcaaag aaaccctggc agccgctatt gtgatgcgat ccggctggca
     9961 gccaggaaca ccgttgctcg atccgatgtg tggttccggt acgttgctga ttgaagcggc
    10021 gatgctggcg accgatcgcg caccaggctt gcaccgtggg cgttggggct ttagcggctg
    10081 ggcgcagcat gatgaagcta tctggcagga agtgaaagcg gaagcgcaaa ctcgcgcccg
    10141 taaaggcctg gctgagtata gctctcattt ttacggttcg gacagcgacg ctcgggtgat
    10201 tcaacgtgca cgcactaacg cccgtcttgc ggggattggt gaactgatca cctttgaggt
    10261 gaaagatgtc gcgcaactga ccaatccgct gccgaaagag ccgtacggca cagtgttaag
    10321 caacccgcca tacggggaac gtctggacag cgaaccggcg ctgattgcgc tgcatagcct
    10381 gctgggtcgg atcatgaaaa accagtttgg tggctggaat ctctctttgt ttagtgcctc
    10441 gccggatctg ctaagctgct tgcagctgcg tgcagacaaa cagtacaagg cgaaaaacgg
    10501 cccgctggac tgcgtacaga aaaattacca cgttgccgaa agcactccag acagcaaacc
    10561 ggcgatggta gcggaagact acgccaaccg cctgcgtaag aacctcaaaa aattcgagaa
    10621 gtgggctcgt caggaaggga ttgaatgtta ccgcctgtat gacgccgatc tgccggaata
    10681 taacgttgcc gttgaccgtt atgccgactg ggtggtggtg caggagtatg cgccgccaaa
    10741 aactattgat gcgcacaaag cgcgtcagcg tctgttcgat attatcgctg caaccatttc
    10801 ggtactgggg attgcgccaa acaaactggt gctgaaaacc cgtgaacgtc agaagggcaa
    10861 aaatcagtac cagaaactgg gcgagaaggg cgagtttctg gaagttaccg aatataacgc
    10921 tcacttgtgg gtgaacctga cggattatct cgatactggt ctgttcctcg atcaccgtat
    10981 cgcccgtcgt atgctcggcc agatgagcaa aggcaaagat ttcctcaacc tgttctctta
    11041 taccggcagc gccaccgtgc atgcgggatt aggcggtgca cgcagcacca ccaccgtgga
    11101 tatgtcgcgt acttatctgg agtgggcaga acgcaacctg cgtctgaatg gcctaaccgg
    11161 gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg ctgcgtgagg caaatgaaca
    11221 gttcgatctg atctttatcg atccgccaac cttctctaac tcaaaacgaa tggaagatgc
    11281 gtttgatgtt cagcgcgatc atctggcgct gatgaaagat ttgaaacgtc tgctgcgtgc
    11341 aggtgggacg atcatgttct cgaacaacaa acgtggcttc cgtatggatc tcgacggcct
    11401 ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa acgctctccc aggatttcgc
    11461 ccgtaaccgc cagatccaca actgctggct gattaccgca gcctgaaagg aatagtaatg
    11521 tcattaatca gtatgcatgg cgcatggctg tcgttcagcg acgcgccgct tctcgataac
    11581 gcagaactgc atatcgaaga taacgaacgt gtttgtctgg tgggccgcaa cggcgcaggc
    11641 aaatcgacgt taatgaaaat cctcaaccgt gaacaagggc tggatgacgg tcgcattatt
    11701 tacgagcagg atttgattgt agcgcgtctg caacaggatc cgccgcgtaa cgttgagggt
    11761 agtgtttatg atttcgttgc cgaaggcatt gaagaacaag cggaatacct gaaacgctat
    11821 cacgatattt cgcgcctggt gatgaacgac ccgagcgaga aaaatctcaa cgaactggcg
    11881 aaggttcagg aacagctgga tcaccacaac ctgtggcagc tggaaaaccg catcaacgaa
    11941 gtgctggcgc aactggggtt agatcctaac gttgcgctgt cgtcgctttc cggcggctgg
    12001 ttgcgtaaag cggcattagg acgcgcgctg gtgagcaatc cgcgcgtatt gctgctggac
    12061 gaaccgacta accacctgga tatcgaaacc atcgactggc tggaagggtt cctcaaaacc
    12121 ttcaatggga cgattatttt catctcccac gaccgttcgt ttatccgcaa tatggcgacg
    12181 cgcattgttg atctcgatcg cggcaagctg gtgacctatc cgggcaatta cgatcagtac
    12241 ctgctggaaa aagaagaagc cctgcgcgtg gaagaactac aaaatgccga gtttgatcgc
    12301 aaactggcgc aggaagaagt gtggattcgc caggggatca aagcacgccg tacccgtaat
    12361 gaaggccgcg tacgcgccct gaaagcgatg cgtcgcgagc gtggcgaacg tcgcgaagtg
    12421 atgggtaccg caaagatgca ggtggaagag gccagccgct ccggtaagat cgttttcgaa
    12481 atggaagacg tttgctacca ggttgacgga aagcaactgg tgaaagattt ttctgcccag
    12541 gttctacgtg gcgacaaaat tgccctgatt gggccgaatg gctgcggcaa aaccacgctg
    12601 ctgaaactga tgctcggtca gcttcaagcg gacagcgggc gtattcacgt tggcaccaaa
    12661 ctggaagtgg cttatttcga tcagcaccgc gcggaactgg atcccgataa aacggtgatg
    12721 gataaccttg ccgaaggtaa gcaagaggtg atggttaacg gcaagccacg ccacgttttg
    12781 ggctatttgc aggacttcct gttccatccg aaacgggcga tgacgccagt acgtgcgctt
    12841 tctggcggtg agcggaaccg cttgctgctg gcgcgtttgt tcctcaaacc aagcaactta
    12901 ttgattcttg acgaaccgac caacgatctt gatgtcgaaa cgctggaact gctggaagaa
    12961 ctgatcgaca gctaccaggg cacggtattg ctggtaagcc acgatcgtca gtttgtcgat
    13021 aacaccgtta cagaatgctg gatcttcgaa ggcggcggta aaattggtcg ttatgtcggc
    13081 ggttatcatg atgcccgtgg tcagcaagag cagtatgtgg cgctcaaaca gcctgcggtg
    13141 aaaaaaaacg aagaacccgc cgcgccaaaa gcagaaactg taaaacgcag cagtagcaaa
    13201 ctaagctata aattgcagcg cgaactggag cagctaccgc aattgctcga agatctggag
    13261 gcgaagctgg aagccctaca gacgcaggtg gcggatgctt ccttcttcag tcagccgcat
    13321 gagcagacac aaaaagtgct cgccgatatg gctgctgcag agcaggagct ggagcaagcc
    13381 tttgaacgct gggagtatct tgaagcgtta aaaaatggtg gctgatcgca aaatagtcga
    13441 tttaaacaga gacagctgac aacggtaaaa ttgcctgatg aactgggttt atcaggcatg
    13501 aaaacaaaca ctttgttagt catctgtaaa atagcgcatc attaaggagt accaatgtgc
    13561 gaacatcatc atgccgcgaa gcacatcctg tgctcgcagt gtgacatgct ggtggcgtta
    13621 ccgcgccttg agcatggtca gaaagcggca tgtccccggt gtggcacaac gttaaccgtg
    13681 gcgtgggatg ccccccggca gcgtccgacc gcctatgcgt tggctgcact gttcatgctg
    13741 ttgctgtcca acttgtttcc ttttgtgaat atgaacgttg caggagttac cagtgaaatt
    13801 acattactgg aaattcccgg cgtgcttttt tctgaggact acgccagcct cggcaccttt
    13861 ttcctgttgt ttgtgcaact ggttcccgcg ttttgtctga taaccattct gttactggtg
    13921 aatcgcgcgg aattaccggt ccgtttaaaa gagcaactgg cacgggtgct ttttcaactc
    13981 aaaacctggg gaatggcgga gattttcctc gcgggtgtgc tggtcagttt cgttaaactg
    14041 atggcttacg gcagcattgg cgtaggcagc agctttctcc cctggtgttt attttgtgtc
    14101 ctgcaactgc gcgcttttca gtgcgttgat cgtcgctggt tatgggacga tatcgccccg
    14161 atgccagaac tgcgccagcc gctaaaacca ggcgtcacgg ggatacgtca ggggctgcgt
    14221 tcgtgctcct gttgtacggc aatccttcct gctgatgaac ccgtgtgccc gcgctgtggt
    14281 actaaagggt acgttcgacg tagaaacagc ctgcaatgga cactcgcgct gcttgttacg
    14341 tccatcatgc tgtatcttcc ggctaatatt ttgcccatca tggtgacgga tttattaggc
    14401 tcgaagatgc catcgacgat tctcgctggg gtcatcctgt tatggagcga aggctcttat
    14461 cccgtcgctg cggtgatctt tctggccagt attatggtgc caacgttaaa gatgatcgcc
    14521 atcgcgtggc tgtgttggga tgccaaaggg catggcaagc gcgacagtga aagaatgcat
    14581 ttgatttatg aagttgttga gtttgtaggc cgctggtcga tgattgacgt tttcgttatc
    14641 gcggtgctct cggcgctggt gcgtatggga ggtttaatga gtatttatcc ggcaatgggt
    14701 gcattaatgt ttgctttagt cgtcataatg acaatgtttt ctgctatgac gtttgacccg
    14761 cgtttgtcgt gggatcgtca acctgaatca gagcatgagg agtcctgaca gtatggaatc
    14821 taataatggg gaagccaaaa tccagaaagt gaagaactgg tctcccgtgt ggatatttcc
    14881 tatcgtcacg gcgctcattg gggcctgggt tcttttttat cattacagcc atcagggacc
    14941 ggaagtgacc ctgatcaccg cgaatgcgga aggaattgaa ggtggcaaaa ccaccattaa
    15001 aagccgtagc gttgacgtcg gcgtggttga aagcgccaca ctggctgatg atttgacaca
    15061 cgttgaaatc aaagcgcggc tgaattccgg tatggaaaaa ttgctgcata aagataccgt
    15121 cttttgggtg gtgaaaccgc agattggtcg agaagggatt agcggtctgg gaaccctgct
    15181 gtctggggtt tatatcgaac tgcagccagg cgcgaaaggc agcaaaatgg ataaatacga
    15241 tttgctggac tcgccaccgt tggccccgcc tgatgcgaaa ggtatccgtg tggttctcga
    15301 tagcaaaaaa gccgggcagc tctcgccagg agatccggtg ctgttccgtg gctatcgggt
    15361 aggttcggtt gaaaccagca ccttcgatac gcaaaaacgc aatatcagtt atcaactgtt
    15421 catcaatgca ccttatgacc gactggtgac cagcaatgtt cgcttctgga aagatagtgg
    15481 cattgcggtt gatctgacgt cagcgggaat gcgtgtggag atgggctcat tgacaacgct
    15541 gctgagtggc ggtgtcagct ttgatgtgcc ggaaggtctg gatttagggc agccagtggc
    15601 accgaaaaca gctttcgttt tgtatgatga tcagaagagc attcaggatt cgttgtacac
    15661 cgatcacatt gattatctga tgttctttaa agattcggta cgcggtctgc aaccgggagc
    15721 tccggtagaa ttccggggta ttcgcctcgg taccgtaagc aaagtgccat tctttgcgcc
    15781 gaatatgcgt cagacattta acgatgatta ccgtattccg gtgctgattc gtatcgagcc
    15841 agagcggctg aaaatgcagc ttggcgaaaa tgcggatgtt gttgagcacc ttggcgaatt
    15901 gttgaaacgt ggtttacgcg gatcgctgaa aaccggaaac ctggtcactg gcgcactgta
    15961 tgttgatctc gatttctatc caaatacgcc tgcaataacc ggtattcgtg aatttaatgg
    16021 ttatcagatt atcccgaccg ttagcggcgg cctggcgcaa atccagcaac gactgatgga
    16081 agcgttggat aagatcaaca aactgccatt gaatccgatg attgaacagg caaccagtac
    16141 gctttctgaa agtcagcgca caatgaaaaa cctgcaaacg acgctggata gcatgaacaa
    16201 gatcctcgcc agccagtcga tgcagcagtt gccgacggat atgcagtcaa cgttgcgtga
    16261 attgaatcgc agcatgcagg gcttccagcc cggctccgca gcctacaaca agatggtggc
    16321 ggatatgcag cgccttgatc aggtgttgcg agaactgcaa ccggtgctga aaacgctcaa
    16381 tgagaagagt aacgcgctgg tatttgaagc gaaggacaaa aaagatccag agccgaagag
    16441 ggcgaaacaa tgaaaaagtg gctagtgacg attgcagcac tgtggctggc cggatgcagc
    16501 tccggcgaaa ttaataaaaa ctattaccag ttacctgtgg tgcagagcgg tacacaaagt
    16561 accgccagcc agggcaatcg cctgttatgg gtagagcagg tcgctgtacc tgactatctg
    16621 gcggggaatg gtgtggttta tcaaaccagt gatgtgaagt atgtgattgc caacaacaac
    16681 ttgtgggcca gcccgttgga tcaacagttg cgcaacaccc tggtagccaa cctgagcacg
    16741 caactgcccg gctgggtggt tgcctcccag cctctgggaa gcgcccagga cacgctcaat
    16801 gttaccgtaa cggagtttaa cggtcgctat gatggcaagg tcattgtcag tggtgagtgg
    16861 ctgttgaacc accagggaca actgatcaaa cgtccgttcc gtctggaagg agtgcaaact
    16921 caggatggtt acgatgagat ggttaaagtg ctggccggtg tctggagtca ggaagccgct
    16981 tctattgcac aagagataaa gcgtctacct taattataaa gatttgtaaa tataaccgtc
    17041 tccggtatgt tgcctgaggc ggtttttttg tttctaacgt gcggaaaaat ttgttcctct
    17101 tcacattttt tgtacaaccg acatgcccgt gtagctcaca aatatgacag tggcgtgaat
    17161 tttgcgcatt gacggcagtt atgattcgcg gtattgctta actgtgattg cacatttagt
    17221 aatcactgtt ttcttttcca ccagaaacca gtatgaggga aacgaggcat gaagagacaa
    17281 aaacgagatc gcctggaacg ggcacatcaa cgtggttatc aggccggcat cgccggacgc
    17341 tcaaaagaaa tgtgtcccta tcagacgctg aatcaaaggt cacaatggct gggaggctgg
    17401 cgagaagcca tggcggacag ggtagtaatg gcctgattct gtctctttaa aaagaaacct
    17461 ccgcattgcg gaggtttcgc cttttgatac tctgtctgat tataatcaga aggcagacgt
    17521 atcctggaac agaccgactt tcaggtcgct ggcggtatag atcagacgac catcaaccag
    17581 cacttcgcca tccgccaggc ccataatcag acgacggtta acaatgcgtt taaagtgaat
    17641 acggtaggtc acttttttcg ctgtcggcag tacctgacca gtgaatttca cttcgccaac
    17701 gcccagcgcg cggcctttac cttcgccgcc cagccagccg aggtagaacc ctaccagctg
    17761 ccacattgcg tccaggccca ggcatcccgg cataaccgga tcgccaataa agtggcatcc
    17821 gaagaaccac agatccggat tgatatccag ttctgcttca acataccctt tgtcgaagtt
    17881 accacccgtt tcggtcattt tgaccacacg gtccatcatc agcatgttcg gtgctggcaa
    17941 ttgcgggcct ttagcgccaa acagttcacc gcgacctgag gcaagaaggt cttcttttgt
    18001 ataggattcg cgtttatcta ccatgttctc tgtaagcctt attttattga agcacgcagg
    18061 atagctaaca cgtgtacgct gaacaagtcc gatcagttcg gaataaacca gttcagccaa
    18121 cgtaatggcc atggaaaacg gtgacgtcct tcctgttgcg atgcttgcgc gatacgttcc
    18181 tggatggttt gcatcagcgt cgtttggcct tcgccatccc acaccagatt taataataac
    18241 ggcagtgcgt cagtcacatc gtctactgcc cagatggtga atttgccttc ttctaccgct
    18301 ttcaccagtt cactgtgaag acttaaatgg cgaacgttag ctgtggggat aatgacacct
    18361 tgtttcccgg ttaactcacg ttgctggcaa atagcaaaga agccttcgat tttctcattt
    18421 aaaccaccga ccggctgggc gcgaccgaac tgatcgactg aacctgtgat agcgatactc
    18481 tgattcaccg gcacatcggc gagggcgctt atcagggcgc agagttcagc catcgaggca
    18541 ctatctccat caacttcact gtatgactgc tcaaatgtca gcgatgctga gaaggggatc
    18601 tgttgctcaa gctgtagttc cgacatcagg aacgcttgca tgatcatcat ccctttcgca
    18661 tggatattgc cgccaagctc cgctttgcgt tcgatgtcgg tgaattcacc atcgccaata
    18721 tgcacaacgc agctaatgcg agaaggttcg ccaaaagcgc gtggatgacc cggaaattca
    18781 atgaccgaaa gggcgttaat ttgcccgatg cgttcgcctt cggtttcaat caggatttgc
    18841 tcctgaagga tctcatcctg catacgttca gcgaggaaac cttcgcgcca ttcacgctgc
    18901 tgcagcatta agtttagctg ctcgccggag aaggtgtcgc catcacacag ggaggcgacc
    18961 tctttacact ggcggaggat ccactgcggg ctaagcggaa gtgtttcttg ttcaccggtg
    19021 tagcgtgctg cttcgcggat aagtaccggc caggcatccg ctcccggggc aggtaagtga
    19081 ttatgtctgg cggtaaatgt cacccagcga caccactggg ttactgactc cgcatcgaca
    19141 atctgcagag tatcttcaaa ttcgctataa atagcctgct ctgaaagctc tggctccatc
    19201 tcctggaaat cagccaatga ttcgcgttcg cctaccagaa tgactttcag cttcaatggc
    19261 atcgaaggca cagagacggg gagagggcgc gactcatcaa acgcaaccca gtcaaaacgc
    19321 tcgcggttaa cgatattttt cagccgcatc cacagcagag gttgcgccag cagtgtacgc
    19381 aaagagatga tgagaatacc gccatttgcc tgatgcacca gaccaggctg cagggtaatg
    19441 tcgccattaa actggcgcag gcagccaaag agttgctccg cttctaccca gtcggcagcg
    19501 acaacttgcg ttaaagtcgc aaaattatca tctgcactca ctgcgtgacg taagcggatg
    19561 gagtggccgg aaacctcata gtgaccgccc accagttggc ctgcatcgct ttgtagcgta
    19621 cgcgcggcat tggcaataag attcagatac tcagactctt ccggggcctt cgccagcata
    19681 aaagaggagg atgctcgcgt atgcagcaac tgctccagcg caaattgcag ccgcggttga
    19741 gtatcactga ataaaggatc gttttcgtca atcaaatgtg gctgagcaaa tatttcctga
    19801 tagctatcgg tatcaggaac caggtcacgc catgcaagtt tcgtaatggt caaagttgat
    19861 gttttttagt ctgttgtcaa agccgcgatt ataccgtaac cggcactaca gcacacgtag
    19921 aaagcaccga caatactcct ggcatgggcg ttaaagctca caggatggag attcttttct
    19981 tcactggcct aaaaagctga tattctgtaa agagttacac ggtaacattg agatcgctat
    20041 gaaatatcaa caacttgaaa atcttgaaag cggttggaaa tggaagtatc tggtgaagaa
    20101 gcatcgcgaa ggggagttaa tcacccgtta catagaagcc agtgccgccc aggaagccgt
    20161 cgatgtgttg ctctcgctgg aaaatgaacc cgtgctggta aatggctgga ttgacaagca
    20221 tatgaatccg gaactggtca atcgcatgaa gcaaaccatt cgggcaagac gaaagcgcca
    20281 ttttaatgcg gaacaccagc atacgcgcaa aaaatcgatc gatctggaat ttattgtctg
    20341 gcaacgtctg gctggtcttg cgcagcgtcg cggtaaaacg ctttctgaaa cgattgttca
    20401 gctgattgaa gatgcggaaa acaaagagaa atacgcgaat aaaatgtctt ctctgaagca
    20461 ggatctgcag gcattgctgg gtaaggaata actgacgaaa gtcagttcaa tttactaaag
    20521 gcaaaaaaaa ccccgcagca gcggggtttt tctaccagac gagaacttaa gcttgcggct
    20581 gagttacaac gtctttgata cctttaactt cgatctctac gcgacgatcc ggagccaggc
    20641 agtcgatcag tgcagcacgc tgtttcacgt tgtcacaggt gttgccagta accgggttgg
    20701 attcgcccat accacgtgcg gagatcttgt ctgccgggat acctttggag atcaggtaat
    20761 caacaacaga ctgagcacgg cgctcggaca gaccctggtt gtaagcgtca gaaccgatgc
    20821 ggtcggtgta acccagaaca actacggaac cgtctttcgg atccaggttg ctcagctggc
    20881 tgtacagctg atccagagca gcctgacctt ccggtttcag ggttgctttg ttgaagttga
    20941 acagaacgtc agacttcaga gtgaagtgct tggtctgtac ttccggtgcc ggagctggag
    21001 ccggagcaac tactggagct gcttcgccct gaccgaaacg gtaggaaaca cccaggctca
    21061 gcatgccgtt gtccggacga gtgccgatgg tgtgtgcgtc accgatgttg ttggtccact
    21121 ggtattccag acgggtagcg atttcaggag tgatcgcgta ctcaacaccg ccagcgaaga
    21181 ccggagaaac gccggtgtcg tggtttttac cataaacgtt ggatttagtg tctgcacgcc
    21241 ataccatacc acccagacga gtgtagatgt ccaggtcgtc agtgattggg taacccagtt
    21301 tagcggtcag ttgaacgccc tgagctttgt atgcaccgtt ttcaacgctg cctttgtacg
    21361 gcatacgacc taaccagtcg taacccattt caaagccaac atacgggtta acctggtaac
    21421 caccaaaagc accagcgccc agttggtttt catgggtcgg gccattgttg ttgatgaaac
    21481 cagtgtcatg gtactgggac cagcccagtt tagcaccagt gtaccaggtg ttatctttcg
    21541 gagcggcctg cgctacggta gcgaaaccag ccagtgccac tgcaatcgcg atagctgtct
    21601 ttttcatttt ttgcgcctcg ttatcatcca aaatacgcca tgaatatctc caacgagata
    21661 acacggttaa atccttcacc gggggatctg ctcaatatta actctaccga tatcttcggc
    21721 ttatgccgag cacccctggc gatgtaaagt ctacaacgta gttgaaaact tacaagtgtg
    21781 aactccgtca ggcatatgaa aaaaaagtct tgtataaggt atgtttaatc ttttttgtca
    21841 gcgacaattt acagaagaga atcgcggaaa ccgcttcaga caagcctccg caaggaaaat
    21901 tagtcacgac tgaaagcatt ggctgggcga caaaaaaagt tccaggatta atcctaaatt
    21961 tacttaatga tacaaattag agtgaatttt tagcccggaa agttgtctcg tggcgtgaga
    22021 ggatgcgctt accggacgca taataaaccc catagcgtta ccttcatttg ccgcatcaac
    22081 aagttcagca tgctcttctt cagtcaaatc atctgccaac caaccgatca ccacactgta
    22141 attgcccgtg cgtaaagcgc gaaccattga ctccacagtg tggcaagggg agagctggct
    22201 aatctgcatt actttcgtta agggtagccc agatgcctga acccattccc gactcagttt
    22261 ttgttgcggt gttaaccaga gttgccagcg cgattgctga ccgagttgct gtaacaatgg
    22321 caacagtaga agttgcgtca tcatgggctg atcttcgcga tagacaactt cactgataag
    22381 cccggctgta gtgttttccg tagagacacg cgcaatttta cttgctgcgg atgagaacga
    22441 cgaagaacga tgtgcatagc ctgaagtgta cataatcaat ccagcccctg tgagttactg
    22501 tatggatgtg cagtacatcc agtgacaaca aagatcaacc ctattttcgg aaagagcctc
    22561 gcaaattttg tcgttggtga cgggaaaaca taaattaatc ttgccgctta agaataagtt
    22621 gcctattttc gtagttaacg gatccgttaa tgtgaatcat tcttttatgt tatgatttta
    22681 aaaggaattt tatgaaaagc ctctcctata agcggatcta taaatcacaa gaatacctgg
    22741 caacgttggg cacaattgaa taccgatcat tgtttggcag ttacagcctg accgttgacg
    22801 acacggtgtt tgcgatggtt tctgatggtg agttgtatct tcgggcttgt gagcaaagtg
    22861 cacagtactg tgtaaaatat ccgcctgtct ggctgacata taaaaagtgt ggccgatccg
    22921 ttaccctcaa ttactatcgg gttgatgaaa gtctatggcg aaatcaactg aagctggtgc
    22981 gtctgtcgaa atattctctc gatgcagcgc tgaaagagaa aagcacgcgc aatacccggg
    23041 aaagactgaa agatttgccc aatatgtctt ttcatctgga agcgattctc ggggaggtgg
    23101 ggattaagga tgtacgggcg ttacgtatac ttggggcaaa aatgtgttgg ttgcgactgc
    23161 ggcagcaaaa cagtctggtg acagaaaaga ttctgtttat gcttgaaggt gccattatcg
    23221 gcattcatga agctgcgctc ccggtggcac gccgccagga gcttgcagaa tgggctgact
    23281 ctcttacgcc gaaacaggag tttcctgcgg aacttgagta atctggcgtt gcagacgacc
    23341 aatctcaggc agtaatgcaa tcaataatcc aacttgttgt acgaccagag gttctttgct
    23401 gtctgcccgt ggttcaagtt gctgcatccg ctgtttcagg ctagctaatg cctcattgac
    23461 gcgttcttca tcagcaggtt gatgatgtaa cgcgtcatca acatagcaaa ctgcgtcatc
    23521 aagaaacgcc agaatttcag gattagttaa ctgctcccgg tgagcaccga gggctgagat
    23581 atagctggta aacgtatggt taaggcacag caaccgaaac gcggcttcgc gaatttgcgg
    23641 ggtaacgttc ggctcgctgg acatatttga taccaccgac gccagctcag catcacggtt
    23701 gtgtgcatcg cggcgggcaa tacgatacgc cagacggtta tcacgcccct gatggtattg
    23761 ctccagtatg gcatcgagat accgacagtt ggcctctgtg gcgcgttcga gcatgcgcgg
    23821 cagattgcga aactgccagt caggccagat gtagctcact gccgcccacg caatggcaca
    23881 accaatcagc gtatcgatta cgcgaggtaa cgctacttca aaaccttcac ccagtaagtt
    23941 aaaacacagt agcaccaaaa gtgtgatgaa catcgttgca tgagcgtatt gcacgttacg
    24001 gaaggcaaaa aagagcacgc cggtaataac cagcagcacc agctgccctt ccagtgatgg
    24061 cacaaaccac agcacaggaa tgccaatggc gatacctacc agcgtaccaa taatccttaa
    24121 cttcaggcgg tggcgcgtgg cgttatagtt tggctggcag acaaacaaac ttgtcagcaa
    24181 gatccaatac ccgtgatgca ttccggttat ctgaatgatg gcgtagccga agcacaacac
    24241 cagcgacatt cttaccgcat gacggaagag ggcggattcc ggcgtgaagt gacggctaag
    24301 acgcagccag atatcactca acccgtgcgg gctgtcatca gcgagctcat tttcgtcatt
    24361 attatggggt agtgcctggg cctgttctga ttcaattgtt gccagttggg catcaatggc
    24421 gcgtaaattg ttcagcaaaa atcccagtgt tttgagtaaa tcggcgggtg cgccgttatc
    24481 gcgcatccgc tccagcgcag catcaatatg cgtaaaagcg cgctcaaaat gcggatcatg
    24541 ttgataaggc tgacgcaaca aaatacagcg tgacagttgc tggcacgcct ggccctgcat
    24601 cgacatcagc cgctgaaaac ggaacagcac gtcgctgtgg cgaaaatgtt cacgcaatgt
    24661 ttgatactga atatgagaag agctggcacg ctcgtgaata tcctgtgcga caaagtaata
    24721 atgcagcgtg cgacgcgttc cccgttgacc acgatcgcca cgtaagcggg tcagcagcga
    24781 gagtttcgtc tgattcaatg tcgccatcag cagaccgttg gcgagagcca aatcgtacag
    24841 cggtgcctgg ctttcatctt caatatcagg atcaaacatg cgcgacttga gctcaagata
    24901 acgcgccagt tgttcatagc aacgcgccag gttgtcctgc agcgggcgga ccgggaacag
    24961 cagatgacca ataagtgtca ggacgttgta ccagacggca ccggccagca gatacatcgg
    25021 ctgctgatac cagtgctcat acagtgatgt tcccaacata gtgtaaatgg cgatcagcaa
    25081 tgcaccgaag gcaattgttg catagcgttg acccagaccg ccgagcaaaa tgaagccgct
    25141 ggtagagagc gttaagccaa tcgcaaatag ccagggccag ggaaacagca attctactga
    25201 ggccgaggcg ataaaaaagc agaacagcgt aatgatgagg ttacgcaaac gtcccgccag
    25261 tcggtcatcg agatcggtca gcgccgctgc caccatcccc agcgttagcg gaatcgtcag
    25321 ttttacatca cccagccacc acggaaacgc tgtggttcca caaagcgcaa taaaaatacg
    25381 cgcgtaatac agccaggcgc tgttccaggt atagcgtttg agcaaaggac ttagcataaa
    25441 ggccatatta gtggattatt caaaacgacg acgtgcattg gcttcacgcg cagcttgcgc
    25501 tgtttctacc gataccacgc gacgaccaac cggccatagt gcaatagcgg caattttaaa
    25561 gttcgcaatg ccgacaggaa tgccaatgat tgaaatacat tgtgcgatgc ccgttgcaat
    25621 gtgcatcagg cataaccacc agccaaagaa aatcagccag aaaatattca ataccgtacc
    25681 gccagtattc agcagcacat ttttgccagc cgggttcagt tcatcgacat gaatagcttc
    25741 attgccataa ggcaccagag acagtttagt gatctcccag caggatcgtg tcagcggtaa
    25801 ggtaaaaatc agcgcaatac tgaccagagt cgccaacagc cagcccagag tggtggcaaa
    25861 tccgccaagc acaaagttca gaatgttcaa aacggtacgc ataaaacctc gctttactgt
    25921 ggttttcagt aatgggcggc aattgtaacg tttttttggg ctggagcacg ttttctctga
    25981 cggttacact gataagaaat aatttcgtgt ggatctacag agtcatggaa ctgaaagcga
    26041 caacgcttgg aaaacgtctg gcacagcacc cttacgatcg ggcggtgatc ctcaatgccg
    26101 ggattaaagt ctccggcgat cgccacgaat accttattcc tttcaatcaa ttactggcga
    26161 ttcactgtaa gcgcggtctg gtatggggcg agctggaatt tgtactgccg gacgaaaaag
    26221 tggtgcgtct gcacggtacc gaatggggcg agacgcagcg tttttaccat catcttgatg
    26281 ctcactggcg gcggtggagt ggcgagatga gcgaaattgc gtctggtgtt ttacgccagc
    26341 aactggattt gattgccacg cgcactggag aaaataaatg gctgacgcgt gagcaaacct
    26401 ctggcgttca gcaacaaatc cgccaggctt tgtcggcgtt gccgttgccg gttaaccgac
    26461 tggaagaatt cgataactgc cgtgaggcgt ggcgtaaatg tcaggcctgg ttgaaagata
    26521 ttgaaagcgc tcggttgcag cataaccagg cgtataccga agccatgctt accgagtatg
    26581 cggatttttt ccgccaggtc gagtcttcac cgctgaatcc ggcgcaggcc cgggcagtcg
    26641 ttaatggcga gcattctctg ttagtgctgg caggtgcagg aagcggaaaa acgtcggtgc
    26701 tggtggcccg tgcaggctgg ttgctggcgc gtggtgaagc gtcccctgag caaattttat
    26761 tgctggcgtt tggtcgcaaa gccgctgaag agatggacga gcgtattcgc gaacggctac
    26821 ataccgaaga cattaccgca cgcacgtttc atgcgctggc gctgcatatt attcagcagg
    26881 gcagcaaaaa agttccgata gtcagcaaac tggaaaatga taccgctgcc cgtcatgaac
    26941 tctttattgc tgagtggcgc aagcaatgca gcgaaaagaa agcgcaggcg aagggctggc
    27001 ggcaatggct gacggaagaa atgcagtggt cagtgccaga aggtaacttc tgggatgatg
    27061 aaaaattaca gcgtcgtctt gcctcacgcc tcgatcgttg ggtaagtctg atgcggatgc
    27121 acggtggtgc acaggcagaa atgattgcca gtgcacccga agagattcgc gatctgttca
    27181 gtaaacgtat caagttgatg gccccgttat taaaagcctg gaaaggtgcg ctgaaggcag
    27241 aaaacgctgt cgatttttcg ggccttattc atcaggcgat tgtgattctg gagaaaggtc
    27301 gctttatcag cccgtggaag catattctgg ttgatgaatt tcaggatatc tcgccgcagc
    27361 gggcagcgtt gttagcggca ttacgcaagc aaaacagtca gacgacgttg ttcgccgttg
    27421 gtgatgactg gcaggcgatt taccgattca gcggtgcgca aatgtcgctc accaccgctt
    27481 tccatgaaaa ctttggtgaa ggcgaacgct gtgatttaga cacgacttac cgttttaaca
    27541 gtcgtatcgg tgaggtggca aaccggttta ttcagcagaa cccaggccag ctgaaaaagc
    27601 cgctaaacag cttaaccaat ggagacaaaa aagccgtcac gttattggat gagagtcaac
    27661 ttgacgcttt gctggataag ctctctggtt atgccaaacc ggaagagcgc attctgatcc
    27721 tggcgcgtta ccatcacatg aggcctgcca gcctggaaaa agcggcaaca cgctggccga
    27781 agttgcaaat cgactttatg accattcatg ccagcaaagg gcaacaggcg gattacgtcc
    27841 tcatcgttgg cttgcaggag ggaagtgatg gttttccggc tgcggcgcgg gagtcgatta
    27901 tggaagaggc gctactgcca ccggttgagg atttcccgga cgctgaagaa cggcggttaa
    27961 tgtacgtggc gctgacccgg gcacgtcatc gggtatgggc actgtttaac aaagagaatc
    28021 cctctccctt tgtggaaata ctgaaaaatc tggatgtgcc ggtggcgaga aaaccgtaag
    28081 aaacaggtgg cgtttgccac ctgtgcaata ttacttcaga cggtccgcga gataacgctg
    28141 ataatcgggg atcagaatat cgaccgcgtc gttgaaatgc ggcgactgga taatgaagtc
    28201 tgccgttgcc acgttggtgg cgaccggaat gttccatacc gtcgccagac gcagcaaggc
    28261 tttcacgtca ggatcgtgcg gcacggcatt tagtggatcc cagaagaaaa tcaatacatc
    28321 aattttccct tctgagatca atgcgccaac ctgctggtca ccccccattg ggccactcaa
    28381 catcgcgttg acgttcatgc cggtcgcgcg ggaaattaag ttaccggtag tgcctgttgc
    28441 atacagtacg tgttgttcca gtaacggttg atgccgttcc acccagctca tcagcatttg
    28501 tttgcagtga tcgtgtgcca ccagcgcaat atgtttccgc gcaggtaaag tgcgagtcgt
    28561 cagttccata atgtacatcc gtagttaact ttcctacaga ttactgtaag cacttatcgc
    28621 tgcaagataa agaccgaaaa agcctgcgca caggcacaaa aatctcagga agatggttgt
    28681 ttttccgccc attgcaggaa agtatttcgc gtttgtgggt cagccagttt aaaccaatac
    28741 ttcagccgtt gttctgtgag cacctgagac tgcggtggaa tagcatccag ctcggaaaca
    28801 cccgaaagca gcgtactgtc atctgccatc atcgtggcaa attgcggtag tgaagcgcgt
    28861 ttcgcggatt tgttatagcg ttcaacctcg acctcataat caatcgtttt tgccgttgag
    28921 gtaatggcga gaatatccag ctttaccgga atcggcgtcg catcgccatc caacaattca
    28981 aggcgcggcg cggcatcaaa atggttagct tcccgctcat tctccaggcg aggcaggcga
    29041 aaattgacct ggttgatgag ctgggtatta aaactgacca ccagcggtgg ggagatatac
    29101 agccgttctt cactattgga aagatgaatc gtcttctcaa cgcgaaacac taactgatgc
    29161 ggtccattat ccagttcaat gctgtccgca ccgcgcagca gagagctgga aaccttcttg
    29221 ccgtcgagta ccagaagatc gacatcggtt gaaagccgca gcgtggtggc aaaaacggat
    29281 accggcaaac ataatgcaat caaggtggtc acgatgccgg ttttcatagg tagctcctgt
    29341 caaaagaccg cccgaataat aatgtgtcaa aatttttctt taatcatact tactaacata
    29401 tagacatatt tcccgctttt gctctcattc attcgtatta gctgcatggt tggcatgtcg
    29461 gctttgtcgt acactttgtg aaacagtctg gaggaaataa tgaaagaaac cgatattgca
    29521 ggcattttaa cgtccactca caccattgcg ctggtggggg caagcgacaa acccgatcgt
    29581 cccagctatc gcgtgatgaa atatctactc gaccaggggt atcacgtcat tccggtttcg
    29641 ccaaaagttg ccggcaaaac gctgctgggc cagcaggggt acggcacgct ggcagacgtt
    29701 ccggaaaaag tcgatatggt cgatgttttc cgtaattcag aagcggcgtg gggcgtagcg
    29761 caggaagcta tcgccattgg cgcgaaaacc ctctggatgc aactgggggt gattaatgag
    29821 caagcggcag tactggcacg ggatgccggg ttgaatgtgg tgatggatcg ctgcccggct
    29881 attgagatcc ctcgcctggg cctggccaaa taaaaaatcc ccggaaggca aaaaccttcc
    29941 ggggatctgt tcagggatta gttacgcaga cgcggggcct ggagttgttt gcggatggtc
    30001 tgcgccagct catccatgga tggctgttca ggatgttcat cctgcaattc gctacttagc
    30061 tgggcttcag ccaggtaggt atgaaccggt aggccgttat cgtcctccat caccacgtga
    30121 taccacggag cggcgcgaag ctcgtcattc accgccagct catcaggcga cggttcagag
    30181 agcgaataaa ccgggtcgat atccacgacc actccgagat aacctaacag ggaatggcgg
    30241 acctgctggc cgataccgaa tttgctggca atcatagtca cctcccggga aatctttacc
    30301 tttgatataa gggtaaaatt ccacatttca agttacatga cgcgacaggc aaaccctttc
    30361 agatatagcc cttccggata ggtagcgatc accggatgat cggccgcctg acggaactgc
    30421 tctataaatt gtacatcacg accggcatca attgcggcat ccgcgatgat tttctgaaat
    30481 aaatcgctgg tcatcagacc ggaacaggag aaagtcagga gaataccgcc ttcattcagc
    30541 agctgaatcg ccagcatgtt gatatcttta tagccacgac acgcgcccat caactggctt
    30601 ttattctcaa caaacttcgg cgggtccatc acgataacgt caaatttttc accgcgatcg
    30661 cgataggtac gcagcaattt aaagacatca tcacggacaa actcagcctt gctcagatcc
    30721 agtttgttca gctcaacgtt ctgccgtgca atatccagtg cttcctggga ggtatcaacg
    30781 ctgacaacct ggctgcaacc gcccatcagt gccgatacgg cgaaaccacc ggtataggag
    30841 aaacagttca gtacgcgttt attttcaacg tagcggcggg tagccaggcg gctgtctcgc
    30901 tggtccaggt agtagcccgt tttgtgtccg tgctgtatgt ccaccagcag cttcatgcca
    30961 tgttcttcaa tcggcagcag ggcaggcggc aactcaccgg tgacgaggcc ctgggtcagc
    31021 tccatccctt cttttttacg taccgcaaca tcgctgcgat cgtaaatcgc acattccggg
    31081 tacagcgttt gcagggcact aattaatgcc gcgcgctgat attctgcgcc agcactcagc
    31141 agttgcagca ccagaaaatt accgaaacga tcgatagtaa tacccggcag gccatcagat
    31201 tctccggcga ttaaacgata gctgtcgagg ccatcttttt gcgccagcca gtcacgccat
    31261 ttttgtgctt gttgcaaacg gcgggaaaaa aaagcaatgt cgatagactc agacgggtca
    31321 aacgtccaga cgcgcgcccg gatttgcgaa gctggcgaat aagcgccgcg tgctaaccat
    31381 tttccctgat gatcaacaat atcgatggtt tcaccgaggc tggctttacc ttccatacgg
    31441 gcaacggccc cggaaaagac ccacggatgg cgacgaagta atgatttttc gcgccctttg
    31501 gctaacacta aacgtacact cataatttac ttttctgtcg atgcaaagaa atgggcgtca
    31561 ttgtccggag ttttccatgg atttgcaacg cgtccattaa ggataaggag agcagcaatg
    31621 tcgaaagtct gcataattgc ctgggtttac gggcgagttc agggcgtagg atttcgctac
    31681 accacacagt acgaagcgaa aagactgggc ttaaccgggt acgccaaaaa tcttgacgat
    31741 ggcagcgtgg aagtggttgc ctgcggtgaa gaagggcagg tagaaaaatt aatgcagtgg
    31801 ttaaagagtg gcggcccgcg ttctgcgcgt gtagaacggg tgctcagcga gccgcatcat
    31861 ccctcggggg aattaaccga ttttcgtatc cgttaaatac attttaccgg ttttggcagg
    31921 ccagcaattt tggtggcttg cttagccgga cctttcggaa acagtcggta taaatagcgg
    31981 ctgttacctt tttcttcgcc aaatttattc gccatcgctt ttaccagcat acgaatcgcc
    32041 ggagaagtat tgaattccag atagaaatca cgcacaaaac gcaccacttc ccagtgttct
    32101 ggcgacagcg aaatcccttc gttctctgca atcaccactg ccagcggctc actccactgg
    32161 ctgctttctt tgagatagcc ttcggtatcc gtttctatct ctttaccttc gaagatcagc
    32221 ataattatta ctaccttaat cagactgccg gcagtgtaac aaaaaacaaa gccccgcata
    32281 aagcgaggct atgaaagtgt tagcgggtga gattaatcgc ggctggcgaa gcccagaatg
    32341 ctcagcaggc tgacgaagat gttgtacagc gaaacataca ggctaaccgt ggcacgaata
    32401 tagttcgttt caccgccatg aatgatgttg ctggtttcaa acaaaatagc gccagaggag
    32461 atcaaaatga agaccgcgct gatcgccaga tgcagagcag gcagctgcag gaagatatta
    32521 gcaaccatac caatcagcac caccacaata cccgccatca gcataccgcc gaggaacgac
    32581 atatctttgc gggtggtcag cacatatgca gagcagcaga agaacactaa cgccgttccg
    32641 cccagtgcca tagcgattac gtcacccatt ccggcagaca gataggtatt cagaattggt
    32701 ccgaggatat aacccagaaa accggtaaag gcgaatgcgg agataatccc ggtcggctta
    32761 ttcgccattt tataggtcag gaacatcaaa ccatacatac ccaccagcgt cagaatcaga
    32821 cccggagatg gcagcatcag caccgtactg gcagttgcgg taatcgccga aaaggccagc
    32881 gtcaggctca gcagaaaata ggtattacgc agcactttat gggtgctaag cagtgatgta
    32941 cggtcatgtg aagaactaac aatacgatcc attgagtcac tctctatgac agatgtaatt
    33001 aattaagcag cataatgata atgcgtaagg gcacccagta gttttaccca tctttacgca
    33061 tttgatctgg aacaggttta acagcggatt atcaggtcat taagcaaata taacgccctg
    33121 agaatttcgg caggcaaaag aaaaaggggt tagcatttag ctaacccctt atcttatctg
    33181 gcggaagcgc agagattcga actctggaac cctttcgggt cgccggtttt caagaccgaa
    33241 gaaaaacaaa taaaaatcag tgtaatacgt gaaattgctg aaaagttaat caaatttaac
    33301 accaagaaaa atcaaggtat taggaagttg ctcatagcta gtataccaag tctttttgcc
    33361 agaaattttt ctggattgtc tacttgctaa ccacgagcgg caaatccagt gttggtgtga
    33421 tttttgtttt acgatcgtaa atcaacacct gattttctgt tttatgcccg ctgaaaattt
    33481 gtttgtcgcg actgctgcct tcgtaatctg agatcccctt ggcttttatg tcgtgaaaat
    33541 tgcacccgaa cggaacgcca actttttgct cggctgcgcg tttagcctga ttccaccagt
    33601 tattcagcgt tttagctatg actttcccgc ctttggttgt attgatcaca tattcgcatg
    33661 tacaggaaga tacatttcgg gctaactgga tcgctgtacg taatcgcggt gaccattcct
    33721 tgatttgttt ggtgccggtt ttgttttgct caatgtagat ccctttatcc ataatatcct
    33781 gccatttcaa ctcaagcaca tcaccgagtc ttgccgcaca gagataggaa atctccattg
    33841 caatgcgtaa ctgtggaatt gcttccgcat atatcgccgc atactgttca tcggtgatgt
    33901 aaacagtgcg ggctttaaga gagaattttc tgactccttt gcatgggtta ttcttcacat
    33961 acccacgctc atatccccac ccgtatactc gactcaggct tgccagttca tgatttgcct
    34021 gggtcttgct ctcaagccct cgtttatcca tgaaaattct tacctgctca gttttgacat
    34081 tatcagcaag cacttttccg aataccatca gcaacgcctt ctgatgttgt cgataatctt
    34141 tttgggttcg gggggccagt tctgtaaatg caggggagtc cataaacatg tgccacaatt
    34201 tagctacggt cattatgttg tggagttttg ctttttccag ttcataattt tgccagactt
    34261 tagctacgct ggttttccgc actcttccta gccctatact tcttgtactt ccttcgggtt
    34321 tccacacgta actgtaacca ttcgatctaa cccgcggtgg cagtacatta tcttttttat
    34381 tttttcttgg tcttcccatt gttcagcgcc tcaaaatcgg gttcagcaga aaccagttca
    34441 gatgtttttg gcatcgttgt cagaccgtgt ggaatatccc tgcggagaac tatgggttcg
    34501 tttttagggc cgattacaaa tgggatgccg tgcagtctta actggtgttg ctgttttgtg
    34561 tatcgctcgt atttcgtgat ctctttaatc tctgctggag atagagttaa ttcgtacatg
    34621 tggtcacgtg cctttacagc atgaccgccg ccactatagc tggtgggcgg cgatatgggt
    34681 tgaacatcaa aaatcagcct gactcgggag cagtttttgc cagatggctg aaacgtattt
    34741 cgcctggtaa cg
//
LOCUS       DAZFDC010000034        34685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFDC010000034 DAZFDC010000000
VERSION     DAZFDC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.034"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..659
                     /locus_tag="ACHJ48_001804"
                     /pseudo
     CDS             1..659
                     /locus_tag="ACHJ48_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228226.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
     gene            complement(809..2173)
                     /gene="mnmE"
                     /locus_tag="ACHJ48_001805"
     CDS             complement(809..2173)
                     /gene="mnmE"
                     /locus_tag="ACHJ48_001805"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO4707374.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSAKTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2279..3925)
                     /gene="yidC"
                     /locus_tag="ACHJ48_001806"
     CDS             complement(2279..3925)
                     /gene="yidC"
                     /locus_tag="ACHJ48_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO4707375.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3928..4185)
                     /gene="yidD"
                     /locus_tag="ACHJ48_001807"
     CDS             complement(3928..4185)
                     /gene="yidD"
                     /locus_tag="ACHJ48_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO4707376.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFNTREH"
     gene            complement(4149..4508)
                     /gene="rnpA"
                     /locus_tag="ACHJ48_001808"
     CDS             complement(4149..4508)
                     /gene="rnpA"
                     /locus_tag="ACHJ48_001808"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO4707377.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVANLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4525..4665)
                     /gene="rpmH"
                     /locus_tag="ACHJ48_001809"
     CDS             complement(4525..4665)
                     /gene="rpmH"
                     /locus_tag="ACHJ48_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO4707378.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4895..4975)
                     /locus_tag="ACHJ48_001810"
     CDS             complement(4895..4975)
                     /locus_tag="ACHJ48_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707379.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            5272..6675
                     /gene="dnaA"
                     /locus_tag="ACHJ48_001811"
     CDS             5272..6675
                     /gene="dnaA"
                     /locus_tag="ACHJ48_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO4707380.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKSVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6680..7780
                     /gene="dnaN"
                     /locus_tag="ACHJ48_001812"
     CDS             6680..7780
                     /gene="dnaN"
                     /locus_tag="ACHJ48_001812"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO4707381.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7780..8853
                     /gene="recF"
                     /locus_tag="ACHJ48_001813"
     CDS             7780..8853
                     /gene="recF"
                     /locus_tag="ACHJ48_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO4707382.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8882..11296
                     /gene="gyrB"
                     /locus_tag="ACHJ48_001814"
     CDS             8882..11296
                     /gene="gyrB"
                     /locus_tag="ACHJ48_001814"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO4707383.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11320..11418)
                     /locus_tag="ACHJ48_001815"
                     /pseudo
     CDS             complement(11320..11418)
                     /locus_tag="ACHJ48_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11536..11934
                     /gene="yidB"
                     /locus_tag="ACHJ48_001816"
     CDS             11536..11934
                     /gene="yidB"
                     /locus_tag="ACHJ48_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO4707384.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12049..12861
                     /gene="yidA"
                     /locus_tag="ACHJ48_001817"
     CDS             12049..12861
                     /gene="yidA"
                     /locus_tag="ACHJ48_001817"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO4707385.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGNTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12907..13563)
                     /gene="yidX"
                     /locus_tag="ACHJ48_001818"
     CDS             complement(12907..13563)
                     /gene="yidX"
                     /locus_tag="ACHJ48_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO4707386.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVANDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13699..13812
                     /locus_tag="ACHJ48_001819"
                     /pseudo
     CDS             13699..13812
                     /locus_tag="ACHJ48_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13809..14873)
                     /gene="cbrA"
                     /locus_tag="ACHJ48_001820"
     CDS             complement(13809..14873)
                     /gene="cbrA"
                     /locus_tag="ACHJ48_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO4707387.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKINAAYWHATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            14974..16188
                     /gene="yidR"
                     /locus_tag="ACHJ48_001821"
     CDS             14974..16188
                     /gene="yidR"
                     /locus_tag="ACHJ48_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO4707388.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRVSQGAHVGVVTVHPKSEKYVFIHGPENPHETWHYDFHHRRGVIVE
                     GGKMNNLDAMDITVPYTSGALRGGSHVHVFSPNGEMVSFTYNDHVMHQFDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGIVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLGNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16190..16522)
                     /gene="yidQ"
                     /locus_tag="ACHJ48_001822"
     CDS             complement(16190..16522)
                     /gene="yidQ"
                     /locus_tag="ACHJ48_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO4707389.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16762..16834
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16828..17241
                     /gene="ibpA"
                     /locus_tag="ACHJ48_001823"
     CDS             16828..17241
                     /gene="ibpA"
                     /locus_tag="ACHJ48_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO4707390.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17353..17781
                     /gene="ibpB"
                     /locus_tag="ACHJ48_001824"
     CDS             17353..17781
                     /gene="ibpB"
                     /locus_tag="ACHJ48_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO4707391.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17977..19638
                     /gene="yidE"
                     /locus_tag="ACHJ48_001825"
     CDS             17977..19638
                     /gene="yidE"
                     /locus_tag="ACHJ48_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO4707392.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19635..20351)
                     /gene="yidP"
                     /locus_tag="ACHJ48_001826"
     CDS             complement(19635..20351)
                     /gene="yidP"
                     /locus_tag="ACHJ48_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4707393.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPDKS"
     gene            20647..22263
                     /locus_tag="ACHJ48_001827"
     CDS             20647..22263
                     /locus_tag="ACHJ48_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO4707394.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTN
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22263..23585
                     /locus_tag="ACHJ48_001828"
     CDS             22263..23585
                     /locus_tag="ACHJ48_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFO4707395.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGSR
                     QEVIAEACKVILKEKAPDIAFSYTTDTEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMHQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23582..24475)
                     /gene="yidL"
                     /locus_tag="ACHJ48_001829"
     CDS             complement(23582..24475)
                     /gene="yidL"
                     /locus_tag="ACHJ48_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4707396.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPIGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24642..26357
                     /gene="yidK"
                     /locus_tag="ACHJ48_001830"
     CDS             24642..26357
                     /gene="yidK"
                     /locus_tag="ACHJ48_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO4707397.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRMIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFLRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26354..27847
                     /gene="yidJ"
                     /locus_tag="ACHJ48_001831"
     CDS             26354..27847
                     /gene="yidJ"
                     /locus_tag="ACHJ48_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4707398.1"
                     /translation="MKRPNFLFIMTDTQAPNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALKPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(27894..28343)
                     /gene="yidI"
                     /locus_tag="ACHJ48_001832"
     CDS             complement(27894..28343)
                     /gene="yidI"
                     /locus_tag="ACHJ48_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO4707399.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGARVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28452..28799
                     /gene="yidH"
                     /locus_tag="ACHJ48_001833"
     CDS             28452..28799
                     /gene="yidH"
                     /locus_tag="ACHJ48_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO4707400.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28789..29151
                     /gene="yidG"
                     /locus_tag="ACHJ48_001834"
     CDS             28789..29151
                     /gene="yidG"
                     /locus_tag="ACHJ48_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO4707401.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29148..29645
                     /gene="yidF"
                     /locus_tag="ACHJ48_001835"
     CDS             29148..29645
                     /gene="yidF"
                     /locus_tag="ACHJ48_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO4707402.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29653..30837)
                     /gene="emrD"
                     /locus_tag="ACHJ48_001836"
     CDS             complement(29653..30837)
                     /gene="emrD"
                     /locus_tag="ACHJ48_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO4707403.1"
                     /translation="MKRHRNINLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            30919..30993
                     /gene="ysdE"
                     /locus_tag="ACHJ48_001837"
     CDS             30919..30993
                     /gene="ysdE"
                     /locus_tag="ACHJ48_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO4707404.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(31117..31206)
                     /gene="tisB"
                     /locus_tag="ACHJ48_001838"
     CDS             complement(31117..31206)
                     /gene="tisB"
                     /locus_tag="ACHJ48_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO4707405.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31771..31869
                     /gene="ivbL"
                     /locus_tag="ACHJ48_001839"
     CDS             31771..31869
                     /gene="ivbL"
                     /locus_tag="ACHJ48_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO4707406.1"
                     /translation="MATSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            31975..33663
                     /gene="ilvB"
                     /locus_tag="ACHJ48_001840"
     CDS             31975..33663
                     /gene="ilvB"
                     /locus_tag="ACHJ48_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO4707407.1"
                     /translation="MASSGTSSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33667..33957
                     /gene="ilvN"
                     /locus_tag="ACHJ48_001841"
     CDS             33667..33957
                     /gene="ilvN"
                     /locus_tag="ACHJ48_001841"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO4707408.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34220..>34685
                     /locus_tag="ACHJ48_001842"
     CDS             34220..>34685
                     /locus_tag="ACHJ48_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707409.1"
                     /translation="MASPINSGIMMANLCPSTFLDKNRNVAAELDIKNNEKKYSPGSN
                     FAKWMLQEIKRLILNIMSGSRSINTDILDYFHPMPGTENNGNRTWVAATGEDEYIEIK
                     QTGDKSFNITLVGRDKPSRKEIPYSGVAVATIIKSLSEKTSALETHSADTVLR"
BASE COUNT         8399 a         8755 c         9158 g         8373 t
ORIGIN      
        1 agccaatata aaagccaaaa tgtttaaaaa tgaattaaat aaagaagata tattgaaact
       61 gatggcagca agagaaaaag tggcgggagc atcagacaaa tggacgaagg catcaggctt
      121 atattctgcg atagtgaaag ggcatacgaa gattgttgct gcctggatgg agacagctga
      181 agtgatagcc agccattatg aaaatgataa agatgtagtg agagaactcc tgtcgctgag
      241 cagaaataat gcagtttgct ctttgtatgt tgccagctat aagacaatga gtaagcaggt
      301 cattgatgta tatctgaatg cggcgattcg cctggcgttg caacacgggt tcactttcga
      361 tgagattttg gagcagttta cccgtgactt tgatgggaag tcattctctc ttgcggtaga
      421 gaaagcggat gatatatatg ggtctctggc tgaaaatatt caaaattgtg gttggtgaaa
      481 atgaaaatta tcttaaagat gtgatgatgc aactggaagc gaagaataat gaaggcaaat
      541 atgtaataag caaggcaaat ggcaatcctg tatttaaaga actattctgg aaagcaatag
      601 acgaatttaa ctttccgcaa gaggagctta atcggctaaa gcaatatcgt tctctgtaaa
      661 gacaggactg caagatgttg aaagtcgctc cagccagtag taaaggcagt tttggtgcag
      721 gatgctcata aaaaaacaag cgctgttccg ttctgatagt tagtttcccc cagaacggaa
      781 cagtgccctt tatatcaatt tagacgactt acttaccaat acagaagctg gagaaaatcc
      841 gccccagcaa atcatcagag gtaaattccc cggtgatttc gcttaagttc tgctgtgcca
      901 ggcgcaactc ttccgccagc agttcgcccg cccaggcacc caacagttgc gctttacctt
      961 gctgcaaatg ctccgctgcc tgttccagcg cctgaaggtg gcgacgacgc gccaggaagc
     1021 cgccttccat gttggtgtca aagcccatgc tctgtttgag atggttacgc agcacgtcca
     1081 cgccttcgcc agtctttgcc gagagacgaa tcaacgcatg accgttcact tcactcattc
     1141 ccagcgtttc gccagtgata tcggctttat tgcgtaccac ggtgatcggc agtttcgcgg
     1201 gcagacgggc gataaattcc ggccagatct ccgccggatc aacggcgtct gtcgtggtgc
     1261 catcgaccat aaacagcacg cggtcggctt gttcaatttc ttgccacgcg cgctcgatac
     1321 caatacgttc cacttcatcg ctggcttcac gtagcccggc ggtgtcgatg atatgcagcg
     1381 gcattccgtc aatgtggata tgctcacgca acacgtcacg cgtggttccg gcgatatcgg
     1441 ttacgattgc tgcttcacgg ccagccagcg cgtttaacag gctcgattta ccggcattag
     1501 gacgtccggc aatcaccacc ttcatccctt cgcgcaacaa actaccctga cgtgcttcag
     1561 cacgcactgc atcgagatcg gcaatcacgt cattaagctg ggcttcaatt tttccgtcgg
     1621 agaggaaatc gatctcttca tcggggaaat caattgccgc ttcgacgtaa atgcgcaagt
     1681 gggtgagggc ttccaccaga tggttgaccc gtgcggagaa tgcgccttgc agcgagttaa
     1741 gtgccgaacg ggctgcctgt tccgaactgg catcgataag atcggcaatc gcctcggcct
     1801 gggctaagtc gagtttatcg ttaagaaacg cgcgttcgga aaactcacca gggcgagcaa
     1861 tccgcaggcc gggaatggtc agaatgcgtt ttaacagcag gtcgagaatc accggaccgc
     1921 catgaccctg cagttccagc acatcttcgc cggtgaacga gttcgggcca gggaaccata
     1981 gcgcaatccc ctgatcgagc acgctgccat cggcgtcttt aaacggaaga taatcggcgt
     2041 agcgcggctt aggcagttta cccagcacgg tttcggcaac ttcacgggct ttgaagccgg
     2101 agatgcgcag gatgccaacg ccgccacgtc ccggaggcgt ggcctgggct acgatagtgt
     2161 cattatcgct catgatgttc ctgttgcttt gtgtggcgga tacgcggtgc ttatccgccc
     2221 tacgaaaaga aaaaaggcgg tcaactgacc gcccttattt tagcgaaaac tcaccgaatc
     2281 aggatttttt cttctcgcgg ctatgcaggc cacgtttttc cagaccacgg taaatcagct
     2341 gctgctgaat aatggttacc aggttgctga cgatatagta cagcaccaga cctgacggga
     2401 accacaggaa gaacacggtg aagatgaccg gcataaaggt catgatcttc tgctgcatcg
     2461 ggtcggtcac agtggtcggc gacatcttct gaatgaagaa catcgttacg cccatcagga
     2521 tcggcaggat gtagtacggg tcctgtgcag acaggtcgtg gatccacagt gcaaacggtg
     2581 cctgacgcag ttcaacggaa cccatcagca tgtagtacaa cgccaggaag attggcatct
     2641 ggatcagcag cgggaagcag ccgcccagcg ggttaacctt ctcagctttg tacagcgcca
     2701 tcatttcctg gctgatacgc tgtttgtcat cgcccagacg ctcacgcatt gcctgaatct
     2761 tcggctgcag catacgcatc ttcgccatgg aggtgtactg cgctttggtc agcgggtaca
     2821 tgatgccacg aacgataaag gtgatgatga taatggagaa gccccagtta cccacaaagc
     2881 tatggatcca tttcagcagt ttgaacagcg gctgagagat gaaccacaac caaccgtaat
     2941 caacggtcag atccaggtgc ggagcaacag ctgccatttt gtcctggatt tccgggccaa
     3001 cccacagggt gctgttcatc gcgccagtct gaccaggctg aaccagtacc ggctgagatt
     3061 tatagccgat agcggcgatg ccgttaccca gattagcagt atagaagttg ttggtaccgt
     3121 cgttatgcgg gatccacgcc gtcgcgaaat actgttgcag cattgccacc caaccacctt
     3181 tcgaagagat gttcaggttt tcgttatcgg caatggtatc gaacttgtat ttctcgtact
     3241 tctcgtcagg cgtggagtac gccgcgccac ggaaggtgtg cagtgcgaag ttgctgcttc
     3301 cggtatcgag atgcggtggc agagtgatgg attgcttcaa ctgaccaaag gtggagattt
     3361 ccagcggttt ctcgccagcg ttctgcacgt tgtagttgac gttgacagcg taatcacccc
     3421 gtttcaggac aaacgttttg gtaaacgtgt tgcctgccgc gtcggtatac gtcatcggca
     3481 cctgcagttc gttttgacct tcagccagca cataagcgtc tttttcaacg ttatacagcg
     3541 gacgcgggcc gttagccggg ttatctgggc catcacgacc ggtcagaccg ctctgtgcct
     3601 gataaataaa ctgcggtgaa gtttccagca gctggaacgg ctgggtagag ttcagctctt
     3661 tcgggtaagc aggcagcaga gcttgctcaa catcaccacc acgggtgttg atggtcagat
     3721 caagcacgtc ggtcttaacc gagatcagtt tcccctggcc actggccggt acgccctggt
     3781 cggcggcgct acccgctgcg gtggtcgttg tctgcgtggt ctgttgggcc tgaggttgcg
     3841 ggtttttatc ctgctcccag gcttgccaga tcatgaaaga cacgaacagc aaagcgatga
     3901 ctaaaagatt gcgttgcgaa tccatcgtta gtgttctctg gtattaaatg gtccgggcgg
     3961 gacgggatcg tcaccaccag ggtgtaaagg gtggcatttt aatacgcgtt tcaccgtcaa
     4021 ccaactgcct tttatcactc caaacctgcg caatgcctca attccgtagc ttgaacaggt
     4081 tggagtgaaa cgacaatgcg gcccgagtag cggactaatc aggcgttgat agacccgaat
     4141 gagggctatc aggacccgcg agccaggcga cagtggcggc gccataattt ttccaacgct
     4201 tccgagagag cacggttatc gaggttggca accccttttt tcgccaccac cacgaaatcc
     4261 atagccggga gttcatgttg gcgcagacgg aagctttcac gcgtcagacg tttaatccga
     4321 ttgcgttcat gggcgcgtcg aacgtttttc ttggcgactg taagaccgat acggggatgc
     4381 cccagcgaat tcaggcggcc gagaatggta atttgcggcg tgccagcccg ttgtggctgc
     4441 tggaagacga atgtgaattg actgggagtt aacaagcgta actccctggg aaatgcgagc
     4501 ttaaccactc aggggttagc tttattactt agaaacggtc agacgagcgc ggcctttagc
     4561 acgacgacgt gccagaacct gacgaccatt tttagtagcc atacgagcac ggaagccgtg
     4621 agaacggttg cgcttcagta cagacggttg aaaagtgcgt ttcatggcga tttctaccta
     4681 aacttgaata aattcaatgg ctttattgga tatccgccga aaaatgaaac gatggacacc
     4741 gaagccatgg gtgattaaag aggccggatt gtaataattg tacactccgg agtcaattct
     4801 ctttccttat ttaccgcgct tttccgcacc ttttcgcagg gaaaacgtac ggcctcacac
     4861 cagtgaaaac cagcatggcg cgccgggtgg aggattatac gggctgatgg gtaaagcgca
     4921 aggatcgtcc tggatcttta ttagatcgat taagccaatt tttgtctatg gtcattaaat
     4981 tttccaatat gcggcgtaaa tcgtgcccgc ctcgcggcag gatcgtttac acttagcgag
     5041 ttctggatca tcctgtggat aaatcgggaa aatctgtgag aaacagaaga tctcttgcgc
     5101 agtttaggct atgatccgcg gtcccgatcg ttttgcagga tcttgatcgg gcatataacc
     5161 gcagacagcg gttcgtgcgt caccctcaag cagggtcttt tcgacgtacg tcaacaatca
     5221 tgaatgtttc agccttagtc attatcgact tttgttcgag tggagtccgc cgtgtcactt
     5281 tcgctttggc agcagtgtct tgcccgattg caggatgagt taccagccac agaattcagt
     5341 atgtggatac gcccattgca ggcggaactg agcgataaca cgctggccct gtacgcgcca
     5401 aaccgttttg tcctcgattg ggtacgggac aagtacctta ataatatcaa tggactgcta
     5461 accagtttct gcggagcgga tgccccacag ctgcgttttg aagtcggcac caaatcggtg
     5521 acgcaaacgc cacaagcggc agtgacgagc aacgtcgcgg cccctgcaca ggtggcgcaa
     5581 acgcagccgc aacgtgctgc gccttctacg cgctcaggtt gggataacgt cccggccccg
     5641 gcagaaccga cctatcgttc taacgtaaac gtcaaacaca cgtttgataa cttcgttgaa
     5701 ggtaaatcta accaactggc gcgcgcggcg gctcgccagg tggcagataa ccctggcggt
     5761 gcctataacc cgttgttcct ttatggcggc acgggtctgg gtaaaactca cctgctgcat
     5821 gcggtgggta acggcattat ggcgcgcaag ccgaatgcca aagtggttta tatgcactcc
     5881 gagcgctttg ttcaggacat ggttaaagcc ctgcaaaaca acgcgatcga agagtttaaa
     5941 cgctactacc gttccgtaga tgcactgctg atcgacgata ttcagttttt tgctaataaa
     6001 gaacgatctc aggaagagtt tttccacacc ttcaacgccc tgctggaagg taatcaacag
     6061 atcattctca cctcggatcg ctatccgaaa gagatcaacg gcgttgagga tcgtttgaaa
     6121 tcccgcttcg gctgggggct gactgtggcg atcgaaccgc cagagttaga aacccgcgtg
     6181 gcgatcctga tgaaaaaggc cgacgaaaat gacattcgtt tgccaggcga agtggcgttc
     6241 tttatcgcca agcgtctacg atctaacgta cgtgagctgg aaggcgcgct gaaccgcgtc
     6301 attgccaacg ccaactttac cggacgggcg atcactatcg acttcgtgcg tgaggcgctg
     6361 cgcgacttgc tggcattgca ggaaaaactg gtcaccatcg acaatattca gaagacggtg
     6421 gcggagtact acaagatcaa agtcgcggat ctcctttcca agcgtcgatc ccgctcggtg
     6481 gctcgtccgc gtcagatggc gatggcgctg gcgaaagagc tgactaacca cagtctgcca
     6541 gagattggcg atgcgtttgg tggtcgtgac cacactacgg tgcttcatgc ctgccggaag
     6601 atcgagcagc tgcgtgaaga gagccacgat atcaaagaag atttttcaaa tttaatcaga
     6661 acattgtcat cgtaaaccta tgaaatttac cgtagaacgt gagcatttat taaaaccgct
     6721 acaacaggtg agcggtccgt taggtggtcg tcctacgcta ccgattctcg gtaatctgct
     6781 gttacaggtt gctgacggta cgttgtcgct gaccggtact gatctcgaga tggaaatggt
     6841 ggcacgcgtt gcgctggttc agccacacga gccaggagcg acgaccgttc cggcgcgcaa
     6901 attctttgat atctgccgtg gtctgcctga aggcgcggaa attgccgtgc agctggaagg
     6961 tgagcggatg ctggtgcgct ccgggcgtag ccgtttttcg ctgtctaccc tgccagcggc
     7021 ggatttcccg aacctcgatg actggcagag tgaagtcgaa tttaccctgc cgcaggcgac
     7081 gatgaagcgt ctgattgaag cgacccagtt ttctatggcg catcaggacg ttcgctatta
     7141 cttaaacggt atgctgtttg aaaccgaagg tgaagaactg cgcaccgtag cgaccgacgg
     7201 ccaccgtctg gcggtctgtt caatgccaat tggtcaatct ttgccaagcc attcggtgat
     7261 cgtgccgcgt aaaggcgtga ttgaactgat gcgtatgctc gacggcggcg ataatccgct
     7321 gcgtgtgcag attggcagca acaatattcg cgcccacgtt ggcgacttta tcttcacctc
     7381 taaactggtg gatggtcgct tcccggatta ccgccgcgtt ctgccgaaga atccggacaa
     7441 acatctggaa gcaggctgcg atctcctcaa gcaggcgttt gctcgcgcgg caattctctc
     7501 taacgagaaa ttccgcggtg tgcgcctgta tgtcagcgaa aaccagctga aaatcaccgc
     7561 caacaacccg gaacaggaag aagcggaaga gatcctcgac gttacctata gcggtgcgga
     7621 gatggaaatc ggcttcaacg tcagctatgt gctggatgtt ctgaacgcgc tgaaatgcga
     7681 aaacgtccgc atgatgctga ccgattcggt ttccagcgtg cagattgaag atgcagccag
     7741 ccagagcgcg gcttatgttg tcatgccaat gagactgtaa tgtccctcac ccgcttgttg
     7801 atccgcgatt tccgcaacat tgaaaccgcg gatctcgctt tatctcccgg ctttaacttt
     7861 ctggtaggtg ccaacggcag tggcaaaacc agcgtgctgg aagccatcta tacgctcggc
     7921 catggtcggg cgtttcgcag tttgcagatt ggtcgcgtca ttcgccacga gcaggaggcg
     7981 tttgttctcc acgggcgatt acagggcgaa gagcgcgaga cggcgattgg cttaaccaaa
     8041 gacaaacagg gcgacagcaa agtccgcatc gacggtactg acgggcataa agtcgcggaa
     8101 ctggcgcacc tgatgccaat gcagctgata acgccagaag ggtttacttt actcaacggc
     8161 ggccccaaat acagaagagc attcctcgac tggggatgct ttcacaacga acccggattt
     8221 ttcaccgcct ggagcaatct caagcgattg ctcaagcagc gcaatgcggc gctgcgccag
     8281 gtgacacgtt acgaacagct acgcccgtgg gataaagaac tgatcccgct ggcggagcaa
     8341 atcagtacct ggcgcgcgga gtatagcgcc ggtatcgcgg ccgatatggc tgatacctgt
     8401 aagcaatttc tccctgagtt ttctctgact ttctctttcc agcgcggctg ggagaaagag
     8461 acagaatatg ctgaggtgct ggaacgtaat tttgaacgcg atcgccagct aacctacacc
     8521 gcgcatggcc cgcataaagc ggacttacgc attcgcgccg acggtgcgcc ggtggaagat
     8581 accttatcgc gtgggcagct taagctgttg atgtgcgcct tacgtctggc gcaaggagag
     8641 ttcctcaccc gtgaaagcgg gcggcggtgt ctctacctga tagatgattt tgcctctgag
     8701 cttgatgatg agcgtcgcgg gctgcttgcc agccgcttaa aagctacgca atcgcaggtc
     8761 tttgtcagcg cgatcagtgc tgaacacgtt atagacatgt cggacgaaaa ttcgaagatg
     8821 tttaccgtgg aaaagggtaa aataacggat taacccaagt ataaatgagc gagaaacgtt
     8881 gatgtcgaat tcttatgact cctccagtat caaagtcctg aaagggctgg atgcggtgcg
     8941 taagcgcccg ggtatgtata tcggcgacac ggatgacggc accggtctgc accacatggt
     9001 attcgaggtg gtagataacg ctatcgacga agcgctcgcg ggtcactgta aagaaattat
     9061 cgtcaccatt cacgccgata actctgtctc tgtacaggat gacgggcgcg gcattccgac
     9121 cggtattcac ccggaagagg gcgtatcggc ggcggaagtg atcatgaccg ttctgcacgc
     9181 aggcggtaaa tttgacgata actcctataa agtgtccggc ggtctgcacg gcgttggtgt
     9241 ttcggtagta aacgccctgt cgcaaaaact ggagctggtt attcagcgcg agggtaaaat
     9301 tcaccgtcag atctacgaac acggtgtacc gcaggcaccg ctggcggtta ccggcgagac
     9361 tgaaaaaacc ggcaccatgg tgcgtttctg gcccagcctc gaaaccttca ccaatgtgac
     9421 cgagttcgaa tatgaaattc tggcgaaacg tctgcgtgag ttgtcgttcc tcaactccgg
     9481 cgtttccatt cgtctgcgcg acaagcgtga cggcaaagaa gaccacttcc actatgaagg
     9541 cggcatcaaa gcgttcgttg aatatctgaa caagaacaaa acgccgatcc acccaaatat
     9601 cttctacttc tccaccgaaa aagacggtat cggcgttgaa gtggcgctgc agtggaacga
     9661 tggcttccag gaaaacatct actgctttac caacaacatt ccgcagcgtg acggcggtac
     9721 tcacctggca ggcttccgtg cggcgatgac ccgtaccctg aatgcctaca tggacaaaga
     9781 aggctacagc aaaaaagcca aagtcagcgc caccggtgac gatgcgcgtg aaggcctgat
     9841 tgcggtcgtt tccgtaaaag tgccggaccc gaaattctcc tcccagacca aagacaaact
     9901 ggtttcttct gaggtgaaat cggcggttga acagcagatg aacgaactgt tggcggaata
     9961 cctgctggaa aacccaactg acgcgaaaat cgtggttggc aaaattatcg atgctgcccg
    10021 tgcccgtgaa gcggcgcgtc gcgcgcgtga aatgacccgc cgtaaaggtg cgctcgactt
    10081 agcgggcctg ccgggcaaac tggcagactg ccaggaacgc gatccggcgc tttccgaact
    10141 gtaccttgtg gaaggggact ccgcgggcgg ctctgcgaag caggggcgta accgcaagaa
    10201 ccaggcgatt ctgccgctga agggtaaaat cctcaacgtc gagaaagcgc gcttcgataa
    10261 gatgctctct tctcaggaag tggcgacgct tatcaccgcg cttggttgtg gtatcggtcg
    10321 tgacgagtac aacccggaca aactgcgtta tcacagcatc atcatcatga ccgatgcgga
    10381 cgtcgacggc tcgcacattc gtacgctgct gttgaccttc ttctatcgtc agatgccgga
    10441 aattgttgaa cgcggtcacg tctacatcgc tcagccgccg ctgtacaaag tgaagaaagg
    10501 caagcaggaa cagtacatta aagacgacga agcgatggat cagtaccaga tctctatcgc
    10561 gctggacggc gcaacgctgc acaccaacgc cagcgcaccg gcattggctg gcgaagcgtt
    10621 agagaaactg gtgtctgagt acaacgcgac gcagaaaatg atcaaccgca tggagcgtcg
    10681 ttatccgaaa gcaatgctga aagagcttat ctatcagccg acgctgacgg aagccgacct
    10741 ctctgatgag cagaccgtta cccggtgggt gaacgcgctg gtcagcgaac tgaacgacaa
    10801 agaacagcac ggcagccagt ggaagtttga tgtccacacc aatgccgaac aaaacctgtt
    10861 cgagccgatt gttcgcgtgc gtacccacgg tgtggatact gactatccgc tggatcacga
    10921 gtttatcact ggtggcgaat atcgtcgtat ctgcacgctg ggtgagaaac tgcgtggctt
    10981 gctggaagaa gatgcgttta tcgaacgtgg cgagcgtcgt cagccggtag ccagcttcga
    11041 gcaggcgctg gactggctgg tgaaagagtc ccgtcgcggc ctctccatcc agcgttataa
    11101 aggtctgggc gagatgaacc cggaacagct gtgggaaacc accatggacc cggaaagccg
    11161 tcgtatgctg cgcgttaccg ttaaggatgc gattgccgct gaccagttgt tcaccacgct
    11221 gatgggcgac gccgttgaac cgcgccgtgc gtttattgaa gagaacgccc tgaaagcggc
    11281 gaatattgat atttaatggc gttaacaatg cgagcgtgcc tgatgcgcta cgcttatcag
    11341 gcctacgaga ctcctgcaat atattgaatt tgcatgtttt tgtaggccgg ataaggcgtt
    11401 cacgccgcat ccggcaagaa gctataagaa aagggcggag atcatctccg ccctttttat
    11461 ttctgcaatc cgcaattatc atcccgcagt tctatacttt ttgggtcaac acgtaacgct
    11521 ttacaggtga ttatcatggg gctttttgat gaagttgtcg gcgcctttct gaaaggcgat
    11581 gcggggaaat atcaggcgat tttaagttgg gttgaggagc agggtggcat tcaggtgctg
    11641 ctggaaaaac tgcaaagtgg cggcttaggg gccattctct caacctggct gagtaatcaa
    11701 cagggcaatc aaccagttag cggcgagcaa ctggaatcag cactcggcac aaacgcggta
    11761 tccgatcttg gacaaaaact tggcgtggat accagtacgg cttcctgttt actggcagaa
    11821 caattgccga agattattga tgcgctctca ccgcaaggtg aagtgtcacc acaagccaat
    11881 aacgatctgc tttccgcagg catggaactg ctgaaaggga aactcttccg ctaagcaaat
    11941 ggggagcatg caacccgcgt tgcatccccc ttttccctcg ggtgttgtac tgatttttga
    12001 gcggaatcgc gttagcatgg gtcaggaacc aatctacctg gggaactcat ggctatcaaa
    12061 ctcattgcta tcgatatgga tggcaccctt ctgctgcccg atcacaccat ttcacctgcc
    12121 gttaaaaatg cgattgccgc agctcgcgcc cgtggcgtga atgtcgtgct aacgacgggt
    12181 cgcccttatg caggtgttca caattacctg aaagagctgc atatggaaca gccgggcgac
    12241 tactgcatta cttataacgg cgcgctggta cagaaggccg ctgatggtaa caccgtggcg
    12301 caaactgctc tcagctatga cgactatcgt ttcctggaaa aactctctcg cgaagtcggt
    12361 tctcatttcc acgccctgga ccgcaccacg ctgtacaccg ccaaccgtga tatcagctac
    12421 tacacggtgc atgaatcctt cgttgccacc attccgctgg tgttctgcga agcggagaaa
    12481 atggacccca atacccagtt cctgaaagtg atgatgattg atgaacccgc catcctcgac
    12541 caggctatcg cgcgtattcc gcaggaagtg aaagagaaat ataccgtgct gaaaagtgcg
    12601 ccgtacttcc tcgaaatcct tgataaacgc gttaacaaag gtacgggtgt gaaatcactg
    12661 gccgacgtgt taggtattaa accggaagaa atcatggcga ttggcgatca ggaaaacgac
    12721 atcgcaatga ttgaatatgc aggcgtcggt gtggcaatgg ataatgctat tccttcggtg
    12781 aaagaagtgg cgaactttgt caccaaatcc aaccttgaag atggcgtggc gtttgctatt
    12841 gagaagtatg tgctgaatta atctgtgggc gggcaaacag cataaacgcg tttgcccgct
    12901 tactgattat tggtttactg catatacaac cagaaattgc agcggcgcag taatgatgtc
    12961 cagcgtaaca gtcactggca gtaatgctgc ggaaacacca tccggaatgc ctctgggtga
    13021 atagtattca taaaatcgca ctttaaatgg atggtagaac gccatcacct ttgagtaatc
    13081 catgttttta tttttctcat ggattgtgcc ttttaggttt tccagtgacc tggtacagtg
    13141 ttgaacacca caggcaaaac cataatgcgt tgccagtgcc ttttcttctt cgttattcca
    13201 gtagtagtaa agcgatattg tgccggtaaa tttctttttc cccgcattta aaacaaatct
    13261 tgcgtcattg gcaacctgaa tattgtgccg gttaagtgct ggatcattga gcatcttaac
    13321 gatgtcatcg gcaccatcag tgataaggta atccagcgat tcccctacaa aaacatagcc
    13381 ttttgttcca ttactatctt ttgcctgcga tagcccagtg atggtgtctg actgcgattg
    13441 atactttcct gaactaccct ttgcggtatg ggaaaccaga gcatacgaga tacagcctga
    13501 aagcatcagc gccagtggga ataaactggc agccttaaaa aatcccttaa aattcaactt
    13561 catgattaat cctttaaacg agtagctaag ccgttgaaag catactcttc tttccctcct
    13621 gtttagcgcc tgatctccct tttcagattt ctgcccgata taagaccttt tcttatgagg
    13681 tgtttttttg tggactaata tctgttgcaa ggctatgggt tcgcacctgc actggtttat
    13741 atctccgccg tcgcgttgat tggcgcgctc tcttacatcc tgctggtggg cgatgtgacg
    13801 cgcgttggct aatctttcaa ctgtggaata tgcgccatac cactgcgcat aatccacttt
    13861 cttaaagccg gtgcggtcag gcatcggctt tttactatct tgccgaagag ttttaaacgc
    13921 agtttgcggg tggcgtgcca gtaggctgcg ttgatcttct ctggctgctt cagtaacacc
    13981 gaacgcagaa tctctgcgct atccagcgca tagcttatcc cctccagcga gctggcgctg
    14041 ataaatcccg ccgcttcacc aatcagaaag gcgttgtcct taccgcagac aaaatcctgc
    14101 cagcgtgagg gaaatagcac cgtgcatttt tcgcttttta ccgccttacc aaactggaat
    14161 tgaaaggcgc tcattttctc tttcagcgtc gtgaaacgcg tctgaccgtc tttcattgga
    14221 taggcaccgc caaagataaa ataaccgtct ttgctgatac tccatgagta acagtcagtt
    14281 atcgcattat caaagatgca tgagtagaac ggcaccggat gtttctctgc gaaccactgc
    14341 tggatagcga catatttacg aatctgatgg tccgggtaga gatatcgccg caccatcgag
    14401 ttggcaccat ctgcaccgac cagatagcgg gcagtaatat gctgctccca accgtctgca
    14461 cgaaaaatga catgccattt atcatcctca cgccagattt tccggcacag gctatcgtga
    14521 taaacctcaa cgctggcggg gatcagcgat ttcatccaca agtcgaaagc atggcgatta
    14581 atattgatat agcttcgctg gtagttacgc gtgagcgatg cggcgacgtc gacagttttg
    14641 acgctgaaaa tctgtggatt ggcgatcaca tcgacaggaa gcgtcagtcc atcgcgaata
    14701 aaagatcgct gcgcgtccgg tgccagcaga ccgccacaag gcttgctgaa accttcagta
    14761 ccatgctggt gctttttatc cagcgcgatc acctgcattt tgccggctaa ctttcgtgcc
    14821 aacgccgacc ctgccgggcc gaggccaata atcgccacgt cgaaatgttc catcttttca
    14881 ctcacattca tcacgatggc aaaagactat tgtctgaaag tgaagtcctt gtgaagtatg
    14941 atggcgggaa ccgttttgta tcaggaccgc gccatgaaac agataacctt tgctccccgt
    15001 aatcacctgc tcaccaatac caatacctgg acgcccgaca gccagtggct ggtatttgac
    15061 gtgcgtcctt ctggcgcgtc gtttaccggc gagaccattg agcgtgtgaa tatccatacc
    15121 ggcgaggtcg aggttatcta tcgcgtgtca cagggcgcgc acgtcggcgt ggtgaccgtt
    15181 catccaaagt cagagaaata tgtctttatc cacggacctg aaaatcctca tgaaacatgg
    15241 cattacgatt tccatcaccg tcgcggcgtg attgttgaag gcggcaagat gaacaatctc
    15301 gatgcaatgg atattaccgt tccgtacaca tcaggggcgc tgcgtggcgg cagccatgtg
    15361 catgtcttta gcccgaacgg tgaaatggtg agttttacct ataacgacca tgtaatgcat
    15421 cagttcgatt cggcgctgga tttgcgaaac gtcggggttg ctgcaccgtt tggcccggtc
    15481 aacgtacaaa agcagcatcc gcgtgaatac agcggtagcc actggtgcgt gctggtgagt
    15541 aaaaccacgc ccacgccgca gcctggcagc gatgaaatca atcgtgctta tgaagaagga
    15601 tgggtaggaa atcacgcgct ggcgtttatt ggcgacacac tttcgccaaa gggcgagaaa
    15661 gtgccggagc tgtttatcgt tgagttaccg caagatgaag ctggctggaa agcagcaggt
    15721 gatgcgccgt taagtggaac ggaaacaacc ctgcccgcgc caccgcgtgg catcgtgcag
    15781 cgacgtttaa cctttaccca ccatcgggct tatccggggt tagtcaacgt cccgcgccac
    15841 tgggtgcgct gtaatccgca gggtacgcaa atcgcgtttt taatgcgaga tgataacggc
    15901 attgtgcaac tgtggcttat ctcgccacag ggcggcgagc cgcgccagtt aacccataac
    15961 aaaacggata ttcagtctgc atttaactgg catccgtcag gagaatggtt gggctttgtg
    16021 ctgggtaatc gaattgcttg tgcccatgcg caaagcggcg aggttgagta tttaaccgaa
    16081 aaccacgcca atccaccttc tgcggacgcc gtggtcttct cgccggatgg tcagtggctg
    16141 gcgtggatgg aaggcggcca gctgtggatc accgaaactg atcgctaaat cagttatcag
    16201 gcattctggc cggtggaata accgcattgg tggcttgcgc gttggcttcg cttttttcaa
    16261 cgcgtgagcg caccgagctg tctttgcgga acacatccca cggtaataaa agcgtgtcca
    16321 tcacagcggt aaacggcata tcgagaatcg ccagcgactt agtgccccag ttggtttcat
    16381 catcgccaat catggtggcg ctggcgcggg tgccgggata cgttccttct ttccctccgg
    16441 tatgggacat cacgctggag catcctccga gtaatgccat tccgctgcat atcatgaacg
    16501 ctaacaacac atttcttatc ataataaatt catctgttga tcgtgggtgt tggcctgatg
    16561 agttatagcg atcccttgct gaaaataaca tcatcattac gtcgctctgt ggcggctatc
    16621 gcactttaac gtttcgtgct gccccctcag tctatgcaat agaccataaa ctgcaaaaaa
    16681 aagtccgctg ataaggcttg aaaagttcat ttccagaccc atttttacat cgtagccgat
    16741 gaggacgcgc ctgatgggtg ttctggctac ctgacctgtc cattgtggaa ggtcttacat
    16801 tctcgctgat ttcaggagct attgattatg cgtaactttg atttatcccc gctttaccgt
    16861 tctgctattg gatttgaccg tttgtttaac cacttagaaa acaaccagag ccagagtaat
    16921 ggcggctacc ctccgtataa cgttgaactg gtagacgaaa accattaccg cattgctatc
    16981 gctgtggctg gttttgctga gagcgaactg gaaattaccg cccaggataa tctgctggtg
    17041 gtgaaaggtg ctcacgccga cgaacaaaaa gagcgcacct atctgtacca gggcatcgct
    17101 gaacgcaact ttgaacgcaa attccagtta gctgagaaca ttcatgttcg tggtgctaac
    17161 ctagtaaatg gtttgctgta tatcgatctc gaacgcgtga ttccggaagc gaaaaaaccg
    17221 cgccgtatcg aaatcaacta atttcctaag gccgccttgc gcggcctgac atctccatgc
    17281 tcgccgtcag ggagcatatg cgaatcttcg gatttgcagg tacttactcg cttcttagaa
    17341 ggagaaatga ctatgcgtaa cttcgattta tccccactga tgcgtcaatg gatcggtttt
    17401 gacaaactgg ccaacgcact gcaaaacgcc ggtgaaagtc agagcttccc gccgtacaac
    17461 attgagaaaa gcgacgataa ccactaccgc attacccttg cgctggcagg tttccgtcag
    17521 gaagatttag agattcaact ggaaggtacg cgcctgagcg taaaaggcac gccggagcag
    17581 ccaaaagaag agaaaaaatg gctgcatcaa gggcttatga atcagccatt tagcctgagc
    17641 tttacgctgg ctgaaaatat ggaagtctct ggcgcaacct tcgtaaacgg tttactgcat
    17701 attgatttaa ttcgtaatga gcctgaaccc atcgcagcgc agcgtatcgc tatcagcgaa
    17761 cgtcccgcgt taaatagcta actagctatt ctctttgccc cgccattctg gtggggcttt
    17821 ttttgtcttt cttcttcaac aatgcgttaa atttcgactg tttaagatat ttcggcacgt
    17881 tctttcccgt gataatgtgc gctataaggc aaatctgctt caccgattca gccataatcc
    17941 gtagcaatta aaatgttatc tcaagagaaa tggacgatga gtgatatagc attaacggtc
    18001 agtattttgg ctttggtggc agtcgtcggt ttgtttatcg gcaacgtcaa atttcgcggc
    18061 ataggattag gtattggcgg cgtgctgttt ggtgggatca tcgtcggcca ttttgtttct
    18121 caggcgggga tgacattaag tagcgatatg ctgcatgtta ttcaggaatt tggcctgatc
    18181 ctgttcgttt ataccatcgg gattcaggta gggccgggct tctttgcctc attgcgcgtc
    18241 tccggattac gcctcaacct gtttgctgtt ctgatcgtca tcatcggtgg tctggttacc
    18301 gccatcctgc ataaactgtt tgatattcca ctgccggtag tgctggggat tttctccggt
    18361 gcggtaacca atacgccagc gctgggggca gggcagcaga ttttgcgcga cctgggtaca
    18421 ccaatggaaa tggtcgatca gatggggatg agttacgcga tggcgtatcc attcggcatt
    18481 tgcgggatat tgttcaccat gtggatgttg cgggttattt tccgcgtcaa tgtcgagaca
    18541 gaagcccagc agcacgagtc ttcacgcacc aatggcggcg cgctgatcaa gactatcaat
    18601 attcgcgttg agaaccctaa cctgcatgat ttagccatta aagatgtacc gattctcaac
    18661 ggcgacaaaa ttatctgctc gcgtctgaaa cgcgaagaaa ccctaaaagt tccttcgcca
    18721 gataccatta tccaactggg cgatttgctg catctggtgg gtcagccagc ggatttacat
    18781 aatgcgcaac tggtgattgg tcaggaggtc gatacttcgc tgtccacgaa aggcactgat
    18841 ttgcgcgtcg agcgtgtggt ggtcaccaat gaaaacgtgc ttggtaagcg tattcgcgac
    18901 ctgcatttta aagaacgcta tgacgttgtt atctcgcgcc tgaaccgtgc cggggtcgaa
    18961 ctggtcgcca gtggcgatat cagcctgcag ttcggcgata tcctcaacct ggtggggcgt
    19021 ccgtccgcaa ttgatgccgt tgccaatgtg ctggggaatg cgcagcaaaa actgcaacag
    19081 gttcagatgc tgccagtgtt tattggcatc gggcttggcg tattgttagg ttctattccc
    19141 gtctttgtgc caggattccc ggccgcgttg aaactggggc tggcgggcgg tccgctgatt
    19201 atggcgttga tcctcgggcg tatcggcagt atcggcaagc tgtactggtt tatgccgcca
    19261 agtgccaacc tcgcgctgcg ggaactgggg atcgtgctgt tcctctcggt cgttggtctg
    19321 aaatctggtg gggattttgt gaataccctg gtcaatggcg aagggctaag ctggattggt
    19381 tatggtgccc tgatcaccgc cgttccgctg attactgttg gcattctggc gcggatgtta
    19441 gccaaaatga attacctgac catgtgcggg atgctggcag gttccatgac cgatcctccg
    19501 gcgctggcgt ttgctaataa tcttcatcca accagcggtg cggcggcgct ctcttacgcc
    19561 actgtctatc cgttagtgat gttcctgcgc attatcaccc cccaattact ggcggtgctc
    19621 ttctggagta tcggttaact cttgtccggg tggagtcgcc gtaaatggta ctccacctgg
    19681 tattcgctgg catttctgaa catcactgaa taattcaaaa actccccgct ctcgctatat
    19741 gacaacgagg tgatccgcag cagtggcgtg tgttccgcta ccttcatttg ccgcgccagc
    19801 agtctatcgg cgagcaccgg cgtcaggctt tcataattac cgccaatcaa aatcccgcac
    19861 tcttgttcaa tatattcaaa cttcgaccct tccagatgtt gcagcgaaag gttacggaac
    19921 agttttaccg gcatatagct gtcttccagc atcagcggtt tcccttccac aaaacgaacg
    19981 cgacgggaga agtagatctg ctcgttgatt tgaatccgca actggctggc aattgccgga
    20041 ggcgcaggca tgatttcaaa aatcagcacc tggctggtga ctgtttttcc ctgccgtttt
    20101 aacacctcca ccagcccggt caggctggcg gtttgatgca gcacatcttt gcgcaccagg
    20161 taagtgccgc tgccgtggcg gcggaccacc agcccccacg ctaccagcag gtcaatcgct
    20221 ttacggatgg tcatccgcga taccgcaaac tcttccgcca gctttttttc accgggaaga
    20281 aggctgttta gcgtgaaatc cgcggaatta agtcgaattc ttaaccgctc cgcaatgctt
    20341 ttgtagatca tctctagatc tcttttttgc ctgcaagccc gggtaatgcg cccacaagtt
    20401 ctgcttaatc aattgaaaaa acatcttttt tttaaagatg tgttcgatgc tgtgtaccag
    20461 tgctcacaga gatctacttt ttcgcgaaaa agtagatctc taccgcccaa cgaaaagcat
    20521 gaaagcgatc acgaatccca ttgggtcgtc atgttctgcc gatcgcatct tcctatcctc
    20581 gctccaggcc tgccgcataa ccaatcaggc ttcctactta cagaattgag aaaagaggat
    20641 gtggaaatgc tcagtcaaat tcaacgcttt ggcggcgcga tgttcacgcc agtgctgctg
    20701 tttcccttcg ccgggattgt ggtgggtctt gccatcttgc tgcaaaaccc gatgtttgtc
    20761 ggggaatcac tgaccaatcc gaacagttta ttcgcgcaaa tcgtacacat tattgaagag
    20821 ggcggttgga cggtattccg taatatgccg ctgatttttg ctgtcggttt acccattggc
    20881 cttgctaagc aagcgcaggg gcgtgcttgt ctggcggtga tggtgagttt cctgacctgg
    20941 aactatttca tcaatgcgat gggaatgacc tggggaagct acttcggcgt cgatttcact
    21001 caggacgcgg tggcaggtag cggtctgaca atgatggccg ggattaaaac cctcgatacc
    21061 agcattatcg gcgcaattat catttccggc attgtgacgg cgctgcataa ccgtctgttc
    21121 gataaaaaac tgccggtttt tctcggcatt ttccagggga cgtcttatgt ggtgattatc
    21181 gccttcctgg tgatgatccc ctgtgcctgg ctgacattgc tcggctggcc aaaagtacaa
    21241 atggggattg aatctctgca agcgttcctg cgttcggcgg gtgcgcttgg ggtgtgggtt
    21301 tacaccttcc tcgaacgtat tctgatccca accggtttac accacttcat ctacggaccg
    21361 tttatctttg gtccggcagc tgttgaaggc ggaattcaga tgtactgggc gcagcatctg
    21421 caagagttca gtttgagcgc cgagccgctg aaatcgttgt tcccggaagg aggttttgcc
    21481 ctgcacggta actcaaaaat ctttggtgcc gtgggcattt ctttagcgat gtacttcact
    21541 gccgcaccgg aaaatcgggt aaaagtggcg ggtttgctga ttcccgcaac cttaaccgcc
    21601 atgctggtgg gaattaccga accgctggaa tttaccttcc tgttcatttc accgttgctg
    21661 tttgcggtac acgctgtgct tgcggcctca atgtcgaccg tgatgtatct ctttggtgtg
    21721 gtgggcaaca tgggcggagg tctgattgac caggttttac cgcaaaactg gatcccgatg
    21781 ttcagcaacc acgcggatat gatgctgacc caaatcgcca ttgggttgtg ctttaccctg
    21841 ctgtacttcg tggttttccg caccctgatt ctgcaattca acatgtgcac gccgggacgt
    21901 gaagatgcgg aagtgaaact ctactcaaaa gccgaataca aagcctcgcg aggccaaacc
    21961 accgcggcag agccaaaaaa agagctggat caggctgccg gtatcctgca agccctgggc
    22021 ggggtcggca atatctccag cattaacaat tgtgcgacgc gtctacgcat tgcactgcat
    22081 gacatgtcac aaacgctgga tgacgaagtc tttaaaaagc tgggagcgca cggcgtcttc
    22141 cgtagtggcg atgccattca ggtgatcatt ggtctgcatg tatcccagct gcgtgaacag
    22201 ctcgatagct taattaattc tcatcaatca gcagaaaatg ttgccattac ggaggcagta
    22261 taatgaccaa attctcagtg gttgtcgcag gcggtggaag cacctttacg ccaggcatcg
    22321 tgttgatgct cctggcgaat caggaccgtt tcccgcttcg tgcactgaaa ttttatgata
    22381 acgatggttc gcggcaggaa gtgattgccg aagcctgtaa agtcatcctt aaagaaaaag
    22441 cgccggatat tgcgtttagt tacaccaccg atactgaagt ggcgttcagc gacgttgatt
    22501 ttgtcatggc gcacatccgc gtcggcaaat acccgatgcg cgaactggat gaaaaaatcc
    22561 cgctgcgcca cggcgttgtt ggtcaggaaa cttgcggacc cggcggaata gcgtacggca
    22621 tgcgttccat tggcggcgtc ctggaactgg tggattatat ggaaaaatat tcaccaaacg
    22681 cctggatgct caactactcc aacccggcag caattgttgc agaagccacg cgtcgtctgc
    22741 gcccgaatgc gaaaatcctc aacatctgtg acatgccaat cggtattgaa agccggatgg
    22801 cgcaaattgt tgggctgcaa gatcgcaaac agatgcgcgt gcgctactac ggcctgaacc
    22861 actttggctg gtggacatca attgaagatt tgcagggcaa cgacctgatg caccagctgc
    22921 ggcaatatgt ctctaagcac ggttatgttc caccgcagca agatacacat actgaagcga
    22981 gctggaacga cacctatgca aaagcgcggg atgtccaggc actggacccg gatacattgc
    23041 cgaacaccta tctgaaatat tatctcttcc cggattatgt cgttcagcat tccaaccctg
    23101 aacatactcg cgcgaatgag gtaatggaac atcgcgagaa acaggttttc gatgcttgcc
    23161 gcgccattac ggcggcagga aattcagcgg cgggcaagct ggaaattgac gaacatgcgt
    23221 catacatcgt cgatctggcg gcggcaattg ccttcaacac tcaggagcgg atgttgctga
    23281 ttgtgcctaa caacggggca atccataact ttgatgatga agctatggtc gagatcccgt
    23341 gtctggtcgg gcacaacgga ccagaaccac tggtggtcgg cgatatcccg cagtttcaga
    23401 aagggttaat gagtcagcaa gtggcggtgg aaaaactggt cgtggacgcc tgggaacagc
    23461 gttcatatca gcacctgtgg caggcgatta cgttgtcgaa aacggtaccg agcgcctcgg
    23521 tcgccaaagc tattctggat gagttgctgg aggccaacaa agcgtactgg ccagagttac
    23581 gttaattgtg tggactggca tccgttgggt gctggtccgt attctttagc acggctgaag
    23641 gcgcataacc aaatttatgt ttaaaggctt tgctgaaatg aaaggagtcg aagaagttga
    23701 gcgtatcagc gacttcgcca accgagtttc cttgttgctt taatagcgat agcgccagat
    23761 ccagacgcgc atcgaggtaa tattctttcg gcgtctttcc ggtatagcgt aaaaacagac
    23821 gacgcaacca ggcttcgcta caggggatcg tggcagccat atcggctacg ctccagcgtt
    23881 gttgcagact ggcatgtaaa gtggcaatta atttttcaat ttgccgccgt tgtggatctt
    23941 ttttaccgtc tgcgtacata agacaaatcc actgataaat aattttcgtc agaaaagcga
    24001 ctgccagatt attttttatt gcttctggtg aagttattaa ctcagcaact tcggtgagtt
    24061 cctgattata aatttcgcca ttgtaaataa cgctttgctg accgatggga atatccatca
    24121 tactggtggg ggtaaattcc atccagtatt gttcccagac taaaccttca cagtgataag
    24181 agtgaatatc cattggcttt aaaaatataa tacagttgcc atgcagtgtt atttgttcgc
    24241 cattctttaa aagaatcttc ccacatcctt gtacggtata caccgcaacc catgagttgg
    24301 cgataagcgg tttcttcttg tcccgtggcc agatcacccg gtaatgctcg tcggcgtagg
    24361 tatgccagag cgagatcaga gaaattccgc tggagatcac attttcgtta atcaataaag
    24421 gaatattgta tggagtttcg ttttttacat ctgaagtttg caattttcca ttcattttaa
    24481 tatccctccg gcgaattatg ctcacaacct gaacttaaca gaaaacaatc cgttcaggga
    24541 tacgacgttt gtattttaag aatctgactg cctgacccga cgcattttac ctctccctat
    24601 attcatgcgt ccgggacata aacataaata agggctatga gatgaattcg ttacaaatct
    24661 tgagttttgt cggttttacg ctgctggtgg cgatcatcac ctggtggaag gttcgcaaaa
    24721 cagataccgg atcgcaacaa ggctattttc ttgccggacg ttcactaaaa gcgccggtta
    24781 ttgccgcttc gttaatgtta accaaccttt ccacggaaca actggttggt ctttccgggc
    24841 aggcctacaa aagcggcatg tcggtgatgg gctgggaagt gacttcagcg gtgacgctga
    24901 tcttcctcgc gctaatcttt ttaccgcgct atctgaagcg cggcattgcc accatccccg
    24961 attttctgga ggaacgttat gataaaacga cgcgtatgat catcgacttc tgcttcctca
    25021 ttgccaccgg cgtctgcttt ctgccgattg ttctctactc cggcgcgttg gcgctcaaca
    25081 gcctgtttca cgtcggggaa tcgctacaga tttctcacgg tgcggctatc tggctactgg
    25141 taattttgct tggtctggcg ggaattttgt atgcggtgat cggcggactg cgcgcaatgg
    25201 cagtggcgga ctccatcaac ggtattgggc tggttatcgg cgggttgatg gtgccggtat
    25261 ttggcttgat cgcgatgggc aagggcagct ttatgcaggg cattgagcaa attaccaccg
    25321 ttcacgccga gaaattaaac tcagtcggtg gcccgaccga tcccttgccg attggcgcgg
    25381 catttaccgg tttaattctg gtgaacacct tttactggtg tacaaatcag ggcatcgtgc
    25441 aacgcacgct ggcgtcaaaa agcctggcgg aagggcaaaa gggggcgctg ttaacggcgg
    25501 tgctgaaaat gctcgacccg ctggtactgg tgctgccagg gttgattgcg tttcatctgt
    25561 atcaggatct accgaaagcc gacatggcct acccgacgct ggtcaataac gttctgccag
    25621 tgccactggt gggtttcttc ggcgcggtgt tatttggtgc agtgatcagt accttcaacg
    25681 gctttctgaa tagcgccagt acgttattca gtatgggtat ttaccgtcgc atcattaacc
    25741 agaatgccga gccgcagcag ctggtcaccg tcgggcgcaa atttggtttc tttatcgcta
    25801 tcgtttcggt gctggtagcg ccgtggatcg ccaacgcgcc gcaggggctg tatagctgga
    25861 tgaaacagct caacggcatt tacaacgtgc cgctggttac catcatcatt atgggctttt
    25921 tcttcctgcg catcccggcg ctggcggcaa aagtggcgat ggggattggc ataatcagct
    25981 acatcaccat caactatctg gtgaagttcg acttccattt cctctatgtg ctggcctgta
    26041 cgttctgcat caacgtggtc gtgatgctgg tgatcggttt tatcaaaccg cgcgccacgc
    26101 cgttcacctt caaagatgcg tttgcggtgg acatgaaacc gtggaaaaac gtcaagatcg
    26161 cgtcaattgg catcctgttc gcgatgattg gcgtctatgc cgggctggct gaattcggcg
    26221 gctacggtac gcgctggtta gcgatgatca gttatttcat tgccgccgta gtgattgtct
    26281 acctgatttt tgacagctgg cggcatcgtc acgacccagc cgtaaccttt actcccgacg
    26341 cgaaggatag cctatgaaac gccccaattt tctgttcatc atgaccgata cccaggcccc
    26401 caatatggtc ggttgctata gcggtaaacc gctgaatacg caaaatattg atagtctggc
    26461 ggcggaaggt attcgcttta attccgccta cacctgttca ccggtttgta caccggcgcg
    26521 cgccgggctg tttaccggta tctacgctaa ccagtccggg ccgtggacca acaacgtcgc
    26581 gccgggtaaa aacatctcca ctatggggcg ctactttaag gatgccggct atcacacctg
    26641 ttacatcggc aaatggcatc tcgacggtca tgactatttc ggcactggcg agtgtccgcc
    26701 ggagtgggac gctgattact ggttcgatgg ggcgaactat cttagcgaac tgacggaaaa
    26761 agagattagc ctgtggcgca atggcctaaa cagcgtcgaa gatttacagg cgaaccatat
    26821 tgacgaaacc ttcacctggg cgcaccgcat cagcaatcga gcggtggatt ttctgcaaca
    26881 gcccgcgcgc gccgaggaac ccttcctgat ggtggtttcg tatgatgagc cgcatcaccc
    26941 gttcacctgt ccggtggagt atttagagaa atacgctgat ttttactacg agctgggcga
    27001 gaaagcacag gatgacctgg cgaacaaacc agaacatcac cgcttatggg cgcaggcgat
    27061 gccatcgcca gtcggtgatg acgggcttta tcaccatccg ctctattttg cctgtaatga
    27121 ctttgttgat gaccaaatcg gacgggtcat caatgcctta aagccagagc aacgtgaaaa
    27181 tacgtgggtt atttatacct ccgatcacgg cgaaatgatg ggcgcacata agctgatcag
    27241 taaaggggcg gcgatgtatg acgacatcac ccgcattccg ctgatcatcc gttcgccgca
    27301 aggggagcgg cgacaggtcg atacgccagt cagtcatatc gatttactgc cgacaatgat
    27361 ggcgctggca gatattgaaa aaccagagat tctgccgggg gaaaatatcc ttgcagtgaa
    27421 agagccacgc ggcgtgatgg tggaatttaa ccgctacgag attgagcatg acagctttgg
    27481 cggttttatt ccggtgcgtt gctgggtgac ggatgacttt aaactggtgc tcaacctctt
    27541 caccagtgat gaactttacg atcgccgtaa tgatccaaat gaaatgcata acctgatcga
    27601 tgatgcccat tttgccgacg ttcgcagcaa aatgcatgat gccttattgg attatatgga
    27661 caaaattcgc gatccgttcc gcagttacca atggagcctg cgtccgtggc gtaaagatgc
    27721 acagccgcgc tggatggggg catttcgtcc acgcccacaa gatggttatt cgccagtggt
    27781 acgcgactat gacaccggcc tgccgacaca aggggtgaag gtggaagaga aaaaacagaa
    27841 gttctgacga taatgcccgc tgatgaacac ccggcgggca ttattcaggc tgcttaaacc
    27901 agtgaaccgc cagtcaggaa ggaaaagata aggaatatca gcaaaatgct gcacacgatg
    27961 ccgataccaa aaagcaaggt atggaaagcc agcgtcccgc cgccaaaaac ccgcgcgccg
    28021 cgaatgcccc agcgattttc cttacgcata ccgccaataa atgcgcaaag tagcgcagca
    28081 atggccagtg cgataccgct ttgatcgagg attttatcga catccacgtc ctcttttctc
    28141 tccacggtgg taattttttc gccttttatt ccggcgagaa gacctctttt aatttcagcc
    28201 gttttgtccg ccacgatgct ttcaagcgtt ggcggtggtg cggaaaaagg gccgaatgaa
    28261 aagtggataa tccccatcat taaagcgatg gcaccgaata gcatgcccaa tgaactgatt
    28321 ttattttgcg ctatgatacc catctgacct ccttgtagcg gatggcaaaa gcatatcagg
    28381 aaacgggcgg acgaaattct taaaaaaaac cgctaaaggt agagcgttag tgaaattgca
    28441 aaggagcaac aatgaaaatt tcccgcctcg gagaagcgcc tgattaccgc ttctcgctgg
    28501 ctaatgagcg tacttttctg gcgtggatcc gtactgcact aggatttctg gcggcggggg
    28561 ttgggcttga tcaacttgcg ccagatttcg ccacgcccgt cattcgcgaa ctgctggcgt
    28621 tgttgttgtg tctgttttcc ggcgggttag cgatgtatgg ctacttgcgc tggttacgca
    28681 atgaaaaagc gatgcgtctg aaagaggacc tgccctacac caacagctta ttaatcatca
    28741 gcttaatatt gatggtcgta gcagtgatcg ttatgggact ggtgttgtat gccggatagc
    28801 cgcaaagcca gacgcattgc cgacccaggg ctgcagccgg agcgtacatc actggcgtgg
    28861 tttcgtacca tgctgggcta cggcgcgttg atggcgttgg ctatcaaaca caactggcac
    28921 caggcgggca tgttattctg gatttcgatt ggcatcctcg ccatcgtggc gctgatcctc
    28981 tggcactaca ctcgtaatcg caatttaatg gatgtcacga atagcgattt ttcccaattt
    29041 cacgtagtcc gtgacaaatt tttgatctcc ctcgcggtgt tatctctcgc aatactgttt
    29101 gctgtaacgc atatacatca acttatcgta tttattgaga gagtcgcatg acaggaagtc
    29161 aggtcattga tgctgaagag gacaggcata aactggttgt agagtacaag gatgctctgc
    29221 aacctgccga tttttatcac aatttcaaac aacggggcat ccgctccgta cagcttattc
    29281 cccatcttga atttgatgac cggggcgata tgacggccgc ttcggtgaca gcagagctgt
    29341 gggggaaatt tttaattgcc ctgtttgagt gttgggtgcg ggcagatatc agtcgtatct
    29401 cgattgagct ttttgacgcc acattacaaa aatggtgtgg cagcgaaaac ccgcaaccgc
    29461 gccgtggctg ccaggcgtgc gactggcatc tgctgtgtcc gcacgctcgg gaggagacgc
    29521 cagacagcgt gctttgtgcg ggctatcagg cgttttattc ttactctgcg ccgcatatgc
    29581 gggtgatgcg tgatctgata aagcagcatc gttctcccat ggagctgatg acgatgctgc
    29641 ggtgacgtga gcttaaacgg gctgcccctg atgcgacatc cgcgtcgcca gcggtagcca
    29701 gcacagcacg atcaacaatc ccattaaggt catcaacaac cccaggctgc cctgaccggt
    29761 ttgcggcaac atcgcagaga gcgacgccag cacgccggaa ccaatgtttt gtagaccgcc
    29821 gaccagcgcg ccagccgtgc ccgccaggaa agggaacggc tccatcgcgc cgctggtcgc
    29881 cagcggaaac agcatcccgg caccgaaaaa gaacagcgcg gcgggaacga gcagcgtcca
    29941 gacattcatc acgccaaacc agtcggggat ccacatcagt aagccagcca gcaggcagca
    30001 gataaccgac tgccacatca acgttgagaa gcgtttatta ggacgtccgg caaaccatgc
    30061 cccaaaaaat gccgccggaa tcggcagaat aaacaaaata ctgaccgtca tactgctcag
    30121 ccctaacacc gcgcccatca gcacgccgga gcaggcttca aaggcggcaa tcccggccag
    30181 accgccaatc agcatcagca aataacagtt aaaaccgctg ttaccgaaaa gcgttttata
    30241 actggtaagc aggcgcgtgc gcggtgcgtc gaccggacgc gtttccggca tccagcgggc
    30301 catactgaag gtcacaccgg cacaaagtac caacaaaaag agataacagg cgcgccagtt
    30361 ccacatcgta tccagcagac cgccgattag cggtgcgagc aacggactga caagaattcc
    30421 catgtttaac aggctgttag cgtggcgcaa ctgtgtccgt tcatataaat cacgcggcaa
    30481 agtacgcgcc attacgccgc caacgccggt gcccatcccc tgcatcgcgc tggcggcaat
    30541 caatactgtc aaactggagg tcgtgaccgc gaccagcgtt gccagcataa aaatggacat
    30601 tccgacgagg atcaccggtc ggcgacccac acggtcggaa atcgggccat aaaacagctg
    30661 tgagacaccg taagtcagca gataagcgcc cattacgctc tgcaccgccc cttcacgaac
    30721 gttgagatcg cgcgccatat cggcaatagc tggaatataa atggtttgcg ccatctgacc
    30781 gacggccacg agtaatacca acatcaataa caaattgatg tttctatgcc ttttcattat
    30841 cacggatgct tttataaaaa taattagaat aagtcactac gcattcccat aaatgcggga
    30901 gaggaatcta tcatattgat aaatgttagc cagatatacg ccaggaatgg ggaattgttt
    30961 agcggcagaa tatgtaaaca aaagcggcaa taaatgttgc cgggagggat ggtaggtcgg
    31021 ataagatgcg tcagcatcgc atccgacacc aacccacacg ctaaatataa aatgggagcg
    31081 gttacccgct cccctttagt gcgacttgaa tctgaattac ttcaggtatt tcagaacagc
    31141 atcaagcagt tgcagtgctg caacaatgag tttgaggata agaatggcga tatccaccag
    31201 gctcatacgc gtctcctgtg gttcaggagc actgccagct gacgtacctt tccgctgcct
    31261 gttgccagct gttgtgcacg cggtatctca aggagaaacg ttgtgctcgt tgacataaca
    31321 cagtgtgctc acgcggggtt aaggcgctga cggcaccacc cgtttcagcc aggacttagt
    31381 gcgccgggta acgatgcggc ccccgcataa cacattgcgt acagtgatat tatagaagct
    31441 tgctgtataa ataaacagta atatttggac aaaacgcaaa ctgtgatcga tactcaacta
    31501 tgcactaaat acgtcaaaat tcgtgccgaa attgcgcgtt ttgcgcggaa cacgtatact
    31561 ttctgtgttg acataataca gtgtgctttg cggttaccag ccgcgggcgg ctgacgaaac
    31621 ctcgctccgg cggggttttt tgttacctgc agtacagtac aaaacgtgat caacccctca
    31681 attttccctt gctgaaaaat tttccattgt ctcccctgta aagctgtgct tgtataaata
    31741 ttgttaaaca caaaaccaac aaggtcccca atggctactt ccatgctcaa cgcaaaacta
    31801 ctaccaactg cgccatccgc cgcagtggtc gtcgtgcgtg tggtggtggt cgtcggcaat
    31861 gcgccgtagg gactggaaca acacacgatt ccaaaacccc gccggcgcaa accgggcggg
    31921 gtttttcgtt taagcacctc ccggaaagtc ggcccagaag aaaaggactg gagcatggca
    31981 agttcgggca catcatcgac gcgtaagcgc tttaccggcg cagaatttat cgttcatttc
    32041 ctggaacagc agggcattaa gattgtgacg ggcattccgg gcggttctat cctgcctgtt
    32101 tacgatgcct taagccaaag cacgcaaatc cgccatattc tggctcgcca tgaacagggc
    32161 gcggggttta tcgctcaggg aatggcgcgc accgacggta aaccggcggt ctgtatggcc
    32221 tgtagcggac cgggggcgac taacctggtg accgccattg ctgatgcgcg gctggactcc
    32281 atcccgctga tttgcatcac tggtcaggtt cccgcctcaa tgatcggcac tgacgccttc
    32341 caggaagtcg acacctacgg catctctatc cccatcacca aacacaacta tctggtcaga
    32401 catatcgaag aactcccgca ggtcatgagc gatgccttcc gtattgcgca atcaggccgc
    32461 cctggcccgg tgtggataga cattcctaag gatgtgcaaa cggccgtttt tgagattgaa
    32521 gcacagcccg cggtggcaga aaaagccgtc gcgcccgcct ttagcgaaga aagcattcgt
    32581 gacgcagcgg caatgattaa tgctgccaaa cgcccggtgc tttatctggg cggtggtgtg
    32641 atcaatgcgc ctgcgagagt gcgtgaactg gcggagaaag cgcagctgcc taccacaatg
    32701 actttaatgg cgctgggcat gttgccaaaa gcgcatccgt tgtcgctggg tatgttgggg
    32761 atgcacggtg tgcgcagcac caactatatt ttgcaggagg cggatttgtt gattgtgctc
    32821 ggtgcgcgtt ttgatgaccg ggcgattggc aaaaccgagc agttctgccc gaatgccaaa
    32881 attattcatg tcgatatcga ccgcgcagag ctgggtaaaa tcaagcagcc gcacgtagcg
    32941 attcaggcgg atgttgatga cgtgctggcg cagctgatcc cgcaggtgga agcgcaaccg
    33001 cgtgcagagt ggcaccagtt ggtagcggat ttgcagcgcg aattcccgtg tccaatcccg
    33061 aaagcgtgcg atccgttaag ccattacggc ctgatcaacg ccgttgccgc ctgtgtcgat
    33121 gacaatgcga ttatcaccac cgatgtgggt cagcatcaga tgtggacggc gcaggcttat
    33181 ccgctcaatc gcccacgcca gtggctgacc tccggcgggc tgggcacgat gggttttggc
    33241 ctgcctgcgg cgattggcgc ggcgctggcg aacccggatc gcaaagtgtt gtgtttctcc
    33301 ggcgatggca gcctgatgat gaatattcag gagatggcga ccgccagcga aaaccagctg
    33361 gatgttaaaa tcattctgat gaacaacgaa gcgctggggc tggtgcatca gcaacagagt
    33421 ctgttctaca agcagggcgt ttttgccgct acctatccgg gaaaaatcaa ctttatgcag
    33481 attgccgccg gattcggcct cgaaacctgt gatttgaata acgaagccga tccgcaggct
    33541 gcattgcagg aaatcatcaa tcgccctggt ccggcgctga tccatgtgcg cattgatgcc
    33601 gaagaaaaag tgtacccgat ggtgccgcca ggtgcggcga atacagaaat ggtgggggaa
    33661 taagccatgc aaaacacaac tcatgacaac gtgattctgg aactcaccgt tcgcaaccat
    33721 ccgggcgtaa tgacccacgt ttgtggcctt tttgcccgcc gcgcttttaa cgttgaaggc
    33781 attctttgtc tgccgattca ggacagcgac aaaagccata tctggctact ggtcaatgac
    33841 gaccagcgtc tggagcagat gataagccaa atcgataagc tggaagatgt ggtgaaagtg
    33901 cagcgtaatc agtccgatcc gacgatgttt aacaagattg cagtgttttt tcagtaagga
    33961 gcgacattaa accgctcggg ggagttactg atgagcgatt tttgatagat ccgtgcagat
    34021 atatagggtg aaattgtgcc agagaccatc tcaggcacaa tttattagcc caggacgtta
    34081 atttaagtcg cagcaccggt aacctggggg ggacagttag ggctaaaatt ttcagcgatg
    34141 gtgttaaatt ctgattgctg tctataccag tggtcatgac cagcattcta ttaattaatg
    34201 gttcatcgtg aggtatatta tggcaagtcc tataaacagc ggaataatga tggcaaatct
    34261 ctgcccttcc acctttttgg ataaaaatag aaatgtcgct gctgagcttg atattaaaaa
    34321 taacgagaaa aaatattcac ccggcagtaa tttcgcaaaa tggatgttgc aggaaataaa
    34381 gcgcttaata ctaaacatca tgtccggttc tcggagtatt aacaccgata ttcttgacta
    34441 tttccaccct atgccaggca cggaaaataa tggtaatcgt acctgggtgg cggcaactgg
    34501 agaggatgaa tatatcgaaa tcaaacaaac aggtgataag tcttttaata ttacactggt
    34561 tggcagagat aaaccttcac gaaaagaaat tccatattca ggagtggctg tagcgactat
    34621 tattaaatcg ttgtctgaaa aaacttctgc gttagaaaca cattccgctg acacggtttt
    34681 gagga
//
LOCUS       DAZFDC010000035        34590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFDC010000035 DAZFDC010000000
VERSION     DAZFDC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.035"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..626
                     /locus_tag="ACHJ48_001843"
     CDS             <1..626
                     /locus_tag="ACHJ48_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707410.1"
                     /translation="HFRYKFLLSGNAGEMILYFANVFFNPLALSFIIATIICITIKKR
                     SSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGITVTVPSKQWVANIVKQGP
                     TLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHVCDVQGFACAYQEGVQTM
                     SDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKMMLSARQ"
     gene            749..1375
                     /locus_tag="ACHJ48_001844"
     CDS             749..1375
                     /locus_tag="ACHJ48_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707411.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1414..1659)
                     /gene="zapB"
                     /locus_tag="ACHJ48_001845"
     CDS             complement(1414..1659)
                     /gene="zapB"
                     /locus_tag="ACHJ48_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO4707412.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            2085..2930
                     /gene="glpF"
                     /locus_tag="ACHJ48_001846"
     CDS             2085..2930
                     /gene="glpF"
                     /locus_tag="ACHJ48_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO4707413.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2953..4461
                     /gene="glpK"
                     /locus_tag="ACHJ48_001847"
     CDS             2953..4461
                     /gene="glpK"
                     /locus_tag="ACHJ48_001847"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO4707414.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4691..5701
                     /gene="glpX"
                     /locus_tag="ACHJ48_001848"
     CDS             4691..5701
                     /gene="glpX"
                     /locus_tag="ACHJ48_001848"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO4707415.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANSLA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIESGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5798..6544
                     /gene="fpr"
                     /locus_tag="ACHJ48_001849"
     CDS             5798..6544
                     /gene="fpr"
                     /locus_tag="ACHJ48_001849"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO4707416.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVHPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6549..6977)
                     /gene="uspD"
                     /locus_tag="ACHJ48_001850"
     CDS             complement(6549..6977)
                     /gene="uspD"
                     /locus_tag="ACHJ48_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO4707417.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(7004..7303)
                     /gene="yiiS"
                     /locus_tag="ACHJ48_001851"
     CDS             complement(7004..7303)
                     /gene="yiiS"
                     /locus_tag="ACHJ48_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO4707418.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7515..7955)
                     /gene="yiiR"
                     /locus_tag="ACHJ48_001852"
     CDS             complement(7515..7955)
                     /gene="yiiR"
                     /locus_tag="ACHJ48_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4707419.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            8056..8655
                     /gene="yiiQ"
                     /locus_tag="ACHJ48_001853"
     CDS             8056..8655
                     /gene="yiiQ"
                     /locus_tag="ACHJ48_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO4707420.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8763..9530
                     /gene="tpiA"
                     /locus_tag="ACHJ48_001854"
     CDS             8763..9530
                     /gene="tpiA"
                     /locus_tag="ACHJ48_001854"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO4707421.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9585..10340)
                     /gene="cdh"
                     /locus_tag="ACHJ48_001855"
     CDS             complement(9585..10340)
                     /gene="cdh"
                     /locus_tag="ACHJ48_001855"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO4707422.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10447..11436)
                     /gene="sbp"
                     /locus_tag="ACHJ48_001856"
     CDS             complement(10447..11436)
                     /gene="sbp"
                     /locus_tag="ACHJ48_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO4707423.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKLGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLISWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11755..12717)
                     /gene="pfkA"
                     /locus_tag="ACHJ48_001857"
     CDS             complement(11755..12717)
                     /gene="pfkA"
                     /locus_tag="ACHJ48_001857"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO4707424.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12898..13800)
                     /gene="fieF"
                     /locus_tag="ACHJ48_001858"
     CDS             complement(12898..13800)
                     /gene="fieF"
                     /locus_tag="ACHJ48_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO4707425.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(13949..14449)
                     /gene="cpxP"
                     /locus_tag="ACHJ48_001859"
     CDS             complement(13949..14449)
                     /gene="cpxP"
                     /locus_tag="ACHJ48_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO4707426.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            14599..15297
                     /gene="cpxR"
                     /locus_tag="ACHJ48_001860"
     CDS             14599..15297
                     /gene="cpxR"
                     /locus_tag="ACHJ48_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO4707427.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            15294..16667
                     /gene="cpxA"
                     /locus_tag="ACHJ48_001861"
     CDS             15294..16667
                     /gene="cpxA"
                     /locus_tag="ACHJ48_001861"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO4707428.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(16773..17447)
                     /gene="yiiM"
                     /locus_tag="ACHJ48_001862"
     CDS             complement(16773..17447)
                     /gene="yiiM"
                     /locus_tag="ACHJ48_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO4707429.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(17596..18579)
                     /gene="kdgT"
                     /locus_tag="ACHJ48_001863"
     CDS             complement(17596..18579)
                     /gene="kdgT"
                     /locus_tag="ACHJ48_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO4707430.1"
                     /translation="MQIKRLIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(18839..19459)
                     /gene="sodA"
                     /locus_tag="ACHJ48_001864"
     CDS             complement(18839..19459)
                     /gene="sodA"
                     /locus_tag="ACHJ48_001864"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO4707431.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            19744..20778
                     /gene="rhaT"
                     /locus_tag="ACHJ48_001865"
                     /pseudo
     CDS             19744..20778
                     /gene="rhaT"
                     /locus_tag="ACHJ48_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(20775..21623)
                     /gene="rhaR"
                     /locus_tag="ACHJ48_001866"
     CDS             complement(20775..21623)
                     /gene="rhaR"
                     /locus_tag="ACHJ48_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO4707432.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(21697..22533)
                     /gene="rhaS"
                     /locus_tag="ACHJ48_001867"
     CDS             complement(21697..22533)
                     /gene="rhaS"
                     /locus_tag="ACHJ48_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO4707433.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDITYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            22821..24290
                     /gene="rhaB"
                     /locus_tag="ACHJ48_001868"
     CDS             22821..24290
                     /gene="rhaB"
                     /locus_tag="ACHJ48_001868"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO4707434.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALISATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRQTKELCA"
     gene            24287..25546
                     /gene="rhaA"
                     /locus_tag="ACHJ48_001869"
     CDS             24287..25546
                     /gene="rhaA"
                     /locus_tag="ACHJ48_001869"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO4707435.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWIIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKETLSRRG"
     gene            25721..26545
                     /gene="rhaD"
                     /locus_tag="ACHJ48_001870"
     CDS             25721..26545
                     /gene="rhaD"
                     /locus_tag="ACHJ48_001870"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO4707436.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAEVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            26555..26869
                     /gene="rhaM"
                     /locus_tag="ACHJ48_001871"
     CDS             26555..26869
                     /gene="rhaM"
                     /locus_tag="ACHJ48_001871"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO4707437.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(26910..28304)
                     /locus_tag="ACHJ48_001872"
     CDS             complement(26910..28304)
                     /locus_tag="ACHJ48_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO4707438.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLMLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKMDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCCIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            28782..29228
                     /gene="frvA"
                     /locus_tag="ACHJ48_001873"
     CDS             28782..29228
                     /gene="frvA"
                     /locus_tag="ACHJ48_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO4707439.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSTCVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            29239..30678
                     /gene="frvB"
                     /locus_tag="ACHJ48_001874"
     CDS             29239..30678
                     /gene="frvB"
                     /locus_tag="ACHJ48_001874"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO4707440.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYAL
                     AAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLA
                     LNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            30668..31738
                     /gene="frvX"
                     /locus_tag="ACHJ48_001875"
     CDS             30668..31738
                     /gene="frvX"
                     /locus_tag="ACHJ48_001875"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4707441.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAKKRGAEIGDFISPEAN
                     FACWGEDKIVGKALDNRIGCAMMAELLQTVNNPGITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            31738..33486
                     /gene="frvR"
                     /locus_tag="ACHJ48_001876"
     CDS             31738..33486
                     /gene="frvR"
                     /locus_tag="ACHJ48_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO4707442.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHNGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGLASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(33536..>34590)
                     /gene="yiiG"
                     /locus_tag="ACHJ48_001877"
     CDS             complement(33536..>34590)
                     /gene="yiiG"
                     /locus_tag="ACHJ48_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO4707443.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETPLPANSQPAAPAPE
                     AKPTEAPVAKADAKPETPAQPLVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADWL
                     KDFKQGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVA
                     LGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQ
                     LAELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAK
                     EADKGGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPR
                     ALREYNEMVDDYNSLR"
BASE COUNT         8545 a         8830 c         8624 g         8591 t
ORIGIN      
        1 accatttccg ctacaaattt cttttatctg gcaacgcggg tgaaatgatt ttatattttg
       61 ctaacgtatt ttttaaccca cttgccttat catttattat cgccacaatc atctgtataa
      121 cgataaaaaa aagaagcagc cgcagcttta ttcgcggcac ttgttggtta atgggattat
      181 ttcttatcta tagcagctat acggtgtggg aacgacacag catatgggat tatactttcc
      241 cggagccagg tatcaccgtc acggttccga gcaaacagtg ggttgccaat atagtaaaac
      301 aaggaccaac cctggtgacc cgggatgccc atgctatttt agctattgtt gccttctcac
      361 aaaatgaact cggtgtgcat tcaatcgagg aacttaccgc gactcaaaac ggaactgcag
      421 aaatgcatgt ttgtgatgtt cagggatttg cctgcgctta tcaggaagga gtgcaaacaa
      481 tgagtgatgg caaagtaaga catgctattt tcatgactct actggataac actaatttga
      541 tacaactggt cgcagctatc gacccagatt atttggacga atatcaagaa gaagtaatga
      601 aaatgatgct ttctgctcgt caatgatctg atgtgcatgt taataataat ggattgatat
      661 gagatacgtt atcttattaa tactatggat aataactgtt tttcttagtc atcttcagca
      721 cagtaacttg cttactggag atccagggat ggttggcgag tatatcggtt cagtacttct
      781 tggaccgtta ttacttcccg tgcttgtgtc aggtattctg tgcacattca ctaaaaaaac
      841 gcgtaacttc gccagtttta ctcgcgggtg ctgttgggta ttgggagtcc tgcttttatc
      901 caacgtcggc aatacgtttc gtctgttcac cccgtggcac tatacgtttg agaaggcagc
      961 tatttccgtg actgtcccaa accgacactg gaatacggtt tctattagta ctgataaaac
     1021 cattgatata agaagtgaag ataatagcgt ctttatttct gctttccgcc tgcccgcagg
     1081 acgtagcgcc gatgactcgc tggaagagtt aaagaagatg cagcgagaca atctcaagga
     1141 tcaatacaat gaagaaacct tccagtttca tgattgtaat gccaaacatt tcacctgtaa
     1201 gtatcaggat gtgttgataa attttgatgg tcagcagaaa agaaccatca gtgtttatct
     1261 tgaagacacc cccagggctg tggggatcat tgcattaatg gagccagata cggcagataa
     1321 atatcgccaa caggcgatgg agattatgtt gtctgctaaa aatacagtga agtaaaaaat
     1381 aaggcgcctt gcggcgcctc tgcgtggaag agattaaacc tcttccatgc gacccagcag
     1441 ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc
     1501 cagctcttcg cgctgatgct gggcattttg aacttcctgc gacaatgagt tgtttttttc
     1561 tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt
     1621 tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc
     1681 gttgatggat aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca
     1741 ctttagttag ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag
     1801 attacggttt gccacacttt tcatccttct cctggtgaca taatccacac caatcgaaaa
     1861 tgttaataaa tttgttgcgc gaatgttcta acaaacatgc atcatgtaca atcagatgga
     1921 ataaatgacg cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt
     1981 ctcgtttttg ctcgttaacg ataagtttac agcatgccta caagcatcgt ggaggtccgt
     2041 gactttcacg catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa
     2101 ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg
     2161 tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg
     2221 tcatttgggg actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc
     2281 atcttaatcc cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag
     2341 ttattccttt tatcgtttca caagttgccg gcgctttctg tgctgcggct ttagtttacg
     2401 ggctttacta caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg
     2461 ttgaaagtgt tgatctggca ggcactttct ctacttaccc taatcctcat atcaattttg
     2521 tgcaggcttt cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt
     2581 taacggacga tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac
     2641 tgattgcggt cattggcgca tctatgggcc cattgacggg ttttgccatg aacccagcgc
     2701 gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc gcctttaccg
     2761 gcggcagaga cattccttac ttcctggtgc cgctttttgg ccctatcgtt ggcgcgattg
     2821 taggtgcatt tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg
     2881 tggaagaaaa ggagaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg
     2941 ggacaattaa acatgactga aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc
     3001 tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt
     3061 gagcaaatct acccaaaacc aggctgggta gaacacgacc caatggaaat ctgggccacc
     3121 caaagctcca cgctggtaga agtgctggcg aaagccgata tcagttccga tcaaattgca
     3181 gctatcggta ttacgaacca gcgtgaaacc actattgtct gggaaaaaga aaccggcaag
     3241 cctatctata acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta
     3301 aaacgtgacg gtttagaaga ttatatccgc agcaataccg gtctggtgat tgacccgtac
     3361 ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg cgagcgtgcg
     3421 cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc ttatctggaa aatgactcag
     3481 ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta ccatgttgtt caacatccat
     3541 accctggact gggacgacaa aatgctggaa gtgctggata ttccgcgcga gatgctgcca
     3601 gaagtgcgtc gttcttccga agtgtacggt cagactaaca ttggcggcaa aggcggcacg
     3661 cgtattccaa tctccgggat cgccggtgac cagcaggccg cgctgtttgg tcagttgtgc
     3721 gtgaaagaag ggatggcgaa gaacacctat ggcactggct gctttatgct gatgaacact
     3781 ggcgagaaag cggtgaaatc agaaaacggc ctactgacca ccatcgcctg cggcccgact
     3841 ggcgaagtga actatgcgtt ggaaggtgcg gtgtttatgg caggcgcatc cattcagtgg
     3901 ctgcgcgatg agatgaagtt gattaacgac gcctacgatt ccgaatattt cgccaccaaa
     3961 gtgcaaaaca ccaatggtgt gtatgtggtt ccggcattta ccgggctggg tgcgccgtac
     4021 tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc gtggggtgaa cgctaaccac
     4081 attatacgcg cgacgctgga gtctattgct tatcagacgc gtgacgtgct ggaagcgatg
     4141 caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg atggtggcgc agtagcaaac
     4201 aatttcctga tgcagttcca gtctgatatt ctcggcaccc gcgttgagcg cccggaagtt
     4261 cgcgaagtca ccgcattggg tgcggcctat ctcgcaggcc tggcggttgg cttctggcag
     4321 aacctcgacg agctgcaaga gaaagcggtg attgagcgcg agttccgtcc aggcatcgaa
     4381 accactgagc gtaattaccg ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggcg
     4441 tgggaagaac acgacgagta atgtcaggca cctgaatcga ttgcctgatg ctgatcgcct
     4501 gatgcgacgc ttacgcgtct tatcaggcct acgagccaat gccattttgt gtaggcggga
     4561 taaggcgttc acgccgcatc cggcatcacg cagactctga aaccgtgcgg gggcaacccc
     4621 gcacacatca ataatcccac ccttcccctg tgctacactt cgcgccattc cttactgctt
     4681 agagtttgct atgagacgag aacttgccat cgaattttcc cgcgtcaccg aatctgcggc
     4741 gctggctggc tacaaatggt taggacgcgg cgataaaaac accgcggacg gcgcggcggt
     4801 aaacgccatg cgtattatgc tcaaccaggt caacattgac ggcaccatcg tcattggtga
     4861 aggtgaaatc gacgaagcac cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg
     4921 cgacgcggta gatattgctg ttgatccgat tgaaggcacg cgcatgacgg cgatgggtca
     4981 ggctaactcg ctggcggtgc tggcagtagg cgataaaggc tgtttcctca acgcgccaga
     5041 tatgtatatg gagaagctga tcgtcggacc gggagccaaa ggcaccattg atctgaacct
     5101 gccgctggcg gataacctgc gcaatgtcgc ggcggcgctc ggcaaaccgt tgagcgaact
     5161 gacggtaacg attctggcta aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact
     5221 cggcgtacgc gtatttgcta ttccggatgg cgacgttgcg gcctcaattc tcacctgtat
     5281 gccagacagc gaagttgacg tgctgtacgg aattggtggc gcgccggaag gcgtggtttc
     5341 tgcggcggtg atccgcgcat tagatggcga catgaacggt cgtctgctgg cgcgtcatga
     5401 cgtcaaaggc gacaacgaag agaatcgtcg cattggcgag caggagctgg cacgctgcaa
     5461 agcgatgggc atcgaatccg gtaaagtatt gcgtctgggc gatatggcgc gcagcgataa
     5521 cgtcatcttc tctgccaccg gtattaccaa aggcgatctg ctggaaggca ttagccgcaa
     5581 aggcaatatc gcgactaccg aaacgctgct gatccgcggc aaatcacgca ccattcgccg
     5641 cattcagtct atccactatc tggatcgcaa agacccggaa atgcaggtgc acatcctctg
     5701 attgatttga tcgattgagc cttccagtcc ttcgggactg gaattttttt gttcggagaa
     5761 cgaagataag gcaattcaat caaaacagga gaaaaacatg gctgattggg taacaggcaa
     5821 agtcactaaa gtgcagaact ggaccgacgc cctgtttagt ctcaccgttc acgcccctgt
     5881 gcatccgttt accgccgggc aatttaccaa gcttggcctt gaaatcgacg gcgaacgcgt
     5941 ccaacgcgcc tactcctatg ttaactcgcc cgataatccc gatctggagt tttacctggt
     6001 caccgtcccc gatggcaaat taagcccacg attggcggca ctgaaaccgg gcgatgaagt
     6061 gcaggtggtt agcgaagcag ctggcttctt tgttctggat gaagtaccag attgcgaaac
     6121 gctatggatg ctggcaaccg gtacagcgat tggcccttat ttatcgattc tgcaactagg
     6181 taaagattta gatcgcttca aaaatctggt cctggttcac gccgcacgtt atgccgccga
     6241 cttaagctat ttgccactga tgcaagaact ggaaaaacgc tacgaaggca aactgcgcat
     6301 tcagacggtg gtcagtcggg aaacggcagc ggggtcgctc accggacgga taccggcatt
     6361 aattgaaagt ggtgaactgg aaagcgcgat tggcctgccg atgaataaag aaaccagcca
     6421 tgtgatgctg tgcggcaatc cacagatggt gcgcgatacg caacagttgc tgaaagagac
     6481 ccggcagatg acgaaacatt tacgtcgccg accgggtcac atgacagcgg agcattactg
     6541 gtaagcggtt acttatcgat aaacggcacg atgagcaaat ccgcactcaa cttattgatc
     6601 atcccgcgat atgccggcat caaacggttg ataaatgagt ggtgatgacc acagacaagg
     6661 aggtcgcact gctctttttg cataatttcc agcagtgttt ccggcatttc tccgcgttca
     6721 atacgcagtt ttgtcttcgg ccattgaata tttttcgtca gtttatacag cttgttatcc
     6781 gacttattct tcaacaattg aagaatatct tctgttgcag ggaagtagat acccggatac
     6841 aactcgctta agccatcatc aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg
     6901 agctccaagg ctttattcac cagtaaggca tcttcttcat tcccggaaat tgccacgcca
     6961 atgtgtttat aagccatgtt taactccttc taaagcaact ccatcaagct aacgaacgca
     7021 gtgataactc aaaaataatc atctcggcct ggcatgagaa ggtgaaggcc gcatcaagct
     7081 caacttgccc ctccagatcc ttaatcttat aggccacgct ggccccttca tctgcagagg
     7141 atgcagcggc ggcaagctct ttcagtttgg tcataaagga ctctgcttct tcgcgggtgg
     7201 caaaaaagcg cgaaaactta ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa
     7261 tcgcacatcc tttagtactg catttatcta cgacatcttt catagggcac ctctgtatat
     7321 tcgcctctct gtcgattacg acttaagaat aatgcgccgt taagggaaga acaaacaagt
     7381 aatgatctta tggattacca ttttttttga tattgcgtaa ggtgaagagt tcagaaggat
     7441 tttccgggta ttgtcatttg catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag
     7501 agatatatta aaggttaatt acttgattta ttgtcggctt tatacttcac atcctgagta
     7561 tctttaccat atttattttc gccttgtgtg ccaacaaacg caccaagatc gataagcatc
     7621 atcaccagaa tcaacgtcgg gacaaaacgc cctaccgccc attgccagac acccggtaaa
     7681 atcgcccagt tacccgccag tagcatccac gccacaatca tcagaaatgc ccatgcgccg
     7741 gaacgcccgc gatcatgcaa gcgcttaacg gttactgccg ctgttggcca gagcaagcac
     7801 acaaggcaaa acgccgcggt ctgaatatcg agtaaatttt tacccgccag tgagaaaagc
     7861 accagcatgc ctgcgaacca caggcctatc caaatccaga aatcacggcg tccaatacgc
     7921 cctttaaatg agaataacca ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt
     7981 ccaggattct acccttttga caagggcgac agatatcgtt ttaatcggag ccagtcataa
     8041 caaaaggtac tgtcaatgaa gccagggtgt acgctgtttt ttctcttatg ttctgcatta
     8101 accgttacaa caacggcgca tgcacaaaca ccagatacgg caacgaccgc gccttatctg
     8161 ctggctggag cccctacttt cgatctctcc atcagccagt ttcgagaaga ctttaacagc
     8221 cagaatccca gcctgccact gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc
     8281 aatctcactc gtgctgccag taaaattaac gagaacttgt atgcttctac agcgctggag
     8341 cgcggtacct taaaaatcaa aagcattcaa atgacatggc tacccatcca ggggccagag
     8401 caaaaagccg cgaaagcgaa agctcaggaa tacatggcag cggtgatccg cacactcacc
     8461 ccattaatga ccaaaacaca aagccagaaa aaactgcagt cgctactaac ggcggggaaa
     8521 aacaaacgtt attacaccga gacagaaggt gcactgcgct atgttgtcgc ggacaacggc
     8581 gaaaaggggc tgaccttcgc tgttgaaccg attaagctgg cgctatctga atcgcttgaa
     8641 ggtttgaata aatgacaaaa agcaaagcct ttgtgccgat gaatctctat actgtttcac
     8701 agacctgctg ccctgcgggg cggccatctt cctttattcg cttataagcg tggagaatta
     8761 aaatgcgaca tcctttagtg atgggtaact ggaaactgaa cggcagccgc cacatggttc
     8821 acgagctggt ttctaacctg cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa
     8881 tcgcaccacc ggaaatgtac atcgatatgg cgaagcgtga agctgaaggc agccacatca
     8941 tgctgggtgc gcaaaacgtg gacctgaacc tgtccggcgc attcaccggt gaaacctctg
     9001 ctgctatgct gaaagacatc ggcgcacagt acatcatcat cggtcactct gaacgtcgta
     9061 cttaccacaa agaatctgac gaactgatcg cgaaaaaatt cgcggtgctg aaagagcagg
     9121 gcctgactcc ggttctgtgc atcggtgaaa ccgaagctga aaacgaagcg ggcaaaactg
     9181 aagaagtttg cgcacgtcag atcgacgcgg tactgaaaac tcagggtgct gcggcattcg
     9241 aaggtgcggt tatcgcttac gaacctgtat gggcaatcgg tactggcaaa tctgcaactc
     9301 cggctcaggc acaggctgtt cacaaattca ttcgtgacca catcgctaaa gttgacgcta
     9361 acatcgctga acaagtgatc attcagtacg gcggctctgt aaacgcgtct aacgctgcag
     9421 aactgtttgc tcagccagac atcgacggcg cgctggttgg cggtgcgtct ctgaaagctg
     9481 acgctttcgc agtaatcgtt aaagctgcag aagcggctaa acaggcttaa gtctgacagg
     9541 tgccggattt catatccggc acttactttc cttaactctt cgccttaacg caaaatctca
     9601 cactgatgat cctgaatttc ctcggctgaa gcacggttaa gcgtcagtag atttcgttgt
     9661 gtcgccagca atacaaatga gttatcactc tgccgtacca tcgccagccc gtagcttccc
     9721 atatgttccc gcgcctcagg tacttcttct gccagcatca taaatgggct gcgttgtacc
     9781 agttcgcttt ccgttacccg acgcgccagg tattcatgcc cgcgcaaacc acctggcagt
     9841 ggcaaccagc ggctgctgat gttcgccaga ttgttatcca gctgttcgcg cacatcagga
     9901 cgaatacaag agatatgaat atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc
     9961 gccaaagaaa ccgcgcgatc gggaaccggc tggccgtatt ttttgctcat aaaatcacgc
    10021 gcctgccagg ccaaccaaaa gaagttcggc gttgaaggat cggtcaacaa aggactttca
    10081 gtaccgttaa tacgatacgt tggcatcaac agatattgca gtgggccatt aagatctttt
    10141 aaaaccacgt atccggcatt gggtttgact tccgcacatg gcgaaggatt ttgattttgc
    10201 tgctgattgg gcaaacattc ctcaaggaca atcttacgta atgtatccga ctcttcaccg
    10261 gttaatttcc agtaaccaat accggcagcc acaacggcga taactatcat caccaaaaaa
    10321 agaagacccg cttttttcat ctttttttcc ctgtacctca aagagatgca agggtaacgc
    10381 aaaatcgtga caaataaaaa acccggtcgc gagaaaccac cgggtttgat aattatcctg
    10441 gggaaatcag cgtttgctga tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg
    10501 cgctttcgtc cagccgccga actcttcatc aatggtgaat aacttcagct ttggaaacgc
    10561 attttcgtac tttttcgcca cctcagcgtc gcgcggacgg tagtagtttt tcgcggcaat
    10621 ttcctgacct tctggcgagt agagatattt caggtaggct tccgccacct ctttagtacc
    10681 ttttttctcg accactttat cgaccaccga cacggttggc tctgcgagga tagactcact
    10741 cggcgtgacg atttcgaatt tatctttccc cagttcattc gctgccagca gagcttcgtt
    10801 ttcccaggaa atcagtacat cgccaattcc gcgctcgaca aaagtgttag tggagccgcg
    10861 cgcgccagaa tccagaactt cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt
    10921 tgcctgatcg ttgttgttgt gatgcagcgc gtagccccag gctgccaggt agttccagcg
    10981 cgcgccacca gagcttttcg gattaggcgt gatcaccgaa acacccagtt taatcagatc
    11041 gttccagtca tggatctgct tcggatttcc cttacgtacc aggaaaacaa tggtggaagt
    11101 gtacggtgcg gagttatccg gcagacgttt gatccactct ttatcaatcc gcccgcgttc
    11161 cgcaatcgcg tccacgtcat aggccagagc cagcgtgaca acatcagctt caataccgtt
    11221 gattaccgac gttgcttgtt tgcctgaacc accgtgtgac tgacgaatca ccacgttatc
    11281 gccagtttgc tgtttccagt gggcgctgaa tgccttgttg tactgttcgt acaattcgcg
    11341 cgttggatca tatgaaacgt taagaagctg aatatccttt gccataacgc tggttgccgc
    11401 cagcaaaaat gttaacccta cgccccactt gttcatcgcc cgactctctt atgttgtgtt
    11461 gtgatgagca aagcgtgcca gaaggttaac caaacattaa agaataaaaa aagattggct
    11521 ataacttgcg ggtatatgtt gggggttaaa aaggcggaag aaatccgcct catattgctg
    11581 acaaagtgcg ctttatcgat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt
    11641 ttgtaaattc aataaattgt agaattcacg taggcctgat aagcgaagcg catcaggcat
    11701 ttttgcttct gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattagta
    11761 cagttttttc gcgcagtcca gccagtcgcc tttgaacgga cgcttcatgt tttcgatagc
    11821 gtcgatgatg tcgtggtgaa ccagctgttc gttctgtata cctacgcaac gaccgccgta
    11881 acctgccagc agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta
    11941 aggcaccgga gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc
    12001 ggtttctttc tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc
    12061 cacgatcgcg tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc
    12121 acggctgaat tcaacttccg gaaccacaac gaattcacag ccaccggcaa tggccgcagc
    12181 cagcgtcaga tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga
    12241 agaagaggtg tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa
    12301 accgatagtg tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca ggccgatgca
    12361 cgggaagccc atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac
    12421 caccagcgca tcgatcccac gttttttcag gttttcgata gccacggcgc ggatgttctc
    12481 gtcgcggaat tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc
    12541 agaaacgctg taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc
    12601 ataaatgccc attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc
    12661 ggcgttcatg cctggcgcat caccgccgct tgtcaacaca ccgattttct taatcatgac
    12721 tacctctgaa ctttggaatg caaaataaaa tctgttgccg gaagtcttct tgcacatcga
    12781 agtgatccaa cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc
    12841 aggccaaatg gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta
    12901 tgaaagcata gaccgtttac cctccctagg tacgacggaa caggggtcct gatggataat
    12961 cacatccgat cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg
    13021 tgcctgaacc aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg
    13081 ccctgactgc cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat
    13141 atcaataatt tcttgccgtt cctcatcagg taatgcgcga tccagtaatg actgtaccgc
    13201 ctcatatccc atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag
    13261 agcatcggcg cgatgccagc cgtaccagga caaccccagc gccagcagaa ttgcgccgtt
    13321 catcataaca tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg
    13381 gcgcaccacc caacgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt
    13441 cacgataacc ccgacgcctg gatctgtcat cggtgttgga gatatcagat gttgaatgcc
    13501 tgtcaaaaac aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag
    13561 ggactcagct ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg
    13621 caccaccaat aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc
    13681 gagaatactc accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcagtga
    13741 agccatcgcc gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat
    13801 aaatactccc gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta
    13861 agcagcaggc aaattgagga taaaaaaaac ccccacatca tgtgggggaa gacagggatg
    13921 gtgtctatgg caaggaaaac agggtttact actgggaacg tgagttgcta ctactcaata
    13981 gcttcaacga tgaacttttt tgccattgcg tcacgtcacg caactgctcc attcgttgtt
    14041 gatgtttctc gtttaaaacc gcttgctgct ccggcgttaa caggcgatac atttggttgc
    14101 ggacttttgc catctcaacc tgacgagcaa tttgctcatt cgccattttt tctgcctgtg
    14161 cgcgcacagc gttttcatca aaattttctg cggtgacaag acgatgcatt gtctccagtt
    14221 cgctaacatt aacaggaggc tgttcgtgcc gggcctgttg cataagatct cgcatctgct
    14281 gacgctgatg ttcggttaaa cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg
    14341 taagttcttc acccggatgc cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg
    14401 aactgactgc cagcgttgag gccatgacgg cagcggtaac tatgcgcatc atttgctccc
    14461 aaaatctttc tgtcgcgatt caacgataga gagtttacga ttcaggctgc aaacatgcgt
    14521 cagggggtgt aaaacaacgt aaagtcatgg attagcgacg tctgatgacg taatttctgc
    14581 ctcggaggta tttaaacaat gaataaaatc ctgttagttg atgatgaccg agagctgact
    14641 tccctattaa aggagctgct cgagatggaa ggcttcaacg tgattgttgc ccacgatggg
    14701 gaacaggcgc ttgatcttct ggacgacagc attgatttac ttttgcttga cgtaatgatg
    14761 ccgaagaaaa atggtatcga cacattaaaa gcacttcgcc agacacacca gacgcctgtc
    14821 attatgttga cggcgcgcgg cagtgaactt gatcgcgttc tcggccttga gctgggcgca
    14881 gatgactatc tcccgaaacc gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc
    14941 ctgcgccgtt cgcactggag cgagcaacag caaaacaacg acaacggttc accgacactg
    15001 gaagttgatg ccttagtact gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg
    15061 ctggagttaa ccggtactga gtttaccctg ctctatttgc tggcacagca tctgggtcag
    15121 gtggtttccc gtgaacattt aagccaggaa gtgctgggca aacgcctgac gcctttcgac
    15181 cgcgctatcg atatgcacat ttccaacctg cgtcgtaaac tgccggatcg taaagatggt
    15241 cacccgtggt ttaaaacctt gcgtggtcgc ggctacctga tggtttctgc ttcatgatag
    15301 gcagcttaac cgcgcgcatc ttcgccatct tctggctgac gctggcgctg gtgttgatgt
    15361 tggttttgat gttacccaag ctcgattcac gccagatgac cgagcttctg gatagcgaac
    15421 agcgtcaggg tctgatgatt gagcagcatg ttgaagcgga gctggcgaac gatccgccca
    15481 acgatttaat gtggtggcgg cgtctgttcc gggcgattga taagtgggca ccgccaggac
    15541 agcgtttgtt attggtgacc accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc
    15601 agatcattcg taactttatt ggtcaggccg ataacgccga tcatccgcag aagaaaaagt
    15661 atggccgcgt ggaactggtc ggtccgttct ccgtgcgtga tggcgaagat aattaccaac
    15721 tttatctgat tcgtccggcc agcagttctc aatccgattt cattaactta ctgtttgacc
    15781 gcccgctatt actgctgatt gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg
    15841 cctggagtct ggcaaaaccg gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg
    15901 gaaacttacg ccagcacccg gaactggaag cggggccaca ggaattcctt gccgcaggtg
    15961 ccagttttaa ccagatggtc accgcgctgg agcgcatgat gacctctcag cagcgtctgc
    16021 tttctgatat ctctcacgag ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt
    16081 tactgcgccg tcgtagtggt gaaagcaagg aactggagcg tattgaaacc gaagcgcaac
    16141 gtctggacag catgattaac gacctgttgg tgatgtcacg taatcagcaa aaaaacgcgc
    16201 tggttagcga gaccatcaaa gccaatcagt tgtggagtga agtgctggat aacgcggcgt
    16261 tcgaagccga gcaaatgggc aagtcgttga cagttaactt cccgcctggg ccgtggccgc
    16321 tgtacggcaa cccgaacgcc ctggagagtg cgctggaaaa cattgttcgt aatgccctgc
    16381 gttattccca tacgaagatt gaagtgggct ttgcggtaga taaagacggt atcaccatta
    16441 cggtggacga cgatggtcct ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt
    16501 tctatcggac cgatgaagcg cgcgatcgtg aatctggcgg tacaggtttg ggactggcga
    16561 ttgttgaaac cgccattcag cagcatcgtg gctgggtgaa agcagaagac agcccgctgg
    16621 gcggtttacg gctggtgatt tggttgccgc tgtataagcg gagttaaact ccgcatttgt
    16681 aggcaggata aggcgtttac gccgcatccg gcatttgagc aggatgcctg atgcgacgct
    16741 gatagcgtct tatcaggcct acactccggg ggttattttc cccacaaccg gcgggaaaaa
    16801 tcttcgatct tgccgctcag tcggcgcttt tgcatcgttc gcgtccagct tttcgataac
    16861 ccggcagcgg agagtaaacg gtgatactga tcgtcatcaa acggcatatg ccatgcgatg
    16921 gcagcagcct cctgcacggt cacatcgctg acacgggaaa ccaactccag cggcgcgtcc
    16981 gcagacactt tccctggcgc tatcacgcta tacaaccatc ccactttacc cgtgttttgc
    17041 atcaactgcg ctatatcgct gatatcaaaa tggtaattga gtttatagca aggcgagcgc
    17101 ggctggctga cctgaattaa tgcctctccc cagcggaaaa tatcgccaat ataaacatta
    17161 ctttccgtga gaccgtcggt tgagagattt tcgccaaacg caggcgcaac aaacaactcc
    17221 gcctgttccg gaaattcccg cgcccagtag agataatgct cacgaggata atggcacagc
    17281 gctctgtctg gtccgccgtg aacctttttc tccgcctgct cgtcaccttc cagccccagc
    17341 tccgttagca tcaactcacc atcaacctgg attttggcaa ttgcgctggg tttgccttcg
    17401 ggataagcct gaatcttgcc tgtgtataca tcaaccggat atcgcatctg ttccctctcc
    17461 acgagaaatt tccccattag accatattta ttttatgtca ggcgtgctgt tttcgcggta
    17521 agtatccagg caggaactca atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg
    17581 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgccg ctctggcttt
    17641 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgactgc
    17701 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
    17761 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
    17821 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
    17881 tactgccaca cccaacagga tccccagcaa acctgtctgg gcaattacag tcaaatcaat
    17941 ggtattgcct agtgcgaagg caaagaatgg aatcagcgtc tgcaccgctt tgctgaaaaa
    18001 ttctcgtaat tcagggtcaa ggttcccaag ggcaaagccc accaggaacg gtaatactgc
    18061 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
    18121 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt cttttgtgcc
    18181 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
    18241 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
    18301 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
    18361 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
    18421 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
    18481 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
    18541 cccccccggg attttctcaa tcaagcgttt tatctgcatt tccatcacct gtcggcatat
    18601 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
    18661 acattcattt aatcaataaa agtgatcaaa accacatcaa ctgaaacgcc gttttatttt
    18721 tatcggatca ttgtttttat tttgaaattg atcacaaaaa aaccaccgcc attggcgatg
    18781 gttcattttt taagctgata tgcggcgtaa acgcctcact gcagcagacg gcaaatgatt
    18841 attttttcgc cgcgaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
    18901 tgatgtagtc cgggcggcgg ttctggaatt tcaggtagta agcatgctcc cacacatcca
    18961 ggcccagaat cgggaagccg gatgcgccag aaatagcttc acccatcagc ggggagtcct
    19021 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
    19081 aaccaaagcg ggaagctgcc gctttctcaa attctgcttt gaagttatct acggaaccga
    19141 aatcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
    19201 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
    19261 ctgctggcag ctggtccagt ttggtgatca actcttcaac cggcaggttg gcaaattctg
    19321 gcaggctttc caacgccgcg ttggcgttgt tgacgtaggt ctgatggtgt ttggtgtggt
    19381 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
    19441 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
    19501 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
    19561 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
    19621 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
    19681 gcacgatttt tacactcatc tcgtcggaga tgtgacgcga cgaaaaatga tgaggataag
    19741 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
    19801 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
    19861 gtcggtggga ttgtttcgtg gattattctg ccgtaggcca tcagcgccct gttactaccg
    19921 aatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
    19981 ggcgctatgt gggggatcgg taatatcaac tacggcctga ccatgcgtta tctcggcatg
    20041 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
    20101 attatcaacg gcaatttcga tgtgttgatt aacaccgaag gcggacgcat gacgttgctc
    20161 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
    20221 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aaggactggt gctggcggtg
    20281 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
    20341 gaagccgctg ccgcactagg cgtcgatcca ctgtatgtcg ctctgccaag ctatgttgtc
    20401 atcatgggcg gcggcgcgat catcaacctc ggtttctgtt ttattcgtct ggcaaaagtg
    20461 aaggatttat cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
    20521 ttactctcag cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctggggc
    20581 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
    20641 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatgc aggacgccgt
    20701 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg ttgccgccaa catcgttggc
    20761 atgggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
    20821 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
    20881 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
    20941 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
    21001 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
    21061 ctagccgcca accgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
    21121 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactct
    21181 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
    21241 tgccgcgcct gtgccatccc catgctacct aagcgccagt gtggttgccc tgcgctggcg
    21301 ctaaatcccg gaatcgcccc ctgccagtca agattcagct tcagccgctc cgggcaataa
    21361 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
    21421 atgtaaaaga gatcgccacg ggtaatgcga taagggcgat cgttgagtac atgcagacca
    21481 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcagc aaagacatct
    21541 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
    21601 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaaa ttgattattc
    21661 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
    21721 ctgacgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
    21781 actgtcgctg aatccgcaat gataggtgat gtcagtaacg ctggcctcgc tgtgacgtag
    21841 cagatgtcgg gctttcatca gtcgcaggcg attcaggtat cgctgaggcg tcagtcccgt
    21901 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
    21961 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
    22021 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
    22081 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
    22141 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
    22201 cggatactgc ccatccagct cttgtggcag caactgattc agtccggcga gaaactgaaa
    22261 tcgatccggc gagcgataca gcacattagt cagacacaga ttatcggtat gttcatacag
    22321 atgccgatca tggtcgcgca caaaacagac cgtgccaccg gtgatggtat agggctgccc
    22381 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
    22441 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttaccaga
    22501 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
    22561 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
    22621 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
    22681 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
    22741 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
    22801 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
    22861 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
    22921 aaatccatcg ttttaacaat gggctgcata gtcagaacgg ctatgtcacc tgggatgtgg
    22981 atagccttga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
    23041 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
    23101 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggccta atggcgcagg
    23161 cacaacaaca actcggcaaa cgcgatattt atcaacgtag cggcatccag tttctgccct
    23221 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
    23281 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
    23341 gggaatatac caacgccacg accacgcaac tggtcaatat caatagcgac gactgggacg
    23401 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
    23461 gtaatgtcat aggtcactgg atttgcccgc agggtaatga gattccagtg gtcgccgttg
    23521 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgtgctgctt
    23581 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
    23641 acacggcact ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
    23701 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggagcgg caaatcaacg
    23761 atcttccggc gcttatctcc gcgacacagg cacttccggc ttgccgcttc attatcaatc
    23821 ccaatgacga tcgctttatt aatcctgaga cgatgtgcag cgaaattcag gctgcgtgtc
    23881 gggaaacggc gcaaccgatc ccggaaagtg atgctgaact ggcgcgctgc attttcgaca
    23941 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
    24001 tctcgcaact gcatattgtc ggcggaggct gccagaacac gctgctcaac cagctatgcg
    24061 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcgacg ctcggcaata
    24121 tcggcatcca gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
    24181 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
    24241 atgtggcgcg gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
    24301 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggca gtagggattg atgtcgagga
    24361 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gtgatgatgt
    24421 ttccggtttt gaaaacccgg aaggttcgct gaccggaggg attcaggcta ctggcaatta
    24481 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
    24541 gattccgggg ccaaaacggc ttaatttaca tgccatctat ctggaatcag acacgccagt
    24601 ctcgcgcgac cagatcaaac ctgagcactt caaaaactgg gttgaatggg cgaaagccaa
    24661 tcagctcggt ctggatttta acccctcctg tttctcgcat ccgctaagcg ccgatggctt
    24721 tacgctttcc catgccgacg acagcattcg ccagttctgg attgatcact gcaaggccag
    24781 ccgccgcgtt tcggcctatt ttggtgagca actcggcaca ccgtcggtga tgaacatctg
    24841 gatcccggat ggcatgaaag atatcaccgt tgaccgtctc gctccgcgcc agcgtctgct
    24901 ggcagctctg gatgaggtga tcagcgagaa gctggatccg gcgcaccata tcgacgccgt
    24961 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
    25021 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc atttccaccc
    25081 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtgccgc agttgctgct
    25141 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
    25201 ccaggcgatt gccagtgaga ttgttcgtca cgatctgttt gaccgggtgc atatcggcct
    25261 cgacttcttt gatgcctcta tcaaccgtat tgctgcgtgg atcattggta cacgcaatat
    25321 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
    25381 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgtggca
    25441 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
    25501 gagcgtgcgg gcatatgaga aagaaacttt gagtcgccgc gggtaaacac tgccggatgc
    25561 ggcacaagtg ccttatccgg cctacaggcc ggcaatagtt gtaggcctga taagacgcga
    25621 cagcgtcgca tcaggcattg attgccggat gcggcacgag cgccttatcc ggcctacggg
    25681 tcgtgcatct gacaacaccg aatttacagg aacacagaac atgcaaaaca ttacccagtc
    25741 ctggtttgtc cagggaatga tcaaagccac caccgatgcc tggctgaaag gctgggacga
    25801 gcgcaacggc ggcaacctga cgctgcgcct ggatgacgcc gatatcgcgc tttatcacga
    25861 caacttccac ccacaaccgc gctatattcc gctcagccaa cccatgcctt tactggcaaa
    25921 tacgccgttt attgtcaccg gctccggcaa attcttccgt aacgtccagc tcgatcctgc
    25981 agctaactta ggcgtcgtaa aagtcgacag cgatggcgcg ggctaccata ttctctgggg
    26041 gttaaccaac gaagccgttc caacttctga acttccggct cactttctct cccactgcga
    26101 gcgcattaaa gccaccaacg gtaaagatcg ggtgatcatg cactgccacg ccaccaacct
    26161 gatcgccctc acctatgtac ttgaaaacga caccgcggtc ttcactcgcc aactgtggga
    26221 aggcagcacc gagtgtctgg tggtattccc ggatggcgtt ggcattctgc cgtggatggt
    26281 acccggcacg gacgaaatcg gccaggcaac tgcccaagag atgcaaaaac attcgctggt
    26341 gttgtggccc ttccacggtg tgttcggttc cggaccgacg ctggatgaaa ccttcggttt
    26401 aatcgacacc gcagaaaaat cagcggaagt gttagtgaag gtgtattcga tgggcggcat
    26461 gaaacagact atcagccgtg aagagttgat agcgctcggc cagcgtttcg gcgttacacc
    26521 actcgccagt gcgctggcgc tgtaaggagc aaacatgatc cgcaaagcct ttgtcatgca
    26581 ggtaaacccc gacgcccacg aagagtatca gcgtcggcat aatcccatct ggccggaact
    26641 ggaagcagtg ttgaaatctc acggtgcgca taactacgcc atttacctcg acaaagcgcg
    26701 taatctgctg tttgccacgg tagagattga atctgaagaa cggtggaatg cggtagccag
    26761 cactgaaatc tgccaacgtt ggtggaaata tatgaccgat gttatgcccg ctaacccgga
    26821 taacagcccg gtgagtagcg agctgcaaga agtgttttac ctgccttaat gagttgcggt
    26881 gccggaaaat atccagcacc gcgacttcat cagaagaagt atttaaagcg aacgcgtgcg
    26941 ccgtaacggt catcagtatc atcaccgtgt tttttatcgc cgtcgctatc cagaatggag
    27001 taatacgtcc cgaggtaaat attaaagttc tccatatcca tcacgttagc gaactgataa
    27061 gacgcatgaa tggtgtgaat ggtgtacgtc ccttcatcat caatgcagca gtcgttatca
    27121 caaactacac cgctgaattc atcaatctta ttgtgcgcgt agatataacc gagctcgaaa
    27181 cgtttccaca gcgcgttaat cccggcagtg aaatctttct cattattagc atctaaatag
    27241 gcggtattta gattaaccac aatgccattg tccggatccg ttttcaggcc gttccaggtc
    27301 atgctcatac catagccggt acgatcggac tgatcgacaa aattaccctt gctatcggta
    27361 taaccataag cattatttac cacgttcgct tccatcgctg cggaaacggt gaattcttcc
    27421 gtcggcgacc aggcaattac tgggcgcaga taagcaacat ttttctgctg ctccatatcg
    27481 cgtccatgat aattaccgtc attatataaa gatgttccgt cttcaagtaa cgtgtttaat
    27541 tcaaaatacc agttatcgag ttgtttgctg acgaggaaat taccgccagc gttagagcgc
    27601 ccgcggccct ctttcatcat atagatataa ccgctgccat cgtcataaag atcgttcgca
    27661 gtattaccgg aatgttcaac aaaggtatcc tgattcaatg ggaacatatc gtaggcttca
    27721 aaacggccta ctttgacctt ccagtcgttt tctttgccaa agaagaatac cgcatcatcg
    27781 atatttactg aaccgtgcat atcacccagc ggttgtgcgg agaacccggc gaaataaccg
    27841 ttgtccatct ttcgcatacc gtcaaaacct aacagaatac gaccattgag attccactgt
    27901 tcattagtgg gatcgctatt cacatcagcg ttggtcattg ccagcaggcc atgattactt
    27961 tccgcatcca tattaaattc tacgtcgccg tagattttta aatcgccata acctgaaagc
    28021 aaaagattag gtgaggtggc atttttcttt gcagattccg ccgttgcctg tccatttacc
    28081 ggcgtattgc cctgagcggc cttaatttcg cggatctcag cggcttgttg tgtctgcagc
    28141 tgtttaacct ggctctctgc ttttgccgcc cgcatttctg ccgcttccag gcgttcttcc
    28201 agcaatgcca tgcgttgttc cagcattaaa tgctgtgttt ttgccatacc ggtactacat
    28261 gccaataata aaccgatggc taaagataat gtcgattttt tcattattta cgttccctga
    28321 aataaaggag accctacgct ttccaaaagg aatacgtata tagagaaata taaggtgtgc
    28381 actttcgaat ttttaatcca acttcagcag gccgatggat agtatcccgc cgcataatat
    28441 gagtaaatcc gctgatttcc tggaacgtga ggaatataac aatagtgccg tttttaacta
    28501 aatatcgtaa aaaatattag atatgattca agctggctca taagccacaa atataaataa
    28561 cgtttagaca aattatctga gacatttaaa cattcacaaa atcattaaat aataactctt
    28621 tattcacaag atttatttta cctactaata tgtgccttac gccgcaaaat ttatccttca
    28681 ctctgcttta tattgtcacg acttcacgac atatccgcgc tcatcacaaa cccgccccgc
    28741 tattacaatt aactgaaata ttttttcatg gagtccgatt aatggcagct cttactgcaa
    28801 gctgtattga cctgaatatt cagggcaatg gcgcttattc cgttctgaag cagttggcga
    28861 caatggcgtt acaaaacggt tttatcaccg actcacacca gttcctgcaa accctgctgc
    28921 tgcgcgaaaa aatgcactcc accggttttg gttctggtgt cgccgtgccg cacggtaaaa
    28981 gcacctgcgt taaacaaccg ttcgtgttat tcgcccgcaa agcgcaggct attgactgga
    29041 aagccagcga cggcgaagac gtcaattgct ggatctgcct cggcgtaccg caaagtggcg
    29101 aagaggatca ggtcaaaatc atcggcacac tgtgtcgcaa aattattcac caggaattta
    29161 ttcatcaact gcaacagggc gataccgacc aggtgcttgc cttgttaaat caaaccctca
    29221 gctcataagg aagtggcgat ggagtcatcc ttacgtattg tcgcgatcac caactgcccc
    29281 gccgggatcg ctcacaccta catggtggcg gaagccctgg aacagaaagc ccgttctctc
    29341 ggccatacca ttaaagtgga aactcaaggg tccagcggcg ttgaaaaccg cttatccagc
    29401 gaagagattg ccgctgccga ttacgtgatt ctcgccaccg ggcgtggcct gagcggtgac
    29461 gatcgcgcac ggtttgccgg gaaaaaagtt tatgagattg ccatctccca ggcgctgaaa
    29521 aatatcgatc agattttcag tgaattaccg acaaattcgc agctttttgc cgcagatagc
    29581 ggcgtgaagc taggcaaaca ggaagtacaa agcggcagcg tgatgagcca cctgatggcg
    29641 ggcgtctctg cggcgctgcc gtttgtcatc ggcggtggta tcctggtggc gcttgccaac
    29701 atgctggtgc agttcggttt gccgtatacc gatatgtcga aaggcgcgcc gtcgtttacc
    29761 tgggtagttg aatccatcgg ttaccttggc ttcaccttta tgatccccat catgggcgct
    29821 tatattgcct cgtcgattgc cgataaaccg gctttcgctc cggcgtttct ggtttgctat
    29881 ctggcgaacg acaaagcgct gctcggcacc cagtcgggtg cgggattcct cggcgcggtg
    29941 gtgctggggc tggcgattgg ctatttcgtc ttctggttcc gtaaagtccg tcttggcaaa
    30001 gcattacaac cgctgctcgg ctccatgctt atcccattcg ttactctgct ggtttttggc
    30061 gtgctcactt actacgttat cgggccggtg atgtccgacc tcatgggcgg gctactgcac
    30121 ttcctgaata ccattcctcc atcaatgaag tttgcggcag cgtttctggt cggtgcgatg
    30181 ctggcgttcg atatgggcgg ccccatcaat aaaaccgcct ggttcttctg cttctcactg
    30241 ctggaaaaac acatttatga ctggtacgcc atcgtcggcg ttgtccccgt cgcggcgggt
    30301 cttgcgacgt tcattgcgcc caaactgttt acccgacagg aaaaagaggc tgccagtagt
    30361 gccattgtgg tcggtgccac agtcgcaacc gaaccggcta ttccttacgc tctggcagct
    30421 ccgttgccga tgattaccgc caatacgctg gcgggcggca ttaccggcgt gctggtgatc
    30481 gctttcggaa tcaaacgtct ggcacctggt ttgggtatct ttgacccgct gatcggcctg
    30541 atgtcgccgg taggttcgtt ttatctggtg ctggcgattg gcctcgcgct gaacatttca
    30601 ttcattatcg tcctgaaagg attgtggtta cgccgcaaag cgaaagccgc gcaacaggag
    30661 cttgtccatg aacattgagt tactgcaaca gttgtgcgaa gccagcgccg tcagcggcga
    30721 tgaacaggaa gttcgcgaca ttctgataaa cacgctggaa ccttgcgtta atgagatcac
    30781 ctttgatggt ctgggcagct ttgttgcccg taagggaaat aaaggtccaa aagttgccgt
    30841 tgtcgggcat atggatgaag tcggctttat ggtcacccac atcgacgaga gcggttttct
    30901 gcgctttacc accattggcg gctggtggaa tcagtcgatg ctcaaccacc gggtaacaat
    30961 acgcacacac aagggagtga aaatccctgg tgtgattggt tccgtagcgc ctcatgcgtt
    31021 aacggaaaag caaaagcaac aaccgctgtc atttgatgag atgttcattg atattggcgc
    31081 gaacagtcgc gaagaagcga aaaaacgcgg tgcggaaatt ggcgatttta ttagcccgga
    31141 agccaatttt gcctgctggg gcgaagataa aatagtcggc aaggcgctgg ataatcgcat
    31201 cggctgcgcg atgatggctg agctactaca gacagtaaat aacccaggaa ttacgctgta
    31261 cggcgtcggc agcgtggaag aagaagttgg gctacgcggg gcacaaacct cggctgaaca
    31321 cactaaaccg gatgtggtga tcgtgctgga taccgccgtc gcgggcgatg ttccgggcat
    31381 tgataacatt aaatacccgc tgaaactggg caacgggccg gggctgatgc tgtttgacaa
    31441 gcgctactta cccaaccaga aactggtggc ggcgttaaaa aactgtgccg cacataacgg
    31501 tttaccgctg caattttcca ccatgaaaac cggagcgacg gatggcgggc gctacaacgt
    31561 aatgggcgga gggcgtccgg ttgtcgcgct gtgtctgcca actcgttatc tgcacgctaa
    31621 cagcggtatg atttcaaaag ccgattatga tgctctgctc acgctgatac gggattttct
    31681 gacgacctta actgcggaga aagtcaacgc gtttagccag ttccgtcagg tggattaatg
    31741 ctaaacgaac gccagttaaa gattgtcgat ctgctggagc aacagccgcg cacgcctggc
    31801 gagctggcgc aacagactgg cgtttcaggc aggaccatcc tgcgtgatat tgactatctc
    31861 aactttaccc ttaacggcaa agctcgtatc tctgccagtg gcagtgcggg ctatcagttg
    31921 gaaatcttcg agcgccgcag cttttttcag ttgctgcaaa agcacgataa cgacgatcgg
    31981 ctgctggcgc tgttattact gaataccttt actccccgtg cgcaactcgc ctcggcgctt
    32041 aatttgccag aaacttgggt cgcagagcgt ctgccccggt taaaacagcg ttatgaacgc
    32101 acttgttgcc tggccagtcg tcccggtttg ggccatttca ttgatgaaac agaagagaaa
    32161 cgcgttatct tgctggcgaa cttgctacgc aaagatccgt ttttaatacc tttggctggc
    32221 gtaacacgag acaaccttca gcatttatcc acggcctgcg acaaccaaca ccgctggccg
    32281 ctcatgcagg gggattatct ctccagcctg attctggcga tttacgccct gcgtaatcaa
    32341 ctgaccgatg agtggccgca atatcccggt gacgagataa aacaaatcgt tgaacataac
    32401 ggtctgtttc ttggtgataa cgctgtaaga accctgacag gtttgataga gaaacagcat
    32461 cagcaagcgc aggtaatttc tgccgatcat gtgctggggt tgctgcaaag ggtgccaggt
    32521 ctcgcgtcat tgaatattat tgatacgcag ctggttgaga acattaccgg tcatttatta
    32581 cgttgccttg ccgcaccagt gtggattgct gagcatcgcc aaagcagcat gaataacctg
    32641 aaagccgcct ggcctgcggc ctttgatatg agtctgcact ttattacgct actgcgtgaa
    32701 cagctcgata ttcccctttt cgacagcgat ctgatcggtt tgtattttgc ctgtgcgctg
    32761 gaacgacatc aaaacgaacg ccagccgatc attttgctct cggaccagaa cgcgattgcc
    32821 actattaatc agctcgccat tgagcgcgat gttttacatt gtcgggtgat tattgcccgt
    32881 agcttaagcg aacttgttgc cattcgcgaa gagattgagc cgttattgat cattaacaac
    32941 agccattatt tactggatga cgcggtaaat aattacatca ccgtaaaaaa tatcattacg
    33001 gctgccggta tcgaacaaat aaaacatttt ctggcgacgg catttattcg ccagcaaccg
    33061 gagcgttttt tctctgcccc cggaagtttt cattattcga atgtacgcgg tgaaagctgg
    33121 caacatatta cccggcaaat ttgtgcgcaa ttagtcgcac aacaccatat taccgccgat
    33181 gaagcgcaac ggatcatcgc ccgcgaaggc gaaggtgaaa acctgattgt taatcgcctc
    33241 gccatcccac attgctggag cgaacaggag cgacgttttc gtggattttt tattaccctc
    33301 gcccaaccag ttgaggtgaa taacgaagtc attaaccatg tcttgatcgc ctgcgccgcc
    33361 gccgatgcgc gtcatgagct aaaaatattt agctatctgg caagcgtatt gtgtcagcat
    33421 ccggcagaag ttattgccgg gctaacagga tatgaggcat ttatggagtt acttcacaag
    33481 gggtgaactg tgtcggatgc gccgcatccg acactgcatt tatcatcaaa aacgtttaac
    33541 gcaggctatt atagtcatca accatttcgt tgtactcgcg taacgctcgt ggaaatgagt
    33601 cttcgaccat ccagcttgtg tttgcatcct gaagatgctc tttatcccag tcagagtacg
    33661 ggactttatc tctgacgcgg cgaacatatt ttttagtctc gagttgatac tggtctgccg
    33721 aattaatgaa cgagaaattc atgccgcctt tatccgcttc tttggcctgc gccaccagcg
    33781 tttccagttc agacactttc ttcattgccg catcaacatc aaacttctct tgcgatatca
    33841 ggttattaat ttgtttcgct gaaatcatga ctgccagaga atagtagtgg aatgttttcc
    33901 cttctctttc ttcaatattt ttcagttccg caagctggcg cttatcatta atttcctgaa
    33961 tcgccgcatg ataagattct gctacaggtt caaaggcttc cagatttttt aagaatgtct
    34021 ggtgcagcgt tttccctttc gcaaacgcat cgtctttata attttcctgg gtgtaatatt
    34081 tatccatttc gttaatggta ttacccaacg ccacggcagc atcgatataa ctcaccgcca
    34141 cgccatcaat tggttgcagc gcaggcgtta acgccacagc actttttacg cctttttcac
    34201 actcagcgag gttggattca ctaatgccgt agatgccata aacagtactc tctttaccgg
    34261 taggcccctg tttaaaatct ttcagccagt cagcataacg cgccagactg cgctgtaccg
    34321 ggatctgtaa cttgttgtag cacttgatat agacatccat tttttcgtcg aaaaccgctt
    34381 gttcatcgac cagcggttgc gcaggtgttt caggtttagc atctgctttt gcaacgggag
    34441 cttcagtagg tttcgcttca ggagctggtg cggcaggttg actattggca ggcaggggcg
    34501 tttctgtttc ggctttttta tcatcacaac ccgtcagacc gagggccatg atggcgacta
    34561 taatagcgga agataataaa ttccttttca
//
LOCUS       DAZFDC010000036        34261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFDC010000036 DAZFDC010000000
VERSION     DAZFDC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.036"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(128..803)
                     /locus_tag="ACHJ48_001878"
                     /pseudo
     CDS             complement(128..803)
                     /locus_tag="ACHJ48_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(948..1556)
                     /gene="yiiX"
                     /locus_tag="ACHJ48_001879"
     CDS             complement(948..1556)
                     /gene="yiiX"
                     /locus_tag="ACHJ48_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO4707444.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1616..1933)
                     /gene="metJ"
                     /locus_tag="ACHJ48_001880"
     CDS             complement(1616..1933)
                     /gene="metJ"
                     /locus_tag="ACHJ48_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO4707445.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2210..3370
                     /gene="metB"
                     /locus_tag="ACHJ48_001881"
     CDS             2210..3370
                     /gene="metB"
                     /locus_tag="ACHJ48_001881"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO4707446.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAKAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3373..5805
                     /gene="metL"
                     /locus_tag="ACHJ48_001882"
     CDS             3373..5805
                     /gene="metL"
                     /locus_tag="ACHJ48_001882"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO4707447.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEVSELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPTSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQRADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(5944..6078)
                     /locus_tag="ACHJ48_001883"
     CDS             complement(5944..6078)
                     /locus_tag="ACHJ48_001883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707448.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6154..7044
                     /gene="metF"
                     /locus_tag="ACHJ48_001884"
     CDS             6154..7044
                     /gene="metF"
                     /locus_tag="ACHJ48_001884"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO4707449.1"
                     /translation="MNFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7373..9553
                     /gene="katG"
                     /locus_tag="ACHJ48_001885"
     CDS             7373..9553
                     /gene="katG"
                     /locus_tag="ACHJ48_001885"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO4707450.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9647..10552
                     /gene="yijE"
                     /locus_tag="ACHJ48_001886"
     CDS             9647..10552
                     /gene="yijE"
                     /locus_tag="ACHJ48_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO4707451.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10579..11196)
                     /gene="yijF"
                     /locus_tag="ACHJ48_001887"
     CDS             complement(10579..11196)
                     /gene="yijF"
                     /locus_tag="ACHJ48_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO4707452.1"
                     /translation="MKASLALVSLLTAFTSYSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11470..12573)
                     /gene="gldA"
                     /locus_tag="ACHJ48_001888"
     CDS             complement(11470..12573)
                     /gene="gldA"
                     /locus_tag="ACHJ48_001888"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO4707453.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12584..13246)
                     /gene="fsa"
                     /locus_tag="ACHJ48_001889"
     CDS             complement(12584..13246)
                     /gene="fsa"
                     /locus_tag="ACHJ48_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO4707454.1"
                     /translation="MELYLDTANVAEVERLAHIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13258..15759)
                     /gene="ptsP"
                     /locus_tag="ACHJ48_001890"
     CDS             complement(13258..15759)
                     /gene="ptsP"
                     /locus_tag="ACHJ48_001890"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4707455.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16068..17147
                     /gene="frwC"
                     /locus_tag="ACHJ48_001891"
     CDS             16068..17147
                     /gene="frwC"
                     /locus_tag="ACHJ48_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO4707456.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17162..17482
                     /gene="frwB"
                     /locus_tag="ACHJ48_001892"
     CDS             17162..17482
                     /gene="frwB"
                     /locus_tag="ACHJ48_001892"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4707457.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17533..19830
                     /gene="pflD"
                     /locus_tag="ACHJ48_001893"
     CDS             17533..19830
                     /gene="pflD"
                     /locus_tag="ACHJ48_001893"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO4707458.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19796..20674
                     /gene="pflC"
                     /locus_tag="ACHJ48_001894"
     CDS             19796..20674
                     /gene="pflC"
                     /locus_tag="ACHJ48_001894"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO4707459.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20676..21017
                     /gene="frwD"
                     /locus_tag="ACHJ48_001895"
     CDS             20676..21017
                     /gene="frwD"
                     /locus_tag="ACHJ48_001895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO4707460.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQQVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21004..21855)
                     /gene="yijO"
                     /locus_tag="ACHJ48_001896"
     CDS             complement(21004..21855)
                     /gene="yijO"
                     /locus_tag="ACHJ48_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4707461.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22070..23803)
                     /gene="eptC"
                     /locus_tag="ACHJ48_001897"
     CDS             complement(22070..23803)
                     /gene="eptC"
                     /locus_tag="ACHJ48_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4707462.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNMFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELNALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKENTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(23986..26637)
                     /gene="ppc"
                     /locus_tag="ACHJ48_001898"
     CDS             complement(23986..26637)
                     /gene="ppc"
                     /locus_tag="ACHJ48_001898"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO4707463.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(26989..28140)
                     /gene="argE"
                     /locus_tag="ACHJ48_001899"
     CDS             complement(26989..28140)
                     /gene="argE"
                     /locus_tag="ACHJ48_001899"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO4707464.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICAWCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28294..29298
                     /gene="argC"
                     /locus_tag="ACHJ48_001900"
     CDS             28294..29298
                     /gene="argC"
                     /locus_tag="ACHJ48_001900"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO4707465.1"
                     /translation="MLNTLIVGASGYAGAELVTCVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLDAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29306..30082
                     /gene="argB"
                     /locus_tag="ACHJ48_001901"
     CDS             29306..30082
                     /gene="argB"
                     /locus_tag="ACHJ48_001901"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO4707466.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30143..31516
                     /gene="argH"
                     /locus_tag="ACHJ48_001902"
     CDS             30143..31516
                     /gene="argH"
                     /locus_tag="ACHJ48_001902"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO4707467.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLTELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            31783..32700
                     /gene="oxyR"
                     /locus_tag="ACHJ48_001903"
     CDS             31783..32700
                     /gene="oxyR"
                     /locus_tag="ACHJ48_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO4707468.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32683..34083)
                     /gene="sthA"
                     /locus_tag="ACHJ48_001904"
     CDS             complement(32683..34083)
                     /gene="sthA"
                     /locus_tag="ACHJ48_001904"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO4707469.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         7750 a         8646 c         9561 g         8304 t
ORIGIN      
        1 ataatatatg tcatttgaga aaaaagagca gaatgaggta aaaatctttg gctgtgtatt
       61 cagtctggta atcatccaag gaaagctcat cagaagggtg atgactccaa ttagtagaac
      121 atatattttc gataaaagca ccgatgactt tttcatgaag ttctacagaa ctcgagaaac
      181 agatggttct gcgggccaga cgtttgattc gggttctcag ggtcagattg ttacgctcaa
      241 tgtgctgagt aaaaatctta ccagtcagat gcttctcctt tggtaactct ctagcgtaac
      301 taccccattc gtcagtggtc aacatgccga tggtaaaagg gtaagcagcg ccagcaattc
      361 acgacaggtt tcatcagtcc gggggccaaa agtgtaggcc agaacaccac cagttttcgt
      421 gttgtacacg taccagagcc agtgctgtcg cgctttactg ccgacaaagc tccattgctg
      481 actaagtttg cagatgagtg ccacatcagc atgagcgacc ggggatgacg ttattcttcg
      541 tggcgagagt tttttaaagt tcggatgact gtattaatgc cgattttcag cgtacgggca
      601 gtatagcgaa ccccggcacc gttgaaagcc atttcagcga tttggtcttt aactcccggt
      661 ttacggggct cgtaacagta actgagctga aatacccggt gacagtcacg gccgcggaat
      721 cgatcatgtc ttttggggtt ctgcccgcga cggtaaacct gaacggactg gcaacgggga
      781 caatgaaggt taactgtgac catgaaacat ccttaaaagc gagtattata tccttaattc
      841 aactaattgg aggcatcacc gtttaccgga gcgacagctt gcggaaaggt cgtgcaacta
      901 caaagcaaca aacagactac ggcgggaatt atttttttca tcatccatta ccacgaaaac
      961 agcggccatt ctttggcaac cgtggtgagt tgtggcgcat cgaaaacggc ttgtggtgag
     1021 accaccgttt cctcaagcgg aatatttttg ccgtagcgtt ctttgagctt cgcctggacc
     1081 agcgggttgc tgagatcaaa ctctttcaac ttctgctgtt cgcccacacg cattcccagc
     1141 gcattctgat aaaccttcca caccacttcc gaacagtact ggcgatcgtc gctccatgag
     1201 aagctgaaat cgtacggttt gccgagataa cgtttcgccg tttgcgccag tttttgctgt
     1261 tgttcaacac tcagtccgcc ttcaacgcgg cgaacaacgt atttgccctt ttcaccatgg
     1321 gcaatccact gcttgagcgg ggtgtatttc accgggccga ccgcttcaaa aacgtagggc
     1381 tttttgttac gcatcaccag cataccggtg tggctgtaat cggaatgggt cgccagttgg
     1441 atcgctttac tttgcgatga gcgagagatt tgaaagatga tgtcgccagt ttgcggctgc
     1501 caggcaaagg caggcacaga aaccagcagg ctgaggatca gtagcctgat tttcatcgtt
     1561 aaacgtcctt gtatgaaaaa agcggctggt taactccgcg ccgctctttt ccgctttagt
     1621 attcccacgt ctccgggtta atccccatct cacgcatgat ctcttttgcc gcttccggga
     1681 tttcgtcgct gcgctcttta cgcagatcgg catcatccgg caaaggttgc ccggtaaagg
     1741 catgcagaaa cgcttcgcac agcagctcgc tgttggtagc gtgacgcagg ttgttcacct
     1801 gacgacgcgt gcgttcatcg gtgaggattt ttaacacctt aagaggaatg gaaaccgtaa
     1861 tctttttgac ttgttcactc ttcttgccgt gctcagcgta tgggctgata tattcgccgc
     1921 tccattcagc catgagatac ttaatcctct tcgtcaataa attgagacca gaccacacaa
     1981 tttatgtggg tactgaccgt aaacccgcat agtttaccgt acaggcgtta ccgtgacatc
     2041 gtgtaatgca cctgtcggcg tgataatgca tataatttta acggctattt gggatttgct
     2101 caatctatac gcaaagaagt ttagatgtcc agatgtattg acgtccatta acacagtgtt
     2161 tactctggtg cctgacattt caccgaccaa gcccagggaa cttcatcaca tgacgcgtaa
     2221 acaggccacc atcgcagtgc gtagcgggtt aaatgacgac gaacagtatg gttgcgttgt
     2281 ccctcccatc catctttcca gcacctataa ctttaccgga tttaatgaac cgcgcgcgca
     2341 tgattactcg cgtcgcggca acccaacgcg cgatgtggtt cagcgtgcgc tggcagaact
     2401 ggaaggcggt gctggtgcag tacttactaa taccggcatg tccgcgattc atctggtaac
     2461 gaccgtcttt ttgaaacctg gcgatctgct ggttgcgccg cacgactgct acggcggtag
     2521 ctatcgcctg ttcgacagcc tggcgaaacg cggttgctat cgcgtgttgt ttgttgatca
     2581 aggcgatgaa caggcattac gggcagcgct ggcagaaaac cccaaactgg tactggtaga
     2641 aagcccaagt aatccattgt tacgcgtcgt ggatattgcg aaaatctgcc atctggcaag
     2701 ggaagtcggg gcggtgagcg tggtggataa caccttctta agcccggcat tacaaaatcc
     2761 gctggcatta ggtgccgatc tggtgttgca ttcatgtacg aaatatctga acggtcactc
     2821 tgacgtggtg gctggcgtgg tgattgctaa agacccggac gttgtcactg aacttgcctg
     2881 gtgggcaaac aatattggcg tgacgggcgg cgcgtttgac agctatctgc tgctacgcgg
     2941 gttgcgaacg ctggtgccgc gtatggagct ggcgcagcgc aacgcgaagg cgattgtgaa
     3001 atatctgcaa acccagccgt tggtgaaaaa actgtatcac ccgtcgctgc cggaaaatca
     3061 ggggcatgaa attgccgcgc gtcagcaaaa aggctttggc gcaatgttga gttttgaact
     3121 ggatggcgat gagcagacgc tgcgccgttt cctgggcggg ctgtcgttat ttacgctggc
     3181 ggaatcatta gggggagtgg aaagtttaat ctctcacgcc gcaaccatga cacatgcagg
     3241 catggcacca gaagcgcgtg ctgccgccgg gatctccgag acgctgctgc gtatctccac
     3301 cggtattgaa gatggcgaag atttaattgc cgacctggaa aatggcttcc gggctgcaaa
     3361 caaggggtaa aaatgagtgt gattgcgcag gcaggggcga aaggtcgtca gctgcataaa
     3421 tttggtggca gtagtctggc tgatgtgaag tgttatttgc gtgtcgcggg cattatggcg
     3481 gagtactctc agcctgacga tatgatggtg gtttccgccg ctggtagcac cactaaccag
     3541 ttgattaact ggttgaaact aagccagacc gatcgtctct ctgcgcatca ggttcaacaa
     3601 acgctgcgtc gctatcagtg cgatctgatt agcggtctgc tacccgccga agaagccgat
     3661 agcctcatta gcgcttttgt cagcgatctt gagcgcctgg cggcgctgct cgacagcggt
     3721 attaacgacg cagtgtatgc ggaagtggtg ggccacggcg aagtgtggtc ggcgcgcctg
     3781 atgtctgcgg tgcttaatca acaagggctg ccagcggcct ggcttgatgc ccgcgagttt
     3841 ttacgcgctg aacgcgctgc gcaaccgcag gttgatgaag ggctttctta cccgttgctg
     3901 caacagctac tggtacaaca tccgggtaaa cgtctggtgg tgaccgggtt tatcagccgt
     3961 aacaacgccg gtgaaacggt gctgctgggg cgtaacggtt ccgactattc cgcgacacaa
     4021 atcggtgcgc tggcgggtgt ttctcgtgta accatctgga gcgacgtcgc cggggtatac
     4081 agtgccgatc cgcgtaaagt gaaagatgcc tgtctgctgc cattgctgcg tctggatgaa
     4141 gtcagcgaac tggcgcgtct ggcggctccc gttcttcacg cccgtacttt acagccagta
     4201 tccggtagcg aaatcgacct gcagctgcgc tgtagctaca cgccggatca aggctccacg
     4261 cgcattgaac gcgtgctggc ctccggtact ggtgcgcgta ttgtcaccag ccacgatgat
     4321 gtctgcttga ttgagtttca ggtgcccacc agccaggatt tcaaactggc gcataaagag
     4381 atcgaccaga tcctgaaacg tgctcaggtg cgcccactgg cggttggcgt gcataacgat
     4441 cgccagttgc tgcaattttg ctacacctca gaagtggccg acagtgcgct gaaaatcctc
     4501 gacgaagcgg gattacctgg cgaactgcgc ctgcgtcagg ggctggcgct ggtggcgatg
     4561 gtcggggcgg gtgtcacccg taacccgctg cattgccacc gcttctggca gcaactgaaa
     4621 ggccagccgg tcgagtttac ctggcagtcc gatgacggca tcagcctggt ggcagtactg
     4681 cgcaccggcc cgaccgaaag cctgattcag ggcctgcatc agtccgtctt ccgcgcagaa
     4741 aaacgcatcg gcctggtatt gttcggcaaa ggcaatatcg gttcccgctg gctggaactg
     4801 ttcgcccgcg agcagagcac gctttcggcg cgtaccggct ttgaatttgt gctggcaggc
     4861 gtggtggaca gccgccgcag tctgttgagt tatgacgggc tggacgccag ccgcgcgtta
     4921 gccttcttca acgatgaagc ggttgagcag gatgaagagt cgttgttcct gtggatgcgc
     4981 gcccatccgt atgatgattt agtggtgctg gacgttaccg ccagccagca gcgggctgac
     5041 cagtatcttg atttcgccag ccacggtttc cacgtcatca gtgccaacaa actggcggga
     5101 gcaagcgaca gcaataaata tcgccagatc cacgacgcct tcgaaaaaac cgggcgtcac
     5161 tggctataca acgccaccgt cggtgcgggc ttgccgatca accataccgt gcgcgatctg
     5221 atcgacagcg gcgatactat tttgtcgatc agtgggatct tctccggcac gctctcctgg
     5281 ctgttcctgc aattcgacgg tagcgtgccg tttaccgagc tggtggatca ggcgtggcag
     5341 cagggcttaa ccgagcctga tccgcgtgac gacctctccg gcaaagacgt aatgcgcaag
     5401 ctggtgattc tggcgcgtga agcgggttac aacattgaac cggaccaggt acgtgtggaa
     5461 tcgctggtgc ctgctcattg cgaaggcggc agcatcgacc atttctttga aaatggcgat
     5521 gaacttaacg agcagatggt gcaacggctg gaagcggccc gcgaaatggg gctggtgctg
     5581 cgctacgtgg cgcgtttcga tgccaacggc aaagcgcgtg tgggcgtgga ggcggtgcgt
     5641 gaagaccatc cgctggcatc actgctaccg tgcgataacg tctttgccat cgaaagccgc
     5701 tggtatcgcg acaacccgct ggtgatccgt gggcctggcg cggggcgcga cgtcaccgcc
     5761 ggggcgattc agtcggatat caaccggctg gcacagttgt tgtaatttca gaaatttaat
     5821 aatgcccggt actcatgttt tcgggtttat ggtttctaat gaaatatatt gaattatcat
     5881 aggattaggc cggatgaagc gtttacgacg aatccggcaa gaagcaataa gtacatggtt
     5941 agtttatatt tgcagtccgg tttgctttgc ataccggatt ttctttttct taccatcctg
     6001 aagttttttc atcttccctg atttttcctc accatcattg gtcatttttc ggttgacgcc
     6061 cttcggcttt tccttcatct ttacatctgg acgtctaaac ggatggatgt gcacaacaca
     6121 acatataact acaagcgatt gatgaggtaa ggtatgaact tttttcacgc cagccagcgg
     6181 gatgccctga atcagagcct ggcagaagtc caggggcaga ttaacgtttc gttcgagttt
     6241 ttcccgccgc gtaccagtga aatggagcag accctgtgga actccatcga tcgccttagc
     6301 agcctgaaac cgaagtttgt atcggtgacc tatggcgcga actccggcga gcgcgaccgt
     6361 acgcacagca ttattaaagg cattaaagat cgcactggtc tggaagcggc accgcatctt
     6421 acttgcattg atgcgacgcc cgacgagttg cgcaccattg cacgcgacta ctggaataac
     6481 ggtattcgtc atatcgtggc gctgcgtggc gatctgccgc cgggaagtgg taagccagaa
     6541 atgtatgctt ctgacctggt gacgctatta aaagaagtgg cagatttcga tatctccgtg
     6601 gcggcgtatc cggaagttca cccggaagca aaaagcgccc aggcggattt gcttaacctg
     6661 aaacgcaaag tcgatgcagg agccaaccgc gcgattactc agttcttctt tgatgtcgaa
     6721 agctacctgc gttttcgtga ccgctgtgtg tcggcgggca ttgatgtgga aatcattccg
     6781 ggcatattgc cggtatctaa ctttaaacag gcgaagaaat ttgccgacat gaccaacgtg
     6841 cgtattccgg cgtggatggc gcaaatgttt gacgggctgg atgacgatgc cgaaacccgc
     6901 aagctggtgg gcgcgaatat tgccatggat atggtgaaga ttttaagccg tgaaggagtg
     6961 aaagatttcc acttctatac gcttaaccgc gctgaaatga gttacgcgat ttgccatacg
     7021 ctgggggttc gacctggttt ataaatagtg tggcttttat gaaaatcaca cagtgatcac
     7081 aaattttaaa cagagcacaa aatgctgcct cgaaatgagg gcgggaaaat aaggttatca
     7141 gccttgtttt ctccctcatt acttgaagga tatgaagcta aaaccctttt ttataaagca
     7201 tttgtccgaa ttcggacata atcaaaaaag cttaattacg atcaatttga tctacatctc
     7261 tttaaccaac aatatgtaag atctcaacta tcgcatccgt ggattaattc aattataact
     7321 tctctctaac gctgtgtatc gtaacggtaa cactgtagag gggagcacat tgatgagcac
     7381 gtcagacgat atccataaca cgacagccac tggcaaatgc ccgttccatc agggcggtca
     7441 cgaccagagc gcgggggcgg gcacaaccac tcgcgactgg tggccaaatc aactccgtgt
     7501 tgacctgtta aaccaacatt ctaatcgttc taacccgctg ggtgaggact ttgactaccg
     7561 caaagaattc agcaaattag attactacgg cctgaaaaaa gatctgaaag ccctgctgac
     7621 agaatctcag ccgtggtggc cagccgactg gggcagctac gccggtctgt ttattcgtat
     7681 ggcctggcac ggcgcgggga cttaccgatc aatcgatgga cgcggtggcg cgggtcgtgg
     7741 tcagcaacgt tttgcaccgc tgaactcctg gccggataac gtaagcctcg ataaagcgcg
     7801 tcgcttgttg tggccaatca aacagaaata tggtcagaaa atctcctggg ccgacctgtt
     7861 tatcctcgcg ggtaacgtgg cgctggaaaa ctccggcttc cgtaccttcg gttttggtgc
     7921 cggtcgtgaa gacgtctggg aaccggatct ggatgttaac tggggtgatg aaaaagcctg
     7981 gctgactcac cgtcatccgg aagcgctggc gaaagcaccg ctgggtgcaa ccgagatggg
     8041 tctgatttac gttaacccgg aaggcccgga tcacagcggc gaaccgcttt ctgcggcagc
     8101 agctatccgc gcgaccttcg gcaacatggg tatgaacgac gaagaaaccg tggcgctgat
     8161 tgcgggtggt catacgctgg gtaaaaccca cggtgctggt ccgacatcaa atgtaggtcc
     8221 tgatccagaa gctgcgccga ttgaagaaca aggtttaggt tgggcgagca cttacggcag
     8281 cggcgttggc gcagatgcca ttacctctgg tctggaagtg gtctggaccc agacgccgac
     8341 ccagtggagc aactatttct tcgagaacct gttcaagtat gagtgggtac agacccgcag
     8401 tccggctggc gcaatccagt tcgaagcggt agacgcaccg gaaattatcc cggatccgtt
     8461 cgatccgtcg aagaaacgta aaccgacgat gctggtgacc gacctgacgc tgcgttttga
     8521 tcctgagttc gagaagatct ctcgtcgttt cctcaacgat ccgcaggcgt tcaacgaagc
     8581 ctttgcccgt gcctggttca aactgacgca cagggatatg gggccgaaat ctcgttacat
     8641 cgggccggaa gtaccgaaag aagatctgat ctggcaagat ccgctgccgc agccgatcta
     8701 caacccgacc gagcaggaca ttatcgatct gaaattcgcg attgcggatt ctggtctgtc
     8761 tgttagtgag ctggtatcgg tggcctgggc ttctgcttct accttccgtg gtggcgacaa
     8821 acgcggtggt gccaacggtg cgcgtctggc attaatgccg cagcgcgact gggatgtgaa
     8881 cgccgcagcc gttcgtgctc tgcctgttct ggagaaaatt cagaaagagt ctggtaaagc
     8941 ctcgctggcg gatatcatcg tgctggcagg tgtggttggc gttgagaaag ccgcaagcgc
     9001 cgctggtttg agcattcatg taccgtttgc gccgggtcgc gttgatgcgc gtcaggatca
     9061 gactgacatt gagatgttcg aactgctgga gccaattgct gacggtttcc gtaactatcg
     9121 cgctcgtctg gatgtttcca ccaccgagtc actgctgatt gataaagccc agcaactgac
     9181 cctgaccgcg ccggaaatga ccgcgctggt gggcgggatg cgtgtactgg gtgccaactt
     9241 cgatggcagc aaaaacggcg tcttcactga ccgcgttggc gtattgagca atgacttctt
     9301 cgtgaacttg ctggatatgc gttacgagtg gaaagcgacc gacgaatcga aagagctgtt
     9361 cgaaggccgt gaccgcgaaa ccggcgaagt gaaatacact gccagccgtg cggatctggt
     9421 atttggttct aactctgtcc tgcgtgcggt ggctgaagtt tacgccagca gcgatgctca
     9481 cgagaagttt gttaaagact tcgtggcggc atgggtgaaa gtgatgaacc tcgaccgttt
     9541 cgacctgctg taatctgacc ttcttcagcg actgcctttc aggcagtcgc tgaagtttct
     9601 ttaccggcgt atagtgtcca caggaaaact acacactgga tctctcatgt ctgccgcagg
     9661 aaagagcaac ccactggcaa tcagtggcct ggttgtgctc acacttatct ggagttatag
     9721 ctggattttc atgaagcaag tcaccagcta catcggtgcc ttcgacttta ccgccttacg
     9781 ctgcattttc ggcgctctcg ttttattcat cgtcctttta ttacgtggtc gcggaatgcg
     9841 cccgacaccg tttaaataca ccttagccat tgccctgtta caaacctgcg ggatggttgg
     9901 cctggcgcag tgggcgctgg tcagcggcgg agcggggaag gtggcgatcc tgagctatac
     9961 catgccgttc tgggtggtga ttttcgccgc ggtgtttctc ggtgaacgcc tgcgacgtgg
    10021 gcaatatttc gcgattctga ttgccgcttt cggcttattt ttggtgttgc agccgtggca
    10081 actcgatttc tcttcgatga aaagtgccat gctggcaatc ctctccggtg ttagttgggg
    10141 ggcgagcgcg attgttgcta aacgtctgta tgcccgtcat ccgcgcgtgg atttattgtc
    10201 gttaacatcc tggcagatgc tctatgcggc gctggtgatg agtgtggtcg ctttactggt
    10261 gccgcaacgt gaaattgact ggcagcccac tgtgttctgg gctctggcct acagtgcgat
    10321 tctggcgacg gcgttggcgt ggagcttatg gctgtttgtg ctgaaaaact tgcctgccag
    10381 tattgccagc ttaagcacac tggccgttcc cgtttgcggc gtactctttt cctggtggct
    10441 gctcggtgag aatccggggg ccgttgaagg tagcggtatt gtgctgattg tgctggcact
    10501 ggcgctggtg agccgtaaga aaaaagaagc cgttagtgta aaaaggatct gaattttttc
    10561 ttcatgtggg gcgatcgctt atttaacaaa ataacgataa tgccccacca tgcgccagct
    10621 aaacagcaca tcttcttcct gcgcacctgc gccaatgtta tgtatcacca gcggcgtacc
    10681 gtcgcgggcg aagccatctg aaaccacccc aatatgtgcc agcccgttat ctaatcgcca
    10741 ggagacaata tcgcccgctt gatagtcact ggggttcttg ctgatggggc gtgttttatc
    10801 gtggcgggta aaccaggttt ccagattagg cacccgacgg tgatcgatgt tgctgtccgg
    10861 gcgctttaac tgccattttt gcgggtactc ggcaaaattc ttcgccatat cttcgtgcac
    10921 cagtttctgc aaatcgacct tctggctgcg caatgcacgg atcaccacat cggaacatac
    10981 cccgcgttct tgcgggacat caccgccagg ataggtaagc tgcacatacg ccggatcgta
    11041 aaatagcgtg ctgccgattt gctgtctggc accgtctgcg atggcaaggt tggtattggc
    11101 ctggatttgt accacggttg gtggaatggc gggagatttt aaggagtagc tggtaaatgc
    11161 cgttagcagg ctgaccagcg ccagtgaagc tttcatctgt ttcgtccgtg aaaataagag
    11221 tcacagattc gaccttccgg gctaacgatt gttctgtaga gaaaatgtcc agaagcggat
    11281 aaaaaattcc tgaacaacgt gggagtttca gattactggc acagagtgcc ggatgcggcg
    11341 tgaacgtctt atctggccta aaaagcacgc aaattcaata tattgcagag attgcgtagg
    11401 cctgataagc gtagcgcatc aggcaatttt gcgttcaaac tcccggacaa gccgggagtt
    11461 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcgg ctaccagcag
    11521 agcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
    11581 tgcacatgcc gcttctgcca caattcgcat tttcgccggg acatcttctt taatatccag
    11641 ttgagcgaga gttattggca aacctaccgc atggctaagc gcagctacgg tttcgatttc
    11701 ctcaaccggc gagttttcca gtaccagctg cgtcagtgta ccaaatgcca ctttttcacc
    11761 gtgataatag tgatgggcat caggaatggc agtcaggcca ttatgcaccg cgtgcgctgc
    11821 cgccagacca ccgctttcaa agccaacacc gcttaaataa gtgttcgctt caatcacgcg
    11881 ttccagtgcc ggagtcacta catgctgttc ggcggcgagc atcgcttttt cgccttcttc
    11941 cagcaaggtg ttgtagcaca gttcagccag cgccagcgcc gcctgagtgc acttgccgcc
    12001 cgccatggtg gtcgcaccgc tacgagagca ggcacgcgct tcaaaccagg tcgccagcgc
    12061 atcgccgata cccgccgcta acagacgcgc aggtgcgccc gcgacgattt tagtatcgac
    12121 aatcaccata ttcgggttat ttggcagcag cagatagcgg tcaaattcac cttcgtcagt
    12181 gtagataacg gaaagagcgc tacaaggtgc gtcggttgaa gcaatggtcg gggcaatggc
    12241 gaccggaaca cccatgaaat gtgccagtgc tttggctgta tcgagggttt ttccgccacc
    12301 gataccgaga attgcgccac actgcgcagt ctccgcgatg ccacgcagac ggtcgatctc
    12361 attttgcgaa cattcaccgc caaacggcgc aatctctact accagtccag catctttaaa
    12421 gcttttctcg acagtggatt gagcaaaacc taaaacaaat ttgtcaccca ccactaacca
    12481 gcgttctgcc agcggcttca ggtattcgcc cagacgatta atcacatcag cgccctggat
    12541 gtatttaccc ggtgattgaa taatgcggtc cataattgct cctttagaga tgagtagtgc
    12601 caaatgcggc attccagtcg tgttcgaact tctctatagc tgactctacc gcaggggtgt
    12661 tgagcatttg ttgcgctaca tctaagggca gggtgatgga ttcacatcct gccagtaaac
    12721 agtccagcgc ctgacgcggc gttttaaagc tggccgccag caccatgctt tctggtgcgt
    12781 gcatttctaa cagcgcttgc agctcctgaa ccgtacgaat gccgtccccg ccctgggcat
    12841 ccacgcggtt aacatacgga gcaacgtatt ttgcccctgc cagtgcggct aataaccctt
    12901 gtgcggcgct atatacagca gtgccaagtg tagtaatacc ctcttttttc agtattttaa
    12961 ttgctgccag accttcggaa gtcaccggga ttttcaccac aatgcccgga atggcatcgc
    13021 gcagacgctt cgcttcttcc accatcccct gcgcgtcgcg gctcatggtc tgagcaaaca
    13081 gaatgccctc atcaccaatc gctttttgca gacgcggcag cacttcccat atggactcct
    13141 tgctggcagc gataatgctc gggttagttg tcaccccggc aatcgggaat atgtgtgcca
    13201 gacgttcgac ttctgcgacg ttagcggtgt ccagatacag ttccatgatg tttcctttta
    13261 cagttccagt tcatgttgca gcaggctggc gatagcgtcg gcagaggcgg cgttaaccag
    13321 cgcgttacgg aattcttcgt gcatgatgcg gcgagcgagg cgcgagaaaa tgcgcatatg
    13381 ctgatcgccc gcagcgtgtt tgttcagggt taacataatg atgaattgcg cttcatcatc
    13441 gccccagcgc accggcgctt gcagacgcgc tacgctgatg gtggattgct caatgtgttc
    13501 tgatttgctg tggggaatgg caaaactaaa gcccagaccg gtagagaaaa cggcctcgcg
    13561 cgcccacaag tcggcttcca gtttacgcgg atagcggcag cggcccgcca gcagcaggtt
    13621 atcggtcatg cctttgagca cttcttcttt gctgcgccag tcgctttcca gtgtgatgca
    13681 ctcggcggtg accagcggtg cgtcttgttg ggtcatgcgg aattgcgcca gcaggtgttc
    13741 cacttccagc gaagtacggc aggccattgc ctggttgagc aacttgcggc actcacggct
    13801 atcaagttgc gccatccgcg ctttcgccgc cggaattgat ggtgcgctca tgctgagttc
    13861 atccagccct aagccgacca gcaacggcag cacggaacct ttcgctccca gctcaccgca
    13921 cagaccaatc catttgccct ggcgatgcac cgcttgcacg gcgtaatcga gcgcccgcaa
    13981 gaatgccgga ttcaggctgt tgtagtgacg agtaacctta gcgttatcgc gatccactgc
    14041 cagcagatat tgcgtcaggt cattactacc aatactaaag aaatcaatct cttcgcagca
    14101 ttgatcgatg atgaacatca ccgacggcac ttccagcatg atgccgagct ggatcttctc
    14161 atcaaacgga atgtgttcgt tacgcagttg ctgtttggct tccgccagtt tttctttcac
    14221 ccataagatc tcttccattg aggagatcat cgggatcatg attttcaggc tgccgtgagc
    14281 agaggcacgg aggatcgacc gtagttgtgt ggtgaacagc gaggcgtatt cttcataaat
    14341 acgcaccgcg cgataaccga ggaacgggtt agcttctgcc ggaatgttca gataatcaac
    14401 gggtttgtca ccgccaatat ccatagtgcg cacaataatg ctgcgtccgt tggcggactc
    14461 cagcgcttga caaaaaatgt tgtacaactc gctttcgccc ggtgcgctgg tgcgatccat
    14521 atagagcatt tcagtgcgga acaaaccgac gccttccgca ccattgccga atgcggcctg
    14581 cgcttccacg gagtgagcga tgttagcggc aatttcaatg cggataccgt cagcggtacg
    14641 ggcttgttgg gtcagccaga cacgctgttg ctcacgcagg gcgtcctgta cgcgggcttc
    14701 ttgctgataa taacgagcta cggcttcccc tggctcaacc acaatcgccc cggcgttgcc
    14761 gtcgatataa atcgtttgat gctgccacgg agtaagggca tcaatatcta caccaaccag
    14821 tgtcggaatg ttgaacgaac gggcaagaat tacggtatgt gaggtggtgc cgccactttt
    14881 cagtaacaga cctttgaggt gatttttatc cagttcgagg aactggctgg gggttagttc
    14941 atcagccata caaatggcgg gctgcgtcag tttgcccggt gccgggaagc gttgctcacc
    15001 gtagatttgc tgaagcagct ggaagcagac atcgcgtacg tccagggcac gttcttgcag
    15061 gtagctgctg ctggaacggg aaaactcttc acaaaagtga ttcgcactgg cgacaatcgc
    15121 ttcggcgcag cttaatccgg cgctgacgcc tgccagtaaa tgttcgtgca gggaagtatc
    15181 gccagccagg gatcggtgcg cttccagaat cgcgctggtg gcaccgtcgc tatccagcag
    15241 acgaaactca atgtttttca gtaccagcgt caggccgttt tccagtgcgg attgctcggc
    15301 gtcaacgtct ttggctgcgg gaagattacc cagcgcattg agatctaaag aagagatcgg
    15361 cgtcagaatg ccgcctgcgc taccgctgca cacggtgcgg gcgcggataa tttgcggatt
    15421 cagattggtc agtgaaaccg gcagtggttc cagttcgtca gatttaactt ccgccagcgg
    15481 cgcatcgcag tgggggaatt catcgcgcag ccattggctt aaacgctggt gcgcttcctg
    15541 ttcgtcggcc ccggaaatca gtaactggca gttatcgccc gccagcgtgt cggtgccaat
    15601 cagcgcaagg gcgcttttgg cgttgccctt gcggtcagtg cgcaggttat gccactcaat
    15661 ttgtgatgaa aaagtattac acagcgtttc aacgtggctt gccggacgtg catgtacgcc
    15721 gttaggtagc tcacaaataa attccacaat cagggccatt gcctctctcc cggtacggtt
    15781 ttctgtttac aggataagag cgcacggcaa cggcctgcca tgtgacaaat ctgccaaaag
    15841 ctggacaaat gtaatgtaac cgtcaatttg cgacgcgtct cacaagacgc cgttttgcgg
    15901 catgcttccg gattatcgca agttatgagg cggatcgcat ttttgtactg atattacaaa
    15961 agtccagtaa atggcctttt tatccactgt ttgctccgcc tgcgattgcc tattgtttcc
    16021 tccatcaaaa atatggacat gggctttcgc aagcccggga gcagcttatg aatgagttgg
    16081 tgcagatcct gaaaaatacc cgtcagcatt taatgacggg cgtttcacac atgattccct
    16141 tcgtggtatc gggcggtatt ttgctggcgg tttccgtcat gttgtatggc aaaggcgcag
    16201 tgccggatgc cgtagccgat ccgaatctga aaaaactgtt tgatatcggc gttgcaggtt
    16261 tgacgctgat ggtgcctttc ctcgccgctt acatcggtta ctccattgca gagcgttctg
    16321 cgctggctcc gtgcgctatc ggtgcctggg ttggtaacag ctttggtgcg ggcttctttg
    16381 gtgcactgat cgccgggatt atcggcggca tcgtggtgca ttacctgaag aaaattccgg
    16441 tgcataaagt tctgcgctcg gtgatgccca tcttcatcat tccgatcgtc ggcacactga
    16501 ttaccgcagg catcatgatg tggggtctgg gcgagcctgt aggggcgttg accaacagcc
    16561 tgactcagtg gcttcagggg atgcagcagg gcagcattgt tatgctggcg gtgatcatgg
    16621 gtctgatgct ggcgttcgat atgggcggtc cggttaacaa agtggcctat gccttcatgc
    16681 tgatttgcgt tgctcagggt gtttataccg tggtggccat tgctgccgtg ggtatttgta
    16741 tcccgccgct ggggatgggg ctggcgacgc tgattggtcg taaaaatttc tccgcagaag
    16801 agcgcgaaac cggtaaagcg gcgctggtga tggggtgcgt tggggttact gaaggggcga
    16861 ttcctttcgc cgcagccgat ccgttacgtg taattccttc catcatggtt ggttcagttt
    16921 gtggtgcagt aactgcggcg ctggtcggtg cgcagtgcta tgcaggctgg ggtggtctga
    16981 ttgtgctgcc ggtggttgaa ggtaagttgg gttatatcgc agcagtggct gtcggggcgg
    17041 ttgtgacggc agtctgcgtg aacgtgctga aaagtctggc gcgtaaaaat ggatcttcta
    17101 ctgatgaaaa agaagacgac ctggatttgg attttgaaat taattaattg aggaacgagc
    17161 catgacgaaa attattgcag taaccgcgtg tccatctggt gttgcccata cttatatggc
    17221 tgcagaggcg ctggaaagtg ccgcgaaagc gaaaggctgg gaagtgaaag tagaaaccca
    17281 gggctcgatt ggtctggaaa acgaactgac tgcggaagac gtggcgagcg ccgatatggt
    17341 gattctgact aaagatatcg gcatcaagtt tgaagagcgt tttgctggca agaccatcgt
    17401 gcgcgtcaac atcagcgatg cggtaaaacg tgctgatgcc attatgagca aaattgaagc
    17461 tcacctggcg caaaccgctt aagttcttct cccgctcgca agggcgggtt cgctttccca
    17521 caggagttcc tcatgacgaa tcgtatctct cgcctcaaaa ctgcactgtt tgccaatacc
    17581 cgtgaaatct cgctggagcg tgcgctgctt tataccgcca gccatcggca aaccgaaggc
    17641 gaaccggtga tattgcgccg ggcgaaagca acagcgtata tccttgaaca tgttgaaatt
    17701 tcgattcgtg atgaagaact gattgccggt aaccgcaccg taaaaccgcg cgccgggatt
    17761 atgtcgccgg aaatggaccc ttactggctg ctgaaagagc tgggtcaatt cccgacgcgt
    17821 ccgcaggacc gttttgctat cagcgaagaa gataaacgta tctaccgcga agagttgttc
    17881 ccgtactggg aaaaacgttc gatgaaagat ttcatcaacg ggcagatgac ggatgaagta
    17941 aaagccgcga ctagcacgca gattttcagc atcaaccaga cggataaagg ccaggggcac
    18001 attattattg attacccgcg cctgctgaat cacgggctgg gggagctggt ggcacagatg
    18061 cagcaacatt gtcagcaaca gccggagaat cacttttatc aggcagcgct gttactgctc
    18121 gaagcctcgc agaaacacat tttgcgttac gccgaactgg cggaaacgat ggcggcaagc
    18181 tgcactgatg cccagcgtcg cgaagaactg ttgactattg cggagatctc ccgccataac
    18241 gccgaacata aaccgcagac gttctggcag gcgtgccagt tattctggta catgaacatc
    18301 attctgcaat acgaatccaa cgccagttcg ctgtctttag ggcgcttcga ccagtatatg
    18361 ttgccgttct atcaggcatc tttaacccag ggcgaagatc cggcgttcct gaaagaactg
    18421 ctcgaatctt tatgggtgaa atgcaacgac attgtgctgt tgcgctccac cagcagcgcg
    18481 cgttatttcg caggtttccc gactggctat accgcactgc tcggcgggtt aaccgagaac
    18541 ggacgtagcg cggtgaacgt gctttcgttc ctctgcctcg atgcctatca aagcgtacaa
    18601 ttaccgcaac cgaacctagg ggtgcgcact aacgccttga tcgacacgcc gttcctgatg
    18661 aaaaccgcag aaaccattcg cctcggcacc ggtattccgc aaatctttaa cgatgaagtg
    18721 gtggtgccag cgttcctcaa ccgtggcgtt tcgctggaag atgcgcgcga ctattccgta
    18781 gtgggctgtg tggaattatc tattcccggc agaacctatg gcttgcatga catcgcgatg
    18841 tttaatctgc tgaaagtgat ggaaatctgc ctgcatgaaa atgaaggcaa tgctgcgctg
    18901 acttatgaag gtttactgga gcagatccgc gccaagatca gccactacat caccctgatg
    18961 gttgagggca gcaatatttg tgatatcggc catcgcgact gggcacctgt accgctgctc
    19021 tcgtctttta tcagcgattg tctggaaaaa ggtcgcgata ttaccgatgg cggcgcgcgt
    19081 tataacttct ccggcgtaca gggaattggt atcgccaacc tgagcgattc tctccatgcg
    19141 ttgaaaggga tggtttttga tcaacagcgt ttaagttttg acgaattgct gtcggtatta
    19201 aaagccaact ttgcaacgcc agaaggcgaa aaagtccgcg ctcgcttaat taaccgcttt
    19261 gagaaatacg gtaacgatat cgacgaggtg ggtaacatta gcgccgaact gttgcgccac
    19321 tactgcaaag aagtggaaaa ataccagaac ccgcgcggcg gttacttcac gccgggatcg
    19381 tataccgttt ctgctcacgt cccgttggga tcggtggttg gcgcgacgcc agacggtcgt
    19441 tttgccggag aacagttggc agacggcggc ttgtcaccca tgctgggcca ggacgcacaa
    19501 gggccaacag cggtactgaa gtcagtcagt aagctcgata acacgctgct gtctaacggt
    19561 acgttgctga acgtgaaatt cactccggcg accctggaag gtgaagcggg attacgcaaa
    19621 ctggccgact tcttacgggc gtttacccag cttaagttgc agcatattca gtttaacgtg
    19681 gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac aagattatgc cgggctggtg
    19741 gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt cgaaggagat ccaggatgac
    19801 atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa acgcgccgca atgatgtggc
    19861 gcgcattttc aacattcagc gttattcact gaatgacggc gagggcattc gtacggtggt
    19921 cttttttaaa ggctgtccgc atctttgccc gtggtgtgct aatccggagt cgatctccgg
    19981 caaaatccag acggtacgca gagaggcgaa atgtctgcac tgtgcgaaat gtttgcgtga
    20041 tgcggatgaa tgcccctccg gggcgtttga acggattggt cgcgatatca gccttgacgc
    20101 gctggaacgg gaagtgatga aagatgacat tttctttcgc acgtccggcg gcggcgtcac
    20161 gctttctggc ggcgaagtgt taatgcaggc ggagtttgct acccgttttt tacagcggct
    20221 gcggttgtgg ggcgtgtcat gcgccattga aactgccgga gacgcgccag ccagcaagct
    20281 gttaccgctg gcgaaattgt gcgatgaagt gttgttcgat ttaaaaatta tggacgcgac
    20341 tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg ctggagaatc tgcgtttgct
    20401 ggtgagtgag ggcgtcaacg tgatcccgcg tttaccgctg atccctggtt tcacgctcag
    20461 ccgggagaat atgcagcagg cgctggatgt actgatcccg ctgaaaatca ggcagatcca
    20521 tctgttaccg tttcatcagt acggcgaacc gaaataccgc ctgctgggga aaacatggtc
    20581 gatgaaagag gtgcctgcgc cgtcgtcagc cgatgtgaca acgatgcgcg aaatggcaga
    20641 acgggccgga tttcaggtta ccgtgggagg ttaaaatggc atacctggtg gcagtaaccg
    20701 cctgcgtaag cggcgtggcg catacatata tggcggcgga acggctggaa aagttgtgcc
    20761 tgttagagaa gtggggagtc agcattgaaa ctcagggcgc gctgggaacg gagaatcgtt
    20821 tagcggacga ggatatccgt cgggcggatg ttgctctgtt gattacggat atcgagcttg
    20881 ccggtgccga gcgatttgaa cattgccgct atgtgcaatg cagcatctac gcattcctgc
    20941 gtgagccgca gcaggtaatg agcgcggtgc gcaaagtgct ttctgcgccg cagcaaaccc
    21001 atcttattct ggagtagtcg gtttttctgt cagttggctg tggtactgcc ggcgatattc
    21061 cgacggcgag cgttcggtgt ttttacgaaa cagacggcag aagtagttgc tgtcgacaaa
    21121 gccgcaggcg tgcgccactt cttttacctt caggtcatag cctttcaata acgtcttagc
    21181 gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca atggcccccg ttttttgaaa
    21241 caggtgcgag agatagtttg gtgaaatata gaatgcctgt gcgacagatt cgcgggtaag
    21301 cgcggaggca tagcgttcgt cgatataatc gcgaatagct tcgaatagtg cctggctgcg
    21361 tgaggcggtc tggatttggc tgccaagcaa atcgcggcag tggctaagca ggctggcgac
    21421 gataagcctt gccgtttgct gctcctgcgg ctgcatttgc atttcattga gcgtttgtag
    21481 cagaaaagaa cctatgcgtg ggccgcgtcg ggcgacgtgt tgcttcgcca gattttgata
    21541 ttgtttgcca tcccattgca cgacgctgaa gccgagttgc tgtttgccaa acagcacgct
    21601 aaaggtagta gcaggggctt gccattgtgg aaaattccag ccaccagcgg caacgtacag
    21661 cacatcgccg ggaactaacg ttgctccagc gccggtatca acaaactcac cttccagcac
    21721 aatttccagc cgtggaaaat cgacctgata cgcaagatcg ggcaccgggc cgttactgct
    21781 ggcaaagtaa atctggcgca gggacagcgg gccgttgatc aggcgggaga gcagatagct
    21841 gacgtcgtga tacatgttat tcccttaggt atggagacgg gaaaacagta actggcatta
    21901 ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt cgatgtaaac attgcggcag
    21961 ttgtattgcc ggatgtggcg taaacgcctt atccagtcta ccgccgcgat ctgtaggccg
    22021 gataagacgc gtcaagcgtc gcatccggca aatagcgcct ggctgataat tactgattac
    22081 ctacctggtc gccatacggc agcgtgtcgt aatcgatcag cgcgtttttc ttgtacgggt
    22141 taccaatcca gcgggtattt tctttgaact gcggattcac cactgaacgg gttggatcgt
    22201 aaccgtcgta agataagccc gctaaatctg accaggtgtg gatcagttcc gcaaggctgt
    22261 atttacgatc aacatcctgc gagaaatcac gcggatgagt cgcttgccat ttttccgacg
    22321 tccacagcag gaacggaatg gtgtacatat gacgcgtcgg gttgtcctca ttacgcccct
    22381 gggttttatg aggcggcgtg tcgtaaacct cttcaccgtg gtcagagaag taaaccagga
    22441 aaccgttcgg gtctgctgct ttaaagtctt taatcaggct ggcaaccaca tgatcgttat
    22501 acaggttagc gttgtcataa tcgttatatg actccagctc ttccgcgttt aatcccggcg
    22561 gaacatgatc ggtattgcca tcaaacttgc cctgattttc cgggtagcgg tatttgtatt
    22621 tgatatgcgt acccagcaga tgaacgatga tcagtttctt cggcgcaggg tccttcagca
    22681 cttcctggaa cggcttcagc acgttagtgt cgtattcacg cgcactctgc gtacgttgct
    22741 ggttcatgta gtactgcttg tcggtctggc gcgaaaatac cgtcagcatg gtattgcggg
    22801 cggtcatcgt ctgctggttg gtgatccaga aggttttata acccgcctgt ttcatcatgt
    22861 tcatcagcga cggctgcgtc agatacagat ccgggttctt ttcattggca aaggtcagcg
    22921 cctgttgcag gatttcaatg gtgtacggac gagacgtaac tacgttatta aacacggtca
    22981 gattcggatc ggttttatgc agcgcattca gctccggcgt ggtttcacgc ggataaccgt
    23041 acagactcat gcgtccacgc tgggttgact cgccaatcac cagcactaag gtgcgcggtt
    23101 cgttacccga ttcatctttg aaattagcca gcggcggcaa ggcattattt tcattcagta
    23161 gctttgttag cgagtttagt tgctgacgat actgataata gccggtcagg aattgccacg
    23221 gtgcggcagg ctccatacgc gaggccaggt tatccaacgt tttctcaaac ggcttgtttt
    23281 tgataaacat attcatggcg atcggatgca gaatcaagcc ataaagcagg gcaaaagaga
    23341 caacataacg ccacggcttt ggaatataga ccgggcgcag gcgtgtccac agcagaactg
    23401 ccaccgccgt ataggccagc gcgataagca caattttcag gctgaaatac tggcttaaat
    23461 actcgctggc ttcgttggtg ttggtttcga acatcacaaa cagaacgctc tgcgagaact
    23521 cctgaccgta gatgacgtag tagcacagcg ccgccagaga ggccgcccat agcaccacgc
    23581 cgatcactgc ggcaataatt ttgatccgct tcggaaagag gaataccgga attaaccaca
    23641 gcgagctgaa taacagcgag tcgcgaatgc cgttggtgcc actataacca ctgatgtaaa
    23701 taatggcctg tagcagagta gagaaaaacc aaaagtagag caatgcccaa cccagggctt
    23761 tccagctaaa aagaggttta gcctggactt ctgtggaatg catagtaaga acctgtcttg
    23821 aaaaaaatat cgccgaattt accgacaatt ccttaaggat atctgaaggg atatccagaa
    23881 tttgaataaa atgcagacag aaatatattg aaaacgaggg tgttagaaca gaagtatttc
    23941 agaaaaccct cgcgcaaaag cacgagggtt tgcaggagag gaagattagc cggtattacg
    24001 catacctgcc gcaatcccgg caatagtgac cattaacgcc tgctcgacgc gaggatccgg
    24061 ttcctggcct tctttttctg cctggcggga gcggtgcagc aactcggctt gcaatacgtt
    24121 cagcgggtcg gtgtaaatat tccgtagctg aatagactct gcaatccacg gcagatcggc
    24181 catcagatgg gaatcgttgg caatcgccag caccactttg atgtcttctt cttgcagatt
    24241 gcgtaactct ttacctaacg gccacagtgt tttgtctacc aggcgttggt catagtattc
    24301 cgccagccac aggtctgctt tggcgaagac catctccagc atgccgagac gcgtcgagaa
    24361 gaatggccaa tcgcggcaca tagcctccag ctcgctctgt ttgccatctt cgaccacttt
    24421 ttgcagcgcc gtacctgcac ccagccaggc ggggagcatc agacggtttt gcgtccaggc
    24481 gaagatccac ggaatggcgc gtagtgactc gacgccgccg gttgggcgac gtttcgccgg
    24541 acgtgaaccc aacggcagtt tgcccagttc ctgttccggc gtagcggagc ggaagtaagg
    24601 cacaaaatct ttgttttcac gtacgtagcc acggtagaga tcgcaggaga tgactgatag
    24661 ttcatccata atgcgacgcc agctctcttt cggctcaggt ggtggcagca ggttggcttc
    24721 cagtatcgcc ccggtgtaaa gcgacaggct gctgacggtg atttctggca gaccatattt
    24781 aaagcggatc atctcgccct gttcagtcac gcgcaggcca cctttcaggc ttcctggcgg
    24841 ttgtgacagc agcgccgcat gagcaggtgc gccgccgcga ccaatggaac cgccgcgacc
    24901 gtggaacaac gtcagctcaa tacccgcttt ttcgcaggtt ttgattaatg catcctgtgc
    24961 ctgatattgc gcccaggacg ctgccatcac gcccgcatct tttgctgagt cggaatagcc
    25021 aatcatcacc atctgtttgc cctgaatcag gccgcgatac cagtcgatat tcagcaactg
    25081 agtcatgaca tcgttggcgt tgttcaggtc atcgagagtt tcaaacagcg gagcaaccgg
    25141 catcgcaaac ccgatacccg cttctttcag cagcaggtgg acagccagca cgtcggacgg
    25201 cgttttcgcc atcgagatca cgtaggcggc aatggagcct tgcggtgctt cggcaatcac
    25261 ctggcaggta tcgagcactt cgcgcgtttc ggcgcttggt tgccagttgc gcggcagaag
    25321 cggacgtttg gagttcagtt cgcggatcag gaacgcctgt ttgtcggcct ctgaccagct
    25381 ttcgtagtcg ccgataccga ggtagcgggt cagctcgccc agcgcttcgg tatgacgcgt
    25441 gctctcctga cggatatcaa tacggaccag cggtacgccg aaacatttca cgcggcgcag
    25501 ggtgtcgagc agatcgccgt tggcgataat acccatgcca cacgcctgaa gtgactggta
    25561 gcaagcgtag agcggttccc acagttcttc gttttgtgtc agcaggcctt ctggttttgg
    25621 cagttcttcg cctttcaggc gcgcttccag ccatgcctgt gtcgccatca ggcgagaacg
    25681 caggtttttc atcagatagc gatacggttc tgcggcacct tcttcgccaa ccagcgccag
    25741 cagttcaggg gtcgcttcaa ccatcgacag ttcagaaacc agaacctgaa tatctttcag
    25801 gaacagatcg gtggctttcc agcggctgag cagcaggacg tggcgggtga tatcggcagt
    25861 gacgttcggg ttgccgtcgc ggtcaccgcc catccacgag gtaaaacgta ccggaacaaa
    25921 ttcgacgggc agtttgtagc cgaggttctc ttccagttgt tcgttcagtt cgcgcaggta
    25981 attcggtacg ccttgccaca ggctgttttc tactacggca aagccccatt tggcttcatc
    26041 taccgggctt ggacgcagct tacggatttc atcggtatgc catgactggg cgatcaactg
    26101 gcgcaggcga cgcatcagct ggttgtgttc gtagtcagcg atatctttgt tatcgagctg
    26161 ttttaagcag gcgttcactt ccaccatttt gtggatcagt gtacgacggg taatttcggt
    26221 tgggtgagcc gtgaggacca gttccagcga cagcgattcc actgcttttt tgatggtgtc
    26281 ttcgctcagt tccggctggt ttttcagttt acgcagggtg cgggcgatca cttccgggtt
    26341 gctggcagct tcgcctttcg gcgaaatgct gtggtattgc tcggcggtgt tggccaggtt
    26401 caggaactga ctaaacgcac gcgcaacggg cagcagctcg tcgttcgaca aattttgtaa
    26461 ggtggtgagt aactcctggc ggttagcatc attgccagcg cgtgaagatt tggacaactt
    26521 acggatagtt tctacgcgtt caagaatgtg ttctcccaac gcatccttga tggtttctcc
    26581 cagcactttg ccgagcatac tgacattact acgcaatgcg gaatattgtt cgttcatatt
    26641 accccagaca ccccatctta tcgtttgata gccctgtatc cttcacgtcg cattggcgcg
    26701 aatatgctcg ggctttgctt ttcgtcgtct tttataaagc cacgtaaaag cggtgacgtc
    26761 aaatgctgcg aaatcgcttc agcaaacgaa taaatagcag gaatttacgt cattaaattc
    26821 acgacgcttt aaataagcgt aacttatggg aatgttaaaa aatcgcctca agtaacacca
    26881 aaggtgtagg tcggataaga tgtgcaagca tcgcatccga cattattgcg gcactggagt
    26941 ttggcaacag tgccggatgc ggcgcaagcg ccttattcgg cctacgtttt aatgccagca
    27001 aaaatggtga atgacctggg ttatcagttc gcgggtgggc ttgataaacc gtgtttccag
    27061 atattcatca ggctgatgag cctgattaat tgagccaggc cccagaacca gcgtcgggca
    27121 taacgtttga ataaacggcg cttcggtaca gtaattcacc acttcggttt ttgctccgag
    27181 caatttctca accacttcaa ccagttgatg attcggtggg cattcatagc cagggatcgg
    27241 cggatgcagc tcgtcgaccg tcagacgacc cggccagcgt tcgctcaccg gagccaatgc
    27301 atcgttgagc aaaccattaa gttcattgag tgtcatgcca ggcagcggac gaatatccat
    27361 atgcaactca caccaagcgc aaatacggtt agaagcgtcg ccaccgtgaa tatgcccgag
    27421 gttgagcgta gggtatggca cggtaaacgc ttcgtagtga taacgttctt tcaggttatc
    27481 gcgcaattgc aaaatatgcc cgatggcgtc gtgcattagt tctatagcgt taactccgcg
    27541 tgctggatcg ctggagtgcc ccgactggcc ctgaatacgg atggcgttag agatatgacc
    27601 tttatgtgcg cgtaccggtt gtagtgacgt cggttcgcca atgatggcgc aatccgggcg
    27661 cagggcggta gtttcggcaa aataacgcgc tccggccata ctggtttctt catcagcagt
    27721 cgccagaatg tagagcggtt ttttcagttt cgtgacgtcg acatcgcgta gcgcatcaag
    27781 gataaacgca aaaaagcctt tcatgtcggc ggtgcctaag ccgtaaagct tgccgtcatg
    27841 ctccgtcagt gtaaacggat cgcgcgtcca gcgaccgtca tcaaatggca ccgtatcggt
    27901 atgccccgcc agcaacaagc cgccagcccc ctgtccggta cttgccagca tattaaattt
    27961 gttgcgagtt cctggtacag gctgcacttc cacattgaag cctaaatctt taaaccagtc
    28021 cgccagcaga gtgattaaat ctgcattgct ttgatcgagt gcctcttccg tggcgcttat
    28081 tgaaggtgtg gcaatcagag cacggtaaat ctcgataaat ggcggtaatt tgtttttcat
    28141 tgttgacaca cctctggtca tgatagtatc aatattcatg cagtatttat gaataaaaat
    28201 acactaacgt tgagcgtaat aaaacccacc agccgtaagg tgaatgtttt acgtttaacc
    28261 tggcaaccag acataagaag gtgaatagcc ccgatgttga atacgctgat tgtgggtgcc
    28321 agcggctacg ctggcgcaga gctagtgacc tgtgtaaatc gccatccgca tatgaacata
    28381 accgctttga ctgtttcagc gcaaagcaat gatgcgggaa agttaatctc cgatttgcat
    28441 ccgcagctaa aaggcatcgt tgatctgccg ttgcagccga tgtcggatat cagcgagttt
    28501 agccctgggg tggacgtggt gtttctcgcc accgcccatg aagttagcca cgatttagcg
    28561 ccgcagtttc ttgacgcggg ctgcgtggtg ttcgaccttt ccggcgcgtt tcgtgttaac
    28621 gacgccacct tctatgaaaa atattacggc tttacccatc aatacccgga actgctggaa
    28681 caggcagcct acggtctggc ggagtggtgc ggtaataaat taaaagaagc gaatttgatt
    28741 gcggtgccag gctgttatcc gacggcagca cagttggcgc tgaaaccgtt gattgatgcc
    28801 gatcttcttg acctcaatca gtggccggtg atcaacgcca ccagcggcgt gagcggtgca
    28861 ggacgtaagg cagcgatttc aaatagtttt tgtgaagtta gcctgcaacc gtatggcgtc
    28921 tttacccatc gccatcaacc agagatcgcc acacacctcg gtgctgacgt tatcttcacc
    28981 ccgcatctgg gcaatttccc gcgcggcatt ctcgaaacca ttacctgccg cctgaaaccg
    29041 ggtgtgaccc aggcgcaagt ggcgcaagcg ttacagcagg cgtatgccca taaaccgctg
    29101 gtgcggctgt atgacaaagg cgttccggcg ctgaaaaatg tcgttgggct accattttgc
    29161 gatatcgggt ttgccgttca gggcgaacat ctgattattg tggcgaccga agacaactta
    29221 ctgaaaggcg cggcggcaca agcggtacag tgcgccaata ttcgtttcgg ctatgcggaa
    29281 acgcagtctc ttatttaagg gtgcaatgat gaatccatta attatcaaac tgggtggcgt
    29341 actgctggat agtgaagagg cgctggaacg tctgtttagc gcactggtga attatcgtga
    29401 gtcacatcag cgtccgctgg tgattgtgca cggcggcggt tgcgtggtgg atgagctgat
    29461 gaaagggctg aatctgccgg tgaaaaagaa aaatggcctg cgggtgacgc ctgctgatca
    29521 gattgacatt atcaccggag cactggcggg aacggcaaat aaaaccctgt tggcatgggc
    29581 gaagaaacat cagattgcgg ccgtaggttt gtttctcggt gacggcgaca gcgtcaaagt
    29641 gacccagctt gatgaagagt taggtcatgt tggactggcg cagccaggtt cgcctaagct
    29701 tatcaactcc ttgctggaga acggttatct gccggtggtc agctccattg gcgtaacaga
    29761 cgaagggcaa ctgatgaacg tcaatgccga ccaggcggca acggcgctgg cggcaacgct
    29821 gggcgcggat ctgattttgc tctccgacgt cagtggcatt ctcgacggca aagggcaacg
    29881 cattgccgaa atgaccgccg cgaaagcaga acaactgatt gagcagggca ttattactga
    29941 cggcatgata gtgaaagtga acgcggcgct ggatgctgcg cgcacgttgg gccgtccggt
    30001 agatatcgcc tcctggcgtc atgcggagca gcttccggca ctgtttaacg gtatgccgat
    30061 gggtacgcgg attttagctt aagttttgtt ggccggaggc gcagctttcc ggcattgaat
    30121 ttcaaaataa ggaaacagag ttatggcact ttggggcggg cgttttaccc aggcagcaga
    30181 tcaacggttc aaacaattca acgactcact gcgctttgat taccgtctgg cggagcagga
    30241 tattgttggc tctgtggcct ggtccaaagc cctggtcacg gtcggcgtgt taaccgcaga
    30301 agagcaggcg caactggaag aggcgctgaa cgtgctgctg gaagatgttc gcgccaggcc
    30361 acaacaaatc cttgaaagcg acgccgaaga tatccatagc tgggtggaag gcaaactgat
    30421 cgacaaagtg ggccagttag gcaaaaagct gcataccggg cgtagtcgta atgatcaggt
    30481 agcgactgac ctgaaactgt ggtgcaaaga taccgttagt gagttactga cggctaaccg
    30541 gcagctgcaa tcggcgctgg tggaaaccgc acaaaacaat caggacgcgg tcatgccagg
    30601 ttacactcac ctgcaacgcg cccagccggt gacgttcgcg cactggtgcc tggcctatgt
    30661 tgagatgctg gcgcgtgatg aaagccgttt gcaggatgcg cttaagcgtc tggatgtcag
    30721 cccgttaggt tgtggcgcgc tggcgggaac ggcttatgaa atcgaccgtg aacagttagc
    30781 gggctggctg ggatttgctt cagcaacccg taacagtctc gacagcgttt ctgaccgtga
    30841 ccacgtgctg gaactgcttt ctgctgccgc tatcggcatg gtgcatctgt cgcgttttgc
    30901 tgaagatctg attttcttta acaccggcga agcggggttt gtggagcttt ctgaccgcgt
    30961 gacttccggt tcatcattaa tgccgcagaa gaaaaacccg gatgcgctgg agctgattcg
    31021 cggtaaatgc ggtcgggtgc agggcgcgtt aaccggcatg atgatgacgc tgaaaggttt
    31081 gccgctggct tacaacaaag atatgcagga agacaaagaa ggtctgttcg acgcgctcga
    31141 tacctggttg gactgcctgc atatggcggc gctggtgctg gacggcattc aggtgaaacg
    31201 cccgcgttgc caggaagcgg cgcaacaggg ttacgcgaac gccactgaac tggcggatta
    31261 tctggtggcg aaaggtgtgc cgttccgcga ggcacaccat attgtgggtg aagctgtggt
    31321 ggaagccatt cgtcagggca aaccgctgga ggagctgccg ctcaccgagt tgcagaaatt
    31381 tagtccggtg attggtgaag atgtctatcc gattctgtcg ctgcaatcgt gcctcgacaa
    31441 gcgtgcggca aaaggcggcg tctcaccaca gcaggtggcg caggcgattg cttttgcaag
    31501 ggcgcggttg gggtaagaac atttatatgt acaaatttga gcctggctta tcgccgggct
    31561 tttttatggc aaaaaaaagc ggatcctgga gatccgcaaa agttcacgtt ggctttagtt
    31621 attcgagttg agaaactctc gaaacgggca gtgacttcaa gggttaaaag aggtgccgct
    31681 ccgtttctgt gagcaattat cagtcagaat gcttgatagg gataatcgtt cattgctatt
    31741 ctacctatcg ccatgaacta tcgtggcgat ggaggatgga taatgaatat tcgtgatctt
    31801 gagtacctgg tggcattggc tgaacaccgc cattttcggc gtgcggcaga ttcctgccac
    31861 gttagccagc cgacgcttag cgggcaaatt cgtaagctgg aagatgagct gggcgtgatg
    31921 ttgctggagc ggaccagccg taaagtgttg ttcacccagg cgggaatgct gctggtggat
    31981 caggcgcgta ccgtgctgcg tgaggtgaaa gtccttaaag agatggcaag ccagcagggc
    32041 gagacgatgt ccggaccgct gcatattggt ttgattccca cggttggacc gtacctgcta
    32101 ccgcatatta tcccgatgct gcaccagacc tttccaaagc tggaaatgta tctgcatgaa
    32161 gcacagaccc accagttact ggcgcaactg gacagcggca aactcgattg cgtgatcctc
    32221 gcgctggtga aagagagcga agcattcatt gaagtgccgt tgtttgatga gccaatgttg
    32281 ctggctatct atgaagacca cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg
    32341 gcaggggaaa aactgctgat gctggaagat ggtcactgtt tgcgcgatca ggcaatgggt
    32401 ttttgctttg aagccggggc ggatgaagat acacacttcc gcgcgaccag cctggagacg
    32461 ctgcgcaaca tggtggcggc aggtagcggg atcactttac tgccagcgct ggctgtgccg
    32521 ccggagcgca aacgcgatgg ggttgtttat ctgccgtgca ttaagccgga accacgccgc
    32581 actattggcc tggtttatcg tcctggctca ccgctgcgca gccgctatga gcagctggca
    32641 gaggccatcc gcgcaagaat ggatggccat ttcgataaag tgttaaaaca ggcggtttaa
    32701 accgtttaac gcagctaccc gataggcttc cgccatcgtc gggtagttaa aggtggtgtt
    32761 gacgaagtac tcaatagtgt tgccgccacc tttctgttcc ataatcgcct gaccgatatg
    32821 aataatttcg gcagcgcgct cgccaaagca gtgaatgccc agaatctctt ttgtttcccg
    32881 atggaacaaa attttcagcg tgcccacgtt catgccgacg atttgtgcac gtgccagatg
    32941 tttgaactgg gcgcggccca cttcatatgg cactttcatc gcggtcagct gctgttcggt
    33001 tttgcccaca gagctgattt ccgggatggt gtaaatgccg gtagggatat cttcaatcag
    33061 atgcgcggtg gcttcgcctt ttaccagcgc ctgcgcggca atgcgcccct ggtcataggc
    33121 cgctgatgcc aggctcggat aaccaatcac gtcgcccacc gcgtaaacgt gcggctgtgc
    33181 ggtctgatac atgctgttga ccttcagctg tccgcgactg tcagtttcca gcccaatgtt
    33241 ctgtaacgcc agcgaatcgg tattaccggt acgaccgttg gcatagagca ggcagtcagc
    33301 tttcagtttt ttacccgact tcagatgcat gatcacaccg tcgtcacagc cttcgatctt
    33361 ctcgtactct tcgttgtgac gaatcactac gccactgttc cagaagtgat aggagagaga
    33421 atctgacatc tcttgatcga gaaatgccag caggcgatcg cgggtgttga tcagatccac
    33481 ttttacatcc ataccgcgga agatcgacgc atattcacag ccgatcactc cagcaccata
    33541 gataagtaca tggcgcggtt cgtggtgcat gctgagaatt gagtcgctgt cgtaaatgcg
    33601 tggatgggtg aaatcaacat ctgttggatg atatggacga gagccgcagg caataacaaa
    33661 tttttcagcg gttagtgttt caacgctgcc gtccgggcaa tccagcgcca acgtatgctc
    33721 gtcaacaaag cgagcgttcc cctgcaatat ttcacagtga ttacgttcgt aaaatccctg
    33781 acgcatgcgc gtttgttgat taatcacgtt atcggcatgg ttaaggatat cggcaaaaga
    33841 agagcggagc agtcgggaat ggtcgctgta tagtgggttt tgattgaatt ctataatgcg
    33901 gctgacggcg tgacggagag ctttcgacgg gatggtgccc cagtgggtgc aaccgccgcc
    33961 aacattttga taacgctcga taactgcgac gcgcgcacct tgcttaacca ggcccattgc
    34021 agcgccttcg ccgccggggc cggaacctat tactatggca tcgtaatcgt aggaatgtgg
    34081 catggtaggg cttacctgtt cttatacata aaagcaacag aatggtaaca ttttatcgcg
    34141 ggtaagccaa ttgatccccg tcattaatct ggctatagcg tgagcggcct ttgctttgtc
    34201 tgcctccttc ttaacccatt tattcactat taatcgcttt ttattatggc tgatagtttt
    34261 a
//
LOCUS       DAZFDC010000037        33808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFDC010000037 DAZFDC010000000
VERSION     DAZFDC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.037"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(146..1087)
                     /gene="fabY"
                     /locus_tag="ACHJ48_001905"
                     /gene_synonym="yiiD"
     CDS             complement(146..1087)
                     /gene="fabY"
                     /locus_tag="ACHJ48_001905"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO4707470.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1132..1569)
                     /gene="dtd"
                     /locus_tag="ACHJ48_001906"
     CDS             complement(1132..1569)
                     /gene="dtd"
                     /locus_tag="ACHJ48_001906"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO4707471.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1566..2438)
                     /gene="yihY"
                     /locus_tag="ACHJ48_001907"
     CDS             complement(1566..2438)
                     /gene="yihY"
                     /locus_tag="ACHJ48_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO4707472.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2432..3031)
                     /gene="yihX"
                     /locus_tag="ACHJ48_001908"
     CDS             complement(2432..3031)
                     /gene="yihX"
                     /locus_tag="ACHJ48_001908"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO4707473.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3130..3915)
                     /gene="csqR"
                     /locus_tag="ACHJ48_001909"
     CDS             complement(3130..3915)
                     /gene="csqR"
                     /locus_tag="ACHJ48_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4707474.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3949..4845)
                     /gene="yihV"
                     /locus_tag="ACHJ48_001910"
     CDS             complement(3949..4845)
                     /gene="yihV"
                     /locus_tag="ACHJ48_001910"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO4707475.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYKQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVVLATGGDLAESVRFASGVAASKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5013..5909
                     /gene="yihU"
                     /locus_tag="ACHJ48_001911"
     CDS             5013..5909
                     /gene="yihU"
                     /locus_tag="ACHJ48_001911"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO4707476.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFNMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5933..6811
                     /gene="yihT"
                     /locus_tag="ACHJ48_001912"
     CDS             5933..6811
                     /gene="yihT"
                     /locus_tag="ACHJ48_001912"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO4707477.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6827..8068
                     /gene="yihS"
                     /locus_tag="ACHJ48_001913"
     CDS             6827..8068
                     /gene="yihS"
                     /locus_tag="ACHJ48_001913"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO4707478.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVETFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            8135..9037
                     /gene="yihR"
                     /locus_tag="ACHJ48_001914"
     CDS             8135..9037
                     /gene="yihR"
                     /locus_tag="ACHJ48_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO4707479.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            9098..9973
                     /locus_tag="ACHJ48_001915"
     CDS             9098..9973
                     /locus_tag="ACHJ48_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO4707480.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            10081..12117
                     /gene="yihQ"
                     /locus_tag="ACHJ48_001916"
     CDS             10081..12117
                     /gene="yihQ"
                     /locus_tag="ACHJ48_001916"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO4707481.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFRQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            12163..13548
                     /gene="yihP"
                     /locus_tag="ACHJ48_001917"
     CDS             12163..13548
                     /gene="yihP"
                     /locus_tag="ACHJ48_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4707482.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFSGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            13591..14994
                     /gene="yihO"
                     /locus_tag="ACHJ48_001918"
     CDS             13591..14994
                     /gene="yihO"
                     /locus_tag="ACHJ48_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4707483.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            15061..15753
                     /gene="ompL"
                     /locus_tag="ACHJ48_001919"
     CDS             15061..15753
                     /gene="ompL"
                     /locus_tag="ACHJ48_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO4707484.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSGNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15844..17109)
                     /gene="yihN"
                     /locus_tag="ACHJ48_001920"
     CDS             complement(15844..17109)
                     /gene="yihN"
                     /locus_tag="ACHJ48_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4707485.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGITIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(17211..18191)
                     /gene="yihM"
                     /locus_tag="ACHJ48_001921"
     CDS             complement(17211..18191)
                     /gene="yihM"
                     /locus_tag="ACHJ48_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO4707486.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVREAYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKELLTHLICLGDEEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPEKHLLDERLRKEKEDAINQINHVRNVLQQIKQEASHLLNH"
     gene            complement(18199..18909)
                     /gene="yihL"
                     /locus_tag="ACHJ48_001922"
     CDS             complement(18199..18909)
                     /gene="yihL"
                     /locus_tag="ACHJ48_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4707487.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(19126..20949)
                     /gene="typA"
                     /locus_tag="ACHJ48_001923"
     CDS             complement(19126..20949)
                     /gene="typA"
                     /locus_tag="ACHJ48_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO4707488.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            21322..22731
                     /gene="glnA"
                     /locus_tag="ACHJ48_001924"
     CDS             21322..22731
                     /gene="glnA"
                     /locus_tag="ACHJ48_001924"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO4707489.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22905..23954
                     /gene="glnL"
                     /locus_tag="ACHJ48_001925"
     CDS             22905..23954
                     /gene="glnL"
                     /locus_tag="ACHJ48_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO4707490.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23966..25375
                     /gene="glnG"
                     /locus_tag="ACHJ48_001926"
     CDS             23966..25375
                     /gene="glnG"
                     /locus_tag="ACHJ48_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO4707491.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     NKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25487..25597
                     /gene="yshB"
                     /locus_tag="ACHJ48_001927"
     CDS             25487..25597
                     /gene="yshB"
                     /locus_tag="ACHJ48_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO4707492.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25787..27160)
                     /gene="hemN"
                     /locus_tag="ACHJ48_001928"
     CDS             complement(25787..27160)
                     /gene="hemN"
                     /locus_tag="ACHJ48_001928"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO4707493.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(27349..27855)
                     /gene="yihI"
                     /locus_tag="ACHJ48_001929"
     CDS             complement(27349..27855)
                     /gene="yihI"
                     /locus_tag="ACHJ48_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO4707494.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNNTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(27974..28140)
                     /locus_tag="ACHJ48_001930"
                     /pseudo
     CDS             complement(27974..28140)
                     /locus_tag="ACHJ48_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28437..29069
                     /gene="yihA"
                     /locus_tag="ACHJ48_001931"
     CDS             28437..29069
                     /gene="yihA"
                     /locus_tag="ACHJ48_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO4707495.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(29451..32237)
                     /gene="polA"
                     /locus_tag="ACHJ48_001932"
     CDS             complement(29451..32237)
                     /gene="polA"
                     /locus_tag="ACHJ48_001932"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO4707496.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHRDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            32601..33532
                     /gene="yihG"
                     /locus_tag="ACHJ48_001933"
                     /pseudo
     CDS             32601..33532
                     /gene="yihG"
                     /locus_tag="ACHJ48_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(33573..>33808)
                     /locus_tag="ACHJ48_001934"
     CDS             complement(33573..>33808)
                     /locus_tag="ACHJ48_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFO4707497.1"
                     /translation="ETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNT
                     ASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8183 a         8682 c         8326 g         8617 t
ORIGIN      
        1 tgctcctgaa cagtgatgta gcaagacacg tatggagcca acgaagaata aaaatttata
       61 gagttcttat tcaatataac cgaaatagcc aaggactaat ataaatgacc tgaatgagag
      121 aggcagcacc gcccctctcc atcagctact cttcttcgtt cccgccctct tcatacgggc
      181 caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt
      241 cgccaaagat ttcgacctgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga
      301 gatcgccgct taaggcacca aggtcggcta ccgcatgggg tttaccgcta atcggtttgc
      361 tgtagcggat atgcgcatcc gccagaataa tcgttccgcc gaggtggcgt tcgcgcagca
      421 tcagccagat aagcccccaa ccggtgagcg tcgccagtga gaataaactc ccggcaaaca
      481 gcgtatggtg cggattctga ttgccggttt ctggcatggt agtgataaat ttttgcccgg
      541 tatattgctg aatgcgcacg cccatttttt cactaagcgg gatatgttcg taccacgcct
      601 gttgcagctg cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcgacgggct
      661 taatcatcaa aaaatggcga atcggcgtgg tggttggcgt ggtgatttct ccctgattaa
      721 caaaccccag cttggcgaaa aactccaccg cgtcttcacg ggcgctacag gtcacgcgct
      781 taacgccttc ctgacgcgcc accgactcca gggtcatcgc catcagcgtg cctaaccctt
      841 tgtcctgcac gtcgggatga acggccataa agcgaatgga cgcttcattg tcggcattaa
      901 tatacagtcg gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg
      961 ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca
     1021 tttcccagcg aaactgatag taacgctcta attcttcttc tgtttgtgga acccgaaggt
     1081 gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg
     1141 caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatccg cagcgaagcg
     1201 tcctgtttgc gtgttcatct cttgctgacg gcagcgttcg acgaaatagt catataacgc
     1261 ctctgcgcga tccggtgatg cacctttgga gaaacttggg cgcatccccc gttcggtatc
     1321 tgcggcgagg gtaaactggg aaaccaccag cacactgccg cccgcctgtt gcacgttgag
     1381 attcatcttg ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg
     1441 gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcacc
     1501 aatttcgccc gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa
     1561 tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt gcggtattcc
     1621 ccgagagtga cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa
     1681 acaaagagaa tggggatcac cgccagcaca ccgtaaatga gctgatatga cgggaacatg
     1741 gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca ggagtgcagc aacaaacgcg
     1801 ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta cagcaaccag
     1861 aaggagatcc acgacaacag caacggaaaa atacgcagca cgttgtcgat gacagtattg
     1921 agatcgctcg cccagcgcag ggagagcaaa taggaactga tcgccagact ggcccctgcc
     1981 agcagcggcc ccagcgttaa aatcatccag tacacggcaa acgagtaaat tttgggtcgc
     2041 gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat caacaataac
     2101 gcggtgacga tcagcccgca cgccccaacg gcggtcatct tgttggaatt ggcaacaaat
     2161 tgttcgatat accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag
     2221 tgacgtaact gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca
     2281 acggcaacca gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt
     2341 gtcatattgt cctcatcaat gcgttgccag agtagtttta gccaggccca tagtggacgg
     2401 gtacggtgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg
     2461 ggatggtggt tttatctttc accagaatac tggtaatgcc cagctgatta gctccttcta
     2521 tattatcggc gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca
     2581 aaacatgctg gtaaattcga gcttcaggtt tgcgcatccc cagatcttgc gacagataga
     2641 tatggtcagc agcatcacga atttccgggt attcttccgg ccagaaggtg gtatgcaggc
     2701 ggttagtatt ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg
     2761 cgatcacttc cgggcgcagc gcaacaaaca ccgcctgcca gccgtgagag aactgctcgt
     2821 agcttagcgg tagagccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt
     2881 ccccacgctc atgctgatga aacgcctccc ccatatgaaa actcttctta agcgtagcca
     2941 gcggaatacg cgttaaatcg ctccaggttc ccagcacacg gttaaagtcg atatcgacaa
     3001 tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt
     3061 aactgtagcg ggaaaggatg ggtttggcta tgtggcaagg tatgaagcca ggtcaacagt
     3121 cacaaaaatc tacgcgtctt cctggggaag gatgatttca atttggctgt cttgtaagcg
     3181 tgattttagc gcagcgggcg gcagctcgtc ggtaaagagc gcggtgacct gtgccacgtt
     3241 accaatttca accgccgccg aagcatgata cttagtgtga tcggcaacca gcaggatatt
     3301 tctcgcgtgc gccatcatcg ttttcaccac gttagcttcg tttacatcaa actccatcaa
     3361 cgcgccatcg ctctcaatcg ccccaacgct tgttaccaga taatcagcac gaaaatcggc
     3421 cacaaaggac gccgctgaag ggccaatgat cccgctatta tgagagcgca acgtaccgcc
     3481 gggtaccatc acttcaaagc gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt
     3541 ggtgattatc cgcaaatgat tatggttaag taacgcccgg gcaacatgct caacggtcgt
     3601 accaatggtg ataaatattg ttgaaccatc aggaatatag tctgccaccg cttcggcaat
     3661 cgcttttttt tcctcggttt gcgaaacctc acgctgctcg aacgccgtat taacgacgct
     3721 ggaagcccga cccgcgccac cgtgatggcg cgtaatcagg ccttgctcgc ttaatttacg
     3781 aatatcccgg cgcaccgtct gcgtggagac atccagcaga tttgccagct catcaatatt
     3841 catataccca cgctcggcga tcagcatgag gagttggtcg tgccgcgggt taccggttag
     3901 ttcggtaagg ctcatgaaaa ttcctcgaaa aaccatcacc ctggcatttt atacaaaaag
     3961 tgacaaaaaa gatcgggttt gatcacagtc agggatcccg gctcgtccac cgggacgcgt
     4021 gcatttcgac gccgctacac cgctggcgaa gcggactgac tccgctaaat ccccacctgt
     4081 tgccagcacc accgccaatg cgccgtgaaa aacatcaccc gcacctgtgg tatctaccac
     4141 atcaactttg aaggccggtt gatgttgacg cccgccattt tccagccagt cacagcctgc
     4201 gctgccctgg gtcacataga catgaccatt tgtgagcgtt tgtgcctgtt ttagcgcact
     4261 ggccatctct ttcacgcccg ttaagcgcgc cagacccggt tctgaaaagg ccgcatgatc
     4321 gcttaatgcc accagctcgc tgatatcctg cggcgtaata tcaccgtcca gaacggtcat
     4381 cacgcccgcc tgccgggcca gagtgaaggc ttttttagcg ccgtcgtgcc agcgtacatc
     4441 tgccagcaca acatcccact gagagaaatc aatttcctcc aaccactctg cgtcaggcag
     4501 caggtccggg ctggggtagt taatgattat ccgctcgcct ttggcatcca ccatgatggc
     4561 ggattgcgac gatttcgcct gtttataccg tttggtgtag cgggtgttaa cgccccagga
     4621 ttccagctct gccagcaggc tgttgccggt gtcgtcatca ccgacgcgcc caataaaatc
     4681 aacctgcgct cccagtcttg ccgccgcaac cgctgccgtc gccgctggcc cgccaccaac
     4741 ttccgtataa tttctcgcca cgtatttacc gctctccgtc ggtaaccctt ccacgtaata
     4801 gatgcgatcc atcacggtta tacctacaca agcaacacga atcatggtca ttccttaaac
     4861 attttgggat gcgttcattt taatttcacc aattgtttaa aaagtgacgt ctgtcaattt
     4921 tttgactata aattacaaat acgatcaaaa acagacaatc aaagcgtgtc ataaatgaca
     4981 aaaagtgaca tcactgtatt caggagaagg ttatggcagc aatcgcgttt atcggtttag
     5041 gacaaatggg ttcgccaatg gcgagcaatt tattgcagca agggcaccaa cttcgcgtct
     5101 ttgatgtgaa tgccgaggct gtgcggcatc tggtagacaa aggcgcgact cccgccgcca
     5161 acccggcgca ggccgctaaa gatgccgaat ttatcattac catgttgccg aatggcgatc
     5221 tggtgcgcag cgtgttgttc ggtgaaaacg gcgtttgcga aagcttatct accgatgcgc
     5281 tggtcattga tatgtcgacc atccatccgc tgcaaaccga taaattgatt gccgatatgc
     5341 aagccaaagg cttcaacatg atggatgttc cggtaggccg tacttctgca aatgccatta
     5401 ccggtactct gttactgctg gctggcggca ccgctgaaca agttgaacgt gccacgccga
     5461 tcctgatggc gatgggcagt gagttgatca acgcaggcgg tccgggcatg gggatccgcg
     5521 ttaagctcat caacaactat atgagcatcg cgctcaatgc gctttcggca gaagctgccg
     5581 ttttgtgcga agccctgaat cttcccttcg atgttgccgt caaagtgatg agcggtaccg
     5641 ccgccggtaa aggccacttc accacttcct ggccgaacaa agtcctcagc ggggatcttt
     5701 ctcccgcctt catgatcgat cttgcccata aggatcttgg catcgccctt gatgtcgcca
     5761 accagctgca tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat agccaggcgc
     5821 gcgcagcggg tcgcggtcgc caggactggt ccgccattct ggaacaggtc cgtgtcagtg
     5881 ccgggatgac tgccaaagta aaaatgtaac gactggataa aggaataaat gaatgaataa
     5941 gtacaccatc aacgacatta cgcgcgcatc gggcggtttt gccatgctgg cggtcgatca
     6001 gcgcgaagcc atgcgcatga tgtttgccgc ggctggggca cccgctccgg tagccgatag
     6061 cgttttaact gatttcaaag ttaacgcggc gaagaccctc tcgccttatg cctcggcgat
     6121 tctggtagat caacaattct gttaccgcca ggtggttgag caaaacgcga ttgccaaaag
     6181 ttgcgccatg attgtcgccg ccgatgagtt cattcctggc aacggtattc cggtagatag
     6241 cgtggtcatt gaccgcaaaa tcaatccgct acagatcaaa caagacggtg gcaaagcctt
     6301 aaaactgctg gtgctgtggc gtagtgatga agatgcgcag caacgcctgg atatggtgaa
     6361 agagtttaac gaactgtgcc actcacacgg cctggtaagc attattgagc cagtcgtccg
     6421 cccaccacgt cgtggcgata aattcgatcg cgaacaagcg atcatcgatg ccgccaaaga
     6481 gctgggcgac agtggcgctg acctctacaa agttgaaatg ccgctttacg gtaaaggtcc
     6541 gcaacaagag cttctcagtg cttcacaacg tctgaatgac catatcaata tgccgtgggt
     6601 gatcctctct tccggtgtcg acgaaaaact gttcccgcgt gccgtacgcg tggcaatgac
     6661 agcgggtgca tcgggcttcc tggctggtcg tgctgtctgg gcatcggtcg tcggtttacc
     6721 agacaacgag ctgatgctgc gtgacgtttg cgcaccgaaa ttacaacaac ttggcgatat
     6781 cgtcgacgaa atgatggcta aacgccgtta aaatggggat acaaaaatga aatggtttaa
     6841 cactttaagt cacaaccgtt ggctggaaca ggaaaccgat cgcatctttg attttggtaa
     6901 aaattccgta gtaccgaccg gatttggctg gttaggcaat aaagggcaaa tcaaagaaga
     6961 gatgggcacc catctgtgga tcacagctcg tatgttgcac gtttattccg ttgctgcggc
     7021 gatgggtcga cctggcgctt actcgttggt tgatcacggt attaaagcca tgaacggcgc
     7081 actgcgcgat aaaaaatatg gcggctggta tgcctgtgtg aatgacgagg gcgtggtgga
     7141 tgcctccaaa cagggctatc aacatttctt tgctctgctg ggtgctgcca gcgccgtcac
     7201 aacgggtcac ccggaagcgc gcaagctgct cgattacacc attgaaatta tcgagaaata
     7261 cttctggagc gaagaagagc agatgtgcct ggagtcctgg gacgaagcct tcagcaaaac
     7321 cgaagagtac cgcggcggca atgccaatat gcacgcagtg gaaactttct taattgttta
     7381 tgacgtaacg catgacaaaa aatggctgga tcgcgcgatt cgcgtggctt ccgtgattat
     7441 ccacgacgtc gcaagaaata atcattatcg cgttaacgaa cacttcgata cccagtggaa
     7501 tccgctgccg gattacaaca aagataaccc ggcgcaccgc ttccgcgcgt tcggaggtac
     7561 accaggccac tggatcgaat ggggccgttt aatgctgcac atccacgcgg ccctggaagc
     7621 ccgttgcgaa caaccgccag catggctgct agaagatgcc aaaggtctgt ttaacgccac
     7681 cgtgcgtgat gcctgggcac ccgatggtgc ggacgggatt gtttataccg ttgactggga
     7741 aggaaaaccg gtggtccgcg aacgtgtgcg ttggcctatc gtcgaagcga tgggtacggc
     7801 ctacgcgctc tacaccgtca ccggcgatcg ccagtacgaa acctggtatc agacatggtg
     7861 ggactactgc atcaaatacc tgatggacta cgaaaatggt tcatggtggc aggagctgga
     7921 tgcggacaat aaggtcacca ccaaagtctg ggacggcaaa caggatattt atcacctgct
     7981 gcattgcctg gtgatcccgc gtatcccgtt agcgccaggc ctggctccgg cagttgcggc
     8041 gggtctgctg gatattaacg cgaaataact gactaaaaaa acgacatatc aggctgcacg
     8101 ccgggtatgt cgttattcaa taaaggtacc tcgtatgcaa atcaccaaca tgcattgcag
     8161 tggacagacc gtttcacttg ccgcggggga ttatcacgcg accattgtga ccgtcggtgc
     8221 aggtctggct gaattaacct ttcagggctg tcatttagtt attccacata aacccgaaga
     8281 gatgccgctg gcacatctgg gcaaagtcct tattccgtgg ccgaaccgga tcgccaacgg
     8341 ctgttatcgc tatcaggggc aggaatatca gttacccatt aatgaacatg gttcaaaagc
     8401 cgctattcat gggctgctcg cctggcgtga ctggcaaatc agcgaattaa ccgccaccag
     8461 cgttaccctg acagcttttc tgccgcccag ttacggctat ccgtttatgc tggcttcgca
     8521 ggttgtttat tcgctcaatg cacgcacggg tctttccgtt gagatcgcaa gtcagaatat
     8581 tggtactgtg accgcaccct atggtgtggg tattcatcct tatttaacct gcaacttaac
     8641 atctgttgat gaatatctgt tccagcttcc ggcaaaccag gtttatgccg tcgatgagca
     8701 cgctaatccg acaacgcttc accacgttga tgagctggat ttaaatttca cgcaggcgaa
     8761 aaaaatagcc gcgacaaaaa tcgaccacac ctttaaaacc gctaatgatc tgtgggaaat
     8821 aaccattacc catccacaac aggcgctttc ggttagcctt tgttccgatc aaccctgggt
     8881 acaaatttat agcggtgaaa aattacagcg tcagggactg gcagttgaac ctatgagttg
     8941 cccgcctaac gcctttaatt caggtctcga cttattatta ctggaaccag ggaaaacgca
     9001 ccgcctgttt tttaatattt acgggcaaca caattaattt tattaagaaa agaacgagat
     9061 cattgtttag agttgtattt acacaacagg aaatattatg gactttttta cgttattttc
     9121 accggatggg gtcatgctgg aaacaaacgt ggtatgtatc gttatcgcgt taatgtttct
     9181 ttataccttt gtggggatat gcgcgggatt tggcggtggt ttaaccacta tgccgctcgt
     9241 cacgttaatg ctccccgtaa aaatggcaac accactctcg gtaattgtcg gcaccgcgac
     9301 cgccttgtac gccacctggc tgtcacgtaa agaaacggat tggcgatcgg caatggtact
     9361 gattattttc tcgtttctgg gaatacccgt cggtttatat gcactgtcgt atttacccga
     9421 ccattttatg aaagtgggct taggcggatt ccttatcctg tactcctttt acagtatgtt
     9481 tattccacgc ctgccgattt atgaccgtag ctggatcgcc gcacctatcg gtgcagtagc
     9541 aggcgcatta ggcgcagctt tctcaaccaa cggaccaccg atggttatct acggtatgct
     9601 gcgtaactta ggcccggcgg catttcgtgg cactctgaac gctttcttca ccgcaaacaa
     9661 catcgcggtg atcggcgggc tggcgaccag cggaattttg actgtttcga tctttaaact
     9721 ggtgctgttt tgtattccca ccatgatcct cggttcactg gttggacaat atgtgcataa
     9781 acgtatttca gtcaaagtgt tccgtgtgat ggtcttttta ttgctgattg cctccggttc
     9841 catgttgata aaaggcgcgc tgggaattcc cgccgctgcc gcacttctac ctccgtttac
     9901 cttgttagtt atactgcaac tggtatttgg taaaaaagtg gcggcaatcc gtcaggctga
     9961 agcaagtaaa taatcactta tgcgggttta aatttatatc cgcacacatt ctttaattaa
    10021 ttaaacctgt aataacagga tggcatgaaa tattagcttt atactctatg gagattattt
    10081 atggatacgc cacgcccaca attaccagat tttgaatttc atcagaataa cgacagtttt
    10141 accctacatt ttcgacaacg tcttatttta acccatagca aagataatcc ttgtttatgg
    10201 attggctcag gtatagcgga catcgatatg ttccgcggca atttcagcat taaagataaa
    10261 ctacaggaga aaattgcgct taccgacgcc atcgtcagcc agtcaccgga tggttggtta
    10321 atccatttca gtcgtggttc tgacattagc gccacgctga atatctctgc cgacgatcag
    10381 ggccgtttat tgctggaact acaaaacgac aaccttaacc acaaccgcat ctggctgcgc
    10441 cttgccgctc aaccagagga ccatatctac ggctgcggcg aacagttttc ctacttcgat
    10501 ctgcgcggca aaccgttccc gctatggacc agtgaacaag gcgttggtcg caacaaacaa
    10561 acctatgtca cctggcaggc cgactgcaaa gaaaatgcgg gcggcgacta ttactggact
    10621 ttcttcccac agcctacgtt tgtcagcacg cagaagtatt attgccatgt tgataacagt
    10681 tgctatatga acttcgactt tagtgccccg gaataccatg aactggcgct gtgggaagac
    10741 aaagcaacgc tgcgttttga atgtgctgac acatacatct ccctgctgga aaaattaacc
    10801 gccctgctgg gacgccagcc agaactgccc gactggattt atgacggagt aacgctcggc
    10861 attcagggcg ggacggaagt gtgccagaag aaactggaca ccatgcgtaa cgcgggcgtg
    10921 aaggtcaacg gcatctgggc gcaggactgg tccggtattc gtatgacctc ttttggcaaa
    10981 cgcgtgatgt ggaactggaa gtggaacagc gaaaactacc cgcaactgga ttcgcgtatc
    11041 aagcagtgga acaaagaggg cgtgcagttc cttgcctata tcaacccgta tgttgccagt
    11101 gataaagatc tctgcgaaga ggcggcaaag cgcggttatc tggcaaaaga tgtcgccggt
    11161 ggcgactatc tggtggagtt tggcgagttc tacggcggcg ttgtcgatct caccaatccg
    11221 gaagcctacg cctggttcaa ggaagttatc aaaaagaaca tgattgaact cggctgcggc
    11281 ggctggatgg ctgacttcgg cgagtatctg cccaccgaca cgtacctgca taacggcatc
    11341 agcgcggaaa ttatgcataa cgcctggccc gcgctgtggg cgaaatgtaa ctacgaagcc
    11401 cttgaagaaa cgggcaagct cggcgagatc ctcttcttta tgcgcgccgg ttctaccggt
    11461 agccagaaat actccaccat gatgtgggcg ggtgaccaga acgtggactg gagtctggac
    11521 gatggcctgg cgtcggttgt cccggcggcg ctatctctgg caatgaccgg acatggcctg
    11581 catcacagcg acattggcgg ttataccacc ctgtttgaga tgaagcgcag caaagagctg
    11641 ctgctgcgct ggtgcgattt cagcgccttc acgccgatga tgcgcaccca cgaaggtaac
    11701 cgtcctggcg acaactggca gtttgacggc gacgcggaaa ccatcgccca tttcgcccgt
    11761 atgaccaccg tcttcaccac cctgaaacct tacctgaaag aggccgtcgc gctgaatgcg
    11821 aagtccggcc tgccggttat gcgcccgctg ttcctgcatt acgaagacga cgcgcagacc
    11881 tattccctga aatatcagta cctgttaggt cgcgacattc tggttgctcc ggtacacgaa
    11941 gaaggtcgca gcgactggac gctctatctg ccagaggata actgggtcca cgcctggacc
    12001 ggagaaacct tccacggtgg agaaataact gttgaagcgc ccatcggcaa gccgccggtc
    12061 ttttatcgcg ccgacagcga atgggctgca ctgttcgcct cgttaaaaaa catctaatca
    12121 tgcccgcccg tggggaaact cacgggcaaa tggagaataa cgatgagtca catcacaacg
    12181 gaagatccgg caactttgcg cctgcccttt aaagagaaac tctcttacgg tatcggcgat
    12241 ctggcctcta acatcctgct ggatattggt acgctttatc ttttgaagtt ttataccgac
    12301 gttctggggc tacctggcac ctatggcggc attatctttt tgatctcgaa attctttacc
    12361 gcctttaccg atatgggaac cggcatcatg ttggattccc ggcgtaagat tggcccgaaa
    12421 ggtaagttcc gccctttcat tttgtacgcg tcattcccgg tcaccttatt ggcaatcgct
    12481 aactttatcg gcacaccgtt cgatgtcact ggtaaaacgg tgatggccac tattctgttt
    12541 atgctctacg ggctgttttt cagcatgatg aactgctcct atggcgcgat ggtgcctgct
    12601 attaccaaaa accccaacga gcgcgcatca ctggcggcat ggcgtcaggg aggcgctacg
    12661 ctgggcctgc tgctgtgcac ggtgggattc gtgccggtta tgaatcttat cgaaggtaat
    12721 cagcaacttg gctatatctt cgccgccacg ctgttttcac tgttcggcct gctgtttatg
    12781 tggatctgct actcgggcgt gaaagagcgt tatgtcgaaa cccaaccagc caatccggcg
    12841 caaaagcctg gcctgctgca atctttccgc gcaattgccg gtaaccgccc actgttcatt
    12901 ctgtgcattg ccaacctctg cactttaggg gcgtttaacg tcaagctcgc catccaggtc
    12961 tattacaccc agtacgtact taacgatccc atcctgttgt catatatggg atttttcagc
    13021 atgggctgta ttttcatcgg tgtgttcctg atgcctggcg cagtcaggcg ttttggtaag
    13081 aagaaggtct atatcggcgg cctactgatt tgggtgctgg gcgatctgct caactatttc
    13141 ttcagcggcg gttcggtcag cttcgtggcg ttctcctgcc tggcattctt cggctcagcg
    13201 tttgttaaca gcctgaactg ggcgctggtt tccgacaccg tcgagtacgg cgagtggcgt
    13261 accggcgttc gttcggaagg aacggtctac accggcttca ccttctttcg caaagtgtct
    13321 caggcgctgg ctggtttctt ccccggctgg atgctgacgc aaatcggtta tgtgccgaac
    13381 gtggcgcagg ctgaccacac cattgaaggg ttgcgccagc tgatcttcat ctacccaagc
    13441 gcactggcgg tagtcaccat tgtggcgatg ggctgcttct acagcctgaa cgagaagatg
    13501 tatgtccgca ttgttgaaga aatagaagcc cgtaaacgca cggcgtaata ataataaaca
    13561 atgccctgcg gggcgttata aggagtgatt atgtctgacc ataatccact gacattaaaa
    13621 ctgaatctgc gggaaaaaat cgcctatggt atgggcgacg ttggttcgaa tttaatgctt
    13681 agcatcggta ctctgtatct cctcaaattt tataccgatg aattagggat gccagcttac
    13741 tatggcggga ttatctttct ggtggcgaag ttttttaccg cgtttaccga tatgctcacc
    13801 ggatttttac tcgattcgcg gaaaaatatt gggcctaaag gcaaatttcg tccctttatt
    13861 ttatacgcag cggttccggc ggcgttaatt gcaacgctcc agtttatcgc caccactttt
    13921 agtttgccgg ttaaaacgac gattgccacc gcgctgttta tgatgtttgg actttcatac
    13981 agtctgatga actgctcgta tggtgcgatg atcccggcca ttactaaaaa cccgaatgag
    14041 cgcgcgcagc tcgcggctta ccgccagggt ggtgcaacca tagggctatt gatttgtacc
    14101 gtggcgttta ttccgttgca gtcgcttttt tctgactcaa ccgtcggtta tgcctgtgcg
    14161 gcactgatgt tctccattgg tggctttatt tttatgatgc tgtgctacag aggcgtcaaa
    14221 gagcattatg tggacacagc accaaccgga cataaagcca gtattctcaa atctttttgc
    14281 gcgatatttc ggaatccgcc attgctggtt ttatgcattg ctaacttgtg taccctggcg
    14341 gcatttaata tcaaactggc gattcaggtc tattacaccc agtatgtgct gaatgatatt
    14401 aatttattgt cgtggatggg attcttcagt atgggatgca tcctcgtcgg cgtattactg
    14461 gtgccattaa ctgtaaaatg ttttggtaaa aaacaggttt atttagctgg catggtgcta
    14521 tgggcggttg gtgatatcct gaattatttc tggggaagta actctttcac tttcgtcatg
    14581 ttctcttgta tcgccttttt tggcacggcc tttgtaaaca gcctgaactg ggcactggtg
    14641 ccagataccg tagattacgg tgaatggaaa accggtattc gtgccgaagg ttctgtttat
    14701 accggttata ccttctttcg taaaatttct gccgcacttg ctggcttctt gccaggcatt
    14761 atgctgacgc aaatcggtta tgttcccaac atcgcgcaaa gtgatgcgac attacagggt
    14821 ttgcgtcagc tcatttttat ctggccttgt gcactggcaa ttattgctgc attgacgatg
    14881 ggattctttt acacactcaa tgaaaaacgg tttgcgttga ttattgagga aatcaaccaa
    14941 cgcaaaaata aagagatggc gacagaagaa aaaacggctt ccgtaacgtt ataaatgtca
    15001 atagccgcta tttccatcct ggtgggtggc ggcctcccta cgtttaaaaa tggacttatt
    15061 atgaaaaata ttaatgcaat aattttatta tcttctctga cctctgcctc agtattcgct
    15121 ggcgcatacg tcgaaaacag ggaggcatat aatctcgcct ccgatcaggg cgaggttatg
    15181 ctacgtgtgg gttataactt cgatatgggc gcgggtatta tgttaaccaa tacctacacc
    15241 tttcagcgag aagatgaact aaaacatgga tataacgaaa tcgaaggttg gtatccgtta
    15301 tttaaaccaa ccgataaatt aaccatccag cccggtggct taattaatga taagagtatc
    15361 ggttcgggtg gtgctgttta tctggatgtt aactacaaat ttactccgtg gtttaacctg
    15421 acagtgcgca accgttttaa ccataataac tacagctcaa ccgatttaaa cggtgatctg
    15481 gataataatg acagctacga gattggcaac tactggaact ttattattac cgataagttc
    15541 tcatatacct ttgagccgca ttatttttat aacattaatg atttcaatag cggcaatggc
    15601 acgaagcatc actgggaaat cactaacacc ttccgttacc gcattaacga gcactggctg
    15661 ccctatattg aattgcgttg gttagatcgc aacgtggaac cgtaccaccg cgagcaaaac
    15721 cagattcgta tcgggacgaa gtatttcttc tgatgtgaaa ctgccggatg cggcgtaaac
    15781 gccttatccg gcctacggtt gatacggttt tcgttggccg gattaagtac atccggccat
    15841 taattaagct tcagccgcca gcatctcttt cttacgttgg ctaatacgct taaccagcat
    15901 tacggaaacc accgcaccac agaatgcgaa gcaggccata atgccaaaca cgattttgta
    15961 gccaataatc cccggattta aatccagaat gtagccatac agactgaagc agaacatcgc
    16021 cggggcgtaa ccaataaagc tacccaacgc catcgccgcg cctgttttat tttcagcaat
    16081 ttttgcttcg ccgataggtg caaaaaatac ggctcgctgt gtaaagacta tcgcgccaaa
    16141 gcccagcgta catgccatcc ctaaatagac cggcatactt tcgtgcggca gcataatcaa
    16201 cagtacgagc gcagcggtac tgatgataaa ggtgtagcat agatatttac tcggcgattt
    16261 caggatttta tctgaaatca tgccaccaat cggtccacca atcattttca gacagtattg
    16321 gttaatgatg ccgtaagccc ccaccagcgc aacgggcaat agatagatgt ttttcaggaa
    16381 tggaatgaag aatgtcaggc cacagtaaac cgcgtaaacg aagaagacgt tgaaagcgat
    16441 aagccagata gttttgtctt tcagcaccga ggtcatactg gtgttttgcg atgccccgtc
    16501 ttcttttttc acctcaacgg acggtgcctc ttctttgtca ttcaggacaa agaaaatagt
    16561 aatgcctacg gcaatcacaa taagggaata gaaccagatg cctgctttaa aacctaataa
    16621 gccactgcca aaccaggtaa atactgccaa cgcagaaaat gccaccacgg tatcgacaat
    16681 gccacgccct gtttcgaaga agccaaacaa ccgaccttgt tgttcgctat tgcccaatcg
    16741 acttaccgac ttgagcaaga ccggccagtt catcatgtcg caagtaacgc caaatagcgc
    16801 ccagacaaag aggatgcccc aataccccgg cattgtcgtc aaataaacac ccagtaatcc
    16861 tgtcgcaatg agtgagaatg acatggtgta tctgcgcggt agtttatcgg caaaataaat
    16921 agacagaaaa aagcccactg tggtaacaaa tgagtttacc gacatagcat taccaatttg
    16981 accattggtc aaatggaaat attcctgcat cgggatataa aacgcatctt tcagcgacgg
    17041 taatttataa attgtaccgc cacacagtgt taatagacta aataacgccc atttcttttt
    17101 cgtgagcata acatccccta agcgatttta aaaatcactg ttttaaaggc aataagatac
    17161 cgaccattct tatggaatgg ttttattaac tgcgtatgag ttaaaacttg ttagtggttc
    17221 agaagatggc tcgcctcttg tttgatttgt tgtagtacgt tacgcacatg atttatctga
    17281 ttaattgcat cttctttttc tttacgtaac cgctcatcca gcaaatgctt ttctggcaat
    17341 ggtgtttcac tcatctggcg ataagggatc cacggattat catcttcgtc ttcaaagcgg
    17401 aacgctggag cataataatc aacctcttct tccaggccat atgccgtcac ctgcggttct
    17461 tcatcgccga ggcagataag gtgtgttaat aactctttga acggcatatc tccctgacct
    17521 gaaatacagg ctttatgacc caggccacct tgttctttaa cgatcaaggc atctttgata
    17581 tggacctggg taatatgcgg tgccatcgtt tttaaggcgt ctatgggatg ctcatttgca
    17641 ttgatcatat tcgcaaaatc aaacagtaag gaaagagact ccatctcact ttctttgacc
    17701 agcgacacca actcatgact ttttaaatct tcatgctgtt cgatcgtaaa agtcaggccg
    17761 ctatcctgat acgcttcccg cacataggca atgtcgttag cgatgatcga taatacgtcg
    17821 cgcaaattac cttcataacg tggataaaaa cgtacggacg atgccccagt tttcaacgcg
    17881 atggcgacgg cttcgtcaat agatgcctta tctgaggcgc tggtttcaat atgaatatca
    17941 aggttcagac ggcgggcttt atccccaaag gcagagagtt ctttatcgtc catattacca
    18001 agagaaaaac gctcgccatc cagaacatgg attttgacgc cacgtagatt attttcactt
    18061 gcgatatcaa gtaaatcagc aggcaacact ctttccagcc gcatatttaa atgaaaggca
    18121 taagcatgta aataaagagg aagagtgtcg acacgttgta gaatctttct tgcgttattt
    18181 attgtcacca taaaacacct acagattaat gatgaggtct acggcctcaa atcgccaata
    18241 ttcaatatct atctggacaa tattgccttg ctggtcggcg cgatgctttt caattaagat
    18301 cgctggcata cccgcagaac cgccaagata cttgctgata tcgcccggca ttctgacggg
    18361 tttgaaaatc aattttttta ctaccgtctc tttttggtag cacttttccc agactgcaga
    18421 aaatgagtgg ttttcaagtt gttcaataaa acccggagca acttcaggat taatatatgt
    18481 ttcgtgatag aaaactttat gtccttccag cgccccccag ccggtgatgc gatagagttc
    18541 agttgatggt gtcactgcaa tcagtggcgc gagcgtctcg ggaatatcgc tggtacggtt
    18601 tttctcggta aacccccagg aaggctctcg cccttgctca attgcggccc gctgaaagct
    18661 cgccgacagc tccggactgt aattaaatcg cggctgggtc acgaaccacc ccttacgatc
    18721 cttacggaag attttggatt ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg
    18781 tttaatgccc agcagttctc ccagctctct ttctgaaggg agttttccac ccggcgtggt
    18841 gatgccatct ttcaaccacc gatccagttt ctcttttgca ttttctacgg tggattgatt
    18901 ctccgccatt gtttaatcct tttggtttaa accagatgaa gcatcttacg cttacgccaa
    18961 aaaagaaagc aattaacggc gtatgacttt gcagataatt gtgacaaagc tctctattga
    19021 cgtaaccaca gacttataag ggagatactc gcattggtgt gcgctcacga caggcaaaaa
    19081 aaaacctgcc atcgctggca ggttttttat gactaaaaaa cgaaattaat cgtctttcgg
    19141 tgcgcggttg gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga
    19201 ggtcggagtc acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt
    19261 catgcggata ggcggaacca gaacaacggc ttcgtcagta ccggaagcac gcatgttggt
    19321 cagtttctta ccggtcaggc agtttacagt caggtcgtta gagcggctat gaataccgat
    19381 aatctgacct tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc gatcctgcaa
    19441 accgaacagc gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg
    19501 acgctgaccc acttcacccg gacgcacgtc gtcgtagtgg ctgaaggtgg agtacagcag
    19561 acccgtaccg gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg
    19621 gatcacgtag tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc
    19681 tttacgttcg cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt
    19741 cacgttttca tacggctctt gtttacgacc gtcgatttca cggaagataa ctttcggacg
    19801 ggataccgcc agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag
    19861 ttcgccacga ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc
    19921 aacgttgtgt accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt
    19981 accttcttta ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc
    20041 atcaacggag agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat
    20101 gttcagttcg cccaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc
    20161 gatacgttcc agaccgaggt ggcccagcac tttaccaact ttcgcgttgc gggttttgcc
    20221 ttcgctatcg atgatagtaa cctgctggtt cggcttcact ttaccgcgct tgatgcggcc
    20281 aatgccgata acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg
    20341 accgtcaagg tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt
    20401 catgtcttcc gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta
    20461 aacgatcggg aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac
    20521 ctgatccaca acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat
    20581 cggcttcagg ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc
    20641 gtcaaatgcg tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc
    20701 accaccgaag tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt
    20761 gatagcggtg tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc
    20821 catcacgcgc tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt
    20881 gtctaccagg gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt
    20941 ttcgatcaca actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg
    21001 cactacaaaa caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga
    21061 tcgctttcac ggagcataaa aagggttatc caaaggtcat tgcaccaata tggtgcttaa
    21121 tgtttccatt gaagcactat attggtgcaa cattcacatc atggtgcagc ccttttgcac
    21181 gatgttaagc atgataacgc cttttagggg caatttaaaa gttggcacag ttttcgcttt
    21241 atctttttta cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgacaatga
    21301 ccaatccagg agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac
    21361 gaagtgaagt ttgttgattt gcgcttcacc gataccaaag gtaaagaaca gcacgtcact
    21421 atccctgctc atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc
    21481 tcgattggcg gctggaaagg cattaacgaa tccgacatgg tgctgatgcc agacgcatcc
    21541 accgcagtga ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt
    21601 gaacctggca ccctgcaagg ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa
    21661 gattacctgc gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc
    21721 ttcctgttcg atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac
    21781 gatatcgaag gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt
    21841 ccggcagtga aaggcggtta cttcccggtt ccgccggtag actctgctca ggatattcgt
    21901 tctgaaatgt gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa
    21961 gtagcgactg ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct
    22021 gacgaaattc agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc
    22081 gcgaccttta tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg
    22141 tcgctgtcta aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag
    22201 caggcgctgt actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca
    22261 aacccgacca ccaactctta taagcgtctg gtcccgggct acgaagctcc ggtaatgctg
    22321 gcttactctg cacgcaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa
    22381 gcacgtcgta tcgaagtacg tttcccggat ccggcggcta acccgtacct gtgctttgct
    22441 gccctgctga tggccggtct tgatggcatc aagaacaaga tccatccggg cgaagccatg
    22501 gacaaaaacc tgtatgacct gccgccggaa gaagcgaaag agatcccaca ggttgcaggc
    22561 tctctggaag aagcactgaa cgagctggat ctggaccgcg agttcctgaa agccggtggc
    22621 gtgttcacgg atgaagcgat cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc
    22681 gtgcgtatga ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag
    22741 ttgtactacc cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct
    22801 ctgcatgggc ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag
    22861 ctataatgca ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc
    22921 agcccgatgc tgggcagatc ctcaactcgc tgattaacag tattttgtta atcgatgaca
    22981 acctggcgat ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca
    23041 aattgtttgg tacgccgtta ccggaactgt tgagctactt ctcattaaat attgagctga
    23101 tgcaagaaag tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg
    23161 acggtcgctc gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc
    23221 tggagatggc gccgatggat aaccagcgcc gcttaagcca ggaacagcta caacatgctc
    23281 aacaagttgc tgcccgtgat ttagtgcgtg gcctggcaca tgagattaaa aatccgcttg
    23341 gcggtttacg tggcgcggcg cagctactca gcaaagcatt acctgacccg tcactgctgg
    23401 aatataccaa agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt
    23461 tggggccgca gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg
    23521 tggtaacgct ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgatc
    23581 ccagcctgcc ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg
    23641 tgcgcaatgc gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca
    23701 ccgcgtttca actgacctta cacggcgaac gctatcggct ggcggcgcgg attgatgtgg
    23761 aagataacgg gccaggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca
    23821 gcggccgcga aggtggcacc gggcttggct tatccatcgc tcgtaatttg attgatcagc
    23881 attcaggcaa aattgaattt accagttggc cagggcatac cgagttctcg gtttacctgc
    23941 ctatcaggaa ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga
    24001 tagttccatc cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacaac
    24061 atttgagaac ggcgcggagg tactggaggc gctggcgaac aaaacgccgg atgtgctgct
    24121 ttcagatatc cgtatgccgg gaatggacgg gctggcactg ctcaagcaga ttaaacagcg
    24181 ccatccgatg cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag
    24241 cgcctatcaa caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagccgt
    24301 ggcgctggtt gagcgcgcca tcagtcatta ccaggaacag cagcagccgc gtaatgttca
    24361 gcttaacggc ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg
    24421 tattatcggt cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac
    24481 cggtaaagaa ctggtcgctc atgccctgca tcgccacagt ccgcgagcca aagcgccatt
    24541 tatcgctctg aatatggcgg ctatcccgaa ggatttgatc gaatcagaac tgtttggtca
    24601 cgagaaaggc gcgtttaccg gcgcgaatac cattcgtcag gggcgttttg aacaggctga
    24661 tggcggtaca ttatttctcg atgaaattgg cgatatgccg ctggatgtgc agacgcgttt
    24721 gctgcgcgtg ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt
    24781 ggatgtgcgg attatcgctg ccactcacca gaatctcgaa cagcgggtgc aggaaggtaa
    24841 gttccgtgag gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg
    24901 tgagcgtcgg gaagatattc cccgcctggc gcgccatttt ttacaggttg ccgcgcgaga
    24961 actgggcgta gaagcgaagt tgctgcatcc ggaaaccgaa gccgcgttga cgcgtctggc
    25021 gtggccaggc aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg taatggctgc
    25081 cggacaggaa gtgttgattc aggatttgcc tggcgaactg tttgaatcaa cggttgcgga
    25141 gagtacttcg caaatgcaac cggacagttg ggcgacgctt cttgcgcagt gggcagacag
    25201 agcgctacgt tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac
    25261 gttactgacg accgcgttgc gacatacgca ggggcataaa caggaggcgg cgcggctact
    25321 cggctggggc cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac
    25381 ggcttgtgtg taaagattga ttattgagcg caaattgctg gtattttgcg ctttactgtt
    25441 ccgataagtt cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat
    25501 aattaatctg gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ctgttgctgc
    25561 cgtgctgtgt gccgcgatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc
    25621 tctttgttca tgccagatgc tgcgtgaacg ccttatccgg cctacaaaag caatattttc
    25681 aacaggtttt gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg
    25741 atggcgcttt cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgag
    25801 agaactgctg catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc
    25861 ggatcagcaa gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc
    25921 catcttttgc taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca
    25981 aatcccactg tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa
    26041 tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc
    26101 cttgttcatc cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt
    26161 cgccaatcat gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg
    26221 tgtagccctg gaaattacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat
    26281 ccggacgggc aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga
    26341 tggtttcctg caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt
    26401 tgcgctgagc agcaaaaatg gtcggcagat gcgcatagtt aaagacgctc agacggtcag
    26461 ggtttagctc cgccacgcgt ttcagggtaa aggcgaaact ttccggcgtc tgtttcggca
    26521 ggccgtaaat caggtcgatg ttggtggagg taaagccaat ctcacgcgca tggttaagca
    26581 gcgcaaagat aaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga
    26641 agtcctgcac gcccatactc aggcgattaa agccctcggc gcgtaaatga tcgagtacat
    26701 ccagttcgat ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga
    26761 agttttcgcg cagcagcttc atcaggcggc tgatttgcgc tttattaaga tacgtcggcg
    26821 ttccgccgcc ccagtgcagc tggctgacgt gacgcccggc gaacagcggt gcacgatgga
    26881 cgatttcttg ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa
    26941 tcttattgca gccgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag
    27001 ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt
    27061 ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct
    27121 ggatcagggc cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg
    27181 tctctggcgg ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag
    27241 ccacttttgt cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat
    27301 aaccggaagg gttatcagca gaacgggaag gcccacggcg gcaatccgtt agttgccccg
    27361 cagcagacgc atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata
    27421 agagaggccg agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga
    27481 ttgctcttcg gcactcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg
    27541 ctcatccgtt tctagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt
    27601 cggtttgtgc tgtttggtga ctttttcagt cacgcccaat ggaatagggg ttttactgcc
    27661 aatacgtgga tctttcggtg cgttctggcc tttgctgcct gacgtgttgt ttccgcccgc
    27721 tgcgcggctg cccggcgcat gaccacgacg ttttttctga cgcttgcggt cacgagcttc
    27781 ctgattcagc tcttcgcgcg ttttgcgacg cgcttttgca tgacctttgc tgcgtgatgg
    27841 tgaagatggt ttcataatgg ttcgtctttt agccgtttta ttcagtatag atttgcggcg
    27901 gaatctaaca gaaagcaagc aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc
    27961 tgactcataa cccttaacgg gtcttacaat ccttgcaggc cgccaacaca ccctgttttt
    28021 ccattagccg gaacaatccg tgttgattcc atttccgttt aattacgtct ccggacgttt
    28081 gtcttcctga caatcctctg tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat
    28141 tgttcctgtt agcgtcctcg ctctatgggt gctactttac gcctttgctt taaacaaaca
    28201 atcaaccgcc atcagtattt gcacattttc agagttttat ataaaagcaa ttaactgatt
    28261 tatatagctt atttgacatg catcgcagaa ttttctctgc tatttcgcat aaagactatc
    28321 ggcaatgtct cacaaagcgt atggctttta cttacaacgc tcacgccgaa aaatccgcct
    28381 tttattatgg ttcaggtata atcgccgcaa agctttgact gacggagacg accgctttga
    28441 ctaatttgaa ttatcaacag acgcattttg tgatgagtgc gcctgatatt cgccacctac
    28501 cttccgatac cggaattgaa gtggcttttg caggccgttc caacgcaggt aaatccagcg
    28561 cgctgaacac gctgactaac cagaaaagcc tggctcgtac ctcaaaaacc cctggacgca
    28621 cccagcttat caacctgttt gaagtggctg acggcaagcg tctggttgac ttgcctgggt
    28681 acggttatgc ggaagtcccg gaagagatga agcgcaaatg gcagcgcgcg ctcggcgagt
    28741 acctcgaaaa acgtcagagc ctacaaggtc tggtagtgct aatggatatt cgccatccgc
    28801 tgaaagattt ggatcagcag atgattgagt gggcggtaga cagcaatatc gccgtgctgg
    28861 tactgctgac caaagcggac aaactggcaa gcggcgcacg taaagcgcaa ttgaatatgg
    28921 tgcgtgaagc ggtgctggcg tttaacggtg atgtgcaggt tgaaacgttt tcttcgctga
    28981 agaaacaagg cgtggacaag ctgcggcaga aactggatac ctggtttaac gagatgcagc
    29041 ctgtagaaga aacgcaggac ggcgaataat tttcttacct taatgcttat gccggatgtg
    29101 gcgtatccgg cccataaatt caatagatta caattctacc gttggcctga taaacatggt
    29161 gcatcaggca ttacggatct ttcctttcgc ccaataaaaa acgccccagt cattactgac
    29221 tggggcggct aaaatattca gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag
    29281 aacatcttac ctctgtaccc tacgcgacta actttactct tttttgttca gttcagaaag
    29341 cactttttgt aatttttttt cagttgttgc ataggaattt ctaatagcca tcacaaaacg
    29401 tgacagctta tgttgcttac ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc
    29461 tgatcccagt tttcgccact ccccacttcc accagcaacg gcacatccag acgggtacag
    29521 ttttccatca gttgatgaat ctgcttcgca acggcatcga catcatctct atgaacttca
    29581 aataccagtt catcgtgtac ctgcatgatc atacgtacac gcggttgctc agcctgtaac
    29641 cacgcatcaa cggcaatcat cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc
    29701 gcgttaatgg ctgcacgttc agccgctgca cgacgagcac cattgctgga tttgatatcc
    29761 ggcagataca gacggcgtcc gtccagcgtt tcaacgtagc cctgctcttt cgcctgagca
    29821 cgggtgcgtt ccatatactc cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac
    29881 ttctgcgctt ctttacgtgg aatgttcaat tgccgcgcca gaccgaaagc actcatgcca
    29941 taaatcagac caaagttgat cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc
    30001 agtggcaaac caaacacttc tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat
    30061 gcggtcagca agcctttgtc acgcgagaga tgcgccataa tgcgcagttc aatctgcgag
    30121 tagtccgctg agacaatcac ataatcctct ggcgcaataa acgcctggcg gatacgacga
    30181 ccttcttcgt tacgcaccgg aatgttttgc aggttaggat cggttgacga taaacgtccc
    30241 gttgcagtta cagcctggtg ataagaggta tgcacacgcc cggttttcgg gttgatcatc
    30301 agcggcagct tgtcggtgta agtcgatttc agcttcgcca gaccacgata ctccagaatc
    30361 acttttggca acggatagtc cagcgccagt tcttccagta cctcttccga cgttgacggc
    30421 gcgccacccg gcgttttctt cagcggttta atgccctgtt tttcaaacag aatggtttgt
    30481 aactgcttgg tggaagaaag gttaaattcc tcacctgcaa tttcatgcgc tttcttttcc
    30541 agctcagcca gacgaagggt gagctcttca gaatgattgt gcagcacttt cggatcgatc
    30601 ttcacaccgt tacgttcaat gcgtgaaagt actggcacca gcggcatttc gatattctcg
    30661 aagacgttca acggcccttt gtgtttttgc agatccggcc acattttcag atgcaactgc
    30721 aaggtgacat cagcatcttc ggcggcgtaa cgtccggcct cttcgagggc aatctggtta
    30781 aaagtcagtt gatttttgcc tttacccgca atctcttcaa aagtgatggt tttgtgcttc
    30841 aaccaacgtt ccgcgaggct gtccatatcg tgacgcccgg caacgctatt gagaatgtag
    30901 gactccagca tggtatcaaa cgcaatccca cgcagctcaa tgccgtaatt cgccagaata
    30961 ccgcgatcgt atttcaggtt ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt
    31021 tttagcaact cgagtgcacg ctcgcgagag atttgatcgg gcgcatcaag ataatcatga
    31081 gcaaccggaa tatatgccgc tacgcctggc tcgatagcaa aagaaagccc gaccaggtta
    31141 gctgagatgt tatcaaggct gtcggtttcg gtatcaaatg caaataccgg cgctttttcc
    31201 agcttcgcaa tccacgcttt cagtgtttct tcatcaagga tggtgacgta gttgtcataa
    31261 gaaatcaccg ttgccgtcac ttctggtgct tcgtctgcaa cactggtttc ctgtggcttc
    31321 gcggctggtt ttgccccttt ggcctgtaac catttgcccg cttcgacatc agcagtccag
    31381 cgtttgaact catacttttt gaacagcccc aacaactctt ccgctgccgg ttgctgcact
    31441 tccagttgtt cacaggtcag ctccagttca acgtcggttt taatcgtcgc cagctggtat
    31501 gagagataag caacttcttt gttttgctcg agcttcgctg ccattgtttt cgcgccacgg
    31561 aagctcaacc cagcaatttt ttctggctcg gcatacagcg tatccagccc gccaagacct
    31621 tgcagcaatg cctgcgcggt tttttcaccg acgcccggta cgccaggaat gttatcagag
    31681 gagtcaccca tcagcgccag gaaatcgatg atcagttctg gcggcacgcc gtacttattc
    31741 accacctctt ccggtccgag gatggtattc gtcatggtgt tgataagcgt aatatttggc
    31801 gtcaccagct gcgccatatc tttatcgcca gtgctgatca gcaccggacg cccggctttt
    31861 tcggcttcgc gcgccagagt accgataacg tcgtccgctt ctacgccaga aaccgccagc
    31921 agcggcagtc ccatcgcttt aaccatcgcg tgcaagggtt cgatttgtgc tcgcagatcg
    31981 tccggcattg gcgggcgatg tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt
    32041 cccttggcgt caaagaccac cgctgcatgc gtcggtttat attgcatgat cagactgcgc
    32101 agcatgttga ggacaccata catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg
    32161 ggaaacgcgt gatatgcgcg ataaagataa gatgaaccat ctacaaggat aagtggattt
    32221 tgggggatct gaaccataat gtccgtgcct gtttatcaga ttatgggtaa aggatgccac
    32281 agacaggata aaaacatgag tttttcaccg cttttcgtga aaatcttttg tagatcttct
    32341 ggatcgctcg caaatcgtca tgtggataac tttgtgcata aattatttac atcgctttta
    32401 atttattatc atgcttttta cagcatgatg tttgtctttg tttttcatgc agttaaaaat
    32461 aaatccatcc ctaataattg agataatggg attttttcta atgtggatag atatgaatta
    32521 tttttctcct taaggatcat ccgttatttg ggtcgttttc tgctaaaatc tgcgccttgc
    32581 gcttctttag cgcactgtct atggctaacc ttttgaataa attcattatg acgagaatac
    32641 tcgctgcgat aacccttttg ctgagtatcg tattgaccat tttggtcact attttttgtt
    32701 ccgtcccgat catcattgcc gggattgtaa aactattgct acctgtgcca gtcatttggc
    32761 gaaaggtttc gcgtttttgt gattttatga tgtattgctg gtgtgaaggt ctggcggtat
    32821 tactgcatct gaacccacac ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga
    32881 agaactggta tctgcttatt tgtaaccacc gtagttgggc agatattgtc gtactgtgcg
    32941 tgctgtttcg taagcacatt ccgatgaata aatatttcct caagcaacag ctggcctggg
    33001 tgccatttct tggcctggcg tgctggcgct ggatatgccg tttatgaagc gttattcccg
    33061 cgcttattta ttacgtcatc cggagcgacg tggtaaagat gttgaaacca ctcggcgttc
    33121 ttgtgaaaag tttcgcctgc atcccaccac cattgtcaat ttcgttgaag gctcccgttt
    33181 cacgcaagaa aaacatcagc aaacccactc cactttccaa aacttgttgc caccaaaggc
    33241 ggcaggcatt gcgatggcgc taaatgtact gggtaaacaa ttcgataaac tgcttaatgt
    33301 tacgttgtgt tatccggaca ataaccgtca gccgttcttc gatatgttaa gcggtaaact
    33361 gacgcggatt gtcgtacatg tggatttaca gcctattgcc gatgagttac atggcgacta
    33421 catcaatgac aaaagcttta agcgccattt tcagcaatgg ctgaactcat tgtggcagga
    33481 aaaagaccga cttctgacgt cgttgatgtc atcacagcgg caggacaagt aaattttgct
    33541 cgcctctgaa tgagcagagg cgagtgagta ttttaatgaa caaaacgtcc ggcacgagac
    33601 ataaattctt cttcgctcat ctcctgtccg ttaaaaacaa ctttacccgg tgtataacgc
    33661 agctgcagcg aggcggtatt gttgtcaatc gtgattaact gaaacatctg acctaatgtc
    33721 atcatcccgc tgatttgttt atcagcctgc ttttgcgctt tttccgcatc cattccttca
    33781 gataaattaa gctgttttgc ggtttctg
//
LOCUS       DAZFDC010000038        33774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFDC010000038 DAZFDC010000000
VERSION     DAZFDC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.038"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..246
                     /gene="ydjO"
                     /locus_tag="ACHJ48_001935"
                     /pseudo
     CDS             1..246
                     /gene="ydjO"
                     /locus_tag="ACHJ48_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(250..1641)
                     /gene="tcyP"
                     /locus_tag="ACHJ48_001936"
     CDS             complement(250..1641)
                     /gene="tcyP"
                     /locus_tag="ACHJ48_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO4707498.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1774..2364)
                     /gene="ydjM"
                     /locus_tag="ACHJ48_001937"
     CDS             complement(1774..2364)
                     /gene="ydjM"
                     /locus_tag="ACHJ48_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4707499.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2527..3195)
                     /gene="hxpB"
                     /locus_tag="ACHJ48_001938"
     CDS             complement(2527..3195)
                     /gene="hxpB"
                     /locus_tag="ACHJ48_001938"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO4707500.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3342..3878
                     /gene="yniB"
                     /locus_tag="ACHJ48_001939"
     CDS             3342..3878
                     /gene="yniB"
                     /locus_tag="ACHJ48_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO4707501.1"
                     /translation="MTYQQAGRIAVLKRIMGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGTRMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3919..4779)
                     /gene="yniA"
                     /locus_tag="ACHJ48_001940"
     CDS             complement(3919..4779)
                     /gene="yniA"
                     /locus_tag="ACHJ48_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO4707502.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4885..5175)
                     /gene="ghoS"
                     /locus_tag="ACHJ48_001941"
     CDS             complement(4885..5175)
                     /gene="ghoS"
                     /locus_tag="ACHJ48_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO4707503.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5276..6205)
                     /gene="pfkB"
                     /locus_tag="ACHJ48_001942"
     CDS             complement(5276..6205)
                     /gene="pfkB"
                     /locus_tag="ACHJ48_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO4707504.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6492..7250
                     /gene="ydiY"
                     /locus_tag="ACHJ48_001943"
     CDS             6492..7250
                     /gene="ydiY"
                     /locus_tag="ACHJ48_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO4707505.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7303..7410)
                     /gene="yniD"
                     /locus_tag="ACHJ48_001944"
     CDS             complement(7303..7410)
                     /gene="yniD"
                     /locus_tag="ACHJ48_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO4707506.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7585..9483)
                     /gene="espL1"
                     /locus_tag="ACHJ48_001945"
     CDS             complement(7585..9483)
                     /gene="espL1"
                     /locus_tag="ACHJ48_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFO4707507.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFAPQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENIFITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSIGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYISTL
                     GAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSMSFS
                     ADELKTYL"
     gene            10006..11934
                     /gene="thrS"
                     /locus_tag="ACHJ48_001946"
     CDS             10006..11934
                     /gene="thrS"
                     /locus_tag="ACHJ48_001946"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO4707508.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKIAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            11938..12480
                     /gene="infC"
                     /locus_tag="ACHJ48_001947"
     CDS             11938..12480
                     /gene="infC"
                     /locus_tag="ACHJ48_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO4707509.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            12577..12774
                     /gene="rpmI"
                     /locus_tag="ACHJ48_001948"
     CDS             12577..12774
                     /gene="rpmI"
                     /locus_tag="ACHJ48_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO4707510.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12827..13183
                     /gene="rplT"
                     /locus_tag="ACHJ48_001949"
     CDS             12827..13183
                     /gene="rplT"
                     /locus_tag="ACHJ48_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO4707511.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13300..13424
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13306..13350
                     /gene="pheM"
                     /locus_tag="ACHJ48_001950"
     CDS             13306..13350
                     /gene="pheM"
                     /locus_tag="ACHJ48_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO4707512.1"
                     /translation="MNAAIFRFFFYFST"
     gene            13633..14616
                     /gene="pheS"
                     /locus_tag="ACHJ48_001951"
     CDS             13633..14616
                     /gene="pheS"
                     /locus_tag="ACHJ48_001951"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO4707513.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            14631..17018
                     /gene="pheT"
                     /locus_tag="ACHJ48_001952"
     CDS             14631..17018
                     /gene="pheT"
                     /locus_tag="ACHJ48_001952"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO4707514.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKPADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17023..17322
                     /gene="ihfA"
                     /locus_tag="ACHJ48_001953"
     CDS             17023..17322
                     /gene="ihfA"
                     /locus_tag="ACHJ48_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO4707515.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17423..18403
                     /gene="btuC"
                     /locus_tag="ACHJ48_001954"
     CDS             17423..18403
                     /gene="btuC"
                     /locus_tag="ACHJ48_001954"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO4707516.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPMLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRALL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            18466..19017
                     /gene="btuE"
                     /locus_tag="ACHJ48_001955"
     CDS             18466..19017
                     /gene="btuE"
                     /locus_tag="ACHJ48_001955"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO4707517.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLISAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19017..19766
                     /gene="btuD"
                     /locus_tag="ACHJ48_001956"
     CDS             19017..19766
                     /gene="btuD"
                     /locus_tag="ACHJ48_001956"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO4707518.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     DVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            19844..20308
                     /gene="nlpC"
                     /locus_tag="ACHJ48_001957"
     CDS             19844..20308
                     /gene="nlpC"
                     /locus_tag="ACHJ48_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO4707519.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            20555..21268
                     /gene="ydiV"
                     /locus_tag="ACHJ48_001958"
     CDS             20555..21268
                     /gene="ydiV"
                     /locus_tag="ACHJ48_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO4707520.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            21331..22767
                     /gene="selO"
                     /locus_tag="ACHJ48_001959"
     CDS             21331..22767
                     /gene="selO"
                     /locus_tag="ACHJ48_001959"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO4707521.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPDKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWILQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(22771..22962)
                     /gene="hemP"
                     /locus_tag="ACHJ48_001960"
     CDS             complement(22771..22962)
                     /gene="hemP"
                     /locus_tag="ACHJ48_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO4707522.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(23094..24140)
                     /gene="aroH"
                     /locus_tag="ACHJ48_001961"
     CDS             complement(23094..24140)
                     /gene="aroH"
                     /locus_tag="ACHJ48_001961"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO4707523.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRINHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(24297..25130)
                     /gene="ppsR"
                     /locus_tag="ACHJ48_001962"
     CDS             complement(24297..25130)
                     /gene="ppsR"
                     /locus_tag="ACHJ48_001962"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO4707524.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            25463..27841
                     /gene="ppsA"
                     /locus_tag="ACHJ48_001963"
     CDS             25463..27841
                     /gene="ppsA"
                     /locus_tag="ACHJ48_001963"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO4707525.1"
                     /translation="MSNNGSSPLVLWYNQLGMKDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(27898..29544)
                     /gene="fadK"
                     /locus_tag="ACHJ48_001964"
     CDS             complement(27898..29544)
                     /gene="fadK"
                     /locus_tag="ACHJ48_001964"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO4707526.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFTPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCSCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(29601..29894)
                     /gene="ydiT"
                     /locus_tag="ACHJ48_001965"
     CDS             complement(29601..29894)
                     /gene="ydiT"
                     /locus_tag="ACHJ48_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4707527.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(29891..31180)
                     /gene="ydiS"
                     /locus_tag="ACHJ48_001966"
     CDS             complement(29891..31180)
                     /gene="ydiS"
                     /locus_tag="ACHJ48_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4707528.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDILVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLADIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(31236..32174)
                     /gene="ydiR"
                     /locus_tag="ACHJ48_001967"
     CDS             complement(31236..32174)
                     /gene="ydiR"
                     /locus_tag="ACHJ48_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO4707529.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(32194..32958)
                     /gene="ydiQ"
                     /locus_tag="ACHJ48_001968"
     CDS             complement(32194..32958)
                     /gene="ydiQ"
                     /locus_tag="ACHJ48_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO4707530.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SLLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGKGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            33274..>33774
                     /locus_tag="ACHJ48_001969"
     CDS             33274..>33774
                     /locus_tag="ACHJ48_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO4707531.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         8491 a         8079 c         8662 g         8542 t
ORIGIN      
        1 gggaatggga agtggtatac ttgaagatat tatgagtatt ccagatagtt cacttagaaa
       61 acgtctcggg tatgaggttt taagcttctc gctacaggca cattcattga gccaggaatg
      121 tattgataag ttggacattt tttcgctgac gatctgttta aatatgagtc agtatgcatc
      181 gccgcgatgg aacatttaaa aagtaaggca acggcccctt tacaaaacgg accgttgcca
      241 gcataagaat taatggtgtg ccagttcggc gtcgtcttcg ctatccagaa tggctttatc
      301 ggtttgcttc agccactggc tggtcagcgt gccagctgtc atcgagccac taacgtttag
      361 cgccgtacgg cccatgtcga taagcggttc aacggagatt aacagcgcca ccagggttac
      421 tggcaggccc atcgcaggca gcacaatcag tgcggcgaaa gttgcaccac caccgacacc
      481 ggcaacgcct gcggagctaa cggtaacaat accgaccagc gtcgcaatcc acatcgggtc
      541 cagcgggtta atgccaaccg taggcgcaac catcaccgcc agcattgccg gatacaaacc
      601 ggcgcagccg ttctgaccaa tggtcgcacc gaaagaggcg gcgaaactgg cgatggattc
      661 aggaacaccg agtcgacgcg tttgtgcttc cacattcagt gggatagacg cagcactgga
      721 acggctggta aaggcaaacg tcagcacagg ccatacctta cggaagtact tcagcggact
      781 cacgccatta atgcccagca gaatgccatg cactgcaaac ataatcagca gaccgaggta
      841 ggacgcgaca acgaaacttc ccagtttgat gatgtcttgc aggttagaac ctgcaaccac
      901 tttggtcatt agtgccagaa cgccgtaagg ggtcaactgc atgaccaggc gaactagttt
      961 catcacccag ctttgcaggg tatcgatagc ggttaagacg cgttcacctt tcggcgcatc
     1021 atccttcagc agtttcagcg cagctacacc gaggaatgcg gcaaaaatta ccacgctgat
     1081 aattgacgtc ggattggctc cggtcagatc ggcaaacggg tttttcggga taaaggacaa
     1141 gaccagctgc ggaacgctca ggtcagagac tttaccaaca tagttacttt cgatggcgtt
     1201 cagacgtgca gtttctgcac caccctgaac cagaccttca gccgtcaaac caaacaggtt
     1261 ggttaccagc acaccgacca gcgccgcaat cagcgtggta aacaaaagcg taccaatggt
     1321 cagaaaactg attttgccta actgagatgc gttatgcaga cgggcaaccg cgctcagaat
     1381 agaggcgaag actaacggca taacgatcat ttgcagcagt tgaacatagc cgttaccaac
     1441 aatgttaaac cactgtacag aatctttaag tacctggctg tcagaaccat aaatggtatg
     1501 cagggcaagg ccaaaaacca cacccatcac cagacccacc agcacttttt tcgccagact
     1561 ccactgttta tggcgggtct gagccagcgc aaacagcagt acaacgaaca ccacgatgtt
     1621 cgcaattaat ggaaagttca tccccgttct cctgatctta ttatttctat cactgcgtag
     1681 cagtcatagt gttggcgcaa gattaacaga actgtgagct ggctcttata ttcaaatgga
     1741 attgcttatt acagaaaggc gatttttgcc tttttagtat tcaacctgat gcttaataag
     1801 ccgatgaaac tggatctgca aggtattgat catttgcgat gaccaacgaa cagcgctgtt
     1861 ttcgggtaat gaatgaggca tccataccga ccagacaaat aatgccatgc agataaaacg
     1921 ttccagttgg ttaccctttt ggggaaccag gataggcaag cggaaacgcc agcggcatgg
     1981 ccagagcagg ggaacaccgg cgggtgtcag catatcggca agtatgtggc ttaaataacc
     2041 cagcaccatt ccttgtagcg catcagccgg aatgaaccag ctttcaggaa ccttaaggta
     2101 aaaggttgcc agcagcgcaa ataccgccag cagactgtgg gtaaaaccac gatgcccaaa
     2161 agcgcgggcg atcggttttg atatccactt taatcgctgc ccaagaaacg actttgggtg
     2221 atcgatgtcc ggtaacaaac acgtcaggat tgcggaaggg acaatatgcc accagtcacc
     2281 ctgtgccagc acgggcgtca gctcggcatt tttggcaaat accgcacaag caatagaaaa
     2341 gagaaggtga ccttccgccg tcatgataaa actcactaaa ctgtcgatac gtacagtata
     2401 ggatttttat acagtgcacg gaagtggtga cggtgtaact ctttctctta aattgacgat
     2461 taatccgtca gttgaatgca aattcagata aagaaccagg ggcagcgtga tcactgcccc
     2521 tggtcattaa ccgagaaggt cttttgcggt gagttctgtc agcgatgaga gtttgacgtt
     2581 tgctaataca aaacgtggat cattttgcgc ttctggcgca ggaacgacga tggaacgcat
     2641 gcgggctgct ttagaggcga tcatgccatt taccgaatcc tccagcgcta cgcaggtcag
     2701 tgggtcaacg cccagttttg ctgcgcagtc gagatatact tgcggatgcg gcttgctgta
     2761 aggcagtttt tcggccgagg cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa
     2821 cactttttcc agcatatgta gtggtgacgc ggaggccagt cccaccaata aaccttgttc
     2881 tttgcataac gcaacggctt cgcgcacgcc tggtaataat ggacgtgtct cttcaaccag
     2941 tgaaatggca cgggcaataa cccgttctac tacttcctga cggcttggcc cattccacgg
     3001 ttgccgggcg taccaaagat cgaccaccat atcgatgcgt aaacctaagg tgtccggcag
     3061 ctcgttacga cgggagatat ccacccccag gcttgccatc acatccagtt cggctcgatc
     3121 ccataaaggt tctgagtcga taagtaatcc atccatatca aaaattgcag caagaatctg
     3181 acgcggggtt gacattacaa cctctccttt agagcattat gtgtaaggta ttaagccgtg
     3241 cttatgatta agcgcctatc atatcgcctt taaagaatgg gcgaaaacgg atatctacag
     3301 gtaaactaag tgaaacgacg cgtcttaatc aaggagaact catgacgtat caacaagctg
     3361 gacgcattgc tgttttgaaa aggattatgg gttgggtgat ttttatacct gcattgatct
     3421 ctacattgat ttctttgctg aaatttatga atacccggca ggaaaaccag gaaggcatta
     3481 atgcggtcat gctcgacttt actcatgtca tgatcgatat gatgcaggcg aatacgcctt
     3541 ttttgaatct gttctggtat aactccccga cgcccaattt taatggtggc gtgaacgtta
     3601 tgttctgggt gattttcatc ctgatttttg ttggactggc gttgcaggat tccggtaccc
     3661 ggatgagtcg ccaggcgcgc ttcttgcggg aaggtgttga agatcaactc attctggaaa
     3721 aagccaaagg ggaagagggg ctaacgcgtg aacaaattga gtcacgtatt gttgttccgc
     3781 accatactat tttcctgcag tttttctcac tgtatatcct gccggttatt tgtattgctg
     3841 caggttatgt gttcttttct ctgcttgggt ttatttaatc aaaaggccgc acggagcggc
     3901 cattatcttc aacccatatc acgcagctaa taatctatcc agtgactgct gagcaataac
     3961 caaatgctga ccgccaaata atcttgcacg atttaacagc gtgtagagtt ggtaaaccgg
     4021 ttgacgttca aggaaatcgg caggtagcgg ggatactgac tgatagccgt catagatttg
     4081 tggcggctgt tcagtatgca gcggtaacat cgccaggtcg cactctcggt caccccagta
     4141 gcaggccggg tcgaaaatgt acgggccatc cggacccagc gcacagttgc cggaccataa
     4201 atcgccgtgc aacagagaag gctgcggttg atgtgaggcg agacgctgct gaatatgctc
     4261 gacgatggca tcgatattgc cgaaagcgat ccctttttct gctgccagtt ccaactgcca
     4321 gccaatccgt tgttcagcaa aaaacgttga ccagcgacgt tgccaggtgt tgggctgtgg
     4381 agttgtggag agcgagttat caaaatcgag tccaaattgt ggttggtcgc tccattgatg
     4441 taaacgcgca atttgctgac caagaataaa tgcgctatgc gcatccagcg gacgaggtgg
     4501 gagataatcc atcaccagaa aactgtagtc acggtcagcg ccaactgccc aaaccttagg
     4561 cacggtgacg gttttactac gcgacagtaa ctccagttgg tcggcttcgg cggtaaaacc
     4621 gggaagcagt tccctttcat cacatttgac gaaaaagtca tgtcctgcat agcgcaaatg
     4681 ccatgcggca tggacttctc cgccaggcag ttcattacgc agttcgattt cgccttcacc
     4741 taactgctcg ctcaaaagac gactgattgc ctgccacatg atgtctctcc catgttgtct
     4801 gccagatcat aaggttagcg caagaatgcg gtgtaaaaac acgagctaac gcacaccaca
     4861 gactgattat tgcccaaaaa ccatttattt tctgttgcta ttccattcct cccaggtcgt
     4921 gatggtgatt tcaggatctt tgccggttgc actttgggta agccccgaaa ccagttctct
     4981 aatttcttct tcactttggg tactgataag tccaaaagtg ttagtcccca gctcatggat
     5041 atttccctca tcatccgtca tggtgagcaa aaaaccgtcg cgagtcaggt aattattcaa
     5101 ctcgttaatt tcagtcaatg tatcctcatg caacatgacg gttatgacat aacggacaag
     5161 atcgccactg gccatagttc acccctttgt tatcacatga cttttgagca tagtcggaga
     5221 aacgcgttgc cgacaggttg gtgatgattc ccccaatgct gggggaatgt ttttgttagc
     5281 gggaaaggta agcgtaaatt ttttgcgtat cgtcatggga gcacagacgt gttccctgat
     5341 tgagtgtggc tgcactcccc gcagctacgc caaaacgaac catctcttca agagaggcat
     5401 tttctgccag tttcagtgtc atcgcgccga ccatgctatc accagcgcca acggtactct
     5461 ggcttttcac cggtggtggc accacctgaa tgcagttttc actatcaaca cccagcgctc
     5521 cttgtggacc cagggaaacg acaacccgtt tggccttgcc gctattaacg atttcctgcg
     5581 cggctttgcg gacatcgtct ggctgggtga gttcgcgatt caccagcgca ctgagttctt
     5641 tttggttagg cttaaccaac tcgatgttac caatcgccag tgctgcactt aacgcttcgc
     5701 cagaactgtc gatgatgcag cggatccctt ggttttgcgc agcggaaatc agttgggtta
     5761 atttttccag cttcacacct ggcggcaggc ttccgcttat gaccaggatg gccccggatt
     5821 caatttccag aacttgctct tcaagctggc gaaactcatc ttcatttaat gccgcgcctg
     5881 gcataacaaa acgatactgc tcaccgcttg cttccacatg tacgtgtaaa ttctgccggg
     5941 tccagtcttt ggcttctaca gtagcgacgg ggacattttc atccgccaac agtgaaacca
     6001 ggtgttcgcc ggtcgcgcca cccgccggga agatcgctgt ggcactgcct ccaagatggg
     6061 caatggcgcg ggcgacgttg atgccgccgc ccccgggttc gaacaccggt gcggtacagc
     6121 gcagttttcc ttcgggataa atttgcgggg taattgttgc gctatcgaga gagggcgcaa
     6181 gtgtcaacgt atagatacgt accatcattt cctcctatag gctgatttca gtctggcacc
     6241 gaatcagcgg aaagtgaaga aattaacaat atgatttatt ggagctttat ttaaaatttt
     6301 gcagataaat atatataaat aaaaatctgt tttcgccaca tgcttattca aaaaatgaaa
     6361 taagaataca ttgataagtt attcgcgcct ttttataatt cgttaccttt ccagggacgc
     6421 taatctttga tccttaagaa agtttccggg accgcgatgg tctctttttt tgttgtacgg
     6481 actcttgata aatgaagctt ttgaagacag ttcctgccat cgttatgttg gcggggggca
     6541 tgtttgcgtc actgaatgca gctgccgatg attccgtttt tactgtcatg gacgatcctg
     6601 ccagcgctaa aaaacctttc gaaggtaatc tgaacgcagg gtatcttgca caatcaggca
     6661 atacgaaaag ctcctcactt acggctgaca ccaccatgac ctggtatgga caaaccactg
     6721 cctggtcgct gtgggggaac gccagtaata cttcttctaa tgatgagcgt tcttcagaaa
     6781 aatatgcggc gggcgggcgt agccgtttta atttaactga ttatgactat ttatttggtc
     6841 aggcaagctg gctgacagac cgttataacg gctatcgaga gcgcgatgtg ttgaccgcgg
     6901 gttatggtcg tcagtttttg aatggcccgg tacacagttt ccgctttgaa ttcggtccag
     6961 gtgtgcgtta cgacaaatat accgacaatg ccagtgaaac tcagccgctg ggctatgcct
     7021 ccggtgctta tgcatggcag cttactgaca acgctaaatt tacgcagggt gtttccgtct
     7081 ttggcgcgga agacacaacg ctgaactccg aaagcgcatt aaatgttgct attaatgagc
     7141 attttggact gaaggtggct tacaacgtca catggaactc tgaacctcca gagtctgcac
     7201 cggaacatac cgaccgtcgg acaaccttat cgctgggcta cagcatgtaa aattatctgc
     7261 cggaccgtgt tatgcggtct ggctgtcttc gtttgtcact gctcagcccc aaatcattgc
     7321 tgcccaacgt agcaacaaca tagcgccaca gattgccagt agcaccacca ccatcttcca
     7381 gtgtttttta tcaaagcgtt tagtcggcat accagtgtcc tgcatatctc ttgtgcgtga
     7441 taacggagtg taacaaaagg gacaggcatc aagggatttt ttgttattac agccgtcggg
     7501 tggcgttgta cacctgatca ctgtttctgc gttttatgaa aaaataaaat tatgtatcga
     7561 tatataaata tcgtaaatag ctggttataa atacgtcttt aattcatcag cactaaaact
     7621 catgctttgg ctgattacat ttatagcagc ataatatgtt tctacagttt tataatgatt
     7681 atgatgaatg gctatatgaa cagctgacat gttgtcatga tttttagcag ccaacagtgt
     7741 aaataactga tgttgactaa aagaatgttt ttttgcaaaa gcacccagcg tcgatatata
     7801 agataacacg acgtcttcat tacccttgct catagcgatg tataatgcag gggttccctg
     7861 tgctgtagcg ccttttaata aatccatgat gttagctttg ctcaacttgt atttaaaggc
     7921 gatgccatta attttagaga ggaaccgtgt gacacacaaa gggtgattat tttccattgc
     7981 ggcgtataat cctggctcac catcatgtat tttcatggct aaaatactca tcaccagttt
     8041 tttgtttaaa agctcttgtg ctataggagt ggttaatgca ttcagaaaga tatcgattat
     8101 ctcatcgaat ccatacaaca tggagatata aagtccaggt gtttcgttag cacttttagt
     8161 ttgcaacaat ttaaccagat tatcttcatg aatgatgtga ttatctacca gtgactgaat
     8221 ttcttgcata aatattttca cgttgtcagt atgtccgttc atcatagcca aaaataaagc
     8281 aggtgtacca atgctattct gcgcagttat tatttcaatg atttcttttt cagggagtat
     8341 gggcaactgt ctgaataatg taataagctg ttgagtctgg cctgtggcca ttattaacaa
     8401 cattgccgat ggatgtatag gggcaaatgg cattcgtgtc gcgatacccg tgaaaatagc
     8461 ccctggaact ccctcaggta ataggggcag aatagcaatt gcacagttgc gaacatacct
     8521 atcagcccag gtctgagcat cgggacctga actgatgaac ttatcaagcg agagaaagcc
     8581 ccgtgctgtc ctgtgggtgc ctctgacggc aacgttggta tcgttcgggt cgtaaacgga
     8641 cacaacataa tttaattgcc catacgccga ctgctttatt cgtagcctta ctgacaaagt
     8701 atgattgtct atatataaaa tcgcagacat atgactgaca ttgtttgcca tcattcgaca
     8761 gaagaaaata tctaaagcga agccaaaatc cttactcgcg ataacgcgac tccctgcagg
     8821 aggatggcga agtaatgctc tgaactccgc gtcccggact aaggggacat gctgctgtat
     8881 ggattcaacg gaggagtaat gtttaatcag gttgaccttg ccttttccat ttctcaatga
     8941 atcaagtgcg tattgcaatg acagatggcg acaaaaaata gcgtcagaag gagattgcga
     9001 aaaaaatgca ctaccattga gatttactgt tggttgtata gttttttctc tatggatttt
     9061 tttagacagt ttcatacaat ggttacgatt ttcggcagtt aactgttcct gttcaatata
     9121 atccagtatg cttcgcaaat ggttagcttt aataggcgtg gtttccagta ataaggaaat
     9181 gatttctttt ttatagtgta atgtaatgaa tatattttct gcatggatgg gtgaagtggc
     9241 aaagttaatg gtaaaaaggg cagtggaata acaattttgt gatatttttc gacagtttgc
     9301 ggtaaattcc tgagtaacac agtatgacag catcgaactt aagtcagagg tatcaagtag
     9361 ggtgatgagt tggggcgcaa attgattaat aggctctgat gaaggcgttg gtatgtcgct
     9421 atcgatgtgc attctatgag gagacttata atgagttctg ataataatgt cgttttgact
     9481 cataatctat tcgccttata tatgtctgga ggttttgagt atgatgatta attattatcg
     9541 ccagaacgaa atgtgcacga aaaattgcta taagaattgt gtcactttta tcattgtgat
     9601 atgaccactt cgtcattaat cttatgatcg tttaaatagt accgccaaat cacgcaacga
     9661 actttgctgg attcagttta tgctgctgta aattcgctcg cagtaaacct ttcagacgct
     9721 ccgtgatgtt atcagttgtt ctttaagcgt tttgctggtg tactcactac aaacgaattg
     9781 cgaatcgaat caatgtgaaa cggaaaggta caatctccct ttttttagtg ttaacatcgc
     9841 tcaaccgggt ggaaggtgag aggttaatgt ctttcatttg tgtacctgat tgcgaaccaa
     9901 tttagcattt gttggctaga tggtttcgca atgaactgtt aataaacaaa tttttctttg
     9961 tatgtgatct ttcgtgtggg tcaccactgc aaataaggat ataaaatgcc tgttataact
    10021 cttcctgatg gcagtcaacg ccattacgac cacgctgtaa gccccatgga tgttgcgctg
    10081 gacattggtc caggtctggc gaaagcctgt atcgcagggc gcgttaatgg cgaactggtt
    10141 gatgcttgcg atctgattga aaacgacgca caactgtcga tcattaccgc caaagacgaa
    10201 gaaggtctgg agatcattcg tcactcctgt gcgcacctgt tagggcacgc gattaaacaa
    10261 ctttggccgc ataccaaaat ggcaatcggc ccggttattg acaacggttt ttattacgac
    10321 gttgatcttg accgcacgtt aacccaggaa gatgtcgaag cactcgagaa gcggatgcat
    10381 gagcttgctg agaaaaacta cgacgtcatt aagaagaaag tcagctggca cgaagcgcgt
    10441 gaaactttcg ccaaccgtgg ggagagctac aaagtctcca ttcttgacga aaacatcgcc
    10501 catgatgaca agccaggtct gtacttccat gaagaatatg tcgatatgtg ccgcggtccg
    10561 cacgtaccga acatgcgttt ctgccatcat ttcaaactaa tgaaaacggc aggggcttac
    10621 tggcgtggcg acagcaacaa caaaatgttg caacgtattt acggtacggc gtgggcagac
    10681 aaaaaagcgc ttaacgctta cctgcagcgc ctggaagaag ccgcgaaacg cgaccaccgt
    10741 aaaatcggta aacagctcga cctgtaccat atgcaggaag aagcgccggg tatggtattc
    10801 tggcacaacg acggctggac catcttccgt gaactggaag tgtttgttcg ttctaaactg
    10861 aaagagtacc agtatcagga agttaaaggt ccgttcatga tggaccgtgt cctgtgggaa
    10921 aaaaccggtc actgggacaa ctacaaagat gcaatgttca ccacgtcttc tgagaaccgt
    10981 gaatactgca ttaagccgat gaactgcccg ggtcacgtac aaattttcaa ccaggggctg
    11041 aagtcttatc gcgatctgcc gctgcgtatg gccgagtttg gtagctgcca ccgtaacgag
    11101 ccgtcaggtt ctctgcatgg cctgatgcgc gtgcgtggat tcacccagga tgacgcgcat
    11161 atcttctgta ctgaagaaca aattcgcgat gaagttaacg gatgtatccg tttagtctat
    11221 gatatgtaca gcacttttgg cttcgagaag atcgtcgtca aactctccac tcgtcctgaa
    11281 aagcgtattg gcagcgacga aatgtgggat cgtgctgagg cggacctggc ggttgcgctg
    11341 gaagaaaaca acatcccgtt tgaatatcaa ctgggtgaag gcgctttcta cggtccgaaa
    11401 attgaattta ccctgtatga ctgcctcgat cgtgcatggc agtgcggtac agtacagctg
    11461 gacttctcct tgccgtctcg tctgagcgcc tcctatgtgg gcgaagacaa cgagcgtaag
    11521 gtaccggtaa tgattcaccg cgcaattctg gggtcgatgg aacgtttcat cggtatcctg
    11581 accgaagaat tcgctggttt cttcccgacc tggcttgcgc cagttcaggt tgttatcatg
    11641 aatattaccg attcacagtc tgaatacgtt aacgaattga cgcaaaaact atcaaatgcg
    11701 ggcattcgtg ttaaagcaga cttgagaaat gagaagattg gctttaaaat ccgcgagcac
    11761 actttgcgtc gcgtcccgta tatgctggtc tgtggtgata aagaggtgga atcaggcaaa
    11821 attgccgttc gcacccgccg tggtaaagac ctgggaagca tggacgtaaa tgaagtgatc
    11881 gagaagctgc aacaagagat tcgcagccgc agtcttaaac aattggagga ataaggtatt
    11941 aaaggcggaa aacgagttca aacggcgcgc cctaaccgta tcaatggcga aattcgcgcc
    12001 caggaagttc gcttaacagg tctggaaggc gagcagcttg gtattgtgag tctgagagaa
    12061 gctctggaga aagcagaaga agccggagta gacttagtcg agatcagccc taacgccgag
    12121 ccgccggttt gtcgtataat ggattacggc aaattcctct atgaaaagag caagtcttct
    12181 aaggaacaga agaaaaagca aaaagttatc caggttaagg aaattaaatt ccgtcctggt
    12241 acagatgaag gcgactatca ggtaaaactc cgcagcctga ttcgctttct cgaagagggt
    12301 gataaagcca aaatcacgct gcgtttccgc ggtcgtgaga tggcgcacca gcaaatcggt
    12361 atggaagtgc ttaatcgcgt gaaagacgat ttgcaagaac tggcagtggt cgaatccttc
    12421 ccaacgaaga tcgaaggccg ccagatgatc atggtgctcg ctcctaagaa gaaacagtaa
    12481 ggccttcaag tagcactgtc tgtggagcct tcgggcttca caggctttgt tcgcctttgt
    12541 ttcgtttatt aacaatgcga agtggaagtt attaaaatgc caaaaattaa gaccgtacgc
    12601 ggtgctgcta agcgcttcaa aaaaaccggt aaaggtggtt ttaagcacaa gcacgctaac
    12661 ctgcgtcaca ttctgaccaa aaaagcgacc aaacgtaaac gtcacctgcg tccgaaagcc
    12721 atggtttcca aaggcgatct gggcctggta atcgcgtgcc tgccgtacgc ataagccgtt
    12781 aacgttttta actttttaat tagaatatag atacaggaga gcacatatgg ctcgcgtaaa
    12841 acgtggtgtt attgcacgcg cacgtcacaa gaaaattttg aaacaagcta aaggctacta
    12901 cggtgcgcgt tctcgcgtat accgcgttgc cttccaggct gttatcaaag ctggtcagta
    12961 tgcttaccgt gaccgtcgtc aacgtaagcg tcagttccgt caactgtgga ttgcgcgtat
    13021 caacgcagca gcacgtcaga acggtatttc ttacagcaaa ttcatcaatg gcctgaaaaa
    13081 agcctctgtt gaaatcgacc gtaagatcct ggctgatatc gcagtattcg acaaagtagc
    13141 gttcaccgct ctggttgaaa aagcgaaagc agctctggca taagccagtt gaaagaggga
    13201 gctagtctcc ctcttttcgt ttcaacgcca tcaaaacatt gacttttatc gccgtagcct
    13261 tttcaataaa ggtcttttga agagtaccaa aaggtaacgc aagcaatgaa tgctgctatt
    13321 ttccgcttct ttttttactt tagcacctga atccaggagg ctagcgcgtg agaagagaaa
    13381 cggaaaacag cgcctgaaag cctcccagtg gaggcttttt ttgtatgcgc gttttgaaaa
    13441 ataaaaagcc agtctatcag gcgtaattgt cgcagccagt ctggatacgg ccagtgcgga
    13501 gaaaccggag cgtacttaag tacgtgagga tttcgagcac tgcccgggac caaaatggca
    13561 agtaaaatag cctgatggga taggctctaa gtccaacgaa ccagtgtcac cactgacaca
    13621 atgaggaaaa ccatgtcaca tctcgcagaa ctggttgcca gtgcgaaggc ggccattagc
    13681 caggcgtcag atgttgccgc gttagacaat gtgcgcgtcg aatatttggg taaaaaaggg
    13741 cacttaaccc ttcagatgac gaccctgcgt gagctgccgc cagaagagcg tccggcagcc
    13801 ggtgcggtta tcaacgaagc gaaagagcag gttcagcagg cgctgaatgc gcgtaaagcg
    13861 gaactggaaa gcgctgcact gaatgcgcgt ctggcggcgg aaacgattga tgtctctctg
    13921 ccaggtcgtc gcattgaaaa cggcggtctg catccggtta cccgtaccat cgaccgtatc
    13981 gaaagtttct tcggtgagct tggctttacc gtggcaaccg ggccggaaat cgaagacgat
    14041 tatcataact tcgatgctct gaacattcct ggtcaccacc cggcgcgcgc tgaccacgac
    14101 actttctggt ttgacgctac ccgcctgctg cgtacccaga cctctggcgt acagatccgc
    14161 accatgaaag cccagcagcc accgattcgt atcatcgcgc ctggccgtgt ttatcgtaac
    14221 gactacgacc agactcacac gccgatgttc catcagatgg aaggtctgat tgttgatacc
    14281 aacatcagct ttaccaacct gaaaggcacg ctgcacgact tcctgcgtaa cttctttgag
    14341 gaagatttgc agattcgctt ccgtccttcc tacttcccgt ttaccgaacc ttctgcagaa
    14401 gtggatgtca tgggtaaaaa cggtaaatgg ctggaagtac tgggctgcgg gatggtgcat
    14461 ccgaacgtgc tgcgtaacgt tggcatcgac ccggaagttt actctggttt cgccttcggg
    14521 atggggatgg agcgtctgac tatgttgcgt tacggcgtca ccgacctgcg ttcattcttc
    14581 gaaaacgatc tgcgtttcct caaacagttt aaataaggca ggaatagatt atgaaattca
    14641 gtgaactgtg gttacgcgaa tgggtgaacc cggcgattga tagcgatgcg ctggcgaatc
    14701 aaatcactat ggcgggcctg gaagttgacg gtgtagaacc ggttgccggt agcttccacg
    14761 gcgtggtcgt tggtgaagtg gttgagtgtg cgcagcatcc gaacgctgac aaactgcgtg
    14821 tgacaaaagt gaatgtcggc ggcgatcgcc tgctggacat cgtctgcggt gcgccaaact
    14881 gccgtcaggg cctgcgtgtg gcggtagcga ccattggtgc tgttctgccg ggtgatttca
    14941 aaattaaagc ggcgaaactg cgcggcgaac cgtctgaagg gatgctgtgc tccttttctg
    15001 agctgggaat ttctgacgac cataacggca ttatcgaact gcctgcagat gcgccgattg
    15061 gcactgacat ccgcgaatac ctgaaactcg atgacaacac catcgaaatc agcgtaacgc
    15121 caaaccgtgc cgactgttta ggtatcattg gtgttgcgcg tgacgttgcc gtgctgaatc
    15181 agctgccgct ggttgaaccg gaaatcgttc cggttggtgc gaccatcgac gacacgctgc
    15241 cgattacagt cgaagcgccg gaagcctgcc cgcgttatct tggccgtgtg gtaaaaggca
    15301 ttaacgttaa agcgccaact ccgctgtgga tgaaagaaaa actgcgtcgt tgcgggatcc
    15361 gttctatcga tgcagttgtt gacgtcacca actatgtgct gctcgaattg ggccagccga
    15421 tgcacgcttt cgataaagat cgcattgaag gcggcattgt ggtgcggatg gcgaaagagg
    15481 gcgaaacgct ggtgctgctc gacggtactg aagcgaagct gaatgctgac actctggtca
    15541 tcgccgacca caacaaggcg ctggcgatgg gcggcatctt cggtggcgaa cactctggcg
    15601 tgaatgacga aacacaaaac gtgctgctgg aatgcgcttt ctttagcccg ctgtctatca
    15661 ccggtcgtgc tcgtcgtcat ggcctgcata ctgatgcgtc tcaccgttat gagcgtggcg
    15721 ttgatccggc actgcagtac aaagcgatgg aacgtgcgac ccgtctgctg attgacatct
    15781 gcggtggtga ggctggtccg gtaattgata tcaccaacga agcaacgctg ccgaagcgtg
    15841 caaccatcac tttacgtcgt agcaaactgg atcgcctgat cggccatcat attgcggatg
    15901 agcaggtaac tgacattctg cgtcgtctcg gctgcgaagt gaccgaaggc aaagacgagt
    15961 ggcaggcagt tgcgccgagc tggcgtttcg acatggagat tgaagaagat ctggtcgaag
    16021 aagtcgcgcg tgtttacggc tacaacaaca tcccggatga gccggtacag gcaagcctga
    16081 ttatgggtac tcaccgtgaa gctgacctgt cgctcaagcg cgtgaaaacg ctgctcaacg
    16141 ataaaggcta tcaggaagtg atcacctata gcttcgttga tccgaaagtg cagcagatga
    16201 tccatccagg cgttgaagcc ttactgctgc caagcccgat ctctgttgaa atgtcagcaa
    16261 tgcgtctttc tctgtggacc ggcctgctgg caaccgtggt gtacaaccag aaccgtcagc
    16321 agaaccgtgt gcgcattttc gaaagcggtc tgcgtttcgt accagatact caggcaccgt
    16381 tgggcattcg tcaggatctg atgttagccg gtgtgatttg cggtaaccgt tacgaagagc
    16441 actggaacct ggcaaaagag accgttgatt tctatgattt gaaaggcgat cttgaatccg
    16501 ttctcgacct gaccggtaaa ctgaatgagg ttgagttccg tgcagaagcg aatccggcac
    16561 tgcatccggg gcaatccgca gcgatttatc tgaaaggtga acgtattggt tttgttgggg
    16621 ttgttcatcc tgaactggaa cgtaaactgg atcttaacgg tcgcactctg gtgttcgaac
    16681 tggagtggaa caagcccgca gaccgcgtgg tgcctcaggc gcgcgagatt tctcgcttcc
    16741 cggcgaaccg tcgtgacatc gcggtggtgg tcgcagaaaa cgttcccgca gcggatattt
    16801 tatccgaatg taagaaagtt ggcgtaaatc aggtagttgg cgtaaactta tttgacgtgt
    16861 accgcggtaa gggtgttgcg gaggggtata agagcctcgc cataagcctg atcctgcaag
    16921 ataccagccg tacactcgaa gaagaggaga ttgccgctac cgtcgccaaa tgtgtagagg
    16981 cattaaaaga gcgattccag gcatcattga gggattgaac ctatggcgct tacaaaagct
    17041 gaaatgtcag aatatctgtt tgataagctt gggcttagca agcgggatgc caaagaactg
    17101 gttgaactgt ttttcgaaga gatccgtcgc gctctggaaa acggcgaaca ggtgaaactc
    17161 tctggttttg gtaacttcga tctgcgtgat aagaatcaac gcccgggacg taacccgaaa
    17221 acgggcgagg atattcccat tacagcacgg cgcgtggtga ccttcagacc cgggcagaag
    17281 ttaaaaagcc gggtcgaaaa cgcttcgccc aaagacgagt aatctgatct aactaaaaag
    17341 gccgctctgc ggcctttttt cttttcactg tcgaagagtc accgtaaaat caacgccatg
    17401 acacttcagc agaacggata ccatgctgac acttgcccgc caacaacagc gacaaaatat
    17461 tcgctggtta ttatgcctgt cagttttgat gctgctggcg cttctcttaa gcctttgcgc
    17521 cggtgaacaa tggatctcgc caggtgactg gttttctcct cgtggcgaac tgttcgtctg
    17581 gcaaattcgc ctgccacgta cgctggctgt attgctggtt ggtgcggcgc tggctatatc
    17641 cggcgctgta atgcaggcgt tgtttgaaaa tcctctggca gaacctggac tacttggcgt
    17701 ctctaacggc gcaggcgtgg ggcttatcgc cgcggtattg cttgggcaag ggcaactccc
    17761 caactgggcg ctagggctgt gtgcgattgc tggggcgctt atcatcactt taatactctt
    17821 acgtttcgcc cgtcgtcatc tttcgaccag tcggttattg ctggctggcg ttgcattagg
    17881 gattatctgt agcgcactaa tgacgtgggc tatctacttt tccacctctg ttgatttacg
    17941 tcagctgatg tactggatga tgggcggttt tggcggcgta gactggcggc aaagctggct
    18001 gatgctggca ttgatcccca tgttgttgtg gatctgttgt cagtccaggc cgatgaatat
    18061 gttagcactt ggcgagatct cggcgcggca actgggttta cccctgtggt tctggcgcaa
    18121 tgtgctggtg gcagcgaccg gctggatggt tggcgtcagt gtagcgctgg cgggtgctat
    18181 cggctttatt ggtctggtga tcccacatat tcttcggttg tgtggtttaa ccgatcatcg
    18241 cgcattactt cccggctgcg cgctggcagg ggcgagcgca ttgctgctgg ccgatattgt
    18301 agcgcgcctg gcattagctg ccgcagagct gcctattggc gtggtcaccg caacgttggg
    18361 tgcgccggtg tttatctggt tattgttaaa agcaggacgt tagccgcaaa aagacggtct
    18421 atgattaaaa gctaagattt ttactgacca cacccaggag aaacgatgca agattccatt
    18481 ctgacgaccg tagtgaaaga tatcgacggt gaagtgacca cgctggagaa gtacgccggt
    18541 aatgtgctgt tgattgtcaa tgtcgcctca aagtgtggct taacgccgca atatgagcag
    18601 ttggagaata ttcagaaagc ctgggccgat cgaggttttg tggtgctggg attcccgtgc
    18661 aaccagtttc tggaacaaga accgggcagc gatgaagaga ttaaaactta ctgtaccacc
    18721 acatgggggg tgacgttccc gatgttcagt aagattgaag ttaatggcga aggacgccat
    18781 ccgctgtatc aaaaattgat ttccgcagcg ccgaccgcag tcgcgccgga agagagcgga
    18841 ttctatgccc gtatggtcag caaaggtcgt gcaccgctgt accctgatga tattttatgg
    18901 aattttgaga aatttttagt tggcagggac ggattggtca tccagcgttt ttccccggat
    18961 atgacgccgg aagatcccat tgtgatggaa agcattaaac tggcgttggc aaaataatgt
    19021 ctattgtgat gcagttacag gatgttgcgg aatctacccg cctggggccg ctttctggcg
    19081 aggttcgggc tggggagatc ctgcacctgg tggggccgaa tggcgcgggt aagagtacct
    19141 tactggcgcg aatggccgga atgaccagcg gtaagggaag cattcagttc gcggggcaac
    19201 cactggaagc atggtccgca acaaaactcg cgctgcatcg tgcctatctt tcacaacagc
    19261 agacgccgcc gtttgcaatg ccggtctggc actacctgac actgcatcag cacgataaaa
    19321 cgcgtaccga actactgaat gatgtcgcag gggcgctggc tcttgatgac aaactcggtc
    19381 gtagcaccaa tcaactttcc ggcggtgaat ggcaacgcgt acgtcttgct gcggtggtgc
    19441 tgcaaatcac accacaagcc aatcccgcag gccaattgct gcttcttgat gagccgatga
    19501 acagtcttga tgttgcgcaa caaagtgcgt tagacaaaat tctgagcgcg ctgtgtcagc
    19561 aaggactggc gattgtgatg agcagtcacg atctcaacca cacattgcgt catgcgcatc
    19621 gggcgtggtt gctaaaaggt ggaaaaatgc tggccagtgg acgcagagaa gatgtgctca
    19681 cgccgccaaa tctggcgcag gcctatggga tgaattttcg ccgtctggat atcgaaggtc
    19741 acagaatgct gatttcgacc atctgatttc attagtatct tgaaaatcgg gtaattacga
    19801 cgctaaatta atgcaagaat aaaaaacaga ggaaccgtac ggaatgcgtt tctgccttat
    19861 tttgatcaca gcactgtttc tggccgggtg tagccaccat aaagcaccgc cgccaaatgc
    19921 cagactttct gattcgatta ccgttattgc cggtttgaac gaccagctac aaagctggca
    19981 tggcacgccg tatcgttatg gtggcatgac gcggcgcggt gtggactgtt cgggatttgt
    20041 ggttgtgacg atgcgcgatc gtttcgattt gcagctgccc cgagaaacca aagaacaagc
    20101 ctctatcggc acgcaaattg ataaagacga gttgctgcct ggtgacctgg tctttttcaa
    20161 aacgggttcc ggacaaaatg gtttgcatgt aggtatttac gataccaaca accaatttat
    20221 ccacgcctct accagcaagg gagtgatgcg ttcctcactt gataatgtct attggcagaa
    20281 aaatttctgg caggcgagac gaatctagta tgtcgaaaat tggacgtcga tgatacatcg
    20341 ccgtccattt actccagaat acagtagtat tttgcagaca aaaattccgt aaatggatta
    20401 ttcgtttata atcaggataa tctaaaaaaa ggacccctga aaaagtggtt aacatggaac
    20461 aaatgaaagt tactccttct gtaattgtta tgtcttatgg cctcatccct tttagccgga
    20521 tactgaaaaa catccttcga gagggacggt taccatgaag atttttttgg agaatcttta
    20581 tcattctgat tgctatttcc tgccgataag agataaccag caagtccttg tcggcgttga
    20641 gttaattacc catttctcca gtgaagatgg cacggtgcgt atcccgacga gtcgggttat
    20701 agcgcaactt accgaggagc aacattggca attattttca gagcagttgg aattactaaa
    20761 atcatgccaa cattttttta ttcaacataa actctttgcc tggcttaatt taacgccaca
    20821 agtggctaca ttattattag agagggataa ttacgcaggc gaactattaa aatatccgtt
    20881 tattgaactg ttgattaatg agaattaccc gcatctcaat gaaggaaaag ataatcgcgg
    20941 tcttttatcc ttatcgcaag tgtatcccct tgtgttaggt aatttgggag caggcaacag
    21001 cacaatgaaa gcggtttttg atggtctttt cacccgcgta atgctggata aaagttttat
    21061 tcagcaacaa atcacgcatc gctccttcga accgtttatt cgtgcgattc aggcgcaaat
    21121 ttccccttgt tgcaattgca tcatcgcggg gggcattgat acgccagaaa tattagcaca
    21181 gattaccccc ttcgatttcc atgctctcca gggatgcctg tggcctgccg ttcccataaa
    21241 tcagataacg acgttggttc agcgataacc ctcctgtttg ctggtgttta agacgagagt
    21301 aaccgtctac actatcaaac aggaggctct atgaccctgt cttttattac ccgctggcgc
    21361 gatgaattgc cagaaaccta tacagcactt tcccctacgc ctttaaataa tgcccggctg
    21421 atttggcata ataccgaact cgctaacacg ctgagtattc catcgtcgct gtttaaaaat
    21481 ggcgcaggtg tctggggcgg cgaaacccta ctgcctggta tgtcaccact ggcgcaggtt
    21541 tacagtggtc atcagttcgg cgtctgggcg ggccaactgg gtgatgggcg cggcatttta
    21601 cttggcgaac aactgcttgc tgatggcact acaatggact ggcatctgaa aggtgctggc
    21661 ctgacgcctt attcgcgaat gggtgatgga cgggcggtat tacgttcgac gatacgagaa
    21721 agtctcgcca gtgaggcgat gcattatctg ggcattccga cgacccgcgc gttaagtatc
    21781 gtcaccagcg attcgccagt gtatcgggaa acggtggagc caggcgcgat gctgatgcgt
    21841 gtggcaccaa gtcatctgcg ctttggtcat ttcgaacatt tttactatcg ccgcgagccg
    21901 gataaggttc gtcagttggc tgactttgcc attcgtcatt actggtcaca tcttgaagat
    21961 gatgaggaca aataccgtct ctggtttaac gatgttgtcg cacgtaccgc atcgttaatt
    22021 gcccaatggc agacggtcgg ctttgcacat ggggtgatga ataccgacaa catgtcgctg
    22081 ctgggactga cgcttgatta cgggccgttt ggttttctta atgattacga acccggtttt
    22141 atttgtaatc actcggatca tcaaggacgt tacagctttg acaatcaacc tgctgtcgcg
    22201 ttgtggattt tacagcgtct ggcgcagaca ttgtcaccat ttgttgccgt agatgccctg
    22261 aatgaggccc tggacagcta tcagcaggtt ttgttgacgc attatggaca acggatgcgg
    22321 cagaaactgg gctttatgac ggagcaaaaa gaggataacg cgctactgaa tgaattattc
    22381 agtctgatgg cgcgagagcg cagcgattat acccgcacat ttcgcatgct gagtctgacc
    22441 gagcagcaca gcgcggcgtc accgctacgt gatgagttta ttgatcgtgc ggcatttgat
    22501 gactggtttg cccgttatcg agggcgtttg caacaagacg aggttagcga tagtgagcgt
    22561 cagcaactga tgcaaagcgt taaccccgct ctggtattgc gcaactggtt ggcgcaacgg
    22621 gcgattgaag cggcagaaaa gggtgatatg acggaattgc accgcctgca tgaggcgttg
    22681 cgaaatcctt tcagcgacag agatgatgac tatgtcagtc gtccacctga ctggggtaaa
    22741 cggctggaag tcagctgctc gagttaaagg ctacttggtc aacagcagct tgccagcctg
    22801 agttttacgg agcagatatt cttgcccgtc atgatcgata atcagtttgc catccggacc
    22861 taacagtgtc tggctggaaa ttcgccgaat aggctgcggg gaagcggtct tgtgattggc
    22921 atccgtttca ggcgtgagtt ttctgctatc cgtataacgc ataaaaatta ataataattg
    22981 atataacaat gattcttatt atcaatatta tggctgacca gagcaagcag taatgtgttg
    23041 agaagacaac aagttgcgca gaaatgccca tcccatcagg aatgggcacg cattcagaag
    23101 cgggtatcta ccgcagaggc tagtttttcg accaggcgtt cggtatcttc ccagcccaga
    23161 cacgggtcgg taatggattg accgtaagtg agcggctgac cgccgacgat tttttgcgtt
    23221 ccttcgcgca ggaaactttc cgccataatt ccagcaatcg ccgtagagcc attgcggatt
    23281 tgctgacaaa tatcctcaca aacttctaac tggcgacggt gctgcttctg gcagttaccg
    23341 tggctgaaat ccaccaccag atgttcaggt aaatcaaact cgtgcagcgt atcgcaggct
    23401 gcggcgatat catcggcatg ataattcggt tttttgccgc cacgcataat aatgtggcca
    23461 tacgggttgc cgctggtctg atagatggtc atctgaccat ttttgtctgg cgagaggaac
    23521 atatggctgg cgcgggctgc gcggatagca tccacagcaa tccgcgtatt gccatcggta
    23581 ccatttttaa aacctaccgg acaggagagt gccgaagcca tttcacggtg gatctgactt
    23641 tcggtagtac gtgcgccaat cgcgccccaa ctgattaaat cagcaataaa ctgaccggtc
    23701 accatatcga ggaactcagt cgcggttggg acgcccagct catttacctg taaaagtaat
    23761 ttgcgcgcca gctccagacc gtgatttatc cgatagctgc cgtttaaatc tggatcggag
    23821 attagtcctt tccagccgac aacagttcgt ggtttttcaa aataggtgcg cattacgatt
    23881 tccagccgtg actggtactg gttgcgcagc gactgcagac gggtggcgta ctccattgca
    23941 gcggtgagat cgtggatcga gcaggggcca ataatgacca acagtcgctt atcttcacca
    24001 ttgagtattt tttcaattct gcggcgggag tcggtgacat gggtggcgac gccaggcgtt
    24061 acgggatacc gtagcgcgag ttcggcgggc gttaccaggc tctcaatacg cgcagtacgg
    24121 agttcgtcag ttctgttcat tacaagtctc agaaatttgt tgcttctacg gcaggactgc
    24181 cgggaagtga tgcgcatcac gataaaccaa tcgctgctga tttcaagtgc gaggtgtgtc
    24241 acacctcgcc gggtggttag catgataaca aaaaaataag ttaatgcact agttctctag
    24301 tacattcggc gactaaggcc catgatatcg aggatcttgg tggcaatctc ttctaccgaa
    24361 taattggtac tgttaatcca cgggatctga tttttacggt acaaggcttc cacttccgcg
    24421 acttccatcc tgcactgacg aagcgaggca tagcgactgt tctcccgacg ttcctcgcga
    24481 atcgccgcca gacgttccgg gtcgatagtc aggccgaaca atttatgctg aagcggtttg
    24541 agcgacgcgg gtagcaccag attatccata tcgtcggcaa taaaggggta gtttgccgcg
    24601 cggataccaa attgcattgc cagatacaga ctggtggggg ttttaccaca gcgagaaaca
    24661 ccgagcagga tcacctgagc ctgatccaga ttgcgcaacg aaatgccgtc atcgtgggcg
    24721 agggtgtaat caatcgccgc aatgcgcgca tcatatttat tgagattatt agggttaagg
    24781 ccatgggtac gatgagcaat cggcgttgga tccagtttca tctcttgttg tagcggggca
    24841 accagcgcct gaacgatatc ctggcaaaaa ccttcacttt gcaagatgat ggcgcgaatt
    24901 tccggcaaca cgatggagta gaagaccagc ggacgcacgc ctgtctggtg ataaattgcg
    24961 tcaatctgat ccttcactgc ccgtgcacgg ctctcatttt cgacaaacgg cagcgtgatg
    25021 ctgctgatag tgacgggaaa ttgtgacatt actgcgtgtc ctaatacctc cgcagttatt
    25081 gccgtaccat cagaaatata aaaaacgtgg cgatcaacag cattatccat tttgttcttc
    25141 ccgtgatgca gacataattc tatgaaagca taaattaaaa acgcacagaa gcgtagaacg
    25201 ttatgtctgg tttataaaat gaaccttcaa ttttattttt tatgaaaaca gcatttcatt
    25261 tttatggttt cgtttatacc gatggtttat gtggaaattg tcgaaaagag cagatttgcg
    25321 caacgctggg atcagtctta acaagtaaaa aaatatattt gcttgaacga ttcaccgttt
    25381 ttttcatccg gttaaatatg caaagataaa tgcgcagaaa tgtgtttctc aaaccgttca
    25441 tttatcacaa aaggattgtt cgatgtccaa caatggctcg tcaccgctgg tgctttggta
    25501 taaccaactc ggcatgaagg atgtagacag ggttgggggc aaaaatgcct ccctgggtga
    25561 aatgattact aacctttccg gaatgggtgt ttccgttccg aatggtttcg ccacaaccgc
    25621 cgacgcgttt aaccagtttc tggaccaaag cggcgtaaac cagcgcattt atgaactgct
    25681 ggataaaacg gatattgacg atgttactca gcttgcgaaa gcgggcgcgc aaatccgcca
    25741 gtggattatc gacactccct tccagcctga gctggaaaac gccatccgcg aagcctatgc
    25801 acagctttcc gccgatgacg aaaacgcctc ttttgcggtg cgctcctccg ccaccgcaga
    25861 agatatgccg gacgcttctt ttgccggtca gcaggaaacc ttcctcaacg ttcagggttt
    25921 tgacgccgtt ctcgtggcag tgaaacatgt atttgcttct ctgtttaacg atcgcgccat
    25981 ctcttatcgt gtgcaccagg gttacgatca ccgtggtgtg gcgctctccg ccggtgttca
    26041 acggatggtg cgctctgacc tcgcatcttc tggcgtgatg ttctccattg ataccgaatc
    26101 cggctttgac caggtggtgt ttatcacttc cgcatggggc cttggtgaga tggtcgtgca
    26161 gggtgcggtt aacccggatg agttttacgt gcataaaccg acactggcgg cgaatcgccc
    26221 ggctatcgtg cgccgcacca tggggtcgaa aaaaatccgc atggtttacg cgccgaccca
    26281 ggagcatggc aagcaggtta aaatcgaaga cgtaccgcag gaacagcgtg acatcttctc
    26341 gctgaccaac gaagaagtgc aggaactggc aaaacaggcc gtacaaattg agaaacacta
    26401 cggtcgcccg atggatattg agtgggcgaa agatggccac actggtaaac tgttcattgt
    26461 gcaggcgcgt ccggaaaccg tgcgctcacg cggtcaggtc atggagcgtt atacgctgca
    26521 ttcacagggt aagattatcg ccgaaggccg tgctatcggt catcgcatcg gtgcgggtcc
    26581 ggtgaaagtc atccatgata tcagcgaaat gaaccgcatc gaacctggtg acgtgctggt
    26641 cactgacatg accgacccgg actgggaacc gatcatgaag aaagcatctg ccatcgtcac
    26701 caaccgtggc ggtcgtacct gtcacgcggc gatcatcgct cgtgaactgg gcattccggc
    26761 ggtagtgggc tgtggtgatg caacagaacg gatgaaagac ggtgagaacg tcactgtttc
    26821 ttgtgccgaa ggtgataccg gttacgtcta tgcggagttg ctggaattta gcgtgaaaag
    26881 ctccagcgta gaaacgatgc cggatctgcc gttgaaagtg atgatgaacg tcggtaaccc
    26941 ggaccgtgct ttcgacttcg cctgcctgcc gaacgaaggc gtgggccttg cacgtctgga
    27001 atttatcatc aaccgtatga ttggcgtcca cccacgcgca ctgcttgagt ttgacgatca
    27061 ggaaccgcag ttgcaaaacg aaatccgcga gatgatgaaa ggttttgatt ctccgcgtga
    27121 attttacgtt ggtcgtctga ctgaagggat cgcgacgctg ggtgccgcgt tttatccgaa
    27181 gcgcgtcatt gtccgtctct ctgattttaa atcgaacgaa tatgccaacc tggtcggtgg
    27241 tgagcgttat gagccagatg aagagaaccc gatgctcggc ttccgtggcg cgggacgcta
    27301 tgtttccgac agcttccgcg actgtttcgc gctggagtgt gaagcagtga aacgtgtgcg
    27361 caacgacatg gggctgacca acgttgagat catgatcccg ttcgtgcgta ccgtagatca
    27421 ggcgaaagcg gtggttgagg aactggcgcg tcaggggctg aagcgtggtg agaacgggct
    27481 gaaaatcatc atgatgtgtg aaatcccgtc caacgccttg ctggccgagc agttcctcga
    27541 atatttcgac ggcttctcaa ttggctcaaa cgacatgacg cagctggcgc tcggtctgga
    27601 tcgtgactcc ggcgtggtgt ctgaactgtt cgatgagcgc aacgatgcgg tgaaagcact
    27661 gctgtcgatg gcgattcgtg ccgcgaagaa acagggcaaa tatgtcggga tttgcggtca
    27721 gggtccgtcc gaccacgaag actttgccgc atggttgatg gaagagggga tcgatagcct
    27781 gtctctgaac ccggacaccg tggtgcaaac ctggttaagc ctggctgaac tgaagaaata
    27841 aaataaatcc ccggcggcgt tttgtcgccg ggtttatgtg atccccgaag atgaaactta
    27901 ttcaatctct tcacagacat cctgcgttaa tcgccgcata atatctttcc gtaacaaaaa
    27961 cttttgtatt ttacctgagg cagtgcgcgg tagtttttcg attaccacga tatgttcagg
    28021 atatttatat tttgcgaccc gtttacggct aaaaaaagcc accacctctt ccagcgataa
    28081 tgaatgatgc ggcgctttca gcacgacata agcgcatgat cgttcaccta aacgttcatc
    28141 gggcattgca accacacagg catcgtgaat tttaggatgc tgcaataaaa tatcttccac
    28201 ttcacggctg ctaatatttt cgccgccgcg gacaataata tcttttttgc gtccggtaat
    28261 ttttatatag ccagcctcat ccatacggca gagatcgccg ctgtaatacc agccttcttc
    28321 atccagggca cgggcggtta attcaggttc atcaaaatat cccataaaca cattggggcc
    28381 acgcgaggct tcttcacctt cgcaacctgg cggtaaggtc ttgcgtgcgt catcgaccac
    28441 tttaatctct acacctgcgg cagcgtaacc atcggtgtgc ataaagcgcg acaaaggatc
    28501 atcgagattc accaccgcat gcggcgaact ttctgtggaa ccataaacac ttaataattt
    28561 aatgccgcgc tgctggcatt cacgcgccac ttttttgggg attgtggtac cgccgcaaag
    28621 aaagaagcgc agcgctgaaa ggtctgcggg ttgtttctct agtaaattca aaagatcata
    28681 gacaaacggc gttgcgccga gcatacagga gcaacgctgc tgctcaagca gcgcgagaca
    28741 cgcatcagga gtgaaaatat ctaacaacac gctgcgagcg ccaattaaga atggtgccgt
    28801 tacgccatgc agaaagcccg ttgcgtgacc aagtggcgca ggcatcataa agacatcctg
    28861 ccaggttaga ttcagccgcg cgcaataagc ccgctcactg gcgagaatat tgttatgcgt
    28921 aagcatcacg ccctttggca gaccctcggt tccggaggta aacagcaccg cagctaattc
    28981 atcgccgtgg gtcgttatcg ccgtggtcag tggggtattg tcggcgataa tctgacttaa
    29041 tgagagggaa gaggtggcgg gagccagttt gtccacgccg acaatttgtt gtagttgtgg
    29101 aagctgattt tgcagcggca ggattaaatc taccggacgc gtttgtttaa acaacgtcgg
    29161 tgtaaagaac atttttgcct gacacttatt aagcacccat accagttctg cttcccgcca
    29221 ggaaggcaac agcggtacgg aaaccgcacc gattttcagg caggcaagat agataacggt
    29281 aaattcacac cagcctggca gttgaaatgc tatgcgatcg cctgattcaa tacccttcgc
    29341 taacatccag tttgccagac agctcgcggc gtgatcgagc gcgctatagg tgtacgatgc
    29401 tccatgatta tcgaccacgg caattttgtc tggcatcgca cgagcggtct gctgccagta
    29461 atcggccagc gaagcatcgc cccatagccc ttgctgacga tacgccgcac gacgttgttc
    29521 gttaaacgtt aatgtcactt tcattttgga ctctcgaaac agaacatcag gcccgagatg
    29581 cgggcctgtg ggatgcatga ttagccgtag cgaaaatcaa taccgaaggt gcctgcggga
    29641 tattgccact gttcgagaat agtgttaccg cacagcaccc gacaggtgcc gcactccaga
    29701 caaccggcgg aatcaaaatg aatgtttcct gcgtcatcct gcttataaag tccggcaggg
    29761 caggctttca ttaatttacg gaattcattg atatcgggat ttgccgccaa aatgatatgc
    29821 gggtggccct catcaacatg gaatttattg acgcctaatt tgatgtcgac gttaaccgta
    29881 gcgttctggc tcatagcgcg gttgctccct taatgccatc tttcagcaag ttgatcagcc
    29941 caattttctt cgcgtgtccc atgatcattt tgcgtaccgg ctggttaggc ttaccgtcaa
    30001 tggtgaacat ctcgttcatg atgtcggcta ccattcgtgg gtattggcta aacaggcgcg
    30061 ggttttccat cagcgccggg atcttgcgaa aatgctgcat atcacgcatg acgcagcttt
    30121 gttccagctc acgtttgtat tgcgccagac tgctggcgga gaaatccgcg cgttctttgg
    30181 cggcgatcac tgttgtggcg gcagcctgag ccgatgcaat ggctaaatcc atgccgcgga
    30241 ccgtaaaacc caaattcagg cagaagcctg cggcgtcacc aacgatcatc acgccatcgt
    30301 taaccagttg cggtaccatc gccagaccgc cttctggcac catatgcgcg gaatattcaa
    30361 gcagtttgcc gccgctaatc agcgggcgaa tggcggggtg ttgtttaaaa tcttccagca
    30421 tttgcggcac gcttttttgc gcatgggcga tatcagccaa tccacaaacc agccccaagg
    30481 atatggaatc cttgttggta tagagaaatc ccccgcccat caggccgtca gaaggggaac
    30541 cggcaaacag ccaggcggca ccttcctcgc ccgtaatatt aaagcgatcg ttgatctgtt
    30601 ctggtgtgag gccaataacc tctttaacac caacagcgta atgatgcgga tcggaagcgg
    30661 gaaccattcc cagcgagcgg ccaagcatcg agttaacgcc atcggctaaa atcaccacat
    30721 tggcttcgag aatatcatcc ccggcctgga ctccagtgac cttgtttcct tcacgaacca
    30781 acgcatcgac gcgaactccc gggataaact gtgcgcccgc ctgctcggct tgttccatca
    30841 accacgggtc cagacgatta cgcaatacgg tataagatgc gtgttgcgga acatctggtt
    30901 gctcgcggtg aaaatcgagg gtaacggcgc tctcttcggt taagaaggag attttctcgc
    30961 gtgtgacctt gcgttctacc ggcgctgatg ctgcaaagcc tggaatgatt gcttcaagtg
    31021 tgtgggcata aagacgcccg ccggtcatgt ttttacatcc ggcactgtcg ccgcgttcta
    31081 tcaccaggat atccagcccg gctcgcgcca tgaccagtgc ggcaacgcta ccagcaacgc
    31141 ccgcaccgac cacaatggca tcaaatttgt catccgacat gcgttatgct cccttcccgc
    31201 tgcgtttaca gcgggagatg tggttgaaag gggaattagc ggctcaactg gctaatcagg
    31261 gcagggacga ctttgtagat atcgcccacc agaccgtagt cggcatagtt gaagattggc
    31321 gcatttttat ctttattgat ggcgacaatc acttttgcgc cgttgccgcc aaccatatgc
    31381 tggatctgcc cggagatccc tagcgtcagg tagagatcgg atttcagcaa cacgccggag
    31441 acaccgatat aacgttcacg ctccatccag ttctcgcctt cggcaattgg acgtgaacag
    31501 ccgacttcag cattcagcac cgccgccagt tcgtggacca ttttcaggtc atcctgcgct
    31561 gccagaccac gaccgacgcc aaccacacgt tttgctttgc tcaggtccac gctgcttgcg
    31621 gctttcgcac ggcgttcgcg acagagaatt tcatgacgcg gagcaacgta aggtaccgtt
    31681 tctgtcgggc actgatgaga ggtatcactg gtgcacggtt cctgaacacc gggtgcaagg
    31741 gtaatgatcg ccagcgagct gttgatcttt tcctgagcga acgctaaccc gccatacatc
    31801 cggtgttcgg cgcaaatgtg accatcgaca atatccaccg ccgtggcatc gttcaccagc
    31861 gccgcattca gttgcacact taaccgtgct gccagcgctt taccacgttt cgtcgcggcc
    31921 agtagcaaca tagcggggtg tttatctttc agcagagcag caatgctttc ggcgtaattt
    31981 tcagtgcgtt gcagcgcgtc gttttgctca agaacataaa tacattttgg accataaggc
    32041 ataactgcct gcgcctggtc ggtattttgt acaatggcat acacctgttg gccccattgc
    32101 tgagcgccgc caaacagttc agcataacgt tcaggattat cgctaaagac ccagacgctg
    32161 tttaattgac tcatcgtttc ttctccgtag gcttcagttc agggctttct tcagatgttc
    32221 cgccagctcg gcaatggcct ccggcgaatc gttatcgaga atgatgtgct tacgttctgt
    32281 ttgcggcggt acgcgaatgc cggtcagttc agcaagtggt gcgctctggc tccagccaat
    32341 atcacttgcc tgccactgat ttaccggttt tttacccgcg ccgagaatgg ctttcatcga
    32401 aggaatgcgt ggcacgttaa tatcggaggt gacgcagagc acggctggaa cagagagttc
    32461 aataacttca acgtcatctt caagcgtgcg ttcaatcacc agtgtattgc cctgacgctg
    32521 aatagcactc actgcattaa tcaccggaag ttgcagaatt tctccgacca gcaagccaac
    32581 ctgctgggca taaaggtcgc cggaaccttt accaaagatc agtaaatcga agccgatctt
    32641 ttcaactgct gccgccagcg cttttgcggt atcgagaggc agtgcatgct caagttgtgc
    32701 atcctgcacc atatacaggc tgtgcggccc gcgggatagc acgtctttgc gcactttcga
    32761 gttctgcaac aatgagccgc caacggtcag cgcggctatc tcatcgtcat ctgttgcaag
    32821 caggcttgca gcttcaatgg cattgagatc gaactggctg attttggcgt cggcattgtc
    32881 gaaattcagg gtgtattctg gagtgacaac aatgtcctgt tcttcaggca ccagcttaaa
    32941 gcaggttatt attttcatga tttctcctgt gagtcgcata cggaaccatc tccgatggaa
    33001 ggtaaaaatg gcctgaaaat agtgtgcgat agcggtgcgg ggttttcgac ggagcaagct
    33061 gttttccttg ccaacaatct tgcaaaaaaa atgcgatcgc taaaacaaaa gtgcaatttg
    33121 cggcaatata gttattccat gaccgtacaa tcttgcattc agtgtaaata atcttgttta
    33181 gctctcgcta aacgcggttg tataaccgca atacacgccg tattttatcc ggcatatatt
    33241 gtttttgctt gctcatggaa tagggaggtt gtcatgtatc aacgctgttt tgataatgcc
    33301 agtgaaacgc tgtttgtcgc tggtaaaacg ccacggcttt cacgttttgc atttagcgat
    33361 gatccaaaat gggagtctgg acatcacgtt catgacaatg aaaccgagct gatttacgtc
    33421 aagaaagggg tggcaaggtt taccatcgat tcttcgttat atgtcgcgca tgcagatgat
    33481 atagtggtga tagaacgcgg caggctgcat gcggtggcct ctgacgttaa cgatccggca
    33541 acgacgtgta cttgtgcgct gtacggcttt cagtttcagg gggctgagga aaatcatcta
    33601 ctgcaaccgc attcttgtcc ggtaattgcc gcggggcagg gaaaagaagt cattaaaacc
    33661 ttatttaatg agctaagtgt gattttgccg caaagtaaaa atagccaaac atcttcgtta
    33721 tgggatgcat ttgcctatac attagcaatt ctttactacg aaaactttaa aaat
//
LOCUS       DAZFDC010000039        33589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFDC010000039 DAZFDC010000000
VERSION     DAZFDC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.039"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            255..1193
                     /gene="tdcA"
                     /locus_tag="ACHJ48_001970"
     CDS             255..1193
                     /gene="tdcA"
                     /locus_tag="ACHJ48_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO4707532.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            1292..2281
                     /gene="tdcB"
                     /locus_tag="ACHJ48_001971"
     CDS             1292..2281
                     /gene="tdcB"
                     /locus_tag="ACHJ48_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO4707533.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            2303..3634
                     /gene="tdcC"
                     /locus_tag="ACHJ48_001972"
     CDS             2303..3634
                     /gene="tdcC"
                     /locus_tag="ACHJ48_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO4707534.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            3660..4868
                     /gene="tdcD"
                     /locus_tag="ACHJ48_001973"
     CDS             3660..4868
                     /gene="tdcD"
                     /locus_tag="ACHJ48_001973"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO4707535.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSLRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            4902..7196
                     /gene="tdcE"
                     /locus_tag="ACHJ48_001974"
     CDS             4902..7196
                     /gene="tdcE"
                     /locus_tag="ACHJ48_001974"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO4707536.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDKNGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            7210..7599
                     /gene="tdcF"
                     /locus_tag="ACHJ48_001975"
     CDS             7210..7599
                     /gene="tdcF"
                     /locus_tag="ACHJ48_001975"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO4707537.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            7671..9035
                     /gene="tdcG"
                     /locus_tag="ACHJ48_001976"
     CDS             7671..9035
                     /gene="tdcG"
                     /locus_tag="ACHJ48_001976"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4707538.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCEPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            9310..10641
                     /gene="cyuP"
                     /locus_tag="ACHJ48_001977"
     CDS             9310..10641
                     /gene="cyuP"
                     /locus_tag="ACHJ48_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4707539.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFIIVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGLVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            10669..11979
                     /gene="cyuA"
                     /locus_tag="ACHJ48_001978"
     CDS             10669..11979
                     /gene="cyuA"
                     /locus_tag="ACHJ48_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO4707540.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLSVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(12113..12277)
                     /gene="yhaL"
                     /locus_tag="ACHJ48_001979"
     CDS             complement(12113..12277)
                     /gene="yhaL"
                     /locus_tag="ACHJ48_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO4707541.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12300..13001)
                     /gene="yhaK"
                     /locus_tag="ACHJ48_001980"
     CDS             complement(12300..13001)
                     /gene="yhaK"
                     /locus_tag="ACHJ48_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO4707542.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQHLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRTYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            13106..14002
                     /gene="yhaJ"
                     /locus_tag="ACHJ48_001981"
     CDS             13106..14002
                     /gene="yhaJ"
                     /locus_tag="ACHJ48_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO4707543.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFNGK"
     gene            complement(14053..14409)
                     /gene="yhaI"
                     /locus_tag="ACHJ48_001982"
     CDS             complement(14053..14409)
                     /gene="yhaI"
                     /locus_tag="ACHJ48_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4707544.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(14650..15015)
                     /gene="yhaH"
                     /locus_tag="ACHJ48_001983"
     CDS             complement(14650..15015)
                     /gene="yhaH"
                     /locus_tag="ACHJ48_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO4707545.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(15307..16293)
                     /gene="yqjG"
                     /locus_tag="ACHJ48_001984"
     CDS             complement(15307..16293)
                     /gene="yqjG"
                     /locus_tag="ACHJ48_001984"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO4707546.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTRIISIGPWQDLDEPHGRDVRLG"
     gene            complement(16363..16755)
                     /gene="yqjF"
                     /locus_tag="ACHJ48_001985"
     CDS             complement(16363..16755)
                     /gene="yqjF"
                     /locus_tag="ACHJ48_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO4707547.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(16941..17240)
                     /gene="yqjK"
                     /locus_tag="ACHJ48_001986"
     CDS             complement(16941..17240)
                     /gene="yqjK"
                     /locus_tag="ACHJ48_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO4707548.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(17230..17634)
                     /gene="yqjE"
                     /locus_tag="ACHJ48_001987"
     CDS             complement(17230..17634)
                     /gene="yqjE"
                     /locus_tag="ACHJ48_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4707549.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(17637..17942)
                     /gene="yqjD"
                     /locus_tag="ACHJ48_001988"
     CDS             complement(17637..17942)
                     /gene="yqjD"
                     /locus_tag="ACHJ48_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO4707550.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(17980..18348)
                     /gene="yqjC"
                     /locus_tag="ACHJ48_001989"
     CDS             complement(17980..18348)
                     /gene="yqjC"
                     /locus_tag="ACHJ48_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO4707551.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(18495..18878)
                     /gene="mzrA"
                     /locus_tag="ACHJ48_001990"
     CDS             complement(18495..18878)
                     /gene="mzrA"
                     /locus_tag="ACHJ48_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO4707552.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(18882..19544)
                     /gene="yqjA"
                     /locus_tag="ACHJ48_001991"
     CDS             complement(18882..19544)
                     /gene="yqjA"
                     /locus_tag="ACHJ48_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO4707553.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(19566..19649)
                     /gene="yqiM"
                     /locus_tag="ACHJ48_001992"
     CDS             complement(19566..19649)
                     /gene="yqiM"
                     /locus_tag="ACHJ48_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO4707554.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(19889..20665)
                     /gene="exuR"
                     /locus_tag="ACHJ48_001993"
     CDS             complement(19889..20665)
                     /gene="exuR"
                     /locus_tag="ACHJ48_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO4707555.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(20795..22093)
                     /gene="exuT"
                     /locus_tag="ACHJ48_001994"
     CDS             complement(20795..22093)
                     /gene="exuT"
                     /locus_tag="ACHJ48_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO4707556.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            22576..23988
                     /gene="uxaC"
                     /locus_tag="ACHJ48_001995"
     CDS             22576..23988
                     /gene="uxaC"
                     /locus_tag="ACHJ48_001995"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO4707557.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMSGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            24003..25490
                     /gene="uxaA"
                     /locus_tag="ACHJ48_001996"
     CDS             24003..25490
                     /gene="uxaA"
                     /locus_tag="ACHJ48_001996"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO4707558.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            25573..26124
                     /gene="ygjV"
                     /locus_tag="ACHJ48_001997"
     CDS             25573..26124
                     /gene="ygjV"
                     /locus_tag="ACHJ48_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO4707559.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(26129..27373)
                     /gene="sstT"
                     /locus_tag="ACHJ48_001998"
     CDS             complement(26129..27373)
                     /gene="sstT"
                     /locus_tag="ACHJ48_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO4707560.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(27772..28737)
                     /gene="alx"
                     /locus_tag="ACHJ48_001999"
     CDS             complement(27772..28737)
                     /gene="alx"
                     /locus_tag="ACHJ48_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO4707561.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(29021..30007)
                     /gene="ygjR"
                     /locus_tag="ACHJ48_002000"
     CDS             complement(29021..30007)
                     /gene="ygjR"
                     /locus_tag="ACHJ48_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO4707562.1"
                     /translation="MIRFAVIGTNWITHQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     EFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(30086..30778)
                     /gene="ygjQ"
                     /locus_tag="ACHJ48_002001"
     CDS             complement(30086..30778)
                     /gene="ygjQ"
                     /locus_tag="ACHJ48_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO4707563.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLEKGRGKIVRLREKLARVSAVIDAKIFNRQPKYLGPSVII
                     GPFSEHGCPAQK"
     gene            complement(30855..31358)
                     /gene="ygjP"
                     /locus_tag="ACHJ48_002002"
     CDS             complement(30855..31358)
                     /gene="ygjP"
                     /locus_tag="ACHJ48_002002"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO4707564.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            31443..32579
                     /gene="rlmG"
                     /locus_tag="ACHJ48_002003"
     CDS             31443..32579
                     /gene="rlmG"
                     /locus_tag="ACHJ48_002003"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO4707565.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPQTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            32863..33177
                     /gene="higB"
                     /locus_tag="ACHJ48_002004"
     CDS             32863..33177
                     /gene="higB"
                     /locus_tag="ACHJ48_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO4707566.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            33174..>33589
                     /gene="higA"
                     /locus_tag="ACHJ48_002005"
     CDS             33174..>33589
                     /gene="higA"
                     /locus_tag="ACHJ48_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigA family
                     antitoxin"
                     /protein_id="HFO4707567.1"
                     /translation="MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         8384 a         8301 c         8695 g         8209 t
ORIGIN      
        1 cccctttttt gtattaacca cataacgaat gatgttatcg ccataaaata tggttatccc
       61 cgtcattttt tttgacaaaa atcagggttt atgctgattt ttatacttta atttattgat
      121 atttaaaggt atttaattga catgacgata ccctggaaag tattgaaagt taatttgtga
      181 gtggtcgcac atatcctgtt catttcattt tgatacactt catgccgtca atgaggtaat
      241 taacgtaggt cgttatgagc actattcttc ttccgaaaac gcagcacctg gtagtctttc
      301 aggaagtcat tagaagtggt tctatcggct cggctgcaaa agaattaggg ttaactcaac
      361 cggccgtcag taaaatcatt aacgatattg aagattattt tggtgtggaa ttagtggtgc
      421 ggaaaaatac cggagtaaca ttaacacctg ccggtcaatt gttactctct cgttccgaat
      481 ccattacccg tgaaatgaaa aatatggtta atgagataag cggtatgtct tctgaggcgg
      541 tggtggaagt ctcatttggt tttccttcat tgattggttt tacttttatg tcagggatga
      601 tcaacaagtt caaagaggtg ttcccgaaag cgcaggtttc tatgtatgaa gcgcaactgt
      661 cttcgttctt accggcaatc cgcgacggtc gacttgattt tgcgattggt acgttaagcg
      721 cagaaatgaa gcttcaggat ttacatgttg agccgctgtt cgagtccgag tttgtgctgg
      781 tagccagtaa gtcccgaaca tgcaccggca ccaccacgct ggagtcgttg aagaacgaac
      841 agtgggtgtt gccacaaact aatatggggt actacagcga actgcttact acgttacaaa
      901 gaaatggcat cagtattgaa aacatcgtta aaaccgactc agtcgtgaca atttataatc
      961 ttgttctcaa tgctgatttc ttaactgtaa ttccttgtga tatgacgtct ccttttggtt
     1021 ctaatcaatt tattactatt ccggttgaag aaacattacc tgtggcacaa tatgccgcgg
     1081 tatggtcgaa aaattatcgt attaaaaaag cagcatcggt tttggtggaa ttagccaaag
     1141 agtattcatc ttataatggg tgtagacgaa ggcaattaat agaagttggt tagttatttg
     1201 tttttattta aacataaata atccacctgt ctgtcttgca ggtgtcggtt acggttacct
     1261 acatatttaa ttcaggcgaa gaggttttat aatgcatatt acatacgatc tgccggttgc
     1321 tattgatgac attattgaag cgaaacaacg actggctggg cgaatttata aaacaggcat
     1381 gcctcgctcc aactatttta gtgaacgttg caaaggtgaa atattcctga agtttgaaaa
     1441 tatgcagcgt acgggttcat ttaaaattcg tggcgcattt aataaattaa gttcactgac
     1501 tgatgcggaa aaacgcaaag gcgtggtggc ctgttctgcg ggcaaccatg cgcaaggggt
     1561 ttcgctctcc tgcgcgatgc tgggtatcga cggtaaagtg gtgatgccaa aaggcgcgcc
     1621 gaaatccaaa gtagcggcaa cgtgcgacta ctccgcagaa gtcgttctgc atggtgataa
     1681 cttcaacgac actatcgcta aagtgagcga aattgtcgaa atggaaggcc gtatttttat
     1741 cccaccttac gatgatccga aagtgattgc tggtcaggga acgattggcc tggaaattat
     1801 ggaagatctc tatgatgtcg ataacgtgat tgtgccaatt ggtggtggcg gtttaattgc
     1861 tggtattgcg gtggcaatta aatctattaa cccgaccatt cgtgttattg gcgtacagtc
     1921 tgaaaacgtt cacggcatgg cggcttcttt ccactccgga gaaataacca cgcaccgaac
     1981 taccggcacc ctggcggatg gttgtgatgt ctcccgcccg ggtaatttaa cttacgaaat
     2041 cgttcgtgaa ttagtcgatg acatcgtgct ggtcagcgaa gacgaaatca gaaacagtat
     2101 gattgcctta attcagcgca ataaagtcgt caccgaaggc gcaggcgctc tggcatgtgc
     2161 tgcattatta agcggtaaat tagaccaata tattcaaaac agaaaaaccg tcagtattat
     2221 ttccggcggc aatatcgatc tttctcgcgt ctctcaaatc accggtttcg ttgacgctta
     2281 attaattcgt tgaggatagg atatgagtac ttcagatagc attgtatcca gccagacaaa
     2341 acaatcgtcc tggcgtaaat cagataccac atggacgtta ggcttgtttg gtacggcaat
     2401 cggcgccggg gtgctgttct tccctatccg cgcaggtttt ggcggactga tcccgattct
     2461 tctgatgttg gtattggcat accccatcgc attttattgc caccgggcgc tggcgcgtct
     2521 gtgtctttct ggctctaacc cttccggcaa cattacggaa acggtggaag agcattttgg
     2581 taaaactggc ggcgtggtta tcacgttcct gtacttcttc gcgatttgcc cactgctgtg
     2641 gatttatggc gttactatta ccaatacctt tatgacgttc tgggaaaacc agctcggctt
     2701 tgcaccgctg aatcgcggct ttgtggcgct gttcctgttg ctgctgatgg ctttcgtcat
     2761 ctggtttggt aaggatctga tggttaaagt gatgagctac ctggtatggc cgtttatcgc
     2821 cagcctggtg ctgatttctt tgtcgctgat cccttactgg aactctgcag ttatcgacca
     2881 ggttgacctc ggttcgctgt cgttaaccgg tcatgacggt atcctgatca ctgtctggct
     2941 ggggatttcc atcatggttt tctcctttaa cttctcgcca atcgtctctt ccttcgtggt
     3001 ttctaaacgt gaagagtatg agaaagactt cggtcgcgac ttcaccgaac gtaaatgttc
     3061 ccaaatcatt tctcgtgcca gcatgctgat ggttgcagtg gtgatgttct ttgcctttag
     3121 ctgcctgttt acgctgtctc cggccaacat ggcggaagcc aaagcgcaga atattccagt
     3181 gctttcttat ctggctaacc actttgcgtc catgaccggt accaaaacaa cgttcgcgat
     3241 tacactggaa tatgcggctt ccatcatcgc actcgtggct atcttcaaat ctttcttcgg
     3301 tcactatctg ggaacgctgg aaggtctgaa tggcctggtc ctgaagtttg gttataaagg
     3361 cgacaaaact aaagtatcgc tgggtaaact gaacactatc agcatgatct tcatcatggg
     3421 ctccacctgg gttgttgcct acgccaaccc gaacatcctc gacctgattg aagccatggg
     3481 cgcaccgatt atcgcatccc tgctgtgcct gttgccgatg tatgccatcc gtaaagcgcc
     3541 gtctctggcg aaataccgtg gtcgtctgga taacgtgttt gttaccgtga ttggtctgct
     3601 gaccatcctg aacatcgtat ataaactgtt ttaatccgta actcaggatg agaaaagaga
     3661 tgaatgaatt tccggttgtt ttggttatta actgtggttc gtcttcgatt aagttttccg
     3721 tactcgatgc cagcgactgt gaagtattaa tgtcaggtat tgccgacggt attaactcgg
     3781 aaaatgcatt cttatccgta aatgggggag agccagcacc gctggctcac cacagctacg
     3841 aaggtgcatt gaaggcaatt gcatttgaac tggaaaaacg gaatttaaat gacagcgtgg
     3901 ccttaattgg ccaccgcatc gctcacggcg gcagtatttt taccgagtcc gccattatta
     3961 ccgatgaagt cattgataat atccgtcgcg tttctccact ggcacccctg cataattacg
     4021 ccaatttaag tggtattgaa tcggcgcagc aattatttcc gggcgtaact caggtggcgg
     4081 tatttgatac cagtttccac cagacgatgg ctccggaagc ttatttatac ggcctgccgt
     4141 ggaaatatta tgaagagtta ggtgtacgcc gttatggttt ccacggcacg tcgcaccgct
     4201 atgtttccct gcgcgcacat tctttgctga atctggcgga agatgactcc ggcctggttg
     4261 tggcgcatct tggcaatggc gcgtcaatct gcgcagttcg caacggtcag agtgttgaca
     4321 cctcaatggg aatgacgccg ctggaaggct tgatgatggg tacccgcagt ggcgatgtcg
     4381 actttggtgc gatgtcctgg gtcgccagcc aaaccaacca gagcctgggt gacctggaac
     4441 gcgtagtgaa taaagagtcg ggattattag gtatttccgg tctttcttcg gatttacgtg
     4501 ttctggaaaa agcctggcat gaaggtcacg aacgcgcgca gctggcaatt aaaacctttg
     4561 ttcaccgaat tgcccgtcat attgccggac acgcagcttc attacgtcgc ctggatggaa
     4621 ttatattcac cggcggaata ggagagaatt caagtttaat tcgtcgtctg gtcatggaac
     4681 atttggctgt attaggcgta gagattgata cagaaatgaa taatcgatct aactcttttg
     4741 gcgagcgaat tgtttccagt gaaaatgcgc gtgtcatttg cgccgtaatt ccgaccaacg
     4801 aagaaaaaat gattgctttg gatgccattc atttaggcaa agttaacgca cccgcagaat
     4861 ttgcataatt tagttgaagt attgtagaga gattattttt catgaaggta gatattgata
     4921 ccagcgataa gctgtacgcc gacgcatggc ttggctttaa aggtacggac tggaaaaacg
     4981 aaattaatgt ccgcgatttt attcaacata actatacacc gtatgaaggc gatgaatctt
     5041 tcctcgctga agcgacgcca gccaccacgg aattgtggga aaaggtgatg gaaggcattc
     5101 gtattgaaaa cgcaacccac gcgccggttg atttcgatac caatattgcc accacaatta
     5161 ccgctcatga tgcgggatat attaaccagc cgctggaaaa aattgttggc ctgcaaacgg
     5221 atgcgccgtt gaaacgtgcg ttacacccgt tcggtggcat taatatgatt aaaagttcat
     5281 tccacgccta tggtcgtgaa atggacagtg aatttgaata tctgtttacc gatctgcgta
     5341 aaacccataa ccagggcgta tttgatgttt actcaccgga tatgctgcgc tgccgtaaat
     5401 ctggcgtgct gaccggttta ccagatggct atggccgtgg gcgcattatc ggtgactatc
     5461 gccgcgtagc gctgtatggc atcagttatc tggtgcgtga acgcgaactg caatttgccg
     5521 atctccagtc tcgtctggaa aaaggcgagg atctggaagc caccatccgt ctgcgtgagg
     5581 agctggcaga gcatcgtcat gcgctgttgc agattcagga aatggcggcg aaatatggct
     5641 ttgatatctc tcgcccggcg cagaatgcgc aggaagcggt gcagtggctc tacttcgctt
     5701 atctggcggc agtgaaatca cagaacggcg gcgcgatgtc gctgggccgc acggcatcgt
     5761 tcctggatat ctacattgag cgcgacttta aagctggcgt actcaatgag cagcaggcac
     5821 aggaactgat cgatcacttc atcatgaaga tccgtatggt gcgcttcttg cgtacgccgg
     5881 aatttgattc gctgttctcc ggcgacccaa tctgggcgac ggaagtgatc ggcgggatgg
     5941 ggctggacgg tcgtacgctg gtgaccaaaa actccttccg ctatttgcac accctgcaca
     6001 ctatggggcc tgcaccggaa cctaacctga ccattctttg gtcggaagaa ttaccgattg
     6061 ccttcaaaaa atatgccgcg caggtgtcga tcgtcacctc ttccttgcag tatgaaaacg
     6121 acgatctgat gcgtactgac ttcaacagcg acgattacgc gattgcctgc tgcgtcagcc
     6181 caatggtgat tggtaagcaa atgcagttct ttggtgcacg cgctaacctg gcgaaaacac
     6241 tgctgtacgc aattaacggc ggggtggacg agaagctgaa gattcaggtc gggccgaaaa
     6301 cggcaccgtt gatggacgat gtgctggatt acgacaaagt gatggacagc ctcgatcact
     6361 tcatggactg gctggcggtg cagtacatca gcgcgctgaa tatcattcac tacatgcacg
     6421 acaagtacag ctacgaagct tcgctgatgg cgctgcacga tcgtgatgtc tatcgcacca
     6481 tggcgtgcgg catcgcgggc ctgtcggtgg caacggactc cctgtctgcg atcaaatatg
     6541 cccgcgtgaa accaatccgt gacaaaaacg gcctggcggt ggactttgaa atcgacggtg
     6601 aatatccgca gtacggcaac aacgacgagc gcgtagacag cattgcctgc gacctggttg
     6661 aacgttttat gaagaaaatt aaagcgctgc caacctatcg caacgccgtc cctacccagt
     6721 cgattctgac tatcacttct aacgtggtgt acggccagaa aaccggtaac acgccggacg
     6781 gtcgtcgcgc cggaacaccg ttcgcgccgg gcgctaaccc gatgcatggt cgtgaccgca
     6841 aaggtgccgt ggcctcattg acgtcggtgg cgaaactgcc gttcacctac gccaaagatg
     6901 ggatctcgta caccttctca atcgtccctg cggcgctggg caaagaagat ccagtacgta
     6961 aaaccaacct tgtcggcctg ctggatgggt atttccacca cgaagcggat gtcgaaggcg
     7021 gtcaacacct caacgtcaac gtaatgaatc gggaaatgct gctggatgcc atcgagcacc
     7081 cggaaaaata tcctaacctg acaatccgtg tctctggcta cgccgtgcgc ttcaacgcac
     7141 tgacccgtga acagcagcag gatgttattt cacgtacctt tacccaggcg ctctgacgcc
     7201 ggaggatgta tgaaaaagat tatcgaaacg caacgtgccc caggcgcaat cggcccttat
     7261 gttcaaggcg ttgatttagg cagcatggtc ttcacctccg gtcaaattcc ggtttgccca
     7321 cagaccggtg agatcccggc tgatgtgcaa gatcaggcgc gtttaagcct cgaaaacgtc
     7381 aaagcgatcg tggttgctgc cgggctgagc gtgggcgata tcatcaagat gaccgtgttc
     7441 atcaccgatc tgaatgattt tgccaccatc aacgaagtct ataagcaatt cttcgatgag
     7501 catcaggcga cctacccgac ccggagctgt gtacaggtcg cgcgtttgcc gaaagatgtg
     7561 aagctggaaa ttgaagctat cgcagtacgt agtgcgtaaa gcctcgtgag cgggacggtc
     7621 gtaaggtcgt tccgctccac ttcactgaac ggcaatccga gggtgtggat atgattagtg
     7681 cattcgatat tttcaaaatt gggattggtc cctccagttc gcataccgtg gggccaatga
     7741 atgccggaaa aagttttatt gatcggctgg aaagtagcgg cttattaacc gcgacgagcc
     7801 atattgtggt cgatctgtac gggtcgttgt cactgacggg caaaggccat gccacggatg
     7861 tcgccatcat catgggactg gcaggaaaca gtccgcagga tgttgtcatt gatgagatcc
     7921 ctgcatttat agagttagta acgcgcagcg ggcggctgcc agtggcatct ggtgcgcata
     7981 ttgttgattt tcctgtagca aagaacatta tcttccatcc cgaaatgttg cctcgccatg
     8041 agaacggaat gcggatcact gcctggaagg cacaggaaga gctattaagt aaaacctatt
     8101 actctgtcgg cggcgggttt attgtcgaag aagaacactt cggcctgtcg cacgatgtcg
     8161 aaacacccgt accttacgat ttccactcag caggtgaact gctgaaaatg tgtgattaca
     8221 acggcctgtc tatttctggc ctgatgatgc acaacgagct agcgctgcgc agtaaagcgg
     8281 aaattgacgc cggttttgcc cgtatctggc aagtgatgca tgacggtatt gaacgcggga
     8341 tgaacactga aggcgtgctg cctggtccgc tcaatgtgcc gcgccgtgcc gtagcgctgc
     8401 gtcgtcagct ggtttccagc gataacatct ctaacgatcc gatgaatgtc atcgactgga
     8461 tcaacatgta cgcgctggcg gttagtgaag aaaacgcagc tggcgggcgc gtggtaacgg
     8521 caccgactaa cggtgcgtgc ggcattattc cggcagtgct ggcttattac gataagttcc
     8581 gtcgtccggt aaacgagcgg tcaattgccc gctattttct tgcagccggg gctattggcg
     8641 cgctgtataa aatgaacgcc tccatctctg gcgcggaggt cggctgtcag ggggagattg
     8701 gcgtggcctg ttcaatggcg gcggcagggt taactgaact actgggcggc agtccggcgc
     8761 aggtatgcaa tgcggcggaa atcgcgatgg agcataacct tggactgacc tgcgagccgg
     8821 ttgccggaca ggtacaaatc ccgtgcattg aacgtaatgc cattaatgcc gtgaaagcag
     8881 taaacgccgc gcggatggcg atgcgccgca cctcggcacc gcgtgtttca ctcgataaag
     8941 tgatcgagac gatgtatgaa accggcaaag atatgaacga taaataccgc gaaacatcac
     9001 gcggcggact ggccattaaa gtggtctgcg gctgacattg ctgtcagctt tcatccttgg
     9061 gtgcacaaat gtgcaccttt tttttgtgat ctgccgcaaa tagagagtga atattctctt
     9121 gcgattaatt tagtagaaaa ttcttatcca tctccgttat ttcaaagtct tgattgtgaa
     9181 gattttctct gacctcgcgc gtatcatttc tttattctaa taactcgcct gcaaaaacgt
     9241 agaccgcata tcgccttgat gcggctcgga aaaaaataat aagccctaca aaatattgag
     9301 cgagaaatta tggaaattgc atcgaataaa ggcgtcattg cagacgcttc gaccccggcg
     9361 ggtcgtgctg gaatgagtga gagcgagtgg cgagaagcga tcaaattcga cagtactgac
     9421 accggctggg tgattatgag tatcgggatg gcgattggcg cggggattgt ttttctcccg
     9481 gtgcaggtcg gtttgatggg attgtgggta tttttgctct catcggtgat tggttacccg
     9541 gcaatgtatc tgtttcagcg gttgtttatt aatacgctgg cagaatcacc agaatgtaaa
     9601 gattacccga gcgtcattag cggttattta ggtaaaaact ggggcatcct gttaggtgca
     9661 ctctatttcg taatgctggt gatttggatg ttcgtctatt ccaccgccat caccaacgat
     9721 agtgcttcct acctgcatac cttcggcgtg acggaagggt tgctgtcaga cagtcccttt
     9781 tatggtctgg tactgatttg cattctggtg gcgatctctt cacgcggcga gaaattgtta
     9841 ttcaaaattt cgactggcat ggtgctgacc aagctgctgg tggtcgcggc gctgggcgtg
     9901 tcgatggtgg gaatgtggca tctgtataac gtcggttcgc taccgccgct ggggctgctg
     9961 gtgaaaaacg ctattattac gctgccgttt accctgacgt cgattctgtt tatccagacg
    10021 ttaagcccga tggtgatctc ttatcgctcg cgggaaaaat cgattgaagt agcgcggcat
    10081 aaagcattgc gggcaatgaa tatcgcgttt ggcattttgt ttatcatcgt ctttttctac
    10141 gccgtgtcgt tcacgctggc gatgggacat gacgaagcgg taaaagccta cgagcagaat
    10201 atttccgcgc tggcgattgc cgcacagttt attagcggtg acggcgcagc gtgggtgaaa
    10261 gtggtcagcg tcattctcaa tatctttgct gtcatgactg cattctttgg cgtctattta
    10321 ggttttcgcg aagcaacgca agggatcgta atgaacatcc tgcgtcgcaa gatgcctgcc
    10381 gagaagatta acgaaaatct tgttcagcgc ggcatcatga ttttcgccat tttgctggcc
    10441 tggagcgcca tcgtactgaa cgcaccggtg ttgagcttca cctctatctg tagcccgatt
    10501 ttcggcttgg tagggtgcct gatcccggcg tggctggttt acaaagtacc ggcattgcac
    10561 aaatacaaag ggatgtctct gtacctgatt atcgtcactg gtttgttgct ttgtgtttct
    10621 ccgttcctgg cattttcttg atttacctga aattttaagg tttttaatat gtttgattcg
    10681 actttaaatc cgttatggca gcgttacatc ctcgccgttc aggaggaagt aaaaccggcg
    10741 ctgggatgta ctgaaccgat ttcactggcg ctggcggcgg cggttgctgc ggcagaactg
    10801 gaaggtccgg ttgaacgtgt agaagcctgg gtttcgccaa atctgatgaa gaacggtctg
    10861 ggcgtcaccg ttcccggcac gggaatggtg gggctgccga ttgcggcggc gctgggggcg
    10921 ttaggtggaa atgccaacgc cgggctggaa gtgctgaaag acgcaactgc gcaggcaatt
    10981 gccgatgcca aagcactgct ggcggcgggg aaagtctccg ttaagatcca ggaaccttgc
    11041 aatgaaatcc tcttctcacg cgccaaagtc tggaacggtg agaagtgggc gtgtgtcact
    11101 atcgtcggcg ggcataccaa cattgtgcat attgagacgc acgatggtgt ggtgtttacc
    11161 cagcaggcgt gtgtggcaga gggcgagcaa gagtctccgc tatcggtgct ttccagaacg
    11221 acgctggctg agatcctgaa gttcgtcaat gaagtcccgt ttgcggcgat ccgctttatt
    11281 ctcgattccg cgaagctaaa ttgtgcgtta tcgcaggaag gtttgagcgg taagtggggg
    11341 ctgcatattg gcgcgacgct ggaaaaacag tgcgagcgcg gtttgctggc gaaagatctc
    11401 tcttcatcca ttgtgattcg taccagcgcg gcatccgatg cgcgtatggg cggcgccacg
    11461 cttccggcaa tgagtaactc cggctcgggt aaccagggga tcaccgcaac aatgcccgtg
    11521 gtggtggtag cagaacactt cggagcggat gatgaacggc tggcgcgtgc gctgatgctt
    11581 tctcatttga gcgcaattta catccataac cagttaccgc gtttgtctgc gctgtgtgcc
    11641 gcaacgaccg cagcaatggg ggccgccgcc gggatggcat ggctggtgga tgggcgttat
    11701 gaaaccatct cgatggcgat cagcagtatg atcggcgatg tcagcggcat gatttgcgat
    11761 ggtgcgtcga acagctgcgc gatgaaggtt tcgaccagtg cttcggctgc gtggaaagcg
    11821 gtgttaatgg cgctggatga taccgccgtg accggcaatg aagggatcgt ggcgcatgat
    11881 gttgagcagt cgattgccaa cctgtgtgcg ttagcaagcc attcgatgca gcaaacggat
    11941 cggcagatta tcgagattat ggcgagcaag gccagataaa cgccaggttt tgggtatcgt
    12001 gccggatgcg atgctggcgc atcttatccg gcctacgggt tgtttaattc gttaatgttg
    12061 ttggattttg taggccggat aaggcgtttt cgccgcatcc ggcaacctgt ctttacgcgg
    12121 tagcttcatc ctcggctaaa cgcgtttcag catccgcgac gatggcttcc agctcgctca
    12181 acatctcttc atagccaaaa tttttggcgg tgcgagcagg ttctttcgcc accacgggct
    12241 tcactggctg gcgctttttg gccgattttt tactcatcat catctccctg taaaaacaac
    12301 tagacaggca aatctatcag caaagcgcgc agtggggaat cggcaaccag cgtaatgtta
    12361 gcctcgtcac gaataaacgc cccatcaccg caggtcagcg ccgctttctc ttcatgatgc
    12421 gtaagcgcat gaaatttccc gtgaatcgat tgcaaatacg tgcgtggccc atgcaactgg
    12481 aaattcgcac tttcgccttt gtcgagcacg atatggtgca gccacacttg ctggcgtaat
    12541 tgcaggcttc ccatcgcacc ctctggcgag gcgattaaat gctgcttgcc catattaagc
    12601 gccagttttt gaatcagcgg attctctcgc tgcgggcagg cgtccagcca aagctgcatt
    12661 cgtgttaacg gtttgtcttt gctgagattg tgttcgctgt agctgacacc cggctgggta
    12721 gagagcagca acgcctcacc ggcgctggcc tgaacatgat tgccttcgct gtcgcgatac
    12781 tctgcttctc catccagaat cacatttaaa atatcgactt tgggataggt tcgcggctga
    12841 aaggcggcac ctggggccag cacttcctgg ttaagcacac gcagggaggc atagcctaac
    12901 aatttcgggt cgaagtagtg tccaaaggaa aaagtatacc gggcctgcaa ccatccgtag
    12961 tctgcttgtc cacactgcct ggcagttcgg gtagtaatca taattcttga cctctcttta
    13021 cttcctttta tggtaagggg ctggacgcta cattgttagc cagatattct gcccggtatg
    13081 ttcaaatttc ctgaatgaga acgaaatggc caaagaaagg gcattaacgc tggaagcact
    13141 acgggttatg gatgcgatcg atcgccgggg cagttttgcg gcggcagcgg atgagctggg
    13201 acgcgtgcct tccgcactta gctacaccat gcaaaaactg gaagaagagc tggatgtggt
    13261 gctgtttgac cgctcgggcc atcgcaccaa attcaccaat gtcgggagga tgttgttgga
    13321 gcgagggcgc gttttgctgg aagccgcaga taaactgact actgatgcgg aagctctcgc
    13381 gcgcggttgg gaaacgcatc tcaccattgt gaccgaagcg ctggtaccga cacctgcctt
    13441 tttcccgtta atcgacaaac tggcggcaaa agccaatacc caactggcaa tcatcacaga
    13501 agtgctggcg ggggcgtggg aacggctgga gcaggggcgg gcggatattg ttatcgcgcc
    13561 ggatatgcat tttcgttcct cgtcggagat caactcgcgc aagctctata cgttaatgaa
    13621 cgtctacgtt gccgcgcctg atcacccgat tcatcaggag ccggaaccgt tatctgaagt
    13681 gacgcgtgtg aaatatcgtg gaattgcggt ggcggatacc gctcgtgagc gcccggtgtt
    13741 gactgtacag ctgctggaca aacagccgcg cttaacggtg agcacgattg aagataaacg
    13801 tcaggcatta ctggcggggc ttggcgtggc gacgatgccg tatccgatgg tcgaaaaaga
    13861 tattgcggaa gggcggttgc gtgtcgtcag cccggaatcg accagcgaga tcgatattat
    13921 tatggcctgg cgtcgtgaca gtatggggga agcgaaatcc tggtgtctgc gggaaattcc
    13981 caaacttttt aacggaaaat aatatttatt attgttaata aaaaggggcg aaatatcgcc
    14041 ccaattctaa gattaatttg aaccaaactt cggatcgttt ccgtaacgat tgctgccaga
    14101 agtaccttct gtgcagaaaa aaaccagaag cacgagccag ccaataaacg gaacaaaaaa
    14161 gagcaaggcc caggcacctg aacggtcggt atcgtgtaag cgacggatag caagcgcaag
    14221 gacgggaagg aaggttgcca gcaaataaag catagacaga tatggaagct ccagacctaa
    14281 aatcaattga atgacattga taatagcgcc gacgatggcg ttaatcagag taaacatcca
    14341 gtactcttta cgacgtgcgc gaccagagaa accaacataa ttttttaata cggataaata
    14401 ccactgcata aaaacatccc tttaattaaa agtgacgaaa gtctattccg ggtattgtgt
    14461 aaatgctatt cgtatctttc tttttcggat gttataaagg ttttgatatt taagataaat
    14521 acatggttat ttttataggt aatttatata acgacattga aatattttaa ataaacatta
    14581 gtaaattatt cattcatcaa catgatgata aaagccggat gagtcagaca tccggcgagc
    14641 agatttgttt taaggttcca gttttggatc aggtccaaag cggttttcgc ctggcgtacc
    14701 cgcctggcag ttaaagacga tgataataag ccagccgata aacgggatta agaatagcag
    14761 tgcccaccac gccgagcggt cggtgtcgtg caggcggcgg aactgaactg cccaccacgg
    14821 taaaaacacc agaataccgt agattgtcgt caggatgcct tcgccaccag cgcgttgcca
    14881 gcctaacatt ttatccagca gccccagcac gaacgtaaag atgatgttga ccagaataaa
    14941 catccagtac tctttgcgcc gcgcacgacc ccggaaaccg acataatttt ttagtacttt
    15001 cagataccag tccatttttg cctcaatttt gctcaatcac ttgtttttaa agcagcaaat
    15061 taagaataga cgtgaatgaa atggcagtaa atattcgttc aaggaattaa taaagtgctg
    15121 tgaaggcgcg ctaagactga tgacaagcgc aaaattgcca gatgcgctac gcttatctgg
    15181 cccgcgcggt cattgcaaca tattgaatat aaaagttttg taggtttgat aaggcgttca
    15241 tgccgcatcc gacatgaaca acatgcactt agctaattat cagaggcacc tcgtaaggtg
    15301 ctttttttaa cctaagcgaa catctcgtcc atgcggttca tcgagatcct gccacggacc
    15361 aattgaaata atccgcgtag ggttgatggt cttatgactg cggaagtaat gattacggat
    15421 atgatcgaaa ttgactgttt cggcaattcc cggcatctgg tagatatcac gcaggaagcc
    15481 atacagatta aggtaatcgc tgatgcggtg cttatcacac ttgaagtggg tcacatacac
    15541 tggatcaaaa cggaccagcg tggtccacag gcgaatatcg gcttcggtta gctggttgcc
    15601 ggtcaggtaa cgatgctgac ctaaaatctg ttccagtcgc gccagcgatt caaacacttt
    15661 ctccaccgct tcgtcgtagg cttgctggct ggtggcaaaa ccagctttat acacgccgtt
    15721 gttaacggtg tcataaatcc agccgttaag ttcgtcaatt ttcgtttgca gggctggtgg
    15781 gtagtaatca cccgctttcg cgcccagcgc atcaaacgcg gtattaaaca tgcggatgat
    15841 ttctgctgat tcgttgctga cgatggtgtg gttctttttg tcccacagca cgggaacagt
    15901 aactcgtccg ctgtagtgtg gatcggcgtg gagataaagc tgatacaaaa attcatgttg
    15961 atagagcgtg tctccggttg ctcccggaaa actgtcatca aaggtccagc cgttttccag
    16021 catcagcggg ttcactacgg aaacggaaat aaacggttcc agtcctttga gtttgcgcat
    16081 gatcagcgtg cggtgcgccc acgggcaggc gagtgaaaca tagagatgat aacgatcttt
    16141 ctctgcgata aaaccgcctg tgccagtggg gccaggagcg ccatcggcag tgagccagtt
    16201 acgaaatgcg gaagctgaac gttgaaattt accgccggta gatttggtat cgtaccaggt
    16261 gtcatgccag acgccgtcaa tcagttgacc cattgttctc tcctccaata gcaaaaacga
    16321 ggagcttcct cctcgttttt tatcgttaag tatagcgcgt gcttaccact ttttattcag
    16381 caggcggtcg atgctatacg cgcccggacc ggtaattgcc agcagcagga atccgccaga
    16441 aattgtcagg tttttcatga acatcagcga gttgacgcct tcagcaaagt tgctgtgaaa
    16501 taaaaatgcc gtcagcagcg taaagcccgc agtaaacagg gctgtggtgc gagtcaggaa
    16561 accgaacagg attgccagac caccaccaaa ctcaagcaga atcaccagcg gcagcataaa
    16621 acccgggacg cccatcgctt ccatatattg ttgggtaccc gcgtaaccag taatttttcc
    16681 ccagcctgcg gtaataaaca gaatcggcat taaaatgcgc gctaccagta caccaacatc
    16741 ttctaatttt ttcatcatac tctccactgc gccactcagg ctgctgattg tttttttgtg
    16801 caagggcgcg gtattagcgt cgttgctgtc gatggagaga atcataaacg tggtgaatga
    16861 tgattgttag caaggaaaac tgtcaaaaat cttcaaaaaa tttgatgggt aaggccggaa
    16921 tggctccggc cagagggaag ttaaccgcga agctgttgct gcttgagggt cgttttaacc
    16981 agacgccagg cgctccatac gccaaaaccg cgtctggccc agcggaccag catattagga
    17041 tggcgaatcg tccagatcgc catcacgcta ctgccaacca gcgcccagga gcgcagactt
    17101 agcagcatat tccagcgacg atcgtaagcg cctgttgcct ccagccattc acgacgactg
    17161 gcagaaagat ccagccgttg ttgctggatc tggctaagca gttgcgcctt acgtcgttca
    17221 cgttcgactt tactgctcac gggactcctc ctcgagcagc tgccgatcgt ttgccagctc
    17281 atggcgtgta tggcgcagca aggtggattt acgcgattta cgtaacgtcc agataccgcc
    17341 aatcagtgcc agtagcagca acaccacggt ggtggcaatc atcgcattca ggcgatattg
    17401 cggatcaacc gcccaaataa ttagcaccat caggctcata agaccaaatg cagcgaaaag
    17461 catcgtcagg cccagcatca gtaaaagttg aaagagatta gctttttcct cttccagctc
    17521 aaccaccgcc agccgcagac gtgtttccac catttcaacc atgatggaaa caattcgctg
    17581 cccgatgccc agaacgcttt taccgggccc ttgcgcgtga tgagtgtccg ccataattaa
    17641 cgacgcgaca gcagaacgcc gagcactaca ccgattgcag cgccaatgcc cacgcccgtc
    17701 catggatttt cacgcacata ctcatcggca cgcgccgccg cgacacgggt ttgtttggcg
    17761 atggcatcac cggtttcacc caggcgataa cggctctgtt tcagtgcctg ctccgctttg
    17821 ctacgaatct tactcaactc ttctttcgac ttctcgccag atgagctaag cacctcttcc
    17881 agcgtatcgg aaagggattt caactcagca cgcagatgtt ccgtagtgtg ttctttcgac
    17941 atagttttct ccaggtgagt aagtagtgac tattgtgagt tagtagtcgc gcgcttccag
    18001 ctttttcagc tcttcctgcg cttctgccag tttccgttcg cgtttggcaa tcttatcggc
    18061 atcgcctttt tgcttcgcct cggctaaatc ttgctggcgt tccgccacct catctttctg
    18121 ctttgcgatt ttcttctgat gatcggcacg cagctggcta tctgaacagt tggcccggac
    18181 ttcactcagg gctttattca gaccgtcaat acgattctgg ttttggtgtt tttcggcata
    18241 gctgatctcc ttaaggatat tttgctcctt ttcctgacac agggtagtgg cataactacc
    18301 ggcactaaga gcaaaaagag aaacagctaa agcgatgcgg tatttcattc ttgaaccttc
    18361 cattgtgatt cggacctatt acaaaggaac gattgtccag acaacgaagc ggtagtgtta
    18421 accaccgcct cattaatcat agacagttac ggtgaaatca ccaaagtgag tgatctgttt
    18481 cggacaacag gaagttatcc gaagcgatga gagttatccc gtagccgggt cagccactgc
    18541 atagcctgac tgttattgtc ctgctgcgca atgatgtagc cttggggcaa tgttctgtcg
    18601 aggaccgctt ttgcagcggc gctctggtcg ctggagtcaa aggtaattaa cagagtgtcg
    18661 tttttggggg tgatactttt gaaaggaatg ccatgagcgt caaggtgatg ccagattgaa
    18721 aaaccgtctg gcatcgttgt gccttgatga acggcacgaa tcgccagcgt agactcttgc
    18781 tggcgaaccg cggaccaggc cagcaacagc gtgccgacca gaagtaaaac agcgccggac
    18841 caggctagct ggcgaagcga catgcgaggt atttgcatcc cttacccccg atttccatat
    18901 ttctttttcc ataatacgac cagagaacct gccaggccaa acaccagcag caccaccggg
    18961 agcagcatca ggcatgacat cagctggtcc tcgtacttta aaaataccgg cgttttgccg
    19021 agcatgtaac ccagagttgt caggatcaat acccacagca gaccgctcat ccagttgaaa
    19081 aactgaaagc gcgcgttatt cagccctgat aacccggcaa tcgtcggcag cagtgttctg
    19141 acaaacgcaa taaagcgacc aattaacagc gccgataaac cgtgtttatg aaaaagatgg
    19201 tgtgcgcgtt gatgataatg cgcgggtaaa tgagatagcc agttttgtac ggtgcgggta
    19261 ttgcccagcc atcgcccctg aatatagctg acccagcagc cgaggctggc ggcaacggtc
    19321 agcagcagaa tcgtttgcgg atagcccatc gcgcctttcg caatcaacac gccgaccaat
    19381 accagtaaac tgtcgcccgg taaaaaggcc gccggaagca agccgttttc aaggaacaaa
    19441 attacaaaca agacaaaata caacatgcca atcatcgatg gattggccag ggtttcaaaa
    19501 tcctgcgccc acagggcttg cagcaattgg gtcaaaagtt ccattcatta ttcctggtga
    19561 atcggttaac gccacgctgg ttttaaaaga tgcactaccg caatgttatt gctgttttgg
    19621 tatgttctgc gggtttgata catcaacaca gcctgaacta ttccctgtta aatcgtgaga
    19681 taagaaagca ttcaaaaaca taaattcaaa ttgaatctaa ttgtaacaaa ggtcaacgtt
    19741 gttcgttatc aaaattacat gtccgtggag tctgattttg ctgaatgcag gggagggtgg
    19801 cgagaggatt tacacaggct gacactttat acttttcgct taccgacgag acaaacaggc
    19861 gcgtcgcagc gcctgtcgga caggtatttc atttactgcc actggtggcc gtatcgagat
    19921 gcactaccgg gttttcggcg aacagatagc ggtcggcatt gaactcgaag tcgtcgctgg
    19981 tttcgttgaa taacatgatc ttggtgtttt ccaggtgctg ccacattgcc agcttagcag
    20041 catgaggatc tttgcgaatc agcgccttga ggatttgatc gtggtcatca caccagttat
    20101 cgacggtacg ggaatcaatg tgttcgtgca gttttttcca gtacgggtta tggctacgct
    20161 gggtccacat tttttcaacg atagccgcca gggcggagtt ctgcgtcgcc agggctacct
    20221 gaatatggaa ctgcaaatcc cactcggaat cacggaagca ttgttcgccg cgcgcctgct
    20281 cctggatggc catcaatttc atgatgtcct gtttcgttac ctgagttgct gcgaactcgg
    20341 caatattact ttcgatgagt tggcgagcct ggagtaactc aaacggaccg taattggcga
    20401 attccatatt attgtccgca gcctgctgat ggcgcggctg gttggaaacc acgtgaatac
    20461 ctgaaccttt acgcacttcg acatagcctt caacttccag catgatgatg gcttcacgaa
    20521 ctaccgtacg gctgacgttc ttttcatcgg caataaagcg ctcagcaggc agtttatcac
    20581 ccaccagata gacgccctgt tcgatgcgct ctttcaggtc agcggcaagt tgttgataca
    20641 aacggcgtgg ttcagtgatt tccatatgcg ctccaggcat gatacggcag actatatttg
    20701 ttataccact tttgcgagtt tagctctact cgcagtgatg aaaaagccgc ctgagaaggc
    20761 ggctttgaag cgaaactaaa acttacacga agcgttaatg ttgcggtgcg ggatcgttat
    20821 gtgtttcctg cgccacctca atcgccggtt tgttctgcaa tacggtccag ataaccagcg
    20881 cacccagcag gtcaaatact gccagaactg cgaacagcgg gctgaagccg atggtgtcag
    20941 ccagtgcacc gacaaccagc gcaaacagcg tacttgccag ccatgcggac atcccggtta
    21001 aaccgttcgc cgttgccact tcgttacgac caaacacgtc ggaagagagc gtaatcagcg
    21061 cgccagacag tgcctggtgg gcaaaaccac cgatacacag cagcataatt gcgacatacg
    21121 ggttggtgaa caggccgatc atacccgggc caatcatcag cactgcaccc agcgttacga
    21181 ccatcttacg ggaaacgatc aggttcacac caaaccaacg ctggaacagc ggcggcaggt
    21241 aaccaccgag gatacaaccg aggtcagcaa acagcatcgg catccaggcg aacatcgcga
    21301 tctctttcag gttaaagccg taaactttaa acatgaacag cgggatccac gcgttaaaag
    21361 taccccaggc cggttctgcc aggaaacgcg gcagcgcgat accccagaac tgacggttac
    21421 gcaggatctg accaacggac attttcttcg ccgtactcac ctggtgctgg gcttcctgac
    21481 cattaataat atagtcgcgt tcttcatcgg tcagatgctt ctggtcgcgc ggatgtttat
    21541 agaaaatcag ccatgccatc gcccagataa agctcaatgc accggagatg ataaatgcca
    21601 tctgccagct gtgcatcacg attgcccata ccaccagcgg cggcgcaatc atcgcaccaa
    21661 tcgaagaacc tacgttaaaa tagcctactg cgatggaacg ctctttcgcc gggaaccatt
    21721 cggagctggc tttcagaccc gccgggatca tcgctgcttc cgcggcaccg accgcaccac
    21781 gagcaacagc caggccaccc cagctacctg ccagcgcggt tgcaccacag aacacggccc
    21841 acagcacagc aaacattgca taaccgattt tcgtacccag cacatccagt acataacctg
    21901 ctaccggttg catgaccgta taagcagcag aataggctgc gatgatatag gaatactgtt
    21961 gggtggagat gtttaactct tccatcagag ttggcgcagc tgccgccaca gtgttacgcg
    22021 tcaggtaacc aagcacggtg ccgagcgtca ccagtgcgat catataccaa cgtaaccctt
    22081 taattttacg catgtaaaac ctcatcgttt tgttatatcc gcaggaagcg cggccatcat
    22141 cccgccagag gctgaagatg ttagttacga ccgggtaacc gaaaaaaaac cggcacgccc
    22201 cgaacgttgc catgaaagtt tcgtgctaat tcggcttccg taccggttaa cccgattcat
    22261 ctaacaaatg aatgaatcgg caatttgcat tccgtcgact tatgatttgc gacggcagaa
    22321 agataacttg tcatacaact ttaaaaggtg agagccatca caaatgtgcg aatatttgta
    22381 gggacattac ctgacgacag cgaggccagt actggcgcgg cctgcagaga gatttaccac
    22441 tttgagagta atttttttaa ctacgtttat tgatctaact cacgaaaata tcttcggacc
    22501 ctggaaattg gtgtgataac tttgtcagca tcgcaccata agcaagctag ctcactcgtt
    22561 gagaggaaga cgaaaatgac tccgtttatg actgaagatt tcctgttaga taccgaattt
    22621 gcccgccgtc tgtatcacga ctacgcaaaa gaccagccga ttttcgatta ccattgccat
    22681 ttgccgccgc agcagattgc ggaagactat cgttttaaaa acctgtatga catctggctg
    22741 aaaggcgatc actacaaatg gcgcgctatg cgtaccaacg gtgtggccga gcgtctgtgt
    22801 accggtgatg cgtctgaccg tgaaaaattt gacgcctggg cggcgactgt tccgcacact
    22861 atcggcaacc cgttatacca ctggacgcac ctcgaactgc gtcgtccgtt tggtatcact
    22921 ggcaaattgc tttctccgtc aactgccgat gaaatctgga acgaatgtaa cgaactgctg
    22981 gcgcaggata acttctccgc gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc
    23041 accaccgatg acccgatcga ttctctggag catcacgcag agatcgccaa agacggctct
    23101 ttcaccatca aagtgctgcc gagctggcgt ccggacaaag ccttcaacat cgaacaggcg
    23161 acctttaacg actacatggc gaagctgggc gaagtttccg ataccgacat tcgccgcttt
    23221 gctgacctgc aaactgcctt gaccaaacgt ctggatcact tcgccgctca cggctgtaaa
    23281 gtgtctgacc atgcgctgga cgtggtgatg tttgctgaag cgaacgaagc ggaattggac
    23341 agcattctgg cgcgccgtct ggctggcgaa accctgagcg agcacgaagt ggcacagttc
    23401 aaaactgcgg tactggtgtt ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag
    23461 taccacattg gcgcgctgcg taataacaac ctgcgtcagt tcaaactgct ggggccggat
    23521 gtaggctttg actccatcaa cgaccgtccg atggcagaag agttgtctaa gctgctgagc
    23581 aagcagaacg aagaaaacct gctgccgaaa accattctgt actgcctgaa cccgcgcgat
    23641 aacgaagtgc tgggcaccat gagcggtaac ttccagggcg aaggtatgcc gggcaaaatg
    23701 cagttcggtt ccggctggtg gtttaacgat cagaaagacg gtatggaacg tcagatgacc
    23761 caactggcgc agctcggttt gctgagccgc tttgtcggta tgctgactga cagccgtagc
    23821 ttcctgtcat acacccgtca cgaatacttc cgccgcattc tgtgccagat gatcggtcgc
    23881 tgggtggaag cgggcgaagc accggcggac atcaacctgc tgggcgagat ggtgaaaaat
    23941 atttgcttta acaatgcgcg tgactacttc gccattgaac tgaactaagg tctggggttg
    24001 atatgcaata catcaagatc catgcgctgg ataacgtcgc ggtcgcttta gcggatttgg
    24061 ctgaaggcac agaagtcagt gtcgataacc aaactgttag gctgcgccag gatgttgctc
    24121 gtggacataa atttgcgtta acgaatatcg caaaaggggc caacgtcatc aaatatggcc
    24181 tgccgattgg ttatgcattg gcggatattg cggcggggga acacgttcac gcccacaata
    24241 cgcgcacgaa tctgagcgat ctggatcagt atcgctatca acctgatttt caggatctgc
    24301 ctgcgcaagc ggcagatcgt gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg
    24361 tgcgtaacga gctgtggatc ctgccaaccg tgggctgtgt caacggcatc gcgcggcaga
    24421 tccagaaccg ttttctgaaa gagaccaaca acgctgaagg taccgacggc gtgttcctct
    24481 tcagccacac ctacggctgc tcacagctgg gcgacgatca cattaatacc cgcaccatgc
    24541 tgcaaaacat ggtgcgccac ccgaacgcag gcgcagtgct ggtgattggt ctgggctgtg
    24601 aaaacaacca ggttgccgca ttccgtgaaa cgctgggcga tatcgatcct gaacgcgttc
    24661 atttcatgat ctgccaacag caggatgatg agatcgaagc cggaatcgag catttgcatc
    24721 agctgtataa cgtgatgcgc aacgataaac gcgagccagg caaactcagc gaactgaagt
    24781 ttggtctgga gtgcggtggt tctgacggtc tttctggtat tactgctaac ccgatgctgg
    24841 ggcgtttctc tgactacgtg attgctaacg gcggtactac cgtactgacc gaagtgccgg
    24901 agatgtttgg cgcagagcag ttgctgatgg accattgccg cgacgaagca acgtttgaaa
    24961 aactggtcac catggtcaac gacttcaaac agtactttat tgcccatgac cagccgatct
    25021 atgaaaaccc atcgccgggg aacaaagcgg gcggtatcac cacgctggaa gataaatccc
    25081 ttggctgtac ccagaaagcg ggttccagcg tcgtggttga cgtgctgcgt tacggcgagc
    25141 gtctgaaaac gccggggctg aacttgttaa gtgcgccggg taacgatgcc gtagcgacca
    25201 gcgccctggc gggtgcgggc tgccatatgg tgctgttcag tactggtcgt ggcacgccat
    25261 acggcggctt tgtaccgacg gtgaaaatcg ctaccaacag tgaactggcg gcgaagaaaa
    25321 aacactggat cgactttgac gcgggtcagc tgatccacgg taaagcgatg ccgcagttgc
    25381 tggaagaatt tatcgatacc atcgttgagt ttgccaacgg taaacaaacc tgcaacgagc
    25441 gtaacgactt ccgcgaactg gcgatcttca aaagcggcgt aacgctataa agtcgaaatg
    25501 agttgttaat aaaaaacggc gtttcataat gtgaagcgcc gtttgctttt ccatacctga
    25561 taaggattta tcatgaccgc gtattggctg gcccagggcg tgggtgtcat cgcctttctg
    25621 attggtatca caacattttt caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc
    25681 tatagcgccg ttattggcgt acattttttt cttctgggca cctatcccgc tggtgccagt
    25741 gccatcctta atgccattcg tacattgatt accttacgca cgcgcagctt atgggtaatg
    25801 gcgattttta ttgtgctgac tggcggaatt ggcctcgcga agttccatca tcctgtcgaa
    25861 ctattgccgg ttatcggtac gattgtcagt acctgggcgc tgttccgctg taaagggctc
    25921 accatgcgct gcgtgatgtg gttttcaacg tgttgctggg tgattcacaa cttctgggcg
    25981 gggtcgatag gcggcacgat gattgagggt agttttctgc ttatgaatgg cctgaatatc
    26041 attcgtttct ggcggatgca gaaaagggga attgatccgt ttaaagtaga gaaaacccct
    26101 cccgccatag acgaaagggg ttaaacaatt aattacgcag ggcgctattt gccagacgat
    26161 cgtcttccgc ctggcaagct gccgcagtga acagcacgtc agttgaagag ttcagcgcgg
    26221 tttcgcaaga gtcctgcaat acgccgataa taaagccgac ggcaaccacc tgcatggcga
    26281 tatcgttcga aataccgaac atattacagg ccagtgggat cagcagcaaa gaaccccccg
    26341 ccacgccgga tgcgccacag gcacacagag aagccactac gctcaacagc agcgctgtgg
    26401 gcagatcgac cggaataccc agcgtattaa ccgcagccag cgtcaacacg gtaatggtga
    26461 ttgctgcgcc cgccatattg atggtggctc ccagcggaat agaaacggaa taggtatcgc
    26521 gatccagatt cagcttttca cacagtgcca tattcaccgg aatgttcgcc gcagagctgc
    26581 gggtgaagaa ggcatacacg ccgctttcgc gcaggcacag cagcaccagc gggaacgggt
    26641 tacgacgaat tttccaccac accagcaatg ggttaaccac cagcgccacc agtaacatac
    26701 agccaaccag cacgaccagc agttgcgcgt agccccacag tgtggagaaa ccggtggttg
    26761 ccagggtaga agaaaccaga ccaaaaatac caatcggtgc gaagcgaatg accagtttca
    26821 ccataaaggt gacggcattc gacatatcgt tgaccaggtt tttggtggtc tcgttaccgt
    26881 gacgcagtgc gaaaccgagg ccaattgccc acaccaggat cccgatgtag ttacctttca
    26941 gcagcgcgtc gatggggttg gaaaccatgc tcattaccag cccgcgcatc acttctacaa
    27001 tgcctgacgg tggcgaaata tcacccgcgc tactggataa gtgcagggta gaagggaagg
    27061 cgaagctgaa aacgactgcg gccagagcag cagagaaggt acccagcaga tagaggaaca
    27121 aaatagggcg gatattggtt ttctgcccgt gctggtggtt agcaatagat gccatcacca
    27181 gcatcaacac caggatgggg gcaacggctt tcagtgcgcc gacgaacaaa gtacctaaca
    27241 gaccaacagc ttccgccgcg ggttttgaga tccatgccag aagaatcccc agaacaaggc
    27301 cgaccaggat ttgttttacc aggctgccat gagccagacg ccggaatagc cccggtgaac
    27361 gttgcgtagt catttttcga tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt
    27421 cgacgcatct ttcaggatgt aacaaagtgt ttgcaaggtc gagtataagg aaagttacag
    27481 cttcgggaag cgaaaaatgc tggattttaa agagaggtca tattttgcaa cttaatggtt
    27541 acatttattt gacataaaaa taaagccaac gtgcggcttc cagactgagg actacgtcaa
    27601 aaaaggactc ggtcagtccc ctcgccccgt ttggggagag ggttagggtg aggggaacag
    27661 gccagcatcg gtgcgaaaaa ttaccctcac cccgaccctc tccctggaag ggagagggag
    27721 aaaacggtgc tcatgttgat gacagggtac acggcttagg cagattaaaa atcatccaac
    27781 ccgctgctta tcatgccgat aattcaccca ggcgttgata ataaacgtca tcaccaggat
    27841 gccaaacacc acgcccagcg agacggcgat tggaatatgg tagaagtcga caatcagcat
    27901 cttgataccg ataaacacca gaatcaccgc caggccatat ttgagcatcg agaaacgctc
    27961 tgctacgccc gccagcagga aatacatcgc acgcaggccg aggatcgcaa acaggtttga
    28021 ggtcagcaca atgaacgggt cagtggtcac ggcgaagata gccggaatgc tatccacggc
    28081 aaaaatcacg tcgctcaatt ccaccagaat cagtaccagc atcagcggtg tggcgtacag
    28141 caatccattc ttacgcacaa agaaatgctc gttgtcgatg gtgtcggtca tgcgcaaatg
    28201 accgcgtagc cagcgcacca gcggcttgtc gccaatgcct gattcatctt catgggcgag
    28261 cgccatcttc acgccggtaa acagcaggaa ggcaccgaag atatacagga tccagtcgaa
    28321 ctgcgaaatc agccagctgc cagtgaagat catgatggta cgcagaacaa tcgccccgag
    28381 cacgccatac accagcacgc ggcgttgtaa tgccgccgga acggagaaat agctgaacaa
    28441 catcagccag acaaagacgt tatcgaccgc cagcgacttc tcaatcagat aacccgtgag
    28501 gaaggccagc gcctgtggat cggcaacggc gcgaccttcg gtttgtacca gataccacca
    28561 gaaagcagcg ttaaacagta acgacagcgt cacccagacc agcgaccagg ccgcagcctg
    28621 tttcatggtc atggcatgtg ccccacgacg tccctgcaac aacaggtcga tagccagcat
    28681 aatggcgaca acaacagcga atccgcccca tagcaacggc gtgccgacag tattcataga
    28741 agttccttac acataaaaaa aacggccaac gtcggaagac gtcagccgct gctttttatg
    28801 catagacctc gccttccggc aaggtctcac ttacaacaaa aaaggaatac gtctgcgtat
    28861 tcctttcggg ttgcccggtg accggatgtg gtttttcaca catcgtaatg acgatcgacc
    28921 ggcaatgaag ttactcccct ttgcaggtaa caaagtatga cagaccattc agtcagtcaa
    28981 tggtctgtta catacctttt acactgtttt actttggcaa ttatagtttt acgctatctg
    29041 ccggaaaaat caccccagtc tggcggcgga tctcggtcag cagtttggct gtgatgcgac
    29101 tgaccgccag ccctggatga tccaccagat gctcatccac cagggtagcg aacagctctg
    29161 cttcgtagag catggtatta atatgctgcg gctgggtgag atcctgcatc tggctgccac
    29221 gcggcacgaa gcacactttc tggcattcag acagtttttc aatcaccagc gatcccgctt
    29281 cgccctgaat ctcgcttgcc aggacagaat cgctgacctt tgagtgctgc aaggtgacgc
    29341 tgaaatcacc gtaatccatc accaccacgc cgtgggcatc tacaccactt gccagtaaac
    29401 tggcggttgc ctgcacgctt ttcggctcac caaataacgc taccgccgac gccaggcagt
    29461 aaaagccgat atccataatt gaaccgttag agaatgccgg attaaaggtg ttgggattct
    29521 caccgtccag gtaacgttga taccgcgagg aatactggca atagttgaaa aaaactttcc
    29581 gcagtttgcc gactttcggc agcgcctggc gtaacagatg aaagttaggc aggctggccg
    29641 ttttaaatgc ctcaaacagc accacctgat tttcacgcgc gcaggcaatg gctgcatcca
    29701 cttccgccag attcgacgcc agtggtttct cacaaatcac atgaattttg tggctcagga
    29761 aaagttgcgt ctgggaaaaa tgcagggcgt tcgggctggc aatatacacc gcgtcgatag
    29821 cgtcactttc cgccattgct tccagcgaag taaacagatg ctcgacagaa aactcattgg
    29881 cgaagtgctg ggcctgttca aggctgcggg aataaacggc ggttaactta tatttaccgc
    29941 tttcatgtgc ggcctccacg aactggtgag ttatccagtt cgtaccaatc acggcgaaac
    30001 gtatcataac gcgtggatac tccataaagg gattctgtcg tcaatgtagc acgccttttt
    30061 catcttcgac gtgcgcggat acgcattatt tctgcgccgg acaaccatgc tcactaaacg
    30121 gaccaataat gaccgacggc ccgagatatt ttggctgacg attgaagatt ttcgcgtcaa
    30181 tcaccgcgct gactctggcg agtttctctc gcagacggac aatctttccg cgtccttttt
    30241 ccaggtctgg tgcgttaaac ccgatagcat cgatgccgta ttgttttgcc agccagatgg
    30301 cgcgttggtt atgaaattcc tgcgagataa tggtgatatg gttctcccca aacactttat
    30361 tggcacgcac cactgaatca agcgttgaga atccggcata gtcacagaag atcacttttg
    30421 ccggtacgcc ctttgcgatc aaagcctgct gcatgcctga cgcttcatcg taatttttac
    30481 gtccgttatc gccactgacc agcagccatt tcacttttcc ggcgtggtac aactctgctg
    30541 cggtatcaat acgtcgggta aagtagcgat tacccggcct ggcccccagc aataagccca
    30601 cgttacgcgc cggaacgacg ttgacgtcgc tccaggtcag ctgtttgctg gcgttgacca
    30661 ttacgcgatc ggcgataaaa atggtcgctc ctgccacaag taacagtaag caggcaatct
    30721 ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg ggcaaatgcg cgcagcatgg
    30781 tcgttatcct taacgaaagg ggggaggtcg attttaggcg ctaaagaaag aaaagataac
    30841 gcctgacatc attttcagat tttgtcctgc ccaagcgaca actgggttag ccacagtcgg
    30901 gtgtcgaact caagctggtg gtactggggt tccatatgac agcacaactg gtaaaacgct
    30961 ttgttatgct ctttctcctt caggtgcgcc agctcgtgca ccacgatcat gcgcaaaaac
    31021 ggttccggcg cgttgcgaaa cacggtagcg acgcggatct ctgcttttgc tttcagcttg
    31081 ccgccctgca cacgagaaac agcggtatgt aacccgagcg cgttttttag cacatggatc
    31141 ttattgtcat acatcacttt attgatcggc ggggcattac gcagaaactg atttttcaga
    31201 tcctgagtat attgccagag ggctttatcg gtggcgtagt cgtgtgttcc cggatagcgt
    31261 tttgccagca catcgcccag acgctgttcg ttaatcagtg ttcgcacctg ggaaagtagc
    31321 tgctcgggat aaccctgaag ataagtaaga ttgctcataa ccgcccacga aaaagagaaa
    31381 aagggtatac tcacgcaccc ttttcagggg caatgtcgaa attttatcat tcaggagggc
    31441 cgatgagcca cttagacaac ggtttccgtt cactgacact acaacgtttt ccggcgacgg
    31501 atgacgttaa cccgctacag gcgtgggaag cggcggatga atatttgctg caacagttgg
    31561 acgacacaga aatccgcggc ccggtgttga tcctgaatga tgcctttggt gcgttaagct
    31621 gcgcgctggc ggaacataag ccgtacagca ttggcgactc atacatcagt gaactggcga
    31681 cgcgcgagaa tttacgcctc aacgggattg atgagtcgag cgtgaagttt ctcgacagca
    31741 ccgccgacta cccgcaacag ccgggtgtgg tgctgatcaa agtgccgaaa acactggcat
    31801 tgctggaaca gcaactgcgt gcgctgcgca aagtggtcac gccacaaaca cgtattattg
    31861 ccggtgccaa agcccgtgac atccacactt ccacgctgga actattcgaa aaagtgctcg
    31921 gcccgaccac caccacgctg gcatggaaga aagcgcgcct gattaactgc actttcaatg
    31981 aaccaccgct ggccgatgcg ccgcagaccg ttagctggaa gctggaaggt actgactgga
    32041 ctatccacaa ccatgcgaat gtcttctccc gcaccgggct ggatatcggc gcgcgcttct
    32101 ttatgcaaca tctgccagag aatctcgaag gggagattgt cgatctcggt tgcggtaatg
    32161 gcgttattgg tctgacgctg cttgataaaa acccgcaggc gaaagtggtg tttgtcgatg
    32221 aatcgccgat ggcggttgct tccagccgtt tgaacgttga aaccaacatg ccagaggcgt
    32281 tggatcgctg cgaatttatg atcaacaacg cgctctccgg cgtggagcct ttccgcttta
    32341 atgctgtgct ctgcaacccg ccgtttcatc agcaacatgc gctgaccgat aacgtcgcct
    32401 gggagatgtt ccaccacgcc cgccgctgcc tgaaaatcaa cggcgagctg tatatcgttg
    32461 ccaaccgtca cctggattac ttccataaac tgaagaaaat tttcggcaac tgcaccacca
    32521 tcgccacgaa taataaattt gtggtgctga aagcagtgaa gctggggcgt cgtcggtaag
    32581 attgacagct acctgccaga taaggcgtaa acgccttatc tggcatacgt ttagcacatt
    32641 acccccaggt cggataagat gcggcaagcg tcgcatccgg caatgtctgc ccagttctgg
    32701 aatcgcgctc cataaaacaa aaaatccctg tcaccatcct ctacattctc ttgtttagcg
    32761 ttgtttaacg tttattcttc cgtcacacag ataaattcat ccgttgccaa tctgtcaacg
    32821 gatgttatca tattgccaat ttgtcaacgg acgtgatgaa gaatgcacct gataactcaa
    32881 aaagcattga aagatgctgc ggaaaaatac ccgcaacata aaacggagtt ggtggctcta
    32941 gggaacacga ttgctaaggg atatttcaaa aaacctgagt cattaaaagc agtattccca
    33001 tctctggata acttcaaata tctggataag cattatgttt tcaatgttgg gggcaatgaa
    33061 ttacgtgttg tagcaatggt cttttttgaa tcgcaaaagt gctacatacg tgaagttatg
    33121 acgcataaag aatacgattt ttttaccgct gttcatcgta ctaaggggaa aaaatgattg
    33181 cgattgccga catcttgcat gcaggagaaa agctaactgc tgtggcacct tttctggcgg
    33241 gtattcagaa cgaggaacaa tacatccagg cgctggaact ggtagatcat ctgctgctca
    33301 acgatcctga aaacccctta ctggatctgg tgtgtgccaa aataaccgcg tgggaagaat
    33361 cagcgcccga atttgcggaa tttaatgcca tggctcaagc catgcctggc ggtatagccg
    33421 tgattcgtac ccttatggat caatatggtt taaccctttc cgatctgccg gaaattggca
    33481 gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa attaacgctg gaacacgcta
    33541 aaaaattggc aacgcgattc ggcatttctc ccgccttgtt tattgatta
//
LOCUS       DAZFDC010000040        33522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFDC010000040 DAZFDC010000000
VERSION     DAZFDC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.040"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(133..1302)
                     /locus_tag="ACHJ48_002006"
     CDS             complement(133..1302)
                     /locus_tag="ACHJ48_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4707568.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDIARKQVANGIDPVEERKAQKLAQKLSTKNSFES
                     VSREWHAAKADRWTLAYREEIIKTFEQDVFPFIGKRPIAEIKPLELLEVLKRIEKRGA
                     LEKTRKVRQRCGEVFRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFVRD
                     LEAYTGSIITKNATKIVMLTGVRTQEMRFATWQELDLEKGLWEIPAERMKMRRPHIVP
                     LSRQVIRLFQQLKPITGHYPYIFIGRNNRKKPISKESINQVIELLGFKGRITGHGFRH
                     TMSTILHEAGFNSAWIEMQLAHVDKNSIRGVYNHAQYLNGRSRMLQFYADKLYPN"
     gene            complement(1465..1559)
                     /locus_tag="ACHJ48_002007"
     tRNA            complement(1465..1559)
                     /locus_tag="ACHJ48_002007"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1523..1525),aa:Sec,seq:tca)
     gene            1852..3234
                     /locus_tag="ACHJ48_002008"
     CDS             1852..3234
                     /locus_tag="ACHJ48_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO4707569.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDNFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLCGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3244..5562
                     /gene="yicI"
                     /locus_tag="ACHJ48_002009"
     CDS             3244..5562
                     /gene="yicI"
                     /locus_tag="ACHJ48_002009"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO4707570.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAGRNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTALAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFSEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5615..7324)
                     /locus_tag="ACHJ48_002010"
     CDS             complement(5615..7324)
                     /locus_tag="ACHJ48_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4707571.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSSEVTSTE
                     PVQ"
     gene            complement(7445..8836)
                     /gene="xanP"
                     /locus_tag="ACHJ48_002011"
     CDS             complement(7445..8836)
                     /gene="xanP"
                     /locus_tag="ACHJ48_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO4707572.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9116..10321
                     /gene="gltS"
                     /locus_tag="ACHJ48_002012"
     CDS             9116..10321
                     /gene="gltS"
                     /locus_tag="ACHJ48_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO4707573.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10324..11190
                     /locus_tag="ACHJ48_002013"
     CDS             10324..11190
                     /locus_tag="ACHJ48_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707574.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11175..13256)
                     /gene="recG"
                     /locus_tag="ACHJ48_002014"
     CDS             complement(11175..13256)
                     /gene="recG"
                     /locus_tag="ACHJ48_002014"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO4707575.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13262..13951)
                     /gene="trmH"
                     /locus_tag="ACHJ48_002015"
     CDS             complement(13262..13951)
                     /gene="trmH"
                     /locus_tag="ACHJ48_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO4707576.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13958..16066)
                     /gene="spoT"
                     /locus_tag="ACHJ48_002016"
     CDS             complement(13958..16066)
                     /gene="spoT"
                     /locus_tag="ACHJ48_002016"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO4707577.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16085..16360)
                     /gene="rpoZ"
                     /locus_tag="ACHJ48_002017"
     CDS             complement(16085..16360)
                     /gene="rpoZ"
                     /locus_tag="ACHJ48_002017"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO4707578.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16415..17038)
                     /gene="gmk"
                     /locus_tag="ACHJ48_002018"
     CDS             complement(16415..17038)
                     /gene="gmk"
                     /locus_tag="ACHJ48_002018"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO4707579.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRDQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17296..18978
                     /gene="ligB"
                     /locus_tag="ACHJ48_002019"
     CDS             17296..18978
                     /gene="ligB"
                     /locus_tag="ACHJ48_002019"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO4707580.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAKVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(18975..19592)
                     /locus_tag="ACHJ48_002020"
     CDS             complement(18975..19592)
                     /locus_tag="ACHJ48_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO4707581.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19884..20708)
                     /gene="dinD"
                     /locus_tag="ACHJ48_002021"
     CDS             complement(19884..20708)
                     /gene="dinD"
                     /locus_tag="ACHJ48_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO4707582.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAAVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(20929..21792)
                     /locus_tag="ACHJ48_002022"
     CDS             complement(20929..21792)
                     /locus_tag="ACHJ48_002022"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO4707583.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21919..22635
                     /gene="rph"
                     /locus_tag="ACHJ48_002023"
     CDS             21919..22635
                     /gene="rph"
                     /locus_tag="ACHJ48_002023"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO4707584.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22701..23342
                     /gene="pyrE"
                     /locus_tag="ACHJ48_002024"
     CDS             22701..23342
                     /gene="pyrE"
                     /locus_tag="ACHJ48_002024"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO4707585.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23379..23975)
                     /gene="slmA"
                     /locus_tag="ACHJ48_002025"
     CDS             complement(23379..23975)
                     /gene="slmA"
                     /locus_tag="ACHJ48_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO4707586.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(24082..24540)
                     /gene="dut"
                     /locus_tag="ACHJ48_002026"
     CDS             complement(24082..24540)
                     /gene="dut"
                     /locus_tag="ACHJ48_002026"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO4707587.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24518..25738)
                     /gene="coaBC"
                     /locus_tag="ACHJ48_002027"
     CDS             complement(24518..25738)
                     /gene="coaBC"
                     /locus_tag="ACHJ48_002027"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO4707588.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25910..26578
                     /gene="radC"
                     /locus_tag="ACHJ48_002028"
     CDS             25910..26578
                     /gene="radC"
                     /locus_tag="ACHJ48_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO4707589.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26795..27031
                     /gene="rpmB"
                     /locus_tag="ACHJ48_002029"
     CDS             26795..27031
                     /gene="rpmB"
                     /locus_tag="ACHJ48_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO4707590.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            27052..27219
                     /gene="rpmG"
                     /locus_tag="ACHJ48_002030"
     CDS             27052..27219
                     /gene="rpmG"
                     /locus_tag="ACHJ48_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO4707591.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27317..28126
                     /gene="mutM"
                     /locus_tag="ACHJ48_002031"
     CDS             27317..28126
                     /gene="mutM"
                     /locus_tag="ACHJ48_002031"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO4707592.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28165..28644)
                     /gene="coaD"
                     /locus_tag="ACHJ48_002032"
     CDS             complement(28165..28644)
                     /gene="coaD"
                     /locus_tag="ACHJ48_002032"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO4707593.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28652..29929)
                     /gene="waaA"
                     /locus_tag="ACHJ48_002033"
     CDS             complement(28652..29929)
                     /gene="waaA"
                     /locus_tag="ACHJ48_002033"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO4707594.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30342..31400
                     /gene="rfaQ"
                     /locus_tag="ACHJ48_002034"
     CDS             30342..31400
                     /gene="rfaQ"
                     /locus_tag="ACHJ48_002034"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO4707595.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31397..32521
                     /locus_tag="ACHJ48_002035"
     CDS             31397..32521
                     /locus_tag="ACHJ48_002035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4707596.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32514..33311
                     /gene="rfaP"
                     /locus_tag="ACHJ48_002036"
     CDS             32514..33311
                     /gene="rfaP"
                     /locus_tag="ACHJ48_002036"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO4707597.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVHDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7942 a         8254 c         8884 g         8442 t
ORIGIN      
        1 ttattctctt cgtcactgaa gaattctttt atttttatat atgcctcaat ttgaggagat
       61 ctgagttttt tatttccttc aatatttact ggcgtgtcaa agaaataaga cattttattt
      121 tatccttatt tatcagtttg ggtatagttt atctgcataa aactgaagca tacgagatcg
      181 gccattaaga tattgtgcat gattgtatac accgcgaatg ctgttcttat caacgtgggc
      241 aagttgcatt tcaatccatg cactattaaa accggcttcg tgtagtatgg ttgacatagt
      301 gtgtctgaaa ccatggccag tgattctccc cttaaatccc aataactcaa tcacttggtt
      361 tatgctctcc ttacttatag gcttttttcg gttatttcgt ccaatgaaaa tgtaaggata
      421 gtgaccagtg atcggtttta gttgttgaaa tagccttata acctgccttg aaagcgggac
      481 tatgtgtggc ctacgcatct tcatacgctc tgctggtatc tcccatagcc ctttttctaa
      541 atcaagctct tgccatgtag caaagcgcat ctcttgtgtt cgaacacctg taagcatcac
      601 aatcttggtt gcatttttag tgattatgct gccagtgtat gcctcaagat ctctgacaaa
      661 atgtgggagt tcctctgctg atagaaaagg atgatgtttt tgcttcggca ctgctaatgc
      721 gatagctaaa tcaggtgctg gattgtattc agcgcggcca gtgatgatcg cgtagcgaaa
      781 cacttctccg caccgttggc gtacttttct tgttttctcc aacgctccac gtttttctat
      841 gcgtttaagt acttcaagca gttctagggg tttaatttca gctattggtc gtttaccaat
      901 gaaaggaaaa acatcctgtt caaatgtttt aataatttct tctcgataag ctaaggtcca
      961 acgatctgct tttgctgcat gccattctct gctaacgctc tcaaaagagt ttttagtgga
     1021 taatttctga gcaagtttct gagctttacg ttcttctacc gggtcaatgc cattagcaac
     1081 ctgcttacgg gcgatatcac gcttttcacg tgcttctgcg aggcttacta aatcgtagct
     1141 gccaaatgac attaaacgcg ctttccctgc aaaccggaaa cggaaacgcc agcctttcga
     1201 gccatcagga ttgataagca atgacagacc ttgtccatcg ttcaatgtgt atggtttgtc
     1261 ttgtggtttt gcacgtttga tctgtatatc ggttagagcc atgtgtataa ctgaaaactg
     1321 cgtataaaaa atctatacac attactatac atatttttcg cggattcagg gagatgaact
     1381 cggactatta cggataagct gattattcat cgctttgaat ataaaggatt ttatggagtg
     1441 tctcggaact ggctggatga ggtttggcgg aagatcacag gagtcgaacc tgcccgggac
     1501 cgctggcggc cccaactgga tttgaagtcc agccgcctca ccggagacga cgatcttccg
     1561 cgcctcgatt gctacatgga ggcggggcgc attatagcta cttccttgag tttctacatc
     1621 ccccagatca gttttttccg cctaatacac gcagcgtatt atcacgaaag cattataatt
     1681 catataaaac tcatatttta caatgatata tgagcggaat taacgctacc acgatcacat
     1741 aaaacaagaa tcataaatta ataaccagat atcggaatat tcgctctcac agggatggtt
     1801 tacaaaatgc gttatcggtc tcagcacccc tatagcatca aggaaaagca gatgaagagt
     1861 gaagtgttgt ccgttaaaga gaaaattggt tatggcatgg gagacgccgc cagccacatt
     1921 attttcgata acgtaatgtt atatatgatg ttcttttata ccgataattt tggcatacct
     1981 gccggatttg tcggaaccat gtttttggtc gctcgtgcac tggatgcgat ttccgatcct
     2041 tgcatggggt tgttggccga tcgaacgcgc tctcgctggg gtaaatttcg tccgtgggta
     2101 ctgtgtggcg cactgccatt tgggatcgtc tgtgtactgg cctatagcac gccagatctc
     2161 agtatgaacg gcaaaatgat ctatgcagca attacttaca ccctacttac cttactttat
     2221 accgtcgtca atatccctta ctgcgcattg ggtggtgtaa tcaccaatga cccgactcag
     2281 cgtatctcgc tgcaatcctg gcgttttgtg ctggcgaccg cgggaggcat gctctctact
     2341 gttctgatga tgccactggt taatttaatt ggcggtgata ataaaccact cggtttccag
     2401 ggcggtatcg cggtcctttc cgtggtggca ttcatgatgc tggcattctg tttcttcacc
     2461 actaaagaac gcgttgaagc accacctaca acgacgtcta tgcgggaaga tttacgtgat
     2521 atctggcaaa acgaccagtg gcggattgtc ggtttactaa ccattttcaa tatcctggcg
     2581 gtgtgcgtcc gcggtggagc gatgatgtat tacgtcacat ggattttggg tacgccggaa
     2641 gtgtttgtcg cttttctcac cacttattgc gtgggtaacc tgattggttc cgcactggca
     2701 aaaccgctga ccgactggaa atgtaaagtc actatcttct ggtggacgaa cgccctgctg
     2761 gcagtgatta gcctcgcgat gttctttgtt cctatgcagg ccagcatcac tatgtttgtc
     2821 ttcatcttcg tgattggtgt attgcatcaa ctggtgacac ctatccagtg ggtaatgatg
     2881 tccgataccg tcgactacgg cgagtggtgc aatggtaaac gcctgaccgg gatcagtttt
     2941 gctggcacgc tgtttgtgct caaactgggg ttggccttcg gcggcgctct tatcggctgg
     3001 atgctggctt atggcggata tgatgcggca gaaaaagcgc agaacagcgc cacgattagc
     3061 atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag cgcgattatc
     3121 gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga acagctggcc
     3181 cagggcaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa ctaaggaacg
     3241 gcaatgaaaa ttagcgacgg aaactggttg attcaacctg gcctcaattt gattcacccg
     3301 cttcaggtgt tcgaggttga acagcagggt aatgaaatgg tggtctatgc tgccccccgt
     3361 gatgtgcgtg aacgaacctg gcagcttgat acgcctttat ttacgctgcg ctttttctcc
     3421 ccacaggaag gtattgtcgg tgtacggatt gagcattttc agggggcgct gaataacggt
     3481 cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa cacagaacgt
     3541 tacgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga gttctggtca
     3601 ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa taatggctac
     3661 gtgcaggaca cgaataatca acgaaattat atgtttgagc ggctggatct tggcgttggc
     3721 gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg ccagacggta
     3781 gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa tatcccgttc
     3841 tatatgacta accgtggcta tggggtactg gtcaatcatc ctcaatgcgt ctcttttgaa
     3901 gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata tctcgaatac
     3961 tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg ttttactggt
     4021 cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc atttaccacc
     4081 aactacgacg aagcgacggt aaacagcttt atcgatggta tggcgggacg caatctgcca
     4141 ctgcatgttt ttcactttga ctgtttctgg atgaaagcct tccagtggtg cgattttgag
     4201 tgggacccgc tgactttccc ggacccggaa gggatgatcc gccgcctgaa agcgaaaggg
     4261 ctaaaaatct gcgtctggat taacccctat atcgggcaaa aatcccctgt ctttaaagag
     4321 ttacaagaga aaggctattt actcaaacgc ccggacggtt cgttatggca gtgggataaa
     4381 tggcagccag gtctggcgat ttatgacttt accaatccgg atgcctgcaa atggtacgcc
     4441 gacaaactga aaggtctggt cgcgatgggc gttgattgct ttaagaccga ctttggcgaa
     4501 cgtattccaa tcgatgttca gtggtttgac ggttccgatc cgcagaaaat gcataaccat
     4561 tatgcgtaca tctacaacga actggtgtgg aacgtgctca aggacaccgt tggcgaggaa
     4621 gaagccgtct tgtttgctcg ctcggcctcc gttggtgcgc agaaattccc ggtacactgg
     4681 ggtggtgact gttacgctaa ctacgaatca atggcggaaa gcctgcgcgg tggtttgtct
     4741 attggccttt caggttttgg tttctggagc cacgatatcg gcggctttga gaataccgct
     4801 ctggcgcacg tttacaaacg ctggtgcgcg tttggtttgc tctccagcca tagccgttta
     4861 catggcagca aatcttatcg tgtgccgtgg gcctacgatg atgagtcctg tgatgtggtg
     4921 cgcttcttca cgcaactgaa atgccgcatg atgccgtatc tgtatcgtga agctgctcgt
     4981 gcgaacgcgc ggggtacgcc gatgatgcgg gccatgatga tggagttccc ggacgatccg
     5041 gcttgtgatt accttgaccg tcaatacatg ttaggcgaca acgtgatggt tgctccggtg
     5101 ttcagtgaag cgggcgatgt gcagttctac ttgccggaag gtcgctggac acatctgtgg
     5161 cacaacgatg aactcgatgg tagtcgctgg cataaacagc agcacagctt cctgagtctg
     5221 cccgtttatg tgcgtgataa caccctactg gcgctgggca acaacgagca acgtcccgat
     5281 tacgcgtggc acgaaggcac ggcattccag ctctttaatc tacaagacgg gcatgaagcc
     5341 atctgtgaag tgcccgctgc tgacggttcc gttcttttca ccctgaaagc ggcgcgtact
     5401 ggcaacacga ttactgtgac tggtgcgggc gaggcgaaga actggacact gtgcttgcgc
     5461 aatattgtga aagtaaatgg tctgcaaggt ggttcgcagg ctgaaagtga gcaggggctg
     5521 gtggtgacgc ctcaagggaa tgcgctgaca attacgttgt aatctattga tggaatgccg
     5581 ggtacgtctg agtcgtaccc ggcgagattg atttttactg caccggctct gtcgatgtaa
     5641 cttcgctact ggaaacgacg cccgcattca tggtttgagt cacttgctgc tttgcggcgt
     5701 tcacggcatg gagttgcccg ctaaccgtag gtttcagcgg gacattggcc tgaatatcgc
     5761 cactggcggt aagctgaata ttgccgtcgc cagtcaacgg taattcaggc catccccatt
     5821 gttgcaaaat attcaccggc acaccgcgtc cattcaggct gatatgggtc tgacgttgtg
     5881 gcgtttgtga aacactggca gtggcttcca gaatgccttt ttcagtaaat gcactcagtt
     5941 cgctgatatt caccgtgctg ctgttggcgg tcagcgccag cgacgggcga cgaacatcaa
     6001 cacgattgaa tgtcgcggcg gcggcattca gattcgccga gccactccag acgccccatt
     6061 tatgatcggt aaccagcgtc aggttggcac cgtaaccatc gagcgtggtg agctgccacg
     6121 ggaagtcagg gtcgatatca atgatcagat tgcggctggc gctaaatctc ttcagttgca
     6181 ggctgtttaa ccaaccgggt gtcgtttcca tccacaactg ttgccagttt ttcggcaagg
     6241 tatattccag cccggcaatt gccgcatcat caaggatcaa cgttttcccg tcacgcagcc
     6301 agttccctga cgttctgacc ataccccctt cccagcggct ggtgaactgg cgcagcgcta
     6361 cgccctgcgg ggaaaattcc gcgtttataa tcgggtcaaa taaatgcagc gaaccataaa
     6421 tgaactcgct agcgttcatc gacagtttgc cttcttgtgt ctgccagtca tctttactga
     6481 aggtcatgtt gcgcaagctg agatcgaggt cggtcaccgc ccagtccgga ccttgcaaac
     6541 gagcatcgat cacttccagg cgaccaatct gcaacgacgg gacagagcgt aatggcgcaa
     6601 agaagtcggt tagcgatttt tcgctttgta gacggatatc cgccatgcgc agattttcca
     6661 cttgccagct gccgtcggcg ttacgctgcg cgtttccggt taatgtcccg cgggcgatgt
     6721 cggcacccag gttagtcagc gtaacgcgat cgttatcaat actgccttca atcagtacat
     6781 tggtggcagg aacatcattg agcgaaagcg atccggcact aaactgaatc tgcgccttcg
     6841 tacccagcac tttaccggct tctggtgacc acggaaccac gccgccattt acccgctgcg
     6901 cgctcagttt ccattcgcta ttcgggctat taaacgccat atcacgcagt tgcagacgat
     6961 cggctttgaa cggtagcggc gcggtctggt cggtgagatt cagcgtcccg ttttccagca
     7021 ggatggtatc gacatggcgt ggttcggtca gttgccgact gcttagcgca atgtcgacac
     7081 tttttgccac cagggtcgcg ggctgaccat cacgaccaaa cgtgacgttc tccagcacga
     7141 tatgagatgg cgcggaaaaa cggtgatcca tcgccccgaa ggccagatga tagtcgctat
     7201 tctcggaaac ccatgcgctg atatgttctg ctccccagcg ggtttgcaga agaaaataga
     7261 ggccagcgat caccactaac agagcgatga gaatgtagag aagcagcttc ccaataaatt
     7321 tcatggtctt ccatcccgtg aaatgcacat aagggagtta tgcacgattt acgcgcaatc
     7381 ctcaaggcgg gaatggtgaa agaggtgaga ctgcggcggt aattatctta ccgccgccag
     7441 tgaattactg tttttctggt gggaaaatca gattcagcac gatggcagta ataccgcccg
     7501 cggcgatccc ggaggagagc aggtttttca gccattcagg ggcaaactgc aaaatcagcg
     7561 gctgctgaga cacgcccaga ccaaccgcca gcgacagcgc gataatcaga atcgcccgac
     7621 ggttcagcgg ctcacgagaa acgatacgca caccggaggc ggcgatggtg ccaaacatta
     7681 caagcgttgc gccgcccaga accggttctg gaatgtgttg tacaaaaccg ctcactgccg
     7741 ggaacagacc cagcacgatc aacatcagcg cgacgacaaa accgacatag cggctggcaa
     7801 caccagtcaa ctggatcact ccgttgtttt gcccgaagca ggagttcggg aaggtgttaa
     7861 acaccgccga aacaaacgag ttcaggccgt ttgccagcac gccgcctttc aggcgtttca
     7921 tgtacagcgg accggacact ggctgttcgg aaacgtcaga ggtcgccgtg atatcgccaa
     7981 tggtttccag cgaagtgatc ataaagacca gcatcagcgg cagcagcaga ctccattcaa
     8041 tgccaagacc gtaatagagc ggcgttggca ccataatcag ttcttgcgtc atcggttcgt
     8101 tgctttctgg caacatgccc ataaaccacg ccagcgcata tccggccgcc atcgcaatca
     8161 ccagtgaggc cacgcgtaag taagggttac gttgacggtt aagcaggata attaaggcta
     8221 agaccacgcc tgccagcagc agatttttcg gtgcgccgaa ggtgttatcg ctcatggctg
     8281 cgtaaccgcc gccaatggac gttaacccaa cctgaattag cgacaggccg ataatcatca
     8341 ccacaacgcc agaaaccagc ggcgtaataa tgcggcgcgc cagatgcaga acgcgggaga
     8401 tcaccatctc ggtgcaactt gccagcatca acgtgccgaa caaagccgcc atcatggtag
     8461 gaacatcagc accaccggtt ttcagcgcgg taccgcccat aatcagcggg gcaacaaagt
     8521 tgaagctggt gccctgaata gacaacagcc cggagccaac cggaccccag gccttaattt
     8581 gaataatcga tgccacaccg gaggcaaaca gcgacatact aataatgtgt tgcgtgtctt
     8641 gtgccggtaa acccagcgcc tggcagatta atagcgctgg cgtgatcacc gcaacgaaca
     8701 tcgccagcag atgctgacag gcggcaaaca gggtttgagg aagcggcgga cgatcttcaa
     8761 gacggtaaat cagttcgctg ttttgagtct gcgcaaccgg ttgcgcattt tctgactcga
     8821 gggtggaaac agacatcgaa aacaatcccg tggtagcaaa gcgggcattt tagctgaccg
     8881 aaagagaaaa gcaaacgttt gccacatcct catctaaata tttaaatgag ataaatgcac
     8941 ttttttatat aacttttggt atttttctgc ttaaaatcca tgccatttgt tcattaacag
     9001 ctgagaattt gacccaggaa agcataactc gatacccgtc atagttcatg aagttgccac
     9061 tgcgcgatga agtatgacga gtatgaaaga gtgatgcgga tacaaaggag taactatgtt
     9121 tcatctcgat actttagcaa cgcttgttgc cgcaacgctg acgttgttgc tcgggcgtaa
     9181 gttggtccat tccgtctcct ttttgaagaa atacaccata ccggaacctg ttgcgggtgg
     9241 tttgttggtg gcgctggcgc tactagtact gaaaaaaagc atgggctggg aagtcaactt
     9301 tgatatgtcc ctgcgcgatc cgttaatgct ggctttcttc gccaccattg gcctgaacgc
     9361 caacattgcc agtttgcgtg ccggtgggcg tgtggttggc atcttcttga ttgtggttgt
     9421 tggtctgttg gtgatgcaaa atgccattgg cattggtatg gctagcctgt tagggcttga
     9481 tccgctgatg gggctgttgg ccggttctat tacgctttcc ggcggtcacg gtacgggcgc
     9541 tgcgtggagt aaattgttca ttgaacgtta tggcttcacc aatgcgacgg aagtggcgat
     9601 ggcctgtgca acgttcggtc tggtgctggg cggcttgatt ggcggtccgg tggcgcgcta
     9661 tctggtgaaa cactccacca cgccgaacgg tattccggat gaccaggaag tcccgacggc
     9721 gtttgaaaag ccggatgtgg gccgcatgat cacctcgctg gtgctgattg aaactatcgc
     9781 gctgattgct atctgcctga cggtggggaa aattgttgcg caacttttgg ctggcactgc
     9841 ttttgaactg ccgaccttcg tctgtgtact gtttgttggc gtgattctga gcaacggtct
     9901 gtcaatgatg ggcttttacc gcgtctttga gcgtgcggta tccgtgctgg gtaacgtaag
     9961 cctgtcgttg ttcctggcga tggcgctgat ggggctgaaa ctgtgggagc tggcttcgct
    10021 ggcgctgccg atgctggcga ttctggtggt acagaccatc ttcatggcgt tgtatgccat
    10081 cttcgttacc tggcgcatga tgggcaaaaa ctacgatgcg gcagtgctgg ctgcgggtca
    10141 ctgtggtttt ggcctcggtg caacgccaac ggcaatcgcc aacatgcagg cgatcactga
    10201 acgctttggc ccgtcgcaca tggcgttttt ggtggtgccg atggtcggtg cgttctttat
    10261 cgatatcgtc aatgcgctgg tgattaagct gtatttaatg ttgccgattt ttgccggtta
    10321 aaaatgaaaa agcgtcggaa ggaaccggaa acgctacgtg aacactgtcg ccatattttt
    10381 ggtgatgagc cgccagtgct ctgtgtatgg gaaacggaat ttgattacgc agatgccgaa
    10441 ctcaaggcgc tggcggctaa agagtggcag cagatcagcg aatgggattt gagtgcttac
    10501 tatgtactca atctcgttta taacgaaccg atgcaaatag agttgtttcg ctatctgttt
    10561 ccgctctgcc tggcgcaatg gcatgaaacc gttttagctg gcggttatgg tgatcacttc
    10621 gaagaatcct taatgaaggc attgtgtcgg ccctatctct ggcaggagat gatgaatgct
    10681 tcccaacgcc agcaggtacg gcaattttta ctggacaccg cgttgcagcg tatggataac
    10741 gagcgtggtt tcaataacgt tctttgctgg ctggcggtgt tcaatactct gggtggggcc
    10801 gcgccgttaa ttcgttctct ctggtcgcgc tggtgggcgc tggatacgcc aggaaaagcg
    10861 gtgtgtgcta ttcagtatgc cgcgcatcta atttatccca tagaagccaa tccgttatgg
    10921 tcgcaagaat ggataggctg ggggcatcca ttaggccata aagatggctg gtcctctgat
    10981 aatcgcgctt ttcttcgcca aatgttaaca cctgaaatga ttgtggctgg cgtacaggca
    11041 gcggctgaaa ttctgcgtgg tgaaccggaa ggcgcaatgg cggcgcgaat tgcacaggat
    11101 gcgtatgagg caatggatat cctcactatc cagattgaag atttactgcg tgacttgtct
    11161 tgtgatgagt ctggtcatgc gttggagtaa cgttccgtct ccggcatcca gcgttctatc
    11221 agggcttttg cttgttgtgg gtaacgttcg tgaatatgtc gcgccaggcg ctgaacttcc
    11281 gggatcatcg cctgatcgcg cagtaaatcc gccactttaa actcagcatt acccgtctga
    11341 cgcgtaccta acagttcacc cggtccgcga atctctaagt ctttctgtgc aatcacaaaa
    11401 ccgtcgttgc tgtcgcgcag cacttgcagc cgaatttgcg cggttttgga gagcggcgtt
    11461 ttgtagagca gcacgcagtg agaagccacc gcaccacgac ctacacgccc gcgaagctgg
    11521 tgtaactgcg ccagacccag acgctcaggg ttttcgataa tcatcaggct ggcgttaggc
    11581 acatcaacac cgacttcaat aacggtagtg gcaactagca gatgtagctc accttgttta
    11641 aacgacgcca tcaccgcctg tttctcggct ggtttcatcc gcccgtgtac caggccaaca
    11701 ttcaattctg gtagcgccag tttcaactct tcccaggtag cttccgccgc ctgcgcttcc
    11761 agtaactctg actcttcaat caacgtacaa acccagtacg cctgacgacc ttcagttatg
    11821 caggcgtggc gcacgcggtc aatgatgtcg gtacggcggg tatcaggaat agcgaccgta
    11881 gtcactggcg tgcggcctgg cggcagctca tctatcaccg aggtatcgag atcggcatac
    11941 gccgtcattg ccagcgtgcg ggggatcggc gtggcggtca tgatcaactg atgcggatgg
    12001 aagccttgct gctggccttt ctcccataac gccagacgct gatgcacgcc aaagcgatgt
    12061 tgttcgtcga taatcaccag cgccagaccg ttaaactgca cctgttcctg gaagatggcg
    12121 tgcgtaccaa caatcatctg cacctgaccg ctggcgatgg cttcctgctg agacagtcgc
    12181 gctttgcctt tctgcttacc ggcgagccag cccacttcga taccgagcgg ttcaaaccag
    12241 ttgcggaagt tattggcgtg ctgttcggcg agtaattcgg ttggtgccat caatgccacc
    12301 tgtttgccgt gggcaatcgc acgcagcgcg gcgagggcgg cgaccagcgt tttaccggaa
    12361 cctacatcgc cctgtaccag gcgcatcatc ggcacatcca gcgccatatc gcgctcgatc
    12421 tccgccacta cgcgtgcctg tgcgcccgtt ggcttgaacg gtaaggctgc gaggagttta
    12481 tttttcagcg catcattggc gctcagcggc tgggcatgaa aacgctgtgc tccggcacgt
    12541 aaggctaaca tgctgaggtt gtgcgccagc agttcttcca gaatcagacg acgttgcgct
    12601 ggatgctgcc cggtttccag atcgctaagc tgtagcgtcg gtggcgggcg gtgcaaagtg
    12661 cgcaacgctt ccggtagcgt catcattcct tgtgacagtt ccggcggcag gagttcttca
    12721 atggcgcagg tgtcgagcag atccagcgcc tggtcggtta atttacgcag cgtggcctgc
    12781 tttacgcctt ccgttgttgg ataaaccggc gtgagcgttt cctgtaattc tggcgtgctg
    12841 agatcgccct gcacgcggta ttccgggtgg atcatctccg caccatattt accgcgcttt
    12901 gcttcgccat aagccagtac acggcggccc gtcgccaggc tatttttcat tgccgcgctg
    12961 aaattgaaaa agcgcatggt gaggatgccg gaaccgtcgc tgatctggca ggtcatcatc
    13021 cgccgaccgc cgaaggagat attgcagttc agcacttcgc cttccaccgt ggcataaacg
    13081 cccggcagta gttctccgat ggggtagaga tgggtgcgat cttcgtagcg caggggaagg
    13141 tgtaagagta gatcctgcac ggtatgcagg ttgattttcg ccagtttgtt actaagtgct
    13201 gcgccaacgc ccgttaggga actgagtggg acagcatcta acaggcgacc tgtcatggca
    13261 cttaccctgc agcctgcata gtagcccacc agtcggcatc agcttcgatc tcgccttgct
    13321 gattgacgtg gggataaggc aggcctttgc gttttgcgac tttcgccagc accggatagc
    13381 cgccttcaaa caacaggcgt tgttgctctg cttccggcaa catgctgttt tcacgcaggt
    13441 acatgcctgc attttgccgc tgacgctggg cttcgtaaag aatgagggct gaggcaacgg
    13501 aaacattcag cgactgcacc atgccgatca tcggaatgat gatgtcctga tccgccaggg
    13561 ccaatgcttc ctgcgtgatg cccgttttct cctgtcccat caaaatgcag gtcgggcgag
    13621 tgtaatcaat ttcgcggaaa tcgacagcgt tatcagaaag atgggttgcc agaatctgca
    13681 tgccctggcc tttgagatga gcgacggcat cgccaatggt gcggtgtgtt ttcacctgta
    13741 cccagctgtt actacccgcc gctgccgaag ccatggtgcg catgcggcta ccaggccaga
    13801 cggcgtgaac ttcatgtacg ccaacggcat ctgcggtacg aataatcgca gaaacgttat
    13861 gaggtttgtg gacctgctcc atgcagacgg tcagatcagg ctgccgcctg gcgagcattt
    13921 cgcagatgcg tgcataacgt gttgggttca taaaacatta atttcggttt cgggtgactt
    13981 taatcacgtc tggcatcacg cggattttgc gcatgatatt cgccagatgc acacggtcac
    14041 gagcggtcag acgaataaag gcgctgtaga cgcgaccatc tttctcttcc gtattcaaac
    14101 tttgaatatt cgaggtcgtg gtgttaattg ccgccgtcag gtttgccagc gcaccctgat
    14161 gattgaacat ctccaccttg atttcggtga tgaactcctg cgccgtctct ttatcccatt
    14221 ccacagccat aaacttctct ggctctttct ggtagccacg gatgttacgg caggattcat
    14281 ggtggatcac cagaccttta ccggggctga cgtgggcgat aatcgggtcg ccaggaatgg
    14341 ggcggcagca tttcgcaaag gtgatcagca cgccatcagc gcctttgatc ggcagatgcc
    14401 cgtggctttg agtcgccggt ggaatggagg cgtccccatg ttgtagattt ttcgcgacca
    14461 ccacgctcat tgcgttacca aggccgattt ctgccagcag atcgtcaagc gttgccagct
    14521 tcatgcgatc cagctcgcgc tgaatatttt cctgcgggat ttcattgagc ttacggctac
    14581 cacccaaagc atggttgagc agacgacggc ccaggcttac agaatcatca cgcttgaggt
    14641 ttttcagcaa ctgacgaatt ttggcgcgcg ctttcgagct aacgacaaag ttcagccaag
    14701 cggcattcgg gcgagcgccc ggagcggtaa tgatttcaac ggtctgaccg ctagtaagcg
    14761 gctgcgacag cgggtaaggc tggcggtcga cgcgtgcgcc cacgcaggca tggccgatat
    14821 cggtatgcac tgcataagcg aagtcgacgg gcgttgcacc ggcaggcagc tcgacaatgc
    14881 gcccttccgg tgtgaaaacg taaatctcat ccgggaagag atcggattta acgctctcga
    14941 taaattcaaa cgaactaccg gcgctctgtt gcagctccag caggctttgc atccagcgct
    15001 gggcgcggat ttgtgcggta gtactggttt cgccgtgctc tttataagcc cagtgcgcgg
    15061 caacacccat ctccgccatc tgatccatat cttcggtacg gatctggacc tcaaccggca
    15121 cgccgtgcgg gccaatcatc gaggtgtgca acgactgata gccgttcgct tttggaatgg
    15181 cgatatagtc tttcacgcgg cccggacgcg gcttgtacag gctgtgcatc tggcccagca
    15241 cgcgataaca ggtgtcagaa tcattgacga tcacgcggaa agcgtagatg tccatgattg
    15301 agtgaaaacg ctgctctttg agcaccattt tacagtaaat cgaataaaga tgcttttcgc
    15361 gaccactgac gcggcacggt attcccgctt cctgcaaacg cccttcgatt tcagaaagaa
    15421 tcttctggat catctcttta cggttgccgc gcgcggcttt caccacttct ttgattacgc
    15481 gataacggtt gggatacagc gcctcaaaac ccagctcttc gagttcggtt ttaatgtggt
    15541 ggatacctaa acggtgcgcc agcgggctgt aaatttcgag agtttcacgg gcgatgcggc
    15601 gacgtttgtc cgggcgaagt gagcccagcg tgcgcatgtt gtgggtacgg tcggcaagtt
    15661 tgatgaggat gacgcggata tcctgcacca tcgccataat catcttgcga aagttttcgg
    15721 cctgcgcctc tttcttatcg cggaacttga gtttatcaag tttcgacacc ccctctacca
    15781 gctcggcgac gcttttacca aaaagctgtt ccatatcctg gtaggtggcg ggagtatctt
    15841 caatcacgtc atgcagcagc gccgccatca gcgtttcata gtcgagtttc atctcggcca
    15901 gaatgcaggc aaccgctacc gggtgcgtga tatagggttc accgcttgaa cgtgtttgcc
    15961 cctcgtgagc atcacgtgca acgagatacg cctgccgcag acgcttgatt tggtcttccg
    16021 gcaggtaggt ttgaatcagt tgattcaggc tttcaaacag atacaagggc gacccgcttt
    16081 gtgattaacg acgaccttca gcaatagcgg taacggcttg taattcagcg gcttcctgct
    16141 cttgctgttc ctggcgttcg cgaacgtcga ggatctggtt gttgatcaga ccttcttcga
    16201 tttcgcgcag cgcgattaca gtggttttat cgttttcttc cggtaccagc ggatcctttc
    16261 cgcctacctg catctgacga gcgcgacgcg cggcgaccag taccaggtca aaacggttac
    16321 caattttctc tacagcgtcc tgaacagtta cgcgtgccat acttaaaaag ctccacaggt
    16381 gaagaaatga ctgggcatga tactgaaatc aggttcagtc tgccaacaat ttgctgatta
    16441 aagcgtcatg acgctgcttt tggcggctca tgcgcagacg ttcggcgcga ataatggtct
    16501 tcaaatcggt caacgcggta tcgaagtcat cattcacaat caggtaatca tattcggcgt
    16561 aatggctcat ttctgcaaca gcttgcgcca tacgctttgc aatgacctct tcgctgtcct
    16621 gatcgcgacc gcgtagacgg cggtccagtt caattttaga cggcggtaaa ataaagatac
    16681 tccgcgcgtg cggcatcttc tggcgaattt gctgcgcgcc ctgccagtcg atatcgagaa
    16741 aaacatcgac accggtcgcc agtacttgct caatggcctc acgcgaagtg ccatagtaat
    16801 taccaaaaac ttctgcgtgt tcgaggaacg catctctgct aatcatttct ttaaattcat
    16861 catgattaac aaagaaataa tgttcaccgt ggacttcacc aggacgcggt tggcgtgtgg
    16921 tgtgtgaaac agaaacctgg gtgtcataca acggttgggt ttttaataaa gcctgaatca
    16981 ggctggattt acccgcgcca ctgggggcag aaacaatata aagcgtgcct tgagccatga
    17041 gtatctttcg tatgtgatta gcgaaataaa gcctacatac gagcttatta tacacggcgc
    17101 ggccatgtga cgtagccctt gctcacactt tttgcgcaaa gttgttgcgt tttgcgtagc
    17161 tgtttccgca tttgcctgtg atttactgca ataaagacga ttctcaggaa gcgtcctcgc
    17221 agactccgtt tttgccactc ggctttgcct tcaccacgtt gtatatctgc cagcctgact
    17281 acagcggagg tgaagatgaa agtatggatg gcgatattaa taagtatctt gtgctggcaa
    17341 tcatctgtgt gggcggtctg tccggcctgg tcgccagcca gagcacagga agaaattttc
    17401 cgcctgcaac agcaaataaa acagtgggac gatgactact ggaaggaagg aaaaagtgag
    17461 gtggaagacg gtgtttacga tcagttaagc gcccgtctta cgcagtggca acgctgcttt
    17521 gtgagcgagc cccgcgacgt gatgatgccg cctttaaatg gtgcggttat gcatccggtt
    17581 gcccatacgg gtgtacgcaa aatggcggat aaaaacgcat taagtctgtg gatgcgagaa
    17641 cgtagcgatc tttgggtgca gccaaaagtt gatggcgttg cggtaaccct ggtttatcgg
    17701 gacgggaaac tgaacaaagc aatcagtcgc ggtaatggcc tgaaaggcga ggactggacg
    17761 cagaaagtta gcttaatttc cgctgtgccg caaaccgtta gcgggccttt agccaacagt
    17821 acgcttcagg gggaaatatt tctccagcgc gaggggcata tccaacaaca aatgggggga
    17881 ataaatgccc gcgcaaaagt tgctggcctg atgatgcgcc aggacgatag cgacacgctg
    17941 aattctctgg gcgtttttgt ctgggcatgg ccggatggac cgcagttaat gaccgatcgt
    18001 ttaaaggagc tggctaccgc aggttttact ctaacgcaga ggtatacccg tgcggttaaa
    18061 aatgctgatg aggttgcgcg cgtacgcaat gagtggtgga aagcgaaatt acccttcgtc
    18121 accgatggcg tggttgtgcg aggggcgaaa gaacccgaat cccgccattg gctaccaggc
    18181 caggcagaat ggctggtggc ctggaaatat caacctgtag ctcaggttgc caaagtgaag
    18241 gcgattcagt ttgcggtggg taagagcggt aaaatatcgg tggttgcgtc actcgcacct
    18301 gtcatgctgg atgataaaaa agtccagcgg gtgaatattg gttccgtcag gcgctggcag
    18361 gagtgggata ttgcgcctgg tgatcagatc ctcgtcagcc ttgccggtca gggtattcct
    18421 cgcattgatg atgtggtctg gcgcggtgca gaacgtacaa aaccgacacc accagaaaac
    18481 cgctttaact cgttgacctg ctactttgct tctgatgttt gtcaggaaca gttcatttca
    18541 cgcttagtct ggctggggtc aaaacaggtt ctggggctgg atggcattgg tgaggccggc
    18601 tggcgcgcgc tgcatcagac tcatcgcttt gagcatatct tttcctggct tttattaacg
    18661 ccagagcaat tacagaacac gccggggatc gcgaaaagta aaagtgcgca gctatggcat
    18721 cggtttaatc tggctcgtaa gcagcctttt actcgctggg tgatggcaat gggaataccg
    18781 ctaacccggg cggcgcttaa tgccagtgat gaacggtcct ggtcgcaact tttatttagc
    18841 acggagcagt tctggcagca gctgccgggg actggatcgg ggcgcgccag acaggttatt
    18901 gaatggaagg aaaatgcgca aatcaagaag ctgggcagtt ggctggctgc ccagcagatc
    18961 acaggttttg aaccttagtg tccttcgtgg ctgtagtaaa agaccggtaa tcccagctta
    19021 aggcgtagcg ccagtaagcg ggcaaaaaag ccgaatacca gggtggagat aattaccaca
    19081 tcatggttag aaacatagtg ttgcagcgcg atgtacagca cggcggaggc aaatgagaca
    19141 ccggcataca actctttctg gaataccagt gggatacgtt tacagaacat atcgcgcaaa
    19201 acgccgccaa acacgccggt ggttaccgcc gcgacaacgg caataattgg gccgtgcccc
    19261 atatccagtg caacctgtgc gccgatgata gaaaagacca ccagtccaag cgcatcgagc
    19321 accagaaaca ctttgcgcag gtaaggcatt acaggagcaa cgatagtggt gagtactgcg
    19381 gcggtagcga cgataatcac atattccggg tgtttgaccc agccgagcgg atagtggccc
    19441 agcagaatat cgcgcactga ccctccgcca attgcggtgg cggtcgcaat aataattacg
    19501 ccaaatgtat ccatgcggcg tcgcccggcc gccagagccc ccgtcatggc ttcggcagta
    19561 atgccaacca aatacaaaat gtgtaacagc atggttctct ccctaaaaat ctgtctggca
    19621 gggtagcgat ttgtgcgtat tgtcacgatt gagatttttt aaggtgagag gaaattgata
    19681 acttgtagtt atgtgaaatc gctattttct gtagcgtaga tgcattcttc tgacttcttg
    19741 tctgagaaaa tgcataagaa aaaataggtt aaaattgagg ggtggaaaag aaaggtgcaa
    19801 tgtttagcca ttgtgttcac cgcacctgga ctttggctta cgcatcgggt atctcaatca
    19861 acaaaaagag tttttagttt ctgctatttc ttctctgtaa tttttacact gttttccaac
    19921 tgcttgatgc tgacctgcgg ggtcggcaac tcctcaggca tggttccgcc aagttcctga
    19981 atggtttgcc tcaccttgct acccacatca aagtgtgtgg tattagcttg ctgttttgaa
    20041 ttcacgccat cccgcttgag tttttcttct gtttgggtag ctcgaaagag attagccgcc
    20101 agttctgttg aacccatatg atccaggatc ttctgactct ttttcagccc cttcagttgg
    20161 tggatggctt tctgatctaa tccaccatac agcccctggt aaccatgatt ctgaaatatg
    20221 gcgaaatcag tagctgtcgc taccgcagct tgctgtgctg cctcaaccaa ttgtttgtta
    20281 tgttctttca actcatttct cagaaacaga cgcttttcgt cttcacgaag ttgcttaaat
    20341 gcttcatcgt cagcaagctc ctgccgtcgg gtctggatag caaaataagt ctgccctgcc
    20401 gcaatgaccg gtttcgcagg gtcgccgttt tgtactacca ggtaacaggc atagcgggag
    20461 agatgaacat cttcaagttc tcgttgagcc cctgaaccta atacaaccat tttggtggtt
    20521 tcaacgaaat ggtcagatgc agcttgattg ctggcttcgc acgcttgagt cgcgcgggcc
    20581 agtacctttt gaaaattgcg ccagtcccga taatccagta acggtgccaa ttctctagct
    20641 gaccaaaatt cttgcccttc agtgccataa tgccgtattt cttcaaaagg ttgatgatgt
    20701 tcgttcattg cccactccat gtgatatcga ttgcaaagaa agtaaatctg taactgtatt
    20761 tatatacagt tgtgagtatc tcacgttctc agtgtgctgg gcaaaaaaac atcacaaaat
    20821 ctggagcagg cacgcaaaac agaattgaag ttgcggggaa gaaaaatgcc gctctgtttg
    20881 agtgggcggc atttgtgaag gctgaaaagt aagaattggc gttacgagtt attcaatgtt
    20941 ctgaatctgc tcgcgcatct gctcaatcaa cactttcagc tcaatcgcgg agtttgtcac
    21001 ttcggcattg atagacttcg acgcaagagt gttcgactcg cggttgaatt cctgcatcat
    21061 aaagtccaga cgacgaccaa cggcttcttt tttcttcaga atgttgtagg tctctttgac
    21121 atgcgcttcc aggcggtcca gttcttcggc aacgtcaatt cgttgtgcca gcagaaccag
    21181 ctcctgctcc agacggttgt tttccaactg aacctgagca tcttccagct tcgcgaccag
    21241 acgctcacgc tgccattgca ggatttccgg catatgggcg cggactttga ccacttcggc
    21301 ggtgacgcct tccagacgct gctcgatcaa tgctttcagt gcctgacctt cggtttcgcg
    21361 cgcgacaata aagtcgtcca gcgtaccatc cagcgccgcg agaatttcag cggcaatggc
    21421 gtcaagatcc tgctcctggg ctgccatcac gcccggccag cgtagaatat caaccgggtt
    21481 gatttcccct tcgtcactct gcatttttac ccagttcgcg gcagttacca gctgtttagc
    21541 cagtttttcg ttgaggatca gctcaccttg cgcgctaaca tctggctcat agcgcagggt
    21601 acattccact ttaccgcgcg tcaggcgaga acgaatacgc tcgcgaacga caggttcaag
    21661 gctacggaac tgctccggca gacgaaagta agtttccaga taacgctggt ttaccgagcg
    21721 catttcccag gttgcgctcc cccattcacc cttgatttca cgccgggcgt aggcggtcat
    21781 actgcggatc atagacgttc ctgtttataa aaggagaggt ggaaggatta tagccatcga
    21841 tgccttgtca ggataggaat aaccgccgga agtccgtata atgcgcagcc acatttgttt
    21901 caagccggag atttcaatat gcgtccagca ggccgtagca ataatcaggt gcgtcccgtt
    21961 accctgactc gtaactatac aaaacatgca gaaggctcgg tgctggtcga atttggcgat
    22021 accaaagtgt tgtgtaccgc ctctattgaa gaaggcgtgc cgcgcttcct gaaaggtcag
    22081 ggccagggct ggatcaccgc agagtacggc atgctgccac gttctaccca cacccgtaac
    22141 gctcgtgaag cggcgaaagg taagcagggt ggacgcacaa tggaaatcca gcgtctgatc
    22201 gcccgtgctc ttcgcgcggc agtagatttg aaagcgctgg gtgagttcac cattacgctg
    22261 gactgcgacg tgcttcaggc tgatggtggc acgcgtaccg cgtcgattac gggtgcctgc
    22321 gtggcgctgg cagatgcgct acagaagctg gtggaaaacg gcaagctgaa aaccaacccg
    22381 atgaaaggga tggtagccgc agtttctgtc ggaattgtga acggcgaagc ggtttgcgat
    22441 ctggaatacg ttgaagactc tgccgcagag accgacatga acgtagtgat gaccgaagac
    22501 gggcgcatca ttgaagtgca ggggacggca gaaggcgagc cgttcaccca tgaagagcta
    22561 ctcaccttgt tggctctggc ccgaggggga atcgaatcca ttgtagcgac gcagaaggcg
    22621 gcgctggcaa actgattttt aaggcgactg atgagtcgcc ttttttttgt ctgtagaaaa
    22681 gtaagatgag gagcgaaggc atgaaaccat atcagcgcca gtttattgaa tttgcgcttg
    22741 gcaagcaggt gttaaagttt ggcgagttta cgttgaagtc cgggcgcaaa agcccctatt
    22801 tcttcaacgc cgggctgttt aataccgggc gcgatctggc actgttaggc cgtttttacg
    22861 ctgaagcgtt ggtggattcc ggccttgagt tcgatctgct gtttggccct gcttacaaag
    22921 ggatcccgat tgccaccact accgccgtgg cgctggcgga gcatcatgac cttgacctgc
    22981 cgtactgctt taaccgcaaa gaggcaaaag accacggtga aggcggcaat ctggttggta
    23041 gcgcgttaca aggacgcgta atgctggtag atgatgtgat caccgccgga acggcgattc
    23101 gcgaatcgat ggagattatt caggctaatg gcgcgacgct tgctggcgtg ttgatttcgc
    23161 ttgatcgtca ggaacggggg cgcggcgaga tttcggcgat tcaggaagtt gagcgtgatt
    23221 acaactgcaa agtgatctct atcatcaccc tgaaagacct gattgcttac ctggaagaga
    23281 agccggaaat ggcggaacat ctggcggcgg ttaaggccta tcgcgaagag tttggcgttt
    23341 aaagaaactc gccggatgaa aagtcatccg gcgtcatatt actgcaactg tgccgcaatt
    23401 agcggccagc gggcgtcaaa atcatccgtc gggcggtatt taaattcgct gcggacaaaa
    23461 cgtgacagca taccttcaca gaaggctagg atctggcttg ccagcagggt ttcatcggtg
    23521 gtgtaacctt caccctcacg cattctcttt tcacgcaata cctgacgcag ctgcgcttca
    23581 atacgctcga acagctggtt gatgcgccct tgcaggcgat cctgttcaaa cattagcgca
    23641 tgaccagtga ggatgcgggt caggccagga ttacgctcac caaaaccgag aagcagcaac
    23701 acaatcagac gcaggcgcgc tgtggtgtct ttctcatctt tcagaatcag attgatgcga
    23761 gtaatcaggc tatcttcgat aaactcaatc aggctatcga acatgcgggt cttactgggg
    23821 aagtggcgat acagtgccgc ttcggaaacg ccgacagagg cggccagttt tgccgtcgtg
    23881 atacgttggc ttccatcgct ggattccagc atcagcgcca gagactgaag tatttcctcg
    23941 cgacggttcc ttttcgcagt ttgtttttct gccatgttac aaaatacccc tgaaaataag
    24001 cacttgccag gcggcaccca cgctatgacc gcaaacgaaa tgtttacggc tatgttatga
    24061 cgttattcgg atgcgtatgt gttactgacg accagagtga ccaaagccgc cttcaccgcg
    24121 gtcggtggcg tcgaaatctt ccaccagatt aaattcagcc tgtactaccg gaacaaaaat
    24181 catctgggcg atacgttcgc caggttgaat ggtgaagctg tcctgaccac ggttccacac
    24241 ggaaatcatc aactggccct gatagtcgga atcgatcaat cctaccaggt taccaagcac
    24301 gataccgtgc ttatgtccca atccggagcg cggcagcatc attgccgcca gtgaaggatc
    24361 ggcaatatga atcgccagcc cggtcggaac cagcgtagtg tcacccggag ccagttctac
    24421 ggcgtcgtcg agacaggcac gcaggtcaag tccggcagag ccagaggtgg cataagtcgg
    24481 gagcggaaat tccttcccaa cgcgcgggtc cagaatctta acgtcgattt ttttcatcat
    24541 aacgggtcac gatctcgtcg agtaataatt ggccaaggag ctctttgcgc tcaagcggta
    24601 agactttatc tccgtcctgc cagaaaaggt gtaatgcgtt gttgtcgctg ttaaatcctt
    24661 gagttggctg ggaaacatcg ttcgcgcaga tcagatcaag gtttttacgg atacgttttt
    24721 gccgggcgta ttcttccaca ttatttgttt cggcggcaaa tccaacgacg tagggtcgat
    24781 ggtcttttag tgcggcaacg cctgcgacga tatcggggtt tttaaccatt tttattgtta
    24841 attcatcacc ctgcgtggcc tgctttttga ttttctctgg ggccacggta gctgcgcgat
    24901 aatccgccac ggcggcgcag ccgataaaaa tattttgctg ctgtacagaa gcattcacgg
    24961 cggcttccat ttccagcgcg gtcatcacat caacacgttt aacaaacggt ggcgtcggta
    25021 gtgaaaccgg acctgatacc agcgtgacgt tcgcgccacg acgggcagcg gcggcggcga
    25081 tagcaaaacc catcttgccg gagctgtgat tagagatata acgcacggga tcgagcggtt
    25141 cacgcgtcgg gccggcggta atcataatgt tcagatgttt caggtcgttg acgggcgaaa
    25201 aatgcgctac cgccatatcc acaatggtta acggatcgag cattcgccca ggaccgatat
    25261 caccacaagc ctgactgcca ctgtctggcc cccagatgag caaaccacgg gaagcaagca
    25321 cctctaaatt atgctgcgtg gcagcggcac ggtacatctg ctggttcatg gcggggagca
    25381 cggctacagg cgcaggtgta gccagacaaa tcgtcgatac caggtcattc gccattccgg
    25441 cagcaacacg ggcaatcaaa tctgccgtgg caggggcgag aatcactaaa tcagcccatt
    25501 tacccagctc aatatggccc atagcggctt cggctgccgg gtccagcaaa ctgtcggaaa
    25561 cgggataacc agaaaccgcc tgcaagctaa gtggggtgat aaaggctttt gccgcttcgg
    25621 tcatggctac gcggacgtcg gccccgcgat cgcgcaaacg acgcaccagt tcaggggttt
    25681 tataggcagc aataccgccg ctaacgccga gaacgatttt tttaccggcc aggctcatga
    25741 tgatttttcc tgttgggtga caccagaagt tggcgatttt atcacaatac ttttgttgtc
    25801 gtgccttcac ctgagattca ctttgcgagg cgctttccag gattgaaaac tggccgtcga
    25861 tttaacggaa cggctatgac aggatgcgag caccacaaag gaggtgaagg tgaaaaacaa
    25921 tgcacagctg ttgatgccgc gcgaaaaaat gctgaagttt ggtattagcg ccttaactga
    25981 tgtcgagctg ctggcgctat ttctgcgtac cggaacgcgc ggtaaagatg tattaaccct
    26041 ggcaaaagag atgctggaga atttcggctc tctttatggc ttgttaacct ctgaatatga
    26101 acaatttagt ggtgttcatg gaattggcgt agcgaaattt gcccagttaa aggggattgc
    26161 tgaactggcg cgacgttact acaacgtgcg gatgcgtgaa gaaagccctt tactcagccc
    26221 ggagatgacg cgggaatttt tacaaagcca gctcacgggt gaggagcggg agatctttat
    26281 ggtgatcttt ctcgactccc aacaccgggt tataacgcat agccgtcttt tttccggcac
    26341 gctaaaccat gttgaagtcc atcctcggga aattatccgc gaagcgataa aaataaacgc
    26401 ctcggcgctg atccttgcac ataatcaccc ttcgggttgt gctgaaccca gtaaagcgga
    26461 taaactcatt actgaacgga taataaagag ttgtcagttc atggatttac gcgtgctcga
    26521 ccatatcgtg attgggcgtg gagagtatgt ttcttttgcc gaacgcggct ggatttaacc
    26581 cgctatgcgc gatccttcgg gatctttgtc tgttcgggac ttgagcacat cgctgagtca
    26641 gcgtatacta cgccaccttt gagaatctcg ggtttggcat ttgggcctgg caatcgagag
    26701 ttcacataga actgcgatga ccgggctgta aagcctgacg aggcgccaat accccatacg
    26761 aagctcgagc taatttgatt tttggagaat agacatgtcc cgagtctgcc aagttactgg
    26821 caagcgtccg gtgaccggta acaaccgttc ccacgcactg aacgcgacta aacgccgttt
    26881 cctgccgaac ctgcactctc accgtttctg ggttgagagc gagaagcgtt ttgtcaccct
    26941 gcgcgtatct gctaaaggta tgcgtgtaat cgataaaaaa ggcatcgata cagttctggc
    27001 tgaactgcgt gcccgtggcg aaaagtacta agtacttaga ggaaataaat catggctaaa
    27061 ggtattcgtg agaaaatcaa gctggtttct tctgctggta ctggtcactt ctataccact
    27121 acgaagaaca aacgtactaa gccggaaaaa ctggaactga aaaaattcga tccagttgtt
    27181 cgccagcacg tgatctacaa agaagcgaaa atcaaataat tctcgctttg atgtaacaaa
    27241 aaacctcgct ccggcggggt tttttgttat ctgcttgccc ccatattgac tgcatctgtt
    27301 cattcctgga gatgctatgc ctgaattacc cgaagttgaa accagccgcc gcggcataga
    27361 accgcatctc gttggtgcaa ccattcttca tgcagtggtg cgcaacggac gcttgcgctg
    27421 gccggtttca gaagagatct accgtttaag cgaccaacca gtgcttagcg tgcagcggcg
    27481 ggctaaatat ctgctgctgg agctgcctga gggctggatt atcattcatt tagggatgtc
    27541 tggcagcctg cgcatccttc cagaagaact tccccctgaa aagcatgacc atgtggattt
    27601 ggtgatgagc aacggcaaag tgctgcgcta caccgatccg cgccgctttg gtgcctggct
    27661 gtggaccaaa gagctggaag ggcataatgt gctggcccat cttggaccgg agccgcttag
    27721 cgacgatttc aatggtgagt atctgcatca gaagtgcgcg aagaaaaaaa cggcgattaa
    27781 accgtggctg atggataaca agctggtggt aggggtaggg aatatctatg ccagcgaatc
    27841 actgtttgcg gcggggatcc atccggatcg gctggcgtca tcactgtcgc tggcagagtg
    27901 tgaattgtta gctcgggtga ttaaagcggt gttgctgcgt tcgattgagc agggtggtac
    27961 aacgctgaaa gattttctgc aaagtgatgg taaaccgggc tatttcgctc aggaattgca
    28021 ggtttacggg cgaaaaggtg agccgtgtcg ggtgtgcggt acgccgattg tggcgactaa
    28081 acatgcgcag cgggcaacgt tttattgtcg gcagtgccag aagtaattca tgcgcgccgg
    28141 atggcatacc atccggcata aacgctacgc taacttcgcc atcagcgcct gatggacatt
    28201 ctccggcagg aaatgggtga catcgccctg atggcgcgcc acctctttca ccaacgatga
    28261 agagataaac gaccactctt tcgacggcat cagaaacaca ctttccagtt ccggcattaa
    28321 gtggcgattc atatgcgcca gctgcatttc atattcaaaa tctgccaccg cacgcaggcc
    28381 acgaatcagc accgtagcgt gttgattacg ggcgaagttc gccatcaaat cactaaaccc
    28441 gaccacttcc acgttcccca gatgcgcggt tgcctgctgt gccagtgcca cacgctcttc
    28501 cagggtaaac atcggttttt tactggggct ggcggcaatc gccagaataa cgtgatcgaa
    28561 catctgcgtg gcgcgcgtca cgatatcgat atgaccattg gtaatgggat cgaaagtacc
    28621 cggataaatc gcccgttttt gcataacaac ctcaatgcgt tttcggtggc aggtaaggtt
    28681 ccagcagttg aagcagacgc tgtagcgcgc cctggttttg atacagtact tcaacggcat
    28741 gacggccata gaaactacgg taatcggcgt cggtgagtaa agaggaaacc tcttttgcaa
    28801 gcgtagtggc atcggtaacg gtaatcagcc cgcttgcctg ctccagccgc gcgcaaatgt
    28861 ctttaaagtt aaaagtatgc ggccccatca ataccggaat tgcgtgtgcg gcagcttcca
    28921 gcggattatg cccaccacgt tcaaccagtg aaccgccaac aaaggcgaga tcggcaatgc
    28981 cgtacagtaa catcaactcg cccatcgtat cgccaaccac aacctgcgtg ctggtggagg
    29041 ggacttcccc tgaagagcgt gtgatatagc ttagtccagc ctggcggaca aggttaatcg
    29101 catccgggaa gcgttctgga tgacggggta ccaggatgag caataaattc gggaattgct
    29161 gtaataaagc ctgatgtgcg gcaatgacca cgctctcttc accttcgtga gtgctggtgg
    29221 caatccatac cgggcggtgt ggtgcccact ggcggcgcag cgtcacggct ttagcagcca
    29281 actgcggcgt tacagaaata tcgaatttca ggctaccggt aacggttacc tggttatttt
    29341 tcgcgcccag cgccacaaaa cgtgcaccat cttcttcatt ttgggcggca atcagcgtaa
    29401 tacgacgcag caagcgacgg acgaatttac ccagtttggc ataacctgcg gccgagcggg
    29461 cagagagtcg cgcgttagcg atcaccagcg gaattttacg tttatgtagc gccgcaatca
    29521 ggttaggcca tagttcggtt tccataatca acaccagttt agggtcgact ttattcagga
    29581 aacggttgag tgcatcgggc agatcatacg gcagataaac gtgctgaaca tccttcccga
    29641 aagccgattg tacgcgctcc gaaccggttg gcgtcatggt tgttacggta ataggtaaat
    29701 caggataacg atgacgcagc gcgcgcacca acgggatcgc cgccagagtt tcaccgacgg
    29761 agacggagtg cagcataatg ccgcctggtt ttagcggatg gcggtaaaaa ccgtaacgtt
    29821 caccccagcg ttttcgatag gccggagcct tacgtccgcg cacccagagc cgtatccaga
    29881 tcagcggctg aataaggtag agaagggcgg tgtaaagcaa ttcgagcata gtaaatagct
    29941 gacttatgga tgtgctgggg attctatgta tttagctgtg gctttaccat tacttttccc
    30001 gtttttgact taaatagcaa tttacccgcc aattcttgct gaacatctat actttttttg
    30061 tatttttatg cgattcattg atttctgctg tacgtgcagg aaggaatcgc agagcaacag
    30121 tgaaagaata tggttgctaa tgattttatt tgattgattt ctttattgaa aatcaacaat
    30181 gtgcttaaaa atcattcggg tcggattgcc gcgaaagaag tgatacacta gcgcgtcaat
    30241 atcagtgcgg tatcagtatt caggtagctg ttgagcctgg ggcggtagcg tgctttttct
    30301 gcttcacctt aatcggataa tctcaacaaa aagagttact tgtggataag ccatttcgaa
    30361 gaattttgct cattaagatg cgttttcatg gggatatgtt attaactact cccgtcatta
    30421 gttcgctgaa aaaaaattac cctgacgcaa aaatcgatgt gctgctttat caggacacca
    30481 tcccgatcct gtctgaaaat ccagagatta acgcgctcta cggcataaaa aataaaaaag
    30541 caaaagcctc agaaaaaatt gccaactttt ttcatctcat caaggtatta cgtgccaata
    30601 agtatgacct tatcgtcaat cttaccgatc aatggatggt tgctatactg gttcgcttat
    30661 taaatgcccg tgtgaaaatt tcccaggatt atcatcatcg gcagtctgct ttttggcgta
    30721 aaagtttcac ccatttggtg ccgttgcagg gtggaaatgt ggtggaaagt aacttatccg
    30781 tgctgacacc attgggactt gattcgttgg tgaagcagac aaccatgagt tacccgcctg
    30841 caagctggaa acgtatgcgt cgcgaacttg atcacgctgg tgttggacaa aattatgtgg
    30901 ttatccaacc tacggcgcgg caaatcttca aatgctggga caacgccaag ttttccgctg
    30961 tgattgatgc cttacatgct cgtggttatg aagtcgttct gacgtccggc ccagataaag
    31021 acgatctggc ctgcgtcaat gaaattgcgc aaggatgcca gacgccacca gtaacggcgc
    31081 tggctggaaa ggtgaccttc ccggaacttg gtgcgttaat cgatcatgcg cagctgttta
    31141 ttggcgttga ttccgcaccg gcgcatattg ccgctgcagt taatacgccg ctgatatcgc
    31201 tgtttggtgc gacagaccat attttctggc gtccttggtc aaataacatg attcaattct
    31261 gggcgggaga ttaccgggaa atgccaacgc gcgatcagcg tgaccgaaat gagatgtatc
    31321 tttcggttat tccggcggca gatgtcattg ctgctgtcga taaattactg ccctcctcca
    31381 cgacaggtac gtcgttatga tcgttgcttt ttgtttatat aaatattttc cctttggcgg
    31441 tttgcagcgc gattttatgc gtattgctca gacagtcgcc gcccgaggtc atcatgttcg
    31501 ggtttatacc cagtcgtggg aaggcgaatg ccctgatgta tttgaactga tcaaagtgcc
    31561 ggttaaatcg cataccaatc acgggcgcaa tgcggagtat tttgcctggg tgcaaaaaca
    31621 tttacgcgaa catcccgtcg ataaagtcgt tggattcaac aaaatgccgg ggctggacgt
    31681 ttattatgcc gctgatgttt gttatgccga gaaagtcgcg caggaaaaag gctttttcta
    31741 tcacctgacg tcacgttatc gccattatgc cgcctttgag cgggcaacct tcgaacaggg
    31801 caagccgaca cagctgctga tgctgacaga taagcaaatc gccgatttcc agaaacatta
    31861 tcagactgaa gcggagcgtt ttcatattct accaccgggg atttatcctg atcgtaaata
    31921 tagccagcag ccagctaata gccgtgaaat cttccgtaag aagaatggaa taaccgaaca
    31981 acaatattta ttgttgcagg tcggttcaga cttcacgcgt aaaggtgtcg atcgttccat
    32041 tgaagcactt gcttcgttac cggattcgct gcgccacaac acattgctat atgttgttgg
    32101 acaggataaa ccgcgaaaat ttgaggcgct ggcagaaaaa cgcggcgtgc gcagtaatgt
    32161 tcacttcttc tcggggcgca acgatgtctc ggaattaatg gcggcggcgg atttattact
    32221 gcatcctgcc taccaggaag cagcgggaat tgtgctactg gaagcgatta ctgcaggatt
    32281 accggtacta acaacagccg tttgtggcta tgcgcattat attgtcgacg ccaattgcgg
    32341 cgaggctatt gctgagccat tccgccagga aacattgaat gagattttac gcaaagcgtt
    32401 aacgcaatct tcattgcgcc aggcctgggc ggaaaatgcg cgacattatg ctgatacaca
    32461 agatttatac agtctgccag agaaagcggc ggatatcata acgggtggtc tggatggttg
    32521 aacttaaaga gccgtttgcc acgttatggc gcggtaaaga tccttttgag gaagttaaaa
    32581 ccttgcaggg tgaggttttt cgtgaactgg aaactcgccg cactctgcga tttgaaatgg
    32641 cgggcaaaag ctattttctc aaatggcatc gcggcacgac cctgaaagag ataatcaaaa
    32701 atttactctc attgcggatg ccagtattag gcgcagaccg cgaatggaat gcgattcatc
    32761 gactgcggga tgtcggcgtt gatactatgt atggggtggc atttggcgaa aaaggcatga
    32821 atccgctgac cagaacttca tttattatta ccgaagatct gacaccaacc ataagtcttg
    32881 aagattactg tgctgactgg gcgactaacc ctccagatgt tcgcgtaaag cgtatgctta
    32941 ttaagcgtgt cgcgacgatg gtgcacgata tgcatgctgc gggcattaac caccgtgact
    33001 gttatatctg tcatttcctg ctgcacttgc ctttttccgg taaggaagag gagttaaaaa
    33061 tttcggtaat tgacctgcac cgggcgcagc ttcgcacgcg cgttccacgt cgttggcggg
    33121 ataaagatct tattgggctt tatttttctt cgatgaatat cggcctgact cagcgggata
    33181 tctggcggtt tatgaaagtg tattttgccg ccccgcttaa agacattctc aagcaggaac
    33241 aaggactgct gtcgcaagca gaagcaaaag ccacaaaaat cagggaaaga acgattcgaa
    33301 aatcgttgta acccgtaact tatttttgcc aaaattttgg atacagaata aatatgtctc
    33361 aactcaatga tagtgacatc atcctttttg agtataattt tcattatcaa aatataagat
    33421 ctaaaaatac tcttgatata gcatttggta ttgacagaaa ttttcttttt ggatgtggtg
    33481 tagccatcgc atctattcta ttaaacaata gagaaatctc tt
//
LOCUS       DAZFDC010000041        32522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFDC010000041 DAZFDC010000000
VERSION     DAZFDC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.041"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..296)
                     /locus_tag="ACHJ48_002037"
     CDS             complement(<1..296)
                     /locus_tag="ACHJ48_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707598.1"
                     /translation="MGNIHGISCTANNYLKQAWNDIKNEYEKNKTYSITLFENTLVCF
                     MRLYKEIRRQQAEDYIPCLECDSLEKEFEEIQNDNDLSLFMKTLRTNDKQIYSGV"
     gene            556..1476
                     /gene="cbpA"
                     /locus_tag="ACHJ48_002038"
     CDS             556..1476
                     /gene="cbpA"
                     /locus_tag="ACHJ48_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO4707599.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1476..1781
                     /gene="cbpM"
                     /locus_tag="ACHJ48_002039"
     CDS             1476..1781
                     /gene="cbpM"
                     /locus_tag="ACHJ48_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO4707600.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1935..2534)
                     /gene="torD"
                     /locus_tag="ACHJ48_002040"
     CDS             complement(1935..2534)
                     /gene="torD"
                     /locus_tag="ACHJ48_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO4707601.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPTDHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2531..5077)
                     /gene="torA"
                     /locus_tag="ACHJ48_002041"
     CDS             complement(2531..5077)
                     /gene="torA"
                     /locus_tag="ACHJ48_002041"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO4707602.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTSRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWHDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKCNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(5077..6249)
                     /gene="torC"
                     /locus_tag="ACHJ48_002042"
     CDS             complement(5077..6249)
                     /gene="torC"
                     /locus_tag="ACHJ48_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO4707603.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRAGANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6379..7071
                     /gene="torR"
                     /locus_tag="ACHJ48_002043"
     CDS             6379..7071
                     /gene="torR"
                     /locus_tag="ACHJ48_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO4707604.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(7044..8072)
                     /gene="torT"
                     /locus_tag="ACHJ48_002044"
     CDS             complement(7044..8072)
                     /gene="torT"
                     /locus_tag="ACHJ48_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO4707605.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            8155..10899
                     /gene="torS"
                     /locus_tag="ACHJ48_002045"
     CDS             8155..10899
                     /gene="torS"
                     /locus_tag="ACHJ48_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO4707606.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFIQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDVN
                     GITLARQLARQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANNDLPLDVSQLNEDAQLMGTEKIHEWLALFTQHALPLLDEIDIARATQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            10971..12044
                     /gene="yccM"
                     /locus_tag="ACHJ48_002046"
     CDS             10971..12044
                     /gene="yccM"
                     /locus_tag="ACHJ48_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO4707607.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWHNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTIFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(12093..12227)
                     /locus_tag="ACHJ48_002047"
     CDS             complement(12093..12227)
                     /locus_tag="ACHJ48_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO4707608.1"
                     /translation="MADFIAQKIAELNKNTGKEVSEIRFTAREKMTGLESYDVKIKIM
                     "
     gene            complement(12255..12485)
                     /gene="ymcE"
                     /locus_tag="ACHJ48_002048"
     CDS             complement(12255..12485)
                     /gene="ymcE"
                     /locus_tag="ACHJ48_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO4707609.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12460..12648)
                     /gene="ymcF"
                     /locus_tag="ACHJ48_002049"
     CDS             complement(12460..12648)
                     /gene="ymcF"
                     /locus_tag="ACHJ48_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO4707610.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12659..12871)
                     /gene="cspG"
                     /locus_tag="ACHJ48_002050"
     CDS             complement(12659..12871)
                     /gene="cspG"
                     /locus_tag="ACHJ48_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO4707611.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            13157..13369
                     /gene="cspH"
                     /locus_tag="ACHJ48_002051"
     CDS             13157..13369
                     /gene="cspH"
                     /locus_tag="ACHJ48_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO4707612.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            13811..14116
                     /locus_tag="ACHJ48_002052"
     CDS             13811..14116
                     /locus_tag="ACHJ48_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707613.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            14223..14867
                     /gene="gfcB"
                     /locus_tag="ACHJ48_002053"
     CDS             14223..14867
                     /gene="gfcB"
                     /locus_tag="ACHJ48_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO4707614.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            14864..15610
                     /gene="gfcC"
                     /locus_tag="ACHJ48_002054"
     CDS             14864..15610
                     /gene="gfcC"
                     /locus_tag="ACHJ48_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4707615.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGNYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVIVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            15610..17706
                     /gene="gfcD"
                     /locus_tag="ACHJ48_002055"
     CDS             15610..17706
                     /gene="gfcD"
                     /locus_tag="ACHJ48_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4707616.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            17752..18891
                     /gene="gfcE"
                     /locus_tag="ACHJ48_002056"
     CDS             17752..18891
                     /gene="gfcE"
                     /locus_tag="ACHJ48_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO4707617.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            18879..19325
                     /gene="etp"
                     /locus_tag="ACHJ48_002057"
                     /gene_synonym="yccY"
     CDS             18879..19325
                     /gene="etp"
                     /locus_tag="ACHJ48_002057"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO4707618.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            19345..21525
                     /gene="etk"
                     /locus_tag="ACHJ48_002058"
     CDS             19345..21525
                     /gene="etk"
                     /locus_tag="ACHJ48_002058"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO4707619.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTITGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(21645..22949)
                     /gene="appA"
                     /locus_tag="ACHJ48_002059"
     CDS             complement(21645..22949)
                     /gene="appA"
                     /locus_tag="ACHJ48_002059"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO4707620.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPGQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMIALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(23029..23121)
                     /gene="cbdX"
                     /locus_tag="ACHJ48_002060"
     CDS             complement(23029..23121)
                     /gene="cbdX"
                     /locus_tag="ACHJ48_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO4707621.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(23134..24270)
                     /gene="appB"
                     /locus_tag="ACHJ48_002061"
     CDS             complement(23134..24270)
                     /gene="appB"
                     /locus_tag="ACHJ48_002061"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO4707622.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLSTKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(24282..25826)
                     /gene="appC"
                     /locus_tag="ACHJ48_002062"
     CDS             complement(24282..25826)
                     /gene="appC"
                     /locus_tag="ACHJ48_002062"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO4707623.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPVLLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(25960..26817)
                     /gene="hyaF"
                     /locus_tag="ACHJ48_002063"
     CDS             complement(25960..26817)
                     /gene="hyaF"
                     /locus_tag="ACHJ48_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO4707624.1"
                     /translation="MSETFFHLLGPRTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(26814..27212)
                     /gene="hyaE"
                     /locus_tag="ACHJ48_002064"
     CDS             complement(26814..27212)
                     /gene="hyaE"
                     /locus_tag="ACHJ48_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO4707625.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(27209..27796)
                     /gene="hyaD"
                     /locus_tag="ACHJ48_002065"
     CDS             complement(27209..27796)
                     /gene="hyaD"
                     /locus_tag="ACHJ48_002065"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO4707626.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPESVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(27793..28500)
                     /gene="hyaC"
                     /locus_tag="ACHJ48_002066"
     CDS             complement(27793..28500)
                     /gene="hyaC"
                     /locus_tag="ACHJ48_002066"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO4707627.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(28519..30312)
                     /gene="hyaB"
                     /locus_tag="ACHJ48_002067"
     CDS             complement(28519..30312)
                     /gene="hyaB"
                     /locus_tag="ACHJ48_002067"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO4707628.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(30309..31427)
                     /gene="hyaA"
                     /locus_tag="ACHJ48_002068"
     CDS             complement(30309..31427)
                     /gene="hyaA"
                     /locus_tag="ACHJ48_002068"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO4707629.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATSIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            31853..31940
                     /locus_tag="ACHJ48_002069"
     tRNA            31853..31940
                     /locus_tag="ACHJ48_002069"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31887..31889,aa:Ser,seq:tga)
BASE COUNT         7962 a         8551 c         8266 g         7743 t
ORIGIN      
        1 acccctgaat aaatttgttt atcattagta cgcaatgttt tcataaataa tgataaatca
       61 ttatcattct gtatttcctc aaattctttt tctagtgaat cgcattccag gcatggaatg
      121 tagtcttcag cttgttgacg tctgatttct ttatacaacc gcataaaaca caccagtgtg
      181 ttttcaaaaa gcgtgattga atatgtttta tttttttcgt actcattttt tatatcgttc
      241 caggcttgct tcaaataatt atttgcagtg caactaattc catgtatatt acccatatat
      301 agcgcctata aaatttaata aataataacg cactagtaaa acttaaagtg cctgtttcaa
      361 ctatcaaaaa tcgctcaccc tttttcacct gtttaaaata tgttcagcgc cccaccttga
      421 ggcgacctcc tctccgcgat gatttcaata acatattctg tgttggcata tgaaattttg
      481 aggattaccc tacacttata ggagttacct tacaggggtt ccttcaattt gtgttgattt
      541 acgcgagata acgctatgga attaaaggat tattacgcca tcatgggcgt gaaaccgacg
      601 gacgatctca agacaatcaa gaccgcctat cgtcgacttg cccgcaaata ccatcctgat
      661 gtcagcaaag aaccggatgc cgaagcccgc ttcaaagagg tcgctgaagc ctgggaagtg
      721 ttaagtgatg aacaacgtcg cgctgagtat gatcagatgt ggcaacatcg caacgatccg
      781 caatttagcc gtcagttcca gcatggcgac ggtcagagtt ttaacgccga agattttgac
      841 gatatcttct cgtcaatttt cggtcagcat gcccgccaga gccgtcaacg ccccgccgca
      901 cgcggccacg atattgaaat cgaagtggcg gtattcctcg aagaaacgct tactgagcat
      961 aagcgtacca tcagctataa cctgccggtt tataacgcct ttggcatgat cgaacaggaa
     1021 atcccgaaaa cgctgaatgt gaagatcccg gcaggcgtcg gcaatggtca acgcatccgc
     1081 ctgaaaggcc aggggacgcc gggcgaaaac ggcggtccaa atggcgattt gtggctggtg
     1141 attcatattg cgccacatcc gctgtttgat attgtcggcc aggatctgga aattgtggtg
     1201 ccggttagcc cgtgggaagc ggcgctgggt gctaaagtca ccgttccaac actgaaagaa
     1261 agcattttgc tgactatccc gcctggcagc caggccgggc aacgattgcg cgttaaaggc
     1321 aaaggtctgg tgagcaaaaa acagaccggc gatctgtatg cggtactgaa aatcgtgatg
     1381 ccgccgaaac cggatgaaaa cactgccgcg ctgtggcagc aactggcaga cgcccagtcg
     1441 tcttttgatc cacgtaaaga ttgggggaaa gcataatggc taatgttacg gtgactttta
     1501 ctattaccga attttgcctg cataccggca tctctgaaga ggaattgaat gaaattgtcg
     1561 gtttgggggt ggttgaaccg cgtgagatcc aggaaacaac ctgggtattt gacgaccatg
     1621 ccgccatcgt ggtgcaacgc gcggtgcgcc tgcgtcatga actggctctg gactggccgg
     1681 ggatcgcggt ggcgctgacg ttaatggatg atattgcgca cctgaagcag gaaaaccgcc
     1741 tgctgcgcca gcggctttcc cggtttgtag ctcatccgtg atgtgtgttt gccggatgtg
     1801 atgacgcatc ttagcctgcc tgcaaagagt acttttcctg tgggccggat aaggcgtgtt
     1861 cacgccgcat ccggcggcgg tgccctaatg cctgatgcga cgctaaacgc gtcttatcag
     1921 gctaacaaac gacgttatct gttttggtgg tcgccctcaa ctaacaccag caataactgg
     1981 cttagtgccg cgtaaaaacc aaagctgtca tactgatggc aacgcgcaac aaactctggt
     2041 aaccattgcc gcaacgccgt gagtgttttt tgccgtaaac tgtcgattct tcgcgcagga
     2101 accgtcccct ctcccagcga aaaatgcaga tgactcaaca actcgagata gatcgccaga
     2161 tgatctgtcg gttcgttgaa attgccgctg gtttccatcc ctgcctcaac taacaagcgt
     2221 ttaatctctt gctcgtcctg tttgtaggcc gatgcatacg gcagcgccgc ttgtttgtca
     2281 gtcatcagaa acaggccgca aaagtcagcg gccagttcca gacgggcatc gtcacgtact
     2341 gtcagagtgg caacacggtt ttccagctca tccaccgccg cagtgagcgg cggttcgctt
     2401 ttcagcaacg aaaaccattc agccatctgc gcactggcga tttgcgtcag ttgttcatcg
     2461 tccagttcac gggagaacaa ctgcgccagc caggcgtaaa cacaggctat ctgttgtgct
     2521 gtcagcgtgg tcataatttc acctgcgacg cgggaacata ttcgcactgc gccaccatct
     2581 ccacggggcc gttaaacgcc gttacctgct ccactgttcc gttgcacttc tcaatttcca
     2641 ccagcgtagt gtgcgcactg gtcgcctgcg ccagctgcga agtaccgatg tcaatggtca
     2701 acacgttggg gttaccgtat ttgcacagcg caccaggctc gccgccttta tctggatcgt
     2761 gccatgcccc ttcgtgaatt cgcgccacgc cgggtgcata gcggtcagaa actactgccc
     2821 ctgccaacac ctgaccgcga gcgttaaaga cgcgtaccac atcaccgtta cgaataccgc
     2881 gcgcgctggc atcctgcggg ttaatgaaca ctggctcttt acccgctacc gtatattgct
     2941 gacgcagagt ttccgactca cataactgcg agtgaagtcg gaaatccgga tgcacagatt
     3001 gcagatgcaa cggatacgtc tgcgagccgg gcccaccgtg ggagcgttcg attttctcaa
     3061 accacatcgg atgcccctga caatcgtcgt agttcatatc ggcgatggtt ttcgaataga
     3121 tctcaatcag gccactcggc gtgcccagcg gttcgagatc tggatcttcg cggaatgcct
     3181 ggtggcgaac aaacatctgc ggatggtcaa actcgacgta ctctttgtta ttccagaagt
     3241 catcaaacgc tggcaaatga acgccgcgtc ctttgccttg ctgaacacct tcctgccaga
     3301 tgcgtttcag ccagcccatt tcgtccagcc cttcggtaaa ggcttcttcg cgattaaagc
     3361 ggcggcacag ctcgcggaaa atatcaaagt cgttgcgcgc ctcgaactgc ggcggcacga
     3421 cctgtttcat ggcgataatg ccacggttgg agtggttgcc gtactggtcg agatcgttac
     3481 gctcaaactg cgtggtcgca ggcagcacga tatcggcaaa gcggcaggtt gaggtccact
     3541 ggttatctat ggcgataacc gtttccagtt tgcgccagcc ttcaataatg cggttgatct
     3601 gctgatggcg atggaagggg ttagttccgg caaaaataca cattttcagc ggcggcagtt
     3661 ttaccgattt accgttccag ttgattactt tccccggttc gaggatcgca tcgataaaac
     3721 gggcaatcgg aatggtgctg ctgtagcctt tgtaatcact gttgtcgtga acaggcggaa
     3781 tcgacgtaga gccggagaaa ccactcagaa taacgccttt acgccccggc gtgcctgcgc
     3841 cgttatagtg ccagccaaaa ccaaagccac cacctggcag gccaatttgc cccagcatcg
     3901 ccgccagaac cacaatcatc cacgcccact gttcaccgtg ctgcatacgc tgtacgcacc
     3961 agccagcaat aatctgcgtt ctgttcgccg ccatctgccg cgccagtcca cgaatggttt
     4021 cggcatcaat gccggtcagt ttttcagccc atgcggcatc tttcggctga ccgtctttct
     4081 cacccagcag atacggcagg aactgctcaa aacccacaca gtagttagcg aggaagtttt
     4141 tgtcgtacag gttttcactg tacaacgtat gcgccagcgc cagttgcagc ggcacgtcag
     4201 tttgcgggtt aaccgcaatg tgcttcacat gctcgcgccc cagatactca tgggtggatg
     4261 taacaaccgg atcgatgcta atgacctcaa tttcaccggc ggcgactttc gctttcaact
     4321 gcgcgtaata ttcataaaca tcgtgatccg ggcaccacca gttcgcttgc tggtttttca
     4381 gcaaatcgga gccccacagc acaatggttt tgctgttctg caataccagc ggccaggagg
     4441 tttgctgttc atacacttcc atcgaaccga ctacgcgcgg caggatcacc tgcgcagcac
     4501 cagtagagta atctccgccc gtaccaacgc tattaccatg caaggcaata gctttcgcca
     4561 gcatccccga agcgttatgg aacatccccg tcgattgcca accactggcg gtcagcaagg
     4621 cactcggccc gtgagttttc tgtacgcgtt ccagttcttc atagaacatg tcgagggctt
     4681 catcccagct cacacgcaca aaacggttat caccgcgctg ggaggtgtcg ctcagatggc
     4741 gcttacgcag ccagtccacg cgtaccatcg gataacgaat acgcgccgcg ttgtgtacat
     4801 gatccggcaa tccggcaatc attttcgacg gatatttatc cagttcgaac ggttttgccg
     4861 ccacaaagcg accatccttc accgtcgcgc ggatagcccc ccagtgcgac ccggtaagaa
     4921 tgccctcttt cgagatgaca gcctcagtcg ccgcttgcgc cgcagtcgca cggcgcgacg
     4981 ttaacaatga cggccccagc atcccggcga cggttaagcc gccgagttgt gccagaaaac
     5041 gccgacgtga tgcctgaaag agatcgttat tgttcattat ttttcttcct tcttatcgcc
     5101 gtgagcctta cctgcggtgt cagacgcatt catttgcaga tatttcaaca aggtgcgttc
     5161 ttcacgttta tcgaggctgg taaagccgat catgccgttg agcgtgccaa tccagccgtt
     5221 agcgtcaaag tgagagattt ccggtgcgcc gtggcactgg ttacaggtgc cgttgtacaa
     5281 cgaatccgca taagcccaga tcggtttgat atcgttcacc atgtcacctt tcttcatcca
     5341 cgcggtggcc tgcaacttgc tccactcggt attggtgtcg gcaacggtgg ttttctccag
     5401 cgtttttacc tgctgctgca catcaccacg aatcgaggca acaaagatgc gtttacctgg
     5461 gaattgggtg agtacacgct gacgtccggc gctttctgtc cagccggtaa tttcaatttg
     5521 cagccagtcg ccgtcacgtt taaggacttt cacttccgag gcaggcagca gagaaccaga
     5581 ggcttcttta tcgcctttcg ccgcataaat tggcttaata tcaatagagt acagcgtgtc
     5641 accactgtca ttagcaccgg cgcgcagctc atcgaactgc ttacggaagc cgctactcat
     5701 atccggtaac tggtgggcaa tacctttatg acagtcgatg caggattgat tatctttcgc
     5761 tgccaccttc atctgacgtg ccgcttcagg atgctgcttc gcatgatcca tcgcatcgta
     5821 gttatggcag gagcgacagg ttgccgagtt gttttctttc attcgcgccc attcacgctc
     5881 ggcaagttcc gcgcgtttgg cttcaaattt ttcaggtgta tcaatggagt gggcaataaa
     5941 ggtctggtag atatcgttgc tcgcttccag tttgcgcttc accatgcctg gaatatccgg
     6001 cgggatatga cagtcatggc attcagctcg cacgccggag gcgttctgga aatgcaccga
     6061 ctgtttatat tcttcataca ctggttgcat actgtggcaa ctgacacaaa attcggttgt
     6121 gctggtgact ttgatcccaa cgtgtggcaa tacaatcagc gcaatgccaa tcacaatccc
     6181 aatagcgacc agcgccagta ccgaccaacg agcactgggt cggcgtagcg cgttccagag
     6241 tttccgcata atagcccctg taaaattatg gtttagtgaa gcgatcttaa tgagtaaata
     6301 tgaacagcgg cactggtcag gatgaacggc ttacggcaga atatgaacag atatgaacag
     6361 aatgagtaaa accctctgat gccacatcac attgttattg ttgaagatga gccggttacc
     6421 caggcgcgat tacaatccta cttcactcag gaggggtata ccgtttccgt tacggcgagc
     6481 ggtgccgggc tacgggaaat tatgcagaat cagccggtgg atttaattct gctggatatc
     6541 aacttgcccg atgaaaatgg cctgatgtta acccgcgccc tgcgagaacg ctcaacggtg
     6601 gggattattc tggttaccgg acgcagcgat cggattgacc gtattgttgg gctggaaatg
     6661 ggcgcagacg attacgtcac caaaccgctg gaactgcgcg aactggtagt acgggtaaaa
     6721 aatctgctct ggcgaatcga cctcgcgcga caagctcaac cgtacactca ggacaactgc
     6781 tatcgctttg cgggttattg cctgaatgtg tcgcgccata cgctggagcg ggatggtgag
     6841 ccgattaaac tgacccgcgc agagtatgaa atgttggtgg catttgtgac caatccgggc
     6901 gaaattctca gccgtgaacg tctgctgcgt atgctctctg cgcgtcgggt ggaaaaccct
     6961 gacctgcgca ccgtcgatgt gttaattcgt cgtttacgcc ataaactcag cgcggattta
     7021 ctggtgacgc aacatggcga aggttatttc ttagccgctg atgtgtgctg ataaaaatag
     7081 accggacgaa atcccccagg cgacagcgag cggcggatat gttcgcggtc ggcatttttc
     7141 ggcgtcagaa ctaaaatcgg tgggctgaca ttatcagaca ccgattgccc ctgtaattgc
     7201 ctgatggcct gctcaactgc cagttccccc tgccagacca tttgatcgct ggcagccata
     7261 atcactcttc cccgcttcag cccgcgatac acctgatgtg aaagataaaa cgacaccacg
     7321 gtaagcggcg ttttcaggtt acgcccttcc cccattgccg cctctgccgc aatggccgtt
     7381 ccggcaacga cgtcgatttc tggatggcgt tccagcatct cctgcaacag gttacgctgg
     7441 atttcaatat cgttatcacc aagcgcaata tcaacaatac gcaccgggct tccggcaatg
     7501 gctgcgcgaa aaccctcgac catctcctta ctgcccccgg cgttatcggg tccgggcatc
     7561 aacagcacat tcagtggttt accgtgcgcc cattgcacca gatatcgccc aggttgatag
     7621 cccatctgaa accagggcac accaacgcgg cttttcacct ggggagcatc aatagcattt
     7681 accagctcga tcaccggcag atttgctact tgcttttgca gatcgggaaa tgacgtcgtg
     7741 ctactaccga gcaaaatggc ctctgcgccc cactgtttac actggtcgat ttgtgcttgc
     7801 tgggtagcca actggctgta gccgcctgcc tccagcactt ttaaatccac accgtagcgg
     7861 cgagcagcct cctgcatacc atagttcaac gataaccaat atgaatcttt caggctggga
     7921 taaagcgcgc acagtttcca tacgcgtttg gcttttagcg gcatagaggc ttgcaccgtg
     7981 aaatgctgcg catcatgcca gcgcaacagg ttatcagccg aaaatgccgg caacatgaaa
     8041 agggaaagaa gtaaaaatag cagcacgcgc atgatagcct catcaataat aaggctttat
     8101 gctagatgca ttccgctttg cgactcaaca tttttcacct taagtgcacc gaccgtgaat
     8161 ttaaccctga cccgaagact ctggatgggc tttgccctga tggcgctgtt aaccctgacc
     8221 agtaccctgg tgggatggta caacctgcgc tttatcagcc aggtggaaaa agacaatact
     8281 caggcattga ttcctaccat gaatatggcg cgccagttga gcgaagccag cgcctgggaa
     8341 cttttcgccg cgcagaacct gaccagtgcc gataacgaaa agatgtggca ggcgcagggg
     8401 cgaatgctca ccgcacaaag cctgaagatt aatgcgttgc tgcaagcgtt acgggaacaa
     8461 ggttttgata ccaccgctat tgaacaacag gagcaggaga tctcccgttc gttacgtcag
     8521 caaggggaac tggtggggcg gcgtctgcaa ctgcgccagc aacaacagcg actcagtcag
     8581 cagatagtcg ctgccgccga tgagatcgca cgcctggcgc aaggtcaggc gaataatgcg
     8641 gcaacctccg ccggagcgac ccaggccggg atttacgatt tgatcgaaca agatcagcgt
     8701 caggctgctg aaagtgcact cgatcggctg attgatatcg atcttgagta tgttaaccag
     8761 atgaatgaac tgcgccttag cgctctgcgg gtgcagcaaa tggtgatgaa tctggggctg
     8821 gagcagatcc agaaaaatgc gccaacgctg gaaaagcagc tcaataatgc ggtgaaaatt
     8881 ctgcaacgtc ggcaaatacg cattgaagat ccgggagttc gtgctcaggt cgcaacaacg
     8941 ttaactaccg ttagccaata tagcgatttg ctggcgctgt tccagcagga cagtgaaatc
     9001 agcaatcacc tacaaactct cgcacaaaat aacatcgccc agttcgcgca gtttagcagc
     9061 gaagtcagtc agctggtcga caccattgag ctgcgtaatc agcacggact ggcgcatctg
     9121 gaaaaagcca gtgcacgcgg gcaatacagc ctgttattgc tggggatggt ttcactttgc
     9181 gcactgattc tgatcctctg gcgcgtggtt tatcgctcag tcacgcgtcc acttgccgaa
     9241 caaacgcagg cgctgcaacg gctgctggac ggtgatattg actccccttt cccggaaacc
     9301 gctggcgtac gggagctgga caccatcggg cggctgatgg atgcgtttcg cagcagtgtt
     9361 catgcactga atcgccaccg cgaacagctg gcggcgcagg tcaaagcgcg tacagctgaa
     9421 ttgcaggaac tggtgataga acaccgacaa gcacgggcgg aagcagaaaa agccagccag
     9481 gcaaaatcgg cgtttctggc ggcgatgagc catgagatcc gcacaccgct gtacggtatt
     9541 ctcggcaccg cacagctact ggcagataac cccgcactta acgcccagcg tgatgatttg
     9601 cgggcaatta ccgactccgg cgaatcgttg ctgaccatcc tcaacgatat tctcgattat
     9661 tccgctatcg aagcaggtgg caagaatgtt tcggtcagcg atgaaccctt tgaaccgcga
     9721 ccgctgctgg aaagtaccct gcaattaatg agtggacggg tgaaaggtcg cccgattcgc
     9781 ctggcaacgg caattgccga cgatgtaccg actgcgttaa tgggcgatcc gcggcgtatt
     9841 cgtcaggtca ttaccaacct gttgagcaac gccctgcgtt ttactgacga agggcagatc
     9901 gttttacgta gccgtactga tggtgagcaa tggctggtcg aagtggaaga cagcggctgc
     9961 ggtattgatc ccgcgaaact ggcagaaatc ttccagccat ttattcaggt aagtggcaaa
    10021 cgcggcggca ccgggctggg gctgactatc agtagccgtc tggcccaggc gatgggcggc
    10081 gaactgagcg ccaccagcac gccggaggtt ggaagctgtt tttgtttacg cttgccgtta
    10141 cgtgttgcca cggcacccgt gccaaaaaca gtcaatcagg cggtgcgtct tgacggttta
    10201 cgtttgctgt taattgaaga taacccgcta acccagcgaa ttaccgttga gatgctgaac
    10261 accagtggtg cgcaggttgt tgctattggc aatgccgcgc aggctttaga gacactgcaa
    10321 aatagcgaac cgtttgctgc cgcactggtg gattttgatc tgccggatgt caacggcatt
    10381 acgcttgccc gacaactggc acgacaatat ccgtcgctgg ttttgattgg ctttagcgcc
    10441 catgtcattg acgaaacgct gcgccagcgt accagttcac tatttcgcgg gattatccct
    10501 aaaccggtgc cgcgtgaagt gctcggtcag ttactggcgc actatctcca actgcaagcc
    10561 aataacgatc taccactgga tgtatcgcaa ctcaatgaag acgctcagtt aatggggacg
    10621 gagaagatcc acgaatggct ggcattattt acacaacatg ccctgccgct tctcgatgaa
    10681 atcgacattg cccgcgccac tcaggatagc gagaaaataa agcgtgccgc gcatcagcta
    10741 aaaagcagtt gctcgagtct gggaatgcgt agcgccagcc agctctgcgc acaactggag
    10801 cagcagccat tatctgcccc ccttccacac gaagaaatta cacgcagtgt tgccgctctg
    10861 gaagcatggt tgaataagaa agacctgaac gcgatttgaa tcaatatcag ccagttaaga
    10921 ataactagaa tagtggcatc tggtatgcaa cttaacggac tggcaaaatc atggcagaga
    10981 aaaaaagaac ccgctggcag cggcggccag gcacgacggg cggcaaatta ccgtggaatg
    11041 actggcacaa tgccacgacc tggcgtaaag cgacgcaatt attgctgttg gcaatgaata
    11101 tttatattgc catcacgttc tggtattggg tgcgctatta cgaaacggca ggtagtacga
    11161 tatttgtcgc cagaccggga ggtatcgaag gctggctacc gattgccggt ctgatgaatc
    11221 tgaaatatag ccttgcaaca ggtcagttac cgtccgtcca cgccgccgcg atgctgttat
    11281 tggtcgcttt tatcgtcatc agtctattac tcaaaaaggc cttttgctca tggttatgcc
    11341 cggttggtac gctttctgaa ttaatcggcg ctctcggtaa caaactgttt ggtcggcaat
    11401 gtgtccttcc ccgctggctg gatattcctc tgcgcggtgt aaagtatttg ctgttgagtt
    11461 tttttctcta tatcgcgtta ttgatgcctg ctcaggcgat tcactatttt atgttgtcgc
    11521 cctacagcgt ggtgatggac gttaaaatgc tcgatttctt tcgtcatatg gggaccgcga
    11581 cattaatcag cgtgaccgtt ttgctgattg ccagcctgtt tattcgccat gcctggtgtc
    11641 gttatctttg cccatatggc gcgctgatgg gcgtggtttc gctattatcg ccgtttaaga
    11701 ttcgtcgcaa tgccgaaagt tgtatcgatt gtggcaaatg cgcaaaaaat tgcccatcac
    11761 ggatcccggt cgataaatta attcaggtac gaacagtgga atgtaccggc tgtatgactt
    11821 gcgtagagtc atgtccggta gcctcaacat tgaccttttc actgcaaaaa cctgcggcaa
    11881 ataaaaaagc ctttgcgttg tctggctggt taatgacgct actggttctg gggattatgt
    11941 ttacggtgat tggttacgca atgtatgcag gagtatggca aagcccggtg ccggaggaat
    12001 tgtaccgacg cttaattccg caagcgccaa tgattggtca ctaaattagc aacaagcgga
    12061 acatcacctc gcacggggtg atgttcacaa aatcacatta ttttgatttt gacatcataa
    12121 ctttcaagcc cggtcatttt ttctcgcgcg gtgaagcgaa tttcagagac ttctttccct
    12181 gtattcttgt tcagctcggc gattttttgc gcgataaagt cggcgatttc cgtttcggct
    12241 tgttttttaa ctcttcaata ttcactgtta acctcttctg gtcttaaaag acagcaacac
    12301 gccagtaagg tgttggttcc cggtaccctg gtcaggtaag aaatgttata agccatctcc
    12361 ccgaaagatt caataatcaa caggttatcg cttactatac atacagcgtt gaaaaaaaat
    12421 aagacagata taaaaaaggc tccacaaggg agcctgatat tattttgtga aatccagcgg
    12481 tgcatggtca gtgcgtattt gcagcgcgac gttatcaaag gtgatcatcg tcgatttaca
    12541 aaaaatacac ttagcgccca aagggtttcg ctccgtcaca tcgaatgccg atgtgcggta
    12601 ctgtgaacca tgacaacatg gacagcgaaa atggagatgt tgagtaataa cggcaacctt
    12661 agagcgtaac tacgttcgct gccgcggggc cacgttgacc ctgctcaata gaaaattcaa
    12721 ctttctgatt ttcgttcagc gtgcggaatt cattgctctg gatggcggtg aaatggacga
    12781 aaacgtcttt gctgccatca tcaggagtga taaagccaaa acctttatct gcgttaaacc
    12841 attttactaa accagtcatt ttattagaca tattttactt ccttcatttt gggcctttcg
    12901 gcaaacaggg ctttaataca gaaactactt agtgttcagg agagagactc aaaagaaggg
    12961 gataatgcct gataatgaga actgctttag taaactactt tgtatgctgt ctgtctttcc
    13021 aaccgacgca gctattaacg catgataatt gagattaagc aaattttatt ttagccgtcc
    13081 ggcggtctta aatatcgctg gctttatttt attaaccccg tatagtgcag cgcagtattt
    13141 tcagtaagga atttgtttgt cccgtaaaat gacaggaatt gtcaaaacct ttgatcgcaa
    13201 aagcggcaaa ggattcatta tcccctccga cggtcgtaaa gaagttcagg tccatatttc
    13261 cgcattcact ccccgcgacg cagaagtgct tataccagga ttacgcgtgg aattttgccg
    13321 cgtaaatggc ctgcgaggac caacagcggc aaatgtttat ctctcttaat atgacgctgc
    13381 ttgtttaaag ctgactggtt ttattggcat agttcaatag tcaacaccat gtattataac
    13441 acaagttact ttttcaccgg aaaactaatc catttaaccg aatatctcta caactaatag
    13501 ttataaatgc cacttaacag cgagttatag taccacttga aactatattg ccaacaatag
    13561 ttaatagctt tcttaacaat tttcttatct ttaatttgta ttttgctaag atttatctta
    13621 tgctcacggg tcgtatttcc agaggggaat agttaacaat cttatatttc cctctgcgcg
    13681 cctttctcac ccgctgtata tagtgcaagt ggtatgagga aagggagatt tcacaaggat
    13741 gttatactca aagtagacaa tcctgatctc aagcgtacgt attgtccatg cgatgaataa
    13801 aggaaaagtt atgaaacaca agctttctgc aatccttatg gccttcatgt tgacgacgcc
    13861 agctgcgttt gctgcccctg aagcagcaaa cgggaccgag gcaacgacgg gtactactgg
    13921 caccacaacg accactaccg gtgcaaccac gacggctgct accactggtg gcgtggctgc
    13981 tggcgctgtc ggcacggcga cagttgtcgg tgtggcaacg gctgttggtg tggcgacgtt
    14041 ggcagtagtt gcagcaaatg acagtggcga tggcggttct cataacacct caaccaccac
    14101 gtccactacg cgctaattct gcttaaacgc gtcgaaatta aagttaaata atacccggtt
    14161 gcgatattcg taccgggctt tagagatttg cattttttta attaaccttt caaggattaa
    14221 aggtgcgccc tcttatttta tcgattttcg cactatttct tgcgggatgt acgcacagcc
    14281 agcaaagtat ggtcgataca tttcgcgcca gccttttcga taatcaggat atcaccgtag
    14341 cggatcagca gatccaggcg ttgccttatt ccacgatgta tttacgcctt aatgaagggc
    14401 aacgaatctt tgtggtactg ggatatatag aacaagaaca aagcaaatgg ttatcccagg
    14461 ataacgccat gctggttacc cacaatggac gtcttttaaa gaccgtcaaa cttaataata
    14521 atctgctgga agtgacaaat tccgggcagg accctctgcg taacgcgctg gcaataaaag
    14581 acggcagccg ctggacgcgc gatattctct ggagtgaaga caaccatttt cgctctgcga
    14641 ccctgagttc cactttttcc tttgctggat tagagacgct gcatattgcg ggtcgcgatg
    14701 tgctgtgtaa tgtctggcag gaagaggtga cttccacgct accagaaaaa cagtggcaaa
    14761 acacattctg ggtcgattcg gctactggcc aggttcgtca aagtcggcaa atgttaggcg
    14821 caggggttat tcccgtagaa atgacgtttc ttaaacccgc accatgaata aattacagtc
    14881 gtatttcatt gccagcgtac tttacgtaat gacaccccat gcctttgcgc aaggaacggt
    14941 gactatttat ctgcctggcg aacaacagac actttccgtt ggccccgtgg agaatgttgc
    15001 ccagttagtg acacaaccgc aactgcgcga tcgcctctgg tggcctggtg ccctgctgac
    15061 cgactccgcg gtgaaagcta aagcgcttaa agactaccaa catgtcatgg cacagcttgc
    15121 ttcctgggaa gcagaagctg acgacgatgt ggcggcaacg attaaatccg tgcgccagca
    15181 gttactcaat ctcaatatta ctggtcgctt accggtcaaa ctggaccctg attttgtacg
    15241 agtggatgag aacagcaatc ccccgctggt cgggaactat acgctctata ccgtacagcg
    15301 cccggtgact attactcttc tgggtgcggt ttctggggct gggcaattgc cctggcaggc
    15361 aggccgtagc gttaccgatt atctgcaaga tcacccccgt cttgcaggcg cggacaagaa
    15421 taacgttatc gtgattacgc ctgagggtga gactgtcgtc gcccctgttg ccctgtggaa
    15481 taaaagacat gtggaaccac cgcctggcag ccagctttgg ttaggctttt ctgcgcatgt
    15541 actgcccgaa aaatatgctg atttaaatga ccagattgtg tctgttttaa cgcaacgtgt
    15601 tcctgactga tgaagaagaa ttcttatctt ttaagctgcc tggccattgc cgtctccagt
    15661 gcctgtcatg ctgaagtatt aacctacccg gatccgctgg gttcgtcgca atcggacttt
    15721 ggcggcacag gattgttgca gatgccaaat gcgcgcatcg caccggaagg tgaattcagc
    15781 gtcaactacc gggataacga tcaataccgt ttctactcca cctccgtggc gctgttccca
    15841 tggctggaag gcaccattcg ttatacggat gtgcgcacac gtaaatatag ccagtgggaa
    15901 gatttcagcg gcgatcagtc atacaaagac aaatcattcg attttaaact tcgcctgtgg
    15961 gaagaaggtt actggctacc gcaagtggcg tttggtaaac gtgatattgc tggtacgggt
    16021 ctgtttgacg gtgagtatct ggtggccagc aagcaagcgg ggccatttga tttcaccctc
    16081 gggatggcat ggggctacgc cggtaatgcg ggcaatatta ccaacccgtt ttgccgggtg
    16141 agcgataaat attgtcatcg cgcagagtct cacgatgcgg gcgatatcag ctttagcgat
    16201 atctttcgtg gcccggcttc catctttggc ggcattgagt atcaaacgcc gtggaatccc
    16261 ctgcgtctga aactcgaata cgacggcaac aattaccaga atgatttcgc tggcaaactg
    16321 cctcaggcaa gccatttcaa cgtcggtgca gtttatcgcg ctgccagctg ggcagacctc
    16381 aacctgagtt atgaacgcgg taacacgttg atgtttggtt tcactttacg gaccaatttc
    16441 aacgatctgc gccctgccct gcgcgatacg ccaaaaccgg catatcaacc tgcgcctgaa
    16501 tctgaaggat tgcagtacac cacggtagca aaccaactta ccgccctgaa gtataacgcg
    16561 ggctttgacg cgccagaaat tcagctacgc gataagacgc tgtatatgtc tggtcagcaa
    16621 tacaaatacc gtgactctcg tgaagcggtc gatcgtgcca accggattct ggtgaataac
    16681 ctgccgcaag gcgttgagaa gattagcgtg acgcaaaagc gcgagcatat ggcgatggtg
    16741 actaccgaaa ccgacgtagc cagcctgcgc aaacagctgg caggtacagc gcctggtcaa
    16801 tcagagccac tgcaacaaca acgtgttgaa gctgaagatc tttctgcctt tggtcggggc
    16861 taccgtattc gtgaagatcg ctttagctac tctttcaacc caacactttc acagtcgctg
    16921 ggcggcccgg aagatttcta tatgttccag ctggggctga tgtccagcgc ccgctactgg
    16981 tttaccgacc acctgctgct tgatggcggt attttcacca atatttacaa caactacgac
    17041 aagtttaagt cttcgctgtt gcccgcggac tctaccctgc cccgcgtgcg cacgcatatc
    17101 cgtgattacg ttcgcaatga cgtttatctc aacaacttgc aagcgaatta ctttgccgac
    17161 ttaggcaatg gtttctatgg ccaggtgtat ggcggttatc tggaaacgat gtatgccggt
    17221 gtcggttccg agctgcttta tcgcccgcta gatgccagct gggcgctggg tgtggacgtt
    17281 aactacgtga agcagcgtga ctgggacaac atgatgcgct tcaccgatta ttccacgcca
    17341 actggtttcg tgacggctta ctggaacccg ccgacgctca atggcgtact gatgaaactt
    17401 agcgttgggc aatatctggc aaaagataaa ggggcaacga tcgacgtcgc caaacgcttt
    17461 gacagcggcg tggcggtagg ggtatgggcg gcaatcagta acgtatctaa agatgactac
    17521 ggcgaaggcg gctttagtaa aggtttttat atctcgattc cgttcgactt gatgaccatt
    17581 ggacctaacc gcaaccgcgc ggtggtttcg tggacaccat tgacgcgtga tggtggacaa
    17641 atgctgtcac gcaaatacca gctctatcca atgacggcag agcgagaagt accggttgga
    17701 caataacctg acgtaattca taacattaca ttgactgata aatataaacc aatgaaaaag
    17761 aacattttta agttttcggt actgacattg gctgttctgt ctttgacagc atgtaccctt
    17821 gttcctggtc agaatttatc gaccagtaac aaggatgtca ttgaactacc tgacaatcag
    17881 tatgacctgg acaaaatggt caatatctat cccgtcacgc caggattgat tgatcagttg
    17941 cgcgccaaac cgattatgtc gcaggctaac ccggagctgg agcagcaaat cgcgaactat
    18001 gaatatcgta tcggcattgg tgatgtgttg atggttacgg tctgggatca cccggaacta
    18061 accacccccg cagggcaata ccgtagcgcc agtgataccg gcaactgggt taacgccgat
    18121 ggtgcgattt tctaccccta tattggtcgc ctgaaagtgg ccgggaaaac acttacccag
    18181 gtacgcaacg agattacagc gcgtctggat tcagtcattg aaagcccgca agtcgatgtc
    18241 agcgtcgcgg cgttccgctc gcaaaaagcg tacgtcaccg gtgaagtcag caaatcgggt
    18301 cagcagccga ttaccaatat tcctctgacc atcatggatg cgattaacgc cgcgggcggt
    18361 ctgaccgcag atgcagactg gcgcaatgtg gtgctcaccc agaatggcgt aaagaccaaa
    18421 gtgaacctct atgcgctgat gcagcgtggc gacttgcgcc agaacaaact gctgcacccg
    18481 ggagatattt tatttattcc gcgcaacgat gatctgaaag tcttcgtgat gggtgaagtg
    18541 ggtaaacaga gcacgctgaa aatggaccgc agcggtatga ccctggcgga agcgctgggt
    18601 aacgctgagg gtatgaatca ggatgtcgct gatgcgacgg ggatttttgt tattcgtgca
    18661 acgcaaaaca agcagaatgg caaaatcgcc aatatttatc agctgaatgc gaaggacgca
    18721 tcggcaatga tactcggaac ggaattccag cttgaacctt acgacattgt ttatgtcact
    18781 accgcgcctc tggctcgttg gaaccgtgtg atttccctgc tggtaccgac gatttccggg
    18841 gtgcatgacc tgacagagac ttcacggtgg attcaaacat ggcccaacta aaatttaact
    18901 caatcctggt ggtttgtacc ggcaacattt gccgttcgcc cattggcgag cgcttgctgc
    18961 gtaaacgtct gccgggcgta aaagtcaaat cggcaggtgt tcatggtctg gtaaaacacc
    19021 ctgcagatgc gacagcggca gatgttgccg ctaatcatgg cgtatctctt gaaggacatg
    19081 ccggacgtaa gctcactgct gagatggcac gaaattacga tctgattctg gcaatggagt
    19141 cggaacatat tgctcaggtt accgcaatcg ctcccgaggt ccgcgggaag acaatgctgt
    19201 ttgggcaatg gctggaacag aaagagatcc cggatcccta tcgtaaaagt caggatgcat
    19261 ttgaacatgt ctacggaatg ttggagcgcg ccagtcagga atgggcgaag cgcctcagcc
    19321 ggtaatttga gtttataaaa tacgatgaca actaaaaata tgaatacgcc accaggcagc
    19381 actcaggaaa atgagatcga tctgcttcgt ctggtcggcg agttatggga tcaccgtaag
    19441 tttattatca gcgtgaccgc gttattcacg ctgatcgctg tcgcttactc gctgttaagc
    19501 acaccaattt atcaggcaga tactctggtc caggttgagc aaaaacaggg caacgccatt
    19561 ctcagcggcc tgagtgatat gatccctaac tcatcgcccg agtctgcacc ggagatccaa
    19621 ctgctgcaat cgcgcatgat tctcggtaaa accattgctg aactgaatct gcgcgacatg
    19681 gttgagcaga agtattttcc gattgtgggt cgcggctggg cgagattaac caaagagaaa
    19741 ccaggtgagc tggcgatcag ctggatgcat attccacaac tgaatggtca ggatcagcaa
    19801 ctgacactca cggttgggga aaacggccac tatacgctgg aaggtgaaga gttcaccgtc
    19861 aatggtatgg tcggccagcg tctggaaaaa gatggcgttg cgctgactat cgcggatatt
    19921 aaggccaaac caggaacaca gtttgtcctg agccaacgta ccgaactgga agcgattaac
    19981 gcgttgcagg aaacctttac cgttagcgaa cgcagtaaag aaagcgggat gctggaactt
    20041 accattactg gtgatgatcc ccagttgatt actcgtattc tgaacagcat cgctaacaac
    20101 tatttgcaac agaatatcgc tcgccaggcg gcgcaggatt cacaaagcct tgaattctta
    20161 cagcgccagt tgccagaagt gcgcagcgag ctggaccagg cggaagaaaa actcaacgtt
    20221 tatcgccagc agcgcgattc ggttgacctt aacctggaag ccaaagccgt tcttgagcag
    20281 attgtgaacg ttgataatca actcaatgag ctgaccttcc gcgaggcaga gatctcccag
    20341 ctgtataaga aagatcaccc aacttatcgt gcgctgctgg aaaaacgcca gacgctggag
    20401 caagaacgca aacgcctgaa taagcgggta tcggctatgc cttccaccca acaggaagtg
    20461 ttgcgtttaa gtcgtgacgt agaagcgggc cgtgcggtat atctgcaatt acttaaccgc
    20521 cagcaggagt tgagtatttc gaaatccagt gccattggta acgtgcggat tatcgacccg
    20581 gcagtcactc agccgcagcc agtgaaaccg aaaaaagcgt tgaatgtggt gcttggtttt
    20641 attcttggcc tgtttatttc ggtgggtgcc gtgctggcgc gtgcgatgtt gcgtcgtggt
    20701 gtagaagccc cggaacaact ggaagagcac ggcatcagcg tttatgccac catcccgatg
    20761 tccgagtggc tggataaacg tacccgtctg cgtaagaaaa atttattttc taatcagcag
    20821 cgccatcgta ctaaaaatat ccccttcctg gcggtggata acccggcgga ttctgctgtg
    20881 gaagccgtac gtgcgctacg aaccagtctg catttcgcta tgatggagac tgagaataac
    20941 attctgatga tcaccggtgc gacgccagac agtggtaaaa cgtttgtcag ttcaactctg
    21001 gcagcggtga tcgcccagtc cgatcaaaaa gtgttattta ttgatgccga cttacgccgt
    21061 ggttattcgc ataacctgtt taccgtgagt aatgaacatg gcttgtcgga atatctggca
    21121 ggtaaagatg agctcaacaa agtgatccag cattttggca aaggaggctt tgatgtgatt
    21181 actcgcggtc aggtgccacc taacccatct gaactgctga tgcgcgatcg gatgcgtcaa
    21241 ttactggaat gggcgaacga ccattacgat ctggtgattg ttgatacgcc gccgatgctg
    21301 gcggtgagcg atgctgcggt cgtggggcgc tctgtcggca ccagcctgct ggttgcgcgt
    21361 tttggcttga acaccgccaa agaggtgagc ttgtcaatgc agcgtctgga acaggctggc
    21421 gttaatatta aaggcgcaat cctcaatggt gtgattaaac gcgccagcac cgcttacagt
    21481 tacggctata actattacgg ttatagttac tccgagaaag agtaaaactg aaggtaaagt
    21541 gaccggatgc ggcgtgaacg ccttttccgg tctacgcatt agacagttct tcgtttgtca
    21601 tcagcctcag agcattcagg tagctgaatg ctctttttta tacattacaa actgcacgcc
    21661 ggtatgcgtg cttcattcac gatttgcgta aaaccggcca acgaacacat gccctgcgca
    21721 tttcgctctt cacatcctgc cagggtcagt ttcacctctc cgggcggcgt atttaatgac
    21781 agcggagttt tatcacgcat ctgctgtaaa gtctggaaga ccagcgaaac ctgaatccac
    21841 tggctgttat cgcttagccg acgccagcgt tcaaacacca gttcaccacc tggcggcgtg
    21901 ttatccggct gacctggaag cgtccagttg agctccagtg cgccgccgag atttgccaga
    21961 ttagtatcgt gtccggcgat aaacagcact gaagtgggta atgtcacacc atacgcctgt
    22021 ttttgcggtg gatggggagt caacgctatc atgatcaaat ccaacaacgg ggtggcgcgg
    22081 ctgcgggcaa cctctggcgt gcgttgtagt aaataaaatt gcgcgttatg caaacttagc
    22141 aacgtgttcc actggtgtga atcagtgatc cttccccacc ccggctccgg cattccctgt
    22201 gcctgttgca ggagaaatat ttccgtcagc attgatgcga ggcttaccgc accggttaat
    22261 gagacattgt cggcgctcac cttgagttcc gatggtaatg cctgcgttaa tgaacagctt
    22321 tcgtcctgtt tctcacggtt aaggcacaag tttgattgcg gaaaattaag cacccgttcc
    22381 agttcgcgaa acgccgtttg ccgatgcccg gtaaagtcag caattgaccc tcctgccctg
    22441 ctgaggatcg cgtcagtcac gttcgcgtta tccagttggc aaacgccagt ttttagagga
    22501 ttaaataacg gatcgggact ggacgtatct gcctgggtat gtacggttat tgcacagtca
    22561 ggtgccagcc cggcggcgaa ggcttcgcct gttttacggg tacgctcgtc gacatcagca
    22621 ataatcgcga cctgaccagg ctgcgggcag ccctttttcg tcagcaatcc gtcggctacc
    22681 agacgctggc gttggtaatg tccgagataa gcgattagct caccaccgcg cggtgtcagc
    22741 caacccagtt ttaccggcca gtttggccat gcgtctgggg tgacatcctg catcagttgc
    22801 gtggccttgg ttggggcacg cacaccatga cgactgacaa tcaccacact ttccagcttc
    22861 agctccggct ccggctcact ctgagcgaat gcagattgcg gggttaacgg aatcagaaga
    22921 gataaaaatg ggatcaagat cgctttcatc gatatgtttc cgcttttcat atccgacatt
    22981 attgattgcc tgatacgacg ctaatgcgcc gcatcagact tacgttcgtt aacttttcaa
    23041 tcgcgggtcc agccatacca acacaagggt ggagagcgaa cacatcaaca aaatgccgac
    23101 gaaccaaagt aaataccaca ttgttcctgc tccttagtac agctcgtttt cgttacggcg
    23161 gagagtttct gtcgtcatgc gcccccacat tttgtagtag ctccagagag tgtagagcaa
    23221 cacaatgggc aaaaatatca gcacgattac caacataata ctcagcgtca gctgactgga
    23281 agtgctgtcc cacaacgtca ggctggagat tggactcacg cttgacggca tgacaaaggg
    23341 gaacagcgtg atgcctgccg tgaaaatcac gccaaattgc atcaatgacg ccatcaaaaa
    23401 tccccaaccc gggcggccac gataaatcgc catcaccgtc agcaatgggc agaagaaccc
    23461 cagcagcggg aagatccaca aaacgggcga ctcgacaaaa ttattcatcc aggcaccagg
    23521 tagcactgcc accagtttca ttaacggatt ggaaggaccg ttagcatcct gggcgagcag
    23581 tacaaagcca tcaataccga cccacagcca gtaacccgcc agcaaaaagc acagcatcac
    23641 caacagtgca gcgcgtttag tcgacagctg tgaacgcaga tgaatcacac caacagtttt
    23701 cagttgtaac cagacgccac cttgcaaaat caccatccca aggctgagca atccgcacaa
    23761 taaagggaat ggcgtcagca gttgccagaa gctgccgaga tactccacgc gtaattgcgg
    23821 agtgaaggca aacggcacgc cgagcaacaa gttgccgaag gcgataccga agactaccgg
    23881 cggcaccaga ctgccgatga ccagaccggc atcccacatt ttacgccagc gtgcatcggc
    23941 gatttttccg cgataatcaa aggccagcgg gcggaagaac agcgagcaca gcaccaggat
    24001 catcgccaca taaaagccgg aaaacgccgc tgcatacact ctgggccagg cggcaaataa
    24061 tgccccacct gcgaggatca accagacctg attgccttcc cagtgcgcac caacgctatt
    24121 aatcactatc cggcgttcat catcatcacg cgccaccagc ggcagcaaac agccaatccc
    24181 catgtcaaat ccgtcggaga tcataaagac caccaggatc acgccaatca gcagccacca
    24241 gatgaagcgc aatgtttcat aatcaaacat gattttctcc tttacccctg ttgctgcgtc
    24301 ggttgttcac tctgcatcgc gctcggcccc agacgggcat atttctgcat caggtagact
    24361 tcggcgatta agaacagggt gtaaagccct acgatcatga tcagtgagaa agccagttgt
    24421 cctgtggtta aagcggagtg cgcggagtat gtcggtaaga tgtcctgtat cgcccacggc
    24481 tgacgaccaa actcggtcat aaaccacccg gcttcaatcg caatccacgg caacggcaaa
    24541 ctccagagcg ccattttcag cacccagcga tgctggtcga ttttgccacg cagcgtctgg
    24601 acaagcgcaa tcagcatcac cagtagcagc agggaaccac agcccaccat gatgcggaaa
    24661 ctccagaata ccggcgcaac ctgaggtatc gcgccacgca tcgccgcctg gtactgtgcg
    24721 gctgtgacat gattcatatc cggcgcatag cgggagagca acatgccgta gcccaggtcg
    24781 ccttccagct cccggaatgc ctgcaacaca tgcggctcac gattgccttg cgatatttcc
    24841 tgcattaaca gccaggccat acgtccgcgt tgcaagcgtg ggtaggtttc agccatcaaa
    24901 ttcttcagac ccggcacggg tttatctaat gagtgagtgg cgaggatccc taacagcacg
    24961 ggaattttga tggcaaaggc gttacgctct tgatcctgtt ccggccaggc aaccacatgg
    25021 aacggtgcag gagcaggttc cgtttgccac tccccttcca tcgccgccag ttttaccggt
    25081 tgtacttgcg cgacttcata cgcagaactg tctccgagtt gcagggtacc gataatcgcc
    25141 agagtaccga agacggaacc gatggcaaac gagcgtaatg cgacatcgcg ctcccgtccg
    25201 cgcagtaaat accaggcgct gatcgccata ataaacatgg ccccggtcac gtagcccgcc
    25261 attacggtgt gcacaaattt cacctggctg accgggttaa agaccagctc gctgaagctg
    25321 gtcatctcca tacgcagggt gtcgatatca aaatgcgcac cggtcgggta ttgcatccaa
    25381 ccgttggcat tcaatatcca caatgcagag agatttgaac cgaacgccac cagccacgtc
    25441 accagcaggt gctggtattt attcagccgc tgccagccga agaagaaaag cccgacaaag
    25501 gtggattcga ggaagaaggc cattaatgct tccatcgcca gcggtgcgcc aaaaatatcg
    25561 cccacatagt tggaatagaa tgaccagtta gtaccaaact gaaactccat ggtcaggccg
    25621 gtagccacgc caagagcaaa gttgataccg aagagcttac cccagaagcg cgtcatatcg
    25681 cggtagattg ttttgccggt gaccacgtaa atggtttcca tgatagccag caaaaaaatc
    25741 agccccaggg taaggggtac aaataaaaag tgatacagcg cggtcagagc aaactgccag
    25801 cgcgataaat caatgacatc ccacatgcgc actcctgtag gcgaagagag aaaaataacc
    25861 cgttaatggt agatctccct ctttatcctg aaactctacg accagaggta ttcgccgatt
    25921 gcgatattca taagcctgaa agcgatactt accgtctttt tacgtcggtg cagcttccgc
    25981 cagccactga catacctcac taagccgctg cgcagagtcg accaaatctt ctggcgctgc
    26041 cagcaccact tccggtattg ggcagatttc ataactttcc agtaacgggc cttttaaggt
    26101 gtccgtacag cgtaaatgcc agacatggcg taaccccgtg gcgttgatat agctctcgcc
    26161 atagccaatg gtacgaatct gaatatttcc cggcccacag agacgtgaga gaaaaagccg
    26221 atcagcctcg ctgatgggta attgcgtcag attaatgctg tgcggctgcg cgtcggggtt
    26281 acgtacatgc gccagtaact catgcgccaa cggtaggcca ttcatcaggc catcaatggg
    26341 cggcggtaac agcgaatctg tcggcaagag attttgcgtt gccgcctgcc acaacgccag
    26401 cggcgcgcag ccagcctcca gtttgtcctc cagcaacttt tcgccgcgac gtctgcgcac
    26461 ccgccataat ccgcagaaga tcgcctcctg tatttcactt tcgctgtcgt cagcctgctg
    26521 aatgcgcact gacacttcac cttccccgag aagcgtattg ataaagtgac gatcgtcggc
    26581 gttgagattt aacagatcga ctgccagcac ctcgcccaac ggcggctggc gttgacttag
    26641 ttgatgttgt aactcgttta acagcgcaat cacctgcggg ctgtgagcac attgctccag
    26701 ggccgccata ctcggttcat cattcacctg acaggtgatc ggcagtggat tcatgctgaa
    26761 actgtcatcg ttcggttgcg ttcttggccc cagcagatgg aaaaaagttt cgctcatgag
    26821 gcacgctcct gctgtggttc gacaagccca cgcatcaggt ttatcagttc cgcccacggg
    26881 tgaataccat tcagcacgcc gcgatagttt ccgccggtaa acactaatgt ggcgggaaag
    26941 cgaaagacgc caaagcgatc tccgatggct tcgctctgtt caaggtcagc aatcgccacc
    27001 tgccatgtgt agtcgggaaa ctcgcgcagt aattcgccaa tcattaccgg attatcgctg
    27061 acctctggcg tgcgtttcgg gtcactgctt aataacacca cgccgtctgg cgcttgcgta
    27121 agccagtcgt caagacggaa ttcactaacc ggcgtccagc cgcgcgccag cattcgttgc
    27181 cacaacgcat caaatggcgt gtcgttgctc atccctgctc ctccagtgtc atcctgtact
    27241 ggcgcaagcg aacgccttcg taattttcca tcgacagaca gtcataattg agacagcgcg
    27301 attcattagc cggttgcggc acaattcccc atgcagccag ctgcgccagc gccgcctgtt
    27361 ccgctgcggg cagttgctcc cgtgccagtt cgctcaggct gccgccgtag tcgtcgagca
    27421 ttgcgggttg cagaccgacg agggcaatat gtgctggcag atgtccgcgg atatctgcca
    27481 gcgccagcac ttcggagaaa ctgttctggt gcaggctcat tttcttcgcg ctgagataag
    27541 ccggaatacg ttctccggca taggttcgca gcgttcctgg ttccagcccg tagtcaatgg
    27601 catcgagaat caacagatgg ctggcgcttt cgacataccc cagcaagttc agtccctgag
    27661 taccgccatc gacaatctcc acagactcgg gccagtggta atgggcatac aaccgttccg
    27721 ccacccgcac gccgaaacct tcatcggccc acagcaggtt gcccagcccc attaccacca
    27781 cgcgttgctc gctcatgaac gctccttgtt acttattttg ccaaatttgt ggctacggta
    27841 gccgttgacc atggtggaga tcaccgtgtc gtcggacatg atgtcttcac gcagcgccat
    27901 atagacatga ccgatcacaa acgcgccaat cagccacatc cccagccgat gccagctgtg
    27961 aatgtccatt gagttgcctc ccgtccagta gaaaaattcc accacataac ggaacggcgc
    28021 aaaaatagcg tactggctgt gttcgctgta cagcgcaaag ccggtgatga tcataaagac
    28081 cgacatcagg aaatagccaa acatcgccgc ctgggcgatg gggttatggc ctatatcggc
    28141 actcggacgt tttgccagaa acagatacca gcggatttca taccacacgc cctgccacca
    28201 gcttttacgc catactggca cgataaacag ctcgcgggag tatcgattgc caacaaaagc
    28261 ccagtagatc cgcatcagca agaccacggt aaaaaccatc ccggcgctga agtgaattaa
    28321 cctgatgtag cccatataga acagatacgt cgcctcgccg ctgacggaag gtagcggttt
    28381 gccgataaag taaccggtga ccatcaacac cgccatgcat aacaccgtca gccagtgcca
    28441 gatacgcact ggcgcttcaa agacatagtg gctgacaacg ttgtcgcttt tctgttgcat
    28501 gatgattctc cttcgctgtt aacgcacctg cacggagatc agctcgctac cgtcgtcgcc
    28561 cagcacgtgt gttgaacagg cgaggcacgg atcaaagctg tgcagagtac gcaggatctc
    28621 cagcggttgc tcggggatcg ccattttggt gttcatcagc gccgcttcat aagcgccaat
    28681 ctgccctttg ggatcgcgcg ggctggcgtt ccaggtggtc ggcaccacgc actggtagag
    28741 atcaatcttg ccatcgcgaa tggcggccca gtggcctaac gccccgcgcg gcgcttcggt
    28801 aaaaccgaca ccacggcact ctgtcggcca ggttgtgggt tcccattttt ccgtggaggc
    28861 ggtggcaaga ttgccgtttt tcaggttagt catcagcttg ttgaagaaat actgcaactt
    28921 acctgcggcc cactgtgctt cgtgcgcgcg gcacaaaatg cggcccaacg ttgactggat
    28981 accggaaagc ggcaggttca gcgccgacat catgcgatcg accgactcaa cggtcgcagc
    29041 atcgcctttg tgataagcga ttaacgtgcg tgccagcggc cccacttcca tcgcgttacc
    29101 gcgccagcgc ggcgctttga tccacgagta gcgttcctgt tcattcagct gctgaatgtt
    29161 ggtatcgctg cctttgacat cgccggggtt gtaccacggg tcggtgatgc catcgaacgg
    29221 atgacgcccg acctgatcgt tgggataacg ataccaggcg tgatcgacaa actcctgcac
    29281 ctgctgcgga tcaaccaaat ccactggcag cacattgttg aagtcgccgt taatcaccgc
    29341 gccgccaggc atcagcagac ttttctcacc aaagtcgttg gcaatatccg ggaatgcgcc
    29401 gtagctgagg acacatttat cagaaagacc cgtgccgatt tcgctccacg gtttgttgaa
    29461 ctgaccgatg gctaaggcgt cggggatcat cacgttgtta atgaagtctg ccgtgcgggt
    29521 gataatcgac tgcaccaggt tcaggcgttc catattgacc gccccgaccg cgccgctttc
    29581 gtcaatgtta atggcgcaag gcatcccgcc gacaatccag tttggatgcg ggtttttacc
    29641 gccaaagacc gcgtggattt tgacaatttc acgctggaaa tcgagagctt cgagatagtg
    29701 ggcaaagccc atcaggttgg cttctggcgg caacttgtac tgcgggtgcc cccagtagcc
    29761 attgcggaag atccccaact gcccgccttc aacgaatttc ttcaggcggt tttgtacgtc
    29821 gaagaaatag ccaggggatg atttcggcca agaggagaga ctttgcgcca gttcggaggt
    29881 tttccgcggg tcggctttca gcgcatccaa cacatcgatc cagtccatcc cggcaagctg
    29941 atagaagtgc accagatgat cgtggcacca gagcgttgcc agcataatgt tgcggatgat
    30001 attggcgttg tccggcactt taataccgat ggcatcttcg atggcgtaaa ccgaagccag
    30061 agcgtgtacg ccagtacaga cgccgcagat acgttcaacg aacgcccacg catcgcgcgg
    30121 gtcgcgccct tgcaggatga tctccagccc gcgaaacatg gtgccgcagg agacggcatt
    30181 ggtgattaca ttctgatcgt taatattcac ttcgcagcgc atgtggcctt caatgcgcgt
    30241 aatcgggtcg accaccaggc ggcgtccggc attattgatg gtgtatccct gagtttcgta
    30301 ctgagtgctc atgcctgttt atcctcattg cctggctgat gttcggtttc tgtaggttgc
    30361 tgattatgac gtctgcgctg gtcaacggcg ctggcgactg cgtgcacacc aacagccgct
    30421 gccaccacac caagcgcggt taaaccgacg gtatcggcgg tggaatgagt acccatttgt
    30481 ggaatatcga ccacgcggct gtagaacgaa ccgcgatccc agaaaccatt ttccgcacag
    30541 cccaggcagc cgtgaccaga ctggattggg aaagaaacgc catcattcca gcgtgtggag
    30601 gaacaggcgt tataggtggt aggccctttg cagcccattt tgtacaggca ataacctttg
    30661 cgggcagcgt catcatccca actctggacg aactctccgg cgtcgaagtg agcgcggcga
    30721 tagcatttat cgtggatacg ctgaccataa aacatcagtg gacgacccat tctgtcgaca
    30781 tctggcaagc gatcaaaggt caccatgtaa gtaatgatgg cgctcatcac atccgggatc
    30841 ggcgggcagc caggtacttt gataatgggt ttgtcggtga tgactttgtc gatagacgtt
    30901 gcctgtgtcg gattaggtcg cgcggcctgc acgcagcccc aggacgcaca ggttccccag
    30961 gcgataatcg cgctggctcc ggcagcggca cgtttgagtt tctcaataaa cggtcgaccg
    31021 ctgctgatac agaacatccc ctgctcgccc agcggcggat taccttctac tgcgaggata
    31081 tatttgccat tgtattgcgt gatgatgtct tcaaagactt cttccgcctg ggttccggcg
    31141 gcagccatca aagtatcgtc gtaatcgagg gaaatcaggg aaaggatgac gtcctttgcc
    31201 agcgggtgag cggagcggat aaaagattcg gtacagcagg tgcattccag accgtggatc
    31261 cataccaccg gaatgcgcgg tttgttctcc agcgcccagg caatctttgg tgccattccc
    31321 gcgcctaatc ccagcgacgt ggcagccaga ctacaatatt tgagaaagct gcgccgggta
    31381 acgccctgac gccgcatggc ctggtaaaat gtttcctcgt tattcatatc gcacgtctct
    31441 tctccttgcg acaccggcag gacaccgggc gcttatggtc ggcaagcgac gtccctgtag
    31501 caaggcaaac catcgcttca cgtgagatgc tgaaaacgaa agctcatcct tctgcactgg
    31561 cgcacgtcgc cagaaagtat tgttaataaa gcgtagtgaa acttttgcac aaaacaatac
    31621 aaactgtgtg gatttatctt ttagcgataa aaatggacct atttttcttt ttgccgggcg
    31681 gtgggatgtt tagccagttg ctaaacgagt aaaagagaag gaatttatgg tgatgaaatg
    31741 atcaatctgc ttttatttca cgctatcaat gcacttttgg ctgtttttca ggcaaacaaa
    31801 cagattaggg gtttacacgc cgcatcggga tgtttatagt gcgcgtcatt ccggaagtgt
    31861 ggccgagcgg ttgaaggcac cggtcttgaa aaccggcgac ccgaaagggt tccagagttc
    31921 gaatctctgc gcttccgcca aataatcaag gggttaccaa atggtagccc ctttgctttt
    31981 ttggctcttg gtataaactt ggtataccaa ttggtatatt tttattcgga tgagattatc
    32041 ggtcatgagc ggaacctcag gttcctcgtc tgatgccgct cttgcaacac gttacgctgc
    32101 cgagtatttt tgtaaaacat ggactgctcc aggcctgaac caggcagaag gatacaaagc
    32161 catctcagat ctgtcgcatc actattttcg tgcagaaggc tctagccctc cccaatcttt
    32221 tttgtgacac cctgtttcac tgaacatgaa gacactcacc ttgtccctga tattcattag
    32281 catcatcctg attatttaac ttctctattt agggaagccc gtctgataca aagcttctat
    32341 tcctcgttaa atattctcca ttctggcgcc aagccctatc tatagctgat tgaaaacaac
    32401 tctaaaaacg taataatatc aaatagttta ttcctacagc cgatcatttt gagtaaaata
    32461 ttgacgagaa tttcacaaaa ggtagatttc atgtaaacgg ataaataaga ctatccaata
    32521 aa
//
LOCUS       DAZFDC010000042        32263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFDC010000042 DAZFDC010000000
VERSION     DAZFDC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.042"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(506..1177)
                     /gene="queE"
                     /locus_tag="ACHJ48_002070"
     CDS             complement(506..1177)
                     /gene="queE"
                     /locus_tag="ACHJ48_002070"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO4707630.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1332..1952
                     /locus_tag="ACHJ48_002071"
     CDS             1332..1952
                     /locus_tag="ACHJ48_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO4707631.1"
                     /translation="MKRDLNTLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLN
                     QYQRRADLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQ
                     QAQAQVTRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYN
                     VTIRKFPAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            1966..2640
                     /locus_tag="ACHJ48_002072"
     CDS             1966..2640
                     /locus_tag="ACHJ48_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4707632.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNYAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGKIIRNSIIPAFKKGDLAGGLQKGISDIESRLTGNNLA
                     TITPTDHPLPFSGWWALLVWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLAT"
     gene            2874..4022
                     /locus_tag="ACHJ48_002073"
     CDS             2874..4022
                     /locus_tag="ACHJ48_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4707633.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEEMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWLFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNMCRVFPMMLTPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSVRW"
     gene            4019..4933
                     /locus_tag="ACHJ48_002074"
     CDS             4019..4933
                     /locus_tag="ACHJ48_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO4707634.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKDRASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(4993..6291)
                     /gene="eno"
                     /locus_tag="ACHJ48_002075"
     CDS             complement(4993..6291)
                     /gene="eno"
                     /locus_tag="ACHJ48_002075"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO4707635.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(6379..8016)
                     /gene="pyrG"
                     /locus_tag="ACHJ48_002076"
     CDS             complement(6379..8016)
                     /gene="pyrG"
                     /locus_tag="ACHJ48_002076"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO4707636.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(8244..9035)
                     /gene="mazG"
                     /locus_tag="ACHJ48_002077"
     CDS             complement(8244..9035)
                     /gene="mazG"
                     /locus_tag="ACHJ48_002077"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO4707637.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(9106..9441)
                     /gene="mazF"
                     /locus_tag="ACHJ48_002078"
     CDS             complement(9106..9441)
                     /gene="mazF"
                     /locus_tag="ACHJ48_002078"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO4707638.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(9441..9689)
                     /gene="mazE"
                     /locus_tag="ACHJ48_002079"
     CDS             complement(9441..9689)
                     /gene="mazE"
                     /locus_tag="ACHJ48_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO4707639.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9767..12001)
                     /gene="relA"
                     /locus_tag="ACHJ48_002080"
     CDS             complement(9767..12001)
                     /gene="relA"
                     /locus_tag="ACHJ48_002080"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO4707640.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(12049..13350)
                     /gene="rlmD"
                     /locus_tag="ACHJ48_002081"
     CDS             complement(12049..13350)
                     /gene="rlmD"
                     /locus_tag="ACHJ48_002081"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO4707641.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLEVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNCLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            13407..16163
                     /gene="barA"
                     /locus_tag="ACHJ48_002082"
     CDS             13407..16163
                     /gene="barA"
                     /locus_tag="ACHJ48_002082"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO4707642.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(16396..17736)
                     /gene="gudD"
                     /locus_tag="ACHJ48_002083"
     CDS             complement(16396..17736)
                     /gene="gudD"
                     /locus_tag="ACHJ48_002083"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO4707643.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(17757..19097)
                     /gene="gudX"
                     /locus_tag="ACHJ48_002084"
                     /pseudo
     CDS             complement(17757..19097)
                     /gene="gudX"
                     /locus_tag="ACHJ48_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
     gene            complement(19099..20451)
                     /gene="gudP"
                     /locus_tag="ACHJ48_002085"
     CDS             complement(19099..20451)
                     /gene="gudP"
                     /locus_tag="ACHJ48_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO4707644.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIAISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(20886..21335)
                     /gene="yqcA"
                     /locus_tag="ACHJ48_002086"
     CDS             complement(20886..21335)
                     /gene="yqcA"
                     /locus_tag="ACHJ48_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO4707645.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(21353..22135)
                     /gene="truC"
                     /locus_tag="ACHJ48_002087"
     CDS             complement(21353..22135)
                     /gene="truC"
                     /locus_tag="ACHJ48_002087"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO4707646.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGERSS"
     gene            complement(22135..22464)
                     /gene="yqcC"
                     /locus_tag="ACHJ48_002088"
     CDS             complement(22135..22464)
                     /gene="yqcC"
                     /locus_tag="ACHJ48_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO4707647.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(23086..23631)
                     /gene="syd"
                     /locus_tag="ACHJ48_002089"
     CDS             complement(23086..23631)
                     /gene="syd"
                     /locus_tag="ACHJ48_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO4707648.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLAPNLAEFLNQLKPLL"
     gene            23699..24547
                     /gene="queF"
                     /locus_tag="ACHJ48_002090"
     CDS             23699..24547
                     /gene="queF"
                     /locus_tag="ACHJ48_002090"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO4707649.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFQGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            24659..26023
                     /gene="ppnN"
                     /locus_tag="ACHJ48_002091"
     CDS             24659..26023
                     /gene="ppnN"
                     /locus_tag="ACHJ48_002091"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO4707650.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            26580..27869
                     /gene="sdaC"
                     /locus_tag="ACHJ48_002092"
     CDS             26580..27869
                     /gene="sdaC"
                     /locus_tag="ACHJ48_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO4707651.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            27927..29294
                     /gene="sdaB"
                     /locus_tag="ACHJ48_002093"
     CDS             27927..29294
                     /gene="sdaB"
                     /locus_tag="ACHJ48_002093"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO4707652.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMCG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            29406..30161
                     /gene="xni"
                     /locus_tag="ACHJ48_002094"
     CDS             29406..30161
                     /gene="xni"
                     /locus_tag="ACHJ48_002094"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO4707653.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(30216..31364)
                     /gene="fucO"
                     /locus_tag="ACHJ48_002095"
     CDS             complement(30216..31364)
                     /gene="fucO"
                     /locus_tag="ACHJ48_002095"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO4707654.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(31392..32039)
                     /gene="fucA"
                     /locus_tag="ACHJ48_002096"
     CDS             complement(31392..32039)
                     /gene="fucA"
                     /locus_tag="ACHJ48_002096"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO4707655.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         7946 a         8432 c         7724 g         8161 t
ORIGIN      
        1 ggggttacgt ggatatgttg cttattacga gtactgctaa tataaaaact tgagaaagag
       61 ataacgggtt atatggtggt ttatccccac tggcgcgggg aactcctcga ctttaatttt
      121 ttcgctgccc gcttttgcgg tttatccccg ctggcgcggg gaactctcta aacataacct
      181 attattaatt aatgattttt taagccagtc acaatctacc aactttacag tatcacacaa
      241 acaacacatc cattatgtta aagagcactt aacccattga ttaaaaaggt aaatatttaa
      301 aataactcta tacaaactaa aatctaccaa actttaccgc aataattttc actccagcga
      361 aaaattaacg ccacagaatt tgtagagaat gataatgatt agtccgggat aggcaagatg
      421 gatgagtttt cagagacgac aaagaatata aatgagtaga agtctttaca ggttccaccc
      481 atttataacg cttataaatg tttaatcagg caatatttaa atatttatgg gtttgcatcg
      541 acaaacgcca attacgcgca atgcaggttt caatgcacaa acgtgtggca tcatcctttt
      601 ggctaatcgg ctgcagtgca atgactcgcg gtttatcatc ggtcagcgtc gccaaaagtt
      661 catccagtgc ttcaatatca cgtacgcgtc ccactggatg cttgatttcg ttggctcgct
      721 ccagtgcctg tgacaacact tcatagccgc cgcgcatgtt cagctttggc gatacggtaa
      781 cccaggtatt cggtgtacag cgtacttcat gagtaccact ggtttcgatc tggcagctaa
      841 aaccgttctt ttcgagcagg tcagtcagtg gcagcaaatc atgaatgcaa ggctcaccac
      901 ccgtaatcac cacatgccgc gcggtatatc cctggcgacc aatgacagcc agcaagtctt
      961 cactgctcgc cgccccccac ttatcactct ctttggtctt cgccagaatg ctgaaaaggg
     1021 agacttcccg atcctcaagc ttttcccagg tgtgtttggt gtcgcaccag gcacagccaa
     1081 ccgggcatcc ctgtaaacga ataaaaatgg cggggacgcc ggtaaagtaa ccctcacctt
     1141 gcagggtctg gaacatctcg ttaatcgggt actgcatagc attctctgtg aagtggataa
     1201 ttgttaatta ttgcagatcc tgccacaaca atcatgtctt attaacattc tgttacaggc
     1261 aggtttatgg caaaaagtgg attagatcat aaagaataaa atgaagcaga gtattatgtt
     1321 ttaaggaatt aattaaaagg gatttaaaca cgctcagaat attcatcgta attatattta
     1381 ttttcaattt atcaggctgt ggatataacg atattcaaac ctatgatgag caggttaacg
     1441 cctcctggtc tgaggtattg aatcaatatc aacgtcgcgc cgatcttatt cctaatcttg
     1501 tcgccagcat taaaggttat tccagccatg aacgagatgt actggaagcc gtaacattgg
     1561 ctcgtagcca ggctaatcgc gccagtagcg atcttcagca aactccggga gatgaacaaa
     1621 aattacaagc ctggcaacag gctcaagcgc aggtgactcg cactctcggt cagttaacga
     1681 taattagtga gcgttaccct gagttaaaat ctcaggaact ctatcaaaat ttaatggtac
     1741 agcttgaagg aagtgaaaat cggattgccg ttgcgcgagg aagatatatt aaagccattg
     1801 aacaatataa tgtcactatc cgcaaattcc ccgctgtatt aaccgcaaag gtcatggatt
     1861 acacaccaaa gaaaaattat ttgccggatg atgtcgccgc agtaagtaaa gccccaacaa
     1921 tagattttag ccagaatgct aatgctcatt aagcggggaa gttctatgcg aaattttatc
     1981 ttccttatgg ctttcttctg ttcatctgtg tttgccacac aaattccagt acctgaatca
     2041 cccaagtatg tgaatgactt aaccggcaca ttaacaaaca gcgaagtaaa caccctgacc
     2101 aaccagatta aagccctgac gcaaaaaaac tacgcacagt tggtcgtgtt agtggttgaa
     2161 acaacaggcg atgaaaccat cgagcagtac gcgacgcggg tattcgatag ctggaaaccg
     2221 ggtgataaag accgcgatga tggcgttcta ctgttagtcg cctggcagga tcataccgtg
     2281 cgtattgaaa tcggttacgg actggaaggt attattacgg atgcgcagtc cgggaaaatt
     2341 attcgcaaca gtattattcc tgcatttaaa aaaggcgacc tggctggtgg tttgcaaaag
     2401 ggaatcagcg atattgaaag tcgtctgacg gggaataatt tagcaacgat aacaccgacc
     2461 gatcaccctt tgccctttag cggttggtgg gcattgttag tctgggcgat agtactgacg
     2521 tttatttctg ccagaggata tatcaaaacg ctgggagtaa tttgcttcgc agctattgta
     2581 ctggcctttg ttttacctat agcaggcttc agcggaagct ggggagtact cgcaacatga
     2641 ctctgcttcg ccacaccgtt cctggctgtc gccatcgctt ttaccccctt cggcaaaaaa
     2701 gttcgggatt tgatgcgtga tgccaaccag ccatcacgcc atacaagaag caattcttcc
     2761 tcccactatt catcatcgtc atcctcttca ttcgacaatg acaattttag cggtggtggc
     2821 ggttcatctg gtggcggtgg cgcttccggg cgctggtaac aaaaggaatt gatatgcgta
     2881 tttttctctt acttctgatg ctatgcggtt ttcaggtatt cgctgaacga ttgccttcag
     2941 tgactgactt aaccgggaca ttaacgacag aagaacaaac tgctttaact caacaattac
     3001 agactctcga gcagcaaaac cacttccagg tggccgtgtt ggttacgccg accacagggg
     3061 gcaaaaacat taaacttgca gcctcgcaga gatacggggt atatcactgg aaacccgttg
     3121 gagagaagcg atatggcgaa ggtatcttaa ttctcgtcgt atggccggaa gggctggcgt
     3181 caatgaagat tggacatgga ctggaagaga tgttgccgcc ggaacaagct gcgcaaattg
     3241 ttcgttacca tatgcagcca gaattcgaaa aaaacaatct ctttgctgga ttaacgggcg
     3301 gaattgaaag catcgcacag tttacccata tcaaagcgaa ccttagccct ttggatgctt
     3361 tagccaatca tctgtttgct aatccgcaac gttcgctgcc atgtcttgcc tggacactgc
     3421 tgatgatcgt tgccatcgtt gtcctctggc tatttgccag ccgcccacgg cgtggcatat
     3481 ggatgatatc gatgattacc cctatggtct ggtttttctc ctttcaggac gatgtcatca
     3541 ttcggcgggt atcggtcgtt tgttttagtt tattgttagc aacaacctgc aagcagcgtc
     3601 tcgcggtagt ttttaatatg tgccgcgtat ttcctatgat gctgacccca aaaaataaaa
     3661 acgctaaagt gaaaaaacaa aagaagactc cgcaggttcg tgaatcagga cgaaattcat
     3721 tgtttgtcgt gatgctgata attattgtgg gatggtgcct ggtgttcgcc agtaataaag
     3781 atattgcatt catctcaaca attattgggt taattgatca ttttatcggc ccaataacca
     3841 ttgctggcat cgttctgctg atcattgtgg ccaaatttac tggcaatctt aagctggggt
     3901 caggagaaaa aagcaacagg aaaacgggga atcgtaatgc tcattctcgc tcttcgtcga
     3961 gaaattcttt ccgcggtggt ggcggttctt caggaggcgg cggttcatcc gtccgttggt
     4021 gatcgtaatg cgcttaatct ttattctatt cactttgtgg tgccttcctg ggcttgccca
     4081 gcaaattgct gtgccggaac tgcgccaaca ggtgaccgat attactggta cgttaagcac
     4141 ttctgaacaa caatcactga cacagcaact gcaggatatt acacataaaa cacgggcgca
     4201 ggtcgctgta ttagtagtgc cgtccaccgg agacgattcc attgaacaat atgctacaag
     4261 ggtttttgat agctggaaac tgggagataa gcagagaaac gatggtattt tacttctggt
     4321 tgcctgggag gatcatgctg ttcgtattga ggttggctac gggctggaag gggtggttac
     4381 cgacctgcaa gcggcgaaga ttatcaggga tatattgatc cctgccttca aaagtgatga
     4441 cctgatgggt ggattaacac tggcaagcga aaatatcggc gcgcttctgt tgaatggtga
     4501 attaccggaa gacagggggg attattacag tatcaatccc cctattccat tatcgcttgc
     4561 tgtaattata ttgctggcgg tactttcata ttttattgtt tttaccgatc cgtcgaattt
     4621 accctggatc actctcactg gcgccattta cgggatggta ttcctctatg ttgccgagcc
     4681 aggaccatgg accaatttaa tcgttgcctg cggtatgtta acgccttttg cgatcgtacc
     4741 actggttatt ttttggctta tcgtcaataa aaagctacgc gccaaataca aaaaactcag
     4801 taaagataga gcttcaagaa aaggttcttc ttcatcctct tccggtggcg gctcaagtgg
     4861 cggaggctca ggcggcggat ttagcggcgg gggcggttct tctggcggtg gcggcgcgtc
     4921 cggtcgctgg taactcaacc ataaaaaacg ccagcccgaa ggctggcatt tttaaatcag
     4981 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
     5041 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacgcggt cagaacggct
     5101 catagaacca gttttgatct ggcctgcagc agtaccaaca gccaggtcag cgatggtagc
     5161 gtcttcagtt tcgccagaac ggtgagagat aactgcagtg tagccagcgt ctttcgccat
     5221 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     5281 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     5341 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccatccca
     5401 gtcagattcg tccagaccgt cttcgatgga aacgatcggg tactgtttgg tcagttcttc
     5461 caggaagtga gtgaattctt cagaggtgaa tgctttgttg ccttcgccag ccagaacgta
     5521 tttaccgtct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     5581 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     5641 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     5701 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     5761 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     5821 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     5881 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     5941 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     6001 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     6061 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     6121 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     6181 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     6241 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     6301 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccagg gtacgcgttg
     6361 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     6421 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     6481 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     6541 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     6601 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtacagct ggcgaaccag
     6661 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     6721 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     6781 aaccgggtac ttacagtctg gcacaaattc cgtagagttg gcgttctcca tgttggcaac
     6841 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     6901 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     6961 accgccaggt acgaggattg catccagacc tttaaggatt tcaacgccgc gcgtttcaac
     7021 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     7081 cagtgcttca atcacggatt tgtaagcgtc cggcagttca atgtacttgc cgaccatacc
     7141 aatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     7201 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     7261 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     7321 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggaa cggcgcgatc
     7381 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     7441 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     7501 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatcg cttcgaggaa
     7561 cggcagggat tcgatatcac ctactgtacc gccgatttcg accagtacta cgtcatgacc
     7621 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgtac
     7681 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     7741 cgtggtgaag ttgttgcggc ggctcatttt ggtacggatg aaacgctcgt agtgccccag
     7801 gtccaggtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     7861 catagtacct ggatcgacgt tgatgtacgg atccagtttc atgatggtca cattgaggcc
     7921 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     7981 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     8041 aaagacgatg gaataaccag gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     8101 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     8161 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     8221 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     8281 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     8341 cgctccactt cgcgaaaacg acgctcgaat ttttcgttcg ctttttgcaa tgcgatttcc
     8401 gcttttgtcc ctaaatgacg agccagattg accgtggcaa acagcaggtc ccccatttcc
     8461 tcctccagtt tagcctggtc gacaacagcc tgccgcgctt cgtacatcac ctcgtcgatc
     8521 tcttcgtaga ctttatcgac taccggacca agcgtcgtcc aatcgaagcc aacgttggcg
     8581 caacgtttct ggattttttg cgcacgcatt aaagccggta aactacgagg aatatcgtcc
     8641 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     8701 agcacttcac tactgttttc ggcagaactg tcagcaaaaa catgcggatg gcgacgctct
     8761 aatttatcgc taatcgcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     8821 atttgggcgt aaaacaccac ctggaacagc agatcgccga gttcaccgcg cagatcgtca
     8881 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     8941 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccgggtc gcgcaggcgc
     9001 tgcataatag tgagcaaacg gtcgatttga ttcattagaa tgtcctgaaa attgcgggtc
     9061 cgtcaggtgg aaacctgtga ccagaataga agtgagttag taacactacc caatcagtac
     9121 gttaattttg gctttaatga gttgtaattc ctctggggca accgttcctt tcttcgttgc
     9181 tcctcttgcc cgccaggcga tactttttac ctgatcagct aacgctacgc catcacgttc
     9241 ctgaccggat aaaacaactt cgaacggata tccttttgat tgcgttgtac aaggaacaca
     9301 cagacacata cctgttttgt tgttgtacat gaacggactc aggacaacag ccggacgatg
     9361 tccggcttgc tcgctacctt ttgtcgggtc aaaatcaacc caaatcagat cgcccatatc
     9421 gggtacgtat cggcttacca ttaccagact tccttatctt tcggctctcc ccagtcgata
     9481 ttctcgtgga ggttttccgg cgtgatgtcg ttgaccagtt cagcaagcgt aaatacgggc
     9541 tctttacgca ctggctcaat aattaatttg ccatccacca ggtcaatctt cacttcatca
     9601 tcaatattca gattgagcgc ctgcattaac gtagccggga tccgcaccgc cggtgaattt
     9661 ccccaacgct ttacgctact gtggatcata accctttcct caaaccacta tcatatgtag
     9721 atacagtata tatcaatcta cattgtagat acgagcaaat ttcggcctaa ctcccgtgca
     9781 accgacgcgc gtcgataaca tccggcacct ggttgagttt acccagcacg cgccccagca
     9841 cttgcaggtt gtaaatctca atggtcatgt cgatggtcgc cagttgctgt ttggtgtcgc
     9901 tacggctggc aacgccaagc acgttcacct tctcgttggc gagaatggtc gtgatatcac
     9961 gtaacaaccc actacgatca ttagccacca cgcggaccac cagcgaatat ccggcggagt
    10021 agctctcacc ccataccgcg tcaacaatgc gttctggcgc atgggagcgc agttccgcca
    10081 gttgttcgca atcggcgcgg tgtactgaaa taccgcgtcc ctgggtaatg aagccgacaa
    10141 tctcatctcc aggaatcggc tggcagcagc gcgcgatgtg gtgcatcagg ttaccaacac
    10201 cttcaaccac tacacgaccg ttatctttac tgcggttttg cggcgtgtag cttttttgct
    10261 gaagctgttt cagcgcggcg gcgtcctgct cttcggcact cggcttatta aattgcgatt
    10321 gcaggaagtt caccatctga ttgagacgga tatccccgcc accaatcgcc gccagcaact
    10381 cgtcgacatc attgaagttg taacgcggca gcagatgttt ttctgcttct ttcaggctga
    10441 tccccagatg ttccagctcg tcgtcaagga tttgccgccc agccagaatg tttttgtcac
    10501 ggtcctgttt acggaaccag gcgtgaattt tcgaacgccc acggctggtt gtgacgtaac
    10561 cgaggtttgg gtttaaccag tcacggctgg ggttcggctg tttctgggtg ataatttcaa
    10621 tctggtcgcc catttgcagc tggtaggtga acggcacaat gcgcccgcca atttttgccc
    10681 cgatacagcg gtgtccgaca tcactgtgga tgtggtaagc gaagtccagc ggcgttgatc
    10741 ccgcaggcaa atcaacgaca tcacctttag gcgtaaagac gtacacccgg tcgtcaaaga
    10801 cctggctgcg tacttcgtcg agcatttcgc cggaatcagc catctcttcc tgccacgcaa
    10861 tcagtttacg cagccaggca atccggtctt catgtcccga acgtgcgccg ccagcagccg
    10921 cgccctcttt atatttccag tgcgcagcaa cacccaactc tgcatcttca tgcatctgtt
    10981 tggtgcggat ttggatctca acggtttttc cacccggccc cagaaccacg gtatgaatag
    11041 actgataacc gtttggtttc gggttagcga cgtaatcgtc aaactcatcc ggcaggtggc
    11101 gatagtgagt gtgcactatc cccagtgcgg cataacaatc ctgtaaacgc tcggcgacaa
    11161 tacgtaccgc acgcacatca aacagctcat cgaaggcgag gttctttttc tgcattttgc
    11221 gccagatgct gtagatgtgt ttcggacgac catacacttc agctttaacg ccttcagctt
    11281 tcatctcagc gcgcagatga ccgacaaact cttcgatata gtgttcgcgg tcgagacggc
    11341 gttcatgcaa cagttttgcg atgcgtttgt attcggtcgg gtggagataa cggaagcagt
    11401 aatcttccag ctcccatttc agttgcccaa tcccaagacg gtttgccaac ggcgcgtaga
    11461 tattggtgca ctcttttgcc gccagtacgc gttcatcttc cggcgcatct tttacttcac
    11521 gcagatgagc aatacgctcc gccagtttga tgaccacgca gcgaaaatca tcgaccatcg
    11581 ccaataacat ccggcgaacg ttatcgacct gttcggagga aacagaatca gtgtgcgtcg
    11641 ctttcagctg gcggatcgcc gccatatcac gcacgccgtg aataaggtta acgaccgact
    11701 taccgacgct ctcacgcagc acatcttcgc tgactacgtt ggcatcagcc agagggaaca
    11761 gcagcgccgc ccgcagcgtg tcaatgtcca tacttaatgt cgagaggatc tccaccatct
    11821 caacaccacg ccacaataac agactggcat ccggatgccc ctgcgtctgt tgcagacaat
    11881 acgcccaggt ttcggctaag cactcacacg acttctggct ggtaataccc agacttgcga
    11941 tccatttttc cggatcaaat tcaccagcct tattgatatg tgcacttctt accgcaacca
    12001 tcgtcctctc ctttagggac cagacctgcc gaaatcggca aatcgcaact atttaacgcg
    12061 cgagaaaagt accatcgatt ccagatgtcc cgtgtgtggg aacatatcca gcatcgccag
    12121 tcgcgcaatg gtatatcctg cttttaataa cgcttcgcta tcccgagcca gcgttgcagg
    12181 gttacaggat acataaacta tacgaatagg ttccagtttt ataatttgct gcataacacc
    12241 tgcggcacct gctcgcgccg ggtccagcaa cactttatcg aagccgtttt tcgcccacgg
    12301 ctgctttgtg acatcttctt caagattttc gtgataaaac gtcacattct gtaagcagtt
    12361 aagacgcgca ttctgctggc ctttttccac cagcgccgga acaccttcta cacccaccac
    12421 actggcagct tgtgtcgcca atggtagtgt aaagttgccc ataccgcaga acagatccag
    12481 tacgcgatct tcaggttcca cctccagcca ttccaacgca cgcgctacca ttttttggtt
    12541 cacacccgca ttaacctgaa taaaatcgcg cgggctaaaa gttaagcgca acccgtttga
    12601 gtcataccag ggcatctcac cagagacggt ttcgagtatc tcactatcgg gggcgagata
    12661 cagatccagg ccttcagaat gcgaaaagcg ttccagtttt tcgcgatctg ccgaacttag
    12721 cggtgcggta tggcgcaaaa tcatcagcgt gccgctggtt gcctgtacca gttcaacatg
    12781 accaagatgg cgcatagctt gtaagctgcc caggcatgcc ctgactttgg gcagcaatgc
    12841 ttcaagttgg ggcactaaaa tgggacattg tttaacgtcg acaatgtcac tggagcccgc
    12901 tttgcgaaac cccatctgaa gttgctgtgt tttcggtaag tagttcagac ttaaacgcgc
    12961 gcgacggcga tagccccagg gaacatcggc gatcacttca gagacttcgt gtttcattaa
    13021 tcgggcgagt gccgcacttt tgcttcgctg ctgtaaatcc acgctggcgt gttgttgctg
    13081 acagccgccg catacgccaa aatgaggaca gcgtggcgtt tcgcgttccg ggctatcgct
    13141 taaccggcgt acgactttag cgcgggcata ctgtttttta tcctcagtaa cagtaacttc
    13201 cgcgttttcc tgcggcaata atccggggat aaatagcgtt ttgccgttat gtcgcgccac
    13261 gccctgacca aaagagtcga ggtcgttgac tgaaacggtt atgatctgac gcgtcgtcgt
    13321 gcgtcgtttt gcagagtaga attgcgccat tggcgagact ttctcaattt aacagtgtga
    13381 ccttaattgt cccataacgg aactccatga ccaactacag cctgcgcgca cgcatgatga
    13441 ttctgatcct ggcaccgacc gtccttattg gtttattgct gagtatcttt ttcgtcgtgc
    13501 atcgctataa cgacttgcag cgtcaactgg aagatgccgg tgccagcatt attgagccgc
    13561 ttgcagtttc tactgaatat ggcatgagcc tgcaaaatcg cgaatctatc ggtcagttaa
    13621 taagcgtact gcatcgtcgc cattccgata ttgttcgcgc gatttcggtt tatgatgaaa
    13681 ataaccgact ctttgtcacc tccaattttc atcttgatcc ctcatcaatg cagctcggca
    13741 gcaacgtgcc gtttcctcgc cagctcactg tcactcgtga cggcgatatt atgatcctcc
    13801 gcacgccgat tatttctgag agttactccc ccgacgaatc gcccagtagc gatgccaaaa
    13861 atagtcaaaa tatgttggga tatattgcgc ttgagctgga tcttaaatcg gttcgcttgc
    13921 agcaatataa agagatcttt atttccagcg tgatgatgct gttttgtatc ggtattgcgc
    13981 ttatttttgg ctggcgctta atgcgcgatg taaccggtcc gattcgcaac atggtgaata
    14041 ctgtcgaccg catccgtcgc gggcaactcg acagccgagt ggaaggattt atgctcggcg
    14101 agctggatat gctgaaaaac ggtatcaact cgatggcaat gtcgctggct gcttatcacg
    14161 aagagatgca gcacaatatc gaccaggcga cgtccgatct gcgtgaaacg ctggagcaga
    14221 tggaaattca gaacgttgag ttggatctgg cgaaaaagcg cgcccaggaa gcggcgcgta
    14281 ttaaatccga gtttctggca aatatgtcac acgagctgcg tacaccactg aatggtgtta
    14341 ttggttttac ccgcctgacg ctgaaaacag aattaacacc aacgcagcgc gatcacctga
    14401 atacgattga acgttcggca aataatttgc tggcaattat taatgatgtt ctcgacttct
    14461 cgaaactgga agcaggtaag ctgattctgg aaagtattcc attcccacta cgcagcacgc
    14521 tggatgaagt cgttactctg ctggcacatt cttctcacga taaagggttg gaactgacgc
    14581 tcaatattaa aagcgacgtg cctgataacg tgatcggcga cccactgcga ttacagcaaa
    14641 tcatcactaa cctggtgggg aatgcaatta aattcaccga gaatggcaac attgatattc
    14701 tggtagaaaa acgtgcgctg agtaatacca aagtgcagat tgaagtgcag attcgggata
    14761 ccggcattgg tattcctgaa cgcgatcaat cgcgcttatt ccaggccttc cgacaggctg
    14821 atgccagtat ttcccgccgt catggtggca ccgggctggg gctggtgatt acacaaaaac
    14881 tggttaatga aatgggcggc gatatttcgt tccatagcca gccgaatcgc ggttcaactt
    14941 tctggttcca cattaatctc gatctgaacc cgaacattat tattgaaggg ccatccaccc
    15001 agtgcctcgc cggtaaacgc ctggcctatg tcgaaccaaa ctccgcagca gcgcaatgca
    15061 cgctggatat tttaagtgaa acgccgctgg aagtggttta tagcccaacg ttctccgcgc
    15121 tgcctcccgc gcattacgac atgatgttgt taggcatcgc ggtgaccttc cgcgagccgc
    15181 taacaatgca acatgagcga ttagcgaaag cggtatcgat gaccgatttc ctgatgctgg
    15241 cacttccttg ccatgcacaa gtcaatgctg aaaaactcaa gcaagatggt atcggcgcgt
    15301 gtctgctgaa accattaaca cctacgcgcc tgttgcctgc cctgacggaa ttttgtcatc
    15361 acaaacaaaa cacgcttttg cctgtaaccg atgaaagtaa gctggcaatg acagtcatgg
    15421 cggttgatga caaccccgct aacctgaaac ttatcggcgc attgctggaa gatatggtgc
    15481 aacatgtgga actttgcgat agcgggcatc aggcggttga acgggcgaaa cagatgccgt
    15541 tcgatttgat cttaatggat attcaaatgc ctgacatgga tggcattcgg gcctgcgagc
    15601 tcatccacca gctcccccat cagcaacaaa cgccggttat cgcggtaacg gcgcatgcaa
    15661 tggccgggca aaaagagaag ctgcttggcg cagggatgag cgattatctg gcgaaaccga
    15721 ttgaagaaga gcgattgcat aatttgttgt tgcgctacaa gcctggcagc ggtatttcgt
    15781 ctcgcgtcgt gacgcccgaa gtcaacgaaa ttgtggtgaa cccgaatgcg accctcgact
    15841 ggcaactggc actacgccag gcagcaggaa aaaccgattt agcgcgcgat atgctgcaaa
    15901 tgttactcga tttcctgcct gaagttcgca acaaagttga ggaacagctg gttggagaaa
    15961 acccggaagg cctggttgat ttgattcata aactgcatgg cagttgcggc tatagcggtg
    16021 tgccgcgtat gaagaatctc tgccaactta tcgaacaaca gctacgtagc ggtactaaag
    16081 aagaagattt ggaaccggag ctgctggaac tgttggacga gatggataat gtcgcgcgcg
    16141 aagccagcaa aattctcggg taacggacgt cttgtcgagt agcaatcggc tttttatgac
    16201 gctgttttca gattggtgac aaagtgcctg tgtttatgcc ggatgcggcg ttaacgcctt
    16261 atccggccta caaaaccaat taaattcaat aaattaaaaa gcatgtaggc ctgataagcg
    16321 tagcgcatca ggcaattttg tgtttggcat cagtctcaaa ccggctccag atagagccgg
    16381 ttttggtttt ttgtcttaac gcaccatgca cgggcgcttg ttatcgaacg tccagccagg
    16441 aatcagatac tgcattccca tcgcatcgtc acgcgcgcca agcccgtgtt tctgatacag
    16501 ctcatgggct ttcatcactt gatccatatc gatttctaca cccagccccg gtttttctgg
    16561 cacctgtacc agcccgcctt tgatctcaaa cggttctttg gtcaggcgct gattaccttc
    16621 ctgccagatc cagtgcgtat caatagcagt aattttaccc ggtgcagcgg cggcaacatg
    16681 ggtaaacatc gccagggaaa tatcgaagtg gttgttagag tgtgaacccc aggtcaggcc
    16741 aaattcatgg cacatttgcg ccacacgtac cgaaccttgc attgtccaga aatgcggatc
    16801 cgccagcggg atatcaacgg attgtaacga gagcgtatgg cccatctgcc gccagtcggt
    16861 ggcgatcata ttggttgcag tcggcaggcc agtcgcgcga cggaactctg ccatcacttc
    16921 acgcccggag aaaccttgct ccgcaccaca cggatcttct gcataagcca gcgaaccttt
    16981 caggtatttg ccgattttaa tcgcttcgtt cagcgaccag gcaccgttag gatcgagcgt
    17041 aatacgcgcc tgcgggaagc gttgcgccag tgccacaata gactcggcct cttcttcccc
    17101 ggccagtaca ccgcctttca gtttgaaatc gttgaagcca tatttttcat acgccgcttc
    17161 cgccaggcgc accaccgcat ccggcgtcat cgcttcttca tgacgcaggc gataccagtc
    17221 gcatgagtca tccggctggc tttgatacgg cagaggtgtg gctttgcgat taccgacgaa
    17281 gaacagataa ccgagcattt cgacttcgct acgctgttga ccatcgccca gcagggatgc
    17341 cacgttaacc cccagatgct gccccagcag atccagcatt gccgcttcta tcccggtaac
    17401 tacatgaata gtggtacgca aatcaaatgt ctgcaaaccg cgcccgccgg catcacgatc
    17461 ggcaaaagta ttacgcacca gcgtcagaac gtttttgtat tcacccagcg ttttacccac
    17521 taccagcgga attgcatctt ccagcgtttt acggattttc tcgccgccgg gaatttcccc
    17581 tacgccagtg tgaccagaat tatctttgat aatcacaata ttacgcgtga agaacggtgc
    17641 gtgtgcacca ctcagattca tcagcatact gtcatggccc gccaccggga tgacctgcat
    17701 ttcagtaaca acaggcgtcg taaattgaga actcatattt atgtcctttt tcagaatcaa
    17761 tgacggccga aaacgggacg tttacggtca aaggtccagc cggggatcag gtactgcatc
    17821 gggcctgcgt cgttacgcgc accgccaggc agacgtttat aggcctcatg tgccttttgt
    17881 acctgttccc agtccagttc cacgcccaga ccgggcgcat caggaacggc aatttttccg
    17941 tttttaatct ccagcggatt ttgggtcagg cgacaatcgc cctcctgcca aatccagtgg
    18001 gtatcgatag cggtaggatt acccggtgcc gccgcgccca catgggtaaa catcgccaga
    18061 gagatatcga aatggttgtt ggaatggcag ccccaggtca gcccccagtc gtcgcaaagc
    18121 tgcgccactc ggactgcacc ggaaagcgtc cagaagtgcg gatcggcaag tggaatatct
    18181 accgcattga gcatcaccgc atgacccatt tcgcgccagt tggtggcgat catattagtc
    18241 gcgacgggca agccggtcgc ccgccgaaac tccgccatca cttcacgtcc ggagaagccc
    18301 tgttctgcgc cgcatggatc ttcggcatag gtaagaacat cattcagccc tttgcacaga
    18361 gaaatggctt catcaagcag ccatgcaccg ttgggatcaa cggtaatccg cgcatccggg
    18421 aagcgtttct tcaatgcacg aacagtgtcg atttcttgct cgccaggtaa cacgccgccc
    18481 ttaagtttga aatctttaaa gccgtagcga tcctgtgagg cttccgccag acgcacaacg
    18541 gcttcgctgt tcatcgcttt ctgatggcgc aactgatacc actcatggtt gcccggcgta
    18601 ttttccagat aaggaagatc ggttttggtc cgatcaccga tataaaacag ataaccgagg
    18661 acggtaatag catcgcgttg cttgcctggc cctaacagtt cgcagaccgg aacattcagc
    18721 gccttaccta gcaggtcaag caaggcggct tccagcgccg ccacggcgtt aacgcgcaat
    18781 tcaaaggtcc aggccccttt accgaaggta tcaaaatcgg ctgcttgatt acctttatgc
    18841 acctgctgaa ccactttatt caggcgcgca acttcctagc ccagcaccat cggaatagca
    18901 tcgaccaatg tctgataaat cacctctccg cctggcgctt caccaatgcc ggtatgcccg
    18961 gcgttatcgg tgagtaccac aatattgcga gtgaaatatg cgttatgcgc cccaccaata
    19021 ttaagcaaca tgctgtcgta cccggcgacc ggaatgactt tcatatcagt aataacagga
    19081 ctggattgtg tcgtcatctt attgccccac gacaggtttc agctcgatac gcttgatatc
    19141 gcccaccagc accaggtagc tcagtaccgc gattaaggca tgaacaccaa cataaatcag
    19201 cgccccatta aacgagccag tcgtgccaac gatataacca attgcgattg gcgtgacgat
    19261 gccagaaatg ttaccgaaca tgttgaacag gccaccggaa agaccgctga tctcttttgg
    19321 cgcggtatct gccattactg cccaacccag cgccccaatg cccttaccga agaaggccag
    19381 cgccataaag ccgatgatca tccactcaac gttgacgtag ttgcagaaca ccatcaccat
    19441 cgacagcaac atgcccatta cgatcggtgt tttacgcgca atgttcagcg atcccgtgcg
    19501 gcgcatcagc cagtcggaaa taatcccacc cagcacacca ccgataaaac cacaaaccgc
    19561 cggaacggaa gccacaaagc ccgctttcag aatcgacatc ccacgcgcct gcaccagata
    19621 aaccggaaac caggtaatga aaaagtaagt cagggcgttg atacagtact gaccgatata
    19681 aacgccgatc atcatccgtg acccgagcag ttgtttgatc tgcccccact tcacgctgaa
    19741 cggcacttta actttggtgt tttgctgatc catattgatc agcgcgccac ccgcggcgat
    19801 gtactccagc tctttctgat ttacccccgg atgttgattt ggctcgtgga tgactttcaa
    19861 ccagataaag ctgatggcaa tgcccagacc gcccataaag aagaagacgt gtgaccagcc
    19921 cacttcatgc gtcagccagc ccataatcgg cgcgaagatc accgttgcga agtattgagc
    19981 ggagttaaaa atcgacaccg ccgttcccct ttcctgcgcc ggaaaccagg ccgcaacaat
    20041 gcgactgttg ccggggaaag atggcgcttc agcaagcccg accaggaagc gcagagtaaa
    20101 cagggcaacg ataatgccga atccactaaa gatatcgacg aagccttgca gcaaggtaaa
    20161 catcgaccag ataaagatcg accagaagta gacgcgtttt gaaccaaaac gatccagcaa
    20221 ccaaccacca gggatctgcc cgataacata agcccatgag aaggcagaga acacatagcc
    20281 cattcccacg ggatcaaggc cgatatcttt ggccatttcc gaaccggcaa tagaaagcgt
    20341 agcgcggtcg ccgtagttga aggatgtgac gataaacaac atcaccacta tccagtaacg
    20401 agcattcgtg cgtttttcca cactgctcgc agcctgactt aaagaactca ttgttgcact
    20461 cctgaaaatt cgcgttagcc acgctcactc tggactgcga catcgccagg aaatcagagg
    20521 tgacgtaggg tgttttttgc cgtttttata ggtcgttcgc cgaatacggc gcgtgtttat
    20581 atctggcaat agcagtataa aaagcgcgcc atagcggctc accgtgcaac aacacaacat
    20641 taatgcgttc aatgaggccc gattttggca ttagccctgg acggtggaat ccacttcacg
    20701 gaaatgaaaa caagaacaag aaaggaaggg ttaaaacgaa gaaataaaga agagtatgaa
    20761 atggatcgct tgactccagg caaacgccag taaaaatccg cgctatgaag cagtttttac
    20821 tggcatttgc ctgaaaagat tcgattcagc accgctaaaa cgacatttac cgctcgctga
    20881 acatatcagg acaacagcgt gccccactgt tcgacccacg gatttgattc cgtttccggt
    20941 tccgggttct cactggcgtc aatcaacagc atttcgccaa cccgctgagc gctctgttcc
    21001 tgcagcaagg catcgaactg tttgccgcca ttgcagaaat tcacataact actgtcgccg
    21061 agcgcaatca cgccataacg cagattcggc tggaagccca gactatcttt gattccctga
    21121 aagagcggca caatgctatc aggaaggtcg ccctgcccgg tcgtggacgt aaccaccaga
    21181 acatacttat cctgataggg cagccagtcg cttaattcag gatcttcaaa taccgttgct
    21241 ttgtggccct gtgcggtcag aattgcttct gcttcttccg ccactaacag cgaatttccg
    21301 tacatggtgc cgacaaaaat accaatttcc gccataccgt tttctccctg gattaggaac
    21361 ttctctctcc atcctgaccc gatggcgcac tgaactcaac cctttcattt tcaggaagca
    21421 gaccgcgcca gccaaattgt gataacgcct gcatccaggt gtcgtccagg cccgcgtgaa
    21481 tagtcagcgg ctcaccagta aaaggatgcg tcaatgacaa ctgactggca tgcagcatta
    21541 accgctgaag accaaaatgc tcagcaccgc tgcgattctg gcgtaaatcg ccatgtttgc
    21601 tatcgccaat aatcggatga cgcaaatggg caagatgtcg gcgaagctga tgtttgcgtc
    21661 cggttttcgg ctccagttcc accaggccgt agcgcgtggt cgggtaacgt ccggtcgcta
    21721 ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac tgctggctgc gggcctttat
    21781 cttcgcgggc aaatttatca gcgattttgt ccagttcttc caccagtgga taatccagca
    21841 ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta acgtttctgg atttggtgct
    21901 gttcaaactg ttgtgccagc agccgtccgg cctcgctgga taatcccatc aacaacacac
    21961 cagaagtggg tcggtccaga cgatgagcag taaaaacatg ctggcctatc tggtcacgca
    22021 cggtttgcat gaccactact ttctcgtcgc gatccagcca gctacggtga accagccagc
    22081 cggagggttt atttaccgca accagccatt catcctgata gagtatttcc agcattagct
    22141 cgcatcatcc gcaaaaagag catccagttt ttccaactca gccagaataa gcgcgcgttg
    22201 cggatggtcc gtcgccagcg ccatttcata atagggtgca acggcaaaag cgcccggtaa
    22261 cggctggttg ttatttaaca aatcgtgcat tcgcgggatc agcacccact gcaaccactc
    22321 cagtggttcc atggtgtcca taaagaacgg ttgggtacta ttaaattgat gcggctgggg
    22381 ttcatcgttt cgccagtgct gatgttcacg cagtaacgct tcaagcgcct gcaactggag
    22441 acgaacgcgg tcatgagtgg tcataaagca acctcaataa gaaaaactgc cgcgaaggat
    22501 agcaggaata aaaaaaggga gcactgtatt cacagtgctc ccggttcgtt tcgcagcatt
    22561 ccagctactt ttgttgctcc ctgctcatcc ttgacaactt ttcctctggc catgcggcca
    22621 atcgttcatc ctgaactatt gcttcctgct cacaccaccc cgatgtgata cttcatcctg
    22681 aagtgtccca ggccttcctg acccaccgaa tcatcctgac cggttctcat tctccatcct
    22741 ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct tcatccagaa gcctttccct
    22801 gaaacaccat cctggtgtgt ccttcagaag tgtcatcatc ctgatgttca cttcgttgtc
    22861 tgactccctg tcgacgaaga tagaatcgtc tttttcgcga actcttacaa ggcgcttaca
    22921 gacaatgcct taaggaaaat ttcatatgaa agttaggaat taacaagcaa catgttgttt
    22981 ttaaatgaaa ataacgattc ttatcttttt aagtattcgc ttatttcctg gcgatctcct
    23041 acagagtctg taagagatct ctcacaacca gatggggtat ttggattaca gaagaggctt
    23101 aagttgatta aggaattccg caagatttgg ggctaaatgc gtccgtttac gtgttcccag
    23161 cgtctcttta cacacttcac cggagagatt acacaccgag atgacctcta actcttcttc
    23221 cagcgtcgcg ataaatagcg tgggtggcag ttttagcctt ttctgagtca ccagatgacc
    23281 aatcagattt tcctgcaccc gacggaagtc gtcttcactc caggtttgca gcaatgtcag
    23341 tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac tgagtcgtat aaaaggtatg
    23401 aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc gcgtttacat tttgttcccc
    23461 ggtaaacggc tggggttgcc agtatacggc atcttcagta gttgaaataa tgcacggtga
    23521 aggaacgccg tacagttcct cacttaacgg ccagctttta tgctcttcat gccacgcatc
    23581 gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc aaatcgtcca ctaatttctc
    23641 tcttcactta aaccagatac acttgtcgtt tagtttatct ggtttatgac ggtgaaacat
    23701 gtcttcttat gcaaaccatc aggcacttgc gggcctgact cttggaaaat caaccgatta
    23761 ccgggatacc tatgacgcca gcctactgca aggcgttcca cgcagcctga atcgcgaccc
    23821 gctgggtctg aaagcggata acctgccttt tcaaggtacg gatatctgga cgctgtatga
    23881 actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct gtcggtcatg ttgaacttga
    23941 ttacaccagc gtaaatctga ttgagtcgaa gagttttaag ctctatctca acagttttaa
    24001 ccagacgcgt tttaataact gggatgaggt gcgccagacg ctggagcgcg acttaagcac
    24061 ttgcgctcag ggtgaggtta gcgtggcgtt atatcgtctt gatgaactgg aaggccagcc
    24121 gataggtcat ttcaatggca cttgcattga tgaccaggat atcactatcg ataactatga
    24181 attcactact gactatctgg agaatgccac cagtggtgaa aaagtagtgg aagagacgct
    24241 cgtcagccac ctgctgaaat caaactgcct gatcacccat caaccagatt ggggttcgat
    24301 ccaaattcag tatcgtggac gccaaattga cagagaaaaa ctgctgcgtt acctggtctc
    24361 attccgtcat cacaacgagt tccacgaaca gtgcgtggaa cgcatcttta atgacctgtt
    24421 acgcttctgc cagccagaga aattgagcgt ttacgcacgt tatacccgtc gtggcggtct
    24481 ggacattaac ccgtggcgca gtaatagcga ttttgtccca tcgaccacaa gactggttcg
    24541 gcaataaatt ttttctcaat tttgcgtgct ggattcacgc agaaggttgt gaaaggtcat
    24601 caggcagggc tattgtaatc aaagggaatg acgatattcg tcccataagg agtttttctt
    24661 gattacacat attagcccgc ttggctccat ggatatgttg tcgcagctgg aagtggatat
    24721 gcttaaacgc accgccagca gcgacctcta tcaactgttt cgcaactgtt cacttgccgt
    24781 actgaactcc ggtagtttga ccgataacag caaagaattg ctgtctcgtt ttgaaaattt
    24841 cgatattaac gtcttgcgcc gtgaacgcgg cgtaaagctg gaactgatta atcccccgga
    24901 agaggctttt gtcgatgggc gaattattcg cgctttgcag gccaacttgt tcgcggttct
    24961 gcgagacatt ctcttcgttt acgggcaaat ccataatacc gttcgttttc ccaacctgaa
    25021 tctcgacaac tccgtccaca tcactaacct ggtcttttcc atcttgcgta acgctcgcgc
    25081 gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg ggcggtcact caattaacga
    25141 aaatgagtat ttgtatgccc gtcgcgtcgg aaaccagctg ggcctgcgtg agctgaatat
    25201 ctgcaccggc tgtggtccgg gagcgatgga agcgccgatg aaaggtgctg cggtcggaca
    25261 cgcgcagcag cgttacaaag acagtcgttt tattggtatg acagagccgt cgattatcgc
    25321 cgctgaaccg cctaacccgc tggtcaacga attgatcatc atgccggata tcgaaaaacg
    25381 tctggaagcg tttgtccgta tcgctcacgg catcattatc ttccctggcg gtgtgggtac
    25441 ggcagaagag ttgctgtatt tgctgggaat tttaatgaac ccggccaaca aagatcaggt
    25501 tttaccattg atcctcaccg gcccgaaaga gagcgccgac tacttccgcg tactggacga
    25561 gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat taccgcataa tcattgatga
    25621 cgccgctgaa gtcgcccgtc agatgaaaaa atcgatgccg ctggtgaaag aaaatcgccg
    25681 tgatacaggc gatgcctaca gctttaactg gtcaatgcgc attgcgccag atttgcaaat
    25741 gccatttgag ccgtctcacg agaatatggc taatctgaag ctttacccgg atcaacctgt
    25801 tgaagtgctg gctgccgatc tgcgccgtgc gttctccggt attgtggcgg gtaacgtaaa
    25861 agaagtcggt attcgcgcca ttgaagagtt tggtccttac aaaatcaacg gcgataaaga
    25921 gattatgcgt cgtatggacg acctgctaca gggttttgtt gcccagcatc gtatgaagtt
    25981 gccaggctca gcctacatcc cttgctacga aatctgcacg taatctcccc tcccccggtg
    26041 acgtttgttg ccgggttttc tcgtttttgg tcacttactc atcaactcat ttcatttgtt
    26101 atatgaatgt ttcttaccac cctcacgcga caaatatcat cacagttaat atgtcataca
    26161 atttatattg caacgcaaac gtttccctat tttcataaac cgtattttat cgctatgaaa
    26221 aagaaattat cgccatgatt aactaaaagt attgattttt tcagttcaac ctacatatat
    26281 tgcgcgcccc ggaagaagtc agatgtcgtt taatgggcaa atattgccct taaattctct
    26341 tttacttttg atttacagag taaagcgttg ggataatcta tcttccaagt agattattgt
    26401 atttgggatc aagatcactg atagatacat aacttgtgtg tatctttccg ccctcaaatt
    26461 attacggcgg taaatgatta agccatcgcc gatagacaga tttcattttt acggtcaggc
    26521 accttcccgg gctgaactgg ctaaaagctg aattatttgc attcctccag gagaaataga
    26581 tggaaacgac tcaaaccagc acgattgcgt cgaaagactc tcgtagtgcc tggcgcaaga
    26641 cagacaccat gtggatgctg ggcctttacg gcacggcaat cggcgcgggc gtgctgttcc
    26701 tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat catcatggct atccttgcgt
    26761 tcccgatgac gttttttgct caccgcggcc tgactcgctt cgtactgtct ggtaaaaacc
    26821 cgggcgaaga cattaccgag gttgtagaag aacactttgg tattggcgca ggtaaactga
    26881 ttaccctgct ctacttcttc gctatctacc cgatcctgct ggtttatagc gtggcaatca
    26941 ccaatacagt tgaaagcttc atgtctcacc agctgggtat gacaccaccg ccgcgtgcga
    27001 ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt tcgctttggt gaacagatga
    27061 tcgttaaagc gatgagtatt ctggtattcc cgtttgttgg cgtactgatg ctgctggctc
    27121 tgtacctgat cccgcagtgg aacggcgcag cactggaaac gctgtctctg gacactgcat
    27181 ctgcaaccgg aaacggtctg tggatgaccc tgtggctggc aattccggta atggtgttct
    27241 cgttcaacca ctctccgatc atctcttctt tcgccgttgc gaagcgtgaa gagtacggcg
    27301 atatggcaga acagaaatgc tcgaagatcc tggcattcgc acacatcatg atggtgctga
    27361 ccgtaatgtt cttcgtcttc agctgcgtac tgagcctgac tccggcagac ctggctgcgg
    27421 ctaaagagca gaacatctcg attctgtctt acctggctaa ccactttaac gcaccggtta
    27481 tcgcgtggat ggctccgatt atcgcgatta tcgctatcac caaatccttc ctcggccact
    27541 acctgggcgc acgtgaaggc ttcaacggta tggtgattaa atctctgcgt ggtaaaggta
    27601 agtctatcga aatcaacaag ctgaaccgta tcactgcgct gttcatgctg gtaacgacct
    27661 ggattgttgc caccctgaac ccgagcatcc tgggtatgat tgaaaccctg ggcggcccaa
    27721 tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat tcagaaagta ccggcaatgc
    27781 gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt gatgggtctg attgcaatct
    27841 ccgcaatctt ctactctctg ttcagctaag tcctttcgcg ccgctttcgg gcggcgcttc
    27901 ctccgtttta acgcgatgta tttcctatga ttagcgtatt cgatattttc aaaatcggca
    27961 ttggcccttc cagttctcat accgttggac caatgaaagc gggtaaacaa tttaccgacg
    28021 atctgattgc ccgtaacctg cttaaagacg tgacccgcgt ggtggttgac gtgtacggct
    28081 cgctctctct gactggtaaa ggccaccaca ctgatatcgc cattattatg ggcctggcgg
    28141 gtaacctgcc ggataccgtg gatatcgatt ccatccctgg ttttattcag gatgtgaata
    28201 ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt ggagttcccg gttgatcagt
    28261 gcatgaactt ccatgccgac aacctttctc tgcatgaaaa cggtatgcgc attaccgcgc
    28321 tggcgggtga taaagtcgtt tacagtcaga cttactactc tattggcggt ggctttatcg
    28381 ttgatgaaga gcattttggc cagcaggata gcgcaccggt tgaagttcct tatccgtaca
    28441 gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg gctgtcactc tctggcctga
    28501 tgatgaaaaa cgagctggcg ctgcacagca aagaagagct ggaacagcac ctggcgaacg
    28561 tctgggaagt gatgtgtggc ggtattgagc gcggtatttc caccgaaggt gtgttacctg
    28621 gcaaactgcg tgttccacgc cgtgctgcgg cactacgccg gatgctggtc agccaggata
    28681 aaaccaccac tgacccgatg gcggttgttg actggatcaa catgtttgca ctggcagtga
    28741 acgaagagaa cgctgctggc ggtcgcgtgg tgactgcgcc gactaacggt gcgtgcggga
    28801 ttatcccggc agtgctggcg tactacgata agtttatccg cgaagtgaac gctaactcac
    28861 tggctcgtta cctgctggta gccagcgcca ttggttctct ttataagatg aacgcgtcga
    28921 tttctggtgc cgaagtgggt tgccagggtg aagttggtgt ggcgtgctca atggcggcgg
    28981 ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt gtgcatcgcg gcggaaatcg
    29041 ccatggagca caacctcggt ctgacgtgtg acccggtcgc cggacaggta caggtgccat
    29101 gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa cgccgcacgt atggcgctgc
    29161 gccgtaccag cgagccgcgc gtctgcctcg ataaagttat cgaaaccatg tacgaaacag
    29221 gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg cggcctggca atgaagatcg
    29281 ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg aggcctcttc ccgatttctc
    29341 atccagccat agcctgttcc agtatcgaat gttaccctta tcgcctgatc tttaaggggg
    29401 ttatcgtggc tgttcatttg cttattgtcg atgcactgaa tcttattcgt cgcattcatg
    29461 ccgttcaggg gtcgccctgt gtcgaaacct gccagcatgc gctcgatcag ctcattatgc
    29521 acagccagcc aacccacgcg gtcgccgttt ttgacgatga aaaccgcagt agcggctggc
    29581 gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc aatgccggaa gagttgcacg
    29641 acgagatgcc tgcattacgc gccgcctttg agcaacgcgg cgtcccgtgc tggtcaacca
    29701 gcggcaacga agccgatgac ttagccgcca cgctggcggt caaagtgaca caggccgggc
    29761 atcaggcaac gattgtttcg acagataaag gctactgtca gttactttca ccgacattac
    29821 gtattcgtga ttacttccag aaacgttggc tggatgcgcc atttatcgat aaagaatttg
    29881 gcgttcaacc gcagcagttg cccgattact ggggacttgc ggggatcagc agttcaaagg
    29941 taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca gctgctggtc gagtttcaga
    30001 gtctggaagg gatatatgag aatctggatg cggttgccga aaagtggcgc aaaaaattag
    30061 aaacccataa agagatggcg tttctgtgcc gcgatattgc ccgcttacaa accgatttgc
    30121 atatcgacgg caatttacag caattgcggt tggtacggta acggcgtgcc ggatacgccg
    30181 caaacgtcgc atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct
    30241 acaatatcct caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc
    30301 gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga
    30361 ataccgacat cacggttgag agcaaacacc gcttcaacag cggcattacg cgcctcttcc
    30421 aggctcatac cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg
    30481 gtaaagtcag cgttataacg catgacatgc ggtaacagga tggcgtttgc aacaccgtgt
    30541 ggagtgttat aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taaccccaca
    30601 ttcgagaagc ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta
    30661 tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt
    30721 gcatcggtta gcgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca
    30781 tcgacacccg tcgcagcttt cagcgctgga ggcataccat ccatcatgtc agcgtcaata
    30841 aacgccacct gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg
    30901 tcagtgatca cgtagttaat ggtcacttct gccgcagtgc ctgctgtggt ggggattgcc
    30961 agaatcggta cactgggttt attggtcggg gaaagtcctt ccaggctacg cacatcggca
    31021 aactccgggt tgttgctgat aatgccaatc gctttacaag tatcctgtgg agaaccacca
    31081 ccaatagcga tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg
    31141 acagtaattg ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca
    31201 tccatcttat cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg
    31261 atcagcgcct tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc
    31321 cgaccaaacc atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt
    31381 gctttacgaa attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg
    31441 gcaatctctt catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa
    31501 agttgcgcca gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca
    31561 caagcgataa gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca
    31621 acatgttcag aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta
    31681 ccgccagccg ccgcaatcat gtagtgaata gcggggatcg gtcggttaag aatggaaact
    31741 gctgtgcaat gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag
    31801 gccgccatat ggaaacgcca ctcgcttgag gggagctttc cttcctcatg tttaccgttg
    31861 ccatcaataa agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta
    31921 atcagcatcc catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc
    31981 aggcgggtca tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt
    32041 agctacctct ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt
    32101 agccgtttaa ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat
    32161 attttgtgac ctagttcaac taatcacagt aaataactgc aagttctctt tttataaccc
    32221 cattaaaaat gaccgctctt aaaaatattt atcaaaacgg tca
//
LOCUS       DAZFDC010000043        32214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFDC010000043 DAZFDC010000000
VERSION     DAZFDC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.043"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHJ48_002097"
     CDS             complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHJ48_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO4707656.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDERLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1957..2649
                     /locus_tag="ACHJ48_002098"
     CDS             1957..2649
                     /locus_tag="ACHJ48_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO4707657.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKSGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2848..3936
                     /gene="serC"
                     /locus_tag="ACHJ48_002099"
     CDS             2848..3936
                     /gene="serC"
                     /locus_tag="ACHJ48_002099"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO4707658.1"
                     /translation="MAQIFNFSSGPAMLPVEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4007..5290
                     /gene="aroA"
                     /locus_tag="ACHJ48_002100"
     CDS             4007..5290
                     /gene="aroA"
                     /locus_tag="ACHJ48_002100"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO4707659.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5459..6223
                     /gene="ycaL"
                     /locus_tag="ACHJ48_002101"
     CDS             5459..6223
                     /gene="ycaL"
                     /locus_tag="ACHJ48_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO4707660.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6396..7079
                     /gene="cmk"
                     /locus_tag="ACHJ48_002102"
     CDS             6396..7079
                     /gene="cmk"
                     /locus_tag="ACHJ48_002102"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO4707661.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7190..8863
                     /gene="rpsA"
                     /locus_tag="ACHJ48_002103"
     CDS             7190..8863
                     /gene="rpsA"
                     /locus_tag="ACHJ48_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO4707662.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9023..9307
                     /gene="ihfB"
                     /locus_tag="ACHJ48_002104"
     CDS             9023..9307
                     /gene="ihfB"
                     /locus_tag="ACHJ48_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO4707663.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9514..11778
                     /locus_tag="ACHJ48_002105"
     CDS             9514..11778
                     /locus_tag="ACHJ48_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO4707664.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILLAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GQRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVVILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILDQGLWFLADRSLALLFLGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGRNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11815..13563
                     /gene="msbA"
                     /locus_tag="ACHJ48_002106"
     CDS             11815..13563
                     /gene="msbA"
                     /locus_tag="ACHJ48_002106"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO4707665.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13560..14546
                     /gene="lpxK"
                     /locus_tag="ACHJ48_002107"
     CDS             13560..14546
                     /gene="lpxK"
                     /locus_tag="ACHJ48_002107"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO4707666.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14583..15815
                     /gene="ycaQ"
                     /locus_tag="ACHJ48_002108"
     CDS             14583..15815
                     /gene="ycaQ"
                     /locus_tag="ACHJ48_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO4707667.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEYPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     QGIFREQWLADYYRLKRPALAAWREARAEQRQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15867..16049
                     /gene="ycaR"
                     /locus_tag="ACHJ48_002109"
     CDS             15867..16049
                     /gene="ycaR"
                     /locus_tag="ACHJ48_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO4707668.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16046..16792
                     /gene="kdsB"
                     /locus_tag="ACHJ48_002110"
     CDS             16046..16792
                     /gene="kdsB"
                     /locus_tag="ACHJ48_002110"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO4707669.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16946..17839
                     /locus_tag="ACHJ48_002111"
     CDS             16946..17839
                     /locus_tag="ACHJ48_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO4707670.1"
                     /translation="MEQLRAELSHLLGEKLSRIEYVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17816..18595)
                     /gene="elyC"
                     /locus_tag="ACHJ48_002112"
     CDS             complement(17816..18595)
                     /gene="elyC"
                     /locus_tag="ACHJ48_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO4707671.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18731..19516
                     /gene="cmoM"
                     /locus_tag="ACHJ48_002113"
     CDS             18731..19516
                     /gene="cmoM"
                     /locus_tag="ACHJ48_002113"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO4707672.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPTQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19513..20835
                     /gene="mukF"
                     /locus_tag="ACHJ48_002114"
     CDS             19513..20835
                     /gene="mukF"
                     /locus_tag="ACHJ48_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO4707673.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALSYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20816..21520
                     /gene="mukE"
                     /locus_tag="ACHJ48_002115"
     CDS             20816..21520
                     /gene="mukE"
                     /locus_tag="ACHJ48_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO4707674.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDIDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21520..25980
                     /gene="mukB"
                     /locus_tag="ACHJ48_002116"
     CDS             21520..25980
                     /gene="mukB"
                     /locus_tag="ACHJ48_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO4707675.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26241..28088
                     /gene="ldtD"
                     /locus_tag="ACHJ48_002117"
     CDS             26241..28088
                     /gene="ldtD"
                     /locus_tag="ACHJ48_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4707676.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQQALDKGQLPTFVAGLAPQHPQYA
                     VMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28269..28817
                     /locus_tag="ACHJ48_002118"
     CDS             28269..28817
                     /locus_tag="ACHJ48_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO4707677.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28844..29491
                     /gene="gloC"
                     /locus_tag="ACHJ48_002119"
     CDS             28844..29491
                     /gene="gloC"
                     /locus_tag="ACHJ48_002119"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO4707678.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29542..30732)
                     /gene="aspC"
                     /locus_tag="ACHJ48_002120"
     CDS             complement(29542..30732)
                     /gene="aspC"
                     /locus_tag="ACHJ48_002120"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO4707679.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRIAADFLAKNTSVKRVWVSNPSWPNHKSVFNSADLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30917..32005)
                     /gene="ompF"
                     /locus_tag="ACHJ48_002121"
     CDS             complement(30917..32005)
                     /gene="ompF"
                     /locus_tag="ACHJ48_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO4707680.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7852 a         7842 c         8651 g         7869 t
ORIGIN      
        1 taaaaaaacg ccccgtaata taactcagac gaatcattaa gcctacatta cgtaggctat
       61 ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt aggccttcaa
      121 cagcgactga tgtgcagcaa acgcgtgcag atcgctatct actggttgca agccataaaa
      181 cgccgcttcg ccgctcattg ccgcactggc ggcttccacg gcatctgcgc cgtacatgcg
      241 aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgcgcca gtaataacaa
      301 cgtttgcagg cagcgataat agttggcgcg ttccgggctg aataccgatg agttaaactc
      361 catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca gcgccagcat
      421 ggcttttaat tcaccaatac gcagggtgta ccaaccgtta tccgacccgg tcgccagacc
      481 caacagctca cgcacgcggg taaagtcatc aaaaccttct tcatccagtt gctcgatgag
      541 gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg tttcacgtaa
      601 atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa tatcggacat
      661 gccaggcacg ataatacggc aagcataaac gcccagatgc tcgtaatcgg caatataaac
      721 ttctttatct tctttgttga agatagccat cagcgtagca aactcttctt ccgtggtgcc
      781 ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt cccaggagat
      841 taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga cttcttcatc
      901 atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt gcagcagctc
      961 ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag aggcaaagca
     1021 ggtgccgtta gcaggattaa acagtaccac gcaaatcacc ggatactggc cgccaaggga
     1081 accatcatat gcgaagatcg ggaaaccttc tgcttccagt gtttcgatcg cttcaaccac
     1141 cgctgggtaa cgcgccagca cgtctgccgg gatttccggc aagctgatgc tttcagcaat
     1201 aatgcggttt ttcacgtagc gttcgaaaac ttcggacaac ccctgaacgc gtgcttcgtt
     1261 acgggtattg ccagcagaca taccgttaga aacgtacagg ttaccaatga tattcatcgg
     1321 aatataaacg gtctgattat cagactgacg tgtaaacggc agaccgcaaa taccacgatc
     1381 ttcgttaccg gattgtaggt caatcagcat actgccggtc aattcattct ccggatcgta
     1441 aaatgcgcgc agacgttcat cgagcagccc ttctggcaca tcgtcatttt cggtcagtgg
     1501 gaaccatttt tcgttgggat aatgcacaaa tggaccgttg gcgatggttt cgcccagcca
     1561 gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccga gtgcagaagc
     1621 cagcgccgct ttcttggttg cgcctttacc gttggtaaaa cacagtgcgc actctttgtc
     1681 gcgaatatgt acagaccaga cgttaggcac gggattcagc caggaggcct cttcaatctg
     1741 aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca gtgcggcatc
     1801 tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga aagcgcgcaa
     1861 taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa ctgttgacta
     1921 tgcttttagc ggataaacca cgttaagagc ataaaaatga aggcgttcga tctccaccgt
     1981 atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg tagtctttat
     2041 acctttgtac tggtcttttt gttcctcaaa atgaccggaa gacgcggtgt gcggcagatg
     2101 tcgctgtttg aagttttaat cattctgacg ctgggatcag cggcgggaga tgtggcgttt
     2161 tatgatgatg tgccgatggt cccggtactt atcgtcttta ttactctggc gttgttatac
     2221 cgcctggtaa tgtggttgat ggcgcacagt gaaaaactgg aagatcttct ggaaggcaag
     2281 ccagttgtca ttattgaaga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     2341 acggaatttg agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta
     2401 cgtctggcga ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag
     2461 gtgaaatcgg gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg
     2521 gagtcggcgg actatgcctg tattcgttgt agtgaaatca ttcatatgaa agcgggggaa
     2581 aaacaattat gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg
     2641 gtgacctgac agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga
     2701 tgcaagccac atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat
     2761 aaaaccgata gccacaggaa taatgtatta cctgtggtcg caatcgattg accgcgggtt
     2821 aatagcaacg caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg
     2881 gcaatgctac cggtagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt
     2941 cttggtacgt cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag
     3001 gaagccgaga aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc
     3061 tgccatggcg gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa
     3121 accaccgcag attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa
     3181 aaatactgca cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt
     3241 aagccaatgc gtgaatggca actctctgat aatgctgctt atatgcatta ctgcccgaat
     3301 gaaaccatcg acggtatcgc catcgacgaa acgccagact tcggcaaaga tgtggtggtc
     3361 gccgccgact tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggtgta
     3421 atttacgctg gcgcgcagaa aaatatcggc ccggcaggcc tgacaatcgt catcgtccgt
     3481 gaagatttgc tgggcaaagc gaatgtcgcg tgtccgtcga ttctggatta ttccatcctc
     3541 aacgataacg gctccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg
     3601 gtctttaaat ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa
     3661 aaagcagaac tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg
     3721 aaagctaacc gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa
     3781 ttgttccttg aagagtcttt tgctgcaggc cttcatgcac tgaaaggtca ccgtgtggtc
     3841 ggcggaatgc gcgcgtctat ttataatgcc atgccgctgg aaggcgttaa agcgctgaca
     3901 gacttcatgg ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca
     3961 cagccagcct gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac
     4021 gttacaaccc atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc
     4081 taaccgcgct ttattgctgg cggcattagc acacggcaaa acagtattaa ccaatctgct
     4141 ggatagcgat gacgtgcgcc atatgctgaa tgcattaaca gcgttagggg taagctatac
     4201 gctttcagcc gatcgtacac gttgcgaaat tatcggtaac ggcggtccat tacacgcaga
     4261 aggtgccctg gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc
     4321 tctttgtctg ggtagcaatg atattgtgct gaccggtgag ccgcgtatga aagagcgccc
     4381 gattggtcat ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca
     4441 agaaaattat ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga
     4501 tggctccgtt tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga
     4561 agatacggtg attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact
     4621 caatctgatg aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt
     4681 aaaaggcggg cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc
     4741 ggcttcttac tttctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat
     4801 tggacgtaac agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc
     4861 gaccatttgc tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga
     4921 tatggatatg aaccatattc ctgatgcggc gatgaccatt gctacggcgg cgttatttgc
     4981 aaaaggcacc accacgctgc gcaatatcta taactggcgt gttaaagaaa ccgatcgcct
     5041 gtttgcgatg gcaacagaac tgcgtaaagt cggtgcggaa gtagaagagg ggcacgatta
     5101 cattcgtatc actccaccgg aaaaactgaa ttttgccgag atcgcgacat acaatgatca
     5161 ccggatggcg atgtgtttct cgctggtggc gttgtcagat acaccagtga cgattcttga
     5221 tcccaaatgt acggccaaaa catttccgga ttatttcgag cagctggcgc ggattagcca
     5281 ggcagcctga atgaataacg tccaataaat agccaaaact ttctttatca aaacgtcggc
     5341 acattgccgg cgtttttttt cggaccttgt gagtcatttt gattaatggt agcgtcgctt
     5401 gtcaatgtaa gttgttgata cataatgttt atatatgatt aatcaacgga tgattcacat
     5461 gaagaatact aaattactgc tggcgattgt gacctctgca gcattactga cggggtgtca
     5521 aaatacccac ggtattgata ccaatatggc tatcagctcc ggtttaaatg cctataaagc
     5581 agcaacgtta agcgatgccg atgcaaaagc gattgccaat cagggctgtg ccgaaatgga
     5641 cagcggcaat caagtcgcaa gtaaatccag caagtacggt aaacgtctgg caaaaatcgc
     5701 caaagcattg ggtaacaata ttaatggcac gccggtcaac tataaggttt atatgaccag
     5761 cgacgtcaac gcatgggcga tggcgaacgg ctgtgttcgt gtttacagtg gcctgatgga
     5821 catgatgaac gataacgaaa ttgaaggcgt tctgggccat gaactgggcc acgtcgcgtt
     5881 gggtcactcg ctggctgaaa tgaaagcttc ttatgcgatc gttgccgcac gcgatgccat
     5941 ttcagctacc agcggtgtgg cttcccagct ttcccgctca caattgggtg atatcgcaga
     6001 aggcgctatc aatgcgaaat actcccgcga taaagagtcc gaagcagatg atttctcctt
     6061 tgatctgttg aagaaacgtg gcatcagcac ccaggggctg gttggcagct ttgaaaaact
     6121 ggctagcctg gatggcggtc gcacccagtc catgtttgac tctcacccac catcaacaga
     6181 gcgtgcgcaa cacatccgtg atcgtatcgc ctctggtaag taaatcattg tcatctttcg
     6241 ggctggtctt ctgccagccc gctataattg cgcaataaat ccccatctga atacagacaa
     6301 aactggtttt tgcacacaac gttaacgatt tgtggcgtcg gcgcgtataa tgcgcgcggt
     6361 tatgttaacg gtacgcctgt tttaaggaga taaagatgac ggcaattgcc ccggttatta
     6421 ccattgatgg cccaagcggt gcagggaaag gcaccttgtg taaggctatg gcggaagcgt
     6481 tgcaatggca tctgctggat tcgggtgcaa tttatcgcgt actggcacta gcggcattac
     6541 atcaccatgt tgatgttgca tcggaagatg cgctggtgcc gctggcatcc catctggatg
     6601 tacgttttgt gtcaaccaat ggcaatctgg aagtgatcct cgaaggggaa gatgtcagcg
     6661 gcgaaattcg tactcaggaa gtggcgaatg cagcttcaca agtcgcggca ttcccacgcg
     6721 ttcgtgaagc attattgcgt cgccaacgcg cgtttcgcga attaccaggc ctgattgccg
     6781 atggccgcga catgggaacg gtggtattcc ctgatgcgcc agtgaaaatt ttccttgacg
     6841 cctcctcgga agaacgtgcg catcgccgca tgctacagtt gcaggagaag ggctttagtg
     6901 ttaactttga gcgccttttg gccgagatca aagaacgcga cgaccgcgat cgtaaccgag
     6961 cggtagcgcc actggttccg gcagccgatg ctttagtgtt ggattccacc accttaagca
     7021 ttgagcaagt gattgaaaaa gcgctacaat acgcgcgcca gaaattggct ctcgcataag
     7081 cgaccgaatt tgcagtaccc ccgttgcaat ggaatgacag cgggtatgtt aaacaacccc
     7141 atccggcatg gagccaggtg gacgttaaat ataaacctga agattaaaca tgactgaatc
     7201 ttttgctcaa ctctttgaag agtccttaaa agaaatcgaa acccgcccgg gttctatcgt
     7261 tcgtggcgtt gttgttgcta tcgacaaaga cgtagtactg gttgacgctg gtctgaaatc
     7321 tgagtccgcc atcccggctg agcagttcaa aaacgcccag ggcgagctgg aaatccaggt
     7381 aggtgacgaa gttgacgttg ctctggacgc agtagaagac ggcttcggtg aaactctgct
     7441 gtcccgtgag aaagctaaac gtcacgaagc ctggatcacg ctggaaaaag cttacgaaga
     7501 tgctgaaact gttaccggtg ttatcaacgg caaagttaag ggcggcttca ctgttgagct
     7561 gaacggtatt cgtgcgttcc tgccaggttc tctggtagac gttcgtccgg tgcgtgacac
     7621 tctgcacctg gaaggcaaag agcttgaatt caaagtaatc aagctggatc agaagcgcaa
     7681 caacgttgtt gtttctcgtc gtgccgttat cgaatccgaa aacagcgcag agcgcgatca
     7741 gctgctggaa aacctgcagg aaggcatgga agttaaaggt atcgttaaga acctcactga
     7801 ctacggtgca ttcgttgatc tgggcggcgt tgacggcctg ctgcacatca ctgacatggc
     7861 ctggaaacgc gttaagcatc cgagcgaaat cgtcaacgtg ggcgacgaaa tcactgttaa
     7921 agtgctgaag ttcgaccgcg aacgtacccg tgtatccctg ggcctgaaac agctgggcga
     7981 agatccgtgg gtagctatcg ctaaacgtta tccggaaggt accaaactga ctggtcgcgt
     8041 gaccaacctg accgactacg gctgcttcgt tgaaatcgaa gaaggcgttg aaggcctggt
     8101 acacgtttcc gaaatggatt ggaccaacaa aaacatccac ccgtccaaag ttgttaacgt
     8161 tggcgatgta gtggaagtta tggttctgga tatcgacgaa gaacgtcgtc gtatctccct
     8221 gggtctgaaa cagtgcaaag ctaacccgtg gcagcagttc gcggaaaccc acaacaaggg
     8281 cgaccgtgtt gaaggtaaaa tcaagtctat cactgacttc ggtatcttca tcggcctgga
     8341 cggcggcatc gacggcctgg ttcacctgtc tgacatctcc tggaacgttg caggcgaaga
     8401 agcagttcgt gaatacaaaa aaggcgacga aatcgctgca gttgttctgc aggttgacgc
     8461 agaacgtgaa cgtatctccc tgggcgttaa acagctcgca gaagatccgt tcaacaactg
     8521 ggttgctctg aacaagaaag gcgctatcgt aaccggtaaa gtaactgcag ttgacgctaa
     8581 aggcgcaacc gtagaactgg ctgacggcgt tgaaggttac ctgcgtgctt ctgaagcatc
     8641 ccgtgaccgc gttgaagacg ctaccctggt tctgagcgtt ggcgacgaag ttgaagctaa
     8701 attcaccggc gttgatcgta aaaaccgtgc aatcagcctg tctgttcgtg cgaaagacga
     8761 agctgacgag aaagatgcaa tcgcaactgt taacaaacag gaagatgcaa acttctccaa
     8821 caacgcaatg gctgaagctt tcaaagcagc taaaggcgag taattctctg actcttcggg
     8881 atttttattc tgaagtttgt tgagtttact tgacagattg caggtttcgt cctgtaatca
     8941 agcactaagg gcggctacgg ccgcccttaa tcaatgcagc aacagcagcc gcttaatttg
     9001 cctttaagga accggaggaa tcatgaccaa gtcagaattg atagaaagac ttgccaccca
     9061 gcaatcgcac attcccgcca agacggttga agatgcagta aaagagatgc tggagcatat
     9121 ggcctcgact cttgcgcagg gcgagcgtat tgaaatccgc ggtttcggca gtttctcttt
     9181 gcactaccgc gcaccacgta ccggacgtaa tccgaagact ggcgataaag tagaactgga
     9241 aggaaaatac gttcctcact ttaaacctgg taaagaactg cgcgatcgcg ccaatattta
     9301 cggttaagtt tttactcaaa cttgaacgag agaaaagcac ctgtcgggtg cttttttcat
     9361 ttatctaatc tggaactgga agctgcctcg cagagttttg agcagttttc accctttcgt
     9421 taaattcttc tgaatgtgcc tcggggaacg caaaattccc accagacaac cgctcaacaa
     9481 agttgcacac tttccataaa cagggagggg tgcatgaaaa taacgacagt cggcgtatgc
     9541 ataatttgcg gaatttttcc gttgctgatt ttgccccaat tgcctgggac attaaccctt
     9601 gcgtttctga ctctcttcgc ctgcgtactg gcatttatcc ctgttaaaac cgtccgttat
     9661 atcgcgctga cgttgctgtt tttcgtttgg ggcatattat cagcaaagca aattttgttg
     9721 gcaggagaaa ccttaactgg cgcgacgcag gatgcaattg ttgagatcac tgctactgac
     9781 ggcatgacca ctcattacgg tcaaattact tatctacaag gtcaacgtat attccctgcg
     9841 ccaggccttg tgctgtatgg cgaatatctt ccgcaagcgg tttgtgccgg acaactgtgg
     9901 tcaatgaaac tcaaagttcg tgccgttcat ggtcaactta atgatggcgg ctttgatagc
     9961 cagcgttatg ccattgccca gcatcagccg ctcaccggcc gctttctgca ggcaagtgtt
    10021 attgaaccga attgtagcct gcgtgcacag tatctggcgt cactacaaac aacgctgcaa
    10081 ccctatccgt ggaatgcggt tattcttggt ttaggtatgg gggaacggtt atccgtccct
    10141 aaagaaatca aaaatatcat gcgtgatact ggaacggcgc atttaatggc gatatcggga
    10201 ttgcacatcg cttttgcggc gttgctggct gccggactca ttcgcagtgg acaaattttt
    10261 ctgcctgggc gctggatcca ctggcaaatg ccattaattg gcggaatctg ctgtgctgct
    10321 ttttatgcct ggctgacggg aatgcaacct cctgcattgc gtaccgttgt ggcgcttgct
    10381 acgtggggta tgcttaagtt aagtgggcga cagtggagtg gctgggatgt atggatatgt
    10441 tgtctggcgg caattttgct gatggatcct gttgtcattc tctcgcaaag tttatggctc
    10501 tctgccgctg cggtcgcggc attgatattt tggtatcagt ggtttccctg tcctgagtgg
    10561 caactgccgc cggtattgcg tgcagttgtt tccctcatcc atctgcaact gggaatcaca
    10621 cttctgctta tgcccgtgca aatcgtcatt tttcatggca ttagtctgac ctcgtttatt
    10681 gcaaatctat tagcaattcc cttggtgaca tttatcacgg ttccgttgat cctcgccgcg
    10741 atggttgtgc atttaagcgg gccgttaatc ctggatcaag ggttatggtt tcttgccgac
    10801 cggtctttgg ctttactttt cttggggtta aagagtttgc cagaagggtg gatcaacatt
    10861 gctgaacgtt ggcaatggct atcattttcc ccatggttct tactggtggt atggcgatta
    10921 aacgcctggc gaacgttgcc agcaatgtgt gtggctgtag gcttgctgat gtgctggccg
    10981 ctgtggcaaa aacctcgacc tgacgagtgg caagtgtaca tgcttgatgt cgggcaaggg
    11041 ctggcaatgg tgatagccag aaacggcaaa gcgattctct atgacacggg actggcctgg
    11101 cctgaagggg atagtgggca acaactgatt atcccctggc tccactggca taatcttgaa
    11161 ccggaaggcg ttatcctgag tcatgaacat ctggatcacc ggggagggct ggactcaata
    11221 ttgcatacat ggccgatgtt atggatcaga agtccgttaa actgggaaca tcatcagccc
    11281 tgtgtgcgtg gcgaagcgtg gcaatggcaa ggattgcgtt tcagcgcgca ctggccttta
    11341 caaggtcgca acgataaagg aaataaccat tcctgtgtgg ttaaggttga tgacgggacg
    11401 aatagcattc ttctaaccgg tgatattgaa gccccagctg aacaaaagat gctaagccgt
    11461 tactggcagc aagtgcaggc aacattgctt caggtacctc accatggcag taatacctca
    11521 tcatcattgc cattaattca gcgagtgaat ggaaaagtgg cactcgcatc ggcatcgcgc
    11581 tataacgcat ggcgactgcc ctctaacaaa gttaagcatc gctatcaaca acaaggttat
    11641 acgtggcttg atactcctca tcaggggcaa gtaacggtcg atttttcagc gcaaggctgg
    11701 cggattagca gcctcaggga gcaaatttta cctcgttggt atcatcagtg gtttggcgtg
    11761 ccagtggata acgggtagaa tatgcggcta tttcaacaaa tgctggtttt ttgaatgcat
    11821 aacgacaaag atctctctac gtggcagaca ttccgccgac tgtggccaac cattgcgcct
    11881 tttaaagcgg gtctgatcgt ggcgggcgta gcgttaatcc tcaacgcagc cagcgatacc
    11941 ttcatgttat cgctccttaa gccacttctt gatgatggct ttggtaaaac agatcgctcc
    12001 gtgctggtgt ggatgccgct ggtggtgatc gggctgatga ttttgcgtgg tatcaccagc
    12061 tatgtctcca gctactgtat ctcctgggta tcaggaaagg tggtaatgac catgcgtcgc
    12121 cgcctgtttg gtcacatgat gggaatgccg gtttcattct ttgacaaaca gtcaacgggt
    12181 acgctgttgt cacgtatcac ctacgattcc gaacaggttg cttcttcctc ttccggcgca
    12241 ctgattactg ttgtgcgtga aggtgcgtcg atcatcggcc tgttcatcat gatgttctat
    12301 tacagttggc aattgtcgat cattttgatt gtgctggcac cgattgtttc gattgcgatt
    12361 cgcgtagtat cgaagcgttt tcgcaacatc agtaaaaaca tgcagaacac catggggcag
    12421 gtgaccacca gcgctgaaca aatgctgaaa ggccataaag aagtattgat tttcggtggt
    12481 caggaagtgg aaacgaaacg cttcgataaa gtcagcaacc gaatgcgtct tcaggggatg
    12541 aaaatggttt cagcctcttc catctctgat ccgatcattc agctgatcgc ctctttggcg
    12601 ctggcgtttg ttctgtatgc ggcgagcttc ccaagtgtca tggatagcct gactgccggt
    12661 acgattaccg ttgttttctc ttcaatgatt gcactgatgc gtccgctgaa atcgctgact
    12721 aacgttaacg cccagttcca gcgcggtatg gcggcttgtc agacactgtt taccattctg
    12781 gacagtgagc aggagaaaga cgaaggtaag cgcgtgatcg agcgtgcgac tggcgacgtg
    12841 gaattccgca acgtcacctt tacttatccg ggacgtgacg tacccgcatt gcgtaacatc
    12901 aacctgaaaa ttccggcagg gaaaacggtc gctctggttg gacgctctgg ttcaggtaaa
    12961 tcaaccatcg ccagcctgat cacgcgtttt tacgatattg atgaaggcga aatcctgatg
    13021 gatggtcacg atctgcgcga gtataccctg gcgtcgttgc gtaaccaggt tgctctggtg
    13081 tcgcagaatg tccatctgtt taacgatacg gttgctaaca acattgctta cgcacgaact
    13141 gaacagtaca gccgtgagca aattgaagaa gcggcgcgta tggcctacgc catggacttc
    13201 atcaataaga tggataacgg tctcgataca gtgattggtg aaaacggcgt actgctctct
    13261 ggcggtcagc gtcagcgtat tgctatcgct cgagccttgt tgcgtgatag cccgattctg
    13321 attctggatg aagctacctc ggctctggat accgaatccg aacgtgcgat tcaggcggca
    13381 ctggatgagt tgcagaaaaa ccgtacctct ctggtgattg cccaccgctt gtctaccatt
    13441 gaaaaggcag acgaaatcgt ggtcgtcgag gatggtgtca ttgtggaacg cggtacgcat
    13501 aacgatttgc ttgagcaccg cggcgtttac gcgcaacttc acaaaatgca gtttggccaa
    13561 tgatcgaaaa aatctggtct ggtgaatccc ctttgtggcg gctattgctg ccactctcct
    13621 ggttgtatgg cctggtgagt ggcgcgatcc gtctttgcta taaactaaaa ctgaagcgcg
    13681 cctggcgtgc ccccgtaccg gttgtcgtgg ttggtaatct caccgcaggc ggcaacggaa
    13741 aaaccccggt cgttgtctgg ctggtggaac agttgcaaca gcgcggtatt cgcgtggggg
    13801 tcgtatcgcg gggatatggt ggtaaggctg aatcttatcc gctgttattg tcggcagata
    13861 ccaccacagc acaggcgggt gatgaacctg tgttgattta tcaacgcact gatgcgcctg
    13921 ttgcggtttc tcccgttcgt tctgatgcgg taaaagccat tctggcgcaa caccctgatg
    13981 tgcagatcat cgtaaccgac gacggtttac agcattaccg tctggcgcgt gatgtggaaa
    14041 ttgtcgttat tgatggtgtg cgtcgctttg gcaatggctg gtggttgccg gcggggccaa
    14101 tgcgtgagcg agcggggcgc ttaaagtcag ttgatgcggt aatcgtcaac ggcggtgtcc
    14161 cccgcagcgg tgaaatcccc atgcatctgc tgccgggtca ggcggtgaat ttacgtaccg
    14221 gtacgcgttg tgacgttgct cagcttgaac atgtggtggc gatggcaggg attgggcatc
    14281 cgccgcgctt ttttgccacg ctgaagatgt gcggcgtaca accggaaaaa tgtgtaccgc
    14341 tggccgatca tcagtctttg aaccatgcgg atgtcagcgc gttggtaagc accgggcaaa
    14401 cgctggtaat gactgaaaaa gatgcggtga aatgccgggc ctttgcagaa gaaaattggt
    14461 ggtatttgcc cgttgacgca cagctttcag gtgatgaacc agcgaaactg cttgcgcaac
    14521 taacctcgct ggcttctggc aactagttac gccgcggcag cgttcgattg atggagtcat
    14581 gaatgtcgct gccgcacctc tcccttgctg atgcgcgtaa tcttcacctt gccgcgcaag
    14641 gcctgttaaa caaaccccgc cgtcgagcgt cgttggagga tattccggca acgatctccc
    14701 gcatgtcctt gctgcaaatc gataccatca atattgttgc ccgtagccca tatctggtgc
    14761 ttttcagtcg tctgggaaat tatcctgccc agtggctgga tgagtctctg gcgcgtggcg
    14821 aattaatgga atactgggcg catgaagcct gctttatgcc acgcagtgac tttcgtctta
    14881 ttcgccaccg catgctggca cctgaaaaaa tgggctggaa atacaaagac gcctggatgc
    14941 aggaacatga ggcggaaatt gcacagttaa ttcagcatat tcatgataag gggccggtac
    15001 gttcagccga ttttgagtat cctcgtaaag gtgcaagcgg ctggtgggaa tggaagccgc
    15061 ataaacggca tctggaaggt ttatttactg ccggaaaggt gatggtgatt gaacggcgca
    15121 acttccagcg cgtttatgat ttaacccacc gtgtcatgcc tgactgggat gatgagcgcg
    15181 atctcgtttc gcaaacagaa gcagaaatca tcatgctgga taacagtgcg cgtagccagg
    15241 gaatattccg cgaacagtgg ctggcagatt actatcggct gaaacgtccg gcactggcag
    15301 cgtggcgcga agcgagggct gaacagcggc aaatcattgc tgtgcatgtt gaaaaattgg
    15361 gcaatctttg gctgcatgct gatttgctgc cgctactcga gcgagcgctg gccggaaagc
    15421 tcactgcaac gcacagcgcg gtactttcgc cttttgatcc tgttgtctgg gatcgcaaac
    15481 gcgcagagca gctttttgat tttagctacc ggctggagtg ctatacccca gcgccgaaac
    15541 gccagtatgg ctattttgtt ctgccgttat tacatcgtgg gcaattagtt gggcgaatgg
    15601 atgccaaaat gcatcgccag acaggcatcc ttgaagttat ctctctgtgg ttacaggaag
    15661 gtattaaacc aacgacaacg ctgcaaaaag ggttacgtca ggcgattact gatttcgcta
    15721 actggcagca ggcaacgcgg gtgacattag gacactgccc gcaaggtctc tttacggatt
    15781 gccgcaccgg ctgggaaata gaccccgtcg cataaatgat tatgataaga tttatgcatt
    15841 cataacccga tccatctgga ggaactatgg atcatcgtct gcttgaaatc attgcctgcc
    15901 cggtttgcaa cggaaaactt tggtataacc aggaaaaaca agaacttatt tgcaaactgg
    15961 acaacctggc tttcccgctg cgcgatggca ttccagtgtt gctggaaacg gaagcccgcg
    16021 tgctgactgc tgatgagagt aaatcatgag ttttgtggtc attattcccg cgcgctacgc
    16081 gtccacgcgt ctgcccggta aaccattggt tgatattaac ggcaaaccca tgattgttca
    16141 tgttcttgaa cgcgcgcgcg aatcaggtgc cgagcgcatc atcgtggcaa ccgatcatga
    16201 ggatgtcgcc cgcgccgttg aagccgctgg cggtgaagta tgtatgacgc gcgccgatca
    16261 ccagtcaggg acagaacgct tggcggaagt tgtcgaaaaa tgcgcgttca gcgacgacac
    16321 ggtgatcgtt aatgtgcagg gtgatgaacc gatgatccct gcgacaatca ttcgtcaggt
    16381 tgctgataac ctcgctcagc gtcaggtggg tatggcgact ctggcggtgc caatccacaa
    16441 tgcggaagaa gcgtttaacc cgaatgcggt gaaagtggtt ctcgacgctg aagggtatgc
    16501 actgtacttc tcacgcgcta ccattccgtg ggatcgtgat cgttttgcaa aaggccttga
    16561 aaccgttggc gataacttcc tgcgtcatct tggtatttat ggctaccgtg caggctttat
    16621 ccgtcgttac gtcacctggc agccaagccc gttagaacac atcgaaatgt tagagcagct
    16681 tcgtgttttg tggtacggcg aaaaaatcca tgttgctgtc gcgcatgaag ttcctggcac
    16741 aggtgtggat acccctgaag atctcgagcg cgttcgcgct gaaatgcgct aatttcactt
    16801 cacgacactt cagccaattt tgggaggagt gtcgtaccgt tacgattttc ctcaattttt
    16861 cttttcaaca attgatctca ttcaggtgac atcttttata ttggcgctca ttatgaaagc
    16921 agtagctttt atgagggtaa tctgaatgga acagctgcgt gccgaattaa gccatttact
    16981 gggcgaaaaa ctcagtcgta ttgagtacgt caatgaaaaa gcggatacgg cgttgtgggc
    17041 tttgtatgac agccagggaa acccaatgcc gctaatggca agaagcttta gtacgcccgg
    17101 aaaagcccga caactggcat ggaaaaccac catgctggca agaagtggga ctgtccgtat
    17161 gccgactatt tatggtgtga tgacgcatga agaacacccc ggccctgatg tcctgctact
    17221 ggagcggatg cgtggtgttt cggtagaggc accagcccga acaccagaac gttgggaaca
    17281 gctcaaagac caaatcgttg aagccttact ggcctggcac cgtcaggaca gtcgcggttg
    17341 cgtcggcgcg gtcgacaata ctcaggaaaa tttctggccc tcatggtacc ggcaacatgt
    17401 tgaagtgcta tggaccacgc tcaatcagtt caataacacc ggtctgacga tgcaggataa
    17461 gcggatcctg tttcgcactc gcgaatgtct cccggcatta tttgaaggtt ttaacgacaa
    17521 ttgtgtgctg attcacggta acttctgttt acgcagcatg ttgaaagatt cgcgcagcga
    17581 tcagttactg gcgatggtcg ggccgggact aatgctttgg gcaccgcgag aatacgaact
    17641 gttccgacta atggataatt ctctggcgga agatttgctc tggagttacc tgcaacgcgc
    17701 gccagtggcg gagtcgttca tctggcggcg ttggttgtat gtgttatggg atgaagttgc
    17761 gcaactggtt aataccggac gatttagtcg gcgcaacttc gatctggcat caaaatcact
    17821 cttgccgtgg ctcgcctgac gaacctttta gccactgcca gatgcggcca agcgtttcat
    17881 agccaacacg atcgctatgc atcaaccaga ccggagaagg gattgcccgt tcccacgggt
    17941 taagcggcga gtcgatggcc aactgattcg ccggggctgg tagtggattt aacccttcct
    18001 gctgaaaaaa gatcatcgcc cgcggcagat gtgatgcgga ggtcaccagc aggaaagggg
    18061 cgtcaccaat cgcctgtttc actgccgcag cttcttcttc ggtatctttt ggcaaatcca
    18121 gggtgataat ttgctcgcgc ggtacgccca gcgattgcgc aactctggca cctacttccg
    18181 ctgtactcac cgtattggtt tttgctacgc ctcccgtgaa gatcagtttt gaacccggat
    18241 tttcgcgcca taaacggata ccctcattca ggcgtggcag actgttattg attaaattag
    18301 agctcggtgc ccactgcggg ttccaggtat aaccacctcc gagcaccacg atatagtcca
    18361 ctttttgcga attattccat gtcggatagg tgctttcgat gggacgcagc aggcggtcgg
    18421 ctaccggttg caaactcaat aacaagagtg ccagccaccc aatgctaata aaaatcttcc
    18481 cggttttttg aaaacggcta aaccacagta gcgccaggcc agcgccaata attaacaaca
    18541 tcagcggaag tggcagcaac atattgccaa tcactttttt cagtgtaaaa agcatccttt
    18601 ctggttcctt ttttaaccat atagcaaggg atcgccagcg atattacacc agacgggttc
    18661 attctcgccg tggctgtgac aaaatagcgt ctttatcaga aaacgtctta gccaaccggt
    18721 ggagaaagca atgcaggatc gcaattttga tgatattgcg gaaaagtttt cccgtaacat
    18781 ttacggcacc accaaagggc agcttcgaca ggctattctg tggcaggatc tcgatcgcgt
    18841 gctggcggaa atgggtccgc aaaaactgcg tgtgctggat gcgggcggtg gagaagggca
    18901 gaccgcaatc aaaatggccg agcgcgggca tcaggtcatt ttatgcgatc tttctgcgca
    18961 gatgatcgac cgcgcaaaac aggcggcaga agcaaaaggt gtgagcgaca acatgcaatt
    19021 tatacattgc gccgctcagg atgttgcttc gcatttggaa acgcccgttg atctgatatt
    19081 gttccatgcg gtgctcgagt gggtggctga tccccgcagc gtattgcaga ccctgtggtc
    19141 agtgttgcgt ccaggcggcg tgttgtcgtt aatgttctac aatgcgcatg gtttgttgat
    19201 gcataacatg gtcgccggga attttgatta cgtgcaggcg ggaatgccga aaaagaaaaa
    19261 acggacgctt tcgccagatt atccacgcga cccgacgcag gtttatctgt ggctggaaga
    19321 agctggttgg caaattatgg gtaagacagg cgttcgcgtg tttcatgatt atctgcgcga
    19381 gaagcaccag cagcgcgact gctatgaagc attacttgaa ttagaaacgc gttattgccg
    19441 tcaggaaccg tatattaccc tggggcgtta tattcatgtc accgcgcgca aaccgcagag
    19501 caaggataaa gtatgagtga attttcccag acagtccccg aactggttgc ctgggccaga
    19561 aaaaatgact tctccatttc gctgccggta gaccgactct cttttctgct ggcagttgcc
    19621 acgctgaacg gcgagcgtct ggatggtgag atgagtgaag gcgagctggt ggatgcattc
    19681 cgccatgtga gtgatgcgtt tgagcaaacc agcgaaacca tcggcgtgcg cgccaacaac
    19741 gcgatcaacg acatggtgcg tcaacgtctg ctgaaccgct ttaccagcga gcaggcagaa
    19801 gggaacgcaa tttaccgtct gacgccgctc ggcatcggca ttactgacta ttacatccgt
    19861 cagcgcgagt tttctacgct gcgcctttct atgcagttgt cgattgtggc gggtgagctc
    19921 aaacgcgcag cagatgccgc cgaagagggc ggtgatgaat ttcactggca ccgtaatgtc
    19981 tatgcgccac tgaaatattc ggtagcagaa attttcgaca gtatcgacct gacgcaacgt
    20041 ctgatggacg aacagcagca gcaggtgaag gacgatatcg cccagttgct gaacaaagac
    20101 tggcgggcgg cgatttccag ctgtgaattg ttgctttcgg aaacttccgg aacgctgcgt
    20161 gaattgcagg atacactgga agcggcaggc gacaaattgc aggctaatct gttgcgcatt
    20221 caggatgcga cgatgactca tgacgatctg catttcgtcg atcgtctggt gttcgatctg
    20281 cagagcaaac tcgaccgtat tatcagttgg ggccagcaat ccatcgattt gtggattggc
    20341 tacgaccgcc acgtacataa atttattcgt accgcgatcg atatggataa aaaccgcgtc
    20401 tttgctcagc ggttacgtca gtcggtacaa acctattttg atgagccgtg ggcgctaagt
    20461 tatgccaatg ccgatcgtct gctggatatg cgtgacgaag agatggcact gcgcgatgaa
    20521 gaagtgactg gggaacttcc gccagatttg gaatacgaag agtttaacga gatccgcgaa
    20581 cagctggcgg cgatcatcga agaacaactt gccgtgtaca aaaccagaca agtgccgctg
    20641 gatcttggtc tggtggtacg cgaatatctg tcacagtatc cgcgtgcacg tcactttgac
    20701 gttgcgcgca ttgttattga tcaggcggta cgtcttggcg tagcgcaagc agatttcacc
    20761 ggactgccag cgaaatggca gccgattaat gattacggag ccaaggtaca ggcgcatgtc
    20821 atcgacaaat attgaacaag tgatgccggt taagctggcg caggcgctgg cgaatccgtt
    20881 atttccggcg ctggacagcg ccttacgttc aggccgccat attggcctcg acgaactgga
    20941 taatcatgca ttcctgatgg attttcagga atatctggaa gagttttacg cgcgttataa
    21001 cgttgagctt attcgtgcac cggaagggtt cttctattta cgtccacgtt ccaccacgct
    21061 gatccctcgt tccgtcttgt cggaactgga tatgatggtc gggaaaatcc tctgttatct
    21121 ctatctcagc ccggaacggc tggcgaatga ggggattttc acccagcagg aactgtacga
    21181 cgaactgctc accctggccg atgaagcgaa actgctgaaa ctggtgaaca accgttcaac
    21241 cggttcagac attgaccgtc agaagttgca ggaaaaagta cgttcttcgc tcaaccgtct
    21301 gcgtcgttta ggtatggtgt ggtttatggg ccacgacagc agcaagttcc gcattaccga
    21361 atcggtgttc cgcttcggag ccgatgtgcg tgctggcgac gatccccgtg aagcacagcg
    21421 tcgcctgatt cgtgatggcg aagcaatgcc gattgaaaat catctgcaac tcaacgatga
    21481 aaccgaagag aatcagccag atagcggaga ggaagaataa tgattgaacg cggtaaattt
    21541 cgctcactga cgctgattaa ctggaacggc ttctttgccc gaacttttga ccttgacgag
    21601 ctggtcacga cgctttctgg cggtaacggg gcgggtaaat ccaccaccat ggcggcgttc
    21661 gttacggcgc tgatccccga cctgaccctg ctgcatttcc gtaacactac ggaagccggg
    21721 gccaccagcg gttcgcgcga taaaggtctg cacggtaagc tgaaagcggg tgtctgttat
    21781 tcgatgctcg acaccattaa ctcgcgccac cagcgcgtgg tggtcggtgt gcgtctgcaa
    21841 caggttgccg gacgcgatcg taaagtggat atcaaaccat ttgccattca gggactgccg
    21901 atgtcggtgc agccgacaca gctggtgacc gaaactctga acgaacgcca ggcgcgcgtg
    21961 ctgccgctta acgagctgaa agacaagctc gaggcgatgg aaggcgtgca gtttaaacag
    22021 ttcaactcca ttaccgatta ccactcgctg atgttcgatc tgggcatcat tgcgcgtcgt
    22081 ctgcgctccg catctgaccg tagcaaattc tatcgtctga tcgaagcttc gctgtatggc
    22141 gggatctcca gtgccattac ccgttctctg cgcgactacc tgttgccgga aaacagcggc
    22201 gtgcgtaaag cgttccagga catggaagcg gcgctgcgtg aaaaccgcat gacgctggaa
    22261 gcgattcgtg tcacccagtc ggaccgtgac ctgtttaagc atctgatcag cgaagctacc
    22321 aactacgtgg cggcggacta catgcgtcac gccaacgagc gccgtgtcca tctcgataag
    22381 gccctggagt ttcgtcgcga actacatact tcgcgtcagc aactggcggc ggagcagtac
    22441 aaacacgtcg atatggcgcg tgagctggca gagcacaacg gtgccgaagg cgatctggaa
    22501 gcggattatc aggcggccag cgatcacctg aacctggtgc aaaccgcact gcgtcagcag
    22561 gagaagatcg aacgctacga agcggatctc gatgagctgc agatccgtct ggaagagcaa
    22621 aacgaagtgg tggcagaagc catcgaacgc caggaagaga acgaggctcg cgcggaagct
    22681 gccgaactgg aagtggacga gctgaaaagc cagcttgctg actaccagca ggcgctggac
    22741 gtccagcaaa cgcgcgcgat ccagtacaac caggcgattg ctgcgcttaa tcgtgccaaa
    22801 gaactgtgcc atctgccgga cttaaccgcc gacagcgccg ccgaatggct ggaaaccttc
    22861 caggcgaaag agctggaagc gactgaaaaa atgctctctc ttgagcagaa aatgagcatg
    22921 gcgcaaaccg cgcacagcca gtttgagcag gcttatcagc tggtggtggc aatcaacggc
    22981 ccgctggcgc gtaacgaggc gtgggatgtc gctcgcgaac tattgcgcga aggggtcgat
    23041 cagcgtcacc tggcagagca ggttcagccg ttgcggatgc gcttaagcga actggaacag
    23101 cgtctgcgcg aacagcaaga agctgagcgt ctgctggcag atttctgcaa acgtcagggc
    23161 aagaattttg acattgacga gctggaagcc ctgcatcagg agctggaagc acgcattgcc
    23221 tctctttccg atagcgtgtc taacgcccgt gaagagcgga tggcactgcg ccaggagcag
    23281 gaacagctgc agtctcgcat tcagagtttg atgcagcgtg caccggtttg gctggcagcg
    23341 caaaacagtc tcaaccagtt gagcgaacag tgcggcgaag agttcacctc cagccaggac
    23401 gtcactgaat atctgcaaca gttgctggag cgtgagcgcg aggccattgt tgaacgcgat
    23461 gaagtaggcg cgcgcaaaaa cgccgtcgat gaagagatcg aacgtttaag ccagcctggc
    23521 ggctctgaag atcagcgtct gaacgcgcta gcggagcgtt ttggtggtgt gctgctgtca
    23581 gaaatttatg acgacgttag cctggaagat gcgccgtact tctcagcgct gtatggcccg
    23641 tcacgccacg ccatcgtggt gccagatctg tcacaggtaa ccgaacacct ggaaggtttg
    23701 accgattgcc cggaagatct ctatttgatc gaaggggatc cgcagtcatt cgatgacagc
    23761 gtgttcagcg ttgatgagct ggaaaaagcg gtagtggtga aaatcgccga tcgtcagtgg
    23821 cgttattcac gtttcccgga agtgccgctg tttggtcgtg ctgcgcgtga aagccgtatt
    23881 gaaagcctcc atgccgagcg tgaagtgctt tccgaacgct tcgccacgct ctcctttgat
    23941 gtacagaaaa cccagcgtct gcatcaggcg ttcagccgct ttatcggcag tcatctggcg
    24001 gtggcgtttg agtctgaccc ggaagcagaa atccgtcaac tgaacagccg tcgcgtcgaa
    24061 ctggagcggg cgttgagtaa tcatgaaaat gataaccagc agcagcgtat tcagtttgag
    24121 caggcgaaag aaggcgttac ggcgctgaac cgcattctgc cgcgtctcaa cctgttggct
    24181 gatgacagcc tggcggatcg cgtcgatgaa atccgcgaac gtctggatga agcccaggaa
    24241 gccgcgcgtt ttgttcagca gtttggcaat caactggcga aactggaacc gatcgtttcg
    24301 gtattgcaga gcgacccgga acagttcgaa cagttaaaag aagattacgc gtactctcag
    24361 cagatgcagc gcgatgcccg tcagcaggcg tttgccctga cggaagtggt gcagcgtcgt
    24421 gcgcacttta gctactctga ctcggcagaa atgcttagcg gtaacagcga tctcaacgaa
    24481 aaactgcgtg aacgtctgga acaggcggaa gcggagcgta cccgcgctcg cgaagcgttg
    24541 cgcggtcacg cagcgcagtt gagtcagtac aaccaggtgc tggcttcgct gaaaagttct
    24601 tacgacacca aaaaagagct actcaacgat ctgcaacgtg aattgcagga tatcggcgtg
    24661 cgtgctgata gcggggcaga agagcgggca cgtattcgcc gtgacgagct gcatgcacaa
    24721 ctgagcaata accgttcgcg ccgcaatcaa ctggaaaaag cgctcacctt ctgcgaagcg
    24781 gagatggata acctgacccg caaactgcgc aagctggagc gtgattactt tgagatgcgc
    24841 gagcaggtag tgaccgcgaa agcgggctgg tgtgcagtca tgcgcatggt gaaagataac
    24901 ggcgttgagc gccgcttaca ccgtcgtgag ctggcttatc tctccgctga tgatttgcgt
    24961 tccatgtcgg ataaggcgtt aggtgcgctg cgtctggcgg tggcggataa cgaacatctg
    25021 cgcgacgtgc tgcgcatgtc ggaagatccc aaacgtccgg agcgtaaaat tcagttcttc
    25081 gtggcggttt atcagcatct gcgtgaacgt attcgtcagg atattattcg taccgatgat
    25141 ccggtggaag ctatcgaaca gatggagatc gaacttagcc gtctgaccga agagttaacc
    25201 tcccgtgaac agaaactggc gatcagttcc cgcagcgtgg cgaacatcat tcgcaaaacc
    25261 attcagcgcg agcagaaccg tatccgtatg ctcaaccagg ggttgcagaa cgtatcgttt
    25321 ggtcaggtga acagcgtgcg cctcaacgtg aacgtgcgtg aaacgcacgc catgctgctg
    25381 gatgtgctct ctgaacagca tgagcagcat caggatctgt ttaacagcaa ccgtctgacc
    25441 ttctcggaag cgctggcgaa attgtatcag cgtcttaatc cgcagattga tatggggcag
    25501 cgcacgccgc agaccattgg cgaagaactg ctggattacc gcaactatct ggaaatggaa
    25561 gttgaggtta accgtggttc cgatggctgg ttgcgcgcag agtctggtgc attgtcgacc
    25621 ggtgaggcga ttggtaccgg tatgtcgatt ctggtgatgg tggtacaaag ctgggaagat
    25681 gaatctcgcc gcctgcgcgg taaagatatc tctccttgcc gcctgctgtt cctcgatgaa
    25741 gcagcgcgac tggatgctcg ttctatcgcc acgctgtttg aattgtgtga gcgtttgcaa
    25801 atgcagctca tcatcgcagc gccggaaaat atcagcccgg agaaaggcac cacctataaa
    25861 ctggtgcgta aagtcttcca gaataccgaa catgttcatg tcgtcggcct gcgaggattt
    25921 gcgccgcaac tccctgaaac gcttccagga agtgacgaag cgccttctca ggcgagttaa
    25981 aattaaggcg gcagcaatgc cgccttttct ttttccgaaa actccgtttc tgcactaaaa
    26041 aagtggcaca ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg
    26101 tatcggcgtt tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt
    26161 cgtgagtatt ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg
    26221 ataaaaacag ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa
    26281 tcagtttgtg cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg
    26341 aagtaatccc tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg
    26401 cgcaagccac ggcaataatg gcggggatcc tgccattgcc tgaaggtgcg gcagaaaaag
    26461 cccgcacgca aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc
    26521 ttcaactgtt gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta
    26581 aagccttcca gcaacagctg gcagaggtgg cgattgccgg tttccagccg cagtttaata
    26641 aatgggtaga gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtggtgctct
    26701 ctgatgcgat gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct
    26761 ggctatatag cagtaaacct tatgcgcttg caacgccgcc gctttcggtg attaaccaat
    26821 ggcagcaggc gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc
    26881 atccgcaata tgcggtgatg catgaatcgt tactggcctt actcagtgac accaaaccgt
    26941 ggccccaact gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg
    27001 cgttgcgtga aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac
    27061 ctggcgatga cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag
    27121 cagaaactaa gccgatggat aagcaaacga cgtctcgtag taaacctgcg cctgccgttc
    27181 gcgccgccta cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat
    27241 tgggggcaga tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc
    27301 agcgtgctgg cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt
    27361 ctaccgggat catggttaac attccggcct attcgctggt ctactatcag aacggcaatc
    27421 aggtgctgga ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca
    27481 gtgcccttaa caatgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca
    27541 aagatattct gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg
    27601 tgatgcgcgg ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa
    27661 tcacggcctc gaatttaccg tttcgcttcc aacaggctcc aggcccacgg aactcgctgg
    27721 ggcgctataa attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc
    27781 acaatctgtt caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag
    27841 cttccgatct ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg
    27901 atgcgctgaa gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc
    27961 tctactacct gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt
    28021 acaattatga tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa
    28081 tcaggtaaat gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc
    28141 aattctctgc agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc
    28201 ggttaaggtg cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact
    28261 tgattatcat ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg
    28321 cactcggtgc cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc
    28381 gcattttgac actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg
    28441 gcagaggcta tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg
    28501 cgaacaaaat aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt
    28561 tattaggcac ccgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca
    28621 atgaactacg cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg
    28681 cgatggattt ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta
    28741 tgcgcgcagg tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc
    28801 cagcacggca ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat
    28861 tccggtcacc gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc
    28921 cgcactggtc gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg acgccagcgg
    28981 cctgacactg atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc
    29041 ggaactggcg caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg
    29101 gctgcaaggc ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac
    29161 gccagatcgt tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt
    29221 gttacattgc cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct
    29281 gctgatttct ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg
    29341 cgatcataat caactgattt cttcaatcaa agataaattg ctgccactgg gtgatgacgt
    29401 gacatttatt ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc
    29461 cttcctgcaa gacgaaatgc ccgtctggta aggcacacat aaaaaagccc gcttttcagc
    29521 gggcttcatt gtttttaacg cttacagcac tgccacaatc gcttcgcaca gcggagccat
    29581 gttatctggt gtcatcccgg ccacgtttac gcgaccagaa gcaacagcat acacgccaaa
    29641 ctcttcgcgc agacgcagca cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt
    29701 ctgtttgatg ataaagctga agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa
    29761 caactgacgc atacgctgaa tacgctggcg catatcagtc agctcttgtt cccaaatcgc
    29821 acgtaacgca tcgttgctca ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt
    29881 agagtagtta gcgcgaatcg ccgctttcat ttggctgaat gcgcgatcaa cggtttcact
    29941 gtcggcagca accagagtac aagcgccaac acgctcgttg tacaggccaa agtttttaga
    30001 gtaggaactg gcaacaatca gctctttatg catagccgcg aaagcgcgca gtccttcagc
    30061 atcttcttcc agaccacggg caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc
    30121 tttctcaacg gagagttgtg ccagtgtctg ccattgttcc agcgtagggt cgataccggt
    30181 tgggttgtgg cagcagccat ggaacagcac tacgtcgcca gcctgagctt cgttcaggct
    30241 gttaatcagt gcatcgaagt caagggtgtg gttttccgca tcataataag cgtattcacg
    30301 aacttccaga tctgcagagt taaagacgct cttatggttc ggccagcttg ggttgctcac
    30361 ccacactcgc ttaacgctgg tattttttgc caggaaatcg gcagctatgc gtagtgcgcc
    30421 agtgccaccc ggagtctgtg ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt
    30481 accaaacagc agttcctgag tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta
    30541 atttttggtg gtttcatttt cgagcagata ctgttcagcc tttttcacgc tggtcagtac
    30601 cggggttttg cccgtctcat ctttatagac accaatcccg aggttaattt tgccgggacg
    30661 ttcatcggca cgaaacagat cggccaggcc cagaatcggg tcggcaggag cggcggtaat
    30721 gttctcaaac atgacgaggt tccattatgg ttacagaagg gaagtccgct atcagggtaa
    30781 cgggagattt acaaaattcc aactattact gatgaaaacg caggctgttt ttgcaagacg
    30841 tgagattgct ctggaaggca taaaaaaaac aggaccaaag tcctgttttt tcggcattta
    30901 acaaagaggt gtgctattag aactggtaaa cgatacccac agcaacggtg tcgtctgaac
    30961 ctacgcccag tttgttgtca gaatcgatct ggttgatgat gtagtcaaca taggtggaca
    31021 tgtttttgtt gaagtagtag gttgcgccca cttcaaagta gttcaccaga tcaacatcac
    31081 cgataccttc tacgtctttc gctttagatt tggtgtaagc gatggacgga cgcagaccga
    31141 aatcgaactg gtattgcgca actaacagaa cgtcttgcgt tttgttggcg aagccgctga
    31201 tgtttgtaaa tttattagtg atcggcgtag cgttacgcgt ttcaccgtag ttcgctgcca
    31261 ggtagatgtt gttcgcatca tacttcagac cagtagccca ctgttcagct tttttaccgt
    31321 tgccaagaag ttgagcttct tgcaggttgg tacggtcagc tgcaccataa gcaccaacga
    31381 taccaaagcc ttcgtattcg tagctgatag aaccgccaac accgtcgccg ttagagcggc
    31441 gtgcagtgtc acgctcgttt ttacccaggt actgaacagc gaagttcagg ccatcaacca
    31501 gaccaaagaa gttggagtta cgataggtag caacgccgcc aacacgacca acgaagaagt
    31561 catcgctgta tgcagtatca ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat
    31621 cataaaccac accgtagtta cggccgtaat cgaaagaacc aacgtcagcg tatttaagac
    31681 ccgcgaatgc cagacgcgtt ttgttaccag tttgagcgtc agcgccttca gagttgttac
    31741 cctggaagtt atattcccac tgaccataac cggtcagatc ggaattgatt tgagtttccc
    31801 ctttaaaacc aagacgggca taggtcatgt cgccattgcc accgtaactg ttttcaccgt
    31861 tacccttgga gaaataatgc agaccgacag ctttaccgta cagatctact ttgttgccat
    31921 ctttgttata gatttctgca gcgtttgcag tacctgctac taacagagca gggacgatca
    31981 ctgccagaat attgcgcttc atcattattt attaccctca tggttttttt tatgacacct
    32041 gccactgccg tcaataattt ctgtcaataa aattttacgg aactattgat gagagtttgg
    32101 tgtctttatg tgtctgcagg catctttcca ttcaaactaa cgtttcgcta ccgtgaaagt
    32161 gctacaaaga taaagattta gtttcaaaaa gaaaaaatat gtaactaaaa gtaa
//
LOCUS       DAZFDC010000044        32185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFDC010000044 DAZFDC010000000
VERSION     DAZFDC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.044"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            22..321
                     /locus_tag="ACHJ48_002122"
     CDS             22..321
                     /locus_tag="ACHJ48_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707681.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGGFPVTNIVLFLILIFFKKR"
     gene            complement(368..1255)
                     /gene="rarD"
                     /locus_tag="ACHJ48_002123"
     CDS             complement(368..1255)
                     /gene="rarD"
                     /locus_tag="ACHJ48_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO4707682.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1307..1774)
                     /gene="yigI"
                     /locus_tag="ACHJ48_002124"
     CDS             complement(1307..1774)
                     /gene="yigI"
                     /locus_tag="ACHJ48_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO4707683.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1939..2808
                     /gene="pldA"
                     /locus_tag="ACHJ48_002125"
     CDS             1939..2808
                     /gene="pldA"
                     /locus_tag="ACHJ48_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO4707684.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2941..4770
                     /gene="recQ"
                     /locus_tag="ACHJ48_002126"
     CDS             2941..4770
                     /gene="recQ"
                     /locus_tag="ACHJ48_002126"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO4707685.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4834..5454
                     /gene="rhtC"
                     /locus_tag="ACHJ48_002127"
     CDS             4834..5454
                     /gene="rhtC"
                     /locus_tag="ACHJ48_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO4707686.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5516..6136)
                     /gene="rhtB"
                     /locus_tag="ACHJ48_002128"
     CDS             complement(5516..6136)
                     /gene="rhtB"
                     /locus_tag="ACHJ48_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO4707687.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6247..7269
                     /gene="pldB"
                     /locus_tag="ACHJ48_002129"
     CDS             6247..7269
                     /gene="pldB"
                     /locus_tag="ACHJ48_002129"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO4707688.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     IHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7277..8077
                     /gene="yigL"
                     /locus_tag="ACHJ48_002130"
     CDS             7277..8077
                     /gene="yigL"
                     /locus_tag="ACHJ48_002130"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO4707689.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKEPLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8153..9052
                     /gene="bioP"
                     /locus_tag="ACHJ48_002131"
     CDS             8153..9052
                     /gene="bioP"
                     /locus_tag="ACHJ48_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO4707690.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8940..9893)
                     /gene="metR"
                     /locus_tag="ACHJ48_002132"
     CDS             complement(8940..9893)
                     /gene="metR"
                     /locus_tag="ACHJ48_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO4707691.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10129..12390
                     /gene="metE"
                     /locus_tag="ACHJ48_002133"
     CDS             10129..12390
                     /gene="metE"
                     /locus_tag="ACHJ48_002133"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO4707692.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYVQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSMTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12430..13245)
                     /gene="ysgA"
                     /locus_tag="ACHJ48_002134"
     CDS             complement(12430..13245)
                     /gene="ysgA"
                     /locus_tag="ACHJ48_002134"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO4707693.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13507..14268
                     /gene="udp"
                     /locus_tag="ACHJ48_002135"
     CDS             13507..14268
                     /gene="udp"
                     /locus_tag="ACHJ48_002135"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO4707694.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14409..15836
                     /gene="rmuC"
                     /locus_tag="ACHJ48_002136"
     CDS             14409..15836
                     /gene="rmuC"
                     /locus_tag="ACHJ48_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO4707695.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15931..16686
                     /gene="ubiE"
                     /locus_tag="ACHJ48_002137"
     CDS             15931..16686
                     /gene="ubiE"
                     /locus_tag="ACHJ48_002137"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO4707696.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16700..17305
                     /gene="ubiJ"
                     /locus_tag="ACHJ48_002138"
     CDS             16700..17305
                     /gene="ubiJ"
                     /locus_tag="ACHJ48_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO4707697.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALAGLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALIKRLEKLEAK"
     gene            17302..18942
                     /gene="ubiB"
                     /locus_tag="ACHJ48_002139"
     CDS             17302..18942
                     /gene="ubiB"
                     /locus_tag="ACHJ48_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO4707698.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19021..19290
                     /gene="tatA"
                     /locus_tag="ACHJ48_002140"
     CDS             19021..19290
                     /gene="tatA"
                     /locus_tag="ACHJ48_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO4707699.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19294..19809
                     /gene="tatB"
                     /locus_tag="ACHJ48_002141"
     CDS             19294..19809
                     /gene="tatB"
                     /locus_tag="ACHJ48_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO4707700.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19812..20588
                     /gene="tatC"
                     /locus_tag="ACHJ48_002142"
     CDS             19812..20588
                     /gene="tatC"
                     /locus_tag="ACHJ48_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO4707701.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20630..21412
                     /gene="tatD"
                     /locus_tag="ACHJ48_002143"
     CDS             20630..21412
                     /gene="tatD"
                     /locus_tag="ACHJ48_002143"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO4707702.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21409..21897)
                     /gene="rfaH"
                     /locus_tag="ACHJ48_002144"
     CDS             complement(21409..21897)
                     /gene="rfaH"
                     /locus_tag="ACHJ48_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO4707703.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22064..23557
                     /gene="ubiD"
                     /locus_tag="ACHJ48_002145"
     CDS             22064..23557
                     /gene="ubiD"
                     /locus_tag="ACHJ48_002145"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO4707704.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPIS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23603..24304
                     /gene="fre"
                     /locus_tag="ACHJ48_002146"
     CDS             23603..24304
                     /gene="fre"
                     /locus_tag="ACHJ48_002146"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO4707705.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24496..25659)
                     /gene="fadA"
                     /locus_tag="ACHJ48_002147"
     CDS             complement(24496..25659)
                     /gene="fadA"
                     /locus_tag="ACHJ48_002147"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO4707706.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25669..27858)
                     /gene="fadB"
                     /locus_tag="ACHJ48_002148"
     CDS             complement(25669..27858)
                     /gene="fadB"
                     /locus_tag="ACHJ48_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO4707707.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLALGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFER
                     VDVVVEAIVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28048..29379
                     /gene="pepQ"
                     /locus_tag="ACHJ48_002149"
     CDS             28048..29379
                     /gene="pepQ"
                     /locus_tag="ACHJ48_002149"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO4707708.1"
                     /translation="MESLASLYKNHIATLQERTRDALTRFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQASEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29379..29993
                     /gene="yigZ"
                     /locus_tag="ACHJ48_002150"
     CDS             29379..29993
                     /gene="yigZ"
                     /locus_tag="ACHJ48_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO4707709.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30032..31483
                     /gene="trkH"
                     /locus_tag="ACHJ48_002151"
     CDS             30032..31483
                     /gene="trkH"
                     /locus_tag="ACHJ48_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO4707710.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31495..32040
                     /gene="hemG"
                     /locus_tag="ACHJ48_002152"
     CDS             31495..32040
                     /gene="hemG"
                     /locus_tag="ACHJ48_002152"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO4707711.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7481 a         8148 c         8792 g         7764 t
ORIGIN      
        1 tcttgggtga atggattcta tatgggaaat ggtaactttt ggcagttgtt cttgggtaaa
       61 gaaccttaca gcaaaaaatc tgattttgtt aaggcatggt tattgtggtg ggctattacc
      121 atccttgttc tatttatttt aagcctgact acgacattaa atttaagaag tataggtata
      181 gggttaagtt ttgggttttg tcaaatcctt ggtagatata cgatgtctct aggatggagt
      241 aagtactggg gagttttggg gggatttcct gtaacaaata ttgttctttt tcttatctta
      301 atttttttca aaaaaagata aataagtcag tgggtaggct ctttaaacgg cctacccatg
      361 ttaattctta actttttcta cgctgcgtat aaatcgcatc catcacaaaa attgccagcg
      421 ccacccaaat aaaggtgaaa gtcaccatct tatcggcacc cggtttttca ccataaaacg
      481 tcacagccag caggaacatc agcgtcgggc caatgtactg gaaaaagcct aacgttgaga
      541 gacgaaggcg cgtggcagcg gcggtaaaac acaacagcgg tacggtagtg acaataccgg
      601 cggcgatcag cagtaaattc agcgacatcg ggttttgccc catatggctg gttgagctgt
      661 cggcaatagc aaacaggtaa atcgccgcca cgggcagcag ccacatggtt tcaattaaca
      721 tgccggtttg tgcttctacc gcaatcttct tgcgcaccag gccgtagaag gcaaaactaa
      781 atgccagccc cagcgcgata ataggtagcg aaccaaaagt ccacagctgg actaacacgc
      841 cacatatcgc cagaatcacc gccagccatt gcatccggcg gaatcgctcg ccgaggaaaa
      901 tcatccccag cacaatgttc accagcgggt taataaagta accaaggctc gcttccagca
      961 tatggtgatt gttcaccgcc cagataaaca gtagccagtt gccaccaatc agcacggcag
     1021 agactgccag cataaaaatt ttctgtggcg tctgaatcag cgtttttaaa taggaccact
     1081 ggcggcaaat gctcatcagc accaccataa agaaaaacga ccagatcacg cgatgcgtca
     1141 ggatctcatc ggcgggcacg tagtaaatca acttgaagta cgctggggct ataccccaaa
     1201 taaaataagc ggcaagagcg agtaatacgc cctgccgcgt ttgttttgca tccatcggga
     1261 atactcattt ttaattggta acagcagttt acctgctttt atgtcttcaa cctaccatat
     1321 aggtggcggt ggcactggca atataaagct gttcttcatt gtgtaattca acgcgggcga
     1381 cggcgacttt attgcctgca cgcaacaggc tactggtagc agtaaaacgc tcgcctctgc
     1441 ctgggcgcag ataatcgacg cgaagatcaa tggtccccat ccgcgatagc cgctggcgta
     1501 gttcatcttc actgatggtt tcgtggcggg ttaaggtact tcctacgcac accagaccgg
     1561 cggcgacatc cagcgccgac gcaatgaccc cgccgtgcaa aatgctttgc gcccagttgc
     1621 ccaccatcat tggctgattt ttaaaggcca gctgtgcgaa ctctttttcg taacgctcca
     1681 gttccatccc caatgcgcgg ttaaatggca tgtgataaac aaacatctca cccactaatt
     1741 tcagggcttg ttcagcggtc agtacggcag acatatcatc cttacacttc attggttaat
     1801 gaaatgttga ttttatgatt ctttgttgtt ggtttctact ttaggaaggg ataactaacg
     1861 gcaatggagt taagtatgta aaataccccg cagaaaaaca ttcaccttat caataattcg
     1921 ttacggagaa cacgaccgat gcggactctg cagggctggt tgttgccggt gtttatgttg
     1981 cctatggcag tatatgcaca agaggcaacg gtgaaagagg tgcatgacgc gccagcggtg
     2041 cgtggcagta ttatcgccaa tatgctgcag gagcatgaca atccgttcac gctctatcct
     2101 tatgacacca actacctcat ctacacccaa accagcgatc tgaacaaaga agcgattgcc
     2161 agttacgact gggcggaaaa tgcgcgtaag gatgaagtaa agtttcagtt gagcctggca
     2221 tttccgctgt ggcgtgggat tttaggcccg aactcggtgt tgggtgcgtc ttatacgcaa
     2281 aaatcctggt ggcaactgtc caatagcgaa gagtcttcac cgtttcgtga aaccaactac
     2341 gaaccgcaat tgttcctcgg ttttgccacc gattaccgtt ttgcaggttg gacgctgcgc
     2401 gatgtggaga tggggtataa ccacgactct aacgggcgtt cagacccgac ctcccgcagc
     2461 tggaaccgcc tttatactcg cctgatggca gaaaacggta actggctggt agaagtgaag
     2521 ccgtggtatg tggtgggtaa tactgacgat aacccggata tcaccaaata tatgggttac
     2581 taccagctta aaatcggcta tcacctcggt gatgcggtgc tcagtgcgaa aggacagtac
     2641 aactggaaca ccggctacgg cggcgcggag ttaggcttaa gttacccgat caccaaacat
     2701 gtgcgccttt atactcaggt ttacagcggc tatggcgaat cgctcatcga ctataacttc
     2761 aaccagaccc gtgtcggtgt gggggttatg ctaaacgatt tgttttgatg aacggttgag
     2821 tggttggcaa atctggaatc cagcatccag gattaccctc tcagagacta aaagcattgc
     2881 agtttctcgc gcaggcgctg aaaatagcgc ctgtttttat ttcaggcaat cggggtgaat
     2941 gtggcgcagg cggaagtgtt gaatctggag tccggagcta aacaggtttt acaagaaacc
     3001 tttggctacc aacagtttcg ccccggccag gaagaaatta tcgacactgt gctttccggt
     3061 cgcgattgcc tggtcgtcat gcccaccggt ggcggaaaat ccctttgcta tcaaattcct
     3121 gccttattgc taaacggcct taccgtggtt gtttcaccgc tgatttcgtt gatgaaagat
     3181 caggtggatc aactgcaagc caacggcgtg gcggcggcgt gccttaactc gacgcaaacc
     3241 cgcgaacagc aacttgaagt gatgacaggc tgccgcaccg ggcaaattcg tctgctttat
     3301 atcgccccgg aacgcctgat gctggataac tttcttgagc atctggcgca ctggaatccg
     3361 gtgttattag ccgttgatga agcgcactgt atctcccaat ggggccacga tttccgcccg
     3421 gaatatgccg cgctcggtca gttgcgccag cggttcccga cgctgccgtt tatggcgctg
     3481 accgccacag ccgacgacac cacgcgccag gatatcgtgc gcctgctggg gctgaacgat
     3541 ccgctgattc aaatcagcag ttttgaccgt ccgaatattc gctacatgct gatggagaaa
     3601 ttcaaaccgc tcgatcagtt gatgcgctac gtgcaggaac agcgcggtaa gtccggcatt
     3661 atctactgca acagccgggc gaaagtagaa gacaccgctg cgcgcctgca aagcaagggt
     3721 attagcgcgg cggcctatca tgccgggctg gaaaataatg tccgcgccga cgtgcaggag
     3781 aaattccagc gcgatgacct gcaaattgtg gtggcgacgg tggcgttcgg catgggcatc
     3841 aataaaccaa acgttcgctt cgtggtccac tttgatattc cgcgcaatat cgaatcctat
     3901 tatcaggaaa ccggtcgcgc cgggcgtgat ggcctgcctg cggaagcgat gttgttttac
     3961 gatccagctg atatggcgtg gctgcgccgt tgtctggaag agaagccgca ggggcagttg
     4021 caggatatcg agcgccacaa actcaatgcg atgggcgcgt ttgccgaagc gcaaacttgc
     4081 cgtcgtctgg tattgctgaa ctattttggc gaagggcgtc aggagccgtg cgggaactgc
     4141 gatatctgcc tcgatccgcc gaaacagtac gacggttcaa ccgatgctca gattgccctt
     4201 tccaccattg gtcgtgtgaa tcagcggttt gggatgggtt atgtggtgga agtgattcgt
     4261 ggtgctaata accagcgtat ccgcgactat ggtcatgaca aactgaaagt ctatggcatg
     4321 ggccgtgata aaagccatga acattgggtg agcgtgatcc gccagctgat tcacctcggc
     4381 ctggtgacgc aaaatattgc ccagcattct gccctacaac tgacagaggc cgcgcgcccg
     4441 gtgctgcgcg gcgaatcctc tttacaactt gccgtgccgc gtatcgtggc gctcaaaccg
     4501 aaagcgatgc agaaatcgtt cggcggcaac tatgatcgca aactgttcgc caaattacgc
     4561 aaactgcgta aatcgattgc cgatgaaagt aatgtcccgc cgtacgtggt gtttaacgac
     4621 gcaaccttga ttgagatggc tgaacagatg ccgatcaccg ccagcgaaat gctcagcgtt
     4681 aacggcgttg ggatgcgcaa gctggaacgc tttggtaaac cgtttatggc gctgattcgt
     4741 gcgcatgtcg atggcgacga cgaagagtag tcagcagcat aaaaaagtgc caggatgaag
     4801 actccgtaaa cgtttccccc gcgagtcaaa tgtatgttga tgttatttct caccgtcgcc
     4861 atggtgcaca ttgtggcgct tatgagtccc ggtcccgatt tcttttttgt ctctcagacc
     4921 gctgtcagtc gttcccgtaa agaagcgatg atgggcgtgc tgggtattac ctgcggcgtt
     4981 atggtttggg cagggattgc gttgcttggc ctgcatttga ttatcgaaaa aatggcctgg
     5041 ctgcatacgc tgattatggt tggcggtggc ctgtatctct gctggatggg ttaccagatg
     5101 ctgcgcggcg cgctgaaaaa agaggcggtt tctgcacctg cgccacaggt cgagctggcg
     5161 aaaagtgggc gcagtttcct gaaaggttta ctgaccaatc tcgctaatcc gaaagcgatt
     5221 atctactttg gctcggtgtt ctcattgttt gtcggtgata acgttggcac taccgcgcgc
     5281 tggggcattt tcgcgttgat tattgtcgaa acgctggcgt ggtttactgt ggtggccagc
     5341 ctgtttgccc tgccgcaaat gcgccgtggt tatcaacgtc tggcgaagtg gattgatggt
     5401 tttgccgggg cgttatttgc cggatttggc attcatttga ttatttcgcg gtaatgccag
     5461 acgcgtcttc agagtaagtc ggataaggcg tttacgccgc atccgacatt atttttcacg
     5521 catgcctcgc cgatgctaac agcgctccca ccagcataaa caacgagccg aaaatcttat
     5581 tcagcgcctt catctgcttt ggtcctttaa tccatagagc aatccgttga gcaagggtgg
     5641 cgtaaccgat catcacaata atatcgacca caatagtggt gacaccgagc acgatatact
     5701 gcatcagttg cggctgttgc ggcatgatga attgcggaaa tagcgccgcc agaaacacaa
     5761 tacttttggg attggtgaga ttcacaaaaa ctgcgcgctg gaacaaatgt cgacgcgatt
     5821 gagtagaggc cagcgattta agatcaattg cgccagcggc gcgccattgc tgtattccca
     5881 gccaaatcaa gtaagccgcg cctgcccact tcaacacttc aaacgcaatc actgagcggg
     5941 aaaatagcgt ccccaacccc acgccaacca gcacaatatg aatcgccagt ccggtctgaa
     6001 gcccggcaat agacgccacc gcgccgcgat aaccgtggtt gagcgaggtg gtcatagtgt
     6061 tgattgcacc agagcctggc gacaggctta aaatgattga tgtcagtagg taggcaaacc
     6121 accattctaa ggtcatgatg aactcccggt gtgtctattt ttgtgccaca atacgctact
     6181 gtcgcagcgt tgtgtcaggc acgctaaaaa aaacgatttt acgtggttta agaggcagat
     6241 tacccgatgt ttcagcagca aaaagactgg gaaacaagag aaaacgcgtt tgctgctttt
     6301 accatgggac cgctgactga tttctggcgt cagcgtgatg aagcagagtt tactggtgtg
     6361 gatgacattc cggtgcgctt tgtccgtttt cgcgcacagc accatgaccg ggtggtagtc
     6421 atctgcccgg ggcgtattga gagttacgta aaatatgcgg aactggccta cgatttgttc
     6481 catttggggt ttgatgtctt aatcatcgac catcgcgggc agggacgttc cggtcgcctg
     6541 ttagccgatc cgcatctcgg gcatgttaat cgctttaatg attatgttga tgatctggcg
     6601 gcattctggc aacaggaggt ccagcccggt ccgtggcgta aacgctatat actggcacat
     6661 tcgatgggcg gtgcgatctc cacattattt ctgcaacgcc atccaggtgt atgtgacgcc
     6721 attgcgctaa ctgcgccaat gtttgggatc gtgattcgta tgccgtcatt tatggcacgg
     6781 cagatcctca actgggccga agcgcatcca cgtttccgtg atggctatgc aataggcacc
     6841 gggcgctggc gcgcgttgcc gtttgctatc aacgtactga cccacagcag acagcgatat
     6901 cgacgtaact tacgcttcta tgctgatgac ccaacgattc gcgtcggtgg gccgacctac
     6961 cattgggtac gcgaaagtat tctggctggc gaacaggtgt tagccggtgc gggtgatgac
     7021 gccacgccaa cgcttctctt gcaggctgaa gaggaacgcg tggtggataa ccgcattcat
     7081 gaccgttttt gtgaactccg caccgccgcg ggccatcctg tcgaaggggg acgaccgctg
     7141 gtaattaaag gtgcgtacca tgagatcctt tttgaaaagg acgcaatgcg ctcagttgcg
     7201 ctccacgcca tcgttgattt tttcaacagg cataactcac ccagcggaaa ccgctctaca
     7261 gaggtttaaa tttcttatgt accaggttgt tgcgtctgat ttagatggca cgttactttc
     7321 tcccgaccat acattatccc cttacgccaa agaacccctg aagctgctca ccgcgcgcgg
     7381 catcaacttt gtgtttgcga ccggtcgtca ccacgttgat gtggggcaaa ttcgcgataa
     7441 tctggagatt aagtcttaca tgattacctc caatggtgcg cgcgttcacg atctggatgg
     7501 taatctgatt tttgctcata acctggatcg cgacattgcc agcgatctgt ttggcgtagt
     7561 caacgacaat ccggacatca ttactaacgt ttatcgcgac gacgaatggt ttatgaatcg
     7621 ccatcgcccg gaagagatgc gcttttttaa agaagcggtg ttcaaatatg cgctgtatga
     7681 gcctggatta ctggagccgg aaggcgtcag caaagtgttc ttcacctgcg attcccatga
     7741 acaactgctg ccgctggagc aggcgattaa cgctcgttgg ggcgatcgcg tcaacgtcag
     7801 tttctctacc ttaacctgtc tggaagtgat ggcgggcggc gtttcaaaag gccatgcgct
     7861 ggaagcggtg gcgaagaaac tgggctacag cctgaaggat tgtattgcgt ttggtgacgg
     7921 gatgaacgat gccgaaatgc tgtcgatggc ggggaaaggc tgcattatgg gcagtgcgca
     7981 ccagcgtctg aaagaccttc atcccgagct ggaagtgatt ggtactaatg ccgacgacgc
     8041 ggtgccgcat tatctgcgta aactctattt atcgtaatcg ttctttattt ggtcagttgt
     8101 caacctgata cttcgctaca atggatactc gttaatcaaa gagttttcca ttgtggcgct
     8161 acttatcatc accacgattc tgtgggcctt ctcctttagc ttttatggcg agtaccttgc
     8221 ggggcacgtc gatagctatt ttgcggtgct ggtgcgcgtt ggcctggcgg cactcgtttt
     8281 tctgccgttt ctgcgtaccc gtggcaatag cctgaaaaca gtcggcctgt atatgctggt
     8341 gggcgcgatg cagctcggcg tgatgtatat gctgagtttc cgcgcttatc tctacctgac
     8401 ggtttccgaa ctgctgctgt tcaccgtact gacgccgctc tacatcacgc tgatttatga
     8461 catcatgagt aagcgccgtc tgcgctgggg atatgctttt agtgccttgt tggcggtgat
     8521 tggtgccggg attattcgct atgatcaggt caccgaccat ttctggactg gcttgttgct
     8581 ggtgcaactc tccaatatca cttttgccat cggcatggtg ggttacaaac gcctgatgga
     8641 aactcgcccg atgccgcagc ataacgcctt tgcgtggttc tatcttggcg cgttcctggt
     8701 ggcggtaatt gcatggttct tgctgggaaa tgcgcagaaa atgccgcaaa ccacgttgca
     8761 atggggcatt ctggtgtttc ttggcgtggt ggcttccggg attggctact ttatgtggaa
     8821 ctacggcgca acccaagtag acgccggaac gctgggcatt atgaataata tgcacgttcc
     8881 ggcagggctg ctggttaacc tggctatctg gcaccaacag ccgcactggc caacgtttat
     8941 tacaggcgcg ctggtgatcc tggcctcact gtgggtgcat cgtaagtggg tcgctccgcg
     9001 ctcttcacaa acggcagatg atcgcaggcg tgattgcgcg ctgagcgaat aaacgcttcc
     9061 gtaactggct gacgctgctc gccatcgcgc acggcggcgt acagtcggct ccacaagcct
     9121 tcgcccaggg ttttggtcac caccagaccc tggcgctcaa aactctctac tacccaatgt
     9181 ggtagcgcgg caatacccat ccgcgcggca accatctgaa tcaacaataa ggtgttatcg
     9241 acgcttttca gcgacgggct aacgcctgcg ggctggagaa aatgccgcca gacatccagt
     9301 cggctacgct gcaccggata aatcaacagc gtttcgctgg cgagatcttc cggcgtgatt
     9361 cgcgttttcg ccgccagtgg atggtcaggt gctaacacca gacgcacttc atagtcgaac
     9421 atcggcgaat aatgcaggcc actacgcggc agaatatcgg aggtcatcac cagatccagc
     9481 tcgccctgct gcaacgcagg ctgcgggtca aacgttacgc ccgatttaaa atccatctcc
     9541 acctgcggcc agttcttatg gaaattttct aatgcgggcg tcagccactg aatacagcta
     9601 tggcactcaa tggcaatgcg caggcgcgtc tgttgcggtt cattgcaggc ttgcagcgcc
     9661 tggctaattt gcggcagcac ctggtttgcc agttgcaaca ggatttcccc ctgtggagta
     9721 aagcgtagcg gctggctctt acgcacaaat agccggaagc caaggcgttg ttccagatcg
     9781 ctaaactggt gagacagggc ggattgcgtt tgatgcaacg tcgccgcagc ggctgcgaga
     9841 gagccgcagt tccgcaacgc ttgtagcgtt ttcaggtgtt ttacttcgat catgaaagtc
     9901 cttcacttcg gcatgaataa tttgcgcttg aggaatatac agtaaccgcc aattatggat
     9961 gtgtaaacat ctggacggct aaaatccttc gtcttttaaa tttatggtgc gttggctgcg
    10021 tttctccacc ccggtcactt acttcagtaa gctcccgggg atgaataaac ttgccgcctt
    10081 ccctaaattc aaaatccata ggatttacat ataattagag gaagaaaaat gacaatattg
    10141 aatcacaccc tcggtttccc tcgcgttggc ctgcgtcgcg agctgaaaaa agcgcaagag
    10201 agttattggg cggggaactc cacgcgtgaa gaactgctgg cggtagggcg tgaattgcgt
    10261 gctcgtcact gggatcaaca aaagcaagcg ggtatcgacc tgctgccggt gggcgatttt
    10321 gcctggtacg atcatgtact gaccaccagt ctgctgctgg gtaacgttcc ggcgcgtcat
    10381 cagaacaaag atggttcggt agatatcgac accctgttcc gtattggtcg tggacgtgcg
    10441 ccgactggcg aacctgcggc ggcagcggaa atgaccaaat ggtttaacac caactatcac
    10501 tacatggtgc cggagttcgt taaaggccaa cagttcaaac tgacctggac gcagctgctg
    10561 gacgaagtgg acgaggcgct ggcgctgggc cacaaggtga aacctgtgct gctggggccg
    10621 attacctggc tgtggctggg gaaagtgaaa ggtgaacagt ttgaccgcct gagcctgctg
    10681 aacgacattc tgccggttta tcagcaagtg ctggcagaac tggcgaaacg tggcatcgag
    10741 tgggtacaga ttgatgaacc cgcgctggta ctggaactac cacaggcgtg gctggacgca
    10801 tacaaacccg cttacgacgc gctccaggga caggtgaaac tgctgctgac cacctatttt
    10861 gaaggcgtaa cgccaaatct cgacacgatt actgcgctgc ctgttcaggg gctgcatgtg
    10921 gaccttgtac atggtaaaga tgacgttgtt gaactgcaca agcgcctgcc ttctgactgg
    10981 ctgctgtctg cgggtctgat caatggtcgt aacgtctggc gcgccgatct tactgagaaa
    11041 tatgtgcaaa ttaaggacat tgtcggcaaa cgtgatttgt gggtggcatc ttcctgctcg
    11101 ttgctgcaca gccccatcga cctgagcgtg gaaacgcgtc ttgatgcaga agtgaaaagc
    11161 tggtttgcct tcgccctaca aaaatgccat gaactggcac tgctgcgcga tgcgctgaac
    11221 agtggtgaca cggcagctct ggcagagtgg agcgccccaa ttcaggcgcg tcgtcactct
    11281 actcgtgtac ataatccggc ggtagaaaag cgtctggcgg cgatcaccgc ccaggacagc
    11341 cagcgtgcga atgtctatga agtgcgtgct gaagcccagc gtgcgcgttt taaactgcca
    11401 gcgtggccga ccaccacgat tggttccttc ccgcaaacca cggaaattcg taccctgcgt
    11461 ctggatttca aaaagggcaa tctcgacgcc aataactacc gcacgggcat tgcggaacat
    11521 atcaggcagg ccattgttga acaggaacgt ttgggactgg atgtgctggt acatggcgag
    11581 gccgagcgta atgacatggt ggaatacttt ggcgagcacc tcgacgggtt tgtctttacg
    11641 caaaacggtt gggtacagag ctacggttcc cgctgcgtga agccaccgat tgttattggt
    11701 gacgttagtc gcccggcacc gattaccgtg gagtgggcga agtatgcgca atctatgact
    11761 gataaaccgg tgaaagggat gctgactggc ccggtgacca ttctctgctg gtcgttcccg
    11821 cgtgaagatg tcagccgtga aaccatcgcc aaacaaattg cgctggcgct gcgtgatgaa
    11881 gtggcggacc tggaagccgc aggaattggc atcatccaga ttgatgaacc ggcgctgcgc
    11941 gaaggtttac cgctgcgtcg tagcgactgg gatgcatatc tccagtgggg cgtggaggcc
    12001 ttccgtatca acgccgccgt ggcgaaagat gacacacaaa tccacactca catgtgttat
    12061 tgcgagttca acgacatcat ggattcgatt gcggcactgg acgcagacgt catcaccatc
    12121 gaaacctcgc gttctgacat ggagttgctg gagtcgttcg aagagtttga ttatccgaat
    12181 gaaatcggtc caggcgtcta tgacattcac tcgccaaacg taccgagcgt ggaatggatt
    12241 gaagccttgc tgaagaaagc ggcaaaacgc attccggcag agcgcctgtg ggtcaacccg
    12301 gactgtggcc tgaaaacgcg cggctggcca gaaacccgcg cggcactggc gaacatggtg
    12361 caggcggcgc agaacttgcg tcgggggtaa aatccaaacc gggtggtaat accacccggt
    12421 cttttctcat tacagcgact tcttcccacc atactgctta aaccattcca gcatacgctg
    12481 ccagccatct tctgcagatg cggcatgata gctcgggcga taatcagcgt tgaatgcatg
    12541 cccggcgtcc gggtacacga taatctctgc tttcgcatta gcagcccgca gcgcctggcg
    12601 catggtttca acgctctcct gcggaatact gttatccaga ccgccatata agccgagaac
    12661 cggcgcgtta agatcggttg cgatatcaac aggttgtttc ggtgaattca gcgacttatc
    12721 gcccgtcagt ttgccgtacc acgccactgc ggcttttagc tgtggattat gcgcggcata
    12781 cagccaagtg atacgtccac cccagcagaa tccggtgatc attaaacgat gaacatcgcc
    12841 gccgttgcgg gaagcccaac tggcgacatg atcgagatcg gccagcacct gcgagtcagg
    12901 cacttttgct accagaccgc taagcaacgt ggggatatcg gcaaaatcat tcggatcgcc
    12961 ttcgcggaag taaagttcag gtgcgatagc cagatacccc tccagcgcca gacggcgaca
    13021 aatgtcgcgg atatgttcat gcacgccaaa aatttcctgc actacaatga ccactggcag
    13081 tgggccatcg ctttgctttg gtctggcatg gtaagcaggc atgttatccc cttgtgaagg
    13141 gatagaggtg aagcccgcca caatcgcgtc gtccggggtc tgaacgatgg tcgaagcgag
    13201 aggcgatgca gcaggtgcaa atccagattg ttgtgttgtt gccatggtat tctccgtacc
    13261 cttataaaaa tgttgcgcaa tgttaactat agtcagcatg caacaaatca cattgcctga
    13321 atcggctcat cttttatgca gtcctgcaga atgaagagtg atttatgtga tttgcatcac
    13381 ctttggtggg taaatttatg caacgcattt gcgtcatggt gatgagtatc acgaaaaaat
    13441 gttaaaccct tcggtaaagt gtctttttgc ttcttctgac taaaccgatt cacagaggag
    13501 ttgtatatgt ccaagtctga tgtttttcat ctcggcctca ctaaaaacga tttacaaggg
    13561 gctacgcttg ccatcgtccc tggcgacccg gatcgtgtgg aaaagatcgc cgcgctgatg
    13621 gataagccgg ttaagctggc atctcaccgc gaattcacta cctggcgtgc agagctggat
    13681 ggtaaacctg ttatcgtctg ctctaccggt atcggcggcc cgtctacctc tattgctgtt
    13741 gaagagctgg cacagctggg cattcgcacc ttcctgcgta tcggtacaac gggcgctatt
    13801 cagccgcata ttaatgtggg tgatgtcctg gttaccacgg cgtctgtccg tctggatggc
    13861 gcgagcctgc acttcgcacc gctggaattc ccggctgtcg ctgatttcga atgtacgact
    13921 gcgctggttg aagctgcgaa atccattggc gcgacaactc acgttggcgt gacagcttct
    13981 tctgatacct tctacccagg tcaggaacgt tacgatactt actctggtcg cgtagttcgt
    14041 cactttaaag gttctatgga agagtggcag gcgatgggcg taatgaacta tgaaatggaa
    14101 tctgcaaccc tgctgaccat gtgtgcaagt cagggcctgc gtgccggtat ggtagcgggt
    14161 gttatcgtta accgcaccca gcaagagatc ccgaatgctg agacgatgaa acaaactgaa
    14221 agccaagcgg tgaaaatcgt ggtggaagcg gcgcgtcgtc tgctgtaatt ctcttctcct
    14281 gtctgaaggc cgacgcgttc ggccttttgt atttttgcgt agcgcctcgc aggaaatgcc
    14341 tttccaactg gacgtttgta cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag
    14401 gaggcgttgt ggatttctca atcatggttt acgcagttat tgcgttggtg ggtgtggcaa
    14461 ttggctggct gtttgccagt tatcaacatg cgcagcaaaa agccgagcaa ttagctgaac
    14521 gtgaagagat ggtcgcggag ttaagcgcgg caaaacaaca aattacccaa agcgagcact
    14581 ggcgtgcaga gtgcgagtta ctcaataacg aagtgcgcag cctgcaaagt attaacacct
    14641 ctctggaggc cgatctgcgt gaagtaacca cgcggatgga agccgcacag caacatgctg
    14701 acgataaaat tcgccagatg attaacagcg agcagcgcct cagtgagcag tttgaaaacc
    14761 tcgccaaccg tatttttgag cacagcaatc gccgggttga tgagcaaaac cgtcagagtc
    14821 tgaacagcct gttgtcgccg ctacgtgaac aactggacgg tttccgccgt caggttcagg
    14881 acagcttcgg taaagaagca caagaacgcc ataccctgac ccacgaaatt cgcaatctcc
    14941 agcaactcaa cgcgcaaatg gcccaggaag cgatcaacct gacgcgcgcg ctgaaaggcg
    15001 acaataaaac ccagggcaac tggggcgagg tagtattgac gcgggtgctg gaggcttccg
    15061 gtctgcgtga agggtatgaa tatgaaaccc aggtcagcat cgaaaatgac gcccgctcgc
    15121 ggatgcagcc ggatgtcatc gtgcggctgc cgcagggaaa agatgtggtg atcgacgcca
    15181 aaatgacgct ggtcgcctat gaacgctatt ttaacgccga agacgactac acccgcgaaa
    15241 gcgcgctaca ggaacatatc gcgtcggtgc gtaaccatat ccgtttgctg ggacgcaaag
    15301 attatcaaca gctgccgggg ctgcgaactc tggattacgt gctgatgttt attcccgttg
    15361 aacccgcttt tttactggcg cttgaccgcc agccggagct gatcaccgaa gcgttgaaaa
    15421 acaatatcat gctggttagc ccgactacgc tgctggtggc gctgcgcact atcgccaacc
    15481 tgtggcgtta tgagcatcaa agccgcaacg cccagcaaat cgccgatcgt gccagcaagc
    15541 tgtacgacaa gatgcgtttg ttcatcgatg acatgtccgc gattggtcaa agtctcgaca
    15601 aagcgcagga taactatcgg caggcaatga aaaaactctc ttcagggcgc ggaaatgtgc
    15661 tggcgcaggc agaagcgttt cgcggtttag gagtagaaat taaacgcgag attaatccgg
    15721 atttggctga acaggcggtg agccaggatg aagagtatcg acttcggtcg gttccggagc
    15781 agccgaatga tgaagcttat caacgcgatg atgaatataa tcagcagtcg cgctagccca
    15841 ttgggagtag ttaagccggg tagaaatcta gggcatcgac gcccaatctg ttacacttct
    15901 ggaacaattt tttgatgagc aggcattgag atggtggata agtcacaaga aacgacgcac
    15961 tttggttttc agaccgtcgc gaaggaacaa aaagcggata tggtcgccca cgttttccat
    16021 tccgtggcat caaaatacga tgtcatgaat gatttgatgt catttggtat tcatcgtttg
    16081 tggaagcgat tcacgattga ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg
    16141 gctggtggca ccggcgacct gacagcgaaa ttctcccgcc tggtcggaga aactggcaaa
    16201 gtggtccttg ctgatatcaa tgaatccatg ctcaaaatgg gccgcgagaa gctgcgtaat
    16261 atcggtgtga ttggcaacgt tgagtatgtt caggcgaacg ctgaggcgct gccgttcccg
    16321 gataacacct ttgattgcat caccatttcg tttggtctgc gtaacgtcac cgacaaagat
    16381 aaagcactgc gttcaatgta tcgcgtgctg aaacccggcg gccgcctgct ggtgcttgag
    16441 ttctcgaagc caattatcga gccgctgagc aaagcctatg atgcatactc cttccatgtg
    16501 ctgccgcgta ttggctcact ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa
    16561 tccatccgta tgcatcccga tcaggatacc ctgaaagcca tgatgcagga tgccggattc
    16621 gaaagtgtcg actactacaa tctgacggca ggggttgtgg cgctgcatcg tggttataag
    16681 ttctgacagg agaccggaaa tgccttttaa acctttagtg acggcaggaa ttgaaagtct
    16741 gctcaacacc ttcctgtatc gctcacccgc gctgaaaacg gcccgctcgc gtctgttggg
    16801 caaagtgctg cgcgtggagg taaaaggctt ttcgacgtca ttgattctgg tgttcagcga
    16861 acgccaggtt gatgtactgg gcgaatgggc aggcgatgct gactgcaccg ttatcgccta
    16921 cgccagtgtg ttgccgaaac ttcgcgatcg ccagcagctt gccgccctga ttcgcagtgg
    16981 tgagctggaa gtgcagggcg atattcaggt ggtgcaaaac ttcgttgcgc tggcaggtct
    17041 ggcagagttc gaccctgcgg aactgctggc cccttatacc ggtgatatcg ccgctgaagg
    17101 aatcagcaaa gccatgcgcg gaggcgcaaa gttcctgcat cacggcatta agcgccagca
    17161 acgttatgtg gcggaagcca ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt
    17221 ggcctggttt gcggaagaga cggctgccgt cgagcgtgct gttgatgccc tgatcaaacg
    17281 gctggaaaaa ctggaggcta aatgacgcca ggtgaagtac ggcgcctata tttcatcatt
    17341 cgcacttttt taagctacgg acttgatgaa ctgatcccca aaatgcgtat caccctgccg
    17401 ctacggctat ggcgatactc attattctgg atgccaaatc ggcataaaga caaactttta
    17461 ggtgagcgac tacgactggc cctgcaagaa ctggggccgg tatggatcaa gttcgggcaa
    17521 atgttatcaa cccgccgcga tctttttccg ccgcatattg ccgatcagct ggcgttattg
    17581 caggacaaag ttgctccgtt tgatggcaag ctggcgaagc agcagattga agctgcaatg
    17641 ggcggcttgc cggtagaagc gtggtttgac gattttgaaa tcaagccact ggcttctgct
    17701 tctatcgccc aggttcatac cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa
    17761 gtcatccgcc cggatatttt gccggtcatt aaagcagacc tgaaacttat ctaccgtctg
    17821 gctcgctggg tgccgcgttt gctgccggat ggtcgccgtc tacgcccaac cgaagtggtg
    17881 cgcgagtacg aaaagacctt gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc
    17941 attcagcttc ggcgaaattt tgaagacagc ccgatgctct acatcccgga ggtttaccct
    18001 gactattgta gtgaagggat gatggtgatg gagcgtattt acggcattcc ggtgtctgat
    18061 gttgcggcgc tggagaaaaa cggcaccaac atgaaattgc tggcggaacg cggcgtgcag
    18121 gtgttcttca ctcaggtctt tcgcgacagc tttttccatg ccgatatgca ccctggcaac
    18181 atcttcgtaa gctatgaaca cccggaaaac ccgaaatata tcggcattga ttgcgggatt
    18241 gttggctcgc taaacaaaga agataaacgc tatctggcag aaaactttat tgccttcttt
    18301 aatcgcgact atcgcaaagt ggcagagcta cacgtcgatt ctggctgggt gccaccagat
    18361 accaacgttg aagagttcga atttgctatt cgtacggtct gtgaacctat ctttgagaaa
    18421 ccgctggccg aaatttcgtt tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc
    18481 ttcaatatgg aagtgcagcc gcaactggtg ttactccaga aaaccctgct ctacgtcgaa
    18541 ggggtaggac gccagcttta tccgcaactc gatttatgga aaacggcgaa gcctttcctg
    18601 gagtcgtgga ttaaagatca ggtcggtatt cctgcgctgg tgagagcatt taaagaaaaa
    18661 gcgccgttct gggtcgaaaa aatgccagaa ctgcctgaat tggtttacga cagtttgcgc
    18721 cagggcaagt atttacagca cagtgttgat aagattgccc gcgagcttca gtcaaatcat
    18781 gtacgtcagg gacaatcgcg ttattttctc ggaattggcg ctacgttagt attaagtggc
    18841 acattcttgt tggtcagccg acctgaatgg gggctgatgc ccggctggtt aatggcaggt
    18901 ggtctgatcg cctggtttgt cggttggcgc aaaacacgct gattttttca tcgctcaagg
    18961 cgggccgtgt aacgtataat gcggctttgt ttaatcatca tctaccacag aggaacatgt
    19021 atgggtggta tcagtatttg gcagttattg attattgccg tcatcgttgt actgcttttt
    19081 ggcaccaaaa agctcggctc catcggttcc gatcttggtg cgtcgatcaa aggctttaaa
    19141 aaagcaatga gcgatgatga accaaagcag gataaaacca gtcaggatgc tgattttact
    19201 gcgaaaacta tcgccgataa gcaggcggat acgaatcagg aacaggctaa aacagaagac
    19261 gcgaagcgcc acgataaaga gcaggtgtaa tccgtgtttg atatcggttt tagcgaactg
    19321 ctattggtgt tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta
    19381 aaaacggtag cgggctggat tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa
    19441 ctgacccagg agttaaaact ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg
    19501 agcctcacta acctgacgcc cgaactgaaa gcgtcgatgg atgaactacg ccaggccgcg
    19561 gagtcgatga agcgttccta cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac
    19621 accatccata acccggtggt gaaagataat gaagctgcgc atgagggtgt aacgcctgcc
    19681 gctgcacaaa cgcaggccag ttcgccggaa cagaagccag aaaccacgcc agagccggtg
    19741 gtaaaacctg ctgcggacgc tgaaccgaaa accgctgcac cttccccttc gtcgagtgat
    19801 aaaccgtaaa catgtctgta gaagatactc aaccgcttat cacgcatctg attgagctgc
    19861 gtaagcgtct gctgaactgc attatcgcgg tgatcgtgat attcctgtgt ctggtctatt
    19921 tcgccaatga catctatcac ctggtatccg cgccattgat caagcagttg ccgcaaggtt
    19981 caacgatgat cgccaccgac gtggcctcgc cgttctttac gccgatcaag ctgaccttta
    20041 tggtgtcgct gattctgtca gcgccggtga ttctctatca ggtgtgggca tttatcgccc
    20101 cagcgctgta taagcatgaa cgtcgcctgg tggtgccgct gctggtttcc agctctctgc
    20161 tattttatat cggcatggcg ttcgcctact ttgtggtctt tccgctggca tttggcttcc
    20221 ttgccaatac cgcgccggaa ggggtgcagg tatccaccga catcgccagc tatttaagct
    20281 tcgttatggc gctgtttatg gcgtttggtg tctcctttga agtgccggtg gcaattgtgc
    20341 tgctgtgctg gatggggatt acctcgccag aagacttacg caaaaaacgc ccgtatgtgc
    20401 tggttggtgc attcgttgtc gggatgttgc tgacgccgcc ggatgtcttc tcgcaaacgc
    20461 tgttggcgat cccgatgtac tgtctgtttg aaatcggtgt cttcttctca cgcttttacg
    20521 ttggtaaagg gcgaaatcgg gaagaggaaa acgacgctga agcagaaagc gaaaaaactg
    20581 aagaataaat tcaaccgccc gtcagggcgg ttgtcatatg gagtacagga tgtttgatat
    20641 cggcgttaat ttgaccagtt cgcaatttgc gaaagaccgt gatgatgttg tagcgcgcgc
    20701 ttttgacgcg ggagttaatg ggctactcat caccggcact aacctgcgtg aaagccagca
    20761 ggcgcaaaag ctggcgcgtc agtattcgtc ctgttggtca acggcgggcg tacatcctca
    20821 cgacagcagc cagtggcaag ctgcgactga agaagcgatt attgagctgg ccgcgcagcc
    20881 agaagtggtg gcgattggtg aatgtggtct cgactttaac cgcaactttt cgacgccgga
    20941 agagcaggaa cgcgcttttg ttgcccagct acgcattgcc gcagatttaa acatgccggt
    21001 atttatgcac tgtcgcgatg cccacgagcg gtttatgaca ttactggagc cgtggctgga
    21061 taaactgcct ggtgcggttc ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg
    21121 cgtggcgcat ggaatttata tcggcattac cggttgggtt tgcgatgaac gacgcggact
    21181 ggagctgcgg gaacttttgc cgttgattcc ggcggaaaaa ttactgatcg aaactgatgc
    21241 gccgtatctg ctccctcgcg atctcacgcc aaagccatca tcccggcgca acgagccagc
    21301 ccatctgccc catattttgc aacgtattgc gcactggcgt ggagaagatg ccgcatggct
    21361 ggctgccacc acggatgcta atgtcaaaac actgtttggg attgcgtttt agagtttgcg
    21421 gaactcggtg ttcttcacac tgtgcttaat ctctttatta ataagattaa gcaatagcat
    21481 ggagcgagcc tcaccatcgg gttcggtgaa aatggcctga aagccttcga acgcgccttc
    21541 ggtaataatc accttatcac ccggataagg ggttgccgga tcgacaatgt ctttcggttt
    21601 atataccgat agctgatgaa tcaccgccga tgggactatc gctggcgacg cgccaaagcg
    21661 cacgaagtgg ctgacaccgc gggtcgcgtt gatagtcgtg gtatgaatca cttctggatc
    21721 aaattccaca aacaggtagt tggggaacaa tggctcactg actgcagtac gtttgccacg
    21781 cacgattttt tccagggtga tcatcggtgc caggcaattc acagcctgtc tttcgaggtg
    21841 ttcctgggca cgttgaagtt gcccgcgctt gcagtacagt aaataccagg attgcataat
    21901 gactcttatc cgtttaatcg gggcgcaagg atagcaaaag ctttacgcta agttaattat
    21961 attccccggt ttgcgttata ccgtcagagt tcacgctaat ttaacaaatt tacagcatcg
    22021 caaagatgaa cgccgtataa tgggcgcaga ttaagaggct acaatggacg ccatgaaata
    22081 taacgattta cgcgacttct tgacgttgct tgaacagcag ggtgagctaa aacgtatcac
    22141 gctcccggtg gacccgcatc tggaaatcac tgaaattgct gaccgcacgc tgcgtgctgg
    22201 tgggcctgcg ctgttgtttg aaaaccctaa agggtactca atgccggtgc tgtgcaactt
    22261 gttcggtacg ccaaagcgcg tagcgatggg tatgggccag gaagatgttt cagcactgcg
    22321 tgaagtcggt aaattattag catttctgaa agaaccagag ccgccaaaag gttttcgcga
    22381 tctgtttgat aagctgccgc agtttaagca ggtgttaaac atgccgacaa agcgactgcg
    22441 cggtgcaccc tgccaacaaa aaatcgtctc tggcgatgac gtcgatctca accgtattcc
    22501 cattatgacc tgttggccgg aagatgccgc gccgctgatt acatgggggc taaccgttac
    22561 acgtggccct cataaagagc gacagaatct gggcatttat cgccagcaac tgattggtaa
    22621 aaacaagctg attatgcgtt ggctgtcgca tcgcggcggc gcgctggatt atcaggagtg
    22681 gtgtgcggcg catccaggtg aacgtttccc gatctctgtg gcgttgggcg ctgatccggc
    22741 aaccattctc ggtgcagtca caccagtacc agatactttg tcggaatacg cctttgccgg
    22801 attgctacgt ggcaccaaaa ccgaagtagt gaagtgtatt tccaatgatc tcgaagtgcc
    22861 cgccagtgcg gagattgtgc tggaagggta tatcgaacaa ggcgaaatgg cgccagaagg
    22921 accgtatggt gaccacactg gttactataa cgaagtcgat agtttcccgg tatttaccgt
    22981 gacgcatatt acccagcgtg aagatgcgat ttaccattcc acctataccg ggcgtccgcc
    23041 agatgaaccc gcggtactgg gagtggcgtt gaacgaagta tttgttccca ttctgcaaaa
    23101 gcagttcccg gaaattgtcg atttttacct gccgccggaa ggctgctctt atcgcctggc
    23161 ggtagtgaca atcaaaaaac agtacgccgg acacgcgaag cgcgtcatga tgggcgtctg
    23221 gtcgttctta cgccagttta tgtacactaa atttgtgatc gtttgcgatg atgacgttaa
    23281 cgcacgcgac tggaacgatg tgatttgggc gattaccacc cgtatggacc cagcgcggga
    23341 tactgttctg gtagaaaata cgcctattga ttatctggat tttgcctcgc ctgtctccgg
    23401 gctgggttca aaaatggggc tggatgccac gaataaatgg ccgggggaaa cccagcgtga
    23461 atggggacgt cccatcaaaa aagatccaga tgttgtcgca catattgacg ccatctggga
    23521 tgaactggct atttttaaca acggtaaaag cgcctgatgc gcgtttgttt tgccctattt
    23581 atcgatccga cagagaaagc gcatgacaac cttaagctgt aaagtgacct cggtagaagc
    23641 tatcacggat accgtatatc gtgtccgcat cgtgccagac gcggcctttt cttttcgtgc
    23701 tggtcagtat ttgatggtag tgatggatga gcgcgacaaa cgtccgttct caatggcctc
    23761 gacgccggat gaaaaagggt ttatcgagct gcatattggc gcttctgaaa tcaaccttta
    23821 cgcgaaagca gtcatggacc gcatcctcaa agatcatcaa atcgtggtcg atattcccca
    23881 cggagaagcg tggctgcgcg atgatgaaga gcgtccgatg attttgattg cgggcggcac
    23941 cgggttctct tatgcccgct cgattttgct gacagcgttg gcgcgtaacc caaaccgtga
    24001 tatcaccatt tactggggcg ggcgtgaaga gcagcatctg tatgatctct gcgagcttga
    24061 ggcgctttcg ttgaagcatc ctggtctgca agtggtgccg gtggttgaac aaccggaagc
    24121 gggctggcgt gggcgtactg gcaccgtgtt aacggcggta ttgcaggatc acggtacgct
    24181 ggcagagcat gatatctata ttgccggacg ttttgagatg gcgaaaattg cccgtgacct
    24241 gttttgcagt gagcgtaatg cgcgggaaga tcgcctgttt ggcgatgcgt ttgcatttat
    24301 ctgagatata aaaaaacccg cccctgacag gcgggaagaa cggcaactaa aactttattc
    24361 agtggcattt agatctatgt cgcatccggc attcggcaca cgatgcctga tgcgacgctg
    24421 gcgcgtctta tcaggcctac attggtgccg gatcggtaga ccggataagg cgttcacgcc
    24481 gcatccggca agtggttaaa cccgctcaaa caccgtcgca ataccctgac ccagaccgat
    24541 acacatcgtc gccagaccaa actgaacgtc tttgtgttcc atcagattca gcagcgtggt
    24601 gctgatacgc gcaccggaac aacccagcgg atgacccagc gcgatcgcgc caccgttgag
    24661 gttgatcttc tcgtcaatct gctcaattag tcccagatct ttaatacatg gcaggatctg
    24721 cgcggcaaag gcttcattca tttcaaacac gccgatatca ctgacagaaa gccccgcttt
    24781 tttcagcgcc agcttcgagg ccggaaccgg gccgtaaccc atgatcgatg ggtcacaacc
    24841 aacgaccgcc atcgaacgca cacgagcgcg cggcttaaga cctaattcat gggcgcggct
    24901 ttcactcatc accagcatgg cagctgcgcc atcggaaagt gcagaagatg tgcccgccgt
    24961 taccgtaccg tttactggat caaacgccgg acgcagcgtg gcgagggctt ccacggtggt
    25021 ttccgggcga atcacttcgt cgtaattaaa ctgcttcagg acgccgtcgg catcgtgacc
    25081 accagttggg atgatttcat ttttaaatgc gcccgactgc gtggcggccc aggcgcgggc
    25141 gtgtgaccgc gcggcaaagg catcctgcat ttcacggctg ataccgtgca tacgcgccag
    25201 catttctgcc gttaagccca tcatgcccgc cgctttggcg acattgcggc tcaggccagg
    25261 gtgaaaatcg acgccgtgac tcatcggcac atggcccata tgctccacgc cgccaaccag
    25321 acatgcctgc gcatcgccgg tcatgatcat tcgtgctgcg tcatgcagtg cctgcatgga
    25381 tgaaccacac aagcgattga cagtaaccgc cgggacagag tgcggcactt ctgccagcag
    25441 cgccgcgtta cgggcgatat taaaaccctg ctccagcgtc tgctgcacac acccccagta
    25501 aatatcgtcg agggccgccg cttccagcgc cgggttacgc gccagcaggc tacgcattaa
    25561 atgagcggag agatcttctg cacgcacgtt acgaaaagca ccgcccttcg aacggcccat
    25621 cggggtgcga attgcatcga caatgacaac ctgttccatt gtgactcctt aagccgtttt
    25681 caggtcgcca accggacggg ctggctcaac cgggggatag tacggttcgt tatgacgcgc
    25741 tttattacgc agaccttccg gcacttcgta cagcgggccg aggtgctgat attgctgtgc
    25801 catatcgagg tattttgcgc tgccgagggt gtccagccag cggaacgcgc cgccgtggaa
    25861 cggagggaag cccaggccgt agaccagcgc catatccgct tctgccggag tggcgataat
    25921 gccttcttcc agacagcgca ccacttcgtt gaccatcggg atcatcatgc gggcgataat
    25981 ctcttcttcg ctgaaatcgc gcttcggctg gctcacttct gccagcaggt cgtcaacagc
    26041 ggcgtcttct tctttcttcg gcttaccttt gctgtcttct ttataatgcc agaaaccgag
    26101 gccgttcttc tgaccaaagc ggttggcatc aaacagcgcg tcgatggcat cgcgataatc
    26161 tttctgcatc cgctgtggga agcccgctgc catgactgcc tgggcgtgat gcgcggtgtc
    26221 aatgcccaca acgtccagca gatatgccgg acccataggc cagccaaact gtttttccat
    26281 cactttgtcg atcttgcgga aatccgcgcc gtcgcgcagc aactggctga aaccggcgaa
    26341 atacgggaac agcacgcggt tgacgaagaa gccggggcaa tcgttaacca caatcggcgt
    26401 tttacccatc ttgctcgccc aggcgacgac tttcgcaata gtttcgtcgg agcttttctc
    26461 gccgcgaata atttccacca acggcattcg gtggaccggg ttaaagaagt gcatcccgca
    26521 gaagttttcc ggacgttcca gcgcgttggc cagttcgctg ataggaatgg ttgaagtgtt
    26581 agacgccagc acggtatccg ggcgtacttt ctgctcggtt tctgccagca cggctttttt
    26641 cactttcggg ttttcaacaa tcgcttctac cacaacatcc acgcgctcaa atccggcgta
    26701 gtcgagcgtt gggtggatcg tggagatcac gccagccagt ttcagaccat cgatcttgcc
    26761 gcgctcaagc tgcttgttca gtagtttcgc ggcttcagtc atgccgaggg ctaacgactt
    26821 gtcgttaata tctttcataa tcaccggcac gcctttccac gcagactggt aagcgatgcc
    26881 gccacccata atgcctgcgc ccagcacggc ggcctgtttc ggggtttcaa cgtctttggt
    26941 gagtttcttc gctttgcctt ttacatattg atcgttaagg aaaatgccga ccagtgcgcg
    27001 agcttcgttg gtatgcgcca gcgggacaaa gcttttgttt tctaggttta aggcttcttc
    27061 acgaccaaaa cgagccgcag cttcaatggt ttttactgcg gtgatggggg ccgggtaatg
    27121 ttttcctgcc gtttgtgcga ccatcccttt agcgatggtg aagctcatgg tggcttcaat
    27181 cttgctcagt tttagcggtt ccagcttcgg ctgacgtttc gctttccagt cgaggtcgcc
    27241 gttaatggcc tggcgtaaaa tcgccatcgc cccttcaatc agtttttctg ctttgactac
    27301 gccatccacc agaccgattt tcagcgcctg atccgcgccg acatctttac cggcggcaat
    27361 gatttccagc gcactgtcag cgcccagcat acgtggcata cgtacagaac cgccaaagcc
    27421 cggcatgatg cccagtttgg tttccggcag accgatgcgc agatccggcg tcgccagacg
    27481 gtaatcggtc gccagcacgc attcgcagcc accgcccagc gcatagccat tgacggcagc
    27541 aatggtcggc accggcagat cttccaggcg attaaacacg ctattggcaa agtgcagcca
    27601 ctgacttaac tgttcttcag gaacgaggaa cagggacaaa aattcggtga tatcagcacc
    27661 gacgataaag gctgctttgt tcgaacgcag cagcagccct tttagatctg attgctgttc
    27721 cagcacgccg atggcctcgc cgaggctggc gacggtcgca gtgtcgagtt tattaactga
    27781 acctggggca tcaaatacca gttcggcaat gccatcttcc agccagtcaa ggtacagggt
    27841 gtcgcctttg taaagcatgt cagtctcctg aatccgcaag gtgatctggt cgtaccagat
    27901 gcatcgaagt gtgtattttg tgttaaaaat atgcaaataa aagattaaag aaatgccgat
    27961 ctgatcacgc tcggcagaaa tcacgctctg gatgaacgat gtgctaagat gcggagactt
    28021 aaggtcaaaa aaacagaagg gtaaaaaatg gaatcactgg cctcgctcta taaaaatcat
    28081 atagctacat tgcaggaacg aactcgcgat gcgctgacgc gcttcaagct ggatgcgtta
    28141 cttattcact ccggcgaact gttcaacgtt tttctcgacg atcatcccta tccgtttaaa
    28201 gtgaacccgc aattcaaagc gtgggtgccg gtaactcagg tgccaaactg ctggctgctg
    28261 gtggatggcg tgaataagcc gaaactgtgg ttctatctgc cggttgatta ctggcacaac
    28321 gtcgaaccgc tgccgacctc cttctggact gaagatgtag aagtgatcgc gctgccgaaa
    28381 gccgatggca ttggtagtct gttgcctgct gcgcgcggca atatcggtta tatcggtccg
    28441 gtgccggaac gtgcgctgca actgggtatt gaggccagca atatcaaccc gaaaggggtt
    28501 atcgactacc tgcattacta ccgctccttc aaaaccgagt acgagctggc ctgtatgcgt
    28561 gaagcgcaga aaatggcggt caacggtcat cgcgcggcag aagaagcgtt ccgttctggc
    28621 atgagcgagt tcgatatcaa tattgcctat ctgactgcga ccggtcatcg tgataccgac
    28681 gtaccttaca gcaacattgt ggcactcaac gaacacgctg cggtgctgca ttacaccaaa
    28741 ctggatcatc aggcgtcgga agagatgcgc agcttcctgc tggatgccgg ggccgaatat
    28801 aacggctatg ccgctgacct gacccgtacc tggtcggcaa aaagtgacaa cgattacgca
    28861 cagctggtga aagacgtaaa tgatgaacaa ctggcgctga tcgcgaccat gaaagctggc
    28921 gttagctatg tggattacca catccagttc catcagcgca tcgccaaatt gctgcgtaaa
    28981 catcaaatca tcaccgatat gagtgaagag gcgatggtcg aaaacgatct taccgggccg
    29041 tttatgccgc atggtatcgg ccatccgctg ggcctgcagg tgcatgacgt cgccggtttt
    29101 atgcaggatg atagcggtac gcacctcgcg gcaccggcaa aatatccgta cctgcgctgc
    29161 acccgtattc tccagccggg catggtgtta accatcgaac cgggtatcta cttcattgaa
    29221 tcgctgctgg caccgtggcg tgaagggcag ttcagcaagc acttcaactg gcagaaaatt
    29281 gaagcactga aaccgttcgg cggcattcgt atcgaagaca acgtggtgat ccacgaaaat
    29341 aacgtggaaa acatgacccg ggatctgaaa ctggcgtgat ggaaagctgg ttaattcctg
    29401 cggcaccggt cacggtcgtt gaagagatca aaaaaagccg cttcattacg ctgttggcgc
    29461 ataccgatgg cgttgaggcg gcgaaagcgt ttgttgaatc ggtgcgggca gaacaccccg
    29521 atgcccgcca ccattgcgtg gcgtgggtgg cgggtgcgcc ggatgattct caacagctgg
    29581 gtttctctga cgacggggag ccggcgggaa cggcaggtaa accgatgctc gcccagctaa
    29641 tgggcagcgg cgttggggaa attaccgctg tggtggtgcg ctactacggc ggcatattgc
    29701 ttggcaccgg tgggttagtg aaagcgtatg gcggcggcgt gaatcaggcg ctgcgccagc
    29761 taacgaccca acgcaagacg ccattaaccg aatatacttt gcaatgtgaa tatagtcagt
    29821 taaccggcat tgaagcgttg ctggggcagt gtgacggcaa aattatcaac agtgattatc
    29881 aggcattcgt tctgctgcgg gtggcgcttc cggcggcgaa agtggctgaa ttttccgcga
    29941 agctggcgga ttttagccgt ggttcattgc aattgttagc gattgaagaa taatccccac
    30001 ttcgttttgc agaactaagg aagcggcaga gatgcatttt cgcgccatta cccgaatcgt
    30061 tggactactg gtcatcttat tttcagggac catgattatc cctgggctgg tagcactcat
    30121 ctaccgggat ggagcgggcc gcgcttttac ccagaccttt tttgtcgccc tcgccattgg
    30181 ctctatgctg tggtggccga accgcaaaga gaagggcgaa ctgaaatccc gtgaggggtt
    30241 tctgatagtg gtgctgttct ggaccgtgct gggtagtgtc ggtgcgctcc cttttatctt
    30301 ctcggaaagc ccgaacctca cgattaccga tgcgtttttt gaatctttct ctggcctgac
    30361 caccaccggg gccactacgc tggtggggct ggattcgctc cctcatgcca tcctctttta
    30421 tcgccagatg ctgcaatggt ttggcgggat ggggatcatc gtgttagcgg ttgcgatact
    30481 gcctatcctc ggcgtgggtg ggatgcagct ctatcgcgca gaaatgcccg gcccgctgaa
    30541 agataacaaa atgcgcccgc gaattgcgga aacggcgaaa accctgtggt tgatttatgt
    30601 cttgctgacc gtcgcctgtg cgctggcgtt gtggtttgcc ggaatggatg cctttgatgc
    30661 catcggccat agctttgcga ctatcgctat tggcggcttc tcgacacatg atgccagtat
    30721 cggttatttc gatagcccga ctattaacac tatcattgct atcttcctgc tgatctccgg
    30781 ctgtaactac ggtctgcact tttcactgtt aagtgggcgt agtctgaagg tttattggcg
    30841 cgatccggaa tttcgcatgt ttatcggcgt acagtttacg ctggtggtta tttgtaccct
    30901 cgtactgtgg tttcataatg tctacagttc ggcgctgatg acaattaacc aggcgttttt
    30961 ccaggtggtg tcgatggcga caaccgccgg gtttacgact gacagcattg cccgctggcc
    31021 gctctttttg ccggtactgc ttttatgttc agcttttatc ggcggttgtg ccgggtcaac
    31081 gggcggtggc ctgaaagtga tccgcatcct gctgctgttt aagcagggga accgtgaact
    31141 gaaacgactg gtgcatccga acgccgtcta tagcattaag ctggggaatc gcgcactgcc
    31201 ggaacgtatc ctcgaagccg tttggggatt tttctccgcc tatgcattgg tgtttattgt
    31261 cagtatgctg gcgattatcg ccacgggcgt ggatgacttt tctgcctttg cctcggttgt
    31321 tgcgacattg aataacctgg ggccagggct tggcgtggtt gctgataact ttaccagtat
    31381 gaacccggtg gctaaatgga tcctgattgc caacatgctg tttggtcgtc tcgaggtctt
    31441 tacattgctg gtgctcttta ccccgacttt ctggcgtgaa tgatggagta atacgtgaaa
    31501 acattaattc ttttctcaac aagggacgga caaacgcgcg agattgcctc ctacttggct
    31561 tcggaactga aagaactggg gatccaggcg gatgtcgcca atgtgcaccg cattgaagaa
    31621 ccacagtggg aaaactacga ccgtgtggtc attggtgctt ctattcgcta tggtcactac
    31681 cactctgcgt tccaggaatt tgtcaaaaag catgcgacgc ggctgaattc gatgccgagc
    31741 gcgttttact ccgtaaacct ggtggcgcgt aaaccggaga agcgcactcc acagaccaac
    31801 agctacgcgc ggaagtttct gatgaactcg caatggcgtc ccgatcgctg cgcggtcatt
    31861 gccggggcgc tgcgttaccc acgttatcgc tggtacgacc gttttatgat caagctgatt
    31921 atgaagatgt caggcggtga aacggatacg cgcaaagaag ttgtctatac cgattgggag
    31981 caggtggcga atttcgcccg agaaatcgcc catttaaccg acaaaccgac gctgaaataa
    32041 gcataaagaa taaaaaatgc gcggtcagaa aattatttta aatttcccct tgtcaggccg
    32101 gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac gccgccgggt
    32161 cagcgggatt ctcctgagaa ttccg
//
LOCUS       DAZFDC010000045        32028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFDC010000045 DAZFDC010000000
VERSION     DAZFDC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.045"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..93)
                     /locus_tag="ACHJ48_002153"
                     /pseudo
     CDS             complement(31..93)
                     /locus_tag="ACHJ48_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            147..2132
                     /gene="pdeR"
                     /locus_tag="ACHJ48_002154"
     CDS             147..2132
                     /gene="pdeR"
                     /locus_tag="ACHJ48_002154"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO4707712.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTAY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(2114..2242)
                     /gene="ymiD"
                     /locus_tag="ACHJ48_002155"
     CDS             complement(2114..2242)
                     /gene="ymiD"
                     /locus_tag="ACHJ48_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO4707713.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            2280..2453
                     /gene="yciZ"
                     /locus_tag="ACHJ48_002156"
     CDS             2280..2453
                     /gene="yciZ"
                     /locus_tag="ACHJ48_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO4707714.1"
                     /translation="MSEFDAQRVAERIDIVLDILVADDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            2543..3292
                     /gene="yciT"
                     /locus_tag="ACHJ48_002157"
     CDS             2543..3292
                     /gene="yciT"
                     /locus_tag="ACHJ48_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO4707715.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            3561..3779
                     /gene="osmB"
                     /locus_tag="ACHJ48_002158"
     CDS             3561..3779
                     /gene="osmB"
                     /locus_tag="ACHJ48_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO4707716.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(3905..4231)
                     /gene="yciH"
                     /locus_tag="ACHJ48_002159"
     CDS             complement(3905..4231)
                     /gene="yciH"
                     /locus_tag="ACHJ48_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO4707717.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRSKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(4231..4968)
                     /gene="pyrF"
                     /locus_tag="ACHJ48_002160"
     CDS             complement(4231..4968)
                     /gene="pyrF"
                     /locus_tag="ACHJ48_002160"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO4707718.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(5161..6330)
                     /gene="lapB"
                     /locus_tag="ACHJ48_002161"
     CDS             complement(5161..6330)
                     /gene="lapB"
                     /locus_tag="ACHJ48_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO4707719.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(6337..6645)
                     /gene="lapA"
                     /locus_tag="ACHJ48_002162"
     CDS             complement(6337..6645)
                     /gene="lapA"
                     /locus_tag="ACHJ48_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO4707720.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLVRAERKIKRLENQLSPATDVAVVQHSSAAKE
                     "
     gene            complement(6794..7558)
                     /gene="pgpB"
                     /locus_tag="ACHJ48_002163"
     CDS             complement(6794..7558)
                     /gene="pgpB"
                     /locus_tag="ACHJ48_002163"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO4707721.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            7728..8318
                     /gene="ribA"
                     /locus_tag="ACHJ48_002164"
     CDS             7728..8318
                     /gene="ribA"
                     /locus_tag="ACHJ48_002164"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO4707722.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(8382..11057)
                     /gene="acnA"
                     /locus_tag="ACHJ48_002165"
     CDS             complement(8382..11057)
                     /gene="acnA"
                     /locus_tag="ACHJ48_002165"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO4707723.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLDLDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(11221..11316)
                     /gene="ymiC"
                     /locus_tag="ACHJ48_002166"
     CDS             complement(11221..11316)
                     /gene="ymiC"
                     /locus_tag="ACHJ48_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO4707724.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(11430..11597)
                     /gene="yciX"
                     /locus_tag="ACHJ48_002167"
     CDS             complement(11430..11597)
                     /gene="yciX"
                     /locus_tag="ACHJ48_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO4707725.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(11600..11740)
                     /gene="ymiA"
                     /locus_tag="ACHJ48_002168"
     CDS             complement(11600..11740)
                     /gene="ymiA"
                     /locus_tag="ACHJ48_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO4707726.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(12059..13033)
                     /gene="cysB"
                     /locus_tag="ACHJ48_002169"
     CDS             complement(12059..13033)
                     /gene="cysB"
                     /locus_tag="ACHJ48_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO4707727.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(13243..15840)
                     /gene="topA"
                     /locus_tag="ACHJ48_002170"
     CDS             complement(13243..15840)
                     /gene="topA"
                     /locus_tag="ACHJ48_002170"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO4707728.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            16220..16471
                     /gene="yciN"
                     /locus_tag="ACHJ48_002171"
     CDS             16220..16471
                     /gene="yciN"
                     /locus_tag="ACHJ48_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO4707729.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(16507..17556)
                     /gene="sohB"
                     /locus_tag="ACHJ48_002172"
     CDS             complement(16507..17556)
                     /gene="sohB"
                     /locus_tag="ACHJ48_002172"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO4707730.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVSTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            17776..18534
                     /gene="yciK"
                     /locus_tag="ACHJ48_002173"
     CDS             17776..18534
                     /gene="yciK"
                     /locus_tag="ACHJ48_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO4707731.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            18531..19121
                     /gene="cobO"
                     /locus_tag="ACHJ48_002174"
     CDS             18531..19121
                     /gene="cobO"
                     /locus_tag="ACHJ48_002174"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO4707732.1"
                     /translation="MSDERYQQRQQRVKEKVDARMAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDLVLLDELTYMVAYDYLPLEEVLQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(19161..20033)
                     /gene="rluB"
                     /locus_tag="ACHJ48_002175"
     CDS             complement(19161..20033)
                     /gene="rluB"
                     /locus_tag="ACHJ48_002175"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO4707733.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(20246..21829)
                     /locus_tag="ACHJ48_002176"
     CDS             complement(20246..21829)
                     /locus_tag="ACHJ48_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO4707734.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTSVD
                     VLRMQKFYLPRKKSSFYRPDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMIC
                     WLLPFLFCSLELLFARDDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMP
                     AGYFSAVFLTGYLTGMGYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGE
                     TRKLDYALLGWAVSANLGREWVARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTS
                     LLGRSYSGGAAGGGGGGGW"
     gene            complement(21857..22477)
                     /gene="yciO"
                     /locus_tag="ACHJ48_002177"
     CDS             complement(21857..22477)
                     /gene="yciO"
                     /locus_tag="ACHJ48_002177"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO4707735.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(22474..23355)
                     /gene="rnm"
                     /locus_tag="ACHJ48_002178"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(22474..23355)
                     /gene="rnm"
                     /locus_tag="ACHJ48_002178"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO4707736.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAHIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            23493..23537
                     /gene="trpL"
                     /locus_tag="ACHJ48_002179"
     CDS             23493..23537
                     /gene="trpL"
                     /locus_tag="ACHJ48_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO4707737.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    23514..23607
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            23629..25191
                     /gene="trpE"
                     /locus_tag="ACHJ48_002180"
     CDS             23629..25191
                     /gene="trpE"
                     /locus_tag="ACHJ48_002180"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO4707738.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVGPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLESRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            25191..26786
                     /gene="trpD"
                     /locus_tag="ACHJ48_002181"
     CDS             25191..26786
                     /gene="trpD"
                     /locus_tag="ACHJ48_002181"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO4707739.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEHLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            26787..28148
                     /gene="trpCF"
                     /locus_tag="ACHJ48_002182"
     CDS             26787..28148
                     /gene="trpCF"
                     /locus_tag="ACHJ48_002182"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO4707740.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVICDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLKMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFADGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLVAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            28160..29353
                     /gene="trpB"
                     /locus_tag="ACHJ48_002183"
     CDS             28160..29353
                     /gene="trpB"
                     /locus_tag="ACHJ48_002183"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO4707741.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            29353..30159
                     /gene="trpA"
                     /locus_tag="ACHJ48_002184"
     CDS             29353..30159
                     /gene="trpA"
                     /locus_tag="ACHJ48_002184"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO4707742.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADTLELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPKKMLAALKAFVQPMKAATRS"
     gene            30540..30719
                     /gene="yciG"
                     /locus_tag="ACHJ48_002185"
     CDS             30540..30719
                     /gene="yciG"
                     /locus_tag="ACHJ48_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4707743.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            30805..31305
                     /gene="yciF"
                     /locus_tag="ACHJ48_002186"
     CDS             30805..31305
                     /gene="yciF"
                     /locus_tag="ACHJ48_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4707744.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            31351..31857
                     /gene="yciE"
                     /locus_tag="ACHJ48_002187"
     CDS             31351..31857
                     /gene="yciE"
                     /locus_tag="ACHJ48_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4707745.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(31917..>32028)
                     /locus_tag="ACHJ48_002188"
     CDS             complement(31917..>32028)
                     /locus_tag="ACHJ48_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFO4707746.1"
                     /translation="YMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT         8119 a         8254 c         7730 g         7925 t
ORIGIN      
        1 cgtttgaaaa ctggcgtttt tccaaaacta gattacaaat aaatatataa aaaaaacaat
       61 gtgtttagcc gccgttaatt tcatgtaagt catgttgttc cttttattta accgcggact
      121 ccgctgttaa ccggaggata tgcatcatga aaaccgttag ggagtccaca acgttgtaca
      181 actttctcgg atcgcacaat ccatactggc ggttgacgga aagcagcgat gttttgcgct
      241 tttctaccac cgaaaccaca gaacctgaac gtacgttgca gttatctgcc gaacaggctg
      301 ctcgcatcag ggaaatgacg gtcatcacct ccagcctgat gatgagtctg accgtcgatg
      361 agagcgatct ttctgtgcat ctggtaggac gaaaaatcaa taaacgggaa tgggctggca
      421 acgcgtctgc atggcatgac acaccagcag ttgctcgtga tttatcacac ggactttcct
      481 ttgctgagca ggtagtttct gaagcacatt ccgcaatagt gattctcgac agccggggga
      541 atatccaacg cttcaatcgg ttatgtgaag attacacagg gttgaacgaa cacgacgtca
      601 ttggccaaag cgtgtttaaa ctgtttatga gccgtcgtga agctgcggca tccaggcgca
      661 ataaccgtgt attttttcga agcggcaatg catatgaagt cgaactgtgg ataccaacac
      721 gtaaaggcca gcggctgttt ctgtttcgca ataaatttgt ccacagcggc agtggcaaaa
      781 acgagatttt tttaatctgt tccggcaccg acattaccga agagcgccgc gctcaggaac
      841 gactgcgtat tctggcaaat accgacagta tcaccggact gccgaatcgt aacgcaatgc
      901 aggagttaat cgatcacgct attaatcagg cagataacaa taaagttggg gtcgtgtatc
      961 ttgatttgga taattttaaa aaggtcaacg acgcctatgg gcatttattt ggtgaccagt
     1021 tattacgcga cgtgtcattg gctattttaa gctgtctcga acatgaccag gtgttggcgc
     1081 gtccaggtgg cgatgagttt ctggtactgg catccaacac ctcacaaagc gcgctggaag
     1141 caatggcatc acgaattttg acccgcttac ggctcccctt tcgcattggt ttaattgaag
     1201 tttataccag ctgttcagta ggtattgcac tctctcccga acatggttcg gacagcgcgg
     1261 ctattattcg tcacgccgac acagcaatgt acacagcaaa ggaaggcgga cgaggacaat
     1321 tttgcgtttt taccccagaa atgaatcaac gggtatttga atatctctgg ctggatacca
     1381 acttgcgtaa agcactggaa aacgatcagt tggttattca ctatcaaccc aaaatcacct
     1441 ggcgtggcga agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt
     1501 tgattccacc gttggacttc atttcctacg cagaagagtc agggctaatt gtgcctttag
     1561 gccgttgggt gattctcgat gtcgtacgcc aggtggcgaa gtggcgggat aaaggcatta
     1621 acctgcgagt ggcggtaaac atttctgcac gtcagctcgc cgatcaaacc attttcaccg
     1681 ccctgaaaca ggttctccag gaactcaatt ttgaatactg ccctattgat gttgaattga
     1741 cagagagttg tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc
     1801 aactaggtgc gcaagtgcat ctggatgatt ttggtaccgc ctactcttca ctttcgcaac
     1861 tggcgcgctt tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca
     1921 aacaacctgt ctcgcagtca ctggttcggg caatcgtcgc tgtggcccag gcattgaatc
     1981 ttcaggtgat cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaatg
     2041 ggatcaatga gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac
     2101 gctggtataa acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat
     2161 attccgtcat tatatatcgt attcccggat gataattagc cgttgctgtt aaacacttct
     2221 cttttagcgg caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga
     2281 tgtctgaatt tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg
     2341 tggcagacga ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc
     2401 gccaggttgc tgaatctacg ccagacatcc cgaaggcacc gtgggagatt taacaatcgt
     2461 gccttcaggt tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta
     2521 taacgttata agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat
     2581 tgaccagggt caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac
     2641 cattcgccag gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt
     2701 tgcagtttcg cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa
     2761 gcgtgaactc gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga
     2821 aaatggcagc agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac
     2881 tatcatcacg gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagttat
     2941 tttgctcggt ggcgtgtacc agaaaaaaag cgaaagtatg gttggcccgt tgacacgcca
     3001 gtgcatccaa caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga
     3061 aactggattc accggtcgcg atatgatgcg taccgatgtg gtgaatgccg tgctggaaaa
     3121 agagtgcgaa gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc
     3181 catcggtccc gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga
     3241 tctgatgcat cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc
     3301 cttctctacg gatgatttgc agtttgacaa atcatccgct ctaagatgat tcctggttga
     3361 taattaagac tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata
     3421 taacaggaag tcattatcac ctgcgtgata taaccctgcg cgcgagcgga tttcacggaa
     3481 taatttcacc aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca
     3541 taaattcagg agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg
     3601 caattaccct ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca
     3661 ccgcaatcgg cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt
     3721 tgggtacatt aggtggcgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat
     3781 cgtaaataaa tgtcgattat cgactcgtaa ttataatacg cttgattctg ttcagtacga
     3841 aataactgaa taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt
     3901 tatgttaacc gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata
     3961 aatcacgctt atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc
     4021 attttttctt cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg
     4081 taatcaggca aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccacac
     4141 catcgccttt agagcgtact ggggccgctt tgggctcatc aatacgcccg gtttctgttg
     4201 agtagaccag acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat
     4261 cgctttcagc gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata
     4321 atcaacacca gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc
     4381 actcccctgc ggacgaatgc ccggcgtaac cagtttgaac tcctgaccga atacctgttt
     4441 aaagcgcaca gcttcctgag cagaacacac cacaccatca aggccacatt tttgcgtcag
     4501 tgctgccaga cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc
     4561 gctggcttcc atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa
     4621 cggaaccagt gcctcacgcg ctgcggtcat catacgcgcc ccaccagagg catgaacatt
     4681 caccatccac acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat
     4741 atcgtggaat ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa
     4801 ctgtggccca aacaatgtaa acatctcttt gccgaccttc agacgacaat cgcgtgggtc
     4861 gatcttgtcg acaaaggcca gcgcggcatc acgattatga taatcaaggg caacaaccac
     4921 aggagaattc gtaacagcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc
     4981 ttgatgatgg gcaccggaag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgga
     5041 aataaaaaat taacagttgc gatttctccc ctggcaggct ttttctgcgc cgccgttgcc
     5101 ggagcacaat gggacataaa taactagtat gttgtaacta aagtcggttt ttttaaaaaa
     5161 ttacaggcca tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca
     5221 atgccagtag agggtgtatg cggtaaaacc acatttctgg cagcgataac gaggcttact
     5281 acgtaccttc tcgccaacca tgtcacgcag caccatcagg ctctctttgg cacgcccttc
     5341 ttccgcttca tttaagtggt aatccattaa cttatggaac acacgcatgg tcggatgacg
     5401 ctgaagctgg cgcgtaatat agacctgtgc ggcctcacta ccgtcgcgcg cttcgatgat
     5461 atcagcaagc atcaattcag catcggcacc ggtgttctct tccaccgcgc gctgcaggaa
     5521 ttctgcccat tcggcagttt tacccaactg ctgatagcag gtttgcagca tttccagcgt
     5581 ttcgctgacc agttctctgt cctgggatat cacgcgttgc aaactttcga cggctttggc
     5641 gtattctcct ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt
     5701 atctgccgcc gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat
     5761 atgctgcagg gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc
     5821 cagcttcacc aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta
     5881 gatttgtagc aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt
     5941 gaacatgtct tccgcgcggt catataaccc ggcggccatg taatcacgcc ccagttgttg
     6001 aatcgccaac agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat
     6061 gcgaatagcg cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc
     6121 ttcaacggtg cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg
     6181 ttgattacta agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc
     6241 atcttgcttg ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac
     6301 aggcaaaagc agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg
     6361 ctgcactaca gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg
     6421 ttcagcgcgc accagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc
     6481 gatagcaaaa cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg
     6541 cgctaacaga taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat
     6601 cacgaagatc gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat
     6661 gtggttagag cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt
     6721 cgcgccgtct gcgaaatggc atccacgcgc attgttgatg atttaagggc taaattcgga
     6781 aaatcaacca gcgttaactt tcttgttctc gttgcgctat ttcgcgattt tcttccgcag
     6841 gtggtgttaa tggcccgcaa attcgttgcg caagccacgt tgccaccgcc accagtgccc
     6901 acgaaatcaa cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc
     6961 ttcccatgac tcccgttgcc cagaccagca agatagcaat ggttaacgtt cgccgacgcg
     7021 gccacagcaa accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag
     7081 gaaaggcaaa ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct
     7141 cttcagccaa ctgttctttc actagatttc cgcgttctgc tcgctttaaa gtgtagaact
     7201 catcaaccgg aatatgatgt gttttttcca gccagataac aaaaggtcgt ggttcctgga
     7261 ctttgtcttt gatccaggat ttaacgcctt gtcccacaag gattgcggcc gccagaatgg
     7321 caaataatac aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaata
     7381 aaatcaaatg tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg
     7441 cttttagtag ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta
     7501 ctggcataac aagcaatagt gcagctccca ctgcggtacg tcttgcaatc gaacgcatgg
     7561 cctctccttt ttgataagtc ccacaatcat aactgaaaat gccagttcca ggaaaaattg
     7621 acagatttgt gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt
     7681 tatggcaaaa taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac
     7741 gtgtggcaga agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag
     7801 aactggcaac cggacacgat catgtcgcgc tagtctatgg cgatatttcc gggcataccc
     7861 cggtacttgc gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct
     7921 gcgattgtgg cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta
     7981 ttttgctgta tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt
     8041 acgcactgca ggatcaaggt tacgatactg tagaggctaa ccaccagtta ggcttcgccg
     8101 ctgatgagcg cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag
     8161 tccgcttgtt aaccaataac ccgaaaaaag tcgaaattct gaccgaagcg gggattaata
     8221 ttgttgaacg cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata
     8281 ccaaagccga gaaaatgggc catttgctga acaaataacc ctcttgcatt gtgtaattaa
     8341 tttgcttgcc ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga
     8401 atgacataat gcaaaatgcc gtcgttctgg tagtaagtca actccgtcgc ggtgtcgata
     8461 cgacaacggc agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc
     8521 gtcgcgccgg gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc
     8581 cctaacgttt tacgcgttac gccttgcgga aattccagcg gcaggatgcc catgccaatt
     8641 aaattcgaac ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat accaagcaga
     8701 cgcggacctt ttgccgccca gtcacggctg gagcctgatc catactcttt cccggcaatc
     8761 accgccagcg gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg
     8821 tcgctgtcag gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg
     8881 atgcgaatat tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg
     8941 taggagttaa agtcttttcg ctcaacaccc cgaccttgta ggtatcgacc cgctgggctg
     9001 tcgggcttaa tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt
     9061 gcgaggatcc gcgcaccgtg aatatcttcc acaggtgctg gcgttgcctg catttcatca
     9121 aagaaaggcg ataaacgaat ataggttgaa tcctcctgcc atccgtaggt atcggatcgt
     9181 gcgacgttaa tttctttcca ctctgcagtg ccttcaaaga cttctgcgta ctctttgcgg
     9241 aacatttctg tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata
     9301 tctttcagat atattggatc gcctttacga tcatgaccaa taggctcaga agccaggttg
     9361 atattcatat ttcccgccag cgcataggca accaccagcg gcggcgaggc cagccagtta
     9421 gttttaacca gcggatggat acggccttca aagttacggt tgccggacag cactgcaccg
     9481 acggttaaat cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta
     9541 ccaatacagg tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt
     9601 gtcagtttcg cttttgccag atagtcagaa acgactttcg aacccggtgc cagcgacgct
     9661 ttgacccacg gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg
     9721 gccatcagca cacttgggtt agaagtgttg gtgcacgagg ttatcgcagc aatgaccaca
     9781 gcgccatcag gtaactgata ctgatgtccg ttcataacat aatcgaccgg ctggcgatct
     9841 ttatgcgtgg cattcacttc cagttcgtta ctggcggcaa atgcttttgg tacatcgggc
     9901 agtgcaacgc gatcctgtgg gcgtttaggc cctgccaggc tcgcttcaac gtcattcata
     9961 tccagatcta acgtactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc
    10021 gctttggcat atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg
    10081 taatcgaggg ttacagcatc gattgggaag aagccacagg tggcaccata ttctggcgac
    10141 atattggcaa tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat
    10201 tcgacgaatt tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg
    10261 tctgtggcgg taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg
    10321 ataagcatgg aaaccggctg gcctaacatt gcggcttctg cttcgatgcc acccacgccc
    10381 caccccagca cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta
    10441 tccggataag caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat
    10501 tcgaggttaa cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac
    10561 gcttgctttc cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt
    10621 acgttttctt caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg
    10681 accaggtcga ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc
    10741 gcttcgcgca ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc
    10801 acccttgccg ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc
    10861 agcgcgtgga tatcctcttc ggtaaccgag ttaccatcct gccagcgcag cagattttcg
    10921 agcaaaactt tgagtgactt gggtagacgg gtgatatcgc ccagtgattt ggcagcaagc
    10981 ggcaggctgt agtagtggta agttttatct ttggcctgca acgcgtcctt actggcttct
    11041 cgtagggttg acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc
    11101 agggtgaata ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga
    11161 taaaagagga gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga
    11221 tcagtgagta atgatccaga ggagttcggc ccagaagagc atcgacagag aaaatgcacc
    11281 catccaggac caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta
    11341 ctttgtgttg agataactca acctgttatt gctgcggtgt attaaaagcc ctttaattca
    11401 ttaaaaatgg gtaataataa tgatgataat tattgtttcg gctgatcatc ttcagcaaac
    11461 agttctataa aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa
    11521 cattcccgtt tcgtttcggg ttcattgtca ctatgctggc ataatggtga cgactccagt
    11581 tgctcctgac caaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc
    11641 agaaaagtgc agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcgc
    11701 ggcggggttc ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca
    11761 gcaagacgtt gaacgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt
    11821 tagtcaaaaa tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct
    11881 gaaatagata tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt
    11941 aatttaccct ataaatatga gggataaaaa tattgtgcac ggtatacaaa taagtaacaa
    12001 aagagaatat attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt
    12061 atttttccgg cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg
    12121 cgacagccgc atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat
    12181 acatataact gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga
    12241 agatatcgtg agcatcaaca cgcacaaggt ccggatcggc gaccggatcc accgccatgc
    12301 tggcaatgac ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg
    12361 ttgccgtgaa aacgatacgc ggcgttaacc ctgcgcgatt aaaggcagta tccagttctg
    12421 aacgtccagt aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg
    12481 taatggcttt tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt
    12541 ggtagcacgg taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga
    12601 aatctgcatt acctttagag acggcatcag caatttgtgt cggcgaaccc tggtgcatat
    12661 gcaaagaaac gcggggataa cgctcaataa agcctttgat gacgtttggt aacgcgtagc
    12721 gtgcctgggt atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac
    12781 cggcaaccga ttttatggca tcaactttcg acaggacttc gcgagcgata cgaattattt
    12841 cttgtcctgc tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc
    12901 ctagctcgtc ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc
    12961 cttccgctgt tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt
    13021 gttgtaattt catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac
    13081 acctggcggg tgactaatac gatttaataa taaaaaaggt gctaactata accactatat
    13141 catttatagc cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc
    13201 tttcgcgacc ctttgtttat aaaaacctga cagaattaaa ggttattttt tcccttcaac
    13261 ccatttgcca tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga
    13321 gacatattgc tgtttggttt tacggctaaa gcgaaccata gtcttattac cttccggatc
    13381 ctgctgcggc gcatcggcca gataacgcag tttttccggc agacgatcgc ggaagcgata
    13441 cagctcttcc accagcggcg cacgcgtttc acgcgatttc gggaaggtat tggccgccag
    13501 gaacacaccg gcagcaccgt cacgcagcac gaaataggca tctgattttt cgcacggcag
    13561 ctccggtaat ggtaccggat cttctttcgg tggagccact tcgccgttac gtaaaatctt
    13621 acgcgtgttt ttacactctt cgttggtgca ggccatatat ttaccgaaac gccccatttt
    13681 caggtgcatt tcagaaccac atttttcaca ctcaacgatc gggccgtcat aacctttaat
    13741 gcggaattcg ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg
    13801 caacttacgt ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tggggcaacg
    13861 acgttttgcg cgcagcgcgt tggtttcagc gtcttcgcct tccagcacgt tcagcacttc
    13921 gttttccggc accaggttaa tggttgtttt gcaacgctct ttcggcggca gcgcatagcc
    13981 agaacagcca aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaggt
    14041 cgggcagtcg atgctggtca gaaccatctg gttcggacgc atacccccct cttccggatc
    14101 tttttcagct ttatctaact gctgagtgaa atccgagaag aagtgatcca gtacagcttt
    14161 ccactctgct tcgtgatttg ccacctggtc aaggctgttt tccatctgcg cggtgaagtc
    14221 gtagttcatt aactcgcgga aattctcttc caggcgatcg gtgacgattt cgcccatttt
    14281 ttccgcatag aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat
    14341 gatcgacgca taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga
    14401 tgcttcactg aaacgggctg gcggcttggt aaagtgctgg gctggtgtca gttcaacgag
    14461 cgtcagagca tcgcctttat cgactgcagg taagatacga tcttcatcgc ctttacgcag
    14521 tgcaggcatc actttcgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg
    14581 gaaatcgccc gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca
    14641 ggcaacgaac tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt
    14701 cagcgattcc gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga
    14761 gttttctttg ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc
    14821 gctgatataa ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg
    14881 catgtaagtg atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac
    14941 gccaaatcca agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc
    15001 aggtttactg gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag
    15061 cagacttacc gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg
    15121 atgagtcacc tgcaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc
    15181 ccagaactct tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcggac
    15241 tgccaccgac tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg
    15301 cgaaaccata taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg
    15361 atcaatattc agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc
    15421 gttaaacacc actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca
    15481 tgcaatggct tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc
    15541 agccagttgt ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc
    15601 ctcccaattg tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt
    15661 tttaggcttt ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc
    15721 actggtcggc aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag
    15781 atacttgttg atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat
    15841 attcacctta cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact
    15901 ggcaacgagt tactgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct
    15961 gttcacgaaa attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga
    16021 cgaaattttg gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt
    16081 acctgataaa atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg
    16141 cgctcttgcc accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc
    16201 cttgtttaag gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt
    16261 gaaagaggcc aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac
    16321 cggcgtcaca cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca
    16381 aggattgcca acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt
    16441 gctttcagaa aaatatcatc tggttgatta aagcaaaacg cgaggtctta ccctcgcgtt
    16501 tgtcttttac atcaatggct tttgcccacg ctgccaccag cgtagcaata atcgatcggc
    16561 gctctctgcc gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac
    16621 attgaccact tctcggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc
    16681 atcaaccagg cccttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg
    16741 ttcaatatcc agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc
    16801 gttcagctct tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag
    16861 cgtacgctta tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa
    16921 gcggttaaag ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc
    16981 ggaaacaatt ttgtccgcca cacaggccat catgtaacca ccgctggcag caactttgtc
    17041 taccgtaaca gttaatggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa
    17101 cccgtaacca tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg
    17161 tttgaatgct gcaagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc
    17221 catgctgcct ttaaagtcca gcacccagac gcggggttta ctgtcagttg acacctcgcc
    17281 cagtttggct ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt
    17341 gtgccactgt ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc
    17401 cttatactgt tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg
    17461 agcaacattg acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt
    17521 cgccaaaaac aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac
    17581 gctttggctc acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc
    17641 gaaagtatga caaagtatcc attttgcggt gttatcgccc gaggctgatt gaaatgccct
    17701 tctgtttcag gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt
    17761 cgttgaggag tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc
    17821 ctggtgacgg gagccagtga tggtattggt cgtgaagccg cgatgacgta tgcacgctat
    17881 ggtgcgacag tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac
    17941 ataaacgaag aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcagg
    18001 tccgaagatt gccaacaact ggcacagcgc attgccgtta attatccgcg tctggatggt
    18061 gttttgcata atgccggatt actcggcgat gtttgtccaa tgagcgaaca aaatccacag
    18121 gtctggcagg acgtcataca ggtcaacgtt aatgccacct ttatgctcac ccaggcacta
    18181 cttcctttat tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga
    18241 cgtcagggac gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg
    18301 atgcaggtac tggccgatga atatcagcag cgcctgcgtg tcaactgcat taacccaggc
    18361 ggtacgcgca ccgcaatgcg tgccagcgcc ttcccgaccg aagatccaca gaaacttaaa
    18421 acacccgctg atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa
    18481 accggcatga cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg
    18541 aacgctacca acagcgtcag cagcgagtga aagaaaaagt agatgctcgt atggcccagg
    18601 cccaggatga acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg
    18661 cggcatttgg tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt
    18721 ttattaaagg cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt
    18781 ttcaggtgat ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg
    18841 cctgccgcga agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatttgg
    18901 ttttgcttga tgagctgact tatatggtgg cgtatgacta tttgccactg gaagaagtgt
    18961 tgcaggcgtt aaatgaacgt ccacatcagc agacggtgat tatcacgggt cggggctgtc
    19021 atcgggatat actcgaactg gcggatacgg taagtgagtt acgccccgtg aaacatgcgt
    19081 ttgatgctgg cgtaaaagcg cagataggga ttgattacta atctgtaaaa atgccggata
    19141 tgacgtccgg catacgttga ttaaccgtta ttacgcccac cagaacgacg tccgccgctc
    19201 acctgactgt gacgtttcac cgcacgacga atctgattcg ccttcatgcg acgacggtct
    19261 ttttctacag cgactttaga gctggtttct ggcggcaact ccaccagttc gcgcagatag
    19321 ttagtctggg caagatccag ctccgtccag ccgccgcgtg gcagaccttt cggcagtggg
    19381 atatcaccgt aacgaacacg gatcaggcgg cttacctgca cgccaaccgc ttcccacaga
    19441 cgacgaactt cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct
    19501 tcgccgccgc tgaacttgat ggttttaaag gctgccggac catcttccag ctgcacacca
    19561 cggcttaaat cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcatattca
    19621 cgttcaactt cacggcttgg atgcattaaa cggttagcca gttcaccgtc ggtggtgaac
    19681 agcaacagac cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcaccgcgc
    19741 agctttggca gacggtcaaa caccgttgga cgcccttccg gatcgttacg ggtacacaac
    19801 tcaccttccg gtttataata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc
    19861 gaaatcaggt gaccatcgat acggattttc aatccagggg taacttcaac gcgatcgccg
    19921 agtttagcaa ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca
    19981 cggcgagaac cgtggccggc acgcgccagc actttctgta gcttttcgct catagagctt
    20041 ccttttggtg tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact
    20101 tacatctgac tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact
    20161 cgacgtttta actggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag
    20221 cttgtttcga ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcagcac
    20281 caccggaata acttcgcccc aataaactgg tattggcccc cagactcagg tgcatctgta
    20341 atgagaacaa aacgccacta cgggcaattt ccggggcgcg aacagccgct gtgagtgatg
    20401 gggtgatacg tgctacccat tctcttccaa ggtttgctga gacagcccaa cctagcaacg
    20461 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
    20521 tacgcgcgag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttggg
    20581 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
    20641 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atatagagag
    20701 aataaaaggc cacgcctgaa caaatcagcg gtaaaaacaa agtgatgatt acacgattaa
    20761 cgcaaggctt gtcatcatcg cgggcaaaca aaagctcaag ggaacaaaat aaaaaaggaa
    20821 gaagccagca gatcataata aacatatcgc ccaccagact cattcctgcc cagcctacag
    20881 gactcaggtt accgtaaaga atgaccgcca atattgccag tccaccccat tgcaaaaaag
    20941 tatcaggccg gtaaaacgaa gactttttac gcggcagata aaatttctgc atccgtaaaa
    21001 catctaccga cgtaaattgc ggcctgcgct tccagagata taaccatcca caaaccagga
    21061 atagacacgg aaaccaaatt aacaacgatg acgtggaggg taaaagaaga tggctgaaaa
    21121 tattcgtcgt ttgtggagcc ggggcattgc taagtaacga gtgaggccaa cggtagagta
    21181 ccgtgaaatc ttcacgataa aacggatcgc tggattcaat tcttccgtcc tcaaggatgc
    21241 ggccatttcg atatgtgtcg ccctcttctc cagtaaagag atcgatttcg ctaaatggat
    21301 tacccgcgat atcagggaac ttgagtgaaa atcgggtctt atagatttca aacggccagt
    21361 ggttaccagt cacgttccag attaacagat ctgaatctcc ctcacgcagg aaagcatttt
    21421 taacctgata atgaatttga taatgatagt cgccttgagg caagaaacgt tgtttatcgc
    21481 ccacgacaat actcattaac ccgatatgcc agtcaagatg ccagggttca ggaataccat
    21541 cacgggtgac gccaacaata tgatagttca gcagtgcagc gtccccatca ggctgcatcc
    21601 atagtcgtgg taaatcacga taaaatccgt gcttaatctg ctgatttcgc gcctgaaaat
    21661 gaatattttc tttgatgtcg agcaccccat ccggttgcag agtggcttca acatcaaaga
    21721 gcgagatccc ttcttcataa gaaggcatct ccgtgttttt cgcataactt aacgaagaga
    21781 aaaaaagccc aactatcaac agcaaaatgc agcgaaactt ccctgccata gtcttccatc
    21841 cgtggaacgc aatttgttat aagaaaggct tcacatcacc tacgccttca cgcaccacga
    21901 ccggcgtatc atcggtaaga tcaataaccg ttgttggttt ctgcccgaga tagccgccgt
    21961 gaataatcaa atcgacctgt ttttccagac ggtctttgat ttcttcggga tcggattcgg
    22021 taaattcgct gcctggcagc attagcgaag tggaaagcat cggttcgccc agtgcttcaa
    22081 gcaacgcctg cgcaatcggg ttagaaggca cacgcatacc gatggttttg cgtttttcct
    22141 gcaacagacg gcgtggcacc tccttcgtcc ccttcaggat gaaggtatag ttgcccggcg
    22201 tgttgttttt cattaaacga aacgcaacgt tatcgacaaa tgaataggtc gagagttcag
    22261 aaagatcgcg acacatcagg gtaaagttgt gaccgtccgg cagctggcga atacgacaaa
    22321 tacgctccat ggcgttttta tcttcaattt tacagccgag cgcatagccg gaatcagttg
    22381 gataaacaat caccccgcct ttacgcacga tctccaccgc ctggttgatt agacgttgct
    22441 gcgggttatc aggatgaata taaaaaaact ggctcataat tccctctctg tggtgttctg
    22501 cggctgttcc catagctgcc agacgccttc aacgcctgcg ggcagccaga gtttacgacc
    22561 cagttcgatc cacgggcatg gctgatgaaa atcagatcct tgtgatgccc ataaatggtg
    22621 ctggcgggca agcgtcgcca gctgggtacg ttcattgggc gattgctggc actgcgcgac
    22681 ttccatcgcg tcgccgtcgt gttcggcaaa atgcgcaacc aatcttttca gccatttagc
    22741 actaagattg taccgcccag gatgagctag caccgcctta ccgccagaat gatgaatgac
    22801 atcaatagct tgttctattg tacaccactg tggcggaacg tatccggttt tcccgcgcgc
    22861 cagatacttt ttaaagacat ccgccattga actggctttg ccgcactcaa caaggaaacg
    22921 cgcaaaatga ccgcgcgtca ctgcgccgcc ctgcgccagt cgttgtgcgc cttccagcgc
    22981 cccaggtata tgcgcttttt caagtctttc ggcaatttgc tgcgcccgct gattgcgccg
    23041 ttctgtctgc tgcgcgagga actcacacat tagcggatga gtaatatcaa tattcagccc
    23101 gacaatatga atttcatgat tttcccagac cgtggaaatt tccacgccgg gaataagatt
    23161 cagcgccagt cccgaacgtg aaatttcctc tcttgctggc gcgattgcag ctgtggtgtc
    23221 atggtcggtg atcgccaggg tgccgacgcg catctcgact gcacggtgca ccaatgcttc
    23281 tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcgta
    23341 attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa
    23401 cggttctggc aaatattctg aaatgagctg ttgacaatca atcatcgaac tagttaacta
    23461 gtacgcaagt tcacgtaaaa agggtatcga caatgaaagc aattttcgta ctgaaaggtt
    23521 ggtggcgcac ttcctgaaac gggcagtgta ttcaccatgc gtaaagcaat cagataccca
    23581 gcccgcctaa tgagcgggct tttttttgaa caaaattaga gaataacaat gcaaacacaa
    23641 aaaccgactc tcgaactgct aacctgcgaa ggcgcttatc gcgacaatcc caccgcgctt
    23701 tttcaccaat tgtgtgggga tcgtccggca acgctgctgc tggaatccgc agatatcgac
    23761 agcaaagatg atttaaaaag cctgctgctg gtagacagtg cgctgcgcat tacagcttta
    23821 ggtgacactg tcacaatcca gtccctttcc ggcaacggcg aagccctgct gacactactg
    23881 gataacgccc tgcctgcggg tgtggaaaat gaacaattac caaactgccg tgtgctgcgc
    23941 ttcccccctg ttggtccact gctggatgaa gacgctcgct tatgctccct ttcggttttt
    24001 gacgctttcc gcttattgca gaatctgttg aatgtaccga aggaagaacg agaagccatg
    24061 ttcttcggcg gcctgttctc ttatgacctt gtggcggggt ttgaagattt accgcaactg
    24121 tcagcggaaa ataactgccc tgatttctgt ttttatctcg ctgaaactct gatggtgatt
    24181 gaccatcaga aaaaaagcac ccgcattcag gccagcctgt ttgctccgaa tgaagaagaa
    24241 aaacaacgtc tcactgctcg cctgaacgat cttcgccagc aactgaccga aaccgcgcca
    24301 ccgctgccgg tggtttccgt gccgcatatg cgttgtgaat gtaaccagag cgatgaagag
    24361 ttcggtggcg tagtgcgttt gttgcaaaaa gcgattcgcg ccggagaaat tttccaggtg
    24421 gtgccatctc gccgtttctc tctgccctgc ccgtcaccgc tggcggccta ttacgtgctg
    24481 aaaaagagta atcccagccc gtacatgttt tttatgcagg ataatgattt caccctgttt
    24541 ggcgcgtcgc cggaaagttc gctcaagtat gacgccacca gccgccagat tgagatctac
    24601 ccgattgccg gaacacgtcc acgcggtcgt cgcgccgatg gctcgctcga cagggacctt
    24661 gaaagccgta ttgaactgga aatgcgtacc gatcataaag agctttctga acatctgatg
    24721 ttggtggatc tcgcccgtaa tgacctggca cgcatttgca cccccggcag ccgctacgtc
    24781 gccgacctca ccaaagttga ccgttactct tacgtgatgc acctggtctc ccgcgtggtc
    24841 ggtgagctgc gccacgatct cgacgccctg cacgcttacc gcgcctgtat gaatatgggg
    24901 acgttaagcg gtgcgccgaa agtacgcgct atgcagttaa ttgccgaggc tgaaggtcgt
    24961 cgccgcggca gctacggcgg cgcggtaggt tatttcaccg cgcacggcga tctcgacacc
    25021 tgcattgtga tccgctcggc gctggtggaa aacggtatcg ctaccgtgca agccggtgct
    25081 ggcgtagtcc ttgattctgt tccgcagtcg gaagccgacg aaacccgtaa taaagcccgc
    25141 gctgtactgc gcgctattgc caccgcgcat catgcacagg agacgttcta atggctgaca
    25201 ttctgctgct cgataatatc gactctttta cttacaacct ggcagatcag ttgcgcagca
    25261 atggtcataa cgtggtgatt taccgcaacc atattccggc gcagacctta attgaacgcc
    25321 ttgcgacgat gagcaatccg gtgctgatgc tttctcctgg acccggtgtg ccgagcgaag
    25381 ctggttgtat gcctgaactg cttacccgcc tgcgcggtaa gctgcctatt attggcattt
    25441 gccttggtca tcaggcgatt gtcgaagctt acgggggcta tgtcggtcag gcgggcgaaa
    25501 ttcttcacgg taaagcgtcg agcattgaac atgacggtca ggcgatgttt gccggattaa
    25561 caaacccgct gccggtggcg cgttatcact cgctggttgg cagtaatatt ccggccggtt
    25621 taaccatcaa cgcccatttt aatggcatgg tgatggcggt gcgtcacgat gcagatcgcg
    25681 tttgcggatt ccagttccac ccggaatcca ttctcaccac ccagggcgct cgcctgctgg
    25741 aacaaacgct ggcctgggcg cagcagaaac tagagccaac caacacgctg caaccgattc
    25801 tggaaaaact gtatcaggcg cagaccctta gccagcagga aagtcatcag ctattttcag
    25861 cggtggtacg tggtgaactg aaaccggaac acctggcggc ggcgctggtg agcatgaaaa
    25921 ttcgcggcga gcacccgaac gagatcgccg gagcagcaac cgcgctactg gaaaacgccg
    25981 cgccgttccc gcgtccggat tatctgtttg ccgatatcgt cggcaccggc ggtgacggca
    26041 gcaacagcat caatatttcc accgccagtg cgtttgtcgc cgcggcctgt gggctgaaag
    26101 tggcgaaaca cggcaaccgt agcgtctcca gtaaatctgg ctcgtcggat ctgctggcgg
    26161 cgttcggtat taatcttgat atgaacgccg ataaatcgcg ccaggcgctg gatgagttag
    26221 gtgtatgttt cctctttgca ccgaaatatc acaccggatt tcgccatgca atgccggttc
    26281 gccagcaact aaaaacccgc accctgttca atgtgctggg gccattgatt aacccggcgc
    26341 atccgccgct ggcgttaatt ggtgtttata gtccggaact ggtgctgccg attgccgaaa
    26401 ccttacgcgt gctggggtat caacgcgcgg cggtggtgca cagcggcggg atggatgaag
    26461 tttcattaca cgcgccgaca atcgttgccg agctgcatga cggcgaaatt aagagctatc
    26521 aattgaccgc tgaagatttt ggcctgactc cctaccacca ggagcaactg gcaggcggaa
    26581 caccggaaga aaaccgtgac attttaacac gcttgttaca aggtaaaggc gacgccgccc
    26641 atgaagcagc cgtcgcggcg aatgtcgcca tgttaatgcg cctgcatggc catgaagatc
    26701 tgcaagccaa tgcgcaaacc gttcttgagg tactgcgcag tggttccgct tacgacagag
    26761 tcaccgcact ggcggcacga gggtaaatga tgcaaaccgt tttagcgaaa atcgtcgcag
    26821 acaaggcgat ttgggtagaa gcccgcaaac agcagcaacc gctggccagt tttcagaatg
    26881 aggttcagcc gagcacgcga catttttatg atgcgctaca gggtgcgcgc acggcgttta
    26941 ttctggagtg caagaaagcg tcgccgtcaa aaggcgtgat ctgtgatgat ttcgatccag
    27001 cacgcattgc cgccatttat aaacattacg cttcggcaat ttcggtgctg actgatgaga
    27061 aatattttca ggggagcttt gatttcctcc ccatcgtcag ccaaatcgcc ccgcagccga
    27121 ttttatgtaa agacttcatt atcgaccctt accagatcta tctggcgcgc tattaccagg
    27181 ccgatgcctg cttattaatg ctttcagtac tggatgacga acaatatcgc cagcttgccg
    27241 ccgtcgccca cagtctgaag atgggtgtgc tgaccgaagt cagtaatgaa gaggaactgg
    27301 agcgcgccat tgcattgggg gcaaaggtcg ttggcatcaa caaccgcgat ctgcgtgatt
    27361 tgtcgattga tctcaaccgt acccgcgagc ttgcgccgaa actggggcac aacgtgacgg
    27421 taatcagcga atcaggcatc aatacttacg ctcaggtgcg cgagttaagc cacttcgctg
    27481 acggttttct aattggttcg gcgttgatgg cccatgacga tttgcacgcc gccgtgcgtc
    27541 gggtgttgct gggtgagaat aaagtatgtg gcctgacgcg tgggcaagat gctaaagcag
    27601 cgtacgacgc gggcgcgatt tacggtgggt tgatttttgt tgcgacatca ccgcgttgcg
    27661 tcaacgttga acaggcgcag gaagtgatgg ctgcggcacc gttgcagtat gttggcgtgt
    27721 tccgcaatca cgatattgcc gatgtgctgg acaaagctaa ggtgttatca ctggtggcag
    27781 tgcaactgca tggtaatgaa gatcagctgt atatcgatac gctgcgtgaa gctctgccag
    27841 cacacgttgc catctggaaa gcattaagcg tcggtgaaac cctgcccgcc cgcgagcttc
    27901 agcacgttga taaatatgtt ttagacaacg gccagggtgg aagcgggcaa cgtttcgact
    27961 ggtcactatt aaatggtcaa tcgcttggca acgttctgct ggcggggggc ttaggcgcag
    28021 ataactgcgt ggaagcggca caaaccggct gcgccggact tgattttaat tctgctgtag
    28081 agtcgcaacc gggcatcaaa gacgcacgtc ttttggcctc ggttttccag acgctgcgcg
    28141 catattaagg aaaggaacaa tgacaacatt acttaacccc tattttggtg agtttggcgg
    28201 catgtacgtg ccacaaatcc tgatgcctgc tctgcgccag ctggaagaag cttttgtcag
    28261 cgcgcaaaaa gatcctgaat ttcaggctca gttcaacgac ctgctgaaaa actatgccgg
    28321 gcgtccaacc gcgctgacca aatgccagaa cattacggct ggcacgaaca ccacgctgta
    28381 tctgaagcgc gaagatttgc tgcacggcgg cgcgcataaa actaaccagg tgctcggtca
    28441 ggctttactg gcgaagcgga tgggtaaaac cgaaatcatc gccgaaaccg gggccggtca
    28501 gcatggcgtg gcgtcggccc ttgccagcgc cctgctcggc ctgaaatgcc gtatttatat
    28561 gggggccaaa gacgttgaac gccagtcgcc taacgttttt cgtatgcgtt taatgggtgc
    28621 ggaagtgatc ccggtgcata gcggttccgc gacgctgaaa gatgcctgta acgaggcgct
    28681 gcgcgactgg tccggcagtt atgaaaccgc gcactatatg ctgggcaccg cagctggccc
    28741 acatccttat ccgaccattg tccgtgagtt tcagcggatg attggcgaag aaaccaaagc
    28801 acagattctg gaaagagaag gtcgcctgcc ggatgccgtt atcgcctgtg ttggcggcgg
    28861 ttcgaatgcc atcggcatgt ttgcagattt catcaacgaa accaacgtcg gcctgattgg
    28921 tgtggagcct ggtggtcacg gtatcgaaac tggcgagcac ggcgcaccgc taaaacatgg
    28981 tcgcgtgggt atctatttcg gtatgaaagc gccgatgatg caaaccgaag acgggcagat
    29041 tgaagaatct tactccatct ccgccggact cgattttccg tccgtcggcc cacaacacgc
    29101 gtatcttaac agcactggac gcgctgatta tgtgtcaatt accgatgatg aagcactgga
    29161 agccttcaaa acgctgtgcc tgcacgaagg gattatcccg gcgctggaat cctcccacgc
    29221 cctggcccat gcgctgaaaa tgatgcgcga aaacccggaa aaagagcagc tactggtggt
    29281 taacctttcc ggtcgcggcg ataaagacat cttcaccgtt cacgatattt tgaaagcacg
    29341 aggggaaatc tgatggaacg ctacgaatct ctgtttgccc agttgaagga gcgcaaagaa
    29401 ggcgcattcg ttcctttcgt cacgctcggt gatccgggca ttgagcagtc gctgaaaatt
    29461 atcgatacgc taattgaagc cggtgctgac acgctggagt taggcatccc cttctccgac
    29521 ccactggcag atggcccgac gattcaaaac gccacactgc gtgcctttgc ggcaggtgtg
    29581 accccggcgc agtgctttga gatgctggca ctcattcgcc agaaacaccc gaccattccc
    29641 attggcctgc tgatgtatgc caacctggtg tttaacaaag gcattgatga gttttatgcc
    29701 cagtgcgaga aagtcggcgt cgattcggtg ctggttgccg atgtgccagt tgaagagtcc
    29761 gcgcccttcc gccaggccgc gttgcgtcat aatgtcgcac ctatcttcat ctgcccgcca
    29821 aatgccgatg acgacctgct gcgccagata gcctcttacg gtcgtggtta cacatatttg
    29881 ctgtcacgag cgggcgtgac cggcgcagaa aaccgcgccg cgttacccct caatcatctg
    29941 gttgcgaagc tgaaagagta caacgcggca cctccattgc agggatttgg tatttccgcc
    30001 ccggatcagg taaaagcagc gattgatgca ggagctgcgg gcgcgatttc tggttctgcg
    30061 attgttaaaa tcatcgagca acatattaat gagccaaaga aaatgctggc ggcactgaaa
    30121 gcttttgtac aaccgatgaa agcggcgacg cgcagttaat cccacagccg ccagttccgc
    30181 tggcggcatt ttaactttct ttaacgaagc cggaaaaatc ctaaattcat ttaatattta
    30241 tctttttacc gtttcgctta cccctgtcga acgtcaactt acgtcatttt tccgcccaac
    30301 agtaatataa tcaaacaatt taatcccgca acataacacc agtaaaatca ataattttct
    30361 ctaagtcact tattccgcag gcaattgtta atatatcaag aacgttcgtc ataatatatt
    30421 ttcctacttt cttctcgtta cgcttaattt gactaatcct cattagcgac taattttaat
    30481 gagtatcgac acacaacact catattaatg aaacaatgca acgcaacggg agaaataaca
    30541 tggccgaaca tcgtggtggt tcaggaaatt tcgccgaaga ccgtgagaag gcatccgacg
    30601 caggccgtaa aggcggtcag catagcggcg gtaattttaa aaatgatccg caacgcgcat
    30661 ctgaagcggg taaaaaaggc ggtcaacaaa gcggtggtaa taaatcagga aaatcctgat
    30721 tcaccgttaa ttattaatat cagctgataa agataattgc atctgcgggc cgtatggctc
    30781 gcagtgattt cactggagaa aaatatgaat atgaagacca ttgaagatgt atttattcac
    30841 ctgctttcag atacctacag cgcagaaaaa caattaaccc gggcactggc aaaactcgca
    30901 agagcaacat caaacgaaaa attaagtcag gcttttcatg cgcacctcga ggaaacacat
    30961 ggacagattg aacgtattga tcaagttgtg gaatcagaat cgaatctgaa aattaagcgc
    31021 atgaaatgtg tggcaatgga aggtcttatt gaagaagcta atgaggtcat cgaaagtacc
    31081 gagaaaaacg aagtgcgtga tgccgcactg attgccgcag cacagaaagt cgagcattat
    31141 gagattgcca gttacgggac attagcgacg ctggctgaac aattaggtta ccgtaaagca
    31201 gcgaagcttc tgaaagagac cctggaagaa gaaaaggcca ccgacatcaa actgactgat
    31261 ctggccctta ataacgtaaa taagaaagcc gaaaataaag cctgaaatat gaactttaac
    31321 ttttggtcat tttataaaga ggacattttc atgaatcgta ttgaacatta tcatgactgg
    31381 ttacgtgacg cccatgcaat ggaaaagcaa gccgaatcta tgcttgaatc catggccagc
    31441 cgtatagata attatcctga actacgtgct cgtattgaac aacatcttag tgaaaccaaa
    31501 aaccagattg ttcaactgga aactattctt gatcgtaatg acatttcacg ttcagtcatt
    31561 aaagattcca tgagtaaaat ggctgcgctt gggcagtcaa tcggtggtat attcccttct
    31621 gatgaaatag tcaaaggctc tattagcgga tatgtcttcg agcaatttga aatcgcctgt
    31681 tacacctctc tattagcagc agcaaaaaat gccggtgata cagcctcaat tccgatcatc
    31741 gaagcgattt taaatgagga aaagcaaatg gccgactggc tgattcagca tattccgcaa
    31801 acaactgaga aatttttaat tcgctctgaa actgatggcg tagaagcgaa gaaataatac
    31861 cctttatgcc atgtccttat tgaccccgta tattacgggg tcgtttttgt gcggaattaa
    31921 aaacgatatc ctgctgagaa cataaacacc cacggatcga ggcgtacgct atcatgttgc
    31981 tgtgcaccgc ccagtttata attggcggtg gtatcgatat ccatgtac
//
LOCUS       DAZFDC010000046        31180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFDC010000046 DAZFDC010000000
VERSION     DAZFDC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.046"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..599
                     /locus_tag="ACHJ48_002189"
     CDS             <1..599
                     /locus_tag="ACHJ48_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO4707747.1"
                     /translation="DSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVG
                     EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGT
                     IKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDN
                     IKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            829..1212
                     /gene="secE"
                     /locus_tag="ACHJ48_002190"
     CDS             829..1212
                     /gene="secE"
                     /locus_tag="ACHJ48_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO4707748.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1214..1759
                     /gene="nusG"
                     /locus_tag="ACHJ48_002191"
     CDS             1214..1759
                     /gene="nusG"
                     /locus_tag="ACHJ48_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO4707749.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1918..2346
                     /gene="rplK"
                     /locus_tag="ACHJ48_002192"
     CDS             1918..2346
                     /gene="rplK"
                     /locus_tag="ACHJ48_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO4707750.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2350..3054
                     /gene="rplA"
                     /locus_tag="ACHJ48_002193"
     CDS             2350..3054
                     /gene="rplA"
                     /locus_tag="ACHJ48_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO4707751.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3346..3843
                     /gene="rplJ"
                     /locus_tag="ACHJ48_002194"
     CDS             3346..3843
                     /gene="rplJ"
                     /locus_tag="ACHJ48_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO4707752.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3910..4275
                     /gene="rplL"
                     /locus_tag="ACHJ48_002195"
     CDS             3910..4275
                     /gene="rplL"
                     /locus_tag="ACHJ48_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO4707753.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4595..8623
                     /gene="rpoB"
                     /locus_tag="ACHJ48_002196"
     CDS             4595..8623
                     /gene="rpoB"
                     /locus_tag="ACHJ48_002196"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO4707754.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8700..12923
                     /gene="rpoC"
                     /locus_tag="ACHJ48_002197"
     CDS             8700..12923
                     /gene="rpoC"
                     /locus_tag="ACHJ48_002197"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO4707755.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13136..13579
                     /locus_tag="ACHJ48_002198"
     CDS             13136..13579
                     /locus_tag="ACHJ48_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFO4707756.1"
                     /translation="MKQEVEKWRPFKHPDGDVRDLSFLDAHQVIYVQHHEDKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKLSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVVFRAFSEIFHHCLKLIEK"
     gene            complement(14014..15147)
                     /gene="thiH"
                     /locus_tag="ACHJ48_002199"
     CDS             complement(14014..15147)
                     /gene="thiH"
                     /locus_tag="ACHJ48_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO4707757.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDERRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15144..15914)
                     /gene="thiG"
                     /locus_tag="ACHJ48_002200"
     CDS             complement(15144..15914)
                     /gene="thiG"
                     /locus_tag="ACHJ48_002200"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO4707758.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLAPQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15916..16116)
                     /gene="thiS"
                     /locus_tag="ACHJ48_002201"
     CDS             complement(15916..16116)
                     /gene="thiS"
                     /locus_tag="ACHJ48_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO4707759.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLVQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16100..16855)
                     /gene="thiF"
                     /locus_tag="ACHJ48_002202"
     CDS             complement(16100..16855)
                     /gene="thiF"
                     /locus_tag="ACHJ48_002202"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO4707760.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWGQGCYRCLWPENQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16848..17483)
                     /gene="thiE"
                     /locus_tag="ACHJ48_002203"
     CDS             complement(16848..17483)
                     /gene="thiE"
                     /locus_tag="ACHJ48_002203"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO4707761.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLSDYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17483..19378)
                     /gene="thiC"
                     /locus_tag="ACHJ48_002204"
     CDS             complement(17483..19378)
                     /gene="thiC"
                     /locus_tag="ACHJ48_002204"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO4707762.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGIADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19459..19559)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19611..20087)
                     /gene="rsd"
                     /locus_tag="ACHJ48_002205"
     CDS             complement(19611..20087)
                     /gene="rsd"
                     /locus_tag="ACHJ48_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO4707763.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20182..20955
                     /gene="nudC"
                     /locus_tag="ACHJ48_002206"
     CDS             20182..20955
                     /gene="nudC"
                     /locus_tag="ACHJ48_002206"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO4707764.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRYDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKHLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20995..22059
                     /gene="hemE"
                     /locus_tag="ACHJ48_002207"
     CDS             20995..22059
                     /gene="hemE"
                     /locus_tag="ACHJ48_002207"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO4707765.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVALYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22069..22740
                     /gene="nfi"
                     /locus_tag="ACHJ48_002208"
     CDS             22069..22740
                     /gene="nfi"
                     /locus_tag="ACHJ48_002208"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO4707766.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22783..23373
                     /gene="yjaG"
                     /locus_tag="ACHJ48_002209"
     CDS             22783..23373
                     /gene="yjaG"
                     /locus_tag="ACHJ48_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO4707767.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREADESNIGIIFQQ"
     gene            23560..23832
                     /gene="hupA"
                     /locus_tag="ACHJ48_002210"
     CDS             23560..23832
                     /gene="hupA"
                     /locus_tag="ACHJ48_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO4707768.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23845..24540
                     /gene="yjaH"
                     /locus_tag="ACHJ48_002211"
     CDS             23845..24540
                     /gene="yjaH"
                     /locus_tag="ACHJ48_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO4707769.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHFLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24542..24967)
                     /gene="zraP"
                     /locus_tag="ACHJ48_002212"
     CDS             complement(24542..24967)
                     /gene="zraP"
                     /locus_tag="ACHJ48_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO4707770.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25205..26581
                     /gene="zraS"
                     /locus_tag="ACHJ48_002213"
     CDS             25205..26581
                     /gene="zraS"
                     /locus_tag="ACHJ48_002213"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO4707771.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANCREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASLEGKGARFTLWLPVN
                     ITRKDPQG"
     gene            26578..27903
                     /gene="zraR"
                     /locus_tag="ACHJ48_002214"
     CDS             26578..27903
                     /gene="zraR"
                     /locus_tag="ACHJ48_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO4707772.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHIIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMLVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSLDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27900..29189)
                     /gene="purD"
                     /locus_tag="ACHJ48_002215"
     CDS             complement(27900..29189)
                     /gene="purD"
                     /locus_tag="ACHJ48_002215"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO4707773.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMASEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29201..30790)
                     /gene="purH"
                     /locus_tag="ACHJ48_002216"
     CDS             complement(29201..30790)
                     /gene="purH"
                     /locus_tag="ACHJ48_002216"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO4707774.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7322 a         8071 c         8535 g         7252 t
ORIGIN      
        1 tggattccta cattccggaa ccagagcgtg caattgacaa gccgttcctg ctgccgatcg
       61 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggta
      121 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
      181 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg ccgtgctggt gagaacgtag
      241 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
      301 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
      361 aaggcggccg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
      421 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
      481 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
      541 caatccgtga aggcggccgt accgttggcg cgggcgttgt agcaaaagtt ctgagctaat
      601 tgccgataac atttgacgca atgcgcacta aaagggcatc atttgatgcc ctttttgcac
      661 gctttcgtac cagaacctgg ctcatcagtg attttctttg tcataatcat tgctgagaca
      721 ggctctgttg agggcgtata atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg
      781 cgatcgcggg gtgaaaatgt ttgtagaaaa cttctgacag gttggtttat gagtgcgaat
      841 accgaagctc aaggaagcgg gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca
      901 ttgctcttgg tggcgattgt cggcaactat ctttatcgcg acattatgct gccgctgcgt
      961 gcgctggccg tagtaattct gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa
     1021 ggtaaagcta ccgttgcttt tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg
     1081 ccgactcgcc aggaaacatt gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg
     1141 tcactgatcc tgtggggact ggatggtatt ctggttcgcc tggtatcctt tatcactggc
     1201 ctgaggttct gagatgtctg aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc
     1261 cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat atcaaattac acaacatgga
     1321 agatttgttt ggtgaagtca tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca
     1381 gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc ctcgttcaga tggtgatgaa
     1441 cgacgcgagc tggcacctgg tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac
     1501 ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat gcgattatga accgcctgca
     1561 gcaggttggt gataagccgc gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt
     1621 taatgatggt ccgttcgctg acttcaacgg tgttgttgaa gaagtggatt acgagaaatc
     1681 tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag
     1741 ccaggttgaa aaagcctaac ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg
     1801 cgcgagattg gaatacaatt tcgcgccttt tgtttttatg ggtcttgtcc gtaaaacgat
     1861 tttttatatc acggggagcc tctcagaggc gttattaccc aacttgagga atttataatg
     1921 gctaagaaag tacaagccta tgtcaagctg caggttgcag ctggtatggc taacccgagt
     1981 ccgccagtag gtccggctct gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg
     2041 ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt
     2101 tacgctgacc gttctttcac tttcgttacc aagactccgc cggcagcagt tctgctgaaa
     2161 aaagcggctg gtatcaagtc tggttccggt aagccgaaca aagacaaagt gggtaaaatt
     2221 tcccgcgctc agctgcagga aatcgcgcag accaaagctg ccgacatgac tggtgccgac
     2281 attgaagcga tgactcgctc catcgaaggt actgcacgtt ccatgggcct ggtagtggag
     2341 gactaagaaa tggctaaact gaccaagcgc atgcgtgtta tccgcgagaa agttgatgca
     2401 accaaacagt acgacatcaa cgaagctatc gctctgctga aagagctggc gactgctaaa
     2461 ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg acgctcgtaa atctgaccag
     2521 aacgtacgtg gtgcaactgt actgccgcac ggtactggcc gttccgttcg cgtagccgta
     2581 tttacccagg gtgcaaacgc tgaagctgct aaagctgcag gtgctgaact ggtaggtatg
     2641 gaagatctgg ctgaccagat caagaaaggc gaaatgaact ttgacgttgt tattgcttct
     2701 ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc tgggtccgcg cggcctgatg
     2761 ccaaacccga aagtgggtac tgtaacgccg aacgttgctg aagcggttaa aaacgctaaa
     2821 gctggccagg ttcgttaccg taacgacaaa aacggcatca tccacaccac catcggtaaa
     2881 gtggactttg acgctgacaa actgaaagaa aacctggaag ctctgctggt tgcgctgaaa
     2941 aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga aagttagcat ctccaccacc
     3001 atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt
     3061 tacgtgggcg gtgattttgt ctacaatctt acccccacgt atgctaacga aagttagcag
     3121 ctaaaaagat ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag
     3181 tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta
     3241 tattctggct tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag
     3301 tgagttccag agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt
     3361 caagacaaac aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca
     3421 gtagttgcgg attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt
     3481 cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa
     3541 ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct
     3601 atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa
     3661 tttgaggtca aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc
     3721 ctggcaactc tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa
     3781 gcttcggctg gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct
     3841 taatcgcagt tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga
     3901 acaatttaaa tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta
     3961 atggacgttg tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct
     4021 gctgtagctg tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta
     4081 attctgaaag ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact
     4141 ggcctgggtc tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa
     4201 ggcgtgagca aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa
     4261 gttgaagtta aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg
     4321 tgacttttta gtcaccagcc tttttgcgct gtaaggcacc agtagcgttt cacactgttt
     4381 gactactgct gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga
     4441 cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg
     4501 aaagtgttcc attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca
     4561 gatgggtcga cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa
     4621 acgtattcgt aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc
     4681 tatccagctt gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct
     4741 ggaagctgct ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca
     4801 atacgtcagc taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg
     4861 cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc
     4921 gccggaaggc accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct
     4981 catgacagac aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct
     5041 gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa
     5101 agtgctgtat aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga
     5161 tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat
     5221 tctgcgtgcc ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat
     5281 ctttgaaatc cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga
     5341 aaccgcatcc tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat
     5401 cactgcgcgc cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt
     5461 tgagtacatc gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct
     5521 gatctgcgca gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg
     5581 tcacaagcgt atcgaaacgc tgttcaccaa tgatctggat cacggcccgt atatctctga
     5641 aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat
     5701 gatgcgccct ggcgagccgc cgactcgtga agcagcggaa agcctgttcg agaacctgtt
     5761 cttctccgaa gaccgttatg acctgtctgc ggttggtcgt atgaagttca accgttctct
     5821 gctgcgcgaa gaaatcgaag gttctggtat cctgagcaaa gacgacatca ttgatgttat
     5881 gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg
     5941 caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt
     6001 acgtgtagag cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc
     6061 tcaggatatg atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag
     6121 ccagctgtct cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg
     6181 tatctccgca ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga
     6241 cgtacacccg actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat
     6301 cggtctgatc aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac
     6361 tccgtatcgt aaagtgactg acggtgttgt aactgacgaa attcactacc tgtctgctat
     6421 cgaagaaggc aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt
     6481 cgtagaagac ctggtaacct gccgtagcaa aggcgaatcc agcttgttca gccgtgacca
     6541 ggttgactac atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc
     6601 gttcctggaa cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc
     6661 cgttccgact ctgcgtgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc
     6721 cgttgactcc ggtgtaactg cggttgctaa acgtggtggt gtcgttcagt acgtggatgc
     6781 ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga
     6841 catctacaac ctgaccaaat acacccgttc taaccagaac acctgtatta accagatgcc
     6901 gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac
     6961 cgacctcggt gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg
     7021 ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac
     7081 caccatccac attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccagaaga
     7141 gatcaccgct gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg
     7201 tatcgtttat attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggttacgcc
     7261 gaaaggtgaa actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa
     7321 agcgtctgac gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat
     7381 cgacgttcag gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga
     7441 agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc
     7501 gggtctgttc agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct
     7561 cgacaaattg ccgcgcgatc gctggctgga gctgggcctg accgacgaag agaaacaaaa
     7621 tcagctggaa cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact
     7681 cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat
     7741 tgttaaggta tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg
     7801 tcacggtaac aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga
     7861 aaacggtacg ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat
     7921 cggtcagatc ctcgaaaccc acttgggtat ggctgcgaaa ggtatcggcg acaagatcaa
     7981 cgccatgctg aaacagcagc aggaagtcgc gaaactgcgt gaattcatcc agcgtgcgta
     8041 cgatctgggc gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt
     8101 tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg
     8161 tgcgaaagaa gcagaaatta aagagctgct gaaacttggc gacctgccga cttctggtca
     8221 gatccgcctg tacgacggcc gcactggtga acagttcgaa cgtccggtaa ccgttggtta
     8281 catgtacatg ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg
     8341 ttcttacagc ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg
     8401 tttcggggag atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga
     8461 aatgctcacc gttaagtctg atgacgtgaa cggtcgtact aagatgtata aaaacatcgt
     8521 ggacggcaac catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga
     8581 gattcgttcg ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt
     8641 cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg
     8701 tgaaagattt attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca
     8761 aaattgctct ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc
     8821 cggaaaccat caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta
     8881 tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc
     8941 gtggcgtcat ctgtgagaag tgtggcgttg aagtgaccca gactaaagta cgccgtgagc
     9001 gtatgggcca catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc
     9061 cgtcccgtat cggcctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact
     9121 ttgaatccta tgtggttatc gaaggcggta tgaccaacct ggagcgtcag cagatcctga
     9181 ctgaagagca gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaaaatgg
     9241 gtgcggaagc aatccaggcc ctgctgaaga gcatggatct ggagcaagag tgcgaacagc
     9301 tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta
     9361 tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg
     9421 ttctgccggt actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg
     9481 cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac
     9541 gtctgctgga tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg
     9601 aagcggtaga cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca
     9661 agcgtcctct gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga
     9721 acctgctcgg taagcgtgtt gactactccg gtcgttctgt aatcaccgtg ggtccatacc
     9781 tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca
     9841 tctacggcaa gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg
     9901 ttgagcgcga agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg
     9961 tactgctgaa ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac
    10021 tgatcgaagg taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact
    10081 tcgatggtga ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc
    10141 gtgcgctgat gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg
    10201 ttccgtctca ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca
    10261 aaggcgaagg catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc
    10321 tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg
    10381 gtgaattagt agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga
    10441 tgattgtacc gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa
    10501 tctccaaaat gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg
    10561 cggaccagat catgtacacc ggctttgcat atgcagcgcg ttctggtgca tctgttggta
    10621 tcgatgacat ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag
    10681 ttgctgaaat tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca
    10741 aagttatcga tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc
    10801 tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca
    10861 gcatctacat gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg
    10921 ctggtatgcg tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg
    10981 cgaacttccg tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta
    11041 aaggtctggc ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg
    11101 ttgacgtggc gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca
    11161 tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtattgg
    11221 gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca
    11281 acacgctgct gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtta
    11341 aagtacgttc tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc
    11401 gtgacctggc gcgtggccac atcatcaaca agggtgaagc aatcggtgtt atcgcggcac
    11461 agtccatcgg tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg
    11521 catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca
    11581 gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg
    11641 aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagta ccttacggtg
    11701 cggtactggc aaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg
    11761 acccgcacac catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga
    11821 tcgacggcca gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg
    11881 ttctggattc cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg
    11941 ttgatgctca gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc
    12001 tgccgggtaa agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc
    12061 tggcgcgtat tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccacgcg
    12121 ttgcggacct gttcgaagca cgtcgtccga aagagccggc aatcctggct gaaatcagcg
    12181 gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag
    12241 acggtagcga tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag
    12301 gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc
    12361 tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat
    12421 accgtctgca gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc
    12481 tgcgtaaagc taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg
    12541 aatactctcg cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa
    12601 cttactcccg cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct
    12661 ccgcggcatc gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac
    12721 gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta
    12781 ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg
    12841 caccgcaggt gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc
    12901 tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc
    12961 gggttttttt atggggggag tttagggaaa gagcatttgt cagaatattt aaggaatttc
    13021 tgaatactca taatcaatgt agagatgact aatatcctga aactgactga actaattgag
    13081 tcaaactcgg caaggattcg atactattcc tgtgtagctt tcttaaggaa cgagaatgaa
    13141 acaggaagtg gagaagtggc gaccttttaa acatccggat ggcgacgttc gtgatttatc
    13201 gtttcttgat gctcatcagg ttatctacgt tcagcatcat gaggacaagg agcctttaga
    13261 gtatcgcttt tgggttacct actctcttca ctgcttcaca aaagattatg aacatcagac
    13321 gaacgaagaa aaactatcgt taatgtacca cgcgcctaaa gaatctcgtc ccttctgcca
    13381 gcaccgttat aacttagcgc gcacacactt aaaaagaact attttggcgc tgccagaaag
    13441 caacgttatt catgccgggt atggtagcta tgccgtgatt gaggtggact tagacggagg
    13501 agataaggca ttttactttg ttgtgttcag ggctttcagt gaaatttttc accattgcct
    13561 aaaacttatt gagaaataag cagcttcctc agccctcaaa ataacaaaac ccaccttaag
    13621 gtgggtttcg ccagagaatt atctctggta ttcagaacgc cattaccgga ctttgccttg
    13681 accttgcgat aatcgcaggt tgcgggatgt ctgaatttct tcagtctgct gcatcctgga
    13741 agatgagaac atgtgttctt attttcgtct ctatcatagt tgagtattta ctctcttaca
    13801 atcagatctc tttcattgct caacaggcga tggcttcaga ctttgcatta cggaattttt
    13861 aagaaaggca gggcgaaacg aggaagaagc ttttcgccct ggacaacatt cctgctgacg
    13921 gcactaccat aaaaaaacac aacgctttca acaaccttgc gtttttcatc ttccgtagcg
    13981 gctcggaata aattttcgtt cttacattcc gtctcatagt ctttgcgagg cgcgtcccag
    14041 atagctgtcc cagtctttcc ataccggctg caaaccctga gcggttaacg cggcagcaac
    14101 cgcttccggt ctgcgctcgt cgtgcggtga gaactgttcc agctcggggt gattgtcggc
    14161 atagccacct ggctgcgttt tcgaaaaagc gctgacgtta ttaattgcca gcggaataac
    14221 gcgatcgcga aaccacggtg attcccgcgt ggagagtgac agttcaatct ccggtgcaag
    14281 cagccggaag gcgcagatgg tttgcactaa ctggcgttca tccataatcg acgcaggctc
    14341 aatgccgcca gtacatggac gcaggcgtgg gaaggagaca gagtagcggc tttgccagta
    14401 atgctgttgc agccatagca aatgttctgc aaccatatag cagtcaacgc gccagctgtc
    14461 ggaaaggcca attagcgcgc cgaggcctat cttatcaatc cccgcacggc ccagccgatc
    14521 cggcgtttcc agccgccaga agaagtcctg ttttttgcct ttcagatgat ggcgggcata
    14581 agtcgcctcg tgatatgtct cctgataaac catcacgcca tccagaccaa gttgctttaa
    14641 ctcggcgtat tccgtctccg ccagcggctg tacttccatc tgtagtgaag agaactgttc
    14701 acgcagggca gggagatgac gacgaaagta atccatcccc actttcgcct gatgttcacc
    14761 ggtgactaaa agcagatgtt caaagcccat ctcccgtata gcggcgcttt ccctggcaat
    14821 atccgcttca tccagcgttt tgcgcttgat gcggttgctc atagagaatc cgcagtacgt
    14881 gcagtcgtta gcgcaaagat tggaaagata aagcgggacg tagaaactaa ctgtgttgcc
    14941 aaatcgctga cgggtcagac gctgcgcccg ttgggccagt tgttccagat agccactggc
    15001 ggctggcgat aacagcgcca tcatatcgtc gcgggtgagt tgcgaggcat ttagcgcccg
    15061 ctctacgtca gcagccgttt tgccgttgat acgcaggcgg atgtcgtccc agtccagttg
    15121 tcgccagcga tcgctgaagg ttttcatacc gatgcctcca gaaatccggt cagcgggctg
    15181 gtggcatgag caaaataact gcggctgccc ggtccggact gaggtgccag caggcctgct
    15241 tctactgcca gacgaaatgc cttcgccatg ttgacgggat cgtccgcgac ggcaatcgcc
    15301 gtattcacta acaccgcgtc ggcccccatt tccagcgcct gcgcggcatg gctgggaacg
    15361 ccgatgccag catcgacaac caccggaact gtggcctgct ggataataat ctccagcatg
    15421 gcgcgggttt ccagcccctg attcgagcca atcggcgcgc cgagcggcat caccgctgca
    15481 cagccgactt cttccagacg tttacacaat accggatcgg ccccgcagta aggcagcacg
    15541 acaaatccct gttgtaccag cgtttcggcg gctttcaggg tttcgatggg atcgggcaac
    15601 agccagcggg cgtcagggtg aatctccaat tttaaccagt ttgtgcctaa cgcttcacga
    15661 gccagatggg cggcgaaaat ggcttcttcc gctgttttcg ccccggatgt atttggcagc
    15721 agggtcacac ccgccgcgat aagcggttcg aggatagcgt cgttgtgctg gcgcaagtcg
    15781 acacgtttca tcgccagcgt caccagctgg ctgccggaag cgcggattgc ctctaccatc
    15841 agttgtgaag aggcgaattt cccggtgccg gtaaacagat gtgaatcaaa cgttttgtcc
    15901 gcaatacgta acatctcaac cccctgcaat aacctgaaaa agtaggatct ggtcgccatc
    15961 ctgcacgata tgttgcgtcc actgctcacg cgggacgatt tgctgattaa tcgccagagc
    16021 cgcgcccgct tgtcgttgaa cgagttgctc cagtagttcg tgaacagttt gcccggcggc
    16081 gcactgcatc ggttgatcgt taaacaggat ctgcattgct tcctccgcat accgggcaac
    16141 cactggcgcg gcgcaacgcc aggctgcgcc actggctcga cttaccgtcg aacagtcgga
    16201 gttctcccgc aggcgtctct ataccgctta ataacttaat ggcttccagt gcctgcaaag
    16261 tgcccataac cccgaccacc gggccaacca cgcccgccgt gcggcagttg cgttctggct
    16321 cctggttttc tggccacagg cagcggtaac aaccctgccc ccagggcggc gtcagtacca
    16381 tcaactgacc gccaaatccg accgcgctgg cggtgataag cggcgtgttg agtgccacgc
    16441 aggcggcatt aatctcctgg cgagtcgcca tattgtcggt acagtcgagc accacatcgg
    16501 cttgtgcaac cgcatctttt aacgcctcac ccgttaaccg ttgttgtaat gctgtcagtt
    16561 gaatatcggg attcaactgt gtcagtcgct gatggctgac ctgcgatttc gggcgatcga
    16621 tatcttcagt ggtaaagagg atttgtcgtt gcagattgct taaatgcaca tcgtcgtcat
    16681 ctgccagtac cagcgtcccg acgccagcgc ccgccaggta tagcgcagca ggtgtaccca
    16741 gcccgccaag accgataatc agcacctggc tgtcgagcag tttttgctgc ccgtcaagag
    16801 cgatatcgtc gagcaggatt tggcggctat aacgcataaa atcacggtca ttcatcgcca
    16861 actcctgcaa tttccagcaa ctgtgccgtt gccaaacgcc agtctgcggc ttgagtaatg
    16921 gcgctgacga cggcgatact gccgacaccc gttgctatca ccgcaggcgc gcgtgccaga
    16981 ctgataccgc caatcgccac ggtgggataa tccgacagtc gctcaacatg ccgtgccagc
    17041 tgttccagcc cctgcggtgc agaaggcatc tgtttggttt gcgtcgggaa cacatgtccc
    17101 agcgcgatat aagaggggcg tgctgccagc gcgacgtcga tttccatatc gtcatgggtc
    17161 gaaacgccca gccgcaggcc tgccgcgcgg atggcattga gatcggtggc ttgcaaatct
    17221 tcctgcccca aatggacgcc atacgcctga tgcttgatcg cgagccgcca gtaatcgttg
    17281 ataaacaatc gcgcgttata gcggcggccc agcgcaattg ccgccacgac atcggcttcc
    17341 acctcttcat cgcgccgatc tttgatgcgt agctggagag tacgtacgcc tgcatccaac
    17401 agacgttcga tccactgtac gctgtccacc accgggtaca gtcctaaacg aaaaggtaca
    17461 ggaggaaaat caggctgata catcacgctt cctccttacg caggtagatt tctccgcctc
    17521 tggcacggaa gttctccgac atatccgcta ttcccatttc aatagtttgc gtggcggcgt
    17581 aatcacgcac ttcctggctg attttcatcg agcagaattt cggcccacac atggagcaaa
    17641 aatgggcgac tttacctgac tcttgcggca gggtttcatc gtgataagcg cgggcggtaa
    17701 acgggtcgag ggccagatta aactggtctt cccagcgaaa ttcgaagcgg gctttcgaca
    17761 tggcgttatc gcgaatttgt gcgcccggat gccctttcgc caggtcagcg gcgtgggcgg
    17821 caatcttata ggtgataagc ccctgcttaa catcttcttt attaggcaga cccagatgct
    17881 cttttggcgt tacgtaacag agcatcgcgc agccaaacca gccaatcatc gccgcaccaa
    17941 tccccgacgt gaagtggtca tagcccggcg caatatcggt agttagcggc cccagagtgt
    18001 aaaacggcgc ttcgtggcag tgctctaact cctcggtcat attgcggcgg atcatctgca
    18061 tcggcacgtg gcctgggcct tcaatcatca cctgcacatc atattcccag gcgatttttg
    18121 tcagttcgcc cagcgtatgc agctcggcaa actgcgcttc atcgttggcg tcctgaatag
    18181 aaccggggcg cagaccgtcg cccagcgaca gcgaaacgtc ataagcggca cagatttcac
    18241 aaatttcgcg gaagtgttga tagaggaaat tttcctgatg atgggagagg caccatttcg
    18301 ccataatcga accgccgcgc gagacgatac cggtcaggcg tttcgcggtc atcggcacat
    18361 agcgcagcag cacccccgca tggatagtga agtaatccac accttgctcg gcctgttcca
    18421 gcagcgtgtc gcggaacgct tcccaggtaa gatcttcggc gatcccatta accttctcca
    18481 gcgcctggta gatcggcact gtaccaatcg gcaccgggct gttacgcaaa atccactcgc
    18541 gggtttcgtg aatatagcga ccggtggaga gatccatcac cgtatccgct ccccagcgcg
    18601 tggaccatac cagcttttcc acttcttctt cgatggaaga ggtgaccgcc gagttgccga
    18661 tattggcgtt aacttttacc aggaaattgc gaccaataat catcggctcc gattccggat
    18721 gattaatgtt ggccgggata atcgcacgtc cggcagcaac ttcatcacgg acaaattccg
    18781 cagtgatatt ttccggcaga cgtgcgccaa agctcattcc cggatgctgg tggcgtaaaa
    18841 cttcgctacg gatgcgctcg cggcccatat tctcgcggat ggcgatgaat tccatttccg
    18901 gtgtgatgat gccctggcgg gcgtagtgca gttgtgtgac gcggcgtcct gctttggcgc
    18961 gttttggcgt taatacgccg ctaaaacgca gctcgtcgag gccatcatct gccagccgcg
    19021 ctttagtgta atcggaactg cgcacggtaa gttcttcggt atcgccgcgc gcatcgatcc
    19081 acggctggcg tagttttgcc agcccttgct gcacgttaat ggcgatctgc ggatcgccat
    19141 acgggccgga ggtgtcgtag accggaatcg cttcgttttc ttcgtactgc ggctgttctt
    19201 tgctaccgcc aattagcgtc gggctaagct ggatctcacg catcggcacg cgcacgccgg
    19261 gttgtgtgcc agtgatataa atgcgttttg agttgggaaa ggcggtgcct tccagggtgt
    19321 cgataaaatg ttgggcctgg gcgcgttgtt cgcgacgggt cagttttgtt gcagacatag
    19381 ctcattccaa aaagttaagg acgtggcttg tcagacgacg gatgaagcaa gagacgatcg
    19441 ccgcaaaggc gatgcgatag cagattaact cttgttccct tcgcaggtat tagcctgatc
    19501 aggttccgcg gatcccgaat aaacggtctc agccagttaa ggcactccga caagaaatta
    19561 gccccacaaa cggggcgttg aatgtaaatt acgcgttaac agcgcagaac tcaagcagga
    19621 tgtttgacgc gggcggcgtc aagcaccagc agaatcagct tatcttccag cacaaagcgc
    19681 gcttccagcg cctcgccgat gtcagataaa acctgttgaa actcaaggta attatcatga
    19741 tcgatagcgg tttccaggct ggaatcgtag taatccataa tctgttgggt attggcttcg
    19801 agttgcggcc aaatcttcgc ggcgcgcgcg agttgcccgt tgccttccag cttatgaaga
    19861 atgcgctcat aaatactgaa atgtccggca gacaagtaat cgaccaggct ctgacaaaaa
    19921 tcatcaaggg ctttttcgtt tagcctcatg tacgattctt tgccaggctt aatgccaacc
    19981 agattgtagt aagccacgag cagatgctta cgtacatgta gccagcgatc aaccagtttg
    20041 ttacttcctc tgacgcgctc cgtcaggtta tcgagctggt taagcatgat tgactccgca
    20101 agtttgtatt caaaaactgc tcagtgagaa atgtaaaaac catgttaaac atgccagtga
    20161 tgcaaaggta gtgcaagagc tatggatcgt ataattgaaa aattagatca cggctggtgg
    20221 gtcgtcagcc atgaacaaaa attatggttg ccgaagggag aattgccata tggcgaagcg
    20281 gcaaatttcg atcttgtggg tcagcgcgca ctacaaatcg gcgaatggca gggggaacct
    20341 gtttggttaa tacaacagca gcggcgttac gatatggggt cggtacgtca ggtcattgat
    20401 ctcgatgttg ggctgtttca actggccgga cgtggcgtac aactggcgga gttttaccga
    20461 tcgcataaat actgtggtta ctgcgggcat gaaatgtatc caagcaaaac cgaatgggcg
    20521 atgctgtgca gccattgccg tgagcgttac tacccgcaaa tcgccccctg cattattgtt
    20581 gccatccgtc gcgatgattc gatcctcctc gcccagcata cccgccatcg taacggtgtc
    20641 catacggtac ttgccggatt cgtcgaagtg ggcgaaaccc tcgagcaggc ggtcgcgcgg
    20701 gaagtgatgg aagagagcgg aattaaagtt aaacacttgc gttatgtgac ttcccagccg
    20761 tggccgtttc ctcagtcttt aatgaccgcg tttatggcgg aatatgacag cggcgacatc
    20821 gtgatcgacc cgaaagaatt gctcgaggcg aactggtatc gctatgacga tttgccgtta
    20881 ctcccgccgc ccggcaccgt agcgcgccgt ctgatagaag atacggtggc gatgtgtcgg
    20941 gcagagtatg agtgatgata cactgaccgc ctgacgcact aaggaacagc caaaatgacc
    21001 gaacttaaaa acgatcgtta tctgcgggcg ctgctgcgcc agcccgttga tgtcactcct
    21061 gtatggatga tgcgccaggc gggtcgctat ctaccggaat ataaagccac gcgcgcccag
    21121 gcgggcgatt ttatgtcgct gtgcaaaaac gccgagctgg cgtgtgaagt gaccttgcaa
    21181 ccgctgcgtc gctacccgct ggatgcggcg atcctctttt ccgatatcct caccgtcccg
    21241 gacgcgatgg ggttagggct ctattttgaa gccggagaag gtccgcgttt tacctcgcca
    21301 gtcacctgca aagctgacgt cgataaactg ccaattccgg acccggaaga tgaactgggg
    21361 tacgtgatga acgcggtgcg taccattcgt cgcgaactga aaggcgaagt gccgctgatt
    21421 ggtttttccg gcagcccgtg gacgctggcg acctacatgg tggaaggcgg cagcagcaaa
    21481 gccttcaccg tgatcaaaaa aatgatgtat gccgatccgc aggcgctgca cgctctactc
    21541 gataaactgg cgaaaagcgt cgctttgtat ctgaatgcgc agattaaagc cggtgctcag
    21601 gctgtgatga ttttcgacac ctggggcggc gtgcttaccg ggcgcgatta tcaacagttc
    21661 tcgctttatt acatgcataa aattgttgat ggtttactgc gtgaaaacga cggtcgccgc
    21721 gtaccggtca cgctgtttac caaaggcggc ggacagtggc tggaagccat ggcagaaacc
    21781 ggttgcgatg cgctgggcct cgactggaca acggacatcg ccgatgcgcg ccgccgtgtg
    21841 ggcaataaag tcgcgttgca gggtaatatg gatccgtcga tgctgtacgc gccgcctgcc
    21901 cgcattgaag aagaagtagc gactatactt gcaggtttcg gtcacggcga aggtcatgtc
    21961 tttaaccttg gtcacggcat tcatcaggat gtgccgccag aacatgctgg cgtgttcgtg
    22021 gaggcagtgc atcgactgtc tgaacagtat caccgctaag gagcgattat ggatctcgcg
    22081 tcattacgcg ctcaacaaat cgaactggct tcttctgtga tccgcgagga tcgactcgat
    22141 aaggatccgc cggatctgat cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg
    22201 acgcgagcgg cgatggtgct gctgaaatac ccctcgcttg agctggtgga gtataaagtg
    22261 gcccgcatcg ctaccaccat gccttacatt ccaggttttc tttccttccg cgaatatcct
    22321 gcgctgctgg cagcgtggga gatgctgtca caaaagccgg atttagtgtt tgtcgatggt
    22381 catgggatct cgcatcctcg ccgtcttggc gtcgccagcc attttggttt gctggtggat
    22441 gtgccgacca ttggcgtagc gaaaaaacga ctatgcggta aattcgaacc gctctccagc
    22501 gaaccgggcg cgctggcccc actgatggat aaaggcgagc agctggcctg ggtctggcgc
    22561 agcaaagcgc gctgtaaccc gctatttatc gctactggtc atcgggtcag cgtggacagc
    22621 gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg
    22681 gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa
    22741 ttcaggtaaa ctgcggataa tttccgtatt tgagaactca acatgttaca aaacccaatt
    22801 catctgcgtc tggaacgcct ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc
    22861 gaacgcatgt accccaatta cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa
    22921 atttaccgtc gtattctcga tctcatctgg gaaacgctga ccgttaaaga tgcaaaagta
    22981 aatttcgaca gccaactgga gaaatttgaa gaagcgattc cttcagccga cgatttcgat
    23041 ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg
    23101 cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga gtaagacctc catcacgacc
    23161 gttgcgatgc tggaaatgac tcaggctggt cgcgaaatga gcgatgaaga gctcaaagaa
    23221 aacccagctg tagagcaaga atgggacatt cagtgggaaa tattccgact tttagctgag
    23281 tgcgaagaac gcgatatcga gctgataaaa ggccttaggg cagacctgcg tgaggcggat
    23341 gagagcaata ttggtataat ttttcagcaa taagaccaga aaacgtgatt taacgcctga
    23401 tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa
    23461 aaaagtggct atcggtgcgt gtatgcagga gagtgctatt ctggcatttc cgtcgcactc
    23521 gatgcttagc aagcgataaa cacattgtaa ggataactta tgaacaagac tcaactgatt
    23581 gatgtaattg cagagaaagc agaactgtcc aaaacccagg ctaaagctgc tctggagtcc
    23641 actctggctg caattactga gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc
    23701 ggtaccttca aagtgaacca ccgcgctgag cgtactggcc gcaacccgca gaccggtaaa
    23761 gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt ctggcaaggc actgaaagac
    23821 gcagttaagt aagattgcgt ggcagtgaac agttttaacg aaggggtggt ttcacccctt
    23881 ttgtctttct ggcgtcgatc attgatgctg gctggcgctc tgtttctcac tgcttgtagt
    23941 cacaactctt cacttcctcc ctttaccgcc agtggatttg ctgaagacca gggcgcggta
    24001 cgcatctggc gaaaagacag cggcgataat gtgcattttc ttgccgtgtt tagcccgtgg
    24061 cgcagtggcg ataccacgac gcgagagtat cgctggcagg gcgataacct cacgctcatc
    24121 aatatcaatg tttacagcaa acccccggtg aatattcgcg cgcgttttga cgatcgcggt
    24181 gatctgagct ttatgcaacg tgaatccgat ggggaaaagc agcagctttc taacgaccaa
    24241 atcgatttat accgttatcg ggctgatcag atccgccaga ttagcgatgc cttacgtcag
    24301 gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg aacagaccgt gaccacctgc
    24361 gaagggcaaa ccattaaacc tgatttagat tcgcaggcaa tagcgcatat cgagcgccgc
    24421 cagagccgct cttctgttga tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa
    24481 ttactgttag tggcaaactc tgatttctgt cgctggcaac ccaacgagaa aacgttctga
    24541 tttaccagtg gcccataccc atatgaccgc caccaccgca gccgccgtag cccatgccca
    24601 ttccggctcc gcgcggaata cccgcttcag ccatcgcaat atcccgtttc acccgtaact
    24661 catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt ttgctgctat
    24721 ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc agttgctgtt
    24781 gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct gtctgttgtt
    24841 cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga gcaaatgcag
    24901 atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc
    24961 gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt ccgtgccaac
    25021 gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat gactcgcctg
    25081 ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta cagcgcaggg
    25141 taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc aggcaaagaa
    25201 gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa gcgcgatcct
    25261 ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc gcgattatgg
    25321 ccgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac ttattcgtgc
    25381 tctcgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc agcagcaggc
    25441 cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca cggatgaaca
    25501 aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt attccccgca
    25561 ggaaatgcag cagttacatc cgggagatga agaagtgtgg cggcggatcg atagcgcaga
    25621 cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg ctggaatgca
    25681 ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttca tcgctttcga
    25741 cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga ttatcctctt
    25801 cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc gccgctatct
    25861 gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg tggcgctggg
    25921 gcatcttgcg gcaggcgttg cccacgaaat tcgtaacccg ctttcctcga ttaaagggct
    25981 ggcaaaatac tttgccgagc gcgcgccagc agggggagaa gcgcatcaac tggcgcaggt
    26041 gatggcgaaa gaagctgacc gtttaaaccg cgtggtaagc gagctgctgg aactggttaa
    26101 gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc actcattaca
    26161 gctggtaagc caggatgcaa actgccggga gatccagtta cgctttaccg ccaacgacac
    26221 gttgccggaa attcaggccg atccggacag gctgactcag gtcctgttga atctttatct
    26281 caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggcca gcgaaagcgg
    26341 cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag atcagcttga
    26401 agccatcttc accccgtact tcaccaccaa agccgaaggc accggactgg ggctggcggt
    26461 cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc tggagggaaa
    26521 aggcgcaagg ttcaccctct ggcttccggt caatattacg cgtaaggacc cacaaggatg
    26581 acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg cactattttg
    26641 caggctttgc tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg
    26701 ctggagcagg tgcgggagcg ggtttttgat cttgtgcttt gcgatgtgcg aatggcggag
    26761 atggacggca ttgccacgct aaaagagatc aaagcgttaa atccggcgat tccggtactg
    26821 attatgaccg catactctag cgtcgagacg gcggtagagg cgttgaaaac cggggcgctg
    26881 gattatctca tcaagccgct ggatttcgat aacctgcagg cgacgctgga aaaggcgctc
    26941 gcgcatacgc acattattga tgctgaaacg cctgcggtga ctgccagcca gttcggtatg
    27001 gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg
    27061 gaagccacgg tgctgatcca cggcgattcc ggcaccggta aagagctggt cgccagggcg
    27121 attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc
    27181 aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc
    27241 gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgttgtt tctcgatgaa
    27301 attggcgata tctcgccgat gatgctggtg cgtctgctgc gtgcgattca ggagcgcgaa
    27361 gttcagcgtg tcggtagcaa ccagactatt tcggttgatg tccggctgat tgccgcgacc
    27421 catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct ctactatcgc
    27481 ctgaacgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga tattcctctg
    27541 ctggctggcc attttctgca gcgctttgct gaacgtaatc gcaaggcggt aaaaggcttt
    27601 acgccccagg cgatggatct gttgattcat tacgactggc cgggaaatat tcgtgaactg
    27661 gaaaatgcgg tggaacgggc ggtggtgctg ctgactgggg aatatatttc cgaacgcgag
    27721 ctgccgctgg cgattgccag tacgccgatc ccgctggcac aaagcctgga tattcagccg
    27781 ctggtggaag tcgaaaaaga agtgattctg gcggcactgg agaaaacggg cggcaacaaa
    27841 accgaagccg cccgtcagtt agggatcacg cgcaaaacgc tgttggcaaa attgtcgcgt
    27901 tagttctgct cgcgttcgat agcgcgccag ccgatatctt tccggcagaa gcagtcgtcc
    27961 cagtggatat cggtcattaa ggcataggcg cgtttctgtg cttctgctac ggtatgaccc
    28021 agcgcggtga cgcacagtac gcgcccgccg ctggttacca cctgctcgtc atccgccagt
    28081 tttgtgcccg cgtggaacac tttgccgtct tccacttctt ccagcggcag gccgtggatc
    28141 acgtcaccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac gccgagagaa
    28201 gcacgttcat cccactctga cgttttctcg tctagtttgc cttcacaggc cgccaggcag
    28261 agttcaacaa gatcggactt catgcgcagc ataatcggct gggtttctgg atcgccaaag
    28321 cggcagttaa attcgataac cttcggattg ccctgtttgt cgatcatcag gcccgcgtag
    28381 agaaaaccgg tgtaggtgtt gccttccgac gccatgcctt tcacggttgg ccagatgata
    28441 cgttccatcg tgcgctgatg aacgtcatct gtcactaccg gcgcaggaga gtaagcgccc
    28501 attccgcccg tgttcggccc ggtatcttta tcgcctacgc gtttgtgatc ctggctggtg
    28561 gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc ttcgccgtcg
    28621 aggaactctt cgataacgat gcgatgaccc gcgtcgccaa acgcgttgcc cgccagcata
    28681 tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc tttcccggca
    28741 gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata cgccagcgca
    28801 ggttctacct cggtgaagtt ctgatattcc gccgtaggaa tgttatggcg ggccaggaaa
    28861 tctttagtaa acgctttcga gccttccagc tgtgcggcgc ctgcggttgg gccgaagatt
    28921 ttcatcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc ttccgggccg
    28981 acgatggtta gatctacctt ttcgttttgc gcgaaatcca gcagcgccgg gatatcggtc
    29041 acgccgatag cgacgttttg cagcgtgggt tccagcgctg tgcctgcgtt acccggagca
    29101 acaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc
    29161 ccgccgttac caatcactaa tactttcatc tgtcgctcca ttaatggcgg aagtggcgca
    29221 tatcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac
    29281 ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg gcatcaatac
    29341 cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact tccaggcctt
    29401 catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc tggcccgcgc
    29461 caataccgat ggtcatattg tttttggcat agacgatagc attggatttc acgaacttcg
    29521 ccactttcca gcagaacagc gcatcacgca gttcctgttc ggtcggctga cgtttggtga
    29581 cgacgcgcag ttcttccgca ccgaccatgc ccagatcgcg atcctgaacc agcagaccgc
    29641 cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg caggtcagaa
    29701 cgcgtacatt ctgtttggcg gcggtgattt tcagggcttc ttcgctggcg gacggcgcaa
    29761 taatcacttc aacaaactga cgagaaatga tggcctgtgc ggtttccgca tccagctcgc
    29821 ggttaaaggc aatgatgccg ccgaatgcgg aggtcgggtc ggttttgtac gcgcgatcgt
    29881 aagcatcaag aattgaattg ccgatagcca cgccgcaagg gttggcgtgc ttcacaatca
    29941 cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta tcggcgatgt
    30001 tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag gcttctttca
    30061 cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct
    30121 gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct tctttgcttt
    30181 caccgtgata agccggaacc atgctgccga agtagttggc aatcatgctg tcgtaggcgg
    30241 cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt
    30301 cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc acgattgcga
    30361 catctttatg gttcttggcg gcggagcgca ccatcgttgg gccgccgata tcgatgttct
    30421 caaccgcatc ttccagcgag caaccttcac gggccacggt ctgggcgaac ggatacaggt
    30481 taacaaccac catatcgata ggctggatct gatgttcttc cataatggca tcgtcctggc
    30541 cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg cgtccatcca
    30601 tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct ttttctgcta
    30661 acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa agtgcctggg
    30721 cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg actggacgac
    30781 gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc tgaatttttc
    30841 gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg cattgtaacg
    30901 aaaacgtttg cgcaacgctc gcgaattttt ttaattcgat ggcgatcacg attttgcccc
    30961 cgattagcgt gggagaaata gagaaataac gctgatgatt gtggataact ctgtgcgtaa
    31021 aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt ctgcaatttt
    31081 tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg cggatgtgaa
    31141 tcacttcaca caaacagccg gttcggttga agagaaaaat
//
LOCUS       DAZFDC010000047        30793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFDC010000047 DAZFDC010000000
VERSION     DAZFDC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.047"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1265)
                     /gene="espK"
                     /locus_tag="ACHJ48_002217"
     CDS             complement(<1..1265)
                     /gene="espK"
                     /locus_tag="ACHJ48_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO4707775.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLTVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNNQIMKNIKFYL"
     gene            complement(1629..2492)
                     /gene="potB"
                     /locus_tag="ACHJ48_002218"
     CDS             complement(1629..2492)
                     /gene="potB"
                     /locus_tag="ACHJ48_002218"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO4707776.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(2476..3612)
                     /gene="potA"
                     /locus_tag="ACHJ48_002219"
     CDS             complement(2476..3612)
                     /gene="potA"
                     /locus_tag="ACHJ48_002219"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO4707777.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            3862..5088
                     /gene="pepT"
                     /locus_tag="ACHJ48_002220"
     CDS             3862..5088
                     /gene="pepT"
                     /locus_tag="ACHJ48_002220"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO4707778.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(5137..6258)
                     /gene="roxA"
                     /locus_tag="ACHJ48_002221"
                     /gene_synonym="ycfD"
     CDS             complement(5137..6258)
                     /gene="roxA"
                     /locus_tag="ACHJ48_002221"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO4707779.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYTNGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            6507..7736
                     /locus_tag="ACHJ48_002222"
     CDS             6507..7736
                     /locus_tag="ACHJ48_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4707780.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            8101..8289
                     /locus_tag="ACHJ48_002223"
     CDS             8101..8289
                     /locus_tag="ACHJ48_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4707781.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            8347..8901
                     /locus_tag="ACHJ48_002224"
                     /pseudo
     CDS             8347..8901
                     /locus_tag="ACHJ48_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            9094..9291
                     /locus_tag="ACHJ48_002225"
     CDS             9094..9291
                     /locus_tag="ACHJ48_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4707782.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            9284..9496
                     /locus_tag="ACHJ48_002226"
     CDS             9284..9496
                     /locus_tag="ACHJ48_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707783.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            9486..9950
                     /locus_tag="ACHJ48_002227"
     CDS             9486..9950
                     /locus_tag="ACHJ48_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707784.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            9943..10176
                     /locus_tag="ACHJ48_002228"
     CDS             9943..10176
                     /locus_tag="ACHJ48_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707785.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            10182..10481
                     /locus_tag="ACHJ48_002229"
     CDS             10182..10481
                     /locus_tag="ACHJ48_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4707786.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            10478..11878
                     /locus_tag="ACHJ48_002230"
     CDS             10478..11878
                     /locus_tag="ACHJ48_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO4707787.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELAELRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            12079..12330
                     /locus_tag="ACHJ48_002231"
     CDS             12079..12330
                     /locus_tag="ACHJ48_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707788.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            12327..12737
                     /locus_tag="ACHJ48_002232"
     CDS             12327..12737
                     /locus_tag="ACHJ48_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4707789.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQAGFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            12748..13020
                     /locus_tag="ACHJ48_002233"
     CDS             12748..13020
                     /locus_tag="ACHJ48_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4707790.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            12977..13105
                     /locus_tag="ACHJ48_002234"
     CDS             12977..13105
                     /locus_tag="ACHJ48_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO4707791.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            13147..13371
                     /locus_tag="ACHJ48_002235"
     CDS             13147..13371
                     /locus_tag="ACHJ48_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707792.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            13623..13829
                     /locus_tag="ACHJ48_002236"
     CDS             13623..13829
                     /locus_tag="ACHJ48_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707793.1"
                     /translation="MAEEKKSGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            13829..14884
                     /locus_tag="ACHJ48_002237"
     CDS             13829..14884
                     /locus_tag="ACHJ48_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4707794.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            14897..15232
                     /locus_tag="ACHJ48_002238"
     CDS             14897..15232
                     /locus_tag="ACHJ48_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4707795.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            15245..15658
                     /locus_tag="ACHJ48_002239"
     CDS             15245..15658
                     /locus_tag="ACHJ48_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFO4707796.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPCADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            15864..16406
                     /locus_tag="ACHJ48_002240"
     CDS             15864..16406
                     /locus_tag="ACHJ48_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4707797.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            16662..16943
                     /locus_tag="ACHJ48_002241"
     CDS             16662..16943
                     /locus_tag="ACHJ48_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707798.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(17544..19004)
                     /gene="phoQ"
                     /locus_tag="ACHJ48_002242"
     CDS             complement(17544..19004)
                     /gene="phoQ"
                     /locus_tag="ACHJ48_002242"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO4707799.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMHGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITKQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(19004..19675)
                     /gene="phoP"
                     /locus_tag="ACHJ48_002243"
     CDS             complement(19004..19675)
                     /gene="phoP"
                     /locus_tag="ACHJ48_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO4707800.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLICRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(19844..21214)
                     /gene="purB"
                     /locus_tag="ACHJ48_002244"
     CDS             complement(19844..21214)
                     /gene="purB"
                     /locus_tag="ACHJ48_002244"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO4707801.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(21218..21859)
                     /gene="hflD"
                     /locus_tag="ACHJ48_002245"
     CDS             complement(21218..21859)
                     /gene="hflD"
                     /locus_tag="ACHJ48_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO4707802.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLKRKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(21895..23001)
                     /gene="mnmA"
                     /locus_tag="ACHJ48_002246"
                     /gene_synonym="trmU"
     CDS             complement(21895..23001)
                     /gene="mnmA"
                     /locus_tag="ACHJ48_002246"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO4707803.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPI"
     gene            complement(23055..23516)
                     /gene="nudJ"
                     /locus_tag="ACHJ48_002247"
     CDS             complement(23055..23516)
                     /gene="nudJ"
                     /locus_tag="ACHJ48_002247"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO4707804.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(23526..24179)
                     /gene="rluE"
                     /locus_tag="ACHJ48_002248"
     CDS             complement(23526..24179)
                     /gene="rluE"
                     /locus_tag="ACHJ48_002248"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO4707805.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATE"
     gene            24351..25601
                     /gene="icd"
                     /locus_tag="ACHJ48_002249"
     CDS             24351..25601
                     /gene="icd"
                     /locus_tag="ACHJ48_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO4707806.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYELAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(25715..26857)
                     /locus_tag="ACHJ48_002250"
     CDS             complement(25715..26857)
                     /locus_tag="ACHJ48_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4707807.1"
                     /translation="MAASPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYNSIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLSDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(26847..27083)
                     /locus_tag="ACHJ48_002251"
     CDS             complement(26847..27083)
                     /locus_tag="ACHJ48_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4707808.1"
                     /translation="MSRLITLRDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            27223..28011
                     /locus_tag="ACHJ48_002252"
                     /pseudo
     CDS             27223..28011
                     /locus_tag="ACHJ48_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            28008..28709
                     /locus_tag="ACHJ48_002253"
     CDS             28008..28709
                     /locus_tag="ACHJ48_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4707809.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            28706..29008
                     /locus_tag="ACHJ48_002254"
     CDS             28706..29008
                     /locus_tag="ACHJ48_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO4707810.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            29076..29408
                     /gene="emrE"
                     /locus_tag="ACHJ48_002255"
     CDS             29076..29408
                     /gene="emrE"
                     /locus_tag="ACHJ48_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO4707811.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAVIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            29473..29595
                     /locus_tag="ACHJ48_002256"
     CDS             29473..29595
                     /locus_tag="ACHJ48_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707812.1"
                     /translation="MFLVFVYSFVPSRLLRKQIAIRLLLYIQDSVIVIVFLNKN"
     gene            29653..>30793
                     /locus_tag="ACHJ48_002257"
     CDS             29653..>30793
                     /locus_tag="ACHJ48_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4707813.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELK"
BASE COUNT         8216 a         7071 c         7794 g         7712 t
ORIGIN      
        1 agataaaatt ttatgttttt cattatttgg ttgttgtctt tattacggta aatccatcgt
       61 aattcgctgg cggtaatact ctggccatag ccctctgact tgttagctat gagtttgagt
      121 gagttgacaa gctcatccgc acaaaaaata accgttttat tgcgtgttaa tgtctgaaac
      181 tctaggccgg ccttgcttgt ttttgcccat aatttgtcgc gattgtatac ggaaaggtcg
      241 gccatggtaa cattataatt attgtgattc tttaagtgtt gtttaaattt tattcctctt
      301 tcgtgttcat ttaatgctct tttgataagt ggatcttgct tatatttgcg aggtgttctg
      361 gggtcccata ctgcgtttgt taacgtattc tgagttataa tatctttttt ggaaaagtcg
      421 ttaactagtt taatgctgtt gacaagtttt tctctgggtg aagatagtcc atagacaatg
      481 tctccctcgc gaaagtgtaa ggccacatcc agagcgttgc cctgaaaggt gatgtttttt
      541 gctttaacta catctggaga gagtagtaca ttttgtaaat caagatccag cccatcaggc
      601 atcccttcaa caccgatgtt ccacgtggtt ttctgctccg catgaatggt taccgatgtc
      661 acactctccg gaagtttctc cggcagtata atattgctac atccagtcag cgatagcgtc
      721 ttcagtgacg gtgatatcag gttgtcaatt ctggcctgat tctccgggtt atagctattg
      781 atgctgagag atgataaccc gtttggaaca ttttttatgc tgtctgtggc gctacctttt
      841 atttcaaggt agcggacact ctctggcact ccagatatat agcagtggca aaccgtcagg
      901 cactcgatgc cttcagatat gaaatgtata aatggtgaat cagttaattc aatatgtaga
      961 ttttttacgg ttgatggcat gtctgtgatt tgtaattttt cacagttaat gacggagagg
     1021 ttttgtgtat taaccggcaa tcctgtaagg gttgtcaggt tgttgcagtt ttcgattgta
     1081 actgatgtta tttcgtttgg taactgtggc agatactcaa tatcacaatc tttgatatat
     1141 aaacgaccac tggcccgcgc ttcttctatc tgaattggtg tgatttcgct tttgatgtct
     1201 gctgatgttt cagcggaata ggagcagaat gtcccggtcg gtcgtaattg cgatgtagga
     1261 agcattgata aattccattt ttagaaataa ataacagagt aagttctatc catactacta
     1321 atcaacaagc taattttaac aaccagcaaa gccaaggtgt caatattaga aaagagagtt
     1381 atatctcaaa accaacagac tgttgatttt aacttttgcg aatactttct ccagtccgtc
     1441 atgcatggat taggattgct catttttata ccatttgttg tttttttata gcgatttgct
     1501 aaaagggaga agaaaaaacg ttctccaaaa ttagattgca actgtttgat tttatggata
     1561 gaggcatgct gtttactaga cagtaaaaag catgccaaat ttcctattaa ccttttgaat
     1621 ataaagcact aattatctat ttctgttgcc ttcttattca gcagacgaga agcgcgccag
     1681 taaaccagca acatcaggcc cattacgata gtcagcgtaa tgctggtggc tgcaccaaac
     1741 ggccagtcac gaatattgag gaactgtacc ttgatgacgt taccgatcag caggtttttc
     1801 gcaccgccca tcaggtcgga cacatagaac aggcccatcg ctggcagcat caccagcaga
     1861 catccggcaa taatccccgg catcgtcagt ggaataatga tgcggataaa agtctgtaac
     1921 ttgctggcac cgagatcgcg cgccgcctca agcaacggct tatccagttt ttcgatactg
     1981 gagtacagcg gcatcaccat aaacggtagc agaatgtaaa ccagaccgat aatcaccgca
     2041 ctgggcgtga acatgatacg aattggtgtg tcgataacgc ccagccagag caaaaactcg
     2101 ttgagatagc ctttggtgct gaggaaaatt ttcagcccgt agatgcgaat cagtgagttg
     2161 gtccagaagg gaacaatcag cagaaacagc agcagcggac gcaccttgtg tggcagcttc
     2221 gccagaaacc aggcaaacgg atagcccagc accaggcagg cgagggtggc gatcagcgcc
     2281 atattcagcg agtgcaatag cacttcaaaa tagagcggat cgagcagacg tgtgtagtta
     2341 tccagcgtaa agaccatttt gacaaaactg gcgtcgtcgc gggccaaaaa gctggtgcca
     2401 atgatcatca ggttgggcag aaagacaaac aacacaagcc aaccgacaat agtgacaatc
     2461 actacattct ggaacttact tgtgttcttc atcagccagt acgacctccc agctttctac
     2521 ccaattaatg gccatttttt ggtcgagaga gtggtcaaag tcaggatcgt cttcattgaa
     2581 gaattcgctg accatcacca tcttgccatt ttccagttca acaaccgact ccagcgtcat
     2641 gcctttgtag ttacgctcgc gaacgtaacc aatcagccct tcagcgtggt tgtcgtcgtt
     2701 aatctcttca acacgtaagt cttccgggcg cagcagaaca tgcagttttt gccccggttc
     2761 aacggcgaag ttaacgtaga tattacattc gcggccttca acgttggcgc gtacgcgctg
     2821 ctcgtcgaga cgttcgatga cagtggcgtt aaacatattg atttcgccaa tgaacccggc
     2881 aacaaacagg tttttcggct cttcgtagat ttcacgcggc gtgccgtctt gctcaatgcg
     2941 accatcgcgc atcaccacaa tcctgtctga catggtgagt gcttcttcct gatcgtgagt
     3001 cacgaagacg aatgtaatgc caagcttacg ctgtaacgct ttcagctcgt tctgcatttg
     3061 cttacgcagt ttgtaatcca gcgctgagag cgactcatcc agcaacaaca gacgaggctt
     3121 gttaaccacc gcgcgagcaa tggcgacgcg ttgctgttga ccaccagaga gctgatgcgg
     3181 tttacgttga gcgaaggttt ccagctgcac catccgcagg gcttccatca cgcggggcgt
     3241 aatttcagca gcgggggttt tttgcatgcg caacccaaag gccacatttt cgaacacggt
     3301 catgtggggg aaaagtgcgt agctttggaa aacagtgttc acatagcggt tttccgccgg
     3361 aacgtgggtg atgtcctcgt tatccaacat gatgcgtccg gaatcaacag tttccagacc
     3421 tgcaatcagg cgaagaacgg ttgttttacc gcagccagaa gggccaagca gcgtgaggaa
     3481 ctcgccattg ttgatagtca gatccagctg gggaatgacc tctttaccat caaagcattt
     3541 gcgaattccc gccaattgca ccagcggtga aagcgaactc ggttgtttat tcaatttttt
     3601 actctgtccc atgtaaacgc aacggatggc ttaccgatgc ggggtttgtg gttaaccacc
     3661 ttggtgactc ttaatgaggg cggtaattct acggcaaacc gcttgaatcg ccaatctttg
     3721 ttgtgaatta ctggcttagc tttatattca ttaaggtaat gctgataaat attcccgctt
     3781 gcaggggtaa aagtgacctg acgcaatatt tgtcttttct tgcttcttaa taatgttgtc
     3841 acaaaaagtg agggtgacta catggataaa ctacttgagc gatttttgaa ctacgtgtct
     3901 ctggataccc aatcaaaagc aggggtgaga caggttccca gcacggaagg ccaatggaag
     3961 ttattgcatc tgctgaaaga gcagctcgaa gagatggggc ttatcaatgt gaccttaagt
     4021 gagaagggca ctttgatggc gacgttaccg gctaacgtcc ctggcgatat cccggcgatt
     4081 ggctttattt ctcatgtgga tacctcaccg gattgcagcg gcaaaaatgt gaatccgcaa
     4141 attgttgaaa actatcgcgg tggcgatatt gcgctgggta tcggcgatga agttttatca
     4201 ccggttatgt tcccggtgct gcatcagcta ctgggtcaga cgctgattac caccgatggt
     4261 aaaaccttgt taggtgccga tgacaaagca ggtattgcag aaatcatgac cgcgctggcg
     4321 gtattgcaac agaaaaacat tccgcatggt gatattcgcg tcgcctttac cccggatgaa
     4381 gaagtgggca aaggggcgaa acattttgat gttgatgcct tcgatgcccg ctgggcttac
     4441 actgttgatg gtggtggcgt aggcgaactg gagtttgaaa acttcaacgc cgcatcggtc
     4501 aatatcaaaa ttgtcggtaa caatgttcat ccgggcacgg cgaaaggagt gatggtaaat
     4561 gcgctgtcgc tggcggcacg tattcatgcg gaagttccgg cggatgaaag cccggaaatg
     4621 acagaaggct atgaaggttt ctatcacctg gcgagcatga aaggcaccgt tgaacgggcc
     4681 gatatgcact acatcatccg tgatttcgac cgtaaacagt ttgaagcgcg taaacgtaaa
     4741 atgatggaga tcgccaaaaa agtgggcaaa gggttacatc ctgattgcta cattgaattg
     4801 gtgattgaag acagttacta caatatgcgc gagaaagtgg ttgagcatcc gcatattctc
     4861 gatatcgccc agcaggcgat gcgtgactgc gatattgaac cggaactgaa accgatccgc
     4921 ggcggtaccg acggcgcgca gttgtcgttt atgggattac cgtgcccgaa cctgttcact
     4981 ggcggttaca actatcatgg taagcatgag tttgtgactc tggaaggtat ggaaaaagcg
     5041 gtgcaggtga tcgtccgtat tgccgagtta acggcgcaac ggaagtaagc gaaaagggat
     5101 gcggcatgtg atgccgcatc cggcttaaat ccaaacttac ccttcgaaga accaataccc
     5161 gctattgacc agcgccgcga gcatcgcgag gaatgacgga tcttccagcg catcgccaaa
     5221 attctccgca gtcagcgcaa tgttgctggc gagtgcatcc agtgccggac ggtgcgggga
     5281 atcgatcttc tcaccattgg tatacacgtc gtcgccaatg cgcaatacgc gcagaccacc
     5341 caggcgcacc agcacatcac cttgtttcag cgcatcgtag atttcatctg gctgataagg
     5401 cggctccggc ggcgcgatat ccagttcatg acgtgactgg gatataaact cgccaaacca
     5461 ttgcttaaag tgttccggct ggttgatcaa ttcgagcatc atctcacgca gtttatccat
     5521 ctcttgcggc agaacgtccg caggatgagc gcgaggtgga acatcaggat cgctgtagta
     5581 gttgccgccc agttcgcgtt gcagcacata atcggcaaat ccgctaatta attcccgcgt
     5641 atttggcgcg cgaaaaccca cggaatagtt catcgcattt tccagcgcgt agccttcatg
     5701 cgggaatcct ggcggaatat aaagaatatc gccaggctcc agctcttcat cgatgatggc
     5761 ttcgaacgga tcgacctgta acagatccgg atgtgggcag tgctgtttca tttgcagctt
     5821 ctcgcccact cgccagcgac gacgtccggt accctgaatg ataaacacgt cgtactgatc
     5881 gagatgcggg ccgacgccgc cgccaggtac agaaaaagag atcatcagat catcaatacg
     5941 ccagtccggt agttcacgga acggtcgcat cagcgcggcg gtcggctcat gccagtggtt
     6001 cactgcttgc actaacaatg accagttagt ttcaccgaga tgatcgtagc tttcgaacgg
     6061 accgtggctg acctgccatt ttccatcctg gtgactgacc agtcgactgt cgacttcgct
     6121 ttccatcgcc agacccgcca actcgtctgg agagatcggg tcaataaaat tattaaagcc
     6181 gcgttttaac accaccgggc gtttctgcca gtgacgttca agaaaatcgg gccagttaag
     6241 agtgagttgg tattccatgt taagcatcca caggctggta tctgcaaccg attataacgg
     6301 atgcttaacg taatgcgtga agtatggaca tattttatca attagtgttt tttgccgttt
     6361 cataatgttt cagattgttc attatatttt tgattataaa cagatttgtg attttccttg
     6421 ttccagcttg tttcacgttg tagccctttg tgcaacgatc ggtgtagtta atggtgtagt
     6481 taattgtgat gaccggaatc aaaattatga gcagagcact taacaaactg agcgatacac
     6541 agctgaggaa aatcaacggc acacccgccc aaaaaacagc ctttcttaat gacggtggaa
     6601 acctgagcgt caggcattca accagtggcc ttttaacctg gtatttcact tacagggccg
     6661 gaacgggaag gggggcacca ccggaacgca ttaagctggg aaattatcct gatctgagcc
     6721 tgaaatcagc cagggaaaaa gccgcccagt gtcgcgcatg gctggcagag gggaaaaatc
     6781 cacgtcatga gcttaattac accgtacagg aagcgttaaa gccggtaacg gttggcgatg
     6841 cgctcaccta ctggcttgag tcgtacgcaa aggaaaaccg cgtggattat gccgccctga
     6901 aaaagcgcct taataatcac gtaatacagc acattggtgc tatgccgctg gataaatgcg
     6961 agctacggca ctggctggcc tgttttgacc aggtggcaaa gcgaacgcct gttactgccg
     7021 gattcttgct acagacgtgc aaacaggcgc ttaagttctg ccggaggcgg cgctatgcaa
     7081 tcagcaacgt tcttgatgat atgagtgtgg cggacgttgg gaaaaaaccg gatataagcg
     7141 agcgtgtctt aagcaccaaa gaactgggcg aattattgca ggcactggac aaaaaaatat
     7201 tctcccccta ctacatcgcg ttaatccgcc tcctgattgt gttcggatgc cggacggtag
     7261 aactgaggtt atcggagatc agcgagtggg attttaccga aatgctctgg accgttccga
     7321 aggagcacag caaaacgaag gtagccatat tccggcctat accggaagca atactgccgt
     7381 tcatcacgca gctggtggag cagaacaggc acacgggctt attgctgggg gaagtgaaac
     7441 aggaggccag cgtatcgcag tatggaagat tagcgcacag gaggcttaat caccctcact
     7501 ggtcactgca tgacatccgg cgcaccttta caaccatgct gaacgattta ggcgttgatc
     7561 cgcatgtcgt ggagcagctt acaggccacc agatgccagg aatgcagcga gtttataatc
     7621 attcccgtta tctggatgcg aaacgcaatg cgctggatat gtggacggag cggttaggga
     7681 tactggcggg aacacatgaa aacgtaacca cgctaccagt agccagaaga aaataatttt
     7741 tttcgtgttt tttcagtatg cgcatactgg atatgcgaac agatacaacg tgcaacaatg
     7801 agcaacaatg tggaacaact acgaacaaga ggcgaaaaag cgtacggatt tataagtagc
     7861 tgatttttaa tgtgttactg gttttttata cactagcgaa tcactcttta aagagcgaga
     7921 aaaaaggcat ggaacaatat tttatgcctt tgtattaaca tgaatttaaa agattttatt
     7981 cttacttttt cattttctgt aaatcataca tcctcagtat ttgccattca tcactctagc
     8041 gggaacaata cgacacaata agacacctga tgactcttta agaaacgaaa gggggcaata
     8101 gtgttaagca ctgatcggtt tatacgtgaa aaagaatgcg aaaaactaac cggccttagc
     8161 cgtacgtgtc gctaccgcct ggaaaaggcc ggacaattcc catcacgtcg taaacttggc
     8221 ggtcgttccg ttggctggtc tttatccgag gttctggcct ggaaggatag ctgcaaggca
     8281 gttcattaat cacgctggcg gcacacagcc gccacacatc aatcatctga acacagagct
     8341 ataaccatga agattgaata tacgccagaa cgtgggcggg gattcgttcg ccctggtgag
     8401 actggaaaac cacaaaattg gggtttttca ggcataaaaa aagcggcccc gaaatggagc
     8461 cgcctttctg aacagataac ccgctgcgcc ttatgtgtat atgatcccaa acataagcac
     8521 ggggatgata gccgctatca ggctggtggg caatgctatc agtctggttc agttcgttgc
     8581 catacctgca atgggcgcgt ttccctgtac tctttaagga attgctcaag ggcaaaagca
     8641 catggcgcga atctttctga ttcatgctct atctttctgc gccgtctttt ccgtgccggt
     8701 gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc aatgcgccgt
     8761 aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc gcgttgctgt
     8821 aattctttgc ggagaacgcg tttaatccaa gtggctaaag atgtgtcgcc atcggcctta
     8881 gcagcttctt ctagctgcgc tctgaagtct tccggtaatc gcatttggta tggaggcgaa
     8941 cgtttttcta atggtgttga catggtaatt accctttatt gctataccta catggtaatt
     9001 accattgtac ttgccattac acaaaaatca acgccccgca gtgctgggaa cacatacggg
     9061 gcgtctaacc aacaacgtaa actaggagcc gttatggttg ccgtaaatca tataccacac
     9121 cttgtacaca cacaaacggc ctttgtgtgg cgttttctgg cactgagtgc cggagaatct
     9181 caaatcatcc acgtaaccgc ctggacggaa cgcgaagcgc gtaaccgttg cccgtccggt
     9241 tgtgttgctg tatttgccgc ccgtattcgt caggaggtgc gccatgatta atctgtcact
     9301 tactgacctt aaccgcattc agtttcgtga gaaattcaca gggcagctac tggtcaatgt
     9361 ggataaaggt cgcgtggtgt gtaattacca cttaccagat gaagcaattg tcgccacaag
     9421 ggaatcatta caggaaatca caaaacgtgc cggaatgatt aacacgaata ttcaggggat
     9481 gctttatgca caataaaacc acaccggacg cagccgccga agccatcaaa acgctgatgc
     9541 acgcgctgat tgatatttct gttatcgcgg acagggcaca taagcacgcc accagcgaaa
     9601 cagaatatgc cggtgctttc gttcctcact cactggctgt catgcaattt agtgctgata
     9661 cggcgctgaa tgaggccaaa gctatcctga ttgctgattg tgaaaatggg ggggttatgc
     9721 gtgatgatcg ttttaattcc ctgaaacagg aattttccgg cgttcctgat gatgcggctg
     9781 atgcgctttc gtcaatgcca gaacttatta gagcggcttt tttcttactt tccacgagag
     9841 aatataaatc aacggggctt gatgtactga atatcgccgc cgattatgcg gaatatgtgg
     9901 cagaggcgcg ttatagaaga aaatttcctg aggatgtaag ccatgcgtga tatttaccac
     9961 caaacaatag accgcgcatt tcttgcactt tctcacagtg aaaacatgat ggaaatattg
    10021 cgcatatggc ttgaaacact tggcgacaat gaacgcgaca aacaaaaatc aagaattgcc
    10081 acggcattaa taacgcttct tgagcctgta ataatggaac tgcaagaaat agatctattg
    10141 cacgacagat ataaagaaca gcacaccgga gaataaaaat aatgaaactt aaatattctg
    10201 gcttaactgc cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc
    10261 agtacggcgg cacggtaatg attaagggcg tggaagaacg gcgcgtaacc taccgccgtg
    10321 aaggttacga atatgattgc gtgatgcctg tttatcagtt ccggcgtgat ttttctctgg
    10381 tacagaccgc gccgcataac gtgcccacca gcaacgccag ggcacgggca aacatccaga
    10441 agctgaaaac catggttaac ggattcaggg gcaagaaatg aaaagcgcac cgaacttaaa
    10501 aaaacagcct tacgacaaga tgaccgaagt cattattttt gcgggtagtg atgcctgggc
    10561 acatgcgaaa cagtggcagg aacaggacgg gcgactggct ggcgataatg tgcctcccgt
    10621 tgtgctggct gatgatcaac tggatgaact ggcagaactg agaatcatcg acgaggggcg
    10681 ctattgtgtc cggctgtaca aggcaggcca catcaggcca tcaaatatta atgccattgc
    10741 gcacaagctg gcggcggcgg gtgtaactga tgcgaattat taccccgaag ggatgcacag
    10801 ccatatgcgg gagaactggc gcgaatacct ggaacgggtg cgcgggaaag agccggcgga
    10861 agaaaaaaac caccagcgaa aaaccacgct accgatgagc gttggatcta ccggatacga
    10921 cacgcaactg gattacgtgg ttaaggggat tattccggcg gtatcgctat gcagcatata
    10981 cggggctagc gggtcctata aatcattcct tgccggatcg tgggcgtgcc atgttgccac
    11041 tggtcgccag tggggaggcc gcagggttgc acatggtgcg gttctctatg tggttggtga
    11101 aggcggtata ggtgttccgc gtcgtgtaaa agcctgggag gttgtgcacg atgagcaggt
    11161 gaaaaatctg tatctggtaa accgccccat ctttccggct gccccgcttg atgttgatga
    11221 aatggttatc gctgcccgtc aggtggagcg ggaaacgggt aaacctgtac gcatgattat
    11281 tctggatacg ctggcgcgtt gctttggtgg gaatgatgaa aatgattccc gtgatatggg
    11341 ggcgtttatc cgtggttgtg acgaactgaa acgacgcaca ggggccacgg tgctggtggt
    11401 tcaccattcc ggcaaggatg agacgaaagg cgcgcgcggt tccagtgcat ttcgtgcttc
    11461 gctggatgct gaataccgga tacgcaggga ggacgcagga agcgaagcgc tggttatctc
    11521 atgcaccaaa atgaaggacg cggaggaact caaagaagcc gcatatgact tacgcgtggt
    11581 ggagcttttt accgacgctg acggtgaatt aatcacgtcg ctggtggtgg tggatgatcc
    11641 gcgccctcct gttgaactgg agcgcatcga ggaggcaggg aacaagacgg aaaaccatac
    11701 cgcgctatgg gggtgcatcc gttcacgcac acagaacggc gacaagtgca cgatcccgct
    11761 gttacgtgat gacatgaaaa agctggggta tgaaatgaaa aacttccggc gctggctgta
    11821 caagctggaa aaagatgggg ttattcgtat cgatggggat gatgtagcgc cgctataaaa
    11881 gtgaggagca aaagcgaggg ggatagaaag agggccaaaa ttagcccgct ctccctcact
    11941 tttcgacctg tatacatcct caaaagtgag gggtaaaaaa atacttatga aacacacaca
    12001 tagaaaaacc gaaaatccca actgcgacga agtgagacgc ttgaaaaagt gaggcgaaaa
    12061 agtgagaggt tgcgagaaat gacccaaaaa cgcagagaca gaacagagcc aaaatataaa
    12121 gcgttagaca tgactgagca caccttaaag gtggcaatca gaacgataga ccgccacacg
    12181 cgggaaggat acgcgaagga acatcccgac ctgataagcg cattcatgac cacggcggcg
    12241 gcaaactttg ccacgctgac agaacgggaa attgccgaag cggaacaggt aacaaccatc
    12301 aacgttaaaa ccggagaggt ggaattatga cggcacagat agccgcttac gggcggctgg
    12361 tggacgaccc gcaggtaaaa cagaccagca agggcacacc gatgacgctg gcgcgtatgg
    12421 cggtatcttt gccatgcagc caggcacagg acggacaggc gacgttatgg ttatcggtca
    12481 tcgcatttgg caaacaggcc ggcttcctgg ctaaacatca aaaaggcgat gttgccagcg
    12541 tatccggcac gatgcaggta agccagtgga ccggacagaa cggagaaacg cggcagggct
    12601 ggcaggttat cgcagacagc gtaatcagtg cccgcgcggc acgtccaggc gggaacagac
    12661 gcaaaaccac aggcacacag ggtaatcagc caccagcggg aggcgatgac ccttacggtg
    12721 atgatatttc gttctgaggg ggtgacgatg gtacatgacc gcatagcgga ggaactggag
    12781 gcgaaaggct tttaccggag ggcggcggcg cgatggggtg aagtcatgca gctggtggag
    12841 acagacaagg aacggcatca catcacgatg cgacggctgg aatgttcaag gaaggcacag
    12901 agggcaccgg agccgccgga taatttcgga gacctgaaaa aggcagtcga tcgcacttat
    12961 gccgaaatgg gtatagatgg tgctggtgat gaaatatggc gcaattacca ggacagctaa
    13021 tcaaacagcc ggagaaatcc ggcttttttt gcaccagttg aaacggtatg gcgcattacc
    13081 gggttttcgt cacggtcagg catagttact atctgaaaca aacagacaca acagaggaaa
    13141 aaaacaatgc cgatgaaatt tgatgagata ttaaaacagc gtgataaata ccatgctgac
    13201 aacatggaga cgatgagcat caatgattac cgcgcattcc tggagacggg cgcactgatt
    13261 gaaaaggatc agcatggttt tgtgagatgt gctctatccg gtgaaatgct ggcggtaaat
    13321 cctgaacaga tagatgcatt gatagaattt ctgaaagaga tcagagactg agccagcaca
    13381 cagcacacat agccggagca atccggcttt tttgcgccca aaaaaagccc gataaggtca
    13441 gagggttctt atcgggcttt tgcatatgag gtttttggat gcactggcgt tcgtgatcgg
    13501 gataatcatt tcataatttg caacataact caatatcatt gcataaaatg caattctgat
    13561 tataatcagg actggataaa catccagtta tgattttttt agtcgaagag gaatttctta
    13621 ctatggctga agagaaaaaa agcggtgttt cggtgtacat aagccccgaa atcgtggagg
    13681 tgctcaagca gcgccacaaa aaaaactatg aagctggcgt tgcggctgga ctggatccgc
    13741 tgatgacgcc ggagccgtcg ataggttcac ttgtacgctc ttatttactt gcggcgcttg
    13801 ggatgcataa aaattatggg ggtgaataat ggcaggcaaa gcaacggcac ttaacactaa
    13861 ccagcttttc atgtacctga atcgcgggga tattgcggat tttaaattca gccctctgtt
    13921 taccacgctg tttttcccga acgtggcgac attcagcacg caaaacatca tgctggatac
    13981 cctggacatt gaagaagtca ccatgtcggc gttttgttcg cctatggtgg gtagccaggt
    14041 tcagcgcgat aaagggtacg aaaccagcac aatcaaacct ggctacatga agccaaagca
    14101 cgaaatcgat ccaacgaaaa ccatcatgcg catggctgga gaagatccgg cacagcttaa
    14161 cgaccctacc tatcgccgta tgcgcctgat tactggcaac atgcgccgcc agataaacgc
    14221 cattaaggca cgcgtggaat ggctggcggt gaatgcggta acgaccggaa aaaacatcat
    14281 tgagggcgaa ggcatagaac gctatgaaat cgactggaaa ataccggaaa actgcatcat
    14341 agagcaggcc aagggtaaaa aatggtccga gcaggataaa gacatgcacg acccaatcta
    14401 tgacatcgag ctttatgctg atcaggctgg ttgccccgca aacgtcatga ttatgggcgc
    14461 tgaggtatgg cgcacattac gcagctttaa aaaattccgt gagctgtacg atctttcccg
    14521 tggttcagaa tccgccgccg aactggcctg taaaaacctg ggcgaagtgg tgagctttaa
    14581 aggctatctt ggtgatctgg cccttatcgt ctattccggc aaatacactg acagcgatgg
    14641 taccgaaaaa tatttccttg agcctgattt gctggtcctg ggtaacacca acaataaagg
    14701 gctggtggcc tatggtgcga taatggatca ggaagcggta agaacgggcg caacacaaaa
    14761 catgttttac ccgaaaaact ggattgagga cggcgatccg gcgattgagt acgtgcagac
    14821 acacagtgca ccgcagccgg taccggcaga tattcgcaaa tttgttaccg tcaaaattgg
    14881 ttaacggggg gattctatgg acactccata cattgagtta tttgcaggca gtcagcaggt
    14941 atccacgacg ttggtacatt ttgccgctga tgctggcgtt attcaggaat ttaccccgct
    15001 gatgctggca gacaatggcg agtttaagcc gtgggatggt caggaatctg gcaaggctgt
    15061 ttatctgacc tcgtaccctg tggacacgtc aaagcagaaa tcagcacagt gttacaagac
    15121 ggggatattc aatattgccg ccgttaactg gcctgagagc gtcgacaccg atgcgaaaaa
    15181 atgcgccgcc tttgcgggtt ccggcgtatc cgttcagccg ctggcccgat aagcaggggg
    15241 aacgatggca acgaatgaaa gcatcatgac gctaccgctg gcgagtaaat ttaaagtcga
    15301 agcgcgggca atggctgaca gaggtttatc aacctatgag gccgtatatc aactaaacaa
    15361 actggaagag caggacaagc cgtgcgctga tgcgattatg gcgcttcatg agcataaaga
    15421 ctatcagccg ctgttgcgtg caatggcaaa cgtgccttgt atcgatgttg gtacggctaa
    15481 aagcatcctt agcatgacca tagagcagga acgtccgaag gttgcgccgg aacttaccgc
    15541 agcctttgaa aactttatgg acatgcacag cccgcaagcc gtatcagctg gcatggcata
    15601 cgatggcaga aaccagggcg atgacggcga catcgatcgc atactgaaaa ccatctgaga
    15661 caaggccgga gaaatccggc ttttttacgg gtccttcctg gaattatggc ccgttacggg
    15721 gcggcgacct cgcggatttt cgctatttat gagccttttc agggtggtgg tggtggtttt
    15781 gttgtttgat ctatctttat gaatgaaaag ggaaagatgc aagcaataca ccaacctgaa
    15841 gcagtaatta agttggtgta ttaatgaaat ctcatctgat gaacaaaaaa aacatggcga
    15901 aaagctgccg tgtaagtgcg acagcgttcg acaagtgggg agtaactccc gttgagcgta
    15961 aaggccgtga ggcgttttat gatgttgcca gcgtgataga aaatagggtt aacaatgcaa
    16021 ttagccagct tataaacgac aaaggcgaga ttgacgatga tgaactttta cgagtcagga
    16081 tcagattact gacagcacag gcagaggcgc aggaacttaa aaacgatcgc gatcgcggtg
    16141 acgtgattga tactgagttt tgcctgtacg cgctttcaaa gctggcgagt cagatttcat
    16201 caatcatgga cagcctcccg cttactatgc aaaggagctt cccacagatt acccccgcca
    16261 tgctggacag gcttaaaagg gaagtggtta aagcctgtaa tgccagtgcc agagttgccg
    16321 acaacctccc acagatactg gctgattact tgaaagaaac aaccggaaac gtaccggaaa
    16381 agttgctaca gaagaaaggc gagtaacaga cgcgcaatta ttgaacaaaa atgagaaagg
    16441 atctgataat ggcattttct ggctaaaatg cctcgtgtca gatcctttct ttttttaata
    16501 ttttcattgt taaacagtct gttacagaga agaagatcgg atctgacttt tgccggaaaa
    16561 ttctcataaa cagataaaaa ccgcgaggtc gccgccccgt aacgggccat aattccagga
    16621 aggacccgac gacaccagac tatcagagcg atgggggcac aatgacagaa gccgaactac
    16681 tgggattaat ccgccgcgtc gccggaatca gccagcagca tgacgaacag gccacacagc
    16741 cggacagcgt gaccgctgaa aattatgtgc gtgttgtagc ggaagtgatg cgccgtgatg
    16801 gtatagaact taatggcgtt gatatgcgca acatacggaa ccgcgttctt gaaatgctgg
    16861 cctacaatcg ccgcgttgaa ctgtatcggg agaaagaaaa aataacgtac cactggaaga
    16921 agccggaacg gctgcggcgg taacttgctg atattcccga taacgcaaaa ttgcgctggc
    16981 tacatcctca catctgagga ctgttaatca gaaggccgga atatattcac aaactacgaa
    17041 attttcgtag tttaattaat ggtcagatta atcgctaacc cgctgatatt ttcggaaacc
    17101 tcaatttgag gaagtcggaa gcgtcaccct tcatattctg aatggtcata atgaccacgc
    17161 agaccatcaa ggcaaccaca acgaacatcg ttacggttgg agcttttacc ccactggggg
    17221 aaaggtatta cgataatcat aacacctacc gaacaggtaa aacccaccag cctgattaac
    17281 agttaaccgg aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg
    17341 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
    17401 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
    17461 tttgctgttt ttagcacttt ttgcagtgtg ggttgtttaa ttaaatcaat gggttgcctt
    17521 gctgtggtga taaaaggaca tatttattca tctttcggcg tagaatgctg gcgaccaaaa
    17581 atcacctcca tccgcgcacc gccaagcatg ctctctccgg cgacgatttt accctcatat
    17641 tgcttggtga tttcgcgggc taccgccagc cccacacctt gcccagggcg taaagtatca
    17701 acccgttgac cacggtcgaa aatgacctct cgcttgctta atggaatacc ggggccatca
    17761 tcctcgacca caatatagag atgctcgtcg gtttgccttg cagaaatttc gacaaactcg
    17821 aggcaatatt tacaggcatt atccagtaca ttgcccatca cctcgacaaa atcgttctgc
    17881 tcaccgacaa agctgatctc tggcgaaata tcgagagaga tattgacccc tttgcgttga
    17941 tacactttgt tcagcgccga ggtgagattg tccagcagtg gggcgaccgg atgcagctcg
    18001 cggctgagca atgtcccgcc gtgcatactg gcacgatgca ggtagtagcc aatttgctgt
    18061 gaaatgcggc tgatttgctc cagcattacc ggttcagcat cactgacgct catcttttca
    18121 ctacgtagag aacgcagcgt actttgcagc accgccagtg gcgttttcag actatgggtc
    18181 aggtcggtga gcgttgtacg atatttgtcg taacgttcgc gttcactttt taacaatcgg
    18241 ttcaggtttc gtaccagact ggtcagttct cgcgttgtgg ctggattgag caattcgcgg
    18301 ttatgttctt ccagttcgcg gacttctttt gccagggctt cgatggggcg taaactccac
    18361 caggcggcga cccacagcag cgggatcact aacagcagat tggctgagag cacatagata
    18421 aaccagctcc agaccatata ggaacttttt agctccaccg gaatggtatc caccaccaca
    18481 atggttaact ttggcatccg cgatgttgcc gggtagacgt ttaccgccac cgagtgggtc
    18541 atctccgcgt cgtcatcatc ttcccgcact tcctgcaact gttgctgtat cgaatgatct
    18601 ccactcagca agaggctggt atcgttaaca tccgcttcaa tttcatgaaa accattcgat
    18661 ttcagccagt caggctggat catcttcatc agccagggca cgtcacgttg cgcccataaa
    18721 agctgcccgt tctcatcata aattagcgtc atggtggggc tttgcttgtc gatattttcg
    18781 ggtaactcga catgcaactt attgttttcc cacttcgcaa gggtatagaa cagattgctc
    18841 tcgccacgta acagccgaaa cgtagtttta tcgaaactga cgctataacc gatcagcgcg
    18901 accattccgt aggcaagcga aagcaccagt actaccgctg ccgttgccaa cagaaaacgt
    18961 acccgcagcg agagcgggaa aaaaagacgc agtaattttt tcatcagcgc aattcgaaca
    19021 aatagccctg gccgcgaacg gtagtaatca cttcttgcgg atattgtgcc tgaatttttt
    19081 tgcgcagacg tcccatcagt acatcaatgg tatggctttc ccgcagctcc gcatccggat
    19141 agagttgtag cattaacgaa tctttgctga ccactttgcc attattgcgt atcaacgttt
    19201 ccataatggt gtattcgaac gcggtcagtt tgatcacttc gtcattaata gataattcac
    19261 ggcgagagag atcaacctga aacgggggga gcgaaatgac ctgtgaagcc aggccgctat
    19321 tacgccgcat taatgcctgc attcgcgcca tcacctcttc aatatgaaac ggtttagtca
    19381 cgtaatcatc agcaccggca cttaatactt cgactttgtc ctgccagctt tcacgggcgg
    19441 ttaataccag aatcggcagt gaaacatcat tgctacgcca gcggcaaatc agtgacagac
    19501 cgtcctcgtc tggcaatccg agatcgacaa tcgcaatatc cggtaaatgt tcattgagat
    19561 aataatcggc ttctttggca tcttctgcat catcgacctg atgaccagca tcctgaatct
    19621 gaactttaag gtggtgacgt aacaacgcat tgtcttcaac aaccagtacg cgcattttta
    19681 tttctccctg tcttaattat taaaatagtg taacgcgatt atgtggttat gggggtaaac
    19741 attaaataaa ccagcggggg aggggaggta aagtgaaaaa ataaaaagcg gataatctta
    19801 ataagcaggc cggacagcat cgccatccgg cactggtacg agtttatttc agctcatcaa
    19861 ccatggtgat ggcgcgacca atgtagttag ccggcgtcat cgctttcagg cgggctttct
    19921 cttcttctgg caacgccaga ccgtcgataa actgcttcat gccttcggcg tcaacgcgct
    19981 taccgcgagt cagctctttc agcttctcgt acggtttttc gatgccatag cgacgcataa
    20041 ctgtctggat tggttctgcc agcacttccc agttgtgatc cagctcatcc agcagatggt
    20101 cacggttcac ttccagtttg ctcacgcctt tcagggtgga ttgatacgca atcagcgcat
    20161 aaccgatacc cacgccgagg ttacgcagca cggtggagtc ggtcaggtca cgctgccagc
    20221 gggaaaccgg cagtttgctt gccagatgct gcaataccgc gttggaaagg cccagattcc
    20281 cttcggagtt ttcgaagtcg atcgggttaa ctttatgcgg catggtggaa gaaccaatct
    20341 caccagcaat ggttttctgt ttgaagtggt taagggcgat ataaccccag acgtcacggt
    20401 caaagtcgat cagaatggtg ttgaagcgcg caacgcaatc aaacagttcg gcaatgtagt
    20461 cgtgcggttc aatctgggta gtgtacgggt tccattgaat acccagcgag gtgacgaact
    20521 cttcgctgaa ctgatgccag tcaacttccg ggtaagcggc gatgtgggcg ttatagttac
    20581 cgaccgcgcc gttgattttg ccgaggatct ccacctggtt aagctggcgg tactggcgct
    20641 ccatacggta ggcgacgttt gccatctctt taccgatggt tgacggcgtg gctggctgac
    20701 cgtgggtacg ggacagcagc gggatatcgc gatactgaac ggcgagatct ttaatgccat
    20761 caattagttg acgccagtac ggcaggatca cttcatcacg cgcggttttc agcattaatg
    20821 cgtgggagag gttattgata tcttccgaag tacaggcaaa gtggatgaat tcagaaaccg
    20881 cgtgcagttc cgggatctcc gccacttttt ctttcaggaa atactcaacc gctttaacgt
    20941 cgtggttagt agtacgctcg atggttttga tgcgtgcggc atcttcttcg ctgaaattgg
    21001 cgacaattgc atcaaggtaa ccgattgcgt cggcagcaaa agcaggaact tccttgatcg
    21061 ctgcgtgcgc ggccagtttt tgcagccaac gtacttcaac ttgtacacgg aatttcagca
    21121 aaccatattc gctgaaaatc ccgcgcagcg cgctgacttt atcgccgtag cgtccatcga
    21181 caggggaaac ggcggtcagt gaggataatt ccatagatca caactccggg gttaaatgag
    21241 caagaatttg ttttgcctga gtggtcaggc gattacgaga aaacatcagt tgcagacgtc
    21301 cgccgccgac ctggtgccag agcacggcgg cgcgaatgcc tgccagcagg gttgcgcgaa
    21361 ctttcgcctg cacttgtggg ctttgcagta cagcagggga accggtgacc tgaatgcgcg
    21421 ggccaagcgg gctgatcaca tcaacataga tagcagccat cgcgctcatc agcgtttcgg
    21481 actgtaaatc gaagtgttcg agctggcgtt gcaggccgtt gatccggttg cccagagtgt
    21541 cgagcgcacc tttcgctgag gagagtttgc gcttaagcac catcaggctg agtgtgtagc
    21601 gggttaattc ggcgtttaag ccctggcgac tgctggcatt gagcacgccg agcagggttt
    21661 ccagcccgac gcgcaggttg gcttcgctac cgccaaaaac cgccagcgtc gagctggggt
    21721 tcatatcaat aatactgttg agtgagacgt gtagcgcatc ggcatcacaa tgcccctggt
    21781 gagcgagttg ttgcaccagg cgtgccgact gacaaatacc ggccagggcg agggtgatgt
    21841 catagtaatt ctttgccacg ttcactgctt ccttgtttta agtaaagata ataatcagat
    21901 cggcagcggc agacgctgct caataatacc gccaccgagg cacacttcac cgttatagaa
    21961 gacggcagac tggcccggcg tcacggcggc aacgggctca tcgaaaatca cttcaatgcg
    22021 atcatcgtcc agcgccttga cggtgcaggg gatatcggtc tggcgatagc gggtttttac
    22081 cgtgcaacgc atagtaccgg tgaatggttc acgatcgacc cagtgcagct gctgggcaat
    22141 cagaccgaca gacatcagac gcgggtgttc atggccctgc gcaacaatca gaatgttgtt
    22201 ttcgacgtct ttatcgacca cataccacgg ttcttcggtg ccttctttgg tgccaccgat
    22261 acccagacct ttgcgctgac cgagggtgtg atacatcagc ccctgatgct caccaatttc
    22321 atcgccatcg actgtaatga ttttgcccgg ttgcgccggg agataacggc caaggaactc
    22381 gcggaattta cgttcgccaa tgaagcaaat gccggtagag tcttttttct tcgcggtgac
    22441 cagaccaaga tcttcagcaa tcttacgcac ctgcggtttt tccagttcgc cgaccgggaa
    22501 caggctttgc gcaatctgct catggctgag cgtataaagg aagtagctct ggtctttatt
    22561 gctgtccaga ccacgcagca ggcggctctt gccatcgaca tctgcccgac gaacgtaatg
    22621 accggtagcg atataatcgg cacctaaatc ttcggcggca aattcgagga aggctttaaa
    22681 tttgatctct ttgttgcaca gaatatccgg attcggcgtg cgaccggctt tatattcggc
    22741 aaggaacagt tcgaagacgt tgtcccagta ctcggcagca aagttaacgg tgtgcagttc
    22801 aatgccgagc ttgtcgcaga cagcctgggc atcagccaga tccgctgccg ctgtgcaata
    22861 ttcctcaccg tcgtcttctt cccagttctt cataaacagg ccttcgacct gatatccctg
    22921 ttgttgcaac agccaggcag aaacggagga atcgacaccg ccggacatgc cgacgattac
    22981 tttttttgcg gtttcagaca ttggatcact cacgacattg aacttcaagg cggcgtattc
    23041 tatcacgcag ccttttagat gacacccttt gtaaaaggcc agttaaaatc gccaatcatc
    23101 tccagcggat agcgttgccc gctttgataa caacggatac tttccgccac cagcggcgaa
    23161 cgaagatttg acgcctgtaa aatttcttcg gcgctgaccc aacgacagca gtcgatatcg
    23221 ctgtcatgag gctgggtcgg gcatatttgc tcaagctcaa tggcaaagag gaaacgcaaa
    23281 aacggcgttt tatctggcgc aatccactga tgcatacgaa taaagtgttg cggctgcgcg
    23341 ctgatgccgg tttcttccca cagctcacgg gcggctgctt ccactaaggt ttcatcggct
    23401 tccagatgcc cggcaggttg gttccataag gctttaccat taatcgtctc ttcaacgact
    23461 aaaaatttcc cctctgcgtg cactacgcaa gcaacggtaa cgtgcggttt aaacattgtg
    23521 catccttatt ctgttgcttc tcgccattcg ccattggcaa ggttatccaa agagtaatca
    23581 cccatcgcat agcgaatcag tcgcagcgtg gggaaaccaa catgggcggt catgcggcgc
    23641 acctggcgat tacgtccttc atataaggtg atcttcagcc agctggtggg aatacttttg
    23701 cgttcacgaa ttggtggatt ccgcggccat aaccacgcgg gttcgtcaac cagctccgcg
    23761 cctgcgggca gggtagggcc atcatttaag gttacgccat tgcgcaaggc ttcaagtgcg
    23821 tcttgtgtgg gaataccttc cacctgcaca taatagattt ttccggtgcg tttgcccggc
    23881 tgggttaaac gcgcctgcaa cgcgccgtta ttggtcagca ccagtaaccc ttcgctatcg
    23941 cggtcaagac gacctgctgc ataaacgccc tgaaccggga tgaattcttt taatgttttg
    24001 cgtccggctt catcggtgaa ctgcggaaga acatcgtagg gtttattgaa caggatcaca
    24061 cgcgtgggct ggttttcagg tttacgcctg gtagaacgtt gtgagctgaa tcgcttaacc
    24121 tggtgatttc taaaagaagt tttttgcatg gtattttcag agattatgaa ttgccgcatt
    24181 atagcctaat aacgcgcatc tttcatgacg gcaaacaata gggtagtatt gacaagccaa
    24241 ttacaaatca ttaacaaaaa attgctctaa agcatccgta tcgcaggacg caaacgcata
    24301 tgcaacgtgg tggcagacga gcaaaccagt agcgctcgaa ggagaggtga atggaaagta
    24361 aagtagttgt tccggcacaa ggcaagaaga tcaccctgca aaacggcaaa ctcaacgttc
    24421 ctgaaaatcc gattatccct tacattgaag gtgatggaat cggtgtagat gtaaccccag
    24481 ccatgctgaa agtggtcgac gctgcagtcg agaaagccta taaaggcgag cgtaaaatct
    24541 cctggatgga aatttacacc ggtgaaaaat ccacacaggt ttatggtcag gacgtctggc
    24601 tgcctgctga aacccttgat ctgattcgtg aatatcgcgt tgccattaaa ggcccgctga
    24661 ccactccggt tggtggcggt attcgctctc tgaacgttgc cctgcgccag gaactggatc
    24721 tctacatctg cctgcgtccg gtacgttact atcagggcac tccaagcccg gttaaacacc
    24781 ctgaactgac cgatatggtt atcttccgtg aaaactcgga agacatttat gcgggtatcg
    24841 aatggaaagc tgactctgcc gacgccgaga aagtgattaa attcctgcgt gaagagatgg
    24901 gcgtgaagaa aattcgcttc ccggaacatt gcggtatcgg tattaagccg tgttctgaag
    24961 aaggcaccaa acgtctggtt cgtgcagcga tcgaatacgc aattgctaac gatcgtgact
    25021 ctgtgactct ggtgcacaaa ggcaacatca tgaagttcac cgaaggcgcg tttaaagact
    25081 ggggctacga gctggcgcgt gaagagtttg gcggtgaact gatcgacggc ggcccgtggc
    25141 tgaaagttaa aaacccgaac accggcaaag agatcgtcat taaagacgtg attgctgatg
    25201 cattcctgca acagatcctg ctgcgtccgg ctgaatatga tgttatcgcc tgtatgaacc
    25261 tgaacggtga ctacatttct gacgccctgg cagcgcaggt tggcggtatc ggtatcgccc
    25321 ctggcgcaaa catcggtgac gaatgcgccc tgtttgaagc cacccacggt actgcgccga
    25381 aatatgccgg tcaggacaaa gttaacccag gttctattat tctctctgct gagatgatgt
    25441 tacgccatat gggttggact gaagcggctg acctgattgt taaaggtatg gaaggcgcaa
    25501 tcaatgccaa gaccgtaacc tatgacttcg aacgtctgat ggaaggcgct aaactgctga
    25561 aatgttcaga gtttggtgac gcgatcatca agaatatgta atcactacat gtgttaaata
    25621 ttgaaacggg cgtataacac gcccgttgtt ttatgatgat ttaaaatctt ccccaaaact
    25681 ttccccaaaa cccttcccca aaactggcta ttttctatgc tgttttgata tctacgataa
    25741 tccagtcttt accacgatca tcattgtatc ggtcggtcat ttttctggat ttatgaccta
    25801 acaacttttg cgtatccaga ccctgttccc gatataaccg ttctgacaga gatcgctgct
    25861 catgaaatgt gggcgcagtt ccttgctccc attttatgcc acatttttcc ctggcctttt
    25921 taaaagccgt tgtaagagta tttgcagaca cctggtctcc tctgtttgct tgagaggtag
    25981 tgtgacggta atggaccaga tatttactaa caacagcatc cctgcactga gatataactt
    26041 cacgaagggt aatattcaga gcatcgcatt tcaggttaag cggaatagca agttttgaac
    26101 cggttttttc ctgagtaatg tgcaacatgt cgtcccagat atcagagaat ttcaaattgc
    26161 agatatcgct taaacgttgt ccagtgacaa gagcaagtag catcccgcat tttaaatagg
    26221 gctgccgtct gcttacgctg tcaaatattg cctgccattc gggcagtgat aatctttgac
    26281 ggtttactcg atttcgcggt tgttttgttg cctgcgctgg gttaaatcct ggcggaacat
    26341 gtcctgcgtg ttgtgcttct ttgaagacgt cgatcaacac cattctcacg acttgcgcca
    26401 tcctgttatg accttcagcc tttacagcat caattatttc ggcaatatca agtgcggtaa
    26461 tatccttgag gtgttgcatt ccacaatgct cacggaaaag acggatgggt ttgccttttt
    26521 gccgatagga gttgggtctt agttcattat gttgcagcct gtcctcctgg atagaattat
    26581 atttatcaag ccattctgtc accgtaatgt ctgagcgcct gcctttcatt ctttccagac
    26641 gctcattgac gcttaatatt tgtcgggtac gttgttcagc aataatggta tttgcttcag
    26701 tagcaacttg ttttgcttca ttctcatcag ttcctaagct atgaaaacga ccggatagtg
    26761 gatgtttgta ttgccaatat acctttccgg ttcgcttatc taatttgcaa tataaattgg
    26821 gtatagagat tttgtgagat cgggggctag cagccatcag cgattatccg ttggagtttt
    26881 gggtttgcgt ttattgggag ttgcggttct gcaagcgttc ctacaaaacg ggaatttcgg
    26941 tcaatcatcc agtagcgacc aacttttata gcgggtgggg ccatcatttt cccttgcgcg
    27001 tattttttca gaactcgctc acttggtgct aagtccccaa attcttcttt agcccagtcc
    27061 cgtaaagtga ttagtcgaga catttgtcct cctcttagct gctgagggag tttgtgaccg
    27121 atatatctga catgatatta agctcatggc aggtacatct cttgactggt catagagata
    27181 aatttaatgc tgagaaatgc agtattgaat ttatcaattt ttcaccagct tctggtcttt
    27241 ctggctgtca tgcgcaaaac atatggcttt aataaaaaac tggattgggt tagcaacgag
    27301 caactttccg aattaaccgg gatattgccg cacaagtgtt ctgctgcaaa aagtgttctg
    27361 gtaaagcgtg ggatttttat tcagagcggg cggaataccg gcattaataa tgtggtcagt
    27421 gaatggtcaa cattacccga atcaggtaag aaaaataaag tttacctgaa agaggtaaat
    27481 ttacctgaat caggtaaaaa aagtttaccc aaatcaggta aaggcgttta cccgaatcag
    27541 gtaaacacaa aagacaaact aacaaaagac aatataaaac ctttttcgtc cgagaattct
    27601 ggcgaatcct ctgaccaacc agaaaacgat cttcctgtgg tgaaaccgga tgctgcaatt
    27661 cagagcggca gcaagtgggg gacagcagaa gacctgaccg ccgcagagtg gatgtttgac
    27721 atggtgaaga ccatcgcgcc atcagccaga aaaccgaatt ttgcagggtg ggctaacgat
    27781 atccgcctga tgcgtgaacg tgacggacgt aaccaccgcg acatgtgcgt gctgttccgc
    27841 tgggcatgcc aggacaactt ctggtccggt aacgtgctaa gtccggccaa actccgcgac
    27901 aagtggaccc agctcgaaat caaccgtaac aagcaacagg caggcatgat agccagcaaa
    27961 ccaaaactcg acctgacaaa cactgactgg atttacgggg tggatttatg aaaaacatcg
    28021 ccgcacagat ggttaacttt gaccgtgagc agatgcgtcg gatcgccaac aacatgccgg
    28081 aacagtacga cgaaaagccg caggtacagc aggtagcgca gatcatcaac ggtgtgttca
    28141 gccagttact ggcaactttc ccggcgagtc tggctaaccg tgaccagaac gaactgaacg
    28201 aaatccgccg ccagtgggtt ctggctttcc gggaaaacgg gatcaccaca atggaacagg
    28261 ttaacgcagg aatgcgcgta gcccgtcggc agaatcgacc atttctgcca tcacccgggc
    28321 agtttgttgc atggtgccgg gaagaagcat ccgttatcgc cggactgcca aacgtcagcg
    28381 agctggttga tatggtttac gagtattgcc ggaagcgtgg cctgtatccg gatgcagagt
    28441 cttatccgtg gaaatcgaac gcgcattact ggttggttac caacttgtac cagaacatgc
    28501 gggccaatgc gctgactgac gcggaattac ggcgcaaggc tgccgatgaa ctgacctgta
    28561 tgacagcgcg aattaaccgt ggtgagacga tacctgaacc agtaaaacaa cttcctgtta
    28621 tgggcggtag acctctaaat cgtgcacagg ctctggcgaa gatcgcagaa attaaagcta
    28681 agttcggact gaaaggagca agtgtatgac gggcaaagag gcaattattc attacctggg
    28741 gacgcataag agcttctgtg cacaggacgt tgccgcggta acaggtgcaa ccgtaaccag
    28801 cataaatcag gctgcggcta aaatggcgcg ggcaggaatc ctggtcgttg atggtaaggt
    28861 ctggcgaacg gtgtattacc ggttcgctac cagagaagaa cgggaaggaa aggtgagcac
    28921 gaatctgatt tttaaggagt gtcgccagag tgccgcgatg aaacgggtat tgagggtata
    28981 taaaagaaca tcaatgggta cacaatgatg aaacaggtga gttgagttca aactgtagta
    29041 caattctctc cagtttgaac aggaaagaat atgttatgaa cccttatatt tatcttggtg
    29101 gtgcaatact tgcagaggtc attggtacaa ccttaatgaa gttttcagaa ggttttacac
    29161 ggttatggcc atctgttggt acaattattt gttattgtgc atcattctgg ttattagctc
    29221 agacgctggc ttatattcct acagggattg cttatgctat ctggtcagga gtcggtattg
    29281 tcctgattag cttactatca tgggggtttt tcggccaacg gctggacctg ccagccgtta
    29341 taggcatgat gttgatttgt gccggtgtgt tggttattaa tttattgtca cgaagcacac
    29401 cacattaaaa ataatttctt ttaaaagact gcaatatggc ggttcatatc tttacatggg
    29461 ccgcttttgt taatgttttt agtttttgtg tattcttttg tgccttcaag attattgcgt
    29521 aagcaaattg caatacgatt attgttgtat attcaagata gtgtgatcgt aattgtcttt
    29581 ttaaataaaa attaaacaaa aattatatct caccactaag gtttataaaa gcataagtta
    29641 gcaggtgtca ccatgaaaaa agccatagca tatatgcgat tttcatcacc aggtcagatg
    29701 tctggcgact cattaaaccg acagagaaga cttattgctg aatggttaaa ggtaaatagt
    29761 gattattatc ttgataccat aacatatgaa gatttaggat taagtgcatt caaaggaaag
    29821 catgcacaat caggagcttt ttcggaattt ttagatgcta tagagcatgg ttatatattg
    29881 ccaggaacta cattgttagt tgaaagtctg gacagacttt caagagaaaa agtcggtgaa
    29941 gcgattgaac gtctgaaatt gattttgaat cacggtattg atgttataac tctttgcgac
    30001 aatacagtct ataatattga ctctttgaat gagccatatt cattaataaa agccatactt
    30061 atagcacaaa gggcaaatga agaaagcgag ataaagtcaa gtcgggttaa attatcatgg
    30121 aagaaaaaac ggcaggatgc actggaatca ggtacgatta tgacggcgtc ttgtccgaga
    30181 tggctctcct tagatgacaa aagaacggct tttgttccag accccgacag ggtgaaaact
    30241 attgagctaa tttttaaact caggatggaa aggcgctcat tgaatgcaat agccaagtat
    30301 ttaaatgatc atgctgtaaa gaatttctca ggaaaagaaa gtgcatgggg gccttctgta
    30361 attgaaaaat tattagcgaa taaagctctg ataggtatat gcgtaccttc atatcgtgca
    30421 agagggaaag ggataagtga aatcgctggc tattatccca gagtcatatc agatgatttg
    30481 ttttacgctg tacaggaaat tcggttggca ccttttggta ttagcaatag tagcaagaat
    30541 cctatgctaa taaatctact tcgaacagtt atgaagtgtg aggcttgtgg taataccatg
    30601 attgttcatg cggtatctgg aagtttgcat ggctattatg tttgtccgat gagaagatta
    30661 catcgatgtg acaggccatc aataaagaga gatttggttg attataatat cattaatgaa
    30721 ttgcttttta attgtagcaa aattcaacca gttgaaaaca agaaagatgc taatgaaact
    30781 ttagagttaa aaa
//
LOCUS       DAZFDC010000048        30644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFDC010000048 DAZFDC010000000
VERSION     DAZFDC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.048"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            76..210
                     /locus_tag="ACHJ48_002258"
     CDS             76..210
                     /locus_tag="ACHJ48_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707814.1"
                     /translation="MVHYEVVQYLMDCCGITYSQAVQALRSNDWDLWQAEAAICSNKM
                     "
     gene            221..376
                     /gene="ydaF"
                     /locus_tag="ACHJ48_002259"
     CDS             221..376
                     /gene="ydaF"
                     /locus_tag="ACHJ48_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4707815.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LGRHSGE"
     gene            378..740
                     /locus_tag="ACHJ48_002260"
     CDS             378..740
                     /locus_tag="ACHJ48_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707816.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTEERIIMANKSLHSAY
                     DTNTKQTDKNIIVYGHSVNREFAYFVLEKYQPTFCRIIEELTLKGKDKEFVENAVATA
                     SAAAVASIKSMITLAINQ"
     gene            complement(730..909)
                     /locus_tag="ACHJ48_002261"
     CDS             complement(730..909)
                     /locus_tag="ACHJ48_002261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707817.1"
                     /translation="MLSNMNEIDSLVFEYLRLKHPKGWDCSEADFISEVQRLRAEFTE
                     LLNPPQRDIPPGFIG"
     gene            1317..1538
                     /gene="kilR"
                     /locus_tag="ACHJ48_002262"
     CDS             1317..1538
                     /gene="kilR"
                     /locus_tag="ACHJ48_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO4707818.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            1538..1708
                     /gene="ydaE"
                     /locus_tag="ACHJ48_002263"
     CDS             1538..1708
                     /gene="ydaE"
                     /locus_tag="ACHJ48_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO4707819.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            1783..2058
                     /gene="racC"
                     /locus_tag="ACHJ48_002264"
     CDS             1783..2058
                     /gene="racC"
                     /locus_tag="ACHJ48_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HFO4707820.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            2160..4760
                     /gene="recE"
                     /locus_tag="ACHJ48_002265"
     CDS             2160..4760
                     /gene="recE"
                     /locus_tag="ACHJ48_002265"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO4707821.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCNDETGEVADPVIAGDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTTAGKEEE
                     KAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVLPT
                     FVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            4753..5562
                     /gene="recT"
                     /locus_tag="ACHJ48_002266"
     CDS             4753..5562
                     /gene="recT"
                     /locus_tag="ACHJ48_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO4707822.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            5619..5813
                     /gene="ralR"
                     /locus_tag="ACHJ48_002267"
     CDS             5619..5813
                     /gene="ralR"
                     /locus_tag="ACHJ48_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO4707823.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            5806..5994
                     /locus_tag="ACHJ48_002268"
     CDS             5806..5994
                     /locus_tag="ACHJ48_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO4707824.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSWKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            6101..6382
                     /locus_tag="ACHJ48_002269"
     CDS             6101..6382
                     /locus_tag="ACHJ48_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4707825.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFA"
     gene            6348..7424
                     /locus_tag="ACHJ48_002270"
     CDS             6348..7424
                     /locus_tag="ACHJ48_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4707826.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     IAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(7817..8158)
                     /gene="yebY"
                     /locus_tag="ACHJ48_002271"
     CDS             complement(7817..8158)
                     /gene="yebY"
                     /locus_tag="ACHJ48_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO4707827.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(8171..9043)
                     /gene="copD"
                     /locus_tag="ACHJ48_002272"
     CDS             complement(8171..9043)
                     /gene="copD"
                     /locus_tag="ACHJ48_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO4707828.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWKDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLLIISLLATLEPF"
     gene            complement(9047..9421)
                     /gene="yobA"
                     /locus_tag="ACHJ48_002273"
     CDS             complement(9047..9421)
                     /gene="yobA"
                     /locus_tag="ACHJ48_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO4707829.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKRLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9560..9790
                     /gene="holE"
                     /locus_tag="ACHJ48_002274"
     CDS             9560..9790
                     /gene="holE"
                     /locus_tag="ACHJ48_002274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO4707830.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9892..10548
                     /gene="yobB"
                     /locus_tag="ACHJ48_002275"
     CDS             9892..10548
                     /gene="yobB"
                     /locus_tag="ACHJ48_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO4707831.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            10572..11234
                     /gene="exoX"
                     /locus_tag="ACHJ48_002276"
     CDS             10572..11234
                     /gene="exoX"
                     /locus_tag="ACHJ48_002276"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO4707832.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVHPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(11231..13291)
                     /gene="ptrB"
                     /locus_tag="ACHJ48_002277"
     CDS             complement(11231..13291)
                     /gene="ptrB"
                     /locus_tag="ACHJ48_002277"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO4707833.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRNHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDLQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     RPAD"
     gene            complement(13500..14159)
                     /gene="yebE"
                     /locus_tag="ACHJ48_002278"
     CDS             complement(13500..14159)
                     /gene="yebE"
                     /locus_tag="ACHJ48_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HFO4707834.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLIGGGAVAGTVLWNKYKDKIRAAHQDEPQFGSQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(14486..14842)
                     /gene="yebF"
                     /locus_tag="ACHJ48_002279"
     CDS             complement(14486..14842)
                     /gene="yebF"
                     /locus_tag="ACHJ48_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO4707835.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(14909..15199)
                     /gene="yebG"
                     /locus_tag="ACHJ48_002280"
     CDS             complement(14909..15199)
                     /gene="yebG"
                     /locus_tag="ACHJ48_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO4707836.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            15333..16511
                     /gene="purT"
                     /locus_tag="ACHJ48_002281"
     CDS             15333..16511
                     /gene="purT"
                     /locus_tag="ACHJ48_002281"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO4707837.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYCESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(16567..17208)
                     /gene="kdgA"
                     /locus_tag="ACHJ48_002282"
                     /gene_synonym="eda"
     CDS             complement(16567..17208)
                     /gene="kdgA"
                     /locus_tag="ACHJ48_002282"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO4707838.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(17245..19056)
                     /gene="edd"
                     /locus_tag="ACHJ48_002283"
     CDS             complement(17245..19056)
                     /gene="edd"
                     /locus_tag="ACHJ48_002283"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO4707839.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(19291..20766)
                     /gene="zwf"
                     /locus_tag="ACHJ48_002284"
     CDS             complement(19291..20766)
                     /gene="zwf"
                     /locus_tag="ACHJ48_002284"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO4707840.1"
                     /translation="MAVTQTAQVCDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            21104..21973
                     /gene="hexR"
                     /locus_tag="ACHJ48_002285"
                     /gene_synonym="yebK"
     CDS             21104..21973
                     /gene="hexR"
                     /locus_tag="ACHJ48_002285"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO4707841.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            22101..23543
                     /gene="pyk"
                     /locus_tag="ACHJ48_002286"
     CDS             22101..23543
                     /gene="pyk"
                     /locus_tag="ACHJ48_002286"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO4707842.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGINYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(23674..24645)
                     /gene="lpxM"
                     /locus_tag="ACHJ48_002287"
                     /gene_synonym="msbB"
     CDS             complement(23674..24645)
                     /gene="lpxM"
                     /locus_tag="ACHJ48_002287"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310592.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO4707843.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRIAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(24765..26087)
                     /gene="mepM"
                     /locus_tag="ACHJ48_002288"
     CDS             complement(24765..26087)
                     /gene="mepM"
                     /locus_tag="ACHJ48_002288"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO4707844.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(26103..27035)
                     /gene="znuA"
                     /locus_tag="ACHJ48_002289"
     CDS             complement(26103..27035)
                     /gene="znuA"
                     /locus_tag="ACHJ48_002289"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO4707845.1"
                     /translation="MLHKKTLLFAALFAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSINGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            27114..27869
                     /gene="znuC"
                     /locus_tag="ACHJ48_002290"
     CDS             27114..27869
                     /gene="znuC"
                     /locus_tag="ACHJ48_002290"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO4707846.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            27866..28651
                     /gene="znuB"
                     /locus_tag="ACHJ48_002291"
     CDS             27866..28651
                     /gene="znuB"
                     /locus_tag="ACHJ48_002291"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO4707847.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTDGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(28897..29907)
                     /gene="ruvB"
                     /locus_tag="ACHJ48_002292"
     CDS             complement(28897..29907)
                     /gene="ruvB"
                     /locus_tag="ACHJ48_002292"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO4707848.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(29916..30527)
                     /gene="ruvA"
                     /locus_tag="ACHJ48_002293"
     CDS             complement(29916..30527)
                     /gene="ruvA"
                     /locus_tag="ACHJ48_002293"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO4707849.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         7964 a         7610 c         7504 g         7566 t
ORIGIN      
        1 aactaatatt attacagaag cagcacggcg ctgtaggttt tagttccgcc acccggcgtt
       61 aaagggagag ataaaatggt gcattacgaa gtagttcagt atttgatgga ttgttgcggt
      121 atcacttaca gccaagctgt acaggctcta cgcagcaacg actgggatct ctggcaggca
      181 gaagccgcta tatgcagcaa caagatgtga gattcgcaaa atgcaaaaaa tcgacctcgg
      241 caacaacgaa tccctagtgt gtggcgtgtt ccccaaccag gatggaacgt tcaccgccat
      301 gacgtatacc aaaagcaaaa catttaaaac tgaaacaggc gcacgtcgtt ggttgggaag
      361 acattcaggt gagtaaaatg aacgagacag aattaaaaca cgttatcgct ctgcttctgg
      421 aggatgcaaa gcgcctccag cagctggaac caaatgcagg cactgaagaa cgaattatta
      481 tggctaataa aagtctacat tctgcatatg atacaaatac aaagcaaaca gataaaaata
      541 ttattgttta tgggcacagc gttaacagag aatttgctta ctttgttctg gaaaaatatc
      601 aacctacatt ttgccggata atagaagaac taacattaaa aggcaaagat aaagagtttg
      661 ttgaaaatgc tgtggcaacg gcaagcgcag cagccgttgc cagtatcaaa tcaatgatta
      721 ctctagcgat taaccaataa acccaggggg gatatcacgc tgaggcgggt taagaagctc
      781 ggtaaactcc gctctcaggc gttgaacttc tgaaataaaa tcggcttcac tgcaatccca
      841 gcctttgggg tgcttaagtc gcaaatattc aaaaactaac gaatcaattt cattcatatt
      901 ggatagcaca attattctcc ttactggttg tgtgagaact ccagtatacc accgagcctg
      961 aagtggtaaa aagacaggcg cacaacacga aggcgcattt ccgatatcca taaagagtcg
     1021 gtcttgtctg ttaaatttaa atggtgggag tgcgcctccg gttgtaaata acgacattgc
     1081 tgtgtgtagt cctggcggca tcagtttttt tcttgaagtt cggctgatgt ccgccctttt
     1141 taaagtgaat tttgtgatgc ggtgaatgcg gctaagcgca cgtggcacag ttaaaagtca
     1201 tgttagtcct tattggtttg ggtgggaaag ccgactgtaa ttgttaactg gttgcagtca
     1261 cctggaggca ccaggcaccg catcaacaaa gttcatttgt aaaaatggag ataattatga
     1321 ttgcacatca cttcggaact gatgaaatac cacgtcagtg tgtgactcct ggcgattatg
     1381 ttcttcatga aggccggaca tatattgcct cggcaaacaa tattaaaaag cgaaaactat
     1441 atattcgtaa cctgaccaca aaaacatgca ttactgaccg catgattaaa gtcttcctcg
     1501 gtcgtgatgg tttacctgta aaggcggagt catggtgatg actaagaaaa taaaatgtgc
     1561 ttaccacctt tgcaaaaaag acgttgaaga aagcaaagct attgaaagaa tgcttcactt
     1621 catgcacggg attttatcaa aagacgaacc gagaaaatat tgcagtgaag cttgtgccga
     1681 aaaagaccag atggcacatg aactttaatt aattgactat tcgaaactga atttatgcca
     1741 gaaatggcag gtattcgctc aaccttaatt aaggagaaaa acatgattac caattatgaa
     1801 gccactgttg taactaccga tgacattgtt cacgaggtga atctggaagg aaagcgcatt
     1861 ggctacgtaa ttaaaacaga aaataaagaa accccattca ctgtggttga tatcgatggt
     1921 ccatcaggca acgtaaaaac acttgatgaa ggtgtcaaaa aaatgtgcct ggtgcatatc
     1981 ggaaagaatc tgcccgcaga aaaaaaagcc gaatttctgg caactctaat tgcaatgaaa
     2041 ttaaaaggtg aaatctgaaa gaaatagcct gcgtatggcg caggctatga acagtgtgta
     2101 tccggcaaga tcattcactg aacaaaacga attttaatct gagttgaggt taaaaaacaa
     2161 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
     2221 tcgtcctgtg ggcaagcaac gattttgaat cgacctgtgc cactctggac tacctgatcg
     2281 ttaagtcagg taaaaaactg agcagctatt ttaaagctgt tgccacgaat tttcctgtcg
     2341 ttaatgacct gcccgctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
     2401 gcaaagactc catgacatgg gaactaaaac cgggagcagc accagacaac gctcattatc
     2461 aaggcaatac caacgtcaac ggcgaagaca tgactgagat tgaggagaat atgctactcc
     2521 caatttctgg ccaggaactg cccattcgtt ggcttgctca acacggcagc gaaaaaccgg
     2581 taacgcacgt ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac
     2641 cggctgttac tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc
     2701 gcgaactcca caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa
     2761 acctgggact gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag
     2821 gactgctgac aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt
     2881 ccggtgctaa tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc
     2941 tgacgtcact ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca
     3001 tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac
     3061 cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc
     3121 gcgccatcgt ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc
     3181 acgtcactga atatctgaac aaagtactga ctgaaaccga tcatgccaac cctgatccgg
     3241 aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcagcga gtaacagaag
     3301 aaggaaaaca ggatgatgaa gaaaaaccgc aaccatctgg aacaacggca gttgaacagg
     3361 gagaggctga aacaatggaa ccggacgcaa ctgaacatca tcaggacacg cagccgctgg
     3421 atgctcagtc acaggtaaat tctgttgatg cgaaatatca ggaactgcgg gcagaactcc
     3481 atgaagcccg gaaaaacatt ccatcaaaaa atcctgtcga tgccgataaa ttgcttgctg
     3541 catcacgtgg tgaatttgtt gacggaatta gcgacccgaa cgatccgaaa tgggtaaagg
     3601 ggatccagac tcgcgattgt gtgtaccaga accagccaga aacggaaaaa accagcccag
     3661 atatgaatca acctgagcca gtagtgcaac aggaaccgga aatagcctgc aatgcctgcg
     3721 gccagactgg cggggataac tgccctgact gtggtgcggt gatgggcgac gcaacatacc
     3781 aggaaacatt cgatgaagag agtcaggttg aagctaagga aaatgatccg gaggaaatgg
     3841 aaggcgctga acatccgcac aatgagaatg ctggcagcga tccgcatcgc gattgcaatg
     3901 atgaaactgg cgaagtcgca gatcccgtaa tcgcaggaga catagagcct ggtatttatt
     3961 acggaatttc gaatgagaat taccacgcgg gtcccggtgt cagtaagtct cagctcgacg
     4021 acattgctga tactccggct ctgtatttgt ggcgtaaaaa tgccccagtg gacaccacaa
     4081 agacaaaaac gctcgattta ggaaccgctt tccactgccg tgtacttgaa ccggaagaat
     4141 tcagtaaccg ctttatcgtg gcacctgaat ttaaccgccg tacaaccgcc ggaaaagaag
     4201 aggagaaagc gtttctgatg gaatgcgcaa gcacaggaaa aacggttatc actgccgaag
     4261 aaggccggaa aattgaactc atgtatcaga gcgttatggc tttgccgctg ggtcaatggc
     4321 ttgttgaaag cgccggacac gctgaatcat caatttactg ggaagatcct gaaacaggaa
     4381 ttttgtgtcg gtgccgtccg gacaaaatta ttcctgaatt tcactggatc atggacgtga
     4441 aaaccacagc ggatattcaa cgattcaaaa cggcttatta cgactaccgc tatcacgttc
     4501 aggatgcatt ctacagtgac ggttatgaag cacagtttgg cgtgctgcca actttcgttt
     4561 ttctggttgc cagcacaact gttgaatgcg gacgttaccc ggttgagatt ttcatgatgg
     4621 gcgaagaagc aaaactggca ggccagcagg aatatcaccg caatctgagg accctggctg
     4681 actgcctgaa taccgatgaa tggccagcta ttaaaacgtt atcactgccc cgctgggcta
     4741 aggagtatgc aaatgactaa gcaaccacct atcgcaaaag ccgatctgca aaaaacccag
     4801 ggaaaccgtg caccagcagc agtaaaaaat aacgacgtga tcagctttat taatcagcca
     4861 tcaatgaaag agcaactggc agcagctctc ccacgccata tgacggctga acgaatgata
     4921 cgtatcgcca ccacagaaat tcgtaaggtt ccggcgctag gaaactgtga caccatgagt
     4981 tttgtcagtg cgatcgttca gtgttcacag ctcggccttg agccaggtag cgccctcggt
     5041 cacgcatatt tactgccttt tggtaataaa aacgaaaaga gcggtaaaaa gaacgttcag
     5101 ctaatcattg gttatcgcgg catgattgat ctggctcgcc gttctggtca aatcgccagc
     5161 ctgtcagccc gtgttgtccg tgaaggtgac gagtttagtt ttgaatttgg ccttgatgaa
     5221 aagttaatac accgcccggg agaaaacgaa gatgcaccag tgacccacgt ctatgctgtc
     5281 gcaagactga aagacggagg gactcaattt gaagttatga cgcgcaaaca gattgaactg
     5341 gtgcgcagcc agagtaaggc tggtaataac gggccatggg taactcactg ggaagaaatg
     5401 gcaaaaaaaa cggctattcg tcgcctgttt aaatacctgc ctgtctcaat tgaaatccag
     5461 cgtgcagtat caatggatga aaaggaacca ctgacaatcg atccggcaga ctcctctgta
     5521 ttaaccgggg aatacagtgt gatcgataat tcagaagaat aattcagcct ggcggtgtaa
     5581 tgcaccgcca acgtgagaca gtttttatga caaaaattat gagatatgac gatgttaaac
     5641 catgtccgtt ttgtggttgt ccatctgtta cggtgaaagc aatttcagga tattaccggg
     5701 caaaatgcaa cggatgcgaa tcccgaactg gctatggtgg aagtgaaaaa gaagcactcg
     5761 aaagatggaa taaacgaact actggaaata ataatggagg tgttcatgta taaaataact
     5821 gccactattg aaaaagaagg tggcactcct actaactgga caagatattc aaaatctaaa
     5881 ttaacgaaat cagaatgcga aaaaatgctc tcatggaaaa aagaagcagg cgtgtccaga
     5941 gagcagaaag taaagctgat aaattttaat tgcgagaaac ttctgtcctc gtgagttgca
     6001 ttatatacaa attagaactt catagctgat tattaaaaat caaccacacc cgccagtatt
     6061 ctgtatattt actggcggtc atatcgtaag aggtatggca atgaatcttg tgacactcaa
     6121 aacgtgggga aaactcagat atccggataa cccaccatca atatcaacgc tgagacgatg
     6181 ggcaaggaat ggaaacattt atcctgcacc tgaactacac gggaggagtt acagggtggt
     6241 tccggaagct ttctatatca acccaaataa ggttgatacc gatataacac accatcagcc
     6301 taatgggcga caagggagag acagtccgtt actggagaag ttaaaacatg cagcggaaaa
     6361 aatacgatcc caatttgcct aaaaacttaa catatcgaag gagggacaaa gcatattact
     6421 ggcgcaaccc tctgacgaaa gaagaattta cactaggtaa aatttcaaga agagatgcaa
     6481 tcgcgcaggc aattgaagca aatcattata tatacaaaaa ctactctcct gctgccttaa
     6541 ttgaaaaact taaagggttc gactcattta ctatggcaga ctggattgaa cgttacaaaa
     6601 cgattcttat aaggagaaaa gtgtccagaa atacttataa aattcgggta aatcaactgg
     6661 agacaataaa agaaaaattg ggagagattt tactgacaga aataaccact cgccatattg
     6721 ccgagtttct tgatttgtgg attgaaggag ggaaaaacac aatggcagga tcaatgcgtt
     6781 ctgtgttgtc tgatatgttt cgtgaggcca ttgttgaagg acgtatatct caaaatccag
     6841 taacgccaac aagagcaccg aaaatagtag ttacaagaga acgactgaaa ctaaagacat
     6901 acaactgcat cagggaggca gcagatcaac ttccggcatg gttcccatta gctatggatt
     6961 tagcccttgt aacaggacaa cgtcgcgaag acataacgaa tatgcggttt agtgatattt
     7021 atgatgatcg tctccacatc aggcaaatta agacaggaat gatgattgct atccccctgt
     7081 cactcagcct tcctgtcgct ggtctacggc ttggtacagt agttgaacag tgccgcctgg
     7141 taagccgggg agattatcta atcagtgccg ggattagaaa aaacagccct gacggcagca
     7201 ttcacccgga tggcctgaca aaaaaatttg tcgcagccag aaaattaaca ggtatccagt
     7261 tcagtgaaaa cccaccaact tttcacgaga tcagaagcct ggctggacga ttgtacaaag
     7321 aaacatgtgg agaagaattt gctcagcgtc tacttggcca cacatcggag aagacaacaa
     7381 aaatgtatct tgatgagaga gaaaaaacgt acttactgct ctgattttaa cgtaaatgga
     7441 ttgttaaatg tattttggtt gtgatataac caaaaaagac cggaatacag aaattcgagt
     7501 aaatttcggg gaatttcggg gagacgcttg caaatgattg attttaaata caattaaaaa
     7561 aagaccgagt acgattcctg tattcggtcc agggaaatgg ctcttgggag agagccgtgc
     7621 gctaaaagtt ggcattaatg caggcttagt tgccttgccc tttaagaata gatgacgacg
     7681 ccaggttttc cagtttgcgt gcaaaatggt caataaaaag cgcggtggtc atcagcttaa
     7741 atgttaaaaa ccgcccgttc tggtgaaaga actgaggcgg tttttttatt ggaaatcaaa
     7801 aggctatttt aggtaattaa cagagttttt cagctcgttc tataaacggt gccagactca
     7861 ttttttcgcc gggattgttg ggatcatcaa tctgaatcac cgaaatgggt tgggctttag
     7921 tcttcccact ggcaacttcc ttctgtgcga catcgtttaa aggatactgc acgagggtac
     7981 ttgggttgat gacatacaaa gcattacccg gtcggcaagt cagcatcacc tcttcgcgat
     8041 taaacgccca tttgtcttta cccacttcaa aacggctgac ggtaatcacc tgcggtgcag
     8101 ccagcgccgc tgcagaactg gtgagtaaca gaaacgccag aatacttttt ttcatcattt
     8161 tcgtaatcca tcaaaaaggt tcgagagtcg caagcaggct gatgatcagc agcactaccg
     8221 cacctatcgc ccattcgagt ttagtcatcc acacaaacca gggtgccgca cgatcttcat
     8281 cctgcctcat gcgcggtaca agaacataac gattagccag cgcaatgacc accataatca
     8341 tcaccaggat ggctttgagc aataagagtt ggccccagta agtcgtaagc gtggggggaa
     8401 atccggtaat caacaatgca ttaagcacgc cgcttgccag tacgccgatc accgcaaaat
     8461 gcccgcacca ggaaaaacgc atcagcgcct gaatagcctg gtgtcgccaa cggcctttga
     8521 tgagttgcat acaccagagc accggcagta atcccccaaa ccacgccgcc gcacaaatca
     8581 ggtgaatcgc gtgattagtc tgatggattt tcgccgttac cccttcattc agcgtcgcat
     8641 gccccactcc cgccagcaga ataaattgcg cggtagtgag cataaacagc aaacgtggca
     8701 tattccgcgg ttgcataagg gcgacgatca acgttacgag ggcgagaaca atttgccata
     8761 accagacgcc accaaactgc gtttgcaaaa cagcctgcca aatgtttggc gaaaatacat
     8821 ctttccatcc cgttcccatc aacccgccct gaacggcgag cattgcagtg gcactgatca
     8881 aactccagac ggcagcgtgc tgttgtaaac gcagaaaacg cttcgtcagc aagcgacgaa
     8941 tcgttaaggg tgccagccag gcaccgtaca ttgcgaagcc aaagaccagc atcagcgagg
     9001 tgaaatggat aaatcgcagc gcgatccagg taaacgccag catgatttat ttcacgctaa
     9061 aggtgtagtg tcctttcgtt ttatgcccgt ccaccgaaac aacatgccag tcaacggtgt
     9121 aggttccggg tttcagcgaa tcggccagtg gaacaatcag tcgcttttgg tcctgctcat
     9181 ttcgcttcgc cggtaatgtt ttaatatttt cgttttttgg ccccgtgatt tttgcaccac
     9241 tgaatcctgt ttcaacacct tccgagaagt ttaaagtgat tgcctgcggt gcagctgtca
     9301 cttgcgcgtt tgccgcagga tactgatgcg ttaaatgcgc atgtgcccag acggaaggtg
     9361 tcaccaacga agtggtcagg attgccagcg cgtagcgaag ggagcgtgca gttgaagcca
     9421 tattatctat tcctttttgt aataactttt ttacagagca taaccttgtc taatgtctga
     9481 gtcgaggatc atcaattccg gcttgccatc caggctcact cttagtaact tttgcccgcg
     9541 aatgatgagg agattaagaa tgctgaagaa tctggctaaa ctggatcaaa cagaaatgga
     9601 taaagtgaat gtcgatttgg cggcggccgg ggtggcattt aaagaacgct acaatatgcc
     9661 agtgatcgct gaagcggttg aacgtgaaca gcctgaacat ttgcgcagct ggtttcgcga
     9721 gcggcttatt gcccaccgtt tggcttcggt caatctgtca cgtttacctt acgagcccaa
     9781 acttaaataa aacttataca gagttacact ttcttacata acgcctgcta aattatgagt
     9841 attttctaaa ccgcacccat aatttgcagt cattttgaaa aggaagtcat tatgtcgttc
     9901 tggaaagttg cagcagcgca atatgagcct cgtaaaacct cactcaccga acaggtagct
     9961 catcatctgg agtttgtgag ggccgccgcc aggcaacaat gtcagctgtt agtttttcca
    10021 tcgctttctt tactggggtg tgattattcg cgacgtgccc ttcctgcccc acccgatctc
    10081 tcactgttag acccgctttg ttatgccgca acgacctggc gaatgaccat cattgccggc
    10141 cttcccgttg aatataacga tcgctttatt cgtggaattg cggtgttcgc accgtggcga
    10201 aaaacgcctg ggatctacca tcagagtcat ggtgcatgtc tgggccgtcg ttccagaacc
    10261 atcacggtgg tcgatgaaca accgcaaggt atggatatgg acccgacctg ttcactgttt
    10321 acgacagggc aatgtctggg agaacccgac ctgctggcat cggcccgtcg cttacaattt
    10381 ttttcacatc agtactcgat tgctgtgctg atggcaaatg cccgtggtaa cagtgcacta
    10441 tgggatgaac atggtcgtct catcgttcgc gccgatcgtg gttcattatt gttagtcggt
    10501 cagcgttcat cacagggttg gcaaggcgat atcattccat tacgctaggc tttttcggcc
    10561 tggagcatgc catgttgcgc attatcgata cagaaacctg cggtttgcag ggagggatcg
    10621 ttgagattgc ctctgttgat gtcattgacg ggaaaatcgt caaccccatg agccacctgg
    10681 tgcaccccga tcgtcctatt agtccacaag cgatggcgat tcatcgcatc accgaagcca
    10741 tggtcgccga taaaccgtgg attgaagatg tgatcccaca ctattacggt agtgaatggt
    10801 atgtcgcgca taacgccagc tttgaccgcc gcgtactgcc tgagatgccc ggtgagtgga
    10861 tttgcactat gaaactggcc cgtcgtttgt ggccagggat caagtacagc aatatggcgt
    10921 tatataaaac acggaagctc aatgtacaga cgccgccggg cctgcatcat caccgcgcgt
    10981 tgtatgactg ttatatcacc gccgcgttgc ttatcgatat tatgaacacc tccggctgga
    11041 cggcagaaca gatggccgat ataaccggac gtccgtcgtt gatgacgacc ttcacctttg
    11101 gcaaataccg tggcaaagcg gtttccgacg ttgccgaacg cgatccgggc tatctgcgct
    11161 ggttgtttaa taacctggac agcatgagcc cggagctgcg tttaacactg aaacattatc
    11221 tggaaaatac ctaatccgca ggcctagcgg gtaatgttcc ctgcgccagc gcgaccagaa
    11281 aagcatattc catcgctacg ccttcgtacg atttaaagcg accagattta ccgccatggc
    11341 ctgagtccat gtcggtacag agcaataaaa gatggttatc ggttttcagc tcgcgcaact
    11401 tagcgaccca ttttgccggt tcccaatatt gcacctgaga atcgtgtaaa ccggtcgtta
    11461 ccagtaaatg tggataagcc tgtgcggtga cgttgtcata cgggctgtag cttttcatat
    11521 actcgtaata ttgcagatcc tgcggattcc cccactcttc aaactcacca gtggtaagag
    11581 gaattgattc atcaagcatc gttgttacaa catcaacaaa cggtacctgg gcgataacgc
    11641 cgtggaataa ttccgggcgt tgattaattg caacgcccat caacatgccc cccgcactcc
    11701 cgcccatcgc ataacaaagc gaaggagagc catagcccag ttttaacaat gcatcgcagg
    11761 catcaagata atcattaaac gtatttttct tcttcagaaa tttaccgtct tcgtaccatt
    11821 gttgccccag ctcaccgccg ccgcgaacat ggacaatggc gtagacaaag ccacgatcta
    11881 acaaactcaa gcggctaaaa ctgaaatcgg catcaatact tgcgccgtaa gaaccgtagc
    11941 catacaccag cagcgggttg tgtcctttgc gaaaatgatt gcgatggtag accaacgaaa
    12001 ccggaacttc gacaccatca cgggcgacta tccacaggtg ttcactgcgg taattcgccg
    12061 catcaaaacc aggaacttct gtttgtttta atacacgacg ctcaccggta tccatatcca
    12121 gttcaaacaa agtgtctggt gtggtcatgg aagaataacc ataacgcaat cgcgcggttt
    12181 caggttctgg attgtaggca atccaggtca cataggctgg atcatcaaag gcaataccaa
    12241 tgacttcccg ggtcttgcgg ttaatttggc gcaaactggt taacccgcgc tgacgctctt
    12301 caaccaccag ccagtcggta aacagcgtaa acccttccag catgatgttt tcgcgtggcg
    12361 gaattaactc ttcccactgt tgctcatcac gcatacgggt acggtataag ccaaagtttt
    12421 tgccgtggcg gttggaacgc agataaaaac gatgctggta gtgatcaagg ctgtattcgt
    12481 gatctttgcg gcgcggcaga aaaacaaacg gctcggcatc tgccatttcc gcgtccagca
    12541 ggcgaacttc actggtggtg gcgctggcca aatgaatgac tacatagtgc tttgaggtcg
    12601 ttttatgcag gctgacgtaa taggtatcgt ctttttcttc gtaaatcagt ttatcttgcg
    12661 atgctggcgt accgatggcg tgacgccaga cctgataagg cagcagcgtc accggatgct
    12721 tgcgaacata gtagaaagtc caggagtcat ttgcccagac aaagctgggt tcaacgttat
    12781 ccagcagttc cgggtaccag ttacctgttt ccagattacg aaaacgaatg ccgtactggc
    12841 gtcgggaaag aaaatcttct gccagcgcca taatggtgtt atcgggcgta atcgccattc
    12901 cgcccatcga ataaaactca ctatgagccg cgcgcttgtt ggcatcgagc aatatttccc
    12961 actcgtccca ctcttcactg aacgccgatt gacgctggta gatagcatat tcacagcctg
    13021 gttcataaat atgccggtag cggtagccat ttttgatgta gggcgcagaa acttctcgtt
    13081 gcggaatgcg gtcgatgatt tcctttaaga tgcgatcctg caaggcttgt tgtgaggcca
    13141 tcacccgatg accgtaacta ttttcttgtt gcaggtagtc caggacttcc ggctgagaac
    13201 gcgtatcgtc ccgcagccag tagtaattat cgatgcgcgt atcgccatga agcgtcatgg
    13261 cgtggggaat gcgggcggct tttggtagca tgttattgtt ctttctggtt gaaacatctt
    13321 ataagggtgg caaaactcac cggggatgca agcgaaacag ggcagtcatt gcttagatga
    13381 tgacaggtaa tggcgcgaat atcgaatgtt atgcagacag agaaaatcag cctgttcaaa
    13441 tggctgtgct gttctggata gcccgaaata gtcaacttca ggctatccag agagcggaat
    13501 tattccgcca aagtgcgttt ttgctgttcg agatcgcgtt caatgccttc acgaacatcc
    13561 tgggggattt tcagcgcgtc acccagtgca ttcaggtaac tgcgttccat aaaatggtca
    13621 atatcaatag ccgcgcagct caggaaatag atttccagcg cctcttcttc attgcggacc
    13681 ccggtagcca ggcgttgtgg atccagcggt tgttcgattg cctgctcaat gagtacccgc
    13741 ccctgctctt ccacgccggc ttcacgcaat tgctggtcga ttgccgcacg ttctttggca
    13801 tcaatatgac catcactttt agcggcaaag actagcgcaa ggatcaaacg ttccgtacgc
    13861 tcatccagcg gcgtactttg cgagccaaac tgcggttcgt cctgatgcgc cgcgcgaatt
    13921 ttatctttgt atttattcca cagcaccgta cccgctaccg ctccgccgcc aatcagcaac
    13981 gcgttggtgc catatttcgt aagaagttta cgtgctgatt tatttgcgac cagcagtcca
    14041 gccagtccgc ctaatgcgcc cggcactaaa agtttgccca atccctgatc cgcagacgag
    14101 gaggtagaag aactgctttg cccaagcagg gattgcagtt gatttaacca gttagccata
    14161 tttgctcctc aataaccatt tttagtgcct gtagcataga ggatgaggat gtcagaaaat
    14221 gtatatcgag acaaaagatg cgcaaataag cggggcggta ggccgttatt caaagaaatt
    14281 cgcgccaggt aaggtattta ctgtatatca ggtgataagg gtattttagg tgaaagtagt
    14341 ttgtcgtttt gagtggcatt gtttgatgct gattgcccga tgcgacgctg ttgcgtcttc
    14401 tcaggcctac gattgtggtg cgttctgggg cggacaaggc actcgcgccg catccgccaa
    14461 cggtcaatcg aattatcaga acccattaac gccgctgata ttccgccatt cccgctttgc
    14521 agtccacgct gacctggtaa tgaatatcgg cacttttacc acgtacggtt agcggtactg
    14581 accatttatc atctttaccc tgaatgtcct gcaaactgac ccatgccacg ggatcggcct
    14641 gaccgacaat tttttgatca tctgcccagc gcgccacgcg attttgttga taatcacgtt
    14701 ttacgctcgc ggcaattccg gcagcatcca gaccttcaca ctttgggaaa gtgaccgact
    14761 tgctggtttc attattggca gcgaaaactg atgcgcaggc agaaaccaac aacagcccta
    14821 aaaacgcccc tctttttttc atgtttttct ccatagcaca atgattcagg agaaagcatg
    14881 gtacaaattg tcaggagcgc aagttgcttc aggccgcgtg tgaggcatct tcctcttcgg
    14941 attcagcacc gacgacctgt ggggacggta atttgccggt tttcagaatg gtgctcagca
    15001 catctttttg ttctgccagc catagcgaaa gggcttcacg ttgctcgtct tccatttgca
    15061 ctggagaatt tgtcagccag gtgtcaagca gatccgccgt atcaagcatt ttgtcataag
    15121 catcggcttc ctttttgctg gtaaacgaca ttttctcttc gccctcacga atgactacgt
    15181 atttaacttc aaccgccatt tgcagcctct cataataact gtgattctat acagtatatt
    15241 tcttttcggt tgagagatca acaccagcaa taaagacaca cgcaaacgtt ttcgtttata
    15301 ctgcgcgcgg aattaatcag gggatattcg ttatgacgtt attaggcact gcgctgcgtc
    15361 cggcagcaac tcgcgtgatg ttattaggct ccggtgaact gggtaaagaa gtggcaatcg
    15421 agtgtcagcg tctcggcgta gaggtgattg ccgtcgatcg ctatgccgac gcaccagcca
    15481 tgcatgtcgc gcatcgctcc catgtcatta atatgcttga tggtgatgca ttacgccgtg
    15541 tggttgaact ggaaaaacca cattatatcg tgccggagat cgaagctatt gccaccgata
    15601 tgctgatcca acttgaagag gaaggactga atgttgtccc ctgcgctcgc gcaacgaaat
    15661 taacgatgaa tcgcgagggt atccgtcgcc tggcggcaga agagctgcag ctgcccactt
    15721 ccacttatcg ttttgccgat agcgaaagcc ttttccgcga ggcggttgcc gccattggct
    15781 atccctgcat tgtaaaaccg gtgatgagct cttccggcaa ggggcagacg tttattcgct
    15841 ctgcagagca acttgctcag gcatgggaat acgctcagca aggcggtcgc gccggagcgg
    15901 gccgcgtaat tgttgaaggc gtcgttaagt ttgacttcga aattaccctg ctaaccgtca
    15961 gcgcggtgga tggcgtccat ttctgtgcac cagtaggtca tcgccaggaa gatggcgact
    16021 actgtgaatc ctggcaacca cagcaaatga gcccgcttgc ccttgaacgt gcgcaggaga
    16081 ttgcccgtaa agtggtgctg gcactgggcg gttatggtct gtttggtgtc gagctgtttg
    16141 tctgtggtga tgaggtgatt ttcagtgagg tctcccctcg tccacatgat accgggatgg
    16201 tgacgttaat ttctcaagat ctctcagagt ttgccctgca tgtacgtgcc ttcctcggac
    16261 ttccggttgg cgggatccgt cagtatggtc ctgcagcttc tgccgttatt ctgccacaac
    16321 tgaccagtca gaatgtcacg tttgataatg tgcagaatgc cgtaggcgca gatttgcaga
    16381 ttcgtttatt tggtaagccg gaaattgatg gcagccgtcg tctgggggtg gcactggcta
    16441 ctgcagagag tgttgttgac gccattgaac gcgcgaagca cgccgccgga caggtaaaag
    16501 tacagggtta aacccggcaa ataaacgcta caaaaatgcc cgatcctcga tcgggcattt
    16561 tgacttttac agcttagcgc cttctacagc ttcacgcgcc agcttagtaa tgcggtcgta
    16621 atcgcccgct tccagcgcat ctgccggaac cagccaggaa ccaccgatgc acagcacgct
    16681 tttcagcgcc aggtagtcac ggtagttagc cggagaaata ccacccgtcg ggcagaaacg
    16741 gacctgggag aacggacccg cgatcgcctg cagggctttc acgccgccgt tagcttcagc
    16801 cgggaagaat ttgaactctt tcaaaccgta gtccataccc agcatcagtt cggaaacagt
    16861 gctgatcccc ggaatcagcg gaatagtccc ttcggtagca gctttcagca gcggctcggt
    16921 cagacccggg ctaattgcga actgtgcacc cgcttcagtg acttctgcca gctgctgtgg
    16981 attcagcacc gtaccggcac ccacaatcgc ttcaggcact tctttggcga tagcacggat
    17041 agcgtcaact gcacactcgg tacgcagagt cacttccaga acgcgcaccc caccagcaac
    17101 caacgctttt gccatcggca ccgcgtgttc cagttttttt accacgataa ccggtacaac
    17161 cgggccggtg gtcaggattg attctgcact tgttttccag tttttcatca gagttttctc
    17221 tcgcctgatt acaaatttgt cgtcttaaaa agtgatacag gttgcgccct gttcggcacc
    17281 ggacagtttt tcacgcaagg cgctgaataa ttcacgtcct gttcccacgc gtgacgcgct
    17341 caggtcagga atgtgtggtt cgcgagcagc caattccgct tcgtctacca gcagcgtcag
    17401 ttcgcctgtc tgtccattca cacgaatgat gtccccgtcg cgcacttttg ccagcagccc
    17461 gccatcgtaa gcttctggtg ttacgtggat agctgacggc actttacctg aagcgccgga
    17521 gagtcgtcca tcggtaacta acgcaatttt gaaacaccgg tccaataata caccaagtgg
    17581 cggcatgagt ttatgtaatt ctggcattcc gttcgctttt ggcccctgat gacggacaac
    17641 aacgacacaa tcgcggtcca gcaaacccgc ttcaaaggcc ggcataacgt catgctggct
    17701 ttcaaaaaca accgctggcg cttcaatcac ctggttctca accggcacgg cagaggtttt
    17761 cataaccgca cggcccaggt taccgcttaa cacttttgtc ccaccatgat gagagaaagg
    17821 ttgttcgaag gaagcgatca cattgctgtc gagtgatttt tccgcccctt cccgccagtc
    17881 cagttcacca ttattcagcc atggttcaag ggtataacga gacagaccaa agcctgccac
    17941 cgtattgaca tcttcatgca gcaggcctgc tttgagcagt tcacgcacca gaaccggtac
    18001 gccacctgcc gcctggaagt ggttaatatc ggccggaccg ttcgggtaga gacgtgccat
    18061 cagcggtaca acatcagaaa ggtcagagaa gtcatcccag ttaatctgaa taccggccgc
    18121 gcgcgccatc gccaccaggt gcatggtgtg gttagtggaa ccaccggtcg ccagcagtgc
    18181 aacgataccg ttcaccacca ctttctcatc gatcatctta ccgatcggca tccattcatt
    18241 accattaccg gtcatgcgtg taacctgacg cgcagcggca gcggtcaaag catcgcgcag
    18301 cggagaatcc ggatgaacaa aagaagagcc tggcaactgc atccccataa actccaccac
    18361 catctggttg gtgttggcag taccgtagaa agtacatgtt cccggcgcat ggtaagacgc
    18421 ggcttctgac tccagtaagg ccatgcggtc cactttacct tcggcataaa gctggcgaat
    18481 acgcactttt tctttatttg gcaaaccgct tgccatcggt ccagacggca caaacaccgc
    18541 aggcaaatga ccaaacgaca gggctgccat cgtcagaccc gggacaatct tgtcgcacac
    18601 cccgaggaac agagcaccat caaacatgtt atgggacagc ccgacagccg cagacatcgc
    18661 tatcacttcg cggcttagca gcgacaattc cattccatcc tgcccctggg tgacaccatc
    18721 acacatcgcc ggaacaccgc ccgcaacctg accaaccgca ttcgcttcat gcagggcttt
    18781 acgaatgatt tctggatagt gttcataagg ctggtgagcg gagagcatgt cgttatagga
    18841 ggtgatgatg gcgatattgt tacgcaacat gcttttcaaa gaggctttgt cttctggctg
    18901 gcaggcagcg aaaccgtgtg ccaggttacc gcatgccaac tgcgaacgat gaacggtcga
    18961 cgttttcgct tgttctatcc gggcgagata agcagagcga gtctcgcgcg aacgttcaat
    19021 gattcgattt gttacgcgta acaattgtgg attcataaag gctcctgaaa ttgagttgtc
    19081 agagcaggat gattcacaac gcgtttcatt cagaggattt atgagtgaaa cgcctgtaac
    19141 cggagctcat agggcaaaaa cgtttcagtc agtgtaataa aaaaagcctc gtgggtgaat
    19201 ccgcacgagg cctgaaagtg taaaaattgt tctacaatct gtgcaagatc atgttaccgg
    19261 taaaataacc ataaaggata agcgcagata ttactcaaac tcattccagg aacgaccatc
    19321 acgggtaatc atcgccaccg aggcaacggg tccccaggtt ccggcctgat acggtttcgg
    19381 cgcatcattg tccatcgccc acgcctcagt aatggagtct acccatttcc aggcttcttc
    19441 cacttcatcg cgacgtacaa acagtgcctg aataccacgc atggtttcca gcagcaaacg
    19501 ttcataggca tccgccagat gcgtctgatt aaaggtttct gaatagctca gatccagctt
    19561 ggtgatttgc aggttatgtt tgtggtcaag gccaggaact ttattcagta cctggatatc
    19621 cacgccttca tcaggttgca gacggatagt cagtttattc tgcggcagat cctgccacga
    19681 ctctttaaac agattcagtt caggtgtttt gaaatagacc acgacttcag aacatttggt
    19741 cggcagacgt ttaccagtac gcaggtagaa tggcacaccg gcccagcgcc agttatcaat
    19801 gtcgacgcgg atcgccacga aggtttctgt attgctgctc ttgttcgcgc cctcttcttc
    19861 cagatatccc ggcacttttt tgccctgggc gaagcccgca gtatattgcc cgcgtacggt
    19921 tttttcgcgt acgttggagc ggtcgatgcg gcgcagagac ttcagtactt tcactttttc
    19981 atcgcggatg ctgtctgcgc tcaggtcaga cggcggagac atcgcaatca tgcaaagaat
    20041 ttgcagcagg tggttctgga tcatgtcgcg catctgaccg gctttatcaa aatagcccca
    20101 gcgcccttcg atccccactt cttctgccac ggtaatctca acatgatcaa tggtgcgatt
    20161 gtcccagtta ttcacaaaca gggagttagc aaaacgcagc gccaacaggt tcagcaccgt
    20221 ttctttacca agatagtggt cgatacggta aacctggcac tcctcgaagt attcgccaac
    20281 ctgatcattg atttcctgcg aggtcgccag cgacgtcccc agcggtttct ccatgactac
    20341 gcgtgccggt ttagcattca gttttgcctc gccaagccct ttgcaaattg cgccaaaagt
    20401 gctgggcggc atggcaaagt agttaatggt gatacgattt ttttgatcca gcatcgcgcc
    20461 gagacggctg aatgcagcag tgtcattgac atcgagatta caaaaatcca gacgtgcgct
    20521 cagggtgtcc cataaacctt catcaatggt ttctttcatg aaagtttcga gcgcctcgcg
    20581 gacaactttg gtatatgccg ctttatccca gtcagcacgc cctacgccga taatccgggt
    20641 gtccgggttg agctgaccgg ctttttccag ttgatacagg gaaggcagca atttacgacg
    20701 cgcaaggtcg cctttcgcgc cgaaaatgac caagtcacag acctgggctg tttgcgttac
    20761 cgccatgtca ttctccttaa gttaactaac ccggtactta agccagggta tacttgtaat
    20821 tttcttacgg tgcactgtac tgcttttacg agcttgcgaa aactgtaaac gcttatccac
    20881 ccgtgcgatt acggtaaaag cgcgcaaagt gcggcaaaac tgataaaaaa atcatcgttt
    20941 tccattgggt aaaaatctga cactgatcat gttatgaaaa aaaataacaa cttttttatc
    21001 tgcttttgtc attaacacag cacacaggcg taatatctga caaaactgca tttcgatttc
    21061 tttcagtgcg gaaatcgtca ttacccgtga gtctctttac atcatgaata tgctggaaaa
    21121 aatccagtct cagctggaac atttgagcaa atcagagcgc aaagttgccg aggtcattct
    21181 ggcttcgccc gataacgcga tccattcgag tattgctgct ctggcactgg aagccaatgt
    21241 tagcgaaccg acggtgaatc gtttctgtcg cagcatggac acgcgcggtt ttcctgattt
    21301 taaacttcat ctggcacaga gtctggcgaa tggcactccc tatgttaatc gcaacgtcaa
    21361 tgaagatgac agcgttgaat catacacagg gaaaatattt gagtccgcaa tggcaacgct
    21421 cgatcatgtc cgtcattcac tggataaatc tgccatcaac cgcgccgtcg acttgctcac
    21481 tcaggcaaaa aaaatcgcct ttttcggatt aggctcttca gccgccgttg cccacgatgc
    21541 gatgaataag ttctttcgtt ttaatgttcc ggtggtgtac tccgatgata ttgtgctgca
    21601 acgcatgagt tgtatgaatt gtagcgacgg agacgtggtg gtgctgattt ctcacaccgg
    21661 aagaacaaaa aatctggtcg agctggcgca gctggcacgc gaaaacgacg ctatggtgat
    21721 tgccctcacc tctgcgggta ccccgctcgc ccgggaagca acgctggcaa ttaccctcga
    21781 cgtaccggaa gatactgaca tttatatgcc catggtttct cgacttgcac agctgaccgt
    21841 gatagatgtg ctggcgacag gatttacttt gcgacgcggt gcaaaattca gagataactt
    21901 gaagcgggtc aaggaagcgc tgaaggaatc gcgttttgat aagcagttac ttaatttaag
    21961 tgacgatcgc taaaagcgac tgtcactgtt ctaatcttat acgacatccg aatgagatta
    22021 ttttatcgcc atcgcggcgt tatttcattc ggatttcatg ttcaagcaac acctggttgt
    22081 ttcagtcaac ggagtattac atgtccagaa ggcttcgcag aacaaaaatc gttaccacgt
    22141 taggcccagc aacagatcgc gataataatc ttgaaaaagt tatcgcggcg ggtgccaacg
    22201 ttgtacgtat gaacttttct cacggctcgc ctgaagatca caaaatgcgc gcggataaag
    22261 ttcgtgagat tgccgcaaaa ctggggcgtc atgtggctat tctgggtgac ctccaggggc
    22321 ccaaaatccg tgtatccacc tttaaagaag gcaaagtttt cctcaatatt ggggataaat
    22381 tcctgctcga cgccaacctg ggtaaaggtg aaggcgacaa agaaaaagtc ggtatcaact
    22441 acaaaggcct gcctgctgac gtcgtgcctg gtgacatcct gctgctggac gatggtcgcg
    22501 tccagttaaa agtactggaa gttcagggca tgaaagtgtt caccgaagtc accgtcggtg
    22561 gtcccctctc caacaataaa ggtatcaaca aacttggcgg cggtttgtcg gctgaagcgc
    22621 tgaccgaaaa agacaaagca gacattaaga ctgcggcgtt gattggcgta gattacctgg
    22681 ctgtctcctt cccacgctgt ggcgaagatc tgaactatgc ccgtcgcctg gcacgcgatg
    22741 caggatgtga tgcgaaaatt gttgccaagg ttgaacgtgc ggaagccgtt tgcagccagg
    22801 atgcaatgga tgacatcatc ctcgcctctg acgtggtaat ggttgcacgt ggcgacctcg
    22861 gtgtggaaat tggcgacccg gaactggtcg gcattcagaa agcgttgatc cgtcgtgcgc
    22921 gtcagctaaa ccgagcggta atcacggcga cccagatgat ggagtcaatg attactaacc
    22981 cgatgccgac gcgtgcagaa gtcatggacg tagcaaacgc cgttctggat ggtactgacg
    23041 ctgtgatgct gtctgcagaa actgccgctg ggcagtatcc gtcagaaact gttgcagcca
    23101 tggcgcgcgt ttgcctgggt gcggaaaaaa tcccgagcat caacgtttct aaacaccgtc
    23161 tggacgttca gttcgacaat gtggaagaag ctattgccat gtcagcaatg tacgcagcta
    23221 accacctgaa aggcgttacg gcgatcatta ccatgaccga atcgggtcgt accgcgctga
    23281 tgacctcccg tatcagctct ggtctaccaa ttttcgccat gtcgcgccat gaacgtacgc
    23341 tgaacctgac tgctctctat cgtggcgtta cgccggtgca ctttgatagc gctaatgacg
    23401 gcgtagcagc tgccagcgaa gcggttaatc tgctgcgcga taaaggttac ttgatgtctg
    23461 gtgacctggt gattgtcacc cagggcgacg tgatgagtac cgtgggttct actaatacca
    23521 cgcgtatttt aacggtagag taagtacgtt gccggatgcg gcgaaaacgc cacatccggc
    23581 ctacagttca atgatagttc aacagatttc gaatattctg aagcaaactt gaacttatca
    23641 tcaggcgaag gcctctcctc gcgagaggct tttttatttg atgggataaa gatctttgcg
    23701 cttatacggc tggatttcgc ccggtttgcg agttttcagc aattttagta tccaggtgta
    23761 ttgttctggt cgcggaccaa caaaaatctc gacttcttca ttcatccgcc gcgcaatcgt
    23821 atgatcatcc gcctctaaca gatcatccat cggtgggcgc acctgaatcg tcagacgatg
    23881 cgtcttgcca tcataaatcg gaaacagcgg tacaacgcgc gcacggcaca ctttcatcaa
    23941 acgaccaatc gcgggcaacg tcgctttata ggtggcaaag aaatccacaa attcgctgtg
    24001 ctctgggcca tgatcctgat cgggtaaata atatccccag tacccctgac gtaccgactg
    24061 gatgaatggt ttaataccgt catttctcgc atgcagacga ccgccaaagc gacgacgcac
    24121 cgtgttccag acataatcaa aaaccgggtt gccctgatta tggaacatcg ctgccatttt
    24181 ctgcccttgc gaggccatca gcatggcagg aatatcgacg gcccaaccgt gcggcactag
    24241 aaagataact ttctcgttat tacgccgcat ctcttcgatg atctccagcc cttgccagtc
    24301 aacgcgcggc tgaattttct ccggcccgcg tattgccaac tcagccatca ttgccatcgc
    24361 ttgcggcgcg gtggcaaaca tctcatcaac aatcgcttcg cgttcagctt cactacgttc
    24421 tggaaagcag agcgacagat tgattaacgc acgacggcgt gagctttttc ccagtcgtcc
    24481 ggcaatacgt cccagccgtg ccagaatggg atcacggaac tttggcggcg ttaaagcgat
    24541 acccgccatc gctgctacgc ccagccatgc tccccagtag cgcgggtggc gaaaggattt
    24601 atcaaactca ggaatgtatt cgctattatt ttttttcgtt tccatgcttt tccagtttcg
    24661 gataaggcaa aaatcaatct ggtgatagtg tagcggcgca acttgccccg caccaaataa
    24721 aaaaagccgg tactgactgc gtaccggctg cgaatggatg ttaattaatc aaaccgtagc
    24781 tgcggcacaa tctctttggc ctgcgccagg aattcgcgac gatcggagcc ggtcagccct
    24841 tcggtacgcg gcagttttgc cgtcagcggg tttacggcct gctggtttat ccatacttca
    24901 tagtgcagat gcggcccggt tgaacgtccg gtattaccgg aaagcgcgat acggtcgcca
    24961 cgtttcacct tctgccccgg tttcaccaga atcttgcgca agtgcatata acgcgtggtg
    25021 tagctgcgac catgacgaat agccacataa taacctgctg cgccactgcg tttggcaacc
    25081 accacttcac cgtcacccac tgaaagcact ggcgtaccct gcggcatggc gaaatcaaca
    25141 cctctgtgtg gtgcaacgcg accggtcacc ggattagtac gacgcgggtt aaagttagac
    25201 gagatacgga actgtttcgc cgtcgggaat cgcaagaatc ctttcgccag accagtaccg
    25261 ttacgatcgt agaatttgcc atcttcagcg cggatagcgt aataatcttt accttctgaa
    25321 cgcaaacgta cgcccagcag ctggctttgc tcacgtttac catcaagcat ttctcgagac
    25381 attaataccg caaattcatc gccttttttc agtttgcgga aatccatttg ccactgcatg
    25441 gctttaatca ctgcgctcac ttcggcgctg gtcaaaccgg cgtttctggc gctggcaaca
    25501 aagctccccc cgacggtacc tttcagcaga ttgttaaccc actctccttg ctgcatttcg
    25561 ctggtcattt taaaaccgtt agcggcagta cggtcatagg ttcgggtttc acgacgagac
    25621 acttcccagg tgaggcgctg cagttcgccg tccgcggtta atgtccagga gagttgttga
    25681 ccgattttca ggttacgcaa ttctttgtcg gccgcagcca gttgggtgat atcacccata
    25741 tcaataccat actgattgag aatgctgctt agcgtatcgc cagtggaaac aacatattca
    25801 tgcacgcccg cttcaccggc gattttgtca tccagttcgt cctggggaat ggcttcatct
    25861 tcttgtgcag cttgatcaat cggctcactg gcttcaggta agagcgaacg aatttcgttc
    25921 tgttccagct caatggtttt gacaattggc gtggcatcgc ggtgataaac atagggccgc
    25981 cagacagcga cggccagagt aagaacggtg agcgacccca acataacgcg gtgtggtcgc
    26041 ggtaaattat taaacgccag ggcgacagag cgggctatct gttgcacgta atcacttcct
    26101 cattaatctc ctttcaggca gctcgcatac tggttggcta attgactcag gaattctgaa
    26161 tagcttgttt tacccagttt gatattcgtc cccaagggat ccaacgttcc catacgaacg
    26221 gatgtccctc gtgcgacgct ctcaacgacc gctggcctga actgtggctc agcaaaaacg
    26281 caggttgctt tttgctcaac caactgtgtt cttatttcat gtaaacgctg cgcgccaggt
    26341 tgaatctcag ggttaacggt aaaatgacca agcggtgtca gtccgaactg tttttcgaaa
    26401 tagccgtaag catcgtgaaa aacgaaataa cctttcccct tgagcggcgc gagctcgtta
    26461 ccaacctgcg tttcggttga ggctaattgt gcctcaaaat ccttcaggtt ggcgtcaagt
    26521 ttggctcgac tttgcggcat aagttccact aattttccat ggattgcaac cgctgtagcc
    26581 cgcgctatct ctggggaaag ccaaagatgc atgttgaaat cgccgtgatg gtgatcttcg
    26641 tcactttttt ccgcgtggtc gtgatcatca tcatcgccgt taatactttt catcagtagc
    26701 ggtttcacat cttcaagctg cgcaatcgtt acctgcttcg cttctggtaa tttgcttacc
    26761 ggtttttgca taaacgcttc catctccggg ccaacccaaa cgactaagtc cgcgttctgt
    26821 aagcgtttta catccgatgg acgcagtgaa taatcatgtt ctgaagcgcc gtcaggaagt
    26881 aaaacctccg tttctgttac cccatcagca atggcagaag cgatgaaccc aacgggttta
    26941 agcgaagcga caacggcggc atctgcggcc tgtgttgcac cgccccagag agcggcgaat
    27001 aatgctgcga aaagaagcgt ttttttatgt aacataatgc gaccaatcat cgtaatgaat
    27061 atgagaagtg tgatattata acatttcatg actactgcaa gactaaaatt aacatgacaa
    27121 gtctggtttc cctggaaaat gtctcggttt cttttggcca acgccgcgtc ctctctgatg
    27181 tgtcgctgga acttaaacct ggaaaaattt tgactttact tgggccaaat ggcgcaggta
    27241 agtcgacact ggtacgggta gtgctcgggc tggtaacacc cgatgaaggg gttatcaagc
    27301 gcaacggaaa actgcgcatc ggctatgtac cgcagaagct gtatctcgac accacgttgc
    27361 cactgaccgt aaaccgtttt ttacgcttac gccctggtac acataaagaa gatattttgc
    27421 ctgcactgaa acgtgtccag gccgggcacc tgattaacgc accgatgcaa aagctctctg
    27481 gtggcgaaac gcagcgtgta ctgttagcgc gagcattgtt aaatcgcccg caattattag
    27541 tgctggatga acccactcaa ggcgtggatg taaatggcca ggtggcgtta tatgacctta
    27601 ttgaccaact gcgtcgtgaa ctggattgtg gcgttttaat ggtttctcac gatcttcatc
    27661 tggtgatggc aaaaaccgat gaagtgctgt gcctgaatca tcacatttgt tgttccggca
    27721 caccggaagt tgtttccctg catccggagt ttatttcaat gtttggtcct cgtggtgctg
    27781 aacaactggg tatctatcgc catcatcata atcatcgtca cgatttacag ggacgaattg
    27841 ttttgcgtcg gggaaatgat cgctcatgat tgaattatta tttcccggtt ggttagccgg
    27901 gatcatgctc gcctgtgccg cgggtccgct gggttcgttt gtagtctggc gtcgtatgtc
    27961 ttatttcggt gatacgctgg ctcatgcctc attacttggt gtcgcgtttg gtttgttgct
    28021 ggacgtgaat ccattctatg cggtgattgc cgttacgctg ctgctggcgg gcggtctggt
    28081 atggctggag aagcgtccac agctggcgat cgacacgtta ttagggatta tggcgcacag
    28141 tgccctgtcg ctgggcctgg tggtcgttag tctgatgtct aacattcgtg ttgatttgat
    28201 ggcttacctg ttcggtgatt tactggcagt gacgccagaa gatctcatct ctattgcaat
    28261 tggcgtggtc atcgtggtgg ctattttgtt ctggcaatgg cgcaatttgc tgtcgatgac
    28321 gattagcccg gatctggcgt ttgttgatgg tgtgaaatta cagcgagtga aattgttgtt
    28381 gatgctggtg acggcattga cgattggtgt agcgatgaaa tttgtcggtg cgttgattat
    28441 tacttcgttg ctgattattc ctgcagctac tgcgcgtcgc tttgcccgta cgccggaaca
    28501 gatggctggt gtcgctgttt tggtggggat ggtggcagta actgacggtt taaccttttc
    28561 cgcgttttac gatacgccgg cgggtccgtc ggtggtgcta tgtgcggcac tgttatttat
    28621 tatcagtatg atgaaaaagc aggccagcta atctatcgct gaacacattt gtcggatgcg
    28681 gcgcgagcgc cttatccgac ctacggttcg ctatctctgg taggcctggt aagacgcgaa
    28741 cagcgtcgca tcaggcacac tgccaatgtc ggatgcggcg cgagcgcctt ttccgaccta
    28801 cggttcgcta tctctggtat atgcctgata agacgcgaat agcgtcgcat caggcacact
    28861 gccaatgtcg gatgcggtgc gagcgaccaa tccgacttac ggcatttctg gcggcgttat
    28921 gccaaagtga ttccacgccc gcgtcgtcgc catacgccca cgcggtgtac gctgcaaaaa
    28981 gccttgctga atcaaataag gttccagcac atcctcaatg gtttcacgtt cttcgccaat
    29041 ggctgccgca aggttatcca gacctaccgg cccaccaaag aacttatcga ttaccgccag
    29101 caacaatttg cggtccatat aatcgaaacc ttcagcatcg acattcaaca tatccagcgc
    29161 ctgagcagcg atatctgccg agatggtgcc atcgtgcttc acttcggcga aatcacgcac
    29221 tcgacgcagc agacggttgg caatgcgcgg cgtaccgcga gcgcgacgag caacttccag
    29281 cgcgccgtca tcactcatct caagccccat aaagcgtgcg ctgcgactga cgatatattg
    29341 cagatccggc acctgataaa actccagacg ttgcacaata ccaaaacggt cgcgcaacgg
    29401 tgatgtcagc gaacctgcgc gcgtggttgc accaatcagg gtaaacggcg gcaaatcaat
    29461 tttaatggag cgtgccgccg gaccttcacc aatcatgata tccagttggt agtcttccat
    29521 tgccgggtac agcacttctt caacaactgg cgataaacgg tggatctcat caataaacag
    29581 cacgtcatgc ggttcaaggt tagtgagcat cgcagccaaa tcgcccgcct tttccagcac
    29641 cggaccagaa gtcgtgcgta aattaacgcc catttcattg gcgacaatgt tggcaagcgt
    29701 agttttaccc aaccccggag gaccaaaaat caacaaatga tcgagggcat cgccgcgcag
    29761 tttcgctgct ttgatgaaaa tctccatctg tgaacgaacc tgcggctgac caacatactc
    29821 ttccagtaat ttagggcgaa tggcgcgatc tgctacatct tccggcaaag tggtaccggc
    29881 agaaatcaga cggtctgctt caatcatcct ttacctcata acgcggcgcg tagggcttcg
    29941 cgaattaatg tttcactgct ggcgtcaggg cgagcgattt tgctcaccat gcggcttgct
    30001 tcttgtggtt tatagcccag cgccaccagc gcggcaaccg cttcttgttc agcatcgtcg
    30061 gtcgccgggc tggcaggaga cgtgagtacc aggtcggcgg ctggcgtaaa gagatcgcca
    30121 tgcaaacctt taaatcggtc tttcatttca acaatcaagc gttcggcggt ttttttgcca
    30181 atacccggca gtttcaccag tgcccccact tcttcacgct caacggcatt aacgaactgc
    30241 tgcgctgaca ttccggagag gatcgccagc gccaacttcg ggccgacgcc gttggttttg
    30301 atcaactctt tgaacaacgt gcgctcttgt ttattgttaa aaccgtacag cagttgcgcg
    30361 tcttcacgca ccacaaagtg ggtgaaaacg atcgcttcct gacccgcttc agggagttca
    30421 taaaaacagg tcatcggcat atgcacttca tagcctacgc cgcccacttc aattaacacc
    30481 agcgggggtt gtttttcaat gatgatgcct ctgagtctgc ctatcacatg acgctcctgc
    30541 gtaatgaatc aaagataatg ctgtatgata aaaaaatgct ggatagatat ccagcgaagg
    30601 atgaaaaaaa cttgcgaggt gtctcgatga actgaaaaat ggca
//
LOCUS       DAZFDC010000049        30242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFDC010000049 DAZFDC010000000
VERSION     DAZFDC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.049"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(75..794)
                     /gene="deoD"
                     /locus_tag="ACHJ48_002294"
     CDS             complement(75..794)
                     /gene="deoD"
                     /locus_tag="ACHJ48_002294"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO4707850.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(851..2074)
                     /gene="deoB"
                     /locus_tag="ACHJ48_002295"
     CDS             complement(851..2074)
                     /gene="deoB"
                     /locus_tag="ACHJ48_002295"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO4707851.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(2126..3448)
                     /gene="deoA"
                     /locus_tag="ACHJ48_002296"
     CDS             complement(2126..3448)
                     /gene="deoA"
                     /locus_tag="ACHJ48_002296"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO4707852.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(3575..4354)
                     /gene="deoC"
                     /locus_tag="ACHJ48_002297"
     CDS             complement(3575..4354)
                     /gene="deoC"
                     /locus_tag="ACHJ48_002297"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO4707853.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            4612..6161
                     /gene="yjjI"
                     /locus_tag="ACHJ48_002298"
                     /pseudo
     CDS             4612..6161
                     /gene="yjjI"
                     /locus_tag="ACHJ48_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
     gene            6133..6996
                     /gene="yjjW"
                     /locus_tag="ACHJ48_002299"
     CDS             6133..6996
                     /gene="yjjW"
                     /locus_tag="ACHJ48_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO4707854.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(7210..7992)
                     /gene="yjjV"
                     /locus_tag="ACHJ48_002300"
     CDS             complement(7210..7992)
                     /gene="yjjV"
                     /locus_tag="ACHJ48_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4707855.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGESGLDL
                     FGDDPQFERQQWFLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRVVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFNVRG"
     gene            complement(7989..9062)
                     /gene="yjjU"
                     /locus_tag="ACHJ48_002301"
     CDS             complement(7989..9062)
                     /gene="yjjU"
                     /locus_tag="ACHJ48_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO4707856.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(9184..9345)
                     /gene="ytjA"
                     /locus_tag="ACHJ48_002302"
     CDS             complement(9184..9345)
                     /gene="ytjA"
                     /locus_tag="ACHJ48_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO4707857.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(9472..10077)
                     /gene="osmY"
                     /locus_tag="ACHJ48_002303"
     CDS             complement(9472..10077)
                     /gene="osmY"
                     /locus_tag="ACHJ48_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO4707858.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGIVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(10470..12059)
                     /gene="prfC"
                     /locus_tag="ACHJ48_002304"
     CDS             complement(10470..12059)
                     /gene="prfC"
                     /locus_tag="ACHJ48_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO4707859.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(12150..12827)
                     /gene="yjjG"
                     /locus_tag="ACHJ48_002305"
     CDS             complement(12150..12827)
                     /gene="yjjG"
                     /locus_tag="ACHJ48_002305"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO4707860.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(12842..13288)
                     /gene="rimI"
                     /locus_tag="ACHJ48_002306"
     CDS             complement(12842..13288)
                     /gene="rimI"
                     /locus_tag="ACHJ48_002306"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO4707861.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(13257..13670)
                     /gene="holD"
                     /locus_tag="ACHJ48_002307"
     CDS             complement(13257..13670)
                     /gene="holD"
                     /locus_tag="ACHJ48_002307"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO4707862.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            13773..14804
                     /gene="rsmC"
                     /locus_tag="ACHJ48_002308"
     CDS             13773..14804
                     /gene="rsmC"
                     /locus_tag="ACHJ48_002308"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO4707863.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFQHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            14994..15080
                     /locus_tag="ACHJ48_002309"
     tRNA            14994..15080
                     /locus_tag="ACHJ48_002309"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15028..15030,aa:Leu,seq:cag)
     gene            complement(15119..15355)
                     /gene="yjjZ"
                     /locus_tag="ACHJ48_002310"
     CDS             complement(15119..15355)
                     /gene="yjjZ"
                     /locus_tag="ACHJ48_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO4707864.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            15496..16284
                     /gene="fhuF"
                     /locus_tag="ACHJ48_002311"
     CDS             15496..16284
                     /gene="fhuF"
                     /locus_tag="ACHJ48_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO4707865.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(16322..16999)
                     /gene="bglJ"
                     /locus_tag="ACHJ48_002312"
     CDS             complement(16322..16999)
                     /gene="bglJ"
                     /locus_tag="ACHJ48_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO4707866.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(16957..17682)
                     /gene="yjjQ"
                     /locus_tag="ACHJ48_002313"
     CDS             complement(16957..17682)
                     /gene="yjjQ"
                     /locus_tag="ACHJ48_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4707867.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            18303..19073
                     /gene="yjjP"
                     /locus_tag="ACHJ48_002314"
     CDS             18303..19073
                     /gene="yjjP"
                     /locus_tag="ACHJ48_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO4707868.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQHPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            19064..19537
                     /gene="yjjB"
                     /locus_tag="ACHJ48_002315"
     CDS             19064..19537
                     /gene="yjjB"
                     /locus_tag="ACHJ48_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO4707869.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            19644..20183
                     /gene="dnaT"
                     /locus_tag="ACHJ48_002316"
     CDS             19644..20183
                     /gene="dnaT"
                     /locus_tag="ACHJ48_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO4707870.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVDLDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            20186..20923
                     /gene="dnaC"
                     /locus_tag="ACHJ48_002317"
     CDS             20186..20923
                     /gene="dnaC"
                     /locus_tag="ACHJ48_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO4707871.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            20972..21466
                     /gene="yjjA"
                     /locus_tag="ACHJ48_002318"
     CDS             20972..21466
                     /gene="yjjA"
                     /locus_tag="ACHJ48_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO4707872.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDVENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            21720..24011
                     /gene="opgB"
                     /locus_tag="ACHJ48_002319"
     CDS             21720..24011
                     /gene="opgB"
                     /locus_tag="ACHJ48_002319"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO4707873.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(24148..25170)
                     /gene="lgoD"
                     /locus_tag="ACHJ48_002320"
     CDS             complement(24148..25170)
                     /gene="lgoD"
                     /locus_tag="ACHJ48_002320"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO4707874.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITAGMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            25309..26223
                     /gene="lgoR"
                     /locus_tag="ACHJ48_002321"
     CDS             25309..26223
                     /gene="lgoR"
                     /locus_tag="ACHJ48_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO4707875.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            26438..27799
                     /gene="lgoT"
                     /locus_tag="ACHJ48_002322"
     CDS             26438..27799
                     /gene="lgoT"
                     /locus_tag="ACHJ48_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4707876.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(27848..29512)
                     /gene="tsr"
                     /locus_tag="ACHJ48_002323"
     CDS             complement(27848..29512)
                     /gene="tsr"
                     /locus_tag="ACHJ48_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO4707877.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            29741..>30242
                     /locus_tag="ACHJ48_002324"
     CDS             29741..>30242
                     /locus_tag="ACHJ48_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707878.1"
                     /translation="MLNRDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVE
                     FANMLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDA
                     TSCVKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKS
                     RKKHVLL"
BASE COUNT         7314 a         7993 c         7485 g         7450 t
ORIGIN      
        1 tgaatagata ctaaaaagcc ggagcagtcc cccactccgg cttaacacga caatcgcctt
       61 tcagcgaaac acaattactc tttatcgccc agcagaacgg attccagtgc gattttgatc
      121 atgtcgttga aggtggtctg acgctcagcg gcagtggttt gctcgtgagt gcggatgtgg
      181 tcagacacgg tgcagatggt cagggctttc gcgccaaact ctgcagccac gccgtagata
      241 ccagccgctt ccatttccac gccgagaatg ccgtattttt ccatcacgtc gaacatttcg
      301 ccgtccggag agtagaacag gtcagcggag aacaggttac caacgcgagc atcaatgccc
      361 agtgctttag ctgcatctac tgcgttacgc accatgtcga agtcagcgat agcggcaaag
      421 tcatggtctt taaaacggat gcggttaact ttggaatcgg tgcaggcacc cataccgata
      481 acgacgtcgc gcagttttac gtgcggcaga actgcgccac aggaacccac gcggataatt
      541 ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga tggagcagga cgggataccc
      601 ataccgtgac ccattacgga aattttgcgg cctttgtaag taccggtgaa gcccagcata
      661 ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag tttcagcaat atacttcgca
      721 cgcagcgggt cgcctggcat caaaactacg tcagcgaaat cgcccatttc tgcattaatg
      781 tgtggggtag ccattgtttt atcctttttg tgacataaca aaggcggagt caacgctccg
      841 cccaaatcca tcagaacatg gctttgccat attccatatc agaagtacca aaatattttg
      901 ccagagtctg gccgatatcc gcgaaggttt cacggtgacc cagtgagccc ggttttactt
      961 tcgggccgta aaccagtacc ggaatgtgtt cacgcgtgtg gtcagtaccg gtccaggtcg
     1021 gatcacagcc gtggtcagcg gtgaggatca ggatgtcgtc atcgcgcagc agagacatca
     1081 gctccggcag acggcggtcg aacagttcca gacccgcagc ataaccggcg acgtcgcgac
     1141 ggtggcccca ggaagagtcg aagtcaacga agttggtgaa gacgatggtg ttatcacccg
     1201 cttctttcat ctctttgatg gtggcgtcaa acagcgcgtc caggccagtc gctttcactt
     1261 ttttggtgat accgcagttg gcgtagatgt ctgcaatttt accgaccgaa accacctggc
     1321 cgtgtttttc atcaaccagt ttctgcagca cggtcggtgc tggcggctca acagccaggt
     1381 cgtgacggtt accggtacgc tggaagttac cggctttgtc gccgataaac ggacgagcga
     1441 taacacgacc gatattgtag ccgccgttgg tcagctcttc acgggcgatt tcgcacagtt
     1501 cgtagagttt atccagaccg aaagtttctt catggcaggc aatctggaac acggagtcag
     1561 cggaggtata gaaaatcggc ttgccggttt tcatgtgctc ttcgcccagt tgatccagaa
     1621 tgaccgtacc ggaagagtgg cagttaccga ggtaacccgg cagattagcg cgttcgacca
     1681 gtttatccag cagctcttgc gggaagctgt tttcgtgatc ggagaaatat ccccactcaa
     1741 acagaaccgg gacaccggca atttcccagt gaccagacgg ggtatcttta ccggatgaca
     1801 tttcgtgcgc ccatgcgtac gcgccgataa cttcagcgtt gccgtccatt cccgccggaa
     1861 tgaaaccggt agaaccttcg tgtgctttcg ccagccccag acgggtcaga tttggcagat
     1921 tgagcgggcc tttacgaccg ttatcagctt cgcctttggc acaagcttct gcgatatgac
     1981 ccagggtgtc agccccgacg tcaccaaagc gttctgcatc ttctgtagcg ccgatgccga
     2041 atgagtccag caccataata aatgcacgtt tcatatgttc tccgtactca gtgcttcaaa
     2101 tttaaatgat cagatcagta taccgttatt cgctgatacg gcgatagaca gttggtgtgc
     2161 tttccggtgc tttatcggca agtttaattg ccgctttcac cgctttcgcc gcttcctgcc
     2221 agctgttttc gtctttcgcg tggataaccg ccagcggacg ctggccgtct acctggtcgc
     2281 ccagacgtgc catatcagta aagccgacgc tgtaatcgat ggtgtcagat gcctgacggc
     2341 gtccgccgcc cattgcaacc actgccatcc ccagcgcgcg ggtatccatt tcactgacaa
     2401 aaccttcggt atcagcatag actgctttcg tcagcatcgc tgtcggcaga tacttcgcgt
     2461 agttctcaac gaagtcggtc gggccttttt gtgccgctac catacgacca aagacttctg
     2521 ccgctttacc gttgtccagc accgcctgca atttcgcgcg cgcttcggcg tcatctttcg
     2581 ccagtttgcc ggagataagc atctccacac acagcgccat cgtgacatca aacagacgcg
     2641 ggttacgata ttcacccgtc aggaactgca ccgcttcacg tacttcaacc gcgttaccgg
     2701 cactggaggc cagcacctga ttcatgtcgg tgagcagtgc ggtggtgcgc acgcctgcgc
     2761 cgttagccac gccaacaatc gcttcggcaa gggcttcaga gagttcgtag gtcggcataa
     2821 acgcgccgct acccactttc acgtccatca ccagcgcgtc cagaccttcc gccagtttct
     2881 tcgccaggat cgaggcagtg atcagcggga tggagtccac ggttgcggta atatcacggg
     2941 tcgcgtagaa acgtttatcc gccggagcca gtgagctggt ctgaccgata atcgccacgc
     3001 cgacgtcttt aataatttcg cggaaacggt tgtcatccgg gaaaatgtcg aagccaggga
     3061 tggattccag tttgtcgagc gtaccgccag tatgaccgag gccgcgacca gagatcatcg
     3121 gaatatagcc gccgcaggct gcgaccatcg gccccaacat cagcgaagtc acatcgccga
     3181 cgccaccggt ggagtgttta tcaacaatcg ggccattcag atgcaggctt ttccagtcga
     3241 gaacggttcc tgaatctcgc atcgccatgg tcagcgagac acgctcaggc attgtcatat
     3301 cgtggaagaa aatggtcatc gcgagggcgg caatctgccc ttcggagata gtgttgtcgc
     3361 gaataccgtt gataaagaaa cgaatttctt catcgctcag cgcatgacca tcacgttttt
     3421 tacgaataat ttcttgtgcg agaaacaagg taacctcccg aggaaaagag tatgaggcgg
     3481 cttttgccgg atgcgtctac gccttatccg gcctacgggt agatacgtct cgtaggcctg
     3541 ataagcgcag cgcatcaggc gtaaagcatc ttacttagta gctgctggcg ctcttaccgt
     3601 cgccgtgacc cagcgctttc agcaggcttg ccagcaggct ggaagcacca aagcggtagt
     3661 gacgcgcatc agcccagtca gcaccgaaca gttcatctgc gatggcgaga tatttctgcg
     3721 catcttccgc agtacgcacg ccgcccgccg gtttgaaacc aacggttttt tctacgccca
     3781 tatcacggat cacttccatc atgatgcgcg cgctttccgg cgtcgcgttc acagccactt
     3841 taccggtaga ggttttgatg aagtccgcac ccgctttgat ggagatttca gacgctttac
     3901 ggatcagcgc ttcgtctttc agttcgccgg tttcgatgat cactttcagc agtacattcg
     3961 ctgccgcgca agcctcttta caggctttca ccaggtcaaa accaacctgc tcgttacccg
     4021 ccatcagcgc gcggtacggg aacacaacgt caacttcatc agcaccgtag gcgattgccg
     4081 cacgggtttc tgccagcgcg atttcgatgt cgtcgttacc gtgtgggaag ttggttaccg
     4141 tagcaatacg gatttccggg gtgccctgct ctttcagtgt tttgcgagca atcgggataa
     4201 agcgaggata gatacagata gcggcggtat tgccgaccgg agttttggcc tgatgacaca
     4261 gagcaattac tttctcgtcg gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca
     4321 gtgcacgcag gctgcttgct ttcagatcag tcatttcatt ctcctggctt gtcgccaata
     4381 aaattcacct tgcgagtttg ttagtattct aacatctact ccgcaacaca cttcgatata
     4441 catcacaatt aaggaaatct acttacaagt ttgcatcact ataatgcgat ctggttcaaa
     4501 taattcactt tcaaatgaat gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat
     4561 cacgaaactt cttacaatgg cgcatcacct tcggcataaa aggaaaagaa gatgcccact
     4621 tctcatgaaa atgcactgca acaacgttgc cagcaaattg tcaccagccc agtgcttagc
     4681 ccggagcaga agcgccattt tctggcactg gaagcagaaa acaatctgcc ttacccacag
     4741 cttcctgccg aagcccgccg cgcgctggat gaaggtgtaa tctgcgatat gtttgaaggt
     4801 catgcgccgt acaaaccgcg ctatgtctta cccgattacg cccgttttct ggcgaacggt
     4861 tccgaatggc tggagctgga aggcgcgaaa gatcttgatg acgcactctc tctgctgacc
     4921 attctttacc accacgtacc gtcggtcaca tcgatgccgg tctacctggg gcaactggat
     4981 gcgttgttgc aaccgtatgt tataattcta acacaagacg agatcgatgt tcgaataaaa
     5041 cgtttctggc gttacctcga cagaaccctg ccagacgcct ttatgcacgc caatatcggc
     5101 ccgtctgatt cgcccattac ccgcgcgatc ttacgtgcag acgcagattt gaagcaggtt
     5161 tcaccgaacc tgacctttat ctacgatcct gaaatcaccc ctgatgacct gctgctggaa
     5221 gtggcgaaga acatctgtga atgtagcaaa ccgcacatcg ccaacggtcc ggtgcatgat
     5281 aaaattttca caaaaggggg ctacgggatt gtgagctgtt acaactcact gccgctggcg
     5341 ggtggtggca gcacgctggt acgccttaac ctgaaagcca ttgccgagcg cagcgaatcg
     5401 ctggatgact tctttacgcg cactctaccg cactactgcc agcagcagat cgccatcatc
     5461 gatgcgcggt gtgaattcct ctatcaacaa tcacacttct ttgagaatag cttcctggtg
     5521 aaagaagggc tgattaaccc tgaacgtttt gtgccaatgt ttggcatgta tgggctggcg
     5581 gaagcggtta acttgctgtg tgaaaaagaa gggattgccg cgcgctacgg taaagaagcc
     5641 gccgcaaatg aagtaggtta tcgcatcagt gcacagctgg cggagtttgt cgccaatacc
     5701 cccgtgaaat atggctggca aaaacgcgcc atgttacacg cacagtcggg gatcagttcc
     5761 gatatcggca ccacgccggg cgcgcgtttg ccgtatggcg atgagccaga tccgatcacc
     5821 catctgcaaa ctgtcgcccg catcatgctt attattattc tggcatcagc gacattctga
     5881 cgctcgacga aaccatcaaa cgtaacccgc aggcgttggt acagctttgc ctcggtgcct
     5941 ttaaagccgg aatgcgtgaa tttaccgcca atgtcagcgg taacgatctg gttcgcgtta
     6001 ccggttatat ggtgcgtttg tcggatttag aaaaatatcg cgccgaaggt tcacgcacca
     6061 acaccacctg gctgggcgaa gaagccgcac gcaacactcg tattctggaa cgccagccgc
     6121 gcgtgataag ccatgaacag cagatgcgct ttagtcagta agattatccc cttctcctgc
     6181 gttgacgggc caggcagtcg tctggctctg tttttgcagg gctgcaatct gcgctgcaaa
     6241 aactgtcaca atccgtggac gatgggacgt tgcaatgact gtggggagtg cgtgccacag
     6301 tgtccgcatc aggcgttgca gattgttgac ggcaaagtgg tgtggaacgc tgtggtctgc
     6361 gagcaatgtg atacctgcct gaagaggtgt ccgcaacatg ccacgccaat ggcgcaatcc
     6421 atgagcgtgg atgaggtgct tagccatgtc cgcaaagcag tgctgtttat cgaagggata
     6481 acggtgagtg gcggcgaagc cacgacccaa ctgccgtttg tagtggcgct gtttactgct
     6541 atcaaaaacg atccgcaact gcgccacctc acctgcctgg tggacagtaa cggtatgttg
     6601 agcgaaaccg gctgggaaaa attgctcccg gtgtgcgacg gcgcaatgct cgatctcaaa
     6661 gcgtggggga gcgaatgtca tcagcaatta accggacgcg ataatcagca gattaagcgc
     6721 agcatctgtt tgctggcaga gcgcggtaag ctggcggaac tgcgtttgct ggtaatccct
     6781 ggccaagtgg attatttgca acacatcgaa gaactggcgg cgtttatcaa gggagtcggc
     6841 gatgttccgg tacgcctgaa cgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc
     6901 tgggcgagcg ccacgccgga agacgttgag ccgttggctg atgcgttacg ggagcgcggg
     6961 gtgagccggt taatatttcc ggcgctgtat ttgtgacatg ccggatacga cctacggttc
     7021 ggcacagact tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat tgtgcgccaa
     7081 ctgccggatg cggcgtgaac gccttatccg gcctacggtt cggcacaaac ctgtaggcat
     7141 gataagacgc gttaagcgtc gcatcaggca ttatgcgccg actgccggat gctgcctacg
     7201 aagggcaaac tacccacgaa cgttaaacac cgcatacgtg ttattaagca acacttcggc
     7261 aatctcatct tctggttccg ggcgcaactc gcaaagcacg tcaaacaccc gcgcagcctg
     7321 ttccgggcgg ttaggctgcc cctgaaaacc gttgagcggc atatccggcg cgtcggtttc
     7381 cagcaacaac gatgccagcg gtaattttgc gatgacaacg cgggttttac tggcgcgtgg
     7441 ataggtgata gtaccgccta cgccgatttt gtagcccagc tgtacaaacc gctcggcctg
     7501 ttgcaggctg ccggaaaaac cgtgaaccac gccagtgcgc gataaatcgt ggcgtttaag
     7561 atgcatcgcc agtttgtcgt gtgtgcgccg tgaatgcagg atcaccggca gatcgtagcg
     7621 tttcgccagt ttcagttgtt cgtcgagaaa ccactgctgc ctctcaaatt gcggatcgtc
     7681 gccgaagaga tccagaccgc tctcccccac cgccaccacc ttcgccggac gcctttccag
     7741 cgcctgctgt agctgatcaa gagacacatc gctatgtttt tccaacatac caggatgcaa
     7801 gcccaatgcg gcatacagcg gttgataatt ttccgctaac gcctgcacgc gggcaaaatt
     7861 ttccgcctcg gttgccggaa caatgatctt gcctacgccc gcttgtgccg cgcgttgcag
     7921 gctggcctct tcatcgccaa taaaaggcgg gaaatcaaaa tggcagtggg tgtcgataaa
     7981 acggcaaatc aagccagatc ctcattgtta aatgtcgggt cgttcgcctg cggagcgtcg
     8041 ctcacttcgg caaccagcgt atcgttggcg actggcgcag gcggaatgac aatcgattcc
     8101 ggcgtcacca ctggcaccag atggcgggta agcggcgctt tttcagttaa tagcttgcca
     8161 accgtggcga ggaaataacg accgcataaa cgcccaagtt tatagtcttc acgcagcgcc
     8221 ggaatccgac tgccaagcgc gatactatgt aatggcttcg gcggataaat ttcgaatatc
     8281 cgcagcttgc ccggtggttt ctcaataaat tgctgaatgt cacgatagct ggtttcatga
     8341 tgctgcacca gattgaccag cggctgcagg ctactgtcac ccagccagcg ttccatgcgt
     8401 ttgaaccact gcggcgtgta gtacatttgt gacggcacag tgcggatgac gaccaacgtt
     8461 ttagcgccct gccttgccgc ctctttaacc ggaatcgcat cactgatccc gccatccagg
     8521 tagttaatgc cttccagcga cactccgcta cgataaaagc caggtatcgc actggaggcg
     8581 cgaatcacat ccagccagtt ttgtttggtt ggtaaaaagt aattcggcgc gtagtcatcc
     8641 tgacgacagg cgcacatata aaacgatttg ccgctgtcaa acaaccgcgc ggcggtatcc
     8701 atttgcaacg gcatctggct tgcggtggcc tccaccagcc agtcgagatc gataagattt
     8761 cctccacgga caaagcgcaa tggatcgaaa aattcgcgtt ttgtggtata gcgcatgatg
     8821 actttgcgcg cgtaaccggg ctgattgcag atatacgccg agaggttctg cgccccggca
     8881 gatgtgccga gataaagatc gaaaggatta aactgcgcgc gcataaactc atccagcacg
     8941 ccagccgtga aaattccacg ctgtccgccg ccttcgcaca ccagagctat tcgtccaggc
     9001 tggaacggcc ttagcgacaa cggcgcaata ttaccaagcg ttacaggtat tcgctgcccc
     9061 acctctgcct tcctgttttt gttgtgaaag aatgacaaag taacgcaatt tattattcgc
     9121 taaaaccgca aaagccagtc cgctggactg gctttggata aaattttctt aatggtctga
     9181 aatctagggt cgttttcggc ccatgaacaa actcaccagg aacagaataa tcccgacgac
     9241 aaagacaatt ttagctgcgc ctgcagcggt accggccaga ccaccaaacc caagtgcggc
     9301 ggcgattaac gcgataacca gaaatatgat gccccaacga aacataagcg tctcctttac
     9361 catagttaat gtcaccgctt gatatgagcg tgaaaatcac tcaatgcgat atttttagtg
     9421 tggtgcacat cgcgcctccc gacaaagttc gggaggacga attacgacga attacttagt
     9481 tttcagatca tttttaacgc ttttcacacc atctaccgct ttggcgatac tttcagcacg
     9541 gtcactttgt gcctgagaat ctacgatacc agagagctga accacgccat cggtggtttc
     9601 aactttcaca tgacgggaag ggacgatatc gtccgccagc aatttggctt tgatttcact
     9661 ggtggtggcg gtgtcacccg cgtagccctt cacagagcct tctttagcgt cgcgaacgtg
     9721 cagtttgtcg ctgacagagg tcaccccttc aacacctttc gccactttca ctgcctcttc
     9781 ggcctgggcc tggctttcaa cgaaaccgct cagggtcacg actttttgat cggttttgac
     9841 agagatatcg gtgctcttga tgttgtcatg atccaccagc gccgccttca ctttcgcggt
     9901 gatggcgctg tcatccatga aattaccgac tttattcata gagctatcga ctttttgccc
     9961 tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc
    10021 agaggtcaac attacagcca gcagagtttt cgaaatcttc agtcttgtca tagtcatcga
    10081 tttattcctg tatgtttgct cgtaatttga gcctggcaac acgaggttgc attgctgaat
    10141 agggagagac ttcaccctct acagaagtca atggtcgcca tcacaaaagc gatgagtgat
    10201 gaataacgac cattacagcc tctgaatcag ttattaatat cggtagaatg acaatcgacg
    10261 gctttagata ctgatatcta cgcattgaac ggtatttaac gccgtcagaa atgtcatcac
    10321 tttgttaaat atagatcaca attttgaaac cgctcgggat atcagcgaaa acataagcaa
    10381 aagtgaatgt tttaagaaca ttccgtaagc ggctaataag gaagggaaat tgacaggacg
    10441 cagcggctac cgcgccctgg aggcaagaat taatgctcgc gggtctggtg gaactgaacg
    10501 tccggataac gttcctgtgc caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg
    10561 ttatcgccgc catcaagcgc cagttggctt tcgttcttac gcttgaactc ttcgaatttc
    10621 ttcgcgtctg cacattctac ccagcgggca gtggcaacgt tgactgactc atacactgct
    10681 tcaacgttgt attcgctctt caggcgcgct accaccacat caaactgcag cacaccaacc
    10741 gcaccaacga tcagatcgtt gttggagatt ggacggaaca cctgcaccgc gccctcttcg
    10801 gaaagctgta ccagcccttt gagcagctgt ttttgcttca gcggatcttt caggcggata
    10861 cgacggaaca gttctggtgc gaagttcgga ataccggtga acttcatcat ctcaccctgg
    10921 gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca ggccgaggat atcgcccgga
    10981 tacgcttctt caacgtgcga acggtcaccc gccataaagg tcagcgcgtc ggagatcacc
    11041 acatctttcg cagtgcgcac ctggcgcagc ttcatgcctt tttcatattt accggacacc
    11101 acacgcataa acgccacgcg gtcgcggtgt ttcgggtcca tgttggcctg aattttaaat
    11161 acgaagccgg taaatttatc ttcgctcgct tctacggtac gggtatcggt ctgacgcggc
    11221 atcggcgcag gcgcccactc caccaggcca tccagcatat gatcaacgcc gaagttaccc
    11281 agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa ggaacagctc tttgtcgaac
    11341 tcgttagacg cgcctttcac cagttccagt tcgtcacgca gctgctgtgc cagatcttca
    11401 ccaaccgcag catcgagatc cgggttattc agccctttaa caatgcggac ttcctgaatg
    11461 gtgtgccctt taccgctctg atagagatag gtttcatctt tataaaggtg gtaaacgcct
    11521 ttaaacagct tgccgcagcc aattggccag gtgatcggtg cgcagccaat tttcagctcg
    11581 ttctcaactt catcgagcaa ttccatcggg tcgcggatat cacggtcaag tttgttcata
    11641 aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca tcagcttacg ggtacgatct
    11701 tcaacacctt ttgcggcgtc gataaccatc aggcagcagt ccaccgccgt cagggtacga
    11761 taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg
    11821 tgatacggaa actgcatcac agacgtagta atggagatcc cacgctgctt ttccatctcc
    11881 atccagtccg acttagcatg ctggttggaa ccacggcctt ttactgtacc ggcggtctga
    11941 atggcctgtc cgaacagcag caccttctcg gtgatggtag tcttaccggc gtccgggtga
    12001 gaaataatgg caaaagtgcg gcgcttcgcc acctcttgca aataaggaga caacgtcata
    12061 attctttctt cttgagtaag cggcatcgcg ccgcgcttgt tgaaaacgaa aaattgcggc
    12121 tattttaccc atcaacgggg gggaggcaat cagtgtttac acaggagctg ctccagttcg
    12181 tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg gttgctcgcg atggtgtgca
    12241 ttcagccagc aggtcgcaag cccggcgttg atgccaccga gaatatcgga ctcggcagtg
    12301 tcgccaacca tcagcacgcg tgaacggtca ggattgcccg cctgttccag cgcataatcg
    12361 aaaattttct tattcggttt ggcaacgcca acttcttcgg aaatcaccag caaatcgaag
    12421 taatcacgca ggcccgtgcg ttccagacgc acctgttgca aggcactaaa gccgttggtg
    12481 atgatgccga ttttggcgtt gccacgaatg gcgttaagca gagaaaccgc gcccggcagc
    12541 ggcgtgcaga tttccgccat cgcattaata aaggcttcgt tgagtttacc tggctcgacg
    12601 ttcagccgtt cggcccagct ctcaaaccgc ccgtgctgaa gctgtaatga agtgatcgcg
    12661 ccgttttgat aatccaccca cagtggcttg ttaacggcct gatagtcctg aaaatcttca
    12721 gcggtaaagg tgacgctgta atcaagaaac atccgctgca ggccggtgaa tgagtcaaag
    12781 gtaaacagcg tttcatcggc atcaaagaaa atccagtccc acttcattat tccaccttgt
    12841 cttacatact gattggcaac gccatgatga tggcgtcttc gcgaccgtcc gtggtggggt
    12901 agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact ttcgtacagg gcaatggcgg
    12961 cagcatttga ggcacggact tccagccata gtgtcgccac gccgcgtttt tccagttcgt
    13021 cgatcagatg ttccagcagc gcccttccca atccctgacg ctgatagtca ggatcgaccg
    13081 caatattgaa caatgtagct tcatccagca ccacttgcgt aatcgcaaac gccgccattt
    13141 tgccgttttg cgttaactga aagttgagat aacgctcgcc ctggttgctg gcaaaggttt
    13201 tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg gtaagccgct ggtaaatcag
    13261 tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt gttgccataa cgcggcgcgt
    13321 gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg ccacctgagc gccttccagt
    13381 gatagcggtt cgtcagtacc caaccgccag ctgttgcagc gactgccttg cggcagcatc
    13441 gcgatttttt ctggcgtcag ttgcagcact tgatcggggc tgacggttaa tgcgcgcaga
    13501 acatcgctca ctaaaggatc agtcagggcg ggaagatcgt ttgccaccat caccagacgg
    13561 acgtgtgccg ggatcgcaat ggcaatctcc ccctgcaacg cgccagggcg acgcagcgac
    13621 cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc gggatgtcat agcgaatcgc
    13681 tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata ccgcgccaac atatggctat
    13741 aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc atttaccccg gcaagtgaag
    13801 tcttgctgcg tcacagtgat gatttcgaac aaagccgtat tctgtttgcc ggagacttac
    13861 aggatgacct gcccgcgcgt ttagataccg cggccagccg tgctcatacc cagcaattcc
    13921 agcattggca ggtgttaagc cgccagatgg gggataacgc ccgttttagt ctggtcgcca
    13981 cggcggatga tgtcgcagat tgcgatacgc tgatttacta ctggccgaag aacaaaccgg
    14041 aagcccagtt ccagttgatg aatttacttt ctctgcttcc ggtggggacg gatatttttg
    14101 ttgttggcga gaaccgcagc ggcgtgcgca gcgccgagca gatgctggct gattatgcgc
    14161 cgttgaataa agtcgacagc gcccgtcgct gtggcctcta ttttggtcgt ctggaaaaac
    14221 agccggtatt tgatgccgat aaattctggg gcgaatacag cgtcgatggc ctgacggtca
    14281 aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt cggtagccag ttgctgctct
    14341 cgacgttaac cccgcacacg aaaggtaaag tgctggatgt cggctgtggc gcgggcgtgc
    14401 tttcggttgc ctttgcgcgc cattcgccga aaattcgtct caccttgtgc gatgtctctg
    14461 cgccagcggt agaagccagc cgcgcaacac ttgcggccaa cggtgttgaa ggtgaagtct
    14521 ttgccagcaa cgtcttttcc gaggtgaaag gtcgttttga tatgatcatc tccaacccgc
    14581 cgttccacga tgggatgcaa accagcctgg atgcggcgca aacgctgatt cgcggtgcgg
    14641 tgcgtcatct taatagcggc ggcgagctgc gaattgtagc gaacgccttc ctgccttatc
    14701 cggacgtgct ggatgagaca tttggcttcc acgaagtgat cgcgcaaaca gggcgcttca
    14761 aggtgtatcg cgccattatg acccgccagg cgaagaaagg ttaattatct cgccgaatac
    14821 cgtgtcggat gcggcgtgaa cgccttatcc gacttacaaa acctgcacgt tagtctcctg
    14881 atggacattt ttccagcaat tacacatcag gcgataatta actattgacg aaaagctgaa
    14941 aaccactaga atgcgcctcc gtggtagcaa ttctttttaa gaattgatgg tatgcgaagg
    15001 tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt ccttacggac gtgggggttc
    15061 aagtcccccc cctcgcacca attatcttta cttcctttct tgtttcttcc ctgttttttc
    15121 atccctgatt caaattcatt atcatcagca tgatgccagc aataagcgcc aggcacgatg
    15181 gtagcaaaat gtaatgccgc aactgtttgt gatacagcat caccggcgtc aatactaatc
    15241 ccatcagaat gacaatccgc cactgatccg gcgataaatc cgcatacatc agccctgcaa
    15301 tcagaaatcc cggcagcaac actccccagc cactgcccag cgtacgttgc aacatggttt
    15361 tatctctttc attgataatg ataaccaata tcatatgata atttttatca cttgcaagcc
    15421 agagaaatcc cttgctatcg ggtaaaccta tcgctaggat tagcaatcat tatcatttag
    15481 attactatcc cgattatggc ctatcgttcc gcaccgctct atgaagatat catctggcga
    15541 acgcatctcc agccacagga tgctaggctt gcacaagccg tacgtgcgac gatcgccgaa
    15601 catcgtgaac atttgctgga gtttatccgc ctggatgaac ccgctccgct taacgccatg
    15661 acgctggcgc aatggtcatc accgaatgcg ctaagttctc tactggcggt ctattccgat
    15721 catatctatc gcaaccaacc aacgatgatc cgcgagtaca aaccgctgat ctcactatgg
    15781 gcacaatggt atatcggcct gatggtgcca ccattaatgc tggcgctact gacgcaggaa
    15841 aaagcattag atgtgtcgcc ggaacatttc catgctgagt ttcacgaaac cggacgcgtc
    15901 gcctgtttct gggtcgatgt gtgtgaagat aaaaacgcaa caccacattc gccgcagcag
    15961 cgaatggaaa cgttaatcag ccaggcgctg gttccggttg tgcaggcact agaagcgacc
    16021 ggagagatca acggcaaact tatctggagt aataccggtt atttgatcaa ctggtatctt
    16081 actgagatga agcaactgct cggcgaggcg acggttgaat cgctgcgcca tgccctcttt
    16141 tttgagaaaa cgcttactac cggtgaagat aatccactct ggcgtaccgt ggtgctacgc
    16201 gacggcctgc tggtgcgccg cacttgctgc cagcgttatc gcttaccgga tgtacagcaa
    16261 tgtggcgatt gtacgctgaa atgaaagcac tgccggggaa gtaaacccgg catcatgcgg
    16321 attaataggg atgcaacaca ttacttgttt cgcaatgccg catacataac agaatatctg
    16381 cggcctccaa caaccctgcg tcagaactga cgcccagttt cgacatcaca ttaaatttat
    16441 gtgcacggat cgttttgata ttgcgtttaa gctgctcggc aatttgtgtc attgagtagc
    16501 cacgcgacat aaagcgcaat atttctctct ccgtcgggct taacgtccgg ctttggttaa
    16561 tgtaccactg attgttcagt cggtcggtcg cctgacgtac accatttaat gacaaaaaga
    16621 gttcctgatg aaaaatctcc agcgttgacg ctttacttaa tacaccgtcc agcggtgatg
    16681 gcgacaatga accaatcagc cgagcttcaa tatcatcatc cgcaataact aaacgccggg
    16741 tacgcggaaa cttaatcgcc agttcagtca ggcaagataa tccctctctg cgctcacttc
    16801 tcatggcaga aaaagaaaaa atgaccgccg caaacgagat ccgcgacatg gcatcctgaa
    16861 atgactcctg actggtatag gtatggagct tataagggtt gcccgcaaac tttctgaata
    16921 aactctcaat accgatacta ctcatgacgc atttttctat gagtgcgaca tttctcttct
    16981 taattcggct gtgttccatt ctgcggcatc tccataaaca gaaggcaagt taagttctct
    17041 tgcaccctgc gcgctattta tccatgcata catttcagcg ttgctgtgaa ttgccagtcg
    17101 acgcatcgca ctgttttttt gggcgctgat agttttatta cttttcttaa gcaatgaagc
    17161 tatctggttg atgccccatc ctttacctaa tagccgcagt accttgcgct ctgaaagagt
    17221 gagaatgacg gagcgatagc taaaatcatg agtttcagtc attgccggag aggtcagcat
    17281 ggcgctgata cgctctgcgt gcgtattgcc ggaacgtacg gtcttgacca gattctcaat
    17341 gggttcaaca tcagacaata acgtcgccgt agggcacatg agcagttcta cagcctggga
    17401 ataccaagag cgtgacacca tgaaaaccca gtgaatttcc cgatattgtg agattaaaga
    17461 ataataatgt tcacaaacag aacgtggatc ttcactttca ccggctaaat cagcaatgac
    17521 taatccggaa cgacgtaatt gtaataaggt aaggtcctcc gcagaggcgc tggatattat
    17581 ttcatattca gggaagtgag tcctcatgac ctcttttaac cctgcttgca taacaggtat
    17641 tttactgata acaattccat ttttgcagca tcctggcaac atatttcctc cacatccact
    17701 gtctaaaaat tatgatggtt tattcaggat attcattcct taaatgtttc cagcaaatta
    17761 atagattaag cgaataaatt atccttagca ttcactttca tatcagcata ctcctgctgc
    17821 acaacaactc cactgcatgt gtcagaaggt tagcatcaaa tgataacctg gtgaaatagc
    17881 ctggagacat ttaaaatatc tacattaagt tgttagaaat acttaatgaa aaacagaaat
    17941 ctcattcatt taatttataa ttaaatgata tctcttcccg cagaatgcgg gaatcacaca
    18001 atcaatattt attcaataaa atctgatgat tttaacttgt taacactaaa aaacgcgccc
    18061 agctatctat tatcctgacc cgtaaaggct aaaacaataa attcattata gtgataatat
    18121 tcatgtgata gtttgtttgc ggcgagagat aattcgcttt ttatcaccga gcttgtcccg
    18181 ataaccagga ttaatcagcg caattttgat cttaacctac cgcaaaacaa ccttgcttca
    18241 tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag
    18301 ttatgcaaac tgagcaacag cgagccgtaa cacggctttg tatccagtgt ggattatttc
    18361 ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg
    18421 cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg ctgacaacta
    18481 ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc
    18541 atgtggtgac tgaagtccag cacattgtga ttcttgcgga gcatcatctg ctggattaca
    18601 aaggcgtaga gaaacgattt agccaaattc agccattacg ttacccaaga tggctggtag
    18661 ccttaatggt tggcctttct tgcgcctgtt tctgtaaact caataacggt ggctgggatg
    18721 gtgccgtcat caccttcttt gccagtacga ccgcgatgta tatccgccag ctgctggcac
    18781 aacgtcatct tcatccacag atcaactttt gccttaccgc tttcgccgcc accaccattt
    18841 ccggattgct tttgcaacac cccactttca gcaatacccc caccattgcg atggccgcca
    18901 gcgttctgct gctagtgccg ggctttccgt tgattaatgc cgtcgccgat atgtttaaag
    18961 gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgaca ctggctacct
    19021 gcgtcggcgt agtgatggca ctgacgattt gggggctacg cggatgggtg tgatcgaatt
    19081 tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg gctttgcgat
    19141 ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg ctgcttggct cgataggtca
    19201 tggttcccga atgatcttga tgaccagcgg gttgaatatt gagtggtcaa cctttatggc
    19261 ctccatgctg gtcggcacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa
    19321 agtgtttacc gtggcggccg ttatccctat gttcccgggc atatcggctt ataccgcaat
    19381 gatttcggcg gtaaaaatca gccagttagg ttacagcgaa ccgttgatga ttaccctgtt
    19441 aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc
    19501 tggattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcgctt aagcacacgg
    19561 atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc ttctcgttca
    19621 tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac gtcgttggta
    19681 ttgacgccct ggtacacgat caccaaaccg ttctggcaaa agctgaaggc ggtgtggttg
    19741 ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc ctggctgaac
    19801 tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga tgtcgatctg gacgatcaac
    19861 tctatcagga accgcaagcc gctcccgttg ctgtacccat ggggaaattc gccatgtatc
    19921 cggactggca acccgatgcc gactttatcc gcctggcggc tctatggggc gtggcgctaa
    19981 gagagccggt aaccaccgaa gaactggcct cattcattgc ctactggcag gcggaaggta
    20041 aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa atcggtcgtg
    20101 ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagtgaacct gacagccaaa
    20161 ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc aacgcctgca
    20221 aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagagc tactggcctg
    20281 gcagaaagaa caaggggcga tccgctccgc cgctctcgaa cgtgaaaatc gggcgatgaa
    20341 aatgcagcgc acctttaacc gctccggtat tcgcccactg catcagaact gctcctttga
    20401 gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc gccagtatgt
    20461 cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag gaaccggcaa
    20521 aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat ccgtattgat
    20581 catcaccgtg gccgatatta tgtcggcgat gaaagatacc ttcaggaata gcggtaccag
    20641 cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg atgagatcgg
    20701 cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg atcgccgctc
    20761 ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga tgaccaagtt
    20821 gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg tgatcttcaa
    20881 ctgggatagc taccgtagcc gtgtaacagg taaagagtat taatccccgt ttctggcggt
    20941 atactgcgac acaattccac cttgagtaat gatgatgaaa actgttaaac atcttctgtg
    21001 ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt cctggaaaga
    21061 tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac aggaaggcgg
    21121 ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga aaccaggcct taagcgcaga
    21181 taacatgaac aatgccgcag gcattctgca atactgcgcg aagcaaaagc tggcttcggt
    21241 aaccgatgtc gaaaacatca agaaccaggt gctggaaaag ctgggcctga acagtgaaga
    21301 gcaaaaagaa gacaccaact atctggacgg cattcagggt ttgctgaaaa ccaaagatgg
    21361 tcagcaactc aatctggata acatcggaac aactccgctg gcagaaaagg tgaaaaccaa
    21421 agcctgcgat ctggtgttaa agcaggggct gaacttcatt tcctgaaccg cgaaacgccg
    21481 cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg ctcctggacg
    21541 gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta taaactaatt
    21601 cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac gccattacat
    21661 tgtgcttctc aaaaagctta aacgttggct aaacggcccg gtttagtgag gatcgcccgt
    21721 tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac gcatggaaag
    21781 cgggacgtaa cacctggtgg tttgcagcca cattaacggt gctggggcta tttgtcgttt
    21841 taaatatcac cctgtttgcc agcgactatt ttactggcga tggtattaac gacgcggttc
    21901 tctatacctt aaccaacagc ctgaccggtg ctggcgtcag caaatacatt ttgccgggta
    21961 tcggcattgt gctggggctg acagcggtgt tcggtgcgct gggctggatc ctgcgccgtc
    22021 gtcgccatca tccgcaccat tttggttaca gcctgctggc gctcttactg gcgctgggtt
    22081 cggtggacgc cagcccggca tttcgtcaga taacggaact ggtgaaatcc cagtcacgcg
    22141 acggcgaccc ggactttgcg gcttattata aagagccgtc gaaaactatc cctgacccga
    22201 aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt gataacgagg
    22261 ctttcccgga cctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg gatttcagcc
    22321 acacgcagca gctgccggga acagattaca cgattgcggg catggtggct tctcagtgcg
    22381 gcataccgct gtttgccccc tttgaaggca acgcctccgc ttctgtttcc agcttcttcc
    22441 cgcagaacat ctgtctgggc gatatcctga aaaactcggg ttatcagaac tatttcgtgc
    22501 agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct gaagtcgcat ggcttcgacc
    22561 acttatacgg ctcagaagag ctgaaaagcg tggtagccga cccgcactat cgcaacgact
    22621 ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa gagctttccc
    22681 gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac ccggatggtt
    22741 ttatctctcg tacctgtaac cgcaaaaaat atgattttga cggtaaaccg aatcagtcat
    22801 tcagcgcggt aagttgcagc caggagaata tcgcgacgtt tatcaacaaa atcaaagcgt
    22861 caccgtggtt taaagatact gtcatcgtcg tctcttctga ccatttagcg atgaacaaca
    22921 cggcgtggaa atacctcaat aagcaggatc gcaataacct gttttttgtc attcgtggcg
    22981 acaagccgca gcaagagacg ctggcagtga agcgtaacac gatggataac ggagcgacag
    23041 tgctggacat tctcggtggc gataactatc tcggacttgg tcgtagcagt ttatccgggc
    23101 agtcaatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg aagccggata
    23161 tcatccgtct gtggaaattc cctaaagaga tgaaagagtt caccatcgac cagcagaaaa
    23221 acatgattgc cttctcgggt agccatttcc gtttgccgct gctgttgcgg gtttcagaca
    23281 aacgcgtgga accgctgcca gaaagcgaat actcagcacc gctgcgtttc cagctggccg
    23341 atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg ctacaagatg gcacaactct
    23401 gggctccgga actggcactt tccaccgact ggtgtgtctc gcaggggcaa cttggcggtc
    23461 agcaaattgt tcagcatgtt gacaaaacaa catggaagag caagacggca tttaaagata
    23521 cggtcatcga catggcgcgt tacaaaggca atgtcgatac gctgaagatt gttgataacg
    23581 atattcgcta caaagccgac agtttcatct ttaacgtcgc cggtgcgcca gaagaggtga
    23641 aacagtttag cgggatttcc cgtccggagt cgtggggccg ctggtccaac gcgcagctgg
    23701 gcgatgaagt aaaaatcgag tacaagcatc cgctgccgaa gaaatttgac ctggtgatta
    23761 ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta ggcaatgaag
    23821 aacaaactct tgtgctgggc aatgaagtga ccaccaccac actgcatttc gataacccaa
    23881 ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac gaagggaata
    23941 tcctcggaca ctcgccgcgt aagctcggga tcggcatggt ggaaattaaa gtggtagaac
    24001 gtgaagggta attcctgact gatgtgtagg tcgcataggg tgcgacctac acaccttctc
    24061 cggcttacaa cacattatta agaaacaaaa aaaacagcgc ccaacacttc cccggacgct
    24121 gcatggcaac tttaataatc tcgcctttca gaaagtaatt acgcctttaa ttaactcacg
    24181 attgttaatc acatcgcgct cgtaggtttc tgccagcgtg gcgaacggat agcgatgggt
    24241 taacatcatg ccagcagtga tttttccttc cgccatcagt cgaccgactt tagcaaaatc
    24301 ttccggcgtg gcgttgcggc tgcccatcat cgtcgtttct tttttatgga attccggatc
    24361 ggagaactgc aactcacctt taaacaggcc gacaaatacc accgtgccgc cgtgacgaat
    24421 cagattcacg gtgttattca tcgcatgttg attacctgtc gcgtcgatca ctttctgcgc
    24481 cagcgaacca ccaaactgcg cccgcagctg cgcgtcaaaa tcttcggctg acgggtccag
    24541 cacgggcaac tccagacgcg ttgccacatg ttcacggcgc gccggactgg tatccgccac
    24601 caccacctgt gcgccatcgg ctttagcgat tgccgccgcg cccagaccga ttggcccggc
    24661 cccgaccacc agcacctgct cgccgggagc aatggctgcg cgacgcaccg catgagcgct
    24721 aatagcgaaa ggttcaatca atgcggccgc ctgcgggtca ataccgtctg cgggcaaaat
    24781 gttcgccacc ggcacgctca aatactcact aaaaccgcca tcctgatgca cgccaatgac
    24841 tgaaattttt tcacagcaat tggtacgccc gcttttacac gccgggcatt gctgacaggc
    24901 aacataaggg atcacagcaa cttgctgacc atttttaaga tcagcaatat ttttacccag
    24961 cccaacaatc tccccacata tttcatggcc taaaacccgt ggataactaa aaaatggttg
    25021 atttccaccc caggcatgaa tatcggtacc gcaaatcccg acagacttaa tttttattaa
    25081 tgcttcattg tcacccggaa taggtatctc gcgttgtttc cagactaatt ctttcggctg
    25141 ctggcaaatt aaaacattca tcgtagacat gattcggctc tccatttttt gttgccttag
    25201 atatcaatga aaatgaaaaa tggcagcgga acggaaaatc tgttttgtga aaacacacgc
    25261 ataaaaatgt tttaaatcgg gttttaatgg actcacaagg aggaaaatat gagtcgttca
    25321 caaaatttac gccacaatgt gattaaccag gtcattgatg atatggcgcg gggtcatatc
    25381 ccttccccac ttccatcgca aagtgcgctg gcggaaatgt acaacatcag ccgcactaca
    25441 gtgcgtcata ttctcagcca cttacgcgaa tgcggcgtct taacgcaggt tggaaacgac
    25501 tatgttattg cgcgtaaacc tgaccatgat gacggtttcg cctgtactac cgcttcaatg
    25561 agtgaacaaa acaaagtgtt tgagcaggct tttttcacga tgataaacca gcgccagtta
    25621 cgcccagggg aaactttttc tgaactgcaa ctggctcggg ccgcgggtgt cagcccggtg
    25681 gttgtaagag aatatctttt aaaattcggc cgttataatc ttattcaaag cgaaaagcgc
    25741 ggccagtgga gcatgaagca attcgatcaa tcctacgctg agcagctgtt tgagctacgc
    25801 gaaatgctcg aaacacattc acttcagcac tttctcaacc tgcctgatca tgatccccgc
    25861 tggctgcagg cgaaaaccat gctcgaacgt catcggctac tgcgtgacaa catcggcaat
    25921 agctttcgca tgttctcgca acttgacaga gattttcact ccttactgct ttcagctgcc
    25981 gacaatatct tttttgatca atcgcttgag atcatctcag tcatctttca ttttcactat
    26041 caatgggacg aaagcgatct taaacagcgc aatatcattg ccgtcgatga gcatatgacc
    26101 attctcagcg ctctgatttg ccgcagtgac cttgatgcca cccttgcgct ccgtaaccat
    26161 ttgaattcag cgaaacaatc aatgattcga tccattaatg agaacacgcg ttatgctcat
    26221 taagtacgga ttaaaaacag caaactatta gcgggtgcac ggttttatca aaagataaat
    26281 accgggaaat tcccgcttac ctatgctcac aatcagtcga ctaaaaatgt ttttgtgagc
    26341 ggtagtaaag tcctaaaact ttaacctgcc gttaatgtca accttgcgta atgcacaatt
    26401 acgtaaacaa ttaaaattat aaatacagga gtccggcgtg gaaaaagaaa atatcaccct
    26461 cgatccgcgt tcttcattta ctccatcttc gtcggcagat attcccgtgc caccagatgg
    26521 attagttcaa cgcagtaccc gaattaaacg cattcaaacc accgccatgt tgttattatt
    26581 ttttgcggcg gtaatcaatt atctcgaccg cagttcgctg tcggtagcaa atttaacgat
    26641 tcgtgaagaa ttgggattaa gtgccaccga aatcggcgct ttgctctccg tgttttcact
    26701 cgcttacggc attgcgcaac ttccttgcgg cccactattg gatcgtaaag gcccacgcct
    26761 gatgctggga ctggggatgt tcttctggtc actgttccag gccatgtctg gcatggtgca
    26821 cagctttacg cagttcgtgt tggtgcgtat cggtatgggg attggtgaag cgccgatgaa
    26881 cccatgcggt gtaaaagtca ttaacgactg gttcaacatc aaagagcgcg gacgcccgat
    26941 gggcttcttc aacgcagctt ctaccattgg cgttgccgta agcccaccga ttctggcggc
    27001 gatgatgctg gtgatgggct ggcgcgggat gtttattacc attggtgtac tggggatttt
    27061 tctcgccatc ggctggtata tgctctatcg caaccgcgag cacgtagaac tgactgccgt
    27121 tgaacaagct tatctcaatg caggtagcgt caatgcccgc cgagatccgc tcagttttgc
    27181 cgaatggcgc agcctgttcc gtaaccgcac aatgtgggga atgatgctcg gattcagtgg
    27241 catcaactac actgcgtggc tgtatctggc ctggcttcct ggttacctgc aaacagccta
    27301 taacctggat ttaaaaagca cagggttgat ggcggctatc cctttcctgt ttggtgctgc
    27361 cgggatgctg gtcaacggtt acgttactga ctggctggtc aaagggggaa tggctccgat
    27421 taaaagccgt aagatctgca ttattgccgg gatgttctgt tctgccgcct ttacgctgat
    27481 agtaccacaa gcgacaacat ccatgatggc ggttctgctg attggcatgg cactgttctg
    27541 tattcacttt gccggaacat cctgctgggg cttgatccac gtcgcagttg cttctcgcat
    27601 gactgcgtcg gtgggcagta tccagaactt tgccagcttc atctgcgcct ctttcgcgcc
    27661 gatcattact ggttttattg ttgatacaac ccactcattc cgtctggcac taatcatttg
    27721 cggttgcgtc accgcagcgg gagcactggc gtacatcttc ctggttcgtc agccgatcaa
    27781 cgacccgcgt aaagattaaa ctccctatgc ccgataacat tttgcttatc gggcattttt
    27841 atggcgatta aaacgtttcc cagttctccc cgctatctgc cactgccatt ttacgcggcg
    27901 tagctggcgt cacggttttt accacagccg atgtttcacg ctgctgttgc tgctgttgct
    27961 gaatccggaa cactgccaca gcttcggtca ggcgactggc ctgctcttcc aacgcggcgg
    28021 cggcagcggc agactcttcc accagcgcag cgttctgttg agttacccgg tccatctcag
    28081 caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca gaagctattt
    28141 cgcccataat gtccgtcacg cgggtcacag cactgacaat ctccgccatt gtttccccgg
    28201 cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca atcaggcttt
    28261 taatttcacg agccgcctgg gcgctacgct gggccagatt acgcacttct cccgcaacca
    28321 ccgcaaaacc acgtccttgc tcacccgcac gcgctgcttc aaccgccgcg ttcaaagcca
    28381 gaatattggt ctggaaggca atgccgtcaa ttacgctgat aatatcggcg attttctgcg
    28441 aactggtgga gatatcacgc atagtctgca ccacgttatc taccactttg ccgccgcgtt
    28501 gcgccgtttc agaagcactt aacgccaggt ggctggcctg gcgcgcattc tcggcgttct
    28561 gtttcaccgt tgcggtgagt tgctccatgc tggctgccgt ctcttccagc gaagcggcct
    28621 gttgctcggt gcgcgaagag agatcgttat tgccggtagc gatttcgctg gcaccgctat
    28681 agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct ccctgcatat
    28741 ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca atcggtttca
    28801 ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt ggcgctacca
    28861 gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg atcatcacgc
    28921 ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg gcgatatcat
    28981 agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg tcctgatatc
    29041 cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt tggatcagct
    29101 ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct gcggcggtgc
    29161 tctgacgcgg gtcacgcggc aacgcttcgt aatctgccca gtttttttcc gcctgtttca
    29221 acgaaatact ggcactctgc atcagctcag caacggttga accgctacca atattattct
    29281 gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc aacaacgcga
    29341 cccagctgcc attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca gtgaaatttt
    29401 ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt aaaaggccaa
    29461 aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac atggtttctc
    29521 tttcctgtgg accgatgcaa aattttcggc ctgaaatgag aaaactttat aggtagcgac
    29581 ttgaaactgg ttaattttac ctcacttttt ttaatttttg ttcaaaaata caataaccac
    29641 ttattgatac attttctttt cttatttttt tcacacgaat tgtatctatt ttgaacgaaa
    29701 tattttatag agtgtggaat tttaagaaag gacatctgaa atgttaaacc gtgacttcga
    29761 tcatctctca acgctattac atctcttttt tgaacgtgag gatatttgcc aaaagcttaa
    29821 tattattgac caaaacaata tcaccggttg ggaagtgtgg ttccaggtag agtttgctaa
    29881 tatgctctgc tcaacagacc atgaatggtg gcgtgaacaa gcactttcct gtgatatgcg
    29941 caaaaaacct gagcgcccga cactacgcac agatttctta ctacgtaaaa aaggctgggc
    30001 acaagatagc tatatcgcgc ttgaaataaa acaaaacaga gacgcgacca gttgtgttaa
    30061 aaatatgatc gcagacttag aaaaaagcgc aaaaataaaa cgttccgaac tcgatttacg
    30121 cagtttctgg accttgggta tcacccctat gatagacagg gaaaggctgg actctctcat
    30181 cgattgctat ttagatgaga aattttatct tactaaatct cgtaaaaaac acgttttact
    30241 ta
//
LOCUS       DAZFDC010000050        29957 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFDC010000050 DAZFDC010000000
VERSION     DAZFDC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.050"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            126..1709
                     /gene="yegH"
                     /locus_tag="ACHJ48_002325"
     CDS             126..1709
                     /gene="yegH"
                     /locus_tag="ACHJ48_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO4707879.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGELLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1784..2122
                     /locus_tag="ACHJ48_002326"
     CDS             1784..2122
                     /locus_tag="ACHJ48_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4707880.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2112..2402
                     /locus_tag="ACHJ48_002327"
     CDS             2112..2402
                     /locus_tag="ACHJ48_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4707881.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2455..4308)
                     /gene="asmA"
                     /locus_tag="ACHJ48_002328"
     CDS             complement(2455..4308)
                     /gene="asmA"
                     /locus_tag="ACHJ48_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO4707882.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESRKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4330..4911)
                     /gene="dcd"
                     /locus_tag="ACHJ48_002329"
     CDS             complement(4330..4911)
                     /gene="dcd"
                     /locus_tag="ACHJ48_002329"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO4707883.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5003..5644)
                     /gene="udk"
                     /locus_tag="ACHJ48_002330"
     CDS             complement(5003..5644)
                     /gene="udk"
                     /locus_tag="ACHJ48_002330"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO4707884.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5962..9280
                     /gene="dgcE"
                     /locus_tag="ACHJ48_002331"
                     /pseudo
     CDS             5962..9280
                     /gene="dgcE"
                     /locus_tag="ACHJ48_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9381..10229)
                     /gene="alkA"
                     /locus_tag="ACHJ48_002332"
     CDS             complement(9381..10229)
                     /gene="alkA"
                     /locus_tag="ACHJ48_002332"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO4707885.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSADLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10363..11715
                     /gene="yegD"
                     /locus_tag="ACHJ48_002333"
     CDS             10363..11715
                     /gene="yegD"
                     /locus_tag="ACHJ48_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4707886.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARIQEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11728..13668)
                     /gene="yegI"
                     /locus_tag="ACHJ48_002334"
     CDS             complement(11728..13668)
                     /gene="yegI"
                     /locus_tag="ACHJ48_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO4707887.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13665..14426)
                     /gene="pphC"
                     /locus_tag="ACHJ48_002335"
     CDS             complement(13665..14426)
                     /gene="pphC"
                     /locus_tag="ACHJ48_002335"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO4707888.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAINEAMAYVSQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDVVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLTLALWLP"
     gene            complement(14423..15082)
                     /gene="yegL"
                     /locus_tag="ACHJ48_002336"
     CDS             complement(14423..15082)
                     /gene="yegL"
                     /locus_tag="ACHJ48_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4707889.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADIKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15303..15362)
                     /gene="ibsA"
                     /locus_tag="ACHJ48_002337"
     CDS             complement(15303..15362)
                     /gene="ibsA"
                     /locus_tag="ACHJ48_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO4707890.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15634..15690)
                     /locus_tag="ACHJ48_002338"
     CDS             complement(15634..15690)
                     /locus_tag="ACHJ48_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4707891.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15962..16018)
                     /locus_tag="ACHJ48_002339"
     CDS             complement(15962..16018)
                     /locus_tag="ACHJ48_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO4707892.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16298..17545
                     /gene="mdtA"
                     /locus_tag="ACHJ48_002340"
     CDS             16298..17545
                     /gene="mdtA"
                     /locus_tag="ACHJ48_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO4707893.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17545..20667
                     /gene="mdtB"
                     /locus_tag="ACHJ48_002341"
     CDS             17545..20667
                     /gene="mdtB"
                     /locus_tag="ACHJ48_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO4707894.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQISDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20668..23745
                     /gene="mdtC"
                     /locus_tag="ACHJ48_002342"
     CDS             20668..23745
                     /gene="mdtC"
                     /locus_tag="ACHJ48_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO4707895.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGMVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKSKQTVTE"
     gene            23746..25161
                     /gene="mdtD"
                     /locus_tag="ACHJ48_002343"
     CDS             23746..25161
                     /gene="mdtD"
                     /locus_tag="ACHJ48_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4707896.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSSTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25158..26561
                     /gene="baeS"
                     /locus_tag="ACHJ48_002344"
     CDS             25158..26561
                     /gene="baeS"
                     /locus_tag="ACHJ48_002344"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO4707897.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26558..27280
                     /gene="baeR"
                     /locus_tag="ACHJ48_002345"
     CDS             26558..27280
                     /gene="baeR"
                     /locus_tag="ACHJ48_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO4707898.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27471..27803
                     /gene="yegP"
                     /locus_tag="ACHJ48_002346"
     CDS             27471..27803
                     /gene="yegP"
                     /locus_tag="ACHJ48_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO4707899.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27951..29312
                     /gene="yegQ"
                     /locus_tag="ACHJ48_002347"
     CDS             27951..29312
                     /gene="yegQ"
                     /locus_tag="ACHJ48_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO4707900.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29642..>29957)
                     /locus_tag="ACHJ48_002348"
     CDS             complement(29642..>29957)
                     /locus_tag="ACHJ48_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4707901.1"
                     /translation="KKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDNQ
                     YYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKC
                     GE"
BASE COUNT         6928 a         7990 c         7902 g         7137 t
ORIGIN      
        1 taatgtgagg ttattaccct caaataatat gagataaata gtgctgcaac attgcatttt
       61 tgccccgatt tatccatgat cgaattgtga catttgtcat acaacgaata ggttttgtac
      121 ttactatgga atggattgcc gatccgtcta tctgggccgg gttaatcacg ctgattgtga
      181 tcgaactggt cctcggcatt gataacctgg tctttattgc catcctcgcc gaaaaactac
      241 cgccgaagca gcgcgaccgc gcacgggtta ccgggctgtt gctggcgatg ttaatgcgcc
      301 tgttactgct ggcgtcaatc tcctggctgg tcaccctgac tcaaccgctg ttcagcttcc
      361 gctcgtttac ctttagcgcc cgcgacttaa tcatgctgtt tggtggtttc ttcctgttgt
      421 tcaaagccac gatggagctg aacgaacggc tggaagggaa agacagcaat aatcccacac
      481 aacgcaaagg tgcgaagttc tggggggtgg tgacacaaat tgtggtactg gacgccatct
      541 tctcactcga ctcagtgatt accgccgtcg ggatggtcga ccatttactg gtcatgatgg
      601 ccgccgtggt tatcgctatc agcctgatgt tgatggccag caagccgtta acgcaatttg
      661 ttaacagtca cccgaccatc gttattctct gcttaagctt cctgttgatg attggcttta
      721 gcctggtggc agaaggtttc ggcttcgtca ttccgaaagg ctacctgtac gctgccattg
      781 gtttctcggt gatgatcgag gcgctcaatc agttggctat ctttaaccgg cgacgttttc
      841 tttccgctaa ccagacactg cgccagcgga ctaccgaagc ggtaatgcgc ctgcttagcg
      901 ggcaaaaaga ggatgcggaa ctggacgccg aaaccgcgtc catgctggtg gatcatggta
      961 accagcagat ctttaatccg caggaacggc ggatgattga gcgggtactt aatcttaacc
     1021 agcgtaccgt cagcagcatt atgacgtcgc gccacgatat tgagcatatc gatctcaacg
     1081 cgccggaaga cgagatccgc cagttgctgg agcgaaatca gcatacgcgg ctggtggtta
     1141 ctgatggcga tgacgcggaa gatttgctcg gtgttgttca cgttatcgac ctgttacaac
     1201 aatcactgcg cggcgaactg ctcaacctgc gggtgttaat tcgccagccg ctggtgttcc
     1261 cggaaacctt gccgttgtta cctgccctgg agcagttccg taatgcccgc acacactttg
     1321 cttttgtggt ggatgagttt ggctcggtgg aagggattgt gacattaagt gacgtcactg
     1381 aaaccattgc cggtaactta ccgaacgaag tggaagagat cgacgcccgc catgatattc
     1441 agaagaatgc ggacggttcc tggacggcga atggtcatat gccgctggaa gatctggtgc
     1501 aatatgtgcc gctgccgctg gatgaaaaac gtgaatatca caccattgcc gggctattga
     1561 tggaatattt gcagcgtatt ccaaaacctg gcgaagaagt tcaggtgggg gattatttgc
     1621 ttaaaacgtt gcaggtggaa agccatcgcg tgcagaaggt gcagattata ccgctgcgta
     1681 aggatggcga gatggagtac gaggtgtgat aaaacatcaa atgcgcaggt tcaaactgcg
     1741 catttgattt atacaaaatt tagtataatc taaggatgga gtaatgagaa aaaagctggc
     1801 ttttcttgat accagtcttg acgatttacg cgcatttcca gaatcgtctc gacaggaaat
     1861 tggctatcaa ctcgacagga tccaacaagg gttaaaccca tacgactgga aacctttttc
     1921 aactatcggc cctggtgtgc gagagattcg tactcgcgat gctgatggga tctatcgcgt
     1981 catgtatgtc gcaaaatttg aagaagcggt ttacgtattg cattgttttc aaaagaaaac
     2041 acagactacc agtcaatcgg atattgattt agccaaacga cgctataaag aacttgttca
     2101 ggagcggaaa aatgaaaatt gaaacctttg atagtgtgtg ggatgcggtg agtgacaccc
     2161 ctgaacaagc ggaaaatatg cggatccgtg ccgagcttgt aacgatcatt aataactgga
     2221 ttgaacaaca agggtttagc caggctcagg cagcttctgc tttaggggtc actcaacctc
     2281 gcatctctga acttgccaga ggtaaaatcc agatctttag tatcgataaa ttgatcacca
     2341 tgatggctca tgcaggacta catattcaac gtattgaagt tcaatatcca catgctgctt
     2401 agtgctataa atacagatgg cgacaaattg atgccgccat ctatataact tgtcttacat
     2461 cttctccagc aacttcttca catctttgcc attgcgggaa tctttattcc gctcggccca
     2521 gtcattcagg cgacgtttcg cttcgtcctg tagatgtttg cgcagtaact gatccacttg
     2581 cagactgtaa ttgagttgct gccagttgcc ataaacccgc agcggtactg gcgtttcttt
     2641 gaggaaatca atcagtttgc tttccccgtt ccagccaccc acgacccgaa tatcaaactg
     2701 ggtgtcgcag gtttgatctg ccagattcaa catgccttcc cctgtaagcg ccagcacagg
     2761 cgattgacct tgcatgtcgt ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt
     2821 aaagcggtca agacgcgtta cgttatcgaa gttttcagcg gccttcacat caccaccatt
     2881 acgttctacc gcttgctgaa tcatctgctg gaagttcatc ccttccatgc gcgtatcggc
     2941 catttcgaca tgtgcctgtc cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc
     3001 accggagaaa tcaccagcca atgacatttt tccggtcaac gaaatcggat agttaaacgc
     3061 cttcagaatg gtgccaatct caacgttttc cagccgtggc tggaagttta tccgcggatt
     3121 tattgatgtc gcgtccagcg tgcccggcag tgaaacctgt ccaccgttaa gcttgccctg
     3181 tagttgagta atttccagca aacccgactt gttggtcatt tgcgtggcaa catctgtaaa
     3241 attcattccg cgccagcgca cgttactggc ctgcaacaaa atatcagccg taaagccttg
     3301 cagtccctga taggctggtt catcaatacg cgaagaaata accgggcgcg gcaacgttga
     3361 ttggctctgc ccctgctgcg cggcaccgtt ttcaccattc gcggtttcat taagcgggat
     3421 gaggttgtca agattcagtt gcgggaattg cagcctcagc tgccattccg gtttctctgt
     3481 cagcgtgacc tgtgcttgcc cgctcagcgt actatcattg gcggtcaaac taatttggtt
     3541 aaatgaaagg cgtttacgtg actcctgcca ctgggcctgg aaactcccct gcccctgaat
     3601 accttgtttt ggtaaatcgg caccctgcaa ctgccagtta atttgttcaa tagccgccgt
     3661 taaatcatgc ggataatcag aagcatctac cgtaccgtta agggatatcg tgagatcgcg
     3721 ctgatcgcga ttaacccgcc cggagaactc aaatgagcca cgatgttggg gatcttgttc
     3781 catttgcagg cggatattgc ggattgtcac ctgctcgtca tcttcatgct ggaacaccag
     3841 cacgctgtcc gccaccttaa gactggatat atcaaacgac catccgcgat cgtctgacag
     3901 atccggcaag gtattgtcgc gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt
     3961 ctgcggcgtc agttggatca ctgccccttt tagcatcacc tgcttaacgc tcagttgatg
     4021 actcagtagt ggtaaaagcg ccacgtccag gcgcatgttg tcggcgcgaa ccagtggctg
     4081 gctcgcgccc tgggcggtga gagacattcg cccggagagg atactaagct gcggccagac
     4141 gtgccaacgc agtggcccgt cgagctgcaa ttgataaccg ctacgtgcag caacttgctt
     4201 gaccatatag tcgcggaaat cattcggatt caccagcaac actaacgcag ataacccggc
     4261 caccagcacg accaggagta tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat
     4321 gggcctgaat tagtctttat cgattcggct ggctaccgcg ccctgctggt tgcgatactt
     4381 cgcatcttca cggcggttgt aaggtcgcgc cgccgggccg gaaagcggct caaagctcag
     4441 cgcaccaatt aacatgcccg gacgcagcgc cagcggcagc ttaccggagt tgtagaactc
     4501 cagcacaatg caaccagacc agcccggatc gatacggtgc gcggtgacgt gcaccatcag
     4561 ccccagacgc gccagtgagg aacgcccgtc cagccagccc accagatcgg ctggcagcgt
     4621 caccgactcc agcgtcaccg ccagcgccag ctctcctggg tgaagataga acgcctcgct
     4681 ttcatcgaga acgatctcat cgctcatcac gcggtcaagc gcggcgctca cttcatcttt
     4741 gggaccgctc agatcgataa acgctgccgt gtgaccacgg aaggtacgaa atttattgcc
     4801 caggcgtaca tccaccgtcg cgccgttaat acgctccact ggcggacgtg ggttgatcga
     4861 caaacggcct tcatcaagcc aggcttcaat atctcggtca cacagacgca tggcattttc
     4921 tcctttcgcg catcactccc ttaacgccaa ttacgtcaag ggcataccag gttatcactg
     4981 aacggtacac aatttatcaa gcttattcaa agaactgact tattttcgct ttcaatatat
     5041 cgatcgcgat gcggtttttc ccgccgcgcg gcacgataat gtccgcatat tgtttagaag
     5101 gctcaatgaa ttgcaggaac atcgggcgca cggttttttg atattgcgcc atcacggaat
     5161 ccattgaacg cccacgctcg ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca
     5221 gcggggtatc aacgaaaatg gagaagttaa gttcgtcacg caaacgtgca tccgtcagca
     5281 acaaaatgcc ttcgagaatg atgaccttct tcggctcaac cgtcaccgtt tctttcatac
     5341 gcgtatgttc aacatagctg taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt
     5401 gtaaatgctc aagcagcagg ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa
     5461 cgcgttcttc catcgacaga tggctttgat ctttgtaata gcagtcttcg ggaattacgc
     5521 cgatgtgttc atcaccgact tgctcacgta attcacgata aagggtactg gcaataagac
     5581 tcttgccgga agccgatgcg ccagcgatac cgataatgac gcactgatga gactgatcag
     5641 tcatatattt agcgacctga ttaacctgga tgttaggaag ggggcgacga agcgccaaac
     5701 gcggcaatta tagggatttc atccgcctga taccagtcga atagcgttgc cgcgcgctca
     5761 gagttaattg ttgacgaaga attcccggtg gcaaattacg ttgatcagtt ttatacaagg
     5821 taaaaattgt tatacgcagt tgcgcaaatt atccgccttt acgtcacttt atgagcaatt
     5881 cgcatataaa atgtaaaact tttgtactag cataaacaca gaaacgaata ctggcgacct
     5941 ggccttgcgg ataaagcggt aatgagcaaa caatcacagc atgtattaat tgccctgccc
     6001 cacccgctgc ttcacctggt cagtttaggt ttagtctcgt ttatcttcac ccttttctcg
     6061 cttgagcttt cgcagtttgg cacccaactc gccccactgt ggttcccgac gtccatcatg
     6121 atggtggcgt tttatcgcca tgccgggcgc atgtggccag ggattgcgct gagctgctcg
     6181 ctgggaaata tcgccgcatc catcctgctt ttttccacca gctcgctgaa catgacctgc
     6241 acgaccatca atattgttga agccgtggtc ggggcagtgc tgctacgtaa attgctgccg
     6301 tggtataacc ccttgcaaaa tctggctgac tggctgcgtc tggcactcgg cagcgccatt
     6361 gttccacctc tgttgggggg tgttctggtt gtcctgctga cgcccggaga cgatcctcat
     6421 cagggcattt ttgatatggg tactgtcaga atccatcggc gctctggcac tggtgccgct
     6481 gggattgtta tttaaaccac actatctgct gcgccatcgc aacccacggt tgctttttga
     6541 gtcgctgctc acgttagcca tcacactgac gttaagctgg ctttcgatgc tgtatctgcc
     6601 gtggcctttt actttcatta ttgtgctgtt gatgtggagc gccgtgcgcc tgccacgaat
     6661 ggaagccttt ttgatcttcc ttaccacggt gatgatggtg tcgctgatga tggccgcgga
     6721 tccctccctg cttgctacgc cgcgtacgta cctgatgagc catatgccgt ggctaccgtt
     6781 tttgctgatc ctgctgcccg ccaacatcat gacgatggtg atgtatgcct ttcgtgcgga
     6841 acgcaaacac atttccgaaa gcgaaacccg ttttcggaac gccatggaat attccgccat
     6901 cggcatggca ttagtgggca ccgagggaca atggctgcaa tccaacaaag cactctgcca
     6961 gtttctcggt tacagtcagg aagagctgcg cggactcacc tttcagcaac tgacctggcc
     7021 ggaggatctc aataaagatc tccaacaggt tgaaaagctg ataagcggtg aaataaacac
     7081 ctattcaatg gaaaaacgtt actacaaccg caatggcgat gttgtctggg cgttgcttgc
     7141 cgtctctctg gtgcgccaca cggatggcac gccgctctat tttatcgctc agattgaaga
     7201 cattaacgag ctaaaacgca ccgaacaggt gaatcagcaa ctgatggaac gcatcacgct
     7261 ggctaacgaa gcgggcggga ttggcatctg ggagtgggaa ctgaagccga atatttttag
     7321 ctgggataag cggatgttcg agctgtatga aattcctccg catatcaaac cgaactggca
     7381 ggtgtggtac gagtgcgtgc tgccggaaga tcgccaacac gccgaaaaag tgattcgtga
     7441 ttcgttgcaa tcacgctcgc cctttaaact ggaatttcgc attgccgtga aagatggcat
     7501 ccgccatatc cgcgccctcg ccaaccgggt actgaataaa gaaggcgaag tcgaacgcct
     7561 gctcggcatt aatatggata tgactgaggt taaacagctt aacgaggcat tgtttcagga
     7621 aaaagagcgc ctgcacatta cgctggactc catcggcgaa gccgtggtct gtattgatat
     7681 ggcgatgaaa attaccttta tgaatccagt ggcggagaag atgagcggct ggacgcagga
     7741 agaagcgtta ggtgttccgc tcctgacggt gttgcatatt acttttggcg acaacggacc
     7801 attaatggag aacatttaca gtgccgacac ctcacgttcc gcgatcgaac aagatgtggt
     7861 gttgcactgt cggagcggcg gcagctacga cgtgcattac agtattacgc cgttaagtac
     7921 tctggacggc agcaatatag gctcggtact ggtgattcag gacgttaccg aatcacgcaa
     7981 aatgctgcgc cagctgagct acagcgcctc ccatgatgca ctgacgcatc tcgccaaccg
     8041 cgccagtttt gagaaacaac tgcgtatcct gctgcaaacg gtaaacagta cgcatcagcg
     8101 acatgcactg gtgtttatcg atcttgatcg ctttaaagcg gtgaatgaca gcgccgggca
     8161 tgcggcgggc gacgctttgc tgcgcgaact ggcgtcgttg atgctgagta tgctgcgctc
     8221 cagtgacgtg ctggcgcggc tcggcggtga tgaatttggt ctgctgctgc cagactgcaa
     8281 tgttgaaagc gctcgtttta tcgctacacg tattatcagt gctgtgaatg actatcactt
     8341 tatatgggaa ggccgggtac atcgggtagg tgccagcgcc gggattacct tgatcgatga
     8401 caacaatcat caggcggcag aagtgatgtc gcaggctgat atcgcctgtt atgcctccaa
     8461 aaatggtggc cggggccggg tgacggttta cgaaccgcag caagctgccg cacatagcga
     8521 acgggcggcg atgtcgcttg atgaacagtg gcggatgatt aaagagaatc agttgatgat
     8581 gctcgcccac ggtgtcgctt cgccacggat cccggaagcg cgtaatttgt ggctgatttc
     8641 acttaagctc tggagttgcg aaggcgagat tattgatgaa caaacatttc gtcgtagctt
     8701 cagcgatccg gcgcttagcc atgctcttga ccgccgggta ttccacgaat ttctccagca
     8761 ggccgcaaaa gcggttgcca gtaaaggcat aagcatcgcc ctcccccttt ccgttgccgg
     8821 tttgagtagc gccacgctgg tgaatgatct gcttgagcag ctggaaaata gccctctacc
     8881 accacggtta ttacatctga ttattccggc agaagcgatt ttcgatcacg cagaaagcgt
     8941 gcaaaaactg cggctggggg gatgccggat cgtattcagt caggtgggcc gcgatctgca
     9001 aatcttcaac tcgctgaaag cgaatatggc agattacctg ctacttgatg gtgagttatg
     9061 cgccaacgtg cagggtaatt tgatggatga gatgctgatt acgattattc aggggcacgc
     9121 tcagcgactc gggatgaaaa ccatcgccgg gccagtcgtt ttacccttag tgatggatac
     9181 gctttctggc atcggcgtcg atctgattta tggtgaggtg attgccgatg cccaaccgct
     9241 ggatttgctg atgaatagta gttatttcgc gattaactga gggagcgttt gcccattgcc
     9301 tgatgcgacg ctaacgcgtc ttatcaggcc tacaaatcgc tcattccgca aggcgctcgc
     9361 accgcatccg gcgaccaacg tcatgcttcg tctggttgcc agccttccgt ataccagata
     9421 tgcaacagcg cataagaacg ccagggcttc cagcgctcgg cataacggcg gatttgcgcc
     9481 ggtgtcattc ccggaaatcg ctgtttaatc agataatcat ccggcaggaa aacatctttc
     9541 gcctgccagc cacgcaaagc aaaataattc gccgtccagc gcccgatacc cggaaaagtt
     9601 tgcagcgttt tcatcgcctg ctccacatcg cccggtattg tcattggtaa ggtgccctcc
     9661 agcgccgcat ttgccagatg aatcagcgcc tctgcccgtt tcaacggcat acctaacgct
     9721 tttaatgcct gcgggtctgc tgctgccagc cgctgaggcg tggggaagca gatatactcc
     9781 ggaaaatcat ccagccgttc gccataaagc tgtaccactt tggcagtcaa ttttgccgcc
     9841 atcgccacgc tcaccagttg gcctaaaatc gcccgcacgc cttgctcaaa agcatcaaca
     9901 ctgccgggta aacgcaatcc gggccgcgcc gcgcctaact tgcccaacgc accgttaaca
     9961 atctgcgggt tacattgcag atcaagcagg cggctcattt tcgccagaca ctcagcggca
    10021 acgggttcta aatctgcact taaatttatg tgcagagtat ggcgggctat atccggaata
    10081 gcagtcacca cgccgcgata ttcgcccacc gccagactac gggcataata atcgtcagcg
    10141 accgtttcca caccactcac tgcacgggcg gcgagaaatc ccaacatcca cgaccagtca
    10201 tacggcggct gccagttcag ggtatacatc gcatctcctt attcatccgc tttcagcata
    10261 aacgttattc agacgctgcg ctttgctttc atattccggt tgtcgcgacg gcaacatttc
    10321 gctaaagtca cgccccttct tcaccggcat ggggattatt tcgtgtttat tggttttgat
    10381 tacggtacag caaactgttc agtagcggtc atgcgtgacg gtaaaccgca cttgctaaaa
    10441 atggaaaacg acagcacgct gctgccttca atgctttgcg cgccaacgcg tgaagcggta
    10501 agcgaatggc tgtaccgcca tcatgatgtt ccggcagacg acgatgaaac gcaggcactg
    10561 ttgcgtcggg cgattcgtta taatcgcgaa gaagatatcg atgttacggc gaaaagcgtg
    10621 cagttcggtc tttcctcact ggcacagtac attgatgatc cagaagaagt gtggtttgtg
    10681 aaatcaccaa aatcgttcct cggtgccagc ggcttaaaac cgcagcaggt agcgctgttt
    10741 gaggatctgg tctgcgcaat gatgttgcac attcgccagc aggcgcaagc gcagctgcca
    10801 gaagcgatta ctcaggcggt gattggtcgc ccgatcaact tccaggggct gggcggcgat
    10861 gaagcaaacg cccaggcgca agggattctg gaacgcgcgg cgaagcgtgc cggattcagg
    10921 gacgtggtat tccagtacga gccggtagcg gctgggctgg attacgaagc caccttacag
    10981 gaagaaaaac gggtactggt ggtggatatc ggcggtggta cgactgactg ttcattgctg
    11041 ctgatggggc cgcagtggcg ttcgcgtctc gatcgtgaag ccagcctgct gggtcacagt
    11101 ggttgccgta ttggcggtaa cgatctggat atcgcactgg cgtttaaaaa cctgatgcca
    11161 ctgctgggta tgggtggcga aaccgaaaaa ggcatcgccc tgccgattct gccgtggtgg
    11221 aatgcggttg ccatcaacga cgtacctgcg cagagtgatt tctacagtag tgccaacggt
    11281 cgtctgctta acgatctggt acgcgatgcc cgcgaaccgg aaaaagtggc cctgctacag
    11341 aaagtctggc gtcagcgttt aagctatcgc ctggtgcgta gcgcagaaga gtgcaaaatt
    11401 gcgctttcaa gcgtagcgga aacccgcgcc tcactgccgt ttatcagcga tgaactggct
    11461 acgctgatta gccagcaagg gctggaaagc gccctcagcc agccactggc gcggattcag
    11521 gaacaggtgc aactggcgct ggataacgcc caggaaaaac cggacgttat ctacctgacc
    11581 ggcggaagcg cccgttctcc gctgattaaa aaagcgctgg cagaacagtt gccgggcatt
    11641 ccgattgcag gcggcgatga ctttggctcc gtcaccgccg ggctggcacg ctgggcggaa
    11701 gtggtgtttc gttaattgaa aaatggctca gcagcggctt aaatctgcct gcgcctgagc
    11761 cagtttttct gccgcctgac gtaacggttc catcaacggc atggtccgtg ctggagcatg
    11821 aaggcggaat cgctgcaact ctgtcgcgcc gacagtcaac gccgattcca gccgatgtcg
    11881 tttggcggcc attttcgcca ttaccgcttg tctttctgca ggcgttaccg cttcgttcgg
    11941 cctgaacaca aaacggcgtt cacagctcgc tttccagtcg ataaccgcct gggtcagatg
    12001 atcaccaaac cctttaactt gcttaacccc acgacgggta acatccgcgg ctgtttcaat
    12061 accaaaagac cgtaacgccg ctttacgcgc agggccaacg ccgggaatag aggcagcatc
    12121 aataaaaaat ccctccagaa acttctgctt ctgccgttcc cttgcagtgt catgaagtgc
    12181 tgccagagcg cgtttctcct cttccggtaa cccgagaatt tcgtccttca ttttttcgag
    12241 catcgcccgt ttggcgataa aaccttccag cccgcccaac tgttggatct ggctgactaa
    12301 atggtcatag tccattttcg cgcgattaaa tgcctcacgg cgttgctgga tttctgcttt
    12361 ataaactttg cttgtcagac tgccaataat ccagatagcc gccagcacag gaaccaaaat
    12421 aatataacgc ggttctgcct gaaggccgca aagcaacagc gataacgctg acagtgcgat
    12481 ctcaatcaga atgatgtatt cgcgccgtaa caggcctaaa ggaagcggcc tgccagtcgg
    12541 ttggaaatga tcgggtaatg gcaattgcaa tgctggcggt gctactgacg ccatcaccat
    12601 cgcccagact ttcgccagca caaaatcacc actggtggta atgacctctt cgccgagatc
    12661 aataaaatag ataacgcctt gattatccag cgcacaccac gggcagtcag tcaaatgacc
    12721 ggggtaaaca tgcattgccg aaacggtaca tttctttaac tgttggcgta gtaaatccag
    12781 tgccgctacc cacgcttttg ccgtcggacg cgcggttgcc acgccacttt ccgtgaatgc
    12841 ctgctgaaac atggcttcaa catcgcccgg taacatcgac agcggaatcg atcgtggcgg
    12901 cggttttaaa ccacgtcgct ggttgtctga cgcgtaggca taacggaaat gggcaatatc
    12961 cgtctccagc gcattacccg catcagagat aagcggcaca ccagaataag gatgccgccc
    13021 accaaacaag acgtgaaaaa tcagcaacgc aaggccaaaa ttatcgtgat tcgcggtacg
    13081 ttcaaaaccg acaaatgatg gcaaggtttg tagctctggc ggcgtaaaat gcgacacgcc
    13141 gacttcgcat aaatgcagtg tgccattggc gttaatctga aaggagtcac tatcgatcaa
    13201 caccactttg ctgtcgcgac ctaccataaa gctgttctgg tttacgtccc ccacgacgtg
    13261 cccgtgctcg tgaaccgtag caaaagatga agcaatattg cgcgcaacat agagtagaaa
    13321 atcccacgca caatgaggat aactctggcg acgatgtgcc gggctataga tcatatgaat
    13381 cggttcttta ccagaaactt ttggcatcat aaaaccgata acttttccgc ctcgtccacc
    13441 gtgaagcgtt gcctgcggcc aggcgacata attcaacaac tgcgcgtcag ctgtcgcagc
    13501 cataaaggca agtttgtcct gttttaaggc gggtggcggc gtgtgataaa tcttggcgac
    13561 gctatcgaca aactcattga tatcataaac cgcgccttcg ccacctttgc ccagttcacg
    13621 gcctggcgta acgcactcac cagtagcagt atataaagtg ggtttcatgg taaccacagc
    13681 gccagtgtca acgttttatc atcatcggta cgctcgttta ccgctgggct cgacaaaaac
    13741 tgctttaaca attcaggtaa taaatcgagt tgttcctgcg ttgctgccgc cagtccattg
    13801 aaaaacgggg taaagaaagg cacatgcgga gaattatcca gcatatttaa cgccagtcgc
    13861 tgaataccat ccgtaaatgc tgcaacttta tgcgcacgct cagtgctggt gaaagtttcc
    13921 agtctggaaa cgacatcttc atcggtaatg aaatgcgtca tattggcata ttcacccacc
    13981 atcggcgtaa gcggtaattg cagaccgtga ccaaaatcca ccaccacgcc gccatcgcca
    14041 atctgcataa tcaaggtgcc gttagccgat gaaatcaatc ccagaaacgt gcaggcaaaa
    14101 tcgcgaactg ctaattcttt agcttccgcc tcggcaaaaa gtcgctgacg aacggtcagc
    14161 acaatatccg tggcgagaat atcattgagt ccaaattcac cgtgctgcac tttttgcgac
    14221 acataagcca tcgcttcatt gatggcaagc atcgcgcctt cgccaccctg cgagacactg
    14281 cctgcgccat cagcaaggaa catcaccagt aatggctgct ggtcatttag ccaggcaacc
    14341 tgcatctggc aggcatcctg acaaggtaaa tcagcgctaa tgtgcgacgt tccaacagcg
    14401 gatgcataaa ccagacgcca gctcacactg acgtccagcc ttttggcgct tccagcacaa
    14461 cttccgttcc cggcgtggag cgggaaaccg aacgcagcga actggataac cagctaaaca
    14521 gttcacggaa ttgtaatccc tgcagtggca aaggctgacg aacgctgatt tgtgccagag
    14581 tcttaatatc cgccccctga acaccgatgg aaaagaaggc aaacttctta tcttcttccc
    14641 cctgaaacac tttgttggcg gcagcctgcc actcatcggt tggtgcacca tcggtgatca
    14701 ggaaaatcca cgggcgataa tacgaaatac catttgcacg atactcacgt ttccgctcct
    14761 ctaccatatc cagggctttg gtaatggcgg cacccattgg tgtatccccc tgggcaaaca
    14821 ggatgggcgg gaaaaaatta gcggcgctgg taaagggttg ttccacatgc accgggccga
    14881 acgtcacaat accaagttcc actcttttta aggccagcgg gtcggcaagc agctcatcgc
    14941 gaaaggtaac caatccggcg ttaagttcat taatgggtct accgttcatc gaaccagaaa
    15001 catccagcag caaaatgcag gggcaacgtg gttccgggtt actggcaaaa tcgctggtgg
    15061 caaacgtgat ttgttcgctc ataagaaatc ctttcttaga gaattattga ggtccggtat
    15121 cataccgatt gcaataatga aataagagca aacgaagaaa ctttcagaat taggcccggc
    15181 ggcgcagaaa attttgctat tcgcctcgca agaacggaga gccgtcgatt gacactaagg
    15241 gcggagtgac ataatttcag gagtgagggt tagggaaagg tttccccctc cccctggtgt
    15301 tcttagtaag cctggaagct aatcactaag agtatcacca gtatgatgac gtgcttcatc
    15361 ataacccttt tcttatttaa ggccccttcc tcgggagggg ctttcccgtt tcagcatccc
    15421 gctgaaatcg tcggcttacc tcctttcgcc atgcatgcag tctatcgctg acttgcggat
    15481 aaaatagttt ccagtgtgat tattgcgtaa cgtctcgcaa agaatatttg ctggcaggat
    15541 cgcagactgc aaagcctgca gattgacaat cttatcgtga aggcatactt tcaggagtga
    15601 gggtagagcg gggtttcccc cgccctggta gtcttagtaa gccgggaagc ttatgactaa
    15661 gagcaccacg atgatgatcc acttcatcat gaccctttcc ttatttaagg ccccttcctc
    15721 gggaggggct ttcccgtttc agcgtcccgc tgaaatcgtc ggcttacctc ctttcgccct
    15781 gaattcagtc tatcgctgac ttgcggataa aatagtttcc tctgtgatta ttgcgtaacg
    15841 tctcgcaaag aatatttgct ggcaggatcg cagactgcaa agcctgcgga ttgacattct
    15901 gaacgtgaag gcatactttc aggagtgagg gtagagcggg gtttcccccg ccctggtagt
    15961 cttagtatgc tgggtagcta atcactaaga gcaccacgat gatgagtatc ttcatcatga
    16021 ccctttcctt atttatggcc ccttcctcgg gagggggctt tcccgtttca gcgtcccgct
    16081 gaaatcgtcg gcttacctcc tttcgccatg caagcagtct atcgctaacg cgtagataaa
    16141 atagtttcct gtgttattac tggatgcgtg ctcgcaaatg tgcccgccat tcagacgatt
    16201 cccgatagtg tttcataatt cctccatttt tctcccttat tggctggcta cactagtatc
    16261 attccgcgaa acgtttcagg aagagaaact cttaacgatg aaaggcagtt ataaatcccg
    16321 ttgggtaatc gtaatcgtgg tagttatcgc cgccatcgcc gcattctggt tctggcaagg
    16381 ccgcaatgac tcccagagtg cagccccagg ggcgacgaaa caagcgcagc aatcgccagc
    16441 gggtggtcgc cgtggtatgc gttccggccc attagccccg gttcaggcgg cgaccgccgt
    16501 agaacaggca gttccgcgtt acctcaccgg gcttggcacc attaccgctg ctaatactgt
    16561 tacggtgcgt agccgcgtgg acggtcaact gatggcgtta catttccagg aaggccagca
    16621 ggtcaaagct ggcgatttac tggcagaaat tgaccccagc cagttcaaag ttgcattagc
    16681 acaagcccag ggccaactgg caaaagataa agccacgctt accaacgccc gccgtgatct
    16741 ggcgcgttat cagcaactgg caaaaaccaa tctcgtctcc cgccaggagc tggatgccca
    16801 acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct gatgaagcaa gcgtcgccag
    16861 cgcgcagctg caactcgact ggagccgtat taccgcacca gtcgatggtc gcgttggtct
    16921 caagcaggtt gatgttggta accaaatctc cagtggtgat accaccggga tcgtggtgat
    16981 cacccagacg catcctatcg atttgctctt taccctgccg gaaagcgata tcgctaccgt
    17041 cgtgcaggcg caaaaagccg gaaaaccgct ggtggtagaa gcctgggatc gcaccaactc
    17101 gaagaaatta agtgaaggca cgctgttaag tctcgataac caaatcgatg ccactaccgg
    17161 tacgattaaa gtgaaagcac gctttaataa tcaggatgat gcgctgtttc ccaatcagtt
    17221 tgttaacgcg cgcatgttag tcgacaccga acaaaacgcc gtagtgatcc caacagccgc
    17281 cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg aatagcgaaa acaaggtcag
    17341 caaacatctg gtgacgccgg gcattcagga cagtcagaaa gtggtgatcc gcgcaggtat
    17401 ttctgcgggc gatcgcgtgg tgacagacgg cattgatcgc ctgaccgaag gggcgaaagt
    17461 ggaagtggtg gaagcccaga gcgccaccac tccggaagag aaagccacca gccgcgaata
    17521 cgcgaaaaaa ggagctcgct cctgatgcaa gtgttacccc cgagcagcac aggcggcccg
    17581 tcgcgcctgt ttattatgcg tcctgtggcc accacgctgc tgatggtggc gatcttactc
    17641 gccgggatta tcggttatcg cgccctgccc gtttcggcgc tgccggaagt ggactatccg
    17701 accattcagg tggtcacgct ctacccaggt gcgagcccgg atgtcatgac ctctgccgtt
    17761 accgcgccgc tcgaacgcca gttcgggcag atgtctggcc tgaaacagat gtcgtcgcaa
    17821 agttccggcg gtgcgtcagt tatcacttta cagttccagc taacattacc gctggatgtc
    17881 gccgagcagg aagtgcaggc cgcgattaac gctgcgacca acttgttgcc gagcgatctg
    17941 cctaacccgc cggtttacag caaagtgaac ccggcagatc cgccgatcat gacgctcgcc
    18001 gtcacctcaa cagccatgcc gatgacgcaa gtggaagata tggtggaaac ccgcgtcgcg
    18061 cagaaaatct cgcagatttc cggcgtcggc ctggtgacgc tttccggcgg ccagcgtccg
    18121 gctgttcgcg tcaaacttaa cgctcaggcg attgccgccc tcggcctgac cagcgaaacc
    18181 gtgcgcaccg ccattaccgg cgctaacgtt aactcggcta aaggtagcct tgacggccct
    18241 tcccgtgcgg ttacgctttc cgctaacgac cagatgcaat ccgccgaaga gtatcgccag
    18301 ctaatcatcg cctaccagaa cggcgcgccg attcgtctgg gcgatgtcgc aactgtagag
    18361 caaggtgcag aaaacagctg gctcggcgca tgggcgaata aagaacaggc cattgtgatg
    18421 aatgttcagc gccagcccgg tgctaacatt atctccaccg ccgacagcat ccggcagatg
    18481 ctgccacagc tcaccgagag tctgccgaaa tcggtgaagg tgacggtact ttctgaccgt
    18541 accaccaata tccgcgcatc cgtcgatgat actcagtttg aattgatgat ggctatcgcg
    18601 ctggtagtca tgattatcta cctgtttttg cgcaatattc cggcgaccat cattcccggc
    18661 gttgcagtgc cgctgtcgtt aatcggcacc ttcgcggtca tggtgtttct cgatttttca
    18721 atcaataacc tgacgctgat ggcgttaact atcgccaccg ggttcgtggt cgatgacgcc
    18781 atcgtggtga tcgaaaatat ttcccgctat atcgaaaaag gcgaaaaacc gttggcggcg
    18841 gcgctcaaag gcgcaggtga aatcggcttt accattatct cgctgacctt ctcgctgatt
    18901 gcggtgttga tcccactgct gtttatgggc gatatcgtcg ggcgactgtt ccgcgaattt
    18961 gctattaccc tggcggtagc gattttaatc tcagccgtgg tgtcgctaac cctgacaccg
    19021 atgatgtgcg cacggatgct cagccaggag tcgttgcgta aacagaaccg cttctcccgt
    19081 gcctcggaaa aaatgttcga caggataatc gccgcctatg gtcgtggact ggcgaaagtg
    19141 ctgaatcatc cgtggctgac cttaagcgtg gcgctcagca cgctgctgct tagcgttcta
    19201 ttgtgggtgt tcattccgaa aggtttcttc ccggtacagg ataacggcat tattcagggc
    19261 actttgcagg caccgcaatc cagctccttt gccaatatgg cccagcggca acgccaggtc
    19321 gcggacgtga ttttgcagga tccggcagtg caaagtctga cctcatttgt tggcgttgat
    19381 ggcactaacc cgtcgctgaa cagtgcgcgt ttgcagatca acctcaaacc gttggatgaa
    19441 cgtgatgatc gggtgcaaaa agtcatcgcc cgtctgcaaa cggcggtgga taaagtgccg
    19501 ggcgtcgatc tcttcctgca accaacgcag gacctgacca tcgatactca ggtcagccgc
    19561 acccagtacc agtttacctt gcaggccacg tcactggatg cgctcagtac ctgggtgcca
    19621 cagttgatgg aaaaactcca gcagctgcca cagatttctg atgtctccag cgactggcag
    19681 gacaaagggc tggtggcgta tgtcaatgtt gatcgcgaca gcgccagccg tctggggatc
    19741 agcatggcgg atgtcgataa cgccctgtac aacgcgtttg gtcagcgact gatttccact
    19801 atttatactc aggctaacca gtatcgtgta gtgctggagc acaataccga aaatacccca
    19861 ggcctcgcgg cgctggatac cattcgcctg accagcagcg acggcggcgt ggtgccgcta
    19921 agctcaattg ccaaaattga gcagcgtttt gcgccgcttt ccatcaacca tctggatcag
    19981 ttcccggtaa cgaccatctc ctttaacgtg ccggataact attcgctggg cgatgcggtg
    20041 caggcaatta tggacaccga gaagacgctg aatctgccgg tggatatcac cacgcaattc
    20101 cagggcagca ccctcgcctt ccagtcggcg ctgggcagca ctgtctggct gattgtcgcg
    20161 gcggtggtgg cgatgtatat cgtgctcggc attctgtacg agagctttat tcacccgatc
    20221 accattctct cgacgctacc cacggcaggt gttggcgccc tgctggcgtt aatgattgcc
    20281 ggtagcgaac tggatgtgat tgcgattatc ggcattattt tgctgatcgg tatcgtgaag
    20341 aagaacgcca tcatgatgat cgacttcgcg ctggctgctg agcgcgagca aggcatgtcg
    20401 ccgcgcgatg caatctacca ggcttgtctg ttgcgttttc gtccgatcct gatgaccacc
    20461 ctggcggctc tgcttggcgc gctgccgctg atgttgagta ccggggtcgg cgcggaacta
    20521 cgtcgtccgt taggtatcgg catggtcggc ggcctgattg tcagccaggt gctgacgctg
    20581 ttcaccacgc cggtgattta tctgctgttt gaccgcctgg cattgtggac caaaagtcgc
    20641 tttgctcgcc atgaagagga ggcgtaagtg aagttttttg ccctcttcat ttaccgcccg
    20701 gtggcgacga ttttactgtc ggtcgccatt accctgtgcg gcatactggg tttccgtatg
    20761 ctgccggtcg ccccgctgcc gcaggtcgat tttccggtga ttatggtcag cgcctcgctg
    20821 cccggtgcgt cgccggagac aatggcgtct tccgttgcca cgccgctgga gcgctcactt
    20881 gggcgcattg ccggggtcag tgaaatgacc tccagcagtt cgctcggcag cacgcgtatt
    20941 attttgcagt ttgattttga ccgggatatc aacggtgcgg cgcgtgatgt gcaggcggcg
    21001 atcaacgccg cacaaagtct gctgcccagt gggatgccca gccgcccgac ttatcgcaaa
    21061 gcgaacccgt cggatgcgcc aattatgatc ctcacgctaa cctccgatac ttattcgcag
    21121 ggtgaactgt acgatttcgc ctcgacgcag ctggctccga cgatttcgca aatcgacggt
    21181 gtcggtgatg tcgatgtcgg cggcagctcg ctgcccgccg tgcgcgtcgg gctgaacccg
    21241 caggcgctgt ttaatcaggg cgtgtcgctg gacgacgtac gcactgccat cagcaatgcc
    21301 aacgtgcgta aaccgcaggg ggcgctggaa gatggcactc accgctggca gatccagacc
    21361 aacgatgaac tgaaaaccgc cgctgaatat cagccgctga ttattcacta caacaacggc
    21421 ggcgcggttc gtctgggcga tgtggcgacg gtgaccgact cagtgcagga tgtgcgcaac
    21481 gccgggatga ccaacgccaa accggctatt ttactgatga tccgcaaatt gccggaagcc
    21541 aatattatcc agacggtaga cagcatccgg gcaaaattac cggagttgca ggaaaccatt
    21601 ccggcggcga ttgatctgca aattgcccag gatcgctccc ccaccattcg cgcctcgctg
    21661 gaagaagtcg agcaaacgct gattatctcg gtggcgctgg tgattctggt ggtcttttta
    21721 ttcctgcgct cgggtcgcgc cactattatt cccgccgttg cggtgccggt ttcgctgatt
    21781 ggtacgtttg cggcgatgta cctgtgcgga ttcagtctca ataacctttc gttaatggcg
    21841 ctcaccatcg ctactggttt cgtggtggat gacgccatcg tggtgctgga aaacattgca
    21901 cgtcatctgg aagcgggaat gaaaccgttg caagccgcac tgcaaggtac tcgcgaagtc
    21961 ggttttacgg tgctgtcgat gagtctgtcg ctggtggcgg tgttcctgcc gctgctgttg
    22021 atgggcggat tgccgggccg actgttacgc gagtttgccg tgacgctttc tgtcgccatt
    22081 ggtatttcgt tgctggtttc tctgacatta acgccaatga tgtgtggctg gatgctgaaa
    22141 gccagcaagc cgcgcgagca aaagcgactg cgtggttttg gtcgcatgtt ggtagccctg
    22201 caacaaggct atggtaagtc actgaaatgg gtgctcaatc atacccgtct ggtgggcatg
    22261 gtgctgcttg gcaccattgc tttgaatatc tggctatata tctcgattcc gaaaaccttc
    22321 ttcccggagc aggacactgg cgtgttgatg ggcgggatac aggcggatca gagtatttcg
    22381 tttcaggcga tgcgcggtaa gttgcaggat ttcatgaaaa ttatccgtga cgatccggca
    22441 gtggataatg tcaccggctt taccggcggt tcgcgagtga acagcgggat gatgtttatc
    22501 accctcaagc cacgcgacga acgcagcgaa acagcgcagc aaattattga ccgtctacgc
    22561 gtgaagctgg cgaaagaacc gggggcgaat ctgttcctga tggcggtaca ggatattcgc
    22621 gttggcggac gtcagtcgaa cgccagctac cagtacacgt tgttatccga cgacctggcg
    22681 gcactgcgcg aatgggagcc gaaaatccgc aaaaaactgg cgacattgcc ggaactggcg
    22741 gacgtgaact ccgatcagca ggataacggc gcggagatga atctggttta cgatcgcgac
    22801 accatggcac ggctgggaat cgacgtacag gccgccaaca gcctgttaaa taacgccttc
    22861 ggtcagcggc aaatctcgac catttaccag ccgatgaacc agtataaagt ggtgatggaa
    22921 gtggatccgc gctataccca ggacatcagc gcgctggaaa aaatgttcgt aatcaataac
    22981 gaaggaaaag cgatcccgct gtcgtatttc gctaaatggc aaccggcgaa tgccccacta
    23041 tcggtgaatc atcagggatt atcggcagcc tcgaccatct cgttcaacct gccgaccgga
    23101 aaatcgctct cggacgccag tgcggcgatc gatcgcgcaa tgacccagct tggtgtgcct
    23161 tcgacggtgc gcggcagttt tgccggcacg gcgcaggtgt tccaggagac gatgaactca
    23221 caggtgatcc tgattatcgc cgccatcgcc acggtgtata tcgtgctggg tatcctttac
    23281 gagagttacg tacatccgct gacgattctc tccaccctgc cctcggcggg cgttggagcg
    23341 ctgctggcgc tggagctgtt caatgccccg ttcagcctaa tcgccctgat agggatcatg
    23401 ctattaatcg gcattgtgaa gaaaaacgcc attatgatgg tcgattttgc gcttgaagcc
    23461 caacggcacg gtaacctgac gccgcaggaa gctattttcc aggcctgtct gctgcgtttt
    23521 cgcccgatta tgatgactac cctggcggcg ctgtttggcg cgctgccgct ggtgttgtcg
    23581 ggcggtgacg gctcggagct gcggcaaccc ttggggatca ccattgtcgg cggtctggtg
    23641 atgagtcagc tcctgacgct gtataccacg ccggtggtat atctcttttt cgaccgtctg
    23701 cggctgcgtt tttcgcgtaa atctaaacaa acggtaaccg agtaaatgac agatcttccc
    23761 gacagcaccc gctggcaatt gtggattgtg gctttcggct tctttatgca gtcgctggac
    23821 accaccatcg taaacaccgc ccttccctca atggcgcaaa gcctcgggga aagtccgttg
    23881 catatgcaca tggtcattgt ctcttatgtg ctgaccgtgg cggtgatgct gcccgccagc
    23941 ggctggctgg cggacaaagt cggcgtgcgc aatattttct ttaccgctat cgtgctgttt
    24001 actctcggtt cactgttttg cgcgctttcc ggcacgctga acgaactgtt gctggcacgc
    24061 gcgttacagg gcgttggcgg tgcgatgatg gtgccggtcg gcaggttgac ggtgatgaaa
    24121 atcgtaccgc gcgagcaata tatggcggcg atgacctttg tcacgttacc cggtcaggtc
    24181 ggtccgctgc tcggtccggc gctcggcggt ctgctggtgg agtacgcgtc gtggcactgg
    24241 atctttttga tcaacattcc ggtggggatt atcggtgcga tcgccacatt gatgttaatg
    24301 ccgaactaca ccatgcagac gcggcgcttt gatctttccg gctttttatt gctggcggtt
    24361 ggcatggcgg tattaaccct ggcgctggac ggcagtaaag gtacaggttt atcgccgctg
    24421 gcgattacgg gcctggtcgc agttggtgtg gtggcactgg tgctttatct gctgcacgcc
    24481 agaaataaca accgcgctct tttcagtctg aaactgttcc gcactcgtac cttttcgctg
    24541 ggcctggcgg ggagctttgc cggacgtatc ggtagcggca tgttgccctt tatgacgccg
    24601 gttttcctgc aaattggcct tggcttctca ccgtttcatg ccggactgat gatgatcccg
    24661 atggtgcttg gcagcatggg gatgaagcgg attgttgtac aggtggtgaa tcgctttggt
    24721 tatcgtcggg tactggtagc gaccacgctg ggtctgtcgc tggtcaccct gttgtttatg
    24781 actaccgctc tgttgggctg gtactacgtt ttgccgttcg tcctgttttt acaagggatg
    24841 gtcaactcga cccgtttctc ctccatgaac accctgacgc tgaaagatct cccggacaat
    24901 ctggcgagca gcggcaacag tctgctgtcg atgattatgc aattgtcgat gagtatcggc
    24961 gtcaccatcg ccgggctgtt gctgggactt tttggttcgc agcatgtcag cgtcgacagc
    25021 agcaccacac aaaccgtctt tatgtacacc tggcttagca tggcgtttat catcgccctt
    25081 ccggcgttca tctttgccag agtgccgaac gatacgcatc aaaatgtagc tatttcgcgg
    25141 cgaaaaagga gcgcgcaatg aagttctggc gacctggtat taccggcaaa ctgtttctgg
    25201 cgattttcgc cacctgcatt gtcttgctga tcagtatgca ctgggcggtg cgtatcagtt
    25261 ttgagcgtgg ctttattgat tacatcaagc atggtaatga acagcgatta caactgttaa
    25321 gtgatgcgct tggcgagcag tatgcgcagc atggcaactg gcgcttcctg cgcaacaatg
    25381 atcgctttgt ctttcagatc ctgcgttcat ttgaacacga taattcggaa gataaacccg
    25441 gcccgggtat gccaccacac ggctggcgta ctcagttctg ggtggttgat caaaacaaca
    25501 aggtgctggt tggtccgcga gcgccgattc cacctgacgg tacacggcga cccattctgg
    25561 tcaacggtgc ggaagtgggc gcggtgattg cctcccctgt tgagcggctg acccgcaata
    25621 ctgatatcaa tttcgataaa caacagcggc aaaccagctg gctgattgtc gccctggcaa
    25681 cgttactcgc ggcactcgct acttttctgc tggcgcgcgg tttgctggca ccggtaaaac
    25741 gacttgtcga tggcacgcac aaactggcgg cgggcgattt cactacccgc gtaacgccca
    25801 ccagtgaaga tgaactgggc aaactggcgc aagacttcaa ccagctcgcc agcacgctgg
    25861 agaaaaacca gcaaatgcgg cgcgatttta tggccgatat ttctcacgaa ctgcgtacgc
    25921 cattagcggt gctgcgcggt gaactggaag ccattcagga tggcgtgcgc aaattcacgc
    25981 cggagacggt ggcgtcttta caggcggagg tcggtacact gaccaaactg gttgacgatc
    26041 ttcatcagtt gtcgatgtct gatgaaggcg ctctcgccta tcaaaaagca ccggtagatt
    26101 tgatcccact gctggaagtg gcaggcggcg catttcgcga acgatttgcc agccgtggcc
    26161 tgaaactgca attttccctg ccagacagta tcaccgtatt tggcgatcgc gaccgtttaa
    26221 tgcagttatt caataactta ctggaaaaca gcctgcgcta cactgacagc ggcggcagcc
    26281 tgaaaatctc tgccgagcag cacgacaaaa cggtgcgcct gacctttgcc gacagtgcgc
    26341 caggtgtcag tgacgatcag ctacaaaaat tgtttgaacg tttttatcgc accgaaggtt
    26401 cccgcaaccg cgccagcggc ggttccgggc tggggctggc gatttgcctg aacattgttg
    26461 aagcacataa tggccgcatt attgccgccc attcgccttt tggcggggta agcattacag
    26521 tagagttacc gctggaacgg gatttacaga gagaagtatg accgagttac caatcgacga
    26581 aaacacaccg cgtattttga tcgtggaaga tgaaccgaag ctgggacagt tgctcattga
    26641 ttatctgcgt gctgcgagct atgcgccgac gcttatcagc cacggcgatc aggtactgtc
    26701 gtacgtgcgc cagacgccgc cggatctgat cctgttggat ctgatgctcc ctggcaccga
    26761 tggcctgacg ctgtgccggg aaattcgtcg tttttctgac attcctatcg tgatggtgac
    26821 agcaaaaatc gaagagatcg accgcctgct agggctggag attggcgcag atgactacat
    26881 ttgcaagccg tacagcccac gggaagtggt agcgcgtgtc aaaaccattt tgcgccgttg
    26941 caaaccgcag cgtgagttgc agcaacagga tgctgaaagc ccgttgatta tcgacgaagg
    27001 tcgttttcag gcttcatggc gcggtaaaat gcttgacctg acgcctgcgg aatttcgtct
    27061 gctgaaaacg ctctctcatg aaccaggaaa agtgttctcc cgcgagcaat tgctcaatca
    27121 tctttatgac gactaccgcg tagtaaccga ccgtaccatc gacagccaca ttaaaaacct
    27181 gcgccgcaag ctggaatctc tcgacgccga acagtcattt atccgcgccg tttacggcgt
    27241 cggttaccgc tgggaagccg acgcctgccg catcgtttag ttttagcgac attactttgt
    27301 tagccggaga tggcattcag accagatccg gcaacattat cccacgcatg gtcagcaaac
    27361 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
    27421 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
    27481 ggtttgaact cagtaagagc agtgatagtc agttccggtt tgtgctaaaa gcgggcaatg
    27541 gtgagacgat cctcaccagc gagctgtata cctcaaaagc ctctgcggaa aagggcatcg
    27601 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagcaacg
    27661 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
    27721 atgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
    27781 agacggtgaa agacaatacg taattacgat gccgggcgtg ggttgttggc gtccggcgct
    27841 ttaccagatc ccccctacag cgctacaatg cccgccctta aagtgggggc actcccctaa
    27901 ccgcttcatc aggtgaagcg gatctgacct gtcatcagaa cgagagaatt atgtttaaac
    27961 cggaactcct ttccccggcg ggaacgctga aaaatatgcg ttacgctttc gcttatggcg
    28021 cagatgctgt ttatgcgggc cagccgcgtt actccctgcg tgtgcgcaac aacgaattca
    28081 accacgaaaa ccttcagctc ggcatcaatg aagcccacgc gctggggaaa aagttttatg
    28141 tcgtggtcaa cattgcaccg cacaacgcca agctgaaaac ctttatccgt gacctgaaac
    28201 cggtggtgga aatggggccg gatgcgctga ttatgtccga tccagggctg attatgctgg
    28261 tgcgtgagca cttccctgaa atgccgatcc atctctcggt acaggctaac gccgtgaact
    28321 gggcgacggt gaaattctgg cagcaaatgg gactgacccg cgtgatcctc tctcgcgagc
    28381 tgtcgctgga agagattgaa gagatccgca atcaggtgcc ggatatggag atcgagatct
    28441 tcgttcacgg cgcactgtgc atggcctact ccggtcgctg cctgctctct ggctatatca
    28501 acaagcgcga tccgaaccag ggtacctgca ccaacgcctg ccgctgggag tacaacgtcc
    28561 aggaagggaa agaagatgac gttggcaaca tcgtacacaa gtacgagccg attccggtgc
    28621 aaaatgttga gccgacgctg ggtatcggcg cgccaaccga caaagtgttt atgatcgaag
    28681 aagcccagcg tccgggcgag tatatgaccg cgtttgaaga tgagcacggc acttacatca
    28741 tgaactcgaa agatctgcgc gccatcgccc atgtagaacg cctgaccaaa atgggcgtgc
    28801 attcgctgaa aatcgaaggc cgtactaaat ctttctacta ttgcgcacgc accgcacagg
    28861 tttatcgtaa agctatcgat gatgccgctg cgggcaaacc gttcgatacc agcctgctgg
    28921 aaactctgga aggtctggcg catcgtggct ataccgaagg tttcctgcgt cgtcatactc
    28981 acgacgatta tcagaactac gaatacggtt attcagtttc tgaccgccag cagtttgttg
    29041 gtgagtttac cggtgagcgc aagggggacc tcgcggcggt agcggtgaaa aataaattct
    29101 ccgttggcga cagccttgag ctgatgacgc cgcaaggcaa cattaacttt acccttgagc
    29161 acatggaaaa cgccaaaggc gaagctatgc cggtagcacc aggcgatggt tatactgtgt
    29221 ggctcccggt gccgcaggat cttgagctaa attacgcgct gctgatgcgt aatttctccg
    29281 gggaaactac gcgtaacccc cacggtaagt gattaatttc gattattttt cccggatgga
    29341 aaattcttag aaaccgatca catacagctg catttattaa ggttatcatc cgtttcgctg
    29401 aaaaacataa cccataaaat gctagctgta ccaggaacca cctccttagc ctgtgtaatc
    29461 tcccttacac gggcttattt tttatgcata agccctatcc ctgataaccg tcttccattg
    29521 accacatcga tagaatctcc cttcatagca cgatgccttt cacgtaacgg catcgtgctc
    29581 gcacaggttc cggctaagca caaccagaac gcgcatgttt gacgcttacc aaaaaatatt
    29641 ctcactctcc acatttgaat gtcagacgag cgacgccatg taatcctgca ccttctgtct
    29701 tcaggtcaac tatctgcatt tttttgccct gagtaacaca gaaatgggct gcatcatttt
    29761 ttactatatt ttctgcacca gatattctgc ccctggctaa agaagcttcg gcttcggtgt
    29821 agtattggtt atcgagttta cgctgaatat tacttttata tgcaagacca aatttaccga
    29881 tacttgtctc atcattatgc acagcacaac cagacataag aaaaacacta attaatgata
    29941 tagcagctat ctttttc
//
LOCUS       DAZFDC010000051        29911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFDC010000051 DAZFDC010000000
VERSION     DAZFDC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.051"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(128..1957)
                     /gene="glmS"
                     /locus_tag="ACHJ48_002349"
     CDS             complement(128..1957)
                     /gene="glmS"
                     /locus_tag="ACHJ48_002349"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO4707902.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFAEQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2119..3489)
                     /gene="glmU"
                     /locus_tag="ACHJ48_002350"
     CDS             complement(2119..3489)
                     /gene="glmU"
                     /locus_tag="ACHJ48_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO4707903.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPAGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3841..4260)
                     /gene="atpC"
                     /locus_tag="ACHJ48_002351"
     CDS             complement(3841..4260)
                     /gene="atpC"
                     /locus_tag="ACHJ48_002351"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO4707904.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4281..5663)
                     /gene="atpD"
                     /locus_tag="ACHJ48_002352"
     CDS             complement(4281..5663)
                     /gene="atpD"
                     /locus_tag="ACHJ48_002352"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO4707905.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5690..6553)
                     /gene="atpG"
                     /locus_tag="ACHJ48_002353"
     CDS             complement(5690..6553)
                     /gene="atpG"
                     /locus_tag="ACHJ48_002353"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO4707906.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6604..8145)
                     /gene="atpA"
                     /locus_tag="ACHJ48_002354"
     CDS             complement(6604..8145)
                     /gene="atpA"
                     /locus_tag="ACHJ48_002354"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO4707907.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8158..8691)
                     /gene="atpH"
                     /locus_tag="ACHJ48_002355"
     CDS             complement(8158..8691)
                     /gene="atpH"
                     /locus_tag="ACHJ48_002355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO4707908.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8706..9176)
                     /gene="atpF"
                     /locus_tag="ACHJ48_002356"
     CDS             complement(8706..9176)
                     /gene="atpF"
                     /locus_tag="ACHJ48_002356"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO4707909.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9238..9477)
                     /gene="atpE"
                     /locus_tag="ACHJ48_002357"
     CDS             complement(9238..9477)
                     /gene="atpE"
                     /locus_tag="ACHJ48_002357"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO4707910.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9524..10339)
                     /gene="atpB"
                     /locus_tag="ACHJ48_002358"
     CDS             complement(9524..10339)
                     /gene="atpB"
                     /locus_tag="ACHJ48_002358"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO4707911.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10348..10728)
                     /gene="atpI"
                     /locus_tag="ACHJ48_002359"
     CDS             complement(10348..10728)
                     /gene="atpI"
                     /locus_tag="ACHJ48_002359"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO4707912.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11345..11968)
                     /gene="rsmG"
                     /locus_tag="ACHJ48_002360"
     CDS             complement(11345..11968)
                     /gene="rsmG"
                     /locus_tag="ACHJ48_002360"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO4707913.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12032..13921)
                     /gene="mnmG"
                     /locus_tag="ACHJ48_002361"
     CDS             complement(12032..13921)
                     /gene="mnmG"
                     /locus_tag="ACHJ48_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO4707914.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSDDKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14300..14743)
                     /gene="mioC"
                     /locus_tag="ACHJ48_002362"
     CDS             complement(14300..14743)
                     /gene="mioC"
                     /locus_tag="ACHJ48_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO4707915.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14833..15291)
                     /gene="asnC"
                     /locus_tag="ACHJ48_002363"
     CDS             complement(14833..15291)
                     /gene="asnC"
                     /locus_tag="ACHJ48_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO4707916.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15443..16435
                     /gene="asnA"
                     /locus_tag="ACHJ48_002364"
     CDS             15443..16435
                     /gene="asnA"
                     /locus_tag="ACHJ48_002364"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO4707917.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16440..17891)
                     /gene="viaA"
                     /locus_tag="ACHJ48_002365"
     CDS             complement(16440..17891)
                     /gene="viaA"
                     /locus_tag="ACHJ48_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO4707918.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17885..19381)
                     /gene="ravA"
                     /locus_tag="ACHJ48_002366"
     CDS             complement(17885..19381)
                     /gene="ravA"
                     /locus_tag="ACHJ48_002366"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO4707919.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHNMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQRCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19604..21472
                     /gene="kup"
                     /locus_tag="ACHJ48_002367"
     CDS             19604..21472
                     /gene="kup"
                     /locus_tag="ACHJ48_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO4707920.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANQEVLYALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21639..22058
                     /gene="rbsD"
                     /locus_tag="ACHJ48_002368"
     CDS             21639..22058
                     /gene="rbsD"
                     /locus_tag="ACHJ48_002368"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO4707921.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22066..23571
                     /gene="rbsA"
                     /locus_tag="ACHJ48_002369"
     CDS             22066..23571
                     /gene="rbsA"
                     /locus_tag="ACHJ48_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO4707922.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFESCDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23576..24541
                     /gene="rbsC"
                     /locus_tag="ACHJ48_002370"
     CDS             23576..24541
                     /gene="rbsC"
                     /locus_tag="ACHJ48_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO4707923.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24566..25456
                     /gene="rbsB"
                     /locus_tag="ACHJ48_002371"
     CDS             24566..25456
                     /gene="rbsB"
                     /locus_tag="ACHJ48_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO4707924.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25582..26511
                     /gene="rbsK"
                     /locus_tag="ACHJ48_002372"
     CDS             25582..26511
                     /gene="rbsK"
                     /locus_tag="ACHJ48_002372"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO4707925.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26515..27507
                     /gene="rbsR"
                     /locus_tag="ACHJ48_002373"
     CDS             26515..27507
                     /gene="rbsR"
                     /locus_tag="ACHJ48_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO4707926.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27473..28900)
                     /gene="mdtD"
                     /locus_tag="ACHJ48_002374"
     CDS             complement(27473..28900)
                     /gene="mdtD"
                     /locus_tag="ACHJ48_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO4707927.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPILGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(28923..29615)
                     /gene="yieP"
                     /locus_tag="ACHJ48_002375"
     CDS             complement(28923..29615)
                     /gene="yieP"
                     /locus_tag="ACHJ48_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO4707928.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7211 a         7665 c         7645 g         7390 t
ORIGIN      
        1 aaaacaagtg ttcaacggtg ccccaaataa tagctgaagt taattatatg gaatgaggtt
       61 agattattaa tgctggtaag taaaagggcc gccaaaaggc agcccttcgt tcagcaaatg
      121 aataatatta ctcaaccgtt accgattttg ccaggttacg cggctggtca acgtcggtgc
      181 ctttgatcag cgcgacgtga taagccagta actgcagcgg aacggtgtag aagatcggtg
      241 caatcacctc ttccacatgc ggcatctcga tgatgtgcat gttatcgcta cttacaaaac
      301 ccgcatcctg ctcggcgaag acatacaatt ggccgccacg cgcgcgaact tcttcaatgt
      361 tggatttcag tttttccagc aattcgttgt tcggcgcaac gacgataacc ggcatatcgg
      421 cgtcaatcag cgccagcgga ccgtgtttca gttcgcctgc agcgtaggct tcagcgtgaa
      481 tataagagat ctctttcagc ttcaatgcgc cttccagcgc gattgggtac tgatcgccac
      541 ggcccaggaa cagcgcgtga tgtttgtcag agaaatcttc tgccagagct tcaatgcgtt
      601 tgtcctgaga cagcatctgc tcaatacggc tcggcagcgc ctgcagacca tgcacaatgt
      661 catgttcaat ggaggcatcc agacctttca ggcgagacag cttcgccacc agcatcaaca
      721 gcacagttaa ctgagtggtg aatgctttag tggatgccac gccgatttcc gtacccgcgt
      781 tggtcatcag cgccagatcg gattcgcgca ccagagaaga acccggaacg ttacagattg
      841 ccagcgaacc aaggtaaccc agctctttcg acagacgcag gcccgccagg gtatccgcgg
      901 tttcgccaga ctgtgacaag gtgatcatca ggctgttacg acgcacggca gatttacgat
      961 agcggaattc agaggcgatt tcgacgtcgc acggaatacc tgccagcgat tcaaaccagt
     1021 aacgggaaac cataccggag ttataagaag taccacaggc gaggatctga atatgctcaa
     1081 ccttcgacag cagttcgtcg gcgttcggtc ccagctcgct taaatcaacc tgaccgtggc
     1141 tgatgcgccc ggtaagggtg tttttgatcg cgttcggctg ttcgtagatc tctttctgca
     1201 tgtagtgacg gtaaatacct ttatcgcccg cgtcatattg cagattggat tcgatatcct
     1261 gacgttttac ttccgcgcca gttttatcga agatgtttac cgaacggcga gtgatttccg
     1321 caatatcgcc ctcttcaagg aagataaagc gacgggtcac cggcagcagc gccagctggt
     1381 cagaagcgat aaagttttca cccataccca gaccaatcac cagcggacta ccagaacgtg
     1441 ctgccagcag ggtatccggg tgacgggagt ccatgatcac tgtaccgtac gcaccacgca
     1501 gttgcgggat agcacgcaga acagcctcac gcagagtacc gccctgtttc agctcccagt
     1561 tcaccagatg ggcaatcact tcggtgtcgg tttcagaaac gaaggtatag ccacgcgctt
     1621 ttaactcttc gcgcagcggt tcatggtttt cgatgatgcc gttatgcacc accacaatgt
     1681 gttcagaaac atgcggatgc gcattcactt ctgaaggttc accgtgggtc gcccagcgag
     1741 tgtgagcaat accggtgccg ccatgcagag gatgttcttc cgctgcctga gccagcatct
     1801 ggactttacc gaggcgacgc aggcgggtca tatgaccttc cgcatcaaca acggccagac
     1861 cggcagagtc atatccgcgg tattccagac gacgtaaacc ttcaagaagg atttctgcta
     1921 catcacgttg cgcgatcgcg ccaacaattc cacacatagt ttttgattcc gatttatatc
     1981 gttgtcggtc aacctgtatg cccgtttttt cgggcgcccc gagccttgta gagagtgggg
     2041 ttatttttat agttactgct tgtgggaggg agattatgtt atctcctcat cccatgtgac
     2101 cggattaccc ggccagaatc actttttctt taccggacga cgccagcctt ctttctgagt
     2161 ctgcggcaca cggctgatag ctaatgcatt ttcgccgaca ttacgcgtca cagttgtacc
     2221 cgcagcaatg gtcgcgcctt tgcctactgt taccggggcc accagctgag tgtcggaacc
     2281 gacaaacaca tcgtcgccga taatggtctt aaatttattc gcaccatcgt agttgcaggt
     2341 aatggttccc gcgccgatgt taacgttatc gccaatttcc gcatcgccaa ggtaagtcag
     2401 atgaccagct ttcgagcctt tacccagacg tgcttttttc atctcaacaa agttaccgac
     2461 gtgtgcacct tccaacaact cagcaccagg acgcaaacgg gcaaacgggc caatggtaca
     2521 ggccgccgcc agattcgcgt cttccacgac ggtatatggg ctaatttcgc aatcatcgcc
     2581 aatcacgctg tttttaatca cgcaaccggt gccgattttc acgcgatggc cgagagtcac
     2641 gttgccctcg atgataacgt tagtatcaat ttcaacatcg cgcccgtgag taagcgtacc
     2701 gcgcagatca aaacgcgccg gatcgcgcag cataacgcct gctaacagca gtttttcagc
     2761 ctgttcggac tggtaaacgc gctccagacg ggagagttgc aggcggttat tcacgccttc
     2821 tacttcgctt aaacgttgcg gatgaacggc gacgatttca cgcccttcct gatacgccag
     2881 cgcaataatg tcggtgatgt agtattcgcc ctgcgcatta ttgttggtca gcttcgccag
     2941 ccagcgtttc atatctgcgc cgttagcgat cagaatgccg gtgttgatct cctgaatctg
     3001 acgctgctcg tcggtggcat ctttgtgctc aacaatgccg gtaactttgc cgttttcacg
     3061 ggtgatacgt ccataaccgg ccggatcatc cagtttcacc gtcagcagac caatgccacc
     3121 ctgcggttta gcatcacgca gacgctggag tgtttcgaca gagatcagcg gcacgtcgcc
     3181 gtagagcatt aaaatgtctt catcatcggc aaagaaaggt gcggcctgct gcatggcatg
     3241 acccgtaccc agctgctctg cctgaagcac ccagttcagg ttgtcgtctt tcagcgcctg
     3301 ttttagcaga tcgccaccgt gaccgtacac caggtgaacg tgcgctgcgc ctaattcatt
     3361 cgcagcatca atgacatgct gaaccatcgc tttcccggca agggtatgca gcactttcgg
     3421 aagatcggaa tacatgcgcg tgcctttacc tgcggcaagg atcactacgc tcatagcatt
     3481 attcaacata cgcgtcctga ctgtaatttg agaacgaatt taaaccgctt caccttgaaa
     3541 aaactacatt tttttcatcg tgaaatggac agaggataaa ttgttcaata acggattatc
     3601 cccgctgacg aaagcgccat tttcgaccat cgtcacgcct tttgtctgca gaaaattaag
     3661 gcagtacctt catttaagcg tcaggtggat gtttttgctc ttatttcgat caatgaataa
     3721 acgggaaaac agggggtttt atatcagcag gatctatgtg aacgctattc aggacgggtc
     3781 acacgcgcaa aaaaaagcca gcctgtttcc agactggctt ttgtgctttt caagccggtg
     3841 ttacatcgct tttttggtca actcgataac gcgcagctgc gcgatcgctt tggccagttc
     3901 cgcagacgcc tgagcgtaat ctacgtcgcc gtgagagctg ctaatgtgct cttcagcctt
     3961 acgtttcgct tccatggctc gcgcttcgtc gagatcctgg ccgcgaattg cggtgtcggc
     4021 cagaacggtc acgttgccag gctgcacttc aagaatgccg ccagacagat agataaactc
     4081 ttcgtgaccg tgctgtttca cgatgcgaat cataccaggc ttaatggcgg tgagcagcgg
     4141 tgcgtggcca gggtagatcc ccagttcacc ttcgctaccc gttacctgga ttttctcgac
     4201 cagaccagag aacatttgtt gctctgcgct gacgacgtcc aggtggtaag tcattgccat
     4261 atcaccctcc gattaaggcg ttaaagtttt ttggcttttt ccacagcttc ttcgatggaa
     4321 ccgaccatgt agaacgcctg ctccggcagg tgatcgtatt cgccttccat gatgccttta
     4381 aagccacgga tggtgtcttt cagggagacg tatttacccg gagaaccggt gaatacttct
     4441 gccacgaaga acggctggga caggaagcgc tggatcttac gagcacgcgc taccaccagt
     4501 ttgtcttctt cagacagttc atccataccc aggatggcga tgatgtcttt cagttcctga
     4561 taacgttgca ggatggactg aacgccacgc gcggtgtcgt agtgttcctg accaaccacc
     4621 agcgggtcca gctgacggct ggtggagtcc agcgggtcaa cggccgggta gatacccaga
     4681 gacgcgatct gacggctcag taccacggtt gcgtcaaggt gcgcaaaggt ggttgccgga
     4741 gacgggtcag tcaagtcatc cgcaggtacg tatactgcct gtacggaggt gatagaaccg
     4801 gttttggtgg aggtgatacg ttcctgcaga acgcccatct cttccgccag ggtcggctga
     4861 taacctaccg ctgaaggcat acggcccagc agtgcggata cttccgtacc ggccagggtg
     4921 taacgataga tgttgtcaac gaacagcaga acgtcacgac cttcgtcacg gaatttctca
     4981 gccatggtca gaccggtcag agctacgcgc agacggtttc ccggcggctc gttcatctgg
     5041 ccatacacca gggatacttt atcgataacg ttggagtcgg tcatttcgtg gtagaagtcg
     5101 ttcccctcac gagtacgttc acctacgccc gcaaacacag agtaaccgga gtgctcgatc
     5161 gcgatgttac gaatgagctc catcatgttt acggttttac ctacacccgc accaccgaac
     5221 agaccaactt taccgccctt agcgaacgga cacatcaggt cgataacttt gataccggtt
     5281 tccagcagtt cctgagagtt tgacagctct tcgtaggaag gtgctgcgcg gtgaatcgcc
     5341 caacgctctt cttcaccgat ctcgcctttc atgtcgaccg gttcacccag tacgttcatg
     5401 atacggccca gagtcgcttt acctaccggg acttcgatcg ggtgttcgag gtcttttaca
     5461 tccagaccgc gacgcagacc gtcggaggaa cccattgcga tggtacgcac gataccgccg
     5521 ccgagctgct gctgaacttc cagcaccaga cgctcattac cattttgcac ctcaagagca
     5581 tcgtacacgc gcggtacggc atcctgaggg aattcgacgt caactacggc gccgattacc
     5641 tggacaatct ttccagtagc catcttaaat cctctacgaa ataacctgtt taaaccgcgg
     5701 cggcccccga gacgatctcg gtgagttcct gagtaatgct ggcctgacga gctttgttgt
     5761 ataccaactg cagctcttta atcaggctgc cgccattgtc ggtcgcggct ttcatcgcca
     5821 ccatacgggc ggcctgctcg ctggccaggt tttcaaccac gccctgataa acctgagatt
     5881 cgacataacg acgcagcagg gtatccagca acgccttcgg atcgggttcg tacaggtaat
     5941 cccaggattt atgtttcaga tcatcatcat ctgatgccgg taacggcagc agctggctga
     6001 tggtcggaac ctgagacatg gtgttaataa atttgttgct gacaatgtaa agcttgtcca
     6061 gacggccttc gtcgtaggcc tgcaacatca cttttaccgg accgatcagt tcggacaggg
     6121 aagggttatc ccccatgccg gtgacctggg caacaacatt gccgcccacg gagttgaaga
     6181 acgacacgcc tttcgagccg atcattgcga ggtcgcattg aacgcctttg tcggtccagg
     6241 tcttcatttc cgccagcagt tttttgaaca ggttaatgtt caaaccaccg cacaaaccac
     6301 ggtcggtcga caccaccagg tagcccacgc gtttaacgtc gcggtcttcc aggtaagggt
     6361 gcttatattc cagattaccg tgtgcaaggt gaccaatcac tttgcgcatg gtttctgcat
     6421 aaggacggct ggccgccatg cgatcctgcg atttacgcat tttggaagcg gcgaccatct
     6481 ccatcgcttt agtgatcttt tgcgtgttct ggacgcttgc gatcttacta cgtatctctt
     6541 ttgcgccggc catgagcttc tcctcaatgc cttgcggcct gccctaaggc aagccgccag
     6601 acgttaccag gattgggttg ctttgaagga atcgaggatg cctttcagct tgccttcgat
     6661 ttcgtcgttg tagccaccgg tctggttgat ctcttgcatc aacggagcgt gatcacggtc
     6721 gacgtaagcc agcagagcgg cttcgaagct gccgattttc gacagttcaa catccgccag
     6781 gtaaccacgt tctgctgcga acagaaccag agactgctgc gcaacggaca tcggcgcata
     6841 ctgtttctgt ttcagcagtt cggtcacttt ttgaccgtgg tcaagctgct tacgtgttgc
     6901 atcgtcaagg tcggatgcaa actgagagaa cgctgccagt tcacgatact gtgccagagc
     6961 ggtacggata ccaccggaca gttttttcat gatcttggtc tgtgctgcac caccaacacg
     7021 ggatacggaa atacccgggt taaccgcagg acgaataccg gcgttgaaca ggttggtttc
     7081 caggaagatc tgaccatcgg taatggagat tacgttggtc ggaacgaacg cagaaacgtc
     7141 acccgcttga gtttcgataa tcggcagcgc ggtcagtgaa ccggttttcc ctttcacttc
     7201 acctttggtg aaagcttcaa cgtattcggc gttaacacgc gcagcacgct ccagcagacg
     7261 agagtggagg tagaatacgt cgcccgggaa tgcttcacgt cctggcggac gacggagcag
     7321 cagcgagatc tgacggtaag caacagcctg tttagacaga tcatcgtaaa tgatcagcgc
     7381 atcttcaccg cggtcacgga agtattcgcc cattgcgcaa ccggcatacg gtgccaggta
     7441 ttgcagtgca gcggattcag acgcggttgc taccacaacg atggtgttag ccagtgcgcc
     7501 gtgctcttcc agtttacgta ccacgttaga aatggtggac gctttctggc cgatagcgac
     7561 atagatacat ttgataccgg aatcgcgctg gttgatgatg gcatcgatag ccagtgcggt
     7621 tttaccggtc tgacggtcac cgatgatcaa ttcacgctga ccacgaccga ttgggatcat
     7681 ggagtcaacg gctttataac cggtctgtac cggctgatct acggactgac gttcgataac
     7741 gcccggagcg attgcttcta cagcagagaa gccgtcgtga tccagcggac ctttaccgtc
     7801 gattggtgca cccagagtgt taaccacacg gcccagcagg ccacggccaa ccggaacttc
     7861 caggatacgg ccagtacact taactttcat gccttcggca aggtcagcat acggacccat
     7921 aacaaccgca ccaacagagt cgcgctcgag gttcagtgcg atagcgtaac ggttacccgg
     7981 cagggagatc atttcaccct gcatacaatc ggccaggccg tgaatgcgga taacaccgtc
     8041 acttacagaa acaatagtac cttcgttgtg agcttcactc acaacattga actgagcaat
     8101 gcgctgcttg atcagttcgc tgatttcggt ggaattcagt tgcatgctcc agtcccctta
     8161 agactgcaag acgtctgcaa ggcgctcaag acgaccgcgt acgctgccat caatgaccat
     8221 atcacccgct cggatgataa cgcctgccat tacagactta tcgattttgc aattcagctt
     8281 aactttgcgt gacagacgtt tttccatcgc agcagaaatt ttcgcgagct gttgttcact
     8341 cagtgcggca gcggaaatga cgtctacctc agcggtagcc tcactcacgg cacgaaggtg
     8401 aataaactgc tccaaaacat ccgggagcgc gttaagacga ccattttcag ccattacccg
     8461 aataaggttc tgaccgtttt cgtccagttg ctcaccacaa actgcgataa acgactcggc
     8521 aagcgtttct ggcgcaagcg cgccagagag aagctctgcc atttgttcgt ttttggttac
     8581 ctcggcggca aacgccagca tgtcctgcca gcgttctaca ctttggtgtt cgacggcaaa
     8641 gtcaaaagct gctttggcgt aggggcgagc taccgtaata aattcagaca tcagcccctc
     8701 cctccttaca gttcagcgac aagtttatcc acgatgtcgc tgttagcagc ttcatccacg
     8761 gaacgttcga tgatcttctc ggcgccagca acagccagga tagcaacttg cttacgcagc
     8821 tcttcacggg cacgtttacg ctcggcttca atttccgcct gcgcctgggc cacgatttta
     8881 gtacgttcct gttctgcctc agctttcgct tcgtccagaa tctgcgagcg gcgtttgttc
     8941 gcctgctcga tgattacctg ggcttccgct ttcgcttttt tcagctggtc ggtcgcgctg
     9001 gcctttgcaa ggtcaaggtc cttatgtgct cgttctgcgg aagcaaggcc gtcagcaatt
     9061 tctttttgac gtttttcgat ggctgccatt aatggcggcc atacgtactt catgcagaac
     9121 agaacgaaca ggacaaacgc gatggcctgg ccgaggattg ttgcgttaag attcacagca
     9181 caatgcctct atttagttaa cgttctgata ttgctcttta aataaaagca acgcttacta
     9241 cgcgacagcg aacatcacgt acagacccag acctacagcg atcatcggga tagcatccac
     9301 cagacccata acgataaaga actgagtacg cagcagagga atcagatcag gttgacgcgc
     9361 tgcgccttcc aggaatttac ccccgaggat gccgataccg atcgcagcac cgattgccgc
     9421 cagacccatc atcacagcgg cagccatgta cagcagatcc atattcaggt tttccatgac
     9481 agtctccagt ttgtttcagt taaaacgtag tagtgttggt aaattaatgt tcttcagacg
     9541 ccatcgacag atagacgatc gtcagaacca tgaagatgaa ggcttgcagc gtaatgatca
     9601 ggatgtggaa aatggcccac ggcacattca ggatccactg tgaccaccac ggcaacagac
     9661 cagcaatcag aatgaaaatc agctcaccgg catacatgtt accgaacagt cgcaaaccga
     9721 gtgaaactgg tttggacagc aggcttaccc cttcaaggat taagttgaca ggaatgaacg
     9781 cccagtgatt gaacggctgc agcgtcaact ctttcgtgaa gccgccgatg cctttcattt
     9841 tgatgctgta gaacagaatc aggataaata cgcccagtgc catagacagc gttacgttca
     9901 cgtccgcaga cggaaccaca cgcagtgcag gcagacccag tacatgttca gcaatgtacg
     9961 gcagcaggtc gataggcagt aaatccatca ggttcatcag gaatacccag acgaagatcg
    10021 tcagggccag cggagcaatc agcttgcttt tgccatggta catgtctttc acgctaccat
    10081 taacaaagcc gatcaccagc tcaatcgcgg tctgaaactt acctggcaca ccgctggtcg
    10141 cctttttggc tacgctacgg aataaaacca ggaacaacag acccagcacc accgagaaga
    10201 acatggagtc aatattgatt gtccagaagg tggctggggg gttttgtgga tccaccagcg
    10261 agaatgtacg caggtccagc tgaaggttat tcaggtggtg tcctatgtaa tcctgcggcg
    10321 tcatattttc tgaagccatg atgcctttta ccctttgttg ttaattacag ccggtgccag
    10381 tatctgaacc accagcacca aaacccacgt aacgatcagc ggcaagaata ccgcctttaa
    10441 aaccgccaac gccaccacca gtaacaccaa catcgccaga actttgaaag cttcaccaaa
    10501 tgcgaatgtc caggccaccc ggcctttcgc tggtgtatgc gcctggtgac gccaggcaaa
    10561 tatcataaac aaaacgttag gcagaaagac tgccaggccc ccgcttattg cagagacgcc
    10621 ccagaagggg tctttgaggc tgaacagcaa tccacttgct atcaccacca gtaactgaac
    10681 gagcagaagc ttccgagcaa cgtttcgact cacgagcgac acagacatca cgtttttcac
    10741 tcctgctccc ttcgaggtat gccgcgtgtc gtataaaact ttctttaagg cttagagtca
    10801 agcatcaaaa agcggtcaaa ttatacggtg cgcccccgtg atttcaaaca ataagtagcc
    10861 aaaaggtgaa taaatgttta aatatttttc cagtgcatac aattgcgact tttctactaa
    10921 ccctgttcga tcatgaaaaa ctgtaaataa cgcgtaaaca ctggtgataa agcgtgcttc
    10981 agatcacata ttgcgcatgt tcgcgcacag catatttatt tacttggcaa atgatgcctt
    11041 tgcaagttta tgatatttca gtctaaaaac agatactgtt ttaataaatg acatttacac
    11101 aacaaaaacc acccattgac atttttaata atgttttaac agccaatgat ggttcttagc
    11161 gccgattttt agcagactga tattttcact aatgacttat tttctgctta ccaaaaaaag
    11221 ccacgttatc ttgttgatgc aaaagagtga acgtggcgtt aaatgtaacc agttatatca
    11281 gtagaaaacc tggttgttgt taacagtcta accggtcaat tttttatgat ttttttgata
    11341 aaaattaaat tttatttgct ttaatcacca ccagatgacg ttcgccatcc agggctggaa
    11401 cctgaagttt aaccactgat tcgacctgat attcttcggg caacaaagcg atttcatctt
    11461 ccggcatttg ccctttcagc gcgtagaaac ggccttgctc accaggaaga tggtggcacc
    11521 agctcaccat atcgttcaga gaggcaaaag cgcggctaat tacgccatca aatggcggct
    11581 ctgaaggaaa ctcttctacc ctgctctgta ctggctcaat attctccagt ttaagctcat
    11641 gttgcacctg acgaaggaaa cgcacgcgtt taccaaggct atccaacaga gtgaaatggg
    11701 cttcaggacg cacgatagag agtggaatgc caggcagtcc aggaccggtg ccgacatcga
    11761 taaaccgttc accttgcaga tacggtgcca ccacaatgct atcgagaata tggcgtacca
    11821 gcatctcatt aggatcgcgg accgaagtca ggttgtacgc tttgttccat ttatgcagca
    11881 tattcacgta ggcaataagc tggtttttct ggtgatcggt aagcgaaata cctgcgtctt
    11941 tcagcagtaa ggagagtttg ttgagcacgg tgattacctg ttcttgatgc gttgcctggt
    12001 aagcgggggc ttaccaggca tttttaatgc gttatgcgct acgacgcagc ataccctgtt
    12061 ttttcagcca caccagcaga atggagatgg ccgcaggcgt gacgccagaa atacgtgaag
    12121 cctggccgat agaggccggt ttgtgatcgt taagtttggc gatcacttcg ttagaaagac
    12181 cggatacctg gcggtaatcc agtgtcgcgg gtagcaaggt gttctcgtta cgcagctgct
    12241 tttcgatctc atcttgctgg cgcgcgatat aaccttcgta tttaacctga atctcaacct
    12301 gttccgccgc ctgttcgtct gtcaacgcag gggcaaacgg cgtcagcgtg gttaattttt
    12361 cataagtcat ttccggacga cgcagcagat cttcaccact ggcttcacgg gaaagcggcg
    12421 cagtcaggtg agcattcact tcggctgcag cttccgccga cggggttacc caggtcgatt
    12481 tcagacgctg acgctcacgc tcgatattct caagtttctc gttaaagcgc gcccaacgtt
    12541 cgtcatccac caggcccagt tcacgaccga tttcagtcaa acgcagatcc gcattatctt
    12601 cgcgcagcat cagacgatat tctgcgcgcg aggtaaacat acgatacggt tctttggttc
    12661 ctaaagtgca caggtcatca accagtacgc cgagataggc ctgagaacgc gccggagccc
    12721 aaccttcttt gtcatcagac agacgggcag cgttaagacc ggccagcaaa ccttgcgcag
    12781 cggcttcttc gtaaccggta gtgccgttaa tctgaccagc aaagaacagc ccctggataa
    12841 acttgctctc cagcgtcggt ttcaggtcgc gaggatcgaa gaagtcatac tcaatggcat
    12901 aacccggacg cacgatcttc gcgttttcca tcccctgcat agagcggacg atttgcatct
    12961 gcacatcgaa cggcaggctg gtggagatac cgttcggata aatttcgtta gaagtcagcc
    13021 cttccggttc aaggaagatc tgatgctgat ttctgtcggc gaagcgcatg actttgtctt
    13081 cgatcgacgg gcagtagcgt gggccgacac cttcgatcac ccctgcgtac attgggctac
    13141 gatcgaggtt actgcggatc acatcatggg ttttctcgtt ggtatgggtg atataacacg
    13201 gcacctgctg tggatgctgg gacgcattgc ccataaaaga gaataccggc attgggttat
    13261 cgccatgctg ttgcgcaagc acgctaaagt cgatggttcg agcatcaata cgcggtggtg
    13321 tcccggtttt cagacgacca acgcgcagcg gaagttcacg caaacggcga gaaagcggaa
    13381 tggacggcgg atcaccagca cggccaccgc tgtagttatc cagaccgata tgaattttac
    13441 cgtcgaggaa cgtcccaacg gtgagcacga cggctttggc acggaacttc agtcccattt
    13501 gggtaacagc accgaccaca cgatcgtttt cgacaataag atcttcaacc gcctgctgga
    13561 agatcatcag gttcggttgg ttctccagcg ccgtacgtac cgcctgacgg tagagcacac
    13621 gatccgcctg agctcgggta gcgcgaactg ccggtccctt gcttgcgttt agtatcctaa
    13681 actggatacc cgcctgatcg atcgctttcg ccatcagacc gccgagtgca tccacttctt
    13741 ttaccagatg tcccttccca ataccgccga tcgccgggtt gcagctcatc tgccccagag
    13801 tgtcgatatt gtgtgtcaaa agcagagtct gttgacccat acgcgccgca gccatcgcgg
    13861 cctcggtgcc tgcatgaccc ccgccaatga tgatgacgtc aaaaggatcc ggataaaaca
    13921 tggtgattgc ctcgcataac gcggtatgaa aatggattga agcccgggcc gtggattcta
    13981 ctcaactttg tcggcttgag aaagacctgg gatcctgggt attaaaaaga agatctattt
    14041 atttagagat ctgttctatt gttatctctt attaggatcg cactgccctg tggataacaa
    14101 ggatccggct tttaagatca acaacctgga aaggatcatt aactgtgaat gatcggtgat
    14161 cctggaccgt ataagctggg atcagaatga ggggttatac acaactcaaa aactgaacaa
    14221 cggttgttct ttggataact accggttgat ccaagcttcc tgacagagtt atccacagta
    14281 gatcgcacga tctgtatact tatttgagta aattaatcca cgatcccagc cattcttctg
    14341 ccggatcttc cggaatgtcg tgatcaagaa tgttgatctt cagtgtttcg cccgtctgtt
    14401 ttgcaccgga atttttgagt tcagcctcga gtttatcgat agccccacaa aatgtgtcat
    14461 attcacgact accaataccg attgcgccaa agcggaccgc agaaagatcg ggcttctgtt
    14521 cctgcaatgc ttcatagaaa ggagaaaggt tgtccggaat atctccggca ccgtgggtgg
    14581 agctgataac cagccagatc cccgaggcag ataaatcttc caacagcgga ccgtgcagcg
    14641 tttcggtggt aaaacccgcc tcttccagct tttcagccag gtgttctgct acatattcgg
    14701 caccgccgag ggtgctgccg ctgataagag ttatatctgc cataaaccgc cacctttatt
    14761 aagagtggcg tattgtacgc tgtgaacgcg ttgggatcta cctgtggaaa agtatgggat
    14821 taaaaaagcc gatcagggct tgatggtacg catgatcggg ttctgcagga cgatcagtgt
    14881 ctcggtggac tgaatttcat caattgtttg gatcttgttg ataagtacat gctggagagc
    14941 atcgatcgaa cggcacatca cttttataaa gatgctgtag tggccggtgg tgtagtacgc
    15001 ctcggtcact tcatcgaggc tttccagctt tgccagcgcg gaagggtagt ctttggcgct
    15061 ctttaatata atgccgataa agcagcctac gtcataaccg agctgcttcg ggctgacatc
    15121 aatacgcgcc ccggtaatga tccccgcctg cttcattttc tctactcgaa cgtgaatcgt
    15181 ccccggactg acgccaaatt gtttcgccag ttcggcgtaa gcggtgcgcg cattgcccat
    15241 taatgcttcc aggatgccac ggtccagatt gtcgatcaga taattttcca taggattttc
    15301 ttatgcggat tgatgattca ttctatttta gccttctttt ttaatgaatc aaaagtgagt
    15361 taggcttttt attgaatgat tattgcatgt gtgtcggttt ttgttgctta atcataagca
    15421 acaggacgca ggagtataaa aaatgaaaac cgcttacatt gccaaacaac gccaaattag
    15481 cttcgtgaaa tctcactttt ctcgtcaact ggaagaacgt ctggggctga tcgaagtcca
    15541 ggcgccgatt cttagccgtg tgggggatgg cacgcaggat aacttgtcgg gctgtgaaaa
    15601 agcggtacag gtaaaagtga aagctctgcc tgatgcccag ttcgaagtgg ttcattcact
    15661 ggcgaagtgg aaacgtcaga ccttagggca acacgacttc agcgcgggcg aagggctgta
    15721 cacgcacatg aaagcccttc gccccgatga agaccgtctt tctccgttgc actcggtcta
    15781 tgttgaccag tgggactggg aacgcgtaat gggcgacggt gagcgtcaat tctcgactct
    15841 gaaaagcacg gtagaggcga tttgggcggg aattaaagca accgaagctg cggttagcga
    15901 agagtttggc ctggcaccgt tcctgccgga tcagatccac ttcgtacaca gccaggagtt
    15961 actgtctcgt tatccggatc ttgatgccaa agggcgtgag cgggcgatag cgaaagatct
    16021 tggtgcggta ttccttgtcg ggattggcgg caagctgagc gatggtcatc gccacgacgt
    16081 gcgcgcaccg gattatgatg actggagcac cccgtcagag ctgggccatg cgggtctgaa
    16141 cggcgatatt ctggtgtgga accctgtact ggaagatgcg tttgagcttt cttccatggg
    16201 gatccgcgtg gatgccgaca cgttgaagca tcagttggcg ctgaccggtg atgaagatcg
    16261 tctgcagctg gaatggcatc aggcgttgct gcgcggtgaa atgccgcaga ccatcggcgg
    16321 cggtatcggc cagtctcgtt tgaccatgct gctgctgcaa ctgccgcata tcggccaggt
    16381 ccagtgtgga gtatggccag ctgctgttcg cgagagcgtc ccttctctgc tgtaataatt
    16441 tatcgccgcc agcgtctgag caggcggctt cgcatcccgg tatcaaagcg ccagatatga
    16501 tcgaaaatgc gcatgatgcc gggtttgccg tgtgccgaca tcgccacggc atgaaagcga
    16561 tgctgatgta cccgctgcag ctctttcact ttactcgtca cgtcgtcagg caaccgctgg
    16621 gcgataaaat cagaaatcac caccgcatcg gcatcaaacc attccctgct ttgcaagcgt
    16681 tccataatgg cgcgaaaaca actggcaaga tcggtgccgc cacgaaactg ctggcttaaa
    16741 aaacggattg cttgttcgat gccttgtggg cctgaaagct cataacggac gatctcggtg
    16801 gaaaatagca taatataaca gcgccggttt tcagcgagag caatgcgcat caaagccagg
    16861 cagaacgctt tcgcacactg ttcattaaag ccgcccattg agccggaagt atccacgcag
    16921 acaataaacg gtccgcgcgg ctgttcgtcg taatctttat gcaccaccgg gcgttcgatc
    16981 actttttcac gccacgactc accgtgcagg cgataggtga gcaactgttt ttccaccagc
    17041 cgacggtaaa actcatactc cagttccgtt atccctagtg tcgccagttc tggcggcaga
    17101 agacgtaaaa tatcatcgct ttgttgcaga ccatcaacct gctcaggaac cgtcgccggt
    17161 tcgcgcacca tggtgcggaa ggtttccatc tgcgcatcgt tgcgcggtat tgatttggct
    17221 tcccgggaac gccccagttg ttctgccagg cgtttcagtt ccggctgttc gttaagaaat
    17281 tcaccgtatt tcacaatcaa ctgatagtcg ccacgtttaa gctgaccggc gctcatatcc
    17341 cacagacgac cagctgcggt attgttatct gcgagaatcg gttcaagttg tccgctcagc
    17401 gtcatgcgtt cctgaacttc actcaacagt tgttcgcgtt cttcttctaa tagctgttga
    17461 tttaacgttg ttgcttgcac gatcagactt aaacgccagc gctggagaaa aagcgtgtgt
    17521 aacgctgaag tgatcgtgct gttagcatcg accaactgtc gggcttgttc tgcccatggg
    17581 gaattcagac gatgcagtaa gtccaggatc tgtggtagct gcacaataaa ctgcggcgtg
    17641 gagaggagct ggctttgctg atagcacatc acctcttcgg tgagttccgg cgggactcgg
    17701 gcatctttca gccgactgcg cagcgcctca cgccagcggg gaacatcatc agtgattgcc
    17761 gccttcagtc gtgggaattt ttcaaagaag actgccagct gcggtgaggc cagcagcgcg
    17821 atgatcatct cttcgatcaa tccctcttcg ctgacggcca gcatcacatt aagcgtatcc
    17881 agcgttagca ttgttgtgcc tggcgaatct gtgcgccgac atcctgtagg ctggcttcaa
    17941 tgcgacctaa ccagtcgcca gggataaaca gacatctttg ctgttcgctg aataaagcgt
    18001 gttgcttgcg ccagtcgctt tcaagctctt ccaattgttg ttttatttca cctggcagac
    18061 cttcagccga tgaaccggga agtgccagcg tactgccttg taaacttaca tcgcgtacaa
    18121 caagatgttg ggcgctatca acttccagat tcagtttctg ggcaaagccg ataccgttca
    18181 gtttgccgcg aatttcacca cctttgctca gccactgttc cagcgcgcta cgctcaaagg
    18241 agatatgaac cacttccata ttatgcaatt ttaacggttt ttgcagcagc agagtcagag
    18301 tggaagcagt aacgttaaca ggaagttgat actgctgacg acggctgaaa atgccgccca
    18361 gacgaattac cgttaaggct gttttatcgc tttgttgctg ctggagttgc aggtgacgtt
    18421 gcacaatcgc gcccaggcgg gtcaacatcc cttgctgttg ccaggcatga ccggtcatca
    18481 atacatcaat ttgttgttgt atcaaattca ggctttgcgc gtcataccac aggcaatctt
    18541 tcagcaaaat gagatctacc ggggcaacag cactgcgacc gctaaaaaag gcgctggcct
    18601 gcaataatcg aatcgctttt ttccagcgac gatccgagac ataaggcgca tccggtaatt
    18661 tatccagttg ctggcgcagc ataaaaatca gctcaaatac atgatcgggc agcgtaattt
    18721 caccaatctc tttttgccag cgttcatatt cttcatctgt gacctgcaag gaggctggaa
    18781 ccggattgtc attttcatcc tgttgactgg tcagcatgga gcggaaattc gctttatcct
    18841 gcactttatc taaccacaga cgaatcagca tgcggtcata taacgcttcc agactgctgt
    18901 ctgcttccgg cagctcgttg gaggccgcca ccagcaggcg catcgggatt ttttctacgt
    18961 gtgcaccgtt gcggaactgg cgctcgttaa tggcggtgag cagggtatta agaattgccg
    19021 gacccgcttt ccagatctca tccagaaaga cgatttctgc ttccggcagg taaccgctgg
    19081 ttaaacgttc atagcgccct tcatctttta gcgcctgaat agaaagggga ccaaaaactt
    19141 cttccggcgt ggagaagcgg gtcatcagat attcaaacgc gcgggcattc tgaaaggcga
    19201 attttaagcg ccgggcgatc aaacttttgg caatacctgg cgggccaagg aggaacacac
    19261 tttcaccact taatgccgct aataaacaca agcggatggc gtggctacgt tcataaagcc
    19321 ccttttccaa cgaactgctc aggcgggaaa ttctttccgc taataaatga gggtgagcca
    19381 taatgaagtg gcgtcctttc gtcaaaagtt ctgcgtaaat tgcgagtata gacgtttctt
    19441 gctggtggct aaaatagtct caaagggggg gtatttttct ttgagccagg ttaatgtggc
    19501 cgcatttagg agtacgattt tgccgttaat cgtgcatact gtgcgctttt ttgtgggcca
    19561 agggactaag cacacatttc atatttcaac gaaagactag tctatgagca ctgataataa
    19621 gcaatcattg cccgcgatta ccctcgcggc gattggagtt gtctacggcg atattggtac
    19681 cagcccgtta tatacacttc gtgaatgttt gtccggccag tttggttttg gcgttgaacg
    19741 cgatgccgtg tttggctttt tatcgctgat cttctggctg ctaatctttg tggtttccat
    19801 taaatatctc accttcgtga tgcgggcaga taacgccggt gaagggggga tcctgacgtt
    19861 gatgtcgctt gccgggcgta atacgtcggc gcgaaccaca tcaatgctgg tgattatggg
    19921 gctaatcggc ggcagctttt tctatggtga agtcgtcata acacccgcta tttcggtgat
    19981 gtcagccatt gaaggtctgg aaatcgtcgc cccgcagctg gatacctgga tagttcccct
    20041 ctcaattatc gttctcactt tattatttat gattcaaaag catggcacgg ctatggtcgg
    20101 taagctgttt gcgccgatca tgctgacctg gtttttgatt ctggcaggac tggggttacg
    20161 tagcattatt gctaaccagg aagtgctgta tgcgctgaat ccgatgtggg cggtgcattt
    20221 cttccttgaa tacaaaacgg tttcttttat tgcattaggg gcagtggtgc tgtcgattac
    20281 gggggtcgag gcgctgtatg ctgatatggg gcactttggt aagttcccta ttcgcctggc
    20341 gtggttcacc gtcgtattac cttccttgac ccttaattac ttcggtcagg gagcgctgtt
    20401 gttaaagaat ccggaagcga ttaagaaccc gttcttcctt ttggcaccgg actgggcgct
    20461 gatcccgctg ctgatcatcg ccgcactggc gacggtaatc gcctcgcagg cggttatctc
    20521 tggcgtcttt tcattgacgc gtcaggcggt acgtctggga tatttgtcgc cgatgcgcat
    20581 tattcacacc tccgaaatgg agtcagggca aatctatatt ccgtttgtga actggatgct
    20641 ctatgtcgcg gtcgtgattg tgattgtcag ctttgagcac tccagcaacc tggcggcggc
    20701 gtacgggatt gcggtgaccg gaaccatggt gctgacgtct attctctcga ctaccgtggc
    20761 acgtcagaac tggcactgga ataagtattt tgttgcgctg atcttgattg ctttcctttg
    20821 tgtcgatatt ccattgttca ccgctaacct cgataaactg ctctccggcg gctggttgcc
    20881 attgagcctc ggtacggtga tgtttatcgt gatgaccacc tggaagagtg agcgtttccg
    20941 cttgctgcgg cggatgcatg aacatggtaa ctctctggaa gcgatgattg cttcgctgga
    21001 gaaatcaccg cctgttcgcg tgcccgggac cgcggtatat atgtcgcgtg caatcaacgt
    21061 cattcccttt gcgctgatgc ataaccttaa acataacaag gtattgcatg agcgggtgat
    21121 tctgttaact ctgcgcaccg aagacgctcc atatgtccat aacgtccgtc gggtacagat
    21181 tgaacaactg tcgcccactt tctggcgcgt ggtggcaagt tatggttggc gagaaacgcc
    21241 aaacgtggaa gaagttttcc accgctgcgg tctggaagga ttaagttgcc ggatgatgga
    21301 aacctccttc tttatgtcgc atgagtcgtt gatcctcggc aaacgcccgt ggtatttgcg
    21361 tctgcgcggc aagctgtact tgctgctgca acgtaatgcg ctgcgtgcac cagatcaatt
    21421 tgaaatcccg ccaaacaggg ttatcgaact gggtactcag gtcgaaatct aacgccagac
    21481 gcctcctttc ttcataaggg ggcgtttttg ttttcatggt taatcaccat gtaaaacgtt
    21541 tcgaggttga tcacatttcc gtaacgtcac gatggttttc ccaactcagt caggattaaa
    21601 ctgcgggtca gcgaaacgtt tcgctgatgg agaaaaaaat gaaaaaaggc accgttctta
    21661 attctgatat ttcatcggtg atctcccgtc tgggacatac cgatacgctg gtggtgtgtg
    21721 atgctggttt acccatcccc aaaagtacaa cgcgtatcga tatggcatta acccagggtg
    21781 taccttcttt tatgcaggtg ctgggcgtcg tcacaaatga aatgcaggtc gaggctgcca
    21841 ttatcgcgga agagatcaaa caccataatc cgcaactcca cgaaacgttg ctcactcacc
    21901 ttgagcagct gcaaaaacac cagggaaata ccattgaaat tcgttacacc acgcatgaac
    21961 aattcaaaca acaaaccgca gaaagtcagg cggtaattcg cagcggagaa tgttctccgt
    22021 atgcgaatat cattctctgt gctggcgtga cgttctgagg ccgtcatgga agcattactt
    22081 cagcttaaag gcatcgataa agccttcccg ggcgtaaaag ccctctcggg cgcagcgtta
    22141 aatgtctatc cgggccgcgt gatggcgctg gtgggcgaaa acggcgcggg taaatccacc
    22201 atgatgaaag tgcttactgg catctatact cgcgatgccg gtacgctttt atggctgggg
    22261 aaagaaacga catttaccgg gccgaaatct tcccaggaag ccgggattgg gattatccat
    22321 caggaactga acctgatccc gcagttaacc attgccgaaa acattttcct cggtcgtgag
    22381 tttgttaatc gctttggcaa aattgactgg aaaaccatgt atgccgaagc ggataaattg
    22441 ctggctaaac ttaacctgcg ctttaaaagc gacaagctgg tgggcgatct ttccatcggt
    22501 gaccagcaaa tggttgaaat agccaaagtg ctgagctttg agtcgaaagt catcattatg
    22561 gatgaaccga ccgatgcgct gaccgatacc gaaaccgaat ccctgttccg cgtcatccgc
    22621 gagctgaaat cgcaaggccg cggtattgtc tatatctccc accgcatgaa agaaatcttc
    22681 gagagttgcg atgacgttac cgtttttcgt gacggacaat ttattgctga gcgcgaagtg
    22741 gcatcactga ccgaagattc gctgattgag atgatggtgg gtcgcaagct ggaagatcaa
    22801 tatccgcacc tggacaaagc gccgggagat atccgcctga aagtcgataa tctctgcgga
    22861 cctggcgtta acgatgtctc ttttacttta cgcaaaggcg aaattcttgg cgtctctggt
    22921 ttgatgggcg caggtcgtac cgaactgatg aaagtgctct acggcgcact accgcgcacc
    22981 agcggttacg tcaccctgga tgggcatgaa gtcgttaccc gttcaccgca ggatggcctg
    23041 gcaaacggca ttgtgtatat ctccgaagac cgtaaacgtg acggtttagt gttgggcatg
    23101 tcagtaaaag agaacatgtc gctgacagcg ctgcgctact tcagccgcgc tggcggcagt
    23161 ttgaagcatg ccgatgaaca gcaggctgtg agtgatttta ttcgcctgtt caatgtgaaa
    23221 acgccgtcga tggagcaggc aattggtctg ctttccggtg gcaatcagca aaaagtggcg
    23281 attgcccgcg gtctgatgac acgccccaaa gtgttgatcc ttgatgaacc tacccgtggc
    23341 gtagatgtcg gcgcgaaaaa agagatctat caactgatta accagttcaa agccgatggc
    23401 ttgagcatca ttctggtgtc atcggagatg ccagaagtat taggcatgag cgatcgcatc
    23461 atcgtcatgc atgaagggca tctcagcggg gaatttactc gtgagcaggc cacccaggaa
    23521 gtgttaatgg ctgccgctgt gggcaagctt aatcgcgtga atcaggagta aaaaaatgac
    23581 aacccagact gtctctggtc gccgttattt cacgaaagcg tggctgatgg agcagaaatc
    23641 gcttatcgct ctgctggtgc tgatcgcgat tgtctcgacg ttaagcccga actttttcac
    23701 catcaataac ttattcaata ttctccagca aacctcagtg aacgccatta tggcggtcgg
    23761 gatgacgctg gtgatcctga cgtcgggcat cgacttgtcg gtaggttctc tgttggcgct
    23821 aaccggcgca gttgctgcat ctatcgtcgg cattgaagtc aatgcgctgg tggctgtcgc
    23881 tgccgctctc gcgttaggtg ccgcaattgg tgcggtaacc ggagtgattg tagcgaaagg
    23941 tcgcgtccag gcgtttatcg ctacgctggt tatgatgctt ttactgcgcg gcgtgaccat
    24001 ggtttatacc aacggtagcc cagtgaatac cggctttact gagaacgccg atctgtttgg
    24061 ctggtttggt attggtcgtc cgctgggcgt accgacgcca gtctggatca tggggattgt
    24121 cttcctcgcg gcctggtaca tgctgcatca cacgcgtctg gggcgttaca tctacgcgct
    24181 gggcggcaac gaagcggcaa cgcgtctttc tggtatcaac gtcaataaaa tcaaaatcat
    24241 cgtctattct ctttgtggtc tgctggcatc actggccggg atcattgaag tggcgcgtct
    24301 ctcctccgca caacccacgg cggggactgg ctatgagctg gatgctattg ctgcggtggt
    24361 tctgggcggt acgagtctgg cgggcggaaa aggtcgcatt gttgggacgt tgatcggcgc
    24421 attaattctt ggcttcctta ataatggatt gaatttgtta ggtgtttcct cctattacca
    24481 gatgatcgtc aaagcggtgg tgattttgct ggcggtgctg gtagacaaca aaaagcagta
    24541 ataacgacta caggacatct tgaatatgaa catgaaaaaa ctggctaccc tggtttccgc
    24601 tgttgcgcta agcgccaccg tcagtgcgaa tgcgatggca aaagacacca tcgcgctggt
    24661 ggtctccacg cttaacaacc cgttctttgt atcgctgaaa gatggcgcgc agaaagaggc
    24721 ggataaactt ggctataacc tggtggtgct ggactcccag aacaacccgg cgaaagagct
    24781 ggcgaacgtg caggacttaa ccgttcgcgg caccaaaatt ctgctgatta acccgaccga
    24841 ctccgacgca gtaggtaatg ctgtgaagat ggctaaccag gcgaacatcc cggttatcac
    24901 tcttgaccgc caggcaacga aaggtgaagt ggtgagccac attgcttctg ataacgtact
    24961 gggtggcaaa atcgccggtg attacatcgc gaagaaagcg ggtgaaggtg ccaaagttat
    25021 cgagctgcaa ggcattgctg gtacatccgc agcccgtgaa cgtggcgaag gcttccagca
    25081 ggccgttgct gctcacaagt ttaatgttct tgccagccag ccagcagatt ttgatcgcac
    25141 caaaggtttg aacgtaatgc agaacctgtt gaccgctcat ccggatgttc aggctgtatt
    25201 cgcgcagaat gatgaaatgg cgctgggcgc gctgcgcgca ctgcaaactg ccggtaaatc
    25261 ggatgtgatg gtcgtcggat ttgacggtac accggatggc gaaaaagcgg tgaatgatgg
    25321 caaactagca gcgactatcg ctcagctacc cgatcagatt ggcgcgaaag gcgttgaaac
    25381 cgcagataaa gtgctgaaag gcgagaaagt tcaggctaag tatccggttg atctgaaact
    25441 ggttgttaag cagtagtttt aatcaggttg tatgacctga tggtgacata aatacgtcat
    25501 cgacagatga acgtgtaata taaagaaaag cagggcacgc gccaccctaa cacggtggcg
    25561 cattttatgg acatcccgaa tatgcaaaac gcaggcagcc tcgttgttct tggcagcatt
    25621 aatgctgacc acattcttaa tcttcaatct tttcctactc caggcgaaac cgtaaccggt
    25681 aaccactatc aggttgcatt tggcggcaaa ggcgcgaatc aggctgtggc tgctgggcgt
    25741 agcggtgcga atatcgcgtt tattgcctgt actggcgatg acagcattgg cgagagcgtt
    25801 cgccagcagc tcgccactga taacatcgat attactccgg tcagcgtgat caaaggcgaa
    25861 tcaacaggtg tggcgctgat ttttgttaat ggcgaaggtg agaatgtcat cggtattcat
    25921 gccggcgcta atgctgccct ttccccggcg ctggtggaag cgcaacgtga gcgtattgcc
    25981 aacgcgtcag cattattaat gcagctggaa tcaccactcg aaagtgtgat ggcagcggcg
    26041 aaaatcgccc atcaaaataa gactatcgtt gcgcttaacc cggctccggc tcgcgaactt
    26101 cctgacgaac tgctggcgct ggtggacatt attacgccaa acgaaacgga agcagaaaag
    26161 ctcaccggta ttcgtgttga aaatgatgaa gatgcagcga aggcggcgca ggtactgcat
    26221 gaaaaaggta tccgtactgt actgattact ttaggaagtc gtggtgtatg ggctagcgtg
    26281 aatggtgaag gtcagcgcgt tcctggattc cgggtgcagg ctgtcgatac cattgctgcc
    26341 ggagatacct ttaacggcgc gttaatcacg gcgttgctgg aagaaaaacc attgccagag
    26401 gcgattcggt ttgcccatgc tgccgctgcg attgccgtaa cacgtaaagg cgcacaacct
    26461 tccgtaccgt ggcgtgaaga gatcgacgca tttttagaca ggcagaggtg acgcttggct
    26521 acaatgaaag atgttgcccg cctggcgggc gtttctacct caacagtttc tcacgttatc
    26581 aataaagatc gcttcgtcag tgaagcgatt accgccaaag ttgaagcggc gattaaagaa
    26641 ctcaattacg cgccatcagc tctggcgcgt agcctcaaac tcaatcaaac acataccatt
    26701 ggcatgttga tcactgccag taccaatcct ttctattcag aactggtgcg tggcgttgaa
    26761 cgcagctgct tcgaacgcgg ttatagtctc gtcctttgca ataccgaagg cgatgaacag
    26821 cggatgaatc gcaatctgga aacgctgatg caaaaacgcg ttgatggctt gctgttactg
    26881 tgcaccgaaa cgcatcaacc ttcgcgtgaa atcatgcaac gttatccgac agtgcctact
    26941 gtgatgatgg actgggctcc gttcgatggc gacagcgatc ttattcagga taactcgttg
    27001 ctgggcggag acttagcaac gcaatatctg atcgataaag gtcatacccg tatcgcctgt
    27061 attaccggcc cgctggataa aactccggcg cgcctgcggt tggaaggtta tcgggcggcg
    27121 atgaaacgtg cgggtctcaa cattcctgat ggctatgaag tcactggtga ttttgaattt
    27181 aacggcgggt ttgacgctat gcgccaactg ctatcacatc cgctgcgtcc tcaggccgtc
    27241 tttaccggaa atgacgctat ggctgttggc gtttaccagg cgttatatca ggcagagtta
    27301 caggttccgc aggatatcgc ggtgattggc tatgacgata tcgaactggc aagctttatg
    27361 acgccaccat taaccacgat ccatcaacca aaagatgaac tgggggagct ggcgattgat
    27421 gtactcatcc atcggataac ccagccgacc cttcagcaac aacgattaca acttactccg
    27481 attctgatgg aacgtggttc ggcttagatt tacgctgtct tttgatcaaa ttattaccat
    27541 cggttgtttt cagaagcatg aacattgctg ctgaagcaac agtaataatg cccattgtga
    27601 taaacgtata gtggaattgt tcgacagtcg ttgtgccttc cattccttca taaacgcgaa
    27661 ggacggccgc acttacagct acgcctaaac tgatagacag ttgctgcgtg accgccagaa
    27721 cactgttacc gctgctggcg ttgtcatcgg tcagatcggc aagtgtgatg gtattcatcg
    27781 cggtaaactg cgtcgacata gccatcccta atataaacaa cggcaagatc agcatccata
    27841 ttgccattgc cggtgattgc aaagagaact gagcgatcat tagcccaata atcaccgtga
    27901 tccccactaa tgtatggcga tagcccagac gacgtaagac ttgggtaacc atcgattttg
    27961 caataatgga acctaacgct gtcggtgcca tcatacagcc agcaataaac gcctgataac
    28021 caaatcctac ctgtaacatt aatggcataa ggaacggtac acagccggtc cccagacggg
    28081 ttgcaatatt gcctacgata ccgatcgaga aagtgcgggt tttaaataaa tctaatgaaa
    28141 ttaatgggtt tggcgtgcgt cgtgcatgga gaatatagag aagcagtaac ccgatgctgg
    28201 tgacaattac cgtcaaggca atccagctgg cgacaatctt ttccccgaat agctctattc
    28261 cgcttgagaa gagaacaagg ctgaggccaa acagcaaaaa gccagtgata tcgaatctgc
    28321 gtcgtgcggt ggtgaaattg ggcatatgtt tgcgcgcgta aagaaggccc gcaataccta
    28381 tggggatatt gattaaaaat atccagtgcc aggttgccca ggtgaccagc acgccgccaa
    28441 gaatggggcc taaaattggc cccaccagac ccggcatggc gacaaaattc aatactggaa
    28501 gaagttcatt acgaggataa gcgcgcagta aggccagccg agcaacaggc atcatcattg
    28561 cgccgcctat cccctgaata acccggaaga caaccagctg tggtagcgaa ttagaaagtg
    28621 cgcaggccag agaacccaat gtgaacagac tcacggcaag ggtaaaaatg cgacgcgtac
    28681 cgaagcgatc ggctagccat ccgcttaccg gaataagcat cgccaccgtc agcgtataac
    28741 tgatgatggc tgattgcatc gcgagaggag aacgattaag gctatgagcg attgcgggta
    28801 aggcggtatt cagaatagtg gcatcaagtg cctgcatgaa gaaggccatc gccgcgatcc
    28861 acggcaaacc cgccatactg cgcttctttt tatcgctcat tcaatgtcct gttatcgggt
    28921 tatcacttat caggtgagcg tagcagcgcc tgacaagctt taaatgccgc gtcgccatcg
    28981 ctttggataa tcgcatcgac tatcgcctga tgcagatcca gctttatcac tgtgtcgctg
    29041 gtaattgacg tgaagtaagt gtgataaacc gaatggaata gcgaggcgaa tgaggtcaaa
    29101 aacggattgg cgctcatttc atagatatgc tcatgccagg ccatatcgac ttcgatccag
    29161 cgttcacggc gaaagttctc ttttaatgcc gccatttcgg ccattaacgt attgagatgc
    29221 gctttctgtt ccgcggtgcc aaccgttgct gccagtaggc aagcttgtgg ctccagacag
    29281 atacgcataa ccagaaagtg atcgatgacc tgatgaaagt tctcttctgt catccaccag
    29341 gtaagcaatt cctgatcaag aaaattccag ttcgattgtg gcatgacccg ggtaccaatt
    29401 cgcggtcgcg gtaaaaccat cccttttgcc gttaacgttt tgaccgcttc gcgtaccgct
    29461 gtacgactca ctccaaattg ctcgcccagc tcaatctcac cgggcaaaat ggtgccgggt
    29521 tcatattcac cttttaagat ccgttgcgcc agcttctcag ccagaacata cgaaaggttt
    29581 ttctgtgcag ctaactgttg tgcgcttaaa ggcattacta atcttccttt ttctttttat
    29641 tcctccttag tatgccacca ggaagtgtga ttacggttgc aaaaacggca aattgcttgt
    29701 tttatggcac attaacgggg cttttgctga aaaaatgcgc ggtcagaaaa ttattttaaa
    29761 tttcctcttg tcagaccgga ataactccct ataatgcgcc accactgaca cggaacaacg
    29821 gcaagcacgc cgccgggtca gcggggttct cctgagaatt ccggcagaga aagcaaaaat
    29881 aaatgcttga ctctgtagcg ggaaagcgta t
//
LOCUS       DAZFDC010000052        29026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFDC010000052 DAZFDC010000000
VERSION     DAZFDC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.052"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            293..1159
                     /gene="folD"
                     /locus_tag="ACHJ48_002376"
     CDS             293..1159
                     /gene="folD"
                     /locus_tag="ACHJ48_002376"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO4707929.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1161..1373
                     /gene="ybcJ"
                     /locus_tag="ACHJ48_002377"
     CDS             1161..1373
                     /gene="ybcJ"
                     /locus_tag="ACHJ48_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO4707930.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1481..2002
                     /gene="ybcI"
                     /locus_tag="ACHJ48_002378"
     CDS             1481..2002
                     /gene="ybcI"
                     /locus_tag="ACHJ48_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO4707931.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(2038..3423)
                     /gene="cysS"
                     /locus_tag="ACHJ48_002379"
     CDS             complement(2038..3423)
                     /gene="cysS"
                     /locus_tag="ACHJ48_002379"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO4707932.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKVEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3597..4091
                     /gene="ppiB"
                     /locus_tag="ACHJ48_002380"
     CDS             3597..4091
                     /gene="ppiB"
                     /locus_tag="ACHJ48_002380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO4707933.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4094..4816
                     /gene="lpxH"
                     /locus_tag="ACHJ48_002381"
     CDS             4094..4816
                     /gene="lpxH"
                     /locus_tag="ACHJ48_002381"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO4707934.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAVRMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4934..5443
                     /gene="purE"
                     /locus_tag="ACHJ48_002382"
     CDS             4934..5443
                     /gene="purE"
                     /locus_tag="ACHJ48_002382"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO4707935.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5440..6507
                     /gene="purK"
                     /locus_tag="ACHJ48_002383"
     CDS             5440..6507
                     /gene="purK"
                     /locus_tag="ACHJ48_002383"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO4707936.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARCFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFC"
     gene            complement(6646..7539)
                     /gene="ybcF"
                     /locus_tag="ACHJ48_002384"
     CDS             complement(6646..7539)
                     /gene="ybcF"
                     /locus_tag="ACHJ48_002384"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO4707937.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVRTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGMPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7536..8351)
                     /gene="ylbF"
                     /locus_tag="ACHJ48_002385"
     CDS             complement(7536..8351)
                     /gene="ylbF"
                     /locus_tag="ACHJ48_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4707938.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8362..9621)
                     /gene="ylbE"
                     /locus_tag="ACHJ48_002386"
     CDS             complement(8362..9621)
                     /gene="ylbE"
                     /locus_tag="ACHJ48_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4707939.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGASGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9631..11298)
                     /gene="fdrA"
                     /locus_tag="ACHJ48_002387"
     CDS             complement(9631..11298)
                     /gene="fdrA"
                     /locus_tag="ACHJ48_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4707940.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIMPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11615..12664
                     /gene="allD"
                     /locus_tag="ACHJ48_002388"
     CDS             11615..12664
                     /gene="allD"
                     /locus_tag="ACHJ48_002388"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO4707941.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRQAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12686..13921
                     /gene="allC"
                     /locus_tag="ACHJ48_002389"
     CDS             12686..13921
                     /gene="allC"
                     /locus_tag="ACHJ48_002389"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO4707942.1"
                     /translation="MITHFRQTIEEALPWLSSFGADPAGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAARL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIRIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13932..14717
                     /gene="allE"
                     /locus_tag="ACHJ48_002390"
     CDS             13932..14717
                     /gene="allE"
                     /locus_tag="ACHJ48_002390"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO4707943.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFS
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14945..16090)
                     /gene="glxK"
                     /locus_tag="ACHJ48_002391"
     CDS             complement(14945..16090)
                     /gene="glxK"
                     /locus_tag="ACHJ48_002391"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO4707944.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16112..17413)
                     /gene="ybbY"
                     /locus_tag="ACHJ48_002392"
     CDS             complement(16112..17413)
                     /gene="ybbY"
                     /locus_tag="ACHJ48_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO4707945.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17470..18831)
                     /gene="allB"
                     /locus_tag="ACHJ48_002393"
                     /pseudo
     CDS             complement(17470..18831)
                     /gene="allB"
                     /locus_tag="ACHJ48_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            complement(18868..20259)
                     /gene="ybbW"
                     /locus_tag="ACHJ48_002394"
     CDS             complement(18868..20259)
                     /gene="ybbW"
                     /locus_tag="ACHJ48_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO4707946.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPLMEPLS
                     RVSWFVGVIVAFA"
     gene            complement(20428..21306)
                     /gene="glxR"
                     /locus_tag="ACHJ48_002395"
     CDS             complement(20428..21306)
                     /gene="glxR"
                     /locus_tag="ACHJ48_002395"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4707947.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     NVDTARQVTEAADIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21407..22183)
                     /gene="hyi"
                     /locus_tag="ACHJ48_002396"
     CDS             complement(21407..22183)
                     /gene="hyi"
                     /locus_tag="ACHJ48_002396"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO4707948.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKNI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22196..23977)
                     /gene="gcl"
                     /locus_tag="ACHJ48_002397"
     CDS             complement(22196..23977)
                     /gene="gcl"
                     /locus_tag="ACHJ48_002397"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO4707949.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24067..24882)
                     /gene="allR"
                     /locus_tag="ACHJ48_002398"
     CDS             complement(24067..24882)
                     /gene="allR"
                     /locus_tag="ACHJ48_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO4707950.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24960..25442)
                     /gene="allA"
                     /locus_tag="ACHJ48_002399"
     CDS             complement(24960..25442)
                     /gene="allA"
                     /locus_tag="ACHJ48_002399"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO4707951.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25672..26598
                     /gene="allS"
                     /locus_tag="ACHJ48_002400"
     CDS             25672..26598
                     /gene="allS"
                     /locus_tag="ACHJ48_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO4707952.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26667..27761
                     /gene="mnmH"
                     /locus_tag="ACHJ48_002401"
     CDS             26667..27761
                     /gene="mnmH"
                     /locus_tag="ACHJ48_002401"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO4707953.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            27877..28242
                     /gene="ylbG"
                     /locus_tag="ACHJ48_002402"
                     /pseudo
     CDS             27877..28242
                     /gene="ylbG"
                     /locus_tag="ACHJ48_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28763..29013)
                     /locus_tag="ACHJ48_002403"
                     /pseudo
     CDS             complement(28763..29013)
                     /locus_tag="ACHJ48_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7173 a         7857 c         7195 g         6801 t
ORIGIN      
        1 atatagacct gaagcggtaa attaccaggc tgaaaattct ttttatattg tcaggtattt
       61 cttaaattat cttaatcctt aaacaaggaa ataaatcagt tccagattta caacgccatc
      121 atggacgaaa aatgaagctt tcaatctcag cgacctcagc gacagtgcgc ctaaccttcg
      181 caagaggtcg cttcacgcga taaatctgaa acgaaacctg acagcgcgcc ccgcttctga
      241 caaaataggc gcatcccctt cgacctacgt aacagatgga atcctctctc tgatggcagc
      301 aaagattatt gacggtaaaa cgattgcgca gcaggtgcgc tctgaagttg ctcaaaaagt
      361 tcaggcgcgt attgcagccg gactgcgggc accaggactg gccgttgtgc tggtgggtag
      421 taaccctgca tcgcaaattt atgtcgcaag caaacgtaag gcttgtgaag aagtcgggtt
      481 cgtctcccgc tcttatgacc tcccggaaac caccagcgaa gcggagctgc tggagcttat
      541 cgatgcgctg aatgccgaca acaccatcga tggcattctg gttcaactgc cgttaccggc
      601 gggtattgat aacgtcaaag tgctggaacg tatacacccg gacaaagacg tagacggttt
      661 ccatccttat aacgtcggtc gtctgtgcca gcgcgcgccg cgtctgcgtc cctgcacccc
      721 gcgcggtatt gtcacgctgc ttgagcgtta caacattgat accttcggcc tcaacgccgt
      781 ggtgattggc gcatcgaata tcgttggccg cccgatgagc atggaactgt tgctcgcagg
      841 ttgcaccacc accgtgactc accgtttcac taaaaatctg cgtcaccacg tagaaaatgc
      901 cgatctgttg atcgttgccg tcggcaagcc gggctttatt cctggtgact ggatcaaaga
      961 aggcgcaatt gtgattgatg tcggcatcaa ccgtctggaa aatggcaaag ttgtgggcga
     1021 cgtcgtgttt gaagacgcgg ctaaacgcgc ctcatacatt acgcctgttc cgggcggcgt
     1081 tggcccgatg acggttgcca cgctgattga aaacacgcta caggcgtgcg ttgaatatca
     1141 tgatccacag ggtgagtaac atggcgacat tttctttagg taaacatccg cacgttgagc
     1201 tgtgcgactt gctgaaactg gaaggctgga gcgaaagcgg cgcgcaggcg aaaatcgcga
     1261 ttgccgaagg ccaggtgaaa gtcgacggcg cggttgaaac gcgcaaacgc tgcaaaatcg
     1321 tcgccgggca gacagtgagt tttgcaggtc acagcgtaca ggttgttgcc tgatcccttt
     1381 cccggcctgt atcaacaggc cggttctctt ttcagacttt tgccctctac gccccgttca
     1441 tccatgctga tagcatcgac attttgccga agcgaccatc atgccaaccg ttattacgca
     1501 cgccgccgtt cccctatgca ttggtttagg gttgggttcg aaagtcatcc ctccgcgcct
     1561 gttatttgcc ggaatcatcc tggcgatgct gcccgatgcc gacgtcttgt cgtttaattt
     1621 tggcgttgct tacggcaatg tttttggtca tcgcgggttt acccattcgc tggtgtttgc
     1681 gtttgttgtc ccgctgttat gtgtgctgat tggacgtcga tggttcaggg ctgggctgat
     1741 tcgctgctgg ctgtttttaa ccgtctcgtt gctatcgcac agcttgctgg attcggtaac
     1801 cactggcggt aaaggggttg gctggctgtg gccgtggtca gatgaacgct ttttcgctcc
     1861 ctggcaggtg attaaagtcg cgccgtttgc attgtctcgt tacaccacag cgtacgggca
     1921 tcaggtgatt atttctgaat tgatgtgggt gtggttgccg gggatgctac tgatgggaat
     1981 gttgtggtgg cgcagacgat aaccggatgc gaaaactcgc atccggcaat agcgcaatta
     2041 cttacgacgc caggtggtcc cttgcgggcc atcttccagc acgatcccca tctcattaag
     2101 acggtcacgc gccgcatctg ccgccgccca gtctttcgct ttacgggcat ccagacgctg
     2161 ttgaattaac gcttcaatct cagccacttc gctgtcgtct gcctgcgcgc cgctttgcag
     2221 gaacgcttcc ggttcttgct ccagcaggcc caatacggcg gaaagtttac gcaggtgaga
     2281 tgccattgca ttcgccgctg ccatatcttc tactttcaga cggtttactt cacgcgccat
     2341 atcaaacagc acggaatagg cttccggggt gttgaaatcg tcgtccatcg cttcaataaa
     2401 gcgcgcttca aacgcttcgc caccggcagg cgcaacggtt ttatctgtgc cgcgcagcgc
     2461 agtgtagaga cgctccagcg ccgcacgcgc ctgcttcagg ttctcttcgc tatagttcag
     2521 ctggctgcga tagtggcccg acatcaggaa gtaacgcacg gtttccgcgt cgtagtattt
     2581 cagcacatcg cgcacggtaa agaagttacc cagcgatttg gacatcttct cgcggtcaac
     2641 catcaccatc cccgagtgca tccagtagtt cacatactga ccatcatggg cacaggtgga
     2701 ctgcgcgatt tcgttttcgt ggtgcgggaa catcaggtct gaaccgccgc cgtggatatc
     2761 aaagtggtta cccagctgct tgcagttcat tgccgaacat tcaatgtgcc agccaggacg
     2821 acccgcgccc cacggagacg gccagctcgg ttcgccctct ttcgacatct tccacagaac
     2881 gaagtccatt gggttgcgtt tgtcgtcgac cacgtcaacg cgcgcgcctg cctgcagctg
     2941 gtcgagatcc tgacgcgaca gcacgccata agttggatcg gtcgggacgt cgaacatcac
     3001 gtcgccgttg tccgccacat aagcgtgacc tttggcgatc agttgttcag tgagttcaat
     3061 aatttctgcg atatggtgcg tcgcgcgcgg ctccatatcc gggcgcagaa tgttcaacgc
     3121 atcaaaatct ttgtgcattt cggcgatcat gcgatccacc agcgccacaa agctttcgcc
     3181 attttcattg gcgcgtttga tgattttgtc gtcgatatcg gtaatgttgc gcacatactt
     3241 cagcttatag ccgaggaaac gcagatagcg cgcaaccacg tcaaaagcaa caaaggtacg
     3301 cccgtgaccg atatgacaga gatcgtaaac ggtgattcca cacacgtaca tgccgacttc
     3361 cccggcgtga ataggcttaa attcctcttt ttggcgtgtc agagtattga agatttttag
     3421 catcgaagat tccgtttaga catgtgtggg taattgagtt gcgtataata cccatatttc
     3481 ccgcgcgaat cagcatacat tgcgtgatga tccgatcgtg cggttatgct ataacaccac
     3541 cctatatatg acccgaactg ggttgaagca ccaatcaaac ggaacaggat gcaaaaatgg
     3601 ttactttcca caccaatcac ggcgatattg tcatcaaaac ttttgacgat aaagcacctg
     3661 aaacagttaa aaacttcctg gactactgcc gcgaaggttt ttacaacaac accattttcc
     3721 accgtgttat caacggcttt atgattcagg gcggcggttt tgaaccgggc atgaaacaaa
     3781 aagccaccaa agaaccgatc aaaaacgaag ccaacaacgg cctgaaaaat acccgtggta
     3841 cgctggcaat ggcccgtact caggctccgc actctgcaac tgcacagttc ttcatcaacg
     3901 tggttgataa cgacttcctg aacttctctg gcgaaagcct gcaaggttgg ggctactgcg
     3961 tgtttgctga agtggttgaa ggcatggacg tggtagacaa aatcaaaggt gttgcaaccg
     4021 gtcgtagcgg tatgcaccag gacgtgccaa aagaagacgt tatcattgaa agcgtgaccg
     4081 ttagcgagta atcgtggcga cactctttat tgcagatctt catctctgcg tggaagaacc
     4141 ggcgatcacc gccggttttc tgcgtttttt agcgggagaa gcccgcaagg ccgacgcgct
     4201 gtatattctt ggcgatctgt ttgaagcatg gattggcgac gacgatccca acccactcca
     4261 tcgccagatg gcggcagcga tcaaagcggt gtccgattcc ggcgttccct gttatttcat
     4321 tcatggcaac cgtgattttc tgctcggcaa acgctttgcc cgtgaaagtg gcatgacgtt
     4381 attgccggaa gaaaaagtgc tcgaacttta tggtcgtcgg gtgctgatta tgcatggcga
     4441 cacgctgtgc accgatgacg cgggttatca ggcttttcgc accaaagtcc acaaaccctg
     4501 gctgcaaacg ctattcctcg ctctgccgtt gtttgtgcgc aaacgcattg ccgtgcgaat
     4561 gcgcgcgaac agtaaagaag ccaacagcag taaatcgctg gcgatcatgg acgttaacca
     4621 aaacgcggta gtcagtgcga tggaaaaaca tcaggtgcaa tggctgatcc acgggcatac
     4681 ccatcgcccg gcggtgcatg aacttatcgc caatcagcaa cctgcttttc gcgtggtact
     4741 gggtgcctgg catacggaag gttcgatggt gaaagtcacg gcggatgacg ttgagctgat
     4801 tcattttccg ttttaaaaaa cccgcaactt tgctgatttc acagccacgc aaccgttttc
     4861 cttgctctct ttccgtgcta ttctctgtgc cctctaaagc cgagagttgt gcaccacagg
     4921 agttttaaga cgcatgtctt cccgcaataa tccggcgcgt gtcgccatcg tgatggggtc
     4981 caaaagcgac tgggctacca tgcagttcgc cgccgaaatc ttcgaaatcc tgaatgtccc
     5041 gcaccacgtt gaagtggttt ctgctcaccg cacccccgat aaactattca gcttcgccga
     5101 aagtgccgaa gagaacggtt atcaggtgat tattgcgggc gcaggcggcg cagcgcacct
     5161 gccaggcatg attgccgcca aaacgctggt gccagtgctg ggcgtgccag tacagagcgc
     5221 cgcactgagc ggtgtcgata gcctctactc catcgtacaa atgccacgcg gcattccggt
     5281 gggtacgctg gcgattggta aagctggcgc ggcaaacgcg gcattactgg cagcacaaat
     5341 tcttgcgact catgataaag aactgcacca gcgtctgaat gactggcgca aagcacaaac
     5401 tgatgaagtg ctggaaaacc cggacccgcg aggtgcggca tgaaacaggt ttgcgtcctc
     5461 ggtaacgggc agttaggccg tatgctgcgt caggcaggcg aaccgttagg cattgctgtc
     5521 tggccggtcg ggctggacgc tgaaccggcg gcggtgcctt ttcaacaaag cgtgattacc
     5581 gctgagatcg aacgctggcc ggaaacagca ttaacccgcg agctggcgcg ccatccggcc
     5641 tttgtgaacc gcgatgtgtt cccgattatt gctgaccgtc tgactcagaa gcagcttttc
     5701 gataagctcc acctgccgac tgcaccgtgg cagttacttg ccgaacgcag cgagtggcct
     5761 gcggtgtttg agcgtttagg tgagctggcg attgttaagc gtcgcactgg tggctatgac
     5821 ggacgcggtc aatggcgttt acgcgccgat gaaaccgagc agttaccggc tgaatgctac
     5881 ggcgaatgta ttgtcgagca gggcataaac ttctctggtg aagtgtcgct ggttggcgcg
     5941 cgctgctttg atggcagcac cgtgttttat ccgctgacgc ataacctgca tcaggacggt
     6001 attttgcgca ccagcgtcgc ttttccgcag gccaatgcgc agcagcaagc gcaagccgaa
     6061 gagatgctgt cggcgattat gcaggagctg ggctatgtgg gcgtgatggc gatggagtgt
     6121 tttgtcaccc cgcaaggtct gctgatcaac gaactggcac cgcgtgtgca taacagcggt
     6181 cactggacac aaaacggtgc cagcatcagc cagtttgagc tacatctgcg ggcgattacc
     6241 gatctgccgt taccgcaacc agtggtgaat aatccgtcgg tgatgatcaa tctgattggt
     6301 agcgatgtga attatgactg gctgaaactg ccgctggtgc atctgcactg gtacgacaaa
     6361 gaagtccgtc cgggccgtaa agtggggcat ctgaatttga ccgacagcga cacatcgcgt
     6421 ctgtccgcga cgctggaagc cttgatcccg ctgctgccgc cggaatatgc cagcggcgtg
     6481 atgtgggcgc agagtaagtt ctgttaacct ggtgctcatt tgattgccgg atgcggcgtg
     6541 aacgccttat ccggcctaca ccgatccggt acccattgta ggcctgataa gacgcgtcaa
     6601 gcgtcgcatc aggcatggtg cgccgattgc cggatgctct catcactaca gcgaaataca
     6661 ggtccccgct tcgcccgcca gcgtctcttc aattcgcgat aacgccccaa tccacgcggg
     6721 tttaccacgg cttctgacat aaccactcac cgccgttacc ttcggcccca tcgaaccatc
     6781 ggctttggca aatggcgcta actcatccgg tgtggcatgg cgaatggcac gttgctgcgg
     6841 cattccccag ttttcatata ccgcatcagc atcggtgagg atcaccagtc catcggcgtt
     6901 aatttgctcg gcgagcaacg cagcggcgag atctttatca atcactgctt cactccctgc
     6961 tccgtcatcc gtcacaggca caccgccgcc gccactgcaa atcaccacat gcccctcttt
     7021 gagcaacaac tcgatggctt cgctgtcgag aattttacgc ggttgcggag acgccaccac
     7081 ccggcgcaaa tatttaccat cacgtttcat ctgccagccg taagccgctt ccagtgcctc
     7141 ttgttcttct ggctgataaa ccggaccaat aaatttctct ggctgcaaaa acgccggatc
     7201 atcaggcgaa acttcaatgc gcgtccgcac cgtcgtgaca ggcggcatct gcggctgtgc
     7261 gctcaaactc tgcgccagca tatagccaat catcccctga ctttctgcca ccagcacatc
     7321 cagcggatac ggttctacct ctttccacgc cagattctgc aatgccagca gccccacctg
     7381 cggcccgttg ccgtgaacaa tcgccagccg ataagaacgg gccaggcgcg ccagcgcggg
     7441 tacagcactg gcgatattgc gatattgatt ttctgccgtc agcgcctcac cgcgctggag
     7501 taaggcgttg cccccaagag ctacaaccag tgttttcatg gttttccttg taataattgt
     7561 tggccaagcc agaaccccag cagtgtatct gcccctgacg tatgccccag cgccagcagc
     7621 gaatcaatcg caacagcggt acgtttcggg caactcagag catgaacaaa gtgcaggagt
     7681 ggcgaggcga aatatccttg cgcggcataa cgtaaataac tgacgctcac cgctgtggta
     7741 acgagttgca gattgtcgga acaggcaaaa aacggacgac cggagcgcgc atctaaagca
     7801 ccataatacc aggccgccag cagcattccg ctcagcgtgt catcatgact tggcgttaat
     7861 ccggggcctt tacccagcca gtgccgccag tcggtcttaa cgccattgag cgcggcctga
     7921 aaacagtgac gaaactggcg taactcagca ggcagcggat cgcttgccgc caacgccagt
     7981 ggcccgaaaa gcccggtttc ctccgcgcgt tgcatccatg cagctgcaag tggttgagga
     8041 tgcgcaggcg gcgtaatacg cagcaaacaa taacgctgtg gctgtttaac cgtgaaacgc
     8101 ccgaggcgaa tcccttgagc cacaacctgt ggtcgttcat tgccgcataa tccaccgcat
     8161 aacgcatcga attgcgcacg gcgaagcacc catcctccgg ggccgaaacc actaccctga
     8221 cgatgcagcg ttaacagttc gccgctttcc gtcatcaggt taatcgcccg ccgccacact
     8281 cccgctaacc gccaggactg gcgataattc ggtgcgctac tactggcaag cagaggatgg
     8341 atgatcgtca ttacgcgttc ctcaaccaat ccccatgctt tcagccagtg cttccagcgc
     8401 ctgttcaaag cacgccagcg gtgcccgcac ggtgcctgcg ccaatctgcc cgatccccgc
     8461 ctctttatgg gcgataccgg tattgatgag tggcgtaatg ccggtttcta ccacgcgacg
     8521 aatgtccagt cccaggcacg cgccctgaaa atcccagctt gggatctgca actgcatatt
     8581 gcgttcaagg taaatttccg ccatctcttc agataccgcc ctggccgctt ccatgccact
     8641 cgcaccgaca aagcgcgtta cgccaggcgc tgcgatcatt gccgcacctc cgataccaaa
     8701 ggtttcggta atcgcgctat cgcccatatc cgggttcgcc tgctcctgcg agaagccggt
     8761 gaaaaacaga ccttgcggag tgtttacagg cgcagtaaac cagcgttcgc ccagcccgct
     8821 tacccgaatc ccgaacatat tgccgttgcg ggtcattgcc gtgacgatgc tgcctgcgcg
     8881 gatcatcgcg ccagcatcca tcgccgcctt gcagtaagcc atcgcgaggt tgaggaagaa
     8941 ctgatcggtc acgctgagga aatccatcac ttccgcgatg tgctgtttat catgatcgag
     9001 gcgagcaatt tgtggggcca gcgcacgcat taacagtgct gaggaagcaa tattgcgttg
     9061 atggaactca tcgcccatcg taatgccctg cgccatcatc gcagtaagat cgataccgcg
     9121 ctccatgcgc cccagcgccg cgcttaatac tggcattaac acatcgcgca tccagcgatg
     9181 gcgagtcagg acatcttcgc cgtaagcgcc aaaacgcatc actttgccga taccttcgtt
     9241 gaggttgcag tacgcccggt tgccgtcggt cacgttctcg accaccagca tcggcatact
     9301 ggcagaagta ataccgccca ttggcccgac ggcattcaca tggtgacaag gaatgaagtt
     9361 cacttccccc tgctccagta ttgccagcgc ctgcgcttca tctttcgccc aaccttcgaa
     9421 cagacatgcg cccacgcacg cccctttcat gggtccggtc atctcctgcc agcgcattgg
     9481 cggcccggcg tgaagcagtg ttttgccctc gtttagttcg ctgataagtg aagaagccgg
     9541 ttgcacatcc agccagtgtg gacgagcgcg acgaatttgt tcgataaccg cagcattggc
     9601 ttgcgccact gatgtaaaca tgagaacccc ttattgcaaa cgttctaata aacgagccag
     9661 ttttttattg ccgccggcga ctggcgacca ttggtaatgc acaaccggtt tgctggcgct
     9721 ttgtagctcc agcgcaaaac tgcgtaatcc gatgttaatc acggcgacgt tttccagtaa
     9781 tgacggtgtg tgttgctgtg cggcaggcga gagcggatga attaacgctg ccgccagcaa
     9841 ggtggcttcc ggtagcgaac tcacgaccgc aatccccgca tcttccagcg tggcgatttg
     9901 ctgcgagcgg cattgcgggt cacgttcagt gcctgtcacc gtggcaatgg catacagtgg
     9961 ttgattgtct gaacgcgcag cacaggcttt ttgccaggcg cttaccagcg aggcggcagg
    10021 atcggcggtc gcaccgaagc caatcacgac atcaagcagc agcacacgca cttgcggttt
    10081 agcgccgaga tcggtaatta actggttgcg taaggttggg tcgatcatcg gatggggacg
    10141 cccgacggtg tagaaatcat cgccgaggtc aataatctgg tggccatcgg cgtccagcat
    10201 catgccgtga tgatgggcat catcggcttc cacgccaagg tgtccggcaa gtaatcccgc
    10261 cgcttcggca gccagcgtac cgccggtata caaaccgcaa ataaatccgc tgctggcagg
    10321 cattatcgcg ttacgtcgcg ccgtaacgcg tgaaagcagg caagccaggc gtgcggcctc
    10381 atccagcgag gaggcaaacc agacattctc gtcgcgggcc accgccgggg tataacctaa
    10441 aaacagcgcc accgtcggtt tgccggttgc tttcatggca ttaacaattt tcagacgcac
    10501 cgcttcggca ggtggttttg aaacaaatgc cagcacttcg cttttctcgt ctgcactgag
    10561 catttccagc gccgtcagcg cactgatgcc gcccacttca cggctgaggt cgcggccgcc
    10621 aaggccaatc gcgtgagtaa ttccctcccc tgccagcgca atctgcgagc acagctcctg
    10681 aatcccggta ccggaagcgc caatgacgcc aatgttgcct tccggcatca cgttagcaaa
    10741 agccagcggc gtgccggcaa tcatcgacgt accgcagtcc ggccccatca ccagcaagcc
    10801 tttttcccgc gcgcgggttt taagttggat ttcatcttcc agcgtgacgt tatcggagaa
    10861 catcatcacg ttgaggttgc gatccagcgc ctgattcgcc agctccgccg catactcgcc
    10921 agccactgaa atcagcgcca gattggcatc gggtaatttc tgacaggcac tgtcccagcg
    10981 acgcacctgc gtcaacgcct ggctgctgcc tgacccctgc gccagttgtt ttagcgcctc
    11041 ttcaagctgc tgcataatcg cctgcgcgat ccccgcatcc gccgcttcgc tacgaattgc
    11101 cacgcaaata tcgttcggcg tggcgttatt aaaatcgtca tgccagaaac ctgtggtatc
    11161 taataacgct ttattggcgg gcgtacccat cattacggaa acatcatcaa cattttctga
    11221 ttcgctgagt tttcgtgaaa taatcattaa actgaccgaa tcctgaaaac accctttttt
    11281 aataaaggcg tggatcataa caactcctta aaaattgcac ccatggcaat tgcacgagca
    11341 gtgttaatgt ggcgattacg gtatcgatga caacgagtaa ataaggtgat gaatatcaca
    11401 cagggattat taacaaaaga attcaaataa ccgccatgct attacgtgtt attttttaat
    11461 aagcgtggtt gttttaaaaa ccaggttaaa agcgagcgga ttatcaaata aattctaact
    11521 atattttttt gcctgtctgg atcacataat ccagatattt tccctgtatg ttaattgcag
    11581 tcatgcttca caccgtcgtt aaaaaggaag acagatgaaa atcagtcggg aaacactcca
    11641 ccagctaatt gagaataaac tctgccaggc tgggttaaaa cgtgagcacg ctgcaaccgt
    11701 ggctgaagta ttggtttatg ccgatgccag agggatccac tctcatggcg cggtgcgcgt
    11761 ggaatactac gcggaacgca tttcaaaagg cggcaccaac cgcgaaccgg agtttcgtct
    11821 tgaggaaacc gggccgtgct cggcaatttt acatgccgac aatgccgccg gacaggtcgc
    11881 ggcgaaaatg ggtatggaac atgccatcaa aaccgcccag caaaatggcg ttgcggtggt
    11941 cggtatcagc cggatgggtc acagcggcgc aatctcttat tttgtgcagc aggcagcccg
    12001 cgccggatta attggcattt cgatgtgcca gtccgatccg atggtggtgc cgtttggcgg
    12061 cgcggaaatt tactacggta ctaacccact ggcctttgcc gcgccgggag aaggcgacga
    12121 gatccttacc tttgatatgg cgactaccgt acaggcatgg ggaaaagtcc tcgacgcccg
    12181 ctcgcgtaat atgtctatcc cggatacctg ggcggtcgat aaaaacggtg caccaacaac
    12241 cgatccgttc gcggtacatg ctctgctccc cgcagccggg ccgaaagggt atggcctgat
    12301 gatgatgatt gacgtcctct caggcgtctt actcggctta ccgttcgggc gacaggttag
    12361 ttcgatgtat gacgatttac acgccgggcg taatttgggg caattacatg tagttattaa
    12421 cccgaacttt ttctcctcca gcgaattatt ccgtcaacat cttagccaga ccatgcgcga
    12481 attaaatgcc attacccccg cgcccggttt taatcaggtt tattatcccg gacaggatca
    12541 ggatattaaa caacgccaag ccgccgtcga aggcatcgaa attgttgatg atatttacca
    12601 gtatttaatt tccgacgcgc tttataacac gtcatacgaa acgaaaaatc cctttgcgca
    12661 ataattattg agacaggact ttcttatgat tacacatttt cgtcaaacca tagaagaagc
    12721 gctgccctgg ctttcctctt ttggcgctga cccagcgggt gggatgaccc gtttacttta
    12781 ttcgccggaa tggctggaaa cccagcagca attaaaaaaa agaatggccg caagtgggct
    12841 ggaaacacgt ttcgatgaag tggggaattt atacggtcgc ctgagtggca ccgaatatcc
    12901 acaggaagtg attctgagcg gttcgcatat cgataccgtg gttaacggcg gtaacctcga
    12961 cgggcaattc ggcgcgctgg cggcgcggct ggcaattgac tggctgaaaa ctcaatacgg
    13021 cgcaccgctg cgtacggttg aagtggtggc gatggcggaa gaagaaggta gccgcttccc
    13081 gtatgttttc tggggcagta aaaatatctt tgggctggcg aatcctgacg acgtgcggaa
    13141 tatctgtgat gccaaaggaa atagttttgt cgatgcgatg aaggcttgcg gatttactct
    13201 gccggacgcc ccgctaactc cgcgtcagga tattaaagcc tttgtcgaac tgcatatcga
    13261 acagggctgt gtgctggaaa gtaatgggca atcaattggc gtggtgaatg caattgtcgg
    13321 gcaacgtcgc tatacggtga cgctgaacgg cgaatcaaac catgcaggca ccacgccgat
    13381 gggttatcgt cgtgatacgg tttacgcttt cagtcgcatt tgccatcagt cgatcgaaaa
    13441 agcgaaaaag atgggcgatc cgctggttct gacctttggg aaagtagagc cgcgcccgaa
    13501 tacggtgaat gtggtgccgg gtaaaaccac gttcaccatt gattgtcgtc ataccgacgc
    13561 cgccgtgctg cgtgatttca cccaacagtt agaaaacgac atgcgggcga tttgcgatga
    13621 aatggacatt cgtattgata tcgatttatg gatggacgaa gaacccgtgc cgatgaataa
    13681 ggacctggtc gccaccctga cagaattgtg tgaaagtgaa aaactgaatt accgggtgat
    13741 gcacagtggt gccgggcacg acgcgcaaat tttcgcgcct cgcgtaccaa cctgcatgat
    13801 ttttatcccc agcatcaacg ggatcagcca taacccggcg gaacgcacca atattaccga
    13861 ccttgccgaa ggggtcaaaa cgttggcact catgctttat caacttgcct ggcagaaata
    13921 aggagtcata aatgggatat ttaaataacg tcaccggtta ccgcgatgat ttactggcta
    13981 accgtgcgat tgtgaaacac ggtaatttcg cactgttaac accagacggt ctggtaaaaa
    14041 atattattcc gggctttgaa aattgtgacg cgacaatcct ctccacgcca aagctgggtg
    14101 cctcttttgt tgattatctg gtcacactgc atcaaaacgg cggcaaccaa cagggcttcg
    14161 gtggcgaagg cattgaaacg ttcctgtatg tgatctctgg aaatatcact gccaaagccg
    14221 aaggcaaaac attttcctta agcgaaggtg gctatcttta ttgcccgcct ggctccttaa
    14281 tgacgtttgt taacgcccag gccgaagaca gccaaatctt tttatataag cgccgctatg
    14341 ttccggtaga aggctatgca ccgtggctgg tttctggcaa tgccagcgaa ctggaacgca
    14401 ttcactatga aggtatggat gatgtgatcc tgctggattt cctgcccaaa gagttaggtt
    14461 ttgatatgaa catgcatatc ctttcttttg caccaggtgc cagccacggt tatatcgaaa
    14521 cacatgttca ggaacacggt gcctatattc tttccggtca gggggtttat aacctcgaca
    14581 ataactggat cccggtgaaa aaaggcgatt acatctttat gggcgcttat tctttacagg
    14641 ctggttatgg cgtagggcgt ggtgaagcgt tcagctatat ttactcgaaa gattgtaacc
    14701 gcgacgtaga gatttaagtc ataccactcg cctgattatt tttagctatg ttggattttg
    14761 ccacggttta taccgtggct ttttttgcaa catagcctga tgcgcttcgc ttatcaggcc
    14821 tacatgatct ctgcaatata tagaatttgc gcgattttca ggaaggatat ggtactcatg
    14881 ccgcatccgg catgagtact gcactcttat gcgatataaa tcgcatccgc tttaaaggta
    14941 agggtcagtt tttaattccc tgacctattt taatggcgca ggcaatattt cgcgcgctgt
    15001 tgaagagatt ggtttcaccg ctggcgagca cttcggctaa aggtgccaga cgtggcaaaa
    15061 tgctgaatac cgcatcaatg ccgtactggt gcaccacttc cacgccatca cccaatacgc
    15121 cagcaatccc aatcaccggt acattaaact gcttcgccac cgacgcaacg cccagcggcg
    15181 ctttaccgcc tgccgtttgc gagtcaatgc gcccttcccc ggtgatcacc agtgctgccc
    15241 cctgcaccgc ctgctccagg ttgaccgccc gcagtacaat ctcaataccc ggtttaatat
    15301 ccgcattgag gaataccgcc gccgcaatcc ccataccgcc cgcagcgcca ccgcccgcca
    15361 tctggcagac attaatttca gtttgctgtt gtaaaacacg ggcgtaattt tgcagcccct
    15421 gttcaagttc ttcgaccatc tccggcgttg cccctttttg cgggccaaac accgccgccg
    15481 cgccgcgtgc ccctaccagc gggttatcaa cgtcacatgc tacttcaata tggcagttcg
    15541 ccagacgcgg atcgcattcc tccatctcaa tgcttgccac gcgcgctaaa ttgccgccgt
    15601 tagccaccag cacctgaccg tcggcatcaa ggaaacgcac accgagcgcc tgcgccatgc
    15661 ccataccgcc gtcgaccgtc gcactgccgc caatgccgag aataatatgg cgaatgccgt
    15721 tatccagcgc atgacgaatt aactcccccg taccaaaact ggaggccagc aacggattac
    15781 gcttttcagg cgcgaccagc atcaggccac ttgctgccgc catctcaatc accgccgttt
    15841 tcccgtcgcc ggtaatgcca taaaaagcat tcactttttc gcccatcggc ccgcagactt
    15901 caagcgtcac gatgttgccg cccgtcgcgg cgaccatcgc atccaccgtc ccttcgccgc
    15961 catccgctat cggcaaacag atatagttcg catcgggaaa gagggtcgaa aacccggctt
    16021 taattgcctg acaacatttt tctgcactca agctctcttt aaaagagtct ggcgcaatga
    16081 caatcttcat tatcaatcct ttttcaacaa attattcgat gcgttcccac ggaataaggt
    16141 tatccataag aacagccagt aaaatcccga ccaataagcc gttactgagc agaggacgaa
    16201 gcgttaatgg aaggtcttgc agatacacag gcggtaatgc cattaaaaat atgccgacaa
    16261 ataacggcaa tgcgagtcga taaatattgc gagcggtaaa cgttatttgc tggctaaaca
    16321 ccagcgcgga aaagagtaaa ggtagataag aaaccagcat gaccgcacta ctcacgggta
    16381 aagggatact gcaaaacagt cgcgtgagtg ccggaaccag cgccaccagc aggcaaataa
    16441 cgctgccata aataaacgaa cgccgcgtgt aatcgccagt ctgggttaat aaaccaatgg
    16501 atgaaacaaa cggtgaaaat ggaattactg ccagcggcac ggttatcagt gtcataaatc
    16561 cggtcgccac aaagctacga cgataacgcg tattccccgc gccctgctgc ggataaaaaa
    16621 catccgtgcc ccgaatcgca ccgtaggtat tgctgatatt caccagacct gtaatcaccg
    16681 ccgtcagaat aatccccggc gacaaagcac cgccactgcc gagcgggaac cactgccagt
    16741 gcaactcacc ggagagcgaa tgcgaagaag gaaagcaaaa gtaccacaac aaccagccgg
    16801 ttatggtgcc gaccagcagg ccataacggg caaaacgttg cggcagaaag ataatcatcg
    16861 ccagtaccag gcacatcacc gccaccgaga gcgcgaacgg cggtaactga attttaaaat
    16921 tcgggtcggc tatgccaaac ggcagcccga gcataccttt gaaaaagata gtggtcagct
    16981 gcgcgcccag catcaacata aacaagacca tcaccgacgg cgtaaataac cgcgccaggc
    17041 gatgacctaa tccgctaaaa ccaatcaaca tcgtcagcac gccggagagt gcaatcccca
    17101 ccgccaggct ggtagcgata tcgttgatcg gtgtcccgcg cgatgcttcg ccaagggtga
    17161 tagttaggat ggttccccac cacaggcccc ccggcccttc cataatagcg cgacgatgac
    17221 cgcaaaacgc ctgagcgaag caggccagtg cggtagcaag aaaagcatat tgcgtgagcg
    17281 taagcaggct actttgcggc aactgaaaag cagaaagtag cgtaggagga accacaaccg
    17341 tgttgcaaaa aataaagaaa aaccactgaa atcctgataa caggctttcg cggctaactg
    17401 caaaattgaa cataaacaca ccttaaccgg agaatgcccg ccgcaaggac gggcattgca
    17461 ggggccagat tactgctgat gtttaaggat aaattgacct ttcggcgcaa cagggaagcc
    17521 ctgttcgatg tcgtaaatca catcaccacg taagatggtt ttcgtgatac gcgcgccaat
    17581 ggtacggcca acatacgggc tgactttgtg gcgatattcc agatcgtcat tggtaagaac
    17641 atagctgcta ttcggctgaa tgaagacgaa gtcggcatct tttcctgggg cgatacggcc
    17701 tttttgctgc agaccaaaaa tatctgctgc gttagtcgcc attaatttgc cgaacattgg
    17761 cagagacatt ccgcgtttct gtaccgcttc atcgaacatc acgtccatac agttttgcag
    17821 accggcaata ccgccccatg cttccatgat gttgccggct ttcatttccg gagggcatgg
    17881 tgagtggtcg gaaaccaggc ggtcgatttc accgttaaac agtttttccc acatgccttt
    17941 ctagttttcc agatcgcgga tcggcggtga acacttcgcc agagtaccga tttcttcgaa
    18001 ctgatcggta tccagtacga agtaatgcgg gcaggattca caagtaacgt cctgaccttc
    18061 ctgacgtgca cgagtcactt cttcaacgcc ttccgggctg ctgatatggc aaacgtgcag
    18121 acggcaaccg gcaactttcg ccagatacag cacgcggcga atcgcttcca cttcggtaaa
    18181 taccggacgc gaagccacat agtcatgggc agttacgcga ccttcacgtt tcgcttcttc
    18241 gcccagtgcg tcacagatca gcgcgttttc gcagtgcacc agcaccggct gccccagttc
    18301 gcccagcttc tgcgcacctt tgaagaactg ccagtcgttt acatcacgga agtcgttgtc
    18361 gataccgcga tcgccacagg tcgcaacgaa gcatttgaag ccgacaacgc ccacttcatc
    18421 cagctcatgc agacggtcga tgttgtaaga caccaggcca ccgagttgtg ccgcatcgat
    18481 agtcagcttg cctttagcgg catcgaactt cagttcaatt gaagcgcggt caaccgttgc
    18541 aggcagctgg ttgagcggca tttcgatcat ggtggtgata ccaccttttg ccgctgcgcg
    18601 agtaccggtt tcataacctt cccagtggct acgacccggt tcagaaatat gggtgtgcgc
    18661 atcaaccatg cccggcgaaa ccaccagacc agacgcatcc ataacttctt ttgcatcgcc
    18721 cagatcctga ccgatagcag caattttgcc gcctttaacg gcgatatcta caacgcgagc
    18781 ttcgttttct aaaataacgg tgccgttttt aatgattaaa tcaaaagaca taacaaactc
    18841 cttgtaaaat acagattggt ttttaactca agcaaaggcg acgatgacgc cgacaaacca
    18901 tgaaacacgc gataacggtt ccattaaggg aataaactta ccgccaagag ataaaataac
    18961 ggccaccaga gttactgaaa acgcggtgag gttaaaaccg ttatcgtaat atttataatc
    19021 gccaggtgcg gtgtacagtt catcaagatt aatttgccca cgcatcacca caaaataatg
    19081 cgccatcatg acaccaatta ccggaccaag cattccgccg ataatatcga ggaacagata
    19141 aatactgtcc tgattttcca ttaatttcca cgggcagatc agcaggctga taatactggc
    19201 aatcagtacg ccgtttttat aggtcagttt tgtcggtgca atggcagcaa tctgataacc
    19261 agccggaata atattaccag tggcgttagt ggagatagtc gtcatcagaa taaccagtac
    19321 cgcaaagaac gaggcaaaca ggctgtccca acgctgaaca atatccagca cgttccaggt
    19381 atcagcgccg taatgaatac tggctccggc aataatacat acccccgcga ccgcaaacag
    19441 aatataggcc acaactaaac ccagcgtttg ccccagcgct tgctcacgaa acgagtgggc
    19501 gttttgcgta aagtcggatg cgctcaccgc cggtgccgcc cagaccgcaa ctaccgcgtt
    19561 aatcaccacc aggaacaaga atccactgtt ttctgctttc tgaataccgc tcggaatgta
    19621 gtcaaagatt ggaccgagcc cgaccagcga aatcgcccaa atcgccatac cgccgaaaac
    19681 gatatagatg cacgggttaa gaatggcagt gaatttattt aaaactttgc caccgccaaa
    19741 acctatacca acgttgacca gccagaagag taagaaagta attaagcccg gtagagaaag
    19801 gcctaacaga gtgaaatcac caccgagagt caaaaatccc ggccagattt tgccaatcag
    19861 aatcaagcag gccagtgacc ccgcgtaaca ttgcaggcca aaccacataa tggcagcaat
    19921 accgcctctt aataatccgg gaaacagtgc gccacgtacg ccgtaagaag cacgcaggat
    19981 catggcaaac ggtacgccgt atttactgcc cgcagcgccg tttaatacca ttaccgcggc
    20041 aatgaaaaag gcgctgagga taattgccag cataatacta aaggtagaca agccgagaat
    20101 aaaaaagccg ccgaccatca cataattggg aacgttatga accgaaccca tccataaggt
    20161 aaaatagtta aatgttttcc aggtccgctg gctttgcgtt ttcggcaata gatcttcgct
    20221 atagccgcgt tgctggaata gttttctctg atgttccata aatccctctt aatttcacgt
    20281 atcgaataac gttgttttaa tctagctatg aggaaaaaat aaaataaaaa tattattttt
    20341 aatactgaag caacatcttt ggctgctttc ttttttaata ttgctggccg attcattttt
    20401 tgatatcggc catttttatt gcgggtatca ggccagttta tggttagcca ttaattccag
    20461 cgcctgcact aacgcagagt gatccaactg gctgccaccg tttgccgcgc aggtattaaa
    20521 taactcctgg caggtcgcag tgtttggcag gttcagagcc agtgccttcg cactttgcag
    20581 tgccaggttg agatctttct ggtgcagcgc aattttgaaa cctggattaa aggtgcgttt
    20641 aatcatgcgc tcaccgtgaa cttccagaat acgtgaggaa gcaaagccgc ccatcagcgc
    20701 ctggcgcaca cgtaccgggt ctgcaccggc ttttgaagca aacagcagag cttcagagac
    20761 cgcttcaata ttgagcgcta cgataatctg atttgccact ttgcaggttt gaccgtcgcc
    20821 gttaccgccc acaagggtga tatttttacc gagcagttca aacagcggtt taacacgttc
    20881 aaataccgct tcgtcaccgc caaccataat tgacaacgtc ccttcacgcg cgccgatttc
    20941 gccgccggag actggcgcat cgagataatc accacccagt tcattcacct gacgagcgaa
    21001 acgtttagtt tcaatcggtg aaatggagct catatcaaca atggttttgc ccttcagcga
    21061 ggctttggta caaccatttt caccaaacag aacttcttca acctgaggtg tgtccggcac
    21121 cataataaaa atgatgtccg ctgcttccgt tacctggcgc gcggtgtcga cattaacagc
    21181 accgagtgac agtaattcat cagcaaccgg tccaatggtc gtgacatgta attgatgacc
    21241 ggcacgcgcc agattaatgg ccatcggagt acccataatg cctaagccaa taaatcccag
    21301 tttcataatt aacctctttt taaatgtcgc tattcctgaa ttcagacaac acgatcccct
    21361 gccaacttag cagcctaaaa aagcattgtc tgaatagcgt tacgttttaa cggtacggat
    21421 ccatccagcg taaaccggct tccgtggtgg tttggggttt atattcacac ccaacccaac
    21481 cgttgtagtc agaattttcg attaccttaa agagataatc ataattaatt tctccggtcc
    21541 ccggttcgcc gcgatgcgga ttatcggcaa tttgcaggtg accaatttta tcagcccatt
    21601 gcgtcatagt gttggttaat tcaccttcca tccgctgcat gtgataaatg tcatactgaa
    21661 ttttcaaatt gcagcaacca acatcatcaa tcaatttcag cgcctgccga gttccggtga
    21721 gatggaaacc aggaatatca aaatggttaa taggttcaat cagtaataaa atatcttctt
    21781 tcatcagcat attcgcggca taacgcaggt tttctacaag cgttgcgtga atctgttcac
    21841 tgctgaaacc agccggcgtt ttaccgacca gacagttaat atttttatta cccagcgcac
    21901 gggcataacg aatcgctgct gctacgccat cccgaaactc ttcttcacgg ccaggaatac
    21961 aggcaatacc gcgctccccc gccgcccagt cacccgccgg taaattgtgc agcgtatgtt
    22021 cgagtttatt actcgccagc acctgtttta attcttcaat gtcgtagtca taaggaaaca
    22081 taaattcaac gccgcgaaaa ccacactgcg cagctttctc aaaacgggcg agaaaatcat
    22141 attcaccaaa taacatcgat aaattagcag agaaacgtaa cattatttat ctcccttatt
    22201 catagtgcat gaagcaggtt tcagtcggtg catccgctgc gttatcggcg atatcttcaa
    22261 attccatgac gttatccagt tcgctgccca tcgaaatatt tgtcacacgc tcgagaataa
    22321 cttccacgac taccggtacc cgatattgcg ccattaaggc tttcgcctgt tcaaaggccg
    22381 gtgcaatatc ttccggtttg aagacgcgaa tagctttaca acctaaacct tccgctactt
    22441 ttacgtggtc gacgccgtaa ccgttcactt cgctggagtt gatattctcg aaagcgagtt
    22501 gcacgcagta gtccatgtca aaggcgcgtt gtgactgacg aatcaggccg agataggcgt
    22561 tattcaccag cacatggatg tacggaatgt tgaactgcgc gccaacggct aactcttcaa
    22621 tcaggaactg gaagtcgaag tcaccggaaa tcgccaccac attgcgtttc ggatcagcgg
    22681 cacaaacccc cagcgccgcc ggaatcgtcc agcctaacgg accagcctga ccacagttga
    22741 tccagtggcg gtctttaaag acatgcagca tttgtgccgc agcgatttgt gacaggccaa
    22801 tggtggtgac ataacaaaca tcgcgaccaa aggctttgtt catctcttca tacacgcgct
    22861 gcggtttcac cggcacgttg tcgaaatggg ttttgcgcag caaagtgcgt ttgcgctgct
    22921 ggcaatcggc gacccattct ttacgacacg gcagacgacc cgctttttgc atctcctgcg
    22981 ccacttcaac cagcagtgtc agcgccgctt tagcatcaga gacaatgccg agatccggac
    23041 acagcacgcg gccaatttgc gtcggctcaa tatcgatatg aacgattttg cgcccttcgg
    23101 tgtatttctc taccgaaccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca
    23161 tgtcggacgc cagcaacgtt gcgttaccgt aacgatgcgc ggtttgcaga ccgaccatcc
    23221 cggccatcag ttcatgatcg tccgggatac agccccagcc catcagcgtt gggatcaccg
    23281 gaacgctggt cagttcagca aactgttgta acagtgcagc tgcgtcagca ttgattaccc
    23341 cgcccccggc aacaatcacc ggacgttcgg cctggattaa catttctaca gctttttcga
    23401 tctgcatacg gctggcagca ggtttgtaga ccggcagcgg ttcgtacatg tcaggatcaa
    23461 actcgatttc cgcaacctga acgtcgaacg gtaaatccac cagtaccgga cccggacgac
    23521 cagaacgcat caggtgaaat gcctgttgca gcacgcgagg caccagcgcc gcttcacgaa
    23581 ctgtaaccgc cattttgctg accggtttag caattgcttc aatatctacg gcctgaaaat
    23641 cttctttatg cagacgggcg cgcggtgcct ggccggtaat gcacagaata ggaatggaat
    23701 cagcagaagc ggaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca
    23761 gacatacgcc gatattccct gccgttgcgc gggtataacc ttccgccatg tgcgaagcac
    23821 cttccacatg acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga
    23881 acggattgat tgcagctccc ggaacgccga aggcggtggt gataccttct ttctccagca
    23941 catacattgc cgcgtcaacg gctctcattt ttgccatttt attcctacct ccattaattg
    24001 gaaaaatttt ccaactttca aattttgtca aaaaaacccg ctccgcagag cgggatgcga
    24061 cagacactat ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct
    24121 gaccagctca ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat
    24181 ggagatagcg gcaacaacgc tacctacatc atcgtaaatt gctgaagcta tgcaattcag
    24241 acctacaaca tgctcttctt tatctacggt atagcccagt tcacgcgctt gttccaggtc
    24301 cttcagcagg gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg
    24361 cagaatgatg ctcatcaact cctcttccgc cagcggataa agcagcgctt tgcccgcacc
    24421 ggaagcatgc agtggcagac gactgcccag tggcgcgcac atcctgacca tcgatttaca
    24481 ctctaactga ccaattaata ccgcttcatt gccgttacgg atcgcgacat tgaccgtttc
    24541 gccggaaagt aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt
    24601 atggatgtac gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaactg
    24661 actgtcctga tagacaaaat ccgctgcctg taaaaccttc agcaagcgaa aggtcgtgga
    24721 gagcggcaaa tccagattga gagaaatatc gctaaccgac gaacttcccc cacttttttc
    24781 cagatattgc agaatcgcaa ttccccgctc taatgcctgt gcgcccttct gtgccacagg
    24841 ctccgcctgt cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac
    24901 ctacaagtga gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt
    24961 cacgcaaaac agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg
    25021 cgatcgatgg tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg
    25081 ttacgatggt aattcactcc ctgttcgccg ttggtaataa acgcccgcag ggttgacagg
    25141 tctggtttgt cgtcacctaa cgctacgacc accacaaaca cctcaccttt catcgggata
    25201 aaggcctgag tacccagcgg atgacgttcg agttcgtgaa tggtcagcgg cagattcgcc
    25261 ggttgcgctc ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc
    25321 aaatcgtggt aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgcgtt
    25381 tcgattacgt cgccataagc actaaaggct tcctgactta acggtaatac ctgaagtttc
    25441 atctttttgt ccttgtaaca taattccgtt caaccagcgc cgaccggtca gcttggtttc
    25501 aataacaggc aatctaaaac cttaagagaa caacatcaac acaaaacttg gaatattttt
    25561 ccaaaactag atttagtcca caaattgaac agggttatca tgtaaaaata ctgataaatc
    25621 agaggataag aaaatcacgc aacttgcgga tttaaaaggg gatacctgaa gatgttcgat
    25681 ccagaaacct tgcggacttt cattgcggtt gctgaaacag gaagtttttc aaaagcggca
    25741 gaacgattat gtaaaaccac ggcgacgatc agttatcgca ttaaacttct ggaagagaat
    25801 accggagtgg cgctgttttt tcgcacgact cgtagcgtaa cgttgacagc ggctggcgag
    25861 catctacttt cccaggccag agactggctg agctggctgg aaagtatgcc cagcgagctg
    25921 caacaggtga atgatggcgt ggaacgccag gtgaatattg tcatcaacaa cctgctctac
    25981 aacccccagg ccgtcgccca gttgctggcg tggctgaatg aacgttaccc ctttacccag
    26041 tttcacatct cccgacaaat ctatatgggc gtctgggact cgctattgta cgaaggtttt
    26101 tcactggcta tcggcgtcac gggaaccgaa gcgctggcaa atacctttag tcttgatccc
    26161 ttaggatcgg tgcaatggcg atttgtcatg gcggcggatc atccgctggc gaacgttgaa
    26221 gagccgctaa cagaagcgca gttgcggcgc tttccggcgg tcaatattga agacagcgcc
    26281 cgcaccttaa ccaaacgcgt cgcctggcga ttgccagggc aaaaagagat tattgttcct
    26341 gatatggaaa cgaaaatcgc cgcccatctg gcgggcgttg gcattggttt tttgccaaaa
    26401 tcgctttgcc agtcaatgat cgataatcaa caactggtca gccgggtaat cccaacgatg
    26461 cgccctcctt cgccattgag tctggcatgg cgcaaatttg gcagcggcaa agcggtagaa
    26521 gatattgtga ccttgtttac ccagcgcagg ccggaaatca gcggattttt agaaattttc
    26581 ggcaacccac gcagttaaaa atcaagtttc cttgcgcgtt ggcgcgtaaa atagcgcgca
    26641 agctgatttg aggttcactt ttaactatgc aagagagaca cacggaacag gactatcgtg
    26701 ccctgctgat tgctgatacg cccattattg atgttcgcgc ccctatcgag tttgagcaag
    26761 gcgcaatgcc cgccgctatc aatctgccgt taatgaataa cgatgaacgc gccgccgttg
    26821 gcacctgcta taaacagcaa ggctcagacg cagcgctggc gctgggacat aaactggttg
    26881 cgggtgaaat tcgtcagcag cgcatggacg cctggcgggc agcctgcctg caaaatccgc
    26941 aaggtattct ctgctgcgcc cgtggcggtc agcgctcaca tattgtgcaa agctggttgc
    27001 atgcagcggg gattgattat ccgctggtgg aaggcggtta taaggcactg cgccagaccg
    27061 cgattcaggc gactattgaa ctggcacaaa aaccgatagt gctgattggc ggttgtaccg
    27121 gcagcggtaa aacgctgtta gtgcagcaac agccgaacgg tgttgatctg gaagggttgg
    27181 cgcgtcatcg cggttcggcg tttggtcgca cgttacaacc acaacttagc caggcgagtt
    27241 ttgaaaacct gctggctgcc gaaatgctaa aaaccgacgc ccgtcagaat ttgcgcctgt
    27301 gggtgctgga agacgaaagc cggatgatcg gttcgaatca cctgccggaa tgcctgcgcg
    27361 agcgaatgac tcaggcggcg attgcggtgg tagaagatcc gtttgagatc cgtcttgagc
    27421 gcctgaacga agagtatttc ttgcgtatgc atcatgattt tacccacgcg tacggcgacg
    27481 aacagggctg gcaggaatat tgcgaatacc tgcatcacgg actttcggct attaagcgtc
    27541 ggctggggct acagcgatat aacgaactgg ctgcaaggct ggatgcagca ctgacaacgc
    27601 aactcaccac cggcagcacc gacggtcatc tggcctggct ggtgccgtta cttgaagaat
    27661 attacgaccc gatgtatcgc tatcagcttg agaaaaaagc ggaaaaagtt gtctttcgcg
    27721 gtgagtgggc ggaagtggcg gaatgggtta aggcgcagta atgagggacg tatttgtatt
    27781 agtcgcgact tgatctgaca catggccttg aaaggttgag agttaccggt tttgatatga
    27841 gtgtcgaatc cttatccaaa acaagaggta actctcatgc ttcatactgc caatcccgtc
    27901 atcaaacaca aagccggttt gctcaatctg gctgaagaac tcagcaacgt atcaaaagcc
    27961 tgtaaaatca tgggcgtttc gcgtgatacg ttttatcgtt accgcgaact ggtcgctgaa
    28021 ggcggtgtgg atgcgcagat taatcgtagt cgccgcgctc ctaaccttaa gaaccgtacc
    28081 gatgaggcaa ctgaacaggc tgttgttgat tacgccgtcg ctttcccgac acatggtcag
    28141 caccgggcca gcaacgaact gcgtaaacag ggggttttta tctccgacag tggtgtccgt
    28201 tctgtctggt tactccataa ccttgagaat ctcaaaagac gatactgaac aaattttatc
    28261 aggtgacgtt ccgtaaaaag ttgtatggag ttctcgatgc agtcggcgcg actatcagta
    28321 cccccgctga accctggact atcgggccgc cgtactgggg catgtcaccc tggcgtgccg
    28381 ctggttttcc gctaatggtt tatttcctct ttctttcccg gcaagaattt attttaatcc
    28441 ggaaggatta tttattttca gatagggact cacattaaac acgtaacatt ttaattaatg
    28501 catggctgaa tatgtattac acaatgtcag atatcgtaaa agaccagtgc aattctatgt
    28561 aaactctctg actgttcatt ttatttgttg tttcagggtc ggaactcacc ctcaataaac
    28621 aactgaatac gagtagatat tcatcgtctg agctatatgg ctttacacaa tagccgacat
    28681 gtcttttctc agtcaggaat tttacttttc ctcactacga agttgacgac ttcgatatgg
    28741 gatagactct taattcaagc aattactctg cacaagcatc aggcaatcca gctccaatat
    28801 ttgttataaa ataatgcgta acattaattt caccaacaaa aaaaggttta caccatcccc
    28861 ttgccctggt atcttaccat atttacacca cacttttttt gtatctggat cattctttga
    28921 tacgattttc gccacttgtt ccctggccct cactgtcaat gttttcttaa acctcccccc
    28981 atcatcattt gttagggaaa aataagaaaa aaataatcat cttcta
//
LOCUS       DAZFDC010000053        28836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFDC010000053 DAZFDC010000000
VERSION     DAZFDC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.053"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(124..1092)
                     /gene="astE"
                     /locus_tag="ACHJ48_002404"
     CDS             complement(124..1092)
                     /gene="astE"
                     /locus_tag="ACHJ48_002404"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO4707954.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFKKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1085..2428)
                     /gene="astB"
                     /locus_tag="ACHJ48_002405"
     CDS             complement(1085..2428)
                     /gene="astB"
                     /locus_tag="ACHJ48_002405"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO4707955.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTQEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(2425..3903)
                     /gene="astD"
                     /locus_tag="ACHJ48_002406"
     CDS             complement(2425..3903)
                     /gene="astD"
                     /locus_tag="ACHJ48_002406"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO4707956.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKGELTAIIARETGKPRWEAATELTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGCETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRVLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVFTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(3900..4934)
                     /gene="astA"
                     /locus_tag="ACHJ48_002407"
     CDS             complement(3900..4934)
                     /gene="astA"
                     /locus_tag="ACHJ48_002407"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO4707957.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(4931..6151)
                     /gene="astC"
                     /locus_tag="ACHJ48_002408"
     CDS             complement(4931..6151)
                     /gene="astC"
                     /locus_tag="ACHJ48_002408"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO4707958.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNESVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFNEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            6596..7402
                     /gene="xthA"
                     /locus_tag="ACHJ48_002409"
     CDS             6596..7402
                     /gene="xthA"
                     /locus_tag="ACHJ48_002409"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO4707959.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7569..8279
                     /gene="ydjX"
                     /locus_tag="ACHJ48_002410"
     CDS             7569..8279
                     /gene="ydjX"
                     /locus_tag="ACHJ48_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO4707960.1"
                     /translation="MNAERKFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            8284..8961
                     /gene="ydjY"
                     /locus_tag="ACHJ48_002411"
     CDS             8284..8961
                     /gene="ydjY"
                     /locus_tag="ACHJ48_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO4707961.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPANNTLAT
                     VTFKITE"
     gene            8976..9683
                     /gene="ydjZ"
                     /locus_tag="ACHJ48_002412"
     CDS             8976..9683
                     /gene="ydjZ"
                     /locus_tag="ACHJ48_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFO4707962.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGSLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            9683..10231
                     /gene="ynjA"
                     /locus_tag="ACHJ48_002413"
     CDS             9683..10231
                     /gene="ynjA"
                     /locus_tag="ACHJ48_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO4707963.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQELTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10241..11407
                     /gene="ynjB"
                     /locus_tag="ACHJ48_002414"
     CDS             10241..11407
                     /gene="ynjB"
                     /locus_tag="ACHJ48_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4707964.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALNEAPNDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTFRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            11380..12915
                     /gene="ynjC"
                     /locus_tag="ACHJ48_002415"
     CDS             11380..12915
                     /gene="ynjC"
                     /locus_tag="ACHJ48_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO4707965.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTSVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAALALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            12915..13568
                     /gene="ynjD"
                     /locus_tag="ACHJ48_002416"
     CDS             12915..13568
                     /gene="ynjD"
                     /locus_tag="ACHJ48_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4707966.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            13635..14942
                     /gene="ynjE"
                     /locus_tag="ACHJ48_002417"
     CDS             13635..14942
                     /gene="ynjE"
                     /locus_tag="ACHJ48_002417"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO4707967.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRSSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAARGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(14951..15571)
                     /gene="ynjF"
                     /locus_tag="ACHJ48_002418"
     CDS             complement(14951..15571)
                     /gene="ynjF"
                     /locus_tag="ACHJ48_002418"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4707968.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKYQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            15658..16065
                     /gene="nudG"
                     /locus_tag="ACHJ48_002419"
     CDS             15658..16065
                     /gene="nudG"
                     /locus_tag="ACHJ48_002419"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO4707969.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(16031..16303)
                     /gene="ynjH"
                     /locus_tag="ACHJ48_002420"
     CDS             complement(16031..16303)
                     /gene="ynjH"
                     /locus_tag="ACHJ48_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO4707970.1"
                     /translation="MSRALFAVVLAFPLIALANPHYGPDVEVNVPPEVVSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            16539..17882
                     /gene="gdhA"
                     /locus_tag="ACHJ48_002421"
     CDS             16539..17882
                     /gene="gdhA"
                     /locus_tag="ACHJ48_002421"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO4707971.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKSSRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(17999..19039)
                     /gene="ynjI"
                     /locus_tag="ACHJ48_002422"
     CDS             complement(17999..19039)
                     /gene="ynjI"
                     /locus_tag="ACHJ48_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4707972.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKTMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEEQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(19167..21128)
                     /gene="topB"
                     /locus_tag="ACHJ48_002423"
     CDS             complement(19167..21128)
                     /gene="topB"
                     /locus_tag="ACHJ48_002423"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO4707973.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRYLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLISRQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLIKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(21133..22176)
                     /gene="selD"
                     /locus_tag="ACHJ48_002424"
     CDS             complement(21133..22176)
                     /gene="selD"
                     /locus_tag="ACHJ48_002424"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO4707974.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTVQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(22293..22844)
                     /gene="ydjA"
                     /locus_tag="ACHJ48_002425"
     CDS             complement(22293..22844)
                     /gene="ydjA"
                     /locus_tag="ACHJ48_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO4707975.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            23005..24861
                     /gene="sppA"
                     /locus_tag="ACHJ48_002426"
     CDS             23005..24861
                     /gene="sppA"
                     /locus_tag="ACHJ48_002426"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO4707976.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKADKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKAKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            24977..25687
                     /locus_tag="ACHJ48_002427"
     CDS             24977..25687
                     /locus_tag="ACHJ48_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFO4707977.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNVPGELFPKLEEAQKKIVAELNAQGINEKEIILGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATDGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            25819..26835
                     /gene="ansA"
                     /locus_tag="ACHJ48_002428"
     CDS             25819..26835
                     /gene="ansA"
                     /locus_tag="ACHJ48_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO4707978.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            26846..27487
                     /gene="pncA"
                     /locus_tag="ACHJ48_002429"
     CDS             26846..27487
                     /gene="pncA"
                     /locus_tag="ACHJ48_002429"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO4707979.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(27580..>28836)
                     /gene="ydjE"
                     /locus_tag="ACHJ48_002430"
     CDS             complement(27580..>28836)
                     /gene="ydjE"
                     /locus_tag="ACHJ48_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4707980.1"
                     /translation="GFLSYSGNVVLAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGG
                     FIGDYFGRRRAFRINLLIVGIAATGAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFT
                     EFIPATVRGKWSARLSFVGNWSPMLSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSG
                     KYFIESPRWLAGKGQIAGAECQLREVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFW
                     LLFKGEMLRRTLVAITVLIAMNISLYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAP
                     VGIFIAALIIDHFPRRLFGSTLLIIIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVC
                     FASAVYIPELWPTHLRLRGSGFVNAVGRIVAVFTPYGVAALLTHYGSITVFMVLGVML
                     LLCALVLSIFGIETRKVSLEEISEVN"
BASE COUNT         6957 a         7716 c         7320 g         6843 t
ORIGIN      
        1 gcagagatta tgaagaacta taccggatga ctggtgataa ataaagcaaa taaccaggat
       61 taatctgtat taatttataa gaaggtaact taatacccgc agaaagattt ctgcgggtaa
      121 gtattagctt attttttcga gcattaatcc cgcgcgtaat cccagcgcta ccaacggatt
      181 agggaataac acatactcta catcatgggt tacggtaaaa cgttcctcac cgtcctgcgc
      241 cagcaacgtt cctttcttaa acggcataaa attcagcgtg tcacttgcca tatgcatttc
      301 gaaggacggc gagtggcgag taatttgcga aaccacccga taacggagcg gcggtgttct
      361 cacgataccg acactctcac cagatagcag cgcagcgatt gcgctggcag ttactgcaaa
      421 ctggcgaaga tcgttttgcc caaagggcaa cgctttgcca agttccagcg tacaggccag
      481 cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca ccaggctcct gatggaacac
      541 cagcgcctcc agccccgccg cacccagcca cgtcaggaat ttctcgtccc agggaatgtc
      601 gcgttgcggt aatacaccga actgcggatg caaggagcca cgaattgcgg tatgtagatc
      661 aaggtgccag cgcacagatt ctttgccctg gtcataaaaa tcttccaggc actgttccag
      721 ttcgcgcgcc cgacaggttt ccccgctttc agcaaacagc tgccaacgac cgccaaacat
      781 tcggttcata tcgctatggc aataacgttt cccttgcttc agcgcaggag gattcccgag
      841 gatcaccagc aaccgccacc gtaacgggat ctcgccgtga gatatcgcgc caagcaatgc
      901 atcaagcatc tccacaggtg ccgtctcatt accgtggatc cccgcagaaa tcaccagagc
      961 gccttgcggt ggcgttaatg gcgtcagttc cagcacacca tcgcccagcc agcgccagcg
     1021 aacgccgttg atttctcgct cggtgatgac aggtttttta ctcgttaagg tcagagcaag
     1081 aaaattatcc attgccccct ccctcgcgct ggaacggata aaccgaaccg agattcagta
     1141 attggctcaa tacatccagt gcttcccgcc cttcgcgcag caattgcggg tcggccagat
     1201 cggcagcagt aagacgatcg cggtagtaac gatccaccca gtcattgagc gcattaaaca
     1261 aggtatcgtt catcatcacc gccggattca ccgcccggcg ttcttcttgt gtcaatacca
     1321 cccgcaaccg caggcacgcc gggccaccgc cattcgccat gctttcacgt aaatcaaaga
     1381 cttttagttc gctaatcggg ttgtcagcgg caaggagttc attgagataa ccccatactc
     1441 cggcgtgttc ccgacactcc tgaggcagca ccaacatcat ggaaccatca tcgcggctca
     1501 acagttggct gttaaacaga tatgtagaca ccgcatccga cacggaaacc tgagttgccg
     1561 gaacttctat cgccataaaa ccgttcaccc gcgcacgcag gtttgccagt aactgtgact
     1621 ggcgagcgaa cgcctgttgg tggcaaaaca gcacctggcg gttactcacg gcaatcacgt
     1681 cattatgaaa aacgccctgg tcgataacgt ccgggttttg ctgggcgaaa atcacctgtt
     1741 ggggattcac ctgattcagc cttgccaccg cctcgctggc ttcgcgagtc tgtcgcgccg
     1801 gataacggga aggccgggta tcattgccct cttctcgccc gtagacaaaa agttgcatac
     1861 ccggttcacc gtaatgaccg ccgagacgat tgtggtttgc cgccccctca tcaccgagca
     1921 aggctacctg cggcaacgcc gaatggacac taaatttctc ttcgtcgtta aaaatcgctt
     1981 ttaacagcga ttcagtgacg ggcgcttcca gcgaacggtg aaatttattg ttcaggttag
     2041 caacggtgag atgcactttg ccatccagcg tatcggcaga tggcgcgatc gttgccgcat
     2101 tggctaccca cattggcgaa gcggaactga cgctggaaag ccagtgcggt gcctggcgta
     2161 caaccttttc cagtacctgc tcatcgctac cactgaatcc caactgacgc agcaccggaa
     2221 taaacggacg ctcgtgaggc gggatcacgg cctgggggaa tcccgcatcg gcaagggttt
     2281 tcattttcag taagccctgt tttgccgcca gtcgcgggtt agagacctga aaacggtgac
     2341 gggtagaggc tttattacca aacgacaggc ccgcgtaatg atgcgtcagc cctaccagcc
     2401 cgtcgaaatt aacttcccag gcgttcatcg caccacctca tcggaaaaat ccagcccggg
     2461 gttaagcgtt gcgggcaaag ttaacgagtc cgactccagg ctcgccatag gccatgcgca
     2521 gtaatctgcg gcataccagg cgctggggcg atggttaccg gatgcaccaa tgccgccgaa
     2581 tggcgcggta ctggcagcac cagtaagcgg tttgttccag ttaacaatcc ccgcccgcgc
     2641 ctccagcaac agttgatcga atttttcccg ctcgggggaa accagaccgc aagagagtcc
     2701 gaagcgagtg ttattcgcca ttcgaatcgc ttcctcgaaa gaatcataac gccagacgcg
     2761 caataacggt ccgaacacct cttcatctgg tacgccagca acgcctgtca tttcaatgat
     2821 ccccggcgtc agcaacgatg tccctgcttg taataagcgc ggcgcaagca gggttcgtcc
     2881 acccatcgct tccagttgct gccaggcagt aaacacctgc tgtgcggcct gttcagaaat
     2941 cagcccgcca ataaacggct gcggttcgtc atcccagttg cccggcgtta atcgctggct
     3001 gacggcaacc agacgagcaa gaaatgcatc gccctgcgcc ccgcttttca gcaatacacg
     3061 gcgggcgcag gtgcagcgtt gcccggctgt aacaaacgcc gactgaatgg tcagatggac
     3121 agccgcgtcg atatccgcca cctcatcgat aatcagcggg ttattgccgc ccatctcaag
     3181 ggcgagaatt ttctccggct gaccggagag ctggcgatgc agctggtagc cggtattggc
     3241 gctgccggta aacagcaagc cgtcgagatc ctccagcgca ctcagcgcct gacccgtttc
     3301 acacccgccc tgcaccaggt tcagcacgcc tggcggcaag ccagcctgct gccataaacg
     3361 cattaccgct tcgccactcc acggtgtcag ttcgctgggt ttaaagataa tggtgttacc
     3421 tgccagcaat gcgggaacga tatgtccgtt cggcaaatga ccagggaaat tatacggccc
     3481 aaacaccgcc agcacgccgt gcgggcgatg tcgcaggctc gccgcaccgt ccggcatttc
     3541 actacgctgc tcgccggtac gaacgtgata cgccttaatt gatatcgcga ttttattgat
     3601 catcgccgtc aattcggttg ccgcctccca gcgcggcttg ccggtttctc gggcaatgat
     3661 tgcggttaat tcgcctttat tcctttccag caatccggca aagcgttcga caacggcctg
     3721 acgttcagca aatgagagcc gcgcccagcg cggaaacgcc gcacgggctg cccgacaagc
     3781 ctgcccgacc tgagcggcat cggcatcatt gccttgccat aacacctcgc ccgataccgg
     3841 attacgcttc acacgcgatg caccctggcc cgttatccag tcaccgttaa tccataaagt
     3901 catgctgttt tctcctctgc gcacaggcgc accagacgaa cgcgatcccc tgcatggcat
     3961 ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct cggttgccgg atcggtacgc
     4021 accagcacca cgcggaaatg gtgataattt tcattggcga ccaggcaggc cggaaaatcg
     4081 ccctgcgcag gctgcccttc tgccacttcc accagccgac ttttacggat ggcgcgcacg
     4141 cggtcgatgt cacactcaag cgtcggccca ccgtcgaaga tgtcgatata gttgcggtag
     4201 cgaaaacctt ctttctccag caccgcgcgg gcaggcgcgg tttgcggatg tacctgaccg
     4261 atgacgtcct gggcttcctg ggataaaaag tgcgtataga tcggatgttt cggcatcagt
     4321 tctgcaataa atgccttttg cccggtgccg cagagaaaat cggcgcggct aaaatccatc
     4381 gaaaagaagc gtttaccgag gctttgccag aacggtgaat agccgtgttc gtcaatcacc
     4441 ccgcgcattt cagcaaccac tttgtcatta aacttgtcgc gaaaagccgc cataaacata
     4501 aagcgcgatt tcgacagcaa atagccgttg ccctctttgc gccagtccgg gtcgagaaac
     4561 agcgtgcaca gctcgctgct gccggtgtga tcgttactga gaaacaacgt cggcaatgcg
     4621 ttatagacat tcagctcttt tgaggcgtga accaacgtgc cgacgcgata gttgtaccag
     4681 ggatcgttca gcccaactgc cacctcaatg gcgcaaatcc ccgccacggt gcctgtctcg
     4741 ctatcttcca gtacaaacac ataaccctgc tcacttttgg gcagttcacc ttgccaggtt
     4801 ttgattgccc tttcgatacg cgccgaaagc gtggcttcat tggcgggaag cgacgtcagg
     4861 ccgccgcccg ttttgctggc aagctgcatc agcgccgaga catctgagcg ctcaacggga
     4921 cggatgacca tcatgatgaa cctcggctaa caaagtgttc gcaagcaact gcaaagcgat
     4981 ccagtccggt cgtcacctct tcttcgctga cattgagcgc aggcgcaaaa cgcaccacgt
     5041 tgccacccgc aatcagtacc atcacgcctg ctttcgccgc ttcctgagag atctgtttcg
     5101 cttgcccggc gtaatcggca ttcagtacac agccaatcag caaacctaag ccgcgaactt
     5161 cattgaacaa accatagcgg tgattaataa tattaagacg ctcaacaaac cagtcgtgac
     5221 gttgcttaac cccgttaagc atctctggcg tattgatgag ctccagcact ttgcctgcca
     5281 ccgccgaggc cagcggattg ccgccatagg tggtgccatg agtgccaacg gtcatcacgc
     5341 tggcgcactc ttcggttgct aacaacgcac cgaccgggaa accgccgccc agagctttgg
     5401 cggtagttaa cagatcaggc gtcacgccgt agtgcatata ggcatacagt tccccggtac
     5461 gcccgacgcc ggtttgtact tcatcaaaaa tcaacagcgc attgtggcga tcacacaatt
     5521 cacgcagacc ttgtaaaaat gcgttgctgg ctggcaccac accgccttca ccctggatgg
     5581 gttcgacaat caccgcacag gtagagtcgt caatcagcgc gctggcagag ttaatatcgt
     5641 tatatgcagc atgacggata tctggtggca gtggcgcaaa atcctgtgaa taggctggct
     5701 gcccacccgc actaacagta aacagcgtgc gaccatgaaa cgcattttta aacgccacga
     5761 taccgctctt atggctgccg tagcggtcgt gagcgaattt acgcgccagt tttagcgccg
     5821 cttcgttggc ttccgcaccg gagttacaaa agaaaacgcg atcggcaaac gtggcgtcga
     5881 tcaatttttt cgccagtcgc agtaccgact cgttggtgta accgttgccg gtatgccaga
     5941 acttactcgc ctgttcgttc agcgcctcac gcagttccgg atgcgcatgg cccagcgcgt
     6001 tcaccgcaat gccacccgcg aagtcgatat actctttccc ctgctgatcc cacaagcgcg
     6061 aaccttcgcc acgtaccggt ataaacggtg ccggagcgta aacaggtatc atccattcat
     6121 caaagttttc acgcgtaatt ggctgagaca tagcgacctc tacagtaaat agctcgttat
     6181 ttatatgtta ataataagta atgttttcgc tgtaaatgta gattgcaggg ttcgtgccag
     6241 ccagtgatga aagtgcataa acggcggagg ctaactggaa atcaaggagt tataaccaaa
     6301 ccatatgcat ttaaagtgca tattaagtga atacgtttgc gatgtgggtg aataaaaaga
     6361 ataaaaaacg caatgttatg cagaagtaaa atataattct ggaattgtga tcattgacga
     6421 aatttactgg aaattactgc gccattctga cgcagcgcgc accaaaatcg gcattttttg
     6481 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     6541 aagcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     6601 atttgtctct tttaatatca atggcctgcg cgccagaccc caccagcttg aagccatcgt
     6661 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     6721 tcccctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     6781 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     6841 tgacgaagaa gaggcgcagc ggcggattat tatggcggaa atcccctcgc cgctgggcaa
     6901 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     6961 cccggcaaag gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaagcg
     7021 tgaaaatccg gtgctgatta tgggcgatat gaatatcagt cctggcgacc tggatattgg
     7081 cattggcgaa gagaaccgta agcgctggct gcgtaccgga aaatgttctt tccttccgga
     7141 ggaacgcgaa tggatggaga gactgatgag ctggggattg gttgatacct tccgccatgc
     7201 gaatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7261 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7321 agaaaccggc atcgactatg aaatccgcag catggaaaaa ccgtccgatc acgcccccgt
     7381 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     7441 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     7501 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     7561 actccacgat gaacgctgag cgtaaatttc tttttgcctg tcttattttt gcgctggcca
     7621 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     7681 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     7741 ccaccctctt tctgttacca ggaagcatat tggtgatcgc gggaggaata gtttttggcc
     7801 cgttcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     7861 tgctggcgcg ctggatggga cgtgatttac tgctgaaata cgttggccat agccatacct
     7921 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     7981 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc acaatcgcct
     8041 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8101 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8161 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8221 atgtggatct gtctgcttcg cgccgcaacc cacttactca cccaaaaaat gaaggataga
     8281 acgatgttgc aacattattc agtgtcatgg aaaaaaggac tgactgcact ctgcttactg
     8341 gctgttgctg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     8401 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     8461 ctggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     8521 gatggctcca acggacataa atcgctgttt atgggttatg cgaccccgaa agcattttat
     8581 gaagccctga aagaggcagg tggtacaccg ggcgaaaaca tgacgatgga taataaagaa
     8641 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     8701 acgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     8761 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     8821 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggttga aaaacgaggt
     8881 gaagttaaat tcaaaggcaa tgcctcggtt ctcccggcga ataacacgct ggcaacggtt
     8941 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9001 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctg gcatgggcgc
     9061 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9121 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9181 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9241 atgcgtcgct gtttggcgcg ttctggggcg gctcgctgtc gtggaccagt tcgatggccg
     9301 gcgcggcact gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
     9361 ccggcaaaac cgtacttgac agtatggacg gctttttcac tcgctacggc aaacacacca
     9421 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccggtt
     9481 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcag ttaccggcga
     9541 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg taccttctgg tttgtcaccg
     9601 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
     9661 agcgccagaa gaggaatgcc tgatgggttt accgccgctt agcaaaattc ctttcatttt
     9721 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc ccattcgctg
     9781 gtgggggcgg atcccgttta tcttttatct ggtgtcgatg tttgttggct ggctggagcg
     9841 caaacgctca ccgctcgatc cggtagtacg atcgcttgtc agcgcgcgca ttgcgcaaat
     9901 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gcaccggcag
     9961 cagcgataaa ctgctggcag tggctgactg gcggcaaagc ccgctcttta gcgatgaaga
    10021 acggctggcg ctggagtacg ccgaagccgc aagcgtaacg ccgccaacgg tcgatgatgc
    10081 cctgcgtacc cgactggctg cgcattttga cgctcaggag ctcaccgaac tgacggcatt
    10141 gatcggcctg caaaatctgt cagcccgttt taattctgcc atggacattc ccgctcaggg
    10201 gctgtgccgt attccggaaa aacgttctta aggagagatg atgcgccatt gtgggtggtt
    10261 gctgggattg ttatcgctgt tttctctggc aacacatgcc agtgactggc aagaaattaa
    10321 aaatgaggcc aaagggcaaa ctgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    10381 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    10441 tgttcgtctg gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    10501 acgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    10561 aaaagaggcc aatttactgc aaaccgactg ggcagagact ctgcccaact ggcgctatgt
    10621 cgacacacag ctgccggtgc gggaagattt ttcagtgcct acagaagggg ctgaatcgcc
    10681 ctgggggggc gcacaactga catttatcgc ccgccgcgat gttacgccac agccaccaca
    10741 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cctatccgcg
    10801 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgcccga
    10861 tcccgccgca ttaaatgaag cgccgaacga tgcgactttc gcccgtgtca ctgctccctt
    10921 gtggcaatat cttgatgcgc tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    10981 ttcgcccgcg cggatggatg ctctgctgaa agccggaaca tttcgcctgt cgctgacctt
    11041 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgcctgcaa gcagttacag
    11101 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc ctgccaacgc
    11161 gaatgccagt gctgcggcga aggtagttgc caatttcttg ctctcacccg atgcgcaatt
    11221 gcgtaaagca gatcccgctg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11281 tgatgggcag cgcgaaacat tgcaatcaag aatgccgcaa gatctgccgc cggtactggc
    11341 tgaaccgcac gcaggttggg taaatgcgct ggaacaagaa tggctacacc gttacggtac
    11401 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    11461 gctctcacgc tgatatcgcc tgctttgtcg ttgacacact ggcaggcgtt atttgccgat
    11521 ccacagttac cgcaggcatt actggcaacg ctggtgtcga caaccatcgc ggcggtcggg
    11581 gcgttgttga ttgccctact ggtgattgtg gcgctgtggc ctgggccgaa atggcagcgt
    11641 atgtgcaccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    11701 cttctgctct ttgctgacgg agggctgctt tatgactatt tcccgtattt cactccgcca
    11761 atggacaaat tgggtattgg gctgggcttc acgctggcgg tgaaagaaag cgcatttctg
    11821 ctgtggatct tagcagcggt attgagtgaa aaacggcttt tacagcaagt catcgtgctg
    11881 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    11941 cctgcgctgg caatggcgat gctggcgatt gttgcctggt cgctgtcggt cgtagatgtg
    12001 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12061 actcagggcg acgccgatca acaaacgaaa ggcgcactcg ccagcctgct gctgatgttg
    12121 ttactcgccg cctacgtttt gctgggctat ctgctatggt gtacctggcg gcgtactatt
    12181 ccccgcgtag atggcgttcg taagcctgcc acgcctttat tgccaggcat tacgctggcg
    12241 agttttttac ccttgaccag tgtgctgtgt gtggttctgc tggcgatcct cgcggatcag
    12301 tcgacgatca atagtgaagc gctcatcaac agcctgacaa tggggctggt ggcgacattc
    12361 atcgctttgc tcctgttact gctgtggctg gaatgggggc cacagcgtcg ccagttgtgg
    12421 ctatggttgc cgattttatt accggcactg ccgctggtgg cgggccagta cacgctggcg
    12481 ctatggctga acatggatgg aagctggacg gcggtggtct gggggcatct gctgtgggta
    12541 atgccgtgga tgctgtttat cctgcaaccc gcctggcagc gcattgattc acggttaatt
    12601 ttgattgcac aaacactggg ctggtcgcgg gccaaaatct tcttttacgt gaaatgtccg
    12661 cttatgctac gccctacgct gattgccttc gcggtgggat tttcagtcag tattgcgcag
    12721 tatatgccga cgctgtggct gggcgcgggg cgttttccga cgctcaccac tgaggcggtg
    12781 gcattaagca gcggcggcag caacggtatt ctcgccgccc tggctttatg gcaactgcta
    12841 ttaccgctta ttatttttgc cctgaccgcg ttagtcgcaa aatgggtagg ttatgtcaga
    12901 caaggactcc gctaatgctc tgcgtgaaaa atgtttcgct acgtttacca gaaagttgct
    12961 tgctgacaaa cgttaacttt acggtggata aaggtgacat tgtcacgtta atggggccgt
    13021 ctggctgtgg aaaatccact ctgttttcat ggatgattgg tgcactggcc ggacagtttt
    13081 cttgtacagg tgagctatgg ctcaatgagc aacggattga catgctaccc accgcacagc
    13141 gtcagattgg cattcttttt caggatgcac tgttatttga ccagttcagt gtcgggcaaa
    13201 atttactgct ggcgctaccg gcgacactta aggggaatgc ccgacgtaat gccgtgaatg
    13261 atgcacttga gcgttcaggc cttgacggag ctttccatca ggatcctgct actttgtctg
    13321 gcggtcagcg agcgcgcgtt gctctgcttc gcgcccttct cgctcagcca aaagcgttgc
    13381 tactggatga gccattcagc cgtcttgatg tggctctgcg cgataatttt cgccagtggg
    13441 tgttcagcga agttcgcgaa ctggcgatcc ccgtcgttca ggtaacgcac gatctccagg
    13501 atgttcctgc tgatagttct gttctggata tggcgcagtg gtcagaaaat tacaacaaac
    13561 tgcgataacg caaagttttt ctcaatgcgt cagttcagaa tggcgcactc aaaactacaa
    13621 tgtcgggatt ttcgatgaaa cgtgtttctc aaatgaccgc gctggcaatg gctttagggc
    13681 tggcttgcgc ttcttcgtgg gccgctgaac tggcgaagcc tcttacactt gaccagcttc
    13741 aacaacaaaa tggcaaagcg atagatactc gctccagcgc gttttataac ggctggccac
    13801 aaaccttaaa tggcccttct ggtcatgaac ctgccgcctt aaacctctct gctagctggc
    13861 ttgacaaaat gagcaccgaa cagctcaacg cgtggatcaa gcaacataac ctgaaagccg
    13921 atgccccggt ggcgctgtac ggtaatggca aagatgtcga cgccgtcaaa acgcgactgc
    13981 aaaaagcagg ttttacgcat atctccattc tgagtgacgc gctaagcgaa ccttcccgtc
    14041 tacaaaaact gccgcacttt gagcagctgg tttatccgca atggctgcac gacctgcaac
    14101 aaggtaaaga ggttacggcg aaacctgccg gtgactggaa agtcattgaa gcggccaggg
    14161 gcgctcctaa gctttacctt atcagccata ttcccggtgc tgactacatc gataccaacg
    14221 aagtggaaag cgaaccgctg tggaacaaag tttctgatga acaactaaaa gcgatgctgg
    14281 caaaacacgg cattcgccat gacaccacgg tcattctgta cgggcgtgac gtatacgctg
    14341 cagcgcgtgt ggcgcagatt atgctttatg ccggcgtgaa agatgtgcgc ctgctggatg
    14401 gcggctggca aacctggtcc gacgcgggtc tgcccgttga gcgcggtacg ccaccgaaag
    14461 tgaaagcgga accggatttc ggcgtgaaga tcccggcaca accgcagttg atgctcgata
    14521 tggaacaggc ccgtggactg ctgcatcgcc aggatgcatc gctggtgagc attcgttcgt
    14581 ggccagaatt tatcggtacg accagcggtt acagctatat taaaccaaaa ggtgaaatag
    14641 ccggagcacg ttggggacac gctggtagcg actcgacgca tatggaagat ttccataatc
    14701 cggatggcac catgcgtagc gccgatgata ttaccgctat gtggaaagca tggaatatca
    14761 aaccagatca gcaagtttca ttctactgcg gcaccggctg gcgcgcgtcc gaaaccttta
    14821 tgtacgcacg agcaatgggc tggaataacg tctccgttta cgacggcggc tggtacgaat
    14881 ggagcagcga tccaaaaaat ccggtagcaa ccggtgaacg cggcccggac agcagcaaat
    14941 aacattgcaa ttactgacgc tggagcgact tcagcgtcag atacccactc cacacccgcg
    15001 taaaggtcgt catccagcac aacgcaccga agatccacgc aaaccacgca aaccatgcag
    15061 ggaataaaca tcccagcaca aacagtaaga tcgtttctgt gccttcggtt aatcccccca
    15121 gataataaaa cgatttatgc gcataaccgg gattatcaat ctgatatttc gccgccagcg
    15181 cagcaaaagc gagaaaactg ctacccgtgc cgataaacgc aaacagcagc cagccgcctg
    15241 ccagggcgtt ttgctgcggt gcggcgagga taaacccaaa aggtaccagc gcgtaaaaaa
    15301 gaaaatcgag agaaatatca agaaagccgc ccgcgtcggt aagccctctg cgccgcgcca
    15361 gcgcaccgtc cagaccatca agcagcctgt tcaacaaaat gacgacaagc gccgccagat
    15421 accagcccag cgccagaaaa ggcagtgcca gcacaccgat ggcaaatccc actaatgtta
    15481 aaccatctgg cgtaatgccc ggtttatcaa gaacccgcac acactgatgc aacaacggtt
    15541 taatccgggg atgaagatgg cggtctagca caggcactcc ttaaatataa agcctttctg
    15601 attgagcaac agtgcggata ttatggcatt tttcgcttat ctgcccgtgt gtaatttatg
    15661 aaaatgattg aagttgttgc cgccatcatt gaacgtgatg gcaaaatttt actcgcgcaa
    15721 cgccccgccc agagcgatca ggcgggatta tgggagtttg ccggtggtaa agtcgagccg
    15781 gatgaaagcc agcagcaggc gctggtgcgt gagttaaacg aagaattggg catcgaagca
    15841 actgtgggtg agtatgttgc cagccatcag cgagaagttt cgggccggat tatccatctt
    15901 catgcctggc acgtacccga cttccacggg acgttacagg cacatgaaca tcaggcgctg
    15961 gtctggtgct cacctgaaga ggcgctgcaa tatccgctgg ctcctgctga cattccatta
    16021 ttagaggcgt ttatggcttt acgcgccgcc agaccagcgg attagtgcta agggttttgt
    16081 catcccgctg gcattgcagc agtattcctt cggctttaat taccgcccct tcagaataat
    16141 ttttatcctg ataaacgcag cactgagtac agggctgcgc tgactgcccg cctgaactga
    16201 ccacttctgg cggtacgttt acctccacgt ccggaccata atgcgggtta gccagtgcaa
    16261 ttaatggaaa tgctaatact acggcgaaca atgctcgact cacagggaac tccttaacgt
    16321 tattgtctct gctactgata acggtagccg ggtggcaaaa ctttagcgtc tgagttatcg
    16381 catttggtta tgagattact ctcgttatta atttgctttc ctgggtcatt tttttcttgc
    16441 ttaccgtcac attcttgatg gtatagtcga aaactgcaaa agcacatgac ataaacaaca
    16501 taagcacaat cgtattaata tataagggtt ttatatctat ggatcagaca tattctctgg
    16561 agtcattcct caaccatgtc caaaagcgcg acccgaatca aaccgagttc gcgcaagccg
    16621 ttcgtgaagt aatgaccaca ctctggcctt ttcttgaaca aaatccaaaa tatcgccaga
    16681 tgtcattact ggagcgtctg gttgaaccgg agcgcgtgat ccagtttcgc gtggtatggg
    16741 ttgatgatcg caatcaggta caggtcaacc gtgcatggcg tgtgcagttc agctctgcca
    16801 tcggcccgta caaaggcggt atgcgcttcc atccgtcagt taacctttcc attcttaaat
    16861 tcctcggctt cgaacaaacc ttcaaaaatg ccctgactac tctgccgatg ggcggtggta
    16921 aaggcggcag cgatttcgac ccgaaaggga aaagcgaagg cgaagtgatg cgtttctgcc
    16981 aggcgctgat gactgaactg tatcgccacc tgggcgcgga taccgacgtt ccggcaggtg
    17041 atatcggggt tggtggtcgt gaagtcggct ttatggcggg gatgatgaaa aagctctcca
    17101 acaataccgc ctgcgtcttc accggtaagg gcctttcatt tggcggcagt cttattcgcc
    17161 cggaagctac cggctacggt ctggtttatt tcacagaagc aatgctaaaa cgccacggta
    17221 tgggttttga aggcatgcgc gtttccgttt ctggctccgg caacgtcgcc cagtacgcta
    17281 tcgaaaaagc gatggaattt ggtgctcgtg tgatcactgc gtcagactcc agcggcactg
    17341 tagttgatga aagcggattc acgaaagaga aactggcacg tcttatcgaa atcaaatcca
    17401 gccgcgatgg tcgagtggca gattacgcca aagaatttgg tctggtctat ctcgaaggcc
    17461 aacagccgtg gtctgtaccg gttgatatcg ccctgccttg cgctacccag aacgaactgg
    17521 atgttgacgc cgcgcatcag cttatcgcta atggcgttaa agccgtcgcc gaaggggcaa
    17581 atatgccgac caccatcgaa gcgactgaac tgttccagca ggcaggcgta ctatttgcac
    17641 cgggtaaagc ggctaatgct ggtggcgtcg ctacatcggg cctggaaatg gcacaaaacg
    17701 ctgcgcgcct gggctggaaa gccgagaaag ttgacgcacg tttgcatcac atcatgctgg
    17761 atatccacca tgcctgtgtt gagcatggtg gtgaaggtga gcaaaccaac tacgtgcagg
    17821 gcgcgaacat tgccggtttt gtgaaggttg ccgatgcgat gctggcgcag ggtgtgattt
    17881 aagttgtaaa tgcctgatgg cgctacgctt atcaggccta caaatgggca caattcattg
    17941 cagttacgct ctaatgtagg ccgggcaagc gcagcgcccc cggcaaaatt tcaggcgttt
    18001 atgagtattt aacggatgat gctccccagg gaacatttct tatgggccaa cggcatttct
    18061 tactgtagtg ctcccaaaac tgcttgtcgt aacgataaca agcttcaagt tcagcatccg
    18121 ttaactttct gcgatagcag cagatatgcc agtaaagaaa tcccattaga ctattttttt
    18181 gataatcttc ttcgctttcg aacaactcgt gcgcctttcg agaagcaagc attatataat
    18241 gccaggccag ttcttcttca attgtcccgt tttgaaaagc tgtgcttgat atcgagatca
    18301 tccatgataa ttccgccgcc catattagct tcgccgagga tttaccggag ctatgattag
    18361 cgcaatcaga gatatagtct gagggaaaaa cagcaaattt attcaacaag gcgataacct
    18421 gctctggggc ttcctccatg tttgctttaa aggtattggc tccatggtcg ccagaaagaa
    18481 aatgctccat taaggcacaa taactttcgc tatcttcgat accccattgt tcctctaaag
    18541 actcgcgtct tttacttatg atatcgatcg agtcaaaagg aagcacatga tattggaagg
    18601 tatctttgcc aggttcaggc tttcgcggcc agaactccag cgtttcagac cattgcttat
    18661 gatagaatcg ataaggtgcg atcaattgta gcgcctgtaa tttctcgata ctgagcggct
    18721 caataccttt agcctgataa taatgcagct gttctttttt tgctttaaaa ccggcccgaa
    18781 caataagccc catcataatt aatagataaa gaaaagagca tcccgcggta atcaggcctc
    18841 tttcattcaa accgttggat gttatcgctg cgaatacaaa cattacagcg acaacacatg
    18901 ttaaataaaa cccccactta caaagcagca tggtcttatt ttctttaatc atccgttcaa
    18961 aattactatt aaatatttcc cagccattaa aagaatactt ctcgctccca ggatggtttt
    19021 gtaataaaac ttttttcatc acattccctg ttattacata ctttagaggc gataatttta
    19081 tccatgcaaa aaaaatatcc aacgaatttt cttgatccgc tggatatttc agaatatgac
    19141 tcgaatagca cgaaagattc gctcgcttac gctatcgccc cgcttccggc ttcatctgct
    19201 ggcggacttt ttttcgcact acgtttacgc ggtgcagcct ttttcttatc agcactgcca
    19261 ccactgcccg gagccacaat gccgcgaaac tgccgcaccg gagtacgttt ggcttgatca
    19321 ataagctgat atagcgtccc caccagcggc tgcataaagt cctgatagcg acactgcttt
    19381 tcgctgattt gcgtcagcac cgattcccag tgcgcggtca tgtccggtcg cgtagccatt
    19441 tctggcagcg aatggaataa cgcttttccg gcgtcggtgg agtggatata gcgccctttt
    19501 ttgatgagga aaccacgctt gaacaacagt tcaataatcc cggcacgcgt tgcttctgtc
    19561 cccagaccat cggtcgcacg aaggatcttt ttcagatctt tgtcctgcac aaagcgcgcg
    19621 atcccggtca tcgccgaaag cagtgttgca tcggtaaaat ggcgcggcgg ctgggtttgc
    19681 cgctctacca cttcaccttt ttcacacagc aactcatcgc ctttcgccac cacaggcaat
    19741 ggcgtaccgt cgttttcttc atcgcgctct ttgctgccta acagcgtgcg ccagcctgct
    19801 tcagcaagaa aacgcgcttt agcgacaaat ttgcctttgg caatgtccag ttcgataaca
    19861 cacttgcgga acaccgcatc cgggcagaac tgcatcagat actgacggga aatcaggtta
    19921 tagaccttcg cttcgttctc cgtcaggttg atcgcagaac tccgtgcggt cggaatgatg
    19981 gcgtggtgcg catcgacctt tttgtcatcc caacagcggt tgcgtatatc tggatctacc
    20041 actggctgcg gcaacagatc tggtgcatga acgctgatgg cattcatcac cgcgtggcgt
    20101 ccggcaaaat gttcttctgg caaatagcga caatcggaac gcggataagt gattagcttg
    20161 tgcgtttcgt acagcttctg gcagatatca agcacgttct gcgcgctcag accaaagcgt
    20221 tttgccgctt caatctgcaa cgccgaaagc gaaaacggca gcggcgcgga ttctgattcc
    20281 cgtttatcgt tatagctggt gacaatagcc ggttgaccac taatgcggtt aaccacatgc
    20341 tccgccagtg gacgatgtaa caagcgccct tcttcatcct ggtacggttc acacgcttcg
    20401 ctcggttgcc agatagcggt aaaccgctca tcggcaggtg tcacgatatg tgctttgact
    20461 tcaaagaagt ctttcgccac gaagttttca atctcttcat cgcggcgcac caccagccca
    20521 agcacgggcg tctgcacgcg tcccacggaa agcacgccct gataaccggc attgcgaccg
    20581 agaatggtat acgcacgggt catattgatg ccgtacagcc agtcggcacg cgctcgcgcc
    20641 agcgcagaaa cgcacagcgg tacaaactca ctgttggaac gaagacggtc gatcgcccgc
    20701 tcaaccgcct gcgggttcag gtcgtttatc aagcaacgct gcacctgctg gcgcttttcc
    20761 ggtgccagtt gtagatagtc cagcacttca tccaccagca gttgcccttc gcgatccggg
    20821 tccccggcgt gaacgatttc gctggcttca tgcaggtacc gtttgatgac gttaagttgt
    20881 ttggtcacgg agggtcgggg ctgtaattgc cacttttccg ggacaatcgg caaatccgca
    20941 agattccagc gcgcatagcg gctgtcgtag gcgtctggct gcgcctgctc aagcaggtga
    21001 ccgatacacc aggtcaccac ctgaccatta ccgcactcga taaagccatc gcctttccgg
    21061 tgcggtttgg gcaggacatc agcaatggcg cgcgccagac tcggtttttc ggcaataaac
    21121 aaccgcattg aattaacgga tctcaaccat ggcacgaccg ccgcgtgctg gcaccagttc
    21181 gccaatcgcc gtcagttcaa tgccaaactc ggcggctgta gctttgacct cattttcggc
    21241 ttccggcatg accgccagca gcaaaccgcc agaagtttgc ggatcgcaca gcaaatcgcg
    21301 cacttcacgc ggcatttcac ccatcagatg accgtagctg gcaaagttac gttcggtgcc
    21361 gccaggcact gcacccaact taatgtactc ttcaaccccc ggcaatttcg ggatcgcgtc
    21421 atagtcaacg cgcgcctgca cacctgcgcc ctgacacatt tcgctcaaat ggcccagcag
    21481 gccaaagccc gttacgtcgg tcatcgcttt tacgccttca atgttggcaa aggacgcgcc
    21541 tgcgatgttc atccggcaca tcacttccgt cgccagtccc tgatgttctg gtttcagcag
    21601 tgattttttc tcagccgtgg taagaacgcc gatccccagc ggtttcgtca ggaacagttt
    21661 acatccggct tgtacggtac tgtttttctt cacccgctcg gtcggtacga tccccgttac
    21721 cgccagacca aaaatcggct ccggcgcatc gatggagtga ccgccagcca gcgcaatacc
    21781 cgcctgacgg catgcatagc gtccaccttc ggttacttcg cgggcaattt ccggggaaag
    21841 tttgttaatc ggccagccga ggatcgcaat tgccataatc ggtttgcccc ccatcgcgaa
    21901 gatatcgctg atggcgttag tcgccgcaat gcggccaaaa tcgaaaggat tatcaacgat
    21961 cggcataaag aagtcggtgg tactgataac gctggtgcca ttgcccagat cgtacaccgc
    22021 cgcatcgtcg cgggtttcat tacccacaag caaattcgga tcaacaaact tcgcctgctc
    22081 actgtgcagg atggtttcca acacttttgg ggaaatttta cagccgcaac cagctccgtg
    22141 gctgtattgg gtcaaacgaa tcgagttctc gctcatggac atctcctgtc aatgcaatcc
    22201 gggtatggta accctcattc cgtgaagtga taagtgagag tgtctgaatt cctgcgcctt
    22261 tgctcacaat ccagacagtt tcgcgacaat tatcagaaat aagtgacaaa cggcgtcggg
    22321 tccgggacgt ttatcgacgt agatgctttc agctgcggcg tgccgaggta gagaaaaccg
    22381 acaattttat cctgctcacg gcaaccgaat gcttcacgca ctaccggact ttcagttaat
    22441 gcgccactgc gccagatgcc gccaaacccc tgggcaacgg ctgccatttg catcgccatg
    22501 accgcgcatc cggcagacat ttcctgttcc catcgcggga ctttatgatt ttcttcgcat
    22561 ttcgccacca ccgtgatgat gagcggtgcg cggaacggcg cattacgggc tttgtcgata
    22621 gctttgtcat cactaccggc agcaatcgcc ccctgttcca gtacggcgct gaaacgctcg
    22681 cgcccttccc cttcaatcac aaaaaaatgc cacggttgca tggacttatg gtccggcgcg
    22741 cgcatacccg cacgcaggat gttttgcagt tgttcacccg ttggtgcggg ttcagccagg
    22801 cgggaggcgc tacggcgatt gatcaatagt tcgagtgcat ccatttgatt aactcctgtc
    22861 gtgatattta ttcacaaaat taacacgaga gtggattttg ttacagcaca gtccgcaatt
    22921 cctgctgaca agtgccggtt gggtcattac gataaccaca tctattgcgc ctgtgacagg
    22981 tgtgacctta agttgggaga atacatgcga accctttggc gatttattgc cggatttttt
    23041 aaatggacgt ggcgtctgct gaatttcgtc cgtgaaatgg tacttaacct gttctttatt
    23101 tttctcgtgc tggttggtgt ggggatctgg atgcaggtca gtggtggtga ttcgaaagaa
    23161 acggccagtc gtggtgcact gctgctggac atttctggtg tgatcgtcga taaacccgac
    23221 agttctcagc ggtttagtaa attaagccgc caactacttg gtgccagttc cgatcgtctg
    23281 caggaaaact cactgtttga tatcgtcaat accattcgcc aggcgaagga tgaccgcaat
    23341 atcaccggta tcgtgatgga tctgaaaaac ttcgcaggcg gcgaccaacc gtctatgcag
    23401 tacatcggca aagccctgaa agagtttcgt gacagcggga aaccggttta tgccgttggc
    23461 gagaactaca gccaggggca atattatctc gccagtttcg ccaataaaat ttggctgtca
    23521 ccgcaaggcg tggtggattt gcacggtttt gctaccaacg gtctgtacta caaatcgttg
    23581 ctggataagc tgaaagtttc cacccatgtg ttccgcgtgg gtacgtataa atctgccgtt
    23641 gaaccgttta ttcgtgatga catgtcaccg gcagcccgcg aagctgacag ccgctggatt
    23701 ggtgagctgt ggcaaaacta tctgaatact gttgccgcta accggcagat ccctgctcag
    23761 caggtattcc ctggcgcgca agggttgctt gagggtttaa ccaaaaccgg tggcgatacc
    23821 gcgaaatatg cactggaaaa caagctggtc gatgcactgg catcgagtgc ggaaatcgaa
    23881 aaagcactga ccaaagagtt cggctggagt aaggctgata aaaattatcg cgccatcagt
    23941 tattacgatt acgcattgaa aacgccggca gataccggtg acagcatcgg tgtcgtcttc
    24001 gctaatggcg caattatgga tggcgaggaa actcagggga atgttggcgg tgataccact
    24061 gcggcacaaa tccgcgacgc tcgccttgac ccgaaagtga aagcgattgt cctgcgtgtt
    24121 aatagcccag gcggcagcgt taccgcgtct gaagtgattc gcgctgaact ggcagcagcc
    24181 cgggcagcgg gtaagcctgt ggttgtatcg atgggcggca tggcggcatc tggtggttac
    24241 tggatttcca cgccagctaa ttacattgtg gctaacccca gcaccctgac cggttctatt
    24301 ggtatcttcg gcgtgatcac caccgtagaa aatagtctgg attcgattgg tgttcatact
    24361 gatggtgtct caacttcacc gctggcggat gtttctatca ccagggcact gccgccggaa
    24421 gcgcagcaga tgatgcagtt aagcattgag aatggctata aacgctttat cacgctggtt
    24481 gctgatgcgc gtcattcgac gccggagcag attgataaaa tcgcccaggg ccacgtctgg
    24541 accggtcagg acgcaaaagc taacgggctg gtcgatagtc tcggggattt cgatgatgcg
    24601 gtcgccaaag cagcagagct ggcaaaagcg aaacagtggc atctggaata ctacgttgat
    24661 gaaccgacct tcttcgacaa agtgatggac aacatgtctg gttctgtccg ggcaatgttg
    24721 ccagatgcgt tccaggccat gttacctgca ccgctggcct cggtagcctc taccgttaaa
    24781 agtgaaagcg acaagctggc ggcgtttaac gacccacaaa accgttatgc gttttgcctg
    24841 acctgcgcca acgtgcgtta aatcctgcaa aaatgtggcc gatatgctgt cggccacact
    24901 cctgaatcgg actgtctttc actccctctc tccactacat tcccttgctc atttagcatc
    24961 tgatatggag tcatcagtga acaaggcccc ctctttaatt gccgctatcg tcctcggact
    25021 ggggattagc gcctgtggtt attttgttgg cgatggtgtt aaacatttaa aaaccaacaa
    25081 ccgttatgtc aatgtgcgcg ggctttctga aaaagaagtt cgcgcagata cggcggaatt
    25141 aacaattgcg attaacttta agggtaatgt tcccggcgaa ctgttcccga aactggaaga
    25201 agcgcagaaa aaaattgttg ccgaactcaa tgctcagggg attaacgaga aagagataat
    25261 tctgggccaa tggacaagca agcgaacaga ttccttttat ctgaaagatg atcccacaat
    25321 gccacgctat aacgcagacg ggtcagtgac gataaaaacc cataatgttg ctgctgtcga
    25381 aaaagtcgtc gcgaaattaa acgaactgca agtcgctacg gatggcgcta tcgctgagag
    25441 caaagtcgcc tatcgcttta atggtattgg cgcactgcgc gctgagatga ttgctgccgc
    25501 caccaaagat gcacgcaacg ccgcactaca atttgcaacg gactcaggta gccaggtggg
    25561 gtcaattagc gatgcctcac aaggcgtatt ccagatcttt gccagtggca gtgatgaaga
    25621 tgatcctacg gcgattaata aaacggtacg cgtagtcacg accgtaacct atgcgttgca
    25681 ggattaacca cttttctccg gcgggacact aatccctccg tcctgccgga cttttctcat
    25741 atccacagag tatccaggtg cgctcgctgc ccctcacgta tatacttttg ctctttcgat
    25801 atcattcata tcaatatcat gcaaaagaaa tcaatttacg ttgcctacac gggcgggacc
    25861 atcgggatgc agcgttccga gcagggttat ataccggtgt caggtcatct acaacgccaa
    25921 ctggcgctga tgccggaatt ccatcgcccg gagatgccag atttcaccat tcatgaatat
    25981 acgccgctga tggattcttc agatatgacg ccggaagact ggcagcatat tgctgaagat
    26041 attaaagcgc actatgacga ctatgatggt tttgtcattc tgcacggcac cgacacgatg
    26101 gcgtataccg cctctgcgct gtcgttcatg ctcgaaaatc tcggtaaacc ggtcattgtg
    26161 acagggtcac aaatcccgct ggctgagtta cgctctgacg gacaaattaa tctgctgaat
    26221 gcgttgtacg ttgcggcgaa ttatccgatc aacgaagtaa cactcttttt taataaccga
    26281 ctgtatcgcg gcaaccgcac taccaaagcc catgccgatg gttttgatgc gtttgcctct
    26341 ccaaaccttc ctccgttact ggaagcaggt atccatattc gtcgtttgaa tacgccaccc
    26401 gccccgcacg gtgaagggga attgatcgta catccaatca ccccacaacc aattggcgta
    26461 gtgacgattt atccagggat ttctgctgac gtcgtgcgca attttctgcg ccaaccggtg
    26521 aaagcattga ttctgcgctc ctatggcgtg ggtaatgcgc cacaaaacaa agccttcctg
    26581 caggaattac aagaagccag cgatcgcggt attgtggtgg tcaacctgac acaatgtatg
    26641 tccggtaaag tgaatatggg tggttatgcc accggtaacg ccctcgccca tgctggcgtt
    26701 attggcggtg cagatatgac tgtagaagcc acgctaacca aactgcatta cctgcttagc
    26761 caggaactgg atactgaaac cattcgcaag gccatgagcc aaaacctgcg cggcgaactg
    26821 acgccggatg attaaggaga ctgcaatgcc ccctcgcgcc ctgttactgg tcgatttaca
    26881 aaatgatttc tgtgctggtg gcgcgctcgc cgtgccggaa ggtgacagta cggtggatgt
    26941 cgctaaccgc ctgattgact ggtgccagtc gcgcggtgaa gcggttatcg ccagtcagga
    27001 ctggcacccg gcgaatcacg gcagttttgc cagccagcac ggtgtagagc cttatacgcc
    27061 aggccaactc gacggtttgc cacaaacctt ctggccagat cactgtgtgc agaacagtga
    27121 aggcgcacaa ttacatccgt tactgaacca aaaagcgatc gcagcggtgt tccataaagg
    27181 cgaaaatcct ttagttgaca gttacagtgc cttttttgat aacggccgtc ggcagaaaac
    27241 ctctctcgat gactggttac gcgatcatga aatcgatgaa ttgatcgtta tgggcctggc
    27301 tactgactat tgcgtgaagt ttaccgtgct ggacgcgtta cagttaggtt ataaggtaaa
    27361 cgtgattacc gatggttgtc gtggcgtgaa tatccagccc caggacagtg cgcacgcgtt
    27421 tatggagatg tcagcagctg gggcaacgct atatacgctg gcagactggg aagagacgca
    27481 ggggtaattt tacgctggcc tacaattctg tactggcatt gtaggccaaa taaaacacgt
    27541 cagtggcaca tctggcaatt gatgccatca acgaaagatt taatttacct cagaaatctc
    27601 ttccaacgac accttccgcg tttcgatgcc aaaaatggag agaaccagcg cacagagcaa
    27661 taacatgaca ccaagtacca taaacaccgt gatcgaccca taatgtgtta ataatgccgc
    27721 aacgccatag ggcgtgaaga ctgcgacgat ccgtccgacg gcattaacga aacccgaacc
    27781 gcgcaggcgt aaatgcgttg gccaaagctc cgggatataa accgccgacg cgaagcaaac
    27841 atacatgtat aaaaagaaga tcatcaccag gccatagatt aaaatcgccc actctgtagt
    27901 ctgaattgaa tagatatagc ctaacactgc aataataata agtaaggtgg agccaaataa
    27961 ccgacgagga aaatgatcaa taattaatgc cgcaataaat attcctaccg gagcgccaat
    28021 cataataaca gcggtcatta atattgattt atcgacatca atgccggagt taacaaatat
    28081 ggtcggtatc catacggtga tggtataaag cgaaatgttc attgcaatta aaacagtaat
    28141 cgcgactaat gtacgtcgta acatttcacc tttaaacagg agccagaaag tacccttgat
    28201 tactttaacc ttgctgttgc tctgatacga agtaagcggg ggtaaacgaa tactcttctc
    28261 tctttcaatt tgctgctcta cttcacgaag ttggcattct gcacctgcga tttgcccttt
    28321 ccctgccagc catcgtggcg actcaataaa gtatttacct gagagaaacc aggctaacag
    28381 tatgccaata ccacccaaca gaaacattat ccgccaacta aaaaaagcga taaccaccac
    28441 acctatcgcc gcagacagca tgggcgacca gttaccaaca aatgagagcc gcgcggacca
    28501 ttttccacgc accgtcgcgg ggataaactc cgtaaatgag gcatagccaa ccataatcag
    28561 cgcccccatt cctgttccca tcaggaagcg aaagaagata agccagtaca tatcagggac
    28621 aaaagcggcc cctgttgcag caataccgac gatgagaaga tttatgcgaa acgccctgcg
    28681 ccgcccaaag taatcaccga tgaacccacc ggtaagagag ccgatgaaat aaccaaacat
    28741 taatgccgag gtaaaggcgg cattgaggaa attatttgac catccattgc ttaccagctt
    28801 tgctaagacg acattaccgg agtaactcaa aaaccc
//
LOCUS       DAZFDC010000054        28409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFDC010000054 DAZFDC010000000
VERSION     DAZFDC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.054"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..286)
                     /locus_tag="ACHJ48_002431"
     CDS             complement(<1..286)
                     /locus_tag="ACHJ48_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO4707981.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRD"
     gene            complement(894..2261)
                     /gene="rhlE"
                     /locus_tag="ACHJ48_002432"
     CDS             complement(894..2261)
                     /gene="rhlE"
                     /locus_tag="ACHJ48_002432"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO4707982.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRK
                     PAAAQ"
     gene            2490..3161
                     /gene="cecR"
                     /locus_tag="ACHJ48_002433"
     CDS             2490..3161
                     /gene="cecR"
                     /locus_tag="ACHJ48_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO4707983.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            3164..4159
                     /gene="hlyD"
                     /locus_tag="ACHJ48_002434"
     CDS             3164..4159
                     /gene="hlyD"
                     /locus_tag="ACHJ48_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO4707984.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            4152..5888
                     /gene="ybhF"
                     /locus_tag="ACHJ48_002435"
     CDS             4152..5888
                     /gene="ybhF"
                     /locus_tag="ACHJ48_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO4707985.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5881..7014
                     /gene="ybhS"
                     /locus_tag="ACHJ48_002436"
     CDS             5881..7014
                     /gene="ybhS"
                     /locus_tag="ACHJ48_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4707986.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            7025..8131
                     /gene="ybhR"
                     /locus_tag="ACHJ48_002437"
     CDS             7025..8131
                     /gene="ybhR"
                     /locus_tag="ACHJ48_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4707987.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(8093..8503)
                     /gene="ybhQ"
                     /locus_tag="ACHJ48_002438"
     CDS             complement(8093..8503)
                     /gene="ybhQ"
                     /locus_tag="ACHJ48_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO4707988.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            8636..9397
                     /gene="ybhP"
                     /locus_tag="ACHJ48_002439"
     CDS             8636..9397
                     /gene="ybhP"
                     /locus_tag="ACHJ48_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4707989.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            9394..10635
                     /gene="clsB"
                     /locus_tag="ACHJ48_002440"
     CDS             9394..10635
                     /gene="clsB"
                     /locus_tag="ACHJ48_002440"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO4707990.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            10635..11591
                     /gene="ybhN"
                     /locus_tag="ACHJ48_002441"
     CDS             10635..11591
                     /gene="ybhN"
                     /locus_tag="ACHJ48_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO4707991.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHVPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(11627..12340)
                     /gene="ybhM"
                     /locus_tag="ACHJ48_002442"
     CDS             complement(11627..12340)
                     /gene="ybhM"
                     /locus_tag="ACHJ48_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4707992.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLIKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIVVFSTAGAMFLISMLAGLLFNIDPGSHRFMIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIILHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(12548..13252)
                     /gene="ybhL"
                     /locus_tag="ACHJ48_002443"
     CDS             complement(12548..13252)
                     /gene="ybhL"
                     /locus_tag="ACHJ48_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO4707993.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDASNLRKYSILCALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(13388..13840)
                     /gene="moaE"
                     /locus_tag="ACHJ48_002444"
     CDS             complement(13388..13840)
                     /gene="moaE"
                     /locus_tag="ACHJ48_002444"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO4707994.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGLVTVIHRIGELWPGDEIVFIGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13842..14087)
                     /gene="moaD"
                     /locus_tag="ACHJ48_002445"
     CDS             complement(13842..14087)
                     /gene="moaD"
                     /locus_tag="ACHJ48_002445"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO4707995.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(14080..14565)
                     /gene="moaC"
                     /locus_tag="ACHJ48_002446"
     CDS             complement(14080..14565)
                     /gene="moaC"
                     /locus_tag="ACHJ48_002446"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO4707996.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(14568..15080)
                     /gene="moaB"
                     /locus_tag="ACHJ48_002447"
     CDS             complement(14568..15080)
                     /gene="moaB"
                     /locus_tag="ACHJ48_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO4707997.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(15102..16091)
                     /gene="moaA"
                     /locus_tag="ACHJ48_002448"
     CDS             complement(15102..16091)
                     /gene="moaA"
                     /locus_tag="ACHJ48_002448"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO4707998.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(16079..16223)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            16488..17396
                     /gene="yvcK"
                     /locus_tag="ACHJ48_002449"
     CDS             16488..17396
                     /gene="yvcK"
                     /locus_tag="ACHJ48_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO4707999.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(17587..19608)
                     /gene="uvrB"
                     /locus_tag="ACHJ48_002450"
     CDS             complement(17587..19608)
                     /gene="uvrB"
                     /locus_tag="ACHJ48_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO4708000.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(20187..20864)
                     /gene="bioD"
                     /locus_tag="ACHJ48_002451"
     CDS             complement(20187..20864)
                     /gene="bioD"
                     /locus_tag="ACHJ48_002451"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4708001.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRSIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGESPWLAENPENAATGK
                     YINLALL"
     gene            complement(20857..21612)
                     /gene="bioC"
                     /locus_tag="ACHJ48_002452"
     CDS             complement(20857..21612)
                     /gene="bioC"
                     /locus_tag="ACHJ48_002452"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO4708002.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYP
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(21599..22753)
                     /gene="bioF"
                     /locus_tag="ACHJ48_002453"
     CDS             complement(21599..22753)
                     /gene="bioF"
                     /locus_tag="ACHJ48_002453"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO4708003.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(22750..23790)
                     /gene="bioB"
                     /locus_tag="ACHJ48_002454"
     CDS             complement(22750..23790)
                     /gene="bioB"
                     /locus_tag="ACHJ48_002454"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO4708004.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMSYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23877..25166
                     /gene="bioA"
                     /locus_tag="ACHJ48_002455"
     CDS             23877..25166
                     /gene="bioA"
                     /locus_tag="ACHJ48_002455"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO4708005.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQHFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            25225..25701
                     /gene="ybhB"
                     /locus_tag="ACHJ48_002456"
     CDS             25225..25701
                     /gene="ybhB"
                     /locus_tag="ACHJ48_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO4708006.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            26019..26111
                     /locus_tag="ACHJ48_002457"
                     /pseudo
     CDS             26019..26111
                     /locus_tag="ACHJ48_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26208..26906)
                     /gene="nleD"
                     /locus_tag="ACHJ48_002458"
     CDS             complement(26208..26906)
                     /gene="nleD"
                     /locus_tag="ACHJ48_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFO4708007.1"
                     /translation="MRPTSLNLVLHQSSRSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRAESQKYSPLLLEEARTVGLGAFSEEVLSENKFHEEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(26968..27089)
                     /locus_tag="ACHJ48_002459"
                     /pseudo
     CDS             complement(26968..27089)
                     /locus_tag="ACHJ48_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(27137..28018)
                     /gene="nleH1-1"
                     /locus_tag="ACHJ48_002460"
     CDS             complement(27137..28018)
                     /gene="nleH1-1"
                     /locus_tag="ACHJ48_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HFO4708008.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFVDTTETNVLYDRAKNEFNPIDISSYNVSDRSWSESQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(28188..28343)
                     /locus_tag="ACHJ48_002461"
                     /pseudo
     CDS             complement(28188..28343)
                     /locus_tag="ACHJ48_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         6849 a         7485 c         7153 g         6922 t
ORIGIN      
        1 catcacgagg aattgcatca ttcatattta tagagatcac attttcatgt ctggattgcc
       61 cggttttaat cgtcatattg atatcatttg cgctgatctc cgaaatctca ctgacgaatt
      121 ttttaaatgc attatagcgc tggtggccag cattaaattt gccaaatgaa acataatatg
      181 cgaaatgttc aaggaaacca tgtaattcca gtaaattaac cgggtcagaa ccctgcacca
      241 tatttaataa catcgtcgca taaccattgc attttgaaca atccatacat cgctcctttg
      301 cacttgaatg atctgcgaat cataagcctg gcaaaatggg gagtcttaca gaaatcgctc
      361 atttttaact tttcgtactg caaattcagg atcaacctgg ttatgaaagt ttgctgctgt
      421 acggctctaa acagactggc aatcaatctt tctatcaata agctgaaatg agcgattttt
      481 gatagtttat tagaactact aaagatatac ggtaatgaaa cgactgcaca ctttattttg
      541 cttagggaga tagtcggata cactatagac aatgaaaata ataaaatcag ggacagaatc
      601 aagctaatac tgaatcacat atctcataat aatttaccag atgatgaaca aactctttat
      661 gcaatagcat gtttattcca gattttccca gaatttgttt atgaatatca accaatgtta
      721 ttaacaataa ttcaaattgc aaggagaaat aatttcattg ccattttaga tacactagat
      781 agatcataaa gcaaatttat taaaacaata aaatcagaca tgtcatgcat gtctgaccgt
      841 gttacctgaa taatgggcaa aagcctgtga tactcttttg cccttacatg gtattactgc
      901 gcagcggcag gtttacgcgg acggcgacgg cgttgttgtt cgcctgccgg tttggcatcg
      961 ccaagacgac gagatggttt ttctgcaggc ttcgcgctag ctgatttcgc accaccttct
     1021 ccacggcgtg gttgctgttg accgcgacca ccaccacctt gcccacgacc gccgccgcca
     1081 cgttgctggc gaccgttctg gatcggttcg gctttgattg acgggtccgg ctcataaccc
     1141 ggaatcgcaa tgcgcgggat ctcttttttc agcagttttt cgatatcacg cagcagtttg
     1201 tgttcatcaa cacacaccag cgacaatgct tcgccagttg ccgccgcacg gccagtacgc
     1261 ccgatacggt ggacataatc ttccggtacg tttggcagtt catagttgac gacgtgcggc
     1321 aactcttcga tatccagacc gcgcgcagcg atgtcagttg ccaccagtac acgaatatcg
     1381 cccgatttaa aatcggccag cgcacgggta cgcgcacctt gcgatttatt accgtggatc
     1441 gccgcactac ggatgccatc tttattaagc tgttcagcga gatggttagc gccatgtttg
     1501 gtacgggtaa acaccagcac ctgctgccag ttccctttcc caatcatgtg cgacagcaat
     1561 tcgcgcttgc gtttcttatc aacaaagtga acatgctggg tcacctgatc agacgcggta
     1621 ttgcggcgtg ccacttctat ttccagcggg ttgtgcaaca gtttttccgc cagggcttta
     1681 atatcgtcag agaaggtcgc ggagaacaac aggttctggc gcttcgcagg tagttttgtt
     1741 aacacgcgac ggatatcgtg gataaagccc atgtcgagca tgcggtcagc ttcatcgagg
     1801 acgaggattt caacctgatc cagcttcact gcattctgat gttccaggtc cagcaaacgt
     1861 cccggcgttg ccaccagcac atcaacgccg ccgcgcaact tcatcatttg tgggttaatg
     1921 ctgacaccgc caaacaccac cagcgaacga atgttcaggt atttgctgta atcacggacg
     1981 ttttcgccaa tctgcgccgc cagttcacgg gtcggggtaa gaatgagcgc acgtaccgga
     2041 cgacgccctt tggcgtgcgg ctggcgagtg atcaggtgtt gcaacagcgg cagcgtaaag
     2101 cccgctgttt tgccggtgcc ggtctgggcg ctagccatca ggtcgcggcc ttccagcacc
     2161 gcagggatcg cctgctgctg aataggggtg ggttcacggt aaccctgctc ggcaacggcg
     2221 cgcaggatat cagggcttaa acccaaagaa tcgaaagaca taactactcc gaaccgcccc
     2281 gaccgttacc ggtgtagttt tcagggagat actgaaaagg aaaatgacaa aaaccacaat
     2341 gtcatgaggg agcggagtgt agcagttttt gtgacgcagc gcataaatta tccctgtgac
     2401 tggcgtagct aaaattctta gccatactgg acaactccca tatggagtca taatcttaat
     2461 caatcatttg attaagaatg gactgtgcga tgaataatcc tgccatgaca atcaagggtg
     2521 aacaggcgaa aaaacagctg attgctgccg cactggcgca gtttggtgaa tatggaatga
     2581 acgccaccac ccgcgagata gccgcccagg ccgggcagaa tatcgctgcc atcacctact
     2641 acttcggttc gaaagaagat ttgtacctcg cctgcgccca gtggattgcc gattttattg
     2701 gcgagcagtt ccgtccgcat gccgaggaag ccgaacgctt gttcgcccaa ccacagcctg
     2761 atcgggctgc catccgtgaa ctgatccttc gcgcctgcag gaacatgatt aagctgctca
     2821 cccaggatga caccgtcaac ctcagcaagt ttatctcccg tgagcagctc tctcccacgg
     2881 cagcctacca cctggtgcac gaacaggtga ttagtccgct acacagccac ctgacacgcc
     2941 tgattgccgc ctggaccggc tgcgacgcca atgacacccg catgatcctt catacccatg
     3001 cgttgattgg tgagattctg gcgttccgtc ttggcaaaga aacaattctg ttacgtaccg
     3061 gctggaccgc gttcgatgaa gaaaagaccg aactgatcaa ccagacggtg acctgtcata
     3121 tcgacctgat tttgcaagga ttatcgcaaa ggagtttgta gtgatgaaaa aacctgtcgt
     3181 gatcggactg gcggtagtgg tacttgccgc cgtggttgcc ggaggctact ggtggtatca
     3241 aagccgccag gataacggcc tgacgctgta tggcaacgtg gatattcgta cggtgaatct
     3301 tagtttccgt gttggggggc gcgttgaatc gctggcggtg gacgaaggtg atgctatcaa
     3361 agcgggccag gtgctgggcg aactggatca caagccgtat gagattgccc tgatgcaggc
     3421 gaaagcgggc gtctcggtgg cacaggcgca gtatgacctg atgcttgccg ggtatcgcga
     3481 tgaagaaatc gctcaggccg ccgcagcggt gaaacaggcg caagccgcct atgactatgc
     3541 gcagaacttc tataaccgcc agcaaggact gtggaaaagc cgcaccattt cggcaaatga
     3601 cctggaaaat gcccgctcct cgcgcgacca ggcacaggca acgctgaaat cagcacagga
     3661 taaattgcgt cagtaccgtt ccggtaaccg tgaacaggac atcgctcagg cgaaagccag
     3721 cctcgaacag gcgcaggcgc aactggcgca ggcggagctg aatttacagg actcaacgtt
     3781 gatagcaccg tctgatggca cgctgttaac gcgcgcggtg gagccaggca cggttctcaa
     3841 tgaaggtggc acggtgttta ccgtttcact aacgcgtccg gtgtgggtgc gtgcttatgt
     3901 tgatgaacgg aatcttgacc aggcccagcc ggggcgcaaa gtgctgcttt ataccgatgg
     3961 tcgcccggac aagccctatc acgggcagat tggtttcgtt tcgccgactg ctgaatttac
     4021 cccgaaaacc gtcgaaacac cggatctgcg taccgacctc gtctatcgcc tgcgtattgt
     4081 ggtgaccgac gccgatgatg cgttacgcca gggaatgcca gtgacggtac aattcggtga
     4141 cgaggcagga catgaatgat gccgttatca cgctgaacgg cctggaaaaa cgctttccgg
     4201 gcatggacaa gcccgccgtc gcgccgctcg attgcaccat tcatgccggt tatgtgacgg
     4261 ggctggtagg gccagacggt gcgggtaaaa ccacgctgat gcggatgctg gcgggattac
     4321 tgaaacccga cagcggcaat gccacggtga ttggctttga tccgatcaaa aacgacggcg
     4381 cgctgcacgc cgtgctcggc tatatgccgc agaaattcgg cctgtatgaa gatctgacgg
     4441 tgatggagaa cctcaatctg tacgcggatt tgcgcagcgt caccggcgag gcacggaagc
     4501 agacttttgc tcgcctgctg gagtttacgt ctcttggccc gtttaccgga cgcctggcgg
     4561 gcaagctctc cggtgggatg aaacaaaaac tcggcctggc ctgtaccctg gtgggcgaac
     4621 cgaaagtgtt gctgctcgat gaacccggcg tcggcgttga ccctatctca cggcgcgaac
     4681 tgtggcagat ggtgcatgag ctggcgggcg aagggatgtt aatcctctgg agtacctcgt
     4741 atctcgacga agccgagcag tgccgtgacg tgttactgat gaacgaaggc gagttgctgt
     4801 atcagggaga accaaaagcc ctgacacaaa ccatggccgg acgcagcttt ctgatgacca
     4861 gtccacacga gggcaaccgc aaactgttgc aacgcgcctt gaaactgccg caggtcagcg
     4921 acggcatgat tcaggggaaa tcggtacgtc tgatcctcaa aaaagaggcc acaccagacg
     4981 atattcgcca tgccgacggg atgccggaaa tcaacatcaa cgaaactacg ccgcgttttg
     5041 aagatgcgtt tattgatttg ctgggcggtg ccggaacctc agaatcgccg ctgggtgcaa
     5101 tattgcatac ggtagaaggc acacccggcg agacggtgat cgaagcgaaa gaactgacca
     5161 agaaatttgg tgattttgcc gccaccgatc acgtcaactt tgccgttaaa cgcggggaga
     5221 tttttggttt gctggggcca aacggcgcgg gcaaatcgac cacctttaag atgatgtgcg
     5281 gtttgctggt gccgacttcc ggccaggcgc tggtgctggg gatggatctg aaagagagtt
     5341 ccggtaaagc gcgccagcat ctcggctata tggcgcaaaa attttcgctc tacggcaacc
     5401 tgacggtcga acagaattta cgctttttct ctggtgtgta tggcttacgc ggtcgggcgc
     5461 agaacgaaaa aatctcccgt atgagcgagg catttggcct gaaaagtatt gcctcacacg
     5521 ccaccgatga actgccatta ggttttaaac agcggctggc gctggcctgt tcgctgatgc
     5581 atgaaccgga tattctgttt ctcgacgaac cgacttccgg cgttgacccc ctcacccgcc
     5641 gtgaattctg gctgcacatc aacagcatgg tagagaaagg cgtcacggtg atggtcacca
     5701 cccactttat ggatgaagcg gaatattgcg accgcatcgg cctggtgtac cgcgggaaat
     5761 taatcgccag cggcacgccg gacgatttga aagcacagtc ggctaacgat gagcaacccg
     5821 atcccaccat ggagcaagcc tttattcagt tgatccacga ctgggataag gagcatagca
     5881 atgagtaacc cgatcctttc ctggcgtcgc gtgcgggcgc tgtgcgtcaa agagacgcgg
     5941 cagatcgttc gcgatccgag tagctggctg attgcggtag tgatcccgct gttgctgctg
     6001 tttatttttg gttatggcat taacctcgac tccagcaagc tgcgggtcgg gattttactg
     6061 gaacagcgta gcgaagcagc gctggatttc acccacacca tgaccggttc gccctacatc
     6121 gacgccacca tcagcgataa ccgtcaggaa ctgatcgcca aaatgcaggc ggggaaaatt
     6181 cgcggtctgg tggttattcc cgtggatttc gccgaacaga tggagcgcgc caacgctacc
     6241 gcaccgattc aggtgatcac cgacggcagt gagccgaata ccgctaactt tgtgcaggga
     6301 tatgtcgaag ggatctggca gatctggcaa atgcagcggg cggaggataa cgggcagacc
     6361 tttgagccgc ttattgatgt acaaacccgc tactggttta acccggcggc gattagccag
     6421 cactttatta tccccggagc ggtgaccatc atcatgacgg tcatcggcgc gattctcacc
     6481 tcgctggtgg tggcgcgaga atgggaacgc ggcactatgg aggctctgct ctctacggag
     6541 attacccgca cggaactgct gctgtgtaag ctgatccctt attactttct cgggatgctg
     6601 gcgatgttgc tgtgtatgct ggtgtcagtg tttattctcg gcgtgccgta tcgcggttcg
     6661 ctgctgattc tgttttttat ctccagcctg tttttactca gtaccctggg gatggggctg
     6721 ctgatttcca cgattacccg caaccagttc aatgccgctc aggtcgccct gaacgccgcg
     6781 ttcctgccgt cgattatgct ttccggcttt atttttcaga tcgacagtat gcccgcggtg
     6841 atccgcgcgg tgacgtacat tattcccgca cgttatttcg tcagcaccct gcaaagcctg
     6901 ttcctcgccg ggaatattcc agtggtgctg gtggtgaacg tactgttttt gatcgcttcg
     6961 gcggtgatgt ttatcggcct gacgtggctg aaaaccaaac gtcggctgga ttagggagaa
     7021 gagcatgttt catcgcttat ggacgttaat ccgcaaagag ttgcagtcgt tgctgcgcga
     7081 accacaaacc cgcgcgattc tgattttacc ggtgctgatt caggtgattc tgttcccgtt
     7141 cgccgccacg ctggaagtga ccaacgccac catcgccatc tacgatgaag ataacggcga
     7201 gcattcggtg gagctgaccc aacgttttgc ccgcgccagc gcctttacac atgtgctgct
     7261 gctgaaaagc ccacaggaga tccgcccgac catcgacaca caaaaggcgt tactactggt
     7321 gcgtttcccg gctgacttct cgcgcaaact ggataccttc cagaccgcgc cgttgcagtt
     7381 gatcctcgac gggcgtaact ccaacagtgc gcaaattgcc gccaactacc tgcaacagat
     7441 cgtcaaaaat tatcagcagg agctgctgga aggaaaaccg aaacctaaca acagcgagct
     7501 ggtggtacgc aactggtata acccgaatct cgactacaaa tggtttgtgg tgccgtcgct
     7561 gatcgccatg atcaccacta ttggcgtaat gatcgtcact tcactttccg tcgcccgcga
     7621 acgtgaacaa ggtacgctcg atcagctact ggtttcgccg ctcaccacct ggcagatctt
     7681 catcggcaaa gccgtaccgg cgttaattgt cgccaccttc caggccacca ttgtgctggc
     7741 gattggtatc tgggcgtatc aaatcccctt cgccggatcg ctggcgctgt tctactttac
     7801 gatggtgatt tatggtttat cgctggtggg attcggtctg ttgatttcat cactctgttc
     7861 aacacaacag caggcgttta tcggcgtgtt tgtctttatg atgcccgcca ttctcctttc
     7921 cggttacgtt tcgccggtgg aaaacatgcc agtatggctg caaaacctga cgtggattaa
     7981 tcctattcgc cactttacgg acattaccaa gcagatttat ttgaaggatg cgagtctgga
     8041 tattgtgtgg aatagtttgt ggccgctact ggtgataacg gccacgacag ggtcagcggc
     8101 gtacgcgatg tttagacgta aggtgatgta acttcttatc tttcgccagc aaagacacta
     8161 ccgccgggcc ggcaaggatt gccagccctg caattgccag caaaaagttg ttttccagta
     8221 cgcgggacaa cagccacagc acaatcagcg ctgcgccaaa ataccaggtc gtggtcagct
     8281 cttccagcac gtctgccacc tcacgccagc gttgctcggg gtggcgctga aacaccagca
     8341 tcatcactac cgtgacacaa tacagtgcgc ataatcccac gccgacgtga accagagtct
     8401 tactcatcca gcccaccacc agcagcgcca ctaccagtaa atgcaacata atctgccagc
     8461 aacttagacc cgttgcgaca cgaacacgtt gttgccactt catggcttct ctcctgaaga
     8521 atttatctct acttactcag agtaccgaac tttacggaaa attccgtaag accgcacctt
     8581 tttgtgacgc cgactacact atttcaatga gataagaggc aaagacagga aaattatgcc
     8641 cgatcaaaca caacaatttt cgttcaaggt gctcaccatc aatattcaca aaggctttac
     8701 cgcgtttaac cgacgcttca ttttgccgga acttcgcgat gccgtgcgta ccgtcagcgc
     8761 cgatattgtt tgcctgcagg aagtgatggg cgcgcacgaa gttcatccgc tgcatgtgga
     8821 aaactggccc gatacctcgc actacgagtt tctcgccgac actatgtgga gcgattttgc
     8881 ctacggtcgc aatgccgtat acccggaagg gcatcacggc aacgccgtac tgtcgcgtta
     8941 tcccattgaa cattatgaga atcgcgatgt ttcggtcgat ggtgcggaaa agcgcggcgt
     9001 gctctactgc cgcattgtgc cgccgatgac cggaaaagcg attcatgtga tgtgcgtaca
     9061 tctgggcctg cgtgaggcgc accgtcaggc gcagcttgcg atgctcgccg aatgggtgaa
     9121 tgagctaccg gacggcgaac cggtattggt ggcgggtgat ttcaatgact ggcggcaaaa
     9181 agctaatcat ccgttaaaag tgcaggccgg actggatgag atttttaccc gcgcccacgg
     9241 acgcccggcg cgcacgtttc cggtgcaatt tcctctacta cgactggaca ggatctacgt
     9301 caaaaatgcc agcgccagcg cgccaaccgc gttgccgctg cggacatggc gacacctttc
     9361 tgatcatgcc cctttaagtg cggagattca tttatgaaat gtagctggcg cgaaggcaat
     9421 aagatccagt tgctggaaaa cggcgagcaa tattatcccg cggtgtttaa ggcgattggc
     9481 gaggcacaag aacgcatcat tcttgaaacg tttatctggt ttgaggatga cgtcggcaaa
     9541 caactgcatg cggcactact ggcagcagcg caacgcgggg ttaaagcgga agtcttgctg
     9601 gatggctacg gttcgccgga tctcagcgat gagtttgtca atgaactgac ggcagctggc
     9661 gtagtgttcc gctactacga tccccgccct cgcctttttg gtatgcgcac caatgtgttt
     9721 cgccggatgc atcgcaaaat tgtggtaatc gacgcgcgta tcgcctttat tggcgggctg
     9781 aattactccg ccgagcatat gtccagctac ggtccagagg ctaaacagga ttacgcggta
     9841 cgccttgaag ggccgattgt cgaagatatt ctccagtttg agctggaaaa cctgcctgga
     9901 cagagcgcgg cacgacgctg gtggcgacgt catcacaaag cggaagagaa ccgccagccg
     9961 ggagaagcgc aggtattgct ggtctggcgc gataacgaag aacatcgcga tgatattgaa
    10021 cgtcactatc tgaaaatgct cactcaggcg cggcgagaag tcattatcgc caacgcctac
    10081 ttcttccccg gctatcgatt tttacacgcc ttgcgtaaag cggcacggcg cggggtgcgg
    10141 atcaaactga tcattcaggg cgaaccggat atgccgattg tcagagtcgg tgcgcgcttg
    10201 ctgtataact atctggttaa aggcggcgtt caggtgtttg agtaccgccg ccgcccgctc
    10261 cacggcaaag tggcattgat ggacgatcac tgggcgacag tagggtccag taatctcgat
    10321 ccgctcagtt tgtcactgaa tctcgaagca aatgtcatca tccacgatcg tcattttaac
    10381 cagacgctgc gcgataatct gaacggcatt attgccgcag attgtcagca ggtcgatgag
    10441 accatgctgc cgaaacgcac ctggtggaac ctaaccaaaa gcgtgctggc gttccacttt
    10501 ttacgccact tcccggcgct ggttggctgg cttccggcac acacgccacg tctggcgcag
    10561 gttgatcctc ccgcacaacc gacaatggaa acgcaggatc gggtagaaac tgaaaacacg
    10621 ggggtaaaac cctgatgagt aaatcacacc cgcgctggcg cttagcaaag aagatcctca
    10681 cctggctgtt ttttatcgcg gtgattgtgt tgctggtggt ctacgccaaa aaagtggact
    10741 gggaagaggt ctggaaggtc atccgcgact acaatcgcgt tgcgctgctt agtgcggtcg
    10801 ggctggtggt cgtcagctat ctgatttacg gctgctatga cctgctcgcc cgcttttact
    10861 gcggtcacaa actggcgaag cgccaggtga tgctggtgtc gtttatctgc tacgccttca
    10921 acctgacgct cagtacctgg gtcggcggca ttggtatgcg ctatcgtttg tactcccgtc
    10981 tgggattgcc gggcagcact attacgcgga ttttctcgct cagtattacc accaactggc
    11041 tgggctatat tttactggca gggattatct ttaccgcagg cgtggtggag ttgccggacc
    11101 actggtatgt cgatcaaact acgctgcgta ttctcggcat tggcttactg atgattatcg
    11161 cggtttattt gtggttttgc gctttcgcga agcaccgcca tatgaccatc aaagggcaaa
    11221 agctggtgct gccttcgtgg aaattcgccc tcgcccagat gctgatttcc agcgttaact
    11281 ggatggtcat gggggcgatt atctggctgt tacttggtca aagcgtgaac tatttctttg
    11341 tactgggcgt gttgctggtt agcagtattg ccggcgtcat cgtgcatgtt ccggcgggga
    11401 ttggtgtgct ggaagcggtg tttatcgcgc tactggctgg ggagcataca tccaaaggca
    11461 caattatcgc cgccctactc gcttaccgtg tgctgtatta ctttatcccg ctgctgctgg
    11521 cgctgatctg ttacctgttg ctggaaagcc aggcgaagaa gctacgggcg aaaaatgaag
    11581 cggcgatgtg aagtttagtt aagttcttta gtatgtgcat ttacgactaa tgaaaaaaac
    11641 gcgtatgcct ttgccagaca agcgttatag ctatcgcggc aagtatccca aagaagccga
    11701 taaaatagta atagagtgtt aatgcacctg taaaagcgca acgaaccgga ctccacaact
    11761 cttcactatg gcatttcccc gccaattcaa ggagcttatt tcgtccatgc aagataatgc
    11821 ctgaccataa cacaatcatt aagcaactta ttacccaaat gggccgttca ctcattaatg
    11881 cggcattcac gatgattacc agggccaacc ctgtcaacgt catcataatc ataaaacggt
    11941 gagaaccagg atcaatatta aataataaac cggccagcat actgattaaa aacatcgcgc
    12001 ccgcagtgct gaacacgacg ataatggcat atacaggaac taaacttgca acgacaatcg
    12061 ccaccaaaga gagtaaaaac agatatatta cgcctgcgat ggcggcattt atcttgttga
    12121 tatccttaat taagccatac atacaaagta ataataaccc tatggcgaca tacattggtg
    12181 gtgaatcata tttggttgcc gtaatttcga gatacttagc cccccaggcc agccctgcgg
    12241 ttgccaccaa acctaaagcg gtaatgagcc atataccgtt taatatcctg gtctcgtgct
    12301 gaaaatctaa tttattgctg ttttgcaagt atgattccat tcttttagtt gtccttatag
    12361 aaaaatatta atattcatca tgaataaatt taaacccacg catacaaaat acttttgtat
    12421 ttatggatac ggaaaactct tccgctcggc cataaggcgt attttatacc tggtacttgt
    12481 taagtgtcgt ttttagtaag aaaaaaaatc ccgacacatg gccgggatat tgatggtgaa
    12541 aaaagaatta acgacgattg ccaaaaattc gcagcaacat caggaacagg ttgatgaagt
    12601 ccagatacaa ggttaatgcg caaagaatgg aatatttgcg caggttcgac gcgtcgcggg
    12661 tatctatctg ctcacccata tttttcagtt tctgggtgtc atacgccgtc aatccgacaa
    12721 agacaatcac gccgatgtag gtgactgccc acatcaacgc ttcgcttttc agccagaagt
    12781 tgaccagcga tgccaacaca atgccgatta acgccataaa cagcatattg ccaaagccac
    12841 ttaaatcgcg cttcgtggtg taaccgtaca ggctcattgc gccgaacatc ccggcagtaa
    12901 cgacgaaggt actggcgata gaagcagcgg tataaacaat gaaaatactg gaaagcgtaa
    12961 gacccgtcag cgccgaataa agcataaaga gcatcgtcgt tacacctgcg ctcagctttt
    13021 gaatcatcgc tgataacaca ataaccaatg ctaattgcgc gatgatcaga ccgattaaaa
    13081 agacacggtt agtgaacaac agctccatca cggccgcgga attagccgca taccaggcaa
    13141 caaatgcggt cagcaataag ccaacggtca tccagccata gacttgcgcc atataagtct
    13201 gcaagccagc ccggggttgt acaattgaat cagaacgtgg gaatctgtcc atgacgatct
    13261 cctgaagata taaggaatat cttaaggata ctgcaaaatg atgaggctgt gcatcgacgc
    13321 agcgtaaacg catgtactga gcggtgaaat tgccggacgc agcggtgcct tatccggcta
    13381 acaaaaacta ccagcgtttt gccgcctgct gatcgctctc ccgagcttcg acccagcggt
    13441 cgccctccgg cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga taatccataa
    13501 taaactgccc ggcttcaaac gcactgctgc gatgcgcact ggtgacaccg ataaaaacga
    13561 tttcatcgcc cggccataat tccccgatgc ggtgaatcac cgtgacgagc cccagcggcc
    13621 agcggttacg cgcttcatcg acaatttctg ccagtgcttt ttcagtcatc cccggatagt
    13681 gttcgagggt taatgctttg acgctgtcgc ccaggttatg gttgcgcacc ttaccggtaa
    13741 aggtgactac cgcaccgtct tcgtcacgct ccgccagcca cgggtactct tctcctacgc
    13801 tgaacggctg cggaccaaca acaattttgg tttctgccat cttaacctcc ggttaccggc
    13861 gggaagaaag ctacttcgtc gccgtcagtc agcggatggt caaaactcac cagcgtctgg
    13921 ttgacggcag ccagtaattt gccatcttcc agcgccagcg cccagcgatc gctctgcgca
    13981 gccaggtgct ggcgtaacgc ttcaacggtt gggaaatccg cagccacttc ggttgcatct
    14041 gttcctacca actcgcgcac ctgggcgaaa aaaagaactt taatcatccg cttccacctt
    14101 aaagtcaccc gacttgccgc cgctcttcgc cagcaaacgt accggaccaa tcaccatatc
    14161 tttttgcacc gctttgcaca tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa
    14221 cgcttccatt tcgacaccgg ttttcccggt caggcggcat aaggtttcta tacgcacccg
    14281 attgtgctcc ggctcggcct gtaaattgac ttcaactttg ctgagcatca gcggatgaca
    14341 cagcgggatc agatcccagg tgcgttttgc cgcctgaata ccggcaatac gcgcagtggc
    14401 aaatacgtcg cctttgtggt ggcgaccatc aataatcatc gccagcgtct cgctgcgcat
    14461 ggtgacaaag gcttccgccc gcgcttcacg cacggtttcc gctttggcgg agacatccac
    14521 catgtgcgct tcgccagcgg cgttgatatg ggtcagttgc gacatactta tttcttcaaa
    14581 tgtggatgga aattacacgg acgcgtacgg gcatccagct gcggcgcgat gatattttcc
    14641 catgcggtac ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt tttgttagcg
    14701 acgcccgcta ccgcacgaga ttgcaacgtg gaagtgccaa tctcttcaaa cgacaacata
    14761 cggaacactt caccaaaacc ttcaacttca cggtcgaaca acggcagcaa tgcttcggga
    14821 gcctgatcac cttccgtcag gccagtcccc cccgtaatca acaccacttg tacatcgtcg
    14881 ctggcgatcc acgcagatac ctgagcgcga atagcgtagc ggttttcttt cacaatggct
    14941 ttatcgacaa cgtgatggcc cgcttcttgc gccgaatcgc gcaaatagtg accggaggta
    15001 tcgtcttctt caccgcgacg attagaaacc gtaagaatag caatacgggt cgggataaat
    15061 tcagtgctta cctgactcat ctgatctctc cttttgacgt tttagccgcc aatgtacgat
    15121 aagttttgcg taataccggt gttgttttga tgcaggaaat gggtctgttt cttctcccgc
    15181 agcgccgctg aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg
    15241 cgcaggttaa cgccgccttc accaaacagg cagagatgga gtttaccaat ggaggaaacg
    15301 cgcagacggt tgcaagtggc gcagaagtct ttttcatacg gcatgataag gccaatctct
    15361 ccggcgtaat ccggatgaca aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg
    15421 tggatccagc cgcgacgcag tagctcgtca cgcagaacct gaccagagat gtgatgctta
    15481 cggaaaagct cgctgccctc gcccgtttcc atcagttcga tgaaacgcag ctggataggg
    15541 cgatgctgga tccagttcag aaaggtgtcg agctggtgat gattcacatc acgcatcagc
    15601 acggtattga ctttgacctt ctcaaaaccg gcctcaaatg cagcatcgat ccctgccatc
    15661 acctgattga atttatcctg gccggtaatg gcatgaaact ggcgggcgtc cagactgtcg
    15721 acgctgacgt taatgccagt aagtcccgca tcgcgccagt tcgccacatc gcgttccaga
    15781 cggtaaccat tggtggtgac cgctatctgg cgaatagcgt cgttttctcg cacagcggcg
    15841 atgatatcgg taaagtcgcg gcgtaaagac ggctcaccgc ctgtcaggcg cactttttcg
    15901 gtgcccagac tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt cagaaagcct
    15961 ttattggtga cgccgctcgg tttgtagcca tccggcaggc agtaggtgca acgaaagtta
    16021 cacacatcgg taatcgacag gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt
    16081 tgtgaagcca tgtacacctt tccagatacg ggaggcgaag tcatttcttc cttcgccctg
    16141 gtggcaatct ttacgagcat tgtcacggcc aaagcaccat atcagttgac ccaggtgcag
    16201 aggctagagt gtttagtggt taagccgata ctagcgtgta aatgagattt ttaccatcca
    16261 cactttcgct atgtaatcat gtatatagcg tcctgatcgt gctatttcgc tacagaataa
    16321 gggaaaatgg cggttttact ttgatataca tcatcaggga ggcatagcgt gcatcgtcct
    16381 ttagaggtag aagaaaatag tttgtatcct caatattggc aggttaattg ctgtttcccc
    16441 gcaatttgcg ctactgtagc gcggataatc tgactccagg aaactatatg cgcaatcgta
    16501 cgctggctga tcttgatcgt gtcgttgctc tcggcggagg gcatggactg ggacgcgttc
    16561 tctcatcact ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc acggataatg
    16621 gtggctcgac ggggcgtatt cgccgttcag aaggcggcat tgcctggggc gatatgcgca
    16681 actgccttaa ccagctgata actgaaccca gcgtcgcttc agcaatgttt gaatatcgtt
    16741 tcggcggtaa tggcgaactt tccgggcaca accttggaaa cctgatgttg aaggcactgg
    16801 atcaccttag cgtgcgacct cttgaagcga tcaatctgat tcgaaatctg ctgaaagtgg
    16861 acgcacattt gattccgatg tcggagcatc ctgttgatct gatggcgatt gacgatcagg
    16921 ggcatgaagt ttacggcgag gtcaatatcg accagttaac tacgccgatt caagagttat
    16981 tgttaacgcc taatgtaccc gcaacgcgtg aggcggttca cgctatcaat gaagcggatc
    17041 tcatcattat tgggcctggc agtttttaca ccagcctgat gccaattctg ctgctgaagg
    17101 aaatcgcgca ggcattacgc cgcacgccag cgccgatggt ctatatcggc aatctggggc
    17161 gtgagttgag tttacctgcg gccaatttga agctggaaag caagctggca attatggagc
    17221 agtatgttgg taaaaaggtc attgatgcgg tcatcgtcgg gccaaaagtg gatgtctcgg
    17281 cggtgaaaga gcggattgtg attcaggagg tgctggaggc cagcgatatt ccgtatcgtc
    17341 atgaccgcca gttgttacat agcgcactgg aaaaggcgtt acaggcttta ggttaacgga
    17401 aatccgaagg aaaattccgg cttcctaatg aagacaaagt gcgcgttgtt tatgacggat
    17461 gcggcgtgaa cgccttatcc ggcctacaaa ccgcgctaat tcaatgtatt gcagaatcat
    17521 gtaggcctga taagcgtagc acatcaggca attttgcttt tgtcatctgt cttcttcgtt
    17581 atcctgtcac gaagccgcga taaacaattc acgcagctga tgcaactggt cacgaatttg
    17641 tgccgcttct tcgaactcca gattttgcgc gtgttgcatc attaaccctt ccagctcatg
    17701 gattttctgt tgcaacgctt taggcgacat atccatcggc acattatccg gctcaacaat
    17761 cggtcgcgat tttcctctgc ccttcgcttt ggttttagca atgttctgcc ccaacgccag
    17821 gatatcgacc actttcttgt tcaagccttg cggcgtaatg ccgtgttcct cgttgtactt
    17881 ctgctgtttc tcgcggcgac gttcggtttc gccaatcgct ttcgccattg acggggtgat
    17941 cttatcgccg tagagaatcg ctttaccgtt aacgttacgc gccgcacgac caatagtctg
    18001 gatcaacgaa cgttcagaac gcaggaagcc ttctttgtcg gcgtcgagga tcgccaccag
    18061 cgaaacctcc ggcatatcca gaccttcgcg cagtaagttg atccccacca gcacgtcgaa
    18121 ctcgcccaga cgcaagtcgc ggataatctc catacgttcg acggtgtcga tatctgagtg
    18181 aagataacgc acgcgctcac cgtgttcttc gagatattcg gtaagatctt ccgccattcg
    18241 cttggtcagt gtggtgacca gtacgcgttc gttaattgcc gctcgctgac gaatctccga
    18301 aagaagatca tcaacctgtg tcgccactgg ccgcacttcg ataatcggat caagcaatcc
    18361 ggttggacgc accacctgat ccaccacatc gccgccggat ttttccagct cataattacc
    18421 tggcgtcgcc gaaacataga tggtttgcgg cgctaatgct tcgaactctt caaatttcag
    18481 cggacggtta tccagcgctg atggcaggcg gaagccgtac tccaccagtg tctctttacg
    18541 cgcccggtca ccgcgataca tgccgccaat ttgtggaatg gtgacgtgag attcatcgac
    18601 caccagcagc ccatcggcag gcaggtaatc aaacagcgtc ggcggtggct cacccggtcc
    18661 acgaccggag aggaagcgcg agtagttttc aatccccgaa cagtagccca gctcgttcat
    18721 catctccaga tcaaactggg tacgctgggt cagccgctgc tcttccagca gtttgttgtt
    18781 ttccaatagc actttgcgtc tggcggccag ctcttctttg atctcctcca tcgcctgtac
    18841 gatgcgctcg cgcggtgtga cgtagtgcgt tttcgggtag atggtaaaac gtggaatggt
    18901 ggaaacaatc tgcccggtta gcgggtcaaa taacgacaat cgttccactt cctcgtcaaa
    18961 cagttccacg cgaagtgcaa tgtcatccga ttctgccggg aagatatcaa tcacctcgcc
    19021 acgaacacgg aaagtaccac gctggaatgc ttgatcatta cgagcgtatt gcagctccgc
    19081 cagtcggcgc agaatcgcgc gctgatcgat aatcataccg accgtgagat ggagcatcat
    19141 cttgagatat aaatcaggat cgcccagacc ataaatcgcg gaaacagacg cgaccacaac
    19201 aacatcacgc cgctccagca tcgctttggt ggcggacaaa cgcatctgtt cgatatgttc
    19261 gtttaccgag gcatctttct caatgaaggt gtcggaactc ggtacatagg cttctggctg
    19321 atagtagtcg tagtaggaga cgaaatattc caccgcgttt tccgggaaga actctttcat
    19381 ttcgccatac agctgggccg ccagcgtttt gttgggcgca agtaccatgg tagggcgctg
    19441 aaggtcagca atgacattgg caatggtgaa ggttttcccg gaaccagtca caccaagtaa
    19501 cgtctggtgc gccaggccat cttccagccc ctcttcgaga cgtcgaatcg cctctggctg
    19561 atcgccagaa ggtttaaaag cggaattcag tttgaacggt ttactcatga gtcgctacct
    19621 gaaggagttg ggcgggcagg tatgtaattt tactcgtcgt acttaattat gccaacaaat
    19681 tatactggat aaaaaaacag ttcatcacca taatatttct gttacagcgt aaactccgct
    19741 gtcaatcatg agtaaaattt actctgtggc gagataaaac tcggacctta ccgggttatc
    19801 cccaaagcaa cggctttttt aacatttgtc aagatgagtg atgtcagttt tatggcaaga
    19861 gatgcctgtt cagtgactgc cattgcaatt atctaaccag ttaaaaaata aaagatattt
    19921 ctttgagccg tctttaacgc caattcgcgt gcaagccgcg tattctctcg cttgcctcgt
    19981 gttttctaac tctaatacac atggttatcc acaggaatag tggataactg tctgcagccc
    20041 ctatcccccg ccgcctgggc aattcccaca ttcgccgcat gatgcggaag taaaattttt
    20101 ttcgtgatta ttttagaatt taattggtta aattgcagtc aatcgaagac gcgatctcgc
    20161 tcgcaattta accaaataca ggatggctac aacaaggcaa ggtttatgta ctttccggtt
    20221 gccgcatttt ctggattttc tgcaagccag gggctctctc ccagcagcgg cgcgggaatc
    20281 atgcgggtga gcgtggtcat atattcagcg tgacgttttc ccggaggcgt gacatcgttc
    20341 gccacccaac ccgccagagt cagcccggcg tgttgtatcg cctgtgccgt caacatcgcg
    20401 tgattaatac agccaagttt cacgccaacc accagtatta ccggcagttg ttcctgtgtt
    20461 acccaatccg caaaagtgaa agtgtcagaa agcggcgtaa accagccgcc agcgccttcc
    20521 actaacaccc agtcagcctg ttgttcaagc gcgcgtaatc cggagctcat taccgatgat
    20581 tctatcgacc tgccctcttg cgcgctgatg atgtgcggtg aggtgggttc tgcgaaggtg
    20641 taaggattta ccgttgcgta atccagctgc aggctgctgt tgcgctgtaa cgccagcgcg
    20701 tcactattac gtaaaccctc cggggtcttt tcgctgccag aggcgaccgg tttataacct
    20761 gccgtccgat agcctgctgc ctttgcggct tgtaaaagtg cacaactggc gacagttttc
    20821 cccacttcgg tatccgttcc ggtgacaaaa taacgtttac tcacgaacaa tcactcccaa
    20881 aaaaagatga tacgtcagag gatatcgccc ctgttgttgc ggccaggcca gttgcaatcg
    20941 ctgcaactgt gaacgcgtta atattcgctg gtcgcgccct tcatgaagat gcgtggcacc
    21001 gatgcctttc agcgaacgca tggcactgag cgcatcatca aaccatagtg tgatgggctg
    21061 aatacgatgt tgataatgca cgccgttcag cgactgttcg atttcatctg gcggtaaaaa
    21121 gcgattagca tgcgggcgct cgtccaccgc ctgccacgcc tgatgcagtt cgggtaacga
    21181 tccctgcacc agcgtggtaa acgcgaccac gcctccgggg cgcaccaccc gatacagctc
    21241 gcggagtgcc gttgataaat taccgcacca ctgcaccgcg agattgctcc atgcaagatc
    21301 gaacgtcgca gtcgctaacg gcagggattc gatatctccc gccagataat ggtctgcagc
    21361 atctttctgg cgggcttgcg caagcattgg cggcgagaga tctaacgccg tcacctgcgc
    21421 gtgacgctct cgccagtggc ggctcatcca gccaggccca caccccgcgt ccagtacgtg
    21481 ggggtattta cgctgtggga gcattgccag taagacatca gcactctggc gctgtaactc
    21541 tgcgtgttgc tcatagtgcg cggctgcccg accaaatgcc gctgcaatgg cttgtttatt
    21601 aaccgttgcc atgcagcacc tccagcagac ggtcgatatc ctgcatttca tgcgcggcgg
    21661 ttagcgttaa gcgcagtcgc gcagtaccag agggtacggt tggcgggcga atcgccgtga
    21721 cccagcagcc ttgctggcgc agtttttctg ccagttgtaa cgcacggctg ttatcaccga
    21781 cgatcaatgg ctggatggcg ctgcatgaat cagcaagcgt aaacggcaaa tcctgtactc
    21841 cggcacgaaa acgcgtaatg agtgccgcca gtttttcgcg ccgtgcatca ccctcatcac
    21901 tgcgaatgac cgccagtgac gcacgtaatg cctgcgcctg agcgggcggc atactggtgc
    21961 tgtagataag atggcgggcg aattgcagca gataatccgc caccgtactg gagcaaagca
    22021 ccgcagcccc gctgacgcca aatcctttgc caaaagtcac taccagcaat tctggtttta
    22081 ccttttgcaa ccagcaggtg ccgcgcccct gctccccgat aacgcccgtg ccgtgggcat
    22141 catcgaccat caaccagcca ttgtgctgtt gcgttacctg ctggatttcc gccagtggcg
    22201 cactatcgcc gtccatgctg aacacgcctt ctgtcaccac cagttgctgc cccggacagg
    22261 gggaagcaag cagtcgcgcc agatgagtga catcgttatg aacaaaacgg cgaagctgcg
    22321 acgggcttaa actggcagcc tccagcaatg aggcatggct aagccggtca gcgacaatgc
    22381 ggtcctcttt tgccatcatc gcggcaatga ccgcctgatt agcggcaaaa ccagagataa
    22441 atagcagtgc ccgcgaatag cccagccact cggccagttc ttcttccagc gcctgatgcg
    22501 caacgctata accgctgacg tgaccggaac cgccgctacc gacgccaaat tgctccgcac
    22561 cctgcttcca ggcacggata atttgcggat gatggcttaa acctaaataa tcgttactgg
    22621 aaaagttcag atactggcga tcgtccgcca ccagccagcg tccggctcct tgcgccactg
    22681 gataacggcg acgcagggca tcggcagcac gccgcgcatc gagcgccgcg ttgattttct
    22741 cctgccagct catagtgctg ccgcgttgta atattcgtca gtgtccgggg tcatcagtgc
    22801 ctgctccaga cgctgctgtt gttcgttatc ccctgccagc acggcagttt gctgcggatt
    22861 tagccccagt ttgcgaaaca gttgcaggtc tttatcttct tccggattgg gcgtggttag
    22921 cagtttgcaa ccgtagaaaa tcgagtttgc gcctgccata aagcacatcg cctgagtctg
    22981 ttcgttcatc tgctcgcgtc cggcagaaag gcgcacgtaa gaggtcggca tcatgatccg
    23041 cgctacggca atggtgcgaa taaaatcaaa ggcatcgaca tcatcgttat ctgccagtgg
    23101 agtacctttc accttcacca gcatgttgat tggcacgctt tccggcggcg tcggcaggtt
    23161 tgccagttgc agtaataatc cggcgcgatc ttttaccgtt tcgcctaaac ccacaatgcc
    23221 acctgagcag actttgatcc cggcatcgcg cactttttcc agcgtatcaa ggcgttcctg
    23281 ataagtacgt gtggtgatga tattgccgta aaactccggc gaggtgtcga ggttatggtt
    23341 gtagtaatcc agcccggcat tcgcaaggcg ctgcgcctga gattcactca acgtacccag
    23401 cgtcatacac gcctccagcc ccatcgcttt tactccctgc accatttgtt ccaggtatga
    23461 catatcgcgt tcgtggggat tcttccacgc cgcgcccata cagaagcgcg tcgatcctgc
    23521 cgctttcgct ttgcgcgccg actccagcac ctgttcaact tccatcaacc gctcggcttc
    23581 cagcccggtt ttgtagcgcg agctttgcgg gcagtatttg caatcttccg gacaagctcc
    23641 ggtcttaatc gacagcaagg tgctgacctg cacctgacga ggatcgaaat gctggcgatg
    23701 cacctgctgc gcttcaaaca gcagatccag caacggtttt tcaaataatt ctgtgacttg
    23761 cgacaatgtc cagcgtgggc ggtgagccat ggggcttctc caaaacgtgt tttttgttgt
    23821 taattcggtg tagacttgta aacctaaatc ttttcaattt ggtttacaag tcgattatga
    23881 caacggacga tcttgccttt gaccaacgcc atatctggca cccatacaca tccatgacct
    23941 ccccgctacc ggtttatccg gtggcgagcg ccgaaggttg cgagctaatt ttgtctgacg
    24001 gcagacgcct ggttgacggt atgtcgtcct ggtgggcggc gatccacggt tacaatcacc
    24061 cgcagcttaa tgcggcgatg aagtcgcaaa ttgatgccat gtcgcatgtg atgtttggcg
    24121 gtatcaccca tgcgccagcc attgagctgt gccgcaaact ggtggcaatg acgccgcaac
    24181 cgctggaatg cgtttttctc gcggactccg gttccgtagc ggtggaagtg gcgatgaaaa
    24241 tggcgttgca gtactggcaa gccaaaggcg aagcgcgcca gcattttctg accttccgca
    24301 atggttatca tggcgatacc tttggcgcga tgtcggtgtg cgatccggac aactcaatgc
    24361 acagcctgtg gaaaggctat ctgccagaaa acttgtttgc tcccgccccg caaagccgca
    24421 tggatggtga atgggatgag cgcgatatgg tgggctttgc ccgcctgatg gcggcgcatc
    24481 gtcatgaaat cgcagcggtg atcattgaac cgattgtcca gggcgcaggc gggatgcgca
    24541 tgtaccatcc ggaatggtta aaacgaatcc gcaaaatgtg cgatcgcgaa ggtatcttgc
    24601 tgattgccga cgagatcgcc accggatttg gtcgtaccgg caaactgttt gcctgtgaac
    24661 atgcagaaat cgcgccggac attttgtgcc tcggtaaagc cttaaccggc ggcacaatga
    24721 ccctttccgc cacactcacc acgcgcgagg ttgcagaaac catcagtaac ggcgaagccg
    24781 gctgctttat gcacgggcca acttttatgg gcaatccgct ggcctgtgcg gctgcaaacg
    24841 ccagcctggc gattctcgaa tctggcgact ggcagcaaca ggtggcggct attgaagtac
    24901 agctgcgcga gcaacttgcc cccgcccgtg atgccgaaat ggttgccgat gtgcgcgtgc
    24961 tgggggccat tggcgtagtc gaaacaactc gtccggtgaa tatggcggca ttgcaaaaat
    25021 tctttgtcga acagggtgtc tggatccggc cttttggcaa actgatttac ctgatgccgc
    25081 cctatattat tctcccgcaa cagttgcagc gtctgaccgc agcggttaac cgcgcggtac
    25141 aggatgaaac atttttttgc caataacggg aagtcagcgt gagagtttct ggctacactt
    25201 tctgcaaact agaaaggagg gtttatgaaa ctcatcagta acgatctgcg cgatggcgat
    25261 aagttgccgc atcgtcatgt ctttaacggc atgggttacg atggcgataa tatttcaccg
    25321 catctggcgt gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac ctgctacgac
    25381 ccggatgcgc caaccggctc cggctggtgg cactgggtag ttgttaattt acccgctgat
    25441 acccgcgtat taccgcaagg gtttggctct ggtctggtag caatgccaga cggcgttttg
    25501 cagacgcgta ccgattttgg taaaaccgga tacgatggcg cagcgccgcc gaaaggcgaa
    25561 acccatcgct acatttttac cgttcacgcg ctggatgtag agcgtattga tgtcgatgaa
    25621 ggagccagcg gcgcgatggt cgggtttaac gttcatttcc actctctggc aagcgcatcg
    25681 attaccgcga tgtttagtta atcactctgc cagatggcgc aatgctatct ggtatcactt
    25741 aaaggtatta aaaacaactt tttgtctttt taccttcccg tttcgctcaa gttagtataa
    25801 aaaagctgaa cgcgaaacag caaaacccaa taatatcaat gtattacaat gcatttagtc
    25861 taaaaaatag actgcataat gctacaaaac acaacatatc cagtcactat gaatcaacta
    25921 cttagatggt attagtgacc tgtaacagag cattagcgca aggtgatttt tgtcttcttg
    25981 cgctaatttt ttgtcaaaca cattacgtcg tttgctgaat ttacaaccat gctcagtatc
    26041 tctataagcg cagagaaatg atgcagtggt gggtgggctg gcttgatgaa aaggtggggt
    26101 actccacctg aaccactatc ggaatagcac aaagccttgc aatccagtgc aaagttttgt
    26161 gtgcctccgt ttgccccacc aatatatatt cagcacaaga aacacagcta aagcaatgga
    26221 tgcagtctta cctgttggaa tcccagactc actgtattgt catcatgaat aagagctgag
    26281 tcgtgcgggt aggaggttct acggggcatc ccaatctctt cgtggaattt attttctgaa
    26341 agcacctcct ctgaaaaagc ccccaaccca acagtcctgg cttcttcgag taaaagtgga
    26401 gagtattttt gtgattctgc tcgggaactc tcaactttca aacgctcccc atttaaattg
    26461 tggaaaacat ggagcaactc atgaaaaaca atagtcgcat gaaagtccac cacgctaatc
    26521 ccctccccac agggatattc aactgcattc agattacagt gaaaatcgga accagtcccc
    26581 cgatggttct cagcatctat atctctatgt gccataactc ctagtctgga agagttgagg
    26641 tgtattacca ctgtttctga tttaagtcgg gatattgatt caatagcatt caggagtaca
    26701 cgccctgtct ctgtagagcc aatcttatcg agtgctgctt caatctgctg atatatatta
    26761 tgatcagtcc cctggaaacg aagttgcgga ttatttttta tccattgaac gctcgatgct
    26821 tttacaagag actcgatatc tgtatctgac attgagctcg accttgatga ctgatgtaat
    26881 accaagttga gggacgtagg gcgcatcagg actcctgaga atgctactta ttagcagcct
    26941 gtttaccact ctgtgaaaat gattttttta aaagtgaccg tcaaggcggt caccaaaccc
    27001 gataataaag cggatcatag ccatttgcca gtcctcagcg acattgccca tttccgtgat
    27061 ccgccaagaa ttgccaacca cacaatttgt taccaactcc atccatgcaa caaatccact
    27121 acactggata aaattactaa attttactta ataccacact aataagatct tgctttccgc
    27181 catgataaga ttgcattatt tgactttcac tccatgaacg gtcggaaaca ttataagatg
    27241 atatatctat tggattaaac tcattcttcg cgcggtcata taagacattt gtctctgttg
    27301 tatcgacaaa aagaattcct ttttgctcca gtctatcaaa catatcgtaa atagcatgct
    27361 cagcctgtgc tggcaaggac gaaatattta aaagcgattc tccatttatt ttatccattc
    27421 taataccaat aatatcacca ttattgccat atattttttc tgcactcccg gcaccataat
    27481 attggttgaa gcaacgaact tcgcttgtca cctcttcatt gctttgagat gtagtaaaca
    27541 tcttcaggac ttttgttgca tcctcagcat cttcatatac tacagcgttc ccccctttgc
    27601 caataacatt accaggtact gacaacctgt tatagtccac actcggtaac tctggcggtg
    27661 cataatcaac aggaggtaaa tcaggtctgt tcgaatgaac aatgcctctt tccatagcag
    27721 cgctcactgg tgatgcattc agcatgacct caattttcct gctaatctcc cccttaggcc
    27781 atcccagcct gtgcaacaga ttagtaaaac aaccactatg actttctctt gtaacgcaaa
    27841 acttattatc ggtggcaaca acacgatatg ttctgttgcc aacctttact tgcgccccat
    27901 tatcagaatg aacggcagca tcccttacag aggataaaac acgattatca ggcgaagtca
    27961 ggtttctggt taaagaattc catgaacatc ccaaatttac agaatatggc gataacatac
    28021 atttcaacct tcaaaataaa cccatctaaa tttccttaac aaacatcccc cctaacatgg
    28081 caacaaaaat tagaaatgaa ctcttgcttt ttgtatctgg caactaattc atccttccag
    28141 catcccatca aacgaacgtt tacgaactat aacgcaggta ttaccggtca ctcgatatct
    28201 ggagatacag acgtatcaac atgattgagc ggcatacgat actgcttcca tgccgccagt
    28261 aacgatgtct gctcatccgt cgcaatatct aaatctgcag catcctgaag tggtgcgata
    28321 tactcactga ttacaagtac cagaccgata gattaaaaaa aacacaataa cacgctgcat
    28381 ttaaaacaaa aaattttaaa tatgattta
//
LOCUS       DAZFDC010000055        28335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFDC010000055 DAZFDC010000000
VERSION     DAZFDC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.055"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..614)
                     /gene="yncG"
                     /locus_tag="ACHJ48_002462"
     CDS             complement(<1..614)
                     /gene="yncG"
                     /locus_tag="ACHJ48_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO4708009.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYVWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPTLQEILKRNEI"
     gene            881..2380
                     /gene="ansP"
                     /locus_tag="ACHJ48_002463"
     CDS             881..2380
                     /gene="ansP"
                     /locus_tag="ACHJ48_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO4708010.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2495..3556)
                     /gene="yncE"
                     /locus_tag="ACHJ48_002464"
     CDS             complement(2495..3556)
                     /gene="yncE"
                     /locus_tag="ACHJ48_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO4708011.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHJ48_002465"
     CDS             3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHJ48_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO4708012.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGSVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGMEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHJ48_002466"
     CDS             complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHJ48_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO4708013.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHJ48_002467"
     CDS             complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHJ48_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO4708014.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQREMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8017..8535
                     /gene="mddA"
                     /locus_tag="ACHJ48_002468"
     CDS             8017..8535
                     /gene="mddA"
                     /locus_tag="ACHJ48_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO4708015.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8532..8981
                     /gene="ydcZ"
                     /locus_tag="ACHJ48_002469"
     CDS             8532..8981
                     /gene="ydcZ"
                     /locus_tag="ACHJ48_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4708016.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNSLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHJ48_002470"
     CDS             complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHJ48_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO4708017.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHJ48_002471"
     CDS             complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHJ48_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO4708018.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9669..9764
                     /gene="yncL"
                     /locus_tag="ACHJ48_002472"
     CDS             9669..9764
                     /gene="yncL"
                     /locus_tag="ACHJ48_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO4708019.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(9861..11285)
                     /gene="patD"
                     /locus_tag="ACHJ48_002473"
     CDS             complement(9861..11285)
                     /gene="patD"
                     /locus_tag="ACHJ48_002473"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO4708020.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTVACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11307..12101)
                     /gene="ydcV"
                     /locus_tag="ACHJ48_002474"
     CDS             complement(11307..12101)
                     /gene="ydcV"
                     /locus_tag="ACHJ48_002474"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4708021.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12091..13032)
                     /gene="ydcU"
                     /locus_tag="ACHJ48_002475"
     CDS             complement(12091..13032)
                     /gene="ydcU"
                     /locus_tag="ACHJ48_002475"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4708022.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(13033..14046)
                     /gene="ydcT"
                     /locus_tag="ACHJ48_002476"
     CDS             complement(13033..14046)
                     /gene="ydcT"
                     /locus_tag="ACHJ48_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4708023.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFICVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14064..15209)
                     /gene="ydcS"
                     /locus_tag="ACHJ48_002477"
     CDS             complement(14064..15209)
                     /gene="ydcS"
                     /locus_tag="ACHJ48_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO4708024.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQTNALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASSL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15454..16860)
                     /gene="ydcR"
                     /locus_tag="ACHJ48_002478"
     CDS             complement(15454..16860)
                     /gene="ydcR"
                     /locus_tag="ACHJ48_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4708025.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIACRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWLWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(16939..17355)
                     /gene="hicB"
                     /locus_tag="ACHJ48_002479"
     CDS             complement(16939..17355)
                     /gene="hicB"
                     /locus_tag="ACHJ48_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO4708026.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17401..17577)
                     /gene="hicA"
                     /locus_tag="ACHJ48_002480"
     CDS             complement(17401..17577)
                     /gene="hicA"
                     /locus_tag="ACHJ48_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO4708027.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            17799..18029
                     /gene="yncJ"
                     /locus_tag="ACHJ48_002481"
     CDS             17799..18029
                     /gene="yncJ"
                     /locus_tag="ACHJ48_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO4708028.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(18121..20082)
                     /gene="rlhA"
                     /locus_tag="ACHJ48_002482"
     CDS             complement(18121..20082)
                     /gene="rlhA"
                     /locus_tag="ACHJ48_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO4708029.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20155..20691)
                     /gene="sutR"
                     /locus_tag="ACHJ48_002483"
     CDS             complement(20155..20691)
                     /gene="sutR"
                     /locus_tag="ACHJ48_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310064.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO4708030.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVIISLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            20744..21955
                     /gene="ydcO"
                     /locus_tag="ACHJ48_002484"
     CDS             20744..21955
                     /gene="ydcO"
                     /locus_tag="ACHJ48_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO4708031.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
     gene            complement(21990..22658)
                     /gene="ydcL"
                     /locus_tag="ACHJ48_002485"
     CDS             complement(21990..22658)
                     /gene="ydcL"
                     /locus_tag="ACHJ48_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO4708032.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(22962..23555)
                     /gene="tehB"
                     /locus_tag="ACHJ48_002486"
     CDS             complement(22962..23555)
                     /gene="tehB"
                     /locus_tag="ACHJ48_002486"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO4708033.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(23552..24544)
                     /gene="tehA"
                     /locus_tag="ACHJ48_002487"
     CDS             complement(23552..24544)
                     /gene="tehA"
                     /locus_tag="ACHJ48_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO4708034.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            24668..25648
                     /gene="ydcK"
                     /locus_tag="ACHJ48_002488"
     CDS             24668..25648
                     /gene="ydcK"
                     /locus_tag="ACHJ48_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO4708035.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     INQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(25643..26179)
                     /gene="rimL"
                     /locus_tag="ACHJ48_002489"
                     /pseudo
     CDS             complement(25643..26179)
                     /gene="rimL"
                     /locus_tag="ACHJ48_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
     gene            complement(26242..26466)
                     /gene="ydcH"
                     /locus_tag="ACHJ48_002490"
     CDS             complement(26242..26466)
                     /gene="ydcH"
                     /locus_tag="ACHJ48_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO4708036.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDDRGY
                     NAEVVRMKKQKLQLKDEMLKILQHESVKEV"
     gene            complement(26606..28261)
                     /gene="opgD"
                     /locus_tag="ACHJ48_002491"
                     /gene_synonym="mdoD"
     CDS             complement(26606..28261)
                     /gene="opgD"
                     /locus_tag="ACHJ48_002491"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO4708037.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
BASE COUNT         7012 a         7225 c         6897 g         7201 t
ORIGIN      
        1 ataatttcat tcctttttag tatttcttgt aacgttggta actgacatac tgcatcagca
       61 atcgcactga tattcgtggc attatcctga aaccattcat gcccaggccc ccatgtgcgc
      121 atagtgcaaa gataacagtc caccagcgtt agttgctcac caaacgcata aggttcagcg
      181 gtcaattgtg aattcagcca gacataaagc gatttacgat attcaataac attcttcttt
      241 aactgttcag gtgcgtcaga tgcccaacgc tcagggtaat cggcgaaagt gaacgttgga
      301 tagacattag caaccagcca gaccaataac cgttgaaact gctgacgttc ggcacgccca
      361 accggtggcg caaggtccgg gcgacgatcg agaaccatca atgcaatcgc cgctgtctcc
      421 gtcataatct cgtcattttc cagtgccagt gtcggaacct gacacaacgg gttcagggtt
      481 ttcaataact cgcgtgaagt tccctcgtgg tcaaaaccac tgacatcaac gaactgataa
      541 ggaatatcag ccagtgttag catcagctca ctgattgtag agccccatcc gggtacgcca
      601 tacaccttaa tcatatgccc ctcacaatct tttaaagatt aaatgtagga cagtacaaag
      661 gttaaccagc aaaaggattt gtcatttttt cgtcagttgt attaatcccc ttcaggattg
      721 taaggggatg ttttgcatcg tttatcgata acgggtagga tgctgcgccg atagttttat
      781 acccataaag aataatggtg ataactatca tcgccaggat gaataaacat tgttcatggc
      841 aacttatatg actttttcat taaagcaatc agggagagca atgagtaaac acgacaccga
      901 cacttcagat caacacgccg cgaaacgccg ctggcttaat gcccacgaag aggggtatca
      961 caaagcgatg ggcaatcgcc aggtacagat gatcgccatt ggcggcgcga ttggcaccgg
     1021 cttgttttta ggtgcaggag cccgactgca aatggcgggt ccagcactgg cactggttta
     1081 tttaatttgt ggcttgtttt cgttttttat tctgcgtgca ttgggtgagc tggtgctaca
     1141 ccgcccttcc agtggcagtt ttgtctctta tgcccgtgag tttttgggtg agaaagccgc
     1201 ttatgttgct ggctggatgt acttcatcaa ctgggcgatg acggggattg ttgatattac
     1261 cgccgtcgct ctgtatatgc attactgggg tgcgtttggc ggcgtgccgc agtgggtctt
     1321 tgcgctcgct gcacttacca tcgttggcac catgaatatg atcggtgtga aatggtttgc
     1381 ggagatggag ttctggtttg cgcttattaa agtgctcgcc attgtgacct ttttggtcgt
     1441 gggtacggtg ttcctcggta gtggtcagcc gctggatggc aacaccactg gctttcattt
     1501 aatcaccgat aatggcggct tcttccccca cggtttgctg cctgcgctgg tgttgattca
     1561 gggcgtagtg tttgcttttg cctccattga aatggtgggt acagctgccg gagaatgtaa
     1621 agatccgcag accatggtgc ctaaagcgat taacagtgtg atttggcgta ttggcctgtt
     1681 ttacgtcggc tccgtggtgt tgctggttat gttattgccg tggagcgcgt atcaggcggg
     1741 gcaaagtccg ttcgtgacgt ttttctctaa actgggtgtg ccatatatcg gcagcattat
     1801 gaacattgtg gtgctgaccg ctgccctctc cagcctgaat tcaggtctgt actgcaccgg
     1861 acgtattctg cgctcaatgg cgatgggcgg ttccgcaccg agttttatgg cgaaaatgag
     1921 tcgtcagcat gtgccgtatg ccgggattct ggcgacactg gttgtgtatg tcgtcggcgt
     1981 attcctcaac tatctggtgc cgtcgcgcgt atttgagatt gtgttgaact tcgcgtcgct
     2041 gggaatcatc gcttcatggg cgtttatcat cgtgtgccag atgcgcctgc gtaaagcgat
     2101 taaagaaggc aaagcagcgg atgtcagttt taaactgcct ggcgcgccct tcacttcctg
     2161 gctgacatta ctgtttttac tgagtgtcct tgtgctgatg gcgttcgatt acccgaacgg
     2221 gacttatact atcgcggcgc tgccgattat cggtattctg ctggttatag gctggtttgg
     2281 tgtgcgcaaa cgcgttgctg aaattcacag cactgcgcca gtcgtcgagg aagatgaaga
     2341 aaaacaggaa attgtgttta agcctgaaac ggcgagctaa ttgcaaaatt gcccggctac
     2401 gtgccgggca aatttagatt actgacgaag gtgtgccgca tgcggcgtga tcaaagtcag
     2461 gcaagtctct ccgttatcac cctcgccata tctattacag cgcaatacga atcacatcgt
     2521 ctggctgggt agcttcctgc tgtttagtgg atttttgttt cacactgaca tacagcgttt
     2581 tgccatcggc agacagcgcc aggctgttcg gatgagtcgg cgtatcgaac gttttcacca
     2641 ctttatagct tttcgcgtca atcacactga ctttacctgc ctgacgatgc gttacgtagg
     2701 cttcattacg cgctgggtta aacagcacag ccagtgattc cggtgccgca accttcgcca
     2761 gaatattgcc attacgggta tcgaccacta acacttctgc cgctttagaa tcggtgataa
     2821 atgcacgctg cctggcggta tcaaggctga tgttgataaa gaagtgctct ttgccgtcat
     2881 ccagcagctt tttacggctg aggattttat tgtcggcggt gtcgatggta atcaattcgc
     2941 cgtcagcgtt agtggtgtaa agacgtttgc ctttgctatc cagtgccaga ccggtactca
     3001 ttttaccggt gttctggatg gcggttttca gtttgatatt ctcgccatca acgacccaaa
     3061 tcacgctatc tttaccaata ccactgatgt aaacggtgtt cgtggcatca tcagctacca
     3121 gctcacgcgg ttgcagcggg cgcacctctt ccgtgcgctt acgatcatcc agcaccagac
     3181 ggcctttcac ctcgccagtt ttggcatcta tcgccgtgac cgcgctgttt acggtgttac
     3241 caaaccacaa cgtctgagtc gtgttattga tggtggcacc aaacggcttg agatcgttat
     3301 ggatcgcctg cgtcacttcc agagtgaccg gatcaagacg ataaaccacg ccgcctttat
     3361 ccagtttgcg gctttgcgaa gtggcgagcc acagcgcgtt ttcttgctgg ctataagcca
     3421 tttcgtaggc acctttacct accgctttac gcagcatttc ttctgcggcc tgcgtactga
     3481 atgatgaagc aacaagcaat gaacctaaca gtaatgaacc acgcaggcgc aatgaaaaca
     3541 gatgacgtaa atgcatgacg actccctttg atgaaccgat atttttgctg cttcacaatg
     3601 cttcgctaag cgtaatcatc gttacgctct tgttataaat gggaatggta atcattattt
     3661 actcaaatgt ggagcatttc ttgccgttac gactacaatt ctctcaatta attaacgtat
     3721 cgtgccgttt cgttttccat aaatttacaa tttcattacc atgacggtgt tttcccattc
     3781 ttaacctggc ttttttcatg aagattgttt tcgttcgaca gaccgttttg cccgccctgc
     3841 ttgtcctttc ccccgttgtt tttgccgctg atgaacagac catgattgtc agtgccgcgc
     3901 cgcaggtggt ttcagaactg gatacccctg cagcagtaag cgtggtggat ggcgaggaga
     3961 tgcgcctggc aacaccgcgc attaacttgt ccgaatcact gaccggcgtg cctggtttgc
     4021 aggtacaaaa ccggcagaac tatgcgcaag atttacagct gtcgattcgt ggatttggct
     4081 cccgctccac ttacggaatt cgcggtattc gcctgtatgt ggacggtatt cccgctacca
     4141 tgcccgacgg gcaagggcaa acatccaaca tcgatttaag cagtgtgcaa aatgtggaag
     4201 tgctgcgtgg ccccttctct gccctgtatg gcaacgcgtc tggcggtgta atgaatgtca
     4261 ccacccagac cgggcaacag ccaccaacca ttgaagccag tagttactac ggcagttttg
     4321 gcagctggcg ctatgggctg aaagcaacgg gcgcaacggg agacggcaca cagcctggcg
     4381 atgttgatta caccgtctca accacgcgtt ttacgaccca cggctatcgt gaccatagtg
     4441 gcgcacagaa aaatttagct aatgccaaac tgggcgtacg cattgatgac gccagcaaat
     4501 taagcctgat tttcaatagc gtggatatca aagcagatga cccaggtggg ctaaccaaag
     4561 cagaatggaa agcgaatccg caacaagcgc ctcgtgcaga acagtacgac acgcgaaaaa
     4621 ccatcaagca aactcaggct gggttgcgct atgaacgtag cctgagttcg cgggatgata
     4681 tgagtgtgat gatgtatgcc ggagagcgag aaacgaccca gtaccagtca atacccatgg
     4741 caccacaact taacccgtca catgcgggca gcgtgattac cctgcaacgc cattaccagg
     4801 gaatagacag ccgctggaca caccgtggtg aactgggcgt tccggtcacg ttcactaccg
     4861 gcctgaacta cgaaaacatg agtgaaaacc gcaagggcta caataacttc cgcctgaata
     4921 gcggcatgcc ggagtacggg caaaaaggtg agttgcgtcg cgacgaacgc aatctgatgt
     4981 ggaacatcga tccctattta cagacgcagt ggcagctgag cgaaaaactg tcgctggatg
     5041 ctggcgtgcg ctacagctcc gtgtggtttg attccaacga ccattacgtt actccgggta
     5101 acggcgatga cagcggtgat gccagttatc acaaatggct accagccgga tcgttaaaat
     5161 atgcaatgac cgatgcctgg aatatctatc tggcagccgg gcgtggtttt gaaacgccga
     5221 cgattaatga gctgtcttat cgcgctgatg ggcaaagcgg tatgaacttt ggtttaaaac
     5281 catccaccaa cgatacaatt gagatcggca gtaaaacgcg tattggtgat gggctgctta
     5341 gtctcgcatt gttccagacc gacaccgatg atgaaattgt tgtcgatagc agtagcggtg
     5401 ggcgtacgac ttacaaaaat gctggaaaga cccgtcgtca aggcgctgaa ctggcatggg
     5461 atcaacgttt cgcgggagat tttcgcgtaa acgcgtcctg gacctggctt gatgcgacct
     5521 atcgcagcaa tgtttgcaac gaacaggatt gtaacggtaa tcggatgcca gggatcgccc
     5581 gtaatatggg ctttgcgtcg ataggttatg taccggaaga tggttggtat gcaggcatgg
     5641 aagcgcgtta tatgggcgat attatggcag atgatgaaaa tacggccaaa gcgccgtctt
     5701 atactctcgt cggcttattc accgggtata aatacaatta ccacaattta actgtggatt
     5761 tgtttggtcg tgtcgataat ttattcgata aagaatacgt tggttctgtc attgtcaatg
     5821 agtcaaacgg gcgatattac gaacctgcgc ccgggcgaaa ttatggtgtc ggcgtgaata
     5881 tcgcatggcg atttgagtaa ttaccttgat gcccggtatt tgccgggcat ttactttaac
     5941 gattgtattg ctggtataaa atagcaatat tttgttgcat aatttcagca agacaatgtc
     6001 tgcatccctc tttgtctttt gcttttaacg cagcgagtaa cagatgatag ttttctatat
     6061 gctcccgtaa ttccgctgga ttaatcgctt catagagata atgtaaacca ggtcccatcc
     6121 tgacccacag ttgctcaatc atctcacaca ggatgggcat atttgagcga tgataaatcg
     6181 ctaagcgaaa tagcctgttt acattaatga tttgttccat gtcgcccttt tcttgcgcct
     6241 gctgtaattt ctcgagcaat tcctggagtt ccgccaggtc ttgcggggtg aggttttcaa
     6301 cagccagagc aactgccatt aattccagtt cgtagcggat ccgattgatt tcgtctaatt
     6361 gacgtttccc cacttccgga actgtaaatg cttgcgctgg tgcgacagaa agcgcattca
     6421 ccgaaaccag acgtaataat gcttcacgca caggtgtaat actcatacct aattgctccg
     6481 ccagattttt agtaatcagg cgtgcgccag gttttagtgc gccaatacta agctgatgtt
     6541 tcaggtcgtt ctcaacctgc agagtcaaac tgacatgttt cattttttcc gttccaggca
     6601 ttttaattcc ttcgcgtgtt ttctgtccaa ccctgcctgc atggcagaag gaattcacct
     6661 ggtgtattaa agtgaataat cataaaccac tcacctttgt tgttattagc agcagaacaa
     6721 tgatgtggtt agttaattaa agctgaaaat aagctttttg cctggatttt tatggcggct
     6781 tacgccgccg ttgaaacttt aatcatcacc cgccacgcgg atcaccactt tgccgaagtt
     6841 tttacccttc agcaggccga taaacgtctg tggcgcattc tctaaaccgt cagtaatttc
     6901 ttcgcggtag tggattttat cctctttcac ccattgcccc atctccctct gaaactcatg
     6961 gatgcggtga ccataatcct gagcgataat aaaaccttgc aagcgaatac gttttttcag
     7021 cactgtagcc atcaacagag gtaaacgatc cggaccgggt ggtagctctg tagcgttata
     7081 gctgctcact aatccgcaga cgggaatgcg cgcagatgta ttaagtaacg gtagcaccgc
     7141 atcgaatacc ttaccgccca cgttttcata atagatatca ataccttttg ggcacgcttt
     7201 cgccagttgt tcggcaaaat catccgcgtg gtgatcaaga caaacatcga agcctaacac
     7261 ctcggtagca tggcggcatt tttccgcgcc accggctacc cccaccactc tgcaaccttt
     7321 aagtttgccg atttgcccca ccgtcgcccc cactggtcct gtcgccgcag ctaccaccaa
     7381 cgtttcgccc tctttaggct gaccgatatc cagtaggccc atataagcgg taaagcctgg
     7441 catccctagc acacccagcg accacgatgg attttgcgga tgatcgccaa gtttcaccag
     7501 atcatcacca ctggatatgt catagtcttg ccatccactg tagcccagca cccagtcgcc
     7561 agactgataa tcaggatgat tcgactccac gacacggctc accgtaccgc cgaccatcac
     7621 gccgccaata tcaaccggcg gtgaataaga tggctcatcg ctcatacgtc cacgcatata
     7681 cgggtccagg gacaaataaa ctgtgcgcag taacacctgt ccttcacccg gtgtggcgac
     7741 atcatcttct tcaagacgga aattctccgg aaccggtgcg ccatgtggac gcgaggccag
     7801 aacccaacgt cgattacgct gcttttgttg ccccataatg ctctcctgtg cttttggcat
     7861 gaatccatca atagtagcag gctcgcagga ttggttttgt ttgcagccgt caccccccct
     7921 ctgcaacacg caataatttc atcgaggaat ccgttgcttc gggctgtttt cgatagtatt
     7981 gataacatca tcatcattat tttattgagg ccagacatgt ccatccgttt tgcccgcaaa
     8041 gccgactgtg ctgccattgc ggaaatttat aaccacgccg tgttgtatac ggcggctatc
     8101 tggaatgacc aaacggtgga tgcagataac cgcattgcct ggtttgaagc gcggaccata
     8161 gcaggttatc cggtgctggt aagtgaagaa gatggcgtag tgacgggata tgcctcattt
     8221 ggcgactggc gtagtttcga tggttttcgc cataccgtgg aacattcggt ttatgtccat
     8281 cccgatcatc agggcaaagg tctggggcgt aaattgttaa gccgattgat tgatgaagcg
     8341 cgagattgcg ggaagcatgt catggtcgcc gggatcgaat cgcaaaatca ggcctcgctg
     8401 catctgcacc agtcgctggg atttgtcgtc accgcacaaa tgccgcaggt gggcactaaa
     8461 tttgggcgct ggctggatct gacatttatg cagttgcaac tcgacgaacg cactgaaccg
     8521 gacgcgattg gatgaatcag tcgctcaccc ttgccttttt gattgccgcc ggaattggtc
     8581 tggtagtcca gaactcgctg atggtgcgca tcacccagac ctcctctacc attctcatcg
     8641 ccatgttgct gaactcactg gtggggattg tgctctttgt ttctatttta tggtttaaac
     8701 aaggtatggc ggggtttggc gaactggtgt ccagcgtgcg ctggtggaca cttatacctg
     8761 gcttgctggg atcgtttttt gtcttcgcca gtatcagcgg atatcagaat gtgggagcgg
     8821 cgacaactat tgcagtactg gtcgccagcc agctgattgg cggtctggtt ctggatatct
     8881 tccgcagtca tggcgtacca ctacgggcgt tgttcggccc aatttgtggc gcaattttac
     8941 ttgtggttgg cgcctggttg gtagccagac gctcatttta atcacaggat ggtgccgcct
     9001 ttggttaact gttcatggcg cgcttccata tcttctttat gcttgcgacc gtgatgagca
     9061 atagcggtgc gcaaacgctg ctgctgggtg tagcgatctt cccgacttaa ctccgcgtca
     9121 tcgctcaggg cgattaataa ttcgttcata tgggcaatga cgctctcttc aatcgcggca
     9181 tccacgcgcg cgatgacttc atccagatgc gacatacttt ctccttagcc acttgtgaaa
     9241 tttatcgatt catccaaatg atataagtct gcgtggtgcc gtcaagccag tgagtaagat
     9301 ttaaaacagt gaaaacaaca gcgccaccgg gaaactcatc ggccatgttg cccccaccag
     9361 aaaggcgctt aagaaacgga tgcgtttctt atcgtgagaa agaaaccaag tgataagaaa
     9421 tgcgattgct gccataaccg cataaaaaac aagcatgtgt tgatagagag acatgtacgg
     9481 gcgtccagcc agatgaataa aacgcgcgca aatatgcgtc attttttgac gtgcatcaag
     9541 ttttttaacc ggtttgagaa aaaaacatca aacagcaact tgcccttatc catcttcaga
     9601 atgagtatta ttgcggattt tcttaacccg tactatacac ataggggcaa tgataaaagg
     9661 tggcaaaaat gaatgtttcc agtagaactg tagtactgat aaatgtcttt gctgctgttg
     9721 gtttgtttac tcttatctct atgagatttg gctggtttat ttgatgtagt gattttgcac
     9781 gcagcacgta ctgtcagaga aatcagtgct ctgacagtac gcaagatcat ctcatactct
     9841 tacgatctgc aatattatcc ttaatgttta accatgacgt ggcggacgac ggtgtaatcc
     9901 tccagcccat aaagtgacat atccttgccg taaccagaaa gtttctgccc accgtgcggc
     9961 atttcactta ccagcatgaa atgggtattg acccaggtac aaccatattg cagccgtgca
    10021 ctgacgcgat gcgccctgcc cacatctttc gtccataccg aagatgcaag tccgtactgg
    10081 ctgtcattcg cccagtttac cacctgttct tcgttgtcga agagcgtaac actcactact
    10141 ggaccaaata cctctttttg cacgatggca tcgtcctgta atgcgccagc cagcagcgtc
    10201 ggcgcatagt aatagccatt acccttgcgc ttttcaccgc cagtgatcac tttgatgtgc
    10261 cctgtcgctt tcgcctcttc tactgccttg ctgacgcgtt cgagatgcgc cagcgagctt
    10321 aaaggtccaa gctccgtaga ctcgtcatcc ggcgcaccag attttaacgt tgccactgca
    10381 gcaccgagtt tttccaccag cgtatcgtaa atgccttttt gcgcgtagat ccgacaagcc
    10441 acagtacaat cctgtccggc attgtaatag ccaaatgtac gtacaccttc gaccactgct
    10501 tcaatatccg catcatcaaa aacaatcact ggcgctttgc caccaagttc catatgagta
    10561 cgcttaatgg agggcgcggt atggctgatg atgtgctcgc cggtggcgat agagcccgtc
    10621 agcgacacca tccgcacttt gggatgaccg gtcagcggat cgcccaccgt tttgcctctg
    10681 ccaaacagta cgttaatcac gcctgccggg aagatatctt tcgccagctc tgccaacttc
    10741 aacgcggtca gcggggtaat ttctgatggt ttaagcacta cgcagttccc tgccgccagc
    10801 gccggagcca gtttccacgc tgccatcatc agcggataat tccacggtgc gatagaagcc
    10861 acgaccccca acggatcgcg acggatcatc gaagtatgac cttcaagata ttcacctgcc
    10921 gccagaccat tcagacagcg cgccgcaccc gcgaaaaagc gaaaaacatc gacaatcgcc
    10981 gggatttcat cattgaacgc actatgcagc ggtttgccac aattacggga ctccagttcg
    11041 gcaaaaacct gaccattttc ttcgataaca tcagccagtt tcagcagaca ttccgcacgc
    11101 actttcggcg tggtttgccc ccattcggca aatgctgcat ctgccgcgcg cacagcagca
    11161 tcgacctgct ctgcggatgc ctcggcaatc tccagtaaaa cgtcccccgt tgccggatta
    11221 tagacaggct gtttttcccc ttcgccgcta accagttctc cgttaatcag taacttatgt
    11281 tgcatagcat attcctgtat cagtggttat tgaccattgt cgccttcgcg ggttagccac
    11341 caggctccca ggatcggcaa ggttgttacc aacataacca gcagtgccac cacgttagtt
    11401 accggtacat cacgcggtcg cccaagctga ttgagcaacc acaacggtaa cgttcgttca
    11461 tgacctgccg taaaggtcgt aacgatgatt tcatcgaacg acaaggcaaa cgccagcatt
    11521 cctcctgcca gtaacgccga actgagattc ggcaacacta cgtagcggaa ggtttgccag
    11581 ccattggccc caagatccat tgacgcctca accagactcc atgaggtgcg gcgaaaacgg
    11641 gcgatgacat tgttaaacac cacaactaca caaaaagtcg catgaccgac cacgatggtg
    11701 aaaaatcccg gctccagatt gatggtttta aaggcggtta ataacgccag accagtgaca
    11761 atgcccggca gcgcaatggg cagcagcagt aacagcgaaa tggcgttttt gccgaaaaag
    11821 tctcgtcgcc acagcgcggc agctgccagc gtccctaaca ccagcgccat taatgtcgcc
    11881 agcgccgcca ctttaagtga cagtgtcacg gcatcaagaa tatcactacg ctgtgctgcc
    11941 acgctaaacc agcgcagcgt caggccctgc ggtggaaaac taaacgccgc atcttcagtg
    12001 ttaaacgcat aggcggcgat tatcaggatg ggaaaatgta ggaaaacaac gccgccccag
    12061 gccgccagtt tgaggaaaaa cggtgcgcgt tcagagtgca tcaaacgctc ccagacgttt
    12121 cacgaacgcc aggtacagtg cgataagaac aatcggcacc agggtgaatg ccgccgccat
    12181 cggcatattg ccaatcgccc cctgctggga ataaaccata ttgccgataa aatatcctgg
    12241 cgggccaacc agctgcggga cgataaaatc gcccagtgtg agtgagaagg taaagataga
    12301 gccagcggca atacccggga tcgccagcgg caacaccaca tagcgaaagg tttgtcgtgg
    12361 acgtgcgccg agatcagctg acgcctgcaa caatgacggc ggcaaacgct caagcgccgc
    12421 ctgcacgggc aggatcatga acggcagcca gatatagaga aacaccagaa agcgccccag
    12481 cccggaagtt gacagcgtat tgccgcccac cgcaggtaat gtaaggaacg ctgtcagcag
    12541 tggttccagc ccaagatgtt gtaaaaacca ctgagccacg ccatcttttg ccagcaataa
    12601 cgtccaggca taggctttaa caatgtagct cgcccacatc ggcaacatta ccgcaatata
    12661 aaaaaacgct ttcattttcc cgctggtata gcgcgccata taccacgcca ttggaaaagc
    12721 cagaatggcg ctggcgatag tgactgccac agccatagtc agcgtgcgga gaatgatgtc
    12781 gtaattcgcc ggattaaaca gcgcacggat gttcgccagc gtcagttccg gcgttaccga
    12841 catggtgaaa tcgtcaaagg tataaaagcc ctgccataac agcgttagca gcgagccgaa
    12901 atagacaatg ccaaaccaca ttagcgggcc aagcagcagc aaaaacagcc ccagccccgg
    12961 attccgccag aagaaaccgg acaccttacc cagacctgga cgtgaaggtg attgcaatac
    13021 gttcatcgcc attcacctct cctcaaccag cggcaccatc acatcacgcg accaggaaac
    13081 catcacctgt tgtccgggcg tgagcgtggc aggcagttct tcgcctgtca tattggcctg
    13141 actcaccagc agtttttcac cgccgttcaa tttcagttca aaacgagttg ccgcgccctg
    13201 atattgcacc gcctggatcg tgccattggc ctgcagttca ccaggggtgt tgaggcggat
    13261 atgttccggc cgcagggcga agcttcccgt catgccacaa agtttctctg ccatcagtcc
    13321 atcaaaaaca ttcgatgtac caacgaaccc ggcaacaaac ggcgtgcgcg ggcgcatata
    13381 gagatcgcgc ggggaatcga cctgctcaat gcgtccatta ttgaaaaccg ccacacgatc
    13441 ggacatcgat aacgcttcgc cctgatcgtg ggtaacgcag ataaaagtga taccgagaga
    13501 ctgttgcagt tttttcagct caagctgcat ctgctcacgc aatttgagat ccagtgcgcc
    13561 gagcggttca tccaacagca atacgcgcgg ttcattcacc aatgctctgg cgatagcaac
    13621 ccgctggcgc tgaccaccag aaagttgtga cggtttacgt tgctgtacaa accccaacgc
    13681 cactttctcc agcgcctctt gcgccattgc gtgccgctgc tttttattca cgcctttgac
    13741 catcagccca taggcgacat tgtcaagaat cgacatatgc ggaaatagcg cgtagtcctg
    13801 aaagacagta ttcacgtccc gctcccacgg tggcagattg ctggcgggtt taccaaagat
    13861 agagatagcc ccgccggaaa gctgttcgaa gccagcaatc aggcgcaggc aggtggtttt
    13921 gccggagccg gacggcccca gcatagagaa gaactcacca tcttttatcg caatactgac
    13981 gccatctact gcgcgcacgt caccgtacaa ccgcgagacg ttgtcaaact ccactgcgta
    14041 cgtcataaag caccccagcg aagttagcga ccgcccataa tggcaatgta atcctgcgtc
    14101 cagcgactgt agggaacaaa cttgccccct tctgctatag gcgttttcca gaaggctatt
    14161 ttatcgaaat agttaaaacc gtttgtttca caacctttct cgcctaataa cgaactggct
    14221 ttacaccctt ccggcacgac tggtaacgag ccaaaccagg ccgccacatc gccctgcact
    14281 tttggcgtta atgaccagtt catccatttg taggcgcaaa ccggatgttt cgcttcgcta
    14341 tgtagcatgg tggtgtcagc ccaaccggta acgccctcct tcgggaaaac ggtagcgaca
    14401 ggctgacctt cggctttcag ggcgttggtc tgatagggcc aggcactgga agcgaccaca
    14461 ccctcgtttt tgaaatcgct catttgcacg gtagtgtcat gccagtagcg atggatcaaa
    14521 ctatgttgat cacgcagcac tttcagcacc gcctggtact gttcttcggt gagttgatac
    14581 ggatcgctga tgcccaactg cggctgagtg gctttaacga acaacgcagc gtccgcaatg
    14641 tagatagggc catcataagc ctgaacgcgg cctttattgc tcttgccgtc ctgcagattt
    14701 tgctcaacaa aaaccacttg ccagctatcc ggcggcgtcg ggaaggtttt agtgttgtac
    14761 atcagcaggt tcggccccca ttggtaaggt gtgccgtaaa ctttgccgcc aacgttaaac
    14821 cagtcgcctt taaccacgcg cggatcaagc gttttccagt tgggaatcaa ggcggtatta
    14881 atcggctgca cgcgtttacc cataatcaaa cgcagcgagg catcgccgga tgccgtaacc
    14941 agatcgtaac cccctttggt catcagactg accatctcat cggaagtcgc ggcggtttta
    15001 acattcaccg cgcagcctgt ctctttttcg aattgcgtta cccagtcgta ttgtttatca
    15061 gtttgtccgc gttcgatata tcccggccag gcgataatat ccagtcgccc ttccggttta
    15121 tctaaattgg taggcggttc ggcggcgtga gcggtcatta ttgtcatgct gagcgcacac
    15181 aggctgctgc gggcaaatgt cttgctcata aggtcttact cctgtcgtaa ttaattgttc
    15241 agccgccgtg ccgtaaaaat atctccttgt ttaaccatag acggcggctt gcaggcacac
    15301 cttcgcccct gagaaaattt aatcaggttg tgagctaccg cgcattatgc cgtctgtaag
    15361 gttatcttct ggagtataga caaggagtta ggaaatatga ctgctattcg aaattcatat
    15421 tacccaatct aagaggaatt aaattaaacg ctattacagc cgttcttgaa taagtttgcc
    15481 taattgtttt accgcctgtt cttcacgttc tccccacagc cacgcggtat taaaacgaaa
    15541 aaaacgtgac cagttttcac cggtagaaaa cattttacct ggcgcaatac tgatatgatg
    15601 cgtcagtgcc gccaggctca attcgccggc atctaacggc tcggggagct ccagccagag
    15661 aaaataaccg ctgtcattat gatgaatttt cacttccgca ggcagataac gcagcagtgc
    15721 ctgccaggca cgttgtttac gttccgcaag ctggcgacgc aggcgacgaa gatgggcgtc
    15781 gtatcggcgc gtggaaaggt aatccaccag cgcaagctgc atcggtgagc tggtggaaag
    15841 cgtactcatc agctgcaagc gttgaatttt acgtgcatgt tttccggcgg cgacccaacc
    15901 aatacgaaaa ccaggcacca gacatttcga aaacgaagag caatgcaaaa cgccatcgtg
    15961 gcgatcccac gctttcgcag gcagcggttt ttcccgtcca aaataaagtt cgctgtaaac
    16021 gtcatcttca atcagcgtta cgttgtactg attgagcaac gccaccagtt gtgctttttt
    16081 ttgcggcgtt aaggtaaatc cgagtggatt ctggctatta gtcatcagcc agcacgcttt
    16141 caccggatac tcctgcaacg ccagttccag cgcctgaaga tcgatccctt ctttaacatc
    16201 cgtcgccacc gataacgcct tcagccgtag ccgctccagc gcctgcaacg caccgtagaa
    16261 acaaggattc tctactatca cccaatcgcc cggttcagtt accgcttgca aactgaggtt
    16321 taatgcctct aacgccccgg cagtgatgac aatttcatca ggagaaatgg tgatcccctg
    16381 taaggcatag cgacaggcaa tagcctgacg cagttctgcg tttcctggcg gcaagttttc
    16441 aatcacgctc atcgccgtgg cggttttgct tacctgcgcc agcgagcggt ttagttgttg
    16501 gagggggaaa agtcgcgggt cgggaaaggc cgaggcaaac ggaacgaccg acggatcgcg
    16561 actggcctgc aacatatcaa aaatataagt gttgatatcg actgcttcat ctcgagtgac
    16621 tggaatgact ggcactttcg gcatttttat tgcctgtggc gcaacgtaat aacccgactg
    16681 cggtcgtgcg ataatatatc cctgactttc gagcaactga taggcatggc tgacagtcat
    16741 aaagctcatg ccagaaagcg ccacctggtc acgcaacgaa ggtaaacgat cgccgggttg
    16801 ccagataccc gacgcaatct gctcgcgtaa ttgttctgca agctgctggt attttttcat
    16861 tatcgaatcg taattatgtg cagatgattc ggcagtctat atcagttgtt aaaaatgaca
    16921 acttttcgtt aactgtaatt aaaccatcac cagcgataac tctttgccaa gcgcctttgc
    16981 cgcgagctgg actgcgtcga tttttgtcgc atgatgcaag ttaaataggc gagtaatctc
    17041 ttgtttaggt ttgccaattc gcctggctaa ctcttgctga gtgatttctg actgtaaaaa
    17101 agcatttaac agcaatacct tagaggcgac gctcaaaggt acttcaataa agtgatcatg
    17161 actatttaat ggcgaaggta aagggataag ctcgttatct tcaaaataaa aatcaaatgc
    17221 ggtcagtaaa gcatcttttg ccgcttccat cgcttcagcg acagtttcgc cctgcgtcaa
    17281 cgcttcaggg atatccacaa aagaaaccat ataaccgcct tccggcgcgg gtgtaagagt
    17341 gacgggataa cgcataattt cgactgtctc tcgcatgtca ttaacctttt taattggcga
    17401 ttaactcaaa ccgagttgtt tcaggattgc tttacgcaat ggttctttaa tctcatcgca
    17461 ggggtgacgc ggcatgacac tgcgcctccc atgaaacctg agtttcaaat ggttgctgcc
    17521 attcgctaca tcgacgccct gagattcgag ccaacgtctg aactcgcttt gtttcacatc
    17581 gcctccctgc ggctgagtat atcaacaaag taaacattag tgtttacctc cgtgtgtaat
    17641 ttgctcgaaa atagcactaa atcgccgcaa cggcagaatg caatgcaaaa tcaggaagga
    17701 agattccaga atttctttgc gctaatttgc aaaactgaat aataacctca agaaacgatc
    17761 ggcatcatag cggcaaaagg taagaaaaag gtggcaggat gtttacgaag gcgttatcgg
    17821 ttgtcttatt aacgtgtgcg ctgttctcag gacaactcat ggcagggcac aaaggacatg
    17881 aatttgtgtg ggtaaagaat gtggatcatc agctgcgtca tgaagcggac agcgatgaat
    17941 tgcgtgctgt ggcggaagag tcggcggaag gtttgcgcga gcatttttac tggcaaaaat
    18001 cgcgcaaacc agaagcggga caacgttgag tcgtttgatg ccggatgcgg cgtaacgcct
    18061 tacccggcag gcaatatcat gcagattcaa taagttgtga gtaaccagaa aactggcgtt
    18121 ttaccctttt cgcttcggca atgttttcag cagctcatcc ggacttacgg atgccactac
    18181 gcttcccggt aacggcattt tcagtatgtg atttttgatt ttgccaatga cgtgcatctc
    18241 gcatgggcgg caatcaaact ttagcgttaa tacttcatcg ccgttaacca gttgcattgg
    18301 cgtcgccttc cagcttttga tattgccttt cgcctgtttc gggcacagat taaaggcaaa
    18361 gcgcagacaa tgcttggtga tcatcaccgg gacttcgccc ttctcttcat gtgcctcata
    18421 cgccgcgtca atcagctgca caccatagcg atgataaaat tcacgcgctt tctggttgta
    18481 aacgttcgcg aggaaactca gatgtgtttg cggataaacc ggcgcaggat cagcaaccgg
    18541 tttacggctg ccgcgctggt aactggcaag acgcgcagca tccagcatgt cggcagcttc
    18601 acggcggaac tggtttaaca gactgtttgg tacaaacagc gcccccggca aattaatttg
    18661 cacatcgcgg gcgtaataga gggtttgccc cagttttgcc agaccatcct tcagattgtt
    18721 cattgctttt tctgcgttat tggcttcgtc gaactgccca tccagcgtat gcgttatgct
    18781 gaccccctct tcactggtga gagtcagaat caattgttcc tgccaaccgc ccagttcaat
    18841 gtctaccgcc acccgacgtt cgctagaggt ttttgtcagt gcctgctgcc agttatgatc
    18901 aagattacgg tttagtgggt gatgcgggcg aattttgtgc aaatctgctg gcatttcatt
    18961 gggccagacg cggtactgat tttctccggt tttctcgacc gtattggcac gaaaaccgac
    19021 gacttcacgt ttaatcaaca cgttcaggcc atcgccattt gccagtggct cggtaacggc
    19081 aacatcgaga tgatctttcg ccactttcac tacttcacct accggcaggc cgataaattt
    19141 cggcgaatcg aacgcgccaa tatcgccttt acgggcattc acaaaataat ctgtgctacc
    19201 acggtggaaa gtcttttccg tcgatggaac aaagaaatgt tcagtacgac ctgatgaagc
    19261 gcgcgccaga tcgccacgtt cttcaataat ggcatcaagc atctggcgat aatgggcggt
    19321 gatattcttc acgtagctca tatctttgta acgcccttca atcttgaagg aacgtacgcc
    19381 agcatcaatc agcgcgccga ggttggcagt ctgatcgtta tctttcatcg acagcagatg
    19441 tttttcatag gaaaccaccc gcccctgatc gtctttcaat gtgtatggca aacggcacgc
    19501 ttgcgagcaa tcgccacggt tggcgctacg ccctgtttgc gcatgagaaa tgtagcactg
    19561 acccgaatag gccacgcaca gtgcgccatg aataaagaat tcaatggtcg cgtccgtggc
    19621 ctggtgaatc gcgcggatct gatcaagatt cagctctcgc gccagcacaa tctgcgtgaa
    19681 gccaacatca gagaggaact tcgctttttc tactgtacga atgtcgcact gcgtactggc
    19741 gtgcagttca atcggcggaa tatcaagttc cagaatcccc atatcctgaa caatcagcgc
    19801 atcgacaccg gtctggtaga ggtcagtaat cagccgttgc gcgggttcca gctcatcatc
    19861 atgcaaaatg gtgttaagcg tgacgaaaat ttttgcacca taacgatggg caaacggcac
    19921 cagctcggca atatctttca ggctattact ggcattatga cgggcaccaa aaccagggcc
    19981 gccgatataa acagcatcgg caccgtgcaa aatagcttcg cgggcaatgg cggcatcgcg
    20041 tgccgggctt aacagttcaa gtcgatgaga agatacggtc acattcttat ccagtagctg
    20101 aaaaatggcg gctattttag ccacaagcgc gacgttgcga aatagttttc ccgcttaact
    20161 gcgcgggtaa tggatgaggg aatgaaaatg tacggtttgc tccccaccat tacgatagat
    20221 atgcgtgacg tctgcagcaa atcgaacgcc ttccccacaa ttaagggtct gccattcacc
    20281 atcaacgcac agatcaagtt gtccatcaat gacgacgaca tgttcgatta cccccttctc
    20341 atggggcgtc gattcgctta ttgcaccggg tgccatctga atcgagaagt gttcaaagca
    20401 gagttgtgga tcataaggaa acagcgaaat gatcaccatc gcctgctgtt gtggatcata
    20461 aactgaaggc gtagcggact gtggcggaga aataaatgtc gaaaatggca cgttcaaccc
    20521 agtggcaatt ttccataatg tcgcgaccgt cgggctggac tcattacgct caatctgtcc
    20581 taacattgct ttagaaacgc ccgtcgcttc tgccagtcgc gaaagactcc agccgcgctg
    20641 ctgacgtagt tgtttaagtg tggtggataa aaagcgagcg agattttcca tgcgactcct
    20701 ccggcaaaac ggaagtttat cacttgtgcg ttataacgga caaatgctac ggtgcctgta
    20761 cgctataacg cacgaggtga ctatgcgtct gttttctgtt cctccaccca cgctactggc
    20821 ggggtttctg gcggtattaa ttggctacgc cagttcagcg gcaataatct ggcaagcagc
    20881 gattgtcgcc ggagccacca ctgcacaaat ctctggctgg atgacggcgc tggggctggc
    20941 aatgggcgtc agtacgctga ctctgacatt atggtatcgc gtacctgttc tcaccgcatg
    21001 gtcaacgcct ggcgcggctt tgttggtcac cggattgcag ggactaacac ttaacgaagc
    21061 catcggcgtt tttattgtca ccaacgcgtt aatagtcctc tgcggcataa cgggactctt
    21121 tgctcgtctg atgcgcatta ttccgcactc gcttgcggca gcaatgcttg ccgggatttt
    21181 attacgcttt ggtttacagg cgtttgccag tctggacggt caatttacgt tgtgtggaag
    21241 tatgttgctg gtatggctgg caaccaaggc cgttgcgccg cgctatgcgg taattgccgc
    21301 gatgattatt gggatcgtga tcgttatcgc gcaaggtgac gttgtcacaa ctgatgttgt
    21361 ctttaaaccc gttctcccca cttatattac ccctgatttt tcgtttgctc acagcctgag
    21421 cgttgcactc cccctttttc tggtgacgat ggcatcgcaa aacgcaccgg gtatcgcagc
    21481 aatgaaagca gccgggtatt cggctcctgt ttcgccgttg attgtgttta ctggattgct
    21541 ggcactggtt ttttcccctt tcggcgttta ttccgtcggt attgcggcaa tcaccgcggc
    21601 tatttgccaa agcccggaag cgcatccgga taaagatcaa cgttggctgg cagctgccgt
    21661 tgcaggcatt ttctatttgc tcgcaggtct gtttggtagt gccattaccg ggatgatggc
    21721 tgccctgccc gtaagttgga tccagatgct ggcaggtctg gcgctgttga gtaccattgg
    21781 cggcagtttg tatcaggcgc tgcataatga gcgtgagcgc gacgcggcgg tggtggcatt
    21841 tctggtaacg gcaagtggat tgacgctggt cgggattggt tctgcgtttt ggggattaat
    21901 tgccggaggc gtttgttacg tggtgttgaa tttaatcgct gacagaaacc gataataaaa
    21961 agcgccatct aacgatggcg cgtctggaac tactttttgt taacgtcaaa catggtggca
    22021 tcggtcgcca tgtcatcaat aacttgttta atattttcga aagccattgg cgtactttcg
    22081 ttattcaggt ctttaccttc gccctgacga acgactttga taaccggttt attagtcgct
    22141 gcatcaatca gctcaccttc aaaatacaag cgagtatcca tggtacggtg gcctgtagcc
    22201 atttgcgtcc ccgcaaccac taatgcaaca ggaaccactt catagaattg cagcccttct
    22261 ttgctggtat ctacaccggt aatggcccca cggaaaatca gactacgcgg ccccgcggtg
    22321 gtaaccagtg gtttacgctg cgctatcgct tctttcattt cggtgttggt atagttcaaa
    22381 attttatcca gaactttctg ccctacctgg gtcgaaggtt tcggtaccgg ataataagtg
    22441 attgggttcc agacgatgct gtcatatttg ctttgatcaa aactcgggtc tacccaacgt
    22501 aaaaccggtt tacctgtagc cgaggttgtt tcttttaaat cagagtaatt gtttaaaaaa
    22561 ccagaatatt tatctggctg ggtgatttta gaggcacaac cagacagagc caacaagccg
    22621 cataaagctg caactttcgc aaatgatgtg gtacgcatga atagtttcct gagatgatta
    22681 tattactttt taagttatag caaaaagcgc cgcttcgtgt tgtggcaaaa aaagtgttgg
    22741 tgaaaataaa tttttttagc cccttttcag acgacatagt caatgccgca aaaggggcta
    22801 agtcaatcat cagtagttaa atttcagtaa gttaagctgc taaaaaactt cgactactgt
    22861 gattaatttc acatcccttc gataaaaaat atgcttaccg aaaagaagga cagtaaacca
    22921 tctcagcgtg gggaattttc acgcgctttg ttcttaccgg gtcatttttt acgtgccagc
    22981 atcgtggcga aacgcagttt aatacgatta ccgttggcgt cggtgcggtg cagctcgccg
    23041 acgtcttcat tgtatttcac catctcccag ccttcgtaat atcgacgtaa ttctccctct
    23101 ttgaaggcaa acgggaagcc gacggtacat ggataatcag cggtatccat cgccgccaca
    23161 atcaggttgt aaccgccagg tttagtgcaa cgttgcatat tggcaatcag ccctgggatg
    23221 gttttagcct caaggaacat cagcaccaca gtcgaaagaa taaaatcgta ctctccatca
    23281 aatgtgaggt tattcagatc aacgacccgg gtgtgtaaat tatccagatt ttcaatggat
    23341 ttaatgcgct cgacgttggc gatactcatg gcatttttat cccatgcgtc aacatcataa
    23401 ccattggctg ccaggtaaag actgttacga ccattgccac agcccagatc cagcgtttta
    23461 cccggtttaa ccactttcac cgcttccagt acttcagagt gtgtgcgggt taattcatat
    23521 ttatcagtaa aataattttc gtcacgaatg atcattcttt gtcctctgct ttcattaaaa
    23581 cggcgcgctc ggttctgact aacaattttc cctgcatcag aagcgccaaa gtacggatga
    23641 gcagtattgc aataataaaa ttggtaaaga taaacagcgg caccgccagc gtatggaaaa
    23701 atccattatc gctgccactc cccagatgca aaccggtggt tgccagtgca gatacgccga
    23761 acgagaaact ccagaatgaa gcattaaatg gctgggagag ataccatggc atcagacgta
    23821 gcataaacag cagttgcagc agtccataac cgaaaagcat tttcgccagc gtgtcaccct
    23881 cgccgccgtt gacgctcagc caggcactac aggccaccag cgcaggagcg agctgaatgc
    23941 cgagtgatgt ccgcagtgcc gtgggtaatt ctcccgaact acgcagacgc tgcaagatca
    24001 ccggttcaag gcttagccat gagaaaacgc ctgcgcctaa aaacaccaga ccggcgtcgg
    24061 tgtagcccaa cgcaccacag gccattgcgc tgataaagtt gttggcaact gtcggcagat
    24121 acagtccagg cgtagtagct tcttcagggt gagatccgcg ccataatccc gcagtttgcc
    24181 aggcggcata agccaactga accacgacac caaaactgaa caggcacacc gccagtgggc
    24241 gaaaccacgg aacaaaacca atcgccacca gcatcgtcgt tgcaggaaac aaactcacaa
    24301 aactgctcag cactggatgg cgaacttccg ccagcacgct atgcggaaag cgtatgagtc
    24361 gggtaataaa tgcgctagtc aataatcccc agatgatcat cgccagaatc accagcccat
    24421 cccctaacca gtggctgacc tgccaaacct ggctggcata gcgccaggca aatcccatcc
    24481 ctatcgtccc caacacaata ccaaagtagc ctgccggcaa attgagcact ttatcgctct
    24541 gcatcttctt actcattttg tttaatttat aaagtatatt tgaaatgcat tttatggaat
    24601 tttctcgagc atagccagag ccgcagaatt tgctacggtt atagtaagtc acctgtggga
    24661 aaacgctatg cgcaaatatc gtctaagtga agaacagcga gcctttagtt atcaggaaga
    24721 tggcactaaa aaaaatgtgt tattacggca gattatcgcc attagcgatt ttaacgatgt
    24781 gatagcagga accgctggcg gctggatcga tcgcgaaacg gtgttagcac aggaaggaaa
    24841 ttgctggatt tacgaccaga atgctattgc gttcggcgga acggtgattt ccggtaatac
    24901 gcgcatcacc ggcaccagcg tactgtgggg agaggtttac gcaacggata atgtctggat
    24961 cgataatagt gaaatcaacc agggtgcata tataagtgac agtgtcacca ttcatgactc
    25021 actggtttgc gggcaatgta gaatatttgg tcacgcctta attgatcaac attctatgat
    25081 cgtcgccgca caaggcttaa cgcccgacca tcaactcctg ctccaaattt atgatcgcgc
    25141 aagggttagc gcctcgcgta ttgtccacca ggcacaaatt tatggcgatg ccgtcatccg
    25201 gtatgctttt attgaacatc gcgccgaagt ttttgatttt gccagtattg aaggtaatga
    25261 agagaacaac gtctggctat gcgattgcgc taaagtctac ggtcacgcgc aggtgaaagc
    25321 tggcatagaa gaagatgcca ttccaacgat tcattacagt tcgcaggtgg cggaatatgc
    25381 cattgtggaa ggtaattgtg tgttaaaaca tcatgtcttg attggcggaa atgccgtggt
    25441 acgtggcgga ccgattctgc tcgacgagca cgttgtaata caaggtgaga gccgtattac
    25501 aggtgcggtg ataattgaaa accatgtgga gttaaccgac cacgccgtcg ttgaagcctt
    25561 tgatggcgac acggttcatg tccgtggtcc aaaagtcatt aacggcgagg agcgcattac
    25621 gcgaacgccg ctggcagggt tattatgaat cgataatacg cgcgtataag ttcacatcat
    25681 cataggcatc attcaggaac tcagcctgtt tcaggcaacc ttcaaggata aaaccattgc
    25741 gcaaagcgac ctggttgctt tgcggattgt ccacccgaca tttgatcacg aagcgtctaa
    25801 gttcaccaga ctgagcgtaa tgatgaatca gtgcctgcag cgcctgagaa atgatcccct
    25861 gcccctgatg agattcgtcc agtcagtagc cgatttcagc ggttttattc agtggttcaa
    25921 tacgattaaa cgagataacg ccgataagtt catcttcttt gaaaatcatg aacattttgg
    25981 catagccgcg ttgatgcaac atcacattac cctgcaccgt ttttcgcgtg tcctcttcaa
    26041 tctgaacgaa ctgcggccag tttagcgact gctgtaacca ggttttattt ttacagatta
    26101 actgataaag aggagtgacg tgattttctg caacagcatg taattcaagt gattcgctta
    26161 cttttatcgt ttcagtcatc ttatctccag tcaatccatg cagttgttaa aaaagccgcc
    26221 tggctggcgg cttaggaaag tttacacctc tttgacgctc tcatgctgca ggattttgag
    26281 catctcatct tttaactgta gcttttgttt tttcatgcgg accacttccg cattgtaccc
    26341 tcgatcgtcg gaaccttcct ttctggcaat ttcatgatca agtttattgt gtttatcgaa
    26401 caaggacata aagcgaggat tttcgttttt cagacgggat attaaatctc gatattctgg
    26461 aaacatacgc actccctgtt agtggctgac aaggtgtatc tgaaatacac accatcagat
    26521 tactcattta acttagggaa aaatgcgagc gggatcgtgt ttaaccagac ggcacctccc
    26581 gaagaaggtg ccgaagaaaa aacgattaac tcatcacgcg gtcgtcaaca tactgacgtt
    26641 tatccggcgc tggcgggaaa tactgataca gccatgtttc actgatagcg tcgccctgac
    26701 aacgcagata catccgcata tcgaccggat cagtggagtc cgaagtcgga taccagtcaa
    26761 actgaatacg ataaccatca atgggttcaa tatagagaat ttcgatttgc ttcgcttccc
    26821 cactggaaag cgtaatcacc ggctcaatgc cttttggtgc ggcagctttc agatcaccac
    26881 caacgaaatc gacggcaaaa cgacgcgccc atttttcggg atagtgttca cctggtgccc
    26941 aaccttccgg gaaaccgccc atgccggtac gcgtcgccat aacgcgcgct aatgggcaat
    27001 gaacaggcgg ttgcgcactc cagtacagac gatactggaa tgcaaactca tcacccgcct
    27061 ttacagcttt ttctggctgc cagaagcaga caatgttatc cagcgtttcg cccgttgttg
    27121 ggatttccat caggccgatg gtgcccttac cccacttgtt acgcggttcc acccacagac
    27181 ttgggcgttt gttataccag cccataatgt cctgataatg ggagaaatca cgatccagtt
    27241 gcaataaacc aaaccctttc gggttgttgt cggtgtaagc attgaactgc aatttttgcg
    27301 gattattcag cggacggcaa atccactcgc cgttgccccg ccacatggac agacgatcag
    27361 agtcatgaat ttgcggatga atggtgtcgc acatccgacg ttcattagta ccgcagctga
    27421 acatactggt catcggcgca atgcccagct gtttaatgtc tttgcgcgca tacaggtgat
    27481 tttccacatc cataatcacc tgacttttct cacaatggat agtgaactta taggcaccag
    27541 taatgctggc gctatcgagc aacgcataaa cggtaaaggt agttgccccc ggttttaccg
    27601 tatcaaacca gaaggcggta aagtcgggga actcttcttt actgtcggtg taagtgtcaa
    27661 tcgccaggcc gcgggccgac aaaccgtatt gatatgtgtc atcaacggcg cggaaataac
    27721 tcgctccgag gaatgatact acatcacggc gcgccagttc gggggcttta aacacgcgaa
    27781 aaccggcaaa gccgagatcg ctttgccctt ctaattgttt ggtatcaaca cctgcatcgt
    27841 tgtatttgaa caactccggg cgaaagtgaa tttcacgcgc cagatgtgtt gctggatcta
    27901 cagaaaacat acgaacgcgg cgacggaatc ccattcccat atggaagaac tgagcgtcca
    27961 gttgacggtt ctcaacgtta tgccagagcg atttttcggc gtcgtattga atactgttat
    28021 aagcctgcgg cgtcattgtc gccagcgtgt caggtaacgg acgaggcgca ccacgccacg
    28081 ctgtttgcgc taagtcgtgc gccattgact gtagaatgga gaagtcaaaa cgctgggttt
    28141 gcccgtcggc aatatcagaa tctgccgcga atgccgcctg agaaaaaaga gaagcaatgc
    28201 cgctggtacc gcacacggcg gccatagcca ttgaaccttt aataaatcgt ctacgatcca
    28261 tacctgaaag tgagtccttc tggtgttgtg aatggtcccg cgctggtcgt tttcagcggc
    28321 aagaaagtgc ttatt
//
LOCUS       DAZFDC010000056        28161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFDC010000056 DAZFDC010000000
VERSION     DAZFDC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.056"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1391)
                     /gene="ccp"
                     /locus_tag="ACHJ48_002492"
     CDS             complement(<1..1391)
                     /gene="ccp"
                     /locus_tag="ACHJ48_002492"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO4708038.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLDVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQD"
     gene            1796..3445
                     /locus_tag="ACHJ48_002493"
     CDS             1796..3445
                     /locus_tag="ACHJ48_002493"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO4708039.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3496..4098)
                     /locus_tag="ACHJ48_002494"
     CDS             complement(3496..4098)
                     /locus_tag="ACHJ48_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO4708040.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGIMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4618..5517
                     /locus_tag="ACHJ48_002495"
     CDS             4618..5517
                     /locus_tag="ACHJ48_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4708041.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQTPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5566..6579
                     /gene="yhjD"
                     /locus_tag="ACHJ48_002496"
     CDS             5566..6579
                     /gene="yhjD"
                     /locus_tag="ACHJ48_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO4708042.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            6991..8313
                     /locus_tag="ACHJ48_002497"
     CDS             6991..8313
                     /locus_tag="ACHJ48_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4708043.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLSAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8495..10555)
                     /locus_tag="ACHJ48_002498"
     CDS             complement(8495..10555)
                     /locus_tag="ACHJ48_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO4708044.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10625..11392)
                     /gene="pdeH"
                     /locus_tag="ACHJ48_002499"
     CDS             complement(10625..11392)
                     /gene="pdeH"
                     /locus_tag="ACHJ48_002499"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO4708045.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTAVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11624..12553
                     /gene="kdgK"
                     /locus_tag="ACHJ48_002500"
     CDS             11624..12553
                     /gene="kdgK"
                     /locus_tag="ACHJ48_002500"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO4708046.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESDQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12649..14145)
                     /locus_tag="ACHJ48_002501"
     CDS             complement(12649..14145)
                     /locus_tag="ACHJ48_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO4708047.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14366..15652)
                     /gene="dctA"
                     /locus_tag="ACHJ48_002502"
     CDS             complement(14366..15652)
                     /gene="dctA"
                     /locus_tag="ACHJ48_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO4708048.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDALNNRAPDGKTHELSS"
     gene            complement(15835..17823)
                     /gene="hmsP"
                     /locus_tag="ACHJ48_002503"
     CDS             complement(15835..17823)
                     /gene="hmsP"
                     /locus_tag="ACHJ48_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO4708049.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVHEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSISVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSVLQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17905..21378)
                     /gene="bcsC"
                     /locus_tag="ACHJ48_002504"
     CDS             complement(17905..21378)
                     /gene="bcsC"
                     /locus_tag="ACHJ48_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO4708050.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADEPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWALQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLRDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDFPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21360..22466)
                     /gene="bcsZ"
                     /locus_tag="ACHJ48_002505"
     CDS             complement(21360..22466)
                     /gene="bcsZ"
                     /locus_tag="ACHJ48_002505"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO4708051.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22473..24830)
                     /gene="bcsB"
                     /locus_tag="ACHJ48_002506"
     CDS             complement(22473..24830)
                     /gene="bcsB"
                     /locus_tag="ACHJ48_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO4708052.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24841..27459)
                     /gene="bcsA"
                     /locus_tag="ACHJ48_002507"
     CDS             complement(24841..27459)
                     /gene="bcsA"
                     /locus_tag="ACHJ48_002507"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO4708053.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGGALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTVGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27456..28161)
                     /gene="bcsQ"
                     /locus_tag="ACHJ48_002508"
                     /pseudo
     CDS             complement(27456..28161)
                     /gene="bcsQ"
                     /locus_tag="ACHJ48_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
BASE COUNT         6628 a         7813 c         7106 g         6614 t
ORIGIN      
        1 ttatcctgca tatacggcgt gtaaaccccg ttcagactgt gcaggaaagc tacgatatca
       61 tccacatcct cctgcggcag ctctttgcct acctgatagc gcaacatcag tttcaccgcc
      121 ccgtccagcg tcggcacgtc accgcgatgg aagtacggtg ccgttaaagc aacgttacgt
      181 aaaccgggta ctttctgacg caatttatca cgctcttctt tagtcacatt catacgacca
      241 atatccgccg ccgtaatttc cccaaagtta aagtcttttt tcagccccaa cggttcgaag
      301 gaacgtccgc cgagaataat tccaccatga caagttgcac atttattatc tttaaataat
      361 tgatagcctt ttttctgttg cgccgtcagc gcattttcat ccccacgcaa ccatttatca
      421 aatggggaat ccggcgtaat taatgttttc tcaaattcag caatggcatc agtaatattt
      481 tcgccactga aaccttgcgg atagacgtcg aggaactgcg ctttaagctg cggatctttt
      541 tccagcttag caataatttc gtcccaggat tttgacgcca tttcaatcgg gttcaacggc
      601 ggtccaccag cctgatcctg caatgttgcc gcacgaccat cccagaactg ctcaacgtta
      661 aatactgagt taaataccgt cggcgcgtta atcggcccaa ctgcgccacc aacaccaatc
      721 gatgtttttc tgccatcgac gccccccgca ttcaacgcat ggcaatgagc gcatgaaatg
      781 gtgctatcag ccgataaacg gggatcgtga tacagcgcaa agcccaacgc cactttttgc
      841 gcatcggtag gcagtttttg cgggatgggc tgcaccggtt cattgcgatg ctccggagca
      901 gtatcattgc tggcgtaata ttccgcgcgc tgttttgcaa tccaggccag tatttccgcc
      961 cgctcttcat cactcacctt acccgcccag tgtagcgcgg tataacgcgt tggcggcata
     1021 gtttcatact gcatcaccca ttcaatctta ttcagatcgc tttgcgaaac gggtttatca
     1081 gccagcagag cagcacgcac ggcctcgagg ttaaaagatt tatatccaag cttaatgtcg
     1141 taatccatca actgtttcgc gccaggaata taataatagg cgggtaattc tgccgaaggc
     1201 gtgtggcaat agtcgcatcc tttttcgcgg agaaagccta aaaccttatt attttcactg
     1261 acagcagatg cctgaacatc ggcctgttta ctgcgtttat tatcgtggta ccacacataa
     1321 ccagataacc ctaaatagca aatcgcgacg ccagccaggc cgatcgcggt aatacgtgag
     1381 accattttca ttattcttcc ctcacggtgg tgagttattg tgacccagaa aattttccag
     1441 aatgctgcaa acgtctctgc gagacaaagc catcataaaa aacagggaga tacgcttttt
     1501 gataggaatc aatcaattac atgaatataa actatcaata agatagcctg tgcctattac
     1561 ccagatcagg aatggcatat catgattgcg gcaagagcag gattgaatgt tctgaaaatg
     1621 aaaaacaaca cgaaatctgt atttttataa attccattga tattagtgcg ttatattttt
     1681 tgacaggtta ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga
     1741 ctacccttaa agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct
     1801 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaggtcg atctctgcta
     1861 tgaactggac ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
     1921 gatgattgaa gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact
     1981 gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc
     2041 acccaaaatg gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga
     2101 ttttgacttg cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga
     2161 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
     2221 aacccgcgaa ccacaggatc acattccgtg gtcttctctg ctggcgctgc cgcagtcata
     2281 tattgtcccg ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct
     2341 ggggctggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
     2401 gatgatcgaa aactacggtc acatccccaa cggcaaccgc acctattatt tgagccgctc
     2461 gcaaccaccg gtttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc
     2521 gcgccgctat ctcgaccacc tgaaaatgga atatgccttc tggatggacg gtgcagaatc
     2581 gttgatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     2641 ccgttactgg gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac
     2701 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggggcggc
     2761 ctccggttgg gattactctt cccgttggct ccgtgatact ggtcgtctgg cgagcattcg
     2821 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     2881 cgccaacatc tcggcgctga aaggtgagaa agagacagaa gcactgttcc gccagaaggc
     2941 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     3001 cgattacgac tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccgct
     3061 ctatgtcggt atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tgcgcagtcg
     3121 gttactgaca cctggcggga ttctggcaag cgagtacgaa accggtgaac agtgggataa
     3181 acccaacggc tgggcaccgt tacaatggat ggcaattcag ggatttaaaa tgtatggcga
     3241 tgaccttctg ggcgatgaaa tcgcgcgcag ctggctgaag acggtgaatc agttttatct
     3301 ggaacagcac aaactgatcg aaaaatacca tattgccgat ggtgttcccc gcgaaggcgg
     3361 cggtggcgag tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt
     3421 aattggtttg tacggcgaac cataatattt ttacagccag ccgctaactt cctgctggct
     3481 gtaaaattat cctcttcagg aggagatatt taacatcatt gccgcctggg tgcgattttt
     3541 cacttccaga cggcgataca gggattccag atgcgctttt accgttccgg tactgatatt
     3601 cagcgctctg ccgatctcct tatttgattc gcccgccgct aacatggtta aaatctcccg
     3661 ctggcgggcg cttaacgatt tgagatcttt aatgtccttt tccggcgtcg tccgccagtc
     3721 tccaggcaga aacattatcc ccatcgccgc actatttacc gccaacgcaa atgtctcgac
     3781 ggttgaatca cgaggcacaa tggccagcac attaaaatgg ataacttcct gtaaccaccg
     3841 tttattgcag tccgtcgccg taattaacac cttaacctca ggaaattgca ccacggtttt
     3901 ttgcagcaac cagtagcaaa actcaccatc ctgatcgcca tcgagcataa ctaaggcttc
     3961 agggtaactt tccagctttt gccataactc gtctgcctga ctggccccct gaatactcac
     4021 tcctggaata cgctgctgta aactgatttt cattccatga ataaatattg actgcctgtc
     4081 aaacatgact atttgcataa ctgaatctcc acctgaatac gttaaaaaga cttaagtagt
     4141 ggaagggtat tacccgcgag aaaaaataag aattcgccat ttggcggtgg ccattctaca
     4201 gagatggcgc gtagaaaata gttaccgata taaatagtta cagctaaacg cctgaaatta
     4261 catgtcgagg gcactattta aaacaatttt gaggatttcc ttatattggt ggttagtacg
     4321 catgcaatta aaaatgaaat tccgcgacca caagccaaaa taacaaatgg caatgagaca
     4381 aaaataagca caaatagcca atatgtcctc tattcacttt aaagggaatc gctgaaaaat
     4441 acgctctgtt taaggggatt cacctttctc agaaagctat tccgcccttt tcctgctgag
     4501 aaatcgccac attcgtcatg acaacattgt gaaacccggc attagatgtt agaaaaaata
     4561 ataaacaatg cgatatgcgc gttactgttt cgctgtgaac aacaatcggt caaagaaatg
     4621 gataaaattc acgcaatgca gttgttcatc aaagtcgcgg agctggaaag tttttcccgc
     4681 gcagcggatt tctttgcttt gccaaaagga agtgtttcgc gccagataca ggcactggaa
     4741 catcaacttg gcacccagct tctccagcgc accacgcgac gggtcaaact cacgccagaa
     4801 ggcatgacct attatcaacg agcaaaagat gtgttgagta atctcaacga actggacggt
     4861 ctgtttcagc aggatgccac cagtatcagc ggtaaattac gcatcgacat cccgccagga
     4921 atcgcaaaaa gcctgttact gccgcacctg tcggaatttc tctatcagca tccgggaatt
     4981 gagctggaac tgagtagcca tgaccgtccg gtagatattc ttcatgatgg ttttgattgc
     5041 gtgatacgca ctggcgcgtt accggaagat ggcgttatcg cccgtcccct cggcaaactg
     5101 accatggtca actgtgccag tccgcactat ctgacgcgct ttggttatcc gcaaaccccc
     5161 gacgatctca cttcacacgc aatagtgcgt tatacaccgc acctgggtgt acatccgtta
     5221 ggttttgagg ttgccagcgt taatggcgtc cagtggttta agtctggcgg catgttgacg
     5281 gtaaacagta gcgaaaacta tctcgccgcc ggtcttgccg gtctggggat tattcagatc
     5341 ccgcgcattg ccgtgcgcga agccctgcgt gccgggcggc ttattgaagt gttgccaggc
     5401 taccgtgccg agccgctctc cctttcgctg gtttatccgc agcgtcggga gctttcccgg
     5461 cgtgtaaacc tgtttatgca gtggctggct ggcgtaatga aagagtacct ggactgaccg
     5521 actatacttt ttaagaatga ccacgacaga aggacaaaag agcggatgac gcaggaaaac
     5581 gagatcaaac gtcccaccca ggatctggag cacgagccga ttaagcagtt ggataatagc
     5641 gagaaaggcg gcaaagttag ccaggcgctg gaaaccgtca ccaccactgc cgaaaaagtc
     5701 caacgccaac cggttattgc gcacctgatt cgcgcgacag aacgctttaa cgatcggctg
     5761 ggtaaccagt ttggtgcggc tatcacctat ttctcatttt tgtcgatgat cccgattttg
     5821 atggtgtcgt ttgccgcagg gggctttgtg ctggcctccc atccgatgtt gctacaggat
     5881 atcttcgaca aaattctgca aaacatcagc gatccgacgc tagccgctac gttgaaaaac
     5941 accatcaaca ccgccgttca gcagcgtacg actgtagggc ttgtcggcct ggcggtggcg
     6001 ctttattccg gcatcaactg gatgggtaac ctgcgtgaag cgattcgtgc ccagtcgcgc
     6061 gatgtctggg aacgctcgcc gcaagatcag gagaagttct gggtaaaata tctgcgtgat
     6121 tttatttcgc tgattggttt gttgattgcg ctgattgtga cgctttcgat cacctcggtt
     6181 gccggttcgg cgcagcaaat gattattagc gccctgcacc tgaacagcat tgagtggctg
     6241 aaaccgacgt ggcggttgat tggcctggcg atttccatct tcgccaacta tctgcttttc
     6301 ttctggatct tctggcggtt gccgcgccac cgtccgcgca aaaaagccct gattcgcgga
     6361 acatttctgg ctgctattgg ttttgaagtg attaaaatcg tgatgaccta caccctgcca
     6421 tcgttgatga aatccccctc tggcgcagca tttggttccg tgctggggct gatggcattt
     6481 ttctacttct tcgcccgttt gacgctgttt tgcgcggcgt ggattgccac cgccgaatat
     6541 aaagacgacc cgcgaatgcc ggggaaaacg cagccttaaa ataggccgga tgcagttcaa
     6601 aacgccttat ccggcatacg aatttattcg gcctggctcc ccgtaggccg aataagatgc
     6661 gccagcatcg catccggcta taatgcgcac ataacctctt gaaactcttt cccagagcct
     6721 cttcagccat ctattcagga gcaaacaatt tcatgccaac tcataacccc agcatataaa
     6781 tccagttggt aacttttatt taacctgaaa ccagttttat ccactattta taaaattatg
     6841 tgaagcattt catagaagaa aaatcactgg cctaaacatt atcccctttt tgcctggttt
     6901 ttgaccattt ccgcgatttg ttacacattg aaatatcact tttgctgtgc gtaatatggc
     6961 tattcgttag ccaaaaaata agaaaagatt atgcaagcaa cagccacaac actcgaccac
     7021 gagcaagaat acacgccgat caactcgcgt aataaagtcc ttgtcgcctc tctcattggc
     7081 acggccattg agttcttcga cttttacatt tacgccactg cggccgttat tgtgtttccg
     7141 catatcttct tcccgcaggg cgatcctacg gcagcaacgc tacagtcgct cgccaccttc
     7201 gccatcgcct tcgtcgcgcg ccccattggc tctgccgttt ttggtcattt tggcgatcgc
     7261 gttgggcgta aagcgacgct ggtcgcctcg ttgctaacga tggggatttc gaccgtggtg
     7321 attggtctgc tgccaggcta tgccacgatt ggtattttcg ccccgctgct gctggcgctg
     7381 gctcgatttg gtcagggtct gggcttaggt ggtgaatggg gcggcgcggc gctgctggcg
     7441 actgaaaacg ccccaccgcg caaacgtgca ttgtacggct ccttcccgca gctgggcgca
     7501 ccgattggct tcttctttgc caacggcact ttcttgctgc tttcctggct actgaccgac
     7561 gagcagttta tgagctgggg ctggcgtgtg ccatttatct tctcggcggt gctggtcatt
     7621 atcggcctgt atgttcgcgt gtcgctgcat gagtcgccgg tgtttgagaa agtcgctaaa
     7681 gcgaaaaaac aggtgaagat cccgctgggt acgctgctga ccaaacatgt tcgcgtaacc
     7741 gtactgggta cgttcattat gctggcaacc tatacgctgt tttacatcat gacggtctac
     7801 tctatgacct ttagtaccgc cgccgcgcca gttgggcttg gcctgccgcg taacgaagtg
     7861 ttgtggatgt tgatgatggc agttattggt tttggcgtga tggtgccagt cgctggatta
     7921 ctggctgatg cctttggtcg ccgtaaaagc atggtaatca tcaccacgct gatcatcctg
     7981 ttcgcgctgt tcgcctttaa cccactgctc ggttctggca acccgattct ggtttttgcc
     8041 ttcctgctgc tggggttaag tctgatgggt ctgaccttcg ggccaatggg tgcgctgtta
     8101 ccagagctgt ttccgacaga agtgcgttac accggagcat cgttctctta caacgtagcg
     8161 tcgattctcg gggcttccgt tgcgccatat atcgcagcct ggttgcagac taactacggg
     8221 ctaagtgcgg tggggttata tctggcggcg atggctggct tgacgttaat cgccctgctg
     8281 ctgacccatg agacgcgaca tcagtcgttg taataagagt gaaaagcctg atgcgctacg
     8341 cttatcaggc ctgaatttcc atacaatata ttgaattcgc aaggatttgt aggccggata
     8401 aggcgttcac gccgcatccg gcatgaacaa agcacacttt gccaacaatc taaatctgtt
     8461 taattgccgg atgtcagaca tccggcaatt atcttcactt cttcatctgc gacaaaatcg
     8521 tccggcactg attagcctcc ccttcggaag gtgagatcag tgccagcaac gctgccgctg
     8581 gcgtaaccag cgttgccagt gccgcagcaa cagcaccacg ggcaatcaac ggcccggctt
     8641 tcaccccagc ctgaggattt ttaaacgtcc cacgcacata cagcggcgaa cgcagtgtga
     8701 taatccgaat acctttactc tccggatcaa tagtcaaatc cagctgttcc gaagcaaaac
     8761 ttgccgtgcc ggtaacgtta atcaacgcgt tctcagtatc gaaagcaaaa atctgcgggc
     8821 gtgccacgcc gttggcaata ttcagattcg ccgccgcgca gttcacccgc acctcatcgt
     8881 caccaaatat cgcaccgaca atgtagttgc cgacattcag cccaacaatc tccatcaggt
     8941 tgcggctcac cagcccgtca ttcatcaaca gtttcaggtt gccgttactg ttgcctaaaa
     9001 gtgccgccac cgagttaccg ctaccgcgca gttccgcgtc accgttcatt tcccccagcg
     9061 tcttctgcat cagttccaca tcgggcatca gttctttcag tttcagtcga cgagcctgaa
     9121 tatctgcccg cccctgcatc ggctttttat cgccttccag atgaatattc gccgcaatgc
     9181 tgccacccgc aatgccaaat ttcagcggtt gcaggcgcaa gtcagcattt ttgaggatga
     9241 tatgagtaga aagatcgcta atcggcaggc tactgccatg ctcaatgcgc cgccctttga
     9301 agcgaacatc ggcatccata acgtcccatt tatcggtttc gaagcggtca taaggcagca
     9361 ctttgcccgc aggctgaacg cttttttcgc ccttcttctg ttcagaccgt ttcgactttt
     9421 ctgccccttt cccggaatca acgccaatca acggtcccag gtccgccagc cgcaattgcc
     9481 gcgactcgac atcaccttcc agttttggtc gtggcttgcc ggtggtgtag accagagaac
     9541 cgtggatatc gctatcgcca attcgcccat taaagccgcg ataatcaaag accgacgatt
     9601 tttcagtgtc gattttcgct accagccgac catccgtttc aaacggcggg gtatcgggca
     9661 acagaacgcc cgtcagttca tagagatcac ccagtgaatc gccagaaaat ttaagccgta
     9721 aatcgacacc gcccatcttc attgggtcat tcacgacgcc atcaaaagca acacgggtat
     9781 tacctgaacg gaaatcagcc tgtaccggaa acggcgtccc ttcgccacgc agcgccagca
     9841 taccgcctat ttttcccgta cccgtgagcg gttcaccgtt ataacgtccc tgcgccttca
     9901 ggccaaaaac gtaatcgccc accttttctt tatccgcttt acctttcgat ccagtaactt
     9961 cgctgaacgg cagcggcttg cctaagggat caacaaaaat ctccagatcc gctttgctta
    10021 ctttgtcatc aatggcgatc cgcccttgat cgaaaagaat attatccagc cgaaacgacc
    10081 atgccgacgg ctttgcattc gcgtctttgt tatcatcgtt ggcgagatta aacgtccagt
    10141 tattgttctt ttcagagagg cgaatcaggc gcgcgtcggg cttttcgagc ttgatccacg
    10201 gcagccagac cgttttggtc agcagcgcca gcggggccag cgttgcttct acgcgtggca
    10261 aatgcaccat cgtgacttcg ggaatatccg gtggattgcc aagaatgatg tcttccgcgt
    10321 gtacatgggg ccacggcacc cagctgcgcc agccagtttc ttgcttttgc cgctcccaca
    10381 ccacgcccag atcgccacgg atagcgaacg gacgattcaa ctccgcagag actttctggt
    10441 tgatggtcgg tttgagtcga ttccagtcaa aagttgcaat caaaatgatc gccacgacaa
    10501 tcaacaacaa gaaagcccct gaaatcgcag cggttatttt gcctgccttg ctcatcgttc
    10561 gcttcctcat attcttcctg tgccagtcct aaagatagtc cagccaggcg gaaaatgagg
    10621 cagcttatag cgccagaacc gccgtattca gcgtttctat cggtgccggg cgtgaaagaa
    10681 accagccttg tgcggcgaat gcgggcgagt tctgtacatc acgccactct tccggcgttt
    10741 ctacgccctc gacaatcacc ccgcgacaat agcgattcat tagatgtaaa agctgagaaa
    10801 agagtgtgcg tccttccggc gactgacgca gcatcacaaa cagttctcgc gcgattttga
    10861 tgtagtcata acgcacttca cttagtgcag agaaatttgc catcccggta ccaaaatcat
    10921 ccagccacag cgggccaaat tcacacatcg aggcaaaggt tgaatctttc ggcagacgga
    10981 tatgctccac cagttcgaaa cgcagccagg gaagacgctc aatctggcgc aggatttttg
    11041 gttgctgacg cagggcgatg agcgtaggac catcaatatt gaccgatgcc agcaggccgt
    11101 gctctataaa gaagtcggct ttttgcgcca gcaaatcaat ctgctctttc acaacctcca
    11161 tccgatggct gacggtgatt tcagtaaaat agcgatccgg cggcaggcgt tgcgaagggt
    11221 tcaagggatg cgtgaccgcc gttaatagct ccacggccat taaccgcccg catgtttgat
    11281 agatcggctg ccaggtgtaa gcacgctcac actgcaacca aaaacgccgt tcctgcaagc
    11341 tctcgatgct tgcttcaggg ttgcttattc gctggataac ctgccttatc atcaaagatg
    11401 tcctgtgttt aaaagtgatg cagcccggac tcgacaaaga ttatcggcgc gcaagggcag
    11461 aactttaccg cctggccggg gtaaatttcc ccactccaca acggaatctt tcaggaatac
    11521 gttctggctc aaaaaaataa tggaacactg ttttaatatg gttgaccagc aaaccaccac
    11581 agcgcaaact aacgctaatt ttttacagat caggttcacg actatgtcca aaaagattgc
    11641 cgtgattggc gaatgcatga ttgagctttc cgagaaaggc gcggacgtta agcgcggttt
    11701 cggcggcgat accctgaaca cttccgtcta tatcgcccgt caggtcgatc ctgcggcatt
    11761 aaccgttcat tacgtaacgg cgctgggaac ggacagtttt agccagcaga tgctggacgc
    11821 ctggcacggc gagaacgttg atacttccct gacccaacgg atggaaaacc gtctgccggg
    11881 cctttactac attgaaaccg acagcaccgg cgaacgtacg ttctactact ggcgtaacga
    11941 agccgccgcc aaattctggc tggagagcga tcaatccgcg gcgatttgcg aagagctggc
    12001 gaatttcgat tatctctacc tgagcgggat tagcctggcg atcttaagcc cgaccagccg
    12061 cgaaaagctg ctttccctgc tgcgcgaatg ccgcgccaac ggcggaaaag tgattttcga
    12121 caataactat cgtccgcgcc tgtgggccag caaagaagag acacagcagg tgtaccaaca
    12181 aatgctggaa tgcacggata tcgccttcct gacgctggac gatgaagacg cgctgtgggg
    12241 tcaacagccg gtggaagacg tcattgcgcg cacccataac gcgggcgtga aagaagtggt
    12301 ggtgaaacgc ggggcggatt cttgcctggt gtccattgct ggcgaagggt tagtggatgt
    12361 tccggcggtg aaactgccga aagaaaaagt gatcgatacc accgcagctg gcgactcttt
    12421 cagtgccggt tatctggcgg tacgtttgac gggcggcagt gcggaaaacg cagcgaaacg
    12481 cgggcacctg accgcaagta ccgttattca gtatcgcggc gcgattatcc cgcgtgaggc
    12541 gatgccagcg taaacgcagc atatgcaaaa ggctggataa agagttaccc ctctatccag
    12601 ctcatccata aatacctgat ggttgtgcta actcatcacc atcaggcatt actgtgcagg
    12661 tggaatatcc gtcacttcag gatgtacgtc atccgtggcg acagaggtgg tcgcagcggc
    12721 ggtagatggg gccatgattt gatcccaggc cgcctgcaac gctttcatgt taaattccgg
    12781 ctcgcctttc ggctgcagca gtattaacgc catatcattc gacaactgct gacgcagatc
    12841 ctgatttaac atctccacag tcaggctgtt aaggaaatcc tgccgcagtt tctgatactg
    12901 ctccggcgcg atatcgacga cctgattttg caacgaacgc atccgctggc ccatcagaat
    12961 atcggtatca gcccgtgcat aggcggcaaa cagtttctgc agctccagtt tcttttgcgc
    13021 cactaacgca ttgaactctt cttccggcag acctttatcg cgaaccttcg ccagttcacg
    13081 cgccaccaga ttaaggttgc tgttcagctt gtcgtttggt gattcgatgt tgatggcaca
    13141 ctgcgcacgc agatacagca cacggcagtc aaatccaaga ccgatgtctt tgctgttact
    13201 ggcgcttaac gcttgctgaa catgccagaa cagcgcctca cgggccaggt ccgcacgcca
    13261 gtagcgcagc agtgcggctg attcacgaat cggctgccac ggcgtatccc acatgataga
    13321 taaccggtcc tgacgcaccg cgtcagtcat aatactcacc gcttccgcac gcagcggaga
    13381 aagcgtcggc accggagccg gtgtttcacg tttgcctttc agttcgccaa acgttttgtt
    13441 gatttggtcg acgaccgagc gcgcatccac gtttcccacc accagcagcg tcattgcatc
    13501 cggggtgtac catttctgat agaaatcttt aattttttcc gcttcaacgg gttgtttcag
    13561 cggatcggca ggatcgtgac ctaacaaggt tgaccctttc agacgatagc gccaccagcc
    13621 ctctttagta tcggcaggcc aggttgccac catgtcctga ctttgcagcg cgtggttgat
    13681 ggtttctggc gtgatagtca gtttgccagt ggcatttgcc agataagaga gcgcctcttt
    13741 cagcaaatcg ttacggttat tgggcaaact cagattaaac agcgtggtgt cataagagac
    13801 aattaccggc ggcatcgggc gtttagggtc gatcccctgc tgccacaatg aacgcgcctg
    13861 tgctgcgtca aggccaccgc tttgcgttag cgcaatacga gggatggcgt gactgtaacc
    13921 gctctgttgt gtactttcgg cgagcgaacc ggtattaacc agcaggcgaa tttcaacacg
    13981 atcgctggga cgctgggggg tggtcagcac ttgccactgt aaaccgttgg aaagcgtccc
    14041 ctgttgccat gctggatcag gctggagcgc atctgcctgc acatagccag cagtggccat
    14101 catcagcaaa ccgcccgcta aaagtcgaat ttttgtgccc tgcatgtgaa cccctgatca
    14161 actatcctgg taataaaaaa actgcccgtt gtcggtcagt ctaaatgacg ttaaaaacac
    14221 ttcgtgttag accgcaagaa catgaaaatg tcacggaaga agtgaaataa acctgaactc
    14281 acccaggtgg taagttcagg cacaggggtc aattatgcgc aaacacccgc actcggggaa
    14341 gggagtgcgg gcataagtga tgagattaag aggataattc gtgcgttttg ccatccggcg
    14401 cacgattatt cagcgcatcg tccagttttt tgtggtccag ttctttcacc cacttagcaa
    14461 cgacaatggt cgctacgccg ttaccgacca ggttagtcag cgcacgagct tctgacataa
    14521 agcggtcgat accgaggatc agcgccagac ccgctaccgg caaatggccc accgcagaga
    14581 gcgtcgccgc cagcacgata aagccactac ccgttacccc tgccgcccct ttagaagaaa
    14641 gcagcaacac gattaacagc gtgatttggt ggacgatatc catctgactg ttagtggcct
    14701 gggcgataaa caccgccgcc attgtcaggt atatcgatgt gccatcaagg ttaaacgagt
    14761 agcctgtcgg gatgaccagc cccaccaccg atttacggca gccgagtttc tccatcttgt
    14821 cgagcatacg cggcagcgcc gactcggaag atgaagtccc cagtacaatc agcagttctt
    14881 cacggatgta gcggataaat ttgaagatac tgaaaccagt cgctttagcg attgaaccca
    14941 ataccagcac cacaaacagg atacaggtaa tgtagaaaca gataatcagc tgccccagtt
    15001 gcaccagtgt gccgacgccg tatttaccga tggtaaacgc cattgccccg aacgcaccaa
    15061 taggtgccag acgcatgatc atattgatga tgccgaagat gacctgcgag aaactttcga
    15121 tgacgttaaa aatcagttgg cctttgctgc ccagacggtg gagcgcaaaa ccaaacagta
    15181 cggcaaacag cagcacctgc agaatgttac cgctggcaaa tgcgccaatg acgctcgccg
    15241 ggatgacatc cataatgaag gcgacaatgc cctggtcttt cgcctgatcg gcgtaaaccg
    15301 ctaccgcttt cgcatcaagc gttgccggat cgacgttcat tccggcacca ggctgcacga
    15361 cgttaacgat gataagacca ataatcagcg cgatggtact gacaatttca aagtaaagca
    15421 gtgcgactgc gccggtacga ccgaccgcct tcatgctttc catgcccgca atgcccgtta
    15481 cgacggtaca aaagatgaca ggagcgatga tcatcttaat gagcttaacg aagccgtcgc
    15541 caagcggttt catttgctcg cctatttcag gatagaaatg gccaaggaga ataccaatgg
    15601 ctatcgctgt caggacctga aagtaaaggc ttttaaacag agaggttttc atagggtgtc
    15661 ctttagtaaa accacaggtc ttgtaaggtt atggggtacc tgcggcctta aaataacacc
    15721 cagacaacat cacagaaatg tacctggatc ataattgaaa caaaaaggtt aaaaagtttg
    15781 agctggctcg cacaaaccag cactttttaa agttttgtaa tcagtttggg gtagctactt
    15841 ttcttccagg taactctctt cgaagatttc aatagggagt ggacgagcaa aaaggaagcc
    15901 ctgggcaata ccaacgcccg cttttgccag ccagtcgcgt tgtgcttcag tctccacgcc
    15961 ttcggcaatc atttgtaagt tcaggctctg cgccagcatg ataattgcag caatcatgct
    16021 gctatcttcc ggcaagcctt caacaaacat tttgtcgatt ttcagaacgt cgattggcaa
    16081 cgatttcata tgctgcagct gacgcagccc tgcgtagccc atgccgaaat catccagcgc
    16141 cacccgaact ccggcattac gcagcggacg gaggattgcc accgcagcat gagggtcgtc
    16201 aatacgtcgg ctttctgtca cttccagaat cagtgttccc ggctgaatgc gatagcgggt
    16261 taacagttcc agcatatccg ccaccatatt cgggtgcatc agttgcagca cagagaggtt
    16321 taccgacaag ggcagcataa tgccgcgctc ttgccaggct gcaagcaatc gacaggactc
    16381 ttccagcacc cagtgaccga cggtaaccat cagcccacag cactcaatgc gatcgattaa
    16441 gccatccggc aggtcccaac tgccatccgg ttgctggata cgcagtaaca cttccgcact
    16501 gaccagttta ccgctggtca tctcgacctg tggctgtaac caaatagcaa actgatgatt
    16561 ttccagtgca ttaaggatat cgctctcttc cgtcaaccgc ttctgggcgg cttccatctg
    16621 ctgcggatca aagaactgaa tctgattctt gcctttatgg cgagcggtaa atgccgcaga
    16681 aatagcgcga ctgtaaagct gttcggcggt gagatcgccg tagaacatcg ccacgctaat
    16741 gctacagtgc ggacggagtt gaatacgttc aatcggcagg cgctcgctca tgatagtgag
    16801 cacttgctga cccaaagtga ttgcgtgcca cggttcatgt acaccgttgg caatgacagc
    16861 aaagtcataa ccgctaatct gcgcgaggat catacgtggc gacagtaccg atttgagttt
    16921 ttccaccagc gtcagcagca gaatttctcg ttgcgcctct ttcagcacgc ccgcagtatc
    16981 acgcagagtt tcacaggtga tgatcatcag cgcggtggtt tgtttacgcg cgacaacctg
    17041 ctccagcatc tccatcagca aggctttgtt cggcaaatcc gacaccggga agcgcatcgc
    17101 attttcgttc tgttcttcat aatggcgctg cagcaattgc tggttgaggt tgtaactgcg
    17161 caccaacata ccgatttcat cgtcctgatg cagacgcggt aatgccagtt ggtgaccaac
    17221 aagctcctgg gctgggatgg cgttaagttc gcgagcaata ttgcgtaacg gatgcaaaat
    17281 caggcggtta atgcaccagc taatcgccac cgtcaatatt aacgacaaaa gtaagtaaat
    17341 ggtcactaac gttgagaggg tgctcatcac gaacttatac atacggaagg aatccgcctg
    17401 tagcaccaga taggcaattg gctgcggatt tgccggacgt tcgagcgagt agacgcccag
    17461 cgagatttga accggtagct caaacaggcg agtaaccatt accggcaccg gacgctctgg
    17521 aataaaactt ttacgcagcg cctggaactg gttaggcagc actacatcgg cacggctgac
    17581 cacgcctgcc ggtttaatgc tggcaagaat agcttccgct tcgggaatat cacctttcaa
    17641 aatggctgaa gataaaggtt cgcggacaga gcgagcaatg ctttccagtt gcgtagccgt
    17701 gttatagcga ttctgctgga ccagatggaa cagcaaaacg gtgcaaaaaa taaaaacgaa
    17761 caccaggaca acggctgcca ccattgccat ctgcttgatt gttaacgagc gacttacgcg
    17821 caaattgtct ccccccggaa atcccagatt gccgcccgag tatacccgat cgcagcggca
    17881 ttaagagagg cgctatctga aaacttacca gtcggcgtaa ggtatcagcg gctgcggcgg
    17941 taaatccatg tcaccctgcc atccggcggc ggaataacgt acgtagagca ggaaatggct
    18001 gggggcgtaa tctttcgcct gctggatatc aattgccgtg ccgacaaacc agttggaagt
    18061 aacacgtcgt tcaagtaatg cccgcgccgt atagccgaag ccctgactgc tgccgccatc
    18121 gttggattgc cgcgcagctt cttcctgcca gtcggtcggg atcagattca tcagcggata
    18181 acgcggcatg gttttggtgc gtgaatgcga ccacgagcca gacgccccca gctcccacga
    18241 ccagttttcc gtgcgctccc gccacatcac cggtatggca aacgacaggt attcctgcgg
    18301 gctgtagtaa ccgccctgac cgagtgagta gccactcagg tctttgtcgt aatgccagat
    18361 catgttgttc aggccgattg tgacgcggcg attgttctgg ttgatgacct tatagtaata
    18421 gcccgtcatc cagcgcacac gccagttatc ttcgacattt ttaccggtta actggtcgcc
    18481 actaagcgat gcccagacgc cgtttgcttc acctttatcg tagctcaggc ttagccccac
    18541 gccgtcggca cgcacgccgc cccatttttt cccggtattg ctcgggaagt ctttttgccc
    18601 accaaaggcc agcaaagaac tggagatggg acgacggtgg gcgttaacgg tgtaacccag
    18661 cggcccgata tcatcgctgt aactgatgcc gccgaccaca tccaccacgt tgaagcccat
    18721 cggcgtggtg ccgatatccc agctccagac gtcatttcgc cagccgaccg ccacgctggc
    18781 accggaatcc gactggctgc ggttgccgct acagtcccgt aatgtacagg tgccccagtt
    18841 gtcatcccat ttgccatcgg cattagtgga gaaactgccg acgttcatat tgacgaaatc
    18901 actgcgaaag aacatccgcc cgtcagaata cggcgcatcc acctgcaaca tggtagtgtg
    18961 cgctttcaga tcggagtaac caccggtgcc gctcgaaccc caataatcat gctcgagggt
    19021 gacgttaaga tcctgctggc gatagaggtc cgccgcatcg ctgcgcacgc cgcgtttcag
    19081 ccagtcatct ttctcgtcgt tacgggtcag tcgggtaaag gtgtcgttat cctgcggacg
    19141 cgtcgtagtc acaccggatg cgaccatggc gtctttgtag gtttccagcg cctgctttgg
    19201 atcgcccgcc tgcgcttcaa acttcgcacc gtcgcgcagc accatcgcgc tttccatcga
    19261 cggtggctga gattttgcct gcgggatcaa cttattaaac gtctgctgcg ccgctgcggt
    19321 atctccaagc tgcgcctgcg ccagcgccac gcgccgctgt gtgttcagcg aggcgttatc
    19381 agtagcgggc agtttcgcca gctggctacg tgccgccgct gtgtcaccgg cagcaatatc
    19441 cacttccgtc agaccaagaa tggcatcggc gttagttggc tcccgcgtca ggacattctg
    19501 atatgcagcg cgggcggcgg tgtaatcacg tcgctgcagc gcccagtcag ccagcgtgag
    19561 gtcaatacgc gtggaaggtg gttgctgacg cagcatcgct tccgcttcag cttctttgcc
    19621 gctttctcgc aggcgattag cggtttccag cacctgatcg ctttgcagtc gattaaccag
    19681 ctcctgaata ttgctgctcc actgcgcacg cggcaggctg ttgatatgcg ccagcgccgc
    19741 tctgtcctgg tcatgaccag agaggtacag cccgtaagcg taaacctgct ccgggtcgtt
    19801 cggcttctgc tgcgccagat tgcgcattaa cgtatcggcc tggctgcgtt gtccggcctg
    19861 ccagagatcc tgcgaaagtc ggtaagtaat ccatacgctg ccggggtcca gcgccagtcg
    19921 ttgccgctga agtgctgccg cctgcgccca tttgccctgg ttttccagtg cctctgcctg
    19981 ctgtgccaga cggtcgtttt gcaggctgcg ttcgatatca tcaatgctac gccgctgact
    20041 ggcagagagt gaggcgataa acgcttcagc tttttctggc gattgctggc ggtaaatatt
    20101 tgccagcccg cgcacggcgt tagtgttgcc gctgtccata cgcaaggtct gctgataata
    20161 acgttcggcg gcgggataat cttttcgcgc catcgccaca tcgcccagcc ccagcactgc
    20221 ataactgtcg gtgttatcga cattacgcgc ctgctggaac aggcgttctg cccggtcagg
    20281 attattggct ttcagcgcag catcgccctg ctggatcgcc agccagtagc ggtttacttt
    20341 cagcagactg ttccatttgt cgttgttgct gctgtgcgga tccagtgcga gggctttttc
    20401 cagattcgcc actgcattgg cgcgatcgcc tttctgtgaa tatgcctggc ccagcgcccc
    20461 cagagcttca ctgtctttcg ggtttgcccg cactgcctgt tgtagttcgg gaatggcttt
    20521 acccgccata ccagagtcca ccgccgctaa accttgcgca cgagcgcgga aagcaggatc
    20581 ggccagctgt ttttgctgtt ctgccagttg cgattgcgca gccgccacgc tatcgccatc
    20641 actaaagata gaaagatatt ttttcagcgc cgacacactg gcatcactga cgggcatgtc
    20701 tttaatctgc ccgtaccaga ttttagaggc cccttcgcgc ccggcgttcg atttcgccat
    20761 ctgttccagg acggcaaaac cttcgtcacg gcgatcgcta ctaaacagca atagcgccag
    20821 attgttttgc aggcccgtat tgcccggttc atccgcattg atgcgtttta actgattaat
    20881 cgcttcgcca cggcgagccg gaattttcgc caccgtactc cagtactcga cagcaatgtc
    20941 accttccggc ggcgcaccgt tgaacagttt gttgtaactc gccacagctt cttccgcatg
    21001 accggtagtc gcctgcaatc ttgcctgttg cagtgcctga cgaccatccg gcgtggaaag
    21061 tagcatcgta gtccgcgacg atttatacgc atttgaactc ggcgctaact gcgacagccg
    21121 atcgagctgt ttttgcgcgc catcaatatc gccctgacgt aacaaagaac ggaaacgggc
    21181 ggcaacgacg tccgggttat tcggatcaat aagttccagc cgatacaacg actgctgcac
    21241 caggtcttca cgatgggtcg cttcgcctaa ccgaacttgc tccagcaact gttgctgagc
    21301 ggttggtgct gcctcgacca tcggcatgac ggccagaccg agggaaagcg tgaatatgtt
    21361 tagtgtgaat ttgcgcattc ctggccccag tcaggtaata actcaccttt tgtcgagaag
    21421 cggaaacggt gttgatccca gccttgtcca aacagggtca gcacatagtt gtaataggca
    21481 tcgctgccgg gaaagttatc ggccacgcgc tggcgctgaa cggcctgcgc atcacggttt
    21541 tgtaaaaagg gcagcatggc ggcagaaaaa ccgaccggtc ctttaccctg cgctttcccc
    21601 gtagccacat ccactttttc cggcggataa ccgtttttct cagtgaatgt cgccatcggt
    21661 ttaaaccggt tgagcatccg cgctttctgc ggatcgctgt caggcatcat gcctacccac
    21721 atgtaaacgc ggatagcgtc gtagctgctg atcaatgttt tttcggcttt tagctgccag
    21781 cctttgtctt tctcatagcg cacccagtct ggcgaaaagc ctttcggggc ggtttccagc
    21841 aataaacgtt gattggtttc gcgcagcgta gtccacggcg cgccaaagcg ggtgaaatac
    21901 tgcgccagcg tcggcggcag gtagctgggg ttaaaacgcc agctgttatc ctcagcaaaa
    21961 cccactttgc ccggtaacaa catggaaccc agcccaggca ccgtcaccac ttcctcccgc
    22021 gcaatacgtt ttagcaacgc gctgccgatg tcggtataac gctgctcttt ccacaaacgc
    22081 cccgcctcca gcaacgacca agccatccag acatcaccat cggaggccga attgctgtcc
    22141 agcacttccc acttactgtt ctctttcttg ccccacagcc aggcgggcaa acgttctttt
    22201 aaagaaccct gagcgagatt gttctgcgtc cagtcgagaa tattatcgaa agctgcacgg
    22261 tcgttagccg ccagggcaaa gaacatgccg taactttgcc cttcagaggt ggtgattttg
    22321 cgcgcgtcgc tggggtcgat gacgcgccct tcctgactga tgtaatcctt tttaaactgc
    22381 tcccaggcag gccaggtaca ggctgcctga acactaaagg cagccaacag cagcatcgtc
    22441 acgagtccac tacgcaacac attcatcttc aattactcgt tatccgggtt aagacgacga
    22501 cggctgataa tacgcagcaa acgccacagt acccatgcca gcaatatcac actgatagcc
    22561 gccagcaccg ccagcaccgc cagcaccgcc agcagaatcg gatggtttgc cagcgcatac
    22621 cacaagcgct cgaaccacgg cagatgacct acgtaataaa cgtcgccaac acgtaggctg
    22681 ttgataccgg actcgcggat caccgcgacc gaaccgaaca tggtggcgcg tttgccgcta
    22741 tcgttcaccg catcgttaag catttcataa ccgcgtgggc tatctgccag cagcgcaatc
    22801 acgctgcgct ggtcgttata cggcgactgg aagccaatca ccgccgccat cgcaccggaa
    22861 gaggtcagcg ttgactgggt ttctgccgcg cgatcgctct cgtccggcac aatgccgggg
    22921 aacggggtct ggcgcatcgg tgttttcacc cagctttcgg tcgcctgcac caataggtcg
    22981 atctgcttat cgtctttcag tttgtccggg ataccaccga tgatcatgat gtcggcatct
    23041 ttgccctgaa tggtgctgcc atcatcggtc accgtcagat taatcgccgg gaagcccgtc
    23101 tgtgcgccga taaaaccaac agtattcagc aacgtttcca tctgtgcttc gttaggcgtt
    23161 ttcggcatca cggtgatggt ttgcgacaga tccgccatcc ggctgaacgg gaagcccgcg
    23221 ttagcaaagg cgcgtagatc cggcatcggg atgaagtggt aatacttcga gaagtcgatg
    23281 gtggagtcgt caccaatcac cacatgattc tgcaccggct ggaaggtaat acagttatcc
    23341 accgaaccgc caggcatcgg gttcatatac tcaaagtcga agcgcagctg gttggtcgcg
    23401 cccagtttca gcgccggaat agagacatcc gttttgccat ccagcaaacc ttgtaatacc
    23461 ggaatccgca gcagcaggcg gttcgcctcc tgtttgctgc tcaggttgaa ggattgcagg
    23521 aactggttat tcaggctgat atccatccgc gaactgtctt tcaccggcgg catggtgtag
    23581 cggtaattaa tatccatatc aatgccggta ctgcgcatca ggtagagatc tggcggcagg
    23641 tttagcgaaa cattaatcgc tgctggctca agaccgctgg attgtaactg ttcttcatag
    23701 gttttcagtt caccaaaggt gaccggacga tcggtacgca cccagttcgg cgcatcgtac
    23761 ggcttacgcg gtagcagcgg tttcacttca ttcactacca cgctttcacc acggaacaga
    23821 atgttaccct gagcgatacc tttcgctgcc tgcaacaggt ctttgtcgtc acgaccaaac
    23881 accaccagca gtttgacgta aggattctgc ggatggttaa tcatctcaat caccggggct
    23941 tttaccgccg gatgatcgcg caggaagtcc ggccgtttgt cgttggttgc aaagacaatg
    24001 gcattacgat ccggcagttg gttatagagc accgggaagt tctgcccacg ccagccagaa
    24061 cgcgaaccaa accacgaggc gacaatggca gatgcttgtt gcagcccaac atccggcgca
    24121 cccgcaaaga ccatcggcaa ggtgttggtg cggttatcac gcgggtcaaa gaatggcacc
    24181 gggaagtgtg acaggtcatt cttcacattc agggtctgat aggtcagatc cagtccactg
    24241 ctccgcccaa catccagcca aagcgtggtg ctggccgggt tttcgcacac gtcctgataa
    24301 tggccgacaa actccagccg cacacggttg aagtcggtaa taaacagtgg attaatgggc
    24361 atttgcgcca gcgttttttt acccaactgt tctttggtca ctggcagcac gcccatcagc
    24421 tcatcattga gataaacctt taactgcgac tggacaggca gtaacgatgg cgatggcgtg
    24481 tattcgaggt tgagcatcgc cttcgtcacc acttcatcgc tgcgcatacc aaactcaatg
    24541 ctgccgttcg ggttaatgcc acgtagcacc atgctgcccg gcggcggcgc aatttgcgca
    24601 aaggtcagct tcacatcacg cgaaggaccg ttctgcgcca cgactggcgc atcagcgccc
    24661 tgcacgcccg gcatcacttg ccccacctgc ggattttgtt ccgtctgggc ggctacagct
    24721 ggctcagcat tgatcagtgg ttgcgttgct ggcgtcgcct gcgtcatgaa agaggggaac
    24781 gcactcatcc ccatagccac tgcacaaatc cagaatagtt ttcttttcat cgcgttatca
    24841 tcattgttga gccaaagcct gatccgatgg ttgtgccgtt tcgctccgct ccgggcggcg
    24901 cggaataaac gatacaaccc aggaaaccag agaagtcagc acacggaata tgcccttcac
    24961 cgaagaaggc gcaaactccg ccagatggcg gtagccacgg aagccgagct tcagaatatc
    25021 cagcagactt tccagcggct tatcttccgg gtagctgtcc tgccagagcg cccatgtatc
    25081 cgcacgggca aacgtacact gcacaaaatc gatatgttgc tgggtggtga gcggcattaa
    25141 tttcagccca acttcattac ccatcacgcg cgccacctgg gtcgggaaga cgtattcctg
    25201 ctgaccgcgt ttaagcaaca gattcacttt ctgcccttcc agaatctgcg cctgaccgtt
    25261 gatcttgatc cccaaaccac cgtcggagaa atcctgaacg gtacacgaga agaggtgacc
    25321 atcttcgcgg gcaattgccg cgggcatcgt catctccacg cggtgcgatc ggcgtacctg
    25381 tttgctttct accgataccg caactgcgcc gccaagaaca atcaggttgt agaataccca
    25441 caccatactg acgaccacgg tgagcatctc ggttggcggg ccatagaagt agcgccagat
    25501 gcctaccgca acgcccacca ggttgagcag aacaaggaag atgtagggcc gcgagatcac
    25561 ccagtcgacg tactcttctt ccaccagtcc acctttggcg gtgacgttaa atttgccttt
    25621 gtgcgggtta atcagcgcca ccagcgtcgg tggtgcgata taccacgcca gcaccgtttc
    25681 gtagatttca ctccagaaag agtggcgata tttgccctgg atcttggagt tggtcaggct
    25741 ggcatggatc atatgcggca gcacgaacag ggcgatcatc aacgctggcg catagatgat
    25801 gtaggcatga agcagcagga acgccagcgg cgcagtcagg aagatcagcc gtggaatgcc
    25861 cgacaagaag tggaacatgg cgttgacgta gcacagccgc tgagcaaact tcagcccttt
    25921 accggtgagc gggttatcga gacggaagat ttgcaccatc ccgcgcgccc agcgaatacg
    25981 ctgaccgata tgcgccgaca gactttcggt cgccagcccc gccgcctgcg gaatacgcat
    26041 atacgcggag gtatagccac gacggtgcaa acgcagagaa gtatgcgcat cttcagtcac
    26101 agtttcgaca gcaatgccgc caatttcatc cagcggctta cggcgaatca ccgcacagga
    26161 accgcagaag aaagtggcgt cccacatatc gttgccatcc tgcaccagac catagaacag
    26221 cgtgccttcg ttcggtgttt tacgaaaacg ccccaggttg cgttcaaacg ggtccggcga
    26281 gaagaaatgg tgtggggtct gcatcatcgc cagctgtttc tctttcagga accagcccac
    26341 ggtcatttgc aggaacgatc gcgttggtac gtggtcgcag tcgaaaatcg acacgaactc
    26401 gcctttggca tatttcagcg cattgttgat gttgcccgct ttcgcatgtt catgagtggt
    26461 acgggcgata tacttcaccc ccacgttttg cgcaaactgg cgaaactctt ccctgccgcc
    26521 atcatcgagg atccagatgt tcagcttgtc tttcggccag tcgataccca gcgaggcgta
    26581 aatggtattt ttcaccacgt tgagatcttc gttgtaagtc gggacaaaga tatccaccga
    26641 cggccacagc gacatatctt tcggcaatgg caccggctga cgattcagcg gccatactac
    26701 ctggaagtag ccgagcacca gcacaatcca cgcgtacgtt tcagcgaaga gcagaataag
    26761 cccgcacacc aggctgaccg gatcgtccca gttcagcgta gaggtatatc gccaccagat
    26821 ataacggcaa gaaacagtca gcgacagcac aatcaacatt agcgctgaga agcgccccgg
    26881 catccgccgt acgatcagcg ctccccccca cagcagcatc aggaagataa actgcgccag
    26941 cgggttaaac ggctgagtaa cgcagattaa cgccagaatc agcgagaagg tgacgatgat
    27001 accgaggatc aaccgccgcg ccccggcact caaatgaccg agctcttttt tctcatcaag
    27061 atgctgtgtt ttatggctaa cgcgctcagg cagctcgttc atccattgat ggtaacgtcc
    27121 acgaatattc tgcagacctg aaaatgcccg cctgcgcggt ttcggcgttt ctttgcgcga
    27181 tgcaccgatc agtaaccagc atgtttgaat gagataacgg accgggtcca gcggacgcgg
    27241 acgcgaggcg ttgatatgcg gatacaggtt tttatgttct gcgcgaatac gctgccagcg
    27301 cgggtgctcc agtggaataa aaatccaggc caggatcatc cagaaacagc cgagcgtcgc
    27361 gctgaaagcc gacgcaccgt gacgacgata atcgcgataa cgcccgataa gccgcgcgtt
    27421 gaccggcggg ataagcaacc accgggtcag gatactcatg atgcactccc gactggcgtt
    27481 ttcagcccgg aatagttcaa caggcaccag ttcgccagcg tcaatatctc ttcagccgcc
    27541 agcgcatcac tgcgatattc acctactggt tgcttagccg ccaggcattc agccatcgct
    27601 tcatcacgat gaatgagcat cggcagtaat cggcgctggc tttgcaacca aagctggtaa
    27661 atatcgtcct gaacctgact gccaatacgg aagttattaa tcaaaatatg tgcgccatcc
    27721 ggcagcgctt gctgatgcag tcggatatgg cagttggcat ccacattgac gattgccagc
    27781 gagtgatcgc acaaactcag cagctggtgg gttatctgcg aggcatcacg cggtaagtcg
    27841 attaaaatcc actggtaacg cccgctggct tttagttgct gtaagccgga gcaaatatcg
    27901 ctcagccggg tttgccagtg ctgtggattt tcttgttctt caatggataa ctgaccaaaa
    27961 ggcagcaaat cgagctgcga ggtgtagcgc aacccagcgt cgcgccagtc ctagccatcc
    28021 agcatagctc tggcccagcc ctgacggtgg gtaaaatcaa cattaaatga caggcgcaac
    28081 aagttgtccg ggcaggcatc gaccaccagg acattttctc ccaacatttg taatgaccag
    28141 gctaatgcgg cggtgatggt t
//
LOCUS       DAZFDC010000057        27370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFDC010000057 DAZFDC010000000
VERSION     DAZFDC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.057"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..1146
                     /locus_tag="ACHJ48_002509"
     CDS             130..1146
                     /locus_tag="ACHJ48_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO4708054.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTATEQGQKMNALTTDVAVQQQNERTQYDKQMQSLAQESAQAHEQIDS
                     LSQDVTQTHQQLTNTQKRVADNSQQINTLNNHFSSLKNEVDDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            1231..1653
                     /locus_tag="ACHJ48_002510"
     CDS             1231..1653
                     /locus_tag="ACHJ48_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4708055.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQAAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            1650..1853
                     /locus_tag="ACHJ48_002511"
     CDS             1650..1853
                     /locus_tag="ACHJ48_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708056.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIH"
     gene            complement(1857..2264)
                     /gene="cueR"
                     /locus_tag="ACHJ48_002512"
     CDS             complement(1857..2264)
                     /gene="cueR"
                     /locus_tag="ACHJ48_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO4708057.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(2261..3436)
                     /locus_tag="ACHJ48_002513"
     CDS             complement(2261..3436)
                     /locus_tag="ACHJ48_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFO4708058.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(3433..5595)
                     /locus_tag="ACHJ48_002514"
     CDS             complement(3433..5595)
                     /locus_tag="ACHJ48_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFO4708059.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWSLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(5599..16977)
                     /locus_tag="ACHJ48_002515"
                     /pseudo
     CDS             complement(5599..16977)
                     /locus_tag="ACHJ48_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(16974..18128)
                     /locus_tag="ACHJ48_002516"
     CDS             complement(16974..18128)
                     /locus_tag="ACHJ48_002516"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO4708060.1"
                     /translation="MTATVSDKAGNPASADHALVVDITAPDLTINTVAGDDIINAIEH
                     GQALVVSGTSTGAAAGDVVTVTLNGKNYTTTLDASGNWSVGIPAADVTALATGSQTIT
                     ASLSDRAGNSDSTTHDVTVDLSGPTLTINTVSGDDIINAAEIVVAQTISGQVTGTAVA
                     GNTVIVTIGGNQYNATVQSDLSWSVSVPANVLQALGNGELTISASLTNSANNTGTATH
                     DIVIDANLPGLRVDTVAGDDVINSIEHTQALVITGSSSGLAAGAALTVVINSVTYGAT
                     VLADGSWSVGVPVADVTNWPAGTVNIAVSGTNTAGTTTSISHPVTVDLAAVAITINTL
                     STDDVINAAEKGSDLQLSGTTSGVEAGQTITVIFGGKSYTTTVAADNTWG"
     gene            complement(18123..21335)
                     /locus_tag="ACHJ48_002517"
                     /pseudo
     CDS             complement(18123..21335)
                     /locus_tag="ACHJ48_002517"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
     gene            complement(21372..25757)
                     /locus_tag="ACHJ48_002518"
     CDS             complement(21372..25757)
                     /locus_tag="ACHJ48_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO4708061.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHITFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHAQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSDIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVD
                     VPAADIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAESTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSAWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASALASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTITLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDN"
     gene            complement(25860..27215)
                     /locus_tag="ACHJ48_002519"
     CDS             complement(25860..27215)
                     /locus_tag="ACHJ48_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFO4708062.1"
                     /translation="MARFQFKNRKNNGLIFFISFMVMGEAAIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPGVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
BASE COUNT         5117 a         8041 c         7919 g         6293 t
ORIGIN      
        1 atgcgcttta catcacacaa atggcggcgt agatttcgat taaattgcaa cgcagtttat
       61 ttcttaaaac aatattattt gtttcttata gaaacattaa tacgacttat tttgaacaag
      121 agaaaatgaa tgaaaactgt aaacgtagct ttactggcac tcataatttc agcaacatcc
      181 agccctgttg ttttagctgg tgataccatt gaagcggcgg caacagagct ttcagccatt
      241 aactctggca tgtcgcaatc ggagattgag cagaagatta cccgcttttt agaacgcaca
      301 gacaacagcc ccgctgcgta tacctatttg actgaacatc actacatccc ttctgaaaca
      361 cctgatacca ctcagactcc cactgtccag acagatcctg acgcaggaca aaaaaccgtt
      421 gccgctacag gtgatgtaca gacaactgcc cgttatcaga gcatgatcaa cgcccgacag
      481 tctgcggtaa ctgacgccca gcaaacgcaa attacagagc aacaggcgca gatcgtagcc
      541 acacaaaaaa cgctcgccgc gactggagat acgcaaaata ccgcgcatta tcaggaaatg
      601 attaatgcca gactggcggc tcaaaatgag gctaatcagc gcaccgccac tgaacaaggg
      661 cagaaaatga atgcgctgac aaccgatgtg gcagtacaac agcaaaatga aaggactcaa
      721 tacgataaac aaatgcaaag tctggcgcag gagtctgccc aggcacatga acaaattgac
      781 agcctgtcac aagacgtaac ccaaacgcac caacagttaa ccaacaccca aaaacgggtt
      841 gcagataaca gccagcaaat taacacgctc aataaccatt tcagttcgct aaaaaacgaa
      901 gttgatgaca atcgtaaaga agccaatgcg ggaactgcat ctgccatcgc tatcgcctca
      961 caaccacagg ttaaaaccgg tgacgtgatg atggtgtcag cgggagcggg aaccttcaac
     1021 ggtgaatctg cggtgtctgt cggaacatca tttaatgccg gaacgcatac ggtacttaaa
     1081 gccggtattt ctgcggatac acaatctgat ttcggcgcag gtgtcggcgt gggatattcg
     1141 ttctaatatt tcaatcctca atataaataa gagcaaggaa gcttgccggg ttcacctctt
     1201 cattaatttg tacattattt aaggttaaca atgatgaata gctccattaa atcgttttcc
     1261 ctgctggcgg ttatattact ggctggctgt agttcaccca cttcccgcat cgcagattgc
     1321 caggcgcagg gcgtcagtca tgacacctgt tacctcgcag aacagcagcg tcaggcggct
     1381 attttaagtg catccgaggc acaggcattt aaaaatgcag aagccgcaca acacgcccag
     1441 gcggcaaaga aagccattta taaaggattt ggcatgacct ttagaatgag cagtaaaaac
     1501 tttgcttatc tcaatgattc attatgtgca attgatgaag acaataaaga tgccactgtt
     1561 tatcagtcag gtctatataa cgtcattgtt tatcatcaca caggaaaagt cgccttaatg
     1621 aaagaaggcc agtttgtggg ttatttaaaa tgaaggagca aaggaaaata cccctgacgc
     1681 atattatgat tatcggtgcg tttatttttg ccttcttgca agtagtatta ttagcctccc
     1741 tggttcacgc tgtgaatgtt aacaacgaaa tccaggaagg cttatttcag tcggggcgca
     1801 ttatggtaga aagtttgcag catattcttt cggtgcaaac ggggattcac tgattttcac
     1861 cccgcccgat gatgacagca gccggagaga ttttcgataa tcgggcagtc ggcgctgtca
     1921 tcaccggggc aggcattcgc cagcgccagt agctggttgc gcatagattg cagttcttcg
     1981 atatgccgtt caatctccgc caccttctcc agcgtgcgac gtttgacgtc ggcactgtga
     2041 cgctgcgggt cgttaaacag attcaccagc tcgccgctct cttccaggtt aaagcccacc
     2101 tggcgcgcct ggcgcagtaa ggtcaattcg ttgagatgct gctgcgtgta ggttcgataa
     2161 ccattttcgc tgcgcatcgg cggcgtcacc agccccttct cttcatagaa gcgaatggct
     2221 ttgctggtca ggccggtaat ttttgctaca tcgctaatgt tcatcgttcg cgcaacgcct
     2281 cctgtgcttt gttaaagggt ttcaacagat aatccagcac gctttttttg ccggtgcgga
     2341 tatcgaccgt cgccaccata ccggggaata tcggaaactg ctgtttgctt ttgttttcca
     2401 gatggttgct gaaagtgcga atgtacaccc gatagtaata aacatcgcgc cgcacttcat
     2461 cctgcacggt gtctggtgat atcaccgcca cttctcccgg caggccgccg tagatggagt
     2521 aatcgtaggc agttattttc accagcgatt tttgccccgg gtggataaac gccacatcgc
     2581 gcggggagat ttttgcctca atcagcagtt gctcatccag cggcacgatg gtcatcagtt
     2641 taccaccggg ggcgatgacg ccgccaaccg tggtgacatc aatatcctga acaatgcctc
     2701 gcaccggcgc ggtaaagttg agcctggtga gagagtcttc ccgtccacgg atcaccgaac
     2761 gctgggtttc cacttccgca ttggcttttg ctaactcttc gcgcgcctga acgtagtatt
     2821 gcgtgcgcac gtcgctgagt ttgttttcca gttcattggc ctggcgttgc aggcgcagta
     2881 cttcgacgct gctcgctgcc ccctgtttta ccagcggctg ggtcatcgcc agttcgcgat
     2941 tgaccaggct aatcccctgc gtcagcccgg tggtggcttt gttcaacccg tcgcggcgag
     3001 tgttgtacaa cgccgtttcc gactcgataa gttcggggaa tttattcagt tcgtcaggga
     3061 agaccagcgg tttattgctc acttccgccc gcagccgcgc cgaggttgcc agcgccgcgt
     3121 acagccgcga catcgcttcc tggacgtcgg attccgtttt ggtgcggtta agctgcgcca
     3181 gcggctggcc ctgttcgaca atatcgccaa ctttcacgtc gagatgatac agaattccgc
     3241 cttccagcga ctgcaccacc tgttcgcgcc cggaaggttc taccgtgccg ctgccggtgg
     3301 tgacttcatc aagctggaag taccacgccc agccgagaaa acagagcagc atcacagcgg
     3361 taacgcgtac cgccagcgcc gaacgcggca ggcggggttc agtcagacca cggttatatt
     3421 gcagttgtcc gttcatgccg cagacccctt attcttctcc tgcaacacca cccgtcgcgc
     3481 tgtgggctcg ccctgttcac gcaaaatggc ctctttcgcg ccgtccatca caatccgccc
     3541 ctgatccatc acgattatcc tatccaccag ttgcagcatc gccgtgcggt gagtggcgat
     3601 aatcagcgtg cgatgcccaa tccactgttt cagttgttcg attaaatacg cttctgccat
     3661 ttcgtccagt gaggcgctgg gttcgtcgag caggacgata ttcggctgag agatgagtag
     3721 ccgcgccagc agcagcgttt gccgctgacc accagagagg ccaaatcccc cttcctgaat
     3781 catgtagttg aggccgtttt tctgcttctg cacaaagggc agcgctccgc tgagggttaa
     3841 cgcgcgttcg atatcttcat cgctggcttc cggcatcccc atggtgaggt tttcccgcag
     3901 cgaaccaaag aacagtcgcc cggtctggct gagtagcccc atgtctcgcc gcagatctgc
     3961 cggatcaagc tgcccgatac tgatgttatc cagcaacacc tgcccctgct gggcgatacg
     4021 cattccggcg agcatttgca gtagcgtcga tttacccgcg ccgttgcgcc cgagaatggc
     4081 gattttctca ccagcctgaa tgttgagttt gccaatcgct acatcggcaa tcttctcttc
     4141 ttcgtcgtaa taaaacaccg cgttgctgaa ctgataatta ccgtgcagca ccgctttatg
     4201 caccagtttg ccgtgttccg gctgatccac cgggcgcttc atcagttcat caaggccgtt
     4261 acgtgctact ttcgcctgct gccagcgcga gagcacgccg gatatctgcg acagcggcgc
     4321 aatggtgcgc gaagccagga tcgtcgtccc gaccagcgcg ccggtggtca tgtcgccgtt
     4381 catcaccata aagcagccca ccagcagcac caccacgtag acgatggact gcacttcctg
     4441 ggtccaggtg agcagcagcc cggtgagaaa acgttgcttc atgctgatgg acgaggccac
     4501 gtcgttggtg tggttccact gattctgaaa acgctgttcg gcgcgcagca gtttaatatc
     4561 ttcaatcgac tgcacggctt ccaccagcgt agcgttgcgt accgctgact cgcgcattcc
     4621 ttcgttcgcc agccgcgcca gcgggcgttg caccagcagg ccggggatca ccagcaacgg
     4681 cagcgccagc aaaaccacca gcaccagcca gccgccgatc atccacaaaa taaagacaaa
     4741 cagcaggaag aatggcaggt ccgccaccgc gccgatggtg gtggacgtaa taagctcccg
     4801 cactgattcc agttcgcgga tctgcgagat aaacgatccg gtggatttcg atcgcacgtt
     4861 gtttttcagc cgtagcgcat gaccaaaaac gcgatccgaa atacgcaggt ctgcccgctt
     4921 tccgatcaca tcagatagat gcgtacgcac catgcgcata cagaactcaa acaggatcgc
     4981 catcatcacc ccggcgaaaa gcacccatag cgtcgggtac gattgcgccg gaaccacccg
     5041 atcgtaaacc tgcatcgaga aaatcatcgc cgccagcgcc agcacgttcg ccaccagtga
     5101 cgccagcata atatcgccgt agcgccgcca gtcttttagc gaaagactcc agaaccagtt
     5161 cgcctgatac gggcgaatat agtcatccac ccgcgcgtca gggatggcgc tgagagggcg
     5221 cagcagcgcc agttcgacga ttttatcttc aatatccgcg acgttaagtg tagtttccag
     5281 cccttcgtca ccgctgaact gaacgctgat attgtcatga ttgtcgcgac gggtgatcac
     5341 gccaatctgc tgattatcaa acaccgcgat cagcggcaga cgccaggggt taagcgaatc
     5401 ggcggaaaac tccaccaacc gcaaccccat ccctaactgc cgcgccatgt cggtgagtaa
     5461 ctcctcccgc ttactgtcgc gctcccagtt gacggtcgcg cgcacattct cctgagaaaa
     5521 atcgagccga taatagcgcg ccacggcgat catcgcctcc agccaggctt ccacggactg
     5581 ggcatttttt ttcattatct acaccaccat caactgatga ttcgccagca aggtggcgag
     5641 gtcggtgtgc acgcccgtca gcgtgaccac gttagttgtc gcaaaagtgc cgcccgtgcc
     5701 gtcgcggtcg atctgcacga tggtgtcgct gccgctctgg gtgactttga caaagtcacc
     5761 gatgtttccg gcctgcgcat ccagcgttgc cacgccgttg acgtagctgg ctttgccgtt
     5821 gccggtgtag ccgctgccct gtaacagttc acgaatatca atgcgatcgg tgtcggcggt
     5881 gccttcccat gtccctaccg taaagccatt caccacgtct gagccgttgc cgccagtggc
     5941 gtcgctggcg ttgagcagtt tatagagcaa ggtgtcgtgg ccgccgttgc cgatgttgag
     6001 cgtgtcgttg ccgccgcgcc cttccagttg gttatcgccg ctgctgccgg tcagaatgtc
     6061 gttaaaattc gagccggaaa tgccttcgat attggtaaat gtcgaggtgt taaaaccggt
     6121 ggcctgcgcg gcagtgctgc ttaagttagc cgtcacgccc accgtcgaaa gccggaaatc
     6181 aaccacgtcc atcccgccgt tcgccagcca cgttccggtg ccggagctgt acacccagcc
     6241 gccggaaccg tcgtaagtgt cggtgcctgc ggtggcgaag aaggtgtcgt tatagccggt
     6301 gccgacgaac ttgccgttgt tgctcgcggt acccgcttca gcgctgagat cgtaagcgtg
     6361 agtggcatcg gtggcctgta aaccgcccca gctggtgttg accgtctggt cgatattgct
     6421 ggcggtggtg ccggtggatt tgatcagcac cgcgttgtag gtttctccgg cattcagccc
     6481 gtagaagttg accagcgccg aggtgtcgtt aacccccata cccgactgcg ggttgatgat
     6541 ttgcgtggcg accagcactc ccgccgagtt gtacagcttc acggtattgc cgtaatagac
     6601 gttaatgccg ttgggatcgg tgatgcgcag gtgtagcgaa gtgccgtagc tcaccgtgtt
     6661 ggtgttgcgg ctcaggaaca cgctgcccga ctgtttggac accagcacat ccaccgcgcc
     6721 gtcccagtcg tagtccattg ccgccacgcc gctggtggta ccgctctggc tgccgccgag
     6781 ggccgattgc gtccagttgc tggcgttgct gacgttagta taaagatacg actgcccggt
     6841 ctgggcgata cgggcgatat ccatcaggcc gtcgtggttc cagtccaccg ccaggctaaa
     6901 ctggctggcg taggtggttg ccgttgcgcc caccgccacc gggctaccca gtacgccgtt
     6961 ggtgttgaat aacagcgagc cgtagttagc tcttccctgg ctggcgggca ggaagagatc
     7021 catatcgccg tcaccatcga aatcggccca ggtcatggaa acgctgctgc tcatagcgcc
     7081 actgcccgcc ccgctcagga aggtattggt ggtgttctgc ccccagacaa acgtcccatt
     7141 cccctggttg atgagcgaag acagcgtgta atagttgttc aggttatagg tgtgctgaac
     7201 caggtcgatc ctgccgtcat tgttcagatc gacgccagaa ccttcgttga gcgaaagata
     7261 ccccgtcacc gccccgccca ccgtggcgct accgccgctg ttcgacgtgg tggagttgcc
     7321 taccagcgtg cctgcgttgt tccacaggaa ggtgttggag tccggcccgc cagcgtcacc
     7381 aatccagaag tcgagatagc cgtcgccctc tttatcaaat gccacaatcg agccgtacca
     7441 cgtcagggtg ccgacggtga cctgaatagc gctggaaaag gtgccatcgc cgttattggt
     7501 aaagccgtta atatagcccg taccgtagtc atcacgggtg ataaacagat cgcccgtgcc
     7561 gttacggtcg taatcggcaa tggagctggt ggcgtagttg ttgccgctct gatacagcgt
     7621 cagcgcggtc ttcgaccagg tgagtgggtc agtcgattgc attacctgct ggtttgccag
     7681 caccgtccac atcccgtgcg agtcgaggcc gtaggtcagc ccccaggcgg tggttttgct
     7741 ggcggtagtc caggtcggtg tgtaatcaat cgccgcgtta accgtcaccg tgccgttgct
     7801 aatattggcg ttattgccgt tacccgccga actttttacc tgcgcagtaa cggtataggc
     7861 ggtcgcggaa actgtcagcg catcggtatc cggcaactgt acataccagg tgttgtgcgc
     7921 cggatcgacc actaccgcgc cgcccggttc agaggtgtag gttttgccgt tgatcaccac
     7981 ttcaacatac tgcccactgg caagcgcggc ggtgatcacc ccgctaataa tcggcgtagt
     8041 atcgcgcgtg gtctggctgg tgatttgcgc cagcgtggtc ggaatagagg tatcgaccgt
     8101 caggacaaaa tcactggagg aggtgatatt ccccgccaaa tccaccactc gcgcgctgta
     8161 ggtataggca ccgctcacca gcccgctgtc gctgtaggtc cagttcagag cgccgaccat
     8221 cgtcaccgcc cctaacagca gcccgttacg gtagaggtaa accacttcac cgctggcaag
     8281 cggcgcggaa agtacaccgt tcagcagcgg cgtagtgtcg tcggtggcct gcgaactgct
     8341 taatgtcccc tgccgctgcc cgacatcatc ggtatacgac acaatcgttg gtgtggcggt
     8401 ggggttaatc gtatcgacca ccaccgtttt cgacaccacc gggccggtgt tccccgccag
     8461 atccagcacc cgcacctgat aaacgtagct gccgtcggtc agcgtgcggc tgtcggtgta
     8521 agtccactgt gtaccgttgg tggtgagcgt tgtccaggtc gcgccgctat caaggctaat
     8581 ctgcgccact tcgccgctgg caagccccgc ccccagcgaa ccggtcagcg tgagcatggt
     8641 gtcgctggtg ataaagtcag ttgccgaact ccccgtgtcg gtgctgatgc tggcgatggt
     8701 gagcacctgc gccgggttaa cggtatcgat cagcgccgac tggctgctcg ttgcgccaac
     8761 gttgcccgcc agatcgacca cccgcacctg ccaggtggtg gtgccgttgg tgagggttcg
     8821 tccgtcaacg taactccagt tcaggctgtc tgcggcgacg gtcacgttca cccaggttgc
     8881 gccgttgtcg gtactgattt gcgcgaactc attagcgccg agcgccgcgc ccaatacgcc
     8941 gcgcacggta agcgtggtgt cgctggtaat aaaatcgctg ctgctggttc cggtgtcatc
     9001 gctgataccg gtaatggtga tacttttcgc cgcttccggg ctggtggtgt cgaccaccac
     9061 gttttgcgtt gcggtctgcc caacgttccc cgccagatcc accacccgca ccgtgtagtt
     9121 ccaggtgccg tcagtaagcg tgtgaccatc ggcatagctc cagctggtgc caacgacggt
     9181 taacgtagtc caggtgacgc cgccgtcgag gctgatttgc gcgaactcac cggcagaaag
     9241 cgtcgccccc agcgtgccgc tcaccgcaag cgtcgtgtcg ctggtgacaa aatcgttagt
     9301 gatcaagccc atatcggtgg tgatcgcgct gatggcgatg gttttcaccg cgggatctgg
     9361 cgcggtagtg tcgatcacca cattctggct gtcggtcgcg ccaacgttgc ccgctgcgtc
     9421 aatcacccgc acctgataga gatagttgcc atcggtcagc gtgcggctgt cgttatagcg
     9481 ccaggtcgtg ccggtaacgg taagcgtggt ccaggttgtg ccgccgtcga tacttatttg
     9541 cgccgtctcg ttactggcaa gcgcggcggt gagcgagccg ttgaccacta ccggactgac
     9601 gctggtgata aagtcgctgg cgctaaggcc agtgtcggct tgcagggaat cgatattaat
     9661 gcccatcgcc ggagcgctga gatcgacggt aatggtgtga ttactgctac cggtgttgcc
     9721 aatggcattg ctcaccgagg cgttaatcac gtaggcgttg ccgtccgcca gcgcggtgac
     9781 atcggcgctg ccgagggtgt agctccagct accgcccgtc tgcaccgtgg tggtgtaggt
     9841 tttgccgttt aaggtcaggg tcagcgtctg cccgacgggc gcggtggtgg tgccgctgat
     9901 caccagcgat gagccgtgtt ccgctgcatt caccacatcg tcgcccgcaa aggtgttgat
     9961 ggtgacagta ggtacatcgc cgttcagcgt cacgccacgg ctggcgctgc cggggtttcc
    10021 ggcctgatcg ctaaccgtcg cgctaatggt gtagctgccg tcgcttaatc cggcaaactg
    10081 ctgcgccgga ataaacaccg accagcttcc gccgctgcca accgttgcgc tccaggtttg
    10141 cccgttaaag gtaacggtca ccgtctggcc cacttcggcg gtggtggtgc cgctaatggt
    10201 ctgcccggca agctgctcgg cgttgttgat aatatcgtca cccgccaccg tggcgatggt
    10261 gaccgttggc gcaacggtat cgacggtcag ggcgtgcgtg gcctgtccgg ggttaccggc
    10321 gcgatcgttc acgctggcgg taatggtctg cgcgccctgc ccgagcgcgg ccagatccac
    10381 cgcaggcacg ttcagcgccc aggtaccatt ggcggcaacc gtggcggtat aggttttgcc
    10441 gccaaaggtg acggtgaccg tctgccccac ttccgcgctg gtcgagccgt taagggttaa
    10501 cggctgctgc tgttcggcgg cgttgaggcg gtcgtccgtc gaaacggtgt taatgctgat
    10561 aaccggcgcg gtgagatcca ccgccacgct gcgcgaggcc gtggcgctgt ttccggcact
    10621 gtcctgagcg gaggcgctaa ccgtgtagct ggtgccatca ctgagtgccg caacatccgc
    10681 cgcaggcacg ttcacgctcc agctcccgtt gctctgaatg gtggcgctgt agcctttgcc
    10741 gttaagcaac acggtcacca ctgtacctgt cgcgaaatta gtgccagtgc cgctcagggt
    10801 cagcgcactg cccgcttcgg cggcgttaat aaggttatcg ccgctgatag tgctgaccga
    10861 gagcgacact gccgccgtgt tcacctgtac gttgtgcgtc tgcgtgctgc tgttgcctgc
    10921 gctatcggta atggtggcgc tgatggtcac cgtgccatcc gcaaggccgg aaatcacgct
    10981 ggcaggcacg ccgacgctcc agttgccgct ggcatcggtg ctggtgacat actgctgccc
    11041 ggcgatggtc accaccaggc ggtcgcccgc caccgcgccc gtggcggtgc cgctgataat
    11101 ctgcgcctga atgtgttcga cgttgttaat cacatcatcg cccgccacgg tgttaaaact
    11161 gatcaccggc gcagtggtat cgacggtcac gcctttgctg gcgctgccaa ggttacccgc
    11221 cagatcgctg accgtggcgg tcagggtgta gccgttatta gcaagtgcgg ttaagtcgct
    11281 ggcgggcagc gttaaactcc agctgccgtc tgtctgcacc gtggtctggt agttagtgcc
    11341 gttaagcgtg acggtgagcg tctgcccggt ttgcgcggtg ctggtgccgg acaccgttac
    11401 gcccgccgcc gcttcgctgg cgttgatgat gttatcgctg gcaatggtgt tgatggtcac
    11461 cgttggcgcg gcgctatcga cgctgtattc gtgcgttgct gtggcgctgt tgccgttgac
    11521 gttagtcacc cgcacctggg cgctggcatc gccatcacgc agggccgcca aatccgccgc
    11581 cggaacggtg gtgctccagc taccgtttgc cgccaccgtg gtggtgtagg ttttgccgcc
    11641 aaaggtgacg gtgacggttt gccccgcttc gacgccggac gtgctgccgg agagcgttaa
    11701 cgccgcgcct ttttcggcgg cgttaatcgc atcatcgctg gtgatgctgt tcatgctgat
    11761 ggcgacggcg gtgagatcaa cagtcagcgg atgggtaatg ctggtttgcg ttccggcact
    11821 gttcgccccg ctaacggtaa tattcagcgt tcccgcaggc cagttactga catccgtcgc
    11881 aggaacgccg acgctccagc taccgttcgc caacaccgtg gcggcgtaag tttggttgtt
    11941 aatcaccacc gtcaacgccg tgcctgcggc aaagccggag ctgctgccgg tgatcacctg
    12001 cgcctgagcg tgttcgatga tattgaccac atcatcaccc gcgacggtat ccacccgcag
    12061 gccaggcaga ctggcatcga tggtgatatc gcgcgtgccg ctaccggtgt tgccaacggc
    12121 gttggtcacc gaggcattaa ccgttaactc accgttaccc agagcctgcc agtcggcggc
    12181 aggtacgctc acattccagc ttaaatcagc ctggacggtg gcggtgtaag ttttaccgcc
    12241 cagttcaacg gtgacggtgc tgcccgccgc cgcaccggtt acctggccgc tgatagtttg
    12301 cgcgctgcct gcctctgaag cgttgataat atcgtccgtc gccaccggat taatggtgat
    12361 gcccggcagc gcggtattaa cctgcacgtt atggctggcg gtgctgctgt taccctgagc
    12421 attggtgacg ctcgccgtca cgctgtaggt cgcttcgccc agcgcactga ccgccgaggc
    12481 aggcacggta acactccagt tgccggaggc atccgtggtg gcgctgtagt tttgtccgtt
    12541 cagggtgacg gtgatcgcca tacccgcagg ctgatcgctg gtgccagaaa gcgccagatc
    12601 agatcctttt tccgtcgcgt tgatcacatc atcgcccgcg atggtgttga tgctaatcac
    12661 tggcgcgctg aggctgacgg tcacggtgcg gctggcgtcg tcgctgttgc ctgcccgatc
    12721 gctgacgctg gcggtgatgg tctgcgcacc gctacccagc gccgtaacat ctgccgccgg
    12781 aacgccaacg ctccagttgc cggaggcatc cagggtggtg gtgtaggttt tgccgttgag
    12841 cacaacgctc accacatcgc ccgcttcccc gccgctactg gtgccggaga tcaccagcgc
    12901 ctgcgcatgt tcggcatcgt tgatgatgtc atcgcccgcc acggtgttaa tggtgagaac
    12961 cggcgcggtg gtgtctaccg ccagattatg gctggcgctg ccggggttgc ccgctttgtc
    13021 attgaccgtg gcgctgacgg tgtaattgct tgcgttgagc gcgcccaggg ctgaagtggg
    13081 aacgctgacg ctccagctac cgtccgcctg cacagtgccg gtgtaggttg cgccgttaag
    13141 ggtgacggtc accgtctgcc cggtttcggc ggtgctggtg ccgctgatgg tgagcgggct
    13201 tccggcttct gcggcgttaa ggatatcgtc gctggcgatg gtgttaatgg tgagcgtcgg
    13261 cgcgctggcg tcaacgctat acgcatgggt cgccgaggca ctgttaccgc tcgcgctgct
    13321 aacgctggcc tgcacattcg ccgcgccctc tggcagagtt gccagatctg ccgccggaac
    13381 gtttacactc cagctcccgt tggcggcgac gcttgcagtg taggttttac cgccaaaagt
    13441 gacggtgacg gtttgcccgc tctcaatccc gctggtactg ccggaaagag tcagattggt
    13501 gcctttttct gcggcgttaa tcacgtcgtc gctggcaacg gtgttgatgc tgattgccac
    13561 cgccgttaaa tcgacggtga acggatggct gacgctgact gggttattgg cgctgctttg
    13621 cccgtctacc tccacggtta acggccccgc aggccaggcg ctgacgtttg ccgccggaat
    13681 gccaacgctc cagcttccgt ccgcctgcac cgtggcggaa tacgccacac tgttgatggt
    13741 aaccgtcagc acagcacctg cattcaggcc gctactgccg ccggtgatca ccagcgcctg
    13801 cccgtgctcg atgctgttga cgatatcatc gcccgccacg gtatctacgc gcagccccgg
    13861 caggttggca tcaatggtga tatcccgcgt gccgctcccg gtgttgccat tagcgttggt
    13921 gaccgaggcg gtaatggtca aatcaccatt gcccaacgcc tggagatcgg ctgtcggaac
    13981 gctgacgctc cagcttaagt tgctctgtac cgtagcggtg taagtgttcc cgcccagcgt
    14041 cacggtgacc gtatcgccag cggcggcgcg agtcaccacc ccgctgatgg tttgatcggc
    14101 ccccgcttcg gcggcgttga taatatcgtc gcctgccacg gtgttgatgg tgacgtccgg
    14161 caggccgctg tcgaccagta aattcgcctg gctgctggcg gtgttgcctg ctgcactggt
    14221 ggcgctggcg ctgaccgtat agttggcctg acctaatgcc gccagatcgc tcaccggcac
    14281 ggttaaggtc cagttgcccg atgcgtcggt ggtggcgctg tagttgtgac cgttgagcgt
    14341 gacggtgacc tgcgcgcccg tcgccagccc cgtactgccg ccgctgatgg tcaacggctg
    14401 gcctttttca tcggcgttca ggatgttatc gccgctgatg gcgttaaagg taatggtcgg
    14461 cagcccggta ttgaccgtca cctgatgggt agcgctgccg ctgtttcctc cggcatcggt
    14521 gacgctggca ttgatggtca ccgtgccatt cgccagtgcc gagacgacgc ttgccggaac
    14581 gccgacgctc cagttgccgc tggcatccag caccgtggta aaggtatttg tgccgatagt
    14641 caccgtcacg gtgctaccgg ttgccgctcc agtggcggag ccgctgatga tctgcgcctg
    14701 ggcgtgttcc gtcgcgttga tcacatcatc gcctgccacc gtgttgatgg tgacgaccgg
    14761 aacggacgta tccaccgtca ggttgtgatt aaccgaggcc gggtttccgg ctttgtcgct
    14821 caccgccgcg ttcacggtgt agttgctggc ggtcagggtg cttaagtcgg ctgacggtac
    14881 gctgacgctc cagctgccgt ccgtctgtac ggtgccggtg tagtttgtgc cattgagcgt
    14941 gacggtcacc gtctgccccg cttccgccgt gctgctgccg gtgatggtca gagccgctcc
    15001 ggcttcggcg gcgttaagaa tatcatcgcc cgcaatggta ttaatggtca ccgttggcgc
    15061 gctggcatca acgctgtaag cgtgggtcgt ggtggcgctg ttgccgttaa cattgctgac
    15121 gctggcctgt gcgctggcat cgccatcacg cagagccgcc atatctgccg ccggaaccga
    15181 ggtgctccag gaaccattcg cagccaccgt ggcgctgtaa gttttgccgc caaaggtgac
    15241 ggtaaccgtt tgtccggctt caacgccaga ggtgctgccg gagagcgtta acgccgcgcc
    15301 tttttcggca gcgttgatca catcatcggc ggtaatggcg ttgatgctca ccgccaccgc
    15361 cgagagatcg accgtcaccg gatgcgtaac gcttaccgga tttccggcag aggtgctacc
    15421 gctcgccgca atcgtcaccg atcccgcagg ccaggcgctg acatccaccg ccggaacgcc
    15481 gacgctccag gtgccatccg ccagcaccgc cgcaacatag gtttgcccgt taatggtcag
    15541 cgtgacgttg ctgcccgccg ccagaccgga gctgctgccg gtaatcacca gcgcctgccc
    15601 gtgctcgata atattaacca catcatcgcc cgccacggtg tcgacccgca gaccggggag
    15661 attcgcgtct atggtgattt cgcgcgtgcc gttaccagta ttgcctacgc tgttcgtcac
    15721 cgaagccgaa atggtcagtt cgccgttgcc caactcctgt agcgcggagg ccggaacatc
    15781 gacgctccag cttaaattcg cctgcaccgt ggcagtgtaa gtcgccccgc cgagcgtgac
    15841 ggtcaccgtg tcgcctgccg ccgcacccgt cacctgcccg ctgatggtct gttccacgcc
    15901 cgcttcggcg gcgttaataa tatcgtccgt tgccaccacg ttaatggtga cgcccggcag
    15961 cgcggtatta acctgtacgt tatggctggc ggaaccgctg ttgccatcgg cgtcagtggc
    16021 ggctgcggtc accgtgtagg tggcttcacc gagggcgctc acccgtgacg ccggaacggt
    16081 cacgctccag ttaccggaag catcggcagt ggtggtgtaa ttttgcccgt tgagcgtaac
    16141 ggtaatctgc gtgcccgcag gctggttgct ggtgccggta atcgccagat ccgcgccttt
    16201 ctccgcggcg ttgatgatgt catcgacggc aatggtgtta atggcgagca ccggagcgcc
    16261 gagcgccacg gtgacagtgt gcgaggccgt gccgctgttg cctgcggagt cggtgaccgt
    16321 agcggtaatg gtcacatcgc cctgcgccag cgcggaaatc acgctggcag gcacgccgat
    16381 gctccagttg ccgttggcgt ccagcacggt ggtataggtg gtcgtgccaa tcgtcacgga
    16441 aaccgtatta ccggtagtcg cgcccgttgc cgagccgctg atgatctgcg cctgtccgtg
    16501 ttccgtggcg ttaatgatgt catcgcccgc caccgtgttg atggtgacga ccggagcggc
    16561 aaggtccacc gtcagattat gcgttgccga agccgaattt ctggctttgt cgctgaccga
    16621 ggcgttaacg gtatacgagc tggcggtaag gctagctaaa tcgccggttg gtacgctgac
    16681 gctccagctc ccgtctgcct gcacattgcc gctgtaatta acgccgttta gcgttacggt
    16741 caccgtctgc ccggcttccg ccgtgctggt gccgctgatg gtcagcgccg atcccgcttc
    16801 ggcggcgtta agaatatcgt ccgtggcgat ggtgttgatg gtaacggagg gcgcagtggc
    16861 atcaacgctg taagcatgcg ttgcctgggt gctgttccct gcgacattgc tgacgctggc
    16921 ctggacgttt gctgcgccgt ctggcaaggt tgcaacatcg acggcaggga tcgtcagccc
    16981 caggtattat ccgcggcaac cgtggtggtg tagcttttgc cgccgaagat aacggtgatg
    17041 gtttgccccg cttccacgcc ggaggtggtg ccggagagct gcaaatcaga gcctttctcg
    17101 gcggcgttaa tcacatcgtc agtggaaagg gtgttgatgg tgatcgccac ggcagcgaga
    17161 tcgaccgtga ccggatggct aatgctggtt gtggttccgg cggtgttggt gccagagacg
    17221 gcaatattga ccgtccccgc aggccagttt gtgacatctg ccaccggaac accaacgctc
    17281 cagctaccat ccgctaaaac cgttgcgccg taggtgacgc tattgataac caccgtcaac
    17341 gccgcgcccg ccgccagtcc gctactgcta ccggtgatca ccaacgcctg agtgtgctcg
    17401 atgctgttaa tcacatcatc gcctgccacg gtatcgacgc gcagacctgg caggttggca
    17461 tctatcacga tatcgtgcgt cgcggtgccg gtattatttg cggaattggt caacgaggcg
    17521 ctgatggtca gttcaccgtt acccagcgcc tgcaaaacgt tcgccggtac gctgacgctc
    17581 cagcttaaat ctgactgcac ggtggcgtta tattgattgc cgccaatggt gacaatcacc
    17641 gtattcccgg caaccgccgt tcccgtgacc tgaccgctga tggtctgcgc cacaacgatt
    17701 tcagcggcgt tgataatgtc atcgccggag acggtgttaa tggtcagcgt cgggccgcta
    17761 agatcaacgg tcacatcgtg agtcgtgctg tcgctgttgc ccgcgcgatc gctcaggctg
    17821 gcggtgatgg tctggctacc ggtcgccagc gccgtgacat ccgccgccgg aatgcccacg
    17881 ctccagttac cggaggcatc cagcgtggtg gtgtagtttt taccgttcag cgtgacggtt
    17941 accacatcac ccgccgccgc gcccgtactg gtgccgctga ccaccaacgc ctgaccatgt
    18001 tcgatggcgt tgataatgtc atcgcccgcg acggtgttaa tggtgagatc cggcgcagtg
    18061 atatctacca ccagcgcgtg gtcggcgctt gccgggttac ccgctttgtc gctcaccgtg
    18121 gcggtcacgg tgtaactgct ggcggttaat ccgcttaaat ccgctgccgg aacattcacg
    18181 ctccaggtgc catccgccag aacggtggtc tggtacgtgt tgttattgag cgtgacggtg
    18241 agcgtctgcc cggcctgcgc cgtggtagtg ccgctgatgg tgatccccgc gccagcttcg
    18301 ctcacgttaa ggatatcgtc gctggcgatg gtgttgatgg tcacaagcgg cgcggtggca
    18361 tcaacgctat acgcgcgatc ggcctgggcg ctgttgccgt taatattact gacactcgcc
    18421 tgcacgttgc ccgcgccgtc cggtagcgca gcgagatcgg caggcggcac attcaccgtc
    18481 cagctaccgt tagcttccac tgtggtggtg taatttttgc cgccaaaggt gacggtcacc
    18541 gtctgcccgg cttcgacacc gctggtgctg ccgcttacga ccagcgtttc gcctttttcg
    18601 acggcgttga tcacatcatc accagagaga gtgttaatgg tgatggcgac cccagccaga
    18661 tcgacggtaa ccggatgggt aatggtggag gttgttccgg cactgttggt gccgcttacc
    18721 gtgatattca ccgtacccgc tggccagttg ctgacgtcta ccgccggaac accgaggctc
    18781 cacgtgccgt cggctaatac cgtcgcgcca taagtaacgt tgttaatctc gaccgttaac
    18841 gccgttcccg ccgttaatcc gctgctgctg ccggtgatca ccagcgcctg attgtgctca
    18901 atgctgttaa taacatcatc gcccgccacg gtatcgaccc gcaggccagg cagattggcg
    18961 tcgatcgtga tatcgcgcga gccgctgccg gtgttgccga cgccattggt gaccgaggca
    19021 ttaaccgtca ggtcgccgtt gcccagcgcc tgaatatccg ccgccggaac gtccacactc
    19081 cagcttaaat tagactgcac cgtggcggtg taggtgttgc cgcccagcgt aacggtaacc
    19141 gtatccccct gcgctgcgcc cgtcacctgc ccgctgatgg tttgcgcatt tcccgattcg
    19201 gcagcgttaa taatatcgtc agtcgccacc gcgttaatgg taacggcagg caaggcgctg
    19261 ttgaccagca cattatgact ggcggaatta ctgttgcctg ccgtatcagt gacgtttgcc
    19321 gttaccgtgt agttggcttc acccaatgcg ctaaccgctg acgctggcac cgtggcgctc
    19381 cagttgccgt tgctatcggt agtagcggtg taattttgcc cgttcagcgt caccgtaatg
    19441 gtggtgcccg caggctgatt actggtgccg gtgatttgca ggtccgcgcc tttttccgtg
    19501 gctttaatca cgtcatcggt ggcgatggtg ttgataccaa tggttggcgc ggcgagattc
    19561 acggtgaccg tgcggctggc gtcatcgctg ttgcctgccg cgtcggtaat tgccgcagtg
    19621 atagtttgcg ggccgctgcc aagcgcagtg acgtcagccg ccggaacgcc gacgctccag
    19681 ttgccggaag cgtccagcat ggtggtgtag gttttactgt ttagtgtgac ggtgatgaca
    19741 tcacccgctt cgccgccagt gctggagccg gagatcacca gcgcctgtcc gtgttcggcg
    19801 gcgttaataa tgtcatcgcc ggagacggtg ttgatggtca gcaccggcac ggtgagatcc
    19861 accgccagcc cgtggttagc ggacgccggg ttacccgctt tatcgcttac cgaggcacta
    19921 acggtgtact ggctggcggt cagattgctg agatccgccg tcggtaagct gacgctccag
    19981 ctgccgtccg cctggacgga gccgctgtaa gtcacaccat taagcgtgac ggttaccgtc
    20041 tgccccgctt cggcggtgct gctaccgctg atggttaacg gattgcccgc ctcggcagcg
    20101 ttcagaatat cgtcggtggc gatggtgtta atggcaagcg tcggggccgt ggcatcgacg
    20161 ctgtaggcgt gggttgccga agccgtgttg ccgttaatag tgctgacgct ggcctgcacg
    20221 gtggcgtcgc cgtcgcgtaa cacgctgaga tcggcggcgg gtacggtggt tgtccagcta
    20281 ccatcgcccg ctacggtagc ggtgtaggtt ttcccgccaa aggtaacggt gaccgtttgc
    20341 cccacttcca cgccggaggt gctgccggaa agggttaaat ctgccccttt ttcggcggcg
    20401 ttaatcacat cgtcgccgga aacggtgtta atggaaatcg ccaccgccgc cagatcgacg
    20461 gtgaccggat gggtgatggt ggatgttgtt ccggctgtgt tagtgccact taccgtgata
    20521 ttcaccgtac ccgcaggcca gttactcacg tctgccgccg gaacaccaac gctccatgtg
    20581 ccatcggcta atactgttgc agcgtaagtg accgtgttaa tcaccaccgt cagcgccgcg
    20641 cccgccgcca gcccgctgct gctaccagtt atcaccagcg cctgagcgtg ctcgatgcta
    20701 ttgaccacat catcgcccgc cacggtgtca acgcgcagac ctggcaggtt ggcgtcgata
    20761 gtaatatctc gcgaaccgct gccagtattg ccaacgccgt tggtcaccga agcgttaacc
    20821 gtcagattgc cattgccgat ggcctgaata tccgccgccg gaacgtccac gctccagctt
    20881 aaattcccct gcacggtagc ggtgtaagtt ttcccgccaa gcgttacggt aaccgtatcg
    20941 cccgccgccg cccccgtcac ctggccgctg atggtttgcg cattgcccga ttcggcggcg
    21001 ttaataatat cgtcggtcgc aactgggtta atggtgacgc caggcagcgc ggtattgacc
    21061 tgcacgttat ggctggcgga attgctgttg cctgcgctgt cggtgacgtt cgccgtcacc
    21121 gtgtagctgg cttcgcccag cgcacctact gcggaagctg gcaccgtagt gctccagttg
    21181 cctgcagcgt ccgtcgtcgc ggtgtaattt tgcccgttaa gcgccagcgt aatcgtggtg
    21241 cctgctggct gattactggt gccggaaatt tgcagatccg cgcctttttc gctggcgtta
    21301 attatgtcat cggtggcgat ggtgttgatg tcaatggttg gtgcggtgag gttaacggtg
    21361 accgtgtgtg tctaattgtc gctgttgcct gccgcatcgg taaccgtggc ggtgacagtt
    21421 tgcgggccgc tgccaagcgc cgtgacatcc gccgccggaa cgccgacgct ccagttgccg
    21481 gaggcgtcca gggtggtggt gtaggtttta ctgtttagcg tgacggtgac gacatccccc
    21541 gcttctccgc cggtgctgga accggaaatc accagcgcct gcccgtgttc ggtggcgtta
    21601 ataatgtcat cgcccgcaat ggtgttgatg gtcagcaccg gaacggtgag atccaccgcc
    21661 agattatgcg tggtgctcgc cggattgccc gctttgtcat tgaccgtggc ggtcagggtg
    21721 tggctgccat cggtcaatgc ctcaagatct gccgccggaa tattgatgct ccagctgccg
    21781 tccgcctgca ccgttgcctg ataggtctgc acggtcgggc tgttaagcgt tatcgtaaca
    21841 atctgccccg cttcggcggt ggtactgccg ctcaccgtca cgcccgcatc ggcttcgctg
    21901 gcgttgacga tattgtcgct ggcgacggta ttgataatga tggttggcgc gctgctgtcg
    21961 acgctgtagt tgtgcaccgc cgaggcgcta ttgccgttga tattgctgac gctggccagt
    22021 gcggaggcgc tgccctcggg taatgacgcc agatcggcgg cgggtacggt ggcagtccag
    22081 ctaccatcgc tcgctacgct ggcagtgtaa tttttcccgc caaaggtgac ggtgacggtt
    22141 tgaccgggtt ctacgttagt ggtggtgccg gaaagggtta aatcagcgcc tttttctgcg
    22201 gcgttaatca catcgtccgt ggcgatggtg ttgatggtga tcgctgccgg agtgagatcc
    22261 accgtcaccg gatgcgtaat gctcaccgag tttccggcgc tactttcccc tgaaacggca
    22321 atattaaccg tccccgcagg ccaggcgcta acctgcgccg ccgtgacgcc cacgctccag
    22381 ctaccatcgg cctgcaccgc agtggtgtat tccacattat taatcgtaac ggtaagcggc
    22441 gtgctttcag ccagccccga gctgctgccg gtgaccacca gcgcctgccc gtgctcgatg
    22501 atattgacca catcatcgcc cgccaccgta tcgacccgca ggccgggcag attggcgtcg
    22561 atggtgatat cccgcgtgcc gctgccggtg ttgccgtttt gattggtgac tgaggcatta
    22621 accgttaaat cgccatttcc cagcgcctga atatctgccg ctggaacgtc cacgctccag
    22681 gtgagattgc tgcccaccgt cgccgtataa gtattaccac ccaacgtaat agttaccgtg
    22741 tcgccatctt ccgcgccagt cacctgcccg ctgatggttt gcgccacgcc cgcttcggcg
    22801 gcgttaataa tatcgtcggt tgccaccgga ttaatggtca caccgggcag cgcgctgtcg
    22861 accagcacgt tatggctggc agtggcgctg ttgccgatat cgctggtcac cgccgccgtt
    22921 accgtatagt tggcctggcc taatgctgta accgccgatg ccggaacggt gacgctccag
    22981 ttgccggagg cgtcggtcgt ggtggtgtaa ttttgcccgt tcagcgtcac ggtgatggcg
    23041 gtgttaacag gctgatcgct ggtgccggta atctggagat cggccccttt ttcgctggcg
    23101 ttaatcacat catcgcccgc aatgctgtta atgccgataa ggggcgcggc ggtattgacc
    23161 gtgacggtca atgactggct gcccgtgttt ccggctttgt cggttaccga gacgctgaca
    23221 ggataactgc cgtccgccag cccactgacc accgaggccg gaacgcccag actccagttg
    23281 ccggaaccat ccaccaccgt ggtgtaatcc acattattca gcgtcacggt gacgatatcg
    23341 cccgcctgag cgccagtaac cgtgccgctg atgatttgcg cctgagcatg ttccggcgtg
    23401 ttgatgacgt tatcggtcgc caccggatta atggttacca ccggcggcgt ggcatccacc
    23461 agcgccacgc gtgaaacgct gctcggatta ccggcgacat cgttcacgct ggcggtgacc
    23521 gtagcgttgc cgtcagccag tgcgcccacg ttggcggcgg gcacagtcac gctccaggtg
    23581 ccgtctggct gcacgacgcc ctgatacgtc tgcccattta acgtaacggt caccgtctgc
    23641 cccggctgag cgtcggtggt gccggtgatg ggcagatcct gcccgctttc ggcggcgtta
    23701 atgatgttat cagcggtcag tggatcaatg cttaaggccg gggcctggct atcgacggta
    23761 atggtgcggg aagtgttgcc ggtattgccg ctgagatccg tcacagaagc ggtaatcgcc
    23821 accgcgccgt caggcagatt tcccatcgcg gcggcaggta ccgtcagact ccaggagcca
    23881 tcggcctgca cggtagtggt aaaactctgt ccagccaggg ttactgtgac ggtttgtcct
    23941 gtctcagcct gttgagtgct accggtaagg gtcagtggcg cacctttttc tgcgccactg
    24001 ataatgtcat cgccggagac ggtgttaatg gtgatcaacg gagaaacacc gaccagcgtg
    24061 attgctcgag acgccgcgcc agtatttcct gcagcatctg tcacggtgac gaaaatcgta
    24121 ttggctccgc gatccagcgt gcgggttacc gctgggtcca gcgccacatt ccagctgcca
    24181 tctgccagca cgatgcccgt caggacatga gtgcccaatt tcaccgttac cgtatcgcct
    24241 ggagaagcac cgctgacttg tccggagatg atctgcgcga cggcctgctc ggcattatta
    24301 agaatatcgt cgcccgccac ggtattaaca ctgaccacgg gtgctgaggt gtctaccgtg
    24361 aaattcgcgc tgtggctggt ggtgttccct gcccgatcgc tgacactggc attgacagcg
    24421 taattaccct ccgccagtgc ctggacttcc gtggcaggca gcgtcacttg ccaggttccg
    24481 tcgctaccga cggttgctgt atggcttttc ccgttgacgg tcagtgttac ggtttgcccc
    24541 gcttctgcat tcgacgtgcc gctcagtacc agcgcgacgt tatgttccgc agcactgata
    24601 atgttgtcct gagcgatggt gttgatggca agcgttggcg gttgggtatc gaccgttagt
    24661 agctgcgcgc cggtgaccgt attgcctgtg gcgtctttcc cactgaccag caccgcctga
    24721 ttcccttcgc ctaacgcctg tgcatcggcg gtcggcacct gcacgctcca ggcaccgtta
    24781 gcgcctacgc tggtcgtgta ggttttgccg tttagcgtaa cggtgagttc tgttccctgc
    24841 gccagatggc tactggtgcc agaaagcgta aacccggcgg cctgttcgct ggcgttaata
    24901 atgttatcgc ctgcggtggt ggcaacctgc acgctggcct gactattgtc gatggttaac
    24961 gttatcgaac cggcgtcagt gatggtactg ccgacaattt gcaccactga ccaggtatgt
    25021 tcacctgcac tttgcgtggg taacgagaca ctccagtgac cgttgttgtc tacctgagta
    25081 ctggcaatag tattgccgtt actgtcttta atctgaatag tcgcccccgc ctgcccgctg
    25141 ccgctaaagg ttggcgtggt gtcatcggtg atgtcattgg tggctagaat gccgcgctga
    25201 tcgccctgat tatcggtaac taaaaatgat ggtgtggcgt tgccaggctc agcaggtggc
    25261 gtagggttat taatcacttc tgttttcgag tcgccatcgc caccgcttgc cagcagtgca
    25321 ccaagcgcgc caccacctac cgccgccccc gccagccaac cccacgggaa ggcgctggtc
    25381 tgagcaacgg tatcaagaaa tggcgcaatg ctttcaatcg cagtggtctg ggcagtcagt
    25441 tctacggggg ctaatccacc cgcagcagta tcggcaaagg tgatatgggt tagctgttga
    25501 ccatcggcaa acaccagttc cgattgttca gctgtattcg ccgcttgcag gaaataaccg
    25561 ttgcagcgga tcaccgtgcc gtcctgcata tagatcagca ggtcatttcc ttcacggacg
    25621 taacgcgcaa cggcagaggc ctgagcatga acctgcacca cggaaggctc ataaataaca
    25681 accgtgacgt cgccaggatt aatcagcgtt tgtttgacgg atgttttacg cgaaataaca
    25741 tcaataatca ggctcataat ggcattcctg ataaatggcg cacatcctta gcgagtccat
    25801 ttatctaaat aaggcaaagg taatcactta acaacggccc actattaata atggcaggat
    25861 tatttcaagt cgggtaagtc gttgtataag ccctgtttaa ttcccgctaa tggaataagg
    25921 ttattcaccg cagcggcata atttacggcg gcgacccagc catcataatt ggcatttatt
    25981 tcagccgatt gcgcctgaaa gacatcttgt tcgacggtta atagatcatt cagactgcgt
    26041 ttacccaatt tatattcatt ttggtacaca tctcgcgttt tgtgcgcact ttcggattgc
    26101 gctaaaccgg cttcttcacg accccgtgcg ccggtccagt tcgcatatgc cacagacgct
    26161 cgttgcagca catccagttt tgcctgttcg acctgcgagg cagagatttt ttgttgccct
    26221 tcggcctgtt gcacctgggc agaaaccgcg ccgccctgat acagcggcgc gttaacgttc
    26281 agttgtagct gatcatccca atacgagcgg tcgctggtct ggtagcgcgt tttacccccc
    26341 tgaatactga gcgttggcca gtattgcgct ttcgtttttt ccacgccgta ctgtgctgac
    26401 tggcgtaagt tttctgccgc cagcaccagc gggatggact ggtaatcaat attcttcagc
    26461 gataccggct gctcggctaa ttcagcaggt ggcgcggcta tcgcctccgg ctgtacgcca
    26521 gtgagcaccg ccagttgcgc tttggcgctc gccatctgcg cctgatattg ctccagcgtt
    26581 gagcgcattc cggcaatacg cgtctgggcc tgtaattcat ccgacgatga gttcaggcct
    26641 gcgttagcgc gcaatgccgc catgttgtag acgttttcca gcgagtgaat attgcgttga
    26701 gccgcatcgc acaaagcctg ataacgactg acttccatgt aggcagtcgc cgttttctct
    26761 gcaacatcgg ttaaggttgc catcaattta aagcggtagc tgtcacgggc ggcagtttgc
    26821 agattgatat cgttgttagt tttaccaaag tcatacacca gttgtgtcag ggttatgcca
    26881 tacgaaacgt tgttatcaag cctgccgctg gagtccgttt gtcgggacgg ccccgcgttg
    26941 ccggttaatc caacgtgcgg ataccaggca cttttggctt catcaatttg cgcttcgcca
    27001 atacctatct gcgccgcctg ttgagtgaca ccgggatttc gcgcaaaggc gcgtaatata
    27061 ctttcctgta aagataattg ggcaactggc gttaccgctg gggcattcgc ccattgtggc
    27121 agcggtgcag caattgcagc ttctcccatc accataaaac ttataaaaaa aataagtcca
    27181 ttatttttac ggtttttaaa ctggaatcgc gccatagata agtcacttcc ttccaggtca
    27241 ttattttaac tggtttttat aaccagtctc gttttattaa gaagggtaat aacttgtgca
    27301 tctcttttgt cgatcattga tatgggcaaa gaaatgcagc taaaaagctt atacgttatt
    27361 tatttagaat
//
LOCUS       DAZFDC010000058        27081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFDC010000058 DAZFDC010000000
VERSION     DAZFDC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.058"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(172..1836)
                     /gene="glnS"
                     /locus_tag="ACHJ48_002520"
     CDS             complement(172..1836)
                     /gene="glnS"
                     /locus_tag="ACHJ48_002520"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO4708063.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRSD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2039..3985)
                     /gene="nagE"
                     /locus_tag="ACHJ48_002521"
     CDS             complement(2039..3985)
                     /gene="nagE"
                     /locus_tag="ACHJ48_002521"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO4708064.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4318..5118
                     /gene="nagB"
                     /locus_tag="ACHJ48_002522"
     CDS             4318..5118
                     /gene="nagB"
                     /locus_tag="ACHJ48_002522"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO4708065.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5178..6326
                     /gene="nagA"
                     /locus_tag="ACHJ48_002523"
     CDS             5178..6326
                     /gene="nagA"
                     /locus_tag="ACHJ48_002523"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4708066.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6335..7555
                     /gene="nagC"
                     /locus_tag="ACHJ48_002524"
                     /gene_synonym="nagR"
     CDS             6335..7555
                     /gene="nagC"
                     /locus_tag="ACHJ48_002524"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO4708067.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7603..8355
                     /gene="nagD"
                     /locus_tag="ACHJ48_002525"
     CDS             7603..8355
                     /gene="nagD"
                     /locus_tag="ACHJ48_002525"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO4708068.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8612..10276
                     /gene="asnB"
                     /locus_tag="ACHJ48_002526"
     CDS             8612..10276
                     /gene="asnB"
                     /locus_tag="ACHJ48_002526"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO4708069.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10656..10732
                     /locus_tag="ACHJ48_002527"
     tRNA            10656..10732
                     /locus_tag="ACHJ48_002527"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10690..10692,aa:Met,seq:cat)
     gene            10743..10827
                     /locus_tag="ACHJ48_002528"
     tRNA            10743..10827
                     /locus_tag="ACHJ48_002528"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10777..10779,aa:Leu,seq:tag)
     gene            10851..10925
                     /locus_tag="ACHJ48_002529"
     tRNA            10851..10925
                     /locus_tag="ACHJ48_002529"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10883..10885,aa:Gln,seq:ttg)
     gene            10960..11034
                     /locus_tag="ACHJ48_002530"
     tRNA            10960..11034
                     /locus_tag="ACHJ48_002530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10992..10994,aa:Gln,seq:ttg)
     gene            11050..11126
                     /locus_tag="ACHJ48_002531"
     tRNA            11050..11126
                     /locus_tag="ACHJ48_002531"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11084..11086,aa:Met,seq:cat)
     gene            11175..11249
                     /locus_tag="ACHJ48_002532"
     tRNA            11175..11249
                     /locus_tag="ACHJ48_002532"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11207..11209,aa:Gln,seq:ctg)
     gene            11287..11361
                     /locus_tag="ACHJ48_002533"
     tRNA            11287..11361
                     /locus_tag="ACHJ48_002533"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11319..11321,aa:Gln,seq:ctg)
     gene            complement(11515..12690)
                     /gene="ubiF"
                     /locus_tag="ACHJ48_002534"
     CDS             complement(11515..12690)
                     /gene="ubiF"
                     /locus_tag="ACHJ48_002534"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO4708070.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQVEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12836..14260
                     /gene="miaB"
                     /locus_tag="ACHJ48_002535"
     CDS             12836..14260
                     /gene="miaB"
                     /locus_tag="ACHJ48_002535"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO4708071.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14413..15453
                     /locus_tag="ACHJ48_002536"
     CDS             14413..15453
                     /locus_tag="ACHJ48_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO4708072.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15450..15917
                     /gene="ybeY"
                     /locus_tag="ACHJ48_002537"
     CDS             15450..15917
                     /gene="ybeY"
                     /locus_tag="ACHJ48_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO4708073.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQMVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16007..16885
                     /gene="corC"
                     /locus_tag="ACHJ48_002538"
     CDS             16007..16885
                     /gene="corC"
                     /locus_tag="ACHJ48_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO4708074.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16910..18448
                     /gene="lnt"
                     /locus_tag="ACHJ48_002539"
     CDS             16910..18448
                     /gene="lnt"
                     /locus_tag="ACHJ48_002539"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO4708075.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18845..19753
                     /gene="gltI"
                     /locus_tag="ACHJ48_002540"
     CDS             18845..19753
                     /gene="gltI"
                     /locus_tag="ACHJ48_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO4708076.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            19923..20663
                     /gene="gltJ"
                     /locus_tag="ACHJ48_002541"
     CDS             19923..20663
                     /gene="gltJ"
                     /locus_tag="ACHJ48_002541"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO4708077.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQASKLLDYSAHAWESFTAITLA
                     YVFINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20663..21337
                     /gene="gltK"
                     /locus_tag="ACHJ48_002542"
     CDS             20663..21337
                     /gene="gltK"
                     /locus_tag="ACHJ48_002542"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO4708078.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21337..22062
                     /gene="gltL"
                     /locus_tag="ACHJ48_002543"
     CDS             21337..22062
                     /gene="gltL"
                     /locus_tag="ACHJ48_002543"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO4708079.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22180..23115
                     /gene="rihA"
                     /locus_tag="ACHJ48_002544"
     CDS             22180..23115
                     /gene="rihA"
                     /locus_tag="ACHJ48_002544"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO4708080.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            23199..24869
                     /gene="hscC"
                     /locus_tag="ACHJ48_002545"
     CDS             23199..24869
                     /gene="hscC"
                     /locus_tag="ACHJ48_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO4708081.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPIHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKRTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRQASVYLAIEIP"
     gene            complement(24929..26380)
                     /gene="djlC"
                     /locus_tag="ACHJ48_002546"
                     /pseudo
     CDS             complement(24929..26380)
                     /gene="djlC"
                     /locus_tag="ACHJ48_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(26377..>27081)
                     /locus_tag="ACHJ48_002547"
     CDS             complement(26377..>27081)
                     /locus_tag="ACHJ48_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4708082.1"
                     /translation="NKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSW
                     GINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVA
                     QTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYERAHHYYHSWMHY
                     FSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPL
                     TVQPECPVTLKDVSDL"
BASE COUNT         6642 a         7036 c         6884 g         6519 t
ORIGIN      
        1 tgcggcgtga acgccttatc cggcctacaa aagcacgcaa actcaatata ttgcagagat
       61 catgtaggcc tgataagcgt agcgcatcag gcaatttagc gtttgaaact gtttgtctgt
      121 tccttgccgg atgcgacgta aacgccccat ccggcatagc gaaacttaaa attactcgcc
      181 tactttcgcc caggtatcac gcagcccaac ggtgcggtta aataccggtt tttccgccgt
      241 agaatggcgg ctatcgaggc agaagtaacc ttcacgctca aactggaatg ctttacccgc
      301 aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg attccgggtt
      361 aatcaccgac aggaaatcat ccgcagcacc tgggttcggc acgctgaaca gacggtcata
      421 caaacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac
      481 tttacgacca tctgccggat ctttgcttaa ggtatcggcg tcataagtac agaagatggt
      541 ggtgatatta ccttcggcat ctttctcgac gcgttctgcc ttaatcacat aagcattacg
      601 cagacgcact tctttaccca gcaccagacg tttgtactgc ttgttagctt cttcgcggaa
      661 atcggagcga tcaatccaaa tctcaccgct aaacggcacc tgacggctcc ccatttccgg
      721 tttgttcgga tggttcggca tggtaaccat ttcgccttcg ccctgatagt tttcgataac
      781 cagtttcacc ggatcgataa ccgccattgc gcgcggcgca ttttcgttga gatcttcacg
      841 gatgcaggat tccagcgacg ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg
      901 tttgcagaac tcacgaatag aagccgcagt gtaaccacga cgacgcagac cggaaatggt
      961 cggcatacgc gggtcatccc agccttcaac gtgcttgtcg gtcaccagca agttcagctt
     1021 acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact ggcgcgggtg
     1081 aacaggaatg gtgatgttgt ccagtaccca gtcgtacaga cgacggttat cctggaactc
     1141 aagcgtacac agagagtgcg taataccttc cagggcatcg ctgatgcagt gggtgaagtc
     1201 gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa atttaatgcg
     1261 gtacagcacc ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag
     1321 gcaggcttta ccttcttcaa aaccgccggt acgcattttc tcgaacagcg ccaggttctc
     1381 ttcaacgcta cggtcgcggt acgggctgtt tttaccaggc tgcgtcaggg tgccgcggta
     1441 ttcgcggatc tgttccggcg tcagttcatc aacgtacgcc aggcctttat tgatcagttc
     1501 gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt taccagacca
     1561 gtgaaaacct aaccactcaa cgtcgttttt gatcgaatcg acatactcga tatcttcttt
     1621 taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct gggcgatccc
     1681 gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg gctccggcgg
     1741 gaaacgggtg tgtactgtgg tgtgcttacc actggccaga tcttcatcga tgatctgacg
     1801 gataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa agcgtaaaca
     1861 acgtataacg gcgtatgatc ttataagcgg gacaggctga caactgttag ttagtgaaaa
     1921 tcgaaaatct cactgagatt atggggatga ttgatgaaaa aaaaagcggc agagaagatc
     1981 tccaccgctt gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt
     2041 actttttgat ttcatacagc ggtgtttgac ccgccacaac atggccctga gctttaatga
     2101 tcaagccact gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc gaacgggcgt
     2161 tagcgttcag gtaatccaga tccatttcca gaatcggttg ccctgcgctt acctgcgcgc
     2221 cctcttccac cagacgttta aagcctttac cttccagcgc tacggtatcg atacccatgt
     2281 gaacgacaat ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg gtgttgaaga
     2341 ttttcacgat tgttcctgcg gctggtgata cgacgatttt atctgtcggt ttcaccgcca
     2401 caccgtcacc caccgctttg ctggcgaatg cttcgtcagg aacctgatcc agtgccacga
     2461 catcaccggt aatcggcgat accagctccg cgatagatac cgcgtttggt acagcctgcg
     2521 gttttgctac aggagcggca gttgccggag ttgcttcagc agaggcagct gctaccgggc
     2581 cacgggcaac gactttcttc atcgcatcgc cgatggattc tgctttcgcg ccaacaatca
     2641 cctgaatagt ctgtttgttc agttttacta ccccagaagc ccccagacgt ttacacatcg
     2701 tatcgttaac gcgggcagag tcagccacgg taaggcgcag acgggtgata caggcgtcaa
     2761 ttgctttcag gttgtcagtg ccgccaaccg cagcaatata gttggtcgcc agttgattga
     2821 ggccttcttc agtgttgctg ttggcttctt cagtaacgat ctcgtcttct ttatcttcac
     2881 gacccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg aagtagatag
     2941 cgaagaagac aacacccata accagcagca tccagacgtt ctggctggcg gccggcaggt
     3001 tatacatcaa cgcgtagtcg atagcccccg cagagaaaga gaagccggca tggataccca
     3061 gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg
     3121 gagcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg aacgcagtaa
     3181 cagcaacaga aagcagcatc ccgccaacca tcggacgacg ctctttcggt gctgcgaagt
     3241 acatcgccag cgccgcaccc ggcaggccga acatcatgat cgggaagaag ccggacatga
     3301 acatccccgc ggtgccgtca ccagcgtaga aacggttgat gtcgccgtgg aaaaccgtac
     3361 ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagc acctgatgca
     3421 gaccggttgg gatcaacaga cggttgatga aaccaaagat accggaaccc agcgcgcccg
     3481 cagaaacgat ccactcgccg cctgcatgga tagcgtgctg taccggcggc cagacgtaac
     3541 caaaaatggc cgccagcacc agacagaaga agccggtggc gatcggcaca aagcgtttgc
     3601 cgccgaagaa gctcaggaag tccggcagtt taatatcgga ccaacggtta taggctgcgc
     3661 caccaaccag accggtaatg atacccgcca gtacacccat gttaatttct gggttgatgg
     3721 tcaccatcgc tttggttaac acaaagtaac ctaccgcccc cgccagcgcc gccgcacctg
     3781 cgctgtcttt cgaccagctg gatgccacac cgatggcgaa gattaatgcg aggttatcaa
     3841 aaatcgcacc gcccgcctgg gcaataaacg caacgttaag taaatctggc tgaccgaatc
     3901 gcagcaacag tgccgccacc ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc
     3961 gctggaaaaa acctaaaata ttcatcttat tccccctacg agaaccctat ttggctcgtt
     4021 taaagccgta tttttattgt gctgcaaatt gtactgccga tgttctgtaa tcagattgtt
     4081 agatcatctg ccacagagtg tgtgaaaatt taattcgtat cgcaaattaa acgagtgtct
     4141 tttgtgagtt ttgtcaccaa atatcgttat catcactccc ttttactggc taaaccagaa
     4201 aacttatttt atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt
     4261 tacgcttaaa gatgcctaat ccgccaacgg cttacatttt acttattgag gtgaataatg
     4321 agactgatcc ccctgactac cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc
     4381 aatcgtatca atgcgttcaa accgactgct gatcgtccgt ttgttctggg cctgccaact
     4441 ggcggcacgc caatgaccac ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc
     4501 agctttaagc acgttgtcac cttcaacatg gacgaatatg tcggtctgcc gaaagagcat
     4561 ccggaaagct actacagctt tatgcaccgt aatttcttcg atcacgttga tattccagca
     4621 gaaaacatca accttctcaa cggcaacgcc ccggatatcg acgccgagtg ccgccagtat
     4681 gaagaaaaaa tccgttctta cggaaaaatt catctgttta tgggcggtgt aggtaacgac
     4741 ggtcatattg catttaacga accggcgtct tctctggctt ctcgtactcg tatcaaaacc
     4801 ctgactcatg acactcgcgt cgcgaactct cgtttctttg ataacgatgt caatcaggtg
     4861 ccaaaatatg ccctgactgt cggtgttggt acactgctgg atgccgaaga agtgatgatt
     4921 ctggtgctgg gtagccagaa agcactggcg ctgcaggccg cagttgaagg ttgcgtgaac
     4981 catatgtgga ccatcagctg tctgcaactg catccgaaag ccatcatggt gtgcgatgag
     5041 ccttccacca tggagctgaa agttaagact ttaagatatt tcaatgaatt agaagcagaa
     5101 aatatcaaag gtctgtaatt gttatccctg ccctggctcc ttgctcaggg caatattttt
     5161 taaaatcggg ggtcagaatg tatgcattaa cccagggccg gatctttacc ggccacgaat
     5221 ttcttgatga ccacgcggtt gttatcgctg atggcctgat taaaagcgtc tgtccggtag
     5281 cggaactgcc gccagagatc gaacaacgtt cactgaacgg ggccattctc tcccccggtt
     5341 ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg
     5401 tcagcgtgga aacgctggaa atcatgcaga aagccaatga gaaatcaggc tgtactaact
     5461 atctgccgac gcttatcacc accagcgatg agctgatgaa acagggcgtg cgcgttatgc
     5521 gcgagtacct ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa ggcccgtggc
     5581 tgaatctggt gaaaaaaggt acccataatc cgaattttgt gcgtaagcct gatgccgcgc
     5641 tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg gcaccggaaa
     5701 tggttcctgc ggaagtcatc agcaaactgg caaatgccgg gattgtggtt tctgccggtc
     5761 actccaacgc gacgttgaaa gaagcgaaag ccggtttccg cgcggggatt acctttgcca
     5821 cccatctgta caacgcgatg ccgtatatta ccggtcgtga accgggcctg gcgggcgcga
     5881 tcctcgatga agctgacatt tattgcggta ttatcgctga tggcctgcat gttgattacg
     5941 ccaacattcg taatgctaaa cgccttaaag gcgacaaact gtgtctggtt accgacgcca
     6001 ccgcgccagc aggtgccaac attgaacagt tcatttttgc gggtaaaaca atatactacc
     6061 gtaacggact ttgtgtggat gagaacggta cgttaagcgg ttcatcctta accatgattg
     6121 aaggcgtgcg taatctggtc gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg
     6181 cgacgctcta tccggcgcgt gcgattggcg ttgagaaacg tctcggcaca ctcgccgcag
     6241 gtaaagtagc caacctgacc gcattcacac ctgattttaa aatcaccaag accatcgtta
     6301 acggtaacga ggtcgtaact caataagaga aagtatgaca ccaggcggac aagctcagat
     6361 aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc tgattgacca
     6421 gtacgggcca atctcgcgga ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt
     6481 aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg atcagcaggc
     6541 ctccaccggg ggccgccgcg ctatctctat cgtcaccgaa acccgcaatt tccacgcaat
     6601 cggcgtacgg cttggtcgtc acgacgccac catcaccctg tttgatctca gcagcaaagt
     6661 gctggcagaa gaacattacc cgctgccgga acgtacccag cagacgctgg aacatgccct
     6721 gctgaatgcc attgctcagt ttattgatag ctaccagcgc aaactgcgcg agctgatcgc
     6781 catttccgtt atcctgccag gacttgttga cccggacagc ggcaaaattc attacatgcc
     6841 gcatattcag gtagaaaact gggggctggt agaagcactg gaagagcgtt ttaaagtgac
     6901 ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg gagcactact tcggtgcaag
     6961 tcaggattgc gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat
     7021 ctctaacggg cgcattttta ttggccgcaa cggcaacgtc ggtgaaattg gccatattca
     7081 ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt ggctgcctgg aaactatcgc
     7141 tgccaacgcc gctattgaac aacgggtgtt gaatctgtta aagcagggct accagagccg
     7201 cgtgccgctg gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag
     7261 cctggcctcg gaagtgattg agtatgtcgg tcgtcatctg ggcaaaacca tcgccattgc
     7321 tatcaactta tttaatccgc aaaaaattgt tattgccggt gaaatcaccg aagccgataa
     7381 agtgctgctc cctgctattg aaagctgcat taatactcag gcgctgaagg cgtttcgtac
     7441 taatctgccg gtggtacgtt ctgagctaga ccaccgctcg gcaatcggtg cttttgcgct
     7501 ggtaaaacgc gccatgctca acggtatttt gctccagcat ttgctggaaa attaatgtgc
     7561 ttttatagtg gcgcttattg ttgtcaatat tctgggtagt ccatgaccat taaaaatgta
     7621 atttgcgata tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa
     7681 tttttgcacg ggattatgga taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg
     7741 cagaccgggc aagatctggc gaaccgcttt gccactgccg gtgtcgatgt acctgacagc
     7801 gtgttttata cctctgcgat ggcgaccgcc gatttcctgc gtcgccagga aggcaagaaa
     7861 gcgtatgttg tgggcgaagg cgcactgatt catgaactgt acaaagccgg tttcactatt
     7921 accgatgtga accctgattt tgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg
     7981 atgcataaag cggcctattt cgtcgctaac ggtgcacgct ttatcgccac caacccggac
     8041 acccacgggc gcggttttta tccggcctgt ggcgcgttgt gtgcagggat tgagaaaatc
     8101 tccgggcgca aaccgttcta tgttggtaag ccgagtccgt ggattatccg cgcggcatta
     8161 aacaaaatgc aggcacattc ggaagaaacg gtgattgtcg gcgataacct gcgtaccgat
     8221 attctggccg gcttccaggc aggtctggag acgattctgg tgctttctgg tgtttcgtcg
     8281 ctcgacgata tcgacagtat gcctttccgc cccagctgga tttacccgtc agtcgctgaa
     8341 atcgacgtta tctgaatagc aaccacgtct caggacgtgg tttcttattt ttacccatca
     8401 ttaaaatcat cccattcaat aaaacccgcc aaaaattatc gatccatcaa taaatacatc
     8461 accgcaccct cccctttttc acaaatcagc aatcaccatt acgtttttta ttttttcacc
     8521 gccaaatcgc ttgcgcacag tgctgctttg cggcattttt ttaaacaagc aaacacaaca
     8581 agcaacaaat accaggttaa cggagaaggt tatgtgttca atttttggcg tattcgatat
     8641 caaaacagac gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca
     8701 tcgtggcccg gactggtccg gtatttatgc cagcgataaa gccattctcg ctcacgaacg
     8761 tctgtcaatt gttgacgtta acgcgggggc gcaacctctc tacaaccaac aaaaaaccca
     8821 cgtactggcg gtaaacggtg aaatctacaa tcaccaggca ttgcgcgctg aatatggcga
     8881 tcgttatcag ttccagacag ggtctgactg tgaagtgatc ctcgcgctgt atcaggaaaa
     8941 agggccggaa tttctcgacg acttgcaggg catgtttgcc tttgccctgt acgacagcga
     9001 aaaagatgcc tacctgattg gtcgcgacca tctggggatc atcccactgt atatgggcta
     9061 tgacgaacac ggtcagctgt atgtggcctc agaaatgaaa gccctggtgc cagtttgccg
     9121 cacgattaaa gagttcccgg cggggagcta tttgtggagt caggacggcg aaattcgttc
     9181 ttattatcat cgcgactggt tcgactttga tgcagtgaaa gataacgtga ccgacaaaaa
     9241 cgagctgcgt caggctctgg aagattccgt taaaagccat ctgatgtctg atgtgcctta
     9301 cggtgtgctg ctttctggtg gtctggattc ctcaattatt tccgctatca ccaagaaata
     9361 cgcagcccgt cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc agttacactc
     9421 ctttgctgta ggtctgccgg gttcaccgga tctgaaagca gcccaggaag tggcaaacca
     9481 tctgggcact gtgcatcatg aaattcactt cactgtacag gaaggtctgg acgccatccg
     9541 cgacgtgatt taccacatcg aaacctatga tgtgaccact attcgcgctt caacaccgat
     9601 gtatttaatg tcgcgtaaga tcaaagcgat gggcattaaa atggtgctgt ccggtgaagg
     9661 ttctgatgaa gtgttcggcg gttatcttta cttccacaaa gcaccgaatg ccaaagaact
     9721 gcatgaagag acggtgcgta aactgctggc cctgcatatg tatgactgcg cgcgtgccaa
     9781 caaagcgatg tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca agaaattcct
     9841 cgacgtggcg atgcgcatta acccgcagga taaaatgtgc ggtaacggca agatggaaaa
     9901 acacatcctg cgtgaatgtt ttgagtcata cctgcccgca agcgtggcct ggcggcagaa
     9961 agagcagttc tccgatggcg tcggttacag ttggatcgac accctgaaag aagtggcggc
    10021 gcagcaggtt tctgatcaac agctggaaac tgcccgcttc cgcttcccgt acaacacacc
    10081 gacctcaaaa gaagcgtatc tgtaccggga gatctttgaa gaactattcc cgcttccgag
    10141 cgccgctgag tgcgtgccgg gcggtccttc cgtcgcgtgt tcttccgcta aagcgatcga
    10201 atgggatgaa gcgttcaaga aaatggacga tccgtctggt cgcgcggttg gtgttcacca
    10261 gtcggcgtat aagtaagaaa tgcgaaacag ccccggtgaa atccggggct gtttcagtta
    10321 ttgaggtgct tgttgattct gatatgcagt aaggaaatgt tccgggtttg ccacatcact
    10381 gtttgacaac tcatcactgt aggccagtat ttcatagatt aatttcccac cactattgtt
    10441 aaattccgaa tttcatcctg tatgacaaaa gaatggctgt agggtgggaa aaaacacaaa
    10501 gtaaacaata attgacgaat atagcgccac gctgttcgca acctaaccaa acagtcactt
    10561 tcgagcaatt ttccttgaaa aagaggttga cgctgcaagg ctctatacgc ataatgcgcc
    10621 ccgcaacgcc gataaggtat cgcgaaaaaa aagatggcta cgtagctcag ttggttagag
    10681 cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac catctttttt
    10741 ttgcgggagt ggcgaaattg gtagacgcac cagatttagg ttctggcgcc gcaaggtgtg
    10801 cgagttcaag tctcgcctcc cgcaccattc accagaaagc gttgtacgga tggggtatcg
    10861 ccaagcggta aggcaccggt ttttgatacc ggcattccct ggttcgaatc caggtacccc
    10921 agccatcttc tttgagtaag cggttcaccg cccggttatt ggggtatcgc caagcggtaa
    10981 ggcaccggtt tttgataccg gcattccctg gttcgaatcc aggtacccca gccatcgaag
    11041 aaacaatctg gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac
    11101 aggttcgaat cccgtcgtag ccaccaaatt ctgaatgtgt cgaatacgtt cggcaaattc
    11161 aaaaaccaat ttgttggggt atcgccaagc ggtaaggcac cggattctga ttccggcatt
    11221 ccgaggttcg aatcctcgta ccccagccaa tttattcaag acgcttacct tgtaagtgca
    11281 cccagttggg gtatcgccaa gcggtaaggc accggattct gattccggca ttccgaggtt
    11341 cgaatcctcg taccccagcc acattaaaaa agctcgcttc ggcgagcttt ttgcttttct
    11401 gcgttcactc aatgtcgaat gcgatgttga cacgtcttat ccttcaatgt cggatgcgac
    11461 gctgccgcgt cttatccgac ctacggttgg cacgcatccg gcaatgttgt aaggctacaa
    11521 ccctaacgca tatttcagcg cctgacgttt caacacgcca gcacgctccg ccgccatcaa
    11581 accaagatta cgcacaaaac gcagcggcgg cagattatta ctgaatccgg cataaaacag
    11641 atccataccg ctctgcataa tgaagttatc cgccatgcgc cgcatctggt aacgcttgag
    11701 gacaggataa ctggcccacg cttcgccgta gctgcgggca ttcaccagaa catcaatcag
    11761 ggcatcgaca tcacgataac caagattcac cccctgcccc gccagcggat ggatggtgtg
    11821 cgcggcatca cccaccagcg caagccccgg ctgcacatac tgcaacgcat ggcggcgtgt
    11881 cagcggaaac gcaccagcgg caagcggtgt aacgtaaccc agacgcgacg ggaaatgctt
    11941 cgcgatttcc acctgtaact gcgccatatt catattctgc aactggcgaa tgcgcgccgg
    12001 agagtcgtac cacaccagcg atgcccagtt atcaaacagc ggcagaaacg cgcgcggtcc
    12061 atccggggta aattgctgcc aggtgctatc gccgggatcg ttctcgcact ggacgctaat
    12121 caacatgcac gactgcgcat actgccatgc atgaacgcca attcccgcca tctgccgcac
    12181 ctgcgaattt gcgccgtcgg caccaatcac cagcttcgcg cgaatcactt caccgccttt
    12241 cagctccagc tcctgaagat cattatctcg atgtaatgca atcagcgagg ttggcacacg
    12301 taacgttact ttcggatgcg cttccagcgc ctgccacaat gcctgttgca ggacagtgtt
    12361 ttctaccata tagccaagca acggcaactt taattcggct gcgtcaaata ccacatgcgc
    12421 cgtttcccac tcccacgttt ccagtctgcg gtaaggatgg caacgcatag cctgtactgc
    12481 atcccagacc cctaaccctt taagcaatga taccgaagcc gcgctgatcg ccgagatccg
    12541 cacgtccggt tggctatcag cgacaaacgg tgcaggttct gcgtgctcga tcaccgttac
    12601 cgtaaatccg tgctgtgcca gccccagcgc cagtgcgccg ccgaccattc ctccgccgac
    12661 aatggcaatt tccgttggtt gatttgtcat ggtcaatcat cctgttagca atatgcttta
    12721 agtttacagg atttttaccc ccaagggctg ataacgctca tactggtcac aacggcagca
    12781 aagcattaca ctatgcgccc tgcattcctg gctactattt cgcaagagca agtcgatgac
    12841 caaaaaactc catattaaaa cctggggctg tcagatgaac gagtacgatt catcgaagat
    12901 ggccgatctg ctggatgcca cccacggcta tcaactgacc gacgtggcgg aagaagcgga
    12961 tgtgctgctg ctgaacacct gctcaatccg cgagaaggct caggaaaaag tcttccatca
    13021 gttgggtcgc tggaaactgt taaaagagaa gaatccagac ctgattatcg gcgtcggtgg
    13081 ctgcgtggca tcgcaagaag gcgagcacat tcgccagcgc gcccactatg tcgatattat
    13141 ttttgggccg caaacgctgc accgtctgcc ggagatgatc aactccgtgc gcggcgaccg
    13201 cagcccggtt gtagatatca gcttcccgga aatcgagaag tttgaccgtc taccggaacc
    13261 gcgcgccgaa gggccgaccg cgtttgtctc catcatggaa ggctgcaata aatattgcac
    13321 ctactgcgtg gtgccttaca cccgtggtga agaggtaagc cgtccgtctg acgatattct
    13381 gtttgagatt gcccagcttg cggctcaggg cgtgcgtgaa gtcaacctgc tcggtcagaa
    13441 cgtgaacgcc tggcgtggtg aaaactacga cggcaccacc ggatcgtttg ccgatctgtt
    13501 gcgtctggtt gctgcgatcg acgggatcga tcgtattcgc tttaccacca gccatccgat
    13561 cgaattcacc gacgatatca tcgaagtgta tcgcgacacg ccggagctgg tgagcttcct
    13621 gcatctgccg gtacagagcg gttccgatcg cattctgaac ctgatgggac gtacccatac
    13681 ggcgctggag tacaaagcga tcatccgtaa actgcgtgcg gcgcgtccag atattcagat
    13741 cagttctgac ttcatcgtcg gcttccctgg cgaaaccacc gaagacttcg agaaaacgat
    13801 gaagctgatt gccgacgtca atttcgacat gagctacagc tttatcttct ctgcacgtcc
    13861 gggtacacca gccgccgata tggttgatga tgttccggaa gaagagaaga agcagcgtct
    13921 gtatattctg caagaacgta tcaatcagca agcgatggcg tggagccgcc ggatgctcgg
    13981 caccacccag cgtattctgg tagaaggtac atcgcgtaag agcatcatgg agctttccgg
    14041 gcgtacggaa aataaccgcg tggtcaactt cgaaggcacg ccggatatga tcggtaaatt
    14101 cgtcgatgta gaaattaccg acgtctaccc gaactctctg cgcggtaaag tggtgcgtac
    14161 tgaagatgaa atgggtctgc gcgtggcaga aacaccagaa tcggtgattg cccgtactcg
    14221 caaagaaaac gaccttggcg tgggttatta tcagccgtaa ttctcaggcc cgccgttccg
    14281 gtgggccttg ttttttctct ctttatcccc atctttatcg caatgcttgc gcctttttcc
    14341 tgtggcgtga ataatttcct tataggccgg tgaatgttca gcttccggcg gcatacagat
    14401 aagaggaacg gtttgaacat agacactcgc gaaatcaccc tggagccagc agacaacgcg
    14461 cgtctgttga gcctgtgcgg cccgtttgat gacaacatca agcagctcga acgccgtctc
    14521 ggcatcgaga tcaatcgccg cgataaccac tttaaactga ccggccgtcc gatttgcgtc
    14581 accgctgcgg cagacattct gcgtagcctg tatgtcgata ctgccccgat gcgcggtcag
    14641 attcaggata tcgaaccgga acagatccac cttgcgatta aagaagcacg ggtactggag
    14701 caaagcgcgg agagcgtgcc ggagtacggc aaagcggtca atatcaaaac caaacgcggc
    14761 gtaattaagc cgcgcacgcc aaaccaggcg cagtacatcg ccaatattct cgaccatgac
    14821 attaccttcg gcgttggccc ggcgggtacg ggtaaaacct acctggcagt ggctgcggca
    14881 gttgatgccc tggagcgtca ggaaattcgc cgtattctgc tgactcgtcc ggcagtagaa
    14941 gccggtgaga aactgggctt cctgcctggc gatttaagcc agaaagtaga cccgtatctg
    15001 cgcccgctgt acgacgcgct gtttgaaatg ctgggttttg agaaagtcga gaaactgatt
    15061 gagcgcaacg ttattgaagt cgcaccgctg gcctatatgc gtggtcgtac gctgaacgac
    15121 gcatttatca ttctcgatga gagccagaac actaccatcg aacagatgaa gatgttcctg
    15181 acccgtatcg gttttaactc aaaagcggtt atcaccggcg acgtcacaca gatcgacctg
    15241 ccgcgtaata ctaaatcagg cttacgtcat gccatcgaag tgctggccga tgtcgaagag
    15301 atcagcttta acttcttcca cagcgaagac gtggttcgtc acccggtggt ggcgcgtatc
    15361 gttaacgcct atgaagcctg ggaagaagcc gaacaaaaac gtaaagcggc gctggcggca
    15421 gaacgcaagc gcgaagaaca ggaacaaaaa tgagtcaggt gatcctcgat ttacaactgg
    15481 catgtgaaga taactccgga ttgccggaag agagccagtt tcaaacatgg ctgaatgcgg
    15541 tgatcccgca gtttcaggaa gagtcggaag tgacgattcg cgtggtcgat accgccgaaa
    15601 gccacagtct caatctgacc tatcgcggta aggataagcc gaccaacgtg ctctccttcc
    15661 cgtttgaagt gccgcctggc atggagatgt cgctactggg cgatctggtt atctgccgtc
    15721 agatggttga gaaggaagct caggagcaag gcaaaccact ggaggcgcac tgggcgcata
    15781 tggtggtgca cggcagtctg catttgttag gttacgatca catcgaagat gacgaagcag
    15841 aagaaatgga agccctcgaa acagagatta tgcttgctct gggctatgag gatccgtaca
    15901 ttgccgagaa agaataattt gccagccatt tgactggcag acgtccccgc cgttggtgat
    15961 taacgacggc gttttgatta actaacatga gaacccatac gacgccatga gcgacgacaa
    16021 ttcacacagt agtgacacga taagcaacaa gaagggattt ttctccctgt tactcagcca
    16081 acttttccac ggtgaaccga aaaaccgtga cgaactgctg gcgctgatcc gtgattccgg
    16141 gcagaacgac cttatcgacg aagatacgcg cgatatgctc gaaggggtga tggacatcgc
    16201 agaccaacgc gtccgcgaca tcatgatccc ccgctcccag atgattaccc tgaaacgcaa
    16261 ccagacgctg gacgaatgcc ttgatgtcat catcgagtcc gcccactcac gtttcccggt
    16321 gattagcgaa gacaaagatc acattgaagg gattctgatg gcgaaagact tgctgccgtt
    16381 tatgcgcagc gatgctgaag ccttcagcat ggacaaagtg ttacgtcagg cggttgtcgt
    16441 tcctgaaagt aagcgcgtag accggatgct gaaagagttt cgctctcagc gttaccacat
    16501 ggcgatcgtt attgacgaat tcggtggggt ttccggtctg gtaaccattg aagacatcct
    16561 ggaactgatt gttggtgaga ttgaagacga atatgacgaa gaagatgata tcgacttccg
    16621 tcagctgagt cgtcatacct ggaccgtgcg cgcactggct tccattgaag acttcaacga
    16681 agcgttcggc acccacttta gcgatgaaga agtcgacact atcggtggtc tggtgatgca
    16741 ggcatttggg catcttccgg cacgtggcga aaccatcgac atcgacggtt accagttcaa
    16801 agtggcgatg gccgacagtc ggcgtattat tcaggttcat gtcaaaatcc cggatgactc
    16861 accccagccg aagctggatg aataaaaccg aaactggata gataactaca tggcttttgc
    16921 ctcattaatt gaacgccagc gcattcgcct gctgctggcg ttattattcg gtgcctgcgg
    16981 aacgctggcc ttctctcctt acgacgtctg gcctgcggcg attatttcgc tgatggggct
    17041 tcaggcgttg acctttaacc gccgtccact ccagtctgcc gctattggct tttgctgggg
    17101 atttggcctc tttggcagcg gtattaactg ggtctatgtc agcatcgcga cctttggcgg
    17161 aatgcctggc ccggttaaca tcttcctggt ggtactgctg gcggcgtatt tgtcgctgta
    17221 taccggactg tttgctggcg tgctgtcgcg cctgtggccg aaaaccacct ggctgcgcgt
    17281 ggcgattgcc gcccctgctc tctggcaagt gaccgagttt ctgcgcggtt gggtactgac
    17341 aggcttcccg tggttacagt tcggctatag ccagattgat ggcccgttaa aagggctggc
    17401 accgctaatg ggcgtggaag ccattaactt cctgcttatg atggttagcg gcctgctggc
    17461 actggcgtta gtcaaacgca actggcgtcc gctggtggtg gccgtcgtgc tgtttgccct
    17521 tcccttcccg ctgcgttaca tccggtggtt taccccacaa ccggagaaaa ccattcaggt
    17581 ttcgatggtt cagggcgata ttccgcaatc gctgaaatgg gacgaaggcc agcttcttaa
    17641 tacgctgaag atttactaca acgcaacggc accgctgatg ggcaaatcat cgttgattat
    17701 ctggccggag tcggcgataa ccgatctgga aattaatcag caaccgttcc tcaaagcact
    17761 ggacggtgag ttgcgtgata aaggtagctc gctggtgacc gggattgttg acgcgcgtct
    17821 taataagcag aaccgttacg atacctacaa caccatcatc acactgggta aaggtgcacc
    17881 gtacagctac gaatcagccg atcgctataa caaaaaccat ctggtgccgt ttggcgagtt
    17941 tgtcccgctg gagtcgattc tgcgtccgtt agcaccgttc tttgatctgc cgatgtcgtc
    18001 gttcagccgt gggccatata tccagccgcc gctgtcggca aatggtattg agcttactgc
    18061 ggctatttgc tacgagatca ttctcggcga gcaagtgcgc gataacttcc gcccggatac
    18121 cgactatctg ctgactatct ccaacgatgc gtggtttggt aagtctattg gtccatggca
    18181 gcacttccag atggcgcgaa tgcgtgcgct ggagctggcg cgcccactgt tgcgcagcac
    18241 caacaacggc attacggcgg tgattggccc gcagggtgag attcaggcga tgatcccgca
    18301 gttcacccgc gaggtgttaa ccactaacgt gacgccgacc accggactca caccatacgc
    18361 acgtaccggc aactggccgc tgtgggtgct gacggcactg tttggttttg ctgctgtgtt
    18421 gatgagtctg cgtcagcgac gtaaataatc ccttcttatc gtgccggaat acatcccata
    18481 tttcggcacg ctcaccggat gcggcatcag tgcattatcc ggataacaat attctcctat
    18541 ccaactttgg cacatctatt gctttgttat acaaggcaaa gctttaacca gcatcagtac
    18601 aacccggttg caccacagca atccaccggt agcaccaaaa gagtgcattt taagaatttt
    18661 gcttcggaat ggtgcggcgc acctcccaaa tataaacaat aaaacagcaa tatctttaca
    18721 tttagccaaa ctaaatgtta cctaacaggc acaacactac acaataaagt tgcagatgat
    18781 aacaacacaa acactcacaa cgggtatcca tgcgttctta acgcagaaga taaaggagtt
    18841 ggatatgcaa ttacgtaaac ctgccacagc aatcctggcc ctggcgcttt ccgcaggact
    18901 ggcacaggca gatgacgccg ccccggcagc gggcagtacg ctggacaaaa tcgccaaaaa
    18961 cggcgtgatt gtcgtcggtc accgtgaatc ttcagtacct ttctcttatt acgacaatca
    19021 gcaaaaagtg gtgggttatt cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa
    19081 gaaactcaac aaaccggact tgcaggtgaa actgattccg attacctcac aaaaccgtat
    19141 tccgctgctg caaaacggca cttttgattt tgaatgtggt tctaccacca acaacgtcga
    19201 acgccaaaaa caggcggctt tctctgacac tattttcgtg gtcggtacgc gcctgttgac
    19261 caaaaagggc ggcgatatca aagattttgc cgacctgaaa ggcaaagccg tagtcgtcac
    19321 ttccggcact acctctgaag ttttgctcaa caaactgaat gaagagcaaa aaatgaatat
    19381 gcgcatcatc agcgccaaag atcacggtga ctctttccgt accctggaaa gcggtcgtgc
    19441 cgttgccttt atgatggatg atgctctgct ggccggtgaa cgtgcgaaag cgaagaaacc
    19501 agacaactgg gatatcgtcg gcaagccgca gtctcaggaa gcctatggtt gcatgttgcg
    19561 taaagacgat ccgcagttca aaaagctgat ggatgacact atcgctcagg tgcagacctc
    19621 cggtgaagcg gaaaaatggt ttgataaatg gttcaaaaat cccattccgc cgaaaaacct
    19681 gaacatgaat ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa
    19741 agcactgaac taattacaag aaccaggggc ggaaattcta gccctctcga ttgttacgta
    19801 gcacggacag actatacgcc tgatggtcgt tccccatcgg gcctgaaaac cgcaatacgc
    19861 tgggtaacaa tcttcgaggg tagcagttaa cgctgctacc ctcttttttc tggagtagat
    19921 ttatgtctat agactggaac tggggtattt ttttacaaca agccccgttc ggcaacacca
    19981 cctatcttgg ttggatctgg agtggttttc aggtgacgat cgctttgtcg atctgcgcct
    20041 ggattatcgc tttcctcgtc ggttcatttt tcggtatttt acgtaccgtt ccaaaccgtt
    20101 ttctctccgg tctgggtacg ttgtatgtcg aactgttccg caacgtgccg ttgattgtgc
    20161 aattctttac ctggtatctg gtgatcccgg agctgctgcc ggagaaaatc ggcatgtggt
    20221 ttaaggccga gctggatccc aatattcagt ttttcctttc atccatgctc tgcctggggc
    20281 tgtttaccgc cgcccgtgtt tgcgaacagg tacgcgccgc gattcagtcg ctgccgcgtg
    20341 ggcagaagaa tgccgcgctg gctatgggtc tgacgctgcc gcaggcttac cgctacgtac
    20401 tgctgcctaa tgcttatcgc gttatcgtcc cgccgatgac ctcagagatg atgaacctgg
    20461 tgaaaaactc cgccatcgcc tcgaccatcg gtctggtaga tatggcagcg caggcgagta
    20521 aattgctcga ttactcggct cacgcctggg agtcgtttac cgccattacg ctagcgtatg
    20581 tgtttattaa cgcttttatc atgctggtga tgacgctggt tgaacgtaaa gttcgcctgc
    20641 ctggcaacat ggggggcaaa taatgtacga gtttgactgg agttccattg tcccttccct
    20701 gccatatctg ctcgacgggc tggtgatcac gctgaaaatc accgtcacgg cagtcgtgat
    20761 tggtattttg tggggcacca tgctggcggt gatgcgcttg tccagctttg cgcccgtagc
    20821 gtggtttgcc aaagcctacg ttaacgtatt ccgctcgatt cctttagtta tggttttgct
    20881 gtggttttac ctgatcgtgc cgggttttct gcaaaacgtg ctgggattat caccaaaaaa
    20941 tgatatccgt ttaatttcgg cgatggtggc gttttcgatg tttgaagcgg cctactattc
    21001 agagattatc cgtgccggta ttcaaagtat ttctcgtggt cagtcgagcg ccgcgctggc
    21061 gttgggaatg actcactggc agtcgatgaa actgattatt ctgccgcagg cgttccgcgc
    21121 gatggtgccg ctgctgctca ctcagggcat cgtactgttc caggatacct cgctggtgta
    21181 tgtgttatct ctggccgatt tcttccgtac cgcctcaacc attggtgagc gtgatggcac
    21241 gcaggttgag atgatcctgt ttgccggatt tgtttatttc gttattagcc ttagcgcgtc
    21301 gttgttggtc agctacttga aaagaaggac agcataatga ttaccctgaa aaatgtttca
    21361 aaatggtatg gtcactttca ggtgctgacc gactgctcaa ccgaagtgaa aaaaggcgaa
    21421 gtggtggtgg tttgcggccc gtctggttcc ggcaaatcaa cgctgattaa aaccgtcaac
    21481 ggcctcgaac cagtgcagca aggtgaaatc accgtcgatg gtatcgtggt taacgacaag
    21541 aaaaccgatc tggcaaagct gcgttctcgc gtcgggatgg tattccaaca tttcgagctg
    21601 ttccctcatc tgtcgattat cgaaaacctg accctggcgc aggtgaaagt gcttaaacgc
    21661 gataaagcac cggctcgtga aaaagcattg aaattgctgg agcgcgtggg actttccgca
    21721 catgccaata aatttccggc gcagctttcc ggcggccagc agcagcgtgt ggcaattgct
    21781 cgcgcattgt gtatggatcc tattgcgatg ctgttcgacg aacccacatc ggcactagac
    21841 ccggagatga tcaacgaagt actggacgtg atggtggaac tggcgaacga ggggatgacc
    21901 atgatggtgg tgacccacga aatgggcttt gcccgtaaag tggcgaatcg ggtgatcttt
    21961 atggatgagg gtaaaattgt tgaagactcg ccgaaagatg cgttcttcga tgatccgaaa
    22021 tcggaccgcg caaaagactt cctcgcgaaa atcctgcatt aatcactctg gcgcgcatac
    22081 ccaagtgcgc gccatctcac gctttatcgt ctcgcccctc gtatctgtta catcctggcg
    22141 ttacccttgt cgcaaagaag cacacaacaa ggagcaacaa tggcactgcc aattctgtta
    22201 gattgcgacc caggtcatga tgacgctatc gcaatagttc tcgccctcgc ctcaccagag
    22261 cttgatgtca aagcaattac gtcttccgcc ggaaaccaga caccagaaaa aaccttacgc
    22321 aatgttctgc gtatgctgac cttgcttaat cgcaccgata ttccggtggc aggcggcgcg
    22381 gtaaaaccgt taatgcgtga gttgattatc gcggacaatg tgcacggcga aagcggtctc
    22441 gacggcccgg cattaccgga accgacgttc gcaccgcaaa actgtacggc ggtagagctg
    22501 atggcgaaaa cgctgcgtga aagtgcggaa cctgtcacga ttgtgtctac cggaccgcaa
    22561 actaacgttg ccttgctgct caatagccat ccggaactgc atagcaaaat tgcccgtatc
    22621 gtgattatgg gtggcgctat gggactgggt aactggacgc ctgcggctga atttaatatt
    22681 tacgttgacc cggaagcggc agaaattgtc ttccagtcag ggatcccggt ggtgatggcc
    22741 ggtctggatg ttactcacaa agcacaaatc cacgttgaag acactgagcg tttccgcgcg
    22801 attggcaacc ctgtttcaac cattgttgcc gaactgctgg atttcttcct cgaatatcat
    22861 aaagacgaaa aatggggctt tgtcggcgca ccgctacatg acccatgcac catcgcctgg
    22921 ctgttaaaac cggagttgtt taccactgtt gagcgctggg ttggcgtgga aacacagggg
    22981 aaatataccc agggtatgac ggttgttgat tattattatc tgactggcaa taaaccgaat
    23041 gccactgtaa tggtcgatgt tgatcgtcag ggctttgttg atttactggc tgatcgtctg
    23101 aaattttacg cttaacacac gtcattgatt aagatcacca cgcgttacct gggtggtctt
    23161 aatctgttta tctctttttc tttcaagata taaatgcaat ggataatgca gaactcgcca
    23221 ttggtatcga tctcggtact accaatagtt taattgccgt ctggaaagac ggtgccgcgc
    23281 aattaattcc aaataagttc ggtgaatatt taacaccatc cataattagc atggatgaaa
    23341 ataatcatat tttagtcgga aaaccggctg tatcacggcg tacttcgcat ccggataaaa
    23401 cggcagcgtt atttaaacgt gcaatgggca gtaataccaa ctggcggtta ggcagcgaca
    23461 catttaacgc gccagaactg tcctctttgg tattacgctc attaaaagaa gatgccgaag
    23521 aatttctgca acgtccgatt aaagatgtgg tgatctctgt tccggcttat ttcagcgatg
    23581 aacaacgcaa gcatacccgt ttagcagcgg agttagccgg tttaaatgcg gtacgcttaa
    23641 ttaatgaacc cacagcagct gcgatggcgt atggcctgca tacccaacaa aatacccgtt
    23701 cgctggtgtt tgatctcggt ggcggcacgt ttgacgtcac ggtgcttgag tacgccacgc
    23761 cggtgattga agttcacgcc tccgctggcg acaacttcct cggtggcgaa gattttaccc
    23821 atatgctggt cgatgaggtt ttaaaacgcg cggatgtcgc caggaccacg cttaacgaga
    23881 gtgaactggc agccttgtat gcctgtgtgg aagcggcaaa atgtagcaat caatcgccaa
    23941 tacacattcg ctggcagtac caggatgaaa cgcgggaatg cgaattttac gagaacgaac
    24001 tggaagattt gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa caggcgttgc
    24061 gcgatgcgcg tctgaagccg agtcaaatcg acagtctggt gctggttggc ggcgcgtcac
    24121 aaatgccgct ggtgcagcga atcgccgtgc gtctgtttgg caaattaccg tatcaaagtt
    24181 acgatccgag caccattgtc gcgctgggcg cagcaatcca ggcagcctgc cgcttacgca
    24241 gtgaagatat tgaagaagtg atcctcactg atatttgccc ttactcgttg ggcgttgagg
    24301 ttaaccgcca gggcgtttcc ggcattttct cgccgattat tgaacgaaac accactgtgc
    24361 ccgtgtcgcg tgtagaaact tattcaacca tgcacccgga acaggattct attacggtta
    24421 acgtctatca gggagaaaac cacaaagtta aaaacaacat tctggtggaa tccttcgatg
    24481 tgccgttgaa gagaaccggg gcttatcagt cgattgatat tcgctttagt tatgatatca
    24541 acggtttgct tgaagttgac gtgcttctgg aagacggcag cgttaagtcc agagtgatta
    24601 accacagccc ggtaacattg agcgcgcagc agattgaaga gagtcggacg cggttatccg
    24661 cattgaaaat ttatccgcgc gatatgctca tcaaccgcac ctttaaggcc aaactggaag
    24721 agttgtgggc gcgggcgctg ggtgacgagc gagaagagat tggccgggtg atcaccgatt
    24781 ttgatgcggc gctgcagtca aacgatatgg cccgcgtcga tgaggtcagg cggcaggcga
    24841 gcgtttattt agccattgag atcccataat cacggctgcc ggatgcggcg taaatgctgc
    24901 atccggcatc ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt ttgccgagag
    24961 cataacctat caccagcacc gttaagctga ttttgataat gctggtaatg ctctgtggtt
    25021 tttcatcctc atcatttttt tcttcatggt tttcaggtgt tggctcatcg acatcttcat
    25081 cctccggctc gagttttact ttcgtcaaca gcatccgggt gataatggcc tctaatggat
    25141 gagcatctcc gccttccagc cttgtattaa gacgggccag catctcttgt ttcggaaacg
    25201 cttcaagtac tacctgttcc gctacccgat acaaatccag taacggtgaa tcttcttctg
    25261 tctcgctacc gactctcgat ttccaccacg cccatgcttt gcgtttgaag aatagcgact
    25321 caataaacga ttgacctaac ggcgatagtt ttccgctatg taaaatttcc gcccataaca
    25381 gacgcgtccg ggcgctctca ccaggaacat atttcaacgg tttaccttgc gcatctacca
    25441 tgctccgcgc ttcgataacc atcactaata acgcgaaata atccgggcaa tgttgtgcgc
    25501 tccactgcaa caacaaatta tcggcttgtg tagatgggta acttccccag tacgcgcaga
    25561 gatcggtaag caggctttcc ccctcgccgc agtacaaacg ttgtgcacac tggtaatacc
    25621 gggcatcttc gctttctggt tcttccattt gccactgttc ggctacagga atgagttccg
    25681 ccatacctgt ttgcaccgag ctgtaccagc gcaacagttt gcgatgtaac tttgcatcat
    25741 caggaatgaa ccacggacct tccatcgcca aaaacgcagt gacatagtca ggatggtaac
    25801 ggcagcaacg ttccagcgcg aagtaatact caacagtctg gttctgcaac gcaacgggat
    25861 ataccagaag gccaaggaaa tcgaaacaat caccagcctg aatggcctca agaaaagcct
    25921 ccctttctta ctcatctatt ccttcgctgt cattaaacga atgccagttc aaacgctctg
    25981 ccagcaaaga agcacaattt aatgaaagat atcgcgcttc tatagcgatt gcacacagcg
    26041 gccagcgtaa ttgatcgaca tcctccatgt tccaggtatt taattgctga ataaatttct
    26101 gccaggcgga gggctgaaaa cgatcacttt ctgaatccag taatgccctg aatacacgta
    26161 gtatttcatg ctcagtggca tcatcttttt cttcatcatc tgcttcctcg acaggattta
    26221 ccgccagtcg cagggcctct tcataggcct ggcgtaatgc tttaaaccct tgcggatcgg
    26281 tttcgggatg acataacggt aagcgagcaa gataagcctg gcggataatg tctatttgcg
    26341 tcgtgctttc aatttcgagg atttgccaac atgttttcat aggtcgctca catccttaag
    26401 cgttaccggg cattcaggct ggacagttaa tggctgccag ggaagcgtgg catagaaacg
    26461 atcgcttccc gcagccagct cttcgaacat ccgactcccg gcgctatcgt atttgtacag
    26521 ggttagcgcc tcgcgtagcg aagcgttgtc ttcgcattga gaagattgcc agtagagacg
    26581 ccctagtgaa taggcggaaa aatagtgcat ccagctgtga tagtaatggt gagctcgttc
    26641 atagacgcgc gattgcagcc ataaactctc gtcttccgtc agccacttgt tgagcacacc
    26701 gacccgtgac agaaacccca ttcgaacata atcccaagct ttgattcccc cttccccaca
    26761 acacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg agccaccagc
    26821 aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata agccagctaa
    26881 atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat catcacgatt
    26941 atttattccc caggatgatg agagatcgaa ttcttttatc ccggtataca tttttggcga
    27001 gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa gcgcaaaaag
    27061 aagatattgt tcttccttat t
//
LOCUS       DAZFDC010000059        26570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFDC010000059 DAZFDC010000000
VERSION     DAZFDC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.059"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1336)
                     /gene="djlB"
                     /locus_tag="ACHJ48_002548"
     CDS             complement(<1..1336)
                     /gene="djlB"
                     /locus_tag="ACHJ48_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO4708083.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMNEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQIILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGCKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDD"
     gene            complement(1333..2040)
                     /gene="ybeR"
                     /locus_tag="ACHJ48_002549"
     CDS             complement(1333..2040)
                     /gene="ybeR"
                     /locus_tag="ACHJ48_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO4708084.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2205..3182
                     /gene="ybeQ"
                     /locus_tag="ACHJ48_002550"
                     /pseudo
     CDS             2205..3182
                     /gene="ybeQ"
                     /locus_tag="ACHJ48_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(3252..3734)
                     /gene="ybeL"
                     /locus_tag="ACHJ48_002551"
     CDS             complement(3252..3734)
                     /gene="ybeL"
                     /locus_tag="ACHJ48_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO4708085.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFVMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            3970..6552
                     /gene="leuS"
                     /locus_tag="ACHJ48_002552"
     CDS             3970..6552
                     /gene="leuS"
                     /locus_tag="ACHJ48_002552"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO4708086.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVNTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPQWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6567..7148
                     /gene="lptE"
                     /locus_tag="ACHJ48_002553"
     CDS             6567..7148
                     /gene="lptE"
                     /locus_tag="ACHJ48_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO4708087.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGAVSISQDTASVFRNGQTAEYQMVM
                     TVSASVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQEMIIKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7148..8179
                     /gene="holA"
                     /locus_tag="ACHJ48_002554"
     CDS             7148..8179
                     /gene="holA"
                     /locus_tag="ACHJ48_002554"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO4708088.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYSQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8181..8822
                     /gene="nadD"
                     /locus_tag="ACHJ48_002555"
     CDS             8181..8822
                     /gene="nadD"
                     /locus_tag="ACHJ48_002555"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO4708089.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            8846..9457
                     /gene="cobC"
                     /locus_tag="ACHJ48_002556"
     CDS             8846..9457
                     /gene="cobC"
                     /locus_tag="ACHJ48_002556"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO4708090.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9717..10034
                     /gene="rsfS"
                     /locus_tag="ACHJ48_002557"
     CDS             9717..10034
                     /gene="rsfS"
                     /locus_tag="ACHJ48_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO4708091.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10038..10505
                     /gene="rlmH"
                     /locus_tag="ACHJ48_002558"
     CDS             10038..10505
                     /gene="rlmH"
                     /locus_tag="ACHJ48_002558"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO4708092.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10536..12437
                     /gene="mrdA"
                     /locus_tag="ACHJ48_002559"
     CDS             10536..12437
                     /gene="mrdA"
                     /locus_tag="ACHJ48_002559"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO4708093.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12440..13552
                     /gene="mrdB"
                     /locus_tag="ACHJ48_002560"
                     /gene_synonym="rodA"
     CDS             12440..13552
                     /gene="mrdB"
                     /locus_tag="ACHJ48_002560"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO4708094.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13563..14651
                     /gene="rlpA"
                     /locus_tag="ACHJ48_002561"
     CDS             13563..14651
                     /gene="rlpA"
                     /locus_tag="ACHJ48_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO4708095.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFIQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14791..16002
                     /gene="dacA"
                     /locus_tag="ACHJ48_002562"
     CDS             14791..16002
                     /gene="dacA"
                     /locus_tag="ACHJ48_002562"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO4708096.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16112..16375
                     /gene="ybeD"
                     /locus_tag="ACHJ48_002563"
     CDS             16112..16375
                     /gene="ybeD"
                     /locus_tag="ACHJ48_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO4708097.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16476..17117
                     /gene="lipB"
                     /locus_tag="ACHJ48_002564"
     CDS             16476..17117
                     /gene="lipB"
                     /locus_tag="ACHJ48_002564"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO4708098.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17377..18330
                     /gene="ybeF"
                     /locus_tag="ACHJ48_002565"
     CDS             17377..18330
                     /gene="ybeF"
                     /locus_tag="ACHJ48_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO4708099.1"
                     /translation="MDSNNQIESCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            18539..19504
                     /gene="lipA"
                     /locus_tag="ACHJ48_002566"
     CDS             18539..19504
                     /gene="lipA"
                     /locus_tag="ACHJ48_002566"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO4708100.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(19605..19808)
                     /gene="tatE"
                     /locus_tag="ACHJ48_002567"
     CDS             complement(19605..19808)
                     /gene="tatE"
                     /locus_tag="ACHJ48_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO4708101.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(19937..20725)
                     /locus_tag="ACHJ48_002568"
     CDS             complement(19937..20725)
                     /locus_tag="ACHJ48_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO4708102.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            20818..21201
                     /gene="crcB"
                     /locus_tag="ACHJ48_002569"
     CDS             20818..21201
                     /gene="crcB"
                     /locus_tag="ACHJ48_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO4708103.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21255..21464)
                     /gene="cspE"
                     /locus_tag="ACHJ48_002570"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21255..21464)
                     /gene="cspE"
                     /locus_tag="ACHJ48_002570"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4708104.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(21639..22199)
                     /gene="pagP"
                     /locus_tag="ACHJ48_002571"
     CDS             complement(21639..22199)
                     /gene="pagP"
                     /locus_tag="ACHJ48_002571"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO4708105.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIVQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            22788..24173
                     /gene="dcuC"
                     /locus_tag="ACHJ48_002572"
     CDS             22788..24173
                     /gene="dcuC"
                     /locus_tag="ACHJ48_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO4708106.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24214..24894)
                     /gene="dpiA"
                     /locus_tag="ACHJ48_002573"
                     /gene_synonym="citB"
     CDS             complement(24214..24894)
                     /gene="dpiA"
                     /locus_tag="ACHJ48_002573"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO4708107.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(24863..26521)
                     /gene="dpiB"
                     /locus_tag="ACHJ48_002574"
     CDS             complement(24863..26521)
                     /gene="dpiB"
                     /locus_tag="ACHJ48_002574"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO4708108.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISEVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         6705 a         6684 c         6513 g         6668 t
ORIGIN      
        1 catcgtcaac gggcattggc tgcggttgcg gctccggttc aacagtttgc ttctcggtaa
       61 aattcgccgc ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatttt
      121 ctgcatcaag acggcttacg atcggcatta atgattcttc tgtaggttgg ctggcctgca
      181 ctaattgttt ggcaactaga tataatggcc ataaggtatg ttctgtatca cgcgtgagtg
      241 agatccgcgg cttacatcct ttgtgcaaaa ccatctcgac cagtgaacgc cccagagcag
      301 agagtttttc atcgctgagt atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg
      361 ggacataaag tagcggtttt ccctggtcgt taaccagttc acgagcttca atcatcatca
      421 ccactaatgg gtaataatcg actcgatgct gacggcacca ttgcaacatt aaagcatccg
      481 cctgagtgga gggatactcg tgccagtagt cacaaagttc tgccagcagg ctttcgcctt
      541 ctccacaata gacgcgttgc gcgtattcat aataacgcgc atcttcatta tcaggttcag
      601 cttcccgcca cgaccgggcg taatccagga gttcggggat tcccaggcga cctgcactat
      661 accagcggag taaatttctg tgcagttttg catcatcggg gataatcact gcgcgatgtt
      721 gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgggcg aacatttggt
      781 aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg cagaatgatc tggaagttaa
      841 aaacatttcc cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc
      901 cgacgttttc ctcctgcaac caccgcaatc tttctgctaa taacaccacg cactcgaagg
      961 ataaatgggc agtgttcatg gctattgtgc acagcgacca gcgtaattca tcaatctcat
     1021 tcatcgagca ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt
     1081 cactatcaga ggcaagtaac gcacgaaagg cgagcagaat ttcatgttct gctacctcat
     1141 attcttctgg ttgccaaaca gatttagcag gcgactgcgc aatccggagc gcttcctcat
     1201 acgcctgacg aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca
     1261 gcgccagata agcgcggcgg ataatatcga catcagtcgt ttcctctatg ttgaggatct
     1321 tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg tggggtagtc
     1381 agccagtggt tgccagggta aggtcgcgta atatgcatcg tcactggcaa ataattgata
     1441 aaacatatca ttgccggaat cgtcgtattc tttacgcgct aagaattcac gtaataaagg
     1501 cagattatca tcttcgggag actgccaata ttgccgacca aaggtataac cggcaaagta
     1561 ttgccgccag ttactataat aacgtagagc ccgtagatga atacgcgact ggatccatag
     1621 actttcttct tcactcaacc agttattaag tacgcccatt cgacttaaaa aacccatccg
     1681 gacataatcc catgctttaa ttcctccctc accacagcat tcggctgtgc tggcgacaaa
     1741 ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga tagtcacgcc attcacatgg
     1801 agaataacga aaccagcggt gataaaaccc tgccaaccgc gcggcatgac cgttatcgat
     1861 cattcggtga attgtgcgca gcaggtcatc acggtcatca attccccagg aggaagaaag
     1921 gtcaaaaaag cgtgtaccta aatacatttt gggtgatgaa tgcgaaggta aacagcattc
     1981 attgcgtatt tccatcggag tggaaagtgc aaacaatatt ttttgcgact ccatgtccat
     2041 atgatttatc cttaaatatt cattgaatga atattagccg cattgcgacg ctcgtttatt
     2101 tatacttttc tgacgcgaca taatacgcat aaaataaggc ttgcgcaaaa aatgaaacac
     2161 ataaaaattc aatatatttt atctacaatc ataaggaaat gataatgatt ttcacgtcaa
     2221 gttgctgcga taatttatca atagatgaga ttatcgaacg tgctgaaaaa ggcgattgcg
     2281 aggctcaata tattgttggg ttttattata atcgcgatag cgcaattgac tctccggacg
     2341 acgaaaaggc cttttactag ctgaagctgg ccgctgagca aggtcattgt gaagcacagt
     2401 attccttagg gcaaaagtat accgaggata aaagccgtca taaagataat gagcaagcca
     2461 tcttctggct gaaaaaagct gccctacaag gccatacttt cgcttccaac gcccttggct
     2521 ggatactgga tcgtggagaa gaccccaact ataaagaagc ggttgcctgg tatcagatag
     2581 ccgcggagag cggaatgtct tatgcgcaaa ataatcttgg gtggatgtac agaaatggca
     2641 acggagccgc acaagactat acgctggcat ttttctggta caaacaagct gcattacaag
     2701 gccatagtga cgcgcaaaac aatctggccg atctttatga agacggaaaa ggcgttgctc
     2761 aaaacgagac actcgccgca ttctggtatt tgaaaagcgc acagcagggt aatcggcatg
     2821 ctcagtttca aattgcatgg gattataacg ctggcgaagg ggtggaccag gactataagc
     2881 aagcgatgta ctggtatctg aaggctgccg ctcaggggag cgtcggcgct tacgtcaaca
     2941 tcggttatat gtataaacac ggacaaggcg ttgagaaaga ttatcaggct gcctttgaat
     3001 ggtttacgaa agccgctgaa tgcaatgacg ccactgcctg gtataacctg gccattatgt
     3061 atcactacgg agaaggaaga cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag
     3121 ttcagtcatc cggaaccaga gatgtcagtc aggaaattcg tgagactgaa gatttactgt
     3181 agagataaaa acaagggccg gggaatttca ttcggcccat attctcttta ttgctgtgcg
     3241 aaatcagact attacggctc aaacgggcgt ctctggaact ggtcatgacc acatttcggg
     3301 catagcgtca gcacttccgg tgtgtagatc gggagatgga agtgacattt ctcgcagacc
     3361 agatttccca gcccgaccac ttctccgctg tgataaaccc catgatgatt gaggtcctgg
     3421 aaaacttcgc gccattcaag ctgcgtttta tcggtgatgt ctgccagctc ctgccacaag
     3481 ctttctttaa tcacccgcat aaagacgctg tcagattctt ctttcaggct ctcttcatag
     3541 ctcatgacga actcttccag gtcacgtctg acagctcgcg tcagctcatc gacctcggtt
     3601 cgcgttaact cccctgtttt tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata
     3661 tcacgttcgc cattgcgcag gcgttcgctc agtgacgcaa ccagttcacg gtaatattga
     3721 gcaaccttgt tcatcgtttc atctcccggg tcgttaactg actataataa tagacgttct
     3781 ttgttccttg ctttgctaat acgcccacaa actctgtaaa ttaatgtatg aataatccgg
     3841 gcatcatttc aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt gggctatgct
     3901 atgcggatct gaaaaaccac ctctaacgct acttttgtag ccgtaatgaa aacaggacca
     3961 ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt acagcttcat
     4021 tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa gtattactgc
     4081 ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg taactacacc
     4141 atcggtgacg tgatcgcccg ttaccagcgc atgctgggca aaaacgttct gcagccgatc
     4201 ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa caccgctccg
     4261 gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat gctgggcttt
     4321 ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg ttgggaacag
     4381 aaattcttca ccgagctgta taaaaaaggc ctggtatata agaagacttc tgcggtcaac
     4441 tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg
     4501 cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa aatcactgct
     4561 tacgctgacg agctgctcaa cgatttggat aaactggatc actggccaga caccgttaaa
     4621 accatgcagc gtaactggat cggtcgttcc gaaggcgtgg aaatcacctt caacgttaac
     4681 gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat gggttgtacc
     4741 tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa taatcctgaa
     4801 ctggcggcct ttattgacga atgccgtaac accaaagttg ctgaagctga aatggcgacg
     4861 atggagaaaa aaggcgtcaa tactggcttt aaagctgttc acccattaac gggcgaagaa
     4921 attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc agtaatggcg
     4981 gttccggggc acgaccaacg cgactacgag tttgcctcta aatacggcct gaacatcaaa
     5041 ccggttatcc tggcggctga cggctctgag ccagatcttt ctcagcaagc cctgactgaa
     5101 aaaggcgtgc tgttcaactc tggcgagttc aacggtcttg accatgaagc ggccttcaac
     5161 gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg
     5221 cgtgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat ggtgacgctg
     5281 gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct gccggaagat
     5341 gtggtgatgg acggcattac cagcccgatt aaagcagatc cacagtgggc gaaaactacc
     5401 gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat ggagtcctcc
     5461 tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga ttccgaagcg
     5521 gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc cattatgcac
     5581 ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt gaactctgac
     5641 gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt ctactatgtt
     5701 ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga acgtgacgag
     5761 aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta taccggcatg
     5821 agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt tgaacgttac
     5881 ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat gactctcgaa
     5941 tggcaggaat ccggcgtgga aggggctaac cgcttcctga aacgtgtctg gaaactggtg
     6001 tacgagcata ctgcgaaagg tgatgttgcg gcactgaacg ttgatgcact gactgaagat
     6061 cagaaagcgc tgcgtcgcga tgtgcataaa actatcgcta aagtgaccga tgatatcggc
     6121 cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa caaactggcg
     6181 aaagcaccga ccgatggcga gcaggatcgc gctctgatgc aggaagcact gctggccgtt
     6241 gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca ggaactgaaa
     6301 ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc gatggtggaa
     6361 gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgctaaaat caccgttccg
     6421 gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa
     6481 tatcttgatg gcgttactgt acgtaaagtg atttacgtac caggtaaact cctcaatctg
     6541 gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg ttatctctgg
     6601 cggtgttaat caccgccggg tgtggctggc atctgcgtga caccacgcag gttccttcca
     6661 ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc cgtgcggtgc
     6721 gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg
     6781 ttccatccct gcgtttgggt gctgtatcaa tctcacagga taccgcgtcg gttttccgta
     6841 atggtcaaac tgcggaatat cagatggtga tgactgttag cgcgtcggta ttgattcctg
     6901 gacgagatat ctacccgatt agcaccaaag tcttccgttc attcttcgat aacccgcaaa
     6961 tggcgttagc gaaagataac gagcaggaaa tgatcattaa agagatgtac gaccgtgctg
     7021 ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt tccgacgaag
     7081 aacagacgtc gactacaacg gatactccgg caacgcctgc acgcgtctcc accacgctgg
     7141 gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg aagggctgcg
     7201 cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc aggacgctgt
     7261 tcgtcaggta gctgcggcac aaggattcga agaacaccac actttttcca ttgatcccaa
     7321 cactgactgg aatgcgatct tttcgttatg ccaggccatg agtctgtttg ccagtcgaca
     7381 aacgctattg ctgttgttac cagaaaacgg accgaacgcg gcgatcaatg aacaacttct
     7441 cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta ataaattaag
     7501 caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg tgcaggtgac
     7561 ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg caaaacagct
     7621 caacttagaa ctggatgacg cggcgaatca ggtgctctgc tactgttatg aaggtaacct
     7681 gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg gcaaactgac
     7741 attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcactcctt ttcattgggt
     7801 tgatgctttg ttgatgggaa aaagtaagcg cgcattgcat attcttcagc aactgcgtct
     7861 ggaaggcagc gaaccggtta ttttgttgcg cacattacaa cgtgaactct tgttactggt
     7921 taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata agcatcgggt
     7981 atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagtc agccgcagtt
     8041 acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag attacagtca
     8101 gtcagtgtgg gcagagttgg aagggttatc tcttctgttg tgccataaac ccctggcgga
     8161 cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc acctttgatc
     8221 cggtgcacta tggtcatcta aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc
     8281 gagtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg
     8341 tacagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt actcttgatg
     8401 aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa gagtggcggc
     8461 aggaacaagg gccggacgtg ccgctggcgt ttattattgg tcaggattca ttgctgacct
     8521 ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc gtctgtcggc
     8581 gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg gaagatcatt
     8641 tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat ctggctgaaa
     8701 cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcat
     8761 gtgatgattt attgccggaa ccggtactga cttacattaa ccaacaaggc ttgtatcgct
     8821 gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg gcgaaacaca
     8881 agcgaatgtc gatggtctgt acagcggtca tgcgcccaca cccctgaccg cacgcggtat
     8941 tgagcaagcg caaaatctgc atacactgct agatgatgtt tcctttgacc tggttttatg
     9001 cagtgaactg gaacgggcac agcataccgc gcgactggtt ctcagtgacc gccagctccc
     9061 cgttcatatc atccctgaac tcaacgaaat gttttttggc gactgggaga tgcgacatca
     9121 tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg actggcagca
     9181 tgctatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg aacgctttat
     9241 cgcaaggctt agtgaatatc agcactatca gaatatttta atcgtcagcc atcagggcgt
     9301 acttagtctg ttaattgctc gtttaattgg catgcctgcc gagtctatgt ggcattttcg
     9361 cgttgaccag gggtgttgga gcgccattga tataaaccaa aaattcgcga cgctacgcgt
     9421 cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa
     9481 gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca gactttttcc
     9541 gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct gcggcgcggg
     9601 gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga aatcgcctct
     9661 ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg gaaaacttgc
     9721 agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa ggtcaggaca
     9781 tcatcgcctt agacgttcag ggcaaatcca gcatcactga ctgcatgatc atctgtacgg
     9841 gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag
     9901 cgggcctgtt accgctcgga gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt
     9961 tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat gaactggaaa
    10021 aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa atgccggact
    10081 gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg cccttcgagc
    10141 tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc atactcgaca
    10201 aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc ctcgatattc
    10261 caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg aagctggatg
    10321 gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg
    10381 cggcggagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg gttcgcgtgc
    10441 tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct tatcaccgtg
    10501 agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac tcttttcgcg
    10561 actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt ttggggattt
    10621 tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt cgctttaccg
    10681 actaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg cccagccgcg
    10741 gcattatcta cgaccgtaac ggtatccctc tggccctcaa ccgcactatc taccagatag
    10801 aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg
    10861 ttgatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt
    10921 tcacctccat tccggtgaaa actaacctga ccgaagtaca agtagctcgc tttgccgtca
    10981 atcagtaccg ttttccgggt gttgaagtta aaggctataa acgtcgttac tatccttacg
    11041 gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa gacgtcgaac
    11101 gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc ggtaagctgg
    11161 gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa gaggttgaag
    11221 ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt cccaccgcaa gccggacacg
    11281 atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg ctggcgggta
    11341 gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg ggtgctggcg ctggtttcca
    11401 cgcctagtta tgacccaaac ttgtttgttg acggtatctc cagcaaagat tattccgcct
    11461 tgttgaacga cccgaataca ccgctggtga accgcgccac acagggggtt tatcctcccg
    11521 cgtctacagt taaaccctat gtggcggttt cggcattgag cgccggggtg atcacgcgca
    11581 atacgacgct gtttgaccct ggctggtggc aactgccagg ttcggaaaaa cgttatcgtg
    11641 actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa gaatctgcgg
    11701 ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc gaatggatgg
    11761 gtaaattcgg ttatggtcat tataccggta tcgacctggc ggaagaacgt tccggcaata
    11821 tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag ggtgacacca
    11881 ttccggttgg tatcggtcag ggttactgga cagcgacccc aatccagatg agtaaggcac
    11941 tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg agcaccgccg
    12001 aagacggcaa acaggtgcca tgggtacaac cgcatgaacc gcccgttggc gatattcatt
    12061 ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc cctaacggta
    12121 cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatct ggtaccgctc
    12181 aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc gagcgtttac
    12241 gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg gctgtcgcca
    12301 tgattctgga gaacggtggc gcgggtccgg cggttggtac gctgatgcgc cagatcctcg
    12361 accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcct gcggttgccg
    12421 cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct gggataaagt
    12481 ccatctcgat cccacaatgc tgctgatttt actggcattg ctggtttaca gcgccctggt
    12541 tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg gccaaatcgc
    12601 gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt atgaaggctg
    12661 ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg ctttcggtgc
    12721 catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc agccgtcgga
    12781 aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg acgtttgccc
    12841 gccatcgttg aagaacactg gcatcgcgct ggtgctgata tttatgccca cgctgctggt
    12901 ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc tgtttgtact
    12961 gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg tagcggcgtt
    13021 cattccgatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg taatgatgct
    13081 cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt ctaaaattgc
    13141 tattggctcc ggcggattac gcggcaaagg ctggctgcac ggcactcagt cacagcttga
    13201 atttctcccc gaacgccata ctgactttat cttcgcggta ctggcggaag agctgggatt
    13261 agtgggcatt ttgattctgc tcgctctcta cattctgctg atcatgcgcg ggctgtggat
    13321 agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa tgctgatatt
    13381 attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg ttgtaggggt
    13441 tccgctccca ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg
    13501 gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg
    13561 caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa
    13621 gcgatgatgg tcagcaacag acagtaagtg taccgcagcc tgcggtatgt aacggcccca
    13681 tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg
    13741 attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga tttatccagg
    13801 cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc tctggcgaag
    13861 ctttcgatcc gacacagctg acggcggccc atccaacgct tccgatcccc agctacgcca
    13921 gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat cgcggtcctt
    13981 acggcaacga ccgcgttatt tcgctttctc gcgcagcagc tgaccgtctt aacacgtcaa
    14041 acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg ctttctggtc
    14101 ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca cctcccgatt
    14161 taagcggtgg cgcgggaaca agttcagtgt ctggcccgca gggtgacatt cttccggtca
    14221 gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagc agcggtttcc
    14281 tcggcgcacc aacgacctta gcgccaggtg tactggaagg cagcgaaccg acgcctgctc
    14341 cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca atggtgacac
    14401 cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaggtcggg gccgtaagcg
    14461 atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc gtccccggtc
    14521 gcgtaactca aaatggcgcg gtctggcgga tccagcttgg cccattcgcc aacaaagccg
    14581 aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca tttattacca
    14641 ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa
    14701 gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg cacttttttt
    14761 aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc tcgtatcatg
    14821 aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc acatgccgat
    14881 gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc
    14941 ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt ccgccgcgat
    15001 cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat gaaagccggt
    15061 aaatttaaag aaactgattt agtcactatt ggcaacgacg catgggccac cggtaacccg
    15121 gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc ggtttctcag
    15181 ctgatccgcg gtataaacct gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt
    15241 gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa cgccctgggc
    15301 ctgaaaaata cccacttcca gacggtacat ggtctggatg ctgatggtca gtacagctcc
    15361 gcgcgcgata tggcgctgat cggccaggcg ttgatccgtg acgtaccgaa tgaatactcg
    15421 atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg taacggcctg
    15481 ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga caaagcaggt
    15541 tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc ggtgatgggc
    15601 ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt
    15661 ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga accggtttgg
    15721 tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct gaccattccg
    15781 cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga attgcatgcg
    15841 ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg caaaacgatc
    15901 gaacaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt cggcaaaatc
    15961 attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattgaa cacttgaaag
    16021 tgtaatttcc gtccccatat actaagcatc agtaaaaaac tcccgccttc tggcgggagt
    16081 tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga
    16141 attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca
    16201 ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag
    16261 cagcaaaggc aactaccact cggtatctat cactatcaac gccactcata tcgagcaggt
    16321 tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc
    16381 ttctccgtta cccggcccct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc
    16441 tcccactttt actcattctc cacggagatg ccgttttgta tcaggataaa attcttgtcc
    16501 gccagctcgg tcttcagcct tacgagccaa tctcccaggc tatgcatgaa ttcaccgata
    16561 cccgcgatga tagtaccctt gatgaaatct ggctggtcga gcactatccg gtattcaccc
    16621 aagggcaggc aggaaaggcg gagcacattt taatgcccgg tgatattccg gtgattcaga
    16681 gcgatcgcgg tgggcaggtg acttatcacg ggccggggca acaggtgatg tatgtattgc
    16741 ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag
    16801 tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgccaggtg
    16861 tctatgttgg ggaaaagaaa atttgctcac tgggtttacg tattcgacgc ggttgttcat
    16921 tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt
    16981 gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta
    17041 ataatattgc accacgttta ctggaaaata ttttagcgct actaaacaat cctgacttcg
    17101 aatatattac cgcttaattt catacctcaa tggcccaatt tacactgggt cattactcat
    17161 aacacctgct aatgctttac atctccttcg cgtttgtata ttttctttat cacttgcaga
    17221 aatgcgatgc tacgccttgc tccgtaactc accccattga ttaacttatt gaaatagttg
    17281 attatgatga gcaaaagcgt tccgttccaa ttagacataa gattattgta atttttttaa
    17341 atattatttt tccatgaata aaattggagt aagtgcgtgg atagtaataa tcaaattgaa
    17401 tcctgtttaa gcagaaaatc gtcagaaggt aaaccacaaa tatttacaac cttacgaaat
    17461 attgatctta atcttctgac tatttttgaa gctgtatatg tacataaagg gatcgttaat
    17521 gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc agtctattca gaaactgcgc
    17581 gttatattcc ctgacccatt gtttattcgc aaaggccagg gtgtcactcc taccgcattt
    17641 gcgatgcatc tacatgagta tatcagtcag ggccttgagt ccattcttgg cgcgctggat
    17701 atcgaaggaa gctatgataa gcaacgaacg ataactattg cgaccacccc ctcggtcgga
    17761 gctctggtcc ttcctgtcat ttatcgggcg attaaaactc actatccgca gcttttactg
    17821 cgcaacccgc ccatcagcga cgcggaaaac caactcagtc agtttcaaac cgatctcatc
    17881 atagataaca tgttttgcac caatcgtacg gtgcaacatc atgttctgtt caccgacaat
    17941 atggtgttaa tttgccgcga gggaaatcca ctactctctt tagaagatga cagagagact
    18001 atcgacaacg ctgcacatgt actcctgtta ccggaaggac aaaatttcag cggtctgcgg
    18061 cagagaattc aagagatgtt tccggaccgg cagatcagct tcaccagcta caacattttg
    18121 acaatcgctg cactggttgc caacagtgac atgttagcga ttattcccag ccgtttttat
    18181 aacctgttta gccgttgctg gccgctggaa aaactgcctt ttccgtcctt aaatgaggag
    18241 caaatagatt tctctatcca ctacaacaaa ttcagcctgc gtgatcccat cctgcacgga
    18301 gtaatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag atcacagcgc
    18361 agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg ctttggctgc
    18421 tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt tgataattac
    18481 aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca cgcacgctat
    18541 gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata agatggccct
    18601 tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc cggaatggat
    18661 gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg caatgcgcaa
    18721 aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa
    18781 ccacggcaca gcaacgttta tgatcctggg cgctatttgt acccgccgtt gtccgttctg
    18841 tgacgttgcc cacggtcgcc cggtagctcc tgatgccaat gagccagtga aactggcgca
    18901 gaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc gtgatgacct
    18961 gcgcgatggc ggtgcccaac actttgcgga ttgtattact gccattcggg aaaaaagccc
    19021 gcaaatcaaa attgaaactc tggtgccgga tttccgcggt cgtatggatc gtgctctgga
    19081 tattctgact gcaacgcccc cagatgtgtt caaccataac ctggaaaacg taccgcgtat
    19141 ttaccggcag gtacggcctg gcgcagatta caactggtcg ctgaagctgc tggaacgctt
    19201 taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggtc tgggtgaaac
    19261 caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga cgatgttaac
    19321 gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt acgttagccc
    19381 ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc atgctgcatg
    19441 cggtccgttt gtccgctctt cttaccacgc cgatttgcag gcgaaaggga tggaagttaa
    19501 gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt gaagcgggtt
    19561 ttttgtatcg aacaagatat tgagggagcg tcctgctcgc cacgtcactc tttatgagag
    19621 agcttttcag cctgaagatc aacgtctgcg ccttttttcg ccgcagcatc gtcatcattc
    19681 atcgccttct tgaacccttt aatggccgct ccaaggtctc cgcccagcgt acgtaacttc
    19741 ttagtcccaa acagcagaac gaccagcgcc gcaactacca gcagtttggt aatactaatc
    19801 tcacccatag ataccttctt gacatataac cggcaacaga acgcgcctta aaaaccaaag
    19861 aatattaacg gtcattcgac gcgcgcacac aatagcaatc tgtaacaagg tgaatcaagc
    19921 gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa gcgacggttg tttaagacgg
    19981 gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac ttccgccata atgagtgcag
    20041 gcatttctga cgctgccgca atggtgacgc caaagggatc tataatccgg ctttgcccga
    20101 tatttttgtt cccgcactcc cccgccgcca ccatataaca ggtggtatcc agcgcacgag
    20161 cggcaagcaa cgttgaccag tggtgctctt tgagcggccc gcgaacccag gcggcaggaa
    20221 gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc cagctctgga aagcgtaagt
    20281 cataacaggt catcagaccg accttcatcc cctccacctc gagtaacgga gcaatttcat
    20341 taccagcatc aacacggcgt gattcctgaa tggcaaatgc atcatagaga tgcaatttgg
    20401 cataacgggc gacgatgttt cctgcctgta gcgccaccag catattccat gcgcgccccg
    20461 gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat gttacgttta ctttctcgcc
    20521 gtaaacgtcc gaggaattcg ccttccagca gctgtgctga tttaaccgat agatctgcat
    20581 catgatcatc gcgcgccagc aatgcttccg gcaggacaaa cagcgatacg tcgttttccg
    20641 ccgcctgcgc catcaacgag gcacaaatct cagcgttctt ttcccacaca gatgtaacag
    20701 caaactgtcc tgcagcaacc agcattgcca cctcctgtca gagtcgtaaa attcgccaac
    20761 gacgattatt cggcgttaca cttatcactc atacaaatca aatagcagga ttttgcagtg
    20821 ttacaacttc ttttagcagt ttttattggc ggtggtacgg gaagcgtggc gagatggctg
    20881 ttaagtatgc gatttaaccc gctgcatcag gcgattccgt tggggacgct ggcagcaaac
    20941 ctgattgggg cattcatcat aggaatggga ttcgcctggt tcagcaggat gacgaacatt
    21001 gatccagtgt ggaaagtatt aatcaccacc ggattttgtg gcggtctaac aaccttctca
    21061 acattttcgg cagaagtggt gtttttgtta caagagggcc gctttggctg ggcattactg
    21121 aacgttttcg tcaaccttct ggggtctttt gccatgaccg cactggcatt ctggctgttt
    21181 tcggcctcaa ccgcacacta aagaccaaaa aaaacccgct gattaagcgg gttttgaatt
    21241 cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt ggcaccgtta
    21301 gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt agtctggatt
    21361 gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa accgaatcct
    21421 ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat tacctttaca
    21481 tgaaaaatag acacaaatgc tgtgtcggtt accagtacac caattgtggt acgctttgtc
    21541 cagtgcaact ttactaaaaa gtgataaaaa tcgcgttatt tttgatgttg cttattttag
    21601 ttattgattt taaagaagtt actaaaactt catttgtctc aaaactgaaa gcgcatccag
    21661 gcaaagtaca cattgccatt gttgtaggta cccggaatgt aggtcatctg aaaagtaact
    21721 gggccataac ccacggaggc caatggcagt agaaccggga gagggatgta attccagtta
    21781 tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat gaaaattttc atccgccagc
    21841 ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt ccacgagtcc
    21901 ttaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg cgacaggcca
    21961 aaaccgccac cccacggtcg ctcgttatag cgatcggttt tttctttgtc gtaagcgaaa
    22021 cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttcaggctg ttgccaggtt
    22081 tgtacaatat tttctctaaa cgttgtcatc cactcatctg cgttagcaaa aactttacca
    22141 acgctgatta actgaataaa aacaaaggaa aagatagcga catatttact cacgttcatt
    22201 tgtgaccata aaacatttat caaaaatcta ctactagcat agcaaagcta caattaacat
    22261 aaccttaaca gccccaacat aaagaataat ctgaatagct gctctattta tttccgcttg
    22321 ttcaagttaa atatttccgc gcatcgattt aaaatgacac atcgttttct gcgatgggaa
    22381 tagtcataaa agaaaaagct agttgtttct gacaacaaaa tgcaaccaag aaaaaatgac
    22441 ttatttttta ataagatacc aaaaatcatc ttaacaggga atatattata tatcgtcaac
    22501 ccacgcccat tgcagattat tttttgagca cacaaccgca gctaacacca ttgcaattaa
    22561 caaatttgca tcaatcctcc atcaatttac acacattatt atgtgataat tgccaaccgc
    22621 taaatatgcg ttttgttatc tacgtataaa aacagcaact tcaatgtctt aatggcagtt
    22681 tttcttgatt ttaatcagca ttcatcgcca atttattggg catatttttt ccttaagctt
    22741 taggaatttt ttattattta ctttggggcc tggagacagg aaaaattatg ctgacattca
    22801 ttgaactcct tattggggtt gtggttattg tgggtgtagc tcgctacatc attaaagggt
    22861 attccgccac tggtgtgtta tttgtcggtg gcctgttatt gctgattatc agtgccatta
    22921 tggggcacaa agtgttaccg tccagccagg cttcaacagg ctacagcgcc acggatatcg
    22981 ttgaatacgt taaaatatta ctaatgagcc gcggcggcga cctcggcatg atgattatga
    23041 tgctgtgtgg atttgccgct tacatgaccc atatcggcgc gaatgatatg gtggtcaagc
    23101 tggcgtcaaa accattgcag tatattaact ccccttacct gctgatgatt gccgcctatt
    23161 ttgtcgcctg tctaatgtct ctggccgtct cttccgcaac cggtctgggt gttttgctga
    23221 tggcaaccct atttccggtg atggtaaacg ttggtatcag tcgtggcgca gctgctgcca
    23281 tttgtgcctc cccggcggcg attattctcg caccgacttc aggggatgtg gtgctggcgg
    23341 cgcaagcttc cgaaatgtcg ctgattgact tcgccttcaa aacgacgctg cctatctcaa
    23401 ttgctgcaat tatcggcatg gcgatcgccc acttcttctg gcaacgttat ctggataaaa
    23461 aagagcacat ctctcatgaa atgttagatg tcagtgaaat caccaccact gcccctgcgt
    23521 tttatgccat tttgccgttc acgccgatca tcggtgtact gatttttgac ggtaaatggg
    23581 gtccgcaatt acacatcatc actattctgg tgatttgtat gctgattgcc tccattctgg
    23641 agttcctccg cagctttaat acccagaaag ttttctctgg tctggaagtg gcttatcgcg
    23701 ggatggcaga tgcgtttgct aacgtggtga tgctgctggt tgccgctggg gtattcgctc
    23761 aggggcttag caccatcggc tttattcaaa gtctgatttc tatcgctacc tcgtttggtt
    23821 cggcgagtat catcctgatg ctggtattgg tgattctgac aatgctggcg gcagtcacga
    23881 ccggttcagg caatgcgccg ttttatgcgt ttgttgagat gatcccgaaa ctggcgcact
    23941 cttccggcat taacccggcg tatttgacta tcccgatgct gcaggcgtca aacctgggcc
    24001 gtaccctttc gcccgtttct ggcgtagtcg ttgcggttgc cgggatggcg aagatctcgc
    24061 cgtttgaagt cgtaaaacgc acctcggtac cggtgcttgt tggtttggtg attgttatcg
    24121 ttgctacaga gctgatggtg ccaggaacgg cagcagcggt cacaggcaag taaatagtaa
    24181 tgccggaggc aagtttcctc cggctttatc atgtcaccca ctgtggtata tgcgttgcgg
    24241 tctgccaact ttgccgtgaa caatttcagc aataatcaga tggcggctgg cgcaatattc
    24301 aagataacgc ctggcagtgg tgcgactgat ggtcagtgcc tgcgccaccg tttctgccgt
    24361 atgttgcaca ccaggctctt taaacagttt tcgcaccgcg tttagcgtca aggggtcaat
    24421 gccggtcggc agttcgtctt tgggttcacc gcgagcatag gcattaaaca tctcatcaat
    24481 ttgcttctgg ctggcgctat caatactttc cagcatatgt ttacgctggc ggaaacgggt
    24541 tagcgtttgc cccagccgtt cataggcaat gggcttaatg agataatcaa atacaccaca
    24601 acgtacggct tcagacaccg tttccatatc gctggctgca gtggtaaaca ccacgtcgcc
    24661 gggataatgc gcctgcacca gttcatgcag taaattaatc cctctaccgt caggaagata
    24721 gttatcgagc aagattagcc ccggcttaaa acgctcgatc atcattcggg cctgcgccag
    24781 gtttcccgcc agtaatatct gactgaatcc gggaatgtga cgaatatatt cagcatgcat
    24841 ctctgccagc ggcgtttcgt cctcaacgat caatagggtt aatggagctg tcatttggtt
    24901 tcactttcgg aatatagatt gaaaataagg taccgcaggg atcattatct tcgagagtga
    24961 taacaccacc gcagcgcgtt acgtagctgg caatcaagta caacccaatg ccatgttcac
    25021 cgggctcgtc agcacgcgta ctgaccccct gctcaaatat tttgtctcgt agagactctg
    25081 gaacgccgca gccctgatcg gcgacttcaa tcaccacatc atcgccttca tcgctgagga
    25141 ataattcaac gatcttgttt ccttcatcgc tacgcaggct ggcttcgaag gcgttatcaa
    25201 gtaaattgcc cacaatggct gcaaactcgg tgctatccag tcctggcggc agttgcgaaa
    25261 gctggctacc ggggacaatg atcattttta gccccagttc ccgggcgcgc tgcactttac
    25321 caaaaagcag ccccgccacc tggcgatcgg caaacgcctc gcgcaggctg tcaataagct
    25381 gttgctgggc ctgagactcc ccctgcacca tcgccagcac gcgatcatac tctttcatct
    25441 gcaacagacc attgagcgtc gacatccaat tgagatgctc gtgtcgcaat gtacgaaggc
    25501 tctcaacgta ttgttttatt tgcgtcagtt gcgcattgag ggtggaaatt tcgtctttac
    25561 tgcgaaagct gataatggcc cccagcaaat catcacctga acgaatagct tcccgattgg
    25621 caataacgct cagaccgtta aagttagcca ccacatcctg acgtttttca tcaatctgtt
    25681 cggtaaagaa atcggcgggc ctgaccactt cagcaatggg tttacccaac cattgccgtc
    25741 cgggagagct cagccccagc atctttcttg cgttacgatt gatggcggta atgtaaccat
    25801 gcggatccac cgcaatcagc ccttcataaa ccgaactaaa cagcgcctct tgctgacgga
    25861 ccacgcgtgc gatttgcttt ggctccatgc ccatcatttg ccgacggata tgcgctgcca
    25921 ggaaccacga cagcaacatc agaatcccta acagcacgac aaacacgcct gccatcggta
    25981 ataaaaactc agcccgccag ctatcgattt tactcaccag gtagccaatc gacaccacgc
    26041 cgatgacttt tccatcgtca tcaaagattg gcgttttggc gcgcatcgcc atccccattg
    26101 accctttccc ggtaatgaag tagctctccc ctttctccag cgcgccctgt ttggtgaact
    26161 gcataggata accaattttc tccggattag gatggtaaag gcggatcgag tgccggtccc
    26221 caatcaccac ataatcaaaa tcggtatctc tttgtaattt gttagcgatg gtcgccagcc
    26281 gtttgtagtc acgcgttttc acctcagaga tgacactgtc attggaggca ataattttcg
    26341 cctgattcat tgccatgtcg cgtacatgaa gcgttaaata gtcctcaaaa ctggccgtaa
    26401 aatattgcgc cagggctgca atgacaaata ttgagaacac cagaatcagc aaaaagatac
    26461 gcagcggaaa tgccagtctt tggaaaaatg caaactgttt attctcgtta agctgcaaca
    26521 ttgttttcct tgcgggttgt cccgagcctc tttgacggat gtaattaaaa
//
LOCUS       DAZFDC010000060        26068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFDC010000060 DAZFDC010000000
VERSION     DAZFDC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.060"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHJ48_002575"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHJ48_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO4708109.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHJ48_002576"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHJ48_002576"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO4708110.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVND"
     gene            complement(938..1405)
                     /locus_tag="ACHJ48_002577"
     CDS             complement(938..1405)
                     /locus_tag="ACHJ48_002577"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO4708111.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHJ48_002578"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHJ48_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO4708112.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHJ48_002579"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHJ48_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO4708113.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHJ48_002580"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHJ48_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO4708114.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHJ48_002581"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHJ48_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO4708115.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHJ48_002582"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHJ48_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO4708116.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHJ48_002583"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHJ48_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO4708117.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHJ48_002584"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHJ48_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO4708118.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHJ48_002585"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHJ48_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO4708119.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHJ48_002586"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHJ48_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO4708120.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHJ48_002587"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHJ48_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO4708121.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHJ48_002588"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHJ48_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO4708122.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHJ48_002589"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHJ48_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO4708123.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHJ48_002590"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHJ48_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO4708124.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHJ48_002591"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHJ48_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO4708125.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHJ48_002592"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHJ48_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO4708126.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHJ48_002593"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHJ48_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO4708127.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHJ48_002594"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHJ48_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO4708128.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHJ48_002595"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHJ48_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO4708129.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHJ48_002596"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHJ48_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO4708130.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHJ48_002597"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHJ48_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO4708131.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHJ48_002598"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHJ48_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO4708132.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHJ48_002599"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHJ48_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO4708133.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHJ48_002600"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHJ48_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO4708134.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHJ48_002601"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHJ48_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO4708135.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHJ48_002602"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHJ48_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO4708136.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHJ48_002603"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHJ48_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO4708137.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHJ48_002604"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHJ48_002604"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO4708138.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHJ48_002605"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHJ48_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO4708139.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHJ48_002606"
     CDS             15545..15913
                     /locus_tag="ACHJ48_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO4708140.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHJ48_002607"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHJ48_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO4708141.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHJ48_002608"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHJ48_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO4708142.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHJ48_002609"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHJ48_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO4708143.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHJ48_002610"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHJ48_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO4708144.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHJ48_002611"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHJ48_002611"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO4708145.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKSMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDTWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHJ48_002612"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHJ48_002612"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO4708146.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHJ48_002613"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHJ48_002613"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO4708147.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ACHJ48_002614"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ACHJ48_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO4708148.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ACHJ48_002615"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ACHJ48_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO4708149.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHJ48_002616"
     CDS             23092..23634
                     /locus_tag="ACHJ48_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO4708150.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHJ48_002617"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHJ48_002617"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO4708151.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHJ48_002618"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHJ48_002618"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO4708152.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKPTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHJ48_002619"
     CDS             25031..25288
                     /locus_tag="ACHJ48_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO4708153.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHJ48_002620"
     CDS             complement(25264..25818)
                     /locus_tag="ACHJ48_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO4708154.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6690 a         6178 c         6762 g         6438 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggcgcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc
      901 tacaaaatta cctgcaatca caaattaaag ttaatgatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttctg cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaagac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggc aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgc gacggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt cgaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cataaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttctgaaggt accggtatca
    10141 tcgccggagg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgtt acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgccgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga
    15721 acttgagcaa cggagggaag caatccagct tctggatatc ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ccgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact ggcaggatta cactcacgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgcgt agagaaaaat
    16501 aagaagggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gccagtggca agaaagtaaa tcactttttt accactggtc ttctgctttc aggggcttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagtg gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaagcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag cgaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggtacag catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc
    18421 aaggttcgca gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttctggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgggaaggg taacggccat caccttgttt cacggttgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca
    19021 accgcaacaa cctgcgcttc tggtgccact tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcat ccagcaatgc aagccaggtg tcgcgggaat gatgcgtacg attaatacgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gttaatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attgacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    19921 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat gccatcacca gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttccacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgcct
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagta tcagcagctt
    21541 gtacggcgat gatatggtcc gtatagctca ctggctggca agacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 gcggaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggtgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagca
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtagcgc ataaagcagc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 ccgccgtcat gcccgactgg ctgccagtct gcttgaacag gggggcgctc tcgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgtagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgaga agtttttgcc ttgccaggtc caatagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    22861 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcattaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgtc cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacgcg cagcgttggc tggtgctgtt aacaccctca
    24481 agcggttaga agatggacgc ttgctgggtg acaataccga tggtgtaggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgcaggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta
    24661 atcggacggt atcccgagcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgccacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc
    24901 gaggctcaaa gcctactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgatagcctg gcgtatcgct ttacaggaga tacgccagag cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacttgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26041 gtcgtaacaa cgaagagaaa aaatcctg
//
LOCUS       DAZFDC010000061        25901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFDC010000061 DAZFDC010000000
VERSION     DAZFDC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.061"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(149..229)
                     /gene="shoB"
                     /locus_tag="ACHJ48_002621"
     CDS             complement(149..229)
                     /gene="shoB"
                     /locus_tag="ACHJ48_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO4708155.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(651..1031)
                     /gene="acpS"
                     /locus_tag="ACHJ48_002622"
     CDS             complement(651..1031)
                     /gene="acpS"
                     /locus_tag="ACHJ48_002622"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO4708156.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(1031..1762)
                     /gene="pdxJ"
                     /locus_tag="ACHJ48_002623"
     CDS             complement(1031..1762)
                     /gene="pdxJ"
                     /locus_tag="ACHJ48_002623"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO4708157.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1774..2502)
                     /gene="recO"
                     /locus_tag="ACHJ48_002624"
     CDS             complement(1774..2502)
                     /gene="recO"
                     /locus_tag="ACHJ48_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO4708158.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2514..3419)
                     /gene="era"
                     /locus_tag="ACHJ48_002625"
     CDS             complement(2514..3419)
                     /gene="era"
                     /locus_tag="ACHJ48_002625"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO4708159.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVATIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3416..4096)
                     /gene="rnc"
                     /locus_tag="ACHJ48_002626"
     CDS             complement(3416..4096)
                     /gene="rnc"
                     /locus_tag="ACHJ48_002626"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO4708160.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4369..5343)
                     /gene="lepB"
                     /locus_tag="ACHJ48_002627"
     CDS             complement(4369..5343)
                     /gene="lepB"
                     /locus_tag="ACHJ48_002627"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO4708161.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5359..7158)
                     /gene="lepA"
                     /locus_tag="ACHJ48_002628"
     CDS             complement(5359..7158)
                     /gene="lepA"
                     /locus_tag="ACHJ48_002628"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO4708162.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7356..7835)
                     /gene="rseC"
                     /locus_tag="ACHJ48_002629"
     CDS             complement(7356..7835)
                     /gene="rseC"
                     /locus_tag="ACHJ48_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO4708163.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7832..8788)
                     /gene="rseB"
                     /locus_tag="ACHJ48_002630"
     CDS             complement(7832..8788)
                     /gene="rseB"
                     /locus_tag="ACHJ48_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO4708164.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8788..9426)
                     /gene="rseA"
                     /locus_tag="ACHJ48_002631"
     CDS             complement(8788..9426)
                     /gene="rseA"
                     /locus_tag="ACHJ48_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO4708165.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHEWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9459..10034)
                     /gene="rpoE"
                     /locus_tag="ACHJ48_002632"
     CDS             complement(9459..10034)
                     /gene="rpoE"
                     /locus_tag="ACHJ48_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO4708166.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(10031..10186)
                     /gene="rseD"
                     /locus_tag="ACHJ48_002633"
     CDS             complement(10031..10186)
                     /gene="rseD"
                     /locus_tag="ACHJ48_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO4708167.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10442..12064
                     /gene="nadB"
                     /locus_tag="ACHJ48_002634"
     CDS             10442..12064
                     /gene="nadB"
                     /locus_tag="ACHJ48_002634"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO4708168.1"
                     /translation="MNTLLEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDISTLPPWDESRVENPDERVIIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(12049..12786)
                     /gene="trmN"
                     /locus_tag="ACHJ48_002635"
     CDS             complement(12049..12786)
                     /gene="trmN"
                     /locus_tag="ACHJ48_002635"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO4708169.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAATQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12918..14252
                     /gene="srmB"
                     /locus_tag="ACHJ48_002636"
     CDS             12918..14252
                     /gene="srmB"
                     /locus_tag="ACHJ48_002636"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO4708170.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14285..15166)
                     /gene="yfiE"
                     /locus_tag="ACHJ48_002637"
     CDS             complement(14285..15166)
                     /gene="yfiE"
                     /locus_tag="ACHJ48_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4708171.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            15269..15856
                     /gene="eamB"
                     /locus_tag="ACHJ48_002638"
     CDS             15269..15856
                     /gene="eamB"
                     /locus_tag="ACHJ48_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO4708172.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFHQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(15912..16295)
                     /gene="grcA"
                     /locus_tag="ACHJ48_002639"
     CDS             complement(15912..16295)
                     /gene="grcA"
                     /locus_tag="ACHJ48_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO4708173.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16600..17289
                     /gene="ung"
                     /locus_tag="ACHJ48_002640"
     CDS             16600..17289
                     /gene="ung"
                     /locus_tag="ACHJ48_002640"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO4708174.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(17337..18374)
                     /gene="yfiF"
                     /locus_tag="ACHJ48_002641"
     CDS             complement(17337..18374)
                     /gene="yfiF"
                     /locus_tag="ACHJ48_002641"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4708175.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18581..19000
                     /gene="trxC"
                     /locus_tag="ACHJ48_002642"
     CDS             18581..19000
                     /gene="trxC"
                     /locus_tag="ACHJ48_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO4708176.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19069..19767
                     /gene="tapT"
                     /locus_tag="ACHJ48_002643"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19069..19767
                     /gene="tapT"
                     /locus_tag="ACHJ48_002643"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO4708177.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19799..22459
                     /gene="pat"
                     /locus_tag="ACHJ48_002644"
     CDS             19799..22459
                     /gene="pat"
                     /locus_tag="ACHJ48_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO4708178.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATEIAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQSYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22573..23928
                     /gene="pssA"
                     /locus_tag="ACHJ48_002645"
     CDS             22573..23928
                     /gene="pssA"
                     /locus_tag="ACHJ48_002645"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO4708179.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23974..24297
                     /gene="yfiM"
                     /locus_tag="ACHJ48_002646"
     CDS             23974..24297
                     /gene="yfiM"
                     /locus_tag="ACHJ48_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO4708180.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKEFWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24294..25592)
                     /gene="kgtP"
                     /locus_tag="ACHJ48_002647"
     CDS             complement(24294..25592)
                     /gene="kgtP"
                     /locus_tag="ACHJ48_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO4708181.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDSALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25709..>25901)
                     /locus_tag="ACHJ48_002648"
     CDS             complement(25709..>25901)
                     /locus_tag="ACHJ48_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708182.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRFPQLAYFHT
                     CPPYQKFILKLLNNHFPAN"
BASE COUNT         6268 a         6837 c         6463 g         6333 t
ORIGIN      
        1 ttaacgccat tatatttatt taattgatga cattagcata ataattcact aagttaattt
       61 atatagtatc tgcccagaca cttatttata gttattaaag gcgcgtccga ttggttcacc
      121 ggacgcacct taagtacgtt tccttgtgtt ataagaacag aaggatcagc tgtaaaacag
      181 caatgatgat tttgatgacc cgtttaatca ggtatcggca atcagtcatt cgtttttcct
      241 taaacaagga atgcagccgt tcagattagc ccttacatct ccccaaaact gaacgtgcga
      301 gttattggaa tcgagtgctg cactccacac cagagctttg acgacaccac tcgtttcaat
      361 gggggaattc agtggcatgg tgtaaagcac agcaaaattg ccaataacga aaccgatttt
      421 aatgtactta tcaaaaagcg gtcacgttaa tcttttcatc aatgtcgatt tcttcgtaaa
      481 ggctcgcgtt ttttatgtca gagattgcaa gttgcttgta caggtcaacg aaattgatcc
      541 ataatcattt tcgttggaat cgtgtgctgc acaaaattaa agttaaaaag taaaaccccc
      601 gttccttacc agttcggggg ttttacttta taaagagaac ggtattattt ttaactttca
      661 ataattaccg tggcacaagc atagtggcgc tcatcggcca gcgtcacatg catatttgca
      721 acgcccagct tttccgccag ttttaatgcc tcgccccaca gtcgcagccg tggcttacca
      781 agctcatcgt taaatacttc aaactgatta aacgccagac cattgcggat cccggtgcca
      841 aacgcttttg ctgcggcttc ttttacggca aagcgtttcg ccagaaaacg caccggctgg
      901 tggtgcgttt tccaaatttc ccattcgtta tcgcttaata cgcggcgtgc caggcgctca
      961 ccggatcggg cgatcactgc ttctatgcga gcgatctcta cgatatccgt gcctaaacct
     1021 aatattgcca ttagccacgc gcttccagca tcaggcgctt catttctgcc accgcatctt
     1081 tcagtccggt catcactgca cgaccaataa tggcatgacc gatattcagt tcgtgcatct
     1141 cagggatggc ggcaatggct tttacgttgt gataggtcag accgtgtccg gcgttaactt
     1201 tcaaaccgag actagcggca aaggtcgcgg ctttggcgat acgcgccagc tcttgcgcct
     1261 gttcggcgtc agttttggca tcagcatagc aaccggtgtg gatctcgata aacggtgcgc
     1321 caacctctgc cgcagctttg atctgctctt catcggcgtc gataaacaga gaaacctgaa
     1381 tcccggcatc cgccagacgt ttgcaggcat cgcgcatttt gtcacgctgc cctgcaacgt
     1441 ccaggccgcc ttcggttgtt acttcctgac gcttttctgg taccaggcag caaaaatgcg
     1501 gcttcgtctc aaccgcgatc gccagcatct cttcggtcac cgccatctcc agattcatgc
     1561 gggtatccag cgtctgacgc aggatgcgca catcgcggtc agtaatgtgg cggcgatctt
     1621 cacgtaaatg cacggtaatg ccgtccgccc ccgcctgctc ggcaataaac gcggcctgca
     1681 ccggatccgg gtaagcggta ccgcgcgcgt tgcgcagcgt agcgatatgg tcaatgttga
     1741 cgcctaacag taattcagcc atgacaatcc tcatcattca taatgtgttt tcaccgttcg
     1801 cttaggcata aattgccgga acagttccct gctctttaaa ggtttaccgc caagatacgg
     1861 cttaagcgcc atgcgggtaa agcgtttcgc ggcgcgcagt gtgtctgcgt caggaaattc
     1921 ccgtgcgttt aacgctttta actgccttcc ggtgaacgtt ttattgtcga taacgacgct
     1981 tgcgataaac cctttttctt cgcgataacg atacgtcatg gtgtcatcta ccggctcgcc
     2041 gctacccgca caatgggtaa aattgacgcc ataacccaga tgcccgagca gtgccagttc
     2101 aaagcggcgc agcgcgggtt ctggcgtacc agtgacccct gcaagagact gaatgcagtg
     2161 caagtaatcg aaaaagagtt cagagaagcg cgtctcgtat tccagtacgc gggagagaag
     2221 ttcgttgatg tacagaccgc tgtaaagcgt gataccgctt aatggcagcg ccagcgagac
     2281 agcttcagca ctgcgcagcg ttttgacttc gccacgcccg ccaaaacgta gcaagagagg
     2341 ggtgaaaggc tgtaatgcac ctttcagggt agagcgttta gagcgtgcgc ctttggcaac
     2401 cagacgcacg cgacccgatt cctccgtgaa gacgtccagc atcaggctgg tttcgctcca
     2461 cgggcgacta tgcaggacaa atgcgcgctg ccagccttcc atcggagtta ctcttaaaga
     2521 tcgtcaacgt aaccgagact gcgcagtgcg cgttcgtcgt cggcccaacc ggatttcact
     2581 tttacccaca gctcaaggtg aacaggcgct tcaaacattt cctgcatgtc tttacgcgct
     2641 tcaatcccga tggttttgat cttggcccct ttgttgccaa tgaccatctt cttctgccct
     2701 tcacgctcaa cgagaatcaa accgttgatg tcataaccac cgcgttcgtt agagacgaaa
     2761 cgttcgatct ccacggtcac ggagtacggc agttcagcgc cgaggaaacg catcagtttt
     2821 tcgcggatga tttcagacgc cataaaacgc tgtgagcgat cggtgatgta atcttccggg
     2881 aagtgatgag tcgcttcagg tagatgctta cgcacgattg ccgcaatagt ggcaacattc
     2941 agcccggttt cggcagagat tggcacgata tcgaggaagt tcatctggct tgccaggaac
     3001 tgcaggtgcg gcagcagatc ggctttctcc tgcacgttgt ccactttgtt caccgcgagg
     3061 attaccggcg ctttgccttc gcgcagtttg ttgagcacca tttcgtcgtc cggcgtccag
     3121 cgggtgcctt caacgacaaa aatcaccagc tcaacatcgc caatagagct gctcgccgct
     3181 ttgttcatca ggcggttaat agcgcgtttt tcttccatat gcaggcccgg tgtatcgacg
     3241 tagatcgcct gatacgcgcc ttcagtatgg atccccacaa tgcggtgacg agttgtctgc
     3301 gccttgcggg aggtgatgga gattttctgc cccagcagtt tgttcaacaa tgtggatttg
     3361 ccaacattcg gacgtccgac gatggcaata aatccgcagt aacttttatc gatgctcatt
     3421 ccagctccag ttttttcaac gcctgttcgg cggcagcctg ctcagcctta cgacggcttg
     3481 aacctgtgcc aaccaccggt tcactcaggc cgctgacctg gcagtggatt gtaaattcct
     3541 gatcgtgcgc ttcgccacgg acctgaacta ccagataagt cggcagcggc agatgacgac
     3601 cctgcaaata ttcttgcaag cgcgttttcg gatctttttg tttatcgcct gggctaattt
     3661 cgtccagacg agtttgatac cagttgagga ttaatttctc gacggtttga atatcactgt
     3721 cgaggaatac gccaccaatt aatgcttcga cggtgtcggc gagaattgac tcacgacgaa
     3781 atccaccgct tttaagttca cctggtccta aacgtaagca ctcgcctaac tcaaattcgc
     3841 gcgccagttc cgccagcgta ttgccacgga ccagcgtggc gcgcatccgg ctcatatcgc
     3901 cttcatccac acgagggaaa cggtgataaa gcgcattggc gataacgtag ctcagaatag
     3961 agtcgcctaa aaattctaaa cgctcgttat gtttgctgct ggcactacga tgagttaatg
     4021 cctgctgcaa cagttcctga tgattaaaag tgtagcccag cttccgttga agccgattaa
     4081 ttacgatggg gttcatgcga taccaataaa taaatgcgtc aacaattcag cacacgaaac
     4141 agacctgata tacatggctc tgctaactgc ttcgctgcag tttcagttta gcggtatatg
     4201 gggaccaacg ctgtttcgtg tgccgtggca acctggaggt gccaacctta aacttcgggg
     4261 gaatattcta tacacaacga cgggggatgt cgttagccac gggagattta tctcataaat
     4321 aattcacgtt gtcgccataa cggcgacaac gtgaacgaag atgagctatt aatggatgcc
     4381 gccaatgcga cttaagcgca cacctgtcgg ccattcgcct tcttgcttat cgaagctcat
     4441 ccagatagcc gttgcccgac cgaccagatt cgcttcaggc acaaagcccc agtaacggct
     4501 gtccgcgctg ttgtcgcggt tgtcgcccat catgaagtat tgtcccggag gaacaatcca
     4561 ggttgccagt tgttgccctg gctgctggta atacatcccc acctgatcct gcgcaatcgg
     4621 cactgtcaga atgcggtgcg tcacatcacc cagtgtctct ttacgctcgg aaagacgaat
     4681 tccattttct ttggtttcgt ttttcggcac ttcaaagaat ccgctggtcg cttccccacc
     4741 attacggcgt gagaaggtct gaacgaaatc gctcggttcc acgtttgagt aggtgaccgg
     4801 cagcgcgttt tcacacgcct ggccggaact gcatcccggt tgaatcgtca gctcttttga
     4861 gaccggatcg taagtgactt tatcgcccgg taaacccacc gcgcgcttga tataatcaag
     4921 ctttggatct tccggatatt taaagaccac gatatcgccg cgtttcggat gaccggtttc
     4981 gatcagcgtt ttctggtaga taggatcttt aatgccataa gcaaacttct ctaccagaat
     5041 aaaatcaccg attaacagag tcggcatcat cgaacctgac ggaatctgga acggttcata
     5101 aataaacgaa cgcacaatca atacgatagc cagtaccgga aaaacagaag caccggtttc
     5161 cagccagcca ggcttcggcg caaccttttt caacgttgct ttatccagtg agtcaccggc
     5221 agccgcctgc gccgctgcct gacgttcccg ccgtttaggt gcgaaaaaga atttatccac
     5281 gcaccataaa atgcccgtca ccagtgtggc aatcaccaga atcagggcaa acatattcgc
     5341 catgccaact cctaagggtt atttgttgtc tttgccgacg tgcagaatgg cgaggaacgc
     5401 ttcctgcggc agctcgacgt taccgatctg cttcatgcgt ttcttacctt ctttctgctt
     5461 ctgcagcagc tttttcttac ggctgatatc gccgccataa catttagcca gtacgttttt
     5521 acgcagctgt ttcacggtgg atcgcgcaat aatgtgcgta ccaatcgctg cctgaatggc
     5581 gatatcaaac tgctggcgtg ggatcagatc tttcatcttc tccaccaact cgcgaccgcg
     5641 gttttgcgaa ttatcacggt gggtgatcaa cgccagcgca tcaacacgtt caccgttgat
     5701 taatacgtct acacgtacca tgtcggacgc ctggaagcgt ttgaagttgt aatccagaga
     5761 cgcataacca cgcgaggtag atttcaggcg atcgaagaag tcgagcacca cttccgccat
     5821 cgggatctcg tacgtcagcg ccacctgatt accgtggtaa accatattgg tctgcacgcc
     5881 gcgtttttct acgcacagcg taataacgtt gccgagatat gcctgcggca gcagcatgtg
     5941 acactctgca atcggctcgc gcagttcgta aatgttattt accgcaggca gcttggatgg
     6001 gctgtcgacg tagataactt cccttgacgt ggtttcaact tcatacacta cggtcggcgc
     6061 agtggtgatc agatccagat cgtattcacg ttccagacgc tcctggatga tctccatgtg
     6121 cagcaggcca aggaagccgc agcggaaacc aaagcccagc gcgctggagc tttccggctc
     6181 atagaacagt gaggcatcgt tcaggctgag tttacccagc gcgtcacgga aggcttcata
     6241 gtcgtcggaa cttaccggga acagaccggc gtatacctgc ggtttgactt tcttaaagcc
     6301 aggcagcgcc ttttctgccg gattacgcgc cagcgttaag gtatcgccga ctggagcgcc
     6361 gtggatatct ttaatcgcac atacgagcca gcctacttcg ccacatttca gttcagtgcg
     6421 gtcaacctgt ttcggcgtga agatgcccag acggtcggcg ttataggtct gcccggtact
     6481 catgactttc actttgtcgc ccttacgcag ggtgccgttt ttaatacgga taagtgaaac
     6541 aacgcccagg tagttgtcga accatgagtc gataattagt gcctgcaacg ggccttccgg
     6601 atcgccttcc ggcggcggaa tgtcgcgcac cagacgttcg agaacgtcct gcacgccaac
     6661 gccggttttc gctgaacagc gcaccgcgtc agtggcgtcg atgccgacga tatcttcaat
     6721 ttcttccgcc acgcgttcag gatcggctgc cggcaggtca atcttgttca gtaccggcac
     6781 cacttcgaga tccatttcca tggcggtgta gcagtttgcc agggtttgcg cttccacgcc
     6841 ctgcccggcg tcgaccacca gcaatgcacc ttcacaggca gccagcgaac gggaaacttc
     6901 ataggagaag tctacgtggc ccggggtgtc gataaagtta agctgatagg tttcgccgtc
     6961 agacgctttg tagtccagcg tcacgctttg cgctttgatg gtaatgccac gctcacgctc
     7021 aagatccatg gaatcgagaa cctgcgcctc catttcacgg tcagacaggc caccgcagat
     7081 ctggataata cggtcagaca gcgtcgattt accgtggtca atgtgagcta tgatcgaaaa
     7141 gttacgtata ttcttcataa agtatgatta ttgtgcctta atgcccggtt aaccaggctt
     7201 ttagaagtcg ctgttctgag cttaacgtct gtattaatag aaacgccgca ttctacacta
     7261 caacattgag gcgaggaaat gttcataccg tatggattgt ggtatctgga aacgtcctcg
     7321 catttgttat gcaaaatgca acaaagccag tgaaatcact ggctcgcgtc ttccgaagat
     7381 gtttcaaatc gcacaaggcc aggcggcaat gccacgctta agatgatcgg ttgccattct
     7441 gcccgggcag caaacttacg cgagtagcca cgcgcaatca ggaacccgcc aataccgccg
     7501 aggaccgcac cacataatgc tgcgacgtca gaagcaaaga gtagctgaaa tagcgaagcg
     7561 atgaggaata atcccaccag cggcgacata taaaccagta atgcggagct aagcaggctg
     7621 ccttcggcga tccccaattc cactttttgc cccggcacta acggctcatc acagggtacg
     7681 acaatggtat gcgtggtttg cgggccaagt ttattcaaca cgcggctacc gcaaccggcg
     7741 cgtgaagcgc agctgctgca tgaggcttta acatcacaac tgaccagcgc ctgcccgttt
     7801 tgccaggaga cgacggtagc ccactctttg atcattgcgc tgccccgaac ttaatattct
     7861 cggcaatgcg tttcgccgtt tgcggcggca gttcaccgac aatggtgatt tcggcgttat
     7921 cacgtacgct tgtactgacg gttctgcgtc cggtgcgcaa catctgatcg gtgctcgatg
     7981 gcgtagcgcg gttaacgttt accgagaagc tgaataatcc gtcggaatag agacgtgatt
     8041 cgataggcat gttgtccatc gtcggtagcg gacgtcgact actggaaact tcgctaaaac
     8101 cctgtggcaa ccaggttggc gtccagctga atttagcttt ttcacctaca ggaacagaaa
     8161 gcaacggcgg caaatttgcc ttcgccagcg tctgcatact gctgctgata tcctgattga
     8221 cgttaaaagc aatcacgcga aattgttcca gcgtttcacc atcgcgatca agaagatcaa
     8281 cccgcatcgg taatttcgat tcggtgtcca tccacacgat gtagctgtag cgtgtaccat
     8341 ctcgggcaac cacgcgaatg acttcgcaaa gacgatcagc aatacgcgtg cgcccgacgg
     8401 agataaagtc gtagtaagga gaaaggcgtt tgaaatcggt atagatgagc gatggcagag
     8461 aatcaacaat gtaatcgcca ttaagcgtga acggttcaag tcccggttca aaatagctga
     8521 tttcattgcc gcgctgtacc acttcccggc gcgggccatc catttgcaac aattgtgcaa
     8581 gaggacggtt atcgaggcgt gcatgtcgat aacgcagaga ctcaacaccc tgtttattga
     8641 tgctgatgaa tgacagctcg taattcagtg actgactggc caggttcatc tgctgtaata
     8701 acgccccgga cgcgggagtg gccgaggcgt tagcagagaa taacaggcta cctgtcacta
     8761 atgacatggc aaaccaaagt tgcttcatta ctgcgattgc gttcctaaag tttgaattcc
     8821 tggcacctgt acagcggctt gctgggtttg cgcctgctca aactgaagct gttcagagtg
     8881 gagtcggcgt tgcagttcgt aatcctgcaa cattgcatta atgcgacgac gctgctgctg
     8941 ttgaccatta tttgcggtcg cttcagaagg tactcccagg ctaaccgggc tggctttacc
     9001 catcatcggc agtgtattaa ataccggcgt ttcgggctgc tgggacgttt cagattgtcc
     9061 attatagtgc tggacgccaa cgataactgc aagcgatacg catgcggcta cgcccatttg
     9121 ggtaagctgt gccgcccacg gacgtacttt ctgccagaat ggcattttct gccattcatg
     9181 cggcgcaggc tgggcttccg ggatcaatgt cgccggttga cgtactggct cttcttcaat
     9241 ggcggccatc acgcgtgaag agatatcgaa atggagcacc tcgggagtat caccccgcat
     9301 tgagtcacgg attaagtgat agctttccca ggttttctgc atttctgggt tatgagccag
     9361 ttcgttaagc agctcactat ccagcgtttc gccatccatt aaagcggaaa gttgttcttt
     9421 ctgcatgcct aataccctta tccagtatcc cgctatcgtc aacgcctgat aagcggttga
     9481 actttgttat caatagcttc cctcgctcgg aagatacgtg aacgcaccgt acctaccgga
     9541 caatccatga tagcggctat ctcttcatag ctcaggccat ccagctcccg caaggttatt
     9601 gccatgcgta aatcttccgg gagggactca atagttcgga aaactatctg tctcagttct
     9661 tctgacaaca ttaagttctc agggttcgaa atttctttca acgcgccgcc actttcgaag
     9721 ttttcagctt caatggcatc cacatcactg gaaggtggac gacgcccctg agcaaccagg
     9781 taatttttcg ctgtatttac agcaatccga tacagccatg tataaaaagc gctatctccc
     9841 cggaacgaat ccagcgcacg ataggcttta ataaaagctt cttgtaccac atcgggaaca
     9901 tcacccgacg gcacatagcg ggaaaccaga ctcgccactt tatgctgata gcgcactacc
     9961 agtaagttaa aggctttctg atctcccttc tggacccgtt caaccaggac ctggtccgtt
    10021 aactgctcgc tcatccgagg taaagtctcc ccaaaccaaa tttccacgcg ctatcgaaac
    10081 gccactccat tagctgcaat ttgagcaagc aaagggttag agtgtctcgt ttttgtaaag
    10141 ttccgtaacg catctgtttt tgtttgtcat gctgtagacg gatcattatc tatcattata
    10201 agtctacaga atctgaacat cgcattatct gtgtagaaat gcccatttaa ctgcctgaag
    10261 agtaacccaa cggccttttt atttcaccac ctaatcctcc accagccagt aacttctctt
    10321 tttctcgccg ccctgcgtca gcgtgtttag tcactgtaac aaatattaaa atagcaggtg
    10381 tttattcgca caacatgatg ctatgctgac caaaccgtgt ttagtaaatt aaacaaagaa
    10441 aatgaatact ctccttgaac attcatgtga cgtgttgatt atcggtagcg gcgcagccgg
    10501 gctttcactg gcgctacgcc tggctgacca gcatcaggtc atcgttctaa gtaaaggccc
    10561 ggtaacggaa ggttcaacat tttatgccca gggcggtatt gccgccgtgt ttgatgaaac
    10621 tgacagcatt gactcgcatg tggaagacac attgattgcc ggggctggta tttgcgatcg
    10681 ccatgcagtt gaatttgtcg ccagcaatgc acgatcctgt gtgcaatggc taatcgacca
    10741 gggggtgtta tttgataccc acattcagcc gaatggcgaa gaaagctacc atctgacccg
    10801 tgaaggtgga catagtcacc gtcgtattct tcatgccgcc gacgcaactg gtagagaagt
    10861 agaaaccacg ctggtgagca aggcgctgaa ccatccgaat attcgcgtgc tggagcgcag
    10921 caacgcggtt gatctgatta tttctgacaa aattggcctg ccgggcacgc gacgggttgt
    10981 tggcgcgtgg gtatggaacc gaaataaaga aaaggtggaa acctgccacg caaaagcggt
    11041 ggtgctggca accggcggtg cgtcgaaggt ttatcagtac accaccaatc cggatatttc
    11101 ttctggcgat ggcattgcta tggcgtggcg cgcaggctgc cgggttgcca atctcgaatt
    11161 taatcagttc caccctaccg cgctgtatca cccacaggca cgcaatttcc tgttaacgga
    11221 agcactgcgc ggcgaaggcg cttatctcaa gcgcccggat ggcacgcgtt ttatgcccga
    11281 ttttgatgtg cgcggcgaac tggccccgcg cgatattgtc gcccgcgcca ttgaccatga
    11341 aatgaaacgc ctcggcgcag attgtatgtt ccttgatatc agccataagc ccgccgattt
    11401 tattcgccag cattttccga tgatttatga aaagctgctc gggctgggga ttgatctcac
    11461 acaagaaccg gtaccgattg tgcctgctgc acattatacc tgcggtggtg taatggttga
    11521 tgatcatggg cgtacggacg tcgagggctt gtatgccatt ggcgaggtga gttataccgg
    11581 cttacacggc gctaaccgca tggcctcgaa ttcattgctg gagtgtctgg tctatggctg
    11641 gtcggcggcg gaagatatca ccagacgtat gccttatgcc cacgacatca gtacgttacc
    11701 gccgtgggat gaaagccgcg ttgagaaccc tgacgaacgg gtaataattc agcataactg
    11761 gcacgagcta cgtctgttta tgtgggatta cgttggcatt gtgcgcacaa cgaagcgcct
    11821 ggaacgcgcc ctgcggcgga taaccatgct ccaacaagaa atagacgaat attacgccca
    11881 tttccgcgtc tcaaataatt tgctggagct gcgtaatctg gtacaggttg ccgagttgat
    11941 tgttcgctgt gcaatgatgc gtaaagagag tcgggggttg catttcacgc tggattatcc
    12001 ggaactgctc acccattccg gtccgtcgat cctttctccc ggcaatcatt acataaacag
    12061 ataaaaagcc tgggttagcg ccgtatacgc ttcggaatag ttctggtctg gcccacgaat
    12121 gactaagcga tcgctaaagc attctcccgc ctgcggggag aatgccagca gcacccgatg
    12181 cggcagtcgc gcttcgtttt ccgccacatc cgtccgcaaa cgtaaatgcc agcccatact
    12241 taatgccagc tccgtaaaac tattaccaat ctgctctggc agcaccacgc agaaaaatcc
    12301 ctcttcggta atgcactccg ccgcgcaagt aagtaaagtc tggtgatcaa gtgaggtggt
    12361 gtagcgcgcc tgttcccgct gcggcgtcgc gcactccact ccttgctcat aatacggcgg
    12421 gttgctgatg attaaatcaa agcgcgcggt ctgctgtgtt acccactgct gaatatccgc
    12481 cgtatggacg ttaattctct ctaaccacgg ggactgggcg acattttcct gcgcctgagt
    12541 ggcagcttca ctttccagct caacggcatc aattatcacg ctgtcactgg ttcgctgcgc
    12601 cagcatcagt gccagcaagc cactacccgc accgatatca aggcaacgtt ttaccccagc
    12661 caccggtgcc catgcgccca gcaatatgcc atccgttccc actttcatcg cacagcgatc
    12721 gtgagcaaca aaaaactgtt taaaagtaaa tccattacga cgaagcacgg atgtagactg
    12781 tgacatgaaa ataaaacctt gcaggaaaaa cggcgatagc accgggtgag aacaataccc
    12841 gagaagcgat atccatacaa acagatgaag attgcagccg taacgtctat aatcagcgcc
    12901 ccacacagag gtagaacatg actgtaacga ctttttccga acttgaactc gacgaaagcc
    12961 tgctggaagc cctccaggat aaaggtttca ctcgcccgac cgccattcag gctgccgcca
    13021 ttccgcctgc gctcgatggc cgtgatgtac tcggttctgc gccgacaggc accggtaaaa
    13081 cggcggcgta tctgctgcca gcgttgcagc acctgctcga tttcccgcgt aagaaatccg
    13141 gtccgccgcg tattttgatc ctcaccccaa ctcgcgagct ggcgatgcag gtggccgatc
    13201 atgcccgcga actggcgaaa catacgcatc tggatatcgc caccatcacc ggcggcgtag
    13261 cctatatgaa ccacgcggaa gtgttcagcg aaaatcagga catcgtggtc gccacgaccg
    13321 gacgtctgct gcaatacata aaagaagaga acttcgattg ccgcgcggtt gaaacgctga
    13381 tcctcgacga agcagaccgt atgctggata tgggcttcgc tcaggatatc gaacatattg
    13441 ctggcgaaac gcgctggcgt aaacagaccc tgctcttttc ggcaacgctg gaaggcgatg
    13501 cgattcagga ctttgccgag cgtctgctgg aagatccggt ggaggtttct gccaatccgt
    13561 ccacccgtga gcgcaaaaaa attcatcagt ggtattaccg cgccgatgac ctggaacata
    13621 aaaccgcatt gctggtgcat ctgttaaaac agccggaagc gacccgctca attgtgtttg
    13681 tgcgtaagcg tgagcgtgtg catgagctgg caaactggct gcgcgaagcg ggcatcaaca
    13741 actgctatct cgaaggtgag atggtacagg gcaagcgtaa cgaagcgatc aaacgtttga
    13801 ccgaaggtcg cgtaaacgta ctggttgcaa ccgatgttgc cgcgcgcggt atcgacattc
    13861 ctgacgtcag ccacgtcttt aacttcgata tgccgcgcag tggcgatact tatttgcacc
    13921 gtatcggacg taccgcgcgc gccggtcgta aaggcaccgc aatttcgctg gtagaagccc
    13981 atgaccatct gctgctgggc aaagttggtc gctatattga agagccaatt aaagctcgcg
    14041 taattgatga gttacgcccg aaaacgcgtg caccaagcga gaagcagacc ggcaagccat
    14101 cgaagaaagt actggcaaaa cgcgccgaga agaaaaaagc aaaagagaaa gagaagccgc
    14161 gggtgaaaaa acgccatcgc gataccaaaa atattggtaa gcgccgtaaa ccaagcggaa
    14221 cgggcgtgcc accgcaaacg acagaagagt aatctcaatg ccgggtttaa gcccggcatt
    14281 atttttatcc cggcaaaaaa caatcttcaa cacactgaat aaaagtgtgc atcgccgggc
    14341 taaccgcttt tccggcatgg tgagcgcaca ttgccgtaat ggtctgcgag tgttcgccaa
    14401 aaggcaattc aattaattct ccgctctcaa gctctttttc caccgcaaag cggggcaagt
    14461 aactgacacc gatattcgcc gcgacacaac gtttgatact ttcaatgctt aacagctcaa
    14521 tggtgttttc caccgtgatc cgccgctggc gcagcgtgct ctcaaatatc tgccggaata
    14581 cacattgcgg ttcgttgata ataaagctac aggcgttatg tcttcccggc tcagtaaaat
    14641 cgacatctgc aatttgcggt gaagctacca gcaccagcga ttgctcaccc aattctcgtc
    14701 ggttcagggc atcatcattc cctacgcgat aaaaaacccc gacatcggcc tcatcattca
    14761 atagcgcatc acggatcacg taacagttca gagactgcaa cgataaacgc acttttgggg
    14821 cgcgttgccg aaaacgctgc aacacctgcg gcattcggta agagagcaac gtttcgcccg
    14881 aaacaacgcg tagttccccg tccggatccg actccttttt ggcagcctca cgaagggtat
    14941 ccatcactct ggtgagttca taaatgtgcg gcagcagctt tttaccttcg cgagtgaggc
    15001 acatccgtcg gccaattttc tcaaataact gtaccgagaa ctcctgctca agctgctgaa
    15061 tatgaaaagt caccgtcgat tgtgtacagc acaatttttg cgaagctcgc aaaaaggaac
    15121 cctcttccac cacggtttta agcgtaataa aacggcgcag atccataacc ccaaacctat
    15181 cgaaaatatc gaatttagaa tgtaaaaaca ttcatttttt taaatattct gtgtcgggta
    15241 ctgtctacca aaacagagga gataacaagt gacaccgacc cttttaagtg ctttttggac
    15301 ttacaccctg attaccgcca tgacgccagg accgaacaat attctcgccc ttagctcagc
    15361 cacaacgcac ggatttcatc agagtacccg cgtgctggca gggatgagtc tgggattttt
    15421 aatcgtcatg ttgctgtgtg cgggcatttc gttttcactg gctgttatcg acccggcagc
    15481 tgtgcatctg ttgagttggg cgggggcggc atatattgtc tggctggcgt ggaaaatcgc
    15541 taccagtcca acaaaggaag acgggcttca gacaaaacca atcagttttt gggccagctt
    15601 tgctttgcag tttgtgaacg tcaaaatcat tttgtacggt gttacggcac tgtcgacatt
    15661 tgttctgccg caaacacagg cgttaagctg gatagttggc gtcagcgttt tgctggcaat
    15721 gattgggacg tttggtaatg tgtgctgggc gctggcgggg catctgtttc agcgattgtt
    15781 tcgccagtat ggtcgccagt taaatatcgt gctggcgctg ctgctgatct attgcgcggt
    15841 acgcattttc tattaatgaa aaaaagcgga agaggtcgcc ctcttccgct tagtaacatg
    15901 ctacttaagc cttacaggct ttcagtaaag gtacgagcga taacgtcgcg ctgctgttcc
    15961 ggagtcagag agttaaagcg aactgcataa ccggatacac ggatggtcag ctgcggatat
    16021 ttttccggat gcttaactgc atcttccaga gtttcacgac gcagaacgtt aacgttcagg
    16081 tgctgaccac cttcaacgcg aacttctggt ttcacttcta ctggaacttc acggtactca
    16141 atgtcaccca gtttgcttac tgcaaccact tcgtcttctg cataacctgc tttcgcaacg
    16201 atgcaacgcg cttcgccttt ttcgctgtcc agcagccaga aagagttcag cagatcgtcg
    16261 ttagcggctt tagtaatctg gatacctgta atcatgtgat gcctccccgg caaaattatt
    16321 tgatttgttc agcctgtcgc ggccaattgg taaaaccatt gttgcttgag tgtatatata
    16381 ctcctcaaac acccttgaat ctttgattta aatcaataaa aaccacacat caagtgtggt
    16441 cgcaaataga ttttattgtt ttacatcaac ttatgcgggt gtgaaatttt accaatttac
    16501 atttttttgc actcgtttaa gtctaaaaaa tgagcatgat tttgttctgt agaaagaagc
    16561 agttaagcta ggcggattga agattcgcag gagagcgaga tggctaacga attaacctgg
    16621 catgacgtgc tggctgaaga gaagcagcaa ccctattttc ttaataccct tcagaccgtc
    16681 gccagcgagc ggcagtccgg cgtcactatc tatccaccac aaaaagatgt ctttaacgcg
    16741 ttccgcttta ccgagttggg tgacgttaaa gtggtgattc tcggccagga tccttatcac
    16801 ggaccgggac aggcgcatgg gctggcattt tccgttcgcc ccggaatcgc cactcctccg
    16861 tcactgctga atatgtataa agagctggaa aatactattc cgggcttcac ccgccctaat
    16921 catggttatc ttgaaagctg ggcgcgtcag ggcgttctgc tactcaatac tgtgttgacg
    16981 gtacgcgcag gtcaggcgca ttcccacgcc agcctcggct gggaaacctt caccgataaa
    17041 gtgatcagcc tgattaacca gcatcgcgaa ggcgtggtgt ttttgttgtg gggatcgcat
    17101 gcgcaaaaga aaggggcgat tatagataag caacgccatc atgtactgaa agcaccgcat
    17161 ccgtcgccgc tttcggctca tcgtggattc tttggctgca accattttgt gctggcaaat
    17221 cagtggctgg aacaacatgg cgagacgccg attgactgga tgccagtatt accggcagag
    17281 agtgagtaaa tttgcgggaa aatgccggat ggcagagttg ccacccggct gatttatcag
    17341 gctttattct gacgccacca ttcgccaaga agaacgccgg ttgcgacaga gatattcagc
    17401 ccggcaacgt tgccagtacc atcaatcttc acgcgcagat cgttcggatc gcgtgcggca
    17461 tccggtaacc cttcatattc ctgacccagc accagtacca ttttcgctgg cagactggtt
    17521 ttgaacagcg gtttaccctg ctcgctggaa gttgtcacta cggtgtaacc cgcctgacgg
    17581 aaatcatcca gcacgttaac aatgttgtcg ccagtaatcg gctgaacgtg ctctgcgccg
    17641 ccttctgcgg tacggatagc cgcccccgac tccagcagtg ccgcatcctg caccacaaca
    17701 cctttcacgc caaagtgcgc gcagctgcgc atcatgccac ccaggttatg cgggttagat
    17761 tcgttttcca gtgccagaac acaatcctgc gcgcctgcct ggcttaccca ctgctgcacg
    17821 gttgtgccat tacgcttttt gatcaggaag caaacgccac cgtgatgttc cgtgcctgac
    17881 gcttttgtca attccgcttc atctaccaca tggtacgctt tgcggtttgc tgccatccag
    17941 cgcaaggctt ctttaaaacg cggcgttaca ctctggataa accaggcgcg aacaatcgct
    18001 tccggacggc tctggaacag tgcctgacag gcgttttcgc cgtagacgcg ggtttcttcc
    18061 gcacgctgac gacgcaacac ttccggatca ataaaacttt taccactgat gccaccgtga
    18121 tcggcctttt ccggcgtctc atcacccggc gcgcgggaaa ccgtgcgcca cggcgaatct
    18181 tcccacttgc ggtcacgggg ctgactctgt ttgtcatcgc gggcggggcg acggccaccg
    18241 tcagcacgag attttcctgg acgcccgccc cctttcccgg tacgcgggtt gtgggtacgt
    18301 ttatcagaat catcatcact gcggacatac atcactttga ccttgccgct tttacctttc
    18361 atttcatcgt tcatgctttt ctccaccagc gctgcgcgaa gcgcgcagat tacccgaagt
    18421 ccgcgcggtt cgccatgatt tcgtaccaaa gcctgcgact atcataccta ttgaataaaa
    18481 cagattgttg tctggaacaa tgtccccgat aatatgtaac atattagaaa cataccggcg
    18541 ttgttgccga taagtctcct tactcatccc gaggttagtt atgaataccg tttgtaccca
    18601 ttgtcaggcc atcaatcgca ttcccgacga tcggatcgaa gatgcggcaa aatgcggacg
    18661 ctgcggtcac gacttgtttg acggagaggt gattaatgcg accggtgaaa cgctcgacaa
    18721 attgctgaaa gatgatctac cagtggtgat cgacttctgg gcaccgtggt gcggcccctg
    18781 ccgtaatttc gcaccaattt ttgaagatgt cgcgcaagag cgtagcggta aagtgcgctt
    18841 tgtgaaagtg aataccgaag ctgaacgtga attgagcagt cgctttggaa ttcgtagtat
    18901 accgacgatc atgattttca aaaacggtca ggttgtcgac atgcttaatg gcgcagtacc
    18961 gaaagcgccg ttcgatagct ggctgaacga atctctttaa tcttaccggg gcgcatcttg
    19021 tgccccgttt tctcctctgc gacaatggcg ttttttcgac gctctcttat gaccgaaaac
    19081 gctgttctcc agttacgcgc cgagcgtatt gcgcgcgcaa cacgtccttt tcttgcccgc
    19141 ggtaatcgcg ttcgtcgctg ccaacgctgt cttttgccag agaaattatg tctctgttcg
    19201 acaattaccc cagcacaagc aaaaagtcgc ttctgtttgc tgatgttcga caccgagcca
    19261 atgaagccca gtaataccgg gcgtctgatt gctgatattt tgcctgatac cgttgcgttt
    19321 caatggtcgc gtaccgaacc ctcgcaggat ttgctggatc tggtacaaaa cccggattat
    19381 cagccaatgg tggtctttcc cgcctcgtat gctgatgagc aacgggaagt gatcttcaca
    19441 ccacctgccg gtaagccacc gctgtttatc atgctcgatg gtacctggcc ggaagctcgc
    19501 aagatgtttc gtaaaagtcc gtatctggat aatcttcccg tcatttccgt cgatctttcc
    19561 cggctttctg cctatcgcct gcgtgaagcc caggctgaag gccaatattg tactgccgag
    19621 gtagccatcg cactgttaga tatggccggc gataccgggg cggcagcagg gttaggcgag
    19681 cacttcaccc gctttaaaac gcgctatctg gcaggaaaaa cgcaacatct gggtagcatc
    19741 acagcagaac agttagaaag cgtttaaaat cattcggtca cttctgcggg agaccggtat
    19801 gagtcagcga ggactggaag cactactgcg accaaaatcg atagcggtaa ttggcgcgtc
    19861 gatgaaaccc aatcgcgcag gttacctgat gatgcgtaac ctgctggcgg gaggctttaa
    19921 cggaccggta ctcccggtga cgccagcctg gaaagcggtg ttgggtgtgt tggcctggcc
    19981 ggatattgcc agcttgccct ttacacccga ccttgcggtt ttatgtacca atgccagccg
    20041 taatcttgct cttctggaag agctcggcga gaaaggctgt aaaacctgca ttattctttc
    20101 cgccccggca tcgcaacacg aagatctccg cgcctgcgcc ctgcgccata acatgcgcct
    20161 gcttggacca aacagtctgg gtttactggc tccctggcaa ggtctgaatg ccagcttttc
    20221 gcctgtgccg attaaacgcg gcaagctggc gtttatttca cagtcggctg ccgtctccaa
    20281 caccatcctc gactgggcgc aacagcgtga gatgggcttt tcctacttta ttgcgctcgg
    20341 cgacagccta gatatcgacg ttgatgaatt gcttgactat ctggcacgcg acagtaaaac
    20401 cagcgccatc ctgctctatc tcgaacagtt aagcgacgcg cgacgctttg tttcggcggc
    20461 ccgtagtgcc tcgcgtaata aaccgattct ggtgattaaa agcggacgta gcccggcagc
    20521 acagcgactg ctcaacacga cggcaggaat ggatccggcg tgggatgcgg cgattcagcg
    20581 tgccggtttg ttgcgggtgc aggataccca cgagctgttt tcggcggtgg aaaccctcag
    20641 ccatatgcgc ccgctgcgtg gcgaccggtt gatgattatc agcaacggtg ctgcgcctgc
    20701 cgcgctggcg ctggatgcct tatggtcacg caatggcaag ctggcaacgc taagcgaaga
    20761 aacctgccag aaactgcgcg atgcactgcc aggacatgtg gcaatctcta acccgctcga
    20821 tctgcgcgat gacgccagca gcgagcatta tgtcaaaacg ctggacattc tgctccacag
    20881 ccaggatttt gatgcgctga tggttattca ttcgcccagc gccgctgctc ccgcaacaga
    20941 aatcgcgcaa gtattaattg aagcggtaaa gcatcatccc cgcagcaagt atgtttctct
    21001 gctgacgaac tggtgcggcg agcactcctc gcaagaggca cgacgtttat tcagcgaagc
    21061 cgggctgccg acctaccgta ccccggaagg aaccatcact gcttttatgc atatggtgga
    21121 gtaccggcgt aatcagaagc aactacgcga aacgccggcg ttgcccagca atctgacttc
    21181 caataccgca aaagcgcatc ttctgttgca acaggcgatt gccgaagggg ctacgtcgct
    21241 cgatacccat gaagttcagc ccatcctgca atcgtatggg atgaacacgc tccctacctg
    21301 gattgccagc gatagcaccg aagcggtgca tattgccgaa cagattggtt atccggtggc
    21361 gctgaaattg cgttcgccgg atattccaca taaatcggaa gttcagggcg tcatgctgta
    21421 cctgcgtaca gccaatgaag tccagcaagc ggcaaacgct attttcgatc gcgtaaaaat
    21481 gacctggccg caggcgcggg tccacggcct gttggtgcaa agtatggcta accgtgctgg
    21541 cgctcaggag ttgcgggttg tggttgagca cgatccggtt tttgggccgt tgatcatgct
    21601 gggtgaaggc ggtgtggagt ggcgtcctga agatcaagcc gtcgtcgcac tgccgccgct
    21661 gaatatgaac ctggcccgct atctggttat tcaggggatc aaaagtaaaa agattcgtgc
    21721 gcgcagtgcg ctacgcccat tggatgttgc aggcttgagc cagcttctgg tgcaggtttc
    21781 caacttgatt gtcgattgcc cggaaattca gcgtctggat attcatcctt tgctggcttc
    21841 tggcagtgaa tttaccgcgc tggatgtcac tctggatatc gcgccgtttg aaggcgataa
    21901 cgagagtcgg ctggcagtgc gcccttatcc gcatcagctg gaagaatggg tagaattgaa
    21961 aaacggtgaa cgctgcttgt tccgcccgat tttgccagaa gatgagccac aacttcagca
    22021 attcatttcg cgggtcacca aagaagatct ttattaccgc tactttagcg agatcaacga
    22081 atttacccat gaagatttag ccaacatgac gcagatcgac tacgatcggg aaatggcgtt
    22141 tgtagcggta cgacgaattg atcaaacgga agagatcctc ggcgtcacgc gtgcgatctc
    22201 cgaccctgat aacatcgatg ccgaatttgc cgtgctggtt cgctcggatc tcaaagggtt
    22261 aggcttgggt cgacgcttaa tggaaaagtt gattacctat acgcgagatc acggactaca
    22321 acgtctgaat ggtattacga tgccaaacaa tcgtggcatg gtggcgctgg cccgcaagct
    22381 cgggtttaac gttgatatcc agctcgaaga ggggatcgtt gggcttacgc taaatcttgc
    22441 ccaacgcgag gaatcatgag taaggtactg gaaatgttga ccacttaatc gggactggtg
    22501 ttattattgc acgcttatgt cgtctgcatt gcacagagga cccttcaatg aacagagaag
    22561 aaatgcactg tgatgttgtc aaaatttaag cgtaataaac atcaacaaca ccttgcccaa
    22621 ctacccaaga tttctcaatc agttgatgat gtcgatttct tttacgctcc cgccgacttc
    22681 cgggagacgc tgctggaaaa aatagccagc gcgaagcagc gcatttgcat tgtcgccctg
    22741 tatctcgaac aggatgacgg tggcaaaggc attctgaacg cgttgtatga agctaaaagg
    22801 cagcgtccgg aactggatgt gcgggtgctg gtcgactggc atcgtgcaca acgtggacgc
    22861 attggcgctg cggcatctaa cactaacgct gactggtact gtcgcatggc gcaggaaaat
    22921 ccgggcgtag atgttccggt ttatggcgtt ccaatcaata cccgtgaagc ccttggtgtt
    22981 ctgcacttta aaggctttat catcgacgat agcgtacttt atagcggtgc cagcctgaac
    23041 gatgtttacc tgcatcagca cgataaatat cgctacgacc gttatcatct tatccgtaac
    23101 cgtaagatgt cagacattat gtttgaatgg gttacacaga atattatgaa tggccgcggc
    23161 gttaatcgtc tggatgatgt taatcggcca aaaagcccgg aaatcaagaa cgatattcgt
    23221 ctgttccgcc aggagctgcg tgatgccgct tatcatttcc agggcgatgc cgacaacgat
    23281 cagctttccg taacgccgct agtggggctg gggaaatcga gtctgttgaa caagaccatt
    23341 ttccatctta tgccttgtgc cgagcagaaa ctaaccatct gtacgccata cttcaacctg
    23401 ccagcaatcc ttgtgcgcaa tattatccag ttgctgcgcg aagggaaaaa ggtcgaaatt
    23461 attgttggtg ataaaaccgc gaacgacttc tacattccgg aagatgaacc tttcaagata
    23521 attggcgcat tgccttatct ctatgagatc aatctgcgtc gtttcctgag ccgtttgcag
    23581 tattacgtca atactgacca gctagtggtt cggttatgga aagatgacga caacacctat
    23641 cacctaaaag ggatgtgggt tgatgataag tggatgttga tcaccggtaa taacctgaac
    23701 ccgcgcgcct ggcgtctgga tctggaaaac gccattttga tccacgatcc acaactggag
    23761 cttgcgccgc agcgagagaa agaactggag ctgatccgcg agcataccac catcgttaag
    23821 cactatcgcg atctgcaaag tattgccgat tatccggtga aggttcgtaa actcatccgc
    23881 cgtttgcgcc gtatccgcat cgaccgatta attagccgca tcctgtaatc acaaccccgt
    23941 cctgtacggg gtttgttttt tggaggccac gttttgcgta ttctttttgt ctgttcgcta
    24001 ttgttacttt ctggatgcag ccatatggct aacgatagct ggagcgggca ggataaagct
    24061 caacacttta tcgcctcggc gatgctttcc gccgccggaa atgaatattc acagcatcag
    24121 gggatgagcc gggatcgcag tgccatgttt ggtttgatgt tctctgtcag tttgggggcg
    24181 tcaaaagagt tttgggatag ccgccccgaa gggagcggct ggagctggaa ggatttggcc
    24241 tgggatgtcg ccggtgcaag caccggctat accgtctggc aactgacccg tcactaaaga
    24301 cgcatcccct tccctttgcg atgtagcatc aaagaaacca gaaacgccac cacggccatc
    24361 aaggtcacat accagaagaa ggctgtttcc attcctattg atttcagcga caacgctacg
    24421 tactccgccg aaccaccaaa tatagcatta gcgaccgcat atgacagacc aacgcctaat
    24481 gcgcgaacct gtgccgggaa catctcagcc ttcagtattc cactgattga tgtataaaaa
    24541 ctcactatca gcagggcaca catcaccaga ccaaaagcgg cataaggcga ggaaacgttt
    24601 tgcaatgctg agagaatagg aacggtaaaa atggctgcca gcgaaccgaa acataacatt
    24661 gaggtacggc gaccaatctt atccgacagc gcgccaatga gtggttgaat aagcatgaat
    24721 acaaacaatg cggcagtcat aatgccactc gccacgttgg catgcattcc cgcagtattt
    24781 accagatact tctgcatata agtggtgaag gtatagaaac aaagggagcc cgcagcggta
    24841 aaaccgagaa ccatgatgaa tgcacggcga ttgcgccata atcctttcag agatccagct
    24901 tcttttaaag cgcgcgtttc ttgttgcgaa gtttcatcta actgacgacg taaccacaac
    24961 gccacaacag ctaacacagc tcctaacgcg aaaggaatac gccatcccca ctctctgagt
    25021 gcagagtctt ccatggtgtg ttgtaaaacc acgacaacca gtagggctag cagttgtccg
    25081 ccgatcaacg tcacatactg aaatgatgcg taaaaacctt tgcgcccttc aacggcaact
    25141 tcactcatat aggtggcgct ggtgccatat tctccgccaa cggataatcc ctgaaataaa
    25201 cgcgcgagaa gcaataatgc cggagcccac gtaccgattg tttcataacc aggcaaacag
    25261 gcgattacca gtgagccgaa acacatcata cacaccgata gcagcatcga ttttttgcga
    25321 ccatgtttat cggctatacg gccaaatagc caaccgccta ttgggcgcat caggaatccc
    25381 gcagcaaaaa cacctgctgt ttgtagtagt tgagtcgtcg tgttcccgga agggaagaag
    25441 atgtgggcaa agtagagtga acagaacgag tagacataga aatcgaacca ctcgaccaga
    25501 ttacctgaag aggcccccac aatcgcccaa atgcggcgac gagtatcact acttgtcagt
    25561 ttgctgtctg ccgttacagt actttcagcc atgccattat gtctcctgcc gtaatccgat
    25621 gcttttgtcg gtcgcttttg tttatttctt ttggcaaaga aaatgttata catttgttac
    25681 gtatcattat gcaaccttaa ccatgaattt agttagcagg aaaatggtta ttgaggagct
    25741 taaggataaa tttctggtaa ggaggacacg tatggaagta ggcaagttgg ggaaaccgta
    25801 tccgttgctg aatctggcat atgtgggagt ataagacgcg cagcgtcgca tcaggcattt
    25861 ttttcaccaa cgcaaaaagg ccatcctgac ggatggcctt t
//
LOCUS       DAZFDC010000062        25513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFDC010000062 DAZFDC010000000
VERSION     DAZFDC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.062"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            441..1580
                     /gene="wza"
                     /locus_tag="ACHJ48_002649"
     CDS             441..1580
                     /gene="wza"
                     /locus_tag="ACHJ48_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO4708183.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1586..2029
                     /gene="wzb"
                     /locus_tag="ACHJ48_002650"
     CDS             1586..2029
                     /gene="wzb"
                     /locus_tag="ACHJ48_002650"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO4708184.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2032..4194
                     /gene="wzc"
                     /locus_tag="ACHJ48_002651"
     CDS             2032..4194
                     /gene="wzc"
                     /locus_tag="ACHJ48_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO4708185.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4287..5126
                     /gene="wcaA"
                     /locus_tag="ACHJ48_002652"
     CDS             4287..5126
                     /gene="wcaA"
                     /locus_tag="ACHJ48_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO4708186.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5129..5617
                     /gene="wcaB"
                     /locus_tag="ACHJ48_002653"
     CDS             5129..5617
                     /gene="wcaB"
                     /locus_tag="ACHJ48_002653"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO4708187.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5614..6831
                     /gene="wcaC"
                     /locus_tag="ACHJ48_002654"
     CDS             5614..6831
                     /gene="wcaC"
                     /locus_tag="ACHJ48_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO4708188.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6806..8023
                     /gene="wcaD"
                     /locus_tag="ACHJ48_002655"
     CDS             6806..8023
                     /gene="wcaD"
                     /locus_tag="ACHJ48_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO4708189.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKITQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGICAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8034..8780
                     /gene="wcaE"
                     /locus_tag="ACHJ48_002656"
     CDS             8034..8780
                     /gene="wcaE"
                     /locus_tag="ACHJ48_002656"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO4708190.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8796..9344
                     /gene="wcaF"
                     /locus_tag="ACHJ48_002657"
     CDS             8796..9344
                     /gene="wcaF"
                     /locus_tag="ACHJ48_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO4708191.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9373..10494
                     /gene="gmd"
                     /locus_tag="ACHJ48_002658"
     CDS             9373..10494
                     /gene="gmd"
                     /locus_tag="ACHJ48_002658"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO4708192.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10497..11462
                     /gene="fcl"
                     /locus_tag="ACHJ48_002659"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10497..11462
                     /gene="fcl"
                     /locus_tag="ACHJ48_002659"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO4708193.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11465..11944
                     /gene="gmm"
                     /locus_tag="ACHJ48_002660"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11465..11944
                     /gene="gmm"
                     /locus_tag="ACHJ48_002660"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO4708194.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            11941..13164
                     /gene="wcaI"
                     /locus_tag="ACHJ48_002661"
     CDS             11941..13164
                     /gene="wcaI"
                     /locus_tag="ACHJ48_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO4708195.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13167..14603
                     /gene="cpsB"
                     /locus_tag="ACHJ48_002662"
     CDS             13167..14603
                     /gene="cpsB"
                     /locus_tag="ACHJ48_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO4708196.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14796..16166
                     /gene="cpsG"
                     /locus_tag="ACHJ48_002663"
                     /gene_synonym="manB"
     CDS             14796..16166
                     /gene="cpsG"
                     /locus_tag="ACHJ48_002663"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO4708197.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDARLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPSSGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16221..17615
                     /gene="wcaJ"
                     /locus_tag="ACHJ48_002664"
     CDS             16221..17615
                     /gene="wcaJ"
                     /locus_tag="ACHJ48_002664"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO4708198.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKLGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17617..19095
                     /gene="wzxC"
                     /locus_tag="ACHJ48_002665"
     CDS             17617..19095
                     /gene="wzxC"
                     /locus_tag="ACHJ48_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO4708199.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19275..20555
                     /gene="wcaK"
                     /locus_tag="ACHJ48_002666"
     CDS             19275..20555
                     /gene="wcaK"
                     /locus_tag="ACHJ48_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO4708200.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQVYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGVMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20552..21772
                     /gene="wcaL"
                     /locus_tag="ACHJ48_002667"
     CDS             20552..21772
                     /gene="wcaL"
                     /locus_tag="ACHJ48_002667"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO4708201.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THVAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESW
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIHGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21783..23177
                     /gene="wcaM"
                     /locus_tag="ACHJ48_002668"
     CDS             21783..23177
                     /gene="wcaM"
                     /locus_tag="ACHJ48_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO4708202.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTI
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITQDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGNYLSIPQNF
                     RVNNIQLDNTHLAYKLRGLQISAGNAVSFVALTNIEMKRASLELHNKPQHLFLRNIKV
                     MQESSVGPALIMNFDMRKDVRGVFMAKEETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23335..24330
                     /locus_tag="ACHJ48_002669"
     CDS             23335..24330
                     /locus_tag="ACHJ48_002669"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO4708203.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24573..25466
                     /gene="galF"
                     /locus_tag="ACHJ48_002670"
     CDS             24573..25466
                     /gene="galF"
                     /locus_tag="ACHJ48_002670"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO4708204.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6295 a         6338 c         6895 g         5985 t
ORIGIN      
        1 taaaaaccat attgaatgac acttaatata attcttaaaa atagccaatt gccgaattgt
       61 taccttgcct gctattccgt tagctgtaac acttcctcct gcattattgg agagccaata
      121 ttcaattacc ataatgtcac cttatattcg gaagttaatt tccgcaataa ccctttctca
      181 tcaacgattg tatgcagaga gggtttgaaa aattaagcta aatcagtgta ttggtagcta
      241 aaaagccagg ggcggtagcg tgtctggatg cctgaaagac cagtctgaat tattctgtca
      301 atagcctgcg gatgaaatca atttttttag gactgatgcc agttaaattt ttattcaatt
      361 aactgcaagg taaatgtgca tggcgacatt gctattaata gtgcacagga taattactct
      421 gccaaagtga taaataaaca atgatgaaat ccaaaatgaa attgatgcca ttattggtgt
      481 cagtaacctt gataagcggt tgcacagtac ttccgggcag caatatgtcg acgatgggca
      541 aagacgtcat caaacagcag gacgctgatt tcgatctcga caaaatggtg aatgtttatc
      601 cgctgacccc gcgcctgatt gaccaattac gcccacgccc gaatgtagcg cgccccaata
      661 tgacgctgga aagtgagatc gcgaattacc agtatcgcgt cgggccgggg gacgttctta
      721 atgtcaccgt ctgggatcac ccggaactca ccacgccagc cggtcagtac cgcagctcca
      781 gcgacaccgg caactgggta cagcctgacg gcactatgtt ttacccgtat atcggcaagg
      841 ttcacgtagt cgggaaaacg ctcgctgaaa tccgcagtga tattaccggg cgcttagcga
      901 cgtacatcgc tgacccgcag gtggacgtta atatcgccgc cttccgctca caaaaggcct
      961 atatctcagg tcaggtgaat aaatctggtc aacaggcgat caccaacgta ccactgacca
     1021 ttctcgacgc catcaacgcc gcaggtggcc tgaccgacac cgctgactgg cgcaacgtgg
     1081 tgctaacaca caatggtcgt gaagagcgaa tttctttgca ggcgctgatg caaaacggcg
     1141 acctcaatca gaaccgactg ctttaccccg gcgatattct ctacgtgccg cgtaatgatg
     1201 atctgaaagt gtttgtgatg ggtgaagtga agaaacagag caccctgaaa atggacttta
     1261 gcggcatgac cctgaccgaa gccctgggca atgctgaagg catcgacatg accacctcca
     1321 acgccagcgg catctttgtc attcgtccgc tgaaaggcga gggcgggcgt aacggcaaaa
     1381 ttgccaatat ctaccagctg gatatgtccg atgccacgtc gctggtgatg gcgacagaat
     1441 tccgcctgca accttatgac gtggtgtatg tcaccaccgc cccggtttcc cgctggaacc
     1501 gtctgatcaa tcagttgctg ccaactatta gcggtgtccg ttacatgacg gatacagcca
     1561 gcgacattca taactggtaa tcgtcatgtt taacaacatc ttagttgtct gtgtcggcaa
     1621 tatttgccgt tccccgacgg cggaacgctt actgcaacgt tatcacccgg agctgaaagt
     1681 ggagtccgct ggactcggcg cgctggtcgg taagggcgct gatcctaccg ctatcagcgt
     1741 cgccgcagaa catcaactgt ctctggaagg ccactgtgca cgacaaatca gccgccgcct
     1801 gtgtcgcaac tatgacctga ttttgaccat ggaaaagcgc catatcgaac gcttatgcga
     1861 gatggcaccg gagatgcgcg gcaaagtgat gctgtttggt cactgggata acgaatgtga
     1921 aatccccgat ccgtatcgca aaagccggga aacgtttgca gcggtgtaca cattacttga
     1981 acggtctgcc cgccagtggg cgcaggcatt gaacgcagag caggtataag aatgacagaa
     2041 aaagtaaaac aacatgccgc tccggtaacg ggcagtgatg aaatcgatat tggtcgcctg
     2101 gtcggcaccg tcattgaagc gcgctggtgg gtgattggca tcaccgctgt attcgccctt
     2161 tgtgccgtgg tttacacctt cttcgccacg ccgatttata gtgccgacgc actggtacaa
     2221 atcgagcaaa gcagcggcaa ttcgttagtg caggatatcg gatcggcgtt agccaacaaa
     2281 ccgcctgcat cggacgccga gatccagttg attcgttcgc gcctggtgct tggtaaaacg
     2341 gtggatgatc tcgacctcga tattgcggtg agcaaaaaca cgttcccgat tttcggtgcg
     2401 ggctgggatc gcctgatggg acgccagaac gagacggtga aagtgactac ctttaaccgt
     2461 ccgaaagaga tggaggatca ggtgtttacg cttaatgtgc tggacaacaa aaactacacc
     2521 ctgagcagcg atggcggctt tagcgcccgt gggcaagcgg gccagatact gaaaaaagaa
     2581 ggcgtcacgc tgatggttga agccattcac gcccgcccgg gcagtgagtt taccgtcacc
     2641 aaatactcca cgctggggat gatcaatcaa ctgcaaaaca gcctgacggt aacggagaac
     2701 ggcaaagacg caggcgtact gagcctgact tataccggtg aagatcgcga acagatccgc
     2761 gacattctta acagcatcgc ccgtaactat caggaacaaa atattgagcg caaatcggcg
     2821 gaagcgtcga aaagcctcgc tttcctcgcg caacagttac cggaagtacg tagccgcctt
     2881 gatgttgccg aaaacaaact gaatgccttc cgtcaggata aagattctgt tgatctgccg
     2941 ctggaagcga aagcggtgct cgattcgatg gtgaacatcg acgcccagtt gaacgaactg
     3001 acctttaaag aggcggaaat ctccaagctg tacaccaaag ttcaccccgc gtaccgcacg
     3061 ctgctggaga aacgtcaggc gctggaagac gaaaaagcca aacttaatgg tcgcgtaacg
     3121 gcgatgccga aaacccagca ggaaattgtc cgtctgaccc gcgatgtcga gtctggtcag
     3181 caggtctata tgcaactgct gaataaagag caggagctga aaatcaccga ggccagcacc
     3241 gtcggcgatg tgcgcattgt tgacccggca atcactcagc ctggtgtgct aaaaccgaag
     3301 aaagggctga ttatccttgg ggcgattatc cttggcctga tgctctctat cgtgggggtg
     3361 ctgctgcgct cgttgtttaa tcgcggcatc gaaagcccgc aggtgctgga agaacacggt
     3421 atcagcgtct atgccagcat cccgctgtcg gagtggcaga aagcgcgcga tagcgtcaaa
     3481 accatcaaag ggattaaacg ctataaacag agccagctac tggcggtggg gaatccaacc
     3541 gatctggcga ttgaagccat ccgcagcctt cgtaccagtt tgcacttcgc gatgatgcag
     3601 gcgcagaaca atgtgttgat gatgaccggg gttagcccgt caatcggtaa aacctttgtc
     3661 tgcgccaacc tggcggcggt gatcagccag accaataaac gcgtgttgtt gatcgactgc
     3721 gatatgcgca aaggctacac ccatgagctg ttgggcacta ataacgttaa tggcctgtcg
     3781 gaaattctga ttggtcaggg cgatattact acagctgcta aaccgacctc tattgccaaa
     3841 tttgacctga tcccgcgcgg tcaggtaccg ccaaatcctt ctgaactgtt gatgagcgaa
     3901 cgctttgccg aactggtgaa ctgggcgagt aaaaactacg acctggtgtt gattgatacg
     3961 ccgccgattc tggcagtgac cgatgcggca attgttggtc gtcatgtcgg aaccacgtta
     4021 atggtggcgc gttatgcggt caacacattg aaagaagtgg aaaccagtct gagccgcttt
     4081 gagcaaaacg gtattccggt gaaaggggtg attctgaact ccatcttccg ccgcgccagc
     4141 gcgtatcagg attatggcta ttacgaatac gaatataagt cggatgcgaa ataacgaggc
     4201 ctgcattcgc accgccccgt aggccggata aggcgctcac gccgcatccg gcaagcaaac
     4261 cagctcataa gccgggagta caacctatga aagacaatcc gctgatctca atctatatgc
     4321 cgacctggaa ccgccaacaa ctggcgattc gggcgataaa atcggtcttg cgccaggact
     4381 acagcaactg ggagatgatc atcgtggatg attgctccac ctcctgggaa caactgcaac
     4441 agtacgtcac cgccctcaac gatccgcgta ttacctacat tcacaacgac attaacagcg
     4501 gggcgtgcgc ggtacgtaac caggcgatta tgctggcgca aggggaatac attaccggga
     4561 tcgatgacga tgatgaatgg acgcccaacc gcctgagcgt cttcctcgcc cataaacagc
     4621 aactggtcac tcacgccttt ttgtacgcta acgattacgt ttgccagggc gaagtttatt
     4681 cccaaccggc aagcttacca ctgtatccca aatcgccgta ctcacgccgc ctgttctata
     4741 aacgcaatat cattggcaat caggtcttta cctgggcatg gcgtttcaaa gagtgtttgt
     4801 tcgataccga actgaaagcg gcacaggatt acgacatctt cctgcggatg gtggtggagt
     4861 atggcgaacc gtggaaagtg gaagaggcga cgcagatcct gcatatcaat cacggtgaaa
     4921 tgcagatcac ctcgtcaccg aaaaaattct ccggatactt ccacttttac cgcaagcaca
     4981 aagacaaatt cgaccgtgcc agtaaaaaat atcagctgtt taccctctac cagatccgca
     5041 ataaacgtat gacctggcgc actttgctga cgctgctgtc ggtgcgtaac ggcaaacgtc
     5101 tggctgatgg tattcggggg cgctaaatat gctggaagat ctacgcgcca acagctggag
     5161 tttacgcccg tgctgcatgg ttcttgccta tcgcgtcgct catttttgtt cggtgtggcg
     5221 caaaaagaac gttctcaaca atctttgggc ggccccgctg ctggtgctgt atcgcattat
     5281 caccgaatgc tttttcggtt atgaaatcca ggctgccgcg accattggcc gccgctttac
     5341 tatccatcac ggttacgccg tagtgatcaa taaaaacgtg gtagcggggg atgatttcac
     5401 cattcgccac ggcgtcacta tcggcaatcg tggtgccgat aacatggcat gtccacacat
     5461 tggcaacggc gtcgaactcg gtgccaacgt cattattctt ggtgatatca cgcttggtaa
     5521 caacgtcacc gtgggcgcgg gcagcgtggt gctcgactct gtcccggaca acgcgctggt
     5581 ggtgggagaa aaagcgcgag tgaaggtaat taaatgaata ttctgcaatt taatgtgcga
     5641 ctggcggaag gcggggcggc aggtgtggcg ttagatctcc accagcgtgc gctgcaacag
     5701 gggctggcgt cacattttgt ctacggctac ggcaaaggcg gcaaagagag cgtcagccat
     5761 cagaactatc cgcaggtcat caaacatacg ccgcggatga ccgcaatggc gaatattgcc
     5821 ctgtttcgtc tgtttaatcg cgatctgttt ggcaatttca atgagttata tcgcaccatt
     5881 actcgtacac cgggtccggt ggtcctgcat tttcatgtgc tgcacagcta ctggctaaat
     5941 cttaagagcg tggtgcgctt ttgcgaaaag gtgaaaaacc acaaaccgga tgtcactctg
     6001 gtctggacgc tgcacgacca ctggagcgtt accgggcgct gcgcctttac cgacggttgt
     6061 gaaggctgga aaacgggctg ccagaaatgc ccgaccttaa ataactatcc gccggtgaag
     6121 attgatcggg cgcaccagct ggtggcgggc aaacgccagt tattccgtga gatgctggcg
     6181 ctgggctgtc agtttatttc ccccagccag catgtggctg acgctttcaa tagcctgtac
     6241 ggtccagggc gttgccggat tatcaataac ggtattgata tggcaaccga ggcgattctg
     6301 gcggatttgc ctccggtgcg cgaaactcag ggcaagccga aaatcgcggt ggtagcgcat
     6361 gacctgcgtt acgacggcaa aactaaccag caactggtac gcgagatgat ggcgctgggc
     6421 gacaaaatcg aactgcatac ctttggtaag ttctcgccgt tcaccgctgg caacgtggtt
     6481 aatcacggct ttgaaactga caagcgcaag ttgatgagcg cgctcaatca gatggatgcg
     6541 ctggtgttca gttctcgcgt cgataactac ccgctgattt tgtgtgaggc gttatcaatt
     6601 ggcgtgccgg tgattgccac ccatagcgac gcggcgcgtg aagtgttgca aaaatccggc
     6661 ggtaaaaccg tcagcgaaga agaggtgctg caactggtcc agttaagcaa accggaaatc
     6721 gcgcaggcgt tatttggtac cacgctggct gagttcagcc aacgcagccg cgccgcctac
     6781 agtggacaac agatgctgga ggagtatgtc aacttctatc agaatctgta gctacctgct
     6841 gctgccgctg atctacctgc tggttaacgt caaaatcacc cagcttggcg aaagtttccc
     6901 catcaccatc gtcacttttt tacctgtctt gttactgctg tttttagaac gcatcagcgt
     6961 taaaaaattg atgattgcct tagggatttg cgcgggactc acggcgttta actacctgtt
     7021 tggtcagtcg ctggatgcca gtaaatacgt cacttcaact atgctgtttg tctatatcgt
     7081 gatcattatt ggtatggtgt ggagtattcg ctttaaaacg atttcgccac acaaccatcg
     7141 caagatatta cgtttctttt atctggtggt cgggctggtg gtggcgctgg cggcggtgga
     7201 gatggcgcaa attatcctca ccggcggcag cagtattatg gagtcgattt cgaaatatct
     7261 gatttacagc aacagctatg tgctgaattt catcaaattc ggcggcaagc gcacgacagc
     7321 actttatttc gaaccggcat ttttcgctct ggcattaatc tcaatttggc tcagcatcaa
     7381 acagtttggt atcaaaacgc ctaaaacaga tgctatgatt ctcgcaggga taatattatc
     7441 cggatcgttt tcaggggtta tgacctttat cctgttttat ttgctggagt gggcatttca
     7501 atatctgaat aaagaggcga ttaagaaaaa gttaccgtta gcattgattt ctctggcagt
     7561 attcctggtt ggtgtggtaa ttgcatttcc ttatatttcc acccgtctgg gcgatttagg
     7621 tacggaagga tcgtcatcgt attatcgtat tgtcggtccg ctggtgatgg tcggttattc
     7681 tttgacccat attgacggtg tagtcagatt tggctcactt tatgaatatg tcgcatcatt
     7741 cggaatattt aacggtgcgg atgtcggaaa aaccatagac aatggtttgt atctgctgat
     7801 tatttatttt tcttggttcg cggtgttttt atcgctgtgg tacatgggga aagtgataaa
     7861 aatgatgatc aacgcttttg gtgataaccg caattttcgc gtgcaattat atctttttac
     7921 tccggtatcg ctgtttttta ccggttcgat atttagcccg gaatatgcat ttttaatcgt
     7981 ctgtccgttt attttgcgaa aagcgttaaa tattacgagg taagaataag aacatgttgc
     8041 ttagcataat cactgtcgcg tttcgtaacc tcgaagggat agtcaaaaca catgcctcgc
     8101 tggcgcatct ggcgcaggcg gaagatatca gcttcgaatg gattgttgtc gatggcggtt
     8161 ccaatgacgg cactcgtgag tacctggaaa atctcaatgg tatctataac ctacgctttg
     8221 tcagcgagcc agataacggt atctacgacg ccatgaacaa gggcattgcg atggcacaag
     8281 gcaagttcgc gttgtttctc aactcgggcg atatttttca tcaggatgcc gcatattttg
     8341 tccgtaagtt aaaaatgcaa aaagataacg tgatgatcac cggcgatgcg ctgctggatt
     8401 ttggcgacgg gcataaaatt aaacgtagcg ccaaaccggg ctggtatatt tatcacagcc
     8461 tgcccgccag tcatcaggcg atatttttcc cggtatccgg tttgaaaaaa tggcgttatg
     8521 acctggaata taaagtttct tctgattacg cgctggcagc caaaatgtat aaagcaggtt
     8581 atgcatttaa aaaactcaat ggcctggtgt ctgaattttc tatgggtggg gtatctacca
     8641 ccaataatat ggaattgtgt gctgacgcga aaaaagtcca acggcaaata ttacatgtgc
     8701 ctggcttttg ggctgaatta tcctggcatt tacgccagcg tactacctca aagacgaaag
     8761 ccttatataa caaaagctga atataaggga aaacgatgca ggatttaagc ggtttctcgg
     8821 tgccgaaagg gttccggggc ggcaacgcta ttaaagtgca attgtggtgg gcagtacagg
     8881 caacaatatt tgcctggtcg ccacaagtat tgtatcgctg gcgggcattt ttattacgtt
     8941 tattcggcgc aaaaatagga aaaaacgtag ttattcgtcc gtcagtaaaa attacctatc
     9001 cgtggaaatt aaccttaggt gattacgtgt gggtcggcga tgacgtcaat ttatataccc
     9061 tcggtgaaat aaccattggc gcacattcgg tgatatcgca aaaaagttat ttatgcaccg
     9121 ggagccacga ccatgcaagt caacatttca ccattaacgc cacgcctatt gtgattggcg
     9181 agaaatgctg gctggcaacc gatgtttttg ttgccccagg cgtcacgatt ggcgacggca
     9241 ccgtcgtggg tgcgcgaagc agtgttttta aatcgcttcc ggcaaatgtg gtttgccggg
     9301 ggaatcccgc agtggtgata cgcaaacgcg ttgaaactga ataaattcaa aaaaaataca
     9361 gaggaataag acatgtcaaa agtcgctctc atcaccggtg taaccggaca agacggttct
     9421 tacctggcag agtttctgct ggaaaaaggt tacgaggtgc atggtattaa gcgtcgcgca
     9481 tcgtcattca acaccgagcg cgtggatcac atttatcagg atccgcacac ctgcaacccg
     9541 aaattccatc tgcattatgg cgacctgagt gatacctcca acctgacgcg tattttgcgt
     9601 gaagtgcagc cggatgaagt gtacaacctg ggcgcaatga gccacgttgc ggtttctttt
     9661 gagtcaccgg aatataccgc tgacgtcgac gcgatgggta cgctgcgcct gctggaggcg
     9721 atccgcttcc tcggtctgga aaagaaaacc cgtttctatc aggcttccac ctctgaactg
     9781 tatggtctgg tgcaggaaat tccgcagaaa gagaccacgc cgttctatcc gcgctctccg
     9841 tatgcggtcg ccaaactgta tgcctactgg atcaccgtca actaccgtga atcctacggc
     9901 atgtatgcct gtaacggtat tctcttcaac catgaatccc cgcgccgcgg cgaaaccttc
     9961 gttacccgca aaatcacccg cgcaatcgcc aacatcgccc aggggctgga gtcgtgcctg
    10021 tacctcggca atatggattc cctgcgtgac tggggccatg ccaaagacta cgtaaaaatg
    10081 cagtggatga tgctgcaaca ggaacagcca gaagatttcg ttatcgcgac cggcgttcag
    10141 tactccgtac gtcagttcgt agaaatggcg gcagcacagc tgggcatcaa actgcgcttt
    10201 gaaggcacgg gcgttgaaga gaagggcatt gtggtttccg tcaccgggca tgacgcgccg
    10261 ggtgttaaac cgggtgatgt gattatcgcc gttgatccgc gttacttccg tccggctgaa
    10321 gttgaaacgc tgctcggcga cccgaccaaa gcgcacgaaa aactgggctg gaaaccggaa
    10381 atcaccctca gagagatggt gtctgaaatg gtggctaatg acctcgaagc ggcgaaaaaa
    10441 cactctctgc tgaaatctca cggctacgac gtggcgatcg cgctggagtc ataagtatga
    10501 gtagacaacg catttttatt gctggccatc gcgggatggt cggttccgcc atcaggcggc
    10561 agctcgaaca gcgcggtgat gtggaactgg tattacgcac ccgcgacgag ctgaacctgc
    10621 tggacagccg cgccgtgcat gatttctttg ccagcgaacg tattgaccag gtctatctgg
    10681 cggccgcgaa agtgggcggc attgttgcca acaacacgta tccggcggat ttcatctatc
    10741 agaacatgat gattgagagc aacatcattc acgccgcgca tcagaacgac gtgaacaaac
    10801 tgctgtttct cggatcgtcc tgcatctacc cgaaactggc aaaacagccg atggcagaaa
    10861 gcgagttatt gcagggcacg ctggagccga ccaacgagcc ttacgccatt gccaaaatcg
    10921 ccgggatcaa actgtgcgaa tcatacaacc gccagtacgg acgcgattac cgctcagtca
    10981 tgccgaccaa cctgtatggc ccgcatgaca acttccaccc gagtaattcg catgtgatcc
    11041 ctgcattgct gcgtcgcttc cacgaggcga cggcacagaa tgcaccggac gtggtggtgt
    11101 ggggcagcgg tacgccgatg cgagaatttc tgcacgtcga tgatatggcg gcggcgagca
    11161 ttcatgtcat ggagctggcg catgaagtct ggctggagaa cacccagccg atgctgtcgc
    11221 acattaacgt cggcacgggc gttgactgca ctatccgcga gctggcgcaa accatcgcca
    11281 aagtggtggg ttacaaaggt cgggtggttt ttgatgccag caaaccggat ggtacgccgc
    11341 gcaaactgct ggatgtgacg cgcctgcatc agcttggctg gtatcacgaa atctcactgg
    11401 aagcggggct tgccagcact taccagtggt tccttgagaa tcaagaccgc tttcgggggt
    11461 aatgatgttt ttacgtcagg aagactttgc cacggtagtg cgctccacac cgcttgtctc
    11521 tctcgacttt attgtcgaga acagtcgcgg cgagtttctg cttggcaaaa gaaccaaccg
    11581 cccggcgcag ggttactggt ttgtgccggg agggcgcgtg cagaaagacg aaacgctgga
    11641 agccgcattt gagcggctga cgatggcgga actggggctg cgtctgccga taacagcagg
    11701 ccagttttac ggtgtctggc agcactttta tgacgataac ttctccggca cggatttcac
    11761 cactcactat gtggtgctcg gttttcgctt cagagtagcg gaagaagaac tgttactgcc
    11821 ggatgagcag catgacgatt accgctggct gacgccggac gcgctgctcg ccagtaacga
    11881 tgttcacgct aacagccgcg cctattttct cgctgagaag cgtgccggag tacccggatt
    11941 atgaaaatac tggtctacgg cattaactac tcgccggagt taaccggcat cggcaaatac
    12001 accggcgaga tggtggaatg gctggcggcg caaggtcatg aggtgcgggt tattaccgca
    12061 ccgccttact acccgcagtg gcaagtgggc gagaactatt ccgcctggcg gtacaaacga
    12121 gaagaggggg ccgctacggt gtggcgctgc ccgctgtacg tgccaaaaca gccgagcacc
    12181 ctgaaacgcc tgttgcatct cggcagtttt gccgtcagca gttttttccc gttgatggcg
    12241 caacgtcgct ggaagccgga tcgcattatc ggcgtagtgc caacgctgtt ttgcacgccg
    12301 ggaatgcgcc tgctggcgaa actctctggc gcgcgtaccg tgctgcatat tcaggattac
    12361 gaagtagatg ccatgctggg gctgggcctt gccggaaaag gcaaaggcgg caaagtggca
    12421 cagctggcaa cggcgttcga acgtagcgga ctgcataacg tcgataacgt ttccacgatt
    12481 tcgcgttcga tgatgaataa agccatcgaa aaaggcgtgg cggcggaaaa cgtcatcttc
    12541 ttccccaact ggtcggaaat cgcccgtttt cagcatgttg cagacgccga tgttgatgcc
    12601 cttcgtaacc agcttggcct gccggataac aaaaaaatca ttctttactc cggcaatatt
    12661 ggtgaaaagc aggggctgga aaacgttatt gaagcagccg atcgcctgcg cgatgaaccg
    12721 ctgatttttg ccattgtcgg gcagggcggc ggcaaagcgc ggctggaaaa aatggcgcag
    12781 cagcgtggac tgcgcaacat gcaatttttc ccgctgcaat cgtatgacgc tttacccgca
    12841 ctgctgaaga tgggcgattg ccatctggtg gtgcaaaaac gcggcgcggc agatgccgta
    12901 ttgccgtcga aactgaccaa tattctggca gtaggcggta acgcggtgat tactgctgaa
    12961 gcccacacag aactgggaca gctttgcgaa acctttccgg gcattgcggt ttgcgtagaa
    13021 ccggaatcgg ttgaggcgct ggtggcgggg attcgtcagg cgctcctgct gcccaaacac
    13081 aacacggtgg cacgtgaata tgccgaacgc acgctcgata aagagaacgt gttacgtcaa
    13141 tttataaatg atattcgggg ataattatgg cgcagtcgaa actctatcca gttgtgatgg
    13201 caggtggctc cggtagccgc ttatggccgc tttcccgcgt actttatccc aagcagtttt
    13261 tatgcctgaa aggcgatctc accatgctgc aaaccaccat ctgccgcctg aacggcgtgg
    13321 agtgcgaaag ccctgtggtg atttgcaatg agcagcaccg ctttattgtt gcggaacaac
    13381 tgcgtcaact gaacaaactc actgagaaca ttattctcga accggcaggg cgtaacacag
    13441 caccggccat tgcgctggcg gcgctggcgg caaaacgtca tagcccggag aacgacccat
    13501 taatgctggt gctggcggcg gatcatgtga ttgccgatga agacgcattc cgcgccgccg
    13561 tgcgtaacgc tatgccgtat gccgaagcgg gcaagctggt gaccttcggc attgtgccgg
    13621 atctacctga aaccggatat ggctatattc gtcgcggtga agtgtcggcg ggtgagcagg
    13681 atacggtggc ctttgaagtg gcgcagtttg tcgaaaaacc aaatctggaa accgcgcagg
    13741 cctatgtggc aagcggcgaa tattactgga acagcggtat gttcctgttc cgtgccggac
    13801 gctatctcga agaactgaaa aaatatcgcc cggatattct cgacgcctgt gaaaaagcga
    13861 tgagcgccgt cgatccggat ctcgatttta ttcgcgtgga tgaagacgcg tttctggcct
    13921 gcccggaaga gtcggtggat tacgcggtca tggaacgtac ggcagatgcc gttgtggtgc
    13981 cgatggatgc gggctggagc gatgtcggct cctggtcctc attatgggag atcagcgccc
    14041 acaccgccga gggcaacgtt tgccacggcg atgtgattaa tcacaaaact gaaaacagct
    14101 atgtgtatgc cgaatctggc ctggtcacca ccgtcggggt gaaagatttg gtggtagtgc
    14161 agaccaaaga tgcggtgctg attgccgacc gtaacgcggt gcaggatgtg aaaaaagtgg
    14221 tcgagcagat caaagccgat ggtcgccatg agcatcgggt gcatcgcgaa gtctatcgcc
    14281 cgtggggcaa atatgactct atcgacgcgg gcgaccgcta ccaggtgaaa cgcatcaccg
    14341 tgaaaccggg cgaaggcttg tcggtacaga tgcaccatca ccgcgcggaa cactgggtgg
    14401 ttgtcgcggg aacggcaaaa gtcaccattg acggtgatat caaactgctt ggtgaaaacg
    14461 agtcgattta tattccgctg ggggcgacgc actgcctgga aaacccgggg aaaattccgc
    14521 tcgatttaat tgaagtgcgc tccggctctt atctcgaaga ggatgatgtg gtgcgcttcg
    14581 cggatcgcta cggacgggtg taaacgtcgc atcaggcgct gattgtcgga tgcggcgtga
    14641 acgccttatc cgacctacgg ttcggttttg taggcctgat aagacgcggc agcgtcgcat
    14701 caggcaatga atgcgaaacc gcggtgtaaa taacgacaaa aacaaaattg gccgtttcgg
    14761 tcagggccaa ctattgcctg aaaaagggta acgatatgaa aaaattaacc tgctttaaag
    14821 cctatgatat tcgcgggaaa ttaggcgaag aactgaatga agatattgcc tggcgcattg
    14881 gtcgtgccta tggcgaattt ctcaaaccga aaaccattgt gttaggcggt gatgcccgtc
    14941 tcaccagcga aaccttaaaa ctggcgctgg caaaaggttt acaggatgcg ggcgttgacg
    15001 tgctggatat tggtatgtcc ggcaccgaag agatctattt cgccacgttc catctcggcg
    15061 tggatggcgg cattgaagtt actgccagcc ataacccgat ggattacaac ggcatgaaac
    15121 tggtgcgcga aggggctcgc ccgatcagcg gcgataccgg actgcgcgat gtccagcgtc
    15181 tggcagaagc caacgacttt cctcccgttg atgaaaccaa acgcggtcgc tatcagcaaa
    15241 tcaatctgcg tgacgcttac gttgatcacc tgttcggtta tatcaacgtc aaaaacctta
    15301 cgccgctcaa gctggtgatc aactccggga acggcgcggc gggtccggtg gtggacgcca
    15361 tcgaagcccg ctttaaagcc ctcggcgcac cggtggaatt gatcaaagtg cacaacacgc
    15421 ccgacggcaa tttccccaac ggtattccta acccgctgct gccggaatgt cgcgacgaca
    15481 cccgcaatgc ggtcattaaa cacggcgcgg atatgggcat tgcttttgat ggcgattttg
    15541 accgctgttt cctgtttgac gaaaaagggc agtttatcga gggctactac attgtcggtc
    15601 tgctggcaga agcgttcctc gaaaaaaatc ccggcgcgaa gatcatccac gatccacgtc
    15661 tctcctggaa taccgttgat gtggtgactg ccgcaggcgg caccccggta atgtcgaaaa
    15721 ccggacacgc ctttattaaa gaacggatgc gcaaggaaga cgccatctac ggtggcgaaa
    15781 tgagcgccca ccattacttc cgtgattttg cttactgcga cagcggcatg atcccgtggc
    15841 tgctggtcgc cgaactggtg tgcctgaaag gaaagacgct gggcgaactg gtgcgcgacc
    15901 ggatggcagc gtttccgtca agcggtgaga tcaacagcaa actggcgcaa cccgttgagg
    15961 cgattaaccg cgtggaacag cattttagcc gtgaggcgct ggcggtggat cgcaccgatg
    16021 gcatcagcat gacctttgcc gactggcgct ttaatctgcg ctcctccaac accgaaccgg
    16081 tggtgcggtt gaatgtggaa tcacgcggtg atgtgccgct gatggaagcg cgaacgcgaa
    16141 ctctgctgac gttgctgaac gagtaatgtc ggatcttccc ttaccccatt gcgggtaagg
    16201 ggctaataac aggaacaacg atgacaaatc taaaaaagcg cgagcgagcg aaaaccaatg
    16261 catcgttaat ctctatggtg caacgctttt cagatatcac catcatgttt gccggactat
    16321 ggctggtttg cgaagtgagc ggactgtcat tcctctacat gcacctgttg gtggcgctga
    16381 ttacgctggt ggtgttccag atgctgggcg gcatcaccga tttttatcgc tcatggcgcg
    16441 gtgttcgggc agcgacagaa tttgccctgt tgctacaaaa ctggacctta agcgtgattt
    16501 tcagcgccgg actggtggcg ttcaacaatg atttcgacac gcaactgaaa atctggctgg
    16561 cgtggtatgg gctgaccagt atcggactgg tggtttgccg ttcatgtatt cgcattgggg
    16621 cgggctggct gcgtaatcat ggctataaca agcgcatggt cgcggtggcg ggggatttag
    16681 ccgccgggca aatgctgatg gagagcttcc gtaaccagcc gtggttaggg tttgaagtgg
    16741 tgggcgttta ccacgacccg aaactgggcg gcgtttctaa cgactgggcg ggtaacctgc
    16801 aacagctggt cgaggatgct aaagcaggca agattcataa cgtctatatc gcgatgcaaa
    16861 tgtgcgacgg cgcgcgagtg aaaaaactgg tccatcaact ggcggacacc acctgttcgg
    16921 tgctgctgat ccccgatgtc tttaccttca acattctcca ttcacgtctt gaagagatga
    16981 acggcgttcc ggtggtgccg ctttacgaca cgccgctttc cggggttaac cgcctgctca
    17041 aacgtgcgga agacattgtg ctggcgacgc ttatcctgct gctgatctcc ccggtgctgt
    17101 gctgcattgc gctggcggtg aaactcagtt cacctgggcc ggttattttc cgccagactc
    17161 gctacggcat ggatggcaag ccgatcaaag tgtggaagtt ccgttccatg aaagtgatgg
    17221 agaacgacaa agtggtgacc caggcgacgc agaacgatcc gcgcgtcacc aaagtgggga
    17281 actttctgcg ccgcacctcg ctggatgaat tgccgcagtt tatcaatgtg ctgaccgggg
    17341 ggatgtcgat tgtcggtcca cgtccgcacg cggtggcgca taacgaacag tatcgacagc
    17401 tcattgaagg ctacatgctg cgtcataagg tgaaaccggg cattaccggc tgggcgcaga
    17461 ttaacggctg gcgcggcgaa accgacacgc tggagaaaat ggaaaaacgc gtcgagttcg
    17521 accttgagta catccgcgaa tggagcgtct ggttcgatat caaaatcgtt ttcctgacgg
    17581 tattcaaagg attcgttaac aaagcggcat attgatatga gcttacgtga aaaaaccatc
    17641 agcggcgcga agtggtcggc gattgccacg gtgatcatca tcggcctcgg gctggtgcag
    17701 atgaccgtgc tggcgcggat tatcgacaac caccagttcg gtttgcttac cgtgtcgctg
    17761 gtgatcatcg cgctggcaga tacgctttct gacttcggta ttgctaactc gattattcag
    17821 cgaaaagaaa tcagccacct tgaactcacc acgctgtact ggctgaacgt tgggctgggg
    17881 cttgtggtgt gtgtggcggt gtttttgttg agtgatctca ttggtgacgt gctgaacaac
    17941 ccggacctgg caccgttgat taaaacattg tcgctggcgt ttgtggttat cccccacggg
    18001 caacagttcc gcgcgttgat gcaaaaggag ctggagttca acaaaatcgg catgatcgaa
    18061 accagcgcgg tactggcggg cttcactttt acggtggtta gcgcccattt ctggccgctg
    18121 gcgatgaccg cgatcctcgg ttatctggtc aatagtgcgg tgagaacgct gctgtttggc
    18181 tactttggcc gcaaaattta tcgccccggt ctgcatttct cgctggcgtc tgttgcgccg
    18241 aacttacgct ttggtgcctg gctgacggcg gacagcatca tcaactatct caataccaac
    18301 ctttcaacgc tggtgctggc gcgtattctc ggcgcgggcg tggcaggggg atacaacctg
    18361 gcgtacaacg tggccgttgt gccgccgatg aagctgaacc cgatcatcac ccgtgtgttg
    18421 tttccggcat tcgccaaaat tcaggacgat accgaaaagc tgcgcgtcaa cttctacaag
    18481 ctgctgtcgg tggtggggat tatcaacttt ccggcgctgc tcgggctgat ggtggtgtca
    18541 aataactttg taccgctggt atttggcgaa aagtggaaca gcattattcc ggtgctgcaa
    18601 ttgctgtgtg tggtgggtct gctgcgctcc gtaggtaacc cgattggttc gctgctgatg
    18661 gcgaaagcgc gggtcgatat cagctttaaa ttcaacgtat tcaaaacatt tctgtttatt
    18721 ccggcgattg ttattggtgg gcagatggcg ggcgcaatcg gcgtcacgct cggtttcctg
    18781 ctggtgcaga ttatcaacac cattctgagt tacttcgtga tgattaaacc ggttcttggt
    18841 tccagttatc gccagtacat cctgagtttg tggctgccgt tttatctctc gctgccgacg
    18901 ctggtggtca gttatgtgct gggcattgtg ctgaaagggc aactggcgct ggggatgctg
    18961 ctggcggtgc aaatagccgc gggcgtgctg gcgtttgtgg tgatgattgt gctgtcgcgc
    19021 catccgctgg tggtggaagt gaagcgccag ttttgtcgca gcgaaaaaat gaaaatgctt
    19081 ttacgggcgg ggtgagttgt tgttttcggt tccgacaacg gttgccgggt gcagcgtaaa
    19141 acaccttatc catcctacgg ttctgtgctc gggtcttgta ggcctgataa gacgcgccag
    19201 cgtcgcatca ggcaaaaaac ggcaacgaac acaacaaaca cagcctcacc tggcatcaaa
    19261 tttatgagga aaaaatgaaa ttacttattc tgggcaacca cacttgcggc aatcgtggcg
    19321 acagcgccat cttgcgcggc ttacttgatg ccatcaacat cctcaatcca cataccgaag
    19381 tggacgtgat gagccgctat ccggtcagtt cttcctggct gctcaaccgc ccggtaatgg
    19441 gtgatccgct gttccttcaa atgaaacaac acaacagcgc ggcgggcgtt gtcgggcgcg
    19501 ttaaaaaagt cctccgtcgc cgctatcagc accaggtatt gctctcacgc gtcaccgaca
    19561 ctggcaagct gcgcaatatc gccatcgccc agggattcac cgacttcgtg cgcctactgt
    19621 caggttacga cgccattatt caggtcggcg gatcgttttt tgtcgatctc tacggcgtgc
    19681 cgcagtttga acatgcactt tgcacattta tggcgaaaaa gccgctgttt atgattggtc
    19741 acagcgtcgg tcccttccag gatgagcaat ttaaccaact ggcgaactac gtttttggtc
    19801 actgcgacgc gctgatcttg cgcgaatcgg tcagcctcga tctaatgaaa cgcagcaata
    19861 tcaccactgc aaaagttgaa catggcgtcg ataccgcgtg gctggtcgat caccacacag
    19921 aagacttcac cgccagctat gccgtccaac actggctgga cgttgccgca caacagaaaa
    19981 cggtggcaat taccctgcgc gaactggcac cgttcgacaa acgcctcggc accactcagc
    20041 aagtgtatga aaaagccttt gccggggtgg tcaatcgcat tctcgacgaa ggctatcagg
    20101 tcattgcgct ctccacctgt acgggtatcg acagctataa caaagacgac cgcatggtgg
    20161 cgctcaacct gcgccagcac atcagcgatc ctgcccgtta tcacgtagtg atggatgaac
    20221 ttaacgatct ggaaatgggc aaaattctcg gtgcctgtga actcaccgtc ggtacgcgcc
    20281 tgcactccgc cattatctca atgaactttg ctactccggc gattgccatc aactatgaac
    20341 ataaatccgc cggggttatg cagcagctgg gactaccgga gatggcaatt gatatccgtc
    20401 atttattaga cggcagcctg caagcgatgg ttgcggatac cttaggccag cttccggcgc
    20461 tgaacgcacg acttagcgaa gccgtcagtc gtgagcgtca gacgggaatg cagatggtgc
    20521 agtccgtact ggaacgcatc ggggaggtga aatgaaggtc ggcttcttct tattgaaatt
    20581 tccgctgtcg tcagaaacct tcgttcttaa ccaaatcact gcgtttattg atatgggctt
    20641 tgaagtggag attgtcgcgc tgcaaaaagg cgacacacaa aacacccacg tggcatggac
    20701 taaatacaac cttgccgcca gaacccgctg gttacaggac gaacctacgg gcaaagtggc
    20761 gaaactgcgc caccgagcca gtcagacctt gcgcggcatt catcgtaaaa atacctggca
    20821 ggcgctcaac ctcaaacgct atggtgccga gtcgtggaac ctgattttgt ctgccatttg
    20881 cggccaggtc gcaacaccgt ttcgcgccga tgtgttcatc gctcattttg gccctgcggg
    20941 ggtaaccgca gcaaaactac gcgaactggg tgtcattcac ggcaaaattg ccactatttt
    21001 ccacggcatt gatatttcca gtcgggaagt gctcaaccat tacactcccg aatatcaaca
    21061 actgtttcga cgtggcgacc tgatgctacc gataagcgat ctgtgggccg gaaggctgca
    21121 aaaaatgggc tgcccgaggg aaaaaatcgc cgtatcgcgc atgggcgtag atatgacgcg
    21181 ctttagcccg cgtcccgtga aagcgcccgc aacaccgctg gagattattt ccgtcgcacg
    21241 cttaaccgag aaaaaaggtc tgcatgtggc gattgaagcc tgccggcagt tgaaagagca
    21301 gggcgtggca tttcgctatc gcatcctcgg cattggcccg tgggaacgac gcctgcgcac
    21361 gctcatcgaa caatatcaac tggaagatgt ggtggagatg ccgggcttta aaccgagcca
    21421 tgaagtgaaa gcgatgctcg acgacgcgga tgtcttcctg ttgccatcgg ttacaggtgc
    21481 ggatggcgat atggaaggca ttccggtggc gctgatggag gcgatggcgg tcggcattcc
    21541 ggtggtttct actctgcata gcggaatacc ggaactggtg gaggctgaca aatccggctg
    21601 gctggtgcct gagaacgatg ctcgcgcact ggcgcaacga ctggcggcgt ttagccaact
    21661 ggacaccgac gaattggctc cggtcgtcaa acgcgcgcgc gaaaaagttg aacacgattt
    21721 taaccagcag gtgatcaatc gagaactcgc cagcttgctg caggctttat agaggagaac
    21781 gtatgccatt taaaaaactc tcccgacgca ccttcctgac ggcaagctcg gcgcttgcct
    21841 tccttcatac ccctttcgct cgcgcacttc ccgcccgaca aagcgttaat atcaacgact
    21901 acaaccctca cgactggatc gcctcattta aacaagcctt cagcgaaggg caaacggttg
    21961 tcgtgcctgc tggattcgtt tgtgacaata tcaacaccgg catcttcatt cctcctggca
    22021 aaacgttaca catccttgga agcctgcgcg gcaacggcag agggcgattt gtcttacagg
    22081 acggcagcca ggtgacaggg gaggagggcg gcagtatgca taacatcacc ctggatgtgc
    22141 gtggttctga ctgcaccatc aaagggctgg cgatgagcgg ctttggtccg gtaacgcaga
    22201 tttatatcgg cggcaaaaac aaacgggtca tgcgcaacct gaccatcgat aacctgacca
    22261 ttagccacgc taattacgcc atcttacgcc agggatttca taaccagatt atcggtgcca
    22321 acatcaccaa ttgtaagttc agcgacttac agggcgatgc cattgaatgg aacgtggcga
    22381 ttaacgaccg cgatattttg atctccgacc atgtcatcga gcgcatcaac tgtactaacg
    22441 gcaaaatcaa ctggggcatt ggcataggcc ttgcgggaag cacttacgat aataattacc
    22501 cggaagatca ggcagtgaaa aactttgtcg tggcgaatat cacgggatcg gattgtcggc
    22561 agttgataca tgttgaaaat ggtaaacatt ttgttattcg taatatcaaa gcccgcaata
    22621 tcacgcagga tttcagtaag aaagcaggta ttgataacgc cacagtcgct atttacggtt
    22681 gtgacaattt cgtgattgat aatattgaaa tgattaatag cgccgggatg ttaatcggct
    22741 atggggtaat taaaggcaat tatctctcga ttccgcaaaa tttccgagtg aataatattc
    22801 aactggataa cacccatctt gcttataaat tgcgcggcct ccaaatctct gccgggaatg
    22861 ccgtctcctt tgtggcacta actaacattg agatgaagcg tgcgtcgctg gagttacaca
    22921 acaaaccgca acatcttttt ctgcgtaata tcaaagtgat gcaggaatcc tctgttggac
    22981 ccgcattgat tatgaacttc gacatgcgca aagacgttcg aggcgtcttt atggcgaaag
    23041 aagaaacact gctgtctctt gcaaatgttc atgcggtgaa tgagaaagga caaagctccg
    23101 tcgatatcga caggattaat caccatattg ttaatgtgga aaagattaac tttagattgc
    23161 cggaacggag agagtagatt tgcgaccatt cataaactta ctggatataa tgttctaaaa
    23221 gattataatc ctcacactaa ctgttaaagc tggggctggg cagcattgac tactcaggga
    23281 aggtactcat ctttatcttg tgtttcttac gtaacaacca gactaaagtg atttatgaac
    23341 gataacgttt tgctcatagg agcttccgga ttcgtaggaa cccgactact tgaaacggca
    23401 attgctgact ttaatatcaa gaacctggac aaacagcaga gccactttta tccagaaatc
    23461 acacagattg gtgatgttcg tgatcaacag gcactcgacc aggcgttagc cggttttgac
    23521 actgttgtac tactggcagc ggaacaccgc gatgacgtca gccctacttc tctctattat
    23581 gatgtcaacg ttcagggtac ccgcaatgtg ctggcggcca tggaaaaaaa tggcgttaaa
    23641 aatatcatct ttaccagttc cgttgctgtt tatggtttga acaaacacaa ccctgacgaa
    23701 aaccatccac acgacccttt caaccactac ggcaaaagca agtggcaggc ggaggaagtg
    23761 ctgcgtgaat ggtataacaa agcaccaaca gaacgttcat taactatcat ccgtcctacc
    23821 gttatcttcg gtgaacgcaa ccgcggtaac gtctataact tgctgaaaca gatcgctggc
    23881 ggcaagttta tgatggtggg cgcagggact aactataagt ccatggctta tgttggaaac
    23941 attgttgagt ttatcaagta caaactgaag aatgttgccg caggttacga ggtttataac
    24001 tacgttgata agccagacct gaacatgaac cagttggttg ctgaagttga acaaagcctg
    24061 aacaaaaaga tcccttctat gcacttgcct tacccactag gaatgctggg tggatattgc
    24121 tttgatatcc tgagcaaaat tacgggcaaa aaatacgctg tcagctctgt gcgcgtgaaa
    24181 aaattctgcg caacaacaca gtttgacgca acgaaagtgc attcttcagg ttttgtggca
    24241 ccgtatacgc tgtcgcaagg tctggatcga actctgcagt atgaattcgt ccatgccaaa
    24301 aaagacgaca taacgtttgt ttctgagtaa tgcagacgag tcagagaaaa aaggaccagg
    24361 ttggactacg attattcgga aagaggtact gattaaatta tcagtatgtg gatttccgag
    24421 taatccgagt aacccagatg ctgatggtgt gtagtgttat cgtaaggttt aatcaaagtt
    24481 atgcggaact aagctttggt atatagccat aacgtttggc taacctatgg caataattta
    24541 cgcactatca gccacttaga ggtaggaaaa gaatgacgaa tttaaaagca gttataccgg
    24601 tagcaggtct tgggatgcat atgttgcctg ccactaaggc gattcccaaa gagatgctac
    24661 cgatcgtcga caagccaatg attcagtaca ttgttgacga gattgtggct gcagggatca
    24721 aagaaatcct cctggtaact cacgcgtcca agaacgcagt cgaaaaccac ttcgacacct
    24781 cttatgaatt agaatctctc cttgaacagc gcgtgaagcg tcaactactg gcggaagtac
    24841 aatctatctg tccgccgggc gtgaccatta tgaacgtgcg tcagggtgaa cctttaggtt
    24901 taggccactc cattttatgt gcgcgacctg ccattggtga caatccattt gtcgtggtgc
    24961 tgccagacgt tgtgatcgac gacgccagcg ccgacccgct acgctacaac cttgctgcca
    25021 tgattgcgcg cttcaatgaa acgggacgca gccaggtgct ggcaaaacgt atgccgggcg
    25081 acctctctga atactccgtc atccagacca aagaaccgct ggatcgtgaa ggcaaagtca
    25141 gccgcattgt tgaatttatc gaaaagccgg atcagccgca gacgctggac tcagacatca
    25201 tggccgttgg tcgatatgtg ctttctgccg atatttggcc ggaactggaa cgtactcagc
    25261 ctggtgcatg gggacgtatt cagctgactg atgccattgc tgagctggca aaaaaacaat
    25321 ccgttgatgc aatgctgatg actggcgaca gttacgactg cggtaaaaaa atgggctata
    25381 tgcaggcgtt tgtgaagtat ggactacgca acttgaaaga aggggcgaag ttccgcaaag
    25441 gtatcgagaa gctgttaagc gaataatgaa aatctgaccg aatgtaacgg ttgataagga
    25501 aataatatcg gca
//
LOCUS       DAZFDC010000063        25365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFDC010000063 DAZFDC010000000
VERSION     DAZFDC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.063"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..737
                     /locus_tag="ACHJ48_002671"
     CDS             54..737
                     /locus_tag="ACHJ48_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708205.1"
                     /translation="MKLPVKLLMSLISLVRVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            753..1163
                     /locus_tag="ACHJ48_002672"
     CDS             753..1163
                     /locus_tag="ACHJ48_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708206.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            1384..2205
                     /locus_tag="ACHJ48_002673"
     CDS             1384..2205
                     /locus_tag="ACHJ48_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4708207.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPQPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2287..2766
                     /locus_tag="ACHJ48_002674"
     CDS             2287..2766
                     /locus_tag="ACHJ48_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4708208.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPEGSAIMRIID"
     gene            2781..3257
                     /gene="yeeS"
                     /locus_tag="ACHJ48_002675"
     CDS             2781..3257
                     /gene="yeeS"
                     /locus_tag="ACHJ48_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO4708209.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3320..3541
                     /gene="yeeT"
                     /locus_tag="ACHJ48_002676"
     CDS             3320..3541
                     /gene="yeeT"
                     /locus_tag="ACHJ48_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4708210.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3615..3983
                     /gene="cbeA"
                     /locus_tag="ACHJ48_002677"
     CDS             3615..3983
                     /gene="cbeA"
                     /locus_tag="ACHJ48_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO4708211.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHPDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            4072..4445
                     /gene="cbtA"
                     /locus_tag="ACHJ48_002678"
                     /pseudo
     CDS             4072..4445
                     /gene="cbtA"
                     /locus_tag="ACHJ48_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4442..4636
                     /gene="yeeW"
                     /locus_tag="ACHJ48_002679"
     CDS             4442..4636
                     /gene="yeeW"
                     /locus_tag="ACHJ48_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4708212.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4682..4762
                     /locus_tag="ACHJ48_002680"
                     /pseudo
     CDS             4682..4762
                     /locus_tag="ACHJ48_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5051..5197)
                     /locus_tag="ACHJ48_002681"
                     /pseudo
     CDS             complement(5051..5197)
                     /locus_tag="ACHJ48_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5251..5433
                     /locus_tag="ACHJ48_002682"
     CDS             5251..5433
                     /locus_tag="ACHJ48_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO4708213.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(5534..5863)
                     /gene="tmaR"
                     /locus_tag="ACHJ48_002683"
     CDS             complement(5534..5863)
                     /gene="tmaR"
                     /locus_tag="ACHJ48_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO4708214.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6035..7093)
                     /gene="yeeA"
                     /locus_tag="ACHJ48_002684"
     CDS             complement(6035..7093)
                     /gene="yeeA"
                     /locus_tag="ACHJ48_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO4708215.1"
                     /translation="MRANKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     GTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(7292..7765)
                     /gene="sbmC"
                     /locus_tag="ACHJ48_002685"
     CDS             complement(7292..7765)
                     /gene="sbmC"
                     /locus_tag="ACHJ48_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO4708216.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(7884..9050)
                     /gene="dacD"
                     /locus_tag="ACHJ48_002686"
     CDS             complement(7884..9050)
                     /gene="dacD"
                     /locus_tag="ACHJ48_002686"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO4708217.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            9259..10686
                     /gene="sbcB"
                     /locus_tag="ACHJ48_002687"
     CDS             9259..10686
                     /gene="sbcB"
                     /locus_tag="ACHJ48_002687"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO4708218.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(10729..10956)
                     /gene="tsuB"
                     /locus_tag="ACHJ48_002688"
     CDS             complement(10729..10956)
                     /gene="tsuB"
                     /locus_tag="ACHJ48_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO4708219.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(10970..12028)
                     /gene="tsuA"
                     /locus_tag="ACHJ48_002689"
     CDS             complement(10970..12028)
                     /gene="tsuA"
                     /locus_tag="ACHJ48_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO4708220.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTVRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(12207..13565)
                     /gene="plaP"
                     /locus_tag="ACHJ48_002690"
     CDS             complement(12207..13565)
                     /gene="plaP"
                     /locus_tag="ACHJ48_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO4708221.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(13555..13617)
                     /gene="yoeI"
                     /locus_tag="ACHJ48_002691"
     CDS             complement(13555..13617)
                     /gene="yoeI"
                     /locus_tag="ACHJ48_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO4708222.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(13832..14761)
                     /gene="yeeY"
                     /locus_tag="ACHJ48_002692"
     CDS             complement(13832..14761)
                     /gene="yeeY"
                     /locus_tag="ACHJ48_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4708223.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHNVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(14807..15631)
                     /gene="yeeZ"
                     /locus_tag="ACHJ48_002693"
     CDS             complement(14807..15631)
                     /gene="yeeZ"
                     /locus_tag="ACHJ48_002693"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4708224.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            16057..16107
                     /gene="hisL"
                     /locus_tag="ACHJ48_002694"
     CDS             16057..16107
                     /gene="hisL"
                     /locus_tag="ACHJ48_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO4708225.1"
                     /translation="MTRVQFKQHHHHHHPD"
     misc_feature    16083..16206
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            16253..17152
                     /gene="hisG"
                     /locus_tag="ACHJ48_002695"
     CDS             16253..17152
                     /gene="hisG"
                     /locus_tag="ACHJ48_002695"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO4708226.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDDAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTQRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            17158..18462
                     /gene="hisD"
                     /locus_tag="ACHJ48_002696"
     CDS             17158..18462
                     /gene="hisD"
                     /locus_tag="ACHJ48_002696"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO4708227.1"
                     /translation="MSFNTIIDWNSCTAKQQRQLLMRPAISASESITRTVNDILDSVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            18459..19529
                     /gene="hisC"
                     /locus_tag="ACHJ48_002697"
     CDS             18459..19529
                     /gene="hisC"
                     /locus_tag="ACHJ48_002697"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO4708228.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            19529..20596
                     /gene="hisB"
                     /locus_tag="ACHJ48_002698"
     CDS             19529..20596
                     /gene="hisB"
                     /locus_tag="ACHJ48_002698"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO4708229.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTRRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            20596..21186
                     /gene="hisH"
                     /locus_tag="ACHJ48_002699"
     CDS             20596..21186
                     /gene="hisH"
                     /locus_tag="ACHJ48_002699"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO4708230.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            21186..21923
                     /gene="hisA"
                     /locus_tag="ACHJ48_002700"
     CDS             21186..21923
                     /gene="hisA"
                     /locus_tag="ACHJ48_002700"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO4708231.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAISCWQNA"
     gene            21905..22681
                     /gene="hisF"
                     /locus_tag="ACHJ48_002701"
     CDS             21905..22681
                     /gene="hisF"
                     /locus_tag="ACHJ48_002701"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO4708232.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            22675..23286
                     /gene="hisIE"
                     /locus_tag="ACHJ48_002702"
     CDS             22675..23286
                     /gene="hisIE"
                     /locus_tag="ACHJ48_002702"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO4708233.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(23383..24360)
                     /gene="wzzB"
                     /locus_tag="ACHJ48_002703"
     CDS             complement(23383..24360)
                     /gene="wzzB"
                     /locus_tag="ACHJ48_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO4708234.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEIVAQEQKELRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(24506..>25365)
                     /locus_tag="ACHJ48_002704"
     CDS             complement(24506..>25365)
                     /locus_tag="ACHJ48_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFO4708235.1"
                     /translation="VVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGK
                     ALYDNLHPSRIVIGERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLA
                     MRVAYFNELDSYAESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLA
                     NYQSVPNNLISAIVDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGI
                     MKRIKAKGVEVIIYEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKV
                     YTRDLFGSD"
BASE COUNT         6542 a         6589 c         6264 g         5970 t
ORIGIN      
        1 ctgagagaaa gttttacccc tcacctgtca attcactcac tggaaattta attatgaaat
       61 tacctgtaaa gttattaatg agccttatat ctctggtccg cgttattgca cgtgccgggg
      121 aatataaaaa ttacagccgg gatgaaataa aatactggcg atatacatca tacaaggggg
      181 ggaaacttcc ggaaggtttc actgatgaga aattttccag cgccatttac aacggaagaa
      241 tatttacaat gaaacgttta cataccctta tgttatttct ggcggttctg ttcacgggct
      301 ttaactttaa cgcagaagcg gctaccgtaa aacaagctct tagttgtaac ccggaagcct
      361 gggctgaaca acctggagcg tgtccatcaa cgtacgagtt gtacgaaggt gacgctacct
      421 acaaagccgc tatcgacaaa gcattaaaac cggtcggact gagcggcatg ttcggtaaag
      481 gcggctatat ggatggccct ggtggaggta tcacgccagt aaacattaac ggtacagtct
      541 ggttccaggg cgacggttgc aaagccaata cctgcggctg ggactttatc gtaacactct
      601 ataacccaaa aacccatgaa gttgttggct accgttactt tggtttagat gacccggcct
      661 acctggtttg gttcggtgaa attggcgtgc atgaattcgc gtatctggtg aaaaactacg
      721 tagctgcggt taactaattt aaggagtcta tcatgaaaac ttttatcaaa actttactcg
      781 ttgctgtaac tattctgttc tctgtcttcg ctactgcgaa acaagtaaaa ctgccaaata
      841 acatcaaata cgttaatact acagaagcgt tttcctgtac cgaaattgac ggtatgaatt
      901 gccagacaaa gaatcagttc aactataaag ataacagcta tgttttcgtg cttgaacgtg
      961 gtggtgcctg gtgctacgac tacactgtct cggtagttaa cctgaaaacc gggaaagcac
     1021 aaatgattga atacggggac aatcaactgt gctcaggtag caacaaaccg ttcttcgaaa
     1081 tcaaaaatgg cgtaccgacg gtaggagtca tcgacacatc cggaaaacct gtcggtgtag
     1141 cccaggacaa acttaaaatc taaggagtaa accatgcgtt tctctgcaca ttattaatat
     1201 caaaggtaaa tacagcccca gccattgtta ccggaaagcc gcttaatgcg tggctcgcca
     1261 gtgaaaggcc tctcagacaa taaaccttat tcatttcacc cgtcaggccg cgtcttctcc
     1321 gggagacgcg gcttttttca tttattgcac cagtaaatct taaccaccga taaggagcaa
     1381 agtatgcgat tagccagtcg ttttggttat gctgcaaacc agatacgccg tgaccgtccg
     1441 ctgacacatg aagaactgat acgccatgta cccagtattt ttggggaaga ccggcacacc
     1501 tcccgcagtg aacggtatgc gtacattccc accattaccg tcctggaaaa tctgcagcgg
     1561 gaaggctttc agccgttctt cgcctgccag acccgtgtgc gcgacccggg ccgccgggga
     1621 tacacaaaac acatgctgcg tctgcggcgg gccggagaga taaacggaga acatgtccct
     1681 gaaattattc tgctcaactc tcatgacggt acctccagct accagatgct gccgggttac
     1741 ttcaggttcg tctgccagaa cgggtgcgtc tgtggccagt ctctggggga agtgcgtgtt
     1801 ccacaccggg gaaatgtagt ggagaaagtt atcgaagggg cttacgaggt ggtgggcgtg
     1861 tttgaccgga tagaggagaa gcgtgatgcc atgcagtcgc tggtcctgcc gcaaccggca
     1921 cgccaggcgc tggcacaggc ggcactgact taccgttatg gtgacgaaca tcagcccgtc
     1981 accaccgccg acattctgac gccgcgacgc cgggaggatt acggtaagga cctgtggagc
     2041 gcatatcaga ccatccagga gaatatgctg aaaggcggga tttccggtcg cagtgcaaaa
     2101 ggaaaacgta tccatacccg ggccattcac agcattgata ccgacattaa gctcaatcgc
     2161 gcattgtggg tgatggcaga aacgatgctg gagagcctgc gctgatgccg tttccctgag
     2221 tgagggaatc attcagcttt cgttcccgaa aaacacacat ttatttgtta tttaaggagt
     2281 tatctcatga aaaccgtttc tcagaatacc cccacaattt attcagctac aacaccagag
     2341 aataatccgc ctcagttggt tgccagcctc gtccctgatg aacagcgcat cagcttctgg
     2401 ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg agccccgtgt cttcggctgg
     2461 atggaccgtc tgtgcgaaga ctactgcggt ggtatctgga atctgtacac cctgaacaac
     2521 ggcggggcat ttatggcccc cgaaccggat gatgatgatg acgaaacatg ggtactgttc
     2581 aatgccatga acggtaaccg cgctgaaatg agcccggaag ccgccggtat tgccgcctgt
     2641 ctgatgacgt acagccatca tgcctgtcgt acggagaatt atgccatgac ggtccattat
     2701 taccggttgc gggattacgc cctgcagcat ccggaaggca gcgccattat gcgcatcatt
     2761 gactgaaagg ggccggaata atgcaacaga tttcctttct gcccggagaa atgacgcccg
     2821 gcgagcgcag cctcattcaa cgggccctga aaaccctgga ccgccatctt catgaacccg
     2881 gtgtggcctt cacctccacc cgtgcagcac gggaatggct gattctgaac atggcgggac
     2941 tggagcgtga agagttccgg gtgctgtatc tgaacaacca gaatcagctg attgccggtg
     3001 aaaccctctt caccggcacc atcaaccgca cggaagtcca tccccgggaa gtgattaaac
     3061 gcgccctgta ccacaatgcc gctgccgtgg tactggcaca caatcacccg tccggtgaag
     3121 tcacacccag taaggcagac cggcttatca ccgaacgtct ggtacaggca ctgggcctgg
     3181 tggatatccg ggtgccggac catctgatag tcggtggcaa ccaggttttc tcctttgccg
     3241 aacatggtct gctttaaccc gtcacaacca catcacacct gttttcactt ttatcttctg
     3301 tcttcagagg tatcccatta tgaaaattat cacccgtggt gaagccatgc gtattcacca
     3361 acaacatccg acatcccgtc tttttccgtt ctgtaccggt aaataccgct ggcacggcag
     3421 tgctgaagcg tataccggtc gtgaagtgca ggatattccc ggtgtgctgg ccgtgtttgc
     3481 tgaacgccgt aaggacagtt ttggtccgta tgtccggctg atgagcgtca ccctgaactg
     3541 agtgggaatt ctgatgagca gaattatcgc cactaccgga ccattcttag ccgattttct
     3601 gtaaggattt tatcgtgtca gacacactcc ccgggacaac acttcccgac gacaatcacg
     3661 accgcccctg gtgggggctg ccctgcaccg tgacgccctg tttcggggca cgtctggtgc
     3721 aggagggtaa ccggttgcat taccttgccg accgcgccgg tatcagaggc ctgttcagcg
     3781 atgcagatgc gtaccacccg gaccaggcct ttccgctgct gatgaaacaa ctggaactca
     3841 tgctcaccag cggtgaactg aatccccgcc atcagcatac cgtcacgctg tatgcaaaag
     3901 ggctgacctg caaagccgat accctcagca gttgtggtta cgtttatctg gctgtttatc
     3961 cgacgcccga aatgaaaaat taactctcca gaatagcctt ctgctacggc ctggtgtttt
     4021 caccacgcca cttttccatt tttatatctg catatcagga aaatcttcag tatgaaaaca
     4081 ttacctgtat tacccgggca ggcggccagt tctcgcccgt ctcctgttga aatctggcag
     4141 atactgctgt cccgactgct ggaccagcac tatggcctca cactgaatga cacacctttt
     4201 gccgatgaac gtgtgattga gcagcatatt gaggcaggca tttcactgtg tgatgcggtg
     4261 aactttctcg tggaaaaata cgcgctggtg ctaccgacca gccgggattc agcgcctgta
     4321 cccgctctca gttaataaac agcatcgata tcctccgggc tcgcagggcg accggcctga
     4381 tgacccgcga caattacaga acggtaaata acattaccct gggtaagtat ccggaggcga
     4441 aatgatgaca ctggaagccg acagcgttaa cgtacaggcg ctggatatgg ggcacattgt
     4501 cgttgacatt gatggtgtta atatcactga actgattaat aaggccgctg aaaacggtta
     4561 ttcactccgc gtggtggatg accgtgactc taccgaaaca ccggcaactt atgccagccc
     4621 tcaccagttg ctgtaagaca atgcagtgat gcagggtata gtgccattaa ccacagaaac
     4681 agcactgttg tcctgtatta agcaggcaag agaagatgcc cttaccctgc gccatctgca
     4741 gcttctgcac cagaaccggt gaatctcact caacaggctt catttcccct gacgccagcc
     4801 tgaatacagc tggcgttttc atttataaac agaaaggaaa accgataata atggaatgga
     4861 cgccccgacc atgaagcaac ggtaggatat tactgataaa ccttccagtg gaggtgcgtt
     4921 atggccgttt taacgattag cattgacctg acaaaaaatg aattccaaat ccacggtctg
     4981 ggtaggaaca gaaaaatcta agctcagaaa acggattaaa gtaagcgtca acggagcacc
     5041 gtattgacgc ttatttattg gtgagaacta cgttccatgg caggagttcg tcaacacggt
     5101 tggagggcca ttccggcagt atgctcagaa tatggcgcag atacgcttcc ggatcgatac
     5161 cgttcagacg gcaggtgcct gcttttccgg caacaagcag gaatctcagc gctgaagcag
     5221 gaattagagg taaaaacgcc ctaccgggcc atgaatcacc cggtcattgg agtagtaacc
     5281 aaagcagatt tagccagcat ggaacaaata tctttggtga aatgttggtt acgggaagct
     5341 ggagcgcaca acgtgttagt aaccagtgcg gttaataaca atggagttac agaacttttt
     5401 gccttgctgc atacagaaga aggctgccgc taatttcgat aggttctcag atgagaatga
     5461 tgacggtgat acacagataa gaatcaatga gcaatgaggg ttgccgggca accctcattg
     5521 aataaaacag gaattacttc gcttcgccgt ttttcatttc gcccatagct ttcagctttt
     5581 tggagatatc gcggcgttct ttggagagct cggcattttt gatgatgtaa tcgtcaacgc
     5641 gatcttcata gtcacctttc atgctggcga tgatgccctg gattgcttca acgctcatcc
     5701 ctggcttgat gtaatcgctc aggttgtcca gcaacaggac gcgcttctgg ttgtcacgga
     5761 tctttttctc aacgtcctga atttcacgtt gcagtttgtt cttacgacgg aacagacgaa
     5821 caaattccag tacgtcctgg aatgaaggct tggtagtttc catttttata cccctgataa
     5881 tgtgagagtc ggattcgttt aagcaaccgc tattcgttag ggccaacatt actcatagct
     5941 gccgcagatg acaatgcttt tatcccttat cactatcata ccctttatcc ttgctgaatc
     6001 gaagcagcag caagatgatt ctgaagttca ggaattattt gcgcaaggcc cggcaaatca
     6061 gactcgatag caactcaagc tgatgtgccg tttccatgtt cagccacaca taaccataga
     6121 ttggtgtttc cacaaccttc aggtcatggt ggttattcag caactgacgc agctcttcca
     6181 cagcatcgtt caatttttcc gtattggcaa aaaccggctg cggattacct tcgtacagcg
     6241 catgaacaag gctcagcagt atttgctgca tcatgtgctg ggtatcacga agtttttgcg
     6301 cgttcaataa cacgaaatgg ctggggcgcg tggcccagta tgcattgatt tgcaactcca
     6361 gcatacaaac cagattgcgg ttaatggtct ggataccttc atatatcgat tttggaatac
     6421 gagtttcttt gctggcgggc gcaatcagcc cacgcatttt cacggcatcg gtcaggagtt
     6481 tttgtagatg gctttccaga cgtgggcgtt cgagtaagtt cggtgagaat gcagattgat
     6541 agacccgatt atactcggtc agacttttcg ccagttgaat gcgccagtgg atgaacgccc
     6601 gttgtggcca gataccggta aacaacattg ccagtaaaga gccgaggatc acatcgccgc
     6661 ttcgccataa cgccgtatca atttcacctg tcggggaacc cacaacaatt gccagcgtca
     6721 ccccaatcaa taatccttga tacggtttct tgcccagcgc cagccaaccg caaagaaaca
     6781 tcgccgccgc gcaccagact aacatcagcg gtaacgaaat taactccagt tgcagagcga
     6841 taagacctaa aatcgacccc aacaccgtac cgccaatacg ttcgaaggca cgagggacaa
     6901 cgttccccca gaacgagatt ggccccataa tcaccaccat ggtgaccagc ggccaggtgc
     6961 cttccgggat agtaaacagg cggataatga gaaaagtgag caggaatgcc agcgcgaccc
     7021 gagtaccatg cacaatgcgg taatggcggt atacccggat ttcaaacggg cttaatgact
     7081 tattggcacg cacgcagaaa ctccgttaat gaagattaaa agcgctcata acacatctgt
     7141 cggtcagaag gcgcgcctct gaattatccg acggacaaac cgggaacagt gtaatttccc
     7201 ggcaggagcg aaaaaattga aaggcgcatc ttactctttt cgctttcaaa aaaagagtgg
     7261 tcttcgcgtc aacacaccgc cctgagatga attagtgatg ttttggctgc accgcaacat
     7321 acatttcgat atcccagtac ccatcttccg cgccattgtt caagtaaacc tcgaagcagg
     7381 gctttggtaa catttcataa gcactgtcct gcaagaggct attaaagaac tgataccagg
     7441 gtttagcaaa atcatcaccg actacacgag ccaccgccac cgcatattga ccacctgaaa
     7501 tttctgtcag aatgacgccc tcactgtttt cggggagcgt aaagttattc ggtaccgtca
     7561 cgacggtgtc gcagcgtaat ttttcggcgg gtgtttcatc cggattgtcg taatagacag
     7621 caacccactc cttcggcaca atatttttgc tatctaccca catcatcaac tgctcaaaac
     7681 ctttctttac cgtctgttcc cacgggccaa cgagatggaa acctgcaacg gtacgtttat
     7741 cttcctgctt aatctcgtag ttcatgacgc ctccattatt actgttttta tatacagtat
     7801 agttgcaata ttaaaaccac aaggaatgag tgttgattat gcgagcagac tcgcactcct
     7861 gccagtctgc tgcaaaagaa aggtcaggcc ttatggtgga aataatcact caggcgagaa
     7921 aacatgctgc cttccccgac agattccagg gtaaccagcg gccagtgcgc cacctgttta
     7981 tcacggtcgt aaagttcaat ttcccctacc cgctgatggg cgctaattgg cgcggtgagc
     8041 tctttaccat caagggtata tttggctttg atatgtggaa tttcggcttt cggtagcacc
     8101 atccagaact cttgttccgt tcccagggcg atattttctt tatcgccata ccagatgcgt
     8161 tccgtaccga cctttttccc acggtgcaaa atttgcaccg tagtaaagtt ttgttgcccc
     8221 caacgcagta attttcttgc ctcttcctca cgaccttttg cactgtcagc ccccattacc
     8281 actgcaatga gacgacgctg cccatctaca gccgaagcaa tgagattaaa cccggcacca
     8341 gaagtatgac ccgttttcag gccgtcaaca ttcatggttt tatcccacaa taacccgtta
     8401 cggttttgct gggtgatacc gttccaggtg agacttttct cactgtacat atgataaaac
     8461 tcgggctcgc cgtggatgat agcgcgagaa agcacagcta aatcataagc cgagctatgc
     8521 tgacctggtg catccagacc atgcactgtt tcaaaatgcg tatccttgag atgcagcttc
     8581 tcggcatagt tgttcatcat ttcaacaaac tgccgttgcc caccggcaat atagtcagcc
     8641 agtgcaacac aagcgtcatt tccggaatcc acaattaaac cacggcttaa atcacgtacc
     8701 gatacgcgat cgccctcttt caaaaacatc agtgaagaac cgacaaacac tggattatct
     8761 ttcgcccacg catcgcgccc cacggtgaca atatcgtctg gcgtaatgcg atgactatcg
     8821 atagcgcgat ccacgacata gcccgtcatc agctttgtca ggctggcggg attgcgctgt
     8881 tgatgctcat tacccgcggt gaggatctga ccggtggtgt aatccatcaa tacccaggag
     8941 ccggcatgaa tctctggagg ctgaggtgaa aaaggaatgt tttccgccgc aaaaccagac
     9001 gataagttaa aaatgaacaa agaagcagca ataataagac ggcgtttcaa cagcaaaccc
     9061 tcaggagttt caaatagctg ttctttttac ggaaatactt atgaactggc tggaataaag
     9121 tgcaagaaaa tgtgactacc ctctcatttt tatctgacat gatctgttgc cactcgctgt
     9181 caaattgtcg cgctaaagct gattagcacg gtgatatttg atactctggc agacagcaga
     9241 aacaacggat ttaacctaat gatgaatgac ggtaagcaac aatctacctt tttgtttcac
     9301 gattacgaaa cctttggcac gcaccccgcg ttagatcgcc ctgcacagtt cgcagccatt
     9361 cgcaccgatg acgaattcaa tgtcatcggc gaacccgaag tcttttactg caagcccgcg
     9421 gatgactatt taccccagcc tggagcagta ttaattaccg gtattacccc gcaggaagct
     9481 cgggcgaaag gagaaaacga agccgcattt gccgcccgta ttcactcgct ttttaccgta
     9541 ccgaagacct gtattctggg ctacaacaat gtgcgtttcg acgacgaagt cacacgcaac
     9601 gttttttatc gtaatttcta cgatccttac gcctggagct ggcagcatga taactcgcgc
     9661 tgggatttac tggatgttat gcgtgcctgc tatgccctgc gcccggaagg aataaactgg
     9721 cctgaaaatg atgacggtct accgagcttt cgccttgagc atttaaccaa agcgaatggt
     9781 attgaacata gcaacgccca cgatgcgatg gctgatgtgt acgccactat tgcgatggcg
     9841 aaactggtaa aaacgcgtca accacgcctg tttgattatc tctttaccca tcgtaataaa
     9901 cacaaactga tggcgttgat tgatgttccg cagatgaaac ccctggttca cgtttccgga
     9961 atgtttgggg catggcgcgg caataccagc tgggtggcac cgctggcgtg gcatcctgaa
    10021 aatcgcaatg ccgtaattat ggtggatttg gcaggagata tttcgccatt actggaactg
    10081 gatagcgaca cattgcgcga gcgtttatat accgcaaaaa ccgatcttgg cgataacgcc
    10141 gccgttccgg ttaaactggt acatatcaat aaatgtccgg tgctggccca ggcgaatacg
    10201 ctacgcccgg aagatgccga ccgactggga attaatcgtc agcattgcct cgataacctg
    10261 aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg tggcgatatt cgcggaagcc
    10321 gaaccgttta cgccttcaga taacgtggat gcacagcttt ataatggctt tttcagtgac
    10381 gccgatcgtg cagcaatgaa aattgtgctt gaaaccgagc cgcgtaattt accggcactg
    10441 gatatcactt ttgttgataa acggattgaa aagctgttgt tcaattatcg ggcacgcaac
    10501 ttcccgggga cgctggatta tgccgagcag caacgctggc tggagcaccg ccgccaggtc
    10561 ttcacgccag agtttttaca aggctatgca gaagagattc agatgctggc gcagcagtat
    10621 gccgacgata aagaaaaagt ggcgctgtta aaagcgcttt ggcagtacgc ggaagagatt
    10681 gtctaatgtg aagcctccgc cgctggtacg gcggaggaca atgttgagtc aggctttttg
    10741 aacggtgatg ctccacgctg catcgccaat ctgctgataa tcggtgatgg catgcccttc
    10801 ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg caatcaaact caattaccaa
    10861 ctcatcgccg ctggccattt ctgccagtgc tgcttttgct tctatgagtg gaaacggaca
    10921 gacctgcgtc accacatcca gctttttaat cgccatcttt acaccttact taatttgccg
    10981 cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata cacaagccag gacgcagtcc
    11041 agactccgag aatcataaat acaaggccaa cccagccctg ccaggtcatc attgcagtca
    11101 taaccagtcc attaccgata gagcaaccac ctgcaatact ggcaccgaag cccatcagta
    11161 caccacctag cccgctacgt actgttgttt gtgcatcagc tgcgcgaacg cggaactcac
    11221 ggctcgcttt ggctgcaata aatgccccca cgaagatccc taacaccagg aaaacgcccc
    11281 agttaatgta tttcacgtca cccgcgacca gaaattgcag aatattggcc gttggggaag
    11341 tgatgcctaa cccaaacata cgtccggttg cttcactcag gggccaggct aaaagcgcaa
    11401 tcaaaccgat gagtacagct gtgacaaagg gatgccagcg tttttcaaac agaatatgag
    11461 cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag ttttggcttc ttcaactctt
    11521 tcatcaccac ccagagcgtt atcaccagca aaacggcaac caacggccac acagacaaat
    11581 taaaagtctc agcaatagag ttatgttcag tagtgtagtg ctgcaaggtt tgatttaaac
    11641 cactggcgtg tggagaacgc atcaccgcac tcatcaccat ataagtgaaa agcgcgatcc
    11701 aactgccgat caatccctca cccgcgcgat accaggtccc ggtggcacat ccacccgcca
    11761 gaacaattcc cagcccgaag agatacccac ctataacagt accaagccac gggaacgccc
    11821 cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa aacccccacg ctttgtaccg
    11881 aaattgcaat cagcaaggcg taaaacatgc gattattttt cgcgatatac atatcgcgaa
    11941 aaccaccagt caggcaaaaa cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa
    12001 ttagcccgct taatatcatt gaaaacataa attaattaac cagatgaatg ttaatgggga
    12061 aattattcat gactggtgga atgcagacca ataaccaaat tctctaaatt agaacaaatg
    12121 gttattaatg aggatttcga taaaaaagaa taaaaaaacc ggagccaaat gttcattcga
    12181 ctccggttta ttattagaaa tgatgattat gctacgtctt cgtactgcgg aaccgggtta
    12241 cggaagctct tggtgacgca agccaggtaa atcagaccga tagcagccca gatcagaccc
    12301 agaaccattg agctttcttc caggttaacc cacagcgcac caacggtcag cgcaccacac
    12361 atcggcagga acagatactg gaagtgatct ttcagcgtct tattacgctt ctcacggatc
    12421 cagaactgcg agatgaccga caggttaacg aaggtgaacg ccaccaacgc accaaagttg
    12481 atcagcgccg tagccattac caggtcgaag ttgattgcca gcagggcaat cgcgccaacc
    12541 aggatgatgt tcatcgccgg agtacgccat ttcgggtgta cataaccgaa gaaacttttc
    12601 gggaacacgc cgtcacgacc cattacgtac atcagacgcg ctacgcctgc gtgcgccgcc
    12661 ataccggacg ccagtacggt aatggtggag aagatcagcg caccgacctg gaacgcttta
    12721 cccgcaacgt acagcatgat ttcaggctgt gacgcatccg gatctttaaa gcgagagata
    12781 tccgggaagt acagctgcag gaagtaagtt gcaaagatga agatcatgcc gccgatcagc
    12841 gcggtcagga aaatcgcacg cgggatcacg cgctctgcat ctttggtttc ttccgacaga
    12901 ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc
    12961 gggataacat gcgcatcgcc agaccagaat ggacgagtgc tcgccagcgt accagcgcct
    13021 tcaccttcaa atacgccata aacaaccatg cccagaatca ccgcgatcag cactacctgc
    13081 aacacgacga ttacagtgtt gaagttcgct acggatttca gactacgcag gttaaaggcg
    13141 gtcataaagg ccaccagcgc caccacaaac atccacgatg ggatggaagg caccagagct
    13201 tcaaaataga ttttcgccag cagaatgttg atcatcggcg cgaacagata gtcgagcaga
    13261 gaagaccaac ccaccataaa gccgacagtc gggctaatgg atttctgggc gtaagtatat
    13321 gcagagccag cagaaggata gcggcgaacc agcttcccgt agctcagagc cgtaaacagg
    13381 atcgcaatca atgcgaacgc ataggctgtc ggcacatgac catccgtgag gcctgaaacg
    13441 ataccaaaag tatcaaacag cgtcatcggc tgcatatagg caagacccat cattacaacc
    13501 ggaactaacg taagcgtttt acgcaattcc acgcgagagg tgtttggagt aacgttatgc
    13561 gacatggtca ttctccttta cggtgataac cgtcgcgtaa gcaaaaaatt gccccatttt
    13621 tttggattcc tcagcgacaa caactgtcga tttttagtaa atatctatcc ggtacgaagc
    13681 ccggcctctt ggtatgaatg attggtttga agcaaaaaat aaccgacgct gatgaaacgt
    13741 cggtttttag tcattttttg acagcggcgc attgtgccta aatggggggg aaatgacaag
    13801 agaatgagag gcttgtcata ataatttttc tttaaatggc tgattttccg tcatcagatt
    13861 ttgcataaac accggcgatc gcactatttg ctaaaatttc atcccgccac cagccgctgg
    13921 caagtcctgc ggtctgttca ttccagccaa tccagacggg ttcaaagaga gtttgggcga
    13981 ccactttctt ctcgattaac gcgccactat cgagaaaacg ttgcgccaga taacggggta
    14041 aatagccgca gccgaggccg ctaatttgca gctccagctt ggttttaaaa tcgaaaacgg
    14101 taatggcttc ttgttcgtcg agaagttgtg aggccgtaga agcgcctgcc tgcgcagtgt
    14161 cccccaccac aatcgcccga tatcgtttaa tgatacgacg gtttaatggt tcttcttcct
    14221 gggctaacgg atgatgaggc gcgaccgcaa agacttgttc cagatcgccc aaccgcgaga
    14281 aaccaaactc actggaagat ggtggctcat gcatcgcgcc aacaataata tccgctcgcc
    14341 cctgagtcag ggcatcccat gaaccaccga gtacgccatt gataaacttc aggcgcgtca
    14401 cgttatgatg ttgataaaaa gcttcaatga gtggcgcaag cagagaaaaa ggaaacgtgt
    14461 cgtccacgcc aatcaccagc tcgttttccc agccttcatg cagtttgatc gcctgctttt
    14521 ccagttctcg cacggtatgc agaacttccc gccccttctc taataacatt tttccggtac
    14581 gggtgaattt agcgcggtga ccgctacgat caagcaattg aatattaagg tcgctttcca
    14641 gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta gagtttagcc gacgccgccg
    14701 caaaactgcc ttctttttct aaggcatcga ggatcatcaa cacatccagc agtggtttca
    14761 tacacgcccc cttgcagtgc atgcggcgtc ctgctggcgt gatcgtttac tccagcggca
    14821 ttaccagcgg atcgggatac tggtattcaa atcccagttc attacaaatc cgactgccat
    14881 caataatctt acctttgccg ctgtccagac tatttctgaa ttgcggaggt tccagcccca
    14941 gtaaacgggc catctgcgga tagaaaacat tacgcgcagg gtgagctggc gcacatatat
    15001 tatagatgtg tccgccttta ggtgcctgta acaacagagt gatagcgcca ataacatctt
    15061 ctaaatggac taaattaaca ccatgttcac catcaggcgc ggtttttccg gcaaagaagc
    15121 gtccgggatg acgccccggt ccgaccaggc ccgcaagacg cagaatatcg accgaagtac
    15181 cgggtaaatt gtgcagccag tcttcgagtt cttctaatac tcgtccactg ttggtcaccg
    15241 gattacgcgg ggtggtttct ttcaccgtgc cttgcgcgtc gccatagaca gacgttgaac
    15301 tggtaaaaat aattcgggga atacgatggg ccagcgcgct atccactaac tcttgtaccg
    15361 cttgtaaata gaactcatcg ccggggccgc tacgacgggc cggaagcgta atgaccagcg
    15421 catcggcatc catcagggca tccagatcgt cagaatcgca aactaactca ggctccatgc
    15481 gaagcagata gctatcaatg ccactcattc gggccgcttc gacaccatct tgtgtggttt
    15541 tactcccggt gacttgccag cctcgcgctg aaagtgacat cgccagcggc atgcctaacc
    15601 accctaaccc gacaattgcg acctttttca tccgttatct cctgatacac cttagatcta
    15661 taaggctacg ctagcgtatc aaaactgaca attcattcta tgaatgaaat cgaaactgtt
    15721 agcaaatata ccattgcctg atgcgctccg cttattagtc ctacaaaaca tcaatatatt
    15781 gaatttaggc aacatttgta gaccagataa ggcattcacg ccgcatccgg caggaacaaa
    15841 gcccacttta gttacagaac acaatgttct tgagggtttt aatgacatat tttcacgatt
    15901 acttttttaa cctagtcact cttagccccc tcctttcccc gctcattcat taaacaaatc
    15961 catcgccata aaatatataa aaaagccctt gctttctaac gtgaaagtgg tttaggttaa
    16021 aagacatcag ttgaataaac attcacagag acttttatga cacgcgttca atttaaacag
    16081 caccatcatc accatcatcc tgactagtct ttcaggcgat gtgtgctgga agacattcag
    16141 atcttccagt ggtgcatgaa cgcatgagaa agcccccgga agatcacctt ccgggggctt
    16201 ttttattgcg cggttgataa cggttcagac aggtttaaag aggaataaga aaatgacaga
    16261 caacactcgt ttacgcatag ctatgcagaa atccggccgt ttaagtgatg actctcgcga
    16321 attgctggcg cgctgtggca ttaaaatcaa tcttcacacc cagcgcctga tcgcgatggc
    16381 agaaaacatg ccgattgata ttctgcgcgt gcgtgacgac gacattcccg gtctggtaat
    16441 ggacggcgtg gtagatcttg ggattatcgg cgaaaacgtg ctggaagaag agctgcttaa
    16501 ccgccgcgcc cagggtgaag atccacgcta ctttaccctg cgtcgtctgg atttcggcgg
    16561 ctgccgtctt tcgctggcaa cgccggttga tgatgcctgg gacggcccgc tctccttaaa
    16621 cggtaaacgt atcgccacct cttatcctca cctgctcaag cgttatctcg accagaaagg
    16681 catctctttt aaatcctgct tactgaacgg ttctgttgaa gtcgccccgc gtgccggact
    16741 ggcggatgcg atttgcgatc tggtttccac cggtgccacg ctggaagcaa acggcctgcg
    16801 cgaagtcgaa gttatctatc gctcgaaagc ctgcctgata caacgcgatg gcgaaatgga
    16861 agaatccaaa cagcaactga tcgacaagct gctgacccgt attcagggtg tgatccaggc
    16921 gcgcgaatca aaatacatca tgatgcacgc accgacccaa cgtctggatg aagtcatcgc
    16981 cctgctgcca ggtgccgaac gcccaaccat tctgccgctg gcgggtgatc aacagcgcgt
    17041 agcgatgcac atggtcagta gcgaaaccct gttctgggaa acgatggaaa aactgaaagc
    17101 gctgggtgcc agttcaatcc tggtcctgcc gattgagaag atgatggagt gatcgccatg
    17161 agctttaaca caatcattga ctggaatagc tgtactgcaa agcaacaacg ccagctgtta
    17221 atgcgcccgg cgatctccgc ttctgaaagc attacccgca ctgttaacga tattctcgat
    17281 agcgtgaaag cacgcggtga tgacgccctg cgggaatata gcgcgaagtt tgataaaacc
    17341 acggttaccg cactgaaggt gtctgctgag gaaattgccg ccgccagcga acgcctgagc
    17401 gacgagctaa aacaggcgat ggcggtggca gtaaagaata ttgaaacctt ccacactgcg
    17461 caaaaactgc cgccggtaga tgtagaaacg cagccaggcg tgcgttgcca gcaagtcacg
    17521 cgcccggtag cttcagttgg gttgtatatt cctggcggct ccgccccgct cttctcaacg
    17581 gtattaatgc tggcaactcc ggcgcgtatt gcgggctgta aaaaagtggt gttgtgctca
    17641 ccgccgccga ttgccgatga gatcctttat gcggcgcagc tgtgcggtgt gcaggacgtg
    17701 tttaacgtcg gcggcgcaca ggccattgcc gcgctggcgt ttggtacgga atctgtgccg
    17761 aaagtggaca aaatcttcgg gccgggtaac gcctttgtca ccgaggcaaa acgtcaggtg
    17821 agccagcgtc tggacggtgc ggcgatcgat atgcccgcag gcccgtcgga agtgttggtc
    17881 attgccgaca gcggcgcaac gccggatttc gtggcttctg atttgctttc tcaggctgaa
    17941 cacggcccgg actcacaggt gattttactg acgcctgacg ctgatatggc gcatcaagtt
    18001 gccgaagccg tcgaacgcca gttagcagaa ctgccgcgtg ccgaaaccgc ccgccaggca
    18061 ctgaacgcca gccgcctgat cgtgactaaa gatttagcgc agtgcgtgga gatctccaac
    18121 cagtacggcc cggagcacct gatcattcag acccgcaacg cccgcgaact ggtcgatggc
    18181 atcaccagcg ccggttcggt atttcttggt gactggtcac cggaatcggc aggtgattac
    18241 gcctccggaa ccaaccatgt tctaccgact tacggctaca ccgccacctg ttccagcctc
    18301 ggactggcgg atttccagaa gcggatgacc gtgcaggaac tgtcgaaagt aggtttctcc
    18361 gcgctggctt cgaccattga aacactggcc gccgccgagc gcctgaccgc ccacaaaaat
    18421 gccgttactt tgcgtgttaa cgcccttaag gagcaagcat gagcaccgtg actattaccg
    18481 atttagcgcg tgaaaacgtc cgcaacctga cgccgtatca gtcggcgcgt cgtctgggcg
    18541 gtaatggcga cgtctggctg aacgccaacg aatatcccac agccgtggag tttcagctta
    18601 ctcagcaaac gctcaaccgc tacccggaat gccagccgaa agcggtgatt gaaaattacg
    18661 cgcaatatgc aggcgtaaaa ccggaacagg tgctggtcag ccgtggcgcg gacgaaggta
    18721 ttgaactact gattcgcgct ttttgcgaac cgggtaaaga cgccatcctc tactgcccgc
    18781 caacgtacgg catgtacagc gtcagcgctg aaaccattgg cgtcgagtgc cgcacagtgc
    18841 cgacgctgga aaactggcaa ctggacttac agggcatttc cgacaagctg gacggcgtaa
    18901 aagtggtcta tgtttgcagc cccaacaacc caaccgggca actgatcaat ccgcaggatt
    18961 ttcgcaccct gctggagtta acgcgcggta aggcgattgt ggttgccgat gaagcctata
    19021 tcgagttttg cccgcaggca tcgctggctg gctggctggc ggaatatccg cacctggcta
    19081 ttttgcgcac actgtcgaaa gcttttgctc tggcgggcct tcgttgcgga tttacgctgg
    19141 caaacgaaga agtcatcaac ctgctgatga aagtgattgc cccctacccg ctctcgacgc
    19201 cggttgccga cattgcggcc caggcgttaa gcccgcaggg aattatcgcc atgcgcgaac
    19261 gggtggcgca aattattgca gaacgcgaat acctgattgc cgcactgaaa gagatcccct
    19321 gcgtggagca ggtttttgac tctgaaacca actacattct ggcgcgcttt aaagcctcca
    19381 gtgcagtgtt taaatctttg tgggatcagg gcattatctt acgtgatcag aataaacaac
    19441 cctctttaag cggctgcctg cgaattaccg tcggaacccg tgaagaaagc cagcgcgtca
    19501 ttgacgcctt acgtgcggag caagtttgat gagtcagaag tatcttttta tcgatcgcga
    19561 tggaaccctg attagcgaac cgccgagtga ttttcaggtg gatcgttttg acaaactcgc
    19621 ctttgaaccg ggcgtgatcc cgcaactgct gaagctgcaa aaagcgggct acaaactggt
    19681 gatgatcact aatcaggatg gtctgggaac acaaagtttc ccgcaggcgg atttcgatgg
    19741 cccgcacaac ctgatgatgc agatattcac ctcgcaaggc gtacagtttg atgaagtgct
    19801 gatttgtccg cacctgcccg ccgatgagtg cgactgccgt aaaccgaaag taaaactggt
    19861 ggagcgttat ctcgctgagc aagcgatgga tcgcgccaac agttatgtga ttggcgatcg
    19921 cgcgaccgat attcaactcg ctgaaaacat gggcattaat ggtttacgct acgaccgcga
    19981 aaccctgaac tggccaatga ttggcgagca actcaccaga cgtgaccgtt acgctcacgt
    20041 agtgcgtaat accaaagaga cgcagattga cgttcaggtg tggctggatc gtgaaggtgg
    20101 cagcaagatt aataccggcg ttggcttctt tgatcacatg ctggatcaga tcgccaccca
    20161 cggcggtttc cgtatggaaa tcaacgtcaa aggcgacctc tatatcgacg atcaccacac
    20221 cgtcgaagat accggcctgg cgctgggcga agcgttaaaa attgccctcg gcgataaacg
    20281 cggtatttgc cgctttggtt ttgtgctgcc gatggacgaa tgccttgccc gctgcgcgct
    20341 ggatatctct ggtcgcccgc acctggaata taaagccgag tttacctacc aacgcgtggg
    20401 cgatctcagc accgagatga tcgagcactt cttccgttcg ctctcttaca ccatgggcgt
    20461 gacgctccac ctgaaaacca aaggtaaaaa cgatcatcac cgtgtagaga gcctgttcaa
    20521 agcctttggt cggaccctgc gccaggccat ccgcgtggaa ggcgataccc tgccctcgtc
    20581 gaaaggagtg ctgtaatgaa cgtggtgatc cttgatactg gctgcgccaa cctgaactcg
    20641 gtgaagtctg ccattgcgcg tcacggttat gaacccaaag tcagccgtga cccggacgtg
    20701 gtgttgctgg ccgataaact atttttaccc ggcgttggca ctgcacaagc agcgatggat
    20761 caggtgcgtg agcgcgagct gtttgatctc atcaaagcct gtacccaacc ggtgctgggc
    20821 atctgcttag ggatgcaact gctggggcgg cgcagtgaag agagcaacgg cgtcgatttg
    20881 ctgggcatca tcgacgaaga cgtgccgaaa atgaccgact ttggtctgcc gctgccgcat
    20941 atgggctgga atcgcgttta cccgcaggca ggcaaccgcc tgtttcaggg gattgaagac
    21001 ggcgcgtact tttactttgt tcacagctac gcaatgccag tcaatccgtg gaccatcgcc
    21061 cagtgtaatt acggcgaacc attcaccgcg gcggtacaaa aagataactt ctacggcgtg
    21121 cagttccatc cggagcgttc tggtgccgct ggcgctaagt tgctgaaaaa cttcctggag
    21181 atgtgatgat tattccggca ttagatttaa tcgacggcac tgtggtgcgt ctccatcagg
    21241 gcgattacgg caaacaacgc gattacggta acgacccgct gccgcgctta caggattacg
    21301 ccgcacaagg tgccgaagtg ctgcatttgg tggatctgac cggggcaaaa gatccggcaa
    21361 aacgtcaaat cccgctgatt aaaaccctgg tcgctggcgt taacgttccg gtgcaggttg
    21421 gcggcggcgt gcgtagcgaa gaagacgtgg cggcgttact ggaagctggg gttgcgcgcg
    21481 tggtggttgg ctccactgcg gtgaaatcac cagaaatggt gaaaggctgg tttgaacgtt
    21541 tcggtgccga tgccttagtg ctggcgctgg atgtccgtat tgacgagcaa ggcaacaagc
    21601 aggtggcggt cagcggctgg caagagaatt cgggcgtgtc actggaacaa ctggtggaaa
    21661 cctatctgcc cgtcggcctg aaacatgtgc tgtgtaccga tatctcgcgc gacggcacgc
    21721 tggcaggctc taacgtctct ttatatgaag aagtgtgcgc cagatatccg caggtggcat
    21781 ttcagtcatc cggcggcatt ggcgacatta acgatgttgc tgccctgcgt ggcactggcg
    21841 tgcgcggcgt aatagttggt cgtgcattac tggaaggtaa attcaccgtg aaggaggcca
    21901 tctcatgctg gcaaaacgca taattccatg tctcgacgtt cgtgatggtc aggtggtaaa
    21961 aggcgtacag tttcgcaacc atgaaatcat tggcgatatc gtgccgctgg caaaacgcta
    22021 cgccgaagaa ggtgcagacg aactggtgtt ctacgatatc accgcttcca gcgatggccg
    22081 tgtggtagat aaaagttggg tatctcgcgt ggcggaagtg atcgacattc cgttctgcgt
    22141 ggcgggtggg attaagtctc tggaagatgc cgcgaaaatt ctttcctttg gcgcggataa
    22201 aatttccatc aactcccctg cgctggcgga cccgacgtta attactcgcc tggcggatcg
    22261 atttggcgtg cagtgtattg tggtgggtat tgatacctgg tacgacgctg aaaccggtaa
    22321 atatcatgtg aatcaatata ccggcgatga aagccgcacc cgcgtcactc agtgggaaac
    22381 gctcgactgg gtacaggaag tgcaaaaacg cggtgccgga gaaatcgtcc tcaacatgat
    22441 gaatcaggac ggcgtgcgta acggttacga cctcgaacaa ctgaaaaaag tgcgtgaagt
    22501 ttgccacgtc ccgctgattg cttccggtgg cgcgggcacc atggaacact tcctcgaagc
    22561 cttccgcgat gccgacgttg acggcgcgct ggcagcttcc gtattccaca aacaaataat
    22621 caatattggt gaattaaaag cgtacctggc aacacagggc gtggagatca ggatatgtta
    22681 acagaacaac aacgtcgcga actggactgg gaaaaaaccg acggactgat gccggtgatt
    22741 gtgcaacacg cggtatccgg cgaagtgtta atgctgggct atatgaaccc ggaagcctta
    22801 gacaaaacca tcgaaagcgg caaagtcacc ttcttctcgc gcactaaaca gcgactgtgg
    22861 accaaaggcg aaacgtcggg caatttcctc aacgtagtaa atattgcgcc ggactgcgac
    22921 aacgacacgc tactggtact ggcgaatcct atcggaccaa cctgccacaa aggcaccagc
    22981 agctgcttcg gcgacaccgc tcaccagtgg ctgttcctgt atcaactgga acaattgctc
    23041 gccgaacgca aatctgccga cccggaaacc tcctacaccg ccaaactgta tgccagcggc
    23101 accaaacgca ttgcgcagaa agtgggcgaa gaaggcgtgg aaaccgcact ggccgcgact
    23161 gtacatgacc gctttgagct gaccaacgag gcgtctgact tgatgtatca cctgctggta
    23221 ttgttgcagg atcaggatct ggatttaaca acggtgattg agaacctgcg taaacgtcat
    23281 cagtgagttg cggggtaagc ggatgcgata ttgttgtcgc atccggcaaa aaaaacgggc
    23341 aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg cgttgtaatt
    23401 acgcagagcg ttacgcccca gcacaatccc cgcgccaacc atgccgccca gcagcaccgc
    23461 cagaatcaag gtgattgcct ttttcggact atcgcgacga ataggtaacg tcggtttcat
    23521 tacatagcgg taagcatgaa tatcaagatc atcaaccttt aagttttcaa tatccaacag
    23581 gttttgacgt gtctgatagt agtttggtga gaacaccaac ggacgggtcg cctcatgctt
    23641 aatcatcgac tccagcgctt cgctccccaa aaggaacaac gtatcttgcg tcacatcttc
    23701 agtctgctga acctgtggct ttgccacctg cgcctgattc gcatactgca acgcttcctg
    23761 aatctgacgg atacgcagct ctttctgctc ctgcgcaact atttcctgcg ttcttaaaga
    23821 gtcctgcaag tttttccgtc ccagagcaat gttgtctttg agatcctttt ctagctcttg
    23881 attcacttta tcatcaactt gctgaatgta ttgggccaac ttcatttgtg ctccctctgc
    23941 agtttgccca acataagaaa cagtcaatgg taattgctgg ttcttaacag aaggttcgat
    24001 ggtaagtttt tctggttctt cctgattatc cagcgtttct gctaatgctg agaaggcaga
    24061 actgaagcga ccaatcaacg tctcctgcaa atccgatact ttcggtgcag cctgaccata
    24121 gataacattc atggcattgt tatagccagc aatttgcccc acgtcgggct gagtgataat
    24181 tgctgttgac gtccatttct ccttcgctac tgccaaataa ccaatagcca gagcaatagc
    24241 cacaatgacg gaaatgatga ttgtcatctt gccacgccac aactgcacta gtaaatcaat
    24301 caaatcaatc tgttccgggt catggttttg cccagaaaca ttattatttt ctactctcat
    24361 cattacccta actgacggaa atttccaagt ccgcatagtt tagcgataat ccctgtgaat
    24421 gctataggat aagtgataaa aataatgaat tattgcggag gtaacaaaag acaatcaggg
    24481 cgtaaatagc cctgataaca agatgttagt cgctgccaaa gagatcgcgg gtgtagacct
    24541 tatctgccac atccctaagc tcttctgcca tacggttgga aataatgacg tcggcttgtt
    24601 gtttgaaggt ggcgagatca cgttccaggc gagagttgaa gaatgagtct tctttcatca
    24661 ctggctcgta gatgattact tcaacacctt tcgccttgat acgcttcata atcccctgaa
    24721 tagaagacgc acggaagtta tctgaaccgc tcttcataat cagacgataa atacctacca
    24781 cttgcggctt gcgtgacaaa atggcatcgg caataaaatc tttacgcgtg cggttagcat
    24841 cgacaattgc cgagatcagg ttattcggca cagactggta gttcgccagt aactgcttgg
    24901 tatctttcgg caaacaataa ccaccataac caaacgacgg attgttgtaa tggttgccaa
    24961 tacgtgggtc gagacaaacg ccttcgatta tttgacggga attcagacct aaactttctg
    25021 cgtagctatc cagttcgtta aagtacgcca cgcgcatcgc gaggtaggtg tttgcaaaaa
    25081 gtttaatcgc ttctgcttca gtggagtcgg taaacagggt tgggatattt tgcttaatcg
    25141 cgccttcctg taataacgca gcaaaacgtt ctgcgcgttc cgaacgctca ccgattacaa
    25201 tacgtgacgg atgaaggtta tcgtaaagag ctttaccttc gcggagaaat tccggagaga
    25261 aaataatatt ttcagtacga tatttcttat gcatcgcttc ggtaaaacca acgggaaccg
    25321 ttgatttgat gaccataacc gcataaggat ttatctcaac tacgt
//
LOCUS       DAZFDC010000064        25124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFDC010000064 DAZFDC010000000
VERSION     DAZFDC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.064"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            265..2940
                     /gene="adhE"
                     /locus_tag="ACHJ48_002705"
                     /gene_synonym="adhC"
     CDS             265..2940
                     /gene="adhE"
                     /locus_tag="ACHJ48_002705"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO4708236.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(3241..3858)
                     /gene="tdk"
                     /locus_tag="ACHJ48_002706"
     CDS             complement(3241..3858)
                     /gene="tdk"
                     /locus_tag="ACHJ48_002706"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO4708237.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            4462..4875
                     /gene="hns"
                     /locus_tag="ACHJ48_002707"
     CDS             4462..4875
                     /gene="hns"
                     /locus_tag="ACHJ48_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO4708238.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(5019..5927)
                     /gene="galU"
                     /locus_tag="ACHJ48_002708"
     CDS             complement(5019..5927)
                     /gene="galU"
                     /locus_tag="ACHJ48_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO4708239.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(6129..7142)
                     /gene="rssB"
                     /locus_tag="ACHJ48_002709"
     CDS             complement(6129..7142)
                     /gene="rssB"
                     /locus_tag="ACHJ48_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO4708240.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(7234..8139)
                     /gene="rssA"
                     /locus_tag="ACHJ48_002710"
     CDS             complement(7234..8139)
                     /gene="rssA"
                     /locus_tag="ACHJ48_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO4708241.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            8252..8710
                     /gene="ychJ"
                     /locus_tag="ACHJ48_002711"
     CDS             8252..8710
                     /gene="ychJ"
                     /locus_tag="ACHJ48_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO4708242.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            8760..9602
                     /gene="purU"
                     /locus_tag="ACHJ48_002712"
     CDS             8760..9602
                     /gene="purU"
                     /locus_tag="ACHJ48_002712"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO4708243.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            9762..9846
                     /locus_tag="ACHJ48_002713"
     tRNA            9762..9846
                     /locus_tag="ACHJ48_002713"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9796..9798,aa:Tyr,seq:gta)
     gene            9891..10021
                     /locus_tag="ACHJ48_002714"
     ncRNA           9891..10021
                     /ncRNA_class="other"
                     /locus_tag="ACHJ48_002714"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10056..10140
                     /locus_tag="ACHJ48_002715"
     tRNA            10056..10140
                     /locus_tag="ACHJ48_002715"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10090..10092,aa:Tyr,seq:gta)
     gene            10187..10318
                     /locus_tag="ACHJ48_002716"
     ncRNA           10187..10318
                     /ncRNA_class="other"
                     /locus_tag="ACHJ48_002716"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10365..10496
                     /locus_tag="ACHJ48_002717"
     ncRNA           10365..10496
                     /ncRNA_class="other"
                     /locus_tag="ACHJ48_002717"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(10680..11357)
                     /gene="narI"
                     /locus_tag="ACHJ48_002718"
     CDS             complement(10680..11357)
                     /gene="narI"
                     /locus_tag="ACHJ48_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4708244.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(11357..12067)
                     /gene="narJ"
                     /locus_tag="ACHJ48_002719"
     CDS             complement(11357..12067)
                     /gene="narJ"
                     /locus_tag="ACHJ48_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4708245.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(12064..13602)
                     /gene="narH"
                     /locus_tag="ACHJ48_002720"
     CDS             complement(12064..13602)
                     /gene="narH"
                     /locus_tag="ACHJ48_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4708246.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(13599..17342)
                     /gene="narG"
                     /locus_tag="ACHJ48_002721"
     CDS             complement(13599..17342)
                     /gene="narG"
                     /locus_tag="ACHJ48_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO4708247.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(17735..19126)
                     /gene="narK"
                     /locus_tag="ACHJ48_002722"
     CDS             complement(17735..19126)
                     /gene="narK"
                     /locus_tag="ACHJ48_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO4708248.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            19465..21261
                     /gene="narX"
                     /locus_tag="ACHJ48_002723"
     CDS             19465..21261
                     /gene="narX"
                     /locus_tag="ACHJ48_002723"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO4708249.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            21254..21904
                     /gene="narL"
                     /locus_tag="ACHJ48_002724"
     CDS             21254..21904
                     /gene="narL"
                     /locus_tag="ACHJ48_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO4708250.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(21905..23299)
                     /gene="ychO"
                     /locus_tag="ACHJ48_002725"
                     /gene_synonym="iatA"
     CDS             complement(21905..23299)
                     /gene="ychO"
                     /locus_tag="ACHJ48_002725"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO4708251.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            23485..23838
                     /gene="ychN"
                     /locus_tag="ACHJ48_002726"
     CDS             23485..23838
                     /gene="ychN"
                     /locus_tag="ACHJ48_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO4708252.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(23882..24577)
                     /gene="chaC"
                     /locus_tag="ACHJ48_002727"
     CDS             complement(23882..24577)
                     /gene="chaC"
                     /locus_tag="ACHJ48_002727"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO4708253.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     RPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(24738..24968)
                     /gene="chaB"
                     /locus_tag="ACHJ48_002728"
     CDS             complement(24738..24968)
                     /gene="chaB"
                     /locus_tag="ACHJ48_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO4708254.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
BASE COUNT         6349 a         6603 c         6082 g         6090 t
ORIGIN      
        1 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
       61 gagtaatctt gcttacgcca cttggaagtg acgcattaga gataataact ctaatgttta
      121 aactctttta gtaaatcaca gtgagtgtgt gcgcgagtaa gcttttgatt ttcatcggtt
      181 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
      241 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
      301 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
      361 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
      421 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
      481 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
      541 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
      601 accactaacc cgacttcaac agctatcttc aaatcgctga tcagtctgaa gacccgtaac
      661 gccattatct tctccccgca cccgcgtgca aaagatgcga ccaacaaagc ggctgatatc
      721 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
      781 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
      841 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggcgta
      901 ggcgcgggca acactccggt tgttatcgat gaaactgctg atatcaaacg tgcagttgca
      961 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
     1021 gttgtcgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
     1081 ctgttgcagg gtaaagagct gaaagctgtt caggacgtta tcctgaaaaa cggtgcgctg
     1141 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
     1201 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
     1261 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
     1321 gtagaaaaag cagagaaact ggttgctatg ggtggtatcg gtcatacctc ttgcctgtac
     1381 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacggct
     1441 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
     1501 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
     1561 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
     1621 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
     1681 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
     1741 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
     1801 ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
     1861 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
     1921 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
     1981 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
     2041 attgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
     2101 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
     2161 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
     2221 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
     2281 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
     2341 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcgactat cgcgggtatc
     2401 gcgtttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
     2461 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
     2521 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
     2581 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
     2641 gctaagatcg agaaactgct ggcatggctg gaaacgctga aggctgaact gggtattccg
     2701 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
     2761 tctgaagatg cattcgatga ccagtgcact ggcgctaacc cgcgttaccc gctgatctcc
     2821 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
     2881 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
     2941 tcagtagcgc tgtctggcaa cataaacggc cccttctggg gccgtttttt tgtttaccca
     3001 aagcaacttt tccataaacc gacagcatta gccttcatca tatttgcgac gatgtataac
     3061 gcctaaacac agggatattg tactttacag gtcacaagtc aacgtcggtg cttaagagcc
     3121 ctgtgaggcg tatagcggcg ttaaaaactg ccgagaaggg tgtatagccc ggaagaagtg
     3181 cgtaaaacga actgacagga taaaagtgcc ctgctcaccc tgtcagtaaa gaaattctta
     3241 ttaatcgtgg cgatgccttt cctgaatagc cgttaatgag ccgacttgta acgcctcttt
     3301 atagtgttta cggcatacag aaacgtatcg ttcattacca ccaataacca cctgctcacc
     3361 ttcgttataa ggtctgcctg cttgatcaag acgcagcacc atgcttgctt tacggccaca
     3421 aaaacagatg gtttttaatt caaccagttt gtcggaccat gccagtaagt attggctgcc
     3481 aataaataat tcacctcgaa aatcggtacg taaaccataa caaagtacgg gtatatcgag
     3541 ttgatcgaca acctccgata attcatatac ttgttgtctg gttaaaaact ggcattcatc
     3601 aaccagtacg caatgaattg cctgctgctc atgttccgca cgaatctcat caaataatga
     3661 tgaattttgg ttaaataatt ttgcaggcga tgacaaacct atacgcgaac tgactttccc
     3721 ggcaccaaag cgatcatcaa tttctgccgt atatacgaca gtgcgcatgc cgcgttcctg
     3781 gtaattgtat gaagattgca acaatgctgt agacttaccc gcattcattg cggaatagta
     3841 gaaatatagc tgtgccatca gccacaggcc ctcaatgata tgaaataaag ttggctggag
     3901 tttatcataa ttcgtcagtt tttcagctca acagtatgca cagaagtggt atatgcgttc
     3961 tcccttacga agccttgcat aatccttctg agctatcatt acaactgcct cgcttgttat
     4021 aagcgggtaa atgactgctg gtaactattc acaatcttta acctgttgcg caagtaatag
     4081 ccctctgttg acctccagga gatagtgcaa tactaagtcc ctgctcttat tgcgactgtt
     4141 ctacttttca tcattcgctt aatagggaat tctcgtaaac acgactaata cagaagactg
     4201 aaaggtcgtc agcctacgat aatctcccca taaaatgtga catgaatcag gaagttttaa
     4261 cctcacgtgc tgcgaaatca tcggtacaaa tagggctata tgccgcgtct tttctggcta
     4321 attttatgaa aagatattta ttggcggcac aaaataaaga acaattttga attccttaca
     4381 ttcctggcta ttgcacaact gaatttaagg ctctattatt acctcaacaa accaccccaa
     4441 tataagtttg agattactac aatgagcgaa gcacttaaaa ttctgaacaa catccgtact
     4501 cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg aagaaatgct ggaaaaatta
     4561 gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg ctgctgctga agttgaagag
     4621 cgcactcgta aactgcagca atatcgcgaa atgctgatcg ctgacggtat tgacccgaac
     4681 gagctgctga atagccttgc cgccgttaaa tctggcacca aagctaaacg tgctcagcgt
     4741 ccggcaaaat atagctacgt tgacgaaaac ggcgaaacta aaacctggac tggccagggc
     4801 cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag gtaaatccct cgacgatttc
     4861 ctgatcaagc aataatcttt tgtagattgc acttgcttaa aatcccgcca tcggcgggat
     4921 tttttattgt ccggtttaag acaatttaat aagaataagc tataaaaaaa cggcgtcgat
     4981 tgctcaacgc cgtttcgtgg ataacaccga tacggatgtt acttcttaat gcccatctct
     5041 tcttcaagcc aggctttaaa ttccgtgcca agggtgttat gacgaatacc gtattcaacg
     5101 aaggcctgca tgtaacctaa tttattaccg cagtcatggc tcttcccttt catatgatag
     5161 gcttcaaccg tttctttttc gattagcata tcaattgcgt cggtgagctg aatttcatca
     5221 ccagctcccg gaggggtttt tgccagcaac ggccaaatat ccgcactaag tacgtaacga
     5281 cccacaatag cgagattaga cggcgcaaca tccgcttttg gtttttcaac cacgccaacc
     5341 atcggtacgc tttcacccgg cgctaattca acgcctttgc aatccacaac gccatatgcg
     5401 gtcacatcag caaccggttc aaccatgatc tggctatgac ccgtttcatc gaagcggcgg
     5461 atcatctctg ccaggttatc ctgtgacaaa tcggattcat attcatccag aataacgtca
     5521 ggcaaaataa cagccacagg ttcatcaccc actaccgggt gagcacacaa taccgcgtgt
     5581 cccaggcctt tcgccagccc ctgacgaact tgcataatag ttacgtgcgg cggacaaata
     5641 gactgcactt catcaagcag ttgacgtttt acacgttttt ccagcattgc ttccagttca
     5701 aaactggtat caaagtggtt ttcaatagag tttttagatg agtgtgtaac cagcacaatt
     5761 tcagtaatgc cagccgcaat acattcattc acgacgtatt gaattaatgg cttatcgaca
     5821 agtggcagca tctctttcgg gatggctttc gtcgccggca acatcctggt tcctaatccc
     5881 gcaacgggga taacggcttt tttgactttc gtattaatgg cagccattta aattctcctg
     5941 gactgttcgt gttttgaacg tgttcatatt tctgtatcgc atcccagtat atcagcaccc
     6001 cggaggggct aagttctgaa aaggatgcga gttagcataa ttaagacaat tacttatacg
     6061 aatggcgacg atagtaccac cagccgacat tagctggtaa tgcaaattta gcccgcgtta
     6121 tcgtttgctc attctgcaga caacatcaag cgcagtcgac caccggttcc ccatatttgg
     6181 cattgccagg catcgcatcg ctggctcaat tgattcaaat aagcgttacc taaagtgcct
     6241 aacggaacac cattactgat ttgcacctgg tgttcgccgg tatttaacgt cgcattcaga
     6301 cccgcagaaa ccagaatgag atttttcagt tcgcgatgat aatagccaac taatagcgga
     6361 aactgccccg gcagattggc ctgacggagt aaatggttta cctgcttcaa taacgcgccc
     6421 aactctggca accgttgatt ttggtgtgca agctgttcct gtaataatcc gttaaacaat
     6481 gcgcgtaata acaaggcagc aagtacgcca ttatgtccag ctcgggttac atcaaggcaa
     6541 taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa gcaccaggcc gggtttgtcc
     6601 gcggcaacca attgacggta attaacccgg caatgggaaa tcacctgctg aaccggcggt
     6661 tgtagttcct gtaataattt cgccgctgcg gcagggttat caaccattgc atcccagtcg
     6721 cgaaaaagcc tttcctcttc ctcaacgcgc gaattaaaca tgctgggata gagacaggca
     6781 aaaaccatct cgcgcaagcg attcagatct ttaactggtt tcagcaaaac atcttcaacg
     6841 cccagacgta acgctttggc aatatctgcc atattttcag tggcagatat caccagaact
     6901 ggggtctggt cgcctctgtt acgtatatgc tccagcagtt taagcccgtt cattcgtggc
     6961 atcgcgatat cacatatcat caggtctgga gtgaaacctc ccagcaactc aagggcatcc
     7021 accccatcag ccgccagtac cgttgtcgct cccaatgagg aaaaccacga atccagaagc
     7081 gagcgaaata cctgctcatc ttcaacaatg agaatctgtt ttccgaccaa tggctgcgtc
     7141 atgttctctc ccctgactgg ctttactcaa tagtggcatg ctattgccac ctgcgcctgt
     7201 cagaattaac ttaagtgtag ataaaaattc tggtcaaatg ttggtgcgta ccaacggcaa
     7261 aagttcgtcc attttctttt ccactgccag ctgcccggcc gcaatggcag cgtgcgcgcg
     7321 atggaaatca agcgtagata tttgcgggca aacaggttga atcagaatat cgggcggatc
     7381 acctgccatg cggttccttt taaggcggtt ctccagcacc tggatagaag tggtcataat
     7441 ctctgttgcc gttggtgctg tcaccgcccg acgtgtcgtt atactgccta acctttcttt
     7501 cagacgcgca tgccacggca gagaatcacc attctcgcta ttttcttcac tgacattaaa
     7561 ggagagcaaa tcttgttgca tcaaatgagc atcgtgctgc aggtcgaccg ctatcacaat
     7621 atcagcaccc aatgcacgcg tgagcgaaat aggaattggg ttaacgactg ccccatcaac
     7681 cagccagtag ccgttatgcg caacgggtgc cattagtcct ggaatactgc atgatgcgcg
     7741 aatagcaaga tggagatcgc cttcagtaaa ccataattca cgcccagtac ttaaattggt
     7801 ggcaacagcc gcgaagcgac gggaacaatt ttcgatctct gtttccggca ttatttcgcg
     7861 gtattgattg aagacacgct cgccgcgcag taacccaccg cgctgccagg agagatccat
     7921 cagtcgtaaa acatcccaat aactgaaaga ggtcacccaa tcttccagcg cagataatcg
     7981 atcgcatgca taggccgcgc ccaccagcga accaattgaa catcctgcaa cgatatcaat
     8041 ttcaataccc acttttttta gcgcattaat aacgccaata tgcgaccaac ctctcgccgc
     8101 gccagatccc agcgccagcc ctatttttat ctttctcatt attcccgcaa gattcccctg
     8161 gaatgcaatc gtagccacat cgctactgct cggttaacat agtgctactc aggcggttaa
     8221 tacgccgtta ttgtttccag ggagattatt tgtgtctcag ctttgtccct gtggtagtgc
     8281 tgtcgagtat agcctatgtt gccaccctta tgtgtctggt gaaaaggttg cacctgatcc
     8341 agaacatctc atgcgttcgc gttactgcgc ttttgtgatg caagacgcag attatttaat
     8401 aaagacctgg catccatctt gtggagcagc ggcgttacgt gccgaattga ttgccggatt
     8461 tgcgcatact gaatggttgg gattgaccgt atttgaacat tgttggcagg acgccgacaa
     8521 tatcggattt gtcagttttg ttgctcgatt taccgaaggt ggcaaaacag gtgcaataat
     8581 tgaacgttcg cgatttttga aagaaaacgg tcaatggtac tatattgacg gtacacgtcc
     8641 gcagtttggc cgcaacgatc cctgcccttg tggttcaggt aaaaaattta aaaagtgctg
     8701 cggccaataa tggttgacgg tacggtttag caaacactct caacaaggtt tttccagcaa
     8761 tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc ggaccaaaaa ggtctgatcg
     8821 cacgtattac caatatttgc tacaagcacg agttaaatat cgtacagaac aatgaatttg
     8881 ttgatcaccg taccgggcgc ttttttatgc gcacggaact ggaagggatt tttaatgatt
     8941 ccaccctgct ggcggatctc gatagcgcat tgccagaagg ctccgtgcgt gagctgaatc
     9001 ctgccggtcg tcgccggata gtgattctgg tcactaaaga agcgcattgc cttggcgatt
     9061 tgttgatgaa agccaattat ggcggcctgg atgtcgaaat cgcggcagtg attggtaacc
     9121 acgatacttt acgttctctg gttgagcgtt ttgatatacc gtttgagctg gtaagccatg
     9181 aagggttaag ccgcaacgag cacgatcaaa agatggcgga tgccattgat gcttatcaac
     9241 ctgactacgt ggtgctggcg aagtatatgc gggtattaac accggaattt gtgtcacgct
     9301 tcccgaataa gatcatcaat attcaccatt ccttcctgcc agcgtttatc ggcgcacgtc
     9361 cttatcacca ggcctatgaa cgtggcgtga agattattgg cgcaaccgct cactatgtga
     9421 atgacaatct ggacgaaggc ccaatcatca tgcaggacgt tattcatgtc gatcatacct
     9481 acacagctga agatatgatg cgcgcaggtc gtgacgtcga gaaaaacgtc ttaagtcgcg
     9541 cgctctacaa agtactggcg cagcgcgtct ttgtttacgg taatcggacg attattcttt
     9601 aatcgccagc aaaaataact ggttaccttt aatccgttac ggatgaaaat tacgcaacca
     9661 gttcattttt ctcaacgtaa cactttacag cggcgcgtca tttgatatga tgcgccccgc
     9721 ttcccgataa gggagcaggc cagtaaaaag cattaccccg tggtggggtt cccgagcggc
     9781 caaagggagc agactgtaaa tctgccgtca tcgacttcga aggttcgaat ccttccccca
     9841 ccaccatgat tcaccacagc gatgtggtaa cttcggtgag attgtgaaag tttgcactca
     9901 tcggggaagg gtgagaacct tcgactaagg ttcgactcga gcgaaagcga gagaacgttt
     9961 tcacaggcaa cggcccgaag ggtgaggagc gaagcgacga ataatccttt ccccaccgcc
    10021 atctcaagca ttacctcaaa atttgaatta cccctggtgg ggttcccgag cggccaaagg
    10081 gagcagactg taaatctgcc gtcatcgact tcgaaggttc gaatccttcc cccaccacca
    10141 tcactttcaa aagtccctga actctcaagc gaatccgtaa ataaatattc tgcccaagcg
    10201 gagaaggatg agaagcttcg actaaggttc gactcgagcg ccagcgagag agcgttgccg
    10261 caggcaacga cctgaagggc gaagcgcgca gcgctgagta atccttcccc caccaccatc
    10321 actttcaaaa gtccctgaac tctcaaacga atccgtaatt aaatattctg cccaagcggg
    10381 gaaggatgag aagcttcgac caaggttcga cttgagcgcc agcgagagag cgttgccgca
    10441 ggcaacgacc cgaagggcga agcgcgcagc gctgagtaat ccttccccca ccaccatcgc
    10501 ttcctgaaat aatcaaaacc tgttactccg agctaatttt gcccaatggc accacgttta
    10561 ccggacctac aatatcctcc cttgtaggtc ggataagcga agcgcatccg acaactcacc
    10621 ccgcaccgtg cccataaaaa aaccccgccg aagcagggtc tatgtgaact aaaattcgct
    10681 tagtgacgag cgcgcaccag ctggtactta cgtgtcagat actccaccgg tacgctccag
    10741 atgtgtacca gacgcgagaa cgggaacagc aggaataacg tcatccccag caccaggtgt
    10801 agacggaaga taaacgccac accatcgagg tgttgagaag cgccaccgtg gaaggtcact
    10861 accgactgcg cccagccaac cagtttcatc atctcgctac cgtccatatg ttgggcggag
    10921 aacggaatgg tcaacaggcc cagcgcgcac tggataacga gcagcgacag gatcagtata
    10981 tccgctccgg tagtcgttgc acgcacgcgt gggctgaaca gacgacgttt aagcaaaagc
    11041 acgccgccaa tcagacacag cacgccgctg gcaccaccag caaacatcgc cattttctgt
    11101 ttcacttcaa tcggcagcca ggcttcatac atccagtgcg gcgtcaacat accgaagaag
    11161 tgaccgacaa aaatccccag aataccgata tggaacaggt tcgacgccag gttcatccct
    11221 ttgcgatcca gcatctggct ggacgccgcg cgccaggtgt actgcccgta gtcataacgc
    11281 agccagctac caatcaggaa gaccgccccg gctatgtacg ggtagatatc aaagaagaac
    11341 atattcagga attgcattag tgctgtcctc cggtggtgat attcagatat tgcggcgcga
    11401 cggcaccggc aaagcgacgc tgatgagcag tgattgctga atcaccgcag cctttgtcag
    11461 caaagaattt aacctgctct tcttcccaaa cagcatccag cgcctgcggc gtatcatcgc
    11521 gcgcttcgtc ggcaattttt tccgccactt tgtcgctgtc gatagcggta ttggccaatt
    11581 tcagcagcag atcaaacagc acggcataac ggctttcacg ctgttgcaga cgcgcactca
    11641 gcaatgccag aatcggcgcg atatctttca aaccttccac ggcttcgctt tgcggcagct
    11701 gcgccaggta ctccagatac agcggcagat gatccggcaa ttcgcggctg tttaactgca
    11761 agccgtgctg ctcgtactgc gccagcaggt ccaccatcgc ctggccgcgg tcgcgggatt
    11821 cgccgtgcac atgttcaaac agcaacagtg acgtggcgcg gccacggtcg aacagttcgc
    11881 tgtactgcgc ctgggcatcg agcggatcca tcgtcgttaa atcgcgcagg aaaatgccca
    11941 gcgcatgggc atcctctttt ggcagatttt tcgacgcggc aatcgcctca aacatctctt
    12001 gttgatgctg ccataaggca gcatccggat attcaaggag acgcgataca atcacgagtt
    12061 cgatcatgga tgcggctccg ttttgctggt cacatcgatg gcatcgatac gacggctgtt
    12121 gaacagattg aatttggtat ctgaaccgtg gcagccatca ccaaaggtaa agccgcagcc
    12181 atttttctcc gggaaggctt cccgtgccag ttcacgatga ctactcggca ccacaaagcg
    12241 atcttcgtag ttagcaatcg ccagataacg gtacatctcc tgtgcctggg cttcggtcag
    12301 accgacctct tccagcgcac gggtatcaac tttaccgtca acggtttcag cacgtttgta
    12361 atgacgcatc gccagcatac gtttcagcgc gcgcagtacc ggtttggtat caccggcggt
    12421 cagcagattc gccagatact gtaccggaat acgcaggctt tcgacgtctg gcagaatgcc
    12481 gttgctaccc agctcacccg cgtctgctgc agactgaatc ggagagagag gcggcacgta
    12541 ccagaccatc ggcagtgtgc gatattctgg atgcagcggc agcgccagtt tccattccat
    12601 tgccatttta taaaccggcg actgctgtgc agcttcaata acgctcagcg gaataccgtc
    12661 tttaatcgcc tgctcgatga ctttcggatc gttcggatcg aggaacacct ccagctgacg
    12721 ctggtagaga tctttctcgt tctcggtgct ggctgcacgt tcaatcgcgt cggcatcgta
    12781 cagcagcacg ccaagataac ggatacgacc gacacaggtt tctgagcaca cggtcggctg
    12841 acccgcttca atacgcggat agcagaagat gcacttctca gacttaccgc tcttccagtt
    12901 gaagtagatt tttttgtacg ggcatccggt gatgcacata cgccagccac ggcatttatc
    12961 ctggtcgatc aggacgatgc catcttcttc acgcttgtaa atcgcaccgc tcgggcaggt
    13021 cgccacacat gccgggttca ggcagtgttc gcacaggcgc ggcaaataca tcatgaaggt
    13081 gttttcgaac tggctataca tcgccttctg gatgttgtcg aagttcttgt ctttcgccag
    13141 tttgtcaaac tcaccgccca ggtcatcttc ccagttcggc cctttttcga ttttcgccat
    13201 ccgttccccg gtaatcagcg aacgcggacg ggcaatcggc tgcgatttgc tgccttccgg
    13261 cgcggtatgc aggttctgat agtcaaaatc gaacggctcg taataatcgt cgatccccgg
    13321 cagatgcggg ttagcgaaga ttttacccag cagcatggca cggttaccca tgcgcggttg
    13381 cagtttgccg ttgattttac ggatccagcc gcctttgtat ttttcctggt tttcccagtc
    13441 agtcgggaag ccctggcccg gcttggtttc cacgttgttg aaccacgcgt attccacgcc
    13501 ttcacggctg gtccagacgt ttttacaggt aactgaacag gtgtggcagc cgatgcactt
    13561 atcgagattc agcaccatgc cgacttgtga acgaattttc attttacgct ctcctgtacc
    13621 tgatcattgc cttcgccatc taaccagtca atgttcttca tcttacgcac tacaacaaac
    13681 tcatcgcggt tagaacctac ggtgccgtag tagttaaagc cgtatgccag atgggcatag
    13741 ccgccgatca tatgcgtcgg tttcggcgtg atacgggtga ccgagttatg gataccacca
    13801 cgctgttggg taatttccga accaggcagg ttaacgatac gttcctgcgc gtggtacatc
    13861 atggtcatcc ctgccggaac acgctggctg acaaccgcac gggcagtcag agcaccgttg
    13921 ctgttgaata cttcaatcca gtcgttatcg gcgataccca gatctttggc atcggcttca
    13981 ctcaaccaga ccaccggacc accgcgacct aaagtcagca tcagcaggtt gtcgctgtag
    14041 gtggagtgga taccccactt ctggtgcggc gtcaggaagt tgagcgcttt ttccgggttg
    14101 ccgttggatt tctggcctat cacttctttc accgaacggg tgtcgatcgg cggacgataa
    14161 accagcaggc tttcaccgaa atcacgcatc cactggtgat cctgatacag ttgctgacga
    14221 ccagagagcg tacgccatgg gatcagctcg tgaacgttgg tgtaaccggc gttgtaagaa
    14281 acgtgttcat cttccagacc agaccaggtc gggctggaga taattttgcg cggctgtgcc
    14341 tgaatatcgc ggaagcggat cttctcgtct tctttattca gcgccagatg cgtatggtca
    14401 cgaccggtaa attcgctcag ggcagcccag gctttcacgg ctacctgacc gttggtttcc
    14461 ggtgccagtg tcaggatcat ctctgccgca tcaattgcgg tgttcagcat cggctggcct
    14521 ttcgccggac cttccgcttt ggtgtagttg agcttacgca gcagatccat ctcgctctgg
    14581 gtgttccagg caatcccttt accgccgtta ccgattttct ccatcagcgg gccgatagag
    14641 gtaaagcgtt cgtaagttgc cggataatcg cgctctacga ccataatgtg cggtgcagtt
    14701 ttgcctggga tcaggtcgca ttcgcctttt ttccagtctt tcacatccag cggctgcgcc
    14761 agttcagcgg cagagtcgtg ctggataggc agcgtgacga tgtcggtttc tttacccaga
    14821 tggccgacgc acacttcgga gaatttcttc gcgatggctt tgtagatttc ccagtcgctt
    14881 ttcgcttccc aggccggatc gaccgccgca gacagcgggt gaataaacgg atgcatatcc
    14941 gaagtattca tgtcgtcttt ttcgtaccag gtcgccgtcg gcaaaatgat gtcggaatag
    15001 agacaggtgc tcgacagacg gaagtccagc gtaaccacca gatccagctt gccttccaga
    15061 ccattgtcct gccagtccac ttcttccggc ttcacgccgc cctgctgccc cagatcttta
    15121 ccctggatac cgtgctccgt ccccagcagg tacttgagca taaactcatg acctttaccg
    15181 gaagaaccga gcaggttaga acgccagata aacaggttac gcgggtggtt tttgccgttt
    15241 tccggctgtt ccgcagcgaa acggatagaa ccctctttca gggatttcac cgtatagtcc
    15301 accggattca tcccggcttt ttctgcttcg ccagcgatag tcagcgggtt agtgcctaac
    15361 tgcggcgcag acggcagcca gcccatgcgt tctgcacgga cgttaaagtc gatcagatgt
    15421 ccggtatagc gggatttatc cgccatcggc gacagcagtt cttccgcagt cacggtttca
    15481 taacgccact ggctggagtg gttatagaaa taagaggtgc tgttcatgtg acgcgccgga
    15541 cgctgccagt cgagggcaaa cgccagcggc tgccagccgg tttgcggacg cagtttttcc
    15601 tgacctacat agtgcgccca gccgcccccg ctctgaccga cacagccgca gaaaatcagc
    15661 atgttgatca gaccacgata gttcatatcg aggtgatacc agtggttcag ccccgcaccg
    15721 acgataatca tcgaacgacc gtgcgtttta tcagcgttat cggcaaattc acgggcgatg
    15781 cgaataatct ggctgcgaga aacgccggta atctgctcgg cccaggccgg ggtataagct
    15841 ttcacatcgt catagctggt tgcacagtta acgtcgttca ggccacgttc cagaccgtag
    15901 tttgccagcg tcagatcata aacggtggtc accagggcgg tgctgccatc agccagttgc
    15961 aggcgtttca ccggcagttt gtgcagcagc acgttttcca gttccacttt gttgaagtgt
    16021 tccgtgccgt cgccaccaaa gtacgggaag cctacctcag cgatctcatc ctggctaccc
    16081 agcaggctca gttgcagctc ggtttcttcg ccagttttgc cgtcgcgctg ctcaagattc
    16141 catttgccct tctcgcccca gcggaagcca atagaaccgt tcggcgcaac catttcgcca
    16201 ttggtattaa aggcgacagt tttccattcc ggattgtttt cctggcccag cgcatcaacc
    16261 agatcagcag cgcgcagcat acgacctgca gcgtagtaac cgtcgcgttc ttccagcatc
    16321 accagcatcg gcatgtcggt gtagcgacgc acatagtcgg tgaaatactg gcttgggttg
    16381 tcgagatgga attcacgcag cattacgtgg cccatcgcca gcgccattgc cgcatcggtg
    16441 ccctgtttcg gtgccagcca cagatcgcac agtttggcga tttcagcgta gtctggtgtg
    16501 acggcaacag ttttggtgcc tttgtaacgc acttcagtaa agaagtgagc atctggggta
    16561 cgcgtctgcg gcacgtttga accccaggcg atgatgtagc tggagttgta ccagtcagca
    16621 gattccggta cgtcagtttg ctcgccccag gtttgcggag acgcaggagg caggtcgcag
    16681 taccagtcgt agaagcttaa gcaagtaccg ccaatcagcg agagatagcg tgcacccgat
    16741 gcgtaagaaa ccatcgacat tgccggaatt ggcgagaaac cagcaacacg gtccgggccg
    16801 tagtttttga tggtgtaaac gttagatgcg gcgatcagtt cgttcacctc ctgccaggaa
    16861 gaacgaacaa aaccaccgcg tccacgcgcc tgcttaaagc ttttcgcttt atcggcgtct
    16921 tcaatgatag aagcccatgc ctcaaccgga tcgctatgca gcgccttcgc ttcacgccac
    16981 attttcatca ggcgtttgcg catcatcggg tatttcaggc ggttggcact gtaaagatac
    17041 caggagtagc tggcaccgcg cgggcagccg cgaggttcat ggtttggcag atccggacgg
    17101 gtacgcggat agtcagtctg ctgggtttcc caggtgacca gaccgttttt gacgtagatt
    17161 ttccagctgc aggagccggt gcagtttacc ccgtgggtag agcggacgat tttgtcatgc
    17221 tgccaacgct ggcgatatcc atcctcccag tcacggttgg tattgagaag ctggccatgc
    17281 ccatcggcaa aggtttcacc cttctgtttg aagtagcgaa accggtccag gaatttactc
    17341 atcggttttc tcctgtggga gcctgtcggc cctctgataa atcgacattg ctaatgttta
    17401 agggcaaggt aacgctctga aacagcgtgg gaattgataa cgatcaagag tgatggggat
    17461 gaaaaataaa gtaaattcct aagatacccc tttaggataa ttaacagtta cagcataaca
    17521 tgctgtaaat taagacgttt tatacctgta gttatttaaa ctattaagat ttttatgcaa
    17581 atgggtatta aggagtattc cccatgggta acatattaat ggttcttaat gaaatccggc
    17641 gaaataaacc atataacatt agtatgttgt aactaaatga cgcataaaaa aagcgcgaca
    17701 tcatgccgcg caaaggataa tcaaatatta agatttattt tttagaatgc cgaccatata
    17761 ccgcccaggt aatcactacg caggcgatat agaaaatcaa aaatactttc attgcgccga
    17821 ctggcgaacc cgttaatgcc agcgagctac cgaacgcttt cgggataaag aagccaccaa
    17881 tcgcgccaat cgcagagatg aaacccaacg ccgccgccgt gtcggttgcc gcttcacgca
    17941 tcgcacgttc gtcagaaccc ccttctgctt tcacgcgatc cattgtcagt ttacggaaga
    18001 tcacggaaat catctggaaa gtggaaccac tacccagccc agctgtcagg aacagcgcca
    18061 ggaagaccgc gaagaacgcc atgaagcttc cgccctgccc gtcagtcggt aaggtcagga
    18121 acagcaggcc gctgaaaatc gccatcagaa taaagttcac cagcgtgaca cgagttccgc
    18181 ccagacggtc agataatgca ccacctgcag aacgcgccag cgcaccaata aacggcccga
    18241 agaaagcgta ttgcagaatc tgaacatccg ggaactgcgt ttttgacagc atcgcaaagc
    18301 ccgcggagaa gccgatgaag gaaccgaagg ttgccagata cagcaggctc ataatccaca
    18361 gatgaccccg tttgagtacc ggcaactgct ccttgatgga ggctttcgag gtagcaagat
    18421 cgttcatgcc aaaccacgcc gcaatggtga agatggcaag gaacggcacc catatccagg
    18481 acgcattcgc cagatacagc tcagtcccat ccggctgttt gacgccctgg ctaccaaata
    18541 ctgcgaaaat cgacagtgat accaccagcg gagcaaccaa ctgcataacg ctaacgccca
    18601 tgttgcccag accaccattc aggcccagcg cgccaccctg cttctgtttc ggaaagaaga
    18661 agctgatgtt tgccatactg gatgcgaagt tcgcgccagc aaagccgcac agcagagaga
    18721 tgatgatgaa gacgctataa ggcgtggagg tatcctgcac ggcaaaaccc agccagacac
    18781 aaggaataat cagaataccg gtgctgaacg ccgtccagcg acgaccaccg aagataggaa
    18841 ccataaagga gtatggaaca cgtaataacg cgccagaaac cgaaggcaac gcagtcaaca
    18901 taaatagctg atcggtcgta aaattaaaac cgactttcgg taagttcacc gcaacagcgc
    18961 tgaacaacat ccatacgcaa aacgccagca gcagacaggg aacggaaatc cacaggttgc
    19021 ggctggcaat acgttgaccg cgttgttgcc agaacgcagg atcttccggt cgccaatctg
    19081 taatgacagc tccagtagcc ctttcggggg cggatgagtg actcatagac acctctgata
    19141 ctcgtttcga tgtctgccac cttagtgtct gtagctaaag gcaatttgat gtaaatcaaa
    19201 cgataagata actttatcta tcattgatat ttatcattac ccatagtgag tacagtgatt
    19261 tcataaaaat tatgagattt ttcacggtgc tgtaaaatcc ctacccttac cgatgtaaag
    19321 ctactaacca cacggcaaat aaggagtaac tctttccggg tatgggtata cttcagccaa
    19381 tagccgagaa tactgccatt ccagaatgta tcgtcacatt cattaaggtt attgctcatt
    19441 taaagcctga aggaagaggt ttacatgctt aaacgttgtc tctctccgct caccctggtt
    19501 aatcaggttg cgcttattgt gttgctttct actgctattg gactggcagg gatggcggtt
    19561 tctggctggc tggtgcaagg cgttcagggc agcgcccatg cgatcaacaa agcgggatcg
    19621 ctgcgcatgc aaagttaccg tctgttggca gcagtgccat taagcgagaa agacaagccc
    19681 ttaattaaag agatggaaca aacggcattt agcgccgagt tgactcgagc agcagaacga
    19741 gacggacaac tggcgcaatt acagggttta caagattact ggcgtaatga actcatccct
    19801 gcactgatgc gtgcacaaaa ccgcgaaacg gtgtcagcgg atgtcagcca gtttgttgcc
    19861 gggcttgatc agctggtatc tggttttgac cgcaccacgg aaatgcgcat tgagacagtg
    19921 gtactggtcc atcgggtaat ggcggtattt atggcacttt tactggtgtt cactattatc
    19981 tggttgcggg cgcgactgct acaaccgtgg cggcaactgc tggcaatggc gagtgccgtc
    20041 agccatcgcg attttaccca acgcgcaaac atcagcgggc gcaacgaaat ggcgatgctt
    20101 ggaactgcgt tgaacaatat gtctgcagaa ctggccgaaa gttatgccgt acttgagcag
    20161 cgggttcagg agaaaaccgc cgggctggag cataaaaatc agatcctctc ttttttatgg
    20221 caggctaacc gccgtttgca ttcccgcgcc ccgctgtgtg aacgcctgtc acctgtactc
    20281 aacggcttac agaatttaac cctgctacgt gatatcgaac tgcgggtgta tgacactgat
    20341 gatgaagaga atcatcagga gtttacctgc cagccagata tgacttgtga tgataaaggc
    20401 tgccagctct gcccgcgcgg cgtattaccc gttggtgatc gcggcacgac cctgaagtgg
    20461 cggctggctg actctcatac gcagtacggt attttgctgg cgaccctacc gcaggggcgt
    20521 catcttagcc atgatcaaca acaactggtg gataccctgg ttgaacaact caccgccacg
    20581 ctggcgctgg atcggcatca ggaacgtcag caacagttga tcgtgatgga agagcgtgcc
    20641 accattgcgc gcgaactgca tgattctatt gcccaatctc tctcttgcat gaagatgcag
    20701 gtgagttgtt tacagatgca gggcgatgcg ctgccagaaa gcagccgcga actgttaagt
    20761 cagatccgta acgaactgaa tgcatcctgg gcgcagttgc gtgaattgct caccacattc
    20821 cgcttgcagc tcaccgagcc tggattacgt ccggcgctgg aggcgagttg cgaagagtac
    20881 agcgccaaat ttggcttccc ggtgaagctg gattatcaat tgccgcctcg tctggtgcct
    20941 tcgcatcagg caatccactt gttgcaaatt gcccgtgagg cattaagtaa cgccctcaaa
    21001 cattcgcaag cgagtgaggt cgtggtgacg gtggcgcaaa acgataatca ggtcaaactg
    21061 accgtccagg ataacggctg cggcgtgcct gaaaatgcca tccgcagcaa tcactacggc
    21121 atgataataa tgcgcgaccg tgcgcaaagt ttacgaggcg attgccgcgt ccgccgtcgt
    21181 gaatcaggtg gcaccgaagt ggtggtcacc tttattcccg aaaaaacttt cacagacgtc
    21241 caaggagata cccatgagta atcaggaacc ggctactatc ctgctgattg acgatcaccc
    21301 gatgctgcga actggcgtaa aacagcttat cagtatggca ccagatatca ccgtggttgg
    21361 cgaagcgagt aatggcgaac agggtattga actggcggaa tctcttgatc ccgatctgat
    21421 cctgttagat ctcaatatgc ccggcatgaa cggtctggaa acgctggata aactgcgcga
    21481 aaagtccctc tcagggcgca ttgtggtatt cagcgtctct aaccatgaag aagatgtggt
    21541 caccgcactg aaacgcggcg cggatggcta tctgttaaaa gatatggaac cggaagatct
    21601 gctgaaagca ttgcatcagg cagctgctgg cgaaatggta ttaagcgaag cattaacgcc
    21661 tgttctggcc gccagcttgc gcgctaaccg tgccacgact gagcgcgatg ttaaccagtt
    21721 aaccccacgc gagcgcgata ttctcaagct gattgcccag ggtttgccga acaagatgat
    21781 tgcccgccgc ctggacatca ccgaaagcac agtaaaagtg cacgtcaagc acatgctgaa
    21841 gaaaatgaag ctcaagtctc gcgtggaagc agcggtatgg gtgcatcagg agcgcatttt
    21901 ctgattacgg ttcccagcgc agttcgtcgt ttgacaatgc gtcgaacggt tcgacaagcg
    21961 ttagcgtgat ctcattggag gagacacgct gcccctggtt atcttccacc accaccgaca
    22021 atcgccagtg attgctcgcc ccttccccgt tctgccagtc aggcatgatc agcgtccagc
    22081 cctccgcgct gttggcttgt gcgcccggcg tcaaacttaa tatctgcgta tcgccctgcc
    22141 aaatcagttg ccgaataccg taacgactgc ggatttgtaa tttcagcggc actgtttcgc
    22201 caggttttag atcccacggc ggtgtcgcaa gaaacaccgt taacgttttt cgctgtcggt
    22261 actcaagagt cggtagatta tttcgctgcg gattatcata gcgactacca cgtaacgact
    22321 gactttcggc aacctcgccc gcagaaagtt gttttttgag cggtacacca aagcggtaat
    22381 taagattcag cccgaggtta ttttgattct cgccactttc accctgttta tgctgggccg
    22441 tcacagtgac taatggcaca ggggtgtaat ttaatcccag actcaacgcg acgggattgt
    22501 gataacccgt accagagtta aacaaatcaa cacgatcacc aaaatactgt tctacgctga
    22561 cactggtatt gaggtgttga tagaacggca tgcgcatccg ggctgtcagg tcgtacccgc
    22621 gcgccatccg ttgttcctgc gtggctgtct gttcatgcca tgcagcaaac ggctgataaa
    22681 agtttgccga tagtcgcaaa tattcgcccc acgcttcggc accaaagccc gctcgctgaa
    22741 gattttcatc cagcaagttg tcataaaaag tgttataacc caccagccag ttgccgcgcg
    22801 cccagcgttg cccaacgccc acattgctca ccagcccatc atcctgctga gtaagaccaa
    22861 gctggctcca ggtgagataa cgatcattat cttgtaacgg cacaaaccag cttccacgac
    22921 tgccggtaaa atgaccttcg ttatccactt tgacgtcaac actggcattt ccccacggtg
    22981 atagccacga ctctacgtgc tgattaacct gttgactaag cgcgtcgcgg acttttccca
    23041 atgcgaaagc ttttgcctgc tcgccagtat ccagcccgtt atcattcata ctggtttcgc
    23101 cgaaatcttt cacaatttca gcaaagtgtt tttccccatc atgattttca ggcgccatgc
    23161 ctaaatccgg cagaccatca ttgttattat caaagggatt tgccgctttt tgctcgaagg
    23221 tagattgtgc gttagctgta ccgcctgccg ccagcaagag taaataaaac ggaataatgc
    23281 gaggaacgaa acggctcaaa acgtcggtaa cttccggtaa aaaatgaaaa aacatagcct
    23341 taaagaataa cgattactgg caacttttgt cgtattttct cgcgctttca ggaaaatcct
    23401 taaaaatgtg accgattggt cagacgctgc agaagcagta agatagaaga tgagtgtcta
    23461 attccctgaa tacaggagta aataatgcaa aaaatcgtga tcgttgccaa tggcgcacct
    23521 tacgggagcg aatccttgtt taacagcttg cggctggcca ttgcgttacg agagcaggag
    23581 agcaatctgg atctgcgtct gttcctgatg tctgatgcgg tcacagccgg gttgcgcggg
    23641 caaaaaccag gggaaggcta caacatacag caaatgctgg agatccttac cgctcagaat
    23701 gtaccggtga aattgtgcaa aacctgtacc gacgggcgcg ggattagtac acttcctctg
    23761 attgatgggg tggaaatcgg tactcttgtg gaactggcgc aatggacgct gtcagccgat
    23821 aaagtgctca cattttaatg tttgaggggg cgtctgacgc cccacgatga gggaagttta
    23881 ctcaggcgaa tcccggccgc aacacaccat ccggataatt ctccgccagc agttttttta
    23941 ccgataccag caaatcattc agcccatcgt cctgcattcc cagtttgata agctcctgtt
    24001 ccagtgaaaa caggtattgt gcgttggttc ccagcggacc gctcgccgcc gcaatcaacg
    24061 gcgcgatgac ctgagcgcga gtgtcagatt catattctgg atgtcgcggg tccataataa
    24121 acacaatggc gtttactgtg cgtccatcat caagatcaag ctgacaccag gttggcagat
    24181 aacagccggt aatcatctcg cgcttccaca acagggttag ttcctgctcc agcgtctctt
    24241 ctggcagtcg ataggcgacg cctgtggtac gtccgccctc ttttagtgca agcatccgtc
    24301 ccggctggtg cgcagtcccg cgcccggcgg tcaggcgcag gcaaaacgcg cgatgccatc
    24361 caaccagtgt accggtgcac gactcggtaa actccagtgc cggattccac atcaacgatc
    24421 cataaccgaa gatccacacc ggtctctcat caggtcgaca agccagcgtc gccgccagcg
    24481 aagccgcccg ttgttctgct gaccataaga gtgattcttc aatggcacca aatgccgttt
    24541 tacaatcggc attcatcaag aaatcacgcg ttatcacctt cttacacctc cgccactgca
    24601 tgcttccctg cgacaacttt ttggtgtctt ccggacattt ttactgctca ttttgaaacc
    24661 actaacggga caatatgcaa tccgcggacc acttgcaagg caacaccgca cgaatagctt
    24721 taactaagct accggtttta cgatttttta tgccatttat catcatcccc tttggcatat
    24781 tcatgcttca cagcagccca ggccacttta tgcgcggttt cttcgcgact ggcgtcatca
    24841 cgccgatctt ctttatcttt atattgatcc catgcgctgt tgaacgcttc tttatagata
    24901 tcctgggcat gagacggtag aacgtgcttt acgctttccg gcaggtcgct tttcgtttta
    24961 tacggcatcg ttgactcctt ttacaaagtt gaacattaag tgtggtcaac aatatctact
    25021 cttgcaaaaa cataagaatt ttcatgtcag actgttctta agaaacgcta aaacaaacat
    25081 tgcctaaacg taatacttat ttttatgccc caatgagtaa aaaa
//
LOCUS       DAZFDC010000065        25081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFDC010000065 DAZFDC010000000
VERSION     DAZFDC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.065"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..103)
                     /locus_tag="ACHJ48_002729"
     CDS             complement(<1..103)
                     /locus_tag="ACHJ48_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708255.1"
                     /translation="MTISNSNLLKPFITGTELPPFKEDVFSKTEEQFK"
     gene            complement(291..2108)
                     /gene="malZ"
                     /locus_tag="ACHJ48_002730"
     CDS             complement(291..2108)
                     /gene="malZ"
                     /locus_tag="ACHJ48_002730"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO4708256.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGREVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     MALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(2264..3637)
                     /gene="proY"
                     /locus_tag="ACHJ48_002731"
     CDS             complement(2264..3637)
                     /gene="proY"
                     /locus_tag="ACHJ48_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO4708257.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(3713..5032)
                     /gene="brnQ"
                     /locus_tag="ACHJ48_002732"
     CDS             complement(3713..5032)
                     /gene="brnQ"
                     /locus_tag="ACHJ48_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO4708258.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(5439..6734)
                     /gene="phoR"
                     /locus_tag="ACHJ48_002733"
     CDS             complement(5439..6734)
                     /gene="phoR"
                     /locus_tag="ACHJ48_002733"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO4708259.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSVISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIVKNSD"
     gene            complement(6792..7481)
                     /gene="phoB"
                     /locus_tag="ACHJ48_002734"
     CDS             complement(6792..7481)
                     /gene="phoB"
                     /locus_tag="ACHJ48_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO4708260.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            7671..8873
                     /gene="sbcD"
                     /locus_tag="ACHJ48_002735"
     CDS             7671..8873
                     /gene="sbcD"
                     /locus_tag="ACHJ48_002735"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO4708261.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8870..12013
                     /gene="sbcC"
                     /locus_tag="ACHJ48_002736"
     CDS             8870..12013
                     /gene="sbcC"
                     /locus_tag="ACHJ48_002736"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO4708262.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQNTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLMLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            12139..13323
                     /gene="araJ"
                     /locus_tag="ACHJ48_002737"
     CDS             12139..13323
                     /gene="araJ"
                     /locus_tag="ACHJ48_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO4708263.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(13466..14374)
                     /gene="mak"
                     /locus_tag="ACHJ48_002738"
     CDS             complement(13466..14374)
                     /gene="mak"
                     /locus_tag="ACHJ48_002738"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO4708264.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            14499..15410
                     /gene="rdgC"
                     /locus_tag="ACHJ48_002739"
     CDS             14499..15410
                     /gene="rdgC"
                     /locus_tag="ACHJ48_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO4708265.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLTSMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMANPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(15495..15944)
                     /locus_tag="ACHJ48_002740"
     CDS             complement(15495..15944)
                     /locus_tag="ACHJ48_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708266.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKNYCPP"
     gene            complement(15941..18220)
                     /locus_tag="ACHJ48_002741"
     CDS             complement(15941..18220)
                     /locus_tag="ACHJ48_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO4708267.1"
                     /translation="MNRINILVICMVLFFMTGNACATEWISSEDLITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNKNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHWLTDELIKDEAVARGLVKNSNISADV
                     NKKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVAD
                     ELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKVLLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTESQDRSLAINNPQLAADVKTVWLKEDPSLLLFVDKPDLSQLRDLVK
                     TGATRKIRNEARHRLEEKQ"
     gene            complement(18364..18648)
                     /gene="ppnP"
                     /locus_tag="ACHJ48_002742"
     CDS             complement(18364..18648)
                     /gene="ppnP"
                     /locus_tag="ACHJ48_002742"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO4708268.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(18720..19397)
                     /gene="aroM"
                     /locus_tag="ACHJ48_002743"
     CDS             complement(18720..19397)
                     /gene="aroM"
                     /locus_tag="ACHJ48_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO4708269.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(19655..19846)
                     /gene="yaiA"
                     /locus_tag="ACHJ48_002744"
     CDS             complement(19655..19846)
                     /gene="yaiA"
                     /locus_tag="ACHJ48_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO4708270.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(19896..20420)
                     /gene="aroL"
                     /locus_tag="ACHJ48_002745"
     CDS             complement(19896..20420)
                     /gene="aroL"
                     /locus_tag="ACHJ48_002745"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO4708271.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(20603..21061)
                     /gene="yaiI"
                     /locus_tag="ACHJ48_002746"
     CDS             complement(20603..21061)
                     /gene="yaiI"
                     /locus_tag="ACHJ48_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO4708272.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            21181..21990
                     /gene="proC"
                     /locus_tag="ACHJ48_002747"
     CDS             21181..21990
                     /gene="proC"
                     /locus_tag="ACHJ48_002747"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO4708273.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDKFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(22007..23122)
                     /gene="adrA"
                     /locus_tag="ACHJ48_002748"
                     /gene_synonym="dgcC"
     CDS             complement(22007..23122)
                     /gene="adrA"
                     /locus_tag="ACHJ48_002748"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO4708274.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     NNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVNLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(23224..23544)
                     /gene="psiF"
                     /locus_tag="ACHJ48_002749"
     CDS             complement(23224..23544)
                     /gene="psiF"
                     /locus_tag="ACHJ48_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO4708275.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(23663..25078)
                     /gene="phoA"
                     /locus_tag="ACHJ48_002750"
                     /pseudo
     CDS             complement(23663..25078)
                     /gene="phoA"
                     /locus_tag="ACHJ48_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
BASE COUNT         6306 a         6666 c         6145 g         5964 t
ORIGIN      
        1 ttttaaattg ttcttctgtt ttagaaaaga catcctcttt aaaaggtggc aactcagtac
       61 cagtaataaa cggtttcaat aaattcgaat ttgaaattgt catatctttg ctccactaca
      121 gggttatcct ggagcatgtt gtacataaca tcatgggcaa acaataccaa aaatcgctca
      181 tatcgatcgg gacaaatatt taacaaattc agccctgaca ttgagcagct tttttgcgct
      241 gcgtagtgtc agggctggtg actaacgacg taacaaataa aacagatgct ttagttcatc
      301 cacaccgtag ccgaaatggc aggcaaagcc agaatcccgt ctgtcagttg cccatggcct
      361 tctttgcgtt gccattgcac gacattaaga aacggtgacg cgggtagcac cacttcacag
      421 gcttcgccac ggttaattgc caccagtaca cgctgctgat tcagcacgcg gacaaatacc
      481 accacgttat cttccgcata cagcacctga cagccgccac gacgcagcgc ctgacttttc
      541 ttacgcagcg caatcattcg ctggtacaac gcgaataacg ccatatcctg tttttccacc
      601 tgccagggga acggcttacg gcaaaacgga tcatttttgc catccagtcc cacttcatca
      661 ccgtaataaa tgcacggtac accaggccag gtgaacagcc agaccactgc caggggcagg
      721 cgcgcaacct cccgaccgag cagcgtttta aatcgcgcag tatcgtggct gtcgagctga
      781 ttaaacatac gtaattgctg ttgatgagaa agccctgcgc ggtaattatc catccaggcc
      841 atacaggttt gagcgtcaat ttgctgcgga tcgtaagaga tgtcggtatt ggcaagaaat
      901 ccccacaacg ggaatgtgaa gccgcgatag ttcatggcgg catcttccac atcggcctgt
      961 aaccattgcc gtgcatcgcc aaaatgctct ccaactatat acgcttccgg ctgggtttct
     1021 ttcgccgctt cggtgatccc agcaacgtgc tgcaaattat tgcgcgcccc acccgcttcc
     1081 cccagcatat gcaccacatc cagccgccag ccgtccatat tccacggcgc tttcagccag
     1141 tggcggacaa tactgtcttc cccgcgataa atttcattca ccagactttc cgactgataa
     1201 tccagcttcg gcaagctggc atagccaagc cagtcgagcg ccgtgccatc atcactaaac
     1261 gagtaccagt cgcgccaggg cgattcgggg ttgtgacaag ctccccccgt gccacgattg
     1321 tgcctgtcaa accaggcatg ggaatcgcca ctgtggttaa acacgccatc cagcaccagc
     1381 cgcattccca gctgctgcgt attgtggcgt aagcgcagca acgccccatc accgccaaac
     1441 tgcggatcga catggcgata atcctcggta tcgtatttat gtacgctggg agctttaaac
     1501 accggattga gatacagcgc cgtcacgcca agctttttca gatacggcag tttttcgctt
     1561 atcccgtcca gatcgccgcc atagaacgtt gatccgcccg cctgcgccgt gaccggttca
     1621 tcccagtcac gcaagatgat ctcttgtccg gctgcatgat ggtaatagac atgatcctgt
     1681 tcagcttcac gaggaagact acgcgcaaaa cgatcaggga agatctgata aaaaatctga
     1741 tccgcagccc attgtgggcc gatatccggt acatcgacgg caaactgctc cagtcgtgcc
     1801 ggcggcattc ggctgaagcc ctgcggtgta aaccaacgct ggcgatcgtg ccacagcagt
     1861 ttgaaactgt aacgccgccg gggttgtccg ctggagagat caatcgccgc acgccatgcg
     1921 gtgacgcctg gctgcggctg actgcgctgc ttatgcattg gtactgacat ttcttcgtta
     1981 tcgtgttctg tacgcagcat aatgcgctgc ggtgggtctt cgcccgtcag ccacagtgta
     2041 atgagcagtt gatctttgct ttgtttaaca aatgggggca ccggcaggtg ccatgcattt
     2101 aacatcataa ttcccctgtg atgaaaatct gatcaccttg ccatagccta atgcaggatc
     2161 actcatcatc tgaaggatat ttgggggagg aggacggggg cggataactt tcggaatagc
     2221 cggatcagga tttcctcctg aaccggctta cagaacgcaa gtattattgg ttttcagcca
     2281 gctgacgatc gtggcgacgc ttaaacatcc agccaatcaa cagcacaaca atccacgcga
     2341 aaccgacata cagcgagata cgcgtatccg ggtgataacc aatcaacccg ataataaaga
     2401 gcaggaaaat cagcccgccg atggtcgttg ctaccccacc cggcacttta aatttcagcg
     2461 ccttaacttc ttctggcggc aaacggcgac ggaaggcaat ttgcgacagc aggatcataa
     2521 tccacaccca caccgtggcg aaggttgcca gcgaagcgat caccaggaag acgttttccg
     2581 gcatgatgta gttcagatac accgcaaaca gcagcgcggt agtcatcacc agcaccgtaa
     2641 cccacggaat accgcgacgc gacgttttgc tgaaaatttt cggcgcgctg ccctgctctg
     2701 ccataccgtg gagcatacgg cctacgccaa atacatcact gttaattgcc gacagcgaag
     2761 cagtcagcac aacaaagtta agaatgctgg cggcaaaggt aatgcccata tgctggaacg
     2821 tcagcacgaa cgggctaccg gcagtgccaa cctgattcca cgggtagata gacataatga
     2881 cgaacagcgt accgacgtag aacaccagaa tacgcatcgg cacggagtta atcgcacgcg
     2941 gtatcgattt ctcaggatct ttcgcttcac cggcggtaat cccgataatt tcgatcccac
     3001 cgtaagcaaa catcaccatt tgcaacgaca ttaccatgcc aagccagccg ttactgaaga
     3061 agccgccgtt gctccacagg ttatgaatac cggtcggttg cccgccgttg ccaatccccc
     3121 agatgatgat gccgaaaccg gcgacaatca tgatgatgat ggtggcgact ttaaagaacg
     3181 agaaccagaa ttccagctca ccgaatacct tcacgctcat caggtttacg gcgcaaatga
     3241 tcagcaccac gctcagtacc caaatccagt gcggcaccgt cgggaaccag acacccatat
     3301 agataccaaa agcggtcaca tcggcgatgg cgacaataag gatttcaaag cagtaggtcc
     3361 agccggtaat gtaacctgcc agcgggccga ggttttcctg cgcataacgc gagaaagagc
     3421 tggcggccgg gttatgtacc gacatttccc ccagcgcacg cataatgata tacgccgcga
     3481 taccaccgat aatataggcc aacaacacgc tcggaccggc cattttgatg gcgtctgccg
     3541 aaccgtaaaa cagcccggtg ccaattgctg aacccagtgc cataaagcgt atgtggcggg
     3601 tacttagccc acgctttagc ttgttcttac tttccatcaa ttcctaaccc atcaacacaa
     3661 taaaaaacca cggggcatcg cagccccgtg gttaaaacaa atgttcagtg atttagtgag
     3721 cgctggaggt cacctgacga cctgccgcgc gatcccagat aatggccaga accaccatca
     3781 ccactgttgg cattaaccac gccagacctt gttcggccag cggtaaacgc tgcgcccagg
     3841 acggtaagat atcgctgaat gcagatgctt tgatcccgtc gagaatacca aaaagcaggc
     3901 tgataaacat cggcggagca atcacgcggg acgaattatg ccaccatgag cgtgtaaaac
     3961 ttaatacaac cagtgcgata cacggcggat aaatagcggt cagcaccggt acggagatct
     4021 ggatcagctg gcttaagccg aggttagaaa ccaccatcga gaagccgccg aggataaaca
     4081 ccagcgtacg ataagagagc ggtacgtact gggcaaagaa ttctgcacaa gcacaggtca
     4141 ggccaactgc cgttaccagg caggcgatga agattaacgc cgccagcagg aagctaccgc
     4201 cgccgccaaa ggtgtgctga acgtaagcat gcagaatagc agcgccgttt gcagactgat
     4261 cgaccagcga cgcgctgtct gaacccagac ggaacagcgc caggtacagc agagtcagac
     4321 caacacccgc catcaggcca gcccagacgg tataacgggt cagtaggcgc gcttcggtaa
     4381 cgccacgaga acgcgccgcg ttaacaataa cgataccaaa caccattgcg cccagcgtat
     4441 ccatggtcag atagccgttg acgaagccgt tagaaaacgc agcgttttga taagcctcag
     4501 tcgccgtgct gatagaaccc gccggccaga caatcgcggc aacagacagg atgaccagcg
     4561 cgataatttt cagcggcgca aggaagttgc ccacggtatc cagcagcttg cccggataga
     4621 gcgaaaccag aataacgata gcgaaataga ccaggctgta gataaacagc ggcagcgcgg
     4681 aatcacccgt cagcggcgca atccccactt caaaagaaac ggtagctgta cgcggcgtag
     4741 cgaaaagcgg ccccaccgcc aggtaacaaa ccgttgccag cagtacgcca gcgactttac
     4801 cgattggcgt gctgaggctg tcaacaccgc cgccaacttt tgccagcgcc actaccgtta
     4861 ataccggcag gccaacggca gtaatgagga agccgaatgc cgcagtccag acgtgttcgc
     4921 ctgcctgtaa gccgaccatt ggagggaaaa taatgttacc tgcgccgacg aacaacgcaa
     4981 atgtcataaa gcccagagcg atgatatcgc gcgatcttaa ttgatgggtc ataaatcata
     5041 ctgcctgtgg atgtggtgtt gtaacgttaa atttttgtgc caatccctgg ggatgataca
     5101 gcggtatatt taaacaattt tagcgggaaa tactcccaac caggcgcggc gaagaatagt
     5161 ttttcgattt accgcgttaa tgcagtcagt atgacagcac ctgaagcgca atttaaacgc
     5221 ttataacgtt ttaaagcaag gagggatcta aaaaccagag gtatatagcg ataagaaatc
     5281 accaaccagt ataaaacact atttccgtga aaaagacatg gctaatcatg cgaacaaaaa
     5341 atcaccaacc gttgtaaaat ctaacgcccg tattttgact gcaaagaaaa taagccagca
     5401 tcggctggct tatggcaata aaagatgaca aaggcggatt aatcgctgtt tttgacaatt
     5461 aaacgttccg ggataacaaa actgaaacgt gttccttttc ctactgtact ctcaatattc
     5521 aggcgacttt cgtgatgatt caccgcatgt ttcacgatcg ctaaccctaa tccgctaccg
     5581 ccggtttgcc gggaacgcgc tttatcaacg cgataaaaac gctcggtcag gcgcggaata
     5641 tgctccggtg caatgcccgg tccgttatct tcaacgctaa attcggcacc gtgcggcact
     5701 cgctgccagc gtacggtgat atgcgtgcct tccggcgtat gattcacggc gttatagacc
     5761 aggttcgaaa tcacactgcg tagctgatct tcgttgccag acaccttgag gccgttatct
     5821 atctcgaagg taaatgtctg ttttttctga ctcagagtct gagcctcgcg ctcaacaacg
     5881 cgcagcatca tcggcacatc aaccttttca ttgagcaatt gcgtcggtgc ggcttcaatt
     5941 ttcgacagcg ttagcaactg cttcaccagc ccttccatcc gctgggtctg ctcgcgcatg
     6001 gtgtgcaacg ctttttcgcg taccgcgcct tccagcggct gctcatccat catctccagg
     6061 taaccctgta acacggtcaa tggcgtacgt aactcatggc tcacgttggc aaaaaagtta
     6121 cgccgcgccc cttccagttg atgcatttgc gtgacatcac gcgccaccat cagcaactgt
     6181 ttgtgggtat aaggcatgac gcgaatttcc agatgccgcc cggtgttgag caccagattg
     6241 agcgggcgag aaaaatcacg cgttttcaaa tattgcgtaa actccgggta acgcagcagg
     6301 ttaaggatgt tctgcccgtt atcttccggc cagcgcaaac caagaatctg ttgcgccaga
     6361 ccgttacacc agaaaatacc gccctcttcc gtggtcagca ccaccgcgtc gggcagcgac
     6421 tccgcgccgc tacgaaagcg tttaatcaga ttgcccagtt cacggcggcg ttttttattt
     6481 cgcagctgca tctggtgtaa gccgtatagt agcggttccc agctaccacg ccccggtggc
     6541 ggggtcatac tgcgatccac ccacagccac catgaaaggc gcaataaatt ccagaaatgc
     6601 cagataagca gtcctgttac cgatgccagc aaaaaccagg gcaggtaacc aaaaaatgca
     6661 cccaggatga aagccgggag gcagcaaagt agcagctcca gcaccagcct tttccacgac
     6721 agccgttcca gcacgtaaga tactccagtt aagaaatcat aagccctgct ctgcgtccga
     6781 tgagcaaggc gttaaaagcg ggttgaaaaa cgatatcctg taccgcgcac ggtctgcacc
     6841 atgcggtcat gcccgccggg ctccagtgct ttacgcaggc gacgaatgtg gacatcgacc
     6901 gtgcggtctt ccacatacac gttagttccc cagacgtggt ttaacagctg ctcgcggctg
     6961 tacacgcgct caggatgcgt cataaaaaag tgcagcagtt taaattctgt cggccccatc
     7021 tccagcggct cttcgcccgc catcactcgg tgagatgtcg ggtcgagact taatccctgc
     7081 atctcaatca cctcttccac cgccattggc gaaatacggc gcattaccgc tttgattcgc
     7141 gccaccagct ccttcggcga aaacggcttg gtgatatagt catccgcgcc ggtttcaagg
     7201 ccgcgcacgc gatcttcttc ttcccctctg gcggtcaaca tcaccactgg aatatcccgg
     7261 gtcatcgact cgcgcttgag gtgtttgatg aactggatac cggagccgcc aggtaacatc
     7321 cagtcaagga gaattaaatc cggccagggt tcattcagtt gattcacagc actgtcataa
     7381 tcttccgctt cgaccggctg aaagccattt tgttcgagca cgaagcagac catttcgcga
     7441 attggagctt catcttctac gaccagaata cgtctcgcca tgatttgccc tgttgtaata
     7501 aataggttgc gatcattaat gcgacgtcat tatgcgtcag atttatgaca gatttatgaa
     7561 aagctcgtcg cacatatctt caggttattg atttccgtgg cgcagaaaaa agcaaatggc
     7621 acatctgttt gggtataatc gcgcccatgc tttttcgcca gggaaccgtt atgcgcatcc
     7681 ttcacacctc agactggcat ctcggccaga acttctacag taaaagccgc gaagctgaac
     7741 atcaggcttt tcttgactgg ctgctggaga cagcacaaac ccatcaggtg gatgcgatta
     7801 ttgttgccgg tgatgttttc gataccggct cgccgcccag ttacgcccgc acgttataca
     7861 accgttttgt tgtcaattta cagcaaactg gctgtcatct ggtggtactg gcaggaaacc
     7921 atgactcggt cgccacgctg aatgaatcgc gcgatatcat ggcgttcctc aatactaccg
     7981 tggtcgccag cgccggacat gcgccgcaaa tcttgcctcg tcgcgacggg acgccaggcg
     8041 cagtgctgtg ccccattccg tttttacgtc cgcgtgacat tattaccagc caggcggggc
     8101 ttaacggtat tgaaaaacag cagcatttac tggcagcgat taccgattat taccaacaac
     8161 actatgccga tgcctgcaaa ctgcgcggcg atcagcctct gcccatcatc gccacgggac
     8221 atttaacgac cgtgggggcc agtaaaagtg acgccgtgcg tgacatttat attggcacgc
     8281 tggacgcgtt tccggcacaa aactttccac cagccgacta catcgcgctc gggcatattc
     8341 accgcgcaca gattattggc ggcatggaac atgttcgcta ttgcggctcc cccattccac
     8401 tgagttttga tgaatgcggt aagagtaaat atgtccatct ggtgacattt tcaaacggca
     8461 aattagagag cgtggaaaac ctgaacgtac cggtaacgca acccatggca gtgctgaaag
     8521 gcgatctggc gtcgattacc gcacagctgg aacagtggcg cgatgtatcg caggagccac
     8581 ctgtctggct ggatatcgaa atcactactg atgagtatct gcatgatatt cagcgcaaaa
     8641 tccaggcatt aaccgaatca ttgcctgttg aagtattgct ggtacgtcgg agtcgtgaac
     8701 agcgcgagcg tgtgttagcc agccaacagc gtgaaaccct cagcgaactc agcgtcgaag
     8761 aggtgttcaa tcgccgtctg gcactggaag aactggatga atcgcagcag caacgtctgc
     8821 agcatctttt caccacgacg ttgcataccc tcgccggaga acacgaagca tgaaaattct
     8881 cagcctgcgc ctgaaaaacc tgaactcatt aaaaggcgaa tggaagattg atttcacccg
     8941 cgagccgttc gccagcaacg ggctgtttgc cattaccggc ccgaccggtg cggggaaaac
     9001 caccctgctg gacgccattt gtctggcgct gtatcacgaa actccgcgtc tctctaacgt
     9061 ttcacaatcg caaaatgatc tcatgacccg cgataccgct gaatgtctgg cggaggtgga
     9121 gtttgaagtg aaaggtgaag cgtatcgcgc gttctggagc cagaatcggg cgcgtaacca
     9181 acccgacggt aatttgcagg tgccacgcgt agagctggcg cgctgcgccg acggcaaaat
     9241 tctcgccgac aaagtgaaag ataagctgga actgacagcg acgttaaccg ggctggatta
     9301 cgggcgcttc acccgttcga tgctgctttc gcaggggcaa tttgctgcct tcctgaatgc
     9361 caaacccaaa gaacgcgcgg aattgctcga ggagttaacc ggcactgaaa tctacgggca
     9421 aatctcggcg atggtttttg agcagcacaa atcggcccgc acagagctgg agaagctgca
     9481 agcgcaggcc agcggcgtcg cgttgctcac gccggaacaa gtgcaatcgc tgacagcgag
     9541 tttgcaggta cttactgacg aagaaaaaca gttacttacc gcgcagcagc aagaacaaca
     9601 atcactaaac tggttaacgc gtctggacga attgcagcaa gaagccagcc gccgtcagca
     9661 ggccttgcaa caggcgttag ccgaagaaga aaaagcgcaa cctcaactgg cggcgctcag
     9721 tctggcacaa ccggcacgaa atcttcgtcc gcactgggaa cgcatcgcag aacacagcgc
     9781 ggcgctggcg catactcgcc agcagattga agaagtaaat actcgcttac agaacacaat
     9841 ggcgcttcgc gcgagcattc gccaccacgc ggcgaagcag tcagcagaat tacagcagca
     9901 gcaacaaagc ctgaatacct ggttacagga acacgaccgc ttccgtcagt ggaacaacga
     9961 actggcgggt tggcgtgcgc agttctccca acaaaccagc gatcgcgagc atctgcggca
    10021 atggcagcaa cagttaaccc atgctgagca aaaacttaat gcgcttgcgg cgatcacgtt
    10081 gatgttaacc gccgatgaag ttgctaccgc cctggcgcaa catgctgagc aacgcccact
    10141 gcgtcagcgc ctggtcgcgc tgcatgggca gattgttccc caacaaaaac gtctggcgca
    10201 gttaatggtc actatccaga atgtcactct ggaacagacg caacgtaatg ccgcacttaa
    10261 cgaaatgcgc cagcgttata aagaaaagac gcagcaactt gccgatgtga aaaccatttg
    10321 cgagcaggaa gcgcgcatca aaacgctgga agctcaacgt gcacagttac aggcgggtca
    10381 gccttgtcca ctttgtggtt ccaccagcca tccggcggtc gaggcgtatc aggcgctgga
    10441 gcctggcgtt aatcagtctc gattactggc gctggaaaac gaagttaaaa agctcggcga
    10501 agaaggtgcg gcgctgcgcg ggcaactgga tgcattaacg aagcagcttc agcgcgatga
    10561 aaacgaagcg caaagcctcc gacaagatga gcaagcactt actcaacaat ggcaagccgt
    10621 cacggccagc ctcaatatca ccttgcagcc acaggacgat attcaaccgt ggctggatgc
    10681 acaagatgag cacgaacgcc agctgcggtt actcagccaa cgacatgaat tacaagggca
    10741 gattgccgcg cataatcagc aaattatcca gtatcaacag caaattgaac aacgccagca
    10801 gcagctctta acggcattgg cgggttatgc actgacattg ccacaggaag atgaagaaga
    10861 gagctggttg gcgacacgtc agcaagaagc gcagagctgg cagcaacgcc agaacgaatt
    10921 aaccgcgctg caaaaccgta ttcagcagct gacgccgatt ctggaaacgt tgccgcaaag
    10981 tgatgatctc ccgcacagcg aagaaactgt ggcgctggat aactggcggc aggttcatga
    11041 acaatgtctc gcattacaca gccagcagca gacgttacag caacaggatg ttctggcggc
    11101 gcaaagtctg caaaaagccc aggcgcagtt tgacaccgcg ctacaggcca gcgtctttga
    11161 cgatcagcag gcgttccttg cggcgctaat ggatgaacaa acactaacgc agctggaaca
    11221 gctcaagcag aatctggaaa accagcgccg tcaggcgcaa acgctggtca ctcagacagc
    11281 agaaacgctg gcacagcatc aacaacaccg acctgacggg ttggctctca ctgtgacggt
    11341 ggagcagatt cagcaagagt tagcgcaaac tcaccaaaag ttgcgtgaaa acaccacgag
    11401 tcaaggcgag attcgccagc agctgaagca ggatgcagat aaccgtcagc aacaacaaac
    11461 cttactgcag caaattgctc aaatgacgca gcaggttgag gactggggat atctgaattc
    11521 gctaataggt tccaaagagg gcgataaatt ccgcaagttt gcccaggggc tgacgctgga
    11581 taatttagtc catctcgcta atcagcaact tacccggctg cacgggcgct atctgttaca
    11641 gcgcaaagcc agcgaggcgc tggaagtcga ggttgttgat acctggcagg cagatgcggt
    11701 acgcgatacc cgtacccttt ccggcggcga aagtttcctc gttagtctgg cgctggcgct
    11761 ggcgctttcg gatctggtca gccataaaac acgtattgac tcgctgttcc ttgatgaagg
    11821 ttttggcacg ctggatagcg aaacgctgga taccgccctt gatgcgctgg atgccctgaa
    11881 cgccagtggc aaaaccatcg gtgtgattag ccacgttgaa gcgatgaaag agcgtattcc
    11941 tgtgcagatc aaagtgaaga agatcaacgg cctgggctac agcaaactgg aaagtacgtt
    12001 tgcagtgaaa taactattca gcaggataat gaatacagag gggcgaatta tctcttggcc
    12061 ttgctggtcg ttatcctgca agctatcact ttattggcta cggtgattgg tagccgttct
    12121 ggtagttgtg atggtggtat gaaaaaagtc attttatctt tggctctggg cacgtttggt
    12181 ttggggatgg ccgaatttgg cattatgggc gtgctcacgg agctggcgca taacgtagga
    12241 atttcgattc ctgctgccgg gcatatgatc tcgtattatg cgctgggggt ggtggtcggt
    12301 gcgccaataa tcgcactctt ttccagccgc tactcactca aacatatctt gttgtttctg
    12361 gtggcgttgt gcgtcattgg caacgccatg ttcacgctct cttcgtcgta cctgatgctt
    12421 gccattggtc gcctggtttc cggctttccg catggcgcat tttttggcgt cggagcgatc
    12481 gtgttatcaa aaattatcaa acccggaaaa gtcaccgccg ccgtggcggg gatggtttcc
    12541 gggatgacag tcgccaattt gctgggcatt ccgctgggaa cgtatttaag tcaggaattt
    12601 agctggcgtt acaccttttt attgatcgct gtttttaata ttgcggtgat ggcatcggtc
    12661 tatttttggg tgccggatat tcgcgacgag gcgaaaggaa agctgcgcga acaatttcac
    12721 tttttgcgca gcccggcccc gtggttaatt ttcgccgcca ccatgtttgg caacgcaggt
    12781 gtgtttgcct ggttcagcta cgtaaagcca tacatgatgt ttatttccgg tttttcggaa
    12841 acggcgatga cctttattat gatgttagtt gggctaggga tggtgctggg aaatatgcta
    12901 agtggcagga tttcaggacg ttattcacca ctgcgcattg cagcagtgac tgactttata
    12961 attgtactgg cactgctgat gctctttttc tgcggcggca tgaaaacaac gtcgcttatt
    13021 tttgctttta tttgttgcgc gggattattt gccctttcag cacctctgca aatattgtta
    13081 ctgcaaaatg ccaaaggcgg agagttatta ggtgccgcag gtgggcaaat agcgtttaac
    13141 ctcggtagcg ccgtcggcgc atattgcgga ggtatgatgc tgacgctggg gctggcatat
    13201 aattacgtgg cgctgcctgc cgccctgctt tcgtttgctg cgatgtcatc gttgctgctg
    13261 tatggtcgct ataagcgcca gcaagcggcg gatagtccgg tgctggcgaa accactgggg
    13321 tagattactg tcacggtgtg cgttgcttat tgccggatgc ggcgtgaacg ccttatccgc
    13381 cctacgcggt tctggcacat tttgtaggcc tgataagacg cggcaagcgt cgcatcaggc
    13441 atgatgcgcc aattgcctgg gttttttact cttgtggcca taaccacgca gcgccgcgta
    13501 cgccgctgga atcaccgtgc ttcgccttac gcaccggcgt ttcacattcg ccgccgaaga
    13561 caaattgttt aatcaactgc ggaaccgttt gatataaacg gtctacattg ctcatcccgc
    13621 cccccaggac aatcacatcc ggatcgagaa tattcacgac atgtgccagc gattttgcca
    13681 gccgcagctc gtagcgacgc aatgccagtt ccgctaccgg atcgctttct tcaaccaggc
    13741 ggataatttc actgcctttc agcgcatgtc cgctcaaacg acgataatcc gtcgcgaatc
    13801 ccgtgcccga aataaaggtt tcaatacaac cttgtttacc gcaataacaa gggacttcct
    13861 cgcgataacg cagttcgtct tcgtccatcc acggtagcgg attgtgtccc cactcacctg
    13921 ccgtgccatt gccgccgata tgcgcccgcc cattgaatgc cacgcccgcg ccgcatcccg
    13981 tgccgataat cacggcaaat accgtctgcg ctcccgctgc cgcgccatct actgcttctg
    14041 aaaccgccag acagttggcg tcatttgcca gccgcacttc ccgctgcaac ctcgcgctta
    14101 agtctttatc gaatggctga ccgttgagcc aggttgaatt ggcattcttc accacaccgg
    14161 tgtaaggcga aattgagcca ggaatgccca tacctaccgt tccgcgctgc cccgtcgcct
    14221 gctccgccat atcaaccaac gtggcgatcg tttcaatagt ctgccggtaa tcatcacgcg
    14281 gcgtgggcag acgatggcgg tacaactgct cccctgcatc gcccagtgca atcacttcag
    14341 ttttggtgcc gcctaaatcg atacctatac gcacggtact ctccttattt ttttcaatat
    14401 caatagcgta gagacggaca accggattgg caatgcaagg ccgccgacaa ttcgttatca
    14461 tgcccgctaa atttaacgac aaggccgtgg aaattatcat gctgtggttc aaaaatttaa
    14521 tggtttaccg tcttagccgc gagatttcgc tgcgtgcaga agagatggaa aaacagctaa
    14581 cctcgatggc atttacccca tgcggcagcc aggacatggc gaagatgggc tgggttcctc
    14641 cgatgggatc gcacagcgat gcgttaacgc acgttgccaa tggtcaaatt gttatctgcg
    14701 cgcgcaaaga agaaaaaatc ctaccgtctc cggtgattaa acaggcgctg gaagcgaaaa
    14761 tcgccaaact ggaagcggaa caggcgcgta agctgaagaa aaccgaaaaa gattcgctga
    14821 aagacgaagt gctgcactct ctgttgccgc gtgctttcag ccgttttagc cagacaatga
    14881 tgtggatcga cacggttaac ggtttgatta tggtggactg cgccagtgcc aaaaaagcgg
    14941 aagatacgct ggcactgctg cgtaaaagcc tggggtcgtt accggttgta ccattgagca
    15001 tggcaaaccc gattgaactg acgctgaccg aatgggtacg ttccggtagt gcggcacagg
    15061 gcttccagct gcttgatgaa gccgagctga aatcgttgct ggaagatggc ggagtgatcc
    15121 gcgcgaagaa acaagatctg accagcgaag agatcaccaa tcacattgaa gccggaaaag
    15181 tggtgactaa actggcgctc gactggcagc agcgcattca gtttgtgatg tgcgacgatg
    15241 gttcgctcaa gcgtctgaag ttctgcgacg agctgcgcga tcaaaacgaa gatatcgacc
    15301 gtgaagattt cgcccagcgt tttgatgccg atttcatcct gatgactggt gaactggcag
    15361 cgttaattca aaacctgatt gaaggattag gtggcgaagc acaacgttaa ttgctgattt
    15421 tcctttaatg ccggatgcga cgcctgccgc gtcttatccg gcgtacgaag ccacaccagg
    15481 catataatta ttcgctacgg cgggcaataa ttttttagcg cagcaatatt atgcgtttta
    15541 cgccgtaact gatctaatcg cctcatccct attcgcctgt cagcagcacg cagaatattc
    15601 actaaccccc atttatcgct ggtaaattgt tttgttaaca actccgcttt tggggtgtca
    15661 atatattcac gcaataaatc agaaattgaa caaatatccg tcaggtaagg ataccagcaa
    15721 gtttcaccgt gaaaattacg cactccaccg ttgcctgcaa taaagtcttt cgggtaatcc
    15781 cagatcacgt ttttatccaa cgtgatccaa taacgcggta aatcggtgct tccaccattt
    15841 tggctgcgca tgggataacg ggtacagtga atttgcaggt tgatcgttgg cgtcagtaag
    15901 tcatagatct cccgctgtaa cttgctccag gggcgttgtg tcattgtttt tcctcaagcc
    15961 ggtgacgcgc ttcattgcga attttccgcg ttgccccggt tttcactaaa tcccgtaact
    16021 gcgaaagatc gggtttatcg acaaataaaa gtaatgatgg atcctctttt agccacaccg
    16081 ttttcacatc ggcagccagc tgtggattat taatagccag cgaccgatcc tgagactcag
    16141 tcaatgtcgt taacaacgac gccggggtat gtgcgttgcg taatgccgtt tggcgcaaat
    16201 gatcattgcg actttgcagc aatgcacgcc accattcatc atctgattta tcttttatcg
    16261 tcgccagttc cgcaaaaata tcgctatcct catggttcca gcggtgtaat cctctttcac
    16321 tttgtctacg ggtgctattt ttcatactga caacagccag cgcccagtca gagttgaagc
    16381 gatactgttg atcgagtttg ttgataattc cggaaggtaa ttctttatta tcaaataatt
    16441 ggttacgcat tcgatagcgg tattcagcgg gtaaatcaac cagtaaatca atggcattat
    16501 ttaacatctc ttcgtcaaca acaccattat caatcagcgc cagctgtact tcgtaggctt
    16561 gctcttgatc ttcttgccgt ttgctcatca ttaaatttaa tgtgcgttgc cataactgtt
    16621 tcttatattc cagcggcaat aatttatcgc gtgccagctc atcccagacg gtgggaacat
    16681 catgctgagt caatagataa ctaatcacgt ccttcgatgt cagataacgc gcaccttcgc
    16741 gcaaattgtg cacatcttct tttgccagag acagttgacg catctccggc aacgctatca
    16801 ataacacctt tacgatattt ttattttctt tattatccag atacatttgt tctgcgatac
    16861 gttcaatatc ctctgtacgc agggttgctg acagttgggt aatttttaat tcagtaagcg
    16921 tccgggctaa tgcttcacga actttcagtg atttatcttt actcaacatc actgcttgtt
    16981 gtgcggttaa atctgctccg gtaattcttt cacgtaactc cgggtcttcg ttatttatca
    17041 actcatcggc cactgcagtc ggaaccagcc aggcgtaatc gtaatggctg agtaatgtct
    17101 tgcgtacact gagaaagggg ctatgactta acgacgcaac aatatctgga gaaagattat
    17161 cctgtctggc ctgctccagc tgtacgctct cgacgggtac agacattaat tttttattca
    17221 catcggcgga aatattactg tttttaacca gccctctggc aacagcctca tctttaatta
    17281 attcatcggt aagccaatgc atctgttgag cgggccagga ctgcgccatc gattcacgcg
    17341 tctggtcatc ggggtgctgt aacagttgca gcataacggc gggtgataaa ctctctgtat
    17401 gagtcagaaa acgtgccacc agtggatctt gatgctttat tttattcgct gggaaattat
    17461 cgagtttccc cgtttcaatc cattttaatt tctcattaat ttcaggagcg gctttatctc
    17521 gaaaaagcgg taaaccctga acgatgttgt ctgcgacgct atcatagtca ggatacgcat
    17581 tggaaatgtt atcggtttcg atttcagtgt gatcataaag atcggcagtc accagtgaat
    17641 aggctttttg aatattctca tcccagttat atccttcacc acttccatct tcattgatat
    17701 aaaattcagc tttaatgact tcaaattgcg ttgctttatc ggaaacaata atgcctcgcg
    17761 cactaattcc cttcatcact tcaaagttat gatcattgta atcagcataa actaaacctt
    17821 tcgctgtcat ctgtcctttg acatacgcga aatcatgatt aataaagtta tcgacaataa
    17881 cattacctaa tacaatcaga tgaccattac ctgacagata atcatcataa tcgccggaaa
    17941 tcgttaaatt accgttcacc accagtttat ttttattgct gaagttagcg ggtaatgata
    18001 ggtcaccatt atgataatac cagcggatat tatcttttag catataagca gcttccatac
    18061 tatcgtcggt tgccgcttta accacgttgc gctcatctgc cgtcataaga tgaaagtcag
    18121 aggtgataag gtcctcgcta ctgatccact ctgtggcaca tgcattgcca gtcataaaaa
    18181 ataagaccat acagatgacc aatatattaa ttctattcat aatcttcaat gatatttata
    18241 tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa aggaaatatt
    18301 attatggggt tatgagagga catttcagaa aaatgccctc ccgttcaagc ggaaggcgag
    18361 gaattacaga tagcggcaca gataagaggt gggttcggca acttgcagat gaaactcact
    18421 gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg tcgcgtcagg
    18481 cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag cggtgctgaa
    18541 ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc tgctggaaaa
    18601 gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa actggccctg
    18661 tctggtagtt gaatatctac ttcactatag gggccagatg cgctggctgt cacgcaaaat
    18721 tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa gacaggaaca
    18781 tcgagctgtt tttgcagtaa atcgcgatga cgctggttaa atcccaaaca atccagcatg
    18841 atgacatctg cgcctttcgc cagtaattct ttcccggcat caatgatttt ttgttctgaa
    18901 tcatgaatgg ggttacccaa tgaaaataca ggcggtttct gcaaaatttg ccatttttgc
    18961 gcctgaacgg tcagcaactc ctcaaccggt acgataaccc ccacctgatg atcttcaaca
    19021 atagaggaaa ccagcggtgg caatatccgc gacggctcaa gaaagatcgt attacgcgca
    19081 gtcatactac taatgtttgc cgtactcatt aatataatga cgtcgtagcc ctgattatcg
    19141 agcacttcaa ccacaccttg caggtcacgc tctactttgc gacgcgaaac atgggccaga
    19201 tggttgtcat ttaataatgt gagaattgtg tcttcgcctg cttctggcgc gtactccgcc
    19261 atcacttctt cgcggcttaa cttccccagc aggctatgat gggaaatatt atcttcgtca
    19321 atgtattctg tcaggagcgg caaaacttcc tgcataggta caatgccgat ggttagaatc
    19381 gccaacgacg cactcattgt tcttccacct tacttttatt tcgtcgttaa ttaccgggca
    19441 agtgttaagc accactgctg acacttactt gtgctacaaa tgacaaaaag cgtagcagca
    19501 gacgtgcacg gcataatgta aagattccaa atgattccag taatggattg ttattgttta
    19561 atattctaat tatttgaaaa acatgaatta tgaaaaatgt gacgcagatc ccttgtagtg
    19621 gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg tcctcatagc
    19681 gttttttcgc atccatcgct tcctgagcgg tcggatgttc actgatcaac gagtctggtt
    19741 taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt cctttttcaa
    19801 ttgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt cttccttatt
    19861 tcacgggatg aacgttaagt ataggcgctc gaaaatcaac aattgatcgt ctgcgccagg
    19921 gcgctgcgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat gataatatgc
    19981 gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc ttcgctcagc
    20041 ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag tcggttaacc
    20101 aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat gaagtgacga
    20161 ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgg cgcagttacc
    20221 gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct ttcgacgatc
    20281 tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac aaaccgacgg
    20341 ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg aggcccgatc
    20401 agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg gcgaaagccg
    20461 gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt aaagaaaaaa
    20521 ttccacttaa agtgaaaatc tcaatacatc ccttactata ccaataaata ttcaagaatg
    20581 aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc caccacttct
    20641 ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt ccgccggtct
    20701 ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc tcacgaatgg
    20761 tcgctggcgt gtaacgttca ccgcgcggat taagcgccgc agcgcctttc tcgatggctt
    20821 cagcagccaa aggtatatct gcggtgatca ccaaatctcc cgcttcacac tgccggacaa
    20881 tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata aatcgcgatg
    20941 gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata cgttccgccg
    21001 cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc caaattgtca
    21061 taaagtcatc ctttgttggg taatcctcta ttgtgtcgcg cttttgcctt ccggcatagt
    21121 tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag gagtgaggca
    21181 atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat tctcggcggt
    21241 ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatatacccc ctccccggat
    21301 aaagtcgccg ccctgcatga caagttcggc atcaacgccg cagaatcggc gcaagaagtg
    21361 gcgcaaatcg ccgacatcat ttttgccgcc gttaaacctg gcatcatgat taaagtgctt
    21421 agcgaaatca cctccagcct caataaagac tctctggtcg tttctattgc tgcaggtgtc
    21481 acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg cgccatgccg
    21541 aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc gctggtaacc
    21601 ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc ggaagtaatt
    21661 gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc ctacgtcttt
    21721 atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg cgcccaggcg
    21781 tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga aacgggagaa
    21841 catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat tgaagcggta
    21901 cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac gaagtgtatg
    21961 gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag gccgccactt
    22021 cggtgcggtt acgtccggct ttctttgctt tgtagagcgc caaatctgcc gatttcaacc
    22081 actcgcgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta atccgtaaat
    22141 ttacctgtgg cgtattcggc aaacgtaatg tatttagccc ttcatgcacc cgtaacatgg
    22201 cggtaatggc gctctcagct ggcgtaccgg acatgattac cgcaaactca tcgccgccaa
    22261 accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa
    22321 tcgcttcatc gcccacatca tggccccagg tatcgttgat gctcttgaaa tggtcgatat
    22381 cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat tcattgcgta
    22441 acatagtttc ccaatgacgt cggttattca cgcccgtcat gccgtcgcgg gtactcatga
    22501 cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg acccagccaa
    22561 acagcagagg ataaatgaca ataatgggaa gggagagcca ccattccagc ggcgcactat
    22621 tgaacgacac ggtaatgccc gtcagctcga gggtgacaag gcaggaaacc accatcaaca
    22681 ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga cacataatca
    22741 tcaacatcgc ggtggaaggc agcacgttta cgcccattac gcctacccac attcccgcta
    22801 atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg gccctgctcg
    22861 ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc agccaccacc
    22921 agcccggcgg cgggtgtgaa accagcgttg aagcaatcgg taagaacatg ccagccaggc
    22981 caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt
    23041 tttgaggagt taaaggaggt tcctccccgt gcgccgccgc ttttttgaaa aagttttcat
    23101 cattcattat ttttgggaac attcagaaac aattttccca aattatagag acggggctaa
    23161 aggcagaagt atgaaatttc gggtgagcca ttttagtagc tcacccgtta gcactatcag
    23221 gtatcaggcg gctttcttga ggcaggcgct cataaactta ttacgatcat cacctttcag
    23281 tgattgttgc gttgcttgat tattacattc gcgcatcttt tgctgctgtg gcgtcaaact
    23341 tttttcacca ggcgcagact tgctgttctt caggcaatca ctcatgtagg tcttacgagc
    23401 atcccctttc aacgcctgtg ccgtcgcctg ctgattacag gaggtcatac gctgttgttg
    23461 tggggttaag gttctctcgg cagcgccgac ggtggttaaa aaaaccagac cgaaaagcaa
    23521 ggtaaccagt aatgtaattt tcatagcacc atccctcttc atgttttaac catgagcgta
    23581 tgcgcccgtg atctgacatt aagtctggtt gctaacagca aaaaaaccac ccggcagcga
    23641 aaattcactg ccgggagcgg ttttatttca gccccagagc ggctttcatg gtgtagaaga
    23701 gatcggtctg gtcggtcagt ccaacgacat tggcggcatg cgggccatac gccgcaatac
    23761 gcaactgact gccggtatgt tcttatgaat cctcttcgga gttcccgtaa ctcatcacca
    23821 tcactgcgcc atctttggta tttagcgcct gagtgaggcc cggagctttg gtatccggcg
    23881 caacaatctg gctggcgtgg gcgtgatcag cggtgactat gaccagcgtg ttaccatcct
    23941 ttttggcgaa ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt
    24001 gcccacaagg attcgcagca tgatcctgtt tatcgattga cgcaccttca acttgcagga
    24061 aaaagccttt ctcattttta ctcaacaatt caatggcttt gtcggtcatc tgtgccaggg
    24121 tcggtacact gtcattacgt tgcggattag gcgtacaggt gactgcgggc ttatcgatat
    24181 taccgtggta cgttgctttc ggtcctagcc agcgcactgg catattgccg tcagcaaaca
    24241 ggccaagcag gggtttttgc tgattcgctt ccgtcaccga attcagtgag gcagcatcgc
    24301 tcaccaactg ataaccacgc gcctgtgcct gttcacgcag cgtttttccc tgccattcac
    24361 cagcggttgc cgtttcagca aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag
    24421 cgttaagcag ctgttcggta atcgatcctt ttccgccttt ttccagagcg ttacccggac
    24481 atttttcact ggtcgcgctc ggaccgtagc atttgcgcga ggtcacatgc gccaccagcg
    24541 cggcgggcgt ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc agacctgcgg
    24601 cttttgccat ttccagaatc gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc
    24661 cgttataggt tttgacaccg gttgaccagg cggttgctga tgcagccgag tcggtgacgt
    24721 agtccggttt tccggttttt ttattcagcg catagtgagt gtattgcccg gtaagcggta
    24781 aggcatcgat acctttaaaa aagccgcccg caccttcggc ataattacgt gcggcagtaa
    24841 tttccgaatc ccccatccca tcgccaatca gcaaaataat attttttgca ggtttatcgc
    24901 taagagaatc acgcagagcg gcggtctgat cagccgttaa gcggcgagca ccgccgggtg
    24961 cagtaatatc gccctgagca gcccggtttt ccagaacagg catttctggt gtccgggctt
    25021 ttgtcacagg ggtaaacagt aacggtaaga gtgccagtgc aatagtgctt tgtttcactt
    25081 t
//
LOCUS       DAZFDC010000066        24052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFDC010000066 DAZFDC010000000
VERSION     DAZFDC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.066"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            168..503
                     /locus_tag="ACHJ48_002751"
     CDS             168..503
                     /locus_tag="ACHJ48_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO4708276.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            762..1205
                     /gene="yjdN"
                     /locus_tag="ACHJ48_002752"
     CDS             762..1205
                     /gene="yjdN"
                     /locus_tag="ACHJ48_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO4708277.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1338..2126
                     /gene="phnC"
                     /locus_tag="ACHJ48_002753"
     CDS             1338..2126
                     /gene="phnC"
                     /locus_tag="ACHJ48_002753"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO4708278.1"
                     /translation="MQTIICVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMAHFAYQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2151..3167
                     /gene="phnD"
                     /locus_tag="ACHJ48_002754"
     CDS             2151..3167
                     /gene="phnD"
                     /locus_tag="ACHJ48_002754"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4708279.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFSKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAMQ"
     gene            3273..4052
                     /gene="phnE"
                     /locus_tag="ACHJ48_002755"
     CDS             3273..4052
                     /gene="phnE"
                     /locus_tag="ACHJ48_002755"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO4708280.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4073..4798
                     /gene="phnF"
                     /locus_tag="ACHJ48_002756"
     CDS             4073..4798
                     /gene="phnF"
                     /locus_tag="ACHJ48_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO4708281.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4799..5251
                     /gene="phnG"
                     /locus_tag="ACHJ48_002757"
     CDS             4799..5251
                     /gene="phnG"
                     /locus_tag="ACHJ48_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO4708282.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITANYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5248..5832
                     /gene="phnH"
                     /locus_tag="ACHJ48_002758"
     CDS             5248..5832
                     /gene="phnH"
                     /locus_tag="ACHJ48_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO4708283.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5832..6896
                     /gene="phnI"
                     /locus_tag="ACHJ48_002759"
     CDS             5832..6896
                     /gene="phnI"
                     /locus_tag="ACHJ48_002759"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO4708284.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDESPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6889..7734
                     /gene="phnJ"
                     /locus_tag="ACHJ48_002760"
     CDS             6889..7734
                     /gene="phnJ"
                     /locus_tag="ACHJ48_002760"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO4708285.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7731..8489
                     /gene="phnK"
                     /locus_tag="ACHJ48_002761"
     CDS             7731..8489
                     /gene="phnK"
                     /locus_tag="ACHJ48_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO4708286.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8600..9280
                     /gene="phnL"
                     /locus_tag="ACHJ48_002762"
     CDS             8600..9280
                     /gene="phnL"
                     /locus_tag="ACHJ48_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO4708287.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9277..10404
                     /gene="phnM"
                     /locus_tag="ACHJ48_002763"
     CDS             9277..10404
                     /gene="phnM"
                     /locus_tag="ACHJ48_002763"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO4708288.1"
                     /translation="MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLM
                     DHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAMCRA
                     RQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGS
                     HSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKNPAQ
                     ALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10404..10961
                     /gene="phnN"
                     /locus_tag="ACHJ48_002764"
     CDS             10404..10961
                     /gene="phnN"
                     /locus_tag="ACHJ48_002764"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO4708289.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10948..11383
                     /gene="phnO"
                     /locus_tag="ACHJ48_002765"
                     /pseudo
     CDS             10948..11383
                     /gene="phnO"
                     /locus_tag="ACHJ48_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
     gene            11385..12143
                     /gene="phnP"
                     /locus_tag="ACHJ48_002766"
     CDS             11385..12143
                     /gene="phnP"
                     /locus_tag="ACHJ48_002766"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO4708290.1"
                     /translation="MSLTLTLTGSGGAQGVPAWGCECAACARARRSPHYRRQPCSGVV
                     KFNEAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12220..12549)
                     /gene="yjdP"
                     /locus_tag="ACHJ48_002767"
     CDS             complement(12220..12549)
                     /gene="yjdP"
                     /locus_tag="ACHJ48_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO4708291.1"
                     /translation="MKRHSTLFLFTLLTLTTVPAQADIIDDTIGNIQQAINDASNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQHQLNQERRQLEDEERR
                     MEDEYGQ"
     gene            complement(12618..14888)
                     /locus_tag="ACHJ48_002768"
     CDS             complement(12618..14888)
                     /locus_tag="ACHJ48_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4708292.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDDILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LNDFNDFNDEMRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSG
                     QRVAQSYQDLRSGERVIMIFALLAVVITAFAGWYIYRNLGTNLTAISRAMSRLAHGEA
                     DVTFPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEAL
                     LHSQKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNPDSPDAPRIQRALQAAERGALLT
                     QRLLAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLALEIEAQSPAWPAWIDISQL
                     ENAIINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGCGMSQAVK
                     SQVFEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAA
                     KNLSPAAVEQASVSGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAA
                     SAEIDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLR
                     KPFTRAQLAQALGQEN"
     gene            15002..16522
                     /locus_tag="ACHJ48_002769"
     CDS             15002..16522
                     /locus_tag="ACHJ48_002769"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO4708293.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLSVAENI
                     FLGSELSHGGLVQRKEMLVQAQKVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSSL
                     LVRSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAAENITMATLERDARWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPARGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVYVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            16519..17493
                     /locus_tag="ACHJ48_002770"
     CDS             16519..17493
                     /locus_tag="ACHJ48_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO4708294.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFITPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVVCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSASRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAMIIIAVLIDKYRTRHHQSA"
     gene            17512..18453
                     /locus_tag="ACHJ48_002771"
     CDS             17512..18453
                     /locus_tag="ACHJ48_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO4708295.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            18508..19191
                     /locus_tag="ACHJ48_002772"
     CDS             18508..19191
                     /locus_tag="ACHJ48_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO4708296.1"
                     /translation="MKRSAINDILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGRNNFAAHGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTADSDITVVIDGCRQKHTAGSQLRLSPG
                     ESICLPPGLYHSFWAEAGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFR"
     gene            19206..20066
                     /locus_tag="ACHJ48_002773"
     CDS             19206..20066
                     /locus_tag="ACHJ48_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFO4708297.1"
                     /translation="MPLISLAEGLAHAREHHYSLGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARHDIPVVLNLDHGLHFESVVQALRLGFSSVM
                     FDGSTLGYEENVRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGISDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            20063..21064
                     /locus_tag="ACHJ48_002774"
     CDS             20063..21064
                     /locus_tag="ACHJ48_002774"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO4708298.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLPLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHYPEGAWGETPEQGGVWIPSQKLEQREIIGS
                     VGAGDAFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(21061..21768)
                     /locus_tag="ACHJ48_002775"
     CDS             complement(21061..21768)
                     /locus_tag="ACHJ48_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO4708299.1"
                     /translation="MKPVVLVVDDDTAICALLQDVLSEHVFTVSVCHTGQEAILRIEG
                     DPDIALVVLDMMLPDTNGLRVLQQIQKLRPTLPVVMLTGMGSESDVVVGLEMGADDYI
                     CKPFTPRVVVARLKAVLRRVGALAVNDEKSAGLSFNGWYLDTMRCQLHNPLQQHVELT
                     QGEYGLLLALAQNARRVLSREQLLAFTHSDSVEVFDRTIDVLIMRLRRKIELNPHQPM
                     LIKTLRGLGYVFAADVH"
     gene            22059..24044
                     /gene="yjcS"
                     /locus_tag="ACHJ48_002776"
     CDS             22059..24044
                     /gene="yjcS"
                     /locus_tag="ACHJ48_002776"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO4708300.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTITFIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5355 a         7011 c         6735 g         4951 t
ORIGIN      
        1 gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga aaacgatcgt cgtctaaaat
       61 cagcagtacc cccgacaaac tcagggattt tgtgtataat tgcggccttt ttcggcaatc
      121 tgccgttttt tggcgctttt gccctgctga cttttgagga aatccacatg tcattaccac
      181 actgcccaaa atgcaactcc gaatacactt acgaagataa cggcatgtac atctgcccgg
      241 aatgtgccta cgaatggaac gacgcagaac ctgcacagga aagcgacgag ctgatcgtta
      301 aagatgctaa cggcaatctg ctggctgacg gcgacagcgt taccatcatt aaagatctga
      361 aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa agtgaaaaac atccgcctgg
      421 ttgaaggcga ccataacatc gattgcaaaa tcgacggttt tggtccgatg aaactgaaat
      481 ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta agccggataa ggcgtttacg
      541 ccgcatccgg caacagtgcc agatgcctga tgcgacgctc gcgcgtctta tcaggcctac
      601 aaattcccgc accctccgta ggccggataa ggcgtttacg ccgcatccgg caacagtgcc
      661 gactgcctga tgcgacgctc gcgcgtctta tcaggcttcc ctctcatctg tataaatttc
      721 gaactacact taactggctt ctcttaactg aggtcaccat catgccgtta agtccctacc
      781 tctcttttgc cggtaactgc tccgacgcga ttgcctatta tcaacgtacg ttgggcgctg
      841 aactgctcta taaaatcagc ttcggcgaaa tgccaaaatc agcgcaggac agcgccgaga
      901 actgcccttc cggaatgcaa tttcccgata ccgccatcgc ccacgccaat gtgcgcattg
      961 ccggaagcga tatcatgatg agcgatgcta taccgtcagg aaaagccagc tactccggtt
     1021 ttacgctggt gctcgattcg caacaggtcg aagaaggaaa acgctggttt gataaccttg
     1081 ccgctaacgg aaaaatcgaa atggcctggc aggaaacttt ctgggcgcat ggctttggca
     1141 aagtcaccga taaatttggc gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc
     1201 aataatccgc cgggaggccc gccctcccgc actgtcatcg aattcccgtt aactcttcat
     1261 ctgttagtca cttttaatta accaaatcgt cacaataatc cgccacgatg gagccacttt
     1321 tttagggagg ctgcatcatg caaacgatta tctgcgtcga acaactgagc aaaaccttca
     1381 accagcatca ggcgctgcac gcggttgatc tgaatatcca tcatggtgaa atggtggctc
     1441 tgcttgggcc gtccggttcc ggcaaatcca cccttttacg tcacttaagc ggtttgatta
     1501 ccggcgataa atccgccggt agccatatcg agctgctggg ccgcactgtc cagcgcgaag
     1561 gccgcctggc ccgcgatatc cgcaaaagcc gcgccaacac cggctacatc ttccaacaat
     1621 tcaacctggt gaatcgcctg agcgtactgg agaacgtgct gattggcgcg ctcggcagca
     1681 cgccgttctg gcgcacctgt tttagttggt tcacccgcga gcagaaacag cgcgcattac
     1741 aggcgctgac ccgcgtcggt atggcgcact ttgcgtatca gcgtgtttcc acgctctccg
     1801 gcggccagca gcagcgtgtg gcgattgccc gcgcgctgat gcagcaggcg aaagtgattc
     1861 tcgctgacga accgatcgcc tcgctggacc cggaatcggc gcgcatcgtg atggacaccc
     1921 tgcgcgacat caaccagaac gacggcatca ccgtggtcgt cacgctgcat caggtggatt
     1981 acgccctgcg ctactgcgaa cgcatcgtcg ccctgcgcca ggggcacgtc ttctacgacg
     2041 gcagcagcca acagtttgat aacgaacgtt ttgaccatct ctaccgcagc atcaaccgcg
     2101 tcgaagagaa cgcgaaagct gcctgacatc cccatcattg aggaaaacga atgaacgcta
     2161 agataattgc ctcgctggcc ttcaccagca tgttcagcct cagcaccctg ttaagcccgg
     2221 cacacgccga agagcaggaa aaggcgctga atttcggcat tatttcaacg gaatcacagc
     2281 aaaacctgaa accgcaatgg acgccgtttt tacaggatat ggagaagaag ctgggcgtga
     2341 aggtcaacgc cttctttgcc ccggactatg cgggcattat ccagggaatg cgtttcaaca
     2401 aagtggatat cgcctggtac ggcaacctgt cggcgatgga agcagtggat cgcgccaacg
     2461 gccaagtctt cgctcagacc gtcgcggcgg atggatcgcc gggttactgg agcgtgttga
     2521 tcgtcaacaa agacagcccg atcaacaacc tgaacgatct gctggcgaag cggaaagatc
     2581 tcaccttcgg taatggcgat cctaactcca cctctggctt cctcgtcccc ggctactacg
     2641 tcttctccaa aaacaatatc tccgccagtg acttcaagcg caccgtcaac gccgggcatg
     2701 aaaccaacgc gctggccgtc gccaacaagc aggtggatgt ggcgaccaac aacaccgaaa
     2761 acctcgacaa gctgaaaacc tccgcgccgg agaagctgaa agaactgaaa gtgatctgga
     2821 agtcaccgct gatcccaggc gatccgatcg tctggcgtaa aaacctttcc gaaaccacca
     2881 aagacaagat ctacgacttc tttatgaatt acggcaagac gccggaagag aaagcggtgc
     2941 tggaacgcct gggctgggcg ccgttccgcg cctccagcga cctgcaactg gtgccgattc
     3001 gccagctcgc actgtttaaa gagatgcaaa gcgtgaaagg caataaaggg ctgaatgagc
     3061 aggacaagct ggcaaaaacc accgagattc aagcgcagct ggatgacctg gaccgcctga
     3121 acaacgcgtt aagcgcgatg agttcggtaa gtaaagcgat gcagtaaatc gtaggtcgga
     3181 taagacgccc cggtgtcgca tccgacaatg tgcaggcgtt gatgccggat gcggtgcaag
     3241 caccttatcc ggcctacaga ccggagccaa acatgcaaac catcaccatc gccccaccca
     3301 agcgcagctg gttctcgctt ctgagctggg ccgttgtact cgccgtgttg gtcgtctcgt
     3361 ggcagggcgc ggaaatggcc ccgctcacgc tgatcaaaga cggcggcaac atggcgacct
     3421 tcgccgccga cttcttcccg cccgatttca gccagtggca ggattacctc accgaaatgc
     3481 tcgtcacgct gcaaatcgcc gtctggggca ccgcgctggc ggtggttctc tccatcccct
     3541 tcggcctgat gagcgccgaa aacctggtgc cgtggtgggt ttaccagccc gtgcgccgcc
     3601 tgatggacgc ctgccgcgcc attaacgaaa tggtcttcgc catgctgttc gtggtcgccg
     3661 tcggcctcgg cccattcgct ggtgtgctgg cgctgtttat ccacaccacc ggtgtgctct
     3721 ccaaactgct ttccgaagcg gtggaagcga ttgaacccgg cccggtggaa ggcattcgcg
     3781 ccaccggcgc caacaagctc gaagagatcc tctacggcgt gctgccgcag gtgatgccgc
     3841 tgctgatctc ctactccctc tatcgcttcg aatccaacgt ccgctcggcg accgtcgtcg
     3901 gcatggtcgg cgcgggcggg atcggcgtca cgctgtggga agcgattcgc ggtttccagt
     3961 tccagcaaac ctgcgccctg atggtgctga ttatcgtcac ggtcagcctg ctggatttcc
     4021 tctctcaacg gttgcgtaag cactttatct gattagcgag gcattgatag ctatgcactt
     4081 gtctacacat ccgaccagct acccaacacg ctatcaggag atagccgcaa aacttgagca
     4141 ggagcttcgt caacactacc gctgcggcga ctatcttccc gccgagcagc agctggcggc
     4201 gcgctttgag gtaaatcgcc acaccctgcg ccgcgccatc gaccaactgg tggaaaaagg
     4261 ctgggtacag cgccgtcagg gcgtcggcgt gctggtgctg atgcgcccgt tcgattaccc
     4321 gctcaacgcc caggcgcgtt ttagccagaa tctgctggat cagggcagcc atcccaccag
     4381 cgaaaaactg ctgtctgtgc tgcgcccggc ctccggtcac gtcgctgacg cgctggggat
     4441 aaccgagggg gagaacgtca tccatctgcg caccctgcgc cgggtcaacg gcatcgcgct
     4501 ctgtttaatc gaccactact tcgcggacct caccctctgg ccgacgctgc aacgcttcga
     4561 cagcggctcg ctgcacgatt ttctgcgcga gcaaacgggg attgcactgc gccgcagcca
     4621 gacgcggatc agcgcccgcc gcgcccaggc caaagagtgc cagcgtcttg aaattccgaa
     4681 tatgtccccg ctgctgtgcg tgcgcaccct taaccaccgt gacggtgaaa gcagcccggc
     4741 ggagtactcc gtcagcctga cgcgcgccga catgattgaa ttcactatgg agcactgaat
     4801 gcacgcagat accgcgaccc gccagcactg gatgtccgta ctggcacaca gccaaccggc
     4861 agaactggca gcccgcctga acgcgctgaa catcaccgcc aactatgagg tgatccgcgc
     4921 cgctgaaact ggcctggtac agattcaggc gcggatgggc ggcaccggcg aacgcttttt
     4981 tgccggcgac gccacgctga cccgcgccgc cgtacgtctg actgacggca ccctcggcta
     5041 cagctgggtg ctggggcgcg acaaacagca cgccgaacgc tgcgcgctga ttgacgcgct
     5101 gatgcagcaa acccgccatt ttcaaaactt atcagaaacg cttattgccc cgctggacgc
     5161 tgaccgtatg gcacgcattg ccgcacgcca ggccgaagtg aacgccagcc gggtcgactt
     5221 ctttacgatg gttcgcggag acaacgcatg accctggaaa ccgcttttat gcttcccgtg
     5281 caggatgccc agcacagttt tcgtcgcctg ttaaaggcca tgagcgagcc gggcgtgatt
     5341 gtcgccctgc atcagctcaa acgcggctgg cagccgctga atatcgccac caccagcgtg
     5401 ctgctgacgc tggccgataa cgacacgccg gtgtggcttg ccgcgccatt gagcaacgat
     5461 atcgtcagcc agagcctgcg ttttcatacc aacgcgccgc tggtcagcca gccggaacag
     5521 gcgaccttcg cggtaacgga tgaggcgatt tccagcgaac agcttaacgc cctttccacc
     5581 ggcaccgccg ttgcgccgga agcgggcgca acgctgattt tacaggtcgc cagcctgagc
     5641 ggcgggcgca tgctgcgtct caccggcgcg ggtattgccg aagaacgaat gatcgccccg
     5701 cagctgccgg agtgcattct gcacgaactc accgagcgcc cgcacccgtt cccgctcggc
     5761 atcgacctga tcctgacctg cggcgagcgc ctgctggctg ttccgcgaac cactcatgtg
     5821 gaggtgtgct gatgtacgtt gccgtgaaag ggggcgagaa ggcgatcgac gccgcccacg
     5881 ccctgcaaga gagccgacgc cggggcgata ccgatttgcc tgaactgagc gtcgcccaga
     5941 ttgaacagca gcttaatctt gcggtggatc gcgtgatgac cgaaggcggc attgccgacc
     6001 gcgagctggc ggcgctggcg ctgaaacagg ccagcggcga taacgtcgaa gcaattttcc
     6061 tgctgcgcgc ctaccgcacc acgctggcga agctcgcggt aagcgagccg ctcgacacca
     6121 cggagatgcg tctcgaacgg cgtatttcag cggtttataa agatattccc ggcggccagc
     6181 tgcttggccc aacctacgac tacacccatc gcctgctcga tttcaccctg ctggcaaacg
     6241 gcgaagcgcc gacgctgacc accgccgaca gcgaacagca gccgtcgccg cacgttttca
     6301 gcctgctggc gcgtcagggg ctggcgaagt ttgaagagga tagcggcgca cagccggatg
     6361 acatcacccg cacgccaccg gtttacccct gctcacgttc ttcccgcctg caacagctca
     6421 tgcgcggcga cgaaggctat ttgctggcgc tggcctactc cacccaacgc ggttacgggc
     6481 gcaatcaccc gttcgcgggt gaaatccgca gtggttacat cgacgtgtcg attgtgcccg
     6541 aagagctggg atttgcggtg aacgtcggcg aactgctgat gactgagtgt gaaatggtca
     6601 acggttttat cgacccaccg gatgaatctc cgcacttcac gcgcggctac gggctggtgt
     6661 tcggcatgag cgagcgcaaa gcgatggcga tggcgctggt cgaccgcgct ctgcaagccc
     6721 cggagtacgg cgagcacgcg acaggcccgg cgcaggatga agagttcgtg ctggcacatg
     6781 ccgacaacgt cgaagccgca ggctttgtct cgcacctcaa actcccgcac tacgtcgatt
     6841 tccaggccga actggagcta ctcaaacgtc tgcaacagga gcagaaccat ggctaatctg
     6901 agcggctaca actttgccta cctcgacgag cagaccaaac gcatgatccg ccgcgccatc
     6961 ttaaaagcgg tggcgatccc cggttatcag gtgccgtttg gcgggcgcga gatgccaatg
     7021 ccgtacggct ggggaaccgg cggcattcag cttaccgcca gcgtgattgg cgaaagcgac
     7081 gtgctgaagg tgattgacca gggcgcggat gacaccacca acgccgtgtc gattcgcaac
     7141 ttctttaagc gcgtgaccgg ggtaaacacc accgagcgca cggacgatgc aacggtcatc
     7201 cagacgcgtc accgcatccc cgaaacgccg ctgaccgaag atcagatcat tattttccag
     7261 gtgccgatcc ccgaaccgct gcgctttatc gagccgcgcg aaacggaaac ccgcaccatg
     7321 cacgcgctgg aggaatacgg cgtgatgcag gtgaagctgt atgaagatat cgcccgcttc
     7381 ggtcatatcg ccaccaccta cgcctatccg gtgaaggtga acgggcgcta cgtaatggac
     7441 ccgtcgccga tcccgaaatt cgataacccg aaaatggaca tgatgcccgc cctgcaactc
     7501 ttcggcgccg ggcgtgagaa gcgcatctat gcggtgccgc cgtttacccg cgtggaaagc
     7561 ctcgatttcg acgatcaccc gttcaccgtt cagcagtggg atgagccatg cgccatctgc
     7621 ggatcgaccc acagctatct tgatgaagtg gtgctggatg acgccggaaa ccgcatgttt
     7681 gtctgctccg ataccgatta ttgccgccaa cagagcgagg caaaaagcca atgaatcaac
     7741 cgttactttc ggtcaataac ctgacccacc tttacgcgcc gggcaaaggc tttagcgatg
     7801 tctcttttga tttatggccg ggggaagtgc tgggcattgt cggggaatcc ggctccggga
     7861 agaccacgct gctgaaatca atttccgcgc gtctaacgcc gcagcagggg gagattcact
     7921 atgaaaaccg ttccctctat ggcatgagcg aggccgaccg ccgtcgcctg ctgcgcaccg
     7981 aatggggcgt agtgcatcag catccgctcg acggcctgcg ccgccaggtc tcggcaggcg
     8041 gcaatatcgg cgagcggctg atggcgaccg gggcgcgtca ttacggcgat attcgcgcca
     8101 ccgcgcagaa gtggctggaa gaggtggaaa ttcccgccaa ccggatcgac gacctgccga
     8161 ccaccttttc cggcggtatg cagcagcgtt tgcagattgc ccgcaacctg gtgacgcatc
     8221 cgaagctggt gtttatggat gaaccgaccg gcgggctgga tgtgtcggtg caggcccgcc
     8281 tgctcgacct gctgcgcggc ctggtggtgg agctgaacct cgcggtagtg attgtcaccc
     8341 acgatttagg cgtcgcccgc ctgctggcgg accgtttgct ggtgatgaag caggggcaag
     8401 tggtggagag tgggttaacc gaccgcgtgc tcgacgaccc gcatcatccg tatacacagc
     8461 tgctggtgtc atcggttttg cagaattgag ccggtgccgg atgcggcgta aacgccttat
     8521 ccggcctaca aatgcgctcc ccgtaggtcg gataagacgc atcagcgtcg catccgacac
     8581 ccgaaccacg aggcgaaaaa tgattaacgt acaaaacgtc agtaaaacct tcatcctgca
     8641 ccagcaaaac ggcgtgcgct tgcccgtcct caaccgcgcc tcgctcaccg tcaacgcggg
     8701 cgaatgcgtg gtgctccacg gccactccgg cagcggcaaa tcaaccctgc tgcgctcgct
     8761 gtacgccaac tatctgcccg atgaagggca aatccagatc aaacacggtg acgagtgggt
     8821 agacctggtc actgcgccag cgcgcaaagt ggtggaaatc cgcaaaacca ccgttggctg
     8881 ggtgagccag tttctgcgcg ttatcccgcg catttcggcg ctggaagtag taatgcagcc
     8941 gctgctcgat accggcgtac cgcgtgaagc ctgcgccgct aaagccgcgc gtctgctcac
     9001 ccgtctgaac gtgccggaac gcctgtggcg ccttgcgcca tcgaccttct ccggcggcga
     9061 acagcagcgc gtcaacatcg cccgtggctt tatcgtcgac taccccattc tgctgctcga
     9121 cgaacctacc gcctcgctgg acgccaaaaa cagcgccgcc gtagtcgaac tgatccgaga
     9181 agccaaagcc cgcggcgcag ccatcgtcgg cattttccat gacgaagccg tacgtaatga
     9241 cgtcgccgac cgcctgcacc caatgggagc ctcttcatga ttatcaataa cgtcaaactg
     9301 gtgctggaaa acgaagtggt aaacggttcg ctggagatgc aggacggcga aatccgcgcc
     9361 tttgccgaaa gccagagccg cctgccggag gcgatggacg gcgaaggcgg ctggctgctg
     9421 ccggggctga ttgagctgca tacagacaac ctcgataaat tcttcacccc gcgcccgaaa
     9481 gttgactggc ccgcccactc ggcgatgagc agccatgacg cgctgatggt ggcgagcggc
     9541 atcaccaccg tactggacgc cgtggcgatt ggcgacgtgc gagacggcgg cgatcggctg
     9601 gagaatctgg agaagatgat caacgccatc gaagagacgc agaaacgcgg cgtcaaccgc
     9661 gccgagcacc gcctgcacct gcgctgcgag ctgccgcata acaccacgct gccgctgttt
     9721 gaaaagctgg tgcagcgcga gccggtgacg ctgatggatc actcgccggg ccagcgccag
     9781 ttcgccaacc gcgagaagta tcgcgaatat tatcagggca aatactcgct caccgatgcg
     9841 cagatgcagc agtatgaaga ggaacagctg gcgcttgccg cacgctggtc gcagccgaat
     9901 cgcgaatcca tcgccgcaat gtgccgcgcg cgacaaattg cgctcgccag ccacgatgac
     9961 gcaacgcacg cccacgttgc cgaatctcac cagcttggca gcgtgatcgc cgaatttccc
    10021 accacgtttg aagcagcgga agcctcgcgc aagcatggca tgaacgtgct gatgggcgcg
    10081 ccaaatatcg tgcgtggcgg ttcgcactcc ggcaacgtgg cggccagtga actggcacag
    10141 cttggcctgc tggatatcct ctcttccgac tactaccccg ccagcctgct ggatgcggcg
    10201 ttccgcgtcg ccgatgacca gagcaaccgc tttacgctgc cgcaggcggt gaagctggtg
    10261 accaaaaatc cggcgcaggc gcttaatctc caggatcgcg gggtgattgg cgagggcaaa
    10321 cgcgccgacc tggtgctggc gcatcgcaag ggcaatcaca ttcatatcga ccacgtctgg
    10381 cgtcagggta aaagggtgtt ctgatgatgg gaaaactgat ttggttaatg gggccgtccg
    10441 gctccgggaa agatagcctg ctggcggaac tccgcctgcg ggaacaaact cagctactgg
    10501 tggcgcatcg ctacatcacg cgcgacgcca gcgccggaag cgaaaaccat atcgccctga
    10561 gcgagcagga attttttacc cgcgcggggc aaaatctgct ggccttaagc tggcacgcta
    10621 acggtctgta ttatggcgtc ggcgtcgaga ttgacctctg gctgcacgct ggattcgacg
    10681 tgctggtcaa cggctcacgc gcccatctgc cgcaggcgcg ggcgcgctat caatcggcgc
    10741 tgctgcccat ttgcttacag gtttcgccag agatcctgcg ccagcgcctg gaaaaccgtg
    10801 gccgcgaaaa cgccagtgaa attaacgccc ggctggcgcg cgccgcccgc tatactccac
    10861 aggattgcca tacgctcaat aatgacggca gcctgcgcca gtcggtcgac acgctgctga
    10921 cgctaatcca tcagaaggag aaacaccatg cctgcttgtg agctttcgcc cggccacgca
    10981 gtacgacacc gacgcggttt acgcgttgat ttgcgagcta aaacaggcgg agtttgacca
    11041 ccacgcgttt cgcgtgggct ttaacgccaa cctgcgcgac ccgaacatgc gctaccatct
    11101 ggcgctgctg gatggcgaag ttgtcggtat gatcggcctg catttgcagt ttcatctgca
    11161 tcatgtcaac tggatcggcg aaattcagga gttggtggta atgccgcagg cgcgcggtct
    11221 gaacgtcggc agtaagttac tggcgtgggc agaagaagaa gcccgccagg ccggggccga
    11281 aatgaccgaa ctttcgacca acgtgaagcg ccacgacgcg caccgtttct atctgcgcga
    11341 aggctacgag cagagccact tccgcttcac caaggtgctg taacatgagc ctgaccctca
    11401 cgctcaccgg ctccggcggc gcacagggcg ttccggcatg gggctgcgag tgtgcggcct
    11461 gcgccagagc gcggcgctcg ccgcactatc gccgccagcc atgcagcggc gtagtgaagt
    11521 ttaacgaggc gatcaccctg atcgacgccg ggcggcacga tctcgccgat cgctggtcgc
    11581 ccggatcgtt ccagcagttt ttgctgacgc actaccatat ggatcacgtg caggggctat
    11641 tcccgctgcg ctggggcgtg ggcgatccga tcccggttta cggcccaccg gatgaacagg
    11701 gctgcgacga tctgtttaaa catccgggcc tgcttgattt cagccacacg gtggaaccgt
    11761 ttgtggtgtt tgatttgcag gggttacagg tcacacccct gccgctcaac cactcaaaac
    11821 tgaccttcgg ttatctgctg gaaacggcgc acagccgggt ggcgtggctg tctgacaccg
    11881 ctggcttgcc ggaaaaaacg ctgaaatttt tacgcaataa tcagccgcag gtaatggtga
    11941 ttgattgcag ccacccgccg cgcgaggatg caccgcgtaa tcactgtgat ttaaataccg
    12001 tgcttgcgct gaatcaggtt atccgctcgc cgcgcgtggt tctgacccat atcagccacc
    12061 agtttgatgc gtggctgatg gaaaacgcac tgccgtcggg gtttgaggcg gggtttgatg
    12121 ggatggagat tggggtggcg tgatgagagg gaatgtgcgt gctggccccc tcaccctaac
    12181 cctctcccca gaggggcgat gcctgcccca tacccaacat cattgcccat actcatcttc
    12241 cattctccgc tcttcatctt ctagctgccg acgctcctga ttaagctggt gctggcgatc
    12301 gtccagctgc ctgcgacgat cttcaaactg gcgacggcgg tcgtcatatt gtctgcgccg
    12361 atcgtcgctc acttcacgct gccagccgtc gtcgcgcgaa tcttcataat ctcgcccacg
    12421 gtcagggtta gaagcgtcgt taatcgcctg ctgaatattg ccaatggtgt catcgataat
    12481 atcggcctgg gccggtacgg tggtcagcgt gagcagggtg aaaagaaaaa gcgtggagtg
    12541 gcgtttcata aactcggcct cgcaacaaag ttaatacaag gtaggcaaag gtgcctgtta
    12601 cgagtagagg aggattctca attctcttgc cccagcgcct gcgccaactg cgcacgggta
    12661 aagggtttgc gcaacagcgc cacgtctggc agcgcggggt tatggctggg gcgtaaatcc
    12721 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    12781 tctgcgccgc tcatgccgcc ggggagcatt aaatcgctga taaaaatatc gatttctgct
    12841 gatgccgcca gcagatccag cgcctgttcg ccgctgcttg cttccagcgt caaatagccc
    12901 agcaggtgca actgttcgca gatggtctgg cgcaccgcgg cttcatcttc cagcactaac
    12961 accagtttat cgccgctgac agaagcctgt tctactgccg ctggcgatag gtttttcgcc
    13021 gccgtcagcg cgcggggaag ctgtaatctg accgttgtcc cctgccccgg cgcgctttca
    13081 atttcaacgc gcccaccgga ctggcgcaca aaaccataga ccatcgacaa acccagcccg
    13141 ctgccgctgc ccgtctgttt ggtggtaaag aatggctcaa atacctggct tttcaccgcc
    13201 tgcgacatac cgcagccatg atcgatgacc tccagtgcca ccatgtcctg acgctgccca
    13261 ctgctgcgag taacgcgctg attccaggta cgaattttga tgattcccgt ctgcccttcc
    13321 atcgcgtcac gggcgttcat caccaggttg atgatggcat tttccagttg gctgatgtca
    13381 atccacgcgg gccacgccgg agactgcgct tcaatctcca gcgccagcgt tgccggtaag
    13441 gagtggcgca ttaattcgct taactcttcc agcaatgttt tcagttcaac ggcttgcgga
    13501 tgtaaggact gcttgcggga aaacgccagc agtcgttgtg tcagtagcgc cccacgttcg
    13561 gcagcttgca gcgcccgctg tattcgcggc gcgtctggcg agtccggatt caccagctcc
    13621 aggctgccaa taatcaccgc cagcagatta ttaaaatcat gggccagccc gcccgtcagt
    13681 tgcccgacag ctttcagttt ctggctgtgt aatagcgcct cttccaaccc ctgccgctca
    13741 gtttgggcaa tctccatctg cgtggtcttc tcttttagca gccgtgaggt tcgttcgagt
    13801 gacgcggtat tgcgggcaaa aacgttaaag gcgcgcgcca gttcacccaa ctcatcacgc
    13861 cgctgtaacg ccggaaaagt gacgtccgct tcgccgtgcg ccaggcggct catggcgcgg
    13921 gaaatcgccg tcaggttagt gccgaggttg cgatagatat accagccggc aaacgcggtg
    13981 atcaccaccg ctaacagggc gaaaatcatg atcaccctct cgccggagcg caaatcctgg
    14041 taactttgcg ccacacgctg accggagagc gtcgccacct gttcggcgta acggttgatg
    14101 tcgccattga ggatcgccac cagcgctttg atgtgaaaca tataccagct gattgccaga
    14161 tcgctttgct ccagagcggc agaaagcggc gcgagtttac gcatttcatc attgaaatca
    14221 ttgaaatcat tgagtaacgt attaaccaat ggcgtatcgc tgtgggtcgg caacgcagac
    14281 attaccccga ccagttgatg aataatcgcc tgcggcgcgg gggtggcaat agcggcaaca
    14341 atcagtcggt tcatctcgtt aagcaaaata tcatcttgcg cgtgagtgac tttttgcaga
    14401 tgccgcagat aacttaagtt ttgatacagc gaacttaaca gcgcgttgcg ctccagatgc
    14461 cgcctttgcc cgcgctccag cataccgccg acgctggttt gcagttcgtt gctacgcgtg
    14521 atgattcgcg tcaccagccc ggcttcctgc tgcgctaaag gcgcgttcgc cagttgtgca
    14581 agcgagcttt ttaatgcccg ctgtgtctcc tgcaaccgct cggcttcgcc tttatattcc
    14641 agcgctccca ccacctgcga caatcgcacc gccgccgttg ccacgtttgc cgtgtcgcgc
    14701 gccagattca ggctgccggt catatcatcc accgtttgct gctgcaccag ctcctgcatc
    14761 tggctggcat gacgaaagcc cattaccgcc acgccgctga ccatcaacgt caccgccacc
    14821 accagcaaat tcaaaagcag caatcgcccg cgcgcgctgg caaaaaagtg acgtccgggg
    14881 tgcgccatat cacgctccgt gaagaaaaat cgcaatatga caaatatgaa cggcttctga
    14941 cattttgcat ttactttcct gtgatggagt gctgatatcg acaacctcgc aggagtccac
    15001 catgaacagc acgccagttc tggagatgcg caatattgcc aaagccttcg gcaaatttta
    15061 tgcactcaaa ggggtggatt tgacggtcta ccctggcgag atccatgccc tgatggggga
    15121 aaacggcgcg ggaaaaagca cgctgatgaa ggtgctggcg ggggcgtata ccgccaccag
    15181 cggcgagatt ctcatagacg gcaagccctt tcacattcgc acgccaaaag atgccttaag
    15241 cgccggtatt acgctgattt atcaggagat gcagctggca ccgaatctgt cggtggcaga
    15301 aaatattttt ctcggcagcg agctttccca cggcgggctg gtgcagcgta aagagatgct
    15361 agtgcaggcg caaaaagtga tcgaccgcct cggcgcgcag tttaacgcca gcgataaggt
    15421 catgacgctg accattgccg agcaacagca ggtcgaaatc gcccgcgcac tacatcgcaa
    15481 cagccgcatt ctggtgatgg acgaacccac cgctgccctc tcctcccgcg aaactcaccg
    15541 cctgtttgaa ctgattatgc ggctgcgcga tgaagggatg gcgattatct acattagcca
    15601 ccgcatggcg gaagtgtatg agctttccga tcgcgtcagc gtgctacgcg acgggcaata
    15661 cgttggcagc ctgacccgcg ataacctcaa tgccggggag ctggtgcgga tgatggtcgg
    15721 caggccactg agcgatctgt tcaataaaga gcgcgatatc ccgctcggta aagcccgcct
    15781 gaatgttcac cacctgaccg acggcggcaa agtccagccg agtagcctgc tggtgcgttc
    15841 cggcgaaatt gttggcctcg ccggactggt gggtgccgga cgttccgaac tggcgcagtt
    15901 gatcttcggc gtgcggaaag cgacaggcgg aatgattgaa gtcgatggtg aaccggtggt
    15961 gatccactcc ccgcgcgaag ccatcgatct tggcattggt tttctcaccg aaaaccgcaa
    16021 agaacaaggc ttattccttg aaatggcagc agccgaaaac atcaccatgg caaccctgga
    16081 gcgcgatgcc cgctggggaa tgctcaatcg caaaaaagcg caaaccattt ccgatgacgc
    16141 cattaagttg ctcaacattc gcgtgcctca tgcccaggta cgcgcgggcg ggctttccgg
    16201 cggcaatcag caaaaactgt tgatctcccg ctgggtggcg attggcccgc gcattttact
    16261 gctcgatgaa cccgcccgcg gcgtggacgt tggcgccaaa agtgagatct accggatcat
    16321 gaacgagatg gcgcgcaagg gcgtggcgat cctgatgatc tccagcgaac tgccggagat
    16381 agtgggaatg agcgatcgcg tctatgtgat gcgcgaaggc agcattgccg gtgagttaaa
    16441 cggcaaaaac atcacccagg aaaacattat gaccttagcg actggcgtca acgacgccca
    16501 ttcccaggcg gtaacctcat gacgacctct gcaaaaacct ctgccaaaaa aatgctgatg
    16561 agcgatctga tgcaaacggt cggcatcttg cccattttga ttctgatcgt ggcggtattt
    16621 ggctttatca cgccaaactt ttttaccgaa agcaacctgc tgaatattac ccgccaggca
    16681 tcgatcaaca ttgttctggc ggcggggatg accttcatca ttttgaccgg cgggatcgat
    16741 ctttccgtgg gttcgatcct cggcaccaca gcggtggcgg cgatggtcgt ctcccttagc
    16801 cctgagttcg ccatgctgtc gatcccggca gcgctgatgc tcggcctgct gctggggctg
    16861 ttcaacggcg cgctggtggc ctttgccgga ttgccaccgt ttatcgttac gcttggcacc
    16921 tatacggcgc tgcgcggggc ggcgtatctg ctggcggacg gcaccacggt tatcaactcc
    16981 aacattaatt tcgagtggat aggcaacaac tatctcgggc cggtcccatg gctggtggtg
    17041 attgcgctgg cggtgatcgt ggtgtgctgg tttattctgc gccgcaccac gcttggcgtt
    17101 catatctacg ccgtcggcgg caatatgcag gcggcgcgtc ttaccggcat taaggtctgg
    17161 ctggtgctgc tgtttgttta tggcatgagc ggcctgcttt ctgggcttgg tgggattatg
    17221 agcgcctcac ggctgtacag cgccaacggc aacttaggca tgggttacga actggatgcc
    17281 attgcagcgg tgatcctcgg cggcaccagt tttgtcggcg gcatcggcac catcaccggc
    17341 acgctggtcg gcgcgctaat tatcgccacg ctcaacaatg gcatgacctt aatgggcgtc
    17401 tcttacttct ggcaactggt gatcaaaggg gcgatgatca tcattgcggt gctgatcgac
    17461 aaataccgta cccgacacca tcaaagtgca taaacacatg aggaatacag catgagattg
    17521 aagcccttag tgaccgcgct ctgtgcaggc gcgctgctgg ccgcagcacc gttcgcccag
    17581 gcgaaagaac tgaaatcaat aggcgtaacg gtgggcgatc tggcgaaccc gttcttcgtg
    17641 cagatcacca aaggcgcgga gctggaagca cgcaaactgg caggcgataa cgtcaaagtg
    17701 acgctggttt ccagcggcta cgatctgggt cagcaggtgt cgcagataga caactttatc
    17761 gccgctaacg tcgatatgat tatcctcaac gctgccgatt caaaagggat cggcccggcg
    17821 gtgaaacggg cgaaagatgc cgggatcgtg gtcgtcgcgg ttgacgtagc ggcagaaggt
    17881 gccgatgcca ccatcacttc cgataacacc caggcaggcg aaatggcctg taaatacatc
    17941 accgatcgcc tgaaaggcaa aggcaacgtg gtgatcatca acggcccgcc ggtatccgcc
    18001 gtgcaaaacc gcgtcgaagg atgccagact gaatttaaac gccatcctga catcaaagtg
    18061 ctctctgaca accagaacgc gaaaggcagt cgtgaaggcg ggctggaggt aatgacggga
    18121 ctgctggcgg caaatccgaa gatcgacggc gtatttgcga ttaacgatcc caccgcaatc
    18181 ggtgccgatc tggcagcaaa acaggcacaa cgtaacgagt tctttattgt cggtgtggac
    18241 ggaagcccgg acgccgaaga ggcgctcaag cgtgagaact cgttgtttgt cgctactccg
    18301 gcgcaagatc cccaggtgat ggcagcgaaa gcggtggaaa ttggctacga cattttgcag
    18361 ggcaaacctg cgccgaaaga gccggtgctg atcccggtga cgatgatcga taaaaacaac
    18421 gtcggcaatt acaaaggctg gacggtgaaa taagtccaca cgccgggcgg cgcagctgcc
    18481 cggtcaccgc taaggagaac gctttcgatg aaacgctccg ctataaacga cattctcggc
    18541 catacacggc aattcttttc gcaacatgac gtacatctgc cgccctttgc cagtttctcg
    18601 cctgctcaat ggcagcagct cgacaccgcc gcgtgggagg aggtttttga tcttaaactc
    18661 ggctgggatg ttaccgcttt tggtcgcaac aactttgcag cacacgggct gacgctgttt
    18721 accctacgta acggctcagc aaaaggaatg ccgtatgtga aatgttacgc cgagaagatc
    18781 atgcatgtgc gcgatgcgca ggtcacgcca atgcattttc actggcgtaa gcgtgaggac
    18841 attatcaatc gcggcggcgg taatttaatt gttgaactgt ggaatgccga tagtaacgag
    18901 caaacggctg atagtgacat tacggtggtg atagacggct gccgccagaa acatactgca
    18961 ggctctcagt tgcgcctgtc tcccggcgaa agtatctgcc tgccgcccgg cctgtatcac
    19021 agcttttggg cagaagccgg ttttggcgat gtgctggttg gcgaagtctc ttctgttaat
    19081 gacgacgacc acgataacca ctttttacag ccactggacc gctacaacct gattgacgaa
    19141 gacgaacctg cacagttggt attgtgtaac gaataccgcc agttccgctg aataaggata
    19201 acgttatgcc attgatttct ctcgctgaag ggctggcaca tgcccgcgaa catcactatt
    19261 cgttaggtgc gtttaatgtg ctggactcgc actttttacg cgccttgttt gctgctgcca
    19321 gacaagaacg ttcgcccttt attatcaaca ttgccgaagt acattttaaa tatatctcgc
    19381 tggattccct ggtcgagaca gtgaaatttg aagcggcgcg tcatgacatt ccggtggtgt
    19441 taaatctcga tcacggactg cattttgaat cggtggtgca agcgttgcgg ctgggattca
    19501 gctcggtaat gtttgatggc tccacgctgg ggtatgaaga aaatgtccgc cagacgcggg
    19561 aagtggtgaa aatgtgccat gccgttggcg tatcggtaga agctgaactg ggcgcggtgg
    19621 gtggcgacga aggcggtgca ctctatggtc atgccgatga atccctgttt accgatccgc
    19681 aactggcgcg tgattttgtc gaccgtaccg gcattgacgc gctggccgtg gcgattggca
    19741 atgcacacgg caaatatcgc ggcgaaccga aactcgattt cccgcgcctc gacgctatcc
    19801 gccagcaggc tgctatcccg ctggtattac acggcggctc agggattagc gatgccgatt
    19861 tccgccgcgc catcgaactg ggtatccaca aaatcaattt ctacactggc atgtcgcaag
    19921 cggcgctggc tgccgttgag gcccgaatgg cgcaccgtca gccgatgtat gacgagtttg
    19981 ccgagttact gctggcagtg gaagccgcca tcagcgatac ggttgcacaa cagatgcgta
    20041 tttttggcag tgcggggcag gcatgatgga gcgtaaaggg attatcgccg caggcaatat
    20101 gttggttgac cacgtccatc agattatcca gtggccagag cgcggctggc tggcggagat
    20161 cacccacagc gaacgctcca ccggcggtgc gccgcttaac gtcctgctga cgctggcgaa
    20221 aatgcgcacc gggctgccgt tacaggcggt tgggctaatc ggtcaggaca gcgacggcga
    20281 ttacatcatg gcgatgcttg agcaatatca cgtcaatcgt cagcatgtgc agcgcactac
    20341 ctttgccccg acgtcgatgt cgcaggtgat gacagaccca agcgggcagc ggacgttttt
    20401 ccattcgccg ggtgcaaaca gacttttgga tcttccggcg ttcgatcagc tcgataacac
    20461 catgaagatt ttccatctcg gctatttact gttgctcgac agcctggata tgccggacga
    20521 tgtttacggc acccgtagcg cacgcctgct ggcgcaaatg cgtgatactg gctttgaaac
    20581 ctcactcgac ctggtctccc gtaagggcga tccgcgttat cagccgctgg tgctgcccgc
    20641 gttgcgccat ctggattatc tggtaattaa cgaactggag gccggggagt ttagcgggct
    20701 ggaaattcgg ttacccaacg gcgaaccgca tatcgcgcat attgctgccg ccgcgcgtga
    20761 actgttagat gcgggcgtgc gccagcgggt ggtgatccac tacccggaag gggcgtgggg
    20821 agagacccca gaacagggcg gcgtgtggat cccttcgcaa aagttagaac aacgggaaat
    20881 tatcggcagc gtcggcgcgg gcgatgcatt ttgtgccgga tttttatatg gctgccatga
    20941 acgctggacg cttaccgaga gcatcaaact ggcacacgcc tgcgcgcggg caagcctgtt
    21001 atgcgccaat gccatcgacg gggcgaaaac gctggaagag ctgcaaacag aaatgtcgga
    21061 ttaatgcacg tcggcagcga agacataccc cagaccgcgc agagttttaa tcaacatcgg
    21121 ctgatgcggg ttgagttcga ttttgcgtcg taagcgcatg attaatacgt cgatggtgcg
    21181 atcaaacacc tccacgctgt cgctgtgggt gaacgccagc aactgctcgc ggctcaatac
    21241 ccgacgcgcg ttttgcgcca gagccagtaa caagccatat tcgccctgag ttaactcgac
    21301 atgttgctga agcggattgt gcaactggca gcgcatggta tcgagatacc agccattgaa
    21361 tgacagcccg gcgcttttct cgtcattaac cgccagcgcg ccaacccgtc gcaacaccgc
    21421 tttcagtcgt gcgaccacca cccgcggggt gaacggtttg cagatgtaat catccgcgcc
    21481 catttccagc ccgaccacca catcagactc actgcccatg ccggtcaaca tcaccaccgg
    21541 caatgtcgga cgcagtttct ggatttgctg caataccctc aggccgttgg tatcgggtag
    21601 catcatatcc agcaccacca gcgcaatgtc aggatcgccc tcaatacgca atatggcctc
    21661 ctgtccggta tggcagactg aaaccgtaaa gacatgttca cttaacacgt cctgcaacag
    21721 tgcgcagata gccgtgtcat catccaccac cagcacgacg ggtttcatgg ttacactccg
    21781 catcattcat ctgattaatc attgtgcgta cttcttgtcg aagtgaaagc tgaaagtaat
    21841 ataaataata aaaatataag tcaggtcaca ttattgatga tagtaataaa tatgtcgtac
    21901 ttttccctct tcagcacaaa tgcctaataa aatggatcgt tccgctcaca cttccactta
    21961 ttaaagtcgc aatttcccaa ttatgataac gccagcttta aatgacgtat tttgcatgga
    22021 ttacatattt acgacataaa tgataaatgg agatttatat gaataactct cggttattcc
    22081 gtttgagcag gattgttatt gcgttaactg ccgccagcgg catgatggta aataccgcta
    22141 acgcgaaaga ggaagcgaaa gccgccactc aatataccca acaggttaat cagaattacg
    22201 ccaaatcatt accgtttagc gatcgtcagg attttgacga tgcccagcgt ggatttatcg
    22261 ccccgctgct ggatgaaggt attctgcgtg atgcgaacgg taaagtttac taccgcgcag
    22321 acgattacaa atttgatatt aatgccgctg cgccggaaac cgtaaacccc agcctgtggc
    22381 gtcagtcgca gatcaacggc atttctggcc tgttcaaagt caccgataaa atgtatcagg
    22441 tgcgcggcca ggatatctct aacattacgt tcgttgaggg ggagaaaggc attattgtca
    22501 tcgacccgct ggtgacgccg cctgccgcaa aagccgcact tgacctttac ttccagcatc
    22561 gtccgcaaaa accgattgtt gccgttatct acactcacag ccacaccgac cactatggtg
    22621 gcgtgaaagg cattatctct gaagccgatg ttaaatccgg caaagttcag gtgattgccc
    22681 ctgctggctt tatggacgaa gccatcagcg aaaacgtgct ggcgggtaac atcatgagcc
    22741 gccgtgcgct ctactcttac ggtctgttac tgccgcacaa cgcgcaaggc aacgtgggta
    22801 atggccttgg cgtgacgctg gcaacgggcg acccgagcat tattgcaccg actaaaacta
    22861 tcgtcagaac tggcgagaag atgattatcg acggcctgga gtttgacttc ctgatgaccc
    22921 caggtagcga agcgccagcc gaaatgcact tctatattcc ggccctgaaa gccctgtgta
    22981 ccgccgagaa cgccacgcat accctgcaca acttctacac tctgcgcggc gcgaaaaccc
    23041 gcgataccag caagtggacc gagtacctga acgaaacgct ggatatgtgg ggtaacgacg
    23101 cggaagtact gtttatgccg cacacatggc cggtctgggg caataagcat atcaatgatt
    23161 atattggtaa atatcgcgat actatcacgt tcattcacga ccagaccctg cacctggcga
    23221 accagggcta caccatgaat gaaatcggcg acatgattaa gctgccgcct gcacttgcca
    23281 ataactgggc cagccgtggc tattacggtt ctgtcagcca caacgcccgc gcggtgtata
    23341 acttctatct tggctattac gacggtaacc cggctaacct gcatccgtat ggtcaggtag
    23401 agatgggcaa acgttacgtg aaagcgctgg gcggctctgc ccgtgtcatc aacctggcgc
    23461 aagaagcgaa taagcaaggt gattaccgct ggtcggcaga actactgaaa caggtgattg
    23521 ccgccaaccc gggtgaccag gtggcgaaga atctgcaagc gaataacttt gaacagctgg
    23581 gctatcaggc cgagtccgcc acatggcgcg gtttctacct gaccggcgcg aaagagctgc
    23641 gcgaaggggt gcataagttc agccacggca ccaccggttc cccggacacc attcgcggaa
    23701 tgtcggtcga aatgctgttc gactttatgg ccgttcgcct cgatagcgcg aaagctgcgg
    23761 gtaaaaatat cagcctgaac ttcaatatga gcaacggcga taacctcaac ctgacgctga
    23821 acgatagcgt gcttaactac cgtaaaacac tgcaaccgca agccgacgcc tctttctaca
    23881 tcagccgtga agatctgcac gccgtgctga ccggacaggc aaaaatggcg gatctggtga
    23941 aagcgaagaa agccaaaatt attggcaatg gcgcgaaact ggaagaaatt atcgcctgtc
    24001 tggataattt cgatttgtgg gtgaatatcg taaccccaaa ttaatgtcac tc
//
LOCUS       DAZFDC010000067        24048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFDC010000067 DAZFDC010000000
VERSION     DAZFDC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.067"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            102..3854
                     /gene="yfaL"
                     /locus_tag="ACHJ48_002777"
     CDS             102..3854
                     /gene="yfaL"
                     /locus_tag="ACHJ48_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO4708301.1"
                     /translation="MRVIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFAHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDAGAVLSLEGDAADLAALQDDPQSIVLNGGMLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVSLAND
                     NQYLGTTQIASGTLMVSDNSQLGYTHYNRQVIFTDKPQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNCDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTQDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGKQKQGAWLDNWLQYA
                     WFSNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDASTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3995..4717)
                     /gene="ubiG"
                     /locus_tag="ACHJ48_002778"
     CDS             complement(3995..4717)
                     /gene="ubiG"
                     /locus_tag="ACHJ48_002778"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO4708302.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4864..7491
                     /gene="gyrA"
                     /locus_tag="ACHJ48_002779"
     CDS             4864..7491
                     /gene="gyrA"
                     /locus_tag="ACHJ48_002779"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO4708303.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7699..9327
                     /gene="yfaA"
                     /locus_tag="ACHJ48_002780"
     CDS             7699..9327
                     /gene="yfaA"
                     /locus_tag="ACHJ48_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO4708304.1"
                     /translation="MALLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIASDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPVSQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMP
                     KLDALSQQPRYVMKLAQMEPGAAWQWLPIIWLPL"
     gene            9324..9947
                     /gene="yfaT"
                     /locus_tag="ACHJ48_002781"
     CDS             9324..9947
                     /gene="yfaT"
                     /locus_tag="ACHJ48_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO4708305.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9971..14486
                     /locus_tag="ACHJ48_002782"
                     /pseudo
     CDS             9971..14486
                     /locus_tag="ACHJ48_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            14487..16136
                     /gene="yfaQ"
                     /locus_tag="ACHJ48_002783"
                     /pseudo
     CDS             14487..16136
                     /gene="yfaQ"
                     /locus_tag="ACHJ48_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            16141..16917
                     /gene="yfaP"
                     /locus_tag="ACHJ48_002784"
     CDS             16141..16917
                     /gene="yfaP"
                     /locus_tag="ACHJ48_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO4708306.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17192..20041
                     /gene="rcsC"
                     /locus_tag="ACHJ48_002785"
     CDS             17192..20041
                     /gene="rcsC"
                     /locus_tag="ACHJ48_002785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO4708307.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLMIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20241..20891)
                     /gene="rcsB"
                     /locus_tag="ACHJ48_002786"
     CDS             complement(20241..20891)
                     /gene="rcsB"
                     /locus_tag="ACHJ48_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO4708308.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20908..23580)
                     /gene="rcsD"
                     /locus_tag="ACHJ48_002787"
     CDS             complement(20908..23580)
                     /gene="rcsD"
                     /locus_tag="ACHJ48_002787"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO4708309.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5876 a         5850 c         6550 g         5772 t
ORIGIN      
        1 tagctattgc atcgggttgg acaatttaca aaagaaagat aacccatatt gtataatgcg
       61 tttaaatatg ccgtcttata taaaatgatg gtcagattaa tatgcgggtt atctttctac
      121 gcaaggagta tttatctcta ctcccgtcaa tgattgcatc tcttttctct gctaacggtg
      181 tcgcggcggc cattgattta tgccagggat atgatatcaa agcgagttgt cacgccagca
      241 ggcaaagcct ttcaggcatt acgcaggtct ggagtattgc cgatgggcaa tggctggttt
      301 tttcggatat gaccaataat gccagcggtg gggccgtatt tttgcaacaa ggagcggaat
      361 ttacattatc accagaaaat gaaactggaa tgactctgtt tgccaataac accgtttcag
      421 gagaatataa taacggcggg gcaatatttg ctaaagaaaa ctcaacgctg aatcttacgg
      481 atgttatttt ttctggtaac gtcgcaggcg gctatggtgg cgcaatctat tcttctggta
      541 ctaacgatac cggtgccatc gatttacgtg tcactaacgc cgtgtttcgc aataacatcg
      601 ctaatgacgg caaaggtggt gcaatttata ccatcaataa tgatatctat ttaagtgatg
      661 atgtttttaa caataaccag gcatatacat caacaagtta cagtgatggc gatggcggcg
      721 caatcgatgt cacagataat aatagcgaca gcaagcatcc ttcaggttat acgataataa
      781 ataacactgc ctttacaaat aacactgccg aaggttatgg cggggcgata tataccaata
      841 gcgcgacggc tccctatctt attgatattt ctgttgatga cagctacagc cagaacggag
      901 gcgtgttagt cgatgagaac aatagcgcag caggctatgg agatggtcct tcctctgcgg
      961 cgggtggctt tatgtatctc ggcttaagtg aagttacctt tgatattgcc gacggaaaaa
     1021 cgctggttat tggcaataca gagaatgacg gagctgttga ctctattgct ggtaccgggt
     1081 taatcaccaa aacaggttcc ggcgatctgg tacttaatgc agataacaat gactttactg
     1141 gtgagatgca gattgaaaac ggtgaagtta ccctgggccg cagcaactcc ctgatgaatg
     1201 tcggcgatac gcattgccag gacgatccgc aagactgcta cggtctgacg atagggagta
     1261 ttgataagta ccagaatcag gcagagctaa atgttggctc cacccaacaa acctttgcgc
     1321 actcattgac gggctttcag aatggcactt taaatatcga tgctggtggc aatgttactg
     1381 ttaatcaagg cagttttgct ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa
     1441 acggcagcta tgtgctggcg ggggcgcagt cgatggcgct aaccggcgat atagtggtgg
     1501 atgctggtgc ggtgctttcg ctggaaggcg acgcggcaga tcttgccgct ctccaggacg
     1561 atccgcagtc gatcgtgtta aacggcggta tgctcgatct ctctgatttc tccacctggc
     1621 agagcggtac atcatacaaa gatggccttg aagtcagtgg cagcagcgga acggttatcg
     1681 gcagtcagga tgtggtagat cttgcaggcg gaaacgatat gcatatcggc ggcgacggga
     1741 aagatggcgt ctacgtggtg atcgatgcgg gtgacgggca ggtcagcctg gcaaatgaca
     1801 atcaatacct cggcacaacg caaatcgctt ccggtacgct gatggtgagc gacaactcgc
     1861 agcttggata tacccattat aaccgccagg ttatctttac cgataagcca caagaaagcg
     1921 tgatggagat tactgccaat gtcgatactc gctctacaac gactgagcat gggcgtgata
     1981 ttgaaatgcg cgccgacggt gaagtggcag ttgatgcggg ggtagacacg cagtggggcg
     2041 cactgatggc tgacagcagc gggcagcatc aggatgaggg tagcacattg actaaaacgg
     2101 gggcgggtac actggagctg accgccagcg gtacaacgca gtcagcggtg agagtagaag
     2161 agggcacgct gcaaggtgat gttgcggata tcttccctta tgcttcgtcg ctatgggtcg
     2221 gtgacggggc aacgttcgtt actggcgcgg atcaggatat tcagtcaatt gatgctactt
     2281 ccagcggcac tatcgacatc agcgatggta cggttttgcg cctgaccggg caggatactt
     2341 ccgtcgccct taatgcctca ctgtttaact gcgatgggac gctggtgaat gccaccgatg
     2401 gtgtgacgtt gacaggtgag cttaatacca accttgaaac tgacagcctg acttatcttt
     2461 ccaacgtgac ggttaatggc aatctgacca atacgtccgg tgcggttagc ctgcaaaatg
     2521 gcgtcgctgg cgatacgctg acggtaaacg gtgattatac cggcggcggt acgctactgc
     2581 tcgatagcga attaaacggc gatgactcgg taagcgatca attggtgatg aacggtaata
     2641 ctgctggcaa cacaactgtg gtggttaact ccattacagg gattggtgag ccgacatcga
     2701 caggcattaa agtggttgat ttcgcagctg atcccacgca gtttcaaaac aatgcgcagt
     2761 tcagtctggc aggcagcggc tacgtcaata tgggagcgta tgactacacg ctggtggaag
     2821 ataacaacga ctggtatctg cgatcgcaag aagtaacgcc gccatcgcca cctgatccag
     2881 acccgactcc cgatcctgat cccacgcagg atcctgatcc aacacccgac ccggaaccta
     2941 cgcctgctta ccagccggtg ttgaatgcca aagttggcgg ttatctcaat aacctgcggg
     3001 cggcaaatca ggcgtttatg atggagcgac gcgatcacgc aggtggcgat ggtcagacgc
     3061 tgaatttacg tgttatcggc ggagattatc attacacagc agcggggcaa ctggctcaac
     3121 atgaagacac ttctacggtg caacttagcg gcgatctgtt tagcgggcgc tggggcacgg
     3181 atggcgagtg gatgcttggg attgttggtg gctacagcga taaccagggc gacagccgct
     3241 cgagtatgac cggaactcgc gccgataacc agaaccacgg ttatgcggtt gggctgacct
     3301 caagctggtt tcagcacggt aagcagaagc agggggcctg gctggataac tggttgcagt
     3361 acgcgtggtt tagcaatgat gtttctgaac atgaagatgg cgtggatcat taccattcgt
     3421 cggggattat cgcctcgctg gaagcggggt atcagtggtt accggggcgt ggtgtggtga
     3481 ttgaaccgca ggcgcaggtg atttatcagg gcgtgcagca ggatgatttt accgccgcta
     3541 accgtgcgcg cgtgtcacaa tcgcagggtg atgatattca gacgcggctg ggtttacaca
     3601 gcgaatggcg taccgctgtt catgtcatac caacattaga tctgaattat tatcacgatc
     3661 cccattcgac ggaaattgaa gaagatgcca gcactatcag tgacgatgcg gtgaagcaac
     3721 ggggtgaaat aaaagtggga gtcacgggca atatcagtca gcgagtttcg ctgcgtggta
     3781 gcgtggcgtg gcagaaaggg agtgatgatt ttgcccagac ggcagggttt ttgtcgatga
     3841 cggtgaaatg gtaatcgctg tctgaccatc actattgctg gatgcggcgt aaacgcctaa
     3901 tcaggcctac ggagagcatt tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat
     3961 tggcgcggtt gccggatgaa tccaaacaac aacctcactt attctgcgtg tgcagcatat
     4021 agttcacatc cacgccgggg ccgagtttaa aagtattagt gatcgggttg taatgtagcc
     4081 cggttatatg tcgctcttta aggcttgtct gatctaccca gcccagcagt tctgccggtt
     4141 taataaactt cttcacatca tgtgtacctt taggcaccat gcgcaaaata tattccgcgc
     4201 cgactaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     4261 cgcccggttt caccagttgc gcacaggctc tgaccactga ctgcggatca gggacgtgct
     4321 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     4381 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     4441 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     4501 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataagccgc
     4561 cagcacgctc ggcaatatag ctcagacgca gcgggttaat gcggtgcagc ggtttgaact
     4621 caccttccag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     4681 ctacgttatg gtttaccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     4741 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaatc
     4801 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     4861 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     4921 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccaga tgtccgagat
     4981 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     5041 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     5101 catggtgact cggcagttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     5161 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     5221 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     5281 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc cgacgtcatg
     5341 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     5401 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     5461 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     5521 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgcggcaag
     5581 gtatatatcc gcgcccgtgc tgaagtggaa gttgacgcca aaaccggacg tgaaaccatt
     5641 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     5701 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     5761 ggtatgcgca tcgtgattga agtgaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     5821 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     5881 ggtcagccaa agatcatgaa cctgaaagac atcatcgcgg cgtttgttcg tcaccgccgt
     5941 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     6001 cttgaagcat tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     6061 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     6121 gttgccgcga tgctcgaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     6181 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcaattctg
     6241 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     6301 gagctgctgg atcagatcgc ggaactgctg cgtattcttg gtagcgccga tcgtctgatg
     6361 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     6421 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     6481 gtcgtgacgc tctctcacca gggctacgtt aaatatcagc cgctttctga atacgaagcg
     6541 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     6601 cgactgctgg tggcgaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     6661 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     6721 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc ggtgaccgag
     6781 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     6841 ctcaccgagt tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     6901 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     6961 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     7021 ggtgttcgcg gtattcgttt aggcgaaggt gataaagtcg tctctctgat cgtgcctcgt
     7081 ggcgatggcg caatcctcac cgcaacgcag aacggttatg gtaaacgtac cgcagtggcg
     7141 gaatacccga ctaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     7201 aacggtttag ttgttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     7261 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     7321 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     7381 gctgaaccgg ttgacgagga agatctggat accatcgacg gcagtgccgc ggaaggggac
     7441 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     7501 ttcatgccaa aagggagcca tctccctttt ttgaattgaa aagtccaggc tgcaaagtct
     7561 gggcttttgt cgtattaggg cgcggtaaag tttggctgtg tccgtaaaaa atggctggct
     7621 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     7681 cttgtaggtt ttggcgcaat agcactgctt tccgctggcg tctgggcgtt gcaatatgct
     7741 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaataattt gcaaattgat
     7801 ctcaatgagc cggacctctt tctcgacagc gattctctaa gccagcttcc caaagatctc
     7861 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     7921 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     7981 acgctgaaag ataagctctt ttcgtcactc ttagatcagc ccgcgcaggc ggcgctatgg
     8041 cacgataaac aaggccatct ttcacattac atggtgctga tccaacgcag tggtttaagc
     8101 aaactgctgg agccattgtt atttgccgct accagcgaca gccagttaag caaaacggaa
     8161 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     8221 aataacgccc tgatgttcgc gacatatcag gacaagatgc tggtgttttc cagcacggat
     8281 atgctgttta aagatgatca gcaggatacc gaagccacgg cgatcgcaag tgatttgttg
     8341 agcggcaaaa aacgctggca agccagcttt ggcctggaag agcgtgctgc cgaaaaaacg
     8401 ccagtacgcc agcgcatcgt ggccagcgcc aggttgctgg gttttggcta ccagcggtta
     8461 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     8521 gcgttaaatg atgaatccgc cagcgtagat gccagtttcg attttacgcc ggtctggaac
     8581 agtatgcctg ccggggccag cttctgtgtg gcggtgccgt attcacacgg tattgccgaa
     8641 gagatgcttt cgcacatcag ccaggaaaac gacaagttga atggggcgtt ggacggtgcc
     8701 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     8761 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     8821 cacgaaagca aagcgccaga aggtgttttg ccggtaagcc agactcagca gggcgaagcg
     8881 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     8941 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     9001 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     9061 acccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     9121 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     9181 gaaccggttt tttataacgc cgcacaaact ttattaatgc cgaagctgga cgctttatct
     9241 caacaaccgc gttatgtcat gaagctggcc cagatggaac ccggtgccgc ctggcagtgg
     9301 ctacccataa tctggctgcc gctatgaggc acgggctgct ggcgctgatt tgctggctgt
     9361 gttgtgttgt tgcccatagc gaaatgctga atgtcgaaca atccgggctg tttcgcgcct
     9421 ggtttgtacg cattgcacaa gaacagctcc gccaggggcc aagtccacgc tggtatcagc
     9481 aggattgtgc gggcctggtg cgatttgcgg cgaacgagac gctgaaagtt cacgacagta
     9541 aatggctaaa aagtaacggt ttatctagcc agtatttgcc gccagagatg acgctaacac
     9601 ctgaacagcg tcaactggcg caaaactgga atcaggggaa cgggaaaacc ggcccctacg
     9661 tgaccgcgat taatttgatt cagtacaaca gccagtttat tggccaggac ataaaccagg
     9721 cgctgcctgg cgatatgatt tttttcgatc agggcgatgc ccagcactta atggtctgga
     9781 tggggcgtta cgtcatctac cacaccggaa gcgccacgaa aactgacaac ggaatgcgcg
     9841 cagtcagtct gcaacaactt atgacatgga aggacacccg atggataccc aacgattcca
     9901 atcccaattt cattggcatt tatcgtttaa attttctggc gcgatagcag cgtgcttgtc
     9961 cctgtctctt gtgggaacag ggcttgctaa tgctgatgat tcgcttcctt ccagtaacta
    10021 tgcgccgccc gccgggggaa cattcttttt gcttgctgac agcagtttta gcagcagtga
    10081 agagtcgaaa gtgcgactgg aagcgccggg gcgtgattat cggcgctatc agatggaaga
    10141 gtacggcggc gtggacgttc gcctgtatcg tattcctgac ccgatggcat ttttgcgcca
    10201 gcagaaaaac ctgcatcgca ttgtggtgca accgcaatat ctgggcgacg ggctgaacaa
    10261 tacgctgacc tggctgtggg ataactggta cggcaaatct cgccgcgtga tgcagcgtac
    10321 tttctcttct cagtcacggc agaatgtgac tcaggcatta cccgaattac agctcggcaa
    10381 tgccattatt aaaccttccc gttatgtaca gaacaaccag ttttccccgc tgaaaaaata
    10441 tcccctggtg gaacagttcc gttatccact atggcaggct aaaccggtcg agccgcagca
    10501 aggggtaaaa ctggaaggcg catccagcaa tttcatctcg ccgcagccgg gtaacattta
    10561 tattcctctc ggccaacaag agccgggact gtacctcgtc gaggcgatgg ttggtgggta
    10621 tcgggcgacg acggtggtgt ttgtttccga taccgtggcg cttagcaaag tgtcaggcaa
    10681 agagcttctg gtgtggaccg cgggtaaaaa acagggtgaa gcgaagcccg gctcagagat
    10741 cttgtggact gacggtcttg gcgtgatgac ccgcggtgtg accgatgaca gcggtacctt
    10801 gcagttacaa catatatcgc cagaacgttc atacattctg ggtaaggatg ctgaaggcgg
    10861 cgtttttgtc tccgagaact tcttctacga aagcgaaatc tacaacaccc gcttgtatat
    10921 ttttaccgat cgcccgctat atcgcgcagg cgatcgtgtc gatgttaaag tgatgggccg
    10981 cgagttccac gatccgttgc attcatcccc catcgtcagc gccccggcga agctttcggt
    11041 gctggacgct aacggcagtc tgttgcaaac cgtcgatgtc acgctggatg cgcgcaatgg
    11101 cgggcaggga agtttccgcc tgccagaaaa tgccgttgcc ggaggttatg agttacgtct
    11161 tgcttaccgc aatcaggtct atagcagcag ttttcgcgtg gcaaactaca tcaagccaca
    11221 tttcgagatt ggtttagctc tcgacaaaaa agagttcaaa actggcgaag cggtcagcgg
    11281 caaactgcaa cttctctacc cggatggcga gccggtaaaa aatgcccgcg tgcagttaag
    11341 tttgcgcgct cagcaattat caatggtcgg taacgatttg cgttatgccg gacgtttccc
    11401 cgtgtcgctg gaaggcagcg aaacggtgtc cgacgccagc ggtcatgtgg cgttaaatct
    11461 ccccgccgcc gataaaccga gccgctattt gttaaccgtc tccgccagtg acggcgcggc
    11521 gtatcgcgtc accaccacca aagagatcct cattgaacgc ggcctggcgc attactcttt
    11581 aagtaccgcc gcacaataca gtaatagcgg cgagtcggtt gtgttccgtt atgccgcgct
    11641 ggaatcttca aaacaggttc ctgttacgta tgaatggttg cgtctcgaag accgcacgag
    11701 ccatagcgga gatctaccgt caggcggcaa atcctttacc gtcaatttcg ataaacctgg
    11761 caactacaat ctgacgttac gcgataaaga cggcttaatt ctcgccgggt taagccatgc
    11821 cgtcagcggt aagggcagta tgtcgcatac tggtacggta gatatcgtgg cagataaaac
    11881 gctgtaccag cctggcgaaa ccgcgaagat gctgattacc ttcccggagc caattgatga
    11941 agcattattg acgctggaac gcgatcgcgt tgaacagcag tcgctgctat cgcatccggc
    12001 aaactggctg acgttacaac gtttaaacga tactcaatat gaagcccgtg ttccagtgag
    12061 caattccttt gcgcctaaca tcactttttc ggtgctgtat acccgtaacg gtcagtacag
    12121 ttttcagaac gccgggatca aagttgccgt tccccagctg gatatccggg tgaaaacgga
    12181 caaaacccat taccagcctg gtgaactggt caatgtcgaa ttaacctcgt cgctgaaagg
    12241 taaacctgtt tctgcgcagc taacggtagg cgtggtcgat gaaatgatct acgcgctgca
    12301 accagaaatc gcgccgaata tcggcaaatt tttctatccg ctggggcgta acaatgtgcg
    12361 taccagctcc agtctgtcgt ttatcagcta cgaccaggca ctctccagcg agccggttgc
    12421 gcctggcgca actaaccgca gcgagcggcg agtaaaaatg cttgaacgtc cacggcgtga
    12481 agaggtggat accgcggcat ggatgccgtc actcacaacc gataaacaag gcaaagcgta
    12541 tttcacgttc ctgatgcctg attcgttaac ccgctggcgt atcaccgcgc gtgggatgaa
    12601 cggcgacggg ctggtcgggc aggggcgtgc ttatctgcgt tcggaaaaaa atctctacat
    12661 gaagtggagt atgccaacgg tgtatcgcgt gggcgacaaa ccgtcggcag gactgtttat
    12721 cttcagtcag caggataacg aaccggtggc gctggtgact aaatttgcag gcgctgagat
    12781 gcgccagacg ctgacgctgc acaaaggggc gaattatatt tcgctggcgc agaacattca
    12841 gcaatctggc ttgttaagcg cagaactgca acaaaatggg caagtgcagg acagcattag
    12901 cacaaaactg tcttttgtgg ataacagctg gcccgttgaa cagcagaaaa atgtcatgct
    12961 cggcggtggc gataacgcgc tgatgttgcc cgagcaggcg agcaatatcc ggctacaaag
    13021 tagtgaaacg ccgcaggaga ttttccgcaa caatcttgat gcgttagtcg atgaaccgtg
    13081 gggggggggt gatcaacacc ggtagccgtc tgatcccgct cagtctcgcc tggcgttcgc
    13141 ttgccgatca tcaaagtgcc gccgctaacg acattcgtca gatgattcag gataaccgtc
    13201 tgcggctgat gcaactggcg gggcccggag cgcgctttac ctggtggggt gaagatggca
    13261 atggtgacgc cttccttacg gcatgggcat ggtacgccga ctggcaggcc agccaggcgc
    13321 tcggcgtaac gcaacaaccg gaatactggc agcatatgct cgacagttat gccgagcagg
    13381 cagataacat gccgttattg catcgggcgc tggtgctggc gtgggcacag gagatgaatc
    13441 tgccgtgcaa aacgttgttg aaagggttgg atgaagctat cgcccggcgc ggaactaaaa
    13501 ctgaagattt ctctgaggaa gacacccgcg atatcaatga cagcctgatc ctcgatacac
    13561 cggaatctcc actggcagat gcggtggcaa acgtcttaac catgacgttg ctgaaaaaag
    13621 cgcagttgaa gtccacggtg atgccacagg ttcagcaata tgcgtgggat aaagcggtaa
    13681 acagcaatca gccgctggca cacacggttg tgctgctcaa tagcgggggc gacgctaccc
    13741 aggcggctgc tattttaagt ggtttgaccg ctgagcaatc tactattgag cgcgcgctgg
    13801 ccatgaactg gctggcgaaa tatatggcga caatgccttc ggttgtgttg cctgcgcctg
    13861 cgggcgcatg ggccaaacat aagttaactg gagggggcga atactggcgt tgggttggtc
    13921 agggcgtgcc ggacattctc tcttttggtg acgaattatc gccgcaaaat gtgcaggtcc
    13981 gctggcgtga agcggcaaaa acggctcaac aaagtaacat tccggtgacc gttgaacgcc
    14041 aattgtatcg gcttatccct ggtgaagaag agatgagctt tactctgcaa ccggtgacca
    14101 gcaatgagat tgacagcgat gcgctgtatc tcgatgaaat tacgcttacc agcgagcagg
    14161 atgcagttct gcgctatggt caggtggaag taccgctccc gccgggagcc gacgttgagc
    14221 gcacaacatg gggcatttca gtcaataaac ccaacgctgg aaaacagcag gggcaattgc
    14281 tggaaaaagc gcgaaatgaa atgggcgaac tggcctatat ggtgccggtg aaagaactga
    14341 cgggaacggt cactttccgc catttgctgc gcttctcgca aaaagggcaa ttcgttctgc
    14401 ctcctgctcg ttatgtgcgt tcctatgcac ctgcacagca aagtgttgcg gcagggagcg
    14461 aatggaccgg gatgcaggtg aaataagtga actggcgcag aatcgtctgg ctattggcgt
    14521 tggttactct gccaacgctt gcggaggaga cgccgttaca actggcgctg cgcggtgcgc
    14581 agcacgatca actgtatcag ctgtcgtcat caggcgtgac caaagtctca gcattacctg
    14641 acacgctcac cacgccgtta gggagtttat ggaaactgta tgtttacgcc tggctggagg
    14701 atacccatca accggagcag ccctaccagt gtcgcggaaa ctccccggaa gaggtttatt
    14761 gttgtcaggc tggggagagc ataacgcgcg ataccgcgct ggtacgttcc tgtgggctct
    14821 attttgcgcc gcagcggttg catattgggg cagatgtgtg ggggcaatac tggcaacagc
    14881 gtcaggcacc tgcgtggtta gcgtctcttt ctacgctgaa gccagaaacc tcggtaacgg
    14941 ttaaatcgct gcttgattca ttagcgacgt tacctgcgca aaacaaagct caggaagtct
    15001 tactggatgt ggtgctggac gaggcgaaaa tcggtgtcgc atctatgctc ggtagtcggg
    15061 tacgggtgaa aacctggagc tggtttgccg atgataaaca ggaaatccgt cagggtggtt
    15121 ttgctggctg gttaaccgat ggtacaccgc tgtgggtgac tggcagtggc accagcaaaa
    15181 cggtactgac ccgttatgcg acggtgctta atcgggtttt gccagtgccg acgcaagttg
    15241 ccagtgggca atgtgtagag gtggagctat ttgcgcgcta gccgctgaag aaaatcacag
    15301 cggagaagag tacgacggca gtaaaacccg gcgtacttaa tgggcgctat cgtgtgacgt
    15361 ttgccaatgg taaccacatt acgtttgtca gccacggtga gacgacctta ctgaccgaaa
    15421 agggcaaact gaaattgcag tcgcatctcg atcgtgaaga gtacgttgcc agagttctgg
    15481 atcgcgaggc gaaaagtacg ccacctgagg cagcgaaagc catgactgtg gcgatacgca
    15541 cttttttaca gcaaaacgcc aatcgggaag gcgattgcct gaccatcccc gacagcagcg
    15601 ccacgcagcg tgtttctgct tcgccagcta ccacgggggc gcgaacgatg accgtctgga
    15661 cgcaggatct catctatgcg ggcgatccgg ttcattatca cggcagtcgc gctaccgaag
    15721 ggacgctttc ctggcggcaa gccatggccc aggcggggaa gggagagcgt tacgaccaga
    15781 tactcgcgtt tgcttatccc gacaacagcc ttagccgctg gggagcgcca cgcacaacct
    15841 gtcagttatt acccaaagcg aaagcctggc tggcgaaaaa aatgccgcag tggcggcgta
    15901 tattacaagg cgagacgggg tacaacgaac cagacgtgtt tgcggtctgt cgtttagtct
    15961 caggtttccc ctataccgat cggcagcaga aacggctgtt tattcgtaac ttctttacgc
    16021 ttcaggatcg gctggattta acccatgagt atctgcacct ggcctttgat ggttatccca
    16081 ccgggcttga tgagaactat atcgaaacgc tgacccgaca attattaatg gactaatgct
    16141 atgcgaaaga tttttcttcc gttgttactg gtggcgcttt cgcctgtcgc tcacagtgaa
    16201 ggtgtgcagg aagtcgagat tgacgcaccg ctctctggct ggcatccggc agagggcgaa
    16261 gatgccagtt tttcacaatc gatcaattac ccggcgtcgt ccgtcaacat ggcagatgat
    16321 caaaatatct cagcgcagat ccgcggcaaa ataaagaatt atgctgcggc gggtaaagtt
    16381 cagcagggcc ggctggtggt caacggtgcc agtatgccgc agagaattga atccgatggt
    16441 tcatttgcac gcccttatat tttcactgaa ggcagcaaca gcgtgcaggt catcagcccg
    16501 gatgggcaaa gccgacaaaa aatgcagttt tactcaacgc cgggtacagg aacgattcgt
    16561 gcacgtttac ggctggttct ctcgtgggat acggacaata ccgacctcga tcttcacgtt
    16621 gtcacgcccg atggcgaaca cgcctggtac ggtaacaccg tgctgaaaaa cagtggtgca
    16681 ttggatatgg atgtcacgac ggggtacgga cccgagattt tcgccatgcc agcaccagtt
    16741 cacgggcgct atcaggtgta tatcaactac tatggtgggc gcagcgaaac ggaattaacc
    16801 accgcccaac tgacgctgat caccgatgaa ggatcggtca atgagaaaca ggaaacattt
    16861 attgtaccga tgcgtaatgc tggcgagctg acgctggtga aaagttttga ctggtgatcc
    16921 atcccttcac acaacctggc tctttctttt ttcagataag actgctgggc gatctgccga
    16981 cgccaggata taaccattaa caagcgtcgg cagattggtc atttcttatt tttcgtactg
    17041 agaactgtcc atttactccg gctttcctgg ttaaggcaat ggtttctaag cgatacgcat
    17101 tattgcaatg gtgacagaac attgcggtaa cacgctttta ccgctacctt aaccacactc
    17161 catcggtcac ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt ttcgtacaac
    17221 cctgaaagcc tcgcgctaca tgttcagagc attggcgtta gtgctctggc tgttgattgc
    17281 tttttcatcc gttttttaca tcgttaatgc gttacatcag cgagaatcgg aaattcgtca
    17341 ggaatttaat ctgagttccg atcaggctca gcgctttatt caacgcacct ctgatgtgat
    17401 gaaagagctg aagtacatcg ccgaaaatcg cttatcggca gaaaacggtg tgctttcccc
    17461 gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa ccgctgtttg ccgactccga
    17521 ttgttcagca atgagtaaca cctggcgagg ttctctggag tcattggcgt ggtttatgcg
    17581 ctactggcgc gataattttt ctgcggctta cgatctcaac cgggtatttt taatcggcag
    17641 cgataacctc tgcatggcca atttcggtct gcgtgatatg ccagtggaac gcgataccgc
    17701 gttgaaagct ttgcatgaac gcatcaataa atatcgaaat gcaccacaag atgatagcgg
    17761 cagtaacctc tactggatca gcgaaggtcc gcgccctggc gtcgggtatt tttacgcgtt
    17821 gacgccagtt tatctggcga accggttgca ggcgcttttg ggtgtcgagc agaccatccg
    17881 gatggagaac tttttcttac cgggtacgtt gccgatgggg gttaccattc ttgatgaaaa
    17941 tggtcatacc ctgatttcgc ttaccgggcc agaaagcaaa attaaggccg atcctcgctg
    18001 gatgcaggaa cgctcctggt ttggctatac ggaagggttc cgggagctgg tgctgaagaa
    18061 aaatctgcca ccctcatcgc tcagcatcgt gtattcggtg ccggttgata aggtgctgga
    18121 acgcattcgc atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg gagctgcatt
    18181 gtttactctc gcgcggatgt acgagcgacg tattttcatt ccggcggaaa gcgacgccct
    18241 gcgactggaa gaacatgagc agttcaatcg caagattgtc gcctccgcgc cagtgggtat
    18301 ctgcattttg cgtaccgctg atggcgtcaa tattttaagt aacgaactgg cgcataccta
    18361 tctcaatatg cttacgcatg aggaccgcca acgactgaca caaattatct gtgggcagca
    18421 ggtcaatttt gttgatgtcc tgaccagcaa caataccaat ctgcaaatca gcttcgtcca
    18481 ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg gtggatgttt cttcgcgcgt
    18541 gaagatggaa gagtcgttgc aggagatggc acaagcagcg gaacaggcga gccagtcaaa
    18601 atcgatgttc cttgccaccg tcagtcatga gctgcgaacg ccgctgtatg gcattatcgg
    18661 taacctggat ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcgtc tggtgacggc
    18721 aatgaacaac tcttccagcc tgttgttgaa aattatcagc gatattctcg atttctcgaa
    18781 gattgaatcg gaacagttga tgatcgaacc gcgtgagttt tcaccgcgtg aagtgatgaa
    18841 ccacatcacc gccaactatt taccgctggt ggtacgcaag cagttaggct tgtactgctt
    18901 tattgaaccg gatgtgccag tggccttaaa tggcgacccg atgcgtttac agcaggtcat
    18961 ctccaacctg ttgagtaacg ccataaaatt caccgatacc ggctgtatag ttttgcatgt
    19021 ccgcgctgat ggcgattatc tctctatccg tgttcgcgat accggcgtgg ggattccggc
    19081 gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc ggaacgggcg tacagcgaaa
    19141 cttccagggg accggtctgg gtctggcgat ttgtgaaaaa ctgatcagca tgatggacgg
    19201 cgatatctcg gtagattcag aaccgggaat gggcagccag tttaccgtgc gtattccgtt
    19261 gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg ttgagtggta aacgctgctg
    19321 gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa accagtttgc agcgcagcgg
    19381 catcgtcgta acaacatacg aagggcagga accgactccc gaagatgtgt taatcactga
    19441 cgaggtagtg agtaaaaaat ggcagggcag agcggtagtg accttctgtc gtcgacatat
    19501 tggtattccg ctggagaaag cgccagggga gtgggtacac agtgtggctg ctccgcatga
    19561 gctaccggca ttgttggcgc gtatttattt gatcgagatg gagagcgacg atcctgctaa
    19621 cgctctgccg tcgacggaca aagcggtcag cgataatgac gatatgatga ttctggtcgt
    19681 ggatgatcat ccgattaacc ggcgtttgct ggcagatcag ttgggatcgt tgggctatca
    19741 atgtaaaacc gcgaatgatg gcgtcgatgc gcttaatgta cttagcaaga atcatattga
    19801 tatcgtgctt agcgacgtca acatgccaaa tatggatggt taccgcttga cacaacgcat
    19861 tcgtcagttg ggactgacgt tgccggtaat cggagtaacg gctaatgcgt tggctgaaga
    19921 gaagcagcgg tgtctggagt ccggtatgga cagctgcctg tcgaagccag taacgctgga
    19981 tgttataaaa cagacgctga cggtatatgc cgagagggtc aggaaatcgc gggaatcgta
    20041 ggacggataa ggcgtttacg ccgcatctgg cattcagtgc aaatgccaga tgcgacgctg
    20101 acgcgtctta tctggcctac tttaatgtaa tctgttgaaa taatgggaat cgtaggacgg
    20161 ataaggcgtt tacgccgcat ccggcaatcg gtgcaaatgc cagataagac actaacgcgt
    20221 cttatctggc ctacagatga ttagtcttta tctgccggac ttaacgttac tgaagagaga
    20281 taattcagca gggcgatatc gttctcgaca cccagcttca tcatcgcaga tttcttctgg
    20341 ctactgatgg ttttaatact gcggttcagc tttttagcga tctcggtcac caggaagcct
    20401 tccgcaaaca ggcgcagaac ttcactctct tttggcgaga gacgtttgtc accgtaacct
    20461 ccagcactga ttttttccaa caggcgagaa acgctttccg gggtaaattt ctttcctttt
    20521 tgcagcgctg cgagggcttt cggcagatcg gtcggtgcac cttgtttcag cacgatccct
    20581 tcgatatcca gatccaatac cgcactaaga atcgccgggt tgttgttcat ggtcagaaca
    20641 ataatcgaca ggcttgggaa atggcgcttg atgtacttaa ttaaggtaat gccatcgccg
    20701 tacttatcgc caggcatgga gagatcggta atcaacacat gcgcatccag tttcggcagg
    20761 ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa cattcaccca ctcaatttgc
    20821 tcaagtgatt tgcgaatacc gaacaagact atcggatggt catcggcaat aattacgttc
    20881 atattgttca tgtaataggc taccttgcta cagcaagctc ttgacataac tgtcaatgtc
    20941 gctgatgtat ttttctattc ctggaacatc cttctcacga atcagatgtt ccagcgtttc
    21001 acataactgc ttgccgggta ccagatttag catggcaaat acgcctttaa gacgatgagc
    21061 cgtttgggct aacgcagcaa agtcactggt tgctgcttca gtatacagcc tcttaacatc
    21121 atccggtact gtgtctacaa agagcgcata atagccgctg gcatggagtt gcgcattttc
    21181 atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt caattaattg
    21241 taagaccgct tcctgcatag cgttgctcat attgaagttg acgcacaatt gaccaggccc
    21301 aatttcccgt acgccagact catcatcgct taaaagcaag ccagaggcag taagattaga
    21361 cggattatcc gttaaaaaga tatcataatc ttgactaatt aatctttcat cgggtgtgat
    21421 acaggttgca ccccaatttt ctaactggcg agtgacaata ttccgaattt ctgccgaagt
    21481 aacatccacc attacgcaga catcatccag taaacgctct tcttcctctt caacttccgg
    21541 gtcagctgcg agcattttga tatgcacaga gtagcgtgta ccaagcccat cccgcgtttt
    21601 gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg ctcagccaga atgccagcgg
    21661 gtccgccttg ccatagcgat cgttttgggt ctggttgata aacgggaagt gcaaattatc
    21721 catttcatga atgcttacgc cttctccggt gtccagaatg cggaacgtca ggcggtcttc
    21781 ggaggactca tcctgatcaa cctcaagggt gatttttccc aattgcgttg aggtcacggc
    21841 atattgcatc agtagcagca aaatacgtcg taatgcatcg cgatcgccgc ggcgcatatc
    21901 gtgtgctttc agatgattgt taatcagcag ttgcagacct ttacgcttga tggcaggcaa
    21961 cactgaaggc acaacttcat caattaaatc ctgcacggag aacagcaccg tctcactttt
    22021 ccagctatcg tccgcaagca tgttcgctaa ctgtatttca tcgaccaatc gcaccagcac
    22081 atctgcctga ttcgccagtt gtttgctttc cggggcgttg agtttagccg cgctctccgc
    22141 cagggactgt gcgggttctt tcagcgcatc gccaatgttt ttcataaagg tcatccgccc
    22201 ctgctggttt ttctcataca gacgctgcgc ctgcttgagt ttcttgttta ccagcacttc
    22261 gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc gcaacctggc tgcggaacat
    22321 gcggatctca tacagctcgt tattgatcgt cgcctgaata atcccctgat gctgttccgc
    22381 catggtggtg atgttttgca gattcaaatg cggcagcaaa tgatcggcaa ttttgttact
    22441 tatgacagtg cggttcgatt cctgatcgtg aaccagcagg ccgagcggca gcagtgagac
    22501 tatctcttca ttgattgccc gtaaaatgcg caattcgtta ttgaccgccg tgttgggtag
    22561 actttctgta ctgcgactgg agaaatggcg gaatgtggta tagccaaata acgccagcgc
    22621 cagcaaaccg atgttcagca gcagtggcag cagaatgttt tgcaacgtat ccagcaataa
    22681 ggtgccataa ggaacctgcc agaccaggcg catatcggta gagttgagtg ccgaggagat
    22741 ttcaatcttc gtactgttaa agtggatact gacgctatcc gttccttctt tctcattatc
    22801 attgtttccc gttgccgtcg cgtctggctc aaggcggaaa ctgtccagcg gcatacccgg
    22861 tgggatcaaa tcattaatcg gcagatcaaa agccacgacc gttgccagat gtcccggctg
    22921 gttgaacgtg gtacgcaagg taaagtaatg accgttctgc caggccaggc ggcgcatgtt
    22981 agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc aacatctctg cacgacgtga
    23041 atcaacaatg tcactgacgg tcgattcttt aaatccggag gtgagatctt tgaggggcag
    23101 ggttgagatc agcaccagac tgttatcctg accattcagg taatacattg accacggtac
    23161 attttctgcg ccccacaatg tgtccagata ggtggacatc cgttgagtca tctcaagcgt
    23221 tgagttgtcg tgagagccaa agattaacgc ttccgttttg cggcgcggtt tttccagata
    23281 gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg ccggaggagg gggaggtcgt
    23341 cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga cgcgcttttg
    23401 gatagcgtgg gtaatgtcga caatttggta acttttatct ttcagccagg cgttaacggc
    23461 gctttgtacc attacaccca tcgtcaccag taacacaatg atcaacagta aaaagaagcg
    23521 ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt tctgacgcat
    23581 tcgtgtttat gacctgttaa aacttcgcga agtgataggg cagtataaag ggtacagtgt
    23641 gaaattccag actcttacct gattgatgat tctctattct cccgtgatat ttatcatcct
    23701 gcattggggg aaatagatgt acaagcgcca ttttgctgta caaataacct acaaaaagcc
    23761 caacgaaaat aattgcaaat aaacaaagat tgctggaaat tatgctgatg ttaattattt
    23821 gtgaaatagt taacaagcgt tatagttttt ctgtggtagc acagaataat gaaaagtgtg
    23881 taaagaaggg taaaaaaaac cggatgcgag gcatccggtt gaaatagggg taaacagaca
    23941 ttcagaaatg aatgacggta ataaataaag ttaatgatga tagcggtagt tattctagtt
    24001 gcgagtgaag gttttgtttt gacattcagt gctgtcaaat acttaaga
//
LOCUS       DAZFDC010000068        24020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFDC010000068 DAZFDC010000000
VERSION     DAZFDC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.068"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(702..1133)
                     /locus_tag="ACHJ48_002788"
     CDS             complement(702..1133)
                     /locus_tag="ACHJ48_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO4708310.1"
                     /translation="MVLRSIWVEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(1201..3222)
                     /locus_tag="ACHJ48_002789"
                     /pseudo
     CDS             complement(1201..3222)
                     /locus_tag="ACHJ48_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3356..3481)
                     /locus_tag="ACHJ48_002790"
     CDS             complement(3356..3481)
                     /locus_tag="ACHJ48_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO4708311.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(3423..3572)
                     /locus_tag="ACHJ48_002791"
                     /pseudo
     CDS             complement(3423..3572)
                     /locus_tag="ACHJ48_002791"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(3746..4507)
                     /locus_tag="ACHJ48_002792"
     CDS             complement(3746..4507)
                     /locus_tag="ACHJ48_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO4708312.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRNQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFCVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAAKE
                     AGHAVREMERWMVPNKLREVRDAATITRIARKQPE"
     gene            complement(4504..4938)
                     /gene="psiB"
                     /locus_tag="ACHJ48_002793"
     CDS             complement(4504..4938)
                     /gene="psiB"
                     /locus_tag="ACHJ48_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO4708313.1"
                     /translation="MKTELTLNVLHTMNAQEYEDIRAAGSDARRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(4993..6951)
                     /locus_tag="ACHJ48_002794"
     CDS             complement(4993..6951)
                     /locus_tag="ACHJ48_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO4708314.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADTFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADNDYRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQSD
                     KSLALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPDGWARDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(7017..7250)
                     /locus_tag="ACHJ48_002795"
     CDS             complement(7017..7250)
                     /locus_tag="ACHJ48_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO4708315.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIENDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGIIK"
     gene            complement(7307..7759)
                     /locus_tag="ACHJ48_002796"
     CDS             complement(7307..7759)
                     /locus_tag="ACHJ48_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4708316.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLVRAAGAQTQPEEGQQFSGQPQPQPPEGDDYGFSDDIPF"
     gene            complement(7785..7991)
                     /locus_tag="ACHJ48_002797"
     CDS             complement(7785..7991)
                     /locus_tag="ACHJ48_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4708317.1"
                     /translation="MHIPQNFLPKSRQKVLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            8061..8588
                     /locus_tag="ACHJ48_002798"
     CDS             8061..8588
                     /locus_tag="ACHJ48_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708318.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GCATGTVAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRRKRKR
                     KKKKNSNRHGNHRKITSEKNIPDTSQTGRVTDDASAWPASLPAGVSLPGVFARADRRP
                     PPPALYHLFLLPCKM"
     gene            complement(8558..8749)
                     /locus_tag="ACHJ48_002799"
     CDS             complement(8558..8749)
                     /locus_tag="ACHJ48_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708319.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(8746..9168)
                     /locus_tag="ACHJ48_002800"
     CDS             complement(8746..9168)
                     /locus_tag="ACHJ48_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4708320.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(9215..9640)
                     /locus_tag="ACHJ48_002801"
     CDS             complement(9215..9640)
                     /locus_tag="ACHJ48_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4708321.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADACGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(10058..10834)
                     /locus_tag="ACHJ48_002802"
     CDS             complement(10058..10834)
                     /locus_tag="ACHJ48_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708322.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
     gene            complement(10880..11434)
                     /locus_tag="ACHJ48_002803"
     CDS             complement(10880..11434)
                     /locus_tag="ACHJ48_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO4708323.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALKIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(11328..11549)
                     /locus_tag="ACHJ48_002804"
     CDS             complement(11328..11549)
                     /locus_tag="ACHJ48_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708324.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(11550..12233)
                     /locus_tag="ACHJ48_002805"
     CDS             complement(11550..12233)
                     /locus_tag="ACHJ48_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO4708325.1"
                     /translation="MSRFIQGDCVRVMATIPGNGVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(12310..12615)
                     /locus_tag="ACHJ48_002806"
     CDS             complement(12310..12615)
                     /locus_tag="ACHJ48_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708326.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(12619..13521)
                     /locus_tag="ACHJ48_002807"
     CDS             complement(12619..13521)
                     /locus_tag="ACHJ48_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO4708327.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGRRRWEELPDDARESITALFTPKRGDWCDIWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLSTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAVLSRRFSCMLEHWYAGQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            complement(14216..15187)
                     /locus_tag="ACHJ48_002808"
     CDS             complement(14216..15187)
                     /locus_tag="ACHJ48_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFO4708328.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(15187..16353)
                     /gene="sopA"
                     /locus_tag="ACHJ48_002809"
     CDS             complement(15187..16353)
                     /gene="sopA"
                     /locus_tag="ACHJ48_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFO4708329.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(16941..17186)
                     /locus_tag="ACHJ48_002810"
                     /pseudo
     CDS             complement(16941..17186)
                     /locus_tag="ACHJ48_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
     gene            complement(17866..18672)
                     /locus_tag="ACHJ48_002811"
     CDS             complement(17866..18672)
                     /locus_tag="ACHJ48_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO4708330.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPNLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(18673..18978)
                     /gene="ccdB"
                     /locus_tag="ACHJ48_002812"
     CDS             complement(18673..18978)
                     /gene="ccdB"
                     /locus_tag="ACHJ48_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO4708331.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(18980..19198)
                     /gene="ccdA"
                     /locus_tag="ACHJ48_002813"
     CDS             complement(18980..19198)
                     /gene="ccdA"
                     /locus_tag="ACHJ48_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO4708332.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            19741..20340
                     /locus_tag="ACHJ48_002814"
     CDS             19741..20340
                     /locus_tag="ACHJ48_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4708333.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            20337..20957
                     /locus_tag="ACHJ48_002815"
     CDS             20337..20957
                     /locus_tag="ACHJ48_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708334.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRWKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            20954..21154
                     /locus_tag="ACHJ48_002816"
     CDS             20954..21154
                     /locus_tag="ACHJ48_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708335.1"
                     /translation="MKIYHYTDLNGLKGIIESGSLWATHFSFLNDSNELTHGMNCLEN
                     ALQYLRDDFNPKTLKFIEQAPL"
     gene            21562..22539
                     /locus_tag="ACHJ48_002817"
     CDS             21562..22539
                     /locus_tag="ACHJ48_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO4708336.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PVSLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(22824..23564)
                     /locus_tag="ACHJ48_002818"
     CDS             complement(22824..23564)
                     /locus_tag="ACHJ48_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4708337.1"
                     /translation="MNNVIPLQNSPERVFLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(23685..23915)
                     /locus_tag="ACHJ48_002819"
     CDS             complement(23685..23915)
                     /locus_tag="ACHJ48_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO4708338.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWHYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
BASE COUNT         5707 a         6835 c         5984 g         5494 t
ORIGIN      
        1 tatttacgta cggatgaagg ctggccgtat ctggctgtgg tcacgtgcca ttattggctg
       61 atcaatgtcg tcacgcatga cggcacagga gcccccggaa cgttatgcta ccgcatgtca
      121 tttgcaaaaa tgacagatag atatacttca atttgaatct taacaattag catgggaatg
      181 ttgtcaatcc tctgtatgga gccaccacat attgcaattt ttgttctctt ttaaaatata
      241 ttccacgagc gtcttgtggg accttttgct gttatagggg ggcagtttgg atatcgaaaa
      301 ttattgtacg ttaaggctga atttcaatcg caaagcataa ccttaccttt ctgcttactt
      361 atgcagttca aagagtacca actctgcaat tttcgttgcc acagatgcaa tatcgttact
      421 ttccagtcac catgccgctg aaagcccaca ccatgcgata atccacatca agagccgctt
      481 tctgtccaga cccgctgttg tgaggtgtga ggtgtgaggt gtgaggtgtg aggtgtgagg
      541 tagcctgagt ttaacggaca ggccgatgaa ggacgccgtt accgggataa tgtaagaatc
      601 tttttttgag tatgtcaaca taacctgagg caaagtacct caggttccag taatcagccg
      661 acctgacagt cttctgttag ccctacagat atgtcgtgta gctaaccgcg catgtcaaag
      721 ccggattttt gaaaatctct ataaacttcc ggattgttga aggccgggaa tttagcttta
      781 tgggctgctg actttatcgc ttcgcaatag gctttatcgc cattactggt tgatattttt
      841 aacgccgtgc catcccggga gaattccata tgcaacctgc attttttcct tttccatttt
      901 tgcggatcat caagtttagc attaattgca tccctgattc cccgtgcctg ctccccccat
      961 tcatcctgat catcccaacg tcctgaactg caactaccca gggctgtagt tttatggcaa
     1021 tttgaaggat gtaatggcgc acatcccgcc acaaagctga ctaaaatagc cagtataacg
     1081 attttcttta cccccatata ttgctcctca acccatatag atctcagtac catagctctg
     1141 tcagagcaaa gaaaatctta actaatacaa gttaagattt ttaatatcca ctcctcatcc
     1201 ctacattcca ggtgatggtg gcgtccactc attatcctga actggagcat aagatgcgcc
     1261 atgccccccg gatatgcttt tctgttgcct gatatattcg tggtagtact gttcactacg
     1321 ctgacgatgt aaatccacat ccgggcggct gattacaaag tgtccatttc cggttattcc
     1381 agctgcatta tgccaggcca ccatttccgc aaatacatca ttaaactgtt gatatgagcc
     1441 ttccggttca aaacccattc ctggctcacg atcaaacaat acaggagcaa tcagattcag
     1501 cttatccacc agttcatttc tgtcctgatg atggtagaac accacacggt ccccataatc
     1561 cacacagact aactcgccat tcctccgatc acgaaaacgc acactgccat ctttctgcag
     1621 acgggcttcg agaacgccag tgtcttcata aagtggagtt cctcccggtt cacagagaat
     1681 cacgcagcgg tcggcattcg ttgttcgtcg cttgtctttg cgacgatcac gataatccca
     1741 gccacgtaac tgtgataatg cagctctgtc tccctgtacc gcctgttgct ccacccactg
     1801 ccggtaggac aaaggatacc acttaccctc ctcaatcagt gacaaccgct cttccttcac
     1861 actctccttc agcctgatca gcgcctgcat acgctgcact tccgcaatat ggtaatgcag
     1921 cttacgcaat tgtggatccc ggaactgcac acggatatac gccttgcgcc gacggcaggc
     1981 agcatgaatt tcccgtaacc gttctccgta acgtaagtcg ggtttacgcc agtgctcctt
     2041 ccatgcaaga taacgcgcgc gcaaatcttc ccgcgcagct gcacgggctt cacggcgttc
     2101 acgacgcagt acaggatcgc gccggaagcc gggctccact tcatcgctgg cagcaagttc
     2161 cggattgtag cgacattcag gcttcacccg ttcaaatata tctgcacctg caatctcaaa
     2221 tggccctgcc tgcggctcag ccctggataa cgtcaaatcc gggtggatac tgctggcctt
     2281 cactggtgtg agctcatgat taaacgcatc cacaatcaca agaccatgat gctgtttctg
     2341 caacataagc cctttacgcg caaacatctc atggcacgcc tgccagctga aattcccgcc
     2401 cgcgatcata tcttcaatca acgactcaca ctgaacagtt gtgtaccccc acagactttc
     2461 cttatcagaa aacagttcgc gctttattgc tccctgtgga cctgtcccca tccgcatacg
     2521 ataccattcg taatcactga cagcctgagt accaagatta cgtccccgga accgttctcc
     2581 gggtataacc tgagtaaaaa tatccttcgg tacaggctgc catccaccat ccagatcctg
     2641 catataatcc agcgtcatta acggccacac actgtctgca cgtactggtg tctgttgttt
     2701 atcaaatcca tcatgcagta ccagatgccc gtgctggatc cccatccata atccagccgt
     2761 tgccagagtt ctgtgcacat ccaggcaact ttccagctca ccatttctcg ccatttccgg
     2821 taagcgttcg gcaaaatgac ggcaggcgta ctgtttcaga ctctccgttt ctgccacttt
     2881 ttccggccgg atatcaacgt ttatggcttc cggatcataa cgaccgggtg tccccacctg
     2941 actgaaaata tctgtcggta caggctgata ttcccccagt ttccgcccca gtttttcagc
     3001 tgtccataac ggtccgaacg aactgagtgc aacaccttcc cgcgctctgt cccagccatc
     3061 cttcaccacc agttcccctt cctgcatcgt gatatacaga ccatcactgg ccagccgttt
     3121 atgcaaagat aaccagtcct gtgcgggctc ttcacgcagc ccggcaatcg acatctgagc
     3181 aatatattcc ctgaacgtct gctttttccc gcgtcgccag gctgttcatg cccgtacaat
     3241 gccagcaagg agaaaggggc caccggcgaa ccagcagccc ctttataaag gcgcttcagt
     3301 gcagtcagac cagcatcagc cctgaaaagg cgggcctgcg cccgcctcca ggttgctact
     3361 taccggattc gtaagccatg aaagccgcca cctccctgta tccgtctctg taacgaatct
     3421 cgcacagcga ttttcgtgtc agataagtga atatcaacag tgtgagacac actatcaaca
     3481 cacaccagac aagagagctg cgtggtagtt tcatggcctt cttctccttg cgcaaagcgc
     3541 ggtaagaggc tatcctgatg ttgtctaagc atgcaggggc ctcgtgggtt aatgaaaaat
     3601 taactacggg gcttttgtcc ttctgccaca caacacggta acaaaccacc ttcacgtcat
     3661 gaggcagaaa gcctcaagcg ccgggcacat catagcccat ataccagcac gctgaccaca
     3721 ttcacttttc ctgaaaataa cccgctcatt caggctgttt acgggcaatc cgtgtgattg
     3781 ttgccgcatc acgcacctcc cggagtttgt tcggcaccat ccaccgctcc atctcacgga
     3841 cagcatgacc ggcctccttt gctgccagac tggcttccgc gataacctgc cagaatggca
     3901 tatcctgcca ctgccagcgt tccattccgg ccagtgacgg aaaacgctcg ccccactgcc
     3961 agaacagggt ttccaggtca tgctctgcca cccgatcact ccagcgtgca taccagcttc
     4021 cgactgtttc cggtgaatgg aacgccagtt cgatttcagc ctgggcgacc agcgcctggt
     4081 acgcacgggc tttctgctct gcctccctcg cctgctggcg gctgtacttc tgcatgacca
     4141 gcgcggattt atggcgactg cgttccctga cacaaaaccg caccgacggg aacaagcgtt
     4201 ctgctgcatc accgggaagg ggaaggaggc agacctcgcc ctgtgtttca atcagcacat
     4261 cgatggcata caaccactga agacggtttc cgtgcagttc ttctgcggtc agggacggca
     4321 tgaaaaatcg caggtcagac agcgatatcc tgcggcttcc gttcagacag cggaaaaacg
     4381 cccccgcata cggatattcc gccagcgtgt ttccctgatt gcggcgtttt tccgtctctg
     4441 ccactgcccg ccatgcggcc tgttgctctg tgctgagggg aaccagtgcc tgtgaacgtg
     4501 cgctcatacc tgacctcctt ccgccatcag gatatggatg atgtcgttaa cgctgtatcc
     4561 ctgcgtatcc agtgacgccg ccagtgccag tgaatggctg acggcttccg gtgcaaaccg
     4621 ccgctgtacc tgaaccaccg caaacggctc accaccggca ttcaccagaa ccagcaccca
     4681 gacctgggag acatcgcccg gtgaacataa cgccaggtga aaacgttcgt gggcaggcgt
     4741 gaaacggacc tggacgggga aaaagccgcc aaactcgctg ccgtactcgc cgttcatcgt
     4801 ccagttgtcc ggcgcatcca gctcccgcat cacggcgtgt gtcagctcac ggcgtgcatc
     4861 acttccggca gcccggatat cttcatattc ctgtgcgttc atggtatgta atacattcag
     4921 agtcagttca gttttcatca cggtatctcc cttgctgccg ccccgtctcc ggcggggcgg
     4981 tgtggttgtt catcaggcgg catcagccag gttatcggtg cgttcagtgg catccgtctg
     5041 tgggcgtggc gcacacatcc agccaggcac ccagcggttg tctttcatat ggtgctctgc
     5101 atgttcagcc gcatcgcctt tcttcatctt ctccgcgtcc cgtgcggcac cggatagtcc
     5161 tgcctcattc agggctgcga taatctgcgg ctttttcagg tgtccgaaaa agtttgcttt
     5221 cgtcggctgc caccagtcgc gcatgtgaaa gccgatggcg ctctccagcg tgtccagcgg
     5281 actgcgtgac gtgtgaccat actcacgggt ctggacaccg tggatactgc acgcggtgca
     5341 gaaactgagc agtgataaca gcacctcctg actgagtgac aggaacgtcg tcatatcccg
     5401 tgcccatcca tccggcagca gggcggcaag acgggctttt tctgccatca tcgccatgaa
     5461 tgcggcacct tccttccctg acggcgcatc gctggtcagc gaataatgct cacattccag
     5521 gcggattctg gcgggattgc tgtacgctcc actgccaaat acattcagac agagtgtcca
     5581 tgccagaagt gccagggatt tgtctgattg ctgcatcagt gccgcctgga ctgccagcgt
     5641 gcgctctgag gacattttcg tcagcagcgg cagactgatt tcctccactg aggctttctc
     5701 ctgcacctgt tccgtgcggt aatcgttatc agccacatca tcctcactgc gcaactgcac
     5761 accacgctgg acacacacgt tgccataacg ccaggacacc accacgccac ttccggcacg
     5821 catctccggc gtccacgccc tgaccttcgc catgcagcgc atcagcttca tttcggcttc
     5881 cagcaggtcg gattcctcac actggttttc cagcgcatcg taacgtgcca tcagttcgtt
     5941 caggcgttct tcttctgcct ccgtcagcac cgcttccggc tccggcagac tgcggtatgt
     6001 tccggcatcc tcacggcaga aaccaacagg ctccatacgt ccggcgcacc attcccagcc
     6061 ttcggcttcc cgaaggtgtt cggcgacagc ctggagtttt tccagcaggg cggcatcgag
     6121 cgccacgcag tccacatagc caccctcgtc gtcactgaac aaatcggtgc gcagttcatc
     6181 tggcgagaag gtatcagccc ccacgaagcg gaatttactg ttccctgcca ccgccacttc
     6241 actttcggtc accagacgac gaatggtctg tacttccggt ttaccgcccc atcccgactg
     6301 acaggcggct tcaaacacct gcacctgacg cgcggtgtcg ttctccagcg ccagcgcctg
     6361 acagtgctcg gtggtgatgc ggtcttctgc cagcgcatcg aggatgacag gcgcaaggtc
     6421 tgccagtttc agcattcgct gaacgtggcg gggcgaataa cccagcaaat caccgatttg
     6481 tgcaggtgtt ttgccttcct gcgccattgc acggaatccg gcaatctgtt cggcagggtg
     6541 catatcccga cgatgaccgt tctcggtcat cgatgcggca gtcgccagct cctgcggaat
     6601 gaccttcaca cggacaggcc agtcagccgg aatgatgtca cgctctgcca gcatgttgag
     6661 tgctgccagt cggcgaccac ctgcggcgac accgtaacgg tcacctggca gggcatgaac
     6721 aaccagattc tgtaacaggc cgacaccttt aatggagtcc gccagttcgc tgacggactc
     6781 cgcagaatac ggcaccgtgc gcacattcag cggtgactta atcagcgagg ccagcggcac
     6841 gctcacttcc tccgtctgcg ccagcagggc cgacaggact gtttcctgcg tttttgcagg
     6901 tttacggctg gatttacgct ccgtttttgc cttagactct gtaactgaca tagttagtac
     6961 ctcccaaaac ggtaataatg attgtctgcc ggacgcgttc ccgtccggca cctctcttac
     7021 ttaataatcc cgaaatcacg gatgacatgg ttcatccagt acccggcacc gtcctcaaaa
     7081 ttccaggtac gccagaccat tgcaccgtta ttacggacaa ccaggcgaaa ctgttcgccg
     7141 tggtcattct cgataaagac gttccggtac tgtgcggcaa cggcttcggc atgtttgcgg
     7201 gtaaaaggac cgtcaggcag tccctgaagt attctgaaat attcgctcat cctcatctct
     7261 ccggtgatgc cccgcacagg acggggcggt tgtcacagtc agtcgttcag aacgggatat
     7321 cgtctgaaaa cccgtaatcg tcaccctccg gcggttgcgg ctgaggctga ccgctgaact
     7381 gttgcccctc ttccggctga gtctgagcac ctgcggcacg taccagcatc tgcatggtgc
     7441 ccgtggtctt aacaagaatt tcggtgacgt aacgggtgat accgttatcc tcccagctac
     7501 gggtgcgaag ctgaccttcg atgtagacct gcgcgccctt gcgcagatat tcacctgcca
     7561 cttccgcgag cttgccgaac agcaccacgc gatgccattc tgtctgctcc cgcatctccc
     7621 ccgtctgttt gtcacgccag ctttctgacg tggccacctg caggtttgcc actgcgcccc
     7681 cgttggggat gtaacggact tccggatctt ttcccaggcg tcctacgagg atgaccttgt
     7741 taatgccacg aactgccatg atgtgtctcc ttctgttgat gttttcagcc cccatcatct
     7801 tttgagtttc gggagccgcg gttttgacgt tttgcgggga cctgcctttt gttccctccg
     7861 gtattcacca tcaccttttt gagaaccttc gccgcaaggg gcatcactgc cacggccgga
     7921 aacgactgtc cgggggcaga ccgcaaaact ttttgcctgc ttttgggcaa aaagttttgc
     7981 gggatgtgca tttcacccct ggactggcgg agacggttgt ggctgtcttt gcccccggcg
     8041 acaggggctt aaacaggtga atggcaccgg acacaacaac gggcaggcac ccgccgcaac
     8101 gacgtgaagc gcggtttgcg aaacggcgtt gccccttaca tcccggcggc cttttgccgg
     8161 gatgccggga gcgcaggcca cggcgcgaca gaggctgcgc gaccggaacg gtcgctgcaa
     8221 gccagggttt cgcgtcaggg atggaagccc gccagggcgg agacagcttc gctggctccg
     8281 ttcacgacag cccgccccgc cagggggacg cccggaaaag ccataagcag gagaggaacg
     8341 gaaagaagag gaggaagagg aagaggaaaa agaagaagaa cagtaacagg cacggtaacc
     8401 acagaaaaat aacatcagaa aaaaacattc cggacacatc acaaacagga agggtaacgg
     8461 acgacgcgtc agcgtggccc gcttcccttc cggcaggcgt cagcctgcca ggggttttcg
     8521 cgcgggctga caggcggcct ccaccgccag ccctgtatca cctgtttctg ctgccctgta
     8581 agatgtagtg accgcccagg aaaacagcct gctgattcgc cacaaaatcg cagatttcat
     8641 caacaatctg cgcggtggtg agcggtttct gccccatttc acgacgtcgc gcattaatca
     8701 cggctttgta attgcgcaga atttcgcggg tttctgtgct gatattcatg acatgtgctc
     8761 cccgtccagc gcctgtaaca caacgtccat tgcttcgcgt tcctccctga cgaacgcatc
     8821 accgtcgccc ccgttctcac tcccgacggc atacaggcgc ttcatttcac tgcctgccag
     8881 cgccatcact ttttccagca tcaccgccgg aactgtcacc tgacggctgg cacgcttttc
     8941 ttccatcaac tcacgaacaa cgtcgtgaac aatgctgaca tccgcaccat attcaaaggc
     9001 atcgtccagc cgctgcatga cggtttccag ctcatcgtct gacaggttcc agtcctgggc
     9061 tatgtggcgc acatccccac gggtcagcac cacggcaaaa acgcattcac tgtccggcac
     9121 gtctgtatcc agtacatcgc ggatgatttc tttaacagta cagtacatgt tctcgtctcc
     9181 tttacagggg cggcattgcc gccccgtccg ttaatcagtc cagtgccgcg taaatcgcgt
     9241 tgcattcagg gtgaaattcg atgtgacgcc acaactgcgc atcgcgcatc cggtaaagct
     9301 gggtcagtcc tgcatcaccg ctgtcgtggt acagccacaa ctggcggtta atcacaaggg
     9361 aagtcagaat gatgccgcag gcatcagcac tcacggtacg gtcaaaccag tttgcaccgt
     9421 tcaccatgtg gaaacggtcg ccatccggca tcatgtaacc gccaccgtcc ggcaggcgga
     9481 tgaagtgcca gaagcctccc tgatattccg gcatcatttt ttgcgccagc gcgtacacat
     9541 acgcctcggc atacagaaaa tcgttgccaa acaggtaagg cagaaaggag agacggtcgc
     9601 actcttcaac attcagagtg acaggtttcg catattgcat gatgttttcc tccgttgttc
     9661 acatattccg cgacaccggg cggtgccgtg cctccagaaa agccgggcga taaggcgggg
     9721 atgcacaggg cgcaagggaa gccgcagcct gacgcacacg gctgacggga gttgcagggt
     9781 gaggccgcga aacctgagcg ggcgaacgac cggaaaatca cggcagacgg gaaggaggga
     9841 ccgggtgacc gcagcggcga agcgtaccct tgcatcaacg ccagcccggc taagatggag
     9901 gcacggcacc gcccgcacac gacgctgagg ccacataact gaaaaaccac aaggccatgc
     9961 cctgcatggc cgggtaaagc ggagccagcg gcgacttcct ttgtgctctg tccggggacc
    10021 ggcaggaaaa aggcggggat ttaccccgcc aacgtcatca tttccggatt tttgccaggg
    10081 catccgccat gctgcggatt tcgttccggg cttcccggac cacctcccgc agaacaccac
    10141 ggtcaaacca ccggcggggc ggttcatccg gtatacgcag cacatcgcag gccagcccac
    10201 tggacaggat atgttcaata caatcgctga ccagatacat ttccaccgcc gaaaaacgtg
    10261 cgccccggtt caggatggca tcgagcacca cgggcaggtt ttcgtcgtcc tcatcgagcc
    10321 agcaggggtg atcgctttcc ttcacgacca ggcgaaagcg gcgcgtctgg cgggtcagta
    10381 acgtctgacc ggtggtggtc agcgccgccc ggatacgccg cgtatcttct gccacccggc
    10441 aactgacact gcgtaccgca tcgcgcaccg ccgcaagtac agccctgatt tcgtcttcgg
    10501 tcagccattc accggcatac ggaaaagaca cctgcgttcc gtctgccgga agcaccggca
    10561 gcgactgcca ctgactgccg gacagaacaa gttccgctcc ggttccggtc agtttctgaa
    10621 tggcggaggc caggtgcggc ggcagtacgc cgccgtggcg gttaacgagt aacgtcgcca
    10681 gccggggcgc atccagcaga acataaatgc tgatccagcc cggatacttc gggttacgct
    10741 ggtcgacatc cgtctcttcc ggccagccct gcgccagcag caggttacgg gcgtgacgac
    10801 aaatcagtgc gttcagcgtt gtgttttcgt tcatgattaa tcgttctccc tgtatcgccg
    10861 cccgcaggcg gcgataacat cagccgtttt cgtgtgtgtt gtttttcagc aggatgcggg
    10921 cctgattaac caccgcctga cggcgggtaa cgcattccgg gacggcgaga ccgtaatcac
    10981 gggcggccca ttcacgtttc cacagtgcct gttcggcggt ctgaatcagg gacgccagca
    11041 gttcagccgg gacggtcacc tggcggtttt cgctcacgtt gctgataatc tccatcgccg
    11101 cggcagaaga aataccggac tcaattcgct ggtcttccgg gatgtcctcc aggcgcgcca
    11161 ggacggtcct gatttcagga tcggacaggg aaatatccat tccgtcagcg agggcctgaa
    11221 tctcttccgg ggaccagata accagcatca gcggtgtata tcccggatac tttgctgcca
    11281 gctcccggca tagcgtttcg catgttccgt acattctcgt ctccttctca atctttaaag
    11341 cacgcatcca gttccagaat ttcggtatac gcctccatga acaggcgatt ggcgcgatac
    11401 agcgtctggc gtgccaggcg ttcaacaagc acatcggaat caaagcgaca gcgatgctcc
    11461 atatcgttca gcctggcccg aaggtcacac atggtgttgg ccacctccgc cacgtcggcg
    11521 cgaagttttt catgtccggc atagttcatt tacgcagcct ccggcataaa ccagtcatca
    11581 tttgcggccc cctgctgcat ggcccgctgt acggcagcca gacgttgctg tccggcacgg
    11641 tgatactgtt caagcagctc gataccgata taccggcgtc cggactgaag ggcggccaca
    11701 caggttgagc cgctgcctgc aaacgggtcc agcacaatcg cgttcgggtg tgtgaagctc
    11761 tcaatcagtg gttgcaggct ggtaacaggc ttttccgtcg ggtgatgacg attgcctgaa
    11821 tatttccagc ccagcacgtc gggcaatggc ttctgaggca gcgccggacg cccttttgcc
    11881 aggatgtaag cgcattcgtg gcgatagccc acatatgcgg ccttcgaagt gtaggttttg
    11941 gtaaacacca ggtggccaac cacgctgaat cccgcatttt tccaggcggc cataaagcga
    12001 tcgacgcggt tccagccata gaagctcacc atcagcgcat cttttttcag cacgcggtac
    12061 atttcattac aggccggttg cagccattca tcggttttat cgccggcgat ggtacgcccc
    12121 tgacggtcac ggaaaccgac gagatatggc gggtcggtga ggatgaaatc gacaccattg
    12181 cccggaatcg tggccataac acgcacgcag tcaccctgga taaaacggga cataacagca
    12241 ctccttctct tacagagggc accgggaggg cgcccctgtg gcgcccgttc cccgcggcct
    12301 gtgggctgtt caccgcgtcc ggtaacgggt caggccgacc gtggaatatc gcagccgtcg
    12361 tctttttgac ggcgagaata tgccgcgaaa gcggcttgaa gggcgttcat cagaaaaggg
    12421 cgacgggctg gcgtacactg tcaccgatgc cagccgggag cggtaaaagc gcccgtcccg
    12481 tgcccggccg gacgcgggac actgaccaca ggcagcgttc ctgccgcgcc gcgtcagccg
    12541 gcgcagtaaa aagaggagcc cggcgacatc ctttaccgga aaagaaacgg gcggttgccc
    12601 gcccgtctcc gtcatacttc atccaccgta atgcgccacc ttgtcgacta tccactccgg
    12661 cgcggtgact tccggcagct catcatcatc tgtcggggaa gaccattcca gttcccccca
    12721 cagcacatca acaagctctc cacgctcata tcgctgccag ccacagaaat tgcagccctg
    12781 cccggcgtac cagtgctcca gcatgcaact gaaacggcgg cttaatactg caataacgtc
    12841 gctttccggc tgacaccacg gcgtgtcgaa atcaacctga atgaagttgt ctccctgact
    12901 gctgatattc acctcatacc cacaaggcca cttcacgcca tactgttccg tataccagtg
    12961 atatgcagaa ggaacaccgt tcagaacacc accgttaaag ccattcactt caacatccag
    13021 gcgagtggac agaaccgtca gcaggtcaaa cggcatggtt ttttccggca ggacgttgtc
    13081 acacagacgg ttccaccaca cagatacatc ctcgttactc cagatgtcgc accagtcacc
    13141 tctttttggg gtgaaaagag cggtaatgct ttccctggca tcatccggta attcttccca
    13201 gcgacgccgg ccagtaccgc tctgcagcca gagttcatgc agcatcaggc agttttgctc
    13261 atccagaagt acaccgtcct gcaggtgtgt cagccagcgg gtgaacgcga tattctccgg
    13321 cgatacaacg ccacgcccgg cagccgtcag tccggggcat ggttcaaacc gcacatcttc
    13381 agtggtctgc agaagtccgg cacttccggc cagaaacagc tgaatacctt catttgtggc
    13441 gcggcgataa agcggtgtga ctgcaccgct ggccagtcgt ttaatctcag cgatctgtgc
    13501 cggttcacca gaaaaataca tacgattcga gcaccagttt ggcatgggaa ttctcctctc
    13561 acatgcggcc tccatcaggc cgtgccgcac ctgtaaccgg gcgaccgcat acgaatgcag
    13621 gtgcggcccc gaagccgcag cgcgtggagg aggaacgacg aaacgtgacc gggtgaggag
    13681 gccggcgaca gcgacacact tgcatcggat gcagcccggt taacgtgccg gcacggcctg
    13741 ggtaaccagg tattttgtcc acataaccgt gcgcaaaatg ttgtggataa gcaggacaca
    13801 gcagcaatcc acagcaggca tacaaccgca caccgaggtt actccgttct acaggttacg
    13861 acgacatgtc aatacttgcc cttgacaggc attgatagaa tcgtagtctc acgctgatag
    13921 tctgatcgac aatacaagtg ggaccgtggt cccagaccga taatcagacc gacaacacga
    13981 gtgggaccgt ggtcccagac cgataatcag accgacaaca cgagtgggac cgtggtccca
    14041 gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagtctgat tatcagaccg
    14101 acgatacaag tggaacagtg ggcccagaga gaatattcag gccagttatg ctttctggcc
    14161 tgtaacaaag gacattaagt aaagacagat aaacgtagac taaaacgtgg tcgcatcagg
    14221 gtgctggctt ttcaagttcc ttaagaatgg cctcaatttt ctctatacac tcagttggaa
    14281 cacgagacct gtccaggtta agcaccattt tatcgccctt atacaatact gtcgctccag
    14341 gagcaaactg atgtcgtgag cttaaactag ttcttgatgc agatgacgtt ttaagcacag
    14401 aagttaaaag agtgataact tcttcagctt caaatatcac cccagctttt ttctgctcat
    14461 gaaggttaga tgcctgctgc ttaagtaatt cctctttatc tgtaaaggct ttttgaagtg
    14521 catcacctga ccgggcagat agttcaccgg ggtgagaaaa aagagcaaca actgatttag
    14581 gcaatttggc tgtgttgata cagcgggtaa taatcttacg tgaaatattt tccgcatcag
    14641 ccagcgcaga aatatttcca gcaaattcat tctgcaatcg gcttgcataa cgctgaccac
    14701 gttcataagc acttgttggg cgataatcgt tacccaatct ggataatgca gccatctgct
    14761 catcatccag ctcgccaacc agaacacgat aatcactttc ggtaagtgca gcagctttac
    14821 gacggcgact cccatcggca atttctatga caccagatac tcttcgaccg aacgccggtg
    14881 tctgttgacc agtcagtaga aaagaaggga tgagatcatc cagtgcgtcc tcagtaagca
    14941 gctcctggtc acgttcatta cctgaccata cccgagaggt cttctcaaca ctatcacccc
    15001 ggagcacttc aagagtaaac ttcacatccc gaccacatac aggcaaagta atggcattac
    15061 cgcgagccat tactcctacg cgcgcaatta acgaatccac catcggggca gctggtgtcg
    15121 ataacgaagt atcttcaacc ggttgagtat tgagcgtatg ttttggaata acaggcgcac
    15181 gcttcattat ctaatctccc agcgtggttt aatcagacga tcgaaaattt cattgcagac
    15241 aggttcccaa atagaaagag catttctcca ggcaccagtt gaagagcgtt gatcaatggc
    15301 ctgttcaaaa acagttctca tccggatctg acctttacca acttcatccg tttcacgtac
    15361 aacatttttt agaaccatgc ttccccaggc atcccgaatt tgctcctcca tccacgggga
    15421 ctgagagcca ttactattgc tgtatttggt aagcaaaata cgtacatcag gctcgaaccc
    15481 tttaagatca acgttcttga gcagatcacg aagcatatcg aaaaactgca gtgcggaggt
    15541 gtagtcaaac aactcagcag gagtgggaac aatcagcaca tcagcagcac atacgacatt
    15601 aatcgtgccg atacccaggt taggcgcgct gtcaataact atgacatcat agtcatgagc
    15661 aacagtttca atggccagtc ggagcatcag gtgtggatcg gtgggcagtt taccttcatc
    15721 aaatttgccc attaactcag tttcaatacg gtgcagagcc agacaggaag gaataatgtc
    15781 aagccccggc cagcaagtgg gctttattgc ataagtgaca tcgtcctttt ccccaagata
    15841 gaaaggcagg agagtgtctt ctgcatgaat atgaagatct ggtacccatc cgtgatacat
    15901 tgaggctgtt ccctgggggt cgttaccttc cacgagcaaa acacgtagcc ccttcagagc
    15961 cagatcctga gcaagatgaa cagaaactga ggttttgtaa acgccacctt tatgggcagc
    16021 aaccccgatc accggtggaa atacgtcttc agcacgtcgc aatcgcgtac caaacacatc
    16081 acgcatatga ttaatttgtt caattgtata accaacacgt tgctcaaccc gtcctcgaat
    16141 ttccatatcc gggtgcggta gtcgccctgc tttctcggca tctctgatag cctgagaaga
    16201 aaccccaact aaatccgctg cttcacctat tctccagcgc cgggttattt tcctcgcttc
    16261 cgggctgtca tcattaaact gtgcaatggc gatagccttc gtcatttcat gaccagcgtt
    16321 tatgcactgg ttaagtgttt ccatgagttt cattctgaac atcctttaat cattgctttg
    16381 cattttttta ttaaatcttg caatttactg caaagcaaca acaaaatcgc aaagtcatca
    16441 aaaaaccgca aagttgttta aaataagagc aacactacaa aaggagataa gaagagcaca
    16501 tacctcagtc acttattatc actagcgctc gccgcagccg tgtaaccgag catagcgagc
    16561 gaactggcga ggaagcaaag aagaactgtt ctgtcagata gctcttacgc tcagcgcaag
    16621 aagaaatatc caccgtggga aaaactccag gtagaggtac acacgcggat agccaattca
    16681 gagtaataaa ctgtgataat caaccctcat caatgatgac gaactaaccc ccgatatcag
    16741 gtcacatgac gaagggaaag agaaggaaat caactgtgac aaactgccct caaatttggc
    16801 ttccttaaaa attacagttc aaaaagtatg agaaaatcca tgcaggctga aggaaacagc
    16861 aaaactgtga caaattaccc tcagtaggtc agaacaaatg tgacgaacca ccctcaaatc
    16921 tgtgacagat aaccctcaga ctatcctgtc gtcatggaag tgatatcgcg gaaggaaaat
    16981 acgatatgag tcgtctggcg gcctttcttt ttctcaatgt atgagaggcg cattggagtt
    17041 ctgctgttga tctcattaac acagacctgc aggaagcggc ggcggaagtc aggcatacgc
    17101 tggtaacttt gaggcagctg gtaacgctct atgatccagt caattttcag agagacgatg
    17161 cctgagccat ccggcttacg gtactgtgac aattttttag attgtcacac taaataaaaa
    17221 agagtcaata agcagggata actttgtgaa aaaacagctt cttctgaggg caatttgtca
    17281 caacaccttc tctagaacca gcatggataa aggcctacaa ggcgctctaa aaaagaagat
    17341 ctaaaaacta taaaaaaata attataaaaa tatccccgtg gataagtgga taaccccaag
    17401 ggaagttttt tcaggcatcg tgtgtaagca gaatatataa gtgctgttcc ctggtgcttc
    17461 ctcgctcact cgagggcttc gccctgtcgc tcgactgcgg cgagcactac tggctgtaaa
    17521 aggacagacc aaacatcatg gttctgtgtt cattaggttg ttctgtccat tgctgacata
    17581 atccgctcca cttcaacgta acaccgcacg aagatttcta ttgttcctga aggcatattc
    17641 aaatcgtttt cgttaccgct tgcaggcatc atgacagaac actacttcct ataaacgcta
    17701 cacaggctcc tgagattaat aatgcggatc tctacgataa tgggagattt tcccgactgt
    17761 ctcgttcgct tctcagtgga taacagccag cttctctgtt taacagacaa aaacagcata
    17821 tccactcagt tccacatttc catataaagg ccaaagcatt tattctcagg ataattgttt
    17881 cagcatcgca accgcatcag actccggcat cgcaaactgc acccggtgcc gggcagccac
    17941 atccagcgca aaaaccttcg tgtagacttc ctttgaactg atggacttat gtcccatcag
    18001 gctttgcaga actttcagcg gtataccagc atacagcatg tgcatcgcat aggaatggcg
    18061 gaacgtatgt ggtgtgaccg gaacagagaa cgtcacaccg tcagcagcag cggcggcaac
    18121 cgcctcccca atccaggtcc tgaccgttct gtccgtcact tcccagatcc gcgctttctc
    18181 tgtccttcct gtgcgacggt tacgccgctc catgggtatt ttcagtgttg ccaccatcgt
    18241 ctgcagctgg ctgacgtacc aggagtcaga gagcggaacc agccggtgag tctgctgacc
    18301 ggcgggcatt ctccccgccg tcctggcagc tttttcggtc cgttgtttca gggtcgcaag
    18361 ctgcacaaac ggatacggag gcgcaagcga aaaatccccc cgcgtcagcg ccagtgcttc
    18421 attaatgcgt gctccggtgt tccacagtgt ggccagcagc atcttgcggt gcagattcgg
    18481 gacgtaatgg agcagggcac tcacttccgg agccagcaga tattttggca gttcatcatg
    18541 gaccatcgac atctggcgaa gtgccagagc tgccggataa tcaatggcaa caggcagcga
    18601 tgcaggctgt ccggcagaat acactgccgg taccatgact gcagactggc tgtgtataac
    18661 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc
    18721 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc
    18781 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga
    18841 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt
    18901 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt
    18961 gtaaacctta aactgcattt caccagtccc tgttctcgtc agcaaaagag ccgttcattt
    19021 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac
    19081 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat
    19141 atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag
    19201 cacacctctt tttgacatac ttcgggtata catatcagta tatattctta tgccgcaaaa
    19261 atcagcgcgc aaatacgcat actattatct ggcttttagt aagccttatg tattttacct
    19321 ttcgttatgt taaccaataa aaattaaaat ctgccttata aaaacaaagc gtaattaccg
    19381 cattcccgtt tcgtatggat ccgactttat ttgaataaac gactttccgg aagtgtacca
    19441 cctgagaaaa tcatcatatc gctgttttcc gcccctgcgc ggcgcatcac agtccagaga
    19501 tacaacacaa catatagtgc catgggcaac tgaatgaggc actgcatgct gtgctgacgg
    19561 gatgtaacgc tgtgcggagg gagaggaaga tccgtaacca ccagaaaccg gatctgacca
    19621 ggaaaagaga aagagtgtat gtaagttctg tactgtatgt atgaattgta ttgtatgtat
    19681 tgtgtatttt tcataccggg catacgatgg cgccattccc tgagaaaccg gagccagacc
    19741 atgacatccc tctccccgga caccgtccgc cgcattgaag acgccgccgc cgccctgatc
    19801 gccgccggca ccccgaaccc gaccaacgag caggtccgtc agcacctcgg cggcggctcc
    19861 ctgtcccaca tctccccggt gatgcgcgcg ttccgcgccc gccagcgcga gcaggccacg
    19921 cccctgccgc cggagctggc gcagctgctg accggccagc tggggctgct gtggcaggcg
    19981 gccgtgaaac aggctgaagc cggggccctg gcggcgcgcg agcaggccga cgacgacatt
    20041 gcccgggccg acaaggagcg ggatgaggcg ctggcgaacg tggccgcgct ggaaagcgag
    20101 ctggcggtgc tgcgcgaggt ggtggccgaa cgtgaccgcc tgctgcagga ggtacgcgag
    20161 ctacgcgcgg aggcgctgcc gctgcgcgag caggtggccc gcctgaccgc caccggcgaa
    20221 cacctggcgg cgcagctgca ggacacgaag gcggagctga aggaagcccg ggaggacggc
    20281 cgccagcttc agaccgaact gctggctctg gcccgccagg acgggaaggt gaagaaatga
    20341 gacaggaaag agtattacgt ataaaggcat ttcgacgggc actggagctt ggtggcggtg
    20401 aacgggctga cgggtgtcgt cattttcccc gctggaaaac ggaactgagg aatttcccgc
    20461 acagcagctg cgatttagcc agcaatactc tggcgtacta cctcaaagaa cgagagagat
    20521 gccatccgtg catcattttt atggaaggga acgctggctt tcatgaagcg gaaaactcaa
    20581 cagttcacgg gcatgtcatc gtattgctgg acggagagta catcgatttg acgctggatc
    20641 aattcgacga atatccggac tacattcctt acgaacctgt cgaatctgac gggcagattg
    20701 gtaagctgtt gcgtaatatc atgaagcatg aggacccggt aaaaacccgc cagattgatc
    20761 tcgatggtgg cgaagagctg tatgcctggt tacgcgatac cgccgacgaa gtgctggcag
    20821 cagatccgga atggcaggcc cgggaacggt cgatagaaga agcaagggaa gccgctatta
    20881 cggtgttccc gtttctgtct gacgtgcaaa aaactgagtg cgaacagggc ccggacagtc
    20941 aggaggctgc cagatgaaga tttatcatta cactgacctg aacggcctga agggcatcat
    21001 cgaatcaggc tcactgtggg cgacgcactt ttcctttctc aatgacagta atgagctgac
    21061 tcacggcatg aactgccttg agaatgccct gcagtacctg agggacgact ttaacccgaa
    21121 aacccttaag ttcatcgaac aggcaccctt ataagcggtt gtgtatgctg tttccctgga
    21181 atgatttatc cacatatccg gaggctcgat ccagtaaaca gatccatgaa tgatcaacaa
    21241 aggatccatt aaagatcccc ataccgctgc aaaccttgtc actcatgggc cgggaccacg
    21301 atcacataag cagtggcatg ttactgataa actgtaacat gctaatgata agctgtattc
    21361 agtaatccat atactgaagt aagttaatga cataaactat ggtcagtacg ccagactcag
    21421 ctgttaaata caggctgcag gtttttcttc agtcagttag cggggctctg acacacgatt
    21481 tgctgtttat tcttttactg tccacaggca ggaggctttc tggaaaacga aaattcagac
    21541 atcaaaaaac tgttcggcga ggtggataag tcgtccggtg agctggtgac actgacacca
    21601 aacaataaca acaccgtaca acctgtggcg ctgatgcgtc tgggcgtttt tgtaccgacc
    21661 cttaaatcac tgaagaacag taaaaaaaat acactgtcac gtactgatgc cacggaagag
    21721 ctgacacgtc tttccctggc ccgtgctgag ggattcgata aggttgagat caccggcccc
    21781 cgcctggata tggataacga tttcaagacc tgggtgggga tcattcattc ctttgcccgc
    21841 cataacgtaa ttggtgacaa agttgaactg ccttttgtcg agtttgcaaa actgtgtggt
    21901 ataccttcaa gccagtcatc acgcaggctg cgtgagcgca tcagcccttc cctgaaacgc
    21961 attgccggta ccgtgatctc cttttcccgc accgatgaga agcacacccg ggaatacatc
    22021 acccatctgg tacagtcagc ctactacgat actgaacggg atattgttca gttacaggct
    22081 gatccccgcc tttttgaact gtaccagttt gacagaaagg tccttctcca gcttaaggcg
    22141 attaatgccc tgaagcgacg ggagtccgcc caggcactct acacctttat agagagcctg
    22201 ccccgggatc cggcaccggt atcgctggcg cggctgcgtg cacgcctcaa tctgaagtct
    22261 cctgtatttt cccagaacca gacggtcaga cgggcaatgg agcagctgcg cgagattgga
    22321 tatcttgatt acacggagat ccagcggggg cggacaaaac tcttctgcat tcactaccgg
    22381 cgtccccggt taaaagcacc gaatgatgag agtaaggaaa atccgttgcc accttcacct
    22441 gcggaaaaag tcagtccgga gatggcggag aagcttgccc tgcttgagaa actgggcatc
    22501 acgctggatg acctggaaaa actcttcaaa tcccgctgaa cataaactgt agtcagtgaa
    22561 gagtgttcct ttactgacta cagcttatat tatcaggtgc agtgagtggt ctgctcactg
    22621 cagtttatat tcagtttcct gcagtgctgc ctgtagctga gctgtcatct gccggtccct
    22681 tacgtgagtc accccgtaac ctgatgctga ggcattgctc ccttcataaa acatgactta
    22741 ctcactacag cttatataca tgctccagct tatgttatgt ctgttctgct gaccacagct
    22801 tatataagga agcgcgtatg ccgtcacctc aggggaggca gtgtacgcag gatctccgca
    22861 gcatcccgcc cgtcacctgt gaaaggcact gccagcgtgg cagccatatc cagcgcaaac
    22921 actctggtat aaacctccat cgaacgtgga tccctgtgac ctgccagggc ctggatgact
    22981 ttccggggct ggcggtgata gagcatatgc atgatatagc tgtgccggaa ggtgtgtggt
    23041 gtgaccggaa tcgaaaagtg tactccgtca gcttcggccc gtctgacagc ctgcttcagc
    23101 cagttgcgca tggtttcgtc ggtcacggcc cataatggtt cacgacgacg gggccgggtg
    23161 gtgatcatcc agctttccat ctgcctgaca tagcttatat ccgtcagcgg aaccaggcgg
    23221 acttcatctt ttggcgggcg tccgcgtcgc gcacgcactt tttcggacag gatccgcaca
    23281 aacggccttg ctccatccag gtcaaatgat tccggcgtca gcatccgggc ttcgccaata
    23341 cgcattccgg tattccagag ggtggcgaac agcatatggt gacgctgatc cggcatatag
    23401 aaaagaaggg cactcacttc cggggccagc aggtaggccg gtgtggcccc tgtggaagtg
    23461 gccattcttc tcagggagag cgctgttgca aaatccaccc ccggcgcaat gggtaacagg
    23521 aagacgcgtt ctggtgaatt ctgcagggga atgacattgt tcatgagtta tttttcacgt
    23581 agcacacgca ctgtttatgg atacaggtat tctgacctga acgcccggat ccttccacga
    23641 caataaatcg cagcgtgcgg atccttttgt ttgcttatca cgcattattg cgggtaaaac
    23701 caaatacgta aaggtacgtt ttgcagcagg gatcccgtat taagccagtc gtggcgcggg
    23761 ctgagggctg gtttgccgtt tttgggagaa ttggggtaat gccaggacgc taatgatcta
    23821 tcaatggatg gatgtaagtg acaattgaaa actgaaaaat gcgctatgta gattctgaca
    23881 gatttatgca cctgaaatac gttcaaaatc cacatagcgt aactctgatt ttccttacca
    23941 gaaaaaagag ggggaataac cagtatctat cccccctaca caattgcaga ataataaatt
    24001 ataaaaataa gaactcagga
//
LOCUS       DAZFDC010000069        23713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFDC010000069 DAZFDC010000000
VERSION     DAZFDC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.069"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(196..1479)
                     /gene="gltA"
                     /locus_tag="ACHJ48_002820"
     CDS             complement(196..1479)
                     /gene="gltA"
                     /locus_tag="ACHJ48_002820"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO4708339.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(1685..1792)
                     /locus_tag="ACHJ48_002821"
     CDS             complement(1685..1792)
                     /locus_tag="ACHJ48_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708340.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2173..2577
                     /gene="sdhC"
                     /locus_tag="ACHJ48_002822"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2173..2577
                     /gene="sdhC"
                     /locus_tag="ACHJ48_002822"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4708341.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2571..2918
                     /gene="sdhD"
                     /locus_tag="ACHJ48_002823"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2571..2918
                     /gene="sdhD"
                     /locus_tag="ACHJ48_002823"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO4708342.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFCASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2918..4684
                     /gene="sdhA"
                     /locus_tag="ACHJ48_002824"
     CDS             2918..4684
                     /gene="sdhA"
                     /locus_tag="ACHJ48_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO4708343.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4700..5416
                     /gene="sdhB"
                     /locus_tag="ACHJ48_002825"
     CDS             4700..5416
                     /gene="sdhB"
                     /locus_tag="ACHJ48_002825"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO4708344.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5717..8518
                     /gene="sucA"
                     /locus_tag="ACHJ48_002826"
     CDS             5717..8518
                     /gene="sucA"
                     /locus_tag="ACHJ48_002826"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO4708345.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8533..9750
                     /gene="odhB"
                     /locus_tag="ACHJ48_002827"
     CDS             8533..9750
                     /gene="odhB"
                     /locus_tag="ACHJ48_002827"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO4708346.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9844..11010
                     /gene="sucC"
                     /locus_tag="ACHJ48_002828"
     CDS             9844..11010
                     /gene="sucC"
                     /locus_tag="ACHJ48_002828"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO4708347.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11010..11879
                     /gene="sucD"
                     /locus_tag="ACHJ48_002829"
     CDS             11010..11879
                     /gene="sucD"
                     /locus_tag="ACHJ48_002829"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO4708348.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            12546..13451
                     /locus_tag="ACHJ48_002830"
     CDS             12546..13451
                     /locus_tag="ACHJ48_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4708349.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDTGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFEEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(13485..14087)
                     /gene="cobO"
                     /locus_tag="ACHJ48_002831"
     CDS             complement(13485..14087)
                     /gene="cobO"
                     /locus_tag="ACHJ48_002831"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO4708350.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(14097..15749)
                     /locus_tag="ACHJ48_002832"
     CDS             complement(14097..15749)
                     /locus_tag="ACHJ48_002832"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4708351.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDQQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(15857..17137)
                     /locus_tag="ACHJ48_002833"
     CDS             complement(15857..17137)
                     /locus_tag="ACHJ48_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFO4708352.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVAAEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(17298..17615)
                     /locus_tag="ACHJ48_002834"
     CDS             complement(17298..17615)
                     /locus_tag="ACHJ48_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFO4708353.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(17612..18982)
                     /locus_tag="ACHJ48_002835"
     CDS             complement(17612..18982)
                     /locus_tag="ACHJ48_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFO4708354.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKERAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(18986..20227)
                     /locus_tag="ACHJ48_002836"
     CDS             complement(18986..20227)
                     /locus_tag="ACHJ48_002836"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFO4708355.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(20227..21672)
                     /locus_tag="ACHJ48_002837"
     CDS             complement(20227..21672)
                     /locus_tag="ACHJ48_002837"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFO4708356.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(21691..23079)
                     /gene="glmL"
                     /locus_tag="ACHJ48_002838"
     CDS             complement(21691..23079)
                     /gene="glmL"
                     /locus_tag="ACHJ48_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFO4708357.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTPHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPVAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(23079..23528)
                     /gene="glmS"
                     /locus_tag="ACHJ48_002839"
     CDS             complement(23079..23528)
                     /gene="glmS"
                     /locus_tag="ACHJ48_002839"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFO4708358.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         5688 a         5964 c         6223 g         5838 t
ORIGIN      
        1 atcgattggc gggataacgg tggtggtgaa tgaaaaatta ggattttatt tagttatcgg
       61 atatgtattc ttgtaacgtg taattatgta tttgtgtccg attacgtttt tttgtcgata
      121 gacataacta acgaacaaaa aaaagcccgc cagatattca ggcgggctaa acgatatatg
      181 gttcaaatca gataattaat gtttaacatc tgatttaaag tctcgttttt catagccggt
      241 gtacaactgg cgcggacggg cgatcttcat gccgtcactg tgcatttcgc tccagtgagc
      301 gatccagcca acggtacggg ccatagcgaa gatgacggtg aacatggaag acggaatacc
      361 catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt tcttctcgat
      421 aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca gcaggtcatc
      481 tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg tggcgcgcgg
      541 gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat catttttatc
      601 tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt ccagcatttt
      661 cagcgccgct tcgttagcac caccgtgtgc aggcccccac agtgaagcaa tacctgctgc
      721 gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg aggtagaggc
      781 gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt ccagaatcgg
      841 attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt taccggcata
      901 ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt aacacatcgc
      961 tgccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacgat gacgaggatt
     1021 gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac cacacatgac
     1081 tgccattgga tgtgagtcgc gacggaaagc gtggaacaga cgggtaatct gctcgtggat
     1141 catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag tcggtttttc
     1201 accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca gctgatcgat
     1261 cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa ttttagattc
     1321 gcaggatgcg gttgaagtga agccagggtc aaaggtgaac acaccttttg aaccgagagt
     1381 acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt caacagctgt
     1441 gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc ttagcgcctt
     1501 attgcgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc tttacccgtt
     1561 tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag ctctgggcgc
     1621 ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt agcaatccga
     1681 attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat acttccacac
     1741 tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc attatatgct
     1801 tttcctggta atgtttgtaa caactttgtt gaatgattgt caaattagat gattaaaaat
     1861 taaataaatg ttgttatcgt gacctggatc actgttcagg ataaaacccg acaaactata
     1921 tgtaggttaa ttgtaatgat tttgtgaacg gcctatactg ccgccaggtc tccggaacac
     1981 cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg aagcagtgtt
     2041 ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac cggaggaagg
     2101 aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc cccagggaat
     2161 aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca aagacctgtt
     2221 aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat tctccatcgc
     2281 gtttccggtg tgatcacctt tgttgcagtg ggtatcctgc tgtggcttct gggtaccagc
     2341 ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt cttcgtcaaa
     2401 tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg tattcgccac
     2461 atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg ctccgccaaa
     2521 atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt atggtaagca
     2581 acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc gctaccgcta
     2641 tcgtcctgac gctctacatc atttatatgg tcggtttttt cgccaccagt ggcgagctga
     2701 catatgaagt ctggatcggt ttctgcgcct ctgcgttcac caaagtgttc accctgctgg
     2761 cgctgttttc tatcttgatc catgcctgga tcggcatgtg gcaggtgttg accgactacg
     2821 ttaaaccgct ggctttgcgc ctgatgctgc aactggtgat tgtcgttgca ctggtggttt
     2881 acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag tcagagaatt
     2941 tgatgcagtt gtgattggtg ccggcggcgc aggtatgcgc gcggcgctgc aaatttccca
     3001 gagcggccag acctgtgcgc tgctctctaa agtcttcccg acccgttccc ataccgtttc
     3061 tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact gggaatggca
     3121 tatgtacgac accgtgaaag ggtcggacta tatcggtgac caggacgcga ttgaatatat
     3181 gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc cgttctcgcg
     3241 tctcgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa acttcggcgg
     3301 cgagcaggcg gcacgcactg cggcagcggc tgaccgtacc ggtcacgcac tgttgcacac
     3361 gctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt atgcgctgga
     3421 tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc gcactgtgca tcgaaaccgg
     3481 tgaagtggtt tattttaaag cccgcgctac cgtgctggcg actggcggag cagggcgtat
     3541 ttatcagtct accactaacg cccacattaa caccggcgac ggcgtcggca tggctatccg
     3601 cgcaggcgta ccggtgcagg atatggaaat gtggcaattc cacccgaccg gcattgccgg
     3661 tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc tgaacaaaca
     3721 tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg gccgtgatgt
     3781 ggtggcgcgt tcaatcatga ttgaaatccg tgaaggtcgc ggctgtgatg gtccgtgggg
     3841 gccacacgcg aaactgaaac tcgatcacct gggtaaagaa gttctcgaat cccgtctgcc
     3901 gggtatcctg gagctttccc gtaccttcgc tcacgttgat ccggtgaaag agccgattcc
     3961 ggttatccca acctgtcact acatgatggg cggtattccg accaaagtta ccggtcaggc
     4021 gctgactgtg aatgagaaag gcgaagatgt ggttgttccg gggctgttcg cggtaggtga
     4081 aattgcctgc gtatcggtac acggtgctaa ccgtctgggc ggcaactcgc tgctggacct
     4141 ggtggtcttt ggtcgcgcag cgggtctgca tctgcaagag tctatcgccg agcagggcgc
     4201 attgcgcgat gccagcgagt ctgatgttga agcatctttg gatcgtctga accgctggaa
     4261 caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag aatgtatgca
     4321 gcataacttc tcggtcttcc gtgaaggtga tgcgatggct aaagggcttg agcagttgaa
     4381 agtgatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg agttcaacac
     4441 ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg caacggctgt
     4501 ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact tcccggatcg
     4561 tgatgatgaa aactggctgt gccactccct gtatctgcca gagtcggaat ccatgacgcg
     4621 ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga ttcgtactta
     4681 ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata acccggatgt
     4741 tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc gcgacatgat
     4801 gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt tccgccgctc
     4861 ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga atggtctggc
     4921 ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga ttcgcccgct
     4981 gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct atgcgcaata
     5041 tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc gcgagcattt
     5101 acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc tctgcgcatg
     5161 ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg gcccggcagg
     5221 cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg acagccgcct
     5281 cgacggtttg agcgatgcat tcagtgtatt ccgctgtcat agcatcatga actgcgtcag
     5341 tgtatgtccg aaggggctga acccgacgcg cgccatcggc catatcaagt cgatgttgtt
     5401 gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca agcgtcgcat caggcaacca
     5461 gtgccggatg cggcgtgaac gccttatccg gcctacaagt cattacccgt aggcctgata
     5521 agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc tttaaaaact gcccctgaca
     5581 ctaagacagt ttttaaaggt tccttcgcga gccactacgt agacaagagc tcgcaagtga
     5641 accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc ataaaaaaga
     5701 tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact cttcttacct
     5761 ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa ccgatcctga
     5821 ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg gagtcaaacc
     5881 ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag acgcttcacg
     5941 ttactcttca acgatctccg accctgacac caatgtgaag caggtaaaag tcctgcagct
     6001 cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc tgggactgtg
     6061 gcagcaagat aaagtggccg atctggatcc gtctttccac gatctgaccg aagcagactt
     6121 ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaccatga aactcggcga
     6181 gctgctggaa gccctcaagc aaacctactg cggcccgatt ggtgccgagt atatgcacat
     6241 taccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc gcgcgacttt
     6301 caatagcgaa gagaaaaaac gcttcctaag cgaactgacc gccgctgaag gccttgaacg
     6361 ttacctcggt gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg gtgacgcgtt
     6421 aatcccgatg cttaaagaga tgattcgcca cgctggcaac agcggcaccc gcgaagtggt
     6481 tctcgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg gtaaaaaacc
     6541 gcaagacttg ttcgacgagt ttgccggtaa acataaagaa cacctcggca cgggtgacgt
     6601 gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg tacatctggc
     6661 gctggcgttt aacccgtctc accttgagat tgtcagcccg gtcgttatcg gttctgttcg
     6721 tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa tcaccattca
     6781 cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca tgtcgaaagc
     6841 gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg ttggcttcac
     6901 cacctctaac ccgctggatg cccgttcaac gccgtactgt actgatatcg gtaagatggt
     6961 tcaggcaccg atttttcacg ttaacgcgga tgatccggaa gccgttgcct ttgtgacccg
     7021 tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc tggtgtgcta
     7081 ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccgc tgatgtatca
     7141 gaaaatcaaa aaacatccga cgccgcgcaa aatctatgct gacaagctgg agcaggaaaa
     7201 agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg cgctggatgc
     7261 tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca cctggtcgcc
     7321 gtacctcaac cacgaatggg acgaagagta cccgaataaa gttgagatga agcgcctgca
     7381 ggagctggcg aaacgcatca gcacggtgcc ggaagcggtt gaaatgcagt ctcgcgttgc
     7441 caaaatttat ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg actggggcgg
     7501 cgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc attccggttc gcctgtcggg
     7561 tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca accagtctaa
     7621 cggttccact tacacgccgc tgcaacacat ccataacggc cagggcgcgt tccgtgtctg
     7681 ggactccgta ctttctgaag aagcagtgct ggcgtttgaa tacggttatg ccaccgcaga
     7741 accacgcact ctgactatct gggaagctca gttcggtgac ttcgccaacg gtgcgcaggt
     7801 ggttatcgac cagttcatct cctctggcga acagaaatgg ggccggatgt gtggtctggt
     7861 gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg cgcgtctgga
     7921 acgttatctg caactttgtg ctgagcaaaa catgcaggtg tgcgtaccgt ctaccccggc
     7981 acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc cgctggtcgt
     8041 gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg aagaactggc
     8101 gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga agggcgtgaa
     8161 gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacagc gtcgtaagaa
     8221 caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc cgcataaagc
     8281 gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt gccaggaaga
     8341 gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag tgattccgtt
     8401 tggggcttct ctgcgttatg caggccgccc ggcctccgcc tctccggcgg tagggtatat
     8461 gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg tcgaataaat
     8521 aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga atccgtagcc
     8581 gatgccaccg tcgcaacctg gcataaaaaa cccggtgacg cagtcgtacg tgatgaagtg
     8641 ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc agacggcatt
     8701 ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat ccttggtcgc
     8761 ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca aatctgaaga gaaagcgtcc
     8821 actccggcgc aacgccagca ggcgtctctg gaagagcaaa acaacgatgc gttaagcccg
     8881 gcgatccgtc gcctgctggc tgaacataac ctcgacgcca gcgccattaa aggcaccggt
     8941 gtgggtggtc gtctgacccg tgaagatgtg gaaaaacatc tggcgaaagc tccggcgaaa
     9001 gagtctgcac cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc acgtagcgaa
     9061 aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct ggaagcgaaa
     9121 aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat tatggatctg
     9181 cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg ctttatgtcc
     9241 ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa tgcttctatc
     9301 gacggcgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt ttctacgccg
     9361 cgcggcctgg tgacgccggt actgcgtgat gtcgataccc tcggcatggc agacatcgag
     9421 aagaaaatca aagagctggc agttaaaggc cgtgacggca agctgacggt tgaagatctg
     9481 accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat gtctacgccg
     9541 atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga tcgtccgatg
     9601 gctgtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc ctacgatcac
     9661 cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga gttgctggaa
     9721 gatccgacgc gtctgctgct ggacgtgtag tagtttaagt ttcacctgca ctgtagaccg
     9781 acgtggatag ggtgtcggtc tacggtttaa aagataacga ttactgaagg atggacagaa
     9841 cacatgaact tacatgaata tcaggcaaaa caactgtttg cccgctatgg cttaccagca
     9901 ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc aaaaatcggt
     9961 gccggtccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa agcgggcggt
    10021 gtgaaagttg taaacagcaa agaagacatc cgtgcttttg cagaaaactg gctgggcaag
    10081 cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat tctggttgaa
    10141 gcagcgaccg atatcgctaa agagctgtat ctcggtgccg ttgtagaccg tagttcacgt
    10201 cgcgtggtct ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt ggcggaagaa
    10261 actccgcacc tgatccacaa agttgcgctt gatccgctga ctggcccgat gccgtatcag
    10321 ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca gttcaccaaa
    10381 atcttcatgg gcctggcgac cattttcctg gagcgcgacc tggcgctgat cgaaatcaac
    10441 ccgctggtca tcaccaaaca gggcgatctg atttgcctcg acggcaaact gggcgctgat
    10501 ggcaacgccc tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc gcaggaagat
    10561 ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga cggtaacatc
    10621 ggttgtatgg ttaacggcgc aggcctggcg atgggtacga tggacatcgt taaactgcac
    10681 ggcggcgaac cggctaactt cctggacgtt ggcggcggcg caaccaaaga acgtgtaacc
    10741 gaagcgttca aaatcatcct ctctgacgac aaagtgaaag ccgttctggt taacatcttc
    10801 ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggtgcggt agcggaagtg
    10861 ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact cggcgcgaag
    10921 aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga tgcagctcag
    10981 caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa aacaccaagg
    11041 ttatctgcca gggctttacc ggtagccagg ggactttcca ctcagaacag gccattgcat
    11101 acggcactaa aatggttggc ggcgtaaccc caggtaaagg cggtactacc cacctcggcc
    11161 tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgctacc gcttctgtta
    11221 tctacgtacc agcaccgttc tgcaaagact ccattctgga agccatcgac gcaggcatca
    11281 aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgacc gtgaaagtga
    11341 agctggatga agcaggcgtt cgtatgatcg gcccgaactg cccaggcgtt atcactccgg
    11401 gtgaatgcaa aatcggtatt cagcctggtc acattcacaa accgggtaaa gtgggtatcg
    11461 tttcccgttc cggtacactg acctatgaag cggttaaaca gaccacggat tacggtttcg
    11521 gtcagtcgac ctgtgtcggt atcggcggtg acccgatccc tggctctaac tttatcgaca
    11581 ttctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc ggtgagatcg
    11641 gcggtagcgc tgaagaagaa gcagctgcgt acatcaaaga gcacgttacc aagccagttg
    11701 tgggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggccac gcgggtgcca
    11761 tcattgccgg tgggaaaggg actgcggatg agaaattcgc tgctctggaa gccgcaggcg
    11821 tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt ctgaaataaa
    11881 tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaatggcga agggctgtcg
    11941 gtttcgacat ggttggccat cgtatgatgg ccttttttat gccttaaacg gttaaccacg
    12001 gtcgaaatct gactgcaggc ttgactgcat gaccacagcc actgtggatt gccgcaaccg
    12061 accgcaagcc acgaataaga acgacggcga atttcgaatc gttacctgat gcactggctt
    12121 cccagcgttt gaccgtagcg ttaagtaaat caacaaaagc gatgttcacc gcttttcgtc
    12181 aagtcgtgcc atttgtcagc caggatcggg tctttacccc ggatattgca gcatcgcaaa
    12241 aattcttaga gggatatgct ataaatcaaa aataaaatct ttgttgattg tttatcataa
    12301 tatccacaga tatgataaat gatatcaatt taataatttt cacttaatgt gattccggat
    12361 aattcgaaag gcgctgtgct tttcggatta ttattatctg attaagatat ttgtcacgcc
    12421 agccttactg gtttataaat gtcaatcatg attcttataa attatttatt gctgctttgg
    12481 ttaccattac ttcgtaataa gtaatgtatt tttgatggtt taaactattt ttaagggacg
    12541 gcattatgcg agggaaaata cctaaaacgg aactgctggt gacttttgag gtcgtcgcac
    12601 gtcatgaaag ttataccaga gcggcagagg agttagcgtt aacccaaagt gcagtattca
    12661 gacaagtaag tgcgctggag gaatttttac gtactccgtt attttcacac tctaaaaaac
    12721 gcatttttct taatgataca gggaagtatt acctggggat agtaaaagaa acattaaata
    12781 agctggaacg cgataccaat accattatga cctggcagcc tacggttcag gttattgaac
    12841 ttgccgttaa tcctactttt agtacccact ggttaatccc aaatttacat gaattcacaa
    12901 agttacatcc cgatattatt gttaatattc attctttagc taataacggc gattttctta
    12961 atcgggagta cgatgctgtc attatgcgcg aaaatttttg cgcgccctgg gcggaagtag
    13021 aatatctctt tgaagaggag attttgccgg tatgcagcgg cagtttgctt gcaatgtccg
    13081 atcaaaaact gtcagtggca gaactgctta ctgagttacc acttctgcat caaagcaccc
    13141 gtattactgg ctgggaagag tggtttgctt tatctggcgt tagcagtccg ctggtcaaca
    13201 acggaccacg tttcgatttg ctttcaatgc tgattgccgc cgtgcgctct aatctcggcg
    13261 tggcgctgtt accacgtttc gctattcaac atgatttgga tagtggcgat atggtgattc
    13321 cctgtgatgt gcctattcgt accggtaatc gttttattat gacgtggcag gaagagaagt
    13381 ctgactcacc gcacttgcag caatttcgtg aatggttgct ggcgaaatcg gtcgtaccgc
    13441 aggagatgta aaacctgcgc ctggcgggat gccaggcgca gttattacca gtcgataccc
    13501 ggctgggcct gaatgccgtt atcaaaagca tgtttgacgg ggcggatttc gctgaccgta
    13561 tctgccattt ttaatatctg actgtggcaa ccgcgtccgg tgacaatgac actttgttgt
    13621 gccgggcggt tttgcagaga agcgatcact tcttcggtat ccagataatg gtaagccagc
    13681 atataggtca gctcatcaag caccactaag tcgtaacgct tgtcagccag catccgtttg
    13741 ctttccgacc agacctcttt cgccgcgtcg atatccgctt gccggttctg tgtctcccag
    13801 gtaaaaccgg ttcccataat atgaaactca acccccagcg gctggagaag gttgtattcg
    13861 ccgttgtccc attgcccctt gatgtactgc gcaaccccca ccgttttgcc atgacccacc
    13921 gcgcgcgtca cagtaccaaa cgcggcagtg gatttccctt tgccgttgcc ggtgaaaaca
    13981 atcaagatcc cttttttctc tatcgccgcc gcgacgcgcg tatcgacctg ttccttcaat
    14041 ttctgttggc gctgcacatg ccgttcagtt gaaattcgcg cttccattca ttttccttaa
    14101 tgtcccgcag ggcaactggc gcagcggtgt tgctgctcaa actgactaaa gaaattattg
    14161 cctttatcat ctaccaggat gaatgcgggc agattttcca cttccatcat ccacaccgct
    14221 tccataccca gctccgggta ttccaggcaa cgcagacttt tgacatattc ttgcgccagt
    14281 aacgcagctg caccgccaat acttcccaga ttgaagccgc catgtttgtg acaggcatcg
    14341 gtgacctgct ggctgcggtt acctttcgat aacatcacta gactgccgcc agccgcctgg
    14401 aaggtatcga tataaccgtc cattcgaccg cccgtggttg gacccagtga accgcaggcc
    14461 atgttctccg gtgttttcgc cgggccagcg taatagacaa tatgatgctt caggtactcc
    14521 ggcatcggtt cgccgctatc cagccgcgct tttattttgg cgtgggcgat atcgcgcgcc
    14581 actacgatgg gaccacttaa cgacacgcgg gtgcctactg gtaatctggc gagatcctgc
    14641 atcacgtcac gcaacggacg gtttagatcc agttgtacat gttgcgcgtg attttcttcc
    14701 cgcagtgagg ctggaatata ttgccccgga ttgtgttcga gtttttccag ccagataccg
    14761 tgtttgttaa tttttgcttt gatattgcga tcggcagaac aggagagcgc catagctatc
    14821 gggcaagagc caccgtgacg cggcagacga atgacgcgaa tatcatgagc gaaatattta
    14881 cccccaaatt gcgcaccaat accaaattgc tgactggcct cgagcaacac tttttccagc
    14941 tcaatatcac ggaatgcttg tccctgctcg tttccgctgg tgggcaaatt gtcgtaatat
    15001 ttcgttgagg ccagcttggc aatttttaag gtttgatcgg ctgaaagccc tccgacgaca
    15061 aaggcgatgt ggtaaggcgg gcaggcagcg gtgcccagcg atttcatttt ttcgattaaa
    15121 aatgcggtca gtttttctgg ttgcaataag gatttggttt cctgataaag cgcggcttta
    15181 ttggcggagc cgcccccttt attaacacag agaaaatggt actcatcacc agcgacagcg
    15241 ctgatatcaa tctgcgccgg gagattggtt tgcgtattga cttcggtata catatccagc
    15301 ggcgcatttt gtgaaaagcg cagattattt tcctggaaag tactgtaaat gcctttactt
    15361 aaggcttcgg cgtcgttacc tccggtccat atctgttgcc ctttactggc aacaatagtt
    15421 gcggtgccgg tatcctgaca gttgggtaat accccttttg ctgagacttc agcattacgt
    15481 aataactgca acgcaacgta tttatcatta ctgctggcct gctgatcgtt aagaatgctg
    15541 gcgacctgtt gcaaatgagc ggaacgtaag aaaaaagagg cttcataaaa tgcctgccgg
    15601 gcaagcagcg ttaatgcttc aggagcgatt tttatcactt cttcgccgtc taattccgtt
    15661 actgtaatat gttgatcgct aattaatgta tattctgtac cgtctttatt ttgtagaaac
    15721 ggttcttgcc agataaatgg tttggacata tttcactttt caccttaaac gttagtgtta
    15781 cttaattgcc gtctaacttc atgatccggc ttataaaaat gcgtagtcat tggcttctga
    15841 ttgcttaaga tccccctcag ggcagagcaa cgttcgattc attgggtttt aatgctttac
    15901 cccgacagcc ctcgccgaaa tgttcgcctt cccaccgact caccatcgca gcgcccattg
    15961 cgttgctcat gacgtttgtc gcggaacgcc ccatatcaag gaacggatca acgcccatca
    16021 gcaagatcag ccccgcttcc ggaatattga actggttgag cgtggcggcg ataaccacca
    16081 ttgaggcgcg tggtacgcca gccattcctt tcgaggtcaa catcaggatc aacagcatgg
    16141 tgatttgctc accgatggat aaatggatat tgcaggcctg ggcgatgaaa actgtggcga
    16201 aggagcagta ggccattgat ccaacgagat taaatgagta gccaatgggt aagacaaagc
    16261 tggcaatttt gggggaaacg ccaaattgct ccagtttttc aagcgttccc ggaaaagccg
    16321 cttcagaact ggatgtggta aatgccagca gggcgggttc cgaaagggcg cgggtcaggc
    16381 gtctgatgca ggggccgaca taaacgatgg ccagaccgat aagcagtacc caaagtaata
    16441 acatggtgaa ataaaattca cccataaaga tcccggcgct caccataact gccagtcctc
    16501 gttcagcaat caatgctgaa atagcagcga atacggtcag gggagcgaag agcatgacgt
    16561 agccagtgag ctttaacatg gcatgtgcca gcgaatctaa ggcgtgaacg atggcgctgc
    16621 ctttctcgcc aatcgccgtc aggctacagc cgaggaaaat tgagaacacc acgatttgca
    16681 aaatttcatt gtgcgccatg gcatcgacaa tgctggtggg gaaagcatgc gaaataaata
    16741 cttttagcgt aaagggttct gccgcgacca ctccggtggt ttcggcaccg tgtgcaacaa
    16801 aattaattcc tgtacctggc atgaagaaat ttaccgttat caagcctaag gcaattgaca
    16861 gcaatgaggc gcaaataaat aaaaagagtg ttttagaaaa aatacgacca agggctttgg
    16921 catcgcccat tttagcaata cctaccacca gggtagagac cactaacgga gcgataatca
    16981 tttttatcag tcgtaagaaa atcgtcgtga atatcgatat ttcttgcgca tagagttttg
    17041 cggtttccgg tgaagcagtg ttattgagca ctacgccgat aatcattcca agtaccagcg
    17101 ccaaaagaat catcgtggtt aaacttattt tcttcatttt ttcctccatc aggtgcacgg
    17161 cgataaatca gaatcaggag aagtagatgc ctgaatagag attcaggcat ctacctgcta
    17221 ttacaacctt acaaatagct gaagtgttga actgcaataa gtgacgggat gtcagttggc
    17281 taaaagtatt cggattttta caaatcgaaa ctcatattta ataaaggtgc atgttgctgt
    17341 gcaccataga catcattgtc acctacattc ccggagataa tatctcgtgg catgacgatt
    17401 tttactgcgt tagaaggatc aaaccagaca atacgatgaa taaagtcagg ttcaacatga
    17461 tataagcgtg caatcagttc tggggttaat tgccctgatg atttaactcg ctcatagttt
    17521 tctttcgttt taaataaaat atctaaaacc agttcgtaag gtccggcatt tttcgaacga
    17581 atgacttgcg ccagtgagac aattgactgt ttcatgcctg aactccttct ggcgtcacct
    17641 gttcaatatg gaaatcaaaa cgcagagcgt cgttggcttc aatcaggtga tatatggaaa
    17701 attcgtatac cggcccgccc tggatatcag aaggggagaa cgggaacgcc agattacctg
    17761 cggtagcgat gcggttttca tagccgtagt gcagcatggt agagcgcacc agcgagcaaa
    17821 cgctgttggc aatttcctgg gtcggtgcaa ctacatcaag gacaatcccc agctcatgcc
    17881 cggcagtttg catcggttca tggtctccca tcaccccgtt tttgccgtac aggtggaaat
    17941 tgatgcgaat gctgtcatcg tcgagcgaca ggttacgcga aacgctggtt ttgacttcat
    18001 cgatgatttt atcgatcccg gcgatcataa tcgggtcgcg cgttccggcg atggtcagac
    18061 agcggaagcc cacccgtcgt gcaccttcca gtttcagggc atacggcgtt tcttcatgct
    18121 tagaaccgct gacgtagact tcgccgtcat ttactgcttt aaatgtgcag cctttcaggt
    18181 tcaacacgcc gccaggtcct ggcaggaagt agggatcgga tttctcatac agtgtgtgtg
    18241 cagctgctga cgtttcggta aatttacgct tcggattaaa tgttttcagc gtaaagccgt
    18301 tgtcatcaat gatgcccatc gcacagtcag agcctgagcc gggcgttgcg gcgattgccg
    18361 cgcattcaag gattttccca caatgcagcg ccagaccttc atcaaagcct tgcatgatcg
    18421 ggagcgcagc aaagcaggcc gggtcgtaag cgcgcccgcc cagcaccact tgcgcgcgtt
    18481 ctttcagcgc ccgctggaag ggttcgatgc ccatttgcgc gacgatatac gtactttcgt
    18541 caatcgcgtc gtgggttaac ggcgggacaa aatccagcgc cgtgattttg ccgttatcca
    18601 gcgcctggtg gacgatggct ttgtcaacgt ctgccgggat caacgccatc gagaacgaga
    18661 ggtgctcttc ccgggcaatc tcatgaatta tttctcggca ccactccagg tgtggtgcgg
    18721 caccagaacc gccagccgtt ccgatcacca ccggtatatt attttgcacg cctgcggtga
    18781 tcatatagcg cagatcgcgc ttgacccctg cgcgatcggt aaacggtttg cctgccccga
    18841 ggtagtgggg gcctggatcg gaagagcctg catcaacggc gatcagatcg ggcgattctg
    18901 ccatcgcttt gcggaagctc tcttccggga atccataccc gaggattgcc gttggcgata
    18961 agactttaaa agaacgagcc atttgttaat ccttagcctg caacagcgcg atagtacgat
    19021 tcatttcgtt gaagacgata tcgaggcctt cgtcaaagcc catccctggt tttaccagca
    19081 tacgcatggg gcgagcggca agggcgacgt ggacacaggt gcgggcactg acatcagttt
    19141 cattgcaggt gccgccctgg tacgcttcca tgctgtggct gttgcagtaa agaaccgcat
    19201 cgacgatgtt gtgaatactg cccagatccg gggttttgat ttgcaccatg tgacaactgg
    19261 cagcatcagt gaaatcaaca atatcctggt aggtgttaca ccattcatcg gccacaattt
    19321 tcacgccgga accgaggcgc gtcagctctt tggtaatcgc ggtcagcagg cgaatttgat
    19381 cgggcttgtt accggcatcg accggccctt cgatgtagag cggcaggcct tgcgcttctt
    19441 tttccaggct ggcgatgtat tgcgcacagc gaagcggatc catatcgaag atcagaccga
    19501 tagtgccgta tacatcgatg tgcagggtag ggtggtagcg tgcgctggtg cgcttgctta
    19561 gaatgcgatc cgacaaccag cggacatatt cgcgcagttt ttcaccttta aagcccagct
    19621 tctcttcgac gttattaatc agcgcatggg gcagcacgtc gatgccttta aggatcatct
    19681 tatcgacggc gatatatcga tcgtcgccgc tctgaccaaa taatggaatg gattccgcca
    19741 cgcgtggcaa ctgccattca tcacagacca cttcagtttt cagacggccg gttgccagcg
    19801 cggtagcatc aagcagcgcc tgtgataatc cgtagcgcac ggcggtatgc agcaagttgc
    19861 cgtcaatacg caatttgtcg aagaaacggg cattcggcag gaaagcatcc acatcgcggc
    19921 ctaccagtaa tggcttgata tggtcgttga ggaacggaat aaagtgctct gcgaggaaca
    19981 gtggatcgcg accgcctgcc ccggaatact gtacggcagt acagtcacct acggcgaccg
    20041 cgccgttttc cagaatcaac tgtaccgata cgcactcccc ggcctgacgt acggcgttaa
    20101 acccctgtgt tactggctcc ccggtataaa aaaagccgtc atgacccgct ccgtttttta
    20161 tcgcctgctg gtcatcgaaa tagaatgagg agtagccagc ggtgaacaga gcctgtttta
    20221 ttttcattat ggtcttccta ttaatttact gtgggataca gcattgatgt catctacaac
    20281 catctggaat gtcggttggc gtccttcaca gcgtgcgcgt tccgcaacgt aatcgtggtg
    20341 gaggtcgaga atatcttttg gcaatggaac ggctccggct tccagtacgc gtatcgcgcc
    20401 ggtgttgtca cggaccggaa gaattttgcc cgcgttgcag gcggctggcg caaacggcac
    20461 atccagtacg cccgcttcaa atgccagcac cgttccgcgc gcaatatcac cattacccag
    20521 ttcgaacact ttattcagta ccgcgcgaac ctcgctctta atcagttcaa tttccagttc
    20581 aaccgcaggg cacggcggga atttctgctc attgaccatg ttgagcattt ggcgggaggc
    20641 tttcagcccc tgaatgttgg cagcggcagt cgggataccc caggcttcgt gcggactttt
    20701 ggtgatgact ttggtggcac cagacatccc cgcgacggct gcgccccagg agataatagc
    20761 gaacgctttc gattcgtctt ccggaaagcc gcccatccac tggtgaaaca cggtactcaa
    20821 ttcatagtcg gtatagccat agctctgaaa atattcgtgt gcaagttcac gcagagactg
    20881 gatcgccgca acatcctgcg tcaggctgcc gacctggcca taaccaacgg tgatagattt
    20941 cacaccctgt tccagcgcca gcaagccttc gattatcgcg atagagtgcg agataaacgg
    21001 cgggatcagc gtacccgtta gtgggccaaa cggttcgcgg ttgatgcgga tcccgtgttc
    21061 ttcatacatc cccattaaac ggtcgcagta ctgccagtcg cggatcgatt tttccagcgt
    21121 tacgcgtttg gcatacggaa tgttgtagga aatgccaccg ccttcataac tggtaaagcc
    21181 gctggccatt gagatttcag ccagcagacg cgcatctggc gtgccgtggc ggatctgtag
    21241 cggtttttgc agggtttcgg tgaggcgacg gcaagctgcg acaccatggt taactaccgg
    21301 taaaccgttc agtttagagg ttccggcttc gatggatttt ttaatcccta cagccgcttc
    21361 ttcgtaccga ttcagacggg tatatgcatc gatggtgctt ggcagcagat cgcactcttc
    21421 ttgtagtgtt ttcagcagtt cgatgtgttc gtccatcagc gccacacctg cgcgcggttg
    21481 gctaagggtt ttaccttcct tgtctgcttt cagcagcgca agagagaaac gtttatgctc
    21541 agggatggtt tgttggtatt taacgccatc ttcaaagttt tcaacatcct tgccggtttc
    21601 ccaggttttc agtacctgtt gccgctcggt catgaattcg tcatgggtta atttcttatt
    21661 tcgaagttcc attgattgca gccttaattg ttatagggtt aagcattgaa tactggtgcg
    21721 cgcggcggcc agcggatcga ctctggcgac gtttgccagc agtggtaata gcccccggga
    21781 atcgcggtaa tattcaaatt cggtcggtaa gagaatgcgt ttaccgtcgt cgtttaactc
    21841 gcggtatttc aaccagtgat gcatatcgaa ctgactggca cgcgagagcc atccgccgga
    21901 tccgacgact ttacgcacgg ttgtcaggtc acgtcccatc tgtaaatcga cattgccgac
    21961 gcaggtgcag acctcttttt tggtgcccgc atgtctttcg gtggcataac ccacacacag
    22021 tccggccagg acggtatcga aatatttctc ttctgcattt gcgggtaagt agtcgggatg
    22081 agcgactaga tgacggaggt aatgataaaa agcgtccagc tgtgcgggtt gctgagcaaa
    22141 attcacattg actaactctt tcgcactttc accaaccacc actgccgaga cgcgcattcc
    22201 cagatcgcct tccaccgtcc gttttacgaa gggttccggt acgccgtgta aaacggtgtc
    22261 gggcgagagc gtgttggcac aggcggaata aacatcggtg gttgcgccgc ccatgtcgat
    22321 cagcatgaac tctttccagc tgtgatcgac gttactgatt gccttgacca gttcaaagac
    22381 cgtccatggt gtcggcattg gttcttcgcc tgttttatca acaatgacat ccagcccttt
    22441 ccctttaacg atgcgtttaa ggaatatgtc gcaaatagcc tggcgtgcag ctacagggtt
    22501 cggatggtca agatcgggca gaacgttgtc gacaatggtc aggtctttat gcccgaggat
    22561 ctcctgcacc tcgtcctgaa tatcccggtt tccggcataa ataatggcgc agtcgagttt
    22621 ggattctgcc agcacacggg cgttattcag tccgtagctt tcttctccgc cgtcggtgcc
    22681 gccggtgaac aagagaatat cgggctgcgt ttcttccagt gcctggatat cacggcggtt
    22741 cagcttgtag gcgtaatatt gcgcaatttt agcacccgca gagtgagcgg ttactttcgc
    22801 ggtttccagg gtgatagaag gcaccaatcc cattgcggca accgccagcc cacctttggc
    22861 agaggaggaa tacttcaatg caacctcacc gctgtttaac agggggaggg cattatctac
    22921 gtttaacacc tggttcaggc tggagaaaaa ccccttcgcc agatgatggg gggtggttgg
    22981 ggtcagtacg tggttaacca acgttaacgc gtccccctcc cgggtaaaga gggctgcttt
    23041 ggtccacgtc gatccaatat cgacagagac gatttgcatc agatagcctc ttcaagaata
    23101 cgagtatcaa cgtcatgacg ttggttgata tcgtgggcca tcagctggca aacatcttct
    23161 aagtcatgac ttggggcaaa gacgcgatca aatcccatct ctttgaattt ggtttccacg
    23221 tcagcgaagt catgttttcc gaccaccagg ttaccgccca catagagcag gatgtccccc
    23281 agtccacgct cgatgcagcg ttcgcgcatg cccagacagt cgatatcgcc atgcccatag
    23341 atagaggaga cgacaatcgc gtcagcgcca gtttcaatgg cagcatcaat atattcgtcc
    23401 tggctgacca ttacgcccag attaatgact ctgaagtcgt ggttgctaaa aacgcggtcc
    23461 agaactttat tgcctactgc atggcagtcc gcgccaataa cgccaatcac aagtgttgct
    23521 ttcttcatgg gtaatccttt gtaagacaga gagtaaaata agttacacac gtcgtattta
    23581 tggccgttca taacggcaca ggtttaatat cacaggagtg tctttatctg accgtaatgt
    23641 tgcaattgat ctattttcaa aatgtctttc gggaaatgat tgtttgaagt gtgtttttaa
    23701 atttccatgt taa
//
LOCUS       DAZFDC010000070        23517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFDC010000070 DAZFDC010000000
VERSION     DAZFDC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.070"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            73..300
                     /locus_tag="ACHJ48_002840"
     CDS             73..300
                     /locus_tag="ACHJ48_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708359.1"
                     /translation="MACNVFAFSNSGGGSETSYKEPEKTSAMTTTHSTKFQPSQAILF
                     KMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(377..619)
                     /gene="yfdY"
                     /locus_tag="ACHJ48_002841"
     CDS             complement(377..619)
                     /gene="yfdY"
                     /locus_tag="ACHJ48_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO4708360.1"
                     /translation="MFYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            972..1892
                     /gene="lpxP"
                     /locus_tag="ACHJ48_002842"
     CDS             972..1892
                     /gene="lpxP"
                     /locus_tag="ACHJ48_002842"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO4708361.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPIMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2248..2319
                     /gene="ypdK"
                     /locus_tag="ACHJ48_002843"
     CDS             2248..2319
                     /gene="ypdK"
                     /locus_tag="ACHJ48_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO4708362.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2384..3622)
                     /gene="alaC"
                     /locus_tag="ACHJ48_002844"
     CDS             complement(2384..3622)
                     /gene="alaC"
                     /locus_tag="ACHJ48_002844"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO4708363.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAILESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            3999..5696
                     /gene="ypdA"
                     /locus_tag="ACHJ48_002845"
     CDS             3999..5696
                     /gene="ypdA"
                     /locus_tag="ACHJ48_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO4708364.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5711..6445
                     /gene="ypdB"
                     /locus_tag="ACHJ48_002846"
     CDS             5711..6445
                     /gene="ypdB"
                     /locus_tag="ACHJ48_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO4708365.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6458..7315
                     /gene="ypdC"
                     /locus_tag="ACHJ48_002847"
     CDS             6458..7315
                     /gene="ypdC"
                     /locus_tag="ACHJ48_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4708366.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7318..9813)
                     /gene="ptsP"
                     /locus_tag="ACHJ48_002848"
     CDS             complement(7318..9813)
                     /gene="ptsP"
                     /locus_tag="ACHJ48_002848"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO4708367.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIIRNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSEPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHGLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9838..10875)
                     /gene="ypdE"
                     /locus_tag="ACHJ48_002849"
     CDS             complement(9838..10875)
                     /gene="ypdE"
                     /locus_tag="ACHJ48_002849"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4708368.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFNYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10875..11960)
                     /gene="ypdF"
                     /locus_tag="ACHJ48_002850"
     CDS             complement(10875..11960)
                     /gene="ypdF"
                     /locus_tag="ACHJ48_002850"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO4708369.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEVRTQGYQLHLLDATHTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDEAARRV
                     ITEAGFSHYFGHNTAHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11975..13222)
                     /gene="fryC"
                     /locus_tag="ACHJ48_002851"
     CDS             complement(11975..13222)
                     /gene="fryC"
                     /locus_tag="ACHJ48_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO4708370.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13244..13570)
                     /gene="fryB"
                     /locus_tag="ACHJ48_002852"
     CDS             complement(13244..13570)
                     /gene="fryB"
                     /locus_tag="ACHJ48_002852"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO4708371.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13789..14754)
                     /gene="glk"
                     /locus_tag="ACHJ48_002853"
     CDS             complement(13789..14754)
                     /gene="glk"
                     /locus_tag="ACHJ48_002853"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO4708372.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14958..16214
                     /gene="yfeO"
                     /locus_tag="ACHJ48_002854"
     CDS             14958..16214
                     /gene="yfeO"
                     /locus_tag="ACHJ48_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO4708373.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16329..16655
                     /gene="ypeC"
                     /locus_tag="ACHJ48_002855"
     CDS             16329..16655
                     /gene="ypeC"
                     /locus_tag="ACHJ48_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO4708374.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16795..18033)
                     /gene="mntH"
                     /locus_tag="ACHJ48_002856"
     CDS             complement(16795..18033)
                     /gene="mntH"
                     /locus_tag="ACHJ48_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO4708375.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18369..19571
                     /gene="nupC"
                     /locus_tag="ACHJ48_002857"
     CDS             18369..19571
                     /gene="nupC"
                     /locus_tag="ACHJ48_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO4708376.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ACEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WIGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19621..21810)
                     /gene="pdeA"
                     /locus_tag="ACHJ48_002858"
     CDS             complement(19621..21810)
                     /gene="pdeA"
                     /locus_tag="ACHJ48_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO4708377.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEVLARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(22018..22093)
                     /locus_tag="ACHJ48_002859"
     tRNA            complement(22018..22093)
                     /locus_tag="ACHJ48_002859"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22058..22060),aa:Ala,seq:ggc)
     gene            complement(22133..22208)
                     /locus_tag="ACHJ48_002860"
     tRNA            complement(22133..22208)
                     /locus_tag="ACHJ48_002860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22173..22175),aa:Ala,seq:ggc)
     gene            22429..22788
                     /gene="yfeC"
                     /locus_tag="ACHJ48_002861"
     CDS             22429..22788
                     /gene="yfeC"
                     /locus_tag="ACHJ48_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4708378.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22790..23182
                     /gene="yfeD"
                     /locus_tag="ACHJ48_002862"
     CDS             22790..23182
                     /gene="yfeD"
                     /locus_tag="ACHJ48_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO4708379.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23222..>23517)
                     /locus_tag="ACHJ48_002863"
     CDS             complement(23222..>23517)
                     /locus_tag="ACHJ48_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4708380.1"
                     /translation="EMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQS
                     KFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         5838 a         6234 c         5806 g         5639 t
ORIGIN      
        1 gaaccatttt caggataact ttctatgaaa gtaaacttaa tactttttag cttattttta
       61 ttggtctcta ttatggcatg caatgttttt gcattttcta attctggtgg tggtagtgag
      121 acgagctata aagagccaga aaaaacgtca gcgatgacga caactcactc tacaaaattt
      181 cagccatcac aggcgatttt gtttaagatg agagaagatg caccaccatt aaacctcaca
      241 gaagaaacca cgcccccttt tccgacaaag gcgaattatc ttattcatcc tgtgcgatag
      301 cgtctgtcac ctggcaggtg aacaatcatg tctgatgctg atgtgaacac ctgcccaaat
      361 gaaccatcct gacccactat ctcctgccat accccaccat aaagcgaatc atcaagccac
      421 aagccggggc aagaaacata aatatccccg tcaccagttc attaccgcca gttaaccaca
      481 tggtgaagta ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg
      541 ataccacgtt cagtacctga ccgatgtatc cgcttacgca gacaatacac agggcgagaa
      601 acatccataa ataaaacata gctcaacctc ctgccgcgct aaaaaacctg taacttcagt
      661 cactttaata gtaagacaat ccttaacctc tggcaatttt tgctaagcgt caatgaaaac
      721 agcatgttaa atgcaatact attgcgtgcg gaaaattttt tactttgcac gattaataat
      781 cagtagctga aagcagtcag ccagtgataa agtacagata ccgcattcat gctcctcgtg
      841 gttatgtcct gactagtctt tacactcttt acaggaacca ttgtcgtaca tgatggccca
      901 accaattgag tgtaccgctg tcgacatgga atcttctgcg atacaacaat tcgtatctac
      961 agaaggtaac tatgtttcca caatgcaaat tttcccgcga gtttctacat cctcgctact
     1021 ggctcacatg gtttgggctt ggtgtactct ggctttgggt acagcttcct tatcctgttc
     1081 tctgctttct cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa cggcgtgaat
     1141 ctatcgcccg taaaaacctg gaactttgct tcccgcagca ttctgcggaa gaacgcgaga
     1201 agatgattgc cgaaaacttt cgttcactcg gcatggctct ggtagaaacc ggcatggcgt
     1261 ggttctggcc cgacagtcgc gtacgtaaat ggtttgatgt tgaagggctg gataacctta
     1321 aacgcgcaca aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg
     1381 aactgggcgg ccgcgtgatg ggactttgtc agccaataat ggctacctat cgtccacata
     1441 ataatcagct gatggaatgg gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga
     1501 tcggcagaaa taatctgcgc ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt
     1561 ttgctcccga tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg
     1621 aaaatgtcgc cacaaccaat ggcacctatg ttctctcccg tctctctggc gcagccatgt
     1681 tgaccgtaac gatggtaaga aaagcggatt acagcggata tcgtttgttc atcaccccag
     1741 agatggaagg ctacccgaca gatgaaaatc aagccgctgc ctatatgaac aagattatcg
     1801 agaaagagat catgcgcgca ccggagcagt acctctggat ccaccgtcgc tttaaaacgc
     1861 gcccggtggg agaatcgtcg ttgtacattt aacaaactgc tcttactctt tccctcctca
     1921 aatcggccat aataacctca tggtttttat ggcttttttc ttaagatttt ttccttcgct
     1981 gaatatctaa ttattaccgc cagaaattaa actgtcgccg atatgagaca cctgtaagtt
     2041 actaaaagta aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc
     2101 cgtctcgagc agtttagctc agaattctgg ggtgatgccc acggtaaaat acaggacttt
     2161 taaggtcgtt ttgcccggtg tcaactcact gtatcaggtg tgatgaagtc attcaggcgt
     2221 aaccgtaatt acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg
     2281 gtcatcgttt gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa
     2341 acaggagcca tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt
     2401 gaatatgttt gctgctggcg ggtagtaaac cgtcggcacg gaacatcgct ttaatcccac
     2461 gaatcgcctg acgaatacgg tcgcggtttt caatcagtgc aaagcgaaca tgcgtgtcgc
     2521 cgtagtcacc aaagccaatc cctggagaga cacagacctt cgcttcgtta aggagcttct
     2581 tggcaaattc cagcgatccc atggccgcat atggttccgg gattttcgcc cagacataca
     2641 tcgaagcctt cggcatttcg accatccagc ccgcttcatg cagcccttta accaatacat
     2701 cgcggcggcg tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg
     2761 ccgcaatcgc cgccacctgc aacggcgtaa aagtgccgta atcgtgatag cttttgattc
     2821 gcgccagggc actgaccagc gttttgttgc cgaccataaa gccgatacgc cagcccgcca
     2881 tgttgtagct tttcgacagc gtaaagaact cgactgccac atcgcgtgca cccggtacct
     2941 gcatgattga cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcatgga
     3001 ccaccagcac atcgtagcgt ttcgccagcg ccactacttt ttcgaagaac tccagttcca
     3061 cgcattgcgc ggttgggttt gacgggaagc cgaggatcat catcttcggt ttcggataac
     3121 tttcaagaat ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg
     3181 agcgtacctg cgccccggca atcaccgcgc cataaatatg gatcgggtag cttggattcg
     3241 gcaccagcac cgtgtcacca tgatccagcg tcgccagcat caggtgtgcc agcccttctt
     3301 tcgaaccaat ggtgacgatg gcttctgatt ccggatcgat ttcaacgtcg tagcgatcct
     3361 gataccagcg ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat
     3421 gcgtgtccgg acgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac
     3481 cgtccgggtt acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt
     3541 tcagttcagc ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac
     3601 gttcagggcg agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga
     3661 gcgacgctgc cacgatggtg gctgttttga aaatagcctg attaatttct gtctgtccag
     3721 cgttttagaa catttttatt aatgacgtta aatgaggaac tctgctttaa aaacaggaaa
     3781 tttatctgcg ctaacaagaa aattaccact cattttatta acataataat ttcaataaat
     3841 ttactaacca gaagtgcatt atcaagagat gcattgctaa tacaccattt atctatcaat
     3901 ccccttttaa aattgtggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt
     3961 acctatcata gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc
     4021 tgctggcggt cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta
     4081 tccgcctgtt tcgcgaactg ttgcacaagt cggcacactc accgaaagag ttgctcgccg
     4141 ttaccgccat tttctcgctg ttcgccctct tcagcacctg gtccggcgtt cccgtagaag
     4201 gctcgctggt gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt
     4261 gggtgggcat cattaccggc gtgattgcgg gtattcatcg ttatttaatt gatatcggtg
     4321 gcgtgacggc gatcccctgc tttatcacca gcattctggc tggttgtata tcgggctgga
     4381 tcaacctgaa aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt
     4441 gtgaaacgtt gaccatgatt ctggtgattg tctgggcacc aactaccgcg ttggggatcg
     4501 atatcgtctc taaaatcggg attccaatga tcctcggcag cgtctgtatc ggctttattg
     4561 tgcttctagt gcaaagcgtt gagggcgaaa aagaggccag cgccgcgcgg caggccaagt
     4621 tggcgctgga tatcgccaac aaaacgctac ctctgtttcg ccatgtcaat agcgagtcat
     4681 tacgcaaagt ttgtgaaatt attcgcgatg acattcacgc cgatgcggtg gcgattacca
     4741 ataccgatca tgtgctggcc tatgttggtg tgggtgaaca taactatcag aatggcgatg
     4801 acttcattag cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca
     4861 atgatgaagc ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga
     4921 aaggcgttgt gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt
     4981 cattacagga aatggcggtt ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac
     5041 gcgccgagca gctacgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa
     5101 ttaatcccca tttcctgttt aacgctttga acgctatttc atcgtcgatc cgtctgaatc
     5161 cggataccgc tcgccagctg atctttaatc tgtcgcgtta tctgcgctat aacattgaat
     5221 taaaagacga tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg
     5281 ccattgagca ggcccgcttt ggcgacaagc tgacggttat ctatgatatt gatgaagagg
     5341 tgaattgctg cattcccagc ctgctgatcc agccgttggt ggagaatgcc attgtccacg
     5401 gtatccagcc ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggcaatc
     5461 gggtacgcat tgcggtgcgg gataccgggc acggcatcga tccaaaggtg attgagcggg
     5521 tcgaagcgaa tgaaatgccg ggcaataaaa ttggcctgct gaatgtccat catcgcgtga
     5581 agttattgta tggcgagggg ctgcatatcc gccgcctgga accaggaacg gaaattgcgt
     5641 tttacattcc taaccaacgc accccagtcg cctcacaggc tacgttattg ctttgagccg
     5701 gagtgatatt gtgaaagtca tcattgttga agacgaattc ctggcacaac aggaactgag
     5761 ctggctaatt aaagagcaca gccagatgga gattgtcggc acctttgacg acggtctgga
     5821 cgtgttgaag tttttgcagc ataaccgcgt cgacgccatt tttctggata tcaatattcc
     5881 gtcgctggat ggtgtgttgc tggcacaaaa catcagccag ttcgcccata aaccgtttat
     5941 tgtgttcatc accgcgtgga aagaacatgc ggtagaagcg tttgaactgg aggcgtttga
     6001 ctacattcta aaaccgtatc aggagtcacg cattaccggg atgctgcaaa aactggaagc
     6061 agcctggcaa caacagcaga ccagcagcac gcctgccgcg acggtaacgc gtgagaatga
     6121 caccattaat ctggtgaaag atgagcgaat aatcgtcacg ccaattaacg atatctatta
     6181 cgccgaagcg cacgagaaaa tgacctttgt ttatacgcgg cgtgaatcct acgtaatgcc
     6241 gatgaacatt accgaatttt gcagcaaact gccgccgtcg cattttttcc gctgccatcg
     6301 ctcattttgt gtcaatctga acaaaatccg cgaaatcgaa ccgtggttta ataacaccta
     6361 tattctgcgg ctgaaagatc tggattttga agtgccggtc agccgcagca aagttaaaga
     6421 attccgccag ttaatgcatc tttaatcggg aactttcatg aaagcaccag gcttgcccgc
     6481 cgatcaacaa tttttcgccg atctgttcag cggcctggtg cttaacccgc aactactggg
     6541 gcgcgtctgg tttgccagcc agcctgcctc gttgccggtg ggcagtttat gtattgattt
     6601 tccccgtctg gatatcgtgc tgcgcggcga atacggcaat ctgctggaag caaagcagca
     6661 acgtttggtg gaaggagaaa tgctgtttat tccggcgcgc gcggctaatt taccggtcaa
     6721 caacaaaccg gtgatgctgt taagcctggt gttcgctccg acctggcttg ggttatcgtt
     6781 ttacgatagc cgcaccacgt cattgttgca tcctgctcgc cagatccagc ttcccagcct
     6841 gcaacgcggt gaaggtgaag cgatgcttac cgccctcacc catcttagcc gttcgccgct
     6901 ggagcaaaat atcattcagc cgctggtgtt aagtttgctg catctttgcc gtaacgtggt
     6961 gaatatgccg ccgggcaatt cgcagccgcg cggcgatttt ctctatcaca gcatttgtaa
     7021 ctgggttcag gataattatg cccagccgct cacccgcgag agcgtggcgc agttttttaa
     7081 tatcacgccc aatcatctgt caaaactgtt tgctcagcat ggaacgatgc gttttatcga
     7141 gtatgtgcgt tgggtgcgaa tggcgaaggc gaggatgatt ttgcagaaat atcatttgtc
     7201 aattcatgaa gtggcacagc gttgcggttt tccggatagc gactattttt gtcgcgtttt
     7261 ccggcgtcag tttggtctga cgccgggaga gtacagcgcc cgttttcagg gctaacgtca
     7321 gaaggttaat tctgtttcca gcagcgtcag gatactttgt gcatcttgcg cggcaaacaa
     7381 cgactggcgg aagtttttat tcaccagttt acgcgccagc tgcgagaaga ctttcacatg
     7441 attcatccct tcgttagcac ccagcgtcag catgatcacc agttcgactt cgcccatttc
     7501 tgactgccag tcaaccggtt tcgccagccg ggcaatgctg atactggaat gacggatcca
     7561 ctgagatttg gtgtgcggga tcgccacgcc aaaaccaacg ccagtggtga caatctcttc
     7621 ccgctgccag acatcctctt ccagctcgaa cggatgttca gtgcgcccgt taacgccgag
     7681 gttaccgcac aggaactgga tcgcctgctc tttattgcta aaggcctgat caacgaagat
     7741 attctccagc gccagcagcg ggcgaacgtc ttcttccggc gtaaaggcgg tgagtaacgc
     7801 ttcaatttcc tgcgcgctgc gacattcaca cgcctgacgt gccagttccc gacacgcctc
     7861 gctatccagt tgacgaagct ggcttttcac cgccggaatg cgcgggctac tcatactcag
     7921 ctcatccagg cccagcccaa gcagtagcgg cagataacgg ctttcaccgc ccagttcacc
     7981 gcaaatgcct acccatttgc cccgctgatg cgcagtggta actatttgct gcaacatgcg
     8041 caggaacgat ggcgtaatcg ggttatatag cggcgatacg cgcgggttat tacgatcgac
     8101 cgcatacaga tactgggtca tatcgttgga gccgatactg aagaaatcga cctcatcgca
     8161 gaagtgatcg atgatgtagc acaccgacgg cacttccacc atgataccca gcgtaatcgt
     8221 ctctgcatga cgcaggccat cgcgcttaag ctcaacgatc gctttttgga tctcgccctt
     8281 cacccataag atctgatcga ggccgtggac catcgggatc atcaactggg cgttgccgaa
     8341 actggcggcg cgcaaaatgg cccgcagttg agtgcggaac aggccagcaa attccgggta
     8401 aatacgtacc gcgcgatagc cgaggaacgg gttctcttcc tggggaatat tcagataagg
     8461 aatgctttta tcgcctccga tgtccattgt gcggaagata atcggcttgt cgcccgctgc
     8521 cagtagcacc tgctggtagg cttcaaattg ctcctgctcg tccggctcgc tgtcgcgatc
     8581 catatagagc atttcggtac ggaacagccc gacgccttcc gcgccgttgg caaacgcgcc
     8641 aggcgcttcc agagcggtgc caatattcgc cgcgatatca atacgcttgt tatcacggga
     8701 ataagccagc tgcgcggcag cctgcgcctg ttgtttttgg cgtttatccg ccagcgtctg
     8761 cgcgacctga taataaccgc tcaccgcgtc attcgggtta atcgccagca cgccgcactg
     8821 ggcgtcaagc actgcaggtt gcccggcata acgcgcgatc gcatccagcg gcaggccgct
     8881 cagtaccggg atcgccgagg cgcgggccag aatcagggta tgcgaggtgc gcccggtttt
     8941 ctccagaatc atgcccgcaa gattttttaa atccaggctt aaaaactggc ttggggttaa
     9001 atcctcggcc accagaatag tcggtttttc cagcaccaga ttgttgcgcg gcttcagttc
     9061 cggccaggta atatgcagca actgttcgct aatatcgcga atgtcgctaa cacgttcgcg
     9121 cagataatcg ctggcagaag cagaaagttt ggcgcaaacc tgctccatat ttctgatgat
     9181 cgccgccccc agcccctgat gctgttctgt catcaggcga cggatattgc ctgcaaattc
     9241 atcatcctga atcagcgaca aatgggcgct gaggatagtt ttgctttcgc cgtcacgctc
     9301 acgcaattgc tggttcagtt gctcggcaag cgttgccagg ctgtgctcca gccgggtgga
     9361 atcttgcgca ctggcgggga ttgcccgata actgtcgagg ctgtcgcttt gtaacagggt
     9421 cagcgtaccc acgccgacgc cgcttgccag cacattgccg tacagtaaat ccgggttaag
     9481 gcggcttaat gaacgcggca gcggatgcgc cgtcagttct gcctgcgtag gctgaacgct
     9541 gtcgctgtcg ataaagcgca cctggatgta ctcttccagc acgcgccgcg cctgctcttc
     9601 atcgctgccg ctaatgttca ggctgcaact gtcattaaat agggtgccgg tgccaatcag
     9661 cgccagcgag cttttggcat cggcctttgc gttctggcga tggttaataa aagtgatttc
     9721 gctttgccac tggctgcact gttctttaag ttcccacgcc ggacgagcat gtagaccgtt
     9781 aggcaaagga cagagaaatt gaatcgttaa cataaccgct ccgggttaat caggagatca
     9841 tctgaaatcc gtcagttgaa caaccgtctc acgcgtaaga cgttgaataa gggcagataa
     9901 aagttgctcc atctgcaaaa tgtcacggca atcggcaatc gacgcggcgc aatgtccgtg
     9961 gcgggtggca ggccccatca ccactgtggg tacgccagta ccggttaagt gcaccgctcc
    10021 accgtccgtg ccgccgttac tgaacatatc cgcctgtaac ggcacgccaa tttctgccgc
    10081 cacggtttcg atccaggcgg tgagttttgg cggcgcaatc agtgacttgt cgcttaacac
    10141 cagcatcggg ccgttaccaa tctggcgatg gttagccgcg ccataattaa agtttttcgc
    10201 ccagcaggcg gtatcaagga caatggcgac gtccggcgac accgcgcggg tggcagtttg
    10261 cccgccgcgt aaccccacct cttcgctgga actggccacc agccacactt ccgcaggcag
    10321 ttcagcgctg tgtagttcgc gcagtaacgt caccagcagg tagcaaccga ggcggtcatc
    10381 aaaggctttc cccatcaccc gctggtgggg gagaacctga aaagtggtat cgaacgtgac
    10441 gcgatcgcct ggacgaattc ccgcctgcat cacttcgtca tacgagcgcg cgccaatatc
    10501 cacgcgcatg gcgctgacat cattcccctg ccggtcgccg tcaagcaggc ctggaatttt
    10561 gcactcttca cgggtggtga tgcgcaccgg ctgcagctgg cgggcagcca tgcgtacatt
    10621 gccaaccggc agcacatcaa tcgccccttc gcgggagatg ctgcgcacca taaatcccac
    10681 ttcgtccata tgcgcacaga tcatcacctt cggacctgtc gattcattga ggcggatcag
    10741 caccgatccc agcccatcaa atcgcacttc tttttgcagg cgatccgctt cttccagcag
    10801 gatctgccgc acttcctgtt ccgaggaggc gatcgcatct gcctcgctca acgctttcaa
    10861 tagcgataaa tccattatgc ctctcccgtg agcaacactg ttttcggcat ggcgtagagc
    10921 acttctgcgc cttgcggggt gaccagcaca acatcctcga tgcgcacgcc cccttgccct
    10981 ggcaaataaa tccccggctc cacggtcagt aacatgcctg gctgtagcgt cgtggtgtcc
    11041 cgcggtgaaa aacgcggatc ttcatgaact tcaatgccga tagcatgagc ggtgttatga
    11101 ccgaaatagt ggctaaaacc tgcctcggta atcacccgac gcgcggcttc gtcaacctgc
    11161 tggcagcgca cgccggggcg aattgcagag attgctgcga gctgtgcctg caaaacaatc
    11221 tgatagacgt taaacagcgg gtgagattcg gcgctcaccc cttcgccatt caccagcaag
    11281 gtgcgcgtca tatcagagca gtagccctga tagagcgcac cgaaatcgag agtgacaaac
    11341 tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg gcagcgcccc acgccagcca
    11401 ctggcgacaa tggtgtcaaa agaggttttt tctgcgccct gctggcgcat aaaccactcc
    11461 agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa taaagcggcg aatatgctct
    11521 gcaccgcgat cggcaatccc acaggcaagg cggattttct ccacctcctc tggcgttttg
    11581 atttgccgca gcacatccgg cgtggcgcta accagtttcg cattgagttc agactgccag
    11641 cgatgcgcgg tttcccaact cacctgctgg ccttcaaaac cgagcgtttg caactgctca
    11701 tcggcaatga tttgcctggc gatggttgta agcgtgtgcg tcgcgtcaag caaatgcagc
    11761 tggtaacctt gcgtgcggac ttctacatcc gcgtaatagc gcgagtccac cagaatgtgc
    11821 gcactttcac ggctaatcag cacatagcct gatccggtgg agatccccag atgcggctgt
    11881 ttgttctgcc gtgaggagag aagcaccgca tccagttgtt gcgccttaag ccagtcgcgc
    11941 agcgaagcga gtaatgtcat ctggattttc cttcttacag gctatcgatt aacaatttgc
    12001 ctttacggaa catcatcaga cgcaggaaca ccaccatcag cgcagttatg actgccccca
    12061 gcgcgatccc cgccatatag acgcccaggt tagtgaccag cggccatgcc cagatagcgg
    12121 attccgggaa ccattgcact gcgcccagcc agacagcggc ggttgagccg acaatcgcgc
    12181 cgaccatata cgacggaatg gcggtgatgg ggctttccag cgcaaacgga atcgcaccct
    12241 cactgatccc cataaaggca aggaacatcg cggtcttacc ctgcggataa agttgtgcgt
    12301 tgaacaggcg tttgccggtt aaacgacggt caataatggt cgccagaccc agaccaatcg
    12361 gcggaataac gatagcgata gaacgagcgg tgaccggtaa tacgtggtca gtggtaaagc
    12421 tgaaggcaac aaaacctgcg gctttgttga tcgggccacc aaggtcgatt gctgtcgccg
    12481 cggcgatccc cattgcgtac atcagcgcgc ccttctcacc tgcggcagtc agcacagtac
    12541 ggataccgcc gttgatccag cccccaaagg gggtgatgac gtagtacatc gccagcatga
    12601 caaaaatagc ggaaagaatc ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg
    12661 gtaactggat tttctggttc atccatttca caaggtaacc ggcaacgata gaaataatca
    12721 gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc ccattgcata gtgctgggat
    12781 cgaagttcag cagttgcgtc ggctgggtag acatcaagcc gccgataaaa ccagccggaa
    12841 acgccagttt gccaccgata gagttcgcca caaaagcagc aaacatcgga atggcaaaac
    12901 caaacaacac gccgccgaag gactgcgaca accaggcaaa tttcagtaag gagaggtcga
    12961 aaccggagaa tttcccgcta ttaagagcat ccatgatgcc gatctcagca ggaattttaa
    13021 gccagctata agcaatcaac tggctgaaag cgaggatcac accgcccata attaaggtcg
    13081 gcaccatgcg tgaaatccct gacatcacat gctgcgggag ttcaccccag aagctgcttt
    13141 tagaggcctg cggattctta actgccgctg ccgcaccgga tgcgccaggc acaacggttg
    13201 cactgcgttt tttaatggcc ataacaatta tccctgtcga atattactgt tgttcagagg
    13261 caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt tttaattgcg tcctgcaaag
    13321 tgatttcata aacgtcgcgt gattcgaaac gttcgttatc ttccggggta actgccacgg
    13381 agtggatgat gatggtcgct tcggcgatat cctgcgccgt caggcgattc tggataccgt
    13441 ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc taccgccgct tcttccagcg
    13501 cctgagcggc cataaaagtg tgagccaggc ccattgggca ggcacataag gcaatcagtt
    13561 tcttactcat cgttaattcc tcgttcagtg atagttgacg agtgaaatta tgaacgtgca
    13621 aaacaaatcg ccgttaccag acaaataatg catttactgg agaattaatc ccgctgtacg
    13681 gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga aaagttgaat taactgtatg
    13741 atttaaaaga ttatcgggag agttacctcc cgatataaca ggaaggattt acagaatgtg
    13801 acctaaggtc tggcgtaaat gtgcaccgga accgagaaga cccggattgt catggacgat
    13861 gagatacacc ggaatatcat ggacatattc tttaaagcgc cctttatctt caaatgcggc
    13921 acggaaacca gaggctttga agaactcaag gaagcgcggc acgataccgc ccgcaataaa
    13981 cacgccgcca aatgtcccga gattgagcgc cagattgccg ccaaaacggc ccataatgac
    14041 gcaaaacagc gacaacgcac ggcggcaatc ggtgcagctg tcagccagcg cgcgttcggt
    14101 aatatctttt ggcttgagat tttctggcag gcggttgtcg gctttaacaa ttgcgcgata
    14161 caaattcacc agtcctgggc cagaaagcac gcgttccgcc gaaacatgac caatttccgc
    14221 acgcaatatt tcgaggataa tggcctcttc ttcactattc ggcgcaaaat caacgtgacc
    14281 gccttcgccc ggcaagctta cccaacgctt atcgacatgg accagatgcg caaccccaag
    14341 ccccgttccg gcaccgtaaa ccgcaatagg cttaccttct accggttctg cgccaccaaa
    14401 ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc gatacagcgg taaaatcgtt
    14461 aataatttcc agatggctaa aaccgagatt ctttttcatt tcggcaattg agaacgccca
    14521 ggtatggttg gtcattgcca cccagtctcc ggtaatcggg caagcgatgg caatacagcc
    14581 gtctttcacc tcgaccttat gttcttcaag ataaacgcga atgaccgctt cgaggctggg
    14641 gtaatcaagc cctgaatagg tcttagcctg cgagatttca ccactggcaa tatcacacag
    14701 agcaagacgt gcgttggtgc cgcccacatc accgactaat gcatactttg tcattcttca
    14761 actgctccgc taaagtcaaa ataattcttt ctcacactgt aaatacctgg gggcataaca
    14821 acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg ggtcacacaa ttactttatc
    14881 gtttcagcac caattgcagc gatgcctttt tgcaagctgg gcaaactaag tatctgaccc
    14941 cgcataagga atagaacatg ctccatccgc gagccagaac catgttgtta ttatcgctcc
    15001 ccgccgtggc aattgggatt gcgtccagtc ttattctgat tgtggtgatg aaaatcgcct
    15061 cggtattaca gaatttgctc tggcaacgac tgccgggaac tctggggata gcccaggatt
    15121 cacccctctg gatcatcggt gtattaacgc taacgggtat tgcggtgggg ttggttatcc
    15181 gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga accgctgatc ggcgcaccgg
    15241 ttccgccctc tgcgctacca ggacttatcg tagcattaat tctcggtctt gctggcggcg
    15301 tcagcctggg gccggaacat ccgatcatga ccgtcaatat cgcccttgcg gttgccattg
    15361 gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac tattttagcc tctgccggaa
    15421 ccatcggcgc gctgtttggt acacctgttg cggcggcgtt gatattttcg caaaccttaa
    15481 atggcagtag tgaagttccg ctatgggatc gtctttttgc gccgttaatg gcggcagcag
    15541 ctggtgcact tactaccgga ttatttttcc atccccattt ttcactgccc attgcgcatt
    15601 acggacagat ggaaatgacc gatattctca gcggtgcaat tgtcgcggcg attgccatcg
    15661 cagcagggat ggtcgccgta tggtgcttac cacggttgca cgcgatgatg catcaaatga
    15721 aaaatccggt gctcgtgctg ggtattggcg gatttattct cggtattctg ggggttattg
    15781 gtggaccagt ttcgctgttt aaagggctgg atgagatgca gcagatggtg gcaaatcagg
    15841 ctttcagcac cagcgattac tttttgctgg cggtaattaa acttgccgcc ctggtcgttg
    15901 ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc agtgtttgtc ggcgtggcat
    15961 tagggttgat gctgcatgag cacgttcccg ccgtaccagc ggcaataacc gtttcctgcg
    16021 ctattctcgg catcgtgctg gtggtaacac gcgatggctg gttaagtctt tttatggcgg
    16081 cagtcgttgt acccaatacc acattgctac cgctgctctg tatcgtcatg cttccggcat
    16141 ggctgttatt agcaggtaag ccgatgatga tggtcaatcg tccgaagcaa cagccacccc
    16201 acgataacgt ttagcaaaaa atgctctcct ttatgattta agagttatgg cgatgatacg
    16261 gggctttacc tccccgtaat attgcgttaa caggccgctg acggcttcga tcgtgaagga
    16321 gaataacaat gttcaggtca ctgtttctgg cggccgccct gatggcattt accccgcttg
    16381 cagcaaacgc aggcgaaatc accctactgc catcaatcaa attacaaatt ggcgatcgcg
    16441 atcattacgg taattactgg gacggtggtc actggcgcga ccgtgactac tggcatcgca
    16501 attatgaatg gcgtaaaaac cgctggtggc gtcatgataa tggctaccat cgtggctggg
    16561 ataagcgtaa agcatatgag cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc
    16621 gcggaaaagg ccgcggacat ggacaccgcc attaaacaca ttcgccaatg gagcacaagg
    16681 cctgatgcga cgctagtgcg tcttatcagg cctacaaacc ggctctttaa cgcgtagggc
    16741 ggataaggcg tttacgccgc atcgccaata gtgccagatg cgacgctcat tcaactacaa
    16801 tcccagcgcc gtccccacca ataaccagat attcagcgcc acgaccagca ctacaatcac
    16861 ccagcctgtc tgttttacgc gtttgctgtt caccagatcg cccatcaact tgctgtcact
    16921 ggtgaaaatc agcagtggaa ccagcgccag ggcgatacca aaacttaaca gcacctgact
    16981 cataaccaga atccgtgtcg gatctaatcc catcagaatg acaataaatg acggcagcat
    17041 ggtgactgta cgacgcaccc acagcgggat atggaagcga atgaacccct gcatcaccac
    17101 ctgccctgcc agtgtcccca ccaccgttga ggacagtccg gcagcaacca gacttaaccc
    17161 aaagaccgtt gccgcagcat ggcttaacag cggttgcagc gtcaaataag cctcatcaag
    17221 atcggcaaca ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc
    17281 cagattgaca aaaccggcaa tagtcatggc gatagccaca tcccatttgg tggcggaata
    17341 acgttgttga cgcgaaccgc catgtaaatg ctgagtgagc gaagagtgca aataaatcac
    17401 atgcggcata atcgtcgccc ctaacacgcc tgctgccagg aagaccgcct ccgaagtagg
    17461 taaactcggg atcaccattc ctttacccag ctgcgccagg ttaggctggg agaaaatcaa
    17521 ctcgacaatg taagccgcgg caacaaacaa cagtaacccg ccaatcactt tctccagcgg
    17581 tttttgcccg cgacgttgca gcattaaaat caggaaagtc gcgatccccg tcagcaccgc
    17641 gccctgcaac aacgaaacac caagaatgag tttaaaaccg atcgccgcac caataaattc
    17701 cgccaggtcg gttgccatcg caataatttc tgcctgaacc caatagaacc acactacggg
    17761 acgcggatag tgatcgcgaa tctgctccgc cagattttta ccggtggcaa tccctagttt
    17821 ggcagagagg atctgaatca gcatcgccat caggttggcc caaacgacaa cccacagtag
    17881 ctgatagccg aagctggcac ccgcctgaat attggtcgca aagttaccgg gatcgatata
    17941 accaatcgcc gcaatgaacg caggtcccat taatgcgagc ctcatcttgc gcgccgcccg
    18001 tccgctgcta ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca tagcctttgc
    18061 tatgtttcat gctatgccaa acgagaatga ttatcaaatt catttaaatg ggttgtggtg
    18121 atttctctga tagaccagga ttatgactac gaaaagattg atgacttgaa tgttatggtt
    18181 atgtttaatg ttagcacatt tacataactt tcagcttcca tacacaacat agcagaaatg
    18241 tatgacagct cactattttt gaagcttgtc acaggacgtc attatagtgt gtgtcagatc
    18301 tcgttttcct taaccatgtt acatagaatg tgcacggaaa tttaacctgc ctcatatttg
    18361 gagcaaatat ggaccgcgtc cttcattttg tactggcact tgccgttgtt gcgatcctcg
    18421 cactgctggt aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg
    18481 ttatcgaagt gttactggcg tggttcttcc tgaactccga cgttggttta ggcttcgtga
    18541 aaggcttctc cgaaatgttc gaaaaactgc tcggatttgc caacgaaggg actaacttcg
    18601 tctttggtag catgaatgat caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa
    18661 tcgtctttat ctctgcactg atcggtattc tccagcacat tcgcgtgttg ccggtgatta
    18721 tccgcgcaat tggtttcctg ctctccaaag tcaacggcat gggcaaactg gaatccttta
    18781 acgccgtcag ctccctgatt ctgggtcagt ctgaaaactt tattgcctat aaagatatcc
    18841 ttggcaaaat ctcccgtaac cgtatgtaca ccatggcagc aacggcgatg tccaccgttt
    18901 ctatgtccat cgttggtgca tacatgacca tgctggaacc aaaatacgtc gttgcagcgc
    18961 tggtactgaa catgttcagc acctttatcg tgctgtcgct gatcaaccct taccgtgttg
    19021 atgcctgtga agaaaacatt cagatgtcca acctgcacga aggtcagagc ttcttcgaaa
    19081 tgctgggtga atacattctg gcaggtttca aagttgccat tatcgttgcc gcaatgctga
    19141 ttggctttat cgccctgatc gccgcgctga acgcactgtt tgctaccgtg actggctgga
    19201 ttggctacag catctccttc cagggcatcc tgggctacat cttctatccg attgcatggg
    19261 tgatgggtgt tccttccagt gaagcactgc aagtgggcag tatcatggcg accaaactgg
    19321 tttccaacga gttcgttgcg atgatggatc ttcagaaaat tgcctctaca ctctctccgc
    19381 gtgctgaagg catcatctct gtgttcctgg tttccttcgc taacttctct tcaatcggga
    19441 ttatcgcagg tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg
    19501 gtctgaagct ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac
    19561 tggtgctgta agaccataca taaaaaagcc ggggataatt ccccggcttt tttacgcctg
    19621 ttaatcagct aatggctgcg ggcgaccaat caaataccct tgcagatatt gcaccccgag
    19681 cttatgcaat agcgcctgtt gctgttgcgt ctcgacaaac tccgcgacca cactcaatga
    19741 cttcgctttc gccagatcgg taattgatct cacaatcatc gcatccagcg tattcgtgac
    19801 aatatctttc acaaagacgc catcaatttt gatgatatca gcctgcaaac gctttaaccg
    19861 ttcgtagttg gcatagccgg tgccaaagtc atcaatcgca atccggaaac caaacttatg
    19921 cagctgctcg atgttgtaca tgctgctttc tgcgttagaa aacgcctgct cctcggtgat
    19981 ctcaagaatg accgcctgcg gggagatgtg ataacgctta aatagacgaa taatccgccc
    20041 ggcaatattc ttttgcagca gcgtgagcgg cattaaatta actgaaaagc gcgggccttt
    20101 tttgtcgcaa gggtgtgttg ccagccactt caacaaggat tccagcactt gcaaatcaaa
    20161 acgcgcgcta aggttaaact gagcaataag gggcagaaac ttatccgggg tcataatgcc
    20221 gccgtcatat ttcagtcgcg cgaggacctc atcataacct tcaccctctt tgttgcgaat
    20281 tggctgggcg tagagcagca aatcaccctg atctaacgcc gtgcgaatgg tattcagcaa
    20341 tagcacctgt ttagtggtct gcccggaaac catctcctct cggctatcca gcgccagcac
    20401 atgatgatgt gcgcaggatt gctccgccag ccagcttaac tgccccaaca agggttgcag
    20461 ggtttcctga tttccatcaa aacgccccca ggcagcacca tagcccatat ccagcccggt
    20521 attgttccag tgaatttgcc gactattcag gatgttaacc atatgctgga gtcgcccttc
    20581 cgtttcaggc ccgctcagca ctaacagcag ttcactaccc ggcaattgat acaacttttc
    20641 gttttcctgc atcaatggca gcagcgtacg gtaaattgat cggatacagt gaacacgcat
    20701 catcaacccg taatgacgac tcataaactc aagattatca atgcgcaggc agcaaaaact
    20761 cttgcccgcc tcttgctccg gcgcttgttc caacgcacga aagttgggca ggagcgttaa
    20821 cggatcggtc agcgcctgca aatgccagcg gcgattaagc cattcactgc gatgataaat
    20881 gcgcaccata tagagcaggc aaacgctaaa ggaaatcagc accgcgagaa taaatgccag
    20941 cgaatattcg gtttccaccc cttgcaaaaa gttctggttg taattcagca ggcaaagcgt
    21001 tgaaacagcc caagtgagat ttaaaaacgg atagcgaagc ttaccgaccc cgagggtaaa
    21061 gatgatgaag aaaacgggta ccaggtaacc ggcaataaag tcgttttcat aaggtgtgca
    21121 caacagaagt agcagcacgc ttaacgctgc tagccagctt aaggtaaatg cgcgtttctc
    21181 tttgcccaac gacggagcga tatccctgcg ccacaatatc tgcgcaaagt ggggacttac
    21241 aatcatgcga gtgagatagt agaaaagcat gttgtaaatc agcacagcgg tgaaaaggct
    21301 tagcaaatca acgaccgtga aaatggcatc cgcatcgccg aaaaaggtgg atatctttag
    21361 cgggaaatca aagaagctcc ccacaagata catgctgcat ttgatgccga tcggcgtcac
    21421 caaaccaagc cagaacagac gttgccagac atatcggctg gtcaatccat aacgccaacg
    21481 cgtccccagc tgccagcgta atattgcgca ggcacacaac acgacaaacg tctggctacc
    21541 gagcatgacc gcagtctgta aaaaagagag atgaaagttc cacagattgg tgcaaaacat
    21601 cccgcataaa acgggaacga caccgcgcca gccaaagata aacaagattg acagcattac
    21661 gcacagtggc atccacgcca gaaagatata actggaatgg ataatggcaa gtggcgaaat
    21721 aaaacgggat agctgaataa gtaccacggt gagcgtaaac gctagtgtga atatcttgat
    21781 attttttatc aggttatgct ccacaaacat gagtacacta ctctcactga tcggggtttt
    21841 caggcacaca aataataagt ttattcgcat tgcgaagcaa acaggggcca tcctgttgcc
    21901 gatttttcat ccaatgatgc tttcaccagg cggcactaac ccggacgtag actgtattac
    21961 aaaagcggca aaaagcagag acaaaaaacc cccgctttgc agcgagggtt ggaaatttgg
    22021 tggagctaag cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct
    22081 gagctatagc cccacgatgc gtttacgtac caaatttgct gggtgcaaaa tttggtggag
    22141 ctaagcggga tcgaaccgct gacctcttgc atgccatgca agcgctctcc cagctgagct
    22201 atagccccgt cacgtaaagc ttgtcgagtt gacgggcggc atcatatgaa ttccgcccga
    22261 atgtgtcaac ggcaaattgc aacgtgtagt ttcaatcgct gaaaaatcag gcaaatgaac
    22321 aattttggaa aaccgctcgc attcagtagt tattcatgtc acggtttcct gtaaagtggt
    22381 gctataaaat gaactactaa tagacccaca tacattcagg gaattgttat gttcaaggag
    22441 aggatgacac cagatgaact tgccagactg accggctata gccgccagac cattaataaa
    22501 tgggtacgca aggaaggctg gacaacgtca ccaaaacctg gcgtccaggg tggcaaagcc
    22561 cgactggttc acgtcaatga acaggttcgt gaatatattc gtaatgctga acgtccagaa
    22621 ggtcagggag aagcgcctgc cctttctggt gatgcgccgc ttgaagtatt gctggtgaca
    22681 ctggcgaaag agatgacgcc agttgaacaa aaacagttta catccttgct tctgcgggaa
    22741 gggattatcg gattgttaca acgcttaggg attcgcgata gcaaataata tgaaaagatt
    22801 acgcaataaa atgaccaccg aagaactggc tgaatgcctc ggtgtggcta aacaaactgt
    22861 taaccgttgg atcagagaaa aaggctggaa aacggaaaaa tttcccggcg tgaaaggcgg
    22921 tcgtgccaga cttattctgg tcgatacgca agtttgcgag tttattcaga acacgccagc
    22981 cttccataac acaccgatgc ttctggaagc cgaagaaccc cttgccgaat acgcaccggg
    23041 gattcgcact cctgcttatc ggcaaatcat cagtgcaata gacaacatga ctcacagtga
    23101 gcaggaaaaa gttgcgcaat ttttgtcacg cgaaggaatt cgtaatttcc ttgctcgtct
    23161 cgacatagat gaatcagctt gacatcctcc acgccctgaa tgacgaggat ccctgctacg
    23221 ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca
    23281 ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccgttta
    23341 catcggcgtt cgctgtatat ccacatacct ggcatctgaa tttactttgt gacaggcggt
    23401 tttctttcgc tgtatgacca cagcacgcgc aacgctggct tgtgtacgct ggcggaacag
    23461 ccagtacctg accgccacgc cagagctgct tgtactcaag ctggcggcgc atttcat
//
LOCUS       DAZFDC010000071        23045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFDC010000071 DAZFDC010000000
VERSION     DAZFDC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.071"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            77..1330
                     /gene="ynfM"
                     /locus_tag="ACHJ48_002864"
     CDS             77..1330
                     /gene="ynfM"
                     /locus_tag="ACHJ48_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4708381.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1379..1498)
                     /locus_tag="ACHJ48_002865"
     CDS             complement(1379..1498)
                     /locus_tag="ACHJ48_002865"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO4708382.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            1754..2062
                     /gene="asr"
                     /locus_tag="ACHJ48_002866"
     CDS             1754..2062
                     /gene="asr"
                     /locus_tag="ACHJ48_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO4708383.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            2155..2238
                     /locus_tag="ACHJ48_002867"
     CDS             2155..2238
                     /locus_tag="ACHJ48_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708384.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2338..3159
                     /gene="ydgD"
                     /locus_tag="ACHJ48_002868"
     CDS             2338..3159
                     /gene="ydgD"
                     /locus_tag="ACHJ48_002868"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO4708385.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3198..3527)
                     /gene="mdtI"
                     /locus_tag="ACHJ48_002869"
     CDS             complement(3198..3527)
                     /gene="mdtI"
                     /locus_tag="ACHJ48_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO4708386.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3514..3879)
                     /gene="mdtJ"
                     /locus_tag="ACHJ48_002870"
     CDS             complement(3514..3879)
                     /gene="mdtJ"
                     /locus_tag="ACHJ48_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO4708387.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4291..5325
                     /gene="tqsA"
                     /locus_tag="ACHJ48_002871"
     CDS             4291..5325
                     /gene="tqsA"
                     /locus_tag="ACHJ48_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO4708388.1"
                     /translation="MAKPLITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSTLNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFKQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5350..6738)
                     /gene="pntB"
                     /locus_tag="ACHJ48_002872"
     CDS             complement(5350..6738)
                     /gene="pntB"
                     /locus_tag="ACHJ48_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO4708389.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6749..8281)
                     /gene="pntA"
                     /locus_tag="ACHJ48_002873"
     CDS             complement(6749..8281)
                     /gene="pntA"
                     /locus_tag="ACHJ48_002873"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO4708390.1"
                     /translation="MRIGIPRERLTNEPRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGCFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKNGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQVAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8805..9749
                     /gene="ydgH"
                     /locus_tag="ACHJ48_002874"
     CDS             8805..9749
                     /gene="ydgH"
                     /locus_tag="ACHJ48_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO4708391.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9935..11317
                     /gene="ydgI"
                     /locus_tag="ACHJ48_002875"
     CDS             9935..11317
                     /gene="ydgI"
                     /locus_tag="ACHJ48_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4708392.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDIFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11354..12076
                     /gene="folM"
                     /locus_tag="ACHJ48_002876"
     CDS             11354..12076
                     /gene="folM"
                     /locus_tag="ACHJ48_002876"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO4708393.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12073..12408)
                     /gene="ydgC"
                     /locus_tag="ACHJ48_002877"
     CDS             complement(12073..12408)
                     /gene="ydgC"
                     /locus_tag="ACHJ48_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO4708394.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            12537..13256
                     /gene="rstA"
                     /locus_tag="ACHJ48_002878"
     CDS             12537..13256
                     /gene="rstA"
                     /locus_tag="ACHJ48_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO4708395.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDIQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHTLALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13260..14561
                     /gene="rstB"
                     /locus_tag="ACHJ48_002879"
     CDS             13260..14561
                     /gene="rstB"
                     /locus_tag="ACHJ48_002879"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO4708396.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            14637..15566
                     /gene="tus"
                     /locus_tag="ACHJ48_002880"
     CDS             14637..15566
                     /gene="tus"
                     /locus_tag="ACHJ48_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO4708397.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSQALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15563..16966)
                     /gene="fumC"
                     /locus_tag="ACHJ48_002881"
     CDS             complement(15563..16966)
                     /gene="fumC"
                     /locus_tag="ACHJ48_002881"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO4708398.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(17109..18755)
                     /gene="fumB"
                     /locus_tag="ACHJ48_002882"
     CDS             complement(17109..18755)
                     /gene="fumB"
                     /locus_tag="ACHJ48_002882"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO4708399.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSLSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQALGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18954..20129
                     /gene="manA"
                     /locus_tag="ACHJ48_002883"
     CDS             18954..20129
                     /gene="manA"
                     /locus_tag="ACHJ48_002883"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO4708400.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20230..21738
                     /gene="ydgA"
                     /locus_tag="ACHJ48_002884"
     CDS             20230..21738
                     /gene="ydgA"
                     /locus_tag="ACHJ48_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO4708401.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(21783..23045)
                     /gene="uidC"
                     /locus_tag="ACHJ48_002885"
                     /pseudo
     CDS             complement(21783..23045)
                     /gene="uidC"
                     /locus_tag="ACHJ48_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
BASE COUNT         5698 a         5932 c         5567 g         5848 t
ORIGIN      
        1 atattagaca gaacaattcg attttcctaa cctatgtata agcctgatcc acaggcatat
       61 ttagcaagga tttcaagtga gccgtactac aactgttgat ggcgctccgg caagcgacac
      121 tgacaagcaa agcatttctc agccaaatca atttattaaa cgcggtacgc cgcaatttat
      181 gcgcgtcacc ctggcgctgt tctctgccgg actggcaaca tttgcacttc tctattgtgt
      241 gcaacctatc cttccggtgc tttcgcagga gtttggctta acgcccgcga acagtagtat
      301 ttcactgtcc atttccacgg cgatgttggc tattggtttg ctgtttactg gccccctatc
      361 cgatgccatt ggtcgcaaac cagtgatggt aacggcgcta ctattggcct ccatttgtac
      421 gttactttcg acaatgatga ccagctggca cggcattttg attatgcgcg ccttgattgg
      481 gctttcgtta agtggcgtgg cagctgttgg catgacttat cttagcgagg aaatccaccc
      541 cagtttcgtg gccttttcga tggggttgta tatcagcggc aactcaattg gcggcatgag
      601 cggacgctta attagtggtg tcttcacgga ctttttcaac tggcgaattg ctctggcggc
      661 tatcggttgt ttcgcgctag cctcggcgtt gatgttctgg aaaatcctcc cggaatcacg
      721 ccattttcgc ccgacttcgc tgcgacctaa gtcgttgttt atcaactttc gtctgcactg
      781 gcgtgaccgg ggattaccgt tattgttcgc agaaggcttt ttgctgatgg ggtcgttcgt
      841 cacgctgttt aattacatcg gctatcggtt gatgctctcc ccctggcatg tcagtcaggc
      901 cgtggttggc ttattatcgc tggcttattt gaccggtaca tggagctcac ccaaagccgg
      961 aaccatgacc acccgctatg ggcgtggtcc agtgatgttg ttttcgacgg gggttatgct
     1021 gtttggttta ctgatgacct tattcagctc gctgtggctg atctttgccg gaatgttact
     1081 cttctcagca ggattcttcg cagcccactc agttgccagc agctggatcg gcccccgcgc
     1141 aaaacgcgct aaaggccagg cctcctcgct gtatctgttc agttactatc tggggtcgag
     1201 tattgccggg acgctgggtg gtgttttctg gcataactat ggctggaacg gcgtcggcgc
     1261 atttattgct ctgatgctgg tcattgctct gctggtcggg acgcgtttgc atcgtcgtct
     1321 gcacgcctga aaaataagtc cggactgcgg taaatacccg tccggactta ttgcctgctc
     1381 aaaccaacgt taatagccat cctaaaatag acgaagcgcc agccaattcc cgcagcgcgt
     1441 ctagcgtcat caggattata agtacccaaa taaacggatt cattttgctg tgtgtcattt
     1501 attactgatg cgcagttatt ctaccgcttt gtaagtagta aaatagttaa cccgatcaag
     1561 actactatta ttggtagcta aattttcctt aagtcacaat acgttattat caacgctgta
     1621 atttattcag cgtttgtaca tatcgttaca cgctgaaacc aaccactcac ggaagtctgc
     1681 cattcccagg gatatagttc tttcaacggc cccgcagtgg ggttaaatga aaaaacaaat
     1741 tgagggtatg acaatgaaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc
     1801 ttctgccgcc tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa
     1861 agcagcgccg gcgaaaacta cacatcataa aaaacagcat aaagcagcac ctgcccagaa
     1921 agcgcaggcg gctaaaaagc atcataaaaa tacgaaagct gaacagaaag ccccagaaca
     1981 aaaagcgcag gcagcgaaga aacacgccaa gaaacacagc catcagcaac cggcaaaacc
     2041 tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg
     2101 aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg tagtatggtg
     2161 ggccgttatc gctttgagtt cattcttatc atccttattt tatgcgcact gattgccgcc
     2221 cgtttttatc tttcctgatc gtagttatct gattttactc ccactttcat cccgtcccgt
     2281 ctatagtatt tatgagggtt tgcttttaat aatcataatt acccaccaga gtgtgatatg
     2341 cgtacaacca ttgctgtagt gttgggtgca attagtttga cgtctgcttt tgtgtttgca
     2401 gataaaccag acgttgccaa atcggcaaac gatgaggtca gcaccctgtt ttttggtcat
     2461 gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt gggatgcggt tgggcaactg
     2521 gaaacggcca gcggcaattt atgtacggcg acgctgattg cacccaatct ggcattaacg
     2581 gcaggacact gtttattgac gccaccaaag ggtaaagccg ataaagcagt ggcgctgcgt
     2641 tttgtgtcaa ataaaggtct ttggcgctat gacatccacg acatagaagg ccgcgttgat
     2701 ccgacactgg gaaagcggtt aaaagcagat ggggatggtt ggattgtacc tcccgcagcc
     2761 gcgccgtggg acttcggatt gattgtgcta cgtaatcccc cttctggcat tacgccgttg
     2821 ccgttatttg agggagataa agccgcgctt accgccgcat taaaagcggc aggtcgtaaa
     2881 gtgactcagg caggataccc tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt
     2941 gaagtgactg gctgggcgca aacgtcggtg atgtcacatc agtgcgatac cttgccgggt
     3001 gacagcggtt cgcctctgat gttgcatacc gatgacggct ggcaattaat tggggtgcaa
     3061 agttcggctc ctgccgcgaa agatcgctgg cgcgccgata accgggccat ttctgttacc
     3121 ggttttcgcg acaagctgga tcaactgtcg caaaaataat gttcaaacgc tgcccgacag
     3181 cgcgggcagc gtcttcatca ggcaagtttc accatgatca ttccagccaa cagcaagacc
     3241 aggccaatcc agcctttacg atttaaccgt tgaccaaaca agatccaacc tgcggctaac
     3301 gtggcggcaa taccaaaccc gccccacaat gcataagcga cagacaagtc gatcccttta
     3361 acggcctgag aaagcgcact aaaggcagcc agcaccgccg ccagggagag caagccaaat
     3421 attttgcgac gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat ttccagcacg
     3481 attgccaatg ccagccaggc ggcgtgaacc cattcaaact gcgccatggt tcacctccag
     3541 ttcaggttta cgtgctttac gggtacctga tttgatcaac actatcccgg cgaccagggt
     3601 ggttaacccg gcaattttca tcagcgataa actttcgtcg aataacaaaa cgctaaacaa
     3661 ggtaataaat aaaataccga taccttccca cagcgcataa gccacaccta aggctatttt
     3721 tttaacagcg aaagagagaa atatatacga cagagaaatc atcaccagca ttaaaataaa
     3781 gccgccattt ccctcactga cgctcgccca tttcatagac agcgtaccgg taatttctgt
     3841 agcaatagcc agacctaata aaatccaata aatatacatt gtccttctcc tgcaagagaa
     3901 ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgtata
     3961 gggcaagctt aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga
     4021 gcaagatagt ggatgaggta cgaagatggg taaatgtaga aaataacgtc ctgaacaaat
     4081 tgtccataat attacaatta tccgcagtgt tgcttctcgt catcgcggat gataattgtc
     4141 ctcggtagtt gaacacgcct gatttgtatc atagcttaag aattaactca aaatattttc
     4201 acttctttac ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa gacattatta
     4261 ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc cgctcatcac gctcaatggc
     4321 ctaaaaatcg tcattatgtt gggaatgctg gtcattattc tctgcggtat ccgttttgcc
     4381 gccgagatca tcgtgccgtt tattctcgca ttatttattg ctgttattct taacccgctg
     4441 gtgcaacaca tggtccgctg gcgtgtgccg cgtgtactgg cagtgtcgat tttgatgacc
     4501 atcatcgtga tggctatggt gttgctgtta gcttatctgg gttccacgct caacgagttg
     4561 acgcggacgt taccgcaata tcgcaactct attatgacgc cgctgcaagc tcttgagccg
     4621 ttgttgcaac gcgtagggat tgacgtctca gttgaccaac tggcgcatta cattgatccg
     4681 aacgcggcga tgacgttgct caccaactta ttgacgcagt tatctaatgc catgtcatca
     4741 atatttttat tgctgctgac ggtgctgttt atgctgctcg aagtgccaca attgcccgga
     4801 aaatttaagc aaatgatggc gcgtccggtt gaagggatgg cggcgattca gcgtgcgatt
     4861 gacagtgtgt ctcattatct ggtgctgaaa acagccatca gcatcatcac cggtctggtc
     4921 gcatgggcga tgctcgccgc cctcgatgtt cgcttcgctt ttgtctgggg attgctggcc
     4981 tttgcgctta attacatccc taatattggt tcagttctcg cggcaatccc ccctattgct
     5041 caggtactgg tgtttaatgg cttctacgaa gcgttgttgg tgctggcggg atatctgctg
     5101 attaatctgg tcttcggcaa tattctggag ccacgcatca tggggcgtgg gctgggactt
     5161 tccacattgg tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt
     5221 atgctgcttt ccgtgccgtt aacaattatt gtcaaaattg cgcttgaaca aacagcggga
     5281 ggtcaaagca tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg
     5341 ccgtcagggt tacagagctt tcaggattgc atccacgctg gctttggcgt caccaaacag
     5401 catgtgggtg ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat
     5461 cgaacgttta aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc
     5521 aatcggactc ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac
     5581 cagtacggta tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc
     5641 atacggtact tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac
     5701 cgggtggata ccgaaacgca cgttgatacc acgagcgcgc agtttctcgg taatttcagc
     5761 gacaggatat tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg
     5821 ggagtttttc aacagttccg ctgtctcttc tgcggtgatt tcgcggtgct cacccacttc
     5881 ctgatcatcg ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa
     5941 ggaacggttc atcgccttac acataatgta agaaaggata gcccccgagg aaccgaccag
     6001 cgcaccggtc acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca
     6061 gccggagtac gagttcagca tcgacaccac caccggcata tctgcaccac cgatggaggc
     6121 gactaaatgc cagccaaata ccagcgcaat ggcggtcatt attagcaatg ccagcacttg
     6181 caggccgacg ctatccgtgc gaacaaatac aatcagcagc aggaaggaaa cgaccagagc
     6241 tgccaggttc attttgtgac ggtttggcag catcaacggt ttagacgaaa ttttgccaca
     6301 cagtttgccg aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag
     6361 aaacacttcc gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag
     6421 atagctgtta aagccaacca gcactgccgc cagacccacg aagctatgca ggatcgccac
     6481 cagttctggc atttcggtca tttcaacttt cttcgccaga cggataccaa ttgccccacc
     6541 aatgaccatc gccagcaaga tccagccaac gttacccgta tccggtccaa aaatggtggc
     6601 gattagcgca atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg
     6661 tttcgaaagc ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt
     6721 aactaatcct ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct
     6781 gagtcacggt gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa
     6841 ggaagctaac ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg
     6901 aaatcgcgtt ggtgaccgac atcaacggtg tatgcagcgc gtgcgatacg ttccacacca
     6961 cgtaataacc gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg
     7021 gcgcaacgct tgccagccag ccgaaaagga tgattgccag cgccatcaac gcgtatttac
     7081 gccacggtga acaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgcca
     7141 cctgcggctg agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga
     7201 tcacagtcac gccgcgaatc accacatcat caaaatcaac agtgatgtta ccgtttttct
     7261 ctttgcacaa cagtttcagc agattaacga ggttagtgcc gtaaagctgt gaggattgcg
     7321 tcggcagacg gcccggaaga tcggtatagc caatcacttt gacaccattt tccgtagtga
     7381 agatttcacc cggcacggtg tattcacagt tgccgccgtt ttgggctgcc aggtcgacaa
     7441 tcacactgcc cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt
     7501 tgcctggaat aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga
     7561 gttccatttc cgctttgatg aacgcgtccg acatcacttt ggcatagcca tcgccgctgc
     7621 ccgcttcctc tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt
     7681 tcacttccgg gcgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa
     7741 tggcggccag acctgcaaca cccgcaccaa tcaccatcac ttttgccggt ggcactttcc
     7801 cggccgcagt aatttgcccg gtaaagaagc acccaaattc atgtgccgct tcaacaatgg
     7861 cgcgataacc ggcgatattc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga
     7921 tacgcggcac agagtccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta
     7981 attccggatt ctgcgcaggc cagataaaac tcaccagcgt tgtcccagga ttcagtaacg
     8041 caatttcatc atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat
     8101 tcccttctac aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt
     8161 gacccgcgcc gctctctacc gcgacggtaa accccagttt cagcagctgt tccactgttt
     8221 ttggcgttgc tgcaacacgg ggttcattgg ttaaccgttc tcttggtatg ccaattcgca
     8281 tgatattccc ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag
     8341 ccgctcatgt acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata
     8401 acgtactgaa aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg
     8461 caaaatatta acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga
     8521 ctgataattg tcttcgacgg tcgggtaaaa cgagacatcg ccccggcacg aatcactact
     8581 taacattaaa ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg
     8641 ttttttaagc atagacggtc atttgagcag gattaaaatt ggcttaagga atgtgatatg
     8701 aaaaatgacg cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat
     8761 aacaatacca gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca
     8821 ccctcctggc gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac
     8881 tgacaccgga gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt
     8941 ttaatgctat tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg
     9001 cctcttttta tgttgtcgac acttctgatt ttgggaacag cggtaactgg cgtgtggtcg
     9061 ctgacctcta taaagccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg
     9121 gtgttgtcga actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg
     9181 tccagggctt ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag
     9241 cgaaaggtgc ctactctttc tacatcgttc gtcaaatcga tgccaaccag ggcggcaacc
     9301 agcgtattac tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg
     9361 atgtgatccc ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg
     9421 cgaagaaagt tgagatcccg ggtgttgcga ctaccgcatc accaagttct gaagtcggtc
     9481 gcttctttga aacccagtca tcaaaaggcg ggcgttacac tgtcacgctt ccggatggca
     9541 ctaaagtcga agaactgaac aaagcgacag cagcgatgat ggtcccgttc gacagcatca
     9601 aattctctgg caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgagccg
     9661 cgaagaaagg tgctaaatat taccacatca cccgccagtg gcaggaacgt ggtaataacc
     9721 tgaccgtcag tgcagatctg tataaataac tattcagtta ctcttataag gcggcttgat
     9781 gccgcctttc gcatttttag tcacaaattt ccccgcgacc attgcatgca gacacatctc
     9841 ttcgtaaaat cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt
     9901 attcttctta tcacgtttta atcactggat atcgatggaa aagaaactgg gactgagcgc
     9961 actcaccgcg ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa
    10021 tatggcggca gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg
    10081 cattttattg ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgacgg
    10141 cggtatcttt acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg
    10201 gggatactgg ctgtgcgcag tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc
    10261 gttaagcttt tttacggaca cgccggaatt gcgcctgttt ggcgatggta atacctggca
    10321 atcgatagtc ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt
    10381 gcaaaccgct gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct
    10441 gtttgttgtg ctggcgatga tgatgttcaa actggatatc ttcaagctcg acttcaccgg
    10501 acttgcactt ggcgtacctg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg
    10561 ggttttcatt ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga
    10621 tgttggcaaa gcgacactgc tggcggttct ctccgctctg ggcgtttact tgctggtaac
    10681 gctgctttcg ctgggagtgg tggcacgtcc tgaactggct gaaattcgta acccgtcaat
    10741 ggccggtctg atggtggaaa tgatgggacc gtggggcgaa atcatcatcg ctgccggttt
    10801 gatcgtttcc gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt
    10861 cctggctgcc actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc
    10921 gccatccgcc tcactgtggt tgacaaacat ctgcgtgcaa atttgtctgg tactcatctg
    10981 gctgaccggt tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc
    11041 ctatttcctg gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt
    11101 aggtgtcggt gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt
    11161 gctgttatcc gttgttctgt atgcaccggg gctgctggtt ttcctgtatg cgcgcaaaac
    11221 gcatacacat gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat
    11281 tgcctcagtt ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagc
    11341 aaaggagata acgatgggta aaacccagcc cttgccaata ttaattactg gcggaggtcg
    11401 tcgcatcggc ctcgccctcg catggcattt cattaatcaa aagcaaccgg tgattgtcag
    11461 ctatcggaca cactatccag ccattgatgg actgattaat gcaggtgcgc agtgtattca
    11521 ggctgatttt tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaagcac
    11581 ccatggtctg cgtgctattt tgcataacgc cagtgcgtgg atggcggaaa aaccgggtgc
    11641 gccactggcc gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct
    11701 caaccatgcg ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca
    11761 ctttaccgat tatgtggtgg agcgcggtag cgacaaacat attgcgtatg ctgcaagcaa
    11821 agcggcactg gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt
    11881 gaattctatt gcgccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca
    11941 acaggcgctg aataaatcat tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct
    12001 ggtcgattac ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg
    12061 tcgtcatctg cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc
    12121 ccaacacgca acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca
    12181 cagcgacacc aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc
    12241 gcgtaaggct tcaatgccgc gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt
    12301 cgggaaaagt ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat
    12361 caacagcact accagcgcac cgagcgcggc tttgattacc agccccatct ttttacctta
    12421 acacttccat aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga
    12481 tagtaagtaa aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga
    12541 acactatcgt atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctgg
    12601 caaaacatga tatacaggtt accgtagagc cgcgcggcga ccaggccgaa gaaaccattt
    12661 tgcgagaaaa tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga
    12721 ccatttgtcg tgatttacgc gcaaagtggt ccggaccgat tgttcttcta acctctctcg
    12781 atagcgatat gaaccacacc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa
    12841 cgacgccccc tgctgttttg ttagcgcgtt tacgtttgca tttgcgtcag aatgagcaag
    12901 ccacgctgac caaaggtctt caggaaacgt cactgactcc ctacaaagcc ctgcatttcg
    12961 gcacgttgac catcgatcct atcaaccgcg tagtcaccct ggctaacact gaaatctcgc
    13021 tctcgacagc tgatttcgaa ttattgtggg aattagctac ccatgccggg caaatcatgg
    13081 accgcgatgc attgctgaaa aatttacgcg gcgtcagtta tgacggcctg gatcgtagcg
    13141 tggacgtggc tatttcgcgg ttaagaaaaa aactgctcga taacgccgct gaaccttatc
    13201 gcattaaaac tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg gaataagcga
    13261 tgaaaaaact gtttatccag ttttacctgt tattgtttgt ctgcttcctt gtgatgtctc
    13321 tgctggttgg gttggtgtac aaatttaccg ccgaacgcgc gggcaaacag tcgctggatg
    13381 atttgatgaa cagttcgctg tatctgatgc gcagcgaatt gcgtgagatc cccccacatg
    13441 actggggtaa gacgctgaaa gagatggatt taaatctctc tttcgatctg cgagtcgagc
    13501 cactgagtaa ataccatctt gatgatattt ccatgcaccg gctgcgtggc ggcgaaattg
    13561 tcgccctgga cgatcagtac acttttttgc agcgtatccc gcgcagccac tacgtgctgg
    13621 cagttggtcc tgttccttat ctttattatc tccatcagat gcgattgctg gatatcgccc
    13681 tgatcgcttt tattgctatt tccctcgcct ttccggtgtt tatctggatg cgtccgcact
    13741 ggcaggatat gttaaaactg gaagcagcgg cgcaacgatt tggcgatggg catctcaatg
    13801 aacgtatcca ctttgatgag ggttcgagct ttgaacgact tggtatcgca tttaaccaga
    13861 tggcggacaa tatcaacgcc ttgattgcca gcaaaaaaca gcttattgac ggtatcgctc
    13921 acgaactgcg aacaccgtta gtgcgcctgc gttatcgact ggagatgagc gataacctga
    13981 gcgccgccga atcccaggcg ttgaatcgtg atatcagtca acttgaagct ttaattgaag
    14041 agctgctgac ttatgcccga ctcgatcgcc cacaaaacga gcttcatctt agcgaaccag
    14101 acctgccgtt gtggctgtca acgcatctgg cagatattca ggcagtaacg cccgataaaa
    14161 cggtacggat aaaaacgctc atgcaaggcc attatgcggc gttggatatg cgcttaatgg
    14221 agcgcgtgct ggataatttg ctcaataatg ccctgcgcta ctgccattca acggttgaaa
    14281 ccagcctgct actgtcgggg aatagagcga cattaattgt tgaggatgat ggcccaggga
    14341 ttgccccaga aaaccgcgaa catatctttg aaccttttgt tcgcctcgat cccagccggg
    14401 atcgctcaac cggcggctgc gggctggggc tggcaattgt ccactctata gcactggcaa
    14461 tgggcggtac ggttaattgt gacaccagcg aactgggtgg tgcccgcttc tcgtttagct
    14521 ggccgttatg gcataacatc ccgcaattta cctctgcttg acactacgcg cacggtggtc
    14581 aagtcaccac cactgtgcta taaattaagg atgttgtaac taaagtggtt aatattatgg
    14641 cgcgttacga tctcgtagac cgactcaaca ctacttttcg ccagatggaa caagagctgg
    14701 ctgcatttgc cgctcatctt gagcaacaca agctattggt tgcccgcgtg ttctctttgc
    14761 cggaggtaaa aaaagaggat gagcataatc cgcttaatcg tattgaggtg aaacaacatc
    14821 tcggcaacga cgcgcagtcg caggcgttgc gtcatttccg tcatttattt attcaacaac
    14881 agtccgaaaa tcgcagcagc aaagcagctg tccgtctgcc tggcgtgttg tgttaccagg
    14941 tcgataacct ttcgcaagca gcgttggtca gtcatattca gcacatcaat aaactcaaga
    15001 ccacgttcga gcatatcgtc acggttgaat cagaactccc caccgcggca cgttttgaat
    15061 gggtgcatcg tcatttgccg gggctgatca cccttaatgc ttaccgcacg ctcaccgttc
    15121 tgcacgatcc cgccacttta cgctttggtt gggctaataa acatatcatt aagaatttgc
    15181 atcgtgatga agtcctggca cagctggaaa aaagcctgaa atcaccacgc agtgtcgcac
    15241 cgtggacgcg cgaggagtgg caaagaaaac tggagcgaga gtatcaggat atcgctgccc
    15301 tgccacagaa tgcgaagtta aaaatcaaac gtccggtgaa ggtgcagccg attgcccgcg
    15361 tctggtacaa aggagatcaa aaacaagtcc aacacgcctg ccctacaccg ctgattgcac
    15421 tgattaatcg ggataatggt gcgggcgtgc cggacgttgg tgagttgtta aattacgatg
    15481 ccgacaatgt gcagcaccgt tataaacctc aggcgcagcc gcttcgtttg atcattccac
    15541 ggctgcacct gtatgttgca gattaacgcc cggctttcat actgccgacc atctgttctg
    15601 gccgtaccca gctgtcaaac tcggcttcgc taagataccc cagcgcaagg gccgcagctt
    15661 ttaaggtcag cccttcttta tgcgcttttt tggcgatctc cgcggctttg tcataaccaa
    15721 tgtgggtgtt aagcgcagtc accagcatca gcgattcatt gagtaattga ttgattcgct
    15781 cacgattcgg ttcaataccc actgcgcagt gtttgttaaa actttccatg ccatctgcca
    15841 gcaagcgcac cgattgcagg aaattgtgga tcaccattgg acggaagacg ttcagttcaa
    15901 agttaccgga agcgcccccc atgttgatcg ccacgtcgtt ccccatcacc tgacagcaga
    15961 gcatggttaa tgcctcacac tgtgttgggt tcactttccc cggcatgatt gagctgcccg
    16021 gctcattttc cgggattgag atttcaccaa ttccgcagcg cgggccagag gccagccagc
    16081 ggacatcatt ggcgattttc atcagtgacg cagccaaccc tttcaacgcg ccgtgcgcct
    16141 gaaccagggc atcacaggtc gccagcgctt caaatttgtt cggcgcggta acaaacggtg
    16201 cacaggtaat gactgccagt tcatctgcta cgcgacgcgc atactccgga tgggtattta
    16261 gtccagtacc caccgctgta ccgccaagag ccagttccgc tacgtgaggc aggctgtatt
    16321 cgatatgttt gagattatgc tcgagcatcg ctacccagcc ggaaatctcc tgccccagtg
    16381 ttagcggggt ggcgtcctgc aagtgggttc gaccgatttt tacgatatcg gcaaatgcac
    16441 gcgatttttc actcagcgtc tgtgtcaggg ttttaagctg cggaatgagt tgcttgcgca
    16501 gcgccagcag cgccgcaacg tgcatcgccg tcggaaagac atcgttggaa ctttggcttt
    16561 tgttcacgtc gtcgttaggg tgaactttac gttccatccc gcgcacgccg ccgagtaatt
    16621 cactggcccg gttagccagc acttcattca tgttcatgtt actttgcgtg ccggagccgg
    16681 tctgccagat agccagcggg aattcgtcgt catgctgtcc tgccagtact tcatccgccg
    16741 cctgccgaat ggcgctcgct ttctcttcag acaacaagcc taaatcttca ttaacttttg
    16801 ccgctgcgcg cttggttagc gccagcgcat gaatcagtga ggtgggcatt ttctccgtcg
    16861 aaatgcggaa atgctccagc gagcgttgag tttgtgcgcc ccacagctta tctgccggga
    16921 catcaatcgc ccccatcgaa tctttttcgc tgcgtactgt attcatggcc tactcctcac
    16981 ctgattaatt ttttctttct gttttgcttt cgttaagtaa cttttagctc actcattatt
    17041 tactatttga taacaaatgt ttggtctttc gtgccatgta aaaaaaccgc cccgaagggc
    17101 ggctctgttt atttcacaca gcgggtgcat tgtgtgagtt gtatctgctg gaagaagtca
    17161 tttcctttat catccacaag gataaacgcc gggaaatctt ccacttcaat tttccagatg
    17221 gcttccattc ccagttccgg atattcaaca cattccaggc tcttaatact tccctgcgcc
    17281 aatacagcgg ccggaccacc gatactgcca aggtagaagc caccgtgttt tttacaggca
    17341 tccgtcacct gctggctgcg gttgcctttc gccagcatga tcatacttcc gcccagcgct
    17401 tgcagttgat cgacataaga atccatccgt ccggcggtcg ttgggccaag agaaccggag
    17461 gcataacctt ccggcgtttt ggccggaccc gcgtagtaaa tcggatgatc tttgatgtac
    17521 tgcggcagcc cttcaccgtt atccatccgc tctttcagtt tggcgtgagc aatatcacga
    17581 ccgacgataa tcgtgccgtt aagcgataag cgtgtagaaa cgggatactg cgacaactgt
    17641 gcgaggatct ctttcatcgg acggttaagg tcaacgcgca ccgcttcgcc ttctcccgct
    17701 ttgcgcagct cttccgggat atatttgcct ggattatgtt ccagtttttc gatccagatc
    17761 ccctgacggt tgatcttcgc tttgatatta cggtcagcag aacaggagac gcccataccg
    17821 accgggcagg atgcgccgtg acgtggcagg cgaatcacgc ggatgtcgtg agcgaagtat
    17881 ttaccgccaa actgcgcacc cagaccaaga ttttgcgctt cgatcagcaa ttctttttcc
    17941 aattccacat cgcggaacgc ctgaccgtgc tcattccctt ccgttggcag ttcatcatag
    18001 tatttcgcgg aagccagttt caccgtttta aggttcgttt ctgcagaagt tccaccaata
    18061 acgaacgcaa tatgatacgg aggacaggcc gccgtaccca gcgtgcgcat cttctcaacc
    18121 aggtaatttt tcagtttccc cggcgtcagt aacgctttgg tttcctgata gagatacgtc
    18181 ttgtttgccg aaccaccacc tttggcgata cagaggaatt tgtattcgtc gccatcaacg
    18241 gcataaagat cgatctgcgc tggcagattg gtgccggtat tcacttcttt atacatatcc
    18301 agcggcgcgt tttgcgagta gcgcagatta tcttcgatat aagtgttata gacaccgcgc
    18361 gccagcgccg cttcatcacc accaccggtc catacacgct gccctttttt accaacaata
    18421 atcgcggtgc cggtatcctg acaggttggc agaacgcctt tcgccgcgat gtcggagtta
    18481 cgcaggaatt gcagcgccac atatttatca ttttcgctgg cctccgggtc acgcagaatg
    18541 tcggccacct gttgttggtg cgccggacgc agcatgaacg acgcatcatg aaacgcctgg
    18601 cgcgccaaca gagttaacgc ttcgggtgcg actttcaaaa tctcctgccc ttcaaattca
    18661 gataagctaa cgtgttcgct ggttagcagg taatactcag tatcatcttt tttgagtgga
    18721 aaaggagcct gataatgaaa gggtttgttt gacattgttt tctcacttac tgcctggttt
    18781 ggttatgctc tgggcgggtg ttccgttgcc ctgttaaaag cgagtaacaa tatcctacgc
    18841 acttttttaa caaaaactga gactagtacg actttttgcg gctccaggtt acttcccgta
    18901 ggattcttgc tttaatagcg ggattaattt ccacattaaa acagggattg atcatgcaaa
    18961 aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt
    19021 atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga
    19081 aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg
    19141 agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt
    19201 tcctgttcaa agtattatgc gcagcacagc cactctccat tcaggttcat ccaaacaaac
    19261 acaagtctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg
    19321 agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc
    19381 ttgcgatgaa cgcgtttcgt gaattttccg agattgtctc cctactccag ccggtcgcag
    19441 gtgcacatcc ggcgattgct cactttttac aacagcctga tgccgaacgt ttaagcgaac
    19501 tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa
    19561 aatcggcgct cgacagtcag cagggtgaac cgtggcaaac gattcgttta atttctgaat
    19621 tttacccgga tgacagcggc ctgttctctc cgctgttgct gaatgtggtg aaattaaatc
    19681 ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc
    19741 tggaagtgat ggcaaactcc gataacgtgc tgcgtgcggg tctgacgcct aaatacattg
    19801 atattccgga actggttgcc aatgtgaagt tcgaagccaa accggctaac cagttgttga
    19861 cccagccggt gaaacaaggt gcagaactgg acttcccgat tcctgttgat gattttgcct
    19921 tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt
    19981 tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac
    20041 cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc
    20101 gtttagcacg ggtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct
    20161 aagcttatta aaggcttata acaccttcag gcggccagtc cgcctgattt cattttatgg
    20221 ataatcatta tgaataaatc gctggtagcg gtaggcgtca ttgttgcgct aggcgtagtc
    20281 tggacaggcg gcgcatggta tacaggcaag aagattgaaa cccatctcga agacatggtc
    20341 gcgcaggcga acgcgcaact caaactgacc gctcctgaat ccaacctgga agtgagttat
    20401 caaaactatc atcgcggcgt attcagcagt cagctgcaac tgttggtgaa acccattgcc
    20461 ggaaaagaaa atccgtggat taaaagcggt cagagcgtca tcttcaacga atcggttgat
    20521 catggtccct tcccccttgc ccagcttaaa aaactgaacc tgatcccgtc gatggcatca
    20581 attcaaacca cgctggttaa taacgaagta agcaaaccac tgtttgatat ggcaaaaggt
    20641 gaaacgcctt ttgagattaa ctcgcgcatt ggttacagcg gtgattccag ttccgatatt
    20701 tcgctcaagc cactaaatta cgagcaaaag gatgaaaaag tcgcctttag cggcggcgag
    20761 ttccagttaa atgcggacag agacggcaaa gctatctccc tttccgggga ggcgcaaagt
    20821 ggtcggatag acgcggttaa cgaatacaac cagaaagtac agttgacctt taataatctg
    20881 aaaaccgacg gttccagcac gctggcaagt tttggtgagc gcgtaggaaa ccaaaaactg
    20941 tcactggaaa aaatgaccat ttcagtggaa ggcaaagaac tggcactgct ggaaggcatg
    21001 gagatcagcg gtaaatcgga tctggtcaat gacggtaaaa cgatcaatag ccaactggat
    21061 tactcgctaa acagcctgaa ggtacagaat caggatctgg gcagcggcaa gctgacttta
    21121 aaagtcggcc aaattgatgg cgaagcctgg catcagttta gccagcaata taacgcgcaa
    21181 actcaggcgc tgctggcaca gccagaaatt gccaacaatc ccgaacttta tcaggagaaa
    21241 gtgacggaag ccttctttag cgccctgccg ctgatgttga aaggcgatcc ggtgattact
    21301 atcgcgccgc taagctggaa aaacagtcag ggtgaaagtg cgctgaatct gtcgctgttc
    21361 ctgaaagatc cggcaacgac taaagaagcg ccgcaaacgc tggcgcagga agtagatcgt
    21421 tcggttaaat ctctggatgc gaaactgacc attccggtgg atatggcaac tgagttgatg
    21481 actcaggtag cgaagctgga aggttatcag gaagatcaag cgaaaaaact ggcgaaacag
    21541 caagttgaag gtgcatcagc aatggggcag atgttccgtc tgaccacctt gcaggacaat
    21601 accatcacca ccagcctgca atatactaac ggtcagataa cgttaaacgg gcagaaaatg
    21661 ccactggaag atttcgttgg tatgtttgca atgccggcat taaatgttcc ggtcgtaccc
    21721 gctattccgc agcagtaatt cacagccaaa acgccggaga tttttctctc cggcgttatt
    21781 ttttacttca gcataaagtc atactgaata ctcattttca catcccagta attcaggcga
    21841 tcgcccagcg tactgccatc gcgcttttcc cgccctttat cggcagcaag aaagaaattc
    21901 atcccttgta acggcccttc gctgacccga tagttcatac cacctaagat acccgcgcct
    21961 tcaatccgca catctcgttt actggattca tagccgcgag tcacaatcgg tgcaaatgtc
    22021 agtgtaaatt gggagttaaa cgatagttga cgcccaattg ccaggactgc attccttcac
    22081 ggttacgatc aagacttaat gaaaagggat agccaatgtc ggtatccacc acggcaccat
    22141 tcattgcatg gcgcaaataa ccaccactgc caaatacgct tccccacggc gcattccagg
    22201 tcaattgtcc actaaccaac gccgtttcat tgggctggct ggtattacga cttaaacctt
    22261 cgagttgcgc gtaaaagccg agcaactccc ctttcaacgt ataattcggg tcgagcggta
    22321 tgcttttctc gagaatactt tgtatctgat tggaaacatc tttctgtacc gatgccccca
    22381 gagaatagcg actggtatca tcatgccaac tggcggctaa aaaagaacga gcgcgatgtt
    22441 tatcgtattt tcccgtctgg ctgttataca ccccttcatc acgaataccg gttgttgaat
    22501 cagtccataa aaatactcgt gaacgccaca ttccccacag atcaatattt ggcgttaaag
    22561 cccaataacc taatgctcct tcagaaactg tcggtaatgt acgttgcgaa ctgccagcca
    22621 ttaacggaat acgataaggt aagctaccat aactataatc cgtcccgaag cgacctaatt
    22681 taagcaggct attttcactg ggttttattt ttactgcccc gagggcaaag ccaaaacttt
    22741 tatcaccatc aagataccac cgggtactca tatcagcacg agcacctaat ttataaacat
    22801 aatacgctcc gccttcaacg ccaataatat tggaataata accactattg aaatcgagca
    22861 atcctccctg tacccatgcg taaatatctc ttcccgcgcc agcactgggc ttatcagccc
    22921 tgcgcaattc gtttttcagt ctgatgcgta gtcccgcttc atcgtcagcc agttgcgccg
    22981 cgtaagcaga ggtaaggcca gaggcagccg tcaggcaaat aacggccatg gccactattt
    23041 ttctc
//
LOCUS       DAZFDC010000072        22792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFDC010000072 DAZFDC010000000
VERSION     DAZFDC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.072"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            740..4552
                     /locus_tag="ACHJ48_002886"
     CDS             740..4552
                     /locus_tag="ACHJ48_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFO4708402.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINARGSIENRDGNNFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRLIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNSL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4641..6260
                     /locus_tag="ACHJ48_002887"
     CDS             4641..6260
                     /locus_tag="ACHJ48_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO4708403.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAIAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTSVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGISEYLSAKATHTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIENGYYNTAFFAQYAPRELTLQDKITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGSAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            6276..6644
                     /gene="acpS"
                     /locus_tag="ACHJ48_002888"
     CDS             6276..6644
                     /gene="acpS"
                     /locus_tag="ACHJ48_002888"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO4708404.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTSHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6658..7419
                     /locus_tag="ACHJ48_002889"
     CDS             6658..7419
                     /locus_tag="ACHJ48_002889"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO4708405.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIERQAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            7423..7971
                     /locus_tag="ACHJ48_002890"
     CDS             7423..7971
                     /locus_tag="ACHJ48_002890"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFO4708406.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            7993..8274
                     /locus_tag="ACHJ48_002891"
     CDS             7993..8274
                     /locus_tag="ACHJ48_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO4708407.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            8310..9470
                     /locus_tag="ACHJ48_002892"
     CDS             8310..9470
                     /locus_tag="ACHJ48_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFO4708408.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISAAELIASMNTILEKAEELDIQSMPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTEEDLLLFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQAVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKALFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            9544..12102
                     /locus_tag="ACHJ48_002893"
     CDS             9544..12102
                     /locus_tag="ACHJ48_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFO4708409.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVKDSERFA
                     KWFENARAAEVLHDIEYSDLNEDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSHARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSEATLPETESNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            12107..13330
                     /locus_tag="ACHJ48_002894"
     CDS             12107..13330
                     /locus_tag="ACHJ48_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFO4708410.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMTNMQAGRAK"
     gene            13327..14280
                     /locus_tag="ACHJ48_002895"
     CDS             13327..14280
                     /locus_tag="ACHJ48_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4708411.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            14291..14998
                     /locus_tag="ACHJ48_002896"
     CDS             14291..14998
                     /locus_tag="ACHJ48_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4708412.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNVFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            14982..15638
                     /locus_tag="ACHJ48_002897"
     CDS             14982..15638
                     /locus_tag="ACHJ48_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708413.1"
                     /translation="MTQMHKGFLLIGVFLYAAFVAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            15639..16949
                     /locus_tag="ACHJ48_002898"
     CDS             15639..16949
                     /locus_tag="ACHJ48_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4708414.1"
                     /translation="MSGEILAVNCVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLAADISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            16958..17746
                     /locus_tag="ACHJ48_002899"
     CDS             16958..17746
                     /locus_tag="ACHJ48_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFO4708415.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVTCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            17743..19131
                     /locus_tag="ACHJ48_002900"
     CDS             17743..19131
                     /locus_tag="ACHJ48_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708416.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSFSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDNFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            19145..20086
                     /locus_tag="ACHJ48_002901"
     CDS             19145..20086
                     /locus_tag="ACHJ48_002901"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO4708417.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMFNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            20083..21069
                     /gene="ycdU"
                     /locus_tag="ACHJ48_002902"
     CDS             20083..21069
                     /gene="ycdU"
                     /locus_tag="ACHJ48_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO4708418.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            21436..22638
                     /locus_tag="ACHJ48_002903"
     CDS             21436..22638
                     /locus_tag="ACHJ48_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4708419.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT         5962 a         5025 c         5946 g         5859 t
ORIGIN      
        1 aataacattt ttatgaagac cagaatacgt taagggatga tgtatagcgg tgattgctat
       61 tagatgggaa tgaaagcttg ccaaaccgtt ttgctcttct ttctcccttt caacgataag
      121 ccaggcataa tgtggctgct taagccaatt gtcgcaaagc caaagctctc tatatagcta
      181 acctcatggt taatccgatc tcagtaatac catgagcaag aactctttcc tttttatata
      241 actcttaagg gttacatgcg tgaatgcaaa tggtaatata atgaaatgtc gcctgcccgt
      301 aatgaccata aagaatctac cccgacgtaa aagatataga gaataagatg cagtaatgat
      361 gatgctttgt taatcaaata ataagattta aattaattgt aactcaccaa ctttgttgtt
      421 ttattcggat gtctgaaatg atgtcgtaca gaaaactaca tatcatttca gacgtcctct
      481 aaattggatt tttttcaccc aaggagcaag gatgggggac ttttaaatta ttgtgtttta
      541 gtaagttagg ttcttcgccg ttcgatgaaa aaacaataac tcttagttgt ttttttgagt
      601 ccttatttca gcttattttt cgtgattttt tattgcgcca ggtgatagga tttgccgcaa
      661 tcatttttgc tgatattccg tgagtcatca aaagtgatct gcatgtccaa aatgaatagc
      721 atcaataagg aaaacgttag tggcgatgat aaatttaagt aaggaagcaa cggtggggaa
      781 agcattaacc cctattgcta tacttatgat gttgtctttt cctgtagctt ctcaagcggc
      841 gggattagtc ataaaaaatg gaacggtata taacgccaat ggtgtgccag tcgttgacat
      901 caacaaacct aacggtagcg gtttatctca taatatctgg gataacctaa acgttgataa
      961 aaatggtgtc gttttcaata atagcgctaa tgaatccagt acttcacttg ccggaaatat
     1021 tcagggaaac agtaatctga cctccgggtc ggcgaaggtg atcctgaatg aggttacttc
     1081 caaaaatcct tcaaccatta atgggatgat ggaagttgca ggggataaag cggatctgat
     1141 tattgccaac ccgaatggta ttactgtaaa cggtggcggt tcaatcaata caggtaaact
     1201 taccttaacc accgggacgc cggatatcca ggatgacaag ctggccggtt actccgtgaa
     1261 cggcggtacc attacgctcg gtaaactgga taacgccagc ccgacagaaa ttctgtcccg
     1321 taacgtggta gttaacggca aagtgtctgc cgatgagctg aacgttgttg ctggcaataa
     1381 ctatgttaat gccgcaggcc aggtgaccgg tagcgtatcc gccacggggt cccgtaacgg
     1441 ttacagcgta gatgttgcca aactgggcgg aatgtatgcg aacaaaatca gtctggtcag
     1501 caccgagaaa ggtgtggggg ttcgcaacct cggcgttatt gctgggggtg ttaatggtgt
     1561 cagcatcgat tccaaaggta acctgttaaa cagtaacgcc cagattcagt ctgcaagcac
     1621 gatcaacctg acaacaaatg gtactctgga taacaccacc ggtacggtga catctgtagg
     1681 cactatctcg cttaatacca acaagaatac tatcgtgaat acccgtgcgg gtaacatctc
     1741 tacgatgggc gatatctacg ttaacagcgg tacgattgac aatactaacg gcaagcttgc
     1801 ggctgcagga atgctggcgg ttgataccaa taacgccacg ctgattaact ctggtaaagg
     1861 gagttctgtc gggattgaag cggggctcgt ggcgctgaaa accggaacgc tcaacaacag
     1921 caatggtcag attcgcggtg gctatgtggg tcttgaatcc gctgcgctga ataacaacaa
     1981 cggtgatatc cagaccaccg gcgatatcgc cattatcagt aacggtaatg tggataacaa
     2041 caaaggtctg atccgttcgt ccaccgggca tatcgttatt ggcgcggcag gtagcgtaaa
     2101 taatggttca accaaaaccg ccgataccgg cagttctgac tctctgggca ttattgcaga
     2161 taccggcgta gaaattggtg cgaacaacat caataacaac ggcggacaga ttgcgtctaa
     2221 tggcaacgtc tccctgtcaa gttacagcac gatcgacgac tatgcgggca aaattctgtc
     2281 caacagcaaa gtgattatca agggaagctc tctgcgtaac gataccgggg ggatcagcgg
     2341 taagcagggt attgaagtcg ccgttggcgg cagcctgacc aataatattg gcgtgatcag
     2401 ctctgaagag ggtgatatct ccctgttagc caactccgtg gataaccacg gcggcttcat
     2461 gatggggcag aacatcacga tggagtcgat gtctggcgtc aataacaaca cagcgctgat
     2521 cgtggccagc aaaaaactga agataaatgc gcgcggcagt atcgaaaacc gcgatggcaa
     2581 taacttcggt aatgcttatg gtctgtactt cggcatgcct cagcaaacgg gtggaatggt
     2641 cggcaaggaa ggcatcgagc tttccgggca gaacatctat aacaacaaca gccgtcttat
     2701 cgctgaggat ggtcctctga ctctgcaggc gcagaacacg ttcgacaaca cgcgtgctct
     2761 ggtcaccagc ggggcggatg catctattca ggttggcgga acgtattaca acaactacgc
     2821 taccacctgg agtgcgggca acctggatat cgacgcgacc acgctgcaaa acagcagcag
     2881 cggtacgatg atcgataaca atgcgaccgg gttcatagca tctgataaaa acctgtcact
     2941 ggaagtggtg aatagcctta ccaactacgg ctggatcagc ggtaaaggcg atgttgatgt
     3001 cacggtgaat aacggcaacc tgtataaccg caataccatt gcggctgaaa aggggctgga
     3061 tattgccgcg ttgaacggta ttgaaaactg gaaggatatt tctgctggcg gcgacctgac
     3121 gatgaacacc aatcgccatg tgaccaacaa ctccaacagc aatatggtgg ggcagaatat
     3181 tgttattaac gcggttaacg atatcaacaa ccgtggcaac attgtcagtg acgctgacct
     3241 gaacgtgacg accaaaggca acctgtataa ctatctctat atggtagggt atggggatat
     3301 cgcattgtcg gcaaatagcg tggcgaacaa taacgcgacc atcgaagcga caggcgatct
     3361 gattatcgat tcgaagggta acgtgggtaa caaccgcggt aatctgcatg cgttgaacgg
     3421 cgtgttgtct gttaaaggca acaatctgaa caacgataac ggtgaaattc gtggttatgg
     3481 cgatgtcacg ctggcactga cgggcaacta cgacagctat aagggttcgc tgacctctga
     3541 aacgggcgac gtgactctga cggcgaacat tgtagacaac gcctatggtt tgattgccgg
     3601 tgagaatgtt tctgtcgatg ctaaatcgac gatttacaac aacactgcgc tgatcgcggc
     3661 gaataaaaag ctggttatta acgctggcgg caacctcgaa aaccgcgacg ggaataactt
     3721 cctgcgtaat aacggcgcgc tgtttggaat taccgacaac gttggcggca tcgtaggtaa
     3781 agaaggtgtc acgctttctg ctcagaacgt ctacaacaat aacagcagca tcatcgctga
     3841 aaatggtccg cttaatctgc tgtccagggg aacgctggat aatacccgcg cgcttcttag
     3901 cagtggggct gatgccatca tccgtgcggc agggacgttc tacaacaact atgccaccac
     3961 gtacagcgcc ggtaatctcg acgtttatgc ggcgtcgttg aacaacgcca gcgatggtcg
     4021 cctggaagac aataccgcca cgggcgtgat tgcgtctgac aaaaacctgg atctgagcgt
     4081 tgataacagt gtcactaact atggttggat cagcggtaaa ggagatgtgc atttcaatgt
     4141 tctgaaaggc acgctgtata accgtaatgc catcgcggcg gacaacgcgc tgaccattaa
     4201 tgccctgaac ggtgttgaga actttaaaga cattgtggcg ggtactgcgc tgactattga
     4261 tacgcagaag tatgttacca acaacagcaa cagtaatatg ttgggacaaa ccatcgcgat
     4321 caatgccgtg aatgacatta ataaccgtgg aaatattgtg ggtgattatt ctctgggtgt
     4381 taaaaccacc ggtaatattt ataactacct caatatgctg agttatggtg tcgctggcgt
     4441 atcggcaaat aaggttacga atagcggtaa agacgctgtt ctcggtggct tctacggttt
     4501 agcgttagaa gcaaacgaaa ctgataacac cggtactatt gtcggcatgt aagatgtaac
     4561 caaagacagc agtggctttt aatattaatg cgtcatgtca cgcaggggtt gctgctgtct
     4621 ttatttagtt tgagaagata atgcaattac gatttaattt tggtttgctg ctcattcttt
     4681 gttgttttca ggttgcagcg aaagacagtc ttgataattt gatcaagcag caaaaaaatg
     4741 cagatgaaag cacgttgcgc gcgcataaat tagaaaaaaa agatgtttac tcaaatgcac
     4801 aacgtaatgc gtttactctg ggtgatctgc cgcaggaaaa gaattgcttt atcattaatc
     4861 ggattgaact ggaagataac tttctgggtg gtcagttgat ttctgaagtg caaaaagcga
     4921 ttgccggacg ttgtgtcggt agtgctggtg tgcaaaaaat tgcagctgcc ttgcaggatt
     4981 attatattaa tgcgggatat atcacaacgc gaattagtat cccttcgcag gatatttcta
     5041 aaggcaagct acgtttccag gttaatgccg ggaagataga aaaaacgatt gtcgaaggta
     5101 atgatatcag tgaatggatg ttaccttttc agcgtaacga tattctcaat attcgtgata
     5161 ttgaacaggg gctggaaaat cttcagcgag tacctgacgt tgatgtgaaa attaatattg
     5221 aaccgggaac acgggatggt tacagcatcg ttcatattga tacacaccgg gggaaaaact
     5281 ggagcgttcg ggcaagttac aacaattggg gcgatgaaga aacgggccgt taccagacaa
     5341 cctcggtggg ttatctgttt aatccagcga aaatggggga tctgttttat ctcgccggta
     5401 cgcgtagtac taccggacag tatgaaaacg tcagtagtta ttattccatt ccggtggggt
     5461 actgggagta cggctttttc tatagcaaaa gtaaatcgca gcaggtgatt ccgctttcgt
     5521 acatctcgct ggattatgtt ggcatcagcg aatacttgag cgcaaaagcg acgcataccg
     5581 tttatcgcga taaaacaaaa aagttcgccg gtagtgcaga actcattcga cgaaagtcga
     5641 attacacgct taatggtgag gagctgacat tacaggcgcg ggatatggga aatatcaagc
     5701 tggggctaaa ttataagcaa cagttacccg gcgcgttctg ggattcaacc ctctcgtggc
     5761 aacgcttttt aacctggttc ggtggtgaaa aaacgccgga tatgcaatat ggcgacgtta
     5821 gcccggtaag caatatcttt aattttgagg gtaactatac ccgacaaata gagaatggat
     5881 attacaatac cgcttttttt gcccagtacg caccgcgtga actgactttg caagataaaa
     5941 ttacagttgg cgatcgttgg agtattcgcg ggtttgaaaa tagcgtaggt ttgtcaggga
     6001 atgatggttt ttatataaaa aatacgctgg catttccttt gcctggaatg aaagctaatt
     6061 attatgccgg tcttgatttt gggcaagtgt atcaggacgc ttcttatggc gatgaatcac
     6121 tggtgggctc ggcagttggt attgatggaa acattaaaag tcttgagtat aacttttccg
     6181 tttctacccc attaaagtat ccggcgacat tagatattga tcgggtgaat gtaaatttta
     6241 atttttcgta ccaaatgtaa aggataacga gactcatgag aatagggaca gatattgttg
     6301 aaattgccag aataaccaaa gccattatta atggagagat ggctttttat gagcgtgttt
     6361 atacttctca tgagcgctta ctcattaacc ctgcagaacc tgattatgaa cgggcctctg
     6421 gattctgggc ggcgaaagaa gcgttagtta aagcgattgg ttgtggtttt cgtgatggcg
     6481 tacgtttcca ggatattgaa ataaagcatg atgcagccaa agcaccttat ttcgttattt
     6541 ccggaagact acaagaaatt tttatcgggc aggggttgca aaataactcc ctgagtattt
     6601 cgcattgccg tacccacgcc attgcgactg tcatcgttta ttaactcaac atatgtgatg
     6661 aatatgtaca aatataatga tttaaatgaa aaaaccgtac tggtgacagg agccagtggt
     6721 gatattggac tgggtatttg cgaaaaatat ctggagcaga actgcgatgt ttatgcgctc
     6781 tataaatcca acgacgtgca attaacggcg ctgaaagcaa gccatcctgc aggcgataaa
     6841 ctgcatattg tgcagtgcga tcttgcctgc ccccagagcg tgagcgcatt gtgcgaacag
     6901 atagagcgcc aagctggcaa gattgatgtg ctggtaaata acgcgggcat cgttaaagac
     6961 agcctgtttg cctcgatgag ttatgaggat ttcacccagg ttatcgaaac caacatgttc
     7021 agcattttcc gtctgacgaa agacgcgctg atgctgcttc gtgcggcaga aaatcccgcc
     7081 attattaacg ttgcctctat tgcagcgctg atcccaagcg tcggtcaggc gaattacagc
     7141 gcgtcaaaag gggcgatcct aggcttcacc cgcacgctgg cagcagaaat ggcaccctgg
     7201 ggcgtgcgcg tcaacgcggt cgcaccggga atgattgaat cgaaaatggt gaaaaaagta
     7261 tcccgcgcag tggtccgtgc ggtaaccagt accatcccgt tacgccgtct gggtaaatgt
     7321 gaagaagtgg cgaacaccat cgtcttctta agttcctctg cctccagcta cattgtcggc
     7381 cagaccatcg ttatcgatgg cggcctggtc atgcggtgat ttatgtctaa atacacgatt
     7441 ccttcgaaaa tcttcctcga aatgggcggc tggcgtcagc cgctgctgat ggtcgataaa
     7501 attgcagatt ataaatacgg tgaaaatggc tttgtcagtg tcgtgaaaca cgtcacctat
     7561 aacgaaccgt atctactggg gcatttcccg gaagatccga ttatgcccgg ggtgattatt
     7621 tcggaaatct tcggccaggc cagcgagtat ctctctttcc tgaccgacat ttgcgatatc
     7681 tggcgcgaac gttttgagga agagctgaag tcgttacgtg atattcacgc acgcattcac
     7741 cgccctgaaa tgctggagat tatccgtacc cggcgcagcc aggtacgcgg cgtgctggcg
     7801 gctcagaacc tgaaatttaa agatatcgcc tatccgggtg attctattga tgttgtcagc
     7861 aaattggctt tttcggatgc cagtggcttt aaacactatt ccgtgaccgc gtacgtcggt
     7921 aaaaagctga ttagtcaggg caccattatc aatttccgtg aaaccaagta atccaataag
     7981 agagtaaata ttatgtctac catgaatgac gctattgaac agcgtaaaga agttctgatg
     8041 accattaaaa ctgagatcat ccagcgactg aatattcaac gcgatgaagc gcaaattgat
     8101 gatgatactg cgctgtttgg taatggtttt aaactggact ccgtcgatgc aacggaaatt
     8161 gtcgttctgc tggataaagt atttgatatc aagatcagcg aaagtgacga cccgtcctat
     8221 atgcgcagta tcaatagtct ggccaccttc gttattcaaa agaaaaacga ttaactactt
     8281 attaaagaat taattatcag gacacgttta tgaaatctct tgctgaattt cacttgaaaa
     8341 ataatgcggt aatgggggta tataacaacc gtaccctacc ttcttcttat cacgatgcta
     8401 tgaccgaata taaagccgtt cgtgaaaacg cgctgctggt ggactattct cacctctcta
     8461 ttgttagcgt catgggcgat gatgcctggg cgctgatcaa ccagctggtt tccgcagatg
     8521 tctccatcat tcgtgatgag caggcaatct actcactggt gttgaatgaa gagggcacca
     8581 ttcgcggaga cgtctatgtg ctgtgcagca ttgacggtta ttacctgctt tcagaagata
     8641 tttcggctgc cgaattgatc gccagcatga ataccattct cgaaaaagcg gaagaactgg
     8701 atattcaatc gatgcctgaa attcaggata tgcgggaaaa caactggggg gcaatcctgc
     8761 tcgaaggccc atactcctgg gagatcatgt ctgaaattca tggctttgac gtgattggcc
     8821 tgccttacta cgaatacatg aataccgagg aggatctgct gctcttccgc tgtggtaaac
     8881 acggtgagta tgcgtacatg accatcggtg agcaggcgaa actggcggag cagtgggaaa
     8941 aactgttaac cgttggcgag aaatacctga tgcagaccgg cggcctggat tatcaaaaaa
     9001 tcgtacgcct cgaaaatccg tgctgggatg ccagcctctg ggaaggtcag gcggtgaatc
     9061 cggttcagtt gcagatgcag tgggcggtgc agtacgacaa agatgatttt attggcaagg
     9121 acgcggtgac agagctatcc caggagtata ccggtaataa actcatcggc atgatcgcac
     9181 aggaagaatg cgaaggcatt gaggctggcg atcgcgtgct ggtggaaggt caagacgtgg
     9241 gctatgtcgt gaaggcactc ttttccccag cgttgcagcg tttcattgcc ctgaccctgc
     9301 tggaaaagga ttacgcctgg tctgacatca gcggctacga aattcaaact gctcacggaa
     9361 ttattccggc gcaatcaaaa tgtatgccgt ttatttataa cctgagcatg ttggttagcc
     9421 caactgagca cagttatatc gacgcgtcaa aaaataaaag cgcagcgtaa tgaatatcgc
     9481 cgcgtgacta tagcttacgc ggcttaaatt aaccccaatt ggcgggattg ccaggtatta
     9541 ttcatgaaaa cgaacaggaa aagagttgtt attaccgggc tggggatttt gtcctcactg
     9601 gcggaaaata ttcaggattt ccgggaagca ttattaaata aaaaaaatgg cgttaaggat
     9661 agcgaacgct ttgcaaaatg gtttgaaaat gcgcgtgcgg cagaagtgtt gcatgacatt
     9721 gaatactccg atctgaacga agatgttatc gattcactgg ataacgcagc gctctgggcc
     9781 tacaaagtgg gtaaagatgc gctgagtcag gccggcctga tggatagccg tgacaccctc
     9841 aaagaaacag gcatgatcgt cggtgtttcg tccgcgggaa cagaggcatt tttgccgctg
     9901 ttcgaacagc gcatggaaga tttctcgctg cgtaaagcac tcttttccgg cgcattctca
     9961 tcctgttgtt ccagcgtttc aacgctgctg ggcttacagg gtggggttga actggtggcg
    10021 accgcgtgta ccgccagccc gaacgccgtg gggatggcct ttgactacat ccagaatggt
    10081 aaaagcaaaa ccatgctggc agtgggcact gagcccatct atctgccgac ctttgccgga
    10141 ttctacgcac tgaatgtcat gcatccagac acttgtacgc cgttctcggg tcagtccggt
    10201 atgtcgattg gtgaaggcgc tggcgctatc gtgctggaag agtatgagca cgccgtagca
    10261 cgcggggcga ccatttatgg tgagatcctc tcttacgcca cttcctgcga tgctttccat
    10321 gaaaccgggc cagatccgcg tgccagcgga gcggtgcagg tcatgaacaa ggcaatggca
    10381 aatgcgggcg taaccccgga gcaaatcgat tacgtcaacg cgcacggtac gggtaccgaa
    10441 gccaatgacc gtattgaaac gctggcaatg aaaaaggtct tcgctaacca cgataaactg
    10501 ctggtgagct ccaccaaatc ttactttggc cacaacattg gcgcagcggg gattgtggaa
    10561 ctgattgcct gtctggcaac gcttccggat aaccgcgtac tgccgacgct gaatttcagc
    10621 catgcacgcc cgaattgcga tctggactat gtgccgaatg agtttcgcga tcgcaaagtg
    10681 aacatcttca tgaagaataa ctatgccttc ggcggcaaca actgctgcat gatcctgagc
    10741 atggagcccg cgtccattcc ggtcagtacc tatgattcga agcgtgtggc tatctcgggc
    10801 gtcggtgcag tatccgcaat aggccacacg ctcaaccagg ttctggaaaa catctgggcg
    10861 caggaccacc acgttgaact gggtagcgta accttcccgg acgatacact ggaagaagcc
    10921 aaagagctgc tggccgtgct ggatgaatca aaccagtttg ccgagctgtt tgatgaagag
    10981 tacagcttca gcgaagctac gcttccggaa acggaaagca actttaaaac gttccaggtt
    11041 tccgggctgg agccgcgtaa acacctgcgt cgtttcgatc agcgtaaagc gacccgtggc
    11101 ggcacgtttg ccctgatcgc actcaccgaa gcgctggaac aggcgaagcg taaaatcaaa
    11161 cgcgacggcg atgagttggg catgatcatg gggatgtccc gcggcccgca ggaaaccacc
    11221 tacaaatatc tgcaaagtct gaaacccgat ccacgcaaag tacgtacatc ggaatttcct
    11281 ggctcgctga tgaatgcgat cccaacgttc tgcgggatct ctgaagggat caaagggtat
    11341 accactacgc ttgcgacagg ggaaaactcg gcgctgggcg cgctgaccta cggttatgaa
    11401 atcgtccggc agaacctgca accgcaggta ttggttggcg gcgcggatga gtacttcccg
    11461 tcgatgtcgc tgtatatgga tgcggtgaca caaaagatcc acatgacggc tgaggcgagc
    11521 gactatcaga tttacggtaa agatccgcaa ggttatgttc cgggcgaagg ggcttgcatg
    11581 ctgctgctgg aagatccgca gttggctgtg gcgcgtggcg ctgaggttct cgcggaagtg
    11641 gtgggctacg gcaagtcatg cagcaatacc tacttcgacg cctcattgtt ggaagaaaaa
    11701 tccgcgtcga tggcgctggc cattgaacgc gcccttacgc atgcgggcat ctctgctggc
    11761 gatattgatt tggtatgtgg taccagcaac ggtagcgatg accattctcg tatcgaaatt
    11821 gacgcgattt acaacacctt cgccgcgcaa aacccggcgg tgccggtagt gaactataac
    11881 gcttgctttg gttttgtcgc ctctgccgct ggtttgctta acctcgccgt gattactgac
    11941 tgcattaaga agcaggcagt acctgcgatt ccaaatacgg tggaattctt tgatgagcgt
    12001 gtgaattttg tacgccagtc gatgaaactt gcgctgaacc atgtcctgct tgttggcgca
    12061 acggaaggcg gcaactacta cgcgttcgtc attaagggat aagactgtgg acaatgccgc
    12121 tattatttcg ggttacagcg tgtggatgcc gtatgccgaa gactgttcgc agttaattga
    12181 caatctgaaa caggggaaac gcgttgcgcg tacaccctgg tttacctcca acgaggaagc
    12241 cattaaatgc gggtttaagg gtaatccgtc agtggcgacg ttgaaacagg tggacgatag
    12301 cgcattggac ctgctgtctc agttgatcga cgaagccctg gaacaggcaa tgctggataa
    12361 gcattgcctg gccgggcgca acgttcgcgt ttatctgacc ggtattgggc cgcgtatcga
    12421 cgggctggat tacaaatctt tctataatta caacgatgta gaagatatca acttaacgca
    12481 atccatcaca aatctgcatg cctcaaagat gtcgcaggac acgatttcaa gccatcttgc
    12541 gcgcaaatat cgcttgcagt atttgccgcc taacatgaac tgcaccagta actcgtcact
    12601 gactgcggtg catctggcga cgcagggcat tgagcagggc gggattgatc tcgcgattgt
    12661 gctgaactgt tcgaaaatta aaacccagga catctggttc ctggaaacgc aatcgatgct
    12721 ggacagcgaa caagtgcagc cgtttggtga aaacagtaaa ggtgtggcgt tcgcagaagg
    12781 ttttagcgcg ctcctactgg aaagcgctca tcaccgccgg gcgcgtcaac aaagtgaggg
    12841 cgtaagggtg caaacgactt acacccaaat cagcgcgggc cgcagcaacg atgcctcctg
    12901 gcttagcact aacgtgcaca aggtcatgca ggccgcgatg aagcaggctg aaattgcgct
    12961 ggatgatctt gcagccattc ttccacacgg caacggttcg gcagtgagcg ataacgcgga
    13021 ggccaaagcc atcgccatgt tcgcagggga gcgacagatc cccgttctcg cctataaagg
    13081 gcagatcggc tataccgcaa ccggatctgg tgttgtcgat ctgatcattg gccaccattc
    13141 gttgacgcat catcaactga tcgcacctgt tggcaacgac gtcattatcg acagcatggc
    13201 ttcgctggta cttacggacg gcagcgtgac aaaccatagc aaacgccatt tgctgaaggt
    13261 tggtgtgggt gtcgatggtt cagttattgg cgttgtcatg acaaatatgc aagcggggcg
    13321 cgcgaaatga cggaaaagag tatctacctg tcttcctggg cgcttacgga accggacgac
    13381 attttttatt tttatcagcc gacctggctc aacgcctggg aagcacatat gtacgaaagg
    13441 ggattatacg gcacccgtgc taatggctgg ggactgaagc gctttggcgt ggagaacgat
    13501 atttatgcac cagaacggat ccgtaatcca caatatatgc cttaccttga ggcgatggag
    13561 caggtgaccg attctgcatt acaacaaatg ccgactaaag aacgcatgaa gttaatgaaa
    13621 tcgcgcacgg catttattta tgccgattcc tggggcgaat ccggttcgtt tgaaaaaatt
    13681 tccagtgcgc tgcatatcgc cactattgat acgcttccca agaatttact taagaagttt
    13741 tcgataacgg aaccgacttg caagattcgc ggcgaaaaac aggcattcgt tcaggcgctg
    13801 cgtatggcgc aggattatct cgcctgggat gtattcgata acgtggtgat ttgtgctgcc
    13861 tatcgggcaa ttccggtgct ggtattctct gaagaagatg tgggtaaacc gcctaagcgt
    13921 ttatttcccc gtcgtgctga tgaggtgaat ttatctgttg aacgagtggg ttgttttatt
    13981 ctcagccagc gtgagagcgc catcaggctg caatgcgggg aatatcattc cggcactgcg
    14041 ccacagcatg ctacggatat tgatgtcatg gccttcagcg aacggcaaca aatgccgtcc
    14101 cggcctgctg ctggcgttat cgacctggtg gcccattacg gtgaaagcgg ttgtctgacg
    14161 cccgcgttga gtatggatta tttaacccgt catattcagc ccggtggaaa aatgcgtacg
    14221 gtcattgccg ataaacagtt tggttatcac tactttgacc ttgaatacct tgaaggataa
    14281 cgacagtcgt atgcaatcta ctattaaatc gttgcccgct atttcgctta gcaatattta
    14341 taagtgttat ggcgctgggg caggaaaggt tgatgcgctt aaaagcatta atcttgatat
    14401 caataaaggc gaatttctcg cactgtgtgg gccgtcaggc agtggtaaaa gtactctgtt
    14461 gaatattctc tcgggtattg ataaaccgac cagcggaacg gtgatatttt taaataaatt
    14521 actgaaccaa ctgccagaag agcagttggc tgtgattcgt ggaaaacatt tgggttttat
    14581 ttttcagttt tttaacctta tgccggtatt aaatgttttc gataacgttt atttcccact
    14641 ggtgcttaat ggtcagttca gtaaaaaaga agccactgaa cggacgctgc attatctcga
    14701 tagtgttgga ttaagcggtt ttggcgatcg taaacctggg caactatcgg gcggtcaaca
    14761 acagcgtgtc gccatcgcca gggcactggc ccacgaaccg atggtggtca ttgccgatga
    14821 accgacggga aatttagacc tggcaaccgg cgaggctatt ttggacctgt tgttgacgat
    14881 taatcagcag accggcacca catttgttat ttccacgcac tccagcgaat taaaagcgcg
    14941 ggcacggcga gtggttgaaa ttcaggatgg agtattgatt catgactcag atgcataaag
    15001 gatttttgct catcggcgtc tttttgtacg cggcgtttgt ggctccgaag atttacgcgg
    15061 caacacccga cgctgttaac caggttctgc aacaggggct cccggcgcag cctgccgcgc
    15121 tggcgacgct gaccgaggcg ttaaagaaag agtatgaagc caggcacaat gtggtggcgc
    15181 tggtttttta cgcctatggc ttactgcgtc aagcggacgg ttatgcggca acaaacgatt
    15241 ttatccatgc ctcagaatac gccaaaagcg gctttttctg gctggatgaa gcggtggatt
    15301 tgcaggagaa aaatcaacgc gtacggtatt tacgcgcccg cgtagatgcc tatttacccg
    15361 cagactcagg gcgttgcgtg gtgacagtca aagacaccga acacatgctg gctgacccgg
    15421 cgatatggac gacaacgatc cgcgaccata ttctggcgat gcgttatcgc gcattacgtc
    15481 actgcaagga caccacccgc gcgaatgcgt tgttggcgca aattaaaggg caaaacgccg
    15541 cgcttgcgca gtcgctgacg caggatttta atgttgtacc agaatgggat agtgaagagt
    15601 taacccaggt actgctgccg ctgatgaaag gtgagtaaat gtccggggaa attttagctg
    15661 ttaattgcgt tctgctgctg attctgtttt ttgtgtttta tcagcttctg ttttattcac
    15721 gagatgtcaa atttgcattt ttgaatctct ttcgccacaa gcgtcgctct ttttctacca
    15781 taaccgctat tgtattgggt ggcgtggcaa tttttttata tggcggattt attgattact
    15841 ctttctggat cctgaaagag cagacgattc gtaccaatat tggtcatgta caaatttaca
    15901 atcagcacta ttttgaaacg tcgaataaaa ataagagcct gattgccgat tatgcggcgt
    15961 taaagaaagc tattttgtcg gacccgacgc tggcggcgga tatttccacg ctctccgggc
    16021 aactggagtt tactggcgta atttcacatt acgagagcga aacctccagc tatttctccg
    16081 cgctgggggt cgagccgtta cccgctttaa agctgggttc gttcgataag attatcgcgg
    16141 ggagcgatct ttcccgggta aaaggtgatg aaatcaccct gggcagcggt ctggcgaaaa
    16201 cgttgaatgc gaaatatgac gactggctgg atgtgatggt ggtcaacacg gcgggcgggc
    16261 agggcgcatt gtcgctaaaa atgcgtggca tttttgagtc aggcattaaa gattatgacg
    16321 atgtcgcgat gaagatcccg ctggacaccg cgcagcgcat gatgggtacg gatggcgtca
    16381 gcaaagtgct tattctgctt aaagaggatg acaccgcggc gtttagcgca aagctgcgtc
    16441 aatttatcga acgcaatcat ttgccgctgg tggtcaaaga ctggcgtgat gtttcactct
    16501 tttatcaaca ggttgaaggg ttgctatcgg ggatctattt cttcatcaag ctgattgttg
    16561 cgctgattgt catcttcatg atcggcaact cgatggcaat gaacattgtt gagcgcaccc
    16621 gtgaaattac cactctgcgt gccataggcc tgaagccgct tcacgtcacg cgcttgtttc
    16681 tgacagaagg catttttatt ggtgtgattg gcgcagtcgg cagcctgctg gtggggggcg
    16741 tgttagcctg gattattaat ctttatggta ttgcgatgcc gccatcgccg ggacaaacca
    16801 tcggctatac cgcctttata aaaaccagta acccggaatt gatttgggtg acggtggtat
    16861 tacctatttt gacagcaaca ggtgcctctg tgttaccggc tttacgtgct tcccggctga
    16921 atatttctga cgcatttaaa ttctcatagg gctaattatg aatatggctt tttacggcaa
    16981 gtggtttgct tgcctgtggc tcgctacgtc gtgcgttcag gcggcgagca cagataataa
    17041 ggcgctggag ataattcgtc gcgctgacga aattcgttcg ccgaataaac cgtttcgtta
    17101 cacgctgacg gtaacggaat ataaagcggg cgcaacccag ccagagaata aacaggtcct
    17161 cgatatttcc atgcgcttta tgaaaccgca gggtaatgaa aaagcggatg cacgctcgct
    17221 ggtgcgtttt atttatccgc cgcgagataa aggaaaaatc atgctttccg actggtacga
    17281 tttatggttt tacacgccgg aactgcgacg tcctatgcct atttcacggc aacaaagatt
    17341 aattgggcaa atctctaatg gcgatgtcat tgtcactaac tttgaatatg cctatgactc
    17401 gaccctgatg ggggaagtca cgtgtgctga aaagcagtgt tacaagctgg cgctggtgcg
    17461 taaatcggcg gatatcacct ggccgaaggt tatctattac gttgaaaaag acggtgataa
    17521 tcgcccgtgg aaggcggcct attattcgca ggatgaccag ttaataaaag aagtgttgta
    17581 tcaggatttc cagccggtgt tggggaaaac gcgacccatg aaaataaccg ttaccgatgt
    17641 tcgtcacggc aataactatt cagtgatgga gtacagcgat gttcgtctgg aatcgctgcc
    17701 ggagtttcat ttcaccaaag agtatattca gcgaggtgcg aaatgaaacg tacgctgaag
    17761 atctcttcgt tgctgtgtgt tgcgctgccg ttgacggtac aggcggattg cctgtcaggt
    17821 gacgaggtgg cacaaaatag cgatgtgacg ctttacgagc aggtgtctta tgtcaataaa
    17881 caggcttcgg cctggcagat tgcaggcaaa aatccctaca cccgtcacaa cggttatcag
    17941 gaggcgggga ttggcatcaa tagcggttgt tcgataatcg acaatacgct ggaccttaag
    18001 ctcaatctgt atggaataaa cgaatatgcg cttaaacccg cagggaaatt cgaaacggat
    18061 gatagccgca cgcgtgcgtt aattaaccgc ctcagtctgg tctatagcgc ctcggacagc
    18121 gttcaatttg aagcaggcaa atttgccgcg ccgtcaggaa tgttcttttt gcgttcgcct
    18181 tctgatttgc aaacgcatta ttacaccgga ttccagtcga cgcgtttgca cgatcccaaa
    18241 atgacatcgg cgtatcaggc gtcatcgtgg ggcgcaaaaa tgagcgtgga tacacgcgat
    18301 tatgcgtttt cggcgagtgt cataccgaag ttagccacca tcgacaaacg ctatgtgacg
    18361 tctggcaact ggtcggctaa tcagcaggga aacagcgacg aagcctatct gctgagcttc
    18421 agcgatcatc gctttggtga gcacacgccg acagtcaatg tgcggctcgg accatcgccg
    18481 tcactcgcgc tgtctgacag ttatcactac accccgcagc ttacgctgaa tgtcgacgca
    18541 gcctaccacc gctcccagca atggcggcat ctttcccacc gtgagacggc gcaggtcgag
    18601 gaatatcagt ttccggattc gctctatgaa acgaaagatg aaagcggcgt agagctggcg
    18661 ctgggtgggc agtacaccag tgataatttc agcgtgtttg gcgttgagta ttactttcag
    18721 agcgagggtt attcgcgcgc tgaacagcgt cagcagcgcg agttaattga cttcctcaat
    18781 accacaaccg gctatgcgcc actggatcag gcatttgatt cttacaaata cctgatggct
    18841 tcagaaatca gcaataccgc caatcagggg atgctgcagg gtaagcacta tctgaacgcc
    18901 tgggccagtc tgccgctggc gggagagtcg acgctgcagc cttcgctggt ggtgaatctc
    18961 gttgatggca gcacgttgct gggcctgcat tattccacgc cgctcagcgc tataagtaac
    19021 cagcttgaag cctatgccgg tggctatagc gcgctgggaa gtcggtactc cgaatttgcg
    19081 ctttttggcg acacgttagg cctttatttg ggctttaaat actatctata aggataaaga
    19141 gatgatgaat aatgcttcac aaccgacagt actgcttttt gccggtcagg gtaatccggt
    19201 tattggtatg ggcagcgatc tttgggatct caacgccagt acaaaacata tctgggattg
    19261 tgccagcgat atttccggac tgaacctgcg tcgcctgtgt cagaaagggc cgatgaatcg
    19321 ccttattcaa acgacggtac aacaactggc ggtgaccgca attaatgtaa cgctctatac
    19381 cctgtgccgc gagcgtcttg agaatttaac ggttgtgggc tcctgcggac acagcgttgg
    19441 tgaatacagt gcgctctacg ccgctggagc tatttctctt gagacgctat ttggcatgat
    19501 tcacttcaga gccagcctga tgcatgacga aagccagaga aataaaggcg caatgctggc
    19561 ggttaaaggc atcaaccgcg aacaactgca gacgatgatt acggaatcag gcattgcgct
    19621 ggatattagc tgtgataaca cgccgcgcca gcaggttatt gggggaacgc aggctgcgtt
    19681 gaatgaattt gccacgctgc tgatggccgc tggatacgaa ccggttaaac tcggtgtgag
    19741 cggtgcctgg cacacgcgtt tgatggaaga cggcgttcag gcaatgcgcg attatcttgc
    19801 cggactggat atcgcttcgc cagagcatca ggttctgatg aacgtaacag caaaaagcga
    19861 ggtcgctccg tcaattatca aagaaaatct ctcattacac ttaactcata cggtgaaatg
    19921 gactgaatct ctcgatacat ttctgaatat gccaacctcg gtagctttcc ttgaaataag
    19981 taataagccc tatttgggga atatgttcaa cgatttcgct ggcgtggatc agcaacgagt
    20041 tatgcattgt cgtaaagcat ttagtgatgc gaaggtattc aaatgattcc tgattattta
    20101 acttttattc gctttcagga taaacgaagt ctgatataca tttatgctat tggacttatt
    20161 ctgataggct tttattggaa aaatgcagga tttacttttc catcagagga tattggtgtc
    20221 gttagtggga ttctggccct ggtgctgtat aattttattt ttgatctcaa ggcgtactgg
    20281 gcttataaat gcgtcacgaa gaatatcgat ttttcgtggt ttaagaaaaa gcagaaccac
    20341 aaaatagaat tatttcttac gcaacctctg gtggcaggat ttctgtcgtt aatcatgttg
    20401 agtgcaatga gttgggggct ataccagcgt ctaccctcgt tatatgcgct gttcctgatt
    20461 tcgttacttg ggccgttggt tatctttctg ctgtttcgga tgatccgcac cagttatgtc
    20521 aagcaggtcg ctatttcagt agcgaaaaaa gtaaaatata aaagtctgac tcgctatgtg
    20581 ctgctttcgg tgtgcatctc aacggttgtt aacctgctta ctatcagccc gttgcgtaac
    20641 agtgattctt ttgtgataga ggggcagtgg ttaacgttta aatcgataat tgcattgctc
    20701 attctttgtg gcgtagtgtt ggcgattaat ctgttttttc tgcgcttctc caggcggtac
    20761 gcttttttgg gcaggctttt tttgcaggaa atcgatctct ttttctccag tgaaaatgca
    20821 ttgtcgacct tttttgccaa gccgctttgg cttcggttat tcatattgct ggttattgaa
    20881 gtgatgtgga ttacgctggt gtcggtattg gcaacgcttg tggaatggcg gatttggttt
    20941 gaagcctatt ttttactctg ctatgtaccg tgcttaattt actatttttt ctattgtcga
    21001 ttcctctggc ataacgattt tatgatggca tgtgacatgt atttccgttg ggggcatttt
    21061 aataagtgag gaagtgatag gaagtgacca gataatacat atatgttctg tactctcttg
    21121 cgcattttga ttgttgactg agcaaccaga cagttgatgt gcacgatttc ccctcgccca
    21181 aacagacgtg ggcgggggca cccggtgtgt gcaatatacg aaaaaaaagc ccgtactttc
    21241 gtacgagctc ttctttaaat atggcggtga gggggaccgc ctgaaataaa tctgacttaa
    21301 tccaggggtg ttatataatt tcattataaa caatgtatta cactttttgt tgtccgatat
    21361 tatctaatgt attcttagtg aagcgagaga ctaatggcat actggatggc ataccagagt
    21421 gacgggaatt agcgtatggc agtattgacg gatacgaaag caagacatat caaacctgat
    21481 gacaaaccat tgccccatgg gggaattacc ggactgaccc ttcatccttc ttcagtaaag
    21541 gggcggggga aatgggtttt tcgttatgta agtccggtga cacaaaaaag acgtaatgct
    21601 ggattgggaa cttacccaga ggtcagtatt gctgaagctg cacgtactgc ccggataatg
    21661 cgagagcaac ttgctgcagg tgatgatcct ctggagatta aaaaggctga atctgagaaa
    21721 gtcgctatcc caacatttgc cgatgcagcc aggcgtgtac atgcagaact gtctcctgga
    21781 tgggaaaatc caaagcatgt aaggcagtgg ttatcgacgc ttgagaatta cgcgtttcct
    21841 caactgggag caaaaacgct ggattcgatt acggctgcgg acgtggcaga aacactgcgt
    21901 ccagtctggt taaccttgtc agaaacggca agccgggtta aacagcgcat tcatgttgtt
    21961 atgcagtggg gatgggcgca cggtttttgt gtagcaaatc ctgttgatgt ggttgaccat
    22021 ttgcttcctc agcagacaag aggacgtgat gaacaccaac ccgcaatgcc ctggaggcag
    22081 ttaccgcttt ttgtggcgac cagtgtgtat accgatgaac cttataatgt tacccgcgca
    22141 ctgttattaa tggtgatact tacagcaact cgctcgggcg aagccagggg aatgcgctgg
    22201 gctgaaattg attttcataa gcgggtatgg actatacctg cagaaagaat gaaagccagg
    22261 atacagcatc gtgttccttt atcccggcag gctatttaca ttctggaaaa tatacgtggc
    22321 ctgcatgatg aactggtgtt cccttcaccc agaaagcagc agatcctttc cgatatggtg
    22381 ttgacaagtt ttctgcgtaa aaagaaagcc gtcagtgaca ttccggggcg agttgccacg
    22441 gcacatggtt ttcgctcaac attcagggac tggtgtagcg aacaggggta ttcgcgggat
    22501 ctggcggaaa gggcgctcgc tcatacgctg aaaaataagg ttgaggcggc atatcatcgt
    22561 actgatctac tggagcagcg tgtaccgatg atgcaggcat gggcggatta tgtgatgtct
    22621 caaattgtga ataaataata gtgaaaatgt atattgtacg atcatgattt gatatgtgat
    22681 cgtagcagta gtaattaaga aaccgctttt atgtcaaatg tattgagctg ataattatca
    22741 gatcgtcagg aaagcgtatt ttgattaaca ggggattact taacaatccc ct
//
LOCUS       DAZFDC010000073        22786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFDC010000073 DAZFDC010000000
VERSION     DAZFDC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.073"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            247..2397
                     /gene="btsT"
                     /locus_tag="ACHJ48_002904"
     CDS             247..2397
                     /gene="btsT"
                     /locus_tag="ACHJ48_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO4708420.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2447..2650
                     /locus_tag="ACHJ48_002905"
     CDS             2447..2650
                     /locus_tag="ACHJ48_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO4708421.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2661..3617
                     /gene="yjiA"
                     /locus_tag="ACHJ48_002906"
     CDS             2661..3617
                     /gene="yjiA"
                     /locus_tag="ACHJ48_002906"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO4708422.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3686..5044)
                     /locus_tag="ACHJ48_002907"
     CDS             complement(3686..5044)
                     /locus_tag="ACHJ48_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFO4708423.1"
                     /translation="MQKRVLPIDVYSDETDEPRLIVRKKEHDLYKYYILQDSKDYKPE
                     KPSDTELVFISNAETIRDYLLRLSVDELKLLAKYILQNVYIVFVQTDDFASSFRLFNV
                     LNSRGLPLSNADLLKNALFESASTHNKKSEQIESAWSQIEDMVGVRRLDKFLTLHKLS
                     EKKDRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFEHPSIRRK
                     IASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMHGWLKKQIKSQR
                     EMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPRPNQVNLIKAILLR
                     LDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGNLTLISGS
                     KNSEAQHYDFIKKKSIYEKLNSKSSFDLTKDVCNSSEWGLAELKMRHEKMKTQLKKLW
                     LV"
     gene            complement(5053..5337)
                     /locus_tag="ACHJ48_002908"
     CDS             complement(5053..5337)
                     /locus_tag="ACHJ48_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO4708424.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENGYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKSSRFRG"
     gene            5615..8047
                     /gene="hsdR"
                     /locus_tag="ACHJ48_002909"
     CDS             5615..8047
                     /gene="hsdR"
                     /locus_tag="ACHJ48_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO4708425.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVKDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            8114..9583
                     /locus_tag="ACHJ48_002910"
     CDS             8114..9583
                     /locus_tag="ACHJ48_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO4708426.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPAELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            9583..11337
                     /locus_tag="ACHJ48_002911"
     CDS             9583..11337
                     /locus_tag="ACHJ48_002911"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO4708427.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
                     QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFV
                     CYVENCQTGVAYPAINDKQFFSGITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSL
                     DAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVDALKQTILQLAVMG
                     KLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCL
                     FEDIIDIQSGITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQV
                     VKGDLLITEGGDWDTVGRTTVWCHDWYIANQNHVFKGRNIGQDVDPYWLETYMNSPFS
                     RQYFANASKQTTNLASINKTQLRGCPVAIPPSSEAKKIMSKLHIFYKLCEELKNHIQS
                     AQQTQLHLADALTDAAVN"
     gene            11558..11899
                     /gene="symE"
                     /locus_tag="ACHJ48_002912"
     CDS             11558..11899
                     /gene="symE"
                     /locus_tag="ACHJ48_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO4708428.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(11946..13109)
                     /locus_tag="ACHJ48_002913"
     CDS             complement(11946..13109)
                     /locus_tag="ACHJ48_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO4708429.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(13157..14038)
                     /locus_tag="ACHJ48_002914"
     CDS             complement(13157..14038)
                     /locus_tag="ACHJ48_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO4708430.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVACGQQGYSEYFDSFMRHYLTVKTGRSLGQMKS
                     MRHLNSMPAARVLLKKA"
     gene            complement(14135..14299)
                     /locus_tag="ACHJ48_002915"
     CDS             complement(14135..14299)
                     /locus_tag="ACHJ48_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO4708431.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            14476..15888
                     /locus_tag="ACHJ48_002916"
     CDS             14476..15888
                     /locus_tag="ACHJ48_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO4708432.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDLVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVTRQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(16131..17088)
                     /locus_tag="ACHJ48_002917"
                     /pseudo
     CDS             complement(16131..17088)
                     /locus_tag="ACHJ48_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            17555..18787
                     /gene="mdtM"
                     /locus_tag="ACHJ48_002918"
     CDS             17555..18787
                     /gene="mdtM"
                     /locus_tag="ACHJ48_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO4708433.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRKPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            18828..20099
                     /locus_tag="ACHJ48_002919"
     CDS             18828..20099
                     /locus_tag="ACHJ48_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO4708434.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQKKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMTARADAVKSYLKEDEAFLSELWGDLREWLKADINSEDSRVKERIARAGQWFGETLI
                     ADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKDLQF
                     IRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            20215..21366
                     /locus_tag="ACHJ48_002920"
     CDS             20215..21366
                     /locus_tag="ACHJ48_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO4708435.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKVRGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQFNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            21376..22143
                     /gene="yjiL"
                     /locus_tag="ACHJ48_002921"
     CDS             21376..22143
                     /gene="yjiL"
                     /locus_tag="ACHJ48_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO4708436.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNTHPDAQFAGTIGAAVIGQRVRTRR"
     gene            22140..22406
                     /locus_tag="ACHJ48_002922"
     CDS             22140..22406
                     /locus_tag="ACHJ48_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO4708437.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
BASE COUNT         5857 a         5610 c         5448 g         5871 t
ORIGIN      
        1 gctaaaaatc gcttcggttg tgatcatcat cacataaata accttcataa accttgctca
       61 aaaccgctca accgcaaaac tgaccgctta cgtccctaaa ataaccactc agttatttac
      121 cttactttac gcgcgcgtaa ctctggcagc atcattgcag gatagcggtc aatttacctc
      181 ctcaaacgca acgcaaacct agaacggctt cggccaacta ttaatcaata catgccaggt
      241 tttactatgg atacgaaaaa actattcaag cacataccct gggtgattct cggaatcatc
      301 ggtgcattct gcctcgcggt agttgcatta cgtcgggggg agcacgtcag cgccctgtgg
      361 atcgtggtcg cctctgtgtc ggtgtatctg gtggcgtatc gctactacag tctgtacatc
      421 gcccagaagg tgatgaaact cgaccccacg cgcgcgacgc ctgcggttat taacaacgac
      481 ggtctgaact acgttccgac caaccgttac gtgttgtttg gtcaccactt cgccgctatc
      541 gccggtgctg gtccgctggt gggaccggtt ctcgccgcgc agatgggcta cctgcctggc
      601 acgctgtggc tgctggcggg ggtagtgctg gccggtgcgg ttcaggactt tatggtgctg
      661 tttatctcct ctcgccgtaa cggcgcatct ctcggtgaga tgatcaaaga agagatgggg
      721 ccagtaccgg ggactatcgc gctgtttggc tgtttcttaa tcatgatcat catcctcgcc
      781 gtcctggcgc tgattgtggt taaagccctg gcagaaagtc cgtggggtgt cttcaccgtt
      841 tgctcaaccg taccaattgc gctgtttatg ggtatctaca tgcgctttat ccgtccgggg
      901 cgtgtgggtg aagtctctgt tattggtatc gtgctgctgg ttgcctctat ctacttcggt
      961 ggcgtgattg ctcacgatcc gtactggggt ccggcactga cctttaaaga caccaccatt
     1021 accttcgcgc tgattggcta tgcgtttgtt tccgcgctgc tgccggtgtg gctgatcctc
     1081 gcaccacgcg actatctggc aaccttcctg aagatcggcg ttatcgtcgg cctggcgctg
     1141 ggtatcgtgg tgctgaaccc ggaactgaaa atgcctgcga tgacccagta cattgacggt
     1201 actggcccgc tgtggaaagg cgctctgttc ccgttcctgt tcatcaccat cgcctgtggt
     1261 gcggtatctg gcttccacgc gctgatctct tccggtacga cgccgaaact gctggctaac
     1321 gaaaccgacg cgcgtttcat cggctacggc gcaatgctga tggagtcctt cgtggcgatt
     1381 atggcgctgg ttgctgcatc catcatcgaa ccgggtcttt acttcgcgat gaacaccccg
     1441 cccgcgggcc ttggcatcac catgcctaac ctgcatgaaa tgggtggcga gaacgcgccg
     1501 atcatcatgg cgcagctgaa agacgttacc gcacacgcgg cagcgaccgt cagctcttgg
     1561 ggcttcgtga tttcgccaga gcagatcctg caaaccgcga aagacattgg tgagccttct
     1621 gtcctgaacc gtgcaggtgg tgcgccgacg ctggcggtag gtatcgctca tgtgttccac
     1681 aaagtgctgc cgatggctga catgggcttc tggtatcact tcggtattct gttcgaagcc
     1741 ctgttcatcc tgaccgcgct ggatgcgggt acccgttctg gccgctttat gctgcaagac
     1801 ctgctgggta acttcatccc gttcctgaaa aaaaccgact ctctggttgc tggtatcatc
     1861 ggtactgcgg gctgtgtggg tctgtggggc tacctgctgt atcagggcgt ggtcgatccg
     1921 ctgggcggcg ttaagagcct gtggccgctg ttcggtatct ccaaccagat gctggcagcc
     1981 gtagcgctgg tactgggcac cgttgtgctg attaagatga agcgcaccca atacatctgg
     2041 gtaactgttg ttccggctgt atggctgctt atctgcacca cctgggcgct gggtctgaaa
     2101 ctgttcagca ccaacccgca gatggaaggc ttcttctaca tggcaagcca gtacaaagag
     2161 aagattgcta acggtactga ccttacggcg cagcagattg ccaacatgaa ccacatcgtt
     2221 gtgaacaact acaccaacgc aggcctgagt attctgttcc tgattgtggt gtacagcatc
     2281 atcttctacg gtttcaaaac ctggcttgcg gtacgtaaca gcgacaaacg tactgacaaa
     2341 gaaacaccgt acgttccaat cccggaaggc ggcgtgaaga tctcttcgca ccactaaccg
     2401 tgtttagccc cgcttcggcg gggctttgtt cttatcagag tgaactatgt ttggtaactt
     2461 aggacaggca aaaaaatatc tcggccaggc ggcgaagatg ttgattggca ttccagacta
     2521 cgacaactac gtcgagcata tgaagaccaa ccatcccgac aagccgtaca tgagctatga
     2581 agaattcttc cgcgaacgcc agaacgcgcg ctacggcggc gatggtaaag gcggtatgcg
     2641 ctgttgttaa tggagagagc atgaacccga ttgcagttac cctactcacc ggttttttag
     2701 gcgcgggcaa aaccaccctg ctgcgccata ttcttaacga acaacacggc tacaagattg
     2761 ccgtgattga aaacgaattt ggcgaagtct ccgttgatga tcaactgatt ggcgaccgcg
     2821 ccacgcagat caaaacgctg accaacggct gcatctgctg ttcgcgctcc aacgagctgg
     2881 aagacgcact actcgacctg ctggataatc tcgataaggg caatattcag ttcgatcgtc
     2941 tggtcattga atgcaccggc atggccgatc ccggcccgat tattcagacc tttttctccc
     3001 atgagatttt atgccagcgt tacctgctgg acggcgtgat tgcgctggtg gatgcggtac
     3061 atgccgatga gcagatgaac cagttcacca tcgcccagtc ccaggttggc tacgccgacc
     3121 gtattctgct gaccaaaacc gacgtcgcag gcgaagcaga aaaactgcgc gaacgcctgg
     3181 cgcgcatcaa cgcccgtgca ccggtctaca ccgttaccca cggcgacatc gacctgggcc
     3241 tgctgttcaa caccaacggt tttatgctgg aagaaaacgt cgtcagcacc aaaccgcgtt
     3301 tccactttat cgcggataaa caaaacgata tttcgtcgat tgtggtagaa ctggattacc
     3361 cggtagatat cagcgaagtt tcccgcgtga tggaaaacct gctgctggaa tcggcggata
     3421 aactgctgcg ttacaaaggg atgctgtgga ttgacggcga acctaaccgc ctgctgttcc
     3481 agggcgtcca gcgcctctac agcgccgact gggaccgccc gtggggcgat gaaaaaccgc
     3541 atagcacaat ggtttttatc gggattcagt tgccggagga agagattcgg gcagcgtttg
     3601 cggggttgag gaagtaagtt cgggaaagat caactttttg agagttccac cgcaacatta
     3661 ttacgagtgg aactctttag atatgtcaga ccaaccaaag cttcttcaat tgcgttttca
     3721 ttttttcatg tctcattttc agttcagcca atccccattc tgaagaatta caaacatctt
     3781 ttgttaaatc aaaagagctt ttactattaa gtttctcata aatagatttc tttttgataa
     3841 aatcatagtg ttgtgcttca gaatttttgg aaccacttat gagtgttaaa ttacctattt
     3901 tatgcagcca gtagacatgc tcttgaggtt ggaatctatt aatccagtat tcattaacta
     3961 atgcttgagg aagaatatgc tcaattgtga ttctccccgt ataggtttta atcacgctct
     4021 catcttgttg ctccatatct aagcgaagca aaattgcttt tattaagttc acttgattcg
     4081 gacgaggctc atataaatct tcatccagag cagctataaa acctgaatta tctgcatgtt
     4141 ggtttatctg atttatcaca gaatcaaaag gcatatcatt atttattgct accagagctg
     4201 aataacaaac catttctctt tggcttttaa tttgtttttt taaccaccca tgcatatata
     4261 ccttctcaaa ggcggtaata aattgagaaa aatcatctaa attaaaatct tctgtacgag
     4321 ccattcgatt catgaatgcc ataaccggag gaatccactc atctacccct aaattactta
     4381 gcgatgctat ttttcgtcgt attgaagggt gttcaaaatc attctctaaa atcttagtat
     4441 aattttttgc agaattaacc aacatcagtg acatagcaat agcatctcca tcaaattgct
     4501 gctggagatt ttcaataaaa gcttcaaaac ctttttgtaa caccctgtca cgatcttttt
     4561 tctcggaaag cttatgaaga gtaagaaatt tatcaagccg tctcactccg accatatctt
     4621 caatttgcga ccatgctgat tctatttgct ctgatttttt attatgtgta gaagctgatt
     4681 caaacagtgc attttttaaa agatctgcat tacttaaagg taaacctcga ctatttaata
     4741 cattaaagag tctaaatgat gatgcgaaat catctgtttg cacaaatacg atatatacat
     4801 tttgcaaaat atatttagca agcaacttca actcatcaac agataaccgc aacaaataat
     4861 ctctaattgt ttcagcgttg ctaatgaata ctaactcagt atccgatggc ttttcaggtt
     4921 tataatcttt agaatcctgc agtatataat atttataaag atcatgctct ttttttctaa
     4981 caattagtct tggttcatct gtttcatcag aataaacatc aataggcaga actctttttt
     5041 gcaaatcatc ttttatcccc tgaatcggga tgattttttt agctcggaga caataatgct
     5101 taacgttgta agacgttgtt gtccatctac aacttcatac tgattttgcc ctttattgat
     5161 acaaatcata ctcccaataa aatatccatt ctcttctgat tgagagcttt tataaatgtc
     5221 atcaatgagt tgctctgcat tatctaccgt ccaactgtaa ggacgctgat aagaaggaat
     5281 aataaacttt ttgttttcag taaggatatc ctgcacctta cattctttag cttccatttc
     5341 atatacccaa tcaaacaatc aacaataaca ttatctttct tgctcgaaag aaacgcaact
     5401 ataagatttt tatatatttt gttagagttg atatggttcc tgtaacttct gtttatctgt
     5461 ctctgatatg cccagattaa ttcaagcaag atataacaga gatatcaatg tgatctctgc
     5521 tacgtacttt acgttgaaac accgccctaa atcatagcca ggctattgat tttatgtaca
     5581 atcagaatcg tcctgataaa agcgagaata ccccatggcg gaactgaacc taagtaacct
     5641 gacggaagca gacatcatta ccaaatgcgt tatgccagcc attctcaatg caggctggga
     5701 caacacaacg caaatcagac aggaggtcaa actccgggac ggtaaagtca tcgtacgtgg
     5761 taaagttgcg gcacgcagaa cggtaaaatc tgctgacatc gtgctgtatc acaaacctgg
     5821 cattccctta gccgtgattg aagcaaaagc caacaaacat gaaattggca aagggatgca
     5881 acagggcatt gaatatgcgc gcctgctgga cgttcctttt gtttttgcca ccaacggtga
     5941 tggctttatc ttccgcgatg ccaccgcagc cgaaggtgaa tgccttgaaa agcaaataac
     6001 gctggatgac ttcccctccc ccgctgaact ctggcaaaaa ttctgcctct ggaaaggata
     6061 cacacaagct cagcttccgg tgattactca ggactattac gacgatggca gcggtaaatc
     6121 gccacgttat taccagcttc aggcaatcaa caaaaccatt gaagccgtct ccaacgggca
     6181 aaaccgcgtt ctgctggtca tggcgaccgg aacagggaaa acctataccg cattccagat
     6241 catctggcgc ctgtggaaat caaaaaataa aaaacgcatt ttgttccttg ccgatcgcaa
     6301 tattctggtc gaccaaacca aaaataatga tttccagcca tttggtacgg caatgaccaa
     6361 agtcagcgga cgcaccattg atcccgctta tgaaattcac ctcgcgctct atcaggctat
     6421 aactggcccg gaggaagacc aaaaagcgtt taaacaagtc gcaccagatt tcttcgatct
     6481 gatcgtgatc gacgaatgcc atcgcggcag cgcatccgaa gacagcgcct ggcgagaaat
     6541 ccttgattat tttagttccg ccacccaaat tggcttaacc gccacgccaa aagagacgca
     6601 tgaagtctcc agcacggatt acttcggcga tccggtttac gtctactcac taaaagaagg
     6661 gatcgaagac ggcttcctcg ccccttataa agttgtccgt gttgatattg atgttgatct
     6721 gcaaggctgg cgcccaacca aagggcaaac tgacttaaac ggcgaagtga tcgacgatcg
     6781 tatctataac cagaaagatt tcgatcgcac gatggtaatc gacgaacgca cagaactggt
     6841 tgccagaacc attaccgact acctcaagcg taccaatccg atggataaaa ccatcgtctt
     6901 ctgtaacgac atcgatcatg cagaacgtat gcgccgcgcc ctggttaatc tcaacccgga
     6961 gcaggtgaaa aagaacgaca aatacgtcat gaaaatcacc ggcgatgatg aaattggcaa
     7021 agctcagttg gataacttca tcaacccgaa aaaaccgtac ccggttatcg cgaccacttc
     7081 agagctgatg accaccggtg tggatgctaa aacctgcaaa ctggtagtac tggaccagaa
     7141 catccagtcg atgaccaaat tcaagcagat tatcggtcgt ggtacacgca tcgacgaacg
     7201 ttacggcaaa ctctggttta ccatcctcga ctttaaaaaa gccaccgaac tgtttgccga
     7261 tgagcgtttc gatggcattc ccgaaaaagt catggatacc acaccagagg atatcgccga
     7321 tccagaatct gattttgaag agaaactcga agaaatcagc gaacatgacg acgaacaggt
     7381 aacaggcgtt gatgaaccgc ctgcgccacc ataccaggtt aaagataccg atgatgtcgg
     7441 cccacttccg gaagaagacg agaagaaaat ccgcaagttt cacgtcaacg gtgtagcagt
     7501 gggcgttatt gcccagcgtg ttcagtatta cgacgccgac ggtaaactgg ttaccgaatc
     7561 ctttaaagat tacacccgca aaacactgct caaagaatat gcctcgctgg atgactttac
     7621 ccgcaagtgg caggacgccg atcgcaaaga agcgatcatt cacgagctgg agcaacaggg
     7681 gatcatctgg gaagtactgg cagaagaagt cggtaaagat ctcgacccgt tcgacatgct
     7741 ttgccacgta gtgtatggtc agccgccgtt aacccgcaaa gagcgcgccg agaacgtgcg
     7801 caagcggaac tacttcacaa aatactctga agcagcgcaa gccgtgctcg ataatctgct
     7861 ggataaatac gccgatgcgg gcgtacagga gatcgaaagt attcaggtgc tgaaacttaa
     7921 gccattcgac agcatgggca ccttaccgga gattattaaa accggatttg gcgaccgtaa
     7981 cgggtataat caggcgctca gcgagctgga aaacgaaatc taccaattac cgccccgctc
     8041 tgcttaacaa ctctcttttg cgccttctgc aaccatcatg ttgcagaagg ccttgttttt
     8101 catggaatac aacatgtcta tcagctcagt aatcaaatca ttacaagata ttatgcgcaa
     8161 agatgccggt gtggatggcg atgcgcagcg tctcggtcag ctctcctggc tgctgttttt
     8221 gaaaatcttc gatgcccagg aagaggcgct ggaactggag caggataact atcaatatcc
     8281 gatcccacag cgttatttat ggcgcagttg ggccgccaac gcgcagggca ttaccggtga
     8341 ttccctgctg gaattcgtta atgatgatct gttcccggcg ttgaaaaacc tcactgcgcc
     8401 tatcgataaa aacccacgcg gctacgtggt gaagcaggcg ttcagcgatg cctataacta
     8461 tatgaaaaac ggtacgctac tgcgtcaggt gatcaataag ctgaacgaaa ttgactttac
     8521 cagcgccagc gaacgccatc tgtttggtga tatttacgaa cagatcctta aagatctgca
     8581 atctgcgggc aatgcgggcg aattctatac tccacgcgcc gtcactcgct ttatggtgga
     8641 tcgcgttgat ccgaaactcg gcgaatccat tatggacccg gcctgcggta cgggcggttt
     8701 tcttgcctgc gcatttgatc atgtaaagaa caaatacgtg aagagcgtcg ccgatcatca
     8761 gacgctgcaa caacagatcc acggtgttga gaaaaaacag cttccgcacc tgctggcgac
     8821 caccaatatg ctgctacacg gcattgaagt gccagtgcaa attcgtcacg acaacactct
     8881 gaacaaaccg ctttcctccc gggatgagca actggatgtc attgttacca acccgccgtt
     8941 tggtggcacg gaagaagacg gtattgagaa gaactttccg gcagagatgc aaacccgcga
     9001 aacggcggat ttgttcctgc aactgattgt ggaagtactg gcgaaaaatg gtcgcgcggc
     9061 ggtggtattg ccggatggca cactatttgg cgaaggcgtt aaaaccaaaa tcaaaaagct
     9121 acttaccgaa gagtgcaacc tgcataccat cgtgcgctta ccgaatggtg tgtttaaccc
     9181 ctataccggc attaaaacca acctgttgtt ctttaccaaa ggtcagccaa ccaaagagat
     9241 ttggttctat gagcatccgt atccggcggg cgtaaaaaac tacagcaaaa ctaagccgat
     9301 gaagtttgaa gagtttcagg cggagatcga ctggtggggt aacgaggccg atggttttgc
     9361 cagccgcgta gagaatgagc aggcgtggaa agtcagcatt gatgacgtga ttgcgcgtaa
     9421 cttcaatctg gatattaaaa acccacatca ggcggaaacc gtcagccatg atccggccga
     9481 gctgttagcg cagtatgcaa aacagcaggc ggagatccag acgctgcgta atcaactgcg
     9541 cgatattctt ggcgctgcgc tgtctggcaa ggaggttaac taatgagtgt ggaaaagctg
     9601 atcgttgatc atatggaaac ctggacctcg gcgttgcaaa cccgttccac cgccgggcgc
     9661 ggcagttccg gaaaaattga tttatatggc attaagaaat tacgtgagct gattctggaa
     9721 ctggctgtgc gcggtaaact ggtgccgcag gacccgaacg atgaaccggc gtcggagctg
     9781 ctgaagcgta ttgcggcaga aaaagcagag ctggtgaagc aaggtaaaat taaaaagcaa
     9841 aaaccactgc cggaaattag tgaggaagag aagccgtttg aattgccgga gggatgggag
     9901 tgggtgcgta ttagtgaaat tggacatgac tggggacaaa aaactccaga taaagatttt
     9961 acttacattg atgttggttc aataaataaa gaatatggaa ttattgaaga gctatctatt
    10021 ctttcagcga aagatgcccc atcgcgagcg agaaaaattg ttcagcaagg gactatcata
    10081 tactctactg ttcgcccgta tttgttaaat atagctatta ttgaaaacga aatactacct
    10141 gaaccgatcg ctagtactgc cttcgctatt attcatccat atacggcgat ggatgctaat
    10201 ttcatttatt attatctacg ttctccggta tttgtttgtt atgttgaaaa ttgtcagaca
    10261 ggggttgcct atccagcaat caatgacaaa caattttttt ctggaataac tccagttcct
    10321 ccatccttgg aacaagttcg catcgcaaac aaaatcaaag aattaatgtc cctctgcgac
    10381 caactggaac agcaatccct gaccagtctg gacgcacatc aacaactggt tgaaaccctg
    10441 ttgggaacgc ttacagacag ccaaaccgcc gaggaactgg ctgaaaactg ggcgcgtatt
    10501 agcgagtatt tcgacactct atttaccacc gaagccagcg tggatgcgtt aaaacagacc
    10561 attctgcaac tggccgtaat gggcaaactt gtgccgcagg atccgaatga tgaaccagcc
    10621 tctgaactgc tcaaacgaat tgcgcaggaa aaagctcaac tggtgaaaga aggaaaaata
    10681 aaaaaacaaa aaccgttgcc gccaattagc gatgaggaaa aaccgtttga acttccggaa
    10741 gggtgggagt ggtgtttatt tgaagatatt attgatattc aaagtggtat cactaaagga
    10801 agaaatttat caaatagaac tttggtaaaa gttccttatt tacgtgttgc aaacgtccaa
    10861 cgcggatatc ttgatcttac ggaaattaaa cagattgaaa tccctattga ggaaaaagaa
    10921 aaatatcaag tagtcaaggg agatttattg ataacagaag gcggcgactg ggatacagtc
    10981 gggagaacta cagtatggtg tcatgactgg tatatagcaa atcaaaacca tgtattcaaa
    11041 ggacgaaata tagggcaaga tgttgatcca tattggttag aaacatatat gaatagccca
    11101 ttctcaagac aatattttgc taacgcaagt aagcaaacca ctaatttagc ttctattaat
    11161 aaaacccagc tcagaggttg tcctgttgct attcctccta gctcagaagc aaaaaaaata
    11221 atgagtaaac tacatatttt ttataaacta tgtgaagaat taaagaatca tatccaatcc
    11281 gcccagcaaa cccaactaca ccttgcagat gcactcactg acgcggcggt aaactaattt
    11341 acctgccctc gaaaaaccaa taaagcggct aaatagccgc tttattcaca tcaccaaaat
    11401 ttatatttca cgctaatttt catcttattt tcattaattt ttagctggac tttccccata
    11461 tttactgatg atatatccag gtatttagcg cggtgcggat gtgcgccaac acacccgcac
    11521 cgctaatcac aatcactatt tatagggaat agcagttatg actgatacgc attctattgc
    11581 acaaccgttc gaagcagaag tctccccggc aaataaccgt caattaaccg tgagttatgc
    11641 gagtcgttac ccggattaca gccgtattcc cgccatcacc ctgaaaggtc agtggctgga
    11701 agccgccggt tttgccaccg gcacggtggt agatgtcaaa gtgatggaag gctgtattgt
    11761 cctcaccgcc caaccacccg ccgccgccga gagcgaactg atgcagtcgc tgcgccaggt
    11821 gtgcaagctg tcggcgcgta aacaaaggca ggtgcaggag tttattgggg tgattgcggg
    11881 taaacagaaa gttgcctgat acattgaccc ggtagcgcca ggactaccgg gcatttttcg
    11941 ctgcgttata ccaacgcgct ctccatctgt ttttcaaatg cctgacgaat taaccccata
    12001 atgtaaggaa gttgtgcgag gtcactgaaa ccaatttcca catcaccatt gccccatcgc
    12061 ccaacatttg tcacatcttt ggcaatacct ttcggatcga ccagttcatg aaactgcata
    12121 ttaagcgaca atcgcagtcg gcttttttgc ggcacaacat caacaaaatt cgtttccgct
    12181 ttaaacgcaa tatagagctt caatacttcc tgcgtgatcc ctgcgtccag gcgcataatt
    12241 tcatcgcgaa gatgatcgaa taacacccgg ctatgcgaac catcagcaag aaaaggataa
    12301 tcactcaggc tgtaactggt tttgttctca ggctgtcccc gataaacagc taaaacctct
    12361 tcaggaagag aaggcgcttg ccagacctga accgcaagct ccgccagacg atcggctcgg
    12421 gcgtgaatgg cagcttcatc ccatttttcg cactgtccaa gaccgatatt caaatagagc
    12481 gggctatgct taaagccgtc ttcaatatcg cgtttttctg cgaaaggtct gtcactgtaa
    12541 cgggagttat agcgcgttaa agtgagattc cccaacgtat gcaacagttc tttatgaata
    12601 cgctgccagt cacttcccag ctcttcgcgc catttagccg acaaattttc gttctgcggc
    12661 ataatatgtt caatcgtaaa ctcttccacg cgctctctgc gtttatcgtt ttccagtcgt
    12721 cgtaaccagt agctacgact acggaagtta tagagatcgc gaacttttaa ttcccgttta
    12781 aattcatcat cgttggggaa acgacgatat gaaggtagat tcaaaaaatg tacctgaata
    12841 ctttccagat atttttcttt attaatgacc ttataaaaag tagcaaacgt cttgttaaga
    12901 gaatttgtcg gaatcgcaca tactgcacgg cggaaaacat aagattcaat taaacgaatt
    12961 atgctcagga aatcttcgtg agacaaatcg tcatttttat agtcatgata aagcgccagt
    13021 aagaaaggat acgccacatc aacctttaac tcgcgcaaat cctgaaaagc cgtagcaagc
    13081 gatttgtcac tttcttttcc caatgccatt gcacagtaat actccgcgta gatatgaata
    13141 tcttcaacca gccgatctac gcctttttca gcaacactct ggctgcgggc atggagttta
    13201 aatgcctcat agacttcatc tgtccgaggg atctccccgt ttttaccgtc agataatgac
    13261 gcataaaact gtcaaaatat tcgctgtaac cttgctgacc acaagcgact tccattggac
    13321 gccagtgatc ttcatacaac cgggtttgat gctctggttc gaggcccatc agaataaaat
    13381 tgcgcaccag atcggcctga cttaacgcct taccggtgga gttcatactt tcaaaaatca
    13441 gttgcggatt atcctgacca cgattaagcg ccacatcgac aattaataac tttgctaacc
    13501 cacgacataa ggggatcaag tcatcaccga gtttggcaat ttgttcatca aagaaagtaa
    13561 agttttccat tattcgatgg gaatagtttt ctggcattgg tctttgtttt attaacgcca
    13621 gtaaactgtc tttatcagtc tcggtcagca gtaatttaaa acctttctcg ccggactcat
    13681 aaggattgag caaataataa ttacgcaatt tcattgccga aaagccatca aaaacttcgt
    13741 cttcgccaag atggcgcgat aaagcctcaa tcagcaacat tgcggtcgtc agccgctgct
    13801 gaccatcaat gaccagcaac ggagaaatac cagaaacctg atacaatccc tgctctatat
    13861 aaacaaccga accgataaaa tgcgctgata tatcgtcacg ctttcctgca cgaatgatgt
    13921 cgtcccaaag ttgccgacat tgttgttctg tccatgaata agtccgttga taaatgggaa
    13981 taacaaactg ctgcgaacgt tttaaaaaat cgagtaggcg agcttctgta gctttcattc
    14041 tgacgtcttt ccgtgaaacg atgatttcgc aataataacc acttccttat ggaggttata
    14101 gagaaatcct gtttatccgc gacgaacata cccctcactc cacatcctcc ctgcgtaacc
    14161 caatatccct caaccgttca tcactcatct gctgtaacac ccgtcgggcc tgcatttgcc
    14221 gccgccaacg cctcactgcc tgccagagtt gcacaagtct gataaacggc gttttggctt
    14281 tgttttcgtg aaattccatc tgctgcctcc tcatatttca gaggcttcat catccgtctt
    14341 ctggcagttc acaatacaga tgcaaatatt agttttgttt aacatacaga caggttaaaa
    14401 cagcatctgc atccggtttt ttagcctcat ctgtaccggt ttttctttct gtatggtcca
    14461 aataaggaat acagcatgac gcgttatcaa catctggcga ctctacttgc cgagcggatt
    14521 gagcaagggc tgtatcgtca cggggagaaa ttgccgtcgg tgcgcagctt aagtcaggag
    14581 cacggcgtca gcatcagcac cgtgcagcag gcgtaccaga cgctggagac gatgaagctc
    14641 atcactccgc agccgcgttc gggttatttt gtcgcacaac gtaaagccca gccgccagtg
    14701 ccgccgatga cgcgtccggt gcagcgccca gtggaaatta cccagtggga tctggtgctg
    14761 gatatgctgg tggcgcatag cgacagttcc attgttccgt taagcaaaag cacgccggat
    14821 gtcgaaacgc ccagcctgaa accgctgtgg cgggagctaa gccgggtggt gcagcataat
    14881 ctgcaaaccg ttctcggtta tgacttgcta gccggtcagc gggtattgcg cgagcaggtt
    14941 gcccgcctga tgctcgacag cggctcggtg gtcactgccg atgacatcat catcaccagc
    15001 ggctgccata actcgatgtc gctagcgtta atggcggtgt gtaaaccggg cgatattgtc
    15061 gcggtcgaat ccccctgtta ttacggttca atgcagatgc tgcgcggcat gggcgtgaaa
    15121 gtgattgaaa tcccaaccga tccagaaaca ggcatcagcg ttgaagcgct ggaactggcg
    15181 ctggaacagt ggccgattaa aggcatcatt ctggtgccaa actgtaataa tccgctggga
    15241 tttattatgc cggacgcgcg caaacgggcc gttctctctc tcgctcagcg tcatgatatt
    15301 gtgatttttg aagatgatgt ctacggcgaa ctggcaacgg agtatccgcg cccgcggacc
    15361 attcattcct gggatatcga cgggcgagtg ctgttgtgca gctcgttcag taaaagtatt
    15421 gctccaggcc tgcgcgtggg ttgggtcgca ccggggcgtt atcacgataa actgatgcat
    15481 atgaaatacg ccatcagcag ctttaatgtg ccgtccacgc aaatggcggc ggcaacgttt
    15541 gtgctggaag gtcactatca tcgccatatc cggcggatgc ggcagatcta tcagcgcaat
    15601 ttggcgcttt atacctgctg gatacgggaa tattttccct gcgaaatctg tattacgcgc
    15661 ccgaaaggcg gatttttact gtggatagaa ttgcctgaac aggtcgatat ggtctgcgtc
    15721 acgcggcagc tgtgccgcat gaaaatccag gtggcggcag gctcgatttt ctcagcttcc
    15781 ggcaaatacc gtaactgcct gcgcatcaac tgtgctttgc cactcagcga aacttatcgc
    15841 gaagcgctga agcaaattgg cgaggccgtg tatcgggcaa tggaataaat tagaattagt
    15901 ggcgaagcac ctgatttatt ctcacgaatc gcctgtgact cgttttatct ttgcaggatc
    15961 atagccacgt tccggcatgg cttaggtaaa gtgcgctttg ttcatgccgg atgccatgtg
    16021 aacgccttac caggtctaca aaatctttaa agttcaatat attgaatggg ctttgtagac
    16081 ctgataagcg tagcacatca ggctgctttg cgtttgtcat ccgtctctgg ttagctcttg
    16141 agccatgaat agcgctcacg ctcaggcaag ggctgcctta atgcctgcat ttgctctgcc
    16201 gaaagtccgg taatcttctg tatccattca ggatctgccc cattctgcaa caacaatcga
    16261 agaatgcgct gttccccttc ttcccttccc aacagccatc catcattatg aattcgctct
    16321 gcaatcgtca ttatgaattc gctctgcaat cgtcattatt cgctctctgt gttgaggcat
    16381 tcgactggtt agttcactga taaactcatt aaaacgctct tcatcgccag tcagtaaaat
    16441 gtaatttaac agcgcagtta tctggctgtc attagcacac tctgtaacca gtaatactac
    16501 cagttgatcg ataagcccca tcagatcgcg ctggcgaata tgcttttgga tcaactccaa
    16561 cagggcgact ctgcggtgct gcacaatctc gtcgtccggc acgacagtga tatccaccag
    16621 cgggaacgcg gcgttataaa gcttccgtgc agtagtcggg tcggcaaatt cgtccaacca
    16681 gcacaaagac cagggataag gactacggct accgtgataa aacagcatcg ggatgaccag
    16741 cggtagtggc cggcgtttat catgctctat atggcgctgc atcactgcca tggaatagcg
    16801 catcaggcga aaggccatat gaatatcctc acggctctga tgttcaatca ccacatagat
    16861 atagccatcg ccttcccggg tctttaccga ccacagaata tcggagtgta gcgcccgcaa
    16921 tttttcatcg acaaagctgg cggattccag ttttaagctg tcgagatcgc acagttcacg
    16981 taaatctttg ggtaagtgga tctccataaa atcccgcgcg gtgtcagggt gcatgagaaa
    17041 agatttaaat aatgcgtcat gcggcgtgct ggtcgtgaag tttgtcatgg cgatccgtca
    17101 cctgttgata agatggcccg actttagcga ctaacaaaac cattctcacc cggcaatttc
    17161 tcgtttcccg aggcgctttc caggctattt ttgttcgtct gcaaacgata catttttccg
    17221 gaaactgact cgcgttttac atgtcctgta cccggcagat attccccctt ccaccgcgct
    17281 cttttgatta atcgcaaatt ttaattacac ttaaggtgta tattttctat gcaacccatc
    17341 aattcaagag gtgtaatgtg ctgatgacta tttgtaatcg ttatacattc tgacccgaag
    17401 tcagaaggta tttctctgtc tgtgtgttca caggcagtgt ggttgattac atgaaattca
    17461 gtacatttgt ccttctcgcc taccttttgt cattaccgac ggtattgaat ttcgttttcc
    17521 ccgttggggt tctccggaca aggagttgtt tgttatgcca cgtttttttg cccgccatgc
    17581 cgccacgctg tttttcccga tggcgttgat tttgtatgac tttgccgcct atctttcgac
    17641 ggacctgatc cagcctggga tcattaatgt ggtccgtgat tttaatgccg atgtcagtct
    17701 ggctccggct gccgtcagtc tctatctcgc tggaggcatg gcgttgcagt ggctgctggg
    17761 gccgctttcc gacaggattg gccgcaagcc agtgctgatt accggggcgc taatatttac
    17821 ccttgcctgc gctgcgacaa tgttcacaac gtctatgaca cagtttctta tcgcgcgtgc
    17881 aattcagggc accagtatct gttttattgc caccgttggt tatgtcacgg tgcaggaggc
    17941 gttcggccag acaaaaggga tcaagttgat ggcgattatc acctccatcg tactgattgc
    18001 gccgattatc ggcccgcttt ccggcgcagc tctgatgcac tttgtgcact ggaaagtcct
    18061 ttttgccatc attgcggtta tgggttttat ctcatttgtt ggcttactgc tggcgatgcc
    18121 agagacggtg aagcgcggcg cggttccatt tagcgccaaa agcgtcttgc gcgattttcg
    18181 taatgtcttt tgcaatcggc tgttcctctt tggcgcagca accatctctt taagctatat
    18241 ccccatgatg agttgggtgg ctgtctcgcc ggtgatcctg attgacgcag gcggcttaac
    18301 gacctcgcag ttcgcctgga cacaggttcc ggtgttcggc gcggtgattg tcgcgaatgc
    18361 catcgtggcg cgtttcgtta aagatccgac cgaaccgcgc tttatctggc gtgccgtacc
    18421 cattcaactg gtcggcctcg cgctgttgat tatcggcaat ctgctgtcac cgcacgtctg
    18481 gctgtggtcg gtgctgggca ccagtctgta tgctttcggg attggtttga tcttcccgac
    18541 cttattccgc tttacgctgt tttccaataa cttaccgaaa gggaccgtct ccgcatcgct
    18601 gaatatggtg atcctgatgg tgatgtctgt ctcggtcgaa attggccgct ggctgtggtt
    18661 taacggcggt cgcttgccgt ttcatctgtt agccgttgtg gcgggcgtta tcgtcgtttt
    18721 caccctggcc ggattgctta atcgcgtgcg ccaacatcag gctgccgagc tggcagagga
    18781 gcagtgattt ttgcgcgatc ctgccgtcag gctctattct taacgttatg aataaactca
    18841 ttgaactcag acgcgccaaa atgttggcgc tctctttact gcttatcgcc gctgctacct
    18901 ttgtcgttac gctgtttttg ccgcccaatt tttgggtgag cggcgtgaag gcgattgctg
    18961 aagcggcgat ggtcggcgcg ctggcggact ggtttgcggt ggtggcgctg tttcgccgcg
    19021 tgccgattcc gatcatttct cgtcatacgg cgattatccc gcgtaataaa gaccggattg
    19081 gcgaaaatct cggtcagttc gtgcagaaaa aatttctcga tacccaatcg ctggtggcat
    19141 tgattcgacg tcacgaaccg gcgttgctga ttggcaactg gtttagccag ccagaaaacg
    19201 cccgccgcgt tggtcagcat ctgttgcaga tcatgagtgg ttttcttgaa ctgaccgatg
    19261 atgcacgtat tcagcgcctg cttaagcgcg cggtccatcg ggcgattgat aaggtcgatc
    19321 tttccggcac cagtgcgttg atgctggaga gtatgaccaa aaacgatcgt catcaggtgc
    19381 tgctggatac gctgatcgca cagttgatcg cccttctcca gcgcgataaa tcgcgcaagt
    19441 ttatcgccca gcagattgtt cgctggctgg agagtgagca tccactgaaa gccaaaattc
    19501 tccccaccga atggctgggc gaacatagcg cggagttggt ttctgacgcg gtgaattctt
    19561 tgcttgatga tatcagccgc gatcgtgcgc atcagatccg ccatgcattt gatcgcgcca
    19621 cttttgccct gatcgacaag ttgaaaaacg atcctgaaat gacagcgcga gctgatgccg
    19681 taaaaagcta tctgaaagag gatgaagcct ttctcagtga attgtggggg gatttacggg
    19741 aatggctgaa agcggatatc aacagtgaag attctcgtgt gaaagaacgt atcgcgcggg
    19801 caggtcaatg gtttggcgaa acgttaattg ccgatgatgc cttgcgggcg tcgttaaatg
    19861 gtcacctgga acaagccgcg caccgcgtcg cgcctgagtt ttctgcattc ctgacgcgcc
    19921 atatcagcga tacggtaaaa agctgggatg cccgggatat gtcgcggcaa atagagttaa
    19981 atatcggcaa agatctgcag tttatccgtg tcaacggtac gctggttggc ggttgtattg
    20041 ggctaatttt atatttgctg tcacagctcc cggccttgtt ccccctcggc aatttttaga
    20101 aatccatgaa aaactaagtg gctaaccgcg agagagatca aataaaccat actaaaagaa
    20161 tggtaataat gtggcctctt ttcattatct cccgtggtac ggggaaggaa aatcatgtca
    20221 cttgtcaccg atctacccgc cattttcgat cagttctctg aagctcgcca gaaaggcttt
    20281 ctcaccgtca tggatctcaa ggtgcgcggc attccgctgg ttggcactta ctgcaccttt
    20341 atgccgcaag agatcccgat ggcagccggt gcggttgtgg tttcgctctg ttccacctct
    20401 gatgaaacca ttgaagaagc ggagaaagat ctgccgcgca acctctgccc gctgattaaa
    20461 agtagctacg gcttcggcaa aaccgataaa tgcccctact tctacttttc ggatctggtg
    20521 gtcggtgaaa ccacctgcga cggcaaaaag aaaatgtatg aatacatggc ggagtttaag
    20581 cccgttcatg tgatgcagct gccaaacagc gttaaagacg atgcctcgcg tgcgttatgg
    20641 aaagccgaga tgctgcgctt acaaaaagcg gtggaagaac gttttgggca cgaaattagc
    20701 gaagatgctc tgcgcgatgc cattgcgctg aaaaaccgcg aacgtcgcgc actggccaat
    20761 ttttatcatc ttgggcagtt caatcctccg gcgcttagcg gcagcgacat tctgaaagtg
    20821 gtttacggcg caaccttccg gttcgataaa gaggcgttga tcaatgaact ggacgcgatg
    20881 accgcccgca ttcgtcagca gtgggaagaa ggccagcgac tggacccgcg tccgcgcatt
    20941 ttaatcaccg gctgcccgat tggcggcgca gcagagaaag tggtgcgcgc gattgaagag
    21001 aatggcggct gggttgtcgg ttatgaaaac tgcaccgggg cgaaagcgac cgagcaatgc
    21061 gtggcagaaa cgggcgatgt ctacgacgcg ctggcggata aatatctggc gattggctgc
    21121 tcctgtgttt cgccgaacga tcagcgcctg aaaatgctca gccagatggt ggaagaatat
    21181 caggtcgatg gcgtagttga tgtgattttg caggcgtgcc atacctacgc ggtggaatcg
    21241 ctggcaatta aacgtcatgt gcgtcagcag cacaacattc cttatatcgc tattgaaaca
    21301 gactactcca cctcggatgt tgggcagctc agtacccgtg tcgcggcctt tattgagatg
    21361 ctgtaaggag tagcagtgac atattcgatt ggcattgatt ctggttctac cgccaccaaa
    21421 gggatcttac tcgcagacgg cgtgattacg cgccgtttcc tcgttccaac accctttcga
    21481 ccggcaacag caattactga agcctgggaa actctgcgcg aagggttaga gacaacgccg
    21541 tttctgacgc tcaccggcta cgggcggcaa ctggtggatt ttgccgataa acaagtgacg
    21601 gaaatctcct gtcacgggct gggcgcacgg tttcttgcgc cagcaacgcg cgcggtaatc
    21661 gacatcggtg gtcaggacag caaagtgatt cagcttgatg atgacggtaa cctgtgcgat
    21721 ttcctgatga atgacaaatg cgcggcgggc accgggcgtt tcctggaggt gatctcgcgc
    21781 acgcttggca ccagcgtcga gcaactcgac tgcattaccg aaaatgtcac gccgcacgcc
    21841 atcacgagta tgtgcacagt gtttgctgaa tcagaagtga tcagcctgcg ctcagcgggc
    21901 gtcgcgccag aagcgattct cgccggagtg attaacgcga tggcgcggcg gagtgccaat
    21961 ttcattgctc gtctctcctg tgaagcgccg attctgttta ctggtggcgt tagtcattgc
    22021 cagacgtttg cccggatgct ggaatctcac ctgcgaatgc cggtaaatac ccatcctgat
    22081 gcgcaatttg ctggcacaat tggcgcggcg gtaattggtc aacgagtgag gacacgccga
    22141 tgaaagagtt tttattttta tttcactcca cggtcggcgt catacaaacc cgcaaggcgt
    22201 tgcaggcagc gggcatgact tttcgcgtca gcgatattcc ccgtgattta cgcggcggct
    22261 gcgggttatg catttggctg acctgtcccc ccggcgagga aatacaatgg gtgatccctg
    22321 ggcaaaccga agcgatatat tgccagcagg gcagcgactg gcagtgcgtg gtgcattacg
    22381 atgcagaagc gtcaaccagg caataggatc tttaaatata ccaaccggat aattggctgc
    22441 gcataatcct gcatgaagga agttgtagta aatatctcgc ctgccgtgaa atatttccca
    22501 tccacaaaat gagcgatttt tgatagcacc ttcctattgc gcgtctaaaa tacaatatac
    22561 attgaattat acttaaaccc ataatcatgc atagagaaat aaaatgatta ccgggtgttg
    22621 taatgttaaa agataagttt ctgtgcaaag cttatctttc cccaccccct gattatttta
    22681 gatttagaag aatatttcac agttcaagga taaaaatatg ggctccctat ttaacatata
    22741 caaagatatt ttcccaacac tcggcatgta ttcgggacta aaagct
//
LOCUS       DAZFDC010000074        22532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFDC010000074 DAZFDC010000000
VERSION     DAZFDC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.074"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(102..278)
                     /locus_tag="ACHJ48_002923"
     CDS             complement(102..278)
                     /locus_tag="ACHJ48_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708438.1"
                     /translation="MEKDLKELREYLLLSPVYSEINDCLASLSMEDVTNEEFAERLGI
                     APDWMDAIDTSQLA"
     gene            581..1207
                     /locus_tag="ACHJ48_002924"
     CDS             581..1207
                     /locus_tag="ACHJ48_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4708439.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     RAKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKNELLIVKSE"
     gene            1558..1725
                     /locus_tag="ACHJ48_002925"
     CDS             1558..1725
                     /locus_tag="ACHJ48_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4708440.1"
                     /translation="MLHSAMHSLMNSQARDTRDRSQNDDNVLEISEPCIRSTFERLYQ
                     NMNEVFGLGDY"
     gene            complement(1805..3052)
                     /locus_tag="ACHJ48_002926"
     CDS             complement(1805..3052)
                     /locus_tag="ACHJ48_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO4708441.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3124..4038)
                     /locus_tag="ACHJ48_002927"
     CDS             complement(3124..4038)
                     /locus_tag="ACHJ48_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO4708442.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTEIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVDCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4254..5687
                     /gene="cscA"
                     /locus_tag="ACHJ48_002928"
     CDS             4254..5687
                     /gene="cscA"
                     /locus_tag="ACHJ48_002928"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO4708443.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(5695..6690)
                     /locus_tag="ACHJ48_002929"
     CDS             complement(5695..6690)
                     /locus_tag="ACHJ48_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4708444.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            6933..7151
                     /locus_tag="ACHJ48_002930"
                     /pseudo
     CDS             6933..7151
                     /locus_tag="ACHJ48_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7169..8497
                     /gene="dsdA"
                     /locus_tag="ACHJ48_002931"
     CDS             7169..8497
                     /gene="dsdA"
                     /locus_tag="ACHJ48_002931"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO4708445.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(8605..10143)
                     /gene="emrY"
                     /locus_tag="ACHJ48_002932"
     CDS             complement(8605..10143)
                     /gene="emrY"
                     /locus_tag="ACHJ48_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO4708446.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     SINLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVIFLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTETIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10143..11306)
                     /gene="emrK"
                     /locus_tag="ACHJ48_002933"
     CDS             complement(10143..11306)
                     /gene="emrK"
                     /locus_tag="ACHJ48_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO4708447.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            11722..12336
                     /gene="evgA"
                     /locus_tag="ACHJ48_002934"
     CDS             11722..12336
                     /gene="evgA"
                     /locus_tag="ACHJ48_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO4708448.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            12341..15934
                     /gene="evgS"
                     /locus_tag="ACHJ48_002935"
     CDS             12341..15934
                     /gene="evgS"
                     /locus_tag="ACHJ48_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO4708449.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEIIQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNVDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(15990..17131)
                     /gene="yfdE"
                     /locus_tag="ACHJ48_002936"
                     /pseudo
     CDS             complement(15990..17131)
                     /gene="yfdE"
                     /locus_tag="ACHJ48_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(17205..18149)
                     /gene="yfdV"
                     /locus_tag="ACHJ48_002937"
     CDS             complement(17205..18149)
                     /gene="yfdV"
                     /locus_tag="ACHJ48_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO4708450.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQTRAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(18219..19913)
                     /gene="oxc"
                     /locus_tag="ACHJ48_002938"
     CDS             complement(18219..19913)
                     /gene="oxc"
                     /locus_tag="ACHJ48_002938"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO4708451.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(19967..21217)
                     /gene="frc"
                     /locus_tag="ACHJ48_002939"
     CDS             complement(19967..21217)
                     /gene="frc"
                     /locus_tag="ACHJ48_002939"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO4708452.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(21730..22365)
                     /gene="yfdX"
                     /locus_tag="ACHJ48_002940"
     CDS             complement(21730..22365)
                     /gene="yfdX"
                     /locus_tag="ACHJ48_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO4708453.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         6526 a         5223 c         4863 g         5920 t
ORIGIN      
        1 cattttatca atctactgat tttgatggtg aaatttatta gaaggtatgc gtagtgctac
       61 gggagaaatg agaaacgcgc ccagcaatgg cgcatccttt cttaagcaag ttggctagta
      121 tctatagcat ccatccaatc aggagcaatt cccaaacgtt ccgcgaactc ttcattggtc
      181 acgtcttcca ttgagagact agccagacaa tcgttaattt cagaatagac gggggaaagt
      241 aataggtatt cccgcaactc tttcaggtct ttttccatct taacactcct tgttaataga
      301 ccgtcgaaca atggtacaga tttgtaccat tccaagtact ggcaagcaac cccaccagca
      361 caacaaatta tctcatacgg taaaaacggt gtcgggctgt ttctttcggc tagctcaata
      421 tatcacctta aacaccagcc gttatcattg tctcaattgg gtataccgta atgacaaggt
      481 gatacaaagt ctcaataggg ttgtatagga acattttgac tacacaaact gcattatgta
      541 tcatttgcac ttatattcac aacccttttg agacaatgca atgaacgtta gaatttactg
      601 ccgagcatca actgaggggc aacacgctga tcgagcactt accagcctac gtgaattttc
      661 aaaatcaaaa ggctggcaga ttgcaggaga gtatatagaa aatgctagcg gggcgaagct
      721 tgagcgcgta gagcttatgc ggctattgtc tgaagcccag tccggcgatc tgttgctagt
      781 ggaagctata gatcgtctga gtcgcctaga acatagtgca tgggtcgaac tgaaagatac
      841 gctcaaccga aaagggttaa tcatcgtatc aatggattta ccaacgagct ggcagatggt
      901 cgagatggca ggcaatgatc taaccagcgg catcctgaga gccgtaaatg ccatgttgat
      961 tgatatcctt gccacaatgg cacggcagga ttacgaaaca cgccgtaaac gccaacagca
     1021 aggcattgaa cgagctaaaa gcgaaggcat atacattggg agggcgaaaa atcaggaagc
     1081 ccgagagatc gttagagaga tgttggagca aggagtaaaa cctgaactca tcatgaaggc
     1141 agcaggaatc agccgagcga cttactaccg gataaaaaac gagctattga tcgtaaaatc
     1201 agaataaaat ttgaaaggtc gtcaggcttg tttcatcagg taaaaaccag catttctgat
     1261 cacaacgctt gggggcacct attggggcac tcaccaaaat cgaaagagaa ataaactaca
     1321 attcaaattg ttagctaatt tttcgattcc tgcagggaca ccagataccc ttcaaaccaa
     1381 atctaccttc atcccgtaaa cgatgggttt ggcagcacac ttgccctata tctactcatt
     1441 tttactgcaa caggttgaaa tctcagcact gtcagaaagc gctgatgact aaacagccca
     1501 gggccgggcg atgtaaccat cacacagaat cctgatagcg aaatacggcg tgactcgata
     1561 cttcactccg caatgcattc cttgatgaat tcgcaggccc gtgatacacg ggacaggtcg
     1621 cagaatgacg acaatgtcct ggaaatcagc gaaccgtgta tccggagtac atttgagcga
     1681 ctgtaccaga acatgaatga ggtgtttgga ttaggcgatt attagcaggg ctaagcattt
     1741 tagtattatt attttccggt tgaaggatat ggagatatcg acaacaaccg gaaaaagttt
     1801 acgtctatat tgctgaaggt acaggcgttt ccataactat ttgctcgcgt tttttactca
     1861 ggaagaaaat gccaaatagc aacatcaggc agacaatacc cgaaattgcg aagaaaactg
     1921 tctggtagcc tgcgtggtca aagagtatcc cagtcggcgt tgaaagcagc acaatcccaa
     1981 gcgaactggc aatttgaaaa ccaatcagaa agatcgtcga cgacaggcgc ttatcaaagt
     2041 ttgccacgct gtatttgaag acggatatga cacaaagtgg aacctcaatg gcatgtaaca
     2101 gcttcactaa tgaaataatc caggggttaa cgaacagcgc gcaggaaagg atacgcaacg
     2161 ccataatcac aacaccgata agtaatgcat tttttggccc tacccgattc acaaagaacg
     2221 gaataatcgc catgcacagc gcttcgagta ccacctggaa tgagttgaga taaccataca
     2281 ggcgcgttcc tacatcgtgt gattcgaata aacctgcata aaagacagga aaaagttgtt
     2341 gatcaaaaat gttatagaaa gaccacgtcc caacaataaa tatgacgaaa acccagaagt
     2401 ttcgatcctt gaaaactgcg ataaaatcct ctttttttac ccctcccgca tccgccgcta
     2461 cgcactggtg atccttatct ttaaaacaca tgttgatcat cataaataca gcgccaaata
     2521 gcgagaccaa ccagaagttg atatggggac tgatactaaa aaatatgccg gcaaagaacg
     2581 cgccaatagc atagccaaaa gatccccagg cgcgcgctgt tccatattcg aaatgaaaat
     2641 ttcgcgccat tttttcggtg aagctgtcaa gcaaaccgca tcccgccaga taccccaggc
     2701 caaaaaagag cgcccccaga attagaccta cagaaaaatt gctttgcagt aacggttcat
     2761 aaacgtaaat cataaacggt ccggtcaaga ccaggatgaa actcatacac cagatgagcg
     2821 gtttcttcag accgagttta tcctgaacga tgccgtagaa catcataaat agaatgctgg
     2881 taaactggtt gaccgaataa agtgtaccta attccgtccc tgtcaaccct agatgtcctt
     2941 tcagccaaat agcgtataac gaccaccaca gcgaccagga aataaaaaag agaaatgagt
     3001 aactggatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc ataattacct
     3061 gcctgtcgtt aaaaaattca cgtcctattt agagataaga gcgacttcgc cgtttacttc
     3121 tcactattcc agttcttgtc gacatggcag cgctgtcatt gcccctttcg ccgttactgc
     3181 aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctctct catctgtaga
     3241 taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc ccgcccccgt
     3301 gctatcgaca caatccacag acattccagc aaaatggtga acttgtcctc gataacagac
     3361 caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact cttttgccag
     3421 ggcgcatata tcccgatcgt tctgtgtttt tccactgata agtcgccatt cttcttccga
     3481 gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga gcaaatgctc
     3541 gtcttgccat agatcttcac gaatattggg atcgaagctg acaaaacctc cggcatgccg
     3601 gatctccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca acgcaattga
     3661 acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct ctaaaaaaag
     3721 atcggcactg gggcggacca taaacgtaaa tgaacgttcc ccttgatcgt tcagatcgac
     3781 aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat cgactccctc
     3841 agttagcagc gttctttgca ttaatgcacc aaaaggatca tcccccaccc gacctataaa
     3901 cccacttgtt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg cgccgccagg
     3961 acaaggcagt agccgcccgt ctgattctgg caagagatct acgaccgcat cccctaaaac
     4021 ccatactttg gctgacattt ttttccctta aattcatctg acttacgcat agtgataaac
     4081 ctctttttcg caaaatcgtc atggatttac taaaacatgc atattcgatc acaaaatgtc
     4141 atagttaacg ttaacatttg tgatattcat cgcatttatg aaagtaaggg actttatttt
     4201 tataaaagtt aacgttaaca attcaccaaa tttgcttaac caggatgata aaaatgacgc
     4261 aatctcgatt gcatgcggcg caaaacgcac tagcaaaact tcacgagcgc cgaggtaaca
     4321 ctttctatcc ccattttcac ctcgcgcctc ctgccgggtg gatgaacgat ccaaacggcc
     4381 tgatctggtt taacgatcgt tatcacgcgt tttatcaaca tcacccaatg agtgaacact
     4441 gggggccaat gcactgggga catgccacca gcgacgatat gatccactgg cagcatgagc
     4501 ctattgcgct agcgccagga gacgagaatg acaaagatgg gtgtttttca ggtagtgctg
     4561 tcgatgacaa tggtgtcctc tcacttatct acaccggaca cgtctggctc gatggtgcag
     4621 gtaatgacga tgcaattcgc gaagtacaat gtctggctac cagtcgggat ggtattcatt
     4681 tcgagaaaca gggtgtgatc ctcactccac cagaaggaat catgcacttc cgcgatccta
     4741 aagtgtggcg tgaagccgac acatggtgga tggtagtcgg ggcgaaagac ccaggcaaca
     4801 ctgggcagat cctgctttat cgcggcagtt cattacgtga atggactttc gatcgcgtac
     4861 tggcccacgc tgatgcgggt gaaagctata tgtgggaatg tccggacttt ttcagccttg
     4921 gcgatcagca ttatctgatg ttttccccgc agggaatgaa tgccgaggga tacagttacc
     4981 gaaatcgctt tcaaagtggc gtaatacccg gaatgtggtc gccaggacga ctttttgcac
     5041 aatccgggca ttttactgaa cttgataacg ggcatgactt ttatgcacca caaagctttg
     5101 tagcgaagga tggtcggcgt attgttatcg gctggatgga tatgtgggaa tcgccaatgc
     5161 cctcaaaacg tgaaggctgg gcaggctgca tgacgctggc gcgcgagcta tcagagagca
     5221 atggcaaact tctacaacgc ccggtacacg aagctgagtc gttacgccag cagcatcaat
     5281 ctatctctcc ccgcacaatc agcaataaat atgttttgca ggaaaacgcg caagcagttg
     5341 agattcagtt gcagtgggcg ctgaagaaca gtgatgccga acattacgga ttacagctcg
     5401 gcactggaat gcggctgtat attgataacc aatctgagcg acttgttttg tggcggtatt
     5461 acccacacga gaatttagac ggctaccgta gtattcccct cccgcagcgt gacacgctcg
     5521 ccctaaggat atttatcgat acatcatccg tggaagtatt tattaacgac ggggaagcgg
     5581 tgatgagtag tcgaatctat ccgcagccag aagaacggga actgtcgctt tatgcctccc
     5641 acggagtggc tgtggtgcaa catggagcac tctggcaact gggttaacat aatatcaggt
     5701 ggaacaacgg atcaacagcg ggcaagggat ccgcgtcaca cttccccctt cacgaccttc
     5761 aataatatgc aatgcagctt cccgcccgat aatgtcatgt ggaagctgaa ttgtggtcag
     5821 cggcggtaaa aacagatgcc cgacgccaac cagattatca aagcccatta cggcgacatc
     5881 ctgcgggata cgtaccccct tagccaggag aacctgataa gccacaaagg ctgcgcgatc
     5941 gttaccacat atcagaacat caaaatctgg tttgcccgat ttgaagtggt cattgagtaa
     6001 acttgcgaga tcggtgtagt gatcatcacc tgttgccatg tgaaattgtt tcacctcagc
     6061 cagatctcgt ccagcatcac gccaggcctg ctcaaatccc tgccgacgat accctgttgc
     6121 caacgcactt tccggtagcc agaagcataa cggttgacga tagcccgccg cgagcaaatg
     6181 ctgtgttgat tcatattgtg cagtgtaatc atcagggata taactgggta acgctgggtc
     6241 atccgccaca cagttcgcca atacaatatt ttcaccatac agagactcag gtagcgtgat
     6301 atgtcgcagc cccattgtag tatagataat gccatccgga cggtgggcaa gcagctgacg
     6361 tgccgcgcgg gcagcgtcat cttcagaaaa aatattgatt aaaaaactat tccagccgaa
     6421 ctcgctggcg gtttgctcaa tggcaagcag aatatcaaca gagaaaggag tggtagcagt
     6481 gtcctgcgcc agcacggcga gagtcgacgg cttacgtcct tgagcgcgca tcttacgggc
     6541 ggaaagatca ggaacataat tcagggtctg gattgcctgc aatacgcggt cacgcgttgc
     6601 aggacgcaca gattctgcat tatgcatcac ccgggagact gtcatcatcg acactcccgc
     6661 caggcgtgcg acatccttta atgaagccat acccaagccg tttgccgtaa aacgggcact
     6721 gtagcagaaa cagacgtcac tggcgagatc caacgaaatt ctcgacgccc tatcacccga
     6781 cacggcaata caataaaaaa taacaataat tgccggataa ttgtacccaa ttccgcctct
     6841 gttctcgcca acgagtgacc atataggcaa gtaagaaaaa catgccaact ggcatcgcca
     6901 acctggagaa gaaaatataa atatggatca ttgtgctgcc gctgtatccc gacatcagcc
     6961 cggaaattat tgcgattgct atcggttcag gcgcgattgg ctgcacgatc gttacggact
     7021 cgcttttctg gctggtgaag caatattgcg gcgctacgct caatgaaaca tttaaatact
     7081 atacgacagc gacatttatc gcttcagtcg tcgctctggc gggcacattc ctgctgtcat
     7141 ttatcatcta agcgcaaaga gacgtactat ggaaaacgct aaaatgaact cgctcatcgc
     7201 ccagtatccg ttggtaaagg atctggttgc tcttaaagaa accacctggt ttaatcctgg
     7261 cacgacctca ttggctgaag gtttacctta tgttggcctg accgaacagg atgttcagga
     7321 cgcccatgcg cgcttatccc gttttgcacc ctatctggca aaagcatttc ctgaaactgc
     7381 tgccactggg gggattattg aatcagaact ggttgccatt ccggctatgc aaaaacggct
     7441 ggaaaaggaa tatcaacaac cgatcagcgg gcaactgtta ctgaaaaaag atagccattt
     7501 gcccatttcc ggctccataa aagcacgcgg cgggatttat gaagtcctgg cacatgcaga
     7561 aaaactggct ctggaagcgg ggttgctgac gcttgaagat gactacagca aactgctttc
     7621 tccggagttt aaacagttct ttagccagta cagcattgct gtgggctcaa ccggaaatct
     7681 ggggttatca atcggcatta tgagcgcccg cattggcttt aaggtgacag tgcatatgtc
     7741 tgctgatgcc cgggcatgga aaaaagcgaa actgcgcagc catggcgtta cggtcgtgga
     7801 atatgagcaa gattatggtg ttgccgtcga ggaaggacgt aaagcagcgc agtctgaccc
     7861 gaactgtttc tttattgatg acgagaattc ccgcacgttg ttccttgggt attccgtcgc
     7921 aggccagcgt cttaaagcgc aatttgccga gcaaggtcgt attgtcgatg ctgataaccc
     7981 tctgtttgtc tatctgccgt gtggtgttgg cggtggtcct ggtggcgtcg cattcggact
     8041 taagctggcg tttggcgatc atgttcactg cttttttgcc gaaccaacgc actccccttg
     8101 tatgttgtta ggcgtccata caggattaca cgatcagatt tctgttcagg atattggtat
     8161 cgacaacctt accgcagcgg atggccttgc agttggtcgc gcatcaggct ttgtcgggcg
     8221 ggcaatggag cgtctgctgg atggcttcta tacccttagc gatcaaacca tgtatgacat
     8281 gcttggctgg ctggcgcagg aagaaggtat tcgtcttgaa ccttcggcac tggcgggtat
     8341 ggccggacct cagcgcgtgt gtgcatcagt aagttaccaa cagatgcacg gtttcagcgc
     8401 agaacaactg cgtaatgcca ctcatctggt gtgggcgacg ggaggtggaa tggtgccgga
     8461 agaagagatg gagcaatatc tggcaaaagg ccgttaataa cgtttcaacg cagcatcgca
     8521 atcctttccc tgggtgagcg atgctgccga tggcgcagac ttaagatccc cagtcttacc
     8581 cgctataacc ccctgctttt accatcaccc aacgcctttc gctgtaaacg gcggtttcgc
     8641 aaaccaaacc aaaaccgtaa gtaagataaa agcaatagcc gccatacgga aaatctcatt
     8701 tgcagaaata gaaagtgact gctgggttat ttcattatta atttcattaa gaactcctga
     8761 aagcgaacca tagtatttat ccataatttg tgacgatgaa ttaaacacgg ggttaaattg
     8821 atcgatggtt tctgtcaact gactatggtg taacgattcg cgtcgtcccc acagcgtcat
     8881 tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa aaattactca tactcgaggc
     8941 attggcaaat ttattatctg gcaagcctga aaacgaaatc gttgttaagg gtaaaaagaa
     9001 acaggcaacg gcgaatccct gaaaaaactg cggcaaaatg atacctgtaa aatcaatcgt
     9061 tggcataaat gtcacagaac gccagtaata gcaaaccgca tacatcaaaa aactgaatgt
     9121 caccaacacc cgcatgtcta ttttgttgcc ataacgtcct atcaaaggtg aaattaatag
     9181 tggcatgatg ccgatgggcg cataagcaag tccggcccat atcgcattat accccatcgt
     9241 ttcctggagt aactgcggca taaggacgat cgctccagag taaaataaat acgcgcatgt
     9301 gatactcaca ataccaatgg tgaagttacg ggacttaaac aaactgagat caagaatcgg
     9361 gttctctgag gtcgactccc aaatgactaa agagatcaga aaaataactg atactactgt
     9421 taatattatt attgtactcg agttgaacca atccagatcg cgccctttat caagcataat
     9481 ttgcaagcca ccaacaccga gcactaacag ggtcagtcct ggtagattca ttttgaccgg
     9541 tgaagtctca gtttctcttc ctttaagtaa ggttaagcat aatgtcagga cgataatccc
     9601 catagggaca ttgattaaaa atatccaacc ccagctaaag ttatcacaaa tataaccgcc
     9661 caatatcggc ccacatatcg gagcgataat cacggtcatt gaccataatg ccagagcaaa
     9721 tgttcttttt tctggtggat aattccttaa taataaactc tgtgacagtg gaattaacgg
     9781 ccctgccatt aacccctgaa cgactctaaa aaatatcagc acatcaagat tgatcgataa
     9841 actacacatt aaggaagaca gcgaaaaaaa agtgactgaa agtaaaaata accttaattc
     9901 gcctattctt tgtgccagcc tgccggtaac agggatcgca atggcatttg ctacgccaaa
     9961 cgaggtgata acccaggtgc cttcgtctgt tgatgctccc agaaagccag atattgtcgg
    10021 tattgcgacg ttagaaatag tggaatccaa catttgcata aacgtcgcta atgacaatgc
    10081 aatagtgacg caccataacg tcccaccggt taatggtgcc ggagttgatt tagtgattgc
    10141 cattaaagtt gtccattatg cgaaataatg ttgctaattt ctttttctat cggactggta
    10201 tcgataacta aagccttact ggtataagcc ggcatggacg tcacggttga agccagctca
    10261 ggcatctcag caatgtcttc gttcttcgta tcaatagttg cagtcatcga taaaccaatg
    10321 cgcaaggggt gttccatgag ttcttttgga tcaagagaaa cttcaaccgg tacacgctga
    10381 acgattttga tccagttccc tgtcgcattt tgtgcaggta ataaggagaa cgcattgccg
    10441 gttcccatat tgatccctgt cacccggcca tgaaacacaa cattctcacc ataaagatcg
    10501 ctgataatat tgaccgattg accaatccgt acatccgtga gttgtgtttc tttaaagtta
    10561 gcattaaccc acatttgacg tgccggtacg acagccatta acgattgtcc gggactcact
    10621 gtttcgccga cctgaacact tctctgggca atatagccgg taaccggact cttaatatcc
    10681 gtacgtttaa gcgccaacca ggcttctttg gttgcatccg cagcttcaac gacttgtggc
    10741 tgacggttta atggtgtgtt cattactaaa gctttattcg ctttataagc ctggatagcg
    10801 gcattcaatg ccgctttgct acttattaac gtatctttgg tatgctccag cgtttctttt
    10861 gaaataaccc cctgcttcgc taaaggcaca cgacggttat aatcttctaa agattgttga
    10921 tactgaatac gtgctgaagc gacttcggca ctgtattgtt tatcctgtaa gtacagttta
    10981 ttcgtttgcc gaacaatatt tgccagatta tttttagctt tattgagtgc gatagtggca
    11041 tcagttttat ccagtgaaac taaaatgtca ccctgtcgaa cgtagttcgt atctttatga
    11101 ttaacgacag tgacactacc tgagacttgt gcagaaattg gatctgcatt ccccgtgaca
    11161 taggcgtcat ctgtactaat catgtcttct aattccattg accaataggc ataagcacct
    11221 gaaaacgcaa taaataaaac tatcgccaat aaagaaaagt attttcttct gttagaatgt
    11281 tttttatttg aattaatctg ttccactatt atctctcatt tcgcatagat gaaatttatg
    11341 aattgaatga aaagagtatt cattcttcat atcaaacaca gtgcatgtgt tgcacattat
    11401 ccctggaacg ttggaggcct aatttaaatg aggtgcaaaa aaaacaacag aggactaaac
    11461 cgtggctttt gcaatacaat tcttacgcct gtaggattag tgagaagact tatagtgcta
    11521 acttgaaact ataaatcatc ggtataatcc ctgattttat tgttgacatt tcatatatgc
    11581 cgactattta tatggtatac ttgtcgaatt atcttaaagg aagctcaaat tttcttattt
    11641 ttattgagaa aatgagatga tgccttatgt ctgtattact acagggagaa gggagatgct
    11701 tcattgcaaa gggaataatc tatgaacgca ataattattg atgaccatcc tcttgctatc
    11761 gcagcaattc gtaatttatt gatcaaaaac gatattgaaa tcttagcaga gttgactgaa
    11821 ggcggaagtg ccgttcagcg ggtggaaaca cttaagcctg atatcgtcat cattgatgtc
    11881 gatatccccg gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg ccaatatagc
    11941 ggaattatta ttatcgtctc cgctaaaaat gaccattttt acgggaaaca ttgtgctgat
    12001 gctggcgcta atggtttcgt gagtaaaaaa gaaggcatga acaatatcat tgcggctatt
    12061 gaagctgcaa aaaatggcta ctgctatttc cccttctctc tcaaccggtt tgttggaagt
    12121 ttaacgtccg accagcaaaa actcgactcc ttatcgaaac aagaaattag tgtcatgcgg
    12181 tatattcttg atggcaagga taataatgac attgctgaaa aaatgttcat cagcaacaaa
    12241 actgtcagca cttataaaag tcgcctgatg gaaaaattag aatgtaaatc actgatggat
    12301 ctttacacat tcgcacaacg taacaaaatc ggctaaccac atgaagtttt taccctatat
    12361 ttttcttctc tgttgtggtc tttggtcgac cataagtttc gcagacgaag attacatcga
    12421 atatcgtggc atcagtagta acaaccgtgt cacacttgat ccactacgtc tgagcaacaa
    12481 ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt gcagtacaca agtcccaaac
    12541 ggctacgttg ttgcataccg attcgcagca acgggttcgt ggtattaatg ctgattattt
    12601 aaatctttta aaaagagcgt taaatatcaa attaacactc cgggaatacg cagatcatca
    12661 aaaagcaatg gacgcgctgg aagaaggtga agtcgatata gtgttatcac atttagttgc
    12721 ttcgccgcct cttaatgatg acattgctgc aaccaaacca ctgataatta cctttccggc
    12781 gctggtcacc acccttcacg attcaatgcg accgcttacc tcatcaaaac cagtaaatat
    12841 tgctcgagta gcaaattatc cccctgacga ggtaattcat caatcatttc caaaagcaac
    12901 aattatctct tttacaaatt tatatcaggc attagcatcc gtctcagccg gacagaatga
    12961 ttactttatt ggtagtaaca tcattaccag cagtatgatt tcccgctatt tcactcactc
    13021 cttaaatgta gtgaaatatt ataactcacc gcgtcaatat aacttcttct taaccagaaa
    13081 agaatctgtc attcttaatg aagtactcaa tcgatttgtt gatgctttaa caaatgaagt
    13141 tcgctatgaa gtatcacaaa attggcttga tacaggaaac ctggcctttc tgaacaagcc
    13201 attagaactc actgaacatg aaaaacagtg gattaagcag catcccgatt taaaggtgct
    13261 ggaaaatcct tactcgcccc cctattctat gacggatgaa aatggctcgg ttcggggcgt
    13321 tatgggggac attcttaata ttattacctt gcaaacaggt ttaaattttt ctccgatcac
    13381 cgtttcacac aatatccatg ctggaacaca gctgaatccc ggcggatggg atatattacc
    13441 cggtgctatt tatagtgaag atagagaaaa taatgtttta tttgctgaag ccttcataac
    13501 aacgccttac gtttttgtca tgcaaaaagc gcctgacagt gaacaaacat taaaaaaagg
    13561 aatgaaagtt gccattccat attattatga gcttcattcg caattaaaag agatgtatcc
    13621 ggaggtagag tggataaaag tcgataacgc cagcgctgca tttcacaagg tcaaggaagg
    13681 cgaacttgat gctctggtcg cgacacagtt aaattcacgt tacatgatcg accattacta
    13741 tcctaatgaa ctgtatcatt ttcttattcc cggcgttccg aatgcatcgc tttcgttcgc
    13801 ttttcctcgc ggagaaccgg aacttaagga tattattaat aaagcactga atgcaatccc
    13861 cccaagcgaa gttctgcgcc tgaccgaaaa atggattaaa atgcccaatg tgaccattga
    13921 cacatgggac ctctatagcg agcaatttta tattgttacg acattatccg ttttattagt
    13981 tggcagtagc cttttatggg gattctacct gttacgctca gttcgtcgtc gtaaagtcat
    14041 tcagggtgat ttagaaaacc aaatatcatt ccggaaagcg ctctcggact ccttaccgaa
    14101 tccaacttat gttgtaaact ggcaaggtaa tgtcattagt cacaatagtg catttgaaca
    14161 ttatttcact gctgattact acaaaaatgc aatgttgcca ttagaaaaca gtgaatcacc
    14221 ctttaaagat gttttttcta atacgcatga agtcacagca gagacgaaag aaaaccgaac
    14281 aatatacaca caggtatttg aaattgataa tggcatcgag aaaagatgca ttaatcactg
    14341 gcatacatta tgtaatctgc cagccagcga acatgctgtt tatatttgtg gttggcaaga
    14401 tattaccgag acgcgtgatt taattcatgc actcgaagta gagagaaata aagcgatcaa
    14461 tgcaactgtc gcaaaaagtc agtttctggc aacaatgagt cacgaaataa gaacaccaat
    14521 aagctccatt atgggcttcc tggaacttct gtcgggttct ggtcttagca aggagcaacg
    14581 ggtggaggcg atttcactag cctacgccac cggacaatca ctcctcggct taattggtga
    14641 aatccttgat gtcgacaaaa ttgaatcggg taactatcaa cttcaaccac aatgggtcga
    14701 tatccctact ttagtccaga acacttgtca ctctttcggt gcgattgctg caagcaaatc
    14761 gatcgcatta agttgcagca gtacatttcc tgatcattac ctggttaaga tcgaccctca
    14821 ggcgtttaag caggtcttat caaatttact gagtaatgct ctcaaattta ccaccgaggg
    14881 ggcagtaaaa attacgacct ccctggttca cattgatgac aaccacgctg taatcaaaat
    14941 gacgattatg gattctggaa gtggattatc acaggaagaa caacaacaac tgtttaaacg
    15001 ttatagccaa acaagtgcag gtcgtcagca aacaggttct ggtttaggct taatgatctg
    15061 caaagaatta attaaaaaca tgcagggtga tttgtcatta gaaagtcatc caggcatagg
    15121 aacaacattt acgatcacaa tcccggtaga aattatccaa caagtggcgg ctgtcgaggc
    15181 aaaagcagaa caacccatca cactacctga aaagttgagc atattaatcg cggatgatca
    15241 tccgaccaac aggctattac tcaaacgcca gctaaatcta ttaggatatg atgttgatga
    15301 agccactgat ggtgtgcaag cgctacacaa agtcagtatg caacattatg atctgcttat
    15361 tactgacgtt aatatgccga atgtggatgg ttttgagttg actcgcaaac tccgtgagca
    15421 aaattcgtcc ttacccatct gggggcttac agccaacgca caggctaacg aacgtgaaaa
    15481 agggttaaat tgcggcatga acttatgttt gttcaaaccg ttgaccctgg atgtactgaa
    15541 aacacattta agtcagttac accaggttgc gcatattgta cctcagtatc gccaccttga
    15601 tatcgaggcc ctgaaaaata atacggcgaa cgatctacaa ctgatgcagg agattctcat
    15661 gactttccag catgaaacac ataaagattt acccgctgcg tttcatgcac tagaagctgg
    15721 cgataataga actttccatc agtgtattca tcgcatccac ggtgcggcta acatcctgaa
    15781 tttgcaaaag ttgattaata ttagccatca gttagaaata acacctgttt cagatgacag
    15841 taagcctgaa attcttcagt tgctaaactc tgtaaaagaa cacattgcag agctagacca
    15901 ggaaattgct gttttctgtc aacaaaataa ctaaatagtg gctcccacaa tgttcaaatg
    15961 tgggagctat ttaccagcac atcttacatt tatgatgaga actcctggcg aatttgctcc
    16021 ccatgctggt cgagcgttgc cgctcccggc ataacatgcg ggtccgcgca gccactgatt
    16081 tttatcggat tacccggcat cattattccc ccggcttcaa tcaacatatt tctcgcctga
    16141 gtttgtggca aattaatggc ctcagccaca cttaataacg gcgcgacggg cacaccaact
    16201 tcatgtattc tggctaacca aacttcagct gcctgtgttt ttaacgtccg ctcaatatat
    16261 tgtttaagaa tagtctggtt ttgtacgcgt aaaatattgc tgctaaatct gggatcatta
    16321 accagttccg taagctccag tgcctggcat aacgcagaga aaagcttgtc attaccacaa
    16381 caaatcgtaa tcggcttatc ctgagtatcg aaaacatcaa aaggtgccat gtaggggtgg
    16441 cgatttccca ggcgttgtgg tgacttccct gtcgcaatat atgccatcag accatgctcc
    16501 agaaaactca gcgtggcatc aaacatcgct atatcgacat gcgcccctct ttggctcttt
    16561 tcgcggccat aaagtgcact cactattccg ctgaataaat aaacaccgcc gcatagatcc
    16621 gcaagagagg taccaacacg cactggcgga gcatcagggt accctgtttc catcattatc
    16681 ccgctcattg cctgaatgat ggtatcgtag gcaggagcat cttttagcgg accggtatgt
    16741 ccgaaacctg acgatgaagc atatatgagg cgcgggttga tttcttgtaa cctttcccat
    16801 gaaagcccca gtttttccat tgtacctggg cgaaaattct cagctaatac atcagcttgt
    16861 ttaagcatat ttataaatat acttttatcg tgatcattct ttaaatcaag aaccacactc
    16921 tctttaccat gattaataaa actgtaatag agtgtccatc cacataggga ccaaatgtgc
    16981 gggtatcatc accatgaccc ggcggctcaa ctttaattac ccttgcgccc atattacaaa
    17041 gaagttgagt tccgaaaggt ccattaagga catgtgtcat atcgataact aataagcctt
    17101 caaacggccc tttgctttca ttatttgtca tttgcttatg ccttgaaaaa agccgaaggt
    17161 gaaaagacac ttcggcattt atgtaaaatc aataacatga ttaattatga aaccagtcga
    17221 ctgacataaa tccacaaggg agccgtgacg acaaaaccca gtacgctcac agccaatgac
    17281 gctgtaccag tgcgggtgta gacattaaac cggctggcaa taatgattcc ggagaatgcc
    17341 ggcggtaatg cgcctgccag taccatcatc tgcagatgtt cgctgttcaa atgacatgcc
    17401 ataccaacaa gaagcagcgc cagtggcatc agaatcagct tcaggaaggt gttataagca
    17461 atttcggcac tgaactcgaa tttatgtgca gccagagtca accccgcagc gaataccgct
    17521 acccctgagt tagccttcgc aatcagatta aaggttgggt cccatgctgc cggaattttt
    17581 accccaacca acaccaggat cgttgccaga acaggtgccc atacaactgg ctcctttgcc
    17641 gcagaaatta atgcgctcag attactattc ttcttaccat ccgctcctga ggaaggattc
    17701 agcaaataca gaccaatagg aatagtaatt gcgttaacaa taatagaaat aattgctacc
    17761 actaaacctg ttgatacgga atcaccataa ataggatcga gaactgcaaa ccccaggaat
    17821 ccaatggtag gtgaacctgc aattaatgca catacagctg cttctgcatg ggtacgttta
    17881 aaaaatttgt agcaaccaaa ccaggagaaa aagaaacatc cgacaataac cacgagtgat
    17941 acaagggtca gacgagtgtc cgcaaaaatc atttccctgt ttgcccgggt aatagaaaca
    18001 aatagggctg caggaagcgc gtagtttaat accagtttat taaatgcccg ggtttgatct
    18061 tctgaaaaag tttctcgtct gccgctaaaa taacccaaca gcatgattac gataataggc
    18121 aataaatcgc caataaaaaa tgttagcata atagtatttc cgataagggt cccctcacca
    18181 tcgcataatg agttggcaag aggagacgat gtcagtattt aattaccagc gacttgtttt
    18241 gggttaagtt tggtaatatg gccactttca gttcctgctg cagggtcgat gaccacatta
    18301 ataatggtcg gtttgcgcga ctgaataccg gtggttaaag catgacgaag ttcatctgtc
    18361 gtggtgacgt tatagccaac gccacgaaac gcatccatta atttgtcata ccttgcatgg
    18421 tgcaacagat ctgttggtga tggtgcacca gcgccactga gatcaacacc gtctcctctg
    18481 tagatgccgc cattattaaa aataacgatc gttaccggca ggttatatcg acaaatcgtt
    18541 tcaatttcca tcccactgaa accaaaagca ctatcacctt caatggcgac aaccggagaa
    18601 ccagaggtca cgctagcacc gatggcatag cccataccga tgcccatgac accccaggta
    18661 ccacaatcca gacgacgacg tggtttatac atatcaataa tatttcgtgc attatccagg
    18721 gtatttgcac cttcattaac taaataaata tcctggttct cacgcaatac atcgcgcaca
    18781 gcacttaatg cattaaagta atttaatggt tgggtatctg tacttaattt ttcatgcatt
    18841 ttttgtgcat tttgctgctt gtggatattt aaaatatcgc gccataccag tggagtcgta
    18901 aatgtgtttt gtttcagctc tgccagcata ccttgcatac tggatgcaat atcgccaacg
    18961 actggcacag caatggggcg gttgctgtca atttcctgcg gttcaatatc cagttgaata
    19021 aactgtgtat ctgccgccca tcctttttta ccgtgtgcca gtaaccaatt cagtcgtgca
    19081 ccaacaagca tgacaacgtc agcatttgcc agggcaaacg aacgcgcagc tgccgcagaa
    19141 agtggatgcg tatcttcgag gatccctttc gccatagaca ttggcaggaa tggaatctgg
    19201 gcactttcaa taaattcacg aagctgttcg tcagcttgtg aatacgccgc gcctttgcca
    19261 aggataatta atggccgttc agcttttgct aaaagcgaaa ttgcgctagt gactgacttc
    19321 gggcatggca ataatgctgg cgacggattt tcaactttta caatcgtggt taacgcttcg
    19381 tctttttcca tcgtcgcggc caggacattt gctggcaaat caagataaac tccgccaggg
    19441 cgacccgata ctgagacccg gatagcgcgt gccaatgcaa tgccaagatc ctgcggctga
    19501 ttaacgcgaa atgctgcttt ggcatacggt tttgccgcat tcatttggtc cagctcttca
    19561 taatcacctt gctgtaggtc gacgatcgcg cggtcgctgg agccgctaat catgatcatc
    19621 ggaaaaccat ttaccgttgc gttggccaat gcggtcaaac cattgaggaa tcctggcgca
    19681 gacactgtca ggcagatccc tggtttttgg gtaagaaaac cgcttgccgc agcggcatag
    19741 cctgccgact gctcatgacg aaaaccaata taacgaatgc cttccgcctg ggcatggcgc
    19801 gccatatccg tcacaggaat acctacaaca ccataaatag tgtcaatatt attctgtttt
    19861 aatgcttcaa cgatgatatg cataccatct gtcatttgaa gttgatctga catgattaat
    19921 aacctcgaaa tttttataaa ttgggagcca gaagcccccg ttaatatcag atggcgtggt
    19981 tttgcttcat tgcagcaatt tcatcgtcgc tataacccag ctcctgcaat accgcagcgg
    20041 tatgttcacc taatagaggc gcagctttaa tatccggcgt aaaggcagag aatttcattg
    20101 gacaaccaac tgtcagatat tttccacgca atggttgttc cacttcgaca acactgccgc
    20161 tttggcgtaa agagggatca agtgaaattt ctttcatact taaaaccggt gcacaaggaa
    20221 tatcaaactg agtcaaataa gccactgctt catgtttatc aatagtgaca gtgtattttt
    20281 cgatttcagc aaaaatatcg aaaatatgtg gctgtcgggc atgggctgta ctgtatgccg
    20341 gatcggtaat ccaatctggt ttgccgatgg ctttacaggt gttttcccag ttttgctcct
    20401 gaatagtgaa ataaatatag gcgttaggat cagtttccca gcctttacat ttcaggatcc
    20461 agccaggctg accgccacca cccgcattac ctccgcgggg aactgcatca ccaaatgtgc
    20521 cattcggata ctgcgggtat tcttccagat aacccaattt atcgagacgc tgctggtcgc
    20581 gtaatttcac gcggcaaagg ttcaatacgg catcctgcat tgacatggtg actcgttgcc
    20641 cacgccccgt tttttcgcga tgcagcaaag cagcaagtaa accgatcagt aaatgcattc
    20701 cggtgttgct gtcacccaac gctgcagcgc ttaccagcgg cggaccgtcc caaaaacccg
    20761 tagtggatgc cgcgccaccc gctgcctgag caacgttttc ataggctttt acattcacat
    20821 aaggcgaaca ctcatcaaac cctttgatcg aaccaaaaat cagacgtgga ttgatttctt
    20881 gaatatgctc ccaggtgaag cccatgtgat caatggcccc tggatgaaag ttctcgacta
    20941 agatatcagc ttcgcggatc agcttttcca ttacctcttt gccttccgct gttttggtat
    21001 ttaactcaat agaacgtttg ttactgttaa gcatggtgaa gtaaagcgca tcgatatcag
    21061 gaatatctcg cagctggtga cgcgttacgt caccaacgcc ggggcgttca attttaatga
    21121 cgtcagcgcc aaaccaggcc agcatttgag tacaagatgg gccagattgc acaccggtga
    21181 aatcgagaac tttaattcct tgaagtggag ttgacatagc aatacctatt agttaattaa
    21241 atatgtgtta atttagcgtt tatttaaggc ataacgccct ctataaataa tccgcaaaat
    21301 atcgtcatag cggcactcga tatttttaaa ttcctgtatc aagtgctgaa aatcatttca
    21361 tcgctacttc caattccttt ctgcatggta agtagtgagg acgaaaagaa gaatatgata
    21421 aatattggtt cgtggtaatc agagagagtc gcgacatttg tgatgttatt cttacaactg
    21481 taagaagcgc cggtgattta caagtaaggg tatgattacg ctttttagag cgtaccataa
    21541 aatataacat attaaatgat ttgtgatgac acgggattat tgcggtttac tttgaggaat
    21601 ataaaatgcg agtttataga acagcgagcg ccaaataatt ttatgaccac aacctgtcgt
    21661 attgtggtca taaaaaattg tcggctggag ggagttaaat cctttacctc aatatactgc
    21721 ctgtcagcat tagttcacaa acagtgcttc gctatcgacg atgattccat cttccgcacc
    21781 tttaagcgcc aggtttgcct cgtagtattg ctttttatca agcagtttct gcgcatcagc
    21841 aagtgcatta cgagtctgtt taagcggcat cagatattgg ttttccatta caccaacacc
    21901 ggccaggcga agctcttcca ttgcgccctt tttatcccct ttcgccattt tttcattggc
    21961 aatttttatc gcggcttctt tttcgggtgt cgctacataa ctctcagaaa ttccgacaga
    22021 tgcattaatg acaatatatt ggtcatcatt gacattggtc tttttacctg gcttagcgaa
    22081 ctttgcccat tcggtactgt catctgacaa caaagctgaa gcttcattgg tcagttcttt
    22141 tgccttttcc ggatcgccat gaaacagagc caggcgcgct acctgcacgt cacgcatcgc
    22201 atataaacct tgttcagaga tacgctctgc tgcagctgcc gttttctggg tttgctgaac
    22261 ttgctgagtt tgctgttgtg ccgctaccgg agcattatca gcagcccata cagttgaaga
    22321 tgccagaata gctgtgacca tcgtagccat aattaaacgt ttcatactta cctcattctt
    22381 taacagtttt agaaaacgcc gccatcatat tgggtgagca tgaaatgaga aatcagagag
    22441 caacggtcat tttgaaaggg ttatcttaca gttgtagtta ttatcgcaag aagtatttta
    22501 gtcttacaat tgtaggaata tgcttcctgt ca
//
LOCUS       DAZFDC010000075        22352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFDC010000075 DAZFDC010000000
VERSION     DAZFDC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.075"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            201..869
                     /locus_tag="ACHJ48_002941"
     CDS             201..869
                     /locus_tag="ACHJ48_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4708454.1"
                     /translation="MFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNKIDLLE
                     KQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKYFILNN
                     GSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEFARWQQ
                     LLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSAENRQK
                     RSKK"
     gene            913..5679
                     /gene="ehaG"
                     /locus_tag="ACHJ48_002942"
     CDS             913..5679
                     /gene="ehaG"
                     /locus_tag="ACHJ48_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO4708455.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIA
                     IGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALG
                     ADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGA
                     AVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASV
                     ITDVADGTISASSKDAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNIT
                     NLTDSVGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKT
                     TNDAVATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTET
                     TSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGE
                     DALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGG
                     NASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHG
                     TNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYT
                     IANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAIS
                     AASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALD
                     DNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQ
                     NTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGD
                     SSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRDTSITENGVVIGYDTTDGELLGAL
                     SIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGT
                     DSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRG
                     AQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQ
                     NTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGSTKYFKTNTDGVDASAQGKDSVAIG
                     SGSIAAADNSVALGTGSVATEENTISVGSSTNQRRITNVAAGKNATDAVNVAQLKSSE
                     AGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDVVNYAQLKQSVQETKQ
                     YTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVA
                     LGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            6047..7702
                     /gene="lldP"
                     /locus_tag="ACHJ48_002943"
     CDS             6047..7702
                     /gene="lldP"
                     /locus_tag="ACHJ48_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO4708456.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7702..8478
                     /gene="lldR"
                     /locus_tag="ACHJ48_002944"
     CDS             7702..8478
                     /gene="lldR"
                     /locus_tag="ACHJ48_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO4708457.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTIRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8475..9665
                     /gene="lldD"
                     /locus_tag="ACHJ48_002945"
     CDS             8475..9665
                     /gene="lldD"
                     /locus_tag="ACHJ48_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO4708458.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9851..10324
                     /gene="trmL"
                     /locus_tag="ACHJ48_002946"
     CDS             9851..10324
                     /gene="trmL"
                     /locus_tag="ACHJ48_002946"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO4708459.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10377..11198)
                     /gene="cysE"
                     /locus_tag="ACHJ48_002947"
     CDS             complement(10377..11198)
                     /gene="cysE"
                     /locus_tag="ACHJ48_002947"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO4708460.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11278..12297)
                     /gene="gpsA"
                     /locus_tag="ACHJ48_002948"
     CDS             complement(11278..12297)
                     /gene="gpsA"
                     /locus_tag="ACHJ48_002948"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO4708461.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12297..12764)
                     /gene="secB"
                     /locus_tag="ACHJ48_002949"
     CDS             complement(12297..12764)
                     /gene="secB"
                     /locus_tag="ACHJ48_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO4708462.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12827..13078)
                     /gene="grxC"
                     /locus_tag="ACHJ48_002950"
     CDS             complement(12827..13078)
                     /gene="grxC"
                     /locus_tag="ACHJ48_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO4708463.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13220..13651)
                     /gene="yibN"
                     /locus_tag="ACHJ48_002951"
     CDS             complement(13220..13651)
                     /gene="yibN"
                     /locus_tag="ACHJ48_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO4708464.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13896..15440
                     /gene="gpmM"
                     /locus_tag="ACHJ48_002952"
                     /gene_synonym="pgmI"
     CDS             13896..15440
                     /gene="gpmM"
                     /locus_tag="ACHJ48_002952"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO4708465.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15474..16733
                     /gene="envC"
                     /locus_tag="ACHJ48_002953"
     CDS             15474..16733
                     /gene="envC"
                     /locus_tag="ACHJ48_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO4708466.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16737..17696
                     /gene="yibQ"
                     /locus_tag="ACHJ48_002954"
     CDS             16737..17696
                     /gene="yibQ"
                     /locus_tag="ACHJ48_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO4708467.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17683..18718)
                     /gene="waaH"
                     /locus_tag="ACHJ48_002955"
                     /pseudo
     CDS             complement(17683..18718)
                     /gene="waaH"
                     /locus_tag="ACHJ48_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(18957..19982)
                     /gene="tdh"
                     /locus_tag="ACHJ48_002956"
     CDS             complement(18957..19982)
                     /gene="tdh"
                     /locus_tag="ACHJ48_002956"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO4708468.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMCSGQSG
                     KVILSWD"
     gene            complement(19992..21188)
                     /gene="kbl"
                     /locus_tag="ACHJ48_002957"
     CDS             complement(19992..21188)
                     /gene="kbl"
                     /locus_tag="ACHJ48_002957"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO4708469.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21463..22320)
                     /gene="yibB"
                     /locus_tag="ACHJ48_002958"
     CDS             complement(21463..22320)
                     /gene="yibB"
                     /locus_tag="ACHJ48_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO4708470.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
BASE COUNT         5721 a         5752 c         5628 g         5251 t
ORIGIN      
        1 ataatatatt catacaaaca cagacatctt ttgaaacgaa tgatacattt ttataatgaa
       61 tgcttaaaac aatatttaat cgacacacat cactgtcatt aatgtaaagc aacatagatc
      121 accaataata caatggacaa ctctaccggc attgatcgct gggcggagga aataaggatt
      181 tttacatgaa ctagaagaag atttttttca gtgctgtcac tgtttcagtt ctctgcgcat
      241 taacgggatg tgactacatt gaagaaggca agccagaaag tagtttattg aaacaacaag
      301 aagaacataa caataaaatt gatctacttg aaaaacaaca agcacagcta aagagtcaac
      361 tggaaactat tcaaaaacaa caaactggca taataagcag tacaaagact ttaactcacg
      421 taattaaatc cgttaaagat caacaaaaca cttttatttt taccgagttt aacccagcaa
      481 aaacgaagta tttcatcctg aacaatggtt cggtggcttt agcgggtcga gtgttatcca
      541 ttgacgccac agagaatggc agtgttattc atatttcact ggtcaactta ttaagtacac
      601 ctatctcaaa tattggtttt aatgcgacat ggggtggtga aaaacctgtc gatgccaaag
      661 agtttgcccg gtggcaacaa ttacttttca acacatcaat gaaatccaca ttgaaattat
      721 taccaggtca atggcaagac attaatttga ccctgaaggg tgtatcgccc aataacctgg
      781 gatatctgaa gttagccatc aacatggaaa atattcagtt cgacaatctt ccctctgctg
      841 aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt acgcttataa caaggaatag
      901 cgagcatcat taatgaacaa aatatttaaa gttatctgga accctgcgac agggaattat
      961 actgttacca gcgaaacggc aaaaagccgt ggcaagaaat ctgggcgcag taagctgtta
     1021 atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg gggcattggc gaatgccggg
     1081 aatgacaacg gtcagggtgt tgattacggt agtggatcag ctggcgacgg ctgggttgct
     1141 ataggcaaag gggcgaaagc aaatactttt atgaacacca gtggttccag tactgctgtg
     1201 ggttatgacg ctatagctga aggccaatat agctctgcca tcgggtcaaa aacccatgcg
     1261 attggtggtg catcaatggc ctttggggtt agtgcaatat cagaaggcga tagaagtata
     1321 gcactgggtg cctcttcgta ttcattgggc caatactcaa tggccctcgg ccgttattca
     1381 aaagcattgg gtaaattgtc tattgctatg ggggactctt ccaaagcgga aggagcaaac
     1441 gccattgccc tgggaaatgc cactaaagct actgagatta tgagtattgc tcttggcgac
     1501 accgccaatg cgtcaaaagc gtattcaatg gcgctgggag caagtagcgt cgcatctgaa
     1561 gaaaacgcta ttgcgatagg tgctgagacc gaagccgctg aaaatgcaac tgctattggc
     1621 aataatgcga aggcaaaagg gactaatagc atggcaatgg ggttcggaag ccttgccgat
     1681 aaagtcaata ctatcgcatt aggaaatggc agccaggctc tggcagataa tgcaatcgcc
     1741 ataggccagg gcaacaaagc tgatggcgtg gatgccatcg ctctgggtaa tggtagccag
     1801 tcgagaggct taaacaccat tgccttaggc acagccagta atgcaactgg tgataagagt
     1861 cttgcgcttg gtagtaatag cagtgccaac ggtattaact ctgtcgcgct gggcgcagat
     1921 tccattgcgg atttagacaa taccgtctct gtcggcaata gttcattaaa acgcaagatc
     1981 gttaatgtga aaaatggcgc gatcaagtct gacagttacg atgccattaa tggttcacag
     2041 ctttatgcca ttagcgactc ggtagcaaaa aggcttggag gaggggctgc agtagatgtt
     2101 gatgacggta ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta
     2161 ggggctgcgc tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa
     2221 tacagcgctg ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat
     2281 ggcacgattt cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat
     2341 gacgatgtcg aagccaacac cgccaatatc gctactaata ccagcaacat tgccacgaat
     2401 acggcaaata ttgccaccaa taccaccaat atcaccaacc tgacggattc cgttggtgac
     2461 cttcaggctg atgccctgct ctggaacgaa actaaaaagg cattcagtgc agctcacggc
     2521 caggatacca ccagcaaaat caccaacgtt aaagatgccg acctgacggc tgacagcact
     2581 gatgctgtta acggctctca gctgaaaacc accaacgatg ctgtggcgac gaataccacc
     2641 aatatcgcca ataacacttc caatattgcc actaacacca ccaacatctc taacctgact
     2701 gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtattc
     2761 acggcagctc atggcaccga gaccaccagc aaaatcacca acgttaaaga tggcgacctg
     2821 acgactggca gcaccgatgc cgttaacggc tctcagctga aaaccaccaa cgatgccgtg
     2881 gcgacgaata ccaccaatat cgccactaac accaccaaca tctctaatct gactgagacg
     2941 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt cttcactgca
     3001 gctcatggca acaataccgc cagcaaaatc accaatatcc tggacggcac agtcactgca
     3061 accagttccg atgccattaa cggtagccag ctttatgact taagcagcaa tatcgccacc
     3121 tacttcggcg gcaatgcttc tgtgaatact gacggtgtgt ttaccggtcc aacctacaaa
     3181 atcggtgaaa caaattatta taacgtcggc gatgcactgg ctgcgattaa ctcctcattt
     3241 agcacgtctc tcggcgatgc tctgctttgg gatgccaccg caggtaaatt cagtgccaaa
     3301 cacggtacta atggtgacgc aagcgtgatc actgatgtcg cagatggtga aatttcagac
     3361 tccagttctg acgcagtaaa cggctcacaa ctccacggcg tgagcagtta tgttgttgat
     3421 gcgctggggg gtggtgccga agtcaatgca gacggcacca tcactgcgcc gacgtacacc
     3481 attgctaatg ctgattacga taatgtcggt gatgccctga atgctatcga taccactctt
     3541 gacgacgctc tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag cgccgctcac
     3601 ggaaaagata aaactgccag tgtaatcact aacgtcgcta acggtgcaat ctctgctgcc
     3661 agcagcgacg cgattaacgg ctcacaactc tataccacca ataagtacat cgctgatgcg
     3721 ctgggtggtg acgcagaagt caacgctgac ggcaccatca ccgcaccgac ttacaccatt
     3781 gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga taacgccctg
     3841 ctgtgggatg agactgccaa tggcggtgct ggagcctaca atgccagcca tgacggtaaa
     3901 gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac cgatgcagtg
     3961 aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca aattatcaat
     4021 cagctcgctg gtaacaccga cgcaacctat atccaagaaa acggtgcggg tattaactat
     4081 gtgcgtacta acgacgacgg cttagcgttc aacgacgcca gcgcacaggg tgttggcgct
     4141 acagctatag gttataactc tgtcgccaaa ggcgatagca gcgtagctat tggtcagggc
     4201 agctacagcg acgttgatac gggtatcgcc ctgggtagca gctctgtttc cagccgagtg
     4261 attgccaaag gctcccgtga caccagcata acggaaaatg gcgttgttat tggttacgac
     4321 accacggatg gcgaactgct cggtgcattg tctatcggtg atgacggtaa atatcgtcaa
     4381 atcatcaacg tagccgatgg ttccgaagcc catgacgccg ttacggttcg tcaattgcag
     4441 aatgcgattg gtgcggtcgc aaccacgccg actaaatact tccacgctaa ttcaacggaa
     4501 gaagattcac tggcagtggg aactgactcg ctggcaatgg gtgcgaaaac catcgtgaat
     4561 ggcgataaag gtattggtat cggttatggt gcctacgtgg acgcgaatgc acttaacggc
     4621 attgccattg gtagcaatgc gcaagtcatt catgtcaaca gtattgcgat aggtaatggt
     4681 tctacgacca ctcgtggcgc tcaaaccaat tataccgcct acaacatgga cgcaccgcag
     4741 aactctgtcg gtgaattctc agtcggtagt gcggatggtc aacgtcagat cactaacgtc
     4801 gcagcaggtt cggctgatac cgatgcggtc aacgtgggtc agttgaaagt aacggatgcg
     4861 caggtttccc agaataccca gagcattact aacctggata atcgggtaac gaatcttgat
     4921 tcacgcgtca ccaatatcga aaacggtatt ggcgatatcg tcaccaccgg tagcaccaag
     4981 tacttcaaga ccaataccga tggtgtagat gccagcgcgc agggtaaaga tagcgtcgcg
     5041 attggttccg gctccattgc tgccgctgac aacagcgtcg ctctgggtac agggtctgtg
     5101 gcaaccgaag aaaatacgat ctctgtaggt tcctctacta accaacgtcg tatcaccaac
     5161 gtagctgcag gtaaaaatgc taccgatgct gttaacgtgg cacagttgaa gtcttccgaa
     5221 gctggcggtg tacgttacga caccaaagct gatggttcta tcgactatag caatatcacc
     5281 ctcggtggcg gcaacggcgg tacgactcgt atcagcaacg tctccgctgg cgtcaacaac
     5341 aacgacgtgg tgaattacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
     5401 gatcagcgaa tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa gttgagcggt
     5461 ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttacactcc aggtgccagc
     5521 atggcctcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt aggtgtatcg
     5581 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
     5641 gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa gttagtgtga
     5701 ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat aaggcgctcc
     5761 gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta ccagacatct
     5821 ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt tcacaattcc
     5881 ccaattaaaa ctcacatcaa tattgccaat acataacatt tagttaacca ttcattgtca
     5941 ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac aaggagatga
     6001 gcaacagact cattacacga tgtgcgtgga ctccaggaga cctgcaatga atctctggca
     6061 acaaaactac gatcccgccg ggaatatctg gctttccagt ttgatagcat cgcttcccat
     6121 cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg ccgcctcatg
     6181 gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc
     6241 gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt ggatcattat
     6301 tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca tcattcgctc
     6361 gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg gtttctgttt
     6421 cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt
     6481 gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga ttgttaacac
     6541 cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac aagtaacagg
     6601 catcgacagc tttgagattg gccagatggt ggggcggcag ctaccgttta tgaccattat
     6661 cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaagg agacgtggcc
     6721 tgcggtcgtg gttgcgggcg gctcgtttgc catcgctcag taccttagct ctaacttcat
     6781 tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc tgacgctgtt
     6841 cctcaaacgc tggcagccag tgcgcgtatt ccgttttggt gatttggggg cgtcacaggt
     6901 tgatatgacg ctagcccaca ccggttacac tgcgggtcag gtgttacgtg cctggacacc
     6961 gttcctgttc ctgacagcta ccgtaacact gtggagtatc ccgccgttta aagccctgtt
     7021 cgcatcgggt ggcgcgctgt atgagtgggt gatcaacatt ccggtgccgt acctcgataa
     7081 actggttgcc cgtatgccgc cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa
     7141 gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc tctcgattgt
     7201 ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc tgaaagaact
     7261 ggctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct cgaactattc
     7321 cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat tcaccttctt
     7381 ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct catctaacgc
     7441 cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg atctgttgct
     7501 ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc aatctatcgc
     7561 tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc gctttactgt
     7621 caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc aggcttatgt
     7681 cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga cgaggttgcc
     7741 gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct
     7801 gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactacgcga ggcgctggca
     7861 aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg
     7921 cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct gatggccgat
     7981 gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc cagcaccgca
     8041 tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct ttgctttgaa
     8101 gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg
     8161 attgccgaag cctcacataa catcgtgctg ctgcaaacca tacgcggttt cttcgatgtc
     8221 ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc ggttttttca
     8281 caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga tgccgacggg
     8341 gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa acgattcgat
     8401 gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa tgagcattcg
     8461 agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca gcgcaacgca
     8521 ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa tacacgctgc
     8581 gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt
     8641 ctgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg gtagcactgg
     8701 caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg
     8761 cggacgcgca tggcattccg tttactctct caacggtttc cgtttgcccg attgaagaag
     8821 tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc gatcgcggct
     8881 ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg
     8941 tggatatgcc gacaccggga gcgcgttatc gtgatgcgca ttctgggatg agcggcccaa
     9001 atgcggcaat gcgccgctac ttgcaagcgg tgacgcatcc gcaatgggcg tgggatgtgg
     9061 gcctgaacgg tcgtccgcat gatttaggta atatttcggc ttacctcggc aaaccgaccg
     9121 gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc tcatggaaag
     9181 accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg atcctcgatc
     9241 cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg gattgtagtt tctaaccacg
     9301 gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct attgcagatg
     9361 cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg cttgatgtcg
     9421 tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc ctgtatgcgc
     9481 tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgag aaagagatga
     9541 aagtggcaat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa gattcgctgg
     9601 tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa gggaatgcgg
     9661 catagtcgtt tgagccccct caccctaacc ctctccctca gggagtgggg accgttcggc
     9721 gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc ctcgcccctc
     9781 cggggtgagg ggaaaaggcc gcactgctca ccaattttct gctatcctgc ccccgcacta
     9841 agggggcagt atgctaaaca tcgtacttta cgaaccagaa attccgccaa atactggcaa
     9901 tatcatccgt ctttgcgcta ataccggctt tcgtctgcat atcatcgaac cgatgggatt
     9961 tgcctgggac gataagcgcc tgcgccgcgc ggggctggac tatcacgagt ttaccgccgt
    10021 tacgcgtcat catgactttc gcgcgttcct cgaagcagaa aatccccagc gcctgtttgc
    10081 cctcaccacg aaaggtacgc ctgctcacag cgccgtaaac tatcaggatg gcgattatct
    10141 gatgttcggc ccggaaacac gcggcctgcc agcgagcatt cttgatgccc tgcccgctga
    10201 acaaaaaatt cgcattccga tggtgccgga cagccgcagc atgaacctgt ccaatgcggt
    10261 gtcagttgtg gtgtatgaag cctggcggca gctgggatat ccgggagcgt tgttgagaga
    10321 ttagttactg tatgccggat agatgattac atcgcatccg gcacgatcac aggacattag
    10381 atcccatccc catactcaaa tgtatggtta ataccgttga aatgctggtc catatccatt
    10441 gatggcttat cgctgtctgg tttaccgaca atacgagccg gaacgccagc ggcggttgta
    10501 tgcggcggca ccggttgcag caccacggaa cctgcgccaa tcttcgcgcc gcgaccaact
    10561 tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca caccttcacg aattttcggg
    10621 tgacggtcac caccagattt acccgtaccg ccaagcgtca cagattgcag aatcgatacg
    10681 tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg tggcgtggtc gagcataatg
    10741 ccacggccaa tttttgctgc cgggtgaata tcgacctgga acgtcacaga aacctggttt
    10801 tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca accagtgacc gatgcgatag
    10861 gcctgcaagg catgaaaacc cttcaggtat aacaacgggg ttgagtattt atcgactgcc
    10921 gggtcgcggg tacgcaccgc ctgaatatca caggccgcag aggcgatcat ttccgggtca
    10981 gcggcgtagg cttcttccac cacttcacgg atagcaatag caggcataat tggcgatgac
    11041 agcttgttcg ccagcatgta gctcagtgca ctaccaaggt tttcgtgctt gagtagcgtc
    11101 gcgtggtaaa aactggccag cattggctca cagtccgcca gcgttctggc ttcggcttta
    11161 atattgttcc agacaatttc cagttcttca cacgacattg cttactccac acgatgagat
    11221 aatgaccggc ccgttctgcg cgggccgggt catagcggta acaaaggttc cctggggtta
    11281 gtggctgctg cgctcgtcct tgcgtgcacg acctaataac gtcaatgctg cctcgcgcgc
    11341 gttttttccg caatataata cttgataaat ttcctcggtt attggcattt ctacgccgaa
    11401 gcgatgcgcc agttcgcgga cttctttcgt attgcggtag ccttccacca cctgaccaat
    11461 cttctcctgc gcgctttgta catccatgcc ctgaccgagc atcatgccaa aacggcggtt
    11521 acgcgactgg ttgtcggtac aagtaagcac aagatcgccc agccccgcca tgcccataaa
    11581 ggtggcagga tcggcaccca gcgccgcgcc aagacgcgac atttcagcca gcccacgggt
    11641 gatcagtgcc gtacgcgcat tcgcaccaaa accgataccg tcggacatcc ccgcgccaat
    11701 ggcaatgacg tttttcaccg caccgccaag ctgcacgcca atgaaatccg gattgctgta
    11761 aacgcggaaa cttttgccgc agtgcagcag ctgctggaga tcatcggcaa aggtctgatc
    11821 ggtcgaggcc agcgaaatag ctgtcggtaa acctgccgcc agttctttcg caaacgttgg
    11881 gccagagata accgccagcg gaatttgatc gcctaaggcc tcacgcgcca cgtcctgtaa
    11941 cagacgtccg gtttccgctt ccagcccttt ggtcgcccac accagacgcg catcaggacg
    12001 catcagcggt ttaatctggc gcagcacttc accaaagaca tggctgggta cgacgacgag
    12061 aatattacgg ctggctgcca gcgcagtggc gagatcgctt tcaagatgga gcgtatcggg
    12121 aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg ttgcgatatg
    12181 ttcagggtca tggccccaga ggacaacctc gtggccattt cttgccaggg tgatggcaag
    12241 agcggtgccg tacgagccgg caccgatcac agtcattgaa gcattacgtt ggttcatcag
    12301 gcatcctgat gttcttcagt accttcgcca gcctgctgct gcaaatagtt catgaacagc
    12361 gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg taccgcggga taccatgctg
    12421 gtgatgcact cacgagcata cgggaacaga atgttcgggc agtatgcgcc caggcaatgt
    12481 gccatctggg tgccttcgat acccgcgatg gagaaaatac cgccctgctg aacttcacac
    12541 aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa cacgcagtac cacttcgtat
    12601 acgtcatctg ccagttggga agatgccgta tccagatcaa gtttaacttc tggttgccaa
    12661 tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag agatatcctt ggtataaata
    12721 cgttggatct ggaaagtcat ttcagtgttg ttttgttctg acatgtgtag aaaaccctta
    12781 agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt cacacgttat ttcagcaggg
    12841 gatccagtcc accacgtgca tccaatgcat acaagtcatc acagccgcca atgtgctgtg
    12901 cgtcaataaa aatctgggga accgtggtgc gaccgctgcg tttgatcatc tcttcacgct
    12961 tggcggcatt gccatcgatc ggcaactcct gaaaactcac gcccttgctg ctcagcagtg
    13021 cttttgcacg atggcaatac gggcaggttt ctttggtata gatttcaaca ttggccataa
    13081 tttagctcct gtttttttac cctgaaattt catgttgcag ggcggctgca aaatggagtg
    13141 tccccagaag cttataacaa taagtcgctg ggggcctaat tgcagctaac ggcctgcatc
    13201 atgaaagacg acaggtaaat tatttgccgc gcaccaaagg caagttttcg ccagcccagc
    13261 cagcgacgcc ttctttcagc acgaatactt gcgcaaaacc agctttcgtc agtgcgtttg
    13321 caggctcctg gcactgcatg ccagaaccgt ctaccacgat aaccggtttg tctttgtgct
    13381 tctcaagctc accaacattg ttggctttga tttcgctcgg caacagattg atagaacctg
    13441 cgatatggcc tttacggaag tcatcacgct gacgtaaatc cacaaccaca gcgtcttctt
    13501 tgttgatcag acgcgtagct tcaccacgag taatcacctt cactttcgag gtcaggctct
    13561 taaacgtagt cacaagaacc gccaccagta acgcgatcca ggcgatactc agtatgggat
    13621 gacggccaac aaattgcata atttcttgca tggggggtaa caactcccga cgtagtgatt
    13681 aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa atacagccag cgcgttaact
    13741 ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa atctggcatc ggcttgccgc
    13801 gagcggtcgt atattttgat cttcagaggc tattttatcg attcagctgt agtaaaatta
    13861 cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc ggtttctaaa aaacctatgg
    13921 tactggtgat tctggatggc tatggctatc gtgaagaaca gcaggataac gccattttta
    13981 gtgctaaaac cccggtaatg gatgcactgt gggccaatcg tccgcatacc ctgatcgacg
    14041 cttccggtct ggaagtcggt ctgcctgacc gtcagatggg taactccgaa gtaggccacg
    14101 ttaacctggg tgccggccgc atcgtgtatc aggacctgac tcgtctggac gttgaaatca
    14161 aagatcgcgc tttctttgct aatccagtgc tgactggcgc ggtagataaa gcgaaaaacg
    14221 caggtaaagc agtacacatt atgggtctgc tctccgcagg cggcgtacac agccacgaag
    14281 atcacatcat ggcgatggta gaactggcag ctgaacgcgg cgcagaaaaa atctacctgc
    14341 acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc tgaatcctcg ctgaaaaaat
    14401 ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc gtccatcatt ggtcgttact
    14461 acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa agcctatgac ctgctgactc
    14521 tggcgcaggg tgagttccag gccgatactg ccgttgctgg tttgcaggca gcttatgctc
    14581 gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg tgcagaaggt cagccagatg
    14641 cggcaatgga agacggcgat gcgctgattt tcatgaactt ccgtgctgac cgcgcgcgcg
    14701 aaatcactcg tgcattcgta aacgcagatt tcgacggctt cgcgcgtaag aaagtggtta
    14761 acgtcgattt cgtgatgctg accgaatacg ccgctgacat caaaactgcg gttgcttacc
    14821 cacccgcttc cctggttaac accttcggcg agtggatggc gaaaaacgac aaaactcagt
    14881 tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt cttcttcaac ggtggcgtag
    14941 aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc accgaaagtg gctacctacg
    15001 atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa actggttgcg gccatcaaga
    15061 gcggcaaata cgacaccatt atctgtaact atccgaacgg cgacatggta ggtcacaccg
    15121 gggtgatgga agcggcggtt aaagcggttg aagcactgga tcactgcgtg gaagaagtcg
    15181 cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac cgctgaccac ggtaacgctg
    15241 aacagatgcg cgatccggca acgggtcagg cacacacggc acacaccaac ctgccagttc
    15301 cgctgattta cgttggtgat aagaacgtga aagcggttgc aggcggcaaa ctttctgaca
    15361 tcgcgccgac catgttgtcg ctgatgggta tggaaatccc gcaagagatg actggtaagc
    15421 cgctgttcat cgtggaataa tccctcccca tgaggggaaa ggcgattaat accatgacac
    15481 gggccgtgaa accgcgcagg tttgcaatca ggcccatcat ctacgccagc gttcttagcg
    15541 ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga gcgtgaccaa ctcaaatcta
    15601 ttcaggccga tatcgccgca aaagagcgcg cggtacgcca aaagcaacaa caacgcgcaa
    15661 gcctgctcgc acaattgaaa aagcaggaag aagcgatctc tgaagccacc cgtaagctgc
    15721 gcgaaacgca aaacacgctc aatcaactca ataaacagat tgatgagatg aacgcgtcga
    15781 ttgccaaact ggagcagcaa aaagccgccc aggagcgcag cctcgccgca caactggatg
    15841 ccgcattccg tcagggcgag cataccggta ttcagctgat tctcagcggt gaagaaagcc
    15901 agcgtggaca gcgtttacag gcttatttcg gctatctcaa ccaggcgcga caagaaacca
    15961 ttgcccagtt gaagcaaacg cgtgaagaag tcgccatgca gcgtgctgaa ctggaagaga
    16021 aacagagcga gcaacaaacg ctgttatatg agcagcgcgc ccaacaggcg aaactgactc
    16081 aggcgctgaa cgagcgtaaa aagacgctgg cagggctgga gtcttccatc cagcaaggtc
    16141 agcaacagtt gagcgagctg cgcgccaacg aatcccgtct gcgtaacagc attgcccgtg
    16201 cggaagccgc ggcgaaagcg cgtgcagaac gagaagcacg tgaggcccag gcggttcgcg
    16261 accgccagaa agaagcgacg cgcaaaggca ccacctacaa accgaccgaa agcgaaaaat
    16321 cgctgatgtc ccgaactggt ggcctggggg cgccgcgtgg tcaggcattc tggccggttc
    16381 gcgggccgac gctgcatcgc tatggtgaac agctacaggg cgaactacgc tggaaaggaa
    16441 tggttatcgg tgcctctgaa ggtactgaag ttaaagcgat tgccgatggt cgggtgattc
    16501 tggctgactg gctgcaaggt tacggtctgg tggtggtggt tgagcacggt aaaggcgaca
    16561 tgagtcttta cggctataat cagagcgcac tggtgagcgt tggttcgcag gttcgcgcgg
    16621 gccagccaat tgcactggtg ggcagcagtg gcggtcaggg tcggccttca ctctatttcg
    16681 aaattcgccg ccaaggtcag gcggtcaatc cacagccgtg gttgggaaga taagttttgt
    16741 ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt ggcgctctcc tcccccgtac
    16801 ttgctggcaa acttgccatc gtcattgatg attttgggta tcgcccgcac aacgaaaacc
    16861 aggtgctggc gatgccctcc gctatctccg tcgctgtatt acccgattca ccgcacgcca
    16921 gagagatggc gaccaaagcg cataacagcg ggcacgaagt gttgattcat ctcccgatgg
    16981 caccactgag taaacagccg ctggagaaaa atacgctgcg cccggagatg agcagcgacg
    17041 aaattgagcg cattattcgt agtgcggtca ataacgtgcc ctatgccgtg gggatcaaca
    17101 accacatggg tagcaagatg acctctaacc tgtttggtat gcagaaagtg atgcaggcac
    17161 tggagcgtta caatctttac ttcctcgaca gcgtaaccat cggtaatacc caggcgatgc
    17221 gcgccgcgca aggtactggc gtgaaggtga tcaaacggaa ggtgttcctc gacgattcgc
    17281 agaacgaagc ggacatccgt gtgcaattta atcgcgcaat tgatctggcg cgtcgcaacg
    17341 gttcaaccat tgccattggg catcctcacc cttcaacggt acgcgtgcta caacagatgg
    17401 tttataacct gccgccagac attacgctgg tgaaagccag cagcttgctg aatgaaccgc
    17461 aggttgatac ttctacaccg ccgaaaaacg ctgtgcctga tgcaccgcgt aatccgttcc
    17521 gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc tgtttatgct aatcgcttct
    17581 ttgaagtatt aagcgaaagc atcagccaga gcacgctgat cgtttacttc cagcatcagt
    17641 ggcaaggctg gggcaaacag cccgaagcgg cgaagtttaa cgctagcgca aattaaaggc
    17701 gctacgcgta atgcgatggt gcgaccgcgt ttttttcgcg ccattgccat aagcggaaag
    17761 accacagtaa cgcctggtaa ccgaccttca cgctgcgcac attggtaatc aggcgcttat
    17821 acataccgga agtaaatatc tcggcaatca tccgttggcg ggtaagaata tccggctctt
    17881 tgcgcaccgc atggcaaaca cgcaatgctt cgtaagttat ttgctgatga aattccggat
    17941 aaatcataat tttgtcggca taatttcgat ttaatttctc cagcaggcgg gtaatcttaa
    18001 tatagtgacg ttgataatta aggtttttat tcccttgtct atgcaaccga ctcactgacg
    18061 tattatgcag ataatattta tataatgatt gctcggtata tcgcgcacgc agcgcgttaa
    18121 acatgaattc tgttgtccag acaatatcct gatgatgtaa tccggcaata aatttaatgt
    18181 tatttttaac aataacatca cggcgataaa cccccatcca gacaacgtga gtccaacggc
    18241 gcgaagaaag ccccatccgc agccagtccg ggccggttaa tacaccggtt gagcgaaggc
    18301 gatcgctggg gatggattgc caggtttctc ccgtttcacg aaaagaccag tcagcgttgc
    18361 actgcgccac gtcgaggtcg tcctctaacg ccatggtcat cagcgtttcg tacatggtgg
    18421 gatagacctc atcgtcagca tcgacaaaag cgacatattt gcccgtcgcc acctcgatcc
    18481 cacgattacg cgctaccgat gcgccagcat tcgcctgatg caacaaacga acgtgcggat
    18541 agttttctgc gtaatgcttt gctatttcaa cagaattatc cgttgaacca tcgttaataa
    18601 taatgatttc cagagcagtc caggtttgcg taattaaaga ttccatacaa gtgcggaaat
    18661 catcgcccgc attatataac ggaataataa cactaagttt attggtgctg ttcatcataa
    18721 attaccgacg acctctgaag ttatcctgac gttttacagg ctttgtatta agaaactatt
    18781 aagcctgtga gatattattt ttacaggtga gagagtcact tcagggtaga gagataatga
    18841 gaacagctat ataaaagatc agcgcaatta cgaactcttt attacactga caggatttta
    18901 catatttaac agattatcct caggcaaaag ggctggaata ccagcccttt ttcgtgttaa
    18961 tcccagctca gaataacttt cccggactgg cccgaacaca tagcgtcaaa gcccttctgg
    19021 aaatcatcga tagagaaacg atgggtaatg atcggcgaga gatcgaggcc agactgaatc
    19081 agcgccgcca tcttgtacca ggtttcaaac atctcacgac cgtaaatgcc tttaatgaac
    19141 aagcctttaa agatcacttt ggtccagtcg atagacatgt cagacggtgg aatacccagc
    19201 atcgcaatac gaccgccgtg gttcatggta tcgagcatgg tacgaaacgc tggcggcgcg
    19261 ccggacattt ccagaccgac atcaaagcct tcggtcatgc ccagttcagc catcacatca
    19321 ttaaggtttt ctttcgcgac gttaaccgca cgggtgatac ccattttgcg cgccagctca
    19381 aggcggtatt cgttaacatc ggtgatcacc acattgcgtg caccaacgtg tttcgccacc
    19441 gccgcagcca taatacctat cggacctgca ccagaaacca gcacatcttc gcccaccaga
    19501 tcgaacgaca gcgcggtatg cacggcgtta ccgaagggat caaaaatgga agccaggtcg
    19561 tcggagatat tgtcggggat tttgaaagcg ttgaacgccg gaatcaccag atattcggcg
    19621 aagcagcccg gacggttaac accgacacca atggtgttgc ggcacagatg ggtacgaccg
    19681 ccacgacagt tgcgacagtg accgcaggtg atatggcctt cgccagaaac gcgatcgccg
    19741 attttgaagc ctttcacttc ctgaccaata cctaccactt caccgacata ttcatggcct
    19801 acgaccatcg gcaccgggat ggttttttgc gaccactcat cccagttata gatgtgaacg
    19861 tcagtcccgc agatggctgt tttacggatt ttaatcagca gatcgttatg cccgagttcc
    19921 ggtacaggaa cgtcggtcat ccagatgccc tcttccgctt tcagtttgga taacgctttc
    19981 atctcacatc ctcaggcgat aacgcccagt tgtttaccaa tacgcgtgaa tgcttctact
    20041 gcacgcgtaa tttgctcagg ggtatgcgcc gcagacatct gggtacgaat acgcgcctga
    20101 cctttcggaa cgaccggata gaagaaaccg gtgacgtaaa tgccctcttt ttgcagctca
    20161 cgggcaaatt tctgcgccac tactgcatcg ccaagcatga ccggaataat ggcatgatcg
    20221 gctcccgcca gggtaaagcc cgccgccgac atttgctcac ggaactggcg cgcgttcgcc
    20281 cacagacggt cacgcagttc gctgcccgct tcgaccatct ccagtacttt gatggacgcg
    20341 gcaacaatgg ccggtgccag cgagttggag aacaggtacg gacgagaacg ctggcgcagc
    20401 cactcaacca cctctttgcg cgccgcggta taaccaccag aagccccgcc cagcgcttta
    20461 ccaagcgtac cggtgataat atcgacccgg cccatcacat cgcagtattc atgggaacca
    20521 cgaccatttt caccgacaaa accgaccgcg tgggagtcat ccaccatcac cagggcatcg
    20581 tatttatctg ccagatcgca aacgcctttc aggttggcaa tcacgccgtc cattgagaac
    20641 acaccatcgg tggcaatcag cacatgacgc gcaccggctt cacgcgcttc tttcagacgt
    20701 gcttccagct cctgcatatc gttgttggca tagcgatagc gtttagcttt gcacagacgc
    20761 acaccatcaa taatagacgc gtggttcagt gcgtcggaga taatggcgtc ttccgcaccc
    20821 agcagcgttt caaacagtcc accgttagca tcaaagcagg aagagtagag aatcgcatct
    20881 tccatcccca ggaatgccgc cagtttttgt tcaagctctt tatggctgtc ctgagtgccg
    20941 caaataaaac gcaccgaagc catgccgaaa ccgtgagaat ccattcccgc ctttgctgcc
    21001 gcaatcagat ccggatgatt cgccagcccg agatagttgt tggcacaaaa gttaatgacg
    21061 tggcttccat cagccacagt gatatctgct tgctgcgcag acgtaataat gcgctcttct
    21121 ttaaacaacc cttccgcccg tgcggtttcc agatcgttgg ttaactgctg ataaaattct
    21181 ccacgcattg cgattctcca gacagggcaa attccagcac atattaccca aacttatagg
    21241 tcgcgacgag atgacgcgtt aactcttctg caaaattcag gataaatgac gtgtaacttg
    21301 atgtctctca acacctacag atagtagaaa aactgtacag ttttggctgg gcataatgat
    21361 gattgatttt actgcattga tataaaaaga aaaaatgatg ccaaagcatc atttttccta
    21421 tctcagttgg tatcaaaacg ctacccacta atggagggaa acctattttc ttgaaaggaa
    21481 aattctcagc gcctgcattt ttgcccctaa agtgttactc ctgaagcagc gaaacaaatc
    21541 gaaccatttt cctctcccca gatagtttag gttgaagaga tcggggcgtt tgtaatagca
    21601 catgacaaaa atgccctggt catcatccac aatgttatta tttagtgtta ttttttgact
    21661 ttccagaact aatttataga attctttcca tttatgctgt gaaccaacaa ttgcgccccc
    21721 aataatatat acatgattgc caatcatgaa atcaaaaacc tgttgttgag atgtgacagt
    21781 aagccctttt tttatagtga aaagatgcat cttactttca tcaaatggga aatcccaaat
    21841 tttcaatcct cgagttacat ttggcttacg gcaatagcca aaatctatcc acgcaacaag
    21901 aggcgttttc actaagccca tgttgatagc tttatttaca aaataggcct taaggttaca
    21961 taccaaaaca tactctggtg accagtactc tgggtttttt aattgtcgag gctcaagtct
    22021 atttgtaaac gactcatctt tttgaatttt ttcgatacga cttctgatat atctaaattt
    22081 cttttttata tcgataacga taaccgttgt tggttttccg ttgcgaatcg cttcaaccct
    22141 tggtttcaaa tcgggagaag tgaaaataat catctcattt tcaagtgcgg ccagacgctc
    22201 aaagtaacta aaataaacat caactgaacg agctagtttt tcacggaacc ctttattagc
    22261 agtccaatcc cctctaccaa tgtcaaaata tgcagtgata attgtggtgg atgatttcat
    22321 tactaataag accattaata taaaaaaaga at
//
LOCUS       DAZFDC010000076        22325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFDC010000076 DAZFDC010000000
VERSION     DAZFDC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.076"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            95..1195
                     /gene="torY"
                     /locus_tag="ACHJ48_002959"
     CDS             95..1195
                     /gene="torY"
                     /locus_tag="ACHJ48_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO4708471.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLMAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKLLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            1220..3649
                     /gene="torZ"
                     /locus_tag="ACHJ48_002960"
     CDS             1220..3649
                     /gene="torZ"
                     /locus_tag="ACHJ48_002960"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO4708472.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGYNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAQLRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(3814..4785)
                     /gene="cmoB"
                     /locus_tag="ACHJ48_002961"
     CDS             complement(3814..4785)
                     /gene="cmoB"
                     /locus_tag="ACHJ48_002961"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO4708473.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4782..5525)
                     /gene="cmoA"
                     /locus_tag="ACHJ48_002962"
     CDS             complement(4782..5525)
                     /gene="cmoA"
                     /locus_tag="ACHJ48_002962"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO4708474.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(5566..5961)
                     /gene="yecN"
                     /locus_tag="ACHJ48_002963"
     CDS             complement(5566..5961)
                     /gene="yecN"
                     /locus_tag="ACHJ48_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO4708475.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(6014..6811)
                     /gene="yecE"
                     /locus_tag="ACHJ48_002964"
     CDS             complement(6014..6811)
                     /gene="yecE"
                     /locus_tag="ACHJ48_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO4708476.1"
                     /translation="MEPDSEIISLNISCFSGSCRHINCVEGNTTLYALPKPEVVLRWR
                     EQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPR
                     ELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNQVILDSRPVHA
                     ARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQ
                     TTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            complement(6795..8081)
                     /locus_tag="ACHJ48_002965"
     CDS             complement(6795..8081)
                     /locus_tag="ACHJ48_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4708477.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEITRLGPQYQIDVKLREFGRTARHECTFVFNPQLVKKCQQVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            complement(8115..8369)
                     /gene="xisR"
                     /locus_tag="ACHJ48_002966"
     CDS             complement(8115..8369)
                     /gene="xisR"
                     /locus_tag="ACHJ48_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4708478.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(8425..8529)
                     /locus_tag="ACHJ48_002967"
                     /pseudo
     CDS             complement(8425..8529)
                     /locus_tag="ACHJ48_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(8561..8932)
                     /locus_tag="ACHJ48_002968"
     CDS             complement(8561..8932)
                     /locus_tag="ACHJ48_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO4708479.1"
                     /translation="MNNQNTQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVINCAIESLYATLPYEEVLDERTGKTDIMATINIGELICQDEDLSGELW
                     LAGMLISAEIISIEPATNIRL"
     gene            complement(8973..9800)
                     /locus_tag="ACHJ48_002969"
     CDS             complement(8973..9800)
                     /locus_tag="ACHJ48_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4708480.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITATDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGVQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKALAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQIK"
     gene            complement(9797..9985)
                     /locus_tag="ACHJ48_002970"
     CDS             complement(9797..9985)
                     /locus_tag="ACHJ48_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708481.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTIAVTSGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            complement(10170..10742)
                     /locus_tag="ACHJ48_002971"
     CDS             complement(10170..10742)
                     /locus_tag="ACHJ48_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708482.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPGPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLITYPDKGF
                     IVSDMGREGLELLLDEMINDLNTHGMPEGQ"
     gene            complement(10748..10929)
                     /locus_tag="ACHJ48_002972"
                     /pseudo
     CDS             complement(10748..10929)
                     /locus_tag="ACHJ48_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11128..11328)
                     /locus_tag="ACHJ48_002973"
     CDS             complement(11128..11328)
                     /locus_tag="ACHJ48_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708483.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHNLLCAVIVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            complement(11325..12026)
                     /locus_tag="ACHJ48_002974"
     CDS             complement(11325..12026)
                     /locus_tag="ACHJ48_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4708484.1"
                     /translation="MEALQTTPKTCSLNTYNKVLCDDLDLDSFALTIANLLSAVRTFN
                     LLDDTRLKEVGFDVLEFTHEYALAIASTKQQHSIRSGNKIACIRTKREACGLTTTELA
                     RLLDLDEEIILQWESGEFEPTISMLIPLANVLGCDPLSLLSEKNSESAIRVNTTEVHA
                     ESIGARIKSARTKLGLTESDLARMIHTYSDPINDWECGICEVPADHIVPLASALNCDL
                     MWLLTGKSEAKEQQQ"
     gene            complement(12174..12863)
                     /locus_tag="ACHJ48_002975"
     CDS             complement(12174..12863)
                     /locus_tag="ACHJ48_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4708485.1"
                     /translation="MKNVKSTENRIAMMLKTKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRSDKLAQLSVISGYPQSWFLGEDASSTFSSAEKHHTREDSVVFNVLDVEFSCGDGT
                     HVRGDLIDVVRSIELDPEYARRLVGNRAFKNIEIGNARGDSMAPTISPGDLLFLDKTV
                     TYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQPWSIEKEGMALLYIQSKVI
                     SSVPFNINRFG"
     gene            13020..13253
                     /locus_tag="ACHJ48_002976"
     CDS             13020..13253
                     /locus_tag="ACHJ48_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4708486.1"
                     /translation="MLRKLIPQNAIARYIGVTPQAVNLWFSKNSVPSRFVLRVCELVE
                     WKVTPHGLRPDLYPYPEDGIPDSLRKSNGITRD"
     gene            13335..14042
                     /locus_tag="ACHJ48_002977"
     CDS             13335..14042
                     /locus_tag="ACHJ48_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4708487.1"
                     /translation="MNTAIFNDKASMTSVEIAELVGSQHKDVKRSIERLMGKGVIRSA
                     PMANFEIINNLGLKRNVAAYVFEGEQGKRDSIIVVAQICPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVIHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCCNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            14151..14813
                     /locus_tag="ACHJ48_002978"
     CDS             14151..14813
                     /locus_tag="ACHJ48_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO4708488.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGGRSPELLEAIYDAPASF
                     LSSMLAHARIMVGWAGEPKGSPVSLSAGTANPVQFTTHEFRSSGGGSFSLLKEAAIMA
                     TVPTSPYLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHEAEISLSHTAFRRNQWEV
                     RP"
     gene            14810..15043
                     /locus_tag="ACHJ48_002979"
     CDS             14810..15043
                     /locus_tag="ACHJ48_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708489.1"
                     /translation="MIRRVVNSLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQMVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            15030..15938
                     /locus_tag="ACHJ48_002980"
     CDS             15030..15938
                     /locus_tag="ACHJ48_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4708490.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETTRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQQPAAPGGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            15949..16827
                     /locus_tag="ACHJ48_002981"
     CDS             15949..16827
                     /locus_tag="ACHJ48_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4708491.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            16824..18224
                     /locus_tag="ACHJ48_002982"
     CDS             16824..18224
                     /locus_tag="ACHJ48_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4708492.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            18221..18478
                     /locus_tag="ACHJ48_002983"
     CDS             18221..18478
                     /locus_tag="ACHJ48_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708493.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            18530..19519
                     /locus_tag="ACHJ48_002984"
     CDS             18530..19519
                     /locus_tag="ACHJ48_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4708494.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            19538..19918
                     /locus_tag="ACHJ48_002985"
     CDS             19538..19918
                     /locus_tag="ACHJ48_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4708495.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            20134..20331
                     /locus_tag="ACHJ48_002986"
     CDS             20134..20331
                     /locus_tag="ACHJ48_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4708496.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            20482..21540
                     /locus_tag="ACHJ48_002987"
     CDS             20482..21540
                     /locus_tag="ACHJ48_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4708497.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRTLKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            21581..21656
                     /locus_tag="ACHJ48_002988"
     tRNA            21581..21656
                     /locus_tag="ACHJ48_002988"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21614..21616,aa:Ile,seq:cat)
     gene            21737..21813
                     /locus_tag="ACHJ48_002989"
     tRNA            21737..21813
                     /locus_tag="ACHJ48_002989"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21771..21773,aa:Arg,seq:tct)
BASE COUNT         5698 a         5205 c         5727 g         5695 t
ORIGIN      
        1 aggcagtgtt gtgccgaatt aagcaagtag tcaattcgtt gtgtatttat ttatacaatg
       61 atttcggtgt tctggaattt aattagagga atctatgcga gggaaaaaac gcattgggtt
      121 attgtttttg ctgatagcgg ttgtggttgg tggtggcggg ttcttgatgg cgcaaaaagc
      181 cttacataaa acgtcggata cagcattttg cctttcctgc cactcgatga gtaaaccttt
      241 tgaggaatat cagggaactg tccacttttc gaaccagaaa gggatacgtg cggaatgtgc
      301 cgattgccat attccaaagt cagggatgga ttatttattc gctaaattaa aagcatctaa
      361 agatatttat catgaatttg ttagcggcaa aatagacagt gacgataagt tcgaagctca
      421 tcgccaggaa atggccgaaa cagtatggaa agaattaaaa gcaactgact ctgcaacgtg
      481 ccgtagttgc cattcttttg atgccatgga tattgcctcg caaagtgaat ctgcgcagaa
      541 aatgcataac aaagcacaaa agggcggcga aacctgtatc gattgtcata aaggcattgc
      601 ccattttccg ccagaaataa aaatggatga caacgcggcg catgagctgg aaagtcaggc
      661 cgctacttca gtgactaatg gcgcacatat ttatcctttc aaaacttctc gcataggcga
      721 gctggctacc gtgaatcctg gtaccgatct caccgtcgtt gatgccagtg gcaaacagcc
      781 gatcgttctg ttgcagggtt atcaaatgca gggcagtgaa aacacgctct acctggcggc
      841 aggtcaacgg ctggcgctag ccacattaag tgaagaaggt atcaaggcgc tcacggtaaa
      901 cggggaatgg caggctgacg aatacggcaa tcaatggcgt caggcgtctt tacagggtgc
      961 gcttaccgat cccgcattag cggaccgtaa actgctatgg caatacgctg aaaaacttga
     1021 cgatacctat tgcgctggtt gtcatgcccc tattgccgcc gaccattaca ccgtcaatgc
     1081 gtggccgtcc attgccaaag gaatgggggc acgaaccagc atgagcgaaa acgaactgga
     1141 cattttaacg cggtatttcc agtacaacgc caaagatatt accgagaaac agtgatcctc
     1201 gttgatcagg gaggaagtta tgacattaac aagacgtgaa tttattaaac acagcggtat
     1261 tgccgctgga gcgctggtgg tgacatcggc agcaccgcta cccgcatggg cagaagagaa
     1321 gggcggtaaa atcctcaccg cgggacgttg gggagcgatg aacgtagaag tgaaggacgg
     1381 caagattgtt tcttcgacag gcgcgctggc gaaaaccata ccgaactcct tacagtctac
     1441 ggcggcggat caggtacaca ccacggcgcg tattcagcat ccgatggtga gaaaaagcta
     1501 tctcgacaat ccactgcaac cggcgaaagg tcgtggcgaa gatacctatg tacaggtgag
     1561 ctgggaacag gcattaaaac tgattcatga gcaacatgag cgtattcgta aagctaacgg
     1621 accatcggcc atttttgccg gatcttacgg ctggcgctct tctggcgtgt tgcataaagc
     1681 gcaaacctta ctgcaacgtt atatgaacct ggcgggcggt tattccgggc atagcggcga
     1741 ttattcaacc ggggcagcac aggtgatcat gccgcatgtg gtcggttctg tagaggtgta
     1801 tgaacagcag acctcctggc cgctgattct ggaaaacagc caggttgtgg tgctgtgggg
     1861 aatgaacccg ctgaatacct taaaaattgc ctggagcagt accgatgagc aagggctgga
     1921 atacttccat cagctgaaaa aatctggcaa accagtgatt gccattgatc ctatccgctc
     1981 tgaaaccatc gagttctttg gctataatgc cacctggatc gcgccgaata tgggcaccga
     2041 cgtggcactg atgttaggga ttgcgcatac cctgatgaca caaggcaaac acgataaagt
     2101 gttccttgaa aaatacacca ccgggtatcc gcagtttgaa gagtatctga caggtaagag
     2161 cgataatact ccgaagagcg cggcctggac ggcggaaatt actggcgtac cggaagcgca
     2221 gatagtcaaa ctcgctgaac tgatggcggc gaatcgcaca atgctgatgg caggctgggg
     2281 aattcagcgc cagcaatatg gtgaacagaa acactggatg ctggtgacgc tggcggcgat
     2341 gttagggcag attggtacac cgggcggcgg ttttggtttc tcttatcatt actccaacgg
     2401 tggcaacccg acgcgtgttg gcggcgtgct gccggaaatg tctgcggcga ttgccggaca
     2461 ggccagcgag gccgctgatg atggcggaat gacggcgatt cctgttgccc gtatcgtcga
     2521 tgcgctggaa aatcctggcg gcaaatatca gcataacggt aaagaacaaa cgtatccgaa
     2581 tatcaaaatg atctggtggg cgggtggcgg taactttacc catcaccagg atactaatcg
     2641 tctgatcaaa gcatggcaga aaccggagat gatcgtcgtt tctgaatgct actggaccgc
     2701 agctgctaaa catgcagata tcgtattacc gatcaccaca tcgtttgagc gcaatgactt
     2761 gacgatgacc ggtgattaca gcaaccagca tattgtaccg atgaagcagg ctgtcgctcc
     2821 gcaatttgaa gcgcgaaacg attttgacgt gtttgccgaa cttgcggaat tactgaaacc
     2881 aggcggaaaa gagatctata ccgaaggtaa agatgaaatg gcgtggctga aatttttcta
     2941 tgatgccgct cagaaaggtg cccgtgcgca acgcgtcact atgccaatgt ttaatgcctt
     3001 ctggcagcaa aataaactga tcgaaatgcg ccgcagcgag aagaacgaac agtacgttcg
     3061 ttatggtgat ttccgcgccg atccggtgaa aaatgcgctg ggtacgccaa gcggcaaaat
     3121 tgagatttac tccaaaacgc tggaaaaatt tggctataag gattgcccgg cacacccaac
     3181 ctggcttgcg cctgatgagt ggaaaggtac cgccgacgag aagcagttac agcttctgac
     3241 cgcacatccg gcacaccgtt tacacagtca gcttaactat gcgcaactgc gtaaaaaata
     3301 tgcggttgcc gatcgtgaac caatcactat tcacaccgaa gatgctgctc gctttggtat
     3361 tgcgaatggc gatctggtcc gtgtctggaa caaacgtggt cagattttga caggcgctgt
     3421 ggtgactgac gggatcaaaa aaggcgtggt atgcgtgcat gaaggtgcat ggccagatct
     3481 ggaaaatggc ttgtgtaaaa acggcagtgc gaacgtgtta acggcggata tccccagctc
     3541 gcagctggca aatgcctgtg ccggtaactc tgcgctggtg tatatcgaaa aatatacggg
     3601 taacgcaccg aagttaacgg cgtttgatca gccagctgtt caggcataag acgtgaaaag
     3661 cgcccggaaa cgggcgctga ggttatgcaa acataaagct gcctgatgcg ctatgcttat
     3721 caggcctaca tagtccctgc aatatattga attagcaagg ttttgtaggc tggataaggc
     3781 gttcacgccg catccggcag taataccaga cctttacggc ttgcgcgcaa tcagcaccgc
     3841 gcgtttaggc gcaggataac cttcaactgt cttactcgga tcatgtgggt cgaggaaatc
     3901 ggacagagac tcggtaacca tccattcagt gcgtcgctgc tcttctgtag tggtaacgct
     3961 cacatccgca atgcggatat ctacaaaacc acacttcttc agccagtttt tcagcgccag
     4021 cgcggaaggg atgaaataga cattacgcat ttgcgcgtaa cgatcgcccg gcaccagcac
     4081 cgtgttctca tcgccatcaa taaccagcgt ttcaagcacc agttcacctt cattgaccag
     4141 ttgatctttc aactgccaga gatgctccag cggtgaacga cgatgataaa gcacgcccat
     4201 cgaaaagacg gtatcaaagg ctttcagtgc cggaagttgt tcaataccta acggtaacaa
     4261 atgcgcgcgc tgatcattac ccagtagttt acgcaccgct tcaaactggc acagaaacag
     4321 ttgcgtaggg tcgatcccca ccgcgaggtg cgcgcctgcg ccaatcatgc gccacatgtg
     4381 ataaccgctg ccgcagccga catccagaat ggtgcgtccg gttaaatcag aaagatgggg
     4441 cagaacacga tcccatttcc agtcggaacg ccattcggta tcgatgttga cgccatacag
     4501 tgagaacggc cctttgcgcc acggcatcag gttgcgcatc agcgtttcaa tgcgtttaat
     4561 ttgccctgcg ctcagtggct cttcgctttc tgccgtaacg ctatgcaata aatcaagacg
     4621 atacggttta atttcaggta gaaactccac cgcatttgac cactgcttaa acaacccgtg
     4681 ctgctgctcg cgctgccagt tagcaatctg cgcgggcagc gtttcgagcc agtgtgaaag
     4741 atgatttttg gcaatcagag aataaaagtt accaaagtcg atcatgcagc gtcctctgct
     4801 tttaatgcca ccagtgaacc aaagttaaag cactggaacc acagctcgct atgctcaaaa
     4861 ccggctttat gcaggcgtgc tttatgggtt tccacggaat cggtcagcat cacgttttcc
     4921 agcatgctgc gcttctggct gatctccagt tcgctgtaac cgttggcacg tttaaagtcg
     4981 tggtgcatgt tgaacaacag ttcaccaact ttggcatctt cgaaactgaa tttttccgaa
     5041 agcaccagcg cgccgccggg gttcagccct tgataaattt tatccagtaa cgcctggcgc
     5101 tcggaaggtt ccaggaattg cagggtaaaa ttcagcacca ccatcgatgc gttttcaatg
     5161 gcgatatcgc gaatatcacc ttcaatgacg tctactggcg taggggcttt ataggcgtca
     5221 atatgacgac ggcagcgttc aatcatcgcc ggggagttgt cgatggcaat aattttgcaa
     5281 ttatcatgat gaatgttgcg acgcaccgag agcgtcgccg cgcccagaga acaacccaga
     5341 tcgtaaacct gcgtaccagg ttgaacgaag cgctcggcta acataccaat catggaaata
     5401 atattggaat agccgggaac ggaacgctgg atcatatccg ggaagacttc agctacccgt
     5461 tcatcaaagg tccagtcgcc cagtctggcg ataggggcag aaaatagcgt gtcgcggtga
     5521 gacataacgt aaaaatccgg gaaaaagaaa gtggcgtatt gtgcgctaac gcagggagaa
     5581 aaccaactcc cagggcatat accaaagatt cgccagcacc atcagcaaca gcgcacacca
     5641 ggtggcgctc atgccggaac gtcgccagcg gaacagacgg tgatgaaaac cgtaataatg
     5701 catcagacga ccagcaagca aaacgatgcc gcaaatatgc accatccagg tttctgcgcc
     5761 attcatttcc ataaacagca tcagcacaat cgcgatggga atatattcca ccgcgttacc
     5821 atgaatgcga atagcgcttt gcagttcgct aaaaccaccg tcaccatagg caacgcggta
     5881 ctgcattcgc aggcgaacga catcaaaaga gaacttcatt aataacaacg cacttaaaac
     5941 ggcatacagc gcgcttacca tacaaactcc ctttaaaatg gccgatggca cctgtctatg
     6001 ataggtggca aattcagaaa agagaagatt gctgtggaat agccggaact gctccgatct
     6061 ctggaagcgt tttacgtaag tcttcccaca gggtatgtac cagttccggg gcctgggcaa
     6121 tatctggcgt atgtaaaaaa agataaggcg tagtggtctg atgccactgc gctaattttt
     6181 gtaaccagac ctgaaataat tcccggtttt gcgtcatatc atcactaccg ataaaacgga
     6241 tcagcggatt tgtcgccgtc agtacagcat gtaccggaac tttaggtttt tttcgttgag
     6301 cgtcgcgaat agcttcactg tgtggacgtg ctgcatgaac cgggcggctg tctaaaatca
     6361 cctgattaac gccgcgctga tgtaaaccgc gattaagcgt ttgttcctct tcccctttgg
     6421 cgaaaaactg tggatggcgg acttccacac cataattaaa ttcgccaggg agagaatcga
     6481 gaaaatgcca aagcgcaggc agctcccgtg ggccgaatgt ggcaggcagt tgcagccagt
     6541 attgcccaat gcgcggagcc aacggtgaca tgcgggtcaa aaattcagtc actaaatcat
     6601 cgcaatgtcg taatgctgcc tgatgcgaaa tggtcgccgg aaacttaaaa cagaagcgga
     6661 agtcatctgt ggtctgttca cgccagcgca ggacaacctc gggtttcggc agggcgtaaa
     6721 gcgtggtgtt gccctccacg cagttaatgt gacggcaact accactaaaa caactgatat
     6781 ttaaagatat tatttcagag tctggctcta tggggcatgt atgggacact ctccgtcaac
     6841 tgcttattga gtagttctat ttgcccgata ttgttatctt tcatccatgc tccgtaaacg
     6901 ttgaatacca tttgtgcatt tgcatggccc atttggtttg ctatgaagct tggattcgct
     6961 cctgccgata atgcccagca ggcataagta tgcctagact gataggcttt tctgtgccgc
     7021 agtcctgctc gccttaatgc agatgcccag gaatctctga tggaatctgc tttatagtgg
     7081 tgaccgactt gctggcattt ttttaccagt tgcggattaa aaacaaacgt gcattcatgg
     7141 cgtgcggtgc gaccgaactc ccgaagcttg acgtcaattt gatactgagg gccaagtctt
     7201 gttatttcag cctggctttt cagggcatca acagctggtt gaacaagatg aataacacga
     7261 tcagttcccg cttctgtttt tggtagagtg aactccccga gttttgtata attacgacgg
     7321 atagtcattg ttttagcttt taaatcaata tcctcccagg cgagagatat tagctcaccg
     7381 tgacgaatgc ctgtgtatac tgctaaggac cacaggtttt ttgtttgttg atggtgacag
     7441 gcatcaataa aacgaataaa ttcgtcacgt gtgagcggat ctggttctgt tctggatctc
     7501 tttaatggtg tcaggccgtt aaatgggttt gacacgatat agccgttatc agccgcaaat
     7561 tgaaacattc ctgcaattgt cgtcatgtag taattaaccg taactactga cctcccttta
     7621 atcggcgttg tttttccgtt agaaagatta tgatacccgg tcaataaatc tttcctgata
     7681 aacagtaaat cttcctttgt taccgacgaa gcaagacgtt tttcccctat gcgaggaagc
     7741 atgtttctca ccacggattg ataccgactg aatgcattac ttcctatctc aattttctta
     7801 aggtccaacc atttttccga aagtgcctta acggttatct ctctttttcc cagaccaaag
     7861 tgtttcaggt taggggaatt agggaactgc gcggcgtagt cgaaactccc cattctgatt
     7921 gcaaaacaaa cagaagtgcg aagctcacca gcgatcttcc ggtttttggc ggtgtcagga
     7981 acaccgaggt tttctctgac acgtttgcca ttatagtgaa accatatacg gagtgatcct
     8041 ccatggtttt caacgcctgt cgggtatgat gcgttactca taaaacctcc cagacgtcca
     8101 ggagcattaa caggttaacc ggaacttgca tttttggcac ctggttgttt ctggttttcg
     8161 atccatcgca taatttcttc gatgttgtac aggcattcac tgtaatgccc cggatcacct
     8221 tctacagcgt aatggcggta ttcttttccc tgcatccatg actttcttct ggctcgctcg
     8281 atggtgccag gctttagccc tgttgatgca atgaggactc tctccgtaca ccatttgctg
     8341 ggggttatct gatagatgat tgtctgcatg ccaacctcat aaaactttca tccacggcag
     8401 tggcaccaca cttcaaacat tcgcttcaca tcttctgtac aacacggtgt tgtttatgca
     8461 gcgaacaatt tttaccgggt aaccggcact gtcggtatac agttgtccct gattaatcag
     8521 agcgaacatt ttttctcctg ctctctgaat agtgagaact tcagagccgt atgtttgtag
     8581 cgggttcaat gctgataatt tctgctgaga taagcatccc ggcaagccag agttccccgg
     8641 acaggtcttc atcctggcat atcagttcgc caatattaat ggtggccatg atatccgttt
     8701 tccctgtgcg ctcatccagg acttcttcat aaggcagcgt tgcgtacagg ctttcaatag
     8761 cgcaattgat aacatccagt ccggtcagat tgccgccgac agtaacttcg aatgtttcgc
     8821 ggtattccca tagtccgaaa gttaatcgaa cggtttgttt tgccatgcgt ccgcacgacg
     8881 tcagattcgg gtcatagttc attatttgcg gttgagtatt ctggttgttc atctgatttt
     8941 ccattaaccc ggcggtttgc cgggcgtgta agttatttaa tctggataaa tggtgtattg
     9001 gcaccactgg tcatgtattg cggcagtgta ccgttccact tgttgatggc ttccagctcc
     9061 atgacgccag ggttctggcg cagagcttcg ccgcgtaaac gaatggcatc ggcttctgcc
     9121 agggcttttg ttcgaatggc atcagcctgt ccggcagctt ctgcacgcag catgttggcc
     9181 tccgcttcac gttgcttgac ttcctgttcg cgctgcaggg ttttctggtt tgccgtgact
     9241 ttggcgttaa tgctgtcgat aacggttggc gggtattccg gtttacctac ataagagagg
     9301 ctcattacct gaacgccgat gggggtcatc tcttcctgaa tgtctttaag tgcggcatcc
     9361 agtagttcag acttgccgcc gtcgataaat ttgtcggtgg tcattttgct ggccagtcga
     9421 ttgagtgcgt cggcgatctt ctggcgcagg tcagtggcgg taatgtcatc cacgcctttg
     9481 cggtaggtct gaaacaccgt ggtaactttg gatggatcaa ctttgtaggc aacaccgatg
     9541 tgatagccga tggttgtgcc gtcactcatc tggaagctga acggctcatc gtaggtcttc
     9601 atttgtttga aggttgggaa gatgtaaact tcagtattcc agccagtcca gtagcgacca
     9661 acaccgacca cctcaccgac gcctttatcg tcgcccagtt tatttacttt gatgccaaca
     9721 ttaccgggtt caacgcgatc gcagccgaca aggccaatgg tcggcagaac aatggctaaa
     9781 gcaaaaataa tttttttcat tttttatcct tagtgaaaga aagacccttg taaatggcat
     9841 aaatgcaggg cggggtcaga aacgccagtg caaagccaga agtaactgct atcgtatcct
     9901 tcatggatat aaggaacgga acgagtaatc cgtaaatgca tgcgataatt gccagtaaaa
     9961 ttactattgt gaaatacagt ctcattggta tgtggtatcc cgatattttt aactgacaga
    10021 cagcgcaata aagagaataa tgatttctgt tattgtcagt actgtggcaa ggattaaaag
    10081 cagttttact ctgcttaatt cacggttgtt tttcatataa atggttagta aaaaacggaa
    10141 gaattatatt cttcttaata tttaatgtgt cactggcctt ctggcatacc atgagtattc
    10201 aggtcgttaa tcatttcatc cagaaggagt tcaagccctt ctcgccccat gtctgaaaca
    10261 atgaaacctt tatctgggta tgtaatgagc attttctgat aaagaaacag cgctctaccc
    10321 attccttctg cctcgccgta tttttgaatt aaattccatt caatatactg ctgtaaggca
    10381 aaccgaatgg ggccgggata tatcgtcata accccatgct tcccgttata tattacggcg
    10441 cgatctgttg ttccgtgttc attcgggaca tcaattgtgc cgttcttgtc ttcttcttcg
    10501 ttgatgaatg tcgtcacata caaccatcgc cactgggcaa ccttcatctc aaccggaagt
    10561 tttcccagta atcctgcttc gtcagcttgc gcgagacact gaaggatacg taaacctcgc
    10621 acattaggag tatcgaattc tccagcatcc agacggcgta tggcgtcgtg ataatcaata
    10681 gtcatactgc ctgtttgtat accattggct gttgcttcag cctggaattc agcgtattgc
    10741 atgaaattta ttcctcatct tcatctgctg gtgcaataac gtcatatcct gccttttctg
    10801 caataaacag gaatgttgaa agatttctac aaattcatcg tcatgaacat gacgaattaa
    10861 tattactttc ccgtttttga tggtcagcaa tactctggtt tgttcgtgtt ctgctgtttt
    10921 ctgatgcatt attatctccc gtatgcttta cgcagaaaca agcaggcaat atgcatgtga
    10981 tttttcccgt gttgtgcaat aaggcaggct gttttgtgtg atgccttatg ttttataaaa
    11041 gtcataataa aacctcctgt ggattaagtg tgtgataatc cccggcgatt aagccgtaat
    11101 atagtatctg gagatgactt gttctattta actggatagt tctttttcag ctgctgcttc
    11161 agcatagcaa cgtgcaaatt caagaacttc atcacctgtt ctttttattg cgtcgttgtc
    11221 tgacatttgt aatacgataa ctgcgcataa taaattatgg atattgtttg caaaggaatc
    11281 cggtgccagg cataagcctt catacttatc atggatattg ccagtcattg ttgctgctcc
    11341 tttgcttctg attttcccgt taacaaccac atcaggtcac aattaagcgc actggccagt
    11401 ggtactatat gatcagcagg aacttcacag atgccgcatt cccagtcgtt tatggggtca
    11461 ctgtaggtat gaatcatgcg ggcaagatca gattcggtta atcccagctt tgtacgggcg
    11521 cttttgatgc gtgcgccaat actttccgca tggacttcag tcgtatttac acgaatagct
    11581 gactcgctgt ttttttcact tagtagagaa agcggatcgc atcccaggac gtttgccagg
    11641 gggataagca tgctgatggt tggctcgaac tctccactct cccattgcag aataatctct
    11701 tcgtcaagat cgagtagtct ggctagttcg gtagtcgtca agccgcaggc ttcacgtttg
    11761 gtgcgtatgc aggctatctt gttgcctgat cggatagaat gttgttgttt tgtcgatgcg
    11821 atcgctaaag catattcatg agtaaattct agcacatcaa acccaacctc ttttaatcgc
    11881 gtatcatcta ggaggttgaa ggtgcgaaca gcactgagca ggtttgcgat ggttaatgca
    11941 aaagaatcga gatctaaatc atcacataaa accttgttgt aagtattgag gctacatgtt
    12001 tttggtgttg tttgtaaggc ttccatatca tcaccatgca attctaagtt gaagttaatc
    12061 aatatataat tgatggtgag atattatgtg tcaggaaaac gactgtcaat taaaaattga
    12121 taaacttgat ttttttgtga tggatgacca ataatttaat ttaaaatcaa taactaacca
    12181 aatctgttga tattgaatgg tacagatgag attacttttg actggatata gagcagcgcc
    12241 attccctctt tttcgatgct ccatggctga tagttgggat tgtctgataa gaccatgatc
    12301 ttgcttccaa ttttttgaag tcgtttgacg taacattcgc catcaaagca aaatgcataa
    12361 atgccatcgc cgtcaaagta agttactgtt ttatctaaaa acagtaagtc tcccggggag
    12421 attgttgggg ccatgctgtc ccctctggcg ttaccgattt ctatgttctt gaatgctctg
    12481 ttaccgacca ggcggcgggc atattcagga tcaagttcta ttgagcgtac tacatcaatt
    12541 agatctcccc gtacatgagt tccgtcgccg caactgaatt caacatccag tacattgaac
    12601 acaacgctgt cttctcttgt atggtgtttt tcagctgaag agaatgtcga tgaggcatct
    12661 tcacccaaaa accaggattg tggataacca ctaattactg atagctgtgc aagtttatcg
    12721 cttctgggaa acgtctttcc tgtagtccag tattgtactg attgcgcgct tacacccagc
    12781 ttacgggcta gttcagcctg actccagcct ttcgttttca gcatcattgc tatccgattt
    12841 tctgtgcttt ttacattctt catggtgagt tcctgtcgaa gctttataca cataaatatt
    12901 aacttgattt tagtgtattc gatccttttg aaacttgcat gttaatttat tcttgatgta
    12961 ttcttgattt gtaaagttaa tgttggagct gcgctgtgat aaaactgaat gattacgaca
    13021 tgttgcgtaa attgatccct caaaacgcca tagcgagata catcggtgtt acgccacaag
    13081 cggtgaatct gtggttttct aaaaactccg ttccttctcg ttttgtttta cgggtgtgtg
    13141 aattggttga atggaaggtt actccgcatg ggttaagacc tgatctatac ccttatcccg
    13201 aagatggaat tcctgattcg ttacgaaaat caaacggtat taccagagat taaagactga
    13261 ctgtcacaag cagtaattca cgatgaatac gttttggcgt gagttctgaa atgtcatcct
    13321 caaggagcaa tcagatgaac accgcaattt ttaacgataa agcatccatg accagcgttg
    13381 agattgcaga gctggtgggc agccaacaca aagatgttaa acgcagcatt gaaagactca
    13441 tggggaaggg agttatccga agtgcgccaa tggcgaattt cgaaataatc aacaacttag
    13501 gattgaaaag aaatgtagct gcttacgtat tcgaaggtga acaaggcaaa cgcgacagta
    13561 tcattgttgt cgcgcagatt tgccctgaat tcaccgcccg cttggtagac cgctggcgtg
    13621 aactggaaga acagatccgt aagccaatga gcgaaatcga aatggttgcc gcgatggctc
    13681 ttgaagccgt tcgccaacag aaacggatca ctcaggtgga agaaaaagtc atccacgttg
    13741 ctgaaacagt tgagcaaatt aaaaagggca ctattcgtga gggctatgcc ggatatcgcc
    13801 aactgaaagc aaaaaccggt ttgtcagatg ataaatgctg caatctggtg aacgcctatc
    13861 aaattcctac agacacacat gagttcatga cgccggacgg attgttgtca cgtcgcgcaa
    13921 ttgttgctgt ggaaccgttt atggctgctt tttatcgggt tatggaggaa gcagaaccgc
    13981 gagggactcg ctggtatcac ccgaaaatgg ggttatttca ggttattggt tggcagcggt
    14041 gaaaaaaagc cgggagtaac ccggctcact caacatcaat aacggggagc tgtttcgcat
    14101 aaaacggctc cgaaacatcc aagaacagtt ctaaagatat cagcagctat atgatcattt
    14161 caagaccaaa tattgattct gcaatttcgg gacgttacac tgtctccgca ccttataaag
    14221 cgggtgccgg gggtcgcagc ccggaattgc tcgaggcgat atacgacgcg ccagcgtctt
    14281 ttttatcgtc catgctcgcg cacgccagaa ttatggtggg ctgggcaggg gagccgaaag
    14341 gctcgccggt ttccttgagc gccggtactg cgaaccctgt tcagttcacc acccatgagt
    14401 ttcgcagctc cggtggtgga agtttttcac tgctcaagga ggctgccatc atggctacag
    14461 tcccaacttc cccatacctg aaaattgaag ttgtcaacgg caaggccgtt attttctccc
    14521 tgcatgttgc ctgccacttt aagcgcatgc accagaacat cgtcgacaaa atcgagtatc
    14581 tgaactgctc acgcgagttt tttacccgca atttcatacc gggtacttat cacatctacg
    14641 gtgactccct gcgtggttat tacatcaccc ttgatggtct gatgatgctt cagcttgggt
    14701 taagtctacg cacaatgcgg tactacgaga gctgcattga agcattccat gaggcagaaa
    14761 tcagcctcag tcacaccgct ttccgccgta atcaatggga ggtgcgccca tgattcgccg
    14821 cgtcgttaat tccctgtatc accgatacaa ccgttgcccc cgtgtggggc agtggttcac
    14881 caccagcaac ggccacgttc tgcgggtttg cctggtcaat acagaaagcc agatggttgt
    14941 ctgccaggtt cagggacgta ctcataccct gagttatccg ctggtggctt ttcagtccgg
    15001 gaaaatgttt aagcgtctgg gaggtggcta tgcgtccgtc tgatcttctg ctcgattttg
    15061 ggcatccggt tgcttattac cctgggctcg ttaaatacat gggaagtccg cacgctgtta
    15121 ttttctttgg tcagattttt tactggcagg ataaagcaca tgcagcggaa ggcgtacata
    15181 aaacgcgtga agagatacaa cacgaaactg gacttacatt tgaacaacag gctgtagcgc
    15241 gtaagcatct tgtgtccaga ggcattttgg ttgaaaccaa caagcgtctt gagcacaaaa
    15301 tgttctaccg tatagattgt gagcgcctta atgaaattat caatgaaaac aatcagtttt
    15361 cccgaaatgg ggaaacccgt tttcgggaaa ctgtaaaacc caatttcgcg gaggagggaa
    15421 agccttcacc gcggacacgg gaaaccactc gccgcggaga agggaaaacc aatttcgatc
    15481 ttacagagaa tacaacagag attacttcag agaatactac agagagtaaa aacactattg
    15541 gcgcatccgc tgacgcgtct gcaccagcgc gttctgcccg acaggaatat tcaccggaat
    15601 ttgaacaggc ctggcaggaa tatcccaaac gtgctggtgg taattccaag tcagcagcct
    15661 tcaaagcctg gaaagcccga atcagggaag gtgtgacacc agaaaccatg ctcaacggtg
    15721 tgaaacgcta tgctgcctgg gtgcgtgtct caggaaatac cggcacccag ttcgtgaagc
    15781 aggcgtcgac gttctttgga cccgatcgtc acttcgaaga tttctggcaa cagccagcag
    15841 ctcccggagg tgggcgacag cgacaggtcg atgtcctggc tggcctggga gccatgtctg
    15901 acaaattcgg taaatccagt gacaaattga cattctgagg tgacagcgat gatgacgttt
    15961 aacctgcgtg aacaacaaac aagactacag gcgcggatgg atgagttacg ggcagagatt
    16021 gcatttgctc agaagggcga aaaaccatgg ccttatcgtt cctgctggat gcgtgaaggg
    16081 cgcgggtgtt gcgaaaaaca tggcgaatac cacacacata ttctggtgtg gagcgatcgt
    16141 aatggcgagg acagagaaaa aatttcatgc tgccctgact gcttgatagc tgaggccaac
    16201 gatttgacca tggagctgtc gtccctcaag gcggaagaac tgactgataa cgccggaatt
    16261 gctctgcgtt ttcgggactg cgagtttgat aattatctgg aggttaatcc tgacgcagcc
    16321 agaaatcttg cggcctgtcg ccgctatgcg gagaactggc cagatatgct ggagaacggt
    16381 accagtcttg ttatgaccgg cagttgcggt accgggaaaa atcatctggc ggtatcaatg
    16441 gcaaaacaca tcatccgtaa ctatctggcc agtgtggaga tcaccgacgt gatgcgcctt
    16501 acccgtgctg tgaaaaactg ctggcggaat gacagtgaaa aaacagcgga tgacgtcatt
    16561 gagcattatg cgtcactgga tttgctgatc atcgacgaag tcggcgttca gtttggcagt
    16621 gcggctgaaa tggccatttt gcaggaaatt atcaatgccc ggtatgaggg tattttgcca
    16681 actatcttga tcagcaacct ttcaccggaa gaattgtggg cgttcatcag tccccggatt
    16741 gccgacagga tcaccaatgg cgggcgcaac tggttgtcgt ttaactggcc cagctaccgt
    16801 tctcgtatcg gaggtgttgc cgcatgacca gccagaacac cccggcatgg cgtaacgatg
    16861 acctggaagg cgctgtcatt ggcgcgtttt ttctgcgtgg ggctgatccg gaagtgatgg
    16921 atattctggc cacattgtcg gcggacgttt tttctgcacg acagtaccgg gatatttacg
    16981 cgggaatttg cagacaggca cgtgtatccg gcgtcattga ccccgtactg ctgtgcaatg
    17041 agatgccgga acttgccccg gtgattaccg acaccggacg caaaacctgg gtgaagtctt
    17101 cactggagca ctatgtcgca gcgttgcggc gtaatgccgc actgcgcgat gcagaaaaaa
    17161 cactgactga agcattacag aatttacggg atgcgcatac ctgtgaagca gccgaggatg
    17221 ccctgaagga tgcgcagaac atgatggcct cactgtcgac cggaaagggc gtcattcagc
    17281 cggttcacat tgatgatgtc cttccggaag tggtcgaccg tgttgaatgc cgcaatcagg
    17341 gactggagaa atccagggcg ctgatgaccg gtattgatga actggacgca aaaacgggcg
    17401 gtatggagcc aggcgacctg gtattcattg cggcccgtcc ttcgatgggg aaaaccgaac
    17461 ttgcgctgga tatcatcgac aaggtgactg agcaggggca tggcgtgctt ctgttcacca
    17521 tggagatggc gaacattcag attggtgaac gtatggtgtc tgctgccggg ggaatgccgg
    17581 tatcccgtct taagtctgtt gcccgttttg aagatgaaga ctgggcgcgt ttctcacagg
    17641 gcgtgggacg aatgacgggg cgtaatatct ggatggtgga ccaggcaaac ctgaccattg
    17701 atgagatatg tgcaaccacg aggcaccacc ggatgaaaca cccggaaacg gcgctggtgg
    17761 tggtcgatta cctcggcctg attaaaaccc gcagcacggg gcgtcacgac cttgctgtgg
    17821 gggaaatctc aaagggactt aaaagcctgg caaaatccgg cggttttccg ctgattgctc
    17881 tgagccagct ctcccgtggc gtggaatcca gacccaataa acgccccatg aactcggacc
    17941 tgaaaaactc aggggaaata gaggcggatg cagacatcat tctgatgctt tacagggatg
    18001 aggtgtacaa cccggagaca caggcgagag gcatagctga aatcaacatc acgaaacagc
    18061 gtaatggcac gctgggtacc atttaccggc gttttcataa cgggcatttt ctgcctgtgg
    18121 accaggagag tgcccgggtt ctttccactc ccatgacgcc gggcaatccg cgcagataca
    18181 gcaataaccg catgtcgggt agtaaaacgg agcgtttatt ttgaacaaca gaaccaccac
    18241 tgtttcaccg gaacaacttc gtcggcaggc gcaggagatg cttcgttgtg ctgaacagat
    18301 ggaaaaaacg agcgtggcaa aagatacgct ccgcaagcag cttactccgg cgcttcgtga
    18361 tctgctgcag gcaaaacatc gcacacaaaa ggcggtggat gagctggtgg attgcgtggc
    18421 ggaactggaa ggccaggtaa gccagtttga aacgctggtg aaagagttta ctgcgtgatg
    18481 gctgaatttt ttctcctgcg ttcatgcaat accgttcgct gaggtgaccg tgagagcact
    18541 gctgacccct gaaattgccc cgcgtatggg gatcgtcttg ttcagacccg gttcagagct
    18601 gatgcccctg tttatgcagg ggcgtgtcct gctggagcct gagccggaac gttattcatc
    18661 ttttgccagt ggtgccgttc cggcagcatc acaaccgctg gcggatgatc ctgccgttcg
    18721 ggccgtgttc cgccatgagg cggtgatccg tcgtgctggt ggcgtggaat gccttgagag
    18781 ctggttactt cgtgaaaaag gctgccagtg gcctcattcc ggatggcaca gcgagaacat
    18841 gaccacaatg cgacacgcgc cgggcgcaat ccgtctgtgc tggcactgcg ataacctgct
    18901 tcgcgatcag ttcacggaac gtctggagtc aatggcaacg gataactgtg cccgctgggt
    18961 gttgtccgta gtccgtcgtg atctcggttt tgatgacagt cacgttgtga caatgcctga
    19021 actgtgctgg tggctggttc gtaatgacct ggcggatgcc ttaccggaaa gtgcagcccg
    19081 taaggccctg agattaccga agcctgtttt gccgtctgtc acccgggaga gtgaccttgt
    19141 gccttcggtt cctgccacca gcattatcca ggataaagcg aaaaaggtgc tggcgctgaa
    19201 agtggatccg gagtcgccgg agtcttttat gttacgcccc aaacgtcgtc gctgggttaa
    19261 tgaaaagtac acgcgctggg ttaagacaca gccttgcgcg tgttgtggta agccagccga
    19321 cgatccacat cacctgattg gccatggtca gggtggaatg ggtacaaaag cgcatgacct
    19381 ttttgtgttg cctttgtgca gaaaacacca tgacgaactg catgcggata ccgtggcatt
    19441 tgaagagaag tatggttccc aactggagct gatatttcgt tttatcgatc gcgcactggc
    19501 gattggtgtg ctgtcctgat ttttgtggag aaagttgatg cgtgatattc agatggttct
    19561 tgaacgctgg ggggcatggg cggcaagtgg taacaccggg gtggactatt ctccgatcgc
    19621 tgccggattc aaaggccttt taccatctac cactaaacca cgtccagcct gctgcgatga
    19681 tgacggactt atcattgaaa actgtcttgc acgtctgaag cagaaaaaac ctgaggagta
    19741 ttcgcttctc attgctcatt atttgttgcg aatatcaaaa agacagatag ccaggacgag
    19801 aaagaaaagc gaaaaagcaa tacgaattga gatgcagata gccgaagggt ttattgacgg
    19861 atgtttgtct gtgctggggg taagactgga gatggacgac tggctgctaa aaaagtaaaa
    19921 aatgattagt gcggtccgca aaaagtatgt cagtatgtta agagtggtta ctttgccaca
    19981 cagcttaaat ccgccgtcag gcgggttttt tatgcctgaa atcggaccag tacgttaaac
    20041 gcgctggtgg cggtgaatac cggtctttca gcttgctggc tttttcgaca agagttattg
    20101 gtgtgtcacg ttaaccggaa aagggaaaaa gacatgctga aacagcagga tatgacagaa
    20161 accgccagag tggtgtttaa tgaattaagc gttaccgacc cggcgacagt cggggagatt
    20221 gcgcagaata cttacctttc acgcgaacgc tgccagttaa tactgaccca gctggttatg
    20281 gcgggtctgg cagactatca gtttggttgt tacagacgcc ttcagtcctg aaggcttttt
    20341 tatttgtggt aaatgggcgg ctggtgggtg taaggggcac ccaccagcca tctgctcatg
    20401 cgttgggttc acaagcaaac ctcaggccca ctgctttgcg caaaagcaga atgagcctat
    20461 cagagacagg cttaatgatc catgcttaat actgtaaaaa tatccagttg tgagttaatc
    20521 aacgccgact gcctggaatt tatgcggtcg ttacccgaaa attctgttga cctgatagtc
    20581 acggacccgc cgtacttcaa agtgaaaccc gagggctggg ataaccagtg ggcgggtgat
    20641 gaagattacc tgaagtggct ggaccagtgt cttgcgcagt tctggcgggt gctgaaacct
    20701 gccggaagtc tttacctgtt ctgtggccat cgtctggcat ctgacaccga aatcatgatg
    20761 cgtgagcggt ttaacgtgct gaaccatatc atctgggcaa agccgtccgg acgctggaac
    20821 gggtgcaaca aggaaagcct gcgggcgtat ttccccgcca cagagcgcat tctgttcgca
    20881 gagcattatc aggggccgta tcgtccgaaa gatgccgggt atgaggcgaa gggtaggaca
    20941 ctgaaacagc atgtgatggc cccgctgatt gcttactttc gtgatgcgcg cgctgtcctg
    21001 gggataacgg caaaacagat tgcagatgcc acaggaaaga aaaacatggt gtcgcactgg
    21061 ttcagtgccg gtcagtggca gctgccgaac gaaagcgatt atctgaaatt acaggcactg
    21121 tttgcccggg tggcagaaga gaagcatcag cggggtgaac tggaaaagcc ccaccaccag
    21181 ctggtggata cgtatgcctc tctgaaccga cagtatgcgg agctgcagag tgaatataag
    21241 catctgcggc ggtatttcgg tgtgacggtg caggtgccgt acaccgatgt gtggacgtat
    21301 aaaccggtgc agtactatcc agggaaacat ccgtgcgaaa aaccggcaga aatgttgcag
    21361 cagataatca gcgcaagcag tcgtccggga gacctggttg cagatttctt catggggtcg
    21421 gggtcgacag tgaaagcagc gatggcgctg ggacgtcgtg caactggcgt tgaactggag
    21481 actgaacgtt ttgagcagac ggtgcgggaa gtacaggatt taatcattcg taacggatga
    21541 gattgcggag ttaatcatgc gtcgttatta ttcagcaatc ggccctttag ctcagcggtg
    21601 agagcgagcg actcataatc gccaggtcgc tggttcaaat ccagcaaggg ccaccaaccg
    21661 ccactagctc atcaggaaag aacgtcaccc tgtgcgagat tcggagtccc cggtggcggt
    21721 ccattatcgg tattctgcgt tgttagctca gccggacaga gcaattgcct tctaagcaat
    21781 cggtcactgg ttcgaatcca gtacaacgca ccacaccaca cttatctgcc ctgactctct
    21841 tttgcgggct ttttattaca ggaaagacac cggacagtga aatgttaaat gcctcacaat
    21901 tcaggcagtt gactgttgcc tgacatgctg agcgtttgtt aaaaaaatcc tgcatgatga
    21961 atccccctga gcggcggggc ataatgacag atgtttggtt gcgtattgta taggcaagtt
    22021 gcggattctg tctggtcatt gcagaattca ccgggaggca cccggcatca tgctgtatac
    22081 agagattagg catatatcca ggcttctcat cgcaggagcc tttttacatg caaaaaaaag
    22141 cccgagtggg ttcgggcaac agcatgagat acttgcattg tcatttttat cgtgtggatt
    22201 ttaaccaggg tttataaggc tgcgcaactg cgcggccttt ttcgttttgc gggctgcggt
    22261 tctcctcttt tgattctcct tgtggccgga ccgtggcccg caactgttga ggaaaatccc
    22321 ggaaa
//
LOCUS       DAZFDC010000077        22302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFDC010000077 DAZFDC010000000
VERSION     DAZFDC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.077"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            415..695
                     /gene="drpB"
                     /locus_tag="ACHJ48_002990"
                     /pseudo
     CDS             415..695
                     /gene="drpB"
                     /locus_tag="ACHJ48_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
     gene            735..1430
                     /gene="yedJ"
                     /locus_tag="ACHJ48_002991"
     CDS             735..1430
                     /gene="yedJ"
                     /locus_tag="ACHJ48_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO4708498.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1497..2915
                     /gene="dcm"
                     /locus_tag="ACHJ48_002992"
     CDS             1497..2915
                     /gene="dcm"
                     /locus_tag="ACHJ48_002992"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO4708499.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2896..3366
                     /gene="vsr"
                     /locus_tag="ACHJ48_002993"
     CDS             2896..3366
                     /gene="vsr"
                     /locus_tag="ACHJ48_002993"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO4708500.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLKDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3355..4275)
                     /gene="yedA"
                     /locus_tag="ACHJ48_002994"
     CDS             complement(3355..4275)
                     /gene="yedA"
                     /locus_tag="ACHJ48_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO4708501.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4448..5365
                     /gene="yedI"
                     /locus_tag="ACHJ48_002995"
     CDS             4448..5365
                     /gene="yedI"
                     /locus_tag="ACHJ48_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO4708502.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQVH"
     gene            5444..5626
                     /gene="yodC"
                     /locus_tag="ACHJ48_002996"
     CDS             5444..5626
                     /gene="yodC"
                     /locus_tag="ACHJ48_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO4708503.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            5797..7491
                     /gene="dgcQ"
                     /locus_tag="ACHJ48_002997"
     CDS             5797..7491
                     /gene="dgcQ"
                     /locus_tag="ACHJ48_002997"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO4708504.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7488..8303)
                     /gene="yedP"
                     /locus_tag="ACHJ48_002998"
     CDS             complement(7488..8303)
                     /gene="yedP"
                     /locus_tag="ACHJ48_002998"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO4708505.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8601..8828)
                     /gene="yodD"
                     /locus_tag="ACHJ48_002999"
     CDS             complement(8601..8828)
                     /gene="yodD"
                     /locus_tag="ACHJ48_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO4708506.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8991..9179
                     /gene="dsrB"
                     /locus_tag="ACHJ48_003000"
     CDS             8991..9179
                     /gene="dsrB"
                     /locus_tag="ACHJ48_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO4708507.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9223..9846)
                     /gene="rcsA"
                     /locus_tag="ACHJ48_003001"
     CDS             complement(9223..9846)
                     /gene="rcsA"
                     /locus_tag="ACHJ48_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO4708508.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10136..10921)
                     /gene="fliR"
                     /locus_tag="ACHJ48_003002"
     CDS             complement(10136..10921)
                     /gene="fliR"
                     /locus_tag="ACHJ48_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO4708509.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            complement(10929..11198)
                     /gene="fliQ"
                     /locus_tag="ACHJ48_003003"
     CDS             complement(10929..11198)
                     /gene="fliQ"
                     /locus_tag="ACHJ48_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO4708510.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11208..11945)
                     /gene="fliP"
                     /locus_tag="ACHJ48_003004"
     CDS             complement(11208..11945)
                     /gene="fliP"
                     /locus_tag="ACHJ48_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO4708511.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(11945..12310)
                     /gene="fliO"
                     /locus_tag="ACHJ48_003005"
     CDS             complement(11945..12310)
                     /gene="fliO"
                     /locus_tag="ACHJ48_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO4708512.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12313..12726)
                     /gene="fliN"
                     /locus_tag="ACHJ48_003006"
     CDS             complement(12313..12726)
                     /gene="fliN"
                     /locus_tag="ACHJ48_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO4708513.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12723..13727)
                     /gene="fliM"
                     /locus_tag="ACHJ48_003007"
     CDS             complement(12723..13727)
                     /gene="fliM"
                     /locus_tag="ACHJ48_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO4708514.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13732..14196)
                     /gene="fliL"
                     /locus_tag="ACHJ48_003008"
     CDS             complement(13732..14196)
                     /gene="fliL"
                     /locus_tag="ACHJ48_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO4708515.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14301..15428)
                     /gene="fliK"
                     /locus_tag="ACHJ48_003009"
     CDS             complement(14301..15428)
                     /gene="fliK"
                     /locus_tag="ACHJ48_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO4708516.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQANLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMALAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPQVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSSHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15425..15868)
                     /gene="fliJ"
                     /locus_tag="ACHJ48_003010"
     CDS             complement(15425..15868)
                     /gene="fliJ"
                     /locus_tag="ACHJ48_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO4708517.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(15887..17260)
                     /gene="fliI"
                     /locus_tag="ACHJ48_003011"
     CDS             complement(15887..17260)
                     /gene="fliI"
                     /locus_tag="ACHJ48_003011"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO4708518.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(17322..18008)
                     /gene="fliH"
                     /locus_tag="ACHJ48_003012"
     CDS             complement(17322..18008)
                     /gene="fliH"
                     /locus_tag="ACHJ48_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO4708519.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18001..18996)
                     /gene="fliG"
                     /locus_tag="ACHJ48_003013"
     CDS             complement(18001..18996)
                     /gene="fliG"
                     /locus_tag="ACHJ48_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO4708520.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(18989..20647)
                     /gene="fliF"
                     /locus_tag="ACHJ48_003014"
     CDS             complement(18989..20647)
                     /gene="fliF"
                     /locus_tag="ACHJ48_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO4708521.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSWRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            20862..21176
                     /gene="fliE"
                     /locus_tag="ACHJ48_003015"
     CDS             20862..21176
                     /gene="fliE"
                     /locus_tag="ACHJ48_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO4708522.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            complement(21201..21341)
                     /locus_tag="ACHJ48_003016"
                     /pseudo
     CDS             complement(21201..21341)
                     /locus_tag="ACHJ48_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            21525..>22302
                     /gene="espR4"
                     /locus_tag="ACHJ48_003017"
     CDS             21525..>22302
                     /gene="espR4"
                     /locus_tag="ACHJ48_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR4"
                     /protein_id="HFO4708523.1"
                     /translation="MFPLNDLSLKTQSVQLNKITSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNDKFTPVSEDCNLLNMLSEFKLLREQCFRWGNYTLLFENYEAYDKTGSITI
                     EKNQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGGSNDLKE
                     NVDRALVRMFKCVKLHSNELDLSYLFLGSVPPLPDWIEMLSLVYNELDSIQVPESCKE
                     LELDFNNLTEFPQVPDGITLISVNNNLISHIDSFPPKAKKI"
BASE COUNT         5370 a         5643 c         5857 g         5432 t
ORIGIN      
        1 atcggaaaga tggggggtat caatgattgt tgcgctaaac ggctggtgtg atcacgcgca
       61 cattaatgaa ggcataaaaa aaagccggca tcatgccggc ccaaaaaata taaacaagac
      121 gataaaaggc taataacgga agcatcatga cacagaataa taaaaaggta taattgtaat
      181 cagtgctaat atattgattg aatgaattaa taaagccgat ctcaaagata aataaaatcg
      241 ctcaaaacga tttttcctgt attcactgcc gttgcgcaaa aattatctat ttgttcaaaa
      301 aatgagtgag tcttgactgg ctcatccaat gtggaaaaat gtgactttta tcacgtaata
      361 gtactaagtc tgaattttcc gggttatctc aaaatggaat acggttcgac aaagatggaa
      421 gagagactct ctcgcagccc tggcggcaaa ctggctttgt gggcttttta tacatggtgt
      481 ggctatttcg tctgggcaat ggcgcgttac atctgggtga tgagccggat ccccgatgcc
      541 cccgtgtcgg gatttgaaag cgatttaggc tctaccgcgg gaaagtggtt agggcactgg
      601 tcggattttt atttatggct ctggtcgggg cattgttagg cagcattgcc tggtacacac
      661 gacctcgtcc cgcacgtagc agacgctatg aataaaaact gctaaggtgg cgactctttg
      721 gcaacggagc gcgtatggac ttacaacact ggcaggcaca gttcgaaaac tggttgaaaa
      781 accatcatca gcaccaggac gcggcccatg acgtgtgtca ttttcgccgc gtctgggcta
      841 cggcacagaa gctggcggca gatgatgacg tcgatatgct ggtgatttta accgcctgtt
      901 atttccacga tattgtgagc ctggcgaaaa atcatccgca acggcagagt tcttcaatcc
      961 tggcggcaga agagacgcgt cgcctgctcc gtgaagagtt cgtgcaattt ccggcggaga
     1021 aaatcgaggc cgtttgtcat gccattgccg ctcacagttt cagcgcgcaa atcgccccct
     1081 taacaacgga agctaaaatc gtccaggatg cggatcggct ggaagccctg ggggcgattg
     1141 gcctggcgcg cgtgtttgcc gtttcagggg cattgggcgt ggcgctgttt gatgccgaag
     1201 atccgctcgc acagcatcgc ccgcttgatg ataaacgcta cgcgctggat catttccaga
     1261 ctaagttgtt gaagctaccg caaatcatgc aaaccgtaag aggcaagcag ctggcgcagc
     1321 acaacgcgca ttttttagtc gagtttatgg cgaagctcag tgccgaactg gcgggggaga
     1381 atgaaggtgt cgatcacaag gtgatagatg cgttttcacc cgcgggctga gcgcgtggcc
     1441 ctaaatggct gtaattatgt taacctgtcg accatctcag atggccggtt aaatctatgc
     1501 aggaaaatat atcagtaacc gattcataca gcaccgggaa tgccgcacag gcaatgctgg
     1561 agaaactgct gcaaatttat gatgttaaaa cgctggtggc tcagcttaat ggtgtgggtg
     1621 agaatcactg gagcgcggca attttaaaac gtgcgctggc gaatgactcg gcatggcacc
     1681 gtttaagtga gaaagagttc gcccatctgc aaacgttgtt acccaaacca ccggcacatc
     1741 atccgcatta tgcgtttcgc tttatcgatc tatttgccgg aattggcggc atccgtcgcg
     1801 gttttgaatc gattggcgga cagtgcgtgt ttaccagcga atggaacaaa catgcggtac
     1861 gcacttataa agccaatcat tattgcgacc cggcgacgca tcattttaat gaagatatcc
     1921 gcgacatcac cctcagccat aaagaaggcg tgagtgatga ggctgcggcg gaacatattc
     1981 gtcaacacat tcctgaacac gatgttttac tggccggttt cccttgtcag ccattttcgc
     2041 tggctggcgt atcgaaaaag aactcgctcg ggcgggcgca cggttttgcc tgcgatactc
     2101 agggcacgct gttctttgat gtggtgcgca ttatcgacgc gcgtcgtccg gcgatgtttg
     2161 tgctcgaaaa cgtcaaaaac ctgaaaagcc acgaccaggg taaaacgttc cgcatcatca
     2221 tgcagacgct ggacgaactg ggctatgacg tggctgatgc agaagataac gggccggacg
     2281 atccgaaaat catcgatggc aaacattttc tgccgcagca ccgtgaacgc atcgtgctgg
     2341 tgggttttcg tcgcgatctt aatctgaaag ccgattttac tctgcgtgat atcagcgaat
     2401 gtttccctgc acagcgagtg acgctggcgc agctgttgga cccgatggtc gaggcgaaat
     2461 atatcctgac gccggtgctg tggaagtacc tctatcgata tgcgaaaaaa catcaggcgc
     2521 gcggaaacgg cttcggttat ggaatggttt atccgaacaa tccgcaaagc gtcacccgta
     2581 cgctgtctgc gcgttattac aaagatggcg cggaaatttt aatcgatcgc ggctgggata
     2641 tggccacggg tgagaaagac tttgacgatc cgctgaatca gcaacatcgt ccacgtcggt
     2701 taacgcctcg ggaatgcgcg cgcttaatgg gttttgaagc gccgggagaa gcgaaattcc
     2761 gcattccggt ttcggacact caggcctatc gccagttcgg taactcggtg gtcgtgccgg
     2821 tctttgccgc ggtggcaaaa ctgcttgagc caaaaatcaa acaggcggtg gcgttgcgtc
     2881 agcaagaggc acaacatggc cgacgttcac gataaggcca ctcgcagcaa aaatatgcgc
     2941 gcgattgcca cgcgtgatac ggcgattgag aagcgcctcg ccagtctgtt aaccgggcag
     3001 ggcctggcgt ttcgcgttca ggacgccagt ctgcccggac gcccggattt tgtcgttgat
     3061 gaatatcgct gcgtgatatt tacccatggc tgcttctggc atcatcatca ctgctatctg
     3121 tttaaagtgc ctgcgactcg aaccgagttc tggctggaga agataggtaa aaatgttgag
     3181 cgcgatcgcc gcgatatcag tcgcttgcag gaactcggct ggcgcgtatt gattgtctgg
     3241 gagtgcgcgt tacgtgggcg cgagaagctg aaggatgaag cgcttaccga gcgtctggaa
     3301 gagtggatct gcggcgaagg agccagcgcg cagatcgaca cgcaggggat acatttactc
     3361 gcttgatgcg tcctgaataa ctggagcaac tacgggtttt gccggaaaga gatattttcc
     3421 caacgtgacc agtaccaccg cgaagacaat tacgccgagc gccagccatt caatcttcga
     3481 cagtgtttct ccacccagtc ccgtacccag caagaccgcg accaccgggt taacgtaagc
     3541 gtagctggtg gcgagagccg gactgacatt acggattaaa tacatataag cgttgatggc
     3601 gataatcgaa ccaaacagcg ccagatagcc gaccgcaagg aagcctgaaa gggaagggag
     3661 cgccgtcagt ttttcacccg caatcatcga cgcgatcatt aacaccacgc ctgccgccag
     3721 catctcaatc gcacccgcca tcatccctac aggtaaggta atgcgcgagc cataaactga
     3781 gccaaacgcc cagctaatcg agccgattaa aatcagaatc gcgccccacg gattgccgct
     3841 taaatttcca ccgctattga gcatgatgat tccggcaagc ccaatggcaa tacccaccca
     3901 ttccagtttg cgcgttttaa tgccaaacag gcggctgaag cacagggtaa agaggggcac
     3961 ggttgcaacc actacggcgg cgatgccgga aggaacattt tgatgttcgg caaccgtcac
     4021 catgccatta ccgacagcca gcaataacag gccaatcagc gcggcattga gcagcggacg
     4081 tagcgggggg agtttgtgtc cgcgcagtag taaaaatgcc agcaataaaa taccggctgc
     4141 caggaatcga acacccgcca tcattaacgg aggccagctt tccacgccaa tccgaatgac
     4201 aaaataggtt gagccccaaa tgatatacaa cgcaaacagc gcgccaaaaa gcggtaacaa
     4261 ctggcggaaa cgcataatcc ctcacggtgg aaataaaaag gtggttcata gtaaacgtga
     4321 aaatcattct gctggcgaga gatataattg cacttgattg ttaaaaaaat gttgacctgt
     4381 gaagcaagta tcagagctgc cgtttttgct tcatacttac acccttcaac aataaaaatg
     4441 agagggaatg cttttggccg ggagtagttt actgacgttg ctcgatgata tcgccacact
     4501 gctggacgat atctccgtga tgggcaaact ggcggcgaag aaaaccgccg gtgtattagg
     4561 ggatgactta tcgctcaatg cgcaacaagt ttcaggcgtg cgggccaacc gggaacttcc
     4621 cgtggtctgg ggcgtggcga aaggatcgct gattaataaa gtgattctgg tgccactggc
     4681 gctgatcatc agtgcgttta tcccgtgggc gattacgcca ttgttgatga tcggtggcgc
     4741 gtttctctgc tttgaagggg tagagaaagt gctgcatatg ctggaggcgc gtaaacataa
     4801 agaagatccg gcgcagagcc agcagcgtct ggagaagctg gcggcgcagg atccgctgaa
     4861 gtttgaaaag gacaaaataa aaggggcgat tcgtaccgat tttatattgt ctgcggaaat
     4921 cgtcgccatc acgctgggga ttgtggccga agcgccgttg cttaatcagg tgctggtgct
     4981 ttcaggcatc gcgctggtag tgaccgtggg cgtctacggt ctggtagggg ttatcgttaa
     5041 gattgatgac ctggggtatt ggctggcgga aaaatccagc gcgctgatgc aggcattagg
     5101 taaaggatta ttgattatcg cgccctggct gatgaaagcg ttatcgattg tcggcacgct
     5161 ggcgatgttc ctcgtcggcg gcgggattgt ggtacatggt attgcgccgc tgcatcacgc
     5221 cattgaacat ttcgccgggc agcaaagtgc agtggtggcg atgatattac cgactgtttt
     5281 aaatctgatt cttggattta tcatcggcgg catcgtggtg ctgggagtga aagccgtagc
     5341 gaaaatgcgc ggtcaggtac attaatactc gcacaggcta ttatgcaaaa ttcgccatct
     5401 tcaactaagg tgaacaagtt tcactcgaaa aaaggaggcg agtatgagct ttatggttag
     5461 tgaggaagtt acggttaaag agggcggccc gcggatgatt gtcactggat actccagcgg
     5521 tatggttgag tgtcgttggt atgacggtta cggggtcaag cgggaagctt ttcatgaaac
     5581 cgagcttgtt ccaggggagg ggagtcgttc tgcggaagaa gtttgaaagg gtatcattaa
     5641 gaccaggccg gatcacatcc ggcctggtgt gccaatgcct gcaatgaatg atagtcgcgt
     5701 cttatcatgc caccattata aaattatcgc tgttttgcgg tacgctatcg ccaggttatg
     5761 ccagaatcat aaaaaagcag gtggggagtc gtcagggtgc agcacgagac aaaaatggaa
     5821 aaccagagct ggttgaaaaa actcgcacgc cgcctagggc ctggtcatat cgttaatctc
     5881 tgctttatcg tggtattgct tttttccacc ttgctgacct ggcgtgaagt agtggtgctg
     5941 gaagatgcct atatctccag ccagcgtaat catctggaaa acgtagccaa cgcgctcgat
     6001 aagcatttgc agtataacgt cgacaaactg atctttttgc gtaatggcat gcgcgaagct
     6061 ctcgtagcgc cactggattt cacctctctg cgtgatgctg taaccgagtt cgaacagcat
     6121 cgcgacgagc acgcctggca aatcgaactc aaccgacgac gcaccctgtc agtcaatggc
     6181 gtatcggatg cattagtcag cgaggggaat ctcctgtctc gcgaaaatga aagcctcgac
     6241 aatgaaatta ccgctgcact ggaagttggt tacttgctgc gactggcaca caactcctcg
     6301 tcgatggtag aacaggcgat gtatgtctcg cgtgccggat tttacgtttc gacgcagccg
     6361 accttgttta cgcgcaatgt cccgacgcgt tattacggct atgtcaccca accctggttt
     6421 atcggccatt cacaacgaga aaatcgtcac cgcgcggtac gctggttcac ttcgcaaccg
     6481 gaacacgcca gcaatactga accgcaggtt accgtcagtg ttccggtaga cagtaataac
     6541 tactggtatg gcgtgctggg gatgagtatt cccgtgcgta ctatgcagca atttttaaga
     6601 aacgccatcg ataaaaacct cgatggtgag tatcagctct atgacagtaa gctgagattt
     6661 ttgacctctt ccaatcctga ccatccaaca gggaatattt ttgatcctcg tgaactggcc
     6721 ttgctggcgc aggcaatgga acatgacacg cggggcggca ttcgtatgga cagtcgctat
     6781 gttagctggg aacgtctgga ccatttcgac ggtgtgctgg tgcgcgtcca tacgttaagc
     6841 gaaggcgtgc gcggcgattt cggcagtatc agcattgcat taaccctgct gtgggcgctc
     6901 tttaccacca tgttactcat ctcctggtat gtgattcgcc ggatggtcag caacatgtat
     6961 gttctgcaaa gctcgttgca gtggcaggcg tggcacgaca ccttaacgcg tttatataac
     7021 cgtggcgcac tgttcgaaaa agcccgtccg ctcgcgaaat tgtgtcagac gcaccaacat
     7081 cctttttctg tcattcaggt cgatcttgac cattttaaag cgattaatga ccgctttggt
     7141 catcaggcgg gcgaccgtgt tctttctcat gctgccggat taattagcag ttccttgcgt
     7201 gcgcaggacg ttgccgggcg ggtcggtggt gaggagtttt gtgtgattct gccaggcgcg
     7261 agtctgacgg aggctgcgga agtcgcagaa cgtattcgcc tgaagttaaa tgaaaaagag
     7321 atgttgattg ctaagagtac gacgatacgc atcagtgcct cgttgggggt aagtagcagc
     7381 gaggaaaccg gtgattatga ttttgaacaa ctccagtcac tggccgaccg tcggctttat
     7441 ctcgctaaac aggctgggcg caatcgggta ttcgcgagcg ataacgctta acgggcggag
     7501 aaaaaatggt ccagcccttc acgccatcct tccggtcctt cacgctgcgt tcgccagacg
     7561 cgggccggat cctcatcatg cagatgcacc ccttcacggt ttagcccttt cacaatcacc
     7621 gcgtaatcca ttacctccag taagggcgca tcgtttggcc catcgcccag gccaagtgtg
     7681 gttgggcgtt tgcctgacaa ttgttgatag gtcgcgataa tccagttggc agcctgatct
     7741 tttccggcag aggcatccag gacgtgccag aagcgcgcac cttgcataaa ctgtaagccc
     7801 agttcgttca gacgagcggt aaattgtgcc atacgctcgt cactgtcgcg ccagattagc
     7861 gttaccgacg cctcatgaag ctgcgtcagc gccgcctggc tacggcttaa tcccgtccat
     7921 tcggcgatgg ttgcatcgtc gacatcatca aaagtcgtga atttaaaatg ttctttctcg
     7981 cgtagcgtat ttaaaacctg gctgatttcg ccatggctaa tacctgagat gatgcgtgga
     8041 aaaccgtcta tctcctgcca ttgttcagca agctggatca ctgcgccatt ctctgcaatc
     8101 agcggtaaac cttgtagccc caacattttt tgcaagtaca gcatttccgc tgatgtttta
     8161 ctgctacaga gaatgacggg aacatttgct tcgcgtaaac ggctgagcca gggggctgcc
     8221 ggttgccagt cataactatg actgtccagc agggtgccat caagatcgct aaaaaccagt
     8281 agtggttgtt gaattgaaaa catagtcgcg cagtactccc cttaccagga tgattctcaa
     8341 aacagcacaa attatcaaag atgatttttt cgggagcgat cccgctgacg ccagagccac
     8401 caaggggtga cgtgcgtcac atttctattc ataagtagcg ttaatcattc atatagcgaa
     8461 tattttcttg tcagcgaaaa aaattgcgga taaggtgatg aacacatcag atttcctggt
     8521 gtaacgaatt ttttaagtgc ttcttgctta agcaagtttc atcccgaccc cctcagggtc
     8581 gggatttttt tattgtgcat tcaacgattc acttcagaga cgctgaagtc atgaatatcc
     8641 agttcgaagg catccgccaa ttcacgccat gtatgatatt cacgtccatc gacgctaacg
     8701 tgttcagaat cgttctggct gcgatgaact tcgctttcgc taatttcatt gatcgccgcc
     8761 agcagggcat cgacatcgac gctgacctca cgttttgcgg tatcgctgta ctcttttgcg
     8821 gttttcatca tctttcctcg caaccgttac tctgttgttg ccgccagtcg ttaagtccgc
     8881 ctggcatctc ttaagtatag accgtcgaga aaatcagcac tcgcgcgcgc ctggcgctgc
     8941 cagaaaaacg aaattgttct acactggcac aaagccacag gaggaaaacg atgaaggtga
     9001 atgatcgggt aacagtcaaa acggatggcg gtccgcgtcg tcctggcgtg gtactggcag
     9061 ttgaggagtt tagtgaaggc acaatgtacc tggtttcgct ggaagactac ccgctcggca
     9121 tctggttctt taatgaagca gggcatcagg acggtatctt tgtggagaaa gcagagtaat
     9181 ctaattcagc ctgactggtg ggaaaccacc agtcagaatg tgttagcgca tgttgacaaa
     9241 aataccatta gtcacattat ccgtcagtcg gacgacatgg tagataacct gtttattatg
     9301 cgttttgatc ttacgtttaa tattaccttt atgcgatgaa acggtcttgg ctttgatatt
     9361 catttggtcg gagatttgaa tggttccctg acctgccatc cacattcgca acatactcga
     9421 ttcggttcgg ctcaatgata acgtcggcat atttaaaaac gaggttatcg ttgtctcttt
     9481 tttcagaata tcgccaagga tatcgtcgag agattccggt ttaatcgatt tagaactgat
     9541 caataaattt tttctgacca atagatattc atcaaaatga acattggcaa ttgccataaa
     9601 aacgataaat aacgtattgg gatgttgatt aatgatgagc ttgatacgct gactgttaga
     9661 agcatcgtgg atgaaacagt cctcattaat aaacaccact gaagggcgct gtgaatcaca
     9721 agctatggca aggtcatcaa cggtttcaat gtcgttgatt tctctttttt taacccctct
     9781 actcaacaga tacccggtta aacctagtcg ggtgtaacta cataaatcca taataatcgt
     9841 tgacatggca taccctcact caatgcgtaa cgataattcc ccttacctga atatttcatc
     9901 atgactaaac ggaacaacat gggtcaccta atgcgccact ctcgcgattt ttcaggcgga
     9961 cttactatcc cgtaaagtgt tgtataattt gcctggaatt gtcttaaagt aaagtaaatg
    10021 ttgcgatatg tgagtgagct taaaacaaat atttcgctgc aggagtatcc tggaagatgt
    10081 tcgtaggtat tatttttcgg ataatcctta ggataacatg ataaacgtta cggaattata
    10141 ttaatggcaa ttcactaata atatcagcca gcaaattaaa aatttcactg aataaatgtt
    10201 cgcaaaaagg cgcaattaac ggcattaatg ccgccattaa agagatgccg acagtcagag
    10261 ttaatggaaa tccaataaca aaaatggata attgcggggc catacgatta agtaaaccta
    10321 atgccagatt cagtgtcagc agcagagtaa tgagcggtaa cgccagcatc agcccgttaa
    10381 ggaaaatcaa actccctgct ttggtgagtg ccagaaacgc attgctgttc aacggttcgc
    10441 caccaatcgg cagggtgtga aaggtatcga ccagcagtga aatcaaccat aaatgaccgt
    10501 taaatgtcag gaacagcagt aacgccagca tatccatgat acgcgctaaa acgggcatat
    10561 taagatggct ggccggatcg acaaacgtcg caaatgacag ccccatttgc agaccgataa
    10621 tttcgccagc ggttcgcaca gcggcaaagg caaattgcat ggtaaaacca agcgcaatgc
    10681 cgatcaggat ctgctgcacg gccagccaca gagcaaagaa cgaaaaaaca ggaacatcgt
    10741 tggcaggtaa tgatggggca atggcgaacg tgatcatcat tgccagaccc agttttaccc
    10801 gtttcggtac gctgcgttcg ctcagaatcg gcgcggtgga gatcagcgcc agcacgcgca
    10861 gtaacggcca gaagtacagg cttaaccagg atagccattg ttcgcttgtc acctgcaaca
    10921 tagtacggct acccgatgat atacggcagg ttagtgaaca aggtgcggac gtaatccagc
    10981 aacagattga gcatccacgg tccggcaata ataatggcga taaatacggc gatgattttc
    11041 ggaataaacg acagcgtcat ttcgttaatc tgcgtggcgg cctgcaaaat actgatgata
    11101 aggcccgtga ccaacgctac caacaatagc ggggcagcca gtgccagcgc gactttcatc
    11161 gcttcagtcc ccatcatcat gaccgattca ggtgtcattt tgcctctcta gctgtaaaag
    11221 ctctgcgcca gcgaaccgac cagcaattgc cagccatcca ccagtacaaa cagcatcagt
    11281 ttaaagggca gagcaatggt ggcgggggga accatcatca tccccaatgc catcaacacg
    11341 ctggctatca ccaggtcgat aatcaaaaaa gggatgaaaa tcgtgaagcc tatctgaaat
    11401 gcggttttca actcgctggt cacgtaggcc gggagcaaaa tgcgcatcgg cacggcttca
    11461 ggtccctgca acgggccggt attcgccagt ctggcaaaca accctaaatc tgcctcacgg
    11521 gtctgacgca gcataaactc acgcagcggc tgcgcccctt tttccagcgc ctcctgcatt
    11581 gatattttct cttcgctgaa tggctggtac gcatcgacat aaattttgtc gatcaccggt
    11641 gacataataa aaaaggtcaa aaacagtgcc agccccagca atacctggtt aggtggcgcg
    11701 gagggtgttc ccagcgcgtt acgcaataaa ccaaaaacaa tgatgatgcg ggtgaaactg
    11761 gtcatcatca gtaaaattgc cggaataaac gtcaacgagg tgatgaacac cagcgtctgc
    11821 accgggagcg accagctttg tccaccgcca ggcagcggct ggctggtgat acccggcagt
    11881 tgcgcgaagg cgaggggcgt aatcagccag agaaggacag gtgcgacaga caataaacga
    11941 cgcatcagga tctcccgcta cgcttaagca aatttttcat gaccgactga aaatcggtct
    12001 gcggtatctc ttctgttggt gctgaagggg gaagtttatg cagcagattg atttgccctg
    12061 cggtaacgcc gagcaccagc cgtgcatctt ccacatcgac caccacaacc cgttcacgcg
    12121 cgcccagcga agcactggcg ctaattttca gaccgttaac gccagtgcgt ttcggggcaa
    12181 atcccaaccg ttttaccagc caggcagcag cgaggatcag ggcaataatg gcgatgagtg
    12241 cgccgctcac ctgcagcagt ggcgcagcag aaaccggcgc ggaagattgc acagtagcgt
    12301 ggttattcat cactaacggc tcaggcggcg cattcgctca gacggagtaa tgatatcggt
    12361 gatccgcacg ccatatttat cggcaacgac caccacttcg ccctgggcga ttaaataacc
    12421 gttgatcaga atatccagtg gttcgcccgc cagaccgtcc agcgccacga cggacccttg
    12481 cgtcagacgc aacagctctt tgatggtcat tcgtgtgcgg cccagctcga cggtcagctt
    12541 gaccggaata tccataatca ggtcgatatc ctgcaacgtt ccgctgacat caccaccgcc
    12601 aaattgctgg aacaccgcgt cggcagcgct tttgctgctg gttgattttt gttcgctcaa
    12661 cgcttcagcc cacagatcgt ccattgcgcc gttgttgtca tcggccggat tattcatgtc
    12721 actcatttgg gctgttcctc gttcagagaa tttaaaatcg ggttaatcaa atgttctatc
    12781 cgtaacgcat actgaccgtt gagagtgccg tactgactgg tcagcaccgg gacgccgtca
    12841 acatgggcga tgatgcggtc gggtttttct atcggcagga cgtcaccggg tttcagtttt
    12901 aaaatctgcg acaggcgtag cgagatatcg gcaaagttgg cgaccagctc cagctgtgaa
    12961 tgctgcacct ggcgcaccag gttatcgcgc cagttctgat cttcattacg cgagttttcc
    13021 agcggcgggt taaccaacaa ttcccgtagc ggctcgatca tgctgaatgg caggcagata
    13081 ttaaattcgc cggtcaggtt gccaatctcc acatggaacg gcgtgttaac cacaatgtcg
    13141 ttcggcgagg tggtgatatt ggtaaatttc acctgcattt ccgaacgcac gtactcaact
    13201 tccagcggat taatcgcctt ccatgcgtcg ctatagcctt caagcgccag tttcaacatg
    13261 cggttgatga cgcgctgttc ggtatgggta aactcgcgac cttccacttt ggtcgggaag
    13321 cgtccatcgc cgccaaacag gttatccacg gcgataaaca ccagactcgg tgagaacacc
    13381 accagcccag tgccgcgcag cggttttaga tggataaggt tcaggttggt cggcaccggc
    13441 aggttgcggg caaattcatg gtacggctga atgcggatgg ccccgacggt tatatccggg
    13501 ctacgacgca gcaggttgaa cagccccata cgaaaatggc gggcaaagcg ctcattaatg
    13561 atttccagcg cctgcaaacg ttcgcgcaca acccgtcgtt gggtattcgg atcgtacgga
    13621 cgaatgtcac tttcgccgct aacactggct gtcggttcgt ctttgacttc gctgtcacca
    13681 ttcaacagcg catcaatttc agcttgagaa agaatactat cgcccatgtc gttatcgcag
    13741 aataaaagcg gtatacagca cgtcggtgac atcctgtttc ggttgcccgg caacaagcgg
    13801 ggtggaaagt gtggttttaa tctcggcaat caggtttttc ttgccttctt ctgtcgccag
    13861 tacggcagca tcctgacgcg aaaacagtaa cagcaaacgg ctacggactt ccggcaaata
    13921 ctcactcagc cgcgagcggg tagcttcatc tttcaggcgc agggttatgc cgatataaag
    13981 cacgcgatcc gcatcgccca gattgaccgt aaaggtatcc agcgcataga agaccgggga
    14041 gggcacgacg cgttgctgcg ctttgtcgtc agcagcaacc tgatgcgaat gccagtagct
    14101 gtaacctgcg ctggcacagg ccgcgagggt aatgaatacc agaatcggga tccaaagcga
    14161 tcgcttgctt ttcttgctta tcgcgtaatc agtcatgtgt tgcggtcttc ctgtgtcgct
    14221 actgcttatc ggctaattat cctgcgtctt gagcgcaaca aagcggggaa aagacgcgga
    14281 ttacggtgct acctctgacg ttaggcgaaa atatcaacgc cgctgttgcc tgttacgcgc
    14341 ccttgtaaag agacgggaac cggaagcgta tcgtcgtctt cccccgccag aggttcatgg
    14401 tttactgtgc gttggctttg ctgttgttgg gaagcggcct gctgctgacc actaaagctt
    14461 tcgccactga tgttgctttg ccctaactga atgccacttt cggccagctg cgtacgcagt
    14521 accggcagcg ctgcttccag ggcggcgcgt acatgctgat gcgatgaaac catctggatt
    14581 tgcgcctggt tatcatccac tttgagggag atttgcactt cacctaaatc ctgcgggtgc
    14641 agacgtaact ctgcactttg ttgcccctgg cgggtgaaca gcgaaatatg ctggcttaat
    14701 gattgttgcc attcgtgaga acccagcggt gcactcaaaa caggcgcggc gacggtgggc
    14761 agtggctgtg tctggtgtgg agtgattagc gggctggcgg cagcagtcac cggcgaaggc
    14821 gtgctgatga cttccgcttt actctgggct tctgctacct gcggtgttaa tggctgagct
    14881 ggtgtgccgg gggcatcatc aggctgtgct gttgtgagtt gcgcagaagt cagttttgtg
    14941 aagagcgttg gtttctctgc cggtaacacg gttgacggca catcagtcac tttgggcgta
    15001 ttgtcaaaac ccggcaacat cgcaaaaagg gcgctcaggc ttgcggtgac gtcttcattc
    15061 agatcatccg ctttttcgtc ttttgtcgta tttttgtcag ccaccgcagc caacgccatc
    15121 gtctgagcgg tggttaacgg tgttgatgtg gattgttcgt cgttgatgac aggcggtgtt
    15181 tcatccacag ggatcagtaa attagcttgt tgcgcgtcgg aaacaatatc gctgatcagc
    15241 ggctcgcctt ttgtcgtggg cttatctgtt gccaccagca actggggggc cgctttgtcg
    15301 gtagttgtct cgcctgctaa tgcttcgctc aacaacgcga gaaaatcttg tgcagcatcg
    15361 ctggctttgc cgccaggcaa tgtggtggtg tcaacgtcgg cggtaatcaa gggcgctaag
    15421 cgaatcattc aggtttcctc atggcggcgc gctgggcgaa ctcatccatc tttttctgat
    15481 cgaggcggtt ttctgcaagc agtgccgccg tggattgccg ttcctgcagt gtctgccagg
    15541 cctgcaaacg ttgtttcttt tctcgccagc tgttcagggc aatgtcaact ttctgcgtcc
    15601 actgattaag ttgctggcga tgctgagtaa tggctttttc cagcgtctgg ataaactgct
    15661 gatagttgat ccagcggttg ctggtcatcc cggcactcat atcgctgttg aggttattgc
    15721 gatattcatt ctgataatcg atcagcattt tgagctgttc ttccgcctgc tgacacccgc
    15781 gacgcatttc acccagcagg cgcgcggcat cctctacctc tttttctgcc agatctttca
    15841 gggtcgccag cgcgccatgt tctgccatct gccgttatct cctgggttat gacactgtcg
    15901 ggaaaatacg ctccagcccc tggagagacg cttcccagtc cgcgcgttca aaaatgcctt
    15961 gttgcaaata gccctccagc tgcggccaca gggcgatggc tttgtcgagc atcggatcgc
    16021 tgcctttggc atacgcgccg acgctaacca gatcgcggtt acgctgaaaa ctcgacaaca
    16081 gctgtttgaa ggtgcgcact cgcgcgtaat gttgctcact gatcaacgcc gtcattgcgc
    16141 ggctgatcga cgcttcaata tcgatagccg gatagtgccc ggcttccgcc agtcggcgag
    16201 acagcacaat gtgaccgtcg aggatcgccc gcgcggagtc tgcaatgggg tcctgctggt
    16261 catcgccttc agtaagcacg gtataaaacg cggtaatcga gccgccgccg ctaatgccat
    16321 ttccggcacg ctcgaccagt gccggtaatt tggcaaacac cgacggtgga taacctttgg
    16381 tggcgggtgg ttcgccaatc gccagcgcaa tttcacgctg ggccatcgcg tagcgggtga
    16441 gggagtccat aatcagcaac acatgctgac cacgatcgcg aaaatcttcg gcaatgcgcg
    16501 tggcataggc ggcaccttgc attcgcagga gcggagacac atccgccgga gcggcaatca
    16561 ccactgatcg tgcacgccct tcggcaccga ggatattctc aataaaatct tttacttcgc
    16621 gcccacgttc accaatcaaa cccacgacaa tgacatcggc gcgggtgtaa cgtgccatca
    16681 tccccagcag cacactttta ccaacgccgg acccggcaaa cagccccata cgctgcccac
    16741 gcccaacggt aagcagggca ttgattgggc gcacgccggt gtccagcaca tgttcaatcg
    16801 gtgtacgttg caacgggtta aatggcgggg taatcagcgc accggtttcc gtcgtatcgg
    16861 gggagggcag gccatcgagc ggtttaccgc tgccgtccag aacgcgacct aataacgccg
    16921 gaccgagcgg caactgcttg ccgctttgca gtccttcagc cgaaatgttt ttggcataaa
    16981 cccgcgcgcc gggcaggaca ccttccactt cctccagcgg cattaaaaac agccgttgac
    17041 cgttaaagcc aacgacttcg ctttctactt cgtgcgtttc gctgccgttc tggcgttcaa
    17101 tgacacaggt tgcgccgagc ggcaattgta atccggtggc ttccagcacc agcccggtag
    17161 cgcgggttaa tcgcccgtag cgacgtaccg caggcaactg cgccattttg gcttcaaagt
    17221 tatccagcgt ggttagccag cgagtcaggc gcgtggtcat tacaccactc ctggtgctgc
    17281 cagacggcag agttcttgcc agcgagtcag gcgcgtggtc attacaccac tcctggtgct
    17341 gccagacggc agagttcttg ccagcgagtg gcgacactgg cgtcgagatc gccttcatcg
    17401 gcggagactt tacagccgcc aggatggagg gtgggatcgc cccgcaagcg ccagccatgc
    17461 aaacttaagg tagcgccgag catatcatcc acacgttgca gatcatccgg gtgcacgcgc
    17521 agctgtggtt taccgctgaa taacggttct tgctgcaaca actgttggat ctgtttgatc
    17581 agtgccgagt tatccaccgt tggcgtctga ccgatgacct gacgtgccgc ctccagcgcc
    17641 atctgcatca ggcgcgacgc tatcacacta tcaagtgcat caagggtagt ttgaaattcg
    17701 ctgaccagtt gctgcatccg ggcatgaatt ggcgcttgtt gagacttcgc ctctgccaga
    17761 ccttgctcca gcccctgggc cagtccttcc tgatagccct gcttatgacc ttgctggcga
    17821 ccttcggcaa tacccgcctg ataaccttgc tcatgggcct gcatttgcag ttgcgccagt
    17881 tgctgctcaa ggctgggttc agcctcttca atgatggttt cttccgactc gaccatgggc
    17941 acaaactctg cctgtggtgg cgcgagatcg tccggcgtcc aggttttcca gggcagatta
    18001 tcagacatag gtatcctcgc cgctgccaat taccatctcg ccagtttcgg caaggcggcg
    18061 cacaatcagc agaatcgctt tctgttcgtt ttccacctgc gacagacgca ccggaccacg
    18121 gttggcgaga tcgtcgcgca gaatatcggc ggcacgctgc gacatattgc gcaggaattt
    18181 ctcgcgcagt ggctgctcgg cccctttcag cgcgatcaac agcgattcgg aatccacttc
    18241 ctgcaacaga cgctgaatgc tgcgatcgtc gacatccacc agattttcga acaggaacat
    18301 ctcgtcgatg attttctgcg ccagctcgcc gtcgaattca cgcacggcgg taataacggc
    18361 ttcttcctgc tgagttttca tcaggttgat aatttcggct gccgttctca cgccgcccat
    18421 tttgctgcgc ttgagattct gaccgtcgag caagccattc agtacttcgg tcagttccgc
    18481 cagcgcggct ggctgcacgc cgccaaaggt ggcgatacgc aacatcacgt cgtggcgcag
    18541 acgttcatcg aacaacgcca gaatatcggc ggcctgggcg cgcttcagat gcaccagaat
    18601 ggtggcgata atttgcggat gctcatcacg aatcagatcg gcggcgctct gcggctccat
    18661 aaagttgagc gtttcaatac cgctggcggt atcgcgagtt tcgagaatat cttccagcag
    18721 gctggcggca cgttcctcac ccagagcttt gaccaatacc gaacgcagat aatcgttggc
    18781 gttgatattc agtgcggcaa actgttcagc ttcttgctca aactccgcca gcacatcggt
    18841 tagctgcttg ttggagatct gcgtgacgtt cgccattgca gcgctcaggg tttgcacctc
    18901 gcgctgggag aggtgcttga acacttctgc cgcccggtct tcgccaatgg tcatcagcag
    18961 gatgacgctt ttatcggtgc ctgtcaggtt actcatgatc gttatttatc cactggcgaa
    19021 tgaccagcgc caccacgcgc ggatcgttat cagacatttc acggatacgc tggctcatga
    19081 cttctgcccc cagacgttgg ttagcgcgcc gttgttggag ttgttcgtct ttgctcaggc
    19141 ggacttccac cgcatcttcc acttcctcgc gggcctgcgc ctgttgctgt acagctttca
    19201 tcgcctcagc gcgacgtgtt agctgcgggc gtaccgcttt acgccacagc agccacgcca
    19261 ccagcagtac cagcaaccag cgaccggcag ccagtaactg atcgataaac gcttgctgtt
    19321 gccagaacgg cagttctccg ccgctttcgt cactactatt gaacggcgag ttaacgacat
    19381 tgagcgagtc accgcgtttt tcagaaaagc ccatcgcctc gcgggtcaga tcttcaattt
    19441 gtttcatctg ttcgttgctg agaggcaatg gtttaccgtc tggcaaggtt ttgtaattca
    19501 ccactaccgc gactgacaga cgttgcacat cgcccacgtt cattttggta tgacgaatag
    19561 tgcgatcgac ttcgtagtta ctggtttcat tccgctgtgt gctacgcggc ccactattgc
    19621 tggtggtgct cgcctgctgc tggcggttat tttgatttgc cggaggcgtg ctgattggcg
    19681 cgttattcgc aggagccggt tgattcgaca acgcccccgg tacgccgccc ggataaccgg
    19741 aaccgctttg ctcgctctca ttaagctggc gtgaacgaag cgccgcctga gattcatcac
    19801 cattagggcg atactgttct tccgtttgtt ctttactggc gaagtccagt tgcgccgtga
    19861 cctgggcgtg aatattaccg ttaccaacaa taggcgacag gattgcttca atacgccgct
    19921 gaatacggcc ttcgacatcg ctggcatatt tcaattgagc gtcattaaga tcgcgccagc
    19981 tggtattgga ctgggttaac agatgtccgc cctgatccac cagcgtgacg tttcccggcg
    20041 gcagaccagc aacggcgctg gaaaccagat gcacaatggc gctaatttgc ccttcatcga
    20101 gtgcgcggcc gggtaacaga tttaccgtca ccgatgcaga aggggatttt tgttcacgga
    20161 cgaataaaga cggtttcggc attgccagat gtacgcgcgc cccttttacc gggccgatag
    20221 tttcgatggt acgagaaagc tcgccttcca gcgcccgctg ataattcacc tgttcgctga
    20281 actggctgat accaaacttt tcctgatcga gtagttcgaa accgaccgcg ccgcctttgg
    20341 gcaaaccttg ttgcgccagg cgcaggcgca gctcgtgaac tttatctgcc ggaacttcaa
    20401 tagcgccgct ggcttcgctg aagcggtaag gaatattcat ttgcgtcagt tggctgacaa
    20461 ttgcgccacc atcctgatcg gaaagattgc tgaataatgt gcggtagtcg ggggctttcg
    20521 cccacaggat cagtgcgacc atgaccgcca ctgccgcgga accggcaaca atcaatggaa
    20581 ttttcggatt cgcacgcagg cgattaagcc actcaagaga ttttgtctgg gctgcagtcg
    20641 cattcatcgc gcacctcgtg gctgattatg gacggcgtta ttggcaaagt ggaacaagac
    20701 tcactcccgg gtcagcaaac tcgaaaaatt gacgctccca ttatttgtgc tttcgttttt
    20761 ttctatccgc caataaaccc gtttttttgt tgctatttag cgcctttgtc ttattgactt
    20821 actggtaggc tttgctacca gaaattatcc aggagatgaa aatgtcagcg atacagggga
    20881 ttgaaggggt tatcagccag ttacaggcta cggcgatgag tgcgcgtgcg caggaatcac
    20941 tgccgcaacc gaccattagt tttgccgggc agctgcacgc cgcgctcgat cgcataagcg
    21001 atacacaaac agccgcccgc acgcaggcag aaaaattcac tctcggtgaa cccggcgtgg
    21061 cgttaaacga tgtgatgacc gatatgcaaa aagcctcagt ttctatgcaa atggggattc
    21121 aggtgcgtaa taagctggtg gcggcatatc aggaagtaat gagcatgcag gtataggttg
    21181 tgtaacctgt tgttaatgac atccgatctc accgcgtggg gcatggatgg ggcaaactta
    21241 ctcattatct ggtttgaaat ggatacctga tcctgaaagt tttctgccat ccacgatccg
    21301 tatacacgat aaaccccctg aacatcagag tgtctcattt gacacatcac cacgtcggat
    21361 gttgttacga ccacagacgc aacagtaacc tctggataat tttatctttg ttgatgtttt
    21421 tattcatgtt ccgggctatg gatatgagcg atttttgata gtggtaattt tattgtgttt
    21481 tagaatgcgc cctgggcaat ataatttatt aaacaggagt gtaaatgttt ccattaaatg
    21541 atctgtcgtt aaaaactcag tcggttcaat taaataaaat cacatcgaat actgagtcta
    21601 cgataaaaca acacgagtta gtatctgatg atgcaatcat aaatgaatta tcaagtgagt
    21661 tagtcagttg tcttggaaat gataagttta caccagttag tgaagactgc aacttactga
    21721 atatgctgtc tgaatttaag ttattgagag agcaatgttt caggtggggt aattatactc
    21781 tattgtttga aaattatgag gcttatgata agacggggtc tatcacgata gaaaaaaatc
    21841 agggggaggg gactttaccc attcggcata aattagagtt tatatcgact aatattgcag
    21901 agttgctgga caagttaacg aaaattacag atgccagact ttgcaaaggc ttcagtgact
    21961 gggctagttc agtcaaagaa ggcggatcga atgacctcaa agaaaatgta gatagagcgt
    22021 tggtgagaat gtttaaatgt gttaagcttc acagtaatga acttgactta tcatacctat
    22081 ttttgggttc tgtgccgcct cttccagatt ggattgaaat gcttagcctt gtttataatg
    22141 aacttgattc aatacaggtt cccgaatcat gcaaagaatt agaactcgat ttcaataacc
    22201 ttacagaatt tccacaagta cctgatggaa ttaccctgat ctccgtaaat aataacctga
    22261 tatcgcatat tgactcattt ccgccaaagg ctaagaaaat tt
//
LOCUS       DAZFDC010000078        22041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFDC010000078 DAZFDC010000000
VERSION     DAZFDC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.078"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(341..1240)
                     /locus_tag="ACHJ48_003018"
     CDS             complement(341..1240)
                     /locus_tag="ACHJ48_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4708524.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            1339..2061
                     /locus_tag="ACHJ48_003019"
     CDS             1339..2061
                     /locus_tag="ACHJ48_003019"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4708525.1"
                     /translation="MDKVILITGASSGIGEGIARELGMTGAKVLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQAALDSWGRVDVLINNAGVMPLSPLAAGKQDE
                     WALTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
     gene            2228..2506
                     /locus_tag="ACHJ48_003020"
     CDS             2228..2506
                     /locus_tag="ACHJ48_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708526.1"
                     /translation="MSLYIKLILSIVREISVNTICSLIVVVALSLLSFSSVAKTITAV
                     GSTINSTEKEISLQAEKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(2910..2972)
                     /locus_tag="ACHJ48_003021"
     CDS             complement(2910..2972)
                     /locus_tag="ACHJ48_003021"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708527.1"
                     /translation="MNTVVFVATVEYAYLSLHFY"
     gene            complement(3209..4111)
                     /locus_tag="ACHJ48_003022"
     CDS             complement(3209..4111)
                     /locus_tag="ACHJ48_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4708528.1"
                     /translation="MKGNVSMAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSR
                     SVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGT
                     VRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQ
                     GMVAVRITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDD
                     KKITHQPEGQWAFSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRME
                     GLFLYYPHRNVSPALRMVIDTLKI"
     gene            4357..5415
                     /locus_tag="ACHJ48_003023"
     CDS             4357..5415
                     /locus_tag="ACHJ48_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4708529.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            5557..6685
                     /locus_tag="ACHJ48_003024"
                     /pseudo
     CDS             5557..6685
                     /locus_tag="ACHJ48_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(6864..7820)
                     /gene="paoD"
                     /locus_tag="ACHJ48_003025"
     CDS             complement(6864..7820)
                     /gene="paoD"
                     /locus_tag="ACHJ48_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO4708530.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(7830..10028)
                     /gene="paoC"
                     /locus_tag="ACHJ48_003026"
     CDS             complement(7830..10028)
                     /gene="paoC"
                     /locus_tag="ACHJ48_003026"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO4708531.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLPVITASNAGALSKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFSSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQIDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAILHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            complement(10025..10981)
                     /gene="paoB"
                     /locus_tag="ACHJ48_003027"
     CDS             complement(10025..10981)
                     /gene="paoB"
                     /locus_tag="ACHJ48_003027"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO4708532.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLVAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYTFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            complement(10978..11667)
                     /gene="paoA"
                     /locus_tag="ACHJ48_003028"
     CDS             complement(10978..11667)
                     /gene="paoA"
                     /locus_tag="ACHJ48_003028"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO4708533.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            12085..12699
                     /gene="yagU"
                     /locus_tag="ACHJ48_003029"
     CDS             12085..12699
                     /gene="yagU"
                     /locus_tag="ACHJ48_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO4708534.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(12947..13276)
                     /gene="ykgJ"
                     /locus_tag="ACHJ48_003030"
     CDS             complement(12947..13276)
                     /gene="ykgJ"
                     /locus_tag="ACHJ48_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO4708535.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(13589..14299)
                     /gene="ecpE"
                     /locus_tag="ACHJ48_003031"
     CDS             complement(13589..14299)
                     /gene="ecpE"
                     /locus_tag="ACHJ48_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO4708536.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPEDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            complement(14268..15911)
                     /gene="ecpD"
                     /locus_tag="ACHJ48_003032"
     CDS             complement(14268..15911)
                     /gene="ecpD"
                     /locus_tag="ACHJ48_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO4708537.1"
                     /translation="MRVNLLIAMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDTSGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNQGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            complement(15901..18426)
                     /gene="ecpC"
                     /locus_tag="ACHJ48_003033"
                     /gene_synonym="yagX"
     CDS             complement(15901..18426)
                     /gene="ecpC"
                     /locus_tag="ACHJ48_003033"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO4708538.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGTLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            complement(18452..19120)
                     /gene="ecpB"
                     /locus_tag="ACHJ48_003034"
                     /gene_synonym="yagY"
     CDS             complement(18452..19120)
                     /gene="ecpB"
                     /locus_tag="ACHJ48_003034"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO4708539.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGTLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            complement(19178..19765)
                     /gene="ecpA"
                     /locus_tag="ACHJ48_003035"
                     /gene_synonym="matB"
     CDS             complement(19178..19765)
                     /gene="ecpA"
                     /locus_tag="ACHJ48_003035"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO4708540.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            complement(19840..20382)
                     /gene="ecpR"
                     /locus_tag="ACHJ48_003036"
                     /gene_synonym="matA"
     CDS             complement(19840..20382)
                     /gene="ecpR"
                     /locus_tag="ACHJ48_003036"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO4708541.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            21207..21398
                     /locus_tag="ACHJ48_003037"
     CDS             21207..21398
                     /locus_tag="ACHJ48_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708542.1"
                     /translation="MQAASQREETEWRVQSKRGLMPAYRGEAGQQVNINIMEYSERNV
                     RQLTLNEQEDTSPGKLMLV"
     gene            complement(21468..21608)
                     /gene="ykgO"
                     /locus_tag="ACHJ48_003038"
     CDS             complement(21468..21608)
                     /gene="ykgO"
                     /locus_tag="ACHJ48_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO4708543.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(21608..21871)
                     /gene="ykgM"
                     /locus_tag="ACHJ48_003039"
     CDS             complement(21608..21871)
                     /gene="ykgM"
                     /locus_tag="ACHJ48_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4708544.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT         5126 a         5558 c         5715 g         5642 t
ORIGIN      
        1 aatttcatat ctttttgaat attaatgaaa aaaaacacaa tgagtagtat gaacagtttt
       61 cccccaaaaa aagttttttc tgccttgaga atgcgcggaa agcgaacgcc ttcaccaatg
      121 tgtatagggg tgtgtgtagt ttaaatcttc tattttaatt tatgtattaa aatcattgtg
      181 ttggtggcga ttattggttc ctattatcgc accatttaaa tcaagtagtt accccatatt
      241 taaatacacc acgtttcctc ctgtgccgca tttgtgccat tgtaaccctg gcaattaatc
      301 aaaacactgt tctgacatca ggcaatgcag gtgcagacat ttaagccaat tgctgccgcc
      361 attccttaac gtggtcaatc agagcgcgga gctttggtgc aatattgcga cgctgtggga
      421 aatacagata gaagcccgga aattgtggaa gaaagtcatc aagcagagat acaagcttac
      481 cgctttcaat atacggcctg aaagtttcct gagtggcaat tgttattcct ccgccggcaa
      541 gagccagcct caacatcaga cgcagatcat tagtcgtaat ctgcggttca atcgcaaggt
      601 cgaaagctct cccgttttct tcaaatggcc agcgataagg cgcaacctcc ggggactgac
      661 gccagccgat acacttatga ttcacaagtt cacgcggatg aacaggtgtg ctgttggctg
      721 cgagatagga gggagaggca acgaccattt cacgctgcct gccggtaagg ggcacagcaa
      781 tcatgtcttt ttcaatgacc tctccgagcc ttacgccagc atcgtaacct gcggccacga
      841 tatcgaattc ctcatccgtg acaacaatat caagcgtaac ggctggattg gctgctgcaa
      901 acgaagcgat tagcggacct gaaagaaatt cttcagctat tgacgtcacc gcgattctga
      961 gaagtccgcg tggcacatgg tcagaaacca tctcttcgaa cgctgcctca ataccggata
     1021 aaggtaatga cagcgattta tgcagccgtt ctcccgcttc tgtcaaatta actgagcgcg
     1081 tggtccgcat tacaagcatt gtcccgaatg catcttcaag gcgtcttatt ccctgactga
     1141 ccgctgaacg ggtaacgccc agccgtgcgg cggctttact aaaattactc tcttctgcta
     1201 ccgcgataaa aatgggtaaa agattaagat cgattttcat tgtttaatgc cgctaaccac
     1261 tgagtacatt gaaagcagga tactggaacc aatcataaag ttctattctc tctccgaaca
     1321 catttgaagg agaacgtaat ggataaggtc atattaatta ccggcgcatc aagtggtatt
     1381 ggggaaggta ttgccagaga gcttggaatg acaggtgcaa aggttttact gggagcacgc
     1441 agagttgagc gtatcgaagc cattgcaacc gaaatctgcc gcgcaggagg aattgctaaa
     1501 gcgcgggagt tggatgtcac agaccgacag tccatggccg atttcgtgca agcagcgctg
     1561 gatagctggg ggcgagttga tgttcttatc aataatgcgg gcgttatgcc gctttcaccg
     1621 cttgcagcag gaaaacaaga tgaatgggcg ctcactattg acgtgaatat caaaggtgta
     1681 ctgtggggaa ttggcgctgt acttccggtg atggaagcac agggttccgg tcagataatc
     1741 aatcttggtt ctattggtgc cctttctgtt gtgcccacag gcgcagtcta ttgtgcatca
     1801 aaatttgcag tacgggctat ttctgatggc ttacggcagg aaagctcgaa aatccgtgtg
     1861 acctgcgtaa atcctggcgt tgtcgaaagt gaactggctt caaccattac ccatgtggaa
     1921 accatgaagg caatggacgc ctaccgttct gttgcacttc aacccgcgga tattgcccgt
     1981 gctgtaaggc atattattga atctcccgaa agcgttgata caaccgaaat taccatcaga
     2041 cccactgcat ctgcaaactg aagaataact tttacattca cgttttatta aataggtcag
     2101 aaggaatatt tatatatccc tgtgtttttc atagcaagat acattatgta ttgctcaccc
     2161 cgaaggagat gaaggtttaa ttacagaaag attatttttc acaggaagta aacatgactc
     2221 atgtttaatg tctttatata taaaactcat cctctcaatt gtcagggaaa ttagcgtgaa
     2281 tactatctgt tctttaattg ttgtggttgc actgtcttta ttatcattca gtagcgtcgc
     2341 caaaacgatt actgccgtcg gttcaaccat taacagcact gaaaaagaaa tttctttaca
     2401 ggcagaaaaa caagggaaat catataaaat tctgggcgcg ttttttaaga acagagttta
     2461 tatgatagca aagttaacac cagtcagtaa aaatgatgct tcataaggct catggtataa
     2521 tttttaagac atgatcaaga agcacgtcta ttgactcaaa attgtcattt gtcaggcaat
     2581 ggaatacctg acaaatgacg ctatcccaat ctattgtacc agtcgttctg ctaattctac
     2641 cgatgcttcg gtctggagtg gtagagtaat attggacaca gttttatttt ctctcttttt
     2701 cgagggctgt atatcaacag gaagaataca tccctaagcg gttaatgctg gagtaatcaa
     2761 tagaccagca ttaatcacag atgattacca atcagacaga aatggttaaa tcgtcgaatt
     2821 catatttaat acgttttctt acgccgctga acagcttgaa aaggatgcat attttaaata
     2881 tcagtgaaat gaacagttta ttgccgttat taatagaaat ggagggataa atacgcgtat
     2941 tctactgttg caacaaaaac aacggtattg atcggttttc ataatgaagt aacctgacag
     3001 atatgaaaat gttgattgag gctaaatgcc cggattaagg ttgtcgaggg gagccaggtg
     3061 gttttttctt ttggatgatc tccattgctg gatagtcccc ttgtacagat aagtccgctc
     3121 tttctcgctc aaagcggccc ctggaattga acgcagagtg actacctgac acaatattgc
     3181 acagtgaaga gtcggtcgtt gttaacgttc agattttcaa tgtatcaatg accatccgca
     3241 gagcggggga gacgttgcga tgagggtaat agagaaacaa tccctccatt cgcaggctgt
     3301 aacgctgcaa cactctgata agcttaccgt gttctaaatc atccgcgacc agttcaacag
     3361 gaacataggc caacccgagg cccagccggg cggcttctgc ttccatatag ctgtcggaaa
     3421 aggcccattg tccttcgggt tgatgagtga tttttttatc atcctgattg agttcccact
     3481 ggtatacgct gccgtcggca aactgatagg cgatacaggg atgcgccttt aaatccgccg
     3541 gggtttgcgg aaaaccatag cgacgaaaat gttccggagt ccccacaatt gccatctcca
     3601 tatcgggcgt gatgcgtacc gcgaccatgc cctggctgac ttcaggcccc aggcggacac
     3661 ctgcatcgaa tctttcctcg atgatatcga cgaaccggct ctcatttatc agttcaagct
     3721 ggatatcagg atatcgctgt ttaaataccg ccagctttgg cagcaggcat ttatcaatcg
     3781 cgtgctggct ggcattgata cgcaccgtgc cggagggcgt ctctcgatag tgcgccagcg
     3841 tggcaagccc aagatctaac atttcaaatc cagatgccgt cttctggtag agctgctcgc
     3901 ctgcttgcgt cagcgacagt ttacgcgtgg tgcgcaccag cagctggacg cccagccttt
     3961 cttcaagctc gcgaacagaa cggctgactc ctgactgagc aagacccagc cgcttagccg
     4021 ctgcggtaaa gctgccttcc cgcaccacct gcataaacag gtatagctcg ttgtagttct
     4081 ccctttttgc cattgaaaca ttccccttca tgccggcaat ctcagcatta atttataaca
     4141 atatggtatt aatcttagca ttaatcgccc cctaatcagc actaattctg ctcactataa
     4201 tgaccccatc aaagtaaagc accgcagcgt tgtccgtcgg aaacctgtat ctctctgttg
     4261 ttttttatgg ggatttttat gaaagccttc acccgaacgc taaaaaccgc gatgccggtc
     4321 atgctgcttt tcgcatcatt aagtggagtc acgacaatga gttatgctga ttcaaccaat
     4381 cctaatgccc ctgtgtccat gaccgataaa tgggataaaa cgttcgccga gagccagaaa
     4441 gtcgatcacc gtaaagtctc gttcccgaac cgatatggca tcaccttagt gggcgatctt
     4501 tacctgccta aagaccgtgg cgatcgcaag ctggcggcga ttgcggtcag tgggccattt
     4561 ggcgcggtga aagagcaatc cagcggcttg tatgcgcaga cgctggcgga acgagggttt
     4621 gttaccctgg cgttcgatcc ttcttacacc ggggaaagcg gcggctatcc gcgaaatgtc
     4681 gcctcaccgg atatcaacac tgaagatttc agcgcggcgg tggatttctt agggctgcaa
     4741 aaagaggtag atcgcaaccg tatcgggctg ctcggtattt gtggctgggg cggcatggcg
     4801 ctgaacgacg cggcgatgga tacccgcgtt aaagcggttg ctaccagcgt gatgtatgac
     4861 atgagccggg cgatgggcca tggtgtgggg gatggcgaag accgttattc taccgccgac
     4921 cgtcgtgctg ttctgcaata tttgaatgaa cagcgctgga aggatgcgca gagcggaacg
     4981 ttcgctcacg gtggtcatga tattaatgtc gatagcaacg gcaaggtcag tgcgggcgag
     5041 cgcattctgc cagaaacgct gccggcaaat cctcatccgg tactgaaaga gttctttgac
     5101 tactatcgca tgccgcgcgg cttccacgaa cgttccgtta actctaccgg ggcgtggacg
     5161 gcaacgatgc cgctgtcatt tatgaatatg ccgctgttga gctacgccag tgaaatcacc
     5221 attccgacgc tgattgtgac cggtgagaaa gcacattcac gctattttgc tgaagatgct
     5281 tacaaggcgg ttggcagtaa agacaaagag ttggtggtgg taccaggggc aaatcacgtg
     5341 gacctgtacg ataacgtggc cggaaaaata cctttcgcga agttcgaaca atttttccag
     5401 accaaactga aataaaacct cctctgaatt gatgcaaagc caccggtcta tggccggtgg
     5461 cttttatcct gatctttgtg aaatttcact atgtcgacac taagccaaac accatcacct
     5521 catcatcagc acgcatactg gggcggtatt tttgccatga ccctgtgcgt gtttgtgctg
     5581 attgcttctg aatttatgcc cgtcagcctg ctcacgccga ttgcccgtga cctgggcgtg
     5641 acggaggggc tggcagggcg ggggattgct atctccggtg cgctggcggt tctgaccagc
     5701 ctgacgcttt caacgctagc tggaaagatg aatcgtaagt tcctgctgct ggggatgacg
     5761 gttctgatgg ccgtatcggg gcttattatt gcgctggcta ccagttatct gatgtatatg
     5821 gtcggtcgtg cgatgattgg tgtggccatt ggcggattct ggtcgatgtc agcggcaacg
     5881 gcgattcgtc tggtgccgca gcatcaggtc acgcgtgcgc tggcgatttt caacgccggt
     5941 aatgcgctgg cgacggtcgt ggccgcgccg ctgggaagct atctgggggc tacggtcgga
     6001 tggcgaggcg ctttcttgtg cttggtgccc atggcggttg tcgcctttat ctggcagtgc
     6061 attaagtctg cccagcatgg atgcgaataa aagccacacg tcatgtggcg cggtattccg
     6121 cttatttagc cgccgcatgg ttgtggtcgg catgatagct tgtggcctgt ttttcatggg
     6181 gcagtttgcg ctattcacct atgtgcgccc atttctggag tcggtgacac gggttaattc
     6241 cttaggcttg tcgttgattt tgctgattat cggcgtggcc ggttttattg gcacgctggt
     6301 tgtttcgata tttctcaaca gaaaattcta ccccacgctg atggccatac ccggactgat
     6361 ggccgtcatt gccatcggtc tgatgcttac cgggcatcac gtctggatgg tctcgctgct
     6421 gttaggactc tggggcatgc tggccaccgc cgcgccaacc ggatggtgga catggattgc
     6481 gcgtacgttg cctgacaacg ccgaagccgg aggcgggctc atggtcgcgg tgattcagct
     6541 ctcaatcgcc cttggatcaa cggcaggagg catcgtgttt gaccgccttg gctggcagag
     6601 tacgtttgcc atgagtagtg tgctgctcct ttgcgcgggc gtactgacct ttgttacagc
     6661 acgccagaag gccggtgcgc tttagtccgg cgagggcgcg cgttgctgaa aaagttacac
     6721 aaggcgatga gcaaccacta tgggtagggc gctcttttaa acgatttagg tgattttcta
     6781 aaacggctac tgcaaatttt tctcttaatt tatggtattt cccccggagg tgagaaagcg
     6841 ctttccacgc ccggccgcaa ggatcaggac gacgggggca ggcatgaatc ctcctcctga
     6901 tggagacgta cagaggcgac ttctgccagc acggagaggg ccagtgtatg cgcattccgg
     6961 gctttgggga atatcccgac gggtgcccgg atttgcgctg tctcctccct ggaccatccc
     7021 agctcgtgga gcttttgcag acgtaacgtg tgggttcgat agctgcccaa tgcgccgaga
     7081 taaaagggtt ttgcttctcg cgcggcctgc aacactggca gctcccggtt gagatcatgg
     7141 cacagcaaaa tgaccgccgt atcggtatcg atctgagcgc tggctgaggc cggaaaaaga
     7201 tcgaagatat ggctgtcata gcctgtggct gctgcaagac tcgcggttgc ctgcgcctca
     7261 agagaacgtc cgtaaatcat cagcctgacg catggcctga accccacctc aaagccattg
     7321 agattccagc ccgttcgggt ttgcgtgggc aggcacacca acgattgtgc ttgcggatcg
     7381 tagcgcagcc ccgccggttt tctctgttcc aggcggttca gcacggcgag cagaggctgt
     7441 gccgagcgta gtttatggag cgtcagcgtg atcccaccgc cgcagggcag aacgatgtca
     7501 aaccacggcg aaccttcgcc atagcgaatt tcgcgatcgc agcctgagcc catcatctcc
     7561 agcgcttcaa aagcggcagc ggcctccacg cagccgccag agacaaaacc gcagtaacga
     7621 ccatcttcgc gcaccaccat ctgcgccccg agcgggcgcg ccgcgccgcc gcgtatctcc
     7681 accagcgtca ccagcaccgc tcctttaccg gacattagcg cctctacggc gaagcgcaga
     7741 atggtccggt ggtcatcggt gagaaacgcc tgctctggct tatgccagtg ttcgtcttta
     7801 tcaaaaagcg ggtatgacat tgttcctcct taaaccacat ccggcagctt atcgagcagc
     7861 ttatccagag tgatgggata atcgcgtacc cgaataccgg tggcgttata caccgcgttg
     7921 gcgatagccg cgctcacgcc gcacaggccc agctcaccga cacctttggc cttcatcggg
     7981 gaggatatgg ggtcggtatc atccaggaaa atcacttcct gttttgggat atccgcatgg
     8041 accggcacct cataccccgc catatcgtga ttaacgaagt agcccaaacg gtcatccacc
     8101 gccagctcct ccatcagtgc cgcgcccatg cccatagtca ttgcgccaat gacttggctg
     8161 cgtgcagttt tcggattcag gatgcgtcct gcagcacaca cagcgagcat acgccggacc
     8221 cgaacttctc ccgtcgcgct atgcacgccg acctccacaa aatgcccggc aaaggtcgac
     8281 tgctggtact ccttgctcag tgttccgaat tcaatgctct cttccgctgt cagtctgccg
     8341 cctgcggtgg cctcatgtag tatggcgctt cgggtaccgt tggtaatctt gccgtcggca
     8401 aactgcgact gctcaggatc aaacccgact gccgaggcaa tcatttcgcg aagcttcaca
     8461 caggcggcgt aaacgccgga ggtggaggta ttcgcgcccc attgtccacc agaacccgca
     8521 gaaaccggga aactggaatc gccgaggtga accgcaacct gctccagcgg tacgccaagc
     8581 atttccgctg ccgtctgggc cagaatggtg tagctgccgg tgccaatgtc ggtcatgtcc
     8641 gtttccacgg taacggtgcc gtttggttcg aggtgaaccc gagcccccga tttttccagc
     8701 agattattgc gaaagcccgc cgcgacgccg tggccgacta gccactcccc gtcgcgcacc
     8761 tgtccgggtg tggcgttgcg ctgcttccaa ccaaatttat ccgctccggt gcgcaagcac
     8821 tcgataagct gacggcgaga gaagcggcgc gtcgggtcgg cggggtcaat ctgagtgtca
     8881 ttcaggatgc gaaactcgac gggatcgatg cccgcttttt ccgccagttc gtcgatcgcg
     8941 atttcgagcg ccatcagacc gggggcttcg ccgggcgcac gcatggcgtt cccttccggc
     9001 aaatcaagcg tggcgagccg caggccggta tgacggtttg ccccggcgta gagtaattcg
     9061 ctttgctgta ccgccgtttc cggcgtgccg ccgggcaggt ttccagacca gctttcatgt
     9121 gagatagcgg tgattttccc gctctggtcg gcaccgatac gcaagtgctg aagggtggcg
     9181 gggcggtgcg tggtgttatt gggaatagag gggcggggga gcatcacttt aaccggacgt
     9241 ttcaccgctc gggcggcgag ggccgccagc agcgcatcgc ttctcaggaa cagcttgccg
     9301 ccaaaccctc cgccgatata cggggagata atacgcacat tctccacggg cactttcagc
     9361 gtttttgcca gatcggtgcg gcaccagtca atcatctgat ttgaggtcca gagagtaagc
     9421 ttatttccat cccagacggc catcgaggca tgcggctcca tcgccatatg gctctggtcc
     9481 ggggtcgtgt aggtagcatc aatcttcacc gcagcggagg agaaagcccc gtcaaagtca
     9541 ccgacgttct tgtcgggcgt gtcttccggc ggctgactga cggcctgttt ttcgtccgcc
     9601 agggagtaag ctcctttatt acggcgatag tgcgcctgca ccagcgaggc cgccgctcgc
     9661 gcctgttcga aggtctcggc cactaccaga gcaatggcct gatgatagtg ctcaatagtg
     9721 gggccgccta acagcctggc ggtgtttttg tcgcctttac tgagtgcccc ggcgttactg
     9781 gcggtaatga caggcagtac gcccggcgct ttttgcgcgg cgtccgtatc aagggcggtg
     9841 aggcgtcctt tggcaatggc ggaaccgacg atatagccat aggcggcgtt gggggattct
     9901 tcatgccatt cgtaggcgta gcgtgccgtg ccggtagttt tcagcggtcc gtcgatgcgg
     9961 tcatggggac gaccgacaac cttcagctga tcgatcgggt tttcccctgc gggtttatca
    10021 aatttcatgc ttgtgccctc gcttcagcca gtacggaggc aagcgttcgc ttcgccaaca
    10081 ggagtttaaa ggtgttttca gcggtgggat gggcgctggc gaacagcgcg tcatatacgg
    10141 cctgcgcccc ctgggatagc tgagcatccg cagcctcaat gcgccagggc ttatgtgcta
    10201 ctccgcccag cgcgacgcgc ccgctgccgt caggctgaat aatcgccgcg accgatacca
    10261 gggcaaaggt gtaggaggcg cgatcgcgca ccttacggta gatatgtttt ccgccgagcg
    10321 gcggaggtaa cgtcaccgca acgataagct caccgggaag cagggcggtt tcaatgtgcg
    10381 gcgttttccc cggtgggtga taaaaatcag ccagtgtgat actgcgagtc tttccctccg
    10441 gcgtgatggt ttccaccacc gcatccagca accgcattgc gaccgccata tcgctcggat
    10501 gggtggcaat gcaggcttcg cttacgccta ccaccgcgtg ctgacggcta aagccttcaa
    10561 gcgccgcgca gccgctcccg ggcaggcgct tattgcaggg ctgattggtg tcgtaaaaat
    10621 aggggcagcg cgtgcgctgg agcagattac ctgctgtggt tgcctgattg cgtaactgac
    10681 cagacgcgcc agcgagcagg gcgcgggaga gtaccgcgta atcacgacgc acgcgctcgt
    10741 gagccaccag gtcggtgttc cgtaccagtg cgccgatgcg cagcccaccc gcgtcggtca
    10801 cttcgatctt atcgagcccg agcccgttca catcgataag gtgggtgggc gtttcaattt
    10861 ccagcttcat caggtccagc agattggtcc cgcccgcgat aaattttgcg ccgggtacgc
    10921 gctgagcgct aagtgccgcc tcggcggggg tgttcactcg ttcataggta aacgccttca
    10981 tgattttatc tcccccgcag cgtcttcaat ggcggcaagg atgttagcgt atgcaccaca
    11041 gcgacagatg ttgccgctca tacgttcacg gatctcatcg gcagttcttt ctggagggga
    11101 aaccaaatcg accgtgacgt gactgggaat gccgtcctga atctctttta gcaccgctac
    11161 tgatgagcaa atttgcccgg aggtgcagta gccgcactgg aagccatcat gcttgataaa
    11221 ggccgcctgc atggggtgaa gattatctgg tgaacccagg ccttcaatgg tggtgatctc
    11281 ggccccctga tgcatgactg caagcgtcag gcaggcatta agcctgcgac cattgaccag
    11341 cacggtacag gctccgcact gtccgtgatc gcaacctttc ttggtaccga tcaaatgcag
    11401 attttcacgc aaagcgtcca gtagcgtggt tcgggtatcc acctcaagct gctcggtttt
    11461 gccgttcacc ttaagtgtca ggggcattat ctcaggcgcg ggtgtagctg ccggaacgct
    11521 tgccgcaagc gtagaatgag gataaaccac ggcggccgcc gccgttgcgg cgctcacttt
    11581 aatcagatcg cgacgggtca aacttaaatc gtgcggctca tgcttcccaa cccgattatc
    11641 ttcggggtat tcgccttggt tgctcatacc aggcctccgg tattctaaag gggaaaataa
    11701 gagtgcgttg cgagagtgct gatgcttttt taaacgttaa gcatagtcgg cgacagcagg
    11761 aggattattc ttaaaacgcg aatgcagcta tgagcccggc taataaattc atgttcgccg
    11821 ggatgttgat gatgatggga gctggtttat tgctttagtt gtacgatgca aaaaccgata
    11881 aggaaacctg tgattgtcag ctctgcatca ccctgcaaat ctatgtcact tctaatataa
    11941 aaatagggag aaatgatggt gcttatattc tttggtgatt agacaaccat cttgttatac
    12001 aaaacaatac agttctttac atttaccttg ttttatgaat actcctaaag aggtgtataa
    12061 cataatggta caagcagggt agatatgaat atatttgaac aaactccacc gaaccgcaga
    12121 cgttatggtc ttgctgcatt cattgggctg attgctggcg ttgtttccgc atttgtgaag
    12181 tggggggctg aagttccatt gccgccacgt agcccggtgg atatgtttaa tgcagcgtgt
    12241 ggcccggaat cattaatcag ggctgctggg caaattgatt gctcgcgtaa ttttctcaat
    12301 cctccgtata tttttcttcg agactggctg gggctgacag atcccaatgc ggctgtttat
    12361 acctttgccg ggcatgtctt taactgggtt ggtgttacgc acattatctt ttcgatagtg
    12421 tttgctgtcg gttattgtgt ggtcgcagaa gtatttccaa aaattaaact ctggcagggc
    12481 ttactggcag gtgctttagc ccaacttttt gttcatatga tttcattccc tctcatggga
    12541 ctgacaccac ctctgtttga tctcccgtgg tatgagaatg tttctgaaat ttttggacat
    12601 ttagtctggt tctggtccat tgaaattatt cgcagagatt tacgaaacag aattactcat
    12661 gagccagacc ctgagatccc tttaggctca aacagataat gcattgaatg ataaaaatgg
    12721 cgcaaataca gcgccatttt tataggttaa aaacattgct ttttatattc tgattcagat
    12781 agtcagtgag tatatcgcgc tacttcagga tgatgtagat ccgaagaacg ctacagaaga
    12841 gaggcattgt tgctggcaaa tagaagaagt atcggggttt gttacccctg aaaaacgaag
    12901 ccccgctatt acagctggcg gggcagtgca aataattatt gtatatttat aacggtgtca
    12961 gcccgtattt agcccttgca cgattgcaag cctcattgac gactccgttc tcaccagaca
    13021 tggcgaattc tctgcatgtg gacgatcgat ttttatatat cgtgcagcag gcatttttgc
    13081 ccggggttcc tgcaagggca atacatcggg ggtttttctg attagttccg ctcatgcatc
    13141 ggtgaaaagg ggatatttgt tcagtgagcc tggcgggaat agtgccgcca gcatcgtcgg
    13201 cttcggccca gtagaaagag acgcggaaaa atgcacaaca ggcaccacac gtcatgcatg
    13261 gattcagatt gctcataatt caccagtaca gctataaatc gtaaagagaa ctgcggtacg
    13321 tcgtatgcag ggacgtatcg cggttggctg gtgtactttc gatagtgtga gtattgaatg
    13381 atttccagcc gttaccgatt ttacgtccta attagtgaag aaaccactcg ttagcaggcc
    13441 gcctggtacc tttccgtgtc tcctgaacac tgtggtgtgc atatgagaaa gcctatcgtt
    13501 acaggtgata ttgacgatcg cttccacatc atttaaacga tttttgagtc tctgaggtgg
    13561 aattcttccc tcgaccgatg gataagtatc agtccgccga agggggctta gccggacagg
    13621 aatcgctaat cttaatgaat ttgtcgttat agaccagata gtgattcccc ggctgacgta
    13681 actcaggctg atgaaccacg tcttccggac gtagatacca ggcatcgcct tcctcttcgg
    13741 tcgaatcaca tcctggttta atcagtagct taaaccacgt gttgccggtg ttacttaccg
    13801 ttccggtcac ctgatcgaag gaccatttaa actgaacctg acgtggtcgt actaccagaa
    13861 tggtatccat caccaccacc ggctccgtgc taacctcacc gccggtaggg ctgcgttttg
    13921 tcaggttacg agtggggacc tcgcgaaatg agacccggta gtagcgctcg cggttatcct
    13981 gtggcccatg atagtaaaat ttaaaatact cgctctcacc agcctgcaac gccagctggc
    14041 ggggggcgaa aagcagttca ccatccaccg gtcgggtgcg taattcactg ctgcccgggc
    14101 tatcgatagc actgatggca atacggtata tccgcgcgct tttgttgtta ttcactacac
    14161 gtttgctgac aaagtcagtc tcggacggca gtgaaaaagt cagattgccg accgaaatcg
    14221 cctgtgtcaa aggtgccgcc agcatacaga ccgctgtcag cagggcctta gttaatgtta
    14281 cgccacgtcg cctgaacatg aatttctcct gaagcgctga cttcgccaaa ccagccgtta
    14341 tcatctaccg tacgcagaga aatggcgtta tccattggaa tcgaaaattt gactgtggtc
    14401 ttatccatct gccccacttc gccagagata tcagtccacg gtgtggtcaa ccacagcgca
    14461 tcggtcatat cccgccagct atcatcgcac ccggcatcgt aggtttttgt agctccgttg
    14521 cgggtaataa aggaaagcgt cgccgggaac ggtactttcg ccttaccgtc atcggagctg
    14581 aagacacaat aggagcgccc gccaatcacc tgcgcgggtc cggtcacctt gatcagcact
    14641 tcgtcggcag ctgttttacc gctggtggtt acgatatagc cgaaatcgag tgccgactcg
    14701 ccgctgccga catatccctc ccgcgacggt gtctgagtat attcatcgga gataatactg
    14761 atgctgaaat cacggggttt gataatcagc gtgttggagg tagaaaattc ataccagcca
    14821 gactccggtg atgtggtgtt ctgaaagcga aagttgaata gatctttggt gttgatatcg
    14881 ctgattccct ggttcaccat ctgcttaaag aagttgctcg agaagaaaac atagatcgag
    14941 tctgcgctct tcatctcgtt gaaggtgtag taataggcag tattgctcac cggtttccat
    15001 gaactgccat tcagactgaa ctgcatatcg tacgccccga cggccgaggc taacgacgag
    15061 ttggcgatcg ccgggaatat atggattgag gtgttgctga gtccgtttgt ttgcagggta
    15121 tagttaacca tcttacaact taatcctgaa cggctgccga tggtttgcgt ccggcagtcg
    15181 gcgttccctt cgcccagagt cggtactccg tcgctgttga taaatacttc cgccagcgag
    15241 tgggtattta tcaaccgtag atttgctgct ttcgtatggg tgacgttgcg aacataccag
    15301 ttgccggagg cctgatcctt acagcgtgcg ccgctgctgg cgtcatagtt cacagaggtc
    15361 tggcaggcat tgatggtcat tgtaaagctg ctgccgacgg gcatttgttg cagatactgg
    15421 taaaacgcgt ctgacaacat gccgtgcatc cacttcgcgc cgccgctggt caccgtcgcg
    15481 ccataaaatc cactggtgtc ggtggtttgc ggcaggataa gactggtggt catattacac
    15541 ccagcgtacc agttgatgca gcgcaagcca gttaaaggag atgaaactgg tgaattttcc
    15601 agccacatat caaacttcca gttggtataa aggccggtgt tgtaaccgtt atcaatgtag
    15661 ccgaggcttt gctgatagat ggttcctgaa ccattgtatt ttaaaccggt ccagcggttg
    15721 gcaccggtca ggcgcggatc cagcgccccg ccaggagtga cgaaaaagtt gtcgtctgag
    15781 ttgttttcga caaacacaaa ctcgcgtgcc ggtgcatccg cccaggttgt tttgctcact
    15841 gccgctctga gcgcagttac tggccagatt agcgcaaaga ttatcatcgc tatcagtagg
    15901 ttaactctca ttctcttctc ctgtctgcgt caccgccgcc cacgatgaga ggccgctgca
    15961 gaccacatca ccgacccaga cggcaccgcg cgcctggttg agttccagtg ccacttcgca
    16021 ggttttattg ccactgtagc gaaaatcgat agtggggtat ttcttatcca cgtccatgac
    16081 aaactcgccg ttttcatcgg ttcgggttcg gccgatatgg ttgttaatcc gtgcgttagc
    16141 cagcagtgtg ccgtcttccg cacggatacg accggagacg gtaaccatct gcttcacctc
    16201 tggctcaatg acagcgacat tgcctggata gagagtcaga tgacttttgc gaccgctgac
    16261 gatatcgtaa ctgtcgagtg agtttttgct gttctgtaac tccacctcat atcttccata
    16321 gggagagagc gggagatagt tacgcttgcc gttaagcggg aaaatccgcc cgttgatttt
    16381 ggcgctgatc tgaccgtcgt cctccagccc ggtgttgaat atcaccccag cgttgccatc
    16441 agtccgcccg ctggcagcaa tgtttttacc ctgccagccg acgctgccat tggcggttaa
    16501 attggtattg acgtagccgt ccgccgcgct attgacgttc agcgttccgc tggcgtagcg
    16561 agcgtcgaac tgtgcatacg ccccaccact gagagttttg tcatcaccgg tatcgccgga
    16621 gatggctcgt gacagattgg caccaacagt gcgaatggtc ccttcatcaa actgcttgcg
    16681 tgctgacagg tttgccatgg tgtagccgtt ttgatgagtc atccctgcgc taaaccagtt
    16741 gcccagtggt agcgagagat cgagagcgat atatttccct gtattggcgt tgctgtcgcc
    16801 gttgttatag cgctgaatac cggcccgcag gccaagcgaa ccaaaggtac cgctgtagac
    16861 attttgatag taatctgccg tgtaataatg gctgttgtaa cggcggtcat cattgtagct
    16921 gatgctgaat gtgcccagct tcgaccacag tgagttcagg ttgagtgtgc cgccgatagc
    16981 acggttgtcg gcatcgctac gtcgcaattg attgccaatg cgggtttttt cctgattaac
    17041 ccacagcgaa ctaaagcctc ccggtagagt ggcgctgatg ctgccgatgc tgctccatga
    17101 gctgtcactg gccagcatat tttgcaggtt aacgttgatc gctcccccaa gcggcagcgt
    17161 cagacgggtt tcacccaccg cctgattatc gtatccatat cccgttgccg cccagctaag
    17221 cgtactcagt gagccggagg tcgaggcacc tgccagccaa ctctctttag ctggtcgcgt
    17281 ctttttcccg ttttccgacc agcgatccat atgaaagcta ccgccccata cctgccacgc
    17341 cagtggtgca ccgacgccgc gcccccggct aaacagctta ttgacccgct gggtgcgttt
    17401 gctgatcacg cgaccgttaa cgatcacctc aacttccaca tcgtaaatac cgtatggtag
    17461 accccgggta tccacttcat gattgcccat ggtgaagttc tgaacgctta gtaaccgccc
    17521 atcacgggtg agatgtactt cacccgccgc cggtaaaaag gcgatcactg gcgtggctga
    17581 ctggctgctg tcgaagatgg tggagctggc ctggtttccc caggaaaggc cgtaaatctt
    17641 ccctgctgaa atggcggtca tcggccctaa ggactgcaag ttccaggtgt cgagcgttcc
    17701 accggcaaat cggtgaccgg caaaatcgcg ttcatacatc gctttatata attcactgtc
    17761 ctgttgaccg ctaccgatcc cgtacagcga gccgtcgagc accacatgat gttcgcgcag
    17821 tgcagtaacg ttattcagcg acagatagct ggatgtgttg ctcccgccgt tacgcaactg
    17881 gttgttatag atgccgaagt tatagctcag attactgctg agggtgttaa cactggactg
    17941 cccgatgtct tcgctacgtg agcgtagtac agtgcccagc gcttcgcgct tgaccaccag
    18001 ctgcagcagc agttggcgca agctgagatc cagctgcgcg ttgtcagtca gggggatggt
    18061 aagggcctca ttgaacgggg cgttcgccag agccatcagc tgctgtcgag tttgttcgct
    18121 gacactggcg ttatcttcac tctcttccag ctgtattttc cggatgcgta gctgtccatc
    18181 atccaaccag ataaaagcgc tgccgattcg ctgatcgtcc tggcgaccct ggctaccggc
    18241 gagatgaata tagagcggga cgctcatgcc gtcctgaagc gcctgactga aggcctgcgg
    18301 aataatcacc ccacctattt gctgcgcact tatgtcagca gcgctggcat cgggctgaac
    18361 gaacaaaaag accatgccga aggcaaactg ggctttcagt cctggggaga accgtcgtaa
    18421 aggcattgtc gttatccatc tgcacagcta tctatttcac gggaatgaac ttatcaccct
    18481 gccaaagtgc aacccgtcct ttgttatccg ccgtgtccac gcgggtaaaa cgacgcgact
    18541 tgcccggcat caggtagtaa ttctctttac actctttacc attggcggct ttcaggcagg
    18601 ggccgtaggc gaggatccgc agcgtcgcat ttcctgtatt tgtcagggtg ccgttggcgt
    18661 actgaaagtg gtagtttgcc tggcgggggg cgacgaccag aatggtgccg atgcgggcgg
    18721 aagcagtggc cacagcgctg cggttggcat tatcgcgctg cgcatcactg agggcctgat
    18781 caaaccagac aatgcggtag tagcgctctt tttcatctgc tggtccctta tagaagaagc
    18841 ggatcacttc gctggcttgg gcgggtagca gcaagctggc gggagtgagt agcaactcgt
    18901 ccggcttgtc cattgagata acctgcccgt cgtcaagcgg tgaagagagc cgttcgagac
    18961 ggatattgat aaggcgacca ctgtcggtac tgtttttgat cgttttgctc agcgtgctgc
    19021 tgtcactgtt cataaacgat gatatatcgc cgacatccag cgcctgggcc ggagatattc
    19081 cggataacag cagagcgaga agcagaaggt gctttttcat aacaattccg tccaggaata
    19141 aagctggggg gctacccctg ctggtacatc agagagatta actggtccag gtcgcgtcga
    19201 actgtacgct aacgtcgccg ctccagatgc cttccggtag agtgctgtaa tcggttactg
    19261 cggtggtacc attggtggtg ccgctgatga tggagaaagt gaaaccatcc tgtgcggtgg
    19321 tacgattgct ggcattgtaa ccgtttgcca gcgggctaag gttgccgccc agtacgccgt
    19381 tggcggtatc gatcatcacg gtatcgccag ttttttcgac tgccgcgccg ttataatcca
    19441 cgcccacatt cagtgtggaa cctgaggtat ccaactgggt taatgtgttg gtgataagac
    19501 gtgaggtcag tttaaaggcg gtagccgttg agtcaccctc gatagccacg tcaaatagac
    19561 ctttctgtga gttaaaacct ttaatgcctt cggcatactg gaacgccagg ctaccgagtg
    19621 gcgtcacaac cagcttactg gtggtgtctt ttttggctgt tgctgaccag gtcgctacag
    19681 cctgagctgt tacgtcagca gcctgcgcca cacccatgcc ggtaaacacc gttaccagag
    19741 ctattgccag aacctttttt ttcattgtat ttcttcccga gttgaattga ggacgtgatg
    19801 tcccagtgag tcatttttta aaactaactt gcctggagtt tactgaacca acttatataa
    19861 ttttgagtac agcttggcct ctgcattacg ccgatgggta tacactgtct ttacactaca
    19921 attttcaatt ctggcaatcg atttaggcag cattccctga gccgtcatgc ggataatttc
    19981 catttcccgg tcagtgattt tatctttctt aatatctttt ctcaggaacc tgccattaat
    20041 cacataggcc agttcttttc taatatcacg actcagtcca gcgtatacca cgcccctaat
    20101 attgctgttg taataccaat aatttgctaa ggcttctgac tttctggccg caatcaatac
    20161 gacctgtttc ccctttaccg cggataacca ctcattatca cggtttataa attcagttaa
    20221 agaatccgta ttgagattga taaacacgaa atcttttctg attttctcca gcgatagata
    20281 aattaatctg tcgatatcca caatcagttc agatagtcct ttatagaagt aggcgtcatg
    20341 gggagaccag atgtatttat cagaacggtt ttgacattcc atatggtttt caacctcata
    20401 gtccctgctg taatcatttt gccatgtcac tactttccaa acctgtaatt tttacaggta
    20461 cattcagttt ctttagtaaa tcggaagtaa ataagatacg tagcacatat caatataggt
    20521 taaacgagat taagttttta attatttcga ttaggtttaa atttatgttt ttttcctgat
    20581 gagtgtcaat atgtgaacag aaaatgaatt ccatggttaa gtcaatattt acaaaaaatg
    20641 aaaatattgc ttttttgata agaagaaaaa gacattcagt ttcataaggt tattggcaat
    20701 ataacgttca attcttactt gcattttata ttagccagga actttgccct gaggtgttag
    20761 gaatagtctt aatgtgttgt gtaatggact agtaaaaaaa caagtctaaa tgataagaaa
    20821 tagaatatga aactatcatg agtcataatt aaagatgatt tgccaatcag taaggtgttt
    20881 taatggatta acatgccgca caaaatggtt atgggtgttc atcccaataa gtgatgaaaa
    20941 attaaacagg tgtaagtacg tcatctgtcc ggtaagcccc ctaagagcca gattaagtga
    21001 agttaaacga agactgccag ttgtcaccgg aacgatggcc tctttgccag ggcactgcat
    21061 tcagtcatct gtgcgcgtta ctctaactgg cactcgaacg tcggcgacgg ggttaccggt
    21121 ggatcaggcc gttcctgtgg cgtaaacgtt tttatgtcag taccaacaga tttaccacat
    21181 ttatcatctt aacagtttga gtgtaaatgc aggctgcgtc gcaaagggag gaaactgagt
    21241 ggcgtgttca gtctaaaaga gggttgatgc cagcatacag gggcgaagct ggtcaacaag
    21301 tcaacattaa tataatggaa tattcggaga gaaacgtcag gcagttgacc ttaaatgaac
    21361 aggaagatac atccccagga aaattaatgc tggtgtaata aatacaccaa cattaatccg
    21421 gtctgattat taatcagaca aatatcgtta attcgtcgaa ttttaaatca acgttttttc
    21481 ttacgaccct gaacggcctt aaagcgtgga ttagatttac aaatcacata tagccgtcct
    21541 tttcgcttca caatctgaca gtctggatgg cgttcttttg cggtacgcag agagttaagg
    21601 actttcatca cgcccccttt ttcgtgctaa caaaacgacc aaaacgttgg gtgaatcgtg
    21661 caacatttcc ttctgatgcc actgttctca gcttccctgt atagaacggg tgcgatttcg
    21721 aagagacatc aattgttacg tacggatacg ttacgccatc cagctcaatc tcacggtctg
    21781 ttttgatagt cgagccgatt ttaaagtact catcaacact ggtgtcgtgg aacaccacag
    21841 tacgatactc aggatggata ttgggcttca tcatttttac ctgttatgtt ataacataac
    21901 cacaaagata catgctcatc cggatcattt caacacccaa tccctgtgac cattagtggt
    21961 atgtggaatt tcctcaacgg ggaagtctgg cattgttaac agccattggc gacgctccca
    22021 taaacgcctg gatgagtgat t
//
LOCUS       DAZFDC010000079        21938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFDC010000079 DAZFDC010000000
VERSION     DAZFDC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.079"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            313..519
                     /locus_tag="ACHJ48_003040"
     CDS             313..519
                     /locus_tag="ACHJ48_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO4708545.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNR"
     gene            595..891
                     /locus_tag="ACHJ48_003041"
     CDS             595..891
                     /locus_tag="ACHJ48_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO4708546.1"
                     /translation="MNAYYIQDCLEAQSWARYYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            897..1682
                     /gene="bet"
                     /locus_tag="ACHJ48_003042"
     CDS             897..1682
                     /gene="bet"
                     /locus_tag="ACHJ48_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO4708547.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1679..2356
                     /locus_tag="ACHJ48_003043"
     CDS             1679..2356
                     /locus_tag="ACHJ48_003043"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO4708548.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            2356..2538
                     /locus_tag="ACHJ48_003044"
     CDS             2356..2538
                     /locus_tag="ACHJ48_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO4708549.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            2511..2702
                     /locus_tag="ACHJ48_003045"
     CDS             2511..2702
                     /locus_tag="ACHJ48_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO4708550.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            2779..2994
                     /locus_tag="ACHJ48_003046"
     CDS             2779..2994
                     /locus_tag="ACHJ48_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO4708551.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            3093..3314
                     /locus_tag="ACHJ48_003047"
     CDS             3093..3314
                     /locus_tag="ACHJ48_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO4708552.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            3311..4258
                     /locus_tag="ACHJ48_003048"
     CDS             3311..4258
                     /locus_tag="ACHJ48_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4708553.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EEIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIAVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            4260..4436
                     /locus_tag="ACHJ48_003049"
     CDS             4260..4436
                     /locus_tag="ACHJ48_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708554.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     RNSASCLIGEMFSC"
     gene            4770..5126
                     /locus_tag="ACHJ48_003050"
     CDS             4770..5126
                     /locus_tag="ACHJ48_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708555.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     WECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYA
                     EGWNDCREAMLQGDKS"
     gene            5123..5485
                     /locus_tag="ACHJ48_003051"
     CDS             5123..5485
                     /locus_tag="ACHJ48_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4708556.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPNDKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEP
                     ASKVTHWMPLPEPPQEVK"
     gene            5573..5815
                     /locus_tag="ACHJ48_003052"
     CDS             5573..5815
                     /locus_tag="ACHJ48_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4708557.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            5819..5953
                     /locus_tag="ACHJ48_003053"
     CDS             5819..5953
                     /locus_tag="ACHJ48_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708558.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            5972..6226
                     /gene="xisR"
                     /locus_tag="ACHJ48_003054"
     CDS             5972..6226
                     /gene="xisR"
                     /locus_tag="ACHJ48_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4708559.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            6260..7546
                     /locus_tag="ACHJ48_003055"
     CDS             6260..7546
                     /locus_tag="ACHJ48_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4708560.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            7567..8268
                     /gene="mlrA"
                     /locus_tag="ACHJ48_003056"
     CDS             7567..8268
                     /gene="mlrA"
                     /locus_tag="ACHJ48_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO4708561.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQYQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            8328..8435
                     /gene="yohO"
                     /locus_tag="ACHJ48_003057"
     CDS             8328..8435
                     /gene="yohO"
                     /locus_tag="ACHJ48_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO4708562.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(8416..9147)
                     /gene="yehW"
                     /locus_tag="ACHJ48_003058"
     CDS             complement(8416..9147)
                     /gene="yehW"
                     /locus_tag="ACHJ48_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO4708563.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(9152..10078)
                     /gene="yehX"
                     /locus_tag="ACHJ48_003059"
     CDS             complement(9152..10078)
                     /gene="yehX"
                     /locus_tag="ACHJ48_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO4708564.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            complement(10071..11228)
                     /gene="yehY"
                     /locus_tag="ACHJ48_003060"
     CDS             complement(10071..11228)
                     /gene="yehY"
                     /locus_tag="ACHJ48_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO4708565.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLASALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(11235..12152)
                     /gene="osmF"
                     /locus_tag="ACHJ48_003061"
     CDS             complement(11235..12152)
                     /gene="osmF"
                     /locus_tag="ACHJ48_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO4708566.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLRRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAAQGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(12404..14701)
                     /gene="bglX"
                     /locus_tag="ACHJ48_003062"
     CDS             complement(12404..14701)
                     /gene="bglX"
                     /locus_tag="ACHJ48_003062"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO4708567.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITILQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            14897..16612
                     /gene="dld"
                     /locus_tag="ACHJ48_003063"
     CDS             14897..16612
                     /gene="dld"
                     /locus_tag="ACHJ48_003063"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4708568.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGHG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(16650..17582)
                     /gene="pbpG"
                     /locus_tag="ACHJ48_003064"
     CDS             complement(16650..17582)
                     /gene="pbpG"
                     /locus_tag="ACHJ48_003064"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO4708569.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(17756..18343)
                     /gene="yohC"
                     /locus_tag="ACHJ48_003065"
     CDS             complement(17756..18343)
                     /gene="yohC"
                     /locus_tag="ACHJ48_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO4708570.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            18513..19091
                     /gene="yohD"
                     /locus_tag="ACHJ48_003066"
     CDS             18513..19091
                     /gene="yohD"
                     /locus_tag="ACHJ48_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO4708571.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGLAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(19221..19982)
                     /gene="yohF"
                     /locus_tag="ACHJ48_003067"
     CDS             complement(19221..19982)
                     /gene="yohF"
                     /locus_tag="ACHJ48_003067"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4708572.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKVPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(20035..21471)
                     /gene="mdtQ"
                     /locus_tag="ACHJ48_003068"
     CDS             complement(20035..21471)
                     /gene="mdtQ"
                     /locus_tag="ACHJ48_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO4708573.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            21695..21778
                     /gene="yohP"
                     /locus_tag="ACHJ48_003069"
     CDS             21695..21778
                     /gene="yohP"
                     /locus_tag="ACHJ48_003069"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO4708574.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         5609 a         5655 c         5572 g         5102 t
ORIGIN      
        1 ttgctattga ctacaccaat agccattgct attgccatgc tattgcatcg atgttggtat
       61 cacaaaattt ataacttcac cgttgcgatg accgcttaga tccgcagctt gaatttcagc
      121 aggctccggg gagtgcgagg ggtgaagcgg acgcgtgaac gtcggtgtga ccagctgaaa
      181 tcaactcaac acttcatacc tcagtcgctt caacgaggcg acttagttat gacaaccggc
      241 ggccatccac cgcctgaata cgcgcagaag tctctatatg ttcagcagcc cagcttacgg
      301 gcaggagttt ttatggttca tcaacattac ggaacgcaga ccgttaatcg aggtgcggtc
      361 atgccaggaa tgctggtcaa acacaaagat ggtacctgga ctgcatcagc taatttacgc
      421 ggacggcttt atctgcatcg cggcatcgag cgcacttata cccgtgattt gctcgtggaa
      481 gtttttctcg acggacgcgg taacggcctg aatcgctaat cccctttcct gttttcctaa
      541 tcagcctggc atttcgcggg cgatattttc acagccattt tcaggagttc agccatgaac
      601 gcttattaca ttcaggattg tcttgaggct cagagctggg cgcgttacta ccagcagatc
      661 gcccgtgaag agaaagaggc agaactggca gacgacatgg aaaaaggcct gccccagcac
      721 ctgtttgaat cgctatgcat cgatcatttg caacgccacg gggccagcaa aaaagccatt
      781 acccgtgcgt ttgatgacga tgttgagttt caggagcgca tggcagaaca catccggtac
      841 atggttgaaa ccattgctca ccaccaggtt gatattgatt cagaggtata aaacggatga
      901 gtacagcact cgcaacgctg gcagggaagc tggctgaacg tgtcggcatg gattctgtcg
      961 acccacagga actgatcacc actcttcgcc agacggcatt taaaggtgat gccagcgatg
     1021 cgcagttcat cgcattgttg atcgtcgcca accagtacgg ccttaatccg tggacgaaag
     1081 aaatttacgc cttccctgat aagcagaacg gcatcgttcc ggtggtgggc gttgatggct
     1141 ggtcccgcat catcaacgaa aaccagcagt ttgatggcat ggactttgag caggacaatg
     1201 aatcctgcac atgccggatt taccgcaagg accgtaatca tccgatctgc gttaccgagt
     1261 ggatggatga atgccgccgc gaaccattca aaacccgcga aggcagagaa atcacggggc
     1321 cgtggcagtc gcatcccaaa cggatgttac gtcataaagc catgattcag tgtgcccgtc
     1381 tcgccttcgg atttgctggt atctatgaca aggatgaagc cgagcgcatt gtcgaaaata
     1441 ccgcatacac tgcagaacgt cagccggaac gcgacatcac tccggttaac gatgaaacca
     1501 tgcaggaaat taacactctg ctgatcgccc tggataaaac atgggatgac gacttattgc
     1561 cgctctgttc ccagatattt cgccgcgaca ttcgtgcatc gtcagaactg acacaggccg
     1621 aagcagtaaa agctcttgga ttcctgaaac aaaaagccac tgaacagaag gtggcagcat
     1681 gacaccggac attatcctgc agcgtactgg gatcgacgtg agagctgtcg aacagggaga
     1741 tgatgcgtgg cacaaattac ggctcggcgt catcacagct tcagaaattc acaacgtaat
     1801 agcaaaaccc cgatcaggaa agaagtggcc tgacatgaaa atgtcctact tccacaccct
     1861 gctggctgag gtttgcaccg gtgtggctcc ggaagttaac gctaaggctc tggcctgggg
     1921 aaaacagtac gagaacgacg ccagaaccct ctttgagttc acttccggcg tgaatgttac
     1981 tgaatccccg atcatctatc gcgacgaaag tatgcgcacc gcctgctctc ccgatggttt
     2041 atgcagtgac ggcaacggcc ttgagctgaa atgcccgttt acctcccggg atttcatgaa
     2101 gttccggctc ggtggtttcg aggccataaa atcggcttac atggcccagg tgcagtacag
     2161 catgtgggtg acacgaaaag atgcctggta ctttgccaac tatgacccgc gcatgaagcg
     2221 tgaaggcctg cattatgtcg tgattgagcg gaatgaaaag tacatggcga gttttgacga
     2281 gatggtgccg gagttcatcg aaaaaatgga cgaggcactg gctgaaattg gttttgtata
     2341 tggggagcaa tggtaatgaa gcatcctcac gataatatcc gggtaggcac gatcactttc
     2401 gtctactccg ttacaaagcg aggctgggta tttcccggcc tttctgttat ccgaaatcca
     2461 ctgaaagcac agcggctggc tgaagagata aataataaac gaggggctgt atgcacaaag
     2521 catctcctgt tgaattaaga acgagtatcg ggatggcaca tagcctcgct caaattggag
     2581 tcaggtttgt gccaatacca gtagaaacag acgaagaatt tcatacgtta gccacatccc
     2641 tttcacaaaa gctggaaatg atggcggcga aagcagaagc aaacgagaga gacccggcat
     2701 gacaacaaca gaatgcattt ttctggcagc aggcttcata ttctgtgtgc ttatgcttgc
     2761 cgacatggga cttgttcaat gacacctcag caggaaaacg cccttcgcag cattgcccgt
     2821 caggctaatt ctgaaatcaa aaaagccaga cagcagtttc cggataaaaa cgtcgatgac
     2881 atttgccgta gcgtactgaa gaagcaccgc gaaacggtaa cgctaatggg attcacaccg
     2941 actcatttaa gtctggcgat cggcatgtta aacggcgtct ttaaggagcg atgaacatga
     3001 aaagcaaaat catcagggag ctacaggctc cttttttatt gttcgcattc accctcaagc
     3061 gtattaacca acaattcagg gattaatgga agatggcaga catcattgat tcagcatcag
     3121 aaattgaaga attacagcgc aatacagcaa taaaaatgcg tcgcctgaac taccagactg
     3181 tatccgcaac tcattgttgt gagtgtggcg atccgataga tgaacgaaga cgcctggcag
     3241 ttcagggttg tcggacttgt gcaagttgcc aggaggatct ggagcttatc agtaaacaga
     3301 gaggttcgaa gtgagcgaaa ttaattatca ggcactgcgt gaaaaggcag agaaagcaac
     3361 taaaggaagc tacatcgtag ggcatacatc tgttaaccag cacggcaatt taacaggagt
     3421 ttttgtttgc caaaaatgga aaggagaacc cggtggtgtg attgcggaat gtcatgttaa
     3481 ctgcctggtt gaaacagatg ttcaggctta tgcaaacgct gaatttattg ctgcctttaa
     3541 tccaaatgtt gcgctggcgc ttctggatga acgggaaaga aaccagcaat acatcaaacg
     3601 ccgcgaccag gagaacgagg agattgcgct tacggttggg aagctgcgtg ttgagcttga
     3661 agcagcaaaa tcaaaactca acgagcagcg tgaatattac gagggagtaa tcgcggatgg
     3721 aagtaagcgc atagcagaac tggaaaaaca atgcgccgaa tgggagcgaa aagcattaag
     3781 caactttgaa gagtgtgctg cgatggctga acgtatcgaa gagatgcaga caaaatctgc
     3841 accagattcg tttggcatca tcggtgaaaa tattcgaaca caggacaatc gaataacgtc
     3901 agatcccatg ttttgtgtgt atcaaaagcg cgaaatcgct gttgatgctg attatgacca
     3961 tgaccggatt gtctgggttg acgaagatgg caatgaagcc aataaacgcc atagtcgtcg
     4021 tctcgagcta cttcatgaaa actttcgaga gccaccagaa aaatggcggc gcgttgctgt
     4081 gaaagatatt gatgaattcg ttacctgctg tttcaccgaa cagggttgta aagactacct
     4141 ggcagtcaat ggtcacaatc ttcgcttgcc atttatatat gtaaaaagcg gtttcaggaa
     4201 cgctgaatat atcggcataa gaaactggct tgctggcatt cgcatcaaag gagagtgata
     4261 tggcgttaac acaccgcgaa ctctgtcaga ttgcgtacaa gttccttaag cgcaacggtt
     4321 tcaaggtttg ttttcatgac cgctttatag ctgtaaccag taccggagaa cagccagatg
     4381 ctatgggatt cagaaattca gcatcatgcc tgattggcga aatgttctcg tgctgacttg
     4441 ttggcagata gaaaaaagcg ttttcgtaaa aatccgtctc ttggaatggg cgactggcga
     4501 ttctttatta gtgagccggg aattatttca attgaggatt taccacctgg ctggggatta
     4561 cttcacgttg ttaacggaag agtacggaaa gtacatgggt ggcccaaggg taattgctgt
     4621 tggggtaatc ctgacgataa gccatttact ggaaataagc aggttgaatg cgattacatg
     4681 ttgtctgcat taaggcgcat ggagttgaga gggcacctta atgaaatata tgacggtgta
     4741 attgttaata agaaagaagg aaacgcggca tgaccactat taccgacaaa gaactgatta
     4801 aagaaatcaa agagcgcata ggcagcttgg acgttcgaga caatattgag cgccgtgctt
     4861 atgaaattgc actggcatcg ctggaagcag aaccgatagc gtgggaatgc ggtgaaaaca
     4921 taatcctgtt taaccctgac acagttgaag catacgcaaa acgtgcggaa atatcaccta
     4981 aaccactatt ctccgcgccg ccagcgctgg tagtgcctga taagttgccg cgtgaataca
     5041 gaaacggttg gcctcttgcg tatagtgatt atgctgaagg ctggaacgac tgccgcgaag
     5101 ccatgcttca gggagataaa tcatgattaa tcgtatcaag ctggagcaca tcctcgaata
     5161 tgccaggcag cagaggcata ttggtcagca ttgtaaaatt ccaccaggag atatggttga
     5221 aatcatggag attgccatgc gcaaggctgg caactctccg gtaactccgg atggttggat
     5281 aagctgtagt gagcgaatgc cgaacgataa acagtatgtt tggtgttggg ggaagtctta
     5341 cggctggact gagtgcgata ccttcgaagg gtattacgat tgttcgagaa acaaatggtg
     5401 ggcagttact gacaatgggg aagaaccggc atcgaaagta acccactgga tgccgctacc
     5461 ggagccgccg caggaggtga aatgatgaat tggcctgaag cattcaccgc agttggagtt
     5521 gcaatagcgg tggcatttat tctgtattcg cttttccgct ggggataaag gaatgttcgc
     5581 tctgattcaa cgtggtcaga tatacacgga cagagccgga taccctgtgg tgattactcg
     5641 cagtactcag cactcagtgt tctttcgacg catggacggg cgctccggac gggtacgcat
     5701 tggtgagttc aacaacctgt tcgaacatat tgaccaacag gagtaccgca aaattctggc
     5761 gggcactgag caggaaatgc gcctgaaaaa attacgcgca atgcaacgga ggtgatacat
     5821 gcatacggct tttgagttct gggttcgcaa gacattcggc aatcgctacg acctgacccg
     5881 tgatgtcgac ggcttctact gccgtgaagt tgtgaaacga atgtttgacg tgtggtgcca
     5941 ctgccgtgga tgaaagtttt atgaggttgg catgcagaca atcatctatc agataacccc
     6001 cagcaaatgg tgtacggaga gagtccttat tgcatcaaca gggctaaagc ccggcaccat
     6061 cgagcgggcc agaagaaagt catggatgca gggaaaagaa taccgccatt acgctgtaga
     6121 aggtgatcct gggcattaca gtgaatgcct gtacaacatc gaagaaatta tgcgatggat
     6181 cgaaaaccag aaacaaccag gtgccaaaaa tgcaagttcc ggttaacctg ttaatgctcc
     6241 tggacgtctg ggaggtttaa tgagtaacgc atcatacccg acaggcgttg aaaaccatgg
     6301 cggatcactc cgtatatggt ttcactataa tggcaaacgt gtcagagaaa acctcggtgt
     6361 tcctgacaca gccaaaaacc ggaagatcgc tggtgaactt cgcacttccg tttgttttgc
     6421 aatcagaatg gggagtttcg actacgccgc gcagttccct aattccccta acctgaaaca
     6481 ctttggtctg ggaaaaagag agataaccgt taaggcactt tcggaaaaat ggttggacct
     6541 taagaaaatt gagatttgtg cgaatgcact taaccgttac cagtcagtaa ttaaaaacat
     6601 gttaccaatg ttaggtgaaa aaaaactggt ttcatccata acaaaagagg atttactttt
     6661 cgtaaggaga gatttgttga ccggttacca aaagctttct aatggaaaga cttcttccat
     6721 aaaagggcgc tcagtggtca cggtaaacta ctatatgaca accatagctg gaatgtttca
     6781 atttgcaaca gataatggtt atacctcagg aaacccattt aacggtctgg ctcccttaaa
     6841 aaagtccaag gtaaaaccag atcctctcac ccgtgacgaa tttattcgtt ttattgaggc
     6901 ttgccgtcat caacaaacaa aaaacctgtg gattctcgct gtatacacgg gtattcgtca
     6961 cggggagttg gtatcgctgg catgggaaga tatagacctt aaagcaagga ctataaccat
     7021 ccgtagaaat tatacaaaac ttggcgaatt cactccacca aaaaccgatg caggcaccgg
     7081 aaggacaatt catctggttc aaccagctat tgatgctctt aaaagccagg cggaaatgac
     7141 catgcttgga aagcaacatt ctgtagaggt gaagcagagg gaatatggga gaactgctgt
     7201 gcataaatgc acttttgttt ttagtcctca ggtaacaaaa cagcagcagt tgtccggacc
     7261 tcactacaag gttgactcca tcagggagtc atggacaagt atcttaaaac gcgcaggtct
     7321 gagacacaga aaatcgtacc aatccaggca tacttatgca tgctggtcac ttgccgcagg
     7381 agctaatcct agttttatcg caagccagat gggccacaca aacgcacaaa tggtattcaa
     7441 tgtttacgga gcatggatga aagacaacaa tcacgaacag atagaactcc ttaacaaaag
     7501 actatctgaa agtgtcccat gtatgcccca taagaaagca gggtaaaata aaaacttgca
     7561 aaatcaatta gtttaccctt aatccctgtc acgttacgcg cgtggcagag gcgttacgga
     7621 ttgctgaaac cgcaacggac agacggcggt catcggctgt tcaacgatgc cgatattgac
     7681 cggatccgcg agatcaaacg ctggatcgac aacggcgtgc aggtcagcaa agttaaaatg
     7741 ctgctcagta atgaaaatgt tgatgtgcag aacggctggc gcgatcagca agaaacatta
     7801 ctgacctacc tgcaaagcgg caatctgcat agcctgcgaa cgtggatcaa agagcgcggt
     7861 caggattacc ccgcccagac gctcaccaca catctgttta ttcctctgcg ccgacggctt
     7921 cagtaccaac aaccgactct ccaggcgctg ctggcgatcc tcgacggcgt actgatcaac
     7981 tacatcgcca tttgtctggc ttcggcacgt aaaaaacagg gtaaagatgc gctggtggtt
     8041 ggctggaata ttcaggatac cacccgtctg tggctggagg gctggattgc cagtcaacaa
     8101 ggatggcgca ttgatgtcct cgcccactcg ctcaatcaac tacgccctga actattcgaa
     8161 ggccgtacat tgctggtgtg gtgcggtgaa aatcgaacct ccgcccaaca gcagcaactc
     8221 accagttggc aagaacaagg ccatgatatt ttcccactcg gcatttaatg attcgttaac
     8281 aaatgcgctt tactgtacaa tcctttcgtt aacataagga gtgcattatg cgcatagcta
     8341 aaattggggt catcgccctg ttcctgttta tggcgttagg cggaattggt ggcgtcatgc
     8401 tcgcaggtta tacctttatt ttgcgtgctg gctaagcgcc tgcaccagcc tttcaaacag
     8461 gcggtctgcg atgatcgccg ccagtgccac cagtaacgcc ccctggatca catacgcggt
     8521 attaaatccg ctaagcccga tgatgatggg cgtacccagc gtgctggccc ctaccgttga
     8581 ggcgatcgtc gccgtaccaa tgttgataat caccgaggtt cgcacgcccg ccagaatcac
     8641 cggagccgcc agcggtagct cgaccttacg cagtcgctga cctcgactca ttcccatacc
     8701 tttcgcaact tctgtcacgc tggcatcaat cgctcccagc ccggcaagtg tcgcctgcag
     8761 gacgggcagc acaccataaa ggatcaaggc gataatcgct ggttgcagac caaagcctat
     8821 caccggaaca gcaatcgcca gcactgcgac aggcggaaaa gtctgcccaa cggcggcaat
     8881 agtttccacc agtgggcgaa attccgcgcc ccacgggcga gtgactgcaa ttccggcacc
     8941 agtgccaatg atcaccgcaa acaaactcga aattcccacc agccagaaat gagccagtgc
     9001 caaagctgca aaactttctt gctgataaac gggtcgtggc agttgtggga acaaggcagc
     9061 aaacagcggc tggctgtaag gcagccagaa aatcagtgcc acaaacaaag caatgagcca
     9121 gaacagcgga tcgcgcaaca tcttcatacg cttacgcctc caccagcaga tcctgaaaat
     9181 gcagcgtgcc gctaggctgg ccctgcgtgt tcaccaccgg cagcacctcg catccccgcg
     9241 cgacaaacag ggagagcgca tcgcgcagcg tcatctcttc tgccagtgcc tcaccttctg
     9301 cccgttcttc gcgacgcacg taatccgcca cactacgtaa cgaaagcagg cgcacaccca
     9361 gttcactacg tccaaaaaac tggcggacaa aatcattcgc cggacgagtc agcatcgtca
     9421 gcggattccc ctgctgcacc acttcaccgt gatccatcaa taccagatgt tctgccagcc
     9481 gtagcgcctc atcaatatca tgcgtgacca gcacaatggt acgccccagc aaacggtgaa
     9541 tgcgcgtcat ctcttgttgc aacgcgccgc gcgttaccgg gtccagtgcg ccaaaaggtt
     9601 catccatcag taagacttgc ggatcggcag ccagtgcgcg cgctactccc acacgttgct
     9661 gctgaccacc ggaaagctga tgtggataac gctcacgtaa atttgactcc agccccagta
     9721 gcgccattaa ttcgtcgata cgatcgtcaa tcctcgcccg cgaccatttt tgtaattgcg
     9781 gcacggtggc aatgttttgt gccacgctcc agtggggaaa caggccaata gattgaatgg
     9841 catagcccat acggcggcgc aactccagta ctggcagcga gcgaatttct tctccagcaa
     9901 agcggagctc tccgctgtca tgttccacca ggcggttaat cattttcagg gtggtggatt
     9961 tgccggagcc agatgtgcca atcagcaccg aaaaactccc ttcctgaaaa ttgagattga
    10021 gatcgttaac ggctttttgt gcgccgaaca gtttgctgac atggctaaat tcaatcatta
    10081 cgtttcacct tcaacagtgc gattagtaaa tcgaacagcg catcgatcag caccgccaga
    10141 acaattaccg ggatcacccc cagcaacact aaatcaatgg cgctgcttag cagcccctgg
    10201 aaaaccagcg caccaaaacc gcctgcgccg attaacgccg caatcaccgc catgcctaca
    10261 gtttgcacca tcaccacccg caggctgcgc agaaataccg gtaacgccag cggtaactga
    10321 acatgcagga atcgctgcgc cccgctcatg cccatcgctc tggcgctctc cagcacatcg
    10381 cgcgggatct ggttcaagcc gactaccacg ccgcgcacca gcggtagcaa ggcatagagc
    10441 accagcgcaa tcagtgcggg tgtcattccg gttcctgcta tgccaagctt ccccagccac
    10501 ggaaaggccg taaccagtcc ggcaagcggc gcaatcagca ggccaaagag cgccacggaa
    10561 ggcacggtct gaatgacatt gagcagagaa aaaatcgccc cctgccgcgc ggtggaaaag
    10621 tagcaccaga tgcccaacgg cacaccaatc actaacgcag gcagcaccgc accaaacagc
    10681 aacgtcagat gttgtgccag cgcgtcgtca aacacatcct gacggttggc gtattctttc
    10741 attagtgaga gatcgttaag cgtgccggag tacagcaacc acagcggaat aatggcaatc
    10801 tgcatatgca acaaccagcg ccacagcgga tgcgtggaga ttcggcggat ggcatcgcta
    10861 caggccagca atgccagcgc cgaagccaac cagaaaccac tgccgaggct ggtacgcgcc
    10921 agcgcactgc cattttgcgc cagttgggtc gccgcctttc cagctcccca caccagcaat
    10981 acgaagacga attgcgccag aatgagtgca aaaatgctgc cttttttagc gggaataaaa
    11041 caggctgtca gccaggcgca accaacgccc accagcatcc agatcgtttg cggccacagt
    11101 tgccagagat gacgcccttc acccgagact aaacgattag gcgcgtagct gataaacggc
    11161 agcgctgctg cgattgccgt cagcaacagc agcagcgcca gaacaggatt aatacgtaga
    11221 taagtcacgg gaaattactt cgtccaccct ttttgtttca agtagtcggc agccactttt
    11281 ttggcatcca gtccttccac tgcaatgctg gcattcagtt gctgcaatgt ttttgcatcg
    11341 aggctggcga agactggctg tagccactgt gccatttgcg gatactcttt cagcaccgac
    11401 tcacgcacca ctggtgcagg cgcgtagata ggttgcacgc cttgcggatc gcttaaggtt
    11461 tgcagcccct gcgccgcgac cggaccgtca gtgccgtaag ccattgcggc attaacgcca
    11521 gaagtttgct gggcagcggc tttaatcgtt accgccgtgt cgccgccagc cagtgacagt
    11581 aactgatcct gatcgagttt aaagtcgtag gctttttcaa acgcgggtaa cgcatcggcg
    11641 cgttcgataa actcagccga ggctgccagt ttgaaggtgc cgccctcttt cagataacgc
    11701 ctcaggtcag caagcgaagt gagtttgttt ttctctgcca catcctgacg cacggcgatg
    11761 gtccaggtgt tatttgcagg tgcgggcgtc agccagatta acttgttttg ctctgcatcg
    11821 agttttttga ctttctcgta accttgctgc gcgtttttcc acgctgcatc gttttcatct
    11881 ttaaagaaaa acgcgccatt gccggtatat tccggataga tatccagttc accggaagta
    11941 atcgccccgc gcaccacagg agtcgttcca agttgcactt tattgaccgt tggtactccg
    12001 tggctttcga gtacttgcaa aatgatattg ccgagcagcg cgccttcggt atcgattttt
    12061 gaaccgactt taacggggga agccgcgtgc agcggcaggc tcacggctgc caacaaaacc
    12121 agtgaacctg cccagacctt tgagagtggc atgatgcttt cctcattctt ttactgttgt
    12181 tttcagcgaa ttaagagaaa agcatagttg ataatggcgg gattagcctg agcggacgga
    12241 ttcagttgca gaatcagata aatacttaag aggcatattc ggtccggcgt ttcctgccgg
    12301 atgcggcgcg agcgccttaa ccggcctaca aagggcgaaa attcagcaag ttaagatatg
    12361 aacatcaggc aaagggcgca aacgtcgcgc ccttgctaaa gcattacagc aactcaaact
    12421 cgcctttctt aacgcgtgcg gaatcagtgc cgataaagac attgaacttg ccaggctcgg
    12481 cgtcatattt catctgttga ttccagaact tcagcgcctc gatatcaatc gggaagctga
    12541 cggtctgggt ttcgcccggc ttcagggtga ttttctcaaa gcctttcagc tgcttcactg
    12601 gacggctcat ggaagccgtc acatcctgca agtacatctg cactaccgtg gccccttcgc
    12661 gtttaccggt gttcgtcacc tgcacgctgg ccgtcacttt gccgtcacgc ttcatggtcg
    12721 gcgcagaaag tttcacatca gagacggtga aagtggtata gctcagacca tagccgaacg
    12781 gataaagcgc cccgttagct tcatcaaaat aacgcgaagt gtatttgttc ggcttgtcgg
    12841 cattatacgg acgaccggtg ttcagatggc tgtagtacac cgggatctgc ccgacagaac
    12901 gcgggaagga catcggcagc ttaccggacg ggttgtaatc gccaaacaac acatcggcaa
    12961 ttgcattacc gccttcagtc ccggcaaacc aggtttccag aatcgcatca gcctgctgat
    13021 cttctttcac cagcgccagc ggacgcccgt tcatcagcac cagcaccagc ggtttgccgg
    13081 tggctttcag ggcggcaatc aaatcacgct ggctttgcag aatagtaata tcggtacggc
    13141 tggaggcctc gtgcgccatc ccctgtgctt caccgactac agccaccacc acatcagatt
    13201 gtttcgccgt ctgcaccgct tcatcaatca tctcttgcgg cgaacgcgga tcgactttga
    13261 ccgcttcttc atactgattc aggaaatcga taatgccttt gtcactggta acgttcgccc
    13321 ctttggcata cagcacttta ccgttttcac cgacggcatt tttaatcccg gtcagcacgg
    13381 tcacggattg atcggcaaca cctgccgccg accagctgcc catcacgtca cgtttactgt
    13441 ccgccagtgg cccaaccacc gcaatggtgg ctgatttttt cagcggtaac gtttcgagac
    13501 ggtttttcag caacaccagg ctttcacgcg ccacttcacg cgcttctttg cggtgcaggc
    13561 ggctttcggc attggtatcc accgggtcag actctttcgg accgagatgg ctgtatgggt
    13621 cgttaaacaa ccccatatca tatttaacgt tcagtacatg gcgggcagca tcgtccagct
    13681 ctgccatcgt cactttaccg gatttgatca acccaggcag atacttcgag tagtactcgt
    13741 cgctcatgct catgttgatt ccggatttca gcgccacgcg cactgcatct tccggatctg
    13801 ccgccgtgcc atgtttaatc agctctttga ttgcaccgtg atcggaaacg gtgatgcctt
    13861 taaagcccca ctggtcgcgc agaacatctt tcagcagcca ggagtcggag gtggctggcg
    13921 tgccgttcag cgagttcagc gccaccatca ccgcgccgct gcctgcgtcc agccccgctt
    13981 tgtacggcgg catataatca ttaaacaggc gctgcggact catatcgacg gtgttgtact
    14041 ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt gacgctggtc atcaccgagt
    14101 aacgatctgc cgggctttta ccctgcatcg cttccaccat ggttttaccc attattgagg
    14161 tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc ccagcgcgga tcgcgcgaga
    14221 catcgaccat cggtgcccag gtcatattca ggccatcatc tgccgcttca taagcagaga
    14281 cacgtccgac cgtttttact gcatcgaggt taaaagacga ggccagaccg aggctaatcg
    14341 ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa gaaaagagga attttcaggc
    14401 ggcttaattc catcacctga tcctgcatgg cgcggatatc ctgacgggtt acggtgttga
    14461 aaatagcccc aacctgaccg tctttgatca tctcgcggat cgcctctttc gggttatccg
    14521 ggccgacgct gattaagcgc agctgaccaa ttttctcatc aactgtcatt ttcttaagca
    14581 gttcggtgac gaacgcatcc cgcgcttcag gcgttaatgg atggttaccg aataaatcat
    14641 ccgccagtgc tggctgcagg gccagactca ccgcgattcc tactgaacat agccatttca
    14701 tatggatttt tcttcctttg ttgcccgacg tggcagcgaa aatggtgcaa aaaccgtagt
    14761 ttgccataag catgatggag agagaaaaag aatgctcagt ttattgtctg aattttcaaa
    14821 atattcactc gctgaattgt catacaaggc gctattctag tttgtgatgt tttttcgcca
    14881 ccacaaggag tggaaaatgt cttccatgac aacaactgat aataaagcct ttttgaatga
    14941 acttgcccgt ctggtcggtc attcacacct gctcaccgat cccgcaaaaa cggcccgcta
    15001 tcgcaagggc ttccgttctg gtcatggcga cgcgctggct gtcgttttcc ctggctcact
    15061 actggaattg tggcgggtgc tgaaagcctg cgtcaccgcc gacaaaatta ttctgatgca
    15121 ggctgccaat acaggcctga ccgaaggatc gacgccaaac ggtaacgact atgatcgcga
    15181 tatcgtgatc atcagcaccc tacgtctcga caagctgcac gttctcggca agggcgaaca
    15241 ggtgctggcc tatccgggca ccacgcttta ttccctggaa aaagccctca aaccgctggg
    15301 acgcgaaccg cactcggtga ttggttcatc gtgcattggc gcatcggtta tcggcggtat
    15361 ctgtaataac tctggtggtt cgctggtgca gcgtggcccg gcgtataccg aaatgtcgtt
    15421 attcgcgcgt ataaatgaag atggcaaact gacgctggtg aaccatctgg ggattgatct
    15481 gggcgaaacg ccggagcaga tccttagcaa gctggatgac gatcgcatca aagatgacga
    15541 tgtgcgtcac gatggtcgtc acgcccacga ttatgactat gtccaccgcg ttcgtgatat
    15601 tgaagccgac acgcccgcac gttataacgc cgatccggat cggttatttg aatcttctgg
    15661 ttgcgccggg aagctggccg tctttgcagt acgtcttgat accttcgaag cggaaaaaaa
    15721 tcagcaggtg ttttatatcg gcaccaacca gccggaagtg ctgaccgaaa tccgccgtca
    15781 tattctggct aacttcgaaa atctgccggt tgccggggaa tatatgcacc gggatatcta
    15841 cgatattgcg gaaaaatacg gcaaagacac cttcctgatg attgataagt taggcaccga
    15901 caagatgccg ttcttcttta atctcaaggg acgcaccgat gcgatgctgg agaaagtgaa
    15961 attcttccgt ccgcatttta ctgaccgtgc gatgcaaaaa ttcggtcacc tgttccccag
    16021 ccatttaccg ccgcgcatga aaaactggcg cgataaatac gagcatcatc tgctgttaaa
    16081 aatggcgggc gatggcgtgg gcgaagccaa atcgtggctg gtggattatt tcaaacaggc
    16141 cgaaggcgat ttctttgtct gtacgccgga ggaaggcagc aaagcgtttt tacaccgttt
    16201 cgccgctgcg ggcgcagcaa ttcgttatca ggcggtgcat tccgatgaag tcgaagacat
    16261 tctggcgctg gatatcgctc tgcggcgtaa cgacaccgag tggtatgagc atttaccgcc
    16321 ggagatcgac agccagctgg tgcacaagct ctattacggc cattttatgt gctatgtctt
    16381 ccatcaggat tacatcgtta aaaaaggcgt ggatgtgcat gcgttgaaag aacagatgct
    16441 ggagctgcta cagcagcgcg gcgcgcaata ccctgccgaa cataacgtcg gtcatttgta
    16501 taaagcaccg gagacattgc agaagtttta tcgcgagaac gatccgacca acagtatgaa
    16561 tccggggatc ggtaaaacca gtaagcggaa aaactggcag gaagtggagt aaaaattacg
    16621 gatggcagag tatcgccatc cgaattcact taatcgttct gtgccgtctg ccccgccgcc
    16681 gccatttggg cggctttttg ttttttatag cttaacgctg ctgccggaac aggcatcact
    16741 ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt gtatttgcca
    16801 aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg catcaccaga
    16861 caatggcccg ccgcattggt aaagccggtt ttggttagct ggatattcca gttatcgcga
    16921 tacaccagat gattagtatt gcggaacggc agcgtatacg tcgggttaga gaaggttgcc
    16981 atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct ggcaatgagc
    17041 agtttggtta agtcacgggc ggttgaaacg ttatgcaccg acaatccggt aggttcaaca
    17101 aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt aataaaggct
    17161 ttgtaaccac cgggataatg gtgcgcaagg cttgccgccg cgcggttttc tgaagacatc
    17221 agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac gcgcgaatag
    17281 acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc cagcggcagt
    17341 cgtgcatcca gcacgaccat cgcagtcatt aatttgctga tagacgcaat cggacgcacc
    17401 agatccgggt ggttcgaata gatcactttg ttggtattca gatcaacaat catcgcgcta
    17461 ccggaggcaa tttccggttg tgaagcggtg gtagcggctg ccgttttcgc aacggcctgc
    17521 ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg aaatttcggc
    17581 atgatgagca ttcagatagt ggttcacgcg cacgggttgc gcaccgccgg agtaaggatt
    17641 tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa ggagaatcgt
    17701 gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga actttctaga
    17761 acaaacgata accgtagccc catagtataa cggtaagggc gagcagcact tccagtacca
    17821 gcacgccaat cgccagcgtc gaactggaga agctaaggcc ttcctctttg ttgatattca
    17881 ggaagctcgg aataccaagg taaagtaggt aaccggtgta aaacagtgcc accgtgccga
    17941 ccagcgcaca caaccagacc agcggataaa gcgccaccag accacttaaa aacagcggag
    18001 ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc ggataattac
    18061 gcgccatcca ccagatgacc cgccccatca ccgcgacccc agcgagcata acgccataaa
    18121 acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg ccatcgccaa
    18181 aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc gccgccatca
    18241 gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta atcacctgca
    18301 tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc ccttgttgtg
    18361 agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt gttcgcccat
    18421 gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgattcaca gggtgtatgc
    18481 ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc atctcacaat
    18541 atggttatgc cgcgctggtt atcggtagcc tggcggaagg tgaaaccgtg actttgctgg
    18601 gaggccttgc ggcacatcag gggctattaa agttcccgct ggtggtactt tctgtagcgc
    18661 ttggcggcat gattggcgac caggtgctct atctgtgcgg gcggcggttt ggcggtaagc
    18721 tgttacgccg tttctctaaa catcaggata aaattgagcg ggcgcagaaa ctgatccaac
    18781 gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg gtgattggcc
    18841 cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctt aatattctcg
    18901 gcgcatttgc ctgggcgttg atttttacca ctatcggtta cgctggtggt caggtgattg
    18961 cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg attctggtcg
    19021 tggttctggt ggtaggcgtg cgctggtggc tgaaacgacg cgggaagaaa aagccggata
    19081 atcaggcgta aaaccattgc cctggataag gcgtgcgcgc cgcatccgac atccggttca
    19141 ccatgcctga tgcggcgctt gtgcgtctta tcatgcctac caaaatttta cccattgaaa
    19201 tatccacaca cctcactgcg ctattctggg ttgaactgcg gattcgccaa cataaagccg
    19261 ccatccacta tcaacgactg cccggtggtg taatttgcgc cctccgaaca aagccacgcc
    19321 accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga aggctccgcg
    19381 tcgggcttca cgtcgctatc atccatgccg ttcattggcg tagcgatcgc cccaggtgcg
    19441 actgcgttca ccaaaatctt atgcctgacc aactccagcg ccattgcttt ggttaacccg
    19501 ccgagcgcat gtttagcggc tgtgtaggcg ctggcatccg gcagcggcgt atgttcatgt
    19561 accgacgtaa tgttgatgat gcgaccaccc tgtccttgtt tcaccatctg gcgggcagca
    19621 atttgcgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg ccactcatcg
    19681 aaagccatat caagaaacgg cactttggtc attgcgcccg cattattcac cagcacatca
    19741 atacgcccca gccgttgaat aagtttctcc agcgcctgcg caccttctgg cagattgccg
    19801 agatccagtt gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg cgcggtatct
    19861 tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccttg ctgcgccagt
    19921 aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc tacctgtgcc
    19981 atcgagttct ccacttaacg ctgaataaac gttaagtata gaagtcacat atcatcagcg
    20041 tttgtaaccc ccgcccaacg caccagtgag ttgaatggag gcatccagcc actgcccttg
    20101 caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc tgacgcggga
    20161 accagcaatg atgcccgcgt taaagcgggc ctgcgcaaga cctaccacac gcaaggcatc
    20221 gcgctcaatt tgcgcctgat gctggttttt ctccgccagt gtctgaacct gactggctgc
    20281 ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga tagacaagtt
    20341 gctttcggct tttgcgatat cgagattggc gttaagacga ccactatcga aaatgggtag
    20401 cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat cgcttaagtg
    20461 caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg ccgcttttgc
    20521 cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca aatctgcccg
    20581 ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt tcggcaacgc
    20641 caccggttta agcgtcaatg acttagtctg gtgatttgtg agtgcaatta accgtgcctc
    20701 aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc tggcattaat
    20761 atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct ggcgatcggt
    20821 cgcgataatg gtgttctgct ctttttcgat ttgctgcaag accgtgttta acgccgcctg
    20881 ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc gggtttgctc
    20941 gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg cccgattctt
    21001 tccccagata tcgagatgcc agcccgccgt taagccaaaa gttccgttgg tgtaccacgg
    21061 accggtcgta cctgcggccg gatcgttcag agcaaacggc cccattagcc cttctgccga
    21121 cattttttgc cgctccatat ccgcagaaaa gtcgatctgc ggaccatcct gagtagcaac
    21181 tgccttcgcc tgggcttcag ctaactgaat gcgctgttca gccacctgca tatccggtgc
    21241 gttctgtagt gcattgttaa ttaaggaagt gagttgatta tcgtgatact ccagccacca
    21301 ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa cttgtgcagc
    21361 gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc cggccagcat
    21421 cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca tgggggaata
    21481 atcaggtaag aaaaggtgcg cggagattac cgtgtgttgc gatatatttt ttagtttcgc
    21541 gtggcaatac atcagtagca ataaaacgac atatccagaa aaatatacac taagtgaatg
    21601 atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg ttttttccta
    21661 tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct gggctgtatt
    21721 gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga tattttaaaa
    21781 atcagatatg acatataagt tgtcaggtca ggaactagat attttgtgta cttgatttgc
    21841 aaacagctcc ggtgttggtg gcgtcaaaat cgctgaagcg ttccccgaag gccggggcag
    21901 cccacatgga tgtgggctga gggcgcgtct tacaagga
//
LOCUS       DAZFDC010000080        21901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFDC010000080 DAZFDC010000000
VERSION     DAZFDC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.080"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            103..378
                     /gene="ytcA"
                     /locus_tag="ACHJ48_003070"
     CDS             103..378
                     /gene="ytcA"
                     /locus_tag="ACHJ48_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO4708575.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANIKLAFVGIIYTALFALYAMLFWLAFF"
     gene            397..1428
                     /gene="mdtN"
                     /locus_tag="ACHJ48_003071"
     CDS             397..1428
                     /gene="mdtN"
                     /locus_tag="ACHJ48_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO4708576.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKSEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1428..3492
                     /gene="mdtO"
                     /locus_tag="ACHJ48_003072"
                     /pseudo
     CDS             1428..3492
                     /gene="mdtO"
                     /locus_tag="ACHJ48_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            3489..4955
                     /gene="mdtP"
                     /locus_tag="ACHJ48_003073"
     CDS             3489..4955
                     /gene="mdtP"
                     /locus_tag="ACHJ48_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO4708577.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVTAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGSGYQAAPVVEKK"
     gene            5153..7300
                     /gene="fdhF"
                     /locus_tag="ACHJ48_003074"
     CDS             5153..7300
                     /gene="fdhF"
                     /locus_tag="ACHJ48_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5570..5572,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase N subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFO4708578.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLSIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7394..8083
                     /gene="yjcO"
                     /locus_tag="ACHJ48_003075"
     CDS             7394..8083
                     /gene="yjcO"
                     /locus_tag="ACHJ48_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO4708579.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(8261..9574)
                     /gene="gltP"
                     /locus_tag="ACHJ48_003076"
     CDS             complement(8261..9574)
                     /gene="gltP"
                     /locus_tag="ACHJ48_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO4708580.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9916..10512)
                     /gene="nrfG"
                     /locus_tag="ACHJ48_003077"
     CDS             complement(9916..10512)
                     /gene="nrfG"
                     /locus_tag="ACHJ48_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO4708581.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQNPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10509..10892)
                     /gene="nrfF"
                     /locus_tag="ACHJ48_003078"
     CDS             complement(10509..10892)
                     /gene="nrfF"
                     /locus_tag="ACHJ48_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO4708582.1"
                     /translation="MYKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10885..12603)
                     /gene="nrfE"
                     /locus_tag="ACHJ48_003079"
     CDS             complement(10885..12603)
                     /gene="nrfE"
                     /locus_tag="ACHJ48_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO4708583.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWKLKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGILLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDV"
     gene            complement(12623..13579)
                     /gene="nrfD"
                     /locus_tag="ACHJ48_003080"
     CDS             complement(12623..13579)
                     /gene="nrfD"
                     /locus_tag="ACHJ48_003080"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO4708584.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLSLVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLAAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LFKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13576..14247)
                     /gene="nrfC"
                     /locus_tag="ACHJ48_003081"
     CDS             complement(13576..14247)
                     /gene="nrfC"
                     /locus_tag="ACHJ48_003081"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO4708585.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(14244..14810)
                     /gene="nrfB"
                     /locus_tag="ACHJ48_003082"
     CDS             complement(14244..14810)
                     /gene="nrfB"
                     /locus_tag="ACHJ48_003082"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO4708586.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14855..16291)
                     /gene="nrfA"
                     /locus_tag="ACHJ48_003083"
     CDS             complement(14855..16291)
                     /gene="nrfA"
                     /locus_tag="ACHJ48_003083"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO4708587.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16684..18642
                     /gene="acs"
                     /locus_tag="ACHJ48_003084"
     CDS             16684..18642
                     /gene="acs"
                     /locus_tag="ACHJ48_003084"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO4708588.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDQVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDS
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18843..19157
                     /gene="yjcH"
                     /locus_tag="ACHJ48_003085"
     CDS             18843..19157
                     /gene="yjcH"
                     /locus_tag="ACHJ48_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO4708589.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            19154..20803
                     /gene="actP"
                     /locus_tag="ACHJ48_003086"
     CDS             19154..20803
                     /gene="actP"
                     /locus_tag="ACHJ48_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO4708590.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20983..>21901
                     /gene="espX5"
                     /locus_tag="ACHJ48_003087"
     CDS             20983..>21901
                     /gene="espX5"
                     /locus_tag="ACHJ48_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFO4708591.1"
                     /translation="MRYNGLNNMFFPLCLINDNHSVTSLSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIDLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIASYANNK
                     CDDRITRRVYLNKDKEVSIKVVYFINNVTVHNNTIEIPQTVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFNFAIMEKILFDNCILDDSYFAQIKMTDGT
                     LNSCSAMHVQFYNATMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYINLFRADLSF
                     SKLDLINFKHADLSRVNLNKAILQNINLID"
BASE COUNT         5330 a         5877 c         5664 g         5030 t
ORIGIN      
        1 taaagctatt tttattcatc aagaaaatag ccatactgac gcattcaatg gatattgata
       61 ttcactacag atattgttcg cagcagacag gatgtattta atatgccaac agttctctct
      121 cgcatggcga tgcaactcaa aaaaacagcc tggataattc ccgtcttcat ggtttcggga
      181 tgctcattat ctccggcaat cccggtgatc ggcgcttatt atcccagctg gtttttctgc
      241 gccattgcca gccttatatt gacgctcatc acgaggcgag ttattcagcg ggcaaatatc
      301 aagctggcat ttgtcggaat tatttatacc gccctttttg ctctctacgc catgctgttc
      361 tggctggcat ttttctaatc tatttgagac gcgctcatgg aaagtacgcc gaaaaaagct
      421 cctcgcagta aattccctgc tctgttagtg gttgcgttgg cgctggttgc ccttgttttc
      481 gttatctggc gcgtagacag tgcgccatca actaatgacg cttacgtgtc agcagatacc
      541 attgatgtgg tgccggaagt cagcggccgc attgtagaac tggcggtcac cgacaaccag
      601 gcagtcaaac agggcgattt gctgttccgc attgacccgc gcccgtacga agccaatctg
      661 gcgaaatctg aagcctccct cgcggcactg gataagcaaa ttatgctcac ccagcgtagc
      721 gttgacgcgc aacagtttgg tgccgactcg gttaacgcca cggtagaaaa agcccgtgcc
      781 gccgctaaac aggccacaga tacattacgc cgcaccgagc cattactgaa agaaggtttt
      841 gtctcagcgg aagacgttga ccgtgcgagg accgcgcagc gcgctgcaga agccgatctt
      901 aatgctgtat tgttgcaggc gcagtcagcc gccagcgccg tcagcggcgt ggatgcatta
      961 gttgcccagc gtgcggcggt cgaagcggat attgccctga ccaaactgca tctggaaatg
     1021 gcgaccgttc gcgcaccgtt tgatggccgg gtcatttccc tcaaaacctc cgtcgggcaa
     1081 tttgcttctg ccatgcgccc tatttttacc ctaatcgaca ctcgtcactg gtacgtgatc
     1141 gccaacttcc gcgaaaccga tctgaaaaat attcgctcag gtacacctgc aacgattcgc
     1201 ctgatgagtg acagcggcaa aactttcgag ggtaaagtgg attcaattgg ctacggcgtg
     1261 ctaccggatg acggcggcct ggtgctgggc ggcctgccga aagtttctcg ttctattaac
     1321 tgggtccgtg ttgcccagcg ttttccggtc aaaatcatgg ttgataaacc tgacccggaa
     1381 atgttccgca tcggcgcttc ggcagtcgct aatcttgagc cgcaataatg agcgcgctca
     1441 actccctgcc attaccggtg gtcaggctgc tggcgttctt tcatgaagag ttaagcgagc
     1501 ggcgaccagg tcgcgtgccg cagaccgtgc aactctgggt aggctgcctg ctggtgattc
     1561 tgatctcgat gacttttgag atcccttttg tggcgttatc gctggcagtg ctgttttacg
     1621 gtattcagtc gaacgcgttt tacaccaaat ttgtcgcgat cttgtttgtg gttgccacgg
     1681 tgctggagat cggcagcctg tttttgatct acaaatggtc atacggcgaa ccgttgatcc
     1741 gcttgatcat cgccgggccg atcctgatgg gctgcatgtt tttgatgcgc acccatcggt
     1801 tgggactggt ctttttcgct gtcgccattg tcgccattta tgggcaaacc ttccccgcca
     1861 tgctcgacta tccggaagtg gtcgtgcgct taacgctgtg gtgtatcgtt gttggcctct
     1921 acccgacgtt attaatgacg ttaatcggcg tactgtggtt tcccagtcgt gccatttcgc
     1981 aaatgcatca agcgcttaat gatcggcttg atgatgccat tagccacctg acggacagcc
     2041 tcgcaccgct acccgaaacg cggattgaaa gagaggcgct ggcgctgcaa aaactcaatg
     2101 tcttttgcct cgcggacgat gccaactggc gaactcaaag cgcatggtgg caaagctgcg
     2161 tggcaacggt aacctacatt tactcgacgc tgaatcgcta cgatcccacc tcttttgctg
     2221 attctcaggc aattattgaa ttccgacaaa aattagcttc agaaatcaac aagctgcagc
     2281 atgccattac cgaaggtcag tgctggcaaa gcgactggcg gatcagtgaa agtgaagcga
     2341 tggcggcacg ggaatgtaac ctggagaata tctgccagac gttgttacaa ctgggtcaga
     2401 tggacccgaa tacgccgcca acgcccgccg ccaacgcccg ccgccaaacc gccatcaatg
     2461 gtcgctgatg cttttaccaa tccagactat atgcgctacg cggtaaaaac gctgctcgcc
     2521 tgtttgatct gttacacctt ctacagcggc gtggactggg aaggcattca cacctgtatg
     2581 ctgacctgcg tgatcgtcgc taatccgaat gtcggttcat cgtaccagaa gatggtgctg
     2641 cgttttggcg gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg
     2701 ccctggctgg acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc
     2761 gcatggattg ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc
     2821 ttcgcgctcg ccacgctcga aaacgttttt ggtccggtgt acgacctggt ggaaattcgc
     2881 gatcgcgccc tgggtatcat cattggtacc gtggtgtctg cggtgattta cacctttgtc
     2941 tggcctgaaa gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgcta
     3001 agtaaagtaa tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa
     3061 attcgtatag gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg
     3121 ctggagcgtc aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt
     3181 attcatcagg gccgcgatct tcttcacgcc tgggatgcca cctggaactc ggcgcaggcg
     3241 ctggataacg cactacagcc ggacaaagca ggtcagtttg ccgacgccct ggaaaaatac
     3301 gctgccggtc tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc
     3361 gcctcgcagg ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc
     3421 gcccgcttgc cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga
     3481 gccacgcaat gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca
     3541 cgacgctgat ttccggctgt gccctggtac gcaaggattc tgcgcctcat caacagctca
     3601 aaccggaaca aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg
     3661 cgcagtggtg gaaacaactc aatgacccgc agctggatgc gctgatccaa cggacgctaa
     3721 gtggttcaca caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcag tcccaggccg
     3781 atttgttaga tgccggttca caattacagg tcgcagcgtt agggatgctc aaccgccaac
     3841 gcgtctcggc gaacggcttt ttaagccctt atgcgatgga tgcgccagca ctgggtatgg
     3901 acgggccgta ctatacggaa gccacagtcg gtttgtttgc cggactggat cttgatttgt
     3961 ggggtgtgca tcgctcagcg gttaccgccg ccattggcgc gcataatgcc gcgctggcag
     4021 aaaccgcagc agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc
     4081 aggccagcta tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg
     4141 tgaaagcgca ccaaagtaaa gtggcgcacg gtctggaagc gcaagtgcct ttccacggcg
     4201 cgcgggcaca gattctggcg gtcgataaac aaattgctgc cgttaaaggg caaatcaccg
     4261 aaacgcgaga atctctgcgt gcattgattg gcgcgggagc cagcgatatg ccggagatca
     4321 aaccagtggc attaccacga gtccagaccg gtattccggc gacactctct tatgagttgc
     4381 tcgccagacg cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg
     4441 tggattccgc gcgggcgctg ttctacccaa gctttgatat caaagcgttt ttcggtctgg
     4501 actccatcca cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg
     4561 gtctgaaatt gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcactcgcg
     4621 ccgccagcaa catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg
     4681 ttgccgtcaa cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac
     4741 gcgtggaagc aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa
     4801 ccagccgctt acaggctacc gaagcccggt tgccggtgct tgccgaagag atgtcattac
     4861 tgatgctgga cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcagcg
     4921 ggtatcaggc ggctcccgtc gtcgagaaaa aataaaatgt ctgccgtgtg atggctgtca
     4981 cgcggtattt cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg
     5041 aataaatagt gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc
     5101 atactgtagt cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa
     5161 agtcgtcacg gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa
     5221 cggcaaaatc gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct
     5281 gaagggttat tatggctggg acttcattaa cgatacccag atcctgaccc cgcgcctgaa
     5341 aacccccatg atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gggatgaggc
     5401 actgaattac gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat
     5461 ccagacgacc ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt
     5521 tgcgcgcgcc gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc
     5581 atcggttgca ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga
     5641 aattgataat accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat
     5701 cgtggcgaat cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc
     5761 gcgcaaaatt gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa
     5821 catcgcgctg ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc
     5881 attcgtcgct tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac
     5941 gccggagtcg gttgaagata tcaccggcgt cagcgccagt gagattcgtc aggcggcacg
     6001 gatgtatgcc caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta
     6061 ccagggcgtg gaaaccgtgc gttctctgac cagcctcgcg atgctgaccg gtaacctcgg
     6121 taagccacat gcgggtgtta acccggttcg tggtcagaac aacgtgcagg gtgcctgcga
     6181 tatgggcgcg ctgccggata cgtatccggg ataccagtac gtaaaagatc cggctaaccg
     6241 cgagaaattc gccaaagcct ggggcgtgga aagcctgcct gctcataccg gttatcgcat
     6301 cagcgagctg ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga
     6361 agatccgcta caaactgacg cggagctgtc ggcagtacgt aaagcctttg aagatctgga
     6421 actggttatc gttcaggaca tctttatgac caaaaccgcg tcggcggcgg atgttatttt
     6481 accgtcaacg tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca
     6541 gcgtttcttc aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag
     6601 tgaaatcgcc acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga
     6661 tgagttgcgt catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact
     6721 gggcttcatt cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct
     6781 gtttaaagag aagtttgaca ccccgaacgg tctggcgcag ttcttcacct gcgactgggt
     6841 agcgccaatc gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt
     6901 tggtcactac tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga
     6961 tgaacctggc tacgcacaaa tcaataccga agacgccaaa cgtctgagta ttgaagatga
     7021 ggcattggtt tgggtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga
     7081 tcgtccgaac aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga
     7141 gctggttacc gaaaacttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt
     7201 gcgcgtcgag ccgatcgccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa
     7261 caagttgaaa actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct
     7321 cctttcggag gctgtttttt tatccattcg aactctttat actggttact ccctacccaa
     7381 tcgtattatc aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca
     7441 cgccaacgac tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg
     7501 cgcacaatat tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata
     7561 ttgggcacag aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat
     7621 caaaatcacc aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc
     7681 ggcgcaagcg ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca
     7741 agcgggtaaa ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct
     7801 ggagaacgac tctgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt
     7861 gggcattaag gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc
     7921 ccgaaccggt tattccgagt actgggcggg aatgatgttc ttaaacggtg aagaaggttt
     7981 tatcgagaag aacaagcaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt
     8041 tgataccggg tgtgaagagt ttgaaaaatt aactaacggt taaggtaggc ctgataagac
     8101 gcgttaagcg tcgcatcagg cgttgatgtc ggatgcagcg taaacgcctt atcagaccta
     8161 caaaatcacg ctagcgctgt ctgacgtagg cctgataaga cgcaccagcg tcgcatcagg
     8221 catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc gcagttttat
     8281 caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca aatttgtgtt
     8341 cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagt gcagtacgcg
     8401 ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc ggaataccta
     8461 cgctacccag cgttgccagc aacaccacaa acgacacgcc cggcacgcca gcaatccctt
     8521 tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac aggtcaatgc
     8581 catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa ccatcaaggt
     8641 taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct ccgtaggctt
     8701 ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag tacgccagaa
     8761 tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcat aggcgcgcca
     8821 caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc agcaccagtt
     8881 tcgccagtgg ccacagagaa gagaaaccaa agttagccac cgtcactgca atcagcgcaa
     8941 acacacccac cggcgcataa cgcatcacca tgtgagtcac tttaaacatg gtttcagaga
     9001 tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggag gaaagcccca
     9061 gaccaaacag caccgagaag aagatgatcg gcaacatttc gcctttcgcc atcgacgcca
     9121 caatattcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa ctgctttgta
     9181 ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc gacatatcca
     9241 ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg gcaacggtgg
     9301 tgatcacctc gaagtagata atggttttcg cgccaatacg cccgagctgt ttggcatcac
     9361 caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca atcattttga
     9421 tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc cagtcgcggc
     9481 tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata gcaaacagaa
     9541 tctgccaggc caggctgaat tttatatttt tcatatgaat gacttcctca atggagcgcc
     9601 gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt gtgttgcgtt
     9661 ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg ttgcgcaacc
     9721 ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta tggcaggatt
     9781 aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat aatactgatt
     9841 agtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa taacataatc
     9901 ttcatcacca gatgattaat ccgatctccg ctgcaacaat ttcgccatat taatcgactc
     9961 gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt gccatagttc
    10021 gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca tcagagcggt
    10081 gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct gggcggtcat
    10141 atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt cagcgttctc
    10201 accacgtagt tgcaacgcct gacggtacgc cagcagcgaa ttgctgtaat cgttttgcca
    10261 cagatagtac tcgcccagta acgcccactg ttcgctgttt tgtggattag cgcggatttt
    10321 atcctgcaat gcctgaagct gcgcttccgg gttttgctgg ctggcaaact gatgtagcgg
    10381 atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta acagataact
    10441 accgatacag aggaatacca tcaatatcgt gagtgttgtc agcattttca ccggtatttg
    10501 aggctgtttc atcgcttcgc cctcactcgc cagaggatca gtgccatcag cagtaacaac
    10561 accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt atagcgaaca
    10621 aaatctccgt agcgttcggt catccagcca atgatttcga cttcactttt cccctccgcc
    10681 accatgctgt aaacctgatg gcgcatactg actgccaccg gcgcgttgga ttccagtaag
    10741 ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc ctgttgctgt
    10801 tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa acaggtaaat
    10861 aacagcagta gcgtgagaag ccctttatac atcgcgcttc ttcccccgcc atccgcttaa
    10921 caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac cgctttgtac
    10981 atacaagcga aaagcgtaac gatccgctcc agttttctcc cccatgaccg cataccagtc
    11041 atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacgacgag cttcgtaaaa
    11101 acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg ccactatcgc
    11161 tttttcgctg gtgtaattgc ctttggcttg cagatcgagg cgctcaaaac ggaaggtgta
    11221 tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct gacggctgac
    11281 gctggagaca acgatccctg cggcaaataa cagtatgccc gcatgcgcca gcagcgccgg
    11341 aagctgcgcg cgtttgccag agacaaacgt cgccagcaca atcaccacca acatcaacag
    11401 accaaacggt aacgtcgcgc ggttaaaata cggtgcgcca acggagagac gtccccagcc
    11461 cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta gcactgcgca
    11521 aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa aacgcaccgc
    11581 cgggccaccg tcccgcgctc gccagccata cagagccaga gacgcaaggc taatcagtgc
    11641 aaacaggctg aacaacggca cagcgcgaac gttatccaga gcgaacgcat gaaccgaaac
    11701 cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag taactatcgc
    11761 caacaacagc gaccagtggc gaaaaatccc ccgctggcgt gtcagggata aactgtgcag
    11821 cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat cccagaacca
    11881 ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga tccccgcagt
    11941 taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgcagg caccatcaaa
    12001 ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac cgccatagcc
    12061 aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg gattgagatc
    12121 gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca gcactacaaa
    12181 cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa atagcggatc
    12241 ggtttgctgc cgatattgcc aggcaaacag cgcgctccag gcggaaagca gcagcaccca
    12301 aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt tccacgacag
    12361 caagctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga gaaaacaata
    12421 cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgt gccagaatgc cgatgcaagt
    12481 gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca acggggtcaa
    12541 cacgttgacc ccgagactta acaacagcgc cagaaaaccg acttcaggaa ggaatacgtc
    12601 cagaaaccac ctctgttctg gcttacgcca ccgttaactg cccggcgtag agaatgaaaa
    12661 atcgcaacat caacacgccg accagactgg ccccacacgc cgccagcacg gcaggaatgc
    12721 cggaactgcg attgacccac ggtttgaaca acattggcac aatcagcccc agcccggcga
    12781 caccaagcca gaaccaccac gtccagaaac cgccgcccaa tgccgccacc agcgcacgca
    12841 ctttaccgtc atcgcccagc gccagaccga caaaaaacgc cgccagcagg aagatttcac
    12901 cccataccac ggggatttcc atacggtgta caaactgcgc ttccgtggaa tgcgggttac
    12961 tgcgttggcg tatcgccatg gcgatcagtg ccaccgccgc accggacgag atgccggaga
    13021 agaggaacag caccggcagg atcgggttat taaggaacgg atacgatttc agcgccgaca
    13081 gcagaaaacc ggtataagcc cccaacaaca ccgccagcac cagcatcaat gtttccagtc
    13141 cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc ttcggcaacc
    13201 agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac agcaccagca
    13261 ccaccatgta aagctgaaac agcatcaccc ccatcgacat caccgaagta aaactgtagt
    13321 ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg ctcaacagac
    13381 cgaggatcac cgcgccaggt ccgacaatca gcgtggtgcg cagcaacgtg ctgtctgcac
    13441 cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga ccggcagaaa
    13501 taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat tcaaaatgaa
    13561 atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc ggtacagctt
    13621 cggtttggtt cccagcgcca gcttgtagcg gtaagtgggc ttctggcgca gcagttgcga
    13681 aatctcactg ttgggatcgt ccagattgcc aaacgtcagc gccttcgtcg ggcaagcctc
    13741 aacgcacgcg ggaagcttac cggcctgcaa attggtctta cggcagaaat cgcatttgtc
    13801 cgctgtcttc gtgaccggat gaataaagcg cacgcggtac ggacaggcgg cgatgcagta
    13861 ctgacaaccg acgcagagat ccggattaac atcgacaatc ccactggcag catcgcgaaa
    13921 agatgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct ggcaagactt
    13981 acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac gaataatcgt
    14041 caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca tacaggcggt
    14101 acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat tgatattcaa
    14161 cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc cgacgccggt
    14221 gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac agacgccggg
    14281 ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt aatccgtcct
    14341 ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca gctggcgcac
    14401 gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg ttccggcaga
    14461 tggcaggaca tacagacgct gttctgctct ccaaccttgt acatcggctc gttaaagcgc
    14521 atcacatctt tcaccccttc gcggtgttgc ggtgatggct ggccgtgaca gttggtgcag
    14581 gtgaccggca gtttgttatt cgggttgata acggaagcat gtttgccatg cataccttcg
    14641 gtatctggct tatgacagtc cagacaggcg gcatccgggt tacgctgctg ggtaacttcg
    14701 tagcgatcat cagatgcctg cgccgcaggg gtagcggtaa ttccgttcag gctccacaac
    14761 aggcccgacg ccagcacccc ggcagttaat aacgaacgta atacgctcat attcactcca
    14821 ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga ccgtttttac
    14881 gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc tcggccttga
    14941 tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag atatccggga
    15001 tctggatttc atgggtgatg cctttagtcg ccagcaggcg cgccagtttg gtgcgtgcat
    15061 ccgccgcttt atccatcgcc gtaccgagca tcctcagacc ttcttccggt gcgtgcatat
    15121 gaatgccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg atatcgtcct
    15181 gaatcggctt catttcggct tccgtcgcac ctgcgtccag cgccgctttc gcttcgaagt
    15241 gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgac tgcttacgtt
    15301 ccgcgaccac tttttgcaag gcagctttgt cctgggtatg gcagttcgca caagtctggg
    15361 cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct tcggcgttct
    15421 gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga atgcccgctg
    15481 tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac agggagttag
    15541 tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact ttcatgccgt
    15601 catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc acatggcact
    15661 gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt ttaccaatgg
    15721 cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg cctttggcga
    15781 actctggaga ggcggtgtta tggcaatcgg cacaacctaa gttgttgacg atttccgggc
    15841 cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg atcagacgcg
    15901 ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct tcagcgtttt
    15961 tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca tgtccacgcg
    16021 gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc cgtggatctt
    16081 ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag agatattgat
    16141 cgggatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt tttgcgggag
    16201 cagccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat aagctgaaga
    16261 tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg cgccattgtt
    16321 gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt catgacattg
    16381 ctcgccccta agtgtaacaa ataaccacac tgtgaatgtt gtctttaatc aattgtaagt
    16441 gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac agcatgcata
    16501 actgcatgtt cctcaaagaa ttaatcaact tttgttgctg accttcaaaa attaccctgc
    16561 cgtttatttg cacaattcta cttttgcgtg atctgtcgcc caaatactaa acaaaactgc
    16621 caatacccct acatttaacg cttatgccac atattattaa catcctacaa ggagaacaaa
    16681 agcatgagtc aaattcacaa acacaccatt cctgccaaca tcgcagaccg ttgcctgata
    16741 aaccctcagc agtacgaggc gatgtatcaa caatctatta acgcacctga taccttctgg
    16801 ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa aaacacctcc
    16861 tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa tctggcggca
    16921 aactgccttg accgccatct gcaagaaaac ggcgatcgta ccgccatcat ctgggaaggc
    16981 gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga cgtctgccgc
    17041 ttcgccaata ccctgctcaa gctgggcatt aaaaaaggtg atgtggtggc gatttatatg
    17101 ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgtattgg cgcggtgcat
    17161 tcggtaattt tcggtggctt ctcgccggaa gcggttgccg ggcgcattat cgattccaac
    17221 tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat tccgctgaag
    17281 aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca tgtggtggta
    17341 ctgaagcgta ctggcgggaa aattgactgg caggaagggc gcgacctgtg gtggcacgac
    17401 caggttgagc aagccagcga tcagcaccag gcggaagaga tgaacgccga agatccgctg
    17461 tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca cactaccggc
    17521 ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca tccgggcgat
    17581 atctactggt gcaccgccga tgtgggctgg gtgaccggac acagttattt gctgtacggc
    17641 ccgctggcct gcggcgcgac cacgctgatg tttgaaggcg taccgaactg gccgacgcct
    17701 gcccgtatgg cacaggtggt ggacaagcat caggtcaata ttctctatac cgcgcccacg
    17761 gcgattcgcg cgctgatggc ggaaggcgat aaagcgatcg aaggcaccga ccgttcgtcg
    17821 ctgcgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga gtggtactgg
    17881 aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac cgaaaccggc
    17941 ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc ggcaacacgt
    18001 ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct ggaaggggct
    18061 accgaaggta gcctggtgat caccgactcc tggccgggtc aggcgcgtac gctgtttggc
    18121 gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta tttcagcggc
    18181 gacggcgcgc gtcgtgatga agatagctat tactggatca ccgggcgtgt ggacgatgtg
    18241 ctgaacgtct ccggtcaccg tctgggaacg gcggagattg agtcggcgct ggtggcgcat
    18301 ccgaaaatcg ccgaagccgc tgtcgtcggt attccgcaca atattaaagg tcaggcgatc
    18361 tacgcctacg tcacgcttaa tcacggggag gaaccgtcac cagaactgta cgcagaagtc
    18421 cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct gcactggacc
    18481 gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg caaaattgcg
    18541 gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg cgtagtcgag
    18601 aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat tgccggatgc
    18661 gagtcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag gcattgattg
    18721 ccggatgcgg cgtataacgc cttatccggc ctacattcgg caagggttac ccgagcgtta
    18781 accttctccc ataagggagc gggaattaaa acaatcccta cattacctct ggagaatctg
    18841 tgatgaatgg cactatttat cagcggatag aagacaatgc gcatttcagg gagttagtcg
    18901 aaaaacggca acggtttgcc accatcctgt cgattattat gctggcagtt tatatcggct
    18961 ttattttact gatcgccttc gcgcccggct ggctgggcac cccgctgaat ccgaacacca
    19021 gcgtcacacg cggtattcca gtcggtgttg gggtaatagt gatctccttt gttctcactg
    19081 gtatctacat ctggcgggcg aacggcgaat tcgaccgtct taataatgaa gtcctgcatg
    19141 aggtacaagc atcatgaaaa gagttctgac ggcgcttgcc gccacactcc ctttcgcagc
    19201 taacgccgcg gatgctatta gcggggccgt agagcgccag ccaacgaact ggcaggcgat
    19261 tattatgttc ctgattttcg tcgtgtttac gctcggcatt acctactggg cgtcaaaacg
    19321 cgtacgttcg cgtaatgact attacaccgc aggcggcaat atcaccggtt ttcagaacgg
    19381 gctggcgatt gccggtgact atatgtccgc cgcctcattt ttggggatct ccgcactggt
    19441 gtttacctcc ggctatgacg gcttaattta ctcgctgggc ttcctggtgg gctggccgat
    19501 cattttgttc ctgattgccg aacgtctgcg taacctgggg cgttacacct ttgccgatgt
    19561 agcctcttat cgtctgaaac aagggccgat tcgtattctt tcggcctgtg gttctctggt
    19621 ggtggtggcg ctttacctta tcgcccagat ggtgggtgca ggtaaactga tcgagctgct
    19681 gtttggcctt aactatcaca ttgcagtggt gctggtcggc gtgctgatga tgatgtacgt
    19741 cctgttcggc ggcatgctgg cgaccacctg ggtacagatt atcaaagctg tgctgttgct
    19801 gttcggtgcc agctttatgg cctttatggt gatgaaacac gtcggcttta gcttcaacaa
    19861 tctgttcagc gaagcgatgg cggtacaccc gaaaggtgtc gacatcatga aaccgggcgg
    19921 actggtgaaa gatccgatct ccgcactctc tctggggcta ggactgatgt ttggtacggc
    19981 tggcttgccg cacattctga tgcgcttctt tacggtcagc gatgcccgcg aagcgcgtaa
    20041 aagcgtgttc tacgctaccg gatttatggg ctacttctat atcctgacct ttattatcgg
    20101 cttcggcgcg atcatgctgg tcggtgcgaa tccggaatat aaagacgcgg cgggccatct
    20161 gattggtggt aacaacatgg cagcagttca cctggccaat gcggtgggcg gcaacctgtt
    20221 cctcggcttt atttcagcgg ttgctttcgc cactatcctc gcggtggttg cgggtctgac
    20281 gctggcgggc gcatcagcgg tttcgcatga cctgtacgct aacgtcttca aaaaaggcgc
    20341 gaccgaacgt gaagagctgc gggtatcgaa aatcaccgtc ctgattttgg gtgtgattgc
    20401 gattatcctc ggcgtgctgt ttgagaatca gaacatcgcc tttatggtgg ggctggcgtt
    20461 tgccatcgcg gcgagctgta acttcccgat cattctgctt tctatgtact ggtcgaaact
    20521 gaccacgcgt ggcgcgatga tgggtggctg gctggggctg attaccgcag tggtactgat
    20581 gatcctcggc ccgacgattt gggtacagat cctcggtcac gaaaaagcca tcttcccgta
    20641 tgaatacccg gcgctgttct ctatcaccgt ggcattcctc ggcatctggt tcttctcggc
    20701 aaccgataac tcagcggaag gcgcgcgtga gcgtgaactg ttccgcgcgc agtttatccg
    20761 ctcccagacc ggctttggcg ttgagcaagg ccgcgcgcat taatctctcc ccttccccgg
    20821 tcgtctgacc ggggaatact cttcctctcc caaatgcatc accttttccc ataatattaa
    20881 acaaataaac tcattaaaaa aaatgagcga tttttgacag tcgtagaaaa tgataatgca
    20941 gagaatatgc cttttctttc ttgttaatta taaggatatt ttatgcgcta taatggttta
    21001 aataatatgt ttttccctct ttgcctgatt aacgataacc actctgtcac aagtctatca
    21061 catacaaaga aaacaaaatc agataattac agcaaacatc ataaaaacac gttaattgac
    21121 aataaagccc tctctctttt caaaatggat gatcatgaaa aagtgataga cttgattcag
    21181 aaaatgaaaa gaatttatga tagtttacca tcaggaaaaa tcacgaaaga aacggacagg
    21241 aaaatacata aatattttat agatatagct tcatatgcaa ataataaatg tgacgataga
    21301 attacgagaa gagtttacct taataaagat aaggaagtgt caattaaggt ggtatatttt
    21361 ataaataatg tcaccgtcca taataatact atcgaaatcc cacagacagt gaatggtggt
    21421 tacgattttt cacaccttag cctgaaaggt atcgtgatta aagatgaaga tttatccaat
    21481 tcgaattttg caggttgcag actacaaaac gctattttcc aggactgtaa tatgtataaa
    21541 acgaatttta atttcgccat aatggaaaaa atactttttg ataattgtat tctcgatgac
    21601 tcatatttcg ctcagataaa aatgactgac ggaactctaa attcatgctc cgctatgcat
    21661 gttcaattct acaatgcaac aatgaataga gccaatatta aaaatacctt ccttgattat
    21721 tcaaattttt atatggcata catggctgag gtaaatcttt ataaagtaat agcgccatat
    21781 attaatttat ttagagccga ccttagcttc tctaaacttg atttaattaa ctttaaacat
    21841 gctgatctgt ctcgcgtcaa tctgaacaaa gcaatcctcc agaatataaa cttaattgat
    21901 a
//
LOCUS       DAZFDC010000081        21467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFDC010000081 DAZFDC010000000
VERSION     DAZFDC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.081"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            53..1567
                     /gene="caiT"
                     /locus_tag="ACHJ48_003088"
     CDS             53..1567
                     /gene="caiT"
                     /locus_tag="ACHJ48_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO4708592.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFLWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1598..2740
                     /gene="caiA"
                     /locus_tag="ACHJ48_003089"
     CDS             1598..2740
                     /gene="caiA"
                     /locus_tag="ACHJ48_003089"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO4708593.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2869..4086
                     /gene="caiB"
                     /locus_tag="ACHJ48_003090"
     CDS             2869..4086
                     /gene="caiB"
                     /locus_tag="ACHJ48_003090"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO4708594.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4160..5713
                     /gene="caiC"
                     /locus_tag="ACHJ48_003091"
     CDS             4160..5713
                     /gene="caiC"
                     /locus_tag="ACHJ48_003091"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO4708595.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDTFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYDAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFIDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5822..6607
                     /gene="caiD"
                     /locus_tag="ACHJ48_003092"
     CDS             5822..6607
                     /gene="caiD"
                     /locus_tag="ACHJ48_003092"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO4708596.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEESYSYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6613..7203
                     /gene="caiE"
                     /locus_tag="ACHJ48_003093"
     CDS             6613..7203
                     /gene="caiE"
                     /locus_tag="ACHJ48_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO4708597.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYTDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7322..7717)
                     /gene="caiF"
                     /locus_tag="ACHJ48_003094"
     CDS             complement(7322..7717)
                     /gene="caiF"
                     /locus_tag="ACHJ48_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO4708598.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7978..11199)
                     /gene="carB"
                     /locus_tag="ACHJ48_003095"
     CDS             complement(7978..11199)
                     /gene="carB"
                     /locus_tag="ACHJ48_003095"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO4708599.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11217..12365)
                     /gene="carA"
                     /locus_tag="ACHJ48_003096"
     CDS             complement(11217..12365)
                     /gene="carA"
                     /locus_tag="ACHJ48_003096"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO4708600.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12821..13642)
                     /gene="dapB"
                     /locus_tag="ACHJ48_003097"
     CDS             complement(12821..13642)
                     /gene="dapB"
                     /locus_tag="ACHJ48_003097"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO4708601.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLDLNNL"
     gene            complement(13809..14723)
                     /gene="rihC"
                     /locus_tag="ACHJ48_003098"
     CDS             complement(13809..14723)
                     /gene="rihC"
                     /locus_tag="ACHJ48_003098"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO4708602.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(14789..15739)
                     /gene="ispH"
                     /locus_tag="ACHJ48_003099"
                     /gene_synonym="lytB"
     CDS             complement(14789..15739)
                     /gene="ispH"
                     /locus_tag="ACHJ48_003099"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO4708603.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15741..16190)
                     /gene="fkpB"
                     /locus_tag="ACHJ48_003100"
     CDS             complement(15741..16190)
                     /gene="fkpB"
                     /locus_tag="ACHJ48_003100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4708604.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16278..16772)
                     /gene="lspA"
                     /locus_tag="ACHJ48_003101"
     CDS             complement(16278..16772)
                     /gene="lspA"
                     /locus_tag="ACHJ48_003101"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO4708605.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVTLVVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16772..19588)
                     /gene="ileS"
                     /locus_tag="ACHJ48_003102"
     CDS             complement(16772..19588)
                     /gene="ileS"
                     /locus_tag="ACHJ48_003102"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO4708606.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPDEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFAHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYTEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19631..20572)
                     /gene="ribF"
                     /locus_tag="ACHJ48_003103"
     CDS             complement(19631..20572)
                     /gene="ribF"
                     /locus_tag="ACHJ48_003103"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO4708607.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20580..20804)
                     /gene="yaaY"
                     /locus_tag="ACHJ48_003104"
     CDS             complement(20580..20804)
                     /gene="yaaY"
                     /locus_tag="ACHJ48_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO4708608.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGSQFLPVICAMTMT
                     MKSHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20907..21170
                     /gene="rpsT"
                     /locus_tag="ACHJ48_003105"
     CDS             20907..21170
                     /gene="rpsT"
                     /locus_tag="ACHJ48_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO4708609.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5099 a         5762 c         5535 g         5071 t
ORIGIN      
        1 aaaacaaacg ggaacgcaaa ataaatattc gttttcacag tggaattaac tcatgaagaa
       61 tgaaaagaga aaaacgggaa tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg
      121 catactttgt tggcttacag tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt
      181 attcagttac gtcaccaatg tatggggatg ggcatttgaa tggtatatgg tggtgatgct
      241 tttcggttgg ttctggctgg tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc
      301 acctgaattt agtaccgcca gttggatctt tatgatgttc gcctcctgta cgtctgctgc
      361 cgtactgttc tggggatcga ttgagatcta ctactacatc tccaccccgc cgtttggctt
      421 agaaccgaac tcgacaggag cgaaagagtt ggggctggct tacagcttgt tcctctgggg
      481 acctctgccg tgggccactt acagcttcct ttcagtcgcc ttcgcttact tcttctttgt
      541 ccgcaaaatg gaagtgattc gccccagctc caccctggtg ccgctggtag gtgaaaaaca
      601 cgccaaaggg ttgttcggca ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc
      661 gatgggtacc agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt
      721 tggcattccg cataccctgc aactggacgc tatcatcatt acctgctgga ttatcctcaa
      781 cgccatttgc gtcgcctgcg gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag
      841 ttacctgagc ttcctgatgc tgggttgggt gttcatcgtc agcggtgcca gcttcatcat
      901 gaactacttc accgattcgg tggggatgtt gctgatgtat ctgccgcgca tgttgttcta
      961 taccgatccc atcgctaaag gcggcttccc gcagggctgg accgtgttct actgggcatg
     1021 gtgggtgatt tacgccatcc agatgagtat cttcctcgcc cgcatctccc gtggtcgtac
     1081 tgtgcgtgaa ctgtgcttcg gcatggtgat gggactgaca gcatccacct ggatcctgtg
     1141 gactgtactc ggtagtaaca ctctgctgtt gatagataaa aacatcatca acattccaaa
     1201 tctgatcgaa cagtacggtg tggcgcgcgc catcatcgaa acctgggccg ctctgccgct
     1261 cagcaccgcc accatgtggg gcttcttcat cctctgcttt attgccaccg ttacgctggt
     1321 taacgcctgc tcttataccc tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga
     1381 accaccgctg ctggtgcgta tcggttggtc aattctggtt ggcattatcg gtattgtttt
     1441 gctggcgctc ggcggcctga aaccgattca aactgccatt atcgccggag gatgcccgct
     1501 gttcttcgtc aacattatgg tgacgctctc ctttattaaa gacgcgaaac agaactggaa
     1561 agattaatta acccccaaaa tatcaagagg ttgaaagatg gattttaatt taaatgatga
     1621 gcaggaactg tttgtcgccg gtatccgcga actgatggcc agcgaaaact gggaggccta
     1681 ttttgccgag tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag cactggcgga
     1741 tatgggtatc gacagtctgc tgatccctga agagcacggt ggtctggacg cggggtttgt
     1801 tactctcgcc gccgtgtgga tggagctggg acgtctgggg gcaccaacct atgtactgta
     1861 ccagttgccg ggcgggttca ataccttcct gcgcgaaggc acacaagagc agatcgacaa
     1921 gattatggct ttccgcggca ccggtaagca gatgtggaac tcagcgatta ccgaaccggg
     1981 cgcgggctcc gacgtgggta gcctgaaaac gacttatacc cgtagaaatg gtaagattta
     2041 tcttaatggt agtaagtgtt ttattaccag tagcgcctac accccgtaca tcgtggtgat
     2101 ggcgcgcgac ggggcttctc cggacaaacc tgtctacacc gaatggtttg ttgatatgag
     2161 caaaccgggc atcaaagtga ccaaacttga gaagctcggt ctgcgtatgg atagctgctg
     2221 tgaaatcacc tttgacgatg tggaactgga cgagaaagac atgttcggtc gggaaggtaa
     2281 cggctttaac cgcgtcaaag aagagttcga ccatgaacgt ttcctggtag ccctgaccaa
     2341 ctacggtacg gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca
     2401 gtttggcgag gctattggtc gtttccagtt gattcaggaa aaattcgccc acatggcgat
     2461 caaattaaac tccatgaaaa acatgctgta tgaagcagcg tggaaagcag acaacggcac
     2521 catcacctct ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg catttgaagt
     2581 ggtggatagc gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag
     2641 ccgcttctgg cgtgacctgc gtgtagaccg cgtttccgga ggatctgacg aaatgcagat
     2701 cctgacgctg ggtcgtgcgg tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc
     2761 gacgctgacg cgtcttatca ggcctacggg agcacgaatg taggccggat aaggcgttta
     2821 cgccgcatcc ggcagtcatg cgctacataa atttttcagg agctaataat ggatcatcta
     2881 cccatgccga aattcgggcc gttggccgga ttgcgcgttg tcttctccgg tatcgaaatt
     2941 gccgggccgt tcgccgggca aatgttcgca gaatggggtg cggaagttat ctggatcgag
     3001 aacgtcgcct gggccgacac cattcgcgtt caaccgaact acccgcaact ctcccgccgc
     3061 aatttgcacg cgctgtcgtt aaatattttc aaagatgaag gccgcgaagc gtttctgaaa
     3121 ttaatggaaa ccaccgatat cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt
     3181 ggcattaccg atgaagtact gtggcaacac aacccgaaac tggttatcgc tcacctgtcc
     3241 ggttttggtc agtacggcac cgaggagtac accaatcttc cggcctataa caccatcgcc
     3301 caggccttca gtggttacct gattcagaac ggtgatgttg accagccaat gcctgccttc
     3361 ccgtataccg ccgattactt ttctggcctg accgccacca cggcggcgct ggcagcactg
     3421 cataaagcgc gtgaaaccgg taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg
     3481 ctgcgtatgg gccagtactt catgatggat tacttcaacg gcggcgaaat gtgcccgcgc
     3541 atgagcaaag gtaaagatcc ctactatgcc ggttgcggtc tgtataaatg tgccgacggc
     3601 tacatcgtga tggaactggt gggcattacc caaattgaag agtgctttaa agatattggc
     3661 ctcgcacatc tactcggtac gccggaaatc ccggaaggca ctcagcttat ccaccgtatc
     3721 gaatgccctt acggcccact ggtagaagaa aaactcgatg cctggctggc ggcacatacc
     3781 atcgcagaag ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc
     3841 gtaccggaac tggaaagcaa tccacagtat gttgcccgtg aatcaatcac tcagtggcaa
     3901 acgatggatg gtcgcacctg caaagggccg aacatcatgc cgaaattcaa aaataacccc
     3961 ggacaaatct ggcgcggaat gccctcacat ggcatggaca cggctgccat tttgaaaaat
     4021 atcggctaca gcgaaaacga cattcaggag ttggtcagca aaggtctggc caaagttgag
     4081 gactaaacgc gtggcgtcag tcggcttgcc tgactagcgc ccgcccaata acaaacgaac
     4141 aaaaatggat agaggtgcaa tggatatcat tggcggacaa catctacgtc aaatgtggga
     4201 cgatctggcg gacgtttacg gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt
     4261 cgttaaccgg tatagttatc ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt
     4321 ttatacgctg gggattcgca aaggcgacaa ggttgcacta catctcgaca actgcccgga
     4381 atttatcttt tgctggttcg ggctggcaaa aattggcgcg attatggtgc cgattaacgc
     4441 ccgcctgtta cgcgaggaaa gtgcgtggat cctgcaaaat agccaggcgt gcctgctggt
     4501 gaccagtgcg caattctatc ctatgtatca acagattcag caagaagatg ccactcaatt
     4561 gcggcacatt tgcctgacag atgtggcact tcccgctgat gatggcgtga gttcgtttac
     4621 tcaactgaaa aatcaacaac ctgccacctt gtgctatgca ccgccgctat cgactgacga
     4681 tacggcggaa attctcttca cctccggcac cacctcccga ccgaaaggtg tggtgattac
     4741 ccattacaac ctgcgcttcg ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga
     4801 tgacgtctac ctgacggtaa tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat
     4861 ggcggcgttt tctgccgggg ccacctttgt gctggtcgag aaatacagcg cccgcgcctt
     4921 ctggggacag gtacagaagt accgcgccac cattaccgaa tgtattccga tgatgattcg
     4981 tacgttgatg gtgcagccgc cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat
     5041 gttttatctc aacttgtcgg agcaggaaaa agacacattt tgtgaacgct tcggtgttcg
     5101 cttgctgacg tcttatggga tgacggaaac cattgtgggc attatcggcg atcgccctgg
     5161 cgataaacga cgctggccgt cgattggtcg ggcggggttt tgctacgacg cggagatccg
     5221 cgacgatcac aatcgcccgc tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt
     5281 accagggaaa accatcttca aagagtattt tctcaacccg aaagccactg caaaagtgct
     5341 ggaagctgat ggctggctgc ataccggcga taccggatac cgcgacgaag aaggcttttt
     5401 ttatttcatc gatcgccgct gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt
     5461 ggagctggaa aatatcatcg ccacccatcc aaaaattcag gatatcgtgg ttgtgggtat
     5521 taaagattcg attcgcgatg aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac
     5581 attgagcgaa gaggaatttt tccgcttctg cgaacaaaat atggcgaaat ttaaagtgcc
     5641 ctcttatctg gagatcagaa aagatctgcc acgtaattgc tcggggaaaa taattagaaa
     5701 gaatctgaaa taaatgaaac ggcagggaac taccctgcca gctaataatc acgctctaaa
     5761 acaatatgca ttttttgcag ggatgttgtc gtccctgaaa aagcaaaaat ggagaaaagg
     5821 aatgagtgaa tcattacatc tgacccgcaa tggatcaatt ctggaaatta ctcttgatcg
     5881 cccaaaagcg aatgctattg atgcaaaaac cagctttgaa atgggcgaag tatttctaaa
     5941 tttccgtgac gatccgcaat tacgtgtcgc cattattacc ggtgccggag agaaattctt
     6001 ttccgcgggc tgggatttaa aagcggcagc agaaggcgaa gcaccggatg ctgactttgg
     6061 cccgggtggt tttgcgggat taaccgaaat tttcaatctc gacaaaccgg ttatcgcagc
     6121 tgtgaacggc tacgcctttg gcggcggttt cgaactggcg ctggcggcag attttattgt
     6181 ttgtgccgat aacgccagct tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag
     6241 cggcggtgtg ctgcgtctgc cgaagatcct gccgcctgcc atcgtcaatg aaatggtgat
     6301 gaccggcaga cgaatgggcg cagaagaggc gctgcgttgg gggatagtca accgcgtggt
     6361 tagccaggcg gaactgatgg ataacgcccg cgaactggct cagcagctgg ttaacagcgc
     6421 cccgctggcg attgcggcgc tgaaagagat ctaccgcacc accagcgaaa tgccggtaga
     6481 agaatcgtat agctatattc gcagcggcgt gttgaaacac tatccatcgg ttctgcattc
     6541 ggaagatgcc attgaagggc cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg
     6601 acgttaacaa ccgtgagcta ttacgccttt gaggggttaa ttccggtggt tcacccgacg
     6661 gcgtttgtcc atcccagtgc cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac
     6721 atcggcccac tcgcctcact gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc
     6781 aatattcagg atggctgcat tatgcatggc tacaccgaca ctgacaccat cgttggggaa
     6841 aacggccata tcgggcacgg agcgatcctg catggttgtg tgattggtcg cgatgcattg
     6901 gtcgggatga acagcgtgat tatggatggc gcggtcattg gcgaagagag cattgttgcc
     6961 gccatgagct ttgtcaaagc aggctttcac ggcgagaaac gccagttgtt gatgggtacg
     7021 ccagcccgcg ctgtacgcag tgttagtgac gacgagttac actggaaacg gttgaatacc
     7081 aaagagtatc aggatcttgt cgggcgctgt catgcatcgt tacatgaaac acagccactg
     7141 aggcaaatgg aggaaaatcg cccccgtttg caggggacga cggatgtgac gcctaaacgg
     7201 tgatcttgcc ggatgcggcg taaacgcctt atccaactct gctggagagc aaccgtaggc
     7261 cggataagat gcgccagcat cgcatccggc gaccaggtcg cacggtatca tcgaaatgac
     7321 attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg ttaagttccg
     7381 tgagcggaac ggcaggaata cgcggtgtct ttcttacgcc gactaatttt tcccgactgt
     7441 tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cactttaacc agtcgctgac
     7501 actggcatcc ccgcccttcc agcttattag ggatcatctt aacttcgcag cttatttctg
     7561 tgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca atatcgaaat
     7621 gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat tcggcgatta
     7681 acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt tcacaaatta
     7741 aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa attgtggata
     7801 tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct tatgtcatca
     7861 aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaacggtcc tcatcagaga
     7921 accgtattag ttattcgatc aaattagcgg atgaaaaata tccgccatga cacgctatta
     7981 tttgatctgt gcgtgcattt cctgcaccga aattactttt tcagtcgcat cggcattcag
     8041 cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca taatgcactt tatattgcag
     8101 cgcactgcga cggatcacgc gggagtcttc aatcgcacga cggcctgagg tggtgttgat
     8161 gatgtaggta tattcgccat tcttgatacg gtcctgaatg tgcggacggc cttcatgcac
     8221 cttgtttacc agacgcggat tgatacccgc ttcgcccagc acaatcgccg tgccgtgggt
     8281 cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca cgcgttcttt
     8341 atcgccttcg cgcacggaaa gcagcgcacg accgtgtttc ttcatggtgg agttgctgcc
     8401 cagctgcgct ttggcaaacg cttcagcgaa ggtgcggccc acgcccatga cttccccggt
     8461 agaacgcatt tctggcccta acagcgggtc aacgcccggg aatttattga acggcagcac
     8521 cacttctttc accgagtagt acggcgggat aacttctttg gttacgccct gctcagccag
     8581 cgatttgcca gccatcacac gcgccgccac ttttgccagc ggtacgccgg tggctttgga
     8641 gacgaacgga acggtacgcg ccgcacgcgg gttaacttca atcaggtaga cttcgttgtt
     8701 tttcaccgca aactgcacgt tcatcaggcc gcgcacctgc aattcgaagg ccagtttctg
     8761 cacctgctgg cgcattacat cctgaatttc ctgacttaag gtgtacgctg gcagcgaaca
     8821 tgcggagtca ccggagtgca cgcccgcctg ctcgatgtgc tccatgatgc cgccaatcag
     8881 caccatttcg ccgtcgcaga tggcatccac gtcaacttct actgcgtcat caaggaaatg
     8941 atccagcaac actggcgcat cgttagacac gctaaccgcc gtctggaagt agcgacgcag
     9001 gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag acggacgcac
     9061 caccagcggg tagccaatct cttttgcctt ctcaactgcc atttcaatag cagtaacggt
     9121 ggcgttcgcc ggttgtttca gtttcagacg gtcaaccgca tgctggaagc gttcacggtc
     9181 ttccgcacgg tcgatagcat ccgggctggt gccgataacc ggtacgccag cggcttccag
     9241 tgcacgcgcc agtttcagcg gggtctgacc gccgtactga acgataacgc ctttcggctt
     9301 ctcaatacgc acgatttcca gcacatcttc cagagttacc ggctcgaagt agaggcgatc
     9361 ggaagtgtcg tagtcggtag agacggtttc cgggttacag ttaaccataa tggtttcgta
     9421 accgtcttca cgcagcgcca gcgaggcgtg tacgcagcag tagtcgaatt cgataccctg
     9481 accgatacgg tttggaccgc cgccaagcac catgattttt tcacggtcgg tagacggatt
     9541 cgcttcgcac tcttcttcat aagtggagta catgtaagcg gtgtcggtgg caaactctgc
     9601 cgcacaggta tccacgcgct tgtagaccgg gtgcaggtca tattggtcac gcagcttacg
     9661 gatttccgct tcgcgtacgc ccgccagttt tgccaagcgc gcatcggcaa agcctttgcg
     9721 tttcagctgg cgcaggaatt cagcgttcag gccagtgatg cccacttccg ccactttctc
     9781 ttccagacgc accagttctt caatctgtac caggaaccag cggtcaatgt tagtcaggtt
     9841 gaagacgccg tccacagaca ggcccgcgcg gaaagcatcg gcgatgtacc agatacgctc
     9901 tgcgcctgcg tctttcagtt cgcgacggat tttggttaac gcttccgggt catccaggct
     9961 cactttcggg tcgaatccag tcgcaccgac ttccaggccg cgcagcgctt tttgcaggga
    10021 ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc gatttcatct gagtggtcag
    10081 acggtcgtta gcaccggcga atttttcgaa gttgaagcga ggaattttgg taaccacgta
    10141 atcgatggac ggctcgaagg aggccggagt acgtccgcca gtgatgtcgt tcatcagttc
    10201 atcgagggtg taacccaccg ccagtttcgc cgccacttta gcaatcggga aaccggtcgc
    10261 tttcgacgcc agcgccgaag aacgggacac gcgtgggttc atttcgataa caatcaggcg
    10321 accgtttttc gggttcaccg caaactggac attggaacca ccggtttcaa cgccgatttc
    10381 acgcagcacc gccatcgagg cgttacgcat gatttgatat tctttgtcgg tcagcgtttg
    10441 ggctggcgcg acagtgatgg agtcaccggt gtggatgccc atcgcatcaa agttttcgat
    10501 agagcagacg atgatgcagt tgtcgttttt atcgcgcacc acttccatct cgtactcttt
    10561 ccagccgatc agcgactcat caatcagcaa ctctttggtc ggagagagat ccagaccgcg
    10621 ggcgcaaatt tcttcaaact cttcgcggtt ataagcgata ccgccgccgc taccgcccat
    10681 ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca gcggcaaccg ccagcgcttc
    10741 ttccatcgta tgtgcgatac cggaacgcgc ggtttccaga ccaattttct tcatcgctac
    10801 gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg gcagtggcac caatcatggt
    10861 gacgccgaac tcttccaaca cgccctgacg ttccagctcc agcgcgcagt tcagcgccgt
    10921 ctgaccgccc atggttggca gcaccgcgtc cgggcgctct ttttcaataa tcttacgtac
    10981 cacttcccag tgaatcggct cgatgtaggt cgcatcggcc atttccgggt cggtcatgat
    11041 ggtcgccggg ttggagttca ccagaatgac gcggtaaccc tcttcgcgca gggctttaca
    11101 tgcttgcgcg ccagagtagt caaactcaca cgcctgaccg ataacaatcg ggcccgcacc
    11161 cagaatcagg atacttttta tatctgtacg ttttggcatg gctcttttac tcctgattac
    11221 ttagcggttt tacggtactg ctcaattaac tcgataaagt ggtcgaacaa cggcgcggcg
    11281 tcgtgtggac cagggctggc ttcagggtga ccctggaagc tgaacgccgg tttatcggtg
    11341 cgatgaatgc cctgtaacgt accgtcgaac agggatttat gcgtgacacg caggtttgca
    11401 ggtaatgttg cttcatccac cgcaaaaccg tggttctggg cggtgatcat caccacgttt
    11461 ttctccacat ctttaaccgg atggttgccg ccgtggtgac caaatttcat tttgacagtc
    11521 ttcgcaccgc tcgccagcgc cagcagctga tgaccgaggc agatgccgaa gaccggaata
    11581 tcggtttcga ggaatttctg gatggcggta atggcgtaat cgcacggggc cgggtcgcca
    11641 ggaccgttgg agaggaagat gccgtctgga ttcattttca gcacatcttc cgcagaagtt
    11701 tgcgccggaa cgatggtcag gcgacagcct ctatccacca gcatccgcag gatgttgcgt
    11761 ttggcaccaa aatcataagc cacgacgtgg aacggcagct cgtcttcttt tttcgcttct
    11821 ggcaggccgc cggtcaacgt ccagctccct tgtgtccagc tataggcttc tgcggtggtc
    11881 acttcttttg ccagatccat gccattcaga cctgggaacg cgcgggcttt ttctaacgcc
    11941 agcgccgcat ccgggttatc gcctgcgata atgcagccat tctgtgcgcc tttctcgcgc
    12001 agtaaacgcg tcagcttacg ggtatcgata tcggcaatcg ccacgatgtt atggcgcttc
    12061 aggtaagaag agaggtcttc ggtattacgg aagttgctgg caatcagcgg caggtcgcga
    12121 atcaccagac cttgtgcatg tacctgagaa gattcttcat cggcgtcatt agtgccgaca
    12181 ttgccaatat ggggataagt aagagtaacg atttgacgag aataggaagg atcagtgagg
    12241 atttcttgat aaccggtcat tgaagtattg aaaacgactt ccccaaccgc cgaacctgtt
    12301 gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca gaaccaatag cgctgactta
    12361 atcaaaacac cctccagaga atattcactc actttatttg catattaatt cacaatgatg
    12421 acgtaaatca atgcaaatct gcttaccgac gaatttctgg caaacggcgg cattctggag
    12481 atatgggcgc taaaagtcaa cttaatggca ggatttttta tattattttg tggctcatgc
    12541 atgttttaca ggcattaagc gacaaaaaga tcatctaacc tgttctggaa aacgcttgcg
    12601 cagcaagaag attgcatgaa ataaaaacga gcaggcaaaa gtggaccaaa tggtcaaaat
    12661 ttacattaag acaacaaaaa tagaagacaa aacatgtaat ttttggcagt tatagaaaaa
    12721 ttaaaagcat aacaccaaaa ataaaagggc taaaaattaa agccctttat aattaataaa
    12781 ttaaatatgt tacttttgca tcatcataaa cattttgtgg ttacaaatta ttgagatcaa
    12841 gcacatctcg catatcaaaa agaccatttt ccttaccact caaccacaaa gccgatctta
    12901 ccgcaccgtt agcaaatgtc atgcggctgg acgccttatg ggtgatctcc agacgctcgc
    12961 caatatcggc aaacatcgcg gtatgttcgc caacgatgtc acctgcacgc acggtggcaa
    13021 aaccaatggt accaggcaca cgttcaccgg tatggccttc acgactgtag accgcgcaat
    13081 ctttcagatc tttatcaagg gcgtgggcga tcgcctctcc cattgccagt gcggtgcctg
    13141 acggcgcatc aactttatgt ctatgatgtg cttcaataat ttcaatatcg gtgtagtcac
    13201 ccatcacttt ggctgctttc tccagcagct taagcatgac gttaacgcca acgctaaaat
    13261 tggcggcaaa gacaatcgca atatcggcgg cggcgtcacg aattgcctgt tttccggctt
    13321 cgtcaaaccc cgtagtgccg atcaccatcc ctttgccatg ctggcgacaa aaagcgagat
    13381 ggttcagcgt accttccgga cgggtaaaat cgataaacac atcaaaatca tcttttatcg
    13441 catcaagact gctttgcacg gtaacgcctg ttttccctgc accagccagc tcaccggcgt
    13501 cgctgcccag taaagaagat ccttcgcgct ccagcgccgc gcccagctgc acgccttcta
    13561 atgccagtgc cgcctgaatc aactggcggc ccatacgccc cccggctccc gcgatggcaa
    13621 cgcggatgtt tgcatcatgc atagctattc tcttttgtta atttgcatag accgttttca
    13681 gagtaaccag cgcatcgcta cgccgccagc caaaaacgct gataattaga aattaatgat
    13741 aaacagggag ggatatcagt gaaaggcatg agtcgatgaa tgactgcatg ccgataacat
    13801 gtgacaggtt acggcaccag agccagcacc tcagccaccc actgttggaa gccttttaca
    13861 ttcagatcca atgccacctg tacattggct ggtttgccca ggcaaccgtc gatatcaacc
    13921 accgtcgtgc ctgaggtaaa ttcgccttga gtttccactg ccacaaaaca gggtttgaga
    13981 gtgaacaggt ccgggcgcac cagccaggcg atggcgcaga gatcgtgcat tcgcaagccg
    14041 ctttgcatac tgccgctacg gtagtggcta aacagggcgt gaagcatttt cccggtacgg
    14101 tttaactgcg gcagtgtaga gagatagtca ggagttaata ttgcctgatt ggtgacatcc
    14161 aaaccgcaca tgacgatttc aataccactg cggaagacac aggcagcagc ttctggatcg
    14221 gcagcaatat taaactcggc gtttggcgta cagttgccgc gtccggcaga accacccatg
    14281 atcaccagac ggcgaatata cggtttacat tccgggcatt gtgaaagtaa cagcgcaata
    14341 ttggttaacg ggccgatggc caccagggta acgggctctg gtgcacgcat cagggcatcc
    14401 cgaatcgcca gaaacgccgg tatcccgagt ggctttcggt tgtgctcaac aaagtcgtag
    14461 ccagccattc ccgattcgcc gtgcacagat gctgcatcac gcggtgcgcg taccagcggc
    14521 acagcggctc cctgggcgag cggaatctcc gcattccaga aatgcagcag ttgcaaggcg
    14581 ttacgggtag ttttctcaac cgagacatta cccgcgacgg tggtcatcag ttgcaggtcg
    14641 agttcggggg caaaaatcgc ggcggcaatg gcgacggcat cgtcaatgcc ggggtcagta
    14701 tcgaggaaga taggtaagcg catgttttct ccataaaaaa tgccgataac aagaccggca
    14761 ttttcgcata acttaggctg ctaatgactt aatcgacttc acgaatatcg acacgcagct
    14821 ctttcggcac ttcgaaaaca atattttctt cacggccttc cagcggaatg gcttcaccac
    14881 cacccagctg ctgcaaacgt gccaccacat tctgcaccag aatatccgga gccgatgcgc
    14941 ccgcagtcac gccgacgcat ttaacctctt tcacccactc ttcctggata tctttcgcat
    15001 cgtcaatcaa aaacgcgcgt ttgcccatac gctgggccag ctccgccaga cggttggagt
    15061 tggaggagtt tttcgaaccg accaccaaca caacttccgc ctgttctgcc agggcgcgta
    15121 ccgcttcctg acggttagtc gtggcgtagc agatgtcatc tttgcgcgga ccgacaattt
    15181 tcgggaagcg tttacgcagc gcgtcgatca catcagacgt gtcatctacc gacagcgtgg
    15241 tttgggtcat aaaggagagc ttctcttcgt ttttgaccgt cagtttccac acatcgtcag
    15301 gcgattcgac cagatacatt cccccttcag ggttgctgta ctgccccatc gtcccttcca
    15361 cttccgggtg cccggcgtga ccgatgagaa tagactcttc gccacgacgg ctggcgcggg
    15421 cgacttccat atgcactttg gtcaccagcg gacaggtggc gtcgaatacc gtcaaatcac
    15481 ggcttttcgc ttcgttacgt accgcctgag aaacaccatg tgcggagaag atcaggatcg
    15541 cgccgtccgg cacttcgctg atctgctcaa taaagatagc tccacgctcg cgcaggctat
    15601 cgaccacgta gcggttatgc accacttcgt gacggacata tatcggtgcg ccgtaaatgg
    15661 caagcgcgtt ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa ccacgtgggt
    15721 tggccaacag gatctgcatg ttacgcctcc agtgccggat cgatttccag cacttcaata
    15781 tcaaaatgaa cggtctgccc ggccagcgga tggttgaaat cgacggtaat ggagtcgccg
    15841 ttaatttcgc ggatcacgcc aggcatctca ctgccatcca ttgcggtaaa aagcattatt
    15901 gcgccaattt ctggctcgcc tgcatccata aactcacggc gggagaagta ctgaatcagg
    15961 tccggtgacg gcacgccaaa cgccgcatct ggctccagcg agaaagtggt tttatcgccc
    16021 actttcagcc ccagcaggtg ttgctccagc ccttcggaaa gagaagcatc gcccaggcgg
    16081 aacagcgccg gtttaccgtt gttgcgggta gactcggcgg tggtgccatc gtcgagtttt
    16141 agcgtgaagt gcaccaggac ggcgctattg ctctgtacag attcagacat gcaggtttgc
    16201 tcttatattt tgaggatttt tgccggatgc ggcctacgat ttcgcagcaa tctgtaggcc
    16261 ttatccggca gggtttatta ttgttttttc gctttagaag gcaaaaaacc ttccagcaca
    16321 atcagtgccg caccaacaca gatggcagta tcggcgaggt tgaaggtggc gaagtgccag
    16381 tcgccgacgt agaagtcgat catatcgaca acgaagccgt gccacaggcg gtcgaacagg
    16441 ttgcccagcg cgccgccaat aatcagcgcg taggcgatat tgtttagctt ctgcgtggcc
    16501 ttcgagcgat acatcatcac aaccagggta acgctaatac caatcgcaat accggcaaag
    16561 aaccaacgct gccagccgcc gctatcggca aggaaactaa acgccgcgcc atagttacgc
    16621 gcataatgca gattaagcga cgggaacagc gggaccgtat cccccagagc aaagttctgg
    16681 aggatcaggt atttgctgcc cagatcgata atcagcacga ctaccaccag ccacagccag
    16741 cgtagccctg ttgaacagat cgattgactc atcaggcaaa cttacgtttt tcaccgtcac
    16801 cggcgacgtt gctgacacag cggccgcaga tttctgcgtg ttccgccacc ttgccgacat
    16861 cctgggtgta gtgccagcag cgtgggcact tctcaccttc ggctttactc aacgcgactt
    16921 tcagcccttt gagcacttcg ctctgctgag catcagcagg tgcgtcgtta tagtctgcaa
    16981 cggtagcgcc ggaggtcaac aggacaaatc gtaattcatc gcccaatgcg gtcagtttcg
    17041 ccgccagttc cggttctgta tacaaggtta ctgccgcttc cagcgagcca cccactttct
    17101 tgtcggcacg cgcctgctca atgactttgt tcacttcgcc acgcactttc aacagctcgt
    17161 cccagaacgc atcgttcatt gcttcactgt ctgccagacc aaacagacct tcgtaccact
    17221 cgccggtgaa gacgtatttt tcacgttcgc ccggcaggta gccccacact tcatcagcgg
    17281 tgaaggagag gattggtgcc atccagcgaa ccagcgcttc tgcgatgtga tacagcgcag
    17341 tctggcagct acgacgcgcc acgctgtccg ctttggcggt gtactgacgg tctttgatga
    17401 tgtcgaggta gaaggaaccc atctcaacgg agcagaagcg catcagacgc tgtaccactt
    17461 cgtggaaatc gtatgcttcg tatgccttga ggatgtcttc ctgtgccgct ttcgcacaac
    17521 ctacggccca gcgatccagt actaccatct cttccggttt caccatatct tttgccggat
    17581 caaaaccgtt caggtttgcc agcaggaagc gcgcggtgtt acggatacga cgatagctgt
    17641 cggcagcacg tttcaggatc tcgtcagaaa cggccatttc accggtgtag tcggttgatg
    17701 ccacccacag acgcagaata tccgcgccca gtttgttcat cacatcctgc ggcgacacgg
    17761 tgttgccgat ggatttagac atcttgcggc cctgaccatc tacggtaaag ccgtgggtca
    17821 gcacctgacg atacggcgct ttgcccttca tcgcggtgga gatcatcaga gaagacatga
    17881 accagccacg gtgttggtca gaaccttcca gatacatgtc cgcagcgtga ccggcaaatt
    17941 ccgggcgcac gtcaacaaca gaagagtgag tagagccgga gtcaaaccat acatccagcg
    18001 tatccggcac tttcacatac tgatcagctt catcgccgag gatctctttc gcatcaagat
    18061 cccaccacgc ctggatgcca tcaacttcaa cgcgtttagc cacttcttcc atcagttcga
    18121 gggtacgcgg atgcagctct tccgtgtctt tgtgcacgaa cagagacatc ggtacgcccc
    18181 aggtacgctg acgggagata caccagtcag gacggttagc gaccatcgac tcgatacgcg
    18241 cctggcccca gtccgggatc cactgcacac ctttgatctc tttcagagac tgcgcgcgca
    18301 gacctttctg atccatactg acgaaccact gcggcgttgc gcggaagatg atcggcgttt
    18361 tgtgacgcca gcagcacgga tagctgtgct gcattttctc aacgtgcagc agcgcgcctt
    18421 tttcctgcag cagcgcaacg acgatgtcgt tcgctttgaa gacgttcacg ccatccagcg
    18481 tcggataagt gcccggcaga taagtgccgt ccgggccaac cgggttagcg gtttccaggc
    18541 cgtatttctg accgatcaca tagtcgtccg ggccgtggcc aggcgcggtg tgaacggcac
    18601 cggtaccggc atccagggta acgtgatcgc cgaggattgc cgggacgtcg aagcccataa
    18661 acggatgggc aaagcgcagc agctcaagct ccgcaccttt taccgtgccg agaatggtgt
    18721 aatcggtcac gccgatacgc tgcattacgc tttcaaccag atctttcgcc agaatcacgg
    18781 cctgaccgtc gatctgcacc agcgcatagt cgaaatcagg tgcaatagag attgcgcggt
    18841 tcgcaggcag agtccacggc gtagtggtcc agatcaccag cgagattggg ccgttaacgt
    18901 tgcttacgcc aaatttcgct ttcagcgcat cctgatcgac cgcctggaaa gcgacgtcaa
    18961 tggacggaga agttttgtcg taatactcaa cttccgcttc tgccagtgca gaacggcagt
    19021 ctacgcacca gtgcaccggc ttcgcgcctt tgtgcaggtg accattgccg atgattttgc
    19081 ccagcgcgcg gatgatattg gcttcagttt tgaagtccat ggtcaggtac gggtgcgacc
    19141 agtcgcccag cacgcccaga cggataaagt ctttgcgttg accgtcaacc tgggtcgccg
    19201 cgtattcgcg gcacttggcg cggaactcgg cggcggtgaa tttctcatcc ggcttaccgt
    19261 attcttgctc tactttcagc tcgatcggca gaccgtggca gtcccagcca ggcacatacg
    19321 gcgagtcata accggaaagc cctttggact tcacgataat gtctttcaga atcttgttaa
    19381 ccgagtgacc aatatgaatg ctgccattcg cataaggagg gccatcatgc agaatgaagg
    19441 tttttttgcc ttttttagcc gcacgaatga tgccgtacag atcatcatca gtccaacgcg
    19501 ccagcattcc cggttcgcgc ttggcgagat cgccacgcat cgggaaccct gtttccggca
    19561 aattcagggt tgatttatag tcactcatca gattctcggt tccgtgtttc ggtttgatta
    19621 cataacaggc ttaagccggt tttgttagcc caaaaaattc gcgggcggtt aattcatcac
    19681 gcgcaatctg cgctttcagt tcgtccagcg aagcaaatcg ctgctcattg cgtatttttt
    19741 tacgcagcac tacttgtata tggcgaccgt aaaggtccat tgcaacatct aacaaatgca
    19801 cttccagctg ctggcgaata ccggcaaccg ttgggcgtgt tccgatgttt gccacgccgg
    19861 gtaacggctt ttcaccgagg cccagcactt ctaccgcata aacccctttc accggggaaa
    19921 cctgacggcg tagcggtaca ttcgccgtcg ggaaacctat agtgcgccct aattcatcac
    19981 cgtggactac acgcccggag atggcaaacg ggtgccccag taaactttct gccagagcca
    20041 aattgtcatc cgcaagggcc tgacgcacgg cggtgctgct gatacgcacg ccaccttcgc
    20101 aaaaagtttg cgtactggtg atatcgaagc cgtattccat gccagctttc tgtaataaca
    20161 agaaatcgcc ttcccgacca gcgccaaagc ggaaatcatc ccccacggcg aggaatttta
    20221 cccgcaaatg cttcaccaga agatcgctga tgaaattttg cgcggttaac gccgcgaaac
    20281 gcctgtcgaa acgtacgcac agcacgtaat caacgccaca ctctgcaagg taacgtagtt
    20341 tttcccgcag tcgggtcagc cgtgccgggg ctttatcggt agcaaacagt tccagtggtt
    20401 gaggttcaaa aagcatcacc atcaccggta agttgcgctt gcgcccttct tcctgcaagc
    20461 cctgtaacag cgcgcgatga ccgcgatgca cgccgtcgaa attaccaata gtcagcacac
    20521 acccttcttg cggggcctgg ctgagattat gtatgccgcg tatcagcttc atgtctggct
    20581 caaaacagtg aaaatcgtcc gagtatacct tgtacagcgg tcaaggttaa ccggcgattg
    20641 agtaccgaaa tcagaaagaa gtggcttttc attgtcatgg tcatggcgca aatcacggga
    20701 agaaactgac tgcctgctgc aatttttatc gcggaaaagc tgtattcaca ccccgcaagc
    20761 tggtagaatc ctgcgccatc actacgtaac gagtgccggc acattaacgg cgcttatttg
    20821 cacaaatcca ttgacaaaag aaggctaaaa gggcatattc ctcggccttt gaattgtcca
    20881 tatagaacac atttgggagt tggaccttgg ctaatatcaa atcagctaag aagcgcgcca
    20941 ttcagtctga aaaggctcgt aagcacaacg caagccgtcg ctctatgatg cgtactttca
    21001 tcaagaaagt atacgcagct atcgaagctg gcgacaaagc tgctgcacag aaagcattta
    21061 acgaaatgca accgatcgtg gaccgtcagg ctgctaaagg tctgatccac aaaaacaaag
    21121 ctgcacgtca taaggctaac ctgactgcac agatcaacaa actggcttaa ttgccaattt
    21181 gctgaagctt tgtgaaaaag cccgcgcaag cgggtttttt tatgcctgct gcttttgtga
    21241 tgcgtgagag aacacactgc cggttgccag atagtcaaaa gagacatgcc gttgcactat
    21301 ctgaactctt cgtggcttaa gaatcgtaga accatcggct gatacattct gaacaagaat
    21361 atctactcaa tctccagact cccctacaaa gaatgtggat attgtgatac acattgaggt
    21421 attaaactta agaccactct atttttgaat taaattccta tatactt
//
LOCUS       DAZFDC010000082        20993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFDC010000082 DAZFDC010000000
VERSION     DAZFDC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.082"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..474)
                     /locus_tag="ACHJ48_003106"
     CDS             complement(<1..474)
                     /locus_tag="ACHJ48_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4708610.1"
                     /translation="MKTWGCGGLELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKP
                     HERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAV
                     DANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLR"
     gene            complement(416..610)
                     /locus_tag="ACHJ48_003107"
     CDS             complement(416..610)
                     /locus_tag="ACHJ48_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708611.1"
                     /translation="MRALCAVNGSHPENLRFWLKACPVVLRERFHQQCKCRFNWSTQQ
                     LCENMGLRRFGTMEERNRLQ"
     gene            complement(631..912)
                     /locus_tag="ACHJ48_003108"
     CDS             complement(631..912)
                     /locus_tag="ACHJ48_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708612.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
     gene            complement(1450..1536)
                     /locus_tag="ACHJ48_003109"
     tRNA            complement(1450..1536)
                     /locus_tag="ACHJ48_003109"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1500..1502),aa:Leu,seq:taa)
     gene            complement(1549..1622)
                     /locus_tag="ACHJ48_003110"
     tRNA            complement(1549..1622)
                     /locus_tag="ACHJ48_003110"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1588..1590),aa:Cys,seq:gca)
     gene            complement(1676..1751)
                     /locus_tag="ACHJ48_003111"
     tRNA            complement(1676..1751)
                     /locus_tag="ACHJ48_003111"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1716..1718),aa:Gly,seq:gcc)
     gene            complement(1903..2451)
                     /gene="pgsA"
                     /locus_tag="ACHJ48_003112"
     CDS             complement(1903..2451)
                     /gene="pgsA"
                     /locus_tag="ACHJ48_003112"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO4708613.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(2508..4340)
                     /gene="uvrC"
                     /locus_tag="ACHJ48_003113"
     CDS             complement(2508..4340)
                     /gene="uvrC"
                     /locus_tag="ACHJ48_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO4708614.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(4337..4993)
                     /gene="uvrY"
                     /locus_tag="ACHJ48_003114"
     CDS             complement(4337..4993)
                     /gene="uvrY"
                     /locus_tag="ACHJ48_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO4708615.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            5135..5251
                     /locus_tag="ACHJ48_003115"
     CDS             5135..5251
                     /locus_tag="ACHJ48_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4708616.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            5289..5465
                     /gene="yecU"
                     /locus_tag="ACHJ48_003116"
     CDS             5289..5465
                     /gene="yecU"
                     /locus_tag="ACHJ48_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO4708617.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            5452..5676
                     /gene="yecF"
                     /locus_tag="ACHJ48_003117"
     CDS             5452..5676
                     /gene="yecF"
                     /locus_tag="ACHJ48_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO4708618.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEQQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(5744..6466)
                     /gene="sdiA"
                     /locus_tag="ACHJ48_003118"
     CDS             complement(5744..6466)
                     /gene="sdiA"
                     /locus_tag="ACHJ48_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO4708619.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(6696..7448)
                     /gene="tcyN"
                     /locus_tag="ACHJ48_003119"
     CDS             complement(6696..7448)
                     /gene="tcyN"
                     /locus_tag="ACHJ48_003119"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO4708620.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARKLLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(7445..8113)
                     /gene="tcyL"
                     /locus_tag="ACHJ48_003120"
     CDS             complement(7445..8113)
                     /gene="tcyL"
                     /locus_tag="ACHJ48_003120"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO4708621.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     TAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(8128..9114)
                     /gene="dcyD"
                     /locus_tag="ACHJ48_003121"
     CDS             complement(8128..9114)
                     /gene="dcyD"
                     /locus_tag="ACHJ48_003121"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO4708622.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASVEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(9219..10019)
                     /gene="tcyJ"
                     /locus_tag="ACHJ48_003122"
     CDS             complement(9219..10019)
                     /gene="tcyJ"
                     /locus_tag="ACHJ48_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4708623.1"
                     /translation="MKLAHLGRQALMGVMAVALIAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(10107..10658)
                     /gene="fliZ"
                     /locus_tag="ACHJ48_003123"
     CDS             complement(10107..10658)
                     /gene="fliZ"
                     /locus_tag="ACHJ48_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO4708624.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(10704..11423)
                     /gene="fliA"
                     /locus_tag="ACHJ48_003124"
     CDS             complement(10704..11423)
                     /gene="fliA"
                     /locus_tag="ACHJ48_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO4708625.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(11743..13500)
                     /locus_tag="ACHJ48_003125"
     CDS             complement(11743..13500)
                     /locus_tag="ACHJ48_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO4708626.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQAADSASKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTTPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            13766..15163
                     /gene="fliD"
                     /locus_tag="ACHJ48_003126"
     CDS             13766..15163
                     /gene="fliD"
                     /locus_tag="ACHJ48_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO4708627.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSSTTAFSATTAGNAIAGKYTISVTHLAQA
                     QTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNAKAG
                     VSASIINVGNGDYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEVSVA
                     AQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIKDWV
                     NAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVSSSS
                     YKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGSNLT
                     SWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSLNST
                     SSYLTQQFENNSNSK"
     gene            15188..15598
                     /gene="fliS"
                     /locus_tag="ACHJ48_003127"
     CDS             15188..15598
                     /gene="fliS"
                     /locus_tag="ACHJ48_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO4708628.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            15598..15963
                     /gene="fliT"
                     /locus_tag="ACHJ48_003128"
     CDS             15598..15963
                     /gene="fliT"
                     /locus_tag="ACHJ48_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO4708629.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            16041..17528
                     /gene="amyA"
                     /locus_tag="ACHJ48_003129"
     CDS             16041..17528
                     /gene="amyA"
                     /locus_tag="ACHJ48_003129"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO4708630.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPNEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRNYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVL"
     gene            complement(17562..17975)
                     /gene="yedD"
                     /locus_tag="ACHJ48_003130"
     CDS             complement(17562..17975)
                     /gene="yedD"
                     /locus_tag="ACHJ48_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO4708631.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            18162..19367
                     /gene="yedE"
                     /locus_tag="ACHJ48_003131"
     CDS             18162..19367
                     /gene="yedE"
                     /locus_tag="ACHJ48_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO4708632.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVAAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            19364..19597
                     /gene="yedF"
                     /locus_tag="ACHJ48_003132"
     CDS             19364..19597
                     /gene="yedF"
                     /locus_tag="ACHJ48_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO4708633.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            19706..20374
                     /gene="yedK"
                     /locus_tag="ACHJ48_003133"
     CDS             19706..20374
                     /gene="yedK"
                     /locus_tag="ACHJ48_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO4708634.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEISGKEASEIAASGCVPANQFSWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            20485..20964
                     /gene="yedL"
                     /locus_tag="ACHJ48_003134"
     CDS             20485..20964
                     /gene="yedL"
                     /locus_tag="ACHJ48_003134"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4708635.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
BASE COUNT         5263 a         5371 c         5042 g         5317 t
ORIGIN      
        1 tcgcagcgtt ttttgacgtg gttttgttcg ccttaaccat cctgatattt gctctggaga
       61 ccatttcatc tccagctttt ccagaacaag ctttcgcaat ggtaaatttt gatccagtaa
      121 gcacggtttt ggccttttcg ccattctgtt ggctcggtta ttagcatcaa cagctttgta
      181 atagcgtctg ccccgattac gctgaacttc acgtgagatc gtcgaaggac tgcgattcag
      241 cgcagtagct atcgcacgaa tgctcatttt ggctgacaaa ccagctcgta tctcctcgcg
      301 ctcagacagt gtcaggtgag ctacagcccg cttacgctca tggggtttta tgccgccagt
      361 atcccttaac atagtgaaga tcgttccggg ttttgaaccc aggatattcg ctatttcact
      421 gaagcctgtt ccgttcttcc atagttccaa acctccgcaa ccccatgttt tcacataact
      481 gttgcgttga ccaattgaat ctacacttac attgttgatg aaatcgctca cgaaggacca
      541 ccggacaggc tttcagccag aagcgcagat tttcgggatg tgaaccgtta acggcacata
      601 atgctcgcac aacaaacctg ttaaaagaga ttactcagca ttctcctgct tccattgccg
      661 gatcatttca tcggtaacat caccctcata acataccacg tcataccctc cattacggct
      721 atatgcactg cgtccaccac atctactacc attccttgca tgattataag gacacgcaca
      781 attgcctgga taagattcaa tggattcttt aattatttct tttttgatct gagcatcaga
      841 ctttcctgtt gctgcatatc cactaaaaga tactaaacaa aggcagattg ccattaataa
      901 accacatcgc ataccgatac actcaattac taaccaatca tagcccacat cataaaatcg
      961 agatgaggag cacaatacct ttaacaagaa ggtgtcataa cgataatatg tcgtgcaatt
     1021 catcaaaaaa agatctatat caaagagata gaactcttat cagtgcttca gacatcggca
     1081 ttcgaggcag cagaaaaaca gtaaatctga cgaatttgcc ttttcccaag atgttgtcag
     1141 attaaaaaga tagattagcg caggtacctg ttggtggatt gcaggaggaa aaataaagtc
     1201 gagtggaaaa atcccagaaa aatttttgtt taacagaggt cctggttcac tatgcgaaat
     1261 acggaggagc tgcagaagaa ttctgcttca tccttagagt ttaaacagca cgtgcactac
     1321 cattgtttct gcatatcgcc acaccatcgc cactctaccg ccattgcaca aaatacaaat
     1381 acgaaaaaac cacccgaagg tggtttcacg acactgctta ttgctttgat tttattctta
     1441 gctttcccat ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc cgagggattt
     1501 taaatccctt gtgtctaccg attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt
     1561 ccggagtcga accggactag acggatttgc aatccgctac ataaccgctt tgttaacgcg
     1621 ccaaattctt caggcctttc agccagacat ccgctgacgc cgatgtcttt taaactggag
     1681 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
     1741 gctattcccg cattcatcaa gcaatcagtt aatcacttga ttttattatc gtctggcaat
     1801 cagtgccgcc gttcgatgcg ttgcattcta cttacctggc gcgatgagtc aacgatattt
     1861 ttcaccactt ttgatcgttt gctgaaaatt gcgccgaaac gatcactgat caagcaaatc
     1921 tgcacgcgca gcgctcaaat attgcaacat tgaccacaga gtcagtaccg cagccacaaa
     1981 gaaaagtgca ataccggcgt actcaaccca aatgttcgga cgccacagca gccatgccaa
     2041 cgccaccatc tgggcagtgg ttttcacttt cccaatccag gagacagcca cgctactgcg
     2101 tttacccaac tccgccatcc attcgcgtag cgcagaaata ataatttcac gggcgatcat
     2161 cgttgccgcc ggtaaggtca cccaccagct gtggtaatgc tcggttacca gcaccatggc
     2221 gatagccacg agaactttat ctgctacagg gtcaaggaaa gcaccaaacc gggtactctg
     2281 gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat
     2341 gagcgcggcg gcaaacggcg accaagtgac aggcagataa aagaccaata caaagaatgg
     2401 gataaggatg acacggaaca gtgtaagcaa cgtagggata ttaaattgca taatgacggg
     2461 taactatctg ttgtcagtaa gattacccct atgttgctac agagacatca atgtttcaac
     2521 gaccagaaga tcttttctgc cagaccttgc gaaatacccg gcacttttgc aatttcctcg
     2581 acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat ttgccggcgt
     2641 tttggcccga cgccttcaat ggtttccagg gaactggtat ttttgacctt cgcccgtttt
     2701 ttacggtgcc cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg gataacatgc
     2761 agcgcgggag aatctggcgg caaactgaat ccctcacctt ccggctcaaa gaacagcgtt
     2821 tccagcccag ccttacgatc tgctcctttg gcaacaccaa gtagcagcgg atgattttta
     2881 tcccatgaga catccagttc ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg
     2941 ccgtcgataa gtatcacatc cgggatctta ctgtcgtcga tggctttacc ataacgccga
     3001 cgcagcacct gattcatcgc cgcataatca tcgcccggcg tgatgccagt aatgttatag
     3061 cgccgatact ccgcacgcag cgggccgtta gcatcaaaca ccacacagga agcgacggtt
     3121 tgttcaccca tggtatggct gatgtcaaag cactccatcc gcttcacttc cggcaatttc
     3181 aacacgctgg caagtgctgt aagccgctgg tgaacggtag attgctgcga aagtttgctg
     3241 gttaaggccg tcgccgcatt ggtgcgcgcg agtttcaggt aacgcgccct atcgccacga
     3301 ggtttggttt gaacattaat cttgcgtccc gccagttctg aaagggaatc ggcgagcagc
     3361 gttttatcgc taagattaaa atcgagcagg atctcacccg gtaaggtgcg catctggctg
     3421 ccttgtaaat agaactggcc tacgaaggtt tctaccacct cgctcagttc cgtaccgcca
     3481 ggcactttcg ggaaatagct gcggctgccg agcactttgc cctgacgaat gaacaatacg
     3541 tggacacaag ccatacccgc atcgaacgcc acaccaataa cgtcgaggtc gtcgccggta
     3601 ttggaaacga attgtttttc ggtgacgcgt cgcaccgcct gaatttggtc gcgaatacgt
     3661 gccgcttctt caaactccag attctgactg gcagtttcca tacggctaat gagttgcgta
     3721 agcacctgat catctttgcc agacaaaaac aggcgcacat actcgacctg ctgagcgtat
     3781 tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac agcgccctat ctgatattgc
     3841 agacacggac gcgagcgatt gcgataaaca ctattttcgc actggcgaat ggggaaaatc
     3901 ttttgcagta gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg gccgaaatat
     3961 tcacctttgg catgctttgc accacgatgc atcgccagac gcgggtgggt atcgccactc
     4021 aggaagataa aaggatatga tttatcatcg cgtagcaaaa cgttgtaacg cggctgatag
     4081 agtttgatgt agttgtgttc cagcaacagc gcttcggttt ctgtatgagt aaccgttaca
     4141 tcaatttgct ggatctgggc gaccagcgct tcggttttgc gcgaagcgag gttgctacgg
     4201 aaatagctgg aaagccgttt tttcaggtct ttcgctttgc cgacatagat aaccgtacca
     4261 ccagcatcgt acatgcgata aacgccaggc tggctggtta cggtttttaa aaacgctttt
     4321 gcgtcaaact gatcactcac tgacttgata atgtctccgc attacacaga ccatggcgaa
     4381 ttgccaggtg agtcagctca acatcgccat gaatgtttag tttactgaac atacgatagc
     4441 ggtagctgtt caccgttttc ggactgagat tgagctgttc tgagatctca ttgaccttct
     4501 ggcccttcgt gatcatcagc ataatctgca attcacgttc agacaaactg gcaaatgggc
     4561 tttctgtttt ttctggttcg atctggctta atgccatttg ttgagcgatg tcagaagcaa
     4621 tgtaacgctg cccagaatag acagaacgga tcgcactcac gacttcctgc ggagccgcgc
     4681 ctttgctgag gtagcccgca gcaccggcct gcatgacttt cgctggtaaa gggttttctg
     4741 tatggacggt aagcatgatg atttttacat cagctgtgga acgcgcgatt ttacgcgtcg
     4801 cctcaagacc gccaatgccc ggcatactca tgtccattag caccacgtca acggcatttg
     4861 cccggcacca cttaacggcg tcttcaccgc acgatgcctc accgacgact tttataccct
     4921 ttatatcttc cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta
     4981 gaacgttgat caaaggaata tctccagaaa tagggataac gctactgata gttagtcatt
     5041 cgtatattag cggtttttat tgcaactttg aaacgttaaa aatggtgggt attcgatttt
     5101 tctctcgttt ttgtcaattc gattgtccac agttatggtc acgcccgtca ccaacggctt
     5161 accggatcgt ttcacagtaa gccctgcgac gaaatgtgac aaaaattacc tttattcagc
     5221 aaaaatgaaa atcagcccgc tgaatatgta acattaatta acccgaacaa tacgggcaaa
     5281 aaacattcat gataaaaata tttattggtc attatattaa cgcatttaat agcaccgttg
     5341 acggcacgct caaaaaacaa ccactgctat ttttagcaaa gcttatggta tactccgccg
     5401 ccttaacatt tttcaccgca aattttcatt gcaacatgac gaggaaaata aatgagtacg
     5461 cctgattttt ctactgccga gaataatcaa gaactggcaa atgaagtctc ctgcctgaaa
     5521 gcgatgctga cgctgatgct gcaggcgatg ggacaagctg acgcgggccg cgtgatgtta
     5581 aagatggaac aacagcttgc gctgatcgaa gacgaaaccc aggctgcagt attttccaaa
     5641 acggttaagc aaattaaaca agcctaccgt cagtaatata aaaccggctg atagcgtgcc
     5701 tttcagccgg tttttgcatc tggcacgcag gacagaaaag agatcaaatt aagccagtag
     5761 cggccgcgta acaggcaacc tgggtcttat ttggtgcatt aatttttttc tgcatgtttt
     5821 tctggtggaa attgaccgta ttctcagaga ttgacaaaat catcgctatc tctgctgatg
     5881 tcttcccttc cgccgtccac ctcagaattt ctttttcgcg cttgctgaaa ttcatctctg
     5941 gcgtcatcac tatttcatca tttaaacgca tcagagccat cagactttcg cgcaccagta
     6001 actgcatttt taattgcagt tcatcactaa gaatgggtat ttcgcgcgcg ctgcaacggg
     6061 aaaaggacaa aaagcccagc gcccggtttg gcagcattaa atactgagtg acaccgcggc
     6121 gtaaaccatg tgcgcgcgcg gcttcccata acggctgtgc ttcgttgaat aagtcatcat
     6181 tccacattag atggccctga ctaaagtttt cagggttcag caccggatca attgcgagaa
     6241 agttttttgc ctgataataa ctaacccacg cctcagggta attggtgtag aaagccactt
     6301 taggtcgagt gaatggtacc gggtggcgga cacataacga atagtaatcg tactccagct
     6361 gctgagcctg aagctcaatt tcatggtaga cctcttctgc ggtctccatc ctctgaaaac
     6421 gcaacagcat cgtgcgacgc cagctgaaaa aatccgtatc ctgcatagta aaccgcaacg
     6481 cccctgagag tgagtatcat ttataatgat aattcaaact atcacataaa taagatttat
     6541 atataattta tattattcag gcaatgaatt acttttgcaa gccatcgcat tctcttatgt
     6601 tattaatgag ttatgctgat tttttaagca gttttatcag gcttgaaatg gcgttccgcc
     6661 ccgactgtcg aatcgtcggg gctgattttt tcttattatt gcagcagaaa cttctcgagg
     6721 aactggcggg tgcgaggctg ctcgggatcg gcaaataacg cttttgcggc cccctgctcg
     6781 actatccgcc cctggtccat aaaaatcgcc cggtccgcaa catcccgggc aaagctcatt
     6841 tcgtgtgtca caatcaccat cgtgcgcttt tcctgcgcca gctgacggat ggtgttcagg
     6901 acttcgccca ccagctctgg atccagcgct gacgttggct cgtcaaacaa aatcacctca
     6961 ggacgcattg ccagcgcacg cgcaatcgca acacgctgct gttgaccgcc agacaaacga
     7021 cgtggatagc tggtttcttt accggccagc ccaactttcg ccaacagctt gcgagcgcgc
     7081 gccgtggcct cttctttcgg ttcgcctttg acgatcaccg gcccttcaat aatgttctcc
     7141 agcaccgtac gatgcggaaa caaattaaag ttctggaaga caaacccgac gtgctgacgc
     7201 aactggcgaa tcagcgattt ttgctgactt aatgaacgtg cagtatcaat agtgatatcg
     7261 ccaacggtga tcgttcccgc ttccggttgt tccagcagat ttatgctacg tagcaatgtg
     7321 gttttgccgg aaccactcgg accgataatt gccaccactt cgccaggctt tacctcgagg
     7381 tcgataccgt gcagcaccgt ctgaccgtgg aattttttca ctaggttctt aacttcaatg
     7441 gcactcattt gggttctctc tcctggcgat taagttgatt ctcaaaatgg ttctgcaacg
     7501 tcgataacac agtcgccatg atccagtaga tgagcgaagc cgccagatac atggtgaata
     7561 cctccagcgt acgcgaggta atcaactgcg cctgacggaa cagctccggc acctggattg
     7621 tcgcggccag cgacgtatct tttaccagac tgataaagct gttcgagagc ggcggcaacg
     7681 ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatagt ctgccatggc gtcataccaa
     7741 tactggcagc tgcttcccac tgacctttat caatagaaga aatggcggcg cgaagcgttt
     7801 cggcagtata ggcggcagtg ttgagcgaca gaccaatcat tgctgacgga atgggatcta
     7861 attcgatacc aaactgcggc aggccgtaat agatcataaa cagttgtgcg atgagcggcg
     7921 taccacgaaa aatagagata taaaaacgcg ccagccagcg cacgggccag attggcgaca
     7981 ggcgcatcag cgcgagaata aaccctagca gtaaaccaaa aaacatgccg ccgatactga
     8041 gttgcagcgt atacccagcc cctttcagca ggaacggcaa ggaatcaata accagttgta
     8101 tactttcttg catgagcgtt cccggagcta aacgtgggga tgataggcga acagcgcagg
     8161 cgcgccgccg gtatgaataa acagaatcgg cccttcatct ttgaagcgtt tctgactgat
     8221 accgtcaatc agacccgcca tcgcttttcc ggtatacaca ggatcaagca gaatgccttc
     8281 cagccgcgcc agcaatttca ctgcttccat gccttcgtcg ttcggcacgc cgtagccagg
     8341 tgcaaaatag tcatcccaga gtaaaatttc cactgatgcg gtcagctcca gttctttcgc
     8401 aatcgcctgt tgtaggttaa ccactttcgg caattgatcg gcaacggaac gcgacacggt
     8461 cacgccaatc agttcgcttt caggcatcag gtgttccagc ccaacagcca gtccggcgtg
     8521 agttccggca ctgcccgatg cgactaccac cgatgaaata ttaaccgccc cttcacactg
     8581 ttgcgcgatt tccagcgcac tctccacata acctagcgcg ccaagagcat tagaaccgcc
     8641 aaccggaatg acatacgggc gaaagccttg tgcttcgact cgcgtcgcca gctcttccag
     8701 ttgggcattg ggatcggtca gtgcgtcgca catttcaatc tgggtattga acagatccag
     8761 caacaagcga ttgccgttgg ttaaatagtt ttctgcggtt gtgccaatag gattttccag
     8821 cagcgccacg cagtgcagac cgagtttggc agcgactgcg gcagtctgcc gcacatggtt
     8881 agactggatc gccccggcag taatcagcgt atcggcacct tcacgcagag catctgccgc
     8941 gagaaattct agcttacgta atttattgcc gcccattgcc atgggggtga cgtcatcccg
     9001 tttgatgaaa atttcccgtc ctagataatc agaaaagcgc ggcagatatt cgagcggcgt
     9061 tggcgcgccg ataaactcca gccgtggaaa acgggttaaa ttatgcagtg gcataacagc
     9121 ctccgatgtg tgttgttgtg attttctcat tatgcacgct gaaaacgcgt aaataaaaaa
     9181 ggcgctagtg aaagcgccct tttttgtcat tatgctgatt atttggtcac atcagcacca
     9241 aaccattttt cggaaagggc ttgcaaagtg ccgtcttttt gcatttccgc aattgcatca
     9301 ttcactgctt tcagcaggtc ttcatttcct ttacgcagcg ccacgccaga ctcctgacgg
     9361 gagaatgctt caccggttac tgccagcgta tcgttggttt tcttcaccag atccagcgcc
     9421 gccagacgat caacgaggat cgcatcgata cgccctacgc gcagatcctg atatttggtc
     9481 gggtcatcat cataggtacg cacatcgacg ccctgaacat tctgccgcag ccactcttca
     9541 tagttggtgc ccagaccgac ccccactttt ttgcctttca gatcatcggc tgttttaatg
     9601 gtgccttcgt tacctttttt caccagcgcc tgaataccag aaatggtgta cggggttgag
     9661 aaatcgtatt ttttcttgcg ctcatcagaa atggtgacct gattaatcac cacatcaata
     9721 cgtttagagt ccagcgacgc cagcataccg tcccatttgg tcggttttag tgacgcctca
     9781 acgccaagat gttttgccag ctgttgggca aattccactt caaaaccggt taatttgccg
     9841 tcatccccct gaaaactgaa cggcggataa gtaccttcca gccctaccag cagcgtgccg
     9901 cgctctttaa ctttattaag cagaccttca tctgcaaaac ttttaacgct catgcccgca
     9961 atcagcgcta cggccatcac acccatcaat gcctgacgtc ccagatgtgc taatttcata
    10021 ttcaccccga atgttgttat gtctgtttgc agtgtagagc cattgtttgt aaacacaaaa
    10081 acaactccgc tacatcttat tcttatttaa tatatatcag aagcaggcag gctggaggat
    10141 ttctgcacaa gtccggtaca ggtttggcgc tgatagtgtt gatacttccg taacgcgatg
    10201 cggtaattgt tggtgctggt ggtgggcagc cacggggcga ggatttcatc aaggaaaccg
    10261 tcctgcagct gatcgaggga aatattttgc tcgtgtagat gattccccaa acggcgcaga
    10321 cgaacgacat attcacgcac cgtaccaggg ctcatttcag tttgctcaaa aagaaattgc
    10381 ttaaagccga taatatcgaa aaaatcactc tgcgttttgc aatgtaaatc accgcaaaat
    10441 cggcacaatg ccgcccatga tttttgttcc gtttgccagc cattttcatc aagcagcgtg
    10501 tccaggcggg aaatctcgat tttattcact attttgccgt cgcgaactaa ggtaatgcga
    10561 tcgagcaatt tacggcaatg cgcgcaatgg gtctggctgt gtttaaagtc tttaagatag
    10621 cggcttaatg gccgtctttt caggtgctgc accatcatta agaactcctg gtagtcaaag
    10681 ttaaagtgcg gcatttactg acgttataac ttacccagtt tagtgcgtaa ccgtttaata
    10741 gcctggctgt gtaactgact gacccgcgat tccccgacct ccagcactgc accaatctct
    10801 ttgagattca gctcttcctg gtaatagagg gttaatacca gtttttcgcg ctccggcaac
    10861 gtttcgatag cttccatcac ccgctggcgc agattactgt ccagtagttg ttgtagcggg
    10921 ttttctcgtt gatgatcatc agtaaccaat tcgatgctat cgccgtgctc ttcgcgccac
    10981 tcatcgtagg agaagagctg gctgttattg gtgtcgagca acatttggcg ataatcggca
    11041 atatcgatcc ctaaacgttc cgctacctca gtttccgtgg cgttgcggcc aagttcctgc
    11101 tccagttgcc ctattgcctg tgccacttca cgcgcgttgc gtcgcacgct gcgcggcacc
    11161 cagtcacggc tgcgaagttc atccagcata gcgccacgga tacgctgcac tgcgtaagtt
    11221 gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg cattaagtaa cccaatgccg
    11281 cccgcctgta gcagatcgtc aagttccacg ctcgcgggca gtcgaacctg caggcgcaat
    11341 gcttcgtgac gcaccagcgg gacataacgc tgccacagcg agtgtttatc cattacacct
    11401 tcagcggtat agagtgaatt cacgataaac agccctgcgt tatatgagtt atcggcatga
    11461 ttatccgttt ctgcagggtt tttaatcgga cgattagtgg gtgaaatgag gggttatttg
    11521 ggggttacag gtaaattcca ggcagaaaaa accccgccgg tggcggggaa gcacgttgct
    11581 gacaaattgc gctttatgtt gccggatgcg gcgtaaacgc cttatccggc ctacaagaat
    11641 gtgcaaattc aataaattgc aattcaactt gtaggcctga taagcgcagc gcatcaggca
    11701 atttggcgtt gccgtcagtc tcagttaatc aggttacaac gattaaccct gcagcagaga
    11761 cagaacctgc tgcggtacct ggttagcttt tgccagcacg gagttaccgg cctgctgaat
    11821 gatctgcgct ttcgacatgt tggacacttc ggtcgcatag tcggcgtcct gaatacggga
    11881 ctgcgcttcg gacaggttgg tagtggtgtt gttcaggttg gtgactgcgg aatccagacg
    11941 gttctggata gcacccaggg aagaacggaa tttgtcgatg gagctgattg cgtcgtccag
    12001 ggcagcaagc gggttggtcg ttgcagaacc agcactggta gtctcagtag tgattttacc
    12061 cgcggagttc acatttacag cagtaccaat tgctggagca taatctttat tggtatcagt
    12121 cgctgaagca tacccggcaa cagtcacaga gccgttatcc ttgttaacgc tgtaagagac
    12181 tgtatagtcg gcaacgttag tgataccacc tttatcatcc acataagact tcgcagcatc
    12241 actggattca cccgcggtaa acccaatagt tttgctcagt acaccggaat taaaggtaat
    12301 tgaagatgtc gcggcagccg ctttggtttc gctcaatact acatctttac tcactgtagc
    12361 ctgataagta atcccaccag ggattaacgg agctactgga gtggttgtcg caggagttgc
    12421 ttttgcgatg gtatattctg tgccattgaa tgtcagagag gcaccgtcac taccttcgct
    12481 cgctgctttg agcagcgctt tcatatcagc tttagctgcg ctaccagcgt tgttagtcgt
    12541 caagttgcct gcatcgtcta cgtatgcctg gcttccaccg atggtgatat tacctgctga
    12601 atccgtttcg aaagaaacag taccatcttt tgtggtgtaa gaaccattaa cagatttacc
    12661 cacatcagca tgaagggtgg cagctaacgc atcacgtttt gaagctgaat cagcagcctg
    12721 cgccgcggcg tctacacccg taccagcagt agatttagtg gtagtaaaat caccagattt
    12781 agcgttgtaa gtataatcta cgccgccaac tgtgacttta tcgccattcc ctaatttatc
    12841 gaatgcagca tcggtagtta acaaggtatt ttcggttttc agatcataaa gacctgtcgt
    12901 ggtgtttaac ttcgcgccag cagaagttaa atcacttacc gttgcagctt tgttggtaat
    12961 agtaccttta ccatttacgt taaagccatt cagacccaga gtatcagaat cgattttttt
    13021 caggtcgatc gtgatggttt cgccgtcatt cgcaccaacc tgaattttca ttgaaccgtc
    13081 tttcgccagc acgttcacgc cgttgaactg ggtctggccg gatacgcggt caatttcatc
    13141 aagacgagat ttgatttcgt cctggatgga gtccaggtca gaatcggagt tagtccctgt
    13201 agtggcctga accgtcagtt cacgaatacg ctgtaagttg ttgttgattt cggacagcgc
    13261 gccttcggtg gtctgcgcaa cagaaatacc gtcgttggcg ttacgggccg cctgagtcag
    13321 gcctttaatg ttagaagtaa aacggttagc aatcgcctga cctgcggcgt catccttcgc
    13381 gctgttaata cgcaagccag aagacagacg ctcgatagaa ctcgacagcg cagactggtt
    13441 cttgttgata ttattttgag tgatcagcga gaggctgttg gtattaatga cttgtgccat
    13501 gattcgttat cctatattgc aagtcgttga ttacgtattg ggtttccacc cgtcggctca
    13561 atcgccgtca aacctgttat cgtctgtcgt aaaacaacct ttagaatttt tttcacaaac
    13621 agccattttt tgttagtcgc cgaaatactc ttttctctgc cccttattcc cgctattaaa
    13681 aaaaacaatt aaacgtaaac tttgcgcaat tcaggccgat aaccccggta ttcgttttac
    13741 gtgtcgaatg ataaaaggaa atcgcatggc aagtatttca tcgctgggag tcgggtcagg
    13801 tctggattta agttcaatcc ttgatagcct caccgccgcg caaaaagcga cgctaacccc
    13861 catttcaaat cagcaatcgt cgtttaccgc taaacttagc gcctacggta cgctgaaaag
    13921 cgcgctgacg actttccaga ccgccaatac tgcattgtct aaagccgatc ttttttccgc
    13981 caccagcagc accaccgcgt tcagtgccac caccgcgggt aacgccatcg ccgggaaata
    14041 caccatcagc gtcacccatc tggcgcaggc gcaaaccctg accacgcgca ccaccagaga
    14101 cgatacgaaa acggcgatcg ccaccagcga cagtaaactc accattcaac aaggcggcga
    14161 caaagatccg attaccattg atatcagcgc ggctaactcg tcgttaagcg ggatccgtga
    14221 tgccatcaac aacgcaaaag caggtgttag cgcgagcatt attaacgtgg gtaacggtga
    14281 ttatcgtctg tcagtcacat caaatgacac cggccttgac aatgcgatga cgctctcggt
    14341 tagcggtgat gatgcgctac aaagttttat gggctatgac gccagtgcca gcagcaacgg
    14401 catggaggtc tcggtcgccg cccagaatgc gcagctgacg gtcaacaacg tcgccattga
    14461 gaacagcagc aacaccatca gcgacgcgct ggaaaacatc accctgaacc tgaacgatgt
    14521 caccacgggc aaccagacgc taaccatcac tcaggacacc tccaaagcgc aaacggcgat
    14581 taaagattgg gtgaatgcct ataactcgct aatagatacc ttcagcagcc tgaccaaata
    14641 caccgccgta gatgcgggag ctgatagcca gagttctagc aatggcgcac tgctcggcga
    14701 ctccacgctg cggacgattc agacgcagtt gaagtcgatg ctgagtaata ccgtcagttc
    14761 ttccagctat aaaacgttgg cgcagattgg tatcacgacc gatcccagcg atggcaaact
    14821 ggaactggat gccgacaaac tcaccgctgc actgaaaaaa gatgccagcg gcgtaggtgc
    14881 attgattgtt ggcgatggta aaaaaaccgg catcacgacc accatcggca gcaacctgac
    14941 cagttggctt tcgacaacgg gcattattaa agccgctacc gatggcgtta gtaagaccct
    15001 gaataaatta actaaagact acaacgccgc cagcgatcgc attgacgcgc aggtcgcgcg
    15061 ctacaaagaa caatttaccc aactggacgt tttaatgacc tcgttaaaca gcacaagcag
    15121 ctacttaacg cagcagttcg aaaacaacag taattccaag taatcattat tcatcgggag
    15181 acaggtcatg tacgcggcaa aaggcaccca agcctatgca caaattggcg tcgaaagcgc
    15241 cgtaatgagc gccagccagc agcagctggt caccatgcta tttgatggag tgctgagcgc
    15301 actggttaga gcgcgcctgt ttatgcagga caacaatcag caaggcaaag gcgtctcttt
    15361 gtcaaaagcg atcaacatca ttgagaacgg actgcgggtg agtcttgatg aagagagcaa
    15421 agacgaacta acccaaaact tgattgctct ttatagctat atggtcaggc gtttgctgca
    15481 agccaattta cgcaacgatg tctccgcagt cgaagaagtg gaaacattaa tgcgcaatat
    15541 tgccgatgcc tggaaagagt cgttactctc cccttctttg attcaggacc cagtctgatg
    15601 aaccatgcac cgcatttata tttcgcctgg caacaactcg tcgaaaaaag ccagctcatg
    15661 ttacgcctgg caacggaaga acaatgggac gaactcatcg ccagcgaaat ggcgtatgtt
    15721 aatgcggtgc aggagattgc acatttgact gaagaggttg acccgtccac cacgatgcag
    15781 gagcagctcc gcccgatgct gcgcctgatt ctcgacaacg aaagcaaggt aaagcagtta
    15841 ttacagattc ggatggatga actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg
    15901 gtgttgagtg cctatggcga tcagggcggc tttgtgctgg ctccgcagga taacctcttt
    15961 tgaatctgaa tgagtcgatg gctcgcgaat aatccgatta cggctacgct tctaatgttc
    16021 cccttgaatg gagtcgaaga atgcgtaatc ccacgctgtt acaatgtttt cactggtatt
    16081 acccggaagg cggtaagctc tggcctgaat tggccgagcg cgccgacggt tttaatgata
    16141 ttggtatcaa tatggtctgg ttgccgcccg cctataaagg cgcatcgggc gggtattcgg
    16201 tcggctacga ctcctatgat ttatttgatt taggtgagtt tgatcagaaa ggcagcatcc
    16261 ctactaaata tggcgataaa gtacaactgc tggccgccat tgacgccctg aaacgtaatg
    16321 acattgcggt gctgttggac gtggtagtca accacaaaat gggcgcggat gaaaaagaag
    16381 ctattcgcgt gcagcgcgtt aatgctgatg accgtacgca aattgatgaa gaaatcattg
    16441 aatgcgaagg ctggacgcgt tacaccttcc ccgctcgtgc cgggcaatac tcacagttta
    16501 tctgggattt caaatgtttt agcggtatcg accatatcga aaaccccaat gaagatggca
    16561 tttttaaaat tgttaacgac tacaccggcg aaggctggaa cgatcaggtt gatgatgaac
    16621 tcggtaattt cgattatctg atgggcgaga atatcgattt tcgcaatcat gccgtgacgg
    16681 aagagattaa atactgggcg cgctgggtga tggaacaaac gcaatgcgac ggttttcgtc
    16741 ttgatgcggt caaacatatt ccagcctggt tttataaaga gtggatcgaa cacgtacagg
    16801 aagttgcgcc aaagccgctg tttattgtgg cggagtactg gtcgcatgaa gttgataagc
    16861 tgcaaacgta tattgatcag gtggaaggca aaaccatgct gtttgatgcg ccgctgcaga
    16921 tgaaattcca tgaagcatcg cgcatgggac gcaactacga catgacgcag attttcactg
    16981 ggacgctggt ggaagccgat ccttttcacg ccgtaaccct cgttgccaac cacgacaccc
    17041 aaccgttgca agccctcgaa gcaccggtcg aaccgtggtt taaaccgctg gcgtatgctc
    17101 taattttgtt gcgggaaaat ggcgttcctt cggtgttcta tccggacctc tacggtgcgc
    17161 attacgaaga tgtcggtggt gacgggcaaa cctatccgat agatatgcca ataatcgaac
    17221 agcttgatga gttaattctc gcccgtcagc gtttcgccca cggtgtacag acgttatttt
    17281 tcgaccatcc gaactgcatt gcctttagcc gcagtggcac cgacgaatat cccggctgcg
    17341 tggtggtcat gtcgaacgga gatgatggcg aaaaaaccat tcatttagga gagaattacg
    17401 gcaataaaac ctggcgtgat tttcttggga accggcaaga gagtgtagta accgacgaaa
    17461 acggcgaagc gaccttcttt tgcaacggcg gcagcgttag cgtgtgggtt atcgaagagg
    17521 tgctttaaat tcatccccgg cggcaagccg gggagatttc attacggcaa tggcgtcggt
    17581 aacggcgctt tatccagcgc agcggcacac tctgccgtcg ggcgatccac acgctccatc
    17641 gtcataccat cgtactcaat ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt
    17701 acggtaacgt tagtgagatc atcagacatc aatgtcaact tgcccggcac agcaatcacc
    17761 cgctgccact gacggcaatc tagggtatcg ccctctttag tgacaatcag gctgcctatc
    17821 gcttccgggc tgaccagcgc acgttgcggc cctttggtct gccagtagcc cgccagccag
    17881 tccggcgcgg gggttttcac gacattgtta tagttttcga cttcggcaca gcccgccagc
    17941 aacatgagcg cgcctgcaat tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag
    18001 aggattgtgg cattaaagcc ctgatgtcgc cagcctttcc aggttcagag agaaatttga
    18061 tctaactatt atttttgtga attattaccc cgaaaataaa tttgcatggt gtatgattcg
    18121 cgactcttgt ttcaaacctt ctgagttcag aggctacatt catgtcatgg cagcaattca
    18181 aacacgcctg gttgattaaa ttctgggcgc ccatccccgc ggtcatcgcg gcgggtattc
    18241 tctccactta ctattttggc atcactggca ccttttgggc tgtcacgggt gaatttaccc
    18301 gttggggcgg tcagctcctg cagctgttcg gcgtccatgc tgaagagtgg ggttatttta
    18361 aaattatcca tctggaagga tcgccattaa cccgcatcga cgggatgatg atcctcggta
    18421 tgtttggcgg ctgctttgcc gcagcgctgt gggccaacaa tgtcaaacta cgcatgccgc
    18481 gtagccgtat ccgcattatg caggccatta ttggcggcat tatcgccggt tttggcgcgc
    18541 gtctggcaat gggctgtaac ctggcggcat tctttaccgg tattcctcag ttctcgctgc
    18601 atgcctggtt ctttgccatc gccactgcca ttggttcatg gtttggcgcg cgctttaccc
    18661 tgctgcccat cttccgtatt cccgtgaaaa tgcagaaagt tgctgccgcc tcaccgctga
    18721 cgcaaaaacc ggatcaggcg cggcgtcgtt ttcgtctcgg gatgctggtc tttttcggca
    18781 tgctgggctg ggcgctgctc acagcgatga accaacccaa actggggctg gcaatgctgt
    18841 ttggcgtcgg ctttggttta ctgattgaac gtgcgcaaat ctgctttact tcggcgttcc
    18901 gcgatatgtg gatcaccgga cgtacccata tggcgaaagc aatcattatt ggtatggcgg
    18961 tgagtgccat cgggatcttc agttacgtac agttaggcgt tgaacccaaa atcatgtggg
    19021 cgggaccaaa cgcggtaatt ggtggtttac tgtttggttt tggcatcgtg ctggctggcg
    19081 gctgcgaaac cggctggatg taccgcgcgg tagaaggcca ggtgcactac tggtgggtcg
    19141 gtctgggcaa tgtgatcggc tcaacgattc tggcgtatta ctgggatgat ttcgctccgg
    19201 cgctggccac cgactgggac aaaatcaact tgctgaaaac ctttggtccg atggggggcc
    19261 tgctggtgac atatttgctg ttgtttgctg cgctgatgtt gattatcggc tgggaaaaac
    19321 gcttcttccg ccgtgcggca ccgcagactg ctaaggagat cgcatgaaaa atatcgttcc
    19381 tgattaccgc cttgatatgg tgggtgaacc ctgcccttat ccggcagtcg caacccttga
    19441 ggcgatgccg cagttaaaaa aaggggaaat cctggaagtg gtgagcgact gtccgcagtc
    19501 gatcaataat attccactgg atgcgcgtaa tcacggctat acggtgctgg atattcagca
    19561 agacgggccg accattcgtt atttaattca gaagtaatcc ctcattcccg ctgtgaccca
    19621 cagcgggaac atcgttacct ttgtatcaca tagattttac ccgccccctc tcctattctt
    19681 ttcagtatga tctcaggagg tttctatgtg tggacgcttt gcccaatccc aaacgcgtga
    19741 agattacctt gcgcttctcg cggaagatat tgaacgcgat attccctatg atcccgaacc
    19801 cattggcaga tacaacgtcg cgccgggaac caaagtccta ctgctcagtg aacgtgatga
    19861 acaccttcat ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc
    19921 gctgattaac gcccgcgtag aaactgcggc aaccagtcgt atgtttaaac cgctctggca
    19981 acatggtcgg gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa
    20041 aaagcagcct tattttatct atcgtgctga tggacaacct gtttttatgg cagcgatagg
    20101 cagcacaccg ttcgagcgcg gtgacgaagc cgaaggattt ttgattgtca ctgcagcagc
    20161 agaccagggt ctggtagata ttcatgaccg ccgcccgctg gttctgtcgc cagaagctgc
    20221 gcgggaatgg atgcggcaag agattagcgg taaagaagcc tcagaaatcg cggccagtgg
    20281 ctgtgttcca gcaaaccagt tctcctggca cccggtatcg cgcgcggtgg gtaatgttaa
    20341 aaaccagggt gcggagttaa ttcaacctgt ttgataagcc tggagattat tgatcaacaa
    20401 tactgcgtca taagtaatct ctattagaca aagatttcat tacctgttgg catattgcaa
    20461 aaataacacc aatacggaat cgtcatgttc acgattaaaa cagatgatct cacccatcca
    20521 gcagtgcaag cattagtggc ttaccatatt tccggcatgc tgcagcagtc tccccctgaa
    20581 agcagtcatg ctttagacgt gcaaaaatta cgtaacccga cagtgacatt ctggtcagta
    20641 tgggaaggcg aacaactcgc aggaattggt gcgctgaagt tgctggatga taaacatggc
    20701 gaactgaaat caatgcggac cgcgccaaat tatttacgtc ggggtgtcgc cagtctgatt
    20761 ttacgccaca ttttgcaggt cgcccatgac agatgccttc atcgcctgag tttagaaacg
    20821 ggtacacagg ctggatttac ggcctgccat caactttatt tgaagcatgg tttcgttgat
    20881 tgcgaaccgt ttgccgatta tcaacttgat ccacacagtc gatttttgtc attgacgcta
    20941 tgcgaagata atgagttgct ttgagccaga cgcagcacat tcttgcattc gac
//
LOCUS       DAZFDC010000083        20266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFDC010000083 DAZFDC010000000
VERSION     DAZFDC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.083"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(120..866)
                     /gene="cutC"
                     /locus_tag="ACHJ48_003135"
     CDS             complement(120..866)
                     /gene="cutC"
                     /locus_tag="ACHJ48_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO4708636.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(880..1446)
                     /gene="yecM"
                     /locus_tag="ACHJ48_003136"
     CDS             complement(880..1446)
                     /gene="yecM"
                     /locus_tag="ACHJ48_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4708637.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQIAHWQFSIVELPWPR
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1662..3395
                     /gene="argS"
                     /locus_tag="ACHJ48_003137"
     CDS             1662..3395
                     /gene="argS"
                     /locus_tag="ACHJ48_003137"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO4708638.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTHDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3437..3481)
                     /locus_tag="ACHJ48_003138"
     CDS             complement(3437..3481)
                     /locus_tag="ACHJ48_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708639.1"
                     /translation="MPIFRIHLDGNKKA"
     gene            3572..4060
                     /gene="yecT"
                     /locus_tag="ACHJ48_003139"
     CDS             3572..4060
                     /gene="yecT"
                     /locus_tag="ACHJ48_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO4708640.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSNPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4180..4572)
                     /gene="flhe"
                     /locus_tag="ACHJ48_003140"
     CDS             complement(4180..4572)
                     /gene="flhe"
                     /locus_tag="ACHJ48_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO4708641.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPSLKVQRNEVIVNYR"
     gene            complement(4572..6650)
                     /gene="flhA"
                     /locus_tag="ACHJ48_003141"
     CDS             complement(4572..6650)
                     /gene="flhA"
                     /locus_tag="ACHJ48_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO4708642.1"
                     /translation="MSNLAAMLRLPTNLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6643..7791)
                     /gene="flhB"
                     /locus_tag="ACHJ48_003142"
     CDS             complement(6643..7791)
                     /gene="flhB"
                     /locus_tag="ACHJ48_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO4708643.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPHMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHVEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7993..8637)
                     /gene="cheZ"
                     /locus_tag="ACHJ48_003143"
     CDS             complement(7993..8637)
                     /gene="cheZ"
                     /locus_tag="ACHJ48_003143"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO4708644.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8648..9037)
                     /gene="cheY"
                     /locus_tag="ACHJ48_003144"
     CDS             complement(8648..9037)
                     /gene="cheY"
                     /locus_tag="ACHJ48_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO4708645.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9052..10101)
                     /gene="cheB"
                     /locus_tag="ACHJ48_003145"
     CDS             complement(9052..10101)
                     /gene="cheB"
                     /locus_tag="ACHJ48_003145"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO4708646.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLNQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10104..10964)
                     /gene="cheR"
                     /locus_tag="ACHJ48_003146"
     CDS             complement(10104..10964)
                     /gene="cheR"
                     /locus_tag="ACHJ48_003146"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO4708647.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFASLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10983..12584)
                     /gene="tap"
                     /locus_tag="ACHJ48_003147"
     CDS             complement(10983..12584)
                     /gene="tap"
                     /locus_tag="ACHJ48_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO4708648.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTTLNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEIIHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12630..14291)
                     /gene="tar"
                     /locus_tag="ACHJ48_003148"
     CDS             complement(12630..14291)
                     /gene="tar"
                     /locus_tag="ACHJ48_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO4708649.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVDAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDHVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14434..14937)
                     /gene="cheW"
                     /locus_tag="ACHJ48_003149"
     CDS             complement(14434..14937)
                     /gene="cheW"
                     /locus_tag="ACHJ48_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO4708650.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14958..16922)
                     /gene="cheA"
                     /locus_tag="ACHJ48_003150"
     CDS             complement(14958..16922)
                     /gene="cheA"
                     /locus_tag="ACHJ48_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO4708651.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16927..17853)
                     /gene="motB"
                     /locus_tag="ACHJ48_003151"
     CDS             complement(16927..17853)
                     /gene="motB"
                     /locus_tag="ACHJ48_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO4708652.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17850..18737)
                     /gene="motA"
                     /locus_tag="ACHJ48_003152"
     CDS             complement(17850..18737)
                     /gene="motA"
                     /locus_tag="ACHJ48_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO4708653.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRIIADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18864..19442)
                     /gene="flhC"
                     /locus_tag="ACHJ48_003153"
     CDS             complement(18864..19442)
                     /gene="flhC"
                     /locus_tag="ACHJ48_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO4708654.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19445..19795)
                     /gene="flhD"
                     /locus_tag="ACHJ48_003154"
     CDS             complement(19445..19795)
                     /gene="flhD"
                     /locus_tag="ACHJ48_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO4708655.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITLLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4872 a         5561 c         4928 g         4905 t
ORIGIN      
        1 agatcgcggt aatcgttcat tttttatact attcagggat aaaaaaaggc ccctgttgaa
       61 attgcagggg cctggtacga gcaagcatca tattgggcga catgatgcaa cggtaaaaat
      121 catttggcct gatggcgttc aatgattcct ttcatttcag caaccgccgc cccgtctacg
      181 acataacgcg aatactcgtc cgcgtgtgca tctgatgaca tggacaatcc ttgattacga
      241 taacgcatcg gtgaggcttg ccacgctccc gcggagctat ggacttccag cactccggca
      301 tcgaggaagt ggtgcaggtt ttctgcacgg actcctgctc cggccataat gattggagca
      361 tcacgatggg caataagttc cataattttt gataaacctt gcagtgcgtc tgatttttgc
      421 cctgatgtca gtacccgggc aatgcccaat tccgcaagat tattgagtgt atttagaggg
      481 ttagcgcaca tatcaaaggc gcgatgaaaa gtcactgcca gcggaccggc agcagccatt
      541 attttttcca ttcgtgacat atcgacatta ccgtcaacat cgagcacgcc cgtcacgagt
      601 ccaggaaaac ctaattcgcg gaccgtgcgc acatcctcaa gaatggcggc aaactcaccg
      661 tcgctgtagc aaaaatcacc accgcgtggg cgaattatcg gatgcacagg gatcgtcacc
      721 cgctggcgca cggatttcag tacacccaac gacggcgtta agcccccctc ttttggggct
      781 gcgcataatt caactctgtc tgcgccgttt tgctgcgccg ttagcgcgca ttccatgctg
      841 taacagcaaa tttccagtaa tgccattttt gctccttaat tacgccgact gctcgctggc
      901 aacaatctct tcaatggacc acggatgaaa tttaatggtc gttttgccat cggtgaccgc
      961 cagcgtcgga ttcggtaatc gttcatgctc accttttggc gaactggttt taactgagat
     1021 ccccggtagg ctgagtcctt catcagaaag caatgccagc gcacgggcat tcagcgtttc
     1081 tggatcaccc ggcaaaacga tttcaatatg ttcccaacct tcgtgcgggt aacgtttttc
     1141 cctaggccac ggtagctcca caatagaaaa ctgccagtgt gcaatctgta ccggttcatg
     1201 caatttaaac agacaaatcg gtctgccatt gatcatattt tctgacaaaa gctcgccaca
     1261 ctgttcaaac ccgcgacgcc agcgctcagc agtggcgttt tgatggcaac gcaaagaaat
     1321 gtgatcggca gtcagcggag tgatattcaa ccccagacgg cgggaaagtt catctaatgc
     1381 gtgggtaaat cgcggtaaat ccgatgcaat atcctgcagc tcgtcgatag attgccagtt
     1441 cgccataatc actcttcgtc tttcagtaaa agcgttaatt taccctgttg ccctgcgcca
     1501 accaaccgct gatttcacgc cgcttctgat gcaatagtga aaacggcaat acgccacgcg
     1561 cacgttgctg acgaaaacag ccatttgcag tatactcccg ccctaatttc tttaactggt
     1621 gcgggcaatt tttgctcgct tcatcaatgt aaggtattcc ggtgaatatt caggctcttc
     1681 tctcagaaaa agtccgtcag gccatgattg cggcaggcgc gcctgcggat tgcgaaccgc
     1741 aggttcgtca gtcagcaaaa gttcagttcg gcgactatca ggctaacggc atgatggcag
     1801 ttgctaaaaa actgggtatg gcaccgcgac aattagcaga gcaggtgctg actcatctgg
     1861 atcttaacgg tatcgccagc aaagttgaga tcgccggtcc aggctttatc aacattttcc
     1921 ttgatccggc attcctggct gaacatgttc agcaggcgct ggcgtccgat cgtctcggtg
     1981 ttgctacgcc agaaaaacag accattgtgg ttgactactc tgcgccaaac gtggcgaaag
     2041 agatgcacgt cggtcacctg cgctctacca ttattggtga cgcagcagtg cgtactctgg
     2101 agttcctcgg tcacaaagtg attcgcgcaa accacgtcgg cgactggggc actcagttcg
     2161 gtatgctgat tgcatggctg gaaaagcagc agcaggaaaa cgccggtgaa atggagctgg
     2221 ctgaccttga aggtttctac cgcgatgcga aaaagcatta cgacgaagat gaagagtttg
     2281 ccgagcgtgc acgtaactac gtggtaaaac tgcaaagcgg tgacgaatat ttccgcgaga
     2341 tgtggcgcaa actggtcgac atcaccatga cgcagaacca gatcacttac gatcgtctca
     2401 acgtgacgct gacccatgat gacgtgatgg gcgaaagcct ctacaaccca atgctgccag
     2461 gaattgtggc ggatctcaaa gccaaaggtc tggcagtaga aagcgaaggg gcgaccgtcg
     2521 tattccttga tgagtttaaa aacaaggaag gcgaaccgat gggcgtgatc attcagaaga
     2581 aagatggcgg ctatctctac accaccactg atatcgcctg tgcgaaatat cgttatgaaa
     2641 cactgcatgc cgatcgcgtg ctgtattaca tcgactcccg tcagcatcaa cacctgatgc
     2701 aggcatgggc tatcgtccgt aaagcaggct atgtaccgga atccgtaccg ctggaacacc
     2761 acatgttcgg catgatgctg ggtaaagacg gcaaaccgtt caaaacccgc gcgggtggta
     2821 cagtgaaact ggccgatctg ctggatgaag ccctggaacg tgcacgccgt ctggtggcag
     2881 aaaagaaccc ggatatgcca gccgacgagc tggaaaaact ggctaacgcg gttggtattg
     2941 gtgcggtgaa atatgcggat ctctccaaaa accgcaccac ggactacatc ttcgactggg
     3001 acaacatgct ggcgtttgag ggtaataccg cgccatacat gcagtatgca tacacgcgtg
     3061 tattgtccgt gttccgtaaa gcagaaattg acgaagagca actggctgca gctccggtta
     3121 tcatccgtga agatcgtgaa gcgcaactgg cagctcgcct gctgcagttt gaagaaaccc
     3181 tcaccgtggt tgcccgtgaa ggcacgccgc atgtaatgtg tgcttacctg tacgatctgg
     3241 ccggtctgtt ctctggcttc tacgagcact gcccgatcct cagcgcagaa aacgaagaag
     3301 tgcgtaacag ccgtctgaaa ctggcacaac tgacagcgaa gacgctgaag ctgggtctgg
     3361 atacgctggg tattgagact gtagagcgta tgtaatcgat ttttcgtgag agtgaagcct
     3421 gatcaggttt agccgatcag gcttttttat taccatctaa atgtattctg aaaataggca
     3481 tgccattgtt ttctcgctgt tgagtaagag gccagaagcg taatatccag ctccagggaa
     3541 acggtaacgg ttcaatttaa ggaataccgc agtgtttaaa tttcttgtat taacattagg
     3601 cattatctct tgccaggttt acgcagaaga tacgcttata gtaaatgatc atgacatttc
     3661 agccatcaaa gattgttggc aaaaaaattc aggtgatgat actgacatta acgtgatcaa
     3721 atcatgtctg cgacaagaat acaatctcgt cgatgcgcaa ttaaataaag cttatggtga
     3781 agcttatcgt tatatagaac aagtgccacg cacaggtgca aaaaaacctg atactgaaca
     3841 acttaacttg cttaaaaaat cacaacgagc ctggctggat tttagggaca aagaatgtga
     3901 attaatcctt tcaaatgagg acgttcagga tttaagtaac ccttattctg aatcagaatg
     3961 gctatcatgt atgatcatac agaccaatac gcgaactcgc cagttgcagc tataccgtaa
     4021 ctctgaagat ttttatccaa gccctttgac aagaggataa ttcacatatt tttggcatgt
     4081 tttgttgcaa gctattcctg ataaatgatt gcaacaaaac atccagcctt tttcactgag
     4141 ttattaaaca tactcgcgag cgcgtaattt ttttgtcctt cagcgataat tcacaatcac
     4201 ttcattacgt tgtactttca gcgacggaat taaccgccca ccgccaggca cttcccagat
     4261 aaagcgcaac ggttctgctg ccgctatacc ggaaaaggcc acggtggttc cgctctgccc
     4321 atctaattcg acacaacgag attgcgaaca caagcgaacc cgcagtcctg caggtgtcgg
     4381 gccgataagc tgataccgcc acgccaccag cgtcatcaat cctgaagcag gttgtcgcgt
     4441 agaaagaggt gcagacgaca tcgactcacc gcgatgattt agcgtaattc cgacactact
     4501 tgcctgccac atcccctccc cggcggcttg caccagcagc ggaaacaata atattgctaa
     4561 taaggttctc atcatttacc gccaattgtc gccgtcatgc ggatatgtcg gttatcagac
     4621 agttccagat tcgacagcac cactaactgc ggcaagctgc ggcgcagaaa gcgagacaat
     4681 aatggtcgca gcgcgtggtt caccaataaa actggcggcg cacccaacat ctcctgacgg
     4741 gatagcgctt cctgagtttg cgccagtaaa cgatccgcca gccctggctc cagtccgccc
     4801 ccgccctgca acgcctgtag caacaaacgt tccagcggtg tatcgaggcc aataacatgg
     4861 acttcatctt tgccaggaaa ccactgctgg gtaatcgccc gtcccaacgc cacgcgcacg
     4921 acggcggtta attcatgtgg atcgctttgg atgggcgcat gttccgccag cgtttcgaga
     4981 atagtgcgca tatcgcgaat cggtactttt tcatcgagga gattttgcag cactttatgc
     5041 agtgtggtga gcgtgacgac gccagggacg agatcttccg tcagctttgg catctcctgg
     5101 gcgacgcgat ccagcagctg ttgcgcctcc tgacgaccaa acagctctgc ggcatgctgg
     5161 ctaatgaggt ggttaagatg cgttgctacc accgtgctgg cctcaaccac tgtgtacccc
     5221 tgaatctgcg cctgttcttt tagcgcactt tcaatccaga tagcattcag gccaaatgcc
     5281 ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg ttccagggtt aatagccagc
     5341 cagcgccccg gataagcatc accactgcct atctccacgc ctttcatcaa aatgcgatag
     5401 cgggcaggtt gcagatccat attgtcgcga atgtgcacca ctggcggcag aaatcccatc
     5461 tcctgggcaa atttcttgcg gatactgcgt atacggccca acaactcacc atcctgctgg
     5521 aaatcgacca tcgggatcag tcgataaccc acttccattc ccagagaatc ttccagttgt
     5581 acatcgttcc acgtcgcttc gacaacggca ttattctctg ccatttttac cggttttggt
     5641 tcggcaggcg ctttttgttc gcgtccacgg atccaccagg ccagccccag caatccggca
     5701 gtgaacagca aaaataccag gttcggcatt ccaggcacca ggccgagtaa accgagcacg
     5761 gcagcgctta acaacataac gcttgggtta ctgaaaagct gattcaccat ctgctcgcca
     5821 acatcctgat cggtgctgac acgcgtaaca atgacccccg cggcggtaga aatcaccaga
     5881 gccgggattt gtgccaccag accgtcacca atggtcaaga gcgtataact ttccgccgcg
     5941 tgtcccatgc tcatgccatg ttgcagcacg ccgaccagca acccgccgac aacgttaatg
     6001 accatgatga ggatcccggc gatggcatcg ccgcgaacaa acttacttgc cccgtccatt
     6061 gagccgtaaa aatcggcttc ctgagtgact tcggagcggc gttttttcgc ctcatcttca
     6121 ccaatcaatc cggcgttaag gtcggcgtca atcgccatct gcttacccgg cataccatcg
     6181 agaacaaagc gcgcacccac ttctgcgata cgcccggcac ctttggtaat gaccataaag
     6241 ttgatgatca cgagaatgac aaacaccacg ataccgatag cgaaattgcc accaacgagg
     6301 aagtgaccga acgcttcaac caccttccct gccgccgctg cgccggtatg cccttccatt
     6361 aaaatgatac gggttgaagc cacgttaagt gccagacgca acagcgtggt aaacaacaaa
     6421 atggtcggaa acgcagcaaa ctcaagcgtg cgctgggtaa acatcgccac cagcaacacc
     6481 atgatcgaca aggcaatatt gaaggtaaac aacaggtcga gtatgaatgc gggcagtggc
     6541 agcaccatca tcgacaagat caacaggatc aaaatcggtc cggcaaggat ctgccattgt
     6601 gtcgatttca ggtttgtggg caggcgcagc atcgcggcca gattactcat gggtcggttt
     6661 ctcgttaata aaatctaggg cttccggcac cggaagatga gtaggttgta cagggcgctg
     6721 tccaccagcc agacgccagc gtttcagttg ccagacccag gccagcactt ccgccaccgc
     6781 ggcgtacagt tgacccggga tttgttgacc aatctccaca tgtcgataca gcgctcgcgc
     6841 cagcggcggc gcttcaagcg tcgggacgtt attttcagcg ccaatttcac gaatgcgcag
     6901 cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg ctcattttgt tttcgtcata
     6961 ctgcaacgct accgaatagt gggtcgggtt attgacaatg acatccgctt tcggcacatc
     7021 ggccatcatc cgacgccgtg cggcagctcg ctgcatctga cggatccgcc ctttaacatg
     7081 ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc tgccgcgaca ttcgcagctt
     7141 tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc accattggaa tgacaccaag
     7201 caccaccagc agtgcgcata gccctaccaa atccatcgca ttacccatgg cggtaatcgg
     7261 agactcggcc attaagcgca tcatatgcgg ccagtgatgc cagagaaaaa accccgtcac
     7321 gctgccaacc aggatggttt tcaaaattgc tttaagcaac tccgcgccag tctgtgccga
     7381 gaacatccgt tttatgcccg gtagcgggtt gagtttggaa aacttcggct gcaaggattt
     7441 tccgctaaat accagccccc ccagcataac cggagaaatg agcgccacca gcaccacgcc
     7501 gctaatcagc ggtagcagcg ccagcatggc ttctctgatc agcagaataa tttgcccgag
     7561 gatcagattc gggtctttga taatactgtg atcaaaatgc agcccagcgg agagcatgcc
     7621 cgacaatcga cgggccagcg acacaccgcc aaaccagata acactaacgc ccaccagcaa
     7681 aatcagcagt gaggtcagtt cacgggaacg cgggatttgc ccctcttccc gcgctttttc
     7741 tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat
     7801 cctggaaaga gtattaagcg taaatgatgc cagagcgcaa agcgttcaat ggtttgagta
     7861 aggggcaaaa caggcgggat ttagggcttt tgctgccaca catcaagcat agtgtgcgtt
     7921 tgtcggatgc ggcgtcatcg ccttatcaga ccgcctgata tgacgtggtc acgccacatc
     7981 aggcaataca aatcaaaatc caagactatc caacaaatcg tccacctgat cctgactggc
     8041 taccacaccg gctttgctgg tatcgacctg aggtccatta agcaaactct ggttttcacg
     8101 ttttggacgc gactcctgtt ccgggatgtt ttccaacagc accatcagca actggcgttc
     8161 gatctcctga atgacatcca tcatccgctt aatgacctgc ccggtgagat cctgaaaatc
     8221 ctgcgccatc atgatttcca gcagttgcgc gttagtaaag ctggtatgcg cgggtacatc
     8281 tgccagaaat ttccgcgtat ctgttaccag ttcacgggcg tcggcaaggt caatcggatc
     8341 ggcaaaccag tcatcccaac gttgggttaa cgcttttgct gatttctcca tttgatcctg
     8401 atgcggttgt gacgcctcaa cactgttcag cgcccgctcc gcagcctggg cggtcatctg
     8461 cacaacatag tacaaacgat cgcgcgcatc ggggatggct tccgccgctt cggcaatggc
     8521 ctgatccagt cccagttccc gcaaactgtc gcgcagcata cgcgtcaggc tgccgatgcg
     8581 cgcaatgata tcgccagctg aatgctcgtc agcaggtttg attgatggtt gcatcatagt
     8641 cgcatcctca catgcccagt ttctcaaaga ttttgttgag tttttcctcc agcgtcgcgg
     8701 cggtaaatgg cttcaccaca tagccactgg cccccgcttg cgccgcagca atgatgttct
     8761 ctttcttcgc ttctgcagtc accattaaca ctggcaatgc cgacatcgcg ccatccgcac
     8821 gaatcgtttt cagcaattcc aggccatcca tgttgggcat gttccagtcg gagataacaa
     8881 atccataacc gcctgcctgc aacttattga gagcgtcgac gccatcttcc gcttcctcaa
     8941 cattattgaa tcccagctct ttcagcaggt tacgcactat gcgtcgcatg gtggaaaagt
     9001 catccacaac caaaaattta agttctttat ccgccatttc acactcctga tttaaatacg
     9061 tatcgcctgt ccggcactaa tttttgccaa catttgctgg cttacctggt taagatcgac
     9121 cacttcgcag acaccaccca tattgatggc ctcgcgcggc atgccgaaca ccacgcaact
     9181 tgcttcgttt tgcgcaaggg tccatgcccc agcctgacgc atcgccaaca ttcccgccgc
     9241 gccgtcgttg cccataccgg tcaggatcac cccaaccgca ttacgccccg cctgtttggc
     9301 gacagaatgg aacaacacat ctaccgaagg ccgatgacgg ttaaccgccg ggccatcgtg
     9361 aattttgatt tggtaatttg cgccactacg cgccagctcc atatgccgat cgcccggcgc
     9421 aatataggca tgccccggca aaacacgttc cccgtcttcg gcttctttaa ccccgatctg
     9481 gcaaagctta ttaagtctgt cggcaaaaga gcgggtgaaa ccgggcggca tatgctgggt
     9541 aattaacagt gccgggctgg aaagcggcaa cggttgcagt acgtgacgaa ttgcctcagt
     9601 tccacccgtt gaagcaccaa tcgcaatcag tttttcagaa ctcaacaacg gccctgcctt
     9661 cagcgttgtc ggtgccgaca atggcttatg tgctgcaagg ctcgcctttg ctgctgtacg
     9721 caccttttca gcaatcattt cgctgtacgc cagcattcct tcgcgaatac ccagttgcgg
     9781 tttggtgaca aaatctattg cccccagctc cagcgcgcgc agcgtgactt ctgatccttt
     9841 gccggtcagg gaagaaacca tcacaacggg cattggacgc aaacgcatta atttttcgag
     9901 gaaatccagt ccgtccatcc gcggcatttc aacatccagc gtcagcacat cgggattgaa
     9961 tttcttaatc aagtcacgcg cgaccagcgg atcaggcgcg gtcgccacca tttccatgtc
    10021 gctatggctg ttgatgattt ctgtcatgat ctggcgcatc agtgccgaat catcgacaga
    10081 taacaccctg attttgctca tcgttaatcc ttacttagcg catacaccgt ttgaccacgc
    10141 agcgtgaagc ggcgctcgag gtggctaaag ttttcagagt gacccgcaaa cagcaatccg
    10201 tcgggtttaa ggagcggaac aaagcggcgc aaaatctcct gctgggtagt ttgatcgaag
    10261 tagatcatga cgttacgaca gaagatcgca tcaaacggcc ccggcacggt gtactgtttc
    10321 gccagtagat tcagcgaggc aaaatcaaca tagttcgcca gctcctgacg cacgcgtacc
    10381 agcccttcat gcggtcccgt ccctcgcatg aaataccgtt gcagttgctg cggcgtcagg
    10441 tttttcaact cttcatggcg atagataccg cttctggctt tttccagcac ttcggtgtcg
    10501 atatcactgg caaacacttt ccagcgtccg ggcgcggtgc ccaatgtgtc agccagcgtc
    10561 atcgcaatgc tgtacggctc ttcgccggtc gaagccgccg cgctccatac gcgatactcg
    10621 ccagaacggc gacgtgcgtg atccgcgagc agagggaaat gatgtgcctc acggaaaaat
    10681 gccgtcagat tcgtggtcag cgaattgata aacgcctgcc actcaccgct gtgctgatta
    10741 gattccagca agttcagata atgaccgaaa tccgtcagtc ccagcgaacg caaacgacga
    10801 accagtcggt tgtaaaccat gtcgcgttta tggtcagcca gaacgatccc ggctcgttga
    10861 tagatcaatt gacttatccg ccgaaaatgc gcgtcggaaa gcgccaggcg ctcggtcatc
    10921 tgtaacaata aagacgtttg cccacagggc agagatgaag tcatagcgcc ttctcaatca
    10981 cttcaggata ccactggcgc aatttgtaac tgcgccgact catgtcgtgc tacttcatgt
    11041 tcttcaaggg taaataccgc cacacgcgac gaaagatggt cggcctgatt cgccagttgt
    11101 tccgttgcca ccgccgcttc ttctaccagc gaggcgttct gctgtgtcac ctgatccatc
    11161 tggctgacag cctgcgcaac ctgctcaatc ccccgccgtt gttcttccga ggccgaagca
    11221 atttctccca taatgtcgtt cacgcgggtc accgaactga caatatcggt catggtcgcg
    11281 gcggcgttat tcaccagttt cgaaccctgc tggacacgat tgactgactc ttcgatcagc
    11341 cctttgatct cttttgccgc ctgggcgctt cggctggcaa gattgcgcac ttctcctgcc
    11401 actaccgcaa aaccacgccc ctgctctccg gcgcgagccg cttctactgc cgcattcagg
    11461 gccagaatat tggtctggaa agcaattccg tcgataacgc tgataatgtc gccaattttt
    11521 tgcgagctgg tggcgatctc ctgcatggtg tgagtcatgg tactgacctg aacaccgccc
    11581 gcctgcgccg ttgtcgcggc attttttgca agttccgacg cctgtcgtgc gttatcggcg
    11641 ttttgcccta ccgtggcggt taattgctcc atactggcgg ccgtttgtgc cagcgatgcc
    11701 gcctgctgtt cggtacgact tgagagatcg ttattgcctg cgacaatctc cgcgatacca
    11761 atgtgcatct cctggcttcc cttacgcaca tcacttaccg tcccacgcaa agcctgctgc
    11821 atggtcttca ggctggcaaa aatggcggtg atctcattac gaccatatac cgcaatcgga
    11881 cgcgccagat tacccgcagc aatgctgtca aaatggctac cgataatggc cagtggttga
    11941 acaatcatct tgcgcgtcca ccacagcgca ctgctgatgt agatcgccgc aacaataatc
    12001 atgctgataa acaccagcgc cgaaatctga tagttacgct ggctttgcgc actggcggct
    12061 tccagcacat ggatgatctc cagctgccag gcctcaaagt taacgtcaaa cgcattctgt
    12121 gatccctgca ccggcgcagt gaggaaatcc gaaagttgat tactttcaag ccaggtcgcc
    12181 tgatgttcga gatcgttgtg ccagcgggca aaacttttct ccgtctcttt ttgcaatgcc
    12241 ctgacgtgct cgttgcccgc agtcatcgcc ataaaacttt taaacagcgt ggtggattgc
    12301 gtcagactgg cacgcgccgt cgtcatcaac gttttaatgt catccgccgg atagctaagc
    12361 gctgtcagag tgcctgcttt gttcagcgtg gtactggcct gtaacattac cgcccgagtt
    12421 tgcgctaatg ccgcacgttg ctgattgctc tgctcgacct gattcagtcg ttgcaaatcg
    12481 tcgcgaaatg cccaaaaaga catgccgtta ctgccaatct gcaagatccc gcagagaatc
    12541 aaaattaaaa acagcgtggt cgaaattcga atacgattaa acatcaacgc tccccatcag
    12601 gcggcaatga ccgcgttagt aaatactcgt caaaatgttt cccagtttgg atcttgttca
    12661 gcaattcgca gtcgtggctg tgccggtggt tgctcactgg caggacggga tggtgtttgc
    12721 ggtttattgg tgagtgggct ggctgccaga cgaaacgcgg aaaccgcttg cgttaaacga
    12781 ctagcctgtt cttccagcgc agctgcagcg gcagctgatt cctgcaccag cgatgcgttc
    12841 tgttgcgtga cgtgatccat ttccgaaacc gccaatgcga cttgatcaat gccacggctc
    12901 tgttcatccg atgccgatgc aatctcgccc ataatgtcag tcacgcgagt gacagcatcg
    12961 acgatattgt tcattgtttc cccggcgctt tcgaccagca ccgaaccggt atcaacgcgc
    13021 gagacggagt cttcaatgag agctttgatc tcttttgccg cctgggcgct acgactggca
    13081 agattacgca cttcgcccgc caccacggca aaaccacggc cctgttcacc cgctcgcgcg
    13141 gcttcaacag cggcattcag cgcgaggata ttagtctgga aggcaatacc gtcgattacg
    13201 ctgataatgt cggcgatttt cttcgaacta tcggcgatct catgcatcgt tttcactacg
    13261 ccatccacca ctttgccgcc gtgctgggcg gtgtcggagg cactttgcgc cagttgcgag
    13321 gcctggcggg cgttatcggc gttttgcttc actgtcgcgg tgagctgctc catgctggcg
    13381 gcagtttctt ccagcgcgga tgcctgctgt tccgtacggg aggaaagatc ggtgttgccc
    13441 gccgcaattt cacgggtacc ggcatagatg gcatctgaac cttcgcggac atgagtgacg
    13501 gtgtcagtca aagagcgttg catatgtgaa acgctctgcg ccaggtcgcc catttcactg
    13561 cgcccgtcaa tggtcagggt attcgccagg ttaccaccgg cgatttcgcg aatgtgagca
    13621 ataatttttg ccagcggtgt aagcaacata cggcgaatgc cataccacgc caccagcaga
    13681 atcaatacca ccaccagcgc gataaccgcc agttgccact gggcaaatcg gtaatcatct
    13741 gcgttgtcag tgacgatatc gcgatacagt ttttcactgc tgagggcgta ctgagcaaac
    13801 gcttcgccca ttgcattttg cattccctgg gttggctgag cgaaataagc tccagtattg
    13861 ccataatcaa gataatcaat cagttcagtt aacgctgtgt aatagttttt atatttttca
    13921 tcaatattac gactggtagc gaccatttca ggtaacggtg ccatgctttt gaatttttta
    13981 taatgagtcg cggcctgggc caatgttttc ctggcgctat cgagcaattc aactttggca
    14041 ttactttgtt gattggagga atccatcatc attcgcaccg ctgaacgact caggttaatg
    14101 cgcgtttgca gcattaaatc ccaggttgac gtcagctcgc cctgctgttc tcgtaattga
    14161 ttggaaacca caaagctctt ctggctatgg tgaagggaag aaaaaaacag actgccggaa
    14221 ataagctgta acagtgcgaa tacccccagc accattacca acagcgtgac tacgcggata
    14281 cggttaatca taaggcacct tcctgaaaac aagttgatct cgttatcggc aaggaagggg
    14341 gaaactttat tgctgatgcc acccgccgcg aaattgaaat aaaacccgat gcgcagatca
    14401 tcgggttcat ttcaattgag gaaatcagga gaattacgcc acttctgacg ccgcgctatc
    14461 taacagcgcc atctcttcgc tgttcagcag tttttcgatg ttcaccagaa tcaacatacg
    14521 gtcgcccagt gcgcccagtc cagtgagata ttctgttgaa agcgtcacgg caaactctgg
    14581 tgccggacga atttgctccg ccgtcaatga aagcacgtct gaaacgccgt caaccacgat
    14641 gccgaccacc cgctgtccga gattcaggac gataactacc gtgttgtcgt tatagtccac
    14701 atccacctgg ctgaacttaa ttcgtaagtc aacaatcggc acaataacgc cgcgcagatt
    14761 cgtgacgcct ttgataaacg ctggcgtgtt cgcaatccgt gttacctgat cgtagccacg
    14821 gatctcctgc actttcagga tatcaatacc gtactcttca tcaccaaggg taaataccag
    14881 aaattcctgg cctgacggct cgctggccag ctttgttaca ttcgtcatac cggtcatatt
    14941 gttacctttt tactcattca ggcggcggtg ttcgccatac gttgttcgcg gtttatcgcc
    15001 tgcaaggcgg agacatcaac aatcagtgcc acgctgccgt cgccaagaat ggtcgcagca
    15061 gaaatgccgg ggactttgcg atagttactt tcaaggtttt taactacaac ctgatgttga
    15121 ccaattaatt gatccaccag caaggcgtag cggcgaccgc cactttgtaa gatcaccaca
    15181 attccctggg tagcttcggt tttcgcgccc gcgacgttga acactttcca cagttcgacg
    15241 atgggcagat attcaccccg cacttccagc acccgctcgc cgccggccag tggatgcaga
    15301 tcggcttcac ggggttgcag tgattccata acagcattca gcggcagaat gaatacttca
    15361 tccgcaacgc gtacggacat gccgtcgagg atggccagcg tcagcggcag taaaatgcgg
    15421 atcgtagtgc cagtaccctg cttcgactgg atttcaacat gaccgcccat ctcctggata
    15481 ttacgtttaa cgacgtccat gccgacgccg cgcccggaga cgtcggtgac ctgctctgcc
    15541 gtggagaagc ctggtgcaaa tatcagcatc gcgacttcgt cgtcgctcat gttttcgctg
    15601 acagtcaaac cttgcgaggc cgcttttgcc agaattcgct cacggtttag ccccgcccca
    15661 tcgtcggtca cttcaatgca aatgttgccg ccctgatgtt cggcagacag aattaaattt
    15721 ccgacgctgt ttttacctgc ggcgagccgt ttttctggca gttcaatacc gtgatcgagg
    15781 ctattgcgta ccaggtgggt cagcgggtcg ataatgcgtt ctatcaggct cttgtcgagc
    15841 tcggtggaac tgcccaccag cgtcagttct acctgcttgc cgagttttcc cgccagatca
    15901 cgcaccagcc ggggatagcg actaaagaca tattccatcg gcatcatgcg aatcgacatc
    15961 actgattcct gcaaatcacg ggcgttacgt tgtaactgcc ccatgctggt aatcaaatca
    16021 ccatgattaa ccgggtccag ttcgctggaa cgctgggcaa gcatggactg ggtgataacc
    16081 agctcgccga cgaggttaat taattgatca accttttcta ccgctacacg gatgctggtg
    16141 gattcactgc tacgcgtcgt tttttcccgc tccacgcgac cggttggcgc ttgttcggct
    16201 gccagtttaa gcactggtgg ggtggatatt tttggcgaga cttctactgt ttcaaaggta
    16261 atctgatcgg cttcaatcac aaaacagagt accgctgtga tgtcatcttc ggcgatatcg
    16321 cccggtaata ttgccgagag agaatccgcc cctttcacca cgtcagttaa cgttgtcaga
    16381 tgccccagct cttcttccag caggtcgact tcgctggcct tcaggcgcga aaggataatt
    16441 cgtcgcggca actgactgcg actctgctca tcttgcggtt cacttttggc aaccacactt
    16501 aatcgggtca ctgcggatgg cgtttcgcct ttcgcttcta atgccagttg acgcaaggcc
    16561 tggcagatat aatcgaagct ggcggcatcc ggctcttgcg actgtttata agcgtcgagc
    16621 tgttcttgca tgatgtcctt cgtttccaaa aacagattga taatgtcggt gttgagttgc
    16681 atctcacctc gtctggcttc atcgagcagg ttttccatca gatgcgtggt ttcctgcaaa
    16741 acgctgaagc caaaagttcc tgcccctcct ttgatcgagt gggcagcccg aaagatggca
    16801 ttcaattgtt cggcatctgg cgcttccggc tgcaaaacca gcagatgctg ctccatatca
    16861 gccaacagtt cgtccgcttc atcaaaaaat gtctgataaa aatcgcttat atccatgctc
    16921 acgctgtcac ctcggttcgg ctgatggcat tgtgggaaca ctgacctgtg gtgcaacctc
    16981 aggtttttcc agggcgctta ctggctcatt ctggctttcg gcgttttcat gcaaaatggc
    17041 ctgttcggct tgtttgttca gtaccagcag gctgatgcga cggttgacgg catcatcagg
    17101 tccgcgatcg cttaagcgca tcgttgccgc catgccgacc acacgtaaca ctttgccgct
    17161 atccaaccct ccgaccatca gttcgcggcg ggatgcattg gcccgatcgg cagaaagctc
    17221 ccagttgctg taacctttct caccactggc gtaggggaaa tcatcggtat gacccgaaag
    17281 gctaatacgg ttgggaatac cattcagaac gggcgcgatg gcacgcagaa tgtcgcgcat
    17341 atagggttcg acgtcggcac tgccagttct aaacatcggg cgattctggc tatcgatgat
    17401 ctgaatacgt agaccttcct ggaccagatc gattttgaga tgtggacgta acgcccgcag
    17461 tttcggatcg gactctatca attggtcgag atcgccacgc aatttccgca atcgactttg
    17521 ctccatgcgt tttttcaact cttcgatgtt cggctgctta ttcacttccc cctggctttg
    17581 ggtgtaatca tcgccaccgc cgggaattgg gctttcacta ttagaaatgc gatcgccgcc
    17641 cgtaaccgca gtcgccagtg gagtccggaa gtactccgca atctgaatca actcttttgg
    17701 gctggagatg gagatcagcc acatcaccag aaaaaaggcc atcatcgcgg tcataaagtc
    17761 ggcataagca atcttccacg atccatgtgc tgccccgtgg cttttggctt tgcgtcgttt
    17821 gacgacaata atcggatgcg cttgattctt catgcttcct cggttgtcgt ctgttgttgc
    17881 ggatttttca ccgcacgcac atgctcttcc agttcaataa acgacggacg ttcgctggaa
    17941 tagagcgttt tgcgaccaaa ctcaacggcg ataggcggtg cgtaaccgtt cagattagaa
    18001 agcagtgtga ctttgacgca ctgcatcatt ttgctggttt cggcgctttt ctgacgtaaa
    18061 acactcgcta atggggaaat aaatccgtaa gccaataaaa tgccgaggaa agtccccacc
    18121 atcgcatgtg cgataagcgc ccccagctcg gcggcaggac gatcggctga acctaacgcg
    18181 tgaacgaccc ccattacagc cgcgacaata ccaaacgccg gaagtgagtc cccgaccagc
    18241 gccagactgt tcgccgggac ttctgcctcg ctttcgtgcg tctcaatctc ttcatccatc
    18301 agggcttcga tttcgaaggt gttcatgtga ccgctgataa tcaggcgcag ataatcgacg
    18361 ataaaatcaa gcatgacgct atccgcgatg atgcgtgggt agctggcgaa gatctcgctc
    18421 tcacggggat tttcaatatc acgttccagc gaaaacatcc ccatctgccg cgatttcgcc
    18481 atcaaccgat aaagcagagc cagcagatcc atatacattg ctttggtgta tttggagcga
    18541 cgaaacagca acggcagcgc tttcagcgtg cctttaatcg cttttccatt attgccgaca
    18601 ataaacgacc caatccctgc accggcaata atcaccagtt cagcgggttg atagagtgct
    18661 ccaaggcttc caccggtcat caaataaccg ccgaaaactg taccgagaac aaccaggtaa
    18721 cctaataaga taagcacgac atcatccttc cactgttgac catgacagga tgttcagtcg
    18781 tcaggcgtta acgcgcgatt ggggcaaaaa aaagcagcgg tacgtcgtta ccgctgctgg
    18841 aatgttgcgc cacaccgtat cagttaaaca gcctgtactc tctgttcatc cagcagttgt
    18901 gggataatat cggcaggatt ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt
    18961 tgacataagc tgcaggcaaa gctgccaaca ggctggtgag cgtgggtaat aaaattgccg
    19021 ccgcagcagt tgcagctgga aagttgcagt aatccacttt caacaaaccg caccaatgtc
    19081 caggcacggg ttaatgccag cagcggtcct tcttctgctt gcgggcactg ttcaaggtat
    19141 aaacggtagg ctttgatcac cgcatcgacg ccattacaca aaccggtttt cagtaaaaac
    19201 tgccatgcat tacagaacat cgaagcatga acgttttgtt cccaagtcat aaaccagtcg
    19261 gttgagaatg gcagcatgcc tttcggcggt gggcttccgc gcagttcttt ataaagtttt
    19321 atcaggcgtc cgcgacttaa ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg
    19381 gtgatcaatt ccattgccag ctgaatatcc cgcgcttcct gaacaatgct tttttcactc
    19441 atgatcaggc ccttttcttg cgcagcgctt cttcaggctg attaacatca ttcagcaagc
    19501 gtgttgagag catgatgccg gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg
    19561 tcaacagagt aatcgtctgg tggctgtcaa aacggaagtg acaaaccagc tgattggttt
    19621 ctgccagctt aaccatttgc ggaagagtca gtgccgctaa tgttgtcgcc atttcttcat
    19681 ttatgccgag acgaaacata gcggacgctt tgtcctgaac aatcagacgc tgtgcaagta
    19741 gtaaatatga caagttgatg tcataaatgt gtttcagcaa ctcggaggta tgcattattc
    19801 ccacccagaa taaccaactt tatttttatg cggtttcacc gcaccccgtg atgtcgccgg
    19861 gaagccccgg taaaaaataa ttagcattag aatagttgcg ataagctgca ataagcagag
    19921 ccaccttttt ggtttaataa gtccttacaa atagaagggg gtctttacac ttatctaaga
    19981 tttttcctaa atcgacgcaa ctgtactcgt cactacacgc acatacaacg gaggggggct
    20041 gcgattttca ataatgtgtg atgcagatca cacaaaacac tcaattactt aacataaatg
    20101 tataagtcat acttttgttt tgggtgtatt tcaatctgtt aaaaagtttt tcgctacgct
    20161 agcaagcaaa aatgaaacag gaataatcga aatgggatgt tgcgcacagt taaaataact
    20221 caccgtaaat aatcatctgc tataaataat cactttcatg caatac
//
LOCUS       DAZFDC010000084        20186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFDC010000084 DAZFDC010000000
VERSION     DAZFDC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.084"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..85
                     /locus_tag="ACHJ48_003155"
     CDS             <1..85
                     /locus_tag="ACHJ48_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO4708656.1"
                     /translation="KINQLDSDNKLNINNDDIVAVGMTYQF"
     gene            complement(124..525)
                     /gene="crl"
                     /locus_tag="ACHJ48_003156"
     CDS             complement(124..525)
                     /gene="crl"
                     /locus_tag="ACHJ48_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO4708657.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(583..1827)
                     /gene="frsA"
                     /locus_tag="ACHJ48_003157"
     CDS             complement(583..1827)
                     /gene="frsA"
                     /locus_tag="ACHJ48_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4708658.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(1919..2377)
                     /gene="gpt"
                     /locus_tag="ACHJ48_003158"
     CDS             complement(1919..2377)
                     /gene="gpt"
                     /locus_tag="ACHJ48_003158"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4708659.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2638..4095
                     /gene="pepD"
                     /locus_tag="ACHJ48_003159"
     CDS             2638..4095
                     /gene="pepD"
                     /locus_tag="ACHJ48_003159"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4708660.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4152..4673)
                     /gene="prfH"
                     /locus_tag="ACHJ48_003160"
                     /pseudo
     CDS             complement(4152..4673)
                     /gene="prfH"
                     /locus_tag="ACHJ48_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(4672..4875)
                     /locus_tag="ACHJ48_003161"
                     /pseudo
     CDS             complement(4672..4875)
                     /locus_tag="ACHJ48_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(5194..5646)
                     /gene="yafP"
                     /locus_tag="ACHJ48_003162"
     CDS             complement(5194..5646)
                     /gene="yafP"
                     /locus_tag="ACHJ48_003162"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4708661.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(5656..6054)
                     /gene="yafO"
                     /locus_tag="ACHJ48_003163"
     CDS             complement(5656..6054)
                     /gene="yafO"
                     /locus_tag="ACHJ48_003163"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO4708662.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(6057..6350)
                     /gene="yafN"
                     /locus_tag="ACHJ48_003164"
     CDS             complement(6057..6350)
                     /gene="yafN"
                     /locus_tag="ACHJ48_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO4708663.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(6402..7457)
                     /gene="dinB"
                     /locus_tag="ACHJ48_003165"
     CDS             complement(6402..7457)
                     /gene="dinB"
                     /locus_tag="ACHJ48_003165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO4708664.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVSAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(7528..8313)
                     /gene="lafU"
                     /locus_tag="ACHJ48_003166"
     CDS             complement(7528..8313)
                     /gene="lafU"
                     /locus_tag="ACHJ48_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO4708665.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            8285..9997
                     /locus_tag="ACHJ48_003167"
                     /pseudo
     CDS             8285..9997
                     /locus_tag="ACHJ48_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(10142..10639)
                     /gene="rayT"
                     /locus_tag="ACHJ48_003168"
                     /pseudo
     CDS             complement(10142..10639)
                     /gene="rayT"
                     /locus_tag="ACHJ48_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414763.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
     gene            complement(10815..11564)
                     /gene="yafL"
                     /locus_tag="ACHJ48_003169"
     CDS             complement(10815..11564)
                     /gene="yafL"
                     /locus_tag="ACHJ48_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4708666.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQVSYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            11774..12034
                     /gene="dinJ"
                     /locus_tag="ACHJ48_003170"
     CDS             11774..12034
                     /gene="dinJ"
                     /locus_tag="ACHJ48_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO4708667.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGV"
     gene            12037..12313
                     /gene="yafQ"
                     /locus_tag="ACHJ48_003171"
                     /pseudo
     CDS             12037..12313
                     /gene="yafQ"
                     /locus_tag="ACHJ48_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
     gene            12469..13209
                     /gene="dpaA"
                     /locus_tag="ACHJ48_003172"
     CDS             12469..13209
                     /gene="dpaA"
                     /locus_tag="ACHJ48_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO4708668.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(13180..13947)
                     /gene="yafJ"
                     /locus_tag="ACHJ48_003173"
     CDS             complement(13180..13947)
                     /gene="yafJ"
                     /locus_tag="ACHJ48_003173"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO4708669.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGKTDSEKAFCWLLYKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14052..14630)
                     /gene="lpcA"
                     /locus_tag="ACHJ48_003174"
     CDS             complement(14052..14630)
                     /gene="lpcA"
                     /locus_tag="ACHJ48_003174"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO4708670.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            14870..17314
                     /gene="fadE"
                     /locus_tag="ACHJ48_003175"
     CDS             14870..17314
                     /gene="fadE"
                     /locus_tag="ACHJ48_003175"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO4708671.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTLGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNVFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(17357..17803)
                     /gene="ivy"
                     /locus_tag="ACHJ48_003176"
     CDS             complement(17357..17803)
                     /gene="ivy"
                     /locus_tag="ACHJ48_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO4708672.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            17984..18754
                     /gene="yafV"
                     /locus_tag="ACHJ48_003177"
     CDS             17984..18754
                     /gene="yafV"
                     /locus_tag="ACHJ48_003177"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO4708673.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLR"
     gene            complement(18872..20044)
                     /locus_tag="ACHJ48_003178"
     CDS             complement(18872..20044)
                     /locus_tag="ACHJ48_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
                     /protein_id="HFO4708674.1"
                     /translation="MSIQSLLDYISVIPDIRQQGKVKHKLSDILFLTVCAVIAGADEW
                     QEIEDFGHERLEWLKKYGDFDNGIPVDDTIARVVSNIDSLAFEKIFIEWMQECHEITD
                     GEIIAIDGKTIRGSFDKGKRKGAIHMVSAFSNENGVVLGQVKTEAKSNEITAIPELLN
                     LLDLKKNLITIDAMGCQKDIASKIKDKKADYLLAVKGNQGKLHHAFEEKFPVNVFSNY
                     KGDSFSTQEISHGRKETRLHIVSNVTPEFCDFEFEWKGLKKLCVALSFRQKKEDKSAE
                     GVSIRYYISSKDMDAKEFAHAIRAHWLIEHSLHWVLDVKMNEDASRIRRGNAAEIISG
                     IKKMALNLLRDCKDIKGGVKRKRKKVALNTCYIEEVLASCSELGFRTDKMKNLTQI"
     gene            20125..>20186
                     /locus_tag="ACHJ48_003179"
     CDS             20125..>20186
                     /locus_tag="ACHJ48_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708675.1"
                     /translation="MIYITIFMILPCPVPCSHVFL"
BASE COUNT         4924 a         5163 c         5027 g         5072 t
ORIGIN      
        1 taaaatcaac caactggata gcgataacaa attgaatatt aataatgatg atattgtcgc
       61 ggttggcatg acctatcagt tttaatgatt attgccggat gtgatgcatc cggcacattt
      121 cactcacgcc gttaacttca ccggctcgtc acgaaaatca tccgccggtt ccaacttcag
      181 attcagcgtc gtcagcagtt cacgcagctt ctcgtgaaac tcacgcaggg tgtgctccag
      241 tcgttcaacc acttcagtat cttttaccgg aacactcttc cagtcgcctg ctttatcgaa
      301 cagaccaaac tggtaactgt aggtaaaacg ggattcctgc gcttcaagct ccatccacca
      361 gccccagaac tcacgcactt ccggtgccgg tttcacgttg acgcatacag ccagacaatc
      421 gaaaaagaat cgattatctt cgcacttacc ttcacgaata tacgggccta gtacggtaaa
      481 ttttttgatc aatctgctct tcgggtgtcc actcggtaac gtcattgcta tctcctgttg
      541 tgatgcaact gttttaccaa attggcaaaa tttagcaaat ttttaacaca agcgtttttc
      601 gatccagtcg gtgatttcct gaagaccttt gtcaaaattc cgatacaccg ggttaaatgg
      661 gatctctaat aatttaccgt cagcagatga tgaggtgatt aagcgtgagt cctcttccgg
      721 gctgaacgga tcgttcttcc agtagcctga taacattggc gttgggcatc gacgtcccag
      781 caatccttgc acttttaatg aatagcgatt cagctccacg cgcaacgctt cgtcggaagc
      841 atcatgcatc cccaaacgac tcgccagaac gtcaagatac atttccggca cctgttgctg
      901 gcacttaaaa tcactcaaca gggtatgaac taccggacca agacaggcaa ccgctttcag
      961 acgcggcgat tcaagatatg ccagacgcac ggcaacgtta gcgccgaaac ggaaaccaaa
     1021 ggccgcgacg cgagtgtgat ccacccacgg tacgttaggc agagccttta agacgcgctg
     1081 atgcaacagg caggagtcct gggtgagctt ccattttgaa gaaaagccca ccgacggcat
     1141 atcaatagtc agcatcgcaa tgccgcgcgg cgcaaaataa cgttcataca ggctgtaata
     1201 gtccgtctgc atcgcatcca gaccaccaca cattaatacc gtcgggaacg ggccatcgcc
     1261 tttcggcata tgcaaaaagc cggtgatggg cgcaccgccg ggtacggtaa actccatctg
     1321 tcgcatcgtg cccggtagac gctgagcggc ctcttcatag gcgcggtttg acaaagcctg
     1381 cgcttgctcg gccaggtcat ctcctttcag atgaggatag gcggcaatgt tgtacaacgt
     1441 agccgcatgc agccagtgac gaccactgag ttgcggatct tcttccgcac aggctttttg
     1501 ttgccacacc atcgcctggg tggcccactc ataaatccag ttgccgccac ggtagccaat
     1561 caccgtatcg tataaatcat cgtcggtacg ttcggcatcg ctcatcacaa tacgtgcctg
     1621 gacgtcgagg atttcgcgtg ggtcaatgcc gcgccagatc cacatcagac ggttaatcat
     1681 gcgataccag tgagggatgg ttttaccatc aagggccgac tgcacaggcg gttgtgcgcc
     1741 gtgattaaag cggcggacta gcgtcgaggt ttcaggatgt ttaaagcggg gtttgaacag
     1801 ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc agaatactta ctggaaacta
     1861 ttgtaactcg cctgaagtta aaaagaacaa cgcccggcag tgccaggcgt tgaaaagatt
     1921 agcgaccgga gattggcggg acgaatacga cgcccatatc ccacggctgt tcgatccagg
     1981 tatcttgcgg gatatcaaca acataatcat caaccagcgg acgaccagcc ggttttgcaa
     2041 agatggtgac aaagtgcgct tttggataca tttcacgaat cgcaaccgca gtaccaccgg
     2101 tatccaccag gtcatcaata acgatgaagc cttcgccatc gccttctgcg cgtttcagca
     2161 ctttaagctc gcgctggttg tcgtgatcgt agctggaaat acaaacggta tcgacatgac
     2221 gaatacccag ttcacgcgcc agtaacgcac ccggtaccag accgccacgg cttacggcaa
     2281 taatgccttt ccattgttca gaaggcatca gtcggcttgc gagtttacgt gcatggatct
     2341 gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg aagtgtccca gcctgtttat
     2401 ctacggctta aaaagtgttc gaggggaaaa taggttgcgc gagattatag agatctggcg
     2461 cactaaaaac cagtatttca catgagtccg cgtcttttta cgcactgcct ctccctgacg
     2521 cgggataaag tggtattctc aaacatatct cgcaagcctg tcttgtgttg acaacatttt
     2581 ctgctaaccc tgtgacctgc aatactgttt tgcgggtgat cgacaaggag acttaacgtg
     2641 tctgaactgt ctcaattatc tccacagccg ctgtgggata tttttgccaa aatctgttct
     2701 attcctcacc cgtcctatca tgaagagcaa ctcgctgaat acattgttgg ttgggcaaaa
     2761 gagaaaggtt tccatgtcga acgcgatcag gtaggtaata tcctgattcg taaacctgct
     2821 accgcaggta tggaaaatcg taaaccggtc gtcttgcagg cccacctcga tatggtgccg
     2881 cagaaaaata acgacaccgt gcatgacttc acgaaagatc ctatccagcc atatattgat
     2941 ggcgaatggg ttaaagcgcg cggcaccacg ctgggtgcgg ataacggcat tggtatggcc
     3001 tctgcactgg cggttctggc tgacgaaaac gtggttcacg gcccgctgga agtgctgctg
     3061 accatgaccg aagaagccgg tatggacggt gcgttcggct tacagagcaa ctggttgcag
     3121 gctgatattc tgattaacac cgactccgaa gaagaaggtg aaatctacat gggttgcgcg
     3181 gggggtatcg acttcacctc caacctgcat ttagatcgtg aagcggttcc agctggtttt
     3241 gaaaccttca agttaacctt aaaaggtctg aaaggcggtc actccggcgg ggaaatccac
     3301 gttggcctgg gtaatgccaa caaactgctg gtgcgcttcc tggcgggtca tgcagaagaa
     3361 ctggacctgc gtcttattga tttcaacggc ggcacactgc gtaacgccat cccgcgtgaa
     3421 gcctttgcga ccattgctgt cgcagctgat aaagtcgacg ccctgaaatc tctggtgaat
     3481 acctatcagg agatcctgaa aaacgagctg gcagagaaag agaaaaatct ggccttgttg
     3541 ctggactctg tagcgaacga taaagctgcc ctgattgcga aatctcgcga tacctttatt
     3601 cgtctgctga acgccacccc gaacggtgtg atccgcaact ccgacgtggc aaaaggtgtg
     3661 gtcgaaacct ccctgaacgt cggtgtggtg accatgactg acaataacgt agaaattcac
     3721 tgtctgatcc gttctctgat cgacagcggt aaagactacg tggtgagcat gctggattcg
     3781 ctgggtaaac tggctggcgc gaaaaccgaa gcgaaaggcg catatcctgg ctggcagccg
     3841 gacgctaatt ctccggtgat gcatctggta cgtgaaacct atcagcgcct gttcaacaag
     3901 acgccgaaca tccagattat ccacgcgggc ctggaatgtg gtctgttcaa aaaaccgtat
     3961 ccggaaatgg acatggtttc tatcgggcca acaatcaccg gtccacactc tccggatgag
     4021 caagttcaca tcgaaagcgt aggtcattac tggacactgc tgactgaact gctgaaagaa
     4081 attccggcga agtaattatt tgatttgctg ccggatggcg tttaatcgcc ttccggcagt
     4141 ttcatccttc attatccttc gataaaagcc atccctgtga acgttcgtcg cgggttgcca
     4201 cgttcaatct ggtgatggaa cattcgccgc tgcgatttca gcactgcgct attttcctgt
     4261 tgctgctgct ccagcttcca ggcaatcagc aatcgcgcca gccgtttgtt agcatgctgg
     4321 ctgcgttctg actgaacctt cacgctaatc ccggttgcca gatgcgtggc gcgtaccgcc
     4381 gagtcggttt tattgacatg ttgaccgccc ggccccgacg gacgcagcgt ctcataacgg
     4441 attgcatccg attgttcctg ctcatcagcg gtaaaacgcc caatgcccag aaaccagttt
     4501 ttgcgcccat gatgaggccg atacggactc agacaaatcc actgaatagt gccgcaccaa
     4561 ctttcgctta acgcccatgc gttgtcgcca tccagagaaa tcagcgccga acgcagcgtg
     4621 tcagagtagc ggcccgtttc tgtttccagc accgttaccg cgacgtcttg tcgggtaaaa
     4681 acgaccaaca gagaagaacg ctgcgccaat cgggtagccg cgtccggggt gtaaatctgg
     4741 catccccact acgcgctgca tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc
     4801 taaactctcg atccacaggt catcagatgc gatggtaaat accgcatcgg ataagggacg
     4861 aatatatttg cccatgtacc attccattta tactcgtcat acttcaagtt gcatgtactg
     4921 cgactgcgtt cgttcacccc agtcacttac ttatgtaagc tcctggggat tcactcgctt
     4981 gtcgccttcc tgcaactcga attatttaga gtatataaat ataaaaatca ggaaatggca
     5041 gacgaatcca gggcaagaaa aaaaagaatt aatcctggaa aggtctgcaa agagtcattg
     5101 ataaaggatt tgtcatgatt tacctccttg cagtttgtgg aataagaaaa cgcgcacata
     5161 ccaggcgggc gttattttca ttgcaagctg gatttaatgt tgcggtttat atcgcataga
     5221 aaaattagta aaccacgctc cccggcattc aacgcgctgc tgcttaactg tctgaaaacc
     5281 ataacgttca aaaaagggtt ttgcggttat gctggcgtcc accgtaagtt cggattcaga
     5341 cttaatccaa ggttttaaca aagcgctggc aaccccacgg cgggtgtatt cagggtcaac
     5401 aaataacata tcgatataat gttcaatgcg ggaaataaaa ccaaccggtt gtgcattaat
     5461 gactgcaacc cgcacttgtg atttcgcgag tttctccttc cagcgagatt cgtcaatctg
     5521 cgcccaggcg gcaatttgtt gtggtgaata atgctgactg gcggtcatca taaccgctct
     5581 aatgaaaata gcgcatagtt gctgaaaatc gccaggctga tagtttctta tttgtatgtt
     5641 attcataata taaattcaaa aacgcatgcg aaacgcttct gccattttcc caattttatg
     5701 catttggttg ttatctcgag ccagtttatg aggttcaggt ttcagaatgg caatgagcaa
     5761 ccatgcttgc tcatcaaacg caccctgaca atataccaaa tgcgattcgt cattcgttct
     5821 gctgaattgg cgcaactgtg gcggaaatgg attattcaca tttgccagat gaatatgagc
     5881 aactcgctca aatttgatta atggccaagt aaacgagtcg tcgtagagtg catcgcgacc
     5941 aaatatatct ggcaaaacac cgtcacgctt ataggaaata aaatccgccg ttaacgcatc
     6001 aagctcctct gctgtaagtt gcaggcgaat aagttttgtt ttgaataccc gcatccttat
     6061 tccttaaagt cattgaaatc atcatccgtc atatcattaa gatatttttc agctcggcgt
     6121 gtaatttcct ctaatcttgc agcacttgga cggaagcgcg cctttatggg ctttttctcc
     6181 tcttgttcag caagcatgtc cattaacgct tcgaatgcgc tggcacttaa gagatatcct
     6241 gcggggcgat tattagaaag aaccgcaacc ggttgatcaa taaagtattt agctgggttt
     6301 ttacgtaatt cagtgatatt gaccgatttt tcagcgagaa ttcgatgcat acagtgatcc
     6361 cctcataata atgcacacca gaatatacat aatagtatac atcataatcc cagcaccagt
     6421 tgtctttcca tttgagggtc aagcaacgtc acatgcagcc ccaccagtcg cacaccgcgc
     6481 ccgccgcggc gttcatccca ggttttacgc gcggtggcga ttaagtcagc tttattcagc
     6541 cgcggccaga cgtgctcctg ggtggtttgc tgaaaatcgt cgaactttaa tttcaccccc
     6601 tggcgagcaa tcagtaaatc aggttttacc tttgccagac ggcgttcaag ttccggatac
     6661 agccgctcga taatcgcttc acattcagac cagtgatgaa tatcttccgc catcgtgcgt
     6721 tccacgccga cggattttcg taaccgttcg ctgttaacgt cgcgctcgtc aatcccctga
     6781 ctacgctccc acaaaatgcg gccaaatttg ccaaagcgtt taagcagaat caccagatca
     6841 cacttttgta catcaccgca ggtccgcagc cccatcgctt ccagctttgc cgctgagact
     6901 ttgccaacac cgggaatttt tgccagcggt aaggtttgca gaaatgccga aacttctgcc
     6961 ggcgtaatca caaactggcc gttgggttta ttcatgtcgg aggcgatttt ggcgagaaac
     7021 tttactggtg ccacgcccgc agacgccgtc agttgcagct cgctgaaaat tgtctggcgg
     7081 atttcctggg cgatgagggt cgcagaaccg tggcaatgga cgctatcagt gacatcgagg
     7141 taggcttcat ccagtgacaa cggttcaata cgcgaggtgt agcgcgagaa gatttcacgg
     7201 atatgatttg aggcttcttt gtaggcgtca aagcgccccg gaagcaaggt gagatgtggg
     7261 cataatttga gcgccatccc tgtcggcata gcgctgcgta cgccaaattt acgcgcggga
     7321 taattggcgg tgctgatcac cccccgacgt tcgcggctgc cgccaatagc aatagggata
     7381 tcgcgcaggg cgggattgtc gcgcatctcc actgcggcga aaaagcagtc catatccaca
     7441 tgaatgattt tacgcattac tcacctctca acactggtaa agtatacagt gatttcaggg
     7501 tttgagaaat gcgtaaagat tcagcattta gcgcgttcgc tgcgaaagca cctgctgctt
     7561 atccagcttt tgcaccagcg gctgcaccac tttatcccca tgctgaccaa agtattgata
     7621 cagcgtatcg gacgcacttt tggtcagcac cataatctca atgcgtcggt tgcccgcgct
     7681 ttgcggattt ttcgcgtcca gcagcatctg gtccgccatt gcgcttacct gcatcacttt
     7741 atcttccggc attccggcct cttccagcac ccgacgagct gaaagcgcgc gatcgcccga
     7801 aaggttccag ttgttgtaga tattgttttt gtaggccatc gcatcggtat gcccggtaat
     7861 aataattttg ttatcgagcg agtcgaacac tggcgcgagc tccaccagca aagttttaaa
     7921 gaacggcata atctgcgcgc tgccgcgttc gaacatattg cgattctggt cgtctttaat
     7981 cagcacgcgt aatccctgcg ggacaatctc catctccaga ttcgcttcca tatgcgcatc
     8041 acgggcaatg gtgttgatac tggttgccag ctcgccaagc tcggtggcgg atttcttctt
     8101 cagcagcgcc gttttctcat tcacatcacg cgcttttttc tccgtctctt ccggcaccgc
     8161 cacggtggca ggttttggcg gatgagacgg gctgatttta tttaacggcg acagcccacc
     8221 accgttaaaa atcgactgcc cgtgcagcgc ggcgataatg ctttcacgct cggatttact
     8281 gacgctgttg acaatcaact ttatcgtcgt caccaaaggg gccgagcgta tttccgaggt
     8341 ttctgcccgc tttaccctgg atgcgatgcc cggcaaacag atggcgattg acgccgatct
     8401 taacgccgga ttgatcaacc aggcacaggc gcaaacccgg cgtaaagatg ttgccagcga
     8461 ggccgatttc tacggcgcga tggacggggc atcgaagttt gtgcgcgggg atgccatcgc
     8521 cgggatgatg attctggcga ttaacctgat cggcggcgtc tgtatcggga tatttaaata
     8581 caacctgagc gccgacgccg ccttccagca atatgtgctg atgaccatcg gcgatgggct
     8641 ggtggcgcag atcccttccc tcctgctctc caccgcagcg gcgattatcg tcacccgcgt
     8701 cagcgacaac ggcgatatcg cccatgacgt gcgccaccag ctgctggcaa gcccgtcggt
     8761 gctctacacc gccaccggga ttatgtttgt gctggcggtg gtgccgggaa tgccgcacct
     8821 gccgtttttg ctgttcagcg ccctgctggg ttttaccggc tggcggatga gcaaacagcc
     8881 gcaggcggcg gaggcggaag agaaaagcct cgaaacgctg acccgcacca tcactgaaac
     8941 cagcgagcaa caggtcagtt gggaaaccat tccgcttatc gagcctatca gtttaagcct
     9001 cggctacaag ctggtggcgc tggtcgataa agcgcagggc aacccgctca cccagcgtat
     9061 tcgcggcgta cggcaggtga tatccgacgg taacggcgtg ctgctgccgg agatccgcat
     9121 tcgggaaaac ttccgcctca agcccagcca gtacgccatt ttcatcaacg gcattaaggc
     9181 cgatgaagcg gatattccgg cggataaact gatggcgctg ccctccagcg aaacctacgg
     9241 cgagattgac ggcgtgctgg ggaacgaccc ggcgtacggg atgccggtga cctggatcca
     9301 gcccgcgcag aaggcgaagg cgctgaatat ggggtatcag gtgatcgaca gcgccagcgt
     9361 gattgccacg catgtgaaca agattgtgcg cagctatatt cctgatttgt ttaattatga
     9421 tgacatcacg cagctgcata accgcctgtc gtcgatggcc ccgcgcctgg cggaagattt
     9481 gagcgcggcg ctgaattaca gccagttgct gaaagtgtac cgtgcgctgc tgaccgaagg
     9541 cgtttccctg cgcgatatcg tcaccatcgc caccgtgctg gtcgccagta gcgcggtgac
     9601 taaagatcat attctgctgg cggccgatgt gcgcctggcg ctgcggcgca gcattaccca
     9661 tccgttcgtt cgcaagcagg agctgacggt gtatacgctg aataatgagc tggaaaatct
     9721 gctgactaac gtagtgaatc aggcgcaaca gggcggtaaa gtgatgctcg acagcgtgcc
     9781 agtggacccg aatatgctca accagttcca gagcacgatg ccgcaggtga aagagcagat
     9841 gaaagcggcg gggaaagacc cggtgctgct ggtgccgccg cagctgcgcc ctttgctggc
     9901 gcgctatgca aggttgtttg cgccggggct gcatgtgctg tcgtataacg aagtgccgga
     9961 tgagctggag ttgaagataa tgggggcgtt gagttgattg gcctttaagg ggcgcatccg
    10021 gcattggtgc acaattgccg gatgcggcgt gaacgcctta tccggcctac ggttgagtgc
    10081 agttttgtag gcatgataag acgcgccagc gtcgcatcag gcatccggca ccagcgcaca
    10141 attatgaaat gatacgctcc ccggcactaa aatccgttat gtctcccgcc caatcaatgg
    10201 gatataaccc tcgcgccaca tcgcgatgga acgttgagaa tggccaatca ctcacttgct
    10261 ttacccagcc atgttttact ggatttatat aaatataatc aacatgatgg cgatagtctt
    10321 tggtattgcg gatggtgtgc tcctaaaaac gtggttgcca gatatttttc aatccacaag
    10381 catgggtgaa ctgcttttta atctcccgcc agcgggagga aaaatcatca tcgctttcgg
    10441 gtaatgtcca tatgcaatgc atatgctccg gcaaaacgac ccaggcgtta atttcaaaag
    10501 gtctgtctcg cttaacgtta ataatggcgt tacggagcat ctggaactga gcggtcagaa
    10561 ggtggcttcg acgatttcgt aggttaaccg tgaaaaacca ggttccccct ttgatgtaat
    10621 accgacgata ttcagacatg gcaactcctt tttctcaacg gtaagaagaa aaggaaaggt
    10681 cggcaatgag gtgatggaat aacgggaaag cggctcgcaa aaacgcacag atttgtaggc
    10741 atgataagac gcgtcagcgt cgtatcaggc atctgcgcac aactgccgga tgcggcgtga
    10801 atgccttatc cgtcctacaa aatcgtctct tccgtcaaaa tccggcgcgc gcctaaaaag
    10861 tgatcctgcc agaaaggttc agctaaccga cttacccgaa tggtttcccc ggtgcgcggt
    10921 gactcaataa actgaccgtc gcccagatac acccccatat ggtcggcaat ctcgcggctg
    10981 tggatatgga aaaacagcaa atctccccgg cgcaggtcgt tattcgccac aatcgttgcc
    11041 cgatggtagt gatacatctc attagccgta cgcgggagct tcgcctcgag gatcttgttg
    11101 taggcataaa aaaccaaccc gctacagtca aagcctttat caggccgcgt gccgccccag
    11161 acgtaaggct tgccaagctg ctgctccagg cggtgaatcg ccacttcggt aatgttatgc
    11221 agatggtcgc tgcttaaaaa gtggttgttt tccgccagcg cctgccagcg tctgtcactc
    11281 gccttgagcg gtgcaggaaa ccattctgga tgctgtttta tctgttcgcg gttgtgctgg
    11341 cgtagcgccc ttctgctttc ggcattgcct tccacaatat agctgacctg ctttttcaga
    11401 tgggtttggt actgttttaa taaacgcgca cggttttgca tccgctggcg ggcggcgtag
    11461 ctgaaagaga tatgtgatgt ggtggcgctg gcaaatgaag aaaacggcaa acaaatcaac
    11521 agtaatcccg acaatacaaa ggatggaatc gacggcaagg acatactcaa taatggctgg
    11581 tcatgcattg atgttacagt gtatcccggc ccgtacctga acaaacttaa taaaccatta
    11641 ttataaaatt aataaatata tttcagttaa ttatgtaaaa acatccgttt attaaattac
    11701 tcatagattg tcgctgttgc tcatttgagc tacaattcaa gctgaataaa tatacagcat
    11761 aggagatatc ccaatggctg ctaacgcatt tgttcgcgcc cgaatcgatg aagatctgaa
    11821 gaatcaggcg gcggacgtac tggccgggat ggggctgacc atctctgacc tggttcgcat
    11881 aaccctcaca aaggtcgcgc gtgaaaaggc attgccgttt gatttacgcg agcctaatca
    11941 attaaccatt caatcaatca aaaacagcga agctggcgtt gatgttcata aggccaaaga
    12001 cgccgatgat ttatttgata aattaggagt ttaaatatga ttcaaaggga tattgaactc
    12061 gggacaattt tcaaaggatg taaaacttgc acaaaagcgt cataaggata tgaataaatt
    12121 gaaatatctt atgacgcttc ttatcaataa tactttaccg cttccagctg tttataaaga
    12181 ccacccgctg caaggttcat ggaaaggtta tcgcgatgct catgtcgaac cggactggat
    12241 cctgatttac aaacttaccg ataaactttt acgatttgag agaactggaa ctcacgcggc
    12301 gctctttggg taaagcccgc tgccgatgaa atttgtaaac cctgcgccga caggcttgcg
    12361 taaaactctt ttggtgataa tgtgagcctt cccaatccag actattccga tttgggatcc
    12421 attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa taagcagtat gcgtaaaatc
    12481 gcattaattc ttgcgatgtt gttgatcccg tgcgtttcgt ttgccggttt gctgggcagc
    12541 agtagctcga ccacgccagt gagcaaagag tataagcagc agttgatggg atcccctgtc
    12601 tacatccaga tcttcaagga agaacgtacg ctcgatctct acgtcaaaat gggcgagcaa
    12661 tatcaactgc tcgacagcta taaaatctgt aaatattccg gcggcttagg gccaaaacag
    12721 cgtcagggcg atttcaaaag cccggaaggg ttttatagcg tccagcgtaa tcagttaaaa
    12781 ccagacagcc gttactacaa agcgattaat attggtttcc ccaatgccta tgaccgtgcg
    12841 catggttatg aagggaaata cctgatgatc cacggcgatt gtgtttccat cggctgctac
    12901 gcgatgacca atcagggcat tgacgagatt ttccagttcg ttactggcgc actggtattt
    12961 ggtcagccga gcgtgcaagt gagcatttac ccgttccgca tgaccgacgc caatatgaag
    13021 cgccataaat attccaactt taaggacttc tgggagcaac tgaagccagg ctacgactac
    13081 tttgagcaaa cccgtaagcc accgaccgtt tctgtggtca atggccgtta cgtggtcagc
    13141 aagccgttaa gccacgaagt tgtgcaacca caactggcat caaactacac gctccccgag
    13201 gcaaaataag cgccattcgc ctggcataat cttttgccag gtttcattgc ccgtcagcgg
    13261 ctgtgtcgca atcaccgtga ccacatcatt cggtgtggtc tgcgagctga agtcgatttc
    13321 cacatcctga tccagcaacg ttgccacgcc aaacggcgcg cggcgggtga tccagtgcag
    13381 attggtcgag caatacgcca ttacatagcg cccatccgaa agcagcatgt tgaaaacgcc
    13441 cttctgccgc agttcatccg ccagtgaggc gatatattta aataccgccg ccatattgcc
    13501 cggcgtacgc ggataacgct gcgttaattt atacagcagc cagcaaaagg ctttttcgct
    13561 gtcggtttta cctaccgggc ggaagttgcc ggtttccagt gatttatagc ccgtcagttg
    13621 tccgttatgg gcataagtcc agttacgtcc ccataactcg cgggtaaacg ggtgagtatt
    13681 ttccaacgcc acctcgcccc gatttgcctg gcgaatatga gccacaaccg aacaggattt
    13741 tatcgggtag tcctgaacaa gtttggcaat gggggaatta aagctgggtt gtggatcttt
    13801 aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc cagccatctt tatgtggccc
    13861 ggttccacca ccacgctgca ccagcccggt gaaactaaag caaatatcgg tagggacgtt
    13921 ggcgctcatc ccgagcaatt cgcacatcgg taacccccac aacagcggga gcgcatggcc
    13981 cccggcgaag attgacttaa gaattttcag gccggatgcg gcgtaaacgc attattcggc
    14041 ctacgccaga cttacttaac catctctttt tcaattaact ggatcaggat atggatcact
    14101 ttaatgtgaa tttcctgaat gcggtcggca taaccaaaat gtggaacgcg aatttcaata
    14161 tccgccgtac cagccatttt gccgccatct ttgccggtca gggtaatcac tttcattccc
    14221 ttctcacgcg ccgccgcgat cgctttgatc acgtttgcag agttaccaga cgtggagatc
    14281 cccagcagta catcgccttc gcggcctacc gcttcaacat agcgggagaa aatatcattg
    14341 aaaccaaaat cgttaccgac gcaggaaata tggctgacgt cagagatggc aatcgccggg
    14401 tagcccggac ggttttcacg gtagcgaccg gtcagctctt cggcaaagtg catggcgtcg
    14461 caatgggagc cgccgttgcc gcaggaaagc accttgccac cggctttaaa gctgtctgct
    14521 aacaggaccg ccgctcgctg aatggcgtga atattggcgt catcttttaa aaagttagcc
    14581 agcgtttccg ccgcttcgtt cagttcgtta cgaataagat cctggtacat gaggatatcc
    14641 ttcagcataa atgtaataga caaaatgcag tgtaccggat accgccaaaa gcgagaagta
    14701 cgggcaggtg ctatgaccag gactttttgc cctgaagtgt agataaaaac agcaacaatg
    14761 tgagcttcgt tgtaattata ttgtaaacac attgctaaat gtttttacat ccactacaac
    14821 catatcatca caagtggtca gacctcctac aagtaagggg cttttcgtta tgatgatttt
    14881 gagtattctc gctacagttg tcctgctcgg cgcgttgttc tatcaccgcg tgagcttatt
    14941 tatcagcagt ctgattttgc tcgcctggac agctgccctc ggcgtcgctg gtctgtggtc
    15001 ggcgtgggta ctggtgcctc tggccattat cctcgtgccg tttaactttg cacctatgcg
    15061 taagtcgatg atttccgcgc cggtatttcg cggtttccgt aaggtgatgc cgccgatgtc
    15121 gcgcactgag aaagaagcga ttgatgcggg taccacctgg tgggaaggcg acttgttcca
    15181 gggcaagccg gactggaaaa agctgcataa ctatccgcag ccgcgcctga ccgcagaaga
    15241 gcaagcgttt ctcgacggcc cggtagaaga agcctgccgg atggcgaatg atttccagat
    15301 cacccatgaa ctggcggatc tgccgccgga gttgtgggcg tatctgaaag agcatcgctt
    15361 cttcgcgatg atcatcaaaa aagagtacgg cggcctggag ttttcagctt atgcccagtc
    15421 tcgtgtgctg caaaaactct ccggcgtcag cgggatcctg gcgattaccg tcggcgtgcc
    15481 gaactcatta ggccccggcg agctgctgca acattacggc actgacgagc agaaagatca
    15541 ctatctgccg cgtctggcgc gtggtcagga gatcccttgc tttgcactga ccagcccgga
    15601 agcgggttcc gatgcgggcg cgattccgga caccgggatt gtctgcatgg gcgaatggca
    15661 gggccagcag gtgctgggga tgcgtctgac ctggaacaaa cgctacatta cgctggcacc
    15721 gattgcgact gtgcttgggc tggcgtttaa actctccgac ccggagaaat tactcggcgg
    15781 tgcagaagat ttaggtatta cctgtgcgct gatcccgacc accacgccgg gcgtggaaat
    15841 tggtcgtcgc cacttcccgc tgaatgtgcc gttccagaac ggaccgacgc tcggtaaaga
    15901 tgtcttcgta ccgatcgact acattatcgg cgggccgaaa atggccgggc aaggctggcg
    15961 gatgctggtg gagtgcctct cggtaggccg cggcatcacc ctgccttcca actcaaccgg
    16021 cggcgtgaaa tcggtagcgc tggcaacggg cgcgtatgct cacattcgcc gtcagttcaa
    16081 aatctctatc ggtaagatgg aagggattga agagccgctg gcgcgtattg ccgggaacgc
    16141 ttatgtaatg gacgctgcgg catcgctgat tacctacggc attatgctcg gcgaaaaacc
    16201 tgccgtgctg tcggctatcg ttaagtatca ctgtacccac cgcgggcagc agtcgattat
    16261 tgatgcgatg gatattaccg gcggtaaagg cattatgctc gggcaaagca acttcctggc
    16321 gcgtgcttac cagggcgcac cgattgccat caccgttgaa ggggctaaca ttctgacccg
    16381 cagcatgatg atcttcggac aaggagcgat tcgttgccat ccgtacgtgc tggaagagat
    16441 ggaagcggcg aagaacaatg acgtcaacgt gttcgataaa ctgttgttca aacatatcgg
    16501 tcacgtcggt agcaacaaag ttcgcagctt ctggctgggc ctgacgcgcg gtttaaccag
    16561 cagcacgcca accggcgatg ccactaaacg ctactatcag cacctgaacc gcctgagcgc
    16621 caacctcgcc ctgctttctg atgtctcgat ggcagtgctg ggcggcagcc tgaaacgtcg
    16681 tgagcgtatc tcggcacgtc tgggtgatat tttaagccag ctttacctcg cctctgccgt
    16741 tctgaagcgt tatgacgacg aaggccgtaa tgaagccgat ctgccgctgg tgcactgggg
    16801 cgtacaggat gcgctgtatc aggcagaaca ggcgatggac gatttactgc aaaacttccc
    16861 gaaccgcgtg gttgccgggc tgctgaatgt ggtgatcttc ccgaccggac gtcattatct
    16921 ggcaccgtct gacaagctgg atcataaagt ggcgaagatt ttacaagtgc cgaacgccac
    16981 tcgctcacgt attggtcgcg gtcagtacct gacgccaagc gagcataacc cggttggttt
    17041 actggaagag gcgttggtgg atgtgattgc cgccgacccg attcatcagc ggatctgtaa
    17101 agagctcggt aaaaatctgc catttacccg tctggatgaa ctggcgcaca acgcgctggc
    17161 gaaggggctg attgataaag atgaagccgc tattctggtg aaagcggaag aaagccgtct
    17221 gcgcagtatt aacgttgatg actttgatcc ggaagagctg gcgacgaagc cggtaaagtt
    17281 gccggagaaa gtgcggaaag ttgaagccgc gtaattgaat aacggagccg aaaggctccg
    17341 tttctttatc cgctaattat ttaaaattaa agccatccgg atggttttcc aggctgccgg
    17401 tcaacgccgc gaacagcacc gttttaccat caatcgaaag cgcatcgttc acattcaacc
    17461 aggtcagttt ctcttgcgac gttttctcat caatagtcga gaacagcccc gtcatctgat
    17521 tagatttctc ggaccacatc acagcgatac gttgcgagcc acagtcatgc ggtttgcacg
    17581 cgctcatcac ctgatacgtc tcatctccca acgttacggt ttgtgcaggg gtataagtac
    17641 cgcctttcat cacccaggca ggcagcttat gcccttgcac catctgatta aatgcagctt
    17701 tggtggtttc gccctttgca aggctgctaa tggttaaatc atcctgcgcc attgcactgg
    17761 tggcgatgac cagtgcggcg actgtcgtta ttgccttaaa catcattcct cccgagctta
    17821 tcctgcccat gttattaacc tcctaaagcc tggcgataac acgataattt gcaaatcaga
    17881 tatttccaat atcagcattt gttatcaatt tattctgcgc taaggcaact gcctgataag
    17941 gtgttaaagt tataaaaccc ttcgcataaa ggagttgtac accgtgcctg gtttgaagat
    18001 tacgcttttg cagcaaccac tggtgtggat ggatggtcct gccaacctgc gtcattttga
    18061 tcgtcaactg gaaggtatta ccgggcgcga tgtgatcgtt ctaccggaga tgtttaccag
    18121 cggctttgcc atggaagcgg ctgcttcgtc gctagcacaa gatgacgtag tgaactggat
    18181 gacagccaaa gcgcagcagt gcaatgcgct gattgcaggc agtgttgcat tacaaacgga
    18241 gtctggttcg gttaaccgct ttttgctggt tgagccgggc ggcacggtac atttttatga
    18301 taagcgtcat ctgttccgca tggcagatga gcatctgcat tataaagcgg gcaatgcgcg
    18361 agtgattgtg gaatggcgcg gctggcgtat tttgccgttg gtgtgctacg acttacgttt
    18421 tccggtgtgg tcgcgcaatc tcaacgatta tgacctcgcc atctacgttg ccaactggcc
    18481 tgctccgcgc tctctgcact ggcaggcatt gctgacggcc cgcgcgattg agaatcaggc
    18541 gtatgtggcg ggatgcaatc gcgtcggcag cgatggcaac ggctgccatt atcgcggtga
    18601 cagccgggtg attaatccac aaggcgagat tatcgctact gccgacgcgc atcaggcaac
    18661 gcgcattgat gcggagctgt cgatggtggc attgcgggaa tatagagaaa agtttccggc
    18721 atggcgggat gcggatgagt ttaggttgcg gtgagtgggg gctgtattta gggcggggaa
    18781 ttcagaaagg gatgtttagt gtgctgagcg agagttaaat aataaaaaac tgggaagatt
    18841 taataatccc cccgcagggc aagagcatga attaaatctg tgttaaattt ttcattttgt
    18901 cagttcgaaa cccaagctct gagcaggatg caagcacttc ttctatataa catgtgttta
    18961 acgcaacctt ctttcttttc ctcttcaccc cacccttaat gtctttgcaa tctcttaata
    19021 aattcagtgc catcttcttt attccagata ttatttcggc tgcgtttcct cttcttatcc
    19081 ggctggcatc ttcattcatt tttacatcta acacccaatg aagactgtgc tcgatcagcc
    19141 agtgcgctct gatagcatgt gcaaattctt tagcatccat atcctttgat gaaatataat
    19201 atcggatgct tacaccttct gctgatttat cttctttctt ctgcctgaat gacaatgcta
    19261 cacaaagctt ttttaatccc ttccattcga attcaaaatc acaaaattca ggcgttacgt
    19321 tactgacaat atgcaaacgt gtttcttttc ttccatgact tatctcctgc gtactaaacg
    19381 aatcgccttt ataattagaa aacacattta caggaaattt ttcctcgaat gcatgatgta
    19441 atttcccctg attgcctttt actgccagaa gataatctgc ttttttatct ttgatcttcg
    19501 aagcgatatc tttctgacag cccatagcat caatggttat caaatttttc tttaaatcca
    19561 gtaggttaag caactctgga atggctgtaa tctcattact tttggcttcc gtttttacct
    19621 gccccagtac aacaccattt tcgttcgaga atgcactcac catatggatt gctccttttc
    19681 tttttccctt atcaaaggag cctcttatgg tctttccatc tattgctata atttcaccat
    19741 cagtgatttc atggcactcc tgcatccatt caataaatat cttttcaaag gccaaactgt
    19801 caatgttact cacaacgcgt gcaatggtgt catcgaccgg aatgccatta tcaaaatcac
    19861 catatttctt tagccattca agtctttcat gtccaaaatc ttcaatttcc tgccactcat
    19921 cggcacctgc aattactgca catacggtga gaaacaaaat atcagataat ttatgtttaa
    19981 cctttccttg ttgtcgtata tcagggatca ctgaaatata atcaagcaaa ctttgaatac
    20041 tcacctggcg tcctttttat ctttttagtt attccaaagg ttaaattttg atcacctgta
    20101 ggcgttaaag ccaggatgaa aatcgtgatt tacatcacga ttttcatgat cttgccctgc
    20161 cccgtccctt gctctcatgt ctttct
//
LOCUS       DAZFDC010000085        19998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFDC010000085 DAZFDC010000000
VERSION     DAZFDC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.085"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..581
                     /locus_tag="ACHJ48_003180"
     CDS             63..581
                     /locus_tag="ACHJ48_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO4708676.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            597..1136
                     /locus_tag="ACHJ48_003181"
     CDS             597..1136
                     /locus_tag="ACHJ48_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO4708677.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1229..2806)
                     /gene="guaA"
                     /locus_tag="ACHJ48_003182"
     CDS             complement(1229..2806)
                     /gene="guaA"
                     /locus_tag="ACHJ48_003182"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO4708678.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2875..4341)
                     /gene="guaB"
                     /locus_tag="ACHJ48_003183"
     CDS             complement(2875..4341)
                     /gene="guaB"
                     /locus_tag="ACHJ48_003183"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO4708679.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4503..5873
                     /gene="xseA"
                     /locus_tag="ACHJ48_003184"
     CDS             4503..5873
                     /gene="xseA"
                     /locus_tag="ACHJ48_003184"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO4708680.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQCNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5870..6085)
                     /locus_tag="ACHJ48_003185"
     CDS             complement(5870..6085)
                     /locus_tag="ACHJ48_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO4708681.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6154..7626)
                     /gene="der"
                     /locus_tag="ACHJ48_003186"
     CDS             complement(6154..7626)
                     /gene="der"
                     /locus_tag="ACHJ48_003186"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO4708682.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7744..8922)
                     /gene="bamB"
                     /locus_tag="ACHJ48_003187"
     CDS             complement(7744..8922)
                     /gene="bamB"
                     /locus_tag="ACHJ48_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO4708683.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPAVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8933..9553)
                     /gene="yfgM"
                     /locus_tag="ACHJ48_003188"
     CDS             complement(8933..9553)
                     /gene="yfgM"
                     /locus_tag="ACHJ48_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO4708684.1"
                     /translation="MEIYENENDQVEAIKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9571..10845)
                     /gene="hisS"
                     /locus_tag="ACHJ48_003189"
     CDS             complement(9571..10845)
                     /gene="hisS"
                     /locus_tag="ACHJ48_003189"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO4708685.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10956..12074)
                     /gene="ispG"
                     /locus_tag="ACHJ48_003190"
                     /gene_synonym="gcpE"
     CDS             complement(10956..12074)
                     /gene="ispG"
                     /locus_tag="ACHJ48_003190"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO4708686.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12101..13114)
                     /gene="rodZ"
                     /locus_tag="ACHJ48_003191"
     CDS             complement(12101..13114)
                     /gene="rodZ"
                     /locus_tag="ACHJ48_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO4708687.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPVVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13399..14553)
                     /locus_tag="ACHJ48_003192"
     CDS             complement(13399..14553)
                     /locus_tag="ACHJ48_003192"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO4708688.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14703..15134)
                     /gene="ndk"
                     /locus_tag="ACHJ48_003193"
     CDS             complement(14703..15134)
                     /gene="ndk"
                     /locus_tag="ACHJ48_003193"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO4708689.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15275..16129)
                     /locus_tag="ACHJ48_003194"
     CDS             complement(15275..16129)
                     /locus_tag="ACHJ48_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO4708690.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIDCAVCQFICPQEAIRGVNSPLRYFCNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNECLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16129..16950)
                     /locus_tag="ACHJ48_003195"
     CDS             complement(16129..16950)
                     /locus_tag="ACHJ48_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFO4708691.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLMLAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16943..17572)
                     /locus_tag="ACHJ48_003196"
     CDS             complement(16943..17572)
                     /locus_tag="ACHJ48_003196"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFO4708692.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17569..19950)
                     /locus_tag="ACHJ48_003197"
     CDS             complement(17569..19950)
                     /locus_tag="ACHJ48_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFO4708693.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYYFQKMKQNGTRFIVVDPRYSDTVSSLADQWIP
                     LLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHICGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADVLLPETSFMERWNIGETWGTASYLILSEKPIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDRGVSIIPVEITPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
BASE COUNT         4951 a         5419 c         4856 g         4772 t
ORIGIN      
        1 ttactttcct cttttgatga tcttaaatgt cttatttttc gtaatgtgta taacaaggaa
       61 tagtgatgaa atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca ttcactctgg
      121 caggatgcca gtcaaatgct gacgatcatg ccgccgatgt ttatcaaacc gatcaactga
      181 ataccaaaca agaaactaaa accgttaata ttatttccat tcttcccgca aaagttgccg
      241 tagacaactc ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt ggcgcagtcg
      301 ctggcggtgt tatcggccac aacgtcgggt ctggcagcaa ttccggaacg acggcaggtg
      361 cagttggcgg cggagctgta ggcgcggcag cgggttctat ggtgaatgat aaaaccttag
      421 tggaaggtgt ttctttaacg tataaggaag gcaccaaagt gtatacctct actcaggtgg
      481 gtaaagagtg ccagtttacg acaggtttag ccgttgttat taccacgacg tataacgaaa
      541 cgcgtattca gccaaatacc aaatgtcctg aaaagagcta ataatcagga ggagtcatga
      601 agaaagtttt tctttgcgcc atcttagcct ccttaagcta tccggctatc gcctcatcat
      661 tgcaggatca actctctgct gtcgcagaag cggaacagca aggtaaaaat gaagagcaaa
      721 ggcagcatga cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc
      781 gcgcaaatgc ccaggctgcc gctaacaaaa gagcggcaac ggcagcggca aataagaaag
      841 ctcgtcagga taaactggac gccgaagcca ctgcggacaa aaaacgcgat caaagttatg
      901 aagatgagct acgcagctta gagattcaga aacaaaaact ggcgctggca aaagaagaag
      961 cccgcgttaa gcgagaaaac gaatttatcg atcaggagct gaagcacaaa gccgcgcaaa
     1021 ctgatgtcgt gcaatctgaa gctgacgcca acagaaatat gactgaaggc ggtcgcgatc
     1081 tgatgaaaag cgtgggcaaa gcagaagaga ataaatcgga cagctggttt aattaatcga
     1141 tgttagtaac ttcaagccta taattcttga agataaaaaa ccctctgtag taacagaggg
     1201 ttttgttcat tcatagtgca gggtcaaatc attcccactc aatggtagct ggtggcttgc
     1261 cgctgatgtc atacaccacg cgggaaatac cgttcacttc attgataatg cggttggaaa
     1321 cgcgaccgag gaaatcgtac ggcagatgcg cccagtgtgc ggtcataaag tcgatggttt
     1381 cgacagcacg cagagagaca acccagtcat acttacgacc atcgcccatt acgccaacgg
     1441 aacgtactgg caggaagacg gtgaacgcct ggctgacttt gtcgtacagg tccgctttac
     1501 gcagttcttc aatgaagatg gcgtcagcac ggcgcagcag gtcacagtac tctttcttca
     1561 cttcacccag aacacgaacg ccaaggcctg gtcccgggaa cgggtgacgg tacagcatgt
     1621 cgtacggcag gcccagctcc agaccaatct tacgcacttc gtctttgaac agctctttca
     1681 gcggttcaac caggcccatc ttcatctctt tcggcaggcc gcccacgttg tggtgagatt
     1741 tgatgacgtg tgctttaccg gttgcagacg ccgcagattc gataacgtca gggtagatgg
     1801 tgccctgcgc cagccacttc acgtcttcca gtttcagcgc ttcttcatcg aatacttcaa
     1861 cgaaaacgcg accgatgatt ttacgttttg cttccggatc gttttcgcca gccagcgctg
     1921 acaggaagcg atcttctgcc ggtacgtgaa caatgttaag accaaagtga tcgccaaaca
     1981 tatccagaac ctgctctgct tcgttgagac gcagcaggcc gttgtcgacg aatacgcaag
     2041 tcaggttttt accgatagcg cggtgcagca gcattgcggt tacggaggaa tccacaccac
     2101 cagagaggcc gaggatgact ttatcgtcgc ctacctgctc gcggatgcga gctacagcat
     2161 cgtcgataat tttcgctggc gtccacaggg cttcacactg gcagatatca cgcacaaaac
     2221 gctccagcat gcgcataccc tggcgggtgt gagtcacttc cgggtggaac tgtacgccat
     2281 agaagcgttt ttcttcgtta gccataatgg caaacgggca gctttcggtg ctggctacgg
     2341 tgacgaagtc ggacgggata gcggtaactt tatcgccgtg gctcatccag acatcgagca
     2401 gcggtttacc gtctgcggtc agcgcatctt cgataccgcg aaccagtgcg ctgtcgttta
     2461 cgacttcaac ctgcgcgtag ccaaattcac gttcgttaga ggcttcaacg tgaccgccca
     2521 actgcattgc catggtctgc atgccatagc aaacgccgaa taccggtacg cctgcttcaa
     2581 agacatactg cggcgcacgc gggctgtttt cttcagtagt actttccggg ccgccggaaa
     2641 gaataatgcc gcttggattg aagtcacgaa tttgtgcttc tgtcacatcc catgcccaca
     2701 gttcgcagta aacacccagc tcacgcacgc ggcgcgcaac cagttgagtg tactgagaac
     2761 cgaagtccag aatgaggatg cgatgcttat gaatgttttc cgtcattgac gcttattccg
     2821 aggcaagtga aacagataat ataaatcgcc cgacatgaag tcgggcgaag agaatcagga
     2881 gcccagacgg tagttcgggg actctttagt aatggtcacg tcgtgaacgt ggctttcctg
     2941 aatgcccgca ccgctgatac gtacaaactc cgctttagta cgcagttcgt cgatagtacc
     3001 acagccggtc aaacccatac aggagcgcag gccgcccatc tgctggtgaa tgatctcttt
     3061 caggcgacct ttataagcta cgcgaccttc gataccttcc ggcaccagtt tgtcggcagc
     3121 gttatcgctc tggaaataac ggtcagagga acctttggac atcgcgccca gggaacccat
     3181 accacggtaa gatttgtaag aacggccctg gtagagttcg atttcacccg gagattcttc
     3241 agtacccgcc agcatggaac ctaccatcac cgcgcttgcg ccagcggcga tagctttggc
     3301 gatgtcgccg gagaagcgaa taccaccatc tgcgataacc ggaataccgg tgccttccag
     3361 ggcttcaact gcgtcagcaa cggcagtgat ctgcggaaca cctacgccag taacgatacg
     3421 agtcgtacag atagaaccag ggccgatacc gactttaacc gcgctgcaac cagcttctgc
     3481 cagagcgcgt gcacctgcag ctgttgccac gttgccgccg atgatttgca gatccggata
     3541 tttagcacgg gtttcacgga tacgctgcaa aacgccttct gagtgaccgt gggaggagtc
     3601 gatcagcaga acgtcaacgc cagcggcaac cagcgcgtca acacgctctt cgttacccgc
     3661 acctgcgcca actgcagcac caacacgcag acggccttgc tcgtctttac aggcgttcgg
     3721 tttacgttcc gctttctgga aatctttcac ggtgatcatg ccgatcaggt ggaattcgtc
     3781 atcaaccacc agcgcttttt caacgcgttt ttcgtgcatt tttgccagca ccacttcacg
     3841 ggcttcacct tcacgcacgg tgaccagacg ctctttcggc gtcatgtaaa cgctaaccgg
     3901 ctggttcagg tcggtaacaa aacgcacgtc acgaccggtg ataatcccca ccagttcgtt
     3961 ttcttcggta acgaccggat agcccgcaaa accgttacgc tcggtcagtt ctttcacttc
     4021 gcgcagcgtc gtggttggca gaacagtctg cggatcagtc accacaccag attcgtgttt
     4081 tttcacacgg cgaacttctt ctgcctggcg ttcaatggac atgtttttgt ggataaagcc
     4141 gataccgcct tcctgagcca gagcaatagc caggcgcgct tccgttacgg tatccattgc
     4201 tgcggaaagc atagggatat tcagacgaat agttttcgtc agctgggtgc tgaggtcagc
     4261 agtattcggc agaacggtag aatgagcagg aacgaggaga acgtcgtcaa acgtcagagc
     4321 ttctttagcg atacgtagca tgggcaatat ctcgaccaga gtggttaata aatattgccg
     4381 cggcattata cagagcgtaa ccgattgcat ctaccccttt ttgcaaaaaa tgcttgctat
     4441 ccccgaaggg cgggttacta tcgactgaat aacctgctga tttagaattt gatctcgctc
     4501 acatgttacc ttctcaatcc cctgcaattt ttaccgttag tcgcctgaat caaacggttc
     4561 gtctgctgct tgagcatgag atgggacagg tttggatcag cggcgaaatc tctaatttca
     4621 cacaaccggc ttccggtcac tggtacttta cactcaaaga cgacaccgcc caggtacgct
     4681 gcgcgatgtt ccgcaacagc aaccgccggg tgaccttccg cccacagcat gggcaacaag
     4741 ttttagttcg cgccaatatt acgctctacg agccgcgcgg cgactaccag ataatcgttg
     4801 agagtatgca gccggccggt gaagggctgc tgcaacagaa gtacgaacag ctcaaagcga
     4861 agttgcaggc tgaaggattg ttcgatctgc aatacaaaaa accacttccc tcccctgcgc
     4921 attgcgttgg tgtgatcacc tcaaaaaccg gtgctgcgct acatgatatt ttgcatgtgt
     4981 taaaacgtcg cgatccttct ctgccggtga tcatctaccc caccgccgtt cagggcgatg
     5041 acgcgccggg gcaaattgtt cgcgccattg aactggcgaa tcagtgcaat gagtgcgacg
     5101 tgttgatcgt tgggcgcggc ggcggttcgc tggaagattt atggagtttt aacgacgaac
     5161 gcgtagcgcg ggcgattttt gccagccgca ttccggtcgt cagcgccgtc gggcatgaga
     5221 cggatgtgac cattgccgat tttgttgccg atctgcgtgc accaacaccg tcggctgccg
     5281 ccgaagtggt gagccgtaat cagcaagagt tactgcgcca ggtgcaatcg acccgtcaac
     5341 ggctggagat ggcgatggat tattatctcg ccaaccgcac acgtcgcttt acgcaaattc
     5401 atcaccgatt acagcaacag catccgcagc tccggctggc acgccagcaa accatgcttg
     5461 aacgcctgca aaaacggatg agctttgcgc tggaaaatca acttaagcgt gccgggcaac
     5521 agcagcagcg attaacacag cggctgaatc agcaaaatcc acagccgaag attcatcgcg
     5581 cgcaaacgcg cattcagcaa ctggaatatc gtttagcaga aaccctgcgc gcacagctta
     5641 gcgccacgcg tgaacgtttc ggtaatgcag taacgcacct cgaagccgta agcccactgt
     5701 caacgctggc gcgtggatac agcgttacta ctgctactga cggcaaggta ctgaaaaaag
     5761 tgaagcaggt taaagcgggt gaaatgctaa ccacacgtct ggaagacggc tgggtagaaa
     5821 gtgaagtaaa aaacatccag ccggtaaaaa aatcgcgtaa aaaagtgcat taagccagca
     5881 cgaattcaac gcgttttttc gatatcaagc catgcccgtg ctgacagaag taatctaccg
     5941 caccgcaggc ttttaacacc tgcaacggtt gatggcagtc cgggcaaaga gctttcattt
     6001 caataacttt cccgcaggat gggcaatacg catggccgtt atcctgatca agcacatgct
     6061 gacattgtgg acaatgcagt tccatatgaa ttcctccggt agccacccct ctacattcat
     6121 agagggaatg gcagataaaa tacttacgga taattattta tttttcttga tgtgcttcat
     6181 cagacgctta cgtttacgca tctgggttgg cgtcagggtg ttacgcttgt tcgcatacgg
     6241 gttttcccct tctttgaact gaatacgaat cggcgatccc attacgtcca gcgatttgcg
     6301 gaagtagttc atcaagtaac gcttgtagga atcaggcagg tctttcacct gattaccgtg
     6361 aatcaccaca atcggcggat tataaccacc ggcgtgtgca tatttcagct tcacacgacg
     6421 accgcgtacc agcggcggtt ggtgatcttc aacagccatc gtcatgatgc gcgtcagcat
     6481 agaggtcccc acacgacggg tggagctgtc atacgcttca cgtactgatt caaacaagtt
     6541 accaacacca ctgccgtgca aggcagagat aaagtgcaca cgagcaaaat cgataaagcc
     6601 cagacggaag tccagcgttt ctttcacctg ctctttcact tcctgactca ggccatccca
     6661 cttattcacc acaatgacaa gtgagcgccc actattgaga ataaagccca gcagcgagag
     6721 atcctgatcg gaaatacctt cgcgcgcatc aatcactaac atcaccacgt tggcgtcttc
     6781 aatggcctgc aacgttttga ttacggagaa tttctctaca gcatcggtga ttttgccgcg
     6841 tttacgtacg ccagcggtgt caatgagcac atactcacgt ccatcgcgtt ccattggaat
     6901 gtagatactg tcacgcgtcg taccaggcat gtcataaaca acaacgcgct cttcaccaag
     6961 aatacggtta gtgagtgtag acttacctac gttcggacga cccacaatcg ccagtttgat
     7021 cggcagactt tgcgggtcga agtcgtcttc ctcttcttct tcgccgttct cttccgcttc
     7081 aaattgcgcc cagtattcag cgtcttcgtc gacttcctct tgcggtgcga gatcttccat
     7141 ccacggcagt agcacatgct ccagcagact taatacgcca cgaccgtgag acgcggcgat
     7201 cgggtagatt tcacctaaac caagcgagta gaaatcaacc actgcctgat cgggatccag
     7261 accgtcagtt ttgtttgcca ccaggaaggt cggtttttca cgggagcgca gatgtttggc
     7321 aatcgcttca tctgccggca tcaggcccgc gcgcgcatcc accataaaca gtacgacgtc
     7381 cgcttcttca atcgccagca gcgactgttc cgccatgcgg gtttctacac cgtcttctgt
     7441 gccatcaatc ccgccggtat caatacagat aaactcacgg ccttcaattt ccgcacgacc
     7501 gtacttacgg tcacgagtca gacccgggaa atccgcaacc agcgcatctc gggtgcgagt
     7561 tagacggtta aataacgtgg attttcctac gttagggcgc ccgacaagcg cgaccacagg
     7621 taccatgttt aaagcctcat ttttataaat catcagacaa cgcacgctat attcgcgtcg
     7681 ttgttaaaaa caggaaaacg gcccctgtcc aggagccgtt ttcaaagtga acgacagaga
     7741 cgattaacgt gtaatagagt acacggttcc gtcttttgcc tggatcagca gtttgccgtc
     7801 agcggcaacc ggttcagtct ggaaaccgga actatcaact ttttgctggg caacgaaacg
     7861 accatcttcg acgttaatcc agtgcaaata accttcactg tcaccgacca ccaggttgcc
     7921 attatacagc accggagaag tcagcaggcg atgcagcaga tcgctttgtg tccacagcgt
     7981 aacgccgcca tcaatggtca acgccatcac ccggtcattt tgatcgacca gatagatgcg
     8041 attgccgtcg acgatgaaat cattcaccga acccagttcg cgtttccaca taatctgacc
     8101 actgcgcaga tcaagcgccg tcaggttacc attataggcc agcgcgaaaa caacgccgtt
     8161 aacaacggcg ggagtcgtgt caacatcgct cagacggtca atttcggtag aaccggtcgc
     8221 ttgggaaata cgctgctgcc aaatcatctg gccctgttcc atcagcactg cgctgacgcg
     8281 accattatcg ccccccacga cggccgcacc aaaagccgtt gccggcgcag actcgccacg
     8341 caaagagagc gaaggcatat cgaggttaac tgtccatttg acagcgccgt cagcttcgtt
     8401 cagcgcttgt aactgaccgt tactggtgtg gattaacacc agaccgtcgc tgaccaccgg
     8461 gcgcgaaagt gcttcacccg cgactttagt ttgccatgcc acagtaccat cgctggtatt
     8521 cagcgcgtaa acctgcgcct tttcgctgcc aatgtagaca tgtccaccag acacggtcac
     8581 accgccagaa agtaatgcag gctctttaga gaaccagcca tctttctcgg ccaggctgac
     8641 agaccagatt tctttgccat catccgcatt cagcgctttt actaaaccag cgcggtccgc
     8701 tgcatagaca acgttgtccg ccagtgccgg atgaagattg gaatagaagt tgccaatgcc
     8761 gctaccaacg gaagtgctcc acgccgtggt cggcgtaaac tggttttcaa cggttggcaa
     8821 tggggacatc tttaccacat cttcttcgct gttaaacagc gaacagccgc ttaaaagggt
     8881 aacggaaagc agtcctggca gcagtaattt acgcaattgc atcgggtccc tctcagatgg
     8941 acaaattatt aattttcatc tgcatcattt cgctcagtgc cggagtaaca tcgcttttca
     9001 cgcctgcttc ccatgcacta cgcgcacctt gcttatcacc tttgctcagc aatgcttcac
     9061 cacgcaggtc ggcaacaatg gcagcccacc cttcaccttt gatggtatca agggttttca
     9121 gcgcggcatc agcctgcttg agctgtacct gaacgcgagc aagacgcaga tttatcacgg
     9181 ctttgagatt ttcatcgctc gtgtctgcca gcccctgttg taactgggcg gcagctttct
     9241 ctagttcatt tttgtcaaca aattgctgcg caagttccaa agaagccagc gcaccataag
     9301 tatttttatt ttcagcagca aatttttccg ccgccgggat gctatccggt ttgccttcgc
     9361 tcactgcggt aaccgcattt tgataggcaa gagaagcgga gcgtgcagaa tcaacctgat
     9421 ggctgttcca gtagcgccag ccaatcagtg cgccaacgcc caaaatcacc ccaacagcca
     9481 gtgctttgcc attttcagca aaaaagcgtt taatcgcttc tacctggtcg ttttcgttct
     9541 cgtaaatttc cacgctgtcc ttctccttcc ttaacccagt aacgtgcgca aatgcgcggc
     9601 tacgctatcc tgcgcaactg ccgtttgctc accagagcgc aaatccttca ctactgctgt
     9661 gccgttagcc acttcagact cacccagcac cacagcaacg cgggcacccc atttatcagc
     9721 acgggcaaac tgtttcttaa agttgccgcc gccgtggttg gtcatcaatt tcacgcccgg
     9781 taattcatca cgcagacgct cagctaatgc catagccgca gattgtgtat cagcacctga
     9841 agccaccagg tatatatcga caacaggatc ggctttaaat tccggattaa cggcctgtac
     9901 taacaataca agacgttcga ggcccatcgc aaaaccgaca gccggtgttg cacggccgcc
     9961 cagttgttcc acaagaccgt cataacgacc gcctgcacac acggtgccct gggagccgag
    10021 actgttagtc acccactcga aaacggtacg gttgtagtaa tccagaccac gcaccagacg
    10081 ctggtttacg gtgtaagcga tccccgcgct ctcaagcagt ttgcacagac cggcaaaatg
    10141 ctcacgagat tcctcgtcca gataatcacc taatgccgga gcgtcgttga gaagcgcctg
    10201 cacttccgga ttttttgaat ccagcacgcg cagcgggtta gtgtacatgc ggcgtttgca
    10261 gtcttcgtcc agcttttctt tatgctgctc aaggaatgcc accagcgcat cgcggtaatt
    10321 ggcgcgtgct tccagcgaac cgatagaatt cagctcaaga gttacgtgtt cggaaatacc
    10381 cagcgcgcgc caccagcggg cggtgagcat aatcagttca gcgtcgatat ccggaccttg
    10441 cagaccgaaa acttcgcagc ccaactgatg gaactgacga taacgccctt tctgcggacg
    10501 ctcgtgacgg aacatcggcc cgatatacca cagacgctgt tcctgattgt acagaagacc
    10561 atgctcgatg ccggcgcgta cacagcccgc cgtcccttca gggcgcagag tcaggctgtc
    10621 gccattgcga tcctcaaagg tgtacatctc tttttcaacc acgtcggtga cttcaccaat
    10681 cgcacgtttg aatagcgggg tctgctctac aatcggcaag cggatttcac tgtaaccgta
    10741 gctgccgagc acgtttttca gtgtgccttc aatgcgctgc cagatggccg tttcgccagg
    10801 caggtaatcg ttcatgccgc gaatggcttg aatgtttttt gccacgttta ttctctttct
    10861 gaatataaaa atgaaccctc aacgcttccc tcaatgtttc gggagccatg cgggttcaat
    10921 catacacggg aagcgaggcg cttcccatca cgttattatt tttcaacctg ctgaacgtca
    10981 attcgacgcg cttcgtccag ctgactggct ttcgcacgaa tgcgtgcctc cagctggtcg
    11041 atcatatcgt tgttgtccag acggtctttg cgcacgccat cttcatagag accgcttttc
    11101 ttgttgccgc cggtgacgcc gagtgtagaa accagcgcct cacccgggcc attcaccacg
    11161 cagccgataa tcgaaacgtc catcggagtg atgatatctt ccaggcgttg ctccagcgcg
    11221 ttaactgtac cgataacatc aaattcctga cgcgaacagg tcggacaggc gatgaagttg
    11281 atacctcgcg aacggatacg aagcgatttc aaaatatcga aaccgacttt gatctcttcg
    11341 accggatcgg ccgccagtga tacgcgcagc gtgtcgccga tgccttcaga cagcagcaga
    11401 cctaaaccaa tggcggattt tactgccccg ctgcgcgcgc cacccgcttc ggtgatcccc
    11461 agatgcagcg gctgatcgat ttgttttgcc agcaaacgat aagactcaac agcgaggaag
    11521 acgtcagacg ctttcacgct gactttgaac tgatcgaagt tcaggcgatc gagatgatca
    11581 acatggcgca tggcggattc cagcaacgcc tgcggcgtcg gttcgccata cttttcttgc
    11641 agatcttttt ccagcgatcc ggcgttaacg ccaatacgga tcggaatgtt tttatcgcgc
    11701 gcacagtcaa ccaccatgcg aatacgctct tcattaccga tattgccagg gttaatacgc
    11761 agacaatcga cgccgtattc cgctactttc agcgcaatgc gatagtcgaa gtggatgtca
    11821 gccaccagcg gcacgttaac ctgctgtttg atgagtttga acgcttctgc cgcgtccatc
    11881 gtcggtacgg agacacggac gatatcagcg ccaacgcgtt ccagcgcctt gatttgattg
    11941 accgttgctt cgacatctgt cgtacgcgta ttggtcatgg actgtacggc gatgggagca
    12001 ccatcgccaa tcggcacatt cccaacgtaa atacgtgttg attttctacg ttgaattgga
    12061 gcctggttat gcatgaaaaa tctcccgcgt tacccgtctg ttactgcgcc ggtgattgtt
    12121 cggcattgag ggtcagacgc gcaacctggt tagttctgat aaaacgactc agatcgacag
    12181 gtttcccttg atactggatc tgtactgcgg ctggcgcacc aattttcagt ttgtacggtg
    12241 cctggccggt taagttcaaa ttaccgtctt tacgctgcat accgctaaac aattttttac
    12301 cggtagcatc agtgacctcc agccagcaat cggcagtaaa gttcatcacc agcgcattcg
    12361 gatcagccac cggcgtggtc acgccagcct gatcggttgg caacggcgca gcaccatctg
    12421 gcgttgttgc tgccgttggt gccggggtcg cggcggtatc aacatttgcc tgcgaaggcg
    12481 aaacaaccgc attctgttgc ggatcaacag ctggtgctgg cgcagttacg acaggtgttt
    12541 gcgtgttggt tgcggtagta tccacagacg ctggcggcgt ggacgttgta gccgggtctg
    12601 tagttgtcga cgtatttaac ggaacactct gcccctgctc gctattactg ctcagttccg
    12661 ccgaagattg atcggccata gtggtgatct cttcctgctg agctttgtgg tcttgccacc
    12721 accaggcacc gctcaggccg ataaccacaa acaacaccag ccaggtgaag gtcatcagcc
    12781 agccgtcgcg ttttttgcgg cgtttaccga gggaaaaact ctgcatcggc gcaacttttg
    12841 cagcccgtag tggagcctgc ttttccagcc ctggcagcag ttcttcttct ggaatatgta
    12901 ccagacgcgc ataagagcgg atatatccgc gcaggaatgt tgaagcaaga tcggcgggtg
    12961 ccttatcttc ttcaatgtcg cgtaccgtgg aaaccttcag gcaaagtcgc tcggcaacgg
    13021 cctgctgact aagtcctagt tgttcgcgag cattacgcag gcgagcgccg gtagtaagtg
    13081 cttcattttg gtcgtgcgtg gcttcagtat tcattcgctg caggtacgtt taaatgagaa
    13141 ttgagatgcc ggtgaaccat catgctcacc cacaccgcga aacatccgtt aagttaaccg
    13201 ttatcataca gtataagacc gtctgtgtgc tcgtgacaaa gcccacattc actaaggcta
    13261 acttactgtt gcatcgttac atactgcctt aaagtcagca aaaacgcacc gttaatattg
    13321 accagacaat tgcaacttaa tacttcattc ataaaacatt acgccacggt acataaagta
    13381 accgtggcgt aatggctatc agaccgcttt aatgtcgatg gcttcaccct gcatccgttt
    13441 acgcagggta cgtttcgtac ggtcgataac atcgcccgcc aactgaccac aggcagcatc
    13501 gatatcatca ccacgcgttt tacgcacaat agtggtgaaa ccgtagctca tcagcacttt
    13561 tgagaaacgg tcgatacggc tgttcgagct gcgtccatac ggcgcagccg ggaacgggtt
    13621 ccacgggatc aggttgatct tacacggcgt atctttcagc agttccgcca gttggtgcgc
    13681 gtgttcagtg ccgtcgttaa cgtggtcaag catcacgtat tcaatagtga ctcggccctg
    13741 attggcgttg gatttctcca gataacggcg caccgcagca aggaacgttt cgatattgta
    13801 ctttttgttg atcggcacaa tttcgtcacg aatttcgtcg ttcggcgcgt gcagggaaat
    13861 tgccagcgca acatcgatca tatcgcccag tttatccagc gctggaacta caccggaagt
    13921 ggaaagcgtc acgcgacgtt tagacagacc aaaaccgaaa tcatcaagca tgatttccat
    13981 cgccggaacg acgttgttca ggttgagcag cggctcgccc atgcccatca tcactacgtt
    14041 agtgattggg cgctgaccgg tgacttttgc tgcgccgacg attttcgccg cacgccacac
    14101 ctggccaata atttccgata cccgcaggtt gcggttaaag ccctgctggg cggtggaaca
    14161 gaatttacac tccagcgcac accccacctg cgaagagacg cagagcgtag cgcggtcgtc
    14221 ttccgggatg tacaccgttt cgacacgctg gtcacccacg gcaatcgccc atttaatggt
    14281 gccgtcagat gaacgctgtt cttcaaccac ttccggtgcg cggatctccg ccacctcttt
    14341 cagtttgccg cgcaacactt tgttgatgtc ggtcatctca tcaaaattgt cgcagcaata
    14401 gtgatacatc cacttcatca cctgatcggc gcggaagggt ttttcaccta aatctttaaa
    14461 aaactcccgc atctgctgac gattgagatc cagcaggttg atttttccat ctttggtggt
    14521 gacgttttca ggtgtgacta attgttcaga catatgctat tccggcctcg ttattacacg
    14581 ttatggcccc tggagggttg aaaaaagaaa cgccccggtg agcggctgct cgtccggggg
    14641 cgctgcattg tacaaattct ggcgcacgga tgccacgttt gcacgcggca tttacgaaat
    14701 tattaacggg tgcgcgggca cacttcgcct tcgccgaaga aataagcgat ttcgcgagcg
    14761 gcagattcga cggaatcaga accgtgggta ccgttttcgg tcaggctgtc agcgtaatca
    14821 gcgcgcagag taccggccag tgcgtttgcc ggattggtcg cgcccagcag atcgcggtga
    14881 cgctgaacgg cgttttcacc ttccagcacg gaaaccacga tcgggccaga ggtcatgaat
    14941 tcaaccagac catcaaagaa cggttttcca tcgtgttcag cataaaagcc acgtgcctgt
    15001 tcaacggtca ggtgcagcat tttggtgccg acaattttga accctgcagc ttcaaagcgc
    15061 gcaaagatat taccaatgac gttttttgct accgcgttcg gtttgataat ggaaaaagta
    15121 cgttcaatag ccatttttac ctctgtaaat tgttctgttg ttgtctgtac cagcgtacga
    15181 gatggcgcgg attataatga gcaacagagc cgttgactat tgatgaaggt aacatttttt
    15241 taaaataaaa tgagttttag caacaactcg acttttatgg tgttctcatc ccataacgat
    15301 gacgttggca ataaagacaa ttttgcgcat tttgctgaaa agtaagaaag gatttttgac
    15361 aggttttaca gactttgcga ttagcatgaa gattcacaat ttttgccggt agaatcgtca
    15421 attcaacggt cattgcctga tgaaaacaaa cagcaataca attttggcat ccgttacaca
    15481 aggcatcatt tatttgtaaa tggccttcgt taagcgaaaa gacctgttgc tcgcaggccc
    15541 gccagcatgc accgcaaagc tgacactttt gaggatccat catcgggcga gcatcactaa
    15601 tctggggcca taaataatgt aaccgacgta atcctggttg tatttgtgtc atctgaacat
    15661 ccgcacgctg cacatggaac aaacgacgcc gggaaacatt cacctcactg gtatgtggag
    15721 ataccacaga ccagccttcc tcgccatatt gccgtaagca ttcgtttaac agcaataatg
    15781 cgcgctccca tgccttcgcc tgactcagat caatcgcgat cgctgtgatc ccctgctctt
    15841 tatgccatag caaaagttct tccagcgtcg gcgcaatagt ggtgaaaggt gcgaccaggg
    15901 tgtgattgca aaaataacgt aaaggggaat taacgccacg aatagcctct tgcggacaaa
    15961 taaactggca aaccgcacaa tcaatacatt gttccgcgtc gaccgctatg ccctcttctg
    16021 tcagcgttag cgcctgtgcc gggcaagcct cgacacaggc atgacatgtt gcaaaacgaa
    16081 agcgttggcg cgcgcattcc gcgccaatcg ttatgttatg acttaacatt agccgatact
    16141 aaaaaagaca aaacgcagca taacttcact ccccactagc attacgctac cgaataacgc
    16201 cgccgggata ttctttttac aaccaccgac agcaaagaac atcatcccaa tgacggaaat
    16261 tccccagctt agtgtcagta tgggttttaa ctgtcccagc atcgctaatg gcgcatgtgg
    16321 caatgtggct atctgcatcg catttgccgt taaatcccct atccacaacg gctgaaccaa
    16381 caaacgcagc agaaccagga gagcgatcac caacacgcat ccattacgca gggcatcaat
    16441 ctgcggcaaa attccgctaa tacgaaacac tgctaccagc gctgaaccga gaattcccac
    16501 tgtacctaag aacataatca gcgtgttgta atgctgccag gtgataatcg acgtatggat
    16561 gtaaacttgc gccatgcaga aaacatcaac aatcccgacc actgccgcta gcataagcag
    16621 gagttgccag ccgcctttgc gtaacatgac cagtaatgtg gataaaccca atagcgccag
    16681 atacagcgca ccaaagataa tttcccgact cagccatgaa ctggaaaagt ggctcagcgc
    16741 atgcatcgca ttaagtggat aacccaggtg taaggtagaa atcagtaacc ctacggcacc
    16801 tgccaaaaaa gccaacagta atggcggccg cataaccggg cgggcgctgg aggtgattaa
    16861 aatatgacga tgaagaaacg ccagccacaa cgtcataccg accgatgcct gtaacaacag
    16921 cgtaaaagcc attaacggta attcatgcat gatgtgacgc ctccttttcc gcgccctgat
    16981 gcggtttaat caccagattt ggctgcgtaa tcgacgaatc aggtaaacct ttcacatcac
    17041 tgacatcgcc atacttttca cgcaactcat caatagggcc gaacttaatc gcccccaacg
    17101 ggcacgttgc cacgcaaatg ggttgttctc ctttagcttg cagatcaata cagaaatcgc
    17161 atttcgacat ctgcccggtc tgctcattca tttgtggtgc gccgtaagga caggaccagg
    17221 cgcagtaacc gcaaccgacg cacttatcag tattcacgcg cacaataccg tctccgggac
    17281 gcttatgcat cgccatcgtc gggcaatttt tggtacagat cggatccgcg caatggttac
    17341 aggaaacaga aagggtataa gcaaagacat tatgactcac accgccctct ccagtggcga
    17401 tgaagccacc tccgttcact tcatacacgc ggcgaaaacg tcgcccaacc tcaaggttgt
    17461 ttttgtcttt acaggccacc tgacacgctt tacagcccga acaacgcgaa gagtcaataa
    17521 agaaccctaa ttgtttgtcg ctgacagatg caaattcttt aaattggctc atgctttctc
    17581 aacctccacc agcattgttt gatgcgaatt ccctttcgcc agcgccgtaa tgcgggtcga
    17641 actcagtaca ttggcacagc ctccgcgatc gataccttgt gcatccggtt gccaccatgc
    17701 ccccgcctgc atagcaacta cgccagggat gatacggggg gttatttcca ccgggataat
    17761 gctcacacca cgatcgttat aaatttttac gctatctcct tcgctaatcc cgcgctgttt
    17821 tgcatcttgc ggattcagcc agagtttttg cgtctgaact tcttgcagcc acgggttagc
    17881 atactgggtg gaattagcac ggtttttccc tttccaggtg atgagctgca acgggtattt
    17941 cgccgtcagt ttatcttctg gcccttcaaa ggcagggaca tagtgcgaaa gcgcgggaat
    18001 ttctggatct tgcatatcaa aaagacgctt agagaagatc tcaattttgc cggaaggcgt
    18061 cgggaaagga ttattctgcg gatcgcgaat atttgcttcg aaagcgatgt gcggcgcgct
    18121 tttgaaaaga tgtcgacgtg ttttttgcag ggttgcgaag tccggcaaat tttcatccgg
    18181 catcgccaga cgagtttgct cccagatatg ctcgatccat tgcttctcat cacgtccttg
    18241 actgaactcg gcttcgacac ccagtttttt cgccacgtca cgcaaccagt cataatcggt
    18301 gcgacgttca aaatccggct caatcggttt ttctgacaga ataagatagc tcgcagttcc
    18361 ccatgtttcg ccgatattcc agcgttccat aaagctggtt tccggcagca acacatcggc
    18421 atatttcgcg ctcggagtca tgaacagatc gctgagtacg atgaattcta ttttagattc
    18481 gtcttcaagg agcttcgcgg cctggtggac gtccgggttc tggttcgcca gataattacc
    18541 tgccagtgag aacagcaggc ggatattact gtcgagttta tcgacacctt taagcccatc
    18601 ttgcggcgta actttagaag catcatccgc cgcctgcatc cagttcataa tcgagatttt
    18661 cgcttgtacc ggattttctg gcatatctgg tccaacgcag aatttacgat tagaactacc
    18721 gccgtaacct gccgcccagc cacctttgat gccgacgtta ccggtaatcg aggccagtaa
    18781 agttgaacct cgggcggtgc gttcgccgca aatatgtcgc tgtggccccc atccctgaat
    18841 caacgctgcg ggttttgtag tggcgtaatc gcgtgccagt tgacgaatgg attgtgctgg
    18901 aacgtgggta attttttctg cccattccgg cgttttgtgt atcccatctt ttgcgccaaa
    18961 aaggtaggca accaacgatt cgttggctgg tacgccttcc ggcatggagt tttcatcaaa
    19021 accgagagta tatgtgtcga taaatgtctt atcgtggaga ttctcgctga taatgacgta
    19081 catcatcgcg tccatcaacg cattgtccgt ggtcggcaac agcgggatcc actgatcggc
    19141 aagtgaagat acggtgtcgg aatagcgcgg atccaccacg ataaaacgtg taccgttttg
    19201 cttcattttt tgaaaatagt aattggtgtg accgaatatg gtttccgttg ggttatgccc
    19261 ccagagaatc accagtttgg tatccagaag agtatccatc gagttaccgc tcgcggccac
    19321 gccatacgtg taaggcgtgg ccgccgcagt attacccaga ctaactgagt gataatattc
    19381 aagataacca ccggttaaat ttaacagccg acgcgccatc gcatcgccag aaaatgttcc
    19441 accagaaaca gccgtcccga catggacata acgtgaagct gggccatatt gttgcgtgat
    19501 ccttttcagg ttatcggcaa ttaaggtcgt cgcctcatcc catgagatac gctcaaattt
    19561 accctcgcct cgtttaccta cccgcttcat cggatatttc aaacggtctg gatgataaac
    19621 aaatttacga tagccgcgac cacgaacaca ggcgcgcata atcggcattt gtggatccaa
    19681 ttcgttatcg ggaagcgtgg taatttgcgt caccacgccg tcgcgcatat gcgcccgaat
    19741 atcgcactta ccaccgcagt caaatgtgct acaggtgggc acgactttat cagctactgg
    19801 agctgtaact tctggcgacg catctgggga ttgcgccttt gcggtagaag aaataaaagg
    19861 caaagtgata agcgcggaag tcgcttgtaa aaaactacgt cgtgatactt gcggaaatgt
    19921 aatgcgttcc ttttcgtctg gcgtatccat cttgcgttcc cgaaaatgaa aaaataaata
    19981 ttcacgtatt tattcgag
//
LOCUS       DAZFDC010000086        19862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFDC010000086 DAZFDC010000000
VERSION     DAZFDC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.086"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            283..783
                     /gene="tssB"
                     /locus_tag="ACHJ48_003198"
     CDS             283..783
                     /gene="tssB"
                     /locus_tag="ACHJ48_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO4708694.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDEMNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            818..1042
                     /locus_tag="ACHJ48_003199"
     CDS             818..1042
                     /locus_tag="ACHJ48_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708695.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1093..2484
                     /gene="tssC"
                     /locus_tag="ACHJ48_003200"
     CDS             1093..2484
                     /gene="tssC"
                     /locus_tag="ACHJ48_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO4708696.1"
                     /translation="MSLQEEELVSSHAGQPEQESSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWY"
     gene            2575..2988
                     /gene="tssE"
                     /locus_tag="ACHJ48_003201"
     CDS             2575..2988
                     /gene="tssE"
                     /locus_tag="ACHJ48_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO4708697.1"
                     /translation="MILDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2992..4842
                     /gene="tssF"
                     /locus_tag="ACHJ48_003202"
     CDS             2992..4842
                     /gene="tssF"
                     /locus_tag="ACHJ48_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO4708698.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4806..5888
                     /gene="tssG"
                     /locus_tag="ACHJ48_003203"
     CDS             4806..5888
                     /gene="tssG"
                     /locus_tag="ACHJ48_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO4708699.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTTFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5913..7193
                     /gene="tagH"
                     /locus_tag="ACHJ48_003204"
     CDS             5913..7193
                     /gene="tagH"
                     /locus_tag="ACHJ48_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO4708700.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHAPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7190..7714
                     /gene="tssJ"
                     /locus_tag="ACHJ48_003205"
     CDS             7190..7714
                     /gene="tssJ"
                     /locus_tag="ACHJ48_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO4708701.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7717..9048
                     /gene="tssK"
                     /locus_tag="ACHJ48_003206"
     CDS             7717..9048
                     /gene="tssK"
                     /locus_tag="ACHJ48_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO4708702.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAELLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9053..9814
                     /gene="icmH"
                     /locus_tag="ACHJ48_003207"
     CDS             9053..9814
                     /gene="icmH"
                     /locus_tag="ACHJ48_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO4708703.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALHGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9823..12600
                     /gene="tssH"
                     /locus_tag="ACHJ48_003208"
                     /gene_synonym="clpV"
     CDS             9823..12600
                     /gene="tssH"
                     /locus_tag="ACHJ48_003208"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO4708704.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLAPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYERSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESEQDNTGAVPADETDREQPEETAETVSPVQRLAQLT
                     AELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKG
                     QDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLT
                     TINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLN
                     LFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEALYPVLADF
                     FKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSR
                     VTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQN
                     DESVTKDETVL"
     gene            12597..13340
                     /gene="vasI"
                     /locus_tag="ACHJ48_003209"
     CDS             12597..13340
                     /gene="vasI"
                     /locus_tag="ACHJ48_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO4708705.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDHLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13345..14756
                     /gene="tssA"
                     /locus_tag="ACHJ48_003210"
                     /pseudo
     CDS             13345..14756
                     /gene="tssA"
                     /locus_tag="ACHJ48_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            14893..18327
                     /gene="tssM"
                     /locus_tag="ACHJ48_003211"
     CDS             14893..18327
                     /gene="tssM"
                     /locus_tag="ACHJ48_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO4708706.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQLLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRHREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVTQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENITLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18338..19747
                     /locus_tag="ACHJ48_003212"
     CDS             18338..19747
                     /locus_tag="ACHJ48_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO4708707.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLKNNTIPTIKKALNFNN
                     IHEYKGVLNGEFNLFNTKW"
     gene            19713..>19862
                     /gene="tssD"
                     /locus_tag="ACHJ48_003213"
     CDS             19713..>19862
                     /gene="tssD"
                     /locus_tag="ACHJ48_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO4708708.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNR"
BASE COUNT         4661 a         5473 c         5496 g         4232 t
ORIGIN      
        1 taattcaata agatacacaa aaaactctat atttaaatta ttgccacaat acacctgacg
       61 cgaattgcac aggttcataa aatctattta acgcttgcag aatacccctc tttacgatcg
      121 ataaaaagtc cttttcttat ttattttgaa gcgtaaaatg cggggtgatg aaggagtggt
      181 gattttattt tcaccgccat caagacggca tttccgatat tttttgcggt acgaatatgc
      241 cgtgttcatg gcctgtacga gataacatga cggatcactt ttatgagcaa aaaatttgaa
      301 ggcagcgtcg cgcctcgcga acgtattaat atatcgtatg tcccaaaaac agatggccag
      361 acggctgaag tggaacttcc gcttaacatg ctcgttgtcg gcgataccgg aaatacgcag
      421 gaaacatcct cactggatga acgtcaggca gtttccgtta acaagcacaa tttcggtgca
      481 gtgatggctg aagcagccat tggcctcaat tttaccgtcc ccgccacgct gaagggaagc
      541 acaacggacg atgaaatgaa tgtggcgctg aatatcaagt cactggatga tttttcacca
      601 gatagcgtgg cccggcaggt tccggaagtg aataaattgc tggaactgcg tgaagcactg
      661 actgcactga aggggccgat gggtaacctt ccggcattcc gtacccagct ccaggcattg
      721 ctggaaaatg aagagtcccg cgagcagttg ctcaaagaaa tcggccaggt gagcaataaa
      781 taaccaccac tatttcatta aacagggtta tccaggcatg acggcacaca aaaatatctc
      841 cgggacatac cacctctctg ttgcagacat tctgcaggtg gtttaccagg tatgtttcag
      901 cccgtccgtt gaaatcaacc aggatggtgt ggctgcgctg attacaacac tggatcgccg
      961 tatcagtgat ctattggatg agattatcca tttttgtgag tttcagcaga gcgcgtcgca
     1021 ctggcagcgc gtacttcact gacggcgaaa agccggtttc agaaacacag ctaaaacaga
     1081 aggattttca gtatgtcttt acaggaagag gaactcgttt ccagtcatgc cgggcagcct
     1141 gagcaggaat cgtcgctgct tgatcaaatc atggcacaga cccgtattca gcccggttcg
     1201 gaaggttatg acgttgcccg ccagggtgtg acagccttta ttgccagcat cctgcaatcc
     1261 actgcatctg ccgaaccggt caataaactg gccgtggaca gcatgattgc agatattgat
     1321 gaacgcatca gccgccagat ggatgtcatc atccatgcgc ctgcctttca gcaggtcgag
     1381 tcgttctggc gctcactgaa aaccatggtg gatcgcgttg atttccgtga aaacatcaag
     1441 gtcaacgtgc ttcatgtcac caagcaggaa cttctggaag actttgagtt tgcgccagaa
     1501 attattcagt ccggatttta taagcacgtt tattcgtccg gtttcggaca gtttggcggt
     1561 gaacccatcg ctgccgtgct gggcgcttat gagttcaaaa ataccgcgcc tgacatgaaa
     1621 ctgctgcaat acgtcagtgc cgtgggcgca atggcgcacg caccgttcct gtcctccgtt
     1681 tccccggagt ttatggggct gaactcgtgg accgaactgc ctaatatcaa agatctttat
     1741 gccatctttg aaggtccggc ctacaccaaa tggcgcgccc tgcgtgactc ggaagattcg
     1801 cgctatttag ggcttacggc accccgcttc ctgcttcgcc agccctattc accaacagat
     1861 aaccctgtta agaactttaa ctattacgaa gatgtcagcc agaaccacga agattatctg
     1921 tggggtaata cggcctggat gctggcatgc aatatcgcag acagttttgc caaataccgc
     1981 tggtgtccaa acattatcgg tccgcaaagc ggcggcgcag tgaaagatct gccggtgcat
     2041 ctgttcgaaa cgatggggca gattcaggcc aaaatcccaa ccgaggtgct ggtcaccgat
     2101 cgccgcgaat ttgaactggc tgaagagggt tttatcaccc ttaccatgcg taaagactct
     2161 gataatgcag cctttttctc tgcaaactcg gtacaaaaac cgaagcactt tccgggaaaa
     2221 gatgcagaaa ccaactataa gctgggtacg cagcttcctt atctcttcat catcaatcgg
     2281 ttagcgcact acatcaaggt gttgcagcgt gaacaactgg ggtcatggaa agaaaggagc
     2341 gacttagaaa gagagcttaa tacctggatc cgtcagtatg ttgccgacca ggaaaacccg
     2401 cctgcagacg tgcgcagccg caaacctctg cgcgctgcca aagttgaggt tatggatgta
     2461 gaaggcgaac ccggctggta ctaggttgcg ctaagcgtga gacctcattt taaatttatg
     2521 ggggcaaatt ttgagctttc cctggttggc cggttagaca gggagtaaca caccatgata
     2581 ctggatcggg aaagaaccac aggttctctt tttgagcgta tggaagcctc ttcggccaga
     2641 aaccggcagg gaggcagtat tcattcgctc aggcaatcca ttcgccagaa cctaagaaac
     2701 attcttaata cccggtccgg tagttgccgg ggtgcgcctg agctgggcat tgatgaaccg
     2761 gaaggggctg aaaacttccg cgaatccatg agtcgggcca tcgagcaatg tatagaacgc
     2821 tatgaacccc ggatctcaca cgctgaagtg caggcagtgg tttcatccgc ctccagcccg
     2881 ctggatatga cgtttcatat tacagcctgg gtgacattta acgaaacgca tgaggtgctt
     2941 gaatttgata tggcaccgaa tggcagccag cattaccggg tggactgaca aatggaattt
     3001 gaagaacgct attttcggga agaactcgat tacctgcgcc agcttagcaa gctgctggca
     3061 acggaaaaac cccatctggc ccgcttcctg gccgaaaaag atgcggatcc ggatattgaa
     3121 cgcctgctgg aaggggtggc ttttcttacc ggcaatctcc gccagaaaat tgaggatgaa
     3181 ttcccagaac tgacgcacgg gcttattaag atgctatggc ctaattacct gcgtccggtt
     3241 ccggcaatga cccttattga atatacgccg gatatggata agtcttctgt accggtgtta
     3301 atcccccgta atgagcagtt tacaaccaac gccggggaaa tcagagttga tgaagtgctg
     3361 ccctctgatg ctaaaaagga ggagccgcct ccctgtacct tcaccctctg ccgggatatc
     3421 tggctgctgc ccgttcgcct ggagcagatt gaaaaccgca gtacgacccg taatggtgtt
     3481 atcaacatca ccttttcggt cgcaccggga acggacttcc gcacgctgga tctgaacaaa
     3541 cttcgcttct ggctcggcaa tgacgacaac tatacccgtg accagcttta tttatggttc
     3601 tgcgaatact tgcagggtgc cgacctgact gtgggtgaac agcatattcg cctgcctgag
     3661 tttatgctaa aagctgtcgg ttttgagccg caggatgcca tgctgccctg gccgaaaaac
     3721 gtccacagcg gctaccggat ccttcaggag tatttctgtt accccgatgc gtttctcttt
     3781 tttgatcttt gtggttgtcc ggctttgcct gacggattgc aggcggaatt ctttaccctg
     3841 caactgcgtt tttcgcgccc tttgcccgtg gacatccggc tgcgccgcga ttccctgcgc
     3901 ctgtattgcg cacctgccat taatttattt atccaccatg cagaagccat cacgctggac
     3961 aaccggcggg cagactatcc gctggttccc agccgccatt acccacaaca ttacgatgta
     4021 ttttccgtta acagtgtggt gagccaggtc caggatatgt tcaggaaaaa agatctgggg
     4081 cgtcctgttt cgacgcaggc cgcgcgccag tggccagcct ttgaaagttt cagccatcag
     4141 atggaataca gccggaagcg ggaggtggtg tactggcatc accggaccaa aacatccctg
     4201 ttccatcgcg gctttgatca tacccttgcc tttatacatg ctgatggcag ttatccgtca
     4261 gacgaatctc tgctcagtaa tgaagtggtt tcggtatcgc tgacctgtac caaccgtgag
     4321 cttccgtcac aaattcgttc cggcgatatc accggcacaa ccggtaaaaa tgcggctgtt
     4381 gcttcctttc gcaacattac ccgcccgacg caaccactct ggccggtcat tgatggcagc
     4441 ctgcactggt ccctgctctc cgccatgaac ctgaattatc tgtcattact ggatacggac
     4501 gcgctgaagc aggtcatcgc caactttgat cgccacgcaa tccaccatcc gcagacggcg
     4561 cggctgtcac aacaaaagct ggatgccatt gagcgtctgg agacccgccc cgttgatcgc
     4621 ctgtttacgg gtattcccgt ccggggactg gcctccacgc tgtatctgca cccggagccg
     4681 tttgtctgtg aaggggaaat gtatctgctc ggtacggtgc tttcgcattt tctgtcgctg
     4741 tacgccagcg ttaactcatt ccacatgctg accgttgtga acacagaaag ccaggagaca
     4801 tggaaatgga cggaaagaat cgggcagcat cctcttatct gagtccggga aatcccccgg
     4861 cagataaaga gcagaatgat ccgctggcac aggttttcca taatgcctgc tcttacaatt
     4921 tttttgcgat ggcggagctg ttgcaccgcc tggcaaaggg tgaaaagggg acgccagaat
     4981 tatccctgcg cgacgatccg gcacaggaaa ccctgcgttt cagcgccgat gccagccttg
     5041 ccttcccgtg cagtgatatc agtgcgctga aacgggatac gtcaggggca ttcaggatga
     5101 ccactacctt tatggggtta caggggagcc agtcgcctct ccccggttat tacctcgatc
     5161 acctggcctg gaaagcggta catgaacaaa gtccggtagg tgattttctg gatatgttca
     5221 gccaccgcct gacccagttt gtctggcata tctggcgtaa gtaccgctat cacatcagtt
     5281 ttcgtaacgg cggtgtggat gcgttctcgc agcgcatgta ctcccttgtc gggctgggcc
     5341 accgccagct tcgcgataag ctggcgatta atcacagcaa aatgctggcg tattccggga
     5401 tcctggcaaa tccgggacga tccccggaaa ttatctgtgg cctggtgtcc cactgctttg
     5461 atttgtcaga agtgacgctg caaaactggc aacgtcgcaa ggtggatatt gaaccggatc
     5521 agcaaaacag cctggggagc tattctctta aaaatggcga aaaactggcg ggaagatcgg
     5581 tactgggcaa ctttgttctg ggtacacgag tacccgatct cagcggaaaa ttccagctca
     5641 gtattaccag cctgacccga aaacagttcc tctcttttct gccgtccggc gaaaacttcc
     5701 tgccactgac gacgttcgtg tccttcatcc tgcgcgatca gcttgcctgg gacttacatc
     5761 tggggctggc gccggagcag gtgggcgcaa tgcgcctggg cgataacaaa agtgcgctgc
     5821 tgggctggac cagcttcctc ggcacaccgg aagaacgacc ctcagtcacc atcagggtac
     5881 ggtcgtaaat tatgataatc acggagcaga atatgccgga agagaaacta cagacgcttt
     5941 cattgcaggt cattaacggc agtgagctgg aaagcgggcg ggcggcgcgc tgtctgttca
     6001 cacagcaggg aaatgtcggc catgcccccg aatgccactg gtcggtacag gatcgtcagc
     6061 agagcattcc ggcccaggct tttaccgtta tcctgcacga tggcacattt tgtctacgcc
     6121 cgcagacggc acaactgtgg ctgaatcagg caaaagtcac agcaacatca gacctgatac
     6181 agttgcgcca gggcgatgag atccagatcg gacggctgat ggtgagggtt catctgaacc
     6241 ggggagatat tccccattac gatgaggaaa tggccactcc cgaaaccatc gttaccaatc
     6301 gcgatatgct cacggatacc ctgctatcaa cggagggtgc gccacactat ccgggaatga
     6361 ctcaccggca ccagcttgca gacaccgtgg taaatggttt ttctgccgat ccactccagg
     6421 cacttcagtc cgaaagcctg attaccacgg gcgatccgct ttcaggcatt gcggctgtcc
     6481 ggccatcggc accgctgtcc gatccggcaa gtaatggggg gatcaatact ccgtttatgg
     6541 atctgccgcc catttatgcc agccctggcg atcataatga tgacatctct gcggcagaaa
     6601 tggcgcaacg ccaccttgcg gtcaccccct tactgcgcgg tcttggcggc tcgcttaccg
     6661 tgagcaattc cgacgatgcg gatgattttc tggaggaggc cggacgaacg ttacaggccg
     6721 caataaaagg tctgctcgat ttgcagcagc agcgtaacag cctctcagac aaacatttgc
     6781 gcccgctgga agataacccg ctgcgcctga acatggatta cgccaccgcg ctcgacgtga
     6841 tgtttgccga aggtaaaagc ccggtacatc tggcggctcc cgccgccgtc agtgaaagcc
     6901 tgcgcaatgt ccgccaccac gaagaagcta accgggcggc gattgtggag tcgcttcgtg
     6961 tcctgctgga tgctttctca ccacaaaatc tgctgcgccg ctttgtgcag taccgccgca
     7021 gccatgaact gcgccagccg ctggatgatg ccggagcatg gcaaatgtac agccattatt
     7081 acgaagaact ggcctccgat cgccagcagg ggtttgagat gctgtttaac gaggtctacg
     7141 cccaggtcta tgaccgggtg cttcgtgaaa aacagcggga gccggaagca tgaacaggaa
     7201 ggcgtttctg gcctgcgtgc tgatgtgcgt catattaacc ggctgcgaaa cggcgaaaaa
     7261 aatcagcgag gtgatcaaaa acccggatat ccaggtcggg agcctgaagg agcaaccttc
     7321 ggaaataacc gtcaccctgc tgactgaacc ggacaccaat accaatgcgg agggtgaatc
     7381 agccgccgtg gatgtgcaac tggtgtacct gactgacgac tcaaaattgc aggcagccga
     7441 ctatgaccag atagccagta cgccgctacc ggatgtgctg ggtaaaaact atatcgacca
     7501 ccaggatttc aacctgttgc cggacaccat caaaaccctg ccgccgataa agctggatga
     7561 gaaaacgcag tttattggtg tcgtggccta tttctccgat gaccaggcca ctgaatggaa
     7621 acaaatcgag acggtggaag gaaccggcca ccattaccgc ctgctggtgc atgtacgtca
     7681 aagcagcatc gaaatgaaaa aagaggatga ataattatgg caaccacccg caacaaggtg
     7741 atgtggcagg aagggatgct gatgcgccca caccatttcc agcaacagca gcgttacaac
     7801 gactacctgg ataaccagcg tttccgggcc atgaatgatt tatcctgggg atttaccgaa
     7861 ctcaccctca acaatgaact gctggcgcag ggtaagatca tgattgacag cgcgtcaggc
     7921 acactgcctg acggcaccgt cttttctatc cccgaccagg acgcactgcc cgatccgctg
     7981 cacccgcaaa attttccgga cgaaagaagc cgcaatattt acctcgctct gccggtcgcc
     8041 agtgatgtga gaaatgaaat cagcgacggg cggcgaatcg ggcgttaccg gctgaattat
     8101 gccgatgtcc gggatctgca ttcagaagaa ggcgacgcgc gaacactgac gctgggacaa
     8161 ctgacgccgc gcattatgag cggtgcagaa gatatgagtg cctatattac gctcccgctt
     8221 tgccgtatca gcgatcgcca tgccgacggc tccctgacgc tggatgacga ttttatcccc
     8281 tcctgccaga atattcaggt cagtaagaaa ctgcgtgttt atctcaaaga ggtacagggg
     8341 gccattggcg gacgggcaag cgatctggca aaccgtattg gctctccggc gcagagcggc
     8401 atcgcggatg tggcggaatt tatgatgttg cagttgctta accgtaacca gacccggttt
     8461 acccatcgcg cccgccgatc ccagctccac ccggaagatt tctaccttga tcttgccgag
     8521 ttgctgggtg aactgatgac ctttacagag ccgtcacgcc tgccctgccc gcttgatgtg
     8581 tatgatcatc atgacctgac caaaacattt aaaacactgt taccggaagt caaacgggcg
     8641 ctgcataccg tactgtcgcc aagagcggtc aatctgccgc tgcatctgcg tgacggcatc
     8701 tggcaggccg atgttcatga ctcggaactg ctacagtctg ccacctttgt actggccgtg
     8761 gcagcaaata tgcctgtcga tcagatccag cgtcagttta tccagcagtc gaaaatttcc
     8821 tcgccggaaa aaatccgcaa tatggtcagt gtgcagatcc cgggtattcc tctgcgcgca
     8881 ctgatggtgg ccccccgcca gcttccctac cattccgggt tcagctattt cgaactcgac
     8941 aagagcggcc aggcctggac agaaatggct gccgccgggg ccgttgcact gcatgtatcc
     9001 ggcagtttcc cggatctgaa catgcaactg tgggcgataa gagggtaaga cgatggatga
     9061 ggggtcactg tctctgccac catttaccgg atacgacgaa aaaagcctgc gcgactacca
     9121 tctcgcgctt catggcaaca gcctgaatcc gatgatcgac gcggcgacgc cgctactcgg
     9181 tatggtaatg cgcctttcca ccatgaacag ccagacgatg ccggagcatc tttttgctca
     9241 ggtggtgaca gatgttcagg ccgtggagca actgcttcag gaacagggct acgaaccggg
     9301 cgtgattatc tcgtttcgct acatcctctg cacgtttatt gatgaagcgg cgctcggtaa
     9361 cggctggtcg aacaagaacg agtggatcaa gcagtcattg ctggtccatt tccacaacga
     9421 agcctggggt ggcgagaaag ttttcatcct gctggagcgc ctgatccgtg aacccaaacg
     9481 ttaccaggat ctgctggaat ttctctggct ctgtttttct ctgggatttc gcgggcgcta
     9541 caaagtggca gtacaggatc agggggaatt tgagcagatc taccgccgtc tgtaccacgt
     9601 tctgcataaa ctgcgcggcg atgccccttt cccgctgtta caccaggaca aaaaaaccca
     9661 aggcgggcgt tatcagctca tcagtcgcct gactgttaaa catatttttt gtggtggcgt
     9721 tgtcgtgctg gcgttgtttt acctgttcta tctgttgcgg ctggacagcc agacgcagga
     9781 catcctgcac cagctaaaca aattacttcg ataaggacgt ttatgatcca gattgatctt
     9841 cccacgctgg taaaacggct gaacctgttc tcccgccagg cgctggagat ggccgcctca
     9901 gaatgtatga gccagcaggc agcggaaatt accgtcagcc atgtgcttat tcagatgctc
     9961 gccatgccac gcagtgacct gcgggttatt acccgccagg gcgatattgg catggaagag
    10021 ttgcgccagg cgttgacggt agagaactac acaaccgccc gttctgcgga cagctacccg
    10081 gcgttttccc cgatgctggt tgagtggctt aaagagggct ggctgctggc gtcggctgag
    10141 atgcagcaca gcgaactgcg cggcggcgtg ttgctgctgg ccttgctgca ttcgccgctg
    10201 cgttatatac cgcctgctgc cgcccggctg ttgaccggca ttaaccgtga ccgtctgcaa
    10261 caggactttg tgcagtggac acaggagtcg gcggaatcag tcgtgccgga tgcagacggt
    10321 aaaggcgcag gcacactgac ggacgcctct gacaccctgc ttgcccgcta cgccaaaaac
    10381 atgacagaag acgcccgtaa cggcaggcta gccccagtac tgtgccgcga ccacgaaatc
    10441 gacctgatga tcgacattct ctgccgccgc cgtaaaaaca acccggtggt ggtgggcgaa
    10501 gcaggcgtgg gtaaaagcgc gctgattgaa gggctggcgc tgcgcatcgt ggcaggccag
    10561 gtgccggaca aactgaaaaa caccgatatc atgacccttg acctgggcgc attgcaggcc
    10621 ggggcatcgg tgaagggtga gtttgaaaaa cgcttcaagg ggctgatggc ggaggtcatt
    10681 tcctctccgg tgccggtcat tctgtttatc gacgaagcgc ataccctgat tggcgcgggc
    10741 aaccagcagg gcgggctgga tatctccaac ctgctgaaac cggcgctggc gcgtggcgaa
    10801 ctgaaaacca tcgccgccac cacctggagc gagtacaaaa aatacttcga gaaagacgcc
    10861 gccctgtcgc gccgcttcca gttagtgaag gtcagcgaac ccaacgctgc cgaagccacc
    10921 attattctgc gtggtctgtc ggcggtctat gaacggtctc acggcgtgct gattgacgat
    10981 gatgccttgc aggccgcagc aacgctgagc gaacgttatc tctccgggcg tcagttaccg
    11041 gacaaagcga ttgatgtgct ggataccgcc tgcgcccgtg tggccatcaa cctgtcgtcg
    11101 ccgccgaagc aaatctcggc gctgaccact ctgagccacc agcaggaggc ggaaattcgc
    11161 cagcttgagc gcgagcttcg catcggactg cgtaccgaca catcacggat gaccgaggtg
    11221 ctggtgcagt atgatgaaac gctgacggcg ctggatgaac tggaagcggc ttggcaccag
    11281 cagcagacgc tggtccggga gattatcgcg ctgcgccagc agttactggg cgtggcagag
    11341 gacgatgcgg cgccgttgcc ggacgcagat actgtggagg atacgcagcc agagtcagag
    11401 tcagagtcag agtcagaaca ggataatacc ggtgccgtac cggctgatga gaccgacaga
    11461 gaacagccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc ccagctcact
    11521 gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt cgataaaaaa
    11581 cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct gtcgcagaat
    11641 gaaatgtcgg tcatcaccga cctgccgaaa tggctgggcg acaccatcaa aggccaggac
    11701 ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct gcgtcgtccg
    11761 ggacgcccgc tcggtgcgtt cctgctcgct ggccccagcg gcgtgggtaa aaccgaaacc
    11821 gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac catcaatatg
    11881 tccgagttcc aggagaaaca caccgtttcg cggctgattg gctcccctcc gggctatgtc
    11941 ggctatggcg aaggcggcgt gctgactgaa gccatccgcc agaaacccta ctcggtggtg
    12001 ctgctcgatg aagtggaaaa agcgcacccg gatgtcctca acctgttcta ccaggcgttt
    12061 gacaagggcg agatggccga cggcgaaggc cgcctgattg actgtaaaaa catcgtcttc
    12121 ttcctcacct ccaacctcgg ttaccaggtg attgtcgaac acgcggatga cccggaaacc
    12181 atgcaggaag ccctctatcc ggtgctggcg gacttcttta aacctgccct gctggcgcga
    12241 atggaggtgg tgccgtacct gccgctgtcg aaagagacgc tcgccaccat tatcgccggg
    12301 aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgcagaagt ggtcattgaa
    12361 ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg cgcgaggatg
    12421 ctggaatctg tcatcgatgg cgacatgcta ccgccgctct cgctgctgct gttgcagaaa
    12481 atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg cgcatttacg
    12541 gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac ggttttatga
    12601 gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg acgggcttac
    12661 ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc gccgtcgcca
    12721 ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt cggcgggagt
    12781 ccgcggcgct ggagcggctt gactgctatg accacctgct ggcaccgctg tccccgtcag
    12841 ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga ggcctggacc cgcgccacag
    12901 agcaggaaaa acgccgcgag ggcaacacca cagaactgct ggtgacgcag gtgccggggg
    12961 agcgcccgac ggtggtgata accaccccgg ctatcggcca tgttccgccg cgcccggtgc
    13021 tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg caccctctgg
    13081 atgtccatga tatcgccgtc actctgaatg ccgacagccg ggcattgcgc agccactggt
    13141 ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gctgtccggg attgatgaaa
    13201 ttaagcaact gttcggcgca aaaacgctga cagtggatac cggcgcagat aacgccgcag
    13261 gaaagctgac ctttaacatt gacgggctgg caagggcgat tgcccccctg cgcgatgcct
    13321 gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa tgtctggata
    13381 tcgcacctgc tggaaaacct gccggaagaa aaactggcat cggcgctgaa agatgacaac
    13441 ccggactggg agtatatcga cggcgaaatc gtcaagctgg ggtctctggc ccacgctcag
    13501 cttgatattc ccgaactaca acgcaggggg ctacagcttc tggcttctga aagcaaagac
    13561 ttcaggctac tggcacacct gctcagaacc ctgcaacatg ccggtgatcc actgctggca
    13621 ctgcacctgc taacgctata cgtggaacat tactggactg tggccgcgcc gcagaatatg
    13681 gcgcataaaa aacgctttgc cagccagatc attaaacgtt ttgaaacggt attgaaggct
    13741 tttcacaaaa cgctgccaca acgcagcgcg atactctgct gggtgagctg gcgaaactgg
    13801 cgcagtgctg gcagtcacat aacgtcccgg aactggcaca ggctaccgat gatctttttg
    13861 ccctgtacca gcgtacgttt aatcgtgcgg ctcctgctcc ggtccccact ccggcggcct
    13921 ccggtagttc accacaaacc accgtcacgt ctgaaagcgg cgtgacgcaa cccagtgctc
    13981 cggctcccca aatcgccatc gacagtcacg acgacaaagc ctggcgcgac acgctgttaa
    14041 aagtggcggc tattttatgt gaacgccagc cggactcgcc gcagggctat cgcctgcgcc
    14101 gccatgccct gtggcaatcc atcaccagta caccccaggc ggaaagcgat ggacgtaccc
    14161 cactggctgc ggtctctgcc gatatggtgg cggattacca gtcccggctt gccagcgcgg
    14221 atatggcgct gtggcaacag gttgagaaaa gcgtattgct ggctccttac tggctggacg
    14281 gtcactgtct ttctgcacag acggcactgc gtctgggtta caaacaggtg gcagacacca
    14341 tccgcgatga ggtcatccgc ttccttgagc gtctgcccca gcttaccggg ctgctgttta
    14401 atgaccgcac accgtttctc agtgagcaga cgaaacaatg gctggctgct tcgcccgacg
    14461 gcaaagttgc accggttgcg caaatcggtg aggaatcgca ggcagccaga gcctgttttg
    14521 ctgggcaggg tctggaggcg gcgctgcgat atctggacat gctacccgaa ggcgatcccc
    14581 gcgatcagtt tcaccgccag taccttgccg cacagttgac ggaggaggcg gggctgatac
    14641 agcttgcgca gcaacagtac cggatgttgt tgatgatagg gagtcagatg atggtgtctg
    14701 actgggagcc atcattactt acgcagcttg aacaaaaatt cacggcagaa caataaaaga
    14761 gaacaggagt ttttgtgttc agattaccaa caccccgact actcagcgga ctcaaatcag
    14821 ccctgcgacc ggcgatgccc cggtttaaag tctccgcttt ctggctgctg atactggcgt
    14881 ggatttttct gcttgctgat actggcgtgg atttttctgc tggtatggat ctggtggaaa
    14941 ggcccgacgt ggacgctgta tgaagaacag tggctcaaac cactggcgaa ccgctggctg
    15001 gcaacggcgg cgtgggggat tattgccctg gtgtggctta ccgtccgggt aatgaagcgc
    15061 ctgcaactgc ttgaaaaaca gcaaaaacag cagcgcgaag aggccattga tccgctcagt
    15121 gtggagctga acgcccagca gcgttacctt gaccgctggc tgctgcgcct gcaacgccat
    15181 ctcgacaacc gccgtttcct gtggcagtta ccgtggtata tggtcatcgg cccggcgggc
    15241 agtggcaaaa cgacactgct gcgcgagggg tttccgtcag acattattta tgccccggag
    15301 ggcgcgcgcg gcacagaaca acgcctgtac cttacgcccc atgtcggtaa acaggcggtg
    15361 atctttgata tcgacggcac actctgcgct cccgctgatg cggatatcct gcatcgccgt
    15421 ctgtgggaac atgccctcgg ctggctgaaa gaaaagcgcg cgcgccagcc gctgaacggg
    15481 attattctga cactcgattt acccgatctg ctgaccgcag acaaacgcca ccgcgagcat
    15541 ctgttacaga cgctgcgcag ccgcttacag gatatacgcc agcatcttca ctgccagtta
    15601 ccggtttacg tggtactgac ccggcttgat ttattacagg gttttgccgc cctgttccag
    15661 tccctgaaca gacaggatcg cgatgcgatt ctgggcgtca cgttcacccg ccgtgcccat
    15721 gaaaatgatg actggcgaac agagttgaat gctttctggc agacatgggt ggatcgaatg
    15781 aatctggcgt tgccggatct gatggtcacc cagactcaca cccgcgcgtc tttattcagt
    15841 ttttcccgcc agatgcaggg aagccgtgaa ccgctggtgt cactgcttga gggtctgctt
    15901 gacggtgaaa atatgaacgt gatgctgcgt ggtgtctatc tcacctcttc gcttcagcgc
    15961 gggcagatgg atgatatatt cacccagtct gccgcccgcc agtaccgact gggcaataac
    16021 ccactggcgt cctggcctct ggtggacacc gcgccttatt tcacccgcag cttgttcccg
    16081 caggcattac tcgcagagcc taatctggca acggagagcc gcgcctggct gatgcgttcc
    16141 cgtcgccgcc tgacggtttt ctctgccacc ggcggcgtgg cggcactgct gctcatcacc
    16201 ggctggcatc actattacaa cggtaactat cagtccggca tcaccgtgct taagcaggcc
    16261 aaagccttta tggatgtgcc gcctccacag ggggaagatg actacggcaa cctgcaactg
    16321 ccgctgctga acccggtacg cgatgccaca ctggcctatg gcgactgggg cgaccgcagc
    16381 cgtctggccg atatgggact gtaccaggga cgacgtatcg gaccttatgt ggaacagacc
    16441 tatctgcaac tgctggagca acgttacctg ccctcgctgt ttaacgggct ggttaaagcg
    16501 atgaatgccg cgccgccgga gagtgaagaa aaactcgcgg tgctgcgcgt gatgcgaatg
    16561 ctggaggaca aaagcggacg taacaatgag gtggtgaagc agtatatggc aaaacgctgg
    16621 agcgaaaagt tccacggcca gcgcgatatc caggcacaac tgatgtccca tcttgactac
    16681 gcgctggctc atactgactg gcacgcagag cgtcaggcgg gcgacggtga cgccatcagc
    16741 cgctggacgc catatgacaa gcccgtggta tcagcacaga aagaactgag caaactgcct
    16801 gtctaccagc gggtttacca gagcctgaaa acgcgggcgc tgggcgttct tcctgccgac
    16861 ctcaatctgc gtgaccaggt agggccaacc tttgaccagg tgtttacatc tgccgatgac
    16921 aacaaactgg ttgttccaca gtttcttacc cgttacggcc tgcaaagcta ttttgtaaaa
    16981 cagcgcgatg aactggttga actgacggcg atggattcct gggtacttaa ccttacccgc
    17041 agcgtgaaat acagtgacgc cgaccgcgcg gaaatccagc gccagctcac cgagcagtat
    17101 atcagcgact acaccgccac ctggcgggcc gggatggaca atctgaatat ccgcaatttt
    17161 gagtccatcg gacaactgac cggggcgctg gagcaggtta tcagcggcga ccagcctttg
    17221 cagcgggcgc tgaccgtgct gcgtgacaac acacagccag gcgtcttttc tgaaaaactc
    17281 tctgccaaag aacgggatga ggccctggca gagccggact accagttact cacccgtctc
    17341 gggcatgagt tcgccccgga aaacattacc ctggcagtac agaaagacaa agaaagcacg
    17401 atgcaggccg tgtatcagca actcaccgag ttgcaccgct acctgctggc aatccagaac
    17461 gcgcctgtac cagggaaatc ggcgctgaaa gccgtgcagt tacggcttga tcagaacagc
    17521 agcgatccga tattcgccac ccgccagatg gcaaaaacgc tgcctgctcc gctcaaccgc
    17581 tgggttggca gactgactga ccaggcctgg catgtggtga tggtggaggc tgtacattat
    17641 atggaagtgg actggcgcga cagcgtggtg aaaccgttta acgagcaact ggcaaataac
    17701 tatccgttta atccgcgttc tgcacaggat gcctcactgg atgccttcga acgcttcttt
    17761 aaaccggatg gcatactgga taccttctac cagcagaacc tgaagctgtt tatcgataat
    17821 gacctgagtc tggaggatgg cgataacaac gtcattattc gcgaagatat tattgcgcaa
    17881 ctggaaactg cgcagaaaat ccgtgacatc ttcttcagca aacagaacgg tctgggaaca
    17941 tcctttgccg tggaaacggt atcgctttca ggcaataaac gccgcagtgt actgaacctt
    18001 gacggtcagt tagtcgatta cagccagggc cgtaactata ccgcccatct ggtctggcct
    18061 aacaacatgc gcgaaggcaa cgaaagtaag ctgacgctca tcggcaccag cggcaacgcg
    18121 ccgcgcagta tcagcttcag cgggccgtgg gcgcagttcc gcctgttcgg ggccggacaa
    18181 ctgaccggag tacaggatgg caactttacc gtgcgcttta gcgtggacgg tggcgcgatg
    18241 acctaccgtg tgcataccga cacggaagat aacccgttca gcggtgggtt gttcagccag
    18301 tttggtctgt cagacacact gtactgagga ggcagggatg aatagtaacg tactgacaca
    18361 aactatcgtt accggcagtg acccgcgcgg gctgccggaa ttcagcgcca tccgcgagga
    18421 aataaacaaa gccagccacc cgtcacagcc tgagctgaac tggaaactgg tggagtcgct
    18481 ggcgctggcg atttttaaag ccaacggtgt ggatttacac accgccacct actatacgct
    18541 tgcccggaca cggacacagg gactggcggg attctgcgaa ggtgcggaac tgctggcggc
    18601 aatggtaagc cacgactggg ataagttctg gccgcagggc ggcccggcgc gtactgaaat
    18661 gctggactgg tttaactccc gaaccggcaa tattctgcgt cagcaaatct cctttgcgga
    18721 atccgaccta ccactgatct accgcacaga gcgggcattg cagcttatct gcgataagct
    18781 ccagcaggtg gaactgaagc gcgttccgcg cgtggagaat ctgctctatt ttatgcagaa
    18841 cacgcgtaaa cggcttgaac cccagctgaa gagtaacact gagaacgccg cacagaccac
    18901 ggtcagaacg ctgatttatg ccccggaaac acaggcatct tccacaccag aagcggtagt
    18961 gcctcccctg cccggcctgc ctgagatgaa agtggaagtg cgcagtctga cagagaatcc
    19021 cccacaggcc agtgtgataa agcaaggcag tacggtaaga gggtttatcg cagggatcgc
    19081 ctgttcagtg gctgtcgcct cagcattgtg gtggtggcag gtctatccgg tgcagcagca
    19141 actgttacag gttaacgaca ccgctcaggg cgcagcaacg gtgtggatgg cctcacctga
    19201 actcgaaaac tatgagcgca ggctgcaaca acttcttgat acctccccgg tacagccgct
    19261 ggaaaccggg atgcagatga tgcgtgttgc cgacagtcgc tggccggaaa gcctgcaaca
    19321 gcaacaggcc tcgacacaat ggaatgaggc actcaaaacc cgcgcacaga gtagcccgca
    19381 gttgcgtggc tggttgcaga cccgccagga cttacatgct tttgcagatc tagtgatgca
    19441 gcgcgagaaa gagggactaa ccctttccta tatcaaaaat gtcatctggc aggcggagcg
    19501 gggactgggg caggaaacac ccgttgagtc tctgttgacg cagtaccagg atgcccgtgc
    19561 gcagaagcag aatacagatg cgctggaaaa acaaattaat gagcgactcg aaggcgtgtt
    19621 aagccgctgg ctgctgctga agaataatac gataccgacg ataaaaaaag cattgaattt
    19681 caataacatt catgaataca aaggagtttt aaatggcgaa tttaatttat ttaacactaa
    19741 atggtgataa tcaagggctc atttcaagtg gttgttcatc acaaccatcc attggaaata
    19801 aagctcaaac agcacacatt gatcaaatta tgatttatga attcatgcat ggattaaatc
    19861 ga
//
LOCUS       DAZFDC010000087        19644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFDC010000087 DAZFDC010000000
VERSION     DAZFDC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.087"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..450
                     /locus_tag="ACHJ48_003214"
     CDS             <1..450
                     /locus_tag="ACHJ48_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO4708709.1"
                     /translation="NTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQ
                     GEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQY
                     GKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(488..1387)
                     /gene="nhaR"
                     /locus_tag="ACHJ48_003215"
     CDS             complement(488..1387)
                     /gene="nhaR"
                     /locus_tag="ACHJ48_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO4708710.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADV
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1453..2619)
                     /gene="nhaA"
                     /locus_tag="ACHJ48_003216"
     CDS             complement(1453..2619)
                     /gene="nhaA"
                     /locus_tag="ACHJ48_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO4708711.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3205..3357
                     /gene="mokC"
                     /locus_tag="ACHJ48_003217"
     CDS             3205..3357
                     /gene="mokC"
                     /locus_tag="ACHJ48_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO4708712.1"
                     /translation="MKQHKVMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3461..4591)
                     /gene="dnaJ"
                     /locus_tag="ACHJ48_003218"
     CDS             complement(3461..4591)
                     /gene="dnaJ"
                     /locus_tag="ACHJ48_003218"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO4708713.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4680..6596)
                     /gene="dnaK"
                     /locus_tag="ACHJ48_003219"
     CDS             complement(4680..6596)
                     /gene="dnaK"
                     /locus_tag="ACHJ48_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO4708714.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6973..7377
                     /gene="yaaI"
                     /locus_tag="ACHJ48_003220"
     CDS             6973..7377
                     /gene="yaaI"
                     /locus_tag="ACHJ48_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO4708715.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNALAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7403..8116
                     /gene="msyB"
                     /locus_tag="ACHJ48_003221"
     CDS             7403..8116
                     /gene="msyB"
                     /locus_tag="ACHJ48_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO4708716.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSSYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8265..8831
                     /gene="satP"
                     /locus_tag="ACHJ48_003222"
     CDS             8265..8831
                     /gene="satP"
                     /locus_tag="ACHJ48_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO4708717.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8866..9453)
                     /gene="mog"
                     /locus_tag="ACHJ48_003223"
     CDS             complement(8866..9453)
                     /gene="mog"
                     /locus_tag="ACHJ48_003223"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO4708718.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9568..10521)
                     /gene="tal"
                     /locus_tag="ACHJ48_003224"
     CDS             complement(9568..10521)
                     /gene="tal"
                     /locus_tag="ACHJ48_003224"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO4708719.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10800..12230
                     /gene="yaaJ"
                     /locus_tag="ACHJ48_003225"
     CDS             10800..12230
                     /gene="yaaJ"
                     /locus_tag="ACHJ48_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO4708720.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            12300..13076
                     /gene="yaaA"
                     /locus_tag="ACHJ48_003226"
     CDS             12300..13076
                     /gene="yaaA"
                     /locus_tag="ACHJ48_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO4708721.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(13229..13525)
                     /gene="yaaX"
                     /locus_tag="ACHJ48_003227"
     CDS             complement(13229..13525)
                     /gene="yaaX"
                     /locus_tag="ACHJ48_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO4708722.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(13739..15025)
                     /gene="thrC"
                     /locus_tag="ACHJ48_003228"
     CDS             complement(13739..15025)
                     /gene="thrC"
                     /locus_tag="ACHJ48_003228"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO4708723.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(15026..15958)
                     /gene="thrB"
                     /locus_tag="ACHJ48_003229"
     CDS             complement(15026..15958)
                     /gene="thrB"
                     /locus_tag="ACHJ48_003229"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO4708724.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVESAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15960..18422)
                     /gene="thrA"
                     /locus_tag="ACHJ48_003230"
     CDS             complement(15960..18422)
                     /gene="thrA"
                     /locus_tag="ACHJ48_003230"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO4708725.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18448..18561)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18503..18586)
                     /locus_tag="ACHJ48_003231"
     CDS             complement(18503..18586)
                     /locus_tag="ACHJ48_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO4708726.1"
                     /translation="MKRISTTITTTITTTITITITTGNGAG"
     gene            complement(18800..19486)
                     /gene="yjtD"
                     /locus_tag="ACHJ48_003232"
     CDS             complement(18800..19486)
                     /gene="yjtD"
                     /locus_tag="ACHJ48_003232"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO4708727.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLAILIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         4756 a         5156 c         4781 g         4951 t
ORIGIN      
        1 aatactattt tacaggtaaa tttaatgacc tgtggttttg aagatcaatc catcgacact
       61 ggacgatttg tactgtctcg tatcaataat gaaaattatc agtttcatca tataagattt
      121 gactgcatcc agggcgagca aggtgaatta gtttttgaac ccagtgacgt tgaatattac
      181 tttgaaccag tgacgataca ggagtcagga accagcatat taaaaaatga gttagaaaac
      241 agtgaggacg gtgcgggtca aataggcttt caactctcca atgatggtac gaatgaaatc
      301 cagtatggta aaagcaatta ctactctttt cagcatcccc atgaaggaag taatcagatt
      361 cctctattta ttcgcccgcg tacatatgga aacaatgtct caagcgggca aataatgagc
      421 cgagtcaaaa tcgtggtaat gtacaactaa tctatctccc ccaccttaac tttgggggag
      481 ctgccgatta acgcaccgct ggactaaaaa gcgcagaata atccgtattg cagattcgct
      541 gtaccgccgg gtgctgaatc atccgctcag caaaaatagc atggtactct tccatcacat
      601 tctcgacgcg accaatttct acgacagttt tatcggcata aaagtcatat gcataaagcg
      661 ttggggcaac gaagattgca ttgtgcatcg caccaaaagc tttcatcaaa gcggcatcat
      721 caaactcgcc aaggatttct acgtttaatc cctgggagtt aaaccagtta agtaatttgc
      781 gccctaacat tgaacgtcgc ccaggaatca aaagccgccg ttcttcaaga caagctggga
      841 acggtttttc cggcggtgga tttgtacacc agaaactcac gccacattcg ccaattctca
      901 cggagaacag gccttcctgc tgcgtagaat ctatcggaca gtcggaaatg atcatatcca
      961 gtttatgctg gcttaattgc tccagcagca tttcgtgggt ggattcgaag cagcgaagat
     1021 gaatcggctc gccttctact actgcagcat tcagcacgct actgaccaga cgtttggaaa
     1081 gtacatcagc cacgccaacg tcaaacaata aattggattc tttgcgatag ttcacgatat
     1141 ccagcatttc ctggctcaag gtaaacattt tatcggcata gcgatagacc agctccccca
     1201 gctcgctggg ctcgagacca cgtcccttgc gcttaaataa tttgccttgc aggcgctctt
     1261 ccagcgcccg aatctgtccg gtaatggttt gtggtgttaa ataaagcgcc tccgctgcgc
     1321 caaccacaga accttcttta taaacatgcc agaagtaata caagtggttg taattgatat
     1381 gagacatgct catttctctc cctgataaca atgaaaaggg agccgtttat ggctccccgg
     1441 taaaccgtcc tgtcaaactg atggacgcaa acgaacgcgt aaccagctgt atccaattac
     1501 cgccgaagag atagaaccga ccaggatacc gagtttcgcc cagttaatca gttctggatc
     1561 tacgctacca aaggccaggc tggcaataaa gatagacata gtaaaaccga taccgcacag
     1621 gatccccacc gccataattt gctgataagt cgttccctca ggcagatgcg ccagtttcaa
     1681 acgcagcgcc aaccagcaga acagactaat ccccagcggt ttgccaatca gcaagccagc
     1741 gatgatcccc aatggcagaa tggaggtcaa gccatccagc gtgacgcctt gcagtgaaac
     1801 gccagcatta gcaaatgcaa acagtggtaa aatcagatac gccacccacg ggtgcaacac
     1861 atgctccagt cgcttcgctg gagaacgccc atgcttctct ttcaaaggaa taaagaagcc
     1921 gacaattacc cccgccaggg ttgcgtgaac ccccgatttc aacactgcag tccacaacac
     1981 tacgccaacc agaatataga cgcccgtgcg gcgtacacca cacagattca ataccgcgag
     2041 tacagcaatt gctacagccg cgacgccaag agaggccatc gataagtcat tagtgtagaa
     2101 caatgcgatg ataatgatgg ccccaagatc gtcgataata gccagagcca tcaaaaagat
     2161 cttcagcgct aacggaacac gacttcccaa tagcgccagc acaccaagtg caaaggcaat
     2221 gtcagtagcc gccgggatcg cccacccttc gcgggtaatc ggatcggcat agttaaaggc
     2281 cagatagagt aatgccggca caatcatccc accaatagca gcgataactg gaaatgcggc
     2341 ctggcgtaag ctggctagcg atccttgcat cagttcacgt ttaacttcca gaccgactaa
     2401 caggaaaaat accgccatca gcgcgtcatt tatccataac agcatgtttt tgttgatttc
     2461 gagtgaacca acccggagct gaaccggcgt ctccagaaag tcgtgatacc atccactggt
     2521 tgcgccgctg ttggccatca tcatcgccag gatagcggca atgataagaa taatgcctcc
     2581 cgaggcatca ctgctaaaga atcgatgcag atgtttcact ttttatttct ctttcaggtg
     2641 aatagatcgt ttttactatt ttaccccgca cgaatcatcg ttaaaatcag attatagtgt
     2701 aggaataatt cgttttttac gatctataga agaatttgcg ccatcagcgg tgagatgcgt
     2761 ttttgacggc tgtaagcgct gacgggtgac cctaagccct gataatcata gcttaaggtg
     2821 aggatttctg agggattaat taggaaaagt aataattaat ttgttgtcag tcggcgatgt
     2881 ttgttaggcc aatatttcaa aaataatcca gcttgtcgtc aataattgcg atgaagagat
     2941 aaattaaagt taaataatta aatcattaaa aaattacgcc ataaaaatgc gaggcgtctt
     3001 ccggattaga atcgataaaa cagaatgggt taaagagagc gatataatag cggcgggtgc
     3061 ttgaggctgt ctgtctcagg cattagctga acggcagata gagaaaagcc ccgagtgata
     3121 ttttaccatc aacccgaggc cccctatatg ctgaacacat gtagagtgcc tcttactgac
     3181 cgtaaggtca aggagaagag agcaatgaag cagcataagg tgatgattgt cgccctgatc
     3241 gtcatctgta tcaccgccgt agtggcggcg ctggtaacga gaaaagacct ctgtgaggtt
     3301 cacatccgaa ctggccagac ggaggttgct gttttcacgg cttacgaatc cgagtaagag
     3361 caacggcggg gagtgatccc cgccacttta caggtgctcg catatcttca acgcacccta
     3421 tttttaccca ggcctgccca cgggcaggct tttggggagg ttagcgggtc aggtcgtcaa
     3481 aaaacttctt cacaccatca aagaagctct ttgagcgcgg gctgttgtgc tcgccggttg
     3541 ggccaccgaa gctttcttgc agctcttgca gcagctgttt ctgcttctcg ttcaggccta
     3601 ccggtgtttc gacgacaacg cggcacagca aatcaccctg tgcgccaccg cggacagact
     3661 tgacgccttt accgcgcata cggaatagct taccggtctg ggtttcgcca ggcactttca
     3721 gtttgacgcg accatcaagg gtcggtactt cgatttcacc acccagcgcc gccatagcga
     3781 agttgatcgg gacttcgcaa tacaggttgt tgccttcacg ctcgaaaatc gggtgctgtt
     3841 taacctgaac ctgaacgtac agatcgcctg ccggtgcgcc gtgttcaccc gcttcacctt
     3901 cgcccgcaag acggatgcgg tctccagtgt ccacccctgc cgggatttta acggacagcg
     3961 ttttgctgcg ctcaacacga ccatgaccat gacatttgtt gcacggatct ttgatcagcg
     4021 taccgcggcc ctgacagtgt ggacaggtct gctgcacggc aaagaaaccc tggcgcatct
     4081 gcacctgacc agaaccatga caggtcggac aggtctgcgg ctgtgtacct ggttttgcac
     4141 cgctaccgtg gcaaacgtca cactcttcca gagtcggaat gcggatctct ttggtcacgc
     4201 cacgtacagc ttcttcgagg gtgagctcca tgttatagcg taaatcagca ccgcgcgccg
     4261 cacgttgacg accacgtccg ccgccaaaaa tatcgccgaa aacgtcacca aaaatatcgc
     4321 tgaagtctgc gccgccgcca aaaccgccgc cgcccatgcc accttgctca aacgcagcat
     4381 gaccatactg atcgtatgcc gcacgttttt gcgagtcggt cagaacttca taagcttcct
     4441 tgatctcttt aaatttcgcc tcggcctctt tgtcaccctg gttacggtcc gggtggtatt
     4501 tcatggccag gcgtttgtag gcctttttga tttcacgctc ttccgctgtt ttggaaacgc
     4561 ctaaaatctc gtaataatct tgcttagcca tcttttttaa attgccccta gatgaatgca
     4621 cgggcggaga ggaaattccc cttcgcccgt gtcagtataa ttacccgttt atagggcgat
     4681 tattttttgt ctttgacttc ttcaaattca gcgtcgacaa catcgtcatc tttcgcgttg
     4741 tttgcagaag catcagcacc ggcagtctgc tgctgggcat gttgctgctg ggcgatttcc
     4801 atcagtttct gggaaacctg tgccagttcc tgcattttcg cttcgatagc ggctttgtct
     4861 tcacctttca gagcagtttc cagtgcagtc agcgcagact cgatagcagt tttgtcgtca
     4921 gccggcagtt tgtcgcctgc ttcttcaacc tgcttacggg tgctgtgcag cagatggtcg
     4981 ccctggttgc gagtctgtac cagctcttca aacttacggt cagcttcggc gttagcttcg
     5041 gcgtcgcgta ccattttctg gatttcatct tcgttcagac cagaagacgc cttgatggtg
     5101 atcttctgct ctttaccgct gtttttatct ttcgcggaaa cgtgcaggat accgtcagca
     5161 tcgatatcga aggtaacttc gatctgcggc atgccgcgcg gtgccgggtt gataccatcc
     5221 aggttgaact gacccagaga tttgttgtca gccgcacgtt tacgttcacc ctgcagcaca
     5281 tggatggtta ccgcagactg gttgtcttca gcggtagaga acacctggct gtgcttggtc
     5341 gggatagtgg tgtttttcgc gatcagcgtc gtcatcacac cgcccatggt ttcgataccc
     5401 agagacagcg gggtaacatc cagcagcagt acgtctttta cgtcaccagt cagaacacca
     5461 ccctgaacag cagcaccgat tgctacagct tcgtccgggt taacgtcttt acgcggctct
     5521 ttaccaaaga actcagcaac tttcttctga accattggca tacgagtctg accaccaacg
     5581 aggataacgt cgtcgatatc agatacggac aggccagcgt cctgcagcgc aactttcagc
     5641 ggctcgatgg aacggttaac cagatcttca accaggcttt ccagtttcgc acgagtcact
     5701 ttgatgttca tgtgtttcgg accggtcgcg tctgcagtga tgtacggcag gttaacgtcg
     5761 gtctgctgag cggaagacag ttcgattttc gctttttctg ccgcttcttt caggcgctgc
     5821 attgccagcg gatcgttgcg caggtcaatg ccctgatctt tcttgaattc ttcaaccaga
     5881 tagttgatca gacggctgtc gaagtcttca ccacccaggt gggtatcacc gttggttgcc
     5941 agaacttcga aggttttttc gccgtcaact tcgtcgattt cgataataga aatatcgaaa
     6001 gtaccaccac ccaagtcata aaccgcgata gtacggttgc cagtaccttt gtccagaccg
     6061 taagccagcg cagctgcggt cggttcgttg atgatacgtt ttacttccag accagcgata
     6121 cggcctgcgt ctttggttgc ctgacgctga gcatcgttaa agtatgccgg tacggtgata
     6181 acagcttcag ttaccggttc acccaggtaa tcttcagcgg ttttcttcat ttttttcagc
     6241 acttcagcag aaatctgcgg cggtgccatt ttctggcctt taacttcgac ccatgcgtcg
     6301 ccgttatcag cagcaataat tttgaacggc atgatggaaa catcacgctg tacttcttcg
     6361 tcctggaagc ggcggccaat caggcgttta atcgcaaaca gggtgttttg cgggttcgtc
     6421 actgcctgac gtttagccgg ctgaccaacc agagtttcac catcctgggt ataggcaatg
     6481 atagaaggcg tggtgcgatc gccttcggcg ttctccagca cacgaggagt ggtgccatcc
     6541 ataatcgcta cacaagagtt ggtagtaccc aggtcgatac caattatttt acccatctaa
     6601 acgtctccac tatatattcg gtcatcatgt ggttgtgagt ctgtaatagg ggcgaaacgt
     6661 ctggtttcaa ctgcccaatt tcattttttt gcagactcac tcactgcggt tgactactaa
     6721 atggggtcgt aacccacgtc atcaaggggg agatgcaaaa attttttgtg cttttgcgca
     6781 gaaatttcgc gatatggcag gacaagacgt aaaattgaca gcaattttat tcatataaag
     6841 aatgaatcgc agtcaaggta ttcatcaaaa aattgtaata aaaagaaaag attacgtgcc
     6901 tgaatcttct ctttatcagc agtaaactag tgggtattca tccccctacc tcttcccact
     6961 aagagaatcc ttatgaaatc cgtttttacg atttccgcca gcctggcgat tagcctgatg
     7021 ctgtgctgca cggcgcaggc aaacgaccat aaactcctcg gcgtcattgc aatgccgcgt
     7081 aacgaaacca acgctctggc gctgaaactt cctgtttgtc gcattgtgaa acgcatacaa
     7141 ctctctgccg accatggcga tttacagtta agcggtgcat cggtttattt caaagccgcc
     7201 cgtagcgcca gtcagagcct gaatattcct tcagaaataa aagaagggca aaccactgac
     7261 tggatcaaca ttaacagcga taacgacaat aaacgctgcg tctcaaaaat caccttttcc
     7321 ggtcatacgg tgaactcatc ggatatggcc acgctgaaaa ttatcggcga cgactaacgc
     7381 cagaatatag aagccacaaa aaatgaatgt taattacctg aatgattcag atctggattt
     7441 tctccagcat tgtagtgagg aacagttagc aaatttcgcc cggctgctca cccataatga
     7501 aaaaggcaaa actcgcctct ccagcgtact gatgcgcaac gaactgttta aatcgatgga
     7561 aggacatccc gagcaacatc gccgcaactg gcagctgatt gctggagaat tacagcattt
     7621 tggtggcgat agtatcgcca acaaactgcg cggacacggt aaattgtatc gggccatttt
     7681 gctcgatgtt tcaaaacgat tgaagctgaa agccgacaaa gagatgtcta cgtttgaaat
     7741 tgagcaacag ttactggaac aatttctgcg taatacctgg aagaaaatgg acgaggaaca
     7801 taagcaggag tttctgcacg cggtcgatgc cagggtgaat gagctggaag agctgctgcc
     7861 gctgctgatg aaagacaaat tattggcaaa aggcgtgtcg catctgcttt ccagccaact
     7921 gacccgcatt ttacgcaccc acgcagcaat gagcgtactt gggcatggtt tgctgcgcgg
     7981 cgcggggctg ggaggccctg taggcgcggc actaaatggg gttaaagcgg tcagcggcag
     8041 ctcctatcgc gtgacgattc cagccgtact gcaaatcgcc tgcctgcgcc ggatggttag
     8101 cgccactcag gtctgatacc ggaaaccggg gaaaattttc attttcccct caaaagatcg
     8161 tagacactgc cccactggct gattattatg ccgcgccctg aaaacactac agttattcag
     8221 ggaaattatt tcaccattca ttcgatgatg atttttgagg aattatgggc aacactaagt
     8281 tggctaatcc ggcaccgctg ggcctgatgg gcttcggcat gaccaccatt ctgcttaacc
     8341 tgcacaacgt aggttatttc gctctggacg gtattattct tgccatgggc attttctacg
     8401 gcggcatcgc gcaaattttt gctggtctgc tggagtacaa aaaaggcaac actttcggtt
     8461 taaccgcatt cacctcttac ggttctttct ggctgacgct ggttgcgatt ctgctgatgc
     8521 cgaaactggg tctgaccgat gcgccaaatg cacagttcct tggtgtctac ctgggtctgt
     8581 ggggcgtatt tacgctgttt atgttcttcg gcacgctgaa aggcgcacgc gttctgcaat
     8641 tcgttttctt tagcctgacc gtgctgtttg ccctgctggc gatcggtaac attgccggta
     8701 acgccgcaat catccacttt gccggctgga ttggtctgat ctgcggtgcc agcgcaatct
     8761 atctggcgat gggtgaagta ctgaacgagc agtttggtcg caccgttctg ccgattggtg
     8821 aatcccacta attgtcttga gatccccccg ctcgggggga tttttttatt cgctaacgtc
     8881 gcgtcttgca ctcttcggtc tgaatgcagc aaccacttct ggtgccgttt caacgtatgg
     8941 cccttccagc aactgaatgc agtacggtac gctggcaaaa ataccgtgta ccacaacgtt
     9001 accctcagcg tccttcacac cttccagcgt ctctttaata gacttcggct gaccgggtaa
     9061 gttaaggatc agcgcctgtt tgcgaatcac acccacctga cgcgaaagga tcgcagttgg
     9121 tacaaaatgc aggctgatct ggcgcatctg ttcaccaaag ccaggcatct cgcggtccgc
     9181 tactgccagc gtcgcatcgg gcgttacgtc acgacgcgcc gggccagttc cgcccgtggt
     9241 gagcaccaga tggcaactca tttcatccac cagctcacac aacgtttgct cgatgatcgc
     9301 ctgctcatcg gggattaagc gggtttccag ttcaaacggc gtggttagcg ccgatgtcag
     9361 ccattcttcc agcgcaggga tacctttatc ctgataaacg ccgctggatg cgcgatcaga
     9421 gatggaaact aagccaatac gtaaagtatt catcaagatt tttccgttta aacagtctcg
     9481 ttaaacagaa tgatacactg cgaagggagt gacagacagg cttcagaaga ggtagcgtga
     9541 ccgacttccc ggtcacgcta agaatgatta cagcagatcg ccgatcattt tttccagttt
     9601 ttcctggtca atagcaaact tacggatacc ttccgccagt ttatctactg ccattggatc
     9661 ctggttgtgc tgccacagga actcggactc agtgatacgc gccggacgcg ctttcacttc
     9721 gccggtgtaa gacagtttac gttcgatagc cccttcgctc tcagccagct ctttcagcag
     9781 tgccggtgcg atggtcagac ggtcgcagcc tgccagttcc agaatttcgc cgatgttacg
     9841 gaagcttgcg cccataacca cggtttcata accgtgctct ttgtagtact ggtagatttc
     9901 agatacagaa accacgcccg gatcttctgc cggagcgtac tctttcttat cggtattcgc
     9961 tttgtaccag tcaagaatac ggccaacaaa cggcgagatc aggaacacgc ccgcttccgc
    10021 acaagcacga gcctgagcga aggagaacag cagggtcagg ttacagttga taccttcttt
    10081 ttccagctgt tccgcagcac ggataccctg ccaggtagaa gccagtttga tcagaatacg
    10141 atcgttgcta atacctgcat cgttgtagag tttgatcagg cgttttgctt tcgcaattga
    10201 cgcttctgtg tcataggaaa gacgcgcatc aacttcagtt gagatacggc ccggaaccag
    10261 tttcaggatt tccagaccaa tatttactgc cagtttgtca gtcgcgtcca cgatctgctg
    10321 cgcgcgatcg ttgctctgct gtttcgccca ggcgacagca tcatcaatca acttacggta
    10381 ttccggaatc tgcgctgcgt taagaatgag agaagggttg gttgtggcat cctgcggttg
    10441 atacagcttc attgccgcga tgtccccagt gtcggccact acggtggtgt actgacgaag
    10501 ggaggtcaat ttgtccgtca tgatagtatt tctctttaaa cagcttgtta gggggatgta
    10561 accggtctgc cctgatgata tcacgacgca acgtcagcgc aaccgccgac aagacggtta
    10621 tcgccaggcg agactgtttc ggatttctga cactgcgttg atatcgcccg ccatttttaa
    10681 acaaaacctc atgtatgcta cgcagaagtt atcaagtacc tcgtagcgta tatacttctt
    10741 aaacaattgg taacgtttac acaggaaagt catcgcgacc ggcaataaga gggatatgca
    10801 tgccagattt tttctccttt attaacagcg tcctttgggg atcggtaatg atttacctgc
    10861 tcttcggcgc aggttgttgg ttcacttttc gcaccggatt tgtgcagttt cgctacatcc
    10921 gccagtttgg caaaagtctt aaaaatagca ttcatccaca gccaggcggt ttaacctcat
    10981 ttcagtcatt gtgtaccagt cttgcggcgc gcgtgggtag cggcaacctg gccggcgttg
    11041 cgctggctat taccgccggt ggacctggag ccgtcttttg gatgtgggtt gccgcgttta
    11101 tcggcatggc gacctcgttt gccgaatgtt cccttgcaca actttataaa gaacgtgacg
    11161 tcaatgggca gtttcgtggc ggaccggcat ggtatatggc gcgcgggctg gggatgcgct
    11221 ggatgggcgt tctgttcgcc gtctttttgc tcatcgccta cggcataatt ttcagcggag
    11281 ttcaggcaaa cgctgttgcc cgagctctga gtttttcttt tgattttccc ccgctggtga
    11341 caggcattat tcttgctgtc tttgctctgc tggcaatcat tcgcggtctt catggcgtcg
    11401 cccggctcat gcaggggttt gtcccgttga tggcgataat ctgggtactg accagcctgg
    11461 tgatttgcgt aataaatatc gggcaacttc cccacgtcat ttggtctatt tttgagagtg
    11521 cttttggctg gcaggaagcg gcaggcggcg cggcgggata taccttaagc caggcgatta
    11581 ctaacggttt tcagcgcagt atgttttcca atgaggcggg aatggggtcg acgccaaacg
    11641 cggcagcggc agcggcgtcc tggcctccgc atccggcagc gcaaggaatt gtccagatga
    11701 ttggcatttt tatcgacacc ctggtcatct gtacggcaag cgccatgctg atattactgg
    11761 cgggtaacgg cacaacctac atgccgctgg aaggtattca gcttatccag aaggcgatgc
    11821 gggtgctaat gggttcctgg ggtgctgaat ttgttaccct cgtggttatt ctgtttgcct
    11881 tcagctccat cgttgccaac tacatttatg ccgaaaacaa tctcttcttt ttacgcctga
    11941 acaaccctaa agcgatctgg tgtttgcgga tctgcacctt cgcaacggtc atcggcggca
    12001 ccttgctaag tcttccgctg atgtggcaac tggcagatat cataatggcc tgcatggcta
    12061 ttaccaattt gactgccata ttactgctct cgcctgtggt tcataccatt gccagtgatt
    12121 atctacgcca gcgtaaactc ggcgtgcgcc cggtgtttga tccgttgcgt tatccggata
    12181 tcggtcgcca gctttctcgg gacgcgtggg atgatgtttc gcaggagtaa tcacaactat
    12241 cgatcaactc attctctttt tttgctaaag tcggcataaa tttcctgcaa ggactggata
    12301 tgctgattct tatttcacct gcgaaaacgc ttgattacca aagcccgttg accaccacgc
    12361 gatatacact gccggagctg ttagacaatt cccagcagtt gatccatgaa gcgcggaaac
    12421 tgacgcctcc gcagattagc acgctgatgc gtatcagcga caaactggca ggtatcaacg
    12481 ccgctcgctt tcatgactgg cagccagatt tcacgccgga gaatgcccgc caggcgattc
    12541 tggcgtttaa aggtgatgtc tacaccggct tgcaggccga aaccttcagc gaagacgatt
    12601 tcgattttgc ccaacagcat ttgcgaatgc tttccggctt gtatggcgta ctccgcccgc
    12661 tcgatttaat gcagccttat cgtctggaaa tggggatccg tcttgagaat gcccgaggga
    12721 aagatctgta tcaattctgg ggagatatca tcaccaacaa gctgaacgag gcgctcgcag
    12781 cacaaggcga taatgtggtg attaatctgg cgtcagatga gtattttaaa tcggtgaagc
    12841 cgaagaaatt gaatgccgag attatcaagc cggtgttcct cgatgagaag aacggcaaat
    12901 ttaagataat cagcttctac gctaagaaag cacgcggtct gatgagtcgt ttcattattg
    12961 aaaatcggct gaccaaacca gagcaactga ctggttttaa tagcgaaggt tacttctttg
    13021 atgaagcttc ctccagcaat ggcgaactgg tgtttaaacg ctacgagcag cgttaagttt
    13081 gtagtcaatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca tgcagattca
    13141 atatattgca gaattcacgt aggcctgata agacgcgcca gcgtcgcatc aggcgctgaa
    13201 tgccggattg ttacccggca tttgtcattt agcggtgatg tttgcctgga ccatgaccgc
    13261 cgtgatgatc atgatgagct ttcttatggt gacgcggcgg tggcggcggt ccgtgtgggt
    13321 gccagcgatt gcctcgccat tcataatgtt gtttccacca gccgtggtcg cgccagtgac
    13381 cgccatccca gtaataaccg cgattatcac gatcgcctat ctgtaatttt actgacggga
    13441 ctaacgtaat ttccgcagcc tgcgttgcca tgggagcgac cagaaccagg gaaagtgcga
    13501 gtacgataga ttgcatcttt ttcacaggtt actcctttta tcattgcccg ttatgggtcg
    13561 atatccggag attacgcgat gagagcggcg gttgttattc tccgcaatcc taatctctaa
    13621 gttaccgcat ttattgagaa tttctccttt ttccctgtcg tttctctcca tattttcttc
    13681 acaaaaaagc cgggctgcat aaaagcaaac ccggcctgat tgagataatg aatagatgtt
    13741 actgatgatt catcatcaat ttacgcaacg cagcaaaatc ggcgggcaga ttatgcgaaa
    13801 gcaagggtaa atcggcacgt tctgccagct cttttggcag atccaacgtt tcaccgagaa
    13861 tcgcttccac gctctcttta aatttcgccg gatgcgcggt gccgaggaac aagccatatt
    13921 cgcctggatt caactggtca cgcagcgcac gatacgctac ggcagcgtgc ggctccgaag
    13981 tgtagcccag ttcttttaac tcacgcattg tctgttgcgt ggtttcatca tccacggcgg
    14041 cataacccag ctctttcagt tgccagattt tgcggcggaa caactcttcc acacgcggcc
    14101 agttgttcgg ctgactcacg tccatcgcgt tggataacgt cgcctgagtc gctttgggtg
    14161 accactgacc gtcatgcagg aaacgtggca cggtatcgtt cacgttggtc gcagcaataa
    14221 aacgtttcac cggcagaccg agtgacttcg ccagcagacc cgccgtcaaa tcgccgaagt
    14281 ttccgcttgg caccgagaca accagctggt tgcgcgcttc ctgcggcagc tgcgcaactg
    14341 cttcaaagta gtagcaaatc tgcgccagca accggctaat gttaatcgag ttagctgagt
    14401 ttaaccccag cgccactttc agctcttcat catcaaacgc ctgcttcacc agcgcctgac
    14461 aggcatcgaa atcgccgtcg atggcaacag tttcgatatt gccgcccaat gtacagaaca
    14521 gtttttcttg cagtggactg attttgcctc gtggataaag gataaccact ttcacattcg
    14581 gtaaaccgta gaaagcatga gccactgccg ctccggtatc accggaggtc gcggtcagaa
    14641 tggtcactgg cttatcgccc gcaatatggg tcagcatttg tgccataaag cgaccgccga
    14701 aatctttaaa tgccagcgtt ggcccgtgga acaattccag acaaccgaca tcgctttcaa
    14761 cattggcgac cggagccggg aaggcaaacg ccgcgcgcac gcgctcttcc aggatttcct
    14821 gcgggatttc atcaccaata aacgccgaga ggatcttcgc actgcgggtg acaaaatcca
    14881 gcttcagcat ctcatcaatt tcagtcaggc tgaattccgg caggtcgtgc ggaaaaaaca
    14941 gcccctgatt tttgcccaac ccctgggtta cggcttgcgc aaagctgacc tgctcattgt
    15001 gatctttaag attgtagagt ttcatttagt tttccagtac tcgtgcgccc gccgtatcca
    15061 gccggcaaat atgaacaaaa ccttcctgat tttgcaggta gttcttaccc aaccagtcgg
    15121 caacgcgctg ggcggtatcc ggcttgtcac acagagcaaa caaggtcggg ccggagccgg
    15181 agataccgct cgctaccgcg ccgatttccg cgaccgcctg ccgcgcctgc cggaagccag
    15241 gcagtaaccg ttcacggtag ggttctgcga taacatcttt catcagcttc gcggcaagct
    15301 caggctgacg ggaatagcag gcgtgaatga agcctgccaa gtgtcgcccg tgcgcaatgc
    15361 aatcctggcg gcgatactgc gccggtaaaa tagcccgggc ttctgccgtc gagactttaa
    15421 tccccggata cgccagcacc cacagccact catcaaaccc tggcacttgc tggctgatga
    15481 tgtcgttttc ttcgatcatc aactgcatac caccaagaaa acacggtgcc acgttgtcgt
    15541 aatgaatgct gccggagatt cgtccttcca gctcgcccat caaagccagc aaacgggtgt
    15601 cattaagcgg cttgccgcag tgttcattca tcgccatcag agccgcgacc accgaacagg
    15661 cgctggagcc taagcccgaa ccgatcggca tattcttttc cagagtcatc gccactggaa
    15721 tttgcttgcc cagctcctgg caaaaacgct cccagcactg ataaacgata ttttcccgtg
    15781 gttctgacgg cagcttatcg gcaaagcgtc cgaggttgtt gagactgaat gtctctgccg
    15841 actcaaccgt gactacatct ccgagcaatg caccatcaac gggtgtcacc gccgccccga
    15901 gcacatcaaa cccgacgctc atattggcac tggaagccgg ggcataaact ttaaccatgt
    15961 cagactccta acttccatga gagggtacgt agcagatcgg caaagacacc ggcagcggta
    16021 acgtcattgc ccgcaccata tccgcgcagc accaacggca gcggctgata atagtggcta
    16081 taaaaggcca gggcgttttc gccatttttc actttgaaca gcggatcatt accatccact
    16141 tcggcaatct tcacgcggca gacgccatct tcatcaatat tgccaacata gcgcaaaact
    16201 tttccttcat cacgggcctt cgccacgcgc gcggcaaaga gatcgtcgag ctgtgacaga
    16261 ttcgccataa aagcggcaac atcaccctca gcgttaaact ctgcgggcag cacaggttca
    16321 atttcaatat ccgccagctc cagttcacgt cccgtttcac gagcgagaat taatagttta
    16381 cgcgctacat ccataccaga aagatcatct cgcggatccg gttcggtata acccatttcc
    16441 cgcgccagcg tagtcgcctc ggagaaactc atgccttcgt ctaacttgcc gaagatataa
    16501 gaaagcgaac ctgaaagaat gccggagaac ttcatcaatt catcaccagc attgagcaga
    16561 ttttgcaggt tctcaataac cggtaatcca gccccaacgt tggtgtcata gaggaattta
    16621 cgccgcgatt tttcagccgc atgacgcaac agatggtagt aatccatcga cgaggtgttg
    16681 gcctttttgt tcggcgtgac aacgtggaaa ccttcgcgca ggaagtcggc atattgatcc
    16741 gccactgcct ggctggaggt gcagtcaaca atgaccgggt tcagcagatg atattctttc
    16801 acgaggcgaa ttaagcgccc gagattaaac ggctctttgg cttgcgccag ttcttcctgc
    16861 cagttttcca gatttaggcc atgcacattg gtgagcagag ccttcgagtt agcaacaccg
    16921 cagacacgta agtcgatatg tttattcttc aaccagcttt gctgacgctt cagttgctcc
    16981 agcagcgcac cgccaacgcc accgacgcca atcacaaaca cttcgataac ctgatcggta
    17041 ttgaacagca tctgatgagt aacgcgcacg ccagtggtcg catcatcgtt atttaccacg
    17101 acagagattg agcgttcaga agatccctga gcaatagcga caatgttgat attggcgcgg
    17161 gccagcgcgg caaagaattt cgccgagatc ccacgcaagg tgcgcatacc atcacctacc
    17221 accgagataa tggccagccg ttccgtcacc gccagcggct ccagtaagcc ttctttcagt
    17281 tccaggtaga actcttcctg cattgcccgt tcagctcgca cacagtcgct ttgcggaacg
    17341 cagaaactga tactgtattc ggaagatgat tgcgtaatca gcaccacgga aatacgggcg
    17401 cgtgacattg cagcaaagac gcgcgccgcc atgccgacca ttcctttcat ccccgggccg
    17461 gaaacgctga acattgccat attattcaga ttggaaatgc ccttgaccgg taattcgtct
    17521 tcatcacgac tggcaccaat gagcgtacct ggagcttgag gatttccggt atttttaatc
    17581 aggcaaggga tctggaactg ggcgatgggg gtaatggtgc gggggtgaag aactttagcg
    17641 ccgaagtagg aaagctccat cgcttcctgg taggacatcg atttcaacaa cctcgcatcg
    17701 ggcacctgac gcgggtcgca ggtatatacc ccgtcaacgt ccgtccaaat ctcgcaacaa
    17761 tcggcgcgta aacaggcagc cagcaccgcc gcggagtagt cggaaccgtt gcgtccaagt
    17821 accaccagtt cgcctttttc attaccggcg gtgaaacctg ccatcagcac catgtgatca
    17881 gccggaatac gacttgccgc aatacggcgg gtggactctg caatatcgac agtagattcg
    17941 aggtaatgcc ccactgccag cagtttttcg accggatcga taacggtaac gttgtgaccg
    18001 cgcgcttcta atacgccggc cataatggcg atcgacattt tctcgccacg gcaaatcagc
    18061 gcagcgttaa tgctatccgg gcactgccct aacaaactaa tgccatgcag gacatgtttt
    18121 atttgggcaa attcctggtc gacgaaagtt ttcaattgcg ccagcgggaa tcccggctgg
    18181 gcggcggcga gtcccgtcag aagttcggca aaaatacgtt cggcatcgct gatattgggt
    18241 aaagcatcct ggccgctaat ggttttttca atcatcgcca ccaggtggtt ggtgattttg
    18301 gcgggggcag agaggacggt ggccacctgc ccctgcctgg cattgctttc cagaatatcg
    18361 gcaacccgca gaaaacgttc tgcatttgcc actgatgtac cgccgaactt caacactcgc
    18421 atggttgtta cctcgttacc tttggtcgaa aaaaaagccc gcactgtcag gtgcgggctt
    18481 ttttctgtgt ttcctgtacg cgtcagcccg caccgttacc tgtggtaatg gtaatggtga
    18541 tggtggtggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat gttgtgtact
    18601 ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt agtgacctaa
    18661 gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc tgtcagagac
    18721 tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa aagctgaaaa
    18781 atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc agcaaacggt
    18841 gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgttgtaac cagtcgacca
    18901 gttttatgtc atctgccact gccagagtcg tcagcaatgc catggctcgt tcgcgtaaag
    18961 cttgcagttg atgttggtct gccgttgtgt cacttttcgt cggttgttgt attaatattg
    19021 ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga taatccgcca
    19081 ccatcggcac accagtaaga acgtcagcca acgctaactc ttcgttagtc agcccggaat
    19141 cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc tctaacagcg
    19201 gcaccagttc tactggcgta gcgtagtaat gatatttcgc ccgactgcgc gcagtggtgg
    19261 cgacagtgaa atcgacatcg tgtaacgatt cagccagtgt cgggaaaact ttaatattat
    19321 caataatatc accagatcca tgtgcgaccc agcgggtggc tggctccagg tgtgcctgac
    19381 tatcgacaat ccgcagttcg ctaaacccca tcgttttcat tgcccgcgcc gctgccccaa
    19441 tattttctgc tctggcgggt gcgaccagaa taatcgttat acgcatattg ccactcttct
    19501 tgaccaaatg accgcgaaca gggtgatcac tgtcaactta ttacgcggtg cgaatttaca
    19561 aattcttaac gtaagtcgca gaaaaagccc tttacttagc ttaaaaaagg ctaaactatt
    19621 tcctgactgt actaacggtt taat
//
LOCUS       DAZFDC010000088        19616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFDC010000088 DAZFDC010000000
VERSION     DAZFDC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.088"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..252
                     /gene="yahH"
                     /locus_tag="ACHJ48_003233"
                     /pseudo
     CDS             1..252
                     /gene="yahH"
                     /locus_tag="ACHJ48_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YahH"
     gene            302..1252
                     /gene="yahI"
                     /locus_tag="ACHJ48_003234"
     CDS             302..1252
                     /gene="yahI"
                     /locus_tag="ACHJ48_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO4708728.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEKQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            1262..2644
                     /gene="yahJ"
                     /locus_tag="ACHJ48_003235"
     CDS             1262..2644
                     /gene="yahJ"
                     /locus_tag="ACHJ48_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO4708729.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            2943..3353
                     /locus_tag="ACHJ48_003236"
     CDS             2943..3353
                     /locus_tag="ACHJ48_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO4708730.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            3607..4590
                     /locus_tag="ACHJ48_003237"
     CDS             3607..4590
                     /locus_tag="ACHJ48_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4708731.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDVSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            4639..6123
                     /locus_tag="ACHJ48_003238"
                     /pseudo
     CDS             4639..6123
                     /locus_tag="ACHJ48_003238"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            6116..7087
                     /locus_tag="ACHJ48_003239"
     CDS             6116..7087
                     /locus_tag="ACHJ48_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4708732.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            7084..8040
                     /locus_tag="ACHJ48_003240"
     CDS             7084..8040
                     /locus_tag="ACHJ48_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO4708733.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVLPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            8127..9176
                     /gene="yahK"
                     /locus_tag="ACHJ48_003241"
     CDS             8127..9176
                     /gene="yahK"
                     /locus_tag="ACHJ48_003241"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO4708734.1"
                     /translation="MKIKAVGSYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            9419..10234
                     /gene="yahL"
                     /locus_tag="ACHJ48_003242"
     CDS             9419..10234
                     /gene="yahL"
                     /locus_tag="ACHJ48_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO4708735.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEGISNRVQELGDRYYPVNKHVKIRYTL"
     gene            10686..10895
                     /locus_tag="ACHJ48_003243"
     CDS             10686..10895
                     /locus_tag="ACHJ48_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708736.1"
                     /translation="MPLLSSLQSGTLPLFGYSGRVRPMKKAHTGESGLKWEAKDSSKL
                     IGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(10912..11583)
                     /gene="yahN"
                     /locus_tag="ACHJ48_003244"
     CDS             complement(10912..11583)
                     /gene="yahN"
                     /locus_tag="ACHJ48_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO4708737.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            11730..12005
                     /gene="yahO"
                     /locus_tag="ACHJ48_003245"
     CDS             11730..12005
                     /gene="yahO"
                     /locus_tag="ACHJ48_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO4708738.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
     gene            complement(12103..13689)
                     /gene="prpR"
                     /locus_tag="ACHJ48_003246"
     CDS             complement(12103..13689)
                     /gene="prpR"
                     /locus_tag="ACHJ48_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO4708739.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            13928..14818
                     /gene="prpB"
                     /locus_tag="ACHJ48_003247"
     CDS             13928..14818
                     /gene="prpB"
                     /locus_tag="ACHJ48_003247"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO4708740.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYHYEEKLDDLFVRSQAK"
     gene            14974..16143
                     /gene="prpC"
                     /locus_tag="ACHJ48_003248"
     CDS             14974..16143
                     /gene="prpC"
                     /locus_tag="ACHJ48_003248"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO4708741.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            16177..17628
                     /gene="prpD"
                     /locus_tag="ACHJ48_003249"
     CDS             16177..17628
                     /gene="prpD"
                     /locus_tag="ACHJ48_003249"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO4708742.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVSIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            17668..19554
                     /gene="prpE"
                     /locus_tag="ACHJ48_003250"
     CDS             17668..19554
                     /gene="prpE"
                     /locus_tag="ACHJ48_003250"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO4708743.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLNQIRQ
                     AMEE"
BASE COUNT         4761 a         4798 c         5315 g         4742 t
ORIGIN      
        1 gaacgcctta tccggcctac gaatggcgca agaatctgta ggcctgataa gcgtagcgca
       61 tcaggcattt gtcaccattg ccggatgcgg cgtgaacgcc ttatccggcc tacgaatggc
      121 acgagaattg taggcctgat aagcgtagcg catcaggcat ttgtcaccat tgccggatgc
      181 ggcgtgaacg ccttatccgg cctacgaatg gcacgagaat ttgtaggcct gataagcgta
      241 gcgcatcagg cagtctggcg ttggtcataa ccccatcacc ctctgtagcg gacataacaa
      301 catgaaagaa cttgtggtcg ttgccattgg tggcaacagc attatcaaag ataacgccag
      361 ccagtcgatt gagcatcagg cggaggcggt gaaagccgtg gccgatacgg tgctggaaat
      421 gctggcttcc gattacgaca ttgtgctgac ccacggtaac gggccgcagg tcgggctgga
      481 tttacgccgt gcggagattg cccacgagcg cgaagggctg cccttaacgc cgctggcgaa
      541 ctgtgtggca gatacgcagg gcggcattgg ctacctgatc caacaggcgc tgaacaaccg
      601 gctggcgcgt cacggcgaga aaaaagccgt caccgtggtg actcaggtgg aagtggataa
      661 aaacgatcca ggttttgccc atcccaccaa gcccattggt gcattcttca gtgaaagcca
      721 gcgtgacgaa ttacaaaagg caaaccctga ctggcgtttt gttgaagatg ctggacgggg
      781 ctatcgccgc gtggtcgcct cgccggaacc gaaacgtatt gtcgaagcac ccgccattaa
      841 ggcgctgatc caacaaggct ttgtggtgat tggcgcgggc ggcggcggaa ttcctgtggt
      901 gcgtactgac gcgggagatt accaaagcgt ggacgcggtt atcgacaaag atctctctac
      961 cgcgctgctg gcccgtgaaa ttcacgccga cattctggtg atcaccaccg gcgtcgaaaa
     1021 agtctgtatc cactttggta aacccgagaa gcaggcgctg gatcgggtgg atattgccac
     1081 catgactcgc tatatgcagg aaggacattt cccgcccggc agcatgttgc caaaaatcat
     1141 cgccagcctg acgttcctgg aacagggcgg taaagaagtg attatcacca cgccggaatg
     1201 cctgccagcg gcgctgcgcg gtgaaacggg cacccacatt attaaaacgt aaggacgtaa
     1261 gatgaaagaa aataatagcc gccgtgaatt tctgagccag agcggtaaga tggtcaccgc
     1321 cgccgcgctg tttggtacct ctgtaccgct cgcccatgcg gcggtctctg gcaccacaaa
     1381 ctgcgaagcg aacaacacca tgaaaatcac tgacccgcat tactatctcg acaacgtgct
     1441 gctggaaacc ggttttgact acgaaaatgg cgtggcggtg cagacccgca cggcgcgcca
     1501 gaccgtggag attcagaacg gcaaaattgt cgcgctgcgc gagaataagc agcacccgga
     1561 tgccacgctg ccgcactatg acgctggcga taagctgatg ctgcccacca cccgcgacat
     1621 gcatattcat ctcgacaaaa cgttttacgg cgggccgtgg cgctcgctca atcgcccggc
     1681 aggcaccacc atccaggaca tgatcaaact cgagcagaaa atgctgccgg agctgcaacc
     1741 gtacactcag gagcgggcag aaaaactgat tgatttattg cagtcgaaag gcaccaccat
     1801 tgcccgcagc cactgcaata tcgaaccggt ttccggcctg aaaaatctgc aaaatttgca
     1861 ggcggtgctg gcgcgacgtc aggcgggctt tgagtgtgaa atcgtcgcct tcccgcagca
     1921 cggtttgctg ctgtcgaaat cggaaccctt aatgcgtgaa gcaatgcagg cgggtgcgca
     1981 ttacgtcggc gggctggacc cgaccagtgt cgatggcgcg atggaaaaat ccctcgacac
     2041 catgttccag attgcgctgg actacgacaa aggcgtcgat attcacctgc acgaaaccac
     2101 tccgtcgggc gtggcagcca tcaattatat ggttgaaacg gtagagaaaa cgcctcaact
     2161 gaaaggtaag ctgaccatca gccacgcctt tgcgctggct acgctcaacg agcaacaggt
     2221 agatgaactg gcgcatcgca tggcggcgca gcaaatttct atcgcctcga cggtgccgat
     2281 tggcacgctg catatgccgc tcaaacagtt gcacgacaaa ggcgtaaaag tgatgactgg
     2341 cactgacagc gttatcgacc actggtcgcc ttatggtctg ggcgacatgc tggaaaaagc
     2401 caatctctac gcgcagctct atattcgtcc taacgaacag aacctctccc gttcgctgtt
     2461 tctagccact ggcgatgtat tgccgctgaa tgaaaaaggc gagcgtgtat ggccaaaagc
     2521 gcaggatgac gccagctttg tgctggtgga cgcctcctgt tccgccgagg cggtggcgcg
     2581 tatctcgccg agaaccgcaa cgttccataa agggcaactg gtgtggggga gtgtggcagg
     2641 ttgatgcgca tttgaaacgc ctgcaacggt gaatagtaag agatttaagc cccagggagt
     2701 ggggcgggaa tggtttgcta atgaatgatc ctgaacttta taatctcccg gcataatttc
     2761 caggggatta tttgagagaa atacacaaaa gtgagtttta acaaaggtat ttatcaggcc
     2821 ataaatgtga cctgcttcgg ataaaaaaga ttcagtatcg aaaaaccaat gatactcttg
     2881 ctgatggtta ccaacagtaa ttaagatgtg atttgttttt ccgtaatcgc aaaaatattg
     2941 ccatgaccat aaaagaaaaa ttctcacaaa aatatcccca tgcttccttt tgtacttttg
     3001 gcgattcagc cgcgttggcc gatcacctcg caacgttgat tgcgactggt gttaaaacag
     3061 cttcctgtgg ttcgctggcg ggatgtattg aagacaacgc atttccgatg attggtgagt
     3121 ataagattgt agaaaatagc cgtggtgagc ctgtatgtgt tatcagagtg attggcctgc
     3181 atttactgcg cttttctgat gtcaccgcgg agcttgcccg taaggaaggt gaaggtgacc
     3241 tcagtcttga atattggcgt aatgaacacc gtcgtttttt ccaggcagaa ggaagctatt
     3301 caccagaaat ggacgttatc tttgaagaat atgcccttat tgacgttgtg tagataacta
     3361 tttatctaca tgaaataagc tcacaaacat aaaatatcgc taattttgtt tattttgctg
     3421 ttttttacgt gatgaatgtc acacttatta ttgctgcccg ttttgtccat atttataacc
     3481 atatttttaa tagctgcctg aaatagagct gtttattctg cataaaaata ttcgaaaatt
     3541 cttattctct ccagcgcgct aatttgggcg aacacttcct gactaccctg caatgaggct
     3601 gaaatgatga ataaacgttt tgttatcaat atggtgtctt cgctgttatt aggcgctgcg
     3661 ctaatttctg cgccgctcca ggcagcggaa aaagtcgtgg tgaatatttc taaagtcgat
     3721 ggtatgccgt ggtttaatcg catgggcgaa ggtgtggttg aggcgggtaa agcctttggt
     3781 gttaatgcct cacaggtggg gccttcaagt actgatgctc cgcaacaagt taaaattatt
     3841 gaggatttaa tcgcccgtaa ggttaatgca attactatcg tgccaaacga tgccaacgtg
     3901 ctggaaccgg tattcaaaaa agcgcgagat gccggaattg tggttctgac caacgaatcc
     3961 cccggtcagc caagtgctaa ctgggatatc gaaatcattg ataacgaaaa atttgccgct
     4021 gaatatgttg agcatatggc gaaacgcatg ggtggcaagg gcggctatgt gatttacgtg
     4081 ggtagtttga ccgtgccgca gcataactta tgggctgact tgctggtgaa ataccagaaa
     4141 gagcattacc cggatatgca tgaagttacc cgccgcatgc cggttgccga gagtgtggat
     4201 gtctcacgtc gtactacact ggacctgatg aaaacctatc ctgacttgaa agcggttgtc
     4261 tcctttggtt caaacggtcc gattggcgcg ggacgtgcgg tgaaagagaa acgggcgaaa
     4321 aataaagtcg cagtctatgg catgatgatc ccttcacagg cggcatcact gattaaaagt
     4381 ggtgatatca ccgaagggat tacttacgat ccggcctctg caggttacgc actcgcagcc
     4441 gtcgccagca cgttgctgaa gggtgaagag atcaagcctg gattagaaat gcagaatctg
     4501 ggtaaggccg acgtagatat ggataagcga attatccgtt tccataaggt attactggtc
     4561 aacaaagaca atatcgactc actttattaa tccatttctt cggggcgaaa gccccgattc
     4621 ttattgccag gggatgttat ggaaaccttc ctttcccttc gtcatatcaa caaaacgttt
     4681 catgccacgc gagcgctgcg cgatgtttct ctcgacttta tgtcgggtga agtgcactgc
     4741 cttgccgggc aaaacggctg tggtaaatcg acgttgatta aaattatgtc cggtgtttat
     4801 cgcccggatg agggagcgga aattacgctt ggtgggaaaa actggtcaaa gctgacaccc
     4861 gccgcttcgg tggcgcaggg gattcaggtg atttattagg acctctcttt atttcctaac
     4921 ctgagtgtct gggagaatat cgccgtgaat cactatcacc acggtctgtt tgttaaccgc
     4981 cgtcgtctgc gtgaggtggc gcaggcggca atgactagta ttaacgtcac attgccactg
     5041 gatacgctcg tttctgagct ttcaattgcc cgctgtcagc tggtggcaat ttgccgtgca
     5101 ctggcgcagg acgcacggtt gatcgtgatg gatgaaccaa ccgcttcgct gacccatcag
     5161 gaagtgcaag ggttattaca ggtggtgcat caattgcgtg aacgcgggat ctgcgtggtc
     5221 tttgtcagtc atcgtctgga agaagtgatg gaagtttccg accgtatttc agtgctgaaa
     5281 gatggtgagc tggtcgggac ttttccggct gcagagatga ccacaaaaca actcggtttc
     5341 ctgatgaccg gccaggagtt tgaatatcag gtgcgagagt tgtggcaggg aaaatccagc
     5401 accccggtgc tggaggtgcg taatttaagt cgtcatgggg aatatctaaa tattaacttg
     5461 cgggtggaag cgggcgaagt ggtatcgatt gttggcctgc ttggtgcggg gcgcacggaa
     5521 ttatgtctga gtctgtttgg tatgacccga ccagatgctg gcgagatcct catcaacggc
     5581 cagctggtta cactgcatag taaccaggac gccatccgcc acggtattgg ttatgtttcg
     5641 gaagatcgca tgtcgcgcgg tttggtgatg gcgcaatcta ttgaagacaa catcattagc
     5701 actgtttttc acaaggtaaa agatcgcttc ggctttctga gtgaaactaa agtgcgtgac
     5761 ctggtggaca ggctaatcaa ggcattgacc atcaaagtct ctgatcctca tcttccggtg
     5821 aatacgcttt ctggtggtaa cgcgcagcga gtatcgatcg ccaaatggct ggcgattggg
     5881 ccacgactgt tgattctcga tagcccgaca gtcggtgttg atatcgccaa caaagcagga
     5941 atatacggca ttatcagcga tctggcggct catggcattg cggtattgat gatctgcgat
     6001 gaaattgaag aagcgtggta tcaaagccac cggattttgg taatgcaaaa aggtcagatc
     6061 acccatagct tcctgcctga cagcagttct caggccagga ttgcggaggt ggtaaatggc
     6121 tgaactgaaa aaacgccatg aattctggct ggcattactg attgtggtgc tgtttgttgg
     6181 ccttgcctgg cgcagcgacg agtttctgac attcggtaat ttgtacgatc tcgccaataa
     6241 ctatgccatg ttgactattc tcgcctgtgg tttgtttgtg gtgttgattt ccggtggaat
     6301 tgatatttcg tttccagcaa tgaccatcat tgcgcaatac ggcatggtgc tgttgctgca
     6361 aaaaattggt ggcaacttcg ctgtcgcgtt tgcactggca ggctgcatcg gcattttact
     6421 tggcttaatt aacgccttac tggttaatcg cctacgggtg ccttctatca tcatcactat
     6481 ctcgacgctg aatattttct atggcctgct gttatggttg agtaaaggtg tgtggctgta
     6541 cgactttccg ccgtggtttg agcagggggt tatgttgttc aagtacaccg atgctgatgg
     6601 ctatgactat ggccttggtc tgccgctgat cgccatgatt acggtggtgc tgctgacagc
     6661 gtttatcatg aatttcacca gtgtagggcg caaaatttat gcccttggcg ggaaccgcga
     6721 atcagccagt cgcatcggct ttagcgtgct gaaactgcaa cttttcgtct acggctatat
     6781 gggattgatg tctggcgctg cgggtgtagt gcagtcgtgg acggtgatga ctgtcgcccc
     6841 cgattctctt ctgggttatg agctgacagt actggctgcg gtggtgcttg gcggcactag
     6901 tttgctcggc gggcgcggca cgttaactgg tactttgctc ggcgtggtgt tgttggcagt
     6961 gatgcaaaac gggctaaatt tattgggagt ctcgtcttac tggcaaacat tgatcaccgg
     7021 catcatcatc gttgccagca ttagcgccac ggcgtggagt cagcatcaga accggagcct
     7081 gctatgaaaa aatcgtggcg taataacgtt gagttttact tgatcggcct gctggtgcta
     7141 actgttgccg cgttcagcat tactatgccg gagattttct ggtcgataag taatttccag
     7201 tccgtcgctt cgcaaatgcc cgtgctgggc attctggcgt tggctatggc ggtgaccatg
     7261 ctttgcggtg gcattaactt gtcaattata gccactgcta acgcctgttc actggtgatg
     7321 gcgtgggtgg cgactcagta tccaccgggt attgctacgg ttgtcgctac gttactggcg
     7381 ggcgctggcg cggcggtgat aatcggttta tgtaacggcg tattgattgc tggtattcga
     7441 gtgttgccaa ttcttgcaac gcttgggatg atgacgttgc tcaaagggat aaatattctg
     7501 gtgacaggtg gtagcgccat tgccaattat ccctcatggg tgctgtggtt gaatcatgcg
     7561 caatggttcg gtattccgct accgatgtgg ttgtttgccg cagtggcact ggggctgtgg
     7621 atattgctgg agaaaacgcc gctcggtaaa gccatttacc tgattgggtc taacgaacga
     7681 gcaacacttt actccggcat taacactcgt cgggtgctga tttgggttta tgtcatttcc
     7741 gccttgcttt gtgcggtagc ggcatttttg atgatgtcga aactcaactc ggctaaggcc
     7801 tcttatggcg aatcgtactt gctggtgtca atcctcgcgg cagtactcgg cggggttaat
     7861 ccggacggcg gcagcgggcg gataattggt atggtgctgg cgctgtttct tctgcaaatt
     7921 attgaaagcg gcttcaatat tctcggtata agcccctacc tgacgatggc cttatgggga
     7981 acgttgctgc tctgtttcat tcaggctcgc ggcatgttgg ggctggatcg ggtggtttaa
     8041 atattggcta tattcaatgg acgcgttttg ccgcgatgac atatcaggcg tcgccaaata
     8101 cacatagcta atcaggagta aacacaatga agatcaaagc tgttggttca tattccgcta
     8161 aacaaccact tgaaccgatg gatatcaccc ggcgtgaacc gggaccgaat gatgtcaaaa
     8221 tcgaaatcgc ttactgtggc gtctgccatt ccgatctcca ccaggtccgt tccgagtggg
     8281 cggggacggt ttacccctgc gtgccgggtc atgaaattgt ggggcgtgtg gtagccgttg
     8341 gtgatcaggt agaaaaacat gcgccgggcg atttggtcgg tgtcggctgc attgtcgaca
     8401 gttgtaaaca ttgcgaagag tgtgaagacg ggctggaaaa ctactgtgat cacatgaccg
     8461 gcacctataa ctcgccgacg ccggacgaac cgggccatac tctgggcggc tactcacaac
     8521 agatcgtcgt tcatgagcga tatgttctgc gtattcgtca cccgcaagag cagctggcgg
     8581 cggtggctcc tttgttgtgt gcagggatca ccacgtattc gccgctacgt cactggcagg
     8641 ccgggccggg taaaaaagtg ggcgtggtcg gcatcggcgg tctgggacat atggggatta
     8701 agctggccca cgcgatgggg gcacatgtgg tggcatttac cacttctgag gcaaaacgcg
     8761 aagcggcaaa agccctgggg gccgatgaag ttgttaactc acgcaatgcc gatgagatgg
     8821 cggctcatct gaagagtttc gatttcattt tgaatacagt agctgcgcca cataatctcg
     8881 acgattttac caccttgctg aagcgtgatg gcaccatgac gctggttggt gcgcctgcga
     8941 caccgcataa atcgccggaa gttttcaacc tgatcatgaa acgccgtgcg atagccggtt
     9001 ctatgattgg cggcattcca gaaactcagg agatgctcga tttttgcgcc gaacatggca
     9061 tcgtggctga tatagagatg attcgggccg atcaaattaa tgaagcctat gagcgaatgc
     9121 tgcgtggtga tgtgaaatat cgttttgtta tcgataatcg cacactaaca gactgaaaaa
     9181 attaataaat acgctgtggt ttaacatatt aacttcgctc tccacttaac tttttagtta
     9241 aggagagcga aataatatca aagtagcagt aaaacctata acgtaaattc aaattgttaa
     9301 attaactccc tccagtacct aatacttcac acgttagtta tgagcgattt ctgatagtgc
     9361 ctggtttaat cagagcttta ttatctgcga cgtttatttt tatttaagag agatcgcgat
     9421 gatatcatta aaagctccgc acaataattt gatgccatat acgcaacaaa gcatacttaa
     9481 tacggttaaa aacaatcagt taccagagga tatcaaaagc tccctggttt cctgtgtcga
     9541 tatattcaag gttttgatta aacaatatta tgattatcct tatgattgtc gtgatgattt
     9601 agtcgatgac gacaaactca ttcatctcat ggctgccgta cgagactgtg agtggtcaga
     9661 cgataacgca ctcaccataa atgtgcagtt taatgatttc cctggatttt atgactggat
     9721 ggattaccct gatcacccgg ttaagtttgt ttttcgcata cttgaaaatc aaaaagggac
     9781 agtttgggta tatgaccagg atgatgcgtt tcttgatatt aaagcaaatg tgcaggctgg
     9841 acgctttacc gggcttaaga aattagtgca gtttatcaat tcagtgcgaa cagattgcaa
     9901 atgtattctt cttgaacacc atatgccttt gctgagactt ttccctaaag ggaaagagtg
     9961 tatgcatgtg gaaaaatggc ttcgcgaaat gtcgtctatt ccggagacag acgcgcccat
    10021 taagcaggcg cttgcgcatg gattattatt acacttaaaa aatatctacc ctgtttttcc
    10081 tgaatcactg gtgatgttat tgctctctgt tctggacgta aaaacataca gagatgatgc
    10141 acgattaaat gaagggatat ctaatagagt ccaggaatta ggtgacagat attatcctgt
    10201 aaataagcac gttaaaatcc gctacactct ttagttcaga aagggttcca cctgcaagtt
    10261 gttattctac ctgagagtga atcgcaatgc aggtaatcat ttcatctgta tatctgtatg
    10321 ccaactggca ttccatctct tttttcgccg atattttgtt gtgatcggcg acacttcgct
    10381 taaaaaagca ccagtagtgg tttcgcagcc atgcggtgta taaaaaaaaa tgatctcatg
    10441 cagatgtttt gtgaatgtgt gggttgacat tcatatgaaa aaaatcataa ttccatcatg
    10501 tttgtgtggg gtcttttctg tatcttacgc atcgcactca agcctgacag aaaatatgct
    10561 gtaaggctca tatcaaaaac cgccattagc tcatcaggaa gagcagacga caaccaggat
    10621 tgttgtatgg tacggggttc gaggccccga tggcggtcca acttttcaaa actcttctga
    10681 attagatccc cttattatca tccctgcaaa gtggaacatt gccattgttt ggctattcag
    10741 gacgggtcag accaatgaaa aaagcccaca caggggagag tgggctgaaa tgggaagcta
    10801 aagactcaag taaacttatc ggaaataagg accacgcatt acgggggcta tcatcgccga
    10861 tggtcgggat tcggcaaatc aggttgatta cgtagagagt aaattatctg ctcaccgctg
    10921 cgtcacccct tcgtaaatca ggcgtagcgc gaatacacca ataattgcac caataacccg
    10981 actggcaacg cgttgcatac gcccataagc acgacgcaca gcgggcaaag aaaacgcctg
    11041 actaagaaaa actcgccaga taattgatgc gagcacaatc cccgcccagg ccattaaacg
    11101 tgcccatgtt ggtgtttcgg catttaatgt tactgagaaa atactaataa aaaataaaac
    11161 ggtttgtggg ttagagagat cggtaattaa tccacggcga aaaaagacat accagggggc
    11221 gttaatcggt tgttgtagtg tgctcatttg cggtgttgac tggcggcgca tgctgcacca
    11281 cgcaaaccat aagagataag cgccgccgac gattctgata agcgaaaaaa tctcctcaca
    11341 ctgcgtaatt agcgttgcaa gaccaaacaa acccaacccg gaataaaatg catcgcccag
    11401 cgccacgccc agtccggtca gcactcctgc gcgtcgaccg gaagccaggc tggtttgtac
    11461 taccacaaaa agattggctc ccggattaaa aaaagtaatc acgaacagtc ctacggtcag
    11521 gtaaacggca tgcaaaggat ccatagtgat ttcatccata aataagtgaa ctaactgcat
    11581 catatttcta ccaaaaataa tcgggtgcga gagagatcac aaagtgtctt atttccggtt
    11641 actggcgttt atgccctgac tgaactaatt attaatcaac ccaataatgt cggtgggtga
    11701 tagtgtgata acaactctgg agccgtaata tgaaaataat ctctaaaatg ttagtcggtg
    11761 cgttagcgtt tgccgttacc aatgtctatg ccgctgaatt gatgaccaaa gcggaatttg
    11821 aaaaagttga atcgcagtat gaaaaaatag gtgatatttc aaccagtaat gaaatgtcga
    11881 ctgcagatgc aaaagaagat ttaatcaaaa aagccgatga aaaaggggct gatgtgttgg
    11941 tactgacctc cggtcaaact gacaataaga tccacggcac ggcggatatt tataagaaga
    12001 agtaattctg aatcctatgt aaacatcccc gatgcgtaag tttatcggtg atcatctatt
    12061 gaaatttatg ccggataaag cgttcgcgct gcattcggca gttcagcttt tcagccgccg
    12121 ccagaacgtc gtccggctga tgcctaaata attcgccgct gctgttttat cgccattaaa
    12181 tttctccagt gcctgttgtg gtgtcagcaa gcgtggagcg ggaattttcg ccgactcgcg
    12241 cgccagttcc ggcagtagca gctgcaaaaa ctgcggcgtt aaatccggcg tcggttccac
    12301 acttaaaaat agcgccagtc gctccatcat attgcgcagt tcacgaatat tacctggcca
    12361 gtcgtagtgc accagcacgg tttcgcttgc ctgtaatccc tggcgtaatg cggcagaaaa
    12421 tggggcggag agcgccgcca gagacacttt caaaaagctt tccgccagcg gaagaatatc
    12481 tgccacccgc tcgcgcagtg gtggcaattg cagacgcaaa atactcagcc gataaaacag
    12541 atcacggcga aactgccctt gccgcatatc ttcttccaga ttgcagtgag tggcgctaat
    12601 gacccgtaca tctaccggaa caggctgatg cccgccgacg cgggtgacct ctttttcttc
    12661 cagcacccgt aacaggcgag tctgcaaagg tagcggcatt tcgccaatct catccagaaa
    12721 cagcgtgcca ccgtgggcaa tttcgaacag cccggcgcga cctccgcgtc gcgagccggt
    12781 aaacgcccct tcctcatagc caaacagctc cgcttccagc agcgattcgg caatcgcccc
    12841 gcagttgacg gcaacaaacg gatgcgactt tttgccctgt cgcgcatcgt ggcgggcaaa
    12901 atattcccga tgaattgcct gggccgccag ctctttgccc gtccccgttt ccccctcaat
    12961 caacaccgcc gcactggagc gggcatacag caaaatagtc tgccgcacct gctccatctg
    13021 tggtgattga ccgagcatat cgcccagcac gtaacgagta cgcagggcgt tgcgggtggc
    13081 atcgtgagtg ttatggcgta acgacatgcg cgtcatatcc agcgcatcgc tgaacgcctg
    13141 gcgcacggtg gcggcggaat agataaaaat tccggtcatt ccggcttctt ctgccagatc
    13201 ggtaatcagc cccgcgccga ccaccgcttc ggtgccgtta gcttttagct cgttaatctg
    13261 cccgcgtgcg tcttcttcgg taatgtagct acgttgatcg aggcgcaaat taaaggtttt
    13321 ttgaaacgcc accagtgccg gaatagtttc ctgataagtg acaacgccga tagaagaggt
    13381 gagttttccg gcttttgcca gtgcctgtaa cacatcgtag ccgctcggtt taatcaaaat
    13441 aactggcact gacaggcggc ttttcaggta cgcgccgtta gagccagcgg cgatgatggc
    13501 gtcacagcgt tcgtttgcca gtttcttgcg gatgtaggtc actgcttttt caaagccaag
    13561 ctgaataggg gtaatgttcg ccaggtgatc aaactcgagg ctgatatcgc gaaacagctc
    13621 gaataagcgc gttacagata ccgtccagat aaccggtttg tcgtcattca gccgtggtgg
    13681 atgtgccata gcgcaccgca aagttaagat accgaatatt gggtttagtc ttgtttcata
    13741 attgttgcaa tgaaacgcgg tgaaacattg cctgaaacgt taactgaaac gcatatttgc
    13801 ggattagttc atgactttat ctctaacaaa ttgaaattaa agatttaatt ttattaaggc
    13861 aattgtggca caccccttgc tttgtcgtta tcaacgaaaa taacaagttg ataacaaagg
    13921 atgggctatg tctctacact ctccaggtaa agcgtttcgc gctgcactga ctaaagaaaa
    13981 tccattgcag attgttggca ccattaacgc caaccatgcg ctgttggcgc agcgtgccgg
    14041 atatcaggca atttatctct ccggcggtgg cgtggcggca gggtcgctgg ggctgcccga
    14101 tctcggtatt tctactcttg atgacgtgtt gacagatatt cgccgtatca ccgacgtttg
    14161 ttcgctgccg ctgctggtgg atgcggatat cggttttggt tcttctgctt ttaacgttgc
    14221 gcgtacggtg aaatcgatga ttaaagccgg tgcggcggga ttgcatattg aagatcaggt
    14281 tggtgcaaaa cgctgtggtc atcgcccgaa caaagcgatc gtctcgaaag aggagatggt
    14341 ggatcggatc cgcgcggcgg tggatgcgaa aaccgatcct gattttgtga tcatggcgcg
    14401 caccgatgcg ctggcggtag aggggctgga tgcggcgatc gagcgtgcgc aggcctatgt
    14461 tgaagcgggt gccgagatgt tgttcccgga ggcgattacc gaactcgcca tgtaccgcca
    14521 gtttgccgat gcggtgcagg tgccaatcct cgccaacatt accgaatttg gcgccacgcc
    14581 gctgtttacc accgacgaat tacgcagcgc tcatgtcgcg atggcgctgt acccgctttc
    14641 agcgtttcgc gccatgaacc gcgccgccga acatgtctac aacgtcctgc gccaggaagg
    14701 cacgcagaaa agcgtcatcg acaccatgca gacccgcaac gagctgtacg aaagcatcaa
    14761 ctactaccac tacgaagaga agctcgacga cctgtttgtc cgtagccagg cgaaataaaa
    14821 aacgcccgtt ggttgtattc gacaaccgat gcctgatgcg ccgctgacgc gacttatcag
    14881 gcctacgggg tgaactgtag gtcggataag acgcatggcg tcgcatccga caacaatctc
    14941 gaccctacaa atgataacaa tgacgaggac aatatgagcg acacaacgat cctgcaaaac
    15001 agtacccatg tcattaaacc gaaaaaatct gtggcgcttt ctggcgttcc ggcgggcaat
    15061 acggcgctct gcaccgtggg taaaagtggc aatgacttgc attaccgtgg ctacgatatt
    15121 cttgatctgg cggaacattg cgaatttgaa gaagtggcgc atctgctgat ccacggcaaa
    15181 ctgccgaccc gtgacgaact cgccgcctac aaaacgaaac tgaaagccct gcgcggttta
    15241 ccggctaacg tgcgtaccgt gctggaagcc ttaccggcgg cgtcacaccc gatggatgtt
    15301 atgcgcaccg gcgtttccgc gctcggctgc acgctgccag aaaaagaggg gcataccgtc
    15361 tctggcgcgc gggatattgc cgacaaactg ctggcgtcac ttagctcgat tcttctctac
    15421 tggtatcact acagccacaa cggcgaacgc atccagccgg aaaccgatga cgactccatc
    15481 ggtggtcact tcctgcatct gctgcacggc gaaaagccgt cgcaaagctg ggaaaaggcg
    15541 atgcatattt cgctggtgct gtacgccgaa cacgagttta acgcctccac ctttaccagc
    15601 cgggtgattg cgggcactgg ctctgatatg tattccgcga ttattggcgc gattggtgca
    15661 ctgcgcgggc cgaagcacgg cggggcgaat gaagtgtcgc tggagatcca gcaacgctac
    15721 gaaacgccgg acgaagccga agccgatatc cgcaagcggg tggaaagcaa agaagtggtc
    15781 attggttttg gtcatccggt ttataccatc gccgacccgc gccaccaggt gattaaacgt
    15841 gtggcgaagc agctctcgca ggaaggcggc tcgctgaaga tgtacaacat cgccgatcgc
    15901 ctggaaacgg tgatgtggga gagcaaaaag atgttcccca atctcgactg gttctccgct
    15961 gtttcctaca acatgatggg cgttcccacc gagatgttca caccactgtt tgttatcgcc
    16021 cgcgtcaccg gctgggcggc gcacattatc gaacaacgtc aggacaacaa aattatccgt
    16081 ccttccgcca attatgttgg accggaagac cgccagtttg tcgcgctgga taagcgccag
    16141 taaacctcta cgaataacaa taaggaaacg tacccaatgt cagctcaaat caacaacatc
    16201 cgcccggaat ttgatcgtga aatcgttgat atcgtcgatt acgtcatgaa ctacgaaatc
    16261 agctccaaag tagcctacga caccgcacat tactgtctgc tcgacacgct cggctgcggt
    16321 cttgaagctc tcgaataccc agcctgtaaa aaactgctgg ggccaattgt ccccggcacc
    16381 gtcgtaccta acggcgtgcg cgtccccgga actcagttcc agctcgaccc cgtccaggcg
    16441 gcatttaaca tcggcgcgat gatccgctgg ctcgatttca acgatacctg gcttgcggca
    16501 gagtggggcc atccttccga caatctcggc ggcattctgg caacggcgga ctggctttca
    16561 cgcaacgcgg tcgccagcgg caaagcgccg ttgaccatga aacaggtgct gaccggaatg
    16621 attaaagccc atgaaattca gggctgcatc gcgctggaaa actcctttaa ccgcgtcggc
    16681 ctcgaccacg ttctgttagt gaaagtggct tccaccgccg tggtcgccga aatgcttggc
    16741 ctgacccgcg aggaaattct caacgctgtt tcgctggcgt gggtggacgg tcagtcgctg
    16801 cgcacctatc gccatgcgcc gaacaccggc acgcgtaaat cctgggcggc gggcgatgcc
    16861 acttcccgcg cggtacgtct ggcactgatg gcgaaaacgg gcgaaatggg ttacccgtca
    16921 gccctgactg cgcctgtgtg gggcttctac gacgtctcct ttaaaggtga atcgttccgc
    16981 ttccagcgtc cgtacggttc ttacgtgatg gaaaatgtgc tgttcaaaat ctccttcccg
    17041 gcggagttcc actcccagac ggcagttgaa gcagcgatga cgctctatga acagatgcag
    17101 gcagcaggca aaacggcggc agatatcgaa aaagtgtcca tccgcaccca cgaagcctgt
    17161 attcgcatca tcgacaaaaa ggggccgctc aataacccgg cagaccgcga ccactgcatt
    17221 cagtacatgg tggcgatccc actgctattc gggcgcttaa cggcggcaga ttacgaggac
    17281 aacgttgcgc aagataaacg cattgacgcc ctgcgcgaga agatcaattg ctttgaagat
    17341 ccggtattta ccgctgacta ccacgacccg gaaaaacgcg ccatcgccaa tgccattacc
    17401 cttgagttca ccgacggcac acgatttgaa gaagtggtgg tggagtaccc cattggtcat
    17461 gctcgccgcc gtcaggatgg tattccgaaa ctggtcgata aattcaaaat caatctcgcg
    17521 cgccagttcc cgactcgcca acagcagcgc attctggagg tttctctcga cagagctcgc
    17581 ctggaacaga tgccggtcaa tgagtatctc gacctgtacg tcatttaagt aaacggcggt
    17641 aaggcgtaag ttcaacagga gagcattatg tcttttagcg aattttatca gcgttcgatt
    17701 aacgaaccgg agcagttctg ggccgagcag gcccggcgta ttgactggca gacgcccttt
    17761 acgcaaacgc tcgatcacag caatccgccg tttgcccgtt ggttttgtga aggccgaacc
    17821 aacttgtgcc acaacgctat cgaccgctgg ctggagaaac aaccagaggc gctggcgctg
    17881 attgccgtct cttcggaaac agaagaagag cgcaccttta cctttcgcca gcttcatgac
    17941 gaagtaaacg cggtggcgtc aatgctgcgc tcgctgggcg tgcagcgtgg cgatcgggtg
    18001 ctggtgtata tgccgatgat tgccgaagcg catattactc tgctggcctg cgcgcgcatt
    18061 ggcgctattc actcggtggt gtttggggga tttgcctcgc acagcgtggc ggcgcgaatt
    18121 gatgatgcta aaccggtgct gattgtctcg gctgatgccg gagcgcgcgg tggcaaaatc
    18181 attccctata aaaaattgct cgacgatgcg ataagtcagg cgcagcacca gccacgccat
    18241 gttttgctgg tggatcgcgg gctggcgaaa atggcacgcg tcagcgggcg ggatgtcgat
    18301 ttcgcgtcgt tgcgccatca acacatcggc gcgcgggtac cggtggcgtg gctggaatcc
    18361 aacgaaacct cctgcattct ctacacctcc ggcacgaccg gcaaacctaa aggcgtgcag
    18421 cgtgacgttg gcggatatgc ggtggcgctg gcgacctcga tggacaccat ttttggcggc
    18481 aaagcgggcg gtgtgttctt ttgtgcttcg gatatcggct gggtggtggg gcattcgtat
    18541 atcgtctacg cgccgctgct ggcggggatg gcgactatcg tttacgaagg attaccgacc
    18601 tggccggact gcggcgtgtg gtggaaaatc gtcgagaaat atcaggttag ccggatgttc
    18661 tcagcgccga ccgccattcg cgtgctgaaa aaattcccta ccgctgaaat tcgcaaacac
    18721 gatctctcgt cgctggaagt gctctatctg gctggagaac cgctggacga gccgaccgcc
    18781 agttgggtga gcaatacgct ggatgtgccg gtcatcgaca actactggca gaccgaatcc
    18841 ggctggccga ttatggcgat tgctcgcggt ctggacgaca ggccgacgcg tctgggaagc
    18901 cccggcgtgc cgatgtatgg ctataacgtg cagttgctta atgaagtcac cggcgaaccg
    18961 tgtggcgtca acgagaaagg gatgctggtg gtggaagggc cgctgccgcc ggggtgtatt
    19021 cagaccatct ggggcgacga cgaccgcttt gtgaagacgt actggtcgct gttttcccgc
    19081 ccggtgtacg ccacctttga ctggggcatc cgtgacgctg acggttatca ctttattctc
    19141 gggcgcactg acgatgtgat taacgttgcc gggcatcggc tggggacgcg cgagattgaa
    19201 gagagtatct ccagtcatcc gggcgttgcc gaagtggcgg tggttggggt gaaagatgcg
    19261 ctgaaagggc aggtggcggt ggcgtttgtc attccgaaag agagcgacag tctggaagat
    19321 cgtgatgtgg cgcattcgca agagaaggcg attatggcgc tggtggacag ccagattggc
    19381 aactttggcc gcccggcgca cgtctggttt gtctcgcaat tgccaaaaac gcgatccgga
    19441 aaaatgctgc gccgcacgat ccaggcgatt tgcgaaggac gcgatcctgg ggatctgacg
    19501 accattgatg atcctgcgtc gttgaatcag atccgccagg cgatggaaga gtaaggtatt
    19561 gtcggatgcg tcgcgtggcg catccgacag ttgtgcgcag atgcctgatg cgacgc
//
LOCUS       DAZFDC010000089        19454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFDC010000089 DAZFDC010000000
VERSION     DAZFDC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.089"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(126..502)
                     /gene="rnpB"
                     /locus_tag="ACHJ48_003251"
     ncRNA           complement(126..502)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHJ48_003251"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(535..1680)
                     /gene="garK"
                     /locus_tag="ACHJ48_003252"
     CDS             complement(535..1680)
                     /gene="garK"
                     /locus_tag="ACHJ48_003252"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO4708744.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1777..2667)
                     /gene="garR"
                     /locus_tag="ACHJ48_003253"
     CDS             complement(1777..2667)
                     /gene="garR"
                     /locus_tag="ACHJ48_003253"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO4708745.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2697..3467)
                     /gene="garL"
                     /locus_tag="ACHJ48_003254"
     CDS             complement(2697..3467)
                     /gene="garL"
                     /locus_tag="ACHJ48_003254"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO4708746.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3483..4817)
                     /gene="garP"
                     /locus_tag="ACHJ48_003255"
     CDS             complement(3483..4817)
                     /gene="garP"
                     /locus_tag="ACHJ48_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO4708747.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5192..6763
                     /gene="garD"
                     /locus_tag="ACHJ48_003256"
     CDS             5192..6763
                     /gene="garD"
                     /locus_tag="ACHJ48_003256"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO4708748.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPKAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6912..7247
                     /gene="prlF"
                     /locus_tag="ACHJ48_003257"
     CDS             6912..7247
                     /gene="prlF"
                     /locus_tag="ACHJ48_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO4708749.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7247..7711
                     /gene="yhaV"
                     /locus_tag="ACHJ48_003258"
     CDS             7247..7711
                     /gene="yhaV"
                     /locus_tag="ACHJ48_003258"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO4708750.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(7766..8575)
                     /gene="agaR"
                     /locus_tag="ACHJ48_003259"
     CDS             complement(7766..8575)
                     /gene="agaR"
                     /locus_tag="ACHJ48_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO4708751.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8824..10104
                     /gene="kbaZ"
                     /locus_tag="ACHJ48_003260"
     CDS             8824..10104
                     /gene="kbaZ"
                     /locus_tag="ACHJ48_003260"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO4708752.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLNRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10127..10600
                     /gene="agaV"
                     /locus_tag="ACHJ48_003261"
     CDS             10127..10600
                     /gene="agaV"
                     /locus_tag="ACHJ48_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO4708753.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGIPTEPAVDLFKLL"
     gene            10611..11390
                     /gene="agaW"
                     /locus_tag="ACHJ48_003262"
     CDS             10611..11390
                     /gene="agaW"
                     /locus_tag="ACHJ48_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO4708754.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11380..12258
                     /gene="agaE"
                     /locus_tag="ACHJ48_003263"
     CDS             11380..12258
                     /gene="agaE"
                     /locus_tag="ACHJ48_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO4708755.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12276..12710
                     /gene="agaF"
                     /locus_tag="ACHJ48_003264"
     CDS             12276..12710
                     /gene="agaF"
                     /locus_tag="ACHJ48_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO4708756.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12707..13840
                     /gene="nagA"
                     /locus_tag="ACHJ48_003265"
     CDS             12707..13840
                     /gene="nagA"
                     /locus_tag="ACHJ48_003265"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO4708757.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVSVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLSTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14191..15345
                     /gene="agaS"
                     /locus_tag="ACHJ48_003266"
     CDS             14191..15345
                     /gene="agaS"
                     /locus_tag="ACHJ48_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO4708758.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALHSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELHRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15358..16218
                     /gene="kbaY"
                     /locus_tag="ACHJ48_003267"
     CDS             15358..16218
                     /gene="kbaY"
                     /locus_tag="ACHJ48_003267"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO4708759.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16385..16861
                     /gene="agaB"
                     /locus_tag="ACHJ48_003268"
     CDS             16385..16861
                     /gene="agaB"
                     /locus_tag="ACHJ48_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO4708760.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            16933..17703
                     /gene="agaC"
                     /locus_tag="ACHJ48_003269"
     CDS             16933..17703
                     /gene="agaC"
                     /locus_tag="ACHJ48_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO4708761.1"
                     /translation="MAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDIQTGLITGG
                     LTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSLLMQYVILF
                     FYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLAQGAMQALV
                     KAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQVSNLLPVA
                     VLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17693..18484
                     /gene="agaD"
                     /locus_tag="ACHJ48_003270"
     CDS             17693..18484
                     /gene="agaD"
                     /locus_tag="ACHJ48_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO4708762.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18485..19240
                     /gene="agaI"
                     /locus_tag="ACHJ48_003271"
                     /pseudo
     CDS             18485..19240
                     /gene="agaI"
                     /locus_tag="ACHJ48_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
BASE COUNT         4703 a         5059 c         4957 g         4735 t
ORIGIN      
        1 tttacgtaaa tcaggtgaaa ctgaccgata agccgctttc ttttgggtat agtgtcgtgg
       61 acagtcattc atctttctgc ccctccaaaa gcaaaaaccc gccgaagcgg gtttttacgt
      121 aaatcaggtg aaactgaccg ataagccggg ttctgtcgtg gacagtcatt catctaggcc
      181 agcaatcgct cactggctca agcagcctac ccgggttcag tacgggccgt accttatgaa
      241 cccctatttg gccttgctcc gggtggagtt taccgtgcca cggactgtta ccagccgcgc
      301 ggtgcgctct taccgcaccc tttcaccctt acctgatccc gcttgcgcgg gccatcggcg
      361 gtttgctctc tgttgcactg gtcgtgggtt tcccccccag gcgttacctg gcaccctgcc
      421 ctatggagcc cggactttcc tcccctccgc ccgtctcccc cgaagaggac gacgacgaag
      481 cggcgactgt ctggtcagct tcggcgcgca gtatagaggg tttgcgcgcc cttgtcaccc
      541 cgcgttgcgc attccaatcg ccagtgtcgc ggcgatatta cgtgaagcac ggcagatatt
      601 gtcataagcc ccgcggaatg cttcgtccaa cgtacctatg ctggtcaata cgctgaagac
      661 cgcatcaatg ccatgctgat gtacaacgcc aacatcatcg gtcaggctac ccgcaatgcc
      721 aatcaccggt ttatggtact tcttcgccac gtttgcgaca ccaatcggta ccttgccgtg
      781 aatactctgg ctgtcaatac gcccttcacc ggtgatcacc agcgtacaat cgtgaatatg
      841 ttcctccaga ttcagcgccg tagtgacgat ttcaatacca cttttcagtt ccgcaccaag
      901 aaacgccatt agcgccgcgc ccataccacc cgcagctcct gcgccgggga catctttcac
      961 atcaacatgc agcgcttttt taatgacctc ggcatagtga gagaggttat tgtccagctc
     1021 aacaatcatc gcttcactgg ctcccttttg tgggccaaag atgcgcgatg cgccgttatc
     1081 gcccaccagc ggattggtga catcacaagc gacgcgaatg acgcaatctt ttaagcgcgg
     1141 atcgaggccg gaaatatcaa tatcattcag agtattaaga ctaccgccgc caaaaccaat
     1201 ttcattgccg ttggcgtcgc ataatttcgc ccccagcgcc tgtaccatgc ctgcgccgcc
     1261 atcatttgta gcgctgccgc caatgccgat aataatgttt gtcgcgccgc tctccagcgc
     1321 ctgcaggatt aactcgcctg tgccgcgtga agtggtcacg agtggatcgc gtttttccgc
     1381 aggtaccagc tccagcccac tggccgccgc catttcaata aacgcggttt tgccatcgcc
     1441 ggagatcccc caactggcat tcactttctc gcccagcggc cctgtaaccc aggcgtgacg
     1501 ttcggaaccc tgggtggctg caatcatcgc ttccaccgtt ccttcgccac cgtcggcaac
     1561 aggaacagaa acgtactgtg catcaggaaa aatttcccga aatccttttt ctatcgcctg
     1621 cgcaacctcg ctggcagata aactttcttt ataagagtct ggggcgatta cgattttcat
     1681 acctatgcct gttaccacat gacgccggag ggcgtttctc ttattcggcc tggattccag
     1741 gcccggattg caatacgcca tccgggcacg acgtcattaa cgagtaactt cgactttcgc
     1801 cagtttttcg tagtagcacg ccagggcgct atgatccgcc gttcctaaac catctgcacg
     1861 cagtgcctgc atcatctcca taaccgcagc tgtgagcggc agttgtgcgc cgacgccgtg
     1921 agaagtatcc agcgcattcg ccagatcctt aatatgcaga tcaatacgga agcccggctt
     1981 gaagttgcgg tccatcacca tcggcgcttt ggcatccagc acggtactgc ccgccagtcc
     2041 accgcgaatt gcctgataaa ccaggtccgg gttaacgccc gctttagttg ccagcgttaa
     2101 cgcttctgac atcgcggcaa tattcagcgc cacaatgacc tgatttgcca gtttggtgac
     2161 gttacctgca ccgatttccc cggtatgcac cacggaaccc gccatcgctt tcatcaaatc
     2221 atagtatttg tcgaaaatag ccttgtcgcc gcctaccatc actgacagcg taccgtcgat
     2281 ggctttcggt tcaccgccgc tcaccggagc atccagcata tcaatgcctt tcgctttcag
     2341 cgcttcgctg atttcacggc ttgccagcgg tgcgatagaa ctcatatcga tcaataccgt
     2401 acctggcttc gcgccttcaa taatgccatt ctcacccagc gccacctctt tcacatgagg
     2461 ggagtttggc agcatggtta tgatgacgtc gcactgttca gcgatcgctt tagccgtaga
     2521 agctgtttct gcacctgcag caatcacgtc agcaatagct tctgggttac ggtcagcaac
     2581 caccagcgag taacctgctt tcagaaggtt tttactcatt ggtttaccca taatccccag
     2641 gccaataaaa ccaactttca tagtcatatc aatcatctct cttgttgcgg tggtggttat
     2701 tttttaaagg tatcagccag tttctgagtg gcagagcgga agacgccgag atcgctgccg
     2761 acagccacaa acgtcgcgcc ccattccaga taacgacgcg catcggcttc gaccggcgcg
     2821 aggataccgc tgggtttgcc gtgcgcgctg gcacggttaa aaatgtgctg aattgctttt
     2881 tgtacatccg ggtgtgatgc attgccgaga tggcctaatg ccgcggccag atcgctgggg
     2941 ccgacgaaga tgccgtctac gccttcggta gcggcaatag catcgacgtt atctacgccc
     3001 tgctgacttt ctatctggac cagaatagtg atgttcttgt tcgactgagc gaaataatcc
     3061 gccacggtgc caaacatatt ggcgcggtga gaaacggaga cgccgcgaat gccttctggc
     3121 ggataacgcg tcgatgccac cgcctgctct gcttcctctt ttgtttctac aaaaggaatc
     3181 aggaagttat agaaaccgat atccagaaga cgcttaataa ttaccggctc gttggtcggc
     3241 actcgcacta ctggcgcgct ggcgctgcct ttcagggcca ttaactgcgg aataaacgtt
     3301 gaaatatcgt ttggcgcatg ttcgccatcc agcaccagcc agtcaaaccc agccaaacca
     3361 agaacttcag tgctaatcgg gttagagagt gctgaccagc aaccaatttg tacctgtttc
     3421 gcagccagtg cggctttgaa tttattcggg aaaacatcgt tattcatcgc ttataccttt
     3481 gcttatttct gcaattccat acgtttaatg tcgccaacta cgaagaggta gcagaccatc
     3541 gccattagcg ctgaacatcc cacgaaaacc agtgctgcat tgaaggagtg cagttcactt
     3601 accaggtagc caatcaccag tggagtgaca atggaggcaa cattgccaaa gacgttaaag
     3661 acgccgccgc agaggccaac aatctcttta ggcgcggtgt cagaaatcac cggccagccc
     3721 agcgcaccaa atcctttacc aaagaaagcc agcgccatca gcatgaccac cagcgtggtg
     3781 ttgttggtgt agttacataa gatgatggtg gaagccagca acattcccag cacaatcggt
     3841 agcttacgtg ccagggtcag ggataaaccg cgtttgatca gataatccga gaagacacct
     3901 cccagcacgc cgcccgcaaa accacacagt gctggaatcg aggcgaccag acccactttc
     3961 agaatcgaca tacctttttc ctgcaccaga taaatcggga accaggtgag gaagaaccag
     4021 gtgatggtgt tgataaaata ttgtccgaaa aatacgccca acatcatgcg gttagagagc
     4081 aattgcttga tgtaatgcag tttgggtccg cttgctgccg cactgcccgg ctttttgtgg
     4141 tccatatcga ccaccgcgcc attttcagag ataaacttca gctcttccgc agacatacgt
     4201 gggtgatctg tcgggttatg aatcaatttg atccacagcg ccgtcagcac aaaaccaatc
     4261 acccccataa cggtaaagac gtgctcccag ccccaggcga aagtcagcca gccaagcaga
     4321 ggcgaaaaga gcgccagcga gaaatattgc gccgagttaa agatggcgga ggcagtacca
     4381 cgttctttcg tcgggaacca ggcggcgaca atacgggcgt tcgccgggaa tgatggcgct
     4441 tccgagaagc cgagcataaa gcgcataaag aacatggaga tccctgccca ggccagcggg
     4501 aacatatcaa caaagccttg caggaaggtg aatagcgacc agaaaaagag gctgtaggtg
     4561 taaacttttt tcgagccaaa cttatcaagc agccagccgc cggggatttg catcagcaag
     4621 taggcccagc caaaagcaga aaagatgtaa cccatcgaaa ccgcacttaa ctgcaactct
     4681 tttgccactt cggtaccagc aatagacagc gttgcacgat cggcgtagtt aacggcggta
     4741 acaataaaaa taatcagtaa tattaaatag cgggtatgca cgcctttctt tttttcgtca
     4801 acggtgtcca gaatcatttt atttacctcg ggtacttatg ctgattttta ttattatggg
     4861 gaagatgtta tttatgagtt tcatttatgc cgtaacgaca atgaactcgg gaattagtat
     4921 aagcagcgcg agaataataa tcattgtgca aatgctaatt taattaatac tatttaaata
     4981 ttattttgag catatgcaca taaggttgcg cgctaaagca cagatttgcg ctttaccttg
     5041 ccgggcggca ttgcaatccc tgaaatgatt gacattgatc acatttctgc gtttaaactc
     5101 ctgacattct tatttcaccc aatgaagtca tttattttta aatgagacca ggtcctcatt
     5161 ttaataacct ctggctggag aatattgcac aatggccaac atcgaaatca gacaagaaac
     5221 gccaactgcg ttttatataa aagttcacga cacagataat gtggcaatta ttgttaatga
     5281 taatggcctg aaagcaggaa cgcgttttcc ggatgggctg gaattaattg aacatattcc
     5341 ccaggggcat aaagtcgcat tgctcgacat tccggctaat ggtgaaatta ttcgttatgg
     5401 cgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga agctggatcg acgaatcaat
     5461 ggttgtactg ccgaaagcgc cgccgttaca cacgctgcca ctggcaacca aagtcccgga
     5521 acccttaccg ccgctggaag gatacacctt tgagggctat cgcaatgccg atggcagcgt
     5581 gggcaccaaa aacctgctcg gcatcaccac cagcgtccac tgtgtggcag gcgtggtgga
     5641 ctacgtagta aaaatcattg aacgcgatct gctaccgaaa tacccgaacg tcgatggcgt
     5701 ggtggggctg aatcatttgt acggttgtgg cgtggcgatt aacgcaccgg cggcagttgt
     5761 acctatccgt accattcaca atatttcgct gaatcctaac tttggcggcg aagtaatggt
     5821 gattggcctg ggttgtgaaa agttgcagcc tgagcgcctg ctgactggaa cggatgatgt
     5881 gcaagctatt ccagtagaaa gcgccagcat tgtcagtttg caggatgaaa agcatgtcgg
     5941 ttttcagtcc atggtcgagg atattttgca ggtcgccgaa cgccatctac aaaaactgaa
     6001 tcaacgtcag cgagaaacct gtccggcttc agaactggtt gtcggcatgc agtgcggtgg
     6061 cagcgatgcc ttttctggcg taacggcaaa cccggcggtt gggtatgcgt ctgatctact
     6121 ggtgcgctgc ggcgcaacgg tgatgttctc agaagtcacg gaagtgcgtg acgcgatcca
     6181 tctgctgaca ccacgcgcag tgaacgaaga ggtcggcaaa cggctgctgg aggagatgga
     6241 gtggtacgat aactatctca atatgggaaa aaccgaccgc agcgccaacc cttcgccggg
     6301 caacaagaaa ggcggtctgg caaatgtagt ggagaaagcg ctcggctcca ttgctaaatc
     6361 gggtaaaagt gcaattgttg aagtgctgtc gcccggtcaa cgcccgacta aacgcggatt
     6421 aatttacgcc gcgacgccag ccagcgattt tgtctgtggc acgcaacagg tggcttcggg
     6481 tatcaccgtg caagtgttta cgaccggtcg tggtacgccg tacggcctga tggcggtacc
     6541 cgtcattaaa atggcgaccc gcaccgagct ggcgaaccgc tggtttgatt taatggatat
     6601 taacgcgggc actatcgcca ccggcgaaga aaccattgaa gaggtgggct ggaagttgtt
     6661 ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc ttctcggatc aatggggact
     6721 gcataaccag ctggcggtgt ttaacccagc accggtgacc tgatttcttt tggaacgtcc
     6781 ctcgcaaaac atggccttag tgccatgttt ttattgttta aagcccccac gtccattaat
     6841 aatgcatttg cattacctta gttcaagctt ataatttgag cagaaaacag gctgtaaaag
     6901 gacagtgaat catgcccgct aatgctcgct ctcacgctgt actgaccact gaatcaaagg
     6961 tcacgatacg cggacaaaca actatccccg cgccagtgcg tgaggcctta aaactgaagc
     7021 caggccagga cagcattcat tacgaaattc tgcctggtgg gcaagtattt atgtgccgac
     7081 tgggagatga gcaggaggat catactatga atgcattttt gcgttttctg gatacagata
     7141 tccagaacaa cccgcaaaaa actcgtccat tcaacattca acaaggaaag aaacttgtcg
     7201 ctggcatgga cgtcaacatt gatgatgaga ttggcgacga cgaataatgg attttccaca
     7261 aagggttaat ggttgggcgc tatatgctca tccctgtttt caggaaacct acgacgcttt
     7321 agttgccgaa gtcgagacat taaagggaaa agatcctgaa aattatcaga gaaaagctgc
     7381 cacaaagtta ttggcggtgg tccataaagt gattgaggag catatcacgg tcaatccatc
     7441 atcaccggca ttccgtcatg gcaagtcgtt aggctctggg aaaaataaag actggtcacg
     7501 ggtaaaattt ggtgctggtc gctatcgtct cttctttcgt tacagtgaaa aagagaaagt
     7561 catcattctg ggatggatga acgatgaaaa cacgctgcgc acctacggta aaaaaacaga
     7621 tgcctatacc gtattcagca aaatgttaaa aagaggacat cctcctgccg actgggaaac
     7681 cctcacccga gagacagaag aaacccattg atggtgttca caatgcccgc cgaacgcatt
     7741 cgtgttgacc agcgggcacc ctttctcact ccccgaccag aatcacttca accccagcct
     7801 ttcgcagccc ttccaggcta tccgcaggaa tgccttcatc aacaatgatc atgtcgatac
     7861 gttgagtatc aatgatctta tgtaaactgg aacgattgaa tttactggaa tcggtgacca
     7921 cgatgatccg ttccgcaact tcgcacatcc ggcggtttaa acgggcttca tcttcattat
     7981 gcgtgctgac gccgcgctcc agatcgatcg catctacacc aagaaacagc atatcgaagt
     8041 ggtaattttg cagcgattgc tcagcctgat cgccgtaaaa agattgcgac tgacggcgca
     8101 aatgcccgcc ggtcatcagc agctcaacgc cttccgcttc cagcaacgca ttagccacgt
     8161 tcataccgtt ggtcatcgca attacgtcag tgtgcttgcg catcagacga gcaatctcaa
     8221 aagtggtggt cccggaatcg aggatcaccc gatgacctgg ctgaatcaac tcaacggcag
     8281 ctttcgcaac gctgcgtttc attgcggtgt tcagcgcgct tttatcttcc actgatggct
     8341 cgactgacgg cgttgtgcta tcgcagatca acgcgccacc ataggcacgc acagcgatcc
     8401 cctgcttttc cagaaacgcc agatcgttgc ggatcgtcac ggtagatacg ccatacaatg
     8461 ccgacagatc gttaacctgc acactccctt gctgtcgcag acgctgaatg atctgttctc
     8521 gtcgctcgct ggtgcctgtc actcgcttct cacctgaagc gtcggtatta ctcatagtaa
     8581 gtcctttcgt aaaactttcg tttcatttcg ttttgcctat taacgccttt ctattaagca
     8641 aatgcaagcc caccttgccc attgacgcaa gctactctcg tttcagtgac tttcattatg
     8701 tttcttttgt gaatcagatc agaaaactat tatctttcgt tttattttta tctcaccatg
     8761 acgcagtatc aactgaaaca aaacgaaaga ttaatatcgc agtaatctga actggagagg
     8821 aaagtgaaac atctgacaga aatggtgaga cagcacaaag cgggcaaaac aaatggaatt
     8881 tatgccgttt gttccgcaca tccgctggtg ctggaggctg caatccgcta cgccagtgca
     8941 aaccaaacgc cgttactgat tgaagcaacc tccaatcagg tagaccagtt cggcggttat
     9001 accggaatga cgcccgccga ttttcgcggc tttgtttgtc agctcgccga ctcgttgaat
     9061 ttcccgcagg atgcgttgat tctgggtggt gaccatctgg ggccaaaccg ctggcaaaac
     9121 ctgccggccg ctcaggcaat ggccaatgcc gatgatttga ttaaaagcta cgttgcggca
     9181 ggattcaaaa aaatccacct tgattgcagc atgtcctgtc aggacgatcc gattccctta
     9241 actgatgaca tcgtggctga acgcgccgcc cgtctggcga aagtggcgga agaaacctgt
     9301 cttgaacact ttggcgaagc cgatctggag tatgtcattg gtaccgaagt gccggtacct
     9361 ggcggcgcgc atgaaacctt aagcgagctg gcggtcacca cgccggatgc cgcccgcgcc
     9421 acgctggaag cccatcgtca cgcctttgaa aagcaaggtt tgaatgccat ctggccacgc
     9481 atcattgccc tggtggttca acccggcgtc gaattcgatc acaccaacgt tattgattat
     9541 cagcccgcca aagcgaccgc cttaagccag atggtcgaaa gctacgaaac gctgattttc
     9601 gaagcgcact ctaccgatta tcaaacgccg caatcgctgc gccagctggt gattgaccac
     9661 tttgccattc tgaaagttgg cccggcgctg accttcgccc tgcgtgaagc tctgttctct
     9721 ctggcagcga ttgaagaaga actggtgcca gcaaaagcct gttctggtct gcgtcaggtg
     9781 ctggaaaacg tgatgctcaa ccgcccggaa tactggcaaa gccactacca cggtgacggc
     9841 aacgcgcgtc gtctggcgcg tggttatagc tactcggatc gcgtgcgcta ttactggccg
     9901 gacagccaga ttgatgacgc tttcgctcat ctggtacgta atctggcgga ttcaccaatt
     9961 ccgctgccgc tgatcagcca gtatctgccg ctgcagtacg tgaaagttcg ctccggcgag
    10021 ctgcagccaa cgccacggga actcattatc aaccatattc aggacatcct ggcgcagtac
    10081 cacacagcct gtgaaggcca ataagcaaaa caaagaggaa cacgctatgc caaatattgt
    10141 tttaagccgg attgatgaac gcttgattca cggtcaggtc ggcgttcagt gggtcggatt
    10201 tgcgggggca aatctggtgc tggtcgccaa cgatgaggtt gccgaagatc cggtacaaca
    10261 aaacctgatg gaaatggtac tggcagaagg gatcgccgta cgtttctgga cgctgcaaaa
    10321 agttatcgac aacattcatc gcgccgccga tcgacagaaa atcctgctgg tttgtaaaac
    10381 acccgccgat ttcctgacgc tggtgaaagg tggcgttccg gtgaatcgca ttaacgttgg
    10441 caatatgcac tacgccaatg gcaagcaaca aatcgccaaa acggtttctg tggatgcggg
    10501 cgatatcgca gcatttaacg acctgaaagc cgctggggtg gaatgcttcg ttcagggcat
    10561 cccgacagag cctgctgtgg acctctttaa attactttga gggattcatc atggaaatca
    10621 gtctgttgca ggcattcgcg ttgggcatta tcgcctttat cgctggcctg gatatgttta
    10681 acggcttaac ccatatgcac cgcccagtgg tcctcggccc gttagtcggg ctggtacttg
    10741 gcgatctgca taccggtatt ttaaccggcg gtacgctgga actggtgtgg atggggctgg
    10801 ccccgctggc gggcgcacag ccgcctaatg tgattatcgg tactatcgtc ggcacggcgt
    10861 ttgccattac tactggcgtg aaacccgatg tcgcagtcgg tgtcgccgta cctttcgctg
    10921 tcgcagtaca gatggggatt accttcctgt tctcggtgat gtctggcgtg atgtctcgct
    10981 gcgaccggat ggcggagaac gccgatactc gcggcattga acgcgtaaac tatctggcac
    11041 tgctggcact cggcactttc tattttctct gcgctttcct gcctatctac ttcggcgcgg
    11101 aacatgcaaa aaccatcatt gatgtcctgc cgcaacgatt aatcgacggc ctcggcgtcg
    11161 cgggcggcat catgccagca atcggttttg ccgtgctgct gaaaatcatg atgaaaaacg
    11221 tctacatccc ctacttcatc ctgggctttg ttgccgcagc ctggctcaag ttaccggtgc
    11281 tggctattgc tgccgccgcg ctggcgatgg cgctgatcga tctgctgcgt aaatctcctg
    11341 aaccgactca acctgcggca cagaaagagg aatttgaaga tggcatctaa tcaaaccacc
    11401 ctgccgaacg tcagtgaaaa cgaagaaaca ttgctgactg gcgtcaatga aaacgtgtat
    11461 gaagatcaga gcattggcgc ggagctgacg aaaaaagata tcaatcgcgt cgcctggcgt
    11521 tccatgctgt tacaagcgtc ttttaactac gaacgtatgc aggcttccgg ctggttgtac
    11581 ggtctgctgc ctgctctgaa aaagatccac actaataaac gcgacctggc gcgcgccatg
    11641 aaggggcata tgggtttctt caatacccat ccgtttctgg tgacatttgt tatcggcatt
    11701 atccttgcga tggagcgttc taagcaggac gttaacagta ttcagagcac caaaattgcc
    11761 gtcggtgcgc cgctcggcgg gattggcgat gcaatgttct ggctaacgct actgccgatt
    11821 tgtggcggga ttggagccag tctggcattg caaggctcca ttcttggcgc agtcgtcttt
    11881 attgtgctgt tcaacgtggt ccacctgggg ctgcgttttg gtctggcgca ttatgcttac
    11941 cgcatgggcg tggcggcgat tccactcatt aaagctaata ccaaaaaagt cggccatgct
    12001 gcgtctatcg ttgggatgac ggtaatcggt gcgctggtgg caacctatgt tcgtttaagc
    12061 accacgctgg aaatcacagc gggcgacgca gtggttaagt tacaggctga tgttatcgac
    12121 aaactgatgc ctgccttctt accgttggtc tacaccctga ccatgttttg gttggtacgc
    12181 cgcggctgga gtccgctgcg tctgattgca gttaccgtgg ttctcggcat cgttggtaaa
    12241 ttctgccatt tcctttaaat acaatgaggt attagatgtt aagtattatt ttgacagggc
    12301 atggcgggtt tgccagcggc atggaaaaag caatgaagca aattctcggt gagcaatcgc
    12361 agtttatcgc catcgatttt ccggaaacct caagcaccgc gctgcttaca tcgcagcttg
    12421 aagaggccat cgcccaactg gattgtgaag acggcattgt ttttctgacg gatttgctgg
    12481 gcggcacgcc gtttcgcgta gcttcaacgc tggcgatgca aaaaccgggc tgtgaagtga
    12541 tcaccggcac caatttgcaa ctgctactgg agatggtgct ggagcgcgaa gggttaagcg
    12601 gtgaagaatt ccgtgtacag gcgctggagt gtggacatcg tgggctgacc agtctggtgg
    12661 acgagttagg ccgctgccat gaagaatgtc cggtcgagga agggatatga cacacgttct
    12721 gcgcgccaga aggctgctga ctgaagaggg atggctcgat gaccatcagt tgcgtattgc
    12781 tgacggtgtc atcgcagcaa tcgaaccgat tccagtgagc gtgactgaac gcgatgcgga
    12841 actgctctgc cccgcttaca tcgacaccca tgtacacggt ggtgcgggcg ttgatgtaat
    12901 ggatgacgcg ccggatgtac tcgacaagct ggcaatgcac aaggcacgcg aaggtgtcgg
    12961 cagttggttg ccgactaccg taaccgcgcc gcttagtacc attcatgcgg cgctgaaacg
    13021 tattgctcaa cgttgccaac gcggcggacc tggtgcgcaa gtgctgggga gttatctcga
    13081 aggaccgtac ttcacgccgc agaataaagg cgcgcatccg ccggagttgt ttcgcgagct
    13141 tgaaattgcc gagctggatc aattgattgc cgtttctcag cacaccttac gcgtggtagc
    13201 gctggcaccg gaaaaagagg gggcattgca ggccatccgc catcttaaac agcaaaatgt
    13261 acgagtgatg ctggggcata gcgcggcgac ctggcaacaa actcgcgccg cgtttgatgc
    13321 tggtgccgac ggcctggtgc attgctataa cgggatgaca ggtttacatc accgcgaacc
    13381 gggaatggtt ggcgcgggat taacggacaa gcgcgcctgg ctggaactga tagccgatgg
    13441 tcatcatgtg catccggcgg caatgtcgct gtgttgttgc tgtgcgaaag agagaatcgt
    13501 actgatcacc gacgcgatgc aggcagccgg gatgccggat ggtcgctata cgttatgtgg
    13561 cgaagaagtg cagatgcacg gtggcgttgt ccgtaccgcg tccggtgggc tggcgggcag
    13621 tacgctgtct gttgatgcgg cagtgcgcaa catggtcgag ttgacgggcg taacgcctgc
    13681 ggaagccatt catatggcgt cgctgcatcc ggcgcgaatg ctgggtgttg atggtgttct
    13741 gggatcgctt aaaccgggca aacgcgccag cgtcgttgcg ctggatagcg ggctgcatgt
    13801 gcaacaaatc tggattcagg gtcaattagc ttcgttttga tggtttgctc cattattggg
    13861 ccttcacttc ccccgtaagg cctttctttt tctttcgttt tgatctgtgc agcggtgtcg
    13921 gatgcgacgc taacgcgtct tatccgacct acagttggtg accgcaaggc cggataaagc
    13981 gtttgcgccg catccagcaa tcccttttgc ttcctttatc ttttctttca acgatcacgg
    14041 atttcgtttt atttcctttt tctccattga actttcggtt tcttttctat agattttaat
    14101 caacgaaaga catcaccaag tgaaatgaaa cgaaaggcaa gtgaaagcga caacgcccga
    14161 cgtcaagttc atcagactaa ggattgagtt atgccagaaa attacacccc tgctgccgcc
    14221 gcaaccggta catggactga agaagagatc cgccatcagc ctcgcgcatg gatccgttcg
    14281 ctcaccaaca tcgacgcgct acattccgcg cttaataact tccttgaacc gttactgcgc
    14341 aaagagaatc tgcggatcat cctgaccgga gccggaacct cggcatttat cggtgacatc
    14401 atcgcgccgt ggctcgccag ccataccggt aaaaacttca gcgccgtacc gaccaccgat
    14461 ctggtcacta atccgatgga ctacctgaac ccagcccatc cgctgctgtt gatctccttc
    14521 ggtcgatccg gcaacagccc ggaaagcgtc gcagccgtgg aactggcaaa tcaatttgta
    14581 ccggaatgct atcacctgcc gatcacctgc aacgaagcgg gcgctcttta ccaaaacgcg
    14641 atcaacagcg ataacgcgtt tgccgtgctg atgcccgcag aaacgcacga tcgcggcttt
    14701 gcgatgacca gcagcattac caccatgatg gccagctgcc tcgcggtttt cgcacctgag
    14761 acgatcaaca gccaaacctt ccgcgacgtg gcggatcgtt gccaggcgat cctgacctca
    14821 ctgggcgatt tcagcgaagg tgtgtttggt tacgcaccgt ggaaacggat cgtttatctc
    14881 ggcagcggtg gcttacaggg cgcagcacgc gagtcggcgc tgaaagtgct ggaactgacg
    14941 gcgggtaaac tggcggcctt ttatgattct ccaaccggat tccgtcatgg accaaaatcg
    15001 ctggtcgata acgaaacact ggtggtggta tttgtctcca gccaccctta cacccgtcag
    15061 tatgatcttg atctgctggc tgaacttcac cgtgacaacc aggcaatgcg tgtaatcgcc
    15121 atcgccgcgg aaagcagcga catcgtcgct gccggtccac atatcatcct gccaccgtca
    15181 cgtcacttta tcgacgttga gcaggcattt tgcttcctga tgtacgccca gacgtttgca
    15241 ctgatgcagt cgctgcacat gggcaatacg ccggataccc catcagccag tggcaccgtt
    15301 aaccgcgtgg tgcaaggcgt aatcattcat ccgtggcagg cataagagga tcgcattatg
    15361 agcattatct ccaccaaata tctgttacag gacgcccagg ccaatggcta cgcggtgcct
    15421 gcttttaaca ttcataacgc cgagacgatc caggcgatcc tcgaagtgtg cagtgaaatg
    15481 cgatcgccgg tgatcctcgc cggaacgccg gggaccttta aacatatcgc gctggaagag
    15541 atctacgccc tgtgcagcgc ctattccaca acctacaaca tgccactggc gctgcatctc
    15601 gaccaccacg aatcgctgga tgatattcgc cgtaaagtcc acgcaggtgt gcgcagtgcg
    15661 atgatcgacg gcagccactt cccgtttgcc gagaacgtaa agctggtgaa atcggttgtc
    15721 gacttctgcc actcccagga ttgcagcgtg gaagcagaac tgggccgcct gggcggtgtt
    15781 gaagatgaca tgagcgttga cgccgaaagt gcattcctga ccgatccaca agaagctaaa
    15841 cgctttgtcg aactgactgg cgtcgacagc ttggcggtag cgattggtac ggcgcacggc
    15901 ttatacagca aaacgccgaa gattgatttc cagcggctgg cggaaattcg tgaagtggtg
    15961 gatgttcctc tggtgctaca tggtgccagc gatgttccgg atgaatttgt ccgtcgcact
    16021 attgaacttg gcgtcacaaa agtgaacgtt gccacagaat taaaaatagc cttcgccggc
    16081 gcggttaaag cctggtttgc ggaaaatccg cagggtaatg atcctcgtta ttatatgcgc
    16141 gtcggaatgg atgcgatgaa agaagttgtc agaaataaaa ttaatgtctg tggttcagcg
    16201 aatcgaattt cagcataatc attagttttt tatgatttat cccaatgtac ttcccgatta
    16261 atatcgggga gtgccttaat ggaaaaggag atgactaaac cttaataaat agatcagtac
    16321 aatatcgcaa caataatata tttaaaaaaa ttatattatt caactttatg gtgaggatta
    16381 cacaatgagc agtccaaata ttctcttaac ccgtattgat aaccgtctgg ttcacggtca
    16441 ggttggcgtg acctggacat ccaccatcgg tgcgaatctg ctggtagtcg tggatgatgt
    16501 tgtcgctaac gatgatattc aacagaaatt aatgggtatt accgcagaaa cctacggctt
    16561 tggcattcgt ttctttacta tcgaaaaaac cattaacgtc atcggcaaag ctgcaccgca
    16621 tcagaagatc ttcctgattt gccgtacgcc acaaacggta cgtaaattgg tagaaggtgg
    16681 cattgacctg aaagatgtca acgtcggcaa tatgcatttc tcggaaggga aaaagcaaat
    16741 cagcagtaaa gtttatgtcg atgaccagga tctcacggac ttacgtttta ttaaacaacg
    16801 tggcgtgaat gttttcattc aggacgtccc tggcgatcaa aaagaacaaa tccctgacta
    16861 aatctaaaat cgccttaata ttggtttgag gtaataaaaa tgcatgaaat aaccctactt
    16921 tagggattat ccctggcggc gttagttttc gttctgggga ttgatttttg gctggaagcc
    16981 ttatttttat tccgcccgat aatcgtttgt accctaactg gcgctattct cggtgatatt
    17041 cagactggct taattaccgg tggtctgaca gagttggctt tcgccggatt aacccctgca
    17101 ggtggtgttc agccgcccaa cccgattatg gcgggtctga tgaccaccgt cattgcatgg
    17161 tctacgggcg ttgatgccaa aacggcaatt ggtcttggcc tgccgtttag tttgttaatg
    17221 cagtacgtca ttctgttctt ctattctgct ttctcattat ttatgaccaa agccgataaa
    17281 tgcgcgaaag aggcggatac ggcagcgttt tcccgactta actggacaac gatgctcatc
    17341 gtcgcttcag cgtatgcggt gattgctttc ctctgtactt acctggcaca aggggcgatg
    17401 caggcgctgg tgaaagcgat gcccgcttgg ctgacccacg gctttgaagt ggctggcggt
    17461 attctgcctg ccgttggttt tggcttgctg ctgcgcgtga tgttcaaagc acaatatatc
    17521 ccttacctga tcgccggctt cctgtttgtt tgctacatcc aggtcagcaa cctgttgccg
    17581 gttgccgtac tgggcgcagg ctttgcggtg tatgagtttt tcaatgcgaa atcccggcag
    17641 caagcgcaac cgcagcccgt tgccagtaaa aatgaagaag aggactacag caatgggatc
    17701 tgaaatcagt aaaaaagata tcacccgtct gggctttcgt tcgtcgctgc tgcaagcgag
    17761 ctttaactac gaaaggatgc aagcgggcgg ttttacctgg gcgatgttgc cgatcctgaa
    17821 aaagatttat aaggatgaca aaccgggcct aagcgcagcg atgaaagata acctcgaatt
    17881 tattaatacc cacccgaatc tggtcggatt cctgatgggg ttattaattt cgatggaaga
    17941 aaaaggagaa aaccgcgaca ccattaaagg cctcaaagtg gcactgtttg gcccaatcgc
    18001 cgggattggc gatgcgattt tctggtttac tttgttgccg attatggcgg gaatttgctc
    18061 atcatttgcc agccagggaa acctgctggg gccgattcta tttttcgccg tttacctgct
    18121 tatctttttc ctgcgcgtcg gctggaccca cgtcggttat tcagtcggcg tgaaggcgat
    18181 cgataaagtg cgagagaact cgcagatgat tgctcgctcg gcaaccatcc tcgggatcac
    18241 ggtaatcggc gggctgatcg cttcgtatgt gcatattaac gtggtgacat cgtttgccat
    18301 cgacagtacc cacagcgtcg cgctgcaaca ggatttcttc gataaagtct tcccgaacat
    18361 tttaccgatg gcctacaccc tgctgatgta ttacttcctg cgggtgaaaa aagcgcatcc
    18421 ggtgctgtta atcggcgtga cttttgtgct ctctattgtt tgttccgcat tcggcatttt
    18481 gtaaatggaa cgaggcactg cgtctggcgg tgcctcttta cttaaggaat ttcatcctgt
    18541 gcaaaccctt cagcaagttg aaaactatac ggcgttaagt gaacgtgcca gcgaatattt
    18601 attggccgtg atccgtagca aaccggatgc cgtgatttgc ctggcgaccg gagccacgcc
    18661 attactgacg tatcattatc tggtagaaaa aatccactag cagcaggttg atgtcagcca
    18721 gctcaccttc gtgaagctcg acgaatgggt ggatctgcca ttaacgatgc ctggtacctg
    18781 cgaaactttc ctgcaacagc atatcgtgca gccgctgggg ctacgtgaag accagctcat
    18841 cagctttcgc tccgaagaga taaatgagac agagtgcgaa cgggtaacga acctgattgc
    18901 gcgcaaaggc ggtctggatt tatgcgttct cggattgggg aaaaacggtc atcttgggct
    18961 gaacgaaccg ggagaaaacc tgcaaccggc ctgccatatc agtcaacttg atgccagaac
    19021 acagcaacat gagatgttaa aaaccgcggg tcgccccgtg actcgtggga tcaccttagg
    19081 cctgaaggat attctcaatg cccgcgaggt tttgttactg gtgactggcg aaggaaagca
    19141 ggatgcgaca gaacgttttc tcacggctaa agtctctacc tctatcccgg cttcgttttt
    19201 gtggctgcac agtaacttta tttgtttaat taatacctaa ccagatgaaa aatttgctca
    19261 aaatgtttaa attaacttat gcaacagtca cgcattatat taaataacat ttgactgggt
    19321 tgaacataac gccaatagca aaaggagtta tgttcaacaa tttaatttta gatatacata
    19381 taacttgttc tattaataaa ccatctcgta ttctgactcg ccccctggcg cagcaaagtc
    19441 atgcacttat actc
//
LOCUS       DAZFDC010000090        19079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFDC010000090 DAZFDC010000000
VERSION     DAZFDC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.090"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..258)
                     /locus_tag="ACHJ48_003272"
     CDS             complement(<1..258)
                     /locus_tag="ACHJ48_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708763.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLPEENYRRALYIL
                     SIIYPIKLNVGDDEFKFIFYIMSQKKFLRQQTISDFVRSINV"
     gene            complement(404..4306)
                     /gene="hrpA"
                     /locus_tag="ACHJ48_003273"
     CDS             complement(404..4306)
                     /gene="hrpA"
                     /locus_tag="ACHJ48_003273"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO4708764.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIGHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWFNKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4507..5112
                     /gene="azoR"
                     /locus_tag="ACHJ48_003274"
     CDS             4507..5112
                     /gene="azoR"
                     /locus_tag="ACHJ48_003274"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO4708765.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTTRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5163..6455)
                     /gene="ynbD"
                     /locus_tag="ACHJ48_003275"
     CDS             complement(5163..6455)
                     /gene="ynbD"
                     /locus_tag="ACHJ48_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO4708766.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDFNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIVLCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISMLTTWQHHFIDVITGLAVGMLIDWMIPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6469..8226)
                     /gene="ynbC"
                     /locus_tag="ACHJ48_003276"
     CDS             complement(6469..8226)
                     /gene="ynbC"
                     /locus_tag="ACHJ48_003276"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO4708767.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYHRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPGNEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8242..9138)
                     /gene="ynbB"
                     /locus_tag="ACHJ48_003277"
     CDS             complement(8242..9138)
                     /gene="ynbB"
                     /locus_tag="ACHJ48_003277"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO4708768.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFSC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLTT
                     TNTQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(9138..9743)
                     /gene="ynbA"
                     /locus_tag="ACHJ48_003278"
     CDS             complement(9138..9743)
                     /gene="ynbA"
                     /locus_tag="ACHJ48_003278"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO4708769.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYVGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9914..12220)
                     /gene="ydbD"
                     /locus_tag="ACHJ48_003279"
     CDS             complement(9914..12220)
                     /gene="ydbD"
                     /locus_tag="ACHJ48_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO4708770.1"
                     /translation="MFKPRLCSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRLKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALKLVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12284..13144)
                     /gene="pdxI"
                     /locus_tag="ACHJ48_003280"
     CDS             complement(12284..13144)
                     /gene="pdxI"
                     /locus_tag="ACHJ48_003280"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO4708771.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEKV
                     LSTLDGISRE"
     gene            complement(13364..15823)
                     /locus_tag="ACHJ48_003281"
     CDS             complement(13364..15823)
                     /locus_tag="ACHJ48_003281"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO4708772.1"
                     /translation="MNEGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVIN
                     NTGSLSTLQLHDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGT
                     WKMGYQDENNNAGTLDIDDKSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEMTLD
                     AALGAGAIHYDDGASEFINKGVVDAKVTVAVSTAGATESDAFLWNQDGGVINFDKDNA
                     SAVKFTHNNYVALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLTGMQ
                     LDANATADAVIENNGTINIFANDSFAFSVLGTEGHIVNNGTVVIADGVTGSGLIKQGD
                     SVNVEGVNGNSGNNTEVHYTDYTLPDMPNTYTTSPFSETTDSGSSDGSSNNLNGYIVG
                     TNVDGSAGKLKVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVV
                     WTAKGSTDASGNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAE
                     LNSALKQVSGSQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNN
                     TEYDMLALRKTVDLSESQSMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSF
                     DNGMRWNNALRYDVHNLDSSRSVAYGDVSKTADTDVKQQYLELRSEGAKTFEPREGLK
                     ITPYAGVKLRHSLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKGGWSANAT
                     LEGGPNLSYSKSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDA
                     YHWKEDGISDKGVMLNFKKTF"
     gene            complement(16043..19078)
                     /locus_tag="ACHJ48_003282"
     CDS             complement(16043..19078)
                     /locus_tag="ACHJ48_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HFO4708773.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVP
                     PDDGGDTPVKHNPVVYKNGVTWDQDAKTVKIRETTFTYSQNADGSYTLTAPEGKETIV
                     KSWTVHDDSNTVVFDGVNTSGGITWSYDDDGLIHITKEAGVVVDGTTGNAIEFGNAII
                     TDQGGNTALNGGTVMTVDGDNISLNNDGKTTAIGEGSVVGILTGDNITINNNGETEVD
                     GGTAVIINGDNTKLNTAGDSTITNGGTGSLINGDNARVDNQGTMSVDGENSTGSKIVG
                     DGATIKQEGDLYVSGGAHGIDVDGNDTFVSNKGNITVIEDNSIGMLLDGDGVSVINMG
                     DLNVGQAAAGENAIGIQIDGDNATFVNVGDISATNAGTGVSVAGDKANISLAGGLDVG
                     DFSTGLDVSGNNNNMTLATYELNVTGQKATGVNVSGDGNTIEIAGSILVDKDQKADNA
                     QPYFFNPSTGVNISGDNNDVTLDGQLTVVADSKTTSRSYASYDGAQEHIAGIVIAGDD
                     NTFTLNGGVHFVGEKNVMDDGSKPSASRRGIGDTPLINVDGHSPVYLNGESTISGEFP
                     LGFENLIQLSHGAELEIGADATFDMSDVDSFTYYYRVALSTISIDSGAKATNNGEVEL
                     KNIGFAAAWNKDSTVINNGSIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTMTTKMMNQ
                     HSVLNYPAEWNLSDGTSFNNKALGLTGMLASYSSSILNGETGIIDMYGRGSVGMLAID
                     KSTADNEGQITLDTLWVDENDETSLRNNVANSTAKDFGVGMASGTDAYNGALTKATAT
                     NHENGVITVYNAGAGMAAYGNTNTVINQGTINLEKNENYNDSLGVNKLVGMAVYHEGT
                     AINDQTGVININAENGQAFYNDGTGLIINYGTICTFGVC"
BASE COUNT         4806 a         5123 c         4312 g         4838 t
ORIGIN      
        1 aacatttatt gagcgtacaa aatcgctaat ggtctgttgg cgtagaaatt ttttctgtga
       61 catgatgtaa aaaataaact taaattcgtc gtcaccgacg ttaagtttaa tcggataaat
      121 tattgataaa atatacagcg cgcgtcggta attttcctct ggaagttttt catagaaaga
      181 tattaaagcc tgaccaacgc gatcaccctc tattatccgt tcaaattgcg acctcagtaa
      241 aatatcatgt ctactcattg caatacgtcc tgttcgccca aggcgaagta tgaatgaaac
      301 ttatgtaaaa tagtaccgcc cttgctatat ttcagaattc ataaagaaaa aacccggcgc
      361 taaatgctta ccgggtttta gatttatcag gcaaatagca gggttaaccg ctaatctgct
      421 ccatcgcctg caaaatacgc ttatctgaaa tcggataagg cgtaccaagt tgttgagcga
      481 agtaactaac gcgcagctct tctatcatcc aacggatctc tttcacgtct tcatcctcac
      541 gacgtgcagg cgggagtttg ttgaaccatt gctgccacgc ctgctggacg ttttcgactt
      601 tcagcatctg tgcacgatcg cgatgtggat caaccgccag tttttccagc cgtttttcaa
      661 tcgcctgcaa ataacgcagc gtgtcgccca gccgtttgaa gccgttacca gtgacaaaac
      721 cgcgatatac caacccgccc atctgcgctt taatgtcaga aagccccagc gccatggtca
      781 tatccacccg ccctttcaga cgtttgttga tattgaacac tgccgtaagg atttgctcga
      841 cctgcttcgc aatatccacc accgtgtcgt tcagttcggc acgcactttt tcatgcagcg
      901 cagcaaagcc ttcttccgtc cagaccgggc ctccattggc gtcgatcagt tgatccacac
      961 cgcatgagat acagtcgtcg atcagctcca gcactttgcc atacgggtta aagtacagcc
     1021 ccagcttggc tttgtttggt aacttctcat gtaaatactt gattggtgag ggaatattca
     1081 gcagcagtag acggcgaaga ccgttccaca ttgcctgttt ttgctccagc gggttatcaa
     1141 acagtttgat cgccacactg tcgcgctcat ccaccagcgc cggccacgcc ttcactttgt
     1201 agttaccacg cttctgttcg tagctttccg gcaactgacc aaaactccag atatgtaagc
     1261 cgctctgctc gataccgtca tccgccaccg cagatagcgt ttcctgcact ttgcctttca
     1321 gcgcatcttt cagatcttgt agcgagcgcc cttcttttag cttcttgttt ttgtcatcca
     1381 ccacgcggaa ggtgattttc aggtgatcgg gcacctgatc ccagtgccag tcttcgcggt
     1441 caacggtaac gccggtcatc cgccgtaact cgcgctcaag gctgtcgagc aacggtaact
     1501 ccagcggtgt gacgcggcct aaaaacgctt cggcatagtt tggcgcaggt acaaaattac
     1561 ggcgtaccgg tttcggcaac gatttaatca gagcaatcac cagttcgcgg cgcagaccag
     1621 ggatctgcca ttcaaacccg ctttcctcaa cctggttaag taacggcagc ggaatatgta
     1681 cggtcacacc gtcagcatcc gcgccgggct caaactgata gctcaaacgc agcttgagat
     1741 tgccctgatg ccagaagttc gggtaatcca gcttgctgat cttttccgcc ccttctttga
     1801 tcaacatgct cttttcaaag ttgagcaaat caggcgtttc gcggctgact tttttccacc
     1861 agctgtcgaa gtgacgagcg gagattacat cgtggccgat gcgctggtcg tagaactcaa
     1921 acagcgtttc gtcatcaacc agaatatcgc ggcgacgtga tttgtgttca agctcttcca
     1981 cttcggcccg cagtttcagg ttttcacgga agaatgcgtg acgcgtctgc cagtcacctt
     2041 ccaccagcgc gtggcgaata aagagttcac gacataacgc cggatcgatc tggctgtagt
     2101 tgaccttgcg cgcggcaaca atcggcaaac cataaacagt gactttttcc gttgccatca
     2161 ccgcgccctg cgcccgttcc cagtgcggtt cgctgtaggt acgtttaatc aaatgctgag
     2221 cgacaggctc cacccattcc gggtcgatac gcgcagcaat gcgcccccac aggcggctgg
     2281 tttctaccag ttccgccacc attacccatt tcggcggttt tttgaataaa ccagaaccgg
     2341 ggaagatgga gaaacgcgcg ttacgtgcgc cggtatattc ttgtttatcg gcatctttca
     2401 tgccgatatg ggaaagcaac cccgtcagca acgcaatgtg aatttcacga tactccgccg
     2461 gttcgctgtt caccggaatg ccgagttctt tcaccacctg gcgcaactgg gtgtagatat
     2521 cctgccattc gcgcacacgc agatagttga gataatcggt acgacacagg cgacggaagg
     2581 cgttggaaga aagcgccttt tgctgctcgc caagataatt ccacagattc acaaacgcga
     2641 ggaagtcaga ctctttgtcg tggaagcgac gatgtttttc gtccgatgcc tgctgtttgt
     2701 ccatcggacg ttcgcgcgga tcctgaatgg agagcgcaga cgtgataatc atcgcctcac
     2761 gcacgcagcc atgtttttgc gcttccagca ccatacgcgc cagacgtggg tcgacaggca
     2821 actgcgagag ctggcgaccg agcggcgtca gtttataggc gctggcctgt tcatccgtgg
     2881 tgatcgcgcc cagttcttcg agcagacgca cgccatcctg gatattgcgt ttatccggtg
     2941 cttcgacaaa cgggaacgca gcgatatcgc ctagccccag cgcggtcatc tgcaaaataa
     3001 ccgaggccag gttggtacgc agaatctccg gatcggtaaa ctccgggcgc gagaggaaat
     3061 cgtcttcgga atagagacga atacagatcc cttcggacac acgaccacag cggcctttac
     3121 gctgattggc agacgcctgc gaaatgggct caatcggcaa acgctgcact ttggtgcgat
     3181 agctgtagcg gctgatacgc gccgtaccgg ggtcgataac gtatttaatc cccggtacag
     3241 tcagcgaggt ttccgcgacg ttggtcgcca gcacaatgcg tcgcccgctg tgcgactgga
     3301 acacgcggtt ctgttcgcta ttcgaaagcc gcgcataaag cggcaagatt tcggtatggc
     3361 gtaagttcag cttgttcagc gcatcggcgg tatcgcgaat ttcccgctcg ccgctcataa
     3421 agatcagaat gtcgccgggg ctttcctggc ttagttcgtc cacggcatcg aaaattgcct
     3481 gcaactggtc gcgctcagtg tcgtcggctt cttcaacaat cgggcgatag cgcacttcca
     3541 ccggataggt ccgaccggag acttcaataa tcggcgcatt attaaagtgg cgcgaaaagc
     3601 gttccgggtc gatagtcgcg gaagtgataa tgatttttag gtcaggacgc cgcggcagca
     3661 actctttcaa ataaccgagc agaaaatcga tattcaggct acgttcgtgc gcttcgtcaa
     3721 taatgatagt gtcgtactgc atcagcaggc ggtcttgctg gatctccgcc agcaggatac
     3781 cgtcggtcat tagcttgacc atcgtgttat cacttacgtg atcgctgaaa cgcactttgt
     3841 aaccgatgca accgcccggc tccgttttca gctcttccgc aatacggttc gctaccgttc
     3901 ttgccgccag gcgacgcggc tgggtatggc cgatcagccc tttaatcccg cgccccagct
     3961 ccatacagat tttcggtaac tgagtcgttt taccagaacc cgtttccccg gcgactatca
     4021 ccacctggtg atcacgaatc gcttcgagaa tgtcctgttt tttctgacta accggcaaat
     4081 tgtcaggata agtaatttcc ggtcgtgccg cttcacgcag caggactttc cctgccgcct
     4141 ggtcaatctc tttcgccatc tcctggaaaa tggcctgttg tgcatcagga tttttaacct
     4201 tcttcacgcc gtgcagacgg cgagaaaaac gcagtctgtc acgcagcatc agcgaatcca
     4261 gccgctgctg caaggccgta aaagtcaatt tttgttgttc tgtcataacg ttagtgggca
     4321 gtagtctgcc tgattaattt tctttgaaat gatagatata gagtaccaca tcgcggcttt
     4381 ctatgcgttg ttcaataaat tcgaacatag gcttcgattt attgcgctat ctctgtggca
     4441 ggattgtgaa taaagtgtca acaagcaacg gggcatcgcc cattcaaata tctataagga
     4501 aacaccatga gcaaggtatt agttcttaaa tccagcatcc tggcagggta ctctcagtct
     4561 aatcagttgt ccgattattt tgttgaacaa tggcgcgaaa agcactccgc tgatgaaatc
     4621 accgttcgcg acctggctgc aaatccaatt ccggtactgg atggcgaact ggttggcgct
     4681 ctgcgtccga gcgatgcgcc gctgactacg cgtcagcagg aagctctggc actttccgat
     4741 gagctgattg ccgagctgaa agcccatgac gttatcgtta ttgcggcacc gatgtataac
     4801 ttcaacatct cgactcagct gaaaaactat tttgacctgg ttgcacgtgc aggcgttact
     4861 ttccgctata ccgagaatgg tccggaaggt ctggtaacgg gtaaaaaagc catcgttatt
     4921 accagccgcg gtgggatcca caaagatgga ccaacggacc tggtgacgcc gtatctgtcc
     4981 acgttcctgg gctttatcgg cattaccgat gtgaaatttg tcttcgccga agggatcgca
     5041 tacggtccgg aaatggcagc gaaagcgcag tctgacgcga aagcagccat cgacagtatt
     5101 gttgctgcat aaccggacac gatcttgatg tggactgtag ttcagtccac atctcaatcc
     5161 acttacctgt tttcccataa tttcaacatc gctttgtgct cttccttaag cacaatatgc
     5221 gagcgtctgg ctcgaataaa gctaatcgct tcatcaactg ttttacagtg tccgtaacat
     5281 aacagccatg ccgccactac cagcgcactg cgcgataatc ccaatgcgca atggaccaga
     5341 acgctgcctt gctcttcgcg taatgtttcc agcatcgcca cggcctgtcg gagctccccc
     5401 tcttccggaa ccaccagatc cagcatcggt acacagaaat agagtcgatc ttttgtcgct
     5461 cgtccgcgag ggaattcaaa ggtgacatcc agaaccgcat tctgtgccgg aatatgtcgt
     5521 ggaaacgccc ctaaataaac accagcagtc attttgctca ccggctccag gcgacgacaa
     5581 aaccaacgca tagatagcca catcccgatg cgccagggca atgtcagcca gtaaacggcg
     5641 ggcgggagtt tcccctgact atctttccct gttgttatcg cgccaagccc accgtaccca
     5701 cggccaataa tgagtagcga taatactggc caacataacc agactgacca ccataactga
     5761 accatcatca ttagctccat caacacaatg cacgcgcacg cgcctacgac atatggcagt
     5821 gctattttga ttcgacgttg atcaggtttc tgataattcc aacgacggtc gacgggtatc
     5881 atccagtcaa tcaacatacc taccgccagc cctgtgatga catcaataaa atgatgctgc
     5941 caggttgtca gcatcgaaat ggcgatgagt aaaaaccatc cgccgcagac tttacgccac
     6001 ctcacagcca gatgctgacg aaagtgacgc caaagtagcc agcagagaac aatatgcagc
     6061 gaaggagact ggttataagg cagatcaaac agttcaagtt gcgaaaatag ccatcctgtc
     6121 accccactca cttcaggacg gataaaacta aacttcagcg ggtagagaaa aaaaccgcag
     6181 caggccatta ccgttgccag aataagccgg tggacaagtc ggcgctgttc gaatgtcgag
     6241 ctacaaacga acagcgaaaa tccatataaa agatccagac tccagtaagg gacaatagtc
     6301 cagggaagaa aagggatcgc cgtttcccag ccgaatacct gactggggat atcatggctg
     6361 ttaaagtcct gaaccgcggt gaactgattt agagatccat aggtgaagaa gaaaaacggg
     6421 gccagcaaca ataaccagcc agcgccttgt agcaatacgt tccggcgttc agttatcacg
     6481 acgcaccgcc atcgaaaccg taaaaatgcc ccactcatca atccgttgtg tgcatttatc
     6541 aaatccggca tcacgcacga gtgaatccat ctccccttgc gaacgcacgc gcattaccca
     6601 cggtttacca tctttatgac tggttaacac cccggcaatc agctccagtt gtgggtgcca
     6661 cggctgcccg gtgtagagga gaatgccgcc cggttcgatg gcattggcaa gacctgcgag
     6721 tgagtttttt acctgctcat ttccgggaaa aagctcatac agtccagaga caatcgccag
     6781 cgtaggccgc ggagttaacg cgctgagttc ctccgggtta aacgcatcgc cctgttcaaa
     6841 acgcacccgc ccagacattc cccgttgagc aatcatctct tgcccctgtg caacatttaa
     6901 ctcgctgtaa tcacgtaaca aaatatcgct tacggcaggc tcgtttgcca gcgcatccag
     6961 tacatagcgc ccatgccctg cggcaatgtc aaccacgcgg acggctaaac ctttggcgtg
     7021 gagatcggca acggcttgtt taatcagtat ttgtaaatgg gttttgcgct ggcgaatacc
     7081 gcgccagcca acactgttca ggtagatttt gtcgattaag cgcccgaatg cgttactacc
     7141 ttgcggttga ttttgataga cataatccag cgaactgcct gaatcaaagc cggtgctcat
     7201 tccgagatgc aggcccgcag agtgcgtccc gaacaatttc atcgctttac gcataaagcg
     7261 atacgccaaa tcaaccggcg ataatggcac gggtccacct gaaaggagcc gccagcgatc
     7321 cgctgatggt ccagtgcggt cttcatgctg ataatcaaat ttttgtgact tgttagcata
     7381 taaacgacta ataaagcttt gcattttttc aaatgcctgc gccctgttct cttcacccaa
     7441 cgtgtcgtga taaaagcctg gcagcagatg cagctctttc agagggctac gtaatctatg
     7501 ataaaaatca atctgcggtt ggcgatgcac cacatagtca tcgcctgata tcagaagttg
     7561 cgtggggagc gtaatcgccg ccgcatcgct aacaatacgt tcagacgttt tataaagatc
     7621 gagcaagata ttaacggcaa tcgcccgtgt gatcagcgga tcattattga aactcgccac
     7681 ccgttgccga tcgtgggtca aatagcgtcc tttcacatag gaattaataa aaaacagacc
     7741 acgcagacga tgccataacg ccagcgcagg acgtgccagc ggcacataca atttaacctt
     7801 aaaggccgga gaagccagca ccagcccgcg aattgcaggc gcataatcat gaacccatgt
     7861 ggcaaccagc actgcgccga cgctctgcgc gatcactacc acctcttcca gcccgacctg
     7921 gctgtcgctg gcagcaaaac ggacaaactc atcgacatcc cgcactgaac gcgcaagaga
     7981 tgggctataa ccacggggcc ccgaagtttg tccatgccct cgggcatccc atgcataaaa
     8041 agcagtatct ggcatcgcca gttcatcaac gatatgttgt agacgaccag aatgttcatg
     8101 accgcgatga aataagacga tgaccttttt cgcccctggc tgtaaagcgg gccagtgccg
     8161 ataaaacaac gctgtattat cactggtggt aaaaaaatgt tccccaggga tgcgtgaatt
     8221 ttccattttc tctattttcc ttcagtaaca gcagtagcga ataaaataaa aaaataccgg
     8281 agcggtgaaa attaatgagt caatcctgtc aaggagtccg ccatgtcctg gtaatagttt
     8341 tccgctatct ttaacaccca catctcgttt gacggctgac atcactacat cgccgcaaaa
     8401 accactaata ccaattaaca aacccgctaa taatgcctgt aatgtattta gcggtgtcag
     8461 caacggtcca ataatcagtg acgcaatcat ggtggtgatt acgcccccca ccagaccttc
     8521 cagcgttttt cccgggctga ctttggggac cacttttctt ctgccgcagg atttccccca
     8581 taaatactgt gcaatatcgt ttgactctgt taacgccaaa agaaataaca ccagtaacgc
     8641 cccgccctgg gtatttgtgg ttgtcaacat taataaccag gcggcatgac tcaaggcgaa
     8701 aacggtcatt atccagcccc aaaaaatggc gctcacggta tgcagaaaac cggaggtatc
     8761 acctactaaa actcgccagg tggttaatat cagaaaccca gccaaaggta taaataacag
     8821 aaacagctca aagcaactaa aaccgataag caaatagtta agaggaataa cccaatataa
     8881 ccaacgcgga aaatgtacgg atataagcgt acaatattct ttcaatgcca gaaaacttat
     8941 cagcgcaaaa aacgtcaacg tcatccagcg tggacctgaa atcaccagtg aaaaacaaat
     9001 gacgataccc caccaggtac gaatacgtag tgtaacttca tccccttttc tctccggtaa
     9061 ccgccatacc cgaaaacggt ttatcagcgt tgctaaaatt aacaacgcga aaagtgttgc
     9121 cagagatttt tccagcatta tctttcagca ctcatcagga gcacactccg acaacgatta
     9181 atcgcagtcc agagaagcag aattgaagca atgctccata aaagattatt ccattgcatc
     9241 cactgtggat aaatggcaac ggccagcccc cacaacccaa atattaacgc gcgatcgctt
     9301 ttgccaaaag gaccgacata actacgaata ccgttaatcg tctgcgcgag taaaccgcaa
     9361 aactcagtca atatggtgca aaataacatg agtattacga gtgatgcgtt actttccggt
     9421 aaaaataaaa acggtaaata gagcgcaata tcggaaataa catcgccagt ttcatttaaa
     9481 atcgccccca gacgtgtttg ctgattgcac tcacgcgcca acatgccatc cagcgcattg
     9541 agtgccatac ggataaaaag cacgatgggc aatagcaaaa agaggatggg ttgtgcgacc
     9601 aacatcaaca gcaatccggt aataagagaa agcgccagtg cagcaagagt gatgtgattc
     9661 gctgtaacat ggtgcttata aagccaaaac atcgtcggcc ttaacagcga ctgaaagagc
     9721 ggttttatct ggtagagcgt cattatcaat ccttgataat tcggtttgcg ctatggtgca
     9781 tcaattcccc tgacggagat atcgcaacac gacgcttcat taaaaattca gaatatatca
     9841 aggtattggg gactcactgt caggaatgat tatccccaat aaatcaagtg gttaaaaatt
     9901 attacgctgt tattcaatac aactcatcaa gttttctcat cgcttgttcc cgtattgcgc
     9961 gcgtcttacc gttaagcgcc agctggcgaa ctaaatccag ttcagggtta acaagcttta
    10021 acgccagcaa aggatcacgt ttcagcctcg cctttaatac atcaactgga aaacggggat
    10081 tattcatcgc cacaatccac cactcgggat caatttctgc cgccagtact gcaggcggtg
    10141 tgtggcggtt gttaagtgcg ccgaacttga aaaatgtgag attgcttttc gccagtgtac
    10201 tccaccatat acgatccggt tgttttgcca gtacatccag ttgtgctaac gcatcggggt
    10261 ctagtgcata ataaacacgt aacgcttcgt taacctgcgc acgggaactg ttttcctgcc
    10321 tcagaaaaaa cttcgcccaa atatcattgc gtggcgtgtt ttgctgtaat tccttgagaa
    10381 caacagcggg taaattatcc caactaatta atgcactctc taaaaagtcc cagtcgtccg
    10441 aacgtatcag agaggtggca tttaatagca tctggtcatc aacttcaccg tagctgataa
    10501 gctcagacaa aatcatatca gcgctggttt catatgcatc tgaatctgct ttgggattat
    10561 ttttccgatg cgataaagca cgttgccaaa ttttttgttt catcgacgtg ctcagatact
    10621 gatttttcgc cagattactc cacccctcag gatcgttgaa acgtgtcaat aaccgttgta
    10681 aagcagcgtc agagtgaatt atctgcatat cccgacagca aaagtaggca ggggattttt
    10741 tctccatcct tttaacaaaa ttaaactgta agttatctgg caacgctaaa aataacgcac
    10801 ctattagatc atcgtcattc tcgaattttt ggtataaata tactgctatt ttgtcactct
    10861 cttgtggcgt catccgcaac aattgaccat ggtgtttcat ctctgccagc tttaacgcta
    10921 agcttgtgac aacatcgttt gatgaatcgt tcgccaactg cattgcctga ttgtaggtga
    10981 gttgtcctcc agcaccgacc tgacgaatag aggaatcatc actgcggata tagccatcaa
    11041 taatggattc gggcaataac cacgcatctt tataaagctc ctgaatgatc atatcccaat
    11101 ctcttttcgc cagagtcatg acgatatcca gagggagatt ttcacgtagc gcaagattca
    11161 acggaccgac accatcaggt agatgactca ttcgcgtcat tatttcgaca gagagcggtt
    11221 cacggtcgag caaccccaga acaacaggct ggtaacccaa ttgctgatcg gtcaactcca
    11281 gaatctcatt gtcaggcata tatctcgcca tactttcaat cgtttgtcca tcattgctat
    11341 taataagttg tttgcgtagg ttagctgaaa ttcctttaag ccttgggaga gttcgcatta
    11401 tggcaagatc aaatgtcgca atatctcgtc cttcaaatgc agcaacgttg ccttcatcca
    11461 gcatttgcaa ttcctgcact aactcaggag aaggcttaag gcgaaacagc gcttcttttt
    11521 tcatctgatt acagacattc cctgccatgc gaatattgct tcgcttaaaa atttctttca
    11581 gagcatcttc atcatttatt agaccccaat aatatgcatc aggatttatt atcatcagcg
    11641 cttttttgac attttcagtg tcatttttgt cactcgcacg gaccatgaat tcactatttg
    11701 tcgaagacgc aacaacaggt gcaattatgt cattgataaa tatcccacgc gcattaatac
    11761 tccccttaat cgtagacgga ttatcgtaat atgagtcgat aaataccagt ccacttgcat
    11821 ttatcgaacc ggtaattaac agtggtgaat ccgtaactat attcgttgcc gtcacgttac
    11881 cgagaataac ccatggatta gaaagagaga aagtggaaat attacctttg actgtcaaat
    11941 tgccaagaac aatcaatttt tgattactct ctatattacc ttctaccact aaatcgccac
    12001 gatgcacttt ccagtccgat gcagataaac cgaaggggac aagattcacg ctactgttga
    12061 tagcttgttc aacttgttgt ttatcttcag cggtagccgc agaaaaaaaa ggttcaatat
    12121 caactgcatt tgcaacgcat cgaagttccg cctgtactgg aagactcagc ataaacaggg
    12181 gaagtaaacc tatccagctg caaagcctgg gcttaaacat atgcactcca tgccattaaa
    12241 aaagaaacaa aagggaaatg catctttccc ttttgtatat tcgttattct cgcgaaatac
    12301 catccaacgt agacaacact ttctcagaaa gatgcaactt ttcagcagcc atattctccc
    12361 gtaaatgcgc aacagaagac gtccctggga tcagcaaaat attcggtgaa cgttgtaaca
    12421 gccacgccag cgccacctgc attggtgttg cacccaagct cgcagcaaca tcggaaagcg
    12481 tggaagattg cagcggcgta aagcccccga gcggaaagaa cggcacgtag gcaatgccgt
    12541 cacgagccaa agcttcaatc attgcgtcat cagcacgatg agcaatgttg tattcgttct
    12601 gcacacagac gatttcggca atcttgcgcg cctctgcaac ctgcgtcggt gtgacgttgc
    12661 ttaggccaat atgttttacc aggccttgtt gttgcatctc tgccagcacg gtcaggctgg
    12721 cttcaatcga tccttccgcc gggccatgac catcccccat catcacgcgc aggttaacca
    12781 catccagtac gtccaggccg agattacgta gattatcgtg caccgctttt tgcagctctg
    12841 ccggagaaaa tgcgggcaac caggatgcgt cctctccacg ccgcgcacca attttagtga
    12901 caattgtcag atcgtcagag taaggataaa gcgcttcgcg gataatctga ttggtgacgt
    12961 gcggaccata aaagtcgctg gtatcaatgt gattgacgcc caatgccagc gcctcacgca
    13021 gcacggttat agcgacgtga cgatctcttg gggggccaaa aaccccagga cctgccagtt
    13081 gcatcgcgcc ataaccaaga cggttaacgg attttgtacc gagagtaaat gtattgctgc
    13141 tcatcgcacc ctgccctgta ataaaacgga ttaagaaaaa tcagtataga aacgaaaaga
    13201 gataaataat gcgaaggcgt aaacaagatt tctggatgag tagcactatt gtcctgaaag
    13261 acaccaaaac attagacagc aaaagataag tcaagcgtcg catccggcaa taaaaaaccg
    13321 caacctgtat cagcgttgcg gtttgtgtat cacatgctaa aaattagaac gttttcttaa
    13381 agttcagcat cacgcctttg tcgctgatgc cgtcctcttt ccagtgatac gcatccagat
    13441 tcagcgaact ttctttgcta ctgtacttca cgccgacgct cgccaggctg ttgataccgc
    13501 cgcccttctg accgtcatcg acgttaaagt gctggctgcc tgcccctgca aggcttgccg
    13561 tgcgctggct cttgctgtag ctcaggttcg gcccgccttc cagcgtcgca ttcgcgctcc
    13621 agccgccttt ccctgcgtag tccagtttca gcccgacgat gctgtccacc gccgtttcgc
    13681 tgccgctgtt catgctcagg ttaaagtctc cggcattgcg ctcctgatag ccgccttcca
    13741 gcgagtgacg cagtttcact ccggcgtacg gggtgatttt cagcccttcg cgcggctcaa
    13801 aggttttcgc cccttcgcta cgcaactcca ggtactgctg tttcacatcc gtatccgccg
    13861 ttttgctgac gtcgccgtaa gcgaccgagc ggctgctgtc gagattatgc acgtcataac
    13921 gcagcgcgtt gttccaacgc ataccattgt cgaaggacat ctggtgcttc aggccaaaga
    13981 actggctgta gccgccggtt acgccattgt cgcccgcttt ctgcgcaccg tcgccatcca
    14041 gacgcgcgat accgtattcc aggctcatgc tctggctttc gctcaggtca acggttttac
    14101 gcagtgccag catgtcatac tcggtgttat ttccgagttc cgcacgcggg tcacctttcg
    14161 ccaccacgtt aaaggccagg ccattgccca ccttcggcgc ggcatccgcc agcatgctga
    14221 agcggttgct taacacacgc gcttcacgga ataccgtggt cgcctggcta ccgctcacct
    14281 gcttcagggc gctattcagt tcagcagtgg tgcccacgtt caggctggta tacagctcgt
    14341 tattggtgta acccgcatcc agtgccttcg ccacatcgtt caccgaagca tcggtcgcca
    14401 catcggtgta ggcgtttttg ctcatgatga cgtcaacgtt gccgctggca tcggtgctgc
    14461 ctttggcggt ccataccacg gacgttgagg tgatggcatc ggcgtcggtc aggttgatgc
    14521 cttccaccac gttgtcgaaa ctgaccgtgg tgtctgccgt accggcggcg aaaccggtgt
    14581 tgatcccgac accattcatg ctggcgttgt tgaccttcag tttaccggcg ctgccgtcaa
    14641 cgttggtacc gacgatatag ccgttgaggt tgttactgct gccatcgcta ctaccgctat
    14701 cagtcgtttc actgaaaggt gaagtggtgt aagtgtttgg catgtccggc aacgtgtagt
    14761 cggtgtaatg cacttcggta ttgttaccgc tgttgccgtt caccccttcc acattcacgc
    14821 tgtcgccctg cttaatcaac cccgaaccag tcacgccgtc ggcaatcacc accgtaccgt
    14881 tgttaacgat atgaccttct gtgcccagta cgctgaacgc aaacgagtcg ttagcaaaaa
    14941 tattgatcgt gccgttgttc tcaattaccg catcggcggt ggcattggca tccagttgca
    15001 taccagtcaa tccggtatcg gtggtgcctt cggtaccgag attgataacg ccgttgttaa
    15061 ccagctgtgc gtttttatcg ccttccatcg ccacggcgtt gttgccgctg atgttcatta
    15121 caccatcgtt gagagcaaca tagttgttgt gggtgaattt aaccgcgctg gcgttgtctt
    15181 tatcgaagtt aattaccccg ccatcctggt tccacaagaa ggcatcgctt tctgtcgcac
    15241 cggcagtact taccgccacg gtgactttcg catccaccac gcccttgtta ataaactcgc
    15301 tggcgccatc gtcgtagtga atagcgcccg cacctaatgc ggcatccagc gtcatttcac
    15361 cggtgttata taacgtggcg ttagcattgc tgccctggaa gcgaatggcg tttttgctgt
    15421 tatcaaggat gagtttgccg ctgttgttga acgttgactt atcgtcaata tccagcgtcc
    15481 cggcattgtt gttttcatcc tgataaccca ttttccaggt acccgcgtta ttcactaccg
    15541 cagagttata gagaatcgcg cctttacgcg cctcgccgcc tgcaaggtta ttgaataccg
    15601 catcgccctg agcgttaacg cgcgccgacg caataccgct gttattcacc gtaccatcgt
    15661 gaagttgtaa agttgacagg gaaccggtgt tattaattac cgcgttgctg tttttctcaa
    15721 tttttacgac gtcattaagc gttccgttgt tattgaaggt tcctccttcc agagtcaccg
    15781 tgttcaatac accttcattg gttaacgcac cgccttcatt cacgacgatg gctgagctaa
    15841 tggttccgct cgactcgtta accagcgcac cattttcgag tatcctgatc gttccatttt
    15901 taacagcacc cgcactggtg acatccccgt tgataatcgc acctgacgat aataatacgg
    15961 tctcacccgc agaacttaat acagcgccat catcatataa atcagcaata taattatccg
    16021 ttggattata ttcatcggcc ccctagcaaa cgccaaaggt acaaattgtg ccatagttga
    16081 taataagccc agtgccatcg ttatagaatg cctgaccatt ttccgcgtta atgttgatta
    16141 cgcctgtctg gtcattaatg gcggtgcctt cgtgataaac cgccatgccg acgagtttat
    16201 tcacaccgag agaatcgttg taattttcat ttttctccag gttgatggtt ccctggttaa
    16261 tgaccgtatt agtattccca tacgccgcca tccccgcacc ggcgttgtag acagtaatca
    16321 caccattctc gtggttggtt gccgtggctt tagttaatgc cccattatag gcatcggtac
    16381 ctgaagccat tcccacaccg aaatctttcg ccgtgctatt tgcaacatta ttacgcagcg
    16441 atgtttcatc attttcatca acccacagcg tatccagggt gatttgccct tcgttatccg
    16501 cggttgattt atcgatggct agcatcccta cactgccacg gccatacata tcaataatac
    16561 ccgtttcgcc gttcagaatg gatgacgagt agctggcaag catacctgtc agccctaatg
    16621 ccttgttatt gaaagaggta ccatcagaca agttccactc tgccggataa ttcagcacac
    16681 tgtgctgatt catcattttg gtggtcatgg taccattgtt aacgccaatc ccaccgtatt
    16741 caacatcaaa gacttttggt gccgggtcgg taccaaaatc gtacatcgcc aggccaatgg
    16801 atccgttatt gattaccgtt gaatctttat tccaggcagc cgcaaaaccg atatttttca
    16861 gttccacttc accgttgttt gtcgccttcg caccagaatc aatactaatg gtggataacg
    16921 ctacacgata ataataagtg aaagaatcca catcactcat atcaaacgtt gcgtcagcgc
    16981 caatctccag ctccgcgcca tggctcaact gaatgagatt ttcgaacccc agcgggaact
    17041 cgccgctgat ggttgactca ccattcaggt agaccgggga atgcccatca acgttaataa
    17101 gcggtgtgtc tccgatacca cgtcgagaag ctgatggttt tgagccatca tccatgacat
    17161 ttttttcacc gacaaaatgg acaccgccat taagcgtaaa ggtattgtca tcgccagcaa
    17221 taacaatacc tgcaatgtgt tcctgagcac cgtcgtaact cgcataggaa cgcgacgtgg
    17281 ttttactatc agcaacaacg gttaactgac catccagcgt gacgtcgttg ttgtcaccgc
    17341 tgatatttac cccggttgag ggattaaaga agtaaggctg cgcattatcg gctttctgat
    17401 ctttatcaac cagaatactg cccgctattt cgatagtatt accatcaccc gaaacattaa
    17461 cacccgtcgc tttttgccct gtcacattca gctcataagt cgccagcgtc atgttgttat
    17521 tattgccaga gacatcaaga ccggtggaga aatccccaac gtctaaccca cccgcgagtg
    17581 aaatattagc tttatcccca gcaacgctga caccggttcc ggcattggtc gcggaaatat
    17641 caccaacgtt aacaaatgtc gcattatcac cgtcgatctg aatgccaatg gcattttcac
    17701 ccgctgcggc ctgaccaaca ttgaggtcgc ccatgttgat cacgctgacg ccatcaccat
    17761 caagcagcat gccaatagag ttatcttcga tgaccgtaat attacctttg ttgctaacga
    17821 acgtatcatt gccgtcaacg tcaataccgt gcgcaccacc gcttacgtat aagtcgcctt
    17881 cctgtttgat ggtggcaccg tcaccgacaa ttttcgagcc agtggaattc tctccgtcaa
    17941 cgctcatggt accttgatta tcgacccgcg cattatcgcc attgataagg ctacccgtac
    18001 caccattggt gatggtcgag tcgcccgcag tgtttagctt ggtattatcg ccattgatga
    18061 ttaccgcggt accgccatca acttccgtct cgccattatt gttaatggtg atattgtcgc
    18121 cagtgagtat accgacaacc gagccttctc cgatggccgt cgttttgccg tcgttgttga
    18181 gggagatgtt gtcaccatca accgtcatga cagtaccgcc attcagcgcc gtgttgccgc
    18241 cctgatcggt gatgatcgcg ttaccaaact caatcgcatt cccggttgtg ccgtcaacaa
    18301 cgacgccggc ctctttagtg atgtggatga gtccatcatc gtcgtaactc caggtgatgc
    18361 cacctgaggt atttacgcca tcaaagacaa cggtgttgct gtcgtcatgg actgtccagg
    18421 acttaactat cgtctccttg ccctcaggcg ccgttaaggt gtagctccca tcggcatttt
    18481 ggctgtaggt aaaggtagtt tcgcgaattt tgacagtttt tgcatcctga tcccaagtta
    18541 cgccattttt atagacgaca ggattgtgtt taaccggggt atcgccacca tcgtctggcg
    18601 gaactggcgt atcaccgccg tcatctggcg gaaccggcgt atcaccgccg tcatcaggcg
    18661 gaactggcgt atcaccgccg tcgtctggcg gaacaggcgt atcaccgcca tcatccggaa
    18721 ctggcgtatc accgccgtcg tctggcggaa caggcgtatc accgccatca tccggaactg
    18781 gggagttatt atggtggtga ttgtcatcgt cacttaaggt atatatagca attcccgtag
    18841 ctactgcagc aagacctgca gctgcccatg gtaaaactgc actatcttta ttttctctgc
    18901 actccgcgga taaattttct agctgctctt tgctcaggga atgaatattt gccggacatg
    18961 ttatcttact ggacttacgt gttttttcgg ttgagtcggg tattgaagta tccgcagccc
    19021 atgtctggct acttaacgcc atggcaacac aaacagacaa taattttttc ctttgcata
//
LOCUS       DAZFDC010000091        18735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFDC010000091 DAZFDC010000000
VERSION     DAZFDC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.091"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(23..211)
                     /gene="bcsR"
                     /locus_tag="ACHJ48_003283"
     CDS             complement(23..211)
                     /gene="bcsR"
                     /locus_tag="ACHJ48_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO4708774.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPGIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            484..2055
                     /gene="bcsE"
                     /locus_tag="ACHJ48_003284"
     CDS             484..2055
                     /gene="bcsE"
                     /locus_tag="ACHJ48_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO4708775.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            2052..2243
                     /gene="bcsF"
                     /locus_tag="ACHJ48_003285"
     CDS             2052..2243
                     /gene="bcsF"
                     /locus_tag="ACHJ48_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO4708776.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            2240..3919
                     /gene="bcsG"
                     /locus_tag="ACHJ48_003286"
     CDS             2240..3919
                     /gene="bcsG"
                     /locus_tag="ACHJ48_003286"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO4708777.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(4006..4113)
                     /gene="ldrD"
                     /locus_tag="ACHJ48_003287"
     CDS             complement(4006..4113)
                     /gene="ldrD"
                     /locus_tag="ACHJ48_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4708778.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            4589..5860
                     /gene="yhjV"
                     /locus_tag="ACHJ48_003288"
     CDS             4589..5860
                     /gene="yhjV"
                     /locus_tag="ACHJ48_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO4708779.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFTMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKTWLILLYGILLCLSPLLKLIE"
     gene            complement(5890..6894)
                     /gene="dppF"
                     /locus_tag="ACHJ48_003289"
     CDS             complement(5890..6894)
                     /gene="dppF"
                     /locus_tag="ACHJ48_003289"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO4708780.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKIGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(6891..7874)
                     /gene="dppD"
                     /locus_tag="ACHJ48_003290"
     CDS             complement(6891..7874)
                     /gene="dppD"
                     /locus_tag="ACHJ48_003290"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO4708781.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(7885..8787)
                     /gene="dppC"
                     /locus_tag="ACHJ48_003291"
     CDS             complement(7885..8787)
                     /gene="dppC"
                     /locus_tag="ACHJ48_003291"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO4708782.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(8797..9816)
                     /gene="dppB"
                     /locus_tag="ACHJ48_003292"
     CDS             complement(8797..9816)
                     /gene="dppB"
                     /locus_tag="ACHJ48_003292"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO4708783.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(9967..11574)
                     /gene="dppA"
                     /locus_tag="ACHJ48_003293"
     CDS             complement(9967..11574)
                     /gene="dppA"
                     /locus_tag="ACHJ48_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO4708784.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(12319..12395)
                     /locus_tag="ACHJ48_003294"
     tRNA            complement(12319..12395)
                     /locus_tag="ACHJ48_003294"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12359..12361),aa:Pro,seq:cgg)
     gene            complement(12487..14178)
                     /gene="eptB"
                     /locus_tag="ACHJ48_003295"
     CDS             complement(12487..14178)
                     /gene="eptB"
                     /locus_tag="ACHJ48_003295"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO4708785.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            complement(14433..14963)
                     /gene="lpfE"
                     /locus_tag="ACHJ48_003296"
     CDS             complement(14433..14963)
                     /gene="lpfE"
                     /locus_tag="ACHJ48_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO4708786.1"
                     /translation="MKFKRLLHSGIASLSLVACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            complement(14968..16023)
                     /locus_tag="ACHJ48_003297"
     CDS             complement(14968..16023)
                     /locus_tag="ACHJ48_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4708787.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRTV
                     DSMKYYTLTEELEVATNIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTVTQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLSFDCNNISDGIKVALRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDSSSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            complement(16038..18611)
                     /gene="lpfC"
                     /locus_tag="ACHJ48_003298"
                     /pseudo
     CDS             complement(16038..18611)
                     /gene="lpfC"
                     /locus_tag="ACHJ48_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
     gene            complement(18640..>18735)
                     /locus_tag="ACHJ48_003299"
     CDS             complement(18640..>18735)
                     /locus_tag="ACHJ48_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="binds with fimbrial subunit LpfA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB"
                     /protein_id="HFO4708788.1"
                     /translation="LPAGVGSVSKIKYNTINDFGSSAEMITKNVD"
BASE COUNT         4497 a         4981 c         4602 g         4655 t
ORIGIN      
        1 agtacggcca ttcaccaatc ccctactttt gttgcgcaaa ctctgccagc aacggccagc
       61 gttttaatgc cgcggccaac tgttcgcgtt gggaaatatc ggcataatca ataccaggca
      121 gtgaaaatgc ctgctttaac gccaaaatat cattctggaa gatatagcct atcgcgggat
      181 caggcagagt atctggttca ttgttattca ttttgcttga tccctatgaa ataaccgtac
      241 cgttcatgag acaattctaa ccgtgagcat ttcgccggtt tattgtttac ggcgagacgg
      301 gaatttgctc actttttgac tttccgttac gcaacagaaa aagttaaaaa cgaaaagact
      361 aaaatcacac gtcttgtgac ctgctaaatt gattcacatg ctaaatctga taagttttaa
      421 tttcaatggt aggtttattt cttagctttc gctagtaaac tgataatcag acaaaatggt
      481 gacatgaggg acattgtgga ccctgtattc tctatcggta tctcatcatt atgggatgag
      541 ctgcgacata tgccagcagg cggcgtctgg tggtttaacg tcgatcgcca tgaagatgct
      601 atcagtctgg cgaatcaaac aattgcatcc caggctgaaa ccgcacacgt cgcggtcatt
      661 agcatggaca gcgatccggc gaaaatcttt caattagatg attctcaagg gccggaaaaa
      721 ataaaattat tttcaatgct aaatcatgaa aaaggtctat actatttggc ccgtgatttg
      781 cagtgttcta ttgatcccca taattacctt tttattcttg tttgcgcaaa taacgcatgg
      841 caaaacattc ctgccgagcg gctgcgctca tggttggata aaatgaataa atggagcagg
      901 ttaaaccatt gttcgctttt ggtaattaat cccggaaata ataacgataa acaattttca
      961 ttgttgcttg aggaataccg ttcacttttt ggtcttgcca gtttgcgttt tcagggcgac
     1021 caacatttgc tggatattgc cttctggtgc aacgaaaaag gggtcagcgc ccgtcagcag
     1081 cttagcgttc agcaacaaaa tggtatctgg acattagttc aaagcgaaga ggcggagatc
     1141 caaccacgca gcgacgaaaa acgcattctg agtaatgttg ctgtactgga aggtgcgccg
     1201 ccgctatcgg aacactggca actgttcaac aataacgaag tcctgttcaa tgaagcccgt
     1261 accgctcagg cggcgacggt ggtcttttct ttacagcaaa atgcgcaaat cgagccactg
     1321 gcccgcagca ttcataccct gcgtcgccag cgcggtagtg cgatgaaaat cctcgtgcgg
     1381 gaaaataccg ctagcctgcg cgccaccgat gaacgtttgt tattggcctg cggtgcaaat
     1441 atggttattc cgtggaatgc gccactctcc cgttgtctga cgatgatcga aagcgtgcaa
     1501 gggcagaagt ttagtcgcta tgtgccggaa gatatcacta ccttgctgtc aatgacccag
     1561 ccgctcaaac tgcgtggttt ccagaagtgg gatgtgttct gtaatgccgt caacaacatg
     1621 atgaataacc ctctattacc tgcccacggt aaaggcgttc tggttgccct acgtccggta
     1681 ccgggtatcc gcgttgaaca agccctgacg ctgtgtcgcc ctaaccgtac cggcgatatc
     1741 atgaccattg gcggtaatcg gctggtgctg tttctctcat tctgtcggat taacgatctg
     1801 gataccgcgt tgaatcatat tttcccattg cctactggcg acattttctc aaaccgtatg
     1861 gtctggtttg aagatgatca aatcagtgcc gagctggtgc agatgcgcct gcttgcccca
     1921 gaacaatggg gcatgccgct gcctttaacg caaagttcta aaccggtcat caatgccgag
     1981 cacgatggtc gccactggcg acgaatacca gaacccatgc gactgttaga tgatgctgtg
     2041 gagcgctcat catgatgacc atcagcgata tcattgaaat tattgtcgtt tgcgcactga
     2101 tatttttccc gctgggctat ctggcgcggc actctttgcg acgcattcgc gacaccttac
     2161 gtttgttctt tgctaaacct cgttatgtta aaccggctgg gacgttacgc cgcacggaaa
     2221 aagccagggc aaccaaaaaa tgactcaatt tacgcaaaat accgccatgc cttcttccct
     2281 ctggcaatac tggcgcggcc tttccggctg gaacttctat tttctggtta agttcggcct
     2341 gttgtgggcg ggatatctta acttccatcc gctcctcaat ttggtgtttg ccgcgtttct
     2401 gctgatgccc attccgcgct acagcctgca tcgcttgcgc cactggattg ccctgccgat
     2461 cggctttgct ttgttctggc atgacacctg gttgcctggc ccggaaagca taatgagcca
     2521 gggttcgcag gtggcggggt tcagtaccga ttatttaatc gaccttgtca cacgctttat
     2581 taactggcag atgattgggg ccatttttgt tttattagtg gcctggttat tcctgtcaca
     2641 atggattcgc attaccgttt ttgtggttgc catactgcta tggctgaacg tacttaccct
     2701 ggcgggacca agtttctcct tgtggccagc cggacaaccg acgaccactg taacaacgac
     2761 gggtggtaac gcagcggcaa ccgttgcggc gacgggtggc gcaccggtag tgggtgatat
     2821 gcccgcacaa actgcaccgc caacaacggc gaaccttaac gcctggctga ataatttcta
     2881 taacgcggag gcgaaacgta aatcgacctt cccgtcttcg ctgcccgctg atgctcagcc
     2941 atttgaacta ctggtgatta acatctgttc gctttcctgg tcggatatag aagccgctgg
     3001 gttgatgtcg catccactgt ggtcgcattt cgatattgag ttcaagaact ttaactccgc
     3061 cacctcctac agtggcccgg cggcgatccg tttactgcgc gccagctgcg ggcagacttc
     3121 gcacactaat ctgtatcaac cggcaaataa cgactgctat ctgtttgata acctgtcgaa
     3181 actgggcttt acccagcacc tgatgatggg acataacggc cagttcggcg gttttttgaa
     3241 agaagttcgc gaaaatggcg gcatgcagac tgaattgatg gatcaaacaa atctgccggt
     3301 tattttgctg ggctttgatg gttcgccggt ttatgacgat accgccgtgc ttaaccgctg
     3361 gctggacgtt accgaaaaag ataaaaacag ccgtagtgcc acgttctaca acacgcttcc
     3421 actgcatgac ggcaaccatt atccgggcgt cagcaaaaca gcggattaca aagcgcgggc
     3481 gcagaaattc tttgatgaac tggacgcctt ctttactgaa ctggagaaat cgggtcgtaa
     3541 agtgatggtg gtcgtggtgc cggaacacgg cggcgcgctg aagggcgaca gaatgcaggt
     3601 atctggccta cgtgatatcc ctagcccgtc tatcaccgac gtccccgttg gggtgaaatt
     3661 cttcggcatg aaggcaccac atcagggggc accgattgtc atcgaccaac cgagcagctt
     3721 cctggctatc tccgatctgg tggttcgcgt tcttgatggc aagattttca ccgaagacaa
     3781 tgttgactgg aaaaaactca ccagtgggtt gccacaaaca gcaccggtct cagagaactc
     3841 aaatgcagta gttattcaat accaggataa accgtacgtt cgcctgaacg gcggcgactg
     3901 ggtgccttac ccgcagtaag cacgaaaaag gccgcagagt ttcccccctg cggtctggtc
     3961 cgggcgcaaa ttgccattac ggcagcctga cgcccgcatg acacgttact tccgcttgtt
     4021 cagccagttc acgatcatac tggcaagaat gccagcaatg accggagccg ctaaatcatg
     4081 ccagaaggcc atgcccagct ctgcgagcgt catatagccg cctgtgttgt aatgacaacg
     4141 tttcgcggct attcttgagt ggtctaggtt caagattagc ccccgtggtg ttgtcaggtg
     4201 catacctgca acgtgcgggg gttttctctc tccagcaacc aatgccacca gggattaagc
     4261 ccccgcaaca ttgcgcctca ccggaaccct tccggcttgc cgctaatttt acgattgctt
     4321 tttactgtca gcactcggca atcttcctca ttgcgtgatg ccttccagct taatttctta
     4381 atttgcaata gttgcagttc atatccgggc tgactccaga ctggtagtga aatgcgcaat
     4441 tttgtagaat tgcgcctcct cgcgacctcc ccagtgaatc tgttgcctca ttttttacat
     4501 actgcgtatt cgacttctcc acctgttgcg caagagaaac tgggtttatt catttttgcg
     4561 aggccgactt ctttctggac aggactttat gcagcacaac acactaccga aacacgatca
     4621 gaaattgccg tttacacgct acgacttcgg ctgggtttta ttatgcatag gcatggcgat
     4681 tggtgccgga accgtgctga tgccagtaca aattggcttg aagggcattt gggtatttat
     4741 taccgcagcg atcattgctt atcctgccac ctgggtagtg caggacattt atttaaaaac
     4801 cctttctgaa agcgattcct gtaatgacta caccgatatt atcagtcatt acctggggaa
     4861 gaactgggga attttcctcg gggttatcta ctttttgatg attatccacg ggatttttat
     4921 ctactctctc tccgtggttt tcgacagcgc ctcgtacctg aaaaccttcg gtttaaccga
     4981 tgccgatctt tcacaatctc tactttataa agtcgctatt ttcgccgtac tggtagcgat
     5041 tgcgtctggt ggtgaacgat tactgtttaa gatttctggg ccaatggtgg tggtcaaagt
     5101 agggattatt gtcgtgttcg gttttacgat gatcccgcac tggaatttcg ccaatataac
     5161 cgccttcccg caagcctccg tcttttttcg cgatgtcctg cttaccattc cattttgctt
     5221 cttttctgca gtatttattc aggtacttaa cccaatgaat attgcctatc gtaaacggga
     5281 agcggataaa gtactggcaa cccggctcgc gctgcgtacc caccgaatta gttatatcac
     5341 gctcatcgcg gtgatactgt tttttgcctt ttcgtttacc ttctcaatta gccacgaaga
     5401 agccgtttct gcctttgaac aaaatatctc ggcactggca ctggccgcgc aggtgatccc
     5461 tgggcatatc attcatatca cctctacggt gctgaatatc tttgccgtac tgaccgcatt
     5521 ctttggcatt tatctcggtt tccacgaggc cattaaaggc attattctca atctgttaag
     5581 ccgaattatt gataccaaga aaattaactc acgcgtgctg actctggcga tctgcgcttt
     5641 tatcgtcatt acgttgacga tttgggtttc gtttcgtgta tcggtgctgg tgttctttca
     5701 gttgggaagc ccgttatatg gcattgtgtc gtgcctcatt ccgtttttcc tgatctataa
     5761 agtcgcacaa ctggaaaaac ttcgcggatt taaaacctgg ctgattctgc tttacggcat
     5821 tttgctatgc ttgtcgccac tgttgaagct gattgagtaa accggagcgc atggccccgg
     5881 ttttgtgagt taacgctgcg gattttcatc ctgatcaaca gcaaaacaag ctaccagttg
     5941 accgccgtag tcttttagct gcggctgcaa ctgggtgcag gggccgaagc gccgacgaca
     6001 gcgggcgtta aacgcgcagc ccggcggtgg attcagcggg cttggcagtt caccggtgag
     6061 tttgatgcgc tcgcggcgat cgtccggatt caggcgcggc gtcgcggaaa gtagcgcctg
     6121 agtgtacgga tggcgcgggt tattgaagat ttggtctttc gttcccttct ccacgcagcg
     6181 gcccaggtac atcaccatca cttcatcagc aatgtgctcc accaccgaca ggtcgtggga
     6241 gatgaagaca taagaaagcc ccagttcctg ctgcaaatcc atcatcaggt tgagcacctg
     6301 tgcacgcacc gagacgtcca gcgcggagac cggttcatcg gcaatcacca cgtccgggtc
     6361 gagcatcaga cctcgggcaa tggcgatacg ctgacgctga ccgccggaga acatatgcgg
     6421 atagcggtca tagtgctcgg ttttcaggcc gactttcgcc atcatcgaca gggctttttc
     6481 ccgacgctgt tctttgctta agctggtgtt gatcagcagc ggctcttcaa gaatttgccc
     6541 gattttttta cgcggattca gcgaaccgta agggttctgg aagacaatct ggattttctg
     6601 ccgacgcagc ttctgcgcct gcggatcgtg cttaagcaga tcctgcccct ggtaatacag
     6661 ctcgccgccg gtgggcgttt caatcatcgt cagcaaccga ccgagggtcg atttaccgca
     6721 gccagattca cctactaccg ccagcgtttt gccacgttca aggttaaacg aaacgccgtc
     6781 cagcgccttc accagacgtt ccggagcgaa catacctttc ttcaccggat aatgtttttt
     6841 caggtcgata gcctgcaaca gcggttgttg cgaggtggcc tcttgcgtac tcatagtgtc
     6901 ggcctcccgg catcatcaag tgggtaatgg catttggact gacgcccgtc agcgagcata
     6961 ttcagcgccg gttcttcagc gcgacatctg tcagtggcat aggggcagcg cgggttaagc
     7021 aagcagccgt tcgggcggtc gtacttgccg ggaacaacgc caggcaacga tgccagacgt
     7081 tctttgtcct gagcaaattc cggcagcgca cgcagcaatg cctgagtata cgggtgacgc
     7141 ggcgcatgga agatggcgtg cgcatcgccc gtttccacca cctgaccggc atacatcacg
     7201 atgattttat gtgccgcttc cgccaccagc gccaggtcat gggtaattaa caccagcgcc
     7261 atgttctctt tctgctgtag ctccagcagt agttcaatga tttgcgcctg aatggtcacg
     7321 tccagcgcag tggttggttc atcggcaatc agcagttttg gccgacaggc aatggccatc
     7381 gcaatcatca cgcgctggct catgccgccg gaaagctgat gcgggtaaac atccagacgc
     7441 gaagccggat cgggaatacc gacctgattc aacaagtcaa tcgcccgctg acgacgagtg
     7501 ctcttattac cgccctgatg caccttaatc gcttccataa tctggaaacc cacggtgtag
     7561 cacgggttaa ggctggtcat cgggtcctgg aagatcatcg ccacttcggc acccaccagg
     7621 ttgcggcgct ctttttccga gatacgctgc aaatcctgac cgttaaactc cagcttttcc
     7681 gccatcacgc ggcccggata atcaatcagc cccataatcg ccagcgaact gaccgactta
     7741 ccggagccag actcacccac aatcccgact acttcgccct gttttacgct gtagctaatg
     7801 cggtctacgg cgcggaacgg tgcgctttcg tcgccgaaat gcaccgataa tttatctaca
     7861 tttaataacg ccatctcgaa cctcttactg cttcagtttg ggatcgagcg cgtcacgcag
     7921 accgtcaccc atcaggttaa atgccagcac cgtcagcagg atcgccagac ccgggaaggt
     7981 cacgacccac caggcgcttt gcgcgaactg caacacgtcg gagagcatgg tgccccactc
     8041 cggcgttggc ggctgcgccc ccatgccgag gaaaccaaga gcagccatat cgagaatggc
     8101 gttagagaaa ccgagcgacg cctgaacaat cagcggtgca aggcagttcg ggaagatgtt
     8161 aataaacatc tggcgcatcg ccccggctcc tgccacgcga gaagcggtga cgtaatcgcg
     8221 attcacctcc accagcacgg cggcgcgggt taagcgcaca tagtgcggca aggcaacaaa
     8281 ggtcagcgcc agcgcggcgt taccaatcga cgggccgaaa atcgccacca gcaccagtgc
     8341 caacagcaga cttggcagcg ccagcatgat atcgaccacg cgcataatga tgttatcgac
     8401 caggccgccg aagtaaccgg cgatcaggcc gagaataacg cccataatca gcgataacac
     8461 caccaccaga cagccaacca gcagcgacaa gcgcgcgccg tacatcagcc gcgacagcac
     8521 atcacggcct acatcatcgg tacccagcaa gtgcgccatg ctgccgcctt cctgccaggc
     8581 tggcggggcg agcagtgcat cgcggaactg ttccgccggg ttatagggag caatccagtt
     8641 ggcaaagatc gcgataaaca gcacgatgac gacgtaaacc agcccgacga ccgcgccttt
     8701 gttgcgttta aaatagtgcc agaactcctg taaaggggtc atcggtaccg gcgcgctaat
     8761 cactttattt tcagtaacct gtgacatgat gaccccttac ttcttatgac gaatacgcgg
     8821 gttcaccacg ccatacagca gatcgaccag caggttgacg aggataatca tcgtcgccac
     8881 cagcaatacg ccgccctgca ccaccggata atcgcgccgt tgcagtgcgt caatcaacca
     8941 gcgccccaga ccgggccacg agaagatggt ttcggtcaga atcgcccccg ccagcaatgt
     9001 tcccacctgg aggccgataa cggtcactac cggcagcatc gcgttacgca gcgcatggac
     9061 gataatcacc cgcatgcggg tcaacccttt ggcgcgcgcg gtgcggatgt aatcttcacc
     9121 cagcacttcc agcatcgagg agcgtgtcat acgcacaatg accgccagcg gaatagtacc
     9181 cagcacaatg gcgggcaaga tcatatgggc gacggcatcg ataaagttac cgtcttcacc
     9241 ccagatggcg gtgtcgatca gcataaaacc ggttaacgga ttggaatcat cgaggaacac
     9301 catatcgctc acgcgaccgg agacgggtgt caggttccag tgcaccgaaa ccagcatgat
     9361 cagcatcatg ccccaccaga agataggcat tgaataacct gtcagcgcca ggccaaccgc
     9421 tgtgtgatcg aaaatggaac cgcgtttaac cgcggccagc acaccaaccg gaatgccgac
     9481 cgcggtcgca aaaatcatcg cgcagacgcc aagttccagc gtggcctgaa agcgcggcac
     9541 gaactcttcc cataccggaa ttcggctttt cattgaaatg cctagatcgc catgcataac
     9601 gccccaaatg taatggagat actgctgcca catcggttta tctaagccga gttcagccag
     9661 caactgcgcg tgacgctctg gggagatccc acgttcgccc gccatgatca tcacaggatc
     9721 gcccgggatc atgtggacaa aggcaaatgt gagaagggta ataccgataa acgtggggat
     9781 gacgagtccc aaacgtcgga gaataaactg caacataacc cggattctct gtagtgaccc
     9841 gcagcctgga actgcgtgtc tgtattgctc acaaatcaat tcccgcccag gtcgggcagg
     9901 gaatgaagta ttgctgccgg atggcgctgc acgcagaggc acagcaccac cgggtatggc
     9961 ttttaattat tcgatagaga cgttttcgaa gtgatgtttg cctaatggat caaccacata
    10021 gcctttaact tctttgcgta ccggttcaaa cacggtggag tgagcgatga tcagtgccgg
    10081 agcctgatcg tgcatcacca cctgcgcttg cttgtacagt tcaacgcgtt tgttatggtc
    10141 gtcggtagca cgcgccggtt gaatcagatc ttcaaacggt ttgtagcacc attttgagta
    10201 gttggagcct tgttcagagg cggcgcagct gaacagagtg gcgaagaagt tatccggatc
    10261 cccgttatcg ccagtccagc ccatcattac cgtctggtgc tcgccatctt tcgcacgctt
    10321 gaggtactca ccccattcgt aggtgacaat tttggcctgc acgccgactt tcgcccagtc
    10381 tgcctgaatc atctccgcca tgcggcgagc gttcgggtta tacggacgtt gtaccggcat
    10441 cgcccacagg tcgatggaga aacctttttc cagacccgct tctttcagca aggctttcgc
    10501 tttttcagga tcgtaggtgt agtcctgaac gtcgtcgtta tagccccaca tggttggcgg
    10561 gatcaggttt ttcgctgata cacccgcgcc ctgataaacc gctttgatga tcgcgtcttt
    10621 gttcaccgcg taggtcagag cctggcgaac tttcacgtca tcgagtggtt ttttctgcac
    10681 gttatacgag agataaccga cgttcagccc cggcatttcc atcaggttga tagatttatc
    10741 ctgcttcatg cgggcgatat ctgccgggtt cgggtacggc atcacctggc attcattttt
    10801 ctgcaattta gcgtaacgca cggaagcgtc aggggtaata gagaaaacca gcgtatcgat
    10861 ctgcggtttg gtgccccagt agccatcaaa cgctttgtag cggatacggg aatctttttg
    10921 atactgctgt aactggaacg gaccggtgcc gattgggttg aggtccagtt tttccggtgt
    10981 accggctttc atcatcgcat cagcatattc ttttgacaga atagaggcga agtccattgc
    11041 caggtcagcg aggaacggcg cttccgggcg agtcagcaca aactgaacgg tgttgtcgtc
    11101 cacctttttc acttcgctga tcagctccgg caagcccatg ccttcgaagt attcgtagct
    11161 gccgccagaa actttatggt acgggttttg cgcgtttttc tgacgatcga acgagaacac
    11221 cacgtcatcg gcattcattt cgcgcgtcgg tttgaattct ttattgtcgt gccacttcac
    11281 acctttacgc agatggaagg tataggtttt accgtcttcg ctgacttccc acttttcagc
    11341 gaggcccgga atcacttcgg tggtgccgat tttaaattca actaggcggt tataaagcgg
    11401 tacggaagag gcgtcatagg tggtaccgga ggtaaacagc tgcgggttaa acccttccgg
    11461 agatccttct gagcaataaa ccagagtttt agcctgaaca cttgctgcga cggtcatagc
    11521 caccaggctg agaccaagct tcagcatccc tgactttttc aaggaaatac gcattattct
    11581 gctccaattg tgatgtttgt tgttttaacc ctttgcagtg ggttgtcgct gcctgacctt
    11641 ttttgttttt tgcccggtcg ggtcaacgtt atgaggtggg gatgccgtac taattaacat
    11701 cagtgtaaca gtgcggatcc tccataaaat gcccctcgtg acctacaatc tgtcaacaga
    11761 atgtgaaaac gtcaatacag ccaaccggga tttacaccaa cggtgagaat ccacaaacaa
    11821 agattaaaaa aacttcaaac agctatttgc agcagagaaa tttgtgctac tccaacatga
    11881 ccagaacaat cagcttaata tttagcaaca tttggtgatt aagttttatg catttagtga
    11941 aaaaaatctg aggaaaaggt ggatatctga gcgattaata ccatgaacgc taaaacgcac
    12001 agcggaaaat gccagcgcca gccataagta acgcaaaaaa agatttaagc aaatataaaa
    12061 aaagacaatg gtttctgtca caaatccgtt aatggatagt gagatatggg gcgcaaattt
    12121 ggcagggaaa tgtgtggcgt gtaggtcgaa taaggcgttc acgccgcatc cgacaataag
    12181 tgcggtgccg ttgcctgatg cgacgctgac gcgtcttatc aggcctacgt cgagtcgaac
    12241 ctccctgccc ccggaggttc tcttcctcat gggctgcaga tgcaaaaaag cctgctcgtt
    12301 gagcaggctt ttcgaatttg gtcggtgata gaggattcga acctccgacc ccttcgtccc
    12361 gaacgaagtg cgctaccagg ctgcgccaat caccgaatgc ggggcgcatc ttactgcgca
    12421 gatacgccct cgtcaatccc ttaatagcaa aatgcctttt gatcggcgag aaagtcagca
    12481 ggcctcttag ttagccgctg cctcttttgt ctgcgggatg tgacaccagt tgttgttttc
    12541 gttaattcca ccatccggtg aagtatagcc aagacaaccc atgatggtgt cgtacagctc
    12601 tacgtgacgg cgtggcactt tcatgtcggc tgcttttttc agctgcgcaa acgcctgcgc
    12661 attgaccgga ttttccagat atttatctga catccagacc atcatcggta cgcggaactg
    12721 ctccggcggt gccagttcac gcggcgtgcc gtgcaggtgc tcgcgttcat taattgactc
    12781 gccgtggtca gctgcgtaga acacaatcgc tttcttatcg cgaacctgat caatcacact
    12841 ggagataaag tgatccacat aggtcaccga gttgtcatag gagttgatca tctgcgcttt
    12901 ggtacatccg ctatcaacac caatacattc cggcttccac tgcgcaaagc tgcgcgggta
    12961 acgctgggtg tagttaaagt gcgaaccttt ggtatgcaga atgattagat gcttaccatc
    13021 cgggttgcgc cccagcgatt gctgcatttc gtctaccagc aacatatcat ctaccggctt
    13081 gccacggtta cgtggctccg caccaatctg ttcacggtaa gcaatattgt ccgccatcgt
    13141 attgctgtag aaccacattt cgctctgcat agcgtagagg tcagaactga atcctaactg
    13201 cttcagtacc gcgaaaatgt tctgttcttt taatgtgcgc tgcggattat cttccgcgcc
    13261 cccctgacgt acaaacatgc aacgcagaga gagtttggtt gcggtatcac aggagtaacc
    13321 acggaacgcc gccagatttt tctcctgggc cagcttcggc gtggtattac gctcatagcc
    13381 gaaaatgccc atatggtccc aacgcgtggt ttcaccgatg ataaacacga cataagtatc
    13441 attaacattt tgcggtgcct gataagtgaa tttcttcgcc gggttaagca atgaattatt
    13501 atcggaagat tcatcgaccc gcgcccaggc gtacagcccc aacgcagaaa gccagtttga
    13561 tggcagataa gagttcgcta cgacaccgcc ataactcggc aaatcaacgc cggtcgccct
    13621 ctccactttc ttctgctgga tatccagcaa acgaatcggt gcccaaacca taattcccgc
    13681 cagtacgacg accgccaggc tgcgaatacg ctgccctggg gttcgcagtt gtcggagcaa
    13741 ggtgtagcga cagcggttat tccagataag gataagaggc aatgcgctaa cggcgattaa
    13801 ccagagaata aagttcagac caacaacttc ttttgacagg tcgatatcgg tggtcatgac
    13861 ggaagcgatg atgccataac caatgaccac attaaggaag gtcatgtaat agctggcacc
    13921 tgcggaaaag agcacaacca gcgatgccag aatacgccag ctgcggcggc caaacagcga
    13981 aagaagacgt agtaaaaaga aggtcaccag tacggtggcg gccagttcaa caacagcaga
    14041 aatgcctttc cagacggtaa aatcgtgcgc atagctgccg aagcggcggt aaaaaaccgc
    14101 gccattcata aaaaggccaa tatagattgc aagcaaaaag ctcagcttct gctgtgtaat
    14161 cgatttgatg tatctcatgc aaacaaaccc tggacaaaca ggtgataaca ccctgcttag
    14221 cggcttttca ggcccagcag gggaaaagtg cggaggtagt cagataagta aaatctacac
    14281 gcgcgaataa gtagaccaca gcaaagggaa aaagtgtggg gaaagagtgt gcatgaagca
    14341 caaatttaca aaagtgcaag tccggaatag accattttaa cggattaacg actattcaga
    14401 gataggttgg gccaggatgc tggcccagta acttactcat aggtgatgga aaaatttaca
    14461 agggcattcg ctgtgccacc agtggcctga gtgttgacga ccacataact cgcctggtag
    14521 tgaagcgacg tggtttcgcc atcaatcgcc cattcaatcg gcgttccgct ggagcctttg
    14581 ggaacttgct gcgttccttt atcgtcctct atcttcaacg caacacccgt tgcgccacca
    14641 gcctcaaggg caaacatgga atcatcatcg ctgtccgact gactgtccat ctcaacggtc
    14701 gctttggata tcccatcgca ctctttgagt tcaatggtga aatctttctt gctcaacacc
    14761 gtaccgactg ccgcaggttt ttgcagcgtc attgttccca tatcgacttc gagatcgctt
    14821 ttttccatct cgcaagcttt agtggtgata gtgatggaga aatgaatatc ccctgccggg
    14881 ccaagatccg tcgccgcatt caccccgcag gcaaccagac tcaaactggc gatgccgcta
    14941 tgcagcaatc gtttgaactt catggcatca ctccatttca agatatatgg tcgttacgcc
    15001 ttcaaacgtc ccgcctttag gcgctttacc cgtcgtgcta atcggtaaag ccagcagtcg
    15061 aatcgcggca ttgcggtgag ttttagaatc caggctgctc aaatttaata acgtggtgct
    15121 gcttgaatca ttcggtacca aaattttgcc gctggaatct ttcaccagta cgccaatatc
    15181 ggggttgccc atatccaccg ctcggggatc gtcagcattg gttgccccct caaggcgcaa
    15241 cgcaaccttg ataccatcag agatattgtt gcaatcgaag ctgaggttaa tttcttgctc
    15301 tgtcgcacct tcaggcattt gcccttgcct gtttttaaac gccgaggagg ggaattcacc
    15361 aaatggcaca tccagcaccg tgccgggttt aatttcgcaa tcctgggtta cggtgacacg
    15421 cccctgaata gatacggaag atatgggtat tgttccgtaa tctttatttg ttttcgaggc
    15481 atacagttcg gcaattaacg tcagagggat gacggaatcg ccgataaagg gtttagtaat
    15541 agcaaaggaa agtagacctg cgccgcccat atagggcgta gtaatgactt tgttgcatcc
    15601 ggttgtttgc agcgcctgct ggtttttgaa aggaacaaac ccatacacac tggtactgat
    15661 gcgaatgttc gtcgcaacct ccagttcctc cgtcaaagta taatatttca tactgtcaac
    15721 cgtacggcca gaagggggaa gcatactcac cccctgcact agagtatctt tttcattctt
    15781 ataagaatca ggacattcac aaattagcga tacgttttgt ccattagacc agttaaggac
    15841 atccggtaaa accgttcctg cgacgttttt tgccgcatca ggtataccaa catccacatc
    15901 gacattatag tgataagtcg tgccatccgc gggggtacat ggcccccacg catctccggc
    15961 aaatgccttg ccagaaaatc cgaatacgca tcctaataag gataatgcta ttgccgcttt
    16021 catttcgctc tccttgctta ctggcaattg gccgaaacag gaataattcc gccctgtttt
    16081 aattccaggg ataatttata aggcgccacg cattgctgcg acgcgtcatt accccattta
    16141 atcaataccc gtccttcctc tggcattccg ctaatataga gctgtccttc ttcaccaacg
    16201 atactgctca cctcgttttg tttatcggtg attagcgttg ccgttgctcc aaagggcacc
    16261 gggctgcccg cggaacgaag cagcgtcatt aatacgcggt aaccaacgcg ggtatcaaaa
    16321 cgcgctctga caatcgctcc acgcgttggc accactttct ggaacgcatt ttcaacgtca
    16381 acatcgtcac ccaacgaatc gctacgcagt gagatttcgt tttcacgata cggcatggca
    16441 taaggcacga tcgcgttacc catccagtca acctgaacgc cgccgttatt caccacgcgg
    16501 gcattgcgcg caccgggtac ggagatgagc gtcatggttt cgcctagcgg ttgggaaagc
    16561 gtcacgcctt cgctatgaac tatcacgccg ccgcgaacgc tgtagttgag ctgccggtaa
    16621 tctttcccgt agctatagcc tacgttgaca ttaccggacc caccctgata gccaacgtta
    16681 gcgttaccgc tgttacccac accgttgctg gcgtagcctt cctggacact gtagctcagg
    16741 ttgcgatcct caagcagcgt tccgctgacc ccaacctgtt gattggtacg accatcctgg
    16801 tcggtcgtga cgcgatagtt actccaggcc cggcctagtg gaacggaaat attgaaagac
    16861 cacaagcgat cgctttcatc ccattcaggg cttttattcc agctatatgc aatactgtaa
    16921 ctgaccttgc caatacgtcc gttgtaaccc gccgataccg tgttctgttt acctgcgtcg
    16981 ttccagtaat cctgctgcgt taaatttaaa taaacagagc cataggcccc taattgctgc
    17041 gtcaggttac cctgtatttc gctgcgcttg tgataacggt tatagtcgct gtcagcgtca
    17101 ctgcgcacat cggtggcttc ctggaaggta tagaaaccgc tggtagagta ccgatagccc
    17161 gcaaggcgga aatcggtgcc gctttcgaag ctcttggaat ataagaaacg ataagattgt
    17221 ccctggctat cgcgatcgtt attcagttcg cttttagcct gcgtcacatc aatggaaatc
    17281 gccccgatat aaccaaagtt tttccctatc cctaatgtaa atgcattgta attattagag
    17341 attaatacgc cgccgtaggc cgtcatcccc cacggtaaac cgtagatcag atcaagctgc
    17401 ccgaaacgcg gcgaggcgct gtcataattc ccggcctgat attcacctgc ggcaaagcta
    17461 tatttgagat ggccttcacg ctggaaaatt ggcaccgatg agaatggcta aataaagcgt
    17521 tgttcactgc catccgcttc tttaataatg accgtgagat caccgctgtt tgcagtgggg
    17581 taaagatcgt ttatttcaaa ggcaccaggc tgcacatagg tgcgataaat aacgtagccg
    17641 ttttgttcaa tactgacttc ggcgttgctt ttggcgatcc ctcgaacgat gggtgcaaag
    17701 cctcgctggc tgtcgggcag catctcttca tcagaggcta acatcgcgcc acgcatctga
    17761 acgctgtcaa aaatgtcccc cgcggtcgcc gtatccccca ttgttaattg cgctttgaaa
    17821 gggatgatgg ccctactcaa agacgtaccg atattatccc actgcgcttt gccaccggaa
    17881 tggctccagg tactgtaatt ccgtaaccgc caagcgccaa ggttcagccc gctgcgtaag
    17941 ttgaggtagt agctgtcgct ctttagcgta tctttaccat catcatgatg aacagtgcca
    18001 ttatcatcga cataactgct ggaagacccc gtcgagtcat attcactgtt gctcccgcta
    18061 aaactgtaat ccagcagcaa cgcagggata ccttcgtccc ataaagattc cggcaccgtg
    18121 ccgcgcgcca cctggtgcat cgccgcctga gggaaactta ataccagctt ttgttcactg
    18181 aagtcgaaat ggcttgaggc caggggaatg atttcgtcaa acggtacgca ggcttcggca
    18241 gacaccagat tgagggccgg aaagctttca acgcgcacgc ccatctctgc cagttgcgtc
    18301 cggctaaggc aaggcacaag ggattccccc atcgctttgc gctgtgcttc tgtggcctct
    18361 ttaaattcca gtgtgcgggt ttccattctc tgttcattga cgtaaacaga gacacgatag
    18421 atgccgggta actgctgttc ctggcctttt tcgaacatcg ttaaatcgac atgctggccc
    18481 acaccctgaa cgtcctccag gaatttgggg ttaaactttc cggcatgtgc agaaaacgtg
    18541 ctggcaacag caaccgccac cacagagata gaaaaggaag aaaaagtacg tgaaacggtc
    18601 ttccgtgaca ttgagctatt tctcctgaaa agatattaat caatcaacat ttttagtgat
    18661 catttccgct gacgatccaa agtcattgat ggtgttatat ttaattttag aaacgctacc
    18721 taccccggcg ggtaa
//
LOCUS       DAZFDC010000092        18367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFDC010000092 DAZFDC010000000
VERSION     DAZFDC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.092"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            37..1176
                     /gene="arnB"
                     /locus_tag="ACHJ48_003300"
     CDS             37..1176
                     /gene="arnB"
                     /locus_tag="ACHJ48_003300"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO4708789.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADINAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPSLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1180..2148
                     /gene="arnC"
                     /locus_tag="ACHJ48_003301"
     CDS             1180..2148
                     /gene="arnC"
                     /locus_tag="ACHJ48_003301"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO4708790.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHILVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2148..4130
                     /gene="arnA"
                     /locus_tag="ACHJ48_003302"
     CDS             2148..4130
                     /gene="arnA"
                     /locus_tag="ACHJ48_003302"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO4708791.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHHCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4127..5017
                     /gene="arnD"
                     /locus_tag="ACHJ48_003303"
     CDS             4127..5017
                     /gene="arnD"
                     /locus_tag="ACHJ48_003303"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO4708792.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5017..6669
                     /gene="arnT"
                     /locus_tag="ACHJ48_003304"
     CDS             5017..6669
                     /gene="arnT"
                     /locus_tag="ACHJ48_003304"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO4708793.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6666..7001
                     /gene="arnE"
                     /locus_tag="ACHJ48_003305"
     CDS             6666..7001
                     /gene="arnE"
                     /locus_tag="ACHJ48_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO4708794.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAISKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7001..7387
                     /gene="arnF"
                     /locus_tag="ACHJ48_003306"
     CDS             7001..7387
                     /gene="arnF"
                     /locus_tag="ACHJ48_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO4708795.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7381..7647)
                     /gene="pmrD"
                     /locus_tag="ACHJ48_003307"
     CDS             complement(7381..7647)
                     /gene="pmrD"
                     /locus_tag="ACHJ48_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO4708796.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKVAGKTQ"
     gene            complement(7757..9112)
                     /gene="menE"
                     /locus_tag="ACHJ48_003308"
     CDS             complement(7757..9112)
                     /gene="menE"
                     /locus_tag="ACHJ48_003308"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO4708797.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9109..10071)
                     /gene="menC"
                     /locus_tag="ACHJ48_003309"
     CDS             complement(9109..10071)
                     /gene="menC"
                     /locus_tag="ACHJ48_003309"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO4708798.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10071..10928)
                     /gene="menB"
                     /locus_tag="ACHJ48_003310"
     CDS             complement(10071..10928)
                     /gene="menB"
                     /locus_tag="ACHJ48_003310"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO4708799.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10943..11701)
                     /gene="menH"
                     /locus_tag="ACHJ48_003311"
     CDS             complement(10943..11701)
                     /gene="menH"
                     /locus_tag="ACHJ48_003311"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO4708800.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILSFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRSEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11698..13368)
                     /gene="menD"
                     /locus_tag="ACHJ48_003312"
     CDS             complement(11698..13368)
                     /gene="menD"
                     /locus_tag="ACHJ48_003312"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO4708801.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIACRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13457..14752)
                     /gene="menF"
                     /locus_tag="ACHJ48_003313"
     CDS             complement(13457..14752)
                     /gene="menF"
                     /locus_tag="ACHJ48_003313"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO4708802.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14831..15136)
                     /gene="elaB"
                     /locus_tag="ACHJ48_003314"
     CDS             complement(14831..15136)
                     /gene="elaB"
                     /locus_tag="ACHJ48_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO4708803.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15191..15652)
                     /locus_tag="ACHJ48_003315"
     CDS             complement(15191..15652)
                     /locus_tag="ACHJ48_003315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4708804.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15717..16634
                     /gene="rbn"
                     /locus_tag="ACHJ48_003316"
     CDS             15717..16634
                     /gene="rbn"
                     /locus_tag="ACHJ48_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO4708805.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPSPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16822..18045
                     /locus_tag="ACHJ48_003317"
     CDS             16822..18045
                     /locus_tag="ACHJ48_003317"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO4708806.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIKLIKNACLKLYNLAHTATK
                     HPLKSHDSDNLLFKKLFSPSKLMAIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFW
                     DKNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNIKSDPSWP
                     KEVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSF
                     NSFNCDPTRETPNNKNITDHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQMES
                     NSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLENRNCYLDKYGDANINHYYRNLEI
                     KNSHPKNRASSKRVS"
BASE COUNT         4451 a         4881 c         4556 g         4479 t
ORIGIN      
        1 taagtggaca ggtattcaat ggcggaagga aaagcaatgt cagaattttt gcctttttcg
       61 agaccagcaa tgggcgtgga ggaactcgct gcagttaaag aggttctcga atccggttgg
      121 atcacaaccg gtccgaagaa tcacgcgctt gaacaagcct tttgccagtt gacgggaaat
      181 cagcatgcca tcgcggtcag ttcagccacc gccggaatgc atatcacgct aatggcgttg
      241 gaaattggca agggcgatga agtgattacg ccttccctga cctgggtttc aaccctcaat
      301 atgatttcct tgcagggtgc aacgccggta atggtggatg tcgatcgcga tacgctgatg
      361 gtcacgcctg aagcgatcga atcagccatt acgccacgca ctaaagccat cattccggtg
      421 cattatgccg gtgcgccagc agatattaac gccattcgcg ccattggcga acgttacggc
      481 atcgcagtta tcgaagatgc tgcccatgcc gtaggtacgt attacaaagg gcgacatatt
      541 ggcgcaaaag gtaccgctat tttttcattt catgccatta aaaatattac ctgtgcggaa
      601 ggaggcctga ttgttactga taatgaaaat attgcccgcc agctacggat gctgaaattt
      661 cacggcctgg gtgtcgatgc ctatgacaga caaacctggg gccgtgcacc gcaggctgaa
      721 gtcttaacgc cgggctataa gtacaatctg accgatatta acgccgcgat tgccctgaca
      781 cagttagcca aattagagca cctcaacacc cgtcggcgcg aaattgccca gcaatatcag
      841 caagcactgg cagctctccc ctttcagcca ttaagccttc ccgcctggcc gcacgttcac
      901 gcctggcatc tgtttattat tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg
      961 ttgatggaag cgttaaaaga aagaggtatt ggtaccgggt tacatttccg cgccgctcac
     1021 acacaaaaat attatcgcga gcgttttccc tcgctgtcgt taccgaatac cgaatggaat
     1081 agcgaacgca tctgttcttt gccgctgttc ccggatatga ctaccgccga tgccgaccgt
     1141 gtcatcacag cccttcagca actcgcagga caataagcca tgtttgaaat ccaccctgtt
     1201 aagaaagtct cggtggttat tcccgtttat aacgagcagg aaagcttacc ggaattaatc
     1261 aggcgcacca ccacagcctg tgaatcgttg gggaaagagt atgagatcct gctgattgat
     1321 gacggcagta gcgataattc cgcgcatata ctggtcgaag cctcacaagc ggagaacagc
     1381 catattgtat ctattttgct taaccgcaat tacgggcaac attcagcgat tatggcggga
     1441 ttcagtcacg ttactggcga cttaattatt acccttgatg ccgatctcca gaatccgcca
     1501 gaagaaatcc cccgcctggt ggcaaaagcc gatgaaggtt acgacgtggt agggactgta
     1561 cgccagaacc gccaggacag ctggtttcgt aaaacagctt cgaagatgat taaccggctt
     1621 attcagcgca ccaccggcaa agcgatgggt gactacggtt gtatgctgcg cgcctatcgc
     1681 cgtcatattg tcgatgcgat gttgcactgc catgaacgca gcacctttat cccgattctg
     1741 gcgaatatct tcgcccgccg tgccattgaa attccagtgc atcatgccga gcgtgagtat
     1801 ggtgaatcca aatacagctt tatgcgcctg attaatttga tgtacgacct ggtgacctgc
     1861 cttaccacaa cgccgctacg tatgctgagt ctgctcggca gcattattgc gattggaggt
     1921 tttagcattg cggtgctgct ggtgatttta cgcctgacct tcggaccaca atgggcggca
     1981 gaaggcgtct ttatgctatt tgccgtgctg tttactttta ttggcgctca gtttatcggc
     2041 atgggattac tcggtgaata tatcggcagg atctacaccg atgtccgcgc ccgcccccgc
     2101 tattttgttc agcaagttat ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg
     2161 tttttgccta ccacgatatg ggatgcctcg gtattgaagc cctgctggct gccggttacg
     2221 aaattagcgc catttttacc cataccgata atcccggtga aaaagccttt tatggttcgg
     2281 tggctcgtct ggcggcggaa agaggcattc cggtttatgc gccggataac gttaatcatc
     2341 cgctgtgggt ggaacgcatt gcccaactct caccagaggt gattttctct ttttattatc
     2401 gccatcttat ttgcgacgag attttgcagc tcgctcccgc aggtgcattt aatctgcacg
     2461 gttcgctgtt accaaaatat cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg
     2521 aaacagaaac tggcgttaca ttgcaccgaa tggtaaaacg tgccgatgcc ggggccattg
     2581 tggcgcaact gcgcgttgcc attgcgccag acgatatcgc tattacgctg catcataaat
     2641 tgtgccatgc cgcgcgccag ctactggaac agacattacc cgccattaaa cacggtaata
     2701 ttctggaaat cgcccagcgc gaaaacgaag ccacctgttt tggtcgcaga acgccggatg
     2761 acagtttcct cgaatggcat aagccggcat ccgtactgca caacatggta cgtgccgttg
     2821 ccgatccgtg gccgggtgcc ttcagctatg ttggcaatca gaaattcacc gtctggtcgt
     2881 cgcgtgttca tcctcatgcc agcaaagcac agccggggag cgtgatttct gttgcgccac
     2941 tgctgattgc ctgtggcgat ggcgcgctgg aaatcgtcac cggacaagcg ggcgacggca
     3001 ttactatgca gggctcgcaa ttagcgcaga cgctgggcct ggtgcaaggt tcacgcttga
     3061 atagccagcc tgcctgcacc gcccgacgcc gtacccgggt actcatcctc ggggtgaatg
     3121 gctttattgg caaccatctg acagaacgcc tgctgcgcga agatcattat gaagtttacg
     3181 gtctggatat tggcagcgat gcgataagcc gttttctgaa tcatccgcat tttcactttg
     3241 tcgaaggcga tatcagtatt cattccgaat ggattgagta tcacgtcaaa aaatgtgatg
     3301 tcgtcttgcc gctggtggcg atagccacgc cgattgaata tacccgcaac ccgctgcgcg
     3361 tatttgaact cgattttgaa gagaatctgc gcattatccg ctactgcgtg aagtaccgta
     3421 agcgaatcat cttcccgtca acttcagaag tttatgggat gtgtagcgat aaatacttcg
     3481 atgaggacca ttctaattta atcgtcggcc cggtgaataa accacgctgg atttattcgg
     3541 tatcaaaaca attacttgat cgggtgatct gggcctatgg cgaaaaagag ggtttacagt
     3601 tcaccctctt ccgcccgttt aactggatgg gaccacgact ggataacctt aatgcggcac
     3661 gaatcggcag ctcccgcgcg attacgcaac tcattctcaa tctggtagaa ggttcaccga
     3721 ttaagctgat tgatggcgga aaacaaaaac gctgctttac tgatattcgc gatggtatcg
     3781 aggcgttata ccgcattatc gaaaatgcgg gaaatcgctg cgacggcgaa attatcaaca
     3841 ttggcaatcc tgagaacgaa gcgagcattg aggaactggg cgagatgctg ctggcgagct
     3901 tcgaaaaaca tccgctgcgc catcatttcc caccgtttgc gggctttcgc gttgtcgaaa
     3961 gtagcagcta ctacggcaaa ggatatcagg acgtagagca tcgtaaaccg agcatccgca
     4021 atgcccacca ctgcctggac tgggagccga aaattgatat gcaggaaacc atcgacgaaa
     4081 cgctggattt cttcctgcgc accgttgatc ttacggataa accatcatga ccaaagtagg
     4141 cttacgcatt gatgtcgata cctttcgtgg cacccgtgaa ggcgtgccgc gtctgctgga
     4201 aatcttgagt aagcataata ttcaggccag catttttttc agcgtcggcc cggacaatat
     4261 gggccgccat ctctggcgac tggtgaagcc acagtttttg tggaagatgc tgcgctcaaa
     4321 cgcggcatcg ctttatggct gggatatttt actggcaggt acggcctggc caggtaaaga
     4381 gattggtcat gccaatgccg atatcattcg tgaagcggct aaatatcacg aagtcggcct
     4441 gcacgcctgg gatcaccatg cctggcaagc ccgtagcggt aactgggatc ggcaaacaat
     4501 gatcgacgat attgcacgcg gccttcgcac tctggaagag attatcggtc aaccggtaac
     4561 ctgttctgcc gctgcgggct ggcgtgccga ccagcaggtg atcgaagcaa aagaagcgtt
     4621 ccatttgcgc tacaacagcg attgtcgtgg ggccatgccg ttccgtccac tgctcgaatc
     4681 aggaaaccct ggcactgcgc aaattccggt gaccttaccc acctgggatg aagtgattgg
     4741 tcgggatgtg aaagcagaag attttaacgg ttggttactc aaccgcatcc tgcgagataa
     4801 aggcacgccg gtttatacca ttcatgcaga agttgaaggc tgcgcttatc agcataattt
     4861 tgtggatctc ctcaaacgcg cagctcagga aggcgtgaca ttttgccctt taagcgaact
     4921 gttatcagag acgttgccgc tcggacaagt tgttcgcgga aatattgccg gacgtgaagg
     4981 ctggctgggt tgccaacaaa ttgcgggtag tcgctgatga aatcggtacg ttaccttatc
     5041 ggcctcttcg cgtttattgc ctgctattac ctgttaccga tcagcacgcg tctgctatgg
     5101 caacccgatg aaacgcgtta tgcggaaatc agtcgagaaa tgctggcatc cggcgactgg
     5161 attgtgcccc atctgttagg gctacgttat tttgaaaaac ccattgccgg atactggatt
     5221 aacagcattg ggcaatggct atttggcgcg aataactttg gtgtgcgggc aggcgttatc
     5281 tttgcgaccc tgttaactgc cgcgctggtg acctggttta ctctgcgctt atggcgcgat
     5341 aaacgtctgg ctctactcgc cacagtaatt tatctctcat tgtttattgt ctatgccatc
     5401 ggcacttatg ccgtgctcga tccgtttatt gccttctggc tggtggcggg aatgtgcagc
     5461 ttctggctgg caatgcaggc acagacgtgg aaaggcaaaa gcgcaggatt tttactgctg
     5521 ggaatcacct gcggcatggg ggtgatgacc aaaggttttc tcgcccttgc cgtgccggta
     5581 ttaagcgtgc tgccatgggt agcgacgcaa aaacgctgga aagatctctt tatttacggc
     5641 tggctggcgg tcatcagttg cgtactgacg gttctcccct ggggactgac gatagcgcag
     5701 cgggagcctg acttctggca ctattttttc tgggttgagc atattcaacg ctttgcactg
     5761 gatgatgccc aacatagagc tccgttctgg tactacgtgc cggtcatcat tgccggtagt
     5821 ctgccgtggc tgggattact ccccggtgca ctatacacag gctggaaaaa ccgcaagcat
     5881 tccgcaaccg tctatttgtt gagctggacg ataatgccgc tgctgttttt ctccgttgct
     5941 aagggtaaat tgcccaccta tattctttcc tgctttgcac ctttggcaat gctgatggcg
     6001 cattacgctt tgctggcagc aaaaaataat cctctggcgc tgcggattaa tggctggatt
     6061 aacatcgctt ttggcgtcac tggcattatt gccacatttg tggtctcccc ctggggacca
     6121 atgaacacgc cggtgtggca aaccttcgag agctataaag tcttttgtgc ctggtcgatt
     6181 ttttcgctat gggcattttt cggctggtac accttaacaa acgtcgaaaa gacctggtct
     6241 tttgccgcgc tttgcccgct ggggctggcg ttgctggtag gattttcaat tcctgacaga
     6301 gtcatggaag gaaaacatcc gcaatttttt gtcgagatga cacaagaatc acttcagcca
     6361 agccgctata ttcttactga tagcgtcggt gttgccgcag gtctggcatg gagtctgcaa
     6421 cgcgatgaca tcatcatgta tcgccagaca ggtgagttga aatacggcct taattatccg
     6481 gatgcgaaag ggagatttgt cagcggtgat gagttcgcaa actggcttaa tcaacatcgt
     6541 caggagggga ttattacact cgtgctttcg gttgaccgcg atgaagatat caacagtctc
     6601 gccattccgc ccgcagatgc catcgatcgt caggagcgtc tggtgctgat tcagtatcgt
     6661 cccaaatgat ctggctaaca ttagtctttg ccagcttgct tagcgttgcc gggcagttgt
     6721 gtcagaaaca ggcaacctgc tttgtggcga taagcaaacg gcgcaaacat atcgtgctgt
     6781 ggctgggact ggcgctggct tgtcttggtc ttgccatggt gctctggctg ctggtcttgc
     6841 agaacgtacc ggtaggtatt gcctacccga tgttaagtct gaattttgtc tgggtgacgc
     6901 tggctgcagt aaaactgtgg cacgaaccgg tatcgccgcg tcactggtgt ggggtggcgt
     6961 tcattattgg tggcattgtg atcctcggga gtacggtgta atgggcctga tatggggatt
     7021 attcagcgtc attattgcct cagttgcgca attaagcctg ggttttgcgg cgagtcatct
     7081 gccgccgatg acgcacttat gggattttat cgcggcgctg ttagcttttg ggctggatgc
     7141 ccggatactg ttactcgggc tgctgggtta tctgctttcc gttttttgtt ggtataaaac
     7201 actacataaa cttgcgctaa gcaaagccta tgccttgctc agtatgagtt atgtgctggt
     7261 atggattgct tcaatggtac ttcccggctg ggaagggact ttttcgctga aagcactact
     7321 gggagtagcc tgtattatga gcgggttgat gctgattttc ctgcccacga caaaacaacg
     7381 ttactgagtt ttccctgcca ctttacactg ccgttcccac tcatcggggc tataacagct
     7441 tgcagagagg actttcaccg tatgcagctt ttcacgatta atacaataaa gcgcattctg
     7501 caaaggcgag agtaaatccc ccactttcac ggcgaagtca ctcttcactt cggcgatcat
     7561 ttttatcgcg ccgccagcat cgcagagcat aagtacatgt ctgttatctt gtttattgca
     7621 gcacgatttt ttgaccagcc attccattgt attatcctgt ttgctaagag ttttcatatc
     7681 aatttacaga ttaacagata aactctttat caacggcaat ggaatggcag aactgagaag
     7741 tgaaacggag ggttaattat tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa
     7801 ttttaatacc gccgtttttc agctccggcg gcaaagtcag ccaacgtacc ggttgctgaa
     7861 aacgggccag cttatctttc acccattcac taagatcaac gctctcgtgg tcatactcca
     7921 tcaccgccac cggtcgatga ccaaactcct tgtcggcaac ggggacgata aacacctgca
     7981 aaaccgcagg atgtgcagca attacgcgct cgacttcttc cgtctgaata ccctctccgc
     8041 cactgaagaa tagattgtct aaacgtccga caatggtcag cttgccatta tgcatctcac
     8101 cgcgatcgcg cgtagcgtac cagccttcgt cattaaccag tgaaaccagt tgcccgttac
     8161 gccagtaacc ttctgccata ctggcagccc gcagccacac ttcattatta acgattttca
     8221 cttcccgacc cggcagcggc gaaccaacgt ctgccaggcc gtcggcttct ttcgcacaca
     8281 ccgtggaggc aaactcggtc agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg
     8341 cctgttccgt caactcgacc gggatagccg cgccgccaag taacaccgct ttcagggaaa
     8401 cggaactgcg gttaaccagc aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac
     8461 agcctgccag catttgctcc aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt
     8521 ataaccagcg ccacataatt ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc
     8581 agtcatcgtg atcgccaaac ggaatcagcg ataacacccc ttcggcactg gcaagatggg
     8641 cctgataagt atggacagcg gctttcggca acccggtaga acccgaggtc aacgtcattg
     8701 agcacagacg cgtcggctgc cacgtagcga catgtgcgcc ttcaacccgc tgaatgtgca
     8761 gcgacgttaa tgccggaaac gtgttttccc catccggcac cagcgcaaat tgcagcgtca
     8821 gattgggcag caattcttca agcaacggtt gcggcagctg agggttcacg ggcaacaccc
     8881 gcgccccgca ttgcagtaac gccagccaag cgagcagcgt ttgcggcgta ttccacgccc
     8941 gcaacatcac gccgctgtcc tcaaccacac cctgcaccgc aaatccggag gctaattcat
     9001 cgacgcgagc acaaagctcg cgccagttga gttgctcgtc attaagacgt aaggcgatgg
     9061 cttctccccg cacttgccgc cagtgacgcc acggccagtc agagaagatc ataacaaccg
     9121 ctccagtgca tcaacttcca cgacaggcag cgtgctaccc ggccagcgac gtacctgctg
     9181 cgcctgcatt agatccagcg tgtccagccc tggaatggtg tccggcgtta accaggcagc
     9241 aatccgcgcc agttgcgtta agcctaagct cgattcaatg gaagaactga tcactgccgt
     9301 cagccccagc gcgtgcgccg cctgtacctg ctcgcgaact ttctccagac tgcccgtgag
     9361 cgtgggtttg ataactaccg cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc
     9421 gcgcaagctt tcatcccagg caatggcaat gccggtttca cgggcaaacg ctcgcgaatc
     9481 atcgcgggtt ttgcacggct cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac
     9541 gtatttggca aactgctgac ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa
     9601 atgcagatcc ggaattgcct ccagcaacag attcaccacc atgccgtcgc gcaccgcttc
     9661 gtacaatccc actttgacct tcgccacttt ctcgcctggc atatctgcaa gtttgaggat
     9721 cagatcgtcc ggatcgccat tacacagtgg tgccgtacgg taattggccg cctgcgggag
     9781 cgtatctgcc agctccgcca atgcacaact tacgccaaag gcaacggaag gcatctgcgg
     9841 taattcgcaa tcgcctgcca gccagttatt tacccaggca agcagcacac tttgcgcctc
     9901 ttcccaggtt tcctgactga agcccggcag tggggagatc tccccccacc cttcgcgctc
     9961 gccctcacgc aggcaaacgt acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac
    10021 cacccccgcg tccatgggga tctgccagcg gtatacctgc gcgctacgca ttacggattc
    10081 cgtttgaatt tgctgaagtc aggctgacgt ttctggttga aggcgttgcg accttcctga
    10141 ccttcttccg tcatgtagaa cagcatggtg gcgttgcccg ccagctcctg cagacctgcc
    10201 tgaccgtcac agtcggcgtt cagtgcagct ttcaggcagc gcagcgccat cggactgttt
    10261 tgcagcattt cacggcacca gcgaacggtt tctttttcca gatccgccag cggtaccacg
    10321 gtgttgacaa ggcccatatc cagcgcctgt tttgcgtcgt actgacggca caggaaccag
    10381 atttcacgcg cttttttctg cccgacgata cgagccatgt aggaagcgcc ccagccgccg
    10441 tcgaaggaac cgactttcgg gccagtctga ccgaagatgg cattatctgc cgcgatagtc
    10501 aggtcgcaca tcatgtgcag aacgtgacca ccgccgatgg aatagccagc caccatcgcg
    10561 acaaccggtt tcggacaggt acggatctga cgctggaagt ccagcacgtt caggtgatgt
    10621 acgccggaat catctttata gccgccgtaa tcaccacgca ctttctggtc accaccggag
    10681 cagaacgctt tatcgcctgc gccagtcaga atgatcacgc cgacgttatc gtcataacgc
    10741 gcatctgcca gtgcctggat catctctttt accgtcagag gacggaaggc attgcgcacc
    10801 tgcggacgat taatggtgat ttttgcgata ccgtcggtgg atttttcata acgaatgtcc
    10861 tcaaagcctt cggagcagtc gtgccattca accggtgcgt aaagcattgc ttcatcagga
    10921 taaatcatat tgtgtccttt agtcagaaac gcaagatctg cgccagactt gcgattacgc
    10981 cagcgggatt ttcccgatgc gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag
    11041 ccagttccgc cgccagggcg cggaatttgc tgtcacgttc accacataaa taataaaacg
    11101 caaatgtgcg ggcgctaagg ttagcacgta aatcaggctg gacggcgaga gaagtcgcct
    11161 ccagcatggc ggcaagggtt gcgccattat tgttgctgcg cagcaccacc agctcccggc
    11221 gttgatcgtc attgagcgag gcaaaaacag gctgttgata ccagtcggca aataccgcgg
    11281 ttaatggttc ggaacgaaaa cgctgcgccc attggcgatc ggaacgctga cgttccgcgc
    11341 gttgttcagc attttgcagc cccggatgac cgccttcgac aacaacccca caaagccccg
    11401 ccagcccctg gcaagcggcc atcatcgcca cccgtccgcc aagcgagtac cccaccagcc
    11461 agaagctaag gatgttgtaa ctaaccaggg ttttacgcag taagtcggtg acatcatcaa
    11521 atccatcgac gctaatcgcc gccgaaccac cgtgacctgg gagatcaaca tacaaccgtg
    11581 agtagtcggc aaacgcctcg ccgacttcct gccattcgag gcaatcgccg gaaaaaccgt
    11641 gcaaaaacac cagccagggt aaacctggtt tgccgtgttt tgcctgcgcg tgcaggatca
    11701 taaatggctt acctgcgcca gcagctgctg gagcgtttgc gcgccgtcgg tgtcgttaac
    11761 caccatttca attaccgtgg tggtcggagt acgccaggcg tcggcaagta ccgtttcaag
    11821 ttcctgccag ttttgcggac gatgatattt cagctcaaac atcgcggcag cgtgctcaaa
    11881 atggacgttt tgcggcatca gatagaaacg ctcgcgctcg cttttcggcg ttggcagcag
    11941 cgagaaaatt tgcccgccgt tgttgttcac cacaattaat accagcggcg cggaaacctg
    12001 gcgcaataac gccagcgcat tcaggtcgta aagtgcggag agatcgccca caatcgccag
    12061 cgtcggtttg ccgcttgccc gctgaacgcc ggcggcagtc gagagcagcc cgtcgatacc
    12121 gctggcccca cggttgctgt acaccgggta acctgccgga agttgcgaaa gcgcatcaat
    12181 cagacgtacc accaggctgt taccgacaaa caattgcccc tgttcaggca gatagtcgct
    12241 gatgcgatgc gccagttgcg cttcgccaaa cgcatcgcga caggcaataa ccgcctgcat
    12301 tgcctgttcc gccaggcgcg ggatttcaac gcaccagggc tggcgttttt ctgccggatg
    12361 cagttccagc cagtcggcaa tattggcaat taagcggcgt ccacgatggt gtgccggatc
    12421 aagccgccct tcgatgtcat ccacaatcca gtactcttcg ggttcacagc ttgcctgcca
    12481 ttgcaggagc cgtttgcccg tcaggctgct tcccagttgc accacaattt gcgcctgttg
    12541 cagttcgctg gtcgctttgg cattgcctaa ccagagatcg gcacacggca gcggctgtcc
    12601 ggtttgcgac agcacgtcgc caatcagcgg ccagccaaga gtttgcgccc acagggcaac
    12661 ttttttgccc tcttccgcac tcatgcgccc ggcaaccacc acgccgcgct tttgccgcca
    12721 gaagaaccag tcgcgctgtt tttcgctctc aagacgaggc gcttcacgca gccacggctt
    12781 atcatcctgc caccagtcac ccagacgctg ttgccagcta agcccagtgt cgtccatctc
    12841 gccatacagc ggttcagcaa acgggcagtt gatatggacc ccaccagcat gaagcgtacc
    12901 gagagcatgg tcgatggtag aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg
    12961 cagtgaaata ctgtgcgtgg ggtgagaggc gaacattccc ggctgacgaa tcgcctgatt
    13021 cgcgccgcag tcaattagct ccggcgggcg atcggcggtt aacaggatca gtttttctcc
    13081 ggttaaccca gcttcaatca gtgccggata gagatttgct accgccgtgc cggaggtcac
    13141 aatcaccgcc accggctgct tgctgacttt cgccagcccc agcgccagat gccccagtcc
    13201 acgctcatcg aaatgggtgt ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa
    13261 cggtgtagaa cgcgagccag gggcgatgca gatgtgtctt acgccgtgac gcgttaatgc
    13321 ttccagaatg accgccgccc agcgtcggtt aaatgcgctt actgacatga gtttgtccgg
    13381 tatcaatatt gcggctaagt ataaggagct acaaaaatag attattgata tgaatcggta
    13441 atgatgcgac tcattactat tccatttgta ataaagtacg cagccctgcc gctttgttgt
    13501 cgatttcctg ccactcttgc tcggggtcgg aaccacggac aatgcccgcg ccagcatata
    13561 gccgcacgac attgccgctg atttttgctg agcgcaggga aacgcagaat tcgctttgtt
    13621 gtaatgagag atagcccgca gaaccggcgt accattctcg ggtgaacggt tcgtgatggg
    13681 cgataaactg tcgcgccaga tcgcgcggta agccagcaac tgctgccgtt ggctgcaact
    13741 gatgtaaaca gatcacatca tccgctttgt tgagtgaagt ccagatacag cggcgaagat
    13801 gctgcacttt acgcagacgc agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg
    13861 cctgtaatcg ttggcagata tcttccacca ccagcatgtt ctcgcgctgg tttttatcat
    13921 ccgccatcag ccactctcct aactgctgcg cctgcttatc atcaggatga tttgctactg
    13981 ttcccgccag cgcttcagta cgcagcgctt tgtcacgccg ccgccataac cgttccggtg
    14041 aagagccaag aaaagcattt tcgccatcaa aggccatgta aaaatggtag caattgagat
    14101 tcagtcgacg actggcagcc atcatcgccg ccgcattgac cggacttgcg aaatgcaggt
    14161 cagttgcccg agcaagcacc actttgtcga gctcaccttc ggcgatggtt ttcgttgcca
    14221 gttcgattaa ttgcgtccag cccgttttgt ccggccagtg ttgttctcgc gtagtggtta
    14281 aatgtaaccc aggcaagggc ttgatactca ccagtgtggc gataaattct cttgccttaa
    14341 tcgcatcgtg ctgaagggag ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc
    14401 cacagcgtcg ccattccagg cgtggtaaaa gtaaattgcc ctgcgacggg tcaaacgcat
    14461 tcagccccca aatgcgtaag tcggcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct
    14521 ggtccaacga cgtaaaacgg gtaatcgcgc ccaggacggc agcttcttca tcaccattac
    14581 gttgttgcca gtagaattgc gggtacgttt gctgactggc cagccagctc aaggcatcaa
    14641 aagcgtcttt gagagggaaa ggaatatcga taacccgaat gccgggtgtc gccggaatct
    14701 cttgcgacaa atggcgcagt agattttcca gcgccgtagt aagtgattgc acgcggacct
    14761 ctccctgtta aaagcctcac attatacggg gtactacaaa aaaaatgcag taccccggtg
    14821 tagggaggtt ttaacggcgt gccagcaaca gtcctagtac cagcccaacg gccgcaccca
    14881 caccaattcc ctgccagggt ttttcgtgga cgtagtcatc agcacgataa acagcctgct
    14941 tcgcccgata gtaataactg tctgaagcct ggctaacccg ttttttcaca tcatccagcg
    15001 ctttttctgc acgcgctttc agctcaacat atttctgatc ggcgggatcg ccagaggagc
    15061 ggagcacctc ttccagtgtt tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac
    15121 caaactgatt agacattctc gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa
    15181 gggttattga ttacgcctga attacctcgc gtgccatccc aatgtgtggg ataccatctt
    15241 cttcatagat ctccgtcaca gggataaaac cgaaactctg gtagaagttt tgcagatgcg
    15301 cctgcgcccc gaggtagaca ggcttatcag gccagtgatg cgtgcaagtg tcgagggttt
    15361 tactcatcag ttgctgcccc actttttcgc cacgcaacgc ttcactgaca atcacccgac
    15421 caataacgac cggctcaaga tcatcatcac ttttcagaat cctcgcatat gccaccagtt
    15481 catcattttt ccaaccgaga atatggcgat tatcccccgt caaatcatca ccatcgatat
    15541 cctgataagg gcaattttgc tcgacaacaa acaccgcgca acgcagttgt aataaggcat
    15601 aaagctggga aaccgataat tcagagtgat gcagatcttg ccattcaatc ataatgctct
    15661 ccttttagat gtatgcctta tactagccgg aatatttttt gaaacgtgat gaactcatgg
    15721 aattaatttt tttaggtact tcggccggtg tgccaacccg cacgcgcaat gtcacggcaa
    15781 tattgctaaa cctgcaacat ccgacccaga gcggactttg gttgtttgac tgcggtgaag
    15841 gcacccagca tcagctactg cataccgcct ttaaccctgg aaaactggac aagattttta
    15901 tcagtcacct tcatggcgat catctttttg gtttacccgg cttgctgtgc agtcgttcta
    15961 tgtcaggcat tatccaaccc ttaacgattt atggtccgca aggtatccgt gaatttgtgg
    16021 agaccgcgct gcggattagc ggctcatgga ccgattatcc gctggaaatt gtcgaaattg
    16081 gcgctggcga aattctcgat gatggcctgc gcaaagtgac cgcttatcca atggaacacc
    16141 cactggaatg ctatggctat cgtattgaag aacatgataa accgggtgca ttaaatgccc
    16201 aggcattaaa agctgctggc gtaccgccta gcccactgtt tcaggaatta aaagcgggca
    16261 aaacaatcat gctggaagat ggaaggcaga ttaacggcgc agattactta gctgctccag
    16321 tgccaggtaa agcgctcgct attttcggcg ataccggccc ctgcgatgcc gcacttgacc
    16381 tggctaaagg tgtcgatgtc atggtgcacg aagcgacgct ggatataacc atggaagcca
    16441 aagccaatag tcgcggtcat agctctacac gccaggctgc gacactggcc cgtgaggctg
    16501 gagtcggtaa gctaatcatt acccacgtca gctcgcgcta tgatgacaaa ggttgtcagc
    16561 acctgttacg tgaatgcagg tcaattttcc cggcgactga actagcgaat gatttcaccg
    16621 tgtttaacgt ttaactctgc ttattattaa aaacagggcg aaacttgccc tgttatcgca
    16681 acccgcgcta catatatcca ctcacacggg catattgagc gatttttgat atttattcat
    16741 ctccttacgc actatattca gagcaataag ttgcctcttt ttatcttcat tcagcacaac
    16801 ctttgcaact aaaaggagca tatgatggtt acagttgtca gcaattattg tcaattatct
    16861 caaacccaac tcagtcagac atttgcagaa aaatttactg tgaccgagga attactgcag
    16921 tctttaaaaa aaacagcgtt atccggagat gaagaaagca ttgagttact gcataatatt
    16981 gcgttaggtt atgatgaatt tgggaaaaaa gctgaagata ttctttacca tattgttaga
    17041 aacccaacaa atgataccct atcgattatc aagcttataa aaaatgcctg tttaaaatta
    17101 tataatttgg cacataccgc aaccaaacac cccctcaaat cacatgattc agataatctc
    17161 ctgttcaaaa aactattctc cccttcgaaa ttaatggcaa ttatcggtga ggacattcct
    17221 ctcatatcag aaaaacagtc actttcaaag gtacttttaa atgataagaa taatgaactg
    17281 agcgatggga caaacttctg ggataaaaat cgtcaattaa ccacagatga aatagcttgc
    17341 tatcttaaaa agatcgccgc caatgcaaaa aatactcaag tcaattatcc tactgatttc
    17401 tacctcccca attccaacag cacttacctg gaagtcgctc tcaatgataa tattaagagc
    17461 gatccatcat ggccgaaaga agtccagtta ttccccataa atactggcgg acactggata
    17521 ttagtttcgc tacagaaaat agttaatgaa aaaaacaaca cacaacaaat aaaatgcatc
    17581 atattcaatt cattacgtgc actaggccat gaaaaagaaa attcacttaa gcgtatcatt
    17641 aacagtttca atagtttcaa ttgtgacccc acgagagaaa cgccgaataa taagaatata
    17701 acagatcatt taactgaacc agagataata tttttacatg ccgatcttca gcaatactta
    17761 agccaaagtt gcggtgcatt tgtgtgcatg gcagcccagg aagtgattga acaaatggaa
    17821 agcaattctg acagcgcccc ctatacgtta ttaaaaaact atgctgacag atttaaaaaa
    17881 tattcagcag aagagcagta cgaaattgat tttcaacatc gtctggaaaa cagaaattgt
    17941 tatttagata aatatggcga tgcaaatatc aatcattatt atagaaactt agaaataaaa
    18001 aactcacacc ccaaaaatag agcatcaagc aaaagagtaa gttaacatca ttgttaatta
    18061 attcgaccag tcagcaaata tgctgactgg tcgaacatag tttgctaaaa agtcatcact
    18121 gactgatgtc agtcacacca cccgccagtt caatcttcgg gtatttccag cgtatctcgg
    18181 cacgcgattg ctggtctgtt gatgtagtgg attgttgttg ctgacttttc ttaccctcag
    18241 atttctgccc cctgtcaact caatctgcca ataaataaca taattatatt tttccttata
    18301 tgaattcctt tttaaaatag cgaagccact gtaaaagata ctttttgtat agatctaaca
    18361 accgtaa
//
LOCUS       DAZFDC010000093        18202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFDC010000093 DAZFDC010000000
VERSION     DAZFDC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.093"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..754)
                     /gene="aroD"
                     /locus_tag="ACHJ48_003318"
     CDS             complement(<1..754)
                     /gene="aroD"
                     /locus_tag="ACHJ48_003318"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO4708807.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLALLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQ"
     gene            complement(785..1651)
                     /gene="ydiB"
                     /locus_tag="ACHJ48_003319"
     CDS             complement(785..1651)
                     /gene="ydiB"
                     /locus_tag="ACHJ48_003319"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO4708808.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDKF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENKSLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1663..2928)
                     /locus_tag="ACHJ48_003320"
     CDS             complement(1663..2928)
                     /locus_tag="ACHJ48_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4708809.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3155..4369)
                     /locus_tag="ACHJ48_003321"
     CDS             complement(3155..4369)
                     /locus_tag="ACHJ48_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4708810.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQSNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFSFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4469..4825)
                     /locus_tag="ACHJ48_003322"
     CDS             complement(4469..4825)
                     /locus_tag="ACHJ48_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO4708811.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGRCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4961..5068)
                     /gene="rprA"
                     /locus_tag="ACHJ48_003323"
     ncRNA           complement(4961..5068)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHJ48_003323"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5254..6366)
                     /gene="ydiK"
                     /locus_tag="ACHJ48_003324"
     CDS             complement(5254..6366)
                     /gene="ydiK"
                     /locus_tag="ACHJ48_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO4708812.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEIPPPTDQPEEILEELGEIQKPNK"
     gene            6755..9811
                     /locus_tag="ACHJ48_003325"
     CDS             6755..9811
                     /locus_tag="ACHJ48_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO4708813.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQMCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9808..10218
                     /gene="menI"
                     /locus_tag="ACHJ48_003326"
     CDS             9808..10218
                     /gene="menI"
                     /locus_tag="ACHJ48_003326"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO4708814.1"
                     /translation="MIWKRKIPLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEISDEKGRLCCSSRLTTAIL"
     gene            10318..10506
                     /locus_tag="ACHJ48_003327"
     CDS             10318..10506
                     /locus_tag="ACHJ48_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO4708815.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11055..11423
                     /gene="sufA"
                     /locus_tag="ACHJ48_003328"
     CDS             11055..11423
                     /gene="sufA"
                     /locus_tag="ACHJ48_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO4708816.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11432..12919
                     /gene="sufB"
                     /locus_tag="ACHJ48_003329"
     CDS             11432..12919
                     /gene="sufB"
                     /locus_tag="ACHJ48_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO4708817.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVVSDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12929..13675
                     /gene="sufC"
                     /locus_tag="ACHJ48_003330"
     CDS             12929..13675
                     /gene="sufC"
                     /locus_tag="ACHJ48_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO4708818.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13650..14921
                     /gene="sufD"
                     /locus_tag="ACHJ48_003331"
     CDS             13650..14921
                     /gene="sufD"
                     /locus_tag="ACHJ48_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO4708819.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLELAEGAEATVIEHFVSLNDARHFTGARFSINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENCTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLTRIGQRLPGGAR"
     gene            14918..16138
                     /gene="sufS"
                     /locus_tag="ACHJ48_003332"
     CDS             14918..16138
                     /gene="sufS"
                     /locus_tag="ACHJ48_003332"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO4708820.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKKALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSAQGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16151..16567
                     /gene="sufE"
                     /locus_tag="ACHJ48_003333"
     CDS             16151..16567
                     /gene="sufE"
                     /locus_tag="ACHJ48_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO4708821.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDVVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16716..17720
                     /gene="ldtE"
                     /locus_tag="ACHJ48_003334"
     CDS             16716..17720
                     /gene="ldtE"
                     /locus_tag="ACHJ48_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO4708822.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17783..18019)
                     /gene="lpp"
                     /locus_tag="ACHJ48_003335"
     CDS             complement(17783..18019)
                     /gene="lpp"
                     /locus_tag="ACHJ48_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO4708823.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4778 a         4411 c         4672 g         4341 t
ORIGIN      
        1 cctgatgtaa aatagttaat actgtgcgca aatcatttac cgagatttgc cctggcgcag
       61 aggctttttt taccgcacca aaagttgccg ccgagccaaa tacttcacca gccagacgag
      121 aaattacgcc agtttttgcc attgacatcg tgatgattgg acgatcggca tactgctcct
      181 gcatctccag ggtcgcggca agcaacgcca gcacatcgct ggtactttgc ggcatcagcg
      241 caatcttagg aatatcggcg tcgaaggact gcattttgcg cagacgggca atgatttctt
      301 cggcttccgg cgttttatgg aagtcatggt tggacatgac aactttcaca tcatgcgcgt
      361 gggcgtaggc gacggtttct ttaacctgat catcgccggt aaataactcc agatcgatca
      421 tatcaaccag gccgctgtcg atggctgcac gattgagagc aatataagcc tcggtggaaa
      481 tcgcctgctc gccgccttct ttggcactgc ggaaggtaaa cagcagcggt ttttctggca
      541 tggtttcacg gagaattttt gccgccgcca tgacagactc cacattggag aggtcggcaa
      601 agtggtccac acgccattcc agaatatcaa agtccgcttc acgataggcg agagcttcgg
      661 atttcactcg ggcgatatct ttcgccatca gcgagacgat gattttaggt gcgcccgtac
      721 caatgacgag atcttttaca gttacggttt tcatttttta ccctttctgc acgcggtcag
      781 cctgtcaggc accgaacccc atgacctgtt taacatattc cagagggaaa tctttgccag
      841 tccataacgt gaactgttca gccccttgcc acaacaacat gccgtatcca tcaatcgttt
      901 tgcaaccagc ttgttgcgcc tgctgcaata acttcgtcat atgcgggtta tacacgcatt
      961 cagtgaccag cagtcccgga tgtaacagac tgatatcatt aaccaatgat ttattctcaa
     1021 ggggtttcat acccactttt gtgccattgg ttaaaatgtc ggcggaagcc agggcttcag
     1081 caaaggcttg ctgatcggcg agatcggtaa ccgtgacgac acaatcggtg ttttcgttaa
     1141 cccgctgcgc gaaggcgagg gctttatcga agaacttatc ccgacggtta aagagtttaa
     1201 tttcttttaa accttcaatt gccccctgcg cgccaatcgc cgttgaggca cctccggccc
     1261 ctaacagcac cattgttttg cctttgatat caaaaccgct ctctttaatg gcgcgaatat
     1321 ggcccgtgcc gtcggtgtta tagccgcgca ggtagccatc atcattaacg atggtgttga
     1381 tggcacccac cagtttggct gctggcgtta attcatcaac atattcacac gccagttgtt
     1441 tgttcggcat tgatacacca gttccgcgca tttttagagc ttttaatcct tcaattgctc
     1501 cgggaaagct atcgttatcc acttcgaagg ccatataggt aaatggcaat cccgcttttt
     1561 ctaaggcttt attctgcatt tcaggcgata aactgtggcg gataggataa gccatcaacc
     1621 caatcaattc gtattttgcg gtaacatcca taattaactc ctttaacctc tatgcttaat
     1681 tgtgtttaac cgggtgctaa atttgcgctc gccaaaccgc acatcattct caggaataat
     1741 aaaaacgcgg taatagcgga taaaaacaat aattgcggta atcagggcca gcagcgcgaa
     1801 agtaaaatcg agaacaatga tatattgcag gccgatgttc gacagataac cggtaatcag
     1861 cggaataaca aagttagcca gtccacccat catcatataa atactggtga ctttggcttt
     1921 gctttttggg aaaaactctg acatgaccga aacaccgagc tgtaaaatgc cgccagctgc
     1981 tgagaaaccg ataacaaagg cgccggcatt acacaccagt ggagaagggt acaggtagat
     2041 aatggctgct gttattgttg ccagtgcaga gttaaataca ttagcccaga tgggccggac
     2101 catttttttc agtagtgcgg caaaaataaa gacacagacc agcgagccca tactgtaata
     2161 agagatggtt tttaatgcct cagcttctga cataccagca aaagccatcg catatttggg
     2221 catccacacc acaatcacat aaaaggtcga gaatgcggct acaccgaaca gtaccgatga
     2281 aacaccttcc agccagacta acggtttgct gttcatttgc ggtaattcat tagctacgct
     2341 ggcgtccacc aactggctgg ggaatttgct tttcaacagc atcagcgtga tcagtacaaa
     2401 cagaataccc ggaataatca gcccatagcc gtaccagata ttattgagca acatatagct
     2461 caccagcatt gggtagaaca tttgcccaaa tgacaccatc gctttaacca gtatgaccgc
     2521 cgaaccagag gctttcggaa agcattccat gagcgcgggg tagccacccg tatccagcgc
     2581 tgagttagcg atacctacgc acactgccag accgtaggcg agagttaaat tcgggcaagc
     2641 gggaatacca aagaagaata gcagatacat tattactgcc attaatatca ccgcccgacg
     2701 accaaactta tcggagatca caccgaagaa taaaatactg atcaatcgcc ccaaaccgat
     2761 accggaaatt aagtaggcaa tgcccgcgtt gtcagtggaa aacttttccg ccagagatga
     2821 catattttgg gcaagcgtaa taacactaat gccgtgcagg aagtagctga agtaaataca
     2881 aagaacagcc aggataaatg gcgtgctgaa agccttattt tgagacattt tcacagctcc
     2941 ttgctgaaag ataatttcgt taccgtacgt attgttctcc gacggtttac ataattacta
     3001 tactgaagat gattttccct gggtacatgg cataaagttt atgtttccag gaatattgca
     3061 tgtcatagtt tgtttttgat cgcgattttc cttagttagc gcagaaagag ttatttatcc
     3121 gtaagatctt gcatgacctg aaaagaatat tgcattaccc acccggagcg acattttcct
     3181 ttagcgagtg atgccgcgtt tttttgcggc tgcgtaagcc gataaacagt gccagtaaaa
     3241 aaccgatggc agcgatggcg gtatcgaacc acataatatc ggcaatactt ctttgggaca
     3301 gatgagccgt aatcaacgga atagtaaagg tcgcaatact gcccgcactg taatagatcc
     3361 ctgtagcttt acctttagcg taagggaaac gttcagccat taacgtcagg ccaatttgca
     3421 caacacctcc agcagaagta aaaccaatga caaaagaaaa tattatcacc acataaaatg
     3481 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
     3541 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
     3601 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
     3661 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
     3721 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
     3781 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
     3841 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
     3901 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
     3961 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
     4021 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
     4081 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
     4141 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
     4201 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagactgcc
     4261 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
     4321 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
     4381 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccgttcat tattgttgta
     4441 acaacgacaa ttcctttttg tggagaaatt aacaaagccg ctccagatcg tgtgcatata
     4501 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     4561 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     4621 ttttctctat gatcgcatga agatgttttt ttctttgctg acgcatagcc aacagttgct
     4681 cgactacaca atcaggaatg gcacacctgc cattttccca ttgacgccag ctttcactgt
     4741 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     4801 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     4861 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     4921 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     4981 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     5041 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     5101 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactaccagc
     5161 tgaatggcgg taagcaggaa aatatgggga agagcaagca aatatcaatt agcggtggtt
     5221 aacaatcagg cggcagagcc gcctgatgag aaattactta ttcggtttct ggatttcgcc
     5281 aagttcttcg agaatttctt ccggttgatc cgtcggcggt gggatctctt ccacccacgc
     5341 ggcaaacaga cgccaggaaa cggctaacag aaccggacca ataaacagac cgatcatccc
     5401 gaaagcaatc aaaccaccaa taacgccaga gaggatcagg atcagcggta aatcggcacc
     5461 catgcgaatt aacattgggc ggatgacgtt atccagcgtg ccaaccacac cgctccacac
     5521 taacaatacc gttccccagg tggtatcgcc agtccagtag agccagataa tcgccggaat
     5581 cagtaccggc aacgggccaa gctggacaag gcaggaaagg atcattaaca ccgttagcaa
     5641 agttgcataa ggtacgccgg atacggcgag gccgataccg ccaagcactg cctgtactaa
     5701 cgccgtcacc accacaccca gcgccaccgc gcggatagcc tgtgccgcca gcagtacggc
     5761 ggcatctccg cgaacgcctg ccagacgggt cgcaaaatgg cgaatgcctt gtgccacctg
     5821 ttcaccgcgc cagtacagca gggcactgaa gagaagcatc agcgcacaat gcaccataaa
     5881 gcgcccgata tgcgccgctt gcccaacgaa ccaggtagtg gtggtgccaa tataagggcg
     5941 gactttcgcc atgatcgccg tcccccccat atccagcaag ttgtgccagc ctgcatacag
     6001 cttcgcgcca atcaccggaa tggtattaag ccacgccaga tcgggtaacg tcatgtcacc
     6061 gctggaaatg gctttaatta gcgggccgct gccgtcgacg atactgttaa ccagcaaagc
     6121 aatagggatg ataaacacca tcactaataa cagcgtcatc accagaacgg cgagagagcg
     6181 gcggccaaac atgatctttt gcaaacgtaa caataccggc caggtggcga taaccaccgt
     6241 accggcccat gcaaagccga gaataaaggg ttgaacaatc cacagacatg ccacaatcat
     6301 gatggctaaa aacagcaccg aaagcagaat ttgtgcgaca tccctgggct gacgaacatt
     6361 taccataact acttttcacc ttttttgtgc gtcaaatcgt cgacgcgacg tgaacacgcg
     6421 aaactcacct gcataatgat gagcaatttc agcggttttt aacaggccga ttctgccctt
     6481 aattctgatg ggcgtgttag aaaaaaatgt ggtacaacaa ctcaggcaac acgcaaacga
     6541 tttactcgtc gtatttcaag ttgcatatgc tgcggctacg ttcgttcacc ccagttactt
     6601 actgttgtaa gctcctggag ttcactcgct tgccgccttc ctgcaactcg aaatccatag
     6661 agtaaataaa catcaccttt ctgtgcgcta ttgcagccgc ctgaaagaac agtgtatcga
     6721 cctacatcac agaccgcagg aaagggtcaa tataatgatt ccacagattt cccaggcacc
     6781 cggcgtcgtt caactggtgc ttaatttttt gcaagagctg gagcaacaag gttttaccgg
     6841 cgatacggcg acaagttatg ctgatcgtct gacaatgtcg accgacaaca gtatttacca
     6901 acttctcccc gatgcagtgg tatttccgcg ttcaaccgca gatgtggcgc tgatcgcccg
     6961 tcttgccgcg caggaacgct attcatcgct gatctttacc ccccgcggcg gcggcaccgg
     7021 cactaacggt caggcgctca accagggtat tattgttgat atgtcccgcc atatgaaccg
     7081 catcatcgaa attaaccctg aagagggctg ggtgcgcgtt gaggccgggg tgataaaaga
     7141 ccaactcaat cagtacctga aaccgttcgg ctactttttt gcaccagaac tttcgaccag
     7201 caaccgggca acgctcggcg ggatgatcaa taccgatgca tccggtcagg gatcgctggt
     7261 ctatggcaaa acgtcagatc acgtacttgg cctacgtgcg gtattgttgg ggggcgatat
     7321 tctcgatacg caacctttac ccgtcgagct ggcggaaacg ctgggtaaat ccaataccac
     7381 aatcgggcga atttataaca cggtttatca acgttgccgt cagcaacgcc agttaatcat
     7441 cgacaacttc cccaaactta accgctttct taccggttac gatctgcgtc atgtctttaa
     7501 cgatgagatg accgagttcg atctgacgcg cattctgacg ggttcagaag ggacgctggc
     7561 ctttattacc gaagcgcggc tggatattac gcgcttgcct aaagtgcgcc gtctggtgaa
     7621 tgtcaaatat gactcttttg actctgcgct gcgtaacgcg ccgtttatgg ttgaggcgcg
     7681 ggcgctttcg gtagagacgg tggactcaaa agtgctgaat ctggcgcggg aagatattgt
     7741 ctggcattcc gtcagcgagt tgattaccga tgtgcctgac aaagagatgc tcgggctgaa
     7801 cattgtggaa tttgctggtg atgatgaggc gctgattgac gagcgggtaa atgcactctg
     7861 tgcgcggttg gatgagctga tcgccagtca tcaagcaggt gtcatcggct ggcagatgtg
     7921 ccgtgagctg gcgggggttg aacgtatcta tgcaatgcgc aaaaaagccg ttggtctgct
     7981 tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct gaggatacct gcgtaccgcc
     8041 ggaacatctg gccgattata ttgctgaatt tcgcgcgctg ctcgacagcc acggcttaag
     8101 ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcat gtccgtccgg cactggatat
     8161 gtgcgatccg caacaagaga ttttgatgaa gcaaatctct gatgacgtgg tggcgctgac
     8221 tgcgaaatac ggtggtttgt tgtggggcga gcacggcaaa ggttttcgcg ctgaatacag
     8281 cccggcgttt ttcggtgagg aactttttgc agaactgcgc aaagtgaaag cggcatttga
     8341 cccgcataac cgactcaacc cagggaagat ttgcccgcca gaaggtctcg atgcgccgat
     8401 gatgaaagtg gacgcggtga agcgcggtac attcgatcgg cagatcccca ttgcggtacg
     8461 ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt ttatgcttca actttgatgc
     8521 ccgtagtccg atgtgtccgt cgatgaagat cacccagaac cggattcatt caccgaaagg
     8581 gcgcgcaacg ctggtgcgtg aatggctgcg tttgttggcg gatcgcggcg ttgatccact
     8641 caaactggaa caagaactgc ctgaatccgg cgtcagcttg cggacgttaa ttgcccgcac
     8701 gcgcaatagc tggcatgcga ataaaggcga atatgacttc tcacacgaag tcaaagaggc
     8761 gatgtcgggc tgtctggcct gtaaagcttg ttcgacccag tgccccatca aaattgatgt
     8821 gccggagttt cgctctcgtt ttctgcagct ctatcacacc cgttatttac gcccgctgcg
     8881 cgaccacctc gtcgctacgg tcgagagcta cgcgccgctg atggcacgcg cgccgaaaac
     8941 ctttaacttc ttcattaacc agccgctggt gcgcaaactc tcggaaaaac atatcggcat
     9001 ggttgatttg ccgctgctgt cggtcccctc gctacaacaa caaatggtgg ggcatcgctc
     9061 ggcaaacatg acgctggaac agcttgaatc tctcaatgca gagcagaaag cgcgcacagt
     9121 gttggtggtg caggacccct ttaccagcta ttacgatgcg caagtggtgg cggattttgt
     9181 ccgtctggtc gaaaaattag gtttccagcc tgtgttactg ccattttcgc caaatggcaa
     9241 agcccagcat attaaaggtt ttcttaatcg ttttgcgaag acggcgaaaa agacggcgga
     9301 tttcctcaac cgtatggcga agctgggtat gccaatggtg ggcgtcgatc cggcgctggt
     9361 actttgttat cgcgatgaat ataaactggc gctgggcgag gagcgtggcg agtttaacgt
     9421 cttactggcg aatgaatggc tggcaagcgc acttgagtca cagccagtgg ctacagtcag
     9481 cggtgaatca tggtatttct ttggtcactg taccgaagtt accgccttgc cgggtgcgcc
     9541 agcacaatgg gccgcgatat ttgcccgttt tggcgcgaaa ctggaaaatg tcagcgtggg
     9601 ttgctgcggc atggcaggga cttacggaca tgaagcgaaa aaccataaaa attcgctcgg
     9661 gatctatgag ttatcctggc atcaggctat gcagcgactg ccgcgtaacc gctgtctggc
     9721 gaccggatat tcctgccgta gtcaggtcaa acgggttgaa ggcacggggg tacgccatcc
     9781 tgtgcaggct ttactggaga ttattaaatg atatggaaac gaaaaatccc cctggaagca
     9841 ctgaatgcca tgggtgaagg aaacatggtg gggttactgg atattcgctt tgaacatatt
     9901 ggtgatgaca cccttgaagc gacaatgcca gtagactcgc ggacaaagca gcctttcggg
     9961 ttgctacatg gaggtgcatc cgtggtactg gccgaaagta tcggttccgt tgccggttat
    10021 ttatgtaccg aaggtgagca aaaagtggtt ggtctggaaa tcaatgctaa ccacgtccgc
    10081 tcggcacgag aagggcgggt gcgcggcgta tgcaaaccgt tgcatctcgg ttcgcgtcac
    10141 caggtctggc agattgaaat ctccgatgag aaagggcgtt tgtgctgttc gtcacgattg
    10201 acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga cgcatattgc ttttggtcat
    10261 tatctgcaca gtaaacggtg gtatactgtg caggtcttgt ctaaaagcga ggatgctatg
    10321 tctactcaac ttgatcccac ccagctggcc attgaatttt tacgccgtga ccagagcaat
    10381 ctttcacctg cccaatatct taaacgtctg aaacaactag aacttgaatt tgctgacctg
    10441 cttactctct cttctgctga attaaaagaa gaaatctact ttgcctggcg tctgggcgtg
    10501 cattgatcct gacttaatgc cgcaagttcg ccaaatcgac ctggattaac ctccgtccag
    10561 gttcgatgac cttcttaata ctgcttatct acccgcctga tattgttcaa aaataatact
    10621 aatcgcagtt tgtgttaaaa cggcggctta gctttatgag attattctta tcgccccttc
    10681 aagagctaag ccactgagag tgccggagat aagcgccgga tggggtagaa acccttaagc
    10741 ctgtgtcgca cagacttaag ggttttctta tttctgaacg ctgcactttt ttacctatct
    10801 cttaaatagt cattttcaat acaacttttt ctttttcatc acagccttaa tgctccttag
    10861 ataataatca ttatctaaca atgagatacc taattcttag cgtgcctgtt aacccacaca
    10921 tcagggtcta tgcttattaa aagcaggcaa aaagcatgtt ttacggtaaa gcccctgcct
    10981 ttgctgggtt gaactgataa tcattatcac taacatgctg ttatacgctg aaagcgatga
    11041 agtgaggtaa atcgatggac atgcattcag gaacctttaa tccacaggat ttcgcctggc
    11101 aaggcttaac gctgacaccc gcagccgctg tccacatccg cgagctggtg gcaaagcagc
    11161 cgggtatggt cggcgtgcgc ttaggtgtga agcaaacggg ctgcgcgggc tttggctatg
    11221 tgctcgacag tgttagcgag ccggacaaag acgatctgct gtttgaacac gacggcgcga
    11281 agctgtttgt cccgctgcaa gcgatgccgt ttattgatgg cacggaagtc gatttcgttc
    11341 gtgaaggact taatcagata ttcaaatttc acaaccctaa agcccagaat gaatgtggct
    11401 gtggcgaaag ctttggggta taggcggtac tatgtctcgt aatactgaag caactgacga
    11461 tgtcaaaacc tggaccggcg gcccgctgaa ttataaagaa ggattcttca cccagttagc
    11521 caccgatgag ctggcaaagg ggataaacga agaggtggtg cgcgcaattt cggcgaagcg
    11581 taatgagccg gagtggatgc tggagtttcg tctaaacgcc tatcgcgcat ggctggagat
    11641 ggaagaaccg cactggttga aagcgcacta cgacaagctg aattatcagg attacagcta
    11701 ctactcggca ccatcgtgcg gtaattgtga cgacacttgc gcgtctgaac ctggcgcggt
    11761 gcagcaaact ggcgcgaacg cctttttaag taaagaggtg gaggcggcgt ttgagcagtt
    11821 gggtgttccc gtgcgggaag gcaaagaggt ggcggtggat gccattttcg actcagtttc
    11881 ggttgccact acttatcgcg aaaaactggc ggagcaggga attattttct gttcctttgg
    11941 tgaggcgatc cacgatcacc cggaactggt gcgtaaatat ctcggcaccg tggtgccggg
    12001 gaatgacaac ttctttgccg cgcttaatgc ggcggtagtc tctgacggta cgtttattta
    12061 tgtgcctaaa ggcgtgcgct gcccgatgga actttccacc tattttcgca ttaacgcgga
    12121 aaaaaccgga cagtttgagc gcaccattct ggtggccgac gaagacagct acgtcagcta
    12181 cattgaaggc tgttccgctc cggtgcgtga cagctatcag ttgcacgcgg cagtggtgga
    12241 agtcatcatc cataaaaacg ccgaggtgaa atattccacg gtacaaaact ggttccctgg
    12301 cgataacaac accggcggta ttctcaactt cgtcaccaag cgtgctttgt gcgaaggcga
    12361 aaacagcaaa atgtcatgga cgcaatcaga aaccgggtca gcgattacgt ggaaatatcc
    12421 cagctgcatt ttgcgcggcg ataactccat tggtgagttt tactcagtgg cgctgaccag
    12481 cggtcatcag caagcggata ccggcaccaa gatgatccac atcggtaaaa acaccaaatc
    12541 gaccattatc tcgaaaggga tctctgccgg acatagccag aacagttatc gcggcttagt
    12601 gaaaatcatg ccgacggcaa ccaatgcgcg caatttcact cagtgcgact caatgctgat
    12661 tggcgctaat tgtggggcgc ataccttccc gtatgtcgaa tgtcgcaata acagcgcaca
    12721 actggagcac gaggcaacga catcacgtat tggtgaagat caactgtttt actgcctgca
    12781 acgcgggatc agcgaagaag acgccatctc gatgattgtt aacggtttct gcaaagacgt
    12841 gttctcagaa ctgccgctgg aatttgccgt tgaagcacaa aaactcctcg ccatcagtct
    12901 tgaacacagc gtcggataag gaataaacat gttaagtatt aaagatttac acgtcagcgt
    12961 ggaagataaa gctatcctgc gtggattaag cctcgacgtt cgtcccggcg aagttcacgc
    13021 cattatgggg ccaaacggtt cgggcaaaag taccttatcg gcaacgcttg ccgggcgaga
    13081 agattatgaa gtgacgggcg gcacggttga gttcaaaggc aaagatttgc ttgcactgtc
    13141 gccggaagat cgtgcaggcg aaggcatctt tatggccttc cagtatccgg tggagattcc
    13201 aggtgtcagt aaccagtttt tcctgcaaac ggcacttaat gcggtgcgca gctatcgcgg
    13261 ccaggaaacg ctcgaccgct ttgattttca ggatttgatg gaagagaaaa tcgctctcct
    13321 gaagatgccg gaagatttat taacccgttc ggtaaacgtt ggtttttccg gcggcgagaa
    13381 aaagcgcaac gatattttgc aaatggcggt gctggaaccg gagttatgca ttcttgatga
    13441 atcggactcc gggctggata tcgacgcatt aaaagtggtc gccgatggcg tgaactcgct
    13501 gcgtgatggc aagcgctcat tcatcattgt tacgcactac caacgcattc tcgactacat
    13561 caagcctgat tacgttcatg tgctatatca gggacgaatt gtgaaatccg gcgatttcac
    13621 gttggtcaaa caactggagg agcagggtta tggctggctt accgaacagc agtaacgcgc
    13681 tgcaacagtg gcatcacttg tttgaagctg aagggacaaa acgctccccg caagcacagc
    13741 agcatttaca acaattgctg cgtaccggac tgccgacacg taaacatgaa aactggaaat
    13801 atacgccgct ggaagggctg accaatagtc agtttgtcag cattgcggga gagatatccc
    13861 cacagcagcg agatgcctta gcgttaacgt tagacgctgt gcggctggta tttgtcgatg
    13921 gacgttacgt gcctgcgctg agcgatgcga ctgaaggcag cgggtatgaa gtgagcatta
    13981 acgacgaccg tcaggggtta cccgatgcta ttcaggcgga agtgtttctg catttgacgg
    14041 aaagcctggc acaaagcgtg acgcatatcg ccgtgaagcg cggtcaacgg ccggcaaagc
    14101 cattgctgtt aatgcatatc acccagggcg tggcaggtga agaggtgaac actgcccatt
    14161 accgacatca tctggagctg gcggaaggtg ccgaagcaac ggtgatcgaa cattttgtca
    14221 gccttaatga tgctcgtcat tttaccgggg cacggttctc tatcaacgtc gcagcgaatg
    14281 cccacttgca gcatatcaag ctggcgtttg aaaacccgct cagtcaccac tttgcccata
    14341 acgatttgtt gctggctgac gatgccaccg catttagcca cagtttcctg ctgggtggcg
    14401 cagtgttacg acacaacacc agtacgcaac tcaatggcga aaactgcacg ctgcggatca
    14461 atagcctggc gatgccggtg aaaaacgagg tgtgtgatac ccgtacctgg ctggaacaca
    14521 ataaaggttt ttgtaacagc cgacagttgc acaaaactat cgtcagcgac aaaggccgcg
    14581 cggtatttaa cggtttgatc aacgtcgcgc agcacgccat caaaacggat ggtcagatga
    14641 ccaacaataa tctgctgatg ggcaaactgg cggaagtgga tacgaaaccg cagctggaaa
    14701 tctatgcaga tgatgtgaaa tgcagccacg gcgcgacggt ggggcgtatt gatgatgaac
    14761 agatgttcta tctgcgctcg cgcgggatca atcagcagga tgcccagcag atgatcattt
    14821 acgcctttgc tgccgaactg acggaagcac tgcgtgatga ggggcttaaa cagcaggtgc
    14881 tgacccgaat cggtcaacgg ctgccaggag gtgcaagatg actttttccg tcgacaaagt
    14941 gcgggccgac tttccggtgc tttcgcgtga ggtaaacggt ttgccgctgg cttatctcga
    15001 cagcgccgcc agtgcgcaga aaccgagcca ggtgattgac gccgaggccg agttttatcg
    15061 tcatggctac gcggcggtgc atcgcggtat tcatacctta agcgcccagg cgaccgagaa
    15121 aatggagaac gtgcgcaagc gggcatcgct gtttattaat gcccgttcgg cggaagagct
    15181 ggtgttcgtc cgcggcacga cggaagggat caatctggtc gccaatagct ggggcaacag
    15241 caacgtgcgg gcgggcgata acatcatcat aagtcagatg gagcaccacg ctaacattgt
    15301 tccctggcag atgctttgcg cacgcgttgg cgcagagctg cgtgtgatcc cgctcaatcc
    15361 cgacggtacg ttgcaactgg agacgctgcc tacgctgttt gatgagaaaa ctcgcctgct
    15421 ggcaattact catgtctcca acgtgcttgg cacagaaaat ccactggcgg aaatgatcac
    15481 gcttgcgcac cagcatggcg caaaagtgct ggtggatggc gctcaggcgg tgatgcatca
    15541 tccggtggat gttcaggcgc tggattgcga tttttacgtg ttttccgggc ataaactgta
    15601 tggccccacc gggattggca ttctttatgt caaaaaagcc ttgctacagg agatgccgcc
    15661 gtgggaaggg ggcggttcta tgatcgccac cgtcagcctg agtgaaggca ctacctggac
    15721 caaagcaccg tggcggtttg aagccggtac acccaatacc gggggcatca ttggtcttgg
    15781 cgcggcgctg gagtatgttt cggcgcaggg gcttaataac atagccgagt atgaacagaa
    15841 tctgatgcat tacgcgctat cacagctgga atctgtaccg gatctcactc tctatggccc
    15901 acaaaacagg cttggcgtta ttgcttttaa tctcggtaaa caccacgcct atgatgttgg
    15961 cagttttctc gataattacg gcattgctgt gcgtaccgga catcactgcg ctatgccatt
    16021 aatggcctat tataacgtcc ctgctatgtg tcgggcgtcg ctggccatgt ataacaccca
    16081 tgaagaagtg gatcgtctgg tgaccggact gcaacgtatt caccgtctgc tgggataaca
    16141 gggaggcact atggctttat tgccggataa agaaaagttg ctgcgtaatt ttttacgctg
    16201 cgccaactgg gaagagaaat atctctacat tattgagctg ggccagcgtc tgccagaatt
    16261 acgcgacgaa gacagaagcc cacaaaatag cattcagggc tgccagagtc aggtgtggat
    16321 tgtcatgcgt cagaatgcgc agggaattat tgaattgcag ggcgacagcg atgcggcgat
    16381 tgtgaaaggg cttattgcgg tcgtctttat tctctacgat cagatgacgc cgcaggatgt
    16441 tgtcaatttc gatgtgcgtc cgtggtttga aaaaatggca ctcacccaac atctcacccc
    16501 atctcgttca caaggtctgg aagcgatgat tcgcgcaatt cgcgccaaag ccgctgcact
    16561 tagctaaact aaagggacag ctttcatccg gttggctcac attgccggat ggtcaatgtt
    16621 tacactgacc tgcacaggag tgtttcggtt tcaggtgaac ataagcgcca tccgacgttt
    16681 cagcgtgagt ctccggcaaa tacaggaggt ttacaatgaa acgcgcgtct ttgcttacac
    16741 tcacgcttat cggcgctttt agtgccatcc aggctgcctg ggcggttgat tatccgctac
    16801 caccaaccgg aagccgactg gttgggcaaa atcaaacgta tacggtgcag gaaggggata
    16861 aaaaccttca ggccatcgcc cgacgttttg atactgcggc aatgttgatc cttgaagcca
    16921 ataacactat cgccccggtg ccaaaacccg gtacgacgat aactattcct tcgcaactgt
    16981 tattacctga tgcgccgcgt caggggatta tcgttaacct tgcagagctg cgcctttatt
    17041 attatccgcc gggagaaaat atcgtgcagg tttatccgat aggtattggc ttgcaggggc
    17101 tggaaacgcc agtgatggaa acgcgtgtgg ggcagaaaat ccctaaccca acctggacgc
    17161 ctacggcagg cattcgtcaa cgttcgctgg agcgtggcat taaattaccg ccagtcgttc
    17221 ctgccggacc aaataacccg ctaggacgtt acgcactgcg cctcgcgcat ggtaatggcg
    17281 aatacctcat tcatggcacc agtgcgccgg acagcgtcgg tttgcgcgtc agttcagggt
    17341 gtattcgcat gaatgcaccg gatattaaag ccttgttctc cagcgtgcgg acgggaacgc
    17401 cggtgaaagt gatcaacgaa ccagtgaaat attccgtgga gcctaacggg atgcgttatg
    17461 ttgaagtaca tcgaccacta tcggcagaag aacagcagaa cgttcagaca atgccataca
    17521 cactgccagc aggctttacg caatttaaag acaataaggc tgtagatcag aagttagtcg
    17581 ataaagcgtt gtatcgtcgg gcagggtatc cggttgcggt gagcagtgga gcaactccca
    17641 cagccagcaa tgcgccttca gtagagtcag cgcagaatgg tgaaccagag caagggaata
    17701 tgttacgcgc gacgcagtag tggtaaatag cagacaaaaa aatggcgcac aatgtgcgcc
    17761 atttttcact tcacaggtac tattacttgc ggtatttagt agccatgttg tccagacgct
    17821 ggttagcacg agctgcgtca tctttagcag cctgaacgtc ggaacgcatt gcgttcacgt
    17881 cgttgctcag ctggtcaact ttagcgttca gagtctgaac gtcagaagac agctgatcga
    17941 ttttagcgtt gctggagcaa cctgccagca gagtagaacc caggattacc gcgcccagta
    18001 ccagtttagt agctttcatt attaataccc tctagattga gttaatctcc atgtagcgtt
    18061 acaagtatta cacaaagttt tttatgttga gaatattttt ttgatgggaa ggcacttatt
    18121 tttgatcgtt cgctcaaaga agcatcgaac gctgggtttt agataaaaaa attgagagaa
    18181 aacaggatgc ttccatcgga tt
//
LOCUS       DAZFDC010000094        17950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFDC010000094 DAZFDC010000000
VERSION     DAZFDC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.094"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(185..325)
                     /gene="yjjY"
                     /locus_tag="ACHJ48_003336"
     CDS             complement(185..325)
                     /gene="yjjY"
                     /locus_tag="ACHJ48_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO4708824.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            421..1137
                     /gene="arcA"
                     /locus_tag="ACHJ48_003337"
     CDS             421..1137
                     /gene="arcA"
                     /locus_tag="ACHJ48_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO4708825.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1197..2549)
                     /gene="creD"
                     /locus_tag="ACHJ48_003338"
     CDS             complement(1197..2549)
                     /gene="creD"
                     /locus_tag="ACHJ48_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO4708826.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGIGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2607..4031)
                     /gene="creC"
                     /locus_tag="ACHJ48_003339"
     CDS             complement(2607..4031)
                     /gene="creC"
                     /locus_tag="ACHJ48_003339"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO4708827.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPKSGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4031..4720)
                     /gene="creB"
                     /locus_tag="ACHJ48_003340"
     CDS             complement(4031..4720)
                     /gene="creB"
                     /locus_tag="ACHJ48_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO4708828.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDIVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRSL"
     gene            complement(4733..5206)
                     /gene="creA"
                     /locus_tag="ACHJ48_003341"
     CDS             complement(4733..5206)
                     /gene="creA"
                     /locus_tag="ACHJ48_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO4708829.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5417..6286
                     /gene="robA"
                     /locus_tag="ACHJ48_003342"
     CDS             5417..6286
                     /gene="robA"
                     /locus_tag="ACHJ48_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO4708830.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6283..6930)
                     /gene="gpmB"
                     /locus_tag="ACHJ48_003343"
     CDS             complement(6283..6930)
                     /gene="gpmB"
                     /locus_tag="ACHJ48_003343"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO4708831.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6982..7494
                     /gene="yjjX"
                     /locus_tag="ACHJ48_003344"
     CDS             6982..7494
                     /gene="yjjX"
                     /locus_tag="ACHJ48_003344"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO4708832.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASIAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTTQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(7641..7967)
                     /gene="trpR"
                     /locus_tag="ACHJ48_003345"
     CDS             complement(7641..7967)
                     /gene="trpR"
                     /locus_tag="ACHJ48_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO4708833.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(8057..9994)
                     /gene="sltY"
                     /locus_tag="ACHJ48_003346"
     CDS             complement(8057..9994)
                     /gene="sltY"
                     /locus_tag="ACHJ48_003346"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO4708834.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10205..11872
                     /gene="ettA"
                     /locus_tag="ACHJ48_003347"
     CDS             10205..11872
                     /gene="ettA"
                     /locus_tag="ACHJ48_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO4708835.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11979..12146)
                     /gene="nadS"
                     /locus_tag="ACHJ48_003348"
     CDS             complement(11979..12146)
                     /gene="nadS"
                     /locus_tag="ACHJ48_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO4708836.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVARHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12180..13412)
                     /gene="nadR"
                     /locus_tag="ACHJ48_003349"
     CDS             complement(12180..13412)
                     /gene="nadR"
                     /locus_tag="ACHJ48_003349"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO4708837.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13433..14815)
                     /gene="radA"
                     /locus_tag="ACHJ48_003350"
     CDS             complement(13433..14815)
                     /gene="radA"
                     /locus_tag="ACHJ48_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO4708838.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14864..15832)
                     /gene="serB"
                     /locus_tag="ACHJ48_003351"
     CDS             complement(14864..15832)
                     /gene="serB"
                     /locus_tag="ACHJ48_003351"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO4708839.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15938..16579
                     /gene="ytjB"
                     /locus_tag="ACHJ48_003352"
                     /pseudo
     CDS             15938..16579
                     /gene="ytjB"
                     /locus_tag="ACHJ48_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
     gene            16610..17626
                     /gene="lplA"
                     /locus_tag="ACHJ48_003353"
     CDS             16610..17626
                     /gene="lplA"
                     /locus_tag="ACHJ48_003353"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO4708840.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGIPHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17658..17921
                     /locus_tag="ACHJ48_003354"
     CDS             17658..17921
                     /locus_tag="ACHJ48_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4708841.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
BASE COUNT         4280 a         4997 c         4521 g         4152 t
ORIGIN      
        1 tctcgacaat tggattcacc acgtttatta gttgtatgat gcaactagtt ggattattaa
       61 aataatgtga cgaaagctag catttagata cgatgatttc atcaaactgt taacgtgcta
      121 caattgaact tgatatatgt caacgaagcg tagttttatt gggtgtccgg cccctcttag
      181 cctgttatgt tgctgttaaa atggttagga tgacagccgt ttttgacact gtcgggtcct
      241 gagggaaagt acccacgacc aagctaatga tgttgttgac gttgatggaa agtgcatcaa
      301 gaacgcaatt acgtacttta gtcatgttac gccgatcatg ttaatttgca gcatgcatca
      361 ggcaggtcag ggacttttgt acttcctgtt tcgatttagt tggcaattta ggtagcaaac
      421 atgcagaccc cgcacattct tatcgttgaa gacgagttgg taacacgcaa cacgttgaaa
      481 agtattttcg aagcggaagg ctatgatgtt ttcgaagcga cagatggcgc ggaaatgcat
      541 cagatcctct ctgaatatga catcaacctg gtgatcatgg atatcaatct gccgggtaag
      601 aacggtcttc tgttagcgcg tgaactgcgc gagcaggcga atgttgcgtt gatgttcctg
      661 actggccgtg acaacgaagt cgataaaatt ctcggcctcg aaatcggtgc agatgactac
      721 atcaccaaac cgttcaaccc gcgtgaactg acgattcgtg cacgcaacct gctgtcccgt
      781 accatgaatc tgggtactgt cagcgaagaa cgtcgtagcg ttgaaagcta caagttcaat
      841 ggttgggaac tggacatcaa cagccgttct ttgatcggcc ctgatggcga gcagtacaag
      901 ctgccgcgca gtgagttccg cgccatgctt cacttctgtg aaaacccagg caaaattcag
      961 tcccgtgctg aactgctgaa gaaaatgacc ggccgtgagc tgaaaccgca cgaccgtact
     1021 gtagacgtga cgatccgccg tattcgtaaa catttcgaat ctacgccgga tacgccggaa
     1081 atcatcgcca ccattcacgg tgaaggttat cgcttctgcg gtgatctgga agattaatcg
     1141 gctttaccac cgtcaaaaaa aacggcgctt tttagcgccg tttttatttt tcaaccttat
     1201 ttccagatac gtaattcatc gtccgttgta acttctttac tggctttcat tttcggcagt
     1261 gaaaacgcat accagtcgat attacgagtc acaaacatca tgccggccag cgccaccacc
     1321 agcacactgg ttcccaacaa cagcgcgcta tcggcagagt tgaggagtcc ccacatcaca
     1381 ccatccagca acaacagcgc gagggtaaac aacatgctgt tgcgccaacc tttcaatacc
     1441 gcctgcaaat aaataccgtt cattaacgcc ccaatcagac tggcgattat ccatgccacg
     1501 gtaaaaccgg tatgttcaga aagcgccagc aagagcaaat aaaacatcac caatgaaagc
     1561 cccaccagca aatattgcat tgggtgtaaa cgttgcgcgg tgagcgtttc aaaaacaaag
     1621 aacgccataa aagtcagcgc aatcagcaga atggcatact tagtcgcccg gtcagttaat
     1681 tggtattgat cggcgggcgt cgtcactgcg acgctaaacg cggggaagtt ttcccagccg
     1741 gtatcattgc ctgaagcaaa acgctcaccg agattattag caaaccagct gctttgccat
     1801 tgcgcctgaa aacctgactc actaacttcc cgtttggctg gtagaaaatc acctaaaaaa
     1861 ctgggatgcg gccagttgct ggttaaggtc atttcgctat tacgccccgc aggcaccaca
     1921 gaaagatcgc cggtaccgct taaattcagt gccatattca gcttcaggtt ctgcttccgc
     1981 cagtcccctt cgggtaaagg gatatgcacg ccctgcccgc cttgctctaa cccgatgccg
     2041 ggttcaatag tcagcgccgt tccgttaact tcaggcgctt tcaccacacc aataccacgc
     2101 gcatccccga cgctaatcac aataaatggc ttgccaaagg tgatatttgg cgcgtcgagt
     2161 tcgctaagac gcgaaacatc gaaatcggct tttagcgtta aatcactgtg ccagacctga
     2221 ccggtataaa tccctatctt gcgttcttcc acgttctgat tgccatcaac catcaatgac
     2281 tcaggtaacc aaaaatggat aaaacttcgt ttccgctcca cggttttatc ctcttcctgc
     2341 accgtataaa gctcggtcac aggaatagcg ataagaggcc caacgagttt ttgcggcccg
     2401 ctggtacttt ggcgaatcgc atcttccaca tcgctacggt aatcggcacg ttcgacaatc
     2461 acctgccgaa tcagcattat cggaatcaac aacagcaata ctgcaccaaa cagggtggtc
     2521 attttccaga acagggggga tttcaacata gtcttctcct ttgcaatggc agcaggatac
     2581 gaaggctatg tgggaagaat ttgaagctat gtgaagtgac ggtgaagccg aagcgaggcc
     2641 agcacgccac cttcctgcac gttgcgcagc gtgacttcgc cgttaaacaa acgggcgacc
     2701 tcactgacaa acgccaaccc cagaccgctg cttttttgcc catttgcacg cggcaaagag
     2761 taaaagcgtt caaaaatacg tgacagcgcg tagtcaggaa taccactacc ggtatccagc
     2821 accttaaggg cgacgtgttc ctgatccact tcggcgctta gcgttatgcg accgctcttg
     2881 ggggtaaaat cgatggcgtt atccagtaaa ttccccagcg cctgctccag taacgccggt
     2941 tcagcagcaa cgttaacctc agtaggcata acatgcaaag tgatgttttt ttctgccaac
     3001 tgcacggtgc gcgcttcgct gacgcggcga aataatgccg ccacatcaac agcagtcaga
     3061 acgacttcct gacgattctc cagtcttgcc tggcgtagta acgtttctac caatgcctgc
     3121 atacgcgcat tttgcgtcag aatgttgtcg gtaaaacgag ccaccacttc cggtggcgga
     3181 ccttcgcgta aaatttccgc cgcgccacga atcgccgcca gtgggctttt tagctcatgg
     3241 gttaacgcat aaacatactg ctcaatatag tttttccctt ccagcttcac gcgcatactt
     3301 tccagcgcct gcgcaagctt acgcaactcg ctactgccga gttcaggaag aggaacgggc
     3361 ttattgtcag tgacggaatc ggcatagcga gtgagccggg caatggagcg gttgatccac
     3421 caaaccatgc ctgcgccaat caccagtgca atccccagca aaatggcgct ggcccataaa
     3481 attcgccgct cgctgcgctt aatgaccgga gccatcgccg cgttcggttt gcctacgctc
     3541 aaaacgccaa taagccgcga gccgtccata atcggcgcgg caacatacat caccgaactt
     3601 tcgggatcgg caggattttg caacgtgctg cgcgcaccat actgaccacg caacgttagc
     3661 cagacgtcat tccagcgcga ataatcctgc ccaacggctt tatttgccga atcgaacaat
     3721 actttgccct gcgcatcggt catatagaca tgatattcat tgcgcacttt gttaatgcca
     3781 ccgatattag cgcgaaacgg gcgatgttgt agctgattaa acgcctgcgc cagttgccca
     3841 tgcgttgggt ccccagagag caaatcggga cgcgccagct ccgccagcaa cgttgcggtg
     3901 tcgatcagcg tcccctccgt tgctcttcgc acgcccggtt taacttcttt gacaaaaata
     3961 gccagtacga accaggccgc caccgccacc agtaaaaaat agcccagcaa caaccgcatg
     4021 ccgatacgca ttacaggctc ctcaggctgt atcccatgcc gcgatgagta ttaatcggtg
     4081 aaagatcggg gttgatggcg cgcagcttgg cacgcagcgt tttaatgtgg gtatcgacgg
     4141 tgcgatcgta ggtgtcctgc gcatcttccc acacgatatc catcagttgc tggcgggacc
     4201 agacgcggcc tggcgacttg agtaacgtct tcagcaataa aaactcatac cgcgtcagcg
     4261 ttaatggcgt gtcaaaccag ctgatctgcg ccgcgggttc attcaattca aaatgaccaa
     4321 tacggatgac gggagacggc gtcgagaact tcttcacccg acgcagcaag gtgcgcaccc
     4381 tagcgcacac ttcgcggggt gaaaacggtt tagccacata gtcgtcagcg ccaatttcca
     4441 gcccaagcag gcgatcgacc tcttcacttc gggccgtcag gaacagtaca ggtaacgccg
     4501 gatggagcgc cagtaactgg cggcacaatt caaagccact gatatcaggc aaaccaacgt
     4561 cgagaatcat gacatcggga acctgctggc gagctttatc cagcaccggc aagcctcgct
     4621 caaagacctc gacggcaaaa ccttcctgct gcaacatgta gaccagcgtg tcggctatcc
     4681 cttgctcatc ttccactaac cagaccgttt cccgttgcat aaatcgcctc tgttattgtc
     4741 gccacggcat gacaggaacc gcgctaatcg cgtttttcgg cgaaccttct acaactttgt
     4801 cggagtaagc cagataagcg agcgcgttgc gtttggcgtc ataaaagcgc acgacctgta
     4861 acgacttaaa gaccagggac gtgcgttttt tgaatactac ctcgccctga gctttgccgt
     4921 ttttaatacg atccgacagt tcaatcggcc cgacttgctg acaagaaata gccgcatcgg
     4981 aggtatcttc cgccagaccc aatccccctt taataccacc tgttttcgcc cggctcacat
     5041 aacaggtgac atttttcaca tcgggatcgt caaaggcttc cacaacaatt ttgtgatccg
     5101 ggccgatcat tttaaatacg gtgtcgaccg aaccaatctc ttctgcatga gccaatggcc
     5161 ccagcattat caggcttaaa gacaggatca aatgcttgta tttcatattg ttaccatttt
     5221 tatctattac acgagcaatt agttcgtcac ggtaaataac atattgatta agaacaaaat
     5281 ctcaatactt ttatttccgt caagacctaa aacatactct actaagaaaa aaacactgaa
     5341 tgctaaaaca gcaaaaaatg ctattatcca attacctgat gtcaggtgct cgttgttgaa
     5401 aggatgagga tattttatgg atcaggccgg cattattcgc gaccttttaa tctggctgga
     5461 aggtcatctg gatcagcccc tgtcgctcga caatgtagcg gcgaaagcag gttattccaa
     5521 gtggcactta cagagaatgt ttaaagatgt cactggccat gctattggcg cgtatattcg
     5581 tgctcgtcgt ttgtcgaaat cggcggtcgc actacgcctg actgcgcgtc cgattctgga
     5641 catcgcgctg caataccgct tcgactctca acagacattt acccgcgcat tcaagaagca
     5701 gtttgcccag actcctgcac tttaccgccg ttctcctgaa tggagcgcct ttggtattcg
     5761 cccgccgctg cgtctgggtg aattcactat gccagagcat aaatttgtca ccctggaaga
     5821 tacgccgctg attggtgtta cccagagcta ctcctgttcg ctggagcaaa tctctgattt
     5881 ccgccatgaa atgcgttatc agttctggca cgattttctc ggcaacgcgc cgaccattcc
     5941 gccagtgctc tacggcctga atgaaacgcg tccgagtcag gataaagacg acgaacaaga
     6001 ggtattctat accaccgcgt tagcccagga tcaggcagat ggctatgtac tgacggggca
     6061 tccggtgatg ctgcagggcg gcgaatatgt gatgtttacc tatgaaggtc tgggaaccgg
     6121 cgtgcaggag tttatcctga cggtatacgg aacgtgcatg ccaatgctca acctgacgcg
     6181 ccgtaaaggt caggatattg agcgatacta cccggcagaa gacgccaaag cgggagatcg
     6241 cccaattaat ctgcgctgtg aactgctgat tcctatccgc cgttaacgct gcagctcatc
     6301 taatgcaggg gcgtccagat gcgagatgtc ccctgcggtt tcaacgaccc agccagacgc
     6361 cagccacagg ctttcctgat aatccacgcg cgaaatagaa cagttacgca gacgtaagcg
     6421 gcgctctgcc catgctggta atccgagaat cgtactcacc agacatccca gtgcaatacc
     6481 atggctcacc agcagcggtc ggcttccctg cggtaagtcc cggcaagatt ccagtgccgc
     6541 attgacgcga tcgctgagct cctgcattga ctcgccttca ggaatacgcc catcaacggt
     6601 gccattgacc agctgccgac gccagttctc ttcttcttcg gtcagagaat cgatatgtct
     6661 tttttccagc acacccatgt ttaattcacg caggcgagaa tcaaagatga tgtcacagcc
     6721 gcaggcctgg gcgatgattt ccgccgtacg ccgggtgcgt cctaaatcgc tactgatgat
     6781 atgcgtaatg ccaagctctt tggcacgcgt cgccacctgc atcgcctgtt gctcaccttt
     6841 ggcggtcagc gggctgtcag actggccctg aatacgtcgc tcggcgttcc actgcgtttc
     6901 accgtggcgg actaggtata cctgtaacat gctttttttc cgtatactgc tgtaacgtta
     6961 attttctgag taatgctgat tatgcaccaa gttgtctgtg cgaccaccaa tcccgctaaa
     7021 attcaggcca ttctgcaggc atttcacgag atcttcggcg aaggatcctg ccatattgca
     7081 tccattgccg tcgagagcgg tgtaccggaa cagccctttg gcagtgagga aacgcgcgct
     7141 ggcgcacgaa accgggtagc caatgcccgc cgtttacttc cagaggctga tttttgggtg
     7201 gcgattgaag ctggcattga tggcgacagc actttcagtt gggtggtgat tgaaaacacc
     7261 acccagcgcg gcgaggcgcg ttcggcaact ttgccgttgc ctgcggtgat attggaaaaa
     7321 gtacgtgaag gtgaggcgct tggtcccgtg atgtcgcgtt ataccggtat tgatgagatt
     7381 ggtcgtaagg aaggggcgat cggcgtgttt accgccggga aactcactcg cgccagcgtc
     7441 tatcaccagg cggtgatcct ggcgctgagt ccgtttcata atgcggtgta ttaagcgcct
     7501 gatgcgacgc tgccgcgtct tatcatgcct accaaacata ttgaaattac gggtatttgt
     7561 aggacggata aggcgttcac gccgcatccg gcattcggtg cacgatgcct gatgcgccac
     7621 gtcttatcag gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag ccactggcgc
     7681 agctccacgg gcgcggcttt caggctgtta gatccacgcg taatcgtcgc gatgcctgcg
     7741 ccgagttcat tttttaactc acgctggctc atttcgccgc gcaacagctc ttcgacaata
     7801 cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt taacaacggt
     7861 aaatggagat cgttttggta ggcattctta agcaggtcga caaaacgtaa ccactcctgg
     7921 tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt gggccataat atgtcgccat
     7981 tgttagcggg ggaagcaaaa tgcctccccc ggttgtactc gctaaagagt acgatagcat
     8041 atcataaacg tgcggatcag taacgacgtc cccattccgt ggcgctcatc aacgtcggtt
     8101 tatcccccat gaaatagcgg tagtaagcgt cataagccag cacgttcttc acataaccgc
     8161 gcgtctcgga gaacggaata ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt
     8221 tgccaagcca ggttcgcacc cgtccaggtc cggcgttata agctgctgag gagaaaatac
     8281 ggttattacc aaattgctga taaacatatt gcaggtaact ggtgccaatg ttgatattcg
     8341 tttccggatc cagcaattgc ccagggctgc tataaccagg aatagagaac atcttcaccg
     8401 tatgggtcgc cgtgcccggc ataatctgca tcaggccgct ggcccctacc ggtgatttca
     8461 ctttcggatt ccaggcgctc tcctgacggg caatcgccat cgcatagctt tgcgggatct
     8521 ccttcccact ggtgtaacgt ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca
     8581 gatgatccca aagcttcccg gcgatcgttg cctgaacgct aagatcccac cattggttgt
     8641 tgaaagcata ccgcgccagt tgagcctgct ctgtttttga cttgctcttc accagattgg
     8701 cccactcgct acgcgcggtg ttatcgagat tccagtacat caactcgcgc acgcgcgcca
     8761 tctccggccc ctgagtcagg gcgctgtcaa cattctgcgg cgctttatca atcttcagct
     8821 catactcttc gccgatgcgt tgtgcagcaa ccatcgggta gaaaccacgc tgttgcatga
     8881 gttgatgcaa aatctctttt gcttcagctt cacgtccgcg ttccagcaat aaatccgcct
     8941 gccagtaacg ccattcatct ttctctttcg cttccatagg cagacgcgcc agccaggtat
     9001 tcaggccgcg acgatcgcca gtgccaagcg ccattcgcac acggcgttca ataagcgaag
     9061 tagattgcga gcgcataatg gcgtcatcgc gccatttcgc ctgctcgtcg gtgacatcgt
     9121 tgcccatcaa acgccaggcg acgatatcgc gcagctcctg aatctgatct tcattaagct
     9181 gctgcgcctg ggcaagcgat gggatcatca gccgtgcatt ttcagcatcc tgccgcgcca
     9241 cactggcaaa cgccaccgcc gccatttgac gggtaaaatc ggtcgctccg gttgtacgcg
     9301 cgaaggtcag taccgtattg gggttgttcg ccagtgaaat gattgccgag gcgatagtct
     9361 ggtaatcggc aggcatctgc cctgccagca ctgttaccag gccagtgtta cccgctttca
     9421 tcgccagacg gatacgctct aaatacgcca gcggatcttg tttacctgac gcacgccaga
     9481 cgctaaataa cttgtcacag gcgttaggct ggctcttgcc ggttagccac agctctttcg
     9541 ccccttgcca ggcttcttca ctctgcccgg tgttccattt cgcatagtag taattacatt
     9601 gtgcttcggt agttccgggc ttttccgggc taaaggctaa caggccacgc cagtcttcac
     9661 gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg agcgggagga agcgtggggt
     9721 tagcgcgaac aaagttagtg accgtcaccg ccggttgatt catcaggtca tcggtgatct
     9781 ggcggtattc caggtaggga taaagcggat aatccttcag tccaggcatc atttgttcga
     9841 ccacatccat ttgtcgatta tcccaggcct gcttgatttg cgcgtaacga ctacgctgct
     9901 catccagtga gtcggctcgc gccacgctgc tgaccgtcag cagacagaca ccggcagcca
     9961 acagccgcca ggtaacttgt ttggcttttt ccacaagcgc atcctctaag tgtaaataca
    10021 tcaatgcagc gtcatgccgc gtaatagatt tatgctaacc agtcattgcc gtttacgcca
    10081 cgttacggac acttttttac ttttactgcg agggtgatcg accggctggg attaggcggg
    10141 gaaaaaggct acacttcgcc tttgaaaact catcatcacg ataaacatag aggcgaagtc
    10201 caacgtggct caattcgttt ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg
    10261 tcatattttg aaaaacatct ctctgagttt cttccctggg gcaaaaattg gtgtcctggg
    10321 tctgaacggc gcgggtaagt ccaccctgct gcgcattatg gcgggcattg ataaagacat
    10381 cgaaggtgaa gcgcgtccgc agccagacat caagattggt tatctgccgc aggaaccgca
    10441 gctgaacccg gaacacaccg tacgtgagtc cattgaagaa gcggtttcag aagtggttaa
    10501 cgccctgaaa cgcctggatg aagtgtatgc gctgtacgcc gatccggatg ccgattttga
    10561 caagctggcc gctgaacaag gccgtctgga agagatcatt caggctcacg acggtcataa
    10621 tctgaacgta cagctggagc gtgcggcgga tgcgctacgt ctgccggact gggacgcgaa
    10681 aatcgctaac ctctccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga
    10741 aaaaccagac atgctgctgc tcgacgaacc gaccaaccac ctggatgccg aatccgtggc
    10801 ctggctggaa cgcttcctgc acgacttcga aggcaccgtg gtggcgatta cccacgaccg
    10861 ttacttcctc gataacgttg cgggctggat cctcgaactt gaccgcggtg aaggtattcc
    10921 gtgggaaggt aactactcct cctggctgga gcagaaagat cagcgtctgg cgcaggaagc
    10981 ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg
    11041 aactaaaggc cgccagtcga aaggtaaagc acgtctggca cgctttgaag aactgaacag
    11101 caccgaatat cagaaacgta acgaaaccaa cgaactgttt attccacctg gaccgcgtct
    11161 gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc tatggcgatc gtctgctgat
    11221 tgatgacctg agcttctcga tcccgaaagg agcgatcgtc gggatcatcg gtccgaacgg
    11281 tgcgggtaaa tcgaccctgt tccgtatgat ctctggtcag gaacagccgg acagcggcac
    11341 catcactttg ggtgaaacgg tgaaactggc gtcggttgat cagttccgtg actcaatgga
    11401 taacagcaaa accgtttggg aagaagtttc cggcgggctg gatatcatga agatcggcaa
    11461 caccgagatg ccaagccgcg cctacgttgg ccgctttaac tttaaagggg ttgatcaggg
    11521 taaacgcgtt ggtgaactct ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct
    11581 gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg atatcgaaac
    11641 cctgcgcgcg ctggaaaacg ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca
    11701 cgaccgttgg ttcctcgacc gtatcgccac gcacattctg gattaccagg atgaaggtaa
    11761 agttgagttc ttcgaaggta actttaccga gtacgaagag tacaagaaac gcacgctggg
    11821 cgcagacgcg ctggagccga agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa
    11881 atgccggatg cggcgcgaac gccttatccg gcctacaaaa ccagcaattt caatacgttg
    11941 taaaactgta gacctgataa gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc
    12001 cggatgcggc gtgaacacct tttccggtct acaacagcct tagcatcctc aatttcgtgg
    12061 cgggccaccc gtgcaggttt tttcaaacct tgtttaatct cgacagcctc ttccagagag
    12121 gttttcaact catcaaaaaa actcatttta tacctcgcct ttgagcagtt tcatcacggt
    12181 tatctctgct cccccatcat ctcccgcacc agctccacgc agcgcaggaa acggctgtcg
    12241 taatcttcct cttcaacccg cacaaattcg atattgttct cttccagcat ctccaccagc
    12301 agattctgga actctttacg atccaccgaa ctgccgaggc tgcgtaaacc atccaccacc
    12361 cacggcgtgt tgttctccag caggatcacc agatcgaaac ggtattcatc aatcagcgcc
    12421 tgcacgaacg gatgttcccg cccttcgtac tttttgcaga acgcctgagt ggtgacaaaa
    12481 tcggtatcga taaatgccac tttattggca tatttcaccg caaaatcaat gtattgagcg
    12541 tggcccagcg cgattttatc gtagtcagaa tactgcaatg cgatctcatc accgccgagg
    12601 tgtgaaaaga cataatcgcg gccatattcc cacgcactgg tggtgttgaa gatattggca
    12661 agtttgttta ccagggtgga tttaccgctc gactcgccgc caaggatcgc cacggtacgc
    12721 acaaaaaacg gcttcacttc ggtaggaata tattcccagt agcggaacgg gttttcgcgg
    12781 atctgcgcac cgctgatact cataaaggta cgtttcggat cgaccagcac cgtctcgatc
    12841 cccagatgtt ccatatactg tggcgcatcg gcttcttccg aggtgtagat cagatccggc
    12901 tggatcccct tttcagccat aaactttttg ataccgttgc tccacacatc ccagccgtgc
    12961 ggatacggct ccatgccctc ttcgttgaaa gcatgaatgc gaatattttt ctgatattta
    13021 aaagtttgca ataaccagcg cagacgatcc ggcacggtcg gctgctgcga catggcactg
    13081 tcttcgaaca acgcgcggtc acgggtatcg tcaaaaccca taatgatatg cagctcgtca
    13141 acctggctac aggcgcgctg gataaggtag atatgtccgg tatgcagtgg gtagaactta
    13201 ccgaatacga caccgatcgt tttcttctgc cggggaaact caagccccaa aaaacggtgc
    13261 aacgcctcca gcttttgcgc gctggggctt ttgattttgg cattcagtaa ctggcttaaa
    13321 taccctttgg tcataccgct ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg
    13381 atggcagttt tcaggtaatc aaatgacgac atatctccct ccgtatttct cattataagt
    13441 cgtcgaacac gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc atcccttccg
    13501 gcgctttttt cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg
    13561 cttcagagat tcgttcctga ccgctgggca ccgggcggat ctcccctgcc agcccgactt
    13621 caccaaacac caccagatcc tgcggtagcg gtctgtcacg caggctggaa accatcgcca
    13681 gcagtaacgc taagtcggcg ctggtttcgg ttacctttac gccgccgacc acgttcacaa
    13741 acacatcctg atcggccatt tgcagaccac cgtgacggtg caagacagcc agcaggattg
    13801 ccagacggtt ttgttccagc cccactgcca cgcggcgcgg attcgccatc atcgagtgat
    13861 cgaccagcgc ctgaatctcc accagcaacg gacgcgttcc ttcccacacc accatcacgg
    13921 agctgccgga ggtcacttca tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt
    13981 cacgcagccc ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt
    14041 ttttatggct gcgcaaggtg cgaaaacggg agtcggcatc gccatctaaa agcaccgaac
    14101 agtcgataca gtgttccagc actttcgggc cagccagcga accatctttg gttacgtgcc
    14161 ccaccatgac aatcgccaca ccgcgcgttt tggcgaagcg tgtcaaataa gccgccgttt
    14221 cacgcacctg cgccacgcta ccaggcgatg actgtacatc cgccatatgc atcacctgga
    14281 tcgagtcaat taccatcagc ttcggttgct cttcttcggc aatcaggcag atctgttcga
    14341 tgctggtttc cgacaacata ttgagattgt cagtcggcag gccaaggcga tgagcgcgca
    14401 ttgccacctg ttgcagcgac tcttcgccgg tgacatacag cgttttcatc tgctgggcca
    14461 gtttgcacag cgtttgcagt agcagcgtgg atttccccgc accagggtta ccgccaatca
    14521 gaatggcact tcctggcacc acgccgccgc ctagtacgcg gtcgaactct ttaaatccgg
    14581 tggaaaaacg cggcagctct tcaaggctga tatcggagag tttctggact tttgccaccc
    14641 cggcgctacc ggcatagccg ctgagacgct cgttacgcgc caccgtaggc gacgcagcaa
    14701 gacgcacctc ggtgatggtg ttccaggcat gacaggcact gcactgcccc tgccagcgcg
    14761 gataatcggc cccgcattca ttacaaacaa aggcgcgttt tggagctttt gccacggtgt
    14821 acctcgttaa tgctgtgccg cccgcaggat ggcgggcgag caattacttc tgattcaggc
    14881 tgcctgagag gatgcagaat acccccatca ggtcagcgtg acggatggtg acttccgcct
    14941 tttcattcac ttttggcttg gcatggtagg caatccccag ccctgccgct ttgatcatcg
    15001 gcaggtcatt ggctccatcg ccaatcgcca cggtctgcgc cagcgggatt tcatactcct
    15061 gcgcgaggcg agtcagagtt ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat
    15121 tgccggtaaa tttaccgtcc atgatctcca gttcattggc taccacggct gtcaggcgca
    15181 gcttgtcgcg caggtattca gcaaagaaag taaagccgcc ggaggcaatc gccactttcc
    15241 agcccagcgt ttccagcttg agcaccagtt gcgttaagcc tggcatcagc ggcagatttt
    15301 cacgcacctg ttgcagaata ttggcgtcag cgcctttcag cgtcgccacg cggctgcgca
    15361 ggctggcggt aaaatcgagt tcgccgcgca tcgcccgttc ggttacttcc gccaccatct
    15421 cgcccgttcc ggccagtttg gcaatttcat caatacattc aatctggatg gcggtggaat
    15481 ccatgtccat caccagcaaa cccggcgtgc gcaggtgcgg gatttttccc agcggcgcga
    15541 catccagctg cgcttcgtgg gccaggcgtg tagcccgtgc ggtgagtgaa cctgccagac
    15601 gaatcacctg ataatcttcc acgcaccagg cggcaacaat caccatcgcc gcacccagtt
    15661 tgctctggta ttgggtcaga cgctgtttat ccagcccacg accatacagc agccagccgc
    15721 tacgacctgc gtggtaatcc agtggcatca cttcatcacc acttaatgaa agaggcagac
    15781 ccggccataa agagacatct tcaggcaggt cgcaccaggt aatgttaggc attaaggctc
    15841 ctgtaaaatc gttcgaagca gggaaaataa cgcatgaggc taccttgtat ccattgcttc
    15901 tggcaacatt aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt
    15961 ccggctgcat cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg cactgatgca
    16021 gggggcgtca tggtttagtc aaaaccacca gcgacagcgt aatccacagc tggaagaact
    16081 ggcccgcacc ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc gtaccgactc
    16141 accggatgaa aaacgcattc aggcgatcct cgatcagtta acggatgaaa gccgtatcct
    16201 cgacgcgggt gtgtatgacg aacaaggcga tcttatcgca cgttctggcg aaagcgtcga
    16261 agtgcgcgat cggctggcgc tcgacggtaa aaaagcaggc ggctatttta accagcagat
    16321 tgtcgagcca attgccggga aaaacggacc gctcggctat ctgcgcctga cactcgacac
    16381 ccatacgctc gccaccgaag cccaacaggt ggataacacc actaacattt tacgcctgat
    16441 gttgctgctc tcactggcaa tcggcgtagt gctgacccgc acgctgctac agggtaaacg
    16501 cacccgctgg cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc cggaagagga
    16561 agaaagcgag aaaaaagagt gccccattac tacaagaaag gaaatcgtta tgtccacatt
    16621 acgcctgctc atctctgact cttacgaccc gtggtttaac ctggcggtgg aagagtgtat
    16681 ttttcgccaa atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt
    16741 agtaattggt cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga
    16801 taacgtccgc ctggcgcggc gcagtagcgg tggcggcgcg gtgttccacg atctcggcaa
    16861 tacctgcttt acctttatgg ctggcaagcc ggagtacgat aaaaccatct ccacgtcgat
    16921 tgtgctcaat gcactgaatg cgctcggcgt cagtgccgaa gcgtccggac gtaacgatct
    16981 ggtggtgaaa accgccgaag gcgaccgcaa agtctcagga tcggcctatc gcgaaaccaa
    17041 agatcgtggc ttccaccacg gcaccttgct gctcaatgcc gaccttagcc gcctggcaaa
    17101 ctatctcaat ccggataaaa agaaactggc ggcgaaaggc attacttcag tgcgttcccg
    17161 cgtgaccaac ctcaccgagc tgctgccggg gatcccccat gagcaggttt gcgaggccat
    17221 aaccgaggcc tttttcgccc attatggcga gcgcgtggaa gcggaaatca tctccccgga
    17281 caaaacgcca gacttgccaa acttcgccga aacctttgcc cgccagagta gctgggagtg
    17341 gaacttcggt caggctccgg cattctcgca tctgctggat gaacgcttta gctggggcgg
    17401 cgtggaactg catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tgtttaccga
    17461 cagcctcaac cccgcgccgc tggaagccct cgccgggcga ctgcaaggct gcctgtaccg
    17521 cgcggatatg ctgcaacaag agtgcgaagc gctgttggtt gacttcccgg accaggaaaa
    17581 agagctacgg gagttgtcga cgtggatagc gggggcggta aggtaactcg ctgattaaaa
    17641 tatccaagga aggaaatatg atcgatctgg aaaaccagga acgggagatt atcaatttaa
    17701 tgttcagcca gcgtatttcc tggctagccg ccgtgcgtat caggcataaa ttatcgcttg
    17761 ctgaagtaag taaaatgtta ggtatctcga ttaattctct aaaacagatc gaaaagacgg
    17821 aacggcttag ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt ccaccagagt
    17881 tgctgatatg cccttcctgg atgacggctg aacataaatg aatagattcg tgaatagata
    17941 cttgaatgga
//
LOCUS       DAZFDC010000095        17664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFDC010000095 DAZFDC010000000
VERSION     DAZFDC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.095"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..206)
                     /locus_tag="ACHJ48_003355"
     CDS             complement(<1..206)
                     /locus_tag="ACHJ48_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4708842.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDA"
     gene            complement(190..513)
                     /locus_tag="ACHJ48_003356"
     CDS             complement(190..513)
                     /locus_tag="ACHJ48_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4708843.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            638..1114
                     /gene="racR"
                     /locus_tag="ACHJ48_003357"
                     /gene_synonym="rarC"
     CDS             638..1114
                     /gene="racR"
                     /locus_tag="ACHJ48_003357"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFO4708844.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFLLSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1430..1582
                     /locus_tag="ACHJ48_003358"
     CDS             1430..1582
                     /locus_tag="ACHJ48_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4708845.1"
                     /translation="MDTLDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LARNSD"
     gene            complement(1697..2212)
                     /locus_tag="ACHJ48_003359"
     CDS             complement(1697..2212)
                     /locus_tag="ACHJ48_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708846.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGTYQEINASLKAKIAEFENFEAQTEGYILSQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            2336..2734
                     /locus_tag="ACHJ48_003360"
                     /pseudo
     CDS             2336..2734
                     /locus_tag="ACHJ48_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            2796..3065
                     /locus_tag="ACHJ48_003361"
     CDS             2796..3065
                     /locus_tag="ACHJ48_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4708847.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            3034..4152
                     /locus_tag="ACHJ48_003362"
     CDS             3034..4152
                     /locus_tag="ACHJ48_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4708848.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGVIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            4319..5113
                     /gene="potC"
                     /locus_tag="ACHJ48_003363"
     CDS             4319..5113
                     /gene="potC"
                     /locus_tag="ACHJ48_003363"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO4708849.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            5110..6156
                     /gene="potD"
                     /locus_tag="ACHJ48_003364"
     CDS             5110..6156
                     /gene="potD"
                     /locus_tag="ACHJ48_003364"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO4708850.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLSPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(6312..7133)
                     /gene="cobB"
                     /locus_tag="ACHJ48_003365"
     CDS             complement(6312..7133)
                     /gene="cobB"
                     /locus_tag="ACHJ48_003365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO4708851.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(7149..8060)
                     /gene="nagK"
                     /locus_tag="ACHJ48_003366"
     CDS             complement(7149..8060)
                     /gene="nagK"
                     /locus_tag="ACHJ48_003366"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO4708852.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLPAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(8089..9333)
                     /gene="lolE"
                     /locus_tag="ACHJ48_003367"
     CDS             complement(8089..9333)
                     /gene="lolE"
                     /locus_tag="ACHJ48_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO4708853.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(9333..10034)
                     /gene="lolD"
                     /locus_tag="ACHJ48_003368"
     CDS             complement(9333..10034)
                     /gene="lolD"
                     /locus_tag="ACHJ48_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO4708854.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPWLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(10027..11226)
                     /gene="lolC"
                     /locus_tag="ACHJ48_003369"
     CDS             complement(10027..11226)
                     /gene="lolC"
                     /locus_tag="ACHJ48_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO4708855.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            11488..12561
                     /gene="ycfT"
                     /locus_tag="ACHJ48_003370"
     CDS             11488..12561
                     /gene="ycfT"
                     /locus_tag="ACHJ48_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO4708856.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAALAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPVAVSYSR"
     gene            12689..16135
                     /gene="mfd"
                     /locus_tag="ACHJ48_003371"
     CDS             12689..16135
                     /gene="mfd"
                     /locus_tag="ACHJ48_003371"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO4708857.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMEKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     LSRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            16279..17241
                     /gene="ldtC"
                     /locus_tag="ACHJ48_003372"
     CDS             16279..17241
                     /gene="ldtC"
                     /locus_tag="ACHJ48_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO4708858.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(17324..17581)
                     /gene="bhsA"
                     /locus_tag="ACHJ48_003373"
                     /gene_synonym="ycfR"
     CDS             complement(17324..17581)
                     /gene="bhsA"
                     /locus_tag="ACHJ48_003373"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO4708859.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4510 a         4524 c         4408 g         4222 t
ORIGIN      
        1 gcatcagggg tgtctttatc cagccaacgg aaaattttct gggtattacg agccagggct
       61 tccggatggc tgtcgtcgta cagttccggg aacgtcatcc ccagttcgaa ataagtccgg
      121 gctatttcag ctgcaggaac tttctcacca tcaggatatg cccaggcatt catcgccatg
      181 cggatgtgct catgtttgat tttcatgaat catttgcctc ttgatgcttc gggtatgatc
      241 gttttcgtca tttggttgct tcatcgacat attctgcgaa taacatgacg agcgtcgtaa
      301 gtatgtccaa tcaacatcag gacgaagttc ttcacacagg acaccacctt ttgttgctcg
      361 ttcaatcgca ggacatctct cagcaggcaa ctgacgtaca cctttgatcc attgatttac
      421 gcttggagga gatacaccta aaagcctagc cattgctgat tgcccaccga caacagcaca
      481 agctcgtttg aatgaatagt tatctttttt catcgaatga actccaaaaa acacacaata
      541 atattaggct tagcctaatg caattgtcaa taggctatgc ctaacgcttc gagagtaggt
      601 attgcctaat gcgatgcgca taggagacta ttaagcaatg cttagtggta aagacttagg
      661 ccgagcgata gagcaggcca ttaacaaaaa aattgcatca ggagccgtca aatcaaaggc
      721 ggaaatcgca cgtcatttca aagtccaacc accatcaatc catgactgga ttaagaaagg
      781 ttcgataagt aaagacaaac ttccagaact atggcgtttc ttttctgatg tggttggtcc
      841 agagcattgg gggcttaacg aataccccat accaacccca tccacttcag atacaaaaag
      901 tgaactttta gacataaaca gcctttatca agccgcctct gatgaaaaaa gagcaattgt
      961 ggctttcctc ttatctggaa atgctacgga gcctagttgg gttgatcatg acgttcgtgc
     1021 ctacattgcc gcaatggaaa tgaaggtagc taactatctg aaaaatcaag aatcaaaacg
     1081 gaaaagccag aacatcacca agacaggaac ttaaacttat atggtccgac gggaaattcc
     1141 tagttcccgt tagttaactc ctactacctc tcccacaaac catcacctat taggtcgcac
     1201 ccaaatcatt aggcgtagcc tattgacaag caattaggca tttcctatag ttttcccata
     1261 ccaacccatc ccgtcccaca caatacaggg caatacctag agttacccgg cagtggtcag
     1321 ggattaagta gccagcccga ggcgtaagaa catgacggca gggttcaact ttaactatgc
     1381 agcaggtttt tgttccgcta ccccggcgtt aaggggatat gaggtcagca tggatactct
     1441 tgatcttggc aacaacgaat ctctggtgtg cggcgtgttt cccaaccagg acggcacgtt
     1501 caccgcgatg acgtatacca aaagcaaaac gtttaaaacc gaatctggcg cgcgtcgctg
     1561 gttagcaaga aactctgact aatgaaatct ggtaattaag gaatcctcca cggggggaga
     1621 gtggtgcaca cgcgccggac acaagcaagc atccggcatg ctctttaaca atctggatat
     1681 ccataacagt aaaaatctac agattgccgt tcagttttct ggccaactct tcaatagatg
     1741 gaggtgatac gtaatccgga tttttattca taagaaactg attttcacag tacaggcacc
     1801 tgcttttatg aaaaaattca tattctctaa ccgggaatgg ctgaagtatc gatactatct
     1861 tttgtccaaa acattttgga caaagatgca tgattatgct gccgccgttc acggttacct
     1921 ccttcgagta cacaaaagtt cccgactcaa gttggctaag gatataacct tccgtctgag
     1981 cctcaaagtt ttcaaattct gcaattttag ctttgagaga agcatttatt tcttgataag
     2041 tgcccaccag ctcaacgaga gacacgcatt cgcgctgaat agatgcaagc tttgagttca
     2101 gttcaccaat agccgcattt acttcagctt gagtttttgc ctcgttcatt agttttgcaa
     2161 tctgggcggt ttcacgaata gccgtcattg ctgccgttaa ttcagcgatc acattcaata
     2221 ctcttattgt tgttggggat atccagatta acaagatcct tgttgttggg gaataacggg
     2281 gaccacctcg cctgacgtgg taaaaagcag gcacacaaca cgaaagcaca cggcgaggga
     2341 caccattaaa tggtggctta agcaatcacg cgaagcgcaa tctgccatta tgaccgatga
     2401 aatcccgtta gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga
     2461 attttttgtt caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc
     2521 atacgaacgg caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac
     2581 aatcgttgag ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg
     2641 tgagcgccat aatgcacttg atgacgcccg ttaccaggca aaatacgttt cagttatctg
     2701 gcaaaaactg atcccgagtc aggctgattt ttaatgttca accgtcgcca gttgtcgttg
     2761 atattctgca actggcgcgt tccggagtga tagccatgag cgaacagtac ctgataacgc
     2821 tcgacgagtg gaaaccaaaa cggttcagtc tcccaataac aaacactacc ctggtgaaat
     2881 acggaaaact aggatacatc gttccaagac cacaaaaaat tcgtgggcgt tggctgatag
     2941 atcgccgagc agtatttgtt gggcctggtg aaacgggaat tgcgccggaa attcatactg
     3001 gcgatgatga tgcactgaag gagattttaa ctcatgtcac cgaggccacg aaaaaacagc
     3061 actgacgtag ccggtcttta cgaaaagttt gatcgcagaa ctggcagagt ttactaccag
     3121 tataaaaatc ctgtgactgg aaaatttcac ggactcggaa cagacaaagg taaggcagaa
     3181 aaaatcgctt ccacagccaa tcagcgaata gctgcagcag aagctgaata tttcatgcgc
     3241 aaaattgatg aaagtccgtc agcaacaaaa cgtcggggta tcagattaaa ggcatgggtt
     3301 gatcgatatc tgaaaataca ggacacgcga ctgaaaaatg gagatattgc agctacaact
     3361 cacaaagaaa aaactcgaat ggctgcatac ctggtttccc gtctgggaaa ccacccattg
     3421 aaagaactgg aagtaagaga ctttgcatta atactggatg agtggctgga taaagacatg
     3481 gtcagtacag cgagagtaaa tcgtggatta tgggttgata tttataaaga agcacagcat
     3541 gcaggggaag ttcctcctgg atggaatcct ccggaggcta cccgtaaacc gatccctaaa
     3601 gtaaccagag ccaggctcac catggaagac tggcaaaaaa tttacaatgc aacgcctgaa
     3661 aaacacttta tccgtaacgc aatgcttctt gcgattgtta ctggtcagcg ccgtgatgac
     3721 atttgccaca tgcgtttttc agatgtgtgg aacgaacact tgcatatcac ccagggaaaa
     3781 accggaatgc gtctggcgtt accgcttaca ctacgctgtg atgccattgg gataacgtta
     3841 aaagaagtta ttgatgggtg ccgagacaga atattaagtc catatctaat ccatagtcgg
     3901 caccagaaac aaccgaagcc gatgagtaaa gacaacctga gcgactactt tgccaaagca
     3961 cgggatctgg ctggggtaat tccaccagca ggaaaaactc cgccaacatt tcatgaacaa
     4021 cgctctctat cagaacggct gtaccgtgca cagggtatcg atacaaaaac attactagga
     4081 cataaagtcc aggcaaccac cgatcgctat aacgatactc gaggtcagga atgggttaag
     4141 ttggttattt gacaaacacc atatggaata tccgtcacca tctcaggaaa aaagtgttgt
     4201 aaaatgcgtg gcctagtttt ggggaaacgt tttggagaga ttttggagaa aggaaaaaat
     4261 tgaataaatt caaaatcctg aaagtgtccc gccttatgaa taaaaaggtg gaactcgaat
     4321 gatcggtcga ctgcttcgcg gcggttttat gaccgctatc tacgcgtacc tgtatatccc
     4381 aatcattatt ttgattgtga actcctttaa cagctcgcgc tttggcatca actggcaggg
     4441 ttttaccacc aaatggtata gcctgctgat gaacaacgac agcctgttac aggcagcgca
     4501 gcattcacta acaatggcgg tgttttcggc gacgtttgct acgcttatcg gttcactgac
     4561 ggcagttgcg ctgtaccgtt atcgctttcg tggtaagccg ttcgttagcg gaatgctgtt
     4621 tgtggtgatg atgtcaccag atatcgtgat ggcgatttcg ctgctggtgc tgtttatgct
     4681 gctgggtatt cagcttggct tctggtcgct gctgttctcg catatcacct tctgcctgcc
     4741 atttgtggtg gtgacggtgt attcgcgcct gaaaggtttt gacgtgcgga tgctggaagc
     4801 ggcgaaagat ctcggtgcca gcgaatttac cattctgcga aaaatcattc tgccactggc
     4861 aatgccagcg gtggcggcag gctgggtgtt aagctttacc ctgtcgatgg acgatgtggt
     4921 ggtttcttcg ttcgtcaccg ggccgagtta tgaaattctg ccgttaaaaa tttattcgat
     4981 ggtcaaagtc ggcgtatcgc cggaagttaa cgcgctggca accatattac tggtgctgtc
     5041 gctggtgatg gttattgcca gccagcttat tgctcgtgat aaaacgaaag gtaacacagg
     5101 ggacgttaaa tgaaaaaatg gtcacgccac ctgctcgcgg cgggtgctct ggcactgggc
     5161 atgagcgccg ctcacgccga tgacaacaac acgctgtatt tctacaactg gaccgagtac
     5221 gtgccgccag gactgcttga acagttcacc aaagaaaccg gtattaaggt tatctattcg
     5281 acttacgagt cgaacgaaac catgtatgcc aagctgaaaa cgtacaaaga cggtgcctat
     5341 gacctggtgg ttccttcaac ctattacgtc gataaaatgc gtaaagaagg gatgatccag
     5401 aagatcgaca agtcgaagtt aacgaacttc agcaatctcg atccagacat gctcaataag
     5461 ccgtttgacc cgaataacga ttactccatt ccgtatatct ggggtgcgac ggcgattggc
     5521 gttaacggtg atgcggtgga tccgaaatct gtcaccagct gggccgattt gtggaagcct
     5581 gagtacaaag gcagcctgct gctgaccgac gatgcccgtg aagtgttcca gatggcgcta
     5641 cgtaagctgg gctactccgg taacaccacc gatccgaaag agattgaagc tgcatataac
     5701 gagctgaaaa aactgatgcc aaacgtcgca gcgtttaact ccgataaccc agctaacccg
     5761 tacatggaag gcgaagttaa cctcggcatg atctggaacg gttctgcttt cgttgcacgc
     5821 caggcgggta cgcctattga cgtggtgtgg ccgaaagaag gcggcatatt ctggatggac
     5881 agcctggcga tcccggcaaa tgccaaaaac aaagaaggcg cgctgaaatt gatcaacttc
     5941 ctgctgcgcc cggatgtggc aaaacaggtt gctgaaacta tcggttatcc aacgccaaac
     6001 cttgcggcgc gtaagctgtt aagtccagaa gtggcgaacg ataagacgct ctccccggat
     6061 gctgaaacca ttaaaaatgg cgaatggcag aatgacgttg gcgcagccag cagcatttat
     6121 gaagagtatt atcagaagct gaaagcagga cgttaattca gcaagcgggc ggtggtatta
     6181 ccgcccgttt tttagttagc aaggatgtgg ctaatgagtc aggacagtaa agttttatcc
     6241 cgctctttcg tcggtattgt ggtccgcttg taggcctgat aagcgtagcg catcaggcaa
     6301 tgcttcctgc tttataatcc cttcagcaac ttttcaacaa attctggcac cacctggctt
     6361 gccgggccgt aatatttctc ggcaaattca ttaccgacct gacttggttc aagattcagc
     6421 tccacggtgt gcgcgccatg cagtttcgct tcgtgaacaa acccagccgc cggataaaca
     6481 tgcccggaag taccaatggc aatgaaaata tcggccatcg acaacgccat ataaatttca
     6541 tccatgccga gtggcatttc gccaaaccat actacgtgtg ggcgcaaggg ggccgggaac
     6601 tggcagcaat ggcatttatc ttctggggta acgtctccgg tccagtcgag aacctgacca
     6661 ctttgtgaac aacgaacttt cagcagttcc ccatgcatat gaatcacatt ggtattacct
     6721 gcgcgttcat gcaggttgtc tatattctgc gtcaccagca aaaagcgatc gccgagagca
     6781 tcttgcagtt tagccagcgc aagatgcgcg gcgttaggct gaatttctgg ctgctgcagc
     6841 tgtcgacgac gggcgttata aaacgcttgc accagttcag gatcgcgatc gaaaccttcc
     6901 ggcgttgcca catcttcaac ccgatgttct tcccacaggc catctgcggc gcgaaaggta
     6961 cgaatacctg attccgcaga aattcctgcc cctgtcagta cgagtactct tggtttttcc
     7021 attgcttccg gcaccacttt atctctgaaa aaaatacgct gacgcaaacg ctcgcgcagg
     7081 cggcgtttat ttttacgaaa acgacttaac cgatgacccc gacgcgacag catagcaacc
     7141 tctgttgttt aatcggttag atgtaggaag gccgcaccac gcattcctcc cgcatcaccg
     7201 tggcgcgcgc gttcaatgcg cggaacacga gctacaggta agagatgacg aggcagcctg
     7261 tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc caccaccaat gacgaccagg
     7321 tcagggtcaa caatggtcag gatatttccc agacaaaccg ctaataaatc cagataacgc
     7381 tcaacgtgcg cccttgcctg ctcatcgcct tgatcataaa gcgcaataat ttcgggagcc
     7441 ggcaacggtt gatgataata gtgttgatac agccacgcaa aaccgcgacc agacagataa
     7501 ttttcaatgc agccatgctg accacagccg cagcggcgta acgggaaatc cagccccatc
     7561 atggttaacg catcaaccgg cagacgcata tggccaaact cgccggtaat gtagcttttc
     7621 ccggtaatcg gtttgccgtt gaaaatcagc ccgccgccaa cgccggtgcc gagaatcaac
     7681 cccatcacca acggatattg cgtaaattcg tcatcccagg cttctgaaag ggcaaaacag
     7741 ttggcatcgt tatcaaggcg tacatcgcga tcaagacgtg cgctcaggtc ggcacgcagc
     7801 ggtttaccgc tggcagcagg gacattggcg gcatacagcg taccatcttc tgtttccggc
     7861 attcccggaa taccgatgcc gacagagcct ttacagccaa aacgttgatc agcttcggct
     7921 accagctcgc acactgcatc taaaaatgcg tcatagctgt cacgcggtgt cggcacccgc
     7981 ttttcccact gcaactgccg accgctatca aacacgccaa gcgcaatttt tgttccacca
     8041 atatcaaacc cgtaatacat tgccgctcct tgttttaatg tactgccttt actggccgct
     8101 aaggactcgc gcagggtcaa tattactggc gcgccgcgcc ggataccaac ttgccaaaag
     8161 actcagcaac aatgctgtga ccagtacgta gaagacgtcc agccagtgca attccgatgg
     8221 caggaagtca ataaaataga tatcgctgga gaggaactga tgaccgatca gcttttcaat
     8281 ccgctcaata atcggggtaa gttgcagtga aacaactacg ccgataatca caccacacag
     8341 gctaccgaat agccctgcca gcaatccata ccagacaaag atggcgcgaa ttaaaccatc
     8401 tttcgccccc agcgttctta atactgcgat atcgccactc ttgtctttca ccgccatcac
     8461 taaggtggag acgatgttga aacaggccac gccaatcacc agtaccatcg ccagatacat
     8521 aatggcgcgg atcatctgga tatcgcgata catatagccg taagtaccaa tccagctttt
     8581 aatataaaca tagctgttgg tcacttcacc cgcatcgcgt accagcttat tggcgttgaa
     8641 aacatccgtc attttaaggg caatacctga cacgctggaa cccatatcaa gatattgttg
     8701 ggcatccgcc agcgggatca tggcaaaact gtgatcgagt tgaccactca actgcaaaat
     8761 accggcaacg tgcaaacgca cacgttttgg ctgcatcagt ttatgctcag gattcgagtt
     8821 ggggatcata atcgacaccc aatcgccctg cttcactttc agcgcatccg ccacgccttt
     8881 gccgatgata atttgctgtt cgcccgcttt aaaattgcgc caggcatccc cctgaacaaa
     8941 cgagggtaat gcgctcagac gctgttcctg ttgcgggtta acgcccttca cctggattgc
     9001 gcgcagattc gctccacttt ccaccagccc ggtgaaattg atatacggcg cagcggcggc
     9061 aatacctggc actttttgca cgttatccag tgcttcctgc cagttagtcc acggttgatc
     9121 caccgcctct atttcgccat gcggcaccac cgccagaatg cggttattca gttcgcgttc
     9181 aaagccgttc atcgcgctta agccgacgat caataccgcc acgccaaggg caatgccaat
     9241 ggtagaaatg acggagatca gcgacaccat gccgccgcgt cgccgtccgc ggctaaaacg
     9301 caggccaatc aataacgata aaggcatcgc cattactccg cccccatcag gctcagttcc
     9361 gccgtcagac gaccatcacg catctccagt tggcggctca tacgtttcgc cagttgcagg
     9421 tcgtgagtaa ccaccaggaa ggcggtgccc tgcaagcgat tcaattcccc aagcaactgg
     9481 aagatgctgt ctgcgttacg cgcatcgagg ttaccggtag gttcatccgc cagtaccagc
     9541 cacgggttat tgaccagcgc acgggcaatc gccacacgct ggcgttcgcc gccagaaagt
     9601 tcagatgggc ggtgattcgc acgatgctcc agccccaccg cttttaacat ctcaagtgca
     9661 cggctgttga tttcagcggg ctttttcttg ccaatcagca gcggcatagc cacgttttcc
     9721 agggcagtaa aatccggcag caggtggtga aactgataaa taaagcccag cttctggttg
     9781 cgcagttctg ctttcgccgc cgaagacagt ttgctcatcg gttgaccgtt aaagatcaca
     9841 tcgccggagg ttggtgtatc cagcccgccc agcaggtgca gcaaggtact tttaccggaa
     9901 ccagagctac cgacgatcgc catcatttca ccttcgccca cgctgaaact gacattgtgc
     9961 agtacatcgg tttgcacact gccttcctga tagcgtttgc acaggttgtc gcattgcaac
    10021 aggatcttat tcataacgta aagcctcagc gggttgtgtg gcggcagcgc gccatgaagg
    10081 gtaaagcgta gacagcagcg cgatagccat cgccaccagc gcaataacaa tgacctgtaa
    10141 aggttcgata gccaccggca gcgccgcgcc atcaagcagg acgccgatta tcggcatcag
    10201 attatttaac tggctggcaa gcagtgcgcc aagcgccgct ccgaggatcg caccgataat
    10261 cccggcgctg gccccttgca ccataaagac catcatgatt tgtcgcggag ttaagccttg
    10321 cgtttgcagg atcgctactt cgccctgctt ctccatcacc atcagcccca gcgaggtaat
    10381 aatgttaaac gccgcaacgg cgacaatcag gctcagcagt aagcccatca tatttttttc
    10441 catgcgtacg gcctggaaca gctcgccttt acgatcacgc cagtcctgcc atttgctgcc
    10501 ttcaggcagt ttttgctgac ttaaagagtc aactttcagc ggctcatcca gccacaaacg
    10561 ccagccggta atattgcctg ccggataacg catcagacgc gaggcatcct caatattcac
    10621 cagcatttca tagccatcga cttcactgtt agcggcaaaa gtaccaatca cattgaacag
    10681 gcgctgactt ggaatacgcc ccatcggcgt gaactggctg gcagatggca ccatcacgcg
    10741 gatttgatca ccgcgattaa cgcctagctg tgaggcaagt tgttcgccga ggatgacatt
    10801 atatttcccc ggctcgaggt cagtttgttt cacattgacc agatacggcg ttagtggatc
    10861 tttttgcgcc ggatcgatac ccagcatcac cccgaccgcc acgctgcgcg cgctttgcag
    10921 taccacatca ccggtagtaa taggtgcgac gcgattaacg ccgtccagtt tgactgccgt
    10981 ttccgggagt tgctgcgggt taagagagcc atgctcagaa gagagaattg cctgtggcat
    11041 caggccaagg atgttgtttt gcagctcgcg ctcaaagccg ttcatcactg acaatactgt
    11101 gaccagcgcc atcaccccga gggtaatgcc gatggtagaa agccaggaga cgaaacgacc
    11161 gaagcgatcc gctgcacgcc cacgcatgta acgcaggcca ataaatagag cgacaggttg
    11221 gtacatgaaa tccgtctggt tgctgtagca aagacacgag tatataagcg aaagcaagat
    11281 gggtaaacgt ctgaactgta agtgatcgtc agggctaatc tgaaaagccc ccgtaaatca
    11341 atcggctaaa ttgccatgtt ttatcatcaa tcttgccgcc tgatcttaat ctgaaaaatc
    11401 atccaataca gacagatacc cattacacct cgggtcataa cccgcaggat caaccgcatg
    11461 taaattaaaa aagagcgtgt tgtcctgatg aaacaaaaag agctatggat taaccagatc
    11521 aaagggttat gtatttgtct ggtggtgatt tatcactcgg tcattacctt ttatccgcat
    11581 atgaccactt ttcagcatcc gttatcggaa gtcctgagca aatgctggat ctatttcaat
    11641 ctttaccttg caccctttcg tatgccggtt tttttcttta tatccggcta tttgattcgc
    11701 cgctatatcg acagcgtacc gtggggaaat tgtctcgata aacggatctg gaacatcttc
    11761 tgggtgctgg cgctttgggg cgtggtgcag tggctggcgt taagtgcgct gaaccagtgg
    11821 ctggcacctg agcgcgattt aagcaatgcc tccaatgccg cgtatgccga ttccaccggt
    11881 gagttcctgc acgggatgat caccgccagc accagcttgt ggtatctgta tgcgttaatt
    11941 gtctatttcg tggtatgtaa aatttttagc cgtctggcgc tgccactatt cgccttgttt
    12001 gtactgctga gtgtggcggt taatttcgtt cccacgccgt ggtggggaat gaacagtgtg
    12061 atccgcaatt tgccttatta cagccttggt gcatggtttg gcgcaacaat aatgacctgt
    12121 gttaaagagg tgccgttgcg ccgccatctg ctgatggctt ctttgctggc tgctctggcg
    12181 gtcggtgcct ggttgtttac tatctcgctg ctgttgtcgc tggtgtcgat tgtggtgatc
    12241 atgaagttgt tttatcagta tgaacaacgt tttggcatgc gttccaccag tctgctgaat
    12301 gtgattggct ccaacactat tgctatctac actacccata gaattctggt agagatattc
    12361 agcttaactc tgcttgcgca aatgaacgca gcacgctggt cgccgcaagt cgaactgaca
    12421 ctcctgctgg tttacccctt tgttagtttg ttcatctgta ctgttgcggg cttgctggta
    12481 agaaaacttt cacagcgagc attcagcgat ctgttgttct ccccgccttc actgcccgtt
    12541 gccgtcagtt actcccgcta agccatcgcc ccagccgggg cgattcaatt tgctaaacca
    12601 tgtcgattac ggataatgaa cagcattgct tatcagagat atgcccccat atgttgaggc
    12661 atatcctaac gagaatctga caaccgttat gcctgaacaa tatcgttata cgctgcccgt
    12721 caaagcgggt gagcagcgtc tgctgggcga gttaaccggc gctgcctgcg cgacgctggt
    12781 agcggaaatt gccgaacgtc acgccggacc ggtggtgctt attgcgccag atatgcaaaa
    12841 tgccctgcgt ttgcatgatg aaattagcca gtttaccgac cagatggtga tgaatctggc
    12901 ggactgggaa accctccctt acgacagttt ttcgcctcat caggacatta tctcctcgcg
    12961 cctttccacc ctttaccagc taccgacaat gcagcgtggc gtactgattg ttccggtgaa
    13021 tacacttatg cagcgcgttt gcccgcacag ttttctccac ggtcatgcgc tggtgatgga
    13081 aaaaggtcag cgcctgtcac gagatgcatt acgaacccaa ctggatagcg ccggttatcg
    13141 ccatgttgac caggtgatgg agcacggcga atacgccacg cgcggcgcgt tgctggatct
    13201 cttcccgatg gggagtgagt tgccttatcg tcttgatttc tttgatgatg aaatcgacag
    13261 tctgcgggtg tttgacgtcg acagtcagcg cacgctggag gaagtagaag cgatcaacct
    13321 gctgcctgcg cacgaatttc cgaccgataa agcggcgatt gaactgttcc gcagccagtg
    13381 gcgcgatacc ttcgaagtga agcgcgatcc ggaacatatt taccagcaag tgagtaaagg
    13441 cacattacct gccgggatcg agtactggca gccattgttc ttcagcgaac cactgccgcc
    13501 gctgttcagt tatttccctg ccaatacctt gctggtgaat actggcgatc tggaaaacag
    13561 tgccgaacgt ttccaggctg acacgctggc gcgttttgag aatcgcggcg tcgatccgat
    13621 gcgcccactg ttgccaccac aatcgctctg gctgcgggtg gacgagctct tctcagagct
    13681 gaaaaactgg ccccgagtgc agctaaaaac tgaacattta ccgacaaaag ccgcgaatgc
    13741 caatttaggt ttccagaaac tgccagacct ggccgttcag gcacaacaaa aagcgccgct
    13801 ggatgcgctg cgtaagttcc tcgagtcttt cgacggtccg gtggtgttct cggtagaaag
    13861 tgaaggccgc cgtgaagcgc tgggtgaact gctcgcgcga attaaaattg ctccgcaacg
    13921 cattatgcgt cttgatgaag ccagcgaccg tgggcgttat ctgatgattg gcgctgcaga
    13981 gcatggtttt gtcgatacgg tacgtaatct ggcgctgatt tgcgaaagcg atctgctcgg
    14041 tgaacgcgtt gcccgtcgtc gtcaggattc tcgccgcacc atcaaccccg atacactgat
    14101 ccgtaacctc gcggaactgc atattggtca gccggtggtc catctggagc acggcgtcgg
    14161 gcgctatgcc ggaatgacca cgctggaagc aggcggcatt accggcgagt atctgatgct
    14221 cacctatgcc aacgacgcca aattgtatgt tccggtgtcg tcgctgcatc tgattagccg
    14281 ttacgcgggt ggtgcggaag aaaatgcccc gctgcataaa cttggcggcg atgcgtggtc
    14341 acgcgcgcgg cagaaagcgg cggaaaaagt gcgtgatgtg gcggcggaat tgctggatat
    14401 ctacgcgcaa cgcgccgcca aagagggctt cgcgtttaaa cacgatcgtg agcagtatca
    14461 gttgttctgc gacagcttcc cgtttgaaac cacgccggat caggcgcagg ccattaatgc
    14521 ggtacttagc gacatgtgtc agccgctggc aatggatcgt ctggtgtgcg gcgatgttgg
    14581 ctttggtaaa acagaagtgg cgatgcgcgc cgctttcctg gcagtagata accacaagca
    14641 ggtggcggtg ctggtgccta ccacccttct cgcgcagcag cattacgaca acttccgcga
    14701 ccgtttcgcc aactggccgg tacgtatcga aatgctctcc cgtttccgca gcgccaaaga
    14761 gcagacgcaa atccttgcgg aagtggcgga agggaaaatc gatattctga tcggtacgca
    14821 caaactgctg caaagtgacg tcaagtttaa agatttaggc ctgctgattg tcgatgaaga
    14881 acaccgcttc ggggtgcgtc ataaagagcg cattaaagcg atgcgcgcga acgtggatat
    14941 tctgacgctt actgcaacgc cgatcccacg tacgctgaat atggcaatga gcggaatgcg
    15001 tgacctatcg attatcgcca cgccgcccgc ccgtcgtctg gcagttaaaa cctttgtccg
    15061 tgagtatgac agcctggtgg tccgcgaggc gatcctgcgt gaaattttgc gcggaggaca
    15121 ggtttattat ctctacaatg atgtggaaaa cattcagaaa gccgccgaac ggctggcaga
    15181 actggtgcca gaagcgcgga tcgccatcgg tcacgggcag atgcgcgagc gcgaactgga
    15241 acgggtgatg aatgatttcc atcatcaacg tttcaacgtg ctggtttgta caaccattat
    15301 cgaaaccggg atcgacatcc cgacagccaa caccattatc attgaacgcg cggatcactt
    15361 cggtctggcg caactgcacc agttacgcgg tcgcgtcgga cgttcgcatc atcaggcata
    15421 tgcatggctg ctgacgccgc atccaaaagc gatgactacc gatgcacaaa aacgtcttga
    15481 agcgattgcc tcgctggaag atctcggggc aggttttgcg ctggcaacgc acgatctgga
    15541 gattcgcggc gcgggtgaac tgcttggcga agaacaaagc ggctcaatgg aaaccatcgg
    15601 tttctcgctg tatatggagt tgctggaaaa cgccgtcgat gcactgaaag ccggacgcga
    15661 gccgtcgctg gaagatctca ccagccagca aacagaagtc gagctgcgga tgccgtcgct
    15721 attgccagat gatttcattc ctgacgtgaa tacgcgtctg tcgttctata aacgtattgc
    15781 cagcgccaaa acggaaaacg aactggaaga gatcaaagtc gagcttatcg atcgcttcgg
    15841 cctgctgccg gatccggcgc gtaccctgct ggatattgcc cgtctacgcc agcaagcgca
    15901 gaaactgggg atcaggaagc tggaaggtaa tgagaaaggc ggcgtgatcg aatttgccga
    15961 gaagaatcac gttaatccgg cctggttgat tggtttgctg caaaaacagc cgcagcatta
    16021 ccgccttgat ggtccgacgc gcctgaaatt tattcaggat ttgagtgagc ggaaaacgcg
    16081 tatcgaatgg gtacgccagt ttatgcgtga actggaagaa aacgcgatcg cttaatggta
    16141 aatcgggggc gtttctgcgc ccccatctgc aacatttaca aattctttgc acttccctgc
    16201 actatccgac actgtcacca tccataattc aggcttatct gtttattaca ataaccttat
    16261 atttattatg gatttttggt gatgatcaaa acgcgttttt ctcgctggct aacgtttttt
    16321 acgttcgccg ctgccgtggc gctggcgcta ccggcaaaag ccaacacctg gccgctgccg
    16381 ccagcgggca gtcgtctggt tggcgaaaac aaatttcatg tggtggaaaa tgacggtggt
    16441 tctctggaag ccatcgccaa aaaatataac gtcggctttc tcgctctatt acaggctaac
    16501 cccggcgttg atccttacgt accgcgcgcg ggcagcgtgt taacgatccc gttgcaaacc
    16561 ctacttccag acgcgccgcg cgaaggcatt gtgatcaaca ttgcggagct tcgtctctat
    16621 tactacccgc cgggtaaaaa ttcggtaacc gtgtatccaa taggtattgg tcagttaggt
    16681 ggtgacacgc tgacaccgac aatggtgacc accgtttctg acaagcgtgc aaacccaacc
    16741 tggacgccaa cggcaaacat tcgcgcgcgg tataaggcac aaggaattga gttgcctgcg
    16801 gtagtgccgg ctggaccgga taatccaatg ggccatcatg cgattcgtct ggcggcctat
    16861 ggcggcgttt atttgcttca cggtacgaac gccgatttcg gcattggcat gcgggtaagt
    16921 tctggctgta ttcgtctgcg ggatgacgat atcaaaacac tctttagcca ggtcacccca
    16981 ggcaccaaag tgaatatcat caacactccg ataaaagtct ctgccgaacc aaacggtgcg
    17041 cgtctggttg aagtacatca gccgctgtca gagaagattg atgacgatcc gcagctgctg
    17101 ccaattacgc tgaatagcgc aatgcaatca tttaaagatg cagcacaaac tgacgctgaa
    17161 gtgatgcaac atgtgatgga tgtccgttcc gggatgccgg tggatgtccg ccgtcatcaa
    17221 gtgagcccac aaacgctgta aatcctggat aaaaaaaatc cccgcgacac ggcatgtcgc
    17281 ggggaaaaaa tcagcagtag caggcattaa tgagggttaa tgcttattta taaattactg
    17341 ctgttccatg gagggtattc ggaccggtta cagaagtaat acggaaagat tttgcgccca
    17401 tctcatccgc tttttgcgcc agctgctctt ccagcgatcc cagatttgtc cccgcgttag
    17461 cactgattgt accgactttt tgttggcctt ctggcgttga ctgaacttcg acagccgcaa
    17521 agctggcaaa tgacatggag cttaaaatcg ccgcagcgat gagggttttt acgtttttca
    17581 taatagtggc cttatgcaga tgaatgacgt cgcaagcaat tacttaacga tcgataacta
    17641 aatgatagat gtgatctgga tcac
//
LOCUS       DAZFDC010000096        17407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFDC010000096 DAZFDC010000000
VERSION     DAZFDC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.096"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..170)
                     /locus_tag="ACHJ48_003374"
     CDS             complement(<1..170)
                     /locus_tag="ACHJ48_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708860.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGALG"
     gene            382..3740
                     /locus_tag="ACHJ48_003375"
                     /pseudo
     CDS             382..3740
                     /locus_tag="ACHJ48_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
     gene            3740..10054
                     /locus_tag="ACHJ48_003376"
     CDS             3740..10054
                     /locus_tag="ACHJ48_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4708861.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTHP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            10278..13136
                     /locus_tag="ACHJ48_003377"
     CDS             10278..13136
                     /locus_tag="ACHJ48_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4708862.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            13139..>17407
                     /locus_tag="ACHJ48_003378"
     CDS             13139..>17407
                     /locus_tag="ACHJ48_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708863.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKHDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEISKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAQKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNELNLFVEVDHHAKFSSNIYSASPSTVGFEHISNLYAPQTIDACFE
                     HSGSGDIPGLKDEIESEGKLKVVWNTSGHRSRLISIATHELELFARLYDSEGTPAWQA
                     RLPALHAKQLVAVLEKFANQPNRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRENCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPCVTWTELAEDEPKKVTDYYRLAIRAMLAQSGERTLISAIYPPEISHMNAVRSYC
                     YSSQNLLLEHSGMCFSLPFDFICKSTGKANLHQMLDGFSYVLFNP"
BASE COUNT         4632 a         3883 c         4410 g         4482 t
ORIGIN      
        1 cctaatgccc ccattaaggt tggagcatat tctctaaact tactatgtat attttttttg
       61 tcacaccacc acgctgataa aaatacgaat ataacagccc agatgaaaat attggtgatc
      121 agtgtagagt ctatcgattg aaacaactgc tcaaaaagct gcgctaacat tttgatacct
      181 gtgtaaataa tggatagttg taatcgttta aaaaataaac aaaacacaca taatttatat
      241 ggttaagttc ggtgtagcgc tcatgattgt aacaagtttt ttcgagatat aaatgatttg
      301 tctcattttt agcgagttca tcgggttgag cttttttatg atgatagtaa cctgttacga
      361 attgtatcaa aggaaggcag gttgcttgtg aaaactccag ttaacccact tttgcaatgg
      421 cttaacatgt tttttagtcg ccgttcatta tccggagctg atggacgggc gttatatgct
      481 taccgttgta ctgatacaga gtacgaaagt ttggcagaac tactgcgtac atatgccccc
      541 gtagttatcc aagaacgata ttcatttcct atagcgatgt tctatttagc atatatgccg
      601 cagagtttat ccgccggact catacggtcg gacaccctaa atgggacaca attttagatt
      661 ctattaactg gaaagttccg tatgttcatc gacagaagct ggttaatgat ggcattcgct
      721 actggaaaag aaagataaga aacctgggac aagcttcggg ttatctgcat acactggctt
      781 gtgaaggtgg tttgcctatc cgcatgattg aaaatgagag cggttatttg attacctatt
      841 tcagaaggat atatcaggcg cttcgtgggc aatcatctca atatcctgcc gctaaaattg
      901 cacaagaatt aggcgatacg attcctgtca caatgcaaaa cgaactggtc tatgaaatag
      961 ccggtgaatt ctgcgagact ctctgccgat tacttagtga gcatcctcct catagcagtg
     1021 atcccgtttc tgcattacgt aagctctctc cagactggca tcttcaactt ccactcgttc
     1081 tccccgaagc gaatgctgca gagattgtaa ggcgattact ttctcaatct tctgaaatac
     1141 gcagtgcaag tagtcttcag gttgaaagaa tctgggtcga tgttgatgac agttggtatt
     1201 gcgatgcccg gttccgattc ccggccacaa tgcgtacaga acagttaacc tctttgtttg
     1261 aatgccatat tcagccggag cagacccgac tcatcatttc aggaaaatgg aaaaatggcg
     1321 gggcaaggtt ggcaatgcta agccgttatg agcagcaaga ttggcgggtc gagttattac
     1381 ctattgcgat gcaaaaactc tctggtgcag atgcaatggc cgaaatctca ttgtcgcttc
     1441 atgaaggtcc aattctgtta ggtcacacaa ttccaaaagg cggttatgaa cttactgaag
     1501 aacttccctg ggtttttgaa gcgatgaatg agagtgaatc gcagctaaaa cttgttggta
     1561 tggggtctgt gagttccagg ctaaatgctc tgtttatttc gctaccgaaa aatagccatt
     1621 tagatattag tggagaaggt gagtttgata tcccaaggtt gctgaaaaac agtgaacgca
     1681 gcttaactaa aataagtgga gtatttagcg ttgttttaca tgatggtgct gtttgtacaa
     1741 ttcgcaccca gcaactttat gattctgcga ttgaatatta tattaaatcg acagaagttg
     1801 aactggttaa atcggattat cctgtccatc gagcctggcc gaagatcggt tggaaaaagg
     1861 atctgcaata tggcattgta ccggagaaag aacttttttg gcgttccatt cgttcaggta
     1921 ataatgcctg gtattctgtc gcatcagaaa tgccaaaagg acagatagaa gtccggcgaa
     1981 tagttaatga tgaggtttta tttagcggta aagttgtagt tttaccagca gacttcgata
     2041 ttaatattat acctgagagt gctcagcaag gcattattat gctttctggt ataacggata
     2101 ctagaattga taaatattca aacaatgaaa aagtaacact taagtccgat tattcacaaa
     2161 acgagtgtgc tatttattat aattcatcgc tgatgctgga aaataccgtt gatttacggg
     2221 tctcctggaa agatggttct aatcttaaac tattattacc taagccggtt agcggtggcc
     2281 gatttgtaac taatgatggt tctgttcatt ttgatggtgt ggcatctata gcacatttgc
     2341 atggaataga tgctgagtta ttaaccatat catgtgctgg aagaggatat cttaatatcg
     2401 agttattgga tgaaaatcca gtagctgaaa aattccgcta tttacatgcc gaccttcctc
     2461 ttttatcagg acgcaatgac aaattacaac aaatttcact ttatgagaac tataatttgc
     2521 taaatgctat gctggcatgt gcatggaaca gcaatagtac attatgtgtt gatttttact
     2581 ctgacagatt tggaaaggat aaagcaacac tcaatattaa acgctacgat ggtagtttta
     2641 ttgaacacga tcaagggtta ctggttgata taaaaaattc tgttgttttt cctgcaaata
     2701 gaatagacga actggttgtt gatgctattt ctcttaagaa tcctggctta cacatatcat
     2761 tgttaaaaaa agatgagttt gcttatgatc tttcagctct gaatgttcag gatagtccat
     2821 ggttaattgt gggaaaactt gatggtacag ctcgcattgc accagtaatt aaatggatgc
     2881 tacctgtatt gcagacaaat gatttattac taaatgctct atgcgaagca gacccagaac
     2941 agcgcaaaaa aaattttaat gagctaattt ttgaaataga taacaaccca ttgcaaaatt
     3001 attgctgttt attaacagag tatattaaga aatacaaaat gaataatggc ttatccttgc
     3061 tggatctgga cttgttcaga ggtatttcga gtaattaccg cgtggttgtt caattgttaa
     3121 tatcatcatg tctttctggt gatagcgata cgatttatga tatacaggaa gaattaccct
     3181 tttcatgggg atggattccc gtttcaatct ggaaagatgt tttccaaaaa tgttggactt
     3241 atctggagaa acagattaac gataaaacat tagcattaca tatattgcaa ccctttattg
     3301 cttttatgaa ccatcgtgca catatcgatc gtcggctggc tccgattgcg aatatgttac
     3361 ttacatatag tgagagccta ccaaccggtt gtgatgtatt gccaactgtt agtcgtgagc
     3421 agtttaatga agctaaacag atgctattaa ggaaccccga cagctttggg cgtatcagta
     3481 tcttccctaa agaactttgg tctagtgcta ttactccaga gttaaaatct gtttttaata
     3541 agctttggat tgaagataaa tatcactcac ggcttgaaaa acgttttaat ttgatgttag
     3601 tcgcagcgct gttaacccaa aaagataata acctgataca tcaactgtct gcgctttttg
     3661 aatttcacta tcagcaagcc ccacagcaat taggggtaat ctatcaatat tattttgaac
     3721 aagcaggagt atgtcattga tggacgttca gcaactgcat tatgcgccac ttctaaatca
     3781 gcttcatcag cgtgcggtga ggtcggtggt aagtcagttg gcattgcgga gcaagtcgtt
     3841 gactcattat ctggaatcac aatattttca gcctcccggt caggaggggt cactgcttgc
     3901 agacccggta ttcgaaagta cattcggctg gatgcctgcg gatgaaacaa tggaggattt
     3961 gcgggacaag ctgatctcca gcccgctggt tgatgcattg agcaaagcgc cagtgccatt
     4021 tcgtcatcaa cttactgcgt ggcgaacgat tctggaagac aaaaaatcct tgttagtgag
     4081 cagcggtacg ggatccggga aaacagaatg ctttatggta ccggttctgg aagatcttat
     4141 tcgtcagcaa aaatcgaccc gccagtcact aacagggaca caagctattt tcttgtatcc
     4201 gttaaatgca ttaattaaca gccaacagga gcggcttcgc gaatggacgc gcggatttaa
     4261 ggggaaaata cgcttcgcgc tatataatgg cgaaacccgg cataccaaat acgaaattca
     4321 ggaagatcag ttgaaagtac ctgaacaagc tttgagtcgg gaagccattt atgaagcacc
     4381 accttccatc atggtgacga ataccaccat gctggaatat atgctgattc gtcgtaaaga
     4441 tgccccaatc atcgaaaagt ctcaggggat gttaaagtat atcgtcctgg atgaagccca
     4501 tagttatatt ggttctcagg ctgctgagtt agcgctgctt ttacgtcgtg ttatgcaggc
     4561 tttcaatgtt ggacccggca cagataaacc tgtgcagatt atcgcgacct ctgccactat
     4621 tggtgaagac tctcctgaag gtaataagga gcttgctaaa tttgtcgctg atctcgcggg
     4681 cgttacagaa cgtgatgtca aagtggttcg cggttatcga caaataccgc gggtatcgga
     4741 gtcgctgatt cgtcatgaat accccacatc tttaacttct cttaaatccc tgagccctca
     4801 ggatctttac caacagcttt gtcattaccg tgtggcccag caattgcgtc aggcgttaac
     4861 tcatcccgga cggcaggcag tacgtttgag tgagttgctc aatgttgcca gaagaacctg
     4921 gccagatatt aatcaccgtg agctactgca actcctcgat ctaatggcaa gatcgcgaga
     4981 aggtgaactg gcttttacgc cattacgtat gcatgggttt atcagaacac tggcgggtct
     5041 atgggcatgt tcaaacaaac aatgctcaca taaagcacat gaattaaatc agagtgactg
     5101 gccatttggt caggtatggt ttgagcagcg ccaatattgt gactgtggag cgccggtatt
     5161 tgaagtttta cgttgctccg ggtgtggttc agcttatttg tctgccaaag aggagatgcg
     5221 cggtgatgga acgaactggt tggtagcgca accagccgct gccgaagtgg atgagtttgc
     5281 tcttgatgta gatgtttatt ctgaagatga agacaacgac gaattagaca ataactctca
     5341 atttgatcgt ctgattgcga gcgatggtga aaaatatatc atttcactgg aagaaacgac
     5401 ggcggggaaa attgatagtg aagcgcaaaa gcactatgaa ataaacctgt taagaccgga
     5461 atctcgaggt gagggacgca ataactcctt tgcttgtgtt tgctgtggtg acactcaacg
     5521 gaaaaataat cctttattcc gtccgcttcg gctcggcgcg cccttttttt taaatgaaat
     5581 tattcctact ctgctggagt tttctccatt accgcaaaca agagaagagc agttaggacc
     5641 gtttaatggt cgacgcttgc tcacctttac ggattcgcgt cagggtaccg ctcgcatttc
     5701 agcccgctta cagcaggatg ccgataaagc gacgttgcga gctctttgtt accaggagct
     5761 ggcaaatatt acagagcctt cagcaaaact cagcccggca cactgtgcac tgttagagaa
     5821 agttattagc cagttttctg tcctcccttt aaaaaattcc atcgacgcgc tgaaaaaagc
     5881 gatggctgcg aatgaggagc ttagcccgac ggacattcag gcacttaaaa ttattgagcc
     5941 gatggtgagt tccttaagca cggagcatgc tgaagcgatg caaatacttc aggctaactc
     6001 tggcaatcag cagaagaaaa ccatgtcatg gcaaacgctg tctgaacagc tggttggcag
     6061 tgtggacctt gccgatagaa tgagaggcag ttttaaagaa ttgtctgggc ttgatcttac
     6121 taaacaacag tttgcagatt tttgtttata cagcgagttt ggtcgccgcc ctaaaaatgc
     6181 ctggaccctc gaaagtctgg gtctggtagc cattcactat ccattcattg ataaagttac
     6241 aacctgcccg caggactgga agacattatt acccgatccc gataagcaat tagcggaatg
     6301 gcgtaattta ttgaagatta ctcttgattt ctttatccgt gaaaacagtg ccgtttttta
     6361 cgaaaacgag caataccctc gctggatggg ggcacgtttc ccggtaaaaa tgctccaggg
     6421 gccagataaa aaaaacaaag ggcagaaacg tgatcaactt tggccacaaa tacgcgatcg
     6481 ccattataac cgggtcatta agctattaat cgcagtcttt ccggcaatac aacctgagca
     6541 ggctcactgg aaatcgttag ttaaccactt aatgattgag gtctgggatg caattcgacc
     6601 ctgcttacgt cagtttgagt cgggttatca gctggatatt aaacaacaag ctgaatttta
     6661 cagtccaaag caagtgtggc gttgccccta tactcgacgt gcgttagacg tcacgttgct
     6721 gggatactct ccgtatttgc cgggtagcaa agaaattgca cctgaaaaag cagtgttgat
     6781 tgaaatgcct gagcttcctg tacgtcactg gcgtttgtct ggtggtggag aaatcgcccg
     6841 agaagaacgt ctggagtggc tggaaagtaa tgcgctgatt caacatgccc gcgaggaagg
     6901 attatggtct actcgcagcg accgactggc cctgaaagat agctggtatc gtctggagga
     6961 acactctgcg cagagaacgc ctgagcagaa ccagtttaat gaaaaacaat ttaagtcagg
     7021 caaagttaat gtgctgaact gttccacgac catggagatg ggggtggata ttggtggtat
     7081 gagtttggtg gcaatgaaca acgttcctcc agcgccggca aattatttgc aacgtgcagg
     7141 tcgcgcgggg cgtcgtggag agagtgcatc tgctgccatt acgttatgta aaaacacggc
     7201 gcatggaatg gaggtattca aagaccccct ctgggcgttc aacactactg ccagtgcgcc
     7261 acgggttcgt tttgggagca gtagtattgt tcaacgacat gttaatgccc tggttttagg
     7321 tctgttttta cgagctgaag ttcctgatgc gacaaaactg agttgcaaat ggttctttga
     7381 aggtgatgag agccagtgtc ttcgttttct ccattggtta aatcaccagg ccgatcagct
     7441 ggcggataaa cttaaacgcc ttactcaggg aaccgtactg atgtcattga cggcaaccca
     7501 gctcttgacc cgcacacaag caatgatgca gcaggttgat atccgctgga gaagccaact
     7561 tgcgatactg ttggaaaata tcgaagcgtt gaaggcggat aacagcgcgt gggaagaaac
     7621 accagcaggt aaagccatcg cttatcagct tcgtgattat cgcggtgcat atctgttttc
     7681 taaactcatt agtgaagcgt tcctgccggg gcatggtttc ccggttggcg tagtcaattt
     7741 caattatctg acggcagatg agctggaaaa acgtcgcgca ataaaagcca ctcaggctga
     7801 ccctaatgaa gggggagaaa gcttttctcg tcgtattgaa aaattgccca gccgtgattt
     7861 gcctacagca ttacgcgagt atgcgcctgg tgcagatgtg gtattgggcg gaaaagtata
     7921 tcgttcctcg ggcattatgc ttggcaaggt gcttgccagc gggcaggagc tgtcaggcga
     7981 tcatcatatt ccgtggttct ggcattgccg taaatgtggt gcgggggcaa ccagtactac
     8041 ccatcctgtc gaatgtagtc actgcaaagc ggatatacag caacttgatg tgaagcgtta
     8101 tttacagcct gttggttttg cgacagatat tcgttatcag gcacataatg atgtatccat
     8161 gcctgcacaa cttccctgga aagatccacg tgttcttgta ccgtcttcgg tgtgggtttc
     8221 tttacccgat gctgggctag gacgttaccg ttttagccat agtggggaat tgttccactt
     8281 cagtgaaggg gaatttgggc acggctacgc aatttgcctc tcatgtggtc gggcagaatc
     8341 tcaaactcaa ccccagcgta cccctgaaaa tctaaagaac ccagagaggg aaaatacgca
     8401 ttacctgttg cgagggggca gtaacgatcg tcaggggagc aataaattgt gccacggtca
     8461 tgtgcataaa gatctgtggt tgggatatag cagtcgtaca gatatggttg agctgcaact
     8521 caatgatgat aatggcctgt tgatccgtga tgaggttgcc gcgcgttccc ttgcggtcgc
     8581 tttgcgtgaa gggttagcgc ataaattggg tatcgaaaat actgagctgg gcgtgacgac
     8641 gcagcaagcg cgcgatatca atggctatac cggatattcc atcttcattt acgacaataa
     8701 tgcgggtggt gcaggttatg cagtgcaatt gatcgaccat tgggctgatg tctttaatta
     8761 cgccagaaaa ttgctcgatt gcagttgcga taaattctgt caccactgct tgctcagcta
     8821 cgacagtcag cattatgtca acagattaga tcgtcaccat gcattaaccc tgcttaccaa
     8881 tgtacggttg cagcgactta acctcgcgcc agaatatcag tacttcggcg atggtagccg
     8941 ggttgaaact aaccctctga gtttacgtat tgcgcaatgc ctgaacagcg agatttatga
     9001 ctcctgctcg ctggttcttg cggggccgca ggaacagtgg gactttgccc aatggccatt
     9061 atttaaagag ttgctccagt ttgcctcctc cggtgggaat gttgagttgc tggtggccac
     9121 gccgctggca aacttaactg atagctccag acatcagctt tccgcgttgg cggcgatgcc
     9181 gggaggtcgc ttacaagtga aatccattgc cactgcgcag ctcatgcaag gaaaggggcg
     9241 ctggcttgcg caggtgactc gcgaagggca aagccaacag tgggccgcag acgacagcgc
     9301 taccgttgca cctggtgaat tatggggaca aagtgcgtct tcaccagtag tgacacttaa
     9361 aggaacgtcc ggaaaaacct tctcgggtca gaccttaagt gcagaagatt tactgcccgc
     9421 cttacccacc ggggcggtgc gcatcaatct ctgtgaacaa ctggacgggc cgctggaagg
     9481 ttttggttcg cgtttctggt cgttagtcac gcaacagcac gctggctgga agcaggcatt
     9541 tacccgccac aaagagataa cgcatgttga gtacagcgat cgttatctta atagcccatt
     9601 cacggcgcgt ttgttaggtg aaatcttaac cgagctggtt gaacagggaa tggcggaacg
     9661 cgccagcctc actgtttgcg tcaaaaaatt ggattacaac tctcgccaac acgacgctct
     9721 ctataacgcg tggcttaatg aggaagatcg tcagcaggtt gtgaccactt tacttgaaga
     9781 aggatatctg gggcctgcgt ggccgggagc aattagctgg ttaacggggg ataaccagag
     9841 tacggagcat ggacgagaac tgacagtcac cttcagcgat ggcagtcagc attatgtcct
     9901 gctcgacatg gggcttagct actggcgctg tattgaagat accttctttg attttgccct
     9961 gcgagtaccg cagcaagttg aacggctggc aaacacgcga gcgcgagctg ttgccccggg
    10021 taatgacctt aggagttata ttatcgctgg ataaataatt gctcccctgg ggatatcttc
    10081 caggggagtt gacccagctc actttttaat aatttttgtt tgaaaatcat gcgcaattta
    10141 tgcgcattcc ctcccatttt gtcactttgc cgggggcctt catgatatct ttaaggccct
    10201 cttttttatc gcagtgtgcc gcgccacatt tttcaggatg acgttaccgc cactgcccca
    10261 aatagacaga aaaaccgatg aataagcaaa attatgcacc tggtatgcgg gtggttattc
    10321 gtgatgccga atggcgtatt cgccgggcgg atgacagtgg tgatggtggg tatttgctga
    10381 cctgcgatgg tatttcagag cttgtgcgcg gtaaagaagg gttatttctg accaaactgg
    10441 aacaaaaagt agagatcctg gatcccgcaa aaacgcattt ggtggaagat gaatccgcca
    10501 attatcaagc ggcacagttg tatattgaaa gtcagttacg ccaacgcgta ccgacagaca
    10561 gtaaagtcca ttttggtcat ctggcggcga tggattccat gccgtttcag ctcgatccta
    10621 cgcggatggc gctggcacaa ccgcgtcagc ggatactcat tgccgacgct gttggtctgg
    10681 gtaaaacgct ggaagccggt attctggttt cggaactgat ccgtcgtggg cgcggaaaac
    10741 gtattctggt tctggcggtt aaatcgatgc tgacacaatt ccagaaggag ttctggagcc
    10801 gttttgcgat cccattgacc cgcctggatt ctgctggttt acaaaaggtg cgtaaccgca
    10861 tcccaaccaa ccacaacccg ttccactatt ttgacaagac cattatctcc atcgatacgc
    10921 tgaaacaaga cattgaatat cgtcaccatc tggaaaatgc ctggtgggat attattgtca
    10981 ttgatgaggc ccataacgtc gcagaacgtg gaaccagttc gttacgcagt aaactggcaa
    11041 aactgctcgc cgggcgcagc gatacgctga ttatgctttc agcaacaccg catgatggta
    11101 aggctgaaag ttttgccagc ctgatgaaca tgcttgaccc gacggcgatc gccaacccga
    11161 aagagtatga atacgccgat tttgccgata aaaatctggt tgtccgtcgc ttcaaaaaag
    11221 acgtgaaaga tcagatgtcc ggcgagttcc ccgagcgcaa tattgtcaaa ttgactcgcc
    11281 tggcatccgg tgccgaagaa gaagcatacc gtcgtctggt tgagagccag ttccgtgatg
    11341 atgacgatga acaggcccag tctaacaaag gtcgtctgtt caagataacc ctcgaaaaag
    11401 cgctattttc cagcccgatg gcgtgtgcca gcgtcgtggc gaatcgtctg aagcgtcttg
    11461 agagccgtaa agatcataat agccagagcc agatcaacga actggagtca ttgctgttgg
    11521 cgctgaataa cattgatgcc agccagttta gcaaatacca gttgctgctc gacaccatcc
    11581 gaaaagacct cgcctggaaa gccaataaca cggaagatcg cctggtgatc ttcaccgaaa
    11641 gtattaaaac gctggagttc ctcgaacagc aactgcgagc ggatctgaaa ttgaaagatg
    11701 accagatcgc cacgctgcgc ggcgatcagg gcgatacggt attgatggaa accgtagaag
    11761 cctttggtaa aacgcagtcg ccgttgcgtc ttctggtttg ttcggatgtg gcatctgaag
    11821 ggatcaacct gcatcacctt agccacaaaa tgattcactt tgatattccg tggtcgttga
    11881 tggtattcca gcaacgtaac gggcgtattg accgctatgg gcaaaaacat cagccccaaa
    11941 tccgctatct gcttaccgag gccagcgaac cacagatcaa tggcgatatg cgcgtactgg
    12001 aagtgttgat caacaaagac gaacaggcgc agaagaacat tggggacagt tcggagttta
    12061 ccggcaaatt tacccaggaa gaggaagaag agcaggttgc agagttcatg atgcaggatg
    12121 atggtgccag cctgtttgat caactgctga acagcaacgt ctcggaaagc gccgaacacg
    12181 atctgtttgg cgaaatatgc agtgcggttt ccagcgatgc ctcaatggtc accgaaacag
    12241 acaccagctt gtttgccagc gaacaggcgt actgcgaaag agcattgggt tacctgaaag
    12301 ccagtggtca aactatccag tatgaaacct tgcctgataa taccttgtcg ctggtggcac
    12361 cggaggagtt acgccgccgc ttcaaccagc taccgcctga aattgccccg gagaactggc
    12421 agctctattt aagtcaggat aaaaccgtca tcaccgacgc gattgcgcgc gctcgcggtg
    12481 agcaacatgc ctggcccgat gtgcagtatc tctggcaaat caacccggta gtgcagtggc
    12541 tggacgataa aatctcttcg gcttttggtc gtcatcaggc tccggttatc cggctgccat
    12601 acctgcttga acccgatgaa gatcacttca ttctttccgg gttattcccg aaccgtaaat
    12661 cacatccgat ggtgaacccg tggatagtgg tgagctttaa ccgtgaatcg ctaatcggca
    12721 gtcagccttt cgctgagttt ttacaacgtc atccgcagtt gagtaacaag ctgactaaca
    12781 gcggcggtaa agatcgtaat caccaacgcc agcaggattt actggaagcg gctattgctc
    12841 atgcccggga ggtattcatc catgatcgga atgcgtttga aacgcacatt aaccagcaac
    12901 tgaatgagca tctgcaaaag ctggacgttt tgcgtgggcg gcagttgagc caacttgagc
    12961 tggattttgc cgataacaaa cagcaattgt cagtcaagca gagccgtaaa gagcagagac
    13021 aacgcgaaat cgaacacaat tttgacagct atatcgaatg gattgaggac accatgacga
    13081 ctgaaaaaga accctacatt caggtaattg ctgttatcac cggagcggag ggttaatcat
    13141 ggcgctggtc ggtattaata acgaaaacga attttactct aaccactatt tgggtgaggt
    13201 tttcaccagt gatatccgcg atgtgctgga accctggata gcccaggaaa atgcagcgcg
    13261 tgaagcggag cgtgccgctc gtgaacaggg caaagacgtg gagccgggat accgcgctcc
    13321 gtggaaccag tttaacagtc tggcgactga gtttttccgc aaacttgccg agcacgaaaa
    13381 acagcgtcag atcccgcagc gtctggccga tcaacgtaat cgctggcagc cattgttaaa
    13441 ggcgctgggc tacgaaatta cgccacagat ccaaatgctg gatgacgata caccgctgcc
    13501 ggtactggcg cgttacaaca gcactgacgg tagcccgtgg ctgtggattg ttgaagcaca
    13561 cgatcaggaa gaaggaacgc tggatccgct ggcgctctcc ttactgaccg cgcaattccc
    13621 ggcggatacc gacaaacata agcacgacag cctgcgcaaa aaagccaacg gtgaatatcg
    13681 cagctggcag gatttgctct ctacggcggt cttcacccaa aatgaaccgc cgcgttttgt
    13741 gctgctgctc ggtaaccgtc agctattgtt gttggaccgt actaagtggg cgcaaaaccg
    13801 tctgctacgt tttgattttg aagagatttt aagccgtcgt gaaacggata cgctgaaagc
    13861 gactgcggtg ttgttacata aagattctct gctgccgggc agtggggcac cttatcttga
    13921 ctcgctggat gacaattcgc acaaacatgc gtttggtgtt tcggaagatc tgaaatatgc
    13981 cctgcgcgaa agcattgagt tgctgggcaa cgaagcgatg cattatctga tcgaccgtgg
    14041 cctggcaaac tatacaggta atcgtgcggt ggacccggat gaactgagcc gcgaatgtct
    14101 gcgttacatg taccgcctgc tgttcctgtt ctacattgaa gcgcgcccgg agctgggtta
    14161 tgcgccaatg accgccaaaa cctatctgca aggttacagc ctggaaacgt tgcgcgatct
    14221 ggagatgatc ccgctgacca gcgaagaaga tcgcaacggg cgctacttcc acgacagcct
    14281 gaatatgctg tttaaactgg tgcgcgaagg ctacaacggc ggcgtgaaaa tgcagagtga
    14341 cctggagagc ggcgaccgga tcaccatcca tagtcatcag ttcagcgtcc cgcgtctgga
    14401 aagtcatctg tttgatgcca acaacacgcg cattcttaac cgcgtggtat tccgtaacga
    14461 aaccctgcaa cagattatcc aggcgatgtc gttaagccgc ccggccaaag ggcgctttaa
    14521 ccgccgcgga cgtatttctt atcgccagtt gggtatcaac cagttgggtg cggtgtatga
    14581 ggcgctgctc tcctatcgcg gattcttcgc cagcgaagat ctctacgagg tgaagaaagc
    14641 cggggaagag tttaacgagc tggagacggg ttacttcgtc agtaaggatg agattagcaa
    14701 ataccacgaa gacgagaagg tctacgagaa agacggcagt ctgcgcattc accgcaaagg
    14761 cagctttatc taccgtatgg ccgggcgcga ccgtgagaaa tctgcctctt attacacccc
    14821 ggaagtgctg acccgctcac tggttaaata tgccctgaaa gaactgttta aggagcagat
    14881 tgatcccatt agcgatccgc acgccaaagc tgatgccatc ttaaacctca ccgtgtgcga
    14941 accggcgatg ggcagcgcgg cgttccttaa cgaagccatc aaccagctgg cggaagcgta
    15001 tctgttccac aagcagcagg cggaaggtcg ccgtattccg caggatcgtt acacccagga
    15061 gttacagcgg gtgaaaatgt acattgccga caacaacgtt ttcggcgtgg acttaaaccc
    15121 ggtggcggtg gaactggcgg aagtgtcgct gtggctgaac gccattagtg gcgatgcgtt
    15181 tgtaccgtgg tttggttacc agctgcactg cggtaactcg ctggtgggcg cgcgccgtca
    15241 ggtgttcaac aagagcgaac tgacctacaa aaaagccaaa gatccgagct ggcttaacag
    15301 cgagccggtc gaactggcga tgaacacgcc gcgtgaagag acgcagattt tccacttcct
    15361 gctgcccgac ggcggtatgg ctaactacag cgataaaact gttaagcagc gttatccgga
    15421 tgacttcaaa gcgctggaca gctggcgcaa agagtttatt aaaagctttg ccgggcatga
    15481 gattgctgat gtgcagcgta tcagcgaaaa ggtggaagca ctgtggaaca cctatcgcca
    15541 gcaacttaaa gcagaacgtc tgaaaaccgc cgacagctac ccggtgtggc cggcagaaaa
    15601 cagcgagcag acgcgttctt cgctgagcag taaagatgaa acctttagcg gtcgtcttga
    15661 agataacagc gcctaccaga agctgcgttg ggtaatggac tactggtgcg cgctgtggtt
    15721 ctggccgatc gacaaagccg atgagctacc ggatcgcggc acctggttgt ttgagattga
    15781 aaccctgctt gacgggattg taatcacgga aaaagtcact gaagttgcgg agcacaccac
    15841 cggcgatctg tttgccgaag aaggcctgct gcgagaagag tcttcgctgt tttctgttgc
    15901 tggtcgtctg aaaaccgagg tgttgttccg tcatttgccg cgtctggcga ttgtcgatgc
    15961 cctgagaaag cagcaccgtt tcttccactg ggatctggag ttctgcgacc tgtttgccga
    16021 gcgcggcggt tttgacctga tgctcggaaa cccgccgtgg ctgaaagtgg aatggcagga
    16081 agcgggcgtg ctgggtgatt acgagccgga atttgtgctg cgtaagctga gcgcctcgaa
    16141 gctggcaacg ttgcgtattg atacctttaa ccagatcccg gcgctggaag cggcctggcg
    16201 cagcgagtat gaaggctgtg aagggatgca aaacttcctg aatgcgcagc agaactaccc
    16261 ggtactgcgc ggggtgcaga ccaacttgta taaatgcttt ctgccgcagg cctggcgatt
    16321 aggggcgcag aaaggcgtgg caggtttcct gcacccggaa gggatttatg atgacccgaa
    16381 aggcgggcaa ttacgtgcgg cggtatatcc gaggctgagg gcgcattttc agtttcagaa
    16441 tgagttaaat ttgtttgttg aagttgatca ccatgcgaag tttagcagca atatttattc
    16501 tgctagccct agcacagtgg gatttgaaca tatatctaat ttgtatgctc cgcaaactat
    16561 tgatgcatgt tttgaacatt ctggcagtgg ggacattccc ggtctcaaag acgagattga
    16621 gagcgaggga aaattaaaag ttgtatggaa cacatctggc caccgttctc gattaataag
    16681 tatcgccact catgagctag aattatttgc tcgtctatat gacagcgaag gaacgccagc
    16741 ctggcaggca cgtttgccag ccttacatgc taaacaactt gttgctgtac tggaaaagtt
    16801 tgctaatcag ccgaatagat taggtgattt gcaggggcag tatttttcaa cggttatgtt
    16861 cgatgaaaca tatgctcaga gggatgggac aattttacgg cagactcaat tccctcaaga
    16921 ttcatcacaa tgggtactgt ctggccctca tttctttgtt gggacgccgt tctacaagac
    16981 tccgcgcgaa aactgtacgc ttaacagcga ttatgactgc ctggacttgc taactctgcc
    17041 tgacgactat ctgccgcgca ctaactacat tccggcatgt gatgcacagg agtatgcaaa
    17101 acgtactcca tgcgttacat ggactgaact ggctgaagat gaaccgaaga aggtaacaga
    17161 ttattatcgc ttagctatca gagccatgtt ggctcaatcg ggggaacgta cactaattag
    17221 tgctatttat ccgccagaaa taagtcacat gaacgcagta cgttcttact gctatagctc
    17281 acagaatctg ttactcgaac attcaggtat gtgtttttct ttaccttttg attttatttg
    17341 taaatctact ggcaaggcaa acttacatca gatgcttgat ggtttctcat acgtattatt
    17401 caatccg
//
LOCUS       DAZFDC010000097        17390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFDC010000097 DAZFDC010000000
VERSION     DAZFDC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.097"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(53..1081)
                     /gene="epmB"
                     /locus_tag="ACHJ48_003379"
     CDS             complement(53..1081)
                     /gene="epmB"
                     /locus_tag="ACHJ48_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO4708864.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1123..1689
                     /gene="efp"
                     /locus_tag="ACHJ48_003380"
     CDS             1123..1689
                     /gene="efp"
                     /locus_tag="ACHJ48_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO4708865.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1741..1866
                     /gene="ecnA"
                     /locus_tag="ACHJ48_003381"
     CDS             1741..1866
                     /gene="ecnA"
                     /locus_tag="ACHJ48_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4708866.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            1977..2123
                     /gene="ecnB"
                     /locus_tag="ACHJ48_003382"
     CDS             1977..2123
                     /gene="ecnB"
                     /locus_tag="ACHJ48_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4708867.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2299..2616
                     /gene="sugE"
                     /locus_tag="ACHJ48_003383"
     CDS             2299..2616
                     /gene="sugE"
                     /locus_tag="ACHJ48_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4708868.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2613..3146)
                     /gene="blc"
                     /locus_tag="ACHJ48_003384"
     CDS             complement(2613..3146)
                     /gene="blc"
                     /locus_tag="ACHJ48_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4708869.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3234..4367)
                     /locus_tag="ACHJ48_003385"
     CDS             complement(3234..4367)
                     /locus_tag="ACHJ48_003385"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4708870.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4430..4789)
                     /gene="frdD"
                     /locus_tag="ACHJ48_003386"
     CDS             complement(4430..4789)
                     /gene="frdD"
                     /locus_tag="ACHJ48_003386"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO4708871.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4800..5195)
                     /gene="frdC"
                     /locus_tag="ACHJ48_003387"
     CDS             complement(4800..5195)
                     /gene="frdC"
                     /locus_tag="ACHJ48_003387"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO4708872.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5206..5940)
                     /gene="frdB"
                     /locus_tag="ACHJ48_003388"
     CDS             complement(5206..5940)
                     /gene="frdB"
                     /locus_tag="ACHJ48_003388"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO4708873.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(5933..7741)
                     /gene="frdA"
                     /locus_tag="ACHJ48_003389"
     CDS             complement(5933..7741)
                     /gene="frdA"
                     /locus_tag="ACHJ48_003389"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO4708874.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTKRDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8066..9043
                     /gene="epmA"
                     /locus_tag="ACHJ48_003390"
     CDS             8066..9043
                     /gene="epmA"
                     /locus_tag="ACHJ48_003390"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO4708875.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9262..10764
                     /gene="yjeM"
                     /locus_tag="ACHJ48_003391"
     CDS             9262..10764
                     /gene="yjeM"
                     /locus_tag="ACHJ48_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO4708876.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(10915..14238)
                     /gene="mscM"
                     /locus_tag="ACHJ48_003392"
     CDS             complement(10915..14238)
                     /gene="mscM"
                     /locus_tag="ACHJ48_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO4708877.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14260..15228)
                     /gene="asd"
                     /locus_tag="ACHJ48_003393"
     CDS             complement(14260..15228)
                     /gene="asd"
                     /locus_tag="ACHJ48_003393"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO4708878.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15325..16377)
                     /gene="rsgA"
                     /locus_tag="ACHJ48_003394"
     CDS             complement(15325..16377)
                     /gene="rsgA"
                     /locus_tag="ACHJ48_003394"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO4708879.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16472..17017
                     /gene="orn"
                     /locus_tag="ACHJ48_003395"
     CDS             16472..17017
                     /gene="orn"
                     /locus_tag="ACHJ48_003395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO4708880.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDGWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17228..17303
                     /locus_tag="ACHJ48_003396"
     tRNA            17228..17303
                     /locus_tag="ACHJ48_003396"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17261..17263,aa:Gly,seq:gcc)
BASE COUNT         3999 a         4507 c         4583 g         4301 t
ORIGIN      
        1 aataattaaa ggttatgtaa cgggcaaaga ttaaacttcc ctgtttaatc agttactgct
       61 ggcgtagctg gagatccagc ggcgttttgc tgggttcgcc gccaatttct cgcgccagtt
      121 tcggcaccag atatcccgac accagtgtca gcaactcacg cataatctgc cgtgcttcgt
      181 catcactcac cataaaatgc gccgcgccct gtactttatc gagcacatgc aggtaatacg
      241 gcatcacgcc tgcatcgaac aacgcattac tcaggtttgc cagcgtttgt gcgttatcgt
      301 tcacaccacg taacagaacg ctctggttca gcaaagtaac gcccgcccgg cgcaacttag
      361 ccatcgcctg acggaatgtt tcatctatct cattggcatg gttgatgtga ttcaccagca
      421 agatttgcag cgtagaatgt gaaaagcgtt caaccagcgc atctgtgatg cgtgccggga
      481 tcacaatcgg cagacgactg tgaatccgca gacgtttgat atgcgggatg gcttccagtt
      541 gtgtgagcaa ccagtccagc tcgtgatctt tcgccatcag cggatcgccg ccggagaaaa
      601 tcatctcgtc cagttccgga tgcgcagcaa catactcaag tgcagtttgc cagttacgct
      661 tgttgccctg attttcggca taggggaagt ggcgacggaa gcaatagcgg caatttaccg
      721 cgcagccgcc tttgaccagc aaaagcgccc ggttgtggta tttatgcaac aaaccaggca
      781 ctacgctgtg ctgttcttcc agcgggtcgg tggagaatcc gggcgcgacg acaaactcat
      841 cttgcgaggt aagtacctga cgcaaaagcg gatcgtccgg attgcctttc tccatgcgat
      901 cgataaatga gcggggcaca cgcagggcaa acagcttttt ggcgttgcgt ccggccaaca
      961 gtttttcgtc cgcgtctata ttcaaaagac gcagaagttc atcaggatcg gtcacaacat
     1021 cggcaagttg cgttaaccaa tcttctctgg atggggtatt tagggttaca atatgcgcca
     1081 ttttgtggct tagctaccaa ttaacaaatt tcagagggcc ttatggcaac gtactatagc
     1141 aacgattttc gtgctggtct taaaatcatg ttagacggcg aaccttacgc ggttgaagcg
     1201 agtgaattcg taaaaccggg taaaggccag gcatttgctc gcgttaaact gcgtcgtctg
     1261 ctgaccggta ctcgcgtaga aaaaaccttc aaatctactg attccgctga aggcgctgat
     1321 gttgtcgata tgaacctgac ttacctgtac aacgacggtg agttctggca cttcatgaac
     1381 aacgaaactt tcgagcagct gtctgctgac gcaaaagcaa ttggcgacaa cgctaaatgg
     1441 ctgctggatc aggcagagtg tatcgtaact ctgtggaatg gtcagccgat ctccgttact
     1501 ccgccgaact tcgttgaact ggaaatcgtt gataccgatc cgggcctgaa aggtgatacc
     1561 gcaggtactg gcggcaaacc ggctaccctg tctactggcg ctgtggttaa agttccgctg
     1621 ttcgtacaaa tcggcgaagt catcaaagtg gatacccgct ctggtgaata cgtctctcgc
     1681 gtgaagtaat gcggttgtgg tgcggcctgc aggctgcacc atcacttatt caggtcagag
     1741 atgatgaaac gccttatcgt tcttgttttg cttgccagca cgctgctcac gggctgtaac
     1801 accgctcgcg gtttcggcga agacatcaaa catctcggca actccatctc ccgcgctgcc
     1861 agctaatttt tcttctcttc cgaaaaatca tcagatttcc atcatttttg gcgatgttgt
     1921 ctattattaa tttgctatag gcaaacataa ataacattac ctaaaaggaa gacgttatgg
     1981 tgaagaagac aattgcagcg atcttttctg ttctggtgct ttcaacagta ttaactgcct
     2041 gcaacaccac gcgtggcgtt ggtgaagaca tttctgatgg cggtaacgcg atttctggtg
     2101 cagcaacgaa agcgcagcaa taagcaataa cggtacgaca gctgtgtcgt gccgtttgtt
     2161 ttttctgcga tagtcacaaa ggtaatagtt gaaattcccc cgccacctgg caaaatatcc
     2221 gttcaaccat cagctttgca ggacgacctg caaacgcgtc ttttcaccgg ggacggcccc
     2281 aattctccgg agcctgatat gtcctggatt atcttagtta ttgctggtct gctggaagtg
     2341 gtatgggccg taggcctgaa atatacccac ggctttagtc gtttgacgcc gagtgttatt
     2401 accgtgacgg cgatgattgt cagtatggcg ctacttgcct gggcgatgaa atcgttacca
     2461 gtagggacgg cttatgccgt gtggacgggt attggcgcag tcggcgcggc catcaccggc
     2521 attgtgctgc tcggtgagtc cgctaacccg atgcgtctgg cgagtctggc gttaatcgta
     2581 ttggggatta ttggtctgaa actcagcact cactaactac cgggctgctg tacccaaata
     2641 aacttactga catcaaaccc ttcccgggtc gcgactgcca gcatctcctg tttcacttcg
     2701 tcagaaatgg ttggcgtgcg ggagagtatc cacaggtagt cgcggtccgg tccgcaaacc
     2761 agcgcatggc ggtattcccg atcgagtgca ataacgttat aaccgccata gaaaggacca
     2821 aagaatgaca ctttcagcgc agcgcgagtt ggtgcgccgg taaagtacgc tttcccttca
     2881 ctctgctgcc acattcctct gtcagggtta tagcctttat taatgacatt caggccgccg
     2941 tcatcacgca ggctgtatgt tgcggtgact ttttccagtc cacgttcaaa gcggtgatca
     3001 aaacgggcaa tctcatacca ggtaccgaga tagcgtttag cgtcgaaatt attcactacg
     3061 gtcacgccac gcggcggcgt aggagaactg caggcaacga ccagaaatgc agctgtcgct
     3121 gcggcaacga gagggagcag gcgcataaat gtttccttac tggttttttc taagtgtaga
     3181 tgacagcaag gaaaagcgca gaaaaggtcc gaaaattcgg acccgatgaa attttactgt
     3241 agagcgttga gaatctgcca ggcggcggcg actctcgctg gattgggata gtttttgtta
     3301 gccagcatca cgatacccag ctctttttct ggaataaacg cgacatagct accaaatccg
     3361 ccggtcgccc ccgttttatg cacccatgat gctcgtactg caggagttgg aggcgtaatc
     3421 gcttttacgg ggcgtgctgc cagtgcaatt ttattgtcac tgccgttaac gatgatgtca
     3481 ggatttaccg gccagtccag catttcccag cccagaccct gatacatatc gccggtttgc
     3541 cagtagcgag attgtgccag ttgtatccct tgctgaagtg ttttgtcgtt gatatcacgg
     3601 ggattcatat tgcttcgtac ccagcaggcc atatcttcaa tggtcgactt cacaccataa
     3661 gtttcagcat ctaacgcccc tggcgaaaca tgaactgcct tgccttcgcg atatccccag
     3721 gcgtaattct tttcttctgc gggcggtaca ttaatccacg tatggttgag tttgagtggc
     3781 tggaagacac gagtttgcat cgcctgctca aaactcaaac cagacggctt cacagccagt
     3841 gcgccgaaca aaccgatact ggagttggca tacagacgtt gtgttcctgg agcccatgca
     3901 ggctgccagt tttgatagaa gcgcagcaag tcgcttgagg atttcacttc atccggcacc
     3961 tgcaatggca ggccgccagc agtgtaggtt gcgagatgta atagtgtgat cccattccac
     4021 tgtttagcgg taagttcagg ccagtatttt gttgcgggat cgcttaactt gatttcccct
     4081 cgagcaatag cgtcgccacc aagcacgccc gtaaatgttt tgctgaccga acctaactca
     4141 aacaacgttt gctgtgtgac gggctgcttt ttggcgatgt ccgcataccc ccaggtaaag
     4201 taataaggtt taccctgata aattaccgcc accgccatac cggggatctt ttgttgctct
     4261 ataagcgggg taattgtgcg atgcacaata tcgttgattt gttgaggggc agcaaatgtg
     4321 gagcaagagg cggtaattaa taaggcgcag agcgtcgttt tgaacatagg gtctggtttc
     4381 catacaaaac ggcccgccat aggcgggccg gatttacatt ggcgatgcgt tagattgtaa
     4441 cgacaccaat cagcgtgaca actgtcagga tagcagccag accgtagaaa acccatttgc
     4501 ccgcaggtac gtggattttc agatcgtgca tcgcgtggtg catacggtgt aaaccacacc
     4561 acagcggcag aacgatcatc aggaacagga atacgcgacc aatgaagctc tgcgcgaacg
     4621 ccagaacgcg ctcgtagctc agtgcatcac ccggaaacag ccccagcggc agcagaatac
     4681 ccaccagcag gatcatcacc ggcgcaataa ttgcgctcca cataccaccg gccccgaaga
     4741 ggccccagaa taccggttcg tcagaacgct ttggatttgg attaatcatc tcaggctcct
     4801 taccagtaca gggcaacaaa caggattacg atggtggcaa ccacagttac cgcccagaga
     4861 cttttgataa ttggctctgg tcccattttt tcgtctttta caatgatatt ggccgctttc
     4921 ggtgccagtt caaaccaggt tttggtgtgc agcagagctg ccgccagagt gatcaggtta
     4981 atgatcacaa taaccgggtt ttgtaaaaag tcgacgaatc ccgcccaggc ttccgggcca
     5041 tttttcaggg caaacagccc gaaaatcagt tcaatgctga accacacagc cggaaccgcc
     5101 gtgccttcgc gcagcatgta aaagcgataa aacggcaatt ttttccacca ggtggacgtc
     5161 attggccgta catacggttt acgtttagtc gtcatgttgc actccttagc gtggtttcag
     5221 ggtcgcgata agaaagtctt tcgaactttc tactttgccc tgttgaatgg ccgcagccgg
     5281 atcgacgtgt ttcgggcaga cttcggagca gtagcccacg aaagtacagc tccatacgcc
     5341 gttctggctg ttcaactgcg ccatacgctc cttcttaccg tggtcgcggc tatcttcgtt
     5401 ataacgatgc gccagcgtaa tggcagccgg accgatgaac tctgggttca ggccaaactg
     5461 cgggcacgcg gcgtagcaca gaccacagtt gatgcaaccg gagaactggt gatacttcgc
     5521 catctgcgcc ggggtctgga tgttagtacc ctgatccgcg gtgcgggagt tgccgatgat
     5581 gtacggtttg atcgcttcca gactttcgat gaagtgggtc atatcgacca ccagatcgcg
     5641 ttcaatcggg aagttagcta acgcttcaac cttcataccg tcggtgtaat cacgcaggaa
     5701 ggttttacat gccagttttg gcacgttgtt aaccatcatg ccgcaggaac cacaaatcgc
     5761 catacggcag gaccagcggt agctcaggtc cggtgccagg ttgtctttga tgtagcccag
     5821 cgcatccagt aatgaggtag ttgcgtcata aggcacttca tagaatgcgc tatgcggtgc
     5881 ggtatcgact tccgggttat agcgcaccac ctcaattttc aggtttttca tctcagccat
     5941 tcgccttctc cttcttattg gctgcttccg ccttatcggc tgcatccgct tcgccaccgt
     6001 aaacgcgttt agctggcggt agcgtagtaa tcttcacgtc gctgtactcc aggcgagtcg
     6061 tgccatcagc atcgcggaag gcgagggtgt gtttgaggaa gttgacgtcg tcacgcttgg
     6121 tgcaaccttc gtccagacgc tggtgtgcgc cgcgggactc tttacgtgcc attgcggagt
     6181 gcgccataca ttcagcaacg ttcagaccgt ggcccagttc aatggtgtag agcaggtcgg
     6241 tgttgaacac gctggaagtg tcggtgatgc gcacgcgctt gaagcgttcc tgcagctctg
     6301 ccagcttgtc gatggttttc tgcatcagtt ccggcgtacg gtagataccg caaccttctt
     6361 ccatagccag gcccatttcg tcgcggatct tcgcccagtt ttcgccgcca tcctggttaa
     6421 ccagatcttt cagacgttgt tcaacgccag ctgcctgcgc ttcaattgcc gcttcgttgc
     6481 cattaccggc agttgctgca cgctctgtcg cttgttcacc ggccagacgg ccgaagacca
     6541 ccagttccgc cagggagttg gagcccagac ggtttgcacc gtgcagacca acagaggaac
     6601 attcacccac ggcgaacaga cctttaatgc gggtttcaca gttctgatcg gtttcgatac
     6661 cgcccatggt gtagtgtgcg gtcggacgta ccggaatcgg ttctttaacc ggatcgacgc
     6721 caacgtacgc tttcgccagt tcgcagatga acggcagacg ttcatgcagt tttttctcgc
     6781 cgaggtgacg caggtcgaga taaaccacat caccacgcgg cgtggagatg gtgttgcctt
     6841 tacgccattc gtgccagaag gcctgagaaa ctttgtcgcg tggacccagt tccatatatt
     6901 tgtttttcgg ctcgcccagc ggagtttccg ggcccatgcc gtaatcttgc agataacggt
     6961 agccattttt gttgaccaga ataccgcctt caccgcggca accttcggtc atcaggatac
     7021 cggaacctgg cagaccggtt ggatgatact gaacgaattc catgtcacgc agcggaacgc
     7081 cgtggcttag cgccataccc ataccgtcac cggtaacgat gccgccgttg gtgttgtaac
     7141 gataaacgcg acccgcaccg ccagtagcca taacgaccgc gttagcacgg atctgcacca
     7201 gcgtgccttc catcatgttc attgctacca ggccgcgaac atgaccatca tcaaccagaa
     7261 tatccagcac gaaatgttcg tcaaaacgct ggatctgcgg gaattgcaga gaggtctgga
     7321 acagcgtgtg cagcatatgg aagccggtct tatcggcggc gaaccaggta cgctcgattt
     7381 tcatgccgcc gaagcgacgt acgttgacgc taccatccgg gcgacggctc catgggcacc
     7441 cccacagttc cagttgggtc atttcggttg ggcagtggtg gacgaaataa tccacgacat
     7501 cctgctcaca caaccagtcg ccacccgcta ctgtatcgtg aaagtgatat tcgaagctgt
     7561 catgatcctg cgcgacagcg gcggagcccc cttctgcagc aacggtatgg ctacgcatcg
     7621 ggtatacttt tgagattagt gcgatttttg catttggatt tgcctgcgcg gcagcaattg
     7681 cagcacgtaa tcccgcgcca ccggcgccta caatggcaag atcggcttga aaggtttgca
     7741 cgacattcct ccagattgtt tttatcccac agccccgtac ttcagggtaa gtgcctgaaa
     7801 gttacggtct gcgaacgcta ttccactgct cctttatagg tacaacagta tagtctgatg
     7861 gataagtctg aaatttgacg agatcgattt ttttagtgcg caagagggct aaattatcac
     7921 tgaagatgat taatttaatt actaaaccat cagatcgtgc tttttttagt cactgcccac
     7981 cgttgtttta ttcctgcgta aaatttgtct cgccgtcgcg ttgcgagtag acttcgtgcc
     8041 cttgtcaaaa actggagatt taactatgag cgaaacggca tcctggcagc cgagcgcatc
     8101 cattcctaac ttattaaaac gcgcggcgat tatggcggag atccgtcgtt tctttgccga
     8161 tcgtggagtg ctggaggtgg agacgccttg tatgagccag gcgacggtaa ccgatattca
     8221 tttggtcccg tttgagacac gtttcgttgg ccccgggcat tcgcagggga tgaatctctg
     8281 gttaatgacc agcccggaat accatatgaa acgcctgctg gttgccggtt gcgggccggt
     8341 attccagctg tgccgcagtt tccgtaatga agagatgggg cgttatcaca accctgagtt
     8401 cactatgctt gagtggtatc gaccgcacta tgatatgtac cggttgatga acgaggtgga
     8461 cgatctctta caacaggtgc tggactgccc ggcagcagaa agcctttctt atcaacaagc
     8521 tttcttgcgt tatctggaaa ttgacccgct ctctgccgac aaaacgcaac tgcgggaagt
     8581 ggcagcgaaa ctggatttga gcaatgttgc tgataccgaa gaagaccgcg acacgttgct
     8641 acaattgctg tttacctttg gcgtagaacc aaatattggt aaagaaaaac cgacctttgt
     8701 gtaccacttt ccagccagcc aggcatcact ggcgcaaatc agtaccgaag atcatcgggt
     8761 cgctgaacgc tttgaggttt attataaagg tattgagctg gcgaatggtt tccatgaatt
     8821 gacggatgcc cgtgagcagc aacaacgctt tgaacaagat aaccgtaagc gcgcggcgcg
     8881 cggtttgccg cagcacccca ttgatcagaa tctgattgaa gccttgaaag tcggtatgcc
     8941 tgactgttcc ggcgtggcat taggtgttga tcgtctggtg atgttggcgc tgggcgcgga
     9001 gacactggct gaagtcatcg cctttagcgt tgaccgggca taattctgaa aaattactga
     9061 attaacagcg aagaatggcg tgataagcgc cattctgtag catatgtttt catttttccg
     9121 ctaaaggcca caatttaaga ctggatattt gatatcatcc aggtatcaat tctgtattgg
     9181 tttttacgct gcccgctcaa tgttgggcag tgcagttcag tgggcgcgtt ttatacacgc
     9241 gctgaaatga aggatggttt catgcctcac acgataaaaa agatgagtct gataggactc
     9301 atattgatga tctttacttc cgtatttgga tttgccaata gcccatcggc ttattactta
     9361 atgggttata gtgcgattcc cttttatata ttttctgcat tgttattctt tattccattc
     9421 gccttaatga tggctgaaat gggagctgct tatcgcaaag aagaaggcgg tatctattcc
     9481 tggatgaata atagtgtcgg accacgtttt gccttcattg gtacgtttat gtggttttcc
     9541 tcttatatca tctggatggt gagcacctcc gcgaaagttt gggtaccgtt ctcaacattc
     9601 ctctatggta gcgacatgac ccagcactgg cgtattgccg gactggagcc tacgcaggtg
     9661 gttggtctgc tggcagtggc atggatgatt ctggtcaccg tcgttgcttc aaaggggatt
     9721 aataaaattg cccgcattac tgcggtgggc ggtattgcag taatgtgtct gaatttagta
     9781 ttgctgttag taagcattac tattttgtta ttaaatggtg ggcatttcgc gcaggatatt
     9841 aatttccttg catcaccgaa cccaggttat cagtccggtc tggcaatgct atcgtttgtg
     9901 gtatttgcta tttttgccta tggcggaatt gaagcggttg gtggtctggt cgataaaacg
     9961 gaaaatccag aaaagaactt tgccaaaggt attgtttttg ccgctattgt tatttcaatc
    10021 ggttattcgc tggcaatatt tttatggggc gtcagcacaa actggcagca ggtattaagt
    10081 aatggttccg ttaacctcgg caatattacc tatgtgctga tgaagagcct cggggtgacg
    10141 ctgggtaacg cactgcattt gtcacctgaa gcgtcattgt cgctgggcgt atggtttgcg
    10201 cgtattaccg gactttcgat gttcctcgct tataccggtg cgttctttac gctttgctat
    10261 tcaccgctga aagccatcat ccaggggacg ccgaaagcgt tgtggccgga accgatgacg
    10321 cgcctgaatg cgatggggat gccgtctatc gctatgtgga tgcagtgcgg gttggttact
    10381 gtcttcatcc tgctggtttc gtttggtggc ggtaccgcat cggcgttctt taacaagctg
    10441 acgctgatgg cgaacgtgtc tatgacgctt ccttacctgt tcctcgcgct ggctttccca
    10501 ttctttaaag cacgtcagga tctcgacaga ccatttgtga ttttcaaaac gcgtatgtcg
    10561 gcaatgattg cgacggtggt tgtcgttctg gtggtgacat ttgcgaacgt cttcaccatt
    10621 attcagcctg tggttgaagc cggagactgg gacagcacat tgtggatgat tggcggccct
    10681 gtcttcttct cgctgttagc gatggcgatt taccagaact attgcagtcg catggcgaat
    10741 aaacctgagt tagctctcga ctgatcatat gaaatcctgc aactcatcgt ctctccggcg
    10801 atgagttgtc aggaagtaat gcgtatggat gatactctcc agggccggtg ataactgaag
    10861 atgatgttaa gctgctaaat gagccacgtt gacatcattt ggcagcttca tgcattacaa
    10921 acttcccgcc tgacgacctt tgcccgcaga cgtcagcgtt ctccccgttt gtttaccgtt
    10981 gaggctttcc agacgcatct ggaagggcgg gaatggcata tcgataccgt gggcatggaa
    11041 gccagccaga atcagctggt ggatctcatg gcgtagcggc atacggtgac ccatctcagc
    11101 ggcgtaaata cgcagctcga aaatctgaat cccctgttgc agatccacca ggaagacttc
    11161 cggtgccggg ttgtcgatca ccagcgagca gcgacgcgct gcggtgagca ggatttccgt
    11221 cacttcttca ctgttggcat cggcaggggc cggaatcgtc aacaccacgc gcgtgaccga
    11281 gtcagagagc gaccagttga taaactgctc ggtaataaac gccttgttcg gcacgattat
    11341 ctctttacgg tcccagtcgc tgatggtggt tgcgcgggta ttaattttcg tcacgctacc
    11401 agtaagatcg cgaattgtca ccgtatcgcc aatgcgaatc ggtttttcga acaggatgat
    11461 caggccagag ataaagttgg cgaaaatttc ctgcaaacca aaaccgagac caacaccgag
    11521 cgcggcgacc agccactgca atttcgacca ctcaatacca atcatcgaga agccgaccag
    11581 cccgccaatc agcatcagca gatatttggt gatggtggtg atggcgtagc cggtacccgg
    11641 cgttaaatcc aggtgctgca aaatcgccag ttccagcagc gcgggcagat tgcgcaccag
    11701 ctgcgtggtg atgataaaca ccagaatggc aatcagcacc gcaccgaggg taatcggctc
    11761 cagactttct acgccctgta ccgtggaggt gacatcccac agcgaaatat tttcgaggaa
    11821 gccgaaagcg gaatggattt ctgaccacag cacaatgacc gaaagcaggg cgatcaacat
    11881 caaaattgag cgcactagcc gcaaggactg cgcactgatg gcatcgagat cgacttcgct
    11941 ttcatcgact tcaattgccc cttccgggct actgtgatga tgtgcttcct cttcgccacg
    12001 cgcacgttgc gctaacatct ctgcccggcg atgcttcgcc cggtcaaacg ccagcctgcg
    12061 tcgttggatc agcatccagc ggcggataac gtgataaacc accagtagca ggaaccagat
    12121 ggcaaccgag gtttcaagcc tcgctaacag cgcctgtgcc gttgccagat aacccaccgc
    12181 cgacgccaga atggcaacca gtggcgcgcc aatcatcatg ttccacaaca tatggttggt
    12241 aatattgtcg ccgctgcctt ctttgttgag atacagcggg atcccggcct ttttcaggct
    12301 gagggtgacc accgccagcg caccgcaaat gaggataaag caaagccgtc ccagcgaacc
    12361 ggaaaattcg cggtcgtcga ggttatcgaa catcatcagc gccataatca gtggcacaat
    12421 aagcccgatg ctcatcaggt agtagcgcat cccacgagaa acacgttcgc gcggccagcc
    12481 aaaatgagcg ataaacaagc cgttcgggcg ggcaaaggta gcgcaaatca tcactaccca
    12541 cagcagcggc acggtggctg tgacgccatc accaatcgcg accgccagcg gataaggcca
    12601 cgcctcgcgc aagccgtaac ccagcgtcat ccacagcacc ggcagcggtg acgcgacgag
    12661 aatcgaccag aaaagagtgc gcaacgtcag ccagaagtga tcctgagtca ctttgccgac
    12721 tttcgccgcc gaacgttcaa gaaaacgggt gaaatagcgg cgcgagtaaa tactgcaacc
    12781 gaccagaatc aacgcgccaa acagcggcaa aatcgtctct ttgctggtca gcatcatcac
    12841 actggctttg cccaactgac tgaaggtgtc cagcgaaatg agacgacgca gatcctgggc
    12901 gatttccagc ggccaggcga tggtcatcgg gcgcacgtca gaggtccaga acagatagcg
    12961 gtgggtcgct tcgttcactt ctttcagcgc atcttccagt tgcccgttgg agactttcag
    13021 cttggtcagt tccagcagta gcgtatcgcc accctgcaac aatgagttca gcaactcacg
    13081 ctgagtgcgt agctgtgctt ccagaatacg gttttgctca gcggtcagcg gctgaccgtc
    13141 ggcctgatga atttgccgta gcagcggctg tttattaagc agatcctcat aacgtaaccg
    13201 ttgcacacgc aactgcgcca tttcggtatc aagctgttgt ggtttcggca tttccggcag
    13261 ccgtgccacc tgcgcccgca acgcttcacc gagcagattg gacgatccca gccattgcga
    13321 ctgttcacgc agcgtattca acgcctgccg gacctgtaac gtctggctgg cagcctgacg
    13381 ctgttgcgag gcaacgagat ccatccgctg cgcctgttga ttcagcgccg ccgatagttc
    13441 gcggttaatt ttgaattgcg cgacgatatc tttcggcaaa tcggcactgt tttctgctag
    13501 taattcggta ctttccagcg cccgctctgc ctcaagctgg cgttggctgt tcaattgatt
    13561 acgcaaggcc tgcaaatacg catccagttg ctggctctct ttttcagcca gctcagagcg
    13621 caagcgcgct aattcctggc ggttattggc agacagctgc gccagctcca gttcatcaac
    13681 gagcgcctta agacgtgcag agtcagactg caacgcgaaa ttttgtgcct gattgagcgg
    13741 agtattgccg gtaagcgtcc ccaggcggcg ctcgatctca tttaactgac ggcgggcgtc
    13801 ggtttgctgt tgcggcagtt gattcagcga atcggcaatc tcgcgggcgc gctcctgctc
    13861 ttgctgggcc tgacggcttt tatccagcaa ctgactgctg acctggagaa tttcctgatt
    13921 cagcgcgtcg gtagacatcc ccggcgacac gctgcgcggc tcgtcacgca tgttgtttaa
    13981 ttgtgcgcgc agagtagcgg agagtttcgg ataattatca atgacttctt gatattgctt
    14041 gatgcgctca agggaacctt ttcgttcctc aagcgcattt aaggcagact ggagtgcctc
    14101 tacgacttct ggctgcgcgg gtttcgccgc ttttgcctgc tccagttcct gggtgatttg
    14161 tttgctatcg ggggccgtcg cggcgtacgc cccccaactg aggcaccagg ccatcagaaa
    14221 agtgataatc aggcgcacgt cagcgtttcc tttgatggat tagacctggt cttttttatc
    14281 gtcaaccaat gggctggcgt cgtgttctgc ttcgatctct tcagcaggaa gcggggcggg
    14341 ttcagcgtct ggcgtaacaa aggtttcggt agatactgcc agcggctggc caattttcgt
    14401 gacagacagg ctttccagtt gctcaaccag attcacttta cccggtgcaa acaggttgat
    14461 aacggtagag ccaagtttaa agcgacccat ttcctggcct ttcagcagcg ccacagaacc
    14521 gtcgttttcc ccggcaggcc aggtccagcg cttgatgatg ccttcgcgcg gcggcgtaat
    14581 ggtgcccgcc cagaccgtct caatgctgcc tacaatcgtc gccccgacca gaatctgcgc
    14641 cattgggcca aattcggtat cgaacaggca gatcacgcgt tcgttacggg caaacagatt
    14701 cggcacgttc tgagccgtga gatggttaac ggagaagaga tcgcccggca cgtagatcat
    14761 ctcacgcaga ataccgttgc acggcatgtg tacgcggtgg tagtcacgcg gggagaggta
    14821 agtggtcaca aacgtaccgt tgcggaacag gtccgccatc agatagttgc ctgccagcag
    14881 ggcttcgagg ctgtagttgt ggcctttggc ttgcaggatt ttatcttctt cgattttacc
    14941 caactggctg ataacgccat cggcaggcat gaccaggaca ttcggatcgg tatcgattgg
    15001 gcgtacttcg tcacgcagcg gacggacaaa gaattcgtta aaggtgcggt agctggcggt
    15061 gtccggcttt tgcgcctctt tcatgtcgac cttgtagtat ttaacgaaca gatcgataac
    15121 cagttttgtc agccatcctg cccgcttgct tgcgccccaa cccgccaggc gagtaagcca
    15181 tagtttcggc agaatgtact gtagcgaaag tttaaatgaa tttaacaagg tagcctccag
    15241 gccattgttt tgtcgttcct gatccggcct aaatgccgga tcctgaaaaa aaggggacga
    15301 ttctaacgac ggttagctta attgtcagtc atccgtatca gaaaagtttt tacgcgtttt
    15361 tacctgcgcc atgctttcca gaatacggtg atagttttcg aaacgggttt ccgcgatttt
    15421 tccttcctca accgcttccc ggatagcgca gcccggatcg gtatcgtgtt tgcaatcgcg
    15481 atatttacac agacctaaat agtcatggaa ttcgacaaag ccctgagtga tttgttccgg
    15541 ctccaggtgc cagaggccga actcacgcac accaggggaa tcaatcacat caccgccgtg
    15601 cgggaagtga tacaggcgtg cggcggtcgt ggtgtgctga ccaagccccg agttgtcaga
    15661 gacatcgttg gtcaggatct ctttttgcag ccccagcaac gcgttcagca ggctggattt
    15721 gccgacgcca gactgcccgg caaaaatgct gatgcgcccg gtcaacgcct cttccagcgg
    15781 ttttagccca tcctgagtat ggctggaaac catcaatacg cgataaccga tattgcggta
    15841 gatatccatc tgttcgttga cgaacgccat gccttcgtcg tccagcaggt ctatcttgtt
    15901 gagcacaata atcggctcaa tctgcaaggt ttcgcaggcc accaggtaac ggtcgataat
    15961 attgagcgac agctccggca aaatggcgga gacaatgaca atctggtcga tgttggcggc
    16021 aataggtttc acgccgtcgt agaaatccgg acgcgtcaac actgaggtgc gctcatgcac
    16081 cgcttccacg atccctttga catttacgcc ttccgccgcc ggtttacccg gacgccagac
    16141 tacgcggtcg ccggtcacca gcgaacggat ggtacggcga atattgcagc ggtgaacgtc
    16201 gccatcggcg gattccacat cagcgtgcat accaaagcgg ctgatgacga taccttcatc
    16261 aggctcgcca aacagattgt cgtcgtagtc gggcttctcc ttagacgttt taagacgacg
    16321 ctggtgattg gcgttcacgc ggcgctgctg gcctttggag agtttatttt tactcaatcg
    16381 tactgactcc tggtcgcccc tgatgggcaa aacatctatg atacacgcaa ttgtggatca
    16441 atatagtcac tgtgaatggg tggaaaatag catgagtgcc aatgaaaaca acctgatttg
    16501 gatcgatctt gagatgaccg gtctggatcc cgagcgcgat cgcattattg agattgccac
    16561 gctggtgacc gatgccaatc tgaatattct ggcagaaggg ccgaccattg cagtacatca
    16621 gtccgatgaa cagctggcgc tgatggatgg ctggaacgtg cgcacccata ccgccagcgg
    16681 gctggtggag cgcgtgaaag cgagcacgat gggcgatcgt gaagctgaac tggcaacgct
    16741 cgaattttta aaacagtggg tgcctgcggg taaatcgccg atttgcggta acagcatcgg
    16801 tcaggatcgt cgcttcctgt ttaaatacat gccggagctg gaagcctact tccactaccg
    16861 ttatctcgat gtcagcaccc tgaaagagct ggcgcgccgc tggaagccgg aaattcttga
    16921 tggttttacc aaacagggga cgcatcaggc gatggatgat atccgtgaat cggtggcgga
    16981 gctggcttac taccgcgagc attttatcaa gctgtaaaat ttttgctggt cacgtaagcg
    17041 ccggaatcca cttgcggcgg cgtgattttg acgctaaatt gaacgtcttg ctgataattt
    17101 cagcacttga gataaaaact caaaaaaaaa tttttagggg ggttgcagag ggaaagattt
    17161 ctcgtataat gcgcctcccg taacgacgca gaaatgcgaa aattacgaaa gcaaaattaa
    17221 gtagtacgcg ggaatagctc agttggtaga gcacgacctt gccaaggtcg gggtcgcgag
    17281 ttcgagtctc gtttcccgct ccaaaatttg aaaagtgctg caaagcacag accacccaag
    17341 cgggaatagc tcagttggta gagcacgacc ttgccaaggt cggggtcgcg
//
LOCUS       DAZFDC010000098        17301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFDC010000098 DAZFDC010000000
VERSION     DAZFDC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.098"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..819
                     /locus_tag="ACHJ48_003397"
     CDS             25..819
                     /locus_tag="ACHJ48_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4708881.1"
                     /translation="MTLPTTIYSFPAYLSRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            complement(890..1339)
                     /gene="rplI"
                     /locus_tag="ACHJ48_003398"
     CDS             complement(890..1339)
                     /gene="rplI"
                     /locus_tag="ACHJ48_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO4708882.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1381..1608)
                     /gene="rpsR"
                     /locus_tag="ACHJ48_003399"
     CDS             complement(1381..1608)
                     /gene="rpsR"
                     /locus_tag="ACHJ48_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO4708883.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1613..1927)
                     /gene="priB"
                     /locus_tag="ACHJ48_003400"
     CDS             complement(1613..1927)
                     /gene="priB"
                     /locus_tag="ACHJ48_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO4708884.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1934..2329)
                     /gene="rpsF"
                     /locus_tag="ACHJ48_003401"
     CDS             complement(1934..2329)
                     /gene="rpsF"
                     /locus_tag="ACHJ48_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO4708885.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2656..2931
                     /gene="yjfY"
                     /locus_tag="ACHJ48_003402"
     CDS             2656..2931
                     /gene="yjfY"
                     /locus_tag="ACHJ48_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO4708886.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3060..3746)
                     /gene="ulaF"
                     /locus_tag="ACHJ48_003403"
     CDS             complement(3060..3746)
                     /gene="ulaF"
                     /locus_tag="ACHJ48_003403"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO4708887.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3746..4600)
                     /gene="ulaE"
                     /locus_tag="ACHJ48_003404"
     CDS             complement(3746..4600)
                     /gene="ulaE"
                     /locus_tag="ACHJ48_003404"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO4708888.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4610..5260)
                     /gene="ulaD"
                     /locus_tag="ACHJ48_003405"
     CDS             complement(4610..5260)
                     /gene="ulaD"
                     /locus_tag="ACHJ48_003405"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO4708889.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5274..5738)
                     /gene="ulaC"
                     /locus_tag="ACHJ48_003406"
     CDS             complement(5274..5738)
                     /gene="ulaC"
                     /locus_tag="ACHJ48_003406"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO4708890.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5748..6053)
                     /gene="ulaB"
                     /locus_tag="ACHJ48_003407"
     CDS             complement(5748..6053)
                     /gene="ulaB"
                     /locus_tag="ACHJ48_003407"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO4708891.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(6069..7466)
                     /gene="ulaA"
                     /locus_tag="ACHJ48_003408"
     CDS             complement(6069..7466)
                     /gene="ulaA"
                     /locus_tag="ACHJ48_003408"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO4708892.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIILGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7821..8885
                     /gene="ulaG"
                     /locus_tag="ACHJ48_003409"
     CDS             7821..8885
                     /gene="ulaG"
                     /locus_tag="ACHJ48_003409"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO4708893.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8993..9748
                     /gene="ulaR"
                     /locus_tag="ACHJ48_003410"
     CDS             8993..9748
                     /gene="ulaR"
                     /locus_tag="ACHJ48_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO4708894.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9745..10494)
                     /gene="yjfP"
                     /locus_tag="ACHJ48_003411"
     CDS             complement(9745..10494)
                     /gene="yjfP"
                     /locus_tag="ACHJ48_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4708895.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETVA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWYGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10676..11005
                     /gene="bsmA"
                     /locus_tag="ACHJ48_003412"
     CDS             10676..11005
                     /gene="bsmA"
                     /locus_tag="ACHJ48_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO4708896.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11154..11429
                     /gene="yjfN"
                     /locus_tag="ACHJ48_003413"
     CDS             11154..11429
                     /gene="yjfN"
                     /locus_tag="ACHJ48_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO4708897.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11546..13171)
                     /locus_tag="ACHJ48_003414"
     CDS             complement(11546..13171)
                     /locus_tag="ACHJ48_003414"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO4708898.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDCAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13255..14418)
                     /locus_tag="ACHJ48_003415"
     CDS             complement(13255..14418)
                     /locus_tag="ACHJ48_003415"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO4708899.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14421..15059)
                     /locus_tag="ACHJ48_003416"
     CDS             complement(14421..15059)
                     /locus_tag="ACHJ48_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO4708900.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15069..15467)
                     /locus_tag="ACHJ48_003417"
     CDS             complement(15069..15467)
                     /locus_tag="ACHJ48_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO4708901.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15485..16144)
                     /locus_tag="ACHJ48_003418"
     CDS             complement(15485..16144)
                     /locus_tag="ACHJ48_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO4708902.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVVAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKENHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16195..16893)
                     /locus_tag="ACHJ48_003419"
     CDS             complement(16195..16893)
                     /locus_tag="ACHJ48_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO4708903.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARTLEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVAATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(16912..>17301)
                     /locus_tag="ACHJ48_003420"
     CDS             complement(16912..>17301)
                     /locus_tag="ACHJ48_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfI family protein"
                     /protein_id="HFO4708904.1"
                     /translation="PLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINILFTSR
                     QMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSS
                     LEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         4404 a         4604 c         4145 g         4148 t
ORIGIN      
        1 aaacattaaa caggtcacat tacaatgaca ttaccaacca ctatttattc atttcctgca
       61 tatttatctc gtttttcgtc caccgacaaa ccagtaaaat taaaatttca tcaatacgca
      121 cgcgctacat tactttcaaa tcgtggaaga gatcataatt gcgatggcag acgtactgtt
      181 gagatccaca aactagatct ctcagactgg caagcattca ataagttagc aacgaagtgt
      241 aatgcttacg atggagtaac aatgaatggt gataattctt ttggatggaa ccatgaagct
      301 acattagata atattcacgc ccagaaatac aataaagctt acgcgggtgc gaggttaacc
      361 gcagaactga aatatttatt gcaagatgtc gaatccttcg aacccaacag taaatacaca
      421 atacacgaag tggttttggg acctggttat ggtactccag actataccgg ccaaacgata
      481 ggatacgttg taacccttcc tgcccaaatg cctaattgct ggtcatctga actaccaaca
      541 atcgatcttt atatcgacca acttcgcact gttacaggcg tttctaatgc cctcggattc
      601 atcattgctg cattgctaaa cgcgtattct gatttacccc atgatttgaa aattggtctg
      661 cgttccttat caagttcagc cgcaatctac tcgggtttgg gtttcgagcg agtgccgcag
      721 gagcgagaca tcagttgcgc tcgtatgtat ctaacgccag caaaccatcc tgacttatgg
      781 acacaagaga atggtgaatg gatctacttg cgaaactaat acctgacgaa aaatagaaag
      841 caaaacgccg accagtggtc ggcgttttta cgtctcgttg aataacgaat tattcagcta
      901 ctacgtttac gatcactttc gcgaatactt cgctgtgaac ctggaagctc acttcgtgtt
      961 cgccagtggt acgcagaacg ccgttcggca gacgaacttc gctcttagcc acttcaacgc
     1021 cagctgcagt tacagcgtca gcgatgtcgc gagtaccgat ggaaccgaac agtttacctt
     1081 cgtcgccagc tttagacgcg atggtaacag tttccagtgc attgattttc tcagcgcgag
     1141 cattagcagc tgccagaact tcagccagtt tagcttccag ttcagcgcga cgtgcttcga
     1201 agaattcaat gtttttcttg gtagctggaa cagctttacc ctgtggtacc aggaagttac
     1261 gagcatagcc cgctttaacg tttacctgat cacccaggct acccaggttt gctactttat
     1321 caagcagaat aacttgcatt accttatcct ctcaaagtcg tattaatgga ccgtgaccga
     1381 ttactgatgg cgatcagtgt acggcagcag ggacaggtag cgagcgcgtt tgatagcgcg
     1441 agccagctga cgctggtatt ttgcacgggt accggtgata cggcttggga caatcttacc
     1501 gctttcggtg atgtagtttt tcagcgtagc gatatcttta tagtcgatct cttgaacgcc
     1561 ttccgcggtg aaacggcaga acttgcgacg acggaaataa cgtgccatat ggctagtctc
     1621 cagaatctat caattcaatc tgctcggcat gcaaaaccat tttgctcagt ccgttctttg
     1681 ccttgtggca tgaaatgaac ccctgaacgg ttatgcgact gccgaccgtt atactgtgag
     1741 taatggcctg gttttcgtgt ccgctaacaa taacgggcat ttgacaccat gcctgccggt
     1801 gaaagccggc ttcctcctgc acagaacgat gctcaagcac gaactggcag tgaggaattc
     1861 ctgatggact gacctttcga aggggagtcc tgcacacggt gccggacaac accagacggt
     1921 tggtcatcag aaattactct tcagaatccc cagcttcagc atcatcagcg gtttcgtttg
     1981 cgaaatcatc gcgacgctca cggcgctcgt ctttcgcttt aaccatcgga gatgcttcgg
     2041 taacagcgtg cttggtacgc ataaccatgc tgcggataac ggcatcgttg aagcggaagg
     2101 tagtttccag ctcatcgatc acttcctgcg gagcttcaac gttcatcaga acgtagtgag
     2161 ctttgtgcag tttgttgatc gggtaagcca gctgacggcg gccccagtct tccagacggt
     2221 ggatcttgcc ttctgcacca gtgatggcag cagtgtagcg ctcgatcatg cccggaacct
     2281 gttcgctctg gtcaggatgg accataaaaa cgatttcgta atgacgcatc gaattgctcc
     2341 ttacggatta ttcagcctcc tgtctgggtc agccgaatcc cggggaggca aggaacgtgt
     2401 taaaggtcgg ctgaaaaatg acgcgttata gtactgacat accccgctaa actcaaggcc
     2461 agccgacaaa taaatctcat ctttcaccaa agtggactaa cacgctgatt tgaagttcat
     2521 cacacttcat catggtgttt tttcgaacaa ctgcatcaga aatggtcacc atcaacttct
     2581 ttatcattga ttaaaattaa atcagaagat catcacagag gagagcacac aaagtcagcc
     2641 gtcgaggagg tatctatgtt cagtcgagtt ttagcccttc tggctgtgct tttgctaagt
     2701 gcaaatacat gggcagccat tgaaattaat aaccaccagg ccagaaatat ggacgatgtg
     2761 caaagcttag gcgtgattta tatcaatcat aatttcgcca ctgaaagcga agcacgtcag
     2821 gcattaaatg aagaaacaga tgcgcaaggc gcaacgtact accacgtaat tctgatgcgg
     2881 gaaccgggga gtaacggcaa tatgcacgcc agcgcggata tttatcgcta gcaccaggta
     2941 taaccaacga aacattgcca tagtttgctt tgcccccttc gcagggggct ttttttgaaa
     3001 aacggctgcg gaattagacc agttatctcc tgaggaagga aatttccgca gcgcgtgttc
     3061 tacttctgcc cgtaataagc gttggggccg tgcttacgca tgaagtgttt attcatcagg
     3121 aagctgtcga tgtgattgag ttgtggatta atactgcgcg caatccacgc cattttcgcc
     3181 acttcttcca tcactaccgc gttatgcacc gcgtcgtggg catctttccc ccaggcgaac
     3241 ggtccgtgct gatacaccac aattcccggc gtatgcagcg gctcggcgtt gcccagcgtt
     3301 tcgataatca ctttgccagt gttcagttca tactcaccct gcacctcttc ttcgcttaat
     3361 ccacgcgtac acgggatatc accaaagaag tagtcagcgt gcgtggtgcc taacgccggg
     3421 atcgccagcc ccgcctgcgc ccatgcggta gcatgagtgg agtgggtatg gacaatgccg
     3481 ccaagcgacg gataacgacg gtagagttcg agatgcgtcg cggtgtcgga agacggacga
     3541 tattcccctt ccaccacctt accgctcata tcgaccacca ccatatcgtc tgctttcatg
     3601 gtttcgtagg cgacgccgct gggcttgatc accaccagcc cgcgttcgcg gtcgatagcg
     3661 ctgacgttgc cccaggtaaa ggtcaccagc ccatagcgcg gcagatccat gttggcttca
     3721 aacacctgct gttttagctt ttgcattatg ccgcctccac catgcccgct ttcgccatgc
     3781 gcgctttcac ccaatcacgc gctttcgcca cttctgccgc cgggtcttcc gccgtttcgc
     3841 tccacatctc aatcaggtac ggcccgcaat agccactctg tttgagcgtt tcgaagcaac
     3901 gttcgaaatc caccacacct tcgccaaatg gcacgttttt gaagacgccc ggtttggtgt
     3961 ctttcacatg taccgcgacg atatgcccga ttccggcctg caactccatc tgcacatcgt
     4021 tgtcccacgc cgacaggttg ccgatatccg ggtagagctg gaaccacgga ttgttgagat
     4081 agtgcgcgta gcccagcgcc ttgctgatgg agttcatcaa cggataatcc atgatctcca
     4141 tcgccagcgt cacctgcgcg cggctcgcca tctcaacgct ctctttcagg ccgtcacgga
     4201 aacgacgacg cgtttcgtta ttggcttcct gatagtaaac gtcatagccc gccagttgga
     4261 tcacgcgaat accaacgtcc tgggcgaact ggatagcttt acgcataatc tccagcccct
     4321 gcgcccgcac cgcgtcatct tcactgccca gcgggaaacg acgatgagca gaaaggcaca
     4381 tggacggcac gcgcacgccg gtttcaacaa tcgcattcac cagcgccaga cgctgctcgc
     4441 ggctccagtc gaggcgcgac aggcgctcgt ccgtttcatc taccgacatc tcgacaaaat
     4501 cgaagcccaa cgtttttgcc agttgcaggc gttccagcca gcactccccg gcggggagcg
     4561 ctttttcata gatgccaagc gggatttgtt tggacaacat atccgctcct tagccccaca
     4621 gttcggcgat ggaacgtttg aactgacgtg cggcttccac cggagaagcg gcatcacgga
     4681 tactacggcc cgcgataaag acgtgaatcg gaatgccttt gaacagcggc agatcttcca
     4741 gcgccaggcc accagtgacg gtgactttga agcccatatc ggaaagacgt ttgatcgcgg
     4801 tgatgtccgc ttcgccccac gccacgcctg cggcctgcgc gtcacggctg cggtgataaa
     4861 ccacctgctg aatacctgcg tcgcgccact gttgcgcctg ttcccaggtc cagtaaccgg
     4921 tcagttcgat ctgcacgtcg ccgttaaact cttttgccac gtccagcgcg cctttggcgg
     4981 tgttgatatc cgcacagcaa attaccgtca cccagtcagc gttggcttcg aagcacatac
     5041 gcgaaaggat tttgcctgca tcggcaattt tggcgtctgc cagtacgatt ttgtgcgggt
     5101 agagcgcttt caggtcacga accgcacgca cgccttcgcc cacgcacaga atggtgccca
     5161 cttcgataat gtcgacttct tcggcaatca ggcgagtggt ttcgtaggcg ctatccatag
     5221 tctggttgtc cagcgcgact tgcaacatcg gtaatgacat gttcaattcc ttcttaagct
     5281 gccgcgttgg tgcggtcaat gagatccagt acttcctgct cggtacggca ggcgcgtaaa
     5341 cggtcaaaat tctcttcatc ttcaaacagg ttgacgatct gcatgatgcc cacttcctgg
     5401 tgagtattgg catcgaccgc cgccatggtg atgaggatat ccaccggatc gttgtcatcg
     5461 tggttgaact ccagcggctt tttcagcgtt accagagaga aaccggtttt cttaacgccc
     5521 tcttccggac gcccgtgcgg cattgccagg cccggagcga taacgaaata aggaccgaac
     5581 tgttcaacgc catccagaat cgcctggtag taacgcggct ctaccacatc tgccgccacc
     5641 agcaggtcaa cgccgatttt caccgcttcc tgccatgtct ctgcttcagc ctgcagacgg
     5701 atggatttat tttcagccag cgaatcacgt aatttcatgg cgcgtcctta cttcacgtcc
     5761 tgcgggaaat gctctttgat cacttccagc agtttcgggc caaagtcggc aggagagagc
     5821 atgttgcgca cgccaaccac gtatttgttg ccggtcacgg tgatttcgcc cgcaatgtgc
     5881 gtagaagcga tgatgatatc cgcgccactc aactcgcttt tgtactcgcc aaccgcgcag
     5941 ctgtttaccg tatggtcaat gtttgattgg gttaaaaact ggtccacttt catcttcatg
     6001 atcatggaac tgccttgtcc gttgccacac acagccagaa tacgtacggt cataatcaaa
     6061 actccttatt aagcagactg ttctgccagt tgtttttctg catcttcttc tgcgcgcagc
     6121 gcgcggccag cgaagaacat ataagccagt gcaattacaa tgatgacggc cataaaggcg
     6181 ataccgatgg agaagaagcc ttgcatcatc ggcggtgcca gaatcgacca gtccgccatg
     6241 cccatccagg cactcatacc ggtgagtttc accgcccaga cgcaaccaaa gatttcgatc
     6301 attcccatca ccagacaaat cttcagcgct gcacgccagc cgccgaagtg gttagcgaac
     6361 acgccgatgg tggcgttgga gaagaacatc gggataaagc ctagaataat cagaatggaa
     6421 gagccgcagg cgaccaggat gccaactgca atcagctgac cgatggtgcc ccacataaag
     6481 ccccagacca cggcgttcgg cgcgaagcta tagatagccg cacagtcaat cgccagaacc
     6541 gcacctggga tcaggcgctg ggagataccg ttaaatgctt cagagagttc cgccacaaac
     6601 atgcgcacgc cctgagtgat gatgaagatc gccaccgcga aggagaaacc ggtttgcagg
     6661 atgtagaccg tccagtgcac tttgcccgcc atcgcctgca cggtgtcgat accgaaggag
     6721 agcagaatgg cgccaaagaa gatggtcatc acaatcgccg tggagacgat gttgtcgtgg
     6781 aaaatgttca gccagcccgg cagtttgagg tcttcaacgc tctcctcttt tttgccgagg
     6841 aacggcgcga ctttataggc aatccatgat gcaaactgct gctggtgacc gatggagaaa
     6901 ccacagccat ccgtcacttc ctgagtcggc ttgtacatca tgttggaagt gatgccccag
     6961 tagagcgaaa ccagaatcgc ggtacagata atggtggtcc acatggagta gccgaagata
     7021 aacagcgtaa cggcaatcaa cccggcctgc tggaacatga tgtggccggt caacatgatt
     7081 gtgcgaatgc cggtaatgcg acgcaacagc acgtaacaga tgttcagtgc cagcgctaac
     7141 aatacggcgt aacccaccca gctataggca tcgcccatgc ggtcgatggt tgccatcatt
     7201 gaagcgtagg tatcggaaat tgcgccgtta atgccgtaga cttcggacat tttcgccacc
     7261 accggtttga aggtgctggt gaggatgccg gaccctgcct gcaacaacat gaaaccaatt
     7321 atggttttaa tcgtgccttt aataataacg ctgacacttt tgcgcagtag gatgtagccc
     7381 agacaggtca caatacccag caacaacggg gcattggtca tgacctggtt aaaaaatacg
     7441 gtaaagatgt tgtagaggat ctccataacg atctcctgaa gaagaggtaa ccgggtattc
     7501 cacacacccg gaatgttgtg cactcactct aattttcaaa agtaatcaca acaagattat
     7561 ttatgattaa atgtgacgcg acccgcaaat aattccacat ggatagagcg ggattcacgc
     7621 aacgaaacaa ttattcattt aaattcattt agttaattga attatacatt ccattaaatc
     7681 tttccatact caaaatcaac aacaacttcc ctggcaaaaa gtcaaaaaat ggtcattgcc
     7741 ttccagaaaa aatagtgtca ggattaatca aaaccaatca catattgatt cgaattgaac
     7801 tatcatgaag gtaaatggcg atgagtaaag tgaaaagtat cacccgtgaa tcctggatcc
     7861 tgagcacttt cccggagtgg ggtagctggt tgaatgaaga aattgaacaa gaacaggtcg
     7921 ctcctggcac atttgcgatg tggtggcttg gctgcaccgg gatctggttg aaatcggaag
     7981 gtggcaccaa cgtttgcgtt gatttctggt gcggcactgg caaacaaagt cacggtaacc
     8041 cgttaatgaa acagggtcac cagatgcagc gcatggctgg cgtgaaaaaa ctgcagccaa
     8101 acctgcgtac caccccgttt gttcttgatc cgtttgcgat tcgccagatc gacgcggtac
     8161 tggcgactca cgatcacaac gatcatatcg acgttaacgt cgctgctgcc gtgatgcaga
     8221 actgtgctga tgacgtaccg tttatcggac cgaaaacctg tgtggatttg tggattggct
     8281 ggggcgtacc gaaagagcgt tgcatcgtgg tcaaaccggg cgatgtagta aaagtgaaag
     8341 acattgaaat tcatgcgctt gatgctttcg accgtactgc actgatcacc ctgcctgccg
     8401 atcaaaaagc ggctggcgta ctgccagatg gcatggacga tcgcgcggtg aactacctgt
     8461 tcaaaacgcc tggcggctcc ctgtatcaca gcggcgactc ccactactct aactattatg
     8521 cgaagcacgg taacgaacat cagatcgacg tggcgttagg atcgtacggc gaaaacccgc
     8581 gcggtatcac cgacaagatg accagcgccg atatgctgcg tatgggtgaa gcgctgaatg
     8641 cgaaagtggt tatcccgttc caccacgata tctggtcaaa cttccaggcc gatccgcaag
     8701 agatccgcgt gctgtgggag atgaaaaaag atcgtctgaa gtatggcttc aagccgttta
     8761 tctggcaggt gggcggcaaa tttacctggc cgctggataa agacaacttc gagtaccact
     8821 atccgcgcgg tttcgatgat tgcttcacta ttgaaccgga tctgccgttc aagtcattcc
     8881 tgtaatctgt cgtttatacc ggatagcgtg gagaagttcg cgctatccgg cgtagtattt
     8941 cctggctatt tcaaatatca tctaaaaaaa tcaaatttta tcggaatagc tcatgactga
     9001 agcacaaaga catcaaatcc tcctggaaat gctcgcacaa ttgggctttg tgaccgttga
     9061 gaaagtcgtt gagcgtctgg gaatttcgcc tgccactgcg cgacgcgata tcaataaact
     9121 tgacgaaagc ggcaaactga aaaaagtgcg caatggtgca gaagctatta cccaacagcg
     9181 cccgcgctgg acgccgatga atctgcatca ggcgcagaat cacgatgaaa aagtacgtat
     9241 cgctaaagcg gcctcgcagc tggttaatcc gggcgaaagc gtagtcatca actgcggctc
     9301 caccgcgttt ctgcttgggc gggaaatgtg tggcaagcca gtgcaaatca tcactaatta
     9361 tctaccgctg gcaaattacc tgatcgatca agaacatgac agcgtgatca ttatgggcgg
     9421 acagtacaac aaaagtcagt ccatcacttt aagcccgcag ggcagcgaaa acagtctcta
     9481 tgccgggcac tggatgttta ccagcggaaa agggctgacc gcagaagggt tgtataaaac
     9541 cgatatgctg acagcaatgg cagagcagaa gatgctgagc gtggtaggga aactggtggt
     9601 actggttgat agcagtaaga ttggcgaacg cgcgggaatg ctttttagcc gtgccgatca
     9661 aatcgatatg cttatcaccg gcaaaaatgc taacccggaa atcctgcaac aactggaagc
     9721 gcaaggggtc agcattctgc gtgtttaaag atgctggcgg aaaaatgtca cggcagcatc
     9781 taacgcctca ggcgtaatgc ggtgacgcac gcctggctgc catgaacagg ttagcagttt
     9841 atcccgtccc gtctcgctta aggcctgctg caaacgtagt gattcgtcgg caggcacaac
     9901 gtcatcgtcg aggccatacc acagcagtag aggtctgtca ctaagttgtt ccaggtggtt
     9961 tgtcgcttcc cactctgcca gtggcgcgac aatgttattg aattcattct gctgtgctac
    10021 cgtttcaggt atcagcggtg gaaacagtga acgggcgagt gatgtaaaat agcccgatcc
    10081 catcatgctg gcggtacatc tcaccgtggg gtggcgagcg gtaatcccca gtgccgtcat
    10141 cgcgcccatc gacgcaccac cgactgccag acggtcatca agcagccact tttcttcggc
    10201 gattgccgca cgtaaagtag tgaattcctg catactttgt agcaagattt gccagaattg
    10261 atttaaccgc cgcgctgcgt caccactaaa acggctaccg tgatcgggcg catccggcat
    10321 gatcacccgc aaaccagcct gcgccagcgc aacggcaaaa tagctataca ccagactgga
    10381 cgaagtaaag ccgtgataaa aaatcacgca cggtaacggg gtatcttttt gtccgacagg
    10441 ataagcatga agaacgggaa tatctgccag ctcgcgtgat tctatttcaa tcatctgtct
    10501 ctcctttttg tctggtatga catgaagaaa tccttagcac aagtttctgc tggcggtatt
    10561 agttcataat gttgagatgt gggttacgct ttcgtcacgt tttcattcga aaatcgcgta
    10621 ccaggtaaca attcgggaac attcccccaa agttaaatta ataaggcact acactatggt
    10681 tagcaggaaa cgtaatagcg ttatttaccg gtttgccagt ttattattgg tgttgatgtt
    10741 aagtgcctgt agcgcactgc aaggtacgcc acagccagca ccgccagtaa cggaccatcc
    10801 gcaagagatt cgccgcgacc agacgcaagg gttacagcga ataggtagcg taagcacaat
    10861 ggttcggggt tctccggatg acgcattagc agaaatcaga gcaaaagctg tcgctgcaaa
    10921 agctgattat tacgttgtcg tcatggtgga cgaaaccatc gtgacaggac agtggtattc
    10981 acaagccatt ttgtaccgta aataaatcag acaacttaca tagtcagctt tacattgctt
    11041 tgcgtcccta tgcgtttacc tgtgcacaat aaattaccgg ctggaagcct gggcgtttat
    11101 taccgcgatg gaatgccctg caacatgtgg ggaaacgaaa aatggagctg acgatgaaac
    11161 aattacttgc ctcaccctcg ctgcaattag tgacttatcc tgcgagcgcc acggcacagt
    11221 ctgcagaatt cgccagtgct gactgcgtga cgggcctgaa tgaaataggt cagatctcag
    11281 ttagcaatat ttcaggggat ccgcaggacg tggaacgtat cgtggcgtta aaggccgatg
    11341 aacaaggtgc gtcatggtac cgcattatta ccatgtacga agaccagcag cctgataact
    11401 ggcgcgtaca ggcgatactg tatgcatgag tgtcatctac agcaaatgaa caatgccgga
    11461 tgcggcgcga gcgccttgtc cggcctacaa ttttcacata tttcatttag ttaatcgaaa
    11521 ccagcgtcgc atcagtcgta tacgcttaca cacacactcc ccccgtcgcc cgcagcaata
    11581 aatcattctg gatctgctct gacaaccgta cgccgccgcg cgtatctaac atcacctgac
    11641 accacgcctg cgccattggc ggagaagcat atttcaacat ttgcgcaccg cagcccagca
    11701 ggaatagctg atgagtaatc tctcgcccca gttcttcagc tggcttacgc agctgctgct
    11761 gtaaacgacg aaccgcgcaa tcaaaatagc gatcctgccc tttcacttct acaaatgctt
    11821 ccgacaataa gtcgtatacg cccgcttgct tattgagaac gcgcagcaca tccaggcaca
    11881 taatattgcc ggaaccttcc caaatactgt ttaccggcat ctcccggtaa agccgcggca
    11941 gctcgctctc ctcgcaataa ccaatgccgc ccagcacctc catcgcttcg gccacaaacg
    12001 gcatacctcg tttgcagatc acaaattttg ccgcaggcgt aaataaacgc gcccacagag
    12061 cttctttggc atcggcgcgc cggtcccacg ctcgcgcaag acgaaacagc aacgccgttt
    12121 gcccttcaag ctgaagtgcc atgcgactta agacatgacg cataaggggc tgttggatca
    12181 atggattacc aaaaacatgg cgttgatgcg catgataaat cgccagcgag aacgcacggc
    12241 gcatcatggc atggctaccc agggcgcaat caaaacgcgt catccccccc attttcagga
    12301 tcagacgaat tccttcccct tcctgcccca ataaccagcc aatggcatcc tgaaactcca
    12361 cttcacaact ggcgttagag cgattaccca gcttatcttt cagccgctcg aggcgaatcg
    12421 cgttgcgttg cccgtcaggc aaaaagcgcg gcacaaaaaa gcaggacaaa ccgcccgcgg
    12481 tctgcgccag caccagatgc gcatcgcttt gcggaactga gaaaaaccat ttatgcccca
    12541 ccagccgata agagccatcc tccagacgct ctgcgcgggt ggtgttgctc atgacatcgg
    12601 aaccgccctg cttttccgtc attcccatgc caatcaacaa accgcgtttt tgcccacctg
    12661 gcaataagtg agaatcgtag cgatcgctca acagcggtgt ggtccagtcc tgaaacggcg
    12721 cgggtaacat ctgtaacaac aatggcgtgg cggcaaaggt catggttatc ggacataacg
    12781 accccgcctc aacctgtgcg tgcaacataa aacgcgccgc gcgcgccaca aatgcgccgg
    12841 agcgagcgtc ttcttcccag gcaagattgt gcacccgatt ggtacatagt gcctgcatca
    12901 gcaggtgcca ggcggggtga aaacgcacat cgtccagacg gcgtccttgc gcatcgtagc
    12961 gcaacagttc aggcggattc acattcgcca gccgccccag ttcaagggat tcagccgttc
    13021 ctaactgctg accaatactg gctagaaaat cgctatccca gccagcacct tcacgcgtta
    13081 ccgcttcgca aagcgcgcca tcagacaggt ataagttgct gttatttaat ggtataggtt
    13141 gattaaaaac ggtgtgagtt tgccagtgca ctgtgtctcc ctccatcaat ggcagtcacc
    13201 attagtatgg tcactgccat ggattcatga cagaaagaga ttgctaaaac attcttatcc
    13261 agcaatgtaa tgcggaacga aacgtgaggt gtctttggtg atcagtgtgt tatcttcacg
    13321 gatccccatt ccgcacgctt catcatcgac aatccagcta ccgatgagtg tgtagctatc
    13381 gccaaaccgc ggcagaggtt gaaacgcctg gtagatcatc ggttcatcgg cgtaatcacc
    13441 atcagcgtgg tcaacgacgt tattctggcc gtcgaaaatg gtgacatttc cgccttcgcg
    13501 cgagtagatc ggtttacgca catagctttc gccagcggca atctgcggtt tttcaccctc
    13561 aaaccaggat gcaagaaggt taggatgacc agggaagaag cgccaaagca gcggcattag
    13621 ccctttatta ctcaagatac ttttccataa cggctccacc cattgctcgc gacgcttgcg
    13681 cagcagcgga ccgttatcgt cacgcatcat ccactccagc ggatacagct taaatgcacg
    13741 ctggatgaca ttatcatcaa gatcggtcag tacgccgccg acgcccaaac cgagatcttc
    13801 aatgtagata aaccgcgact cctgccctgc ctgctgggcg cagtcctgca agtacagcac
    13861 ggtactcctg tcttcatcgg tgtcctgaca gcagcaaaaa taaaacggtt cccgactgta
    13921 aagctcgcta aagcgtgaaa tcaggcgttc ctgaatagca ttgtactgat cggcatcacg
    13981 cggaataatg ccgctgcgcc gggcatcctc cagccacagc cactggaaat aagccgactc
    14041 gtacaatgaa gttggcgtat cggcgttgta ctccagcagc ttcaccggcg cattgccaca
    14101 ccaggcaaaa tccatccgtc catacagcga aggatcgcgg gcgcgccagc tttcagcgat
    14161 cacatcccag tacaacggcg ggatcgccag ttgcgtcagg atctcttcat cttttaccgc
    14221 gcgatccacc acctcaaggc acatctgatg cagttccgca gtcggttttt cgatctgctc
    14281 ttcaatctgg cgcagagtaa aacggtaagc ccgactctca tcccaataga tttcattgtc
    14341 gatgatatga aagtcgaaac cgttgtcagc ggcgatctgg tccagatccc gtcgcacagg
    14401 aacgttgtgt ctcagcatga ttagcctccc caatgcccac gggcgctgga agaacgacca
    14461 tagccgccgc gagaaacggt tgacgctttt ttggtggtaa agccgcctga agagtaagac
    14521 tcttttttgc cagaaccgga gcgccaggag taatcaccgg aagtgttgcg ccagacgggg
    14581 cgggaagcaa atgaggatcc gccgctgtta tagacaaacg gctcatcgcg atctttacga
    14641 ataacccggc ttaacaaaaa tccagaaacg accgggatcc aactctgttc aacattgtcg
    14701 tagtagcaat tttcgtactt agactgacag ttctgctgag tcatattctt cggaacatcg
    14761 gcataaaatg ccgctttggc gttgttccag ccacgcgcgc aaatgtcggc attattaccg
    14821 tcatcaatac aatcctgcac tgtcgaataa aacgttccgt cgccgtcgtt atcgacatcg
    14881 ccactatcgc cacaaccttt caatacaaaa aaagcggcac cgcccattag cgccaacgtt
    14941 aagtattttt gtgcgtagcg attgcgacgc ggttcaaaag gattattcgg tctaccaatg
    15001 cgactgatcg ctccgtgacc aattttactg ttatttcttg atttgcgttt tctggccatg
    15061 atcgtccatt accatgtcat acaagctgcg ttaaaaatac cgccagccag cgcggcggtt
    15121 cccataaaca ttcctgctgc ggtattgtga ttaataattt tttcgcttaa tgcgggcata
    15181 taaagtctga caccagcaaa aacgagtaac tgaatcacca gtgcaatccc gccccaggcg
    15241 aaatagtctg gaatactcac cgcattgatt gccgcactgg ataaggggat cacgtaaccc
    15301 aacaatgtac cgctgaacgc cagtgacgct gcggtattat tgtttttgat taattgccat
    15361 tcattgtgcg gtgtaatttt ggagtagata aacagaaaaa taatcaccat agccacgcca
    15421 ataaaaaaat aggcactaaa ggcaagaagt gaatccagta tatgcattgt aaacgtcctt
    15481 gtaattaacc aataatatga agtgatgtca gcgggatatc gacacccaac gcgcgcgaaa
    15541 ataaccactc tggctcgccg agatcgttaa aagattcctc accatttagc agcaaatatt
    15601 cgtaagtatc ttcggtcact tgccgctggt agcccatggt aaaattatgg acttcccatt
    15661 tagcatgatt ttgattttct actttttcca gcatgtagac gggttcgata tttcccggtt
    15721 cttcgctgtt aaaaaatcgc tgccagcgtt tttcctgcca gtttaaggtc attgccccca
    15781 tcgttttggc gttgatcgcc tcgcgccagt gattctcttt actgataccg tagctctctt
    15841 catagacaaa gagcttgata tcatcaatgt catcaatatc ttcgccgccg gtggtattga
    15901 tttgcagaaa ctcatcgcct gaagtgtagt agcggaaaat ctgactaccg ccgcccagat
    15961 cgatgtggct gacagcgact accgtaaatt cctcacccgg cagcgcaatc agcaattcat
    16021 cttccagcaa acgaaacgct aacgtgtcga gcgtaaagcc actattgaga tgaagtccca
    16081 gcggaccgcg agcgatagca ggcttattat ccttgccaaa cagacgctgg aaaaaaccag
    16141 acatatttta tccttaaaat agttgccagc cttgcagtga ggctggcaaa aaaattactc
    16201 gccctgttgg cgttgcagtc ttgctaatac atcctgggcg ctacttttat tgctaccgcc
    16261 aattccggct tccgccagct tttcgtcaag gtcgcgaccg tctgcgactt tttctaactg
    16321 tgcggcagca tccaggcgag cctgacgttc ggcctgacgc gtttgcaggc gttttaagga
    16381 ttctgctgct gtcgaaacgc tggaagatgc gccaacggta gaagtcgtta cagcctgttg
    16441 tgcacgctgc atggcttcag tggctttaac gacttccatt tgctgctcaa actgagcgat
    16501 acgctgcgct gtcgctgcaa ctgctttttc cacgccatca cgggagactt ccagattcga
    16561 caacacttgc tcttcagcgg taatgagatt ctcaaggcgc gcgatttctt cagcaacttc
    16621 gttaatcaac gacggattaa cgttcttgct taacgcttcc agcgtacggg cttccagact
    16681 ggctttgcgc tcacgtaaat ctttcagctt atcgtgactt aatttcaccc gcgccagcag
    16741 atcaacgcga gattttccgg ctttatcgag ttcagcttta gcgtcacgaa tatgctgttc
    16801 cagcatgcgc acaccctggg tttcttcaat ggattcttcc gcctgggaga taaacgattt
    16861 ccccagcgta aataaacttt ttaaaattcc cataaatacc cgtccttgta attagtgtga
    16921 atattcttct gtaatttcag ccagatccaa tgcgttatct accagcgagg ttatctcaag
    16981 caggatatct tcaagagaag atttaagcga taacgccccg aaaacaatgt aatactcttc
    17041 ttgttgtacg ctggagattc ctaccgatga caacggcatc atcttctgat ttcttaataa
    17101 gaaggtatta aattcatctg gattgctgat actactcacc ggacaaataa aggtttcgat
    17161 aatcatttgg cgggaagtaa aaagaatatt gatttgcaaa tcgccataat cattcatttt
    17221 aataattaat gcgttctcgc tatttgttac atcaatattt tgttcaggta cagtttgcag
    17281 cgccgtcgcc agcgccaacg g
//
LOCUS       DAZFDC010000099        17225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFDC010000099 DAZFDC010000000
VERSION     DAZFDC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.099"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            282..1625
                     /locus_tag="ACHJ48_003421"
     CDS             282..1625
                     /locus_tag="ACHJ48_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708905.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVAPLVPEEVHLLAAAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NAANDDINNTRQTIEDQPEADEPQQYVKLTVDDMRKWAAMDQQARNALQGVSGWCTRN
                     HFNIKNARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKW
                     CKERNLAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGG
                     AEKWSKKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1774..3282)
                     /gene="putP"
                     /locus_tag="ACHJ48_003422"
     CDS             complement(1774..3282)
                     /gene="putP"
                     /locus_tag="ACHJ48_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO4708906.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3441..3650)
                     /locus_tag="ACHJ48_003423"
     CDS             complement(3441..3650)
                     /locus_tag="ACHJ48_003423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708907.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            3705..7667
                     /gene="putA"
                     /locus_tag="ACHJ48_003424"
     CDS             3705..7667
                     /gene="putA"
                     /locus_tag="ACHJ48_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4708908.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7707..8345)
                     /gene="rutR"
                     /locus_tag="ACHJ48_003425"
     CDS             complement(7707..8345)
                     /gene="rutR"
                     /locus_tag="ACHJ48_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO4708909.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKSLIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8633..9724
                     /gene="rutA"
                     /locus_tag="ACHJ48_003426"
     CDS             8633..9724
                     /gene="rutA"
                     /locus_tag="ACHJ48_003426"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO4708910.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9724..10416
                     /gene="rutB"
                     /locus_tag="ACHJ48_003427"
     CDS             9724..10416
                     /gene="rutB"
                     /locus_tag="ACHJ48_003427"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO4708911.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTS
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10428..10814
                     /gene="rutC"
                     /locus_tag="ACHJ48_003428"
     CDS             10428..10814
                     /gene="rutC"
                     /locus_tag="ACHJ48_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO4708912.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10822..11622
                     /gene="rutD"
                     /locus_tag="ACHJ48_003429"
     CDS             10822..11622
                     /gene="rutD"
                     /locus_tag="ACHJ48_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO4708913.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDHP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11632..12222
                     /gene="rutE"
                     /locus_tag="ACHJ48_003430"
     CDS             11632..12222
                     /gene="rutE"
                     /locus_tag="ACHJ48_003430"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO4708914.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12233..12727
                     /gene="rutF"
                     /locus_tag="ACHJ48_003431"
     CDS             12233..12727
                     /gene="rutF"
                     /locus_tag="ACHJ48_003431"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO4708915.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQN
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12748..14076
                     /gene="rutG"
                     /locus_tag="ACHJ48_003432"
     CDS             12748..14076
                     /gene="rutG"
                     /locus_tag="ACHJ48_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO4708916.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGSSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14159..14266)
                     /locus_tag="ACHJ48_003433"
     CDS             complement(14159..14266)
                     /locus_tag="ACHJ48_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFO4708917.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            14638..15234
                     /gene="wrbA"
                     /locus_tag="ACHJ48_003434"
     CDS             14638..15234
                     /gene="wrbA"
                     /locus_tag="ACHJ48_003434"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4708918.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMS
                     PQLFEKAGGKTQTAPVATPQELANYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15255..15482
                     /gene="yccJ"
                     /locus_tag="ACHJ48_003435"
     CDS             15255..15482
                     /gene="yccJ"
                     /locus_tag="ACHJ48_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO4708919.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15520..16761)
                     /gene="agp"
                     /locus_tag="ACHJ48_003436"
     CDS             complement(15520..16761)
                     /gene="agp"
                     /locus_tag="ACHJ48_003436"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO4708920.1"
                     /translation="MNKTLIAAAVAGVVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWRDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(17052..>17225)
                     /locus_tag="ACHJ48_003437"
     CDS             complement(17052..>17225)
                     /locus_tag="ACHJ48_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708921.1"
                     /translation="SLGNESFSTDEDLEYGYLINTGNHYDVYLPPELFAQAYELNNKE
                     RNAQIGFLTRYAI"
BASE COUNT         4103 a         4580 c         4546 g         3996 t
ORIGIN      
        1 aggaagaaat tattatttaa cgcgatgata aaaggcgtaa aaaaaaataa taatcttcat
       61 gaaactcacc ccgatggcag cattgtaaat tcgccaataa ctggcattcc tcctgctttt
      121 tacgggtgtt tccattaaca ccagaatgag cggattgttt actacatcaa gaacgcatcc
      181 gaagtgtaca tcctggtgcg ccgactgaat cgcccggagc catgcggatg gctacgactt
      241 aatacgaatc attaaccctg taaggatacg gagagcatta catgagacca atatccaacc
      301 tcaatacgag cgcagcaacc tatattccac cgcaacaact cccgagtagt tgtctggaaa
      361 ctttgacgct tctggtgcag atgaataatt atatcaaaag ggatgcggac tacagcacgg
      421 gcatggctgt agctccactg gttcctgaag aggttcactt gctggctgct gcgatgacaa
      481 ccgaactgca tcgacatcag ttccaaccgg tggtaagtgc aaatgatctg caacttcctg
      541 aaccgttcac cttcgatgtt gccggtttca ccatcacctt cacgaagacg caagagcatg
      601 acaacacggg caatctcatg aaaattgcag tcagcaagag cggtgtgtgc accagtacaa
      661 atattactct tgagctgttt cactccattg tcacaaccct gatgtcacgc agtcaatatg
      721 gaacctttga tctctggtcc gtacggccaa tactcacgga tgaaagtcaa aacagggtgc
      781 atgaagcagc tcgctactcc ccggcccagc aatacggaag agaagaaaca cactttacca
      841 acaatggcat gcgagaattt ggaaatatgt ctccgctggc gtggcgtaac gatgtggaat
      901 tacagcaatc gtgtaataca agcaacatcc caccaaatgc tgcaaatgac gacataaaca
      961 acaccaggca gaccatcgag gaccagccag aggcggacga gccacagcaa tatgttaaat
     1021 tgactgtaga cgacatgcga aaatgggcgg ctatggacca gcaggcgcga aacgctctac
     1081 agggagtttc aggatggtgt acgcgcaatc attttaatat caaaaatgcc agaaattacc
     1141 tgaccgatca cggcctgaac tacgcgggac aggtaaaggt aaacaggccg catgaatatg
     1201 ccaaattcac ccttgaacat atccgccagt gggcagcctt aaacaagcat gtgcgaaaac
     1261 cggttggtta tttagaaaag tggtgtaagg aaagaaacct cgcgccaact actgccagga
     1321 attacctgaa gaacgacggg cttaccgccc tgggtgagtt gaagctgaca gggccgcaga
     1381 aatgggtcac gttcaccttc ggagacatcg tacaatgggc gaatatgacc caggaggagc
     1441 gtaacagcgc agggggagcc gaaaagtgga gtaaaaaacg cggcttccag tggtctactg
     1501 ccagatccta cctgaaatcg agcggagtga ccaagcaagg tgcgagaaaa ctggcgtggt
     1561 taaaaaacag cgggaatatg tcgaatccat tctatctcgc ccaaccaacc agccgcagga
     1621 cataagctga aggtaccgat agcggcaatt ttgttcatct gtgccgggtg ggcaaatagc
     1681 aattgccccc gaacactccc tgccaggcaa cttataccgc atggcaggga acattcgggt
     1741 taaaaagcaa aagataacta attactcttc agcttagcct tcctgcaacc gtgacggcgg
     1801 agccgaatga tagtgcgcat cggcctcggc aaagcgtttt tgcatcgccg ctgacggtgc
     1861 tttacccagc aaactaaaca ctacaatccc aatactgcca aagataaagc ccggaataat
     1921 ttcgtacagt cccagccagc cgaactgttt ccagacgatc accgtcagcg caccgatgat
     1981 catccccgcc agcgcaccgt tgcgcgtcat gcgtgaccac atcaccgaaa acagcaccac
     2041 cggaccaaac gccgcgccaa agcctgccca cgcgtagctc actaagccca gcacgcggtt
     2101 ttccgggttc gctgccaacg caatcgccac cagcgccacc accagcacca tcacacgccc
     2161 tacccacacc agttctttct ggctggcatg tttacgcaga aacgctttgt acaaatcttc
     2221 ggtaatcgca ctggaacata ccagcagctg gcaacttaac gttgacatta ccgccgccag
     2281 aatcgccgac agcagaatcc cggcaatcca cgggttaaac agaatttgcg ccagttcgat
     2341 aaacacgcgc tcggcgttct ggtttaccgc accagctacc gacgggtgct cgttaaagta
     2401 agcgatacca aagaagccga cagccaccgc ccctgccagg cagaggatca tccaggtcat
     2461 actgatacga cgcgcatgga cgatgctgtg gtgagaatcc gccgccataa aacgcgccag
     2521 gatgtgcggt tgcccgaagt aacccagccc ccaccccatc agcgagataa tggcgacaaa
     2581 gttcagccct ttgagcatat ccacgttttc gatgctcttt tgtttgatca cttccagcga
     2641 gtcaccaaag ccaccgacac tgataatgac gataaccggc gtcaggatca gggcaaaaat
     2701 catcaggctg gcctgtacag tgtcagtcca gctcaccgcg aggaaaccgc caataaaggt
     2761 gtaaaggatc gtcgccgcag ctcccgccca cagagccgtt tcgtagctca tgccaaaggt
     2821 actttcaaac agacgcgcgc ctgccacaat gcccgaagcg caataaatgg tgaagaacag
     2881 caaaatcacc agcgcggaga taatgcgcaa aatgcggctt ttatcttcaa aacgcccggt
     2941 gaaataatcc ggtaatgtta aggcgttatt gttgtattcg gtatgcacac gcaaccgccc
     3001 ggccaccagc ttccagttaa tccacgcgcc taatgtcagg ccaatggcga tccaactttc
     3061 ggaaatcccg gaaagaaaaa ccgcgcccgg caaccccatt aacagccagc cgctcatatc
     3121 cgatgcaccc gccgataatg ccgtcacgaa tggcccaagg ctacgtccgc ccagaatata
     3181 gtcgtcaaag tttttcgttg atcgccaggc gataaaccca atcaatatca tgccaaagat
     3241 atagacacaa aatgtcacca acatcggtgt gctaatagcc atctaaagtc tccaaaaaat
     3301 tattatcggc aatgtcgaaa cttgccgtta tatctgccac cggaacgggg taacagagtt
     3361 tatgttttac cagggcgacc gtatcctgcc ggaagcgctg gttattcaca atcgatttaa
     3421 cacaccattt acattaaatt ttagtgctca gcgacactat ttttcatcag gttgcactct
     3481 ctcacatttt ttgcggttgc acctttcaaa aatgttaact gccgcagaga aaaagtctga
     3541 gttatttttt taatccctgt catatcgatt tcttttatta acatttcatt catttttaag
     3601 cttgctacgc aggtcacatt taacatggtt gcacaaagtt gcaacatcat ggatatttca
     3661 cgataacgtt aagttgcacc tttcagaaca acaggagtaa tggcatggga accaccacca
     3721 tgggggttaa gctggacgac gcgacgcgtg agcgtattaa gtctgccgcg acacgtatcg
     3781 atcgcacgcc acactggtta attaagcagg cgattttttc ttatctcgaa caactggaaa
     3841 acagcgatac tctgccagag ctacctgcgc tgctttctgg cgctgccaat gagagcgatg
     3901 aagcaccgac tccggcagag gaaccacacc agccattcct cgactttgcc gagcaaattt
     3961 tgccccagtc ggtttcccgc gctgcgatca ccgcggccta tcgccgcccg gaaaccgaag
     4021 cggtttctat gctgctggaa caagcccgcc tgccgcaacc agttgctgaa caggcacaca
     4081 aactggcgta tcaactggcc gataaactgc gtaatcaaaa aaatgccagt ggccgcgcag
     4141 gtatggtcca ggggttattg caggagtttt cgctgtcatc gcaggaaggc gtggcgctga
     4201 tgtgtctggc agaagcgttg ttgcgtattc ccgacaaagc cacccgtgat gcgttaattc
     4261 gcgacaaaat cagcaacggt aactggcagt cacacattgg tcgtagcccg tcactgtttg
     4321 ttaatgccgc tacctggggg ctgttgttta ctggcaaact ggtttccacc cataacgaag
     4381 ccagcctctc ccgctcgctg aaccgcatta tcggtaagag cggtgaaccg ctgatccgca
     4441 aaggtgtgga tatggcaatg cgcctgatgg gcgagcagtt cgtcacgggc gaaaccatcg
     4501 cggaagcgtt agccaatgcc cgcaagctgg aagagaaagg tttccgttac tcttacgata
     4561 tgctcggcga agctgcactg actgccgcag acgcacaggc gtacatggtt tcctatcagc
     4621 aggcgattca cgccattggt aaagcgtcta acggtcgtgg catctatgaa ggtccgggca
     4681 tttcaatcaa gctgtcggcg ctgcatccgc gttacagccg cgcccagtat gaccgggtaa
     4741 tggaagagct ttacccgcgt ctgaaatcac tcaccctgct ggcgcgtcag tacgatattg
     4801 gtatcaacat tgacgccgaa gaggccgatc gtctggagat ctccctcgat ctgctggaaa
     4861 aactctgttt cgagccagag ctggcaggct ggaacggcat cggttttgtt attcaggctt
     4921 atcaaaaacg ctgcccgctg gtgattgatt acctgattga tctcgccacc cgcagccgtc
     4981 gccgtcttat gattcgcctg gtgaaaggcg cgtactggga tagtgaaatc aagcgcgcgc
     5041 agatggacgg ccttgaaggt tatccggttt atacccgcaa ggtatatacc gacgtttctt
     5101 atctcgcctg tgcgaaaaag ctgctggcgg tgccgaatct aatctacccg cagttcgcaa
     5161 cgcacaacgc ccacacgctg gcggcgattt atcaactggc aggtcagaac tactatccgg
     5221 gtcagtacga gttccagtgc ctgcatggta tgggcgagcc gctgtatgag caggtcaccg
     5281 ggaaagttgc cgacggcaaa cttaaccgtc cgtgtcgtat ttatgccccg gtcggcacac
     5341 atgaaacgct gctggcgtat ctggtgcgcc gcctgctgga aaacggtgct aacacctcgt
     5401 ttgttaaccg tattgccgat acctctttgc cactggatga actggtcgcc gatccggtca
     5461 ctgctgtaga aaaactggca caacaggaag ggcaaactgg attaccgcat ccgaaaattc
     5521 ccctgccgcg cgatctttac ggtcacgggc gcgacaactc ggcagggctg gatctcgcta
     5581 acgaacaccg tctggcatcc ctctcttctg ccctgctcaa tagtgcactg caaaaatggc
     5641 aggccttgcc aatgctggaa caaccggtag cggcaggtga gatgtcgccc gttattaacc
     5701 cggcggaacc gaaagatatt gtgggctatg tacgtgaagc cacgccgcgt gaagtagagc
     5761 aggcgctgga aagtgcggtt aataacgcgc caatctggtt tgccactcct ccggctgaac
     5821 gcgcggcgat tttgcatcgc gctgccgtgc tgatggaaag ccagatgcag caactgattg
     5881 gtattctggt gcgtgaagcc ggaaaaacct tcagtaacgc cattgccgaa gtgcgtgaag
     5941 cggtcgattt tctccactac tacgccggac aggtgcggga tgatttcgct aacgaaaccc
     6001 atcgtccatt agggcctgtg gtatgtatca gcccgtggaa cttcccgctg gcgattttca
     6061 ccgggcagat cgctgccgca ctggcggcag gtaacagcgt actggcaaaa ccggcagaac
     6121 aaacgccact gattgccgcg caagggatcg ccattttgct ggaagcgggc gtaccgccag
     6181 gcgtggtgca attgctgcca ggtcggggtg aaaccgtggg cgcgcaactg acgggtgatg
     6241 atcgcgtacg cggggtgatg tttaccggtt caaccgaagt cgctacgtta ctgcagcgca
     6301 atatcgccag ccgcctcgac gctcagggtc gccctattcc gctcatcgct gaaaccggcg
     6361 gcatgaacgc gatgattgtc gattcttcag cactgaccga acaagtcgta atagatgtgc
     6421 tggcctcggc gttcgacagt gcgggtcagc gttgttcggc gctgcgcgtg ctgtgcctgc
     6481 aagatgagat tgccgaccac acgttgaaaa tgctgcgcgg cgcaatggcc gaatgccgga
     6541 tgggtaatcc gggtcgcctg accaccgata tcggtccggt gattgatagc gaagcgaaag
     6601 ccaatattga gcgccatatt cagaccatgc gtagcaaagg ccgtccggtg ttccaggcgg
     6661 tgcgggaaaa cagcgaagat gcccgtgaat ggcaaagcgg cacctttgtc gccccgacgc
     6721 tgatcgaact ggatgacttt gccgaattgc aaaaagaggt ctttggtccg gtgctgcatg
     6781 tggtgcgtta taaccgtaat cagctgccgg agctgatcga gcagattaac gcttccggct
     6841 atggtctgac gcttggcgtt catacgcgta ttgatgaaac catcgcccag gtcactggct
     6901 cagcccatgt tggtaacctg tatgttaacc gtaatatggt gggcgcagtg gttggggtgc
     6961 agccattcgg cggcgaaggg ttgtccggta ccgggccgaa agcaggcggt ccgctctatc
     7021 tctaccgtct gctggcgaat cgcccggaaa gtgcgctggc ggtgacactc gcgcgtcagg
     7081 atgcagagta tccggtcgat gcgcagttga aagccgcatt gactcagccg ctaaatgcac
     7141 tgcgggaatg ggcagcaaat cgtccagaat tgcaggcgtt atgtacgcaa tatggcgagc
     7201 tggcgcaggc aggaacacaa cgattgctgc cagggccgac gggtgagcgc aacacctgga
     7261 cgctgctgcc gcgtgagcgc gtattgtgta tcgcggatga tgaacaggat gcgctgactc
     7321 agctcgccgc cgtgctggcg gtgggcagcc aggtgctgtg gccagatgac gcgctgcatc
     7381 gtcagttagt gaaggcattg ccatcgactg tcagcgagcg tattcaactg gcgaaagcgg
     7441 aaaatattac cgctcaaccg tttgatgcgg tgatcttcca cggtgattcg gatcagcttc
     7501 gcgcattgtg tgaagcagtt gccgcgcggg atggcgcaat tgtttcggtg cagggttttg
     7561 cccgtggcga aagcaatatc ctgctggaac ggctgtatat cgagcgttcg ctgagtgtga
     7621 ataccgctgc cgctggcggt aacgccagct taatgacgat cggttaaaca ggtagccgga
     7681 ggatgttaca ccctcctccg gcatcattag cgtggtcgaa tcccctcaat aataatccgc
     7741 tgcacgtttt caaccgtttg attgaaaaat acctcatcgc gcaacgttgc gcctgtcacc
     7801 gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg aagcccaaat cataaaaatc
     7861 aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga cccaaccggc aatcagcgcc
     7921 gatttctcat caattaatga cttcaaatcg cccgtcagtt catccattaa cagcggcgcg
     7981 cctgccagca tctccataca gaacaggcgc gaagcctgcg gataatcgcg tgagacttcc
     8041 agcttcagac ggatgtactc tttgatcgcc gccagcgggg cgaaatcttc acgaaacgct
     8101 tttaacggtg ccagccagat atcgagaatc tgccgcagca cggcaatata cagcgcctct
     8161 tttgacggaa agtaatacag cagattggtt tttgaaacac ccgccaactc tgcaatttgc
     8221 tccagccgtg tgccgtgaaa accgaattgt gaaaaagtgt ccagtgctgc gctaagaatc
     8281 gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg ttgttttcac tgcgccttgc
     8341 gtcatgcgct ctcccctctt gtagattggc aacaggatag caaaaccccc gcccggcctc
     8401 gaccttctgc actctcatcg cgcacagatg catgttttat gtgcaactgt tttgaccgtt
     8461 tagtccactt tttaccagac attttagata acgcgatgtt aacacattaa taacactccc
     8521 tttttaaaaa ctggcatccg ctttgcaaac aagccaatac gcggctgaaa aggatatgca
     8581 gaatgcagcg ccccgtctga ctttcacttt acgagacgaa gagaggttga tgatgaaaat
     8641 tggcgtattc gtacctattg gcaacaacgg ctggctcatt tcgacccacg cgccgcagta
     8701 catgccgacc tttgaactga ataaagccat cgtgcaaaaa gcggagcact accatttcga
     8761 tttcgccctg tcgatgatca aactgcgtgg ctttggcggc aaaactgagt tctgggatca
     8821 caaccttgag tcgttcacct tgatggcggg gctggcggcc gtgacctcgc gcattcagat
     8881 ttacgccacc gccgccacct taacgttacc tccggcaatc gtcgcccgta tggccgcaac
     8941 catcgactcc atctctggcg ggcgttttgg cgtcaacctc gtgactggct ggcaaaagcc
     9001 cgagtatgag cagatgggta tctggcctgg cgatgactat ttctcccgtc gttacgacta
     9061 tctcaccgag tatgttcagg tgctgcgcga cctgtggggc acgggaaaaa gcgattttaa
     9121 aggcgatttt ttcaccatga atgattgtcg cgtcagtccg caaccgagtg tccccatgaa
     9181 agtgatctgc gccgggcaaa gcgacgctgg catggcgttc tccgctcagt atgccgattt
     9241 caacttctgt ttcggcaaag gcgtaaatac acccacggct ttcgccccga ccgctgcgcg
     9301 gatgaaacag gccgcagagc aaaccgggcg cgacgttggc tcttatgtat tgtttatggt
     9361 gattgccgat gaaaccgacg atgccgctcg cgccaaatgg gaacactaca aagcgggcgc
     9421 ggatgaagag gcgttaagct ggctaaccga acaaagtcag aaagataccc gctccggtac
     9481 tgacaccaac gtccgtcaga tggccgatcc cacttcggcg gtaaacatca atatggggac
     9541 gttagtcggt tcttacgcca gtgtcgcgcg catgttagat gaagtcgcaa gcgtgcctgg
     9601 tgccgaaggc gtgctgttaa ccttcgacga ttttctgtcg ggaatcgaaa ccttcggcga
     9661 gcgcattcaa ccactgatgc agtgccgcgc ccatctccct gtgctgactc aggaggtggc
     9721 atgatgacga cattaaccgc tcgaccggaa gccattacct tcgatccgca gcaaagtgcg
     9781 ctgatcgtgg tggatatgca aaacgcctat gccacgccag gcggctactt agatctcgcc
     9841 gggtttgatg tctcaaccac tcgcccggtc attgccaaca ttcaaaccgc cgtgaccgca
     9901 gcgcgagcgg cagggatgtt gatcatctgg tttcaaaatg gctgggatga acagtatgtc
     9961 gaggctggcg gccctggctc accgaatttt cataaatcga acgccctcaa aaccatgcgt
    10021 aaacagccgc agctgcaggg gaaattgctg gcgaaaggct cctgggatta tcaactggtg
    10081 gatgaactgg tgccgcagcc tggcgatatt gtgctgccga agccgcgcta cagcggtttc
    10141 ttcaatacgc cgctggacag cattttgcgc agccgcggaa tacgccatct ggttttcacc
    10201 agcatcgcta ccaacgtctg cgtcgaatcg acgctacgcg atggcttttt tctggagtat
    10261 ttcggcgtgg tgctggaaga cgcaacacac caggcggggc cggaatttgt acagaaagcc
    10321 gcgttgttca atatcgaaac cttttttggc tgggtcagcg acgtcgaaac gttctgcgac
    10381 gcgctttctc ccacgtcctt tgctcgtatc gcttaaggag tttaacgatg ccaaaatccg
    10441 taattattcc cgctggcagc agcgcaccgc tggccccctt cgttcccggc acgctggctg
    10501 atggcgtggt gtatgtctcc ggtacgctgg cttttgatca acacaataac gtgctgtttg
    10561 ccgatgaccc aaaggcgcaa acccgccacg ttctggaaac tatccgcaag gtaatcgaga
    10621 cagcgggtgg cacgatggcg gatgtgacct tcaacagcat ctttattacc gactggaaaa
    10681 attacgccgc gattaacgaa acctacgccg agttttttcc aggtgataaa ccggcgcgat
    10741 tctgcattca gtgcggactg gtaaaacctg acgcgctggt ggaaatcgcc acaattgcgc
    10801 atatcgccaa gtgaggccgc gatgaaactt tcactctcac ctccccctta tgctgatgcg
    10861 cccgtagtgg tgttgatttc gggtcttggc ggtagcggca gttactggtt accgcaactg
    10921 gcggtgctgg agcaggagta tcaggtagtc tgttacgacc agcgcggcac cggcaataat
    10981 cccgacacgc tggcagaaga ttacagtatc acccagatgg cagcggaact gcatcaggcg
    11041 ctggtagccg cagggattga gcattacgca gtggtcggcc atgcgctcgg tgcgctggtg
    11101 ggaatgcagc tggcgctgga tcatcccgcg tcggtaactg tgctggtctg cgttaacggc
    11161 tggctacgaa taaacgccca tacgcgccgc tgttttcagg ttcgcgaacg attactgtat
    11221 agcggtggcg cgcaggcatg ggtggaagcg cagccgttgt tcctctatcc cgccgactgg
    11281 atggcggccc gcgcacctcg ccttgaggca gaagacgcgc tggcactggc gcattttcag
    11341 ggcaaaaata atttactgcg tcgacttaac gccctcaaac gcgctgactt tagtcaccat
    11401 gcggatcgca tccgctgccc ggtgcaaatc atctgcgcca gtgatgatct gctggtgcca
    11461 tcagcatgtt ccagtgaact tcatgccgcc ctgcccgata gccagaaaat ggtgatgcgc
    11521 tatggcggac acgcctgcaa cgtgaccgat cccgaaacgt ttaatgctct gttactcaac
    11581 gggcttgcca gcctgttaca tcaccgtgaa gccgccctgt aaggaattgc tatgaacgaa
    11641 gccgttagcc caggtgcgct tagcaccctg ttcaccgatg cccgcactca caacggctgg
    11701 cgggagacac ccgtcagcga tgagacgtta cgggagcttt atgccctgat gaaatggggg
    11761 ccgacatcag ctaactgttc tccggcacgt atcgtgttta tccgcactgc agagggaaaa
    11821 gaacgtctgc gcccggcgct ttccagcggc aatctgcaaa aaaccctgac cgcgcccgtc
    11881 accgctatcg tcgcatggga cagtgaattt tatgaacggt taccgcaact gtttcctcac
    11941 ggcgatgccc gcagttggtt tacctccagc ccacaacttg ccgaagaaac agcgtttcgc
    12001 aacagttcca tgcaggcggc ttatctgatc atcgcctgcc gtgcgctggg gctggatacc
    12061 ggcccgatgt cgggttttga tcgtcaacac gtagacgacg ctttttttgc aggcagcacg
    12121 ctgaagagca atctgctgat taatatcggc tatggcgata gcagcaaact ttttgcgcgc
    12181 ctgccacgtc tgtcctttga agaagcctgc gggctgttgt aaggagcaaa ctatgaacat
    12241 tgtcgatcaa caaacttttc gcgatgcgat gtcctgcatg ggcgcggcgg tcaatatcat
    12301 caccacggac ggtccagccg ggcgcgccgg gttcaccgcc agcgccgtct gcagcgtgac
    12361 cgacacgcct cccacattac tggtgtgcct gaatcgtggg gcgtccgtct ggccggtatt
    12421 caatgaaaat cgaacgctgt gtgtaaatac acttagcgcc gggcaggagc cgctttcaaa
    12481 cctttttggc ggcaaaacgc ccatggaaca ccgctttgcc gccgcccgct ggcagaatgg
    12541 tgtgaccgga tgcccgcaac tggaagaggc gctggtttcg tttgactgcc gtatcagcca
    12601 ggtggtgagc gtcggcaccc acgacatttt gttttgcgcc atcgaagcga ttcatcgtca
    12661 cgccaccccc tacgggctgg tgtggttcga tcgcagttat cacgcgctga tgcgccctgc
    12721 ttgttaacct ccttaaggag acagctgatg gcaatgttcg gtcttcctca ctggcagtta
    12781 aaatcgacct ctacagagag cggcgtggtc gcgccggatg aacgattgcc gtttgctcag
    12841 acggcagtaa tgggcgttca acacgcggtg gcgatgtttg gcgcgacggt gttaatgccg
    12901 attctgatgg ggctggatcc caatctttcc attttaatgt cagggatcgg cacgttactg
    12961 tttttcttta ttaccggcgg gcgcgtgccc agttatctcg gctccagcgc ggcttttgtc
    13021 ggcgtggtga tcgccgcaac gggctttaac ggtcagggca ttaacccgaa tatcagcatt
    13081 gcgctgggcg ggattatcgc ctgtgggctg gtttataccg tcattggcct ggtggtgatg
    13141 aaaatcggta cgcgctggat tgaacggcta atgccgccgg tggtgacggg tgcggtggtg
    13201 atggcgattg gcctgaactt agcgcctatc gcggtaaaaa gtgtatcggc ttcaggcttt
    13261 gatagctgga tggcggtaat gacggtgctg tgtattgggc tggtggcggt gtttacccgt
    13321 ggcatgatcc agcggttatt gattcttgta ggattaattg tcgcctgtct gctgtatggc
    13381 gtgatgacca acgttttagg gctgggaaaa gccgtcgatt ttactctcgt cagccatgct
    13441 gcctggttcg gtctgccaca tttttccact cctgctttta atggtcaggc aatgatgctg
    13501 attgcgcccg tggcggtgat tctggtggcg gaaaacttag gtcatctcaa ggcggtcgcc
    13561 gggatgaccg gacgcaatat ggacccgtat atggggcgag cattcgtagg cgatggtctg
    13621 gcaacgatgc tttctggttc tgtcggcagt agcggcgtca ccacctatgc tgaaaatatc
    13681 ggtgtgatgg ccgtgacaaa agtctactcg acgctggtgt ttgttgctgc tgccgtcatc
    13741 gccatgctgt tgggcttttc accgaaattt ggcgcgctga ttcataccat tccggccgcg
    13801 gtgattggtg gtgcatcaat tgttgtgttc gggctgattg ccgtcgcagg cgcaaggata
    13861 tgggtacaaa accgtgtcga tttaagccag aacggtaatt tgattatggt cgcagtaacc
    13921 ttagtgctgg gcgcgggtga ttttgctctc acgctgggcg gttttacgtt gggaggaatc
    13981 ggtacagcaa cctttggcgc gattttactc aatgcgttat taagcagaag gttggttgac
    14041 gttccaccgc cagaagttgt tcatcaggag ccataaacag aaaaccgccg gggttaccgg
    14101 cggtgatggc tttttgacga gcttaagaaa aaaatgcatt cgtcattgat tagcccggct
    14161 agttgtcgct tttgccgtga ctgctcttgc cacctttttt acctgcttct gatgctcttt
    14221 cgcggtcttc tgcaaaattg ccggaaccgc ctcgatggtt tgccattact gacctccgtc
    14281 tgggtataga catcatattg ctagaattaa cgcggcgtat ggcggctgcc atattttcgc
    14341 tacgtgggac taagcttagt gaactgacgt gtttctaccc gcgtttggta atatcctgca
    14401 acagcttctt agcgagaata gatatcatct ccaataatta tccataagcc gctcttatga
    14461 ttaaaaaaac gcgatgagat aaaaatgtgt cattttgcga caaaattgcg tgcttgtgaa
    14521 agttgttata aatcaaataa gtggttgtga aatttgcact ctgaaaagga cgtcttatct
    14581 ttaaataagt ggtagcgaat cgctacggaa tagagataac acgaggagtg gttagaaatg
    14641 gctaaagttc tggtgcttta ttattccatg tacggacata ttgaaacgat ggcacgcgca
    14701 gtcgctgagg gtgcaagcaa agtggatggc gctgaagttg tcgttaagcg tgtaccggaa
    14761 accatgtcgc cgcaattatt tgaaaaagca ggcggtaaaa cgcaaactgc accggttgca
    14821 accccgcaag aactggccaa ttacgacgcc attatttttg gtacacctac ccgctttggc
    14881 aacatgtccg gtcaaatgcg taccttcctc gaccagacgg gcggcctgtg ggcttccggc
    14941 gcactatacg gaaaactggc gagcgtcttt agttccaccg gtactggcgg cggtcaggaa
    15001 caaaccatca catccacctg gacgaccctt gcgcatcacg gcatggtgat tgtccccatt
    15061 ggctacgcag cgcaggaatt atttgacgtt tcacaggttc gcggcggtac gccgtacggc
    15121 gcaaccacca tcgcaggcgg tgacggttca cgccagccca gccaggaaga actgtctatt
    15181 gctcgttatc aaggggaata tgtcgcaggt ctggcagtta aacttaatgg ctaatcttca
    15241 acaggaggat acgcatgcca actcaagaag cgaaagctca tcacgtcggt gaatgggcat
    15301 ctctgcgcaa tacgtcgccg gaaatagccg aggccatttt cgaagtcgcc gggtacgacg
    15361 aaaaaatggc ggaaaagatt tgggaagaag gtagcgatga agtgttagtc aaagcctttg
    15421 ctaagaccga caaagattcg cttttttggg gcgaacagac catcgaacgt aaaaacgttt
    15481 aaccagcgac tcccccgtag cgaacggggg agttttctgt tatttcaccg cttcattcaa
    15541 cacgctatca aacttatcca tcgggcagaa accatcagcg tctatcgggc aaccgcttaa
    15601 ttccagcgtc acgcgctgcg caggtgcctg cagggttaac gcatcggcat tacgtaactg
    15661 ttccgcactc tggtacacat attcaatttt catcaaatcg cgattggctt tgctgtcacg
    15721 ccaacgctgg aaaacgattt tgccgccaat cggcgtgcgt tcgttctggt catgcaactg
    15781 atacggtttg aaatccagcg ccgttaacag agaggcaatg ttggagtcat gcccaactaa
    15841 caccgtgatt tttggagcac tggtgcgatc ggtgaccagg gctttgtcga tataactgac
    15901 cagcggtttc gcaacattgc gcgccacttc cggtgaggta aacaggctgt cctggtaacc
    15961 gtttttcagc ttcgacaaca ccttccactg ctggtcagat ttgatttctc cccaggccac
    16021 ctgatccatc ggaaaacctt cgtagtattg caaagtaaac gcatctacca gcgagttgcc
    16081 gactttcagc ggcccggaaa cacctggttc ttgttgatac ttggcgctaa aggtattttt
    16141 gccatccacc agcgaacact gttgtttctc tttacaggca ggggaatctt tatagttaac
    16201 gattttttcc agtagctggt agctgtcggt aagctggagt ttgctgagct ctttctccat
    16261 tgccgccacc gcctgttcac tgaatgcggc ggaatcatcg gtgatcaccg ggttaaaggt
    16321 tgggtccata gtgcccattt tttcctggtg atgcacagga atatcacacc ccgggaatgc
    16381 gccggtaata aagaactgtg cggtcgcaac ggtacgttgc agactattgg cataggcata
    16441 aacggtgtcc ggcggcgggc attcccccga ttttaccatc ccctgctctg ccagccattc
    16501 acgcatgtaa tggcccatat acacttcgag tacgccacct ttggtggtga gttgcccacc
    16561 ggggacgtcc cattcgggcc atttattcgg cgtcgactgc tccagcacac tgccattgtt
    16621 cgccagcggc gcacgtaagt tatggcggct catcatgagc acttgctgta gctgatagcc
    16681 ttccggtacg gtttgtgcct gagcgtttga agcgagtaaa actacccctg ccacagctgc
    16741 ggcgattagc gttttgttca ttcctggcac ctcttttgtt atcaatcact aaagagtgtg
    16801 acagaaatat gacttttttc cgggaacagg ttcgcaaaat ggcttgttga acggctgttt
    16861 ttatattcat ctttaaaaac aggcaaggtt tgacgcaaac tatgcctgat tagcccctgc
    16921 ctcgcgtacg ccgctgatga ttgcacctgt ggctaaacag aatatcggcg gcgttaccaa
    16981 tacagaggca atatttacca ataaaaaagt gtgagactaa taacaatggt ttttagcata
    17041 tgcctctgtc attaaattgc ataacgagtt aaaaaaccaa tttgcgcatt cctttcctta
    17101 ttgtttaact cgtaagcctg agcaaaaagt tcaggaggga ggtaaacgtc ataatgattg
    17161 ccagtgttta ttaaataacc atactcaaga tcttcatctg ttgaaaagga ttcattccct
    17221 aagga
//
LOCUS       DAZFDC010000100        17217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFDC010000100 DAZFDC010000000
VERSION     DAZFDC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.100"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(52..783)
                     /gene="rlmB"
                     /locus_tag="ACHJ48_003438"
     CDS             complement(52..783)
                     /gene="rlmB"
                     /locus_tag="ACHJ48_003438"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO4708922.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(964..3405)
                     /gene="rnr"
                     /locus_tag="ACHJ48_003439"
     CDS             complement(964..3405)
                     /gene="rnr"
                     /locus_tag="ACHJ48_003439"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO4708923.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLMLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3444..3869)
                     /gene="nsrR"
                     /locus_tag="ACHJ48_003440"
     CDS             complement(3444..3869)
                     /gene="nsrR"
                     /locus_tag="ACHJ48_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO4708924.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4074..5372)
                     /gene="purA"
                     /locus_tag="ACHJ48_003441"
     CDS             complement(4074..5372)
                     /gene="purA"
                     /locus_tag="ACHJ48_003441"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO4708925.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5476..5673)
                     /gene="yjeT"
                     /locus_tag="ACHJ48_003442"
     CDS             complement(5476..5673)
                     /gene="yjeT"
                     /locus_tag="ACHJ48_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO4708926.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5755..6759)
                     /gene="hflC"
                     /locus_tag="ACHJ48_003443"
     CDS             complement(5755..6759)
                     /gene="hflC"
                     /locus_tag="ACHJ48_003443"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO4708927.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6762..8021)
                     /gene="hflK"
                     /locus_tag="ACHJ48_003444"
     CDS             complement(6762..8021)
                     /gene="hflK"
                     /locus_tag="ACHJ48_003444"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO4708928.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8107..9387)
                     /gene="hflX"
                     /locus_tag="ACHJ48_003445"
     CDS             complement(8107..9387)
                     /gene="hflX"
                     /locus_tag="ACHJ48_003445"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO4708929.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9464..9772)
                     /gene="hfq"
                     /locus_tag="ACHJ48_003446"
     CDS             complement(9464..9772)
                     /gene="hfq"
                     /locus_tag="ACHJ48_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO4708930.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9858..10808)
                     /gene="miaA"
                     /locus_tag="ACHJ48_003447"
     CDS             complement(9858..10808)
                     /gene="miaA"
                     /locus_tag="ACHJ48_003447"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO4708931.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLGGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10801..12648)
                     /gene="mutL"
                     /locus_tag="ACHJ48_003448"
     CDS             complement(10801..12648)
                     /gene="mutL"
                     /locus_tag="ACHJ48_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO4708932.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12658..13995)
                     /gene="amiB"
                     /locus_tag="ACHJ48_003449"
     CDS             complement(12658..13995)
                     /gene="amiB"
                     /locus_tag="ACHJ48_003449"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO4708933.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVVKRVETPTVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANNGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14014..14475)
                     /gene="tsaE"
                     /locus_tag="ACHJ48_003450"
     CDS             complement(14014..14475)
                     /gene="tsaE"
                     /locus_tag="ACHJ48_003450"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO4708934.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14447..15994)
                     /gene="nnr"
                     /locus_tag="ACHJ48_003451"
     CDS             complement(14447..15994)
                     /gene="nnr"
                     /locus_tag="ACHJ48_003451"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO4708935.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVALIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15993..17132
                     /gene="queG"
                     /locus_tag="ACHJ48_003452"
     CDS             15993..17132
                     /gene="queG"
                     /locus_tag="ACHJ48_003452"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO4708936.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17115..17168)
                     /locus_tag="ACHJ48_003453"
     CDS             complement(17115..17168)
                     /locus_tag="ACHJ48_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708937.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3880 a         4941 c         4337 g         4059 t
ORIGIN      
        1 agacaaaaaa aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg
       61 ctgacgaacc gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac
      121 acttcctgcc attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat
      181 accttcacct tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tttgatagag
      241 cgtatgatcc gcctcacctg ccgtaccgac gatccagata ttctcttcct gcaacatacg
      301 catggtgcgc gcaaggttag tcacccgaat cagtggaacg ctttctgccg cgccgcaggc
      361 tacttttttc gccgtggcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcgtg
      421 aacgccagca gcgtccgcgc tacgcaggca cgcgccgagg ttgtgcggat cggttacacc
      481 gtcgaggatc agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc
      541 ctggtactga cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct
      601 tttctcgtcg agatattggc ggtttgccaa ctggataacc acgccctggg actcaagggc
      661 gtggatcagc ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa
      721 acgttcaggg gcgcgctcca gcagggcctg cactgcatgg atgccgtaaa tcatttcgct
      781 cattaatgta ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gtctacatga
      841 tccctgcaat atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat
      901 ccggcatgaa caaagcgcat tttgtcagca atctaaccct cttctgtttg aagagggtaa
      961 tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cagctatttt
     1021 ctgcgttttc gccgatggct ttttcgcttt tctcgcgtct ttcttcgctg ctttcggctt
     1081 cgtttttttc tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg
     1141 acgacgcttg ccgccttttt tacctgcatc gccttttttc gctttctcgc gcgccgtttt
     1201 accgacgttg cgcggtgcgc gttcgctgga gatcaggcta aagtcgattt tgcgctcgtc
     1261 catattaacc gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctggccgct
     1321 ggattcaccc atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga
     1381 agagacatgg accagcccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc
     1441 agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca
     1501 cttcagccag tcagacacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca
     1561 gtgctgaccc aattgcagca tctcttccat cgaataatgg tagccaccgg tttcagtggt
     1621 gttgccctga tgcccctgct ctttcgccag cagatactta atggcgcggt gcagcatcag
     1681 gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccaggccaaa
     1741 gtgaccacgg ttttccggat cgtaaatcgc ctgtttcatc gagcgcagca gcatggtttg
     1801 cagcatttct gcatcaggac ggtcggcaac cgactccagc agctccgcgt agtcgcgcgg
     1861 ttccggcttg ttaccgcccg gcagttccag ccccagctct gccagcactg aacggaaaga
     1921 ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt
     1981 ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaacttgtg
     2041 tgcgtcgtta cgctgagtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa
     2101 cttcgcttct tcgctctcaa atgagatccc gccgcgttct tcacgggctt tatccagcac
     2161 tttatagagg ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag
     2221 atcctgatcg ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct
     2281 catcaccgct tcgtagaatt tgtagccagt caggcggcct ttcgacgaaa ccgtcatctc
     2341 gcacaccata cacaggcggt ctacctgcgg attgagcgag cacagaccgt tagagagcac
     2401 ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtaccac ggttacgcgc
     2461 ttctctgtcc agcggcgttg gcggacgcac atagtagctg acgtcagcaa tcgcgaccca
     2521 taaacgccag ccgccgccgc gttttttctc gcagtaaacc gcatcgtcaa agtcacgggc
     2581 gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc ccgcttttgc
     2641 ttcttccggc acttcttctt tcagcccagc aacctgttgc tcaacagcct gcggccagat
     2701 gtacggaatt tcatgggtac gcagagcgat atcaaccgcc atgcccgtgc ccatattgtc
     2761 gcccagcact tcgacgattt tacccaccgc tttggtacgg cgagtcgggc gttgggtcag
     2821 ttcaacaacc accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat
     2881 atcgaagctc agacggctat cgtcaggaac cacaaagccg acgcccgctt cggtaaagta
     2941 gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg
     3001 acctttacgg tcagcaccca gcggctgagc cagcacctga tcgccatgaa tgcaggtttt
     3061 catctgctcg ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta
     3121 gccatcacgg tggccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata
     3181 gcactgacga cgagtaaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg
     3241 caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct
     3301 ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt
     3361 cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt
     3421 gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc
     3481 tcttcaacca aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc
     3541 ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa
     3601 ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc
     3661 ggtttaccca ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga
     3721 ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact
     3781 tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat
     3841 ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat
     3901 acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg
     3961 aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc
     4021 aatgtatcac actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg
     4081 tcgaacgggt cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg
     4141 atcggtacac cggtcagctc ttcaatgcgc ttgatgtagt tcagcgccgc ctgcggcagg
     4201 ccgctacgat ctttcacgcc gaaggtggat tcagaccagc ccggcatggt ttcgtaaatc
     4261 ggctctacac ctttccagtc gtcagctgcc agcggagtgg tagtcacttc acgaccatcc
     4321 ggcatacggt aagccacaca gagtttcacc tctttcaggc catccagaac gtccagttta
     4381 gtcaggcaga agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc
     4441 agccagccgg tacgacgacg acgacccgta gttgcgccga attcgttacc ctgcttgcag
     4501 aggaactcgc cagtttcatc aaacagttca gtcgggaacg gacctgcacc cacacgagtg
     4561 gagtaagctt tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg
     4621 gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg
     4681 atatccagca gcgtaccctg cgcaccttcg aacatgacga aatcgccacg ctgacgcgcc
     4741 tggtcgagca ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc
     4801 gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg
     4861 aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg
     4921 tcgccaacac gcagaccgcg acgtgccact ttatcttcat aagcaggccc gataccacga
     4981 ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagcgcaaca
     5041 tgataatcaa ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg
     5101 ccacggtctt ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg
     5161 ttaccgatga tgctagttac attctcgcgg agaatacctg atggaataag atggagaacg
     5221 gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca
     5281 acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat
     5341 tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat
     5401 ggattctacc atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat
     5461 cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc
     5521 aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga
     5581 gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac
     5641 cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc
     5701 accgacatcc ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg
     5761 cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat
     5821 cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta
     5881 gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc
     5941 accgcgcatg atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc
     6001 ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac
     6061 cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt
     6121 gatctgctta attcgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt
     6181 gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc
     6241 gttatctgcc gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc
     6301 gtcacgtact tcgagggtca gacgaccacg ggaatcggtg acgatgtctt tcacgtccag
     6361 gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg
     6421 cgaaatgtcg cccccccccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt
     6481 gatgtaagag tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt
     6541 gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa
     6601 atgcagaccc ggctcataaa ccagaggttt gttgtcatcg tcacgcagta ccttaccaaa
     6661 acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac
     6721 cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg
     6781 tagtcgttac gctgcgcatt ggcgcggcgt tggtccataa tatcgccctg actggtggac
     6841 gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg
     6901 ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac
     6961 ggcagaacca tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttacccaac
     7021 actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat
     7081 tccggcagaa gtttagcaaa acgcgccact tcaccctgag cttccaggat ggtctgggcc
     7141 ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact
     7201 tcgttggtat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc
     7261 gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc
     7321 agcagcgtga tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg
     7381 ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact
     7441 ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac
     7501 agatattttt ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc
     7561 tcgtccgacg tcagcatcac accagaagct gccagttcac gcacggcttc cacgttcacc
     7621 ggtttgactt cgtcgataaa cgtcggtttc cagttcagac ctggctcaac cagatggctg
     7681 aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg
     7741 gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc
     7801 gggcgcgggc cttgcgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga
     7861 ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg
     7921 cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg
     7981 tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt
     8041 tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct
     8101 acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc
     8161 aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc
     8221 tattgcctga agctgataaa aacgacttct cagacgccct tcctgcggtg gcagacgcaa
     8281 tgtatgctgc gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat
     8341 ccccgctccg gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc
     8401 aatacgcggt tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat
     8461 ctcgtgagcg tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg
     8521 cacatccgcc gcatcaatca catgcagcag caatgtggct tgccgcgtct cttgtaacgt
     8581 ggctttaaat gctgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc
     8641 aagtacggtt tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc
     8701 aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt
     8761 accggcgttg gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg
     8821 ccgcccctgc tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg
     8881 attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaaccaat
     8941 cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgtgtag ccagatggcg
     9001 cagctgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat
     9061 atctaaaata aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg
     9121 ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcacccg tcgctttgac
     9181 agcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt
     9241 acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa
     9301 ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg
     9361 ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac
     9421 gcaggatcgc tggctccccg tgtaaaaaaa acagcccgaa accttattcg gtttcttcgc
     9481 tgtcctgttg cgcggaagta ttctgcgcgc tgctaccatg atggtagtta ctgctggtac
     9541 cgccaccggc gttgttactg tgatgagaaa ccgggcgaga cgggacaaca gtagaaatcg
     9601 cgtgcttgta aaccatctgg ctgaccgtgt ttttcaacag gatcacgaac tgatcaaaag
     9661 actcgatttg cccttgcagc ttaataccat tcaccaaata aatagaaact ggaacacgtt
     9721 cccgacgcag tgcgttcagg aacggatctt gtaaagattg ccccttagcc attctctctt
     9781 ttccttatat gcttatttgt actttgaacc tttcgattct gaaaaaattg cgcacgatac
     9841 gtctcaattg tacacattca gcctgcgata gcaccaacaa cctgtaatac ttcgtcacgc
     9901 gcctgttctg gtttttcact gtcaagccag tgaacccctt cccaaccacg cagccaggtt
     9961 atctgccgct tcgccaactg tctcgtggcg caaacacctc gataaaccat ttcatcgtat
    10021 gagatttcgc ctccaaggta agaccacatc tggcgataac ccacgcaacg aatggaaggc
    10081 aagtccgtat gcaaatctcc tcgggcaaaa agcgcccgga cttctgcttc aaaacctgaa
    10141 gccaacatct gatgaaaacg ctgctcaatt cgttgatgga gcagttcacg gctcgccggg
    10201 gcgatggcga actgatgcac ctgatacggt agagcgtctc ctgacgtttg cgtcagttcc
    10261 gttaaagttt tacccgaaat gaaaaaaact tccagtgccc gggaaagcct ttgtggatca
    10321 tttggatgaa tccttgctgc cgcaaccgga tcgatctcct gaagttgacg atgcaatgac
    10381 tcccaacctt gctctgccgc ctgttgctca attctggccc gtacttccgg gtctgccgac
    10441 ggtagcggcg acaacccttc cagcaatgcc ttgaaataca acatcgtacc gcccactaac
    10501 agtgggatcc gccccgccgc ggtgatatcg gccatttccg ccagcgcatc gcggcgaaaa
    10561 tcagcagccg agtaagcctg cgacggatcg cgaatatcca gcaatcggtg cggcgcggcg
    10621 agtaactctt cagcgttcgg cttcgccgtc ccgatatcca tccctttgta aataagggca
    10681 gaatcaacgc ttatcaactc tactggtaaa attttacgca gctcaatggc taacgccgtt
    10741 ttaccggagg ccgtcggccc catcaaaaaa atcgccttag gcaggctcgc cttactgata
    10801 tcactcatct ttcagggctt ttatcgccgg atgtaaatca acagattgta acagaccacc
    10861 cggcggcgtt ttcacaagtt gcggacataa ccgttccacg tccgccagca gggttatggc
    10921 ctgtgccatt gaccactgcg catgttcgct catcagattt cgtgcaatcc actgcgcaat
    10981 attgccaggt tcgaatacgg actgcttcgc taggtagcct atcagttcag gaatcaagat
    11041 ttgtaaattt tgttggcgta agggtaaagg cacggccctg atggtcacat gctgtgcatc
    11101 tgactggaaa tcaataccca attccgccag ggcagactgc gctttttcta atgccgattt
    11161 ttcttcgcca gaaactttta gccgcaacgg aatcagcaac ggctgggcgc aaacgggcgc
    11221 ttcacccggc gtcagttgta cctgacgcag ccaacgttct gccactggca aggctaaaag
    11281 tgaaatgttg ccgtcgcgct ccagcaacgc acagtcggaa tggacgatag tcagtacccg
    11341 accaaaactc tgactgttcg ccgcaagtgc aggttcctgc ggttccggcg cttttggttt
    11401 ttgcatcggc gcgggcgttt gcaaaagctg gcgatacact tcaccttgct gtttctggta
    11461 acctggctgc gcattcggcc agggggcagc cggacgacta cctgatgctg gcgcaggagt
    11521 gtagcgcgga gctactggct cacgaactgc cggttcagca aagtgattgc gccccgccgc
    11581 cacgcggttt tccggaatgg gacgcggtgc aggttggggt tcatcgtcca gcggtagcgg
    11641 cgtttccagt tgctgttgta gcacgctcag cacgccctga tagataaagt catgcaccag
    11701 acgcgactga tgaaaacgca cttcgtgttt ggcggggtgc acgttgacgt ccacctgatg
    11761 cgggtcgatc tccagataca acacaaatgc aggttgctga tcggccccca gtttgtcttc
    11821 acaggcctgg cggatcgcgt gattgatcag gcgatcgcgc atcatgcgac cattcacgta
    11881 gcaatactga atttccgcca gtgcgggcgt ggtgtgattt ggatcggcca cccagccgcg
    11941 cagcgtgaga tcgccgtgtt gccattcaat cgccagcgct tgttcgagaa aagcggtgcc
    12001 acaaatcgcg cctaagcgcc gttctttttg cccgccttcc ggcaccgcgc ggtactggcg
    12061 tacgattttg ccgttatgcg acaggttgat cgtgacgtcg aaacgcgcca gtgcgatgcg
    12121 gcggatgatt tcatcgatat ggttaaattc ggttttctcg gtgcgcagga atttgcgccg
    12181 cgccggggtg ttgtagaaca ggtccagtac ctccagcgtc gttcccaccg gatgcgccgc
    12241 cggtttaacc gtcacgtcca tatcgcgccc ttcggcatag gcctgccagg cttcctgctg
    12301 ttctgcggtg cgtgaagtga gcgtcaggcg ggaaaccgaa ctgatactcg ccagcgcctc
    12361 accgcgaaag cccaggctga taatggcttc gagatcgtcc agagaggcga ttttactggt
    12421 ggcatgacgc gccagcgcca gcgccagctc gtcttttttg ataccacagc cgttatcacg
    12481 aatgcggata agtttcgccc caccgcgttc gatatcaata tcgatacgcg tcgcacctgc
    12541 atcgaggctg ttttccacca gttctttgac taccgacgca ggtcgctcga ccacctcacc
    12601 tgcggcaatc tggttcgcca gttgtggcgg taagacctga attggcatca atcgtcctta
    12661 gtttggcagc gtgcgatctg gcgtcgtcac cgtactggca gtttgtgccg ttgcaccctg
    12721 cggcgcagat tgcatcggat gcgcaaggaa ataattgcgc aggcctttgt aaatggcttc
    12781 tgccagctgt tgttggtaat cgtcgctcgc cagcaaacgt tcttcgctgt tgttgctgat
    12841 aaaaccggtt tcgaccagta ctgatgggat atccggcgag cgcagaacgc caaggctggc
    12901 gtgttctggt cgacgtttat gtatttcgcc aatgcgttgc aactgactga tcatactggt
    12961 cgctacatca taccctaccc gctgggaatg accgaactgt aaatccagca ccgcctggct
    13021 taaatagggg tcagactgac tgttcgccag cacatcaccc gccccgccca gcagctccga
    13081 ctgtttctcg tgctgctcca gccagctggc catttcgctg ttggcgcgac ggttagagag
    13141 cacccatacg gaagcgccag tcgcactgcg gttcggtgcg gcatcagcgt gaatcgacac
    13201 gaggaaattg gcgttttgct tacgtgccac atcgctgcgc cccatcaccg agataaagta
    13261 atccccgtca cgggttaaaa cgcctttaaa catcggatcg tcattgagca aagtacgcaa
    13321 tttacgcgcg atggcgatgg tgacattttt ctcccgcgta ccaccggggc cgatagcgcc
    13381 agggtcctga ccgccgtgtc cggcatcaat agcgatgata attttatcgc cattgttagc
    13441 cgtcgcgcgc gctgccggac gcgttaccgt attactgctg ataacacccg tagtgcggtt
    13501 actttccgtt ttaaacggat tgcgcgccgg ttcgctgacg cgcggtgcga caaccgtagg
    13561 cgtttcaacg cgtttcacaa ccacaggcgg cggaggaggc ggtggcggcg catcggcgtt
    13621 aatcgtaaag acgacagtgt aattgctgcc attctgccgc ttcaccgctt cggttttacc
    13681 attttcggta agatcgacca ccagccgtag cgtttgtgca tctttaggcg ttccggagcg
    13741 aatcgccttc accagattat taccgctgaa caacaacggc agtccctgaa tcacgcccgt
    13801 ttgtttgata tcgagcgcca cggtgcgttt gctttgatgg ctaaacgcat aatcaggatc
    13861 gccaataaaa ctcaacgtta tccgcgcctg ttggttgccg ttagaaacct gaatatcaga
    13921 gagcgtcgcg gcacccacct gcgcgcacag cagcagcaac gtcgctacca accaatttct
    13981 gatgcgatac atcatcccac cacctttcaa aggttaaccg gctaaacgtg ccagcaacaa
    14041 ttcacccgca gaggaaaccg cactcacgcg cgcctcacgg ccttgtgcct gataatcaat
    14101 gtgtatttcg acatccgggt caggaagaac acctgtacct tgttgtggcc actccaccag
    14161 gcagatggca tcgttggcaa aataatcgcg gatccccata aactccagct cctcgggatc
    14221 ggcaaggcgg tacaaatcaa agtgataaac cattaaattg tcgagcgtat agggttcgac
    14281 cagcgtataa gtggggcttt tgacattacc ctgatggccc aaagcctgta aaaagccccg
    14341 gctaaaggtg gttttacctg cgcctaaatc gccatacaga tagattacgg ttgcgccatc
    14401 gcaggctttc gctacccgct cgcccaggtc taatgttgcc tgctcatcag ggagcggaat
    14461 tactcgattc atcatggttt ttatcagtca cttccgggtt aacaatacgc tgtagcgtgg
    14521 aaaagagatc ggttgccagc atcccgcgcg ttccaaaacg cgccgccagt acgtcagctg
    14581 ccgcaccgtg cgcgacacag cctgcacagg ctgcatcata cggcgacagt ttttgcccaa
    14641 gcaatgcgcc aataatacca gagagcacat cgcccatgcc gccgcttgcc atgcctgcat
    14701 ttccggcatc aataatgcct aaagcgtcag gatgggcggc gaccacggtt ccggcacctt
    14761 tcagcaccgc tacgccgcca taacgttgta ccagacgttt ggcgcaatgt aagcggtcac
    14821 tttcaatttc agcgacggaa cagcctaaca accgtgcggc ctcgccagga tgcggcgtga
    14881 tcacgcgatt gtgacgctta tcgggattga ttgccagcag gttcaatgca tcggcatccc
    14941 acaacatcgg tttgcgaaaa ttctcaactt tttgcagggc ttttttcccc cactcttgct
    15001 ggcccagacc gggaccaatc accaccacat cggcccattc caggctttcg gtaagagagt
    15061 ccatagtcag ttcatgcacc atcaattctg gtcgtgcagt cagcagcggc gcaatgttct
    15121 cactgcgggt cagtactcgg actaaaccag caccagcacg tagcgccgct tcccccgtca
    15181 tacgaatagc ccccgccgtg ccgtgatcgc caccgataat caccagccgc ccgtgatcgc
    15241 ctttatgcga agtcgggcga cgcggtttca gccagtgaga aagttgttct gccgaaaacc
    15301 gctgaatttt cgtctcctga cctgccagcc agctatccag ccccagtgag tcaaaatgca
    15361 gttgtcctgt aacatcccgc gcttttccag tgagcaagcc tggtttcagc gcaataaaag
    15421 tgatggtgtg atcggcgttg atcactgcgc ctggcgtagc gccggtttca gccagcaggc
    15481 cggaagggat atcaaccgcc acaatcggcg caggatggga attagcgtgg tcgattaact
    15541 ggctaatgga ttcgcggggt gcttgctgca agccggtacc gagcagcgca tcaacaatca
    15601 gagctaccga ctcgggccag acaatattcg aagcatggat ctcgcctccc gcgtttaacc
    15661 atgcctcgcg tgccagcgcg gcctcttccg gcaacggttt atcgctctcc tgggccaaca
    15721 acgtgacctc aatgccgacc gctttggcca gtcgcgcgac cacgtagcca tcgccgccgt
    15781 tattaccatg accgcacaac accagccagt ggcgggcgtc aggatacgcc gaacgacaca
    15841 cctggaatgc ggcctcgcca gcgcgaagca tcagctcata gagtgtgagc cccagcgcat
    15901 ctgccgcctc gcgttctccg cggcggatat cgtcggcgtg ccagacggtg tgtggtatac
    15961 ttacggggtt tttcttcatt gtatggtccg tcatgtcaga gcccctcgat ctcaatcagt
    16021 tagcgcaaaa aattaaacag tgggggctgg aactgggctt tcagcaggta ggtattaccg
    16081 ataccgatct cagcgagtcc gagcccaaac tgcaagcatg gctggacaaa caataccacg
    16141 gcgaaatgga ctggatggca cgtcacggta tgctgcgcgc tcgcccccat gagttattgc
    16201 ccggtacgct gcgcgtgatc agcgtgcgga tgaattacct tcctgctaac gccgcatttg
    16261 ccagcacgct gaaaaacccc aaactcggct atgttagccg ttatgcgctg ggccgtgact
    16321 atcacaaact tctgcgcaac cgactcaaaa agctgggcga gatgattcag caacattgtg
    16381 tttcgctgaa ttttagaccg tttgtcgatt ctgcgcctat tctcgagcgc ccgttagctg
    16441 aaaaagctgg gctcggctgg acaggtaagc actcacttat cctcaatcgc gaggccggtt
    16501 cgttcttctt tttaggcgaa ttgctggtcg atattccgct gcccgtggat caaccagtcg
    16561 aggaaggatg cggcaaatgc gtggcctgta tgacgatttg cccgaccggt gccatcgtcg
    16621 agccatatac cgtcgatgct cgccgctgta tctcttatct caccatcgaa ctggaagggg
    16681 cgatcccgga agagttgcga ccgttaatgg gaaaccgtat ttacggttgc gatgactgcc
    16741 agcttatctg cccgtggaat cgctattcac aactcactac agaagacgat ttcagcccgc
    16801 gtaagccgct acacgcaccg gaactcattg agttattcgc ctggagcgaa gagaagtttt
    16861 taaaagtcac ggaaggttcg gcgattcgcc gtataggtca cctgcgctgg ctgcgtaata
    16921 tcgccgtggc attaggcaat gcgccctggg atgaaacgat tctggcggcg ctcgagagtc
    16981 gcaaaggtga gcacccactt cttgatgagc acatagcgtg ggcgatggcg cagcaaatcg
    17041 agaggcgaaa tgcgtgcata gtcgaagtgc aattgccgaa aaaacagcgt ctggttcggg
    17101 taattgaaaa agggttaccg cgtgacgcct gattcatcca cagcttgtga ataaaaataa
    17161 aaacgcattg caattcaaga cgaataaaat cgtcaagtga tcgactcgac aaaacga
//
LOCUS       DAZFDC010000101        16967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFDC010000101 DAZFDC010000000
VERSION     DAZFDC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.101"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(403..1599)
                     /gene="hemY"
                     /locus_tag="ACHJ48_003454"
     CDS             complement(403..1599)
                     /gene="hemY"
                     /locus_tag="ACHJ48_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO4708938.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1602..2807)
                     /gene="hemX"
                     /locus_tag="ACHJ48_003455"
     CDS             complement(1602..2807)
                     /gene="hemX"
                     /locus_tag="ACHJ48_003455"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO4708939.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKVELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQAD
                     TPAAAPQGE"
     gene            complement(2829..3569)
                     /gene="hemD"
                     /locus_tag="ACHJ48_003456"
     CDS             complement(2829..3569)
                     /gene="hemD"
                     /locus_tag="ACHJ48_003456"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO4708940.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3566..4507)
                     /gene="hemC"
                     /locus_tag="ACHJ48_003457"
     CDS             complement(3566..4507)
                     /gene="hemC"
                     /locus_tag="ACHJ48_003457"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO4708941.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4894..7440
                     /gene="cyaA"
                     /locus_tag="ACHJ48_003458"
     CDS             4894..7440
                     /gene="cyaA"
                     /locus_tag="ACHJ48_003458"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO4708942.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7480..7800)
                     /gene="cyaY"
                     /locus_tag="ACHJ48_003459"
     CDS             complement(7480..7800)
                     /gene="cyaY"
                     /locus_tag="ACHJ48_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO4708943.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFRDLLEQAATQQAGET
                     VSFR"
     gene            complement(7962..8104)
                     /locus_tag="ACHJ48_003460"
                     /pseudo
     CDS             complement(7962..8104)
                     /locus_tag="ACHJ48_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8263..8466
                     /gene="yifL"
                     /locus_tag="ACHJ48_003461"
     CDS             8263..8466
                     /gene="yifL"
                     /locus_tag="ACHJ48_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO4708944.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8503..9327
                     /gene="dapF"
                     /locus_tag="ACHJ48_003462"
     CDS             8503..9327
                     /gene="dapF"
                     /locus_tag="ACHJ48_003462"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO4708945.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9324..10031
                     /gene="yigA"
                     /locus_tag="ACHJ48_003463"
     CDS             9324..10031
                     /gene="yigA"
                     /locus_tag="ACHJ48_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO4708946.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10028..10924
                     /gene="xerC"
                     /locus_tag="ACHJ48_003464"
     CDS             10028..10924
                     /gene="xerC"
                     /locus_tag="ACHJ48_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO4708947.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQNWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKTPRHLPKNIDVDDINRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNALSWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10924..11640
                     /gene="yigB"
                     /locus_tag="ACHJ48_003465"
     CDS             10924..11640
                     /gene="yigB"
                     /locus_tag="ACHJ48_003465"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO4708948.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11724..13886
                     /gene="uvrD"
                     /locus_tag="ACHJ48_003466"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11724..13886
                     /gene="uvrD"
                     /locus_tag="ACHJ48_003466"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO4708949.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13931..14833)
                     /locus_tag="ACHJ48_003467"
     CDS             complement(13931..14833)
                     /locus_tag="ACHJ48_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HFO4708950.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSIV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSRQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(14916..15680)
                     /gene="yigE"
                     /locus_tag="ACHJ48_003468"
     CDS             complement(14916..15680)
                     /gene="yigE"
                     /locus_tag="ACHJ48_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO4708951.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15842..15898
                     /gene="ysgD"
                     /locus_tag="ACHJ48_003469"
     CDS             15842..15898
                     /gene="ysgD"
                     /locus_tag="ACHJ48_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO4708952.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            16050..>16967
                     /gene="corA"
                     /locus_tag="ACHJ48_003470"
     CDS             16050..>16967
                     /gene="corA"
                     /locus_tag="ACHJ48_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO4708953.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAP"
BASE COUNT         3852 a         4461 c         4543 g         4111 t
ORIGIN      
        1 tcattcataa taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat
       61 tactcaggaa gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt
      121 tcccgattct ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct
      181 tagtgcctca taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac
      241 agatgtcgct tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg
      301 ttgtcataca gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgtg
      361 tcaggaaggt cccggaggtg cttgcctccg ggtgagaagg aactactgtg gtgggttgtt
      421 ctgcaacgtt aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag
      481 tctgtccagc gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc
      541 tgcgcggaag gcgagcgatg cttcctgcca ttctccgtgc ttcatcagtg actggcccag
      601 tgtgctccac aacagcgggc gatcgccgac gtttttgatt tgctggcgca gcactttttc
      661 cagctgttcc ggattgtttg ttttcagtcg aggaatcggc agcagtaggc gatcgtcgta
      721 ctggcgtttc aggccatcga tgataatttg ctgggcagta tcatgatcgt cacattcaat
      781 aagatgttcc gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt
      841 tttccaccag ttacgcaaac cttcgctacc gttatcggca cgcgcctgat ccatcaggcc
      901 aatccatgcc tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc
      961 cattgatggg ataatatcca gcagcgaact ccatgcacct gtgcggatat acgcctgttc
     1021 cgccagacgt aatacttccg gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg
     1081 gcgtgcagca tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg
     1141 aatggtgtca ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgcgcttc
     1201 atcaccacgt tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc
     1261 gtgatcggca tttttcgcca tcagcttttc aacttgctga taatcgcctt ccgccagttt
     1321 cagcagcgcc tgttcggtct gcttacgtgc acggcgacgc ttacgcccga caaaccaccc
     1381 acgggtgtgc gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac
     1441 taccatcgcc agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt
     1501 gtcggtctgg atcagcacat aaccctgatg accggcaatc atcgggccaa ccacgatccc
     1561 cgcaatcagc aacacaaaga gcaataacac ttttagcatg gttattctcc ttgcggcgcg
     1621 gctgccggag tatcagcttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt
     1681 ttagcttccg ttgtccccgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc
     1741 agtttttcca gcatcgcctg gctttgcagg gtttccggaa gatccatcga gatattttgc
     1801 tggcttaact ggtctacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag
     1861 taagcacgta cccaggtgga gacgttctcc agcgcctggc gataagtctc ttgctggtga
     1921 cgcggtacag cttgtgctgc gaccagcagg cgagagcgaa tattttcgcg cagatagata
     1981 tcctgatttg gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag
     2041 ttgtccataa agttctgcca gcttttttgc agattgatac gccattcgct gatggaactg
     2101 gaaagctctt caccgtcgga atccatcggc gaaccatcgc tgtcattatc ggccagacgc
     2161 aggttatcta cctgatttga aagctgatta agcttaagga tgatgccgtc ataatccacc
     2221 tgcgatactg cagaaaggct ggcgatatca tcggtaattg cccgacgaac ggtaatcaga
     2281 ctcgggtcat tcatatccgc caggctggcg tctgcacttt tcagcaacgc tgcagcggtc
     2341 gtgacgtcct gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctga
     2401 gccagcagcc aggttttagc atcgctgccg gaaatggtgg cgaccttttg ttggacttca
     2461 tccaactgtt ttgccagcgt ttcttgctga cgattcgcct gcttaagctg tgcagcttgt
     2521 tgcttaataa tgccttccag ctcgactttt tggctctcct gggctttttg caatgccgtc
     2581 agttggttag ccagggcatc gctggtggcg gtctgattga cggcctgttg tttaccccag
     2641 ccatacaaac cgacgcccgc cgccagagca atagcgatag ccaccgcgct gagaatcaat
     2701 gcggtattgt tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc
     2761 tccctggtct cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt
     2821 tatgagagtt attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta
     2881 atgtcttgcc agcccagttc ccgggcgagt ttcgccaaac gctcactgac gaccaatagt
     2941 cgacagtgta gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc
     3001 aacatttcac cgctggtaac aacgaccgtc gtcacctcgc gggattgcca gcgcatcgct
     3061 tcttctgcac catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca
     3121 gcaccgcgcg ccgtcagggt atccccaatt agctcacgac cgccattgcc acgtaatatc
     3181 agcgcacgtt tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt
     3241 tcccgatcct gcgggtagag aatcttctgt ccgcttacgg tatgtagtgc cagtgcggtg
     3301 gtgcgtccaa tggcgaaata attaggtagt cggggccatt tacgatcttg ctgatgtagc
     3361 tgtgattggg caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctccccc
     3421 agcgctgcca gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc
     3481 ggaaaatgcc aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctccagcg
     3541 ggagacgggc gggtgacaag gatactcatg ccggggcgtc tccgttatag acttcagcga
     3601 ggatctcgcg cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg
     3661 catcttgcgg cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga
     3721 ccagcgcacg cagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct
     3781 gacatccacc ttcgagacgg gtattcatgg cgcgttctgc ggtaacgcgc agtgcagttt
     3841 cgtggtgatt cagcgcggca agcagctcgc gagtgcgtgt atcatcaagg cggcattcaa
     3901 taccgactgc accttgtcct accgccggaa gagaaatctc gggtggcaac gcggcgcgaa
     3961 tgcgggactc cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcgtatt
     4021 cgccgttatc cagtttgctc aggcgagtgc cgacgttgcc gcgcagggag cggataatca
     4081 gatccgggcg gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac
     4141 tgcctgccgg taacgcatcc agactgtcat agttattgga cacaaaggca tcgcgaggat
     4201 cttcacgctc acaaatagtg accaggccca gaccttgcgg gaattcaacc ggcacatctt
     4261 tcattgaatg tacggcgata tcggcgcgat tttcgaggag cgcgacttcc agctctttta
     4321 caaataagcc ttttccgccg actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg
     4381 tcaccatcgg taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga
     4441 catagtgtgc ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt
     4501 ctaacatgct tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagtg
     4561 atgtcagtgt tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt
     4621 ggatcctgac aggcgtttca cgccgtcgta ataaggaatt tacagagaat aaacggtgct
     4681 acacttgtat gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg
     4741 ttttagacca ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt
     4801 acctttgaca tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac
     4861 agtcatgacg ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa
     4921 cagagactgg atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct
     4981 gcattccaac aggtctacag tctactgccg acattgttgc actatcacca tccgctaatg
     5041 ccgggttacc ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact
     5101 caacgccact acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggacccgccg
     5161 aaaggtgagc ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa
     5221 agttgttcct ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag
     5281 cgccagttgc tacaacgtaa atgcagcctg ctggaaagct gggccgcctc gctgggtgtg
     5341 gaagttagct tcttcctgat tgatgaaaac cgttttcgcc acaacgagag tggcagtctg
     5401 gggggcgaag attgtggctc cacccagcat atactgctgc ttgatgaatt ttatcgtacc
     5461 gccgtgcgtc tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag
     5521 cattacgatg actacgtaat gacgctctac gcacaaggcg tgctgacgcc aaatgaatgg
     5581 ctggatctcg gcggcttaag ctcgctttcc gctgaagaat acttcggtgc cagcctttgg
     5641 caactctaca agagtatcga ttctccatac aaagcggtgc tgaagacact gctgctggaa
     5701 gcctattcct gggaataccc gaacccacgc ctgctggcga aagatatcaa acagcgtttg
     5761 cacgacggcg agattgtatc gtttggcctc gatccttact gcatgatgct ggagcgtgtt
     5821 actgaatacc tgacggcgat tgaagatttc acccgtctgg atttagtacg tcgctgcttc
     5881 tatttaaaag tgtgcgaaaa gctcagccgt gaacgcgcct gcgtaggctg gcgtcgcgca
     5941 gtgttgagcc agttagtgag cgagtggggg tgggacgaag ctcgtctggc aatgctcgat
     6001 aaccgcgcta actggaagat tgatcaggtg cgtgaggcgc acaacgagtt gctcgacgcg
     6061 atgatgcaga gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc
     6121 gccagtccgc aggatatcgg cgtgctgacg cgtaagctgt atgccgcgtt tgaagcatta
     6181 ccaggtaaag tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg
     6241 acctttattt atgtgccgcc gggccgggct aaccgttcag gttggtatct gtataaccgc
     6301 gcgccaaata ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat
     6361 aaactggtgg cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg cttgtatatt
     6421 aaaggtaacg gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac
     6481 catttcccgt tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatc
     6541 cgccatctgg cgattatcgt taacctggaa tatgacccga cagctgcgtt ccgcaatcag
     6601 gtggtgcatt ttgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc
     6661 ctggtaggta gcgttgacct gctgtatcgc aactcgtgga atgaagtgcg tacgctgcac
     6721 ttcaacggcg agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag
     6781 gacgccgcac cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta
     6841 attcgtactc gcgtgcagca actggtttct gagtgtattg aattgcgtct ttccagcacc
     6901 cgccaggaaa ccgggcgttt caaggcgctg cgcgtttctg gtcaaacctg gggcttgttc
     6961 ttcgaacgcc tgaatgtatc ggtacagaaa ctggagaacg ccatcgagtt ttatggcgct
     7021 atttcgcata acaaactgca cggcctgtca gtgcaggttg aaaccaatca cgtcaaatta
     7081 ccggcggtgg tggacggctt tgccagcgaa gggatcatcc agttcttttt cgaagaaacg
     7141 caagatgaga atggctttaa tatctacatt ctcgacgaaa gcaatcgggt tgaggtgtat
     7201 caccactgcg aaggcagcaa agaggagctg gtacgtgacg tcagtcgctt ctactcgtca
     7261 tcgcatgacc gttttaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat
     7321 cagattgtga aggttgatgg tcgtgaacag gtgattccgt tccgcacaaa atctatcggt
     7381 aacatgccgc ctgccaatca ggatcacgat acgccgctat tacagcagta tttttcgtga
     7441 tgaacgtgcc ggaaagcgag gcttatccgg catgcaatct tagcggaaac tgactgtttc
     7501 acccgcctgc tgcgtcgccg cctgttccag caaatcccgg aaggtttcgc cgctgcgatc
     7561 gcaaatccac tcatcgcctt tcagatcaaa atggtagccg ccttgtttgg ttgccagcca
     7621 tacctggtgc agcggttcct ggcggttgat aatgattttg ctgccattct caaaggtaat
     7681 ggtcagcacg ccgccgttga tttcgcagtc gatatcgctg tcgccatccc agtcgtccag
     7741 acgctcttca atggtcagcc acagttgatc agccaggcga tgaaattcac tgtcgttcat
     7801 tgttgtatcc tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa
     7861 gtttgcacct ctgcggctgc gttacggcat gattcatccg tcaccggaat aatgatgtct
     7921 ctgtgtagcg aaagatttgt ctcttcatta gggcgcagtt acaccacgcc tttccctgtt
     7981 tctggtaaac attatgatca ggtttaccga gcgagcatcc tcacgctgac ggaactaaaa
     8041 aagccaacaa acaaaccaca ttgcgatagt gcataaagcc atcctggcgt gaggtgttga
     8101 tcacgaaact gccagcagaa cataaatcct cacgagtaag cgttatactc gcagcatttc
     8161 ctcacttttc agacttcata aagagtcgct aaacgcttgc ttttacgcct tctcctgcga
     8221 tgatagaaag cagaaagcga tgaactttac aggcaatcca taatgaaaaa cgtgtttaag
     8281 gcactcactg tattacttac tctcttcagc ctgacgggct gcggtctgaa aggtccgctc
     8341 tatttcccgc ctgcagataa aaatgcaccg ccgccgacca aaccggtaga gacgcaaacg
     8401 caatccacgg tgccggataa aaacgatcgc gccactggcg atggtccatc ccaggtgaat
     8461 tactaaaagt cagtttctgt acccgcgtga ttggagtaaa tgatgcagtt ctcgaaaatg
     8521 catggccttg gcaacgattt tatggtcgtc gacgcggtaa cgcagaatgt ctttttttca
     8581 ccggagctga ttcgtcgcct agctgatcgg cacctggggg tagggtttga ccaactgctg
     8641 gtggttgagc cgccgtatga tcctgaactg gattttcact atcgcatttt caatgctgat
     8701 ggcagtgaag tggcgcagtg cggcaacggc gcacgctgct ttgcccgttt tgtgcgtctg
     8761 aaagggctga ccaataagcg cgatatccgc gtcagcaccg ccaacgggcg gatggttctt
     8821 accgtcaccg atgatgatct ggtccgcgta aatatgggcg aacccaactt cgaaccttct
     8881 gccgtgccgt ttcgcgctaa caaagcggaa aagacctata ttatgcgcgc cgccgagcag
     8941 acaatcttat gcggcgtggt gtcgatggga aatccacatt gcgtgattca ggtcgatgat
     9001 gtcgatactg cggcggtaga aacactgggg cctgttctgg aaagccacga gcgttttccg
     9061 gagcgcgcca atatcggttt tatgcaagtg gttaagcgcg agcatattcg tttacgcgtt
     9121 tatgagcgtg gggcaggaga aacccaggcc tgcggcagcg gcgcatgtgc ggcggttgca
     9181 gtagggattc agcaaggttt gctggccgaa gaagtacgcg tggaactccc aggcggtcgt
     9241 cttgatatcg cctggaaagg tccgggtcac ccgttatata tgactggccc ggcggtacat
     9301 gtctacgacg gatttattca tctatgaagc aaccagggga agaactgcag gaaacactca
     9361 cggagcttga tgaccgggtg gttgtcgatt atctgattaa aaatcctgag ttttttatcc
     9421 gcaatgcgcg cgcagtagaa gcgatacgtg tgccgcatcc ggtacgcggt accgtttcgt
     9481 tggtcgagtg gcacatggcc cgcgcacgta atcatattca tgttctggaa gagaacatgg
     9541 cgctgttgat ggaacaggct atcgccaacg aaggcctgtt ttatcgctta ctctacctgc
     9601 agcgcagtct caccgccgcc agcagtcttg atgatatgct gatgcgcttt caccgctggg
     9661 cgcgtgatct cggcctggca ggtgcgagtc tgcgcctgtt tccggatcgc tggcgcttag
     9721 gtgcgccgtc gaaccacact catctggcat taagccgtca gtctttcgaa ccgctgcgta
     9781 ttcagcgtct ggggcaggag cagcattatc tggggccgct taacggaccg gagctgctgg
     9841 tggtactacc ggaagcgaaa gcggtgggat cggtggcgat gtcgatgctg ggaagcgatg
     9901 ctgatttagg cgtggtgctg ttcaccagcc gcgatgccag tcactatcaa caagggcagg
     9961 gaacgcagtt acttcatgaa attgcgctga tgttgccgga gcttctggag cgttggattg
    10021 aacgcgtatg accgatttac acaccgatgt agaacgctac ctacgttatc tgagcgtgga
    10081 gcggcagctt agtccgataa ctctgcttaa ctaccagcga cagcttgagg cgatcatcaa
    10141 ttttgccagc gaaaacggcc tgcaaaactg gcagcaatgc gatgcagcga tggtgcgcaa
    10201 ttttgctgta cgcagtcgcc gtaaagggct gggcgcggca agcctcgcat tacgcctctc
    10261 tgcgctacgt agcttttttg actggctggt cagccagaac gaactcaaag ctaacccggc
    10321 gaaaggtgtt tcggcaccga aaacgccacg tcatctgccg aaaaatatcg acgtcgatga
    10381 tataaatcgc ctgttggata ttgatatcaa cgatcccctc gctgtgcgcg accgtgcaat
    10441 gctggaagtg atgtacggcg cgggcctgcg tctttccgag ctggtggggc tggacatcaa
    10501 acacctcgac ctggagtccg gcgaagtgtg ggtgatgggg aaaggcagca aagagcgccg
    10561 cctgccgatt ggtcgtaacg ctttgtcatg gattgagcac tggcttgatt tgcgcgacct
    10621 gtttggtagc gaagacgacg cgctttttct gtcgaaactg ggcaagcgta tctccgcgcg
    10681 taatgtgcag aaacgctttg ccgaatgggg cataaaacaa gggctgaata atcacgttca
    10741 cccgcataaa ttacgtcact cgttcgctac gcatatgctg gagtcgagcg gcgatcttcg
    10801 tggtgtgcag gagctgctgg gtcatgccaa cctctccacc acgcaaatct atactcatct
    10861 tgattttcaa caccttgcct cggtgtacga tgcggcgcat ccacgcgcca aacgggggaa
    10921 ataatgcgtt tttaccggcc tttggggcgc atctcggcgc tcacctttga cctggatgat
    10981 accctttacg ataaccgtcc ggtgattttg cgcaccgagc aggaggcgct tacctttgtg
    11041 caaaattacc atccggcgct gcgcagcttc cagaatgaag atctgcaacg cctgcgccag
    11101 gcggtacggg aagcggaacc cgagatttat cacgacgtga cgcgctggcg ttttcgctca
    11161 atagaacaag cgatgcttga cgccggattg agcgcggaag aagccagtgc aggcgcacac
    11221 gcggcaatga tcaactttgc taaatggcgc agcaggatcg acgtcccgca gcaaacccac
    11281 gacaccttaa aacagctggc gaagaaatgg ccgctggtgg cgatcaccaa cggtaacgct
    11341 cagccggaac tgtttggttt gggggattat tttgagtttg tgctgcgcgc tggcccgcac
    11401 gggcgctcga aaccgttcag cgatatgtac ttcctggcgg cggaaaaact caacgtgccg
    11461 atcggcgaga tcttacatgt aggtgacgat ctcaccactg acgtgggtgg ggcaattcgc
    11521 agcggaatgc aggcctgttg gatcagaccg gaaaatggcg atctgatgca aacctgggat
    11581 agccgtttac tcccgcatct ggaaatttcc cggttggcat ctctgacctc gctgatataa
    11641 tcagcaaatc tgtatatata cccagctttt tggcggaggg cgttgcgctt ctccgcccaa
    11701 cctattttta cgcggcggtg ccaatggacg tttcttacct gctcgacagc cttaatgaca
    11761 aacagcgcga agcggtggcc gcgccgcgca gcaaccttct ggtgctggcg ggcgcgggca
    11821 gtggtaagac gcgcgtactg gtgcatcgta tcgcctggct gatgagcgtg gaaaactgct
    11881 cgccatactc gattatggct gtgacgttta ccaacaaagc ggcggcggag atgcgtcatc
    11941 gtatcgggca actgatgggc accagccagg gcggcatgtg ggtcggcacc ttccacgggc
    12001 tggcgcaccg tttgctgcgt gcgcaccata tggacgccaa tctgccgcag gatttccaga
    12061 tcctcgatag cgaagaccag ctacgcctgc ttaagcgtct gatcaaagcc atgaacctcg
    12121 acgagaagca gtggccgccg cgccaggcaa tgtggtacat caacagccag aaagatgaag
    12181 gcctgcgtcc gcatcatatt caaagctacg gtaatccggt ggagcagacc tggcagaagg
    12241 tgtatcaggc gtatcaggaa gcgtgcgatc gtgcaggcct ggtggacttc gccgagctgc
    12301 tgctgcgcgc tcacgagttg tggcttaaca agccgcatat cctgcaacac taccgcgaac
    12361 gttttaccaa catcctggtg gacgaattcc aggataccaa caacattcag tacgcgtgga
    12421 tccgcctgct ggcgggcgac accggcaaag tgatgatcgt cggtgatgac gaccagtcaa
    12481 tctacggctg gcgcggggcg caggtggaga atattcagcg tttcctcaat gatttccccg
    12541 gtgccgaaac tatccgtctg gagcagaact atcgttctac cagtaacatt ctgagcgccg
    12601 cgaacgccct gattgaaaac aacaacgggc gtctgggtaa aaaactgtgg accgatggcg
    12661 cggacggtga gcctatttcc ctctattgcg cttttaacga actcgatgaa gcgcgttttg
    12721 tcgttaaccg catcaaaacc tggcaggaca acggtggggc gctcgccgag tgcgccattc
    12781 tctaccgcag caacgcccag tcgcgggtgc tcgaagaggc gttattgcag gccagtatgc
    12841 cgtaccgtat ttacggcggg atgcgcttct tcgaacgcca ggaaatcaaa gatgcgctct
    12901 cgtatctgcg cctgattgcc aaccgcaacg acgacgcggc ctttgagcgt gtggtgaata
    12961 cgccaacgcg gggtattggt gaccggacgc tggacgtggt acgtcagaca tcgcgcgatc
    13021 gccagttaac actctggcag gcatgtcgtg aactgttgca ggaaaaagcc ctcgccggac
    13081 gtgctgccag cgccttacag cggtttatgg aactgatcga cgccttagcg caggaaactg
    13141 ccgatatgcc gctgcatgta cagactgacc gggtaattaa agactccggc ctgcgtacca
    13201 tgtacgagca ggagaagggc gaaaaaggtc agacgcgtat cgaaaactta gaggaactag
    13261 tgacggcaac gcgccaattc agctacaacg aagaagacga agatttaatg ccgctgcagg
    13321 cgttcctctc ccatgcggcg ctggaagcgg gcgaggggca ggcggatacc tggcaggatg
    13381 cagtgcagtt gatgacgctg cactcggcga aaggcctgga gttcccgcag gtgtttatcg
    13441 tcggtatgga agagggcatg ttcccaagcc agatgtcgct ggatgaaggc ggacgtctgg
    13501 aagaagaacg ccgtctggcc tacgttggtg taacccgcgc gatgcagaaa ctgacgctga
    13561 cctacgcgga aactcgccgt ctgtatggca aagaggttta ccatcgcccg tcgcgcttta
    13621 tcggtgagtt gccggaagag tgtgtggaag aggtgcgcct gcgcgccacg gtaagccgcc
    13681 cggtcagcca tcagcgtatg ggtacgccga tggtcgagaa cgacagcggc tacaagctcg
    13741 gccagcgtgt acgccacgct aagtttggtg aaggcactat cgtcaatatg gaaggcagcg
    13801 gcgagcatag ccgtttgcag gtggcattcc agggccaggg tattaaatgg ctggtggcgg
    13861 catacgcccg gctggagacg gtataacgtt gcgggatgcg gtgcgccaca ccgcatccgg
    13921 catcagcacc ttacttatca catttcttca catcaatatt ccagtcagca ccagacgcaa
    13981 cctgatgttt ctgcgcgaaa ttatccatat tcagcgccac ggaaacattc aataagctgt
    14041 tcagatacac catcggctgt ccttccggca catcgccgaa gcttgcgaca tacggcattt
    14101 tccctgcgta tttttgccgg gaaccttcgg aaatggtcac acacaacgtg tcgttgagtt
    14161 tgatccccgc ctgattcagc aactcgtcgc tgatattgct ccagacgttg ccgtactgaa
    14221 tatcgagaat cgggatattg cccttcactt caccttttgt tgcttttgct ttctgataag
    14281 aaagttcgac aacctttgca ggaagctcag gccccacctg ctcgaacgtg atcgccccgg
    14341 aagccagacg tgcaccggtg taggcataca cgtcacgacc gtggaaggta tatgactttt
    14401 cagaaccttt caggcggtta gctttttcgt cgatttcccg gacgctctca atacccagcg
    14461 attccgccac caaagtgagc gtaccgttat ccggtgaaac gaagtattga ccgtttttgg
    14521 ttttcagcac tatcgattta cggtcagtgc ctacgcccgg atcgaccaca ctgacaaata
    14581 ccgaaccttg cggccagtaa ctggcggtct gatacaggcg gtaagcgcct tcccagatgt
    14641 tatacggtgg aatttcgtgt gttaaatcaa atattttaag attatggtca acaccgaatg
    14701 cgaccccttt catcgccgag acggcaccat ctttcaggct aaaatcagtt tgtaaaatca
    14761 aagcgttatc cgcataagcg gatgatgatc ccagacacgt taataaaaat aacgttgcga
    14821 gcgttttttt catttttgtt tcccttcatt tatctgatga ttttccagct gtgtagccag
    14881 ccccagtttg tctggcctcc tcgagtgtac aatgcttaac ctttccgctc gacggaaatc
    14941 atggtgacaa aaggatagcg ttgccacggg attgccccgc ctttcatata catatgtgaa
    15001 atcgtgccat caagataaag cagctgctca acgttcagtt tcgctttggc ataacaggca
    15061 aaatcataaa aatttgtcgc ctgctggctc aacaaaaaca cggcattccc atgtttatta
    15121 attccaacac cgttacgaat tttacgtgag gcgacgttgg gatgaatacg cggattaatt
    15181 acactgtttt ccatcaacat tggccctgac tgcaccgcaa actgaatctc tttactggtt
    15241 ttgaaggcat ccagacgaac gatgccgact ttatctcccg cgacataaaa cacgccgcca
    15301 ggacggataa agaaattccc ttcacctgaa gcgagattta acgccacctt ctgctgaccg
    15361 ttttcgatgt acaaaccgag cggcgcatag ctttcatcat agatgccgcc gttcatcgcc
    15421 atctgcacct gaccctgact attcatatcc gccagcagag catgtaaagt tccccacgct
    15481 tcgccattgg ctttttgcca gtacattttc acccgctctg tttgaggatt aacggtatac
    15541 gcctgtacgg tcagcgttgg atctgagagt gcgcaatcat cagcggcaac agcaaacaag
    15601 ggaagtaagg tgagggcgag aaaaatccgt ttgagattca aggtgatcat tcctttacca
    15661 atgagtagcc gatgcgccat tataggtcct ggatgtggga tttttttatc ctgttagcga
    15721 ccttgacgag taccaaaaag cgcgaagttc aactattgtt ctgtggtgtt ctgttgcgtg
    15781 ttgacggcaa aattttgctg gcgtaacatg cgcgcacgat cactctaaga ggacattcgc
    15841 cttggacaca cccagtagat actggctcac tatcctgtca tccaggatca actcctaagg
    15901 ctatcccttt ttgctgatag ccttagcggt tgtcagcgac ctcaattttt cccgtcgcgc
    15961 tgagtcaggc tgtttaatgg tctgaaaccc aatttgtttc tgtgtgccca ccgaactgtc
    16021 cgatatttta agcattggga gtcccggtca tgctgagcgc atttcaactg gaaaataacc
    16081 gactgacccg gctggaagtc gaagagtcac aaccccttgt aaatgcagta tggattgatc
    16141 ttgtcgaacc ggacgacgac gagcgactgc gcgtacaatc tgaacttggc cagagcctgg
    16201 caacccgccc ggaactggaa gacatcgaag catcggcacg tttctttgaa gacgatgacg
    16261 gcctgcatat tcactccttc ttcttctttg aagatgcgga agatcacgcc ggtaactcca
    16321 ctgtggcatt taccatccgt gatggtcgtc tgtttactct gcgtgagcgt gaactgcccg
    16381 cttttcgtct gtatcgtatg cgtgcccgta gccagtcgat ggtagacggt aacgcctacg
    16441 agttgctgct ggatctgttc gaaaccaaaa tcgaacagtt ggcagatgaa attgaaaata
    16501 tctatagcga cctggagcaa ttgagccggg tgattatgga agggcatcag ggcgatgagt
    16561 acgacgaggc gctctccact ctggcggaac tggaagatat cggctggaaa gtgcgcctgt
    16621 gtctgatgga tacccagcgc gcgctcaact tcctggtacg taaagcacgc ttaccgggtg
    16681 ggcaactgga gcaggcgcgt gaaatcctgc gagatatcga atccctgctg ccgcataacg
    16741 aatccctgtt ccagaaggtg aacttcctga tgcaggcggc aatgggtttt atcaacatcg
    16801 agcagaaccg catcatcaaa attttctcgg tggtatccgt cgtgttcctg ccgccaacgc
    16861 tggttgcttc cagctacggg atgaactttg agtttatgcc agaactgaag tggagtttcg
    16921 gctacccagg cgcgattatc tttatgatcc tcgcgggcct ggcaccg
//
LOCUS       DAZFDC010000102        16834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFDC010000102 DAZFDC010000000
VERSION     DAZFDC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.102"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            75..2771
                     /gene="stcE"
                     /locus_tag="ACHJ48_003471"
     CDS             75..2771
                     /gene="stcE"
                     /locus_tag="ACHJ48_003471"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFO4708954.1"
                     /translation="MNTKMNERWRTPMKLKYLSCTILAPLAIGVFSATAADNNSAIYF
                     NTSQPINDLQGSLAAEVKFAQSQILPAHPKEGDSQPHLTSLRKSLLLVRPVKADDKTP
                     VQVEARDDNNKILGTLTLYPPSSLPDTIYHLDGVPEGGIDFTPHNGTKKIINTVAEVN
                     KLSDASGSSIHSHLTNNALVEIHTANGRWVRDIYLPQGPDLEGKMVRFVSSAGYSSTV
                     FYGDRKVTLSVGNTLLFKYVNGQWFRSGELENNRITYAQHIWSAELPAHWIVPGLNLV
                     IKQGNLSGRLNDIKIGAPGELLLHTIDIGMLTTPRDRFDFAKDKEAHREYFQTIPVSR
                     MIVNNYAPLHLKEVMLPTGELLTDMDPGNGGWHSGTMRQRIGKELVSHGIDNANYGLN
                     STAGLGENSHPYVVAQLAAHNSRGNYANGIQVHGGSGGGGIVTLDSTLGNEFSHEVGH
                     NYGLGHYVDGFKGSVHRSAENNNSTWGWDGDKKRFIPNFYPSQTNEKSCLNNQCQEPF
                     DGHKFGFDAMAGGSPFSAANRFTMYTPNSSAIIQRFFENKAVFDSRSSTGFSKWNADT
                     QEMEPYEHTIDRAEQITASVNELSESKMAELMAEYAVVKVHMWNGNWTRNIYIPTASA
                     DNRGSILTINHEAGYNSYLFINGDEKVVSQGYKKSFVSDGQFWKERDVVDTREARKPE
                     QFGVPVTTLVGYYDPEGTLSSYIYPAMYGAYGFTYSDDSQNLSDNDCQLQVDTKEGQL
                     RFRLANHRANNTVMNKFHINVPTESQPTQATLVCNNKILDTKSLTPAPEGLTYTVNGQ
                     ALPAKENEGCIVSVNSGKRYCLPVGQRSGYSLPDWIVGQEVYVDSGAKAKVLLSDWDN
                     LSYNRIGEFVGNVNPADMKKVKAWNGQYLDFSKPRSMRVVYK"
     gene            2858..3733
                     /gene="gspC"
                     /locus_tag="ACHJ48_003472"
     CDS             2858..3733
                     /gene="gspC"
                     /locus_tag="ACHJ48_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO4708955.1"
                     /translation="MLFFLSFRGDRGLFIKDIVLKMLTPNRLLCVILLIAGYQLVSVI
                     HHFWLTQAASVPGLSRVSAPETAVTGDQTEERFVFTLFGRASPLSSEGRAQETMPSLS
                     DDLLSGEDLDVRGILYSSVAEHSVAIFAHNNRQFSLSVGEKVPSYDATISAIFSDHIV
                     INYQGKTVSLPLRYDNTEKKNAYDNNNLTVGDVITQDNFRVESVFDIMSFSAVTVNNT
                     LSGYRLIPGKHSSLFYNAGLHDNDLAVSVNGSELRDTRQAQQIMKQLPELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3734..5701
                     /gene="etpD"
                     /locus_tag="ACHJ48_003473"
     CDS             3734..5701
                     /gene="etpD"
                     /locus_tag="ACHJ48_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFO4708956.1"
                     /translation="MFRKWLNGRLPVLVFTTVILGAIPGWGAEFSANFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDVSPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKVGNRSVATVPLTYASATDVARLVTELTKET
                     DKTAIPAWMTAKLVADERTNSVLVSGEPISQQRIISIIKQLDRQEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSSIENDSKKSPSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEIQDADGLNLGIQWVNKHAGVAQFTSTGLPITTMVQTR
                     QNEILDSDQSNALSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFRAKSEQTAKRNLMLFIRPTIIRERDGFRHASAEKYQSFNQEQVQSRGKETTALTL
                     NEEQLRLSPDQDDTAFRKVKAAIAAFYAQEM"
     gene            5701..7206
                     /gene="gspE"
                     /locus_tag="ACHJ48_003474"
     CDS             5701..7206
                     /gene="gspE"
                     /locus_tag="ACHJ48_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO4708957.1"
                     /translation="MSRVVQNVSESRPLLPFSFSRTQRILLLREQEGNRVFCMEDTPA
                     SALLEVRRVAEGPLNVTTVSAEAFEKQLVSSYQRDSDEARQMMAEIGNEMDFYTVAGE
                     LPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRIDGVLREIL
                     RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIV
                     LRLLDKNSVSLDLAALGMSQQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLL
                     NPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDGE
                     TAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLC
                     PRCRQPCQVSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTA
                     IYQGEGELGITRLAGSRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7208..8431
                     /gene="gspF"
                     /locus_tag="ACHJ48_003475"
     CDS             7208..8431
                     /gene="gspF"
                     /locus_tag="ACHJ48_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO4708958.1"
                     /translation="MALFHYQASDIHGRKRSGILEADSARHARQLLREQALIPVRLDE
                     KQVHHKHSLRSILRFRPRGGSSAELALLTRQLATLVAASLPLEEALDALLRQSEKPRQ
                     RNLIAAVRTKVLEGHSLAAAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALLYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGA
                     AEVSQTWGPWLLLAAALGGIAGRMILHQPSQRLAFHHLLLRLPVVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMHISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPP
                     MMRHMIASGENSGELDSMLERAADNQDREFSTQMQLALGLFEPLLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNM"
     gene            8462..8896
                     /gene="gspG"
                     /locus_tag="ACHJ48_003476"
     CDS             8462..8896
                     /gene="gspG"
                     /locus_tag="ACHJ48_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO4708959.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     MSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP"
     gene            8893..9447
                     /gene="gspH"
                     /locus_tag="ACHJ48_003477"
     CDS             8893..9447
                     /gene="gspH"
                     /locus_tag="ACHJ48_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO4708960.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSQQDGLLMGIQVRFDGWKFKVLQRGTAESPPTLAEGGDIWQGYVWQTW
                     QPRRAAMGGKLPDDVRLELQYLRGLQWMSEHDDGAEPDILLLPGGEVTPFRLLFRQVG
                     EEAVVGLQVDENGLMTLFEGEVSL"
     gene            9444..9809
                     /gene="gspI"
                     /locus_tag="ACHJ48_003478"
     CDS             9444..9809
                     /gene="gspI"
                     /locus_tag="ACHJ48_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO4708961.1"
                     /translation="MKGQEGMTLLEVIVALVVFALAGMALMQASTQQAAGIGRMEEKV
                     LAGWLADNQMVQLQLEKTWPENGWGEKTISFAGTEWYLRWGGPDSDVPQPRSLEVEVR
                     RTKEETSALVSLRSSVVRE"
     gene            9806..10405
                     /gene="gspJ"
                     /locus_tag="ACHJ48_003479"
     CDS             9806..10405
                     /gene="gspJ"
                     /locus_tag="ACHJ48_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO4708962.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQGGIRAHELSRD
                     KVQRLAELQRGISQMERDLTQMLPRHSRGNEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFHVDHPSGVSPDVRVMLDGVHAFRLRFFVNGD
                     WQARWDSTGILPQAVEVTLVMDDFAELPRLFLVSKETAE"
     gene            10402..11379
                     /gene="gspK"
                     /locus_tag="ACHJ48_003480"
     CDS             10402..11379
                     /gene="gspK"
                     /locus_tag="ACHJ48_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO4708963.1"
                     /translation="MKLREQGVALLVVLLILSLMVTIAAVIAERNGRTFLRTVAQLDQ
                     LQAKWDGYTAETIAKQILQRSRQESPRKTHLAQNWAQSERQFETRGGDVRGQIVDAQA
                     CFNLNAINYGVVDLTSIPYAARIFQQLLINLQVELLQARQVTAALRDWIDRDDKPVRG
                     GAEDEVYMGMEPPYLAANQPMQDVSELRLIRGIDARLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPDSLPVTELLQRRPRTGWESVAAFLDAPPLKDIDTSAAMPV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRIIQRQYGLTMREVP"
     gene            11313..12590
                     /gene="gspL"
                     /locus_tag="ACHJ48_003481"
     CDS             11313..12590
                     /gene="gspL"
                     /locus_tag="ACHJ48_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO4708964.1"
                     /translation="MEGGAFPDHSATVWINDEGGSLKKNNKHVEAGRLFLRLPEDDSE
                     PLLWRLIAPEETGAGSVAVGETDPQLVGLLSRYPAWVLVPSGRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQSGDRCDVAVVEKAVMHRWLAYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVKLAGQWLFRRDRYAGMTVELSWLTHFLSLTAPPVIESYSV
                     PPVPCPGPKTTEWRNQPGRDLLQLVAEGDGYDGADLRQGEFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAQFWRYESVRVYQQLFPSEKAVKDPRRQMLRHLQ
                     QSTGNNIPELGSVMRQLQQLLSETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     IAGEKYLIQPGEIRQQPQGVESRLILRVNNERA"
     gene            12577..13089
                     /gene="gspM"
                     /locus_tag="ACHJ48_003482"
     CDS             12577..13089
                     /gene="gspM"
                     /locus_tag="ACHJ48_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO4708965.1"
                     /translation="MNELKKRLQYVSPRERRLLIGCVALLVVVFVYYVLWQPWLIRED
                     KWRTVVTREKNTVEWMKQQVPNIKRREASMPEQGEPLGLSAAVTRTSAAYGVSVTRLQ
                     PQGERLAVTLAPVEFNALMQWLTQLERRHRIRTMVFDVAAQGNPPGQVTVNRLVLSQD
                     DVNKDASLSR"
     gene            13210..13980
                     /locus_tag="ACHJ48_003483"
     CDS             13210..13980
                     /locus_tag="ACHJ48_003483"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO4708966.1"
                     /translation="MGGVTGSFLNVVIHRLPIMLERRWRREALTLLAHSVPESGPAFN
                     LMIPQSHCPCCFHPLGIRDNIPLLSYLFLKGKSRCCGERIPVYYPLVEITNSVLFILA
                     ASRFPPGLTLAAAWLFISMLLVLAVIDCHTALLPDVLTLPLLWLGLLFSLQRGVVTLE
                     EAVVGAVSGYLCLWGLYWLFRFATGREGLGYGDFKLAAALGAWMGWQALPSILLFASV
                     SGLIVTVLLRIVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            14072..14473
                     /gene="eptO"
                     /locus_tag="ACHJ48_003484"
                     /gene_synonym="gspS"
     CDS             14072..14473
                     /gene="eptO"
                     /locus_tag="ACHJ48_003484"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFO4708967.1"
                     /translation="MMGNILKKLNCIASLLVLVTISGCHQSPSIHKQATVPPSEQLEQ
                     MASIVSATRYLKMRCNRSDLPDEQSILNVANRIAIGKGWQSLTQEDIRKHSDDIYVRL
                     TRDSTPEYIKCREFNRRLVPFIGELLARGRG"
     gene            14652..15823
                     /locus_tag="ACHJ48_003485"
     CDS             join(14652..14901,14901..15823)
                     /locus_tag="ACHJ48_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611522.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4708968.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDIGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYNRALDVRLPEQFSI
                     IGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHNISHGSAGA
                     RSIATMATLRGFRMGRWLAGRLMKELGLVSCQQPAHRYKRGGREHVTIPNHLGRQFAV
                     TEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTIKALKMAWEI
                     RSKPAGVMFHSDQGSHYTSRQFRQLLWRYQIKQSLSRRGNCWDNSPMERFFRSLKNEW
                     IPVTGYMNFSDAAHEITDYIVGYYNALRPHEYNGGLPPNESENRYWKNSKAVASFC"
     gene            16364..>16834
                     /gene="ehxC"
                     /locus_tag="ACHJ48_003486"
                     /gene_synonym="hlyC"
     CDS             16364..>16834
                     /gene="ehxC"
                     /locus_tag="ACHJ48_003486"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO4708969.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEV"
BASE COUNT         4196 a         3662 c         4737 g         4239 t
ORIGIN      
        1 aaaaataacc aacgactagt gaataagtcg ctcctgaaaa aataaaatat agaaatactg
       61 ttatatccgg ctgcatgaac actaaaatga atgagagatg gagaacaccg atgaaattaa
      121 agtatctgtc atgtacgatc cttgcccctc tggcgattgg ggtattttct gcaacagctg
      181 ctgataataa ttcagccatt tatttcaata cctcccagcc tataaatgat ctgcagggtt
      241 cgttggccgc agaggtgaaa tttgcacaaa gccagatttt acccgcccat cctaaagaag
      301 gggatagtca accacatctg accagcctgc ggaaaagtct gctgcttgtc cgtccggtga
      361 aagctgatga taaaacacct gttcaggtgg aagcccgcga tgataataat aaaattctcg
      421 gtacgttaac cctttatcct ccttcatcac taccggatac aatctaccat ctggatggtg
      481 ttccggaagg tggtatcgat ttcacacctc ataatggaac gaaaaagatc attaatacgg
      541 tggctgaagt aaacaaactc agtgatgcca gcgggagttc tattcatagc catctaacaa
      601 ataatgcact ggtggagatc catactgcaa atggtcgttg ggtaagagac atttatctgc
      661 cgcagggacc cgaccttgaa ggtaagatgg ttcgctttgt ttcgtctgca ggctatagtt
      721 caacggtttt ttatggtgat cgaaaagtca cactctcggt gggtaacact cttctgttca
      781 aatatgtaaa tggtcagtgg ttccgctccg gtgaactgga gaataatcga atcacttatg
      841 ctcagcatat ttggagtgct gaactgcctg cgcactggat cgtgcctggt ttaaacttgg
      901 tgattaaaca gggcaatctg agcggtcgcc taaatgatat caagattgga gcaccgggtg
      961 agctgttgtt gcatacaatt gatatcggga tgttgaccac tccccgggat cgctttgatt
     1021 ttgccaaaga caaagaagca catagggaat atttccagac cattcctgta agtcgtatga
     1081 ttgttaataa ttatgcgcct ctacacctaa aggaagttat gttaccaacc ggagagttat
     1141 tgacagatat ggatccagga aatggtgggt ggcatagtgg tacaatgcgt caaagaatag
     1201 gtaaagaatt ggtttcgcat ggcattgata atgctaacta tggtttaaat agtaccgcag
     1261 gcttagggga gaatagtcat ccatatgtag ttgcgcaatt agcggcacat aatagccgcg
     1321 gtaattatgc taatggcatc caggttcatg gtggctccgg aggtggggga attgttactt
     1381 tagattccac attggggaat gagttcagtc atgaagttgg tcataattat ggtcttggtc
     1441 attatgtaga tggtttcaag ggttctgtac atcgtagtgc agaaaataac aactcaactt
     1501 ggggatggga tggtgataaa aaacggttta ttcctaactt ttatccgtct caaacaaatg
     1561 aaaagagttg tctgaataat cagtgtcaag aaccgtttga tggacacaaa tttggttttg
     1621 acgccatggc gggaggcagc cctttctctg ctgcaaaccg tttcacaatg tatactccga
     1681 attcatcggc tatcatccag cgtttttttg aaaataaagc tgtgttcgat agccgttcct
     1741 ccaccggctt cagcaagtgg aatgcagata cgcaggaaat ggaaccgtat gaacacacca
     1801 ttgaccgtgc ggagcagatt acggcttcag tcaatgagct aagtgaaagc aaaatggctg
     1861 agctgatggc agagtacgct gtcgtcaaag tgcatatgtg gaacggtaac tggacaagaa
     1921 acatctatat ccctacagcc tccgcagata atagaggcag tatcctgacc atcaaccatg
     1981 aggccggtta taatagttat ctgtttataa atggtgacga aaaggtcgtt tcccaggggt
     2041 ataaaaagag ctttgtttcc gatggtcagt tctggaaaga acgtgatgtg gttgatactc
     2101 gtgaagcgcg taagccagag cagtttggtg ttcctgtgac gaccctggtg gggtattacg
     2161 atccggaagg cacgctgtca agctacatct atcctgcgat gtatggtgcc tatggcttca
     2221 cttattccga tgatagtcag aatctatccg ataacgactg ccagctgcag gtggatacga
     2281 aagaagggca gttgcgattc agactggcta atcaccgggc taacaacact gtaatgaata
     2341 agttccatat taacgtgcca acagaaagtc agcccacaca ggccacattg gtttgcaata
     2401 acaagatact ggataccaaa tcgctcacac ctgcgccaga aggacttacc tatactgtaa
     2461 atgggcaggc acttccagca aaagaaaacg agggatgcat cgtgtccgtg aattcaggta
     2521 aacgttactg tttgccggtt ggtcaacggt caggatatag ccttcctgac tggattgttg
     2581 ggcaggaagt ctatgtcgac agcggggcta aagcgaaagt gctgctttct gactgggata
     2641 acctgtccta taacaggatt ggtgagtttg taggtaatgt gaacccagct gatatgaaaa
     2701 aagttaaagc ctggaacgga cagtatttgg acttcagtaa acctaggtca atgagggttg
     2761 tatataaata atcctgagac ggtgcggcca cttcggccgc attagtaaca cattcgtccc
     2821 gttaatcaaa acataaagta gcgtccagcg ttttattatg ttgttctttc tatcattccg
     2881 gggtgaccgg ggcttattta taaaagatat cgtacttaaa atgctgacgc caaaccggct
     2941 attgtgcgta attttactta ttgccggata tcagctggtg tcggttatcc atcatttctg
     3001 gctgactcag gcagcatcgg tgcccggctt atcccgtgtt agcgcgccgg aaacagcggt
     3061 aactggtgat caaactgaag aacgttttgt ttttacgtta ttcggcaggg catccccact
     3121 atcatcggag gggagagcgc aggaaacaat gccttccctg tcagatgatc tgctttcagg
     3181 ggaggatctt gacgtgagag gtatacttta tagttcggtt gcagagcatt ccgttgccat
     3241 atttgcacat aataacagac agttcagtct gagcgtcggc gaaaaagtac ccagctacga
     3301 tgctacgatt agtgctattt ttagcgatca tattgttatt aactatcagg gaaaaactgt
     3361 atcactgcct ctgcgatatg ataataccga aaaaaagaat gcatatgaca ataataattt
     3421 aacagttgga gacgtgataa cccaagataa ttttcgggta gaaagtgttt ttgatattat
     3481 gagcttttca gccgttacgg ttaataatac attaagcggt tatcgcttga ttccgggtaa
     3541 gcacagttcg ttattttata atgctgggtt gcatgataac gatctggccg tatcggttaa
     3601 tggttcagaa ttgcgtgata ccagacaggc gcagcagata atgaagcaat tgccagaact
     3661 taaagaaata aaaataaccg tcgagcgtga tggtcagtta tatgatgcat ttattgctgt
     3721 aggagaaaac tgattgttca ggaaatggtt gaatgggcgt ttgccggtac ttgtgttcac
     3781 tacagtaatt ttgggggcca ttccagggtg gggggctgaa ttttcggcca actttaaaga
     3841 tacggatatt caggagttca taaatactgt cagtaaaaat ttacacaaaa cggtaataat
     3901 taatcctgac gtgcagggaa ccatcactgt acgcagctac gatatgctga acgaggaaca
     3961 atattatcag ttctttctca gtgtgctgga cgtttatggt tttgctgtgg tcgatatgca
     4021 caacggtata ctgaaagtag tgcgctcaaa agatgccaaa acgtcggcgg tgccggtagc
     4081 tagtgatgtc agtcccggga ctggtgatga ggttgttacc cgggtggtcc ccgtaagtaa
     4141 cgtggcagcc agagatctgg cgcctttgct gcgtcagctc aatgataatg ctggcgcagg
     4201 aagcgtggtg cattatgaac cttctaatgt tttgttgatg accggacgtg ctgcagtgat
     4261 gaaacggttg atggagattg ttgaacgtgt ggataaggtg ggtaatcgca gcgttgccac
     4321 ggtcccgctc acctacgcgt ccgcaacaga cgtagccaga cttgttacgg aactgactaa
     4381 agaaacagat aagacagcta tacctgcttg gatgacggcg aaactggttg cagacgagag
     4441 gacaaactca gtgctcgtca gcggagagcc aatctcccaa cagcgtatca tctccataat
     4501 taagcaactg gatcgtcagg aggatgttca gggtaatact aaggtgattt acctgaaata
     4561 tgcgaaggcg aaggatttag tggaagtcct gacaggtatc agcagcagta ttgaaaacga
     4621 ctctaaaaag agtccgtcaa cggaagcctt gcgcaaagga gtgacgatta aatcccacga
     4681 acaaaccaat gccctgatcc tgacgggggc ccctgacgtc atccgcgacc ttgaaaatgt
     4741 gatttcgcag ttggatattc gtcgtcctca ggtcctggtg gaggccatca ttgctgaaat
     4801 acaggatgct gacgggctga accttgggat ccagtgggtg aataaacatg ccggtgtggc
     4861 gcagttcacc agtaccggtt tacctattac cacgatggtt cagactcgtc agaacgaaat
     4921 cttagacagc gatcagagca atgccctgag catgtttaac ggaattgcag cggggtttta
     4981 tcagggaaac tgggcgatgc tgttgacggc gctctccaca agtagcaaga atgatatctt
     5041 ggcgaccccc agtattgtca cgctggacaa tatggaggcc actttcaatg ttggtcagga
     5101 ggtcccggta ctttcgggct cacagacaac ctctggggac aatattttta acacggtcga
     5161 gcgcaaaacg gtggggatca aactcagggt aaaaccccag atcaacgagg gtgattccgt
     5221 gttactggag atagaacagg aggtgtccgg tgtggcggac actgcagtag ccaccactac
     5281 tgacttggga gcaaccttca acacccgaac agtgaccaat gccatgctgg tcgggaatgg
     5341 cgaaacggtg gtggtcggag gattactgga taagtcgatc agggggagtg agagtaaagt
     5401 gccactgctg ggggatatcc cggtactggg gcatcttttt cgcgcaaaaa gcgaacagac
     5461 agctaagcgt aatctgatgc tgttcattcg gccaactatt attcgtgagc gcgacggatt
     5521 tcgtcatgct tcggccgaaa aataccagtc gtttaatcag gaacaggtgc agtcgcgtgg
     5581 caaagaaaca acggcgctga cgctgaatga ggaacagctc aggctgtccc ccgatcaaga
     5641 cgatacggct ttccggaagg tgaaagcggc gattgctgcg ttttatgcgc aggagatgta
     5701 atgagcagag tagtacagaa tgtcagtgaa tcacgtcccc ttctgccttt ttccttttcc
     5761 cgtacgcaga gaattttgtt gctgcgtgaa caggagggga atcgagtgtt ctgcatggag
     5821 gacacacctg ccagtgccct gttggaggtg cgcagggtgg ctgaaggtcc tctgaatgtc
     5881 acaacagtgt cggcggaagc gtttgaaaaa cagctggtga gcagctatca acgtgattct
     5941 gatgaagcgc gacaaatgat ggctgagatt ggtaatgaaa tggattttta taccgtcgca
     6001 ggggagttgc ctgaccgtga ggacttactg gatgcgaatg acgatgcgcc aattatccgc
     6061 ctgattaacg caatgctgac agaggcaatt aaagagaaag cctcagatat tcatattgaa
     6121 acttacgaac gccatctgca ggttcgcttt cgcattgatg gcgttctgcg ggagatcctc
     6181 cgtttacata ggaatctggc ttcgttactg atttcacgca ttaaagtcat ggcccggttg
     6241 gatattgccg aaaaacgcgt tccccaagat ggccgcatgg tactgcgtat cggtggtcgg
     6301 gcggtggatg tgcgtgtttc aacgttgcct tcaaatcatg gtgaacgcat cgtgctgcgt
     6361 ttactagaca aaaatagcgt tagtctcgat cttgctgcac tcggcatgtc gcagcagaat
     6421 cagcgacaca ttgatgcact gatccgccgt cctcacggca ttattttggt gaccgggcct
     6481 acagggtcgg gtaaaagcac gacgctttat gccgcgttaa gcctgctgaa tccccgagac
     6541 cgcaacatta tgacggtcga ggatcctgtt gaatatgaac tggacggtat cagtcagacc
     6601 caggtcaacc cgaaggtgga catgaccttt gcccggagtc tgcgcgctat tttacgtcag
     6661 gatccggacg tggtgcttgt gggggagatc cgtgacggtg aaacagccca gattgctgtg
     6721 caggcctcgc tgacagggca tttggtgctg tcaacgttac atacgaatag tgcagcaggc
     6781 gcgctgtcgc gtctgcagga tatgggcatc cctccttttc tgctttccac ctcattactg
     6841 gctgttctgg cccagcgact ggttcgcacg ttgtgtccgc gctgtcgtca gccctgtcag
     6901 gtcagtactg agttagcgat ggacatggac attccccctg aaaccacgat ctggcagcct
     6961 gccgggtgtc agcactgcag tttcacgggc tatcacgggc gcaccgggat ccacgagctg
     7021 ttgctgattg acgatcgcat tcggacggca atctatcagg gggaggggga gctgggtatt
     7081 acccgcttgg cagggagtcg ctatctgacg ctgcgtggcg acgggcgaca aaaggtacta
     7141 gccggtgaga ccagttggga ggaggtggtt cgcgttactg aaagcagatt gcaggaagag
     7201 gaatgatatg gcgttgtttc attatcaggc atcagatatt cacggtagaa aacgcagtgg
     7261 cattctggag gcggattcgg cccgacatgc ccgtcagttg ctgcgtgagc aggcgcttat
     7321 tccagtcagg ttggatgaga aacaggtcca tcacaagcac tcactgcgga gtatcctgag
     7381 gtttcgtccg cgcgggggga gcagcgccga actcgcgtta ctgacacggc agttagccac
     7441 actagtggcc gcatctctgc cgcttgaaga ggcgctggat gcgttgttga ggcagagtga
     7501 aaaaccgcgt cagcggaact taatcgctgc cgtgcgcacc aaggttctcg agggacattc
     7561 cttggccgcg gcgatgggca tgtttccagg tacgtttgag cgtctttatt gtgcgatggt
     7621 ggcggcgggg gaaacatccg gtcgtttgga cgtggtgctc agtcgtctgg ctgactatac
     7681 cgagcagcgc cagatcatgc gaaaccgtct actccaggca ttgctctatc cttgtgttct
     7741 gacgttggtg gcggttgggg ttattgccat tctgcttact gcggttgtgc cgaaagtggt
     7801 tgagcagttt attcatatga aacagaccct tcccttatct acccgcgtac tgatgggggc
     7861 tgctgaggtg agccagactt ggggcccgtg gctgttactg gccgcagcgc tgggcgggat
     7921 agcggggcgc atgatattac atcagccctc ccagcgtctg gcttttcatc atctgctgtt
     7981 gcggctgccg gtcgtggggc gcatatcgcg cggtctgaat accgcacgtt atgcccgcac
     8041 gctgagcatt cttaatgcca gtgcggtacc gctgctccag gcgatgcaca tcagtggtga
     8101 cgtactgagt aatgactggg cccgtcatca gttagctacc gcggccgagt tggttaggga
     8161 aggggtcagc ctgcatcagg cactggagca gacttcgctg tttccgccta tgatgcggca
     8221 catgattgcc agcggtgaaa atagtggcga actcgacagc atgttggaac gggccgccga
     8281 caatcaggat cgtgagttca gcacacagat gcaactggcg ctgggattgt ttgaaccgct
     8341 gttggtggtg ggtatggccg gggtcgtttt gtttattgtt ctggcaattc tgcagccgct
     8401 cctgcagctc aacaacatga tgaatatgtg aatccttctt taacagtcaa cggagaaaaa
     8461 catgcgaaaa cagcatcagc gtggttttac cttgctggaa ataatggtgg taattgtgat
     8521 cttgggagtt ttggccagtc tggtggtccc gaatctgatg ggaaataagg ataaggccga
     8581 tcggcagaaa gtgatgagcg acttggttgc gctggaaagc acgctcgata tgtaccggtt
     8641 agataataac cggtatccga caacggagca gggactgcgg gcactggtca gtaaacccac
     8701 cgtgcaacct gaaccccgta actaccggca ggatggttat atccgccgtt tgccccagga
     8761 tccttggggg ggggattatc agctgttaaa tcctggtcag tacagcgaca ttgatatctt
     8821 ttctcctggc ccggatggtg tgccaaacac tgaagatgat attggtaact ggacgctcgg
     8881 taacgcgcag ccatgagtca gcgcggtttc acaatactgg agatgatgct gatccttttg
     8941 ttgatgggca tcactgcagg gctggtacta atgtcatttc ctgactctgc tcagaatcat
     9001 ctgcaacaac agcgggagcg tctgcaggcg cagctggatt atgccttgga tagaagccag
     9061 caggacggct tgctgatggg gattcaggtt cggtttgacg gatggaaatt taaggttctg
     9121 cagcgcggaa cagcggaaag ccccccgaca ttagctgagg gaggggatat ttggcagggg
     9181 tatgtctggc aaacttggca accgcgacgt gcagccatgg ggggcaaact tccggatgat
     9241 gtgcgtcttg agctgcagta tctcagaggt ttgcagtgga tgtctgagca tgatgacggg
     9301 gctgaacccg atattttgct gcttcccgga ggtgaggtaa cgccgttcag acttcttttc
     9361 aggcaggtcg gagaggaggc cgttgtcggg ctgcaggttg atgagaacgg tttgatgact
     9421 ctttttgaag gtgaggtctc attgtgaagg ggcaagaagg aatgacatta ctggaagtca
     9481 tagtagctct ggttgtgttt gcgctggcag gaatggcgct gatgcaggcc tcgacccagc
     9541 aggctgcggg gatcggacgg atggaagaga aggtgctggc aggctggttg gccgataacc
     9601 agatggtgca attgcaactc gagaaaactt ggccggaaaa tggctggggc gagaagacga
     9661 tcagcttcgc tggcaccgaa tggtatttac gctggggcgg gccggacagc gacgtgccgc
     9721 agccgcgctc cttagaggta gaggtacggc gcactaaaga ggagacgtcc gccttggttt
     9781 ccttgcgtag cagtgtggtt cgtgaatgag tcagcaacgg gtaaaaggtt ttacgctact
     9841 ggaaatgctg ttggcactgg ctgtttttgc agcgttgagc atcagcgcat ttcaggtgct
     9901 gcagggtgga atacgggcgc acgagctgtc ccgggataaa gtacagcgtc tggctgagct
     9961 gcagagaggg atcagccaga tggagcgaga tcttacgcag atgctcccaa ggcacagccg
    10021 ggggaatgaa ggcctgctgc tcgctgcacc tcatctgcta aaaagtgatg actgggggat
    10081 ttcttttacg cgaaacagct ggctgaatcc cgccgggatg ctgccgcgcc cggagttaca
    10141 gtgggtaggt tatcggttgc gacagcaaaa gttagaacgc ctgagctact ttcatgtcga
    10201 tcacccttcc ggtgtttctc cggatgtcag ggtgatgctg gacggcgttc acgcatttcg
    10261 tctgcgtttt tttgttaacg gtgattggca ggcgcgctgg gacagtaccg gcatcctgcc
    10321 gcaggctgtt gaagtgacgc tggttatgga tgattttgct gaactgcccc gtctgtttct
    10381 tgtgagcaag gagaccgctg aatgaaattg cgggaacagg gcgtggcgct tctggtggta
    10441 ttgctgatcc tgtcgttaat ggtaacgata gcggcggtga ttgcggagcg caacggtcgg
    10501 acatttttgc gtacagtagc gcagttggat cagctgcagg caaagtggga tggctatacg
    10561 gcagagacga tagcaaaaca aatcttacag aggagcaggc aggagtcgcc ccggaaaacg
    10621 catctggcac aaaattgggc gcagtcagaa cgacagtttg agaccagagg gggagatgtg
    10681 cgggggcaga tcgtggatgc gcaggcctgt tttaatctta atgctattaa ttatggagtg
    10741 gtggatctga ccagtatacc ttatgccgcc agaatttttc agcagctatt aatcaacttg
    10801 caggtagagc ttttacaggc ccggcaagtg acggcagccc tacgtgactg gatcgatcgt
    10861 gatgataagc cggtaagggg cggtgctgaa gacgaggtgt atatggggat ggaacctccc
    10921 tatctggcag ccaaccagcc gatgcaggac gtcagtgagt tacgccttat ccggggaata
    10981 gatgccaggc tttaccgcaa acttttgccg tatgtttgtg tactgcctac gtcagatctg
    11041 tctgtgaatg tgaatactct gctggattca caggcgccgc tgctggcggc gctctttctg
    11101 acgaaaccgg acagtcttcc ggtgacggaa ttactgcagc ggcgaccccg tactggttgg
    11161 gagagcgtgg cggcgtttct tgacgcgcca ccgcttaagg acattgacac cagtgcggcc
    11221 atgccggtat tggccgtcag cagcaactat tttctggttc ggttacatgt gcgctccggg
    11281 gaacacctgt tcagtcagca gactttaatg cagtggaggg aggagcgttt ccggatcatt
    11341 cagcgacagt atggattaac gatgagggag gttccctgaa aaaaaataac aaacatgtcg
    11401 aggctggtcg gctttttctg cgactgccgg aggatgactc ggagccttta ttgtggaggc
    11461 tgattgcgcc agaggagaca ggagccggca gtgtggcagt cggagaaacg gatccgcagt
    11521 tagtcggttt gctgtcccgt tatcccgcct gggtgctggt tccgtcaggc agaattgcct
    11581 tccatcaggt cacgctgcca caccgcacgt ggcgtcagcg tttgcaggtg ctgccctttc
    11641 ttctggaaga gcagttggcg acagacattg agcagctgca cttcgccatt cttcatcagt
    11701 cgggtgacag gtgtgatgtg gcggtggtcg aaaaagcggt tatgcatcgc tggttagcgt
    11761 actgtggaca acttggggtt cgggaggtga ggttgctgcc tgacgtactg atgctgccgt
    11821 tggcaactga aggatggagt gccgtaaagc ttgccgggca gtggctgttt cgccgggatc
    11881 gttacgccgg gatgacggta gagttatcgt ggctgacaca tttcttgtct cttactgcgc
    11941 cgccggtgat tgagtcctac tccgtgccgc cagtaccgtg cccgggtccg aagaccactg
    12001 agtggcgaaa tcagcccgga cgcgatctgc tgcagttggt ggccgaaggt gacgggtatg
    12061 atggcgcaga tctgcggcag ggagaatttg cccgccaggg aacatggtat gcacgatttc
    12121 gcccatggag gtatgtcgca ggggctctgc tggcctgtgt ttttctggcc gggagcaacg
    12181 cggggctggc acattatcgg ctctggcagc aggctcaatt ctggcggtat gagagcgtac
    12241 gtgtctatca gcagctgttt ccgagtgaaa aggcggtgaa ggatccccgc cggcagatgc
    12301 tgagacatct gcaacagtcg acgggtaaca atataccgga actcgggagc gtgatgcgcc
    12361 agcttcagca actgctgtca gaaacctcaa cgatacgctt acaggcactc gcctgggaca
    12421 gcagcagcca gacactgaaa attgatttgc aggcggcttc ttttcaggcg ctggaacact
    12481 ttcagcagat cgccggagaa aaatacctca tccagcctgg tgagatacga cagcagccgc
    12541 agggggtaga aagtcgtctg atcctgagag tgaacaatga acgagcttaa aaaacgtttg
    12601 cagtatgtta gcccccgtga gcgcaggctg cttatcggat gtgttgcctt actggttgtt
    12661 gtttttgtct attacgtcct ctggcagccc tggctgatac gagaggataa gtggcggact
    12721 gtagttaccc gtgaaaaaaa cacagtggag tggatgaaac aacaggtacc caacataaag
    12781 agacgggagg caagtatgcc tgaacagggg gagccgttag gtttatctgc ggccgtgaca
    12841 cgtacttcag ccgcatacgg tgtatcggtc acccggttgc agcctcaagg ggagcgtctg
    12901 gccgtcacct tagccccggt tgaatttaat gcgctgatgc agtggcttac gcaactggag
    12961 cgtcggcacc gtattcgtac catggtattt gatgtggccg cgcagggcaa cccacccgga
    13021 caggtaacgg ttaatcggct ggttttgtcc caagatgacg taaacaagga cgcttcgctt
    13081 agtcgctgat gtcgtaattt acagattaat gcttgatgta aaaattggac ttataaagga
    13141 ggatgtgtga gtagtttgcc ggaatttgcg gttgattatc ccttgttgtg gctcatctgc
    13201 accggagggc tggggggcgt taccggcagt tttcttaacg tcgtgattca tcgtctgccg
    13261 ataatgttgg agaggcggtg gcggcgtgaa gcgctgacgc tattggcaca ttctgtgcct
    13321 gagtccgggc cggcctttaa cctgatgata ccgcagtctc attgtccctg ctgttttcat
    13381 ccgctcggca tcagggataa cattcccctg ttaagctacc tatttctgaa ggggaaatca
    13441 cgctgttgtg gcgagcgtat tcccgtttac tacccgctag tcgaaataac gaactcagtg
    13501 ttattcattc tggccgcgag caggtttcca ccagggctga cattagcggc tgcctggctt
    13561 tttatttcca tgctgttggt tctggcggtt attgactgcc atacggcgct gctgccggat
    13621 gtgctgacgt tgccattgct ctggcttggc ctgttattca gtttacagag aggcgttgtc
    13681 accttagagg aggcggtagt gggggcggta tctgggtatc tctgtctgtg gggactgtac
    13741 tggctttttc gtttcgctac cgggagggaa gggttaggtt acggtgattt taagctagct
    13801 gcggcattgg gagcatggat gggttggcag gcattaccct caatactttt gtttgcctca
    13861 gtcagtgggt taatcgtaac agtactgttg cgtattgtaa cagctacgga ctttacccgc
    13921 ccgctgcctt ttgggccgtg gctcgctttg agcggagtgt gccatttttt gttgatgtag
    13981 cgttcggtta ccggggctag taataaaaac ggctcagcgg ataaaagaac tgttgtaaac
    14041 cggtatcagg ttaaacgata cctagtaaaa aatgatgggt aatattttga aaaaactgaa
    14101 ttgtatcgcg tcattactag tcttagtgac tatctctggt tgtcatcagt caccctccat
    14161 ccataagcag gccactgttc ctccgagcga acaactggaa caaatggcgt cgattgtctc
    14221 tgcgacacgg tatctgaaaa tgagatgtaa ccgtagtgat ttaccagatg aacagtcgat
    14281 cctaaatgtc gctaaccgga tcgctatcgg gaaagggtgg cagtcattaa ctcaggagga
    14341 tatacgtaaa cacagcgatg atatctatgt gcgtctgact agggacagta cgcctgagta
    14401 tataaaatgc agagaattca atcggcggct ggtcccattt atcggtgaat tattggcaag
    14461 gggtcgtgga taatatgaac cgccccggaa atcctggaga ctaaactccc tgagaaagag
    14521 gtaaacagca tgaataaaaa tgggtcttcc cttgtagtgg tggctgaagg catgataatt
    14581 gtagtggcac actgaatttg gccacctgaa cagaggtgat atgctcacct cagaacaaca
    14641 caggtgctcc aatgaaaaaa agaaatttca gcgcagagtt taaacgcgaa tccgctcaac
    14701 tggttgttga ccagaactac acggtggcag atgccgccaa agctatggat atcggccttt
    14761 ccacaatgac aagatgggtc aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag
    14821 cctctccgat aacaccagaa caaatcgaaa tacgtgagct gaggaaaaag ctacaacgca
    14881 ttgaaatgga gaatgaaata ttaaaaaagg ctacaaccgc gctcttgatg tcagactccc
    14941 tgaacagttc tcgataatcg ggaaactcag agcgcattat cctgtggtca cactctgcca
    15001 tgtgttcggg gttcatcgca gcagctacag atactggaaa aaccgtcctg aaaaaccaga
    15061 cggcagacgg gctgtattac gcagtcaggt acttgagttg cataacatca gccatggttc
    15121 tgccggggca agaagcatcg ccacaatggc aaccctgaga ggcttcagaa tggggcgctg
    15181 gcttgccggc aggctcatga aagaactggg actggtcagt tgccagcagc ctgcgcaccg
    15241 ttataaacga ggtggtcgtg aacatgtcac tatcccgaat caccttgggc ggcagttcgc
    15301 agtgacagag ccaaatcagg tatggtgcgg cgacgtgacg tacatctgga cggggaaacg
    15361 ttgggcatac cttgccgttg ttctcgacct gtttgcaagg aaaccggtag gttgggcaat
    15421 gtcgttctct ccggacagca gactgaccat caaagcgctg aaaatggcct gggaaatccg
    15481 cagtaaacca gccggggtaa tgttccacag cgatcagggc agccactata caagcaggca
    15541 gttccggcag ttactgtggc gttaccagat caaacagagt ctgagtcgac gaggaaattg
    15601 ctgggataac agcccgatgg agcgcttctt caggagtctg aaaaacgagt ggataccggt
    15661 gacgggttac atgaacttca gcgatgctgc ccatgaaata acggactata tcgttgggta
    15721 ttacaacgcg ctcaggccgc acgaatataa cggtgggttg ccaccaaatg aatcggaaaa
    15781 ccgatactgg aaaaactcta aagcggtggc cagtttttgt tgaccactac atcccgtcag
    15841 ccgatttacg ggccgtaagt aacgaagttg gatgcaaatg tcagatcgtg tgcgcctgtt
    15901 atggctcggc tggtaagaga gccttatagg cgcatttgaa aaaactccag ctataccgga
    15961 agagatttat tattaacagg agaggtttgc ttttctgtgt aggtttagat tttgtgtttg
    16021 cagggtaaac aaaactgatt aactcgtcat ttcggtaact gaaatagttt gctgtattct
    16081 gtttacaatc tctttaataa ggcatttgtc acgtggctat tcatatgaaa atcatacgtt
    16141 atcttacaaa tcaatcatct gagtgttata atataactta gctgtgatat gtgtaagaat
    16201 gtttaggcaa tatattatta tcgctaatgt tgttttggtt gggagagatt ctccttgcgg
    16261 tagctttctg tttttagatg cttcttgctt aaaaggatat aatttctgtt cttttatata
    16321 gggttcttta catttactga gttttcttat ttatttaaac attatgaaat ctaatgcttt
    16381 tgacgtcatg gggaaggttg catggctatg ggcctgttct cctctgcata aaaaatggcc
    16441 attatctgtt tttgctatta atgtcatacc tgctattcag acaaatcaat ttgctctctt
    16501 aataaaggat gagcttcctg tagctttttg tagctgggcc agtttagatc tggaatgtga
    16561 ggtaaaatat ataaatgatg taacatcttt gtatgcaaaa gactggatgt cgggagaaag
    16621 aaagtggttt attgactgga tagctccatt tggacacaac atggagttat ataaatacat
    16681 gaggaaaaag tatccttatg agttattcag agcaataagg ttagatgaga gcagtaaaac
    16741 aggaaaaata gctgaatttc atggaggggg gatagataaa aaactggcat caaaaatatt
    16801 caggcaatac catcatgaac ttatgtctga agta
//
LOCUS       DAZFDC010000103        16668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFDC010000103 DAZFDC010000000
VERSION     DAZFDC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.103"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(144..2093)
                     /locus_tag="ACHJ48_003487"
     CDS             complement(144..2093)
                     /locus_tag="ACHJ48_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HFO4708970.1"
                     /translation="MSQRYKLYRRTSGIYVVRISVPQRFRRYAGQCEIHTSTGTHDLH
                     EAKQKSALLLAVWYQTLQEYEQLDYRTLSDCAPLLAGEGMISLSNFAQSIELPISQLI
                     REVVNRNLPVFWLATGQFGFYVDEFNAVEREPGAKREKQSDDEKDQPKEVIILNSAFE
                     LGIESFANGYLRPFNPRHTLDCLLSAGVSEGEAAFRTSGDNQSGGWFFDLPGVDITAD
                     SLLISKVHAEGLRLTWLVKTTPPAVSIHPAVPLVAPVIANEYVHRKHYNENLSWLREE
                     YLKHRRKGKVSEAALRDIRYYFDLMIEVMGDIQLEDFDRDFLRAYESKLRTIPANRNL
                     MKGKHGVKTLDELIAKAAECGDKLMTEESVKKYINGLYGAMEWAVDDGKFLKSPCDNF
                     FPPDDKGEREQDHTDIFEPHEIKAIFSQPWFVAGTVERNAQGRFHQYCPFHYWAPLLG
                     LMTGARVNEIAQLMLDDVLADDGVYYLNLESDSENGKKLKNANSRRKIPVHSTLIELG
                     FIEYVDALKAAGYDRLFPELKPHKTKGYGRPVSAWFNESLLAGRLKLERDRSKSFHSF
                     RHSVSTLLKEKGVSSELRGQLLGHVRGKTETEVRYSKDLKPVHMVEVVEKIDFSLPEI
                     ARFNIPDGLDAVRDALRRKRGKQTG"
     gene            complement(2184..2268)
                     /locus_tag="ACHJ48_003488"
     tRNA            complement(2184..2268)
                     /locus_tag="ACHJ48_003488"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2232..2234),aa:Leu,seq:caa)
     gene            2464..3483
                     /gene="ahr"
                     /locus_tag="ACHJ48_003489"
     CDS             2464..3483
                     /gene="ahr"
                     /locus_tag="ACHJ48_003489"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO4708971.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3613..5115
                     /gene="yjgR"
                     /locus_tag="ACHJ48_003490"
     CDS             3613..5115
                     /gene="yjgR"
                     /locus_tag="ACHJ48_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO4708972.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIDVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5276..6358)
                     /gene="lptG"
                     /locus_tag="ACHJ48_003491"
     CDS             complement(5276..6358)
                     /gene="lptG"
                     /locus_tag="ACHJ48_003491"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO4708973.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6358..7458)
                     /gene="lptF"
                     /locus_tag="ACHJ48_003492"
     CDS             complement(6358..7458)
                     /gene="lptF"
                     /locus_tag="ACHJ48_003492"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO4708974.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7521..7571)
                     /gene="ytgA"
                     /locus_tag="ACHJ48_003493"
     CDS             complement(7521..7571)
                     /gene="ytgA"
                     /locus_tag="ACHJ48_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO4708975.1"
                     /translation="MSIIILATISTTQENK"
     gene            7725..9236
                     /gene="pepA"
                     /locus_tag="ACHJ48_003494"
     CDS             7725..9236
                     /gene="pepA"
                     /locus_tag="ACHJ48_003494"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO4708976.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9590..10033
                     /gene="holC"
                     /locus_tag="ACHJ48_003495"
     CDS             9590..10033
                     /gene="holC"
                     /locus_tag="ACHJ48_003495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO4708977.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10033..12888
                     /gene="valS"
                     /locus_tag="ACHJ48_003496"
     CDS             10033..12888
                     /gene="valS"
                     /locus_tag="ACHJ48_003496"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO4708978.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12943..14139)
                     /gene="yjgN"
                     /locus_tag="ACHJ48_003497"
     CDS             complement(12943..14139)
                     /gene="yjgN"
                     /locus_tag="ACHJ48_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO4708979.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGVWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIVSVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            14332..14835
                     /gene="yjgM"
                     /locus_tag="ACHJ48_003498"
     CDS             14332..14835
                     /gene="yjgM"
                     /locus_tag="ACHJ48_003498"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO4708980.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14881..15297)
                     /gene="rraB"
                     /locus_tag="ACHJ48_003499"
     CDS             complement(14881..15297)
                     /gene="rraB"
                     /locus_tag="ACHJ48_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO4708981.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            15459..16463
                     /gene="argF"
                     /locus_tag="ACHJ48_003500"
     CDS             15459..16463
                     /gene="argF"
                     /locus_tag="ACHJ48_003500"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO4708982.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKPFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(16520..>16668)
                     /locus_tag="ACHJ48_003501"
     CDS             complement(16520..>16668)
                     /locus_tag="ACHJ48_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708983.1"
                     /translation="FRMMQTILKSEVNVSPFIDQQRLNTLNPPENLRIAIEKFGWKKK
                     TITA"
BASE COUNT         4323 a         4206 c         4248 g         3891 t
ORIGIN      
        1 gagctaatgc aaatcaagtc tcaccagacg cattttaagc gtctccaaat ttggcactat
       61 taaatccacg acctttaata aaaaatgcgc caggaagcgt cccagcgcat tcaagagcat
      121 aactcaactg aatagtaact gactcaacct gtttgtttgc cacgctttct tcgcagcgca
      181 tctcttacag catccagccc atcaggaatg ttgaatctcg ctatctcggg caaagaaaaa
      241 tcaatctttt cgacaacctc aaccatgtga accggtttta aatctttgct gtatcgcact
      301 tcagtttctg ttttgcctcg cacatgccca agtagctgcc cacgcagttc cgaactaaca
      361 cccttctctt taagcaaagt tgaaacagaa tgccggaaag agtggaaaga tttgcttctg
      421 tctctttcaa gttttaatcg acccgcaagc aatgattcat tgaaccatgc ggaaaccggc
      481 ctaccatagc ctttggtttt atgtggttta agctcgggaa aaagacggtc atacccggca
      541 gctttcaacg catccacata ctcgataaaa ccgagttcaa tcagcgtaga atgaaccgga
      601 atcttgcggc gggaattggc gttttttagt ttctttccgt tttcgctatc gctttcaagg
      661 ttcaggtaat aaacgccgtc atctgccaga acatcgtcca gcattaactg tgcaatctcg
      721 ttaacccttg cccccgtcat caagcccaac aacggcgccc aatagtgaaa cgggcaatat
      781 tgatggaatc gcccttgcgc attacgttca acagttccag cgacaaacca cggttgcgaa
      841 aaaattgcct taatttcatg cggttcaaat atgtcagtgt gatcctgctc tcgctcacct
      901 ttgtcatcgg gagggaaaaa gttgtcgcat ggcgatttca gaaacttacc atcatcaaca
      961 gcccactcca ttgcaccata aaggccgttg atatactttt tgacagactc ttctgtcatc
     1021 agtttatcgc cacattctgc cgctttggcg attaactcat ccagcgtctt aaccccgtgc
     1081 ttacctttca tcaaattacg gttagcagga attgtgcgca acttgctctc ataagcccgg
     1141 aggaaatcac ggtcgaaatc ttccaactga atatccccca tcacttcaat catcaaatcg
     1201 aagtaatagc gaatatcgcg gagcgccgct tctgatacct tgcccttacg tcgatgtttc
     1261 aaatactctt cacgaagcca tgacaagttt tcattgtaat gtttgcggtg aacatattca
     1321 ttagcgataa caggagcgac aagaggcacg gcagggtgaa tgctaactgc tggtggcgtg
     1381 gtcttaacca gccatgtaag tcgaaggcct tcagcatgaa ctttgctaat caacaggcta
     1441 tcagcagtta tatctacgcc gggtaaatcg aagaaccaac ctccactttg gttatcacca
     1501 ctagttcgaa atgcagcctc tccttcggat actccagcgc tcaacagaca atctaaagta
     1561 tgccggggat tgaaggggcg gagataacca tttgcgaagc tctcgatacc cagctcaaac
     1621 gcgctattga gaatgatgac ttctttaggt tgatcctttt catcatcaga ctgtttttct
     1681 cgttttgcac ccggttcccg ctctactgca ttaaattcat caacatagaa accgaactga
     1741 ccagtcgcca gccagaatac cgggaggtta cgattaacca cctctcgaat caattgcgat
     1801 ataggcaact cgattgattg agcaaagtta gaaagcgaga tcataccctc accagcaagc
     1861 agtggggcgc agtcacttaa agttcgataa tccaattgtt catactcttg taaggtctga
     1921 taccagaccg ccaacaggag cgcggacttc tgctttgctt catgaagatc gtgagtgcct
     1981 gttgaagtgt gaatctcaca ctgtcctgcg tatcgacgaa aacgttgggg aacgctaatg
     2041 cggacaacat aaatgccgct ggttcgacga tagagtttat atcgctgaga catcgcctcg
     2101 tatacccttt tgtaagaggg gctacgcgat ttcaagcaag attatgaatt aaattgtttt
     2161 aaaacaatag tttagatgag tcttggtgcc gaaggccgga ctcgaaccgg cacgtatttc
     2221 tacggttgat tttgaatcaa ctgcgtctac cgatttcgcc acttcggcac tgaagaggta
     2281 tgcggaaaac gttgtggatt atacctgtcg cacgccacca tgcaagcgcc agcttgctcg
     2341 cacctcgcta agtgctgaaa ttttcagcat tgcatacagc gatgtgtaac ctttgtcaca
     2401 ctccaggcac cccgccctgc catgctctac acttcccaaa caacaccaga gaaggaccaa
     2461 aaaatgtcga tgataaaaag ctatgccgca aaagaagcgg gcggcgaact ggaagtttat
     2521 gagtacgatc ccggtgagct gaggccacaa gatgttgaag tgcaggtgga ttattgcggg
     2581 atctgccatt ccgatctatc gatgatcgac aatgaatggg gattttcaca atatccgctg
     2641 gttgccgggc atgaggtgat tgggcgcgtg gtggcgctcg ggagcgccgc gcaggataaa
     2701 ggtttgcagg tcggccagcg tgtcgggatt ggctggacgg cgcgtagctg tggtcactgc
     2761 gacgcctgta ttagcggtaa tcagatcaac tgcgagcaag gtgctgtgcc gacgattatg
     2821 aatcgcggtg gctttgccga gaagttgcgt gcggactggc aatgggtgat tccactgcca
     2881 gaaaatattg atatcgagtc cgccgggccg ctgttgtgcg gcggtatcac ggtctttaaa
     2941 ccactgttga tgcaccatat cactgctacc agccgcgttg gggtaattgg tattggcggg
     3001 ctggggcata tcgctataaa acttctgcac gcaatgggat gcgaggtgac ggcctttagt
     3061 tctaatccgg cgaaagagca ggaagtactg gcgatgggtg ccgataaagt ggtgaatagc
     3121 cgcgatccgc aggcactgaa agccctgtcg gggcagtttg atctcattat caatactgtg
     3181 aacgtcagcc tcgactggca gccttatttt gaggcgctga cctatggcgg taatttccat
     3241 acggtcggtg cggttctcac gccgttgccg gttccggcct ttacgctgat tgcgggcgat
     3301 cgcagtgtct ctggctctgc taccggtaca ccttatgagt tgcgtaagct gatgcgcttt
     3361 gccgcccgca gcaaggttgc gccgactacc gaattgttcc cgatgtcgaa aattaacgat
     3421 gccatcaagc atgtgcgcga cggtaaggcg cgttaccgcg tggttctgaa agctgacttc
     3481 tgatctcttt gccgggtgcc tgtcgcccgg cacttaaatc tctttggaag gcgtctcgca
     3541 atctgctgaa tttagcggtg aagagtgcgc agccgttgcc tatactccag gcaggaaact
     3601 ggaggatatc tcatgagcga acccctgtta attgcccgca cgccggacac agaactattt
     3661 ttactgccgg gaatggctaa ccgtcacggg ctgattactg gcgcaacggg gacgggtaaa
     3721 actgtcacgc tgcaaaaact ggcggagtca ttgtcggaaa tcggcgtgcc ggtgtttatg
     3781 gctgatgtga aaggcgatct gaccggtatc gcgcaggcag gaacggcgtc ggaaaaactg
     3841 ctcgcaaggc ttaaaaatat cgacgtcaat gactggcaac cgcatgctaa tccggtggtg
     3901 gtgtgggata tctttggcga gaaaggccat tcggtacggg cgacggtttc agacctgggg
     3961 ccactgttgc tggcgcggct gttgaatctc aacgatgtgc aatctggcgt gctgaatatc
     4021 atcttccgca ttgctgacga tcaggggctg ttactgctcg actttaaaga tctgcgggcg
     4081 attacccagt acatcggcga taacgccaaa tctttccaga atcagtacgg aaatatcagt
     4141 agcgcatcgg ttggtgccat ccagcgcggg ctgttgtcgc tggaacagca aggcgcagca
     4201 cacttctttg gtgagccgat gctggatatc aaagactgga tgcgcaccga tgccaacggt
     4261 aaaggcgtta tcaatatcct cagcgccgag aaactttatc agatgccgaa actgtacgcc
     4321 gccagcctgc tgtggatgct ctcagagttg tatgaacaat tgccggaagc gggcgatctg
     4381 gagaaaccaa aactggtgtt tttcttcgac gaagcacatc tgctgtttaa cgatgcaccg
     4441 caggtactgc tggataagat tgagcaggtg ataaggctta ttcgctcaaa aggcgtgggc
     4501 gtctggttcg tttcgcaaaa cccgtctgat attccggata atgtgctcgg ccagctcggt
     4561 aatcgcgttc agcacgcttt gcgggctttt acgcccaaag atcagaaagc agtaaaagct
     4621 gcggcgcaaa ccatgcgggc caatccggcg tttgataccg aaaaggcgat tcaggaactg
     4681 ggcaccggcg aggcgttgat ctcttttctg gatgcgaaag gaagcccttc tgtggtggag
     4741 cgtgcgatgg tgatcgcgcc ttgttcgcgg atggggccgg tgacggaaga tgagcgtaat
     4801 ggcttgatta atcactctcc ggtgtatggc aaatatgagg atgaggtgga ccgggaatcc
     4861 gcctatgaga tgttgcaaaa aggctttcag gccagtaccg agcagcaaaa taatcccccc
     4921 gcgaaaggga aagaggtagc ggtggatgac ggcattcttg gtggattgaa ggatattttg
     4981 ttcggcacta ccggaccacg cggcgggaag aaagatggtg tggtgcaaac aatggccaaa
     5041 agcgccgctc gccaagtgac gaatcagatt gtacgcggga tgttggggag tttgctgggg
     5101 gggagaagaa ggtaagtcag gcgagatttg tctttgcctg atgcgacgct ggcgcgtctt
     5161 atcatgccca ccccactgca atatattgaa ttttaattat ttttcaggaa tgaacgaagc
     5221 acactttgct aacaataata aagggagctt tcgctccctt tattcgttca ttcggttacg
     5281 attttctcat taacagccac aggctgatta agaagaagct ggcgcttggc aacagtgcgc
     5341 cgatgatcgg cgggatgcca taaaccaacg tcagcgggcc gaagatctgg tccagtacgt
     5401 agaagacaaa accgaaactg ataccggtga ccacacgcac gcccatcggt acgctacgca
     5461 gtgggccaaa gatgaacgac agcgccatca gcatcatcac cgccacggat agcggctgga
     5521 agattttgct ccacatgttg agctgataac gtccggcatc ctgaccgctc gacttcagat
     5581 acttcacata gttgtgcaaa ccgctgatag agagtgcatc cgggtccagc gccaccacgc
     5641 ccagtttgtc cggcgtgagg ttggttttcc aggtgccgct caccgtctgc gaaccggtaa
     5701 tctgtttcgg attggtcaga tcagattcat caacctgcga caaacgccag actttatgtt
     5761 ccgggtcaaa cttcgcagta gcggcatagc gtacggattg cagacgacga ttctcgttaa
     5821 aggcataaat gctgatgcca cctaactctt cgtcaccttt aacccgctca atgtagacga
     5881 agttgttgcc atctttcgcc cataagcctt gctgggtaga gagcaacgag ccgccgtaca
     5941 tcgcctgcgc acggtagtta cgcgccatct gctcgccctg cggcgcgacc cattcgccaa
     6001 tcgccatcgt cagcaagacc agcggaatgg cggttttcat caccgacagc gccacctgca
     6061 tacgggtaaa accagaagcc tgcatcacca ccagttcgct gcgctgcgcc agcatcccaa
     6121 gaccaagcaa cgccccaagc agagccgcca tcgggaagaa tatttgcaca tctttcggca
     6181 cgctcagcaa ggtatacatt cctgcgccta acgcgtcgta actcccctgc ccggcttttt
     6241 tcaactgatc gacaaacttg ataatgcccg acagcgacac cagcatgaac agcgtcatca
     6301 tgatggtggt gaaaatagtt ttaccgatat agcggtcaag tacgccaaaa ggttgcatca
     6361 caccgctcct ttacgcgaaa aactggcgcg caggcggcgg accggcacgg tgtcccaaag
     6421 gttgagaaca atcgccaaag ccagataaat caggttaacg gtccacatcc acagcgtcgg
     6481 gtccagctta cctttaccgc cgttcgattt cagggaggtc tggatcagga agaaaagtag
     6541 atacagcagc atggctggca gcatcgacag tacgcgtccc tgacgtgggt taaccacgct
     6601 cagcggtacg accataagtg ccatcataaa cacggtgaat accaacgtga tacgccagtt
     6661 cagttctgcg cgagcacgat cggtgtcagt gttccacaat gtgcgcatgt ccatctggtc
     6721 ggtatcgttc gggtcgagcg ccaccgcctg gtgaccaatg atcgcctgat aatcctggaa
     6781 atccgtaatg cggaaatcac gtaacagtgc agtgccttcg aagcgcgttc cctggttgag
     6841 agtgacgacc tgggagccgt cgcgcagctg ggttaaatgt ccggaatcgg ccaccaccac
     6901 agaaggacgt gcattacctt ttggtcgaat ttgcgcgagg aacacatctt tgaaatcgct
     6961 gccgtcaacg ctttcgatga acagcaccga actgccatta gtcgcttgct ggaattgccc
     7021 ttgcgccagc gccgccatgc cagggttcgc tttcgcttct gctaacactt catcctgatg
     7081 acgcgatgac cacggtcccg cccacatcac gttaaccgcc gcgacgattg ccgtgaatac
     7141 cgcaaggatc attgccgctt tcaccagaac cgctttgctc aggccgcagg catgcattac
     7201 cgtaatttca ctttcggtat acagtttgcc cagcgtcatc agcagcccga ggaacaggct
     7261 taatggcagg ataagctgcg ccatttccgg cacgcccaac ccgagaaggg agagcaccag
     7321 attcgccgga atatcgccgt caaccgctgc gccgaggatc ctcactaact tttgacagaa
     7381 gaagatcaaa agcaagatga agagtatcgc cagctggctt ttgagcgtct cccgcaccag
     7441 atatcttatg attatcactt taaatacgcc cgtaaaaact cgtcttttgc aggattttag
     7501 cttgtttcat ggcttaaacg tcatttattc tcttgagtcg tcgaaatcgt cgctaagata
     7561 attatactca acggattcac ctctcagatt ttgttctgac gtgccaatgc cgtaataacg
     7621 ttaagattaa cacgaagtca tcgcaacagc ggacatgagt tacgaaagct tgcaattcta
     7681 tctgtagcca ccgccgttgt ctttaagatt caggagcgta gtgcatggag tttagtgtaa
     7741 aaagcggtag cccggagaaa cagcggagtg cctgcatcgt cgtgggcgtc ttcgaaccac
     7801 gtcgcctttc tccgattgca gaacagctcg ataaaatcag cgatgggtac atcagcgccc
     7861 tgctacgtcg gggcgaactg gaaggaaaac cggggcagac attgttgctg caccatgttc
     7921 cgaatgtact ttccgagcga attctcctta ttggttgcgg caaagaacgt gagctggatg
     7981 agcgtcagta caagcaggtt attcagaaaa ccattaatac gctgaatgat actggctcaa
     8041 tggaagcggt ctgctttctg actgaactgc acgttaaagg ccgtaacaac tactggaaag
     8101 tgcgtcaggc tgtcgagacg gcaaaagaga cgctctacag tttcgatcag ctgaaaacga
     8161 acaagagcga accgcgtcgt ccgctgcgta agatggtgtt caacgtgccg acccgccgtg
     8221 aactgaccag cggtgagcgc gcgatccagc acggtctggc gattgccgcc gggattaaag
     8281 ccgcgaaaga tctcggtaat atgccgccga atatctgtaa cgccgcttac ctcgcttcac
     8341 aagcgcgcca gctggctgac agctacagca agaatgttat cacccgcgtt atcggcgaac
     8401 agcagatgaa agagctgggg atgcattcct atctggcggt cggtcagggt tcgcaaaacg
     8461 aatcgctgat gtcggtgatt gagtacaaag gcaacgcgtc ggaagatgca cgcccaatcg
     8521 tgctggtggg taaaggttta accttcgact ccggcggtat ctcgatcaag ccttcagaag
     8581 gcatggatga gatgaagtac gatatgtgcg gtgcggcagc ggtttacggc gtgatgcgga
     8641 tggtcgcgga gctacaactg ccgattaacg ttatcggcgt gttggcaggc tgcgaaaaca
     8701 tgcctggcgg acgagcctat cgtccgggcg atgtgttaac caccatgtcc ggtcaaaccg
     8761 ttgaagtgct gaataccgac gctgaaggcc gcctggtact gtgcgacgtg ttaacttacg
     8821 ttgagcgttt tgagccggaa gcggtgattg acgtggcgac gctgaccggt gcctgcgtga
     8881 tcgcgctggg tcatcacatt accggtctga tggcgaacca taatccgctg gcccatgaac
     8941 tgattgccgc gtctgaacaa tccggtgacc gcgcatggcg cttaccgctg ggtgacgagt
     9001 atcaggaaca actggagtcc aattttgccg atatggcgaa cattggcggt cgtcctggtg
     9061 gggcgattac cgcaggttgc ttcctgtcac gctttacccg taagtacaac tgggcgcacc
     9121 tggatattgc aggaaccgcc tggcgttctg gtaaagcaaa aggcgcaacc ggtcgtccgg
     9181 tagcgttgct ggcacagttc ctgttgaacc gcgctgggtt taacggcgaa gagtaattgc
     9241 gtcaggcaag gctgttattg ccggatgcgg cgtgaacgcc ttatccgacc tacacagcac
     9301 tgaactcgta ggcctgataa gacgcgcaag cgtcgcatca ggcaatgctg ttattgtcgg
     9361 atgcggcatg aacgccttat ccgacctaca cagcaccgaa ctcgtaggcc tgataagacg
     9421 cggcagcgtc gcatcaggca atgctgttat tgtcggatgc ggcgtgagcg ccttatccga
     9481 cctacacaca gcactgaact cgtaggcctg ataagacaca acagcgtcgc atcaggcgct
     9541 gcggtgtata cctgatgcgt atttaaatcc accacaagaa gccccatata tgaaaaacgc
     9601 gacgttctac cttctggaca atgacaccac cgtcgatggc ttaagcgccg ttgagcaact
     9661 ggtgtgtgaa attgccgcag aacgttggcg cagcggtaag cgcgtgctca tcgcctgtga
     9721 agatgaaaag caggcttacc ggctggatga agccctgtgg gcgcgtccgg cagaaagctt
     9781 tgttccgcat aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg agatcgcctg
     9841 gccgcaaaaa cgcagcagca gcccgcgcga tatattgatt agtctgcgaa caagctttgc
     9901 agattttgcc accgctttca cagaagtggt agacttcgtt ccttatgaag attctctgaa
     9961 acaactggcg cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc tgaatacggc
    10021 aacctggaaa taatggaaaa gacatataac ccacaagata tcgaacagcc gctttacgag
    10081 cactgggaaa agcagggcta ctttaagcct aatggcgatg aaagccagga aagtttctgc
    10141 atcatgatcc cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc cttccagcaa
    10201 accatcatgg ataccatgat ccgctatcag cgcatgcagg gcaaaaacac cctgtggcag
    10261 gtcggtactg accacgccgg gatcgctacc cagatggtcg ttgagcgcaa gattgccgca
    10321 gaagaaggta aaacccgtca cgactacggc cgcgaagctt tcatcgacaa aatctgggaa
    10381 tggaaagcgg aatccggcgg cacgattacc cgtcagatgc gccgtctcgg caactccgtc
    10441 gactgggaac gtgaacgctt cacgatggac gaaggcctgt ccaatgcggt gaaagaagtt
    10501 ttcgttcgtc tatataaaga agacctgatt taccgtggca aacgcctggt aaactgggac
    10561 ccgaaactgc gcaccgctat ctctgacctg gaagtggaaa accgcgaatc gaaaggttcg
    10621 atgtggcaca tccgctatcc gctggctgac ggtgcgaaaa ccgcagacgg taaagattat
    10681 ctggtggtcg cgactacccg tccggaaacc ctgctgggcg atactggcgt agccgttaac
    10741 ccggaagatc cgcgttacaa agatctgatt ggcaaatatg tcattctgcc gctggttaac
    10801 cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac cggctgcgtg
    10861 aaaatcactc cggcgcacga ctttaacgac tatgaagtgg gtaaacgtca cgcgctgccg
    10921 atgatcaaca tcctgacctt tgacggcgat atccgtgaaa gcgcccaggt gttcgatacc
    10981 aaaggtaacg aatctgacgt ttattccagc gaaatcccgg cagagttcca gaaactcgag
    11041 cgttttgctg cacgtaaagc ggtcgttgct gcggttgacg cactcggcct gctggaagaa
    11101 attaaaccgc acgacctgac agttccttac ggcgaccgtg gcggcgtagt tatcgaacca
    11161 atgctgaccg accagtggta cgtacgtgcc gatgtactgg cgaagccggc agttgaagcg
    11221 gttgagaacg gcgacattca gttcgtaccg aagcagtacg aaaacatgta cttctcctgg
    11281 atgcgcgata ttcaggactg gtgtatctct cgtcagctgt ggtggggtca ccgtatcccg
    11341 gcatggtacg acgaagcggg taacgtttat gttggccgca acgaagaaga agtgcgcaag
    11401 gaaaataacc tcggtgccga cgttgccctg cgtcaggacg aagacgttct cgacacctgg
    11461 ttctcttctg cgctgtggac cttctctacc ctaggctggc cggaaaacac cgacgcactg
    11521 cgccagttcc acccaaccag cgtgatggta tctggtttcg acatcatctt cttctggatt
    11581 gcccgcatga tcatgatgac catgcacttc atcaaagatg aaaatggcaa accgcaggtg
    11641 ccgttccaca ccgtttacat gaccggtctg attcgtgacg acgaaggcca gaagatgtcc
    11701 aaatccaagg gtaacgttat cgacccgctg gatatggttg acggtatttc gctgccagaa
    11761 ctgctggaaa aacgtaccgg caatatgatg cagccgcagc tggcggacaa aatccgtaag
    11821 cgcaccgaga agcagttccc gaacggtatt gagccgcacg gcaccgatgc cctgcgcttc
    11881 accctggcgg cactggcgtc taccggtcgt gacatcaact gggatatgaa gcgtctggaa
    11941 ggctaccgta acttctgtaa caagctgtgg aacgccagcc gctttgtgct gatgaacacg
    12001 gaaggtcagg attgcggctt caacggcggc gaaatgacgc tgtcgctggc ggaccgctgg
    12061 attctggcgg agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga cagcttccgc
    12121 ttcgatatcg ccgcaggcat tctgtatgag tttacctgga accagttctg tgactggtat
    12181 ctcgagctga ccaagccggt aatgaacggt ggcaccgaag cagaactgcg cggtactcgc
    12241 catacgctgg tgactgtact ggaaggtctg ctgcgcctcg cgcatccgat cattccgttc
    12301 atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc cgacaccatc
    12361 atgctgcagc cgttcccgca gtacgatgca tctcaggttg atgaagccgc actggccgac
    12421 accgaatggc tgaaacaggc gatcgttgcg gtacgtaaca tccgtgcaga aatgaacatc
    12481 gcgccgggca aaccgctgga gctgctgctg cgtggttgca gcgcggatgc agaacgtcgc
    12541 gtaaatgaaa accgtggctt cctgcaaacc ctggcgcgtc tggaaagcat caccgtgctg
    12601 cctgccgatg acaaaggtcc ggtttccgtt acgaagatca tcgacggcgc agagctgctg
    12661 atcccgatgg ctggcctcat caacaaagaa gatgagctgg cgcgtctggc gaaagaagtg
    12721 gcgaagatcg aaggcgaaat cagccgtatc gagaacaagc tggcgaatga aggctttgtc
    12781 gcccgcgcac cggaagcggt catcgcgaaa gagcgtgaga agctggaagg ctatgcggaa
    12841 gcgaaagcga agctgattga acagcaggct gttatcgccg cgctgtaatc aaaacacagt
    12901 gatgaaaacg aaggccggag catgctccgg cctttttatc tcttacagaa atggtaaaga
    12961 aggcataatt ccccgtgaaa tactggctaa gaagccttta tctggttgtt cttctttatt
    13021 gattaaagga aggtcatcca aatcgcccag caaatacgta ttttttgcct gccagtcaat
    13081 catccatatt ttcagcagtg gataagccag accgcccgta atcccggata tcaccaccaa
    13141 cgcacacatg cgataaagca taccgtggta tgttaaagtt gagcgaaaac gaatacgccc
    13201 atcattcagt gacaggttgc tcataaaatg atttcgcaac gacaccgtta aataactggt
    13261 gctaacagca atcccaaaat aatagattaa ctgcgcgatt atcattttac gttgcatttc
    13321 cataaattgc tgtaaattct caatatcatc atttgcatac acagaactat catacgcatt
    13381 taatatttga tcgaagataa tataaccagc aacagcgaga aaaggtaata aagctaaaaa
    13441 tgcgaggatg gcatatttta tacaatacgc agtatctaat ttcaccttga aacgatgtat
    13501 accgaaactg gtattgctcc agagaaaact cattaccagg ctatataaag taccattaaa
    13561 aataccaatg gaaacaatac caactattgc catcagaacc acagatacaa taacactact
    13621 tgaactattg gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc
    13681 cattaaaatc ggcaagaaaa aggttaccca ccagacccct ttcatagagc aattaaaact
    13741 aaatcttacg ccgttgagac tggtcatcaa ggcctgataa cgcagtcctt ttgctgccat
    13801 aaatataagc aaaaccaaca gtgacaaagt caaaacacag ccaataagcg gcatatctgc
    13861 tgacactgtc attaagattg cgaaatagaa aaaaacaaaa acaagacaac taaaaaaaac
    13921 attcccacca gtaattccat aagaaaatcg ttgtccgtta acttccatat tagcatagag
    13981 ataacgctta catttcatta atgcccatgg taaatagata cctagcgtta taatcgttaa
    14041 caacacattc accacacaaa taagaaaata tctctcacct gtaccatgaa aaacaaacga
    14101 atgggacgga acatccattt cattaataac ttgagccata aaaattccct gaattaaaaa
    14161 atccattgat ataatttata gatacagaca gtaaaatgtt ctgccacgtt acaatactct
    14221 tatcagtaat aggctggcaa aaactttatt gctttataag acgcttgcgc acgtaagaag
    14281 agagtggtat taaacaaatg tataattact ttatttttat tgggttaaac tatgaataac
    14341 attgcgccgc aatcacctgt aatgcgtcgc ctgacgctgc aggataatcc tgctatcgcc
    14401 cgcgtcattc gtcaggtatc cgccgaatac ggtcttaccg ctgataaagg ctacaccgtc
    14461 gccgatccga atcttgacga gctgtatcaa gtgtatagtc agccgggtca tgcatattgg
    14521 gtggttgagt acgagggtga agtggtcggc ggcggtggga ttgcgccatt agcaggtagt
    14581 gaatcggaca tttgcgaact gcaaaagatg tattttcttc cggctattcg cggcaaaggg
    14641 ctggcaaaaa aactggcctt aaaggcgatg gaggaggcaa gagaaatggg cttcaaacgc
    14701 tgctatctgg aaacgaccgc ttttttgaaa gaagcgattg gtttgtatga acatttaggt
    14761 tttcaacata tcgactatgc acttggctgc acgggccatg ttgattgtga agtgcggatg
    14821 ctacgcgaac tctaaaaaaa tgcccgctag tgtgtgctat cgggcatttt aatcaattaa
    14881 ttagtggcga accccgtcat cgtcttcatc gacaaaatct tcatcgtcgc cgtcttcgcc
    14941 gttgggatct tcaaagtaag tgccccaacc gtcgtactca acgtcaaatt tctccgccag
    15001 cgtcatcagt tgttcaacct gggcatcgat cagatcggca ttcaacgcgc actcgctgag
    15061 gatgtcgcag caaatcacga tatcaccatc ttcaacttcc agctcttctg gatcggtcac
    15121 ttcgtaaccg agtttaaacg cttcaactgc tgctttttcc agggtttcca gatcgtctgc
    15181 ggaaagatgg tgttcgatgg tgtacagcgc gtccggatcg ctgccatctt ccagtaattc
    15241 ttcaataatc aaacgtgttt cttcacgctg ttcttccagt tgttccgggt ttgccatggc
    15301 tcattcctct taatgtcctg ccgatactca tattgtcaca caccactggc attgcctcca
    15361 cctttgtgac aaagatttat gctttagact tgcaaatgaa taatcatcca tataaattga
    15421 attttaattc attgagacgt tagccacagg agggatctat gtccgggttt tatcataagc
    15481 atttcctgaa attactcgat ttcacgccag ctgaactcaa cagcctgctg cagttagccg
    15541 cgaagctgaa agccgataag aaaagcggca aagaagaagc caaactcact ggtaaaaaca
    15601 tcgcgctcat cttcgaaaaa gactcgaccc gtacccgatg ctctttcgaa gttgccgcat
    15661 atgaccaggg tgctcgcgtt acttatctcg gcccaagcgg cagtcagatt ggtcataaag
    15721 agtcgattaa agacactgcc cgcgtgctgg gtcgcatgta tgacggtatt cagtatcgcg
    15781 gctatggtca ggagattgtc gaaacactgg cggaatacgc tggcgtgccg gtatggaacg
    15841 gcctgaccaa tgagtttcac cccacgcagt tgctggcgga tctcctcacc atgcaggagc
    15901 atttacccgg taaaccattc aacgaaatga cgctggtcta tgcaggtgac gcacgtaaca
    15961 acatgggcaa ttcgatgctc gaagctgcgg cgcttaccgg tctggatttg cgtctggtcg
    16021 cgccacaggc atgctggccg gaagctgcgc tggttacgga atgccgcgcc ctggcacaac
    16081 aaaatggcgg gaatattacg ctgacggaag atgtagctaa aggcgttgaa ggtgctgact
    16141 ttatctatac cgatgtatgg gtgtcgatgg gtgaagcaaa agaaaaatgg gctgaacgca
    16201 ttgcattact gcgtgattat caggtgaaca gcaagatgat gcagttgacc ggtaacccgg
    16261 aggtcaaatt cctccactgc ctgcccgcgt tccatgatga ccaaacgacg cttggcaaga
    16321 aaatggcgga agagtttggc ctacatggcg gaatggaagt gactgatgag gtcttcgaat
    16381 ctgccgccag cattgttttt gatcaggcgg aaaaccgcat gcacaccatc aaagcggtga
    16441 tggtcgcgac actcagtaaa taattgatat cgctataaag atgccgggaa atacagaaac
    16501 atcccggcgt caaattattt tatgcagtga tagttttttt cttccagcca aatttttcta
    16561 ttgctatacg taaattttcc ggaggattta gtgtatttaa acgctgttgg tcaataaatg
    16621 gcgatacatt aacctccgat ttcaagatag tttgcatcat tctaaacc
//
LOCUS       DAZFDC010000104        16247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFDC010000104 DAZFDC010000000
VERSION     DAZFDC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.104"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..368)
                     /locus_tag="ACHJ48_003502"
     CDS             complement(<1..368)
                     /locus_tag="ACHJ48_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4708984.1"
                     /translation="MQDIPQETHHETTRLTQSAQAVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSSARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
     gene            complement(378..707)
                     /locus_tag="ACHJ48_003503"
     CDS             complement(378..707)
                     /locus_tag="ACHJ48_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4708985.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            complement(707..3772)
                     /locus_tag="ACHJ48_003504"
     CDS             complement(707..3772)
                     /locus_tag="ACHJ48_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4708986.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMPGAVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEARKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            complement(3744..4073)
                     /locus_tag="ACHJ48_003505"
     CDS             complement(3744..4073)
                     /locus_tag="ACHJ48_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4708987.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            complement(4082..4468)
                     /locus_tag="ACHJ48_003506"
     CDS             complement(4082..4468)
                     /locus_tag="ACHJ48_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4708988.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            complement(4529..5272)
                     /locus_tag="ACHJ48_003507"
     CDS             complement(4529..5272)
                     /locus_tag="ACHJ48_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4708989.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVNVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            complement(5283..5684)
                     /locus_tag="ACHJ48_003508"
     CDS             complement(5283..5684)
                     /locus_tag="ACHJ48_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4708990.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVSALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            complement(5681..6259)
                     /locus_tag="ACHJ48_003509"
     CDS             complement(5681..6259)
                     /locus_tag="ACHJ48_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4708991.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAGSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLAQR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            complement(6271..6546)
                     /locus_tag="ACHJ48_003510"
     CDS             complement(6271..6546)
                     /locus_tag="ACHJ48_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO4708992.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            complement(6539..6862)
                     /locus_tag="ACHJ48_003511"
     CDS             complement(6539..6862)
                     /locus_tag="ACHJ48_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO4708993.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            complement(6949..8976)
                     /locus_tag="ACHJ48_003512"
     CDS             complement(6949..8976)
                     /locus_tag="ACHJ48_003512"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4708994.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLNINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGKPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            complement(8921..9256)
                     /locus_tag="ACHJ48_003513"
     CDS             complement(8921..9256)
                     /locus_tag="ACHJ48_003513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4708995.1"
                     /translation="MASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIR
                     GGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEP
                     QHTDDQSEE"
     gene            complement(9378..10502)
                     /locus_tag="ACHJ48_003514"
     CDS             complement(9378..10502)
                     /locus_tag="ACHJ48_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4708996.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLNFFQYSAQL"
     gene            complement(10430..10642)
                     /locus_tag="ACHJ48_003515"
     CDS             complement(10430..10642)
                     /locus_tag="ACHJ48_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4708997.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(10639..12741)
                     /locus_tag="ACHJ48_003516"
     CDS             complement(10639..12741)
                     /locus_tag="ACHJ48_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4708998.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSSSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPGWASRETWLESVPDSTEPSPSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            complement(12741..13232)
                     /locus_tag="ACHJ48_003517"
     CDS             complement(12741..13232)
                     /locus_tag="ACHJ48_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4708999.1"
                     /translation="MDHELKNLVLNINQLAALSGLHRQTVVARLKNIRPAGGHDKLKL
                     YRLTDILTEFMGLPPPVAEGEMDPHERKAWYQSERERLKFEQETAQLIPASDVRREFA
                     IWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEELQ
                     QEE"
     gene            complement(13907..14059)
                     /locus_tag="ACHJ48_003518"
     CDS             complement(13907..14059)
                     /locus_tag="ACHJ48_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709000.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESWLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(14047..14514)
                     /locus_tag="ACHJ48_003519"
     CDS             complement(14047..14514)
                     /locus_tag="ACHJ48_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4709001.1"
                     /translation="MSRVTAIISALVICIIVCQSWAVNHYRDNAIAYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAKNDALRRKLDNGGRVFVKGKCPVPS
                     SDETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(14511..15008)
                     /gene="rrrD"
                     /locus_tag="ACHJ48_003520"
     CDS             complement(14511..15008)
                     /gene="rrrD"
                     /locus_tag="ACHJ48_003520"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO4709002.1"
                     /translation="MSPSLRKAVAAAIGGGAAAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(15008..15223)
                     /locus_tag="ACHJ48_003521"
     CDS             complement(15008..15223)
                     /locus_tag="ACHJ48_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4709003.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            15366..15764
                     /locus_tag="ACHJ48_003522"
     CDS             15366..15764
                     /locus_tag="ACHJ48_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709004.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(15845..16003)
                     /locus_tag="ACHJ48_003523"
     CDS             complement(15845..16003)
                     /locus_tag="ACHJ48_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO4709005.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(16089..>16247)
                     /locus_tag="ACHJ48_003524"
     CDS             complement(16089..>16247)
                     /locus_tag="ACHJ48_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709006.1"
                     /translation="MINYNDGWYYVLARYDNCLVLSTSFNAGSKRFVIYQSAQDKNLQ
                     VDIVRTRI"
BASE COUNT         3750 a         4806 c         3704 g         3987 t
ORIGIN      
        1 ctgtttccgt tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgccggacc
       61 accgttccgc cgaccagact ctgcaggtct tccgccatcc ccgtgaccat gccgtgcagg
      121 ttagaaaccg tcagtgtcgg acgggcagaa ctgcccttgc cgttcagttc aaatcccgtc
      181 ccctgaatgg ggtatgcctg atactgccgc ccctgccagg taaccggctc acctttttcg
      241 ttctgctcat tacagaaaaa ataacgttca ccaccgacct ctgtcagatc gatttcccag
      301 agcaccgcct gggctgactg agtgaggcgt gtcgtctcat gatgtgtttc ctgtggaata
      361 tcctgcatca gagcctccta tgccacgacc tgttcaaaat ctgccgttat ggttacccac
      421 agcgccccca cgcttgccga ccatttacga caaaccaccc tgatcggttt ccagtcataa
      481 ggtggcgtcc actgaaatgc acggacgcca ccgtgccgtt ccagaaaggc ttttaaagat
      541 gggtgttcac atttacgaac acgtatcgtc acgctgtaag tcgacaactg gttattcagt
      601 cccgccgcac gacgctgttc ataaccatcg cccagcttca ctgtcaccac tttcggctct
      661 gataccacat tcatatccgg gcgcactttc cagtgaaacg tctccattac cgatatgctc
      721 cacttaaccg accaccatca cgggcctgct gttgcataaa gtccgctgcc gcttttttcc
      781 cgaggtcata aaccgccttc agggcagccg gacctatctg cccgttcgtg ccatcgttat
      841 tgatctcgat gttgtactgc ggggaaaaca tcgccatacc tgaaccccca atatccgcca
      901 caacccccag cttaccgtca gcaccacgac gcaatggcag aatggcttca ggtcccgctt
      961 cccccatcac acccgcgcct tttgcaaaag caaaaaacgt cggacggtta accaccgtgc
     1021 cactgtaacg actcaaatca gcagactgat aaatcccccc attggcattc agggaaaggc
     1081 tgctgagatc aaatcccagt acactgccaa tcccttttat cgatttcatc atggttgcct
     1141 gcgccaggat tttcgccata tctgacagca cagaagaggt gaaggatttg aaattgagtt
     1201 tgccggtagt gacaaaagtt gccaggccat ttcccatgtt gctgaaggct gacgagaaca
     1261 cctgttctgc tgtttctgcc gtattatctg catccacggt aaaatcctga aatgcacgca
     1321 ggactccgtt tttccagtta ccctgtacaa cttcaagctg ttgccagtaa cggcgattct
     1381 cattcagttg tcggttcagg ctctccgtca gcgcctgctc ggcctttctg tagtcatccg
     1441 tgttatatgt ccctttctgc tcactatccc gcctcaactg ctccagctgt tgctggtatt
     1501 tctggcgaag actcagttgt acctgatatc gctgtcgctg ctgatcaccc atacccaccg
     1561 tggcgatatc caggtcatgt tgctgacgct gagcgcgctc ttcttcagcc agttgactgg
     1621 tcagctgaat tgtttttttc ttcagctcgt tgagtgccgt ctgtttctga agctcctgct
     1681 gttttacatc cagcagcgtc agtgcctgaa tcagttcatc tttacgggcc agcacactct
     1741 tttcatctgc cgtcagtttt ttcccgtcca ggtcgctgat gcgctgctgc agagccagaa
     1801 gctgtttacg cgcttctgtc atcctttccg tggcaatgcc tgctgactgt ctggcagcag
     1861 caatctgtcc ttccacctgt gcctgttgct gactgtactg cagcaataac cgggtggcct
     1921 catcattacg ggtttcgcgt gtttttttct taccggatgc cagggctttc tcgtaacgtt
     1981 cattttcacg ttgtatcgcc gcatccctga cagcctgatc ggcgtactgc atggcattaa
     2041 tacgcgctat ttcacgctga tgtcgtgctg cttccgtttc attcatccgg ttcagtgcag
     2101 cattttcagc attacggcgt ttctgttgct cctgataatt ccgctctgcc tgctcttttg
     2161 catcctgcaa atccttctgg cgttttttct cctgaagatc gttaagacgc tgctgatcgt
     2221 attcaacctg agaagatgat gccgtccagg ggagtctttt cgcccgcgac actttctcct
     2281 gtaaagcggc aatctgttca tccagcgagt cttcacgacc aatattcatg gccgcatccc
     2341 agaaacgact ccacaaatca gacagatact tcagcgtact gccaagcgca ttgaggttat
     2401 tatcaatatc cgcagtacgc cgaccggttt cctctgccag tgcagacatg gctatccgtg
     2461 cagcatcact ggaccgcccc tgctccccaa ggacgcgtat ctgctcaagc tgagtggcag
     2521 taagaaaatg cagctcattg tccagagcct tcgcggcatt tacaggatca tccttcagcc
     2581 gcttaaactg atttatggta tcgctgaccg actggccaac cgatcgctcc atctgtgcgg
     2641 cagctctcgc caccataccg atatcgtttc cacgaaatgc accactcccc accacctgag
     2701 ccagcgcacc ggctgcagca tgttgcgtga taccattccc ggaaatagca cgactgagcg
     2761 tccacagctg cccggcagtg actccggcat aatgccccgt cagcgacagc tggcggttaa
     2821 attcttcccc ctccttctga ccgtcatacc aggctttacc cagaccatag acagccgcgg
     2881 caataccgcc aataaccgct cctggcatca tgcctttcgg tgacatcagt gtgtctatcc
     2941 acccggcacg gttagccagc gttattccgg atcccctcag cgcacctaaa ttgccgcggg
     3001 ccagttcacc tatcagaacg cctatctcct gacgggctgc tgcacttttc agacccagcg
     3061 aatgcgtggc ttttcctgcc tgctccattt tgcggatata cacttctgca gcactgctta
     3121 cccccagctg ggcagcctta gcacgaagca actcagaaga agaaagattc tggcgggttg
     3181 cctgctcttt aagctgacgg ataaacgcca ctttctgtcg ggtagcctct tcctcagcct
     3241 gtgtaagaac acgggttttc gccgtaacct cagaaatcag cgccagataa tcctgctgac
     3301 caatcccgcc actgtttctg gcctgtcgga tctgttgctg aatacgctgt aattcctgca
     3361 gccccgcacc ggcctgtttc acactgtcaa tctgacgata aaacgcagca gccgctttat
     3421 cctgagcctc cgccagagcc atggcctgcg cctgttcctc gcgcattttc tggctcagtg
     3481 cctccatacg ctggcgggtt tgctccacct cgcgggccat gcgttcatga gcctgtgcgc
     3541 tcttctccac cgtctgcgca tggacggatg cggctgttgc agccgaagaa gccgcctgca
     3601 ttgtctgccg ggcggcctga gtctgacgct ccataaaacg ctgcatacgg gcagaagacc
     3661 gttctgcatc gctggctgca ccattcagaa ggtttttgat acggggaatt tcatttttaa
     3721 actctgccgc atcaatcccc aaatcaatga ccaggttggc tatctggtcc ataacgcaca
     3781 cctccggaaa taccttcccc aagatgcatc agttcttcgt ccgttcgctc cggtatcccg
     3841 ttctcttccg gtaaaaggct gaaatcagcc accgcagcat cactgctacc ggacaccatt
     3901 ctcacgatca atgccttcag cgaggcaaac tgcgcatcca tccacacatc actgaagctc
     3961 tgcatccgga aataatcgcc ccactcacca agctcagtgg ccgacatttc cgacagcatc
     4021 cgccgccagt ctgcccgccg gaactcccgg gcaagccgca tgacaaactg catttcccgc
     4081 gtcaggactt ttccggcgtc agcacctcat gctccaaatc cccggcattc tcaatggctc
     4141 ccataccgct cagcgacaga accatctccg cccccgctcc cagggcatca tacgaccatg
     4201 ttgtaataac ggatgcgcaa agcgtctcaa catcctgaga ctgttccgca ttccacagtg
     4261 agcgggaaac cagccaggca ttgatatcca tccccatccg cagaaaagca atctgtcgtt
     4321 cagcctccgg cagttctccc tcttcggcat caaactttgc cgttcgctgc tgaacaaacg
     4381 ccagatattc aattctctgc agcccggaca gctcactgag caccacggac tgcttttcat
     4441 aattaaacgt gccctgtttc agaaacatca tgttctccac ctgcaaaaaa gccccggata
     4501 accggggcaa atgatgagta tcgtcctgtt aacctgcagc gctgacagtc accgcagcca
     4561 cagccacaaa attcccgtca gcggtcatgc ccacaatgct gacgctgccc tgcttcacgc
     4621 ctttcaccgt ggccacaagt ccgttcaggg tcacagtggc tgtctgtgga tctgtcgaat
     4681 gcacactgat cgctttgtca ctggctccgt caggttttac tgtaaaggtc agcgtggtgg
     4741 ttgctcccac attgacactg gcggatgccg gtgccaccgt cagcccggta acgcccacag
     4801 tttcagtgcc ctcctctgcc agatatggac gccccacacc gctgattttc actgtgcggg
     4861 tcatcacgtc ttttgaggca atggttttac ccagtgagct cagccagccg cggaaaacat
     4921 caacagtgcc gttgggatat ttgatacgaa acgcgcagac ttcaccggag tcgaacaact
     4981 gaaccagttt tttctgcccg ctgtcacccg gacgccaggc cagcgtcgcc gaagtatcac
     5041 caacggattt ctgcccctgg gttgtcgttt tccagtctgc atcttcatca tcgagataag
     5101 tgtcatcttc tgcatcagcg gtcatttcgc caggttgcag atccttcacc atcgcaagac
     5161 gcagccagtc agtgtccgac aaagggttcg caaacgcatc gccgttgccg gtatacatcc
     5221 agaacgtcgt ccccgcacct ttcgtctttg ccagtggatt tggcgtggtc attaccacct
     5281 ccttaattcg tgtacgtgat ctggtacgtg atttccgcca tcgcccaggt ggccatctca
     5341 ttatcacgtt gatagttaaa accgagtggg atcagggtgt cgatgagtcc ggaaagtgcc
     5401 gatacatcat tcagggccgg gaaaatggtg ctctccatcc acatatccag ctctgaatcc
     5461 ggtgcctgtg cccggatgaa gacagcaata tgcagaacag cctgccagtc atcttcatcc
     5521 gtcatttttc cggtgtactg agcatcactc agccacaccg ccacggcagg cagttcctgc
     5581 gcatcaacaa atgccggaag cccgtcaaaa aacgtggcgc tgtctccaca ctgttcccga
     5641 aggcgtgcca gtacgacctg gcggatttgt gtatgtcggt tcatcgggtc agccataacc
     5701 tcagttgttg tttcagtgca taccccagct gtttcggcat ttccgcagca atgatgcggt
     5761 cgcgggcatc ttcaaatgcc tgtgtcagcg gtccggacag cgggattttc accacatcaa
     5821 tggggtaacg atttttgcca tcaatacgcc gcatcacatg ccagcgacca ttcgccagtt
     5881 gctgaataaa cgcatcccgg aaaagatatt tacccacctt cagcacactg ccacggtact
     5941 gcagttttcc accacgctgg gccagtctga cccgggctgt ccccagctta atggcgggca
     6001 gattgccccg gttaacgcgg atcctggccg tcatttttcc tgacggactg gctttaaaca
     6061 cccggacacg ctgacgaacc agtttcaggg ggatcccttt cacctggtta tctcccgcaa
     6121 cggtattccc ggcaacctgc cgggtggcaa ccgagcccgc tttctgtgcc acacggttta
     6181 tcgcccatgc gctggcctgt ggcaccatac gggtatcaag gctgttcaga ttgcggatgg
     6241 cattctcaag ccccttcatc ccacacctct ttactcaata aagatcattg gcttaccgtt
     6301 aaagcgttca tgccgtgtga ccgtccattg ttgtccgtca taaacaacgc gatccccgcg
     6361 ccgtgggcgg tatcccgaag aaaacaccac cagagagacc gcaggtccgg acagagcatt
     6421 cagctctgcc agtgtttctc ccgggatcac agtcatatcg acatcattaa tcgaggctgt
     6481 ctttcccatc tttctgaccg tgatagcatc catacgcgct gccagccggg aaaagggatc
     6541 agacattgag ttttaccggc acttcttctg cactggttcc ggcatctgcc cagacaaccc
     6601 cgaccagcgg atcagagccg ctgttagtca gctgaacttt tcctgacttc agataaacct
     6661 tcttacccgt tttcatgtca tccgttttca gcttaggcag cataaacaca ccttcggtca
     6721 tgccgtcgcc tgtttcaccc tgtggaatat cggtcagcgc caccgcaaaa acatcgccca
     6781 cctgcaccag atctccgctg ctgatggctg cactggcaac aatcgccacc gtttttcctt
     6841 cttctacaaa attctttgcc ataactgtct ccgcacagcc ccgttcaggg gctgatttca
     6901 ggtacaaaaa aagcccttac gggccatcag agttgttgtc tgcgacgttt acgccgtaca
     6961 tttcaccaga ccgcggtgat caactggcgc gacaccggcg tcaatacgca ctttcgttgt
     7021 cacgccatcc acactgaagc cctccatctg atcaatatat ggcgtatcca caccgttgag
     7081 ataagccact tcaatcgtat cggagccttt tgacgcagcc aggtagaagg tggtctggct
     7141 gttatcatca agacgaggct ctgcaataac ggtcgcaaaa tctttcaccg ggttaataat
     7201 accggcgtta atgtcagccc ccttgacact tgaggagcga atgacctggt tagcaacaga
     7261 ctccatcgcc gtcggtacca gtacgaacgc aggacgaata ttcagatgac gctccccctc
     7321 tttctgaacg cgcatcaact ggcgggcttt atccagcgat gccacgtcca ttgcagcgct
     7381 ctccagtacg tttgcatgtt tcgctttatc gaacagactt acattatctg tggagatttt
     7441 cgggttagac gtcagaatgg cataaaccag atcggcaata gtggatttcg ccgcacggcc
     7501 cagtttcatc gggacatcgg tcagcatatt cagatcatca ttgataatgg cctgacgggt
     7561 gatactgaac agctcgccat aggtcgccag tgcaatagtg gcctgtttat ctccggtggt
     7621 gacgtattta tattccgccc cttcacgcac ctgacgcaga gcactgaagc cccccatacc
     7681 cacacgatgg gcaattttaa aatcagacaa ctgacctttc cgcgtccact gttcataggt
     7741 ttcaggggca tcttcccagc cctgcagaat ggctttgttc gcaacatcca gcagaatatt
     7801 accgaagtca gacgtactgt gtgtgaacgc cgcaccgacc atctgcatcg ggttataact
     7861 ggaaacccca ataccccgtt cagtcagtga catacgggca tattcacgca gggtcatccc
     7921 gttgtagaca ttatcacgtt cggttttttc aaatccggca cgcgccatca gcgcctggcg
     7981 gatcccatcc cccacaaaat taccgtttcc ggcataaata tgagccgggg tatttttatt
     8041 agatggcgtg gactcgcgcc ccatctcgtt caacagcttt tcgcgggcct gctccagcga
     8101 acattcagga tcggcaagac actgagcctg cagcgtctga taacgcccgc caaacatggc
     8161 aaacagatca ttaataccgt ttacacgcgc tttttgctct gccagtacct gcgcacggat
     8221 actgttttca tccaccacag gtgctgctgc ctgcactggc gtccgggagg ctgcaggtcc
     8281 atcatcctgt acgcgtggag cactgttgcg tggcggagta atcatgtttc gaatggattc
     8341 cggcatcttt ttaaattcct ctgtacgttt tgactgaata catgccattg ccttaacggc
     8401 tggcgtcacc tgatcagcaa atccatgtgc cagacattcg gcaccggaca tccaggtctc
     8461 atccgccagc atggcagcaa tttcatcggt ggttttcccg gttttctgtg cataagcggg
     8521 taacagaacc gcctcaactt tatcgagcag gtcggcatag gtgcgcatgt cctccgcatc
     8581 accgcccgta aagccaaatg gtttatgaat catcatgaag gagttttccg gcataatgac
     8641 cggttttccc accatcgcaa tgaccgacgc cattgacgcc gccacaccgt cgacataaac
     8701 ggtaatggac gcaccatgtg ttttcagtgc attaaaaatg gcgatgcctt caaagacatc
     8761 gccacccggt gaattgatat tgagattaat gtgggtgata tcgcccagtg cattcagatc
     8821 actgataaac tgcttcgctg taacacccca gaaaccaatc tcgtcataaa tataaatatc
     8881 cgcgtcactc tggtgaccag cctgcatcct gaaccaggaa ttattcttcg gactggtcgt
     8941 cggtgtgctg cggctcctgt cgtttcgttg cggcactgct gcctccttta tcactggccg
     9001 gatcggtatc aaataccaga tccagcttgc ggttttcatc aatttcggcc ttgcgccgac
     9061 gtttgacatc atccggatta cgaccacctg cacgtaccca gtctgattct gtcgctgctc
     9121 caccacgaat ctgaattttc caggcctcag cctccttaac agggtcaatc cacggcatca
     9181 ccggtccgga atacaccgcg gtatacagtg aagaacggtc aagatcgcgg ggtagcctga
     9241 taacaccgga tgccacagcc tgtttcagcc aggcacgata catcgggcgg gtgacggcac
     9301 caataaacca gtcctgcagg atcaggtagc catcagtgga ttcaaccagc tcctgacgct
     9361 gggcgctgta agtgccgtta tagttgcgcg ctgtactgga aaaaattcag acgactgccc
     9421 gccgccacgg cacgcaactg accattacga aaagtttcaa ggttaggatt ggggcgatcc
     9481 gacttcacca ttccgatttc ttcgccgggt ttcagatcgt cgtaaataat gcctggctga
     9541 atggtaagct cgcgttcctt atccttgctg ccattaccat ccggttcata gctctgccca
     9601 tcgcctttcc ggatgtacat ccccagagca gcggcgatcc ttgctgcagt cagctcagaa
     9661 tcctcatact ctttcagggc actgaggcgg atcagcacac cggacaacaa agacgtcccg
     9721 cgcatctggt gcagacggcg aacaaattta agatgcagca ttcgctctgc atccacttct
     9781 ttggtttcca tctgccgtcc ggatacggga cggcttttat acaccagata tttttcggga
     9841 cgcccccagt catcaacaaa cacgccctga ttcagcctgt tgctctcatc actggtcatg
     9901 ggaataaagt ctggctcgag cgcctccagc cagaaatgaa caccggcaga aggcgtcagg
     9961 ctgtttatgc gcccggaaac catctgggca aacacctcac catcgcgcag ccaggtacgc
    10021 agcatcagac gttccagcat cggacgggta aactgcccgg tgacttccgg actgacagac
    10081 cattcactcc atcgggtgcg tatctccgct gccagatcac gggcaatggc cccattgcgt
    10141 aataccggat gtggctcgac aataatcccg tttttcccca ccacccgttc ttccagcttg
    10201 tcaaatacac caataaccag atcgtggttg ttatcaaggt aacgggcctg ctcacgtaac
    10261 gacacggccc cgtactggct taactggtcg gcagttcggt tttcccgccg ggctttgtgt
    10321 gtccgcgtcg tttttacggc ctcataagcc tggatcaccg cacgggaacg cagccttgcc
    10381 gctttccatc ctggtgaaaa aacgccaatc acatcatcaa gaattgccat cagaacctcg
    10441 ccagccggta cccgggatgc ccccgtcgtc gtgtaatcag agccgcaagg cggcgctccc
    10501 actcctgccg tccctgccgg atctcagata agttttccat ggtcatctgc tgaccattaa
    10561 aggtgacgga ttttccgtcc agcaccgcca tttcagcttc cgtataacgc tgaatcatgg
    10621 cttcgatatc attctggttc ataaccatcc tccggaagtc agccaggggt taacatcgtc
    10681 agttactgtt ttcttccgtt tttgtttttt aacaggcgtg gataccggtt ccggtgaggg
    10741 tgacggttcg gtactgtccg ggacactctc cagccaggtt tcccggctcg cccaccccgg
    10801 tgcatccggc cagcggatct tttcgtatcc atgcagaatg accagagcct cggcatacac
    10861 catcaggtca aaagcttcgt tggcaccgcg acccggctta ctccatttcc cgtcactact
    10921 ccgctcttca tacgtcagtt cgtcgtaaaa ccagctcccc agccagtcag ggaaatgcac
    10981 atagccggga cctggagagt cacgccataa cgcgttattc acccggtctt tcagtgcatc
    11041 cgtctgaaga agccagagcg gcacatcacc tgcggcctgc gcccgtcggc ccgttcgtcc
    11101 ggtgttatca gggaatgtac gggtgatcag ttttgcgcgc cggatgctgt cgcccttaaa
    11161 caggtaaata cgtttaccaa ggccatcacg acggcaacga cgccagaatt tataggcatt
    11221 atcagtgacc ccgtcttcac cgccggagtc caccgccatt gccatcagtc gcatttgttg
    11281 agaaggatcg gaggccagcg gccagctttt atgaaaaaca tccgtcagca ggacatccca
    11341 gtcttccgga tagctggccg gatcaattcg ctggctctca ccgtcgctgt caccgcgcaa
    11401 tgactgcgtg atgttgtaac gatcaataat ccagcgttcg ccacggctgc catagcccgt
    11461 tacctgaacc acaaaacggc gatgacgtcc cgcctgcaca tccactgtcg ccacaaggaa
    11521 attaacgcca tccggcacac tgctggaagg aactggctct gcccgctgct caagcagttc
    11581 actttttcgt tgctccatgc tggcgcgggg aagataaggt aatccccagt cggtattgat
    11641 aaccgtcttg agtgtttctt cacttccggt tgtctcgtat tcctgttctg cagtaagcag
    11701 tttgtaaacg agttgcgaga gtgtctggta agcagctgcc ggaccctcca tccagaatga
    11761 cgcaatacgt gagcgtcggg gatcaccata acgactacca tccgcattga tggattcacc
    11821 atcccgcaac cagaccccac gtccgttcag ctcacgtttt tgttcaggca taatccgtcc
    11881 tgaacaggaa ggacactgaa tataagccgc ctcacttgcc agcacgggat cggcaatatc
    11941 acggaaacca gcaaccacat cgccgcaggg ctgaaaatac tcaccacagt gtggacaggg
    12001 ccagtaccag cgacggcgat cgccacggtt atagagcgac agtatccccg tggttggtgg
    12061 agcctcatgc ggtgaagtcc gtcgccattt cacatccttc acatccctgc cgggggaact
    12121 ctccaccagc gtcataccac tggacataaa tgtggtggta cgttttgagg caagagagaa
    12181 ggcatccccc tcgccatcaa tatcttccgg aaaacggtca taatccgtca gcgccacgca
    12241 tttataatct gatgaggaca tgatattgac tgacggccag ccgattttca ggtagttgcc
    12301 agcaaggaat gttctgtcat aaacgttgtt gtcatttttg ttcggactca ggcgactgac
    12361 cacttccggg ctgacgcgaa acgttctggc gagtcgtttt ttggagtgtt cgcgggcttt
    12421 ttcctccgtc atctgaatga tcagcatatc cgcaggatcg caaatcacgt tgtaaatcac
    12481 ccagccgtca atcaggccga tagtcttgcc ggttcgtgcc gggccaacaa atatcactgc
    12541 gtcgtattca cgcgaggcca ggcagttcat cggctcaata acatacggtg ccaccagcgg
    12601 atcccacggg actgagttcc cggcccccat gggcacccgc atatactgag caacggcatc
    12661 agcaacccgc attcgtctcg gtgcgcgaag gatataacct gaatcggttc gtgctgcctt
    12721 tgcggtttcc tgattcagca ttactcctcc tgctgtaatt cctcctcatc atccgcacct
    12781 gcttcggtca cccgcagggc tatctgatcg cgcagatcat caataatgga ctgaacacgg
    12841 ctcacagcgg caggctgcag accacagtca cgttccagaa tatccggtaa tgtctccagc
    12901 acctgcacga ccgcttttgc ccagatggca aactcccgtc tgacatcact ggccggaatg
    12961 agttgtgccg tttcctgttc gaacttaaga cgctcacgtt cagactgata ccaggctttg
    13021 cgttcatgtg gatccatttc gccctcagca accggcggtg gtaaccccat aaattcagtc
    13081 agaatatcgg tcaaccggta tagcttgagt ttgtcatgtc caccagcggg acgaatgttt
    13141 ttcagtcttg ccacgacagt ctggcggtgc agaccagata aagccgccag ttgattaata
    13201 ttcagcacca ggtttttcaa ctcatgatcc atatttcctc cggagagctt taaacatgca
    13261 tcgtgcgaac aactttaaga aaacgcgttc gatgtcgaac aaaaaacact caattcgaca
    13321 tacaaaaaac aaataaccat taataatcaa taagatgcaa agatgatggt ggccgataaa
    13381 aatgcaaaaa ctagcctttt tccgcgacgc tcccgccccg tggcaggcca ccccaccggg
    13441 aggagccgtc agcctgaaag ccatgacgaa cgtctgatac agcccttgca tgaatggcat
    13501 cgggataatc cagaaaggaa tagcatcgac ccacaagaat ctgtgtgagt gtcctgtttc
    13561 ttccaccccc gcacaggact ggcgagcatg agggacaaac ccgcgaatca ttagcgcggt
    13621 aaaaacccgg tgtgcatcgt ttttgattat tcccgcacac tcgcgcagaa ggagttcccc
    13681 gtcgggctac ggtcatggtt aatgcgggaa tacagcgacg atacagcgca tgatgtgtca
    13741 ggcttgaata cctttatccg ttaaaaggga tatcagttaa gttatcccgt gtagggtata
    13801 agccattatc aaagccactc tgtagggagt ggcttttgta atggcaataa aaaagccccg
    13861 cgaatgcgag gctaaatcca ggtattttta atgactggct cttatctcaa cgcagcccct
    13921 taccgcgcgc cagatgctca atatcaagca tcagcaatga gatgtttaac catgattcac
    13981 tccagaagtg agcaccaccc tgtctacaga gccagatgtg aaggatgatg agtaaaatta
    14041 tcgctatcat cgaaggcatt gcgtcctgat gtattcctga agcgttctca gtgctgtttg
    14101 gtcgcggata attccgtccc ggataccgag aacgtttcgt ccagcaactg gagagagttc
    14161 gacggtggca tcattgccca tgccggaggc gctggaggtt tcgtctgagg atggcacagg
    14221 gcattttcct ttgacgaaca cccgaccacc attatcaagc ttgcgccgaa gagcatcatt
    14281 tttagctttc gcatcagcta actccttcgt gtatttagca tcgagtgcat cagcagcacg
    14341 ctggcgctgc tgcatgtcag taatggtggc ggtcgcctgc ttcagctcac tgactttttt
    14401 atcacgctgt tctttatagg cgatggcgtt atcacggtaa tgattaaccg cccatgactg
    14461 gcagacgatg atgcagataa ccagagcgga gataatcgcg gttactctgc tcattgttgc
    14521 ccccacaaac agacttcacg ctcaatctcg cggcgagtca tcagcccttt ccattgctta
    14581 ccgccagcgt atgtccagcg ccgtagctga tcacatgcgc ctttgatatc accctggttt
    14641 attttgcgaa gaagcgtcga tgttctgaaa ttaccagcgc ccacgttgta aacgaacgag
    14701 taaagagcgc cgcgcgttgt ttccggtata tcgactttga tgtacgggtt aatttgtctg
    14761 gcgaccgtgg caaggtcttt attcaggagg gctttgcatt ctgcttcggt atacgtttta
    14821 ccgggaatga tgtcttttcc ggtgtgtccg tgacatacag tccatacgcc aacgatatct
    14881 tcgtatggta tgtagctgac accttccagg ccatcgtcac cactcggacc agtgatgagc
    14941 acagacgcta tggcagcagc cccaccacca atagcagcag caacagcctt gcgtaatgat
    15001 ggcgacatta ttcacctctc gcagccttac gcttatcttc tttaatcttg aaataaaggt
    15061 ttgtcagata cgtcagcaag ccaaacacca agctacccaa tacgcctatt gccacccact
    15121 gggatgggga gactttgtcc agcaactgca gtagccagta tcccgtcccc accgctgacg
    15181 tggtgtatga cacacctgtt gttatttttt ccatctggta cataccccgt ctcccgttat
    15241 ccggaagctg acaacaataa aaagccacca gttaattcct gatggccctg atgcataaac
    15301 gtcataatac ctgactgtta tgattgacaa taatgataat gtttatatag aaaggttccc
    15361 gatgtgtgtt acatatcatt tctccacggg gaatatcccc acgccagcgc agactctttt
    15421 acccgttctc ttctgcgctg gctctttttt attatgctgc tgcatttacc tctggcacca
    15481 tgctttctat ctcaacacaa tacgtggtac ttcttgtaac caatatcata acgattaatc
    15541 gacatagaat ttctcccgtg tacaggaaca gagttaaaaa gccggaaccg gaatcaaatc
    15601 acaggatgac catctgccag tggcaggtca taaaaaaaag gccgcgccat gcgcagccag
    15661 aactcacaag gaaaatgata gaaggaaata acattagtga tgtacgcatg gcgcctcccg
    15721 ctaagttctg caatgatcaa acagaactcg ctacgtgccc ttaaaactcg atcatttagc
    15781 ccctccaagg aggattcacc atgcggttga ttttttaata aacagtaaac aaaaaagtca
    15841 agaattattc attctgttct ttcatcatcg gccacagcaa taccacaatg ccgcagacca
    15901 gagcgccatc agtcagtacc aacattatcc tgctggtgaa atccatcatc accatcacta
    15961 aaagcaggat cacaacagca agcagacaca gtttataaaa caatgttcag gaaacgcatt
    16021 cagcatgcct aaggttctat tcctacgaat agccaacttg caacttaaaa tattatttat
    16081 gcagccaatt aaattctggt ccttacaata tcaacctgaa gattcttatc ttgtgctgat
    16141 tgataaatca caaacctttt actacctgca ttgaaagaag tagacaaaac cagacaatta
    16201 tcataacgag caagaacata ataccaacca tcattataat taatcat
//
LOCUS       DAZFDC010000105        16225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFDC010000105 DAZFDC010000000
VERSION     DAZFDC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.105"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(286..711)
                     /gene="arsC"
                     /locus_tag="ACHJ48_003525"
     CDS             complement(286..711)
                     /gene="arsC"
                     /locus_tag="ACHJ48_003525"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO4709007.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            complement(724..2013)
                     /gene="arsB"
                     /locus_tag="ACHJ48_003526"
     CDS             complement(724..2013)
                     /gene="arsB"
                     /locus_tag="ACHJ48_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO4709008.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2067..2420)
                     /gene="arsR"
                     /locus_tag="ACHJ48_003527"
     CDS             complement(2067..2420)
                     /gene="arsR"
                     /locus_tag="ACHJ48_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO4709009.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSVDSKNTCS"
     gene            3164..3247
                     /gene="dinQ"
                     /locus_tag="ACHJ48_003528"
     CDS             3164..3247
                     /gene="dinQ"
                     /locus_tag="ACHJ48_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO4709010.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3301..4653)
                     /gene="gorA"
                     /locus_tag="ACHJ48_003529"
     CDS             complement(3301..4653)
                     /gene="gorA"
                     /locus_tag="ACHJ48_003529"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO4709011.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4725..5567)
                     /gene="rlmJ"
                     /locus_tag="ACHJ48_003530"
     CDS             complement(4725..5567)
                     /gene="rlmJ"
                     /locus_tag="ACHJ48_003530"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO4709012.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEPYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5770..7812
                     /gene="prlC"
                     /locus_tag="ACHJ48_003531"
     CDS             5770..7812
                     /gene="prlC"
                     /locus_tag="ACHJ48_003531"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO4709013.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQVLREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7820..8572
                     /gene="rsmJ"
                     /locus_tag="ACHJ48_003532"
     CDS             7820..8572
                     /gene="rsmJ"
                     /locus_tag="ACHJ48_003532"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO4709014.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8621..10091)
                     /gene="dtpB"
                     /locus_tag="ACHJ48_003533"
                     /pseudo
     CDS             complement(8621..10091)
                     /gene="dtpB"
                     /locus_tag="ACHJ48_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            10257..10310
                     /locus_tag="ACHJ48_003534"
     CDS             10257..10310
                     /locus_tag="ACHJ48_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709015.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            10288..10341
                     /locus_tag="ACHJ48_003535"
     CDS             10288..10341
                     /locus_tag="ACHJ48_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709016.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(10408..10842)
                     /gene="uspA"
                     /locus_tag="ACHJ48_003536"
     CDS             complement(10408..10842)
                     /gene="uspA"
                     /locus_tag="ACHJ48_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO4709017.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11233..11568
                     /gene="uspB"
                     /locus_tag="ACHJ48_003537"
     CDS             11233..11568
                     /gene="uspB"
                     /locus_tag="ACHJ48_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO4709018.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11639..13138)
                     /gene="pitA"
                     /locus_tag="ACHJ48_003538"
     CDS             complement(11639..13138)
                     /gene="pitA"
                     /locus_tag="ACHJ48_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO4709019.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13370..14572
                     /gene="yhiN"
                     /locus_tag="ACHJ48_003539"
     CDS             13370..14572
                     /gene="yhiN"
                     /locus_tag="ACHJ48_003539"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO4709020.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(14890..15942)
                     /gene="yhiM"
                     /locus_tag="ACHJ48_003540"
     CDS             complement(14890..15942)
                     /gene="yhiM"
                     /locus_tag="ACHJ48_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO4709021.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4181 a         4097 c         4211 g         3736 t
ORIGIN      
        1 gcactgataa atcataatcc agtacgtttt taaactgctg cactgtatcg ctgatcacta
       61 agcagctcac tgatacatca tgctggacgc gataattgga atctcacgca gggagtaatg
      121 tgtaaatagc aaaatcgcct gatgcgctac gcctgtcagg cctacaaata acatacaatt
      181 tattgaattt tcatgacttt gtagatcgga taagacgcgt tagcgtcgca tccgacataa
      241 ataaagcgca cttttctaac aacctgtggg ggggatatag ccgctttatt tcaggcgctt
      301 acccgcttca tcaaccactt tctcgccatc ttccttggtg aatgcgcctt tttgcgcatc
      361 tggcagaatt tccagcacca cttctgaagg gcggcacagg cgagttccca gcgtcgtcac
      421 cacaatcggg cgattaatca gaatcgggtg ctgaagcata aagtcgatta accgatcgtc
      481 agtaaattta tcttccgcaa ggcccagctc ctcatacggt tcgacgtttt tacgcagcag
      541 cgcgcgtacg gaaatcccca tatcggcaat gagtttgacc agttcatcgc gcgttggcgg
      601 agtttccaga taatggataa tggtcggttc tgtgccgctg ttgcggatca tctccagcgt
      661 attacgcgac gtgccgcagg ccgggttgtg ataaatggta atgttgctca tatcagtatc
      721 tcattacaaa gtgaaagaga gacgtagcgc cagcgcagcc agcgtcacaa acagcacagg
      781 cagggtcatg ataatccctg tacggaagta atatccccag ctgatagtca tattcttctg
      841 cgaaagtacg tgcagccaga gcagcgttgc caggctacca atcggggtga ttttcggccc
      901 caaatcgcag ccaatcacgt tggcataaac catcgcttct ttgataacgc cagatgccgt
      961 gctgccatca atggacaacg cgccaaccag taccgtcggc atattgttca taatagaaga
     1021 gaggaaggcg gtgaggaatc cggtgccaag cgtcgcggcc cacaggccgt tatccgccag
     1081 cacgttgagt acgccagaaa gatattccgt taatccggca ttgcgcaggc cataaaccac
     1141 cagatacatg ccgagcgaga agatgacaat ctgccagggc gcaccgcgca gcactttacc
     1201 cgtattaatc gcatgaccgc gtttagcgac gacaaataat atcaacgcgc ccactgccgc
     1261 aatggcgctg accggaatgc cgagcggttc caggacgaaa aatcccacca gcagaagcag
     1321 taaaacaacc cagccagttt tgaacgtagc aggatcttta atcgcttctg cgggagattt
     1381 cagcagcgcc atgtcgtagt tctgcggaat atctttgcgg aaatagagat gcaacatcac
     1441 cagcgtggca acaatcgcgg cgatatccac cggcaccatc accgaggcat attcgcgaaa
     1501 gccgaggcca aagaaatctg cggaaacgat attcaccagg ttggagacaa taagcggcag
     1561 gctggcggta tcggcaatga atccggccgc catcacaaac gccagcgtag tgcctttact
     1621 gaaccctaaa gccagcagca tggcgatgac aatcggtgtc aaaataagcg ccgcgccatc
     1681 attggcaaac agggcggcaa cggcagcacc gagcaggaca atccaggtaa acagcaagcg
     1741 accgcgtcca ttgccccagc gtgaaacgtg cagtgccgcc cattcaaaaa agccggactc
     1801 atccagcagc aggctgatga taataacggc aataaacgca gccgtcgcgt tccagacgat
     1861 attccacacc accggaatat cgcccggatg tactacgccc gtaactaacg ccagtactgc
     1921 gccgagcgtt gcactccagc cgatgcctaa acctttcggc tgccagataa ccaatacaat
     1981 ggtcaggaca aagatagcgc ctgccagtaa cataatgcct cccggaaaaa cacatgtgca
     2041 aatccatatg tgtttagata aattttttaa ctacaagtgt tcttactgtc tacggaacaa
     2101 ttttgtctgg ccaggttgcg gacaatcacc tgaacctttt cctgttcgca tcgccaggcc
     2161 tgctcaataa ttttcgcggc ccatgatgga atatgcggtg ataagcggta atgaacccac
     2221 ttaccctgct tgcgatccag caacaaaccg ctttcacgaa gaagcgccag atggcgggag
     2281 atcttgggct gcgactggtc gagagcagtg cagagatcac agacgcataa ctctcccagc
     2341 tcgctgagca gtaaaacgat gcccagacgg gtttcatcgg caagaatttt gaacagttgg
     2401 atgggtaaca gaaatgacat attgcgctcc tgattgttgc aggtagtgtc tctcttcgaa
     2461 gcggataagt caaaaacata tatggtttaa cgaatgtgta tgtgcagagg aaggtaatag
     2521 gtgtgaatta agcgttggct attcatttga aaggaggtct gaatcaggac gtaaaaaaac
     2581 cgactttgcg tcggtttttt actttccagc ccagagttgg tggctctggc tggagtgaga
     2641 atgcccacat ctaacggttc aatactatta gccagtggct aacattgcaa gcgttagcga
     2701 tttcttcagc tggagaaggg acagtttgct atacatgttg gtgaaagagg atttatatac
     2761 tttcatcggg tcgctgccgt accaggcttt gtccgaatcc ggatgtaaaa aaaccgactt
     2821 cgcgtcggtt tttttactct tcagccctga gttggtggct ctggctggag tgagaatgcc
     2881 atcatctaac gcatcaatac taataccata ctgaaactat tgcaaggaca tgctggtttt
     2941 ataacctgaa tatactgtat gattatccag ttagctctga ggcattttca ctctggcaat
     3001 gcgcataaac gctttcaaag tcctgttcag tggtacgggt ggtgccgtta actgatgctc
     3061 tggccggagt gagaatacca ttatctaaca atagggcatg cgccgtgaca ggcgatggat
     3121 gagtaagcgg atgcattctc actccatcgt atggagaaca cgggtgattg ataaagcaat
     3181 catcgttctt ggggcgttaa ttgcgctgct ggaactgatc cgctttctgc ttcagcttct
     3241 gaactgatag cggaaacgta attaagggca aagagcacac cactctttgc cctttaacat
     3301 ttaacgcatg gtcacgaact cttctgccgc cgttgggtga atggcgacgg tattatcgaa
     3361 gtcttttttg gttgcgccca tcttcagtgc caccgcgaag ccctgcaaca tttcgtccat
     3421 accaaaacca atgccgtgaa taccgacaat cttctcttct ggcccaacgc acaccagctt
     3481 catgcggcac ggctggcggt gagtggtgac ggcggtatac atcgcggtga aagaggattt
     3541 atacactttc acctgatcgt cgccgtactg ctcgcgcgcc tgcggttccg ttaaaccaac
     3601 agtaccaatc ggcggatggc tgaagaccac ggtcggaatg ttgctgtaat ccagatgctc
     3661 atccggcttg ttattaaaca ggcgttcaga gagacggcga cccgctgcaa ctgccaccgg
     3721 tgtcagttct actgcacccg tgttatcgcc caccgcgtaa ataccttcaa cgttggtgtt
     3781 ttgatattta tcgacgacga tatagccttt ttcgttggtt ttaacgccag cggcttcgag
     3841 gttgatgttg tcattggcag gctcgcggcc aatcgcccaa atcaggcaat ccaccgtttc
     3901 actgcgacca tcttccagct ccagcgtcag gctaccatcg gcatttttca ctaccgcttt
     3961 cgggatggcg ttggtgtgca actgcgggcc ttcggcgttc atcacttcga ccagcgtttc
     4021 agagatcatc gggtcgaagc tgcgcagcgg cgcatgttta cgcacaaaca gatgcgtttt
     4081 cgcgccgagg ccgttaatca cgccagccag ctcaacggcg atgtaacccg cgccaacaac
     4141 cgccacacgc tctggcaaag caggaagggc aaagaaacca tcagaatcaa taccgtattc
     4201 cacgcccgga atatccgggt ggctcggacg accgccggtg gcgatcagaa tatgatcggc
     4261 tgtgatggtt tcaccgttaa cttccagcgt tttggcatca acgaagcggg caaagccttt
     4321 gattacatca acgttatttt taccgagcac gttttcatag gaagtatgaa tacggtcgat
     4381 ataggcggta cggctggcga tcaacgtttc ccagttgaat ttattgatag tggtatcaaa
     4441 accataatcc gggccgtaca tatggatcgc ttcacggatt tgcgccgcgt gccacatcac
     4501 tttcttcggt acgcagccga cgttcacgca ggtgccgccc agctctttgg cttcaatcag
     4561 cgcgcatttc tgaccgtaca tagccgcgcg gttgatggag gcgataccgc cgctgccgcc
     4621 gccgatggcg atgtaatcat agtgtttagt catgacagag tgtccttatc gttgattacc
     4681 gcgattgtag cgcgagacgt aataggtgcc agcaatggct gcaattactc tggcacgatc
     4741 cagcttacgg tggcgtgccc ggtgccagct ggaaccagtt tgctgtgcag ccacggcagc
     4801 acgttattca tctgctgttc cagtttccac ggcgggttaa tcacaatcat gccggaagcg
     4861 gtcatgccac ggcgatcgct gtctggcagt accgccagtt caatttgcag aattttgcga
     4921 atgccggtcg cttccaggtc gtggatcatg cgcttaattt gctggcgcag caccaccgga
     4981 taccacaatg cgtaagtgcc agtggcgaaa cgtttgtaac cttctgctat cccgctgact
     5041 accgcctgat agtcggtttt catttcatac ggcggatcga taaggattaa accacggcga
     5101 gaaaccggcg gcagtttggc cttaagctgc tggaaaccgt cggatttttc gacgcgcgca
     5161 cggctgtctt tctgaaattc agaacgcagc aacgggtaat cgctcgggtg cagttcggtc
     5221 agttgcaggc tgtcctgttc acgcagtaac tgacgggcaa tcaacggtga accggggtag
     5281 taacgcaact gaccgctacg gttgaagtgt tttaccacat tgatgtacgg ctccagctcc
     5341 gcaggcaaat cgtcctgctg ccagatacgg gcgatacctt cgagatattc gccggtacgc
     5401 tcggcatgtt cgctgcctaa ctgataacgc ccggcccctg cgtgggtgtc gagatagaga
     5461 aacggtttat ctttctcttt cagcgactcg atgatcaggc tctgaacggt atgtttaagg
     5521 acgtcggcgt ggttgccagc gtgaaagctg tggcgataac tgagcatggg taaaggtgtt
     5581 ccggtaagta aaaatcggcc cgtattcggg gcgcacaaaa gcctatcagg acagtatacc
     5641 gaaaagaggc cgccgccgcg aaagcgtaac gtttctcatt gaaattcact acacttaacc
     5701 ccatgctaca aacattatgt aaagcgcctg ttgagcgctt ccttaacctc tttaaccagg
     5761 actgcgctaa tgacgaatcc gttactgact ccctttgaat tgcctccgtt ttctaaaatt
     5821 ctcccggaac atgtcgttcc agccgtgact aaggcattga atgactgccg cgaaaatgtg
     5881 gagcgcgtag tagcgcaagg agcaccgtac acctgggaaa atctctgcca gccgttggcg
     5941 gaagtggacg atgtgttggg gcgtattttc tccccggtca gccacctgaa ctcggtgaaa
     6001 aatagcccgg aattgcgcga agcgtacgaa caaactctgc cgctgctgtc ggaatacagc
     6061 acctgggtag ggcaacatga agggctgtat aaagcgtacc gcgacctgcg cgatggcgat
     6121 cattacgcca cgctgaacac ggctcagaaa aaagcggttg ataacgcact gcgcgatttt
     6181 gaactctcag gcatcggtct gccgaaagag aaacaacagc gttacggcga aattgccact
     6241 cgtctttctg aactgggcaa ccagtacagc aacaacgtcc ttgatgcgac gatgggctgg
     6301 actaaactcg ttaccgacga agcggaactg gcggggatgc cagaaagcgc gctggctgcg
     6361 gcaaaagccc aggccgaagc gaaagagctg gaaggctacc tgttgacgct ggatatccca
     6421 agctacttgc cggtaatgac ctactgcgac aaccaggtct tgcgtgaaga gatgtatcgt
     6481 gcttacagca cccgcgcttc agatcaaggt ccaaacgccg gtaaatggga taacagtaag
     6541 gtgatggaag agatcctcgc gctgcgtcac gaactggcgc aactgctggg ctttgaaaac
     6601 tacgcgttta aatcactggc taccaaaatg gcggaaaacc cgcagcaggt gcttgatttc
     6661 ttaaccgatc tggcaaaacg cgcgcgtccg cagggcgaaa aagagctggc gcaactgcgt
     6721 gccttcgcca aagccgaatt tggcgtggat gagttgcaac cgtgggatat cgcatactac
     6781 agcgaaaaac aaaaacagca cctctacagc atcagcgacg aacaactgcg tccgtacttc
     6841 ccggaaaaca aagcggttaa cggcttgttt gaagtggtaa aacgtattta cggcatcacc
     6901 gctaaagagc gtaaagatgt tgatgtctgg cacccggatg tacgtttctt cgaactgtat
     6961 gacgagaaca acgaactgcg cggcagtttc tacctcgacc tgtatgcccg tgaaaacaag
     7021 cgcggcgggg cgtggatgga tgactgcgta ggccagatgc gtaaagccga tggttcgctg
     7081 caaaaaccgg tcgcgtatct gacctgtaac ttcaaccgcc cggtgaatgg taaaccggcg
     7141 ctgttcactc acgacgaagt gatcactctg ttccacgagt tcggtcacgg cctgcaccat
     7201 atgctgaccc gcatcgaaac cgccggtgtt tccggtatca gcggtgtgcc gtgggatgcg
     7261 gtcgaactgc cgagtcagtt tatggaaaac tggtgctggg agccggaggc gctggcgttt
     7321 atctctggtc actacgaaac cggcgaaccg ctgccgaaag agttgctgga taaaatgctg
     7381 gcggcgaaga actaccaggc ggcgctgttt attctgcgcc agctggagtt cggcctgttc
     7441 gatttccgcc tccatgccga gttccgcccg gatcagggag cgaaaatcct cgaaactctg
     7501 gcagaaatca agaaactggt tgccgtagta ccgtctccat cctggggccg tttcccgcac
     7561 gctttcagcc atattttcgc cggtggttat gccgcaggtt actacagcta cctgtgggct
     7621 gacgtactgg cggcagatgc tttctcgcgc tttgaggaag agggcatttt caaccgtgaa
     7681 accgggcagt cgttcctcga caacattctg agccgtggcg gttcagaaga gccgatggat
     7741 ctgttcaaac gcttccgtgg tcgtgaaccg cagctggatg cgatgctgga gcattacggc
     7801 attaagggct gatcattcag tgaaaatctg cttaattgat gaaacaggca ccggagacgg
     7861 tgccttatct gttctggcgg cccgctgggg gctggagcac gatgaagaca acctgatggc
     7921 gctggtgtta acgccggaac atctggaatt gcgcaagcgt gatgagccaa aacttggcgg
     7981 catctttgtt gattttgttg gcggagcgat ggcgcaccga cgcaaattcg gcggtggtcg
     8041 cggtgaggcg gtggcgaaag cggtgggcat taaaggcgat tatttgccgg atgtggtgga
     8101 cgctaccgca gggctggggc gcgatgcctt tgtgctggct tcggtcggct gccgcgtgcg
     8161 gatgctggag cgtaatccag tcgttgccgc gctactcgac gacggcctgg cgcgtggata
     8221 tgcggatgcg gaaatcggcg gctggttgca ggagcgtttg cagttaattc acgcctccag
     8281 cctgacggcg ctgaccgata ttaccccgcg tccgcaggtg gtctatctcg acccgatgtt
     8341 cccgcataag cagaaaagcg cgctggtgaa aaaagagatg cgcgtctttc agtcgctggt
     8401 gggaccggat cttgatgccg atggattact ggagcctgca cgcttgctgg cgaccaaacg
     8461 cgtggtggta aagcgcccgg actacgcgcc accgctagcg aatgtcgcca cgccaaacgc
     8521 ggttgtgact aaagggcatc gctttgatat ctatgcaggt acgccagtgt aaaagtaaag
     8581 cagggtagcg ataacgctac cctgctttct gcaagaataa ttaatggctt tccggcgtcg
     8641 caatcatgcg tttcagccac ggcaccatca acagcatcac tactgcaacg cccagcgtga
     8701 ccagaccaat cttaccaaac acgttggtat agacaggcaa cgtctcaagc ggatcggtaa
     8761 tgttgtctgg tactgcggta aatgttgcca catagccgcc cagcaagaac gcggcagcct
     8821 gtgtcaggaa ccacatcccg agaataaagc ccatcaaatg ctgcggcacc agagcagcaa
     8881 tcatcgccag gccaagggcg ctaataaaca gttcacctaa gctctggaat aagtacacca
     8941 gcacgataaa ccatggcgat gtcagccctt gtgcatccgc aaaccacatt cccgcagctg
     9001 ccgccgtcaa aaagcccagt gagcacataa acatgccgag agtaaatttc atcggcatcg
     9061 agaggtcttt gcctttgtta cccagatgcg tgtaaatgcc tgccagtatt gggctggcga
     9121 gtaccaccca gaacgggtta agcgcctgga agctgaccgg gttgatggaa aaaccgagaa
     9181 tttcatgatg cacgttgttg atggcaaaga agttcagcga tgttggcatc tgggcgtaga
     9241 gaatgtaaaa caccaccgct tcgagcatca ggacaaaggc gacaaacatt ttattgcgcc
     9301 cggttttatc cagcttgaat gcctgacgaa agaagatgat ggtgacgacg atggagagaa
     9361 caatcagcac cagattggcg acttctacgt tgtgcatcag ccaggcgcag acgaagattc
     9421 atcaccacgc tgccaagtaa tacgtacaac agtttgctga agctcattgg ccggaagtcg
     9481 ggctcagaac caatgtcttt caccattcca cgacaggcga tgtaaaccag taatgcgata
     9541 attaaccccg caccgcacag gttgtaggtg actgaatagc cgaatttatc agcgatcaca
     9601 ggggccagcg ataacgctat caacgagccg atattgatcg acatatagaa cagggtgaat
     9661 gtgccatcaa ggcgcggatc tttcggcgga tagcacttcg aaagcaagct ggctgggtta
     9721 gctttaaaca ggccgttacc gacagcgata gtccccaggg cgatgaaaat caggtcaggc
     9781 ttaagtagcg acatgccggt catgaagtag ccaatcgcca gcacaagtgc tccaagaacg
     9841 atggtgcgtt tggtccccag caggtggtcg ccgacatagc cgccaatgga aatgaggcca
     9901 tagaccagtg cagcaaaagc accaaaagtg acaaaagcct gctcttgcga gaatccaagc
     9961 tgtttaacga agaaaaccgc cagtacgccc tgcacgccgt agtagccgaa tcgctcccat
    10021 aactcgacaa aaaagatcat gaaaaatggg cgaggttgct gcagcatccc catgggtgtt
    10081 gttgtattca tatttattaa ccttccaata ccatcctgaa aagtatgaac gcgtaaggga
    10141 taacgcgcga aaaagtttac atctgtcaga gattcttaat attgatgcgg tgaatagatt
    10201 aatacactat tgcaacagaa aataaccagc tgatcacact aatgatggaa aatatgatgg
    10261 tgttaaatat tgtattggct ggggattttg attgcgaacg cagaccgtag gccagataag
    10321 gcgtttacgc tgatcaggta acaaggcgcg acaaaaagca aaacgcccgc atatgcgggc
    10381 gttcaggaac acgtcgtgga gggaagatta ttcttcttcg tcgcgcagcg gaacaatcag
    10441 catatcaacg tgaacggtgt tgatcagctg acgtgcggaa gacatcagtt tgctccagaa
    10501 gtcctggtgg tgaccacaaa ccactagatc catatcgtat ttcttgattg catcgaccag
    10561 aacctggccc aggtcgccgc tgccgctcag ggtttcagtg attgggtagc ctgcattagt
    10621 ggaaagctcg gtcagcgcat gatgtgtctc ttcagagatg cgtttctgca tatcaccaag
    10681 attcacatca ataagcccgg tatataggtc agagtagttt acatctacgt ggatcagaga
    10741 aactttcgca ttgtatgggc gagccataga gactgctttc tctaccagaa ctttgctttc
    10801 cggggagaga tcgaccgcga tgagaatgtg tttataagcc atagtgttac tccttccata
    10861 aagttgtcga tgactggcca gctagcgttt cttgtgccgt tacgtcaccc gcgtcctgcg
    10921 aactacgtgc gcggggctaa cccagccgta aagattcagt gttaagacca tccttacctt
    10981 atagcgaccc ggatgatccg tcaatccgcc ttgcttaccc gttagataaa caaattttcg
    11041 acatgaatat attgatagtg gttaaccttc tggaaaaaaa acaacctgat ctcctacact
    11101 atctatagag ccgctcggat gttggtcacc acaaaagcgg tttctccagg acctggtgtt
    11161 aactgattgg cagcgtatcg ggagcggtag ccccggggtg gaactccgcg ggcaggtcgc
    11221 cggggaggag gtatgataag caccgtcgca ttattttggg ctttatgtgt cgtttgcatt
    11281 gttaacatgg cgcgctattt ctcatcacta cgcgcgttgt tagtggtact gcgtaactgc
    11341 gatccattgc tctatcaata tgttgatgga gggggctttt ttacctcaca tggccaaccc
    11401 aacaaacagg tgcgtctcgt ttggtatatc tatgcccaac gttatcgcga tcatcacgat
    11461 gatgagttta ttcgccgctg tgagcgggtg cgtcggcagt ttattctgac cagcgcattg
    11521 tgtggtctgg tggtggtcag cctgattgcc ttgatgattt ggcattaagc acttcagcaa
    11581 taataacgca taaaaaaagc gggccagtaa actgacccgc tcgttttggt gcgtactatt
    11641 acaggaactg caaggagagc cagtaaagcc cgccggaaag cagtaccgca gccggaaggg
    11701 taaacaccca ggccatcaga atgctggtca cggttttacg ctgtaagccg ccaccatcta
    11761 ccaccatcgt ccccgcgaca gaagaggaga gtacgtgagt agtggaaacc ggcatcccgg
    11821 tataactcgc caggccgata gacactgccg cagtcatctg ggcagacatc ccctgagcgt
    11881 aggtcatgcc tttcttaccg attttctcac cgatagtcgt tgccacacga cgccagccaa
    11941 tcatcgtacc gatacctaac gccagcgcga ccgccatgat gatccacacc ggtgcatact
    12001 cgatggtgct aagcatgtcg gacttcagtt tcttcaacag gcgctgatcg tcagcactca
    12061 cgccaggcat cttcaccact ttgtcgatag tgtcagaaac gcacagcata atgcggcgca
    12121 tctggctacg ttgatcaagc gacagcttgt cgtagctttc cacatcggtg gtcaacatac
    12181 ctttcaggcg gttgagcgcg ttaatggtat tcgacggatg gcagtggaac tccgcaggtt
    12241 gcgttgcgcc agcttccgga gccggtacta actgatcagc accagtagct tgtttcagca
    12301 gcgcaggatg ctgctcaaag taagcttcga cgttgttgat ggcatcacgg gtacgggtga
    12361 tttcgtagcc agtggcattc atgttcacca cgaagcctgc tggtgcgaca ccaatcaata
    12421 ccaacataac cagaccaatg cctttctgac catcgtttgc gccgtgcgaa aacgccacgc
    12481 cgatagcgga aaggatcagc gcgatacgcg tccagaacgg cggctttttc ttgccgtctt
    12541 tcttttcacg ctccgctggg gtcaggtgga tacgggcgcg tttcttggtg ccgctccagt
    12601 agcgacgcag caagaaaatc agaccgccag caaacaccag gccgacaata ggggaaacga
    12661 tcagagaacc gaaaatactt aatactttcg ggatattgag tgcatccacc actgacgtcc
    12721 cggtcatcaa cgcattggtt aaaccaatcc cgatgatcgc gccaatcagc gtatgagagc
    12781 tggatgcagg caaaccaaag taccaggtac ccaggttcca gataatcgcc gccagcaaca
    12841 tagagaacac catggcaagg ccatgagacg atcccatatt aagcagcaga tccgtcggca
    12901 gcatatgcac aatggcatag gcaacactca gaccacccag caaaacaccc aaaaagttga
    12961 acaccgctgc cataaccacg gcgagctgag aacgcatcgc gcgggtatag ataacggttg
    13021 ccacggcgtt ggctgtgtca tggaaaccat tgatggcttc gtagaacagc acaaaagcca
    13081 gtgcaagcaa taataacagc ccggtatgca aatccaggcc agcaaacaaa tgtagcatag
    13141 gacgttacgc cattttgagg acatgaacgc ggcgcattat cagtgacttt cgcggcgcgg
    13201 gcaaagtgaa atatagactt tttttgattt gcctcctgta tggatttcac tcaaaaaata
    13261 attatcttat ataattcagg caaatacttc cttttagtaa tattgatgct ggtgcgacca
    13321 ctgaggaatc tttacaattc acgcccgttt tttctaagag gagcgcaacg tggaaaggtt
    13381 tgatgccatt attataggcg ctggtgcggc gggtatgttc tgttctgcgc tggcaggtca
    13441 ggcaggacgc cgggttctgc tgatcgataa tggtaaaaaa ccagggcgca aaatccttat
    13501 gtctggcggt gggcgctgca actttaccaa cctttatgtc gaaccgggcg cttatctgag
    13561 ccagaatccg catttttgta agtctgcact cgcgcgtttt acccagtggg atttcattga
    13621 tctggtcaat aaacacggca tcgcctggca cgagaaaacg ttagggcaac tcttctgcga
    13681 tgactccgcg cagcagattg tcgacatgct ggtggatgag tgcgagaagg gcaatgtgac
    13741 cgtcagattg cgtagcgaag tgctgagtgt ggcgaaggat gatacaggct tcacgcttga
    13801 actgaacggc atgactgtcg gttgtgaaaa gctggtcatc gcgaccggcg ggctgtcaat
    13861 gccgggcctg ggcgcgtcgc cgtttggtta taagattgcc gaacaatttg gcctcaacgt
    13921 gctgccgacc cgcgcgggtc tggtgccatt cactctgcat aaaccgttgc tcgaagagtt
    13981 acaggtgctg gcgggcgtgg cggtgccttc cgtgattacc gctgaaaacg gcaccgtttt
    14041 ccgtgagaac ttactcttca cccatcgcgg cttgtctgga ccggcggtgt tgcaaatttc
    14101 cagctactgg caaccggggg aatttgtcag tatcaatctg ctaccggatg tggacctcga
    14161 aaccttcctg aatgagcagc gtaacgcaca tccgaatcag agcctgaaaa acacactggc
    14221 ggttcatcta ccgaagcggt tggttgaacg cttacagcaa ctcgggcaaa tcccggatgt
    14281 ttcgctaaaa cagctcaacg tgcgtgacca acaggcactg attagcacat tgaccgactg
    14341 gcgcgtacaa cccaacggca ctgaaggcta tcgcactgcc gaagtgacgc tcggcggcgt
    14401 ggacaccaac gaactctctt cacggacgat ggaagcgcgc aaagtgcctg ggctgtactt
    14461 tatcggcgaa gtgatggacg ttaccggctg gctggggggc tataacttcc agtgggcgtg
    14521 gtcgagtgcg tgggcttgtg cgcaggattt gattgcagcg aagtcgtgct gagctatttt
    14581 tcctaaagat gaaaattatt agagtcgcat taaaaatgag ctaattttga tagtggctat
    14641 cttgtgatta ttttctaatg agcccgtgaa ctgaaccccc catgcttaat ataaggtgga
    14701 gggaaaggtg attgaaaact cacccagtgg gctgatcttt ataaaatatg aacaggttga
    14761 aaaaaaaaca gtaactttgc tgttttttat acttaaccac tatgcattaa tgctgcgtta
    14821 tttcattcta cctaagaaaa accagcgtta cgcaaatggt taacgctggt tttatccggt
    14881 acgttgcaat tattttttag cagaacccgc ttctaatatt gaaacgattg agaacaacgt
    14941 aaagcatacc gctcccaaac cgaccagaac ccgcgaagga ataaaatatc ccatatcggt
    15001 ctgcgccatt tccgcaagaa acgatgctag gaaaaggcaa aacaggcagg tgaagacggg
    15061 aatcatcggt attctgttgg ctaacgaaca ggtacgtctc cagaccagtg ccagtagcca
    15121 gacttttgag aatatgctgt aacagatcat tcccaagcaa atgaggataa taccgggagc
    15181 cagtcgggcg cttgcatcag aactgactaa gacgtatatg ccctgcagca ccgtgattga
    15241 gccgagaaaa ataacccaat aacaccacag ccagtgctct ttcgttgaaa acgtattacg
    15301 tgtttgatgg acgattgtgg caacaaggcc aattagacag gcacagattg cggttaaccc
    15361 gagcaatacg tgaccggcga cataatgcgg agttatgtcg gcactacgta acagcgtaat
    15421 agaccaaatg aaaccgagca gggtgagtaa cacgggaacg gcaatgaggc aattaccgat
    15481 taatgaagaa taagcctgta ccggtgttcc gtcgctcttg ctccccgctg cgtttttagg
    15541 aatgagcaga aagtgaccgg atgacgctgc caccgtcgat acacaggcgg caatcatacc
    15601 aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc atcacatcat tacctgctaa
    15661 cagtgcccaa ccccatatca tggtgataat tgaccccgca tagccaataa tggggaacaa
    15721 tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa atgcggtagt
    15781 gaataatgcc aggcatatgg ctgccagaga aatgagcaca tgacccgcga cgaaatactc
    15841 gctttgccct gaactttcca gcacaaagcc acctaacgcg atacaaatca agcccataat
    15901 taaagggatt aatttgaaaa gccacccgat atatatgttc actttaaact cctcaggtat
    15961 ccctgctaat attgaaatgt gcattaatga taatttcagc ctgttaatac aatgacgaga
    16021 tatcattaaa gacaaatgca tagcataact atagcggggt ttactttgca gagcgctact
    16081 tttcagcgtt caaaaggcca tgataaataa atatttatcc taagattaat tcttattctg
    16141 ttgtttttta caattatctt ccagggaggg attctttttc attatttggt aaaagaccgg
    16201 atggtctcgg tgcttaaaat cagcg
//
LOCUS       DAZFDC010000106        15999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFDC010000106 DAZFDC010000000
VERSION     DAZFDC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.106"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(5..1378)
                     /gene="uidB"
                     /locus_tag="ACHJ48_003541"
     CDS             complement(5..1378)
                     /gene="uidB"
                     /locus_tag="ACHJ48_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO4709022.1"
                     /translation="MNQQLSWRTIVGYSLGDIANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(1375..3188)
                     /gene="uidA"
                     /locus_tag="ACHJ48_003542"
                     /pseudo
     CDS             complement(1375..3188)
                     /gene="uidA"
                     /locus_tag="ACHJ48_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            complement(3579..4169)
                     /gene="uidR"
                     /locus_tag="ACHJ48_003543"
     CDS             complement(3579..4169)
                     /gene="uidR"
                     /locus_tag="ACHJ48_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO4709023.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4398..5165)
                     /gene="hdhA"
                     /locus_tag="ACHJ48_003544"
     CDS             complement(4398..5165)
                     /gene="hdhA"
                     /locus_tag="ACHJ48_003544"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO4709024.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5277..6305)
                     /gene="malI"
                     /locus_tag="ACHJ48_003545"
     CDS             complement(5277..6305)
                     /gene="malI"
                     /locus_tag="ACHJ48_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO4709025.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6480..8072
                     /gene="malX"
                     /locus_tag="ACHJ48_003546"
     CDS             6480..8072
                     /gene="malX"
                     /locus_tag="ACHJ48_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO4709026.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVVAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8082..9254
                     /gene="malY"
                     /locus_tag="ACHJ48_003547"
     CDS             8082..9254
                     /gene="malY"
                     /locus_tag="ACHJ48_003547"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO4709027.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9358..10359
                     /gene="add"
                     /locus_tag="ACHJ48_003548"
     CDS             9358..10359
                     /gene="add"
                     /locus_tag="ACHJ48_003548"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO4709028.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(10395..11435)
                     /locus_tag="ACHJ48_003549"
     CDS             complement(10395..11435)
                     /locus_tag="ACHJ48_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO4709029.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11678..11803
                     /gene="blr"
                     /locus_tag="ACHJ48_003550"
     CDS             11678..11803
                     /gene="blr"
                     /locus_tag="ACHJ48_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO4709030.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            12076..12291
                     /gene="ydgT"
                     /locus_tag="ACHJ48_003551"
     CDS             12076..12291
                     /gene="ydgT"
                     /locus_tag="ACHJ48_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO4709031.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            12377..12817
                     /locus_tag="ACHJ48_003552"
     CDS             12377..12817
                     /locus_tag="ACHJ48_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO4709032.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12894..13475
                     /gene="rsxA"
                     /locus_tag="ACHJ48_003553"
     CDS             12894..13475
                     /gene="rsxA"
                     /locus_tag="ACHJ48_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO4709033.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            13475..14053
                     /gene="rsxB"
                     /locus_tag="ACHJ48_003554"
     CDS             13475..14053
                     /gene="rsxB"
                     /locus_tag="ACHJ48_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO4709034.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            14046..>15999
                     /gene="rsxC"
                     /locus_tag="ACHJ48_003555"
     CDS             14046..>15999
                     /gene="rsxC"
                     /locus_tag="ACHJ48_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO4709035.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISMRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARLARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEPVDPR"
BASE COUNT         3818 a         4182 c         3992 g         4007 t
ORIGIN      
        1 aatattaatt agtgatatcg ctgattaatt gctgctgcac ttttttacga ttatcaattt
       61 caaccacgat ttctttgaat tttttatccg tgagcggata aaaccagata ataacgaatg
      121 ccagtagcat aaatccgcaa ggtactaagg caattgatgt gcggatgccc ataattactt
      181 ccggcgtttg cacctgattg gcgatatatc cgcttaatcc caaaataaag gcaggaattg
      241 aaccaccgat tgcctgaccg catttacggg taaatgagaa tagtgaatag gtgagccctt
      301 caattcgcac gccggtcagg tattcaccgt attctacggt atcagcttcc agcgcccaca
      361 tcacggtcat ggtaacgccc tgaccaattg aggcaatggc taacgcaacc aacgccaccg
      421 gcagcgacca gacggaaacc cagaagaaca gcagataacc gcaggttccc agcaaagcgc
      481 caatcaggaa ggtattcttt ttaccgatcc tcgcgaccat ccccggcacc aacggtgccg
      541 atgccacagt accaaccagg ttttgcacca gtaccagcac agtgaacagc ccggtatcat
      601 ttaacacata gcgcacgtag aacaacgacg aggcgctgac cgcgaaggtc gaaatcagca
      661 cacacagcgc accgatgcac aacataaaca gcgggcgatt ccgtttcagg gtttgcagac
      721 tgatcttcaa tgacggctgc gccacgatac gtaccacatt ctcacgcgtc gatttgaagc
      781 agatgaagta aagcaccatt ccggcaatcg ccagcacaat cgtccagaaa tggtataccg
      841 acaccatctc ttccgggctg gagttcttaa tgctcggccc tatcagaaat gccaggcaga
      901 caaaggtcaa tgaagcggca atcccacgag ccgcgcccag acgggcgcgg gattgtggtt
      961 gttgggtcat cgcggtagca agtgaaccat aaggaatatt caccaggctg tagcaaagcc
     1021 cgaggcccat gtaggtcaaa tatgcataca ccactttgct accatggctc cagtcggtcg
     1081 gcacccagaa taccagcacg ctgaagatca ttaacggcgc agtaccgaag agtaaaaacg
     1141 ggcggaattt tccccagcgg gtattcacac tgtccaccac tcgtccggca aagacgtcgg
     1201 cgaaggcatc gaatacccgc accagtaaca gcatggtgcc cgccgcagcg gcaccgacgc
     1261 cagcgacgtc ggtgtagtaa ctcaacagga agagcgcccc cattgcgaag gcgaagttat
     1321 tggcgatgtc accaaggctg tagccgacga tggtgcgcca ggagagttgt tgattcattg
     1381 tttgcctccc tgctgcggtt tttcaccgaa gttcatgcca gtccagcgtt tttgcagcag
     1441 aaaagccgcc gacttcggtt tgcggtcgcg ggtgaagatc cccttcttgt taccgccaac
     1501 gcgcaatatg ccttgcgagg tcgcaaaatc ggcgaaattc catacctgtt caccgacgac
     1561 ggcgctgacg cgatcaaaga cgcggtgata catatccagc catgcacact gatactcttc
     1621 actccacatg tcggtgtaca ttgagtgcag cccggctaac gtatccacgc cgtattcggt
     1681 gatgataatc ggctgatgca gtttctcctg ccaggccaga agttcttttt ccagtacctt
     1741 ctctgccgtt tccaaatcgc cgctttggac ataccaaccg taataacggt tcaggcacag
     1801 cacatcaaag agatcgctga tggtatcggt gtgagcgtcg cagaacatta cattgacgca
     1861 ggtgatcgga cgcgtcggat cgagtttacg cgttgcttcc gccagtggcg cgaaatattc
     1921 ccgtgcacct tgcggacggg tatccggttc gttggcaata ctccacatca ccacgcttgg
     1981 gtggtttttg tcacgcgcta tcagctcttt aatcgcctgt aagtgcgcct gctgagtttc
     2041 cccgttgact gcctcttcgc tgtacagttc tttcggcttg ttgcccgctt cgaaaccaat
     2101 gcctaaagag aggttaaagc cgacagctgc agtttcatca atcaccacga tgccatgttc
     2161 atctgcccag tcgagcatct cttcagcgta agggtaatgc gaggtacggt aggagttggc
     2221 cccaatccag tccattaatg cgtgatcgtg caccatcagc acgttatcga atcctttgcc
     2281 gcgcaaatcc gcatcttcat gacggccaaa gccagtaaag tagaacggtt tgtggttgat
     2341 caggaactgt tcgcccttca ctgccactga ccggatgccg acgcgcagcg ggtagatatc
     2401 acactctgtc cggcttttgg ctgtgacgta cagttcatag agataacctt cacccggttg
     2461 ccagaggtgc ggattcacca cttgcaaagt cccgctggtg ccccttgtcc agttgcaacc
     2521 acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac cacctgccag
     2581 tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc
     2641 caggtgttcg gcgtggtgta gagcattacg ctgcgatgga tcccggcgta gttaaagaaa
     2701 tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat tcccggcggg
     2761 atagtctgcc agttcagttc gttgttcaca caaactgtga tacgtacact tttcccggca
     2821 ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg ctccatcact
     2881 tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg cagcacgata
     2941 cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac gttgcccaca
     3001 taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac agcaattgcc
     3061 cggctttctt gtaacgcgct ttcccaccaa cgctgctcaa ttccacagtt ttcgcgatcc
     3121 agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt ttctacagga
     3181 cgtaacataa gggactcctc attaagataa aaatactggt caacctttaa tctgattagt
     3241 taaccaattc tgtgttgcac acagttatct gtgagaaacc catggaagag aaatggaaat
     3301 aaaaagaact gacaccgcga tcaaaaaacc aaaaactgca atacccacag cgtgatatag
     3361 atcgcattaa tctttaaaac ggcgttgaca gaagccataa aataagcaac cgaacgttcg
     3421 ttcgagcatg ttgggaacga taaactctca tgacatatgg ctacggtgaa tattttaggg
     3481 aggaattccc cgctccggcg agattgtatt ttgtacaggg aaattaaagt atgaaacgcg
     3541 ggctcagttg tcaccagccc gcggaaagag agagagaatc aggatgcggt taagataccg
     3601 ccaatcatcg cgcgtaatcc ctgagcaaac gacgcttcac gcgccaggtc ttccgcttcg
     3661 atatcggcca gtacaccgta ggtcagatcc agcagtaaac gtgaagtcat tgccgtgttg
     3721 atgtctgggc ttatctcgcc tttttgctgg gcatcacgca tccgctgagc aacaaattca
     3781 gtgatcgtca tatgcttatt tttaagcatg gcggcgacct gtgggttacg catcccttcc
     3841 gccataattt cgaccaccag cgcccgttgc ccaaaggctt catgggtgag agagacaata
     3901 gactcgacca tatagtcaac aaaatgaatc ccttcaatcg gttcccggaa acgggccagc
     3961 gccctctcct ggtcctgtaa gataatggcc tgaatcaagg cttctttgga gatgaaatgg
     4021 tgatagagcg tcccgggact aatggcgcag gatttacaga tggctttcat cgaggcactg
     4081 tgaaatccat tttctgaaaa aatctctctg gcagcattga ggatccgggt ccgtgtcggt
     4141 tgtgcttcag tctgcatgtt atccatcatt aaaccattat agttagcgtg aataactagt
     4201 ttaccgcgtt gtcaggtgag cgcctatcat tgctgttgaa tagtgcgaag gcacactcta
     4261 ttcgtatgct tttcatccag ctattggtta accaatggcc gtgatttggc tgccagttgt
     4321 ttagcagaga cgttgatgaa ttgcacacgc tatcccggta aacaacacat acggctgttt
     4381 accggttcgt tagtgtatta attgagctcc tgtaccccac caccggagac ggtgagaatt
     4441 tgtccgctta cccagctggc tgcaggcgag caaaggaaca gcgctgcgtt agcaatatct
     4501 tgcggttggc ccagacgtct gatcggcgtg tgctgtaaca ttttttgttc aatttctggt
     4561 gtaataacgg atttcagggc atcggttaat attgcccccg gcgcaatgcc attcacccga
     4621 atattttttt cacccaggtc aaatgccata tttctgacca gatgactggc cgcagcttta
     4681 gatgatgcat aggaagtcat gtttatattt ttattttctg ccgccataga agtgatggtc
     4741 agaataacgc caccgccatt tttttccatt tctggcgcaa caagttgtga cagatggaaa
     4801 aaagaaaaca cattcagttc ataagcacgg cgaaaatccg ccattggcat atcaaacggt
     4861 ttaggtccac cgccaccggc gttgttaacc agaatatcaa ctttacccag cttactgata
     4921 gcaaagtctg ccagtgcaga gagttcctgt tcggaagtaa tatcacaacg gcaggcaaat
     4981 gcctgaccac ccagttgttg aatttcgtct acaacatggt tagctgcgtc ggcgttaata
     5041 tcactgacca ccacagatgc gccagctgtc gcgaatgtaa tggcgatttc tttaccaata
     5101 cctgcacccg cacctgtgat gatggcgcat tttccgtcga gtctcaggtt gtcagaatta
     5161 aacacataaa cctcctgtga acttcagtaa aaataaaagg caccgcctgt gtgccacatt
     5221 ttttatcatt aaacttgagg aaaggacact accaggtata gcagataaag agaggattat
     5281 ttcgccgcaa tgagccgggc gggaataata agattgcgtg aatgcgtctc ttcatgggtg
     5341 attttttgca tcatgcgatc cgcaagtgtg gtaccaagtt cccgcgcagg cgtgctggcc
     5401 caggtaacgg gtatatcatc aagtgtggtt ggtgtcgcat cggtaaatgc cgccagcgaa
     5461 acctgttgct caaagtaacg atcgactccg ctttccccgc tttgccgccc tgctttcagc
     5521 aaaccaaacc atgcccccat cgcaatagtt tcgttatagc aaaccacggc actgatggtc
     5581 gggttatgac gtaaaagcgc cgtgatagct tccgcggctt gcttctggct ggaagtgcac
     5641 tccaacaccc aatcgctgtg aaacggcagg ccaaatttta gtagagttgc acaatagccc
     5701 cccacccgtt ctgcacgggt taatgaggaa ctttgccctc ccagccaggc gatccgctga
     5761 tgcccattgc gaatgagatg ctccgtcaac aactgtgcag cctgcatgtt gtccgggcga
     5821 accgtatcaa catcatcaag ataactggca cgggaagcga aaatcaccgg gatagctttt
     5881 tcttctgcca tccgtcgcag gtcatcgctg cttcctgcag ccccggcaat taccacacca
     5941 tcgacaccct gattcagtaa cagtgaaaac cgctgtgcca gctgctcgcc gtctttaccg
     6001 ccgtgaagca aaaaaaccat ccgtccctgc gcttccagag cttccgtcaa tccggccgtc
     6061 aattcggcgt aaaacggcgc agataaatca cggacgatca aaccaatgac gccgctttgc
     6121 ccaccgcgca gcgccgacgc ctggcgattg cgcacaaatc ccagctcttc aatggcggcg
     6181 ttaacgcgtt ctcctgtggc ggtagagatt cgccctttgc cactaagcac cagcgaaacg
     6241 gtacttaccg acacgcccgc agccagcgca acatcatgaa tggttatttt tttggcggta
     6301 gccatgcaga tgacctactc cctgattttg tgacagataa aacgttttac cttttattta
     6361 tcttataccc gctattatcg ttgcgtaatg tgatttatgc ctcactaaaa tttgataaaa
     6421 cgttttatct tctcgcgcaa tttactgaat ccagattgtt ctctacgagg agtcgtttta
     6481 tgacggcgaa aacagcaccg aaagtcacgc tgtgggagtt cttccagcag ttaggcaaaa
     6541 ccttcatgtt acccgtggca ttattgtcgt tctgcggcat tatgctcggc attggtagtt
     6601 cccttagtag ccacgatgtc atcaccctgc tcccggtcct gggcaacccc gtgttgcagg
     6661 ctatctttac ctggatgagt aagattggct cgtttgcttt tagtttcctg ccagtcatgt
     6721 tctgtatcgc catcccgctg ggtctggcac gagaaaacaa aggcgtagcg gcattcgctg
     6781 gcttcgtcgg ttatgcggta atgaacctcg cggtaaactt ctggttgacc aataaaggca
     6841 ttctgccaac cacggatgcc gccgttctga aagccaataa catccagagc attcttggga
     6901 tccagtcgat cgacaccggg atcctcggtg cggtgatcgc cggtattatc gtctggatgc
     6961 tgcatgagcg attccataat atccgcctgc cggatgcgct ggcattcttc ggcggtacgc
     7021 gcttcgtacc aatcatctcc tcgctggtga tgggccttgt cggcctggtg attccattag
     7081 tctggccgat tttcgccatg ggtattagcg gcttaggcca tatgatcaat agcgcgggtg
     7141 atttcggacc gatgctgttt ggtaccggtg aacgtctgct gttgccgttt ggtctgcatc
     7201 acattctggt ggcattaatt cgctttaccg acgcaggtgg cacgcaggaa gtctgcggtc
     7261 aaaccgtcag cggcgcattg accatcttcc aggcgcaatt gagttgcccg accactcacg
     7321 gtttttctga aagcgccacg cgtttccttt cgcaaggcaa aatgcctgcg tttctcggcg
     7381 gtctgccagg tgcagcgtta gcgatgtatc actgcgcgcg cccggaaaat cgccataaaa
     7441 ttaaagggct gctgatttct ggcctgatcg cctgtgtcgt tggcggcacg accgaaccgc
     7501 tggaattcct gttcctgttc gtagcgccag ttctgtatgt catccacgcg ctgttaaccg
     7561 gcctcggctt caccgtcatg tctgtgctcg gcgtcaccat cggtaatacc gacggcaata
     7621 tcatcgactt cgtggtgttc ggtattttgc atggtctgtc aaccaagtgg tacatggtgc
     7681 cagtggtggt ggcaatctgg tttgtcgttt actacgtcat cttccgtttc gctatcaccc
     7741 gcttcaatct gaaaaccccg gggcgcgata gcgaagtggc cagttcaatc gaaaaagccg
     7801 ttgccggtgc gccgggtaaa tcaggttaca acgttccggc aatcctcgaa gccttaggcg
     7861 gtgccgacaa tattgttagc ctcgataact gcattacccg tctgcgtttg tctgtgaaag
     7921 atatgtcgct tgttaatgtg caggcactga aggacaatcg ggcaattggc gtagtacaac
     7981 ttaatcaaca taacctgcag gttgttatcg ggccacaagt tcagtcagta aaagatgaaa
     8041 tggccggtct gatgcatact gtccaggcat aaggataaga tatgttcgat ttttcaaagg
     8101 tcgtggatcg tcatggcaca tggtgtacgc agtgggatta tgtcgctgac cgtttcggca
     8161 ctgctgacct gttacctttc acgatttcag acatggattt tgccactgcc ccctgcatta
     8221 tcgaggcgct gaatcagcgc ctgatgcacg gcgtgtttgg ctacagccgc tggaagaacg
     8281 atgagtttct cgcggctatt gcccactggt tttccaccca gcattacacc gccatcgatc
     8341 ctcagtcggt ggtgtatggc ccttctgtta tctatatggt ctcagaactg attcgtcagt
     8401 ggtctgaaac aggtgaaggc gtggtgatcc acacacccgc ctatgacgca ttttacaagg
     8461 ccattgaagg taatcagcgc acagtaatgc ccgttgcttt agagaagcag gctgacggct
     8521 ggttttgcga tatgggcaag ctggaagccg tgttggcgaa accagaatgt aaaattatgc
     8581 tcctgtgtag cccacagaat cctaccggaa aagtgtggac gtgcgatgag ctggagatca
     8641 tggctgacct gtgcgagcgt catggtgtgc gggttatttc cgatgaaatc catatggata
     8701 tggtttgggg cgagcagccg catattccct ggagtaatgt ggcgcgggga gactgggcgt
     8761 tgctgacgtc gggatcgaaa agtttcaata ttcccgcgct gaccggtgct tacgggatta
     8821 tagaaaatag cagcagccgt gatgcctatt tatcggcact gaaaggacgt gatgggcttt
     8881 cttccccttc ggtactggcg ttaactgccc atatcgccgc ctatcagcaa ggtgcaccgt
     8941 ggctggatgc cttacgcgtc tatctgaaag ataacctgac gtatatcgca gataaaatga
     9001 acgccgcgtt tcctgaactc aactggcaga tcccgcaatc cacttatctg gcctggcttg
     9061 atttacgtcc gttgaatatt gacgacaacg cgttgcaaaa agcacttatc gaacaagaaa
     9121 aagtcgcgat catgccgggg tatacctacg gtgaagaagg tcgtggtttt gtccgactca
     9181 atgccggctg cccacgttcg aaactggaaa aaggtgtggc tggattaatt aacgccatcc
     9241 gcgctgttcg ctaaccccaa ttgcgcaacg taaaaaatcg ttgcgcaatc gtggattttt
     9301 accctgcttt gtttttataa tggtgcgcac ttttatatcc agaaaaagag tgcgaccatg
     9361 attgatacca ccctgccatt aactgatatc catcgccacc ttgatggcaa cattcgtccc
     9421 cagaccattc ttgaacttgg ccgccagtat aatatctcgc ttcctgcaca atccctggaa
     9481 acactgattc cccacgttca ggtcattgcc aacgaacccg atctggtgag ctttctgacc
     9541 aaacttgact ggggcgttaa agttctcgcc tctcttgatg cttgtcgccg cgtggcattt
     9601 gaaaacattg aagatgcagc ccgtaacggc ctgcactatg tcgagctgcg tttttcacca
     9661 ggctacatgg caatggcaca tcagctgcct gttgcaggtg ttgttgaagc ggtgatcgat
     9721 ggcgtacgtg aaggttgccg cacctttggt gtgcaggcga agcttatcgg cattatgagc
     9781 cgaaccttcg gcgaagccgc ctgtcagcaa gagctggagg cctttttagc ccaccgtgac
     9841 cagattaccg cacttgattt agccggtgat gaacttggtt tcccgggaag tctgtttctt
     9901 tctcacttca accgcgcgcg tgatgcgggc tggcatatta ccgtccatgc aggcgaagct
     9961 gccgggccgg aaagcatctg gcaggcgatt cgtgaactgg gggcagagcg cattggacat
    10021 ggcgtaaaag ccattgaaga tcgggcgctg atggattttc tcgccgagca acaaattggt
    10081 attgaatcct gtctgacctc caatattcag accagcaccg tggcagagct ggctgcacat
    10141 ccgctgaaaa cgttccttga gcatggcatt cgtgccagca ttaacactga cgatcccggc
    10201 gtacagggag tggatatcat tcacgaatat accgttgccg cgccagctgc tgggttatcc
    10261 cgcgagcaaa tccgccaggc acagattaat ggtctggaaa tggctttcct caacgcagag
    10321 gaaaaacgcg cactgcgaga aaaagtcgca gcgaagtaac aaaaatggat ggtgcaaatt
    10381 gcaccatcca tttttcatgc aaggcacaaa gtcgcgcgat gtttggcgga ttcgatgccc
    10441 agctcaatca actccattac ctggattgcc tggcttgccg gaaccggatt ttcaccatcg
    10501 ccatttaacg catcacgaat cgccgcatag taagccggat aattcccagg caccgtcaac
    10561 agcgtttctt cgacacgttc ctcaccttcc acgcgggtaa gtacgccatc acgcatatcg
    10621 tagccccagt cttcctgcgg cagacgctcg ccatttttca gacgttcttc ctgtggatcg
    10681 aggccatatt tcacataact gcctcgggat ccatgcacga tataccgtgc tgactcagcg
    10741 gctgccagca tggtaccgtg taaaatgact cgccgctgtg gataggacaa gatggcgtgg
    10801 aaataatcgg tcgactgcgc tccgggccgt aactgtgcca aatctaccgt catgctgacc
    10861 ggtaaaccaa atagcgtaat ggcctgatca agaagatgtg gtgctaaatc gtaccagata
    10921 ccgctgcctg ggccgccctg ttcacgccaa cgatcgcgca cctgcggacg gaagcggtca
    10981 aaatgagact caaagtaagc aacttcaccc agcacgcctt ccgcgagcaa accttttagc
    11041 gtcaagaaat cgctatccca acgacggtta tggaatacag acagaacacg ccccaggctt
    11101 tttgccagcg catccagctc tcgcgcttgt gacagtgtca cggtaaaggg tttatcaacg
    11161 accacatgtt tacccgcctc aagcgctgct ttggctaacg ggaaatgggt atcgttgggt
    11221 gtaggaatga caatcaggtc tatgttggga tcgttaaaca gatgcttcgg ctcagagaca
    11281 accgtaaccg ttggccagtc ggcttttact tttgtttcat cactgctgga gattaccgcc
    11341 agttccagcc cgggtgtgcc cgcaatcagg ggcgcatgga aggttttgct cgcataacca
    11401 tacccaatca acccaacacg gatgttgtcg ctcatgtcat tgcctctcat tgaagtatga
    11461 tggctatttg acactatcct ttaccctcgc tcaacagttt aataacctgc cagcaataag
    11521 ggatgttgtt taacttaagt caaaaaaata gcgatttttc caacgacaaa agctaaatat
    11581 cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg ctaagtaaca tttaccccct
    11641 gaagttaatg gatcaatcaa cagagatgtg agttgtaatg aatcgtctta ttgaattaac
    11701 aggttggatc gttcttgtcg tttcagtcat tcttcttggc gtggcgagtc acattgacaa
    11761 ctatcagcca cctgaacaga gtactacggt acaacacaag taagccctgc gcttgtagag
    11821 cgacatgctg cccgtcaggg tgcatgtttt cacttgtcgg atattaaacc aggaatttat
    11881 tatcttgttc ggtgttgttg gtgattgtca gggatagtaa agcgcgttac tcttttcata
    11941 gcagtttttc atccctgaat atttgccatc agcgcattct tattattgta ataacacaag
    12001 cgttcacttt tggtgaaatt ttatcatcac taataatacc tacccctttg gtattaatta
    12061 ttttatggac cttttatgac tgttcaggac tacttattaa aatttcgcaa aatcagttca
    12121 ctcgaaagtc tggaaaaact ctacgaccat cttaattaca ccctgacgga cgatcaggaa
    12181 ctgatcaata tgtatcgtgc tgccgatcac cgtcgcgcag agctggtttc tggcgggcgt
    12241 ttgtttgacc tcggccaggt accgaagtcc gtctggcact atgttcaata aagaaagtag
    12301 cgatcggtta taaaagcctt ataataacgc ccctaaaatt agatttgcct gtttgcgtcg
    12361 ctattctgga gaacctatga ccaccacaac gccacaacgt attggaggct ggttgcttgg
    12421 cccgttggcc tggcttttag tcgctctgtt aagtacgacg ctggcgttgt tgctgtatac
    12481 cgctgcgtta tcttctccgc aaacatttca aacgctcggc gcacaagcac tgacgacaca
    12541 aatcttatgg ggcgtttctt ttattaccgc tatcgccatg tggtattaca cactgtggtt
    12601 gaccatcgct ttctttaaac gtcggcgttg tgttcctaaa cactatatta tctggctgct
    12661 tatttccgta ttgctggcgg taaaagcgtt cgctttttca cccgtggaag atggcatagc
    12721 cgttcgccag ttgctgttta ctttgttggc aaccgcattg atcgtcccct atttcaaacg
    12781 ctcgtcgcgg gtgaaagcga cgtttgtgaa tccgtaataa ccttacagtt aacctgttgt
    12841 cgcctgctct ggattaacgg ataataggcg gcttttttat ttcaggccga aaaatgactg
    12901 actacctgtt actgtttgtc ggaactgtac tggtcaataa ctttgtactg gtcaagtttc
    12961 tcggtctctg tccgtttatg ggggtttcca aaaagctgga aaccgcgatg ggcatggggc
    13021 tggcaacaac gtttgtgatg acgctggcgt ctatttgcgc ctggcttatc gatacgtgga
    13081 ttttgatccc acttaatctg atttacctgc gcaccctggc atttattctg gtgattgctg
    13141 tggtcgtgca gttcaccgag atggtggtgc gcaaaaccag cccggtgctt taccgtttgc
    13201 tggggatttt tttgccgctt atcaccacca actgtgccgt gctcggcgtg gcgttgctga
    13261 atatcaatct cgggcacaat ttcttgcagt cggcgctgta cggtttttcc gccgctgtcg
    13321 gtttttcgct ggtgatggtt ctcttcgcag ccatccgcga acgccttgct gtggctgatg
    13381 tcccagcacc ttttcgcggt aatgccattg cgttaattac cgcaggtctt atgtctctgg
    13441 cctttatggg ctttagtggt ttggtgaagt tgtaatgaat gctatctgga ttgccgttgc
    13501 cgccgtgagc ctgctgggcc tggcgtttgg cgccattctg ggttatgcct cccgccgttt
    13561 tgcggtggaa gacgatccgg tcgttgagaa aattgacgaa atcttaccgc agagccagtg
    13621 tggtcagtgc ggttatcccg gctgtcgccc ctacgcggaa gccatcagct gtaacggtga
    13681 aaaaatcaac cgttgcgccc ctggtggcga agctgtgatg ctaaaaattg ccgagttgct
    13741 taatgtcgag ccgcagccgc tggatggcga agcgcaagag ctaacgcctg cgcggatggt
    13801 ggcggttatt gatgaaaata actgtattgg ctgcactaaa tgtattcagg cgtgtccggt
    13861 agacgccatc gttggcgcta cccgtgccat gcatacggta atgagtgatc tctgtacggg
    13921 ctgcaattta tgtgttgatc cgtgcccgac gcactgcatc tcgttgcaac cggtcgcaga
    13981 aacacctgac tcctggaaat gggatctgaa caccattccc gtgcgtatca ttcccgtgga
    14041 acaccatgct taagttattc tctgcattca gaaaaaataa aatctgggat ttcaacggcg
    14101 gcatccatcc accggagatg aaaacccagt ccaacggtac acccctgcgc caggtaccac
    14161 tggcgcagcg ttttgtcatt ccactgaaac agcatattgg cgctgaaggt gagttgtgcg
    14221 ttagcgtcgg cgataaagta ttgcgcggcc agccgcttac ccgtggtcgc ggcaaaatgc
    14281 tgcctgttca cgcgcccacc tcgggtaccg ttacggctat tgcgccccac tctacggctc
    14341 atccttcagc tttagctgaa ttaagcgtga ttattgatgc cgatggtgaa gactgctgga
    14401 tcccacgaga cggctgggcc gattatcgct ctcgccgtcg cgaagagtta atcgagcgca
    14461 ttcatcagtt tggtgttgcc gggctgggtg gtgcaggatt cccgacaggc gttaaattgc
    14521 agggcggcgg agataagatt gagacgttga ttatcaacgc tgctgagtgc gagccataca
    14581 ttaccgccga tgaccgtttg atgcaggatt gcgcggctca ggtcgtagag ggtattcgca
    14641 ttcttgcgca tattctgcag ccacgcgaaa ttcttatcgg cattgaagat aacaaaccgc
    14701 aggcgatttc catgctgcgc gcggtgctgg cggactctca cgatatttct atgcgggtga
    14761 ttccaaccaa atatccttct ggcggtgcta aacaattaac ctacattctg accgggaagc
    14821 aggttccaca tggcgggcgt tcatccgata tcggcgtatt aatgcaaaac gtcggcactg
    14881 cttatgcagt gaaacgtgcc gttattgatg gcgagccgat taccgagcgt gtcgtaaccc
    14941 tgactggcga agcaatcgct cgcccgggca acgtctgggc acggctgggg acgccagtgc
    15001 gtcatttatt gaatgacgcc ggattctgcc cctctgccga tcaaatggtg attatgggcg
    15061 gcccgctaat gggctttacc ttgccatggc tggatgtccc ggtcgtaaag attaccaact
    15121 gtctgttggc tccctctgcc aatgagcttg gcgaaccaca ggaagaacaa agctgcatcc
    15181 ggtgtagcgc ctgtgctgac gcctgccctg cggatctttt gccgcaacag ttgtactggt
    15241 tcagcaaagg tcagcaacac gataaagcta ccacgcataa cattgccgac tgcattgaat
    15301 gtggggcttg cgcgtgggtt tgcccgagca atattcccct ggtgcaatat ttccgtcagg
    15361 aaaaagctga aattgcggct attcgtcagg aagaaaagcg cgccgcagaa gccaaagcgc
    15421 gtttcgaagc gcgcctggct cgtctggagc gcgaaaaagc ggctcgcctt gaacgacata
    15481 agagcgcagc cgttcaacct gcagccaaag ataaagatgc gattgctgcc gctctggcgc
    15541 gggtgaaaga gaaacaggcc caggctacac agcctattgt gattaaagcg ggcgaacgcc
    15601 cggataacag tgcaattatt gcagcacggg aagcccgtaa agcgcaagcc agagcgaaac
    15661 aggcagaact gcagcaaact aacgacgcag caaccgttac tgatccacgt aaaactgccg
    15721 ttgaagcagc tatcgcccgc gccaaagcgc gcaagctgga acagcaacag gcaaatgcgg
    15781 aaccagaaca acaggtcgat ccgcgcaaag ctgccgtcga agccgctatt gcccgtgcca
    15841 aagcacgcaa gctggaacag caacaggcta atgcggaacc agaacaacag gtcgatccgc
    15901 gcaaagccgc cgtcgaagcc gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    15961 aggctaatgc ggaaccagaa gaaccggtcg atccgcgca
//
LOCUS       DAZFDC010000107        15880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFDC010000107 DAZFDC010000000
VERSION     DAZFDC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.107"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            285..1184
                     /gene="yegS"
                     /locus_tag="ACHJ48_003556"
     CDS             285..1184
                     /gene="yegS"
                     /locus_tag="ACHJ48_003556"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO4709036.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1266..2039)
                     /locus_tag="ACHJ48_003557"
     CDS             complement(1266..2039)
                     /locus_tag="ACHJ48_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO4709037.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(2145..3185)
                     /gene="gatD"
                     /locus_tag="ACHJ48_003558"
     CDS             complement(2145..3185)
                     /gene="gatD"
                     /locus_tag="ACHJ48_003558"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4709038.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3233..4588)
                     /locus_tag="ACHJ48_003559"
     CDS             complement(3233..4588)
                     /locus_tag="ACHJ48_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO4709039.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4592..4876)
                     /gene="gatB"
                     /locus_tag="ACHJ48_003560"
     CDS             complement(4592..4876)
                     /gene="gatB"
                     /locus_tag="ACHJ48_003560"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO4709040.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4907..5359)
                     /gene="gatA"
                     /locus_tag="ACHJ48_003561"
     CDS             complement(4907..5359)
                     /gene="gatA"
                     /locus_tag="ACHJ48_003561"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO4709041.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5369..6631)
                     /gene="gatZ"
                     /locus_tag="ACHJ48_003562"
     CDS             complement(5369..6631)
                     /gene="gatZ"
                     /locus_tag="ACHJ48_003562"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO4709042.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENVDAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6660..7514)
                     /gene="gatY"
                     /locus_tag="ACHJ48_003563"
     CDS             complement(6660..7514)
                     /gene="gatY"
                     /locus_tag="ACHJ48_003563"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO4709043.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKHYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7813..8865)
                     /gene="fbaB"
                     /locus_tag="ACHJ48_003564"
     CDS             complement(7813..8865)
                     /gene="fbaB"
                     /locus_tag="ACHJ48_003564"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO4709044.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9122..10399
                     /locus_tag="ACHJ48_003565"
     CDS             9122..10399
                     /locus_tag="ACHJ48_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO4709045.1"
                     /translation="MKTTTKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10396..11400
                     /locus_tag="ACHJ48_003566"
     CDS             10396..11400
                     /locus_tag="ACHJ48_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO4709046.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFINDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAA
                     "
     gene            11397..12362
                     /locus_tag="ACHJ48_003567"
     CDS             11397..12362
                     /locus_tag="ACHJ48_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO4709047.1"
                     /translation="MSGARLHTLLPELTTRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPEALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFATPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12336..13082)
                     /locus_tag="ACHJ48_003568"
     CDS             complement(12336..13082)
                     /locus_tag="ACHJ48_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO4709048.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13134..13952)
                     /locus_tag="ACHJ48_003569"
     CDS             complement(13134..13952)
                     /locus_tag="ACHJ48_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO4709049.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIHIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPVIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGSVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(14017..14817)
                     /gene="thiD"
                     /locus_tag="ACHJ48_003570"
     CDS             complement(14017..14817)
                     /gene="thiD"
                     /locus_tag="ACHJ48_003570"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO4709050.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRALLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14814..15602)
                     /gene="thiM"
                     /locus_tag="ACHJ48_003571"
     CDS             complement(14814..15602)
                     /gene="thiM"
                     /locus_tag="ACHJ48_003571"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO4709051.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAVNAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15637..15733)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         3905 a         4146 c         3858 g         3971 t
ORIGIN      
        1 gaattacgga catggaggcc cttccgggta actccatgga ggctttaata tattatattg
       61 aagaagccac tgtttagttg aatattaggt atatgctctt ttttgaaatt tatcggtggc
      121 tgccgttagt attcgctgtc cccattgcaa gctcctggtg gtaaccactg aatcctccat
      181 acttgaactg actatttatc ctccgacttt catcctgttc tgactccacc ttttcttttc
      241 tgctctacac tatctataga ccaatcataa aggcacatac gatcatggca gaatttcccg
      301 ccagcttact gattcttaat ggcaaaagta ctgacaatct acccttgcgc gaagcaatta
      361 tgctgttgcg tgaggaagga atgacgatcc atgtgcgggt cacctgggag aaaggcgatg
      421 ccgcacgata tgtagaggag gcccggaagt tgggcgtcgc aacggtgatt gccggtggtg
      481 gcgatggcac cattaatgaa gtttctacgg cgttgattca gtgtgagggg gatgacatac
      541 ccgcgctggg aattttgcca ttaggaaccg ccaatgattt tgccaccagt gtagggattc
      601 ctgaggcact ggataaggcg ctgaaactgg caattgccgg taacgccatt gcgatagata
      661 tggcgcaggt caacaaacaa acctgtttta ttaatatggc gacaggcgga tttgggacgc
      721 gtattaccac agaaacgccg gaaaaattaa aagccgcgct gggtggcgtc tcttacatca
      781 ttcatggctt aatgcgcatg gatactctgc aaccggaccg ttgtgaaatc cgcggtgaaa
      841 actttcactg gcaaggtgac gccctggtca ttggtattgg taacgggcgt caggccggtg
      901 gcggtcaaca attgtgcccg aacgcgttaa ttaacgatgg cttgctgcaa ctgcgcattt
      961 ttaccggcga tgaaattctt ccggctctcg tatcaacctt aaaatctgac gaagataacc
     1021 cgaatattat cgaaggcgct tcgtcgtggt ttgatataca agccccacac gaaatcactt
     1081 ttaatcttga tggcgaaccg ttgagtgggc aaaacttcca tattgaaata cttccggcgg
     1141 cgttgcgttg tcgattacca ccagattgtc cattattgcg ttaatcaaac tattctctct
     1201 tgaaccaggg agaatccgta atgccggatg cggtgtaaac accttatccg gcatacagaa
     1261 caatactaca ccgtaattaa ttttacccgt gtcttctgca atgccagttt atccacatct
     1321 gacacggcat catcggtaat aataatgtca attttttcga ttggcagtac ctgattaaaa
     1381 ccgcgacgat taaatttcga tgaatcgaga acggcaatca ctttatttgc ggcggtgacc
     1441 atcgccccgc taatcgaata accttcatta aaggtcgtaa taccattaac cgcatcaata
     1501 ccatcagcac cgacaaacat taaatcggca ttaatatcct gcaatgaacg ctcggcaatg
     1561 gaaccatgca tcgagcgcgt tttatggcgg accgtgccac cacagacgac cagagtaatg
     1621 tctttatttt cggaaagggc aaacgctgcc gggagactgt tggtaatcac cgtgatattt
     1681 ttggcggtca ttaatccttc ggcgataagc attgtcgtac ttccgctgtc gagaataacg
     1741 gtcatccctt cgtggatcat tttgactgcc gcctgcgcta ttctgttttt tggcgcgctg
     1801 gcaagctgaa atcgctcttt aaacccgact tcctgggtct cggtttcact attaccagac
     1861 attattttcg ccgcaccgcc atgaaagcgc gtaaccacgc ctttttgttc gagaaagcgc
     1921 aaatcggcac ggattgtcgc ttccgaggca gcaaatactc ccgccagatc ctgaacaagc
     1981 acggttccct gttcattcac taattggatg atcttattcc ttcgctcgaa tgaattcatc
     2041 gtcatttacc catattcaat tgtggctagt gtaaacgaag ggggaccatt ggtgaatgat
     2101 ctgttttgca caataccggg ccagcatcac gctggcccgc ggtttcaggg aatgagcaac
     2161 actttgcccg gcatagcatt acgagcgatg tcacgcaccg cctgggcgaa gctttcaaag
     2221 cttccacggt gagcgattaa tggctccagg cttaacttac gttctgtcag caaccggctc
     2281 gccgtttccc actcctgccc cggccaaggg ctggagtagt tcatccaact gccgataacc
     2341 gtcagctctt tacgcaatat tttgccaaac gttgccgatg ttaaatgcag atcctgatgc
     2401 aacgtgccca ccagtgccag ttgggcatga ggaccggcaa tctctaccgc cagttcgaca
     2461 gtttgcggta cgccagccgt ctcgaggata agctgattaa agcgcagttc gcgtaaaacg
     2521 ctctgcattt gcggcgcgct catttcgcta ctgttaaatg tttgcatcgc accgaaagat
     2581 tttgccagtg ccagtttttc tgagctaatg tcgatagccg tcacactctt tgctcccagc
     2641 gcgacagcgc actgaatagc cagcaggcca atggttccgg caccaataat aataacgttt
     2701 ttattctcac aaccttgcgc taaatgaaaa gcatgcaggc caacggtaat cggctcaata
     2761 aaagccccat cctcaatagg catatccgtg ggtagagcaa agacattttt tcgcttaacg
     2821 acaatatatt cggcaaatcc accatcacgc cgtgagccaa taaaatcata ttttgcgcac
     2881 tgggaataaa accctttcaa acactctgga caagtaaaac agggtaataa cggcacacag
     2941 gcaaccgcat cgccagggtg taaatcatca acaccggatc ctaccgcatc aatatagccg
     3001 ctaaattcat ggcctaacgt tattggataa taatgtgcac catttttaaa tatcctgggt
     3061 aaatcggaac cacataagcc cgagctggca atttttaccc gcacctcatc ctgatgttta
     3121 atttcaggaa tgacgctttc tgcaacgcgc acgataccat cagtatcatt caccactgat
     3181 ttcataaaaa ctcctaattg ttaaggggga taacctcccc ctcaggtaaa aattattctg
     3241 cgagaacgac tttctcttgt ttaataaagc cacgcgctct acgccaggtc atgaaaatac
     3301 cggtaagata aattgcgccg ataataatga aaccgggaat attttgcggg gagaaaacct
     3361 gaatcagtaa ccaggtaatg ggagaaccgc cctgatccat tgaagccacc atacccccgg
     3421 cttttaacgc cccagcatta gccgccagtt gggtgtgcaa accaatagtt tgcgtcgcga
     3481 tccacagggt gatgctcata atgatgacac ccgagattaa ggtgcggaac agatttccac
     3541 gatgcaccgc gaccgccatc gccacgaaga agccgatagt ggcaagatcg ccaaacggca
     3601 gtacctgatt accaggaaca caaacagcaa ttaaaatggt gagcggaata aaaatcaggc
     3661 ttgccgatac caccgccgta tgccccagca gtaatgcggg atccaggcca atcaggaact
     3721 cctgaccgcc gaacttcgcc tgtaaacgac tacgggcctg cttagcgatg ggcgttaaac
     3781 catccatgat gggtttaatc acccgtggca ttagcagcat caccgccgcc gtttttaccg
     3841 ccagctgcaa tacacctttg acatcgtaac ccgcgaggat gccgataatc agccccatca
     3901 caaaacccac ggtgacaggc tcgccaaacg ggccaaattt gcgctgaata tcgtcggcgc
     3961 taaatttaat acggttaacg cctgggattt tctcgatgat agcatcgacc agcaccgcaa
     4021 tcggccccat atacgccgac gtaccgtgcg gaatagcaat gccttccagc tcaaagaaat
     4081 ttcgggtatc gcgggcaaac cagtcgccga gcttataaac aaacgccgcg tgaattacca
     4141 cacctgccat ccctatcatc catgaaccgg ttgccagatg cagcaacgcg ccggtgaagg
     4201 tcatatgcca gatattccag atatcaacat ttaccacccg cgtcatacgg gtcagtaaca
     4261 tcgccacgtt aaccagaatc gcaatcggaa tcgccaccag cgcaatttgc gacgcccagg
     4321 tcattggtga agagcccggc cagccgacat cgaccacatg cagattcagg tcgaaatttt
     4381 ccgccatcgc tttcgccgcc gggccaatgg aatccagcat taagccaatc acaaggccaa
     4441 tgccaacaaa gccaatcccg atatgcagac ccgctttaaa gcaatcgcct gccttcatgc
     4501 ctaaaatttt agaaaaaata atgatgacaa tcggcaacat caccgtaggg ccgagatcga
     4561 gaatataacg catgacttct gaaaacatag gtcacccctg taagatagtc agaattttat
     4621 tttgtaatgc ttcgataccg acaccagaaa caaaaggcat gccgtgaact aacggaatat
     4681 cgccaaaact acgatccacc ctggcagtgg tgcatatcaa atgcacgcca tccatatagg
     4741 tttctatttc attaacccga cactggatta attcaacagg aatattatga ctctgacaca
     4801 actctttaat ttcttccgcc gccatcgtag aggtcgcaac cgcgcctccg caagcgacaa
     4861 taatcttgcg tttcataaat agtccttttt attgttacag agggatttat tcgtctgaat
     4921 ctaaaacata ctttgtgaag tattccttta actgagtttc aggaagagtg attagtgtct
     4981 caacgatatc gggctgttgt aacttgccaa ataaacagcg taaaagtttc aattgctgct
     5041 gcggattttc cacaatcaac gcaataacca acgataccgc cacgtcatta tcatcatccg
     5101 cttgctgaaa atgaacttta tttgttggcc ttaacagata aatggctgac gacttagcat
     5161 gaatcgcctc acaatgcggt attgcaatgg cgtgctgctc aagcattatc ccggtaggga
     5221 attctgcttc tctggcaatt aatgcctgtg gccaggtatc atgaaccaca cctttggcga
     5281 gcatctcatt accgatatgg gttaaaactt cgctacgatc gacaaaagaa attccgctac
     5341 gaacaaacag gttagtcata taccgtcctt attccgcaca gccgtagcga taggcgcgca
     5401 aaacatcata aattttatcc ataatcagct gatgcggtat tgctgataat tccccggact
     5461 gaatgcgttc aaattgtttg ggaagatact gactaatcat gcccagtggg atgtccacgc
     5521 cttgcagatt caccatcatc gtttcaacgc tatttttaat ccgactatgc ggccagtaat
     5581 aacgaatacg atccgacagg ctgtaacgaa tatccagtaa tgaatcgtta aaacccgtgc
     5641 gataatattt tttccagtat tgcggttcgt cgagcatcac ttcttcaatt accgccaggc
     5701 aaccgctgcg attttcgggg gcgataagtt cctgctcaat ttgcgccagc gcaaatatcg
     5761 cctcacgtaa agcaaaggtt aatgcgggac cgactttcaa tattgcaaag tgatcgcgga
     5821 ctaattccca ataagccgtc cgggtctggt aatcggtaga atgtgcttca taaaccattc
     5881 gggtattttc tatccattgc gccagcgcct gcgcttcctg cggctgataa tggataatat
     5941 tgctgtgatc aaattccaca cccggctgca ccacgatggc aatcacgcgt gttaatgcct
     6001 ctgtcagccc acgggcaata aaggcctttt gatgcgtacg taaagtattg gcggcatctt
     6061 caacatgggt gatgtgtact gactgaatgg cgctggcctc accgcccgga accggaactt
     6121 cggtgccaat gacatagctc agttgctcac gctggcaatc tgtcgccaca ctttccgcag
     6181 caaagcaaag cacagcagct cgttccgcaa ccgtttctgg tgctaacggt atgggatccc
     6241 ccgcgcagga cattgacgca tcaagatgaa ttttactgaa gccagcacga acatatgcct
     6301 ttaccagctc gacggatttt tccatcgccg catccacatt ttcttgctgc cagcagtttg
     6361 gccccagatg gtcaccgccg agaataatac gctcgcgtgc aaatccgact ttatcggcaa
     6421 tcgcaaaaac aaattcgcga aagtctgccg gtgtcattcc ggtataaccg ccaaattgat
     6481 tgacctggtt tgacgttgct tcaatcagca ctttgcgcgt gctgttgcga tcaaatgcca
     6541 gcgccgcttc gataaccaac ggatgggcag aacagactga acatatgccg atatgttcac
     6601 cagctttatg ccgggcaatt aacgttttca tgttttttcc ttgttaaatg gcaggtgcgt
     6661 tatgccctgc cctcgcagcc acaatcggca atcactttgc tcaccacatc gcgcattgcg
     6721 gatttagccg actgcaaata atcccgggga tcggtcgctt cggggtgctc ggtcaggtaa
     6781 ttttttaacg cctgcgagaa ggcatttttc agctccgttg caacgttgat tttgcatatc
     6841 cccagtttga tggtttgctg aatatcctta gtcgataacc ctgacgcgcc atgcagcacc
     6901 agcggtaagt tcacccactg gcgaatgttc tccagtcgag aaaaatcaag cgccggtgcg
     6961 ctggcataca taccatgagc cgtgccgatg gcgaccgcca gggaatcaat tccggttgcc
     7021 tcggcaaatt cacgcgcctg agcggggttg gtataaaaag catcggcttc attgacttgc
     7081 acatcatctt cctggccgcc aagttgcccc agctccgctt cgacgctgac atcaaagcga
     7141 tggcaaaaat ccaccacctc tttgacccgt gaaatatttt gcgcaaaagg caaatgcgag
     7201 gcgtcaatca tgactgagcg cacgccagaa cgaaccttct gagcgatatc gtcaaatttc
     7261 gtgtgatggt cgagatgaat cgccagtgga tggtgataat gcttcgccat cgcgctgacc
     7321 agcgccaaca gattttctgt accagcatga gtaaatgtgc caggcgttcc ggcgatgatg
     7381 accggcgcat gcaggttggc agcggtttct accaccactt gcatcgtttc gagattgtga
     7441 atattgaatg ccggaaccgc gtaaccgccg cgctgcgcgt tgttcagcat ctgctttgtc
     7501 gataccacgt acattttcac atcctgtcga ttgctttcga tttcaaaaca taatgaaatc
     7561 atttgttttt aaatgtcgag ataacgatca caaaaacgac aatatgaaaa ttaatagagg
     7621 aatgaaaggc aaaaaaaacg gcctcccgga gggaagccgc aacaaagtgc gcgtggtttt
     7681 atgcaggatg cggcgtaaac atatgtggca gcgctgcgta ggcatgataa gacgcttcag
     7741 cgtcgcatca ggcataggtt gccggatgcg gcgtaaacgc cttatccgtc ctacggggta
     7801 gtgccgttcg tatcaggcga tagtaatttt gctatcgaga taaacgtcct gcacggcgtt
     7861 aatcagtttc acgccgtcag ccatcgattt cttgaacgcc ttacgtccaa gaatcagccc
     7921 cattccgcca gcgcgtttgt tgataaccgc agtacgcact gcatcactga ggtcagtttc
     7981 accgcctgca gcaccaccgg agtttatcaa cccggcccgg cccatatagc agttagctaa
     8041 ctgataacgc accagatcaa tcgggttttc gctggttaac ttgctgtaca cgcgatcgtc
     8101 ggtataaccg taattaattg ctttatagcc gccgttattt tccgccattt tttgtttgac
     8161 gatatctgca cctatggttg ccgccagatg gttcgcctga ccagtcaggt cggcggagac
     8221 atggtaatca acgccatctt tcttaaaggc ggagttacgc aaataggccc acagcactgt
     8281 caccatgccc agctcgtgcg cacgttcaaa agccgcagaa atttcttcaa tctggcgacg
     8341 tgactcttcc gagccaaaat agatagtcgc accaaccgcc accgcgccca tgttgaaggc
     8401 ctgctccacg ctggcataca gcgtttgatc gtaggtattc gggtaactta gcgtctcgtt
     8461 gtgattaagt tttacgagaa atggaatgcg atgcgcatag cgccgcgata ccgacgccaa
     8521 cacgccgtaa gttgatgcca cacagttaca gcccgcttcg atcgccagtt caacaatgtt
     8581 tttcgggtca aagtagagcg ggttagcagc aaatgaagct ccggcagagt gctcaacgcc
     8641 ctggtcaacc ggcagaatag aaagatatcc tgtgccagcc agacgcccgg tgttgtacaa
     8701 cgtctgcata ttacgtaaca ccgctggcgg gcgattattg tcaatcatca cgcggtctac
     8761 gtagtcatgt ccggggagat aaagctggtc agaaggaata gtcatacaac ggtgctgtaa
     8821 aaggttgtcg gcgtctttgc caagcaactg cgcaatatct gtcattacta tgctcccgta
     8881 aattccgatt ggatatcggc tatggattgt cctggcccgc cttttgcggg caatcataat
     8941 cctggtcgtt acgggcgagt ttttccatct tttggatgtt ttttcagcgt tttctgttgg
     9001 ctcgattcat cagaaaaaat gttagcgcgg tcaagttttc accgcaaagg tatttaaaag
     9061 gtattattaa gtggtattgt catcgcgtac cttacattac ctgtcatgaa ggaattaaaa
     9121 gatgaaaaca acaacaaagc tgtcgttcat gatgtttgtt gaatggttta tctggggcgc
     9181 gtggtttgtg ccattgtggc tgtggttaag taaaagcggt tttagtgccg gagaaattgg
     9241 ctggtcgtat gcctgcacag ccattgcggc gatcctgtcg ccaattctgg ttggctccat
     9301 cactgaccgc tttttctcgg cgcagaaagt gctggcggta ttgatgttca ctggtgcggc
     9361 gctgatgtat ttcgctgcgc aacagaccac ttttgccggg ttcttcccgt tactgctggc
     9421 ctactcgcta acctatatgc cgaccattgc gctgactaac agcatcgctt ttgccaacgt
     9481 gccggatgtg gagcgtgatt tcccgcgcat tcgtgtgatg ggcactatcg gctggattgc
     9541 ctctggtctg gcatgtggtt tcttgccgca aatgctgggg tatgccgata tctcaccgac
     9601 taacatcccg ctgctgatta ccgccggaag ttctgctctg ctcggtgtgt ttgcgttttt
     9661 cctgcccgac acgccgccaa aaagcaccgg caaaatggac attaaagtca tgctcggcct
     9721 ggatgcgctg atcctgctgc gcgataaaaa cttcctcgtc tttttcttct gttcattcct
     9781 gtttgcgatg ccactggcgt tctattacat ctttgccaac ggttatctga ccgaagttgg
     9841 catgaaaaac gccaccggct ggatgacgct cggccagttc tctgaaatat tctttatgct
     9901 ggcattgccg tttttcacca aacgctttgg tatcaaaaag gtattattgc ttggtctggt
     9961 caccgctgcg atccgctatg gcttctttat ttacggtagt gcggatgaat atttcaccta
    10021 cgcgttactg ttcctcggca ttttgctgca cggcgtaagt tacgattttt actacgttac
    10081 cgcttacatc tatgtcgata aaaaagcccc cgtgcatatg cgtaccgctg cgcagggact
    10141 gatcacgctc tgctgccagg gcttcggcag tttgctcggc tatcgtcttg gcggtgtgat
    10201 gatggaaaag atgttcgctt atcaggaacc ggtaaacgga ctgactttca actggtccgg
    10261 gatgtggact tttggcgcgg tgatgattgc cattatcgcc gtgctgttca tgatcttttt
    10321 ccgcgaatcc gacaacgaaa ttacggctat caaggtcgat gatcgcgata ttgcgttgac
    10381 acaaggggaa gttaaatgaa aacagaacgt attctcggtg ctctttatgg gcaggcgtta
    10441 ggggatgcga tggggatgcc ctccgagctt tggccacgca gccgcgttaa agcacacttt
    10501 ggctggattg accgttttct gcctggacca aaggagaaca acgcggcctg ttattttaac
    10561 cgcgccgaat tcaccgacga tacctcgatg gcgctgtgtc tggcggatgc gttactggaa
    10621 cgtgaaggca agatcgaccc gaatctgatt gggcgtaata ttctcgactg ggcgctgcgt
    10681 ttcgacgcct ttaacaaaaa cgtactaggt ccgacctcga agattgcgct taacgccatt
    10741 cgcgacggta aacccgttgc tgaactggaa aacaacggcg tgaccaacgg cgcagcgatg
    10801 cgcgtctcgc cattaggttg tttgcttccg gcgcgtgatg ttgattcctt tattaatgat
    10861 gtggcgctgg cgtcgagccc gacccataaa tccgatctgg cggttgcggg cgcggtagtc
    10921 atcgcatggg cgatttctcg tgctattgac ggagaaagct ggtcagcgat tgttgattca
    10981 ttgccttcaa ttgcgcgaca tgcacagcaa aaacgcacca ccacgttcag cgcctcactg
    11041 gcagcgcggc tggagattgc gctgaaaatt gtgcgcaatg ccgacggcac tgaatccgcc
    11101 agcgaacagc tttaccaggt cgttggcgca ggtaccagca ctattgagtc cgttccgtgc
    11161 gccattgcgc tggttgaact ggcacaaacc gacccgaatc gctgcgccgt cctgtgcgct
    11221 aaccttggcg gcgatacaga caccatcggt gctatggcga cggcaatctg cggcgcgttg
    11281 catggcatta acgctatcga tcctgcgtta aaggcggagt tggatgcggt aaatcagctt
    11341 gatttcaacc gctatgccac agcgctggcg aaatatcgtc aacaacggga ggcggcatga
    11401 gcggcgctcg attacacacg ctgctgcctg aattaaccac gcgtcagccg gttatggtga
    11461 ttggcgcggc agtcattgat gtgatcgccg acgcttatgc cctcccctgg cgtgggtgcg
    11521 atatcgaact gaaacagcag agcgttaacg ttggcggctg cgcactgaat attgccgtgg
    11581 cgttaaagcg cctcggcatc gaagcgggta acgccttgcc gctcggtcag ggcgtgtggg
    11641 cggagattat tcgcaaccgg atggcaaaag agggcttaat cagcctgatc gataacgccg
    11701 aaggtgataa cggctggtgt ctggcgctgg ttgagccgga tggcgaacgc acttttatgt
    11761 cattcagcgg tgtggaaaat cagtggaatc gccagtggct ggcgcgatta accgttgcgc
    11821 ctggcagcct gctctatttt tccggttatc aactggcctc gccctgcggc gaattattag
    11881 tggaatggct ggaaaagctg caagacgtga cgccgtttat cgattttggc ccacgtattg
    11941 gcgatatccc ggaggcatta ctggcgcgga tcatggcctg tcgaccttta gtgtcgctca
    12001 atcgtcagga ggctgagatt gccgccgaac gttttgcttt atccgcagag ataacaacac
    12061 ttggcaagca atggcaggag aaatttgcca cgccgttgat cattcgtctc gataaagagg
    12121 gcgcatggta tttcagcaac gacgcttctg gctgcattcc ggcatttccc acgcaagttg
    12181 tagacaccat tggggcgggc gacagtcatg caggtggcgt acttgccggg ttggcctctg
    12241 gtctgccact ggcggatgcc gtattactgg gcaatgcagt ggcgtcgtgg gttgtcgggc
    12301 atcggggcgg tgattgtgcg ccaacgcgcg aggaactact cctcgcacac aaaaacgtat
    12361 agatcgctgc gacagtggct aatgctgtac tcaataggcc gttgctgttg gtcaagcgcc
    12421 acttgcttga tcaccagcac cggtattttg ctatctaact gaatatgtga ctgaaactcg
    12481 gcatctggca tccgggcgct aacgcgggaa cgtgtacgtt gtgggtaaat gtgctggctg
    12541 cggaaatagt cataaagaga aattccgatg gcatcaacat catgaattaa atgcgccgga
    12601 acccacgatt cctcaatcga taccgcttct tcatcgacat agcgaatacg cttaagcaaa
    12661 aagacatcac ttcccgcttc gaccgccagc tgctgcgcga cttcttcggg acatttcacg
    12721 acacgcttgt taacccatag cgtatcgggc tttttcccgc gcaataccac ctgctgagaa
    12781 aaacctcgcg cttctttcaa cgagtattcg aagatgttgt tgatctgcgt gccgtaaccg
    12841 cgcgaacgcg tcaccacacc ctcttcttcc agcgcctgca tcgccttgcg caccgtaatg
    12901 cgtgaaacgc cggttaactg acttaaatca cgctcaccgg gcaaaatatt gccatgctcc
    12961 agcaccccgc tgcgcacggc attttttacc gtttcggcaa acttaatata aagcggcgtg
    13021 ttatccgccg ctaaaatacg ctcgttcagt tgggcaatca gctgggtatg cgcttgttcc
    13081 atttgtcttt ttcctgccag ggagtctggt gggagtatat cactgcttct tatttaaggc
    13141 gtttctttaa tcccatcaac gaatgcctgc aactcctcct ctgtgccatt aaacacatta
    13201 aaatccaccg aaccattaat gccatctacc tgcccacggt cggaatgctg ccagaaacgc
    13261 caggccatgc cgtcattgtc cgggcgacgt tgatagtagt gagccaccca ccacggatat
    13321 tcattgaaat agcccgccag attggtgtga taaaaaacgg ctcctgagta aataaccggc
    13381 ttttttcccg tacttttttc gaccattttt agccactgac ttacccgctt gcgtaattct
    13441 tttgccgata attttccccg ttcttctacg tccagcacgg cagggaaatc gccttgtgag
    13501 aaatccaccg tttgcagaaa taatcgcgcc tgaacaggcg ctgctaccga cggggagaaa
    13561 taatgatacg ccccgcgcag caggccattt tcgcggctta gttgccagtt acgcgaaaaa
    13621 taggggtcca ccagcttttc gccttccgtc gccttgataa aggcaaattg taagcggata
    13681 ccgttatcgc gcatttttgc cacccgctgc cagtcgatcc gctcctgcca gcgcgaaaca
    13741 tctattccgt gaatggtgta actggcagga atatgaatag cgaaagattt aaccgggcga
    13801 taaccgtaga agtggatgta gtctgtaatc tgaactgctg tcgaatagaa aaaattcacc
    13861 gtctgacgcg gataaattgc aacgatggaa attagcccaa gagcgcataa tagagcggtg
    13921 agtttttttc gactggtaat tcttagctgc attggtttca tccctgaatg tcagtgccaa
    13981 aggctgacaa taaccaaagc agctatagta cggtgctcac caccaggcgt ggaagtggtg
    14041 aaccggacca ataccgtgac caacttccag cgtgtcggcc tgggctaacg ccgatgaaag
    14101 ccagcttttt gcctcctgta cggtgtcagc ccagtttgta tggcgcgggc gtagtgcagc
    14161 caacgccgca gagagtgtac aaccagtgcc gtgggtgttt ttggtcataa tgcgcggtgc
    14221 ggtaaaccgt tgttcaccct cgcgggtaaa cagccagtcc gggctttgct catcatccag
    14281 atggccacct ttcattagca ctgcgccaca gcccatcgcc aacagcgctc gcccttgttc
    14341 cagcatttcc tgttcggtgc gcgcgtgtgg cgcgtcgagc aaggcagcag cttcgggcaa
    14401 gtttggcgtt attaatgaaa cctgtggcaa taatcgactg cgtagcgtgg caactgccga
    14461 aggtgaaagc agtgggtcgc cactttttgc cagcataacg gtgtcgagta ccacgttttg
    14521 gatctgataa cgttgcaacc gttctgccac cgcttcaaca atatcggttt ccgccaacat
    14581 accgatttta gtggtatcga ttcgcacatc gctgaacacc gaatcgagct gggcggcgac
    14641 aaaatcaggc tcaatgcgat acaccgactg tacgccacgg gtattttgcg ccaccagtgc
    14701 agtaataact gagcaaccat aagcgccaag tgccgagaag gttttaagat cggcctgaat
    14761 ccccgcacca ccactcggat cagtaccggc aatcgtcaga gcgttaattc gtttcatgcc
    14821 tgcacctcct gcgtcaattg ccagagcgca tcaaggaaat gtggaacaaa actgcctggc
    14881 ccctcgcttc tggcgactgc gcgttctccg gcttgtttca tccagtgaca ggcagatgcg
    14941 acattttcca gcatatcgcc tggtaacgca caacaggcag cgacaaccgc cgataatgca
    15001 cagccagttc ctaccacttt ggtcattaac ggatcgccac cgtgaatgcc aacggcgcga
    15061 tggccatcgg taacataatc cacctcgcca gtgaccacga cgattgcgcc agtttcccgt
    15121 gccagtgttt gtgcagcggg tatcgcgtta actgcggcgt cagtggtatc cactccccgt
    15181 ccaccattag caacgccagc taatgccatg atttccgaag cattaccacg tatcgctgcc
    15241 ggtttaaaag ataaaagttc atgacaaaaa tggcggcgat aatcgagcgc accaaccgct
    15301 actggatcaa gcgtccaggg tgtttgagag ctttttgctt gctcaacggc agcacgcatc
    15361 gcctgtgcgc gtggctgcgt cagtgtgcca acgttaatca acaaggcact ggcgatagcc
    15421 gcaaactgac tggcctcttc ggtttcgata accatcgctg gcgatgcacc gagcgccagc
    15481 aaggtattgg cagtaaaggt ttgcaccaca tcgttggtca tgcagtgcac aagaggggaa
    15541 tgttggtgaa aaaggtgtaa cgtgtgcgca gattgcgcgg aactcagcag gtcgacttgc
    15601 atagtttgct cctgccataa cgtgaagaag caatgacctg gtggtccgtg acttccctac
    15661 gctggcatta tccagatcag gtgatacggg tatttctcag ccttcacgca gaagggcacc
    15721 ccgagtcgtt tggttgaatc tctatatttt gtgccagctt aggggaagag gcttgcagat
    15781 gcaagctcgc ctccattcat tacgtcttca ctgtgtaccc catatcagta atgaaaaaga
    15841 aacaattcac acccatcatt caggtggtaa aaactgtaga
//
LOCUS       DAZFDC010000108        15856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFDC010000108 DAZFDC010000000
VERSION     DAZFDC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.108"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1053)
                     /gene="espY3"
                     /locus_tag="ACHJ48_003572"
     CDS             complement(<1..1053)
                     /gene="espY3"
                     /locus_tag="ACHJ48_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspY3"
                     /protein_id="HFO4709052.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDATSDLDSYLKLDFDRLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKDGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDMGNCNCSNVNFEHTYFYGVKFTNA
                     NCTNANFNNCRFKKCDLTNMNCTGAILDNAMIYGKEKEPEMQYPEADQIIQRITYQKS
                     DGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKTEAESTEE"
     gene            complement(1284..2258)
                     /gene="yajO"
                     /locus_tag="ACHJ48_003573"
     CDS             complement(1284..2258)
                     /gene="yajO"
                     /locus_tag="ACHJ48_003573"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO4709053.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFSRREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(2313..4175)
                     /gene="dxs"
                     /locus_tag="ACHJ48_003574"
     CDS             complement(2313..4175)
                     /gene="dxs"
                     /locus_tag="ACHJ48_003574"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO4709054.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(4200..5099)
                     /gene="ispA"
                     /locus_tag="ACHJ48_003575"
     CDS             complement(4200..5099)
                     /gene="ispA"
                     /locus_tag="ACHJ48_003575"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO4709055.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKAQDLIDDARQSLKQ
                     LAEQSIDTSALEALADYIIQRNK"
     gene            complement(5099..5341)
                     /gene="xseB"
                     /locus_tag="ACHJ48_003576"
     CDS             complement(5099..5341)
                     /gene="xseB"
                     /locus_tag="ACHJ48_003576"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO4709056.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5547..6995
                     /gene="thiI"
                     /locus_tag="ACHJ48_003577"
     CDS             5547..6995
                     /gene="thiI"
                     /locus_tag="ACHJ48_003577"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO4709057.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     DGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTG
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFKNVKVYRP"
     gene            complement(7049..7639)
                     /gene="yajL"
                     /locus_tag="ACHJ48_003578"
     CDS             complement(7049..7639)
                     /gene="yajL"
                     /locus_tag="ACHJ48_003578"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO4709058.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLIRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(7602..8513)
                     /gene="panE"
                     /locus_tag="ACHJ48_003579"
     CDS             complement(7602..8513)
                     /gene="panE"
                     /locus_tag="ACHJ48_003579"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO4709059.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAACRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRTLRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8681..9172
                     /gene="yajQ"
                     /locus_tag="ACHJ48_003580"
     CDS             8681..9172
                     /gene="yajQ"
                     /locus_tag="ACHJ48_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO4709060.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(9300..10664)
                     /locus_tag="ACHJ48_003581"
     CDS             complement(9300..10664)
                     /locus_tag="ACHJ48_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4709061.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLRNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(10813..11703)
                     /gene="cyoE"
                     /locus_tag="ACHJ48_003582"
     CDS             complement(10813..11703)
                     /gene="cyoE"
                     /locus_tag="ACHJ48_003582"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO4709062.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11715..12044)
                     /locus_tag="ACHJ48_003583"
     CDS             complement(11715..12044)
                     /locus_tag="ACHJ48_003583"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO4709063.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(12044..12658)
                     /locus_tag="ACHJ48_003584"
     CDS             complement(12044..12658)
                     /locus_tag="ACHJ48_003584"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO4709064.1"
                     /translation="MATDTLTHATVHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(12648..14639)
                     /gene="cyoB"
                     /locus_tag="ACHJ48_003585"
     CDS             complement(12648..14639)
                     /gene="cyoB"
                     /locus_tag="ACHJ48_003585"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO4709065.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(14661..15608)
                     /gene="cyoA"
                     /locus_tag="ACHJ48_003586"
     CDS             complement(14661..15608)
                     /gene="cyoA"
                     /locus_tag="ACHJ48_003586"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO4709066.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGFATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPNLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         3978 a         4092 c         3992 g         3794 t
ORIGIN      
        1 ttcttctgtt gattctgctt ctgtttttaa tatcggatta aaaaggtcgg tacggtaaag
       61 tttcgtgcct ttcatcctga cgatgtcagg aagaatagtg tttgaaagat tagcctcgct
      121 aaagtccaca ttttggcaat cactttctga taaatctgcc caattgaaag tggttttcac
      181 acaggagcag ttagttagaa tcatcccttt cgtttcattc ccatctgatt tctgataagt
      241 tatcctttgt attatttgat ctgcttctgg atattgcatc tcaggttctt tttctttacc
      301 atatatcatc gcattatcaa gaattgcgcc agtgcaattc atatttgtca ggtcgcattt
      361 cttaaatcta caattgttaa aattcgcatt tgtacaattc gcatttgtaa acttgacgcc
      421 ataaaaataa gtatgttcga agttgacatt tgagcagtta cagtttccca tatctatacc
      481 gaaatctata tcgggatctt tattgggatc tttaccggaa atattgctgt gtgctagata
      541 tagaccgctg aaatcgtaat atccattgtc atccttttct attgggagtg aataagtctt
      601 tactacctcg ttaacaatag agatagttcc acgtatcatc agattttctc cgtctttata
      661 aaaaataatg ttatgtttga tggtactgtt gtatggctct ggcaatgagg atgcaatttt
      721 attccagaaa caacaaagcg tggtctgttt ttgtttgggc gagaggcgat caaaatccag
      781 ctttaaatat gagtctaaat ctgacgttgc atctatatta aagttgggat ctggagttaa
      841 cgctgaatta taatttagaa ctgacaatgt attgatgtat ttatgaatcg atttaaatgc
      901 cttttgttta tcactgtcca gaaaaaaatc acgaattttg ttccacagcc tttcaataag
      961 attttgattt aaatcttgct tcattgagtt tcttaaatct gcaaccggta catcagtaaa
     1021 caggatcggt tgtttggtga tggttttcat catgattgta atcctcttac gttgactaaa
     1081 taagacagtt ttaggggata ttacctggag aaactatcaa aaatcgctca aagggatgaa
     1141 gacgatgaat agctgcaaac tcattcacga agaatttctc ttgcagaggt ggattatggt
     1201 tacagaaggt gatgggaggt acgttgtccc catcatcgca gacgatgggg aattcagcag
     1261 ataaaatggg tgaaaagcga aatttactta aatcctacga ccggatgcgg tttatacggt
     1321 gtttccagtt cagcaatctg ttccggcttc aaagtgatat ccaccgcgtt cagcagctca
     1381 tcaagctgtt cttcccgcga agttccgata atcggtgcgg caatgcccgg tttactcaac
     1441 aaccaggcca gcgcaacttg tgctcgtgtt gcaccgagtt cttcactgac gcccgttaac
     1501 cgctctgcga tctgcgcgtc attttcatcg ctttctttat agagattttt ccccacctca
     1561 tcagacacca gtcgtgcggt agtttctccc cacggacgcg tcagtcgccc ccgcgccagc
     1621 ggactccacg gaatgaccgc cacgccttcc tgataacaca gcggcagcat ctcgcgctct
     1681 tcttcgcgat aaatcagatt gtagtgatcc tgcatactga caaactgcgc ccagccgtgc
     1741 tgtttttgta gttccagtgc ctgagcaaac tgcgaagcgt gcatggatga cgcgccgata
     1801 taacgcgctt tcccggcttt taccacgtcg ttgagggctt ccagcgtctc ttcgatcggc
     1861 gtgttgtaat cccagcgatg aatttgcagg atatcgacat aatccatgcc gagacgtcgc
     1921 aggctgtcgt cgatagagcg caaaatttgt gcacgggata atccttccgg taaatcacca
     1981 acgcgatgga acactttggt ggcaacgacc acgtcttcac gacgggagaa atcccgcagt
     2041 gcgcgaccga cgatctcttc gctgctgcca tcggaatagc tattggcggt atcaaagaaa
     2101 tttatgccgc cttccagtgc gcgtttaatg atgggacggc tgctctcttc cggcagtgtc
     2161 catgcgtgat taccacgatc tggctcgcca aaggtcatac agccgaggca aagtcgggaa
     2221 acgcgaaggt cggtttttcc taaggggttg tattgcatgc tgccactcct gccatgaata
     2281 atttcttaag catagcagga gtggagtagg gattatgcca gccaggcctt gattttggct
     2341 tccataccgg cggcatcgag gccgagttcg gcgcgcattt cttcctgagt tccttgcgga
     2401 ataaagaagt ccggcaggcc aatgttcagc acgggtactg gtttacgatg ggccatcagc
     2461 acttcgttca ctccgctgcc tgcaccgccc ataatggcgt tttcttctac ggtaaccagt
     2521 gcttcatgac tggcggccat ttccagaatt aacgtttcat caagcggttt cacaaaacgc
     2581 atatcgacca gcgtggcgtt cagcgattcg gcgactttcg ccgcttctgg catcagcgta
     2641 ccaaagttaa ggatcgccag tttctcgcca cgacgcttca caatgccttt gccaattggt
     2701 agtttttcca gcggcgtcag ttccacgccg accgcgttgc cacgcgggta gcgcaccgct
     2761 gacgggccat cgttatagtg atagccggta tagagcatct ggcgacattc gttttcatcg
     2821 ctcggggtca taatgaccat ttccggtatg cagcgcaggt aagagagatc aaaagcgccc
     2881 tggtgggttt gaccgtcagc accaacaatg cccgcgcggt cgatggcgaa caggaccggg
     2941 agtttttgaa ttgccacgtc atgcagcacc tgatcatagg cgcgttgcag gaaggtggag
     3001 taaattgcca cgatcggttt gtagccacca atcgccagac cggcggcaaa ggtcaccgcg
     3061 tgttgctcgg caatcgctac atcaaaatag cgatccggga atttacgtga aaactcgacc
     3121 atgccggaac cttcacgcat cgccggagta atcgccatca gtttgttatc tttcgctgcc
     3181 gtttcgcaca accagtcgcc aaagattttt gaatagcttg gtaaaccacc gctacttttc
     3241 ggcaaacaac cgctggaggg atcaaattta ggcacggcgt ggaaggtgat tgggtctttt
     3301 tctgccggtt cataaccacg accttttttg gtcatgatat gcaggaactg cgggcctttc
     3361 aggtcgcgca tgttcttcag cgtggtgata agccccagca catcgtgacc gtcaaccggg
     3421 ccgatgtagt taaagcccag ctcttcaaac aacgtgccag gcaccaccat gcctttaata
     3481 tgttcttcgg tacgtttgag cagctcttta attggcggaa cgccagagaa aacttttttc
     3541 ccgccttcgc gcagtgaaga gtaaagctta ccggaaagca gctgcgccag atggttgtta
     3601 agcgcgccaa cattttcgga aatcgacatt tcattgtcgt tgaggaccac cagcatatca
     3661 ggacgaatat cgcccgcgtg attcatcgct tcaaacgcca tgcctgcggt aatcgcgccg
     3721 tcgccaatca cacagacggt gcggcgattt ttgccttctt tctcggcagc aaccgcaata
     3781 ccaattccgg cactgatgga ggttgatgaa tgcccgacgc ttaatacgtc atattcgctt
     3841 tcgccgcgcc acgggaacgg gtgtagaccg cctttctgac ggatggtgcc gattttgtcg
     3901 cggcgtccgg tcaaaatttt atgcggataa gcctgatgcc ccacatccca aatcaattgg
     3961 tcaaacgggg tgttatagac atagtgcagc gccacggtca gttcgaccgt gcccagcccg
     4021 gaggcgaagt gcccgctgga acggctcacg ctgtcgagta aatagcggcg cagttcgtcg
     4081 cagagtttcg gtaaactctc ttttggcaac agtcgtagct cctgggtgga gtcgaccagt
     4141 gccagggtcg ggtatttggc aatatcaaaa ctcatcaggg gcctattaac acttattgtt
     4201 tatttattac gctggatgat gtagtccgct agcgcttcca gtgccgaagt atcgatcgac
     4261 tgttcagcca gttgtttcag cgactggcgg gcatcgtcga tcagatcctg ggctttcttc
     4321 cgggcttgct caagacccag gagtgcaggg taggtacttt tgccaagctg ctggtcggca
     4381 ccctgacgtt ttcccaacgt tgcagtatct cccaccacat ccaggatgtc atcctgaacc
     4441 tggaaggcaa ggccgatgct ctctgcgtac ttgtcgagta ctggtagagc acgacgtcct
     4501 ttatctccgg cgcttaatgc accaaggcga acggcggcgc gaattaatgc gcctgtttta
     4561 tgacgatgaa tacgctcaag cgcgtccaga ggtacgtgtt tgccttccgc gtctaaatcc
     4621 aacgcctgac caccacacat tccggcaata ccgctggcgc tcgccagttc agaaatcatc
     4681 gaaattctgt cgcgatccga cacttctggc atatcagcat cgcttaaaat cgagaacgcc
     4741 agcgtttgta aagcgtcgcc agcgagaatc gcgttcgctt cgccaaactt cacatggcag
     4801 gtcggcaagc cgcgacgcag atcgtcatca tccatcgccg gtaaatcatc atgaattaat
     4861 gagtaagcgt ggatacactc tacggcggca gcgggtgcgt ccagcgtgtt tgtgctaacg
     4921 ccgaacatat gaccggtggc ataaaccagg aaaggtcgca ggcgcttacc acctaataat
     4981 gcgccatact gcatggtttc gaccacggga gtgttctgaa agggcagtgg ggcgataaaa
     5041 cggctcagcg cctggttggc ctgcttaacg caggcttcga gttgctgcgg aaagtccatt
     5101 actcattgtc cggtgtaaaa ggggttagag aggcgtcttc gttgtcagac agcagaattt
     5161 gtacgcgctg ttcggcttgt tgtaatttgg cctgcccctg acgtgccagc tgcacgccgc
     5221 gttcgaactc gttcagcgcc tcttccagcg gcaggtcgcc actttccaga cgggttacaa
     5281 tctgttccag ctcgctcagc gccttttcaa agctggcggg cgcctcattt ttcttcggca
     5341 taatgaatgt ctgactctca atatttttcg ccccgtcatg gtaacggact cagggcaaat
     5401 agcaaataac gcgcaatggt aaggtgatgt gcgcagcaaa gcgatgttag tggtatactt
     5461 ccgcgcctgg atgcagccgc aggtgtgggc tgctgtattt ttccctatac aagtcgctta
     5521 aggcttgcca acgaaccatt gccgccatga agtttatcat taaattgttc ccggaaatca
     5581 ccatcaaaag ccaatctgtg cgcttgcgct ttataaaaat ccttaccggg aacattcgta
     5641 acgttttaaa gcactatgat gagacgctcg ctgtcgtccg ccactgggat aacatcgaag
     5701 ttcgcgcgaa agatgaaaac cagcgtctga ctattcgcga cgccctgacc cgtattcctg
     5761 gtatccacca tattctcgaa gtcgaagacg tgccgtttac cgacatgcac gatattttcg
     5821 agaaagcgtt ggttcagtat cgcgatcagc tggatggcaa aaccttctgc gtacgcgtga
     5881 agcgccgtgg caaacatgat tttagctcga ttgatgtgga acgttacgtc ggcggcggtt
     5941 taaatcagca tattgaatcc gcgcgcgtga agctgaccaa tccggatgtg actgtccatc
     6001 tggaagtgga agacgatcgt ctcctgctga ttaaaggccg ctacgaaggt attggcggtt
     6061 tcccgatcgg cacccaggaa gatgtgctgt cgctcatttc cggtggtttc gactccggtg
     6121 tttccagtta tatgttgatg cgtcgcggct gccgcgtgca ttactgcttc tttaacctcg
     6181 gcggcgcagc gcatgaaatt ggcgtgcgtc aggtggcaca ttatctgtgg aaccgctttg
     6241 gcagctccca ccgcgtgcgt tttgtcgcta ttaatttcga gccggtagtc ggggaaattc
     6301 tcgagaaaat cgacgacggt cagatgggcg ttatcctcaa acgtatgatg gtgcgtgccg
     6361 cgtctaaagt ggctgaacgt tacggcgtac aggcgctggt caccggcgaa gcgctcggcc
     6421 aggtgtccag tcagacgctg accaacctgc gcctgattga taacgtctct gatacgctga
     6481 tcctgcgtcc gctgatctct tacgacaaag agcacatcat caacctggct cgccagattg
     6541 gcaccgaaga ctttgcccgt actatgccgg aatactgcgg tgtgatctcc aaaagcccga
     6601 cggtgaaagc ggttaagtcg aagattgaag cggaagaaga gaaattcgac ttcagcattc
     6661 tcgataaagt ggttgaagaa gcgaataacg ttgatatccg cgaaatcgcc cagcagaccg
     6721 ggcaggaagt ggtggaagtg gaaaccgtca atgacttcgg cccgaacgac gtgattctcg
     6781 atatccgttc tgtcgatgaa caggaagata agccactgaa agtcgaaggg attgatgtgg
     6841 tttcactgcc gttctacaag ttgagcacca aatttggcga tctcgaccag aacagaacct
     6901 ggctactgtg gtgtgagcgc ggggtaatga gccgcctgca ggcgctctat ctacgcgaac
     6961 agggctttaa gaatgtgaag gtgtatcgtc cgtaatttgt ggtttttacg tcgcatctgg
     7021 tcagatgcga cgtttgccgc atccgacact actcgtaata attataaatc cctgccgcca
     7081 tcaccagttg tgatgccact tcatgggctt tctcgcgccc aaccagcagg tcgataattt
     7141 tcagaccaaa gtcgatagct gtacccggcc cctggctggt cagcaatttt acccgtgcat
     7201 cccagacgac gcgcttgtcc tgccattgtt cagcgggaat tttgtctttc agcgtcggga
     7261 agccggtcat attgccaatc ggaaagatat cgtgcggcac cagcacggtg gctggcgcgg
     7321 cgcaaatagc cgcgacgata cgcccggaac ggtggaactg tttaacggtt tcaaccagca
     7381 gggtactatc gcgaaaacac tccgcgcctt taatgccacc aggcagcacg atcacgtcat
     7441 attcgccatc agccacttcg accagcggcg catccgccag cagcttcacg ccgcgcgagc
     7501 aggtaatcgc caggttgcca tcgctggcta cgctggcagt ggtgactttg ataccgccgc
     7561 gaatcaagag gtcgatcgtg gtgacggctt cagtctcttc actaccaggg gcgaggcaaa
     7621 ccagtgccga tgcgctcata ttcactctcc tttcttttta ccatttcaaa caggcgggtg
     7681 ttttccggta cggcaatccc atgcgcgcgg gcgcggcgta agagaaaacc attgatatag
     7741 tcgatttcgg tgtggcgcag cgtgcggata tcctgcaaca tcgacgagat attttccgct
     7801 gtggcatcaa tcacctgcat cacgtaatca cgtaaatctt ccgctgaagt atgatgccct
     7861 tcgcgttcga tcactgccgc gacttcttcg catatttgca taatttcttg cggatgatga
     7921 cgtaattcac cgttcgggca attccagatg gcagtcagtg gattaatcac acagttgact
     7981 gccagcttgc gccacagctc ggcgcgaata ttgttatgcc aggcaacgtc aggcaacacg
     8041 gtttgcaata tatccgccag ataactgtaa tcgccgtcct gttgccgtgc tggaccaata
     8101 tgcgtgatac cgtttgccac atgaataatg acattgccgt cgcggcaggc ggcatgggtg
     8161 gtggtgccca tcagtaatgg ctgctgaatg ttttgcaatt cttcgatggt gcccatgccg
     8221 ttgtgaatta acagtattgg cgtagttaca ggcagtgtgg acgcgaggct tttgacggca
     8281 tcggaaacct gccatgcttt cagcgtcacc aggagcagat cgctggtggc aagaaaatcg
     8341 ggatcgttgg cggtcagcga ttcgttaaat atcgaaccat cagtctcaac cagattcaca
     8401 ctacaataag gttgcggtac gcgcagccag ccctgaactt cgtgaccctg tttacaaagt
     8461 gctgtaagcc ataattgccc taaggcaccg catcccaata cggtaatttt cattgttcct
     8521 cctcacccgc aaccactccg ggtgttcaat aaggctatcc cttaattgtg catgctgttg
     8581 cgactatgca caattaaggg atacgtcctg gtgcaggact gttggttatt taactttgca
     8641 ggtattatgc ttcgcatcaa aaatgaaggg agaggaaaag atgccatctt tcgatattgt
     8701 ctctgaagtt gatcttcagg aagcacgtaa cgcggtcgat aacgcgagcc gcgaagtgga
     8761 gtcccgtttt gacttccgta acgttgaagc ctcatttgag ctgaacgacg ccagcaaaac
     8821 catcaaagtg ttgagcgagt ccgacttcca ggtcaatcag ttgctggata ttctgcgtgc
     8881 caagctgctg aagcgcggca ttgaaggcag ttcactggat gtaccggaaa atatcgttca
     8941 tagcggtaaa acctggtttg tggaagcgaa actgaaacag ggcattgaga gcgccactca
     9001 gaaaaaaatc gtcaagatga tcaaagacag caaactgaaa gtgcaggcgc aaattcaggg
     9061 cgatgagatc cgtgtgacgg gcaaatctcg tgatgattta caggctgtca tggcgatggt
     9121 gcgcggtggc gatctcggtc agccgttcca gttcaaaaac ttccgcgatt aatcgcgaca
     9181 acctgcgctt tgttcatgtc agatgcggca tgaacgcctg gttcggccta caaagttttg
     9241 taaatcagga acttagtagg cctgataagc gtagcgcagc aggcaatttc gcctcgcttt
     9301 tatgcctggc gaattgcctg ttcaacatca aagcgattcg tcactttgct gtcgattttc
     9361 acataagctg aatgttcttc ttctgcaatc aacacttctt tgatgccttc agtttccagc
     9421 aaacgcactt ttaacgcctc gtttgcggca atgttcgccg ggatttcaat gcgcaaactg
     9481 ctgacatacg gcggttcttt catggtactg gcgactgcca gccacactgc ggccagcatt
     9541 gcgccagcga gaaatacccc ctgaccgtca aacatgccgt caatccagcc gcccagcgaa
     9601 ccgccaatcg ccacgccaag aaactggctg gtggagtaaa cacccatcgc cgtacctttg
     9661 taacctgctg gcgactcttt actgataagt gagggcagga gggcttccat caaattaaac
     9721 gccacaaaga aaagctgcac gccgaccacc agttgccaga actgcgtttg cgcgttcctc
     9781 aacacaattt ccgcaaccac gatcaacccg acgcagaaga caaagacttg cttcattttg
     9841 cgcttaactt cagcgtagat aatgaaaggc acgaccgagc caaaggcgat tagcattgtc
     9901 gccagataga ccttccagtg ttcagccgcc gggaaccccg catcagccag ttgtccgggc
     9961 agggcaacaa acgtcgacat cagcaaaata tgcagacaca taatgccaaa gttgagtttc
    10021 agcagccgcg gttccgccag caccttactg aaactgcctt tcaccattcc ggactcacga
    10081 ttaagtacgt gagtgctact gttgggcaca acccaaatgg tcaacgcaat gccggtcgtt
    10141 gccagaatag cgatcatcca gaacagcgcg tgcagcccaa gtttgtgagt gatgatcggg
    10201 ccaagcacca tcgcaatggc aaaggtaatg ccaaagctca cgccgataaa cgccattgct
    10261 ttggtgcggt tttgttcgcg cgtgagatcg gaaagcagcg ccataacggc agcggcaatc
    10321 gcaccggagc cttgtagcgc ccgacccaga ataattcccc agatggagtc agagagcgca
    10381 gcgataacgc taccggcggc aaacactgcc agcccaccga caattaatgg tttgcgacca
    10441 atgcggtcgg aaagcaggcc aaacggaatc tgaaaaacgg cctgagtcag accataaata
    10501 ccaatggcaa taccgattaa tgcttcgctg gcaccttgca gagccatgcc gtacgtggtc
    10561 agaaccggca gaaccatgaa catgcccagc atgcgcaacg agaatacggt ccctaaaccc
    10621 caggtcgcgc gccgctcacc tggcgtcatt ttataatcgt tcattaccac ctctgtttta
    10681 aattcgcgac tagtgtaaag cggcaaacga atggggtaaa catgcggatt ttttagtaaa
    10741 tatttcgaga gggatatgtt tctaatgcta agaaaaaagg tgccgtagca cctttttaat
    10801 agagaggttg tgttaccaca cagcagccag cagcgtatgc gagtccggca ccataaaatc
    10861 aacggacatc atcaccgaga gggcagtgat ggcgatgata gagaagccga acagcttgcg
    10921 cgcccagatt ctgtcatcag caactttata accgcgcaga gccataccta accaccagac
    10981 gctaaccgcc gcggcgacca ccagatattt atatccagcg taaccgccaa gagagagcat
    11041 cagcgtggca acggcaaagg cgatgatata cagcgtgatg tgattcttcg ccaccgaaat
    11101 gccttttacc actggcaata ccggaatgtt tgccgcctgg taatccttaa agcggaaaat
    11161 ggcgatggca taggagtgag gcatctgcca caggctgaag atagccagca ggatcgctgc
    11221 gccgctatcg aactcgccgg ttactgcaca gtagccaatc accggcggcg cagcgccgga
    11281 gagcgaacca atcaatgtgc cgtagacgga gtggcgtttc atgtacaggc tataaacgcc
    11341 gacataaacc acaaagccca tcacccccag ccagcaggcc agcggattcg cgccaaacca
    11401 cagcagcata aagccagcaa tacccagcaa ggtggcgtac accagcgaga cagcaggaga
    11461 gatcaggcct ttaaccagca cccgattctt cgtcctttcc atctttctgt cgatatccct
    11521 gtcgatgtag ttgttaaaca cacaacccga cgccacaacc agtgacaccc caaccagcgt
    11581 gtagataaac aggggataat caatgctgcc ctttgaggcc agcaggaatc ccccaatcac
    11641 cgagatcagg ttgccaaaga tgatgcctgg tttcgttact tgcaggtatt gcttaaacat
    11701 cataaccgcc gctcttagtg catcatcatg ttgtagttga ggttccacat aatccagatg
    11761 gagcctacaa ctaggatagc gatgattagc acggtgaaga caaacgccgt catgttccag
    11821 ccttcatctg atttggtatt catgtgcagg aagcacacca gatgcaccag aacctgtacc
    11881 actgccattg ccaggattgt tcccagaatt acggccggag aggcagcccc tgtcatcacc
    11941 atccagaacg gaatcaccgt cagaatgatc gacaggataa agcctgtcat gtaggttttt
    12001 acgctgccat gggacgcgcc gctgtgatcg gtagaatgac tcattacatc gcccccatca
    12061 gataaacaac agtgaacaca cagatccaaa ccacatccag gaagtgccag aacaggctca
    12121 ggcacatgat gcgggtacgg ttagtgctgg tcaggccgcg acgagcgatt tgtaccatca
    12181 gcaccgccat ccagataaga ccggaagtga cgtgcagacc gtgcgtgccg accagcgcaa
    12241 agaacgctga caggaagccg ctgcgatccg gacccatgcc gttaacaatc aggtgatgga
    12301 attcgtagat ttccatcccg ataaatccgg cgccaaacaa ccaggtcaac gccagccagg
    12361 agataacctg gcttttgttg tttttgtaca tggcgatagc cgccatgccg taggtgatgg
    12421 agctgaacaa cagcaagaaa gtttcaacca gaacgaacgg cagttcgaaa atgtccttac
    12481 ctgtcgggcc gcctgcggtg ccgttcacca gaacggcata ggtagcaaac aagatagaga
    12541 acagaatgca gtcgctcatc aggtagatcc aaaatccgaa gattttggtt ccgcctgcat
    12601 cgtggtgccc gtgttcgtgc gcgtggacag tcgcgtgcgt caaagtatca gttgccattt
    12661 ttcagccctg ccttagtaat ctcatcgaaa tgctggtttt ccagtttttc gatttctgcc
    12721 accggcacgt agtaatccac gtcctcgtcg aagcttttca cgatccaggt gatgatcatg
    12781 cctgcgaagc caacaatcgc cagccaccag atatgccaga tcatggcgaa accgaagatg
    12841 gtggagaaag ccgcaatgac gatacctgca ccgctgtttt tcggcatatg aatttcttca
    12901 tagtggtcag gctttttata cgcttcgcct ttctctttca tttcccagaa tgcatcacgc
    12961 tcgtgaacgt gcggcacaac ggcaaagtta tagaacggag gcggggaaga ggttgcccac
    13021 tccagcgtac ggccacccca cgggtcgcca gtcaggtcac ggttctggtc gcggtcacga
    13081 atagaaacgt acatctgaat aacgaggcag agaataccta gcgcaatcag tactgcaccg
    13141 ctggctgcaa tcatcagcat ggtgtggaac tgcgggtcaa tctgctggct caaacgacgg
    13201 gtcatgccca tgaagcccag cgcgtacagt ggcataaagg caacgaagaa gccgatgatc
    13261 cagaaccaaa acgcgcgttt accccaggtt tcgttcagtt tgaaaccgaa cgctttaggc
    13321 caccagtagg tcatccctgc gaagcagccg aagaccacgc cgccgatgat cacgttatgg
    13381 aagtgcgcaa tcaggaacag gctgttatgt agaacgaagt ccgcgcccgg tactgccagc
    13441 agcacgccag tcataccgcc caccgagaag gtgacgataa aaccgatagt ccacagcatc
    13501 gcagaatgga acacgatgcg gccctgatac atggtgaaca gccagttgaa gatcttcacc
    13561 ccggtcggga tggcgataat cattgtggtg ataccaaaga aggcgtttac gttcgcgccc
    13621 gcacccatcg taaagaagtg gtgcagccaa acgatgaacg acagcacggt gatacagacg
    13681 gttgcccata ccagcgaggt ataaccaaac agacgtttac gcgagaaggt tgccgcaatt
    13741 tcggagaaca caccgaaaac aggcaggatc aggatgtaaa cttccgggtg gccccaggcc
    13801 caaatcaggt tgatgtacat catcatgttg ccacccatat cgttggtaaa gaaatgggtg
    13861 cccagatagc gatccagggt caacaacgcg acggtaaccg tcagaattgg gaaggaagca
    13921 ataatcagta cgttcgcgca cagtgatgcc caggtaaata ctggcatctt gaacatggtc
    13981 atgcccggtg cgcgcatctt cagaatggta acgaagaagt tgataccggt aagcgtcgta
    14041 cctataccgg atagctggag actccatatc cagtaatcga caccgactcc cggactgtac
    14101 tctattcccg atagcggtgg ataggccagc cagccggtct gcgcaaattc gcccacgccg
    14161 agagaaacgt taaccagaat cacaccaaca acggtaaacc agaagcttaa gttgttgagg
    14221 aacgggaacg caacgtcacg cgcgccgatc tgcagcggaa ccaccaagtt catcagaccg
    14281 ataacgaaag gcatcgccac gaagaagatc ataatcacgc cgtgcgcggt gaagatctga
    14341 tcgtagtggt gaggtggcag gaagcccgct tcgcccgccg aggcaagagc ctgctggcta
    14401 cgcatcataa tggcgtcagc aaaaccacgc agcaacatca caatcgccac gatgatatac
    14461 atgataccga ggcgtttatg gtcgacggag gtcagccact ctttccacag gtaggtccac
    14521 ttaccgaagt aagtgatcag gccaacgagc gccagacctc ccaaaataat gccagcgatc
    14581 gtaaccatga cgataggttc atggaacggg actgcatcaa gtgataattt tccgaacatc
    14641 tttattcttc ctcaacccct ttaatgggcg gattccgcgt ggctcatgtc catgccttcc
    14701 ataccttcgt gtgcgctgtg ctcaccttct ggctgggtca tgtccatgct cttaccgtga
    14761 gccataaact tgttaattac atcggcaaac aagtttggtt tcacgttgga gaaatattcc
    14821 acctggttgt attcgctagg cgcggccagt ttttcgaacg ctgccatgtc agacatggag
    14881 ttcggcgact gtttcgcttt tgcgacccac tggtcgaatg cggcgcgatc cggtgttgca
    14941 atagctttga acttcatgcc tgagaagccc gggccgctgt agctggcgga gataccgtca
    15001 taagtgccgg gttcgttggc gatcagatgc aggcgagtct gcataccggc catggcataa
    15061 atctggctac ccagacgcgg aatgaagaag gagttcatca cggagttgga ggtcactttg
    15121 aagtacaccg gagtgttcgc cgggaaagcg atttcattca cggtagcaaa gccctgttcc
    15181 gggtagatga agaaccattt ccagtccatg gaaaccactt cgatggtaat gggcttctcg
    15241 tcgtgtgcca gcggcttgct aggctcaaga gcgtgagtgg ttttccaggt cagtaccgca
    15301 aggaagatga tgattaagat aggtaccgtc cagaccacag cttccacttt attggagtgt
    15361 gaccagttcg ggctgtactt agcatcttta ttgctcgcac ggtacttcca ggcgaaacca
    15421 acagccatca agattgcggg aataacgaca atcaacatca ggccaaatgc cgtcagtatc
    15481 agtgaacgtt gctccagacc aatctgtcct ttgggatcta acagcgcaga attacagcca
    15541 ctgagcaata cagtgcctgc aaataatgac aaccatccca aacttttatt gtatttcctg
    15601 agtctcattt aacgacctca attccacggg atctggtggc gtttaaaatg tgtgggcatt
    15661 ttacgggaag gttacattac tgtaaacatg attaaattcc tgttacttgg atttggctgt
    15721 caggtcacat atgttgcaaa acatatcagg ttgattttaa gaagctgttg caaaagggcg
    15781 ttataacaaa aggggagtgc agaagaagtc tggacgggca attggtataa tcaatgcgaa
    15841 ataaaacaat tattta
//
LOCUS       DAZFDC010000109        15840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFDC010000109 DAZFDC010000000
VERSION     DAZFDC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.109"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            122..724
                     /gene="ais"
                     /locus_tag="ACHJ48_003587"
     CDS             122..724
                     /gene="ais"
                     /locus_tag="ACHJ48_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO4709067.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLGRLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(763..1188)
                     /gene="nudI"
                     /locus_tag="ACHJ48_003588"
     CDS             complement(763..1188)
                     /gene="nudI"
                     /locus_tag="ACHJ48_003588"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO4709068.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            1467..2009
                     /gene="yfaZ"
                     /locus_tag="ACHJ48_003589"
     CDS             1467..2009
                     /gene="yfaZ"
                     /locus_tag="ACHJ48_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO4709069.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2109..3311
                     /gene="yfaY"
                     /locus_tag="ACHJ48_003590"
     CDS             2109..3311
                     /gene="yfaY"
                     /locus_tag="ACHJ48_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO4709070.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTLALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3531..4313
                     /gene="yfaX"
                     /locus_tag="ACHJ48_003591"
     CDS             3531..4313
                     /gene="yfaX"
                     /locus_tag="ACHJ48_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO4709071.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4328..5533
                     /gene="rhmD"
                     /locus_tag="ACHJ48_003592"
     CDS             4328..5533
                     /gene="rhmD"
                     /locus_tag="ACHJ48_003592"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO4709072.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQLRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5590..6879
                     /gene="yfaV"
                     /locus_tag="ACHJ48_003593"
     CDS             5590..6879
                     /gene="yfaV"
                     /locus_tag="ACHJ48_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4709073.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKLEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLLGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6905..7699
                     /gene="yfaU"
                     /locus_tag="ACHJ48_003594"
     CDS             6905..7699
                     /gene="yfaU"
                     /locus_tag="ACHJ48_003594"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO4709074.1"
                     /translation="MINHPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDG
                     EHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQA
                     RQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDE
                     ILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPD
                     MAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7740..8666)
                     /gene="rpnE"
                     /locus_tag="ACHJ48_003595"
     CDS             complement(7740..8666)
                     /gene="rpnE"
                     /locus_tag="ACHJ48_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO4709075.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGLQEGMHEQAI
                     KIALRMLEQGFDRDLVLAATQLSEADLAANNH"
     gene            complement(8859..10049)
                     /gene="glpC"
                     /locus_tag="ACHJ48_003596"
     CDS             complement(8859..10049)
                     /gene="glpC"
                     /locus_tag="ACHJ48_003596"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO4709076.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10046..11305)
                     /gene="glpB"
                     /locus_tag="ACHJ48_003597"
     CDS             complement(10046..11305)
                     /gene="glpB"
                     /locus_tag="ACHJ48_003597"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO4709077.1"
                     /translation="MRFDTIIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELPHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFTIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11295..12923)
                     /gene="glpA"
                     /locus_tag="ACHJ48_003598"
     CDS             complement(11295..12923)
                     /gene="glpA"
                     /locus_tag="ACHJ48_003598"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO4709078.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13196..14554
                     /gene="glpT"
                     /locus_tag="ACHJ48_003599"
     CDS             13196..14554
                     /gene="glpT"
                     /locus_tag="ACHJ48_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO4709079.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            14559..15635
                     /gene="glpQ"
                     /locus_tag="ACHJ48_003600"
     CDS             14559..15635
                     /gene="glpQ"
                     /locus_tag="ACHJ48_003600"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4709080.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         3680 a         3867 c         4278 g         4015 t
ORIGIN      
        1 ttgaggcatt ttgccagcat cgtaggattt ttaatattac accaactgct aattatcgcc
       61 agaaatgtcg ctcaatttca cggtaattgt ctggttgcgc ttgtctatag gtggagttta
      121 cgtgttagct ttttgccgct catcattgaa gtcaaaaaaa tatttcatca ttttactggc
      181 gctcgctgca attgccggac tgggtactca tgccgcctgg agtagcaatg gtttgccacg
      241 tatcgacaat aaaacgctgg gcagactggc acagcagcac ccggttgtcg ttttgtttcg
      301 ccatgctgaa cgttgcgatc gttcaaccaa tcaatgcttg tcagataaaa caggtattac
      361 ggttaaaggt acccaggatg cccgtgaact gggcaacgct tttagtgctg atatccctga
      421 tttcgatctt tattccagta ataccgtccg gaccattcag tccgcgacct ggttttcagc
      481 gggtaaaaaa ttgacggtag ataaacgact tcttcagtgc ggtaatgaga tttatagtgc
      541 gattaaggac ttacaaagca aagcgcctga taaaaatatc gttattttca cccataatca
      601 ttgcctgaca tatattgcta aagataagcg tgatgcgaca tttaaacctg attatctgga
      661 tggtttagtc atgcatgtgg aaaaaggcaa agtttatctg gatggggaat tcgttaatca
      721 ctaaattttc aatctgacag ccagtaatgg ctgtcatcat tgttacagaa gacctttcaa
      781 acgtaacgtt tttcgggtgg cgacattcaa atcatagtgc gccagatctt caggttttac
      841 ccacgcgtag tcctgaaact cttcgtttat tttcacttct cggttggcag aaacgcaatc
      901 aaaaatcagg taaatcatat aaatttcttc cttgcgacca tctgcatacg tcttggtgcg
      961 aatatcatcg ctgaaggtcc agggcgtgat ttctgtcaaa agcagctgtt ctcccagttc
     1021 ttcgcgaatt tcgcggcgta gagcctcttc aattcgttcg ccgggctcca cgccgccacc
     1081 cgaaagcgcc cattgaccgg ggaaaacgcc gcgatcgtcg gccattttac acagcaaata
     1141 agcaccatca ttttgaatca aagggcatac aatagtccgt tgtcgcacag gggtattcct
     1201 tataaataaa aagaaaccat gagaatacgg taactcagaa atatgtgaaa gtgacagtaa
     1261 tctcttttgc gatgataact gcacttatta ttatttttgt gtgtatcggt aaactgtgtt
     1321 tattttttcg gcgtttaatt atttaagaag tatttagtta caataagtga ctagatccct
     1381 tttttcgctc attgaatcct ctgcgccagg cagtacaatc ccgcagcttt gggaattttg
     1441 attaattgct tgtgggaaag gggataatga aaaaaattgc gctagcaggt ctggccggaa
     1501 tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag cggccaggcg ggtaaagaat
     1561 acaccaatat tggtgtcggt tttgggactg aatcgacggg cctggcttta agcggtaact
     1621 ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg gctggggttg aatctgcctc
     1681 tcgggccgtt aatggcgacc gttggcggaa aaggcgttta caccaacccg aattacggcg
     1741 atgaaggcta tgccgcagcg gtaggaggtg gtttgcagtg gaaaattggc aacagcttcc
     1801 gtttgtttgg cgagtattac tactctccgg attcactctc cagcggtatt aaaagttatg
     1861 aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc agtcagtatt gaggcgggtt
     1921 atcgctacct gaatctgtcg ggtaaagacg gtaaccgcga caacgccgtg gctgacggcc
     1981 cgtatgttgg ggttaacgcc agtttctgat cccttccggc gcggcattct gtcgcgccat
     2041 tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa caaaagcagg
     2101 agataaccat gttaaaagtg gaaatgctat ccaccgggga tgaagtgtta cacgggcaaa
     2161 tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg ttgccattat
     2221 ctcgccgcaa tacggtgggg gataaccttg atgacttagt caccattctt cgcgaacgta
     2281 gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg accgaccagc gatgatttaa
     2341 gcgcactcgc cgctgcgaca gcaaaaggtg aaggcctggt gctgcatgaa gcctggctca
     2401 aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc aaccgtaaac
     2461 aagcggagct gcctgccagt gctgaattta tcaataaccc ggtaggcacc gcctgtggtt
     2521 ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc cggcgtaccg tcagaattta
     2581 aggtgatggt cgagcacgaa atcctgccgc gtctgcgcga gcgtttttct ttaccacagc
     2641 cgccggtttg tctgcgttta actacctttg gtcgttcgga aagcgatctg gcacaaagcc
     2701 tggacactct acaactgccg ccgggcgtaa caatgggcta tcgctcctca atgccgatca
     2761 tcgaactgaa actcaccgga ccggcaagcg agcaacaggc gatggaaaaa ctgtggctgg
     2821 acgttaaacg tgttgccgga cagagcgtga ttttcgaagg caccgaagga ctgcccgcgc
     2881 agatcagtcg cgaattgcaa aaccgccagt tcagcctgac gttgagcgag caattcaccg
     2941 gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc actgctggcg tgtgaagtgg
     3001 ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg gattacagaa cggcgagcca
     3061 accattttgc cgggctggca ctggctgttt cgggtttcga gaacgagcat ctcaactttg
     3121 cgctagccac gccagatggc actttagctc tgcgtgtgcg tttcagcact acacgctata
     3181 gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgcta cgccgttggt
     3241 taaacggcca ggacattgcc agtgagcatg gctggattga ggttgttgag tccatgacct
     3301 tatccgtctg attccgccac tctgtgccgg tttactgcac cggcacattc cccctccggc
     3361 aaaacttgtc caacctgttg tactcaataa gaaaaaatcc gtgcgctgat tcacaaattt
     3421 gtgcgtaatt tctccagtcc gttggacgag gagcgggatt tgatgcgctt cacaatttta
     3481 ctccccctga gcaacgagac tgattccagt atctaagact ggagtccagt atgttggaat
     3541 caagcaaagt tcccgccctg acacgggcta ttgatattct caatttgatt gcccgcattg
     3601 gcccctgtaa tgctgcgacg atcattgaca cattggggat ccctaaaagc acggcctatt
     3661 tgctgcttaa tgaactcagg cgtcagcgtt ttcttagcct tgatcaccag gaaaattttt
     3721 gtttgtggac caagctggtg gagctttccg gtcatgcgtt aagcaagatg gatctccggg
     3781 aactggctcg accgcgtttg acgcaactga tggataccac cgggctgtta tgtcacctgg
     3841 ggattattga taacggcagc gcttattaca ttttgaaagt ggagtcatca gccactatca
     3901 gcgtgcgttc acatgaagga aaaagccttt cgctttatcg ctccggaata ggtaaatgcc
     3961 tgcttgcctg gcaacctgca actgtacagc aaagtattat cgaagggctg gtctgggagc
     4021 aggcgacacc cacaacgatt actcatccac agcaacttca cgaagaactg gcgcgtattc
     4081 gccgtcaggg ctggagctat gacaacggcg aagattacgc cgatgtccgt tgtgtcgctg
     4141 cgccggtgtt taacgccaat aacgagctta ccgctgcgat ctcggtggtc ggtacccgct
     4201 tacaaatcaa tgaagaatac cgtgattatc tggcgggcaa ggccattgcc tgcgccaggg
     4261 atatttcacg tctgttggga tggaaaagtc cctttgactt acaagcctca taacaatgga
     4321 gaacatcatg accctaccaa aaattaaaca ggttcgcgcc tggtttactg gcggtgcgac
     4381 agcagaaaaa ggcgctggcg gcggtgatta tcacgaccag ggggcgaatc actggataga
     4441 cgatcatatt gccaccccga tgagtaaata ccgcgattac gagcagttac gccagtcatt
     4501 tggcattaac gttcttggca cgttggttgt tgaagtcgaa gcagaaaacg gccagaccgg
     4561 attcgctgtt tcgacagccg gtgaaatggg ctgttttatc gtcgaaaaac accttaaccg
     4621 tttcattgag ggtaaatgtg tcagtgatat caaactgatc catgatcaaa tgctcaatgc
     4681 caccctgtat tactccggct ctggtggcct ggtgatgaac acgatttcct gtgtcgatct
     4741 ggctctgtgg gatctgttcg gcaaagtggt cgggcttccg gtttataaac ttttaggcgg
     4801 cgctgttcgt gatgagattc agttctacgc cacaggtgcg cgtccggatc tggcaaaaga
     4861 gatgggcttt atcggtggca aaatgccgac gcactggggg ccacatgatg gcgatgcggg
     4921 gatccgcaaa gatgccgcta tggtcgcgga tatgcgtgaa aaatgcggtg aggatttctg
     4981 gttaatgctc gactgctgga tgagtcagga cgtgaactat gcgaccaaac tggcccacgc
     5041 ttgcgcgccc tataacctga aatggatcga agagtgcctg ccgccacagc agtatgaagg
     5101 ttatcgcgaa ctgaaacgca acgcgccagt cggaatgatg gtcaccagcg gtgagcacca
     5161 cggcacactg caatcttttc gtacgctttc agaaaccggt atcgacatta tgcagccgga
     5221 tgttggctgg tgcggtggct taaccacgct ggtggaaatt gccgcaatcg ccaaatccag
     5281 ggggcaactg gtggtgccgc acggttcgtc tgtttactcc caccatgcgg tgatcacctt
     5341 cactaatacg ccattcagcg aattcctgat gactagtccg gattgttcaa cgatgcgtcc
     5401 gcagtttgac ccgattctgc tcaatgagcc ggttccggtg aatggtcgta ttcataaatc
     5461 agtgcttgat aaacccggtt ttggcgtcga actcaatcgt gactgcaatt tgaaacgccc
     5521 ctacagccac taatcactcc gggcgttgct gtgacaacgg taacgcccac atattctaag
     5581 gagcttttta tgagcaccgc tttgcttgac gccgtggtga agaaaaaccg tgtgcgttta
     5641 attccgttta tgttggcgct gtatgtgctg gcgtttctcg accgttcgaa tatcggtttt
     5701 gccaaacaga cctaccagat tgataccggg ttgagtaatg aagcttatgc gctgggagca
     5761 ggcattttct ttgtggtata tgcgtttctg ggtgttccgg cgaatctttt gatgcgcaaa
     5821 ctgggggcca gaacctggat tggtacgaca acactgctgt ggggatttct ttcggcagcc
     5881 atggcatggg ccgatactga agcgaaattt ctgatagttc gcactctgct tggtgctgcg
     5941 gaggccggat ttttccctgg tatgatttat ctcacctcgc aatggtttcc gcagcgtaat
     6001 cgcgccagca ttatggggct gttctatatg ggcgcaccgc tggcgttaac actgggatca
     6061 ccgctttccg gcgcgctgct ggagatgcat ggatttatgg ggcatcccgg ctggttctgg
     6121 atgtttgtga ttgaaggatt gttggcagtc ggcgctgggg tattcacatt cttttggctt
     6181 gatgacacac cggagcaggc acgttttctg agtaaactag aaaaaacgtt gcttatcaat
     6241 caactggcaa gtgaagaaca acagaaagtg acttctcggc tgagcgatgc gctgcgtaat
     6301 gggcgagtct ggcaactggc gattatctac ctgaccattc aggtggcggt ttacggatta
     6361 attttcttcc tgccgaccca ggttgcggca ttgctgggaa caaaagtggg ctttacagcg
     6421 tcggtggtca ccgccattcc gtgggttgcg gccttgtttg ggacctggct tattccgcgc
     6481 tattccgata aaaccggcga acggcgtaat gtcgcagcgc tgacattact ggcggcgggc
     6541 attggtattg gtctgtccgg gctgctttct ccagtactgg cgatcgtagc gctgtgtgtt
     6601 gcagccatcg ggtttattgc cgtgcagccg gtgttctgga cgatgccgac acaactgctt
     6661 tccggtacgg cgctggctgc ggggattggt tttgtaaacc tgcttggtgc agtgggcggg
     6721 tttattgccc cgatcctgcg cgtgaaagca gaaacgttat ttgccagcga tgcggcggga
     6781 ttactgacgc tggctgcggt ggcggtcatc ggttcgctga ttattttcac tctgcgtgta
     6841 aatcgcaccg ttgcgcagac cgacgtggca catcattaaa taggctaagg aacacgatga
     6901 acgcattatt aaccatccct ttaaagaacg tttacgcaag ggcgaagtgc aaattggtct
     6961 gtggttaagc tcaacgactg cctatatggc agaaattgcc gctacttccg gttatgactg
     7021 gttgctgatt gacggggagc atgcgccaaa caccattcag gatctttatc accagctaca
     7081 ggcggtagcc ccctatgcca gccaacccgt gatccgtccg gtggaaggca gtaaaccgct
     7141 gattaaacaa gtcctggata ttggcgcgca aactctactg atcccgatgg tcgatactgc
     7201 cgatcaggca cgtcaggtgg tgtctgccac gcgctatcct ccctacggtg aacgtggtgt
     7261 tggggccagt gtagcacggg ctgcgcgctg gggacgcatt gagaattaca tggcgcaagt
     7321 caacgattcg ctttgtctgt tagtgcaggt ggaaagtaaa acggcactgg ataacctgga
     7381 cgagatcctc gacgtcgaag ggattgatgg cgtgtttatt ggacctgcgg atctttctgc
     7441 gtcgttgggc tacccggata acgccgggca cccggaagtg cagcgaatta ttgaaaccag
     7501 cattcggcgg atccgtgctg cgggtaaagc ggctggtttt ctggctgtgg ctcctgatat
     7561 ggcgcagcaa tgcctggcgt ggggagcgaa ctttgtcgct gttggcgttg acacgatgct
     7621 ctacagcgat gccctggatc aacgactggc gatgtttaaa tcaggcaaaa atgggccacg
     7681 cataaaaggt agttattgat atcaaaggcc catggggatc ggctgtggga ctgtgttaat
     7741 tagtggttat tcgctgccag atcggcttcg cttagctggg tggccgcgag caccaggtca
     7801 cgatcaaagc cctgttccag catgcgcaag gcaattttaa tagcttgttc atgcatgcct
     7861 tcctgcaaac cttctaattt accttcctgc caaccaattt gatgtccttc ctgccgtagc
     7921 cgttcagcaa tagtcattaa tctctccttt tgtagtggtg aacgttcggc aatctcctga
     7981 ataaaacggg tgaaacggga ggtatcgccg cattgcagca gataattaaa cagtgtttgt
     8041 agctggctgt cattagtgaa gcctctaacc aaaagcgtgg tgatcctgtc gaccataccg
     8101 attaaatcgc ggtcgcgaat atgcttttga atcagttcaa gcagagctat acgccgatgt
     8161 tgcatgatct catcgtcagg tacgatggta atatccacca acggaaaagc ttcggtgtac
     8221 aactgccggg caagttgcgg atcgtcaaac tcatccagcc agttgagtga gtacgggtag
     8281 ggcgaggttt cgccatgata aaacagcaac ggcaccacca gcgggactct gtcatagcct
     8341 ttgtccaggt gacgctgcat ggcggcagtg gcatagcgca ttagccgaaa agccatattc
     8401 ttttctgcag agctttgatg ttcaatcacg cagtagatat agccgtcacc gtcgctggtt
     8461 tccacggacc acaaaacatc cgagtagtaa gcgcgtaaac ttttttcaat aaaactagtg
     8521 ggttccaggc gtaaggtttg caggttgcaa agcttgcgca gtggttctgg taaatgtatt
     8581 tcgagaaaat cccgtgcggt ttcgggtgtg aacataaagg ttttaaatac tgcatcatgc
     8641 ggggaggagg ttgttgattc ggtcatggtt gtccgtcacc tgatatttat gatgacgcga
     8701 cattacggtt atcccaggcc tttgacatct gccagtttca gcctttgcga gacgttttca
     8761 gaggaatttt cactgcgttg caacgcatac attgggcacg gaatcgtgta cgcaaaaatg
     8821 cccggtaacc acatgcttac cgggcatcag aaaggagttt aagccagcgc ctgggccagt
     8881 agcgtaatcg gatgttcgca gcgaagactt gtggacatct caatctgcca tttacaggtt
     8941 tcgcagtcgg tgaccaccag atctgcgccg ctttcttcta tctggcggaa cagtggtgcg
     9001 ccgatggctt gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg
     9061 cagcactggg aatccagcac cgttaactca agccccggga tgttacgcaa cagctccagg
     9121 gtgtagagcg tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc
     9181 ggcagcggtt tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtc
     9241 gccagttcga tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg
     9301 tcgcgcaggg caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc
     9361 acagcttcgc ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag
     9421 ccgttggcga tcagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta
     9481 cccattgcgt tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg
     9541 aagcagccgt gaaagaaagc gacctggtct ttatactgcg cttgctgagc cgccacgctg
     9601 cgataccagc gacgaaacgt gccgaaggag tatttcggta gcgtgcggcg atgatcgatt
     9661 tttaacgccg catcaagcaa ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt
     9721 ggtgcgaacg gcgtggaaac gctacccatc aggtcggtat ggcttaaaac aaaattgcgc
     9781 agcgacgggc gcgtggtgtc atatttcgcc cgtgcgcgct ggatgatatc gccaatcttc
     9841 acatcggacg ggcaggcgac ttcgcaacgt ttgcagttga tgcaatattt cagtgcctcg
     9901 tcatacagtg cgccatcttt taaacgcaga cgctcgccat ccggcccggc ttgttttggc
     9961 cctggataac cgggattcac ccggctcact gggcaggcgg tggtgcagac ggtgcactta
    10021 atgcagtttt cgaagctggt gtcattcatt gttggcctcc tgcgcgttgg gcaatctgtt
    10081 gagcggcatg taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat
    10141 caaatccgcc cagcaccgaa ccgatggtaa acaggttttc gatggtttgc cctgcctgcg
    10201 acgggcgtag cgtctgatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa
    10261 aatctccctt ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg
    10321 gctctcgaat gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga
    10381 gaaccgcgaa acgtggacgc agcgggatat cggcgtgatt gcgggtccag atttcattca
    10441 ctacgccatt tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct
    10501 gacgcacaaa ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca
    10561 gcgttggcaa aagcatcagt gaacaaggta gtttttcatt caaccaacgc cacagtttgt
    10621 catcagccag gccgaagcag gcgggcatca ggatcatttc gcaggtattg gcgacaggaa
    10681 taagcgcatc aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca
    10741 ccgcgcggaa ctcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg
    10801 cggtttcaac ggcaaggtcg agttcacgca acgaagctgc cgcaaggtgc gcctgaaaat
    10861 ccatcagtcc gctaattccc actacacata ttttcttcgc gggcagcggc cagacgggga
    10921 cttctggcga acttagccag gtagagcgca gagtgccgag cggcgtaatc cgctggtgag
    10981 gcagttctac gctgccctgc aattgcgcac cgctttcagc gattaatgcc tgcgcctggc
    11041 aagcgagatc gagcacgcgt tgtggcccga gaagagagta aggatgtgca ggtgcctgct
    11101 gacgcaaaga ttccagtcca ctgtgaatgt ctgtcacagc ttgaccatct ggcagatggc
    11161 tcagcaaatc cagcgaaccg gatgagaaat gcagcgcgct ttgaccacgc gtgacaatgg
    11221 cacagcgcag gccgtgtttt tgcagctgca ggccacagag taatccggcg aggccgccgc
    11281 ccataatgat agtatcaaag cgcatctttc tgctccttct ccagaccaca cagtccctga
    11341 taaacccagc gggtaaattc gctttcgcgc agtgcatctc cccaggcgat gggttgcacg
    11401 cctttccagc gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg
    11461 acgttaaaac gttgcagcag tccagcagcg cggcaggcgc agagttcgcc ctggcaggta
    11521 cccatcccca cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct
    11581 accgcgtact gcacttcacc cgcagttacc gcttcgcact cacataccag gctacggtgc
    11641 agacggcctt cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg
    11701 cgcagcgggg caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcctgtgaa
    11761 cccggaagtg ccagatcggc ggtcgtacag gggcgcgtgt tgcccagttt gcggcatacc
    11821 gcgtcggtag cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg
    11881 ataaatccgt ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg
    11941 ttacgtccgc tcgggtcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc
    12001 aaaatgcgcg ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca
    12061 acctcttctg ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg
    12121 gtgccaatca gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg
    12181 cagcggttga tcacatgctg attaatgcgg tgatccatga tcagcagcga tcctttcgcc
    12241 gggaacatgc gaatgcgcag atcggcatat tcggcaatgt gttgccccca gatcccagcg
    12301 gcattaacca cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca
    12361 cgaacaccgc acaccgtcgc gccttcacga atcagccccg tgacttcatg agcggtaagg
    12421 ataacagcac cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca
    12481 acggtgccat ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata
    12541 atgcgcgctt gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga
    12601 ataaaagtgg cctggaagga gaggtcatct tccggcaggg tgataaacag gccgttggtt
    12661 ggttcaacgc agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg
    12721 gccgattccg catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc
    12781 ccggtggcac cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg
    12841 gcacagtcgc gggcaattcc ggctcccgtt gcgccgccgc caatgataat cacgtcactt
    12901 gattgcgagt cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc
    12961 atagtaaaaa catgagttgt ttgatttcgc gcatattcgc tcataattcg aaagtgagac
    13021 gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt gtgcggcaat
    13081 tcacatttaa tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct
    13141 ctcactgaat caggctgtta atcataaata agaccacggg ccacggaggc tttcaatgtt
    13201 gagtattttt aaaccagcgc cacacaaagc gcgcttacct gccgcggaga tcgatccgac
    13261 ttatcgtcga ttgcgctggc aaattttcct ggggatattc tttggctatg ctgcttacta
    13321 tttggttcgt aagaactttg cgctcgctat gccttacctg gtggagcagg gattctcacg
    13381 cggtgattta ggttttgcgc tttctgggat ctcgatcgcc tatggatttt cgaaattcat
    13441 catgggttcg gtatcggatc gctcgaatcc gcgcgttttc ctgcccgcag gtttgattct
    13501 ggcggcggca gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt
    13561 gatgtttgta ctgttgttcc tctgcggttg gttccagggg atggggtggc cgccatgcgg
    13621 tcgtactatg gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa
    13681 ctgtgcgcac aacgtcggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg
    13741 gttcaatgac tggcacgcgg cgctctatat gcccgctttc tgcgccattc tggtggcatt
    13801 attcgccttt gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga
    13861 gtacaaaaat gattatccgg acgactataa cgaaaaagcg gaacaggagc tgaccgcgaa
    13921 gcaaatcttc atgcagtacg tgctgccgaa caaactgttg tggtatatcg ccatcgccaa
    13981 cgtgttcgtt tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga
    14041 ggttaagcat ttcgcgctgg ataaatcctc ctgggcctac ttcttctatg agtatgcggg
    14101 tattccgggc accctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg
    14161 ggcaaccggc gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa
    14221 cccggcaggt aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta
    14281 cggccctgtg atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg
    14341 aacggcagcg ggctttaccg gtctgtttgg ttacctgggc ggttcggtgg cggcgagcgc
    14401 gattgttggc tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg
    14461 cggcagcatt ctagcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca
    14521 tcaggaatta ctgcaaaaac gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc
    14581 ttagcatggc gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca
    14641 gcaacgaaaa aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc
    14701 tgccagcaaa agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga
    14761 tgaccaaaga cgaccatctg gttgttctgc atgaccatta cctcgatcgt gttactgatg
    14821 ttgccgatcg tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca
    14881 cgctggatga aattaagtcg ttgaaattta ccgaaggttt cgatattgaa aacggtaaaa
    14941 aagtgcagac ttatccaggg cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct
    15001 ttgaagaaga gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta
    15061 tctatccaga aatcaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa
    15121 aaacgctgga agtgctgaag aaatatggtt acaccggtaa agacgataaa gtttatttgc
    15181 aatgttttga tgctgatgag ctgaagcgta ttaagaatga gctggaaccc aaaatgggca
    15241 tggatctcaa tctggtacag ctgattgcct ataccgactg gaatgaaacg cagcagaaac
    15301 agccggacgg aagctgggtt aattacaact acgactggat gtttaagccg ggtgccatga
    15361 aacaggtggc ggaatatgca gatggtattg gtccggatta ccatatgttg attgaggaga
    15421 catcgcagcc gggtaatatc aaactcactg gcatggtgca agatgctcag cagaacaagc
    15481 tggtagtgca tccttatacc gtgcggtcag ataaactgcc tgaatacaca actgatgtga
    15541 atcagttata tgatgctctg tataacaaag cgggtgtaaa tgggttgttt actgatttcc
    15601 ctgataaagc ggttaaattc cttaataaag agtaatgctg ctactgcagg atggcgttca
    15661 tgcgccatcc tctgctaaag cgaaataatg ctactaccgg ttattctttt cattaaccgg
    15721 tgcgtcctgt aaaaaagatg ccaacataat tttcgtaaaa ctcattcgtt aaaaaagcta
    15781 acactgggca ttgttattct ttaaacagta tgaataggtc tcaataataa attgtaatgc
//
LOCUS       DAZFDC010000110        15454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFDC010000110 DAZFDC010000000
VERSION     DAZFDC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.110"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(85..366)
                     /locus_tag="ACHJ48_003601"
     CDS             complement(85..366)
                     /locus_tag="ACHJ48_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HFO4709081.1"
                     /translation="MNKYWLSGAVFLAYGLASPAFSSDTTTLTINGRISSPTCSMDVV
                     NNHLQQRCGQLLQRVETNYRASSTAKGVTTEVVAVGNNDKRKIVLNRYD"
     gene            complement(628..1737)
                     /gene="apbC"
                     /locus_tag="ACHJ48_003602"
     CDS             complement(628..1737)
                     /gene="apbC"
                     /locus_tag="ACHJ48_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO4709082.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1869..3902
                     /gene="metG"
                     /locus_tag="ACHJ48_003603"
     CDS             1869..3902
                     /gene="metG"
                     /locus_tag="ACHJ48_003603"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO4709083.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4043..4771
                     /locus_tag="ACHJ48_003604"
     CDS             4043..4771
                     /locus_tag="ACHJ48_003604"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFO4709084.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQIKSFAD
                     VAAAEKAKLKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IPVTDDINRFAFPHRSRPREINYLRKDGEIWKRIADNTRAYDPDNNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIVLFALQL
                     KYKPVKGAVTTIIPPNHEAESLPS"
     gene            6728..7852
                     /locus_tag="ACHJ48_003605"
                     /pseudo
     CDS             6728..7852
                     /locus_tag="ACHJ48_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7865..10850
                     /locus_tag="ACHJ48_003606"
                     /pseudo
     CDS             7865..10850
                     /locus_tag="ACHJ48_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            10860..14489
                     /gene="yehI"
                     /locus_tag="ACHJ48_003607"
     CDS             10860..14489
                     /gene="yehI"
                     /locus_tag="ACHJ48_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO4709085.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVISRQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKAREEYWRTLLQTLMASQPQLAAE
                     VMSWLSTQARAVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLARL
                     ELTPQVYWQPGERERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDVWPALEHLLKQGPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQGKSARA
                     VSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRLV
                     PTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPEA
                     LARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWGV
                     YPANEPRRLLNAFRVAAEGGFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLFA
                     DYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAFV
                     YDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFNLSEALSAP
                     DVIFH"
     gene            14551..14868
                     /gene="yehK"
                     /locus_tag="ACHJ48_003608"
     CDS             14551..14868
                     /gene="yehK"
                     /locus_tag="ACHJ48_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO4709086.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRSTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         4133 a         3843 c         3735 g         3743 t
ORIGIN      
        1 catttatggg atgaataaaa aagccaaaat cattcatagg aatgattttg gctttttaaa
       61 tatgcttaaa acggtgttgg caaattaatc gtagcgattt aacacgattt tgcgtttgtc
      121 gttattaccc acagctacca cttctgtcgt aacgcctttt gcagtagaag aagcacgata
      181 gttggtttca actcgttgta acaactgccc acaacgttgc tgaagatgat tattaacaac
      241 atccatactg catgtagggg aactaattct gccattgatg gttaatgttg tagtatcaga
      301 agagaacgcg ggtgatgcta gtccataagc aagaaaaaca gctcctgaca accaatactt
      361 attcattgaa cgttatccct gtagtaaagg ttatgccggg tagattgagt gcataacaca
      421 ctatagctgt acatccgcta cacagccacg agggatgata aagagacagt ggttgcacaa
      481 tcaatcgcct ttgtacagtt tcggactaag atccactgaa tattcgcagt attaaataag
      541 tgttcattaa tgcggcaaaa acaaatacac cgcatcaata taacatctct gcggtgtatg
      601 aagaataacc agaatggtta ataggcgtta gaccgcgcgg aaggaaatct cgcctggaat
      661 gacttcaccc tgccagtaga gctgagctgc aacgcggtca gccagctggc ggtagatagc
      721 ggtaaattcg ctctccggac ggctgataac cgttggcgtg cctttatcga gatcctcacg
      781 taaggagatg tgtaacggca tctgccccag cagctgtgta tggtattttt cagccagttt
      841 ctctgcacca ccggtaccga aaatcggttc atggtgacca cagttactgc aaatatgcac
      901 actcatgttc tcgacaatac cgagcaccgg aacctcgact ttctcgaaca tcacaatgcc
      961 ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg accacgactg caccagttac
     1021 tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg cctggcggca tatcaagcac
     1081 cagatagtcg agatccggcc acaaggtttc ctgcaacatc tgcattaacg ccttgctcgc
     1141 catcggtcca cgccacacca tcgcattgtc gtcagtgacc agataaccaa ttgaattggt
     1201 tgccaggcca tgagacataa tcggtgccat atgggtaccg tccggcgaag ttggacgctg
     1261 attttctgcg cccagcatgg tgggaattga tgggccgtag atatcggcgt ccagaatgcc
     1321 gactttcgcc ccttccgccg ccagtgccag cgccaggttt accgccgtgg aggatttacc
     1381 cacgccgcct ttaccggaac tgaccgcaat gatgttcttc acgccattaa tacccggctg
     1441 attcttcacg cgttttaagg tggcgatatt gtgcgacagt ttccagtcga tagctttcgc
     1501 cccggtgata cgcagcagtt cggcgctaca ttgctctttt aactcttcaa acgcgctatg
     1561 ccagacgaat ggcataacca gttccacatg caacgtgtcg tccatccagg cgacatggtg
     1621 caacgctttc agcgtggtga ggttatgctt cagggttggg tgctgaaaat tggccagtgt
     1681 cccggcgacc attgcgcgca aggcttctgg tgatttggcc tgggattgtt cgttcatccc
     1741 gactcctttt ttgtatagat aaaccatcag ctgatagttt acctgaagaa tatagagaag
     1801 tacttactta acattttccc atttggtact atctaacccc ttttcactat taagaagtaa
     1861 tgcctactat gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg ccgtacgcta
     1921 acggctcaat ccacctcggc catatgctgg agcacatcca ggctgatgtc tgggtccgtt
     1981 accagcgaat gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc cacggtacac
     2041 cgatcatgct gaaagctcag cagcttggta tcaccccgga gcagatgatt ggcgaaatga
     2101 gtcaggagca tcagactgat ttcgcaggct ttaacatcag ctatgacaac tatcactcga
     2161 cgcacagcga agagaaccgc cagttgtctg agcttatcta tactcgcttg aaagaaaacg
     2221 gttttattaa aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc atgttcctgc
     2281 cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa tacggcgata
     2341 actgcgaagt ctgcggcgcg acctacagcc cgactgaact gatcgagccg aaatcggtgg
     2401 tttctggcgc taccccggta atgcgtgatt ctgaacactt cttctttgat ctgccctctt
     2461 tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt gcaggagcag gtggcaaata
     2521 aaatgcagga gtggtttgaa tccggtctgc aacagtggga tatctcccgc gacgcgccgt
     2581 acttcggttt tgaaattccg aacgcgccgg gtaaatattt ctacgtctgg ctggatgcgc
     2641 cgattggcta tatgggttct ttcaagaatc tgtgcgacaa gcgcggcgac agcgtaagct
     2701 tcgatgaata ctggaagaaa gactccaccg ccgagctgta ccacttcatc ggtaaagata
     2761 ttgtttactt ccacagtctg ttctggccag caatgctgga aggcagcaac ttccgcaagc
     2821 cgaccaacct gtttgttcat ggctatgtga cggtgaacgg cgcgaagatg tctaagtctc
     2881 gcggtacctt tattaaagcc agcacctggc tgaatcattt tgacgccgac agcctgcgtt
     2941 actactacac tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac ctggaagatt
     3001 tcgttcagcg cgtgaatgcc gatatcgtta acaaagtggt taacctggcc tctcgtaatg
     3061 cgggctttat caacaagcgt tttgacggcg tgctggcaag cgaactggct gacccgcagt
     3121 tgtacaaaac cttcactgat gccgctgaag tgattggtga agcgtgggaa agccgcgaat
     3181 ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct ggctaaccgc tatgtcgatg
     3241 aacaggctcc gtgggtggtg gcaaaacagg aaggccgcga tgctgacctg caggcgattt
     3301 gctcaatggg catcaacctg ttccgcgtgc tgatgactta cctgaagccg gtactgccga
     3361 aactgaccga gcgcgcagaa gcatttctca atacagaact gacctgggat ggtatccagc
     3421 aaccgctgct gggccacaaa gtgaatccgt tcaaggcgct gtataaccgc atcgatatga
     3481 agcaggttga agcactggtg gaagcgtcta aagaagaagt gaaagcagct gccgcgccag
     3541 ttactggccc actggcagat gatccgattc aggaaaccat cacctttgac gacttcgcca
     3601 aagttgacct gcgcgtggcg ctgattgaaa acgcagagtt tgttgaaggt tctgacaaac
     3661 tgctacgcct gacgctggat ctcggcggtg aaaaacgcaa tgtcttctcc ggcattcgtt
     3721 ctgcttaccc agatccgcag gcactgattg gtcgtcacac cattatggtg gctaacctgg
     3781 caccacgtaa aatgcgcttc ggtatctctg aaggcatggt gatggctgcc ggtcctggcg
     3841 ggaaagatat tttcctgcta agcccggatg ccggtgctaa accgggtcat caggtgaaat
     3901 aatccccctt caaggcgctg catcgacagc gccttttctt tataaattcc taaagttgtt
     3961 ttcttacgat tttgtctctc tctaacccgt ataaatactg gtagcatctg cattcaactg
     4021 gataaaatta cagggatgca gaatgagaca ctttatctat caggacgaaa aatcacataa
     4081 attctgggcg gttgagcagc agggaaacga gctgcatatc aactggggca aagtcggcac
     4141 taacgggcaa agccagataa aaagttttgc ggatgttgca gcagcggaaa aagcgaaact
     4201 gaagcttatc gcggagaaaa cgaaaaaagg ctatgtggaa aatgcttcag caaacgtgca
     4261 tatccccccc atcaccaaag ccactcctga agttgaaact tcccctgaga gtaaaaacca
     4321 acgcccgtgg ctggctgatg atgcagttat tccggtaact gacgatatta accgatttgc
     4381 ttttcctcat cgctctcgcc ccagagagat caattatctg cgcaaagacg gtgagatatg
     4441 gaagcgtatt gcagataaca ccagggcata tgatcccgac aacaattacc gctcttatcc
     4501 tgaaaactgg cagcaggctt ttgctgagtt acaaaggcga attctggata atcaacagac
     4561 agggagtgcg caatctgatg cagcattgct ctggagtttt tggaactctt actctgcaga
     4621 tgaactggtc gatgacttag tcattcgctg tggtctggaa agcgccgttg aaatcgtcct
     4681 ttttgccctt caactcaaat ataaaccagt aaaaggcgca gtaaccacca tcattccacc
     4741 taatcacgaa gcagaatcgc tacctagtta gcatcaacgt ctatgtcatc atctttcact
     4801 cgcttcagaa gatgagtggc aacgctgtgt agataaagta ctcaccgcca ttccgacgct
     4861 atcccccgca cggcaacctt ttgcggcgtt actgctccct gaacgcccgg atatcgccca
     4921 tgctatagat ctacgctatg cagaccaaaa cgtcccggcg atgacctggt taagactggt
     4981 gataaccgat gatactgctc tgagtacagt ggaaaaatac gacttcccac cgttgtatcg
     5041 tgattttcgt aattttcgcg cttatctggc aatgttgttg gcaaataatg gggtgcgcgg
     5101 tgtaagccgg atacttcttg agtttacgga agaccattct gacaatccca cttatctctt
     5161 cgaacgcatc agcgagactg aaaatcttgt caaatggcta tggaaaacga atcacccgga
     5221 tgcgattcaa attctgatcc tcggtgtaat tggtaagaaa aagcacctgg aatacttaag
     5281 caaggccagt caaaagcatc ccgctgcggc tattgcggct tatgctactt tgctggcaat
     5341 acatgaagat aaagagtggc gtaaagcgct tgtcaaactg attaccgcca cgccagagtt
     5401 agtatgcgac gtcattccct gggttaatgc taaagcggca ggcatcttat ctgaatgtcg
     5461 tccgcagtca gtcgctgaag aatgtgaata tgccactgtg gatatgttgc cggagctgct
     5521 tgtttcgccg ccctggatga ccaaagagaa aaagaaaaat acgcctgtgt ttgatcttcc
     5581 agtgcttcca gttccttctg tttctgatgt cactccagag attaccaaaa aactgacccg
     5641 tacttacctt gtcacccact tccagcaaat agcccaacag caggcgacaa aacaaacatt
     5701 gtttaccgac ctgccgccga tcaaaaaagc aagctgggag aaacacttaa taccgcttac
     5761 accggagcaa caaatactgt ggcatcttgg ttttgaaaaa tggcgggaga gcggagaaaa
     5821 aatatacgaa aaaattccgg cgccacaaag cgccgttgat gccctgctac gcttcgattt
     5881 tccagcactc aatgctgaat ttgtgcatta tcataacaat gcatacaaaa gctggaatct
     5941 cattgccctc tgttacctgc ctggtcaaca ggccatttct tttcttaacc agatagtcaa
     6001 agaagataac tactctggtg agggaaatat tctggcaatt tttggtagcg ccgccatacc
     6061 tgcatttatg gcctgcctgc aacgtgatcc acgacgtctg tgcttcttcc cctttttcct
     6121 cggtgtcagt gaactggcct taccgatggc gcaacaatta cagaaaaaaa tgtcttatga
     6181 agatgcgcga aactggctaa ctgattatcc acgccatgcc gccgccgggc tgcttcctgt
     6241 ggccctgggc aaaaaaggaa aagatcgtga ttgtgcccgc caggcgcttc gtctgctggt
     6301 gaatttaaac cagcgagaaa cgattgagga aatcgcgcaa ggatataatc aacctgatgt
     6361 tttagctgcg ctggcaacat tgttcgatag cgacccactg gaagaatatc ccgctaaaat
     6421 cgccccactg cccggctttt atcagttcac cttgtggcgc agaccacggc ttaaaagtaa
     6481 caacctgcct ctgtcagatg acgctatgcg ccacctcggc actatgctga gctttcctcg
     6541 cgacattacc gcctacgctg ggctggatat cattagagaa atcttcaccc gcgaatcact
     6601 ggctgaattt ggctgggatc tgtatcccgc ctggacagaa gctggcgcac ccgcaaaaga
     6661 aaactgggca tttacttcgc ttgggatttt aggcaatgac gacaccgcgc gtaaattaac
     6721 cccgcttatc cgcgcctggc ctggggaatc ccagcataaa cgggcggtgt ccgggctgga
     6781 tgtattagct gatattggca gcgatgttgc gctaatgctg cttaatggca tcgcgcgaaa
     6841 aattaaattc aaagcattac aggaacatgc ccgcgagaaa atcaacatag ttgctgaaaa
     6901 ccgtgggctg actatggctg aactggaaga ccgcctggct ccagatttag ggcttgatag
     6961 cagtggctcg ctgatactgg atttcggccc ccgcaaattc accgttggtt ttgacgaaac
     7021 cttaaaacct gtagtgtgcg atgcaaacgg caaagtcctg aaagatttac ctaagccaaa
     7081 ccagagcgat gaaaaaactc aggcaactga cgcggttaat ctcttcaaac agttgaaaaa
     7141 agatgtacgc gccatagcca gccagcagat tgatcgtctg gaacaggcta tgtgccagcg
     7201 cagacgctgg acggcagagc agttccgcct gtttctggtg gagcatccgc tggtacgtca
     7261 cttaacccga cggctgttat ggggggttta taacgatgaa aacgccctta tcacctgttt
     7321 tcgcgtggca gaagacagca cttacagcga tgcgcaggat gagttattca cgctgccagc
     7381 aggaaacatc ggtattccgc atgtgctgga aatatcccct gaatccgccg ctgcattcag
     7441 gcaaatttac gctgattacg aactgcttcc ccctttccaa cagctcgagc gaggtagcta
     7501 tcaccttgct gataatgaac gtaatactca cgaactgaca cgctggcaag gacggctttg
     7561 ccaggccgga cgcattgtcg ggctggaacg cagaggctgg caacgtctgg aagaaagcgg
     7621 cagcgtttat gcgatgcgca aaacaacccc tcatggcgat ctcgaacttg aaacggaacc
     7681 tttttcatta atttatggtg aaacggggta tggcgaccag cacccggttg aaagcgtaaa
     7741 aatcacctcg ccagatgatc gctacggtaa acaatcctca ctcactttct ccatgctcga
     7801 cgacatcacc gccagcgagc tgattaacga cattgaatca ctgtttgatt aaaaggattt
     7861 aacaatgaaa acctatattt atcaggacga aaaatcacat aaattctggg cggtggagca
     7921 gcagggaaac gagctgcata ttaactgggg taaagtcggt accaacgggc aaagtcaggt
     7981 aaaaagcttt gcagatactg cggcggcgga aaaagcagca ctgaagctga ttgcggagaa
     8041 ggtgaagaag gggtatgtgg agcaagtaga cgctaacgct cactcacatg atgcctcagc
     8101 aaagaaagtc ctggtcacag aggaaaaagc ctcaataaat cgccaggcag caactaatgg
     8161 tttaccctgg ctggcagaca acgatcccat tattcttccc ccgaatattg cccgccatgc
     8221 cctcagccac cgcctttggc cgggagagcc agtaaaaaaa ccaaccaaac ccgagaaaca
     8281 aaatttaatt tcccgattgg caaatcgtac ttatcattat tataaaaatc tgactttcga
     8341 ttacagctca tgctctgatg aatgggctga tgcgattagc caggcaatca gcttaattaa
     8401 ggcccaaaag actgagatac ccgcgcgtac tatggcggta cttacttttc tggaaatatg
     8461 tttttgtcag ggctggaatg atgaaacctc atccgagttg ctggatgaaa ttgtaagcac
     8521 cttcggtatc gaatacgcaa ctgaaactgt catcttctgg tggcaaattg atttcgattt
     8581 tgactataaa gatgaacatt tgactttctt tattaattat gcagatagca gcaaggacag
     8641 ttatagaagg aataacgaca aatttagttt acgtctgcgt aaacatttat cactagcaga
     8701 agaagaagta tggcaaaatt gcattgcaaa attactcgtt gcgttaccag atatatcact
     8761 ctatcgccaa ccgttaattg ccattttaat gccagaaata cccgaagtgg cccatgagat
     8821 cgtacatcgt ttacaccagg tcgcagatgt tccacaactt gaaatgttga aactggtagc
     8881 gacagatcca ttcacgctcg aaatactgga gaactatcaa tacattgatg tatttaacac
     8941 ctatggtgcc agttggtcag ctacggtatt acgggagcaa ggtatagcgg cactggctcg
     9001 cctgactccc tatgctgaag aagataattg cggtgacgta ctgaaatgta ttaatcatcc
     9061 gttggccatt acgaataaaa agtgccgaaa attttccttc tgcaacgata gtggcattag
     9121 ccgaagcatt ggtaaaaaaa gatgataaac gcaaacatac attgctggtt tcattactta
     9181 atgcaaatcc tttatttgtc gaaaaattag tcatcggatt atcgtcgcaa gcggcaaatt
     9241 tattgcaaca atgtcagcaa ttgctgaata gtcgcaatga agattttgca aactcagaaa
     9301 ttctaccaat ggtactagtc tcgccgccct gggaaactca aaataaaaat aacgacaaca
     9361 gtcctgtggg taaatacccg gcgaaaatcc cccctttacc gttgttttat caacctccat
     9421 tatggactcg cccgaaactc aaaaatagca gcaaggtact gccagatagc gcactccagc
     9481 acctcggtga aatgctccgc ttccctcagg aagaggccct gtatccggga ttattgcagg
     9541 tgaaagatgc ctgtaccgcc gactcactgg ctgaatttgc ctgggatctg tttaccgcct
     9601 ggctgactgc gggcgcaccg tcgaaagaga gttgggcgtt cactgcgtta ggcgttcttg
     9661 gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct ggtgaatccc
     9721 agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt gatatcgccc
     9781 tgatgcagct taacggtatt gcccagaaac tgaaattcaa agcattacag gagcgggcaa
     9841 aagaaaaaat tgccggcatt gccgaaagcc gcgaactcac ggtggcagag cttgaagatc
     9901 ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat ttcggctcac
     9961 gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat gtttctggca
    10021 gccgtctgaa agacctgccc aaaccaaaca aaagcgatga tgaatcacag gcgaacgatg
    10081 ctgttaaccg ctacaaactg ctgaaaaaag atgcgcgtac cgtcgccgcc cagcaggtag
    10141 caaggttgga atccgccatg tgcctgcgtc gccgctggtc gccagaaaac tttcagctct
    10201 tcctggttga acatccactg gtacgccact taacccgccg tctgatttgg ggcgtttata
    10261 gcaccgaaaa ccagctactg acgtgcttcc gcgtggcgga agataacagc tacagcaccg
    10321 ctgacgatga tcttttcacc ctgccggaag gcgatatctc tgttggcatt cctcacgttc
    10381 tggaaatatc accgacggat gctaccgcct ttggtcagct ttttgccgac tatgaactgc
    10441 taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc gagcgcaacg
    10501 ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggtcgggtc atggggctgg
    10561 cgaataaagg ctggataaag ggcaccccgc aggatggagg ctggatcggc tggatgatca
    10621 aacctctggg ccgctggtcg ttaatcatgg aaatcgatga aggttttgcg gtaggcatgt
    10681 cgccagccga actgagtgcc gagcaaatct taagcaagct gtggctatgg gaaggcaacg
    10741 cagaaagcta tggctggggg agtaattcaa cacaggaagc gcagttctcc gtactcgatg
    10801 ccatcaccgc cagcgagcta attaacgata ttgaagccct gtttgaataa ggaaagagca
    10861 tggacaagga attaccgtgg ctggcggata acgcccaact ggaactgaaa tataaaaaag
    10921 gcaaaacgcc gctcagtcat cgtcgctggc cgggcgaacc agtgtccgtt atcactggaa
    10981 gtctcatcca gacattggga gatgaattac tacaacaagc aggccagaag gaaaatatca
    11041 cctggaatta tgataaatgc tcactggagt ggcagtccgc catccagcag gcaatcaatt
    11101 tgactggcga gcataaaccg tcaatcccag cccttaccat ggcggctctg atctgtatcg
    11161 cgcaaaatga cagccagcag ttacttgatg aaatcgtgca acaagagggg ctggaatatg
    11221 cgacggatgt agtgatatcg cgtcagtgca ttgcgcggcg ttatgagagt gactcactgg
    11281 tcgtcacttt gcagtatcag gacgaggact atggctacgg ttatggctcc gcgacctata
    11341 acgatttcga tctccgtttg cgtaagcatc tgtcgctggc agaggaaagt tgctggcagc
    11401 gttgtgccga taaactcatt gccgcactgc caggaatacc caaaattcgc cgtcctttta
    11461 ttgcgctgat cctcccggaa aaacctgaaa tagccaatga actcgccagt cttgaaagct
    11521 cccggtcaag tctccattca aaggagtggt taaaggtcgt tgctactgat aatacggcgg
    11581 taaaaaaact cgaacgatac tggggcctgg acgtatttag cgatcgcgaa gccagctaca
    11641 tgtcacaaga aaaccgcttc ggctacgcgg cctgcgcctc ccttttgcgc gaacaaggac
    11701 tggcagccgt tccgcgcctg gcaatgtatg ctcataaaga agattgcggc agtctgctgg
    11761 tacaaattaa ccatccgcaa gtcatccgca ccttgctgtt agttgctgat aaaaacaaac
    11821 ccagcctgca acgtgtggct aaatacagta aaaacttccc ccatgcgacg ctcgccgcac
    11881 tggcagaact gctggcgtta aaagaaccac cagcccgccc tggttatcca atcatcgaag
    11941 acaaaaagct gcctgcacag caaaaagctc gagaagaata ctggcgtacg ctgttacaaa
    12001 cgctgatggc atcgcagcca caactggcag cagaagtgat gtcgtggtta agtactcaag
    12061 ccagggcagt gctgaagagt tatttatcgg cactgccaaa accggttatt gatggcaccg
    12121 ataacagcaa tctgcctgaa atcctcgttt caccaccgtg gcgtagtaag aaaaaaatga
    12181 cagctccacg tcttgatttg gcacggctcg aattaactcc gcaagtttac tggcaaccag
    12241 gcgaacgaga gaggcttgcc gccactgagt ctgcccgtta tttcagcacg gaatctcttg
    12301 cgcaacgcat ggaacaaaaa agtgggcgag ttgtattaca ggaactgggt tttggggatg
    12361 atgtatggct gtttttgaat tatatactcc ctggaaaact ggatgctgca cgcaattcac
    12421 tcattgttca gtggcattac taccaggggc gggttgaaga gatcctgaat ggctggaact
    12481 ccccggaagc acaattagca gaacaggcgc tccgcagcgg tcacatagaa gcgttaatta
    12541 acatatggga aaatgacaac tactcacgtt atcgtccgga aaagagtgtc tggaacctgt
    12601 atttattggc acagttgccg cgtgagatgg ctttgacttt ctggctgcgt atcaatgaga
    12661 aaaagcatct gttcgcggga gaggactatt ttctcagtat cctcggattg gatgcgctac
    12721 caggtctgct gttggctttt tcacatcgtc caaaagaaac atttccgtta attttaaatt
    12781 tcggcgcaac agaactggcc ctgcccgttg cccgcgtctg gcgacgtttc gcggcgcagc
    12841 gtgatctggc tcgccagtgg attttacaat ggccggaaca tacggctact gcacttatcc
    12901 ctcttgtctt caccaaatct agcgataaaa gcgaagctgc attacttgcc ctgcgtttac
    12961 tttacgaaca tgggcatggc gaattgctac aaactgtagc caatcgttgg cagcgtacag
    13021 atgtatggcc tgccctggag catttactga aacagggtcc catggaaatt tacccggcac
    13081 gcattccaaa agcccctgat ttctggcatc cgcaaatgtg gtccaggccg cgccttatca
    13141 ctaataatca acctgttacc gatgacgctc tggaaattat cggcgaaatg ctgcgcttta
    13201 cccagggggg acgtttttat agcgggctgg aacaactgaa aacgttctgc cagccacaaa
    13261 cgctggcagc ttttgcctgg gatctcttca ctgcgtggca acaagctggt gcccccgcaa
    13321 aagacaactg ggcatttctg gcgttaagtc tctttggtga cgaaagcacg gcacgggatc
    13381 taacaacaca gatcctcgcc tggccacaag gcaaatctgc ccgtgctgtc agcggcctga
    13441 acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat atatcgcaac
    13501 gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggta gtcgcagaaa
    13561 atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg ggccttgatg
    13621 atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa
    13681 acctcaaccc ggttatcttt gatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc
    13741 gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa
    13801 aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca
    13861 cccgacgctg gtcgctggca gattttcatt ctctgtttgt taatcatccc tttacccgtc
    13921 tggttaccca gcgattaata tggggggttt atccggcaaa tgaaccgcgt cgtttactca
    13981 acgcctttcg tgtggccgca gagggggggt tctgcaatgc gcaagatgag ccaattgacc
    14041 tgcctgcgga cgctctgatt ggcattgccc acccgttaga aatgacagca gaaatgcgca
    14101 gtgaatttgc acagcttttt gccgattacg aaattatgcc gccttttcgc cagttggcgc
    14161 gccgcacggt gctgctcaca cctgatgagt caaccagtaa cagcctgact cgctgggaag
    14221 gtaaatccgc taccgttggg caacttatgg gaatgcgata caaaggctgg gagtcaggtt
    14281 atgaggacgc atttgtctat gacctgggtg agtaccggct ggtccttaag ttttcacccg
    14341 gttttaacca ctacaatgtt gatagcaaag cgttaatgag cttccgttct cttcgagtgt
    14401 accgtgacaa taaatccgtc acttttgccg aacttgatgt gtttaatttg agtgaggcgt
    14461 taagcgcacc tgacgtcatt ttccattaaa aacacagcgg gcagtgatgc aactgcccgt
    14521 tatcaacacg acttaccagc ggggaaagcg atgatcgtgc aaaaagagct ggttgctatt
    14581 tacgattatg aggtccctgt acctgaagat ccgttttcct tcagacttga gatccataaa
    14641 tgctctgaat tatttacagg ttccgtctat cgactggagc gattccggct acgttcaaca
    14701 tttcatcaac gtgatcgaga agatgctgac ccgctaataa atgatgcgtt gatttatata
    14761 agagatgagt gtattgatga gcggaaatta cgaggtgaat cacctgaaac tgtaatagca
    14821 atttttaatc gtgaactaca gaatatattc aaccaagaaa tagaataata tactctagat
    14881 aattaaagtc gcagcacatg caacttgaag tatgacgagt atagctttct taaaggggga
    14941 aacggtattc ccccttagat gcgtttcaat ttgcccgtta cttcaacaaa tcaatcaacc
    15001 gtaacagagc cactaataac tccagtacgg caatgagcac ggttaacttt tgtgtcagcg
    15061 tgtccatccg ctctccttat taaggagcgc aacaccagca ccttaccctt acactgcctg
    15121 tcgatactgg tttacgtatg ttggtacgaa acgttgctct ccggatcagg gcaccgcctg
    15181 cggcaccacc cgtccggcag ccaggactta aaattggtcc gatccggcac aacctcccaa
    15241 cgggcaggat tataaatctc actcactgaa ttgcaatgga gtgtggatat ggttataatt
    15301 tattcgttgg tacgaaatgt tgcacaatca gcaagttagc gtcactgtag taaataaaag
    15361 aacctgcctc accagcaggt ttttttattt tctgcgatct actttccaga tatttttttg
    15421 ccaatagccc accataaata ttcacatccc ggtc
//
LOCUS       DAZFDC010000111        15408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFDC010000111 DAZFDC010000000
VERSION     DAZFDC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.111"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            105..353
                     /locus_tag="ACHJ48_003609"
     CDS             105..353
                     /locus_tag="ACHJ48_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO4709087.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            331..1461
                     /locus_tag="ACHJ48_003610"
     CDS             331..1461
                     /locus_tag="ACHJ48_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO4709088.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1507..2145)
                     /gene="ompW"
                     /locus_tag="ACHJ48_003611"
     CDS             complement(1507..2145)
                     /gene="ompW"
                     /locus_tag="ACHJ48_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO4709089.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2385..2474
                     /locus_tag="ACHJ48_003612"
                     /pseudo
     CDS             2385..2474
                     /locus_tag="ACHJ48_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2502..3245
                     /gene="yciC"
                     /locus_tag="ACHJ48_003613"
     CDS             2502..3245
                     /gene="yciC"
                     /locus_tag="ACHJ48_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO4709090.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASIRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3275..3814
                     /gene="yciB"
                     /locus_tag="ACHJ48_003614"
     CDS             3275..3814
                     /gene="yciB"
                     /locus_tag="ACHJ48_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO4709091.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3919..4317
                     /gene="yciA"
                     /locus_tag="ACHJ48_003615"
     CDS             3919..4317
                     /gene="yciA"
                     /locus_tag="ACHJ48_003615"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO4709092.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATDALFKYVAVDPEGKPRALPVE"
     gene            complement(4357..5076)
                     /gene="tonB"
                     /locus_tag="ACHJ48_003616"
     CDS             complement(4357..5076)
                     /gene="tonB"
                     /locus_tag="ACHJ48_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO4709093.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQQKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5300..5596
                     /gene="yciI"
                     /locus_tag="ACHJ48_003617"
     CDS             5300..5596
                     /gene="yciI"
                     /locus_tag="ACHJ48_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO4709094.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5897..7150
                     /gene="kch"
                     /locus_tag="ACHJ48_003618"
     CDS             5897..7150
                     /gene="kch"
                     /locus_tag="ACHJ48_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO4709095.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7205..7378)
                     /gene="yciY"
                     /locus_tag="ACHJ48_003619"
     CDS             complement(7205..7378)
                     /gene="yciY"
                     /locus_tag="ACHJ48_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO4709096.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7521..8981
                     /gene="cls"
                     /locus_tag="ACHJ48_003620"
     CDS             7521..8981
                     /gene="cls"
                     /locus_tag="ACHJ48_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO4709097.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFHSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKVYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9016..9345
                     /gene="yciU"
                     /locus_tag="ACHJ48_003621"
     CDS             9016..9345
                     /gene="yciU"
                     /locus_tag="ACHJ48_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO4709098.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9398..10402)
                     /gene="oppF"
                     /locus_tag="ACHJ48_003622"
     CDS             complement(9398..10402)
                     /gene="oppF"
                     /locus_tag="ACHJ48_003622"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO4709099.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10399..11412)
                     /gene="oppD"
                     /locus_tag="ACHJ48_003623"
     CDS             complement(10399..11412)
                     /gene="oppD"
                     /locus_tag="ACHJ48_003623"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO4709100.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11424..12332)
                     /gene="oppC"
                     /locus_tag="ACHJ48_003624"
     CDS             complement(11424..12332)
                     /gene="oppC"
                     /locus_tag="ACHJ48_003624"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO4709101.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12347..13267)
                     /gene="oppB"
                     /locus_tag="ACHJ48_003625"
     CDS             complement(12347..13267)
                     /gene="oppB"
                     /locus_tag="ACHJ48_003625"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO4709102.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13353..14984)
                     /gene="oppA"
                     /locus_tag="ACHJ48_003626"
     CDS             complement(13353..14984)
                     /gene="oppA"
                     /locus_tag="ACHJ48_003626"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO4709103.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3921 a         3766 c         3652 g         4069 t
ORIGIN      
        1 tatctggcaa aaactgatcc cgaatcaggc tgatttttaa tgttcaaccc cggtcgttgc
       61 ccaccagcta tagtggcggc gaccatgatt agcgaacgac gctcatggca agacttattc
      121 tgctcactga gtgggcaaaa gaggaattca gtgaaccggt cccaactccg agtacgttaa
      181 gtaaatacgc taaagccgga atgatatttc ctctccccaa aaaagttgga agacgctggc
      241 gagtggatcc gcaagctcgc tttgtcggaa tggtaaacaa gccggaggtg atcgccacag
      301 atcaccctgc tttgaagagg atactggaag atggcgcgcc cgcgaaaata taaaaccgat
      361 gttccgggat tatctccgta ttttgacaaa agaaataaca aagtttactg gcgttacagg
      421 catcccataa caggcaaaaa tcacggtctc ggcagtattg accagaaact ggcagaaact
      481 attgcagcag aagcgaacag ccgtcttgcc cggcagcaaa tggaacaaat gctcagtctg
      541 caggagaaaa ttattagtga taccggcggt tcatcaaccg ttaccatttt tctgaataat
      601 tacagaaaaa ttcaacagga aagatatgaa aacggcgaga tcaaactcaa cacgctgaaa
      661 cagaaagcgg cccctctcag ggtatttgat gaacgttttg gcaccagacc gttagatgcc
      721 ataaccgtaa aagatgtggt atcagtactg gaagagtaca aggccagagg acataacaga
      781 atgggacaaa ttttcaggaa agtactgatc gatgttttcc gggaagctca gcaaacgggc
      841 gatgtcccgc caggctttaa ccctgcagaa tcggcaaaaa aaccgcaggt gcggatatca
      901 agacagcgac tgacttttga tgagtggatg atgatttata acgcagcgga aaaggatggt
      961 tactttttac agcgcggtat gctgctggca ctgatgacag gccagcgcct ttcagatatt
     1021 tgcaaaatgc aattttcgga tatccgggat ggttatcttc atgtcgaaca gcaaaaaaca
     1081 ggaacccgga ttgccatccc tctggctctg cgttgcgata aattaaatct caccctggat
     1141 gatgtggtgt catcctgccg cgattgcgtt cttagtccgt ggctattgca ccaccatcac
     1201 gcgaaaggga cagctaagcg cggcgggatg gttaagccag caacattaac cgttgcattt
     1261 aaaaaagccc gggattctgt ggattacaac tggcgtgcta atggcacccc tccctctttc
     1321 catgagcaga gatctttatc agagcgattg ttcagagagc agggggttga taccaaaatt
     1381 ttgctaggcc attcgaatca aaaaatgacc gatatttaca acgacgcacg cggtaaggaa
     1441 tggaaaaaac tggtcatttg atgaccagtt ttgcagaggg gttttgcaga ggttttgcag
     1501 agaaaattaa aaacgatatc ctgcggagaa cataaacacc cacggatcca gtcgtaccga
     1561 gtcttttacg gtagtaacac cagatttgta cttagccgtg gtatcgatat ccatgtacca
     1621 cactgacatg ttaaccaacc agtcacggtt aatcagataa tcaaccccca cctgcccggc
     1681 agctccccag gaatctttca gactgagatc ggaaagcccc gcctctttgc catgatcgtt
     1741 aaatccatta tcaaagaagg tggtgtagtt aatacctgcc ccaacataag gacggaattt
     1801 gctgctggca tcaccaaaat accactgcgc catcagtgtt ggcggcagat gatgaacggt
     1861 tgcaatatcg ccggtcgccc gggtgccgat tttatggcgg aacggcgtcg ctgccagtaa
     1921 ttccacacca atgttgtcgg tcgccatata agtaaacgtc aggcccagtt gagtgttatt
     1981 ggtcacgctg aatccaccca gacttcctaa cgtaccacca gcaccttctg ttggacgtac
     2041 ggttgcagaa cctgcacgca taaaaaattc gcctgcttca tgcgcaaagg cactgccaga
     2101 gagaagagtt gttactgcca aagccgccac tgttaacttt ttcatatccg ctccgtcgtt
     2161 atggttataa aaacgtgacg aatataccta cattggagta acaaatgatc ctacatagat
     2221 cacattacat atgataattc accatttatt gatctggatt aatttagaag ttgcattgag
     2281 aatcctggtt aacgtgattt aaatcaattt ttaaatattt tcgctacgca aatattaact
     2341 cgttcgccac ttgccgcggt ttgcccacgc ctgaaggcca gcaattacct ccgctttaca
     2401 aagatatgct tttccataca agacctgatg ctgccacccg aggcttaacc agggtacaat
     2461 tgcccgctaa ttaacacctg cataaaactc aaggagagtg catgtctatc acggcgcagt
     2521 ccgtataccg tgacaccgga aatttcttcc gtaatcaatt tatgaccatt ctgttggtat
     2581 cgttgctatg tgcgtttatc acagtggtgt tagggcatgt tttctcaccc agtgatgcac
     2641 agcttgcgca gctcaatgac ggcgtgcccg ttagcggcag tagtgggttg ttcgacctgg
     2701 ttcagaatat gtcaccggaa cagcaacaaa ttttgctgca ggcttcagcg gcgtccactt
     2761 tttcaggatt aatcggtaac gccattctcg ccggaggcgt aatattaatt atccagctgg
     2821 tgtctgcggg tcagagagtc agtgcgctgc gagctattgg tgccagtgcg ccgatattgc
     2881 caaagttatt tattctgatt tttctgacta cccttttagt acagattggc atcatgctgg
     2941 tggtcgttcc gggaattatc atggccatat tactggccct ggcaccggtg atgctggtac
     3001 aggacaaaat gggcgttttt gcctcgatcc gtagcagtat gcggctgact tgggcgaata
     3061 tgcgtctggt ggcacccgca gtactgagct ggttgctggc aaaaacactg ttgctgcttt
     3121 ttgcctcttc ttttgccgca ttaaccccgg aaattggtgc cgtactggcg aacaccttga
     3181 gcaacctgat ttcagccata ttgctcatct atctgttccg cctgtatatg ttgattcgcc
     3241 aataacctta agcatttgat tacggaatcg taaaatgaag cagtttcttg attttttacc
     3301 gctggttgtc tttttcgcgt tttacaaaat ttatgacatc tatgcggcta ctgcggcgct
     3361 gatcgtcgcc acggcgattg tgcttatcta tagctgggtt cgctttcgta aggttgagaa
     3421 gatggccctg atcacttttg ttctggtggt cgtcttcggt ggcttgacgc tgttcttcca
     3481 caatgatgag tttattaaat ggaaggttac agtcatttat gctctgtttg cgggagccct
     3541 gttagtcagc caatgggtga tgaaaaagcc gctaattcag cggatgctgg gtaaagaact
     3601 cacgctgccg caaccggtat ggtcgaagct gaatctggcc tgggctgttt tctttatcct
     3661 ttgcggtctg gcaaacatct acatcgcatt ctggctgccg caaaatattt gggtcaactt
     3721 taaagtcttt ggcctgaccg cccttacctt aatcttcaca ttgttaagcg gtatctatat
     3781 ctaccgccac atgccgcagg aagataaatc ctaaccagac tgccagtcct ttggggctgg
     3841 cgctgtatct cctctgttct cctcaggaaa atcatagtag catcgcgcct gtgattttcc
     3901 ttttaagtcg gttttaccat gtctacaaca cataacgtcc ctcagggcga tcttgtttta
     3961 cgtactttag ccatgcccgc cgataccaat gccaatggtg acatctttgg tggttggtta
     4021 atgtcacaaa tggatattgg cggcgctatt ctggcaaaag aaattgccca cggtcgcgta
     4081 gtgactgtgc gggttgaagg aatgactttc ttacggccgg ttgcggtcgg cgatgtggtg
     4141 tgctgctatg cacgctgtgt ccagaaaggg acgacatcgg tcagcattaa tattgaagtg
     4201 tgggtgaaaa aagtagcgtc tgaaccaatc gggcaacgct ataaagcgac agacgcatta
     4261 tttaagtatg tcgcggttga tcctgaagga aaacctcgcg ccttacctgt tgagtaatag
     4321 tcaaaagcct ccggtcggag gcttttgact ttctgcttac tgaatttcgg tggtgccgtt
     4381 aattttaaac aggatattca ccacaatccc actgcctggc ttacccggct cataacgcca
     4441 tctgcgcatc gcatttttca cctcacgctc aaacatgttc gcaggcttgg ctgagaggat
     4501 ttgtacgtta tccacgcgac catccggcgt gacgtcaaat ttaactttaa cctgcccttc
     4561 aatgcgcaat gcctgtgctc gtgccggata ctgcggctga ttacggctta atgcgcgtgg
     4621 tcctgaagcc acactggtaa ccggcttgct ggttgcagcc gttgctgtac ttgatgtcgg
     4681 gcgtgccggt gccgtatttt caaacggtga tgccggacgc gactctacgg gtttgacatc
     4741 gcgtttttgc tgctcctgta cctttttcac cggcttcggt tttggcttag gtttcggctt
     4801 cggcttttca atgaccaccg gtgcttcttt tggcggttcg gggatcggct caggttccgg
     4861 ttctggctct accaccggct ccggtggcgg ctgaacggct tgtggcggtt cgagatcagc
     4921 aggcgcaacc atcgtgacag aaatcggctg cgcaggcgca ggtagttcaa taacctgatg
     4981 taccgaggta tagagcagac ccgccacaac agcaccatga atgcagaccg aaagtaacgt
     5041 cggccagggg aagcggcgag gtaaatcaag ggtcattgaa gtcataatca tttcagtaga
     5101 aaaaccaggt ctcgatttta aatgcaaata gcaatcatat tcaataaggc aagacgatca
     5161 ggatcagtga aaatgtcatt tttggcgtaa ttgaccttac tttatgcatg gtcttaacaa
     5221 agacatcatc tttccgttgc agatcttcgc cctttcaaat aacgtacttt acaactttcc
     5281 cgaacaagga gttgtgcccg tgttgtatgt tatttacgct caagacaaag ctgatagcct
     5341 cgaaaaacgc ctttccgttc gtccggcaca tttagcacgt ttacagttac tgcatgatga
     5401 aggtcgtttg ttgacagctg gtccaatgcc agcggtagac agtaacgatc caggggctgc
     5461 gggatttact ggctcaaccg ttattgctga atttgaatcg ctggaagcag ctcaggcctg
     5521 ggccgatgct gacccgtacg tagcggcagg agtctatgaa cacgtttcag tgaaaccatt
     5581 taagaaagtg ttctgagtag agatccaata tttaagggca cggtttttgc aaccgtgttc
     5641 ttgtttggcc tttccttata ggtcggtcat tgcattcacc accacaagca gataataact
     5701 ttatctgtat ttctttatat gatgtgagca tgttcaggaa aaatgccctt tcctgacgca
     5761 gagttgctca ctcaaaacct ttgattactg cggataaagt catctactat tgtttaacac
     5821 attcacgtgt cgcatgtatg ttcagaaaat gtgcacgtta agataattaa acaaaattgt
     5881 ggttacagag gtatcagtga gtcactgggc tacattcaaa caaacagcaa caaatttatg
     5941 ggtgacccta cggcacgaca ttctcgcgct ggccgtcttt ttaaatgggt tgcttatttt
     6001 taaaacaatc tatggtatgt cggtcaattt gcttgatatt ttccatatca aagcattttc
     6061 agagttggat ctctccttgc tggcaaacgc cccactattt atgctcggcg tctttcttgt
     6121 cctgaactcc attggcttac tgttccgggc aaagctcgca tgggcaatca gtatcatttt
     6181 gttgttgata gcgctaattt acaccctgca tttttatccc tggctgaaat ttagtattgg
     6241 attttgcatt tttacgctgg tgtttttact gatactgcgc aaagacttct cccacagtag
     6301 cgccgcagcc gggacaattt ttgcatttat tagtttcacg acattactgt tttactcaac
     6361 ctacggtgcg ctttatttaa gcgaaggttt taatccgcga atagaaagtt tgatgaccgc
     6421 gttctatttt tcgatagaaa ccatgtcaac cgtcggctat ggcgatattg tccctgtttc
     6481 tgaatcagca cgattgttca ctatttcggt cattatttcc ggcattaccg tttttgccac
     6541 atctatgacc tcaatttttg gtccgcttat ccgcggggga ttcaacaaac ttgtaaaagg
     6601 aaacaatcat acaatgcatc gtaaagatca ttttattgtt tgcggacatt cgattctcgc
     6661 cattaatacg atcctgcaac tgaatcaacg cggacaaaac gtaacggtta tcagcaactt
     6721 gcctgaagat gatatcaagc aacttgagca acgcttaggc gataacgctg atgttatccc
     6781 cggtgacagt aatgacagtt ctgtattaaa gaaagcggga atcgatcgtt gccgggccat
     6841 tctggcgctg agtgataacg atgcagataa cgcgtttgtt gtactctcgg caaaagatat
     6901 gagcagtgat gtcaaaacag ttctcgccgt cagtgatagc aaaaacctga ataagattaa
     6961 gatggtacat ccggatatca ttctctcacc gcaactgttt ggcagcgaaa ttctggcgcg
     7021 agttttaaat ggtgaagaga ttaataatga tatgctcgtt tcaatgttgt tgaactcagg
     7081 tcatggtatt ttcagcgata acgatgaaca agaaacgaaa gctgacagta aagaatcggc
     7141 gcaaaaatag agtttaaaga cggaagagta aacttattgg gcaccgcaat ggtgcccagt
     7201 acatttagat attgtagatc gcaaacaata acgagttacg ctgtttgttt agaatgcact
     7261 tcctgatgct gtggatacgc atatttcggc gcgactgccc ctcaagccaa cgcgatttac
     7321 gtcggctagc ctgacgctgc atgcgccagc gccccacttc cgttctactc cgcttcatgt
     7381 taactactct atgcaataac agaatggtca ttataccccc gcccccgacg ctgaccagtg
     7441 cttttcccgc gtttttattt gccagatgaa tccatatgcg taaactcata acaatgcgct
     7501 ttcaaaagga tttctaatct atgacaaccg tttatacgtt ggtgagttgg ttggccattc
     7561 tgggatactg gttgctcatt gcaggcgtaa ctttacgcat tctaatgaaa cgacgcgcag
     7621 ttccctccgc gatggcctgg ctgttgatta tttacattct gccgttagtc ggaattattg
     7681 cctatcttgc cgttggcgag ctccatttag gcaaacgccg cgctgagcgc gccagagcga
     7741 tgtggccttc caccgcaaaa tggcttaacg accttaaagc ctgtaagcat atctttgccg
     7801 aagaaaatag cagtgtcgct gcgccattat tcaagctttg cgagcgtcgt caggggatcg
     7861 ctggggtcaa agggaatcag ctacaactga tgaccgagtc agatgatgtg atgcaggcgt
     7921 taatccgcga catccagctc gcgcgccata atattgagat ggtgttttat atctggcagc
     7981 ccggcggcat ggcggaccag gtggctgaat cattaatggc ggctgcgcga cgcggcattc
     8041 attgccgatt gatgctcgac tccgccggga gtgtagcttt tttccacagc ccgtggcccg
     8101 agctaatgcg taatgccggt attgaagtgg tcgaagcctt aaaggtcaat ctgatgcgtg
     8161 tgtttttacg ccgtatggac ctgcgccaac atcgcaagat gatcatgatc gataattaca
     8221 tcgcgtacac cggcagcatg aatatggtcg accctcgcta cttcaaacaa gatgcgggcg
     8281 tagggcaatg gattgatctg atggcgcgta tggaaggccc catcgccacc gcgatgggga
     8341 ttatttattc ctgcgactgg gagattgaaa cagggaaacg tattctaccg ccaccacctg
     8401 atgtcaatat tatgccgttt gagcaggcca gcggtcatac cattcacaca attgcttctg
     8461 gccccggctt tccggaagat ctcattcacc aggcattatt gactgcggct tattcggcgc
     8521 gtgaatattt gatcatgacc acgccctact ttgtgccaag cgatgattta cttcatgcga
     8581 tttgcacggc ggcgcagcgc ggggtggatg tcagtattat ccttccgcga aaaaatgact
     8641 cgatgctggt cggctgggcc agtcgcgcat tctttacgga actgcttgct gctggggtta
     8701 aagtttatca gtttgaaggc gggttactgc ataccaagag cgtgctggtc gatggcgaac
     8761 taagtctggt tggcaccgtt aaccttgata tgcgtagtct gtggctgaat ttcgagatta
     8821 ccctggcaat cgacgataaa ggttttggtg cagaccttgc cgccgttcag gacgattata
     8881 tttcgcgttc acgtctgctc gatgcccgtt tatggctaaa acgtccatta tggcaacgtg
     8941 tcgccgagcg actgttttac ttcttcagcc cgttgctgta aaacgtcgcc aacagaggtt
     9001 aaacaggtag tgactatgga tatggatcta aacaatcgcc tgactgaaga tgaaacgctt
     9061 gagcaggcct acgatatttt tctcgaactg gctgccgaca atctcgatcc agctgatgtt
     9121 ctgctgttca atcttcagtt tgaagagcgc ggcggcgcgg agttattcga tccggcggaa
     9181 gactggcagg aacatgttga tttcgatttg aacccagact tttttgccga agttgtgatt
     9241 ggtctggcgg acagtgaaga tggtgaaatc aacgatgttt tcgcccgcat tctgttatgc
     9301 cgcgaaaaag atcacaaact ttgccatatc atctggcggg aataaataat cactttcggc
     9361 gtgaaaaagg gctgacagct gtcagccctt tttgtgttta aagcggatcg actttcaggc
     9421 aagaaacggc gtgtctgaaa ctcccctcca gcacaggacg tgttttggca cactccggac
     9481 cggcaatcgg gcaacgggta cggaaaacac aaccggaagg cgggttgatc ggcgacggta
     9541 attccccttc cagtaactgg atggttttgt tcttctccag atccggatca ggtatgggga
     9601 ctgccgacat caatgccctg gtgtaaggat gtagtggatt gtggtagacc tcatcatagg
     9661 tccccagttc taccgcatgg ccgagataca tcaccaacac acgatcggaa atgtgtttta
     9721 ccacggccag gtcatgagcg ataaaaatta atgacaatcc catctcacgt tgcaattgct
     9781 ggagcaggtt gaccacctgt gcctgaattg acacgtccag cgccgacacc ggctcatcac
     9841 agataatcag cttcggttca agaataagag cacgcgcaat cccgatacgc tggcactgcc
     9901 caccagagaa ctcatgcgga tagcggttaa tcaggttagg caataacccg actttcagca
     9961 tcatcgcctt cacgcgctcg cgaacttcct ggcgtgacat tttcggatga taagtacgca
    10021 gtggttcagc gatgatctcg ccgatggtca tacgcgggtt tagcgatgcc aacggatcct
    10081 ggaaaatcat ctgaatatca ctgcgaacgg cacgccattc atcgggtttc atgcccagca
    10141 actctttacc taaccaggca acatgaccgt cggtcgcttt aaccaggcca atgatggcgc
    10201 gagcaaaggt ggacttaccg catcccgatt cccctaccac acctaatgtt tccccttcat
    10261 acaggcgaag agttacacca tctacggctt tgagcgtttt cggcggttgc cagaaccact
    10321 gtttgccatc tttgatttca aagtgcactt tcagatcggc gatttcgagg aggacttttc
    10381 ttccttcggt tacagcattc ataacagttc ctccaccggt ttaaagcaag cacgcagacg
    10441 gccaggcgta aactcttcca gcggcggtgc gctactacaa atttccatcg catgcggaca
    10501 acgtggctgg aatgggcaac ctttcggtaa tcgcagcagg tttggcggat tacccggaat
    10561 agtcaacatt gtttcacctt ccgcatcgag acgaggcacc gcgttgagca aaccgataga
    10621 ataaggatga acgggttgat aaaagacatc gcgcgcgttg ccatattcca tcgtgcgccc
    10681 ggcgtacatt accagcactt tgtcgcagat ccccgccacc acgccaagat cgtgggtgat
    10741 cataatgatg gcggtattaa attcccgctt cagttcattc aatagcgtca tgatctgcgc
    10801 ctgtacggtg acgtccagcg cagtagtggg ttcatccgca atcagcagct taggtcgaca
    10861 tagcaatgcc atcgcaatca taactcgctg acgcatgccg ccagaaaatt cgtgcgggta
    10921 cattttcatg cgtttacgtg cttccggcat ttttaccgca tcgagcatcc gcaccgactc
    10981 ttcaaacgcc tcagctttgc tcatgttctt atgcagcatc agcacttcca tcaactgctc
    11041 accgacgcgc atatagggat tcaacgaagt cattgggtcc tgaaaaatca ttgagatttg
    11101 ttcagcgcgc agtttattga gttcacgctc tggcaaattg aggatttcac gcccattgaa
    11161 ggttgccgat ccgccaatgc gtccgttggc agccaacagg cccatcaacg caaatgcagt
    11221 ttgcgattta cccgaaccag actcacctac aatgcccagc gtttctccgg cacgtaggga
    11281 aaaatttaaa tcattgaccg ccgtgacgtc gccgtccggg gtactaaagg tgacacgcaa
    11341 atctttcacg ttcagcagtg cgtcagcctg ttgttgtgcg agcggcacag ttgcagtttc
    11401 aattacgctc atggctgcac tccttaacga tctttcgggt cgagggcatc acgcaagcca
    11461 tcgccgataa agttgaaaca aaacagcgtc accacgagga atcccgctgg gaacaacagt
    11521 aaccatggag agacttccat tgagttcgcg ccatcactca gcaatgcccc ccagctgctt
    11581 aacggctctt gcgtacccaa ccccaggaag ctaaggaaag attcaaagag gatcatgctg
    11641 ggcaccagta gcgatgcgta gaccaccacc acaccgagta cgttcggcac aatgtggcga
    11701 ataacaatgc ccgacgtcga tacaccgcca acttgtgccg cctcaataaa ctctttgcgc
    11761 ttcagactca gggtttgccc acgcacaata cgagccatat ccagccagga aaccatgcct
    11821 atcgccacga aaatcagcag gatgttttga ccgaaaaagg tcaccagcaa aatgacgaag
    11881 aacatgaatg ggaaggagtt gaggatttcc agcaaacgca tcattaccga atccacttta
    11941 ccgcccagat aaccggaaag cgaaccataa agtgtcccca cgaccactgc caccagtgcc
    12001 gcagcaacac cgaccatgag tgagatacgt ccgccaatcg caacgcgcac aagcaggtcg
    12061 cgaccggatg agtcagtacc aaagtagtga ccggactcca tatccggggc gctggacatc
    12121 atcgcccagt cagtatcgtc ataggcaaac tgcgaaagca tcggtgccag gattacaaat
    12181 aacgcgatca gcaccagcac tatcagactt gcaaccgccg cacggttatg cataaaacgt
    12241 cgacgtgcgt cctgccacaa gctgcgccct tcgacctcca gcttttcact gaaattttcc
    12301 agcgtctcgc tgtttttctt acttaacatc atcgcgagct ccagtatcag taacggattt
    12361 tcgggtcgat aaccgcatat agcacatcga caatggcatt aaacaaaatg gttaacgcac
    12421 caaccaggat ggtcaggctt aacactaagg aatagtcacg gttcaatgcg ccattaacga
    12481 acagttgccc aatccccggc aaaccataaa tggtttcgat aaccatagaa ccggtaataa
    12541 tgccgacaaa tgcggggccc atataggaga gcacgggtaa cagagcaggt tttaatgcgt
    12601 ggcgtaaaat gatccgccgc ataggtaacc ctttcgcccg ggcagtacga ataaagttgg
    12661 agtgtaatac ttcaatcata gagccacggg taatacgcgc aatactggcg atataggcga
    12721 gtgacaacgc caccatcggc aatatcatga atttaagcgc cccaccattc cagccaccgc
    12781 ccggcagcca atgcaaaatg atcgcaaata tcatgactaa taatggcgca accacaaaac
    12841 tggggataac aaccccggtc attgccagcc ccatcacggt atagtcccat ttggtgtttt
    12901 gttttaatgc ggcaataacg ccagcactaa cacccagtat taccgcaagg aaaaatgctg
    12961 cggctcccag tttggcagaa acgggaaaac tggatgcaac caggtcattg accgaataat
    13021 ctttatattt aaacgatgga ccgaaatcgc catgcgccag ttgtttcagg tagctgaaat
    13081 actgtgtcat gatcggatca ttaagatgat atttcgcttc gatattggcc attacttccg
    13141 gcggtaaagt acgttcgccg gtaaaagggc ttcccggcgc gaggcgcatc ataaagaacg
    13201 aaatagtaat aagaataaat agcgtcggaa tcgcttccag acagcgacgt agaataaatt
    13261 ttaacattgc cctaccttct ggcctgtgcc ttctgtaatg cgataaaaat cagacaccgt
    13321 ggagcaggac actcctgccc cacgtattgc catcagtgct tcacaatgta catattccgg
    13381 gtgtaggtat tatccagcgg atctttgccg gtataaccac caacccacgg tttcaccaga
    13441 cgcgcattca cgtagtaata aacaggaaca atggccgaat ccttatccag ctgttgttct
    13501 gctttagtgt acagagctgt gcgctgcgcc tcgtcagtca ctttcagcgt ttccgccata
    13561 atgctgtcaa aggccgggct cttataatgc gcggtattca tcgagctgtt cgacagcatg
    13621 gtgttcagga aggaagttgg ttcgttgtag tcagcacacc agcctgcacg ggccacatca
    13681 aaagtaccct ggtgacgggt gtcgaggaac gttttccact cctggttaac cagtttgacg
    13741 tttacaccaa tgtttttctt ccacaatgaa gaggcagcaa tcgccagctt tttatgcaga
    13801 tcggaggtgt tatacaacag gttgatggtc aacggtttgt ctgcggtata acccgcttca
    13861 gccagcagtt ttttcgcttc ttcgttacgt ttttcctggc tccagccaaa ccattccggc
    13921 tgagtcaatt ttgcgccatc agtatacggt ggagtgtaac cataggcggg catgtcgccc
    13981 tgcgctttca ctttattaac aatgatatcg cggtccatac ccagtttcag tgcggtacgc
    14041 acacgcacat cgttgaatgg cggtttctgg ttgttaattt cgtaataata agtgcacagg
    14101 tatggatcaa cgtgaacttc gtccgggatc tcttttttca gcttctggaa caattcgatc
    14161 ggcatgttgt tataagtcat gtcgatttca ccactacggt agcggttgac atcggtaact
    14221 tcagaagcaa tgggcaaata ggttacctgg ttaataacgg ttttcgcgtt gttccagtag
    14281 gttgggctgc gttcaagaac gattcgttcg tttacgaccc aatcttttaa ggtataggca
    14341 ccgttggtga cgatattacc aggctgggtc catttttcgc cgaatttctc gatagcggct
    14401 tttggcaccg gtgaagttga tgggtgaaca agtaatttat agaagtacgg aacgggttca
    14461 cttaaggtga cttctaatgt gtgatcatca atagctttca cgccgagatc ggtaatcggt
    14521 tttttccctt caagaatttc atcaataccg gcgatatgcc catattgcag ataactggca
    14581 tacggagaag cggtgttcgg atcaacagaa cgttgccagc tatacacaaa gtcttgtgct
    14641 gtgactggcg tgccatcaga ccatttcgca tctttacgca aatggaaggt ccagactttc
    14701 gcgtctttat tatcccagga ttcagcgacg ccaggtgccg gatggccgtc aagatcgctg
    14761 accagtaagc cttcaaacag gtctcggctg atattagact ccggaacacc ttcaattttg
    14821 tgcggatcta atgactgaac ttctgaacca ttgttacgta ccagcgtttg tttttccgcc
    14881 agtgtgacgc ctgcgggtac atcagctgcc agcgcgacat tccctgccat tagcgcagcc
    14941 agaacgccag ctgctactaa acttctcttg gtgatgttgg tcattgtttt ttggactccc
    15001 tcattataat tactggtatt aaccagcatg tgtaatcccg gattgggacc ctgcacaggc
    15061 tcggagactt tctgctgtca ggttacttta tcgttacgga ttgctatcac cgactttatt
    15121 tattctggtg attcagacga tcaccttatg tcgattcgta ctgtgtctcc cctgtcgaga
    15181 cacattattc tttcgcgacg ctctttaatt gttaattatt ctcattacca ataaagaatc
    15241 gcctgacggt tgcccggaaa gtaccaaatg cggttcttac tcgccaatac attttgcaaa
    15301 attgttaagc aattctcttt tattgaccgt atcagaatct atagcttgca tccatgtctt
    15361 aacaaacatc tttaaattca atcaaataga gattggttaa gcatgtcg
//
LOCUS       DAZFDC010000112        15311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFDC010000112 DAZFDC010000000
VERSION     DAZFDC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.112"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            453..1109
                     /gene="pphA"
                     /locus_tag="ACHJ48_003627"
     CDS             453..1109
                     /gene="pphA"
                     /locus_tag="ACHJ48_003627"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO4709104.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(1110..1301)
                     /gene="yebW"
                     /locus_tag="ACHJ48_003628"
     CDS             complement(1110..1301)
                     /gene="yebW"
                     /locus_tag="ACHJ48_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO4709105.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1406..1642)
                     /gene="yebV"
                     /locus_tag="ACHJ48_003629"
     CDS             complement(1406..1642)
                     /gene="yebV"
                     /locus_tag="ACHJ48_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO4709106.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1760..3199)
                     /gene="rsmF"
                     /locus_tag="ACHJ48_003630"
     CDS             complement(1760..3199)
                     /gene="rsmF"
                     /locus_tag="ACHJ48_003630"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO4709107.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLRLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRSGGTLVYSTCTLNREENESVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAASVGLNWDGNLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(3280..5913)
                     /gene="yebT"
                     /locus_tag="ACHJ48_003631"
     CDS             complement(3280..5913)
                     /gene="yebT"
                     /locus_tag="ACHJ48_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO4709108.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTNNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEACSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5882..7165)
                     /gene="yebS"
                     /locus_tag="ACHJ48_003632"
     CDS             complement(5882..7165)
                     /gene="yebS"
                     /locus_tag="ACHJ48_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO4709109.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7295..7792
                     /gene="msrC"
                     /locus_tag="ACHJ48_003633"
     CDS             7295..7792
                     /gene="msrC"
                     /locus_tag="ACHJ48_003633"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO4709110.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7889..8587
                     /gene="proQ"
                     /locus_tag="ACHJ48_003634"
     CDS             7889..8587
                     /gene="proQ"
                     /locus_tag="ACHJ48_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO4709111.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8607..10655
                     /gene="prc"
                     /locus_tag="ACHJ48_003635"
     CDS             8607..10655
                     /gene="prc"
                     /locus_tag="ACHJ48_003635"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO4709112.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10847..11728
                     /gene="htpX"
                     /locus_tag="ACHJ48_003636"
     CDS             10847..11728
                     /gene="htpX"
                     /locus_tag="ACHJ48_003636"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO4709113.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11774..13147)
                     /gene="yebQ"
                     /locus_tag="ACHJ48_003637"
     CDS             complement(11774..13147)
                     /gene="yebQ"
                     /locus_tag="ACHJ48_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4709114.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQILTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            13324..14115
                     /gene="kdgR"
                     /locus_tag="ACHJ48_003638"
     CDS             13324..14115
                     /gene="kdgR"
                     /locus_tag="ACHJ48_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO4709115.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14259..14498)
                     /gene="yobH"
                     /locus_tag="ACHJ48_003639"
     CDS             complement(14259..14498)
                     /gene="yobH"
                     /locus_tag="ACHJ48_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO4709116.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAELGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14658..14801
                     /gene="mgrB"
                     /locus_tag="ACHJ48_003640"
     CDS             14658..14801
                     /gene="mgrB"
                     /locus_tag="ACHJ48_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO4709117.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14876..15163
                     /gene="yebO"
                     /locus_tag="ACHJ48_003641"
     CDS             14876..15163
                     /gene="yebO"
                     /locus_tag="ACHJ48_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO4709118.1"
                     /translation="MNEVVNSGVMNIASLVVSVAVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
BASE COUNT         4044 a         3688 c         3903 g         3676 t
ORIGIN      
        1 tcgtatgcaa gaacgtgcca cggctggctg atggacgttc gatagcgcga gtttgaatga
       61 aaatcagccg gagatgattt tacataattg ctacggaatt attcaataca ggaattgctt
      121 gtgtatgcat ggattgacct gaaatattcc cgaaaatttc tctaaaaaac tcgaaaaaaa
      181 tggtaactaa ttgaatgtat taatatgtaa tagtacgtgt tagagattaa aagatgagca
      241 tgaatttatt taacacatta attctaaaag attttgttgt ttgttgacga atacaggaat
      301 cgtgttcggt ctctttttat ctgttaaaag ccagaagcat ttccttcgct gactttatag
      361 tcaaccataa cacacactct actgtctgag tccagcgttt tttaacattc ttgttaagat
      421 tatgtgatct ttagcgcggg aggaaaatat tgatgaaaca gcctgcgccc gtttatcaga
      481 gaattgcggg tcatcaatgg cgacatatct ggctttctgg cgatatacac ggttgtcttg
      541 agcagttgcg ccgcaaatta tggcattgtc gttttgatcc gtggcgagat ttacttatct
      601 cagtgggaga cgttatcgat cgtgggccgc aaagtttacg ttgtctgcag ttactggaac
      661 aacattgggt tcgtgcggta agaggcaatc atgaacagat ggcgatggat gcgctggcat
      721 cccagcagat gtctttgtgg ttgatgaatg gcggcgactg gtttattgcg ctggcagata
      781 atcaacagaa acaagcgaaa acggcgctgg aaaaatgtca gcatttgccc tttattcttg
      841 aagtacacag ccgcaccggc aaacatgtta ttgctcatgc cgattatcca gatgatgttt
      901 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaagtg
      961 aacgccaaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
     1021 tgcgccatcg cgtggatatt ggcaacctgc attatattga taccggcgct gtctttgggg
     1081 gcgaactgac tttcgtgcaa ttgcaataat taaaaatcac catactcctg tgctggtcgc
     1141 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catgacggat ccgttgatcg
     1201 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
     1261 ccgccatcca ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
     1321 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
     1381 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
     1441 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
     1501 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtctgattta caaggaatgg
     1561 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
     1621 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgccggtt gtggtacgac
     1681 cagtccgatc gcaccatttt tcagcctagt tgaggccgcc gaatgtgcca gtcaaaaagt
     1741 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
     1801 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
     1861 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1921 cgcgatacca ctcctccgct tcttgcggtg tcagttcaaa agcgttctcg ttgtcggggg
     1981 aggcaagggc aataaccgct tcatgctgcc agcgataacc tttgttgtgc gtttcggcaa
     2041 gtttaatccc caggcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggaa
     2101 acaaccacac ttctttgtcg cgctgccaga gtcgcaggtt tccatcccag tttaagccaa
     2161 cacttgcagc cgcctgacga atttgtgcgg cttcgcgatc tttcaccggg ctgaacggga
     2221 aattgcccac tttgtatttt ggggtgggta aggcgggaat ggcctgagtt ttacgcagac
     2281 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aagccttctt
     2341 cggtcagcgc tttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctactgcgt
     2401 cggggtaagt ctctttaagc cacaggcaaa cggattcgtt ttcttcccgg tttaaggtac
     2461 aggtcgagta aaccagtgta ccgccagagc gtaatgcatg aaaggcactg tcgatcagtt
     2521 cccgttgggt agctgcgatt tcctgattgc tttctggtga ccaatttttt agcgcatcgg
     2581 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcgaaca
     2641 tttctggcaa tgccgcacca aacacgcggc catcaaaatg tgtgagcgca acattactga
     2701 taccacagcg gctgatattg gcatgtaaca cttttacccg actggcggaa aactcattgg
     2761 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttg gagcctggcg
     2821 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     2881 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gcggtactac
     2941 ccaatggcaa tgcatcttca tcgtcgcgtt caatccagaa accttcttca caccacggaa
     3001 ttggcgtaag cgtccagcca taaggagcgg ttaatcgcag gaaatcagca acggaggttt
     3061 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     3121 caaaagagag cgtcgaaggc attgcttcgc gcatttgcgt cagaaaggcg tccgggaaat
     3181 aaacggtgtg ttgggccacg agcatatacc actggcaaaa aaaacaggcg cgcagtttag
     3241 cataaacgct ccggcgcagg cacgccggag cagtgggcat tatttgggaa gcgcagttcc
     3301 ccattcacgc cactctttcg gttcactttc ctgcaacagg aagtgcttgc cttcctgggc
     3361 tttcggtgcc agtggcgtac ccggaggcgt ggcgaaggcg atgccgccac ggataaactg
     3421 gttaaaggtg ccggttttca ctacgccgcc cgtcagacca aagtccagac tgtaacccga
     3481 tgccaaccag aagacggaat tgttacgcac caggtgttga tagcgtttac tgatgcgcat
     3541 cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt ccggtaaccg taccgacttc
     3601 cagaccacgg aacagcacag gcgtaccgat gcctaacgaa caggcttccg gcgcttcaac
     3661 aataatgctt aaaccatcca ggtaacgcga atcagtaatg gtggcctctt gtaattcaaa
     3721 gtcgcgacga ggattgccac ggcctggttc gacgttgata tacggctgga ggatagtatc
     3781 aagatgctca acgccagctg ccgaaatttg cggtgtgacc actgagaagc gcgtaccacc
     3841 gcgagcaaag gtctggacat attccggata gagcaccgcc tttgcctgta cttcattgcg
     3901 cgcggtaatc agatccagcg tctggatttg cccgatatca ataccgagat agcgaattgg
     3961 catcccgacc gccagttttc cggcatcgaa agcatgaagc gtaatctgcc cgccaaccgc
     4021 acgggccgct gtttcggaag catacagaat acgcttatcg cctttacgct gactggcgct
     4081 ggcaccgctg aggttatcga agctaatggc tccctttaat gctctggaga gtggggatgc
     4141 ctgtacggtc aggccactac cattcagctg aactttcgcc ccgccttctg cccagaacac
     4201 gctattgttg gtcagaaggt tgcgatactc cggcttaata tgcagatcga tatcaaacgc
     4261 gttagctcgc ggacgcacgg taataacttc accaacttca aatttacggt acagcactac
     4321 cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa ctcacggttg tggtgggtaa
     4381 atcgctaagg ctgttctcca gcgctttttc cagattggca tacagtggat agctggcttt
     4441 catctcacct ttatcgcccg gcagaatacg tatcccgcca ttgatccatt ctgaggcgct
     4501 ggcaccgaga aactcaacgc catccagccc caccttcaca tcgacacggc tgttgacgac
     4561 aaatttgcta tcgcctttta ccagttcgcg atgctgaggc tcaatggcga cggtaaaggt
     4621 aacgcctttg ctggtgagtt tacgatcaat aacctggcct acctgcacgc cgtgaagaat
     4681 gagcggctga ccagcatcaa taccgtaact ttccggtgct gtcagggtca gtgtcagaac
     4741 atcaggttca tgcagcagtg ctttttcgcc tggcacaaca acgaactctt tgcgtggctc
     4801 gccatcgcca ggcaccaact caaaggtttt tccggtcagc agggcgctga gattggcatc
     4861 gctaagggat aatttcgggt tgcgtaattc gatgcgggta ttttcccgta acagcgtaac
     4921 gacgctggga tcaacggtca tttctccggt gactttacca ccaggattta aatccagttt
     4981 agtcagctgt ccgacttcca gcccctgata cattaacggc gtcgagtcgg cggttaatcc
     5041 ggccccactc ggcagttcca gttttattat tacgccacgc tggctgtggg ccagatcttc
     5101 atacagacca aaggtatctt ccgcctcggc aggtttcgac tcttctggtg aatcgaaggc
     5161 aatcgcaccg ttaaccagtg ccgccagact ttccagtttc accttcgcgc cactgatact
     5221 gacgttggca tcaacgcctg aaacgttcca gaaacggcta ccttttttca ccaggtcggt
     5281 aaaacgccgc tcgatcagga catcaatcac cacgccttgt ttattgggat tgatggcata
     5341 gtcgtagact tttcccaccg ggatcttgcg gaaatagacc aatgaaccgc tgttcagcga
     5401 accgagatcg ggagcttgca ggtggatcat cagatcgccg ttatccagcc gatacttcgg
     5461 ttgggtatcg agcgcgacaa agtgatcctg ctctttacct ttacctggca tcatgccgat
     5521 atagttacca ccgacgaggg cgtccagccc ggagacacct gccaacgatg cttttggcgt
     5581 caccagccag aactgagtct cttcgcgcag cgcatctttc atatcggact tgatgctgac
     5641 cttgacttca atcttacgaa gatcgtcgct gaggctgata tcctgcactg ttccgacttc
     5701 aacgccctga taacgaacag gcgtacggcc cgggacaata ccatccgccg acataaagtc
     5761 gatggtgacg gtattacccc ggtcctgata actgtcccaa atcagccaac cggcaatcat
     5821 tagcgcgatg aacggcagca gccagaaagg tgagatacgg cgtttatttt taatctgcgc
     5881 ttcagtcgtc gaagcgggcg tttcctgact catgtgcatc ccaaagtaag cggctgtcca
     5941 gccattccac agcaagaata gtcaaaatta ccgctgcgcc gaaataaaac gcagccggtc
     6001 ccatagtaaa agcgaggatc tgatcgcgat taatcagcga catggttaaa gatatgacaa
     6061 acaggtcgag catcgaccag cgaccaatcc aggtcaccat ccgcagtaac agaatgcgtg
     6121 tgcgtaaacc ttgctggcat ttaaaatgaa tgctcaacag taaagtgaac atgacgatca
     6181 ctttagtaaa cggtaccaga atactggcga taaacacgat tcctgcaacc gcaatgttgc
     6241 tacttgccag cgacataatt ccggaaagaa ttgtatcttc ctgccgtcct ccgttcagat
     6301 aaatgataga aataggcaac aggttggcag gtaacaataa aacgattgac gctaacagcg
     6361 ccgcccagca tttttgcaga ctatgacggc gacgcaggcg tagcggaata tgacaacgcg
     6421 ggcagcgacc acgctgatca ggatagccgg taaaatggca cccaagacag acacgaagtt
     6481 tctcgtccct acgcgtagcg gggcgctgcg gataaaatcg ctcccacagc tcctcgacat
     6541 taagatgtga caacgtcacc gtcgttaaaa tcaccaacgc cacaaaagag aacaagccga
     6601 cacccgcctg gatatgggca taatcctgta cctttataga agcaacgcca atgccgacca
     6661 ggtagatatc gagcattacc cactctttaa gtcgctcaag catcagcagt accgggcgta
     6721 gattcattcc cagtcggtta ccaaaccata aataagctat ggaggtcacc agaatgagcg
     6781 gggcaccaat aacgcagaaa aagaccatcg accccgttat cgcatcgccc tgtttggtca
     6841 tttgccagat gccttgcata acgttggcgt caatacgaat gcctaacagc cagatatgca
     6901 acagcggttc gccccaggca aacggcatca gcaacagcat ggtgaaggcc attgccgcca
     6961 ggcgcgttag cgaccagtcg cgcccgtcac gaatttttgc ctgacagcgc ggacaatagg
     7021 cactttgatg agaatttatc tcgggcaggc taaacagcat gtcacattgc gggcaacgtt
     7081 ggtaatcacc acgcggcagt tcctcgccga ttgccctcac tgttatcttt tttgtcggcg
     7141 taatttgtgg tgtgttaaga gccatgacgt gccaaatgta ataagaatta acattaatct
     7201 taacccatga tgggaatgat cttgagcatt aacgcattta atgcttattt taataggctg
     7261 gcagttccag gtaagacaac caagtgatta tataatgaac aaaacagaat tttacgcgga
     7321 tttaaatcgc gactttaacg cgctgatggc gggagaaacc agttttctgg caacgcttgc
     7381 gaacaccagt gcgttgttat atgagcgtct cactgacgta aactgggcag gtttttattt
     7441 gcttgaggac gatacactgg tgctcggacc atttcagggc aaaattgcct gtgtccggat
     7501 acccgtcggg cgtggcgtgt gcggcactgc ggttgcccgc aatcaagtgc agcgtatcga
     7561 ggatgttcat gcgtttgacg ggcatattgc ctgtgatgcg gcgagtaatt ctgaaattgt
     7621 tctgccgctg gtggtgaaaa atcagattat tggtgttctc gacatcgata gtaccgtctt
     7681 cggtcgcttt acagacgagg acgagcaagg cttacgtcag cttgtggcac agcttgaaaa
     7741 agtgcttgca acgacggatt acaaaaaatt ctttgcgagc gtcgcaggat aatcaacgga
     7801 taacgtagca tttactgata gcgtcattat aatgacgcct gttcatgcct gcgcttgttg
     7861 gctacgtccg ttgtaatcag gaaatttcat ggaaaatcaa cctaagttga atagcagtaa
     7921 agaagtaatc gcgtttctgg ccgaacgttt tccccactgt ttcagtgcgg aaggtgaagc
     7981 gcgtccgctg aaaatcggta tttttcagga tttggtcgat cgtgttgccg gggaaatgaa
     8041 cctgagcaaa acgcaattac gatccgcttt acgtctctac acttcgagct ggcgttacct
     8101 ttacggtgtt aaacccggcg caacgcgtgt cgatcttgac ggcaacccat gcggtgagct
     8161 ggacgagcaa catgtagagc atgctcgcaa gcagcttgaa gaagcgaaag cgcgtgttca
     8221 ggcacagcgt gctgaacagc aagcgaaaaa acgcgaagct gccgcagctg ctggtgagaa
     8281 agaagacgca ccgcgtcgcg aacgcaagcc acgtccgacc acgccacgcc gcaaagaagg
     8341 cgctgaacgt aaacctcgtg cgcaaaagcc ggtagagaaa gcgccaaaaa cagcaaaagc
     8401 acctcgcgaa gaacagcaca ccccagtttc tgacatttca gctctgactg tcggacaagc
     8461 cctgaaggtg aaagcgggtc aaaacgcgat ggatgccacc gtattagaaa tcaccaaaga
     8521 cggcgtccgc gtccagctga attcgggtat gtctttgatt gtgcgcgcag aacacctggt
     8581 gttctgaaac ggaggccggg ccaggcatga acatgttttt taggcttacc gcgttagctg
     8641 gcctgcttgc aatagcaggc cagaccttcg ctgtagaaga tatcacgcgt gctgatcaaa
     8701 ttccggtatt aaaggaagag acgcagcatg cgacggtgag tgagcgcgta acgtcgcgct
     8761 tcacccgttc tcattatcgc cagttcgacc tcgatcaggc attttcggcc aaaatctttg
     8821 accgctacct gaatctgctc gattacagcc acaacgtgct gctggcaagc gatgttgaac
     8881 agttcgcgaa aaagaaaacc gagttaggcg atgaactgcg ttcaggcaaa ctcgacgttt
     8941 tctacgatct ctacaatctg gcgcaaaagc gtcgttttga acgttaccaa tacgctttgt
     9001 cggtactgga aaagccgatg gatttcaccg gcaacgacac ttataacctt gaccgcagca
     9061 aagcgccctg gccgaaaaac gaggctgagt tgaacgcgct gtgggacagt aaagtcaaat
     9121 tcgacgagtt aagcctgaag ctggcaggaa aaacggataa agaaattcgt gaaaccctga
     9181 ctcgccgcta caaatttgcc attcgtcgtc tggcgcaaac caacagcgaa gatgttttct
     9241 cgctggcaat gacggcgttt gcgcgtgaaa tcgacccgca taccaactat ctttccccgc
     9301 gtaataccga acagttcaac actgaaatga gtttgtcgct ggaaggtatt ggcgcagtgc
     9361 tgcaaatgga tgatgactac accgttatca attcgatggt ggcaggtggt ccggcagcga
     9421 agagtaaagc tatcagcgtt ggtgacaaaa ttgtcggtgt tggtcaaaca ggcaagccga
     9481 tggttgacgt gattggctgg cgtcttgatg atgtggttgc cttaattaaa gggccgaagg
     9541 gcagtaaagt tcgtctggaa attttacctg ctggtaaagg gaccaagacc cgcactgtaa
     9601 cattgacccg tgaacgtatt cgtctcgaag accgcgcggt taaaatgtcg gtgaagaccg
     9661 tcggtaaaga gaaagtcggc gtgctggata ttcctggctt ctatgtgggt ttgacagacg
     9721 atgtcaaagt gcaactgcag aaactggaaa aacagaatgt cagcagtgtg atcatcgatc
     9781 tgcgtagcaa tggtggtggg gcgctgaccg aagcggtatc gctctcaggt ctgtttattc
     9841 ctgcgggtcc tattgttcag gtccgtgata acaacggtaa agttcgtgaa gacagcgata
     9901 ccgacggaca ggttttctat aaaggcccgc tggtggtact ggttgaccgc ttcagtgctt
     9961 cggcttcaga aatctttgcc gcggcaatgc aggattacgg tcgtgcactg gttgtgggtg
    10021 aaccgacgtt tggtaaaggc accgttcagc aataccgttc attgaaccgt atttacgatc
    10081 agatgttacg tcctgaatgg ccagcgctgg gttctgtgca gtacacgatc cagaaattct
    10141 atcgcgttaa cggcggcagt acgcaacgta aaggcgtaac gccagacatc atcatgccga
    10201 cgggtaatga agaaacggaa acgggtgaga aattcgaaga taacgcgctg ccgtgggata
    10261 gcattgatgc cgcgacttat gtgaaatcag gcgatttaac ggcctttgaa ccggagctac
    10321 tgaaggaaca taatgcgcgt atcgcgaaag atcctgagtt ccagaacatc atgaaggata
    10381 tcgcgcgctt caacgctatg aaggacaagc gcaatatcgt ttctctgaat tacgctgtgc
    10441 gtgagaaaga gaataatgaa gatgatgcga cgcgtttggc gcgtttgaac gaacgcttta
    10501 aacgcgaagg taaaccggag ttgaagaaac tggatgatct accgaaagat taccaggagc
    10561 cggatcctta tctggatgag acggtgaata tcgcactcga tctggcgaag cttgaaaaag
    10621 ccagacccgc ggaacaaccc gcacccgtca agtaatatca atcaggcaca agaaattgtg
    10681 cctgattttt taacagcggc aagatgccgt aaatcagatg ctacaaaatg taaagttgtg
    10741 tctttctggt gacttacgca ctatccagac ttgaaaatag tcgcgtaacc catacgatgt
    10801 gggtatcgca tattgcgttt tgttaaactg aggtaaaaag aaaattatga tgcgaatcgc
    10861 gctcttcctg ctaacgaacc tggccgtaat ggtcgttttc gggctggtac tgagcctgac
    10921 agggatacag tcgagcagcg ttcaggggct gatgatcatg gccttgctgt tcggttttgg
    10981 tggttccttc gtttcgcttc tgatgtccaa atggatggca ttacgatctg ttggcgggga
    11041 agtgatcgag caaccgcgta acgaaaggga acgttggctg gtcaatactg tagcaaccca
    11101 ggctcgtcag gcggggatcg ctatgccgca agtggctatc taccatgcgc cggacatcaa
    11161 cgcttttgca accggtgcgc gccgtgatgc ctctctggtt gctgtcagca ccggtttgct
    11221 gcagaacatg agcccggatg aagccgaggc ggtaattgct cacgaaatca gccacatcgc
    11281 caatggtgat atggtcacca tgacgctgat tcagggcgtg gtgaacacct tcgttatctt
    11341 tatttcccgt atcctggcgc agcttgccgc gggttttatg ggcggaaatc gtgatgaagg
    11401 tgaagagagc aacggcaacc cgctgatcta ctttgcggtt gcaacggttc tggaactggt
    11461 gtttggtatt ctggcgagca ttatcaccat gtggttctcg cgtcatcgtg aattccatgc
    11521 tgatgccggt tcggcaaaac tggttggtcg cgagaaaatg attgccgcgc tgcagcgcct
    11581 gaaaaccagc tatgaaccgc aagaagcaac cagcatgatg gctttctgca ttaacggtaa
    11641 gtcgaaatcg ctcagtgagt tgtttatgac tcacccgccg ctggataaac gtattgaagc
    11701 tctgcgtacg ggtgaatacc tgaagtaata tgaagtaaat actaaaaagc gcgtcctgct
    11761 cgacgcgctt tttttatgcc ctggatcttg gctgagtgat acgtaaccca ctgatacagg
    11821 cagcaatcac tgccagaata gccgcagcca tcagcgagac gtgtgtacca ttatctccaa
    11881 actgatttag catcagcgcc accagcgccg cgccgctact ctgacccagt agacgagccg
    11941 ttcctaacat gccactggct ccaccgctac gttcgcgagg cgcggaggta ataatggtgt
    12001 ggttattggg tgactggaac aagccaaatc cagcgccaca taagatcatc ggccagataa
    12061 tattgatatc cgcaggtgac gcgggcagca gaaccaggga aaaaagcccc gcagccatga
    12121 tgaataatcc taaagcaccc agcaatcctg catgtacgcg ttcaatcaaa tagcctgcca
    12181 acggagccat caccatcgtt gctaacggcc acggtgtcag aagtaaacct gtttcgactt
    12241 cactacgccc gagcacggtt tgcaggtaaa agggcaggga aaccattgcc agcatttgtg
    12301 cgcagaaaga gcaaacagat gtgcaaatag aaagtgaaaa cagcgggata cgcagtaaat
    12361 ccaccggtag cagcggtact ggaagagaaa gctggcggcg aataaagaaa ataccaacga
    12421 caaccattac aaccagttcc gcagcaatta acgtcagcga ttgcccctga gcgaaaccac
    12481 tcaacgcagt gataagcagg ccgaaggtta acgcgttcat cacggcgctg ggcaggtcga
    12541 aacggggttt actggcgcga gaaccattgg gtggcagaaa acgcatcgcc agaagcaggg
    12601 cgataatacc caacggtacg ttgattaaaa ataaccattt ccaggatgcg atggagagga
    12661 ttgctgcagc aattgtcggc ccggcagcag aagagacggc aacaataaac gagtttatgc
    12721 ccatccctct acccagaaaa cgttgtggat agatcaggcg gataagggcg gtattaacgc
    12781 tcatcaacgc tgcaccgccg aaaccttgta tgacacgcgc aagggtgagt atttgcagcg
    12841 aatcagaaag ggcgcagaac agtgaagaca acagaaaaac gaccagaccg catttataaa
    12901 tacgtcgata gccaaacata tcacccagaa acgaaaacga gagcagggag atgacaatgg
    12961 cgatttgata ggcgttcact acccagatgg aactggctgg cgtggcatga aggtccgtgg
    13021 cgattgttgg cagggcgacg ttggcgattg cgccgtcaag gacagccatc gaaataccaa
    13081 tcacaatggt taatatcgca ccgtatcgct ggggcaatgg caggccgtcg gcctgaactt
    13141 ttggcatagg aattttaaat ctttggtgaa taatcaacag gggtatgatt ctagcactga
    13201 tattcacggt ggatgtcgca gaattgtaac taaccggcaa aagatggatt gcgaccaccc
    13261 tggtgcgaaa ttataataaa aaacagttct gatttttata aaacactcgc aatgaggtga
    13321 taaatggcta acgcagatct ggataaacag cctgattctg tatcttccgt gctaaaagtt
    13381 tttggcattt tgcaggcgct gggtgaagag cgcgaaatag ggataaccga gctgtcgcag
    13441 cgcgtcatga tgtcaaaaag caccgtttat cgctttttac agaccatgaa aaccttaggt
    13501 tatgtggcgc aggaagggga gtcggagaaa tattccctga ccctgaaatt gtttgaactg
    13561 ggcgctcgcg cgttacaaaa cgtcgattta attcgtagcg cagatatcca gatgcgtgag
    13621 atctcccgcc tgaccaaaga aactatccac ctcggcgcac tggacgaaga cagtattgtt
    13681 tacattcaca aaattgactc tatgtacaat ttgcgcatgt attcacggat tgggcgtcgt
    13741 aatccactgt acagcaccgc gattggtaag gtactgctgg catggcgcga tcgcgatgaa
    13801 gtgaagcaaa ttcttgaggg cgtggagtat aaacgcagta ccgagcggac catcaccagt
    13861 acagaagcgt tattaccggt tctggaccag gtgcgcgagc aggggtatgg cgaagataac
    13921 gaagagcagg aagaagggct gcgatgcatt gcggttccgg tatttgatcg ctttggcgtg
    13981 gtcattgccg gtttgagcat ctccttcccg acgttgcgtt tctctgaaga gcgtttacag
    14041 gaatatgtcg caatgttgca taccgcagcg cgcaaaattt ctgcccaaat gggttatcac
    14101 gactatccgt tctgatgaga gtaagaacct gtcggaatat caaacagaca ggttctttat
    14161 ttagcatgag aaaaataaag ttgaaggtgg cgttagatta aacgcagttg atataagaat
    14221 attttactca tgagtgagaa tctggttatt tattacactt aaccattatc gaccacgata
    14281 ttgcttttgc gtaacagcgg gcaatctgtt atccccaaaa aaccactttt agtgtgcaag
    14341 tattgtaccg tgctggtgcc tctggcagtc agataggtac attgcaaacc taattctgcg
    14401 gcattctctt tgcttccaat caaaacgcca tatccgctga gtaataatcc tatccatacc
    14461 agtgctatca gcataactgt gcgaatgatg aatcgcatta caacctcttc tcttttttat
    14521 gttcgcttaa tcgtagcggc aatatgcgct gaagcaagcg actcattccg aaaaagcacg
    14581 aatatcgaca tagttagacg ctgtttaact aacgcatggt agtttaatga cataaggtag
    14641 gtgaaacgga gattggagtg aaaaagtttc gatgggtcgt tttggttgtc gtggtgttgg
    14701 cttgcttgct gctttgggcg caggtattca acatgatgtg cgatcaggat gtacaatttt
    14761 tcagcggaat ttgtgccatt aaccagttta tcccgtggtg atatcatttt ttcagcacta
    14821 gattttcttc ctctccagca gtggtagaat agcgcctcta ctcagaggtg gtgaaatgaa
    14881 cgaagttgta aattcaggcg tgatgaacat tgcgtctttg gttgtatcgg tggcggttct
    14941 tcttatcggg ctcatcttgt ggttttttat taatcgtgcc agttctcgga ctaacgaaca
    15001 gattgaactg cttgaggcgt tgctggatca gcaaaaacgt caaaatgcac tgttacgtcg
    15061 tttgtgcgaa gcaaacgaac cagagaaagc agataaaaag actattgaga gtcaaaaatc
    15121 ggttgaagac gaagatatta ttcgcctggt cgccgaacga taatccttcc tcaatcttga
    15181 gtaaccccgt cgttgaaggg ttactcaatt catcctgatt atacgcactg tcatggtgca
    15241 actgcttcaa attaaaactc tggtctgact tcaaataatt atcatattat ttatttgcgt
    15301 aacgggatat c
//
LOCUS       DAZFDC010000113        15117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFDC010000113 DAZFDC010000000
VERSION     DAZFDC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.113"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(469..1560)
                     /gene="ychF"
                     /locus_tag="ACHJ48_003642"
     CDS             complement(469..1560)
                     /gene="ychF"
                     /locus_tag="ACHJ48_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO4709119.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1677..2261)
                     /gene="pth"
                     /locus_tag="ACHJ48_003643"
     CDS             complement(1677..2261)
                     /gene="pth"
                     /locus_tag="ACHJ48_003643"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO4709120.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2539..2817
                     /gene="ychH"
                     /locus_tag="ACHJ48_003644"
     CDS             2539..2817
                     /gene="ychH"
                     /locus_tag="ACHJ48_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO4709121.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2872..4551)
                     /gene="dauA"
                     /locus_tag="ACHJ48_003645"
     CDS             complement(2872..4551)
                     /gene="dauA"
                     /locus_tag="ACHJ48_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO4709122.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4676..5623)
                     /gene="prs"
                     /locus_tag="ACHJ48_003646"
     CDS             complement(4676..5623)
                     /gene="prs"
                     /locus_tag="ACHJ48_003646"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO4709123.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5774..6625)
                     /gene="ispE"
                     /locus_tag="ACHJ48_003647"
     CDS             complement(5774..6625)
                     /gene="ispE"
                     /locus_tag="ACHJ48_003647"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO4709124.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6625..7248)
                     /gene="lolB"
                     /locus_tag="ACHJ48_003648"
     CDS             complement(6625..7248)
                     /gene="lolB"
                     /locus_tag="ACHJ48_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO4709125.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7462..8718
                     /gene="hemA"
                     /locus_tag="ACHJ48_003649"
     CDS             7462..8718
                     /gene="hemA"
                     /locus_tag="ACHJ48_003649"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO4709126.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8760..9842
                     /gene="prfA"
                     /locus_tag="ACHJ48_003650"
     CDS             8760..9842
                     /gene="prfA"
                     /locus_tag="ACHJ48_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO4709127.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9842..10675
                     /gene="prmC"
                     /locus_tag="ACHJ48_003651"
     CDS             9842..10675
                     /gene="prmC"
                     /locus_tag="ACHJ48_003651"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO4709128.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10672..11064
                     /gene="sirB2"
                     /locus_tag="ACHJ48_003652"
     CDS             10672..11064
                     /gene="sirB2"
                     /locus_tag="ACHJ48_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO4709129.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIASPLTLVVLYIIIKLATTKVPLLG"
     gene            11068..11877
                     /gene="sirB1"
                     /locus_tag="ACHJ48_003653"
     CDS             11068..11877
                     /gene="sirB1"
                     /locus_tag="ACHJ48_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO4709130.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11913..12767
                     /gene="kdsA"
                     /locus_tag="ACHJ48_003654"
     CDS             11913..12767
                     /gene="kdsA"
                     /locus_tag="ACHJ48_003654"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO4709131.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLRQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12915..13022)
                     /gene="ldrA"
                     /locus_tag="ACHJ48_003655"
     CDS             complement(12915..13022)
                     /gene="ldrA"
                     /locus_tag="ACHJ48_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4709132.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13451..13558)
                     /locus_tag="ACHJ48_003656"
     CDS             complement(13451..13558)
                     /locus_tag="ACHJ48_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO4709133.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13957..15057)
                     /gene="chaA"
                     /locus_tag="ACHJ48_003657"
     CDS             complement(13957..15057)
                     /gene="chaA"
                     /locus_tag="ACHJ48_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO4709134.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         3809 a         3810 c         3882 g         3616 t
ORIGIN      
        1 atttttgcat tactaataag aaaaagcaaa aaacatcgat attcattttt ggtcaatggg
       61 ttttctggtc tatcgttgtt attgtgctac tggttatcat tttcatcttg ccaggatcgc
      121 acgtaatcac gttattcggg gactttatct gcgggatatt acccttcagg taagtccttt
      181 tctcgccagc atattggagc gtggtcaata ttgatcggct gtgaaggggg ctgatgtgta
      241 gtggaacaaa aactgacgtt atatcactgc taatgagata tccgtcatct gaacactgat
      301 gtctttaatt attctcgtgg ttcactgtag gcaataagca caaaagtgta ggatgttaca
      361 agaatgatta ggactcggtg aaatgaaaaa tccacgcaat tgcgtggatt ttatatactt
      421 ttgcgctctt catgagattt agcgaaacct catgagacaa taaattaatt agacgttgaa
      481 aaggaagttc atcacatcgc catctttcac gatgtaatct ttaccttctg cacgcatttt
      541 gcctgcttct ttcgcgcctt gttcaccttt gtaagtgatg aaatcttcaa acgagatggt
      601 ttgtgcacgg ataaagcctt tttcaaaatc agtatggatt ttgcccgctg cctgcggcgc
      661 ggttgctcca accggaatgg tccatgcacg cacttctttc accccagcgg tgaagtaagt
      721 ttgcaggttc agcagcttat aaccggcacg gatcacacgg ttcagacccg gctcttccag
      781 cccaagctcc tgcataaact cgtcacgttc ttcgtcgtcc agttcggcaa tgtctgcttc
      841 aacagcagca caaaccggaa ccacaacaga accttctttc gccgcgattt cacgcacctg
      901 gtcaagatat gggttgtttt caaaaccgtc ttcgttgacg ttggcgatgt acattgttgg
      961 tttcagcgtc aggaagctca ggtaacgaat agccgctttc tcttcagcgc ttaaatccag
     1021 cgcgcgcagc atacctgcgt tttccagctg gggcaggcat ttttccagga ccgccagctc
     1081 agctttcgcg tctttatcgc cacctttggc tttcttctgt acgcgatgaa tcgcacgttc
     1141 gcaggtgtcg aggtctgcca gcgccagttc ggtgttgata acttcaatat cgtcagccgg
     1201 gttaactttg ccggaaacgt gaatgatgtt gtcattttca aagcagcgaa caacgtgacc
     1261 gatcgcttcg gtttcacgga tgttggtcag gaactggtta cccagacctt cgcctttcga
     1321 tgcgcctttt accagaccgg cgatatcgac aaattccatg gtcgtgggaa gcgtacgttg
     1381 cggttttacg atttcagcca gttgatccag gcgaggatca ggcattggta cgacgcctgt
     1441 gttcggctca atggtgcaga atggaaagtt ggccgcttca ataccggctt tggtcagcgc
     1501 gttgaacagg gtagatttcc cgacgttggg caaaccgacg ataccgcatt tgaatcccat
     1561 gatttaaatc accttaatat cttaataatc aacctgttat tgctaacaga ttgcagaaat
     1621 ggaaataact ttgcctatta tacacggcac tcggcaaaaa tgccgcagac aacgacttat
     1681 tgcgctttaa aggcgtgcaa tcggttcgtt gctttggtca agccatctgt aaaccacatt
     1741 tcagtacaac gcgccgcttc gtcgatggct tcatcaatta acttctgttc actaacaggc
     1801 ggtttgccta acacaaaacc aacaactttg tttttatcgc ccggatgacc gattccgatg
     1861 cgtaaacggt gaaagttagg gttattaccc aatttactga tgatgtcttt cagtccattg
     1921 tgaccaccat ggccaccgcc caacttaaat ttggcgacgc caggaggcag atccagttcg
     1981 tcgtgggcca ccagaatttc gtccggatta atgcggaaaa aactggccat cgccgcaacg
     2041 gctttgccgc tgagattcat aaatgtagtc gggactaaca ggcggacatc ttcgcctcca
     2101 agggtgactc gcgaagtata accaaagaat ttagcctctt cgcgcagcgg agcgcgcaaa
     2161 cgctctgcca gtaagtcaac gaaccaggca ccagcattat gtcgcgttgc ggcgtattca
     2221 gcaccggggt tcgccaggcc gacaatcaat ttaatcgtca cgtttttttg tcctgagtgt
     2281 gtacataact ggcgcgtagt ttactggttg cggccccgct tgacaaaaaa ctgcgtatca
     2341 aatgcagata acgtaataat tgcctgagtg gactattaga aagtcaaggt gttcaggcgt
     2401 ttatttgtaa agttttgttg aaataagggt tgtaattgtg atcacgcccg cacataaccc
     2461 acagggtgtt gtctatactt tacacataag gaagaggggt attccctgtt acaacccaga
     2521 aagttccgga ggtgacatat gaaacgcaaa aacgcttcgt tactcggtaa cgtgctcatg
     2581 gggttgggtc tggtggtaat ggtggtcggc gtggggtatt caatcctcaa ccagttacca
     2641 cagtttaata tgccccagta tttcgcacat ggtgcagtgc taagtatttt cgtcggtgcc
     2701 attctctggc tggcgggtgc ccgtgttggc gggcatgaac aggtgtgcga ccgttactgg
     2761 tgggttcgcc actatgacaa acgttgccgc cgtagcgata atcgccgtca tagctaacaa
     2821 ataatgcagt ttgctgacca gtcagtttgc gctgactggt caatctcgta cttataaatc
     2881 cgccatcgcc gcgcgacgat tcgggaagaa cgcgaggcgt cccgggatcg gttgaatgcc
     2941 tgcgcgagcc atagtgcgca gtggctggaa ttccacgttg cacacgcgca gttcacatcc
     3001 ttcgggcaga cgcttcacaa aacgctggaa cgcatcaaga ccaccagcat caagtaccgg
     3061 aacggcatcc cacttcagaa tcacaatccg tttgccttca agacgtgact ccaggtccgt
     3121 gaataagcct tcagcagcag caaaaaacag cgggccaata acgcgcagaa ccaggacatc
     3181 gtctggaaca tctacgacta ccggtgccag gcgagtcata cgtgcgatac gacgcataaa
     3241 cagcagcgat gccagcacga tccccacgct gatggcaata accatatcaa acagcacggt
     3301 cagcgacatg cacagcagca tgacaatgat gtcatctttc ggtgcatgac gcagcaagtc
     3361 gaccacttta tgcgcttcac tcatgttcca cgccaccatc aacagcaggg ctgccatagc
     3421 ggaaagcggc agccaggaga gcagcggtgc cagtaccagc agggcaagaa taaccagaat
     3481 agagtggatc accgccgaga taggggaagt tgccccggca cggacgttag cggcagaacg
     3541 cgcgatggca gcggtagcgg taataccacc aaagaacgga gcgatgatat tccccagccc
     3601 ctgtccaacc agttcgctat tcgccttgtg cttcgtcccg gtcataccat ccagcaccac
     3661 ggcgcagagc agagattcga ttgcgccgag cattgccatt gagaatgccg caggcagcag
     3721 tgtgcgaata gaatcccagg ttagcgtgaa ttctgaatta ggcagatccc acggcagcac
     3781 cagttgcggt agcagttgcg gaataccgtt accctgagaa ccatcggcca ggacgtagtg
     3841 gaattgcgaa ccgatggtag caacatgtcc gccgagcagg ttaacaatcc ccatcaccgc
     3901 gcaaccagcc agcaaggccg gaaggtgacc gggtaaacga atgcccagac gcggccagaa
     3961 aacaagaata cctagcgtca caatgccaat ggcagcatca cccacattaa tggtcggcag
     4021 cgccataaat aatgcgccga ctttttgtag ataatgttcc gggacatggg ccatttgcag
     4081 accgagaaaa tctttaatct gcatggtacc gatggtgatc ccgatacccg aggtgaaacc
     4141 taaggtgacg gaaaccggaa tatactcaat caggcgacca aagcgtgcca gacccatcag
     4201 aatcaaaaag atccccgaca gcaaggtcgc aaccagcagt cctgccagcc caaactgttg
     4261 cgaaacggga tagagaatta ccacaaatgc cgcagtcgga ccggaaacgc taaagcgtga
     4321 cccacccgtc agagcaatga caatccccgc aacagctgcg gtatataaac cgtactgggg
     4381 tgccacacca ctaccaatag ccaacgccat cgccagcggg atagcaataa tcccgacggt
     4441 tatcccggca atcaggtcac gggtaaaccg tgcggcagta tatttttctt tccagcaagc
     4501 gtcgatcaga gcgcggaaag gcatcacatg tgaggaaaat attttgttca caataatgtt
     4561 tcatccgtga gcgcatcatc tgtcaactaa atggcaggtg aaggaggcat aggtcataca
     4621 aatggatatt acagacaaaa aaacccgccg cagcgggtct ttgagccggg ttcgattagt
     4681 gttcgaacat ggcagagatc gattcttcgt tgctgatacg acgaatcgct tcggccagca
     4741 tacctgacag ggtcagagta cgcacgttcg gcagtgattt gatttcatcg ctcagcggaa
     4801 tggtatcgca gacaacgact tcatcaatta cagagttacg caggttgttc gccgcgttgc
     4861 cagagaagat cgggtgagtc gcgtacgcaa atacacgttt agcaccacgt tctttcagag
     4921 cttcagcagc tttacacagc gtaccgccag tgtcgatcat atcatcgacc agtacgcagt
     4981 cacgacctgc aacgtcaccg atgatatgca tcacctggga aacgttcgca cgcggacgac
     5041 gtttgtcgat gattgccata tcggtatcgt tcagcagctt agcgatagcg cgggcacgca
     5101 caacgccgcc gatgtccgga gaaaccacaa ttgggttatc cagattcagc tgcagcatgt
     5161 cttccagcag gatcgggcta ccaaatacgt tatcaaccgg aacgtcgaag aaaccctgaa
     5221 tctgttcagc gtgcagatcc actgtcagca cacggtcaac accgacgctg gagaggaagt
     5281 ctgcaaccac tttcgcagtg attggtacac gagcggaacg gacgcgacgg tcctggcgcg
     5341 catagccaaa gtaggggata acagcggtga tacgacctgc ggaagcacga cgcagggcat
     5401 caaccataac gactaattcc atcaggttgt cgttagtagg ggcacaagtg gactggatga
     5461 tgaaaatatc accaccgcgt acattttcat taatttgtac gctgacttcg ccatcgctaa
     5521 agcgacctac agcggcgtcg ccgagtgaag tgtacaggcg gttggcaata cgttgtgcta
     5581 gttccggggt ggcgttacca gcaaaaagct tcatatcagg cacgagaaga acctcaggca
     5641 tgcgtccatt ggtggaaaga atctgccgaa aactgtgcgg gccaggcatg atcctttcca
     5701 ggcggtgtat taaagagcgc gatgcaacgt ctggaacaag gtgacgttgt caccgaaact
     5761 cagcttgccc ggcttaaagc atggctctgt gcaatgggga aagattagcg cctttcgcca
     5821 caaagccatt gagccattcc ggggcttgct ctagcacctg gcgggcttca gactctgtat
     5881 caaattcagc aaagacacag gcccctgtcc cagtcaggcg cgacggggcg tattctaaca
     5941 gccaggaaag caccgcatca acctcgcgaa aacgttttct tgcgataacc tcgcaatcat
     6001 tgctgaattc acattttagc aacgtttcta ttgacctttt tggcgtattg cgcgggagtt
     6061 caggatcttt aaaaatcacc ggagtcggaa tacttacacc agggtgcgcc accagatacc
     6121 acttctctgg cggatccacc ggggttagta tttcaccaac gccttcggca aacgcggcat
     6181 gcccccgaac aaagacagga acatctgcgc ccagcgtcag ccccatttcc gccagctcat
     6241 ccatgcttag cccgcattgc cagagatgat ttaatgccac caggaccgtc gcggcattgg
     6301 atgaaccacc gccgagaccg ccgcccatcg gcaaacgctt gtcaatgctg atattcgcac
     6361 cgcttcccgt cggaagacgc ccgctgtctg ccgcagtttt catcaacaac cgcgctgcgc
     6421 gaacgatcag gttatcttca tgttccacgc cttcaacggg cgttaacaga cggatatccc
     6481 catcgtcacg aagctcaatg ctgatggtgt cgccgtaatc aagaaactga aacagcgttt
     6541 gcagcgtgtg gtaaccatcc gcacgctgac cggtaatgta taaaaacaga ttaagttttg
     6601 ccggagaggg ccactgtgtc cgcattattt cactatccag ttatccattt ttaacttgat
     6661 gcgttgacca ccgtcggtga gttccatatt ggctggcatc gcaggttgcg ttttggtgtc
     6721 ataaccacca taaacaacct tccagttttt gccattctgg ctgtaggtaa tttcgctcag
     6781 gcggtactgg tcgtccagtt tgtagtcggt tgcatcaccc ggtaaaccta aaatccactg
     6841 gcgcaagctg ttgagcggaa ttggcattcc agtcaatttg ccaatcatct cttcggcgtc
     6901 atcggcggta taacgctgac ctttattgtc gactaactgc acgttacccg gttgagcatt
     6961 cagctccagt tccgtgctgc ccaatgggtt agtgagcagc agacggtagc gatcctggcc
     7021 ggtttgctgc cagaaaaagc gggcgtacac tttttgttgg tcagaaatat aagcgaacgc
     7081 gccgcgagtc tgatactgat taagattgcg cacgtcttgc tgatgctgac gccattgtgg
     7141 cgaatccggg cttttgccag gacctttggg cgtggtaacg gaacaggcag tgagcacaag
     7201 agcagccagc ggtagcaggc ggataagacg aaaatcgggc aggggcatag tgatgacaag
     7261 tccttgagat acgttgcagt tataaccctt aatgctagcg tcaccattag tcatcgtcta
     7321 tgttcaagtt gtcttaattg tagaaatcta acgggttcgg gcaattactc caaaaggggg
     7381 cgctcccttt tattgatctc gcgcatacgg tatgatgcgc cgagactaac cttatcaacg
     7441 ttggtattat ttcccgcaga catgaccctt ttagcgctcg gtattaacca taaaacggca
     7501 cctgtatcgc tgcgagaacg tgtatcgttt tcgccggaca aacttgatca ggcgctggac
     7561 agtctgctcg cacagccgat ggtgcagggc ggggtggtgc tgtcgacgtg taaccgcact
     7621 gagctttatc ttagcgtcga agagcaggac aacttgcaag aggcgttaat ccgctggctt
     7681 tgcgattatc acaatcttaa tgaagaagat ctgcgtaaaa gcctctactg gcatcaggat
     7741 aacgacgcgg ttagccattt aatgcgtgtt gccagcggcc tggattcact ggttcttggg
     7801 gagccgcaga tcctcggtca ggttaaaaaa gcgtttgccg attcgcaaaa aggtcatatg
     7861 aaggccagcg aactggaacg catgttccag aaatctttct ctgtagcgaa acgcgtacgc
     7921 actgaaacag atatcggtgc cagcgctgtg tctgtcgctt ttgcggcttg tacgctggcg
     7981 cggcagatct ttgaatcgct ctctacggtc acagtgttgc tggtaggcgc gggcgaaacc
     8041 atcgagctgg tagcgcgtca tctgcgcgaa cataaagtac agaagatgat tatcgccaac
     8101 cgcactcgcg aacgtgccca aattctggca gatgaagtcg gcgcggaagt gattgccctg
     8161 agtgatatcg acgaacgtct gcgcgaagcc gatatcatca tcagttccac cgccagcccg
     8221 ttaccgatta tcgggaaagg catggtggag cgcgcattaa aaagccgtcg caaccaacca
     8281 atgctgttgg tggatattgc cgttccgcgc gatgttgagc cggaagttgg caaactggcg
     8341 aatgcttatc tttatagcgt tgatgatctg caaagcatca tttcgcacaa cctggcgcag
     8401 cgtaaagccg cagcggttga ggcggaaact attgtcgctc aggaaaccag cgaatttatg
     8461 gcgtggctgc gagcacaaag cgccagcgaa accattcgcg agtatcgcag ccaggcagag
     8521 caagttcgcg atgagttaac cgccaaagcg ttagcggccc ttgagcaggg cggcgacgcg
     8581 caagccatta tgcaggatct ggcatggaaa ctgactaacc gcttgatcca tgcgccaacg
     8641 aaatcacttc aacaggccgc ccgtgacggg gataacgaac gcctgaatat tctgcgcgac
     8701 agcctcgggc tggagtagca gtacatcatt ttcttttttt acagggtgca tttacgccta
     8761 tgaagccttc tatcgttgcc aaactggaag ccctgcatga acgccatgaa gaagttcagg
     8821 cgttgctggg tgacgcgcaa actatcgccg accaggaacg ttttcgcgca ttatcacgcg
     8881 aatatgcgca gttaagtgat gtttcgcgct gttttaccga ctggcaacag gttcaggaag
     8941 atatcgaaac cgcacagatg atgctcgatg atcctgaaat gcgtgagatg gcgcaggatg
     9001 aactgcgcga agctaaagaa aaaagcgagc aactggaaca gcaattacag gttctgttac
     9061 tgccaaaaga tcctgatgac gaacgtaacg ccttcctcga agtccgagcc ggaaccggcg
     9121 gcgacgaagc agcgctgttc gcaggcgatc tgttccgtat gtacagccgt tacgccgaag
     9181 cccgccgctg gcgggtagaa atcatgagcg ccagcgaggg tgaacatggt ggttataaag
     9241 agatcatcgc caaaattagc ggtgatggtg tgtatggtcg tctgaaattt gaatctggcg
     9301 gtcatcgcgt gcagcgtgtt cctgctacgg aatcgcaggg tcgtattcat acttctgctt
     9361 gtaccgttgc ggtaatgcca gaactgcctg acgcagaact gccggacatc aacccagcag
     9421 atttacgcat tgatactttc cgctcgtcag gggcgggtgg tcagcacgtt aacaccaccg
     9481 attcggcaat tcgtattact cacttgccga ccgggattgt tgttgaatgt caggacgaac
     9541 gttcacaaca taaaaacaaa gctaaagcac tttctgtact cggtgctcgc atccacgctg
     9601 ctgaaatggc aaaacggcaa caggccgaag cgtctacccg tcgtaacctg ctggggagtg
     9661 gcgatcgcag cgaccgtaac cgtacttaca acttcccgca ggggcgcgtt accgatcacc
     9721 gcatcaacct gacgctctac cgcctggatg aagtgatgga aggtaagctg gatatgctga
     9781 ttgaaccgat tatccaggaa catcaggccg accaactggc ggcgttgtcc gagcaggaat
     9841 aatggaatat caacactggt tacgtgaagc aataagccaa cttcaggcga gcgaaagccc
     9901 gcggcgtgat gctgaaatcc tgctggagca tgttaccggc agagggcgta cttttattct
     9961 cgcctttggt gaaacgcagc tgactgacga acaatgtcag caacttgatg cgctactgac
    10021 acgtcgtcgc gatggtgaac ccattgctca tttaaccggg gtgcgagaat tctggtcgtt
    10081 gccgttattt gtttcgccag cgaccttaat tccgcgcccg gatacggagt gtctggtgga
    10141 gcaggcactg gcgcggttgc ctgaacaacc ttgccgtatt ctcgatctcg ggacgggtac
    10201 cggggcgatt gcgcttgcgc tggctcgcga gcgcccggac tgcgaaatta ccgctgtaga
    10261 tcgtatgccc gatgctgtcg ccctggcaca acgtaatgcc cagcatctgg cgatcaaaaa
    10321 tatccacatt ctgcaaagcg actggtttag cgcgctagcc gggcagcagt ttgcgatgat
    10381 tgtcagcaat ccgccgtata ttgacgagca ggaccctcat cttcaacaag gcgatgtccg
    10441 ctttgagccg ctcactgcgc tggttgcggc agacagtgga atggcagaca tcgtgcatat
    10501 catcgaacag tcgcgtaacg cgctggtatc cggcggcttt ctgcttctgg aacatggctg
    10561 gcagcagggc gaagcggtgc gacaggcatt tatcctcgcg gggtatcatg acgtcgaaac
    10621 ctgccgtgac tatggtgata acgagcgcgt aacgctcggc cgctattatc aatgacaagt
    10681 ttttctacac tgcttagtgt tcatcttatt agtatcgcgc tttctgttgg gctattaacc
    10741 ttacgtttct ggctacgtta tcagaagcat ccacaggcat ttgctcgctg gacgcgcatt
    10801 gtgccgccgg ttgtcgatac ggtgttattg ttaagcggca ttgcgttgat ggctaaagcg
    10861 cacatcctgc cattttccgg gcaggcacag tggctgactg aaaagctgtt tggagttatc
    10921 atttatatcg ttttgggttt tattgcactc gattatcgtc gtatgcacag tcagcaggcg
    10981 cgcattattg cctccccgct gacgttggtg gtgctgtaca tcatcattaa actcgccacc
    11041 acaaaagtac cgttactggg gtaagtcatg agatcgttag ctgatttcga atttaataaa
    11101 gcgccattgt gcgaaggcat gatcctggct tgcgaagcaa tccgccgcga ttttccctcg
    11161 caagatgttt acgacgaact ggagcgtctc gttagtctgg cgaaggaaga aatcagccag
    11221 cttctgcctt tagaagagca gctggaaaaa ctgatcgcgc tgttttacgg cgactgggga
    11281 tttaaagcct cacgcggcgt ttatcgtctt tccgatgcat tatggctgga ccaggtgtta
    11341 aagaatcgac agggcagtgc ggtatcatta ggtgcggttt tattatgggt cgcgaatcgt
    11401 ctcgatttgc cgctgctgcc ggtgattttc cctacgcagc tgatattgcg cattgaatgt
    11461 ccggatggcg aaatttggct gattaatcct tttaacggtg aatcgttaag cgaacatatg
    11521 ctggacgtat ggttaaaggg aaatatcagc ccgtcggcgg aactgtttta tgaagacctt
    11581 gatgaagctg ataacattga ggtaatccgc aaattgctgg atacactcaa agcctcgttg
    11641 atggaagaaa atcagatgga gctggcgtta cgcaccagcg aagctttatt acaattcaac
    11701 ccggaagatc cctatgaaat tcgcgatcgc gggttgattt atgcgcaact ggattgcgaa
    11761 cacgttgcgt tgaacgattt aagttatttc gttgaacagt gtccggaaga cccgatcagc
    11821 gaaatgatcc gtgcgcaaat aaataacatc gcgcataaac atattgtgct gcattaatta
    11881 atcgacattt tactcaaggt taaggcgatc ctatgaaaca aaaagtggtt agcattggcg
    11941 acatcaacgt agcaaatgac ctgccgttcg tactgtttgg cggtatgaac gtgctggaat
    12001 ctcgcgatct ggcgatgcgc atttgcgagc actacgtaac cgtgacccag aaactgggta
    12061 tcccttacgt gttcaaagcc tcttttgaca aagccaaccg ctcctccatc cactcttatc
    12121 gtggaccggg cctggaagaa gggatgaaaa tcttccagga gttgaagcag acttttggcg
    12181 tgaaaattat caccgacgtt cacgaaccaa gtcaggcaca gcccgttgct gatgtcgtgg
    12241 atgtgattca gttgccggcg tttcttgctc gccagactga cctggttgaa gccatggcga
    12301 aaaccggtgc ggtaattaac gtcaagaaac cacagtttgt cagcccggga cagatgggta
    12361 atatcgttga taaattcaaa gaaggcggca acgaaaaagt gattctttgc gatcgtggtg
    12421 ctaacttcgg ctatgacaac ctggttgtcg atatgctggg cttcagcatt atgaagaaag
    12481 tgtctggtaa ctcgccggtg attttcgacg tgacccacgc attgcaatgc cgcgatcctt
    12541 ttggcgcagc ttccggcggt cgtcgtgctc aggtggctga gctggcacga gccggtatgg
    12601 cggtaggtct ggcggggctg tttattgaag cgcatccgga tccggaacat gcgaaatgtg
    12661 atggtccatc cgcgctgccg ctggctaaac tggaaccgtt cctcaggcag atgaaagcga
    12721 ttgatgatct ggtgaaaggt ttcgaagaac tggataccag caagtaatct tttttgcttg
    12781 aaaaataaag tcttagcgtt ctgcgttaag acttttttca tgggtgccgg atacaaaaaa
    12841 ggccgcaggc tgttaccctg cggccggttt cgggcgcata ttgccatcac ggcagcctga
    12901 cgcccgtttt cgccttactt ccggttacgc caccagccga caatcgctgc ggtaataatt
    12961 cccgccagga tcggtgctgc caggtcgtgc cagaaaatca tggcaaactg cgcgagcgtc
    13021 atatagccgc cttgttgtaa tgacaacatt ttgcggctat tcttgaattg ttgatggttc
    13081 aagattagcc cccgttctgt tgtcaggttt tacctctcaa cgtgcggggg ttttctcttt
    13141 ccagcaacca atgccaccag ggataaagcc cccgcaacat tgcgcctcac cggataacgc
    13201 cggcttggtg tggatactac gactcaattc atcttcactt catccctgaa atgtttgcaa
    13261 tgaagagtgc attccggttt ttcaacagct gttacagtcg tttcatgagt gctctggatg
    13321 atgcttccag ctcgggttgc caatatttac ttgtggaagt gataaagaca aaaatggccg
    13381 caggctgtta cccctgcggc cggtttcggg cgcatattgc catcacggca gcctgacgcc
    13441 cgttttcacc ttacttccgg ttacgccacc agccgacaat cgctgcggta ataattcccg
    13501 ccaggatcgg tgccgccagg tcgtgccaga aagtcatggc aaactgcgcg agcgtcatat
    13561 agccgccttg ttgtaatgac aacattttgc ggctattctt gaagtgtctg gtttcaagat
    13621 tagccctgtt gtcaggtttt acctctcaac gtgcgggggt tttctctttc cagcaaccaa
    13681 tgccaccagg gataaaaccc ccgcaacatt gcgcctcacc ggataacgcc ggcttggtgt
    13741 ggatactacg tctcaattca tcttcacttc atccctgaaa tgtttgcaat aaagagtaca
    13801 ttccggcttt tcaacagctg ttgcagtggt ttcgtgcagg ttgtggtaca ggctcgcaat
    13861 tctgattaac gacctgtcca ggtagtatga agaaatacaa taagcaggaa tttatattat
    13921 ctcccccggt ttccgtcatc ggaggagata taactgtcag gcaaatatcg tcatcaaata
    13981 ggcggcaaac agtgccagat gcgctgcgcc attgagcacg ttagtacgtc cggtggagaa
    14041 ggagatatgg cacagcacta aagaggccac catcaccacc atttctggcg caccaagtgc
    14101 aaactgcaat tcgttacccg tcataaaggc aattagcgtg acgacaggta cggtaagcga
    14161 aattgttgct aacactgaac caaagaacag attcatcgcg cgctggacct ggttgttcaa
    14221 cactgctttt aatgcaccta aaccttccgg cgacagaatc aacagtgcca ccaggaagcc
    14281 agtaaaggcg acaggggcat tcatgctgtc gagcaaagtc tccagcgggc tggcgttcat
    14341 tttggtgact gcaataacgg caatcaggtg gataatcaac cagatagcat gccacaggct
    14401 gctatgggca gacggtttac cgtggtgcgg gtcgtcgtca tcactgtcat cttcgtgctc
    14461 gtagacaaac aaactttgat gcgttttggt ctggatcagc aaaaatacac catacattgc
    14521 cgcagaaatt aatgctacca gtaacgcctg accggttgaa aaattcgccg caggcagagc
    14581 cattggaaat accagtacga ttatcgccag ggggaacagc gcaattaaat actgcttaat
    14641 accaaacaga ttcatatatt gggtggcaaa cttacgaccg cccaacaata atgaaaagcc
    14701 gaccagccca ccggtaacaa tcataatgat cgaatagagc gtatcacgca ttagcgttgg
    14761 cgcggcgtcg ccggttgcca ttaaagctga aatcaaacta acttcaagaa taaccactga
    14821 aaggctaaga ataagcgaac cgtaaggttc tcccaggcga tgggctaata cgtccgcatg
    14881 acggacaaca ctaaaggcgc tacttaaaat accaataagc gcaagaagat tgatggcaat
    14941 gaccactggt agtgtctggc tgcttcccca caggaacagc actaccagcg ccagaaccgg
    15001 gaaaataagc gaagtctcct tgtggcgggt ttttaccgcc tcttgagcat ttgacattat
    15061 ggttatccct ttgcagatga atttatcgaa aatgtaaaaa ataggtagga aaaataa
//
LOCUS       DAZFDC010000114        15093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFDC010000114 DAZFDC010000000
VERSION     DAZFDC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.114"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..340
                     /locus_tag="ACHJ48_003658"
     CDS             68..340
                     /locus_tag="ACHJ48_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO4709135.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(341..1213)
                     /gene="rluF"
                     /locus_tag="ACHJ48_003659"
     CDS             complement(341..1213)
                     /gene="rluF"
                     /locus_tag="ACHJ48_003659"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO4709136.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1561..2508
                     /locus_tag="ACHJ48_003660"
     CDS             1561..2508
                     /locus_tag="ACHJ48_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO4709137.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTNISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGEMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2578..3384
                     /locus_tag="ACHJ48_003661"
     CDS             2578..3384
                     /locus_tag="ACHJ48_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4709138.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEIADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3394..3801
                     /locus_tag="ACHJ48_003662"
     CDS             3394..3801
                     /locus_tag="ACHJ48_003662"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO4709139.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQNEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3801..4295
                     /locus_tag="ACHJ48_003663"
     CDS             3801..4295
                     /locus_tag="ACHJ48_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO4709140.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIYKINEQ
                     LIAN"
     gene            4361..5158
                     /locus_tag="ACHJ48_003664"
     CDS             4361..5158
                     /locus_tag="ACHJ48_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO4709141.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLLAPASTALDQLDD"
     gene            5169..5993
                     /locus_tag="ACHJ48_003665"
     CDS             5169..5993
                     /locus_tag="ACHJ48_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO4709142.1"
                     /translation="MEQRKITRGDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPVKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6045..7274
                     /gene="sorE"
                     /locus_tag="ACHJ48_003666"
     CDS             6045..7274
                     /gene="sorE"
                     /locus_tag="ACHJ48_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO4709143.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            7657..8346
                     /gene="pepE"
                     /locus_tag="ACHJ48_003667"
     CDS             7657..8346
                     /gene="pepE"
                     /locus_tag="ACHJ48_003667"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO4709144.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8437..10068)
                     /locus_tag="ACHJ48_003668"
     CDS             complement(8437..10068)
                     /locus_tag="ACHJ48_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO4709145.1"
                     /translation="MLTLLNLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLAMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMSSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10288..13971)
                     /gene="metH"
                     /locus_tag="ACHJ48_003669"
     CDS             complement(10288..13971)
                     /gene="metH"
                     /locus_tag="ACHJ48_003669"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO4709146.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14171..14995
                     /gene="iclR"
                     /locus_tag="ACHJ48_003670"
     CDS             14171..14995
                     /gene="iclR"
                     /locus_tag="ACHJ48_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO4709147.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3559 a         3973 c         3988 g         3573 t
ORIGIN      
        1 tattatcagt tctgctcgca aacgaattcc cgtacactga actctctggc tcactgaggg
       61 caccatcatg gcactccccc gtattaccca aaaagagatg actgaacgcg agcagcgtga
      121 acttaaaaca ctgctggatc gggcgcggat tgcgcatggt cgcgtactga ccaactccga
      181 aaccaacagc attaagaaag agtacatcga taaattaatg gtcgagcgtg aggctgaagc
      241 gaaaaaagcc cgccagttaa agaaaaagca ggcttataaa ccggatcctg aagcgtcatt
      301 tagctggtca gcaaatacgt cgacgcgcgg caggcgctga ttagcgcccc ttcttttttc
      361 gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt tccgccgttt
      421 tttccatctt aacgactgga cgtttgatgc ccgctgtttt cggtttggcc ttcgctttag
      481 gttttgcctc ggaagaggaa ttttcgatga gcttaaagag gtcgattaac tcatcgtcgg
      541 ttaaatcgcg ccactccccc agcggaatgc cgcttaagct gacgttcata atgcgcgtac
      601 gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc tgacggttca
      661 gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact ttgcactttt
      721 tagtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat tcatcggtaa
      781 tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca cggaggatct
      841 tattcaccag atcgccgtga ttggtgagga aaatcagccc ctgggagtct ttatccaggc
      901 ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta tcgcgctcgc
      961 catcttcggt ggtgctgaca atgcctacgg gcttgttcag ggcgataagt accaaatctt
     1021 cggcttcccg aggctcaatc aactgaccgt ttactttcac aacgtcgccg ggtttcacct
     1081 gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg atatagcgat
     1141 ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa cggactgatg
     1201 agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct tagcggggaa
     1261 aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt ttatcacaaa
     1321 agtgtctccg gaagtacaat gcaaatttgc gtggcaaaat aaggataatc aacatattac
     1381 gaatcgctgc acacctgtgc tgataactac tgcgcagaga aacaaatgtg caagagaaag
     1441 cgctatttct ccccatgcaa agagtgaaaa atcgctttga aaggccatca tgatggggtt
     1501 atatcagatc aagcggtgag aaagcgtatt tgcacaaatg tgcagaacca ggaggcggca
     1561 atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta ttacgaacag
     1621 gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt accgcaccaa catcagccgc
     1681 ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta tgactacaac
     1741 gaaaatctct ggctggagca gcaactgaag caaaagtttg gcctgaaaga cgttgtggtg
     1801 gtgtcgggaa atgatgagga tgaagagact caactggcga tgatggggtt acacggcgcg
     1861 caactgctgg atcgcttgct ggagcctggc gatattgtcg gtttttcctg gggtcgcgcg
     1921 gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat cgcggcagtt aatctgcgta
     1981 ccaattattg gcggaccgtc cggtaaactc gaaagccgct atcacgtaaa cacattaacc
     2041 tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg cggattttcc ggctctactg
     2101 gataacccat taattcgtaa tgggatcatg cagtctcagc actttaaaac catctctgcc
     2161 tactgggata atctggatgt cgccctggtg ggaattggct caccggccat tcgcgacggc
     2221 gctaactggc atgcgtttta tggtggtgaa gagagtgacg acctgaatgc ccgccaggtt
     2281 gctggcgata tttgctcgcg cttttttgat attcacggcg aaatggttga aacgaatatg
     2341 agcgaaaaaa cactctctat cgaaatgaat aaattaaagc aggcacgata ttccattggc
     2401 attgccatga gtgaagaaaa atacagcgga attattggtg cactgcgtgg aaaatatatt
     2461 aattgtctgg taacgaatag cagcacagct gaactattac tgaaataaaa atcttcccga
     2521 tgtttatcgt aataaaaatt acggcgggtc cctattatct aaaatcagga gtctgttatg
     2581 caaacgtggt taaatttgca ggataaaatc attatcgtca ccggcggcgc atccggtatt
     2641 ggtctggcga ttgtagagga attattagca caaggcgcaa acgtacagat ggtagatatt
     2701 cacggtggcg atggacaata tgaaggtcat aaaggctatc agttttggcc gaccgatatt
     2761 tccagcgcca aagaggtaaa tcatacggta gcggaaatta tccagcgttt tggtcgcatc
     2821 gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc tgctggtcga tgagaaagcg
     2881 cctgccgggc agtatgaact caacgaagct gcattcgaaa aaatggtcaa tatcaaccag
     2941 aaaggcgttt ttctgatgtc gcaggcggtg gcgcgacaga tggtcaaaca acatgatggc
     3001 gtgattgtga atgtttcctc agaaagtggg ctggaaggct cagaaggcca aagctgttac
     3061 gccgctacca aagccgcgct caatagcttc acgcgctcct ggtcgaaaga gctgggtaaa
     3121 cacggtatcc gtgtggtcgg tatcgcgccg gggattctgg aaaaaaccgg gctacgcacg
     3181 ccggaatatg aagaggcgct ggcgtggacg cgcaatatca ccgtcgagca gctgcgtgaa
     3241 ggctatacca aaaacgccat tcctattggg cgcgccggaa gattagcaga aatcgctgat
     3301 tttgtttgtt atctgctgtc tgaacgcgcc agctatatca ccggagtaac cactaacatt
     3361 gcgggcggca aaacgcgcgg ctaaggaggc tttatggtca acgccatttt ttgtgcccac
     3421 ggcaaattag cttgcgccat gctggaatcg gttcagatgg tttacggcaa cgccaatgtt
     3481 gaagccgttg aatttgtgcc cggtgaaaac gccggagaca tcgtagcaaa gctggaaaaa
     3541 ttagtaagca ttcacaatca aaatgagtgg ctgattgccg tggatttaca gtgcggcagc
     3601 ccgtggaatg cggcagcgat gcttgcgatg cgcaatcctc gtctgcgggt aatcagcgga
     3661 ctttctctgc cgctggcgct cgaactggtt gataaccagg acagcatgaa tgttgatgaa
     3721 ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg ttgtctggaa acaactggca
     3781 acgacagagg aggatttctg atgaacatta cgctcgcccg cattgatgac cgcctgatcc
     3841 acggtcaggt cactaccgtc tggtcgaaag tggctaacgc ccagcgcatt attatctgca
     3901 atgacgaagt ttataacgat gaagttcgcc ggacattatt gcgtcaggct gcgccaccgg
     3961 gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc ggtttatcac aacccgcaat
     4021 atcaggacga aaccgttttt tatcttttca ctcgcccaca ggatgcctta gcgatggttc
     4081 gccagggggt aaaaataggc accctgaata ttggcggcat ggcctggcgg cccggtaaaa
     4141 aacagttaac caaagcggtt tcattagatg atgatgatat taacgcattt catgagttaa
     4201 ataatctagg cgtcatactg gatttacgcg tcgtagcgtc agatccctca atcaatatta
     4261 tctacaaaat aaacgaacag ttaatcgcaa attaaataat agcgcctgtg tacatatgct
     4321 ttaacaggca gggatgacga cacttaaaag gtgacaaatc atggaaataa gtaccctaca
     4381 aatcattgct atatttcttt tttcctgtat tgccggaatg ggcagcgtgc tggatgaatt
     4441 tcagactcat cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg gtgatttaaa
     4501 aaccggaata atactcggtg gtacgctgga attgatagct ctcggctgga tgaacgtcgg
     4561 cgcggcgcaa tctccggatt ctgcactcgc cagcataatc tccgccattc tggttatcgt
     4621 tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg cctgtggctg cggcaggcca
     4681 ggtgctgacg gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg cggataaagc
     4741 agcggaagaa gcacggtttc gcactctcga tattctgcat gtctccgcgc ttggcgtgca
     4801 ggcgctgcgc gtcgccattc cggcactgat tgtctcgctg ttcgtcagcg ccgatatggt
     4861 gagcaatatg ttaaacgcca ttcccgaatt tgtgacccga ggtctgcaaa ttgctggcgg
     4921 ttttatcgtg gtagtcggtt acgccatggt gctacgtatg atgggcgtga aatatttgat
     4981 gcctttcttt ttcctcggtt tcctcgcagg tggctacctc gatctcagtc tgctggcgtt
     5041 cggtggcgtc ggcgtgatca tggccctggt ctacatccag ctaaatccac agtggcgtaa
     5101 agctgaacct caactcctgg cccccgcttc caccgccctt gaccaacttg acgattaacg
     5161 gagccatcat ggaacagaga aaaattacac gcggcgatct ggtgagcatg tttctgcgct
     5221 ccaacctgca acaggcgtcc tttaactttg aacgtattca cgggctgggt ttttgctacg
     5281 acatgatccc cgccatcaag cgactttacc cagtaaaaga ggatcaggtt gcggcgctca
     5341 ggcgacacct ggtgttcttt aataccacgc cagccgtatg tggcccggtc atcggcgtca
     5401 ctgccgccat ggaagaggcg cgggccaacg gcgcggaaat tgatgacggc accattaacg
     5461 gcatcaaagt cggtctgatg ggaccattgg caggggttgg cgatccactg gtctggggaa
     5521 cgctgcgccc gattaccgcc gcgctcggcg catcgctggc gctgtctggc aacattctcg
     5581 gcccgctgct gttcttcttt attttcaacg cggtgcgtct ggcgatgaag tggtatggcc
     5641 tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg aatgtgctgc
     5701 aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg ctggtcacca
     5761 aatggacgtc aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc gatggctcca
     5821 ccgtcaccat gaccgtgcag aacattctcg atcaactttg ccctggtttg ctggcgctcg
     5881 gtctgacgct actgatggtt cgtctgctca acaaaaaaat taacccggta tggctgattt
     5941 tcgccctgtt tggcttaggg attatcggca atgcgctggg cttcctgtcc tgattcttcg
     6001 ccccggcacg actgccgggg ccatcgttca acatgaggtg gtttatgaaa acaacagctc
     6061 tgcgtcttta tggtaaacgt gatttacgcc tggaaacctt tgaccttcct gaaatgcagg
     6121 aggatgaaat cctcgcgacg gtggtcactg acagcctgtg cctctcttcc tggaaagagg
     6181 ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac ccaatcatca
     6241 tcggccacga gttttgcggc gatattctgg ccgtgggtaa aaagtggcag cacaaattcc
     6301 agccgggtca gcgttatgtg attcaggcca acctgcaact ccccgaccgc ccggactgcc
     6361 ccggctactc cttcccgtgg gtaggcggcg aggccacgca tgtggttatt cccaacgagg
     6421 tcatggaaca agattgcctg ctggcatacg acggcgaaac ctattttgaa ggctcgctgg
     6481 ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt caggaaggta
     6541 gttataacca cacgatgggg attcgcccgc aagggcgcat gctgatcctc ggcggcaccg
     6601 gaccaatggg actgttggcg attgattatg cgctacatgg acccgttaac ccgtcgctgc
     6661 tcgtcattac cgataccgac aacgataaat tgagttatgc gcgcaagcac tatccatcag
     6721 aaccgcaaac actgattcat tatctcaatg ccgccgatgc agcatttgat acgctaatgg
     6781 cgctgagtgg cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat gaaggactgg
     6841 tgactctcgc ctcttccttg ctggcgacag atggttgcct gaatttcttc gccggaccgc
     6901 aggataaaca tttcagcgcg ccaattaatt tctacgatgt gcattatgca tttacccact
     6961 acgtgggcac gtcaggcggc aataccgacg acatgcgcgc agcggtcaaa ttgattgaag
     7021 agaaaaaagt gcaggccgca aaagtggtaa cacatattct tgggctgaat gccgcgggcg
     7081 aaaccacgct tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat accgggaaat
     7141 acctgccgct gacgtcactc acgcagattc aggatcaagc actggcggcg attctggcgc
     7201 gtcatcaggg gatctggtcc ggtgaggcgg agcaatatct gctcactcat gcagaggcaa
     7261 tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc tggctggtca ccgcggcaac
     7321 ttcgcgacct gatgtgcggc tgattgctga agcggcccaa tgtgcacgag aaggataaaa
     7381 aaacatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg tttatgccgg
     7441 atgcggaata aagccttatt cggcctacat aatctcgaaa attcaatata ttgcagttat
     7501 ctggtaggcc tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc agtctcagcg
     7561 cccggtgcaa tacgtgacat cgtgagccat gtcagtaagt ggttaaattc gcagtggtag
     7621 catgccttcc gtgatttctt actaaccgga gaagtaatgg aactgctttt attgagtaac
     7681 tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctgattgc tgaacagttg
     7741 cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac ctgggatgat
     7801 tacacagcga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac cggtattcat
     7861 agcgttgtcg atcccgttgc cgcgattgaa aatgcggaaa ttgtgattgt cggcggcggt
     7921 aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggctcc aattactgac
     7981 gtggttaaac gtggcgcgct gtatattggc tggagcgcag gcgctaacct tgcttgccca
     8041 accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga tgcgctaaat
     8101 ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg ccataaaggt
     8161 gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact gacgattatt
     8221 ggtctaccgg aaggtaactg gatcacagtg agtaaaggtc acgcaacgct gggtggcccg
     8281 aataccacct atgtgtttaa ggccggtgaa gaagcggttc cgctggaagc tggtcaccgt
     8341 ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct gatgcgacgc
     8401 ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc gtccctcatc
     8461 ttcatcaggc tgttccagca cactgtaagc caccgaacaa aacagcgagt tcagacgctg
     8521 catatctccc agtaagccta aatgtagcga actggtttca atgctttgca cgttttgctg
     8581 atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa agcgatgctt
     8641 gctgcggcgc aaacgacgag cgctggtgac atcgccagag aagaacaccg acattgccag
     8701 ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaagc catcaagcga
     8761 aaatgctcgc cttgcagcca gcgatttgtc ggcaatttca ctactcatgc gctcgacgat
     8821 atcggaggcc tgttcaaggt tgagcgacat ttcgatgatc tccgcccagc ggcgcgactc
     8881 ttcttctgcc agttcctctt ttggcatccg cgccagatac agcttaatgg cggtatagag
     8941 aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccttggct cgccgtgcat
     9001 cactttattc agcccttcca tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg
     9061 cgcggcgttc gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca gatgtttagg
     9121 ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa accgtgccat
     9181 cgggtcaaca aatggcagca tggccaggca acgtacaagg ttgtagaaga cgtggaaata
     9241 gatcaccagt tccgcttttg gcaatgacaa cttccccatt gtctctgcca gcaaatggac
     9301 aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca gcgcgacacg
     9361 gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg aaccgaggtt
     9421 agcaccaatc accagacaga gcgccacggg gaaggagata atgcctgcgg cggtcagagt
     9481 cgcggtcagc agtacagcag caaggctgga gtagctgata atggcgaaca tcgcgccaat
     9541 cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa cgccgtttgc
     9601 ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagcaaaa tcagcccaag
     9661 accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat
     9721 cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga gaatacgcgc
     9781 cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg cgagggctac
     9841 cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc tctgtaccag
     9901 tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag
     9961 gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa tatgagtccc
    10021 ccagaccagc agggcgacgg cagaaagcag gttaagcagc gttaacacga gttcaggttc
    10081 tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa acacgctgac
    10141 tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt ccagcctgtt
    10201 tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg ccctgtttgt
    10261 tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat tcggtgccag
    10321 ccagcgctca acatcggaga cgctcatacc tttacggcgg gcatagtctt caacctgatc
    10381 gcgctgaatt tgcgccaccg cgtagtactt gctgtccggg tggctgaaat accaacccga
    10441 aaccgatgca ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg tatgtttttc
    10501 cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg ccgggtagcc
    10561 cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc tgaggttctc
    10621 gttcggcgca tagccccagt agactttacg cacacgctca tggagatact ccgcaaaggc
    10681 ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat cgtgctgcgc
    10741 ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa atgcgccgat
    10801 gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt agttagcgaa
    10861 tcccgttttt tcggtctgtt gacgcagatg gtggctgacg ttgatcacat gggtgcgcgt
    10921 ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca ggcccaccac
    10981 gccacgcgga ttcagcgttt tctcggcgct taatttatcc agcatgtcgt tggcgtcttt
    11041 aaacagccgc tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg gatacttccc
    11101 ggccagcgac caggtcataa agaacggtgt ccagtcgatg taattacgca gagtttcgat
    11161 gctggcttca acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt aagcctgcca
    11221 gtcaaaagcg aagtcgttat cgcgcgccgc ttccagcgtg accggtggtg tgcgtggttt
    11281 cttgcgcccg tgctgaatac gtacggtttc gtactccttg cgggtacgag cgacaaaatc
    11341 atcgcgctga gtatcggaaa gcagcgccgc caccacacca acggtgcgcg aggcattctg
    11401 cacatacacc gtcgggccgc tgtagttctg ctcgattttc accgccgtgt gcgcttttga
    11461 ggtcgtcgcg ccaccgatca gtaacggaat ggtgaagccc tgacgctcca tctctttcgc
    11521 cacgttaacc atctcgtcca gcgacggcgt gataagcccc gaaagtccaa tcagatcagc
    11581 attcacttct ttagcggtac ggagaatttt ttccgcaggc accataacgc cgagatcgac
    11641 aatttcgtag ttgttacatt gcagcaccac accaacgata tttttaccga tgtcgtggac
    11701 gtcgcccttc acggtggcga tcaccatctt gccgttggtt ttgccctgct ctttgctggc
    11761 ttcaataaac ggttcgaggt aggccactgc ctgcttcatc acacgcgccg atttgaccac
    11821 ctgtggcagg aacattttcc cttcgccgaa cagatcgccg accacgttca tgccgtccat
    11881 tagcgggcct tcaatcactt caatcgggcg cgtagcctgc tggcgggctt cttcggtatc
    11941 ctgctcgata aactcagtaa tgcctttgac cagcgagtat tccagacgtt tattcacttc
    12001 ccacgagcgc cactccgcct gctgggcgtt agcggtgtcg tcggttttgc tgccgcgata
    12061 tttctcggca agctccagta aacgctcagt gccatcatcg cgacgattaa gaatcacatc
    12121 ttccaccgcg tcgcgcagtt cagcgggcag gtcgtcgtaa atcgccagtt gcccggcgtt
    12181 gacgatcccc atatccatgc cattgcgaat agcgtagtag aggaacaccg cgtgaatggc
    12241 ttcgcgcacc ggatcgttgc cacggaatga gaaagaaacg ttagatacgc cgccggaaat
    12301 cagcgcgtgc ggcagttcgc gtttgatgtc ttcgcaggcg ccgataaagt cctgcgcata
    12361 gttgttgtgc tcttcaatgc cggtggcgac cgcgaagatg ttcgggtcga agatgatatc
    12421 ttctggcggg aagccaacct cttcggtgag gattttgtac gcccgacggc aaatctcaat
    12481 tttccgtgcg cgggtatcgg cctgcccctg ttcgtcaaag gccattacca ccaccgctgc
    12541 accgtaacga cgcaacagtt tcgcatgatg gataaaggcg tcgacgccct ctttcattga
    12601 gatagagtta acaatgcctt tgccctgaat acacttcagc cccttttcga tgacctccca
    12661 ttttgaggag tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg caatcagatt
    12721 gagaaaacgc accatcgccg cttcggcatc gagcatccct tcatccatgt tgatatcgat
    12781 aatctgcgcg ccgttttcca cctgctgacg cgctacatcc agcgcctcgc tgtatttctc
    12841 ttctttgatc aggcgcttga acttagcgga accggtgacg ttggtgcgtt cacccacgtt
    12901 cacaaacagg ctatcgtcgc caatgttcag cggctccagg ccggacaaac ggcaggctac
    12961 gggaatttca ggcagtttgc gcggcgctaa tccttctact gcacgactca tcgctgcaat
    13021 atgttgtggc gtggtgccac agcagccgcc gacgatattg agaaaacccg cttgcgccca
    13081 ttcacgtatc tgttttgcca tcgtgtcggc gtcgagatcg tactcaccaa aggcgttggg
    13141 tagcccggcg ttcgggtgcg cggtgacgta gcattccgca atccgtgata gttcctgcac
    13201 gtactggcgc agttcatctg gccccagcgc gcagttgagg ccaaaagtca gggcttcggc
    13261 atggcgcagg gagttgtaaa atgcttcggt ggtctgtccg gagagcgtgc gcccggaggc
    13321 gtcggtgatg gtgccggaga tcataatcgg cagctcaacg cctaacgctt cgaactccgt
    13381 tttcaccgca aagaccgccg ctttggcgtt aagggtgtcg aaaacggttt caatcagaat
    13441 cagatccgca ccgccttcca ctaatgcttt ggtggattcg cgataggccg ccaccagctg
    13501 gtcaaaagtg atgttgcgga atgccggatc gttcacgtcc ggagaaatgg acgccgtgcg
    13561 gttggttggg ccgagaacgc cggcaacgta gcgcggtttc tccggcgtgc gcgcggtcca
    13621 ctcgtcagca caggcgcgcg ccagtttcgc cgccgcaaag ttgatttccg ccgacaggga
    13681 ttccatctgg taatccgcca tcgcaatggt cgtggagttg aaggtgttgg tttcgatgat
    13741 atccgcgccc gcttcaaagt aggcgttgtg gatagcggcg atcacttccg gtttgcttag
    13801 caccagcaga tcgttattgc ctttgaggtc gcatggccag tcggcaaagc gttcaccacg
    13861 aaaatcggct tcgttcagtc gatagctctg gatcatggtg cccataccgc cgtccagcac
    13921 cagaatacgt tcatttaact gcgcacgcag ttgttccact ttgctgctca cacttgctcc
    13981 cgacacgctc aacccagatt taataaaaat tcaacaaacc atactggcat aaacgcatct
    14041 gtggtaaaag cgaccaccac gcaacatgag atttgttcaa cattaactca tcggatcagt
    14101 tcagcaacta ttgcattagc taacaataaa aatgaaaatg atttccacga tacagaaaaa
    14161 ggagactgtc atggtcgcac ccattcccgc gaaacgcggc agaaaacccg ccgttgccac
    14221 cgcaccagcg actggacagg ttcagtcttt aacgcgtggc ctgaaattac tggagtggat
    14281 tgccgaatcc aatggcagtg tggcactcac ggagctggcg caacaagccg ggttacccaa
    14341 ttccacgacc caccgcctgc taaccacgat gcaacagcag ggtttcgtgc gtcaggttgg
    14401 cgaactggga cactgggcaa tcggcgcaca tgcctttatg gtcggcagca gctttctcca
    14461 gagccgtaat ttgttagcaa ttgttcaccc tatcctgcgc aatctaatgg aagagtctgg
    14521 cgaaacggtc aatatggcgg tgcttgatca aagcgatcac gaagcgatta ttatcgacca
    14581 ggtacagtgt acgcatctga tgcgaatgtc cgcgcctatc ggcggtaaat tgccgatgca
    14641 cgcttccggt gcgggtaaag cctttttagc ccaactgagc gaagaacagg tgacgaagct
    14701 gctgcaccgc aaagggttac atgcctatac ccacgcaacg ctggtgtctc ctgtgcattt
    14761 aaaagaagat ctcgcccaaa cgcgcaaacg gggttattca tttgacgatg aggaacatgc
    14821 gctggggcta cgttgccttg cagcgtgtat tttcgatgag caccgtgaac cgtttgccgc
    14881 aatttctatt tccggaccga tttcacgtat taccgatgac cgcgtgaccg agtttggcgc
    14941 gatggtgatt aaagcggcga aggaagtgac gctggcgtac ggtggaatgc gctgactttt
    15001 tctggcgggc agaggcaata ttctgcccgt catacctgag tggtaataga ataagggtgt
    15061 ctgttaatcg cattgacgtc aaaataactt att
//
LOCUS       DAZFDC010000115        15046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFDC010000115 DAZFDC010000000
VERSION     DAZFDC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.115"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(200..300)
                     /gene="ykgR"
                     /locus_tag="ACHJ48_003671"
                     /pseudo
     CDS             complement(200..300)
                     /gene="ykgR"
                     /locus_tag="ACHJ48_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
     gene            complement(774..1916)
                     /locus_tag="ACHJ48_003672"
     CDS             complement(774..1916)
                     /locus_tag="ACHJ48_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFO4709148.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            complement(2151..3071)
                     /locus_tag="ACHJ48_003673"
     CDS             complement(2151..3071)
                     /locus_tag="ACHJ48_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4709149.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            3228..4154
                     /locus_tag="ACHJ48_003674"
     CDS             3228..4154
                     /locus_tag="ACHJ48_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4709150.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAVSEMGITLAALSQNLKS
                     LEERLNTKLIYRTTRNISLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSKVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            complement(4442..4798)
                     /locus_tag="ACHJ48_003675"
     CDS             complement(4442..4798)
                     /locus_tag="ACHJ48_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709151.1"
                     /translation="MNKNLILAFALFSLPVFAEEDLGPGKYVCDIRISSLDTATQILS
                     KSATVLDNGNNFIVQMPNGDQLYSPDLENVDDGIKQKATIGGVTFIRRPTFNDRFIVE
                     DGNTGFFYKMRNCEKK"
     gene            complement(5006..5566)
                     /locus_tag="ACHJ48_003676"
                     /pseudo
     CDS             complement(5006..5566)
                     /locus_tag="ACHJ48_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            6132..10385
                     /gene="fdeC"
                     /locus_tag="ACHJ48_003677"
     CDS             6132..10385
                     /gene="fdeC"
                     /locus_tag="ACHJ48_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO4709152.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSAAVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDTYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGNAKQTYKVTVTDANNNLLKDSEVTLTASPANLVLTPNGTAKTNEQGQA
                     IFTATTTVAAKYTLTAKVSQADGQESTKTAESKFVADDKNAVLTASSDVTSLVADGIS
                     TAKLEVTLMSANNPVGGNMWVDIKTPEGVTEKDYQFLPSKNDHFVSGKITRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMN"
     gene            complement(10506..11363)
                     /locus_tag="ACHJ48_003678"
     CDS             complement(10506..11363)
                     /locus_tag="ACHJ48_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4709153.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIAISLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            11612..12481
                     /locus_tag="ACHJ48_003679"
     CDS             11612..12481
                     /locus_tag="ACHJ48_003679"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO4709154.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(12641..13234)
                     /gene="rclC"
                     /locus_tag="ACHJ48_003680"
     CDS             complement(12641..13234)
                     /gene="rclC"
                     /locus_tag="ACHJ48_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO4709155.1"
                     /translation="MEKYLHLLSRGDKIGLALIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQGSNESSSTLKTEY"
     gene            complement(13246..13482)
                     /gene="rclB"
                     /locus_tag="ACHJ48_003681"
     CDS             complement(13246..13482)
                     /gene="rclB"
                     /locus_tag="ACHJ48_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO4709156.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(13591..14916)
                     /gene="rclA"
                     /locus_tag="ACHJ48_003682"
     CDS             complement(13591..14916)
                     /gene="rclA"
                     /locus_tag="ACHJ48_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO4709157.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVINGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         4115 a         3249 c         3556 g         4126 t
ORIGIN      
        1 gtgattgatg atagtttcgc tgagagtatg gatttttcga gaatggaggt tatctccact
       61 ttactggccg atgatttatc gcagtatgat tgattacctg acagcgtgta ttcctctccc
      121 acggatataa taattactta caaaaaggga gaggatgcat attttaaata tcactgaagt
      181 gaacagttta ttgccgttat taatagaaat ggagaaataa ataggcgtat tctacaattg
      241 cgacaaaaca acgatattaa tcagtttatg actgatttgt tgtactttat tctctttcat
      301 tggtacttcc tcgctttaaa aaagagcgca cttcgtaagt gcccttatat aaataacgag
      361 tttggtcaac caattttttg acatgtatca caaatttgaa tagatgtatt acatcaggca
      421 tcttttattg ccccaacgtc attgatatat gtcgcctgaa gtcagttccg ggaatgagtc
      481 tgatctcaag actggcccag tcctggcgtt gattggcgct gaggagcata tcgcatctca
      541 tcataatgtc gtatctcctg gggtgttata caagatatcg ttgttggtaa cctgggcgag
      601 gaattgagct ctattaaacc gtcaactatg ccggatacat actagattac actgcaggca
      661 cgccttatga gagaacgtgc cgcagtgacg ggttaattat ctgaaagaat ttgtgaggct
      721 gtatcggtta ctcattgatt tgatagtttt actctcggga gagtaataga tatttaatcc
      781 attaacggaa accagccagt tcctttcgat gcctgaattt gatcccatag tttatccgga
      841 atggttaatt caggaacaag ttgtggatta agggttgttt gaatttcatg gcctctacct
      901 gattcagcta aagtcaccca ttctggattt atcaccagcc ctttaccgat cgcaacaagt
      961 ggcaggccag tggaaattgc ttcttgagcc tgagacggtg tgcgtatttt acctgcagcg
     1021 attaccggca ctctgcctgc aatatgattt aaaatcaatt caatagttcg ggggccattt
     1081 ggcgactcaa cagggtagct atcgttaata ctgaccagcg aggtatgtat atacgaaata
     1141 ccggatgaaa tgagccgatc aaggagttta taagtatcct ctatccgcag cccccctgtt
     1201 gcagattctt caggagatat tctgtaaccg atggcaaagg gctttgtcgc atattcatag
     1261 actacatttt ttacttcctg taatacggcc agcgggaagc gcatacgtcc ttcgagatct
     1321 cctccccatc ggtcattacg ctggttaaat agaggggaga aaaaattctg tagaaggaat
     1381 ccgtgcgcac catgaagttc aatgccatca aaacctgctt taatcgccct ttttgtgaca
     1441 tctccgaatg ctctaatggt ttcctgaatt tcgttttcag tcatttccct gctttgtacc
     1501 actctcttca taaagtcacc agattttacg cttgatgcgc tggcactaat aacatcgtta
     1561 tttggtacaa gctccgggat ggctttatta ccggcgtgga aaatctgcaa aatagccgga
     1621 gcaccgccac ttttggctgc cgctgctaac ttttcaaggc tgttaatgaa acggtcgtca
     1681 tacgcggcaa attcatgagt aaacccaatg ccgcttggcg tgacataggt acatcctgtt
     1741 attactaaac caacattttg tgagcgacgt ttataaaatt cgagttcttg ctcagagatg
     1801 gttccatcag gattcgctga ccaggtcgtc ataggagcca taacgatacg attacgtagc
     1861 tttatttttt ctgtcagcat aaaaggagaa aacaatgatg ggtgtttatt tgtcataaca
     1921 atacctcaaa tgtttatctg attttgatgc gtatgtttta actggttagt tgttgccagc
     1981 tttggaacca agagttctgg ctttatttat aatcccttta aagggtttct aataattgag
     2041 ttgctgcttt tttgtttttt aatgaaattt ctttttgaat aattttatta ttgttgtgta
     2101 gtcaataaag gataaatgtc agggagataa gggatctccc tgtaaaatta ttacaaatgt
     2161 gttttataaa aaggaattaa tttatcgagt gcctgtttca caggttctgg cttatcatac
     2221 aggtcgtaat gtgaataacc ttcaaccacg accagctctt tatgttttga agcagcgcgg
     2281 ccaataattt cgcagccgtc acggtaggca ccaaatgcgc ccactctatc gcccaccaca
     2341 accattattg gttgagttaa cagcacttct gcaagatgaa atgcatccca acccacggca
     2401 acagtctgat gtgagaatag cgaacgattt accccattag gtgcatagcc tcgtggcgaa
     2461 cgataatatt ctgtggcttc aaaaaggtct atttctgtta gtcccgcttc aagagctgca
     2521 tccggagaag aggggagtaa atcatctacg cgaagttttg cgccgttagc ttcgtctgtt
     2581 ctttgttgcg ccattgcttc aagtgctccg ataggattaa acgctgtaaa tccctcacgc
     2641 ataagacgcc cgtagtttgc acccgtgaca gtaccaatag ctttaattcg gcgttctgtc
     2701 attgcggcat taatggcata tccacctcca ccacaaatac ccagtacacc aatacgcccg
     2761 gcatcaacgt aaggtaagct ggtaaggtaa tcaacgacaa tgctgaagtc ttttactcgc
     2821 attgttggat cttccagata acgcggttcc ccaccgctgc ttccctgaaa actagcatca
     2881 aaagcaatga cgataaagcc agcttttgcc agggcggcac catacacact acccgatgtc
     2941 tgttctttgc agcttccaat tggatgggcg ctgataatgg caggatactt tttttcacta
     3001 ttaaaatcgt cagggaaata gatatcagca gctatatccc agtaagtgtg ttttatcgaa
     3061 acagttttca tttcaagctc cggggatttg cctaaacaga tttaaatatc atttctatta
     3121 tccgcaactg gcatttgtgt tcatatcgag aacggcgtaa tagtatgact tgtattggtt
     3181 atcatcaatg gtctaatatc tatcacagtg ttaagttaag gtttacaatg atgaaaatag
     3241 agccttcaat tttgccttct cttgcctggt ttgcgctgat tgttcgtgcc ggcagttttt
     3301 ccagagccgt ttctgaaatg ggtattacgc tggccgcatt gtcccagaat ttaaaatctc
     3361 ttgaggaacg tcttaacacc aagctgatat accgtacgac ccgcaatatc tcgcttactg
     3421 aggaaggaca gcgtctgtat gaagtgctgg tgtcggcttt ggggcaaatt gatgatgctt
     3481 tgaaagatgt tggtgatacc caacttgaac ctacaggcct gttgagaata aattcctccc
     3541 gtgttgcagc acggatgctt gttgagcctc atattggcga gtttcttact cgttacccca
     3601 aaacaaagat agaactcatt atggatgacg gtttatctaa cattattgcg gacggctgtg
     3661 atgtgggcat acgtcttgag caaggactgg atgagcatat gactgccgtt cctgtttctc
     3721 cgcttattaa actggttact gtggcatcgc cagattattt aaaagaacac ggtattcctg
     3781 aaacgcctca ggaactgagt aaccataatt gccttcggtt gcggcataaa agtagcggtg
     3841 cactctctgc atgggagttt tctaaggttg tggggggtaa tgaagagttc gaaatagagg
     3901 tttcaggtaa gtatatttct aatgatgacg aaagcatgat acgaatggcg ctgaatggaa
     3961 cgggtatcat tcaacatctg gattttgcaa tcgctgaaca tatcaatgcg ggcaagttac
     4021 agccgattct tgaggattgg gctgtttcat ttcctgggtt ttatatttat gtttcatcac
     4081 gggtgagaat gccctctaaa gttcgtgctt ttattgattt tatggtggag aaaagagtaa
     4141 aactcgaaaa ttgatgatat gtgtaattga tggttgctct gggcgaatat cggtaagagg
     4201 aataacgtaa tcattaggat gcgtttacat aataaagttt ctgataatta atacttattt
     4261 tctatacggc cataatatca attatcatta tcaccagtaa tgtggttaaa ataatagtat
     4321 ctatgaccgc tacggttaac ggtggttatt ttgggaggcg ttaacacgta gcggcttgta
     4381 gttgtaagtc ttgaacaggt acaaagcctg aaatcaggag gatatcgcta tcaaacttcc
     4441 tttatttctt ctcgcagttt cgcatcttat agaagaatcc tgtatttcca tcttccacga
     4501 tgaagcgatc gttaaaagtt ggacgacgaa taaatgtcac tccacctatt gttgcttttt
     4561 gttttatacc atcatcaaca ttttccagat ctggagagta caattgatct ccatttggca
     4621 tttgaacgat gaaattgttt ccattatcga gaactgttgc ggattttgac agaatttgag
     4681 tagcggtatc caggctggaa atcctaatgt cacaaacata tttgcctggc cccagatctt
     4741 cttctgcaaa tacaggtaag gaaaataatg caaatgcgag gatgagattc ttattcatgc
     4801 aaactccagg aagatcaata aaagcaatat gttacataaa aagcacgtaa atgaaaagag
     4861 cgattggtta atgagtaatg ctattaatcc ccgcatggcc tacttttcat tggctgcggt
     4921 gttgaacctc attgaatatg ggcaagactc tgactgagtg accgacgtaa ataggtagat
     4981 gcataatcgc ttaccgccct gtaactgcaa acggtgccca ggcttctggc tgaataccac
     5041 ggctttgcat ccacggaacg gcgtgtagct gctgattgaa agggttaact tcaacctggt
     5101 ttaccgctgg aacgacgtta ttaaaggcga taaggtcagc cagtcggtca ggatggaagt
     5161 tactgacgcc aatagcgcga attttaccgg cctgttgcag ctcttccatg gcacgccagg
     5221 caccgtggac atcaccgtaa ggctgatgaa tcaaatacag gtcaacgtaa tcaagttgca
     5281 gtcgattcag agagcgttcg aactgggctt tggccccttc gtaactggta tcctgcaacc
     5341 agagtttcgt ggtgacaaag agttcgttac gggcgatacc gctttgtttc agtgcgttcc
     5401 cgacctgggg ttcattttga taggaggcgg cggtgtcaat cagtcgataa ccggtgttaa
     5461 tagcgtcaat gaccgctctt tcgcattcgg cggcatcggt catctgaaat acaccaaagc
     5521 ccagcagggg catttcaata ccgttattca gttttacttt ttgcatggtg ttatccttcc
     5581 gttatcgttc atgagaagca taacgtaaag agaagagctc gattagacgg caggatttgt
     5641 caggggttat gaacgaaatt cataaatctg tttgagtgtt gcgatgggta gtgcaagttc
     5701 gatatctccg caatttacag tccgatgaag gaaaatgaat atccataaaa aatatatcgg
     5761 tttatcctgg cgtatatacc tacttcgacg tatttccaat agttgctaat taaaggcagg
     5821 tcattattat tcactctgaa taatgaatta ttcactgtcc gcagagtaag aaatataact
     5881 taagtatcta tttaatgact tgcacaaaaa gctaaatttt cacccataaa taaaaatata
     5941 atcccgcgcc caaccacctg atgagtggct acaggcactg gatatattag gtggcggtgt
     6001 actttcttac ataaaggtat ttccttttct gcggaaaagg aaaccgggaa atccccggtt
     6061 tttctgacaa gcagacgcca ttatttgtgt ctgcctatgt tcgttaattc gttcatcagg
     6121 aaattatctc aatgtcacgt tataaaacag gtcataaaca accacgattt cgttattcag
     6181 ttctggcccg ctgcgtggcg tgggcaaata tctctgttca ggttcttttt ccactcgctg
     6241 tcacctttac cccagtaatg gcggcacgtg cgcagcatgc ggttcagcca cggttgagca
     6301 tgggaaatac tacggtaact gctgataata acgtggagaa aaatgtcgcg tcgtttgccg
     6361 caaatgccgg gacattttta agcagtcagc cagatagcga tgcgacacgt aattttatta
     6421 ccggaatggc caccgctaaa gctaaccagg aaatacagga gtggctcggg aaatatggta
     6481 cagcgcgcgt caaactgaat gtcgataaag atttctcgct gaaggattct tcgctggaaa
     6541 tgctttatcc gatttatgat acgccgacaa atatgttgtt cactcagggg gcaatacatc
     6601 gtacagacga tcgtactcag tcaaatattg gttttggctg gcgtcatttt tcaggaaatg
     6661 actggatggc gggggtgaac acctttatcg accatgattt atcccgtagt catacccgca
     6721 ttggtgttgg tgcggaatac tggcgcgatt atctgaaact gagcgccaat ggttatattc
     6781 gggcttctgg ctggaaaaaa tcgccggata ttgaggatta tcaggaacgc ccggcgaatg
     6841 gttgggatat ccgcgcagag ggctatttac ctgcctggcc gcagcttggc gcaagcctga
     6901 tgtatgaaca gtattatggc gatgaagtcg ggctgtttgg taaagataag cgccagaaag
     6961 acccgcatgc tatttctgcc gaggtgacct atacgccagt gcctcttctg acactgagcg
     7021 ccgggcataa gcagggcaag agcggtgaga atgacactcg ctttggcctg gaagttaact
     7081 accgaattgg cgaacctttg gcgaaacaac tcgatacgga tagcattcgc gagcgtcggg
     7141 tactggcagg cagccgctat gacctggttg agcgtaataa caacatcgtt cttgagtacc
     7201 gcaaatctga agtgatccgt attgctctgc ctgagcgtat tgaaggtaag ggcggtcaga
     7261 cactttccct ggggcttgtg gtcagcaaag caactcacgg actgaaaaat gtgcagtggg
     7321 aagcgccgtc attactggct gaaggtggca aaattaccgg tcagggtagt cagtggcaag
     7381 taacgctccc ggcttatcgt ccaggcaaag acaattatta tgcgatttca gcagttgcct
     7441 acgataacaa aggcaatacc tcaaaacgcg tgcagacaga ggtggtcatt accggagctg
     7501 gtatgagcgc cgatcgcacg gcgttaacgc ttgacggtca gagccgtatt caaatgcttg
     7561 ctaacggtaa tgagcaaaaa ccgctggtgc tgtctctgcg cgacgccgag ggccagccag
     7621 tcacgggcat gaaagatcag atcaagactg aactaacttt caaaccggct ggaaatattg
     7681 tgactcgttc cctgaaggcc actaaatcac aggcaaagcc aacactgggt gagttcaccg
     7741 aaactgaagc aggggtgtat cagtctgtct ttactaccgg aacgcagtca ggtgaggcaa
     7801 cgattactgt tagcgttgat ggcatgagca aaaccgtcac tgcagaactg cgggccacga
     7861 tgatggatgt ggcaaactcc accctgagcg ctaacgagcc gtcaggtgac gtggttgctg
     7921 atggtcagca agcctatacg ttgacgttga ctgcggtgga ctccgagggt aatccggtga
     7981 cgggagaagc cagccgcttg cgatttgttc cgcaagacac taatggtgta accgttggtg
     8041 ccatttcgga aataaaacca ggcgtttaca gcgccgcggt ttcttcgacc cgtgccggaa
     8101 acgttgttgt gcgtgctttc agcgagcagt atcagctggg cacattacaa caaacgctga
     8161 agtttgttgc cgggccgctt gatgcagcac attcgtccat caccctgaat cctgataaac
     8221 cggtggttgg ggggacagtt acggcaatct ggacggtaaa agatgcctat gacaaccctg
     8281 tgaccagcct cacgccggaa gcgccgtcat tagcgggtgc cgctgctgaa ggttctacgg
     8341 catcgggctg gacaaataat ggtgatggga cgtggactgc gcagattact ctcggctcta
     8401 cggcgggtga attagaagtt atgccgaagc taaatggaca gaatgcggca gcaaatgcgg
     8461 caaaagtaac cgtggtggct gatgcgttat cttcaaacca gtcgaaagtc tctgtcgcag
     8521 aagatcacgt aaaagccggc gaaagcacaa ccgtgacgct ggtggcgaaa gatgcgcatg
     8581 gcaacgctat cagtggtctt gcgttgtcgg caagtttgac ggggaccgcc tctgaagggg
     8641 cgaccgtttc cagttggacc gaaaaaggta acggttccta tgttgctacg ttgactacag
     8701 gtggaaagac gggcgagctt cgcgtcatgc ctctcttcaa cggccagcca gcagccaccg
     8761 aagccgcgca gttgacggtc atcgccggag agatgtcatc agcgaactct acgcttgttg
     8821 cggacaataa ggctccgacc gtcaaaacga cgacggaact caccttcacc gtgaaggatg
     8881 cgtacgggaa cccggtcacc gggctgaagc cagatgcacc agtgtttagc ggtgccgcca
     8941 gcacggggag tgagcgtcct tcagcaggaa actggacaga gaaaggtaat ggggtctacg
     9001 tgtcgacctt aacgctggga tctgccgcgg gtcagttgtc tgtgatgccg cgagtgaacg
     9061 gccaaaatgc cgttgctcag ccactggtgc tgaacgttgc aggtgacgca tctaaggctg
     9121 agattcgtga tatgacagtg aaggttaata accaactggc taatggacag tctgctaacc
     9181 agataaccct gaccgttgtg gacacctatg gtaacccgtt gcaggggcag gaagttacgc
     9241 tgactttacc gcagggtgtg accagcaaga cggggaatac agtaacaact aatgcggcag
     9301 gtaaagcgga cattgagctt atgtcaacgg ttgcgggaga acacaatatt tccgcttcgg
     9361 tgaatggtgc tcagaagacg gtcacggtga aattcaacgc ggatgccagc accggtcagg
     9421 caaacctgca ggtagacgcc gctgctcaaa aagtggcaaa cggcaaagat gcctttacgc
     9481 tgacggcgaa cgttgaggat aaaaatggta accctgttcc agggagcctg gtgaccttta
     9541 atctgccccg gggtgtcaag ccgcttacag gcgataatgt ctgggtgaaa gccaacgatg
     9601 aggggaaagc agagttgcag gtggtttcag tgactgccgg aacgtatgag atcacggcat
     9661 cggcagggaa tagccagcct tcgaatacgc agactataac gtttgtagcc gataaggcta
     9721 ccgcaaccgt ctccggtatt gaggtgattg gcaactatgc actggcggac ggcaatgcca
     9781 aacagacgta taaagttacg gtgactgatg ccaataacaa cctgttgaaa gatagcgaag
     9841 tgacgctgac tgccagcccg gcaaatttag ttctgactcc caatgggacg gcgaaaacta
     9901 atgagcaagg acaggctatt ttcaccgcca cgaccactgt cgcagcgaaa tatacactca
     9961 cggcgaaagt gagtcaggcc gacggtcagg aatcgacgaa aactgccgaa tctaaattcg
    10021 tcgcggatga taaaaatgca gtactcaccg catcatctga tgtgacttct ctggtggcgg
    10081 atgggatatc gactgcgaag ctggaggtga cactgatgtc ggcaaataac cccgttgggg
    10141 ggaatatgtg ggtcgacatt aagacgccag aaggggtgac ggagaaggat tatcagttcc
    10201 tgccgtcgaa aaatgaccat ttcgtgagcg gaaaaatcac gcgtacattt agtaccagca
    10261 agcctggtgt ctatacgttc acatttaacg ccctgacgta tggcgggtac gaaatgaagc
    10321 cagtgacggt gaccattacc gcggtggatg ccgatacggc aaagggcgag gaggcgatga
    10381 actaatatta agaatatgga agtaacattc taataaacaa taatggcgtt gacatcttca
    10441 acgccattat ttattgaatt gcctgacagt agtctcgtta taagttcatt tagatatagc
    10501 gacagctaaa ccggacgaac agggataaat atttcgcaaa caacatcatt gctatccttt
    10561 tctacgaaat cttcattgta ataaaaccgc tcaatatcac tgccgttcac tctggctaac
    10621 cccctggcgg gtaattccat cagatagagg tttcgcgccc attcagggta gtgctcccac
    10681 tcaccttcat agcggaaaga ggcatacagt cctcggttaa ttgtccactc tttcggcatc
    10741 tctgaatcgg tttgtttgca taccgtaccg gcaaaagttt caactgtgag taaatcgacc
    10801 gattttgttg acggttcaaa cgtggagacg aaatagatat ccgtcttaaa attcttcata
    10861 catctggcta aatgatgacg tcgcagtttg acttcttcat ccggtgattt attcgacact
    10921 tcaatatcat atttaaatga attgccaaaa ataggtgtct caggaaagtt gactaatcta
    10981 cactccgtct tttgctgttg acgtattaaa aaagaaggga agatatttgc gagatcccaa
    11041 taatcacggt ggcggtatac acggggagag cagccgaata actttttaaa ttcgcggctg
    11101 aatgactgct gtgaatcaaa atgcaaagag attgcaatat caagcataga tttcgcggtg
    11161 agccggacaa gaatggcggc acgacaaagc cttcgtttgc gaatgtattc tcccaaaggc
    11221 acatgcatga aatttcggaa cagaagctgg atgttgcgtc tgctgtagcc agatttctgt
    11281 gcgatatctt cgattgaaat agggtgctca agattgcact caatccactc caggagctgc
    11341 tgtagaatct tctgcctgat catcacattc tccgttaatt ctttgtcata tcgcacattt
    11401 tcgttatgcc ctgggcccta agtgtatcaa aaacagcaag ggcgaaggca aaagcactgg
    11461 tagcgtctgc ttcgttgtcc ccgaaaatag agaggtttca gtcctacatt attaatgaat
    11521 tttttgcata agtgatatca aaatccacat actaatttga ggttacgttt taacgtagac
    11581 tcattgttca tgcctaatgg agggactgac agtggaattt tccgtattaa gtaacaatct
    11641 gaaaatgccg atgatgggat ttggtgtttt tcaggttacc gataaaaacg tgtgcaaaca
    11701 gtcagtgttg aacgctatcc gcacggggta tcgactcatc gacactgcag cggtatacgg
    11761 taatgaagat gccgtcggtg aagccgttcg tgaagctata tccgaaggtt tatgtacccg
    11821 agaagagtta tttataacgt ctaaattgtg ggtgcaggat atgttgaatc aggatacggc
    11881 agcagcaggt attgaagcat cattaaaaaa atcgggacta gaatacttcg acctctattt
    11941 attgcatcag gctatgcgcg attatttcag tgcgtggcgt gcactcgaag atgcctatga
    12001 agagggcaaa ttaaaagcga ttggggtttc caatttctat cctcatgttc tggcgaactt
    12061 ttgtgaaacg gtaagagtta aaccgatggt caaccaggtc gagttgcatc cctattttgc
    12121 ccaaccagag gcgctggcaa ccatgaagta ttataacgtg cagcctgaag catgggctcc
    12181 gttaggtggt ggacgacata aaccctttga aaataatctg cttcagagta ttgcagatgc
    12241 ccatcaaaaa tcgatttctc aagtcattct gcgttggaat attcaacggg gagtggtcgt
    12301 tattccgaaa tcgacacatc aacagcgtat cgaagaaaat tttgctatct gggatttctc
    12361 actgacagag aaagaaatgg cacaaattag ttcgcttgat ttgggttatg ttggggagtc
    12421 ggtaaaacat tttaatcctg aatttgttcg tggttgtctt gctgtaaaaa tacatgattg
    12481 atattaatca tgtattttac ctgagacgac aagaatcttt taacagggga gtgatattga
    12541 tcttcactct gtcatatctc cggtaatatg gcgtcaggct tcatcatcag aaagggggcc
    12601 tgacgcctga aaaagtgaac aacagacagt gttcggatta tcaatattca gtttttaacg
    12661 ttgaactgga ttcattactg ccttgtttaa gaatttcccg cgccgaatct gccattatca
    12721 tgacagcacc tgccagcatc agagtatctt tcaataccag acgaccagca ccggataaat
    12781 aagggaaacc atgatgagcg tcacccaatg cgggtaccca tgcctccggg gtggtgatta
    12841 aaaatgagag tgttaccagc ggtgtggtaa atgccatcag ccctcccaat aaacctaacc
    12901 agcgattgac aggattagcc aaaaccagca gagcaataat cacctccacg acgccaagac
    12961 cgttggaaaa accataggta ttattggccg attgccatgc ccttgcttct ggtttgtatt
    13021 cgccttcgtg agtcagatac tgtttatagt cttccgggtg ttcataaaag aacgacatta
    13081 gtggactgtt tgcgacgaat ggtgtaatgc tgtctgcctc gtaagggaca aactttaata
    13141 acccaatcca cataaaaaca atcgcaatac tcagacgaat caatgccagg ccaattttat
    13201 ctccccgact taacaggtgc aggtattttt ccattgtgaa tatccttatt tatacaatac
    13261 tgctgttgcg gttgaattat tatcaattcg catcgatgtg actttccagg atgaagcatt
    13321 atattttctg gctatatcag caatggcatc ctccagaact gtcggtgatg ctgatactga
    13381 cgagacgctg atatgtttca gaattatttt atcatctgca ttggtggaaa atgccataac
    13441 gccagataat agtgttgcaa ataaaacgga ttttttaaac atataaaata ccatcaatga
    13501 agtgattaag aagttataga gtaacaggga attaataaga ttcttttctc tgagacgcca
    13561 gaatatttgt tctggcgtct gattttgagt ttatttgact aatgaaaata gatcattgag
    13621 tgattcgctc atcgacggat gagtaaatat ctgatcgcgt aatatgctat aaggcagccc
    13681 ggcatccatc accattttca ctatattaat catctcgtgg gagtcaacac acagcagtga
    13741 tgcccctaat atacgttggg ttttattatc aacaatcgct tttaataccc cacgggtatc
    13801 gttcatcact ctggcgcgcg gaattgcagc tacaggcaat gtcaccacct gaatatcagc
    13861 accactctct ctggcttgtt cttctgtcat accaaccctg gacaggggcg gtgtcataaa
    13921 tacggaataa ggcacatttt tccgatcatc agtactacgt ttgccttcac ccagtaactc
    13981 atcacgtaca atgcggtaat catccagtga tatgtaagta aattgcagcc cgccggtaac
    14041 atctcccatc gcccaaatat tgtctgcggt ggtatgtaat cgcttgtcaa cgacaattgc
    14101 cccgcgctcg tttactgcga taccggcatt ttctggatgt aacgaagcgg tagccggttg
    14161 acgaccggaa gctattaaca gtgcatccac cgccagttgg gcgtgctcgc tatgcacttg
    14221 cacttgattt tcatggtgac tgattcgctc cacatgggca ttgaggataa tatcgacgcc
    14281 ctgatcgcgt aaaatcgtcg cgatattatc agcaatatcc cgttcttccc gaggcaaaaa
    14341 cagcgaagct gcttctaaaa tggttacctt gctgccaaaa ttagcgaaca tagaggcgaa
    14401 ctcaacgcca atatatccgc cgcccaaaat acctaaatgc ccaggcaatt cttttagatt
    14461 aagtaatccg gtgctgtcat atactcctgg cgtggtggta attccaggaa ttggcggaac
    14521 cacggtttgt gcaccggtat taataaaaat tttctcgcca tgaatttcca gatttccctc
    14581 aggccgatga acacgcaggc tatgattatt gataaactcc gcctggccgt tgatcacgtc
    14641 gatattgggc atatctgcaa gattatgaaa attcttatta cgtaaaaaat taaccacttc
    14701 atttttacgc tgtatggctc ggacaaaatc tgtgtgctgc tgtgcgtcat gaaccaatgt
    14761 tttggttggg atgcagccga tattaataca ggtcccgcca tacattgcat ttgattgttc
    14821 gatgagagcc actcgccaac ctgcttttgc cagcgtgacg gctaatgttt ttccagcctt
    14881 gccaaaacca ataatcactg cctgatattt attcatgatg atttcctgtc gaaacgcgtt
    14941 catttgacat catgactata gacgcaaaag agcaaataac atttcttcac aacgcttgat
    15001 attttgtctt atcgtctggt attttctttt tcttaatcag aatcac
//
LOCUS       DAZFDC010000116        14958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFDC010000116 DAZFDC010000000
VERSION     DAZFDC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.116"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            139..381
                     /gene="yjbE"
                     /locus_tag="ACHJ48_003683"
     CDS             139..381
                     /gene="yjbE"
                     /locus_tag="ACHJ48_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO4709158.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            495..1133
                     /gene="yjbF"
                     /locus_tag="ACHJ48_003684"
     CDS             495..1133
                     /gene="yjbF"
                     /locus_tag="ACHJ48_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO4709159.1"
                     /translation="MKRPAFILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWMRTMGWTEYQQVRYATARSVFKWDGTGTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSGGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1130..1867
                     /gene="yjbG"
                     /locus_tag="ACHJ48_003685"
     CDS             1130..1867
                     /gene="yjbG"
                     /locus_tag="ACHJ48_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO4709160.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLGGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            1867..3963
                     /gene="yjbH"
                     /locus_tag="ACHJ48_003686"
     CDS             1867..3963
                     /gene="yjbH"
                     /locus_tag="ACHJ48_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO4709161.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERANTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIVRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4010..4288)
                     /locus_tag="ACHJ48_003687"
     CDS             complement(4010..4288)
                     /locus_tag="ACHJ48_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709162.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSALPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4289..4432
                     /locus_tag="ACHJ48_003688"
     CDS             4289..4432
                     /locus_tag="ACHJ48_003688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709163.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4558..4968
                     /gene="psiE"
                     /locus_tag="ACHJ48_003689"
     CDS             4558..4968
                     /gene="psiE"
                     /locus_tag="ACHJ48_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO4709164.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQASKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVVTLWLCNSKRLKRE"
     gene            complement(5012..6487)
                     /gene="xylE"
                     /locus_tag="ACHJ48_003690"
     CDS             complement(5012..6487)
                     /gene="xylE"
                     /locus_tag="ACHJ48_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO4709165.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6859..7749)
                     /gene="malG"
                     /locus_tag="ACHJ48_003691"
     CDS             complement(6859..7749)
                     /gene="malG"
                     /locus_tag="ACHJ48_003691"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO4709166.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7764..9308)
                     /gene="malF"
                     /locus_tag="ACHJ48_003692"
     CDS             complement(7764..9308)
                     /gene="malF"
                     /locus_tag="ACHJ48_003692"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO4709167.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGREQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9462..10652)
                     /gene="malE"
                     /locus_tag="ACHJ48_003693"
     CDS             complement(9462..10652)
                     /gene="malE"
                     /locus_tag="ACHJ48_003693"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO4709168.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11017..12132
                     /gene="malK"
                     /locus_tag="ACHJ48_003694"
     CDS             11017..12132
                     /gene="malK"
                     /locus_tag="ACHJ48_003694"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO4709169.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12204..13544
                     /gene="lamB"
                     /locus_tag="ACHJ48_003695"
     CDS             12204..13544
                     /gene="lamB"
                     /locus_tag="ACHJ48_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO4709170.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13695..14615
                     /gene="malM"
                     /locus_tag="ACHJ48_003696"
     CDS             13695..14615
                     /gene="malM"
                     /locus_tag="ACHJ48_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO4709171.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14844..>14958
                     /locus_tag="ACHJ48_003697"
     CDS             14844..>14958
                     /locus_tag="ACHJ48_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709172.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDFDLLRNFNGGP"
BASE COUNT         3689 a         3842 c         3945 g         3482 t
ORIGIN      
        1 ttgatattca ttccaattat cctaaaaccc catctctaat tccccgcgcc gtaattcgca
       61 tgctttagtt gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc tccattcatt
      121 caatgaaggg aaattgttat gaaaaaagtt ctgtatggca tttttgccat atctgcgctt
      181 gcggcgactt ctgcgtgggc tgcacctgta caggtgggcg aagcggcagg gtcggcagca
      241 acgtcggttt cggcggggag ttcctccgcg accagcgtca gcaccgtaag ctcggcggtg
      301 ggtgtcgcgc tcgcggcaac cggtggcggt gatggttcta ataccgggac caccacaacc
      361 acgaccacca gtacccagta ataaagtatg tatccccaaa ataattcgag tcattgcatc
      421 tgtggctaga agtatgaagg gatttaccat aaccacactc cggtgtggtt attctgcccc
      481 tctggagaag agtcgtgaag cgacctgcat tcattcttat ctgcctgcta ttacaggcct
      541 gttcagccac gactaaagag ctgggcaatt cactgtggga cagtctgttc ggcacgccag
      601 gcgtacagct gacggacgat gatattcaaa atatgcccta cgccagccag tacatgcagc
      661 ttaatggtgg gccgcagttg tttgtggtgc tggccttcgc tgaagacgga caacaaaaat
      721 gggtcactca ggatcaggcc actctcgtca cacaacatgg tcgtctggtg aagactttgc
      781 ttggcggcga caacctgatt gaagtgaata accttgccgc cgacccgctg attaaacccg
      841 cacaaatcat tgatggcgca acctggatgc gcacgatggg ctggaccgag taccagcagg
      901 tacgctacgc caccgcacgc tcagtcttca aatgggatgg caccggtacc gttaaggtcg
      961 gcagcgatga aaccgcggtt cgcgtgctgg acgaagaagt ctccaccgac caggcgcgct
     1021 ggcataaccg ctattggatc gacagcggag ggcaaattcg ccagtcggaa cagtatctcg
     1081 gcgcggatta tttcccggtg aaaaccactc tcatcaaggc ggcaaaacaa tgattaaaca
     1141 aactattgtc gcgttgcttt tgagtgtggg agcgtcatcg gtcttcgcgg caggaaccgt
     1201 caaggtgttc agcaatggca gcagtgaggc caaaacgcta acgggcgcag agcatttaat
     1261 cgatctggta ggccaaccgc ggctggcaaa cagctggtgg cctggtgcgg tgattagcga
     1321 agagctggca acggcggcag cattgcgtca gcagcaggcg ttgctgacac ggctggcaga
     1381 acagggcgca gattccagcg ccgacgatgc cgctgcgatt aacgccttac gccagcaaat
     1441 tcaggcgttg aaggtgacgg gcaggcaaaa aatcaatctt gatcccgata tcgtccgcgt
     1501 tgctgaacgc gctaacccgc cgttgcaggg caactacacg ctgtgggtcg gaccaccacc
     1561 gtccacggtc acgttgttcg ggcttatcag ccgtcctggc aatcagccat tcactcccgg
     1621 tcgcgacgtg gcgagctatc tctccgggca aagtctgctt ggcggtgcgg atcgtagcta
     1681 cgcgtgggtg gtttacccgg acggacgcac acaaaaagcg ccggtggctt actggaacaa
     1741 gcgtcacgta gagccgatgc ccggcagcat tatttatgtt ggcctcgcgg actccgtctg
     1801 gagtgagatg cctgatgccc ttaacgccga cattcttcag actctgacgc agcggatacc
     1861 tcaataatga aaaaaagaca tctgcttagc ttactggcgc tgggcattag cacagcttgc
     1921 tacggcgaaa catatcctgc gcccattggt ccgtcgcagt cggatttcgg tggcgtagga
     1981 ttattacaaa cgcccacggc tcgcatggcg cgggaagggg agttgagtct gaactatcgc
     2041 gataacgatc agtaccgtta ttactcagct tcagtgcaac tcttcccgtg gctggaaaca
     2101 acgctgcgct acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc gttctctggc
     2161 gatcaaacgt ataaagataa agccttcgat ctcaaactgc gtttgtggga agagagttac
     2221 tggctgccgc aagtggcggt tggtgcgcgg gatatcggcg gtacggggct gtttgatgcg
     2281 gaatatcttg ttgccagcaa agcctggggg ccgttcgatt ttacgcttgg cctgggctgg
     2341 ggatatttgg gcacaagcgg taatgtgaaa aatccgctct gttcagccag tgataaatat
     2401 tgctatcgcg ataacagcta caaacaggcg ggatctatcg acggtagcca gatgttccac
     2461 ggtcctgcct cactgtttgg cggcgtggaa taccagacgc cctggcaacc gctgcgcctg
     2521 aaactggagt atgaaggcaa taattatcag caggattttg ccggtaagct ggagcaaaaa
     2581 agtaagttta acgtcggggc gatttatcgc gttaccgatt gggccgacgt taaccttagc
     2641 tatgaacgtg ccaacacctt tatgtttggc gtcacgctgc gcaccaactt taacgatctg
     2701 cgcccgtctt acaacgataa cgcccgcccg caatatcaac cgcagccgca ggatgccatt
     2761 ttgcagcatt cggtggtggc gaatcagtta acgctgttga aatacaatgc cggacttgcc
     2821 gatccgcaga tccaggcgaa aggcgatacg ctgtatgtta ccggcgagca ggtgaaatat
     2881 cgtgattcgc gcgaagggat cgttcgtgct aatcggatcg tgatgaatga tctgccggat
     2941 gggatcaaga cgatccgcgt tacggaaaat cgccttaaca tgccgcaggt gacgacggaa
     3001 acggatgtcg ccagcctgaa aaatcatctc gccggagagc cgttgggcca cgaaacgaag
     3061 ctggcgcaaa aacgcgtcga gccagtggtt ccgaagtcca ccgagcaggg ctggtatatc
     3121 gacaaatcgc gctttgattt ccatatcgat ccggtgctga accagtcggt cggtggcccg
     3181 gaaaactttt acatgtatca gctgggcgtg atgggaacgg cagatttgtg gctgacggac
     3241 catctgctga ccaccggcag cctgtttgca aatcttgcca acaactacga caagtttaac
     3301 tacaccaatc cgccgcagga ctcgcactta ccgcgcgtgc gtacccatgt gcgcgagtat
     3361 gtgcagaacg atgtctatgt gaataacctg caagccaact acttccagca tctgggcaac
     3421 ggcttctacg gtcaggtcta cggaggttat ctcgaaacca tgtttggcgg tgcgggggca
     3481 gaagtgttgt atcgcccgct ggacagcaac tgggcgtttg gtctggatgc caactacgtt
     3541 aaacagcgcg actggcgtag tgcaaaggat atgatgaaat tcaccgacta cagcgtgaaa
     3601 accggacatc tgaccgccta ctggacgcca tctttcgctc aggatgtgtt agttaaagcc
     3661 agcgtcgggc agtatctggc aggggataaa ggcggcacgc tggagatcgc taaacgcttt
     3721 gacagcggcg tggtggtggg cggctatgcc acgatcacca acgtttcgaa agaggagtac
     3781 ggcgaagggg acttcaccaa aggcgtgtat gtctctgtac cgttggatct cttctcgtct
     3841 ggcccgacac gcagccgtgc ggcgattggc tggacgccgc tgacgcgtga cggtggtcag
     3901 caacttgggc gtaagttcca gttgtatgac atgaccagcg accgtagcgt caatttccgc
     3961 taagtcatgg gaaaggtgcc agttttcgca atcactggca cctttattct taatggcctg
     4021 ttgagccatc aggaacgcag ttcacccccg gtttgcgatc tggtgtgtaa aggcgaaaac
     4081 attctgtctt cctgtccgtc gatatcggtt gcaggtctgg ttgcctgagc gactgggcca
     4141 tcattgggct ggagacattg ctttgcggta ggtcggcttt atcagcagta ccgagcggac
     4201 cagcataagc aggaagagca gagactgata acatcaaagc agcaaaataa ggcttcattt
     4261 ttaccacctt tatcaggtta cgtttcattt gttccagagg aacattgtcg atttttcgcg
     4321 cattgctggt ggctgggaat cacctgaatg ggtgattttt gaattaccgg ctttggtgcg
     4381 gtttgtcttg ccggatgcgc cgccaggcgc ggcttatccg gcctacgggt aggtatatcc
     4441 ggctttggtg gaggcgcgct ccaaatccag gttgaacaaa acatacacaa aaaatataga
     4501 tctccgtcac atttttgcgt tatacaggaa gctcgccact gtgaaggagg tactgctatg
     4561 acgtcactct ctcgtccgcg cgtggagttt atctccacca ttttgcagac cgtactcaat
     4621 cttggcctgc tgtgcctcgg ccttattttg gttgtcttcc tcggcaaaga aacggtgcat
     4681 ctggctgatg tgctgttcgc gccagaacaa gccagcaaat atgagctggt agaaggactg
     4741 gtggtttact ttctctattt cgaatttatc gcgctgattg tgaagtactt tcagtccggt
     4801 tttcacttcc cgctacgtta ctttgtctat attgggatca ccgccattgt gcgcttgatc
     4861 atcgtcgatc ataaatcgcc actggatgtg ttgatctact cggctgcgat cctgttactg
     4921 gtggtcaccc tgtggctgtg taattcgaaa cggctgaagc gggagtaaaa agacagcacg
     4981 ccgaactggc gcggcgtgct ggacaggaag attacagcgt agcagtttgt tgtgttttct
     5041 tcgtttccgg ttcccagagg gcttccagct cctcaagggt tttacctttg gtttccggga
     5101 caaatttcca cataaacagt gctgccagaa cgcccataca accgtaaatc cagtaggaga
     5161 aaccgttgtg gaaatgggcc accagccagg agtttttgtc catcatcggg aaggtccagg
     5221 agacgaagta gttcgccagc cactgggccg ccaccgcgat tgccagcgct ttacctcgaa
     5281 tagcattcgg gaagatttcc gacagcagta cccagcatac cggaccccag gacatggcaa
     5341 aggcggcaac atagaacagc atcgacagta gcgccacaat acccggtgcc tgagtgtaaa
     5401 acgcggtacc gaggctaaac ataccgattg ccattccgag tgcgccgata atttgcagtg
     5461 gcttacgacc aaatttatcc accgtcataa ttgccagaac ggtgaaggtg aggttgataa
     5521 ctccgacaat aatggtctgc aacagcgcga tatccgtgct ggcccccagc gttttgaaca
     5581 cttccggcgc gtagtacagc accacattga tgccgacaaa ttgctggaag atggagagca
     5641 taacgccgat gacaatcacg cccacgccaa acatcagcag acgaccaccg gttttgcggc
     5701 catgatccag ggagtgttta atttcctgta ctgcctgagt tgcaagcgtg ttgcccataa
     5761 ttttgcgcag gataccttcc gcctgttctt gcttgccgcg tgacatcagc cagcgaggac
     5821 tttctggcac ggtatacagc agcattaaga acagcagtgc agggatacat tccgaggcaa
     5881 acatataacg ccagccgtca gtattcagcc agctggcatc accggaacgc gcaataaaat
     5941 agtttacgca gtaaactaaa agctgcccga aaataatcgc aaactggtta aatgaaacca
     6001 gtttcccgcg aatatgagca ggagcaagtt ccgcaatata cattggtgaa agcattgagg
     6061 ctaaaccaac gccaataccg ccaataatac gataaataac aaattccggg acataacctg
     6121 ccagataaac aggcactgtg ttgtccgggt ttatagaggt aaaaccaagt tctggccagg
     6181 cagaacctac accagaaata aaaaacagga cagcagcaat cttaagtgaa tcacgacgac
     6241 cgaagcggtt actgcaataa ccaccgaggg caccgccgat gatgcaacca atcagagcgc
     6301 tggccacgca aaaccctaac agggagttgg cagcggattc acttaagttt tgtggagcaa
     6361 caaagacggt attgagtgac tcaacagtac cggaaataac ggcggtgtcg tagccaaata
     6421 ataaaccacc taatgtagcg actaaggtaa tcgaaaatat ataactggaa ttatactggg
     6481 tattcattca gacctgcctt agaccattct gatcttttat cagtgcattg atgttggaat
     6541 tgtcaggcag ttaaggcaat tatcattttt tacaatgcgt ttacgtaatg tcttttctgt
     6601 gatcttaatt gtgataatta tccaaaattg ataaaaacaa tactattgcc gtgactcaga
     6661 gcacgaaaga gaattatcgt aagtgggaaa acaaataacg taaaaataat aagctctatg
     6721 atggaaatat taaccggcga acgattcaga ttgcagacga aagaaaaaaa ggcgctccgt
     6781 ggagcgccga ataacagtca caagttggga taacgtaagt tgagggtgca gcggcataac
     6841 attggcagaa caacatcttt aacctttcac accacctgcc gtcaggccgt tgaccagcca
     6901 gcgttgagcc agcaagaaga cgatggtgat cggtaatgca gacatcacgg cagcggcggc
     6961 aaagtcaccc cacaggtagt tttgcggatt gaggtattgc tgcatcccca cggccagggt
     7021 gtagctgttt acgtcacgca gtaacagcga cgcgaccgga acttcagtaa tggcagcgat
     7081 aaacgacagg atgaatacca ccgccagaat cggtactgac agcggcaaca ggacaaggcg
     7141 gaaggcctgc cacggtgtcg caccatccag cgcagccgct tcttccagcg aactgtcgat
     7201 ggtttcgaaa tagcctttga tggtccagac gtgcagcgca atcccaccca gatacgcgaa
     7261 aattacgccg ccgtgagtat tcaggccaat gaatggaatg tactcaccca gacgatcaaa
     7321 caacgcatac aacgcgacca gtgaaagtac tgccgggaac atctggaaaa tcagcattcc
     7381 tttcagcagc gtcgctttgc ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt
     7441 ggagagcgcc acaatgccaa tcgcggaaat cccggcgacc tttaccgagt tccacagcca
     7501 cagcagcacc gggaatggcg gtggcgtaat gcgaccatca gcctgttcca cgctaaaacc
     7561 taatgccagt ttccagtgat cccaggagat ttgctccggg atcaggctgc cggtcgcaaa
     7621 gttcccctgg cgcagtgaga tagcgacgac catcagcagc gggaacataa tcgccgcgat
     7681 aaaaagtagc agtagcaggt gagtaataaa caaacgtgct ttttgcgatt tcggttggac
     7741 cattgccatt tttgttatct cccttaatca aacttcattc gcgtggcttt caggttcact
     7801 atcgccagcg cacccaccag caggaagatc agcgtggcaa ttgctgctgc cagaccgaag
     7861 tcctgacccc cgccgccttc aaaagcgatg cggtaggtgt agttaacaag caggtcggta
     7921 taaccggctg gcgtggtcgt gccaagacga tccgggccgc cgttggttaa cagttgaatc
     7981 agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca tcagcggcgt cagcggttta
     8041 atcagcagcg gcagcgtaat cttaaagaag ttctggaacg gacctgcgcc atccattgct
     8101 gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc ccatgcagag gatcatcatg
     8161 tacggatagc ccagccaggt attgacgata atcagcatcg ttcgggcggt ggtcggatcg
     8221 ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca tcatgttgat ctcaccgaag
     8281 ctctggttaa acaacccttt gaaaatcaag attgaaatga acgatggcac cgcgtagggc
     8341 agaatcagca ggacgcgata gaccgctttg ccgcgcaacg cttcccactg caccagacac
     8401 gccagaacca tgccgactgc caccgttaaa aagacagtga tcagcgagaa caccacggtc
     8461 cagacgaaaa tggcgaggaa cggtttctga atgccttcgt cggtgaagac gcgagtaaag
     8521 tttttccagc cggtggtcac ggtgtaaccg gggcttagct tttcatcacc ccagttgccg
     8581 tcggcggtaa tggactggta aaagccaatt tggttattcg gacgatattt cacgccgctc
     8641 tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac
     8701 tggcgcaggg agctcatcat cactttgttg ccatccggca gaatggcggt aatgtcactc
     8761 agcgcctgac gattctgggt aatcacgcgt agatttgcgc gttcgccttc gggctgggcg
     8821 gtcgtttctt tcagttgcag tttttgctct ctgccaaatt taaaagcgtc ggagaggtaa
     8881 tttttgccgg tttcgccgtc gctgagcgcc agttgccact catcgcccgc cgggtaaaga
     8941 ccaaagttat aggttttgcc tgcttgccag gagcgatcta acaacacttc ctgtgcacgt
     9001 tcaaaagtca gctggttagt gctgctgtag ttggtgaagg caatggcgat ggtgcagacc
     9061 agagggaaga ggacgaataa tcccattcca gccattcccg ggtaaacata gcgccaggcg
     9121 taggctttac gattggcgaa aatatacagc cccgctgaac tcaatatcag cgtggtaatg
     9181 gcgaacaggt attccccttg tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg
     9241 agcagaccta acactgacca tttcagcgcg tcgctttgcc accaatgttt ctttttaatg
     9301 acatccatgg ggttcttcct cattccagga cggataaggc tttcatgcct tatccgacaa
     9361 caactgcctg atgcgacgct gacgcgtctt atcaggccta catacgtttc ggttttgtag
     9421 gccggacaag gcgttcacgc cgcatccggc atttcacagc attacttggt gatacgagtc
     9481 tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca
     9541 gtacgcacgg cataccagaa agcggacatc tgcgggatgt tcggcatgat ttcacctttc
     9601 tgggcgtttt ccatggtggc tgcaatacgt ggatctttcg ccaactcttc ctcgtaagac
     9661 ttcagcgcta cggcacccag cggtttgtct ttattaaccg cttccagacc ttcgtcagtc
     9721 agcagatagt tttcgaggaa ctctttcgcc agctctttgt tcggactggc ggcgttaata
     9781 ccggcgctca gcacgccaac gaacggttta gatggttgac ccttgaaggt cggcagtacc
     9841 gttacaccat aattcacttt gctggtgtcg atgttggacc atgcccacgg gccgttgata
     9901 gtcatcgctg tttcgccttt attaaaggca gcttctgcga tggagtaatc ggtgtctgca
     9961 ttcatgtgtt tgtttttaat caggtcaacc aggaaggtca gacccgcttt cgcgccagcg
    10021 ttatccacgc ccacgtcttt aatgtcgtac ttgccgtttt catacttgaa cgcataaccc
    10081 ccgtcagcag caatcagcgg ccaggtgaag tacggttctt gcaggttgaa catcagcgcg
    10141 ctcttacctt tcgctttcag ttctttatcc agcgccggga tctcttccca ggtttttggc
    10201 gggttcggta gcagatcttt gttataaatc agcgataacg cttcaacagc gatcgggtaa
    10261 gcaatcagct tgccgttgta acgtacggca tcccaggtaa acggatacag cttgtcctgg
    10321 aacgctttgt ccggggtgat ttcagccaac aggccagatt gagcgtagcc accaaagcgg
    10381 tcgtgcgccc agaagataat gtcagggcca tcgccagttg ccgcaacctg agggaatttc
    10441 tcttccagtt tatccggatg ctcaacggtg actttaattc cggtatcttt ctcgaatttc
    10501 ttaccgactt cagcgagacc gttatagcct ttatcgccgt taatccagat taccagttta
    10561 ccttcttcga ttttggcgag agccgaggcg gaaaacatca tcgtcgttaa tgcggataat
    10621 gcgaggatgc gtgcacctgt ttttattttc ataatctatg gtccttgttg gtgaagtgct
    10681 cgtgaaaaca cctaaacgga ctctagtttc tttatacggc aacctctttc catcctcctt
    10741 gcccctacgc cccaccgttg ctttgtgtga tctctgttac agaattggca gtaatgtgga
    10801 gatgcgcaca taaaatcgcc acgatttttg caagcaacat cacgaaattc cttacatgac
    10861 ctcggtttag ttcacagaag ccgcgttctc atcctcccgc ctcctccccc ataaaaaagc
    10921 tagggggtgg aggatttaag ccatctcctg atgacgcata gtcagcccat catgaatgtt
    10981 gctgtcgatg acaggttgtt acaaagggag aagggcatgg cgagcgtaca gctgcaaaat
    11041 gtaacgaaag cctggggcga ggtcgtggta tcgaaagata tcaatctcga tatccatgaa
    11101 ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta aatcgacttt actgcgcatg
    11161 attgccgggc ttgagacgat caccagcggc gacctgttca tcggtgagaa acggatgaat
    11221 gacactccgc cagcagaacg tggcgttggt atggtgtttc agtcctacgc gctctatccc
    11281 cacctgtcag tagcagaaaa catgtcattt ggcctgaaac tggcaggcgc aaaaaaagag
    11341 gtgattaacc aacgcgtcaa ccaggtggcg gaagtgctac aactggcgca tttgctggat
    11401 cgcaaaccga aagcgctctc cggtggtcag cgtcagcgtg tggcgattgg ccgtacgctg
    11461 gtggccgagc caagcgtatt tttgctcgat gaaccgctct ccaacctcga tgctgcactg
    11521 cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac gcctgggccg cacaatgatt
    11581 tacgtcaccc acgatcaggt cgaagcgatg acgctggccg acaaaatcgt ggtgctggac
    11641 gccggtcgcg tggcgcaggt tgggaaaccg ctggagctgt accactatcc ggcagaccgt
    11701 tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc tgccggtaaa agtgaccgcc
    11761 accgcaatcg atcaagtgca ggtggagctg ccgatgccaa atcgtcagca agtctggctg
    11821 ccagttgaaa gccgtgatgt ccaggttgga gccaatatgt cgctgggtat tcgcccggaa
    11881 catctactgc cgagtgatat cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag
    11941 caactcggca acgaaaccca aatccatatc cagatccctt ccattcgtca aaacctggtg
    12001 taccgccaga acgacgtggt gttggtagaa gaaggtgcca cattcgctat cggcctgccg
    12061 ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat gtcgtcgact gcataaggag
    12121 ccgggcgttt aagcacccca caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga
    12181 aaagcaatga ctcaggagat agaatgatga ttactctgcg caaacttcct ctggcggttg
    12241 ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt tgatttccac ggctatgcac
    12301 gttccggtat tggctggaca ggtagcggcg gtgaacaaca gtgtttccag actaccggtg
    12361 ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta tgctgaatta aaattgggtc
    12421 aggaagtgtg gaaagagggc gataagagct tctatttcga cactaacgtg gcctattccg
    12481 tcgcgcaaca gaatgactgg gaagctaccg acccggcctt ccgtgaagca aacgtgcagg
    12541 gtaaaaacct gatcgaatgg ctgccaggct ccaccatctg ggcaggtaag cgcttctacc
    12601 aacgtcatga cgttcatatg atcgacttct actactggga tatttctggt cctggtgccg
    12661 gtctggaaaa catcgatgtt ggcttcggta aactctctct ggcagcaacc cgctcctctg
    12721 aagcaggtgg ttcttcctct ttcgccagca acaatattta tgactatacc aacgaaaccg
    12781 cgaacgacgt tttcgatgtg cgtttagcgc agatggaagt caacccgggc ggcacattag
    12841 aactgggtgt cgactacggt cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg
    12901 catcgaaaga cggctggtta ttcactgctg aacatactca gagtgtcctg aagggcttta
    12961 acaagtttgt tgttcagtac gctactgact cgatgacctc gcagggtaaa ggtctgtcgc
    13021 agggttctgg cgttgcattt gataacgaaa aatttgccta caatatcaac aacaacggtc
    13081 acatgctgcg tatcctcgac cacggtgcga tctccatggg cgacaactgg gacatgatgt
    13141 acgtgggtat gtaccaggat atcaactggg ataacgacaa cggcaccaag tggtggaccg
    13201 tcggtattcg cccgatgtac aagtggacgc caatcatgag caccgtgatg gaaatcggct
    13261 acgacaacgt cgaatcccag cgcaccggcg acaagaacaa tcagtacaaa attaccctcg
    13321 cacaacaatg gcaggctggc gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa
    13381 cctacgccaa gtgggatgag aaatggggtt acgactacaa cggcgatagc aaggttaacc
    13441 cgaactacgg caaagccgtt cctgctgatt tcaacggcgg cagcttcggt cgtggcgaca
    13501 gcgacgagtg gaccttcggt gcccagatgg aaatctggtg gtaatagcaa aacctgggcc
    13561 ggataaggcg tttacgccgc atccggcata aaacaggttg tcattatctg aaaggggcga
    13621 aagcccctct gattatcggg tttagcgcgc tattgcctgg ctaccgctga gctccagatt
    13681 ttgaggtgaa aacaatgaaa atgaataaaa gtctcatcgc cctctgttta tcagcagggt
    13741 tactggcaag cgcgcctgga atcagccttg ccgatgttaa ctacgtaccg caaaacacca
    13801 gcgacgcgcc agccattcca tctgccgcgc tgcaacaact cacctggaca ccggtcgatc
    13861 aatctaaaac ccagaccacc caactggcga ccggcggcca acaactgaac gttcccggca
    13921 tcagtggtcc ggttgctgcg tacagcgtcc cggcaaacat tggcgaactg accctgacgc
    13981 tgaccagcga agtgaacaaa caaaccagcg tttttgcgcc gaacgtgctg attcttgatc
    14041 agaacatgac cccatcagcc ttcttcccca gcagttattt cacctaccag gaaccaggcg
    14101 tgatgagtgc agatcggctg gaaggcgtta tgcgcctgac accggcgttg gggcagcaaa
    14161 aactttatgt tctggtcttt accacggaaa aagatctcca gcagacgacc caactgctcg
    14221 acccggctaa agcctatgcc aaaggcgttg gtaactcgat cccggatatt cccgatccgg
    14281 ttgctcgtca taccaccgat ggcttactga aactgaaagt gaaaacgaac tccagctcca
    14341 gcgtgttggt cgggccttta tttggttctt ccgctccagc tccggttacg gtaggcaaca
    14401 cggcggcacc agctgtggct gcacccgctc cggtgccggt gaagaaaagc gagccgatgc
    14461 tcaacgacac ggaaagttat tttaataccg cgatcaaaaa cgctgtcgcg aaaggtgatg
    14521 ttgataaggc gttaaaactg cttgatgaag ctgaacgcct gggatcgaca tctgcccgtt
    14581 ccacctttat cagcagtgta aaaggcaagg ggtaattacg ccccacagtg ctgattttgc
    14641 aacaactggt gcgtctcctg gcgcaccttt ttttatgctt cattccaggg atatgagcga
    14701 ttttttatag taactcactt cttcttcact aagaatatcc attatctcaa tgtattatca
    14761 gagattcttt tcctttcgcc ggtagtgtct ggacgctcag gctacttttt ccagttattt
    14821 catttctgta atgcagaggt tttatgttag gtcatatctc aaagtttgac ggcaataact
    14881 ctttgataaa acatggtgtg gtgcaaggaa ataatatagt agattttgat ttactacgta
    14941 attttaatgg ggggccag
//
LOCUS       DAZFDC010000117        14658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFDC010000117 DAZFDC010000000
VERSION     DAZFDC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.117"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(249..764)
                     /gene="pgpA"
                     /locus_tag="ACHJ48_003698"
     CDS             complement(249..764)
                     /gene="pgpA"
                     /locus_tag="ACHJ48_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO4709173.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(742..1719)
                     /gene="thiL"
                     /locus_tag="ACHJ48_003699"
     CDS             complement(742..1719)
                     /gene="thiL"
                     /locus_tag="ACHJ48_003699"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO4709174.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1797..2216)
                     /gene="nusB"
                     /locus_tag="ACHJ48_003700"
     CDS             complement(1797..2216)
                     /gene="nusB"
                     /locus_tag="ACHJ48_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO4709175.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2236..2706)
                     /gene="ribE"
                     /locus_tag="ACHJ48_003701"
     CDS             complement(2236..2706)
                     /gene="ribE"
                     /locus_tag="ACHJ48_003701"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO4709176.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2795..3898)
                     /gene="ribD"
                     /locus_tag="ACHJ48_003702"
     CDS             complement(2795..3898)
                     /gene="ribD"
                     /locus_tag="ACHJ48_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO4709177.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDVQTQAL
                     YPQHHLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3902..4351)
                     /gene="nrdR"
                     /locus_tag="ACHJ48_003703"
     CDS             complement(3902..4351)
                     /gene="nrdR"
                     /locus_tag="ACHJ48_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO4709178.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4502..5041
                     /locus_tag="ACHJ48_003704"
     CDS             4502..5041
                     /locus_tag="ACHJ48_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO4709179.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5340..6224
                     /gene="tsx"
                     /locus_tag="ACHJ48_003705"
     CDS             5340..6224
                     /gene="tsx"
                     /locus_tag="ACHJ48_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO4709180.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6401..6748)
                     /gene="yajD"
                     /locus_tag="ACHJ48_003706"
     CDS             complement(6401..6748)
                     /gene="yajD"
                     /locus_tag="ACHJ48_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO4709181.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6836..7117)
                     /locus_tag="ACHJ48_003707"
     CDS             complement(6836..7117)
                     /locus_tag="ACHJ48_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4709182.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGDEQADRYTDSLGTFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(7110..7352)
                     /locus_tag="ACHJ48_003708"
     CDS             complement(7110..7352)
                     /locus_tag="ACHJ48_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO4709183.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(7504..8475)
                     /gene="secF"
                     /locus_tag="ACHJ48_003709"
     CDS             complement(7504..8475)
                     /gene="secF"
                     /locus_tag="ACHJ48_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO4709184.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(8486..10333)
                     /gene="secD"
                     /locus_tag="ACHJ48_003710"
     CDS             complement(8486..10333)
                     /gene="secD"
                     /locus_tag="ACHJ48_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO4709185.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(10361..10693)
                     /gene="yajC"
                     /locus_tag="ACHJ48_003711"
     CDS             complement(10361..10693)
                     /gene="yajC"
                     /locus_tag="ACHJ48_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO4709186.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10716..11843)
                     /gene="tgt"
                     /locus_tag="ACHJ48_003712"
     CDS             complement(10716..11843)
                     /gene="tgt"
                     /locus_tag="ACHJ48_003712"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO4709187.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11898..12968)
                     /gene="queA"
                     /locus_tag="ACHJ48_003713"
     CDS             complement(11898..12968)
                     /gene="queA"
                     /locus_tag="ACHJ48_003713"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO4709188.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     SGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            13061..13642
                     /gene="acpH"
                     /locus_tag="ACHJ48_003714"
     CDS             13061..13642
                     /gene="acpH"
                     /locus_tag="ACHJ48_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO4709189.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13663..>14658)
                     /locus_tag="ACHJ48_003715"
     CDS             complement(13663..>14658)
                     /locus_tag="ACHJ48_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709190.1"
                     /translation="DSSTHEYKIDHQQLKNHIRQLEEWALLEKFYDNNAYYSKRTKQL
                     EKKIADAGFITRKVKAENFNVKEFKDDIASMLCEQPPMRTHQISSSGYVSADKLHKLY
                     KTNLEDAQRDLKTTPVDIDGVSLKSITREKIAEEIAKKTAEIIAKQNAKSIEEKNIEK
                     NKKQTNSEDNFVLKFSPEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLHNV
                     ARNGSPRYAGLLRQIASKSAILQDFDQQLFWRTFTGGVLHRHISVKSEPEKTDVWRFS
                     SQFDFPHINENGELSRTVLMFEGVAESNFKDIANHSSECFSYRCYAAPLDANAKK"
BASE COUNT         3642 a         3900 c         3486 g         3630 t
ORIGIN      
        1 agaataattt tgatgatatt tttcgtcaac agtcagagaa tatgagcaat tattatgagt
       61 tcgtcgactg acgatatttc agtgtcattg aattatgagt gacaaagata caccttgaaa
      121 tatcgccgca tagtatattc ggcgatgtcg ggttttactt taacagagtg ccccaaaatt
      181 ggggcactgt tcatttataa atcaaaccgg gtggtctgga atcgcctaca cctcccggtc
      241 aatggcattt aaagaatacc cagcggccag tgatgaccga taaagtacag gatgcctgcg
      301 gaaatcaccc cggcgacaat atcatcgatc atgatcccca tgccgccatg tacattgcga
      361 tcaaaccagc ggatcggcca cggcttccac atatccagaa tacggaaaat cacaaacccg
      421 gcggcgaccc actgccagtc attggtcggc agcgccatca gggtgatcca catgccaata
      481 aactcatccc atacgatgct accgtgatcg tgcaccccca tgtctttcgc cgtttgatga
      541 caaagataga cgccgataca gatccccaac atcaccacca gcgagtagag ctgccagggc
      601 aaaaaggtca tcagatacca gaacggaatc gccgccagcg atcccatcgt gccaggaacg
      661 attgggctta atccacttcc gaatccgaca gcaagcagat gccacggatt actcatcttc
      721 aggcgacttt tcgcgacatc tttatggcgt ggcaaaatgg tcatatcctt tccagtctaa
      781 cgtgacaggt tcgccgtcac gaataaaaca aagcccttcg atatcggcgg tcatttgccc
      841 gatacaggta aacggtacgc ccagatgacc gagagccaca tccagcgcgc cacggttcag
      901 ttccggcacc gtgaaacaca actcgtaatc ttcaccgcca gagagcgccc agcgcagcgc
      961 ctgttccggt acaacatggc gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat
     1021 acgtgcgccg cagtcgctgg ctttcacgat atgcccgaga tcggaaatca gaccgtcaga
     1081 gagatcgata gctgaatttg ccagatcgcg cagtgcctgc ccctgtaaaa tacgcggcga
     1141 tggacggaga tgacgtttga tcaagtagtc cgcatcttta gcatcggcaa cctgcaaacg
     1201 gttttgcaaa atcgccagcc cggcggcgct atcgcccggt gtaccggtca catagatcca
     1261 gtcacccggt ttcgccccag agcgcgttaa ggctcgtccc atcggaacaa agccgtggat
     1321 acccaacgtc attgataatg gcccacgcgt ggtatcgccg ccaatgagtt gcatatcgta
     1381 ataattgaga agatcaaaca aactgtcgct gaaggactca agccacgctt cgtctacgtc
     1441 cggtaaggtt aatgccagcg tcagccaggc cggatcggcc cccatcgctg ccagatcgct
     1501 taggttcacc gccagtgctt tataagccag atcagcagga tcgatatcag ggaggaaatg
     1561 gttgcccgcc accagcgtat cagtgctgat cgccagggtc tgtttctcgg ggatattgag
     1621 aagagcgcaa tcgtcgccga tgcccagttc gacatcaaga cgagaacttc ttacacggtc
     1681 aaaataacgg gcaatcaggg agaactcgcc acatgccata cgttatgcct cagcaggtaa
     1741 aaaagaaaag gccggcgatc gcggaacagt cttccgtgaa tctaccggcc tggatattac
     1801 tttttgttag ggcgaatcac aggtgctgct ttatcgagta cgccgttgac gaacttatgg
     1861 ctgtcttctg cgccgaacga tttcgccagt tcgatcgctt cgttaatggc cactttgtat
     1921 ggcacatcgc tacgtttaga cagttcgtac agcgcaatgc gcagtactgc tttttctacc
     1981 tgtcccagtt cttccagcag gcgggacagg tatggcttca tcagtccgtc gaggtatgcg
     2041 gtattagtcg ccaccccggc cagcagctca cggaagtaca ggacgtcaac gtcttttaca
     2101 tcctgttcag ccaggaactg gtattcaaca tcagcgatgt cgttctggga caactgccag
     2161 gagtagagcg cctggacggc acactcacga gcgcggcgac gagcagcagg tttcacggat
     2221 ttccccttac taatttcagg ccttgatggc tttcaataca ttaatcattt caagcgcggt
     2281 cagtgcagct tctgcacctt tgttgccagc tttggtgcca gcacgttcga tcgcttgttc
     2341 aatgctttca gtggtcagaa ccccaaaagc aaccggaatt tcgctgtcct gggcaacatg
     2401 cgccaggccg ttgcttgcac caccagcgac atattcaaaa tgggcagtgc caccacgaat
     2461 aaccgtaccc agcgcaatca ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc
     2521 cagcggcagc tcataggcac caggcaccca aacaacggta atgttttcat cttttacctg
     2581 accgatacgt ttcagtgcat caattgcacc ttccagcagg ctgtcattga taaagttgtt
     2641 gaaacgcgcg atggtgatgg cgacgcgagc gtccggggta gcaacgttag cttcaataat
     2701 gttcattttc ttccttcggg ttcgagtatg gccccgcagg ggggcggatt ttagcataat
     2761 atttcgtgcg ctgcttccct ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa
     2821 catccgggcc tacatgacgt atctctttga atttaaattg gggggcgtca gctaatttct
     2881 caagccctgg cagtgtgcat aatccacggg cgtcgctgcc taatagttta ggtgcgatat
     2941 agacaatcag ctcatcgact aaccccgcct gcagcaatgc gccagcgagc gttggccccg
     3001 cttccaccca gatgctgtta atctgctgtt tacccagttg catcatcagt acaaccagat
     3061 ccagatgacc tttatgctct ggaatcagca aggtacgcac cgtttccggc cactcacgag
     3121 aatcttcctg cgtacgcgcg aaccaggttt cgccgggctg ctgcacaatg tgatgttccg
     3181 gcgtcacgcg attttggcta tcaatcacaa tacgtaccgg ctggcggaga tgatgttgcg
     3241 ggtagagcgc ctgagtttgt acatccagtt cagaccaacg caccgttaag gcaggatcat
     3301 ccgccagcac cgtagcgctg ctggttaaaa tggcatgact ttgtgcgcgc agtcgttgta
     3361 cgtcgcgccg cgcctgaggc gaagtgatcc actggctttc gccgctcgcc atcgccgtgc
     3421 ggccatcaag cgatgcgcca agttttaact gaatataagg aaagccggtg cgcatccgct
     3481 tgagaaagcc tttattcaat tgctcggctt cactcatcat caggccgtgg ctgacgtcaa
     3541 tgccagcctg ttgcagacgg taaagtccac gcccagcgac ctgcgggttc gggtcctgca
     3601 tcgcagcgac cacgcgcgct acgccagcgg cgatgagtgc gtcacagcac ggtggcgtac
     3661 gaccatgatg gctacagggt tcgagtgtga cataggcggt cgcacctttg gctttttcac
     3721 ccgccatacg caacgcgtgt acttcggcat gtggttcacc cgctcgttgg tggtaacctt
     3781 caccgacaat ttcgccatct ttgacaatga cgcacccgac attcgggttg gggtgggtgg
     3841 taaaacgtcc tcgttgcgcc agctttagcg cccgcgccat gtaatactcg tcctgcacgg
     3901 cttagtcctc caggcgcgcg atctcttcgc caaattcttt gatatcttcg aaactgcggt
     3961 agacagaggc aaaacggata taggcgactt tatcgagctt tttcaattgc tccatcacca
     4021 gattgccaat catcttgctc ggcacttcgc gctcaccggt ggcgcgcagc tgcgatttaa
     4081 tatgattgat tgccatttcg acgtcatcgg aactcaccgg acgtttttcc agcgcccgca
     4141 gcattccgct acgtaatttt tcttcattaa acggttcacg cacgtcgttg ctttttacaa
     4201 cacgcggcat aaccagctcc gccacttcaa aggtggtgaa acgttcatta cacaccagac
     4261 actgccgacg gcggcgtacg gatgaaccct cgcccacgag acgagagtca attaccttag
     4321 tgtccacggc gaaacagaat gggcaatgca tacggcgtcc tgaccaggtg gttaacggaa
     4381 atctatttta ccctgaactg acgatacaac aaaggcgcaa cgcttttgag ataaaggatc
     4441 gatgctttcg ctgcttttac cctctaccat taggctaatt gcgacgagaa agggacgacc
     4501 tatgaatatt aacgtttttc gactgatcct gctgggaagc ctattcagcc ttagcgcctg
     4561 tgtgcagcaa agtgaagtgc gacagatgaa acacagcgtc agcacgctga accaggagat
     4621 gacgcaactc aatcaagaaa cagtcaaaat cactcagcaa aacaggctga atgcaaaatc
     4681 cagcagtggg gtttaccttc tgcctggagc gaaaacaccg gcaagactgg aaagccagat
     4741 cggtacttta cgtatgtcgc tggtgaatat tacgcctgat acagatggca ccaccctgac
     4801 actacgtatt cagggtgagt cgaatgatcc cttgcccgca ttcagcggaa ctgttgaata
     4861 tgggcaaatt cagggaacaa tagacaactt tcaggaaatc aatgtacaga atcaattgat
     4921 taatgcccct gccagcgtcc tcgcccccag cgatgttgat attccgttac agttaaaggg
     4981 gatttctgtg gatcagttag gctttgtacg tatccacgac attcaacctg ttatgcagta
     5041 aacttatagc ggggcgattt tgtgcgtccc gcaacatcct tccccatcat tttgttactc
     5101 cgcttacata acccagattg atagtaaaag tttgcaacaa gggcgaaagt cagtacaatc
     5161 cccgcccgaa tgtgtgtaaa cgtgaacgca atcgattacg taaatgatag aactgtgaaa
     5221 cgaaacatat ttttgtgagc aatgattttt ataataggct cctctgtata cgaaatattt
     5281 agaaacgcaa tttgcgcctt tttcactccc gcaagggatt ttcaaacagt ggcatacata
     5341 tgaaaaaaac attactggca gccggtgcgg tactggcgct ctcttcgtct tttactgtca
     5401 acgcagctga aaacgacaaa ccgcagtatc tttccgactg gtggcaccag agcgttaacg
     5461 ttgtcggaag ctatcacacc cgtttcggac cgcagatccg caacgatacc taccttgagt
     5521 acgaagcatt cgctaaaaaa gactggttcg acttctatgg ttatgcggat gcgccggtat
     5581 tcttcggcgg taactccgat gcaaaaggta tctggaacca cggttctcct ctgtttatgg
     5641 aaatcgaacc acgtttctcc atcgacaagc tgaccaatac tgaccttagc ttcggtccgt
     5701 tcaaagagtg gtacttcgcg aacaactaca tttacgacat gggtcgtaat aaagatggtc
     5761 gccagagcac ctggtacatg ggtctgggta ccgatatcga cactggcctg ccgatgagcc
     5821 tgtccatgaa cgtctatgcg aaataccagt ggcagaacta tggcgcagcg aacgaaaacg
     5881 agtgggacgg ttaccgtttc aaaattaaat actttgtgcc gattaccgat ctgtggggcg
     5941 gtcagctgag ctacatcggc ttcaccaact tcgactgggg ttccgattta ggggatgaca
     6001 gcggtaacgc aatcaacggt attaagaccc gtactaataa ctctatcgct tccagccata
     6061 ttctggctct gaactacgat cactggcact actctgtcgt agctcgttac tggcacgacg
     6121 gtggtcagtg gaacgacgat gcagaactga acttcggcaa cggcaacttc aacgttcgct
     6181 ctaccggctg gggtggttac ctggtagtag gttacaactt ctgattatga aaatgccggg
     6241 atttattccc ggcatttcag attgttaaca acgtacgctt tgttcatgcc ggatgcggcg
     6301 taaacgcttt gtccggccca caaaagcacg taaattcaat atattgctga aataacgtag
     6361 gcctgataag cgtagcgcat caggcaattt tacgttttaa tcacttcttc ttgttcatca
     6421 tcgctttcag gtcagcgaat gggttgtact tcgcttcacc gacatctttc tgcgcatctt
     6481 cccctgcgat aacggtcgta ccatactgat ccgcttcggt atatttcgaa tgctcatgat
     6541 cgtggcaata gagacaaagc aattcccagt tactgccatc ttccgggtta ttggtatggt
     6601 cgtgatcaat gtggtgaacg gtaagttcac gcaggttgga ataaacaaac tcgcgggaac
     6661 agcgaccgca gacccacgga tagattttta atgctttttc gcgatagccg ctttctaacc
     6721 gcgcatagtt ttttgggatg atagccatga aaagttacct ggatgaaatg tagggttacg
     6781 cgcagtttcc caagatgcgg ggattttcgc aatcctgagt caagaaattc tcacattacc
     6841 agtgacggcg aggttccatc aactgatgaa gaatgcgcgc aatgagaatg tcatcgactc
     6901 ttataacgta ataaacagtg tgaaacctaa atgcgatctt ctttacctct ggaatcgcat
     6961 catagtcttg ccctatctcg ggcatttgag agagtgtatc cagaaaagtg ccgagagagt
     7021 cggtatagcg atccgcctgc tcatcaccaa actttcccag cgtgtagtca tagatccctc
     7081 tgaaatcttc aacagctttt ccgctaagct tatacacgat gtttttgctt cctttctgct
     7141 gcgatatcac gaagcgtcat cgaactttca ccgctctcaa gaccttcaat aacagcattt
     7201 ctgacagctt catccctgga ttcttgttgt tccaatagac gaagtgccga acgcattact
     7261 tcactggccg agccataacg accagactgg accatccggg taataaaact atctaattgt
     7321 tcgccaacgc ttactgaggt aattttgcgc ataagcgatc tccacgtatt aactattaat
     7381 acaaagtata tgtcagaacg ctttctacaa ccaaaccgta ttcagttgcc tgtgaaacag
     7441 gcaaaaaaaa accgcagcgc aaatacgcca cggttttttt aacagaacgg atttaaaaca
     7501 ccattacggc agaattgacg gctgatccgc cccttctttt tccactttct gctgcaacat
     7561 gtgttcgcgc ttcataccca gtttcagagc caacgcagat gccacataga tggaagatgc
     7621 agtaccgatg gaaacaccga taagcatggt cagcgagaag ccttccagta ccggaccacc
     7681 gaagagatac agcatcagga taaccatcaa ggtagtaccg gatgtgatca aggtacggtg
     7741 cagcgtctgg gtcaaggaca cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg
     7801 gaagttttca cgaattcggt ccgataccac gatactgtcg ttaagcgagt aaccgataac
     7861 cgacatcaac gatgccacaa tggtcaggtc aatctcgata tggaataacg acaaaatacc
     7921 cagcgtaatg ataacgtcgt gcgccagcgc aataaccacc cctgccgcca gtcgccactc
     7981 aaagcggaaa cctacgtaca cgaggataga cagcagcgct gccatcaacg ccatcgcacc
     8041 ggtttgcgca aggtctgccc ccacgctcgg accgacgaac tcaatacgct tcactgctgc
     8101 attctgattg gtggattcgt taatcacctt cagaacctgg ctgcccagca cctgaccgcc
     8161 ggtttcgcct tcagcaggcg gcatacggac catgatgtcg tggctgctac caaagttttg
     8221 cagcatcggc tcttcaaaac cggctttttg caatgcatca cgcattacgt caatttcagc
     8281 cggtttttcg agcgtaattt cgataaccgt accaccggtg aaatccagcc cccagttaaa
     8341 gccgcgcacg cccataataa cgatagcagc gattaacagc agaccagaga tgccaaaagc
     8401 ccagtagtcc cagcgcataa agtcatagac tttacggccg tggtttagtt gttcaacagt
     8461 atattcctgt gccacatcgc actcctcaga ttgacagctt cttgacgcgc ttgccgccat
     8521 atagcaggtt tacgatggca cgcgtaccga caatcgcggt aaacatcgac gtcgccacac
     8581 cgataccggt agtaatcgcg aaccctttaa ttgccccggt acccactgcg tacaggatga
     8641 tgactttaat cagcgtggtg atgttcgcat cgaagataga actgaatgcg ccacgataac
     8701 cttcatcaat tgcctgttga acagtacgtc cgttgctcaa ctcttcttta atacgttcgt
     8761 tgatcagtac gttcgcatcg accgctaccg caagggttaa gacgataccc gcgatacctg
     8821 gcatactcag tgttgcgcct ggcaacagcg acataatacc gacgatcaag atcaagttgg
     8881 caatcagagc actggtcgca atcagaccaa acttcttata gaagatgatc atgaacagaa
     8941 tagaaaccag cagaccggca aggcaagcct ccagcccctg ctcaatgttc tgcataccca
     9001 gggtcgggcc aattgtacgt tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac
     9061 gcagcagcag tgacagctga cgggcttcgt tcgggttgtt gatgccggtg atacggaagc
     9121 tgttacccag acgagactgg atgttggcga tgttaatcac ctcttcctgt ttcaccagaa
     9181 ccgcacgacc atttgcatct ttcttaccgc tgtctttgta ctccacaaac agggttgcca
     9241 tcggtttgcc gatgttgtcc ttggtgaagt tagacataat gttgccacca gcgctatcga
     9301 gcgagatgtt aacctgcggc tggttgtatt cgtcctggct ggaagtggag tcggtgatat
     9361 ggtcaccggt aagaattacg cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt
     9421 tcacttcaga gtcgcccggt acgcgaccgg atgccgcagc ggcctgatca acgttggtgt
     9481 ttaccagacg gaattccagc gttgccgtcg cacccagaat ctctttcgca cgcgcagtgt
     9541 cctgaatacc tggcagttca acaacgatac ggtcagcacc ctgacgctga accaccggct
     9601 ccgccacgcc aagttggttt acacggttac gcaggatatt gatgttctgc tgcaccgcat
     9661 attcacgcgc ttcactcaga cgagcatcgc tcattaccgc acgcagctgg ttgctgccct
     9721 gactgctaat caccaggtcc ggatgacgct tgctcagata cgcaatggct tcatcacgag
     9781 ctttagcatc gcggaaagtg atgctcaggc cgtagttatt ttctttacga acggtggtat
     9841 acgggatgcc tttttcgcgc aggtcgctgc gcaggctatc gatattttgt tcctggagtt
     9901 tgccaagcgc ggtgtccata tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa
     9961 ggccgagctt catcggctca gcgtgaatag ctgccagcca gcgcggcgtt gccggggcaa
    10021 ggttaagcgc cacgacgtat ttgtcaccca taacgcccat taatgcttca cgagcgcgca
    10081 gctgggtgtc agtggagtcg aagcgcgcaa gaatagcgcc ctcttccagt gccacagact
    10141 tagcagttat tttttcttct tgtaacgttt tctggacctg gatcagcgtt tgctcactgg
    10201 cggcgactcc gcgcgcacca gtgatctgaa cagccggatc ctcaccaaac aggttgggaa
    10261 gcgcatacag cagaccgatg acaatcacca cgatcagcat gacgtacttc cacaaaggat
    10321 aacggtttaa cacggcaatt cccttaggga aaaattttaa ttacagcgcc ttcatggtgc
    10381 ctttcggcag gacggcagct acgaagtcac gtttaataac tacttcagtg gtgtcattca
    10441 gcgcgatagc aatgtagccg ttttccgcta ctttggttac gcgaccaacc aggccaccgt
    10501 tcgtcagaac ttcatcacct ttggcaatgg agtccatcag ctttttgtgt tctttggtgc
    10561 gcttctgctg tggacgcagg atcatgaaat agaaaatcag accgaatacc accagcatca
    10621 aaatcaaaga catcgggcta ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa
    10681 taaaaaagct cattaaattt ccctcattat taatattaat caacgttcaa aggtggtact
    10741 tctcgcccct gacgctggta aaaatcagtt acgaagctct ctaatttacc ctcttcaata
    10801 gccttgcgta aacccgccat caaacgctgg tagtaacgaa ggttatgaat ggtattgagt
    10861 cgcgcgccta atatttcgtt gcaacggtca agatgatgca agtaagcgcg tgaataattg
    10921 cgacaggtgt agcaatcaca ctcaggatcg agtgggccag tatcgctctt atacttcgca
    10981 ttgcggattt tcaccacgcc atcggtcacg aacaaatgac catttcgggc gttgcgggtt
    11041 ggcattacgc agtcaaacat atcgatacca cgacgtacgc cttcaaccag gtcttctggt
    11101 ttaccaacgc ccatcaggta acgcggtttg tctgccggaa tttgcgggca tacatgctcc
    11161 agaatgcggt gcatatctgc tttcggctca cccacagcca gaccgccgac agcgtagcca
    11221 tcaaaaccga tatctaccag acctttaaca gaaatatcac gtaaatcttc gtaaacgctg
    11281 ccctgaatga taccaaacag cgcatttttg tttccgagac tgtcaaaacg ctcacggcta
    11341 cgcttcgccc aacgcagaga catctccatg gagcgttttg cgtaatccca gtcagcagga
    11401 tacggcgtac actcatcaaa gatcatgacg atatccgaac caagatcgta ctgaatctcc
    11461 atcgactttt ccgggtcgag gaaaatcggg tcgccgttga tcgggttacg gaagtgcacg
    11521 ccctgttcgg tgattttacg aatatcgcca aggctgaaga cttggaagcc gccggagtcg
    11581 gtgaggatcg gtcccttcca ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt
    11641 tcctggcccg ggcgcagcca caggtggaag gtgttgccga ggataatttg cgcgccagtg
    11701 gcttcaactt cttccggcgt catccctttt acggtgccgt aggtgccaac aggcataaaa
    11761 caaggcgttt ccactacgcc acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg
    11821 gtggtgtcca gttcaaattt cattttttct ccactacgtc agaaaaacag tccaacgttt
    11881 aaaccagcgc cgcggaatta ctccccgacg cgctcattaa ttgcctgcgg attgtacgtg
    11941 ataaacatcg catcaccgta actaaaaaag cgatatttct cttctaccgc cgctttatag
    12001 gcattcattg tgtgttgata accggcaaag gccgaaacca gcataatcag cgtcgattcc
    12061 ggaaggtgga agtttgtcac cagcgcatcg accactttgt actggaagcc cggatagata
    12121 aagatttggg tatcgtcaaa gaacggttcg atgagatcgt ttttcgccgc ctgagccgcg
    12181 ctttccagcg aacgtactga ggtggtgcca accgcaatca cccggttacc gcgcgctttc
    12241 gccgccagta ccgcgtctac cacatcctgc ggtacttcag cgtattccga gtgcatgatg
    12301 tgatcttcaa tggtgtcgac gcgtaccggc tggaaggtgc ccgcaccaac gtgcagggtg
    12361 acgaacgcca tctccacgcc tttggcgcgt aatttttcca gcaaaggttc gtcaaaatgc
    12421 agaccagcgg tcggggctgc aaccgcgccc ggtttttcgc tataaacggt ttgataaagt
    12481 tcgcggtcag cgtcttcgtc cggacggtcg atatacggcg gcagcggcat atggccgatg
    12541 ctgttgagaa tatccagcac cgagcgttca tcattaaatt cgacttcaaa cagtgcgccg
    12601 tggcgcgcgg tcattgttgc gttaatactt tcgtcatcgc ccagcagcag ttctgcgcca
    12661 gattttggcg ctttcgaggc gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgt
    12721 tcaaccagca cttcaatctt gccgccgctg gctttacgcc caaacaggcg cgccgggatc
    12781 acccgggtat tattaaaaac cagaagatcg ccggggttga gcttatcgag taaatcggtg
    12841 aaagtaccgt gcgtcagcgc gcccgtcggc ccgtccagcg acagtaaacg acagctactg
    12901 cgttcaggca tcggatagtg ggcaatcagg gattcgggca attcaaagga gaaatcggta
    12961 acgcgcatga cactgactca gactaaaata agaggcgggt agtctagtgc cggggcgctc
    13021 tccctgcaac ctttaccccc ctttggatag aatataatcg atgaattttt tagctcacct
    13081 gcatttagcc catctcgcgg aaagctcgct ttccggcaat ttgctggctg atttcgtacg
    13141 cggaaatcct gaagaaagtt ttccgcccga cgtcgtggct ggcattcata tgcatcgacg
    13201 tatcgacgta ttgactgaca atctgccgga agtccgcgaa gcacgggagt ggtttcgtag
    13261 tgaaactcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact ttctttcccg
    13321 tcactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtttgtt atgcccgcga
    13381 gcaagtgatg acaattttgc cggactcacc gccacgcttt atcaacctga acaactactt
    13441 atggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga acgtgttaaa
    13501 cggcatggca agccgtcgcc cacgtctgga tgccctgcgt gactcctggt acgatttaga
    13561 cgcccattat gccgccctcg aaacccgctt ctggcagttt tatccacgga tgatggcgca
    13621 ggcgtcacgc aaggcgctct gaaacttcaa actccggcgt cactattttt tagcattcgc
    13681 atccagcggg gcggcataac aacggtaaga aaaacattcg ctggaatgat tggcgatatc
    13741 tttaaagttg ctttcagcaa ccccctcgaa catgagtacc gttctggaca actctccgtt
    13801 ttcatttata tgtggaaaat caaattggct actgaatcgc catacatctg tcttttctgg
    13861 ctcgctcttc acagagatat gtcgatggag gacccctcct gtaaacgtgc gccagaacaa
    13921 ttgttgatca aaatcttgta atatcgcaga cttggatgct atttgacgta gtaaacctgc
    13981 ataccgtgga ctgccattac gagcaacatt atgcaaaccc tgactaaaag catcaaacaa
    14041 acaatagatg tgatcactag tgaaaaaagg tcttaatttt ttttgttctt catcgctgta
    14101 atccaggagc atctcattga tttcttccgg tgagaatttt aagacaaaat tatcctcaga
    14161 atttgtttgt ttcttgtttt tttcaatatt cttctcctca atgctcttcg cattttgttt
    14221 cgcaattatt tccgcagttt ttttcgcaat ttcttccgca attttttccc gagttattga
    14281 ttttagacta acaccatcga tgtcaaccgg tgttgttttc agatctctct gcgcgtcttc
    14341 caaatttgtc ttatagagtt tgtgcagttt atcggcagat acgtatccgg atgaagaaat
    14401 ctggtgtgta cgcatggggg gctgttcaca aagcatcgag gctatatcgt ctttaaactc
    14461 ttttacatta aagttctcgg ctttaacctt ccttgttata aaccccgcat cggcaatttt
    14521 cttctctaat tgttttgtac gtttagaata ataagcgttg ttatcataaa acttttcaag
    14581 aagggcccac tcctcaagtt gccttatgtg atttttcaac tgttgatgat ctattttata
    14641 ttcatgtgta ctagaatc
//
LOCUS       DAZFDC010000118        14639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFDC010000118 DAZFDC010000000
VERSION     DAZFDC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.118"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..210
                     /locus_tag="ACHJ48_003716"
     CDS             <1..210
                     /locus_tag="ACHJ48_003716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709191.1"
                     /translation="LSIYHVICSVTSKINIKNSAKFNIRSTSRLTYDIISQLHLPQNG
                     YFMEHSNFLVAIIKLILRSLAIIPG"
     gene            1184..1900
                     /gene="nanC"
                     /locus_tag="ACHJ48_003717"
     CDS             1184..1900
                     /gene="nanC"
                     /locus_tag="ACHJ48_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO4709192.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1920..3026
                     /gene="nanM"
                     /locus_tag="ACHJ48_003718"
     CDS             1920..3026
                     /gene="nanM"
                     /locus_tag="ACHJ48_003718"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO4709193.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLETQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLISHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            3091..4071
                     /gene="nanS"
                     /locus_tag="ACHJ48_003719"
     CDS             3091..4071
                     /gene="nanS"
                     /locus_tag="ACHJ48_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4709194.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQSYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNILANIIFVDFQQQGARGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWRER"
     gene            4194..4889
                     /locus_tag="ACHJ48_003720"
                     /pseudo
     CDS             4194..4889
                     /locus_tag="ACHJ48_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5565..7679)
                     /locus_tag="ACHJ48_003721"
     CDS             complement(5565..7679)
                     /locus_tag="ACHJ48_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO4709195.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
     gene            complement(7676..14017)
                     /locus_tag="ACHJ48_003722"
     CDS             complement(7676..14017)
                     /locus_tag="ACHJ48_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4709196.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLSEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANVIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREVTEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            complement(14017..>14639)
                     /locus_tag="ACHJ48_003723"
     CDS             complement(14017..>14639)
                     /locus_tag="ACHJ48_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709197.1"
                     /translation="VNDVYAGLWQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRN
                     CALRSDYSRRQALVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRII
                     FTPSKGLVGVGLPRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVS
                     VTFDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
BASE COUNT         3738 a         3736 c         3458 g         3707 t
ORIGIN      
        1 ttatctatat atcatgtgat atgttctgta acaagtaaaa tcaacataaa aaattcagca
       61 aaatttaata ttcgttcaac tagccgtctc acatatgaca ttattagtca actccatttg
      121 ccacaaaatg gctatttcat ggaacacagt aatttcttag tggctattat caagctaatt
      181 cttaggtccc tagcgattat tcctggctga ttcgtcagct tacgccatga gattgttaca
      241 ttccttttta tcctggagat atgggggaac gcaggagatg attttttcat ctacattcgt
      301 tggtaaatat agctttggtc actacgactt aaaccttcct caatcacagc cattcgccgg
      361 gcgatacggc aaacaaacta agtgaaacat catcctgaaa acaccaacat caataagcct
      421 ctccagatcg acttcagaag tgaccagtta caagccacaa accaggaaac atatttcatg
      481 atgagaatta tgctcagcct gatggcgggt aaacactgat ttacaccaag ctcactgagt
      541 tagcttgcaa agcttccctt gtttagactc taaataaatt atttataaaa caaatagtta
      601 aacgaagatc tttagttttt gaagaccacc gcaagtgttc gtctggcttc acatggcatc
      661 ttcttcttag aaaaagatcg acatattttg tgacacgaat tgcaaatctg gttttgttgt
      721 atggattgcg tgattttcga tctggtataa caggtataaa ggtgcaccac gatagacaat
      781 gagacagggc atcttgcaat ctatggcaaa catcacttca gttctttctc atcgggtgat
      841 gaaaacgcac ttcagcctga aaggaatatg aaaatgagat caacagacac tctattttat
      901 gactctgggt aaaatgaatt gagtaagtga tatagcttac gaacattcaa atcaattaaa
      961 catcagaata gattttatac tcaggtattt aatctggctc tctgtttatt taaataatgt
     1021 ggaaagagat ttttcacagg agacattata caaaaaatat aaaatacagc taccggttgc
     1081 caaagacact ataagcctgg caaaaaaata ttactcaaca taaatgctaa ttgtttatgc
     1141 gggctttgta ttgctttctg tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat
     1201 actttctggc gtattattac tgtgcttttc gtcaccatta atttctcagg ctgcgacact
     1261 ggacgtacgt ggtggatatc gtagtggaag ccacgcctat gagactcgac tcaaagtcag
     1321 tgagggatgg caaaatggat ggtgggcaag catggaaagt aatacctgga ataccattca
     1381 tgataataaa aaggaaaatg ccgcactcaa tgatgttcag gttgaagtta attacgcgat
     1441 taaacttgat gatcaatgga cggtgcgccc gggaatgtta acgcatttta gcagcaacgg
     1501 cacacgctac ggaccctacg taaaactgtc ctgggacgcg acaaaagatc ttaattttgg
     1561 cattcgctat cgttacgact ggaaagctta ccgacaacaa gacttatccg gtgatatgtc
     1621 tcgtgataac gttcatcgtt gggatggata tgtcacttac catattaata gtgatttcac
     1681 cttcgcatgg caaacgacgc tatacagcaa acagaacgat tatcgctatg caaaccataa
     1741 gaaatgggcg acggaaaatg catttgttct acaataccat atgacgcccg atattacgcc
     1801 atacatagaa tatgactacc tggaccgtca gggtgtttac aacggcagag ataatttatc
     1861 ggaaaacagt tatcgcattg gtgtgtcatt taaactgtag tagacaggag acagtcacaa
     1921 tgaataaaac aataacggcg cttgctatcc taatggcttc atttgccgca aacgcgtctg
     1981 tattaccgga aactcctgta ccatttaaaa gtggtaccgg agcaattgat aacgacactg
     2041 tctacattgg tttaggtagc gcaggtacgg catggtacaa gctggagaca caggccaaag
     2101 ataaaaaatg gacagcgtta gctgcattcc ctggtggacc aagagatcaa gcaacctcgg
     2161 catttattga tggcaatctg tatgtgtttg gcggcattgg caaaaacagc gagggcttga
     2221 ctcaggtatt taatgacgta cacaaataca accccaaaac caatagctgg gttaaattga
     2281 tatcgcacgc gccgatgggc atggcgggcc atgtgacttt tgtacacaac ggcaaggctt
     2341 atgttactgg cggtgttaac cagaatatct tcaatggcta ttttgaagat ctcaacgaag
     2401 ctggaaaaga ttcaaccgct gtagataaaa tcaatgcaca ctattttgac aaaaaagcag
     2461 aagattattt ctttaataag tttctgttgt cttttgatcc ctctacacag caatggagtt
     2521 acgctggcga atctccctgg tacgggacgg ctggtgcggc ggttgtgaat aaaggtgata
     2581 aaacctggct tattaatggc gaagccaaac ccggattgcg aacggatgcc gtatttgaac
     2641 ttgatttcac cggtaataat ttaaaatgga ataggcttgc tcccgtctca tcaccagatg
     2701 gcgtcgctgg cggttttgcg gggataagca atgattctct tatatttgcc ggaggggccg
     2761 gattcaaagg ttcacgagaa aattaccaaa acggtaagaa ctatgcgcat gaaggcctga
     2821 aaaaatcata tagcactgat attcatcttt ggcataacgg gaaatgggat aaatcgggtg
     2881 aattatcgca aggtcgggcc tacggagtat cattgccctg gaataatagt ctattgatta
     2941 ttggcggtga aactgcaggc ggcaaagcgg tgacggattc agttttgatc tctgtgaagg
     3001 ataataaagt cacagtacaa aactaacgct tcagggcccc ggtaaagggg ccctgctatc
     3061 aatttgtcat ttcaattacg cgaaatttat atgaacgcaa taatatcgcc tgattattac
     3121 tatgttctta ctgttgctgg tcagtctaat gccatggcgt atggcgaagg actgccatta
     3181 ccggacaggg aagatgcgcc tcatcccaga attaaacaat tagcgagatt tgcgcatacg
     3241 catcccggag gcccgtcatg tcactttaac gacattattc cactgactca ctgcccacac
     3301 gatgttcagg atatgcagag ttatcaccat cctctggcaa cgaatcatca aacacagtac
     3361 ggcaccgttg gccaggcact gcatattgca cggaaattac tgcccttcat tcctgataat
     3421 gcagggattc tcatcgttcc gtgttgccgt ggcggatcgg cttttaccgc aggcagcgag
     3481 gggacatatt cagaacggca cggagccagc catgatgctt gtcgttgggg aacggatact
     3541 ccgctatacc aggatttagt cagcagaacg cgagccgcac tggtaaaaaa tccgcagaac
     3601 aaattcctcg gcgtatgctg gatgcaaggc gaatttgact taatgaccag tgactacgcg
     3661 tcacatcctc aacactttaa tcatatggtt gaagcctttc gtagggatct aaaacaatac
     3721 cattctcagc ttaataatat tactgacgcg ccgtggtttt gcggcgatac cacctggtac
     3781 tggaaagaaa atttccctca tgcgtatgaa gctatttatg gcaattatca aaataatatt
     3841 ttagctaata ttattttcgt cgacttccag caacaaggtg caagagggct gacgaacgcg
     3901 cctgatgaag atccggacga tttaagcacg ggatattacg gttcagcgta ccggtcaccg
     3961 gagaactgga cgacggcact gcgaagcagt catttcagct cagcagcccg tcgggggatt
     4021 atttctgaca ggtttgtaga agcaattttg cagttttggc gcgaaaggtg agcgttcatt
     4081 aatttgattt attcgcatcc atcagcgctt ttaactcctc cggcgtgcgg cactataacg
     4141 acttccacaa caaggagcca ttttcttata ccactttcag tgaccatccg cttatgccac
     4201 caccctctac gcaactgttt gcagagcacc ttcccaccga gtggatacag cacttcctga
     4261 cgctttctgc tcatgcgacc gttcgtcgtc ttaacctgag cgtggatggc gaagcgggga
     4321 tgaacctgct ggcgcccgca gcgctgtcac ccaggcgcgt cagcgcgtgg gggccgcccc
     4381 ggtggaatgg ctcttccgcc agaccacaca gacatggggc gcggaacgtt acctgaagga
     4441 tgactggcac ggcctgcaac tttttgccat tgatggcgca cagttcagga cacctgatga
     4501 acccgagctg cgtgaatatt atggctctgc caacacatcc actgagcggc agagcgccta
     4561 ccccgtcatg cgtctggtgg cgttaatgaa tctgggaatc acattctact gaatgccatg
     4621 accgcgccta ccggcggagc gatacggcgt gaggcaacaa aagcggcgtc agagatcagc
     4681 tttaagttcg cgttccagtt tatcgtgaca gaaatgatag tgctgggaaa cacggcgtca
     4741 ccggggacta tcccgaaacg actggagcat ctgcgggggc tctggaagtg gtgttcataa
     4801 caaaacgccc ccgaccatca agggcgaggg cggttaagat atcaaaaacc cgttatcctg
     4861 tgaaacacag tgccgccccg cttaagtgaa cggcattgtg tttctgaggg accttaattt
     4921 tccctgattc tctgacgttc cttaccattg atatttcctt cattcctcac cagagaaata
     4981 aaaaatcact atactcatca aaatatgaga ttacctttat tcaggtgatc accttactat
     5041 tttttacgga aaataaatta tgaataacaa gaaatcactg acaaaccacc tgtaaaatgt
     5101 caatcatttt agttcaattg cctttgtcat gattatcttt ttatcgtcct gacgatacta
     5161 ttaacaattg caatttttat acccctcctg aacaaataga aaagaactaa tatattcgtt
     5221 tataattctg gtaatcctgg catcatacgt ttaaaactta agatatttat tttatcatca
     5281 tcatggttcc agagactatt ggtcagctgg aaatgccagc cttttcgtgc cagctatatt
     5341 tgtccagtgt gtcaccccat ccaaaaatca tccacatcct gacgttacgt tagatataga
     5401 gaaaacaggt tgtactgtgc aatatgcaaa ccgcataagc gaatataatt tctaagttga
     5461 tattgtctgg ctgttgggag ctaagcaata taaatatcgc cagttgccag aacggttata
     5521 tagaccagga gcgtttcggc tcctggcctg ttttggacat attatcagtc agccactaat
     5581 agcggactag ggacaccaga tgatgaaaca aacagccctt ttacggcacg ggttgcagcc
     5641 acatgcagta acgcgcgctc attaaataat gcatcacgct gttcaacggg atcatcggat
     5701 gctaatgctt ttatttccgg cacaacgcct tcgttaatcc ccaccaggaa cacatactgg
     5761 aactcaagcc ctttaatacg gtgcatcgtt gcaacccgca cgccagggcg tgcatcgctg
     5821 tcaccgctat cattgcctaa attaaatgtc tcaattccgg cgtcattcag tgctgatgcg
     5881 tagcggtcac aagcatgttt agtacgcgca gtaatgcaga tatcctgaga acgaacctga
     5941 ttcgccagca atgcctgaat ttgcttgacg agcgttgcag cctcttcttt gaaatctgcc
     6001 gcatgcgtaa tcatgggctt ttccccgtgc agtaatgaca aataatcatt actggtatcc
     6061 gcttcgccat cgaggttatc aaccttcaca ccagttaaca gcccaacagc aaattgacgc
     6121 gtctcttctg tggtccgata gttaatcttc aattttttac tgcgacgacc acggacgtta
     6181 ataccacact gaccaagaac gaccttattg cgataaatac gctgatgccc atccccgaca
     6241 ataaacagat cgttgggcga ctcgggaacc aggctgcgaa taagagtaaa tgccggggca
     6301 ccgatatctt gagcttcatc gaccacaata ctgctataag gtagttggac ctgcttttct
     6361 ttaaacagaa caatagcctc atgcatggca tcgcccattt cgcgcagttt ggcacgcgcc
     6421 atttgtgctc tgaattcttc aaagactggc cagattttcg cccgttgaat acgggataac
     6481 gctgtaccac gtccaacccg gctgactttg aggtattcct cccgggtgta aaccgcattt
     6541 ggctggacaa cgcgttgcca ctcttcagca tagaaattat caggtagccc cagttcggca
     6601 ggtgcctgct gcaaggcata gttccagcat ttgcgtcgcc cttcatcgct gtcgtatacc
     6661 acgcggaaat cataaccgtg gcgttttagt tggtcgctca tccaggcatc aatgttaacg
     6721 acgtcaatac gcgccatctc ttctcgcgta cacaaccgct ggagattagc gcgaatatct
     6781 gccgccaggt tacgcgtaaa ggtcgtgaaa agtactttct taccgggttt atctgccaga
     6841 cgctgagata accaccgcgc acggtgcatc gcaacaaccg ttttccctgt acctgcaccg
     6901 cccagtactc gtaccggacc attagcgggc gactccacca attttcgctg gctcggatgc
     6961 agaaatacac gccatttttc taaaggcgca ttaagcatct gcaacagctc ctgttcattt
     7021 tcaacgacat ggaagcgacg tttacttcgt tcaatcgcct ccataaagtc gttggtatca
     7081 acaggctcat cacgatgatc gttaaactct tccagcactt cctggtaatc cagcccttct
     7141 gccagccagt gcagaggttc ccaggcttct gctggcatta tgctttcaag ttgattaagc
     7201 ccggcttcat cggtaagcgc catcacctga tcaataaaga caggcggcac acccagagcc
     7261 agaatctgtt ccgcgctgta agcagcgaag agtttcggtt tatcaaccac tgtttccgct
     7321 gccggtttga tatagctaat atcaatgacc tgaatcgcgc cggtaaccgg gtgaattgaa
     7381 caatcatgcc gccgcgccca gtcatatgct tcgtcgtgtt tatccaccca catcagcata
     7441 tacagggcac cctgttcagg tttcagaaca atgccgcgat aagtttgatc gattcgcacc
     7501 gaatggacat ttttactgcg cgcatcgtgg atcttttcat agttaagtcc attactggtc
     7561 gggtcctgac ggaatttgga gatgaattcc tgcacctttt tttgctgtgc ctttgggatc
     7621 cgcgcgaatg cgcccagaaa accatcagaa agtgccacct tgggcgtttg caccgtcatt
     7681 tgtcacctcc ggacagatgt tgcaatgtat tttcgtcaat tggcccgaat gcaacgtgcc
     7741 aacccctggt tgtgaataat ggaataaatt cttgattatc aataatcaat gcccattttt
     7801 gtgatggcca ggcgagatcg gcttccgcaa taatttcacc ttcatcattt tgcaattcgt
     7861 agccgagagt cggcgcagag agagaatgag actgaagcaa cgcgatctct tcagcgctta
     7921 ataagccaaa ctcaataatc tcagcccaac tctcatcagt ctctggtact gcctctggcg
     7981 ttgccgaaac tttagtgtca acgctttcct gcggtaaatg cacggcttta cggctggtaa
     8041 aggtcatatc cggcaggaac tgtaacaggt ttaccagtcg ccagaagcca ttgagtcccg
     8101 cttcataacc gtcatcctgc gtatagcgat cgtcaaaaca gatatgcaaa cgcagccagc
     8161 tgcgcatttg tgcgacgctg gtcgtcggtt taatggcttg ttgtggtaac accgaagcca
     8221 gctcaataaa ttgctgcgat gagttacagc tatcgagtaa accaccaaag acaaacggtt
     8281 cgtctggcaa aagctctgct agccgccaag ctggcgcgtt ttcctgcatt tcataggcat
     8341 acttttgttt cgttcccgcg tcctgtgact tcttcacatc caaccacacc caggcatgtg
     8401 cagcagccat tctctgccat aactgcgttc ttccgcccgg atcggcgaga taatccagca
     8461 gcagtgcaaa actgttgcgt tcccggaacg acgcttcaag ggccgcaaag ttggtatcat
     8521 gaaacaggtt ataaaactta ggctgtttca tatccgggtt atgcccaagt cccaggacat
     8581 cctgaacatg tttaagccct ggctctttga gatcggccca cgtgactgtc cacacccaga
     8641 aattaccgct atcccgaatc gcctggcgtt tttgcacatc ctctgcgacg ctatttttgt
     8701 ggaacgcaaa gccatcaagg aaaatagcca ccggtttaat ggcatcagac tgcataagcg
     8761 gatatagcac gtaatccggg cgacaaggga tccccaccgc ctctttagcg cctaaatcca
     8821 cctgagcttt aagatgccag ctcatcatcg gttcgctgcg ggtgttaata atccacccag
     8881 cgccctgatg cgcgtaactg cgactgacaa tcaggttttt attttcttgc aggcagctaa
     8941 taaaccgttt ttccagctca ctgcccatca tggcttcgag ggaaatgttt ttgatggaat
     9001 caattacccg tatcgaatct cttgcctgca aaattttcgc cagcaagaga cgcgcctgat
     9061 cgcgggagac atacttcatg cgcccacggt cacggtaagc gtaaacgcaa cgataacaac
     9121 catctttatg cgtatcattg ttgcaactgc attccactat tgcctgatag gccagttcaa
     9181 gtaatttcag caaattgtcg ggagtgcgca tcagttcttt aagagaacca gtcccccccg
     9241 ggacggtatc gtaaatcacc agatactgcc gccaggattc gccttcattt tccggctcgc
     9301 ggtacacaac gcctttcagg tgatcgacat tccccttgaa gtaatgttta agaccgagct
     9361 ggatcgccgc cccaagagaa gcctccacga cacggtcatt actgtaactg cttaccggga
     9421 gcagaatacg caacgcttct gactccagct ggcgatagag gtagagataa tcttcgaact
     9481 tcgccttttc cggttccgtg cgatatttgc agctcaaatc atgacgtggt tcatgatcgc
     9541 gaggacgctg caccatgccg cacccgaggc aaactttaaa cccggttcgc ttcttcgctt
     9601 ctccggcaat catcaactca ttggcatcat ccgccatttt gccgaagttg atatcccgca
     9661 gggtgacttt gcttaaaaat tcaaaaccaa acggaacttc gccttcctca atcgcgtatg
     9721 ccgccgatac atcttctttt tcgaaactaa ccaatagctg acgctggaag aaagcaggtt
     9781 cacggctgtc gctctcgtcg ctgatctggc tatcacgtgc gctggcgcgg gcataaacct
     9841 ggcgaagttt cagcagcgtg gttttttgtc cggcatcggc ccaccccggt gtgccacatt
     9901 tcgggcaata tttatgctgg tcgcccgtct ggtcgatatt ttcgctgtag ttacagtggc
     9961 tacagatacg ccagttctcc ggctcagaga ccttcaggtc tatctgctca atttcaactt
    10021 tatgcccacc ggcatagaaa ttattcagcg gagccagctc agccagcgcc gttgatgccg
    10081 ggcgttcata ttcgaaagtg gtgttttgat attcacgtgc ttccccgcct tctttacgcc
    10141 gccagagcac agaacgtaac gtaataccgg cttccgggaa ggcgtagtta ggcagcagac
    10201 cttcatccgt gaggaaattc agcgtttgct tattgttgat ttgattgacc agcgccatca
    10261 gcgcctgccg ttccgaagtt aactcgcgca tatcgctatc aaagttttga tcgcgcggcg
    10321 cgttctccag tttatcgata ctgcgcttga gtttatcgat gcgtgagcgc aaagatttgc
    10381 gatcttccac cagcagtttt aacgcttctt cgatacgctg caccagcgag cgttgtcctg
    10441 gcgcgccctg aagataagac aacagctgtt ggcgagtttc tgcggcaaga tcggggaaga
    10501 ttgccgtaaa ttgcgcagca atctccgcat gatgctgttc gatatatcgc agtaagttat
    10561 acggaaaacc cgatttacgc ccgaactcca gctcatccag catctgtttg acgtttttac
    10621 agatagcgga ttctggtaca ccggttttca cccagttatc catacaaaaa gctgccagct
    10681 ggcgttcgag aatagccgtt gcattaagga agacgccagg agcctgcacc tggccctgca
    10741 tcatctccag tggttcttca aagaaaaact gatcgtgcgg gttcccttct gccacggtaa
    10801 tgtttaacgc gttaccatct ttacgaccgg cgcgaccaat acgctgcaag tagttggcct
    10861 gagcaggtgg gacagaacag agtaaaacgg tggaaagatc cccaacgtca atacccatct
    10921 ccagcgtcgg tgttgccgac aacaggttga tattccacgg ttggttgccg cggtagaacg
    10981 atttttcggt gacttcgcga tcttcacggc taagcagccc cgtgtgttct gcgccctgta
    11041 cgcgcgcgat gtccatgttc tcccaacgcc aggtcgccgt attgtcgact tcctggtaac
    11101 gaccttcaca acgtaaagag agacaaggcg cattacgcca ctgccagcct tgatcggccg
    11161 gagcccggac aacctcacgg caacatgagc aattaagctg aacgacgtca cggctcacca
    11221 ccagcgccga aggcaccagt ccccaggctt cacgcccttt ggtgtcaaaa tcttttacta
    11281 ctccggctat tttcaacgcc gtaaacaggc gtcgtaacac tgtttccgca acgctactga
    11341 taaacagatt attgccgtta ctcagcgtgc gattgatcca atgctgatac caactggggt
    11401 tagacttcgc gccaatcagg cgatggaaac cattaatttt ttcaaacgac gcataagccg
    11461 gtttacgcgt tttcggaccg atagaaggca tatagcgcga ggtacgctct ttttgcgtca
    11521 tcataaaggt cgtgccatca cggcgataac tttcgaacgc cggatgcata acggccccgg
    11581 cctggcgttg ataccagata acccccagcg ccaggtgcgc tacctgctcg acgctcacac
    11641 cagacatctc atcacccagc tcttcacaaa gctgttgatg cagggcggta attgccggat
    11701 tgaagcgatc tttatccact ccggcaacgg cataaccggt ggcttccagc gtacgtccga
    11761 tacgcgcgcg cagggtaaat tcgctgtaca cctcccagtc gaggcgcagg gaaaccagct
    11821 ccggcagatc ggagttctcc gccagtttgc cttcattttt cagcgtctga taatcattca
    11881 gccactgcat atttggtgcg atgacgtttg ctgcaaaatg agcatcgtcc ggtgaacgtt
    11941 cacgcgtttg ttggcaaaca cgcgccgcga acgcatccag cggtaatgtc tctttttcgc
    12001 taagaacact ggcgatttgc ccacgaatca tcagcggcca ggtacgggca gtaaagaagc
    12061 tggcacgatg cgcggcgtct tgtaccgagt cagaaaacgt aatcagcttt ttatgttcgt
    12121 tgaactggct gccataaagc tggtgaatca tcaccgccga cagactggca gcccgtgacc
    12181 ccagaatcga aatcccctgt ttgctgttgc agtacgggca ttcattagca aactccagct
    12241 cgtccttacg ctctttgagc atatccggga tcagtacctg caacagttcc gtatgaccgc
    12301 aatgtccaca ctggacgctg gactgtcggt tcagtttcag acaggtagga cacagtttct
    12361 ggtgtgcgcc actgaccgct ttttcgttgt tatccgggaa agcaaggacg atggagcgcc
    12421 ctttttcaaa gaactggcga taaatgccgt cgaggtctgt ttccaactga acgctttgcc
    12481 catgacgcag gctggcccag ccagttgcgt ggcagtcacg gcagtggata accggcaaat
    12541 agtgttcgcg atcttccacc gccacatcat cggcaaacac cagtcggggc gttttcgcca
    12601 cgctggcgac cagacgacgc atctcacgaa gccacaactg ggagcgcagt tgcacaaacg
    12661 gcagcaccgg acgcgctttc ttcgcctgga gacgtttttg ccgatcgtgt tccgtttccg
    12721 gtacttcgag acgtgcctgc gcgatcagtg ccagaatgct ttgcagcacg cgtcgggcat
    12781 ggtcggcgct ttctgccagc atgacctgga gattttccag acactctcgt tcagagagga
    12841 tcccgccttg cagatcatgg agtaagacat gcatcacgct gtggcggcgt aacagtgacc
    12901 ccagcgcaat acgcttttca cgcccgtcgt cgctctccag atcgtccggg agttgcaagt
    12961 cgctgtcggg gaaccacaaa gggatttgcc cgttaagata cgctaccggg gtagcgtaac
    13021 tctgcgggtc cagggccgaa gcgacctccg gccccggata ctgactgtac gcaatggcgt
    13081 aattctgcaa gtactccgct gccgtgtagc gatcttcgcg aataacggcg tcatcggtaa
    13141 acggctggtc aaagattgtt tttgcgtaat cgagcagttg ccccagttcg tcgccgacgg
    13201 tagctgatgt cccgacgcag gcgaatcgtt ggttatccac acctatgtga tgcttcaaac
    13261 gtcgcaccag acaggccaga tccgaccctt gcgcgccatc aaacgtatgc aattcatcca
    13321 cgaccaggta acgcaatgaa ccgggctggt tgtaacgcca gagcggctga tcgccagggc
    13381 gcatcagcag gtagtcgagc attttgtagt tggtcaacag gatgtccggc gggctttcgc
    13441 gcagcgtgtg cttacaggta atgacctttt ctgccgacat ttttttgctg ggttcgattt
    13501 cgctgtcgcc gacaaacagg ccgacggtca ccttgccatg cagttgtgga tcgctggcga
    13561 tggttttggc gaaacggctg gcctgatcgg ttgccagggc gttcatcggg tagatgataa
    13621 tcgctttaac cccggcttgt gacgcgcccg cgcagtggtt gagcagcggg aacataaaac
    13681 attctgtttt accggagccg gtaccggttg cgaccagcgt cggctgtggc gcgtcgcttc
    13741 ccagacgctg aaatgcccgg gcctgatgcg cgtgcggcgg gaacggcagc gtcagattag
    13801 ggaaaaaatt cagcggtaag tcacttttac gaaacggcaa ctgggcggaa aggtatggtc
    13861 ctttcagcag cgtttccggc tgggccagaa attgctccag cattgagaca ttatcgttat
    13921 tctcaccgtt aaacagcggg ctgtttagcg ggaacgccgc ccgtaaaaag gcggcaacgc
    13981 tgtcggcgac ctgccgactg acaacggacg gcaacatcag gcgctctcct tatcttgtgc
    14041 aaagaacgcc caggccactt tgtagtcatc ttcgcgatct ggcttgataa acggagcctg
    14101 ccaggtgacg gtacggcgct caccttcgtc gctgcgggta taatcatcaa aggtgacgct
    14161 gacggtaccg gttttaagat gtttgacatc atcccaaccg atagcttgat cggtgcagtc
    14221 accgccggtc cactccgggc tgtcgacgtt aaagacaaag ccgtttttca ggtcagcttt
    14281 acgcgcggtg cgaggcaagc caacgcccac cagccctttg cttggggtaa agataatgcg
    14341 accgttttga tcgtaccagg tatccgcttc gtactggcgc atcaccggga actgaacgcg
    14401 ataaatggta agcagctctt cgagagttaa ccccagcgcc tgcgccacca atacgtcgat
    14461 ttccaccagc gcctgacgac gactgtagtc agagcgtaaa gcgcagttac gctgccactc
    14521 tggggtcagt ttggcgaaga aatcctgggg gagctgcggg agatcgcggc tccaacgctg
    14581 ggtgttgaag tctggggtgt agcaggattg ccaaaggcca gcatatacat catttacag
//
LOCUS       DAZFDC010000119        14200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFDC010000119 DAZFDC010000000
VERSION     DAZFDC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.119"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..586
                     /gene="yfiR"
                     /locus_tag="ACHJ48_003724"
     CDS             68..586
                     /gene="yfiR"
                     /locus_tag="ACHJ48_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO4709198.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            576..1802
                     /gene="dgcN"
                     /locus_tag="ACHJ48_003725"
     CDS             576..1802
                     /gene="dgcN"
                     /locus_tag="ACHJ48_003725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO4709199.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLP
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSAA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYGVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1818..2300
                     /gene="yfiB"
                     /locus_tag="ACHJ48_003726"
     CDS             1818..2300
                     /gene="yfiB"
                     /locus_tag="ACHJ48_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO4709200.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITPP
                     "
     gene            complement(2377..2724)
                     /gene="rplS"
                     /locus_tag="ACHJ48_003727"
     CDS             complement(2377..2724)
                     /gene="rplS"
                     /locus_tag="ACHJ48_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO4709201.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2766..3533)
                     /gene="trmD"
                     /locus_tag="ACHJ48_003728"
     CDS             complement(2766..3533)
                     /gene="trmD"
                     /locus_tag="ACHJ48_003728"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO4709202.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3564..4112)
                     /gene="rimM"
                     /locus_tag="ACHJ48_003729"
     CDS             complement(3564..4112)
                     /gene="rimM"
                     /locus_tag="ACHJ48_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO4709203.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4131..4379)
                     /gene="rpsP"
                     /locus_tag="ACHJ48_003730"
     CDS             complement(4131..4379)
                     /gene="rpsP"
                     /locus_tag="ACHJ48_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO4709204.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4516..5877)
                     /gene="ffh"
                     /locus_tag="ACHJ48_003731"
     CDS             complement(4516..5877)
                     /gene="ffh"
                     /locus_tag="ACHJ48_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO4709205.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6044..6835
                     /gene="ypjD"
                     /locus_tag="ACHJ48_003732"
     CDS             6044..6835
                     /gene="ypjD"
                     /locus_tag="ACHJ48_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO4709206.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6857..8143
                     /gene="yfjD"
                     /locus_tag="ACHJ48_003733"
     CDS             6857..8143
                     /gene="yfjD"
                     /locus_tag="ACHJ48_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO4709207.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVVGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8198..8791)
                     /gene="grpE"
                     /locus_tag="ACHJ48_003734"
     CDS             complement(8198..8791)
                     /gene="grpE"
                     /locus_tag="ACHJ48_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO4709208.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8914..9792
                     /gene="nadK"
                     /locus_tag="ACHJ48_003735"
     CDS             8914..9792
                     /gene="nadK"
                     /locus_tag="ACHJ48_003735"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO4709209.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGATRTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9878..11539
                     /gene="recN"
                     /locus_tag="ACHJ48_003736"
     CDS             9878..11539
                     /gene="recN"
                     /locus_tag="ACHJ48_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO4709210.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IVEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPETLPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKTRLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11688..12029
                     /gene="bamE"
                     /locus_tag="ACHJ48_003737"
     CDS             11688..12029
                     /gene="bamE"
                     /locus_tag="ACHJ48_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO4709211.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12091..12381)
                     /gene="yfjF"
                     /locus_tag="ACHJ48_003738"
     CDS             complement(12091..12381)
                     /gene="yfjF"
                     /locus_tag="ACHJ48_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO4709212.1"
                     /translation="MPGKIAVEVAYALPKKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTENKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12371..12808)
                     /gene="ratA"
                     /locus_tag="ACHJ48_003739"
     CDS             complement(12371..12808)
                     /gene="ratA"
                     /locus_tag="ACHJ48_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO4709213.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            12979..13461
                     /gene="smpB"
                     /locus_tag="ACHJ48_003740"
     CDS             12979..13461
                     /gene="smpB"
                     /locus_tag="ACHJ48_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO4709214.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13676..14038
                     /gene="ssrA"
                     /locus_tag="ACHJ48_003741"
     tmRNA           13676..14038
                     /gene="ssrA"
                     /locus_tag="ACHJ48_003741"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3581 a         3753 c         3354 g         3512 t
ORIGIN      
        1 tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag atggtgggga
       61 tccctgaatg cgtttttctc accgactgtt cttactgctg attctcctgc tgacgggagc
      121 accaatactc gctcaggagc cttctgacgt tgcgaaaaac gtgcgtatga tggtttccgg
      181 tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat gtatcttttc
      241 atcttcacgt tttagtaccg ccctacaaga gaatgccgca acatctttgc cttatctgcc
      301 tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt tttattttgg
      361 caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa aggcgttatt
      421 attaattgcc gaacaaaata ccgagtgcat tattggtagc gccttttgtc tgatcatcca
      481 caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg gtgtaaaggt
      541 caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat aattctctta
      601 ataagcgccc cacgtttaaa agagcattac gcaacatcag tatgaccagc atatttatca
      661 ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgcccctg aaacagtacg
      721 cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa gcagctgtcg
      781 tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag caagggcaat
      841 tttcaactgc agaagtacgt gataagcagc aaaatattct ggcatcctgg cattacaccc
      901 gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc cctgccccca
      961 tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta accgctcgcg
     1021 acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt tgtattctgc
     1081 tggcatcagg aatcgcaatt accctcaccc gccatttgca caatggcctg gtggaagcac
     1141 tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt tcccgacgag
     1201 tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac agtctgctgg
     1261 atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt ctacgtaccg
     1321 cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc ggcatcaata
     1381 cgttgatgaa caattccgct gcccgaaaaa cgtcggcgtt actatttctt gatggcgata
     1441 atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagagtc ttgattgaaa
     1501 tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc ctgggcggcg
     1561 atgaattcgc tatggtgctc tatggtgtac agtcggaatc tgaagtgcag cagatatgct
     1621 cagcactgac acaaatcttt aatctcccgt ttgatcttca taatggccat cagaccacca
     1681 tgacattaag cattggttac gcaatgacca ttgcgcacgc ttctgcggaa aaattacaag
     1741 agctggccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag ctggtgagat
     1801 aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc acctcactaa
     1861 tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc gccgctatgc
     1921 aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca gatgccattc
     1981 tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc caaaccatgg
     2041 cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac accgataact
     2101 atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc gtggccgatg
     2161 catgggctat gggtggacaa attccacgca gcaatctcac cacacagggt ttaggaaaaa
     2221 aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc cgcgtcgcag
     2281 tggtgattac tcccccttaa aacacgagca aacagataaa aaaagggcca gccaattggc
     2341 cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct ctttgatacg
     2401 agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa cagcaccacg
     2461 acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac gctcaacgcc
     2521 ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt tacgaatagc
     2581 gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa cccatacttt
     2641 cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct gctcttgttc
     2701 aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac tgatatctcg
     2761 ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt tttgaactcc
     2821 gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc aggtcttcta
     2881 agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc atggttgccc
     2941 gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt atagtgcggg
     3001 caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg tcccagtact
     3061 cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc accaccactg
     3121 agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac gcgctcatct
     3181 ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag ttcgctgacg
     3241 cccgcttgat caagcttgcg tccctgtggt gaaagataaa tcacctttgc gccttcaccc
     3301 gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat taacatcccc
     3361 ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt gaaatcgcga
     3421 ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt taccccgtaa
     3481 tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca catagcgccg
     3541 tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc gattgaacga
     3601 gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag acgttccttg
     3661 ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattgga tccggtttcc
     3721 atcatatcga cgactttacc gagatcgtag ccttcagtgg ttactacctg gcagcccatc
     3781 aggtctttcc agtagtagtc accctcttca agctgaggca gctgcgatga atccacgaca
     3841 atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt cagcttgatg
     3901 atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca ctgacccgcc
     3961 ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt ggaagaaaac
     4021 actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac gatgggatca
     4081 acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga ttaagctgct
     4141 ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc ctggccaacc
     4201 cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct agcgattggg
     4261 ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct gtcagcgaca
     4321 acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat agttaccata
     4381 acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc cggtgtcata
     4441 ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt taataactct
     4501 gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcatcccct tcatgcttct
     4561 catcatcttc gccattccgc ccttcttcat tttcttcatc atgcgctgca tgtcgtcgaa
     4621 ctgtttcaga agacggttaa cgtcctgcac ctgcataccg caaccggcag caatacggcg
     4681 tttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca tcgagttgat
     4741 gatggcttcc atacgcacca gcactttatc gtccatctgt gacttgacgt tatccgggat
     4801 ctgccccatg cccggcagtt tgcccatcag gctggccatg ccgcccatat ttttcatctg
     4861 gcgcagttgc tcaaggaagt cgttgagatc gaagccgtca ccttttttca gcttgctggc
     4921 taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca gcgacagtac
     4981 gtcgcccatg ccgagaatac gcgacgcgat gcggtccgga tggaacggct ccagcgcctc
     5041 agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac gaatagagag
     5101 cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg taagcggtaa
     5161 cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg cgtcaaccac
     5221 aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt ccatcatcgc
     5281 ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga atttcagttt
     5341 ggcttctttc agcgccgcgt taacaatatc aaccggcttc tgaccaacat cagaagggaa
     5401 gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg ccgggcgata
     5461 gacgtcagca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga acttaccgag
     5521 tttaccaacg ctggttgttt taccggcacc ttgcaggcct gccatcagca cgaccgcagg
     5581 cggttgcgca gccagattca gggtctggtt ctcttcgccc atcgccgcaa ccagttcgtt
     5641 acggacgatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt catgaccaac
     5701 cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga cgtccgcctc
     5761 cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag tgaggcgtcc
     5821 acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat caaacattgt
     5881 ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt attttgccgc
     5941 agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt cacctgacgc
     6001 tatactgctt ctctttctta ttgctcaaac tgtcgacatc actatgcccg tttttgctct
     6061 gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc tgctgcaaaa
     6121 aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg tctgccacgc
     6181 aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc tcagcctgct
     6241 gaacgttggt tcattggtca gtttgatgat ctgtacggta atgaccattg tggcttctcg
     6301 caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca acctggcgct
     6361 ggcaaccttc atgcccaatg aatacatcac ccatctggaa gctacgcctg ggatgctggt
     6421 gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc tgtacgcgct
     6481 gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcgttta accaggaaat
     6541 gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg gcgtggtgct
     6601 gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacttattta gcatggaaaa
     6661 tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc tgctatgggg
     6721 acattatcat gaaggatggc gtggacgtcg cgtcgtctgg tttaacgttg cgggtgcagt
     6781 cattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca gctaaaccca
     6841 gaaaaggagt ttccccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
     6901 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
     6961 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
     7021 gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat
     7081 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
     7141 aattgcgact ggtgtgctga cttttgtcgt actggttttt gctgaggtat tgccgaaaac
     7201 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctcccct
     7261 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
     7321 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
     7381 cactatcgtg cacgaatcgc gctcacaaat ttcccgtcgc aatcaggata tgctgctgtc
     7441 ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtgccgcgca gtgaaattat
     7501 cggtattgat atcaacgatg actggaaatc tattctgcgc caactctccc actcacctca
     7561 cgggcgcatc gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgagtacg
     7621 tgaagcttgg cggttgatgt cggagaaaaa agagttcacc aaagaaacca tgctgcgcgc
     7681 cgcggacgag atctattttg tgccggaagg tacgccgctc agcacgcagt tggtgaagtt
     7741 tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca ttcaggggct
     7801 ggtgacggtt gaagatattc tggaagagat tgtcggcgat ttcactacgt cgatgtcgcc
     7861 aacacttgcc gaagaagtca cgccgcaaaa cgacggttcg gtgattattg atggcactgc
     7921 caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg atgcccgcac
     7981 ggttaatggc gtcattcttg aggcactgga agagatccct gtcgtaggca cccgcgtgcg
     8041 tattggcgag tacgatatcg atattctcga cgtacaggac aatatgatta agcaggtaaa
     8101 agtttttcct gtgaaaccgc tgcgcgagag tgtggcggag taacgaaaac ggcccggcat
     8161 tcgcatgcag ggccgtgaat tattacgaaa acagaaatta cgcttttgct ttcgctacag
     8221 ttaccatcgc cgcacgaatc gtacgaccat tcagcgtata acccttctgc ataatgccca
     8281 gtacgttacc tggcgcaacg tcatcagatt ccaccattgc gatagcctga tgcacattcg
     8341 ggtccagcgg cacgttagtt tcggcgatca cttcaacacc aaacttacgc acaacatcca
     8401 gcatcgactt cagcgtcagc tcaatgcctt caaccatcgc agacatatcc gggttagctt
     8461 tatcagccac ttccagcgca cgatccaggc tatcaatcac cggcagcaat tcgttgatga
     8521 atttctccag cgcgaatttg tgggcttttt caatatccag ttcagtacga cgacgcaggt
     8581 tttccatttc ggcttttaca cgcaaaatgc cgtcacgttc acgggtctgg gcttcagcca
     8641 gctgagcttc gagattcgca attttttcat cgcgcggatc cacctgctca gcagaagctt
     8701 ctggctcaac tgcctcaatc tcttcgtgct gatccatgat aatttcttcc ggggcttgcc
     8761 cctcaggcgt tttctgttct ttactactca tgaatttctc cgcgtttttt tcgcattcat
     8821 ctcgctaact tcgcttatta tggggatcag tttcagggtt tcaagggaag cactcacatt
     8881 gtcatcaatc ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa gtgtattggc
     8941 attgtgggac acccacggca ccctactgca ctgacaacac atgaaatgct ctaccgctgg
     9001 ctgtgcacaa aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga actgcaactg
     9061 aagaatgtga aaactggcac gctcgcggag attgggcaac tggctgatct cgcggtagtc
     9121 gttggtggcg acggtaatat gctgggcgcg acgcgcacgc tcgcccgtta cgatattaaa
     9181 gttattggaa tcaaccgtgg caacctgggt ttcctgactg accttgaccc cgataacgcc
     9241 cagcaacaat tagccgatgt gctggaaggt cactacatca gcgagaaacg ttttttgctg
     9301 gaagcgcaag tctgtcagca agattgccaa aagcgcatca gcaccgcgat aaatgaagtg
     9361 gtacttcatc caggcaaagt ggcgcatatg atagagttcg aagtgtatat cgacgagatc
     9421 tttgcgtttt ctcagcgatc tgatgggcta attatttcga cgccaacagg ctccaccgcc
     9481 tattccctct ctgcgggcgg tcctattctg accccctctc tggatgcgat taccctggtg
     9541 cccatgttcc cgcatacgtt gtcagcacga ccactggtca taaacagcag cagcacgatc
     9601 cgtctgcgtt tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag ccagatagca
     9661 ctaccgattc aggaaggtga agatgtcctg attcgtcgct gtgattacca tctgaatctg
     9721 attcatccga aagattacag ttatttcaac acattaagca ccaagctcgg ctggtcaaaa
     9781 aaattattct aattttacgc cagcctcttt actgtatata aaaccagttt atactgtaca
     9841 caataacagt aatggttttt catacaggaa aacgactatg ttggcacaac tgaccatcag
     9901 caactttgct atcgttcgtg agcttgagat tgattttcat agcggcatga ccgtaataac
     9961 tggcgaaacc ggtgcgggta aatctattgc aatagatgcc ctcggtcttt gtctcggtgg
    10021 tcgcgctgaa gccgacatgg tgcgtaccgg cgctgctcgc gctgacctgt gcgcccgttt
    10081 ttctctgaaa gatacgccag cggccctgcg ctggctggaa gaaaaccagc ttgaagacgg
    10141 gcatgaatgt ttgcttcgtc gcgtgatcag cagcgatggt cgctcccgtg gtttcatcaa
    10201 cggtacagct gttcctctgt cacaactgcg cgaactgggt cagttgctga ttcagatcca
    10261 tggtcagcac gctcatcagt tactcaccaa acccgagcac caaaaattcc tgcttgatgg
    10321 ctatgccaat gaaacctctc tactccagga aatgaccgca cgttatcagt tgtggcatca
    10381 aagctgccgt gacctcgcgc atcatcaaca gttaagtcag gaacgcgctg cccgtgcgga
    10441 actgctgcaa taccaattaa aagaacttaa cgaatttaat ccgcagcccg gagagtttga
    10501 gcaaatcgac gaagagtaca aacgtctggc gaacagcggt caattgctga ccaccagcca
    10561 gaatgcattg gcattaatgg ccgacggtga agacgcaaac ctgcaaagtc agctttacac
    10621 ggctaaacaa ctggtgagcg aattgattgg catggacagc aaactgtccg gcgtacttga
    10681 tatgctggaa gaagctacca tccagattgt tgaagccagc gacgaactgc gccactactg
    10741 cgatcgtctg gatctcgatc ccaaccgact gtttgaactt gaacagcgca tctcaaaaca
    10801 gatttcgctg gcacgtaaac atcacgtcag ccctgagaca ttgccacagt attaccagtc
    10861 gctactggaa gaacagcagc aactggacga tcaggccgac tcacaagaaa cgctcgcact
    10921 ggcggtaacg aaacatcatc agcaggcact ggaaacggcg cgcgcattac accagcaacg
    10981 ccagcattat gcagaagaac ttgcacagct gatcaccgac agtatgcatg cgctctcaat
    11041 gccgcatggg cagtttacga ttgatgtcaa atttgacgag catcacctgg gcgctgacgg
    11101 cgccgatcgt attgagtttc gggtaaccac caacccaggt cagccaatgc agcctattgc
    11161 caaagtcgca tccggtggtg aattgtcccg catcgcattg gcaattcagg tcatcacggc
    11221 gcgtaaaatg gaaaccccgg cactgatttt tgatgaagtg gatgtaggga ttagcggtcc
    11281 aacagcggca gttgtcggca aactgctgcg tcaactcggc gaatcaactc aggtgatgtg
    11341 tgttacccac ctgccacaag tcgcgggatg tggtcatcaa cactattttg tcagcaaaga
    11401 aaccgatggt gcgatgacag aaacgcatat gcaatccctg aataaaaaaa cgcggttaca
    11461 agagctggcg cgcctgcttg gtggcagtga agtcacacgt aacacactgg cgaatgcgaa
    11521 agaactgctt gcggcgtaaa cttttttcct gcttcacggt cagagtaaac agcaaaacgc
    11581 cgtaagaccg gaaagcaaaa ggttttaaag tgatgaaagg tctattatca tcggcatatt
    11641 acagatgagc cacgtactgc tcgggcccga aaaggaatca aatcactatg cgctgtaaaa
    11701 cgctgactgc tgcagcagca gtactattga tgttgaccgc aggctgttcc actctggagc
    11761 gagtggttta ccgtcctgac atcaaccagg ggaactatct gaccgctaac gacgtatcca
    11821 aaatacgtgt tggcatgacg caacaacaag ttgcgtacgc attgggtaca ccgctgatgt
    11881 ccgatccatt tggtacgaat acctggttct atgtcttccg ccagcaacca ggtcatgaag
    11941 gtgtaacgca gcaaacgctg acgctaacct ttaacagtag cggtgtgttg accaatattg
    12001 ataacaaacc tgcgctgagt ggtaactaat aataaagttg ctctcaaaga cgttaaaaaa
    12061 ggtgctcaat gagcaccttt tttctgtccg ttatttattc gctgattttt ctgctcgttg
    12121 cctgcgaagc tctttcggat cggcaatgag agggcgataa atctccaccc gatcgccatc
    12181 atgcacacta tcgcttaatt ttgccggacg gctgtaaatg ccgactttgt tttcagttaa
    12241 atcgatatcg gtacgcaatt ccagcaagcc actggcgcga atagcttctt caaccgtcgc
    12301 gccctcctgc agcgtcacgc gctgcaggta ctgcttctta ggtagcgcat aagccacctc
    12361 aacggcaatt ttacctggca ctgtaaacct ctttcgcacg aaccgtaaaa gcctggacca
    12421 tattagacgc cagctcttta aacacgcgac caaaagcgag ttcaatcaac ttattggtaa
    12481 actcaaagtc gagatgaaac tcgatacgac acgcctcctg gctcagcggc gtaaacttcc
    12541 atccaccaat caatttcttg aacggcccat ccaccagatt cataagaata ctttggttac
    12601 tggtcaactg gttgcgggta gtaaacgttt tgctgatccc agccttagag acatctaccg
    12661 ccgcagtcat ctgcccagga gtggactcca gaatccgact tccggtacaa cccggcaaaa
    12721 actgaggata agactgaacg tcattcacta actgatacat ttgctccgcg ctgtagggta
    12781 ccagtgcggt ccggctaatc tgaggcataa caatttccat caacaaaaat ccaacaaata
    12841 atatcattta tcctgcagta aaaaaaacgc tatcccggcg ctgggtaaca tccggttcat
    12901 gctaagatag agccttgtcc cccgcaggat tgatatgggg tattttcgat ttcagattac
    12961 cgatgattca cgacgcttat gacgaagaaa aaagcacata aacctggttc agcgaccatc
    13021 gcgcttaaca agcgcgcccg tcacgaatac tttattgaag aagagttcga agcgggactt
    13081 gccctgcaag gctgggaagt taaatccctg cgcgcaggaa aagccaatat tagcgacagc
    13141 tatgtccttc tgcgcgacgg agaagcgttc ctgtttggcg caaacatcac gccaatggct
    13201 gtggcttcta cgcatgtggt gtgcgatcct acccgtaccc gcaagttact tctcaaccag
    13261 cgcgaacttg attcgttgta cggtcgcgtc aatcgagaag gctataccgt agtggcgctc
    13321 tccctgtact ggaaaaatgc ctggtgcaaa gtgaaaatcg gcgtcgccaa aggtaagaaa
    13381 cagcacgata aacgttcaga tatcaaagaa cgcgaatggc aggtggataa agcgcgtatc
    13441 atgaaaaacg cccaccgtta aacctgcact ccaattcttg acctgttcct caccgcgcct
    13501 ccctctccgg cggcgcgaat gaacatctta ttggctatca catccgacaa aaatgttgcc
    13561 atcccattgc ttaatcgaat aaaaatcagg ctacatgggt gctaaatctt taacgataac
    13621 gccattgagg ctggtcatag cgctcataaa tctggtatac ttgccgttac acattggggc
    13681 tgattctgga ttcgacggga tttgcgaaac ccaaggtgca tgccgagggg cggttggcct
    13741 cgtaaaaagc cgcaaaaaat agtcgcaaac gacgaaaact acgctttagc agcttaataa
    13801 cctgcttaga gccctctctc cctagcctcc gctcttagga cggggatcaa gagaggtcaa
    13861 acccaaaaga gatcgcgtgg aagccctgcc tggggttgaa gcgttaaaac ttaatcaggc
    13921 tagtttgtta gtggcgtgtc cgtccgcagc tggcaagcga atgtaaagac tgactaagca
    13981 tgtagtaccg aggacgtagg aatttcggac gcgggttcaa ctcccgccag ctccaccact
    14041 ttaagaagga ctacaaccgg acagtagcaa taaatacagc cacttacgga cacaagccag
    14101 acagtagtcg gactgaaaaa agacaaaaat atacacgtga aatgcacgtg cacttctaaa
    14161 aaaccccagt tatcaccgac tggggttttt caatttgtaa
//
LOCUS       DAZFDC010000120        14180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFDC010000120 DAZFDC010000000
VERSION     DAZFDC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.120"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..351)
                     /locus_tag="ACHJ48_003742"
     CDS             complement(<1..351)
                     /locus_tag="ACHJ48_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709215.1"
                     /translation="MKAKYVILSVLNKQELFYLKSRYKEMKKLANKHEVSLVEQNFID
                     AIISMVGYCSLGFGLVSIVNGILLVFSFFQDNMLKSFFLSSIVSIAMFAILIIHRKLD
                     TKFSYQLCLKMFYLK"
     gene            complement(720..2465)
                     /gene="ggt"
                     /locus_tag="ACHJ48_003743"
     CDS             complement(720..2465)
                     /gene="ggt"
                     /locus_tag="ACHJ48_003743"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO4709216.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDELADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSLIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            2585..3025
                     /locus_tag="ACHJ48_003744"
     CDS             2585..3025
                     /locus_tag="ACHJ48_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO4709217.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            complement(3012..3755)
                     /gene="ugpQ"
                     /locus_tag="ACHJ48_003745"
     CDS             complement(3012..3755)
                     /gene="ugpQ"
                     /locus_tag="ACHJ48_003745"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO4709218.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(3752..4822)
                     /gene="ugpC"
                     /locus_tag="ACHJ48_003746"
     CDS             complement(3752..4822)
                     /gene="ugpC"
                     /locus_tag="ACHJ48_003746"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO4709219.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDICINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(4824..5669)
                     /gene="ugpE"
                     /locus_tag="ACHJ48_003747"
     CDS             complement(4824..5669)
                     /gene="ugpE"
                     /locus_tag="ACHJ48_003747"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO4709220.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(5666..6553)
                     /gene="ugpA"
                     /locus_tag="ACHJ48_003748"
     CDS             complement(5666..6553)
                     /gene="ugpA"
                     /locus_tag="ACHJ48_003748"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO4709221.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSRFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVMQFRYVESKVRYQ"
     gene            complement(6651..7967)
                     /gene="ugpB"
                     /locus_tag="ACHJ48_003749"
     CDS             complement(6651..7967)
                     /gene="ugpB"
                     /locus_tag="ACHJ48_003749"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO4709222.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(8327..9073)
                     /locus_tag="ACHJ48_003750"
     CDS             complement(8327..9073)
                     /locus_tag="ACHJ48_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4709223.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            complement(9192..9905)
                     /gene="livF"
                     /locus_tag="ACHJ48_003751"
     CDS             complement(9192..9905)
                     /gene="livF"
                     /locus_tag="ACHJ48_003751"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO4709224.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(9907..10674)
                     /gene="livG"
                     /locus_tag="ACHJ48_003752"
     CDS             complement(9907..10674)
                     /gene="livG"
                     /locus_tag="ACHJ48_003752"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO4709225.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(10671..11948)
                     /gene="livM"
                     /locus_tag="ACHJ48_003753"
     CDS             complement(10671..11948)
                     /gene="livM"
                     /locus_tag="ACHJ48_003753"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO4709226.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(11945..12871)
                     /gene="livH"
                     /locus_tag="ACHJ48_003754"
     CDS             complement(11945..12871)
                     /gene="livH"
                     /locus_tag="ACHJ48_003754"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO4709227.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(12919..14028)
                     /gene="livK"
                     /locus_tag="ACHJ48_003755"
     CDS             complement(12919..14028)
                     /gene="livK"
                     /locus_tag="ACHJ48_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO4709228.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         3455 a         3996 c         3528 g         3201 t
ORIGIN      
        1 tttaagataa aacattttga ggcataattg gtaagagaat ttagtatcca gttttctgtg
       61 tatgataaga atagcgaaca tggcaatact aacgattgaa gacagaaaaa aacttttcaa
      121 catattatcc tgaaaaaatg agaatacaag caatatccca ttaactatac ttaccaaccc
      181 aaaccccaaa gagcaataac ccaccattga aataatagca tcaatgaaat tttgttctac
      241 gagagagact tcatgcttat tagcaagttt cttcatttct ttgtatcttg attttaaata
      301 gaagagttct tgtttattaa gtacagagag aatcacgtat tttgctttca ttattccccc
      361 ttaaaaacac tttacaaaat cgatcaaagt catgatgaat gccattatgc acataaacaa
      421 cacaaagcgt gatatttaaa tcaataaatt acactctaaa atgaactata actacagttt
      481 atttaatagc atagttaatt cctttcctga atatcaggaa gtaattatcg cacgtcctat
      541 tttcacggta aatgaaagac tattctgcat actcttgaaa agaaagcata acctaagccc
      601 cttaaaagca ttcaggataa ttatgattat atattacgga gcaatagtaa aagcaatcca
      661 gacactaatt tatcttgttc aagaaaacag ccctcttccc acgaagaggg ccgctaacct
      721 tagtaccccg ccgttaaatc atccaccgag cgcgggtcgg atgcgccgta caactcaccg
      781 tccggcccaa ccataatgct ttgtgtactg cccatcgcct ctttcagcgc cactttctga
      841 ccttttgctt ccagcagctt gagcgtatcc gggctaaacc ctttttcgac acgcagctcg
      901 tccggcaacc actgatggtg gaaacgcggc gcattggtcg cttcggcgac gttcatgcca
      961 taatcgatgc tattcaccac catttgaagc actgtagtga tgatcaggct cccgccaggg
     1021 ctaccggtga ccagccaggt tttaccgtct ttcaccacaa tggttggtga catcgatgac
     1081 agcgggcgtt tgttcggccc gacggcgttg gcatcaccgc ccaccagccc gtaaacgttc
     1141 ggtacgcccg gtttggcaga gaaatcatcc atttggttat tgagcaaaat accgctctcg
     1201 cccgcgacaa tacccgtacc gaaggtggtg ttcagcgtat aggtcaccgc caccgcgtta
     1261 ccgtctttat ccaccaccga ataatgggta gtttgattac tctcataagg cgcaagcttg
     1321 ccggggcgaa tctcgctgga cggtttcgct ttattgatat cgatttgctc ggcaatagat
     1381 ttggcatagg ctttattggt cagcgcctgc cacgggactt tcacaaaatc cgggtcgcca
     1441 agatattccg agcggtcagc gtaggcatat ttctccgctt ctgccatgat ctgcatcgcg
     1501 tcggcgctgc caaaaccgta tttcttcata tcgaagtttt ccagaatatt gaggatttgt
     1561 acgatatgga tcccgccgga ggatggcggt ggcatggagt aaacctgata cccgcgataa
     1621 tcgccgctta tcggtgtgcg ttcgaccgct ttatacgccg ctaaatcttc tttagtgatc
     1681 aggccaccgt ttttctgcat ctcctgggca atctgttccg ctatcgtgcc tttgtagaat
     1741 tcgtccgggc cgttttcggc aatcatctcc aggctctttg ccaggttcgc ctgcaccagc
     1801 ttgtcgccct ttttcagcgg ctcgccctct ttccagaaga tagccttgct gttttcgtga
     1861 ttcggcagca cttcgctccc gtaggttttg agatcgtcag ccagctcgtc gttaacgata
     1921 aaaccatcgc gtgccagttt aaacgctggc tgcaccactt tgttcaacgg cattgtgccg
     1981 tatttatcca acgccagcga gaaaccagcg accgtaccgg gcgtgccgga agccagatgc
     2041 gaagtgagtg attttttgct gtccgggttg ccctggtcat caaggaacat atcgcgggtc
     2101 gctttggcgg gtgccatttc gcggaaatcg atagcggtgg tattgccatt tttcgagcgg
     2161 atcagcataa aaccaccacc gcccagattc cctgcctgcg gatgcgttac cgccagcgcg
     2221 tagcccacag ccacggcggc atcaacggca ttcccgccct ccttgaggat atccaccccc
     2281 acctgagtgg cagtagcatc tacagatgcc accatcccct gtttcgcgcg taccggatga
     2341 aagacatctt cctccacgcc gtaagaaaca ggagacggcg caggtggcgc ggctaccgta
     2401 ctaaaacaac ttcctgtgaa cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
     2461 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
     2521 aattaatcct gatgattgca ggaaaggagt aaacttaaag aatatcttca gaggaggata
     2581 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
     2641 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atccccagtc agcagcggat
     2701 gcaaacccag atgcaaacgc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
     2761 aacgcaaact cagttgcaac aacagcattt agaaaaccag ataaacaata attctcagcg
     2821 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
     2881 caatggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
     2941 gttgccggag aggagaaacg gcgacatgct gaatcagccc ggcacgccgc agcctgatat
     3001 accgttgaaa actattgggc cgtaaagttc ggaccaatca catcaatcgc atcggtacag
     3061 atgcaatcca cgccccagcg cagcaactct gccgcgcgct ggggtttatt gacggtataa
     3121 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagcaactta
     3181 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttctcgcca gtcgtcgcgc
     3241 cactcatcca gcaacaaacc gcgcggcagt tctggtactg cctgttgtgc ggcttctaaa
     3301 gcatcaatct caaacgatga cagtagcggc ggcgtcatac cggcccacag ctcgcgtgcc
     3361 gccagcgcca ccattttgcc cgttaatggc ccagtgccag tggtgggttt gatttcgata
     3421 ttcgccatca tcccatgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
     3481 tcacctttaa acgctttgcc gtaccaactg cctgcatcca cacgtagtaa atcctgccag
     3541 ttcagttcac ccgcgacgcc ccagccgttg ctggtacgtt cgagattgtc gtcatggagc
     3601 aggaagattt cgccatcttt cgacaacttc gcgtcaaatt cgatcatctt atgaccgtat
     3661 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagtttacc gccgccacgg
     3721 tgggcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
     3781 aaagatgtag ctgattttcc ggcagatgca gccacagcgt gctgcctgcc gtcgggcgct
     3841 cctgatgcgc cagccgtacc accagcttct gttcgcccca gcgtccgtgc gccaagttat
     3901 ctgcgccgag gatctccagc gtgtccatca ccagcggcac gccgccttct gcctgcgagc
     3961 ttagcgcgat atgttccggg cgaatgccga gagtcatttt acgccctgca tactgacggt
     4021 agccaccgtt tagcggcagc gcaatgccgc cgtccagctc gaagtgcgtg ccttcgttat
     4081 tcacgcggcc tgtcagcaga ttcatcgccg ggctgccaat aaaactcgcc acaaacaggc
     4141 tggcgggctt ttcgtagact tcaactggtg tgccaatctg ttcggcaacg ccgccgttca
     4201 tcaccattac tcgctgggcg agggtcatcg cctcaacctg atcgtgagta acgtagagtg
     4261 aagtcgtttt caggcgacgg tgcagctgtt gcagttcaag acgcatctgt acgcgcagct
     4321 tggcatcgag gttagagagt ggctcatcaa acaggaacac cgccggatcg cgcacaatcg
     4381 cccggcccat cgccacacgc tgacgctgac cgccggaaag ctcgcgcggg cggcgcttaa
     4441 gcagaccgtc cagttccaga atgcgcgccg cctctttaac gcgctcggca atttgctgct
     4501 tgcccatgcc gcgaattttc agcccccacg ccatgttttc ttcaacactc atatgcggat
     4561 aaagcgcgta gttctggaac accatcgcaa tcccgcgatc tttcggctcc atttcggtca
     4621 cccgctggtc gttgatacag atatcgcctt ccgtcacccg ctccagcccg gcaaccatgc
     4681 gcagcagcgt cgatttcccg cagcctgacg gcccgaccat cacgataaat tcgccatccg
     4741 ccacatcaag ggtcagcggt ttaatcacct gggttttgcc atcccagctt ttggttactg
     4801 cctgtaattt cagtcctgcc atcttatttc tcactatcga ccaggccgcg cacgaaggca
     4861 cgctgcatca ctaaaacaat cactactggt gggatcaggg ttaacaacat cgccgccatc
     4921 actgagttcc attctgtggt gccttcgcca gtggcgatca tccctttgat ccctgccacg
     4981 gtggtgccga gatccacgtc ggtaataatc aacaacggcc acaaatactg gttccagccg
     5041 taaataaagg tgatcacaaa caacgccgcc agattagttt tggacaacgg aaaaacgatg
     5101 tcgcaaaaga agcgcattgg cgacgcgccg tcgatccgcg cggcttccac cagttcatcc
     5161 ggtagggtca taaagaactg gcggaaaaga aacgtcgcgg tcgccgaggc catcagcggc
     5221 agcgttaaac ctgcgtagct gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg
     5281 aagatacgta cttcaaccgg caacatcagg gtgataaaaa tcatccagaa aaagaggtta
     5341 cgcagcggaa aacgaaacca gacaatggcg aatgccgaga gcatcgagac ggtaattttg
     5401 ccgagcgtaa tgctgaacgc catcacgaag ctgttaagca acatccgcca gaacggcgca
     5461 ctatttgtgc ctaccccgtt tacccagatg ttgtggatgt tttccagcag atgcgtgccg
     5521 gggatgagcg tcatcggcgc ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac
     5581 gccacgtaca gcgggaatag gatcaccgcg atcccgagga tcagcatggt atggctgaat
     5641 atcgtcagcc acggacggtt ctcaatcatt ggtaacgcac cttgctttca acatagcgga
     5701 actgcatcac cgtcagcacg atgacgagga acatcaacac caccgactgt gcggcagacg
     5761 aagccagatc cagtccggta aagccttcgc ggtagatctt ataaatcagc gtagtggtgg
     5821 cctgaaccgg cccgccggac gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat
     5881 acaccaggtt cactaccagc aggaagaaac tcaccggggc gataagcggc agcgcaatct
     5941 taaagaagcg gcgaatcggc cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg
     6001 gaatggattg cagcgcggca tagaagaaca ggaagttgta gctgatttgc ttccatactg
     6061 aggcaaacac caccagaaac attgcctgac cgctgttttg cgcgtggttc cagtcgtagc
     6121 cgaactcagc gagaaaatgg gtgatcagcc cgcgaccggg gttaaacagg aagatccaca
     6181 atacggcggc aacggcggga gccacggcat acggcagcag catcaaggtt tgatagaaac
     6241 gactgccgcg cacgatgtac tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc
     6301 tgacggtgac aaaggtgctg aattttatcg tcgtccagaa ggagtcgata tagtagctgt
     6361 catgaaacag cgcgacgaag ttatccaggc cgacaaaccg gctggagaaa ccaaacggat
     6421 cgacgctttg cagcgagtac cacaacgctt cgcccgcagg ccagataaaa aagataacgg
     6481 tgatgatgag ctgcggcgcg accagcagat aaggcagcca gcgcgagcgg aacaccggac
     6541 gggatgatga cattacggtt aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc
     6601 tacacgcgat gccaggtcag ataagcgaaa cgcatccgac attacactga ttaagacttc
     6661 gtcgatttct caaagcggcg cagcaactga tttccacgct caacggcggt atccagtgcc
     6721 tgctgtggtg tcttcttacc ggtccacacg ctctccagct cttcatccac aatcacgcgg
     6781 atctgcggca tgttgcccag acgcagccct ttggtgaacg gcaacggcgg cttattcagc
     6841 atctgacgcg tcgcggtatc cgcccctggg tttttctcat aaaagccctg ctcacgggtc
     6901 aggtcatacg ctgctttggt gattggcaga taaccggttt tctgatgcca ctcggcagcg
     6961 ttttctggct tcgcgaggaa gtcgaggaac ttcgccacgc cggtatacgt ttctttatct
     7021 ttgccctgca tcacccacag gctggccccg ccgataatgg cgttttgcgg tgcatctttc
     7081 gcatcggcgt cgtaaggcat catgcctacg ccatagttga atttggcgta ctcgcgaata
     7141 ttagcgagag aaccggaaga ggcggtggtc atagcgcaat caccgttata gaacttctcg
     7201 gtggattcat ccttacgccc gacgtagctg aaatcgccct tcttgttcat ctcttcgagc
     7261 atggcgatgt gtttcacctg ctccggcttg ttgaactcca gcaccgcatc cgtgccgtca
     7321 aagccgttgt ttttgctggc aaacggcaga ccgttccagg cgctaaagtt ttccagttgg
     7381 atccagccct gccagccgct ggcgtagccg cacttcatgc cggaggcttt tagttttgcg
     7441 gcatagtccg ccagatcctg ccaggttttc ggcggttgtt ccgggtctaa ccccgctttc
     7501 ttgaaggcgt ctttgttgta atagagaacg ggagtcgagc tgttgaatgg ctgggagagt
     7561 aagtggcccg ttttgctgtc ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca
     7621 tcgaattgaa tccccgcctc tttaaacacg tcataaaccg gtttaatggc tttcgacgcc
     7681 atcatggtgg cggtgccaac ttcataaacc tgcaaaatag ccggagcatt gccggtacga
     7741 aatgcggcaa tccccgcgct taaattctgt tcgtaattgc ctttataggt cggtacaatt
     7801 ttgtaatccg ggttttcggc gttaaaacgt tgggccagag aatccacctc tttacccagt
     7861 tccccttcca tagaatgcca gaacggaatg gtcgtcactg cctgtgcatt ccccattaac
     7921 gccagtccga gcgccagtgc tgaagctgta taatgtaacg gtttcatcgt ttatctctct
     7981 tgttgtaccg aatgcgcgaa ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg
     8041 aattacagaa aaataacttt tttgttacat ttgtaagata gtaaggtgtc agaaagatga
     8101 caaggcggtg acggtacggg tgagggaaaa tgggagatgg ggcacggata tcccgagaat
     8161 gcactgaaaa gaggcgatta acggccagct aatcgccaga actccttacc atgaagagca
     8221 aaaatggcag caaattctgc gtccaatttt gctgcccgat gagagctaca tttttcaatt
     8281 ttaacaaaag attgccgcga cactggtcta acggcaaaga agattttcat tgcaccacac
     8341 tctgcttcag ccgctcattg gcggtcttaa tccattcccg gtggtgtttt ctataatgag
     8401 gtcttgagcg tttagccgta tcaaccacgg caacatactg ctcgttggct tcacgtaacc
     8461 gactcatttt cgccaacatt tccgcatagt aaatacgcgc ctgcgggccg gggtaataag
     8521 aaatcaatac ttcaaactcg ctttccgcat cagcgtattt ttcttgtgcg gctaacgttc
     8581 tggcaaataa caaatgcccg tctgccgact gaaaatcagg gttataacgc atcacatctt
     8641 ccagcgtctg ctgacaggcg gcgaactcct gaatagcaaa ctgtgcctgc gccaggctta
     8701 gcatcattgc cgcttcatgg gcgaatatcc cgcttaacgc ctgctgataa tggggaacag
     8761 cttcgtgata acgccccaga cgcgccagtt cattagccaa cgcataatgg ttttgattcg
     8821 tctcggcaat cgccagttct tcggttaatt tgcgcaggtg gcgctcaggg ttgagtttgt
     8881 cctgcaggcg catcagcaaa gtatgcccgt gacggtcagc gcccgtttca ggcagcatca
     8941 ctatgacgaa atacgccacg cagccaatca ccggaaaaca gaaaatgata aaaatccagt
     9001 accgctcttg tccggtacgg taagcgtgaa tacagcaaag cagttcaagc atgacaacaa
     9061 cggcataatg catgggaggg aacctttccg tggcaaataa gtggtctggt tttaatcctc
     9121 ccggtcagtt gaaacaagtc tgtatacgga cgaataaccc ggcagaactt agctgccggg
     9181 ttatcgataa tttacccgcc taaatacgca cttctcactg cttcgttcgc cagcagcgca
     9241 tcaccagtat cggagagcac aacatggccg ttttccagca cgtagccgcg atcagcgagc
     9301 ttcagcgcct ggttggcgtt ctgctcgacg aggaagatag tcatcccctg ctcgcgcagc
     9361 tgctcgatgg tgtcgaaaat ttgctggatg ataatcggcg caagaccgag cgatggctca
     9421 tcaagcaaca acaaacgcgg attgctcatc agcgcgcgac caatcgccag catctgctgt
     9481 tcaccgccgg acatggtgcc tgcccgctga acacgacgct catgaagacg tggaaacagc
     9541 tcatacaccc actttatgcg ctcctgaaac tgatcacgtt cggcaaaaaa gccgcccatc
     9601 gccaggttct cttccaccgt catccgcgag aagacgcgac gcccttccgg gacaatcgct
     9661 accgcttcgc gcatgatttt cgccgtctgc cagtcggtaa tgtctttatc atcaaacaca
     9721 attcgcccgc tggtggcgcg cggatcgccg cataacgtgc cgagcaaggt ggttttcccc
     9781 gccccgttcg cgccaatcag cgtgacaatc tcgccctgat tgatatgcag gctcacctca
     9841 tgcagcgcct ggattttgcc gtagtgggcg ctgactttgt caaaggacaa catgactttt
     9901 tccatcttat gcctcaccta aataggcacg gatcacgtcc gggttattac ggatttgctc
     9961 cggcgtaccg tttgccagcg gcgtcccctg attgaccacg taaattcggt ccgaaattcc
    10021 catcaccagc ttcatatcgt gttcaatcaa caagatagtg gtgttgtgat gattgcgcag
    10081 ttcggcaatc agctcatcca gctctttcgt ttctttcggg ttaagacctg ccgcaggttc
    10141 gtcgagcatt aaaatctccg gctgcgtcac catgcagcgg gcaatctcca gacggcgctg
    10201 gtcaccgtag gccaggttac tcgcctgacg gttggcgtgt tccagcaaac caatgcgctc
    10261 aagccaggtg gcggcgcggt cgagcgcttc actttgggcg cgacggaaag aaggcgtttt
    10321 caacaggcca gagaacagcc cggttttcag ttgctgatgc tgcgccacca gcaggttttc
    10381 aattaccgtc atttcgcgga acagacgcac atgctggaag gtacgcacca cgcccatgcg
    10441 ggcgatttgc tgccccggca aaccctccag atgctgatcg cgcagtaaaa tagtgccgcc
    10501 ggtgggtttg tagaatccgg tcaggcagtt aaagaccgtg gtttttccgg caccgttagg
    10561 accgattaac gagacgatct cctgcgggta caattcaaga ttgacgttat tcacagccag
    10621 caggccgccg aagcgcatca tcaggccgtt aacagataat aatggctgac tcatgcctgc
    10681 tctcctttcg ctgcgccgtt tttcagcttc agttgcgggc gcgtcatggg cagcaagccc
    10741 tgcggacgcc agatcatcat cagcaccatc aaaccaccga gcattaacat gctgtattcg
    10801 ttgaaatcac gcatcaactc gcgcgacacc accagcaaaa ttgccgccag aattaccgca
    10861 aattgcgagc ccataccgcc gagcaccact atcgccagca caaacgccga ttcggcaaag
    10921 gtgaaggatt ccgggctgac aaagccctga cgcgcagcaa acagcgttcc ggcaaaaccg
    10981 gcaaacgcgg cgctgatggt aaaggcagtc agcttgatac gacgcgggct taagcccagc
    11041 gaacggcagg cgatttcatc ttcacgcaac gcttcccagg cgcgacccag cggcatccgc
    11101 agcaggcggt taatgacaaa caggcttagc accaccagca gcaacgccac caggtagagg
    11161 aaaatgacac gatcggaggg atcgtatttc aggccaaaga aattactgaa cgtgtcccag
    11221 ccgccttcac gagcggtacg gctgaactca aggccgaaaa aagtcggttt cgggatctga
    11281 ctgataccgt tcggaccgcc ggtaatttcg gtgttattga gtagcaatat gcgcacaatt
    11341 tcgccgaaac cgagggtaac gatcgccaga tagtcaccgc gcaagcgaag caccgggaag
    11401 ccgagcagga agcccgccgc cgccgccatc aacccggcta tcggcagaca ggtccagaag
    11461 cccaagccgt aatagtgatt gagcagcgca aaggtataag cgccgatggc gtaaaagccg
    11521 ccgtacccca gcaccagcag accagaaagc cctaccacca cgttcagccc gagtccgagg
    11581 ataatgtaga tcatggtcag ggtggcgata tccaccgtcc cgcgtgagac cataaacggc
    11641 cacgccaccg caagcaccag cagcgccacg agaaacagct tttgcttcac cgtggagcca
    11701 tcaatggcgg gcagaataaa cttcggtccg gaaacgcttt tcaacccttt ctggaaagcc
    11761 ggtcgcaaaa gctggaagaa aaagaccacc gccgtgccga taaacaccca ctgccaacgg
    11821 atgtctgaag ccgtgtcgac caccagtttg gtgccatcca gctccagttg tacgcccata
    11881 aagacgcccg ccaggacaaa aaacatcgcg gcagagagca acgccattgc aatatgcatc
    11941 ggtttcatac tttctctacc tccgggcgac ccagaatacc ggtcggcatc accagcagca
    12001 ccagaatcag cagggcgaat gagaccacat ctttatattc cgtactcagg taggcagaag
    12061 agagcgcctc cgcaatcccc agaatcagcc cgccaatcat cgctcccgga atgctgccaa
    12121 tcccaccgag caccgcagcg gtaaaggctt tcatcccggc cataaagccg atataggggt
    12181 taatgacgcc gtagaactga ccgagcagta cgcccgccac cgccgccatc gccgcgccaa
    12241 tcacaaaggt caaagcaatc acccggtcgg tgttaatgcc aagcaggctc gccattttca
    12301 gatcttcggc gcaggcacgg caggcgcgcc ccatacggga ataacgaatg aaaatcgtca
    12361 gcgccagcat ggcgaggaag gtgacaatcc agatcaccgc ctgcatggtg gtaatagagg
    12421 cagagaagtt ttcgctatga cccactaccc actgaccatt aaacaagctg ggcagcgcca
    12481 cgtcgcgcga accttcagtc aggctgacgt agttttgcag gaagatggac ataccgattg
    12541 ccgagatgag tgcaatcagg cgcttagagt tacgcaccgg gcggtaagcc acccgttcga
    12601 tactccagcc gtaggcgctg gcaatgacga ttgcgccgac gaatcccgcc gctaccagca
    12661 gccagccggt atcaatgccc atcatcatca gtgcggcgat gatcataaat gagacgtagc
    12721 tgccgatcat gtaaacctcg ccgtgggcga agttgatcat gccgataatg ccgtaaacca
    12781 tggtgtagcc gatggcaatc agcgcgtaag tactgcccag cgtgacgccg ttaaacatct
    12841 gctgcaagaa atacaaaaac tgctcagaca taaggtaacc tttctaaacc cgcccgcatt
    12901 tcacgggcgg tgggattttc acttggctgc cgtggatgaa ccgtcggcgt gccactggaa
    12961 gacaccaaaa tcaaatccct taagatcgcc tttttcatcc cagttcagcg gcccaatcac
    13021 ggtgtttgca ccgttagctt ttaaatcttt caccagcgcc agcggctcat cgctgccggt
    13081 acgttcaagg gcagttgcca gagattgcac cgccgcgtag gtgatccaga cataaggccc
    13141 ggacggatct ttcttgtctg ctttcagcgc atcaacgatg ccctggtttg ccggatcctg
    13201 gtcatagcgt tttggcatag tgaccaacat gccttcggcg gcatcaccgg caatgttcga
    13261 caacgacgca ttacccacac cttccggccc cataaactgg gttttcaggc caacggaacg
    13321 ggcctggcgc agcatctgcc ccatttccgg gtagtaaccg ccgtagtaaa cgaagtcgat
    13381 gttttctttt ttcaggcggg cgatcagcgc ggagaaatct ttctccccgg cggtaatacc
    13441 gtcgaagaag acgacgttgg cgttagccgc tttcagcccg tcctgcaccg aacgcgccag
    13501 cccttcgcca tactgttgtt tgtcgtgaat gatggcgatg cgctggggct tcaccgtctc
    13561 aagaatgtat tttgccgccg ttggcccctg ggaagagtcc agcccggcag tacgcataat
    13621 gtgttgataa ccgcgttggg tcagctccgg gtttgtcgct cccggcgaga tcataagaat
    13681 accttcgtct tcatagatat ctgacgcagg ctgggtagaa gaagaacaca gatgaccaat
    13741 aacgtattta atgccgtcat taacgatttt gttggcgacc gcaacggctt gtttcgggtc
    13801 gcaggcgtcg tcatattcta cgccaaccag cttatcgcct ttaattcccc ctttggcatt
    13861 aatgtcttta atcgcctgac gcgcgccgtt aaattccata tcgccccact gggcaatcgg
    13921 gccggacatc gcgccgacaa cggcaacttt aatatcgtcg gctaaagcgg tgtgtgaaat
    13981 tgccagtgca atcatccctg cgatgatagt tttcgcattc cgtttcatag tcaaaaatcc
    14041 ccattcgtga tgttgtgttg ctttgttttt atgtgttaac aaatcagact gttctttttt
    14101 tatactgcac tgtttttgcc tgtctgatta agggtttagc gcagtatttt gtgataatag
    14161 cgattaaagt ccctattttt
//
LOCUS       DAZFDC010000121        14161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFDC010000121 DAZFDC010000000
VERSION     DAZFDC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.121"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..96
                     /locus_tag="ACHJ48_003756"
     CDS             <1..96
                     /locus_tag="ACHJ48_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709229.1"
                     /translation="KVFKAGLRRKMRKAAMDRNYLASVFAGSGLS"
     gene            362..2599
                     /gene="nrfB"
                     /locus_tag="ACHJ48_003757"
     CDS             362..2599
                     /gene="nrfB"
                     /locus_tag="ACHJ48_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO4709230.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2586..5558
                     /gene="nfrA"
                     /locus_tag="ACHJ48_003758"
     CDS             2586..5558
                     /gene="nfrA"
                     /locus_tag="ACHJ48_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO4709231.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSITTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQESANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAARQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRFFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5559..6449
                     /gene="ybcH"
                     /locus_tag="ACHJ48_003759"
     CDS             5559..6449
                     /gene="ybcH"
                     /locus_tag="ACHJ48_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO4709232.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYFSAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6632..7393
                     /gene="envY"
                     /locus_tag="ACHJ48_003760"
     CDS             6632..7393
                     /gene="envY"
                     /locus_tag="ACHJ48_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO4709233.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7439..7515)
                     /locus_tag="ACHJ48_003761"
     tRNA            complement(7439..7515)
                     /locus_tag="ACHJ48_003761"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7479..7481),aa:Arg,seq:tct)
     gene            7758..8390
                     /gene="fimZ"
                     /locus_tag="ACHJ48_003762"
     CDS             7758..8390
                     /gene="fimZ"
                     /locus_tag="ACHJ48_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO4709234.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8393..8908)
                     /gene="sfmF"
                     /locus_tag="ACHJ48_003763"
     CDS             complement(8393..8908)
                     /gene="sfmF"
                     /locus_tag="ACHJ48_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO4709235.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALLPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(8919..9926)
                     /gene="fimH"
                     /locus_tag="ACHJ48_003764"
     CDS             complement(8919..9926)
                     /gene="fimH"
                     /locus_tag="ACHJ48_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4709236.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(9939..12548)
                     /gene="sfmD"
                     /locus_tag="ACHJ48_003765"
     CDS             complement(9939..12548)
                     /gene="sfmD"
                     /locus_tag="ACHJ48_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO4709237.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(12579..13271)
                     /gene="fimC"
                     /locus_tag="ACHJ48_003766"
     CDS             complement(12579..13271)
                     /gene="fimC"
                     /locus_tag="ACHJ48_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4709238.1"
                     /translation="MMTKIKLLMLIIFYLIISASVHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(13491..14033)
                     /gene="fimA"
                     /locus_tag="ACHJ48_003767"
     CDS             complement(13491..14033)
                     /gene="fimA"
                     /locus_tag="ACHJ48_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4709239.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         3730 a         3538 c         3363 g         3530 t
ORIGIN      
        1 aaggtattca aggcagggtt aagacgtaag atgcgaaaag cagccatgga cagaaactat
       61 ctggcgtcag tctttgcggg gagcgggctt tcgtaatctt gccctgcctg ctcacctcca
      121 ggcacttcca ctacatcatg acctgatcgg tgccaccact cctcttcact atgacgttaa
      181 cctcccctcc ttgacaccac gcttttgtct acattctacg caaacggcaa cttttataac
      241 cagtgtaaaa ataacgtgcc gcaatagtca tacctattaa tggtaaaaag ctgtcacaat
      301 tcataaaaaa ccttaatata cgccacccta aacataacca gcgttaatgt aaggtttttg
      361 tgtggactgg cttcttgatg tttttgctac ctggctctat ggcttaaaag taatcgcgat
      421 aacgttagcg gtcatcatgt tcatcagcgg gctggacgat ttttttattg atgtcgtcta
      481 ctgggtacgc cgcattaaac gcaagttgag tgtttatcgc cgctacccgc gaatgagtta
      541 ccgcgaactg tataaaccag atgaaaaacc gttagcgatt atggttccgg cgtggaatga
      601 aacgggcgtc atcggcaata tggccgagct ggcggcgacc acgctcgact acgaaaacta
      661 tcatatcttt gttggcacct accccaacga cccggacact cagcgtgatg ttgacgaagt
      721 gtgcgctcgc ttcccgaatg tgcataaggt agtctgcgcg cgtcctggcc ccaccagcaa
      781 agccgactgt ctgaacaacg tgctggacgc catcacccag tttgagcgta gcgccaattt
      841 cgcttttgct ggttttattc tgcatgacgc cgaagatgtg atttcaccga tggaattgcg
      901 tctgttcaac tatctggtcg agcgtaaaga tctgattcag atcccggtgt atccgtttga
      961 acgcgaatgg acgcacttca ccagcatgac ttacattgat gagttttcag agctgcatgg
     1021 caaagatgtt ccggtgcgtg aagccctcgc cggacaagtg cccagcgcag gcgtcggcac
     1081 ctgtttcagc cgccgcgccg tgaccgctct gttagctgac ggtgacggta ttgctttcga
     1141 cgtgcagagt ctgactgaag attacgacat tggcttccgc ctgaaagaaa aaggtatgac
     1201 ggaaattttt gtccgttttc cggtggtgga cgaagccaaa gaacgcgagc agcgtaaatt
     1261 tttacagcac gcacggacgt caaacatgat ctgcgtgcgc gaatatttcc ccgatacctt
     1321 ttcgactgcg gttcgacaaa aatctcgctg gatcatcggc attgttttcc agggctttaa
     1381 aacccataaa tggacctcca gcctgacgct gaactacttt ctctggcgcg accgcaaagg
     1441 ggcaatcagt aactttgtca gcttcctcgc aatgctggtg atgatccagc ttttgctgtt
     1501 gctggcgtat gaaagtttgt ggcccgatgc ctggcatttc ctttctattt ttagcggcag
     1561 cgcatggtta atgaccctgc tgtggctaaa ctttggcttg atggttaacc gcatcgtgca
     1621 gcgggtgatt ttcgtcactg gctactacgg cctgacgcag gggctactat ctgtcctgcg
     1681 tcttttctgg ggcaacctga ttaactttat ggccaactgg cgcgcgttaa aacaggtact
     1741 tcaacacggc gatccacgtc gcgtcgcgtg ggataaaaca acgcatgact tccccagcgt
     1801 cactggcgat acccgctcgt tgcgcccgtt aggtcaaatt ctgctggaaa atcaggtcat
     1861 cactgaagaa cagctcgata cagcactgcg taatcgcgtc gaaggtctac gcctgggcgg
     1921 ttcaatgctg atgcaggggc tgattagcgc cgagcagctg gcacaggcgc tggcagagca
     1981 aaacggcgtg gcgtgggaat ccatcgatgc ctggcagatc ccgtcctcgc tgattgccga
     2041 aatgccggcc tccgtggcgc tgcattatgc ggtactgccg ctgcgtctgg aaaatgatga
     2101 gttaattgtc ggcagtgaag atggtattga cccggtttcg ctggcggccc tgacgcgtaa
     2161 agtcggacgc aaagtgcgtt atgtcattgt tctgcgggga caaattgtca cggggttacg
     2221 tcactggtat gcacgccgac gcggtcacga tccgcgggca atgttgtaca atgcggttca
     2281 gcatcagtgg ctcacggaac agcaggccgg tgaaatctgg cggcaatatg tgccgcatca
     2341 gttcctgttc gccgaaatac tgaccacgct cggtcatatt aatcgttcag caattaacgt
     2401 gttgttattg cgccatgaac gcagttctct gccgctcggc aagtttttgg tcaccgaagg
     2461 cgttatcagc caggaaacgt tggatcgcgt cctgacaatt caacgcgaat tacaagtttc
     2521 gatgcaatca ctattactca aagcaggttt aaacacagaa caggttgcgc aactggagtc
     2581 cgaaaatgaa ggagaataac cttaatcgcg tcatcggatg gtctggttta ctgctgacgt
     2641 ctttattgag taccagcgca ctcgcagaca atatcggcac cagcgcagaa gagctggggc
     2701 tgagcgatta tcgccatttt gttatttatc cccgtctcga taaggcgctg aaggcacaga
     2761 aaaataacga cgaagcaacc gccatccgcg aatttgaata tatacaccag caggtgccgg
     2821 ataatattcc gctgacttta taccttgcgg aagcctatcg ccattttggt catgatgacc
     2881 gggcacggct gttgcttgag gatcaactga aacgtcaccc aggagatgcc cgacttgagc
     2941 gcagtctggc ggctattccg gttgaagtga aaagcattac aactgttgaa gaactgcttg
     3001 cccagcaaaa agcgtgcgat gctgcgccga ccctgcgttg tcgcagtgaa gtcgggcaga
     3061 atgccctgcg gctggcacag ttacctgtcg ccagagcgca actgaacgac gcgacgtttg
     3121 ctgcatcgcc ggaaggaaaa acgctgcgaa ccgatctgct gcaacgggca atctacctga
     3181 aacaatggtc ccaggcagat acgctataca atgaagcacg ccagcagaac acattaagcg
     3241 cggcagaacg ccgtcagtgg tttgacgtgc ttcttgccgg gcagctggac gatcggatcc
     3301 tggcactgca atcacagggg atcttcaccg atcctcagtc atatattact tacgcgaccg
     3361 cgctggctta tcgtggcgaa aaagcacgcc tccagcatta tctcattgaa aataagccgc
     3421 tgtttaccac ggacgcacaa gagaaaagtt ggctctatct gttatctaaa tacagcgcca
     3481 accccgttca ggcattggcg aattatacgg tgcagtttgc cgataaccgc cagtatgttg
     3541 ttggcgcgac gctaccggtg ctgttaaaag aaggtcagta cgacgcagcg caaaaactgc
     3601 tcgccaccct ccccgccaat gaaatgcttg aggagcgtta tgctgtcagc gtggcgaccc
     3661 gtaacaaggc tgaagctctg cgtctggcac gcttgctgta tcagcaagaa tcggcaaatc
     3721 ttacccgcct ggatcaacta acctggcaac tgatgcagaa cgagcagtca cgggaagctg
     3781 ccgatttatt gctgcaacgc tatcctttcc agggcgatgc gcgtatcagc cagactttaa
     3841 tggcgcgact ggcgtctctg ctggaaagtc atccttacct ggcaacgccg gcgaaggtgg
     3901 cgattttatc gaaaccctta ccgctggcgg agcaacgtca gtggcaaagt cagttgccgg
     3961 gtattgcaga taattgcccg gcaatagttc gcttgctggg cgatatgtcg ccttcctacg
     4021 atgccgccgc ctggaaccgt ctggcaaagt gttatcggga cacgctaccc ggtgtggcgt
     4081 tgtatgcatg gcttcaggcc gaacaacgac aaccgaacgc ctggcaacat cgtgcggtag
     4141 cctatcaggc gtatcaggtt gaggactacg ccaccgcact ggcggccagg cagaaaatca
     4201 gtcttcacga catgagcaat gaggatctgc ttgctgctgc caataccgcc caggcggcag
     4261 gaaatggtgc ggctcgcgat cgctggctac aacaggcaga acaacgtgga ctgggaagca
     4321 atgccctcta ctggtggctg catgcgcaac gttacattcc tggtcagccg gaactcgcac
     4381 tgaacgatct cacgcgctca atcaatattg cgccttctgc caacgcttac gttgcgcggg
     4441 cgacaattta tcgccaacgt cataatgtcc cggccgcggt gagtgatttg cgcgccgcgc
     4501 tggaactgga accgaataat agcaacaccc aggcagcgct tggttacgcc ttgtgggata
     4561 gcggtgatat cgcacagtcg cgggaaatgc tcgaacaggc gcataaaggg ctaccggacg
     4621 atccggcact gatccgacaa ctggcctatg tgaaccagcg tctggatgac atgcctgcga
     4681 cgcagcacta cgcccggctg gtgattgatg acattgataa tcaggcgctg ataaccccac
     4741 tgaccccaga gcaaaatcag caacgcttca atttccgccg tctgcatgag gaggtcggtc
     4801 gccgctggac gttcagtttc gattcttcca tcggcttgcg ttccggggca atgagtaccg
     4861 ctaacaataa tgttggcggc gcagcgccag ggaaaagcta tcgtagctac gggcaactgg
     4921 aagccgaata ccgcatcgga cgcaatatgc tgctggaagg cgacctgctc tcagtttata
     4981 gccgcttctt tgccgatacc ggagaaaacg gggtgatgat gccggtgaaa aatccgatgt
     5041 ccggcaccgg tctgcgctgg aagccgctgc gcgatcagat ctttttcctc gccgtcgaac
     5101 agcagttgcc gctgaacggg caaaatggcg catccgatac catgctgcgc gccagcgcct
     5161 cattctttaa tggcggcaaa tacagcgacg aatggcaccc gaacggttca ggctggtttg
     5221 cccaaaacct gtacctcgat gcggcgcaat atatccgcca ggatattcag gcgtggacgg
     5281 cagattatcg cgtcagctgg catcagaagg tagctaacgg acagactatt gagccttacg
     5341 ctcacgttca ggacaacggc tatcgtgata aaggcactca gggcgcgcag cttggcggtg
     5401 tcggggtccg ctggaatatc tggaccggcg agacgcacta cgacgcctgg ccgcacaaag
     5461 tcagtctcgg cgtcgagtat caacatacct ttaaggcgat taatcaacgt aacggagagc
     5521 gcaacaacgc gtttctcacc attggagtgc actggtaaat gcgtaagttc attttcgtat
     5581 tgctgacact gcttttggtc agcccttttt cctttgcgat gaaaggtatt atctggcaac
     5641 cacaaaaccg tgatagtcag gttaccgata cccagtggca ggggctgatg agtcagttac
     5701 gtttgcaagg cttcgatacc ctggttttgc aatggacccg ttacggcgat gcattcaccc
     5761 agccagaaca gcgcgcgtta ttgtttaagc gggccgcagc tgcgcaacag gctggcctga
     5821 agctgattgt cgggctgaac gccgatccgg aattttttat gcaccagaaa cagtcatccg
     5881 cggcgctgga aagctatctt aatcgcctgc tggctgccga tctccagcaa gccagattat
     5941 ggagtgccgt acctggcgta acgccggatg gctggtactt cagcgctgaa attgacgacc
     6001 tgaactggcg cagcgaagcc gcccgtcagc ctttgctaac atggttaaac aacgcgcagc
     6061 ggctgattag cgatgtttca gcaaaaccgg tttatatcag tagttttttc gccggaaaca
     6121 tgtcgccaga tggctatcgc caactgctgg aacacgttaa agcaaccggc gttaatgtct
     6181 gggtacagga tggcagcggc gtggataaac ttaccgctga acagcgtgaa cgttatctac
     6241 aggccagcgc cgattgccaa agctccgccc cagcaagcgg cattgtttat gaactgtttg
     6301 tcgccggcaa aggcaaaacc tttacagcga aaccgaaacc ggacgcagaa attgcctcgc
     6361 tgttagcgaa acgttcctct tgcggtaaag acactctcta tttctctctg cgctatttgc
     6421 ccgtcgcgca cggcattctc gagtattaaa tctcctccag gtaagtcggg tacgacctgg
     6481 cttacctctt tcgctctcgt cagaattcca tcaagattaa ttgctaaaaa gcgtgctaaa
     6541 gaaagaagat cgttatcggc gaacgggccg atcactcatg caggaagcat agatcctttg
     6601 agcggagttt ctgatatctg aggagtgcga aatgcaattg agcagcagtg aaccttgcgt
     6661 ggtgatcctg accgaaaaag aggtagaggt aagcgtcaat aaccatgcta cgtttaccct
     6721 tccgaaaaac tacctggccg ccttcgcgtg caacaataac gtcattgaac tctcaacgtt
     6781 aaatcacgta ttaatcaccc acatcaaccg taacatcatc aacgattatc tgttgttttt
     6841 aaataagaac ttaacctgtg taaagccctg gtcgcggctg gctaccccgg ttatcgcttg
     6901 tcatagccgt acaccggaag tgttccggct agccgccaac cacagcaagc agcaacccag
     6961 caaaccctgc gaagcggagt tgacgcgcgc attgcttttt accgtattgt ctaactttct
     7021 tgagcaatcg cggtttattg ccctactgat gtatatctta cgcagcagcg tccgcgacag
     7081 cgtctgccgc attattcaaa gcgatattca gcattactgg aatctgcgaa ttgtcgccag
     7141 ttcgctatgt ttaagcccca gcctgctcaa aaagaaatta aaaaacgaaa ataccagcta
     7201 tagccagatt gtcacagagt gtcgtatgcg ttacgccgta cagatgttat tgatggataa
     7261 caaaaatatc actcaggtgg cgcaattatg tggctatagc agcacgtcgt actttatctc
     7321 tgtttttaag gcgttttacg gcctgacacc gttgaattat ctcgccaaac agcgacaaaa
     7381 agtgatgtgg tgaagggcaa agcggaaacg gataagacgg gcataaataa agaagaaatg
     7441 gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg ctctatccaa
     7501 ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac
     7561 ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt
     7621 ttctgattta tttcgctata gcggcaaaca aacgcacacc gctgcgcgtc tgaatcaaga
     7681 aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa ttgtccgctc
     7741 ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact catcctatca
     7801 tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt gtcctgaaaa
     7861 cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat ttaatcatta
     7921 tggatataga cttgcccgga acagacggtt ttaccttcct gaaaaggatc aaacaaatcc
     7981 agagcacagt gaaagtgtta tttttatcat cgaaatcaga atgcttttat gctggcagag
     8041 cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat gatatttttc
     8101 atgccgttca gatgatcctc tccggataca cgttttttcc cagcgaaacg cttaactata
     8161 taaaaagcaa taaatgtagt acgaatagtt caacggtcac tgtgctatct aatcgtgaag
     8221 tgaccatatt acgttatcta gttagcggat tatctaataa agaaattgcc gataagttat
     8281 tacttagcaa taaaacagtt agtgcgcata aatctaatat ttatggcaag ctaggtttgc
     8341 attcaattgt agagcttatc gactacgcca aattatacga attaatataa tattaattat
     8401 aattgatcat aaatatcgca tccgctttcg ccacacctgg ccgaacaccg ctggctaacg
     8461 ctcgataatt ggcaaaaaag tttagtgtga cattgccatt tgcatctact tcctcagtcg
     8521 ggctcgcctc ccccagtgcg agccgggagc gatcgctatt acgtaattcg atggcgacgg
     8581 tttgtgccat tgcgggatca tccagagcca gcaggttggc atcagatgcc ggcgttcccg
     8641 taaataaaat cgcaactgaa cccggaggac atccctccag ccgcaggcta aaagggagga
     8701 gtgccgtggt atcgccagcg ttcagtaatt gtgtcgtagg ccatctgcct aaatctaccg
     8761 tcttatcaat atccgctgtg tttacggtac aggagaaatc aacaacgtta ccgtgcaaat
     8821 tgatattaat cgttcctaaa gggtcagctg cccatccact ggaactccac agtaacccgc
     8881 agaaacagct aaagaatact cttctcattc tccgtacctc attgataatc gacgcgtaaa
     8941 taccccagcg cgctaaacgg cccttcggtc ggtttttgac cggtaatact gataggccag
     9001 gcgcgaagtg tgacattggc tgccgcagct gcatccagac ggaaaggaat aacgctattg
     9061 agatcgttag gcgtgatcgg cgtatcgttc tgatcggcga caataaaacc taaatcctga
     9121 ttgtccgaca ccatcgcctg accagaaacg gcactggctt ccagacgcat tgttaaataa
     9181 gcctgcgcag caacattcgt acatttgacc gcaatgctct tggtttgcgg catgacacca
     9241 gcaggccgat tacccggccc tgccgcacta aataacgatg cgccgatatc accaaaatca
     9301 aattcaacaa tctgcccggc atttaattcg cagttttgcg gtacttcaac ccggccacca
     9361 aaactaatgg tataaacagg tgtaaccaac ggatcgtaag gcgttgatgt cacatacacg
     9421 gtaaacatca cctgtctggg gatctccacc atgttaataa agggccgaat aacctttaaa
     9481 cgaaatatca aacgagaatc catcaccccg aacggaaggt tttgcgaaac gttaggatct
     9541 gttcccatac gaatataatt ttgcggggga aattgaatat ccatcacact atcaaccaga
     9601 ctcatcgcac ctaatagata atcatttaat tgcatatatt tataattatc gatagtttcc
     9661 tgaacaataa agttagtgac ataactacgg tatgtataat tcaccagcgt gccgggtgga
     9721 caaattgctg agacacctac ccagccggat ttttccggta aaacaacaat ctgccctggc
     9781 tggttattgg tgctattaaa gacatttgag agatcatagt gttcatcttc tgctacgcca
     9841 gtactatttg cacaaacagt agcccaggat atgtttgcca gacacaaaca tgccatcgcc
     9901 aataataatc tgcagattat cttcattgat aatttccttt atgagccagg atgtgtgcaa
     9961 actgccgaaa taacagtgac ggcttgctgt aagctttgct tcggcaatac atagtgcgca
    10021 atgcagcgtg agttcgcgcc gtctccccac tgaaccagta attcaccaga caatggcgca
    10081 ccacttaaat aaacttgccc gtcatcaccc accatactgg ttattccggt actgttttcc
    10141 cgtaccagtg atccaaacgg caccggtttt ccgccctggg taacggtaat gagcgcccgc
    10201 acgcctatgc gggtatcaaa attggcacga accaacgcgc cttgcgtcgg cacaacgctg
    10261 ctaatatttt tttcaacatc gatggaattc cccatcgtat tggtatcaag cgcgatacgg
    10321 ttataccgat aaaccgtggc atacggcatc accgcatagc cgcgccagtc ggttaaaatg
    10381 ccagtttgat tttcaatgcg tacaccgcct gcgccaggcg ctttaatcaa aacattggta
    10441 tcccctaaag gctggctcag cgttatgcca ttttcatgtc cgaccacacc gccagacagc
    10501 tgccagttaa cgtcatgttg atcgcggtcg tagttatacc cgacccccag cgtaccgtaa
    10561 gcggcctgcc agtttgccgt ggcgctgccc gtatacccat tattcgaggt atcaccctgg
    10621 cttacgtgat aactcaggtt gtggccttcc agtaaggtcc cacctacacc tgccagccag
    10681 ctattttgcc cgttgctgtt acggttggcg ttaaaggagg cataagcgcg gtcgagcgca
    10741 ttttcccggg tgtagcgacg tttggtcaga acattgaaag gcactgaaac attaagtccg
    10801 acaatacgtt cgttatcggg gatccctaca gattcattcc acgaaaatga gagtgaataa
    10861 ctgatgccaa cccagctgct ggtatacccc acctgatacc aggtatctga atccgaagta
    10921 ttccagtatt tttgatgggt accagagata taaagcgagc caaagtcatt aagtgattgt
    10981 gaaatattta actgcaaacg gtctttacgg ctaaagcgta aattgtggta attcacgatt
    11041 atcggttcat cccgatgctc accgtcataa tcgtaatcat attcgtaccc ctccattcga
    11101 cgatacgcaa catcatcaag ggtataaaaa ccttgtgtcg aatagcggta acccattaac
    11161 tgaaaattgg tgccgaaggt gttcatcgat ttcgcataga ggaagcgaat ggaatccccc
    11221 tcatgacgac tgtcgtcggc taactgactg cgcgcatggg tcacatccag cgacactgcg
    11281 ccccagttcc cgaggttgcg ccccagccct aataaaaagg cggtgtaatt ggcagataat
    11341 tgcgtcccgc cgtaggcagt aaattcctgt ggtaaaccgc cgagtgccgt gccctgaaaa
    11401 aagaaaggcg atgattgctg actattaccg ctacgaaaat cgcccgccgt caggtcaaat
    11461 ttgaaacgcc cttcgcgttg taaaattggc actgttgaat acggaatagt gtaattctgc
    11521 tgattgccat cgcgttcgtc gatggtgacg tccagatcgc catttgaaga tgtcgggtgc
    11581 aaatctgtaa tttcaaaagc gccgggggaa acatagcttt gatagataat aaaaccattt
    11641 tgccgaatcg ttagctgggc cgccgtacgg gcaatcccac gtaccgttgg ggcaaaccct
    11701 tgctggctat caggatacat attatcggaa gaataaagcc gtacaccacg aaatccaaca
    11761 ccatcgaaaa tatcacttcc tgtattgccg tctcccatta ccagttcact tttcagcgga
    11821 ataatggcgc gctgtaccca ggtgccaata ttattccact gttctgaatg atatccattt
    11881 ccgcgaaaat agttccagga accattgttg cgtaaacgcc acgggccaat attaatcccg
    11941 ctgagctcac taaaaaaata gctatcgtta ccattacctc tgttaccggt gaaattataa
    12001 ttcaggagta aagcaggaat accttcatcc cactcttcag gcggaatgta accgtgagcg
    12061 ctactcaaca acgctatctg aggaatagtg atgttcaggc gcatcgctgc aaaatcaaag
    12121 ttaattgtgg catcaggtac agctttcagt aaattgatgc atttattgtt ttgctgctgg
    12181 gctaactcgg gaaatgcact gctattaagg ccaattcgtt caaggagtac ctggttaaaa
    12241 cagggcatta acccacctga tttatcacct gtattttctg ttgtcgattc aaatacgata
    12301 tcctgtgaac caatgaactc atcattacgc cagagatcca cccgatacac gcccgcaggt
    12361 tgatgattac ctctctcaaa gcgcgataaa tcagcaacga atgtgccatt ctctaataaa
    12421 aatgccgggt tgaaataact ttccgcatag cttaaaaagg agggtaaaat ggcaagagat
    12481 gaataacaaa ttccggccag acaccaggta tacctctgcg gaatatccgt agtagtgggt
    12541 attttcataa ttagctctga tattgtcaga tgattcggtt agtttaagtt cacttctctg
    12601 accggggtta ctgaaccata atcattaacg gtctgaaatt tcagctttcc ctgcactcct
    12661 gagggtaagg gaatttgtga attaactctg ggtgcagcca tagcattccc caacttttgg
    12721 ctgcccactt gtaagttaac cagggtgaca taaaaaggtg atggattatg aacattgata
    12781 tagttaccgg atcgcgaaaa cttgagtgta tccggcgctt ctgcgggtaa ttcttgtagt
    12841 tgaattggac ggagaaataa cttcatgcgc gataaaatcg ccagttgcaa aacattcctg
    12901 ccgttcaatg catttttatc taccgaagga atcgtcttaa cattcatcca gaacagagac
    12961 tcacgatctg ctgccagcgg tggaccggtg taaataatac gcaaagtatt ttcgcttttg
    13021 ggttcactaa caaatagtgg cggtgtaatg atgaatgact tttcttttac accgctgctg
    13081 ttttcaatcc acgaattgac cagaaaacgc tcattggtat ggctatttct aatccatacc
    13141 gcagtctgtt tagcatcagc gggataaata atacgcgtgg cacctaatgc gatccctccg
    13201 gcagcatgga cgctggccga aatgattaaa taaaatataa tgagcatcaa taactttatt
    13261 ttagtcatca tagttaatac gccttgtgta ttcctgacgg caaaaataag aaatataaag
    13321 aaatgattgc tttggtttct tacaacacat cattcccttt tcatttaata aagcggcatg
    13381 atacaaagtg aacatgattt cgatttacat tgccaccata ccgcttcaga caaccggtac
    13441 attactttgt tataagacgt gatatataat tcaaaacaac gtggttttga ttattcgtat
    13501 ctcataataa aagtcgcgtc agcatttgct tgccctgctg atgcacttgt accggtgcct
    13561 ttataacgtg cagaaaaatg aagaacgttg gtcccgggga tcaagttctg gttggttgag
    13621 aagctattcc catcaggttt gagaattgcg gatgtattat caagtatttc aataccgaca
    13681 cccgttgctg ttgttgtatt ggtactggat gcaatggcca ataaattatc attgattgca
    13741 tcagcctgac cagaaaatgc aacggcagca ttagcggcaa caacaggatc gcagtcgttc
    13801 aactgaatgg tgaatggaat taatgcagag gtattaccaa cagcattgaa aatatcggta
    13861 cgatattgac cgagtgtgac aacctggtct gccgagtcag tattcactga acaggcagca
    13921 ttcaccagtt caccttcaaa gtgaattgta ccgccatcta caacggcagc ataactaccc
    13981 gtagcggcga atagtgcggc agccagcgca gacgaaataa atcttaattt catatatatt
    14041 ccttcaatct catttatcga ctccacatcc gtatataacc gattacttta tttaagacac
    14101 tgatagtagt aaattccttt ttatcctcta agaatgtctt aattgaaaat atgcactcta
    14161 t
//
LOCUS       DAZFDC010000122        14154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFDC010000122 DAZFDC010000000
VERSION     DAZFDC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.122"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            180..1238
                     /gene="citC"
                     /locus_tag="ACHJ48_003768"
     CDS             180..1238
                     /gene="citC"
                     /locus_tag="ACHJ48_003768"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO4709240.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIAMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTESFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1253..1549
                     /gene="citD"
                     /locus_tag="ACHJ48_003769"
     CDS             1253..1549
                     /gene="citD"
                     /locus_tag="ACHJ48_003769"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO4709241.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1546..2454
                     /gene="citE"
                     /locus_tag="ACHJ48_003770"
     CDS             1546..2454
                     /gene="citE"
                     /locus_tag="ACHJ48_003770"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO4709242.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2465..3997
                     /gene="citF"
                     /locus_tag="ACHJ48_003771"
     CDS             2465..3997
                     /gene="citF"
                     /locus_tag="ACHJ48_003771"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO4709243.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTLFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4001..4552
                     /gene="citX"
                     /locus_tag="ACHJ48_003772"
     CDS             4001..4552
                     /gene="citX"
                     /locus_tag="ACHJ48_003772"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO4709244.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPRPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4527..5405
                     /gene="citG"
                     /locus_tag="ACHJ48_003773"
     CDS             4527..5405
                     /gene="citG"
                     /locus_tag="ACHJ48_003773"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO4709245.1"
                     /translation="MSMPATLTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQLLTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5456..6919
                     /gene="citT"
                     /locus_tag="ACHJ48_003774"
     CDS             5456..6919
                     /gene="citT"
                     /locus_tag="ACHJ48_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO4709246.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7033..7839
                     /gene="rna"
                     /locus_tag="ACHJ48_003775"
     CDS             7033..7839
                     /gene="rna"
                     /locus_tag="ACHJ48_003775"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO4709247.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQVSIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8069..8479
                     /gene="rnk"
                     /locus_tag="ACHJ48_003776"
     CDS             8069..8479
                     /gene="rnk"
                     /locus_tag="ACHJ48_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO4709248.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8617..8706
                     /gene="yldA"
                     /locus_tag="ACHJ48_003777"
     CDS             8617..8706
                     /gene="yldA"
                     /locus_tag="ACHJ48_003777"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO4709249.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8710..9948)
                     /gene="ybdR"
                     /locus_tag="ACHJ48_003778"
     CDS             complement(8710..9948)
                     /gene="ybdR"
                     /locus_tag="ACHJ48_003778"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO4709250.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10169..10597
                     /gene="uspG"
                     /locus_tag="ACHJ48_003779"
     CDS             10169..10597
                     /gene="uspG"
                     /locus_tag="ACHJ48_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO4709251.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10718..12283)
                     /gene="ahpF"
                     /locus_tag="ACHJ48_003780"
     CDS             complement(10718..12283)
                     /gene="ahpF"
                     /locus_tag="ACHJ48_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO4709252.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12412..12975)
                     /gene="ahpC"
                     /locus_tag="ACHJ48_003781"
     CDS             complement(12412..12975)
                     /gene="ahpC"
                     /locus_tag="ACHJ48_003781"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO4709253.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13347..14093
                     /gene="dsbG"
                     /locus_tag="ACHJ48_003782"
     CDS             13347..14093
                     /gene="dsbG"
                     /locus_tag="ACHJ48_003782"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO4709254.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3370 a         3736 c         3552 g         3496 t
ORIGIN      
        1 tgaaataaat caaaatttca cgccgaaata ctccttagga tgtatagcga aaggagaaaa
       61 agatatacct cgatcacccc cctttctccc aagtgaaaat aaaaggttat cagtttgcaa
      121 cattgaacaa cattcgttgc aaatcgataa caacatgcac cttcaggata ctatttatta
      181 tgttcggcaa tgatattttc acccgcgtaa aacgttcaga aaataaaaaa atggcggaaa
      241 tcgcccaatt cctgcatgaa aatgatttga gcgttgacac cacagtcgaa gtatttatta
      301 ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat tgccggaaat attattaaat
      361 gcgttgctat cagtgaatcc gttcgcggtg aaggactggc gctgacatta gccactgaac
      421 tgataaacct cgcctatgag cggcacagca cgcatctgtt tatttatacc aaaaccgaat
      481 acgaggcgct gttccgccag tgcggttttt ccacgctgac cagcgtaccc ggcgtgatgg
      541 tgctgatgga aaacagcgcc acgcgactga aacgctatgc cgaatcgctg aaaaaatttc
      601 gtcatccagg gaacaagatt ggctgcattg cgatgaacgc caatcccttt acgaatggtc
      661 accgttatct gattcaacaa gctgcggcac agtgcgactg gttgcatctg tttttagtca
      721 aagaagattc ttcacgcttc ccctatgaag accggctgga tctggtgtta aaaggcaccg
      781 ccgatattcc acgcctgact gtgcatcgcg gctccgaata catcatctcc cgcgctacgt
      841 tcccttgcta cttcattaaa gaacagagcg tcattaacca ttgttacacc gaaattgatc
      901 tgaagatttt ccgtcagtac ctcgctcccg cgctgggtgt aactcaccgc tttgtcggta
      961 ctgaatcctt ttgtcgcgtt accgcccagt acaaccagga tatgcgctac tggctggaaa
     1021 cgccgactat ctccgcagcg cccatcgaac tggttgaaat tgagcggctg cgttaccagg
     1081 agatgccgat atccgcttcc cgggtacgtc aactgctggc gaaaaacgat ctcacggcta
     1141 tcgcgccgct ggtccctgca gtcacgctgc attatttgca gaacctgctt gagcactccc
     1201 gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga aacaggtgaa aaatgaaaat
     1261 aaaccagccc gccgttgcag gcacccttga gtctggggat gtgatgatac gcatcgcccc
     1321 actcgatacg caggatatcg acctgcaaat caatagcagc gttgagaaac agtttggcga
     1381 tgcaattcgc accaccattc tggacgttct cgcccgctac aacgtgcgcg gcgtacagct
     1441 gaatgtcgat gataaaggcg cactggactg cattttacgt gcacgactgg aagccctgct
     1501 ggcacgcgcc agcggtatcc cggctctgcc atgggaggat tgccaatgat ttccgcttcg
     1561 ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg tgccaatgcc
     1621 gcgatggtca gcaactcctt catctacccg gctgatgccc tgatgtttga cctcgaagac
     1681 tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg tttatcacgc gctgcaacat
     1741 ccgctgtatc gcgatattga aaccattgtg cgtgtaaacg cgctggattc cgaatggggt
     1801 gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg ttgtacgtct gccgaaaacc
     1861 gataccgctc aggatgttct ggatatcgaa aaagagatcc tgcgtataga aaaagcctgt
     1921 ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg aatctccgct gggcattacc
     1981 cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg gtatcgccct cggtgcagaa
     2041 gactatgtgc gcaacctgcg tacagaacgc tccccggaag gaactgaact gctgttcgca
     2101 cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga taccgtctat
     2161 tccgacgcta acaacgaagc cggattcctg caagaagccg cccacatcaa acagctgggc
     2221 tttgacggca aatcgctgat caacccgcgt cagattgatc tgctgcacaa cctctacgca
     2281 ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag aagccgctga agccgccgct
     2341 cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc ggttatcgat
     2401 cgtgcccgtc tggtgctctc ccgtgcagaa ctttccggca tccgcgaaga ataaggcaat
     2461 caaaatgacg cagaaaattg aacaatctca acgacaagaa cgggtagcgg cctggaatcg
     2521 tcgcgctgaa tgcgatcttg ccgctttcca gaactcacca aagcaaacct accaggctga
     2581 aaaagcgcgc gatcgcaaac tgtgcgccaa cctggaagaa gcgattcgtc gctctggttt
     2641 acaggacggc atgacggttt ccttccatca cgctttccgt ggcggtgacc tgaccgtcaa
     2701 tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac ctgatcctgg cgtccagctc
     2761 cctgagtgat tgccatgcgc cgctggtaga acacattcgc cagggcgtgg ttacccgcat
     2821 ttatacctcc ggcctgcgtg gtccactggc ggaagagatc tcccgtggtc tgctggcgga
     2881 accggtgcaa atccactctc acggcggacg tgtgcatctg gtacagagcg gcgaactgaa
     2941 tatcgacgtg gctttcctcg gcgtcccgtc ctgtgatgaa ttcggtaatg ccaacggcta
     3001 caccggtaaa gcctgctgcg gctccctcgg ctatgcaatg gttgatgccg acaacgcaaa
     3061 acaggttgtg atgcttaccg aagaactgct gccttatccg cataatccgg caagcattga
     3121 gcaagatcag gttgatttaa tcgtcaaagt tgatcgcgtt ggcgatgctg caaaaatcgg
     3181 cgctggtgct acccgtatga ccactaaccc gcgcgaactg cttattgccc gtagcgctgc
     3241 ggatgtgatt gtcaactcag gctacttcaa agaaggtttc tccatgcaaa ccggcaccgg
     3301 cggcgcatcg ctggcggtaa cccttttcct ggaagacaaa atgcgtagcc gcgatattcg
     3361 cgccgacttc gccctcggtg gtattaccgc gacgatggtt gatctgcacg aaaaaggtct
     3421 gatccgcaaa ctgctggatg tacagagctt tgacagccac gctgcgcaat cgctggcccg
     3481 taaccccaat cacatcgaaa tcagcgccaa ccagtacgct aactggggtt cgaaaggtgc
     3541 atcggttgat cgtctcgacg tggtggtact gagcgcgctg gaaattgaca cccagttcaa
     3601 cgttaacgtg ctgaccggct ctgacggcgt actgcgtggt gcttccggtg gtcactgcga
     3661 taccgcgatt gcctctgcgc tttccatcat cgtcgcgccg ctggtacgcg gtcgtattcc
     3721 gactctggtg gataacgtgc tgacctgcat caccccgggc tccagtgtcg atattctggt
     3781 cacagaccac ggtatcgcag ttaacccggc acgtccggaa ctggcagaac gtctgcagga
     3841 agcgggcatt aaagtggttt ccattgagtg gctgcgcgaa cgtgcgcgtc tgctgaccgg
     3901 tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt accgcgatgg
     3961 ctcggtgatc gatgttgtgc atcaggtgaa ggaataagcc atgcacctgc ttcctgaact
     4021 cgccagccac catgcggtgt caattcccga gctgctcgtc agccgggatg aaaggcaagc
     4081 acggcaacac gtctggctca agcgccatcc tgttccactg gtctccttta ccgtggttgc
     4141 gcccaggccg attaaagaca gtgaggtcac acgccgaatt tttaatcatg gcgtgacagc
     4201 cttgcgtgcc ttagccgcaa aacagggctg gcaaattcag gagcaggctg cactggtttc
     4261 cgccagcggg ccggagggta tgttgagcat tgccgccccg gctcgcgacc tcaagctcgc
     4321 caccattgag cttgaacata gtcatcctct cgggcggtta tgggatatcg atgtcctgac
     4381 gcccgaaggc gaaattctct cccgccgcga ctattcactg ccgcctcgcc gctgcctgtt
     4441 gtgcgagcaa agcgcagccg tctgcgcgcg tggaaaaacc catcaactga ccgatttact
     4501 caaccgcatg gaggcactgc tgaacgatgt cgatgcctgc aacgttaact aaaaccacaa
     4561 agcttgcgac gtcattaatc gatgagtacg ccctgctggg ctggcgcgcc atgctgactg
     4621 aagtcaatct gtcaccgaaa ccaggtctcg tggatcgcat taactgcggt gcgcataaag
     4681 atatggcgct ggaagatttc caccgcagcg cgctggcgat tcagggctgg ctaccccgtt
     4741 tcattgaatt tggtgcctgt agtgcggaaa tggcaccaga agcggtactc cacggattac
     4801 gcccaattgg tatggcttgc gaaggtgata tgttccgcgc caccgcgggc gtaaacacgc
     4861 ataagggcag cattttttct ttagggctac tgtgtgcggc aattggccgt ttgcttcagc
     4921 tcaaccagtt gctaacgcca acaaccgttt gttctacggc ggcaagtttc tgccgtggcc
     4981 tgaccgatcg cgaactgcgt accaataatt cacaactgac ggcaggtcaa cggttgtacc
     5041 agcagcttgg ccttaccggc gcacgcggtg aagccgaagc gggttatcca ctggtgatca
     5101 accacgcctt gccgcattac ctcactctgc tggatcaggg gttagatcct gaactggcat
     5161 tgctcgatac cttgctccta ctgatggcga tcaacggcga taccaacgtt gcttcgcgcg
     5221 gtggcgaggg gggcctgcgc tggctacagc gcgaggcgca aacattattg caaaaagggg
     5281 gcattcgaac ccccgcagat ctcgattatc tccggcagtt cgacagggag tgtatcgaac
     5341 gaaatctcag tccaggcggc agtgccgacc tgctgatcct tacctggttt ttagcacaga
     5401 tttaattatt taagaacttg ataaatttgg aaatattaat tttcggagaa cccgtatgtc
     5461 tttagcaaaa gataatatat ggaaactatt ggccccactg gtggtgatgg gtgtcatgtt
     5521 tcttatccct gtccccgacg gtatgccgcc gcaggcatgg cattacttcg ctgtgtttgt
     5581 ggcaatgatt gtcggcatga tcctcgagcc aattccggca acagcgatca gttttattgc
     5641 ggttactatt tgcgttattg gcagtaatta cctgctcttt gatgccaaag aattagctga
     5701 cccagcgttt aatgcgcaaa aacaggcgct gaaatggggc ctggctggtt tttccagcac
     5761 tacggtatgg ctggtatttg gcgcatttat ttttgcatta gggtatgaag tttccgggtt
     5821 aggtcgtcgc attgcccttt tcctggtgaa attcatgggc aaacgcacgc tgacgttggg
     5881 ttatgcgatt gtcattatcg acattctgct ggcaccgttt acaccgtcca acaccgcgcg
     5941 taccgggggt acggtttttc cggtcattaa aaacctgccg ccgttgttta aatcattccc
     6001 gaacgatccg tccgcgcgtc gtattggcgg ctatttgatg tggatgatgg tcattagtac
     6061 cagtctgagt tcgtccatgt ttgtcaccgg tgcggcacca aacgtgctgg gtctggagtt
     6121 cgtcagcaaa attgccggta tccagattag ctggttgcag tggttcctct gcttcctgcc
     6181 ggttggggtt atcttgctta tcattgcgcc gtggctttcc tacgtgctgt acaaaccgga
     6241 aatcacacac agtgaagaag tggcaacctg ggcgggtgat gaactaaaaa ccatgggtgc
     6301 gctgacacgc agagagtgga cgctgattgg ccttgtattg ctcagcttag gtttgtgggt
     6361 atttggcagt gaagtcatta atgctactgc ggttggtctg ctggcagttt cgctaatgct
     6421 ggctctgcac gttgtgccgt ggaaagacat tacccgctat aacagcgcat ggaacacgct
     6481 ggtcaacctg gcaactctgg ttgtgatggc taacggcctg actcgttctg gttttattga
     6541 ctggttcgcc ggtaccatga gtacgcacct ggaaggattc tcaccaaacg caacggtgat
     6601 tgtactggtt ctggtgttct actttgcaca ctacctgttt gccagcctgt ctgcgcacac
     6661 cgcaaccatg ctgccggtta ttctggccgt cggtaaaggt attccgggcg taccaatgga
     6721 acaactgtgt atcctgctgg tgctgtctat cggtatcatg ggctgtctga cgccgtatgc
     6781 aaccggtcct ggggtgatta tttacggctg tggctatgtg aaatcaaaag attactggcg
     6841 tcttggcgca atcttcgggg tgatttacat ctctatgttg ctgttggttg gctggccgat
     6901 tctcgccatg tggaactaat acgttcgcca aacgctgaat aaatcatcac gcccgccagg
     6961 ttagcccacc tggcgggctt ttttatgatt taatagatag tattcagtgt atgtatgagt
     7021 tccacaccca ttatgaaagc attctggcgt aacgccgcgt tgctcgcggt ttctctgctt
     7081 cccttctctt ctgccaacgc cgtagcgttg caggcaaaac agtatggcga ttttgatcgc
     7141 tatgtcctgg ccctctcctg gcaaaccgga ttttgccaga gtcaacacga tcgaaatcgt
     7201 aacgaacgag atgaatgtcg cctgcaaacc gaaacgacca acaaagctga ttttctgacc
     7261 gtacatggtc tgtggccagg attgcctaaa tcggttgctg cccgtggtgt tgatgaacgc
     7321 cgctggatgc gcttcggttg cgctactcgc ccaatcccga atctaccaga agcgcgcgcc
     7381 agccggatgt gttcatcgcc ggaaaccgga ttatcactgg aaacggccgc taaactaagt
     7441 gaagtcatgc caggagctgg cggacgttcc tgcctggaac gctacgaata tgccaaacac
     7501 ggtgcctgct ttggttttga tccggacgca tacttcggta cgatggtacg cctgaatcaa
     7561 gagataaaag agagcgaagc cggaaaattc cttgcggata attacggtaa aacagtgagc
     7621 cgccgtgact ttgacgccgc ctttgccaaa agctggggaa aagagaacgt gaaagcagtt
     7681 aagctaacgt gccagggtaa ccctgcgtat ttgactgaaa ttcaggtctc gatcaaagct
     7741 gacgccatca acgctccgct ttctgcaaac tcattcttgc cacaaccaca cccaggtaac
     7801 tgtggcaaaa cctttgtgat tgataaagcg ggttattaat cctctgattt acaaaacacg
     7861 ctaattcgtc tttcataaac catgacgatt aagaattata cataatgcgc tgcattgagg
     7921 aattccctga atgtgacgca aatcacttca aagagtgagc aacagtcagt atcatcatgt
     7981 gagttaaacc ctcgccgcct gacggtgagg gttttctttt gggtatgttt atctgcgaca
     8041 ctcgcagtac cgacgacatg gagtaaaaat gtccagacca actatcatca ttaacgacct
     8101 ggatgccgaa cgcatcgata ttctgctgga gcaacccgcc tatgctggtt tgccaatcgc
     8161 cgacgcgtta aacgcagagt tggatcgcgc ccaaatgtgt tcgccagaag agatgccaca
     8221 cgacgtggtg acaatgaaca gccgggttaa attccgcaat cttagcgatg gcgaagtgcg
     8281 tgtgcgcacg ctggtgtatc cggcaaaaat gaccgatagc aatactcagc tttccgttat
     8341 ggctccggta ggtgccgcac tgctgggact gcgcgttggc gattccattc actgggaact
     8401 tccgggcggc gttgcaaccc accttgaagt gctggaactc gaataccagc cagaagctgc
     8461 tggcgactac ctgctttaat cccatctgta caagcgataa ctcgctttgc ccagaggatg
     8521 cactcgcatc ctctttatgc aaaatcagca atttttcctg gttctcactg ttcacttttg
     8581 ctgcaaaatt attccgtttc gtttgtggga ataaccatgg cagaagcatt ttacatcctg
     8641 ataggatttc tgattatggc ggcgattatc gtcatggctg tcctttacct cgaaaaccac
     8701 tcctgacgct catattgttc cccccggcat cgcattcacc agacctgaaa ccgccttctg
     8761 cgccgcctct gcgctttgtg caccgggtac cagaatcacc ttacggcact cctcttcacg
     8821 cttttcaaag atctcatatc cccgggcggc ctcttcaaac ggcatatagt gggtaacaat
     8881 ttcttctggt ttcagtaatc ctttctcaat taacggtaat aattctccca gccatgcgtg
     8941 aacgtgggtc tgtcccattt taaacgtcaa ccctttatca aaggcgtcgc caaacaggaa
     9001 accgtgaata aatccagcgt agacgcccgg tacgctaaca atgccgccac gcctgaccgc
     9061 cgcaatacac tgacgcaacg ctttaccgct gctgccctcc agtttcaggt tagtcagcac
     9121 cgtttccgtg gtgctgcctt tcgcttcaaa accgacggcg tctattactg catccacgcc
     9181 ccggtgacct gccgtttgtt caataattga ctgtgccgga tcgctatctt catcgaaatt
     9241 aatcgggatc gcgccgtagc ggtcagcggc gaaacgcaag cggtagggat gatgatcaac
     9301 aacaaaaatc tgttccgcac cgagcaaccg tgcacaggcg attgtcaaca atcccacagg
     9361 accggcacca tagacagcaa cgcttgaacc ttgttggatc tgcgcatttt ttgctgcctg
     9421 ccatgccgtt ggcagaatat cagaaaggaa aagggcttta tcatctgaaa gcaaaggcgg
     9481 tactttaaac ggccccacat tccctttagg gacacggaca tactccgcct gtccaccagg
     9541 aacgccacca tacaggtgac tataaccaaa caatgccgcg ggagctggta tctgtttttt
     9601 attgagcgca gcgcctttac ccgcattggt attttcgcag gcggcatatt gctgcagtcg
     9661 acagaaaaaa cagtcgccac aagcaatgac gaacggaatt accacccggt cgcctttttg
     9721 caaatttttt acatcctttc cggtttcaac tacttccccc ataaattcat gaccaaaaat
     9781 atcgccatgt ttaacctggg gtattttgcc tcgataaaga tggaggtcag agccacagat
     9841 cgccgttgcc gttatacgca gaataatatc atctgcctgt tcaatgcccg gatcgggaac
     9901 attttctacc tgaacgtgat gtgggccgtg ataagtcaat gctttcatgc aacctccaga
     9961 gacagttcaa aaaagagtca gtctttaagg gtagctatca cagcgggaaa acgggcggta
    10021 ccgcaggttt tgggagaatt cctggtgttc agtgagaata tattcatcac agaaacagtt
    10081 cgtaaggaaa aagaaccaga caaatatcag ggataaatat tataatcatg gcgaatgatt
    10141 tgttcatgat taacagggag aaagggttat gtataagaca atcattatgc cagttgatgt
    10201 atttgaaatg gaattgagcg acaaagctgt tcgccacgct gaattcctcg cccaggatga
    10261 cggagttatt catctacttc acgtactacc cgggtcagcc agcctgagcc tgcaccgttt
    10321 tgccgctgat gtgcgtcgtt ttgaagagca tctgcaacat gaagcagaag aacgtttgca
    10381 aacgatggtc agccacttca ccatcgatcc ttcccgcatt aaacaacatg tccgttttgg
    10441 tagcgtgcgg gatgaagtca atgagttagc agaagaactg ggggctgatg ttgtagttat
    10501 tggttctcgc aacccatcga tttcgaccca tctgttaggt tctaacgcct cgagcgtgat
    10561 ccgccacgcc aatctgcctg tactggtcgt tcgctaacag ataaaagtat ttatgccgcc
    10621 ctgcctggaa catcccggca gggcttttta atgccataaa aaagccgcca ggtttgaccc
    10681 cggcggctaa gcaattgcag gtgaatctta cttcttctta tgcagttttg gtgcgaatca
    10741 ggtagtcaaa agcactcaga gaggctttgg caccttcgcc agtggcgatg atgatctgct
    10801 tgtacggaac cgtcgtacag tcacccgctg cgaacacgcc tttcacgttg gtttcgcatt
    10861 tcgcatcaat gataatctcg cccatgcggt tacgttcgac tgcgccttcg agccagttgg
    10921 tgttcggcag cagaccaatc tgcacgaaaa taccggccag ttcgatgttg tgaatatcgc
    10981 cgctgacacg atctcgatat tccagaccaa cgactttgct gccgtcgcct ttcacttccg
    11041 tggtttgcgc attcagaata atgtcgacgt ttttcaggct gcgcagtttg tcctgcagaa
    11101 cctggtcggc tttcatttct ggcgcaaatt ccagcagcgt tacgtgctca acgatacccg
    11161 ccaggtcaat tgccgcttcc acgccggagt taccgccgcc gataaccgct acgcgtttac
    11221 ctttaaacag cgggccgtcg cagtgcgggc agtaggtcac gcctttggtg cgatactgat
    11281 cttcgcccgg aacgttcatg ttgcgccatt ttgcaccggt cgccacgata atgctgcgtg
    11341 ctttcagtac cgcgccagaa gctgtttcaa tctgatgcag accaccttca actgctgctg
    11401 ggatcagttt gctggcgctc tggctgtcga tcacatcaac gtcgtattca tcaacgtgaa
    11461 ctttcagtgc gcctgccagc ttctgccctt cagtcttcgg tacagaaatg tagttttcga
    11521 tatcaacggt atcgaggatc tgaccaccaa aacgttcgcc catcagaccg gtacggatgc
    11581 ctttacgtgc ggagtaaatt gctgccgctg cacccgccgg gccggaaccg acgattaata
    11641 cgtcataagc atcacgcttg ttcagctctt ctgccgcacg tttttccgcg cccgtatcaa
    11701 ttttggcaac gatttcagtc aaagtcatgc ggccctgacc aaactctttc ccgttcacga
    11761 acactgccgg aacgcccatc acgttacgat cagtgatctc gttctggaag gtgccgccgt
    11821 caattgcagt gtgcttgatg cgcgggttca atacgctcat caggttcagc gcctgtacca
    11881 cgtccgggca gttatggcaa gagagcgagt aataggtttc gaattcaaaa tcaccgtcaa
    11941 tatggcgaat ctgctccagc agagactgcg cttctttcga cggatgacca ccggtccaca
    12001 gtaacgccag taccagcgag gtgaactcgt ggcccagcgg ggagcctgca aaacgtggcc
    12061 cctggttaga acctgggttg gtgatcagga aagacggctt acgcaccggc aagctgttat
    12121 cttctttaaa ggtgactttg tctgacagtt ctgcgatttc agccaacagt tccttgattt
    12181 ctgccgattt agcgctgtca tccagcgtgg caattaactc aacaggcttg gtcaatttct
    12241 caaggtaagc cttgagttga gttttcatat ttgtgtcgag catgtttatc tcctgggctt
    12301 taaacatcat catgcaagcg gcctctgcgg ctacctggat gcagcttgca tcatggtgcg
    12361 gggaagaaaa tcgggtgccg cagcacccga ttaattacgc gattgcggaa cttagatttt
    12421 accaaccagg tccagagacg gagccagagt tgcttcacct tctttccatt tagccgggca
    12481 aacttcacct gggtgagaag ctacgtactg tgctgctttg attttacgca gcaggtcaga
    12541 cgcgtcacgg ccaatgcctt cagcggtaac ttcgattgcc tggatgatac cctgcgggtc
    12601 aacaacgaag gtcgcacggt cagccagacc ttcatcttca cgcatgttgt cgaagttacg
    12661 ggtcagggcg ccagtcgggt cgccgatcat cgcatatttg attttagcga tggtttcaga
    12721 gctgctgtgc catgctttgt gggtgaagtg agtatcggta gatactgcgt atacgtctac
    12781 gcccagtttc tgcagttctt cgtagtggtc agcaacgtca cccagttcgg tcgggcatac
    12841 gaaagtaaag tcagccgggt agaagaagaa gacgctccag cggccttcgg tatctttttc
    12901 ggtgatttcg atgaattcgc cgtttttgaa tgcctggttt ttaaaaggtt taattttggt
    12961 gttaatcaag gacatctata cttcctccgt gttttcgatg agatgtaagg taaccaattt
    13021 ttaccgattc ggctaatgcg tttccattat caaatcgata agttttacct tacaaccttc
    13081 gtaagacaac tttgtgaact ttgcaccgcc agcggcgata aaaagtaaaa tggccccctt
    13141 tccaggtgac cgtgtaatct gaactaccct ttggtatctt cagcgccagt ctgcaggctg
    13201 cgttgttgct cttacaaatg caacaacgac atggattaca acaccctcat aaacaaaggg
    13261 caatcacctg atctaagctc ttacctatga caatgatagg ttatgccttt tactcgactt
    13321 ttgcactgac tgaaaaggac aaattaatgt taaaaaagat acttttactg gctctgcttc
    13381 ctgcaatcgc cttcgcagag gaacttcctg ctccagtaaa agcgattgaa aaacagggca
    13441 ttacaatcat caaaacgttc gatgcccccg gaggaatgaa aggttatctc ggaaagtatc
    13501 aggatatggg cgtcaccatc tacctgactc cagatggtaa gcacgctatc tctggttaca
    13561 tgtacaacga gaaaggtgaa aacctgagta acacacttat cgaaaaagaa atttacgcac
    13621 cagccggacg cgaaatgtgg caacggatgg aacaatccca ctggctcctc gacggtaaaa
    13681 aagatgcgcc ggtcattgtc tacgtcttcg ccgatccgtt ctgcccatat tgtaaacagt
    13741 tctggcagca ggcgcgcccg tgggtagatt ctggcaaagt acagttgaga acattgttgg
    13801 ttggggttat caaaccagaa agtccggcga cagccgcggc aattcttgcc tccaaagatc
    13861 ccgcaaaaac ctggcaagaa tatgaagcct ctggtggcaa gcttaagcta aacgtgcctg
    13921 caaacgtaag tacagagcaa atgaaagtgt taagcgacaa cgagaaactc atggacgatc
    13981 tgggggcaaa tgtcactccg gctatctatt acatgagtaa ggaaaatacg ctgcaacagg
    14041 ccgtggggtt gcccgatcag aaaacgctta atatcattat gggaaataaa taaaaggtaa
    14101 ttcatggatg gttgaactat atccctgctt ttcaaccatc ctaaaaaagc atga
//
LOCUS       DAZFDC010000123        13956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFDC010000123 DAZFDC010000000
VERSION     DAZFDC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.123"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            137..646
                     /locus_tag="ACHJ48_003783"
     CDS             137..646
                     /locus_tag="ACHJ48_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO4709255.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIESTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPYAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            643..2061
                     /gene="phrB"
                     /locus_tag="ACHJ48_003784"
     CDS             643..2061
                     /gene="phrB"
                     /locus_tag="ACHJ48_003784"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO4709256.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARLLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRNVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHJ48_003785"
     CDS             complement(2211..3692)
                     /gene="dtpD"
                     /locus_tag="ACHJ48_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO4709257.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELRVIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHJ48_003786"
     CDS             3963..4706
                     /gene="ybgI"
                     /locus_tag="ACHJ48_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO4709258.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHJ48_003787"
     CDS             4729..5385
                     /gene="pxpB"
                     /locus_tag="ACHJ48_003787"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO4709259.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHJ48_003788"
     CDS             5379..6311
                     /gene="pxpC"
                     /locus_tag="ACHJ48_003788"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO4709260.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHJ48_003789"
     CDS             6301..7035
                     /gene="pxpA"
                     /locus_tag="ACHJ48_003789"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO4709261.1"
                     /translation="MKIDLNADLGEGCTSDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRKNFGRSAMQLPPETVYAQTLYQIGALATITRAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQSGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSAFAEKGIVVAA"
     gene            7071..7862
                     /gene="nei"
                     /locus_tag="ACHJ48_003790"
     CDS             7071..7862
                     /gene="nei"
                     /locus_tag="ACHJ48_003790"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO4709262.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7859..8905)
                     /locus_tag="ACHJ48_003791"
     CDS             complement(7859..8905)
                     /locus_tag="ACHJ48_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO4709263.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLEVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9054..10115)
                     /locus_tag="ACHJ48_003792"
     CDS             complement(9054..10115)
                     /locus_tag="ACHJ48_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4709264.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10112..10843)
                     /locus_tag="ACHJ48_003793"
     CDS             complement(10112..10843)
                     /locus_tag="ACHJ48_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4709265.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKTPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(10858..13317)
                     /locus_tag="ACHJ48_003794"
     CDS             complement(10858..13317)
                     /locus_tag="ACHJ48_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4709266.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALTAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWMGALSFDVTWADSHFDTQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITLLNLNLYANILHQSWWN
                     ADTSTTANITVGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13367..13933)
                     /locus_tag="ACHJ48_003795"
     CDS             complement(13367..13933)
                     /locus_tag="ACHJ48_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4709267.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVTLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
BASE COUNT         3381 a         3458 c         3798 g         3319 t
ORIGIN      
        1 attcgctgct ttgatgatga tgtaaaatga ttgtttttga actggatata acagcttggt
       61 agggaagaaa gcataacccc acaaaattca tttaccatcg tctacactta ctcctgtaaa
      121 ccgttcagga gcagtaatga atcagcaacg atttgatgac agcactctaa tccgtatctt
      181 cgcgcttcat gagttacatc gactgaaaga acatggctta acgcgcgggg cgcttctcga
      241 ttatcacagc cgctataaac tcgtatttct ggctcattcc cagccggaat accgcaaact
      301 tggcccgttc gtggctgata ttcaccagtg gcaaaatctg gatgattttt acaaccagta
      361 ccgccaacgc gtaattgttt tgctttctca tcccgccaac ccgcgcgatc acaccaacgt
      421 tttgatgcac gttcagggtt attttcgccc gcatattgag tccacagaac gccagcagct
      481 ggctgcgctt atcgacagtt atcgccgtgg cgagcaaccg cttcttgcgc cgctgatgcg
      541 tatcaaacac tatatggcgc tttatcctta cgcctggctt tcagggcagc gttatttcga
      601 actttggccg cgtgtgatta acttgcgcca ttcaggagtt ttatgactac ccatctggtc
      661 tggtttcgcc aggatttacg tctgcatgat aatctcgcgc tggctgccgc ctgccgcaat
      721 tcatctgcac gcctgctggc gttgtatatc gctacaccac gccagtgggc ggcgcataat
      781 atgtcgccgc gtcaggctga acttatcaat gctcaactga acgggctaca aatcgcgctg
      841 gcggaaaaag gcattccttt attgttccgt gaagtggatg actttgccgc cagcgtcgaa
      901 atagttaaac aggtgtgcgc ggaaaacagc gttactcatc ttttttataa ctatcagtat
      961 gaggtgaatg agcgggcgcg ggatgtgcag gttgagagaa ctctgcgtaa cgtggtgtgt
     1021 gaaggatttg atgacagcgt gatccttccg cctggcgcgg tgatgaccgg caatcatgag
     1081 atgtacaaag tctttacgcc ttttaagaat gcctggctga aacggctgcg ggaagggatg
     1141 ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat cgataaagcc cgcgccatcc
     1201 attacgctga attatcctcg tcagtctttc gatactgcgc atttcccggt ggaagaaaaa
     1261 gcggcgattg cgcaattacg ccagttttgc cagaacggtg ccggagaata tgagcaacaa
     1321 cgagattttc cggcagtgga aggcaccagt cgtttgtccg ccagcctggc aacgggcggg
     1381 ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac agccgcaggc gctggacggt
     1441 ggggccggta gtgtctggct taatgagctg atctggcgcg agttctaccg tcatctgatg
     1501 acgtattacc cctcgttgtg taaacattgt ccgtttattg cctggacgga tcgtgtgcag
     1561 tggcagagca atcccgcaca tttacaggcc tggcaggaag gcaaaacggg atacccgatt
     1621 gtcgatgctg ccatgcgtca gcttaacagc actggctgga tgcataacag gctacggatg
     1681 attacagcca gttttctggt gaaagatttg ttgatcgact ggcgcgaagg cgagcgatat
     1741 ttcatgtcgc agctgattga tggtgatttg gcagccaata acggtggctg gcagtgggcg
     1801 gcttctacgg gtactgatgc agcgccgtat tttcgtattt tcaacccgac aacccagggc
     1861 gagaaatttg accgcgaagg cgagtttatc cgccagtggc taccggaact gcgcaatgta
     1921 ccggggaaat cggtgcatga gccgtggaag tgggctgaga aagcaggtgt gaagctggat
     1981 tatccgcaac cgatagtcga gcacaaagag gcgagagtac aaacgttggc agcgtatgag
     2041 gcggcgcgga aggggaaata acagacgcgt caggcaatcg agcccagatg ccggatgcgg
     2101 cgtgaacgcc ttatccagcc tacaaatttg tgcaaattca ataaattgca ggaaatacgt
     2161 aggcctgata agacgcgtca agcgtcgcat caggcatcgg tgctggccta ttaagactcc
     2221 agcgccagcg cgcggtttct gaatttcagc gcctgataca gccaaatcat cagtaccact
     2281 cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata cttcaatata tgcattgatg
     2341 gagtagttga tcgccccgga agcatcaaac gaagcctgcg atgtctgatc ggcaatcacg
     2401 cccgccagat agttcgcaat cgcgccggaa agcagcatat aaatgccggt taatacgccg
     2461 gtcacaccgg ggatttcaat gcgcgtaatc tgcgacatag caaccgggtc gataaacagt
     2521 tccgcaaagc ccatcaccgc caggcctaat accatcagtg gcagagaaga gtgaccatac
     2581 attgcggacc agcgggcgct taaggtcaga atgcagaatc cggcgctcat caggccaagg
     2641 ccaagagcaa attttcccca gatgcgcacg gtacgattac ccgcgacact ctcttttacc
     2701 acccacgcca ggaacacacc gcacagcata actgcgaagg cattaatcga ctggaacatc
     2761 gcggtcggaa cggtataacc aaacatatcg cgattaacga agcggtcgat ataaaggcta
     2821 atcgagctac cgccctgttg tgcgaaggcc cagaacaaca tactgaagaa ggtgagcgtc
     2881 acaatcaccc tcagctcctt ccgctgtttc tggttttctg ctttgcgata aatttttgcc
     2941 agtacaccca ggccaatgat agtcgcgaca atcaaggcgt ataccgacca ttctttccag
     3001 aacagtacgg taatcagcgc aggcgttgcc accagcagaa ccagcagcca tccccagttc
     3061 ggcagcagaa agtttgtcgc acgcagtact tttttgttaa cgccgcgggt atgtgtgaaa
     3121 tgacgattgc cacataagaa aataaccaga cccgcgatca tacccaccgc cgccaggcca
     3181 aagcccatcg cccaactgta ctcttcctgg gcgtagccac aggcaatagg tgcgataata
     3241 gaccccacgt tacccgctgc atacatcagc gagaaaccgc catcacgacg cggatcggtt
     3301 ggctcataca gctcgccgag caggcaactg acattggatt taaacagacc atagccacaa
     3361 acgattatcg ccagggacag atagaggaat gacggatgga tctcacttgc acccaacacc
     3421 acatgaccga tcgccatcaa taacgccccc agcatcaccg ccatgcgatt gccgagaact
     3481 ttatccgcca aaaagccacc gaggattggc gtgacataca ccagcgaaca gtaggcgcta
     3541 aataactcgt aggcgtgagt atcgttgtat tttagttgat tggtgagata gagaatcagc
     3601 agggcacgca tgccgtaaaa gctaaaatat tcccagattt gcagcgcaac gacatagtaa
     3661 atagcgcgcg gttgtgatgc gtgtttattc ataataattc cacaattgac ggcaacgtca
     3721 cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta aaactttgat acagatctga
     3781 ttatttatgc aatatgctgt ctgattgcat aaatatacat tagctgaagc gtgatgatat
     3841 aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat atgtcaaaag attggctatc
     3901 gacgaacagg cgaggtaacg ctatgttaac ggtgtagcta tcagaatacg gaaggcgcga
     3961 agatgaaaaa caccgaactg gaacaactga ttaacgaaaa actgaacagc gcggcgatta
     4021 gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga gacggtgcaa aaaattgtta
     4081 ccggtgtcac tgccagccag gctttgctcg atgaggcagt gcgtctgggc gcggatgcgg
     4141 tcattgtgca tcatggctac ttctggaaag gcgaatctcc ggtcattcgc ggcatgaagc
     4201 gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct gtatggctgg catttgccgc
     4261 ttgacgcaca tcctgagctg ggcaataacg cacagctggc ggcgttactg gggatcacgg
     4321 tcatgggcga aattgagccg ttggtgccgt ggggcgaact gaccatgcct gtgccgggac
     4381 tggagctggc ttcctggatt gaagcgcgtc tgggacgtaa gccgttatgg tgtggcgata
     4441 ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg cggcgggcaa agttttatcg
     4501 atagcgccgc gcgttttggc gtggatgctt ttattactgg cgaagtttct gaacagacca
     4561 ttcattcagc ccgcgagcag ggattgcatt tttatgctgc aggtcaccat gccactgaac
     4621 gtggtggtat tcgcgcattg agcgagtggc tgaatgaaaa taccgatctt gatgtgacct
     4681 ttattgatat tcctaatcct gcataacgaa taatcagagg gatcgaaagt gcaacgagcg
     4741 cgttgttatc tgataggtga aacggcggta gtgctggaac tggaaccgcc ggtgacgctg
     4801 gctagccaga aacggatctg gcgactggcg cagcgtctgg tggatatgcc gaatgtggtt
     4861 gaagccattc ccggcatgaa caatatcacg gtgattttgc gtaatcctga gtcgctggcg
     4921 ctggatgcca tagagcgttt gcaacgctgg tgggaggaga gcgaggccct ggaacctgag
     4981 tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag gcggaccgga tttggcggtg
     5041 gtcgcggcgc attgcgggtt gagcgaaaaa caggttgttg aattgcactc ctccgtggaa
     5101 tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt atctcgggag tttgccggaa
     5161 caactacaca cgccacggcg cgctgaaccg cgcttgctcg ttccggcagg ttctgtcggg
     5221 atcggcgggc cgcagactgg tgtttatccg ctggcaacgc cgggtggctg gcagttgatt
     5281 ggtcatacct cactcagcct gtttgatccg gcgcgtgacg aacccatctt attacgtccg
     5341 ggagacagcg tgcgctttgt accgcaaaag gagggagtat gctgaagatt attcgtgcgg
     5401 gcatgtatac cactgtgcaa gatggcggtc gtcacggttt tcgccagtcg ggtatcagcc
     5461 actgcggcgc actggatatg cctgcgttac gcattgctaa cctgctggtg ggtaatgatg
     5521 ccaatgcccc cgcgctggag atcacgctcg gtcagttaac ggttgagttc gaaactgatg
     5581 ggtggtttgc tctgacgggt gccggttgcg aagcgcggct ggatgataat gccgtctgga
     5641 ccggctggcg attgccgatg aaagcaggcc agcgtttaac gcttaaacgc ccgcagcacg
     5701 ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt tccgccggta atggggtcat
     5761 gcagcaccga tctcaaagtg gggattggcg ggctggaagg ccgtttactg aaggatggtg
     5821 accgactccc gattggcaaa gcgaagcgtg attttatgga agcgcagggc gttaaacagc
     5881 tgctgtgggg caaccgcatt cgcgccttgc cggggccgga atatcatgag ttcgatcgcg
     5941 cctcgcagga tgcattctgg cgttcgccct ggcagcttag ctcgcaaagt aaccgcatgg
     6001 gctatcgctt acaggggcaa attttaaaac gcaccaccga tcgcgaactg ttatctcacg
     6061 gtttgttacc gggcgtggtg caggtgccgc ataacgggca gcccattgtg ttgatgaacg
     6121 acgcacagac caccggtggt tacccgcgta ttgcctgtat cattgaggct gatatgtacc
     6181 atctggcgca aattccgctc ggtcagccga ttcattttgt ccagtgttca ctggaagagg
     6241 cactgaaagc gcggcaagat cagcaacgtt atttcgaaca attagcgtgg cggctgcaca
     6301 atgaaaattg acctgaacgc cgatctgggc gaaggctgca ccagcgacgc agagctatta
     6361 acgctggttt cctctgccaa tattgcctgt ggatttcatg caggcgatgc gcaaaccatg
     6421 caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga ttggcgctca tccgagtttt
     6481 cccgacagga aaaattttgg tcgcagcgct atgcagctgc cgccagaaac cgtttacgcc
     6541 cagacgctgt atcaaattgg cgcgctggca acgattaccc gtgcgcaagg tggtgtaatg
     6601 cgtcatgtca aaccgcacgg catgttatac aaccaggcgg cgaaagaggc acaactggca
     6661 gacgccatcg ccagagcggt atacgcttgc gatccagcat tggttctcgt tgggctggca
     6721 ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga caacgcgcga ggaagtgttt
     6781 gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc gaagccagtc aggcgcgctg
     6841 attgaagacg aagaacaggc gcttgcgcaa acgttggaga tggtgcaaca cggcagagtc
     6901 aaaagtatca ccggcgaatg ggcaacggtc acggcgcaaa cggtctgcct gcatggcgac
     6961 ggcgagcacg cactggcgtt cgcccgccga ctacgctctg catttgccga aaaggggatt
     7021 gttgtcgcag cataaccccg attaataaag aatgaaaaaa ggatatcacc atgcctgaag
     7081 gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc aaaccactaa
     7141 ctgatgtctg gtttgccttc ccgcagttaa aaacttatca atcacaactt atcggtcaac
     7201 acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc aacgacttaa
     7261 cgctctacag ccataatcag ctttatggcg tctggcgcgt ggttgatacc ggcgaagagt
     7321 cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc attctgcttt
     7381 atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat ccgtttttac
     7441 aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg aaagaacggt
     7501 tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat caggcgtttc
     7561 tggctgggct tggcaattat ttgcgtgtgg agatcctctg gcaggttggg ttgactggaa
     7621 atcataaagc gaaagacctc aatgcggcgc aactggatgc actcgcacac gcgttactgg
     7681 atattcctcg gttttcttac gctacgcggg ggcaggtgga tgagaataag catcatgggg
     7741 cgctgtttcg ctttaaggtt tttcatcgtg atggcgaact gtgcgaacgc tgtggcggca
     7801 tcattgagaa aaccacgctg tcatctcgcc cgttttactg gtgtccgggc tgccagcact
     7861 aggccgtacg tttcggcgca taggttgaaa taaaccgcgc aatggcaggc cctgtcagca
     7921 aaatactgaa caggcgcagg gtttgcatcg ccataatgag cgccatatcg gcattgctcc
     7981 ctgcggcgat gaccgccacg gtatcaagcc cgccggggct ggtggcgagg taggcagtca
     8041 taaaatcaat gtgcataaac cgggtcagtc cccacgccat acccgcacaa atagccagca
     8101 gagcaaaaat cgacagcagg atttgtggta gcgggcgtag tgcccgcagt aagatttgtt
     8161 tatcgaaacc aaggccaatc cgccagccaa ttgccatata cgccatcgcc agtagccatt
     8221 ccggtagttc gatggtgatg agctgaccag actggagcac tgcgcccgcc agcatcggga
     8281 tgagcatcgt gccggaagga agacgcagca ggcatcccac tgtaccggca acgaccgcga
     8341 gcaaaatagt aagcaggaga ttaatgctca ctggcgggaa ccagacgata tgctggttaa
     8401 ccgcttcagc gttatcgccc agcatcattc gcgtgaccag aactgcggcc cccgcgacaa
     8461 acagcactcg cagatactgc ataaacgcca ccaggcggat atctgcgccg taatcctgcg
     8521 ccatcgcgac cattgccgcc gccccgccag gcgaggagcc ccaggcacca gtatttccgg
     8581 gcagtgagct atagcgcacc agtagccatc ccacgatagc gctggaaagc aacgtcacca
     8641 gtaaaatagc taacacaatc ggccagttaa cctccagcgt ggtcaggatc gaaccggtca
     8701 agttttgcgc aatcatgcag ccaagaatgg cctgcgcggc gagaaaagcg gagcggggga
     8761 gttgcagggt aaccccgcgc atactgaaga tgatcccggc gatcatcggt ccaagtaaga
     8821 gtgccgccgg gagatgtaat ccgaggaaac caatagaaag gagaagtgat agcacacata
     8881 acattcccca ctgcaaaact ggcatccttt gctccctcat tattttctct ttggtgtatc
     8941 gatacagcat aaagaatgcc gggcatggaa aaaataacgg taatcaataa aagccggaaa
     9001 attggcaatt aaccgaagaa gccctctccg gctggaaagg gcaataggct aaatcagttg
     9061 tagttaattt tgaaaagtac cactgtatca aagggaccaa cgtcgatggg cgtacccggc
     9121 gtggatgaca attctgccgt gtaggttttt tcatacacta acaggtcgct ggtaaaatcg
     9181 gcatactcac tgtatttgtt gaaagtatag ggtgaggcat tttcatccaa caatcgaagc
     9241 ttcaagccat tgccaatgag cagtgattta tcttcgtcga taagggtttc atcggtataa
     9301 aacgaagagc tgactttaaa accgtcggta cactggtcgt tttgtgattt ggttgtagtt
     9361 attttaaatt gctgcgacat cgtatggcga cgaatatcca gcacgttaaa tcggccaaaa
     9421 tcaacgacct gggtttcagg tgagatagca aagttgacgc tgcaatccag cacgcggata
     9481 ttttccagac cggtaatgtg atatttcaga ttcttcgcgg tagggtcttc gttcacgctg
     9541 cctgcgccat caaactgtac gacgatgtaa tcgctgagtt tgctctgata atcatggggc
     9601 ggcatggcgc gaactttaac gtaaagccgc atacgcgcca caaacgtccg cgatttgtgc
     9661 ataacggttg gatcgccgga gcaaagttta ttttgccagc ccatcgcaat gatctgctcc
     9721 ggggtgtaaa tatcaatatt cttactgtcg atgcactgat tcgtgtcgat gcggcttttc
     9781 ccaaggcttg cgtcatagtc cacgccttca taggttacgc ccaattgata atagggatcc
     9841 tgaataccgg ggtaagggtt tacccaggcg tagacgtgct cgctttcaaa attaccgttt
     9901 gtgttgttgt cacagtagac cggaattttt atatcgtcag attcccagat tttatcgccg
     9961 ggtttggaat tggccggaac ggcgaagggc ataatagctt ccgatttttc tacagcgccg
    10021 tttgcggtac caaaatagca gttcagcgcc agcgccgctt ttagcggcaa aaaaatcaga
    10081 cagatggcga gcaacacgcc tttggcgaat ctcatttttt attctccacc ggctgcaggc
    10141 ggcattgccg ctggcaggag agcgtgttca tacgcaatgc gccgtagtcg tccatgtagc
    10201 caagataaaa ctgcttgccg ctatagctgc ccgtgctggt ggtgaccgaa ctgaacggtg
    10261 cgaccatcgt gcttttaaag ccaggcagca cgcctttctc atcccgccca agataggcaa
    10321 tagtcagata gtatccggtc ggggttttca gggtaagctg gttattctgc tggctaacct
    10381 gtagctgttg ctcaacatga tcgcccattt ttttgcgtaa tgcggcgggt cgccagaata
    10441 gcttaatgcg actttggacg gcgacttgca ggatcgcatg gccctcgctg ccttccggga
    10501 caggcggaat ttcacgcagg ttatagtaaa agagagattc gcgatcggtg ggtaatttcg
    10561 cggtggcggc ttgtttcatg atccgaacct gcgaagtcgc ttttggctcc agtcgctgga
    10621 taggcggcag ggcgacgaga aattcatcgc ttttctctcc cttttcattt tctatccatg
    10681 aataggcgag gtaggggagt ttatcgctgc ggttttccac gcgcaggctg gttgctttat
    10741 ctttgctatt aaagatgatg cgcgtgcggt caggctgcac tgcgccgtgg ctgcacatac
    10801 tggcaaccag aagaatcaaa gggaatcctt taacaaatgt catcgtcttt tccttaatta
    10861 atgacagggt aaaatcaggc gcttagtcac atcttccaga tgttccggca gatgaatggc
    10921 acacgttttc tggtcgcccc agtgcacggt aaattgttga ttttcatcga ccccactcag
    10981 ccatgcgtga ccgttttcgc caaccatccc gacgctgaca ccgccttcag cctggcgaac
    11041 atcggcaccg agcggcggga aatgcccgtt agcatcgctg ataatgacgt tgagatcttt
    11101 cccggcgcgg gacgccagcg atttaaagcc gatggcgcct tcggtccagg tttctttcac
    11161 cacgttttcg gaaacggtaa cgccgtcagg caagtcgttc atattgaccg ccaccgtcgt
    11221 aggctggtag ctggaaacaa acggtaccac ggcaatacca aagcggttgg tgtagtcaat
    11281 attgccctga atcggaatat cgcccacgcc gtcggtgctg accatcaggc gtggttcatt
    11341 ggtggaactg cggcgatgga atgccgcgcc atgttgagtc gcggtaaacg agccgctcca
    11401 actggcactg gcggaagtgt aatcattggc ggcataggtt ccggtcagat cccattcccc
    11461 cgccgaactg aggtgttggt aattgccgct gacctgcgca ccgttatccg ggcgatcgga
    11521 ttgtattcca gccgacattc cccaactgtt tcgttcatcc agggtatcgt tccacgacat
    11581 gcggtgggtg gtagtattac tgttgttctg catgtcataa cccagtcgac cactttcccc
    11641 aatcggtaag gagatcgaaa agtaaatctg gttgtcgcga tctttatctt cataatgggt
    11701 ggtgttgaac gaggtcgaga cagaaatatc tttccagtcg ccaatgtcga cgttaaaacc
    11761 gacggtaatg ttggccgtgg tggaggtatc cgcattccac cagctctgat gcaggatgtt
    11821 ggcgtagaga ttaagattca gcagagtaat aggctgaccg aaggagaggc tgattgtttg
    11881 cttttcatcc tgcgcgtcgg cgtcattgta tttgtgatca atgtagttag cgtagctgtg
    11941 aaaatggcga tcggagaagc gataggctgc caatgagatg gtgctgttgg tcgcatcaac
    12001 ctgtttgctg tagttaaagc ggtagctgta accctgctca tcctgttggg tatcaaaatg
    12061 gctgtcggcc caggtcacgt caaacgacag cgcccccatc cagagcatat tttgcccaat
    12121 acccagcgcg ccggaacgat agtcatcgcc tgcgagaagc atgccgccat atagtgaagt
    12181 gttggagagc attccccagg agacttcatg gctaatgaag gtttcatcct cggtgtggtg
    12241 cgacatagag gaacgcgggc gaccggcagc cagtttatag cgcacctggc cctgacgcgt
    12301 caggaacggg gtagaggccg ctgaaacctg gaagttattc acccgtccat cttcttcgct
    12361 cactttgaca tccagtgtac cttgcactga ctgattcagg tcatcaataa taaatgggcc
    12421 tggtggaact tttttctggt aaatcacgcg accagaatgg cttatcgtga ccgtggcgtt
    12481 agtctgtgcg atgccgctga tttgcggcgc gtagccgcgt aactcccagg ggagcattcg
    12541 atcgtcgctg gctaacgccg caccggtata ggagaaacca tcaaaaatgt tagagctaaa
    12601 atcggtttcg cccagggtta atcgggaacc tatttgcggt aacgggcgga acaggtaggt
    12661 ccgtgaaatc gccccagatt gttcacggtt gtcgccgcta tcggactggc taagctgata
    12721 atcgctgcgc agacgccatg ccccggcgtt gaggcccgtg gtgccatagg cgttcaggtt
    12781 gttgctgctg ctgccgcttt gtgggcgata agtgctggca aacaggttgt aatccatcag
    12841 gaagccagga ataccgttat tccacgttga gggtggcgtc cagttttctg aatgccacgc
    12901 cagccatgcc tgcgggattg taatattgag ctgttgattt gcctgatcga aagtaaagat
    12961 gatttcaggg aagacactaa aatcgacaca ttcttttatt gccggtaatt ttttacgaaa
    13021 gtcagattta agtccgaaga gttcgatcag cgattcattg atacaaggaa taattttatc
    13081 gccagatttt tgccagcgaa tttgttgccc attactgatt ttattattat taaccgtaac
    13141 actaacaaag taatcgccgg gagctacgac gcctttttga ttgagcaacg aaatatcaac
    13201 gctatcccga atcgacttat cgagaacgtt aaggttaaat tcagctgtca gcgcaggagg
    13261 ggttaccatt gccagacagg aaagaacaga gagtcgataa atattcacgg catccatacc
    13321 tgaacaatat tcgcaaaagg cggctaatca gccgccttaa aaaggattat tggtaatcca
    13381 gaacgtaagt tgcgttagca gtaacggccc ccgcggtaac cgaaactgcg ttatccatct
    13441 gttccatcca ggcaaagaag tttaacgttt gttctgttga acttgccaat aagtcaattg
    13501 ttggcgcttc tgttcccaat gtaacaaatg attcatcttt gttcatgaga cgtacgccaa
    13561 cgccggtagc ctcgccagca ctgatgttac taaggagcgg ggttgcacct gtcgttttag
    13621 ccgtgctgtc gaaggtcacg cctacctttg agaccacgcc acccttgcca tcatcccagc
    13681 ctgcaatatc acagttgatt aagcgaatat tgacaggttt tttctcgcta tgatgttctc
    13741 gatttatgtg ggatgtggtt acttcaccca ggtcaatgtt catatcaagg tcatcagggt
    13801 gaatttcaca tggtgcttca ataacttcac ctttaaagtg gatttcacca gatccttcat
    13861 ctgcagcaaa aacaggtgaa gcgaatagta gagatatggc cagggcggcc agtgtttttt
    13921 gtcctttaaa catacatgaa tccttcatgg gctatg
//
LOCUS       DAZFDC010000124        13715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFDC010000124 DAZFDC010000000
VERSION     DAZFDC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.124"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            216..1274
                     /locus_tag="ACHJ48_003796"
     CDS             216..1274
                     /locus_tag="ACHJ48_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO4709268.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1284..2426
                     /locus_tag="ACHJ48_003797"
     CDS             1284..2426
                     /locus_tag="ACHJ48_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO4709269.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2419..3192
                     /locus_tag="ACHJ48_003798"
     CDS             2419..3192
                     /locus_tag="ACHJ48_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709270.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3194..4273
                     /locus_tag="ACHJ48_003799"
     CDS             3194..4273
                     /locus_tag="ACHJ48_003799"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO4709271.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4273..5229
                     /locus_tag="ACHJ48_003800"
     CDS             4273..5229
                     /locus_tag="ACHJ48_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO4709272.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5240..6448
                     /locus_tag="ACHJ48_003801"
     CDS             5240..6448
                     /locus_tag="ACHJ48_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO4709273.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6466..6933
                     /gene="terW"
                     /locus_tag="ACHJ48_003802"
     CDS             6466..6933
                     /gene="terW"
                     /locus_tag="ACHJ48_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO4709274.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7194..7523
                     /locus_tag="ACHJ48_003803"
     CDS             7194..7523
                     /locus_tag="ACHJ48_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4709275.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7510..7851
                     /gene="tnpB"
                     /locus_tag="ACHJ48_003804"
     CDS             7510..7851
                     /gene="tnpB"
                     /locus_tag="ACHJ48_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4709276.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            7897..8763
                     /locus_tag="ACHJ48_003805"
                     /pseudo
     CDS             7897..8763
                     /locus_tag="ACHJ48_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8794..10407)
                     /locus_tag="ACHJ48_003806"
     CDS             complement(8794..10407)
                     /locus_tag="ACHJ48_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO4709277.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10438..10788)
                     /gene="tnpB"
                     /locus_tag="ACHJ48_003807"
     CDS             complement(10438..10788)
                     /gene="tnpB"
                     /locus_tag="ACHJ48_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4709278.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10785..11210)
                     /locus_tag="ACHJ48_003808"
     CDS             complement(10785..11210)
                     /locus_tag="ACHJ48_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4709279.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11264..12015
                     /locus_tag="ACHJ48_003809"
                     /pseudo
     CDS             11264..12015
                     /locus_tag="ACHJ48_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            12355..12528
                     /locus_tag="ACHJ48_003810"
     CDS             12355..12528
                     /locus_tag="ACHJ48_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709280.1"
                     /translation="MSSPEQYLSSSEPASDTSLAVAPAQQNKSTIVRLRSFVCIADDA
                     PEKRVNMSSPEYY"
     gene            13548..>13715
                     /locus_tag="ACHJ48_003811"
     CDS             13548..>13715
                     /locus_tag="ACHJ48_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709281.1"
                     /translation="MEFSVIFFWVITGLITILFLKSIITDLIKNLNKTDIALYILAFL
                     SAGYTFQYIYND"
BASE COUNT         3337 a         3582 c         3536 g         3260 t
ORIGIN      
        1 gttttctgta aaaacacaaa aagaaatcct tttcagggtg ttagtttttc tatttttaat
       61 ttaaatatca atgtgttata aggaatccaa aaaacactga cgcactccat aaccacagga
      121 gttattaatc atattatgat tgacatgtag cttagacact catagactca cttctcaatc
      181 ataaatttgc atggcgttcc ggataattaa aaaaaatgaa caagaaaatt tggtttatgg
      241 aaggtttatc ctcccagcga gatattattc agggggtaaa atcatttgca caaaaaaata
      301 attttgccat taccgttttt gcctcccacc gtaacgaaag aaatgaaatc ctttccgttg
      361 ccgattattc tttgactgaa cctgaagatc ctcaaaaacg tcttcagttt atccaggaaa
      421 ccattcagac ctacggcatc caccatattc atactggccg taacagccag tggtttgaag
      481 aacaccgttc agccattgaa ccgaccggtg ccacccttac taccggtgca acgggcgtcg
      541 actggttaac tctggctgac gaaaaagtta cttttgctca gtttatggag caaaagggtc
      601 tcccggtcgt accatcctgg cgggtgaata cgctggcaga attaaagaca cacctcgcgg
      661 ccccgccgtt cactgacagc ccggtatgcg tgaagccggt gacgggtatc tatggcatgg
      721 gattctggcg ctttgatgac agtgcttcgc ctatggccgt ctttaatcat cccgaacatc
      781 gtctggtcag tccgcaacag tatattgcag cagcatcagc tgctgagtcg tttaaacccc
      841 ttgttttgat gccgtacctg ccaggcccgg aattttccgt cgatatcctc gcggataagg
      901 gcgaaatact cgcagccgtg ggacgccgta aggaaggggc tatccagtat ctggtaaacg
      961 aaggaagcgc ctgggaactg gcgtgtgact gcgcccgtgt tatgaaggcc gacgggctgg
     1021 tgaatgttca gacgcgaaac gatgtgaatg gcaacccggt gctgcttgaa accaacatgc
     1081 gtccgtcagg gggggtgggt tatacccttc acagcggtgt gaaccttcct gggttatttg
     1141 ctgcctttaa gctcggtctg atgtctgaag atatggtacg ccagagcgct aaaaacacct
     1201 tttctccggt tgcggtgaga tccattacgg atgtaattgc atacccggaa tcactctcta
     1261 accttctgaa ttaaggcgta tttatgtctg atatcctcga agatatgatt tatcgccgta
     1321 ccctctcatg cggtacgatt caggtaaccc gtgaccaggg tgaagtttcg ctcgatgacc
     1381 tgtttgacat cgctgaaaga cgtaacccga aacgcgcctt tctgtttgtc agcaaagtac
     1441 tcggcaggca cattcccgtg ccaccctcag tcatgcggca ggcctacaga cagcttgcca
     1501 gccagttccc ctcgacactg acaggacccg tactgtttat cggtatggct gaaaccgccg
     1561 ttgggcttgg ggccggtgta tttgatgaag tgcgccacca gcatcgtgaa tctgtctatc
     1621 tgacctctac ccgtcacccg gttgatggca cgttgctttg cgagttcaag gaagaacaca
     1681 gccacgccac cgatcatctg atctatctgc cagatgatga agagaaaaga cgtcgtgtta
     1741 ccaacgcacg aacgctggtt ttgattgatg acgaggcaac caccggtaat acctttatta
     1801 acctgctttc tgccctgcgt aataccggca agcttcaaca tattgaacag gttatcgccg
     1861 taacgctgac cgactggagt ggcaatgccc tgtcggagcg cacccccttg cccgtcactt
     1921 cggtttcgct tgtaagcggt cagtggggat ggaccccatt acctgatgca cctgtcccgg
     1981 acatgccgaa agtcaacgta acttcacgag gagaatggga catccagggg aaacagtcat
     2041 ggggccgact ggggatgctc gcacctgcgg ccgatctcgg ccatgaggtc tccgtccaca
     2101 agggggaacg tattctggtt ctcgggaccg gggaattcgt ctgggagccg ttcctgcttg
     2161 ctgaacggct cgaagctgcc ggagcacagg cattttatgg atcgaccacc cgctcccctg
     2221 tcgccgttgg ttatgccatt gagtccgcca tttcctttac ggataactac gggctgggca
     2281 tccccaattt tgtctataac gtcgcccacc agcagtttga ccgcattctt gtgtgtactg
     2341 agacacccgc agaaagtatt gacacgcagc ttcttaaagc gctggctgag gttgcgcccg
     2401 tcgtggagat tgttacctat gaataaaccc gttgttctga gcgaccttga cgacacgctc
     2461 tttcagaccc gtcgtaaaat ggtggacgag ctggccctgg agccattccg taccggtgcc
     2521 gttgatcgca ccctgaatcc acgaagcttt atgacggaag aacagtccat gttggtggac
     2581 tggctcctgg agcaggctga actcataccc gttaccgcac ggggaactga agaaatcagc
     2641 cgcgtacgga tccctttcca ctcctgggca atcacaacgc acggggccgt cattctcacg
     2701 cctgaaggca aacccgacga ggagtggaag gcccatatgc tcggccagct ggctccctac
     2761 caggaaaagc tgacatcgat gcagcgtctg atcactgaaa tgatggacgc aaaaggaatc
     2821 aatgcctggg caaggctgaa cttcgaatac ggtgaaacgg cggtttatat ggtgatgaaa
     2881 caccgcgaca gcacccgcct tgacgagctc aatgccattg cagatgagat agaaacggtg
     2941 tttccgaccg agggcttcta catccaccgc aacagtaata acgtggcctg gcttcccacc
     3001 ccggttgaga aagggctggc agtcagctgg ctgcttgaaa aacttcgggc tgaacgagga
     3061 gttttccccg taattggttt gggcgacagc ctgagcgatc atcgttttat gaaactgtgc
     3121 agctggtttg gcatcccgcg tcagagtcag tttgcagatg ccatttcaca gcgaattttt
     3181 ggagaaaatt aaaatgcagc cacatgaaac atttaccggc tcataccagc ccggtgacgt
     3241 ggaatttctg ctaaagccgg ttgtcattga gatgacgccg gttgagaaaa aagaagaact
     3301 gattcagtcc gggaagaaac attattcgga catgctcagc caggagccag cgcctacaca
     3361 atggcatctt gatttgtttc accgggcgct ggatcggggc gcagagaggc ttgcaaaaga
     3421 agtcacacag ctcgctattg ctctggccga acgcttcggt gatgagccca ttgtactggc
     3481 cagtcttgtc agggctggcg ttccgctcgg cgttatgctg caccaggccc tgcgtgacat
     3541 ggggaaaacc tcatggcatt acggtatcag cattatccgg gatcgtggaa ttgacggtgc
     3601 agccctcgac gtcattgaag agcggcatgg taccagcggt attgtctttg ttgacggatg
     3661 gacaggtaaa ggcgcaatta ccggagagct tgtacgcgca ctgaaagatc gcccgggcta
     3721 tcctgagcag ccgcgactgg ttgtcctggc cgatccctgt ggctgctcct ggcttgcagc
     3781 cagtgatgat gactggctca ttccttttgg catcatgggg gcgccggtat caggcctgat
     3841 ctcccggtca gtatggtcct ctgaaggatt acacgggtgc atggtttgcg agcatctcag
     3901 tgaattcgaa tgcagccgga tgcttgccga taccgtcgct catttccgta agaagttaac
     3961 accgtcatcc ctcgctcccc tgagctggaa tacggaatca gcccgggtct tatggcagac
     4021 aagtcgcgac gttatcgcgt tcctggccga tgaatttaaa gtggacagcg tcaatcgtat
     4081 taaacccggt attgctgaag caacccgggc tgtattacgt cgggtaccgg accatgtatt
     4141 tgtgcgttct attgacgacc cggacgttgc cttgcttgta gggcttgctc gtgaaaaggg
     4201 aatagttgtt acagaaatgg ggggaaccct cggccagtat cgggctgtaa ccattatcaa
     4261 gaaggtactc tgatgaaaaa tcgtctttct ccctggaatc tgggagccac gctatacatg
     4321 cctgcaacac gggaagatat tgctgatgcc gtcctgcacg ggaaaatccc gggcttgcgc
     4381 tctctggtga tttgcctgga ggatgctgtc agtgaagcag acatccccgt cgccctcaaa
     4441 aatctggagc acctgctgca cgagctcagt aacagcatga gtagcctggg taaaaatgac
     4501 tggcctctgg tgttcattcg cccccggcat gccgaaatgg gcagatggtt aaaagcgcat
     4561 tatgatcttt ccgctgtcga cgggtttgtg ctgcctaaat ttaccctcag ttcgttagct
     4621 gaatggtggg atatcatggt cgggactcac ctgtgcatga tgccaacgct ggaaacagaa
     4681 gatgtctttg acgtggttca gatgcgcgag ctggctaccc gtctggagga acatccctgc
     4741 cacgaccgca ttattgcgct tcgaatcggc ggcaacgatc ttatgaacgt tgtgtcgctt
     4801 cgccgtcccc gggacctgac gctgtatgac agcccgatgg gctacgtcat caaaatgctc
     4861 gtttcggtct tcggcccgcg tgattttgcc ctgaccgcgc ctgtatgtga gcatattgac
     4921 gatcatgccg ttatggccag agagctggct cttgatatgg cacacgggct tgttgggaaa
     4981 acggccattc acccgggtca gatagaggtc attcaaaacg cgctgatggt cactcagggt
     5041 gagcattctg acgctctgcg gatcctgaac tctacccagg ccgtgtttaa gtcgcaggga
     5101 gcaatgtgtg aacccgccac acatcgccgc tgggcggctg gcattctgga cagggctcgt
     5161 ttttatgggt tacagaacga gcaaagcgct gatggaatca gattacttac cgtgacccag
     5221 catcattaag gaaatcataa tggggttcag gttcagaaag tcgatcaaca ttattcctgg
     5281 cgttcgcctc aacctgagta acggtgcacc gagcctgagt gtcgggccga gaggtgcttc
     5341 cgtttctttt ggtagccggg ggacctatgc caatctgggc ttgcccggta ccgggctgag
     5401 ttaccgtacc cggcttgacc gggccgcgcg ttccagaggt gaaaaccgga cggcaaccga
     5461 cccggggctc agacaggcgc ttgagcagaa agccgctgaa ctcatgtcag cggtaaccgc
     5521 aatccgtaat attcacgagc tgacgccgga tccaaaaaca ggcatcagct gggcagagct
     5581 ggaagcagta tacctgcata acagaacgtc gccttttcag gttccggcac cggtgcgtcc
     5641 agaaaagcca gactaccttg tattgccgga aaagcctgcc gagagcgaag gcattagttt
     5701 tttgggtaaa tggtttgaat cggaatcagc taaagctgag cgccacgccg aaaatcttcg
     5761 ccggtggcag caggagctga ttgatgtgga gcgtgagaat acccttcgac agcaccggta
     5821 ccagcaacaa cggacggcct gggccgaaca gtatgcaaac tggaagtttg aagctgaaga
     5881 acatgaaaaa cggctcgcca cggctcaggc agatgcccgg cagcagttcc ggacagacgc
     5941 cgcgtttttc gaatcatacc tggcgggtgt gctggcagaa actgaatggc cgcgtgaaac
     6001 gcttgttgca tttgaagtaa agccggagct atcagcagtc ctgctggacg ttgatttagc
     6061 tgagattgaa gatttccctg ataagattta cggcgttaat gcccggggaa cggagctgac
     6121 ggaaaaagcc atgacgcaaa aagccgtacg cgaaaactat gcccaccacg tccatggctg
     6181 cttgttccgc ctggtcggta tcgttttaca tacgctacct ttcgacaacg tgattgtgtc
     6241 aggctttacg caacgggtca gtaagcggac cggctatctg gaggatgagt atatcctgtc
     6301 ctgcaaatgc actcgcagcc agatgtcgtc agtaaatttt gcaggcataa aacacattga
     6361 tccggttgaa gcgttaggcg atcaaccggt tattcgaaag atgagcagta ccttcatttt
     6421 tcagcctatt gaaccactaa ccctttaagc aggctaaatc ttacgatgca attaaatacc
     6481 agacaggccc ggatttttaa gcttgccaac ttactgggca ccggcaagcc cgtttccgct
     6541 gccgacatta taaccagtct ggaatgctct gagccgacat taacccgggc cctcaaagag
     6601 ctacgcgagt catattctgc ggagatcaaa tacagcaagg caggtcattc ctatcatctc
     6661 gttaatccgg gccagttgga taaaaagacc ctccgccgga tgaatgaagc gctcgcacag
     6721 aatgctgaac tgaaaaccgg tgagtccaca gggaaggtcg tactcgataa ggataaaaaa
     6781 acagccgtgt cattatcgct acgcatgcgg attctaagaa aaattgaccg tctggctgcg
     6841 ctgagtgact cgacccgaag tgaagcggta gagaagctgg ctctgcactc cgttgatgaa
     6901 ctgataaaag aatacagcgc taaaaagtcc tgacgctaaa tctcagaccg gggtgaaaac
     6961 tccggttttg tcatatttca cctctgtatt ggcgtgatgc aaaatggcat ctgaatcgat
     7021 catggtttgg catccatttc cgatcactgt ttggcatcac aatcgatcac gatttggcat
     7081 gcttccgctc attgattggc atcctggtgt cgttacgggg aagtcatctt cacagggatc
     7141 ttcagtcagt tagcattacc gacatccatt caccaactta ccggagataa tcaatgtcaa
     7201 aaccccgatg gacgcttgat cagaaaaaac atcacgttgc cgcatggcgc gccagcggcc
     7261 ttacccgtga acagtactgt gaactttacg acattccgtt taaatccctg cgccagtggc
     7321 cgcaggatgt cgcaaaagcc gaaaaacgag ccagagcacc agaaattatt cctgtttcag
     7381 tatctggctc ttccggaatg actgatggca gaccattgtc ggatgagcct gtcactctct
     7441 tcctgcccgg tggtatccgg atgtgctgcc agccttctca gttaaccgat gttttccggg
     7501 ctcttcgcca tgctgacgcc tgataacgta ttcctggtcg taaaacccgt cgatatgcgc
     7561 cggggtatcg acacactgac acagtacgtc cagaacgaac ttaacgctgc atggcatgac
     7621 ggtgctgctt ttgtcttcac caataaagtc cgctcccgca taaaagttct ccggtgggac
     7681 aaacatggcg tatggctgtg tacccgacga ctgcaccggg gaagcttccg ctggccccgg
     7741 aaaggtgatg ccacatggca tctcacacag gatgagtttc actggctggt ctttggggtt
     7801 gactggcaac aggtgaaagg gcatgacctg gcaaaatggg tataccagta agaacccgaa
     7861 gatgctcaga gcaacgcaat aactcaatga aatataatga atatccatgc gtggtgtcat
     7921 ataatccacc gcatggatat ctcgcttctc agtaccacca gtgaccctga acagcttcgc
     7981 gctctggcaa ttgccatggt gcaaaaagtc atggctgaga atgcagaact tcagaaccgt
     8041 atccgcatcc tggaagagca gatgaagctc gcacgccagc agcgtttcgg taaaaaatgc
     8101 gaatcgctgg ccgggatgca gcgttccctg ttcgaagagg atgttgacgc ggatatcgca
     8161 gaaatctcgg cgcatctgga caaactgctt cctcagaccg gggacgaaga aaaaacaaca
     8221 acacgtcctg ttcgtaagcc cttgccttca cctcttcccc gtgctgaaaa ggttatcccg
     8281 ccagctgagg aacgttgtcc tgactgtgat gcccccctgc acttcattcg cgatgaagtc
     8341 agcgagaagc tggaatatat tccggcacag gtcgtggtca accgctatat ccgcccgcag
     8401 tacagctgtc cctgctgtga gaaagtgttc agcggaaaaa tgccggcaca tatccttccg
     8461 aaaagtgctg ttgagccatc ggtcatcgca caggttgtca tcagcaaata taccgaccat
     8521 ctgcccctgt atcgccagca gcacattttc tcccgaatgg gggtggagct ccccgtcagt
     8581 acaatggcgg atatggtcgg tgttgctggc gctgcactgg ccccgctggc aaaattgtta
     8641 cgccacgagc ttctgacacg ggatgttatt catgcagatg aaacgtccct gaggctcctt
     8701 gatacccgga aaggcggtaa atcctgctcc ggctggctgt gggcttacgt cagcggagaa
     8761 agagtaagcg tcaacggagc accgtattga cgcttattta ttggtgagta ctacgttcca
     8821 tggcaggagt tcatcaactc ggttggaagg ccattctggc agtacgctca ggatatggcg
     8881 cagatacgct tccggatcga taccgttcag acggcaggtg ccgatcagcc cgtacaacag
     8941 tgctccacgc tcgccgccgt gatcgctgcc aaagaacata aagtttttct ttccgagaca
     9001 gactgcacga agcgctcttt ccgcagcatt attatccgcc tccgccagac cgtcatcact
     9061 gtaataacag agggcatccc actgattcag tacatagctg cacgcttcgc ccagtctgga
     9121 ttttttcgac agcgtaccat tcttctccac catccattca tgcagtgacg tcagtaacac
     9181 tttgcttcgc tgctgcctga cggcaagacg ctctgactcc ggtaatcccc gtatttcatc
     9241 ctcgatggcg tacagttcac tgattcgctt cagggcttct tctgccgtcg cacttttgct
     9301 gctgatgtat acatcgtgga tttttcgccg ggcatgggcc cagcacgcaa cttctgtcag
     9361 tgcaccacct tcacgttctg cactgaacag ccgatcgtaa ccgctgaacg catccgcctg
     9421 caggataccc cggaagggac gaaggtgttg taccggatgt tttccctgcc tgtctggtga
     9481 gtaggcgaac cagaccgccg gtggctctga tgagcccgca ttccggtcat cccggacata
     9541 cgtccagatg cgtcctgttt ttgccttttt tctgcccggt gccagcactt ttactggtgt
     9601 gtcgtcagtg tgaaccttgc gggtgttcat cacgtaacgg tacagggtat cattcagcag
     9661 agtcattaac tgacaacatg cgtcaaccca gttggagagt aatgcccggc tcagttcgat
     9721 gccctggcgg gcaaagattt cactctgacg atacagtggc aggtgttcgc agtattttcc
     9781 cgttaacacg cgggcaagta atccggagcc cgcgatgccg cgctctatcg ggcgggacgg
     9841 cgctggcgct tcaactatac agtcacattt tgtacaggct ttttttaccc gaacagtgcg
     9901 gatcactttc agggcgctac tcaccagttc cagctgctca gcactgactt cccccagata
     9961 atccagctca ctgccacact ccgggcaaca actttcttca ggctccagac ggtgtatttc
    10021 acggggaaga tgtgccggta acggacgacg atgacgtgac tgtcgcaact ggcggggaac
    10081 ctgcgggtca tcctcacgcc cactgtaacg atcgctttcc tgttcgcgtt gtttcagttg
    10141 ggcctcagcc tgttcaacct cacgctgcag tttttcagaa cgggtaccga acaacatccg
    10201 gcgcagtttt tctatctggg ccctcagatg ttctatttcc cgctcctcct cttcgatctt
    10261 ttcctcggca cgtgccagtg cagagcgcag gaaggcctcc gtctcttcaa ccagactcag
    10321 ttgctgatct ttctgacgga gggcttcagc ctgctcagag agcagccttt ccagctcagt
    10381 gatacgaatg aggtatttcc gactcatgac cgtttttata atccggccat gacattttta
    10441 caacattgtc agtgcattaa ggcgggatgt tttgggctga cgccagtcca gtttatcgag
    10501 gagcattgcc agctgcgagc gggtaatgga taccttaccg tcacgcaccg caggccagat
    10561 aaactggcct tcctccagac gtttggtgaa caggcacaga ccatcagcat cagcccacag
    10621 aattttaatc gtgtcacccc gtcggccgcg aaagataaac aggtgaccgg agaaggggtt
    10681 ctcatccagc acatgttgta cctgttctcc cagaccgttg aaggatttac gcatatcagt
    10741 aacgccggca accagccaga ttcgagtgcc tgatgggagc gagatcatcg tcccctcccg
    10801 gtcagttcac ggatcaacac cgtgagcagc tctggtgaag gattttccag cgtcatgtta
    10861 ccgtggcgga actcaacttt acaggaactg gcactgactg tgctttgtga aggagtggat
    10921 aaaagcggag taagagccgc cataggctct ttctgctcat caggcgttat ctcaacaggt
    10981 aataattcaa cgccagcgcc agaagaggtt gttaccggaa gacgccgcga tatacgcctt
    11041 tcgttctgcc agagcctgag ccatttgaac aggaggttat cattgatatc gtgttcccgg
    11101 gcaatacggg caacagaggc tcctggttgt gaagccagtt taaccatttg aagtttaaac
    11161 tcatttgaaa atgatctgcg gggttctgag gataatattt tctgttccat aacaggtgtc
    11221 cactagttga aaaggtgggc acctacgtta ccaatactgg cttaatggct acatacggcg
    11281 gtcagtttac gcttacgaga aagaagcggt ccgccggtcg tctgttttga ctcgcagacc
    11341 ggacgggccc tcagatatcc tgaggcctgg ctgcagggct ggtgtggcgg tacgctggtg
    11401 agtgacggat acagcgtgta caaatctctg gcggataatg accccggtat tacatctgcc
    11461 tgttgctgga gccacgcaag gcgcggtttc gcaaatctgt ataaggccag cagggagccc
    11521 cgtgcagcca tggcgctgag aaaaatcgcg ggtctttatc gcattgagaa gttcatccgt
    11581 gaacgtcctg tggaaaaaat acggcaatgg cgacaacggt attcccggcc gatagtgaat
    11641 gacctgtttg cctggcctga agagcaggag ccgtgctgtc cgcccgacgg accactaaac
    11701 aaagcgatta attatatcct gaaccggcgc gatgaactga gctgcttcct gagtgacgga
    11761 gccgtgcccc tggacaacaa tatctgcgag cgtgctatcc gcccggtggt catggggcgg
    11821 aaagcatggt tattcgccgg ttcactgatg gccgggaacc gcgcggcaca gataatgagc
    11881 cttctggaaa cggcaaaacg taatggtctg gagccgcatg cctggctgac agacgttctg
    11941 acgcgtctac cggagtggcc ggaagaccga ctggaagagt tgctgcccct tgagggattt
    12001 accttctccg ggtgagtgat acctgccatc aggtgttcgt gcagcgggcc ataacccgca
    12061 gtcgggagtt gaacgcctga cgacaggaaa tgagccagag cagcgaccac gcgggctgac
    12121 agtgagtcag aacctgaatc agcccggagc cattcccggg gctgtacttc atcacctgcc
    12181 atcagtaccc gtgtcagtac ttcgcgacct gacgcaatta cccgactcag ttcttcagaa
    12241 cccgcctccg ataccagcct ggcggtcgcc cctgtgcaac aaaataaatc aaccatcgtc
    12301 agacggtgat caatcgtatg cgctgccagt ggctcgcctg aaaaatgcgt caatatgagt
    12361 tcgccggaac aatacctcag ttcttcagaa cccgcctccg ataccagcct ggcggtcgcc
    12421 ccggcgcaac agaataaatc aaccatcgtc agactgcgat cattcgtatg catcgctgat
    12481 gatgcgcccg aaaaacgcgt caatatgagt tcgccggaat attactgacg acacggtttg
    12541 tagttacgtt ccttcggtga gtgagtacct gtgcgtcagg gtaacgcaat cagttcaggt
    12601 ttagcggggt gtcaagtcgg tttggctaac cgaccgcaga attaactgta cctttaaagg
    12661 tcgcccccga tatatctgcc tgtcagacat atcgccgatt cggtatctca ctacataaaa
    12721 ctccttaaaa aacaatgcga ttctgttggt acgaagcttt cagtaagtca gtccggagaa
    12781 gcgccagtac gatacagaat gaggttgaaa aaaacgttca cgtctggtca cttcttcaaa
    12841 tgttaaaaaa catctcgtaa agagccagtt tatgcgggtt cgttgagtag acgtgcgagc
    12901 gtcactttgt cagctttaac gctgacgaaa gtgacgcccg cacagcttca aactccacat
    12961 gcacacgcaa gcgaccaaat ttcaaatggt cttatgccgt gcgcacagtc ataagaagcg
    13021 catgcggaaa gtcagaaaat atcttaagac gcagatttta aaggcgtttt tatgccagtc
    13081 tgccgggaat acacagtagt ccggttgtcc ggaagcacca cggtaatccg aaggaaaaat
    13141 caacgtacct caatcaccgt agtgatatcc gttcccagag gctaaacgga tgtaccacca
    13201 taaaggcatc ctctttcagg ctacgaggat attgcaatca cccgaaggcg tttctctcag
    13261 attgctgaaa cattggctgg cagtcacccg aaggcgtccc tctcagactg ctggaacatt
    13321 ggctggtcgc aaaagcgaca gttaatgcaa atacaggtta acaaaaaggg aaatctgccg
    13381 tcaacccaac catatgaaca tgttgatttc attatgaatg tgatgttcta atataaatta
    13441 atcagtaact tcataaaaag aatataagaa acagataatg catgtattta cacacatatc
    13501 agcacaatga tagattacca ccccagaaac acgataaagg tataattatg gaattttccg
    13561 ttattttttt ctgggttatc actggtctta ttaccatctt attcttaaaa tcaataataa
    13621 ccgatctaat aaaaaaccta aacaaaacag acatagcgtt atatatattg gcatttttat
    13681 cagccggata tacattccag tatatttaca atgac
//
LOCUS       DAZFDC010000125        13593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFDC010000125 DAZFDC010000000
VERSION     DAZFDC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.125"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..535
                     /locus_tag="ACHJ48_003812"
     CDS             <1..535
                     /locus_tag="ACHJ48_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO4709282.1"
                     /translation="FSNANLTNCKISNANFSNAKFYNTNCTGANCSNILFDYAWFDNT
                     IFIKTLFKNTCFYNVRAKNVYLEGAYLNNDNIVNQANNSTEKQSIDSTDKQANDSTVQ
                     QSIDSTVQQANDSTDKQANDNIDKQVNDSTDKQAKNSTEQQDSNSFNQARLKKEVNRR
                     FSIPGLTSYQPTYIVEE"
     gene            801..1643
                     /locus_tag="ACHJ48_003813"
     CDS             801..1643
                     /locus_tag="ACHJ48_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO4709283.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            1711..2889
                     /gene="yjiJ"
                     /locus_tag="ACHJ48_003814"
     CDS             1711..2889
                     /gene="yjiJ"
                     /locus_tag="ACHJ48_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4709284.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            3100..8341
                     /gene="iatD"
                     /locus_tag="ACHJ48_003815"
                     /pseudo
     CDS             3100..8341
                     /gene="iatD"
                     /locus_tag="ACHJ48_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
     gene            complement(8498..9093)
                     /gene="kptA"
                     /locus_tag="ACHJ48_003816"
                     /pseudo
     CDS             complement(8498..9093)
                     /gene="kptA"
                     /locus_tag="ACHJ48_003816"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            9298..9981
                     /gene="yjiH"
                     /locus_tag="ACHJ48_003817"
     CDS             9298..9981
                     /gene="yjiH"
                     /locus_tag="ACHJ48_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO4709285.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9978..10439
                     /gene="yjiG"
                     /locus_tag="ACHJ48_003818"
     CDS             9978..10439
                     /gene="yjiG"
                     /locus_tag="ACHJ48_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO4709286.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10452..11624
                     /gene="iadA"
                     /locus_tag="ACHJ48_003819"
     CDS             10452..11624
                     /gene="iadA"
                     /locus_tag="ACHJ48_003819"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO4709287.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11746..12600
                     /gene="hypT"
                     /locus_tag="ACHJ48_003820"
                     /gene_synonym="qseD"
     CDS             11746..12600
                     /gene="hypT"
                     /locus_tag="ACHJ48_003820"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO4709288.1"
                     /translation="MTLEKCRNFSQAAVSRNVSQPAFSRRIRALEQAIGVELFNRQVT
                     PLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRKIKIAAAHSLSLGLLPSIIS
                     QMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLLEAPFDHIRLFESQLFPVCA
                     SDEHGEALFDLVQPHFPLLNYSRNSYMGRLINRTLTRHSELSFSTFFVSSMSELLKQV
                     ALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAYAYRMNTRMNPVAERFWREL
                     RELEIVLS"
     gene            complement(12593..12985)
                     /gene="iraD"
                     /locus_tag="ACHJ48_003821"
     CDS             complement(12593..12985)
                     /gene="iraD"
                     /locus_tag="ACHJ48_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO4709289.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSTLPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12982..13065)
                     /gene="idlP"
                     /locus_tag="ACHJ48_003822"
     CDS             complement(12982..13065)
                     /gene="idlP"
                     /locus_tag="ACHJ48_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO4709290.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(13181..13324)
                     /gene="ytiD"
                     /locus_tag="ACHJ48_003823"
     CDS             complement(13181..13324)
                     /gene="ytiD"
                     /locus_tag="ACHJ48_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO4709291.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         3602 a         3356 c         3236 g         3399 t
ORIGIN      
        1 cttttcaaac gctaatttaa caaattgtaa aatctctaat gcaaactttt cgaatgcaaa
       61 attctacaat actaattgta ctggtgcaaa ttgttcgaat atcctatttg actacgcatg
      121 gtttgacaat acaatattta taaaaacgct ttttaaaaat acctgttttt acaatgtcag
      181 agcgaaaaat gtctatcttg agggagcata tctgaacaat gataatatcg tgaatcaagc
      241 caataacagt accgagaaac aatccattga cagtaccgat aaacaggcca atgacagtac
      301 ggtgcaacaa tccattgaca gtacggtgca acaagccaat gacagtaccg ataaacaagc
      361 caatgacaat atcgataaac aggtcaatga cagtaccgat aaacaagcca agaacagtac
      421 cgagcaacag gacagtaaca gttttaatca agcccgttta aagaaagaag tgaataggag
      481 attttccatt ccgggtttaa cgtcttatca gccaacatat atagttgaag aatagttcat
      541 taatcaccca aacagagtgg gtatggcaga cgatatagat taacatttaa acgttaaccg
      601 cctcatctat cattcataga tgaggcgcat ttatcagtcg atgtaagctg aaaatgttct
      661 gccatccaga gacaaccgcc gccccctgcg gcgtttacta tttcatcgcc cgcgttaaga
      721 attcattaag cttcatccgt atactcctta tctgtcgcac tgaatttgac gagaaaaaag
      781 caataataag gagcaataag ttgaaaaagc gcatcacctt tatcgttttt ctttgcgcca
      841 tcgtggcggc aagcctgttt tttgtgcaat cctgcgtccg taaaagccag catgtcgcag
      901 ggtttcagaa ttatcaggcc actatcgatg gtaaagagat tacaggagta actaaaaata
      961 tctcctcact gacctggtca gcgcagagca ataccttatt tagcactatc aacaaacctg
     1021 caaccatcgt tgaaatgacg accgagggcg acttgatccg caccattccg ctcgattttg
     1081 ttaaggatct cgagacgatt gaatacattg gtgataataa atttgtcatc agtgatgaac
     1141 gtgactacgc catttatgtg atttcactta acgctgactc ggaagtgagc atcctcaaaa
     1201 aaattaaaat cccattgcag gaaacaccaa ctaactgtgg ctttgaaggg ctggcttact
     1261 cctctcagga ccataccttc tggtttttta aagagaaaaa tccgatagag gtctacaaag
     1321 taaccgggct attgcgtagc gatgagttgc atatcagcaa agataaaacg ttgcagcgtc
     1381 agtttacttt agacgatgtt tctggcgcgg aatttaaccc acagaaaaat acgttgctgg
     1441 tgttgtcaca tgagtcacgc gccctgcagg aagtgaccgt gagaggtgac gtgatcggcg
     1501 aaatgtcgtt aaccaaaggt aagtatggcc tttcacacaa catcaagcag gcggaaggga
     1561 tagcgatgga tgatagcggc aacatctata tcgttggcga accgaatctt ttttatcgct
     1621 ttacgtcgac aaaatcacga taacagtctg gcaggttctc cttttttgtt attaacttgt
     1681 taactcaaaa taatacaaac caggcagaac atgaattcga catcgcatcc cgtagaacgt
     1741 ttttctttca gcaccgcgtt attcgggatg ctggttctga ccttaggtat gggtttaggc
     1801 cgctttctct atacgccgat gctgccagtc atgctggcgg aaggcgagtt ttcatttagc
     1861 gaactctcat ggatcgccag tggtaactat gccgggtatc tggcggggag cctgctgttt
     1921 tcattcggcg cgtttcattt accctcacgc ctgcgcccgt tcctgttagc ttccgccctc
     1981 gcaaccggat tattaatcct cgcgatggcg tggctgccgc cgtttcttct ggttttcatc
     2041 attcgctttc tggcgggagt cgccagcgcc gggatgttga ttttcggctc aacactcatc
     2101 atgcaacata ctcgccatcc ctttgtcctt gcggcgctat tttctggtgt tggcgtcggc
     2161 atcgctctgg gtaatgaata tgtgctggca ggcctgcatt ttgccctctc ttcacaaacg
     2221 ttgtggcaag gtgccggagc actttctgcc attatattgc ttgctctggc gctgctcatc
     2281 ccgtcgaata aacacgttat cccgccagcg ccattggcaa aaatcgcgca acaacccatg
     2341 agctggtggt tactggcgat tctgtatggt ctggcgggtt ttggttatat catcgtcgcc
     2401 acctacctgc cgctcatggc gaaagacgcg ggccagcctg tgttgacggc tcacctctgg
     2461 acactggttg gcttgtcgat tgtcccaggt tgctttggct ggctgtgggc agccaaacgg
     2521 tggggagcat taccttgcct gaccgcgaat ttgctggtgc aggcgatctg cgtgctgtta
     2581 accctcgcca gcagctctcc tttattactc atcatcagca gtattggttt tggcggcacc
     2641 tttatgggaa cgacctcgct ggtgatgacc atcgcccgcc agcttagcgt gccgggaaat
     2701 cttaaccttt tgggctttgt gacactcatt tatggtatcg ggcaaattct tggcccggcg
     2761 ctgaccagta tgctcggcaa cggaacgtcg gcgctcgcca gcgccacact ctgcggcgca
     2821 gcggcgctat ttatcgcagc attaatctgc gggatgcaaa tattcaaatt gcatacgaat
     2881 tattcttaag taatttaact aatttaactt ttcagcacat cacgtaaaat aattaaaata
     2941 tcctttttct ttggaggtac tcagccaccg ccggtattat tcctggttta gcaattccac
     3001 ggctaattca ttatttctgg tcatattata gtcaggatcg cattatatct ccgtgctaga
     3061 aaaaacaagt ttaattctgt tttttcacgg aaggtcacta tggcttcgac aaatgcgcat
     3121 caaataaaaa ataatgatca aaactcttta tgtggtctgg gtgacaaaat acgccgcctg
     3181 accgccggag tttgtttatt tacacaattt tttttccccg tcatggcgac ggcacaaaat
     3241 gtggtacatg ctaaacctca gacgacagtt tcatctgccc ccccctctac tagaaaataa
     3301 tactgtgcct tacacgcttg gtgcgctgga atcagcgcaa agcgttgcta atcgcttcgg
     3361 tatttcactg gaggagcttc gtcgtcttaa tcagttccgt acttttgctc gcggctttga
     3421 taacgtgcgc cagggtgaag aactggatgt tccggcaaca acctcgcaga aaagccatga
     3481 gcaacaaaat gccgtaccgc ctgcgaatgg cgaaaacacg ctggagaatc aaatagccag
     3541 cacctcgcag cgagttggca ctctgctttc acaagatatg aatagtgagc aggccagcgg
     3601 catggcgcgt ggttgggcgt cttcagaagc ctcaggcgcg atgactgatt ggttaaataa
     3661 ctttggtact gcgagaatct ctctgggtgt ggatgaagat tttagcctga aaaattcgca
     3721 attcgacttc ctgcatccgt ggtatgacac acctgattat ctgctcttca gccagcatac
     3781 ccttcaccga acagacgatc gtacccagat caacaccggt ttgggctggc gtcatttcac
     3841 ctccagctgg atgtcaggca tcaacctttt ttttgaccac gacctgagcc gctatcactc
     3901 ccgcgcaggg cttggcgcag aatactggcg tgattatctg aagttgagca gcaacgctta
     3961 tatcggcctg accggctggc gtagcgcacc agaattggat aacgacttcg aagcccgccc
     4021 ggccaacggc tgggatttac gcgcggaagg ctggttacct gcctggccac aactgggggg
     4081 aaaactggtc tatgaacaat actatggcga tgaagtggcg ctgtttgaca agaatgatcg
     4141 tcaaagtaac ccccatgcta ttaccgcagg cctcaactat acccccttcc cgcttctgac
     4201 tctcagtgcg gaacagcgtc aggggaagca aggtgaaaat gacacacgtt ttgccgttga
     4261 tctgacctgg caacccagca gttcaatgca gaaacagctt aatccggacg aagtggccgg
     4321 acggcgcagt ctggccggta gtcgttatga cctgattgat cgcaacaaca acatcgttct
     4381 ggaataccgc aagaaagagc tgattcgcct gagtctgctg gatccggtga aagggaagtc
     4441 tggagaaata aaaccgctgg tttcctcgct acagaccaaa tatgccctta aaggctataa
     4501 catcgaagcc gctgcgctgg aagctgccgg aggtaaagtc agcacgtctg gaaaagatat
     4561 cacggtcacg ctgccaggtt accgcttcac taacacccca gaaaccgata atacatggtc
     4621 gatagacgtt accgccgagg atgtaaaagg taacctgtca cggcatgaac aaagcatggt
     4681 agttattcag gctccgacat taagccagaa agattctctg ttatccgtca atccgctaac
     4741 cgtggctgca gataaaaaat cgacgaccac attgaccgtt actgcgcacg attccgacgg
     4801 aactccggtg ccggggctgg cgctgcaaac ccgcagtgaa ggcgttcagg atatcaccct
     4861 gtctgactgg acagataacg gtgatggtag ttacacacag atactgaccg ccggaacgac
     4921 atcaggttca gtaacactga cgccgcaaat taacggtgag agtgcggtaa aagaatccat
     4981 cgtcgttaat atcgtccctg ttgtctcatc ccgcgaccat tcatcaataa caattgataa
     5041 cgtatcgtat tatgccggag acgacatcaa ggttagggtg gaactgaaag acgatagcaa
     5101 tcaaccggtt gcatatcaaa aagaggaatt ggtaaaagcc gttactgtcg aaaacagcaa
     5161 acctggcgcc acgattgtct ggcacgaaga gcagccgggg gtttatgccg cgaattatcc
     5221 ggcctataag caagggactg cactaagggc acaacttagc cttcacaact ggaatgctcc
     5281 actgcaatcg catatttata acattgaggc aaaccagaat aaggctcgcg ttgccacatt
     5341 atcagcgaca aataatgacg tttacgccga taaaaagaca tttaataccc tcacgatcaa
     5401 cgtcactgat gagagtgata atcccctgac aaatcatcag gtcaccttta agaatgaaaa
     5461 aggaagcgcg gagtttgtcg aaccgccgca gcaaaatacg gatgcatatg gtgttgccac
     5521 aataaacatg gtaagtcagg ttgcggaaga aaatacgatt agcgccacgc tgccaaatgg
     5581 tttttcacaa cggataattg cgaaattcgt tagcgattcg agtacgccaa aattcaaaca
     5641 actggttgcc gatccagata ccattattgc tggcaacagc cagggcagta ctctgaccgc
     5701 catcatcaca gactttcata acaacccgtt aaaagatatg aaagtgaatt ttgtggcacc
     5761 tggtggctcg caactggaca acacgaccgc cacaacagac cagtccggta ttgtgcgggt
     5821 gcacctgacc agttcaaaag ctggtagcta ttccgtcgat gcctcgcttg aggtggataa
     5881 aaatattcac cagtcggtca cgatcaccgt ggtcccaaac agggaacaat cggtaatgac
     5941 cttgaatgcc gggtcgggca gtgcgatcgc taacaataca aatatcgtta ccctgactgc
     6001 cagtgtgaaa gatgtttatg gacacccgtt gccggatgag gatgtgaaat ttaccttgcc
     6061 agcctccatg accgggaact tcacgctaag tagtgaaacc gcccgcaccg atgcaaacgg
     6121 tgatgccgtg gtcacattgc gaggcacaaa agcgggtgag tttacagtta cggcgacgct
     6181 gaccagaaat aataccgttg cttatcagca agtcactttt attggggata caaacagtgc
     6241 gcagctccag ccgctgactg cctcattaaa ttccattgtt gcgggtaaca gtacggggag
     6301 taccctgacg gcaacgatcc tggacgctta ccaaaatccg cttaaagacc agttggtcac
     6361 tttccagagt aacgatgtca ctctaagcga aacagaagtc accaccaata cgctgggtca
     6421 ggcgacggta acaatgacca gcaatattgc cggacaacat aacgtcgtgg tgagccggaa
     6481 agcgcaagct tccgataata aaacgtttag tttatcagtg ctaccggatg aaagttcggc
     6541 gaaggtaata agtataaccg gagccgaaaa aacgataacg gtgggcgaaa acatcacgct
     6601 acggatactc gtccaggacg cgtttaacaa tgtaatcgcg ggtcaacgcg tcagattaag
     6661 tgcgcagcca acaactaaca ttacgatagg cgatacggct tacaccgata ataacggtta
     6721 tgcgtacgtt aaccttctca gcacccaacc tggggtttat caggtgacgg caacgctgga
     6781 caataacagt agtagtaagg ttgacgtgaa tgtggcaaat ggcaaactcg agttaacatc
     6841 atcgaaacca gaaactacgg tccataatag tgagggtatt acgctgaccg caacggcgag
     6901 aaatgcgcgg ggtgaattga tgccagggca aattatcacc tttagcgtaa cgcctgaagg
     6961 tgcaacgcta agcaatacag gggaagtcct tactgaccag tcaggtcagg ccaaagtgac
     7021 gctgaccagt gacaaagtga atgtctatac cgttacggcc ataatgggca aagatgttcc
     7081 cgttcagagc caggtaacgg ttgcggttaa ggcagatgct aaaacggcac atgttgtgag
     7141 cgtcgtggct tctcctgaca ccatcaccgc cgacggcatc gatagcagca ccatcacttc
     7201 acgagtagaa gatgattacg gattcccggt tgaaggtgtc gatattagtc atggcttaga
     7261 caccaaaggc agcccggtag ttaatattcc aactacgcgt accgatcagt ccgggcaagt
     7321 cacggcgaca ataaccagta cattggcaga aaccttaaca gtcaatgtgc aagttcctgg
     7381 cacagccaac caatccgcaa ccattacatt ggttgccggc acggccgatg aaagtaagtc
     7441 aattttgaaa tccgatgttg acactctgaa ggctgactac cagcagagcg caaaacttac
     7501 gctaacattg caagacaagt acggtaaccc gatagtgacg tctgatcatc tggaatttgt
     7561 ccagtcaggc cccttcgtga actttctcaa gttgagcgat attgattaca gccaaagaaa
     7621 ttatggcgag tacaccgtga ctgtcactgg cggaaaagag ggaacagcga cactcattcc
     7681 catgctgaac ggggttcatc aggcaaactt aagcatatcg ctgaatctca tccaatcgat
     7741 aaaagaaatg tccggtcatg tcactgcaaa caaccatacc ttctccacgg ctaaattccc
     7801 gagcgaaggc tttgcaggag cgtattacac actcaacaat gataactttg aagcgggtaa
     7861 aaccgttgat gattatatgt tttcaagttc acagggttgg gtgtctgtcg atgcttcggg
     7921 taaagtttct ttcgcaaata tcggcgatca aacgtcagtc acaataagcg ctgttccccg
     7981 acaaggaggt acaacctacc agaccttaat taagctgaaa ggctggtggg tgaataatgg
     8041 aaatcatacc aatatctggc tagctgccaa tgcgctctgt catgctaaaa atgatggata
     8101 taatcttcct ggcatcacac atttgacgtc tggcgaaaac aaacgcacgc agggatcact
     8161 gtatggtgaa tgggggaacg ttggagcgtt ttccagtaat tcgcaattta caccgggagc
     8221 ttactggaca agtgaatctg atgattacag tcggcactac tatgtgcaga tgctaaccgg
     8281 tatgaccgga agcgacgctg attccagccc ccaactgacc gcctgccgta aatcacttta
     8341 atttcagcag ttgttattaa aggataccct tttacgaggg tatcctttca gactgataac
     8401 aaacgctaca ttgccggatg tgctgcgctt atcaggccta tataacccct gcaatatatt
     8461 gaatttattg tttttgtacc ccagccggca taaacgacta ctcaagaaac tcaacagcaa
     8521 ccgttgatgt cagccagacc ccgttttccg cttgccagaa agggatgcct cgtttcgcca
     8581 tttcctgtgc tttgacggtt aagacaaccg gcgatccgtg tcttgctccc actttgcgtg
     8641 ccgttgcttc atcagcagag agatgcacgt aatgacgatc ccctgcaatc agcccctgtt
     8701 tttttatctc gtcaaggaaa cggcttgccg taccgtggta gagaaactgt ggcggtgttt
     8761 tttcggcaaa ggaaatagcg acctgcgaag tcgaatgccc ctgaaccgcg cggatgcacc
     8821 taccatcact ggaataacta aaacgctttt atcgctggtt gcgaccacgg tatccagcaa
     8881 tgcacgggtt aatcgcttgc ccgctttttg ggcacaaagt atgagcttat cgatattcgc
     8941 ccatccttca cggtccagta cgataccaat cgcctccggt ttgtgtcgga ggacaaaact
     9001 taaaaacttg ctcgtatccg ccagctcttt ttcgttgtac tttgccatcg cgttttcgtc
     9061 cctggatttt ctgtttttat ttaccaatgt cattgtcact gggtttatga attttctgca
     9121 actcaatcct gcgcagaacc ttttccacac agcgtaattt ttcccctatg caaaaaacgc
     9181 atagccagaa acatacttgc attttcccca tacccacatg gctgataagg tttttaagtt
     9241 ccaggaattt tcctggctca agcaacacac aaacacaaaa aaacaatggg gattgttatg
     9301 acacaacaag gggatgctgt agccggggag ctggcgacag aaaaagtggg cattaaaggg
     9361 tatctggctt tttttctcac cataatcttt ttttccggtg ttttttccgg aacagacagc
     9421 tggtggcgcg tttttgattt ttccgttctg aacggatcgt ttgggcaact gcctggtgcg
     9481 aatggagcca ccacctcatt tcgcggcgcg ggtggcgcgg gggctaaaga tggctttctc
     9541 tttgcgctgg agctggcacc gtcagtcatt ctctctctgg ggattatttc aattaccgat
     9601 ggtcttggtg gattacgcgc cgcgcagcag ttaatgacac ctgtgctaaa accgttgttg
     9661 ggcattccag ggatctgctc gctggcgctg atcgctaact tgcaaaacac tgatgccgcc
     9721 gccggtatga ccaaagagct ggcgcaggaa ggtgaaatta ccgaacgcga taaagtgatt
     9781 ttcgccgcat atcaaaccag cggtagcgcc atcatcacca actacttctc ttccggcgtc
     9841 gcggtgttcg cctttctggg cacgtccgta atcgtacctt tagccgtcat cctggtgttt
     9901 aaatttgtcg gagccaacat tttgcgcgtc tggcttaact tcgaagaacg tcgcaaccca
     9961 acgcaaggag cgcaagcatg acaactcagg tccgcaaaaa cgtcatggat atgtttatcg
    10021 atggcgcgcg ccgtggcttt actatcgcca cgaccaacct gctgccaaac gtggtgatgg
    10081 cgtttgtcat cattcaggcg ctgaaaatta ccggcctgct cgactgggtg gggcatatct
    10141 gtgaacccgt aatggcgctg tggggattac ccggtgaagc ggcaaccgtc cttttagccg
    10201 cattaatgag tatgggcggc gcagtcggtg ttgccgccag tctggcaacc gctggcgcat
    10261 taaccggtca cgatgtcacc gttttactgc ccgcgatgta tttaatgggc aacccggtgc
    10321 aaaatgtcgg tcgctgcctg gggacagccg aagtgaatgc caaatattac ccgcatatca
    10381 ttacggtctg cgtgattaac gcattgctgt cgatctgggt tatgcagctt attgtttaac
    10441 aaggagttac catgattgat tataccgcag ccggttttac cctgctgcag ggagcgcatt
    10501 tgtatgcgcc ggaagatcgg ggaatttgcg atgtcctcgt cgctaacggc aaaattatcg
    10561 ccgttgccag taatatccct tctgacattg taccggactg cacggttgtc gatctcagcg
    10621 ggcagatcct ctgcccaggt tttattgatc aacacgtcca tttgattggc ggtggcggcg
    10681 aagcaggtcc cacgacgcgc acgccggaag tggcgctaag tcgcctgacg gaagcgggcg
    10741 tcacgtcagt ggttggtctg ctgggcaccg actctatctc tcgccacccg gaatccctgc
    10801 tcgccaagac ccgtgcgctc aatgaagaag gcatcagcgc ctggatgctg accggcgctt
    10861 atcatgtccc ttcccgcacc attacgggtt ccgtggaaaa agacgtggcg attatcgatc
    10921 gtgtgattgg cgtgaaatgt gccatctctg atcaccgttc tgccgcaccg gacgtttatc
    10981 acctggctaa tatggcggca gaatcccgcg ttggcggttt gctcggcggt aaacctggcg
    11041 tcaccgtgtt ccacatgggc gacagtaaaa aggcattaca gcctatttat gacctgctgg
    11101 aaaactgcga tgtgccgatc agcaagctgc tgccgaccca cgttaaccgc aacgtaccgt
    11161 tgtttgagca ggcgctggag ttcgcgcgca aaggcggcac catcgatatc accagcagca
    11221 ttgacgaacc ggtcgcccct gccgaaggta ttgtccgcgc cgttcaggcg ggtattccgc
    11281 tggcacgcgt caccctcagc tccgacggca acggtagcca gccgttcttc gatgatgaag
    11341 ggaatttaac ccatatcggc gttgcaggtt ttgaaacgtt gctggaaacc gtgcaggtgc
    11401 tggttaaaga ctatgatttc agtatcagcg atgccctgcg cccgctcacc agtagcgtag
    11461 ccggtttcct taacctgacc gggaaaggcg aaattctgcc aggcaatgac gcagacttac
    11521 tggtcatgac gccagaattg cgcattgagc aggtatacgc tcgcggcaaa ctgatggtca
    11581 aagacggcaa agcctgcgtg aaaggaacgt ttgaaacggc ttaaacggtt gtcagccgcc
    11641 ctaatggacg tagaatgccc ccaagggcgg ctgacagagt aaaacgtaat ggatgactgt
    11701 ggtgcggttt tgcataatat tgaaaccaaa tggctttaag attttctgac cctggaaaaa
    11761 tgccgcaatt tttcccaggc ggcagtcagt cgtaacgtct cgcaaccggc attcagccgc
    11821 cgcatccgcg cgctggaaca ggcgattggc gttgaattgt ttaaccgcca ggtgacgccg
    11881 ctgcaactct cagaacaagg gaaaatcttc cattcgcaga tccgccatct gttgcaacag
    11941 ttagaaagca accttgcaga gctgcgtggc ggcagcgatt acgcgcaacg taaaatcaag
    12001 atagccgctg cacactctct ttccctcggt ctgttaccgt ccattatcag ccagatgccg
    12061 ccgctcttta cctgggcgat tgaagccatt gatgtcgatg aagcggtcga taaactgcgt
    12121 gaaggacaaa gtgactgtat tttttccttt cacgacgaag atttgctgga agcgccgttt
    12181 gaccacattc gcttatttga atctcaattg ttccctgtct gcgccagtga cgaacatgga
    12241 gaagcacttt ttgacctcgt gcagccacac tttccgttac tgaattacag ccgcaactcc
    12301 tacatggggc gattgattaa tcgcaccctg acgcgccaca gtgagttaag tttcagcacc
    12361 ttttttgtct cttcgatgag cgagctttta aagcaggttg ccctcgacgg ctgtgggatt
    12421 gcctggctgc cggagtatgc catccaacaa gaaattcgca gcgggcagct cgttgtgctg
    12481 aatcgggacg aactggtgat cccgattcag gcctacgcat accggatgaa tacccgcatg
    12541 aatccagttg ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattgt gcttagctga
    12601 cattctcaag cgtcgcactg cgccaaccgc cattatccca gaccagatca aacgttagcg
    12661 gttcatcgtc gtggtacagc attatctcaa ggctgatcgc cagagtgtgg cagtacgctt
    12721 ctttcaaatt aacctgcaac gccgcaatcc gtggctcatg aaataacagt gcagaacgca
    12781 gctgacggca ataccagctc tggagatgtt tgtcgctcgg gttatgcact tgccactcgg
    12841 gcatgcctga aacaagtaat gacggtaatg tcgaatgagg agaattaaaa agagtgagga
    12901 gatcgctgca taccgattta cgcagcaatg acgttttatt gcctgaaatt tcaggtaaaa
    12961 cagcctgaag tgattggcgc atcattttgc gcactcctga cgtttagcaa catatgcggc
    13021 ttgccctgaa caccgggcac cactgtattg atgctcattt tccatagcca ttcccttttg
    13081 ttcattacat ccatgtcagt ctgagacagg cctgaatcct gtcagcaaag tataaaacct
    13141 tacttgccta tgaatatcta ctgacttttc aggaaaaaag tcatgatttg ataaaaatca
    13201 tcaaactcaa aaaaaaaccg ctagtatatt ctgaatattc cgaatagtta cgtagatgaa
    13261 aatacttgtc accgctgcta cttaattccg gtgggaaatt atttatttcc gcataatcag
    13321 ccatgtatta tttgaaacaa ttagtttgta aataatataa attgataaca tatcaaatac
    13381 atcaaaaaca ccattcacag gcattattaa tatagacctc aatatagcaa catcaaattc
    13441 caattagtaa taagatatga ttttaaattg catgcaaata attttaaatg agcgtttttt
    13501 gatactttag agtgtttcct accctttaat atcactgtgt agagttgata tcactacaac
    13561 agaaacatgc atgtcatttg tttacttctc ttt
//
LOCUS       DAZFDC010000126        13415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFDC010000126 DAZFDC010000000
VERSION     DAZFDC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.126"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(146..298)
                     /locus_tag="ACHJ48_003824"
     CDS             complement(146..298)
                     /locus_tag="ACHJ48_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4709292.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(562..1296)
                     /gene="cysH"
                     /locus_tag="ACHJ48_003825"
     CDS             complement(562..1296)
                     /gene="cysH"
                     /locus_tag="ACHJ48_003825"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4709293.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKNGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1370..3082)
                     /gene="cysI"
                     /locus_tag="ACHJ48_003826"
     CDS             complement(1370..3082)
                     /gene="cysI"
                     /locus_tag="ACHJ48_003826"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO4709294.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3082..4881)
                     /gene="cysJ"
                     /locus_tag="ACHJ48_003827"
     CDS             complement(3082..4881)
                     /gene="cysJ"
                     /locus_tag="ACHJ48_003827"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO4709295.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGVVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQEEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5200..5562
                     /gene="queD"
                     /locus_tag="ACHJ48_003828"
     CDS             5200..5562
                     /gene="queD"
                     /locus_tag="ACHJ48_003828"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO4709296.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5640..6911
                     /gene="ygcN"
                     /locus_tag="ACHJ48_003829"
     CDS             5640..6911
                     /gene="ygcN"
                     /locus_tag="ACHJ48_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO4709297.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDCATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDTALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPVLLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWNLAGDVIRSVRCL"
     gene            6902..7162
                     /gene="ygcO"
                     /locus_tag="ACHJ48_003830"
     CDS             6902..7162
                     /gene="ygcO"
                     /locus_tag="ACHJ48_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO4709298.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQMVQRLINACPVGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7179..7754
                     /gene="ygcP"
                     /locus_tag="ACHJ48_003831"
     CDS             7179..7754
                     /gene="ygcP"
                     /locus_tag="ACHJ48_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO4709299.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7902..8762)
                     /gene="ygcQ"
                     /locus_tag="ACHJ48_003832"
                     /pseudo
     CDS             complement(7902..8762)
                     /gene="ygcQ"
                     /locus_tag="ACHJ48_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(8759..9538)
                     /gene="ygcR"
                     /locus_tag="ACHJ48_003833"
     CDS             complement(8759..9538)
                     /gene="ygcR"
                     /locus_tag="ACHJ48_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO4709300.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAHGNSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRIRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9516..10852)
                     /gene="ygcS"
                     /locus_tag="ACHJ48_003834"
                     /pseudo
     CDS             complement(9516..10852)
                     /gene="ygcS"
                     /locus_tag="ACHJ48_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10946..12400)
                     /gene="ygcU"
                     /locus_tag="ACHJ48_003835"
     CDS             complement(10946..12400)
                     /gene="ygcU"
                     /locus_tag="ACHJ48_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO4709301.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVNCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12470..13255)
                     /gene="ygcW"
                     /locus_tag="ACHJ48_003836"
     CDS             complement(12470..13255)
                     /gene="ygcW"
                     /locus_tag="ACHJ48_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4709302.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
BASE COUNT         3145 a         3651 c         3363 g         3256 t
ORIGIN      
        1 ttatgaataa attaatgatt ttcatttgaa attcataatg ataaatagag actatatatt
       61 cacataaaaa atataaattt aaaaaagggt acaggagacc aaaccatcac aattcaatac
      121 gcggggtttt acccccgcgt ggtggctact tcttcgattc gtaagcgaga actgccttaa
      181 actccatacc acgctctctg atactcaact cacataacga gtcgcctacc atgagcgtga
      241 atcccagtac tgtacaacac agtactatga ttgccacaag ggcatatttt gttagcatgt
      301 cttgcctccc tgcaaagaag agactaacat ccgaattgct acgttcgaac gcgagcctca
      361 ggttgataga aatatcgcct ggggcttttg tccatctgga acctcgcgaa tgcttaacgc
      421 cagacagcct caagcacccg acgccattct atacctgata attcttccag cggcgttcac
      481 gtttaaaatc aggaatgctg ctcgcaaaat aaaaacgccc ccatcattac tgacagaggc
      541 gtttaattta tccggcaata tttacccttc gtgtaaccca cattcccttt ttaagccaaa
      601 gaaacgcgtt tcttcttccg ccatgccggg ttcccattta cgggttgtat gggtatcgcc
      661 caccgataaa tatccttcgt cccataatgg gtgatatttc aggccattct tttgcagata
      721 ctgatatatc gttcggttat cccagtcgat aatcggcagc actttaaata cgccacgctg
      781 aattgccagc accggtaaat tggcgcgact gccggattgt tcacggcgca ggccagcaaa
      841 ccaggtttgc gcgttaagtt ctttcagcgc ccggttcatc ggttcgactt tgttgatgtc
      901 attgtacttt tcaatgcctt caacgccctg ttcccacagt tttccgtagc gtgcttcctg
      961 ccaggctgcg ctttcggtag cacggtacac tttcaggttg agcttgagtt tgtccgttaa
     1021 ctcgtcaata aagcggtagg tttccgggaa caagtaaccc gtatcggtga ggatcaccgg
     1081 aatatccggg cgaatttgat tcaccagatg caggctcacc gccgcctgaa tgccaaagct
     1141 ggaagaaagt acatattcac cgggcagatt atccagcgcc caggctacgc ggccttcagc
     1201 gtccagtttt tccagttggg cgttagtttc cgccagcgcc agaatgcgat ctaccttcgg
     1261 cagttcgttc agggcgttta gatcgagttt ggacatagat tcctcactgt ttgccttgcc
     1321 tgatgcgacg ctcacgcgtc ttatcaggcc tacaagaccg ggctgatggt taatcccaca
     1381 aatcgcgcgc cggatcgagc accgggcgaa tgatgcccgc acgcaccgta aagtcgccga
     1441 agccttcacc cgcttcgcgc tctttcgccc agcgccctat cagttcatca agcgacgcca
     1501 ggatttccgg ctcggtgatg ttttctttat acatccgtgg gatacgtgtc ccaatgcggt
     1561 tgccgccaag atgcaggttg tagcgacccg gcgctttacc caccaggccc acttccgcca
     1621 gcatcgcgcg accacaaccg ttcgggcagc ctgttacacg catcacgata tgctcatcgc
     1681 tgacaccatg tttcgccatt aaattatcga tgttgtcgat aaaagacggc aggaaacgct
     1741 ccgcttccgc catcgccagc gggcaagtcg ggaacgagac gcaggccatt gagttttcac
     1801 gctgcggcgt gacggcattc attaacccgc tctctttggc gatcttctcg atcttcgctt
     1861 tctcgctttc cggcacaccg gcgatgatca gattctggtt cgccgtaatg cggaaatcac
     1921 ctttgtggat cttcgcgatc tccagcaggc cggttttcag cggacgcccc ggataatcaa
     1981 ggatgcgacc attttcgata aacagcgtca ggtgccagtt atcatcaatg cccttaaccc
     2041 agccaatacg atctccgcgt ccggtgaact catacggacg gatcggttca aatttgatcc
     2101 ccgcgcgacg ctccacttcc gctttaaacg tctcaacccc cacgcgctcc agcgtgtatt
     2161 tggttttggc atttttacga tcggttcggt taccccagtc acgctgagtt gtcacgacgg
     2221 cttccgccac tgccagcgta tgctccagcg gcagatagcc aaactcactc gccgtgcggg
     2281 cgtaggtttt cttgttgccg tgttcgatag aaagtccacc gcccaccaac aggttaaagc
     2341 ccaccagctt gccgttttcg gcgattgcca cgaagttcat gtcgttggcg tgcagatcga
     2401 tatcgttctg tggcgggatc actaccgtgg ttttgaattt acgcggcagg taggtctggc
     2461 cgaggatcgg ttcttcatcg gtggtggcga ctttttcctg atcgagccag atctccgcat
     2521 acgcgcgggt acgaggcagc agatgttcag agattttctt cgcccactcg tacgcttctg
     2581 cgtgcagctg cgactcgtaa gggttcgagg tgcagagtac gttacggttc atgtcgttag
     2641 cggtcgccag cgcatcaaga ccgaccgagt gcagcatctg gtgcaccggt ttgacgttct
     2701 ttttcagaat gccgtggaac tggaatgtct ggcggttggt caggcgaatg ctgccataga
     2761 tggtgttttc accggcaaat ttatcgatcg cctgccactg tttggtggtg ataactccac
     2821 ccggcagacg gcagcgcaac agcatcgcat ggcgcggctc cagcttctgt tcagcacgtt
     2881 cggcgcggat gtcgcggtca tcctgctgat acatgccgtg gaagcggatc agcaggaagt
     2941 tatcgccctt aaagccgccg gtcaggccgt cgtttaaatc ttccgcaatg gtgccgcgca
     3001 ggtagttgct ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg accactaaag
     3061 gccctggatg tttttcgctc attagtagac atctcgctga taacggcgct ctacgcgcag
     3121 ctcacttaaa aattcatccg ccgcttcggt gtccatgcca ccaaattcgg caatcacttc
     3181 cagaagtgcc tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga cataaatgtg
     3241 ggcaccgtca ttgatccagc gccacagctc cgcgccctgt tcgcgcagtt tgtcttgtac
     3301 gtaaactttt tctttttgat cgcgcgacca ggcaagatcg atacgcgtca gcacgccctc
     3361 tttgacgtaa cgctgccact ccacctggta gaggaaatct tccgtaaagt gcgggttgcc
     3421 aaagaacagc cagtttttac ccggcgcttc gtcagcggcg cgctgctgca taaaggcgcg
     3481 gaacggcgcg atgccggtgc ctgggccaat cataatcacc ggggtttccg ggttagcggg
     3541 caggcggaag ttatcgttat gttcgataaa tacgcgaact tcgccctctt cttccacgcg
     3601 gtccgcgagg aagctggagg caccaccggc acgggcgcgg ccttccacgt cgtaacgcac
     3661 cacaccaacg gtgacgtgta cttcgttctc gacttcctcc tgcgaggagg cgatggaata
     3721 cagacgcggc gtcagcgggc gcagcagatt aatcagcgct tcggcgtcca gttgcgccgg
     3781 agagaagcgc accatgtcga caatcggcgt agtcgcggcg taatgctgta acttcgcttt
     3841 atcgcccacc agcggcaaca gcgtttcgct gcgggtaagc gtggcatagt tctcaacaat
     3901 attggcggtg ttgacggtca gttcgaagtg ccactgtagc gcttcgttca gaggcaacgt
     3961 tttgccctcg acggtgacag gttcatcgcc tttcagccac agcagttcga caagttcttt
     4021 caccagtgcc ggatcgttct gataccagac acccagcgcg tcacccggct ggtaacgcag
     4081 gcccgagtca cctaagtcaa tttcgatatg gcgaacgtct ttttcagagt tacgcccggt
     4141 aattttctgg ttaaccgaaa ggctcgctac cagcggcgcg tctttgctgt acgggctggt
     4201 gtggatttca tttaccacgc cagtagcgac ggattgcgaa ggtgccgcga caggcgcacg
     4261 cgatttaagc gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct ggtattcaac
     4321 gtcggcgtcg acacggtcga gcaggcgttc accgcccagt tccgccagct tgctgtcgaa
     4381 atctttcccg gactggcaga aaaattcata agaactatcg ccgaggctaa acacggcaaa
     4441 agcggtattt tccagcttcg gcgctttttt ggagaacagg aacttatgca gcgcgacggc
     4501 ttcttccggc ggttcccctt ctccttgcgt tgacgtcacc acgataagca gtttttcgct
     4561 ggcgatttgt ttgaatttat agtcgcccgc gttcaccagc ttaacgttca gttttgccgt
     4621 caataaatca tcacgtaatg cttcagcaac ccggcgcgca ttgccggttt gcgaagcaga
     4681 gataatagtt atacccggca tttctgcggc tggcgctggc gtcgctgcaa gcgcagcagg
     4741 ctgctgattg agcacgcccc agaaatagcc agaaacccag gcaagctggg tgggagttaa
     4801 atcggtcgtg gccgcctgaa ggcgtgccag ttgctccggg ttcaacggaa gcaacgcgga
     4861 aggtgggacc tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg tttctgcgcc
     4921 ctgtcagcgc ccataaaaca gaagagaagg taaggttaac ggggtgaacg gtgtggatta
     4981 aagacgggat agcgataact aataaccaaa tcgactaacc tgttttagca atagtcttta
     5041 acaacaaaat agattaacca acctaatgaa aaacaaatga atttagccaa tcattaagat
     5101 aaatcagcga ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaga gcctttccgg
     5161 tactctacgg cggttttatc gtcctgtaga gaaattatga tgtccaccac gttatttaaa
     5221 gatttcacct tcgaagccgc tcaccgctta ccacacgtcc cggaagggca taaatgtggt
     5281 cgcctgcacg ggcattcctt tatggtgcga ctggaaatta ccggggaagt cgatccgcat
     5341 acgggctgga ttatcgattt cgctgaacta aaagcggcgt ttaagccaac ttatgagcgc
     5401 ctcgatcacc attatctcaa tgatattcca ggtctggaaa acccaactag cgaagtttta
     5461 gcaaaatgga tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc ggtgatggta
     5521 aaagaaacct gcaccgctgg ttgtatctat cgcggcgaat gataagagtg tggcggcggt
     5581 caatttccct taagtaacgc tatgttaggg tgttgtgttc aggatatctg gggcatgaca
     5641 tggaagacga ctgcgacatt attattattg gtgccggtat tgcaggcacc gcttgcgcgt
     5701 tacgctgcgc gcgagcgggt ttatccgttt tgttactgga acgcgctgaa atccccggca
     5761 gcaaaaatct ttccggcggg cggttatata cccatgcact cgcggaactc ctccctcaat
     5821 ttcatctgac cgcgcctctt gaacgacgca tcactcacga aagcctttcc ctgttaacgc
     5881 cggattgcgc aacgacgttt tccagcttac agcccggcgg tgaatcctgg agtgtattac
     5941 gtgcacggtt cgatccgtgg ctggttgccg aagccgaaaa agaaggtgtc gaatgcatcc
     6001 ctggtgcgac ggtggatgca ctgtatgaag aaaacggcag ggtgtgtggt gtcatttgtg
     6061 gtgacgatat tctccgcgcc cgttatgtgg tgctggcaga aggtgccaac agcgtcctgg
     6121 ctgaacgtca cgggttagtg actcgtcctg ctggcgaagc gatggcgttg gggatcaaag
     6181 aagtgctgtc gctggaaaca tccgctattg aagaacgttt tcatctggag aataacgaag
     6241 gcgcagcgtt gctgttcagc ggcggaatct gtgatgactt acccggcggc gcatttcttt
     6301 atactaatca acaaacgctc tcgttaggga ttgtttgccc actctcttcc cttacgcaaa
     6361 gtcgtgttcc ggcaagcgag ctgcttgctc gctttaaaac gcatccggca gtgcgcccgc
     6421 ttatcaaaaa cacggaatca ctggagtatg gtgcgcatct ggtgccagaa ggtggcttgc
     6481 acagtatgcc ggtacaatac gccggtaacg gctggctgct ggtgggcgat gcgttgcgca
     6541 gttgcgtcaa taccggaatt tctgtgcgcg gcatggatac ggcgctaact ggcgcgcagg
     6601 cggcggcaca aacactgata agcgcctgcc agcaccgcga gccgcaaaat ctgtttccgc
     6661 tttatcatca caacgtagag cgcagcctgc tgtgggatgt tctacagcgt tatcagcatg
     6721 ttccggtgct tttgcaacgc ccgggatggt accgtacgtg gcctgcgtta atgcaggata
     6781 tttcccgcga tttatgggat cagggtgata aacctgttcc accgctgcgc cagttactct
     6841 ggcgtcattt acgtcgtcat ggcttgtgga atctggcggg cgatgttatc aggagtgttc
     6901 gatgtctgta gcccgtaatc tctggcgcgc tgctgatgcg ccgcacattg ttccggctga
     6961 ctccgttgag cgtcagatgg tacaacggtt gattaacgcc tgtccggtag gtcttttttc
     7021 gctcacaccg gaaggtgact tacgcgttga ttatcacggc tgcctggagt gtggcacctg
     7081 ccgtttgctg tgcgacgaat ccacactaca acagtggcgc tatccgcctt ccggattcgg
     7141 catcacctac cgatttggat aaataataag gataatttat gcccctctta cacctgctcc
     7201 gccagaatcc ggtgattgct gccgttaaag ataatgccag cctgcaactg gccatcgatt
     7261 ctgaatgcca gtttatttcc gtcttgtacg gcaatatctg caccattagt caaatcgtta
     7321 aaaagattaa gaacgctggg aaatatgctt ttattcatgt tgatttgctg gaaggcgcgt
     7381 caaataaaga agtggtgatt cagtttttga aactggtgac cgaggcggat ggaattatca
     7441 gcaccaaagc atcgatgcta aaagcggcaa gagcggaagg ttttttctgt attcatcgcc
     7501 tgtttattgt tgattcgatt tcgtttcaca acattgataa gcaagttgcg caatcgaatc
     7561 cggattgtat tgagatcctg ccaggctgca tgcccaaagt gctgggctgg gtgacggaaa
     7621 aaatccgcca accgctgatt gctggcgggc tggtatgcga tgaagaagat gcgcgtaatg
     7681 cgattaacgc gggtgtcgtg gcgctttcca ccacgaatac cggagtctgg acgttagcga
     7741 aaaaattgct ttgacgggat aatcgtaacc aattgaattt ggtttgattt gtaggccgca
     7801 cgccacatcc gacattcagc gcctgatgcg acgcttaaca cgtcttatca ggcctacagg
     7861 tccccagtcc agaaccgtag gcaggatcat ccggcagcac gttattggca ttctgcgtca
     7921 atgtttgtca ccagtgcctc cagcatcact ttccagtcat caaccacgcc aacatctgcc
     7981 tgtgaaaata ccgctgcgct ggcgtcatga ttaatcgcca ccacaaactt gctattgcgt
     8041 acgcccgcca ttaacgctgc tgcgccagaa gcgccaacaa caatacacac ctcaggtgcc
     8101 agcagatgtc cggaaatacc aatcactttt tcagcatcaa cgccaccgtt catcactcgc
     8161 gcccgactgt agcccacttc ggctcccagc ttttatgcca gcatagcgat ttcctggtta
     8221 tctgcctctc cgccctgccc caccaccaac acccgtctgg cctcagctaa tgggtcgcgg
     8281 gtaacatttt tcaggttctc tatgctaacc agccagtccg gaagcgcacc tgggactatg
     8341 ttcagttgct gcataccaga gggcaacgtg gcatttttat ctgctcccgc ctggcgcgcc
     8401 agggaaagac atagcgggcg tttttctgtt tgcagcgtcg ccgttagcgc atttccccag
     8461 tgggatttgc gcacgcttac gattggaatc tctaacgaag tcacctggca gatgcttgca
     8521 ccatgtaaac gccaggccag ccgagtcgac agttcatcgc caaacgttcc cggcggaaag
     8581 agaacaacgt tcgcaggcgt tcgctgccac tgttccacca gcgcatccag cacctgttcc
     8641 accactacgg gctgtggttc tatttgccag tgcgccaggg tacaaccgct gaaatcctgc
     8701 gccgccagcc agcccgctaa tgcggcattc tcctgattta ttgtcacaat tgcgatattc
     8761 atggctgcat cctttggcgt aaatagtcct gccatagctt ttgcgctttt tctgctaccg
     8821 tttgcccatc aatcagcgtc gctccgcgtc gttgctctgc tcttgccagt tgcagacact
     8881 gcatcgctgg caattcggca gcaacagttt tacggataat ttccgctttc cccgcagcca
     8941 ttcgctggcg cattcccggt actggcaagg ccacttcacc gcactgacgc acggcaatca
     9001 ccgcaggcaa gcgaatccga caacagcgca gcccatgttc agtacgctgt tcgagggtga
     9061 taaacggcgc gtcgagagtg aaacgctcca cctgggtgaa gcaaggccag cccagcattt
     9121 ccgccagcaa aaatggcgtt tgcccattct gcccttcgct gctttggcag ccggtaatta
     9181 tcagatccag cggattctga cgctgccatt cggcaatatg gcgagcgaca aattccgacg
     9241 caaagcgcag atctgccgcc gtctccagca atacagcttc ctcaaaccca agagccatga
     9301 gatagcgcag ccagtgcaac gcccgttcat cccccatgct caacgcggtt aaagacatcg
     9361 gcgtaccgtt ttttctctgc gccaacagca gcgcggcggc agcctgttca tcagcaccga
     9421 gtaaacttcg cagtagcgaa atatccggtc cgctattacc atgagccgcc gcctgccact
     9481 ctttttccgc cagcattccg gcatccggtt cggctttaaa cgctaacaga atgttcattc
     9541 gcacctccta catttcccgc cgccaccagc gggagtgctt tggtttctgg tgcccatagc
     9601 caggttacaa caaaaccagc caacaacact gccgccagga gcaataaggt gacttgcatt
     9661 ccccactgcg ccagcaccca cggcagcagg ccagtactta ccgccgcccc caaccgactc
     9721 atggcggtgg caaaaccgac gcccagcgag cgaatgtcag taggaaaact ttccgcaggc
     9781 aaaatgccca ccagattact gactgccgaa atggtggtgc tgaagagaac aaaagcagca
     9841 gcgttaatga actgccggaa ggcaaacagg ccatcactac cagcgttgcc gccagcagca
     9901 aaaaacttcc cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact aatcccagca
     9961 gcgcgcccac aattaacaac gcattaagca tcaggctggc agtcagcgca tcttccagtc
    10021 caatagtctg ggcgatagtt ggcagccagg tataaatcac aaaccatggg attacgaggc
    10081 agacaaagaa gacgctgtta aacgccgtgc gccgccagta gcgcgaagag aacaaggttt
    10141 tgatgtgttt atgggtcgcc gttgccactt catcgcccag taaaacatgg ggaccaaaat
    10201 agcgatgcac gatagcgtga gcttctgcaa aacgcccctg gcgcagtagc cagcgtggcg
    10261 attctggcgt tccccagcgt aataacgtaa tcaacaacgc gggcagagct gccgatgcca
    10321 gcagccagcg ccaggcctcc gggttttcgg aaataaagtg atgtccggca atacttgcca
    10381 acacatagcc tacggtccac accacgctga atgcgcccag caaaacaccg cgatggcggc
    10441 gcggggaaaa ttcagccagc aaggtgtgac ctactgagta atcgcctccc agaccaatgc
    10501 cgatcaaaat gcgcagtcca ataagatgct ctggcgtggt ggcaaaaaat tgcaagaacg
    10561 aagcgagcgt aatcagcaaa aagctgaagg tgaagatttt ttgccgacca atatggtcgg
    10621 agatccaccc aagaaccagg ctgccaagaa acaaaccaag gtgcgccgag ccgccgatca
    10681 ttcccgccat aaacggcgtc agttgcatgg cgggcgtaag ttgaataatg gcgtaaccaa
    10741 tgacgccgag aacataaccg tcggtcaggt gtgcgccgaa agtgagcgca gcaattcgac
    10801 agtgaaaacg gttaagcggt aaatcatcca ttcgcaccgg tgaagtgttc atctttaccc
    10861 cttcattgtc cggtaataag aacggcttct cgcctgagaa gccgaaaagg caacgcccca
    10921 tcatcgcgga acgctgcctg acacattatt tttcaatcgg atagatagta cccgtattca
    10981 taatgccatt aggatcgaac tgctttttca gcccttccag caacgcccac gcgctgccgt
    11041 gttccagctt gctccagtga acgcgatgtt taccgatacc gtggtggtgc accatcgaac
    11101 caccgaggcg aatggtttct tcgcaaatga ttttgttgag cggattgtgg tacttgtcga
    11161 tttcctcttc cggcttacag ttgacaacgt tgtagtcgta gacgaagtac atgttggtgc
    11221 cgttctgata gctatgagag gagtgaccgc ccagcatggt gatgtcgtcg gcgtgcggga
    11281 actcggtacg aatacggtta ataacgcttt cgtagatttc gtggatgcag ctccagcagc
    11341 cggacacttc ggtagtaaag cccatgttgc cggttttgag gatctgcaca cgttcggcag
    11401 ccactttatc cggtccccag ttcaggttgt taaaccaggt ttcgatcagc ttgctgtcca
    11461 cgcgttggca ttgcgggtag cgggcaacga tttccgcaat cccttcgccc gtcgccttcg
    11521 caatgcgagg gttaccttca gccataaaga tcagcacgca ttttccgtcg gcaaaatggg
    11581 tgaagtgttg ggtgccatct tcagcgtcat acaaacgagc gatcgacgga cgataccctt
    11641 ccaccatgat ttcacgcagg atgttgaagc cggttttcat gtcttccagg atatagccgt
    11701 agaagaggtt gttttccggg gtgaatttaa agattttcac tgttacttca gtgatatagc
    11761 acaatgcacc ttcgttgccg atgatgatgt gacgaatgtc cgggccagcc gcgcggcgtg
    11821 gcacgttttt aatgcgtgtg acggtgccat ctgccaacac tgcctccaga ccaacgacca
    11881 tatcttcgat tgcgccgtag agtgtggaga actgcccgat actgcgggtt gctaccaggc
    11941 cgcccatctg cgccagcggc tttgactgcg gagaatgccc cgtggtgtaa cctttttcac
    12001 gcaacgcgtt ttccagcacc tccagcggaa caccacattg cgccgtcgcc tgcatattct
    12061 caatatcaat attaatgatt tgattcatgg cggaaccgtc gagcaccacc gagttttcta
    12121 caacagtttc cagcccacct tcggtggcgg aagcaccggt acgcggcaca ccgttaattt
    12181 tgtgcgcatt cataaaattc agcacacggg atacttgctc tgtggaaccg agttttacga
    12241 ccgctgccgg aatcggcaaa gtataaatgc catgaatatc cggaaattta cgaaaacggt
    12301 caatactgtt cttctttaat actgtttcat cggtaattac gcgatctgca ccaacaattt
    12361 cctttagctg gtcgacaatc gctgcgcgag ataaagacat agtaattcct tcctgataat
    12421 aaaaatggaa taatcattaa taagagaaaa cagaaaccac cgttatgggt tagcgcacta
    12481 aataaccgcc atcgaccact aataaatgcc cgttgacata attcgatgcc tgacttgcga
    12541 gaaacacggc tgcgcccatt aaatcctgag tatcgcccca acggtttgcc ggaatatgat
    12601 caagaacgcg ctgattggtt tctggattac tgcgtgtcgc cagcgtaatg tctgttgcat
    12661 aatagccagg ggcgatacca tttacctgaa tattatattg acctagttca tcacaataag
    12721 ctttggtgaa tccggcaaga gcatgtttag tagcagaata tgcaggtgac cattgtccac
    12781 ctaagtaaga gaacaatgaa cagatattaa tgattttacc gcttttctgc gggatcataa
    12841 tttttgccgc ttcgtagctt aactcgaatg cggcggtcag gttcacatca atcatcgggt
    12901 cccagtcggc acgaccgaag tccagcacct tattcagctt acaaataccg gcattgttaa
    12961 ccagaatatc aactgtaccg aaacgctcac agcaggcagc gataatcttc tgcggcgcgc
    13021 cttctgcggt gatatccacc tgcatgaagt ccacctcaac accctgattt tcaatcattt
    13081 cctttgtttc gccgttatct ttgacaaaac ttggaataaa gacatttgcg cctgctttgg
    13141 ccaacgccat ggcaaatgcc tggcctaaac cgctattccc gccggtaaca attgcggttt
    13201 tacctttcag ggagaaaaaa tccattgaga acgcattgag agattcgatt gacatagtta
    13261 gctccaattt ccgtaaggca aaaaaaaaga aaggtaactt cccccgcgag agggaagtta
    13321 cctttctcat tatctctggt aactggagat aatttttagc ataaggacat aactgctaat
    13381 gtgatcgtaa tcacagtgtg atattcgtat tatca
//
LOCUS       DAZFDC010000127        12989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFDC010000127 DAZFDC010000000
VERSION     DAZFDC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.127"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            322..1035
                     /gene="aphA"
                     /locus_tag="ACHJ48_003837"
     CDS             322..1035
                     /gene="aphA"
                     /locus_tag="ACHJ48_003837"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO4709303.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1146..1562
                     /locus_tag="ACHJ48_003838"
     CDS             1146..1562
                     /locus_tag="ACHJ48_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO4709304.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1566..1922
                     /locus_tag="ACHJ48_003839"
     CDS             1566..1922
                     /locus_tag="ACHJ48_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO4709305.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1957..4779)
                     /gene="uvrA"
                     /locus_tag="ACHJ48_003840"
     CDS             complement(1957..4779)
                     /gene="uvrA"
                     /locus_tag="ACHJ48_003840"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO4709306.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5034..5570
                     /gene="ssb1"
                     /locus_tag="ACHJ48_003841"
     CDS             5034..5570
                     /gene="ssb1"
                     /locus_tag="ACHJ48_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO4709307.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5669..5950)
                     /locus_tag="ACHJ48_003842"
     CDS             complement(5669..5950)
                     /locus_tag="ACHJ48_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO4709308.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6379..7965
                     /gene="pdeC"
                     /locus_tag="ACHJ48_003843"
     CDS             6379..7965
                     /gene="pdeC"
                     /locus_tag="ACHJ48_003843"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO4709309.1"
                     /translation="MNHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIVPVNGYTIVSADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVPRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(7968..8291)
                     /gene="soxS"
                     /locus_tag="ACHJ48_003844"
     CDS             complement(7968..8291)
                     /gene="soxS"
                     /locus_tag="ACHJ48_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO4709310.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8377..8841
                     /gene="soxR"
                     /locus_tag="ACHJ48_003845"
     CDS             8377..8841
                     /gene="soxR"
                     /locus_tag="ACHJ48_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO4709311.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9387..10736
                     /gene="ghxP"
                     /locus_tag="ACHJ48_003846"
                     /gene_synonym="yjcD"
     CDS             9387..10736
                     /gene="ghxP"
                     /locus_tag="ACHJ48_003846"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO4709312.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10887..12536
                     /locus_tag="ACHJ48_003847"
     CDS             10887..12536
                     /locus_tag="ACHJ48_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO4709313.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12690..>12989)
                     /locus_tag="ACHJ48_003848"
     CDS             complement(12690..>12989)
                     /locus_tag="ACHJ48_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO4709314.1"
                     /translation="VNFNNANLNNCHFNCSVLTKAWMFNTRLYRVNFDEASVQGMGIS
                     ILRGEENIPINSDTLVTLQKFFEEDCTSHTGMSQTENNTHEVAMKITADIMQHAD"
BASE COUNT         3021 a         3199 c         3405 g         3364 t
ORIGIN      
        1 aacttcgatt gtttttgtaa tgctgtatca ttaagttcat taaatcgtac agcagataaa
       61 tgttctatca aatttcgctc atttgcagag tattcatcat aataaacgta aaattaatgt
      121 atccttacat cgagcattaa ccatttttta caaaaaaacg acaggaacgt atttactagg
      181 ttaaatataa tcatcctgct tttcatcaca aaaaccgcag ataatccttc ctttcccctg
      241 tagctggcgt tatggtcaga tagtttttgc aacaaatctc acaataaaaa gtttcaacat
      301 actgactatt tagggaaaaa tatgcgcaag atcacacagg caatcagtgc cgtttgctta
      361 ttgttcgctc taaacagttc cgctgttgcc ctggcctcat ctccttcacc gcttaaccct
      421 gggactaacg ttgccaggct tgctgaacag gcaccaattc attgggtttc ggtcgcacaa
      481 attgaaaata gcctcgcagg gcgtccgcca atggcggtgg ggtttgatat cgatgacacg
      541 gtactttttt ccagtccggg cttctggcgc ggcaaaaaaa acttctcgcc agaaagcgaa
      601 gattatctga aaaaccctgt attctgggaa aaaatgaaca acggctggga tgaattcagc
      661 attccaaaag aggtcgctcg ccagctgatt gatatgcatg tacgccgtgg tgacgcgatc
      721 ttctttgtga ctggtcgtag cccgacgaaa acagaaacgg tttcaaaaac gctggcggat
      781 aattttcata ttcctgccac cagcatgaat ccggtgatct ttgcgggcga taaaccaggg
      841 caaaatacaa aatcgcaatg gctgcaggat aaaaatatcc gaatttttta tggcgattcc
      901 gataacgata ttaccgccgc acgcgatgtc ggcgctcgtg gtatccgcat tctgcgtgcc
      961 tccaactcta cctacaaacc cttgccacaa gcgggtgcgt ttggtgaaga ggtgatcgtc
     1021 aattcagaat actgacagag cgggagagcg tgacactctc ccgaatgctg tttttttaat
     1081 cacaccttta tcctttcgct gtcttgctgc aaactgatta agagagtttt atcaaggagc
     1141 agcacatgtg gtatcaaaag acgctcacgc ttagcgccaa atctcgtggg tttcatctgg
     1201 taacggatga aattctgaat cagctggctg atatgccgcg cgttaacatc ggcttactgc
     1261 atctgttgct gcaacatacc tccgcctctc tgacacttaa tgagaactgc gatcccaccg
     1321 tacgccacga catggagcgt tttttcctcc gcaccgttcc cgacaacgga aattatgagc
     1381 atgactatga gggagcagac gatatgcctt ctcatatcaa atcctcaatg ctgggaacat
     1441 cgcttgtatt gccggtgcat aaagggcgta ttcagaccgg cacctggcaa ggcatttggc
     1501 tgggggaaca tcgcatccac ggcggatcgc gtcgcatcat cgcgacacta caaggggagt
     1561 aaaaaatgac catttcggag ttgctacaat attgcatggc aaaaccaggc gcagaacaga
     1621 gcgtgcataa cgactggaaa gcgacgcaga tcaaagtgga agatgtactg tttgcgatgg
     1681 tgaaagaagt agaaaatcgc ccagctgttt cgctgaaaac cagcccggag ctggcggagc
     1741 tgctacgtca gcagcacagc gatgtgcgtc caagccgcca tctgaataaa gcgcactgga
     1801 gcaccgtgta tctcgacggt tcgctgccgg attcgcaaat ctattatctg gtggatgcgt
     1861 cttatcagca ggcggtgaat ttactgccgg aagaaaaacg taaattgctg gtgcaactct
     1921 gaaaggaaaa ggccgctcgg aaggcggcct taacgattac agcatcggct tgaggaagcg
     1981 tgccgtatgc gaagcttcgc actccgcgac ggtttctggc gtaccggaga cgaggatctc
     2041 gccgccgcca ctgccgcctt ccggccccag gtcgacaatc cagtcagcgg ttttaatcac
     2101 gtcgagattg tgctcaatca ccacaatggt gttgccctga tcgcgcagtt tatgcagtac
     2161 gtcgagcagt tgctgaatat cggcgaagtg cagaccggtg gtcggctcgt cgagaatata
     2221 cagcgtctgc ccggtgccgc gttttgacag ctcacgcgcc agcttcacgc gctgggcttc
     2281 accaccagaa agtgtggttg cggactgccc caagcgaatg tacgtcagac caacgtccat
     2341 caacgtttgc agcttacgcg ccagagctgg caccgcatca aagaactcac gcgcctcttc
     2401 gatggtcata tccagcactt cgtggatggt tttgcctttg tacttaatct ccagcgtttc
     2461 acggttatag cgtttacctt tgcactggtc gcacggcacg taaatgtccg gcaggaagtg
     2521 catctccact ttgatcacgc catcgccctg acaggcctcg cagcgtccgc cacggacgtt
     2581 aaagctgaaa cgtcctggcg tataaccacg cgcacgggat tccggtacgc ccgcaaacag
     2641 ttcgcgcaca ggcgtaaaca cgccggtata ggtcgccggg ttagaacgcg gagtacgacc
     2701 aattgggctt tggtcgatat cgattacttt gtcgaaatgc tccagtccct gaatatcgcg
     2761 atacggtgcc ggttcggcga tggtcgcacc attcaactgg cgttgggcaa tcgggaacag
     2821 tgtgtcgtta atcagcgtcg atttaccgga acctgaaacc cctgtgatgc aggtaaacag
     2881 accgactggc agcgttagcg tcacgtcttt caggttgttg ccgcgtgcgc cagtcagctt
     2941 aagtactttt tccggattcg ccggaacgcg tttcttcggc acttcaatct tgcgtttacc
     3001 gctcatgtac tgtccggtca acgactccgg caccgccata atcgcttcca gcggaccttc
     3061 cgcgaccact tcaccgccat gtacacctgc accaggaccg atatcgatca catgatcagc
     3121 ggcgcgaatc gcgtcttcgt cgtgctccac cacaatcacg gtattaccga gatcgcgcag
     3181 atggataagc gtacccaaca ggcgctcgtt atcgcgctgg tgcaggccga tagacggctc
     3241 atccagcacg tacataacgc caaccaggcc cgcaccaatc tggctcgcca gacggatacg
     3301 ctgggcttca ccgccggaaa gcgtttctgc cgagcgggaa agcgtcaggt aattcaggcc
     3361 gacgttaacg aggaatttca aacgatcgcc gatctcttta agaatttttt ccgcaatctt
     3421 cgcccgctga cctgcgagtt tgagattgtt gaagaattcc atcgcatgac caatgctcat
     3481 gtcggagatg gcgggcagcg gcgtattctc gacatacacg tggcgcgctt cccgacgcag
     3541 acgcgtccct tcgcagctgg cgcacgggcg attgctgatg aacttggcta attcttcacg
     3601 taccgcactg gattctgtct ctttataacg gcgctccata ttgtgcagca cgccttcgaa
     3661 cggatgacga cggatggagg tatcgccacg atcgttcatg tatttgaatt caatgttttc
     3721 tttgccagaa ccgtacaaca ccactttatg cacgttcgcg ctcaggctgc cccacggcgc
     3781 ttcgacgtcg aacttatagt gatctgccag cgatttcagc atctggaaat aatagaagtt
     3841 gcggcgatcc cagccacgga tcgcaccacc tgccagcgac agttccggat tctggatcac
     3901 gcggtcagga tcgaaatatt gctgtacgcc aaggccgtcg caggtcgggc aggcacctgc
     3961 cgggttgtta aacgaaaaca gtcgcggctc cagttcacgc atactgtagc cgcaaattgg
     4021 gcaggcgaag ttggcggaaa acagcagctc ttccgctttc gggtcgtcca tatccgccac
     4081 taccgcggta ccaccggaaa gctccagcgc ggtttcaaac gactcggcaa gacgttgggt
     4141 aagatcgtca cgcaccttga agcgatcaac caccacttca atagtgtgtt tcttttgcag
     4201 ttccagtttc ggcggatcgg aaagatcgca gacttcgcca tcaatacgag cacggatgta
     4261 accctggctt gccaggttct ccagcgtttt ggtgtgttcg cctttgcgct ctttaatgat
     4321 tggcgcgagc agcatcagac gcttgccttc cggctgcgac agcacgttat ccaccatctg
     4381 gctgacggtt tgcgccgcca gcgggacgtc gtggtccgga cagcgcggtt cgccgacgcg
     4441 ggcgtacaac aaacgcaaat agtcgtggat ttcggtgatt gtccccaccg tagaacgcgg
     4501 gttatgagac gtcgatttct gctcaattga gatggcagga gaaagcccct caatatgatc
     4561 gacgtccggc ttttccatca gtgacagaaa ctgccgcgcg taggcggaaa gggattcaac
     4621 gtaacggcgc tgcccttcgg catataaggt gtcgaaagcg agcgaggatt tgccagaacc
     4681 cgaaagcccg gtcacgacaa tgagcttgtc gcgggggata acgaggttga tgtttttgag
     4741 attatgggtg cgggcgcccc gaacttcgat cttatccatt cacctttccc ggattaaacg
     4801 cttttttgcc cggtggcatg gtgctaccgg cgatcacaaa cggttaatta tgacacaaat
     4861 tgacctgaat gaatatacag tattggaatg cattacccgg agtgttgtgt aacaatgtct
     4921 ggccaggttt gtttcccgga accgaggtca caacatagta aaagcgctat tggtaatggt
     4981 acaatcgcgc gtttacactt attcagaacg attttttttc aggagacacg aacatggcca
     5041 gcagaggcgt aaacaaggtt attctcgttg gtaatctggg tcaggacccg gaagtacgct
     5101 acatgccaaa tggtggcgca gttgccaaca ttacgctggc tacttccgaa tcctggcgtg
     5161 ataaagcgac cggcgagatg aaagagcaga ctgaatggca ccgcgttgtg ctgttcggca
     5221 aactggcaga agtggccagc gaatatctgc gtaaaggttc tcaggtttat atcgaaggtc
     5281 agctgcgtac ccgtaaatgg accgatcaat ccggtcagga tcgctacacc acagaagtcg
     5341 tggtgaacgt tggcggcacc atgcagatgc tgggtggtcg tcagggtggt ggcgctcctg
     5401 caggtggcaa tatcggtggt ggtcagccgc agggcggttg gggtcagcct cagcagccgc
     5461 agggtggcaa tcagttcagc ggcggcgcgc agtctcgccc gcagcagtcc gctccggcag
     5521 cgccgtctaa cgagccgccg atggactttg atgacgatat tccgttctga tttgtcatta
     5581 aaacaatagg ttatattgtt ttaaggtgga tgattaaagc atctgccagc cataaaaaag
     5641 aagcctccgt tatggaggct tctacgtatc aggtcaaaat cattggccac tgtggcggtg
     5701 tctgattcat ctcttctatc atcaccgact cgatgttgtg ccagatggcg gtgatgtccg
     5761 ccattgtgat gccaagcaac cccattgcga accagcaggc ggaaaccacg cccagaccgc
     5821 tgctgatcac tgcaagcccg acataatcag accgacgaaa acggatgttg agcgtgctgg
     5881 ccagaaacat cattacggca ctaagaagtt gccagcgaag aagaaccacg ccagtggtga
     5941 gggtagccat caaacaattc ctctgaaaag agccgatgcc ctggacagcg cggcgttgtt
     6001 cacgggaggc gtggtacact ctggctatcg cggggcttgc agaacacaaa aatgaaacac
     6061 tctgtttgtt tcattaattt tgtgaactat atcacaattg attgtttgtt agccagatta
     6121 ggtcgtgact tttattgctg tacagattat gtggtttttc agtggcatta agggtatatc
     6181 ttcccgccgc ctctgtattc ctgtagaaaa ttaattttga atatcaatga attattttca
     6241 tccaagtgac gattaaaaag gtatcaattt caaatcaggc aaaagtgcta tttataccgt
     6301 aagatttatc taaagacgtc ggtagccagg gtttcacctt gcaatggccg ggtataaaca
     6361 ggcaggaaat tgatagcaat gaatcatcgt gcacggcacc aattactggc gttgccgggc
     6421 attatctttt tagttctctt tcccatcatt ctttcgctat ggattgcctt cctttgggca
     6481 aaatcagaag tgaataatca gctccgaacc tttgctcaac tggcgttgga taaatccgag
     6541 ctggtcattc gccaggcaga tttagtgagc gatgcagctg aacgctatca ggggcaagtt
     6601 tgcactccag cccatcaaaa gcgaatgttg aatattattc gtggctatct ttatattaat
     6661 gaattgatct atgcccgtga taatcatttt ttatgctcat cgctgatagt gcctgtaaac
     6721 ggctatacga ttgtatcggc cgattataag cgtgaaccta atgtttctat ctattattac
     6781 cgcgatacgc cttttttctc tggctataaa atgacctata tgcagcgggg aaattatgtg
     6841 gcggttatca accctctctt ctggagtgaa gtgatgtctg atgacccgac attgcaatgg
     6901 ggtgtgtatg atacggtgac gaaaaccttt ttctcgttaa gcaaagaggc ctcagcagca
     6961 acgttttctc cgctgattca tttgaaggat ttaaccgtac caagaaatgg ctatttatat
     7021 gcgacagttt attcgacaaa acgcccaatt gcagccattg ttgcgacttc atatcaacgt
     7081 cttatagccc atttttataa tcatcttatt tttgcgttgc ccgccggtat tttggggagt
     7141 cttgttctgc tattactctg gctacgtatt cgacaaaact atttgtctcc caaacgcaaa
     7201 ttgcaacgcg ccctcgaaaa acatcagcta tgcctttatt accagccaat aattgatatc
     7261 aaaacagaaa aatgtatcgg cgctgaagcg ttgttacgtt ggcctggtga gcaggggcaa
     7321 gtaatgaatc cggcagagtt tattcctctg gcagaaaagg aggggatgat agaacaggta
     7381 actgattatg ttattgataa tgtcttccgc gatctgggcg cgtaccttgc aacacatgct
     7441 gatcgctatg tttctattaa tctgtcggcc tccgattttc atacgtcacg gttgatagcg
     7501 cgaatcaatc agaaaacaga gcaatacgcg gtgcgtccgc agcaaattaa atttgaagtg
     7561 actgagcatg catttcttga tgtcgacaaa atgacgccga ttattctggc tttccgccag
     7621 gctggttacg aagtggcaat tgatgatttt ggtattggct actctaactt gcataacctt
     7681 aaatcattga atgtcgatat tttgaaaatc gacaaatcgt ttgttgagac gctgaccacc
     7741 cataaaacca gtcatttgat tgctgaacat atcatcgagc tggcgcacag cctggggtta
     7801 aaaacgatcg ctgaaggcgt cgaaactgag gagcaggtta actggctgcg caaacgcggc
     7861 gtgcgctatt gccagggatg gttctttgcg aaggcgatgc cgccgcaggt gtttatgcaa
     7921 tggatggagc aattacccgc gcgggagtta acgcgcgggc aataaaacta caggcggtgg
     7981 cgataatcgc tgggagtgcg atcaaactgc cgacggaaaa cgcgggagaa ggtctgctga
     8041 gagacataac ccaggtccat tgcgatatca aaaatcggac gctcggtggt gcgcaactca
     8101 acggcggcca gtaacagacg gcgttgacga atgtaatcgc caagcgtctg atgcgtcacc
     8161 gtgcggaaca tccgttgcag gtaccacttt gaatagcctg atttttttgc gacgacatca
     8221 atgttaagcg gctggtcaat atgctcgtca atccatgcga taagatcctg aataattttc
     8281 tgatgggaca taaatctgcc tcttttcagt gttcagttcg ttaattcatc tgttggggag
     8341 tataattcct caagttaact tgaggtaaag cgatttatgg aaaagaaatt accccgcatt
     8401 aaagcgctgc taacccccgg cgaagtggcg aaacgcagcg gtgtggcggt atcggcgctg
     8461 catttctatg aaagcaaagg gttgattacc agtatccgta acagcggcaa tcagcggcgt
     8521 tataaacgtg atgtgttgcg atatgtcgca attatcaaaa ttgctcagcg tattggcatt
     8581 cctctcgcga ccattggtga agcgtttggc gtgttgcccg aagggcatac gttaagtgcg
     8641 aaagagtgga aacagctttc gtcccaatgg cgagaagagt tggatcggcg cattcatacc
     8701 ttagtggcgc tgcgtgacga actggacgga tgtattggtt gtggctgcct ttcgcgcagt
     8761 gattgcccgt tgcgtaaccc gggcgaccgc ttaggagaag aaggtaccgg cgcacgcttg
     8821 ctggaagatg aacaaaacta aagcgccaca agggcgcttt agtttgtttt ccggtctttg
     8881 tctttctctc tatcccgctg gtacacagga gggtttcccc cgacgtcaac acacctcatt
     8941 cgagcacgtg gtggaggttc cggttggtgt tgatgcttta attgtatgtc accgacgttt
     9001 cttcgccagt gtaaaagtat actttttagc cgcaatattt ttgtcatctc agacgatttt
     9061 ttatcgcaat cctcaacggt atacggctcg ataacgctgc aatcttgcgc atcgacgata
     9121 acgtttgcgc atcaattgcc tggtttttca tcgtcaagac aataaaagag aaaaaagcag
     9181 caaacttcgg ttgaaaaagc cgctatgatc gccggataat cgtttgcttt ttttaccacc
     9241 cgttttgtat gcgcggagct aaacgtttgc ttttttgcga cgcagcaaat tgtcgcaaac
     9301 ctggagcagg aagataacgt ttcgctggca ggggattgtc cgccacgcat cttgacgaaa
     9361 attaaactct caggggatgt tctcttatgt ctacgccatc agcgcgtacc ggcggttcac
     9421 tcgacgcctg gtttaaaatt tcacaacgtg gaagcactgt ccgtcaggaa gtggttgccg
     9481 ggttaacaac gtttctggcg atggtctact cggttatcgt cgttccaggc atgttgggta
     9541 aagcgggctt cccgcctgcg gcagttttcg ttgcgacctg tctggttgcc ggactcggtt
     9601 ctatcgtgat gggtctgtgg gctaatctgc cgttggcgat tggttgcgcc atctccctga
     9661 cggcgtttac cgcattcagc ctggtgctgg ggcaacatat tagcgtacct gtcgcgctgg
     9721 gtgccgtgtt cctgatgggt gtgctgttta cggtaatttc tgccacgggt atccgtagct
     9781 ggattttgcg caacttgccg cacggtgtgg cgcacggcac ggggatcggt atcggtctgt
     9841 tcctgctgct cattgccgct aacggtgtcg gtctggtgat taaaaacccg cttgatggtc
     9901 tgcccgttgc gctgggtgat ttcgcgacct tcccggtgat tatgtcactg gtaggtctgg
     9961 cggtgatcat cggcctggaa aaactgaaag tccctggtgg cattctgctg accattatcg
    10021 gtatctcaat tgtcggtttg atcttcgatc ctaacgtcca tttctccggc gttttcgcca
    10081 tgccttcatt gagcgatgaa aacggcaatt cactgattgg cagcctggac attatgggcg
    10141 cgctgaatcc tgtagtcctg ccaagcgttc tggcgctggt gatgacggca gtatttgatg
    10201 ccaccggaac tatccgcgct gtcgccggcc aggcgaacct gctggataaa gatgggcaga
    10261 tcatcgacgg cgggaaagca ctgaccactg actccatgag cagcgttttc tctggcctgg
    10321 tgggtgcagc tccggcagcg gtatacatcg agtctgcggc gggtacggcg gcgggcggta
    10381 aaaccggttt gacggctatc accgttggcg tgctgttcct gctgattctg ttcctctctc
    10441 cgctttctta cctcgttccg gggtatgcaa cggctccggc gctgatgtac gttggcctgc
    10501 tgatgctgag caacgtggcg aaaatcgact ttgctgattt tgttgatgcg atggcgggtc
    10561 tggttacggc ggtattcatc gtgctgacct gtaacatcgt aacaggcatc atgatcggct
    10621 tcgcgactct ggtgattggt cgtctggttt ccggtgaatg gcgcaagttg aacatcggta
    10681 cggtcgttat cgccgtggcg ctggtgacct tctatgcggg tggctgggct atctaatctt
    10741 tcatcggatt ctgaaaacgg gtggcaatgg ctgcccgttt ttattttctc cgcacattgt
    10801 gtgagctttt gcgatattct gaaaaaatga gaattcaggc ataacgtctt tctcagatca
    10861 taaaaaacat cgcaaacagg gaacgcatgg aaatcttctt caccatactg ataatgaccc
    10921 tcgtggtctc gctgtccggg gtggtcactc gtgtcatgcc ctttcagatc ccgcttccgc
    10981 ttatgcaaat cgccatcggt gcgctactgg cgtggccgac gtttggtttg catgtggagt
    11041 ttgatcctga actcttttta gtcttgttta tcccgccgtt gctgttcgct gatggctgga
    11101 aaacgccgac ccgtgaattt ctcgaacatg gtcgagagat tttcggcctc gcgctggcgc
    11161 tggtggtggt caccgtggtc ggcattggct tccttattta ctgggtggtg ccgggcattc
    11221 cgctgatccc cgcctttgcg ctggcggcgg tgctttctcc gaccgatgct gtggcgctct
    11281 ccgggattgt tggcgaaggg cgcatcccga aaaaaatcat gggcattttg cagggcgaag
    11341 cgttgatgaa tgacgcctcc ggcctggtgt cgttgaagtt tgccgtggca gtggcgatgg
    11401 ggacgatgat cttcaccgtc ggcggtgcga cggtcgaatt tatgaaagta gccattggcg
    11461 gtattctcgc cggttttgtg gtgagctggc tgtacggtcg ctcgctgcga ttcctcagcc
    11521 gctggggcgg tgatgaaccc gcgacgcaga tcgtcctgct gttcctgctg ccattcgctt
    11581 cttatctgat tgccgaacat attggcgttt ccggcatcct cgctgcggtt gccgccggga
    11641 tgaccatcac ccgctccggt gtgatgcgcc gtgcgccgct ggcaatgcgc ctgcgtgcaa
    11701 acagcacctg ggcgatgctg gaatttgtct ttaacggcat ggtgttcctg ctgttaggtc
    11761 tgcagctgcc gggtattctg gagacgtcgc tgatggcagc agaaatcgac cctaacgtcg
    11821 aaatctggat gctgtttacc gatattattc tgatatatgc ggcgctgatg ctggtccgtt
    11881 tcggctggct gtggacgatg aaaaagttca gcaaccgctt cctgaagaag aagccgatgg
    11941 agtttggttc gtggaccaca cgagaaatcc tgatcgcgtc tttcgccggg gtgcgtgggg
    12001 cgatcacttt ggccggtgtg ctctctatcc cgctgctctt gccggatggt aacgtcttcc
    12061 cggcgcgcta tgagctggtg tttctggcgg ctggtgtcat tctcttctcg ctgtttgtcg
    12121 gcgtggtgat gttgcctatt ctgctacaac acattgaagt cgcggaccat tcgcaacaat
    12181 tgaaagaaga acgtattgcg cgagcggcaa cggcagaagt ggcgattgtg gcgatccaga
    12241 aaatggagga gcgtctggcg gcggataccg aagagaatat cgataaccag ctgcttacgg
    12301 aggtcagttc tcgcgtcatt ggtaacctgc gtcgtcgcgc cgatggacgt aacgacgttg
    12361 aaagttccgt gcaggaagag aaccttgagc gtcgcttccg tctggcggca ttgcgttctg
    12421 aacgtgctga actgtaccac ctgcgcgcca cgcgggagat cagcaacgaa acgctgcaaa
    12481 aattactgca cgatctcgat ttgcttgaag cgttgctaat tgaggaaaat cagtaagccg
    12541 aaagcgacgt aaccaggccc gaggtaatct gaaaaaggtg caatcttttg cacctttaac
    12601 aggttagctc gggctaacat tcatgattct aaaacaaata agttatctct cttttaaagt
    12661 catttcattg gctgttcttc gacttaatct caatctgcgt gttgcataat atctgcagta
    12721 atcttcatag ctacttcatg agtattattc tcagtttgtg acatgccagt atgagaggta
    12781 caatcttctt caaagaattt ctgtagtgtt accagggtat cactattaat gggaatattc
    12841 tcctccccac ggagaatgga aatgcccatt ccctggacgc tagcctcatc gaaattaacc
    12901 cgatagagac gggtattaaa catccaggct tttgttaaaa cagaacagtt gaaatggcaa
    12961 ttgtttaaat tggcattatt aaaattaac
//
LOCUS       DAZFDC010000128        12947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFDC010000128 DAZFDC010000000
VERSION     DAZFDC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.128"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(495..1067)
                     /gene="hdeD"
                     /locus_tag="ACHJ48_003849"
     CDS             complement(495..1067)
                     /gene="hdeD"
                     /locus_tag="ACHJ48_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO4709315.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1322..1654
                     /gene="hdeA"
                     /locus_tag="ACHJ48_003850"
     CDS             1322..1654
                     /gene="hdeA"
                     /locus_tag="ACHJ48_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO4709316.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            1770..2096
                     /gene="hdeB"
                     /locus_tag="ACHJ48_003851"
     CDS             1770..2096
                     /gene="hdeB"
                     /locus_tag="ACHJ48_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO4709317.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2160..2807
                     /gene="yhiD"
                     /locus_tag="ACHJ48_003852"
     CDS             2160..2807
                     /gene="yhiD"
                     /locus_tag="ACHJ48_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO4709318.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2859..3629)
                     /locus_tag="ACHJ48_003853"
     CDS             complement(2859..3629)
                     /locus_tag="ACHJ48_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO4709319.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAAHYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(3626..4435)
                     /locus_tag="ACHJ48_003854"
     CDS             complement(3626..4435)
                     /locus_tag="ACHJ48_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO4709320.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLAGIAINALCGAAIGI
                     LSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDE
                     EAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIP
                     CSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(4484..5107)
                     /gene="chuY"
                     /locus_tag="ACHJ48_003855"
     CDS             complement(4484..5107)
                     /gene="chuY"
                     /locus_tag="ACHJ48_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO4709321.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHKTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVRRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(5107..5601)
                     /gene="hutX"
                     /locus_tag="ACHJ48_003856"
     CDS             complement(5107..5601)
                     /gene="hutX"
                     /locus_tag="ACHJ48_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO4709322.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(5614..6951)
                     /gene="hutW"
                     /locus_tag="ACHJ48_003857"
     CDS             complement(5614..6951)
                     /gene="hutW"
                     /locus_tag="ACHJ48_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO4709323.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTASPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLSNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMLIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIMREKP"
     gene            complement(6971..7885)
                     /gene="shuT"
                     /locus_tag="ACHJ48_003858"
     CDS             complement(6971..7885)
                     /gene="shuT"
                     /locus_tag="ACHJ48_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFO4709324.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLQQMYANIRQLAKTLQVP
                     EQGEALVTQISQRLERVQQNVATKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            8568..10550
                     /gene="chuA"
                     /locus_tag="ACHJ48_003859"
                     /gene_synonym="shuA"
     CDS             8568..10550
                     /gene="chuA"
                     /locus_tag="ACHJ48_003859"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFO4709325.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            10599..11627
                     /gene="chuS"
                     /locus_tag="ACHJ48_003860"
     CDS             10599..11627
                     /gene="chuS"
                     /locus_tag="ACHJ48_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFO4709326.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(11688..12218)
                     /gene="dctR"
                     /locus_tag="ACHJ48_003861"
     CDS             complement(11688..12218)
                     /gene="dctR"
                     /locus_tag="ACHJ48_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO4709327.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12374..12940)
                     /gene="slp"
                     /locus_tag="ACHJ48_003862"
     CDS             complement(12374..12940)
                     /gene="slp"
                     /locus_tag="ACHJ48_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO4709328.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3457 a         3009 c         3142 g         3339 t
ORIGIN      
        1 ttaaaaagta atccgattta aatatcgagt ctccttgttt cgacttaagc tggcaattgg
       61 attgccagct ttcttttaac gcattgaaat tacctgagta aataacgcat tcatgtcttc
      121 aggtttttat atagcgtctg gagacatgga tatttatgca atgtaatcaa cgccagttgc
      181 aaaaaaatga acccggatta cttatttaac cgcccatagc atcaacgctt acggaactct
      241 ttgttcactt tcccacagca cttttaaaac attaaacctg actacggaaa atatcagcca
      301 tgataatgtt tgaaatggct aatttgccat ggagtgaaaa aaattagatg aaattcagta
      361 ggttgaaata atcactagca ggtaattatt tcaatgatag tgcgcaattg atctacaaca
      421 ctgcgtagcg gagagagtat taatcggatc atagtcacat caagtgacta tgatccgggt
      481 gacaaccggg ataattattg ctgcttaacg aacaaactgg cgaagctgaa caggctggcg
      541 gcgctaaata tcagttcaat tcccaccagt gtggaaacca gcgttacaga caccatcggc
      601 gttgcaccaa ggaatatcca ggcaatgacg atatccagca caccaataac gagctgtagc
      661 cagctgcctt tcattgaacg ctgacgatac caactcatca ggcgaataac ccctgcaaca
      721 cagaacaaac cggcaataaa tgccgcaatg gcaaaaatgc ccagctccgg tgcgcggatg
      781 aagaaatagc cgatcaataa ataggcgact gcgacgagga aaccggataa taccggccag
      841 aaattatgac tgcggttgct gaataatcca acaataagcg caatgcccga acagattaat
      901 aatgcaccta ctactgtgct taaaatatcg ccagaaacga acgggaaact gatacacagc
      961 aacccgacga taaacagcag cacggcaata aactggattg ctctgcgatg ctttttcagc
     1021 atctccagat caaacttcaa aattgttgcc ttatctatat ataacataga accaccctat
     1081 aaaattaaga agaaaatccc ctgctatcaa tctatgccaa aaacgcgtct aagaatgcag
     1141 tcgatttaat aaaaatttcc taattgcagt atctgatgca tctgtaactc attgtattga
     1201 aataaaaata tctgattttg atattttcca tcaacatgac atatacagaa aaccaggtta
     1261 taacctcagt gtcgaaattg attcgtgacg gctctttcac tttatagttg aggatattac
     1321 gatgaaaaaa gtattaggcg ttattcttgg tggtctgctt cttctgccag ttgtgagcaa
     1381 tgcagcggat gcgcaaaaag cagctgataa caaaaaaccg gtcaactcct ggacctgtga
     1441 agatttcctg gctgtggacg aatccttcca gccaactgca gttggttttg ctgaagcgct
     1501 gaacaacaaa gataaaccag aagatgcggt tttagatgtt cagggtattg caaccgtaac
     1561 cccagctatc gttcaggctt gtactcagga taaacaagcc aactttaaag ataaagttaa
     1621 aggcgaatgg gacaaaatta agaaagatat gtaattccgg gaatgcgtta catcgtactt
     1681 ccttgcatat tgaacaggcc ggaatatctt ctttaaaagc agctattcct cctgttcata
     1741 tataatctct atattgaatg ggttacaaaa taaatatttc atctctccgt aaagcgttta
     1801 tttttatggg cgctgtagcg gctttgtcac tggtgaacgc acaatctgcg ctggcagcca
     1861 atgaatccgc taaagatatg acctgccagg aatttattga tctgaatcca aaagcaatga
     1921 ccccggttgc atggtggatg ctgcatgaag aaacagtata taaaggtggc gatacagtta
     1981 ctttaaatga aaccgatctc actcaaattc ctaaagtgat cgaatactgt aagaaaaacc
     2041 cgcagaaaaa tttgtatacc ttcaaaaatc aagcatctaa tgacttgccg aattaatgag
     2101 gtacaagtaa aaaggagtgg caagttgagc catcttgctg ctcctttttg catttttata
     2161 tgacagcaga atttattata cgtcttatac tcgcggcaat tgcctgtggc gctattggca
     2221 tggaaaggca aatgcgcggc aaaggagcag ggttacgcac tcatgtatta attggcatgg
     2281 gaagcgcact gtttatgatt gtttcgaaat atggttttgc tgacgtgctg tctttagatc
     2341 acgtcggact ggaccccagc cgtattgctg ctcaggtggt gacgggcgtc gggtttatcg
     2401 gtgcaggtaa cattctcgtt cgtaaccaaa atattgtagg tttgacgacg gcagcggata
     2461 tctgggtgac cgccgccata ggtatggtta ttggcagtgg tatgtacgaa ctgggtattt
     2521 atggttcagt gatgaccttg ctggtgctgg aagtcttcca tcaattaacc ttccgcctga
     2581 tgaacaaaaa ttaccattta cagctgacat tagtgaacgg taatacggta tcaatgctcg
     2641 actggtttaa gcagcaaaaa ataaaaactg atttagtttc gttgcaggaa aatgaagatc
     2701 atgaagtggt cgccattgat atccagcttc atgccacaac gtcgatagaa gatctgttgc
     2761 gattattaaa aggtatggca ggggtgaaag gcgtttcaat tagctaatct gtatagttat
     2821 ttattcgcag gagtaccagt gcggtactcc tgttgctttt aatgatcgag gacgatcagt
     2881 ggtgaatggt tggcaggatc tttaagtacg gtaatatccg cgccgtagag cattgtcagc
     2941 gcctgctgtg tcaatacgtc ctgtggttta ccatttgcga taacctttcc tttttgcatt
     3001 aatacaacac ggtctgcata atgcgcggca agatttagat cgtgcaatat gcagcagaca
     3061 ttgaactgcc gctcgtgaac taactggcgt aataaacgaa acaagtgctg ctgatggtga
     3121 atatccagcg ccgaagttgg ttcatcaaga aataaccatt tcggtgaggg agttggttcc
     3181 cagagttgca ccagcaggcg cgctaattgc acgcgctgtt gttcaccacc ggataattgc
     3241 cgatagtcgc ggtttgctaa cgcctgacag tcacataacg ccattatttg tgctgtttca
     3301 ttatcctgat taccagtacg atgcggatgc cgacccatct ggataacttc ttgtacgctg
     3361 aatggaaacg ccatgtggct attttgacgc atcacggccc ggtgtttcgc cagttcagta
     3421 atcgaccatt catttaatgg cttattaaac aaccgacatt cgccgctatc aggttgaaga
     3481 tagccggtta actggcgaag cagcgtagat tttcccgcac cattagggcc gagaatggca
     3541 acaatttccc caccgggaaa agtcagcgag acattatcag tcagacgccg cccctgaagg
     3601 ctataaacca gattctgggc gctgatcatg tgatcctccg atttcgcaga atcaaccaca
     3661 taaaataagg gccaccaagc aggctggtta ataatcccac gggcatttct gctggctgta
     3721 ccagcgtgcg ggcaagcgtg tctgccatca gcaataaaca ggctccggcg agggcggaac
     3781 aagggatgag ccagcggtga tttgctccgg tagtcatgcg aatcagatgc gggatcacca
     3841 ggccaataaa gccgataatg ccgcttaccg atacggcagc accaacgagc agtgagctca
     3901 ctaatagtaa ttgctggcgt ttacgcttaa cgttcacgcc gaggtaatgg gcttcttcat
     3961 caccgagctg cagtaaattc agcgttccgg cgagacaagt tgttgcgata atggccggaa
     4021 ggatgaatga actggcaacc aataacgtcg gccattgcgc ctgtccaaga ttgcccatca
     4081 tccacaatgt gagttgtcgt agctgctgct catcgccaat atagctcagg atgccgatcg
     4141 ctgcgccgca cagggcattg atggcgatac cggcgagtaa tcctgggtca gccattgggt
     4201 tgcgaaaaag cccctgcata attacccctg cggttgccaa cgctgcacct accagcaccg
     4261 ccagcaaaac ccgtggtaga cgaatattga gccagatatc gcggtattcc tcatcaaaaa
     4321 gggcatcaaa actgatcttt aacgctccct gactagcacc gaacagcacc agggcaagca
     4381 ataatagtga tagtcccata aagaccctgg cggatgaaaa tgaatccttg agcacgcaaa
     4441 actccgttac gacattacgg cgcggacatg ccgcgccggg gtatcacgcc ggtttcaggt
     4501 ccggttcaat cagactgtag acctgctggt taagtgctgg cgcatttgcc agttcatgga
     4561 tatgtgcggc gacatcggca cggcggacaa aaccatgaca ctcctgattt tgtatccgtt
     4621 gtgctttgcc tgttgctgcg ccgtcaagca gaccaccagg gcggagaatg gcgtagtcta
     4681 gctggctggt ttgcagccag ctttccgcca gcgttttttc ccgtaccgcc tgaccaaacg
     4741 cagcctttgc ccgctccgat aaaaacggcc agctatcgcc gcatcccaaa gaggtcacca
     4801 gaatcatgcg actgatgccg gccttttcgg cctcatcaat cactgttttg tgcgccagat
     4861 aatcctgagc tcctcccatt gttgagatga taagcgcatc cgggccagct gcgcggcaag
     4921 cagccgcgac tacgctggca tcacaggcgt cgcctgtaaa aacctgtacg ccttgttgcg
     4981 ccagtttcgt ggcggcatcg gcatgacgaa tgacagcaac gaccggacgt tgttccgcca
     5041 gcgccagttc aagcgttctg gcacccacac cttttccgcc tgcgccaaag agtagccacg
     5101 gcgtcatcag gcgtgttcct ttaacgatgc cgccagagta tggaaggcgc tgacttgttc
     5161 gctcaggagc tgacgatggt catccctgcc gaggaagatt ttcagcatgg cgctgccttc
     5221 tttgttgaaa aagagaatgg acgcggtgtc catgcccata aatttacgtt cgattaaggc
     5281 aatatgcgtg cagttttctg ctttgatatg cccggacatg ccgtgctttc cgcgtaggtt
     5341 gaaatagccg tggcgatgga aacccgaagg cagttcgccg ctaaattcga ggattacatc
     5401 ggcagtatgt actaacgtgg tgacgttgcc ccattcgcag acggtatccc agacagtatc
     5461 gaatttatcg cctggcacta cggtagagga gggtagattt ctcactacct ccagcagtgt
     5521 ggtgttgtat tgttcggcta ccacctccag cgtaccgtcc ggctccgttt tcaaaaattc
     5581 ttgtaacgag acatggctca taaatcactc ctgttatggt ttttcacgca taatcgctgg
     5641 cgcattaagt acctgaatta gttcattaag agactgcaaa atattgcttg cccagaaacg
     5701 accttcatta gtcagccgca ggcaagttga agcatcgcga cttaagcctt tttggtgcca
     5761 ttgagccagt aacggcgcga gtttttcggc atgtggcgta agttcatcca gcggtacgcg
     5821 cgctgtttca acacctgact gcaaggtatg acgccattgc gcattacgtt cggcgttacg
     5881 catgatcaac atcagcggtt ttttgcctgc cgcgacggat tcatgccagg tctgaagatt
     5941 gcgttcgttc atccaggagt aaccattaat cgaaccgccg gctccggaac caaaggccag
     6001 acaatcggca ccttgtttaa tcagcaggtt atagagattg cgttcgcgtg tggtacggcc
     6061 ccagtggctg ttactgatgc agcgccaacc ggcatcgtcc ataaaatcac atccttgcag
     6121 ataaagatcg cgacgttctg ccggtgaggg tacggtagta cgcccatttt ccacggcctt
     6181 gcccagcggt gtattggaca ggacattgag cgcatagaga tcgacgccgt cgagaccgat
     6241 atcgcgggca atagccagat cttctcccca ggtttgcgca tcctgacccg gcaggccaaa
     6301 cagcaggtca cagaccaccg cagcacggtc acgtttgacc aggctttcca taaacgcaat
     6361 agcggttggg ccatccgagg tgcgggccat tttcttgcgg attttgctgt taaacgactg
     6421 gataccaatt gagaagcggt ttgctcctgc atcaaggcaa gcatcgattc gctcagcgtc
     6481 aaaattcaat acccgtcctt cgatagtgat ttcacaatca ggggccagtg gcagcttttc
     6541 tcgtaatgtg gtgatgattc tggccaaatc gtgtgccgaa agtgcggaag gcgtaccgcc
     6601 accgaaatag accgcgtgga tgggggcaga ctgatgcaat acgctgtccg cttccatttc
     6661 tatttcacga atcagggcgt cggtataatg agcacacgca tcttcattaa aacggttctg
     6721 ataaaaacca caaaacgtgc agtgtgtcgc acaaaaagga atgtgtaggt aaaccagtcg
     6781 cttacgaggt gatgccgttt gattgatcat ctcttgccag gtctgtgcca gttgctcttt
     6841 ggcaaccgga atggctccac gaaacggcat catggcgcgc ctgtctttaa atggctgatc
     6901 accgtctaaa gcaaaatgtg gcgtgagatc cagggtgtta tttgcgttca ttcgtctgat
     6961 ttaaatccat ttattgcggc caaagctgct ggtgtaaaga ctcaacgaca tcagcaatac
     7021 gcggacccat tcccagaatc aggttttgat caacggtgat aatgcgctgg tttttccatg
     7081 cggcagtgtg ggtaattcca gcaatagaac gtagccgatt aatatcgcca tcgaccattt
     7141 gtgaggttac gacaatcact tctggattag ccgcaatcag tgattctgca ctgtagcttt
     7201 tgtactgctg gtgggtagcg acgttttctg ctccggcaag cgaaagaatg gcgtccgcga
     7261 cgctgccttt accggcaact tgtggcgcac ttccgcctgc ggagagaata aacatggctt
     7321 taaccggggc ttttttggtc gccacgtttt gctgtactcg ctccaggcgt tggctgattt
     7381 gtgtcaccag cgcctcgcct tgttcaggaa cctgtagcgt ttttgccagt tggcgaatgt
     7441 tggcgtacat ctgctgaagg gtggcaggca cacgcggcag ggtgacgacg ttgactttct
     7501 gcgctcgtag ttggtcgagc acaatttgtg gcccggcatc ctgccaggta attacgctat
     7561 ccgggcgaag tgacaaaatg ccttcgctgc tgagttgttt ccagtagcca atatgcggca
     7621 gtttggcggt ttctggtgga taagatgtcg tttcatcgac accaaccacg cgttcgccag
     7681 cgcccatcgc gtagattagc tccgtcagcg atcctcctgc gaccacgata cgttcggcag
     7741 ccgttacgct aaaagcccag ctaagagcca gtactgccgt aaacaatttt ttacgtttta
     7801 ctgcggtctt cgcggcagag gccacggctg aaatacataa cgtcagtggg gaaaaaagaa
     7861 acggcctggt gatgatcctt ggcataatat gagaactctt atcaaattga gaatgatcat
     7921 gattatcatt tgagttatat atgaaataca accgtcgatt caatacgcaa ggcgttacaa
     7981 gcgtatttat ttttgatttt tatcaagatg cttcgtcagc taacgttctt gtttccgact
     8041 aaatttcatc gcgggaaaaa gctgagaacc cagagatatc gaggcttgca gagcaaagag
     8101 aagaaaaaat tatcactgtt aacgacaaaa tttagtgatg aggcatgggg ggataaccat
     8161 aaaaacaagg tggtttacag ggcgcaggat aggtttcata accccctgtt tattgtgtta
     8221 atggtggtta aaaaaaaact tgatcataaa aaattgatcg ataataattc tcattatcac
     8281 gtttgcattt aacaaaacac gaccatatgg ataacaaaac catagcattt gcaagggata
     8341 acgcacagct ctcttcactc ctgatggggc atatcgtttt gcctgacaaa gagggcgtgc
     8401 ttttgcgtaa ttgccgagtt atcaggcaat ttcatgggat ataaacgctt gtaaactcac
     8461 ccggcgaata ttatcgctct gaattttaaa taaatttatt tttcgccagc caccccggta
     8521 ttaacccagg cagccagcat tttttatttt cctcatggag aatcgctatg tcacgtccgc
     8581 aatttacctc gttgcgtttg agtttattgg ccttagctgt ttctgccacc ttgccaacgt
     8641 ttgcttttgc tactgaaacc atgaccgtta cggcaacggg gaatgcccgt agttccttcg
     8701 aagcgcctat gatggtcagc gtcatcgaca cttccgctcc tgaaaatcaa acggctactt
     8761 cagccaccga tctgctgcgt catgttcctg gaattactct ggatggtacc ggacgaacca
     8821 acggtcagga tgtaaatatg cgtggctatg atcatcgcgg cgtgctggtt cttgtcgatg
     8881 gtgttcgtca gggaacggat accggacacc tgaatggcac ttttctcgat ccggcgctga
     8941 tcaagcgtgt tgagattgtt cgtggacctt cagcattact gtatggcagt ggcgcgctgg
     9001 gtggagtgat ctcctacgat acggtcgatg caaaagattt attgcaggaa ggacaaagca
     9061 gtggttttcg tgtctttggt actggcggca cgggggacca tagcctggga ttaggcgcga
     9121 gcgcgtttgg gcgaactgaa aatctggatg gtattgtggc ctggtccagt cgcgatcggg
     9181 gtgatttacg ccagagcaat ggtgaaaccg cgccgaatga cgagtccatt aataacatgc
     9241 tggcgaaagg gacctggcaa attgattcag cccagtctct gagcggttta gtgcgttact
     9301 acaacaacga cgcgcgtgaa ccaaaaaatc cgcagaccgt tgaggcttct gaaagcagca
     9361 acccgatggt tgatcgttca acaattcaac gcgatgcgca gctttcttat aaactcgccc
     9421 cgcagggcaa cgactggtta aatgcagatg caaaaattta ttggtcggaa gtccgtatta
     9481 atgcgcaaaa cacggggagt tccggcgagt atcgtgaaca gataacaaaa ggagccaggc
     9541 tggagaaccg ttccactctc tttgccgaca gtttcgcttc tcacttactg acatatggcg
     9601 gtgagtatta tcgtcaggaa caacatccgg gcggcgcgac gacgggcttc ccgcaagcaa
     9661 aaatcgattt tagctccggc tggctacagg atgagatcac cttacgcgat ctgccgatta
     9721 ccctgcttgg cggaacccgc tatgacagtt atcgcggtag cagtgacggt tacaaagatg
     9781 ttgatgccga caaatggtca tctcgtgcgg ggatgactat caatccgact aactggctga
     9841 tgttatttgg ctcatatgcc caggcattcc gcgccccgac gatgggcgaa atgtataacg
     9901 attctaagca cttctcgatt ggtcgcttct ataccaacta ttgggtgcca aacccgaact
     9961 tacgtccgga aactaacgaa actcaggagt acggttttgg gctgcgtttt gatgacctga
    10021 tgttgtccaa tgatgctctg gaatttaaag ccagctactt tgataccaaa gcgaaggatt
    10081 acatctccac gaccgtcgat ttcgcggcgg cgacgactat gtcgtataac gtcccgaacg
    10141 ccaaaatctg gggctgggat gtgatgacga aatataccac tgatctgttt agccttgatg
    10201 tggcctataa ccgtacccgc ggcaaagaca ccgataccgg cgaatacatc tccagcatta
    10261 acccggatac tgttaccagc actctgaata ttccgatcgc tcacagtggc ttctctgttg
    10321 ggtgggttgg tacgtttgcc gatcgctcaa cacatatcag cagcagttac agcaaacaac
    10381 caggctatgg cgtgaatgat ttctacgtca gttatcaagg acaacaggcg ctcaaaggta
    10441 tgaccactac tttggtgttg ggtaacgctt tcgacaaaga gtactggtcg ccgcaaggca
    10501 tcccacagga tggtcgtaac ggaaaaattt tcgtgagtta tcaatggtaa tcatctgccc
    10561 cgatatttcg gggcatttat ctggaaggaa gagagacaat gaaccactac acacgctggc
    10621 ttgagttaaa agaacaaaat cccggaaagt acgcgcgtga catcgcaggg ttaatgaata
    10681 ttagagaagc agaactggca tttgcacgcg tcacgcacga tgcgtggcgg atgcacggcg
    10741 atatccgtga aattctggcg gcgctcgaaa gtgttggcga aaccaaatgt atttgtcgta
    10801 atgaatatgc agtccatgag caagttggta cgttcacaaa ccagcatttg aacggacatg
    10861 ccggattgat cctcaatccg cgcgcgctgg atttacgtct gtttctcaat caatgggcca
    10921 gtgttttcca catcaaagaa aacacggctc gtggcgaacg ccagagtatt cagttctttg
    10981 atcatcaggg cgatgcatta ctaaaagttt atgccaccga caataccgat atggcggcat
    11041 ggagtgagct tctggcacgg tttatcaccg atgagaatac gccgcttgag ttaaaagccg
    11101 ttgatgcgcc agttgttcaa acgcgagccg atgccactgt ggtcgagcaa gagtggcggg
    11161 cgatgaccga cgttcatcag ttttttacgt tgctcaagcg ccacaacctg acgcgccaac
    11221 aggcgttcaa tctggtggca gacgatttgg cctgcaaagt atccaacagt gcgttggcgc
    11281 aaattcttga atctgcacag caggatggta atgaaatcat ggtgtttgtt ggcaaccgtg
    11341 gctgcgtaca gattttcacc ggtgtggtag aaaaagtggt gccaatgaaa ggttggctga
    11401 atattttcaa cccgacgttt actcttcatc tattagaaga gagcattgct gaagcctggg
    11461 ttacccgtaa accgaccagc gatggctacg taaccagtct ggaattgttt gcccatgatg
    11521 gtacgcagat agcgcaactt tatggtcaac gtacagaagg cgaacaggag caagcgcaat
    11581 ggcgtaagca aattgcttcg ctgataccgg aaggcgttgc tgcataacgt ataattaacg
    11641 ggaggtctgg ctatttattg ctgggcctct ttatattgat caatcgttca caccagataa
    11701 tcaatatgct gatggcgtac cagctcattg atccgcttaa gattgaggat catcatgata
    11761 ttgtgtttat ggcagtagaa cgtttttagc gatattttca gtttctccag aatatcttcc
    11821 gttgtcattt gattcgcaat gcagctcaga acctgatgtt gcttgcgcga aagtgacaca
    11881 ttcgcttcag gcacagagca gtaactgcca ttgatgagtg cgacaaaatc aatgatagtg
    11941 atagtccgcg gaacaaaggt caccggcact ttccccaggc agcgtttaat attaaagggg
    12001 gagaaaataa tgacatggac aggttttaat cgttcaattt gagtgagaaa attagaatga
    12061 aatacattat tcattaatgt atcaataatg acgaaggcat tctgatgcaa cattacgtcg
    12121 atctcttctt cgttctgtat atgaacgaca ttacctttac tcagaatgtt tttcatcgcg
    12181 gtgaagaaca tcgtatccct ggtaattata agaaacataa ccgactcctg gtgcggacat
    12241 taactttata atcagtttgc caaaggtaat taacaggaca gggcttataa taataatagt
    12301 tagcttactg tgtattaatt tgatgtaatg catgatgcag atgctgagaa agcatctgca
    12361 tctttcggtg gtgttatttg accagctcag gtgttacctg actcaccgca ttggtgtagt
    12421 aaggcgcacc ccagcccggt tccggccaga atgcaccata gccgtaatcc cacaggttat
    12481 aggtggtatt aaccacttct ctcaaatgcc acacctggat gccctgcata ttcacttcca
    12541 ggaagttata cgggacttta ttgataaagc caggttgttc accctgaatg gtgccgagga
    12601 tggtaacaaa gtgattacga tagttcactg gatcaaggaa gccgctttgt ctggcgagca
    12661 gtcggccctg atagttggct tcaatatcag gcttcgcata gctatccaac ggtaatacag
    12721 cgatttctaa caacgtatcc gttttgccat tgataacgtt gataaccttc ccaccaaagc
    12781 gcgcttgttg accaacatat aaccccggct ggttatgaac agcaacaaaa cttttttgaa
    12841 tatcaggttg gttatttcct ttgatatttt gcggaattga actacatgct gcaagcaaaa
    12901 atgaaaggct gagtatgagt gcaccttttg tcatgttcat gttacta
//
LOCUS       DAZFDC010000129        11825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFDC010000129 DAZFDC010000000
VERSION     DAZFDC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.129"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            110..760
                     /gene="csgD"
                     /locus_tag="ACHJ48_003863"
     CDS             110..760
                     /gene="csgD"
                     /locus_tag="ACHJ48_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO4709329.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            765..1154
                     /gene="csgE"
                     /locus_tag="ACHJ48_003864"
     CDS             765..1154
                     /gene="csgE"
                     /locus_tag="ACHJ48_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO4709330.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1179..1595
                     /gene="csgF"
                     /locus_tag="ACHJ48_003865"
     CDS             1179..1595
                     /gene="csgF"
                     /locus_tag="ACHJ48_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO4709331.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1622..2455
                     /gene="csgG"
                     /locus_tag="ACHJ48_003866"
     CDS             1622..2455
                     /gene="csgG"
                     /locus_tag="ACHJ48_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO4709332.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2519..3010)
                     /gene="ycdZ"
                     /locus_tag="ACHJ48_003867"
     CDS             complement(2519..3010)
                     /gene="ycdZ"
                     /locus_tag="ACHJ48_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO4709333.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILSYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTALREQEIK
                     NKA"
     gene            complement(3112..3666)
                     /gene="ycdY"
                     /locus_tag="ACHJ48_003868"
     CDS             complement(3112..3666)
                     /gene="ycdY"
                     /locus_tag="ACHJ48_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO4709334.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3690..4427)
                     /gene="ycdX"
                     /locus_tag="ACHJ48_003869"
     CDS             complement(3690..4427)
                     /gene="ycdX"
                     /locus_tag="ACHJ48_003869"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO4709335.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4482..5420)
                     /gene="ghrA"
                     /locus_tag="ACHJ48_003870"
     CDS             complement(4482..5420)
                     /gene="ghrA"
                     /locus_tag="ACHJ48_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO4709336.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALA
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGWEELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5654..5741
                     /locus_tag="ACHJ48_003871"
     tRNA            5654..5741
                     /locus_tag="ACHJ48_003871"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5688..5690,aa:Ser,seq:gga)
     gene            complement(5891..6733)
                     /locus_tag="ACHJ48_003872"
     CDS             complement(5891..6733)
                     /locus_tag="ACHJ48_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO4709337.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6818..7015)
                     /locus_tag="ACHJ48_003873"
     CDS             complement(6818..7015)
                     /locus_tag="ACHJ48_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO4709338.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7027..7518)
                     /locus_tag="ACHJ48_003874"
     CDS             complement(7027..7518)
                     /locus_tag="ACHJ48_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO4709339.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGNGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7515..7889)
                     /locus_tag="ACHJ48_003875"
     CDS             complement(7515..7889)
                     /locus_tag="ACHJ48_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO4709340.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7936..8310)
                     /locus_tag="ACHJ48_003876"
     CDS             complement(7936..8310)
                     /locus_tag="ACHJ48_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO4709341.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8473..8694)
                     /locus_tag="ACHJ48_003877"
     CDS             complement(8473..8694)
                     /locus_tag="ACHJ48_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO4709342.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8757..9233)
                     /locus_tag="ACHJ48_003878"
     CDS             complement(8757..9233)
                     /locus_tag="ACHJ48_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO4709343.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9249..9734)
                     /locus_tag="ACHJ48_003879"
     CDS             complement(9249..9734)
                     /locus_tag="ACHJ48_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO4709344.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9825..10643)
                     /locus_tag="ACHJ48_003880"
     CDS             complement(9825..10643)
                     /locus_tag="ACHJ48_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO4709345.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(10983..>11825)
                     /locus_tag="ACHJ48_003881"
     CDS             complement(10983..>11825)
                     /locus_tag="ACHJ48_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO4709346.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNT
                     PVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSF
                     TSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLALRNAM
                     ARIK"
BASE COUNT         2968 a         2990 c         2944 g         2923 t
ORIGIN      
        1 gctgatttat tactacacac agcagtgcaa catctgtcag tacttctggt gcttctattt
       61 tagaggcagc tgtcaggtgt gcgatcaata aaaaaagcgg ggtttcatca tgtttaatga
      121 agtccatagt attcatggtc atacattatt gttgatcact aaaccttctt tgcaggcgac
      181 agctctcttg cagcacctta aacaatcgct ggcaattaca ggaaaattac ataatattca
      241 acgttctctg gacgatatct cttcaggctc tattattctt ctggatatga tggaagcgga
      301 taaaaagctt atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat
      361 attgttgcta aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat
      421 caacggcgtt ttttatgcca tggaggatca agaacgcgtt gtcaatggat tgcaaggcgt
      481 cctgcgcggc gaatgctact ttacgcaaaa gcttgccagt tacctgatta cacattctgg
      541 taactatcgt tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct
      601 gaataagctg cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga
      661 aaatacggtt aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac
      721 acaagcggtt tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac
      781 gctggattgt ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag
      841 aagtcccggg attgttaact gaccatactg tttcatctat tggccatgat ttttaccgag
      901 cctttagtga taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca
      961 gtgcacgatg gggaagctgg atcacaataa cggtcaatca ggacgttatt ttccagactt
     1021 ttttattccc gttgaaaaga gacttcgaaa aaactgtcgt ctttgcactg attcaaactg
     1081 aagaagcact aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc
     1141 atgatgaatt ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag
     1201 ttctactcat gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta
     1261 atccaaactt tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc
     1321 aaaactctta taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt
     1381 tagataactt tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta
     1441 ataccggtaa accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg
     1501 atggtcaatt gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg
     1561 tttcgggttt acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat
     1621 catgcagcgc ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc
     1681 gcctaaagaa gccgccagac cgacattaat gcctcgtgct cagagctaca aagatttgac
     1741 ccatctgcca gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac
     1801 cgggcaattt aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac
     1861 ggcgatgctg gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgccaggg
     1921 cttacaaaac ctgcttaatg agcgcaagat tattcgtgcg gcacaagaaa acggcacggt
     1981 tgccattaat aaccgaatcc cgctgcaatc tttaactgcg gcaaatatca tggttgaagg
     2041 ttcgattatc ggttatgaaa gcaatgtcaa atctggcggg gttggggcaa gatattttgg
     2101 gatcggtgcc gacacgcaat accagctcga tcagattgcc gtgaacctgc gcgtcgtcaa
     2161 tgtgagtacc ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga
     2221 agttcaggcc ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg
     2281 ttacacctcg aacgaacccg ttatgctgtg cctgatgtcg gctatcgaaa caggggtcat
     2341 tttcctgatt aatgatggta ttgaccgtgg tctgtgggat ttgcagaaca aagcagaacg
     2401 gcagaatgac attctggtga aataccgcca tatgtcggtt ccaccggaat cctgacagat
     2461 aaaaatgccg gaattaataa tccggcattt actgtttaag cgaaagcgtg agtgcccctc
     2521 acgctttatt tttgatttcc tgctcacgga gagccgtctt tgcactacgc gccgccagcc
     2581 acaggccgct gtttttcatt gcgtaaccaa atatcagccc aagcgccagc gaaggtaaca
     2641 ccagtttcca gtcaccctgc ccggcaaacg tcgcgcatgc gcctataaac gtccccggaa
     2701 caaatgaaag cagcagctgt ttggcctgaa tacacatcag aaacgcgaca ataccggtta
     2761 tgacataact gagaatttcc agatgtggcg ccagtgcact accgtaaata atgaccatcg
     2821 cccacaccac gccactaagc agagttgcag cggagatcgc cagccctttc agcccacctt
     2881 gcgggcaggc aaagtaggcc gtacagccca ggaagcccgc ccagcttagt aagcccaggg
     2941 aaacagccac ccatccccag ataccggaga gaatgcctgt tgtgattgca atagagagaa
     3001 gtatgttcat gccgcgcatg atagcagaaa acgcggccat gaatgagatc gagcacacat
     3061 tttatgcaac agcaaattat ctgttgcagt tatgagtgac gcatatcaca tttactcttc
     3121 agaatcttct tccagctcgt cccacattgc actaatggca tcgcgggtta gcggtgccat
     3181 ggtgcgccag aaaggcgtgg ttgcatgggc ctccactttg ccaaggaacg caccacacca
     3241 gggtaacaga tactcactga acagtgtttc cagtgcttcg ctctcatctt ccgttgactg
     3301 atcttccagc caggaagccg caagcagcaa tgtgccgatg tgatcggcag gcgtatccgc
     3361 taatggcatc cctcgttcag aaagaaaagc gcgcacttcc gcttccgttg cgtcctcaac
     3421 ccatgcgcta cgatatggcg gcacggcaca ttcatcgccg ataaacaacg cattgtaatc
     3481 ggcagagact tgcgtcatat cacagctttt ctgcaaacgt gtcagcaact catcctgctc
     3541 cagtggccag ttcgcagcca gtttcccctc acgaatcagg gtaaacagcg gcaccagtaa
     3601 aggatcttgc ggttggcggt aatagagcga acccagcaca cgacagagga tagaaaactc
     3661 gttcattaat atatttccat tactagtaat taaaggtctg caaattccgc aatcggtgcc
     3721 ataccgcgag attcaaggaa gttcagtaag cggcgcggag aaacattcaa aatgcgctct
     3781 ggcggaaaat ctaccgcgtc gaggatttta agacactctt caaattcccc catggtaaac
     3841 gcggtgtgag aatccgagcc taatgccacc caacctccag catcacgtac cgctgcggct
     3901 accgcacgac agttgtcttc actgccctta cgtgagtgta aaaatgagga attattgatt
     3961 tccagcgcca cctgatgttt cgcggctgct tcagcaacgg ctttaacatc tatctcatat
     4021 ttcggatttc cgggatggct tattatatgc acattgccgc tggcgatagt ggcgatcatc
     4081 gcttgtgtat tggtcgcttt gtcatgtggt gcaaaaaccg gctcatgaaa accggcaata
     4141 atcagatcca gcgagtcaaa cattttaccg ctgcaatcaa tttcaccatc aacattttta
     4201 atgttagctt cgatgccgcg caggatccct accccatcaa ccactcgcgg ccagatacgc
     4261 atgttaatga agtgccagtg atgcggcgca tcttccatgt ccgggccatg atcggtaatc
     4321 gcaaaaagtt taatgccctt ttgtttggcc tgggcaatgt aatcactcaa tgtgctatat
     4381 gcatgtgtgc tggcaacggt atgcatatga aggtcgacgg gatacataac tctctcctgt
     4441 attcaattcc cgccaaggat agcaggaatc ctgatgcttt attagtagcc gcgtgcgcgg
     4501 tcgacttgcc cgcagaccct ctcccctttt tcgagctggg caatagtgcg agaaatgtac
     4561 tccacagctt cagcgggacg ggtaatcgcg gcgacatgtg gtgttatcgt cacgcgtgga
     4621 tgttgccaga gcggactttc aggcggtaag ggttcacgat taaaaacatc caacattgcg
     4681 cctttaactt tgccgctatc cagcgccgcg agcaggtcat cttccacgac atgaacacca
     4741 cgcgccaggt tgaggagata cgcgccatcc ggtaattttt cgagtaattg ttgattaata
     4801 atgccgacgg tttcaggggt attcggtaac aaattaatca atacccgaca ttggctcaga
     4861 aatgcagaca gttcttccca tccggcaaag ctttgcacgc caggccacga tttacgggtt
     4921 cgactccagc aacgcagcgg aaagcgccag gtttgcagac tctgagcaac tttactgccc
     4981 agtacgcctg cgcccaaaat gccgatggta aaatcttccc gatgatattc aggcagcggt
     5041 tgccaatgcg aactattttg ctggatgcga taatcgtcaa aacgtcgaaa ccaatgcagc
     5101 acctgactga cagcatattc ctgcatttgc tcgcccatac cggtatcttc caggcgaaaa
     5161 agtggaacag aaggcttcag catttcaggg tgtgcctgta gcttgctcaa aatagaatca
     5221 acaccggccc cgagtgcgaa caccgcttta agatcgcgcc ctgccagcat ttcaacagga
     5281 ggatgccagg ctaaagcata atcagcagag tcattatctc cgcttttcca tgctctgact
     5341 cttgcctgag gaatagcttt gcgcagtgcc tctatccacc attgggtatc gaacgttggg
     5401 tgataaaaga tgatatccat actgactccc gaaaagcgtt gtgcgaaatt tattcgcact
     5461 tatcgttatg atctacaaag accaccagca taacaaatcc gtggtcggtg gccaaaaaag
     5521 cagattttgc ttattaaaac tgcatattga ttgaagtttg aataaacgcg cgatttttta
     5581 aaaaagtttg ttgacctcag gtcatgattt ccctaaatta gcgcccgttc cagcaagaca
     5641 ggaacgacaa tttggtgagg tgtccgagtg gctgaaggag cacgcctgga aagtgtgtat
     5701 acggcaacgt atcgggggtt cgaatccccc cctcaccgcc agattataag cgcatgttat
     5761 tgtttaacat gcgctttttt attttccctt ccagacagta tgccatcggg tatgccatct
     5821 gaaaccggtt tatatgcccc gcgatcatat cttatcggac aatttccgat ctttgagcat
     5881 actggcaaag ctatactgcc agcatatcag gatagtgttt tgcgataata tcattcagtc
     5941 tgtcaaccag ttctggtttt ctgaacgtga tatgcgcgga gcccttctta aagtatctga
     6001 tgctgaacat ctcatcttca tagctctctt taccctgaac agactgaatg tgatcatcca
     6061 gacgaagggt gatgtttccc cggttgtcgg ggattggttt accgctgaac aggtgcaaca
     6121 ttctttccag atcggcgagt cggtcagcct gttgtccggt gataagatga aatccccacc
     6181 ggtcccatct caccagattg ttgacgataa ttttactgcc aaatttacag gggcaattgg
     6241 ttttgtaatt ccagttcagc cctctgaaga cgttgatcac ccctcgctca aacacctcat
     6301 ccttattctg gtgcagttgt tcaaatgtgc tcagaatgtt cgcttcgctg atttccggaa
     6361 aattatcgta ctccagtgac cggtaccatg tatcacgggc ctgcgtatcc attaatgaca
     6421 gcatcccgga acgttgcatc aggtcacgcc agatttcgcg atcaagattc cgggtaatgg
     6481 ctttcattgc tgtttcgggt ttctccatca gccagcagcc gcagcgaaaa tcctgtttca
     6541 tggcccagtc cggggcagtt ttacctccga tgctttccgt cagcaccgag atttcagcca
     6601 gttgtcggat aagtgtgtcg atttgcaaca gtgtactctt tcgtacagcc agaatgtggc
     6661 gaatgctggt tgagcagata acgtccgtat gcccgttgac cacttccgga cgggagattg
     6721 tgatatctga cataatcgat attccttttt gcagataaat gaaaacgcta gccctgtgca
     6781 ggctggcgtc agaggaatga agtctggtga gtgagcttca ccggttctgg tgcagaagct
     6841 gcagatgacg cagggtacgg acatcctccc gggcctgttc gatacagggc aacagtgctg
     6901 ctgaatccgt tttatcctca tcgttgtcaa agataattcc cgatccgcag tcgatattgt
     6961 cctgcagcca cgcaatcaga atatccagcg ctgtttccgt ggttaatgat ttcatgttgt
     7021 tcctggttac cagtcgaaag tgggtaaacc cggcaggcat tcagcgctgg catccagatg
     7081 aatgagactg acgccataac gccggatgag tgtgacgacc agacgacgga acgtttttga
     7141 cagacccagg cgtttaagcc gcagaaccgg gtacgaccac gcatcggtac gcaacagata
     7201 gccgttaccg gtaaaatgaa tccattctga ttcaccgaag tcactggtct ggtgtgacag
     7261 cgagtacagc caggcgttgt ccgtttccgt gatatgtgcg gtactgcagc gtatgccggt
     7321 aagggtagca aatggtggcg tgcattctgc tgaggtctgg tcagattcat caacaacacg
     7381 aagggagtag ccgttatcgc agaccatgtt aatcagttca gcgagctcaa taccatcgac
     7441 gtcaacgaca atgcgcccca tgttcagtgc ctgtacgtta acgctgtcgg cttccagtgt
     7501 cagggccagt ttcatcattt cgcctccgga tacttaccca gggtaatgtt atttaccgtt
     7561 ctgtaattgt cgcgggtcat caggccggtc gcccggcgag cccggaggat atcaatactg
     7621 tttattaact gagagcgggg acaggtgctg aatcccggct ggtcggtacg caccagcgcg
     7681 tatttttcca cgagaaaatt aactgcatca cacagtgaaa tgcctgcctc aatatgctgc
     7741 tcaatcacac gttcatcggc aaacggtgtg tcattcagtg tgaggccata atgctggtcc
     7801 agcagtcgga tgagcagtgt ctgccagatg gtgacgggag acgggcagcg agatacctcc
     7861 cgtacatgtg tgtcggataa tgttttcatc ttaagttctc tgtaactggt agtgctaaag
     7921 gtgagagctt cggggttatg aggtggttgc gggtgctggt gtcggataaa cagccagata
     7981 aacgtaacca caggagccga gggtgtcggc ttcgcaggtc agcccttttg cattcagcgt
     8041 gacggtatgc tgatatcggg gattcagttc accgctggtg agcatgagtt ccagttgttt
     8101 catcagcagc ggaaaggcct ggtccagatg gtatgcatct gcgtcgctga agcggcctct
     8161 gataccggcg cggtcggcaa ggtaatgcag gcggttaccc tcttgcacca gacgagcgcc
     8221 aaaacagggc ctcactgtgc agggaagccc ccaccagggg cggtcgtgat tgtcatcggg
     8281 atgagttatc ccggagagtt tgtctgacac gataaaatcc tctctgaaaa gtggttaaga
     8341 atggtttaac gatgacaggt cagccggcgc gctgaccggt ttcggtgaag atgtatgcat
     8401 tgatgagaat ggcctcatcc acagcggcgt ctgaggtcat ctggtcgtac tcgtttcccg
     8461 ggtgacggaa catcagttca gggtgacact catcagccgg acatacgggc caaaactgtc
     8521 cttacggcgt tctgcaaaca cggcaagaac gccgggaata tcctgcactt cacgaccagt
     8581 atacgcttca gcactgccgt gccagcgata cttaccggta cagaacggaa aaagacggga
     8641 tgcaggatgc tgttggtgaa tacgcatggc ttcaccacgg gtgatgattc tcatgatgtg
     8701 atacctctga agacagaaga taaaagtgaa aacaggtgtg atgtggttgt gacgggttaa
     8761 agcagaccat gttcggcaaa ggagaaaacc tggttgccac cgactatcag atggtccggc
     8821 acccggatat ccaccaggcc cagtgcctgt accagacgtt cggtgataag ccggtctgcc
     8881 ttactgggtg tgacttcacc ggacgggtga ttgtgcgcca gcaccacggc agcggcattg
     8941 tggtacaggg cgcgtttaat cacttcccgg ggatgaactt ccgtacggtt gatggtgccg
     9001 gtgaagaggg tttcaccggc aatcagctga ttctggttgt tcagatacag cacccggaac
     9061 tcttcccgct ccagtcccgc catgttcaga atcagccatt cccgtgccgc acgggtggag
     9121 gtgaaggcca cgccgggttc atgaagatgg cggtccaggg tttgcagggc ccgcagaatg
     9181 agactgcgct cgccgggcgt catctctccg ggcagaaagg aaagctgctg cattgtccct
     9241 gcctccattc agtcaatgat gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa
     9301 tcccgcagcc ggtaatagtg gaccgtcatg gcataacact ccgtacgaca ggcatgatga
     9361 ctgtacgtca tcagacaggc agcaataccg gcggcttccg ggctcatttc agcgcggttg
     9421 ccgttcatgg cattgaacag tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc
     9481 ataaatgccc cgccgttgtt cagggtgtac agattccaga taccaccgca gtagtcttcg
     9541 caaagacggt ccatccagcc gaagacacgg ggctccaggg tcacccactg tggaatgagg
     9601 ccaaaatgct gcggccagaa gctgatgcgc tgttcatcag ggacgggagt ggcaaccagc
     9661 tgaggtgggt tattccctga tgcagtggtt acggaaacag aaggtgtggt ggaattatgc
     9721 gaaacggttg tcatgaggtt attccttata aaaagtaaat gaatggaaga agccccgggg
     9781 caggaacaga tgtgagtcag gactgcactt tcagtgaaac ggcatcagcg catgctctcc
     9841 agcagcgttt ctgccatcac ccacaatgcg cgattgagct taatgtcggt atcaatgctg
     9901 tgaatggccc gggtatggat acgttttcct tttgcactgc gaccggaaat cccgcctttc
     9961 agcatattct cctggatggt ctgataagca ctccacaggt ccttaccgta atcctcccgg
    10021 cgtcgtggtg tcagaatgtc ggcggtggtg acgggctgat gttcgtcacc ataacggtaa
    10081 gtcagtgccg cctgtgccag cgcctggcgt gccggtggcg gcaggaccag cgactgcatg
    10141 gcatcacgct tctcctctat ccggtcaaaa acgcccacca cctcgtaagc cccttcaatg
    10201 actctgtcca ccacatttcc ccggtgtgga acacgcaatt ctcccagaga ctgaccgcag
    10261 accagtccat tggtacaaat ggcacgaaaa tatccgggta acatctggta gctggatgaa
    10321 ccgtcatggg agttgagcag aataatttca gggacatgct gaccggttat ctgtccggcc
    10381 cgccgcagac gcagcatgtg ttttgtgtat tcccggcggc tctggtcgcg cacgcgggtc
    10441 tggcaggcaa agaacggctg aaagccttcc tgctgcagat tttccaggac ggtgatagtg
    10501 ggaatgtacg catagtgttc actgcgagag gtatgtcggt tttccccaaa aatactcggt
    10561 acatggcgca tcagttcttc gtgtgtcagc ggacgatcac gacgtatctg gttcgcataa
    10621 ccaaaacgac tggctaatct catgatttgc tccttatgaa tgattaagcg atataaatga
    10681 aaaagccgcg tctcccggag aagacgcggc ctgatgggtg aaatgaatga ggtttattgt
    10741 ctgagaagcc cttcactggc gaaccacgta ttaagctgta ttccggcatc gccagtgcaa
    10801 ctgaccttca gcattgcgga taacccgccg gaaatatgtt cactggttgt ttttgtatct
    10861 tcccccaaaa ttaaataaat tcatcttaac catcacccag ataatatata tctgaaaata
    10921 ttttcaataa caccagacag gaaaaacacc accacaggcg gtggcgtttc tttggggcgt
    10981 ctttatttaa tccgggccat cgcgtttctc agtgccaaat ctgcctcttc cgttatatcc
    11041 aggcttttct gcaataccgc ctctgaccgg gtcagtgcct gacgtgctgc cagcggtttc
    11101 cggatatgct ccatcgccag catcgtgtct tcatagccct gctgacgcca ggcgtcggta
    11161 tgtgccgatg taaaactgag cagaccgccg ctgttgccac catcacctgc atatttcagc
    11221 cttttccggg gacggatctc aaggattgtg gtgtccggaa aagcctggcg atcccataaa
    11281 gaaccgtcac tcagatgcgt cacaatcacc atattgcatc cggcatcgac cagaggcgtc
    11341 acaggggtat ttccctgcat atttcgccat cctcccatac caccatcacc gaacattgtc
    11401 ccctgaacct cacggggacg gaacagcagg ggcagcgcgg ctgacgcaag cagagcctct
    11461 ttctgctgtc cgcggggcag gctctggata tgctgaaaaa cggcgttttt cgtggttccg
    11521 acacccagtt cagcacgaat gcagtcaata atatcctgca tgcccccttc tgtggggtac
    11581 agcgacacat acagcggtag cccgtctgcc agagcatcag tatcaagata atgatccatc
    11641 agcgctgtca ggggctcatc tgacaacaac ggctgagcca tcagattgtc aaacccgttg
    11701 attgtcgata tatggcgaag aagcgtcgta agcagtgctc ctgcacgtcc ggggatctgg
    11761 cagaggttca ttgcctggaa caattttttc agcaatgaaa agtaaaccaa tctgtttacc
    11821 gacag
//
LOCUS       DAZFDC010000130        11771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFDC010000130 DAZFDC010000000
VERSION     DAZFDC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.130"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            143..436
                     /locus_tag="ACHJ48_003882"
                     /pseudo
     CDS             143..436
                     /locus_tag="ACHJ48_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(494..925)
                     /gene="tnpA"
                     /locus_tag="ACHJ48_003883"
     CDS             complement(494..925)
                     /gene="tnpA"
                     /locus_tag="ACHJ48_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFO4709347.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            933..2141
                     /gene="insQ"
                     /locus_tag="ACHJ48_003884"
     CDS             933..2141
                     /gene="insQ"
                     /locus_tag="ACHJ48_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4709348.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTASQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANICRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            2185..2292
                     /locus_tag="ACHJ48_003885"
                     /pseudo
     CDS             2185..2292
                     /locus_tag="ACHJ48_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2276..2914)
                     /gene="ytfB"
                     /locus_tag="ACHJ48_003886"
     CDS             complement(2276..2914)
                     /gene="ytfB"
                     /locus_tag="ACHJ48_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO4709349.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNYPDQVAPVAPEPIQ
                     EGQPEEQPKTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3132..3752
                     /gene="fklB"
                     /locus_tag="ACHJ48_003887"
     CDS             3132..3752
                     /gene="fklB"
                     /locus_tag="ACHJ48_003887"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO4709350.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4061..5473
                     /gene="cycA"
                     /locus_tag="ACHJ48_003888"
                     /gene_synonym="dagA"
     CDS             4061..5473
                     /gene="cycA"
                     /locus_tag="ACHJ48_003888"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO4709351.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5518..6180)
                     /gene="ytfE"
                     /locus_tag="ACHJ48_003889"
     CDS             complement(5518..6180)
                     /gene="ytfE"
                     /locus_tag="ACHJ48_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO4709352.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHARHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHNTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6288..7253)
                     /gene="ytfF"
                     /locus_tag="ACHJ48_003890"
     CDS             complement(6288..7253)
                     /gene="ytfF"
                     /locus_tag="ACHJ48_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO4709353.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPNFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7361..8221)
                     /gene="qorB"
                     /locus_tag="ACHJ48_003891"
     CDS             complement(7361..8221)
                     /gene="qorB"
                     /locus_tag="ACHJ48_003891"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313217.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO4709354.1"
                     /translation="MIAITGATGQLGQYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            8310..8690
                     /gene="ytfH"
                     /locus_tag="ACHJ48_003892"
     CDS             8310..8690
                     /gene="ytfH"
                     /locus_tag="ACHJ48_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO4709355.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8808..10751)
                     /gene="cpdB"
                     /locus_tag="ACHJ48_003893"
     CDS             complement(8808..10751)
                     /gene="cpdB"
                     /locus_tag="ACHJ48_003893"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO4709356.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINNARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYEPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10941..11681
                     /gene="cysQ"
                     /locus_tag="ACHJ48_003894"
     CDS             10941..11681
                     /gene="cysQ"
                     /locus_tag="ACHJ48_003894"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO4709357.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGAKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2782 a         2945 c         3065 g         2979 t
ORIGIN      
        1 caaaaatcgc tcattatgga tataacgatg cacccgggga gaaaatgact tccacgcctt
       61 acaccgtaca ttcttgttgt gcggtatgta attattctac gctgtcggta tcggtgtatt
      121 cgttaatcag caggttaacc agccacaaaa agccattgag aaaattattg attttacatg
      181 ggattattat attgctaacc cctggttttt aaaaattgtg catagtgaaa atcaaagtaa
      241 aggtgtgcat tatgcaaaat cacagcgtct cttagaaatc aaccatgcgc acttgcagtt
      301 aatggaatcc ttattggatg aagggaagaa atacaatatt tttaagccag atatcgaccc
      361 attgcaggtg aatattaata ttgctgcgct tgggggatat tatttgatca accagcatac
      421 gcttggcctg gtttatccgg tgcgccgtaa aaccccgtcc ttcagggcgg ggatatacgg
      481 cgcggttttc cacctaacca ggtgtttgct gttgttcaat gtattgacgg atggcggata
      541 ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg cctttgtagt
      601 aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat acgcctttga
      661 ggctgtttac cagactggat atcgccagtt tgggaggata gttaattaac aaatggacgt
      721 gatctggttc gccatccatt tcaaccagtt cagcttcaaa atcagcacat acctttgaaa
      781 agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg tatctggtga
      841 caaagaccag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg atatcggttt
      901 cttttttcac agaccaaagt atcatttggc ctgtgaaacg actacaggca tttaaattcc
      961 agttaagacc cggtggtcaa caggagcgtg aaatgaggcg cttcgcaggc gcatgtcgtt
     1021 tcgttttcaa tcgtgctctg gcgcttcaga atgagaatca tgaggccggt aataaataca
     1081 tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact gaaacgcaat
     1141 ggcttaaaga tgccccgtca cagccattgc aacagtcact gaaagacctt gagcgggctt
     1201 acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg ggacagaatg
     1261 atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt atttttctgc
     1321 cgaaacttgg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt gtgaaaaatg
     1381 tcactgccag ccagtcctgc ggtaagtggt acatcagtat tcagactgaa aatgaagtat
     1441 caactccggt tcacccttca gcattaatgg tcggactgga tgctggcgtg gctaaactcg
     1501 ccacgctgtc agatggcaca gtctttggac ctgtaaacag ttttcagaaa aaccagaaga
     1561 cgctggcgag acttcagcga cagttaagcc gcaaggtcaa attcagcaac aactggcaga
     1621 agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatctgc agggactacc
     1681 ttcacaaagt cacaacgacc gtcagcaaaa accacgcaat gatagtcatt gaggatttga
     1741 aggtcagcaa catgtcaaag tcagcagcgg gtacggtcag ccagccgggg cgcaatgtcc
     1801 gggcaaaatc aggtttaaac cgttcgatac tggatcaggg ctggtatgaa atgcgccgcc
     1861 agcttgagta caagcagctc tggcgtggcg gtcaggtgct tgctgttccg ccagcgtaca
     1921 caagccagcg ttgcgcgtgc tgtggtcata cagcgaaaga aaatcgcctg tcacaaagta
     1981 aattcagatg ccaggcatgt ggatatacag cgaacgctga tgtaaatggc gctcgtaaca
     2041 ttttagcggc ggggcacgcc gttcttgcct gtggggagat ggtgcagtca ggccgcccgt
     2101 tgaagcagga acccaccgaa atgattcagg cgacagcctg aacgtagcag ggatccacgt
     2161 ccttcagggc ttggaggatg tcaacacatc agtatggttt caccccaggc actggaagcc
     2221 agacgtaagg tcatcaaaga gacaatcctt agctggcttt tggttgaccc ttcatctacc
     2281 gcgcacgaat aaaactgcca tccggctggc gggtgaacag gacctgttga ttattccccg
     2341 tatcaatggt taagcccgtc accacgccgc tggcgttctg gcggattttc accatctgcc
     2401 cgttttgcaa attactcagc ggcttacccg cgccttctac ctgcgccatt gcatagacat
     2461 cggtagcggg cagtccgtgg tcgcggaaca gctgcgccat cgttttaccg ggttcgacac
     2521 ggtaagagcg ccattgatta tcaattccgc tatctggctg gaatggttgc gtttgggtag
     2581 ttttcggctg ttcttctggc tggccttcct gtataggttc tggagcgacc ggagccacct
     2641 gatcaggata attttgtggg gtgactaatt gcgcccgcag ctgttcttct gtcggcggtt
     2701 gtgattgcga ctgaatatct aactgcgctt cacgcgtcac caccggcgcg ttgggcgtat
     2761 catcagatgg aagcagaaat ccgaccacca atacaatggc ggcaataatg atcccccgac
     2821 gatgcatcgg cggcagcggg tccatgaagc gaaaattgtc cggtgcgtgc cagactttct
     2881 ccagggttgg ttttagttca aagcgcccgg gcatggtttc ctcctgctcc gcgtcttgtt
     2941 cctcaatcat agcctatgaa taagctaacg ctatgatgtc cgtggtaaac ccgcctttat
     3001 tatattcata cgggatattg ctattgtttc tttttccctg ggatttgtca tcattcccgc
     3061 gacaaagttt tacccaaaga agtgtggctg atatgctgcc cgctacttta taccctaaga
     3121 aaggaaatac gatgaccacc ccaacttttg acaccatcga agcgcaagca agctacggca
     3181 ttggtttgca ggtagggcaa caactgagtg aatctggcct ggaagggctg ctgccagaag
     3241 cactggttgc aggtattgcc gatgcgctgg aaggcaaaca tccggctgtt ccggttgatg
     3301 tggtgcatcg cgcgctgcgt gaaatccacg agcgcgccga tgccgttcgt cgtcagcgtt
     3361 tccaggcgat ggctgctgaa ggtgtgaaat acctggaaga aaacgccaaa aaagaaggtg
     3421 tgaatagcac cgaatctggc ctgcaattcc gcgtgatcaa ccagggtgaa ggcgcaattc
     3481 cggcacgtac cgaccgcgtt cgtgttcatt acaccggtaa actgatcgac ggcaccgtgt
     3541 ttgacagctc cgttgctcgt ggtgaacccg ctgaatttcc ggttaatggc gtgatccctg
     3601 gctggattga agcactgact ctgatgccgg taggttctaa atgggaactg actatcccgc
     3661 aggaactggc atatggcgag cgcggcgcag gcgcatccat ccctccgttc agcaccctgg
     3721 tgtttgaagt cgaactgctg gaaatcctct aagcagcgca ttctgttccc ctcgaaccag
     3781 aggggagcag gcattcagca ataaaccctt cagtttgcca aacggcgcta ttttgtgttg
     3841 caaagacccc gtaagcgtgt atttttgtga gctgtttcgc gttatcaccg tgatatgaca
     3901 ctcactttaa acataaaatt aacattagat ctaaatctta gtattcatcc cgcgtattgt
     3961 tacctaatat cgatgagtcc cgatacagat tcgtcgtatc atagactgac taaaggccgt
     4021 agagcctgaa caacacagac aggtacagga agaaaaaaac atggtagatc aggtaaaagt
     4081 cgttgccgat gatcaggctc cggctgaaca gtcgctacgg cgcaatctca caaaccgaca
     4141 tattcagctt attgccattg gtggtgccat tggtacgggg ttgtttatgg ggtctggcaa
     4201 aacgattagc cttgccgggc cgtcgatcat tttcgtttat atgatcattg gttttatgct
     4261 ctttttcgtg atgcgggcaa tgggggaatt gctgctttcg aatctggaat acaaatcttt
     4321 tagtgacttc gcttccgatt tactcgggcc gtgggcagga tatttcaccg gctggactta
     4381 ctggttctgc tgggttgtaa ccggtatggc agacgtggtg gcgatcacgg cttatgctca
     4441 gttctggttc cccgatctct ccgactgggt cgcctcgctg gcggtgatag tgctgctgct
     4501 gacgcttaac ctcgccactg tgaaaatgtt cggtgagatg gagttctggt ttgcgatgat
     4561 caaaatcgtc gccattgtgt cgctgattgt cgtcggcctg gtcatggtgg cgatgcactt
     4621 tcagtcaccg actggtgtgg aagcgtcatt cgcgcatttg tggaatgacg gcggctggtt
     4681 cccgaaaggt ttaagtggct tctttgccgg attccagata gcggttttcg ctttcgtggg
     4741 gattgagctg gtaggtacaa cagctgcgga aaccaaagat ccagagaaat cactgccacg
     4801 cgcgattaac tccattccga tccgtatcat tatgttctac gtcttcgcgc tgattgttat
     4861 tatgtccgtg acgccgtgga gttcggtagt cccggagaaa agcccgtttg ttgaactgtt
     4921 tgtgttggta ggtttgcctg cggctgccag cgtgatcaac tttgtggtgc tgacctctgc
     4981 ggcgtcttcc gctaacagtg gcgtcttctc taccagccgt atgctgtttg gtctggcgca
     5041 ggaaggtgtg gcaccgaaag cgttcgctaa actttctaag cgcgcagtac ccgcgaaagg
     5101 gttgactttc tcgtgtatct gtctgctcgg cggcgtggtg atgttgtatg tgaatcctag
     5161 cgtgattggc gcgttcacga tgattacaac cgtttccgcg attctgttta tgttcgtctg
     5221 gacgattatc ctttgctcgt accttgtgta tcgcaaacag cgtcctcatc tacatgagaa
     5281 gtcgatctac aagatgccgc tcggcaagct gatgtgctgg gtatgtatgg cgttctttgt
     5341 gttcgtggtc gtgttgctga cactggaaga tgacactcgc caggcgctgc tggttacccc
     5401 gctgtggttt atcgcgctgg ggctgggctg gttgtttatt ggtaagaagc gggctgctga
     5461 actgcggaaa taaccgcatt atcatgctgg atggcgcaat gccatccagc ttttagatca
     5521 ctcacccgcc agcgcccttg ggaacagtac attgttttcc aggctgatgt gatccatcag
     5581 gtcatcaatc agttcattaa tgccgttata catcgctttc caggtggtgc aggcttccgg
     5641 cggcggtgtg acgttattgg tattgtgttt gatcacttcc agcagttcac ccgcttcatc
     5701 gtgctcgctt tccattacgc tgattggccc cattgcctgg ctgcccatgc cttgtttgat
     5761 catcgggaag aggatctgct cttctttcat catgtggctg gaaagctctt catgcagcat
     5821 ggtcaggtat tttgtcagcc cttttggcac gctcggtttg tcggcgtgaa cgcgctcgac
     5881 tttagtcgct tgcagaatca gctccggcag ttgctcgcgg tgacgagcgt ggtagcgcac
     5941 gatgatatgg tcgatgattt ctgccagcgg ggcgctacgc cagtctttct caatcggttg
     6001 ttcagcaagc tttgccagct cagcttcaat gacctcaaca tccagttctt tacgtgccgc
     6061 cgcacgcgcc agcgtttgct taccgccgca gcagtaatcc atatcatatt tacgaaacag
     6121 agccgaagcg cgaggaatag agagcgccag ttcacctaaa ggttggtcgc gataagccat
     6181 agctgatacc tcattcttaa taagataaga tgtattttaa atgcatcttt aaggcaaaag
     6241 ctataaccct tacgtagcag aggcttattt aactcactgc cagcgtgtca actttctgag
     6301 acagattcag ttaaaggttt ttctggtttt gctctaaccg cgatgaccac gccaatcact
     6361 aacagcgcga taccgctcag cgtcattagc ggcggcattt gctggcggag taaaaaggtg
     6421 tacagcaaac ctgccagcgt ttcgaaaaca atcagcggcc cgagaatcac tgtcggtaat
     6481 cgctggctgg cgacgttcca gcagagtgcg ccaacccatg agcaaagcac ggctatcgca
     6541 atcatcagac taataaacac cagcggacgg gggccaaagg gtagggagaa gtccggcgtt
     6601 tgcgtattca gccagtaaca agcgacgaga tagccgataa gtgaaaccgg cagtgtgacc
     6661 agcgcctgcg ctgttgccca catcatcgga tgtttgtcgg gattttcccg cagccagcgg
     6721 gcgttgcgca gggcatacca cgcccagcag accacggaaa ctaacgccag cacgatgcca
     6781 gaggtataac gtgcccagtc aaaattgggg agtccgtggt ttaactcagc aatattcaca
     6841 cacgccaggc cgatgccaat acaaatcagt gccggggcga gttttcccca cgcgagtttg
     6901 ccgtcgcgct ggctataaag cagattggcg aagacgggaa tcaccaccgg cagggtgccg
     6961 ataatcatcg tggaaacagg cgcaccagta cgttgaatgg cactggcaag gcagaagtaa
     7021 tagatgaggt tgcccatcat agtgagcatc aaggcggtaa gccagtcgcg acgcgccaac
     7081 tgacgcagac gcacgcgtcc cagccaggca atgggcagcg caattaaccc taacgccaga
     7141 taacgcccca tcgactgcaa catcgccggg tattccggca cgatcaacgg cccgacaaaa
     7201 ataagccccc acatcaaccc tgctaacagg gcgtacagca cgccgctaat cattactggc
     7261 atccattgat ctgtcagaag aaagttcagg aagcagacat gattgctccc ggcatcccgt
     7321 cattatcaga gaggatgcgg gaggatgcca ctttaattaa ctagttatta acattaaaaa
     7381 gatggcttac gctttcggct aacgttgtcg ttgggtggcc aatcaatttg ctaagtgttt
     7441 tgctgtcatc aaacagaccg cctttcgatg cgccaacgtc agaatccgcc agcatatccg
     7501 ccagtccgtc gggcagtccg acgcttttca gtgccgcggc gaaatcggct tcgctcagat
     7561 tttgataggt aacctgtttg ccgctctgtt tggtcagctc tgccgctaac tgtgtcaacg
     7621 tccaggcact atcgcccgcc agttcgtaaa ccttgccttc gtgaccggct tcgctaatca
     7681 cgcgtgccgc agctgccgca taatctgccc gcgttgctga ggcgattttg ccatcgcccg
     7741 ccgcaccgat aaatacgccg tgttccagtg ctgccggggc gctggcgagg tagttttcgg
     7801 tgtaccagcc gttgcgcagt agggtgtaaa cgatgccaga atcagccagc attttctccg
     7861 tctcgatgtg ctcatcggcg aggccgagcg gagatttatc cgcgtgaagc aggctggtat
     7921 aagcgataaa tttcacgcca gccgtctttg cggcattaat aacattacga tgctgcgggg
     7981 cacgttgacc cacttcgctg gaagagatca gcagtagttt ttccactccc tgaagtgcag
     8041 atgtcagtgc ggcttcatcg ccgtagtcag cctgacgcac ggtaatgcct tgtgctgtca
     8101 gtgcctgggc ttttgccgga ttacgaacga tagccactat ttggctggca ggaaccgttt
     8161 tcatcaagga ttcaataaca tactgaccaa gttggccagt ggcaccagta atagcgatca
     8221 tgggaagtct ccatcgtttt gtcttgtgtt taaatacgct aacacctaaa cttactttta
     8281 gtaagtacgc acaaaaaggt aagtatgaaa tgagtcaggt tagcctgtcg caacaactga
     8341 aagagggtaa tctctttgcg gaacagtgcc cgtcgcgcga ggtgttgaaa cacgtcacca
     8401 gccgttgggg ggtgttgatt ctggtggcgc tacgcgaagg tactcatcgc tttagcgacc
     8461 tgcggcgcaa aatcggcggg gtgagcgaaa agatgcttgc gcagtcgtta caggcgttag
     8521 aacaggatgg ttttcttaac cgtatcgcgt atccggtggt gccgccgcat gttgaataca
     8581 gcctcacgcc gctgggcgag caggtgagcg aaaaggttgc tgcactggcg gactggattg
     8641 agttgaattt gcccgaggtg ttggcggtgc gggatgaacg tgcggcataa ctgccggatg
     8701 cgcggcctac ggtgtatgac ttgtagggcg gataagatgc gccagcatcg catccgccaa
     8761 taaatgccgg atgcgattga tgatttatag gccgaaaaag aagtgtttta cttactcaaa
     8821 tccacctgat aaatcgcaaa cccgatatca tcggtcgcga ctttattcat cggatactgc
     8881 cctttctctt taataaacgc tgcggcttta tctgacgggg aagtttcgaa acggatatcc
     8941 agtttcttat cggcagctat cggtgctaaa cgccagttgt tatctgccgc cgggtgaatt
     9001 tcccccgcac gtttcgactc atcagcaatc cacgctgcca gcaccgagcg gttctcatcc
     9061 ggtgaagcaa aagcgatatg gctgtcgccc gtcccggcaa atttgccgcc gtaagcgcga
     9121 tagttattgg tggcaacgag gaacatagcg ttcggatcaa tcggcttgcc attaaaggtc
     9181 aggttcttaa tcctttccgc attggcatta atcatctggc actcgccgtc ataacgggcg
     9241 ggctgggtaa catcaatctg ataattcaca ccatcaataa catcaaagtt ataagtgcgg
     9301 aaaccatccc agttgatgag tgactgtggt ttcgtgctgt tgggatcaat ctggttaaac
     9361 tgtccggcgg agcactccag ccactctttc acctctttac cgctggcttt caccacaatc
     9421 agcgtattgg gatagagata aagatcggcg gcattacgga aggtcaactg gcctttttcc
     9481 acctccacat agcttgccgg gtcatttttg cgaccaccga ctttaaacgg tgcggcagct
     9541 gaaagcaccg gcagttttgc cagatccgga tcgccctgaa tgtaatgctc gacatacgct
     9601 ttttgcgcgt tgttcactac ttgcacggtc ggatcgtcct gcaccagcgc cagatagcta
     9661 tacatattgt cggcagattt accgattggc ttgctgacga actggcgtgt ggcatcgtga
     9721 tcggctttga gtgtttctac cagcttgctg tcttccgccg cgagggattt tttattggcg
     9781 atgtcgtaaa tcggccgtgc ttccgctttc gcctgcgtca cctgccattt accgctgtca
     9841 ttactgagtt gtaagtcgac caccccaaga tgatcgcccc acatgcccgg cattaccgcc
     9901 ggaacaccat tcagcgtgcc tttggtgata tcagcccctt cgatatcagc aaaatcttta
     9961 cccgggaaaa cggcgtgagc atggccaaac ataatggcgt taacgcccgg aatttcactg
    10021 aggtaataaa ctgagttttc cgccattact ttatacggat cggcagacag cccggaatgc
    10081 gccagaacga caacgacatc ggcacctttc tcgcgcattt caggctcgta tttgcgcacg
    10141 gtttcggtaa tatcattcac cgtcactttt ccggataaat tagctttatc ccagcccatg
    10201 atttgcggcg gcacgacgcc aatatagcca atcttcagcg tctgtttttt tccgtcttta
    10261 tcgaccactt cggtgtcttt aattaaatac ggtgtaaaca ttggctgttt ggttctggcg
    10321 tcaatgacgt tggcatttac ataagggaat ttcgctcctg ccagcgcatt tttcaggtaa
    10381 tccagaccgt agttaaactc gtggttgcca agcgttccga cggtatagtc cagcgtattt
    10441 aatgccttat agaccgggtg aatatcacct gcttttaatc ctttcgccga catgtaatcg
    10501 gccagcggac tcccctgaat caaatcgccg ttatcgacca gtacgctgtt tttcacttca
    10561 ttgcgggcat tgttaatcag gcttgccgta cgtaccagtc cgaatttttc cgtggcggtg
    10621 tctttgtaat aatcgaaatc catcatgttg ctatgcagat cagtggtttc catgatacgc
    10681 agatcgaccg tcgctgcatt cacactggcg gcaatcagcg tggccaggag cgttgcgcta
    10741 aacttaatca tcagggacat ccttttatca tcgggaatac gaaagaaaag ggagaataaa
    10801 cgtcttactt atagaacagt gaagaatgcc acaattttac gctttgaaaa tgatgacact
    10861 atcacagttg gcgcattcat taatgatagg gtataagtaa aacaataagt taacaccgct
    10921 cacagagacg aggtggagaa atgttagatc aagtatgcca gcttgcacgg aatgcaggcg
    10981 atgccattat gcaggtctac gacggggcga aaccgatgga cgtcgtcagc aaagcggaca
    11041 attctccggt aacggcagcg gatattgccg ctcacaccgt tatcatggac ggtttacgta
    11101 cgctgacacc ggatattccg gtcctttctg aagaagatcc tcccggttgg gaagtccgtc
    11161 agcactggca gcgttactgg ctggtagacc cgctggatgg tacgaaagag tttattaaac
    11221 gtaatggcga attcaccgtt aacattgcgc tcattgacca tggcaaaccg attttaggtg
    11281 tggtgtatgc gccggtaatg aacgtaatgt acagcgcggc agaaggcaag gcctggaaag
    11341 aagagtgcgg cgtgcgcaag cagattcagg tccgcgatgc gcgcccgccg ctggtggtga
    11401 tcagccgctc tcatgcggat gcggagctga aagagtatct gcaacagctt ggcgaacatc
    11461 agaccacgtc catcggctct tcgctgaagt tctgcctggt ggcggaagga caggcgcagc
    11521 tgtacccgcg tttcggacca acgaatattt gggacaccgc cgctggacat gctgtggctg
    11581 cagctgccgg agcgcacgtt cacgactggc agggtaaacc gctggattac actccgcgcg
    11641 agtcgttcct gaatccgggg ttcagagtgt ctatttacta aattcagatg gcagaaacag
    11701 tgtatttcct gattctgcca tcctgatttc tcccaaccta aaaagttata aataaaaaga
    11761 gattgtattt a
//
LOCUS       DAZFDC010000131        11677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFDC010000131 DAZFDC010000000
VERSION     DAZFDC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.131"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..4141
                     /gene="ehaA"
                     /locus_tag="ACHJ48_003895"
     CDS             158..4141
                     /gene="ehaA"
                     /locus_tag="ACHJ48_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO4709358.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSETFFMGVYGSYGGNQYLSVNNGGEVNARKQISLGY
                     YDQVSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYALGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     RWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4283..5371
                     /locus_tag="ACHJ48_003896"
     CDS             4283..5371
                     /locus_tag="ACHJ48_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4709359.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5413..6344)
                     /locus_tag="ACHJ48_003897"
                     /pseudo
     CDS             complement(5413..6344)
                     /locus_tag="ACHJ48_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(6436..6943)
                     /locus_tag="ACHJ48_003898"
                     /pseudo
     CDS             complement(6436..6943)
                     /locus_tag="ACHJ48_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
     gene            7201..7806
                     /locus_tag="ACHJ48_003899"
     CDS             7201..7806
                     /locus_tag="ACHJ48_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO4709360.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7846..8709
                     /locus_tag="ACHJ48_003900"
     CDS             7846..8709
                     /locus_tag="ACHJ48_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO4709361.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8699..10246
                     /gene="fdrA"
                     /locus_tag="ACHJ48_003901"
     CDS             8699..10246
                     /gene="fdrA"
                     /locus_tag="ACHJ48_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO4709362.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQEHLDLHTLNQPLITDVRA
                     RLKPQQKYIRGLFCGGTLCDETMFAVMEKHSDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10246..11664
                     /locus_tag="ACHJ48_003902"
     CDS             10246..11664
                     /locus_tag="ACHJ48_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO4709363.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQFDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAVDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
BASE COUNT         3080 a         2723 c         3160 g         2714 t
ORIGIN      
        1 tttattaccc tctgaactac atactcagat atgtggttgc caatgctatg caaaattttt
       61 gaatttttat atgcgatgaa cagtaaccgg aatggcattt aatgcgttgc tgtttatgca
      121 atcttggttt tatttggatg tattgctgga gattgtgatg aataaaatat atcggctaaa
      181 gtggaacagg tcccgtaact gttggagcgt ctgctcggag ctggggagca gagtaaaagg
      241 aaaaaagtcc cgggctgttt taattagcgc gataagttta tattcatctc tggtattcgc
      301 cgatgatgtc atcgtaaacc aggataaaac tattgatttt ggcaaagaga accagagcat
      361 cgattaccgt attacggtga cagacaatgc caatctggta atcaatgcga cagatacttc
      421 ccgtccgcgt ctgactctcg cttctggtgg tgggttggat attaccggag gaaaggtaac
      481 aatcaatggc ccacttaact ttttgctgaa aggtacgggg ttcctgaatg tctccaatgc
      541 tggcagcgag ttatatgctg atgatttgta tgaatcaaac tcaggcatga gacacgatcg
      601 cggctatttt aatgtctcca acggcggcaa aatccatgtt aagggcacca gccgtctgac
      661 ctatttgcag ggaaatgtca gtggtgaagg tagccaggta aattccgaaa ccttctttat
      721 gggcgtttac ggcagttacg gtggtaatca gtacctgtca gttaataacg gcggtgaagt
      781 taatgccagg aagcaaatta gcctgggcta ttatgatcaa gtctccgata caacacttgc
      841 tgtttcggaa ggtggtaaaa tttctgcgcc tactattagt ttaagcacca actctgagtt
      901 agcgttaggg gcacaggaag gaagcgcagc gaaggcagca gggattattg atgccgaaaa
      961 aattgagttt gtgtgggcaa agacatccga gaagaaaatc accttaaacc acacggataa
     1021 agacgcgact atttccgcgg atattgtcag tggcagcgag ggcctgggct atatcaatgc
     1081 gctcaatggc acgacttact taaccggtga taactctgcc tttagtggta aagtcaaaat
     1141 tgagcaaaat ggcgctttag ggatcaccca aaatataggt acagcagaga tcaacaaccg
     1201 cgggaaatta cacctgaagg ctgacgatag catgaccttt gccaataaga tctctggcaa
     1261 cggtacaata agtatcgaca gtgggacggt ggagttgacc ggcaataact atgcattcag
     1321 cggatatatt gatgttgctt ctggtgctgt cgctgttatt tctgaagaca agaatatcgg
     1381 tcgtgcagag ctggatgtcg atggcaaatt gcaaattaat gccaacaaag attgggtatt
     1441 tgataacgat cttgaaggta gaggcattgt tgaaataaac atggggaatc acgaattctc
     1501 cttcgatgag tttgcttata cagactggtt ccagggttca ctggcgttcc agaacacgac
     1561 atttaatctg gaaaagaatg ctgagtttct gcagaaaggc gggatcactg cgggtcaggg
     1621 aagcctggta acagtgggta agggcgctca ctccattagc actttgggat tctccggcgg
     1681 aaccgttgat tttggtgccc tgacagcagg tgcacagatg acagaaggga cggtcaacgt
     1741 tagtaaaacg ctggatttgc gcggcgaggg tgtgattcag gtttctgaca gtgacgttgt
     1801 ccgctcagta tctcgtgata ttgactctgc gttatcgctc actgaagtcg atgatggtaa
     1861 cagcaccatt aagttggttg atgcgcaagg tgcggaagtt ctgggcgatg cgggcaatct
     1921 gcaattgcag gataaaaatg ggcaaatcct ctccagcagc gcccaacgtg atattcagca
     1981 gaatgggcaa aaagcggccg tcggcactta cgactatcgt ctgacgagtg gggtaaacaa
     2041 tgacggtctg tatattggtt acggcctgac ccagcttgat ttacacgcta ccgacagcga
     2101 tgctctggtg ctgagctcta acggtaaaag cgagaatgcc gccgatctca gcgcaaagat
     2161 taccggcagt ggtgacctgg cattcagcag ccagaagggt cagaccgtat cgctttctaa
     2221 caaagacaac gactataccg gtgttaccga tctgcgcagt gggacgcttt tgttgaataa
     2281 cgataacgtg ttgggtaata cccatgaact gcgtctggcg gcagagactg aactggacat
     2341 gaatggtcac agccagaccg tgggcacgct caatggcagc gccgattcac tgctgagctt
     2401 aaatggcggc agtctgacgg ttaccaacgg gggcacttca accggttcgt taacggggag
     2461 cggagagctg aatattcagg gcggcacgct ggacatcgcg ggcgataaca gcaacctgac
     2521 ggcgaatgtg aacattgcta attcggctaa tgtcctggta agtcatgcgc agggattggg
     2581 tagcgcaaac gttgagaaca acggtaccct ggcgttgaat aatagcgctg aaaaaagagc
     2641 ggctgcgtct gtgaattacg ccctgggcgg caatctgacc aacaacggta cgctgatgac
     2701 cggaatgtca ggacagcaag ctggcaatgt gttagtggtg aaggggaact accacggtaa
     2761 taacggtcaa ctagtaatga atacggtact gaatggcgat gactcagtaa ccgataaatt
     2821 ggttgtcgag ggcgatacta gcggcacgac tgccgttacg gtgaataacg ctggcggtac
     2881 aggtgcgaaa acccttaacg gtatcgaact tatccatgta gacggtaagt ctgagggcga
     2941 atttgttcag gctgggcgta tcgttgcggg ggcgtatgac tacactctcg cgcgtggaca
     3001 aggggcaaat agtggtaact ggtatctgac cagcggcagt gattctcctg aactgcagcc
     3061 ggagccagac ccgatgccga atccagagcc aaacccgaat ccagagccga accctaaccc
     3121 gacacctacg ccgggtccgg atctgaatgt ggataatgac ctgcgaccgg aggcgggtag
     3181 ctacattgcg aaccttgcag cagcgaatac catgttcacc acgcgtctgc atgagcgtct
     3241 gggtaatacg tactataccg acatggtgac gggtgagcag aaacaaacca ctatgtggat
     3301 gcgccatgaa ggtggtcata ataaatggcg tgatggcagc ggccagctga aaacccaaag
     3361 caatcgctat gttctgcaac tgggaggcga tgtcgcgcag tggagccaaa acggcagcga
     3421 ccgctggcat gttggggtca tggcgggata tggcaacagc gacagcaaaa ccatttcctc
     3481 gcgaaccggt tatcgtgcaa aagcgagtgt gaacggatat agcacaggcc tctatgccac
     3541 ctggtatgcc gatgacgagt cgcgtaatgg cgcgtatctc gacagttggg cgcagtacag
     3601 ctggtttgat aacacagtga aaggggatga cttacaaagt gaatcctata aatcaaaagg
     3661 atttaccgct tcactggaag ctggatacaa acacaaatta gctgaattta atggcagcca
     3721 gggaacgcgt aatgaatggt atgttcagcc gcaagcacag gttacctgga tgggagtcaa
     3781 agccgataag caccgcgaaa gcaacggaac cctcgttcat agcaacggtg atggcaatgt
     3841 tcaaacccga cttggcgtaa aaacctggct gaagagccac cataaaatgg atgacggtaa
     3901 atcccgcgag ttccagccgt ttgtagaagt gaactggcta cataacagta aggatttcag
     3961 caccagtatg gatggcgtgt ctgtcactca ggatggagcc cgaaatattg ctgagataaa
     4021 aaccggggtg gaaggacagc taaatgccaa cctgaatgtc tgggggaatg tgggcgttca
     4081 ggttgccgat aggggatata atgacacctc tgcaatggtt ggcattaagt ggcaattctg
     4141 atctgaataa tgagtaaaag gagagagagc cgaacgggag ataattcggc tctctgtgtc
     4201 attttcttgc agccagcttt atccgtcaga gtgaagactc gcagatttag ctggcgcaca
     4261 agatttaatc aaggtgtgtt caatggcttc gcatgatttg agtgtttttt tggaagagtt
     4321 cggggcaacg gtgaatttga cgttgcctgg tatcgtgtcc gaaaaagaac ggcttttact
     4381 caagctgcta atggagggaa tgtcggttac agaaatctca cagtacagaa atcgcagtgc
     4441 aaagacaatt tcacatcaaa agaagcagct gtatgaaaaa ctaggcatcc agagtgatat
     4501 taccttctgg cgggatatct tttttcaata tcatccgcaa gtcattagtg gcacgggaaa
     4561 taaaaataat ttttatatcc ctgataaccg ttgtcaccat atcgtcacgc ctgaagccat
     4621 cagtctggcg ttggaaaacc atgaatttaa accgtggatc caaccggttt tctgcgcgca
     4681 gactggggta ctgacgggct gtgaagtgct tgtccgctgg gaacatccac aaacgggaat
     4741 catcccgccg gatcagttta ttcctctggc ggagtcatcc ggtcttattg tcataatgac
     4801 ccgccaactg atgaaacaga ctgcggatat tctgatgccg gtaaaacatt tgctgccgga
     4861 taatttccat attggtatca acgtctcggc gggttgtttt ttggccgcgg gatttgaaaa
     4921 agaatgcctg aacctggtca agaaattagg aaacgataaa atcaaactgg ttctcgagct
     4981 aacggaacgt aaccctattc cggtaacgcc agaagccaga gcgatatttg acagccttca
     5041 tcagcataac attacctttg cgctggatga ctttggtacg ggttatgcga cctatcgtta
     5101 tctgcaggcg tttccggtcg attttattaa gatcgataag tcatttgtgc aaatggcggg
     5161 tgtcgacgaa atatccggtc atattgtgga caatattgtc gaactggcgc gtaagcctgg
     5221 tctgagtatc gtggcggaag gggtagaaac ccaggagcag gcggatttaa tgatcggtaa
     5281 aggcgttcac tttttgcagg gctatttgta ctctccgcca gtaccgggta ataaatttat
     5341 ctctgaatgg gtaatgaaag caggtggttg atgtaaaccg ctattcacag cgcatcggga
     5401 ggttggcagc gattaattct cccgatgcag taaatcctga tagatccccg ttaatatgcc
     5461 atttggcgca aattgttttt taatccactg cgtcacctga cccgtagcag aatgctgagt
     5521 cgccagtaac atgcgtgaat cctggcgcgg gttatggatt tgtcgggtaa ccagcaggga
     5581 ttgcgccatc gcctcacgga ccatgtaatc tggcaaaaaa acaatccctt cacctaaaat
     5641 ctggcactga catttggtgt tgaaatctgg caccagaatt gactcctgcc cgtgcagcaa
     5701 ccagcccact tttttattaa tcgtatgcgc ggtgtcctcc accataatat tagggtagag
     5761 acgcagttgg ctttcggcga tggttccggc acaaaggcta acgggtgatc cggggcgata
     5821 gcaaaagccc agcgaatcgc gccaatttca gtgtaatcaa taccgccgcc gtccagcagc
     5881 gtgtcgggtg cgccgatagc gatatttgcc tgattattaa taatcgcatc ccagacgccg
     5941 ttatacactt cggtggtgac ggtaatctgg caggtaggaa actgcttttt cagcacctgt
     6001 aataagcgtg cggtgtgttt gggcgtataa agcaactgat taatacaaat gcgcacacgc
     6061 gcttctatgc cctgcgaaat agtatcgatg ctgcgtttga tggcgtgaaa atcattcagc
     6121 aggtcggtag ctttgcggaa aaaataacgc ccggactccg ttaactcaat gctgcgggta
     6181 ctgcgagtga acagcaccac atccagccct gtttccattc gcttgatggt gtagctaatg
     6241 gcggaagtgg ttaaacccaa ctcttctgcg gctttactga aactgccaaa acgcgcagcg
     6301 gtagtaaatg ccaacagatt ctcttcggta aaaattgagt tcattaaacc attcccgcca
     6361 ttatctaata tgaatttctc caagcataac tgtagaggta tcaattagag atgggtaatc
     6421 atttttgaat aacttttaat acccgtcgcg tttagtcgtc gcttcgctta atttttgtcc
     6481 gccatattct gaaattatcc ccacgcagtt acccgccaga aaacccagta acgtcaccgt
     6541 ccagttcatc ccgctggcaa aaaacagcgt catgccgaga aagccaccgg gaataaatga
     6601 cagtaaccag aaacgccctt gccagaccac caccgccgag aagggtacgg tagtaattac
     6661 gctcgcccag aacgcggaca gtccacttac agaggccagc caaccggcag ccagcgcggc
     6721 gaaaaacgcc cacaccatcc cggaataatt aaccgacaga cttctgataa accccgattt
     6781 accgccgcct gcggcataaa aactgcaaaa ggcgacgaag ccaatggttc ctaataattc
     6841 ctaataattc ccatccctga gagagcccga catgtgaaga cactaactgc caaagccctg
     6901 cacaaatacc gacggtgaca cccgttgccg tgagtccgtt catcttgttc ccctctggtt
     6961 agacatggac gaaagctact cttacatgtg cagactatag agaaataaaa cctgattgag
     7021 tgaaagggta tcgggtcaaa gaaacaaata ttgcactacc gcacactgcg aaaagattgt
     7081 tgaacatcat tcaacaaaaa ggcgaaatac ggcgaattaa atttaagcgg gttcttttat
     7141 cagattatcg ttgctattct caattcatcg aaaacagcca caaaaaccag gggaatgtga
     7201 atgagtataa aaaatctacc tgccgactat ttattggctg cacaacaggg tgatatcgat
     7261 aaagtaaaaa cctgccttgc gctgggtgtc gatattaata cctgcgatcg tcaggggaaa
     7321 acggcaatta cgctggcaag tttatatcag caatatgctt gcgttcaggc attaattgat
     7381 gccggagcgg atattaataa gcaagatcat acctgtttaa atcctttttt aattagctgt
     7441 ctgaacggtg atttaacgct gctacgaatt attttaccgg ctaaaccaga ccttaattgc
     7501 gtcacccgtt ttggcggtgt cggcctgacg cctgcctgtg aaaaaggcca tttaagtatt
     7561 gtaaaagagc ttttggcgca tacggaaatt aacgttaacc agaccaacca tgttggctgg
     7621 acgccactac tggaagcgat tgtgcttaac gatggcggga ttaaacagca ggcgattgtg
     7681 cagttattgc tggaacacgg tgccagcccg catctgaccg ataaatatgg caaaacgcca
     7741 ctggaactgg cgcgggaacg gggctttgaa gagattgcgc agttactgat tgccgcaggt
     7801 gcataaaccg ggaggcttgc tatcaacaca ccagaaaaac ggtgtgtgtg ggcgctaact
     7861 gcggatgcgg attttctggc gcagcgaggg caaggacagg ttgaacaggt ctttgccaga
     7921 gcggtaaata tcgcactccc ggctcgccag cagttgctga cgctgctttg tgaagagtac
     7981 gacaatgcgc caaacagttg tcggttggca ctcactcact ttgatgatct gttccggcat
     8041 ggtgataagg ttcagtttga cgatcaaggt attacggttg gtcaacatct tcatatagag
     8101 atgagtcgtt gtcggcgttg gctgtcccca accttgcaaa tgaccgctgt gaattttcgc
     8161 cttatcgcct ggcaacagtg gcacgacatc attcatcagc acctggggga aaatgaaacc
     8221 ctgtttaatt atcgcggcga taatccgttt tatcaggcgt taaataaaga attacatatt
     8281 aaacgacggg cagttataca ggcagtaatc gataaacaaa atatcgcctc agcggtcgcc
     8341 agtatgatgg gcttagggat tggccttacg ccatcagccg atgattattt aacaggtctg
     8401 gcacttattt tatttatttc cgggcatccg gcgggaaaat acaaagagga attttatctc
     8461 ggcttgcaac gcggcagaaa taataccacg ttattaagtg ccataacgct ggaagccgca
     8521 ttacaacaac gctgccggga aaatattcat cgttttattc acaacattat ttatgacatc
     8581 cctgggaacg caactcaggc aatagaaaaa attaaacata ttggctccag ttccggctgc
     8641 gacatgctgt atggcatggc cgatggttgt gcgctgagcc aaacctacgg agggaattat
     8701 gtcagttaaa atagtcatta aaccgaatac ctattttgat tctgtctcgc tgatgtctat
     8761 ctccacgcgt gcaaataaac tcgacggcgt cgagcaggca tttgtggcga tggcgaccga
     8821 aatgaacaaa ggtgtgctga aaaatttagg actgctgacg ccggagctgg agcaggcgaa
     8881 aaacggcgac ctgatgattg tcatcaatgg taaatcgggg gcggacaacg agcagttgct
     8941 ggtggagatt gaagaactgt tcaacaccaa agcgcaaagc ggctcgcacg aggcgcgtta
     9001 cgccactatt gccagcgcca aaaagcatat cccggaaagt aacctggcgg tgatttcggt
     9061 caacggtctg tttgccgctc gcgaagcgcg tcaggcgctg caaaacgatc tcaacgtgat
     9121 gctgttttcc gataacgtct cagttgaaga tgaactggcg ctcaagcaac tggcccacga
     9181 aaaagggctg ctgatgatgg ggccagactg cggcacggcg attatcaacg gtgcggcgct
     9241 ctgctttggt aacgccgtgc gtcgcggcaa catcggtatt gttggcgcat ccggcaccgg
     9301 cagtcaggag ttgagcgtcc gcattcatga atttggcggc ggcgtttcgc aactgattgg
     9361 caccggcggg cgcgacctga gcgagaaaat cggcggcctg atgatgctcg acgccatcgg
     9421 gatgctggaa aacgatccgc aaactgaaat cattgcgctt atctccaaac cgcctgcgcc
     9481 tgcggtggcc cgcaaagtgc tggaacgtgc gcgcgcctgc cgcaagccgg tggtcgtttg
     9541 cttcctcggt cgtggcgaaa cgccagtgga tgagcagggg ctacagtttg cccgcggcac
     9601 caaagaggca gcgctaaaag cggtgatgct ctccggcgtg aaacaagaac atctcgacct
     9661 gcataccctg aaccagccgt tgattactga cgtgcgcgct cgcctgaagc cgcagcagaa
     9721 atacattcgt ggcctgttct gcggtggcac gctgtgcgat gaaacgatgt tcgcggtgat
     9781 ggaaaaacac agcgatgtct acagcaacat ccagcccgat ccggaattcc gcctgaagga
     9841 tatcaaccgc agcatcaaac acaccttcct cgactttggc gatgacgact tcaccaacgg
     9901 caagccgcac ccgatgattg accctaccaa ccgcatcagc cgcttgatcg aagaggcgcg
     9961 cgatccagaa gtggcggtga tcgtgatgga ttttgtgctc ggatttggat cgcatgaaga
    10021 tccggtcggt tccaccatcg aggcgatcaa agaagcgaaa gcgatcgccg ctgccgaagg
    10081 acgcgagttg atcattctcg cctatgtgct gggtaccgat cttgatacgc catcgttaga
    10141 acaacaaagc cagatgctgc ttgatgccgg cgtgattctg gcgagcagca gcaccaatac
    10201 cggattgctg gcgcgtgaat ttatctgcaa aggggaggaa gcctgatgag ccagtcactg
    10261 tttagccaac cattgaacgt tattaacgtc ggcatcgcca tgtttagcga tgacctgaaa
    10321 aagcagcatg tagaagtgac tcaattcgac tggacgccgc cggggcaggg caatatgcag
    10381 gtggtgcagg cgctggataa cattgccgat tcgccgctgg cagacaaaat cgccgccgct
    10441 aaccagcagg cgctggagcg cattatccag tcgcatccgg tgctgattgg ttttgatcag
    10501 gcgattaacg tggtgccggg catgacgccg aaaaccattc ttcacgccgg gccgccgatc
    10561 acctgggaaa aaatgtgcgg cgcgatgaaa ggcgcggtca ccggagcgct ggtgttcgaa
    10621 ggactggcga aagatctcga cgaggcggct gaactggcgg cttccgggga gatcaccttc
    10681 tcgccgtgtc acgagcacga ctgcgtggga tcgatggcgg gtgtgacctc ggcctcgatg
    10741 tttatgcaca tcgtaaaaaa caaaacctac ggcaacatcg cttataccaa catgagcgag
    10801 cagatggcga agattttgcg tatgggcgct aacgaccaga gcgtgattga ccgcctgaac
    10861 tggatgcgtg atgtgcaggg accaatactg cgcgacgcga tgaaaattat cggcgaaatc
    10921 gatctgcgct taatgctggc gcaggcgctg catatgggcg atgagtgcca taaccgcaat
    10981 aacgccggga cgacgctgct gattcaggcg ctgacgccgg ggattatcca ggcgggctat
    11041 tccgtcgcgc aacagcgcga agtgtttgag tttgttgcca gtagcgatta cttctccggc
    11101 ccgacgtgga tggcgatgtg taaagcggcg atggatgcgg cgcacggcat cgaatacagc
    11161 accgtcgtta ccaccatggc gcgtaacggc gtcgagttcg gcctgcgggt gagcggcctg
    11221 ccggggcagt ggtttaccgg cccagcgcag caggtcatcg gcccgatgtt tgctggttat
    11281 aagccggaag attcggggct ggatatcggc gacagcgcca tcacagaaac ctacggtatt
    11341 ggcgggtttg ccatggcgac cgcgcctgca atcgtcgcgc tggtgggggg cacggtggaa
    11401 gaggctatcg atttctcccg tcagatgcgc gaaatcaccc ttggcgaaaa ccccaacgtc
    11461 accattccgc tgctcggttt tatgggcgtg ccgtcggcag tcgacatcac ccgcgtgggt
    11521 agcagcggca ttctgccggt gatcaacacc gccatcgccc ataaagatgc gggcgtcggc
    11581 atgattggcg cgggcattgt gcatccacca ttcgcctgct tcgagaaagc cattcttggc
    11641 tggtgcgaac gttacggcgt ttgacctacc tgttcat
//
LOCUS       DAZFDC010000132        11598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFDC010000132 DAZFDC010000000
VERSION     DAZFDC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.132"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..141)
                     /locus_tag="ACHJ48_003903"
     CDS             complement(<1..141)
                     /locus_tag="ACHJ48_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709364.1"
                     /translation="MTRSAYCASFCVSFYWTQANDAGDTIPARPTYNSKIRSSAFTYN
                     TIY"
     gene            216..287
                     /locus_tag="ACHJ48_003904"
                     /pseudo
     CDS             216..287
                     /locus_tag="ACHJ48_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(298..546)
                     /locus_tag="ACHJ48_003905"
     CDS             complement(298..546)
                     /locus_tag="ACHJ48_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4709365.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(774..839)
                     /locus_tag="ACHJ48_003906"
                     /pseudo
     CDS             complement(774..839)
                     /locus_tag="ACHJ48_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1061..2746
                     /gene="btsS"
                     /locus_tag="ACHJ48_003907"
     CDS             1061..2746
                     /gene="btsS"
                     /locus_tag="ACHJ48_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO4709366.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYLELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISVRREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            2743..3462
                     /gene="btsR"
                     /locus_tag="ACHJ48_003908"
                     /gene_synonym="yehT"
     CDS             2743..3462
                     /gene="btsR"
                     /locus_tag="ACHJ48_003908"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO4709367.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            3509..3979
                     /gene="yehS"
                     /locus_tag="ACHJ48_003909"
     CDS             3509..3979
                     /gene="yehS"
                     /locus_tag="ACHJ48_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO4709368.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(4021..4482)
                     /gene="yehR"
                     /locus_tag="ACHJ48_003910"
     CDS             complement(4021..4482)
                     /gene="yehR"
                     /locus_tag="ACHJ48_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO4709369.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(4607..6610)
                     /gene="yehQ"
                     /locus_tag="ACHJ48_003911"
     CDS             complement(4607..6610)
                     /gene="yehQ"
                     /locus_tag="ACHJ48_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO4709370.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWRMLSAPLRQPGIVALR
                     EYLRQRPPSCIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVASLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPLLQQQG"
     gene            complement(6607..7743)
                     /gene="yehP"
                     /locus_tag="ACHJ48_003912"
     CDS             complement(6607..7743)
                     /gene="yehP"
                     /locus_tag="ACHJ48_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO4709371.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSSIPLARDFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDLSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELATWLAENLQS"
     gene            complement(7736..10015)
                     /gene="yehM"
                     /locus_tag="ACHJ48_003913"
                     /pseudo
     CDS             complement(7736..10015)
                     /gene="yehM"
                     /locus_tag="ACHJ48_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(10026..11114)
                     /gene="yehL"
                     /locus_tag="ACHJ48_003914"
     CDS             complement(10026..11114)
                     /gene="yehL"
                     /locus_tag="ACHJ48_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO4709372.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         2764 a         2949 c         3055 g         2830 t
ORIGIN      
        1 gtagattgta ttgtatgtaa aggcagagct ccgaatttta ctgttgtaag tgggcctggc
       61 gggaatggta tcgccagcat cattggcttg ggtccagtaa aaagagacgc agaaagatgc
      121 acaataggca ctacgcgtca tgcatggatt aggtcgctca ttatttacac agtactgcta
      181 aaaaattatt aaggaaagcg cggcacttcg tatgtaagag cgtgtcgcgg ttggctggtg
      241 aactttcgat agtgcgagta ttgaatgatt tccagccgtt accgatttac gtgtttatta
      301 gcgaacaaac cactcgtcag cagactccca ggtatctttc agcgtctcct gaacaaaagt
      361 tttagctgaa tctttatcgg cggtgcgcgt aacagaaagg ccatcgttgc tggtggcttt
      421 tacgatcacc tctacatcgt cataacgttt actgatgcgt cgggttaatt cttcctttaa
      481 cgcatccaca gcaccgtttg gcattttagt gattttttct ttagcgatac agatctcaac
      541 acgcataaca ccctcctgta actgtagtta tgtacaggtg ttatttttat ctgtatggat
      601 aaccagtgtc aattcctgat attgtttatg gggcatcaat ggggcatgta tgggacactt
      661 tttatcggcg aaattcgtcg aagttcatcg acatggtaaa cgaatcatct atccaaccct
      721 tgaaaaacgg cgctcctgga cgatcttcgt cgatttttaa aaatgttgcg tcacgcgcgt
      781 aacgtgacag ggttaatatc acaaagcaac gccacttcac caattgtgta aagcgccatc
      841 gtctcaccct tgctcgcgag gtcccggttt aactttagac gcagttttgc gaaccaggta
      901 gttttgcccg ttttttgtgc atttataggg tgattttatt tttgccaggc gattttgagt
      961 gatcgtactc acgaattctc atttttctgc aagagttcaa agaaagttaa acgcaggcaa
     1021 tgtatgttac gcgttttaaa gggatgtgtg gtttgcgggt atgtacgatt ttaatctggt
     1081 gttgctgctg cttcagcaga tgtgcgtttt tttagtcatt gcgtggttaa tgagtaaaac
     1141 gccattattc ataccgttaa tgcaggtcac ggttcgtctg ccgcataaat ttctctgcta
     1201 catcgtcttt tccatcttct gcatcatggg cacctggttt gggttgcaca ttgacgattc
     1261 tattgccaat acccgtgcga taggcgcggt aatgggcggc ttactcggcg gtccggtcgt
     1321 cggtgggctg gttggcctga ccggcgggtt acatcgatat tcgatggggg gcatgaccgc
     1381 gctaagttgc atgatctcga ccatcgttga aggattgctc ggcggcctgg tacacagcat
     1441 cctgatccgt cgcgggcgca ctgataaagt ctttaacccc attaccgctg gtgccgtcac
     1501 gttcgtcgct gaaatggtgc aaatgctgat catcctggcg atcgcccgac cttatgaaga
     1561 tgcggtgcgt ctggtgagta atattgctgc gccaatgatg gtcaccaata ccgtcggcgc
     1621 ggcgctgttt atgcgtatat tgctcgataa acgcgcgatg tttgaaaaat acacttcggc
     1681 tttttctgcc actgcgctga aagtggcagc ctcgacggaa ggcattttgc gccaggggtt
     1741 taacgaagtg aacagcatga aagtggctca ggtgctgtat ctggagctgg atattggtgc
     1801 agtcgcgatt accgatcgag agaaattgct ggcctttacc ggaattggtg acgaccacca
     1861 tttacccggc aaaccgattt cttcaactta tactttaaaa gcgattgaaa ccggtgaagt
     1921 ggtctacgct gatggcaacg aagtacctta tcgttgctct ttgcatccgc aatgcaaact
     1981 ggggtcgacg ctggtaattc cgttgcgtgg tgaaaatcag cgggtgatgg gcaccatcaa
     2041 attgtatgaa gccaaaaacc gtttatttag ttcaatcaac cgcacgctgg gcgaggggat
     2101 tgcgcaactg ctttcggcgc agatcctcgc cgggcaatat gagcggcaaa aagcgatgct
     2161 cacccagtca gagatcaaac tgcttcacgc ccaggtgaac ccccattttt tgtttaatgc
     2221 gcttaacacc attaaagcgg tgatccgtcg cgacagcgaa caggccagcc agctggtgca
     2281 gtatctttcc acttttttcc gcaaaaactt aaagcggcct tcggagtttg ttactctcgc
     2341 cgacgaaatt gaacatgtga atgcttatct gcaaattgaa aaggcgcgct tccagtcgcg
     2401 gttgcaggtc aacattgcta ttccgcaaga attatcccag cagcaattgc ccgcgtttac
     2461 cctgcaaccg atagtggaaa acgccattaa acatgggaca tcacaactgc tggatacagg
     2521 gcgagtggca atcagcgtcc gacgtgaggg gcaacatttg atgctggaga tcgaagacaa
     2581 tgccggtttg tatcaaccgg taaccaatgc cagtgggctg gggatgaatc tggtggataa
     2641 gcgtttacgt gaacggtttg gcgatgacta tgggataagc gtcgcctgtg agcctgatag
     2701 ttacacccga ataacgttac gactaccatg gagggacgag gcatgattaa agtcttaatt
     2761 gtcgatgatg aaccgttagc acgggagaac ctgcgcgtat ttttgcagga gcagagcgat
     2821 attgaaatcg ttggtgagtg ttcaaacgcc gtggaaggga tcggcgcggt gcataaactg
     2881 cgcccggatg tgctgtttct cgatatccag atgccgcgca tcagtggtct ggaaatggtg
     2941 gggatgctcg acccggaaca tcgtccgtat attgtttttc tcaccgcgtt tgacgaatac
     3001 gctattaaag cctttgaaga acatgccttt gactatctgc tgaagccaat tgatgaagcg
     3061 cgactggaga aaacgctggc gcgattgcgt caggagcgca gcaagcagga tgtttcgctg
     3121 ttaccggaaa atcaacaggc gctgaaattt atcccttgta cggggcatag tcggatttat
     3181 ttgctgcaaa tgaaagatgt ggcatttgtc agcagtcgga tgagcggtgt ctacgttacc
     3241 agccacgaag ggaaagaggg ctttaccgaa ttgacattac gtaccctgga aagtcgtaca
     3301 ccactactgc gctgccatcg tcagtatctg gttaacctcg cgcatttaca ggagattcgt
     3361 ctggaagata acggtcaggc cgagttgatt ttgcgtaatg gcttaaccgt gccggtcagc
     3421 cgccgttatc tgaaaagctt aaaagaggcg attggcctgt aaaagactgc taaaatggct
     3481 ttttgcctca tcaacacctg aaggcctcat gctaagtaac gatattctgc gcagcgtgcg
     3541 ctacattttg aaagccaata ataatgacct ggtgcgtata ctggcgctgg gtaatgtcga
     3601 agccaccgcg gaacagatcg ccgtctggct acgtaaagaa gacgaagagg gttttcagcg
     3661 ttgtccggac attgttttgt cgtcattcct caatggcctg atttatgaaa aacgcggcaa
     3721 ggatgagtct gctccggcac tggagccgga acgtcgcatt aataacaaca tcgtgctgaa
     3781 aaaattacgc atcgcgtttt cgctgaaaac cgatgacatt ctggcgatcc tcaccgaaca
     3841 gcagttccgc gtttcgatgc cggaaattac ggcgatgatg cgtgcaccgg atcataaaaa
     3901 cttccgcgaa tgcggcgatc aatttttacg ttattttctg cgtggactgg cagcgcgcca
     3961 gcatgtgaag aaaagctaag acgggtatgg cggccatgcg caaacatggc cgccgacaga
     4021 ttatttcact tctttaaaac cagcggcttt catcaccagt tccatttgcg ccatagtgat
     4081 accttttttg gcatcttcag cagaaacgtt gattcctgaa ataccctgca gggctttaaa
     4141 atccactttt tccatatcga tagtcacgtt ttcctgcgcg taggtatcgg tataggttaa
     4201 tttttcttca acacccgcga tgtttttgta tttggcgctt aacggctcaa gtgtcttggc
     4261 agcatcttct ttggtggtag caccaatgga ggcaaattga attttggttt cagaagattg
     4321 cttaagcacc ttgtcacctt tgtagacata ggtaatggca atttcagtgc cgttcagatt
     4381 ggcgctgaat ttcttcgatt cttctttgtc accgcagcca gcaagagaaa aaaccagaac
     4441 agatgcaaca acaagggaaa acagcttatt gaaagccttc atgtaaaact ccattttatt
     4501 taatcaagga actggtgact ctcaccaggg gctatatagg atatgcctaa taccgtggcg
     4561 tgagcagtcc ggaactggag tagaactctt agtaaaaagc actatttcat ccttgttgct
     4621 gaagcagggg gaataattgt tcgcacagca aaacaccgtt attcattgct tctacccgtg
     4681 cctcgctttc tgtattacga aattgtccca atacatgtgc cagccgataa aaacccaccg
     4741 cggtgaggtc attcgccagc aactctgcct gaccaatagc actctgttcc tgatagcgcc
     4801 agccgttatg gagcagttga ataagtaatg cctggcagcg catcagcaac tgatgagcag
     4861 tagacggcac aggcagaacg ctggcagaag gtagtgatgc caccacaggc gcagtttctg
     4921 cgtccagcgc ccaggcacga gtctttgtca tcattacctg tggttccagt gtcaattgcc
     4981 catcaacaaa actgacaaag ccagaaacca gacacacggg gtcgtctgtt tgttgcaaaa
     5041 gcgccgccat gcgttcaacg gcataaggcg cgctggctga ggctggcagt gataacgtca
     5101 gcagattatc ttcaccttcg ccgctgatta cctgcgcatc cagcgtctgg cggctgctat
     5161 cccaaccgag cgaaatacac tcagcgaccg gcagaataaa taagttatcg acctgattaa
     5221 gaggccgtat gcaggagggg ggacgctggc gtaaatattc ccgcaaagcc acaatgcccg
     5281 gctggcgtaa cggcgcgctc aacattcgcc aggcatcagg cgacagcggc acaacgctgc
     5341 ttaagcggtt gcgggtagct aacagcagct cgccatcggc actgcgtttt gctgcttgtg
     5401 aaacaatttg cccacccgcc agtgcgccag cctgaaaact aaacagccga cgcgtagctg
     5461 ccggtgagtt ttcctgttca cttcgcggcc aactgcgcga aaggtgcaaa atactgccgg
     5521 tgtcgggatc ggtaaaccag atgcgtaaac cataatgctc aatatcctgc cagcaacgca
     5581 tacctaaaga caccagccgc agatgatcaa gctttgcttc tccggcaatg ccagagccaa
     5641 cgaccgtgcg ccacggcaca ggaggaactt caccaacact gtcgcgccgg gccatctctt
     5701 gtgcgcaatt taatcgactg tttaatgccg caagctgacg taagcattct cctgcatgat
     5761 agtggctggc gcgggcgtgg aaggcatcaa cgctggcgcg cagttgccgt agcgattcac
     5821 tcacccagcg ccagttgcag cgttccgccg cttgctgcgc gcggctgaaa gcggcctcgt
     5881 agtgaataag cggctggctg atgccgccaa gccataatgc ctggcttaat tgctgaacat
     5941 attgacgaca cgcgttgcct tcttcgctgg caaacgggtc gtcagatgat gtgacgtgtt
     6001 cgctgcgcat ctgccagatt aaatgagtaa attccgcttg ctgagttttg gcctcgacga
     6061 aggcctgcac agccagtacg acatgttcgc aaagtgtgcc ttcaatacaa tcacagcggg
     6121 cgaagcgaat gctgctgcgg gaataaaaac gcacatcact catcggtaag cgggctgagg
     6181 gaatttcgcc aggcgcacag aacaactcaa tggtgatgcc tttagcgacc agcacctgtg
     6241 cacgtttgcg ggtggcatcg ggcagggtag ccagttcttc cagccagatt gcaggctgcc
     6301 actcttcttc tttttccgtg ggccgggtgg tggcacaaag tcgctgataa cttaacacca
     6361 gcatcacgcg atgacggcac atgccgctag ccccgcaggt gcactgagcc tctttcagtg
     6421 cctggccgtt cgccagctgg gtacggacac cgtcactgaa ggtggcgatt aaagcgccgt
     6481 tctcatggct gatttccggg acgttgccat tttccagttc cttaaggctg cgcttaacaa
     6541 aaccggcatt gcttaacgcc gtcagggcct gcggcgtgag ttctaataat tccggacgta
     6601 gtgaattcat gactgcaaat tctccgcaag ccaggtagcc agttcacccg gtgtcattgc
     6661 ggctatttgt gcgccaacat taaccagcgc ctgggccata tcgcggtcat agcaaggcgt
     6721 tgcggtgctg tcgagcgctg ccagccccag cactttgatg ccgctctgga cacacttttt
     6781 cacctgatgc gtcagcaatg atgatgaacc cccttcataa aaatcactca cgaggataat
     6841 gacgcttttt gctggttgtt caataagttg ccgcccatac tccacggcac tggcgatatt
     6901 ggtcccgccg cccaactgta ctttcattaa taactctacc ggatcggcaa cgtctgccgt
     6961 gagatcaacg acactggtgt caaacgccac cagatgggta cgaatgccgg gtaactgcca
     7021 caaacaggcc gccattaccg cagagtggat caccgaatcg accatcgatc cgcttagatc
     7081 aaccagtaag accagttgcc attgttcact gtggcgctta attcggctgt taaagcgggg
     7141 ggattcgata tacaacttgc cgtgttgcgg gtgccagtgt tgcagattgg cgcgaagagt
     7201 acttttgaaa tcaaagtctc gcgccagtgg aatagatgag cggcgacggc gatcgcggac
     7261 accagaaaaa gcctgacgaa cttcctttgc cagtcgagcc ataatttctt caacaacctg
     7321 gcgcactatc cggcgggcgg cagccagtac ttcggggttc atcagatgtt tggtgtgcaa
     7381 aacggcgcgt agcaggcttt cagaaggctg catacgttcc agcacgtcga gattcgtcac
     7441 cacatcttca atgccgtagc gcagtacggc atcgctttcc agccgctcaa tcacctgttg
     7501 cggaaacagc gtgtgaatac tgttgatcca ctcaggggtg gtgagatttg agccacctaa
     7561 tccaccggag cgttcaccac gctggagccg ttcaggatcg cgcccataca gccactccag
     7621 cgcgtggtct atctgccggg cgttgtcatc cagcccacaa agcgtcgttt ctgccgcttc
     7681 gccaagaatt aatcgccagc gttgtagctc acgggtggtc agaagatcgt tcagttcaga
     7741 catggaaaac tccccagttt tgcagcgatg ccagtgcctg ctgttcgagt tgttgatgat
     7801 gtgcaatggc ttgtggtggg caatgcaacg gcatttgcag cgccgaaacg ggaagttgcg
     7861 ccagttgata atgttcaagc acctgatgcg ccagtgtccc gcgttctcgt ggcggtagcc
     7921 aggccatcgc cgcgcgtaaa tcgggcaggg cattgataaa atcggcatca cttggttgat
     7981 ttaaatgact gctgaaaccg gcgataaatg ccggctgaca ggccagttga tggcgggcca
     8041 gcgccagcaa accgtgcagc gcttcgccgg attgtgccgg ggataactgc gccagcatcg
     8101 tcagcgccgc ttcggcacta gcgttgggat gttccagacg catcagtgcg ccaagagccg
     8161 cgccgcgatc cagcgccggg gcatggattg cctgactgcg tcgctccagt aaggctaccg
     8221 ccgcagaaag agaaacgccg ggtaaattaa cgccgctatg tagatcgcgc agaatatggc
     8281 aaagcgcttg ccagctatgc aggtgagcgt gaaactcctt ttcatccggt ctgccgttag
     8341 attcacacag ccacagcgtg cgatcgatag ccgcgcataa cgtcgtttgt gatatctgcg
     8401 caccttgcat accgctaatt tcatccagcc gccataaggc atatagcact tccagcgccg
     8461 ggcccatttc ggcaaattga ttttcctagg cgattaataa tgtgagttgc tccagtaatt
     8521 gttgactgaa ggacgccaga cccgctaacg ccgcctggct aagacatgtg gtgatactgc
     8581 cgattccacc cgcgtccagc atatcggctt ctaatttatg gcgtgcggct tcctggagtg
     8641 tggcaccaaa gcaggcggcc tcaatcaatg ccgcatgctg gctaagcggg cgggttaatt
     8701 tccagcgttc ttcaccgttg cctgcaagtg tcagtgtgct tccctgctgg cgtacaatcc
     8761 ctgggatctc cagtattgcc agccgatgta acacctgact ttgcgctagt ccattggggt
     8821 taaagcgatt taaggtaagc tccgccgaaa gagagattgc ggtacgttcc agttcgcagg
     8881 tgacatcttt tggcagaggc ggttgtggcg tagaaggggc aagtttaccg aatccgtcac
     8941 ccgctaatgt gtctatcaac gttagcagaa tcggatcggt atcgggatga ataacgccgc
     9001 gatagctcca cggcaacggc gcgttcaggg cttctttaat cagcgagcct gctatggcat
     9061 ccagccagtc agtgcgtaac ggtagtttat atccgcgcaa ctgtgccagc gccatcgcat
     9121 gcagatgagc ggcagccata tccgcggtcg aagcaggcaa gtggtgttgg cgcaaacggg
     9181 taagaaccgt ttttagcagt tgttctcccg cctgctgtaa gccccactgc cagcaccagc
     9241 tttgccagac cggggcaggc attcctgaaa ggtatcctgc cagcacatca aggcgctttt
     9301 cactgtaggg tgtgagataa caacctgtaa cggcatctgc cagcgagggc aattctggct
     9361 tgttaatgtc ctgcgggcat tcgcgccaca tttttgccag tgccggagcg tgccagccac
     9421 cgcagacgac taacacgtcg ccattatttt gctgcactgc ccatgcaatc cagcgagcca
     9481 taaaggcttc gcgctgacga ttgagtgcat cgccggggaa atcgccccgc agttgggcaa
     9541 aatagtgcgc cagtgcagag ggtaatgcag tttgctggct ttcatcttcg aacaagtgat
     9601 cccacagggt atcgctgtta tccatgcggg tggcacgcag cagcaaggcc tggctttcct
     9661 cttgcgtatc aggcgaatcg tcctcttctt cactctgcgc ccagcaaggc aaatcgataa
     9721 agtaagtttg tgcctgaata tgacgtgcgg cttgtagcgc ctgccactcc ggcgaaaatt
     9781 cggcaaatgg cgtccaggcg ccgcgcccag gagcagcacc gtcctgatac tggcaataac
     9841 tgtaaatagc caccggaagc tggtgggcta aaaacagttc atctacccgg tcattaaaat
     9901 cagccgggcc ttcaatcaac acgtatcgtg gtcgctggct ttcgattaac gatttcacca
     9961 gtcgggcgca ggccggacta tgatgccgga tgccgacaat taacggctcg ctcatgcatc
    10021 tttcctcacg gcaggcggtg gcgggcttga taataagcct gccagtgagc ttctttatgt
    10081 gtggcgacac gctgttcgaa gtaacggcgc agacgagcgc gatcttcttc gttatctttg
    10141 acgatggttc cggcaataca gtccaccagg tcggcaggct cccccgcgcg attcgctaaa
    10201 aaccaggcgc ggacgcccac agcgtgggcc acgttcacgg cttcagcggt ggacataatc
    10261 gctgttagtg tatccattga cgttttcttt tcgccgttgg cacgcagatc gcggaaggtg
    10321 cggaccagta actccagtac cgcgtcaggc actttatgcg gaataccgct atgggccagc
    10381 aaacgcgccg aagcactggc gacaagctcc agctcctggg cgaaatccat aatcggaaag
    10441 acagtttcaa aatcgaagcg acgctttagc gcagcactca tttcattgac gccgcgatcg
    10501 cgggtattgg cagtggcaat aatattaaag ccttctcgcg catagagctg actggcttca
    10561 ccagtgagtt ccggcaccgt catcaccctg tcagaaagca tacctaacag acagtcttgc
    10621 acttccagcg gtgtacgcgt tatctcttca aaacggacga ttttgccgtc gcgcattccc
    10681 tggtaaagcg gggcagggac cagcgcttcc gttgatggac catgattgat aagcagggcg
    10741 tagttccagc cgtacttgat ttgatcttcg gtagtagatg cgccgccctg gatggttaac
    10801 ccggcatcgc cgctaataga ggttgccagt aattcagaca ggagagattt tgcggtgcca
    10861 ggttccccca ccaacatcaa accgcgtccg gtggcaagag tgactaacat acgttcgaca
    10921 gcggagggac taatcaccac tttacggcta atattttgcg cgctatcgcc aaggataaaa
    10981 gcacgggccg caggcaaact taactgccaa ccgggcgggc aaggtgcgtt atcattttgt
    11041 tttaattttg ccagttcatc ggcgtataac acagcagcag gcggacgctg cagatgatta
    11101 ttctgcggtg acatatattc atccctgaaa tgtattaatg aaataaacga tattttacgc
    11161 gcaataaatg tttggcgcat tagttgtttg agcgaaaaat atctgggaag cagatcacag
    11221 taaataaaaa aacctgctgg tgaggcaggt tcttttattt actattttca ggcctaacac
    11281 cacagcctgt gcaacatcgt gttccaacga acgaattata accacggctg gaccccgttg
    11341 caattaagat aatgagattt ataatcctga ctgttgggac gttgcgccgg atcgaaccta
    11401 tttcaagtcc tggctaccgg acggggggtg ctgcaggtgg tgccctgatc cggagtgcaa
    11461 catcgtggtc caacatacgt aaaccagtat cgacaggcag tgtaagggta aggtgctggt
    11521 gttgcgctcc ttaataagga gagcggatgg actcgctgac acaaaagtta accgtgctca
    11581 ttgctgtact ggagctat
//
LOCUS       DAZFDC010000133        11584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFDC010000133 DAZFDC010000000
VERSION     DAZFDC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.133"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..216
                     /locus_tag="ACHJ48_003915"
     CDS             <1..216
                     /locus_tag="ACHJ48_003915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709373.1"
                     /translation="LCRIPEDPHHWNGFSGEKRILYGHSAHHFFFSSSTIHVSLPRLI
                     TDYEVQKYLSFQLPVIRYRERFWQYME"
     gene            223..2646
                     /gene="pgaA"
                     /locus_tag="ACHJ48_003916"
     CDS             223..2646
                     /gene="pgaA"
                     /locus_tag="ACHJ48_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO4709374.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2655..4673
                     /gene="pgaB"
                     /locus_tag="ACHJ48_003917"
     CDS             2655..4673
                     /gene="pgaB"
                     /locus_tag="ACHJ48_003917"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO4709375.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4666..5991
                     /gene="pgaC"
                     /locus_tag="ACHJ48_003918"
     CDS             4666..5991
                     /gene="pgaC"
                     /locus_tag="ACHJ48_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO4709376.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5993..6406
                     /gene="pgaD"
                     /locus_tag="ACHJ48_003919"
     CDS             5993..6406
                     /gene="pgaD"
                     /locus_tag="ACHJ48_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO4709377.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6456..7244)
                     /gene="phoH"
                     /locus_tag="ACHJ48_003920"
     CDS             complement(6456..7244)
                     /gene="phoH"
                     /locus_tag="ACHJ48_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO4709378.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7864..9135)
                     /gene="efeB"
                     /locus_tag="ACHJ48_003921"
     CDS             complement(7864..9135)
                     /gene="efeB"
                     /locus_tag="ACHJ48_003921"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO4709379.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKGIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9141..10268)
                     /gene="efeO"
                     /locus_tag="ACHJ48_003922"
     CDS             complement(9141..10268)
                     /gene="efeO"
                     /locus_tag="ACHJ48_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO4709380.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10326..11156)
                     /gene="efeU"
                     /locus_tag="ACHJ48_003923"
     CDS             complement(10326..11156)
                     /gene="efeU"
                     /locus_tag="ACHJ48_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO4709381.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         3048 a         2841 c         2720 g         2975 t
ORIGIN      
        1 ttatgccgta ttcctgaaga tcctcatcat tggaatggat tttcgggcga gaaaaggatt
       61 ttatatggac actctgctca tcatttcttc ttctcatcat caacaattca cgtctctctt
      121 ccgcgtttaa taacggatta tgaggtgcaa aaatatcttt cttttcagtt acctgtaatt
      181 agatacagag agagattttg gcaatacatg gagtaataca ggatgtattc aagtagcaga
      241 aaaaggtgcc cgaaaaccaa atgggctttg aaacttctta ctgccgcatt tttagcagcg
      301 agtcccgcgg cgaagagtgc tgttaataac gcctatgatg cattgattat tgaagctcgc
      361 aagggtaata ctcagccagc tttgtcatgg tttgcactaa aatcagcact cagcaataac
      421 caaattgctg actggttaca gattgcctta tgggccgggc aagataaaca ggttattacc
      481 gtttacaacc gctaccgtca tcagcaatta ccagcgcgtg gttatgcagc tgtcgccgtc
      541 gcttatcgta acctgcaaca atggcaaaac tcgcttacac tgtggcaaaa ggcgctctct
      601 ctggagccgc aaaataagga ttatcaacgg ggacaaattt taaccctggc agatgctggt
      661 cactatgata ctgcgctggt taaacttaag cagcttaact ctggagcacc ggacaaagcc
      721 aatttactcg cagaagccta tatctataaa ctggcggggc gtcatcagga tgaattacgg
      781 gcgatgacag agtcattacc tgaaaatgca tctacgcaac aatatcccac agaatacgtg
      841 caggcattac gtaataatca acttgctgcc gcgattgacg atgccaattt aacgccagat
      901 attcgcgctg atattcatgc cgaactggtc agactgtcgt ttatgcctac gcgcagtgaa
      961 agtgaacgtt atgccattgc cgatcgcgcc ctcgcccaat acgctgcatt agaaattctg
     1021 tggcacgata acccagaccg tactgcccag taccagcgta ttcaggttga tcatcttggc
     1081 gcgttattaa ctcgcgatcg ttataaagac gttatttctc actatcagcg attaaaaaag
     1141 acggggcaaa ttattccgcc ctgggggcaa tattgggttg catcggctta tctcaaagat
     1201 catcagccga aaaaagcaca gtcaataatg accgagctct tttatcacaa ggagaccatt
     1261 gccccggatt tatccgatga agaacttgcg gatctctttt acagccacct ggagagtgaa
     1321 aattatccgg gcgcgctaac tgtcacccaa cataccatta atacttcgcc gcctttcctt
     1381 cggttaatgg gcacgcctac gagcatcccg aatgatacct ggttacaggg gcattcgttt
     1441 ctctcaaccg tagcaaaata tagtaatgat cttcctcagg ctgaaatgac agccagagag
     1501 cttgcttata acgcaccagg aaatcaggga ctgcgcattg attacgcgag tgtgttacaa
     1561 gcccgcggtt ggcctcgtgc agcagaaaat gaattaaaaa aagcagaagt gatcgagcca
     1621 cgtaatatta atctggaggt tgaacaagcc tggacagcat taacgttaca agaatggcag
     1681 caggcagctg tcttaacgca cgatgttgtc gaacgtgaac cgcaagatcc cggcgttgta
     1741 cgattaaaac gtgcggttga tgtacataat cttgcagagc ttcgtatcgc tggctcaaca
     1801 ggaattgatg ccgaaggccc ggatagtggt aaacatgatg tcgacttaac caccatcgtt
     1861 tattcaccac cgctgaagga taactggcgc ggttttgctg gattcggtta tgccgatgga
     1921 caatttagcg aaggaaaagg gattgttcgc gactggcttg cgggtgttga gtggcggtca
     1981 cgtaatatct ggctcgaggc agagtacgct gaacgcgttt tcaatcatga gcataaaccc
     2041 ggcgcgcgcc tgtctggctg gtatgatttt aatgataact ggcgtattgg ttcgcaactg
     2101 gaacgcctct ctcaccgcgt tccattacgg gcaatgaaaa atggtgttac aggcaacagt
     2161 gctcaggctt atgttcgctg gtatcaaaat gagcggcgta agtacggtgt ctcctgggct
     2221 ttcactgatt tttccgacag taaccagcgt catgaagtct cacttgaggg tcaggaacgc
     2281 atctggtctt caccatattt gattgtcgat ttcctaccca gtctgtatta cgaacaaaat
     2341 acagaacacg ataccccata ctacaaccct ataaaaacgt tcgatattgt tccggcattt
     2401 gaggcaagcc atttgttatg gcgaagctat gaaaatagct gggagcaaat attcagcgca
     2461 ggtgttggtg cctcctggca aaaacattat ggcacggatg tcgtcaccca actcggctac
     2521 gggcaacgca ttagctggaa tgacgtgatt gatgctggcg caacgctacg ctgggaaaaa
     2581 cgaccttatg acggtgacag agaacacaac ttatacgttg aattcgatat gacattcaga
     2641 ttttaaggat aaatatgtta cgtaatggaa ataaatatct cctgatgctg gtgagtataa
     2701 ttatgctcac cgcgtgcatt agccagtcaa gaacatcatt tataccgcca caggatcgca
     2761 aatctttact cgccgagcaa ccgtggccgc ataatggttt tgtagcgatt tcatggcata
     2821 acgttgaaga cgaagctgcc gaccagcgtt ttatgtcagt gcggacatca gcactgcgtg
     2881 aacaatttgc ctggctgcgc gagaacggtt atcaaccggt cagtattgct caaattcgtg
     2941 aagcacatcg aggaggaaaa ccgctaccgg aaaaagctgt agtgctgact tttgatgacg
     3001 gctaccagag tttttatacc cgcgtcttcc caattcttca ggccttccag tggcctgctg
     3061 tatgggcccc cgtcggcagt tgggtcgata cgccagcgga taaacaagta aaatttggcg
     3121 atgagttggt cgatcgagaa tattttgcca cgtggcaaca agtgcgagaa gttgcgcgtt
     3181 cccggctcgt tgagctcgct tctcatacat ggaattctca ctacggtatt caggctaatg
     3241 ccaccggcag cttattgcct gtatatgtaa atcgtgcata ttttactgac cacgcacggt
     3301 atgaaaccgc agcagaatac cgggaaagaa ttcgtctgga tgctgtaaaa atgacggaat
     3361 acctgcgtac aaaggttgag gtaaatccac acgtttttat ttggccttat ggcgaagcga
     3421 atggcatagc gatagaggaa ttaaaaaaac tcggttatga catgttcttc acccttgaat
     3481 caggtttggc aaatgcgtcg caattggatt ccattccgcg ggtattaatc gccaataatc
     3541 cctcattaaa agagtttgcc cagcaaatta ttaccgtaca ggaaaaatca ccacaacgga
     3601 taatgcatat cgatcttgat tacgtttatg acgaaaacct ccagcaaatg gatcgcaata
     3661 ttgatgtgct aattcagcgg gtgaaagata tgcaaatatc aaccgtgtat ttgcaggcat
     3721 ttgctgatcc cgatggtgat gggctggtca aagaggtctg gtttccaaat cgtttgctac
     3781 caatgaaagc agatattttt agtcgggttg cctggcaatt acgtacccgc tcaggtgtaa
     3841 acatctatgc gtggatgccg gtattaagct gggatttaga tcccacatta acgcgagtaa
     3901 aatacttacc aacaggggag aaaaaagcac aaattcatcc tgaacaatat caccgtctct
     3961 ctcctttcga tgacagagtc agagcacaag ttggcatgtt atatgaagat cttgccggac
     4021 atgctgcttt tgatggcata ttgttccacg atgatgcttt gctttcagat tatgaagatg
     4081 ccagtgcacc ggctatcacg gcttatcagc aagcaggctt tagcgggagt ctgagcgaaa
     4141 ttcgacaaaa cccggagcaa tttaaacagt gggcccgctt taaaagtcgt gcgttaactg
     4201 acttcacttt agaacttagt gcgcgcgtaa aagccattcg cggtccacat attaaaactg
     4261 cacgaaatat ttttgcactt ccggtaatac aacctgaaag tgaagcctgg tttgcacaga
     4321 attatgctga tttcctaaaa agctatgact ggaccgctat tatggctatg ccttatctgg
     4381 aaggtgtcgc agaaaaatcg gctgaccaat ggttaataca attgaccaat caaattaaaa
     4441 acatccctca ggctaaagac aaatctattt tagaattaca ggcacaaaac tggcagaaaa
     4501 atggtcagca tcaggctatt tcttcgcaac aactcgctca ctggatgagc ctattacaac
     4561 tgaatggagt gaaaaactat ggttattatc ccgacaattt tctgcataac caacctgaaa
     4621 tagaccttat tcgtcctgag ttttcaacag cctggtatcc gaaaaatgat taatcgcatc
     4681 gtatcgtttt ttatattatg tctggtgtta tgcatacccc tatgcgtagc gtactttcac
     4741 tctggtgaac tgatgatgag gttcgttttc ttctggccgt tttttatgtc cattatgtgg
     4801 attgttggcg gcgtctattt ctgggtctat cgtgaacgcc gctggccgtg gggagaaaac
     4861 gcaccagctc cccagttgaa agataatccg tctatctcca ttatcattcc ctgttttaat
     4921 gaggagaaaa acgttgagga aaccatacac gccgctttag cacagcgtta tgagaacatt
     4981 gaagttattg ccgtaaatga cggttcaaca gataaaaccc gtgccatcct ggatcgcatg
     5041 gctgcacaaa ttccccattt gcgggtcatt catctggcgc aaaaccaggg gaaagccatt
     5101 gcgcttaaaa ccggagctgc cgcggcgaaa agtgaatatc tggtgtgcat tgatggcgat
     5161 gcgttattag accgcgatgc ggcggcatat attgtggaac cgatgttgta caacccgcgt
     5221 gtgggtgccg taaccggtaa tcctcgtatt cgaacacgtt ctaccctggt gggtaaaatt
     5281 caggttggcg agtattcctc aattattggt ttgatcaagc gaacccagcg tatctatgga
     5341 aacgtattta ccgtttccgg tgttattgcc gcatttcgtc gcagcgccct ggcagaagtg
     5401 ggttactgga gtgacgatat gatcaccgaa gatattgata ttagctggaa gctgcagttg
     5461 aatcagtgga cgatttttta cgagccacgg gcactgtgct ggatattaat gcctgaaacg
     5521 ttaaaagggc tgtggaaaca gcgcctgcgc tgggctcagg gcggtgcaga agtattcctc
     5581 aaaaatatga caaggttgtg gcgcaaagaa aactttcgaa tgtggccgct gttttttgaa
     5641 tacagcctga cgacaatatg ggccttcacc tgcctggtcg gtttcattat ttacgcagtc
     5701 caacttgccg gtgtaccgtt aaatattgaa ttgacacata tcgctgcgac acatactgcc
     5761 ggaatattat tgtgtacgtt atgtttactg caatttattg tcagcctgat gatcgagaat
     5821 cgctatgagc ataatctgac ttcatcgctt ttctggatta tttggttccc ggttattttc
     5881 tggatgctga gcctggcaac gacattggta tcatttacac gagtcatgtt gatgcctaaa
     5941 aagcaacgcg cccgttgggt aagtcccgat cgcgggattc tgagaggtta atatgaacaa
     6001 tttaattatt acgacccgac aatcaccagt acgtttactg gttgattatg ttgccacaac
     6061 catcttgtgg acattatttg cgttgttcat attcttattc gccatggatc tgctgacggg
     6121 ttattactgg caaagcgagg ccagaagccg acttcagttc tattttttgc tggcagtggc
     6181 gaatgccgtc gtgttaattg tctgggcgct gtacaataag ctgcgttttc aaaaacagca
     6241 gcatcatgca gcctaccaat atacgccgca agaatatgca gagagcttag caatacctga
     6301 tgagctctat cagcaactac aaaaaagcca caggatgagc gtacacttca ccagccaggg
     6361 gcaaataaaa atggttgttt cagaaaaagc gctagtcagg gcataaacac ccaaaacaaa
     6421 gcccggttcg cccgggctct gcaccgataa tacacttaac tgtatgcatg cagcgtacgt
     6481 tggcaaagtg ccgaacgtac gcaatcctct tttccgaacc ggacgatccc aaccatttca
     6541 tcttcttcga aacgttccag cgcgtcactt aatccggagc acacgccgcg aggcaaatcg
     6601 cattgcgtga tatcaccgtt gacgataacc gtcacgttct ccccgaggcg ggttaaaaac
     6661 attttcattt gcgcggcagt cacattctgc gcctcgtcaa gaatgacgac tgcattttca
     6721 aaggtacgtc cacgcatata ggcgaacggc gcaatttcca ccttccctat ttccggtcgc
     6781 aggcagtact gcataaagga agcccctaag cgccggacca gcacgtcgta gaccgggcga
     6841 aaatagggag caaacttttc tgcgatatct ccaggtaaga agccaagatc ttcatcggct
     6901 tgcagaactg gacgggtgac gataatcctg tcgacatcct tatgtatcag ggcctctgcc
     6961 gcttttgctg cgctgatcca ggtttttccg cacccggctt cgcccgtggc gaatatcagc
     7021 tgcttactct caatagcctt cagatagtgc aattgcgctt catttcgcgc gaggatgggc
     7081 gaagtatcgc gactgtcgcg ggccatacca atggcttcta cgccgcccat ctgcacaagc
     7141 gaggtgaccg attcttcttc acgctgctta tggctgcgcg aatcccgtct cagcacacgt
     7201 tttgcctcgc gacgagcttt gatcactgct ttttgtcttc ccatggagag caccttgagt
     7261 tgtttgtatt catcacacgc gccgttggca gcgcgattat gcgcacgaac atcagagggt
     7321 tggcttcctt gtaagccata gtttgctttt ggataaaatg ccgaaaaacg gctacgcgca
     7381 ccgtttacgg cgtcggtaac acatgaaaag aaaggatgag gttgaaaatg caaagtgacg
     7441 agatgactac cggaggagaa aactccgcga gtggtggcgc gttgattatc taaaacatgt
     7501 ccagtacagg acgttaccat ccgcgatctc catagtgact gactatcact gccgggaact
     7561 tccgctgcta cttaataagt acaacagttc tcgcatttat tgcaacaata tatttactta
     7621 tatttaacta taaaacacca tttcagtgac attagtttct actggaaaga tgacagagtg
     7681 atgacagata tgaaaaaagc tgtgtgcttt cagcaggatt tgcatcaggc atcagtgcac
     7741 gatcgccgta tgcggcgtga acgccttatc cgcctaccga acccgcaccg attgtaggca
     7801 taagaagacg cgcaagcatc gcatcaggca tctgcgcaag aacgccttat ccgtctaaaa
     7861 acattaaacc cgcaataacg cgcttccgag ataatcgttc gcgtccttca ctcccggcag
     7921 cgcaaaaaaa taaccgccgc cgataggttt aacgtattcc tccagcgctt cgccattgag
     7981 ccttttttgt actgtcagga agcctttttc cagatcgtgt tggtaacaga caaacagtag
     8041 ccccatatcc agttgcccgg agttggtgac gcccagtgaa taactgtagc cacgacgcag
     8101 catcaggctg gactcactct ccgccgtgcg gggattcgcc agccggatat ggctgtccag
     8161 cgcaatcccc ttcccttccg ggtcgctggc gtaatcaggc acatcatgct catgctgcat
     8221 tcccagcggc gcaccggttt gcttatcacg gccaaaaatc gtctgctgtt ctttcagcgg
     8281 cgttctgtcc caaaattcca ctcgaaactg aatcaagcgt actgcctgat agctgccacc
     8341 gattgtccac gcaggctcct gctgatctgc cgttacccac accacttttt gcatcaactt
     8401 atcattctgg ctatcgggat tggcagtgcc gtctttgaaa cccagcaaat taatcggcgt
     8461 ctctttgcct ttactacgcg ccgcgtgatc ggaaataaac ccttcccgct tccagcgcac
     8521 gctgagcaaa tccggcgtat gtttgatgat atcgcgcagt gcatggatca ccgtgtcctg
     8581 ggtgttggcg caaatttgta gcaacacatc accatgacat aacgccgcat ccagcgagtc
     8641 gttgggaaaa cgcgtcatct tctgcaactt tttcggcatc tgtggcgcaa ggccaaagcg
     8701 ctcatcaaac aatgagtgac ccaccgataa cgtgatggtg agattatcgg gcgcaatgta
     8761 gccgccaaga atgccggaat cgagtggtgg caggcgcgga tttggcgttt ctggtgccgc
     8821 tccgccctga gtcagaaaag caaaacgctg agtcaacaag cgaaacaacc gttcaagatc
     8881 ggctttatcg ctggcaagca catcaaacgc caccagcatc attgcggcct gttgtggcgt
     8941 caggatccct gcctgatgct caccataaaa cggctgtttc tcattgcgtg caaccggtga
     9001 aagcgtaccc ggcgcacttt gcgttttttg tgcatgagcg accggacaac ttcccgccag
     9061 cgccagcgca ccaatgcctt tcagtaaacg tcggcgtgac ggttcattca cgccgttttt
     9121 atcttcatac tgcataatgc ttaatccaat cccagcacac cgcgaagttg cgccagatct
     9181 tccgccagcg cagtaatcgg tcctttcagt gcattccggt cggcatcggt caatttgtcg
     9241 taggtttcaa aaccgtcttt agtacggtat ttcgccagaa tggtatcgac ctttttaaag
     9301 ttggcatcga cttttgccaa cagttccggg ttagcttttt gcagttgtgg acgcagcaga
     9361 tcgacaattt tctgcgagcc ttcaacgtta gcctggaaat cccacagatc ggtgtggctg
     9421 tagcgatctt cttcaccgct gattttgctg gctgccactt cctcaatcag tccggctgca
     9481 ccgccgacca cttttgaagg tgggaaagcc agttcactga tgcgtttttg caaatcgacc
     9541 acatcggtat aaagctggtc agcgtactga tccatgcctt tggtggtgtt gtcgccaaac
     9601 aatgcttttt ccagacggtg gaaaccggtg aattttggat cagcggcttt ttgctcgtaa
     9661 tcatcttcac gggcgtcaat gctgccatcc agatcggaga acagttcagc aatcggttca
     9721 atgcgctcat agtgctggcg cgtcggcgca tacagtgctt tcgctttttc gatatcgcct
     9781 gctttaatcg cgtcggtaaa ggctttggtg tcggtcacca gctgtgtggt ttccgccatg
     9841 acatacgctt tatatgcagt aattgcacca ccaagactta acagcgcatc actttgcgcc
     9901 gcatccgccg ttgcctcacc tttgacgatc aacttccctt tcgggttagt cagcagaccg
     9961 caggtcatat cgtattcgcc aggctgtaaa ttcgccgtca ttttctggct aaagccaggg
    10021 gcgatatttt cccgctcttc caccaccatc acgcctttga ggatctccca ttccagcgcc
    10081 ttctggctgt ggttctgaat aatgaactgt gttttcccgg cgttaaccgt aatggtcatc
    10141 ggttcgcact gcttatccgt cacggtcact ttgacctgcg gcacatcagc ggcgttagcc
    10201 ataaaagcag aagaaaacag cgcagccacg ctcaactgca atgcgttacg gcggaagtta
    10261 atggtcatga cgagactatc cctttaaagt atgttgtaac taagcaagag tttgcgtcgt
    10321 atttgttacg cggagcgaga cgctgtcgcc cctgcgcgtg gtggcagagc aaatgccacc
    10381 agcgccggga tgagataaat aaaccagacg gcgacttcgc tgacgctcgg cgcttcctga
    10441 tagccaaaaa tcccttccat cagcgtgcca aacagcgagt gagttgagag caccgcactc
    10501 atatcgaagg cgatttcctg aaagtggttc cacaatccgg cttcatgaaa tgcgcgaatg
    10561 gcaccagctg ccagccctgc ggcgacgaag agaataaaca ggctggtcca tttaaaaaat
    10621 gcaccaagat tgaggcgaat accgccccag tagagcagga agcctagcac cacggcagta
    10681 gcaagaccga gcattgcacc cagcggcggc cagatcccga catcttgttg aaatgccgcc
    10741 agcaggaaaa agaccgactc cagcccttcc cttgcaacgg caaaaaagac catcatcacc
    10801 agcgcccagc catgatgatt tccacgctgc aatgcgctat cgacagcctg ctccagttgt
    10861 actttgacgt tgcgcgacac tttgcgcatc cagaaaacca tccaggtaag gatcaccacg
    10921 gcgatcaccg ccacgatacc ttcaaacagt tcctgctctt tttgcggaaa ttcgccggtg
    10981 gtttcgttaa tgaagatccc caagcccagg cacaacgcag cggcaagcaa cacgccaatc
    11041 cacatcacac caatccatcg gcctcgctgg gtacgcttaa gatagctggc aatcaaactg
    11101 acaatcagcg cggcttcaag tccttcgcgc aacataatga gaaacggaac aaacatgcca
    11161 cccaccctta aacgtttttc tgagtcaatc tatgcaaaga aaagtaaatc gcagtgatag
    11221 cgattatcat tacgctaaag aaaaatacaa gcggaatatt tataaaatga tgttggaatg
    11281 taaaaatgag gagatcaaat ccggagaaaa aagcgattat tttaaggtat ttagctgacg
    11341 tttcgcccgg cagatgaagc ttgtacgaat gttggtggcg acaaactcgc tgcagcgtgc
    11401 ccgaagggtt gaattttcag ttagtcgttt cacaaaagaa cgttgttttt tgcttattaa
    11461 tagatagtga taaggttgtg aatgaaatta tacctcatct cctcatcgaa ttttttgccc
    11521 acaacctaaa caggagaaaa cgtgtattta agtctggaat gagcgatttt tgatagtgcc
    11581 taat
//
LOCUS       DAZFDC010000134        11509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFDC010000134 DAZFDC010000000
VERSION     DAZFDC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.134"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            138..4040
                     /gene="espP"
                     /locus_tag="ACHJ48_003924"
     CDS             138..4040
                     /gene="espP"
                     /locus_tag="ACHJ48_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO4709382.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4296..4777)
                     /locus_tag="ACHJ48_003925"
                     /pseudo
     CDS             complement(4296..4777)
                     /locus_tag="ACHJ48_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            5483..5617
                     /locus_tag="ACHJ48_003926"
     CDS             5483..5617
                     /locus_tag="ACHJ48_003926"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709383.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            6219..7040
                     /locus_tag="ACHJ48_003927"
     CDS             6219..7040
                     /locus_tag="ACHJ48_003927"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO4709384.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            7040..8146
                     /locus_tag="ACHJ48_003928"
     CDS             7040..8146
                     /locus_tag="ACHJ48_003928"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO4709385.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            8236..9957
                     /gene="cptA"
                     /locus_tag="ACHJ48_003929"
     CDS             8236..9957
                     /gene="cptA"
                     /locus_tag="ACHJ48_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO4709386.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            10031..11029
                     /gene="lpxM"
                     /locus_tag="ACHJ48_003930"
                     /gene_synonym="msbB"
     CDS             10031..11029
                     /gene="lpxM"
                     /locus_tag="ACHJ48_003930"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO4709387.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            11082..11270
                     /locus_tag="ACHJ48_003931"
                     /pseudo
     CDS             11082..11270
                     /locus_tag="ACHJ48_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11374..11460)
                     /locus_tag="ACHJ48_003932"
                     /pseudo
     CDS             complement(11374..11460)
                     /locus_tag="ACHJ48_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3197 a         2496 c         2739 g         3077 t
ORIGIN      
        1 tgtttgttat ccgtatgata acaaacacat aaaggagtaa acatttatct ggacgatgat
       61 tacaatgttc acgactgtca gagacacacc tctgatacat tagaaaaacc aatgtttccc
      121 ttaaaaatgg agcttatatg aataaaatat actctcttaa atacagccat attacaggag
      181 ggttaatcgc tgtttctgaa ttatccggca gagtatcatc aagagcaact ggtaagaaaa
      241 aacacaaacg catacttgca ttatgttttt taggcttatt acaatcctca tattcttttg
      301 cgtcacagat ggatatttca aatttctaca tccgtgacta tatggatttt gcacagaaca
      361 agggcatatt tcaggctggc gcaacaaata ttgaaatagt gaagaaagat ggctccaccc
      421 tgaaactacc ggaagtacca tttcctgact tctcaccggt tgcaaacaaa gggtcaacca
      481 catctattgg tggtgcatac agtatcacag ccacacacaa tacgaaaaac caccactcag
      541 ttgcgacgca aaactggggg aacagcacgt acaaacaaac tgactggaat acttcacatc
      601 ctgattttgc agtatcccga cttgacaagt ttgttgttga gacccgaggt gcgactgaag
      661 gcgcagatat ttcgttatca aaacagcagg cacttgaacg ttacggggtt aattataaag
      721 gagaaaagaa acttatcgca ttcagagccg gctctggtgt ggtatccgtt aaaaaaaatg
      781 gacgcataac tccatttaat gaggtttctt ataagccaga aatgttaaat ggctctttcg
      841 ttcacattga tgactggagt ggatggctga tattaaccaa caaccagttt gatgagttta
      901 ataacattgc ctctcagggt gacagcggtt cagcactgtt cgtctatgat aaccaaaaga
      961 aaaagtgggt tgtcgctgga actgtctggg ggatttataa ttacgccaat ggcaaaaacc
     1021 acgcagcata cagtaaatgg aaccagacaa ccattgacaa cctgaagaac aagtattctt
     1081 acaacgtgga tatgtcaggg gctcaggttg caaccattga aaatggaaaa ctgacaggca
     1141 ctggctcaga caccaccgat ataaaaaata aggacttaat atttactggc ggtggagata
     1201 tcctcctgaa atcctctttt gataatggtg ctggcggtct tgtctttaat gataaaaaga
     1261 cctatcgagt aaacggggat gatttcacct ttaaaggtgc cggtgttgat acaagaaacg
     1321 gcagcaccgt tgagtggaat atccggtatg ataataaaga caaccttcac aaaattggtg
     1381 atggcacatt agatgtccga aaaacccaga acaccaacct gaaaacaggt gagggtcttg
     1441 tcattcttgg agctgaaaaa acattcaata atatctacat aaccagtggt gatggaactg
     1501 tccgactgaa tgcagaaaat gcactgtctg gcggtgaata caacggtatt ttctttgcga
     1561 aaaatggcgg aactcttgac ctgaacggat ataatcagtc tttcaataaa attgctgcaa
     1621 ctgattcagg tgctgtaata accaatacgt caaccaaaaa atccatttta tccctgaata
     1681 atactgctga ctatatctat cacggtaaca taaacgggaa tctggacgta cttcagcatc
     1741 atgagacgaa aaaagagaac cgtcgtctta ttcttgatgg gggcgtggac acaacaaatg
     1801 atataagcct gcgtaataca caactgtcca tgcagggaca tgccactgaa catgccattt
     1861 atcgggatgg agctttctct tgttcactac cagctcctat gcgctttttg tgtggcagtg
     1921 attatgttgc aggaatgcaa aatacagaag ctgatgctgt aaaacaaaac ggaaatgcct
     1981 ataaaaccaa caatgctgtc tctgatttat cgcagccaga ctgggaaacc ggaacattca
     2041 gatttggaac gctacatctt gaaaattccg atttttctgt tggtcgtaat gcaaatgtaa
     2101 tcggggacat tcaggccagt aaatcaaaca ttactattgg tgacactaca gcatatattg
     2161 atttgcatgc tggtaaaaat attaccggtg atggttttgg cttccgtcag aatattgtgc
     2221 gtggaaactc acaaggagaa acgctgttta caggagggat cacagcagaa gacagcacta
     2281 tcgttattaa agataaagca aaagcattat tttcaaatta tgtatacctg ctgaacacaa
     2341 aagcaaccat agagaacggt gctgatgtga caactcaaag tggtatgttc tccacgagcg
     2401 atatcagcat ctctggtaat ctgtccatga caggcaatcc cgacaaagac aataaattcg
     2461 agccctcaat atatctgaat gatgcttctt atctactgac tgacgactcc gccagactcg
     2521 ttgccaaaaa taaagcatct gtggtgggag atatacactc cactaaaagt gcatccatca
     2581 tgtttggtca tgatgaaagc gacctctcgc agttgtctga cagaacctca aaagggcttg
     2641 cacttggtct tttaggtggc tttgatgtct catatcgcgg ttcagtcaat gccccgtcag
     2701 catctgccac tatgaacaac acctggtggc aactaaccgg agattctgcg ctgaaaacac
     2761 tgaaaagtac aaacagcatg gtctatttca ctgacagcgc aaacaataag aaattccata
     2821 cgctgacggt cgatgagctg gcaaccagca acagcgccta tgcgatgcgt acaaaccttt
     2881 ctgaatcaga caaactggag gtcaaaaaac acttgtctgg tgagaacaat attttactcg
     2941 ttgatttcct tcagaaacca acgcctgaaa aacaactgaa tattgaactg gtaagcgcgc
     3001 caaaagacac caatgaaaat gtctttaaag ccagtaaaca aaccattggt ttcagtgatg
     3061 taacgccggt cattacaacc agggaaaccg atgacaaaat aacatggtca ctgacaggct
     3121 ataacacggt agcaaacaag gaagcaaccc ggaatgccgc cgccctgttc tctgttgact
     3181 ataaagcgtt tctgaacgag gtcaacaacc tgaacaaacg tatgggtgac ctgcgtgata
     3241 tcaacggcga agccggtgca tgggcacgca tcatgagcgg taccggctct gccagtggtg
     3301 gtttcagtga caactacacg cacgttcagg tcggggtcga caaaaaacac gagctggacg
     3361 gactggattt gtttaccggt ttcactgtca cacacactga cagcagtgcc tccgccgatg
     3421 ttttcagtgg taaaacgaag tctgtggggg ctggcctgta tgcttccgcc atgtttgatt
     3481 ccggtgccta tatcgacctg attggcaagt atgttcacca tgataatgag tacactgcaa
     3541 cctttgccgg actcggaacc cgtgattaca gcacgcattc atggtatgcc ggtgcagaag
     3601 cgggctaccg ctatcatgtc actgaggatg cctggattga gccacaggct gagctggttt
     3661 acggttctgt atccggtaaa cagtttgcat ggaaggacca gggaatgcat ctgtccatga
     3721 aggacaagga ctacaatccg ctgattggcc gaacgggtgt ggatgtgggt aaatccttct
     3781 ctggtaagga ctggaaagtg acagcccgtg ccggtctggg ctaccagttc gacctgctgg
     3841 ctaacggcga aaccgtattg cgggatgcat ctggtgaaaa acgcatcaaa ggtgaaaagg
     3901 acagccgtat gctgatgtcc gttggcctga atgcagaaat cagggataac gtccgctttg
     3961 gactggagtt tgagaaatcc gcctttggta agtacaacgt tgataatgct gtcaacgcta
     4021 atttccgtta ctcgttctga tactccgtat tcaaaagggc ctgcgggccc tttacgctca
     4081 gtgcctgccg ccctgatatg tctgagagac atttccggga tgtcagtcga ctggtttact
     4141 taactgcgat gactaaagct ggcagtaatg ccggcttttt tacggccaca tacgtcttcc
     4201 tctcatccgg aactgtgata caaatcctgc atacaggatt gaccgccggt tcctgcaaat
     4261 ggcacatttt cagtaagata gcgaaggtta catttgtcac tggttaacat accgatggta
     4321 aaaggggcaa gcagcgccag caattcatca gcccgggggc caaaagtgta ggccagaacg
     4381 ccaccagttt tcgtgttgta cgcgtaccag agcctgtgct gtcacgcttt actgccgata
     4441 aaactccatt gctcatcaag ttcgcagatg agtgccatat cagcatgagc gaccggggat
     4501 gacgttattc ttcgtggcga gcgtttttta aagtccgaat gacagtatta atgccgattt
     4561 tcagcgtacg ggcagtatcg cgaactccgg caccgttgaa aggcatttca gtgatttggt
     4621 cttgaactcc cggtttacgg gcctcgtaac ggtaagtgag ctgaaacact cgatgacagt
     4681 cacggcagcg aaatcgatca tgtccttttg ggttcttccc gtgacggtaa acctgaacgg
     4741 actggcaacg ggggcaatga aggttaactg tgaccattaa agaaccttaa aagtgactat
     4801 tataccccta attcaactaa ttggaggcat taccatgcat tagtgcaagc gccttgtgga
     4861 ttcttttgct aatggcggaa acatattgta caatattgca tttatccatc aataaataaa
     4921 tgagttatca gattgcaaaa atctgttatc aaactaattc aaataaaaag agattgatac
     4981 gaccaacgta cacaaaaatt ttcgatattc gaactaaata acagctccta ataccgctac
     5041 actttagcta tccagtctgc gcctcagaaa tggacgatgc cgttgaagga ctggtgtatg
     5101 gcaatgagcc gctttattat cgaattcggt gaccgcttgg acggtcactt cggagaaaag
     5161 gcatttacac agcatcctaa acaggttcag cataggtgat ataccgcctt ttcattttct
     5221 tctccattat ttataaggga ggtgtattat tatgcaatat tattggtttt atccattgtt
     5281 tttagttcat gaatgtttgc aaaaaaaata aatgaacaat aattttatgt atttaaaata
     5341 aattgatttt acttctgtaa tggtgtcatg attagcagct ttttaaacta atatcgactt
     5401 tcaataaata aggacaggta atggacaaca aaaatatctg aacccgggtg agattcataa
     5461 gttgcacagt gctgtcttca aagtgccaca cccggaacgt aatcactgcc tgctccttat
     5521 ggggtatctt catggtgtcc aagcatcaga gttattaggt ataaaactgt ctgatattga
     5581 tttgcaggcc ggtaacctga atatccggcg gctctaaacg gtttgtcaac tcagcccctc
     5641 ggctgaaaca ggaggtacag tccattcgtc gctggctgaa acttcgggat gaatgggtag
     5701 aagagggata tgaatggtta tttaacctct atggcaggtc tgctacaggt attactgttg
     5761 ttctgggggg cactggtgtg gttaatatct acgatgcaag agattctcac gatggcccgg
     5821 tccatcagtg catgcggcgc gatattgtgc gagtattttt cagggggagt ggtatccgga
     5881 tgcagaaaat tccgctactc gactggtttt atgcaataaa tcaggatgat aatcttctga
     5941 ttttttactg aggcggatat ctctcgctgt gtttactgaa attgcggtat tctgtttttt
     6001 ctgaaaaaca gaaaatctga ttaaaaagag catatgcgtt aagggaaatt taagttctgg
     6061 ttgattaact gataaaaata ttaaaaaatc cctgttgtcg gtaatctccg gaccattccg
     6121 ggattacaca ataatatcac catgtgtgat atctatggat cctcactgtt gtttacgcga
     6181 ggatattttc agaatgctta acagacgatt atttttttat gttgaatgca aggcatcttc
     6241 ccgtactgat gtatcatcat gtcagtcgtt gtccggggct tgtgacactc tcacctgaaa
     6301 cattttgtga acagatgaag tggctcgcag ataataactg gcggacggta acatctgctg
     6361 agcttgaata tttttatcag gggggaacgc ttccacgaaa gagtgtcatg ctcacattcg
     6421 atgatggtta tcttgataac tggctcagag catggccggt cctgaaggaa tataaccttc
     6481 atgcacatat ttttctcatt acggggctga ttggtaaggg aaacgtacgt agcaggcagg
     6541 agcaggaata ttctcaccgc gactgtgaac ggttgattgc cgataaccgt tcagatgagg
     6601 tcatgcttcg ctggtctgaa gtccgggaaa tgcgtgattc cggactggtt gaatttcatg
     6661 tgcacaccca ttcacataaa cgctgggaca ggttgtctgt atcacgtgct gaacagtgcc
     6721 ggctcatgaa agaagatatt ctggtgggaa aacaatgtct gacggaaaag ctggggttct
     6781 gtagttccca tctgtgctgg cctgaaggat attataatcg ggattatatt aatctcgccg
     6841 gtaagcttgg tttctcttat ttatatacaa ccgaaagaag aatgaactgt cctgaaaatg
     6901 gttcattacg catagggcgt atcagcacca aagagcggga acattccggc tggctgaaac
     6961 gacgcctttt ttattataca acgccacttt tttcttcagt actggctctt cataaggggc
     7021 caagattacc agataattaa tgatgaaaat tttattcact gaatcttcgt cagatattgg
     7081 gggccaggag ctgcaggcac tggcccagat gactgctctt caaaagcagg gacattccgt
     7141 tttacttgcc tgcagagaaa aaagtaaaat tgctccggaa gccagaaaaa gagggcatga
     7201 tgtcactttt attcctttca ggaacagcct tcaccttcct tccatcctca gacttcgacg
     7261 gattatcggg gaatttaaac ctgatctggt catctgccat agtggccatg acagtaatat
     7321 agcaggactg agccgtctga tatgctgtca ccgcttcagt attgtcagac aaaaaaccta
     7381 catcacccgt aagaccagaa cgttttccct gaattatctg tgtgatttca ttgttgtgcc
     7441 tagctcagcc atgatggcgc atctgatggc ggaaggtgtc aggacaccgg taacggttat
     7501 tccaccgggt ttcgactggc ccgcattgca taatgaagcc atgcgaccgt tacccctgca
     7561 tatccacgcc tgggctgcat cagcagacaa tgttccgctt atcgttcagg tggggatgct
     7621 acgtcccgaa aaaggacatg aatttatgct gcgtgtattg tatcagctga agatggaagg
     7681 gaaatcattt cgctggctcg tggtgggcgc cgggcgtgaa gaatacgaag cacgtctgcg
     7741 tcagcagaca gaacacctgg gaatgagcgg agatgtgtta atggcaggtg cccttttccc
     7801 agcgttaccg gtctaccgga ttgccagtgt ggtggtcatg ccttcggaaa atgaagcgtt
     7861 tggtatggta ctggctgaag cgtcagtctc cggcgtgccc gttatcgcca gtgagacggg
     7921 cgggatccct gatgtcattc agaagaatgt gacagggacg ctgttacccg tgggggatgt
     7981 gtctgcatgg accggcgcac tgcgcgattt tttatcccga ccggaacgct tccggatgat
     8041 ggcagccagc gccagagaag atattgagta ccggtttgat attaacagaa cggcacagat
     8101 cattgtgtct ctggctagtc aggctaaggg gaaatgcaac agataacggc ggatcctctt
     8161 ttcagcccga attttgttca gcggttttcc ggtaacggag taatttatca cattgtttaa
     8221 atacggggac ggtcaatgca tttaaatacc ggacagaaca ggcctacttt cagctggagc
     8281 gcgctgggat gggcaatttt ttattttggc tttttttcca ctctcctgca ggtcatcatc
     8341 ttcagcagtg ggtacagcgg aacgaatgga atacgggact cactgttatt cagttgtctg
     8401 tggttgatcc cggtgtttct ctatcctgat cggataaaaa taattgcagc tgttgtcggt
     8461 ttcattctct ggggcacgtc gctggcagca ctttgttatt attttctcta tggtcatgaa
     8521 ttctctcaaa gtgttctttt cgttatgttt gaaacgaatg ccagagaggc tggtgaatat
     8581 ttcagccagt atttcagcct taaactgttg cttatctcgc tggtatatac tgcggtgtcc
     8641 gtttttctgt ggacacgtct gcgtcctgta tatattcctt tgccatggcg gagaattgtc
     8701 tctttcctgc tgctttatgc tctgcttctg catccggttg ttctgaaatc gttaatcaga
     8761 caggagccgc tgaatgatac tctaggcaaa ctggcatccc gaatggagcc tgctgccccc
     8821 tggcagtttg tatccagtta ttatcagtac catcagcaac tgaatgcact gacaaccttc
     8881 ctgaatgaaa atagcgcact gccaccactg ggtaatctca gggatgaatc aggggagaga
     8941 ccacgcacac tggtcctggt gattggtgag tcgacacagc gcgaacgcat gagcttatac
     9001 gggtatctac gtgaaacgac gccggagctg gatgcactgc gtaaaaccga tccgggtctt
     9061 actgtgttta ataatgtggt ggcatcgcgt ccgtacacca ttgaagcatt gcaacaggcc
     9121 cttactttcg ccaacgaaaa gaatcctgat ctgtatctga cgcagccgtc gctgatgaac
     9181 atgatgaagc aggcaggcta taaaacattc tggattacca accagcagac aatcacagcc
     9241 cgtaacacca tgctcactgt attttcgcgc cagacggaca ggcagtacta catgaatcag
     9301 cagcgaacac aaagtgcgcg tgaatatgac actaacgtgc tgaagccgtt ccgggaagtg
     9361 ctgaatgacc ctgcaccaaa gaaactgatc atcgtgcatc tgctgggtac gcacattaag
     9421 tataaatacc gttatccgga aggtcagggg cggtttgatg gcattacagg gcatattccc
     9481 actggattaa atgcgaaaga gctggaagtg tataacgatt acgataatgc caatctgttt
     9541 aacgatcatg tggtggccag tctgataaag gacttcagag caactgcgcc ggacggcttt
     9601 ctgctttatt tttcagacca tggtgaagaa gtatatgata ctccgccata taaaacgcag
     9661 ggacggaatg aagataaccc cacacgtccc atgtacactg ttccgttcct gctgtggacc
     9721 tcggaaaagt ggcatgctgc acatccgcga gatttttcgc agtatgttga ccgcaaatac
     9781 agtctggctg aactgatcca cacctggtca gatttggcgg gactgacata cgatggttac
     9841 gatccgaccc gttctctggt gaatccgcaa ttcagggaaa ccacccgctg gattggaaat
     9901 ccgtataaaa agaatgggct cactgatttc gacactcttc cgtatggtga gccatagtat
     9961 tttcttatct tcggttccgt tctgaaggca ggaggatgtg ttttcctctg ctgtccggtg
    10021 gcctccaggg atggtaccac cttctgctgt actctgttat cataatgaga taagcaggca
    10081 gataccggta aatatgaaaa atatcagaac tgagtttatt ccccgcttta accttacatt
    10141 atgcttcccc cgttactgga tgacgtggac aggtatcggt attatctgtg tgtttgcaat
    10201 ggtccccccc gcactgagag atccattgtt gggaaaattg ggcatgctgg ttgggcgtct
    10261 ggggaaaagc gcacgtcaac gggccttgat taatttatcg ctttgttttc cggagtacag
    10321 tgataaagaa aaggagaata tcgttgatgc catgtttgca acagcctcca tggccgttgt
    10381 gcttatggct gaattagcac tgtccgggcc tgataaaatt tcgcatcgta tacgctggaa
    10441 tggtctggag attgtggaga agatggcaca gaacaatgaa aaggtcattt ttcttgtgcc
    10501 acatgcctgg ggagtggata ttcccgcaat gctgatggct gcgtcgggca gaaaaatggc
    10561 agcgatgttt cataaccaga gaaatccggt ggtggattat gtctggaaca gtgttcgtcg
    10621 tcgtttcgga ggaaaactgc acgcacgtaa tgacggtatt gcctcctttg tccgctcagt
    10681 ccggcaggga tactggggat attatcttcc tgatcaggat catggcccgg aattcagtga
    10741 gtttgcagat ttttttgcga cgtataaagc aactttaccg gttatcggac ggctcagcag
    10801 gatcagtggt gcacggataa taccgctttt ccctgtttat gacggaaaga cccatcatct
    10861 gactattcat gtcagcccgc cacttgctat acgacagaag agtgatgctc atattgcgcg
    10921 acagataaac gaagtggttg aaaatttcgt tcgtccacat cctgagcagt atacgtggat
    10981 cctcaagttg ctgaaaacgc gtaaagaagg ggaagaggat ccttactgag gcagcttttt
    11041 caggataatg agcttaccca gcaatagtgg acacacgcta agtgagtaaa ctctcagtca
    11101 gaggtggctc atatgacaaa aaaacagtac caaccagtaa aaaaccccgt aaatagcatt
    11161 cgcctgaatt tcgcagtgaa gccctgaagc ttgctgaacg catcggtgtt gctgtcgcag
    11221 ctcgtgtgct cagtctgtat gaatcacagc ttgtaagcgg ctcgtcagaa ctgtattgat
    11281 atttactgag cggtcagttc aacgtaagcg agtgttatgc gccatggaag cttacccagg
    11341 aatatgttat caggcctcag catggcgaag tgttgcaacg ctggaagcag aatgaccgcg
    11401 gtcaacaact tgttttaccg cttcaatttt aaaatcttcg ggataacgct taccgctcat
    11461 gggcacctct ctttaagcca tttaaaatga ctctgaggtg tctgttaaa
//
LOCUS       DAZFDC010000135        11508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFDC010000135 DAZFDC010000000
VERSION     DAZFDC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.135"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            8..2434
                     /gene="ynfE"
                     /locus_tag="ACHJ48_003933"
     CDS             8..2434
                     /gene="ynfE"
                     /locus_tag="ACHJ48_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO4709388.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            2495..4918
                     /gene="ynfF"
                     /locus_tag="ACHJ48_003934"
     CDS             2495..4918
                     /gene="ynfF"
                     /locus_tag="ACHJ48_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO4709389.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4929..5546
                     /gene="ynfG"
                     /locus_tag="ACHJ48_003935"
     CDS             4929..5546
                     /gene="ynfG"
                     /locus_tag="ACHJ48_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO4709390.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5548..6402
                     /gene="ynfH"
                     /locus_tag="ACHJ48_003936"
     CDS             5548..6402
                     /gene="ynfH"
                     /locus_tag="ACHJ48_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO4709391.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFVTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6445..7059
                     /gene="dmsD"
                     /locus_tag="ACHJ48_003937"
     CDS             6445..7059
                     /gene="dmsD"
                     /locus_tag="ACHJ48_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO4709392.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7217..8509
                     /gene="clcB"
                     /locus_tag="ACHJ48_003938"
     CDS             7217..8509
                     /gene="clcB"
                     /locus_tag="ACHJ48_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO4709393.1"
                     /translation="MHHLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(8462..9157)
                     /gene="bioD"
                     /locus_tag="ACHJ48_003939"
     CDS             complement(8462..9157)
                     /gene="bioD"
                     /locus_tag="ACHJ48_003939"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO4709394.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9282..10502)
                     /gene="mlc"
                     /locus_tag="ACHJ48_003940"
                     /gene_synonym="dgsA"
     CDS             complement(9282..10502)
                     /gene="mlc"
                     /locus_tag="ACHJ48_003940"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO4709395.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10637..>11508)
                     /gene="ynfL"
                     /locus_tag="ACHJ48_003941"
     CDS             complement(10637..>11508)
                     /gene="ynfL"
                     /locus_tag="ACHJ48_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4709396.1"
                     /translation="RYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLAR
                     TNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVS
                     DTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPL
                     MAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEV
                     GEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPA
                     ARNFRIHLLNALR"
BASE COUNT         2783 a         2987 c         2956 g         2782 t
ORIGIN      
        1 gtgagttatg tccaaaaatg aacgaatggt gggcatcagc cgcagaacac tcgttaaatc
       61 taccgcgata ggttctctgg cgctggctgc aggcggtttt tctttgccgt tcaccctgcg
      121 cagtgcagcg gcagcagtac aacaggccag cgaacaagtg gtctggggtg cctgttccgt
      181 caactgtggt agccgctgtg cacttcgtct acatgttaaa gataatgaag tggcctgggt
      241 ggaaactgac aacaccggca gcgatgagta tggcaaccat caggtacgcg cctgtttgcg
      301 cggtcgctcc atccgccggc gtattaatca tcccgatcgc ttgaattacc caatgaaacg
      361 cgtgggtaaa cgcggtgaag gcaaattcga acggattagc tgggatgaag ccctggatac
      421 tatcgccagt agcctgaaga aaaccgtcga acaatatggc aatgaggctg tatatattca
      481 gtactcttcg gggatcgttg gcggcaatat aacccgctct tcgccatcag cctcggcggt
      541 caaacgcctg atgaactgct acggcggttc actcaaccag tatggctcct acagcactgc
      601 gcaaatttcc tgtgccatgc cctacaccta cggcagtaat gatggcaaca gcaccacgga
      661 tattgaaaac agcaagctgg tcgtgatgtt tggtaacaac ccggcagaaa cccgcatgag
      721 cggcggtggc atcacttatc ttcttgaaaa atcccgcgaa aaatcgaacg caaaaatgat
      781 tgttatcgat ccgcgatata ccgatacggc tgctggtcgt gaagatgaat ggcttcctat
      841 tcgcccgggc accgatgccg cgctggttgc gggtattgcc tgggtattga ttaacgaaaa
      901 tctcgttgat caaccttttc tcgataaata ctgcgtcggc tatgacgaaa aaaccttacc
      961 cgcagatgca cccaaaaatg gtcactataa agcctatatt cttggtgaag gtgacgataa
     1021 aacagcgaaa acgccgcagt gggcttcgca aattaccggt atcccggtgg accgtatcat
     1081 caaactggcg cgtgaaattg gcacagcaaa acccgcttat atctgccagg gctgggggcc
     1141 gcaacgccag gcaaacggcg agctgaccgc acgcgctatt gctatgttgc ctattctgac
     1201 gggcaatgtc ggcatcagcg gcggaaatag tggcgcgcgt gaatcgacct ataccattac
     1261 catagagcgc ctgccggtgc tggataatcc ggtcaaaacg tcaatttcct gcttcagctg
     1321 gacagatgct atcgatcatg gcccacaaat gacggcaatc cgcgacggtg tccggggcaa
     1381 agataaactg gatgtgccca ttaagttcat ctggaactac gcgggaaata ccctggttaa
     1441 tcagcattct gacatcaaca aaacgcatga aattctgcag gacgaatcga aatgcgaaat
     1501 gattgtggtc atcgaaaact ttatgacctc atcggcaaaa tatgccgaca ttctgctgcc
     1561 agacctgatg accgttgagc aggaagatat tattcctaac gactacgccg gtaacatggg
     1621 atatctcatt ttcctccagc ctgtcaccag cgaaaaattc gaacgcaaac cgatttactg
     1681 gatcctgagt gaagtcgcga aacgtcttgg acctgacgtc tatcaaaagt tcactgaagg
     1741 tcgcacgcag gaacaatggt tacaacatct gtacgccaaa atgcttgcca aagatccggc
     1801 gttaccgtct tacgacgaac tgaaaaaaat gggtatctat aagcgtaaag atcccaatgg
     1861 tcattttgtc gcctacaaag catttcgtga cgaccccgag gcaaatccac ttaaaacacc
     1921 ttccggtaag attgaaattt attccagcaa gctggcggaa attgcccgta cctgggaact
     1981 ggaaaaagat gaagtgataa gcccactgcc agtttatgcc tcaacctttg aaggctggga
     2041 ctcccctgaa cgtagtacct ttccactgca actgtttggt ttccattaca aatcccgtac
     2101 tcactcgacc tacggcaaca ttgatgtcct gaaggctgcc tgccgtcagg aggtgtggat
     2161 caaccctata gacgcacaga aacgtgggat tgccaacggc gatatggtgc gggtgtataa
     2221 ccatcgtggc gaagttcggc taccagccaa agtgacacca cgcattctcc ctggggttag
     2281 cgctatgggc cagggagcct ggcacgaggc caatatgtct ggcgataaaa tcgaccatgg
     2341 cggatgtgtg aatacgctaa ccactctgcg cccttcacca ctggcgaagg gaaacccgca
     2401 gcacacgaat ctggtcgaga tcgaaaaaat ataacccact acaaccataa attctggcat
     2461 gacattttgt ttgaaaagca ataagtgagt aatgatgaaa atccatacca cagaggcgct
     2521 catgaaggct gaaatcagcc gtagaagtct gatgaaaacc tccgcacttg gcagtcttgc
     2581 gctggcaagc agtgctttca ctctgccatt ttcccaaatg gtccgagcgg cagaggcacc
     2641 ggtagaagag aaagcggtct ggagttcctg caccgttaac tgcgggagcc gctgtctgtt
     2701 acgtttgcat gtgaaagatg acaccgtgta ctgggtggag tctgatacga caggtgacga
     2761 cgtctacggt aatcatcagg ttcgtgcgtg tttacgcggg cgctctatcc gccgacggat
     2821 gaatcatcct gataggttga aatatcccat gaagcgcgtc ggcaagcgcg gtgaaggtaa
     2881 atttgaacgg ataagttggg acgaagccct ggataccatc agtgataatc ttcggcggat
     2941 cctgaaagat tacggcaatg aggctgtaca tgtcctgtac ggaacaggcg tagatggcgg
     3001 aaacatcacc aactcaaacg tcccgtaccg tctgatgaac tcttgcggtg gttttctcag
     3061 tcgctatggc agctacagta ccgcacagat cagtgccgca atgagttata tgttcggtgc
     3121 caatgatggc aacagcccgg atgatatcgc caatacgaaa ctggtcgtta tgttcggaaa
     3181 taacccggca gaaacgcgga tgagcggtgg tggtgtcact tactacgtcg agcaagcccg
     3241 cgaacgttca aacgcacgca tgatcgtcat cgatccacgt tataacgaca cagctgccgg
     3301 gcgtgaagat gaatggctgc ccattcgccc tggcaccgat ggcgcactgg cctgtgcgat
     3361 tgcctgggta ctgattagtg aaaacatggt cgatcagcca tttctcgaca aatattgtgt
     3421 tggttacgat gaaaaaacgc tgcccgccaa cgcaccacgt aacgcgcatt ataaagccta
     3481 tattctgggc gaagggcctg acggcatagc taaaacgccg gaatgggcag caaaaatcac
     3541 cagcatcccg gcagaaaaaa ttatccagtt ggcacgagag atcggttcag caaaacctgc
     3601 ttatatttgt cagggttggg ggccacaacg acattccaac ggcgagcaaa catcccgcgc
     3661 tattgccatg ctttccgttc tcaccggtaa cgtcggcata aacggcggca actcaggtgt
     3721 acgcgaaggt agctgggatc tgggggtaga atggttcccg atgctcgaga atcctgttaa
     3781 aacgcagatt tccgtcttta catggacaga tgccatcgac catggtacgg aaatgaccgc
     3841 gacccgtgat ggtgttcgtg gaaaagaaaa actggatgtc cccatcaagt ttttatggtg
     3901 ctacgccagt aacacattga tcaatcaaca tggcgacatc aatcacacca atgaggtgct
     3961 tcaggatgac agcaagtgcg agatgattgt tggcattgac cacttcatga cggcctcggc
     4021 taagtattgc gatatcctgt tgcccgacct gatgccaaca gagcaagaag accttatctc
     4081 tcatgaatct gcagggaata tgggctatgt gatcctcgcc caacccgcaa cctcagcaaa
     4141 atttgaacgc aaacccatct actggatgct gagtgaagtc gccaaacgct taggaccgga
     4201 cgtttatcaa acctttactg aaggtcgcag tcagcatgaa tggatcaaat atctccatgc
     4261 gaaaacgaag gaacgtaacc ctgagatgcc cgactacgag gagatgaaaa cgaccgggat
     4321 ctttaagaaa aaatgcccgg aagaacacta cgtcgctttc cgcgcattcc gtgaagatcc
     4381 acaggcaaac ccgttgaaaa caccttcggg gaaaatcgaa atttattctg aacgactggc
     4441 gaagattgca gatacctggg aattgaaaaa agatgaaatt attcatcccc ttcctgcgta
     4501 taccccaggt tttgatggct gggacgatcc cctgcggaaa acctatccac tgcagttaac
     4561 gggcttccac tacaaagcgc gtacccactc cagctacggc aatattgatg tgttacagca
     4621 ggcctgccca caagaggtgt ggatcaaccc cattgatgct caggctcgcg gtatccgtca
     4681 tggcgatacc gtgcgggtat ttaacaataa tggagaaatg ctgattgccg caaaagtgac
     4741 tccgcgtatt ctgcctggcg ttaccgccat cgggcaaggt gcgtggctta aagcggatat
     4801 gtttggtgac cgggtcgatc acggcggcag tatcaatatt ctgacctctc accgcccttc
     4861 accgctggca aagggaaacc cgtcgcacag caatcttgtc cagatcgaaa aggtttaagg
     4921 agtagcccat gaccacacaa tatggatttt ttatcgattc cagccgctgt actggctgta
     4981 aaacctgcga actggcgtgc aaagatttta aagatcttgg cccggaagtc agtttccgcc
     5041 gtatttatga atatgctggc ggcgactggc aggaagataa tggcgtctgg caccagaacg
     5101 tgttcgctta ttatctctcc atctcctgta accattgcga tgaccccgca tgtacaaaag
     5161 tctgccccag cggcgcaatg cataagcgtg aagatggctt tgtggtggtt gatgaagatg
     5221 tttgtatcgg ctgccgctac tgccacatgg cttgtccgta cggcgcgcca cagtacaatg
     5281 ctgaaaaagg gcacatgacg aagtgcgatg gttgttattc gcgcgtcgcc gaggggaaac
     5341 aacccatatg tgtcgaatcc tgcccgctgc gtgcacttga gttcggtcca attgaagagc
     5401 tgcgccagaa gcacggcact cttgctgccg tcgcgccact gccgcgcgcg catttcacaa
     5461 aacccaatat cgttatcaaa cctaacgcca acagccgccc gaccggcgat accactggtt
     5521 atctggctaa tccggaggaa gtgtaaaatg ggaaatggat ggcatgaatg gccactggtt
     5581 atctttaccg tattgggtca atgcgtagta ggcgcgctga ttgttagcgg aattggctgg
     5641 ttcgccgcca aaactgacgc agaccgtcag cacattgttc gtggcatgtt tttcctctgg
     5701 ctgttgatgg gcatcgggtt tattgcctct gtcatgcacc tgggatcgcc actgcgggcg
     5761 tttaactcgc tgaatcgaat cggagcttct ggtctgagta acgagattgc tgccggttcc
     5821 atattttttg ccgttggcgg actatggtgg ctggtggcgg tcatcggtaa aatgccgcaa
     5881 gcgttgggga aactctggtt acttgtcagc atggcgctcg gcgtcatttt cgtctggatg
     5941 atgacctgcg tgtatcaaat cgacactgtg ccaacctggc ataacggtta cactacgctg
     6001 gctttcttcg tgacggtatt gttgagtggt ccaatacttg ctgccgcaat tctgcgggca
     6061 gcccgcgtta catttaatac cacaccattt gccatcatta gcgttctggc gctgatcgct
     6121 tgcgccgggg tgattgtgct gcaaggtttg tctctgtcgt ctattcactc atccgtgcaa
     6181 caagccagcg cactggtacc agattacgcc tccttacagg tctggcgtgt ggtattgtta
     6241 tgcgcaggtc taggatgctg gctttgtccg ctcatacgtc gccgtgaacc tcacgttgcc
     6301 ggacttattc tggggttaat cctcattctt ggcggtgaga tgatcgggcg tgtgctcttt
     6361 tatggcctgc acatgacggt aggaatggct attgcaggtt aacaaattgg tgcggggcaa
     6421 cccgcacatt tcaggatgtt aggaatgacc catttttcac agcaagataa tttttctgtc
     6481 gcggcgcgcg tgttgggcgc gctgttttat tacgctccag agagtgctga agccgcacct
     6541 ctggttgcgg tactcaccag tgacggctgg gaaactcagt ggcctctacc agaagcgtca
     6601 ttagcgccgc tggtgacggc ttttcagaca cagtgtgaag agactcacgc ccaggcctgg
     6661 cagcgtttgt ttgtcggccc gtgggcactg ccgtctccgc cgtgggggtc ggtctggctg
     6721 gatcgcgaat ctgtgctgtt tggcgattca acattggcac ttcgtcagtg gatgcgcgag
     6781 aaaggcattc agtttgaaat gaagcaaaac gaaccggaag atcattttgg ttcactgctg
     6841 ttgatggctg cgtggttggc ggagaatggt cgccagacag aatgcgaaga attgctggca
     6901 tggcaccttt ttccctggtc aacacgtttt cttgacgttt ttatcgaaaa agcagaacac
     6961 cctttctacc gtgcactggg tgaactggct cgcttaacgc tggcgcaatg gcaatcacaa
     7021 ctgttaattc ctgtcgcggt taaaccactc tttcgatagg atcaccgtaa tattgccgga
     7081 tggtgacgat tacgcgcgat tcggcaacaa tacgttacag tgtcacgttt tttgtctctt
     7141 aaagcacgca ctgcttttgc agctggcctc ttttgccgca aaatagtcgc ccgtgtttca
     7201 ttgcccattt ctgctcatgc atcatctaca cacctatccg gatctgcgca ctatgttccg
     7261 ccgtctgctt atcgcaacag tcgtcggtat tctcgcggcc tttgccgttg ccgggtttcg
     7321 tcatgcgatg ctgctactgg agtggttgtt cctcaataat gactccggca gtctggtcaa
     7381 tgcagcgaca aacctttccc cctggcgacg gctgctaact ccggcgctcg gcggactggc
     7441 ggcgggtttg ttgttgatgg gctggcagaa atttacccag caacgccctc atgcgccgac
     7501 cgattacatg gaagcgttgc aaaccgatgg tcagtttgat tacgcagcaa gcctggttaa
     7561 atcgcttgcc tctctgctgg tagtaaccag cggcagtgca attggtcgcg aaggtgcgat
     7621 gattctttta gctgcccttg ccgcctcctg ttttgcccaa cgttttacgc cacgccagga
     7681 gtggaaatta tggatcgcct gtggggccgc ggcgggaatg gctgcggcct atcgtgcccc
     7741 gcttgctggc agtttattta tagccgaagt gctgtttggc actatgatgt tggcctctct
     7801 cggcccggtg attatttccg ccgtcgtggc attgctggtt agcaatctga ttaatcatag
     7861 cgacgcgtta ctctacagcg tacaactctc agtgacggtt caggctcgtg actatgcgct
     7921 gattatcagt acaggtgtgc tggcaggtct gtgcggacca ctgttgttaa cgttaatgaa
     7981 cgcctgtcat cgtggatttg tgagtctcaa acttgcgccg ccctggcaac tggcactagg
     8041 cgggttgatc gtgggtctgc tttccctgtt cacacctgca gtgtggggca acggctatag
     8101 caccgtacaa tcctttttaa ccgccccgcc actgttaatg atcattgccg ggatcttcct
     8161 ttgtaaactg tgtgccgtgc tggcgagtag cggttccggc gcacccggtg gtgtctttac
     8221 accgacgcta tttatcggtc ttgccattgg catgttgtat ggtcgtagtc tgggattatg
     8281 gttccctgat ggcgaagaaa ttacgctttt actcggattg accgggatgg cgacactgtt
     8341 ggcggcaacc acgcacgcgc cgattatgtc gacgttgatg atatgtgaaa tgaccgggga
     8401 gtatcagcta ctccccggtt tattgattgc ctgcgtaatt gcgtcggtaa tctcgcggac
     8461 gttacaccgt gactctatct accgccagca cactgcgcag catagctaag cggatgtatt
     8521 gccccagttc acgctgttcg gcgcgcggca gataaggcag ttcaccaatg agcggtgccg
     8581 gaagtttttt accgagcaca tcaatgattt ccgcataatg cgccagtcct gggttgattc
     8641 ggttagccac ccagccaatg agcggcagcc cgtcgttggc aatcgcctga gctgttagca
     8701 gtgcatggtt aatgcaacct tcctgaatac cgacaaccat caacaccggc agttgttcct
     8761 gcaccaccca ttcagagagt ggacgcaaat cattcatcag actacgccag ccgccagtcc
     8821 cttctaccac gacatgatcg actttttcgg tcaggtttgc caggccgttt gaaatgaggg
     8881 tgtaattgat tgggcaactg tgcgccacgc tactttcttc ttcgcttaac gcgataggat
     8941 taactgcttc ataaggcagt tcgatggttg aaacactctg caacaccagg gcatctttat
     9001 tacgcagccc ttcgggtgtc tctttgctcc ccttcgctac gggtttatat cccgcaaccg
     9061 tttttccctg ggaggctaac gcttgtagca atgcgcggga aaccaccgtt ttccctacag
     9121 aagtgtctgt accggtaata aagaaacgct tcagcatcac taactccacc gttatgcttc
     9181 acaaatataa accaggaaaa taattaacct tgaaagtcta agttatgctt tcctggccca
     9241 aattgagata gcgcaaattt tggtagaaca gttaaaaaat gttaaccctg caacagacga
     9301 atcaacaaag aaccgttata catcgcgtct tttaccagcg cagcccctgc catcgtgccc
     9361 tggttagaaa actgagtact ctcaacgcta atgtgctgac tatacgcagg aagggcctgc
     9421 tgacggatgc tatctgagat gaccgggaag aggatatctg ccgctttact taacggtgag
     9481 ccaatcagta ttttttgtgg gttaaataaa ttcaccatga tggcaagaat gcgccctaca
     9541 tgcgcgccca ccccggtaat gatgtctttt gccagcagat cgccgcgcaa tgccgcctga
     9601 cacaatgagt ccacggttaa cggttgccca tgtaacatcg agctcatgga ttgattgaga
     9661 cgcagctgtg ccagctcaag aatactgtcc acactggcga tggtttcgag gcagccgtgg
     9721 ttcccgcaat aacagcgttt accatatggg tctacctgtg tgtgacctat ttccacgagg
     9781 ctactgctgc ctgcgtgtag cagatgacca tcggtaatga cgcccgcccc cacgttgtga
     9841 tcgataacca cctgaatcac atcgcgcgcc ccgcgtgagg caccaaacaa ggcctctgcc
     9901 atcgtccatg cgctgatatc atgctgaata taaaccggaa cgccggtatg ctgctccaga
     9961 gcctcgccga gcggcatctc ttttacatcc tcgtagaacg gcatgcgatg tacaatgcca
    10021 ttttccgtat caataattcc cggcagggtt atggcaatcg aagttagacg ctcaagtttt
    10081 ttctggtggc ggataaaaaa ctgatcgata tgggaaatga tacgatccag caatggcgag
    10141 tcatctttta acgccagttc ctgcgactct tccaccacca gtttgctgct cagatcgcgc
    10201 agagcaagga aaatctcccc gcgactaatg cgcagagaaa gatagtgcca ggcttcagtt
    10261 tcaaccacca gccccaccgc cggacggcca cggttccccg cttctttgat ttccagctct
    10321 tgcaccaggt gtgcttcgag catctcacgg acaattttag tgatactggc aggagccagc
    10381 tgcgccagac gggaaagatc gatacgcgag actggaccaa gctgatcaat caggcgataa
    10441 accgcgcccg cgttggtctg ctttatttga tcaatgtgcc caggctggtt ttcagcaacc
    10501 accgcatact ccctatattt tcgcgctccg aaataatctg taggctatgg tgaagcactt
    10561 caatacgtgt cgtcaaattt ttacttaggc atgtgattaa cagcacattt ttcgggcttt
    10621 ttcgctgaaa tttccctcac ctgagagcat tcagcagatg aatacgaaag ttacgcgcag
    10681 ctggactttg ttcatgatgt ttcggccaga ccaaccacat ttcagaaacc gcatcctctt
    10741 cagcaatcgg cacccagcgc atttcgttga gctgaacacg tttaaatgac gcaggcaaaa
    10801 ttgaaacacc cagaccggcg gaaaccagac cgatgatggt cattgcctcg cccacctcct
    10861 gagtgatgac gggcgtcaaa tggtaacgtc gcatcagccc gagaatatcg tcatacagcc
    10921 ctgtcccgac gtgcggatca aaaaagacaa agggttcttt cgccagttca gccagcgtta
    10981 cattcgggtt atttgccagg ggatgatcgt gcgggatcat cgccataagc ggttcatgga
    11041 cgatgactgc gtgttcaagc gtctccggta acgctgtgtt acgcagcaat cccatatcca
    11101 gcgttccttc aatgagcgga gcgatttgct cgcgagtgtt catttcgcgg gtttgtaaat
    11161 ggacatcggg ataatctcgg cgaaacagcg ataacgtatc ggacaccgcc cgaataaaag
    11221 gagccgacga agtaaaacca atgcgcaact cccccgcttc accctgatgc agtctttcag
    11281 cgcgagcggc agcgtcatcc accatagaca ggatttgccg actatctgca agaaactgtt
    11341 ttcctgctgc cgtcagcaat acactgcgat tggttcgtgc cagcagtcgg gcaccaattt
    11401 gttgctccag cgcctgaatc tgctgactta gcggcggttg cgaaatattc aggcgggcag
    11461 cggcgcgccc gaaatgcagc tcttccgcaa cagcaacaaa gtaacgca
//
LOCUS       DAZFDC010000136        11466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFDC010000136 DAZFDC010000000
VERSION     DAZFDC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.136"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..67
                     /locus_tag="ACHJ48_003942"
     CDS             <1..67
                     /locus_tag="ACHJ48_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709397.1"
                     /translation="REEPIDFLDRSFSSSSWPFYH"
     gene            373..1761
                     /gene="narU"
                     /locus_tag="ACHJ48_003943"
     CDS             373..1761
                     /gene="narU"
                     /locus_tag="ACHJ48_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO4709398.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWKNKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRMKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1843..5583
                     /gene="narZ"
                     /locus_tag="ACHJ48_003944"
     CDS             1843..5583
                     /gene="narZ"
                     /locus_tag="ACHJ48_003944"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO4709399.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWPELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLAGGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLSPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRICPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5580..7124
                     /gene="narH"
                     /locus_tag="ACHJ48_003945"
     CDS             5580..7124
                     /gene="narH"
                     /locus_tag="ACHJ48_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO4709400.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSIAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7124..7819
                     /gene="narW"
                     /locus_tag="ACHJ48_003946"
     CDS             7124..7819
                     /gene="narW"
                     /locus_tag="ACHJ48_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO4709401.1"
                     /translation="MQILKVIGLLMEYPDEMLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7816..8496
                     /gene="narI"
                     /locus_tag="ACHJ48_003947"
     CDS             7816..8496
                     /gene="narI"
                     /locus_tag="ACHJ48_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO4709402.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8575..9468
                     /gene="yddE"
                     /locus_tag="ACHJ48_003948"
     CDS             8575..9468
                     /gene="yddE"
                     /locus_tag="ACHJ48_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO4709403.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDGYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9564..10408)
                     /gene="nhoA"
                     /locus_tag="ACHJ48_003949"
                     /pseudo
     CDS             complement(9564..10408)
                     /gene="nhoA"
                     /locus_tag="ACHJ48_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
     gene            10707..11150
                     /locus_tag="ACHJ48_003950"
     CDS             10707..11150
                     /locus_tag="ACHJ48_003950"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO4709404.1"
                     /translation="MPVEFEPPRVTIVVDKSTWTRELIERNGKFGIVIPGVAATNWTW
                     AVGSVSGREEDKFNCYGIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEEYDTLFG
                     EVVSAAADARVFVEGRWQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11154..11381)
                     /gene="pptA"
                     /locus_tag="ACHJ48_003951"
     CDS             complement(11154..11381)
                     /gene="pptA"
                     /locus_tag="ACHJ48_003951"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO4709405.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
BASE COUNT         2704 a         2974 c         3018 g         2770 t
ORIGIN      
        1 aagagaggaa ccgatagact ttctggacag atcgttttcc tccagctcat ggccttttta
       61 tcactaattc tataagtgtc tggtcttgtt aaaaatatag ttaatataca accctttgga
      121 ttcatcatct gaagggttga atcatgacat gcgtacatag cttaaccccg tttaatggtt
      181 agcttgttag ttacatttag taacacataa ctggctgctt aatccccatt ctgcccccgc
      241 ggttacgctt tcttatctat tctttcaatc agcacttcac ccatttcatc cttaattcta
      301 ataactggct attgcgcctt acctcctgtt ttgtcttttt ttcattacca atgtgtacat
      361 gtgaggaaca atatggcact gcaaaatgag aaaaatagtc gttatctttt acgcgactgg
      421 aaaccagaaa atccggcctt ctggaaaaat aaaggaaaac atattgctcg aagaaacctc
      481 tggatatcag tcagttgtct acttcttgcc ttctgtgtct ggatgctatt tagcgcagtt
      541 accgttaatc tcaataaaat cggttttaat ttcactaccg atcaactctt tttattaacc
      601 gcattaccct ccgtttctgg cgcattatta cgtgtcccct actcctttat ggtgcctata
      661 ttcggtggac gtcgatggac ggtttttagt actgcaatcc tgattattcc ttgcgtctgg
      721 ctcggaattg ccgtgcagaa tccgaatact ccttttggga tatttatcgt tatcgctttg
      781 ctatgcggtt ttgcaggtgc gaactttgct tcgagcatgg gcaatatcag tttcttcttt
      841 ccaaaagcca ggcaaggtag cgctcttggg attaatggcg gattaggaaa cttaggtgta
      901 agtgtaatgc agctggttgc accgctggtc atttttgtac ccgtatttgc ctttctcggc
      961 gtcaatggag taccgcaggc cgacggttcg gtgatgtcgc tggcgaatgc cgcatggatt
     1021 tgggtaccgc tactagcgat tgccacgatc gccgcctggt cagggatgaa tgatatcgcc
     1081 agttcacgcg cgtcaattgc cgaccagctc cctgtcttac aacgcctgca tctctggctg
     1141 ctgagcctgc tttaccttgc caccttcggt tcgtttatcg gtttttctgc gggtttcgcc
     1201 atgctggcaa aaacccagtt cccggatgtg aatattctgc gcctggcgtt ctttggccca
     1261 tttatcggtg ccatcgcgcg gtcggtgggt ggtgctattt ccgataaatt cggcggcgtg
     1321 cgggtgacgt tgatcaactt tatttttatg gcgattttca gcgccctgct gttccttacc
     1381 ttaccgggca caggctccgg taacttcatc gccttttacg ccgtatttat ggggctgttt
     1441 ctgaccgcgg gtctgggaag tggttctact ttccagatga tcgccgtcat ctttcgccag
     1501 ataaccattt atcggatgaa gatgaaaggc ggtagtgatg agcaagctca gagagaagcc
     1561 gtcaccgaaa cggcggcggc tctgggcttt atctcagcca tcggcgcagt gggcggcttt
     1621 tttattccgc aggcatttgg catgtcactc aatatgaccg gctctccagt gggcgcgatg
     1681 aaagtgtttt taatcttcta catcgtttgt gtgctactga cctggctggt ttatggtcgg
     1741 cggaagttca gccaaaaata agagatcgat gcctgatgta caaccgtgtc gcatccgaca
     1801 aggcatcaga tcccaattca ttcctggagc aggagtcatg tcatgagtaa acttttggat
     1861 cgctttcgct acttcaaaca aaagggcgat acctttgccg atggtcacgg acaggtgatg
     1921 catagcaacc gcgactggga ggacagctat cgccagcgtt ggcagttcga caaaatcgtg
     1981 cgttccaccc acggtgttaa ctgtacaggc tcctgtagct ggaaaatcta cgttaaaaat
     2041 ggtctggtga cctgggaaat ccaacagacc gactacccgc gcactcgccc tgacctgccc
     2101 aatcatgaac ctcgcggctg cccgcgtggc gcaagttact cctggtatct ttacagcgct
     2161 aaccgcctga aatacccgct cattcgtaaa cgactgattg aactgtggcg cgaagccctc
     2221 aaacaacaca gcgatccggt actggcgtgg gcatcgatta tgaacgatcc gcaaaagagc
     2281 ctgagctaca aacaagtgcg tgggcgcggc gggtttatcc gctccaactg gccggaacta
     2341 aaccagctga ttgccgccgc taacgtctgg actatcaaaa cctacggccc ggatcgcgtt
     2401 gccggtttct cgccgatccc ggcgatgtcg atggtttctt acgccgccgg aacacgttat
     2461 ctgtcactat taggtggcac ctgtttaagc ttctacgact ggtattgcga cctgccgccc
     2521 gcctcgccga tgacctgggg cgagcaaacc gacgtaccgg aatctgccga ctggtataac
     2581 tccagttaca tcatcgcgtg ggggtctaac gtaccgcaga cacgtacgcc ggacgcccat
     2641 ttctttaccg aagtacgcta caaaggcact aaaaccatcg ccattacccc tgactactct
     2701 gaagtggcca aattgtgcga ccagtggctg gcaccaaaac aaggcactga tagcgccctg
     2761 gcgatggcaa tgggccatgt gattttaaaa gagtttcatc tcgataatcc cagcgactac
     2821 tttatcaact actgccgccg ctatagcgac atgccgatgc tggtaatgct ggaacctcgc
     2881 gacgatggta gctacgtacc cgggcgcatg atccgcgcgt ctgacctggt agatggacta
     2941 ggcgaaagca ataatccgca gtggaaaacc gtggcggtta ataccgcagg tgaattggta
     3001 gtgccgaatg gttcgattgg tttccgctgg ggagaaaaag gcaaatggaa tctggaatcc
     3061 attgccgccg gtaaggaaac cgaattgtcg ttaaccctgc tcggtcaaca tgacgccgtt
     3121 gcaggcgtgg ctttccccta ctttggcggc atcgaaaatc cgcattttcg cagcgtcaaa
     3181 cacaatcctg tcctggtgcg tcaattaccc gttaaaaacc tgacattagc cggtggcagc
     3241 acctgtccag tggtcagcgt ttatgatttg gtactggcga attacggcct cgatcgcggg
     3301 ctggaagatg aaaacagcgc gaaagattac gctgaaatca aaccgtacac cccagcctgg
     3361 ggtgagcaaa ttaccggcgt gccgcgccag tatattgaaa ccatcgctcg tgaatttgcc
     3421 gatactgccc ataaaacgca tgggcgctcg atgattatcc tcggcgcagg tgttaaccac
     3481 tggtatcaca tggacatgaa ctaccgggga atgatcaata tgctgatctt ctgcggttgt
     3541 gtcggacaaa gcggtggcgg ctgggcacac tatgtcggtc aggaaaaact gcgcccgcaa
     3601 accggctggt tgccgctggc ctttgcgctc gactggaacc gaccaccgcg ccagatgaac
     3661 agcacctcgt ttttctacaa tcattccagc caatggcgct atgaaaaagt ctctgcgcag
     3721 gagttacttt caccgcttgc cgatgccagt aagtacagcg gtcatctgat tgattttaac
     3781 gttcgcgccg aacgcatggg ctggctacct tctgcgccgc agttggggcg taacccgctc
     3841 gggattaaag ctgaagccga caaggccgga ttatccccca cagaatttac cgcccaggcg
     3901 ctgaaatcgg gcgatttacg tatggcctgc gaacaaccag atagcggcag caatcatccg
     3961 cgtaatttgt ttgtctggcg ttctaacctg cttggctcct ccggcaaagg ccacgagtat
     4021 atgcagaagt atctgctggg gaccgaaagc gggattcagg gcgaggaact cggtgccagc
     4081 gacgggatca aaccggaaga agtcgagtgg caaactgcgg cgattgaagg caagctcgac
     4141 ctgctggtga cgctcgactt ccgcatgtcc agtacctgcc tgttctccga tatcgttctg
     4201 cccaccgcca cctggtatga aaaagacgat atgaacacct cggatatgca tccatttatt
     4261 catccgcttt ctgcggcggt cgatccggcc tgggaatcac gcagcgactg ggaaatctac
     4321 aaaggtattg ccaaagcatt ttcgcaagtg tgcgtgggcc atcttggcaa agaaaccgac
     4381 gtggtattac aaccactgct gcatgactct ccggcagagc tctcacagcc gtgtgaagtg
     4441 ctcgactggc gcaaaggcga atgcgatctg agcccgggta aaaccgcgcc gaatattgtg
     4501 gcggtggagc gcgactaccc tgctacgtat gaacgcttta cctcgctcgg gccattgatg
     4561 gacaaacttg gcaacggcgg taaagggatt tcgtggaata cgcaggatga aatcgatttc
     4621 ctcggtaaac tcaattacac caagcgtgat ggcccagcgc aggggcgtcc gctgattgac
     4681 accgccattg acgcttcaga agtgattctg gcactggcac cagaaaccaa cggtcatgtt
     4741 gcagttaaag cgtggcaggc gctgggcgag atcaccggac gcgaacatac ccatctggcg
     4801 ctgcacaaag aggacgagaa gattcgcttt cgcgatattc aggcgcagcc gcgtaaaatt
     4861 atctccagcc ccacatggtc tggtctggaa agcgatcacg tctcctataa tgcgggatac
     4921 accaacgttc atgagttaat tccgtggcgc acgctgtcgg gacgccagca gctctatcag
     4981 gatcatccgt ggatgcgtgc ttttggtgaa agcctggtgg catatcgccc gcctatcgac
     5041 acccgtagcg tcagtgagat gcgccagatc ccgccaaacg gcttcccgga aaaagcactt
     5101 aacttcctga cgccgcacca gaaatggggc attcactcaa cctacagtga aaacctgcta
     5161 atgctgacgc tctctcgcgg tggaccgatt gtctggatca gcgaaaccga tgcccgtgaa
     5221 ctaaccattg tcgataacga ctgggtggaa gtgtttaacg ccaatggcgc gctgacggcc
     5281 cgcgcggtgg tcagccaacg tgtaccgccg ggtatgacca tgatgtatca cgctcaggaa
     5341 cgcattatga atattcctgg ttcggaagta actggcatgc gcggcggtat tcataactcg
     5401 gtcacccgca tttgcccgaa accaacgcat atgattggcg gttacgcgca gctggcctgg
     5461 ggctttaact actacggcac cgtcggctcg aaccgcgacg aattcatcat gatccgcaag
     5521 atgaagaacg ttaactggct ggatgatgaa ggtcgcgatc aggtacagga ggcgaaaaaa
     5581 tgaaaatccg ttcgcaagtc ggcatggtgc ttaatctcga taaatgtatc ggctgccata
     5641 cctgttcggt gacctgtaaa aacgtctgga ccggacgcga aggcatggag tacgcatggt
     5701 ttaacaacgt cgaaaccaaa ccgggcattg gttatccgaa aaactgggaa gatcaggaag
     5761 agtggcaagg cggctgggtc cgcgatgtga atggcaagat acgcccgcgt ctgggcagca
     5821 agatgggtgt aataaccaaa atcttcgcca acccggtggt gccgcagatt gatgattact
     5881 acgaaccttt caccttcgac tacgaacatt tgcatagcgc accggaaggc aaacatatcc
     5941 ctactgctcg cccgcgttcg ctgattgacg gtaagcggat ggacaaagtg atctgggggc
     6001 caaactggga agaactgctg ggcggcgagt ttgaaaaacg tgcccgcgac cgcaacttcg
     6061 aggccatgca aaaggagatg tacgggcagt ttgaaaacac cttcatgatg tacctgccgc
     6121 gcctgtgcga acactgcctc aatcccagct gcgtggcgac ctgcccaagc ggcgctatct
     6181 acaaacgcga agaagacggc attgtgctga ttgatcagga taaatgccgt ggctggcgtt
     6241 tgtgcattag cggttgtccg tacaaaaaaa tctacttcaa ctggaaaagc ggcaagtcag
     6301 aaaaatgtat cttctgttac ccacgaattg agtccggtca accgaccgtg tgctcagaaa
     6361 cctgcgtggg tcgcatccga tatctgggcg tgctgcttta cgacgctgac cgcatcgagg
     6421 aagcggcgag caccgagcgc gaggttgacc tctatgaacg ccagtgcgaa gtgttcctcg
     6481 atccgcacga tccctcagtg atcgaggaag ccctgaaaca aggtattcca caaaatgtga
     6541 ttgacgctgc ccagcgttcg ccagtctaca aaatggcgat ggactggaaa ctggcactac
     6601 cgctgcaccc tgaataccgc accctgccga tggtctggta cgttcctccg ctgtcaccga
     6661 ttcagtccta cgcagatgcg ggcggtttgc cgaaaagcga aggcgtgctg cccgccatcg
     6721 aaagcctgcg tattccggtg caatatctcg ccaatatgtt gagtgccggc gataccggtc
     6781 cggtactgcg ggcgctgaaa cggatgatgg cgatgcgcca ctatatgcgt tcacaaaccg
     6841 tggaaggcgt tactgatact cgtgccatcg acgaagtagg cctgagcatc gcccaggtcg
     6901 aagaaatgta tcgctatctc gccattgcca actatgaaga tcgttttgtt atcccgacca
     6961 gccatcggga aatggcgggc gatgccttcg cagaacgcaa tggctgcggt tttacctttg
     7021 gtgacggttg tcacggctcg gacagcaaat tcaacctgtt caacagtagc cgtatcgatg
     7081 ccatcaacat caccgaagtg cgcgacaaag cggagggcga ataatgcaga tcctcaaagt
     7141 gatcggcctg ttgatggagt atccggacga gatgttgtgg gaatgcaagg aggacgcgct
     7201 ggcgttgatc cgccgcgatg cgccgatgct tacggatttc acccgcaacc tgcttaacgc
     7261 gccgctgctg gataaacagg ccgaatggtg cgaagtgttt gaccgcgggc gcaccacgtc
     7321 gctgctgctg ttcgaacatg ttcatgccga gtcccgcgat cgcggccagg caatggtgga
     7381 cctgctggcg gagtatgaaa aggtcggcct gcagctggat tgtcgggaac tgcccgatta
     7441 tctaccgctg tatctggagt atttaagcgt actgcctgac gatcaggcga aagaaggatt
     7501 gctcaacgtt gcgccgatcc tcgccctgct tggcggtcgc ttaaaacaac gcgaggcacc
     7561 gtggtacgcg ttgtttgatg ctctgctgca actggcggga agtacacttt caagtgacag
     7621 tgtcacaaaa caagtaaaca gcgaagagcg cgatgacacc cgccaggcgc ttgatgcagt
     7681 gtgggaagag gaacaggtga agtttattga agataacgcc acggcgtgtg acagctcgcc
     7741 gcttaatcaa tatcagcgac gctttagtca ggatgtcgcg ccgcaatatg tcgacatcag
     7801 tgcgggaggt gggaaatgat tcagtatctg aacgtctttt tttacgatat ctacccgtac
     7861 atttgtgcga cggtgttttt cctcggcagc tggctgcgct acgactacgg gcagtacacc
     7921 tggcgcgcct cctcaagtca gatgctcgat aaacgcggga tggtgatatg gtcgaatctg
     7981 ttccatatcg gcattttggg gatcttcttc gggcacctgt tcggcatgtt aacgccacac
     8041 tggatgtacg cgtggttttt gccagtggca gcgaaacaac taatggcgat ggtgctcggt
     8101 ggtatttgcg gtgtgttgac gttgattggc ggcgcaggac tgctgtggcg caggctgaca
     8161 aatcagcgcg tgcgtgccac ttccactacg ccggatatca tcatcatgag cattttgctg
     8221 atccagtgtc tgttggggct aagtaccata ccgttctcag cacagtatcc tgatggtagc
     8281 gaaatgatga agctggtggg ttgggcgcaa agcattgtca ctttccgtgg tggatcgtca
     8341 gagatgctta acggtgtagc gttcgtcttc cgcctgcatc tggtgttggg aatgaccatc
     8401 ttcttgctct tcccattcac ccgactggtg catgtgtgga gcgccccgtt tgagtacttt
     8461 actcgtcggt atcagattgt gcgctcgcgg cgataatttc accctggccg gatagccctt
     8521 cgctctccgg ccacttgcag gctatgatag ttctccagtc acaggagaac gttaatgaaa
     8581 ccgcaggttt accacgtcga tgcttttacc tctcaaccat ttcgcggcaa ttctgccggt
     8641 gttgttttcc ccgccgataa tcttagcgaa gcgcaaatgc agcttatcgc ccgcgaatta
     8701 ggtcattcgg aaaccgcttt tctgctgcac agcgacgaca gcgatgtgcg catccgctac
     8761 tttacgccaa cagttgaagt gcctatttgc ggtcacgcga cagtagctgc gcactatgta
     8821 cgtgcgaagg tgttaggttt aggaaattgc acggtctggc aaacatcgct ggcaggaaaa
     8881 catcgcgtga ctatcgaaaa gcaccacgat ggttatcgta tttcgctgga acaaggtacg
     8941 ccaggctttg agccaccgct ggaaggtgaa acacgtgcgg cgattatcaa cgcgcttcat
     9001 ctcactgagg acgatattct gccaggcttg ccaattcagg tggcaaccac aggacactca
     9061 aaagtgatga tcccactgaa accggaagtg gatatcgacg ccctttcgcc tgaccttaat
     9121 gcgctgaccg ctatcagcaa acagattggc tgcaatggtt tcttcccgtt ccagatccgc
     9181 ccaggcaaaa acgaaaccga tggtcgcatg ttctcgcctg cgattggtat tgtggaagat
     9241 ccggtgactg gcaatgccaa tggcccgatg ggtgcatggt tggtacatca caacgtattg
     9301 ccccacgatg gcaacgtgtt gcgtgttaaa ggccatcagg ggcgcgcatt ggggcgtgac
     9361 ggtatgattg aagtgacggt gacaattcgt gataaccaac cggaaaaagt caccatttct
     9421 ggcgcggcgg tgattttgtt tcatgccgaa tgggcaatta aactctgaaa ccgttgcctg
     9481 atgcgcttcg cctgtcaggt ctacaaccgg gctaatgtat gccggataag gcgtttacgc
     9541 cgcatccggc aacctgacat aaattatttt cccgcctccg ggtgcgtatc aaacgccgcc
     9601 atcaccagcg ctaactcatc cacggtaaag ccatgtttcg catcatccac gcccagacca
     9661 aactgttctt gcatcacagc atataacgac gccacatccg gtagatttcg ctgctccacc
     9721 gcgtgcccat tttcataatg ggaaaatgaa aattggtcag tgtcagcttg ccgccgtccg
     9781 gcaaatggcg gcacatcagc aaatgatggc gaaaatgcga ctgcggccag tgcgccgacc
     9841 agaaattacc catcacataa tcgctttgtt gctgctcgca gagatcaaaa cggtacatcg
     9901 actgccagtg ctgatgatga ttaaactgca acacccaatc atcaccctcc tgcaacaacc
     9961 gatactctcc gtgtggcgtg gtctgcacaa gatcgggaac taaacgaatc ggcgcggtta
    10021 gcgtctgccc accgaaaccg acatcagcaa tccatttttc ctcttccagt tccaccaaca
    10081 gcaaacgatg ggtgcgcggc ggtaatgctg gcggatttga taacactacc cgccctaaca
    10141 agctgcgaac gttaaacccc agctcgcgta acacccgctc aaaaacgcca ttctgctcaa
    10201 aacagtaacc gccacgacgg gctatcacca gtttctcttc cagcgattga tcatcaagct
    10261 gcatttccct cggcagcaaa acgtcgaggt tttcaaacgg aatggtgcaa ttgtgtttca
    10321 ggtgcaatgc acgaagcgta tcaatattga ccgcagcagc tcccgaccag ttaatacggg
    10381 caaaatagtg attcagaatg ggcgtcatca ggcgtttccc ttagtggttt tctcaactta
    10441 taacctggaa tagacgccgc accggctatt ttgtgcgatc gcggacatgc atcacgttcg
    10501 caaatcgcca atcccgttaa tttcgttact tattgcgtta cggactacgc ttaaaaaaac
    10561 cttcttcgcg aggcgatacc atgagccgat tcatccccat agaattacac catgccagcc
    10621 ggctgttaaa tcatggccca actgtcatga tcaccagttt cgatgagcaa tcccagcggc
    10681 gtaacattat ggctgcagcc tgatcaatgc cggtggagtt tgaacctcca cgcgtgacga
    10741 ttgtggtaga taaatcgaca tggaccaggg agttgattga acgtaacggt aaatttggca
    10801 tcgttatccc gggcgttgca gcaactaact ggacgtgggc agtgggaagt gtgtcgggtc
    10861 gtgaagaaga taaatttaat tgctatggca ttccggttgt gagaggcccg gtatttggtt
    10921 tgcctctggt cgaggaaaaa tgtctggcgt ggatggagtg tcgattgcta cctgcgactt
    10981 ctgcgcaaga agaatacgac acgctgtttg gcgaagtagt atcagcagcg gcagacgcac
    11041 gggtatttgt cgaaggccgc tggcagtttg atgatgataa gctcaatacg ttgcatcatt
    11101 taggtgctgg gacgtttgtt accagcggca agcgtgttac ggcgggttaa gcattacatg
    11161 ctgtaaccgg gtttctttat caaagcctcc atttggggcg cgatttcggc atcccagata
    11221 gcttgccaag attctggttg aatctgttgt agagcaatgc ttatcgaact gtctttactg
    11281 ttcagatggc gaataataac gtcggtaata tctgcagcaa gtgctgcttt ttgttgttcg
    11341 tccagttcac gcggaaaaca tttaatgtct atgtgcggca tgattacgct ccttttgaaa
    11401 gatgtcagta aaattaacac acattgtgaa tgtcgtgtgt ccggtaaagc gtttttacga
    11461 caataa
//
LOCUS       DAZFDC010000137        11439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFDC010000137 DAZFDC010000000
VERSION     DAZFDC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.137"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            290..892
                     /gene="lpfA2"
                     /locus_tag="ACHJ48_003952"
     CDS             290..892
                     /gene="lpfA2"
                     /locus_tag="ACHJ48_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFO4709406.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
     gene            1053..1379
                     /locus_tag="ACHJ48_003953"
     CDS             1053..1379
                     /locus_tag="ACHJ48_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4709407.1"
                     /translation="MATAGIVVGGTRVIFYGDKPDTTISIFNKETNLPYLLQTWVDPF
                     NKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRESVFWLNVKNIPPSIDKK
                     IPMFLR"
     gene            1532..1681
                     /locus_tag="ACHJ48_003954"
     CDS             1532..1681
                     /locus_tag="ACHJ48_003954"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4709408.1"
                     /translation="MDDKDVSLNMIRPFEILTLPIPAGVAGKSLVWRFINDYGAISQP
                     LKKNL"
     gene            1703..4237
                     /locus_tag="ACHJ48_003955"
     CDS             1703..4237
                     /locus_tag="ACHJ48_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4709409.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHPADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFAQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYDDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSMEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSLGNHHDDNNDDFGKEQIF
                     MFSMSIPLSCWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLVQMDAICR"
     gene            4249..5319
                     /locus_tag="ACHJ48_003956"
     CDS             4249..5319
                     /locus_tag="ACHJ48_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4709410.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGATHMSNLSFGPLTVAAANNH
                     SGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLERTLNGLN
                     YYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNLDSGRSAK
                     LSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPQTTPVSKVTIRGQLTAPQNCELTPNQ
                     SIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASMIAANRSA
                     DATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFYSAPASLT
                     GAKPAPDNGFTATATLVIEFTN"
     gene            5347..6429
                     /locus_tag="ACHJ48_003957"
     CDS             5347..6429
                     /locus_tag="ACHJ48_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4709411.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGYERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            6641..7681
                     /gene="pstS"
                     /locus_tag="ACHJ48_003958"
     CDS             6641..7681
                     /gene="pstS"
                     /locus_tag="ACHJ48_003958"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO4709412.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            7768..8727
                     /gene="pstC"
                     /locus_tag="ACHJ48_003959"
     CDS             7768..8727
                     /gene="pstC"
                     /locus_tag="ACHJ48_003959"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO4709413.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            8727..9617
                     /gene="pstA"
                     /locus_tag="ACHJ48_003960"
     CDS             8727..9617
                     /gene="pstA"
                     /locus_tag="ACHJ48_003960"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO4709414.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            9800..10573
                     /gene="pstB"
                     /locus_tag="ACHJ48_003961"
     CDS             9800..10573
                     /gene="pstB"
                     /locus_tag="ACHJ48_003961"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO4709415.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            10588..11313
                     /gene="phoU"
                     /locus_tag="ACHJ48_003962"
     CDS             10588..11313
                     /gene="phoU"
                     /locus_tag="ACHJ48_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO4709416.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         2966 a         2674 c         2836 g         2963 t
ORIGIN      
        1 aaaattgtct gctttgagat gtgtgatata attaaaaaat cacctcatca taaaaataat
       61 atttatatcc atttattatt ccctcttctt ttaagcaatc acataacgat ataaatttag
      121 ttttgcttaa cacctaaatt aatttatcta aatgtataaa ataaaaacca cttgcgccaa
      181 agtatattgg tagtactatt tggtttctga tcaaataccg atcaggttta tccaaattta
      241 ataaatggat aacacacagg aataatatca aattaatgga gttatattta tgaaaccaaa
      301 tatgattgta ggagcattag cgttaacttc tgtgtttatg gcaggtcacc tacaggcggc
      361 tgatggaaca gtccatttcc gtggtgaaat tattgacagt acttgcgaag tcactcctga
      421 aactaaagat caggtcgttg atttaggcaa agtaaaccgt acagccttta gtggcgtcga
      481 tgatgtggct gccccgacgg ctttttctat cgatctgact caatgcccgg aaacctttaa
      541 gtccgccgca attcgtttcg atggtaatga agatgctcat ggtaatggca acctggcaat
      601 tggtaccccg ctggataact ctaacgatgc tgccgctggt attagcccga gtgataacag
      661 tggggattat actggtgcgg gtgccgttag tgcagcgaaa ggcgtagcta ttcgtttata
      721 taaccgtgca gataacactc aggtcaagtt atatgaaaat tctgcatcaa ctccgatttc
      781 taatggtaat gcatccatga agttcatggc tcgttatatt gctacggaaa cgactattga
      841 ccctggtaca gctaacgccg actcgcagtt tacagttgaa tatataaaat aaatcattct
      901 taacataata ccttaaataa aatggcgctc gccattatgg ttctctttta cttaaatgaa
      961 gggcgtcatt attatatttt ttatgagata catttatgcc tatgtattta cttagattgt
     1021 tttttgctgt tactttgatt acgcagatgt cgatggctac ggctggtatt gttgttgggg
     1081 gaactcgcgt tattttttat ggtgacaaac ctgataccac aatctctatt tttaataaag
     1141 aaacgaacct tccttatcta ttgcagacat gggttgatcc atttaataaa gaggataaaa
     1201 gcaaaccgcc attttctgtg atacctcccg tttctcgcat tgaaccacag caagagaaaa
     1261 tattacgcat tttaaaaacg gctggcgagt taccaaaaga tcgggagtcc gtattctggt
     1321 tgaacgtgaa aaacatccct ccttcaattg ataaaaaaat accaatgttc ttgagatagc
     1381 cataaaaaca agaattaagt tattctggcg accagccgcg gtaaagataa tccctgagca
     1441 ggctattaaa aatgttaaat ggtcgcggca gggtaataat ctcatcgtcg aaaatccaaa
     1501 tccgatccat attaatgtaa tgagtgtgac ggtggatgat aaagacgtgt cgctaaatat
     1561 gatccgccct tttgaaatat tgacattacc aattccggct ggcgtggcag gaaaatcgct
     1621 cgtttggcgc tttattaatg attacggtgc aattagtcaa ccactaaaga aaaaccttta
     1681 agcgccggat aaggatatat cgatggcctc ttcgcatctt ttcattacgc ttgcatcggg
     1741 catatgtctg ctctgttcca tatctgcttt tgcccgggat agcttgttca accccagatt
     1801 actggaactg gatcatcctg cggataatat tgatattcac cagttcaacc gttcgaatac
     1861 cttacctgcg ggaacataca aagttgatgt gatgatcaac ggcatgctct tcgaacgcca
     1921 ggaagttaaa ttcgcccagg ataaccctga tgctgaactc cacccatgct acgtggcgat
     1981 aaaaaacgtg ctggcgacct atggtataaa agttgatgcg ataaaatctc tggcgaatgt
     2041 tgatgacaaa acatgcgtaa atccagttcc gctgatcgac ggggctacct ggttactgga
     2101 cgccagtaaa cttgcattga atattactat tccgcaaatt tatctcaaca atgcagttaa
     2161 tggttatatc agcccttccc gttgggatca ggggatcaat gccatgatga tgaattatga
     2221 tttttcggca tcgcatacca tccggtcaaa ttatgacgac gacgatgaca gttattatct
     2281 gaatttgcgt aatggtatta atttaggcgc atggcgtttt cgtaattaca gcaccctgaa
     2341 ttcttatgac ggtaatgtgg actaccattc cgtcagtaat tacattcagc gcgacatcat
     2401 ggcattacgt agccagatta tgattggcga tacctggacg gcaagcgatg tatttgatag
     2461 tacacaggtg cgtggcgtgc ggctgtatac cgatgacgat atgttgccct ccagccagaa
     2521 cggctttgcg ccagtggtac atgggattgc gaaaactaac gccacggtga tcatcaaaca
     2581 aaacggctac gttatttatc aatcagccgt accacagggc gcatttgccc tcaccgactt
     2641 aaacacgacc agtagcggcg gcgatctcga tgtcactatc aaagaagaag atggcagcga
     2701 gcagcacttt attcagccat ttacttcact ggccattctc aagcgtgaag gtcagaccga
     2761 tgtagacctt agcattggag aagtgcgcga cgaaagcggc tttacgcctg aggtcttgca
     2821 gttacaagca atgcacggtt tccctttggg aataactttg tatggcggaa cacaattggc
     2881 aaatgattac gcttctgccg cgctgggtat tggtaaagat atgggggcgc tgggcgcgat
     2941 ttcttttgac gtgactcatg cccgctcgca gtttgactac gacgataatg agagtggtca
     3001 atcgtatcgt tttctctatt ccaaacgttt tgaagacacc aataccacct ttcgtctggt
     3061 gggttatcgc tactctatgg aggggttcta caccctcaat gaatgggtgt cgcgacagga
     3121 taatgattct gatttctggg taacgggcaa ccgtcgcagc cgcttcgaag gcacctggac
     3181 gcaatctttc acgccaggct ggggcaatat ttatttaaca ttcagtcgac aggaatactg
     3241 gcagaccgat gaggtcgaac gtttattaca gttcggctat aacaacaact ggcgaaacat
     3301 ctcctggaac gtttcctgga actatacgga ctcgatcaag cgctcattgg gcaaccatca
     3361 tgatgataac aatgatgatt tcggcaaaga acagattttc atgttctcaa tgtcgatacc
     3421 gctatcgtgc tggatggaag acagctacgt caactattcg ttaacgcaaa acaaccacca
     3481 tgaaagcacg atgcaggtcg gtctgaacgg aacgatgctg gaagggcgta acctgtctta
     3541 taacgtacag gaatcgtgga tgcactctcc tgatgactcc tacagcggca atgccggaat
     3601 gacctatgac gggacttatg gctcggtcaa tggtagctat tcctggagcc gtgactccca
     3661 acattttgat tatggcgcca gaggcggcgt gctggtgcat agtgacggag tgaccttctc
     3721 gcaggaactg ggcgaaacgg tggcattggt caaagcgccg ggcgcagaag gcctgtccat
     3781 tgaaaacgcc accgggattt ctaccgactg gcgtggttat accgtaaaaa cgcagcttag
     3841 cccgtatgac gaaaaccgcg tggcattgaa cagcgactat ttctccaaag ccaatattga
     3901 actggaaaac accgtcatca acctggtacc aacgcgcggt gcggtggtga aagccgaatt
     3961 tgtcacccat gtcggttatc gcgtgctatt taacgtccgc caggtcaacg gtaaaccaat
     4021 aatgtttggc gcgatggcaa ccgcctctct cgaaacgggc acagtcaccg ggattgtcgg
     4081 tgataacggc gaactgtatc tctccgggat gcctgaaaaa ggcgagtttt tattgagttg
     4141 gggacaagct gcggatgaaa aatgtaaggc ggcctatcac atcacccata aacctgatga
     4201 taccagcctg gttcaaatgg atgcgatttg tcgctaaagg aagcatgaat gaaaaaaata
     4261 ttatcagggt tgattctgct gctttgctgt ccttatggtt tcgccgctaa tggtgatggc
     4321 gcaacgcaca tgtcaaattt atcatttggt ccgctgacgg tggcagcggc gaataaccac
     4381 tccggataca atattttcga ggcactgagc aacacgactg gaacataccc ggtgcgctgt
     4441 cactgtgatg acacgcatgg cggaccgggc caacaaacag cattttttcc tatcttctac
     4501 acgggagatg ccgcaccggg gcttgtgctt gagcgcactc ttaatgggtt aaattactat
     4561 gctctgaatg attatttatc ggtcggcgtg acgattttta ttattaataa ccaatatgcg
     4621 gccattcctt ttgaacactt atccaaccaa tccacctcac cgcaacatac ctgtggagca
     4681 ggtaataatg ggagcactgt aaatctggat tcagggcgct cggcaaaatt atctttctat
     4741 gttcggcatt ctattactgg cacggtgaca atacccacaa cggaagtcgc ctggttgtac
     4801 gcgggcatgt ccgatcattt tccccaaacg acccccgttt ctaaagtgac aattcgcgga
     4861 caactaacgg ccccgcagaa ctgtgagtta acgccaaatc agagcatcga tgtcgatttt
     4921 caaaaaatta atagcgctga gttctcctca acggcgggtt caattattgc agaaagaaag
     4981 attaaaaccg aagtcacagt atcctgtacc gggatggaag acgtaaggtc cacggaggtg
     5041 gtgagtgcgt cgatgattgc ggcaaacaga agtgccgatg ccaccatgat cgtgacgagt
     5101 aatccggatg tgggaattaa gatttttgat aagaacgacc gtccagtgaa tgtggatggg
     5161 ggcaacttac ccgctgatat gggtgctatt agtcgattag gaaaaaccga tggtagcgta
     5221 acgttttatt cagcgcccgc cagtctgacg ggcgcaaaac cagcgcctga taatggattt
     5281 accgctacag ccacgctggt tattgaattt actaactaat cttgtgttag taaaaggaat
     5341 aatttcatga aaaaatggca ttatatattt tgcataattc tctttcattt agggttaccg
     5401 tgcgggtatg cggcaaatga tggcacgtgt gcaacaagag gcggcacaca tacattaagc
     5461 cttaattttc ctctgacaac ggtcagtgca gcaaacaatg tgcctggaaa tacattaata
     5521 gatattgcta atgcaacatc ttctgaaaat tatagcgttc tgtgtaactg tgattcaaaa
     5581 catagcaatg gcgcttatca cgaaatatat tataccgcag accctgctcc cggtatggtt
     5641 tatagcacca ccgcaagtgg tcttgctttt tactatctta acgaatatgt cgatgtggga
     5701 acaaaaatat ctgtgctaaa tgcggggtat acggcagttc cttttgaaca tgtttccaac
     5761 caggcaacta caacagatca cacttgtcag ggaaacaaaa ctacagcggt tggcgtgagc
     5821 ctgaaaactg gagcagatgc gaagatttca tttcgtatta aacgttcaat aaatggaacg
     5881 gtagtaatac ctatcaccga tattgcattg ctgtatgcca acatatccag caccacgacc
     5941 cgtggtgagg cgattgcaaa agttcgaatt tcaggcagtt tgaccgcacc acagtcttgt
     6001 cagataaatg caggacaggt gatttatttt gattttgata ctattcctgc gtccgaattt
     6061 tcatctaccg ccgggcaagc cattacttca cgaaaaatca ctaaaacagt gagtattgag
     6121 tgtacgggga tggggtatga gcgtacgcag aaagtcgatg cttcttttac ggggacgaac
     6181 cgaagcagtg acgatacgat ggtggcgaca gacaatgctg atgtcgggat caaaatttac
     6241 aataaatcga atgctgaagt tagcgtcaac aacggcaagt tacccgcaga catgggcaac
     6301 acgaccattt ttggtcgtaa aaatggttcg gtaacttttt cggcagcacc tgccagcttt
     6361 accggtgccc ggcctcagcc cggcgttttt aacgctaccg cgaccttaac cattgaattt
     6421 gtaaactaac cttttgcggc tgttttatga cagccgttca tatttcctct cctttcatga
     6481 aaaaatgctg atatttttcc ccttcaatat caataagtaa taactttcct ttgggtgtca
     6541 tcaaactgtc acattcctta catataactg tcacctgttt gtcctatttt gcttctcgta
     6601 gccaacaaac aatgctttat gaatcctccc aggagacatt atgaaagtta tgcgtaccac
     6661 cgtcgcaact gttgtcgccg cgaccttatc gatgagtgct ttctctgtgt ttgcagaagc
     6721 aagcctgaca ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat gggctgacac
     6781 ttaccagaaa gaaaccggta ataaagttaa ttaccagggt atcggttctt ccggtggcgt
     6841 aaaacagatt atcgccaata ccgttgattt tggtgcttct gacgcgccgc tgtctgacga
     6901 aaaactggct caggaaggtc tgttccagtt cccgaccgtg attggcggcg tggtgctggc
     6961 ggttaacatt ccaggtctga agtctggtga gctggtgctg gatggtaaaa ccctcggcga
     7021 tatctacctg ggcaaaatca agaagtggga tgatgaagcc atcgccaaat tgaatccggg
     7081 tctgaaactg ccttcacaga acattgctgt agtgcgccgc gcagatggct ccgggacttc
     7141 cttcgtcttc accagctacc tggcgaaagt gaatgaagag tggaaaaaca acgttggtac
     7201 tggctctacc gtaaaatggc cgatcggtct gggtggtaaa ggtaacgacg gtatcgctgc
     7261 gttcgttcag cgtctgccgg gtgcaattgg ttacgttgaa tatgcttatg cgaagcaaaa
     7321 caacctggcg tacaccaaac tgatctccgc tgatggtaaa cctgttttgc cgaccgaaga
     7381 aaacttcgct aatgcagcaa aaggtgcaga ctggagcaaa accttcgctc aggatctgac
     7441 caaccagaaa ggcgaagatg cgtggcctat tacctctacc acgttcattc tgatccacaa
     7501 agatcagaag aaaccagaac aaggcacaga agtgctgaaa ttcttcgact gggcgtacaa
     7561 aaccggggct aaacaggcga acgacctgga ttacgccagc ctgccggata gcgtagttga
     7621 acaggttcgc gctgcgtgga agaccaatat taaagacagt agcggcaagc cgctgtacta
     7681 ataaaactcc aggccggata aggcattaaa cggcttatcc ggcattacaa aatgactttg
     7741 taaacgcgtt taactgaaga gtaacttatg gctgcaacca agcctgcttt taacccaccg
     7801 ggtaaaaagg gcgacataat tttcagcgtg ctggtaaaac tggcggcgct gattgtgcta
     7861 ttgatgttgg gtggcattat tgtctctctg atcatctcct catggccgag cattcagaaa
     7921 tttggtctgg ctttcctgtg gaccaaagag tgggatgcgc cgaacgatat ctacggggcg
     7981 ctggtgccga tctacggtac gttggtgact tcgtttatcg cgctgctgat cgccgtcccg
     8041 gtgagtttcg gtatcgccct gttcctgact gagcttgcgc ctggctggct gaaacgcccg
     8101 ctgggtatcg ccattgagct gctggcagcc attccaagta tcgtttacgg catgtggggc
     8161 ctgtttatct ttgcgccgct gttcgccgtt tacttccagg agccggtcgg caatatcatg
     8221 tcgaatatcc cgattgtcgg cgcgctgttc tctggcccgg catttggtat cggtatcctc
     8281 gcggcaggcg tgatcctcgc catcatgatt attccgtaca ttgcggcggt aatgcgcgat
     8341 gtgttcgaac aaaccccggt gatgatgaaa gagtcggcct acggtattgg ctgcaccacc
     8401 tgggaggtta tctggcgtat cgttcttccg ttcaccaaaa atggtgttat cggcggcatt
     8461 atgctggggc tgggccgcgc gcttggtgaa accatggcgg tgacctttat tatcggtaac
     8521 acctaccagc tcgacagcgc ctcgctgtac atgccgggca acagtatcac ctctgcgctg
     8581 gcgaacgaat ttgccgaagc ggaatccggt ctgcacgttg ccgcactgat ggaactgggc
     8641 ctgatcctgt ttgtgattac cttcatcgtc ctcgccgcat cgaagtttat gattatgcgc
     8701 ctggctaaga atgagggggc tcgctaatgg ctatggttga aatgcaaacc actgcggcgc
     8761 tggcggaatc ccgccgcaaa atgcaggcgc gtcgccgcct caaaaaccgt attgcactga
     8821 cgctctcaat ggcgacgatg gccttcggtc tgttctggct gatctggatt ttaatgtcca
     8881 ccatcacccg cggtatcgac ggtatgtcgc tggcgctgtt cactgaaatg acgccgccgc
     8941 ccaatacgga aggtggtggt ctggcgaacg ctctggcagg tagcgggctg ttaattttgt
     9001 gggccacggt attcggtacg ccgctgggca ttatggcggg gatttatctg gctgaatatg
     9061 gtcgtaaatc ctggctggca gaagtgattc gcttcattaa cgacattctg ctctctgcgc
     9121 cgtcgattgt ggttggtctg tttgtttaca ccatcgtggt ggcgcagatg gagcacttct
     9181 ccggttgggc gggtgtgatt gcgctggcgt tgttgcaggt gccgattgtt atccgcacca
     9241 ccgagaacat gctgaaactg gtgccggaca gcctgcgtga agcggcttat gcgctgggta
     9301 caccgaagtg gaagatgatc tccgcgatta cgctgaaagc gtcggtgtcc gggatcatga
     9361 ccggtatcct gctggcgatt gcccgtattg ctggtgaaac cgcgccgctg ctgtttaccg
     9421 cgctctccaa ccagttctgg agcacggaca tgatgcagcc gatcgccaac ctgccggtga
     9481 cgatctttaa gtttgcgatg agcccgtttg cggaatggca gcaactggcc tgggccgggg
     9541 tattgatcat taccctgtgc gtactgctgc tgaacattct ggcgcgcgtt gttttcgcga
     9601 agaataaaca cggttgatat tgctgacacg gttttcccct caccctaacc ctctccccag
     9661 aggggcgagg ggaccgaccg agcgcctttt tgactctgta cacggttaac actttgccgg
     9721 atgcggcgtg aacgcctgat ccggcctacg gtaagcctga ttcgcgaagt gcatcaggca
     9781 cgatgaggaa aagattgcaa tgagtatggt tgaaactgcc ccgagtaaaa ttcaggttcg
     9841 taatttgaac ttctactacg gcaaattcca tgccctgaaa aacatcaacc tggatatcgc
     9901 caaaaaccag gtaacggcgt ttatcgggcc gtccggctgc ggtaaatcga cgctgctgcg
     9961 taccttcaac aaaatgtttg aactgtaccc ggaacagcgt gcggaaggtg aaattctgct
    10021 cgatggcgac aacatcctga ccaactctca ggatatcgcc ctgctgcgtg cgaaagtggg
    10081 catggtgttc cagaaaccga cgccgttccc gatgtccatc tacgacaaca tcgcctttgg
    10141 cgttcgtctg tttgagaagc tctctcgtgc cgacatggac gagcgcgtgc agtgggcatt
    10201 gaccaaagcc gcattgtgga acgaaaccaa agataaattg catcagagcg gttactctct
    10261 ctctggtggt cagcaacagc gtctgtgcat tgcgcgtggt atcgccattc gcccggaagt
    10321 gctgctgctc gacgaaccat gttcggcgct cgaccccatc tccaccgggc gtattgaaga
    10381 gctgatcacc gaactgaagc aggattacac cgtggtgatc gtcacccaca acatgcagca
    10441 ggctgcgcgt tgttccgacc acacggcgtt tatgtacctg ggcgaattga ttgagttcag
    10501 caacacggac gatctgttca ccaagccagc gaagaaacaa acagaagact acatcaccgg
    10561 tcgttacggt tgattcagga gtgcgttatg gacagtctca atcttaataa acatatttcc
    10621 ggccagttca acgccgaact ggaaagtatc cgcacgcagg tgatgaccat gggcggcatg
    10681 gtggagcagc agctttctga tgcaatcacc gcgatgcata accaggacag cgatctggcg
    10741 aagcgcgtga tcgaaggcga caagaacgtc aacatgatgg aagtggcgat cgatgaagcc
    10801 tgcgtgcgca ttattgccaa acgccagccg acggcgagcg acctgcgatt ggttatggtg
    10861 atcagtaaaa ccattgccga actggagcgt attggcgacg tggcggacaa aatctgccgt
    10921 actgcgctgg agaaattctc ccagcagcat cagccgttgc tggtaagttt ggagtcgctg
    10981 ggccgtcata ctatccagat gctgcacgac gtgctggacg cgttcgcgcg gatggacatt
    11041 gacgaagcgg tacgtattta tcgtgaagat aaaaaagtcg atcaggagta cgaaggtatt
    11101 gttcgtcaac tgatgaccta catgatggaa gattcgcgca ccattccgag tgtacttacc
    11161 gcgctgttct gtgcgcgttc tatcgaacgt attggcgacc gctgccagaa tatttgtgag
    11221 tttatcttct actacgtgaa gggtcaggat ttccgtcatg taggcggtga tgagctggat
    11281 aaactgctgg cgggaaaaga ccccaaagag tagtgctaac tgtaggccag ataaagcttt
    11341 ttgccgcatc tggcaatcag cccggtcata catagttacc gggctgatac actttctcac
    11401 tgaaatattg ccacacaaac tcacctcgaa actctcccc
//
LOCUS       DAZFDC010000138        11413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFDC010000138 DAZFDC010000000
VERSION     DAZFDC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.138"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            240..539
                     /gene="yoaC"
                     /locus_tag="ACHJ48_003963"
     CDS             240..539
                     /gene="yoaC"
                     /locus_tag="ACHJ48_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO4709417.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(659..838)
                     /gene="yoaH"
                     /locus_tag="ACHJ48_003964"
     CDS             complement(659..838)
                     /gene="yoaH"
                     /locus_tag="ACHJ48_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO4709418.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            912..2273
                     /gene="pabB"
                     /locus_tag="ACHJ48_003965"
     CDS             912..2273
                     /gene="pabB"
                     /locus_tag="ACHJ48_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO4709419.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRRVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPAPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2277..2855
                     /gene="nudL"
                     /locus_tag="ACHJ48_003966"
     CDS             2277..2855
                     /gene="nudL"
                     /locus_tag="ACHJ48_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO4709420.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3039..4403
                     /gene="sdaA"
                     /locus_tag="ACHJ48_003967"
     CDS             3039..4403
                     /gene="sdaA"
                     /locus_tag="ACHJ48_003967"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO4709421.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     TANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4534..6132
                     /gene="pdeD"
                     /locus_tag="ACHJ48_003968"
     CDS             4534..6132
                     /gene="pdeD"
                     /locus_tag="ACHJ48_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4709422.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFSFTINVNGPGAPALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6136..7692)
                     /gene="yoaE"
                     /locus_tag="ACHJ48_003969"
     CDS             complement(6136..7692)
                     /gene="yoaE"
                     /locus_tag="ACHJ48_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO4709423.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7680..7838)
                     /gene="yoaL"
                     /locus_tag="ACHJ48_003970"
     CDS             complement(7680..7838)
                     /gene="yoaL"
                     /locus_tag="ACHJ48_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO4709424.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8155..9126
                     /gene="manX"
                     /locus_tag="ACHJ48_003971"
     CDS             8155..9126
                     /gene="manX"
                     /locus_tag="ACHJ48_003971"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO4709425.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9189..9989
                     /gene="manY"
                     /locus_tag="ACHJ48_003972"
     CDS             9189..9989
                     /gene="manY"
                     /locus_tag="ACHJ48_003972"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO4709426.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10002..10853
                     /gene="manZ"
                     /locus_tag="ACHJ48_003973"
     CDS             10002..10853
                     /gene="manZ"
                     /locus_tag="ACHJ48_003973"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO4709427.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10908..11366
                     /gene="yobD"
                     /locus_tag="ACHJ48_003974"
     CDS             10908..11366
                     /gene="yobD"
                     /locus_tag="ACHJ48_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO4709428.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
BASE COUNT         2804 a         2875 c         2864 g         2870 t
ORIGIN      
        1 cgccctttga ttagcaactc tggttttctt tcttagctga acaatctggt tatagcgtgc
       61 ggactgaccg ttaattttct ccatcttact ggataacaca ccatgagctg ctacctttaa
      121 aacacctgaa tatcaacagg ttaactcgtg ccatatcgtt ataatccctt tgatattaga
      181 tgcaaattaa ggtcatatat agccttatcg ataacatggt taattttaag gataagaata
      241 tgcctgctgt aatagataaa gccctggatt tcattggtgc catggatgta tcagcgccaa
      301 caccaagttc gatgaatgaa agcacggcga agggaatctt taaatattta aaagaactgg
      361 gagtacccgc gagtgccgct gatattacgg cgcgagctga ccaggaaggc tggaaccctg
      421 ggttcacgga aaaaatggtt ggatgggcaa aaaaaatgga gacaggtgaa cgctctgtga
      481 ttaaaaatcc tgagtacttt tcaacatata tgcaggaaga actaaaagca ctggtctgag
      541 ttaaatttat atcagcataa atgggtcagg acgcttttaa tcacatataa aaagcgtcct
      601 ttttctcaca atcaaataat catcagcttc tataacgaaa tcgatacgga ttagtgtgtt
      661 actcgtcttc atcctcaaaa cgcgccacga ttcgctcgcc ggagtggttg gcacgcagct
      721 cttctgccac cagcgcgatt gcctggccgc tgctcatccc ttgtgccatc agttcctgga
      781 tccgctcgac agctttttgc tgctgttcat gggtgagtga aggtaaacct gcaaacattg
      841 ttaactcctg ctaaattgtt ggcgctaatt atttcatgct acccggcaca tagccagtag
      901 agtcaggact gatgaagacg ttatctcccg ctgtgattac tttaccctgg cgtcaggacg
      961 ccgctgaatt ttatttctcc cgcttaagcc acctgccgtg ggcgatgctt ttacactccg
     1021 gctatgccga tcatccgtat agccgctttg atattgtggt cgccgatccg atttgcactt
     1081 taaccacttt aggtaaagaa accgttgtta gtgaaagcga aaaacgcaca acgaccactg
     1141 atgacccgct acaggtgctc cagcaggtgc tggatcgcgc agacattcgc ccaacgcata
     1201 acgaagattt gccatttcag ggcggcgcac tggggttgtt tggctacgat ctgggccgcc
     1261 gttttgagtc actgccagaa attgcggaac aagatatcgt tctgccggat atggcagtgg
     1321 gtatctacga ttgggcgctc attgtcgacc accagcgtca tacagtttct ttgctgagtc
     1381 ataatgatgt caatgcccgt cgggcctggc tggaaagcca gcaattctca ccgcaggaag
     1441 atttcacgct cacttccgac tggcaatcca atatgacccg cgagcagtac ggcgaaaaat
     1501 ttcgccgggt acaggaatat ctgcacagcg gtgattgcta tcaggtgaat ctcgcccagc
     1561 gttttcatgc gacctattct ggcgatgaat ggcaggcatt ccttcagctt aatcaggcca
     1621 accgcgcgcc atttagcgct tttttacgtc ttgaacaggg tgcaatttta agcctttcgc
     1681 cagagcggtt tattctttgt gataatagtg aaatccagac ccgcccgatt aaaggcacgc
     1741 taccacgcct gcccgctcct caggaagata gcaaacaagc agaaaaactg gcgaactcag
     1801 cgaaagatcg tgccgaaaat ctgatgattg tcgatttaat gcgtaatgat atcggtcgtg
     1861 ttgccgtagc aggttcggta aaagtaccag agctgttcgt ggtggaaccc ttccctgccg
     1921 tgcatcatct ggtcagcacc ataacggcgc aactaccaga acagttacac gccagcgatc
     1981 tgctgcgcgc agcttttcct ggtggctcaa taaccggggc tccgaaagta cgggctatgg
     2041 aaattatcga cgaactggaa ccgcagcgac gtaatgcctg gtgcggcagc attggctatt
     2101 tgagcttttg cggcaacatg gataccagca ttactatccg cacgctgact gccattaacg
     2161 gacaaatata ctgctctgcg ggaggtggaa ttgtcgccga tagccaggaa gaagcggaat
     2221 atcaggaaac ttttgataaa gttaataaga tattacgcca actggagaag taagacgtgg
     2281 aataccgtag cctgacgctt gatgattttt tatcgcgctt tcaacttttg cgcccgcaaa
     2341 ttaaccggga aaccctaaat catcgtcagg ctgccgtgtt aatccccatc gtccgtcgac
     2401 cgcaaccggg gttgttgctg actcagcgtt caattcatct gcgtaaacac gctggacaag
     2461 tggcattccc tggaggtgca gtcgatgaca cggacgcatc agttatcgcc gccgcgctgc
     2521 gcgaagctga agaagaggtt gctataccgc cttccgccgt tgaagttatc ggcgtgctgc
     2581 cgcccgtcga tagcgtaact ggctaccagg taaccccagt ggtcggcatt atcccgcccg
     2641 atctgccgta tcgcgccagt gaagatgaag tttccgctgt gtttgaaatg ccgctcgccc
     2701 aggcattaca tctgggtcgt tatcaccctt tagatatcta ccgccgcggc gattcacatc
     2761 gggtatggct atcctggtac gaacagtatt ttgtatgggg aatgaccgca ggcataattc
     2821 gtgagctggc gctgcaaatt ggtgtgaaac cctgactata cttatcttta catctacaaa
     2881 acactacttg agacaatcat cgcaatatta gttaaatcgc ggtttttgat tagtttaatt
     2941 catgtgaata gttaagccag tcgccgcgtt ccctcttaca ctatgcgctg ttattagttc
     3001 gttactggaa gtccagtcac cttgtcagga gtattatcgt gattagtcta ttcgacatgt
     3061 ttaaggtggg gattggtccc tcatcttccc ataccgtagg gcctatgaag gcgggtaaac
     3121 agttcgtcga tgatctggtc gaaaaaggct tactggatag cgttactcgc gttgccgtgg
     3181 acgtttatgg ttcactgtcg ctgacgggta aaggccacca caccgatatc gccattatta
     3241 tgggtcttgc aggtaacgaa cctgccaccg tggatatcga cagtattccc ggttttattc
     3301 gcgacgtaga agagcgcgaa cgtctgctgc tggcacaggg acggcatgaa gtggatttcc
     3361 cgcgcgacaa cgggatgcgt tttcataacg gcaacctgcc gctgcatgaa aacggtatgc
     3421 aaatccacgc ctataacggc gatgaagtcg tctacagcaa aacttattat tccatcggcg
     3481 gcggttttat cgtcgatgaa gaacactttg gtcaggatac tgccaacgaa gtgagcgtgc
     3541 cgtatccgtt caaatctgcc accgaactgc tcgcgtactg taatgaaacc ggctattcgc
     3601 tgtctggtct cgctatgcag aacgaactgg cgctgcacag caagaaagag atcgacgagt
     3661 atttcgcgca tgtctggcaa accatgcagg catgtatcga tcgcgggatg aacaccgaag
     3721 gtgtactgcc aggcccgctg cgcgtgccac gtcgtgcgtc tgccctgcgc cggatgctgg
     3781 tttccagcga taaactgtct aacgatccga tgaatgtcat tgactgggta aacatgtttg
     3841 cgctggcagt taacgaagaa aacgccgccg gtggtcgtgt ggtaactgcg ccaaccaacg
     3901 gtgcctgcgg tatcgttccg gcagtgctgg cttactatga ccactttatt gaatcggtca
     3961 gcccggacat ctatacccgt tactttatgg cagcgggcgc gattggtgca ttgtataaaa
     4021 tgaacgcctc tatttccggt gcggaagttg gttgccaggg cgaagtgggt gttgcctgtt
     4081 caatggctgc tgcgggtctt gcagaactgc tgggcggtag cccggaacag gtttgcgtgg
     4141 cggcggaaat tggcatggaa cacaaccttg gtttaacctg cgacccggtt gcagggcagg
     4201 ttcaggtgcc gtgcattgag cgtaatgcca ttgcctctgt gaaggcgatt aacgccgcac
     4261 ggatggctct gcgccgcacc agtgcaccgc gcgtctcgct ggataaggtc atcgaaacga
     4321 tgtacgaaac cggtaaggac atgaacgcca aataccgcga aacctcacgc ggtggtctgg
     4381 caatcaaagt ccagtgtgac taatacttct tactcgccca tctgcaacgg atgggcgaat
     4441 ttatccccgc tttctcgtct gctgtaatat tccccactac acttccactg ttgcgtcagg
     4501 cgtttgtcgc catacgctta cagagtggcc cgcatgcaaa aagcacaacg gatcattaaa
     4561 acctatcgcc gtaatcgaat gattgtttgt acgatttgcg cactcgttac gctcgcttcg
     4621 accctgagcg tgcgatttat ttcacagcgt aacttaaatc aacaacgggt agtacaattc
     4681 gccaatcacg ctgtagagga attagataaa gtactgcttc ccctacaggc aggtagcgaa
     4741 gtcttgcttc cgctgattgg tctgccctgc tctgtcgccc atttgccatt acgtaaacag
     4801 gcggcaaaac tccaaactgt gcgatccatt ggcctggtgc aagacggcac actttattgc
     4861 tccagcattt ttggttatcg caatgtgccc gtcgtggaca ttctggctga acttcctgca
     4921 ccgcaaccac ttttacgcct gacgaccgac cgtgtcctga ttaaaggcag tccggttttg
     4981 attcaatgga cgcctgcagc gagcagtagc aatgctgggg tcatggagat gattaacatc
     5041 gacttactga cggcaatgct gcttgagcca caactgccgc aaatcagtag cgccagcctg
     5101 acggtggaca aacggcattt gctctatggt aatgggctgg tagattccct tccacaacct
     5161 gaagacaatg aaaactatca ggtttcttcg caacgctttt cttttaccat taacgttaat
     5221 ggtccggggg ctccggcgct ggcatggcac tatcttccaa cacaattacc gctggcggta
     5281 ctgctaagtt tactggtggg ctacatcgcc tggctggcga ccgcttaccg aatgagcttt
     5341 tcccgcgaaa tcaatctggg cctggcgcaa catgagttcg aattgttctg tcagcctttg
     5401 cttaatgcgc gcagccagca atgtattggt gtagagattt tgctgcgctg gaacaatccg
     5461 cgtcagggct ggatttcacc ggatgtgttt attcctatcg cggaagaaca tcatttaatt
     5521 gtgccactga cccgctatgt gatggcagaa accattcgtc agcgccatgt tttcccgatg
     5581 agtagtcagt ttcatgttgg cattaacgtc gcacccagcc attttcgccg tggtgtgctg
     5641 ataaaagatc tcaatcagta ctggtttagc gctcacccga ttcagcaact gatcctcgaa
     5701 atcaccgaac gcgatgcctt actggatgtt gattatcgga ttgcccgcga gctacatcgt
     5761 aaaaacgtca aactggcgat tgatgacttc ggcaccggca acagttcatt ttcctggctt
     5821 gaaacattac gtcctgacgt gctgaaaatt gataagtcat ttaccgcagc tataggttct
     5881 gacgcggtta actcgacggt gaccgatatc atcatcgcgc tggggcaaag actgaatatt
     5941 gaactggtgg cggagggcgt ggaaacgcaa gaacaggcga agtatttgcg ccgtcatggc
     6001 gtgcatattt tgcaagggta tttgtacgca cagccgatgc cgctacgtga ttttcccaaa
     6061 tggctggcgg gcagccaacc gccgcccgcc cggcataatg gacatatcac gcccattatg
     6121 ccgttacgtt aacgcttact catcttcatc gtgcgccggt tgctctttaa caatgcgaac
     6181 cagatcaaca cgataatcat tggcttcaat gatggtgata tgcagtggcc ctacatcaat
     6241 cacatcgccc acacggggaa tgtgaccatt tgccgagatc acgaggcccg cgaccgtcgc
     6301 gatatcatcg tcatcggcaa ggtgctcaac atcaagcgcc tgctgcaagg catgcaaatc
     6361 tgtaccgcct tttaccagcc agccgtcacc atcagtaatg atttccggcg tttcgtcagc
     6421 gtccgggaat tcacccgcaa tggcttccag cacatccagc ggcgtgacca agccttgtac
     6481 cacaccaaac tcgttggtca cgataacaaa gctcccgcga gcacgacgca gcacgcccag
     6541 caggttgatc ggatcgaggg tttcagggac gataatcgcc ggagacgccg aagcaatcgc
     6601 cgccacatca acgccctctt ccagcgccac cagcagttct ttagcacgta cgattccgat
     6661 gatttcatcc agttcaccgc gacataccgg gaacaggctg tgcggtgagg agagcagctg
     6721 ctcgcggatt tcatcgaccc cgagattagc gtcaacccag cttatttcac cgcgcggcgt
     6781 catgatcccg cgcagagaac gcgacgccag cgtcagtacg ccgttaatca tgtaacgttc
     6841 ttcttcggca aatgcacctt ccgggatcgg catcggcatc gggttatcgg catcgtgctg
     6901 aacattggcc tgacgtttcc cgcccatcaa acgcaggatg gcatcggcag tacgcgctcg
     6961 cagcggcaaa gtcgactggt gacgaataaa gttgcgacgc gcaatctggt taaacacttc
     7021 gatgatgatc gagaagccaa tcgcggcata caggtaacct ttcggaatgt ggaaaccgaa
     7081 accttctgcc accagactca gaccaatcat taacaggaag ctcagacaga gcaccaccac
     7141 cgtggggtgc tggttaacga atcgcgtcag cggtttggat gccagcaaca taaccgccat
     7201 cgcaatcact accgccgcca tcatcaccgg cagatggtta accatcccta ctgcagtaat
     7261 taccgcatcc aacgagaaga cggcgtcaag gatgacaatc tgtgtgacga ccacccagaa
     7321 actggcgtag cctttaccgt ggccggaatc atgatcgcgg ttttccagcc gttcatgcag
     7381 ttcggttgtt gctttgaaca gcaagaatat ccccccgaac aacataatca ggtcgcgtcc
     7441 ggagaaggag aaatccatga cggtaaatag cggtttggtc agcgtgacca tccatgaaat
     7501 cagcgacagc agccccagac gcataatcag cgccagtgat aaccccagca aacgcgcttt
     7561 atcgcgttgt tttggcggca gtttgtcagc aagaatggcg atgaagacca ggttatcgat
     7621 acccagcaca atttcgagaa caacaagcgt gagtagcccc gcccaaattg aggggtccat
     7681 taagaattcc atgacaagct cctgcttaag gaatagctat tcgacgccag aaataatgca
     7741 ggcgtaacga caaaatgcaa acgaaaggtg cggcatagag tgccagaaag gcaggcgtta
     7801 aaaggcctga tgctgaaatg acgtcggtga cgatccatac tgcgggctac tgccctatac
     7861 tccatgattg ttaaacggga gttaaacata tcagagacgc ctctgatttg gcaaagattt
     7921 accttccttt gcaaacgaat gtgacaagga tattttacct ttcgaaattt ctgctaatcg
     7981 aaagttaaat tacggatctt catcacataa aataattttt tcgatatcta aaataaatcg
     8041 cgaaacgcag gggtttttgg ttgtagccct tatctgaatc gattcgattg tggacgacga
     8101 ttcaaaaata catctggcac gttgaggtgt taacgataat aaaggaggta gcaagtgacc
     8161 attgctattg ttataggcac acatggttgg gctgcagagc agttgcttaa aacggcagaa
     8221 atgctgttag gcgagcagga aaacgtcggc tggatcgatt tcgttccagg tgaaaatgcc
     8281 gaaacgctga ttgaaaagta caacgctcag ttggcaaaac tcgacaccac taaaggcgtg
     8341 ctgtttctcg ttgatacatg gggaggcagc ccgttcaatg ctgccagccg cattgtcgtc
     8401 gacaaagagc attatgaagt cattgcaggc gttaacattc caatgctcgt ggaaacgtta
     8461 atggctcgtg atgatgaccc aagctttgat gaactggttg cgctggcagt agaaacaggc
     8521 cgtgaaggcg tgaaagcact gaaagccaaa ccggttgaaa aagccgcgcc agcacccgct
     8581 gccgcagcac ccaaagcggc tccaactccg gcaaaaccaa tggggccaaa cgactacatg
     8641 gttattggcc ttgcgcgtat cgacgaccgt ctgattcacg gtcaggtcgc cacccgctgg
     8701 accaaagaaa ccaatgtctc ccgtattatt gttgttagtg atgaagtggc tgcggatact
     8761 gttcgtaaga cactgctcac ccaggttgca cctccgggcg taacagcaca cgtagttgat
     8821 gttgccaaaa tgattcgcgt ctacaacaac ccgaaatatg ctggcgaacg tgtaatgctg
     8881 ttatttacca acccaacaga tgtagagcgt ctcgttgaag gcggcgtgaa aatcacctct
     8941 gttaacgtcg gtggtatggc attccgtcag ggtaaaaccc aagtgaataa cgcggtttcg
     9001 gttgatgaaa aagatatcga ggcgttcaaa aaactgaatg cacgcggtat tgagctggaa
     9061 gtccgtaagg tttccaccga tccgaaactg aaaatgatgg atctgatcag caaaatcgat
     9121 aagtaacgta ttgtgttgat tatcagtcag ttttcacact ttagtcatac gtaaacagga
     9181 gaagtacaat ggagattacc actcttcaaa ttgtgctggt atttatcgta gcctgtatcg
     9241 caggtatggg atcaatcctc gatgaatttc agtttcaccg tccgctaatc gcgtgtaccc
     9301 tggtgggtat cgttcttggg gatatgaaaa ccggtattat tatcggtggt acgctggaaa
     9361 tgatcgcgct gggctggatg aacatcggtg ctgcagttgc gcctgacgcc gctctggcat
     9421 ctatcatttc taccattctg gttatcgcag gtcatcagag cattggtgca ggtattgcac
     9481 tggcaatccc tctggccgct gcgggccagg tactgaccat catcgttcgt actattaccg
     9541 ttgctttcca gcacgctgcg gataaggctg ctgataacgg caacctgaca gcgatttcct
     9601 ggatccacgt ttcttctctg ttcctgcaag caatgcgtgt ggctattccg gccgtcatcg
     9661 ttgcgctgtc tgtaggtacc agcgaagtac agaacatgct gaatgcgatt ccggaagtgg
     9721 tgaccaatgg tctgaatatc gccggtggca tgatcgtggt ggttggttat gcgatggtta
     9781 tcaacatgat gcgtgctggc tacctgatgc cgttcttcta cctcggcttc gtaaccgcag
     9841 cattcaccaa ctttaacctg gttgctctgg gtgtgattgg tactgttatg gcagtgctct
     9901 acatccaact tagcccgaaa tacaaccgcg tagccggtgc gcctgctcag gcagctggta
     9961 acaacgatct cgataacgaa ctggactaac aggtgagcga aatggttgat acaactcaaa
    10021 ctaccaccga gaaaaaactc actcaaagtg atattcgtgg cgtcttcctg cgttctaacc
    10081 tcttccaggg ttcatggaac ttcgaacgta tgcaggcact gggtttctgc ttctctatgg
    10141 taccggcaat tcgtcgcctc taccctgaga acaacgaagc tcgtaaacaa gctattcgcc
    10201 gtcacctgga gttctttaac acccagccgt tcgtggctgc gccgattctc ggcgtaaccc
    10261 tggcgctgga agaacagcgt gctaatggcg cagagatcga cgacggtgct atcaacggta
    10321 tcaaagtcgg tttgatgggg ccgctggctg gtgtaggcga cccgatcttc tggggaaccg
    10381 tacgtccggt atttgcagca ctgggtgccg gtatcgcgat gagcggcagc ctgttaggtc
    10441 cgctgctgtt cttcatcctg tttaacctgg tgcgtctggc aacccgttac tacggcgtag
    10501 cgtatggtta ctccaaaggt atcgatatcg ttaaagatat gggtggtggc ttcctgcaaa
    10561 aactgacgga aggggcgtct atcctcggcc tgtttgtcat gggggcattg gttaacaagt
    10621 ggacacatgt caacatcccg ctggttgtct ctcgcattac tgaccagacg ggcaaagaac
    10681 acgttactac tgtccagact attctggacc agttaatgcc aggcctggta ccactgctgc
    10741 tgacctttgc ttgtatgtgg ctactgcgca aaaaagttaa cccgctgtgg atcatcgttg
    10801 gcttcttcgt catcggtatc gctggttacg cttgcggcct gctgggactg taagactgtt
    10861 gtacactacc ggggcctttt ggccccgttt ttttatctgg aggattaatg acaatcacgg
    10921 acctggtact gattcttttc atcgccgcac tcctggcctt cgcgatctac gatcagttca
    10981 tcatgccccg ccgtaacggc cccaccctgc tggcaattcc tttgctccgg cgtggtcgca
    11041 tcgatagcgt tatcttcgtc ggattgattg tcattcttat ctataacaac gtcacgaatc
    11101 atggtgcgtt aataacgaca tggttattaa gcgcactggc tctgatgggt ttttatatat
    11161 tctggatccg cgttccgaag atcatcttta aacaaaaagg ttttttcttc gccaatgtct
    11221 ggattgaata tagccgaatc aaagcgatga acttgtcgga agatggcgtg ttggtgatgc
    11281 aattagaaca gcgtcggctg ttaatccgcg ttcgaaatat cgacgatctg gaaagaattt
    11341 ataaacttct cgtttcaact caatgagtta ttaatttagc caaagctatg tttagtgtat
    11401 ttttaataat cag
//
LOCUS       DAZFDC010000139        11343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFDC010000139 DAZFDC010000000
VERSION     DAZFDC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.139"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(262..1746)
                     /locus_tag="ACHJ48_003975"
     CDS             complement(262..1746)
                     /locus_tag="ACHJ48_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4709429.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKFRKEALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(1933..2886)
                     /gene="ompT"
                     /locus_tag="ACHJ48_003976"
     CDS             complement(1933..2886)
                     /gene="ompT"
                     /locus_tag="ACHJ48_003976"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO4709430.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVSVNAGYYVTPNAKVYVEGTWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            3385..3969
                     /locus_tag="ACHJ48_003977"
     CDS             3385..3969
                     /locus_tag="ACHJ48_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4709431.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(3994..4086)
                     /locus_tag="ACHJ48_003978"
                     /pseudo
     CDS             complement(3994..4086)
                     /locus_tag="ACHJ48_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            4087..4833
                     /locus_tag="ACHJ48_003979"
                     /pseudo
     CDS             4087..4833
                     /locus_tag="ACHJ48_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(5205..5471)
                     /gene="minE"
                     /locus_tag="ACHJ48_003980"
     CDS             complement(5205..5471)
                     /gene="minE"
                     /locus_tag="ACHJ48_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO4709432.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(5475..6287)
                     /gene="minD"
                     /locus_tag="ACHJ48_003981"
     CDS             complement(5475..6287)
                     /gene="minD"
                     /locus_tag="ACHJ48_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO4709433.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(6311..7006)
                     /gene="minC"
                     /locus_tag="ACHJ48_003982"
     CDS             complement(6311..7006)
                     /gene="minC"
                     /locus_tag="ACHJ48_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO4709434.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            7526..7894
                     /gene="ycgJ"
                     /locus_tag="ACHJ48_003983"
     CDS             7526..7894
                     /gene="ycgJ"
                     /locus_tag="ACHJ48_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO4709435.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLSEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSRKINQTTTKMLFMCRE"
     gene            complement(7997..8398)
                     /gene="pliG"
                     /locus_tag="ACHJ48_003984"
     CDS             complement(7997..8398)
                     /gene="pliG"
                     /locus_tag="ACHJ48_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO4709436.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            8469..8627
                     /locus_tag="ACHJ48_003985"
     CDS             8469..8627
                     /locus_tag="ACHJ48_003985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709437.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            8639..8932
                     /locus_tag="ACHJ48_003986"
     CDS             8639..8932
                     /locus_tag="ACHJ48_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO4709438.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            9004..9663
                     /gene="ycgM"
                     /locus_tag="ACHJ48_003987"
     CDS             9004..9663
                     /gene="ycgM"
                     /locus_tag="ACHJ48_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO4709439.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            9755..10201
                     /gene="ycgN"
                     /locus_tag="ACHJ48_003988"
     CDS             9755..10201
                     /gene="ycgN"
                     /locus_tag="ACHJ48_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO4709440.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(10408..11319)
                     /gene="hlyE"
                     /locus_tag="ACHJ48_003989"
     CDS             complement(10408..11319)
                     /gene="hlyE"
                     /locus_tag="ACHJ48_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO4709441.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGNIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AANKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         2989 a         2395 c         2601 g         3358 t
ORIGIN      
        1 gctctgggta tgaagctgtt tgtaactcat gttacagtaa tggctattac tatctcgaag
       61 gtgcaaaatg tatattgatt tgatatttta taaatttact tcgaatctgg cacgtcaact
      121 tttgattaaa gtaatatcag ttttcggcga aaaaaggacc gtgtacatga gacgatttca
      181 tacaagaata acaaaaatat gaatatgctc ccaatgcaat atatgttttg cattgggagc
      241 aaatagcgga atattttgaa attattgtat ttctgcttca gaatttcctg ggcagtatat
      301 attttctatt ttttgtggag tgatcaggtg atgtacaact gctttatcta cacataaatt
      361 aactccggtg agagctaatg tatgcccatc accatttatt gttagtaacg ggctggaaaa
      421 tttctctgcc atcttacggg cattaatcca gggcgttgtt gggtcgtatt tgtgtgctac
      481 aaacagtaaa ccagagggca gaacagtatt tttcaggcga gttttgttca ggtcgctatg
      541 tattggccat aattcacaaa aatcaggtga atcggaacgt ccattgtcaa agttaatagc
      601 cgggaaggca ttcgcaagag cctcttttcg gaattttcgc tcttctggtg ttaattgctc
      661 atccccctga tctacacaaa ggattacccc cgaagcatca cttgactctt ctgaggctat
      721 cggagcactg agcgcagttt caatttcatt actgacaatc ccctgagaga actggcgtat
      781 ggcagttgca agggttggcc atgatgaacg ccatagcaga aggtctgttg ttaatgatat
      841 gagttcatct gaagatatat tttctccctt actgtctaat aaaggtttgt gatgtaattt
      901 tgataatagc tcatggaact gagttattgc cttatctctg tctgaagaaa gcgggcaact
      961 ttttgtacgc gcacaccagg atgcaaagcg atcaaacgtt tcctgataac tctgtgcctg
     1021 tttgagttgc catgtgaagt tgtcctccag gtcatcgata tcgacgactc catcaagaac
     1081 gatagatctt acgttgtagg gaaaacgttc tgcatataag gctgcaattt gagttccata
     1141 cgaatacgcc acggctgtca gttgtttatc ccccaaggct tgcctaatac gatcaatatc
     1201 gtatacagcc tcgttagagc cgatatggcg aatgacttcg gctccggtat tatggataca
     1261 ggcattaatt ttatttaata cttgttgctt ttcggttatg ttttcctgag tctctgtatc
     1321 tgattgccgg cagtttattg tcggagtgga ctgcccgacg cctcgaggat caaatccaat
     1381 aatatcccat gactcacgaa gatttgtgac tggccagtca aagttaatat aaggatttat
     1441 gcctggtaac ccgggaccac cacttattat caggatactt cctttatgct tgctttttgc
     1501 cggcaatttt gtcaacgcta gtttgacttg tgattttttt tcataagaag catctccgcc
     1561 tgtgtctgta tattttaatg gaacagacaa ataaccacat agtaagtcag gagacggttt
     1621 ttcctcacca aaccagtggt tgaattgact ggccatacag gattgccact gtatctgctg
     1681 ggcagatacg gttactggta gaagtaacgt taaaacaact ttgaaatgag taattatttt
     1741 tcgcattgtg tctctgaata tcggaataaa gataaaattt gaatatattg aggtcttgtg
     1801 ttgcggtaag agattacacg ttatgacata ggttaaatgc ttacaaaatt agtggatatt
     1861 gtctacttgt aactgtaaac aaattccccg gggttataca ataccaccgg ggagaaaatc
     1921 tggttaactt cgttaaaagg tgtacttaag accagcagta gtgatgaagt tatagttttc
     1981 tatgcctgca ccatttttgc tgtagtctga agtgttatca ttgtgatcat aaagtgaagt
     2041 attacctttt ttattcgtaa cccgattcca tgtgccttca acataaactt ttgcgttagg
     2101 tgttacgtaa taacctgcat tgactgaaac agaatagtaa ttttggtctt tgactttact
     2161 gcgataagtg attctttttc ctgggtcata gtgctcatcg ttatcagatg cttccaccca
     2221 gccgctgtat ttaaatgtgc cacctagctc aaaatcttca taacgataac ttccagtcaa
     2281 gccaatgtag ggcattttaa aacgttgttt gtagccgatt gctctttctc cattcgggaa
     2341 ggagccgata tcatctctga atccctcctc agaactgtag atataggaac cacctctggc
     2401 tgtaaagcta taacggcttt cctgatatcc ggccatgagt cccaggcggt aattgggttc
     2461 gttgaggagc cagcctttga tattcagatc aaattcgttg gcataattga gttgtgtatc
     2521 agggtgtcta ctttcatccg tccaggttcc ggggttactg gaatccatcc agtcctgatc
     2581 gaccatattg ccacctcggc tgccgagagt tgtccagcca gcagccccga tagatatctg
     2641 gggcatcaaa tcccaattaa ttgcaccttt aataattgca gcgttattga atttccagtc
     2701 gagttgactg acttttcggc ctccttcttc ggctagataa acacgctctt ttgtttttcc
     2761 gctcagagtt ccaagactaa tgtccgcatt tatgttgtca ggagtaaacg ataaagtctc
     2821 ggtagaagca aaagagctga tcgcaatagg ggttgtcagg actattccca gaagtttcgc
     2881 ccgcataaaa ggtctccatt caatcgtttt aatgattgaa tatgtatttt ttatatctaa
     2941 cttaatgagt caattgtata ttgtttaact gtttatggtt tgtttaagtt tgaattattg
     3001 tttcgtttta ttcattcttt atgggcagct tgccaagtgt tgtattattg ttgtggttta
     3061 atatgctgga aattttcttt ttgctgatat ttttatctgt ctatagttat tcatttatac
     3121 gcatattagc aaaaaaaata ttattttagg tatttctaca gtacatttga cacgatgatt
     3181 aacctctatg tagctaggtt tttgtaaaga aactgtgcgt cactgtatcc aggactgtta
     3241 tgtgccaaat aaggaagttg aagttaccag ctcttactct aaagctataa cgtatagaaa
     3301 aaatgcattt gagacgtgta aaatcattta tttaatcgca atttatgtaa tggcataatc
     3361 aggtcggtga aagaggggag aagaatggat aaaagagcta aaaatcagat agtcgatagc
     3421 gatattgcac gtttgttact caagctcaga aagtctcgaa acttgactgt tacagagctt
     3481 gcccaacgat ctggtgtcag tcaggcgatg atcagcaaag ttgaacgagg cacttcaagc
     3541 cccagtgcaa caatacttag tcggttagct aatgcactga atattacgct atcaaagctt
     3601 tttgcagagc ttgagatgca acagaattca cttgtacttt tggctgacca gcaacaacat
     3661 tggattgatg aagaaacagg cattacccgc tggtctttgt ccccagcggg tgcatgtccg
     3721 gaactaataa aagttgaaat cccccctatg gggcaattga ctatccctgc ctctgcaaat
     3781 gagcatcttt ctggacaaac tctatggatg ttaagcggca gccttgattt tcgagtcaat
     3841 catcagactc atcatttaca agccggtgat tgtcttgctc ttacctttgc cagcgagtac
     3901 caaatgagaa acccagatag cggccagagt tgttcttaca ttgtcgcttt cagccaaaaa
     3961 aatccatgat gtaattctcc taaaattatc atgtcacatt gcgttataaa gatgtaatag
     4021 agggtagggt ctgccttgat tgtcccgctc ggaacgtcct gaaagtttaa agcccatatg
     4081 ttgataaatg cgggtgccgg gtttgagcag acgctgaccg gaatgacagt ggggatcgac
     4141 agccgtaatg atattcctga ggggattgcg acgctgggcg cttttatggg ttattcccat
     4201 tcacatatcg gttttgatcg tggaggacat ggcagtgtgg acagttattc tctgggcggc
     4261 tatgccagtt gggaacatga aagtggtttc tatctggacg gtgtcgtgaa gctgaaccgt
     4321 tttgaaagta acgtagccgg taaaatgagc agcggtggag ccgccaatgg cagttaccat
     4381 agcaacgggc tgggcggtca cattgaaacc gggatgcgat ttaccgatgg taactggaac
     4441 ctgacgccgt atgcctcgtt aacggggttc accgctgata accccgaata tcatttatcc
     4501 aatggcatgg aatcgaaatc agtcgatacc cgcagtatat atcgtgaact gggtgcaacg
     4561 ctgagttaca acatgcgtct ggggaacggt atggaagttg agccgtggct gaaggcggct
     4621 gtgcgcaaag aatttgtcga tgataaccgg gtgaaagtga atagtgacgg taatttcgtc
     4681 aatgatttgt cgggcagacg tggaatatac caggcaggta ttaaagcctc attcagcagt
     4741 acgttaagcg ggcatcttgg ggtggggtat agcaacggtg ctggtatgga atccccgtgg
     4801 aacgcggtgg ctggtgtgaa ctggtcgttc tgaccatcaa cgattaaact gcgcttcggc
     4861 gcagttttcg tttacaggat gttaaaagag aaaattctgg ggtaaaaaaa gcccgccagt
     4921 tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg gggttatcat
     4981 ctcccagtat atcaacacta acaataaggt tatttactca accaggcata aatattttgt
     5041 tttgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca atgaggagta
     5101 tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa aactcaaggc
     5161 agagataact ctgccttgaa gataaatgcg cttttacagc gggattattt cagctcttct
     5221 gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt ttgctcaagc
     5281 tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc aagaatatct
     5341 ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc agcaacaata
     5401 atctgcagcc gttcttttgc aatgttggct gtatttttct tccgcgagag aaagaaatcg
     5461 agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt ctcttcttca
     5521 atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc gtaggcttta
     5581 cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc gcgcaatact
     5641 gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag cacatcttcc
     5701 atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa cagcaggtgc
     5761 tctttaatag gctcttcgcc attttctgcg cggcgtgatt ttgacgccag aatgcctaaa
     5821 atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat ggcttcgtct
     5881 gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc acaaacgata
     5941 aattcaaaat ccatcgcttt cagatcatca agaactttag cgaccccttc acgggtgagg
     6001 gcatctttat cgcgtgtttg cgatgccggc agaatataga gattttcagt acgcttatct
     6061 ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa atcgtaaacg
     6121 acccggcgtt cacaacccat aatcaggtcg agattacgca ggccgatatc aaaatctatc
     6181 acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct ggaggttgtc
     6241 ttaccaacac cccctttgcc cgaagtaaca acaataatgc gtgccataga aattccttgt
     6301 taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac tgcagtcgcg
     6361 ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca cctgcgatgg
     6421 acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca ccacttgccc
     6481 ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg gcaatcaatt
     6541 cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca taaatacgct
     6601 gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtt gtattttgcg
     6661 ctggagcctg cggtgcggga gctggacgtg gcgctttttc ctttccttcc gtcaggatag
     6721 gcagccccat tttttcaatt tcggctttaa gttgcgcatc tttgcagcca cttacgccaa
     6781 taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt accgggtctt
     6841 ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg gcctgagcga
     6901 ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga tgaaccacag
     6961 ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg gccttactca
     7021 attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag gcatgttata
     7081 gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag tctattctgt
     7141 gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc attgccgtcg
     7201 tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg acagcatctc
     7261 cagacctgtt taaatatcca gtattgaggc aagtgtattt tttcaccacg ccaggctttc
     7321 ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct taatatccgc
     7381 cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt caggtcatcg
     7441 taactcgtcc aggtcagcgg ttttgattac ggattgatga tgaaatgagc ataagtgttg
     7501 tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attttttata tcggattgtc
     7561 aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg gacttcaatc
     7621 tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg gcatttcccc
     7681 agagttaaca aggaaatatc tcagcgaaaa agccgctgaa aacttacaat cattacaagg
     7741 ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt
     7801 atgccgtgat gatcgttatt ttggtgtaga tggtaagcgg agtagaaaaa tcaatcaaac
     7861 caccacaaag atgttattta tgtgtcgtga gtgatgtctt gtttacatta tgttctattc
     7921 gtcattaacc tcttgagcga cagagaggag tggggcgtca acgtttatga cggggagact
     7981 ccccgtcggt tgacatttac ttaatttgaa tatcgacgtt atatttttta gttttgtttt
     8041 tacgggcgtc gtttcgggtt tgcagcactc gcagctcata tttgccagaa gcagggagtg
     8101 agtattgtcc gtgactatcg agttcagggg aatatctgga taaatcaaca gaatcatcga
     8161 ttcctggccc gaagaggtaa gtatcagcgc cttcattaga gatactcaca tgcactttct
     8221 gacctttttt ggcatagaag gtatatgtat cgtaatcgta tccctttatt tcgccggagt
     8281 actgagcgct gctatggcct tttctgaact caacattgac attcttaccc gcagcaaagc
     8341 tcgtggaggt tagcagtgca agcagcaata cagccttact gatgctcttg attttcattg
     8401 gtagtcttct gttcagaatg agtacgatga actgttataa tataacaatc ccttacggga
     8461 gaattccagt ggctttcctg cgttcggtaa aatcccgtcg cggcattttt gccgggggga
     8521 ttacttataa aggggcatgg actcaacaaa ccgtagaaat ttacagctgg ctggcttgtg
     8581 gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga gtaaaagtat
     8641 gttttgtgtg atttatcgaa gcagcaagcg tgaccagacc tatttatatg tcgaaaaaaa
     8701 agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc ctcagttagc
     8761 gatgattctg ccgctggatg ggcggaagaa actggtcaat gccgatattg aaaaggttaa
     8821 acaggcatta accgagcaag gttactattt gcagttaccg ccaccacccg aagatttgct
     8881 gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat aaccgatatc
     8941 cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg tgaggggaac
     9001 gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt gagtaaagta
     9061 gtttgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc agtgcccgaa
     9121 gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca gccattggcg
     9181 atcccatccg atttcggttc agttcatcat gaagtcgaac tagcggtgtt gattggcgcg
     9241 acgctgcgtc aggctacgga agagcatgtc cgcaaagcca ttgccggtta cggcgtggcg
     9301 ctcgatctga cgttgcgtga tgttcaggga aaaatgaaga aagccgggca gccgtgggaa
     9361 aaggctaaag cgtttgataa ctcttgtccg ctttccgggt ttattcccgc ggcggaattc
     9421 accggcgatc cgcaaaatac aacgctgggc ctgagcgtaa acggcgaaca acgccagcaa
     9481 ggtacgactg cggacatgat ccataaaatc gttccgctga tcgcttatat gagcaagttt
     9541 tttaccctca aggccggtga cgttgtgctg acaggcacgc ctgatggcgt cggcccgttg
     9601 caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac tcgcgttttg
     9661 taatactttt tgccgcctga aagcggcggc aaaacttgca tcgctgtgcc agactggtta
     9721 taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt tctggcaaag
     9781 taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg gttgcggtca
     9841 gtgttgcctg cataaactga tggatgaaga caccgacgaa atctacttca ctaacgtcgc
     9901 ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc gtttcgagtt
     9961 tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat ggctaccaat
    10021 gacctgcgct tatcgtttgc tggcggaagg taaagattta cctgcgtggc atccgctgct
    10081 tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata tcgcagtgaa
    10141 agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact gggcgcagtg
    10201 ataaatttaa tagatgttga tgggggcttt cttaatatac gtctggatgc tcgccatttc
    10261 gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc ttattatcaa
    10321 caggtaactc tcaatcttta aaaaacgtct gtatttttaa atgtgataaa ctttatacct
    10381 tgagtacatg gagagaataa ttgctgatca gacttcaggt acctcaaaga gtgtcttctt
    10441 accgtgtctt ttctgatact cattacaggt gttaatcatt ttattggccg cttcttttag
    10501 caaagaaagc attaaatcat cataatcaac atagaatctg gttgtttcag tttccgtttt
    10561 tatctccccg atggcggcta tttcggtggt taatttcaat ttggcggcat cgatatcttt
    10621 attcgcttgt ttaaccgtgt tagacagggt ggtaaagaaa ctctgcacag attttaactt
    10681 gttcttcaat tctggaatca gtttcccttc aactacgccc gcagcaatag aataggaaat
    10741 gattaaacca aatggaccgg cgacgacacc ggctgcggca ccggcatacg cttccttcct
    10801 gattttatct acctgtgact ggaaatagct gcttttttct gaaaaatcat tggttaactg
    10861 gctatctaac gccagcagtt tcccggaagc gttgttgaaa ctttgtgagc ttaccagcag
    10921 ggatttttgc gcttcattca gcttcgtgat gccgtcatcc agtaccttaa tgagaatgtc
    10981 tttctgggcg gatgctttct tctcattata ctcatcaaat agcaaaatat acgctgcgag
    11041 caattgcgtc gcaacaccac accattcata cactgtttgg gttgcttcaa aatacttatc
    11101 ctggctatcc attagtaagg ttttaatatt gccaactaaa acggaggctg cctgtgaata
    11161 ctcctgttta aagcgactta actcttttat ggtttcatca aaggtctgcc aggggatgac
    11221 ctgatcgaga tatttattat aaagatccaa tgctccatct gcggtttcga ttgcgttttt
    11281 aactacctct accgttttat ctgcaacgat ttcagtcata atcattcgcc tctttaaata
    11341 tat
//
LOCUS       DAZFDC010000140        11067 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFDC010000140 DAZFDC010000000
VERSION     DAZFDC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.140"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..840
                     /gene="yjiC"
                     /locus_tag="ACHJ48_003990"
     CDS             10..840
                     /gene="yjiC"
                     /locus_tag="ACHJ48_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO4709442.1"
                     /translation="MSMPLSNVLQSQIITDNHFLHHPKVESELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(981..1754)
                     /gene="uxuR"
                     /locus_tag="ACHJ48_003991"
     CDS             complement(981..1754)
                     /gene="uxuR"
                     /locus_tag="ACHJ48_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO4709443.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1969..3429)
                     /gene="uxuB"
                     /locus_tag="ACHJ48_003992"
     CDS             complement(1969..3429)
                     /gene="uxuB"
                     /locus_tag="ACHJ48_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO4709444.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3510..4694)
                     /gene="uxuA"
                     /locus_tag="ACHJ48_003993"
     CDS             complement(3510..4694)
                     /gene="uxuA"
                     /locus_tag="ACHJ48_003993"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO4709445.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SH"
     gene            5034..6377
                     /gene="gntP"
                     /locus_tag="ACHJ48_003994"
     CDS             5034..6377
                     /gene="gntP"
                     /locus_tag="ACHJ48_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO4709446.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6551..7453)
                     /gene="fimH"
                     /locus_tag="ACHJ48_003995"
     CDS             complement(6551..7453)
                     /gene="fimH"
                     /locus_tag="ACHJ48_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO4709447.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTKNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7473..7976)
                     /gene="fimG"
                     /locus_tag="ACHJ48_003996"
     CDS             complement(7473..7976)
                     /gene="fimG"
                     /locus_tag="ACHJ48_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO4709448.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7989..8519)
                     /gene="fimF"
                     /locus_tag="ACHJ48_003997"
     CDS             complement(7989..8519)
                     /gene="fimF"
                     /locus_tag="ACHJ48_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO4709449.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8529..>11067)
                     /gene="fimD"
                     /locus_tag="ACHJ48_003998"
     CDS             complement(8529..>11067)
                     /gene="fimD"
                     /locus_tag="ACHJ48_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO4709450.1"
                     /translation="CAFAVQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPGT
                     YRVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVAGMNLLADDAC
                     VPLTTMVQDATAHLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSG
                     NSVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLE
                     RDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTA
                     QVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPL
                     LQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNF
                     GIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYST
                     SGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSS
                     TLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLARNVN
                     IPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAG
                     GGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLN
                     DTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDN
                     AVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQ
                     VYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2645 a         2867 c         2746 g         2809 t
ORIGIN      
        1 gtaactatta tgtctatgcc attaagcaat gtccttcaga gtcaaattat tacagataat
       61 cacttccttc atcatccaaa agtcgaaagt gagttaacac gtaaatatga acgtgcacgc
      121 ctggatacgg aaaatatcta tcttctgcct cttgctcgtg gcaataacca taattacgat
      181 ggaaaaagcg ttgtcgaaat tagaaaactt gatattagta aagaatcctg gccatttaat
      241 tatgtgacag aagcatgcag agaatccgat ggtattacaa caacaggacg tatgctctat
      301 cgaaacttga aaatcacctc agctttagac gaaatatatg gtggtatatg taaaaaggcg
      361 catgctgcta cagagttagc agaaggttta cggctaaatt tatttatgaa gagccctttc
      421 gatcctgttg aggactatac agttcatgaa ataacattag gccctggttg caatgttcca
      481 ggttacgccg gaaccacgat aggatatatt tcaacccttc cagcttctca ggcaaaaaga
      541 tggaccaatg agcagccaag aattgatatc tatattgatc agataataac tgtctcaggg
      601 gtagcaaatt cttccggatt cgcccttgcc gcattactta atgctaatat tgagttgggt
      661 aatgacccaa taattggtat agaagcctat ccaggtaccg cggagatcca tgcaaaaatg
      721 ggatataaag ttatccccgg tgatgaagat gcgccactca aacgcatgac tctgcaaccg
      781 tcatctcttc cagaactgtt tgaattaaaa aatggtgaat ggaattatat tggcaaataa
      841 ccactgagaa gaatgagtac taacattcag gttatataga tatttaaaca ataccctgca
      901 atttacaaag taatactcat tgcgacttct taatttaaga aagcgcaagg aacgtttacc
      961 cttgcgctta ttataataag tcaggcgtcg actttatcca gcggccatga atcaaacaga
     1021 tagccgtcaa aataaatatc gtcaacgttc gagaattcca gcagacgttg cttaacgttt
     1081 tccagatgct gccacattgc cagcttcgcc gctcgggcat cttttttgat taacgcggcg
     1141 aggatctgtt tgtgatcgcc caaccactct ttgcgataca ggctgtcatc cagatggctg
     1201 tgcaactgga tccacattgg gttgttttcc cgccactgcc aggactgacg gaacagctcc
     1261 accagcatgc tgttatgcgt tgcttcggca atagcgagat ggaactgcat gtcaccgctt
     1321 tcgctgctgc ccggcgcact ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc
     1381 attttgacga tatcttcgcg ggtagcctgc agggcggcaa actcggcgat gttactttcc
     1441 agtaactgac gcgcctgtaa cagctcaaaa ggacccgcat cattgcagac gttggcatcc
     1501 ggactgtctg tgttgtggct gcctgagcta tcaagaacat agataccggc accccggcgc
     1561 acttccacca gccctttgat ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg
     1621 acatcaagca tttctgcaat ttcacgctcc ggcggtagcc gttcaccagg attgtacggc
     1681 gtctttatga tcagatcgcg gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag
     1741 gtggcagatt tcatggtgtt gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc
     1801 ggcgtgaacg ccttatccgg cctacaacgg gcaattttac ggcccccgtt aatctcaaac
     1861 tataccatta gcactggaag ttggcacgta caggtgggca aaaaaacgaa aactggtcaa
     1921 ccaaatatca gtttgattga ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc
     1981 cacacactcg cgcgcaccgc gttcgcacag ctgctgatat gccgcagtca ccgcgccaac
     2041 aaagtcggca ttctgcggca gatcatcggc aaaaataccg ctcaggccca gcagcgcttt
     2101 cacgcggtct gcgccctgat actgcgcgtt aatcttctgg aactcggcca gcatcgggtc
     2161 gaccacgtca atggcattac cctgctcatc cacgccctgg gtgtaacgca tccagccagc
     2221 cacgcccagc gccaggtgac gccagctgcc gccgttttgc aggtgcagac gcaccgggtc
     2281 cagcagacgc tgcggtaact tctggctgcc gtccatcgca atctgccagg tacggtgacg
     2341 cagagacggg ttgctgaaac gctcgatcag cagcgtcgca taggcgttca ggtccgtacc
     2401 ttccggcatc gacagcgttg gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg
     2461 ataatccggg ttagtcatgg tgtcggcaat agtttcatag ccgccgaggt aaccgaggta
     2521 cgccaggaaa gagtggctgc cgttcagcat acgcagtttc atcatttcga acggcacaac
     2581 gtctgcaacg aactgtgcgc ccactttatc ccagtccgga cgaccattaa cgaagttatc
     2641 ttcaataacc cactgacgga acggttcgca ggcaatggcg cacgggtcgt aaacacccag
     2701 ctggtcagca atttcctgta aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat
     2761 ggtgcacggg aaggtgacgt tctcttcaat ccatgccgct agctgcgggt cacgcgcctg
     2821 agccagcccc agtaccgcga ccttcgccac atgaccgttt tcacgcacgt tatcgcagga
     2881 catcaccgta aatgctttca gccctttttc acgacgcaga cgcaaggctt cgacgatgta
     2941 accaatcgcg gacttcggcg cagtcgggtt ttccagatcg tgcttgatca gcgggttatt
     3001 gagatccagt tgaccgcttg ccgcatcagc gcagtagcct ttttccgtga ccgtcaggga
     3061 gacaatcgcc gtttgcggac gcgccatcgc gttgagaata ccttcgcagc cgtcgatttc
     3121 cgggtgcagc gcttctttca tcgaaccgat aattttcagc tcggtgcttt ccgcgccttt
     3181 ttccgccacg gtgtacagca gttgctgttt tttcaggttt tcgatcagca cgcggtcgtt
     3241 gcctggcatc aggttaactt cgcagatacc ccagtcgctg tcggtgcttt ccagcagatg
     3301 atgggtgtac agcgcctggt gcgcacggtg aaacgccccg caaccgagat gcacaatgcg
     3361 tgattccaga cgagaatgat cccatgacgg gcgggcaacc ggcagattgc tgtcaacaat
     3421 agtagtcatc gtaaactcca gaatccgtct tcagagagtg acggcaagga agaaggagcc
     3481 ggcacggcgc gcagccatgc cggtgggtat taatggctaa agaaagcgcg ctggatcgcc
     3541 agttcgacac cgcgaacttc ggccaggcct ttcagacgac caattgcgga gtaaccaggg
     3601 ttggttttct tcttcaggtc gtccagcatc tgatgaccgt ggtccggacg catcgggatc
     3661 aggtcttctt tgccttccgc tttacgacgg tgttcttctt caacaatcgc tttcaccact
     3721 tcgtacatat caacgtcacc gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct
     3781 tcacgcatgg tggagcgcag atgggtgaag taaatacgcg gaccgaactg cttgatcata
     3841 tcaaccaaat cgttgtcagc acgcacgccg taggaaccgg tgcacatggt gaaaccgttc
     3901 gccatgctgt ttacggtatc aaccatccac tgcatatctt caatggtgga aacaatgcgc
     3961 ggcaggccga ggatcgggcg cggcggatcg tccgggtgaa cagccatacg gacgccaact
     4021 tcttcggcaa ccggaataat cgctttcagg aagacagcaa agttttcgcg cagtttggct
     4081 ttgtcgatat ctttgtacag ctccaggtgc ttacggaact ggtcgagggt gtacccttct
     4141 tccgcacctg gcagaccagc gatgatgtta cgggtcagac gcgctttgtc ttcatcgctc
     4201 atagtggcga agcgttcagc tgcctgagca atttcttctt cggtgtaatc cgcttccgcg
     4261 cctggacgtt tcaggatatg catttcgaat gcagcgaatt cgatctggtc gaagcgcaga
     4321 gctttggagc cgtctggcag cacgtattcg aggtcagtac gggtccagtc gagcaccggc
     4381 atgaagttgt agcacacggt gcgaatgccg cactgcgcca ggttgcgcag agtctgctga
     4441 tagttagcga tccactgctc atagttgcca gtgtgggttt tgatatcttc gtgaattggt
     4501 acgctttcta cgacagacca caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg
     4561 atctcttcta cggaccatac ttcgccgttc gggatatggt gcagcgcggt aaccacgcca
     4621 gttgcgcccg cctgacggac atcagctaaa gaaaccggat cgtttgggcc gtaccagcgc
     4681 caggtctgtt ccatgatttt tcctctttca catagtgggt tccacagcaa agagacgcgg
     4741 aaccctggag ttgtttaggt atccggatga acaaccagac cgcagccgtg gcgttgcatc
     4801 ggatgagatg gcgtataagt tctacctttt ggtaagacat gtcaacccga attctgaaat
     4861 tggttaacca catcacaaga atttcactct catggttgtt gctgcaaatt gcagcaaagg
     4921 gtaatcgatg gttattgatc agaggatgtg acattcatcg caacaatggt tgaccaattt
     4981 acataacatg tcgcccaaaa taacaacggt tcaaccaggt aaggtaaaac gttatgcatg
     5041 tgcttaacat tctctgggtg gtattcggca ttggtctgat gctggtactg aatttgaagt
     5101 tcaaaatcaa ttcaatggtg gctttgttgg tggcggcgct gtccgtcggg atgctggcgg
     5161 gcatggattt gatgtcgctg ctgcacacca tgaaagcggg cttcggcaac acgctggggg
     5221 aactggctat catcgtggtg ttcggtgcgg tcatcggtaa attgatggtc gactccggcg
     5281 cggctcacca gatagcgcat acgctgctgg cgcgtctcgg tctgcgctat gtacagctgt
     5341 cggtgattat catcggcctg atttttggtc tggcgatgtt ctatgaagtg gcctttatca
     5401 tgttagcgcc gctggttatt gtcattgccg ccgaagctaa aattccgttc ctgaaactgg
     5461 cgatcccggc agtagcagct gccactaccg cacattcact gttcccaccg cagccgggtc
     5521 cggtggcgct ggtgaatgct tatggcgcag atatggggat ggtttatatc tatggcgtac
     5581 tggtgacgat cccaagtgta atctgcgcag gtctgatcct gccgaagttc ctcggcaatc
     5641 ttgagcgccc aacgccatca ttcctgaaag cagatcaacc ggtagatatg aacaatctgc
     5701 cctctttcgg cgtttcgatt ctggtgccgc tgatcccggc gatcattatg atctctacca
     5761 ccatcgccaa tatctggctg gtaaaagata cccctgcctg ggaagtggtt aactttatcg
     5821 gttcctcgcc gattgcgatg tttattgcga tggtggttgc attcgtactc tttggcaccg
     5881 cgcgtggtca tgacatgcag tgggtgatga acgcttttga aagcgcggtg aagagtattg
     5941 caatggtgat tctgatcatc ggtgcgggtg gcgtgctgaa gcaaactatc atcgacaccg
     6001 gtattggcga caccatcggc atgttgatgt cccacggcaa tatctcgccc tacattatgg
     6061 catggctgat cactgtgcta attcgtctgg cgacgggtca gggtgtcgtt tcggcgatga
     6121 ccgccgccgg gattatcagt gctgcaatcc ttgatccagc aactggtcag ctggttggcg
     6181 tgaatccggc gctgctggtg ctggcgacgg ctgcgggttc caacaccctc acccacatta
     6241 acgatgcatc tttctggttg ttcaaaggtt actttgacct gtcggtaaaa gacacgttga
     6301 aaacctgggg tctgctggag ctggtcaact ccgtggttgg gctgattatt gtgctgatta
     6361 ttagcatggt agcgtaaaag aacaccagag cctgccaatg gcaggctcag ttcggattgt
     6421 cggcaaagtg cgctttgttt atgccagatg cgacgctgac gcgtcttatc tggcctacaa
     6481 agggctaacg tgcaggtttt tagcttcagg taatattgcg tacctgcatt agcaatgccc
     6541 tgtgatttct ttattgataa acaaaagtca cgccaataat cgattgcaca ttccctgcag
     6601 tcacctgccc gccggtacgt gcgtaatttg ccgttaatcc cagacttacc gccgaagttc
     6661 ctactgctcc taacgatacc gtgttattcg ctggaataat cgtaccgttg cgcgtcaact
     6721 gtacgccgac gccctgcgct ggtgaaaacg acgcggtatt ggtgaaaatc gagttgcccg
     6781 catctgcggt tgtgccggag aggtaatacc ccaggttttg gcttttcgca caataaacgg
     6841 taagaggaat tggcactgaa ccagggtagt ccggcagagt aacggtgaca tcacgagcag
     6901 aaacatcgca gccgccagta ggcactacca catcattatt ggcgtaaata ttccacacaa
     6961 actggaaatc atcgctgtta tagtttttgg tctgtcgcaa aataagcacg gcaattaatg
     7021 agccagcttt aatcgccacc ccgcccgcac tgctcacagg cgtcaaataa agcgccaccg
     7081 gccacggctt atccgttctc gaattataaa caacccgcgg cgtttcgctg gtggtcggaa
     7141 atggatagct actgccacta tattttacgg tcccggaaaa attagataac acgccgccat
     7201 aagccgagcc tcgttgcagt gtgacatagt ctgtaatggt ttccggataa tcgttatggc
     7261 aaaagatttg cgtcgaaaga tctacgacca ggttttgccc cacattcacg gcaggcgcaa
     7321 ggtttacata aacattagcg ctgccaccgc caatagggat agcggtacca ttggcggttt
     7381 tacaggcgaa tgaccaggca tttaccgacc agcccatcag cagtacagca aacagggtaa
     7441 taactcgttt cattacaatt atctcttcgg gttcagctgt aggtataggt gatgctaatc
     7501 actgcctgaa tggttccctg agtggctccg ccatttaccg tcaatgctct gacctgtaac
     7561 gggaagtgcg ctgattgtga ggaatcatcc acctgaactg ttttggttgc gccagtattc
     7621 aatgtgttgc cactgtcatc ctgtagctct aactggatgt tttgcgcggt cccctggttt
     7681 ttataatatc cggtactgtc ggctgccccg ctgaagctgg cagtgaccct tgacgttccc
     7741 accggacaat tagtcaactc aagcgcaaca tcatgccagg ccgatgccgc cccggcagac
     7801 atcagactga aagaataaag atcgccgaga tcaaccgtgg cattggtggt ggaaactgtg
     7861 cacggtttgg cgacgacctt accgttcacc gtgatggtga catcggctgc ctgtatcgtc
     7921 gcacttgcga gcgccaatat tgccgccaat aaatacccac gtttgcgcca tttcatgagc
     7981 atctccagtt actgatattc aagagtgaag gtagccgtgg cattgatatg ccccgcagtg
     8041 acaggcacct gtgtcgccat tagccgggcg taaaaattca acgtatttgg tttacccggc
     8101 gtcagggtcg tccacgaaat cgcggacgat ggagcattaa ggggtatctg attttgctgc
     8161 tcattcagaa gctgtattcc cagtcccgaa gccgctgaca ccgtattttc aagtgcaagc
     8221 aggttggcat tgtggctatc tgcaacgccg gtaaacccaa cttttacggc agaaacggcg
     8281 ttaccacagg gtgacagcaa aatacgaaat ggaacgacag gagtcgtcgc gccaatgttg
     8341 ttaaattgct tcgccgcgtt ttccatcaga tcaacagtaa aattggttga ttcagcggcc
     8401 acactacagc cgttatctct gacatagccg cggatagtaa tcgtgctatc cgcagccaaa
     8461 gcgtgactca ctgccagcca caaaaaagcg cacagaagat aaaaaggttt gtttctcatc
     8521 acgccccctt aacgacattc agctgatagc tgggttaata actgctgctg actctctggt
     8581 ggcagttgat aattggcgac acaatgagca ttttcctctt ctccccattt cacctgaact
     8641 tttcccgcta gaggcattcc gctgaggtaa acctgaccat tatccgcaac aatgccgcta
     8701 ctctggctac tctctgatgt caccatcgcc ccaaacggca gcggcttatt attgtgggtt
     8761 agcgtcatga gcagttttat cccaacgcgc gctttaaact ctgctcgcac gatcgcccca
     8821 cgagtgggaa caacgttagc gaccgcgtta tctaaatcga cgttatcagc cagggtattg
     8881 gtatccagcg ccactctatt ttcccgatat tcagtggcat aaggcagcac ggcataaccg
     8941 cgccagtcgg tacgcacccc cgtctggttt tcgacttttg catcttttgc gccaggcgct
     9001 ttaacaagca ccaccgtatc gtttaacggc tgccccagcg ttacgccatt ggcatgagcc
     9061 agtaccccac cgctgactcc gtaatagagc tgcttaatat catcgctatg gctgtaaccg
     9121 atattggcat tgccgtaacc accgcgataa ttcagcgtgg cgtagcctgt gcttccgcta
     9181 ttaccatcgc ctcccccggc atagccggtt tgcacgctat agctgaggtt gttgtcttcc
     9241 agcaacgtac cgtatacacc agccagattg gtcatccgac cgttgagatc gtgtgacatg
     9301 ctgtagctgg cactggcatg tcgccactga gatttactgt cagaacgcag ccagtggctg
     9361 aaaggaatat tgacgttacg cgctaacatc tgatcacgtc ctttttgcca ggcgtttttc
     9421 gtcaggctat agctgagcgt ccagttgata tcttcgaacg cagtatttaa tccagcctgg
     9481 aattgctcat cgacattact cgttccccaa taagtttgat ggctaccact caaatacagt
     9541 gttgatgagc gcccgagttg ctgagtaacg gtgagttgta atttcccgcg tttgttataa
     9601 gcgaggttgt aatagtcggt gaatttcggc ttaacctgaa taactccgtc ctgtgtttcg
     9661 atgttgtagc cattcattcg actgtatgtt gtatcagcga aattaaaata tccgctggtc
     9721 gaataacggt aacccactaa ctgaatattc gtgcctgact cattgagcga tttgttatag
     9781 agaaaacgca ccgattgtcc gtcatgctga ctgtcatcgg gaagtgtgga attagcctga
     9841 gtcatatcca cagacagagc gcccagtgcc cccatatttt tcccgatacc aaaattaaaa
     9901 gcacgataac gatctgccag ttgcgttcca ccatatattg tccagccagc tggaaggccg
     9961 tggagtaatg tactttggaa aaagcggggt ttttcctgtt gcgcatttcc actacggtat
    10021 tctcctgccg taatggaata acgagtatgc ccttcacgtt gcaaaagcgg gactgacgaa
    10081 tagggtacgg taaaaatctg cgtgctgccg tcagcctctt taatcgttac ctgcaagtca
    10141 ccactattac ctgcggcata gatatcgttg atggtaaaag gccccggcgg caccgtacta
    10201 ttataaatgt catacccatt ttgtttaata gtgacctgtg cagtaccacg agcaataccg
    10261 tggatcaccg gggcaaatcc tctttggcta tcgggtaaca tattgtcatc tgaggccaat
    10321 tgtgcgccgc gaaagttaat accatcgaaa atatcaccct gagtataacc atcacccagc
    10381 gtcagccggg aacgtaacgg tattatgtct cgctcaagcc aggtattgat atgctgccat
    10441 ttatttttgc tacctgatga tctgtcgcta ctgttataac tccaggtggt attgtcgcgt
    10501 aaacgccacg caccaatatt taacccactc tgtaggttta aatatgcata atggctgtta
    10561 cccccaatcc gattctgtac actatttccg ctgaaattat aattgagcaa tccggcatta
    10621 ataccgggat cccataactc aggaggaata taaccacgcg cgcgattact cataaatgcc
    10681 tgagggatcg tcaggttcag tcgctgctga ccaacatcta aatgcgcagt agcgtcctgg
    10741 accattgtgg ttaatggcac acaggcatca tccgccagca gattcatacc ggcgacagaa
    10801 gccgtattca gccccatact ggcgagttgc gcgcgtgtca ggcagggaac aatcccttgt
    10861 tcactgtcgc ccgtattaaa tgtgacatca cgcgttgcca tataaccatt attcaaatag
    10921 atatcgacgc gatacgtccc tggcggtaat tcttgcccat tttcaaaacg cgataaatcg
    10981 gccacagcct ggggatcatc cgctaaaaag cgcggattaa aatagagttc ggcagatgac
    11041 aaaggtgcct gtacggcaaa agcacag
//
LOCUS       DAZFDC010000141        10964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFDC010000141 DAZFDC010000000
VERSION     DAZFDC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.141"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..108
                     /locus_tag="ACHJ48_003999"
     CDS             <1..108
                     /locus_tag="ACHJ48_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4709451.1"
                     /translation="FLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            87..191
                     /gene="speFL"
                     /locus_tag="ACHJ48_004000"
     CDS             87..191
                     /gene="speFL"
                     /locus_tag="ACHJ48_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO4709452.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            502..2700
                     /gene="speF"
                     /locus_tag="ACHJ48_004001"
     CDS             502..2700
                     /gene="speF"
                     /locus_tag="ACHJ48_004001"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO4709453.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFIPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2697..4016
                     /gene="potE"
                     /locus_tag="ACHJ48_004002"
     CDS             2697..4016
                     /gene="potE"
                     /locus_tag="ACHJ48_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO4709454.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSHVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4073..5713)
                     /gene="pgm"
                     /locus_tag="ACHJ48_004003"
     CDS             complement(4073..5713)
                     /gene="pgm"
                     /locus_tag="ACHJ48_004003"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO4709455.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKAVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5739..6284)
                     /gene="seqA"
                     /locus_tag="ACHJ48_004004"
     CDS             complement(5739..6284)
                     /gene="seqA"
                     /locus_tag="ACHJ48_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO4709456.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPIKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6469..7233
                     /gene="ybfF"
                     /locus_tag="ACHJ48_004005"
     CDS             6469..7233
                     /gene="ybfF"
                     /locus_tag="ACHJ48_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO4709457.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIAHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7373..7666
                     /gene="ybfE"
                     /locus_tag="ACHJ48_004006"
     CDS             7373..7666
                     /gene="ybfE"
                     /locus_tag="ACHJ48_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO4709458.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7806..8336
                     /gene="fldA"
                     /locus_tag="ACHJ48_004007"
     CDS             7806..8336
                     /gene="fldA"
                     /locus_tag="ACHJ48_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO4709459.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8546..8632
                     /gene="uof"
                     /locus_tag="ACHJ48_004008"
     CDS             8546..8632
                     /gene="uof"
                     /locus_tag="ACHJ48_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO4709460.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8625..9071
                     /gene="fur"
                     /locus_tag="ACHJ48_004009"
     CDS             8625..9071
                     /gene="fur"
                     /locus_tag="ACHJ48_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO4709461.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9155..9481)
                     /gene="chiQ"
                     /locus_tag="ACHJ48_004010"
     CDS             complement(9155..9481)
                     /gene="chiQ"
                     /locus_tag="ACHJ48_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO4709462.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9531..10936)
                     /gene="chiP"
                     /locus_tag="ACHJ48_004011"
                     /pseudo
     CDS             complement(9531..10936)
                     /gene="chiP"
                     /locus_tag="ACHJ48_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2640 a         2730 c         2740 g         2854 t
ORIGIN      
        1 tttttgttga tcttcttcac agattatagc catttcatgg atagaataac tctaccttca
       61 actgacacag caagaggtaa aggtaaatgg aaaataataa ccgcttaatg cctcatataa
      121 ggcggacaac ccatatcatg aagtttgccc atcgtaatag ttttgacttt catttcttta
      181 atgcccgcta gtcttctgac taaagggcac cccagcgtac aggtctccct gactttaagc
      241 attacaggtt aatacctgta tccctcggtg ctcatatact gctaaccctt ttaaactcta
      301 cataattcga gtcgcagcat atgcaacttg aagtatgacg agtatagcca gttaccgggc
      361 tggtctgggt tattgcatct gcaaaaagca aactactgat ttatttatca gcggtggagc
      421 tttgcttttt ttccggcgtg atcgatttct cctttgagaa attgaggacc tgctattacc
      481 tgaaataaag agatgaacaa aatgtcagaa ttaaaaattg ccgttagtcg ttcttgcccg
      541 gattgttttt ccactcatcg tgcatgcgtg aatatagacg aaagtaatta tattgacgtt
      601 gccgccatta ttttatcagt cagtgatgtt gaacgtggaa aactcgatga aatagacgct
      661 actggctatg acattcctgt ttttattgca acggaaaatg aagaacgtat accagcagaa
      721 tatctttcac gtatttctgg tgtcttcgaa catggagaag cacgcaaaga attttatggt
      781 cgtcagttag aaaccgctgc cagccattat gaaactcaac tgcgcccacc tttctttcgc
      841 gcactggtcg attatgtcaa tcagggcaac agcgcatttg actgccccgg tcatcagggc
      901 ggcgaatttt tccgtcgcca tccggcgggg aatcagtttg tggaatactt tggtgaggcg
      961 ctgttccgtg ccgacttgtg caacgccgac gtagcgatgg gcgatctgct gattcacgaa
     1021 ggcgcgccat gcattgcaca gcaacatgca gcaaaagtgt ttaatgccga taaaacctac
     1081 ttcgttttaa atggcacttc atcttctaac aaagtggttt taaacgccct gctgacaccg
     1141 ggtgatctgg tgctatttga ccgcaataac cataagtcta accaccacgg ggccttgcta
     1201 caggctggtg caacaccggt ttatctggaa acagcacgta acccgtatgg ttttatcggt
     1261 ggtattgatg cacactgctt cgaagaaagt tatctgcgtg aactgattac agaagttgca
     1321 ccgcaacggg caaaagaggc gcgtccgttc cgcctcgctg tgattcagct aggcacctac
     1381 gacggtacga tttataacgc ccgccaggtg gtggataaaa ttggtcatct gtgtgactac
     1441 atcctgtttg attccgcatg ggtcggctat gaacagttta ttccgatgat ggctgactgt
     1501 tcgccgctgt tgctggatct taatgagaac gatccgggta ttctggttac gcaatccgta
     1561 cataaacagc aggctggttt ttctcaaact tcacaaatcc ataaaaaaga cagccacatc
     1621 aaagggcaac agcgttatgt accgcacaaa cgcatgaaca acgcctttat gatgcatgcc
     1681 tccaccagcc cgttctatcc gctgtttgcc gcactggata tcaacgccaa aatgcatgaa
     1741 ggtgtcagcg gtcgtaatat gtggatggat tgtgtggtaa atggcattaa tgcccgcaaa
     1801 ctgatcctcg ataactgtca gcatattcgt ccgttcatac ctgaactggt ggatggtaaa
     1861 ccctggcagt cgtatgaaac agcgcaaatt gcggttgatc tgcgcttctt ccagtttgta
     1921 ccaggtgagc actggcattc ttttgaaggc tatgcagaga atcaatactt tgttgatccg
     1981 tgcaaactgt tgctgacaac cccaggtatt gatgcacgta acggcgaata tgaagcgttc
     2041 ggtgtacccg cgacgattct tgctaacttc ctgcgcgaaa atggcgtagt gccggaaaaa
     2101 tgcgatctta actccatcct cttcctgctg actccggcag aagatatggc caaacttcag
     2161 caacttgttg ccctgctggt acgcttcgaa aaactgctgg aatctgatgc gccgctggcg
     2221 gaagtgctac cttccatcta caaacagcat gaagagcgct acgccggtta tacccttcgt
     2281 cagttgtgcc aggaaatgca tgatttgtat gcccgccaca acgtgaaaca actgcaaaaa
     2341 gagatgttcc gtaaggaaca cttcccacgc gtcagcatga atccgcaaga agctaactac
     2401 gcctatttac gcggtgaagt ggagctggtt cgtctgccgg atgcagaagg ccgtatcgct
     2461 gccgaaggtg cgcttcctta tcctccgggt gtgctgtgtg ttgttccggg tgaaatctgg
     2521 ggtggtgctg ttttgcgtta cttcagcgct ctggaagaag ggatcaacct gctgccaggt
     2581 tttgcaccgg agctgcaggg tgtctatatc gaagaacatg atggtcgtaa gcaagtttgg
     2641 tgctatgtca tcaagcctcg tgatgcgcaa agcaccctgc tgaaagggga aaaattatga
     2701 gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac catactgacg atggtcaaca
     2761 tgatgggctc cggtatcatc atgctgccga caaagcttgc cgaagttggg acaatctcaa
     2821 ttatctcctg gctggtgaca gccgtaggct caatggcact ggcatgggca ttcgcaaaat
     2881 gcggtatgtt cagccgtaaa tcaggcggta tgggcggtta tgccgagtat gctttcggta
     2941 aatccggtaa ctttatggcg aactatacct acggcgtctc actgctgatt gctaacgtcg
     3001 cgattgctat ttcggcggtt ggttacggca ccgaactgct cggcgcgagt ttgtcgccag
     3061 tgcagattgg tcttgcgacc atcggggtgc tgtggatttg taccgtggct aactttggtg
     3121 gtgcgcgcat taccgggcaa atcagtagca ttactgtgtg gggagtcatt attccggtcg
     3181 tcggtctgtg cattattggc tggttctggt ttagcccgac gctgtacgtt gattcctgga
     3241 atccgcatca tgcaccgttc ttcagtgcgg taggttcttc catcgctatg acgctgtggg
     3301 cttttcttgg tctggagtct gcgtgtgcaa atactgatgt agtggaaaac ccggaacgta
     3361 atgtgccaat cgcggtactc ggcggtacgt taggtgcggc ggtgatttat atcgtctcca
     3421 ccaacgtgat tgccgggatt gtgccaaata tggagctggc aaattcaacg gcaccatttg
     3481 gtctggcctt cgcgcagatg ttcacgccgg aagtgggtaa agtcattatg gcgctgatgg
     3541 tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac cattgcccag gtgtttaaat
     3601 cttcatctga tgaaggctac ttccctaaaa ttttctccca tgtaaccaaa gtggatgcac
     3661 cggtgcaggg aatgttgacc attgtgatta tccagagcgg gttggcgctg atgacgatta
     3721 gcccgtcgct aaacagtcag ttcaacgtgc tggttaacct ggccgtggtg accaatatca
     3781 ttccgtatat tctgtcaatg gcggcattag tcattattca gaaggtcgct aatgtgcccc
     3841 cctcaaaagc gaaagttgca aactttgttg cttttgttgg cgcgatgtac agcttttatg
     3901 cgctgtactc atccggggaa gaagccatgc tgtacggttc catcgtgacc ttccttggtt
     3961 ggacactgta tggtctggta tcaccacgct ttgaactgaa aaataaacac ggttaaaaaa
     4021 gcaaagtaaa aaagggcgat cttgcgaccg ccctttttct tatttaatgt gtttacgcgt
     4081 ttttcagaac ttcgctaaca atctcaaccg cttctttctc aatctgcttg cgatgttctt
     4141 caccgaggaa gctttcgcag tagatcttat atgcgtcttc cgtgcctgac ggacgcgcgg
     4201 cgaaccagcc gttgtcagtc atcactttca gaccgccaat agaagcaccg ttgcccggag
     4261 cagcagtcag gcgcgcggtg atcgggtcac cagccagggt gctggcactc accatttccg
     4321 gagacagctt agacagcgcc gctttttgtg cggaagtcgc agctgcctgc aaacggttgt
     4381 agctcggcgc accaaagcgt tttgccagtt cgttgtagtg ttcctgcggg ttcttaccgg
     4441 tgacagcggt gatttccgcc gccagcagac acatgatgat gccgtctttg tcggtggacc
     4501 acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct ctcttcgccg ccaaagccga
     4561 agctgccgtc gaacagacca tcaacaaacc atttgaaacc taccgggact tctaccagtt
     4621 tacggcccaa gtcgttgacc acacggtcga tcatcgcaga tgaaaccagc gttttaccga
     4681 cggcaacagc tttgccccac tgcggacgat gctggaacag gtagttgatt gccaccgcca
     4741 ggtagtggtt cggattcatc aaacctgccg gagtgacgat accgtgacgg tcataatccg
     4801 ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc cagcaggccc gccatcgcac
     4861 actcggagga gcagtccata cggatcgcgc cgtctttatc aaggtgcata aagcggaagg
     4921 tttgatcgac ctgatcgtta acgatggtca ggttgaggtt gtaatactcg ccaatacgct
     4981 tccagtattc gataccggaa ccgcccagcg gatcaacgcc aagcgtcagg cccgctttct
     5041 gaatcgccgc catatcaacg atatcggcca gcccttccac aaacggctgc accagatcct
     5101 gctctttcac atgaccggat gccatcgctt cgtcgaggga gatacgcttc acgcctttca
     5161 ggccatcggc catcagtgcg ttggctctgt cttccaccac tttagtgacg ttggtatcag
     5221 ccgggccacc atttggcgga ttgtacttga taccaccatc ttccggcggg ttatgggacg
     5281 gtgtaataac gataccgtct gccagcgggc cacctttttt attgtgaacc aggatggcat
     5341 tggaaacggc aggcgtcggg gtgaagccat tgttttcctg cacaataaca tctacgccgt
     5401 tcgctgccag cacttccaga acggaaataa atgcaggttc ggacagggcg tgagtatctt
     5461 tacccacata gcaagggcca gtgatgccgt ttttcgcacg ttcttcagca attgcctgag
     5521 cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc tgcactgcca cggtgaccgg
     5581 aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc tggtttcagt acataatatt
     5641 gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg tgcaggttgg cctgcacgat
     5701 tgtggattgc cattgctttg tcctttgtct gcaacgtttt agatagttcc gcaaaccttc
     5761 tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt gttcgatcat gctgcattta
     5821 cggccggtgt tggtgttggt gatcacccaa tacggcgtgc ctggcacatg tttcggcttg
     5881 gtctgattac catttttcag cagcgtttgt tcatctgccg caaagtaaac gcgtgtacga
     5941 ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt caagagaata tagtgtagac
     6001 aacagcagca taaagcgatt gaccgctcgc ttttgctctg catattcatc cgaaagcaga
     6061 agttcacgca ttgcgcgaac cttgtcttta atcgttttga tcggcttcgc ttcgacgata
     6121 gcaggtgacg caacgcgaac ctctttcgtc accggagcag caggctgtga tgcggcggaa
     6181 aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc cgatatgctt agtgtggctg
     6241 gcaatatagc tgtagagttc atcatcaact tcaatcgttt tcatcttaat ccagtgcagt
     6301 gtcttagctg aatacaagtc gccaggagta tatgaataaa tcccggcagc ggatagcgtc
     6361 aagcctggct tgcagaaaaa ctcggaataa accttatttg ttgcagccct gtggcacaat
     6421 ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga actttgccat gaaattgaat
     6481 atccgcgcgc aaactgcaca aaaccagcac aataattctc ccatcgttct tgtccatggt
     6541 ctgtttggca gcctcgacaa ccttggcgta ctggctcgcg atctggtaaa cgatcacaat
     6601 atcatccagg ttgatatgcg taaccacggt ctttcaccga gagatccagt aatgaattac
     6661 ccggcgatgg cgcaggatct tgttgatact ctggatgcac agcagatcga caaagcaaca
     6721 tttatcgctc actccatggg cggtaaagcg gtaatggcac ttactgcact ggcccccgat
     6781 cgcatcgata aactggtggc gatcgatatc gcaccggtcg actatcacgt acgccgtcat
     6841 gatgagattt tctcggctat caacgcggtc agtgaatcgg acgcacaaac tcgccagcaa
     6901 gcagcagcaa taatgcgcca gcatcttaat gaagaagggg tgattcagtt tctgttgaaa
     6961 tcttttgttg acggggagtg gcgctttaac gtgccggtat tgtgggatca gtatccgcat
     7021 attgtgggtt gggagaaaat cccggcatgg gatcaccctg ccctgtttat ccctggtggt
     7081 aattctccgt atgttagcga gcagtaccgt gatgatttac tggcacaatt tccacaggca
     7141 cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg ctgaaaaacc ggatgcggta
     7201 ttacgcgcta tccgtcgcta tctcaatgat taacgcaact gattaaaaac ccagcgtccg
     7261 cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg ctgatgtatt atggcgcgct
     7321 atccatccgg gcagaggcct ggctgattgt ttcccccgaa gtcaccaaga tcatggccaa
     7381 agaacaaacg gaccgtacga cattagatct gttcgcgcac gagcgtcgac cgggacgacc
     7441 gaaaacaaat ccgctttcgc gcgatgaaca gctgcgtatt aataaacgca accaactaaa
     7501 acgcgacaaa gtacgtggcc taaagcgtgt cgaactgaag ctgaacgcgg aagctgtcga
     7561 ggcgctgaac gagctggcgg aatcgcgcaa tatgagccgt agcgaactga tcgaagagat
     7621 gttaatgcag caactggcgg cgctgcgtag tcagggcatc gtttaaattt ccactttcat
     7681 gtagcacagt gtgcagtcct gctcgtttgc tgattccgca agactgcctg ttctgctatg
     7741 attgccttta tccgtgggca attttccacc cccatttcaa taagtttcaa gaggttattt
     7801 cactcatggc tatcactggc atctttttcg gcagcgacac cggtaatacc gaaaatatcg
     7861 caaaaatgat tcaaaaacag cttggtaaag acgttgccga tgtccatgac attgcaaaaa
     7921 gcagcaaaga agatctggaa gcttatgaca ttctgctgct gggcatccca acctggtatt
     7981 acggcgaagc gcagtgtgac tgggatgact tcttcccgac tctcgaagag attgatttca
     8041 atggcaaact ggttgcgctg tttggttgtg gtgaccagga agattacgcc gaatatttct
     8101 gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg cggtgcaacc atcgttggtc
     8161 actggccaac tgcgggctat catttcgaag catcaaaagg tctggcagat gacgaccact
     8221 ttgtcggtct ggctatcgac gaagaccgtc agccggaact gaccgctgaa cgtgtagaaa
     8281 aatgggttaa acagatttct gaagagttgc atctcgacga aattctcaat gcctgatgtg
     8341 atgcggcgta gactcatgtc tacgccgtat taatagataa tgccaatcaa aataattgct
     8401 acaaatttgt aacttttgcc gttgtacctg tacaatgtcc cggtgttcaa gtggccttgc
     8461 cgttgtaaat gtaagctgtg ccacgttttt attaacaata tttgccaggg acttgtggtt
     8521 ttcatttagg cgtggcaatt ctataatgat acgcattatc tcaagagcaa attctgtcac
     8581 ttcttctaat gaagtgaacc gcttagtaac aggacagatt ccgcatgact gataacaata
     8641 ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg tttaaaaatc ctggaagttc
     8701 ttcaggagcc ggacaaccat cacgtcagtg cggaagattt atacaaacgt ctgatcgata
     8761 tgggtgaaga aattggtctg gctacggtat atcgcgtact gaaccagttt gacgacgctg
     8821 gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt atttgaactg acacagcaac
     8881 atcaccacga tcacctgatc tgcctcgact gcggcaaggt tatcgaattt agtgatgatt
     8941 ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg cattcgcctg actaaccaca
     9001 gtctctatct ttacggtcac tgcgccgaag gcgattgccg cgaagatgag cacgcgcacg
     9061 aaggcaaata agccagtctg aacgagaaaa gccaacctgc gggttggctt ttttatgcaa
     9121 gggaaataag aaaagcgaga gttacagctc tcacttattt gttattactg cgaatttcct
     9181 gccagacctt atcacaatcg gctttcaccg cctgatcatt tccggtttgc gttgcacgca
     9241 gacactgctg ataatccagt acgcggacac tttcctggtc ggcaaactgc tgatgttgtt
     9301 tctctttctt cagcacgttt aacacgctct ggcaggcttc aattttttcc ggcgaacctt
     9361 gtgcggtgtt gatacaggcg ctgtatgcct ctttcagacg gctgtcttcc tgtggcgcgg
     9421 ttgattgcgc acaagctacc agccccgatg ccatcatggc gatgagaatc agttttttca
     9481 tagcaaattc ctcaaacgtg cggcaggcac aaaacctgcc gcgtcgggca tcagaagatg
     9541 gtgaatggtg cgattaccat aaattttacg tcacgctcat cctggaagat gttgccgtaa
     9601 ccgccgcccc agcttgggat gtcggagtgg ttgtcgtatt cggtgaagtg cagtttgaac
     9661 atcgtgcctt tggcgcgacc gtcctgaatg gtgtagaccg catccaggct gtatgcagac
     9721 tcttcaatag tccggttttt gtcgtagtac gcatccggat tgctctgcca ggtcgcaggt
     9781 ttagcatccc atgcgtaaac gtaggaagcg ccgatggcga agcctggaag attccagttt
     9841 ttcaggtcat acatcgcacc gaagaagacc gctttttcgc cgttggcgtt gaagtcagaa
     9901 cggttatccc accagatatc caggcgaccg ttcgaggaag cgtaggttgg agtcatacgt
     9961 tgcaggaagt atccctgctg accgtcagct ttaacccagg tgccttcgag gcgcaaatcc
    10021 actacgtcag ccgcccggta accaaaggtc aacgcttgca gccaggcggt gccgtcataa
    10081 agatcgttga cgctgcgatc gtcaacttta tcgcgcgtac cgtagaactg gtagctggtg
    10141 gttaacgggc taccggcgat atcaaatttg tagctggctt tggcaaagta ttgatcgata
    10201 tacccttccg cctgaccaaa tgccgcttcc agtacgaagt tatttttgaa gtcgtatttc
    10261 gccccaaggg agtgcagata atcaactttg gtggttttat cgttctgata aaactcatcc
    10321 atttccagat gccacggtgc tttgtattcg ttggtccaca tgtaggagaa actcaacgca
    10381 ccagcatcgc cgtaatcaaa attcgccccg gcctccgcac cctgataagt acccggcata
    10441 aagctccagt gcggcgctaa cagcgtttga ccagttggct gaatataacc tgccctcgcc
    10501 acaccggacc gtatttaaat ttggccgctg ctttatacag gcttataccg cttttatcgc
    10561 cggaccagtc ttcgtcatag gctttattac tttttgaaaa cgcgatttcg ttcgggtgag
    10621 agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc ggcaatatca aggccgaaca
    10681 tatcagcagc ataaccggac tgaaaatcga ggttggcgtt ccaggtggag tgagaaaggt
    10741 tggttttgta tttatcgccg tcggtaacat ctttacggtc gcgttcacgc tgccagtaat
    10801 agataccgcc ggttaaggtt gaatcgtcaa tgaatccttc ggcgcgagcc tccggcattg
    10861 ccataaagcc cgacattgct gtaacaccgg cgatagccag cgccagcgta ctacgtttgc
    10921 cactaaacgt acgcatgggt taatcctctt tgacgtataa attg
//
LOCUS       DAZFDC010000142        10909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFDC010000142 DAZFDC010000000
VERSION     DAZFDC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.142"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            67..828
                     /gene="adiY"
                     /locus_tag="ACHJ48_004012"
     CDS             67..828
                     /gene="adiY"
                     /locus_tag="ACHJ48_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO4709463.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            965..2302
                     /gene="adiC"
                     /locus_tag="ACHJ48_004013"
     CDS             965..2302
                     /gene="adiC"
                     /locus_tag="ACHJ48_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO4709464.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2406..4049
                     /gene="eptA"
                     /locus_tag="ACHJ48_004014"
     CDS             2406..4049
                     /gene="eptA"
                     /locus_tag="ACHJ48_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO4709465.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYKVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4046..4714
                     /gene="basR"
                     /locus_tag="ACHJ48_004015"
     CDS             4046..4714
                     /gene="basR"
                     /locus_tag="ACHJ48_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO4709466.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4715..5815
                     /gene="pmrB"
                     /locus_tag="ACHJ48_004016"
     CDS             4715..5815
                     /gene="pmrB"
                     /locus_tag="ACHJ48_004016"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO4709467.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5791..5880)
                     /gene="pmrR"
                     /locus_tag="ACHJ48_004017"
     CDS             complement(5791..5880)
                     /gene="pmrR"
                     /locus_tag="ACHJ48_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO4709468.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5992..7494)
                     /gene="proP"
                     /locus_tag="ACHJ48_004018"
     CDS             complement(5992..7494)
                     /gene="proP"
                     /locus_tag="ACHJ48_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO4709469.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7758..8636)
                     /gene="rdcB"
                     /locus_tag="ACHJ48_004019"
     CDS             complement(7758..8636)
                     /gene="rdcB"
                     /locus_tag="ACHJ48_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO4709470.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8633..10861)
                     /gene="crfC"
                     /locus_tag="ACHJ48_004020"
     CDS             complement(8633..10861)
                     /gene="crfC"
                     /locus_tag="ACHJ48_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO4709471.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2727 a         2861 c         2690 g         2631 t
ORIGIN      
        1 accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc actcaggagt
       61 acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga aaaagatgtc
      121 tggataaggg ttaatgggaa agaacctatt agccttaaag ctaaccatat ggcgttatta
      181 aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt tgctcatatt
      241 agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc gcaaatacca
      301 gtatggcaac gtagcgctac gcccattctc accctgccat gcctgacacc agacgtcttt
      361 cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa ggaacgaaca
      421 cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt cctttcatta
      481 atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat tgaaagcgat
      541 attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag cccaagtctg
      601 ttaaagaaaa agctgaaaag cgaaaacacc agttatagcc aaataatcac cacctgccgc
      661 atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca ggtatcacag
      721 tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt ctacggtatg
      781 acaccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt tttaacctta
      841 acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa ttcaactatc
      901 gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc gcaggagttt
      961 aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca ccctgatggt
     1021 gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg cctccaccgg
     1081 cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc tctcgatggt
     1141 atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt acgcccgccg
     1201 ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg cctgctggat
     1261 cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct tcccgattct
     1321 gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct tcgtcctgct
     1381 gaacattgtc ggtccgaaaa tgatcacccg tgtgcaggcc gttgccaccg tgctggcgct
     1441 gatccccatc gtcgggattg ccgtatttgg ctggttctgg tttcgtggtg aaacctatat
     1501 ggcggcatgg aacgtcagcg gcctgggcac cttcggtgca attcaaagta cccttaacgt
     1561 tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg tggtgaaaaa
     1621 cccgaaacgc aacgtcccta tagccaccat tggtggggta ttgattgccg ccgtttgcta
     1681 tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc gcgtttctgc
     1741 ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgccgggg ccatcgtttc
     1801 cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacattgc tggcgggtca
     1861 aacggcgaaa gcggctgccg atgacggact gttcccaccg atttttgccc gtgtaaataa
     1921 agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca tcttccagct
     1981 cagcagcatt tcaccaaacg cgaccaaaga attcggtctg gtttcttccg tgtcggtcat
     2041 ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg gacacggtca
     2101 ctttggtaaa gcacgcccgg catatctggc ggttactacc attgccttcc tctactgcat
     2161 ctgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca ccctgatggt
     2221 catcaccgcc atgtatgcgc tgaattacaa ccggctgcat aaaaacccgt atcccttaga
     2281 tgcaccaata agcaaagatt aattcccctt aatccagcaa agataaaagc caaccttaag
     2341 aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc gatggaaaca
     2401 ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc tggctattgt
     2461 tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag gtgttgcagg
     2521 cgctgccgct ggactcgctg cataacgtac tggttttctt gtcgatgccg gtcgtagcct
     2581 tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat cgaccgctgg
     2641 cctgcctgtt tattctggtt ggcgcggctg cacaatattt cataatgact tacggcatcg
     2701 tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccggcagaa agctatgcgc
     2761 tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct gcgctgattg
     2821 cctgctggat taaaatcaaa cctgccacct cgcgtctgcg cagtgttctt ttccgtggag
     2881 ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactattt tataaagact
     2941 acgcctcgtt gttccgcaac aacaaagagc tggtgaagtc cttaagcccc tctaacagca
     3001 ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg ctggtgcgaa
     3061 ttggtgaaga cgcgcaccgc aacccattaa tgcagaacga aaaacgtaaa aatttgacca
     3121 tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc tacccgcgtg
     3181 aaactaaccc gcggttggcg aaagataacg tggtctattt ccctaatacc gcatcttgcg
     3241 gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcga gagcactaca
     3301 aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga gcgggcatca
     3361 acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc gtgcctcacc
     3421 agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc tatgacgaag
     3481 tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc gtgattgtct
     3541 tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg cagttcagga
     3601 aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag caactggtga
     3661 acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg attaatctgc
     3721 tgaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac cacggtgaat
     3781 cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc ccggatagcc
     3841 aaaaacaggt gccgatgctg ctgtggctgt cggaggatta tcaaaaacgg tataaggttg
     3901 accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac aatttattct
     3961 ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggctgcg gatgatattc
     4021 tgcaaacttg caggagagtg agtgaatgaa aattctgatt gttgaagacg atacgctgtt
     4081 attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg gcgtgacaac
     4141 cgcgcggatg gcggaacaaa gccttgaggc aggtcattac agcctggtgg tactggattt
     4201 agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga aaaaatatac
     4261 cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg ccgggctgga
     4321 tgtcggtgcc gacgactatc tggtgaagcc ttttgcgctg gaagagttac atgcccgtat
     4381 ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg ttggcaatct
     4441 gacgctgaac atgggtcgcc gtcaggtatg gatgggcggt gaagagttga ttctgacgcc
     4501 caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg tgcatcggga
     4561 aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca ccctggaagt
     4621 gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg tgcgcggctt
     4681 tggctatatg cttgtcgcga atgaggaaaa ctaattgaat ctgatgcgtt ttctgcgccg
     4741 accaatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt tggtgtttga
     4801 gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc tgtttgagca
     4861 ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc gcgaggcggt
     4921 cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta tctgctatca
     4981 ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg aagcgcgcac
     5041 cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg aagcggtggt
     5101 ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg aaaggttatt
     5161 taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt tgcatctgga
     5221 actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc ttgatcagat
     5281 gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat tttcttccgg
     5341 taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg acgaactcag
     5401 taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg ccgacatcac
     5461 cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag aaaacgccca
     5521 tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg gcggagcggt
     5581 catggccgtt gaagatgaag gaccgggtat tgatgagagt aaatgcgggg agttgagtaa
     5641 agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa gtattgtcag
     5701 ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag agacttccgg
     5761 cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga tataaagaaa
     5821 gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac ggtttttcat
     5881 tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag tgaaccttaa
     5941 gagttcaatt ggcctgataa gacagcgtta catcaggcca tccgtttcag cttattcatc
     6001 aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg caatctcgtg
     6061 gtcaatatca tcgattttct gctcgatatt atcgtaatgc tcgacgagaa tttccttcgc
     6121 ttcctgtata tctgacgccg ccggtgttgc acctttcaac ggacgatttg ccgtctcttt
     6181 catggttacg ccggtgatta aaccaaccac cgccactacc atcaggtaat aggctggcat
     6241 catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca gaccggcaac
     6301 cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg tcgggaacat
     6361 cgctggcaag gtagaagcca taacgcccgt aaagcagtta aggatcaccg ccagcatcag
     6421 taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg ccgggatcgc
     6481 caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa aacggtcact
     6541 cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga taataatcag
     6601 caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca tataggtcag
     6661 caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca acaggctgcg
     6721 ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca aaccttcgcg
     6781 gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt ccagcgcatg
     6841 gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa tacgccagcc
     6901 ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca cgcccgcacc
     6961 cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc cacgtttacg
     7021 gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccgc cgaccgagaa
     7081 accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa tcgtgtcgta
     7141 ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag tgatagcgag
     7201 gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc caagcggtcg
     7261 aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc tggggtcagc
     7321 ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac caaaatcaaa
     7381 ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt taccgtcatc
     7441 aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca gcatagcttt
     7501 cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg gccgcaaaag
     7561 gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg cttacaccct
     7621 ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt aatgaccttt
     7681 ttaccctacc agcgtttaaa tttgtgatca acttcacaca tggatttcgt ttaggactca
     7741 ttgatgtaac tgattattta caccaaattt acagagaaga tttcctcgag cattgccccc
     7801 gccagacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg ccagcgttgg
     7861 gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag ccaacttaaa
     7921 aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg atggtaatag
     7981 tgacgcgtgg tcggggccgc attgatactc tctgccagat gttgtgtcgc tttcacgcgc
     8041 aaatcaccca gcaacaacgc caccgcttgc gggcttccca gacgacaggc atcgccaaat
     8101 gccccccaac gcaactcttc gagaacgata aaacagcgcc caggcaacga ccaggcgtta
     8161 tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gttcacccgc ctgcaccagt
     8221 aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa ccactcatac
     8281 aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg atccattttt
     8341 tgcagcagcg ccagatgctg cgcgatctgc gtcagtgcca gatgaccggg atccagcatc
     8401 gacgccagtt tttccaccaa tgtcagacgc agactaagcg gcgcatttaa catcccgccg
     8461 ttcgtccatg tctgaagctg cacgcgcgca agaagttcct gcatcagtcg ttcacgaaac
     8521 tgctgctgat gggcttgtgg aaggcgggca tcatcgccct gcgccagatc cactaaaaag
     8581 cggggataaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt ggtcaatatc
     8641 gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg gtgtaaatcc
     8701 agccgtgagt catcacagtc tgctgcaatt gctgttgcat cagcctgacc accgattcat
     8761 tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattccgtc aaacatgaag
     8821 cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc tgctctgcca
     8881 gaactttagc catttgttgg caataaagat cgacttctgc gcttaatgct cgttgcaaca
     8941 cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag tcaggctgat
     9001 tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaatcg cgtgctctgc tcgtccgttg
     9061 cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg ggaactacgc
     9121 cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat tccattttga
     9181 cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg gattcaagct
     9241 cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt aacagtgtct
     9301 cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag gcggctaact
     9361 gcaccttcag cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc agggccagct
     9421 cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat agctgcaaac
     9481 tttcttcgat ctggtggata ttttgccgca attgttcaca agcgacgttt aacccgtgcg
     9541 cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta tgcgcggcag
     9601 atcgcagagc atacaacgag gcgttagcgt aagcggcatg aagcaacatc tgaattggct
     9661 gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt tcgaggtccg
     9721 ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct tccacccagc
     9781 gttgttgctc tggcggtggt aacttaccgt tgttggctaa ctcatggcgc gcccgatttg
     9841 ccaggtagcc ccacatcgac gacaccggaa atatctgctg tggcgtaata cagcctttca
     9901 tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta cgatcctgtt
     9961 gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc aaaatcgcct
    10021 cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc gccagtaccg
    10081 ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc ccggcttcat
    10141 ttggcccggg ggtatccagt aacgtcaatt gaccgggata actttccagc cccgccagat
    10201 ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca gaaaaaggaa
    10261 aatcgacgtc cagcgccttc gccagtcgca ccaaatcatt caaacttttc agacaatgaa
    10321 aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg atccgctgca
    10381 taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta atatcgcaat
    10441 cacgcaggcg ctgttgtaat ttttgaatta aacaatcgat tggcgcgaca tgtgaaaaat
    10501 gcagtaccgg ttccttttgg ccgggcgtat ggcgaataag cgtcggcagc gcagtcattg
    10561 ggcgattgcg attaggcagt acctccgtac caacaatggc attaatggtg gttgatttcc
    10621 ctgctttcat ggtaccgaca atcgccagca ccatttccag tcgggaaatt ttacgcaact
    10681 cattattcag catcgcgtga cgctcggcga tattaggctg actccagggt aaagccagtt
    10741 gtggcgcatc gtctccgggt acagagagag gcattttttc cagtaactgc aactgttggc
    10801 gagaaagctg taacagccgt tcagcctcct gacttaactc atacagggtc tgtgtgtaca
    10861 tagaaaattc ttccttaaag caaattttat tacttttatt tagccagat
//
LOCUS       DAZFDC010000143        10834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFDC010000143 DAZFDC010000000
VERSION     DAZFDC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.143"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..351)
                     /locus_tag="ACHJ48_004021"
     CDS             complement(<1..351)
                     /locus_tag="ACHJ48_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709472.1"
                     /translation="MKKAAILIDAGFFMQRVHATHRKHFAEHELTAQCIMKVIWSMVL
                     SHLNGKRQSQERREPLELYRIYFYDCPPLDIQTRLPLPEPGNKTPGRKNFKLEKSYIL
                     RTELHEELRKTRKTA"
     gene            complement(479..1192)
                     /locus_tag="ACHJ48_004022"
     CDS             complement(479..1192)
                     /locus_tag="ACHJ48_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4709473.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            1293..1493
                     /locus_tag="ACHJ48_004023"
     CDS             1293..1493
                     /locus_tag="ACHJ48_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4709474.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            1612..1905
                     /locus_tag="ACHJ48_004024"
     CDS             1612..1905
                     /locus_tag="ACHJ48_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO4709475.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGGVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            1938..2860
                     /locus_tag="ACHJ48_004025"
                     /pseudo
     CDS             1938..2860
                     /locus_tag="ACHJ48_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            2857..2919
                     /locus_tag="ACHJ48_004026"
                     /pseudo
     CDS             2857..2919
                     /locus_tag="ACHJ48_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
     gene            2920..3168
                     /locus_tag="ACHJ48_004027"
                     /pseudo
     CDS             2920..3168
                     /locus_tag="ACHJ48_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
     gene            3168..3962
                     /locus_tag="ACHJ48_004028"
     CDS             3168..3962
                     /locus_tag="ACHJ48_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4709476.1"
                     /translation="MAKNDFKAFATDRNANVMSQEEWEALPALISGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALQNLGLGEGAPAIGVPFFWPSAAMPNTVIDSWSCMVFLKFNGAKFSATDYP
                     VLAKVFPSLVLPEARGDFIRIWDDGRGADGGRELLSWQAATNFSQFAGNIGEGAGHAI
                     NFHDGIAGNQPGFSRFNFTSNSVGDGVNFVAVRPRNIAFNFLVRAK"
     gene            3962..4555
                     /locus_tag="ACHJ48_004029"
     CDS             3962..4555
                     /locus_tag="ACHJ48_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4709477.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGRVAVFTGKGWSHEEDHRNETVYSIENGAAVTVDYIGAIKDGYVT
                     ISPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(4527..4970)
                     /locus_tag="ACHJ48_004030"
     CDS             complement(4527..4970)
                     /locus_tag="ACHJ48_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFO4709478.1"
                     /translation="MDEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWADIPPPTHEELIEITESERQLLINQANEYMNSKQWPGKA
                     AIGRLKGDELTQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
     gene            complement(4991..5401)
                     /locus_tag="ACHJ48_004031"
     CDS             complement(4991..5401)
                     /locus_tag="ACHJ48_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4709479.1"
                     /translation="MPFARYFCIFINVGLGEAAKRDVGTGENQIPDMASFASGNGWMK
                     LPNGKILQYGRGAVTPTLSTQTMRITFSIPFPKKADCAMLTHSGDGGAPLGAGRGFVM
                     TAEGPTLTGFNSAYRTSSTSDTVSMNYSWWAVGE"
     gene            5431..5985
                     /gene="pinE"
                     /locus_tag="ACHJ48_004032"
     CDS             5431..5985
                     /gene="pinE"
                     /locus_tag="ACHJ48_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HFO4709480.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIAAGIP
                     RQKVAIIYDVGVSTLYKKFPAGDK"
     gene            complement(6043..6816)
                     /locus_tag="ACHJ48_004033"
     CDS             complement(6043..6816)
                     /locus_tag="ACHJ48_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709481.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAAADDDDLLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETITFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASKAEVILATVRGIVRGVFIADKWLKSTREHFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            7640..8383
                     /locus_tag="ACHJ48_004034"
     CDS             7640..8383
                     /locus_tag="ACHJ48_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4709482.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLRDISDCREYPSYLKINFADFSLIKGLISWANHCAE
                     YIEIFDESIAFTCLSAFSSEKQFGVFLFGCLKSTGAKVKTIIHTDLSAPWRLKDISSR
                     LYLSESLLKRKLKEEGVSFSKIILDERMQMAEYLLSTRCYPISKVAKVCGYASVSYFT
                     YVFRRYFGVSPSQYSQRSSESKILTHQGI"
     gene            complement(8425..8859)
                     /locus_tag="ACHJ48_004035"
                     /pseudo
     CDS             complement(8425..8859)
                     /locus_tag="ACHJ48_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            9346..10527
                     /locus_tag="ACHJ48_004036"
     CDS             9346..10527
                     /locus_tag="ACHJ48_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4709483.1"
                     /translation="MPLNDMQIRRAKPEAKAYTFGDGLGLSLLIEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLAMQTESENAFEK
                     IAREWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVFRYAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPDFLRA
                     LEGYSGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVP
                     LSSQAVDLLNELKIMTGNYRYVFPGRNDPNRPMSEASINQAIKRIGYGGKVTGHGFRH
                     TLSTILHEQGFESAWIEIQLAHVDKNSIRGTYNHAQYFSGRKSMMDWYSNLIFERLKR
                     S"
     gene            10531..>10834
                     /locus_tag="ACHJ48_004037"
     CDS             10531..>10834
                     /locus_tag="ACHJ48_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709484.1"
                     /translation="MSQPLSEILTWDDEQWEVFVHDWLIVCKSDDYPWSERLGGAGDK
                     GRDVVGYKSDPNVEGYSWDNYQCKLYKKSLGFSDVVVEFGKLIYFTLNGDYPIPQKY"
BASE COUNT         2947 a         2300 c         2547 g         3040 t
ORIGIN      
        1 ggctgttttt cgagtttttc ttaactcttc atgcagctcc gttctcagaa tatatgattt
       61 ttcgagtttg aaattcttgc gaccaggcgt cttattgcca ggctcaggaa gtggaaggcg
      121 tgtttgaatg tcgagtggtg gacagtcata gaagtaaatt ctataaagct caagcggttc
      181 cctacgttct tgtgattgac gttttccatt aagatgggaa agaaccattg accatattac
      241 tttcattatg cattgcgcag tcagttcatg ctcggcgaag tgtttacgat gcgtagcatg
      301 aacacgctgc atgaaaaaac ccgcatcaat taaaattgct gctttcttca taagagtccc
      361 aaaaaaatag cccagagccg ttatgcagat attaacaatt gtctgcgaac ggggctgggc
      421 ttggtttaat taatctatgc caagttgact gtgccgtcaa cataaaactt acttcctatc
      481 acccaaacgt ctcttcaggc cactgactag cgataacttt ccccacaacg gaacaactct
      541 cattgcatgg gatcattggg tattgtgggt ttagtggctg tagaaacacc tgaccgctat
      601 ccctgatcag tttcttgaag gtaaactcat caccaccaag tctggctata cagaaatcgc
      661 ctggctcaac agcctgctca gggtcaacca gaattaacat cccgtcagga aagctgggct
      721 tggatcctgt tggtgcggtc atggaattac cttcaacctc aagccagaat gcagagccac
      781 tggctttttt ggttgtgctt acccatttct ccgcatcgcc tttggtaaag gttctaagct
      841 caggcgagaa catcccggcc tgaacatgag aaaaaacagg gtactcatac tcacttctaa
      901 gtgacggctg catactaacc gcttcataca tctcgtagat ttctctggcg attgaagggc
      961 taaattcttc aacgctaacg ttgagaattt ttgcaagcaa tgcggcgtta taagcattta
     1021 atgcattgat gccattaaat aaagcaccaa cgcctgactg ccccatcccc atcttgtctg
     1081 cgacagattc ctgggataag ccaagttcat ttttcttttt ttcataaata gctttaaggc
     1141 gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt ttttgcgctc atacgttaaa
     1201 tctatcaccg caagggataa atatctaaca ccgcgcgtgt tgactacttt acctctggcg
     1261 gtgataatgg ttgcatgtac taaggaggtt gtatggaaca acgcataacc ctgaaagatt
     1321 atgcaatgcg ctttgggcaa accaagacag ctaaagatct cggcgtatat caaagcgcga
     1381 tcaacaaggc cattcatgca ggccgaaaga tttttttaac tataaacgct gatggaagcg
     1441 tttatgcgga agaggtaaag cccttcccga gtaacaaaaa aacaacagca taaataaccc
     1501 cgctcttaca cattccagcc ctgaaaaagg gcatccaatt aaaccacacc tatggtgtat
     1561 gcatttattt gcatacattc aatcaattgt tatctaagga aatacttaca tatggttcgt
     1621 gcaaacaaac gcaacgaggc tctacgaatc gagagtgcgt tgcttaacaa aatcgcaatg
     1681 cttggaactg agaagacagc ggaagctgtg ggagttgata agtcgcagat cagcaggtgg
     1741 aagagagact ggattccaaa gttctcaatg ctgcttgctg ttcttgaatg ggggggcgtt
     1801 gacgacgaca tggctcgatt ggcgcgacaa gttgctgcga ttctcaccaa taaaaaacgc
     1861 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1921 tatcaacagg agtcattatg acaaatacag caaaaatact caacttcggc agaggtaact
     1981 ttgccggaca ggagcgtaat gtggcagatc tcgatgatgg ttacgccaga ctatcaaata
     2041 tgctgcttga ggcttattcg ggcgcagatc tgaccaagcg acagtttaaa gtgctgcttg
     2101 ccattctgcg taaaacctat gggtggaata aaccaatgga cagaatcacc gattctcaac
     2161 ttagcgagat tacaaagtta cctgtcaaac ggtgcaatga agccaagtta gaactcgtca
     2221 gaatgaatat tatcaagcag caaggcggca tgtttggacc aaataaaaac atctcagaat
     2281 ggtgtatccc tcaaaacgag ggaaaatccc ctaaaacgag ggataaaaca tccctcaaat
     2341 tgggggattg ctatccctca aattggggga ttgctatccc tcaaaacagg gggacacaaa
     2401 agacactatt acaaaagaaa aaagaaaaga ttattcgtcc gagaattctg gcgaatcctc
     2461 tgaccagcca gaaaacgatc tttctgtggt taaaccggat gctgcaattc agagcggcag
     2521 caagtgggga acagcagaag acctgaccgc cgcagagtgg atgtttgaca tggtgaagac
     2581 catcgcgcca tcagccagaa aaccgaattt tgctgggtgg gctaacgata tccgcctgat
     2641 gcgtgaacgt gacggacgta accaccgcga tatgtgtgtg cttttccgct gggcctgcca
     2701 ggacaacttc tggtccggta acgtgctgag tccggccaaa ctccgcgaca agtggaccca
     2761 gctcgaaatc aaccgtaaca agcaacaggc aggcgtgaca gccagcaaac caaaactcga
     2821 cctgacaaac actgactgga tttacggggt ggatttatga aaaacatcgc cgcacagatg
     2881 gttaactttg accgtgagca gatgcgtcgg atcgccaaca tcgataacca ggacatgacg
     2941 atatcggtct gggtctttcc tgatactgat atttcagatg tatcgcgtga gttaattgcg
     3001 gcaattaaac aggggtatct aacagtaaaa gccgccgggg tatgggcggg gggcattgaa
     3061 acaccttcgg tggaaactcc atcggaaggc tctaaatttt ttggttttga tatggataac
     3121 gaattcatca gtggttttga tgtaggggca tggggagtat tactctgatg gcgaaaaatg
     3181 actttaaagc gtttgcaact gatcgaaatg ccaatgttat gtcgcaggag gaatgggaag
     3241 cgttgcctgc gcttatatcc ggatttacag cagggaaagc atccagtgcg caagtcaata
     3301 aggttattcg gcaggccagc tttattgctg cagctctggc ccagtttgta agtgataaaa
     3361 cgcaacggga tgtgcttgat aatggtgatc tgcccggttt tgttgaattg ctgggatcgg
     3421 ggtttgctgt tgaatacctg agccgcaaga atccgtttgg cgatatcaaa tcggacggca
     3481 cggtgaaaac ggctcttcaa aaccttggtt tgggagaagg tgctccagct attggcgttc
     3541 cgttcttctg gccgtccgct gcaatgccaa atactgtaat cgacagctgg tcctgtatgg
     3601 tgtttttgaa gttcaacggc gcgaaattct ctgccactga ttatcctgtg ctggcgaaag
     3661 tgtttccttc gctggtatta cctgaagccc gcggtgattt cattcgtatc tgggatgacg
     3721 ggcgaggtgc agacggtggt cgcgaattat taagctggca ggcagctaca aacttttctc
     3781 agtttgccgg gaatataggc gaaggtgcgg gacacgcaat taactttcat gatggcatcg
     3841 ccggaaatca gccaggattt tcacgattta atttcaccag taactctgtg ggtgatggtg
     3901 tgaattttgt tgcagtcaga ccgcgaaata ttgcatttaa ctttctggtg agggctaaat
     3961 aatgaaacct gtttttgatg aaaatgggct ggctacagtg ccgggcgata tgcgttgttt
     4021 ttattatgat gctgaaacat ctgagtatac gggctggtct gatgaatata ttaatactgg
     4081 cgtaagtatg cccgcctgtt ccactggtat tgaccctggc gaaaacattc cgggaagagt
     4141 ggcagtattt acaggtaagg gatggagcca tgaagaagac catcgcaatg agactgttta
     4201 ctcaatcgaa aatggcgcag ctgttacagt ggattatatc ggtgccatca aagacggtta
     4261 tgtcacgatt tcaccgttaa cgccatacga taaatgggat ggtgagaaat gggtgacaga
     4321 cactgaggca caacacagtg ccgcagtaga cgcggcagaa gcacagcgcc aatcactgat
     4381 tgatgcagca atggcttcca ttagtctgat tcagctgaag ttacaggccg gacggaaact
     4441 gactcaggca gaaacaaccc gacttaacgc cgtgctggat tacattgacg cggtgacggc
     4501 aacagatacc agcaccgcgc cggatgtcat ctggcctgaa ctgccggagg cgtaggccat
     4561 tcaatatctg gcgcaccgga agtatcgacc agctccagtg cgtccagata atccagccac
     4621 aaattatatt gcgtcagttc gtcacctttc agacgaccaa tagccgcttt accgggccat
     4681 tgcttactgt tcatgtattc gttggcctgg ttaattagta gctgtctttc tgattcagta
     4741 atttcaataa gctcttcatg cgtgggtgga ggaatatctg cccacgcagg cagcccatca
     4801 tctccggcaa tacggatttt tccttgtggc ggttcagcca taaactcact gataatattt
     4861 tgatttactt ccttagcgtc tgataaatcc catccctctg atttatattt atcaatcata
     4921 tccacaggga aaaaagcatt atgccttgcg ctataaacat attcgtccat ataaatcacc
     4981 ctgaataaaa ttactcacca acagcccacc aactgtaatt catcgatacc gtgtcgctgg
     5041 ttgatgacgt tctgtaagca gaattaaagc cggttaacgt tgggccttct gcagtcatca
     5101 cgaaccctcg cccagcgcct aaaggcgcac cgccatcacc agaatgagta agcatggcgc
     5161 agtccgcttt tttggggaaa gggatgctga atgtaattct cattgtttgc gtcgataatg
     5221 tcggcgtaac cgcaccacga ccatattgca ggattttccc gttgggtaat ttcatccatc
     5281 cattaccact ggcaaaagag gccatgtccg gtatctgatt ttcccctgtc cccacatccc
     5341 gttttgccgc ttctcccaaa ccaacgttta tgaaaatgca gaaataacga gcaaatggca
     5401 tcattcctgc ttttgtcagg gagatctacc atgcttattg gctatgtacg tgtgtcaaca
     5461 aatgaccaga acacagatct acaacgtaat gcgctgaact gtgcaggatg cgagctgatt
     5521 tttgaagaca agataagcgg tacaaagtcc gaaaggccgg gactgaaaaa actgctcagg
     5581 acattatcgg caggtgacac tctggttgtc tggaaactgg atcggctggg gcgcagtatg
     5641 cggcatctgg tcattctggt tgaggagttg cgcgaacgtg gcgttaattt tcgcagcctg
     5701 acggatgcta ttgataccag taccccaatg gggcgctttt tctttcatgt gatgggggcc
     5761 ctggctgaaa tggaacgaga actgattgtt gaacgaacaa aagctggact ggaagctgct
     5821 cgcgcacagg gacgaattgg tggacgtcgt cccaaactta caccagaaca atgggcgcag
     5881 gccggacgat taattgcagc aggaattcct cgccagaagg tagcgatcat ctatgatgtt
     5941 ggtgtatcga cactgtataa gaagtttccg gccggagata aatgaaaccg tagcacgtcg
     6001 tatgcaagat cgtgctgcgg tttatgctta ccacttgaag attcaaaaat taggtgagta
     6061 acgaactgga gacatagctc ctttttttct taattcatct ggtatttttt tcccaagata
     6121 aaggtttgct atttcaggtg gggcttctcg accttcaaaa ccatagcgag agctttgtgt
     6181 tgcctcaaag tcagggtctt cgtcccagta tttcatcgta ggaaaatgtt cacgcgttga
     6241 ttttagccat ttatcagcaa tgaaaactcc tcgaacgatc ccccttacag tagcaagaat
     6301 gacttctgct ttactggcgc gcgagacatt aatgcgccag ctaaatcgaa ccgcatcata
     6361 aagctctgaa tcttttgcac ttctgttaac ggaaatcatt aatgctttat gatgaaatgt
     6421 tatggtttcg ggttgatatg ttgctatcaa ctctttgaca tgcgcggcgc cgaattcatt
     6481 gctgccagca ccattcatga tattcgttaa cccagggtag gcatcaataa gtgctgcttc
     6541 aacttcgtac gccgtctttt catcagtcat tccatgtcga tggatgacat ggataacttc
     6601 aagtcccgct aatcttattt ctctgatttg ctttagcttg ttgctcagta agtcgtcatc
     6661 atcagccgct gccacttcac cgcgcatatg ggcaaatacg cggttacctt tgcctttccc
     6721 tacatagaag gtgcttccgt ccctaggatc aatcaatcgg tatacatacc agccaaggtg
     6781 ttcaattact ccagaaggaa actcagtaat atccattttg caatatctgt gaattatttg
     6841 tgagacgtat attaatgaac attgcaaggg ctcacaacta gtagtgttga gaaagccatc
     6901 gggaaaatga ggctaaccct ttgaatttac atagcgcaaa aagatacctt tcctcataat
     6961 gtgagctaat tttatgtttc gtttgatgat cgggtcggtc tcgaaaaccg gagtaggggc
     7021 aactctaccg ggggttcaaa tccccctctc tccgccactt tatcaatgac ttatctcccg
     7081 acttcccgtc ttgcttttcc taaacagaac aatcgtagaa tattcttgaa gggttagatc
     7141 gtcactgttt tctgttcgat actgtgacat tcagcacttg attcgctatg gatctgacag
     7201 gaaggttttg agcgaaaatc tgcagttatt cagtcgtttt cttatcggtc accattattc
     7261 ttttagacat tgatcctaca aagctgccgc aaagttggtg gtgggaactg aagttgcgta
     7321 gagaaggggt caatacccgg aggcaaacat gggctggcaa aagtgtagcg gtattaggcg
     7381 cagctattta gcctagttat gttttatgaa aacttgatat catataagtg tcttacttat
     7441 tggctgtaaa taagtttttc ctaaggaatt gtttcttgag tatcatttgt aactgtaacg
     7501 gaatttataa tcctttgctt tattgttacg gtatttttta tcacacccta tttttagtgg
     7561 ttttttatac tgaagtttgg caaagtgaac tttatataca tatacttcat cctggtttca
     7621 gttaaattgg ggggatgata tggcaactac atgttcagtt atattgattt tggagtcctt
     7681 tgatgtttat ttcggaaaag agagtgtgtt tctggagaga ggttcatctg tacttgtcga
     7741 ctctagctct agagattttt tcctgacata tcctgaaaga gtgatagtgg cggattttgg
     7801 cgctgagttt attagtcgct atttgaaagc taataactta agggatattt ctgattgtag
     7861 ggaatatcca tcttatttaa aaataaactt tgctgacttc agtttaatta aaggattaat
     7921 tagttgggct aatcactgtg ctgaatacat agaaattttt gatgagtcta ttgcttttac
     7981 atgtctctct gcattttctt ctgaaaaaca atttggagta tttctgtttg gatgtttgaa
     8041 aagcacaggg gctaaagtta aaacgattat tcatacggat ttatctgcac catggcgtct
     8101 taaggatata tcatcaagat tatatctcag cgaaagttta ctaaagagga aattgaaaga
     8161 agagggggta tcattcagta agatcatact tgatgagagg atgcaaatgg ctgaatattt
     8221 actcagcact cgttgttatc ctattagtaa agtagctaag gtctgtggtt atgccagtgt
     8281 ctcatacttt acttatgtat ttagacgtta ttttggtgtt tctccaagtc aatactctca
     8341 gaggagttca gaaagtaaaa ttcttactca ccagggaatc tgatcattgt tcttgccccc
     8401 ttatttccag acaggggggg tatcttaagt taacgttacc cgctgacgtc gatattctcg
     8461 cggagagcga taacccaacg cactgtgcgg atggttttca ttgtaatgtt cgatcgccac
     8521 tgcaagatta tgcaatgccg ttcttacatt cggtttcggc atgaacgcga tgtagtcttc
     8581 cttcatcgtt ttcacgaacc tttctgcaat tccattactc tgaggactgc tgattgccgt
     8641 tgtgcagggc tccagattca actctctggc gaactgccgt gtttcatgcg ctctatacgc
     8701 tgaaccgtta tctgtcagcc actggatgga ctgttccggt actttatcgc caaagcgttt
     8761 ttctatcgct cctaacatga catcctgcac tgtcgcttta tcgtaacctc ccgtgcttgc
     8821 ggcccagtct atggcttcac gatcgcaaca gtccagggcg attcatctgg gccaatcctt
     8881 gctcataaca ggcattcact tcagtcatgg cagaaaggta tgcatgctgg agaagtcgtg
     8941 aaagaaaaga agactgctgc gccgtttgtc gtcacgttta tcttcattgg ctatgcaagt
     9001 cgtaatacaa ggtgggacaa aactgagaca cataaggcct cgcaatggct tgcaaggctt
     9061 tacatgtttt gatgtggtgg gacgtgtgag cgcagtgttg atggggtaat gctttgaatt
     9121 agaagcggat tcttataatt cgtaatgcga aggtcgtagg ttcgactcct attatcggca
     9181 ccatctcaac ttcctcaaac gtccgtatta gtccataaaa tctctgattt ataacatttt
     9241 ttattctttt aagtccatag tagtccgtaa ccatccagta gaatccggta ctgaatgtgt
     9301 ataggattgt gtatatgttc ctgttcggtc tggattccta tacacatgcc tttaaacgat
     9361 atgcagattc gccgcgctaa gcctgaagct aaagcctata catttggaga tgggctaggg
     9421 ttgtcattac ttatagaacc taatggaagc aagagttggc ggttccgcta tcgctatgcc
     9481 ggcaaaccca aaatgatctc gcttggtgtt tacccaacga tcacccttgc cgatgctcgt
     9541 tcccgtcgtg atgaagctcg aaaacttgtg gcagaaggaa agaaccctag tgaggttcga
     9601 aaagagcaaa agctagctat gcaaacagag tcagagaacg ccttcgaaaa gatagccaga
     9661 gagtggcatc aacttaaatc tgctaaatgg tcggcgggat atgcatcaga catcatggaa
     9721 gcgtttaaga acgacatttt tccttatgtc ggaacaaggc ctgtgggaga gattaaaccg
     9781 ctagagctgc tgaacgttct gcgtaaaatt gagaaacgtg gtgcgttgga gaaaatgcgc
     9841 aaagtgcggc agcgttgctc cgaagtgttt cgctacgcaa ttgcaacggg tagggcggag
     9901 tacaatcctg cggctgatct ctccagcgct ctcgaagtac accaatccaa tcatttccca
     9961 ttcctaaaag ctgatgagat acctgatttt ctacgtgcct tagagggtta ctccgggagt
    10021 aagcttgtcc agatagccac gaaattactg atgattacgg gtgtgagaac catcgaatta
    10081 cgcgcggcat tatggcaaga atttgatctg gataacgcta tttgggaaat tcctgctgaa
    10141 aggatgaaaa tgcgtaggcc acatcttgtg cccttatcat ctcaagcggt agatttactc
    10201 aatgaactca agatcatgac agggaactat cgttatgttt ttccagggcg gaacgatccg
    10261 aataggccaa tgagcgaagc gagtataaat caagccatta agcgtattgg gtatggagga
    10321 aaagtcactg gacatggttt tcgtcatacc ctttctacaa tcctgcatga gcaaggtttt
    10381 gagagtgctt ggattgaaat ccagttggct catgtagata aaaattctat tagggggact
    10441 tataaccatg ctcaatattt tagtggaagg aagtctatga tggactggta cagtaatttg
    10501 atatttgaaa gactaaaaag gagttaattt gtgtctcaac cactttcgga aatattgaca
    10561 tgggatgatg aacaatggga ggtatttgtc catgattggc ttattgtctg taaatcagat
    10621 gattacccgt ggagcgaacg tttgggagga gctggagata aaggtagaga cgttgttgga
    10681 tataaatcgg atcctaacgt agaaggttat tcttgggata attatcaatg caaactgtac
    10741 aaaaaaagtt tagggttctc tgatgttgta gttgagtttg gaaaacttat ctattttact
    10801 ctgaatggtg attatcccat ccctcagaag tact
//
LOCUS       DAZFDC010000144        10370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFDC010000144 DAZFDC010000000
VERSION     DAZFDC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.144"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            213..1106
                     /locus_tag="ACHJ48_004038"
     CDS             213..1106
                     /locus_tag="ACHJ48_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709485.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQSLLNDFSHRLKMEG
                     EEQLFHCVNELKYGLSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVAPDILV
                     LPVNVYHADFKIKINNAVEKT"
     gene            1113..2027
                     /locus_tag="ACHJ48_004039"
     CDS             1113..2027
                     /locus_tag="ACHJ48_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4709486.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEQPLFVKHTAMMREACKELPQLVEYVPLACEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKKRMAELFNQSDTSVRNKYRPAFDKWNKLV
                     DKLNHERNTVPFACLTTIIATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2721..2939
                     /locus_tag="ACHJ48_004040"
     CDS             2721..2939
                     /locus_tag="ACHJ48_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO4709487.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            3156..3398
                     /locus_tag="ACHJ48_004041"
     CDS             3156..3398
                     /locus_tag="ACHJ48_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO4709488.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3728..4657)
                     /gene="fdhE"
                     /locus_tag="ACHJ48_004042"
     CDS             complement(3728..4657)
                     /gene="fdhE"
                     /locus_tag="ACHJ48_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO4709489.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4654..5289)
                     /gene="fdoI"
                     /locus_tag="ACHJ48_004043"
     CDS             complement(4654..5289)
                     /gene="fdoI"
                     /locus_tag="ACHJ48_004043"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO4709490.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5286..6188)
                     /gene="fdoH"
                     /locus_tag="ACHJ48_004044"
     CDS             complement(5286..6188)
                     /gene="fdoH"
                     /locus_tag="ACHJ48_004044"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO4709491.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNHADEEENNLHEEKDEERK"
     gene            complement(6201..9251)
                     /gene="fdnG"
                     /locus_tag="ACHJ48_004045"
     CDS             complement(6201..9251)
                     /gene="fdnG"
                     /locus_tag="ACHJ48_004045"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8664..8666),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO4709492.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQENYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVISCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9445..10278
                     /gene="fdhD"
                     /locus_tag="ACHJ48_004046"
     CDS             9445..10278
                     /gene="fdhD"
                     /locus_tag="ACHJ48_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO4709493.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2641 a         2466 c         2621 g         2642 t
ORIGIN      
        1 catcatctta gttcaggttt gagcgatttt tgatagtcaa aaccccctct ggtgccatag
       61 ttttctcata aatactttaa aaatctatag ctaacaaaca ttatgcaaag ataatgggct
      121 ggcatcattg tcagtatcat tcatttactg ctctggatgt tataaataat tatgtagcat
      181 gtttgctctt aaaccgtggg aaggaggctg taatggttgc caatattaat ctcattaaac
      241 aagaaagtta ttctgttgtt aatttagaaa aacaactaat gagtaatgaa tcaccgaagc
      301 aatcgctatt aaatgatttt tcacatcgcc ttaaaatgga aggtgaggaa caactgttcc
      361 attgtgttaa cgaactgaag tatggtttgt cgagtaataa tgaatatgac ctgtttaaaa
      421 atagagaaac ctcactgtgg aaacgcaaag taccagaaag cgcagcgctg gcaatgacgg
      481 gaacagtata taaattattt ggtagtcgct tactcaacgc ccgcaatcag ttagataaaa
      541 atctcctcga aatggctatg caaatggttt atggcaaaga tatggtgaca aagtccgggg
      601 atatattgat tgatatacaa ttaaataagg atggcagcct ccagagtaag cactacacat
      661 ttgatgtagg tgaagttata aacagttata atattgacct ctttgtgaat gcttcgagta
      721 ttaatcaaac ttacttacat gataaaaatc ctggtgatgt tattagcctg tatggcactt
      781 tagataacat tcctcttgta gttaaggaat gcacgggtaa aaatcatcaa attaacgtta
      841 aagccaaatt gccacaagaa cgaagtgaaa aagttgagga tatgtgtgaa agaatgagag
      901 gtggcatctc tatgtttaat cacaccacaa aaacagctgg taatatagag cataatttgc
      961 agctaagttt tctcgtggat tcacggccgt cgatcaccaa taaatactca gccaaagatg
     1021 tagcgccaga catacttgta ttgcccgtca acgtatatca tgcggatttt aaaattaaaa
     1081 tcaataatgc agtagagaag acttagtcaa ttgtgttatg tgctgtgcaa aaaaatctcc
     1141 atgcagcaag gaaggttctt agcgggataa taggtaatgt accaaaaaat aataaaccaa
     1201 aaacaatcat ttttgattta cgttttctta gtgcaattaa gcagaaagtt tttttgggca
     1261 gtgaacaacc gttgtttgta aagcataccg caatgatgag ggaggcatgt aaggaattac
     1321 cgcagctagt ggagtatgtt cctttagcct gtgaagcgca tagccatcgc agcgtcgccc
     1381 ttgccaaaac cgtggctgca cccatcatgg cgacaggtgt gggcgcggct ttaatgtatg
     1441 caacatcatc aggaccgtat tatgcgcaaa gtctttctgg ttcgccggat atcgagatga
     1501 taaaaaaacg catggctgaa ttgtttaacc agtcggatac ttctgtgcgc aacaaatatc
     1561 gcccggcttt tgataaatgg aataaactgg tagataaatt aaatcacgaa agaaatacag
     1621 ttccgtttgc gtgtttgacc accattatag cgacagcgat aaatgaaact cctgaggggg
     1681 atacgaatgt tgccgttgtg atggggtgta aatctgcgaa agatcgcacg atatccattg
     1741 tccttggcaa ctcgatgttg caaacactct ttgaaaaacg cctcgccgat gatggagaaa
     1801 tagaaaacct ttttgatcaa cagggatatt ttagttgcga ttcattaaca gccgaagaag
     1861 taatgatgtt aaaagacctt tttgatattc gagttcttca tctctctaac aaatttaatg
     1921 ttgggttgca aggtaatatc aacacggatg ttttacaaga ttcgtttttt aaaaatgtcg
     1981 atttcattca tgaatctaac tcgtataccg tagggatggg agtctgaaga gtaaaactca
     2041 ttaggattat cagaattcgg ccaatgcatt gcaaaaatga aatacaaaga gagatgtgtg
     2101 caggcgctta gccgcgaggg gcatggcgaa gccatacaga aagcaccggc attcagaact
     2161 cctgtgaaag cggcatagtg ccgctgctca ggagttttgt tatgacccgt atcgtgctga
     2221 acgttgccac cctatgatgc gattaaaaaa actcaccgtc gagccgatga ttgacgtcaa
     2281 tgctatcgga tgatgccggg ggtgttccgg aaggaattcc ttgtttcccg aactgttacc
     2341 aacaaaatta taacgatgag caagtaaaga ccggttggtg gcagtactga ccggtctgtg
     2401 atggaagatt aatttcacct taaatcctgg tgctgttagg atgctgacgt ttttcgggta
     2461 caccattcag attatgcaaa taaagattca atgaaaaaat aaatatccct gaatataata
     2521 tattcgtagc acccataact catcctgggc gataactaaa ccatttttga gtaacaaatg
     2581 aaacaactgc aacgctctta ttatttctgg aaaacgtctc gcaaaaacaa aacaacatgc
     2641 aaaaatccgc tttcgttaat taatttttcg gcgtaatttt cgcacataca ttaatgagta
     2701 atatatgtaa ggtggtggta atggcgatga atacggtttt tcttcattta tcagaagagg
     2761 caattaaacg gctgaacaag cttcggggct ggcgtaaggt ttcacgatct gcaattttac
     2821 gcgaagcggt agagcaatat ctggaacgtc agcaattccc ggtgcgtaaa gcaaagggag
     2881 gcaggcaaaa gggggaggta gttggtgttg atgatcagtg taaggagcat aaggaatgac
     2941 aaaaattttt cattcttgaa tataaaaaac agatgccctt attctggtat taatagaagg
     3001 ctgttttact tgaacttata ataactgcaa ctgttacatc atatctggaa aacgcctcgc
     3061 aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga ttcgacaagg gtacttttct
     3121 ctccatagat atcaaatgat attggtcaaa tatttatgaa ctctctggca ggtattgata
     3181 tgggcagaat tatactcgat ttatcgaatg aggtgattaa gcaacttgat gatcttgagg
     3241 tgcagcgtaa tcttcctcgt acggagctat tacgggaagc ggtagatcaa tacttggtaa
     3301 atcaatcgca aacagtaaga accagtgctt ttggcatatg gcaagggtgt gaggaagatg
     3361 gtgtcgaata ccagcgtaag ctgcgcgagg aatggtaatg ggaaaaaatg atatcaatca
     3421 gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc gtgaacgcct tacccggcct
     3481 acaaagtcgt ataaattcaa tgtattgcag tttattggta ggcctaataa gcgcggcgca
     3541 tcaggcaatc ttacgcttat catcagtctc atacccataa ccttattgat gtgggggtgg
     3601 gtaaattgag gcaaaacagg cactggcggc atagcttgag gtggtaacgc ggaatacgag
     3661 agatggtgtc gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg
     3721 tcagagatta ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc
     3781 cttcttgctc cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa
     3841 ctttcggttc tttttcctga tagagaattt tcaggtaagt gccgcagtca tcgcagcttt
     3901 cggctttaat cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct
     3961 cgcagttgct gcattttacg cgcacaacgt gccattcagt ttcacacagg ttgcagtgca
     4021 ggtaacgtag cccctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac
     4081 aaaccgggca atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat
     4141 tggccatctg cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc
     4201 tgacggacga gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg
     4261 atgccttctc cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct
     4321 cagcaatcag cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat
     4381 ccagcggggg cttgccttgt gcgctggctt ctttaatgcg cgcagtcaga tccatctcca
     4441 gcggatggtc gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt
     4501 aatcacccag cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata
     4561 aattcttgag ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg
     4621 aacccagctc atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt
     4681 gcggacctca cggtaccagc gcgggtgatg tttcttcgcc caagcgctgg taacccatcc
     4741 ttccaccatg gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat
     4801 aaccacaatt aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg
     4861 gattgagaaa gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag
     4921 aaccaggaaa ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt
     4981 gtcacctact tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc
     5041 ccgattgatt aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat
     5101 aaccacgccg acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat
     5161 caaccagttg aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca
     5221 gaaggcggtg atccagtggt tgatacgttc cggcgcggtg tagcgcacga tggtgtcacg
     5281 tcgtttcatt tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca
     5341 tggttcggac cgacacccac gtagtggaag atactggctg cgaaggtagc cgcaaagcca
     5401 acagctgcga gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg
     5461 ttctccggca agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacataacg
     5521 tgtgtaccac cgacgcctgc cggatcgtac aggcccgcat tgtcgtaacc gcgggttttc
     5581 agctccgcca cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc
     5641 gcgcccgttg ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg
     5701 cacagcgtac atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga
     5761 cagcccgcaa tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg
     5821 ttggcatact gaataattgc accttccgcc gggcacgctt tcagacagcc tggatcggaa
     5881 cagtgcatac agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc
     5941 gagaagcgca tcaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca
     6001 atgttattgc cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct
     6061 ttacagccga tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc
     6121 gcctgaggcg cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga
     6181 taagccatat gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc
     6241 ggcgtctgcg tgttcgcatc accgacgaat ggcgtcaacg tattggcaat aaagcctttt
     6301 ttcgcaacac cttcatagcc ccagtgaata ggaataccga tggtatcgat atctttgccg
     6361 ttcgctttca gcgtgcgaat acgtttggtc accaccgctt tggctttgat atagccacgg
     6421 ttggaggaga ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc
     6481 ccgatttcca caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag
     6541 tagtggaagt gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta
     6601 cccaatgctt cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga
     6661 tgtagcgggt tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct
     6721 tccgccatct tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc
     6781 ccgacgccgc tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta
     6841 gtgccgtccc atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg
     6901 gaggcgcggt tatacagaat gcggcggtta agcggccatg cccatgccca acccagcgtg
     6961 ttaccgaggc cagacggatc ggcgttatca cggcgtgcca tctggttgcc ttccggcgtc
     7021 cagctaccgg cgaaaatcca gcaaccacag gaagttgtac cgtcatcgcg cagttgggca
     7081 aacgagctaa gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata
     7141 tcggccagcg ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga
     7201 atggcgtagt tccaggtcat gttcagcacc tggtccgggt tcgcgccacc ctgttcggca
     7261 tacatcttgc gcaagcgcag gaagataccg gagaggatct cgccatcagt cagcgcaatc
     7321 cccggggcgt ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa
     7381 ccgttctctt ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac
     7441 gagtcaactt cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc
     7501 gggtcgatgg tgacgaggaa cttcagtttc gacagacaac tgatcacttt gtttttgttc
     7561 gggaatgagg caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc
     7621 atcatctcga agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag
     7681 ccccagctat tttctgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag
     7741 aatttcgggt agttgcccca gtagttttcc tggccttcca gcagcggttt tggcgtgttg
     7801 gcggtaaggt aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc
     7861 aggctctgcg acagcagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg
     7921 gcgttaacgc cgccgccagc catccccatg ttaccgagca gcagctggat catcgccatc
     7981 gtacgaatgt tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag
     8041 gcagttttat cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct
     8101 tttggcgtac cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc
     8161 agcaagttcc acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt
     8221 tcgtccagtt cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg
     8281 gtgaacaggc catcttcaaa gccgtaatcc tcacgcacga tcaggctggc gttggtatag
     8341 gcttcagtgt attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac
     8401 aggaaagcaa tgtcagtacc ggaacgaata ggggcatagt agtcagccac cgcagccgta
     8461 cgcgtaaagc gaggatcgat cacaatcagc ttcgcgccat tgtgaatttt ggcttccatc
     8521 gcccagcgga acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg
     8581 ttggcgttct tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca
     8641 agacttgcta ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc
     8701 agcgcgcggg agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac
     8761 agcatcccgg tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag
     8821 ttagcatcgc ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg
     8881 atttgctgcc atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg
     8941 gagtggatga aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg
     9001 tgatccggat cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg
     9061 aggctgtaca tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg
     9121 cgggtgcgca gcagtttata ctgccgggtt tccgcgagtg ctacgctggg tgcaaaaccc
     9181 agtgccgctg ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg
     9241 ctgacctgca tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc
     9301 tgcgtgtgca gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat
     9361 tggctcaatt tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt
     9421 gtgttcttat ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg
     9481 acaaatatca caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca
     9541 cggttagatg aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat
     9601 gtggtgatga tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc
     9661 gaggggataa tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat
     9721 ggtcttgaag tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc
     9781 cgggcgctgg cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc
     9841 ggaaaaccgg tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat
     9901 gcattacgtc atctcaacga ttttcagcca gtgggacaac tgaccggttg tactcacgcc
     9961 gccgcctgga tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat
    10021 gtggcgctgg acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt
    10081 gcggtactgg tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc
    10141 gtagagattt tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgc
    10201 tgtaatctga cactggtagg tttttgtaaa ccgggtcggg caacggttta tacccatccg
    10261 cagcgtttga gcaattaatt aaaataagtc ctgcgatata tattaaattg caggacttat
    10321 tcatttcgtg aattttatta ttttatttat aaacaaaatg cgtgtagtgg
//
LOCUS       DAZFDC010000145        10370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFDC010000145 DAZFDC010000000
VERSION     DAZFDC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.145"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            231..806
                     /gene="ssuE"
                     /locus_tag="ACHJ48_004047"
     CDS             231..806
                     /gene="ssuE"
                     /locus_tag="ACHJ48_004047"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO4709494.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            799..1758
                     /gene="ssuA"
                     /locus_tag="ACHJ48_004048"
     CDS             799..1758
                     /gene="ssuA"
                     /locus_tag="ACHJ48_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO4709495.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTAADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1755..2900
                     /gene="ssuD"
                     /locus_tag="ACHJ48_004049"
     CDS             1755..2900
                     /gene="ssuD"
                     /locus_tag="ACHJ48_004049"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO4709496.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVENDFIPRRVAQS"
     gene            2912..3703
                     /gene="ssuC"
                     /locus_tag="ACHJ48_004050"
     CDS             2912..3703
                     /gene="ssuC"
                     /locus_tag="ACHJ48_004050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO4709497.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVTVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3700..4467
                     /gene="ssuB"
                     /locus_tag="ACHJ48_004051"
     CDS             3700..4467
                     /gene="ssuB"
                     /locus_tag="ACHJ48_004051"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO4709498.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4510..7122)
                     /gene="pepN"
                     /locus_tag="ACHJ48_004052"
     CDS             complement(4510..7122)
                     /gene="pepN"
                     /locus_tag="ACHJ48_004052"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO4709499.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLCLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7388..8590
                     /gene="pncB"
                     /locus_tag="ACHJ48_004053"
     CDS             7388..8590
                     /gene="pncB"
                     /locus_tag="ACHJ48_004053"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO4709500.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLEIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8759..10159
                     /gene="asnS"
                     /locus_tag="ACHJ48_004054"
     CDS             8759..10159
                     /gene="asnS"
                     /locus_tag="ACHJ48_004054"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO4709501.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQTSKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTEAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2344 a         2647 c         2787 g         2592 t
ORIGIN      
        1 ttatagattt catgaatatt taaatattac ctgaacgagt aattcattga ttcaacatct
       61 aatgactatc caataaataa atatgcgtga tatataaaac cctttcttta gtttattttc
      121 agaaaaagat acacaaaatt tatatttggc attttcttgt ctctccgaca gactgagcga
      181 attgttatca atgttaacaa aacaagaaca attggttata aggagagagt atgcgtgtca
      241 tcaccctggc gggtagtcct cgctttcctt ctcgctccag ctccttgctg gaatatgcgc
      301 gggaaaaact aaatggcctg gatgttgagg tttatcactg gaatctgcaa aacttcgccg
      361 cggaagatct gctttatgct cgtttcgata gtccggcact ccataccttc accgaacagt
      421 tgcaacaggc tgacgggctg attgtcgcca cgcctgtgta taaagccgct tattccggtg
      481 cgctgaaaac cctgctcgac ctgctgccag aacgtgcgtt gcaaggcaaa gtggtgctac
      541 cgctggcgac gggcggtacg gtggcccatc tgctggcggt cgattatgcc cttaagccag
      601 ttttaagtgc gctgaaagcc caggagatcc tgcacggcgt gtttgccgat gactcacaag
      661 taattgatta ccatcacaaa ccccagttca cgccgaattt acaaacccgt cttgataccg
      721 cgctagaaac tttctggcag gcattgcacc gccgcgatgt tcaggttcct gatcttcatt
      781 ctctgcgagg taatgcccat gcgtaacatc attaaactgg cgctggtggg attgcttagc
      841 gtctctacga ttgtggttgc tgcagaatcc tcgcctgaag cgttacgtat aggctatcag
      901 aaaggcagta ttggtatggt attggcaaaa agccaccagt tactggaaaa acgctatccg
      961 caatcaaaaa tctcgtgggt ggagttcccc gctggcccgc aaatgcttga ggcgttaaac
     1021 gttggcagta ttgatctcgg cagtaccggg gatattccgc caatcttcgc ccaggctgcc
     1081 ggggctgatt tgctgtacgt gggcgtcgag ccgccgaagc caaaagccga ggtgattctg
     1141 gtggcagaaa acagcccgat caaaaccgca gccgatctta aaggccacaa agttgcgttc
     1201 cagaaaggtt ccagttcaca caatctttta ctgcgcgcac tacgccaggc cggacttaag
     1261 tttactgaca tccagcccac ttatctgacg ccagctgatg cccgcgccgc gttccagcaa
     1321 ggtaacgttg acgcctgggc tatctgggat ccctactact ccgctgcatt attacagggc
     1381 ggcgtgcggg tgttgaaaga cggcaccgat ctcaatcaaa ccggatcgtt ttatctggca
     1441 gctcgcccct atgcagaaaa aaacggcgct tttattcagg gtgtactggc aacctttagt
     1501 gaggccgatg cgttaacccg cagccagcgc gagcaaagta tcgctttact ggcaaaaacg
     1561 atgggcttac cggcaccggt gattgcctct tacttagatc atcgccctcc taccaccatc
     1621 aaaccggtta acgccgaggt tgccgcctta cagcagcaaa cggcagatct gttttatgaa
     1681 aatcgtctgg tgccgaaaaa agtcgatatt cgccaacgca tctggcagcc cactcaactg
     1741 gaaggaaaac aattatgagt ctgaatatgt tctggttttt accgacccat ggtgacgggc
     1801 attatctggg aacggaagaa ggttcacgcc cggttgatca cggttatctg caacaaattg
     1861 cgcaagcggc ggatcgtctt ggctataccg gtgtgctgat cccaacgggg cgctcctgcg
     1921 aagatgcgtg gctggttgcc gcatcgatga tcccggtgac gcagcggctg aagtttcttg
     1981 tcgccctgcg tcccagcgta acctcaccta ccgttgccgc ccgccaggcc gccacgcttg
     2041 accgtctctc aaatggacgt gcgttgttta acctggtcac aggcagcgat ccacaagaac
     2101 tggcaggcga cggcgtgttc cttgatcata gtgagcgcta cgaagcctcg gcggaattta
     2161 cccaggtctg gcggcgttta ttgctggggg aaaccgtcga tttcaacggt aaacatattc
     2221 atgtgcgggg agccaaactg ctcttcccgc cgattcaaca gccgtatcct ccgctttact
     2281 ttggcggatc gtcagacgtc gcccaggagc tggcggcaga acaggttgat ctctacctca
     2341 cctggggcga accgccggaa ctggttaaag agaaaatcga acaagtgcgg gcgaaagctg
     2401 ccgcgcatgg acgcaaaatt cgtttcggca ttcgtctgca tgtgattgtt cgtgaaacta
     2461 acgacgaagc gtggcaggcc gccgagcggt taatctcgca tcttgatgat gagactatcg
     2521 ccaaagcaca agccgcattc gcccgcacgg attccgtggg gcaacagcga atggcagcgc
     2581 tacataacgg caagcgcgac aatctggaga tcagccccaa tttatgggcg ggcgttggct
     2641 tagtgcgcgg cggtgccggg acggcgctgg tgggcgatgg tcctacggtc gctgcgcgaa
     2701 tcaacgaata tgccgcgctt ggcatcgaca gctttgtgct ttcgggctat ccgcatctgg
     2761 aagaagcgta tcgggtcggc gagttgctgt tcccacatct ggatgtcgcc attccggaaa
     2821 ttccccagcc gcagccgctg aatccgcaag gcgaagcggt ggagaatgat tttatccctc
     2881 gcagagtcgc gcaaagctaa ggaagaagat aatggcaacg ccagtgaaga agtggttatt
     2941 gcgcgttgcc ccctggtttt taccggtggg catcgtgacg gtgtggcaac tggcctcctc
     3001 ggttggctgg ttgtcgacgc gtattttgcc ctcaccggaa ggggtggtga tggcgttctg
     3061 gacgctctca gccagcggcg aattgtggca gcatctggcg atcagctcct ggcgggcgct
     3121 gattggtttt tcaattggcg gctcgctggg gttgattttg ggactgatta gcggactgtc
     3181 gcgctgggga gaaaggctgc tggatacctc aattcagatg ttgcgcaacg tgccgcatct
     3241 ggcgctgatc ccgctggtga ttttgtggtt tggcattgat gaatccgcaa aaatctttct
     3301 ggtggcgctc ggtacgctat tccccattta tatcaacacc tggcatggca tccgtaatat
     3361 cgatcgcggg ttggtggaga tggcgcgtag ctatggatta tccggcatac cgctgtttat
     3421 ccatgtgatc ctgcctggtg ccctgccctc aattatggtc ggcgtgcgtt ttgcgttggg
     3481 cctgatgtgg ctgacgctga ttgttgccga aaccatttct gccaattcag gcattggtta
     3541 tctggcgatg aatgcgcggg agtttttgca aacggacgtg gtggtagtcg ctattattct
     3601 ttacgccctg ctcggcaaac ttgctgacgt cagcgcgcag ctgttagagc gcctctggtt
     3661 gcgctggaac ccggcttatc atttgaagga ggccacggta tgaatactgc tcgtctgaac
     3721 cagggcacac cattgttgct caatgcagta agcaaacatt acgcggaaaa tatcgtcctg
     3781 aaccaactgg atttacatat tccggcaggt cagtttgtgg cggtggtggg ccgcagcggt
     3841 ggtggcaaaa gtaccctgct gcgcctgctg gcaggtctgg aaacgccaac cgctggcgat
     3901 gtgttagcgg gcaccacacc gctggctgaa attcaggaag acacgcgaat gatgtttcag
     3961 gatgcgcgtc tgctaccgtg gaaatcggtg attgataacg ttgggttagg ccttaaaggc
     4021 cagtggcgcg atgccgcgcg tcgggcgttg gctgcggtag gactggagaa tcgcgccggg
     4081 gaatggcctg ccgcactttc tggcgggcag aaacagcgag tggcgctggc aagagcgttg
     4141 attcatcgac cgggtttatt gttgcttgat gaaccgctcg gtgcgctgga tgccttaacg
     4201 cgactcgaga tgcaggattt gattgtgtca ctttggcagg agcacggttt taccgtactg
     4261 ttggtgacgc atgatgtgag cgaagcggta gcaatggctg atcgggtgtt gttaattgaa
     4321 gagggaaaaa ttggtctgga tttgacggtg gatattcccc gtccgcgccg gttagggtcg
     4381 gtgcgattag cggagctgga agcggaagtg ttgcaacggg tgatgcagcg tggtgaatct
     4441 gaaactcgcc tgagaaaaca ggggtaaaat ccccgaatgg cggcgctatt gccgccattc
     4501 ggggatttat taagccagtg ctttggtgat cttctcgtac agatcgccag atagattttc
     4561 cagccctttc aactgttcca gcgccgcgcg cattttctcc tgacgtttgg catcataacg
     4621 tttcaggcga atcagcggtt caatcaggcg tgaagctacc tgcgggttac ggctgttcag
     4681 gtcggtaagc atttccacca ggaactggta accgctccca tcttcggcat ggaacgctgc
     4741 cgggttgctg cccgcaaacg caccaatcaa cgaacgaatg cggttcgggt tgctcatggt
     4801 aaatgagcga tgctgcaaca ggccgcgcac cgtctccagc acattcgccg ccgggctggt
     4861 ggcttgcagg ataaaccatt tatccatcac cagaccatcc tgatgccact tgtcgtcgta
     4921 ctcctgcatc agcgcgtcac ggcaaggcag ctgtgcagca accgccgcag aaagcgctgc
     4981 cagcgcatcg gtcatattgt ttgcttcatg gtattgcttg ctcaccagca cgtcagccag
     5041 atgcgtttca ccaaaagcaa ggaagcgcag gcaggcatta cgcagagtgc gttttgcaat
     5101 atcttcatgc tcaacacggt actcgctctg gtaattcgcg ttgtaaatag ccagtagctc
     5161 atccgccagt tcagtcgcca gagtacgagt gagtgcttcg cgtacttcgg caatagcaat
     5221 cgggtcgatg atatcgaaca gttcagccat ttcattgaca gaaggcagcg tcaggatttc
     5281 tgccgccagc gccggatcaa tcttctcatc gagcagcacc gcgcggaaag cgtcagccac
     5341 atgtaccggc agagacagcg gctgcccttg ctgatgacgg gcgacgttca gcttgatgta
     5401 ggttgccagc aaactttgcg ccgcatccca acgggaaaaa tcattacgcg catgacgcat
     5461 caggaaggtc agttgctgat cgctccactt atattccagt ttcactggcg cagagaattc
     5521 gcacagcagc gcaggcaccg gctggaagta gacattatca aaaacaaagg tctgttccgc
     5581 ctgggtgacg ttcagcacgg aattcaccgg atgaccgcct ttctgcaacg ggatcacttt
     5641 gccttcgttg tcatacaatt cgatggcaaa cggaatatgc agcggctgtt tttctgcttg
     5701 atccggcgtg gctggcgtgc gctggctgat ggtcagggtg tactgctcgg tttccggatt
     5761 gtagtcgtct ttgacggtca caaccggcgt accggactgg ctgtaccaac ggcggaaatg
     5821 ggagagatcg acattcgacg catcttccat cgcctgaacg aaatcgtcgc aggtcgctgc
     5881 actgccatca tggcgctcga aatagagctg catccctttc tggaagtttt cttcgccaag
     5941 cagagtgtgg atcatacgaa tcacttccgc gcccttctcg taaacggtca gggtgtagaa
     6001 attgttcatc tcaatgacca tgtccgggcg gattgggtgc gccatcgggc tggcgtcttc
     6061 cgcaaactgc aatccgcgca tggtgcgcac attattgata cggtttaccg cgcgggaacc
     6121 aaggtcagag ctgaattcct gatcgcggaa gacggttaaa ccttctttca ggctgagctg
     6181 gaaccagtcg cggcaggtca ctcggttacc ggtccagtta tggaaatatt catggccgat
     6241 aacgcgttca atatcgaggt aatctttgtc ggtggcggta tcggtgcggg ccagcacata
     6301 tttggagtta aagatattca gacccttatt ctccattgcg cccatattga agaaatccac
     6361 cgcgacgatc atatagatgt cgaggtcata ctccaggcca aagcgttctt catcccattt
     6421 catggagttt ttcagcgagg tcatcgccca cggcgcgcga tcaaggttgc cgcgatcgac
     6481 gtacagctcc agtgctactt cgcgaccaga acgcgtggta aaggtatcgc gcagtacatc
     6541 aaagtcgcct gccaccagcg caaacaggta gcacggtttc gggaacgggt cctgccactg
     6601 tacccaatgg cgtccgtttt ccagttcgcc ttgcgcaacg cggttaccgt tggaaagcag
     6661 gaagggatat ttggttttat cggcaataat tttggtggta aaacgcgcca gcacgtccgg
     6721 gcggtcgaga taatacgtaa tatggcggaa accttcggct tcacactggg tgcaaagcgc
     6781 atcgcctgac tgataaagcc cttccagcgc cgtattcgcc gccgggctta tttcattaat
     6841 gatcttaagc gtaaaacgct ccggcaaatt actgatgacc agtgcgccct cttcttcttt
     6901 ccaggcggtc cacggctcat cattaatatg aacagaaacc agtttgaggt cttcgccgtt
     6961 gagacaaagc ggagcatctg atgcaccatg acggacagcc tggctgaccg cggtaacgac
     7021 cgtcttttgc gcgtcgaggt caaaggtcaa gtcaatatca gtaatctggt aatccggcgc
     7081 acgataatcg tgacggtatt tggcttgtgg ctgttgagtc ataaataacc tttagcatct
     7141 tttatagagt ctggtgttca gtctattcct gttgcgtaaa tcgcgctatg cagaatcttc
     7201 atcttttcag gtacaaacgc ctttattgct acatttttat aacatacacc gcgtaatgcc
     7261 atcgaccaga aaggtggcat atggtgtgat cggggttcaa taaattgcga aacaaggtat
     7321 actccagcag ttcctgaaga tgtttattgt actaaacgct cctgtacgag gacgctactg
     7381 cgcacctatg acacaattcg cttctcctgt tctgcactcg ttgctggata cagatgctta
     7441 taagttgcat atgcagcaag ccgtgtttca tcattattac gatgtgcatg tcgcggcgga
     7501 gtttcgttgc cgaggtgacg atctgctggg tatttatgcc gatgctattc gtgaacaggt
     7561 tcaggcgatg cagcacctgc gcctgcagga tgatgaatat cagtggcttt ctgccctgcc
     7621 tttctttaag gccgactatc ttaactggtt acgcgagttc cgctttaacc cggaacaagt
     7681 caccgtgtcc aacgataatg gcaagctgga tattcgttta agcggcccgt ggcgtgaagt
     7741 catcctctgg gaggttcctt tgctggcggt tatcagtgaa atggtacatc gctatcgctc
     7801 accgcaggcc gacgttgcgc aagccctcga cacgctggaa agcaaattag tcgacttctc
     7861 ggcgttaacc gctggtcttg atatgtcgcg cttccatctg atggattttg gcacccgtcg
     7921 ccgtttttct cgcgaagtac aagaaaccat cgttaagcgt ctgcaacagg aatcctggtt
     7981 cgtgggcacc agcaactacg atctggcgcg tcggctttcc ctcacgccga tgggaacaca
     8041 ggcacacgaa tggttccagg cacatcagca aatcagcccg gatctagcca acagccagcg
     8101 agctgcactt gctgcctggc tggaagagta tcccgaccaa cttggcattg cattaaccga
     8161 ctgcatcact atggatgctt tcctgcgtga tttcggtgtc gagttcgcta gtcggtatca
     8221 gggcctgcgt catgactctg gcgacccggt tgaatggggt gaaaaagcca ttgcacatta
     8281 tgaaaagctg gaaattgatc cacagagtaa aacgctggtt ttctctgaca atctggattt
     8341 acgcaaagcg gttgagctat accgccactt ctcttcccgc gtgcaattaa gttttggtat
     8401 tgggactcgc ctgacctgcg atatccccca ggtaaaaccc ctgaatattg tgattaagtt
     8461 ggtagagtgt aacggtaaac cggtggcgaa actttctgac agccctggca aaactatctg
     8521 ccatgataaa gcgtttgttc gggcgctgcg caaagcgttc gaccttccgc atattaaaaa
     8581 agccagttaa tatcatcagg gagctaatcg gctccctttt tttaccttta attccgaaat
     8641 ctttcgctgc atttgcgaat tctgcttgtc tgattgcaga tgccaggtaa cataggtatc
     8701 cccccattta cgggtggaca agtgtttatt ttttccgact attaacagag agaatattat
     8761 gagcgttgtg cctgtagccg acgtactcca gggccgcgta gccgttgaca gcgaagtcac
     8821 cgtacgcgga tgggtacgta cccgccgaga ttcaaaagct ggcatctcct tcctcgccgt
     8881 ttatgacggt tcctgctttg atcctgtaca ggctgtcatc aataattctc tgcccaatta
     8941 caatgaagac gtcctgcgtc tgaccaccgg ctgctcggtc attgtgacgg gtaaagtcgt
     9001 ggcgtcgccg ggccaggggc aacaatttga aattcagacc agcaaggttg aagttgctgg
     9061 ttgggttgaa gatccagaca cttacccgat ggcggcaaaa cgccacagca ttgagtatct
     9121 gcgtgaagtc gctcacctgc gtccgcgcac aaacctgatt ggtgccgtcg cgcgcgttcg
     9181 ccatacgctg gcgcaggcgc tgcatcgctt ctttaacgag cagggattct tctgggtttc
     9241 aacgccactg attactgcgt ctgataccga aggggctggc gaaatgttcc gcgtttctac
     9301 gctggatctg gaaaatctgc cgcgtaacga tcagggcaaa gtggatttcg acaaagactt
     9361 ctttggtaaa gagtctttcc tgaccgtatc tggccagttg aacggcgaaa cctacgcttg
     9421 cgcattatcc aaaatttata ccttcggccc gactttccgt gctgaaaact ccaacaccag
     9481 ccgtcacctg gcggaattct ggatgctgga gccggaagtg gcgtttgcta acctgaacga
     9541 tatcgcgggt ctggctgaag ccatgctgaa atatgtcttc aaagcggttc tcgaagaacg
     9601 cgctgacgac atgaaattct tcgctgaacg cgtagataaa gatgccgttt cacgtctgga
     9661 acgctttatt gaggccgatt tcgcgcaggt ggattacacc gaagcagtaa ccattctcga
     9721 aaactgcggc aggaagtttg aaaacccggt ttactggggc gtcgatctct cttctgagca
     9781 tgagcgttat ctggcggaag aacactttaa agcaccggta gtggttaaaa actatccgaa
     9841 agatattaaa gcgttctata tgcgcctcaa cgaagacggt aaaaccgttg cggctatgga
     9901 tgttctggct ccgggcatcg gtgagatcat tggtggctcc cagcgtgaag agcgtctgga
     9961 cgtgctggac gagcgtatgc tggaaatggg cctgaacaaa gaagattact ggtggtatcg
    10021 cgatctgcgt cgctacggta ctgttccgca ttccggtttc ggtcttggtt ttgaacgcct
    10081 gattgcttac gtaactggtg tgcaaaacgt gcgtgatgtg attccgttcc cacgaactcc
    10141 gcgtaacgcc agcttctaat ttagcgtcta caagagccag cacccgctgg ctctttttta
    10201 tcctttctgt caagttatct gtttgttaag tcaatcaatc tatttgcaac cccgccataa
    10261 cgatcatcct gttacggaat attacattgc aacatttacg cacaaaaaat aacccgcatt
    10321 cttattgcgg attagttttt tcttagctga tagcacaatt ttcatactat
//
LOCUS       DAZFDC010000146        10322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFDC010000146 DAZFDC010000000
VERSION     DAZFDC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.146"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(16..642)
                     /gene="upp"
                     /locus_tag="ACHJ48_004055"
     CDS             complement(16..642)
                     /gene="upp"
                     /locus_tag="ACHJ48_004055"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO4709502.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            967..2004
                     /gene="purM"
                     /locus_tag="ACHJ48_004056"
     CDS             967..2004
                     /gene="purM"
                     /locus_tag="ACHJ48_004056"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO4709503.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVNAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            2004..2642
                     /gene="purN"
                     /locus_tag="ACHJ48_004057"
     CDS             2004..2642
                     /gene="purN"
                     /locus_tag="ACHJ48_004057"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO4709504.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHPHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2813..4879
                     /gene="ppk1"
                     /locus_tag="ACHJ48_004058"
     CDS             2813..4879
                     /gene="ppk1"
                     /locus_tag="ACHJ48_004058"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO4709505.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4884..6425
                     /gene="ppx"
                     /locus_tag="ACHJ48_004059"
     CDS             4884..6425
                     /gene="ppx"
                     /locus_tag="ACHJ48_004059"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO4709506.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6464..8707)
                     /gene="pdeF"
                     /locus_tag="ACHJ48_004060"
     CDS             complement(6464..8707)
                     /gene="pdeF"
                     /locus_tag="ACHJ48_004060"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO4709507.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRTVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(8889..9041)
                     /locus_tag="ACHJ48_004061"
     CDS             complement(8889..9041)
                     /locus_tag="ACHJ48_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709508.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKSSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            9059..9250
                     /locus_tag="ACHJ48_004062"
     CDS             9059..9250
                     /locus_tag="ACHJ48_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO4709509.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            9493..10302
                     /locus_tag="ACHJ48_004063"
     CDS             9493..10302
                     /locus_tag="ACHJ48_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4709510.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRG"
BASE COUNT         2744 a         2472 c         2464 g         2642 t
ORIGIN      
        1 aattattttt attctttatt tcgtaccaaa gattttgtca ccggcatcgc cgaggcccgg
       61 aataatgtat ccgtgctcgt tcagtccctg atcaatcgat gcggtataca gttcgacgtc
      121 cgggtgcgct ttttccagcg cagcgatacc ttctggcgca gctaccagca ccagaacttt
      181 gatgctgctg cagcccgctt ttttcagcag gtcgatggtc gcaataacgg aaccaccggt
      241 tgccagcatt gggtcaacga tcagcgccat acgctcatcg atgttagaaa ccagtttctg
      301 gaagtacggt accggctcca gcgtttcttc attacggtac ataccgacaa cgctgatgcg
      361 cgcgctcgga acgttttcca gcacaccgtc catcatcccc agacccgcac gcagaattgg
      421 cacaacggta attttcttac ctttgatctg gtcgatttct accgggccgt tccagccttc
      481 gatagttact ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca ggctacccac
      541 ttcggaagcg agttcgcgaa agcgcttggt gctgatatct tgctcacgca tcagtcccag
      601 cttgtgtttg acgagtgggt gtttgacttc cacgatcttc atactctttc tcctttgagg
      661 ggcagccaca aaaaaaatcg acggattata cctcctttct tcaaggcggc aatattattt
      721 tcgttgactt tagtcaaaat gataacggtt tgagataaag ttattttata ttcaaatggt
      781 tatgaaagaa gattattcca tccgaaaact aacctttgcc ctggcacaag tcttctttcg
      841 ccgcgcgcct ggggaaaaga cgtgcaaaaa ggttgtgtaa agcagtctcg caaacgtttg
      901 ctttccctgt tagaattgcg ccgaatttta tttttctacc gcaagtaacg cgtggggacc
      961 caagcagtga ccgataaaac ctctcttagc tacaaagatg ccggtgttga tattgacgcg
     1021 ggtaatgctc tggttggaag aatcaaaggc gtagtgaaga aaacgcgtcg tccggaagtg
     1081 atgggcggtc tgggcggctt cggtgcgctg tgtgcattgc cgcaaaaata tcgtgaaccc
     1141 gtgctggttt ccggcactga cggcgtaggt accaagctgc gtctggcaat ggacttaaaa
     1201 cgtcacgaca ccattggtat tgatctggtc gccatgtgcg ttaatgacct ggtggtgcaa
     1261 ggtgcagaac cgctgttttt cctcgactat tacgcaaccg gaaaactgga tgttgatacc
     1321 gcttcagcgg tgatcagcgg cattgcggaa ggttgtctgc aatcgggctg ttcactggtg
     1381 ggtggcgaaa cggcagaaat gccggggatg tatcacggtg aggattacga tgtcgcgggt
     1441 ttctgcgtgg gcgtggtaga aaaatcagaa atcatcgacg gctctaaagt cagcgacggc
     1501 gatgtgctga ttgcactcgg ttccagcggt ccgcactcga acggctattc gctggtgcgc
     1561 aaaattcttg aagtcagcgg ttgtgatccg caaaccaccg aacttgatgg taagccatta
     1621 gccgatcatc tgctggcacc gacccgcatt tacgtgaagt cagtgctgga gttgattgaa
     1681 aaggtcgatg ttaatgccat tgcgcacctg accggcggcg gcttttggga aaacattccg
     1741 cgcgtattgc cagataatac ccaggcagtg attgatgaat cctcctggca gtggccggaa
     1801 gtgttcaact ggctgcaaac ggctggtaac gttgagcgcc atgaaatgta tcgcaccttc
     1861 aactgcggcg tcgggatgat tattgccctg cctgctccgg aagtggacaa agccctcgcc
     1921 ctgctcaatg ccaacggtga aaacgcgtgg aaaatcggta tcatcaaagc ctctgattcc
     1981 gaacaacgcg tggttatcga ataatgaata ttgtggtgct tatttccggc aacggaagta
     2041 atttgcaggc aattattgac gcctgtaaaa ccaacaaaat taaaggcacc gtacgggcag
     2101 ttttcagcaa taaggccgac gcgttcggcc ttgaacgcgc ccgccaggcg ggtattgcaa
     2161 cgcatacgct catcgccagc gcgtttgaca gtcgtgaagc ctatgaccgg gagttgattc
     2221 atgaaatcga catgtacgca cccgatgtgg tcgtgctggc tggttttatg cgcattctca
     2281 gcccggcatt tgtctcccac tatgccgggc gtttgctgaa cattcaccct tctctgctgc
     2341 cgaaatatcc cggattacat ccccatcgtc aggcgctgga aaatggcgat gaagagcacg
     2401 gtacatcggt gcatttcgtc accgatgaac tggacggtgg cccggttatt ttacaggcga
     2461 aagtcccggt atttgctggt gatacggagg atgacgtcac cgcccgcgtg caaacccagg
     2521 aacacgccat ttatccactg gtgattagct ggtttgccga tggtcgtctg aaaatgcacg
     2581 aaaacgccgc gtggctggat ggtcaacgtc tgccgccgca gggctacgct gccgacgagt
     2641 aatgccccgt agttaaagcg ccagctctgc cgctggcgtt tttcaattca cctgtaaatc
     2701 gcaagctcca gcagtttttt tccccctttt ctggcatagt tggacatctg ccaatattgc
     2761 tcgccataat atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg taatgggtca
     2821 ggaaaagcta tacatcgaaa aagagctcag ttggttatcg ttcaatgaac gcgtgcttca
     2881 ggaagcggcg gacaaatcta acccgctgat tgaaaggatg cgtttcctgg ggatctattc
     2941 caataacctt gatgagttct ataaagtccg cttcgctgaa ctgaagcgac gcatcattat
     3001 tagcgaagaa caaggctcca actctcattc ccgccattta ctgggcaaaa ttcagtcccg
     3061 ggtgctgaaa gccgatcagg aattcgacgg cctctacaac gagctattgc tggagatggc
     3121 gcgcaaccag atcttcctga ttaatgaacg ccagctctcc gtcaatcaac aaaactggct
     3181 gcgtcattat tttaagcagt atctgcgtca gcacattacg ccgattttaa tcaatcctga
     3241 cactgactta gtgcagttcc tgaaagatga ttacacctat ctggcggtgg aaattatccg
     3301 tggcgatacc atccgttacg cgcttctgga gatcccatca gataaagtgc cgcgctttgt
     3361 gaatttaccg ccagaagcgc cgcgtcgacg caagccaatg attcttctgg ataacattct
     3421 gcgttactgc cttgatgata ttttcaaagg cttctttgat tatgacgcgc tgaatgccta
     3481 ttcaatgaag atgacccgcg atgccgaata cgatttagtg catgagatgg aagccagcct
     3541 gatggagttg atgtcttcca gtctcaagca gcgtttaact gctgagccgg tgcgttttgt
     3601 ttatcagcgc gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac tgactatttc
     3661 ccgctacgac tccatcgtcc ccggcggtcg ttatcataat tttaaagact ttattaattt
     3721 ccccaatgtc ggcaaagcca atctggtgaa caaaccactg ccgcgtttac gccatatttg
     3781 gtttgataaa gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg atgtgttgct
     3841 ctattatcct tatcacacct ttgagcatgt gctggaactg ctgcgtcagg cctcgttcga
     3901 tccgagcgtg ctggcgatta aaatcaacat ttaccgtgtg gcaaaagatt cacgcatcat
     3961 cgactcgatg atccacgccg cacacaacgg taagaaagtc accgtggtgg ttgagctaca
     4021 ggcacgtttt gacgaagaag ccaacattca ctgggcgaag cgcctgaccg aagcaggcgt
     4081 gcacgttatc ttctctgcgc cggggctgaa aattcacgcc aaactgtttc tgatttcacg
     4141 taaagaaaac ggtgaagtgg tgcgttatgc acacatcggg accgggaact ttaacgaaaa
     4201 aaccgcgcgt ctttatactg actattcgtt gctgaccgcc gatgcgcgca tcaccaacga
     4261 agtacggcgg gtatttaact ttattgaaaa cccataccgt ccggtgacat ttgattattt
     4321 aatggtgtcg ccgcaaaact cccgccgcct gttgtatgaa atggtggacc gcgagatcgc
     4381 caacgcgcag caagggctgc ccagtggtat caccctgaag ctaaataacc ttgtcgataa
     4441 aggcctggtt gatcgtctgt atgcggcctc cagctccggc gtaccggtta atctgctggt
     4501 tcgcggaatg tgttcgctga tccccaatct ggaaggcatt agcgacaaca ttcgtgccat
     4561 cagtattgtt gaccgttacc ttgaacatga ccgggtttat atttttgaaa atggcggcga
     4621 taaaaaggtc tacctttctt ccgccgactg gatgacgcgc aatattgatt atcgtattga
     4681 agtggcgacg ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca tcatcgacat
     4741 attgttcagc gatacggtca aagcacgtta tatcgataaa gaactcagta atcgctacgt
     4801 tccccgcggc aatcgccgca aagtacgggc gcagttggcg atttatgact acatcaaatc
     4861 actcgaacaa cctgaataac cctatgccaa tacacgataa atcccctcgt ccgcaggagt
     4921 ttgctgcggt cgatcttggt tcaaacagtt ttcacatggt catagcccgt gtggtagatg
     4981 gtgccatgca gattattggc cgcctgaaac agcgggtgca tctggcggac ggcctggggc
     5041 cagataatat gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg tcgctgtttg
     5101 ccgaacggct acaagggttt tctcctgcca gcgtctgtat agttggtacc catacgctgc
     5161 gtcaggcgct gaacgccact gactttctga aacgtgcgga aaaggtcatt ccctacccga
     5221 ttgaaattat ttccggtaat gaagaagccc gtctgatttt tatgggcgtg gaacataccc
     5281 aaccggaaaa aggtcgcaaa ctggttattg atattggcgg cggttctaca gaactggtga
     5341 ttggtgaaaa tttcgaaccg attctcgttg aaagccgccg gatggggtgt gtcagctttg
     5401 cccagcttta tttccctggc ggggtcatca ataaagagaa ttttcagcgc gctcgcatgg
     5461 cggcagcaca aaaactggaa actttaacct ggcaatttcg tattcagggc tggaacgtgg
     5521 caatgggcgc ttccggtacc ataaaagccg cccatgaagt gttaatggaa atgggcgaga
     5581 aagacgggat aattaccccg gaacgtctgg aaaaactggt aaaagaagtt ttacggcacc
     5641 gtaatttcgc atcgctgagt ttaccaggtc tttccgaaga gcggaaaaca gtcttcgttc
     5701 ctggactagc gattttatgc ggtgtgtttg atgctttagc catccgtgaa ctgcgccttt
     5761 ctgacggggc gttacgcgaa ggcgtactgt atgaaatgga aggacgtttc cgtcatcagg
     5821 atgtgcgtag tcgcaccgcc agcagcctcg ccaaccagta tcacatcgac agcgaacagg
     5881 cccgacgggt gctggatacc actatgcaaa tgtacgaaca gtggcgggaa cagcaaccga
     5941 agctggcgca tccgcaactg gaggcgctac tgcgatgggc cgccatgctg catgaggtcg
     6001 ggttgaatat caaccacagc ggtttgcatc gtcactccgc ttatattcta caaaacagcg
     6061 acttgccggg ttttaatcag gaacagcagc tgatgatggc gacactggtg cgctatcacc
     6121 gtaaagcgat taaactcgac gatctgccgc gctttacctt gtttaagaag aaacagttcc
     6181 tgccactgat tcagctattg cgccttggcg tattactcaa caatcagcgt caggcaacca
     6241 ccacaccgcc aacattgacg ctgattactg atgacagtca ctggacactg cgtttcccgc
     6301 atgactggtt tagtcagaat gcgctggtac tgcttgatct ggaaaaggag caagaatact
     6361 gggaaggcgt ggctggctgg cggttgaaaa ttgaagaaga aagtacacct gaaatcgcag
     6421 cttaatgctt tgcgggccga cgagaaatgt cggcccgcat tattcaggca ctttcgcgaa
     6481 tgggttcgat ttcattcagc gtatcaatta acggttgcgg cttaccaata agataaccct
     6541 gcatataatc gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact
     6601 ctgccactac ccgcattttc ttcattcgcg ccaggtggca aatcgatgcc actatctgat
     6661 aatccagact atttgacaca atattgcgga taaaactgcc gtcaatttta agcagatcgg
     6721 cattcacatt tttaagccgc gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa
     6781 tctggcagcc taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca
     6841 gagcattact ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttatcca
     6901 gcaactgact gacttcaaca ggaaaacgag cctgacatac cgaggttgga gacagattaa
     6961 tagcaaaacg gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct
     7021 caatgaccca catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc
     7081 tatcggggcc gattagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa
     7141 catcaccacg cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg
     7201 cctgctgtag tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg
     7261 ccccgcgacg ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat
     7321 ttagctctcc cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga
     7381 cgccaatctg cggttgcatc ggcatgccat cccaaaagaa acgaaattgc ttgagatggc
     7441 tatccagtgc ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc gcgagatcgt
     7501 tacccgaaag ctgataaaca tcttcacctg gttccagcaa gggtgacagc cagtgagaaa
     7561 gtttttgctt gtattgaatc cgcagcatga tgccatagtt tttaaccagc atttccatgc
     7621 cagggatgcg taaataacaa agcgcagacc agggggcatc acgcaacgcg cgattcaggg
     7681 cgcgaacatt tggcagatga accaccgggt ccacatacgc aaggcgctga atgcgtctga
     7741 ctaccgttct ctgacgggtt gccagtacag ccatataatt gacaataaaa gagaatacca
     7801 gataactgga ggaggttatg gtcagctgcg tggtatagcc aggataaatg ggaatgtaat
     7861 tttgatagct gtggatgctg atcatcaaca cgaccgccca gagcagcgaa atcagcttat
     7921 aaccatagcg catcgctccc cacatcatca ggggcagcaa taatgacaag gtataattgg
     7981 tgctaaaaat tgtgcttttt tcatttaacg gcatgcataa cagcaacagt aaagcaccta
     8041 atgccagcag ccagagtgcg aactcttttt tggtgacttt gacatcaacc tgctgtttta
     8101 attgcgaata ataactacgt aaataaaacg gatttcgcac tacccgaatg atgaagtagc
     8161 acagcgggac accaatcaga ttgcccacca gtaaggcctg ataattgatt aaggtcccga
     8221 gattaaaagg catgacgcca accagatttt ctctgctcgc cagtaatcct acaaatgcag
     8281 caaactggaa aagtatcaga aacagcgttg caggaaacac aatctgccag aatatacgct
     8341 gggaaattaa acgggtatca ccatgtgaaa cgttgttacg ccggggagta aagaccctgt
     8401 aaccgcccca acagagaatg ataatgataa taaaattagc cgttaatgag agcgtttcgt
     8461 aaaaacccag ctctgcatat ttgcggacaa aaatccctaa ggcgatccct ggcaatgccg
     8521 cccagctgaa aaacatcatc atgctgatca tcagtgccag aggcagataa aagagaaaaa
     8581 cctcaccgga agaaatatgc gcgaaagtat taatgtgggc gaaaatgggc aagattaaag
     8641 aaggcaggaa cagcggaagc ccccaccatt tatcacgtat ttttatataa gttgcattca
     8701 gtttcataga tgctcagcag aatcccccac atcctgaagg aggtgtattc agacaggcat
     8761 cccacctgac ttcgaatgat gattattcat cactatagag agcattgatt ctaagtgtca
     8821 tatgaaagtt ccaattgata tatatcaaac aaaataaccc tgattaatga attattacgt
     8881 ttatcatgtt aattcatcat tattacatca tcattgtaaa taattaaatt aacttccata
     8941 acattaaaat atgtatccac tgacgatttt ttacataacg aagaattgac cattttgtcc
     9001 tgttgtgcct taatgtaagt accgtccacg gcgtgggaca tacttcaagg aaccttttgt
     9061 gagtcaggca accagtatgc gaaaacgaca ccgatttaac agtcgcatga cccgtatcgt
     9121 actgctcatc agctttatct tcttctttgg ccgttttatc tactcgtccg tcggtgcctg
     9181 gcagcaccat cagagcaaaa aagaagctca gcaatccaca ctctccgtcg aatcaccggt
     9241 acaacgttag cggttacctt ctccactttc acagaacata acggcgcttc gctgtcggat
     9301 gcttttgctc tttggaatta tcaaagcggc agatattctt tcatcttaaa ttatacgtct
     9361 ttgtcccgac tgatgtttat cctgtttggc tgcgaaataa atataaaatt aatatatatt
     9421 tatgccgtaa ataccaatat acttagcaaa ctatgtgatt tccatttcga ttgatttagt
     9481 gtttattgac gtatgtactg gattattaac gataatatcg agttctggcc tgagcaccga
     9541 aaattaatat cggtacataa cgccgatctt aacgtcgttc tgacaacgcc agccagtcgt
     9601 tgtttatcac tgctacttga agcttttcct gatgtggttg cacaacaaga ttttttcacc
     9661 agagtctggg aagaagaagg tatgcgtgtg cctactaaca cgttatatca gaacatatcc
     9721 attatcagac gcggatttcg cgctgttggt gatactaccc actcgcttat tgcaacagtg
     9781 ccgagaagag gattcaagat ccataatgac attaacatac aaaatcatgt aataaactca
     9841 tcaacagacg cacatacaca caatgcccca cctgccataa aagttaatgc gggatacaaa
     9901 gagagcattg gtggcgcaaa gaatttcaat aacaaaatcc tcaaacatat aaaaagttat
     9961 ctaattatgt tgagcgcatt tgtcataggc gcatattctg catattggct atggaataat
    10021 aatcaaccga agccattttt caaggattac aagactgttg cggaaattaa tggctgccat
    10081 tttaatgtaa cagaagatac aattgatggg ttgaaagagt tcgataaata taaaacacgg
    10141 atactggatt cggggattaa ctgcaaaaaa tatccgtggt tatacttccc tcttgcaaaa
    10201 tcctcaccgg ggatgattgt tatggcatgc aataaaaact ataaccaaca tgaagtggca
    10261 ggctgtttaa ccctgtctta ccgtgaggtt aatcgtggtt gattataaaa aaaaacttat
    10321 ta
//
LOCUS       DAZFDC010000147        10214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFDC010000147 DAZFDC010000000
VERSION     DAZFDC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.147"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(113..2686)
                     /gene="clpB"
                     /locus_tag="ACHJ48_004064"
     CDS             complement(113..2686)
                     /gene="clpB"
                     /locus_tag="ACHJ48_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO4709511.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="ACHJ48_004065"
     CDS             complement(2816..3547)
                     /gene="yfiH"
                     /locus_tag="ACHJ48_004065"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO4709512.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLNLTGEPYASKRADASYSNTPGRVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="ACHJ48_004066"
     CDS             complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="ACHJ48_004066"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO4709513.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4659..5396
                     /gene="bamD"
                     /locus_tag="ACHJ48_004067"
     CDS             4659..5396
                     /gene="bamD"
                     /locus_tag="ACHJ48_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO4709514.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5667..6008
                     /gene="raiA"
                     /locus_tag="ACHJ48_004068"
     CDS             5667..6008
                     /gene="raiA"
                     /locus_tag="ACHJ48_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO4709515.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6111..6236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6112..6159
                     /gene="pheL"
                     /locus_tag="ACHJ48_004069"
     CDS             6112..6159
                     /gene="pheL"
                     /locus_tag="ACHJ48_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO4709516.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6258..7418
                     /gene="pheA"
                     /locus_tag="ACHJ48_004070"
     CDS             6258..7418
                     /gene="pheA"
                     /locus_tag="ACHJ48_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO4709517.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="ACHJ48_004071"
     CDS             complement(7461..8582)
                     /gene="tyrA"
                     /locus_tag="ACHJ48_004071"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO4709518.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPALRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="ACHJ48_004072"
     CDS             complement(8593..9663)
                     /gene="aroF"
                     /locus_tag="ACHJ48_004072"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO4709519.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9873..>10214
                     /gene="yfiL"
                     /locus_tag="ACHJ48_004073"
     CDS             9873..>10214
                     /gene="yfiL"
                     /locus_tag="ACHJ48_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO4709520.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADEN"
BASE COUNT         2619 a         2701 c         2392 g         2502 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat cattactgga
      121 cggcgacaat ccggtcttca ttaacttcca ggcgaataac tttacccgga accaattcac
      181 cagacagtat ttgctgtgcc aacgggtttt cgatctgctg ctggatagca cgtttcaaag
      241 gacgtgcacc atagactgga tcgtaaccgt tctcgctcag cagtttcagc gcctcgtcag
      301 aaatgtggat ttcataacca cgctcttcca gacgtttgta cagacgtttc aactgaatct
      361 gcgcaatcga ggcaatgtgc tgttcgccca gcggatggaa gaccaccact tcatcgatac
      421 ggttaatgaa ctccggacgg aagttatggc ttaccacacc gagcaccagc tctttcatgt
      481 gcgcataatc cagttcaccg aagcgttcct gaatcagatc ggaaccgagg ttagaggtca
      541 taatgacgac cgtattacgg aagtcgaccg ttctcccttg cccgtcagtc agacgcccat
      601 catccagtac ctgcaacaga atattgaaga catccggatg cgctttttcc acttcatcca
      661 gcaggatgac ggaatacgga cgacgacgca ccgcttcggt caggtagcca ccttcttcat
      721 aaccgacata tcccggaggc gcaccaacca gacgagacac cgagtgtttc tccataaact
      781 cggacatatc gatacggacc atcgcctcgt cgctatcaaa cataaagttc gccagcgcct
      841 tacaaagctc tgttttcccc acaccagttg ggccgaggaa caggaatgaa ccaatcgggc
      901 gatttggatc cgccagcccc gcacggctac gacgaatagc gttagatacc gcatcaaccg
      961 cttcgttctg accaattacg cgatggtgca gttcttgctc catacgcagc agtttttcgc
     1021 gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc acttcagcaa
     1081 tttcggcgtc ggtcacttta ttacgcaaca gacgcatagt tttgccttcg agctgcgttg
     1141 cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca gacatccgcg
     1201 ccaagtcacc cacacggcga gcctgttcaa tagcgatttt cgcctgttcc agttccgctt
     1261 taatggtctg cgtaccagaa agcgatgcct tctctgcttt ccactcttct tctaactcgg
     1321 agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccagacgt tttttactgg
     1381 cttcatcaga ctctttcatt aacgcctgtt gttccagttt gagctggatg atacggcgat
     1441 cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg gatgctgctt
     1501 catcgatcag gtcgatggct ttatccggca gctgacggtc agcaatgtag cgatgagaca
     1561 acgtcgccgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc aattcgtaac
     1621 gttctttcag gccacgcaga atcgcaatgg tatcttcaac agaaggctcg gcaacaaaca
     1681 ctttctggaa acgacgttcc agcgcagcat ctttttcgat gtactggcga tattcgtcaa
     1741 gcgtcgtggc acctacgcag tgcaattcac cacgcgccag cgccggtttc agcatgtttc
     1801 cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat tcgtcgataa
     1861 acaggatgac gttgccttcc tgtttggcaa gatcgttaag cacgcctttt aaacgttctt
     1921 caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc gccagtaccc
     1981 ggcggccttt caacccttcc ggcacttcgc cgttgataat acgctgcgcc agaccttcaa
     2041 cgatggcagt tttaccgacg ccgggttcac caatcagtac cgggttattt ttagtacgac
     2101 gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc ggatcgagtt
     2161 tgccctgttc ggctcgttcg gtaaggtcga tggtatattt tttcaaagcc tgacgttggt
     2221 cttcagcacc ttgatcgttc acgctttcac ctccacgcat ttgttcaatc gcttgagtaa
     2281 tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg cgagactcaa
     2341 gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt tgcgccagct
     2401 tgtcgcaaag attaagaacg cgcaccagat cctgtgatgg ctggacatca ccaccagtac
     2461 cttcaacctg cggtaaacga tttaatgcct gattgatatc tgtgcgcaac tggccagcat
     2521 ttatgccagc ggatgttaat aaaggactaa ccgaaccccc ttcctgattc agcagggcgc
     2581 tcattaaatg aagtggttcg ataaattggt tgtcgtgccc aagtgcaagt gattgggcat
     2641 cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact cctcccataa
     2701 cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc aaggttattg
     2761 accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct ttaggttata
     2821 tcagccaaat gaaacttgcc atacgaccgg tggtcttgtc gcgacgataa gagaagaaag
     2881 tctcattttc cgtatatgta caacggtcgc cgccgaaaat ttgctcaaca cccacgttcg
     2941 ccagacgctg ccgggcaagc tgataaatgt ccgccagata cttatcacca tgctgaatga
     3001 aagctgtact cgcttcggcg tctactgcca taaacgcctc gcgaacctcc gcccccactt
     3061 caaacgcgcg tgggccaatt gccggcccta accaggcgag aatattttct ggattatcag
     3121 caaaacagga aactgtctct tccagcacgc ctgcgcacag tccacgccag ccagcatgag
     3181 cggcggcgac ttccgttccc gctcgattgc aaaacagcac agggaggcag tcggcagtca
     3241 tcactgcgca aaccctgccg ggcgtattgc tataagaggc atctgctcgt tttgaggcat
     3301 aaggttcgcc agtaagatta agcacatctt tgccgtgtac ctgctcaagc cagaccggtt
     3361 tagaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctcctcaacg tgatccgggt
     3421 tatcgccaca atgggcaccg aggttgagcg agtcatacgg gggcaagctc acgccgccga
     3481 tacgagtaga gctacaggcc gcaacacctt ttggcagcgg ccactgcggg acaatcagct
     3541 tactcataac cagtccactt catctttatg ctcttcgaaa tcggcgcgca tcacctcaat
     3601 cagctccacc atatcttgtg gaataggcgc atgccattcc atttcgatac cggagatcgg
     3661 gtgataaaga cgcagcatgg ttgcatgaag cgcctggcgg tcaaatttac gcagcgtgga
     3721 gataaacgct tccgaagcac cttttggcgg gcgcggacgg ccaccataaa ccggatcgcc
     3781 caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac gtccagtttc
     3841 cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat agtgagtcac
     3901 cgccggtttg cccatcggat gcaccgccat gtgagtacgt ttggtcgggt ggcggctgat
     3961 tggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt catactcacg
     4021 agtaatttca cgcctttgca aagattcgac taaacgcgtc tgagccggaa cggtttttgc
     4081 cacaaccatc aggccagtgg tgtctttatc cagacgatgg acgatgcctg cacgcggtac
     4141 atcagcaatg ggcggatagt aatgaagtaa cgcattcagt accgtgccat ccgggttacc
     4201 cgcgccagga tgtaccacca gatcgcgcgg tttattaatg acgatgatat cttcatcttc
     4261 atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt caatctcagc
     4321 gttgatggca acctgctcgc cacccaatac tttttctttc ggcttatcac aaagtttgcc
     4381 gttaaccagc acgcgctggt cgaggatcca ttcttttatt cgcgaacgtg aataatccgg
     4441 gaacatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg acaccgttgc
     4501 agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg gggttttacg
     4561 gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc aggaacagag
     4621 aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg aaatatctgg
     4681 tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag gaagaagtac
     4741 ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag gacggtaact
     4801 ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt ggtccgtatt
     4861 cgcagcaggt gcagctggat ctcatctacg cctactataa aaacgccgat ttgccgttag
     4921 cgcaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat atcgattatg
     4981 tcatgtacat gcgtggcctg accaatatgg cgctggatga cagtgcgctg caagggttct
     5041 ttggcgttga ccgtagcgat cgcgatcctc aacatgcacg agctgcgttt agtgactttt
     5101 ccaaactggt gcgcggctat ccaaacagtc agtacaccac cgatgccacc aaacgtctgg
     5161 tattcctgaa agatcgtctg gcgaaatatg aatactccgt ggccgagtac tatacagaac
     5221 gtggcgcatg ggttgccgtc gttaaccgcg tagaaggcat gttgcgcgac tacccggata
     5281 cccaggctac gcgtgatgcg ctgccgctga tggaaaatgc ataccgtcag atgcagatga
     5341 atgcgcaagc tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga
     5401 aacctgaaac acaaaacggc agcccttgag ctgccgtttt tttattctgt cagttgtgaa
     5461 actgaagcga tttagtcact atcgatctca tcaaatatgg ctcgctttga gatattcctc
     5521 aagtaaaaaa acacctcttc ctgcgatttc tcacaaaaaa gattcgttga caaaaagtga
     5581 caaaattatg agatttccat cacacatttt gacatcagga acggtatgct gaattcacca
     5641 agacgggaag acaagaggta aaatttatga caatgaacat taccagcaaa caaatggaaa
     5701 ttactccggc catccgccaa catgtcgcag accgtctcgc caaactggaa aaatggcaaa
     5761 cacatctgat taatccacat atcattctgt ccaaagagcc acaagggttt gttgctgacg
     5821 ccacaatcaa tacacctaac ggcgttctgg ttgccagtgg taaacatgaa gatatgtaca
     5881 ccgcaattaa cgaattgatc aacaagctgg aacggcagct caataaactg cagcacaaag
     5941 gcgaagcacg tcgtgccgca acatcggtga aagacgccaa cttcgtcgaa gaagttgaag
     6001 aagagtagtc ctttatattg agtgtatcgc caacgcgcct tcgggcgcgt tttttgttga
     6061 cagcgtgaaa acagtacggg tactgtacta aagtcactta aggaaacaaa catgaaacac
     6121 acaccgtttt tcttcgcatt cttttttacc ttcccctgaa tgggaggcgt ttcgtcgtgt
     6181 gaaacagaat gcgaagaaga acaataaggc ctcccaaatc ggggggcctt ttttattgat
     6241 aacaaaaagg caacactatg acatcggaaa acccgttact ggcgctgcga gagaaaatca
     6301 gcgcgctgga tgaaaaatta ttagcattac tggcagagcg gcgcgaactg gccgtcgagg
     6361 tgggaaaagc caaactgctc tcgcatcgcc cggtacgtga tattgatcgt gaacgcgatt
     6421 tactggaaag attaattacg ctcggtaaag cgcaccatct ggacgcccat tacattactc
     6481 gcctgttcca gctcatcatt gaagattccg tattaactca gcaggctttg ctccaacaac
     6541 atctcaataa aattaatccg cactcagcac gcatcgcttt tctcggcccc aaaggttctt
     6601 attcccatct tgcggcgcgc cagtatgctg cccgtcactt tgagcaattc attgaaagtg
     6661 gctgcgccaa atttgccgat atttttaatc aggtggaaac cggccaggcc gactatgccg
     6721 tcgtaccgat tgaaaatacc agctccggtg ccataaatga cgtttacgat ctgctgcaac
     6781 ataccagctt gtcgattgtt ggcgagatga cgttaactat cgaccattgt ttgttagtct
     6841 ccggcacgac tgatttatcc accatcaata cggtctacag ccatccgcag ccattccagc
     6901 aatgcagcaa attccttaat cgttatccgc actggaagat tgaatatacc gaaagtacgt
     6961 ctgcggcaat ggaaaaggtt gcacaggcaa aatcaccgca tgttgctgcg ttaggaagcg
     7021 aagctggcgg cactttgtac ggtttgcagg tactggagcg gattgaagcg aatcagcgac
     7081 aaaacttcac ccgatttgtg gtgttggcgc gtaaagccat taacgtgtct gatcaggttc
     7141 cggcgaaaac gacgttgtta atggcgaccg ggcaacaagc cggtgcgctg gttgaagcgt
     7201 tgctggtact gcgcaaccac aatctgatta tgacccgtct ggaatcacgc ccgattcacg
     7261 gtaatccatg ggaagagatg ttttatctgg atattcaggc caatcttgaa tcagcggaaa
     7321 tgcaaaaagc attgaaagag ttaggggaaa tcacccgttc aatgaaggta ttgggctgtt
     7381 accctagtga gaacgtagtg cctgttgatc caacctgatg aaaaggtgcc ggatgatgtg
     7441 aatcatccgg cactggatta ttactggcga ttgtcattcg cctgacgcaa taacacgcgg
     7501 ctttcactct gaaaacgctg tgcgtaatcg ccgaaccagt gctccacctt gcggaaactg
     7561 tcaataaacg cctgcttatc gccctgctcc agcaactcaa tcgcctcgcc gaaacgctta
     7621 tagtaacgtt tgattaacgc cagattacgc tctgacgaca taataatgtc ggcataaagc
     7681 tgcggatcct gagcgaacag tcgcccgacc atcgccagct caaggcggta aatcggcgaa
     7741 gagagcgcca gaagttgctc aagctgaaca ttttcttctg ccaggtgcag cccgtaagca
     7801 aaagtagcaa agtggcgcag tgcctgaata aacgccatat tctgatcgtg ctcgacggcg
     7861 ctaatacgat gcaaccgagc gccccagacc tgaatttgct ccagaaacca ttggtatgct
     7921 tccggtttac gtccatcaca ccagaccaca acttgctttg ccaggctacc gctgtccgga
     7981 ccgaacattg ggtgtaaccc cagtaccggg ccatcgtgcg ccgccagcat ggcctgtaat
     8041 ggtccatttt tcactgatgc cagatcaacc agaatacaat ctttcggtaa aggcggtaat
     8101 ttgccaataa cttgctcagt aacgtggatt ggcacactaa caatcaccat tccggcatcg
     8161 gcaacaatat cagccgctcg atcccagtca tgttgctcca gaatccgcac ctgataaccc
     8221 gagagtgtca gcatcttctc gaacagacgt cccatctgac cgccgccgcc aacgataact
     8281 accgggcgta acgcaggaca aagcgtttta aatcctttgt cgttttcact ggagtaagat
     8341 tcacgcatca cccgacgcaa aacatcctca atcagatctg gcggtacacc cagagcttcc
     8401 gcctctgcac gacgcgaggc caacatagat gcctctcgct ccggaacata aataggcagt
     8461 ccaaagcggc ttttcacctc gcccacttca gcaaccagtt ccagacgctt cgctaataaa
     8521 ttcagcagcg ctttatcgac ttcatcaatt tgatcgcgta atgcggtcaa ttcagcaacc
     8581 ataataaacc tcttaagcca cgcgagccgt cagctgcccg ttcagatcct gatgaatttc
     8641 acgcagcaag gcatcggtca tttcccagct aatgcaggca tcggttacgg atacaccgta
     8701 tttcatttca ctgcgcggtt gctcggaaga ctgattgccc tcgtggatat tactttcgat
     8761 catcagacca ataattgagc gattgccatc tttgatttga gcaaccacgg attctgccac
     8821 cgcaggctga cggcgataat ctttattgga attaccgtgg ctgcaatcta ccatcagaga
     8881 cgggcgcagt cccgcctgtt ccatctcttt ttcacattgc gcaacatccg cagggctata
     8941 gttcggcgct ttaccaccgc gcaggatgac atgcccgtcc ggattcccct gagtttgtag
     9001 caacgcaacc tgccctgcct ggttaatgcc aacaaaacgg tgcggctggg cggcggcgcg
     9061 catagcgtta attgctgttg ccagactgcc gtcggtgccg tttttaaagc caaccggcat
     9121 ggaaagcccg gaggccattt cacggtgagt ttgcgattcc gttgtacgag caccaattgc
     9181 tgaccagcta aacagatcgc ccaggtattg cgggctattc ggatctaacg cttccgtcgc
     9241 cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct gcagcccggc
     9301 ttctacatca aaagagccat ccatatgggg atcgttaatt aaccctttcc agccgacagt
     9361 ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc tgacctctgc
     9421 ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat gaatggaaca
     9481 agggccacat actaccagca gacgaggatc gcgcccggcg ataatatctg aaatggtttt
     9541 acgcgagtca gcaatctggg cttcttgttg caggcttaat ggaaaagcgg ccttcagttg
     9601 tgccggagtc attaaaacct gttcgtcggt aatatgtacg ttattcagcg cgtctttttg
     9661 catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga ggatgacgta
     9721 acgataacac ataaagtaaa gttttcaatc catatttcgt acatttttct ttacataagc
     9781 aatttattcg cgctttcaac ccttgattct gtatagataa atttacacca cctttgaaaa
     9841 caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaagtttatc gcccccttgt
     9901 tggctttact ggttagcgga tgccagattg atccttatac tcacgcgcca accttgacca
     9961 gcaccgactg gtatgatgtt ggtatggaag atgcgatatc gggcagcgcc ataaaagatg
    10021 acgatgcatt tagcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag
    10081 gacaaaagaa aacttgccag accgatttta cttatgcccg aggactttcc ggtaaaagct
    10141 ttcctgccag ctgcaacaat gttgaaaatg ccagccaact gcatgaagta tggcaaaaag
    10201 gggctgatga aaat
//
LOCUS       DAZFDC010000148        10196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFDC010000148 DAZFDC010000000
VERSION     DAZFDC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.148"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..68
                     /locus_tag="ACHJ48_004074"
     CDS             <1..68
                     /locus_tag="ACHJ48_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709521.1"
                     /translation="PEGKLYFALMNVLQMFPMKNG"
     gene            193..570
                     /locus_tag="ACHJ48_004075"
     CDS             193..570
                     /locus_tag="ACHJ48_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709522.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKNARLELALICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
     gene            686..1486
                     /gene="ydcF"
                     /locus_tag="ACHJ48_004076"
     CDS             686..1486
                     /gene="ydcF"
                     /locus_tag="ACHJ48_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO4709523.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDVPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            1683..3122
                     /gene="aldA"
                     /locus_tag="ACHJ48_004077"
     CDS             1683..3122
                     /gene="aldA"
                     /locus_tag="ACHJ48_004077"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO4709524.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3164..4165)
                     /gene="gap"
                     /locus_tag="ACHJ48_004078"
     CDS             complement(3164..4165)
                     /gene="gap"
                     /locus_tag="ACHJ48_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO4709525.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYKVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            4354..4884
                     /gene="cybB"
                     /locus_tag="ACHJ48_004079"
     CDS             4354..4884
                     /gene="cybB"
                     /locus_tag="ACHJ48_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO4709526.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            5129..5302
                     /locus_tag="ACHJ48_004080"
     CDS             5129..5302
                     /locus_tag="ACHJ48_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709527.1"
                     /translation="MKKLALILFMGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            5328..5396
                     /gene="yncP"
                     /locus_tag="ACHJ48_004081"
     CDS             5328..5396
                     /gene="yncP"
                     /locus_tag="ACHJ48_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO4709528.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(5374..5523)
                     /gene="hokB"
                     /locus_tag="ACHJ48_004082"
     CDS             complement(5374..5523)
                     /gene="hokB"
                     /locus_tag="ACHJ48_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO4709529.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDSDKEVAALM
                     ACTSR"
     gene            5922..7562
                     /gene="trg"
                     /locus_tag="ACHJ48_004083"
     CDS             5922..7562
                     /gene="trg"
                     /locus_tag="ACHJ48_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO4709530.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHYA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKQSVLAEPRGAGEPVSFAPV"
     gene            complement(7600..8523)
                     /gene="ydcI"
                     /locus_tag="ACHJ48_004084"
     CDS             complement(7600..8523)
                     /gene="ydcI"
                     /locus_tag="ACHJ48_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO4709531.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            8740..10083
                     /gene="ydcJ"
                     /locus_tag="ACHJ48_004085"
     CDS             8740..10083
                     /gene="ydcJ"
                     /locus_tag="ACHJ48_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO4709532.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
BASE COUNT         2653 a         2340 c         2588 g         2615 t
ORIGIN      
        1 tgcctgaggg gaaattgtat ttcgcattaa tgaatgtatt gcagatgttt cccatgaaaa
       61 atggttaata actggacaag ataattttaa aattcagcaa cggttatcga ttatacatgg
      121 tacttaaaca gactcacacc atttttgtag aaggttaaat tgagtacagt ctcatatatt
      181 aagggtgtta acatggatga acgttttata aatatcacgc cgacttcgtt gtcttttaaa
      241 cacgccgatc tgtcaggttt aagctataag aatgccaggc ttgaactggc cctgatatgc
      301 ccggcgatca agtcagaagt taagatatca tttgactgga tacactcatt tcgtgttact
      361 gacgaaggtg atttgttagg tatgtcagga aagcagggaa ttaaccaaac ggggatctat
      421 agagtagcca attccactta tcttacatgg tttactatgc aaagttgtga aattcataaa
      481 aataaaatga tcgaacatta tgttattgcc acgtcaaatg atgttattga tgttttatca
      541 acagtttctc cgcaaatagt gtatgactaa aaccagtgtc tggttcgcga tggcgagata
      601 gaaaaagcat tatcttcctt tcagagtgct ccgcttctca ctattatctc acgcagtatt
      661 cttaagggaa cgataaggag gaaccatgaa cattaccccg tttccgacgc tttcgacggc
      721 aactatagat gccataaatg ttatcggaca gtggctggcg caggatgatt tctccggtga
      781 ggtgccgtat caggctgatt gcgtcatact tgcaggaaat gcggttatgc cgactatcga
      841 tgcggcatgt aagattgctc gcgatcagca aattccttta ctgattagtg gtggtatcgg
      901 tcactcgacc acttttttgt atagcgccat cgcacagcat ccgcactaca acactatccg
      961 caccactggc agagcagaag cgaccatcct ggcggatatc gctcatcagt tctggcatat
     1021 tccgcatgaa aaaatctgga ttgaagacca gtcaacaaac tgcggtgaaa acgcacgctt
     1081 tagcatcgcg ctattgaatc aggccgtaga acgagttcac acagctatcg ttgtacagga
     1141 ccccaccatg cagcggcgca cgatggcgac gttccgccgt atgaccgggg acaatcccga
     1201 tgtaccacgc tggttaagtt atcccggatt cgttcctcag ttaggaaata acgcagacag
     1261 tgtaatcttt attaatcagt tacaaggatt atggccggtt gagcgttatc tctcactact
     1321 cactggcgag ctgccgcgtt tacgcgatga tagcgatggc tacggtcccc gcgggcgaga
     1381 ttttatcgtt cacgttgatt ttccggcaga agtcatccat gcatggcaaa cgctgaaaca
     1441 cgatgcggtg ctcatcgagg cgatggaaag tcgctcgtta cgttaaaaat tgcccgtttg
     1501 tgaaccactt gtttgcaaac gggcataact cctgcctttt atttctgcct cttattcctt
     1561 ttacacttgt ttttatgaag cccttcacag aattgtcctt tcatgatgcc gtctctatga
     1621 tgattgatgt taattaacaa tgtatttgcc aaaaacaaac atataaatca caggagtcgc
     1681 ccatgtcagt acccgttcaa catcctatgt atattgatgg acagtttgtt acctggcgtg
     1741 gagacgcatg gattgatgtg gtaaaccctg ctacagaggc tgtcatttcc cgcattcccg
     1801 atggtcaggc cgaggatgcc cgtaaggcaa tcgatgcagc agaacgtgca caaccagaat
     1861 gggaagcgtt gcctgcaatt gaacgcgcca gttggttgcg caaaatctcc gccgggatcc
     1921 gcgaacgcgc cagtgaaatc agtgcgctga ttgttgaaga agggggcaag atccagcagc
     1981 tggctgaagt cgaagtggct tttactgccg actatatcga ttacatggcg gagtgggcac
     2041 ggcgttacga gggcgagatt attcaaagcg atcgtccagg agaaaatatt cttctgttta
     2101 aacgtgcgct tggtgtgact accggcattc tgccgtggaa cttcccgttc ttcctcattg
     2161 cccgcaaaat ggctcccgct cttttgactg gtaataccat tgtcattaaa cccagtgaat
     2221 ttacgccaaa caatgcgatt gcattcgcca aaatcgtcga tgaaataggc cttccgcgcg
     2281 gcgtgtttaa ccttgttctg gggcgtggtg aaaccgttgg gcaagaactg gcgggtaacc
     2341 caaaggtcgc aatggtcagt atgacaggca gcgtctctgc aggtgagaag atcatggcga
     2401 ctgcggcgaa aaacatcacc aaagtgtgcc tggaactggg gggtaaagca ccagctatcg
     2461 taatggacga tgccgatctt gaactggcag tcaaagccat cgttgattca cgcgtcatta
     2521 atagtgggca agtgtgtaac tgtgcagaac gtgtttatgt acagaaaggc atttatgatc
     2581 agttcgtcaa tcggctgggt gaagcgatgc aggcggttca atttggtaac cccgctgaac
     2641 gcaacgacat tgcgatgggg ccgttgatta acgccgcggc gctggaaagg gtcgagcaaa
     2701 aagtggcgcg cgcagtagaa gaaggggcga gagtggcgtt gggtggcaaa gcggtagagg
     2761 ggaaaggata ttattatccg ccgacattgc tgctggatgt tcgccaggaa atgtcgatta
     2821 tgcatgagga aacctttggc ccggttctgc cggtagtcgc atttgacacg ctggaagatg
     2881 ctatctcaat ggctaatgac agtgattacg gcctgacctc atcaatctat acccaaaatc
     2941 tgaacgtcgc gatgaaagcc attaaagggc tgaagtttgg tgaaacttac atcaaccgtg
     3001 aaaacttcga agctatgcaa ggcttccacg ccggatggcg taaatccggt attggcggcg
     3061 cagatggtaa acatggcctg catgaatatc tgcagaccca ggtggtttat ttacagtctt
     3121 aatgagtgaa agaggcggag gttttttcct ccgcctgtgc gcgtcagagt ttagcgaatt
     3181 tttcgagggt gcgaataagc tgcgtgacga agccatattc gttgtcgtac caggcgaccg
     3241 ttttcaccag ttgtaaatcg cccacggcgg taatttccgt ttgcgtggca tcaaacaccg
     3301 aaccgaaatg gctgccaatg atatcggaag agactatttc ttcatcggta taaccaaatg
     3361 actcgttatt ggtggttgct tgtttaagtg cgttattcac ctcttcggca gtcacttttt
     3421 ttccgagaat cgataccagt tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     3481 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcgcccg
     3541 tagtgtgggg aatgatattt tctgccgcgg cgcgtgaagc acgtaaatct ttaccacgcg
     3601 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     3661 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aagacagtta gtagtgcatg
     3721 atgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattgact ttataaacga
     3781 tagttttcat ttcacctgca ggggcggaaa tcaacacctt cttcgcgcca gcatcaagat
     3841 gcgcctgcga tttctcggcg gaggtataaa agccagtaca ttcgacaatg atttctgcac
     3901 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3961 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     4021 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     4081 cgtctatgtt gcttttgact tcaagtaatc gacgcaacac cagtcgaccg atacgaccaa
     4141 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     4201 gatcctttcc aggcttagac caggatggcg ggatgggcaa tctccattct tacagtgaaa
     4261 cgtaacgtac tgaaaaaggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     4321 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     4381 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     4441 cggggtttct tcccacgtag cgatcggcca ctcatcaaca tgattcatgt ttcctgcggc
     4501 atatcaattc tcgtgctgat ggtcgttcgt cttttgttaa ggctgaaata cccaaccccg
     4561 ccgattatac ctaagccaaa accgatgatg acgggactgg cgcatttggg acatttggtg
     4621 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccgaggc
     4681 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     4741 gtagatagct taaagtcgtg gcatgaaacg ctggcgaatc tgggatattt tgtcatcggt
     4801 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     4861 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     4921 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4981 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     5041 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgaac
     5101 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     5161 agcgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     5221 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     5281 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     5341 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     5401 agcaacctcc ttatcactgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     5461 tgtgaatgtc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     5521 catagccttt ctccttgccg gatggcgggt aagaggctaa catctgaatt gcttggttca
     5581 ttcgttggcc tcggttgata gaaatatcgg tcggggcctt cgtctttctg attcccggtt
     5641 agcctgaaaa cagaaagtct caggcacccg tgagcatcct atgtggtttc cttagggacg
     5701 aaaataatca cctgacgaaa ttgcgtgctg ttttccagaa tttttcgtca ttcgggttag
     5761 ccagtttaac cattcgttac tctcttcatt ccaatggcat taatcttctc tgcaataatt
     5821 gttgtaaaaa tgtgacgcaa agaggctttt agtcgtaagt aattaccgtc aaatgccgat
     5881 gactttctat caggagtaaa cctggacgag agacaacggt aatgaataca actccctcac
     5941 agcgattagg ttttttgcat cacatcaggt tggttccgtt atttgcctgc attctaggcg
     6001 gtatcttagt tctattcgca ttaagttcag ccctggctgg ctatttcctc tggcaggccg
     6061 atcgcgatca gcgtgatgtt actgcggaga ttgagattcg gaccgggtta gcgaacagtt
     6121 cagatttttt gcgttcagcc cggatcaata tgattcaggc cggggctgcg agtcgtattg
     6181 cggaaatgga agcaatgaag cgaaatattg cgcaagccga atcggagatt aaacagtcgc
     6241 agcaaggtta tcgtgcttat cagaatcggc cggtgaaaac acctgctgat gaagccctcg
     6301 acactgaatt aaatcaacgc tttcaggctt atatcacggg tatgcaacct atgttgaaat
     6361 atgccaaaaa tggcatgttt gaagcgatta tcaatcatga aagtgagcag atccgaacgc
     6421 tggataatgc ttataccgat attttgaaca aagccgttaa gatacgtagc accagagcca
     6481 accaactggc ggaactggcc catcagcgca cccgcctggg cgggatgttc atgattggtg
     6541 cgtttgtgct tgccctggtc atgacgctga taacatttat ggtgctacgt cggatcgtca
     6601 ttcgtccact gcaacatgcc gcacaacgga ttgaaaaaat cgctagtggc gatctgacga
     6661 tgaaggatga accggcgggt cgtaatgaaa tcggtcgctt aagtcgtcat ttacagcaaa
     6721 tgcagcattc actggggatg accgtaggga ctgttcgaca gggtgcggaa gagatttatc
     6781 gtggcaccag cgaaatttca gctggcaatg cggacctgtc atctcgcacc gaagaacaag
     6841 cggcggctat cgaacaaact gccgccagca tggagcaact cactgcgacg gtgaaacaga
     6901 atgcggataa cgcgcattat gccagcaaac tggcgcaaga ggcttctatt aaagccagcg
     6961 atggcgggca gacggtttcc ggtgtagtaa aaacgatggg cgctatctcc acgagttcga
     7021 agaaaatttc tgagatcacc gccgtcatca acagtattgc tttccagacg aatattctgg
     7081 cactgaatgc tgccgttgaa gctgcgcgag cgggtgagca agggcgtgga tttgccgttg
     7141 tcgccagcga agtacggaca ctcgcaagcc gcagcgccca ggcggcaaaa gaaattgaag
     7201 gcttgatcag tgaatcagtc aggttaattg acctgggatc ggatgaggtg gcaacggccg
     7261 ggaaaaccat gagcactatt gttgatgccg tcgcgagtgt cacacatatc atgcaggaaa
     7321 ttgccgccgc ctcggatgag caaagtaggg gcataacgca ggttagccag gcgatttctg
     7381 aaatggataa ggtgacgcaa cagaatgctt ctctggtaga agaggcctca gcggcggcgg
     7441 tgtcccttga agaacaggcg gcacgattaa ctgaggcggt ggacgtattc cgtctgaaca
     7501 aacaatctgt gttggcagaa cctcgcggag cgggtgaacc agttagtttc gctccggtgt
     7561 gaaaatgtta aaggagggat cgacagatcc cttcaccttt cagaacggca ttgattttcg
     7621 aatagcgtta atcatcaact ggcaaccaga agagaacgtc gcatctacgc gggtcagtat
     7681 tccaatcggt tcgcctgcac catgtcccgg aacaggcagg gccaccagcg tggcatgacg
     7741 caggtcgtct tttaccgcgc cagaagggac aaaccacacg tagtcgtatt caaccgtaag
     7801 ttgacgagat agcgaagcag acagcgtttc gatacaaccc gaaggaattt tacatccctg
     7861 gctctgtact aatgcatctg aatgctggcg tggcgcagtg ccttctggtg atacaacgac
     7921 cggccattcc agcacccggc ttagcgttac gttctcctga agtagcggat gattagggcg
     7981 gacaaccagc ttcagcgatt caagaaacag cagttcgtaa ttaagcccgg tcatcagttc
     8041 aggatctgac atccgaccaa tgccgatatc gatttccccg gttttcaaac ccgccagaat
     8101 catagggtta ctcattgtcg caacttgcaa ggtcgtctct ttttgttgct gatgaaactg
     8161 acctataacc gaaggtaata tccccagtgc cgcagtaggt agtgcaccaa ccctgacgac
     8221 atcattatta ggaccttctt tacgatgaag cgactgtccg gcagtgttga tggcgtcaag
     8281 tactctgact gcatgcgtta aaaattgttc gccgggtaag gtaagttgcg ccccctgacg
     8341 accacgctca aacaagcgag cgcccgtcag ctgctccagt tcatttaatg tcttagagag
     8401 cgcaggttga ctcaaattaa gggtttcagc cgcgcgcccc aaagttcctt gttgtgcgac
     8461 agctacgaat gtatgaaggt ggcgcaaacg gatgcgctga ctaaacagac tatttttttc
     8521 cataagcgat gttaaaaacg aagcggtgtc gctgacaagt gaagttgttt gattatgata
     8581 acttgattgc aaaatattat taacaattaa agcaattatg ttacagcaaa atggataata
     8641 ttgatgtttt cgcggcgaga tcacagtttg taaattcttc ccgccagagt gaatgcggtt
     8701 acctacactc cagattactg accactggag gcagacacta tggcgaacag catcacggcg
     8761 gatgagattc gggaacagtt ttcgcaggca atgtcagcca tgtaccagca agaagttccg
     8821 caatatggca cgctgctgga actggtagct gatgtgaatc tggctgtgct ggaaaacaat
     8881 cctcaactgc acgaaaaaat ggtaaatgca gacgagctgg cgcgactgaa tgttgaacgt
     8941 catggggcga ttcgcgttgg gactgcacaa gagcttgcta ctcttcggcg gatgtttgcc
     9001 attatgggga tgtacccggt gagctattac gatctctcgc aggcaggggt gccggtacat
     9061 tcgacagcat ttcggcccat tgatgatgct tctctggcgc gtaatccctt ccgcgttttt
     9121 acctccttac tccgccttga gcttatcgag aacgaaattt tgcgccagaa agcggcggag
     9181 attctgcgtc agcgcgatat cttcacccca cgttgtcgac aactgttaga ggagtatgac
     9241 cagcggggcg gttttaacga aacacaggca caggagtttg tgcaggaggc cctggaaacg
     9301 ttccgttggc accagtcagc aacggtagat gaagaaacct atcgcgcctt gcacaacgaa
     9361 catcggttga ttgctgatgt ggtctgtttt cctggatgcc atatcaacca tctgacgcca
     9421 cgtacgctgg atattgaccg ggtgcagtcg atgatgcctg aatgcggaat tgaaccaaaa
     9481 attctgatcg aagggccgcc gcgccgcgag gtaccgattt tactacgcca gaccagcttt
     9541 aaagcactgg aagagacggt gttgtttgcg gggcagaaac agggcacgca tactgcgcgc
     9601 tttggtgaaa ttgagcagcg tggcgtggca ttaacgccga aaggtcgaca actgtatgat
     9661 gatcttctgc gtaacgctgg aaccgggcag gataatctca ctcaccaaat gcatttacag
     9721 gaaaccttcc gcacttttcc tgacagtgag tttttaatgc gtcagcaagg gctggcatgg
     9781 ttccggtacc gtctgacgcc ttcaggtgag gcgcatcgtc aggcgattca tcccggagac
     9841 gatccacagc ccttaattga acgtggttgg gtcgcggcgc aacccattac ctatgaagat
     9901 ttcttgcccg ttagcgcggc ggggatcttc cagtcaaatc tgggtaatga aacgcaggca
     9961 cgcagccacg gtaatgccag tcgcgaagca tttgagcagg cgttgggttg tccggttttg
    10021 gatgagttcc agctttatca ggaagcggaa gaacgcagta aacgtcgctg tggtttgctt
    10081 taaattctga ccatccgcct ttgcaaaaat ttgcctgatt ttacaaacga atcaggctca
    10141 tcccatcgac ataaaaaaat tgccgattta tgcatattct ctcagttcaa caattg
//
LOCUS       DAZFDC010000149        10021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFDC010000149 DAZFDC010000000
VERSION     DAZFDC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.149"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            379..1356
                     /gene="glaH"
                     /locus_tag="ACHJ48_004086"
     CDS             379..1356
                     /gene="glaH"
                     /locus_tag="ACHJ48_004086"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO4709533.1"
                     /translation="MNALTAVQNNAVDSDQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPMARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIEISKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1376..2644
                     /gene="lhgO"
                     /locus_tag="ACHJ48_004087"
     CDS             1376..2644
                     /gene="lhgO"
                     /locus_tag="ACHJ48_004087"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO4709534.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2667..4115
                     /gene="gabD"
                     /locus_tag="ACHJ48_004088"
     CDS             2667..4115
                     /gene="gabD"
                     /locus_tag="ACHJ48_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO4709535.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPVWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4129..5409
                     /gene="gabT"
                     /locus_tag="ACHJ48_004089"
     CDS             4129..5409
                     /gene="gabT"
                     /locus_tag="ACHJ48_004089"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO4709536.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5647..7047
                     /gene="gabP"
                     /locus_tag="ACHJ48_004090"
     CDS             5647..7047
                     /gene="gabP"
                     /locus_tag="ACHJ48_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO4709537.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7068..7730
                     /gene="csiR"
                     /locus_tag="ACHJ48_004091"
     CDS             7068..7730
                     /gene="csiR"
                     /locus_tag="ACHJ48_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO4709538.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7731..8180)
                     /gene="kbp"
                     /locus_tag="ACHJ48_004092"
     CDS             complement(7731..8180)
                     /gene="kbp"
                     /locus_tag="ACHJ48_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO4709539.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8264..8422)
                     /gene="yqaE"
                     /locus_tag="ACHJ48_004093"
     CDS             complement(8264..8422)
                     /gene="yqaE"
                     /locus_tag="ACHJ48_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO4709540.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8605..8904
                     /gene="ygaV"
                     /locus_tag="ACHJ48_004094"
     CDS             8605..8904
                     /gene="ygaV"
                     /locus_tag="ACHJ48_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO4709541.1"
                     /translation="MTELTQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8914..9432
                     /gene="ygaP"
                     /locus_tag="ACHJ48_004095"
     CDS             8914..9432
                     /gene="ygaP"
                     /locus_tag="ACHJ48_004095"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO4709542.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(9485..9889)
                     /gene="stpA"
                     /locus_tag="ACHJ48_004096"
     CDS             complement(9485..9889)
                     /gene="stpA"
                     /locus_tag="ACHJ48_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO4709543.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2296 a         2561 c         2732 g         2432 t
ORIGIN      
        1 ttagggaaaa caggagaacc gctgaactat ctctatttgt aatttaatgt tatatctgcc
       61 ccgctaaaac ggggcagata atatatttag tttactagcg gtcattttgc agtgaagcca
      121 tttaccgttt tttatcgacc agataatctg ttctctgatg gtaatccccc cctaacctgt
      181 tgctttagtt attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacca
      241 atttgttgct ttttatcaca ataagaaaac aatatggcgc atttgtgcgc atttttcaga
      301 aatgtagata tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc
      361 ataagaggat cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca
      421 gaccaggact atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa
      481 ctcaccttca ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg
      541 caggcgctgg agtacaaatc gtttctgcgt tttcgggtag gtaaaattct tgacgatctg
      601 tgtgcgaacc agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg
      661 ctgttgatca atgcggtggg tgtcgatgat gtgaagcaag cggatgagat ggtgaagctg
      721 gcgacggcgg tggcgcatct gattggccgt tccaatttcg atgcgatgag cggtcagtat
      781 tacgcgcgtt ttgtagtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac
      841 cgcgtaatgg agctgcacaa cgacggcacc tacgtcgaag agatcactga ctatgtgctg
      901 atgatgaaaa tcgacgagca gaacatgcag ggtggaaatt cgttgctgct gcatctcgat
      961 gactgggaac atctggacca ctatttccgc catccaatgg cgcgtcgccc gatgcgcttt
     1021 gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc atccagtgtt cgacgttgat
     1081 caacagggtc gcccggtgat gcgctatatc gaccagttcg tccagccaaa agacttcgaa
     1141 gaaggcgtgt ggttgagcga gctttcggac gccattgaaa tcagtaaagg cattctttct
     1201 gtacccgttc ccgttggcaa attcctgttg attaacaacc tgttctggct gcacggtcgc
     1261 gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga tgcgtcagcg tggctatttc
     1321 gcttacgcca ctcaccacta tcagacgcat cagtaagcgc aaaggaattg agcggatgta
     1381 tgattttgtg attattggcg gcggcatcat cggcatgtcg accgccatgc aactgattga
     1441 tgtctatccg gatgcccgca ttgcgttgct ggaaaaagag tccggcccgg cctgtcatca
     1501 gacgggccac aacagcggcg tgatccatgc cggggtttat tacacgcccg gtagcctgaa
     1561 ggcgcagttt tgcctggcgg gaaaccgcgc cactaaagcc ttttgcgatc aaaacggcat
     1621 tcgctatgac aactgcggca agatgctggt cgccacctct gaactcgaaa tggaacggat
     1681 gcgcgcgttg tgggaacgca cggcggcaaa cggtatcgag cgcgagtggt taaacgccga
     1741 tgaactgcgc gagcgcgaac cgaatatcac cgggcttggc ggtatttttg tgccgtccag
     1801 cggcattgtc agctaccgcg aagtcacggc ggcgatggca aaaattttcc aggccagagg
     1861 cggcgagatt atctataacg ccgaagtcag cggccttagt gagcataaaa gcggcgtggt
     1921 gatacgtacc cgtcagggca gcgactatga agcatcgacg ctgattagct gttccgggct
     1981 gatggctgac cggctggtga aaatgctcgg cctcgaaccg ggctttatta tctgcccgtt
     2041 ccgcggcgag tatttccgcc tggcgccgga gcataaccag attgttaacc atctgattta
     2101 ccccattccc gacccggcaa tgccgttttt gggcgtgcat ctcacccgca tgatcgacgg
     2161 cagcgtcacc gtcgggccaa acgcggtgct ggctttcaaa cgcgaaggct atcgcaagcg
     2221 cgacttctca tttagcgaca cgctggagat tttgggctcg tcggggattc gccgggtgct
     2281 gcaaaaccat ctacgctcag gactgggcga gatgaaaaac tcgctgtgca aaagcggcta
     2341 tctgcggctg gtgcaaaagt attgtccccg gctttcgtta agcgatctcc agccctggcc
     2401 cgccggtgtg cgggcgcagg cggtatcgcc ggacggcaag ctgattgacg attttctgtt
     2461 tgtcaccacc ccgcgcacga tccacacctg caatgcgccc tccccggcag cgacatcagc
     2521 aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg ctgttggcaa gccagagtaa
     2581 ccccggacgc acgctgcgag cggcacgtag tgtggatgcc ttacacgccg cgtttaatca
     2641 ataacctttg aaaacaggat gtagcgatga aactgaacga cagtaactta ttccgccagc
     2701 aggcgttgat taacggggaa tggctggacg ccaacaatgg tgaagtcatc gacgtcacca
     2761 atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat gggcgctgat gaaactcgcg
     2821 ccgctatcga cgccgccaac cgcgccctgc ccgtctggcg tgcgctcacc gccaaagaac
     2881 gcgccaacat tctgcgcaac tggttcaatt tgatgatgga gcatcaggac gatttagcgc
     2941 gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc gaaaggcgaa atcagctacg
     3001 ccgcctcttt tattgagtgg tttgccgaag aaggcaaacg catttatggc gacaccattc
     3061 ccggtcatca ggccgataag cgcctgattg tgatcaagca gccgattggc gttacagcgg
     3121 ccatcacgcc gtggaacttt ccggcggcaa tgattacccg caaagccggt ccggcgctgg
     3181 cggcaggctg caccatggtg ctgaagcccg ccagtcagac gccgttctct gcgctggcgc
     3241 tggcggagct ggcgatccgc gcgggcattc cggctggcgt gtttaacgtg gtcaccggtt
     3301 cggcgggcgc ggtgggtaat gaactgacca gcaacccgct ggtacgcaaa ctgtcgttta
     3361 ccggctcgac cgaaattggc cgccagttaa tggaacagtg cgcgaaagac atcaaaaaag
     3421 tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt tgacgatgcc gatctcgata
     3481 aagccgtgga aggcgcactg gcctcgaaat tccgcaacgc cgggcaaacc tgcgtctgcg
     3541 ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt tgccgaaaaa ttgcagcagg
     3601 cggtgagcaa actgcatatc ggcgacgggc tggataaagg cgtcaccatc gggccgctga
     3661 tcgatgaaaa agcggtagca aaagtggaag agcatattgc cgatgcgctg gaaaaaggcg
     3721 cgcgcgtagt ttgcggcggt aaagcacatg aacgcggcgg caacttcttc cagcccacca
     3781 ttctggtgga cgtgccggcc aacgccaaag tgtcgaaaga agagaccttc ggccccctcg
     3841 ccccgctgtt ccgttttaaa gatgaagccg atgtgatcgc gcaagccaat gacaccgagt
     3901 ttggccttgc cgcctatttc tatgcccgtg atttaagtcg cgtcttccgc gtgggcgagg
     3961 cgctggagta cggcatcgtc ggcatcaata ccgggattat ttccaatgaa gtggccccgt
     4021 tcggcggcat taaagcctcg ggtctgggtc gtgaaggttc gaagtatggc atcgaagatt
     4081 acttagaaat caaatatatg tgcatcggtc tttaactgga gaacgcgaat gagcagcaat
     4141 aaagagttaa tgcagcgccg cagtcaggca attcctcgtg gcgttgggca aattcacccc
     4201 attttcgctg atcgcgcgga aaactgccgg gtgtgggacg ttgaaggccg tgagtatctt
     4261 gatttcgcgg gcggcattgc ggtgctcaat accgggcacc tgcatccgaa agtggttgcc
     4321 gcggtggaag cgcagttgaa aaaactgtcg cacacctgct tccaggtgct ggcttacgag
     4381 ccgtatctgg agctgtgcga gattatgaat cagaaggtgc cgggcgattt tgccaagaaa
     4441 acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg cggtgaaaat cgcccgcgct
     4501 gccaccaaac gtagcggcac catcgctttt agcggcgcgt atcacgggcg cacgcattac
     4561 acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg gcatgggcct gatgccaggg
     4621 cacgtttatc gcgcgcttta tccttgccca ctgcacggca tcagtgaaga tgatgctatc
     4681 gccagtatcc accgaatttt taaaaatgat gctgcgccgg aagatatcgc cgccatcgtg
     4741 attgagccgg ttcagggcga aggcggtttc tacgccgcga cgcctgcgtt tatgcagcgt
     4801 ttacgcgcgc tgtgtgacga gcacgggatc atgctgattg ccgatgaagt gcagagcggc
     4861 gcggggcgta ccggcacgct gtttgcgatg gagcaaatgg gcgtggcacc agatctcacc
     4921 acctttgcga aatcgatcgc aggcggcttc ccactggcgg gcgtcaccgg gcgcgccgaa
     4981 gtgatggatg ccgtcgctcc aggcgggctg ggtggcacct atgccggtaa tccgattgcc
     5041 tgcgtggcgg cgctggaagt gttgaaggtg ttcgagcagg aaaatctgct gcagaaagcc
     5101 aacgatctgg ggcagaagtt gaaagatgga ttgttggcga tcgccgaaaa acaccctgag
     5161 atcggcgacg tacgcgggct gggggcgatg atcgccatcg agctgtttga agacggcgat
     5221 cacaacaagc cggacgccaa actcaccgcc gagatcgtgg ctcgcgcccg cgataaaggc
     5281 ctgattcttc tctcctgcgg cccgtattac aacgtgctgc gcatccttgt accgctcacc
     5341 attgaagacg ctcagatccg tcagggtctg gagatcatca gccagtgttt tgctgaggca
     5401 aagcagtagc gccgctccta tgccggaggc gacgctgcgc gtcttgtccg gcctacgggg
     5461 atccaggtcg gataaggcgt ttacgccgca tccgacaatc tgtacgtgaa caggaataaa
     5521 tctatgttgg ccgggtaagg cggagccgct ctccggcaaa aagaatcaat aacaattata
     5581 cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga
     5641 ttcaggatgg ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa gtcacgccat
     5701 gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc
     5761 gccatcgccg aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg
     5821 gttatgatta tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt
     5881 tccacctatg ccgataaagc tattggtcgt tgggcgggtt ataccatcgg ctggttgtac
     5941 tggtggtttt gggtactggt tatcccgctg gaagccaaca tcgccgccat gatcctgcac
     6001 tcatgggttc caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact
     6061 ggcagtaatt tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc
     6121 aaagtcatcg ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac
     6181 ccgtatgccg acgtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac
     6241 ggcttcggtg cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa
     6301 attgtcacca ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgctacc
     6361 aactcggtta tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtagtggcg
     6421 ttaataccgt ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa
     6481 ttgctcaata taccccatgc gaaattaatc atggactgcg tgatattact ttccgtaacc
     6541 agttgcctga actcggcgct gtataccgcg tcaaggatgc tctactcctt aagccgtcgc
     6601 ggtgatgcgc ccgcggtaat gggcaaaatc aaccgcagta aaaccccgta tgtggcggtg
     6661 ttactctcca ccggagcggc atttttaacg gtcgtggtga actattacgc acctgcgaaa
     6721 gtgtttaaat tcctgatcga cagctccggt gctatcgccc tgctggttta tttagtcatc
     6781 gccgtttcac agttgcggat gcgtaaaatt ctgcgagcag aaggaagcga aattcgcttg
     6841 cgcatgtggc tttacccgtg gctcacctgg ctggtaatcg gctttattac ctttgtgttg
     6901 gtggtgatgt tattccgccc ggcgcaacag ttagaagtga tctctaccgg cttattagcg
     6961 atagggatta tctgtaccgt gccgattatg gcgcgctgga aaaagctggt attgtggcaa
     7021 aaaacacccg ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt
     7081 ctctggatgg ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg
     7141 aaaaattacg aatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttacggg
     7201 aagctctttc gcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc
     7261 gggtggcgtc tatgtcagag caggagctgc tcgatatttt cgacgcccgc gccaatatgg
     7321 aagcgatgtt agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc
     7381 tcgcaaaagc gcatctgctg agtaagcttg aggcctgtga cgccagcgag aaaatgcttg
     7441 atgagtggga tctgcgtcat caggcgtttc atactgcaat tgtggcgggc tgtggttctc
     7501 actatttgct gcaaatgcgt gaacggttgt ttgatctggc ggcgcgttat cgatttatct
     7561 ggctgcggcg aacggtgctt tcggtggaaa tgctggagga taaacacgat cagcaccaga
     7621 ccctgactgc ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc
     7681 atttactgac gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc
     7741 ggaatacgca acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc
     7801 gcttcgaaga ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc
     7861 agagtgtcgc cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc
     7921 acctgatcat caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc
     7981 ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt tgccatcggc aatttgaatg
     8041 ttcactttat cggcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc
     8101 tgatcatctt tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc
     8161 acaaaattga acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag
     8221 tagaggaacg cggcgcgtta acgccgcggg gatgagcaca ggtttaatcg cgggtttgca
     8281 cccagaacgc gtgaatcaaa ccggggatat acccaagcag tgtcaacaga atattaataa
     8341 tgaacgccca accgaaccct ttaccgagca gcacgccgag cggcggcaga atgatggtga
     8401 tgacgattct ccagaaaccc atatgtactc cctataagaa aattacttat tgtttaaaaa
     8461 gagattttat ctcttaagcg tagtaatttt agcggaggct gccagtttca gcttacgctt
     8521 ttactctcat ttacgctgct gaggctggta tatgttgcat ttttagttta gagtttactt
     8581 aatttagaaa atacttaaat aactatgacc gaactcacgc aattacaggc cagtgctgaa
     8641 caggcagcgg ccttattgaa agcaatgagc caccctaaac ggttgctgat tctgtgcatg
     8701 cttagcggtt cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagcgcc
     8761 tctgcgacat cacagcatct cgctcgtatg cgggacgaag ggcttatcaa cagccaacgg
     8821 gatgcccaac gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc
     8881 ctgaaaaatg tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat
     8941 gatgcacaag aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa
     9001 tatcttcgtg aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca
     9061 ggtctcccgg ctaaattacg tcgcgatcaa attatattcc actgccaggc aggtaaacgc
     9121 accagtaata acgccgataa attagcagcg attgccgccc ccgcagaaat ctttttactc
     9181 gaagatggga ttgatggctg gaaaaaagca ggattaccag tagcggtaaa taaatctcaa
     9241 cccttgccgt taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt
     9301 atactgggtt ataccgtaaa tagcggtttc ttcttattaa gcggctttgt tggtgccggg
     9361 ttactgtttg caggaattag cggtttttgt gggatggcaa ggttgttaga taagatgccg
     9421 tggaaccaat gagcttgaga agcgacgccg gacgcgccct agcagcgaca tccggcctca
     9481 gtaattagat caggaaatcg tcgagagatt taccttctgc cagcgcctga gcgattggct
     9541 tcggtgtacg gccctgaccg gtccaggttt tagtttcacc gttaacatcg gtgaatttat
     9601 atttcgccgg acgcggctgg cgttttttac cagcacgtgg tgcagcagca gagctattac
     9661 ccaataactc ttccgggtta attccgtcag ctttcatcag ctccagccag gtgctaattt
     9721 tttcctggcg ctctgccagt tcacgctgct gctgttcttc ttcttcacgt ctttctttag
     9781 tgacaaccct gaatttttcg agcatttctt caagaacgtc aatggagaat tcgcgagcca
     9841 tcgcacggag ggtgcgaatg ttatttaaac tttgtaacat tacggacata ataaagaaaa
     9901 ccttttaacg ccaaaacaaa aaagtatttc gtcgcgtagt gtaagcgatc acttattata
     9961 tttccacaaa atttaatcta tttacagtcc tgcgcgagat tatttcagct gttattttct
    10021 g
//
LOCUS       DAZFDC010000150         9811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFDC010000150 DAZFDC010000000
VERSION     DAZFDC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.150"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..150)
                     /locus_tag="ACHJ48_004097"
     CDS             complement(<1..150)
                     /locus_tag="ACHJ48_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709544.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLN"
     gene            228..1322
                     /gene="ddlA"
                     /locus_tag="ACHJ48_004098"
     CDS             228..1322
                     /gene="ddlA"
                     /locus_tag="ACHJ48_004098"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO4709545.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1346..1558)
                     /gene="yaiZ"
                     /locus_tag="ACHJ48_004099"
     CDS             complement(1346..1558)
                     /gene="yaiZ"
                     /locus_tag="ACHJ48_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO4709546.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1818..2126
                     /gene="yaiY"
                     /locus_tag="ACHJ48_004100"
     CDS             1818..2126
                     /gene="yaiY"
                     /locus_tag="ACHJ48_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO4709547.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2185..3279)
                     /gene="yaiW"
                     /locus_tag="ACHJ48_004101"
     CDS             complement(2185..3279)
                     /gene="yaiW"
                     /locus_tag="ACHJ48_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO4709548.1"
                     /translation="MSRVNHLSSLSFLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSGIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3292..4512)
                     /gene="sbmA"
                     /locus_tag="ACHJ48_004102"
     CDS             complement(3292..4512)
                     /gene="sbmA"
                     /locus_tag="ACHJ48_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO4709549.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4864..6021
                     /gene="ampH"
                     /locus_tag="ACHJ48_004103"
     CDS             4864..6021
                     /gene="ampH"
                     /locus_tag="ACHJ48_004103"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO4709550.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6022..6645)
                     /gene="iprA"
                     /locus_tag="ACHJ48_004104"
     CDS             complement(6022..6645)
                     /gene="iprA"
                     /locus_tag="ACHJ48_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO4709551.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6733..9675)
                     /gene="ehaB"
                     /locus_tag="ACHJ48_004105"
     CDS             complement(6733..9675)
                     /gene="ehaB"
                     /locus_tag="ACHJ48_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO4709552.1"
                     /translation="MHSWKKKLIVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMITSECMTTDCADDRATGYVYDRLTLSVDNSTIDDNYEHYTYNGTYN
                     NAADTHVVDVYDMGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNS
                     SEVFNNTITVKDSTVTSGSWTDEGTTGWFGHTGNASNYSNTLTADDVAIAAIANPYAD
                     NAMQTTVTLDNSTLMGDVVFSSNFDENFFPQGANSYRDADGDVDTNGWDGTDRMDVTL
                     NNGSKWVGAAMSVHMVDEDGDGSYDGYAVGTEATATLLDIAANSLWPSSTVGVDNINT
                     QYDENGHIVGNEVYQSGLFNVTLNGGSEWDTTKSSLIDTLSINSGSQVNVADSRLISD
                     TVSLTGGSNLNIGEDGHVATNTLTIDNSTVKMSDDVSAGWGLEDAALYANTITVTNDG
                     LLDINVDQFDANPFQADTLNLTSTTDTNGNIHAGVFDIHSSDYVMDTDLVNDRTNDTT
                     KSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDN
                     ATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTD
                     YANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINY
                     DQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFA
                     NNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPY
                     GSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYV
                     YDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSA
                     NVGVKYTW"
BASE COUNT         2519 a         2435 c         2333 g         2524 t
ORIGIN      
        1 attaagagaa aaaaactcac cgcatggcca tttaagccag gaataaatta gcttcccgtt
       61 catcattttt gtgatcttgc gcacatcttt gagataacgc actttcttca ccagtacgtt
      121 gcacgctttg tcagcagaag attgcaacaa tctttcagaa ggccgcgaat cccccgtttt
      181 gtcgtggtaa tgtatgcact ttgtgttaga cgggataggt ttttaagatg gaaaaactgc
      241 gggtaggaat cgtttttggt ggtaaatcag cggaacatga agtgtctctg caatcggcaa
      301 aaaacattgt cgatgccatt gataaaagtc gcttcgacgt tgtgctgctg ggcattgata
      361 aacaagggca atggcacgtc agcgatgcca gcaattatct gctaaatgca gacgatcctg
      421 cccatattgc gttgcgccct tcggcgacca gcctcgccca ggtgccaggt aaacatgagc
      481 atcagcttat cgacgcgcaa aacggtcagc cgttgccgac ggtggatgtc attttcccga
      541 ttgtccacgg tacgctgggc gaagatggtt ccttgcaggg aatgctgcgg gtcgccaatt
      601 taccgtttgt aggttctgat gttctggctt cagcagcctg tatggataaa gatgtcacca
      661 aacgtctgct acgcgatgcc gggctgaaca ttgcgccatt tattaccctg acgcgcgcta
      721 atcgtcacaa catcagtttt gccgaagtgg agtctaaact ggggttacca ctgtttgtaa
      781 aaccggctaa tcagggctct tctgttggtg tcagcaaagt aaccagtgaa gaacaatacg
      841 caattgccgt cgatctggcg ttcgagttcg atcataaagt gatcgttgag caagggatca
      901 aaggtcgtga gatcgaatgc gcagttctgg gcaacgacaa tccgcaagcc agcacctgtg
      961 gcgagatcgt actcaccagc gatttctatg cctacgacac caagtacatt gacgaagatg
     1021 gcgcgaaagt ggtagttccg gcagccattg cgccagaaat caacgataag atccgggcga
     1081 ttgccgttca ggcttatcag acgttgggat gcgcaggcat ggcgcgtgta gacgtgtttt
     1141 taaccccaga gaacgaagtg gtgatcaacg agatcaacac actgcctggc ttcaccaaca
     1201 tcagtatgta tccgaagctg tggcaagcca gcggtctggg ttacaccgat ctgatcacac
     1261 gtttgattga gctggcgctg gagcgtcacg ctgcggataa cgcattgaaa accacaatgt
     1321 aatattttaa cgcccgataa tattcttatt gggcgttttc agtttcttca tcgcgacgac
     1381 gaataatcaa ccccgccagc cagaaactaa tcacccacgt caccagaccg acggcatagg
     1441 tctgccaacc ttttgcttca aatcccagta accccaccac gccattaaga atgaatataa
     1501 ggccaatggc gaacgcatag tagtgccagt cacggcggat ttttacaggc agattcatgg
     1561 ctgctccgtt aacgaaaaac aacatccttg catattctgc aacaaccgtc tgtggtgtag
     1621 acaacatttt cgcaattgtt aataaattta acttaaagtt gctattttgt gactcgcaac
     1681 gaagattcac gcttcgggaa tattcccaac atcaatttac catgattctg atattgacaa
     1741 tctaaatgac ctgtcagact tgccataagc cgctggtatc acaccagaag agaagcagat
     1801 gacttccgga ggtttttatg gctgatttca ccctgtcaaa atcgctgttt agcggaaaat
     1861 atcgcaatgc ctcttcaacg cctggcaaca ttgcctatgc gttgtttgtg ctgttttgct
     1921 tttgggctgg ggcgcaattg ctgaacctgt tagtgcatgc gcccggcgtc tatgagcgtt
     1981 taatgcaggt ccaggaaaca ggtcgcccac gggtggaaat tggtttaggt gtcggcacca
     2041 ttttcgggct gatcccgttt ttagtaggct gcctcatttt tgcagtggtg gcgctatggc
     2101 tgcactggcg acatcgccgc cagtaagatt tacgttgcca gatacggcct aaaacgcctt
     2161 atctggcatg acattcctgt ttgttcactg tttcatacaa cgcgcccgcc gttcgtctac
     2221 gcgcttcgca aaccaggccg tagtcaggtt gcgggtgatt ttcgggcttt ccagttgaat
     2281 gccaggcaac atttcgcggg ggagtgattt acccgttttc gcttcggcaa gttgataaac
     2341 tttcttgtac agcgccgtct cctcaaaaga gaagctgtca cctttctcta gctgacgacg
     2401 aatttcgctg tcgctcatcc ccagttttgc cgccagtttg cgcgttgcca gttcggtttt
     2461 cccgggttct ttactgtcgt aacgaattaa atcaccgtcc agtgccagct tcacgccgct
     2521 ggccttactg accgcgttct gaaacgcggc attacggctg gcgtaccagc cagcgttaaa
     2581 atcagcaaaa cggtatatcg gtgcgctata actggcggga taattcagta aatggtaagt
     2641 accaaaccac aacccgccgc ggcgactaaa gacttcctga cggactgtac cgtccatttt
     2701 ccacggatac cctttggtat gctgttcggc aaaagcaatg ctgacctgca tcggcccacc
     2761 ggtgcgcacc ggattaagcg aaccaaacaa cgtctgcccc attggcacca tgctgatcag
     2821 atcgtcaaaa atcccgctca attgcttttc tgtacgcacc gaatctaacc gttcgctata
     2881 acttttgccg tttggcgatt tgatttttag cgcagtatga accagaaaag caggaatgtg
     2941 catccgttca gcacgacggt caatctcttg ccaggcgatc ttgcttaagc ccggaacggc
     3001 cggatctgcc tgataatttg actcctgctg cgccaccgcc agcaccgagc agacgttttc
     3061 cagcgtcggt gccagcccct gactttcaaa ggtggtggcg agatctttcg cccaggcatc
     3121 gcggtctttc acgctcgcag gcatcttctg ccgcaccacg ctagccacat ctatcgcttt
     3181 ttcgcccttt ttcaacggct gtggcgcctg actgctacac cccgctaaaa ccagaaccgc
     3241 caaaaatgaa agcgaagaca gatgattgac tcgcgacatt acttctcctt tttagctcaa
     3301 ggtatgggtt acttcctgaa ttttgtcacc atccagctca tgttcaaagc tgcgcagacg
     3361 tttgtagata gacatcaact caaccagtgt ggtccatgag ttaatcaggt actggaaagc
     3421 accgcgaacc tgaccaaaaa cgttggtaat ctgcgtcatc aggccgagcg taatcgtacc
     3481 ggcaacaatt gacggaaaca gcaagaacaa accgaaaacg ttatcgacct gcaaatagag
     3541 aatgcgagcg atgttgaaat acatatagtg aaaatagagg cggaaatagt ttttccgtac
     3601 ggcgctaaac agctcgcgta ccgttggcgg cgtcgcgcgc gtggcatcgt cttcaccata
     3661 aaccagctct ttacggtagg cagcctctac acgctggttt ttaaactcca gccccggcag
     3721 tttgatccct accactgcca gcaatccggt ccccatcagc gaccagacga tagcggcaat
     3781 caccagacca tacggaatgt gcccgataat cggcagctcc ggcacatgcg cggagagcgt
     3841 taccaacacc ggcaggaagg cgatcaacgt catgatggcg ttgataaaac tgacacccat
     3901 attctccagc gttgaagcaa aacgcatggt gtcttcctgc acacgctgtg cggcgccttc
     3961 gatatgacgc agttgttgcc agttcgccat gtaatattcg ttcatcgccg tacgccagcg
     4021 gaacacgtag tgactgacaa agaagttatt cagcacactg atcactaccg cgatcagcgc
     4081 aatccccaga aagacgccca cttcgcggta aaattgttcg atagtgactt tatgcggcga
     4141 acttagcgcg gtttgaatca gatcatagaa cggtgcatac caggcgttga cggcaacccc
     4201 aacttccacc aaaaaccagg tgacgaagat gatcagtgca gtaccgagta ttgaccagta
     4261 ttgccaacgg tgcgggctgt agataaacca gaacaatgca aaaagtccta cgcaaacaat
     4321 gtagtaagcg taaaaaatca ggaaatccaa cgaccagaaa cgcgcggcgc taatcgggat
     4381 ctgcccggaa gcgccggtga tacgcgccac ccagtccccc ccaccggctt gccagaagat
     4441 aacggcaatc aatgcccaaa caaaggccga gagaaaaaac gttcccggct ttgggaaaaa
     4501 agacttaaac atatgcactc ctgctaactt cttatcgttt taacaactgc tgtgtattaa
     4561 ccgcatgacc gctacaaagc gtcagccaaa tgaaaccgcc cattgtgact catagcaagg
     4621 cgcttagttc gccagcctgg ccataagaat tgtttcgacg gatttcacaa accaaagatt
     4681 gtttacataa gcggattaac acgcgcagga tcacaaatgc aggcattggt gagcaggaaa
     4741 aagatcgtgt ttacgcgccg ttacgttttt aacactatta ttgcactaat tcgccctttg
     4801 caatccatca atgagtagta taaatacgct cagttacctt cattcaattt atggacacca
     4861 ccgttgaaac gtagtctgct tttttctgcc gtgctgtgtg cggcgtcatt gacctccgtc
     4921 catgcggcgc agccgatcac tgaaccggag tttgcctctg atattgtcga tcgttatgcc
     4981 gatcatattt tttacggcag cggtgccacg gggatggcgc tggttgttat cgacggtaac
     5041 cagcgcgtct ttcgcagtta tggcgaaacg cgacctggta ataacgttcg cccgcagctg
     5101 gattcggtcg tgcgtattgc ttccctcacc aagctaatga ccagtgagat gctggtgaaa
     5161 ttgctcgacc aggggaccgt gaagttaaac gatccgctaa gtaaatatgc tccgccaggc
     5221 gcacgcgtgc caacctacaa tgggacgccc attacgctgg tcaatctggc aacccatacc
     5281 agcgccctgc cccgtgaaca gcccggtggc gcggcacatc gtccggtgtt tgtctggcca
     5341 acgcgcgagc aacgctggaa atacctttct acggcgaagc tgaaagccgc gccaggcagt
     5401 caggcggcgt actctaacct tgcgttcgac ctgctggcag atgcactggc gaatgcgtcc
     5461 ggcaagcctt atacccagtt gtttgaagag cagattaccc gtccattggg gatgaaagac
     5521 accacctaca ctccctcacc ggatcagtgc cgccgtttaa tggttgctga gcgcggtgcc
     5581 agtccgtgca ataacacgct ggcggcaatt ggcagcggcg gcgtctattc cacgcctggc
     5641 gatatgatgc gctggatgca gcagtatttg tcatctgatt tttatcagcg cagtaaccag
     5701 gcagatcgca tgcagacgtt gatttatcag cgcgcgcagt ttacgaaagt gattggcatg
     5761 gatgtccccg gcaaagcgga tgcgcttggt ttaggctggg tatatatggc cccgaaagag
     5821 ggtcgtccgg ggattattca gaagacaggt ggtggtggcg gtttcattac ctatatggcg
     5881 atgatcccgc agaagaatat tggcgcgttt gtggtggtca ctcgttcgcc gttgacgcgc
     5941 tttaaaaata tgagcgatgg catcaatgat ctggtgaccg agctaagcgg gaataaaccg
     6001 ttggttatcc ccgcatcctg attaatactc tgaaggcaaa cggttgatag cgaccagttt
     6061 gcctttattc atttcgatat agccgccttt acgcaaagca gcgagaactt ctgcgacgac
     6121 agaacgcgat atgcgtgtac gttgatggat ataattcatc acaccaatac gtgatcgcaa
     6181 ttcttcattc cagtcaatca ttgataataa tgttgcgcgg atttgttcgt aggaattatg
     6241 cccaatgagc tgcacgtcgc gtaattccag aattctattt tgccaggcta accagtaaaa
     6301 agcgtctcgc cagagttgat tttgttcaat aagcgtaatg gtttgtttgg ctggtagatg
     6361 atatcccgta caatttcctt ctgatattaa tttgtatggt atatcgtttt tcattaaacc
     6421 atcagccagc cccataatat aaggagcctg ggtaataccg ataagtacgt tttcttctct
     6481 acgcagagag ataactccct ctaaaataac gaaagtatct tcgttattga catcactgga
     6541 aaatataact tgcttttcat tattaaactc gaagcgcgta ccgtatctgg acaaacattt
     6601 atcgagctta ccaaattcct gaagaggttt aactacagat aacatttgcg cgtcctttgc
     6661 agtaatgccc gtcaaatcct tgacgggcat tatttagatt aaattaccag tatttcttcg
     6721 gagtgaagaa tattaccagg tatatttaac acccacgttc gcggaccagt cttgatctac
     6781 gtcaccacca ccgaggtagt tagcatcggt ataggcgctg aagttcttgg tgaagctgaa
     6841 ctgagtaccc agaccaacac gtaccgcaga cccttcagta ccgttatcga tggaatcacc
     6901 gttcacatcg ttatcgttgt tagagtcgtc gtagacgtaa gccagtttga agtacggagt
     6961 cagagcctga tcttcgctgt aggtgaaggt ataacctgca tctacaccca gttcataacg
     7021 catgctgtcg taagactgac cgtcaacttt catgtcgttg ctcagctggt agtcatcacc
     7081 agactggaac agaccagaaa tgctgccgta aggagtcacg taaccagcat cacccagttt
     7141 gaagtcgtaa ccggctttca aaccgaagcc ccaagcgtcg gagttggtgc taccgtcaac
     7201 gtaagtaccg ttgctcatgg ttgcagacag gtcgttgttg aagtgagagt agctcaagct
     7261 accatcaaca aagacgttgt tcgcgaagtg agcagaagag tagatgtagg cagtctggct
     7321 gtcttgatcc acctgaccag aacggtcatt catgtcacct ttagcgaagc ctgcagccgc
     7381 accgacgatc cacttagcgt tgttaccgtc aattttggta tcaacaccga ccatgatgcc
     7441 gttaacatcc tgatcatagt tgatggtgcc gttgtcgccg ttgaagttac caccgaagta
     7501 gcttacccat gcgccgccgt tatcagccag gccatgacga gagttggtca aacgagtacc
     7561 aacggtgtct tgttccaggt tccagatatt ggtgttcgca gatgggatgc tcagcgccat
     7621 gttagcgtag tcggtcaact ccatctgttg cagaacaacg gtgttaccgc gctgttcagc
     7681 ctgataggtg tatgcaccca ggtcagcttt gttagcagca gagaaggtcg cgttgctgtt
     7741 tttgtcgttg acgtagatca gctcgttgcc gttgtagtca gcgatagaac cagcaccagt
     7801 agcgttgtcg atacgaactt tgtagttacc ggtcgcggct gcaacatggt caccgttgtt
     7861 atcaacttcg ttctgaccag cttcgataga agcagtgttg tcgttatcgc cgttaccgtt
     7921 aatagtcagg tgaccatcag agttcattgc gattaagcca taaccgtagt ttgacttggt
     7981 agtatcgttg gtacgatcgt tgaccagatc ggtatccatt acgtaatcac tgctatggat
     8041 atcgaataca ccagcgtgaa tgttgccgtt agtatcagtg gtactggtca gattcagggt
     8101 atcggcctgg aacgggttag catcgaactg atcaacgtta atatccaaca gaccgtcgtt
     8161 agttacggtg atggtatttg cgtacagtgc agcatcttct aaaccccagc ccgcagaaac
     8221 atcatcagac attttaacgg tactattgtc gatggtcagg gtattagtcg ctacatgacc
     8281 gtcttcaccg atgttcaggt tagaaccgcc agtcagagag acagtgtcag agatcagacg
     8341 agagtctgca acattaactt gggaaccgct gttaatactt aaagtatcaa tcagagaaga
     8401 ttttgttgta tcccactctg aaccaccgtt caaagtcaca ttaaacaaac cgctctggta
     8461 aacttcgttt cctacgatat ggccattttc gtcatattga gtattgatgt tatcaacacc
     8521 gacagttgat gaaggccaca ggctgttagc tgcaatatcg agcagagttg cagttgcttc
     8581 agtaccaaca gcatatccgt cgtaagaacc atcaccatct tcatcaacca tatgaacaga
     8641 cattgcagcg ccaacccact tgctgccgtt gttcagagtc acatccatac ggtctgtgcc
     8701 atcccaaccg ttggtatcta catcaccatc agcatcgcga tagctgttag caccttgcgg
     8761 gaagaagttt tcatcgaaat tactggagaa aacaacatca cccatcagtg ttgagttgtc
     8821 taaagttact gtagtctgca tcgcattatc agcatacgga tttgcgattg cggcaattgc
     8881 aacatcgtct gcagtcagcg tgttgctata gttgctggca ttaccagtat ggccaaacca
     8941 accagtagta ccttcatcag tccatgaacc agaggtcaca gtagaatctt taacagtaat
     9001 agtgttatta aacacttcgc tgctgtttac gccagtgctg actgtgttgt cgtcaatatc
     9061 ttcccactca taaccctgag tcagcgtaat acctgctaca tgagagttat tagtgatgga
     9121 cagatcaact tcctgatcca gtgtaatagc agtacccata tcgtaaacat ctacaacatg
     9181 agtgtcagcg gcattattat aggtaccgtt gtaagtataa tgctcgtagt tgtcatcgat
     9241 cgttgaatta tcaacgctca gtgtcagacg atcataaaca taaccagtag cacggtcatc
     9301 agcacaatca gtagtcatgc actcagaggt aatcataccg tgaatagtgc tgtttttgat
     9361 tgtcagacta ttggcgttgg tattggtaga aataccgtcg tccagatagt aggtagaaat
     9421 cacgccgtta acggtagcgt tattaatgac cgggtagata tcgccgttgt aatagctatc
     9481 agcggcatag ttgttccagc ctacataacc atcatagtaa acgtcatcag cataggtggc
     9541 gtcgttgtag tgatggaaag tattgtaagt ttgaccagaa atatcgttgg tcgctgcatt
     9601 agcctgagag gtgatagcca gagtgcaagc caatgctaat tgtgatacta taagtttctt
     9661 tttccaggag tgcatttgtc atccctcctc agggacgtaa taagttgatc catcaattat
     9721 caatgcatag aaaacatctt atgtttcgcg ttgacgatta tgcggcgtta tagaggaatg
     9781 gttcaattat tctttcccct aagtccgatt g
//
LOCUS       DAZFDC010000151         9721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFDC010000151 DAZFDC010000000
VERSION     DAZFDC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.151"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            224..643
                     /locus_tag="ACHJ48_004106"
     CDS             224..643
                     /locus_tag="ACHJ48_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO4709553.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            643..1911
                     /gene="umuC"
                     /locus_tag="ACHJ48_004107"
     CDS             643..1911
                     /gene="umuC"
                     /locus_tag="ACHJ48_004107"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO4709554.1"
                     /translation="MFALCDVNAFYASCETVFRPELWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1957..2487)
                     /gene="dsbB"
                     /locus_tag="ACHJ48_004108"
     CDS             complement(1957..2487)
                     /gene="dsbB"
                     /locus_tag="ACHJ48_004108"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO4709555.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2633..4174)
                     /gene="nhaB"
                     /locus_tag="ACHJ48_004109"
     CDS             complement(2633..4174)
                     /gene="nhaB"
                     /locus_tag="ACHJ48_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO4709556.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4396..5115
                     /gene="fadR"
                     /locus_tag="ACHJ48_004110"
     CDS             4396..5115
                     /gene="fadR"
                     /locus_tag="ACHJ48_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO4709557.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5167..6699)
                     /locus_tag="ACHJ48_004111"
     CDS             complement(5167..6699)
                     /locus_tag="ACHJ48_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO4709558.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7062..8360
                     /gene="dadA"
                     /locus_tag="ACHJ48_004112"
     CDS             7062..8360
                     /gene="dadA"
                     /locus_tag="ACHJ48_004112"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO4709559.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8370..9440
                     /gene="dadX"
                     /locus_tag="ACHJ48_004113"
     CDS             8370..9440
                     /gene="dadX"
                     /locus_tag="ACHJ48_004113"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO4709560.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEMYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
BASE COUNT         2419 a         2397 c         2605 g         2300 t
ORIGIN      
        1 aatgtcatta tggcgaatgc ttctattcta ttttttagcc gggtgatatt tttcatttct
       61 gctggatgag cgtcgtcgcc agaaggccgc gtgagcacag gataagagaa cgaaaaatca
      121 gcagtctatg cagcgacaaa tattgatagc ctgaatcagt attgatctgc tggcaagaac
      181 agactactgt atataaaaac agtataactt caggcagatt attatgttgt ttatcaagcc
      241 tgcggatctc cgcgaaattg tgacttttcc gctatttagc gatcttgttc agtgtggctt
      301 tccttcaccg gccgcagatt acgttgaaca gcgcatcgat ctgaatcaac tgttgatcca
      361 gcatcccagc gcgacttact tcgtcaaagc aagtggtgat tctatgattg atggtggaat
      421 tagtgacggt gatttactga ttgtcgatag cgctattacc gccagccatg gtgatattgt
      481 catcgctgct gttgacggcg agtttacggt gaaaaaattg caactacgcc cgacggtaca
      541 gcttattccc atgaacagcg cgtactcgcc cattaccatc agtagtgaag atacgctgga
      601 tgtctttggt gtggtgatcc acgtcgttaa ggcgatgcgc tgatgtttgc cctctgtgat
      661 gtaaacgcgt tttatgccag ctgtgagacg gtgtttcgcc ctgagttatg gggtaaaccg
      721 gtggttgtgc tatcgaataa tgacggttgc gttatcgccc gaaacgctga ggcaaaggcg
      781 cttggcgtta aaatgggcga tccctggttc aaacaaaaag atctgtttcg tcgctgtggc
      841 gtggtttgct ttagcagcaa ttatgagctt tacgcagaca tgagcaatcg ggtgatgtcg
      901 acgctggaag aactatcgcc ccgcgtcgag atttacagta ttgatgaggc attctgcgat
      961 ctgacaggtg tgcgtaattg tcgcgatctg actgactttg gcagagaaat tcgcgcaacg
     1021 gtgctacaac gtacccatct tactgttggt gtggggatcg cccagaccaa aacgctggct
     1081 aagcttgcca atcatgcggc aaaaaaatgg cagcggcaga cgggtggggt ggtggattta
     1141 tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga cgtctggggg
     1201 attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt tctcgatttg
     1261 gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga aagaacggtg
     1321 cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac aaagcaggaa
     1381 attatctgtt cccgctcgtt tggtgaacgc atcacggatt atacgtcgat gcggcaggcc
     1441 atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca atattgtcgg
     1501 tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta ttacggcaat
     1561 agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat taacgccgct
     1621 acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc gggcgtgatg
     1681 ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga caacgctccg
     1741 cgccccggga gtgagcaatt gatggcggta atggatacac tgaatgctaa agagggcaga
     1801 ggaacactct attttgccgg gcaggggatc cagcaacaat ggcagatgaa gcgagccatg
     1861 ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata aacacagcgg
     1921 caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca ggtcacgttt
     1981 tttcgcttta aacggctggg aaatcaccac cagcaccgcg acaatcaggt aagcgataaa
     2041 aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac gctcggcgca
     2101 atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc attccgggaa
     2161 acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca tggtgtgctc
     2221 gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg ctacataacg
     2281 cagtggcgtt tttggggcga tcgcgccaat cagcgcagca cccagaacgc cgaataatgc
     2341 gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct ggaaccacag
     2401 cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc cccggccttg
     2461 tgaacattgg ttcaaaaatc gtaacataat catttccctg caatatgcat agagtgcgca
     2521 gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac gcaattggac
     2581 aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt tatcaaagcg
     2641 ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta aactcgacgc
     2701 agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac accatgcggc
     2761 cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaacagg aacgcagcct
     2821 gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg accgccagca
     2881 gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct tcgttgatat
     2941 aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg aaaatataga
     3001 acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc ggcgaaaaca
     3061 gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt gcggtgaatg
     3121 gcagagattc ggtgaaggct ttaccgatag catgttcatc ggtgacaccg gtcaatgatg
     3181 ttgccagaat aatgactgac aaaccaatca agccaacttc cgccagatgc aacgccagcg
     3241 cagtcaccag ccagacgcca ataatcgcct ggacaatcag acggatttta tcctgacggg
     3301 tacgctggtg gcggctttga tcgtcaaact gttgcaacac ttcgcggact ttctccggca
     3361 gcgtttcacc gtagccaaac caacgcagct tctctaccag caggcaggtt aacaggccac
     3421 aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca aaatgccagc
     3481 cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatt acgccgccta
     3541 atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg aactgttcca
     3601 gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggtg tcttcggtac
     3661 gggaagaggc tacgcgatga taaataccat aaaaaccgac ggcaacgctg atcaccaccg
     3721 ccacgacggt taaggcatcg aggaacgcgg agaggaacgc tgccgctacg caaaaagaga
     3781 gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg aacagcaata
     3841 gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag acttcaagat
     3901 ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat accgcttcga
     3961 tagccaacag gccaccgggg agcagcgggt agcatttcag ggccatcgcc agagtgaaaa
     4021 taaattccgc taccagcaac cagccagcga cgaaagggct gatgaggaaa attaacggat
     4081 ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg cccaaaaaat
     4141 tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc ttacaaataa
     4201 tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt gaggcaagtc
     4261 ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt ttcttctttt
     4321 tgtctgctat cagcgtagtt agccctctgg tatgatgagt ccaactttgt tttgctgtgt
     4381 tatggaaatc tcactatggt cattaaggcg caaagcccgg cgggtttcgc ggaagagtac
     4441 attattgaaa gtatctggaa taaccgcttc cctcccggga ctattttgcc cgcagaacgt
     4501 gaactttcag aattaattgg cgtaacgcgt actacgttac gtgaagtgtt acagcgtctg
     4561 gcacgagatg gctggttgac cattcaacat ggcaagccga cgaaggtgaa taatttctgg
     4621 gaaacctctg gtttaaatat ccttgaaaca ctggcgcgac tggatcacga aagtgtgccg
     4681 cagcttattg ataatttgct gtcggtgcgt accaatattt caactatttt tattcgcacc
     4741 gcgtttcgtc agcatcccga taaagcgcag gaagtgctgg ctaccgctaa tgaagtggcc
     4801 gatcacgccg atgcctttgc cgagctggat tacaacatat tccgcggcct ggcgtttgct
     4861 tccggcaacc cgatttacgg tctgatcctt aacgggatga agggactgta tacgcgcatt
     4921 ggtcgtcact atttcgccaa tccggaagcg cgcagtctgg cgctgggctt ctaccacaaa
     4981 ctgtcggcgt tgtgcagtga aggcgcgcac gatcaggtgt acgaaacagt gcgtcgctat
     5041 gggcatgaga gtggcgagat ttggcaccgg atgcagaaaa atctgccggg tgatttagcc
     5101 attcaggggc gataatccct tccgtttaaa aaacaaaccc ctcaaacgag gggttttttg
     5161 ttgtttttac agatttccca ttctcggcgg gcaacgttcc agcaactcga cgctgccgtc
     5221 ttcgttttgc tgttccagca tcacatcaaa tccccacagg cgatgcacat gcttcagtac
     5281 ttctttgcgc ccccgatcca gcggtgcgcg attatgtgga atgtaacgca gcgtcagcga
     5341 acggtcgccg cgcaaatcca cgttccagat ctgaatattc ggctccagat tacttaagtt
     5401 atattgcgac gatagccggt tacggatctc ccgataacct tcttcattat gaatagcgga
     5461 aatctccaga taattatgcc gatcatcatc cagcacggtg aagaagcgga aatcacgcat
     5521 cactttcggc gacaggaact ggctgataaa actctcatct ttgaaatcac gcatcgcgaa
     5581 atgcagcgtt tccagccagt cggaaccggc gatatccggg aaccagtatt tgtcttcttc
     5641 cgttggcgac tgacaaatcc gtttaatatc ctggaacatg gcgaacccga gggcatacgg
     5701 gttgatgccg ctgtaccacg ggctgttata ggggggctgg aagaccacat tggtgtggct
     5761 gtgcaaaaac tccagcataa aacgttccgt tactttccct tcatcataca gatggttaag
     5821 gatggtgtag tgccagaagg tggcccagcc ttcgttcatc acctgagtct gtttttgcgg
     5881 ataaaaatac tggctcacct tacgcacaat acgcaggatt tcacgctgcc atgattccag
     5941 cagtggcgca tttttctcca taaaatagag caggttttct tgtggttcgg acggataacg
     6001 gcgcgcttca gcaaccgttt tctcttcctc gcgcttcggc agggtacgcc agagcatatt
     6061 gacctgactt tgcaaatact cttcgcgact tttctgccgg gctttctctt cttgcagcga
     6121 gattttttgc gggcgtttgt accggtccac gccgtagttc atcagcgcgt ggcacgagtc
     6181 cagaagccgt tctacttcat caacgccata acgttcttcg cactcggtaa tatatttacg
     6241 ggcaaaaatc agataatcga caatcgaact ggcgtcggtc cagctacgga ataagtaatt
     6301 gtttttgaag aaagagttat gcccatagca ggcatgagcc atcaccagcg cttgcatggt
     6361 aatggtgttc tcttccatca ggtaagcgat acacgggtta gagttaatga cgatttcata
     6421 ggccagtcct tgctgaccgt gcttatacag ccgttcagtc tcgataaact ttttaccgaa
     6481 tgaccagtgc ggatagttaa ttggcatgcc gacgctggag taggcatcca tcatctgttc
     6541 tgaggttatc acttcaatct ggtgcgggta ggtatccagc cggtagagtt tcgccacccg
     6601 gtctatctct gccagataaa catccagcag gtcgaacgtc cagtcgggtc catcgctcaa
     6661 acgtgtggtg tccttattca tagaatcgat cgtcgccata cgcgcacctc attgttgtcg
     6721 gcgctctctg tgtggagcac ctcattgttg tcggcgctct ctgtgtggag cacctcattt
     6781 caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt tgcgatttct
     6841 tattatcaga gtgccactaa tccgtttctg aacggaattt tatgctggat aaaaagggcg
     6901 ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg tgagccagct
     6961 caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag atgtaattag
     7021 attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt gtcatactgg
     7081 gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga catgaggtca
     7141 ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat gccgggcaaa
     7201 tctcccccgg atatgctgca ccgtgggcgg caccaggtgt gcctttaaaa gcgattaaat
     7261 ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag ttccagttga
     7321 aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa aacaaagggc
     7381 ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc gccgaaacca
     7441 atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc gaacaacagt
     7501 atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg tatcagctgc
     7561 tggaatccag ccgcctggcg gaagtggagc ccgcgctggc agaagtagcg cacaaactga
     7621 cgggtggtct gcaattgccc aatgatgaaa ccggagactg ccagctgttt acccagaatc
     7681 tggcgcggat ggcggagcag gcgggggtta aattccgctt taatacgccc gttgaccaac
     7741 tgctttgcga cggcgagcaa atctacggcg tgaagtgtgg cgatgaagtg attaaggccg
     7801 atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc attgttgata
     7861 ttccggtgta tccgctgaaa ggctactcgc tgaccattcc aattgcgcag gaagatggtg
     7921 cgccggtatc caccattctt gatgaaacct acaaaatcgc cattacccgt ttcgataacc
     7981 gcattcgtgt tggcggaatg gcggaaattg ttggttttaa taccgagctg ttgcaaccgc
     8041 gtcgtgaaac gctggagatg gtggttcgcg atctctatcc acgcggcggt catgtcgagc
     8101 aggcgacttt ctggacgggt ctgcgcccga tgacgccaga cggcacgccg gttgtcgggc
     8161 gtacacgctt taaaaatctg tggctaaata ccggtcacgg cacgctcggc tggacgatgg
     8221 cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca gcgatcccat
     8281 atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt ccgggccatt
     8341 tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc agcctcgatc
     8401 tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac gcgcgcgtct
     8461 ggtcggtggt aaaagcgaac gcttacgggc acggtattga gcgtatctgg agcgcgctcg
     8521 gggctaccga tggctttgca ttacttaacc tggaagaagc aataacgtta cgtgagcgcg
     8581 gctggaaagg accgatcctg atgctggaag gatttttcca tgctcaggat ctggaaatgt
     8641 atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa gcactgcaaa
     8701 atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg atgaatcggt
     8761 tgggcttcca gcccgatcgc gtgcttaccg tctggcagca gttgcgggcg atggcgaatg
     8821 ttggcgaaat gaccctgatg tcgcattttg ccgaggcgga acatcctgat ggaatttccg
     8881 gcgcgatggc gcgtattgag caggcggcgg aagggctgga gtgtcggcgt tcgttgtcca
     8941 attcggcggc gactctgtgg cacccagaag cgcattttga ctgggttcgg cctggcatta
     9001 ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatact ggattacgtc
     9061 cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg ggcgagcgtg
     9121 tgggctacgg cggtcgttat actgcgcgcg atgagcagcg aatcggcatt gttgccgcag
     9181 gatacgccga cggttatccg cgccacgcgc ctaccggtac ccctgtttta gtggacggcg
     9241 tgcgcaccat gacggtgggg acagtctcga tggatatgct ggcggtcgat ttaacgcctt
     9301 gcccgcaggc gggtattggt acgccagttg agctgtgggg caaggagatc aaaattgatg
     9361 atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcgctg gcgttacgcg
     9421 tcccggttgt gacggtgtaa cttgttgtaa gtcggatcgg aggcaacgtc ttctggttgc
     9481 aaaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac agagccattg
     9541 cccatgatag tgcccattaa aaggatggac actatttccc cggaacctga actcaccgca
     9601 caggcgttct acataaaacg cttacgcttc attgttgact cgaactcgac ttcagataaa
     9661 tcacgcttca cccttgatgc agattcgggt tcatgttcag aatgttgatt aacaggctgc
     9721 g
//
LOCUS       DAZFDC010000152         9338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFDC010000152 DAZFDC010000000
VERSION     DAZFDC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.152"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            12..86
                     /gene="tnaC"
                     /locus_tag="ACHJ48_004114"
     CDS             12..86
                     /gene="tnaC"
                     /locus_tag="ACHJ48_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO4709561.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            307..1722
                     /gene="tnaA"
                     /locus_tag="ACHJ48_004115"
     CDS             307..1722
                     /gene="tnaA"
                     /locus_tag="ACHJ48_004115"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO4709562.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENASNIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1813..3060
                     /gene="tnaB"
                     /locus_tag="ACHJ48_004116"
     CDS             1813..3060
                     /gene="tnaB"
                     /locus_tag="ACHJ48_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO4709563.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3192..4367
                     /gene="mdtL"
                     /locus_tag="ACHJ48_004117"
     CDS             3192..4367
                     /gene="mdtL"
                     /locus_tag="ACHJ48_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO4709564.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIITSVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLTVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGISAWNMLIGILIACSIVSLLLIMFVAPGRPVTAHEEIHHHA"
     gene            4342..5301
                     /gene="yidZ"
                     /locus_tag="ACHJ48_004118"
     CDS             4342..5301
                     /gene="yidZ"
                     /locus_tag="ACHJ48_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO4709565.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5458..6207
                     /gene="yieE"
                     /locus_tag="ACHJ48_004119"
     CDS             5458..6207
                     /gene="yieE"
                     /locus_tag="ACHJ48_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO4709566.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWVSKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            6229..6795
                     /gene="yieF"
                     /locus_tag="ACHJ48_004120"
                     /gene_synonym="chrR"
     CDS             6229..6795
                     /gene="yieF"
                     /locus_tag="ACHJ48_004120"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO4709567.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6849..8186)
                     /gene="adeP"
                     /locus_tag="ACHJ48_004121"
     CDS             complement(6849..8186)
                     /gene="adeP"
                     /locus_tag="ACHJ48_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO4709568.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRIRDLSPCVIIVALLFILKIVFIDAH"
     gene            8352..9017
                     /gene="yieH"
                     /locus_tag="ACHJ48_004122"
     CDS             8352..9017
                     /gene="yieH"
                     /locus_tag="ACHJ48_004122"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO4709569.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWN
                     ITR"
     gene            complement(9154..>9338)
                     /locus_tag="ACHJ48_004123"
     CDS             complement(9154..>9338)
                     /locus_tag="ACHJ48_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709570.1"
                     /translation="NALNAKIVDCDDKIRSKLTIVRNNRLENQYKDDKNISDAMRNIL
                     DWFERYPDIVQNITQA"
BASE COUNT         2302 a         2325 c         2317 g         2394 t
ORIGIN      
        1 ataagtgcat tatgaatatc ttacatatat gtgtgacctc aaaatggttc aatattgaca
       61 acaagattgt cgatcaccgc ccttgatttg cccttctgta gccatcacca gagccaaacc
      121 gattagattc aatgtgatct atttgtttgc tatatcttaa atttgccttt tgcaaaggcc
      181 atctctcgtt tatttacttg tgttagtaaa tgatggtgct tgcatatata catggcgaat
      241 taatggatat tgcagatgca atattcacag ggatcactgt aattaaaata aatgaaggat
      301 tatgtaatgg aaaactttaa acatctccct gaaccgttcc gcattcgtgt tattgagcca
      361 gtaaaacgta ccactcgcgc ttatcgtgaa gaggcaatta ttaaatccgg tatgaacccg
      421 ttcctgctgg atagcgaaga tgtgtttatc gatttactga ccgacagcgg caccggggcg
      481 gtgacgcaga gtatgcaggc cgcgatgatg cgcggcgacg aagcttacag cggcagccgc
      541 agctactatg cgttagccga gtcagtgaaa aatatctttg gttatcaata caccattccg
      601 actcaccagg gccgtggtgc agagcaaatc tatattccag tactgattaa aaagcgcgag
      661 caggaaaaag gcctggatcg cagcaaaatg gtggcgttct ctaactattt ctttgatacc
      721 acgcagggcc atagccagat taacggctgt accgtgcgta acgtctatat caaagaagcc
      781 ttcgatacgg gcgtgcgtta cgactttaaa ggcaactttg accttgaggg attagaacgc
      841 ggtattgaag aagttggtcc gaataacgtg ccgtatatcg ttgcaaccat caccagtaac
      901 tccgcaggcg gtcagccggt ttcactggca aacttaaaag cgatgtacag catcgcgaag
      961 aaatatgata ttccggtggt aatggactcc gcgcgctttg ctgaaaacgc ctatttcatc
     1021 aagcagcgtg aagcagaata caaagactgg accatcgagc agatcacccg cgaaacctac
     1081 aaatatgccg atatgctggc gatgtccgcc aagaaagatg cgatggtgcc gatgggcggc
     1141 ttgctgtgca tgaaagacga cagtttcttt gatgtgtaca ccgagtgcag aaccctttgc
     1201 gtagttcagg aaggcttccc gacgtatggc ggcctggaag gcggcgcgat ggagcgtctg
     1261 gccgtaggtc tgtatgacgg catgaatctg gactggctgg cttatcgtat tgcgcaggtg
     1321 cagtatctgg tcgatggtct ggaagagatt ggcgttgtct gccagcaggc gggcggtcac
     1381 gcggcattcg ttgatgccgg taaactgctt ccgcatatcc cggcagatca gttcccggca
     1441 caggcgctgg cctgcgagct gtataaagtc gccggtatcc gcgcggtaga aattggctct
     1501 ttcctgttag gccgcgatcc gaaaaccggt aaacaactgc catgcccggc tgaactgctg
     1561 cgtttaacca ttccgcgcgc aacatatact caaacacata tggacttcat tattgaagcc
     1621 ttcaaacatg tgaaagagaa cgcgtcgaat attaaagggt taacctttac ctacgaacca
     1681 aaagtattgc gtcacttcac cgcaaaactg aaagaagttt aattaatact actgagtggc
     1741 tataaggatg ttagccactc tcttacccta catcctcaat aacaaaaata gccttcctct
     1801 aaaggtggca tcatgactga tcaagctgaa aaaaagcact ctgcattttg gggtgttatg
     1861 gttatagcag gtacagtaat cggtggaggt atgtttgctt tacctgtgga tcttgccggt
     1921 gcctggtttt tctggggtgc ctttatcctt atcattgcct ggttttcaat gcttcattcc
     1981 gggttattgt tattagaagc aaatttaaat tatcccgtcg gctccagttt taacaccatc
     2041 accaaagatt taatcggtaa cacctggaac attatcagcg gtattaccgt tgccttcgtt
     2101 ctctatatcc tcacttatgc ctatatctct gctaatggtg cgatcattag tgaaacgata
     2161 tcaatgaatt tgggttatca cgctaatcca cgtattgtcg ggatctgcac agccattttc
     2221 gttgccagcg tattgtggat aagctcgtta gccgccagtc gtattacctc attgttcctc
     2281 gggctgaaga ttatctcctt tgtgatcgtg tttggttctt tcttcttcct ggtcgattac
     2341 tccattctgc gcgatgccac cagctccact gcgggaacgt cttacttccc gtatatcttt
     2401 atggctttgc cggtgtgtct ggcgtcattt ggtttccacg gcaatattcc cagcctgatt
     2461 atttgctatg gcaaacgcaa agataagcta atcaaaagcg tggtcttcgg ttcgctgctg
     2521 gcgctggtga tttatctctt ctggctctat tgcaccatgg ggaatattcc gcgagaaagc
     2581 tttaaggcga ttatttcctc aggcggcaac gttgattcac tggtgaaatc gttcctcggc
     2641 accaaacagc acggcattat cgagttttgc ctgctggtgt tctccaactt agctgtcgcc
     2701 agctcgtttt ttggtgtcac gctgggattg ttcgattatc tggcggacct gtttaagatt
     2761 gataactccc acggcgggcg tttcaaaacc gtgctgttaa ccttcctgcc acccgcgttg
     2821 ttgtatctga tcttcccgaa cggctttatt tacgggatcg gcggtgccgg actgtgcgcc
     2881 actatttggg cggtcattat tcccgcagtg ctggcaatca aagctcgcaa aaagtttccc
     2941 aatcagatgt tcacggtctg gggcggcaat cttattccgg cgattgtcat tctctttggt
     3001 ataaccgtaa ttttgtgctg gttcggcaac gtctttaacg tgttacctaa atttggctaa
     3061 atccttcaag aagccagtca ttcgctggct tcttgcctct caggaaatca cttatgtcca
     3121 aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt actatcagga
     3181 cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta ctttatcccg
     3241 ctgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc aatgccagcg
     3301 aagcgcagtt acatattgcg ttctccgttt atctggcagg tatggcagct gcgatgttat
     3361 ttgccggtaa agtggccgat cgttcaggga gaaaaccggt cgccatcccc ggcgcggcgc
     3421 tatttattat cacttcggtg ttctgttcac tggctgaaac cagcacgtta tttcttgcag
     3481 ggcgctttct acaggggttg ggcgcgggct gttgttacgt ggtggcattc gccattttgc
     3541 gcgacacgct ggatgatcga cgtcgggcaa aagtgctgtc gttactcaac ggcattacct
     3601 gcatcattcc ggtgttagcg ccggtgctcg gtcatctgat tatgcttaaa ttcccgtggc
     3661 agagtctgtt ctggacgatg gcaataatgg gcatcgcggt actgatgttg tctttgttta
     3721 ttttaaaaga aacgcgccct gccgcccccg cagcttcgga caaatcacga gaaaatagcg
     3781 agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc agcgtttcgg
     3841 tgatcctcac cttcgtcaat acatcgccgg tattgctgat ggaaatcatg ggttttgagc
     3901 gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc gtttcatttt
     3961 ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc tcacaggtgt
     4021 tattcctggc ggcgggcatc actcttaccg tttcaccttc ccatgcggtt tctctgtttg
     4081 gtatcacgct gatttgcgcc ggtttctcgg taggttttgg tgtggcgatg agtcaggcgt
     4141 tagggccgtt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt gcgcaggttt
     4201 gcggttcgtc actgtggatt tggctggcag cggtggttgg tatcagcgca tggaatatgc
     4261 tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg ttcgtcgcgc
     4321 ctggacgccc ggttaccgct catgaagaaa tccatcacca cgcttgatct caatctgctg
     4381 ctctgtctgc agctgctgat gcaggagcgc agcgtaacca aagcggcgaa gcggatgaac
     4441 gtgacacctt cggcggtgag taagtcgctg gcaaagttaa gagcgtggtt tgacgacccg
     4501 ctctttgtga actcaccgtt ggggctgtcg cccacaccgc taatggtcag catggagcaa
     4561 aatctggcgg agtggatgca aatgagcaac ctgctgctgg ataaaccgca ccaccagaca
     4621 ccgcgcggcc tgaagtttga gctggcggca gaatcaccgc taatgatgat catgcttaat
     4681 gcgctgtcga aacagatcta ccaacgttac ccgcaggcga ccatcaaatt acgtaactgg
     4741 gattacgatt ccttagatgc cattactcgt ggtgaagtgg atatcggttt ttccggtcgc
     4801 gaaagtcatc cccgttcgcg ggagctgtta agctcgctac cgttagccat tgattatgaa
     4861 gtgttgttta gtgatgtgcc ctgcgtctgg ttacgcaaag atcatccggc actgcatgaa
     4921 aagtgggatc tcgatacctt tttacgttat ccgcatatca gcatttgctg ggaacagagc
     4981 gatacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgtac gattgcaatg
     5041 agcctgccgg aattcgagca gtcactgttt atggcagcgc agcccgacaa tctgctcctg
     5101 gcgaccgcgc cgcgctactg tcagtactac aatcaactcc accaactgcc gttggttgct
     5161 cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagtcccttt taccctgctg
     5221 tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggatac cattaaaaat
     5281 ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccgatttc acaataaaat
     5341 gtaaaaaagt tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc
     5401 attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg
     5461 gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc
     5521 tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg
     5581 tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa
     5641 atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct
     5701 atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc
     5761 gatatggaac tgcagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac
     5821 acattttcca gcaatgaatc gctatgggtc agtaaacaaa atgatccaaa cgaagcgcgg
     5881 gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac
     5941 gatccgcgcg atctgcaact gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat
     6001 catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc
     6061 acgattgaaa agctcagtgt ctgggagtta gatggcaaac acggctggaa aagcctgccg
     6121 gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct
     6181 accatgaagg ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa
     6241 ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca
     6301 cgtaccctac cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc
     6361 gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa
     6421 gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac
     6481 tattcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa
     6541 ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg
     6601 cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag
     6661 ccggaattta tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt
     6721 gatcagggta cgctggacca cctgaccggg caattgaccg catttggcga gtttattcag
     6781 cgagttaaga tctaaataaa aaacccgcca gcaatcatgc atggcgggtt tttaacgcgc
     6841 tatcgatttt agtgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg
     6901 attacgcacg ggctaaggtc acgaatacgc ccggtaccaa tcttcatcac gcagtaggag
     6961 ataaagccca gcgcaatacc ttcggtaatc gagaagctga acggcatcat cacagcggta
     7021 ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc
     7081 atcaacacgc caacgtaaat cagcgcgcca gctgcagcgt agcctggcac cattcccgcc
     7141 agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg
     7201 gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta
     7261 ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc
     7321 gggaatttcc ccttctcatc tgccagaccg gctttatcgg tcacgccaat caacgtgccg
     7381 gaggagtcaa acaggttgac cagcatgaaa gagaaaatca cccctgccag cccgaggtta
     7441 aacgacccgg ctaaatctac atgacccacg acagtcatta cgctcggcgg cgcagaaacg
     7501 atgccattgt agtggacatc acccagcatc cagcccagca gcgttgtcac cacgatagaa
     7561 accagcaccg ctgcgtgaat gttgcgtgag gccagaatag caatgatgaa gaagccgagg
     7621 atccccagca gcacgctgtg agaagtcaga ttaccgatgc tgaccagcgt ttccgggtta
     7681 gcgacaatca cacctgcgtt tttcagcccc atcatgccaa tgaacagacc gataccgctg
     7741 gtaatgccca cacgcagact caccggaatg ttggcaatca tccagtagcg aacgcggaaa
     7801 atcgtcagta acagcagacc aatcgcgccc cagaagattg cgcccatccc gacttgccac
     7861 ggcaagccca tcgcctgcac aacgacaaaa gcgaagaacg cattcaggcc catagcgggt
     7921 gccagtgcaa ctggcaggtt agcaaacagc cccatcataa tactgccgaa tgcagcaatc
     7981 agacaggtag tgacgaagac ggcgctggta tccatgccag caacgccaag aatttgcggg
     8041 ttaacaaaaa cgatgtaaac catcgtcagg aaggtggtaa aaccggcgat cacttcggtc
     8101 cgtgccgtcg tgccatgttc gcgcagttta aacacgcgtt ccagcatccc ctggccagaa
     8161 gcctgggttg tgtgttgttg actcattatc tatttccgaa caaggaggga aaatccgtcg
     8221 ctatcgtata ccaaaatgag acaatatgga cggctgaaga taactttttt cattgtattt
     8281 gcttatacgg caacgattgc gtcatgcgaa ttggcatgac gaaaacgtta aactaacgat
     8341 aaaggaaaga catgtcccag atagaagcgg tatttttcga ctgcgacggt acgctggtcg
     8401 atagcgaggt catttgctct cgcgcctatg tcacgatgtt cagggaattt ggcattcacg
     8461 ttgacctgaa agagattttt acccgtttta aaggcgtaaa actctacgaa atcatcgata
     8521 tcatcagtaa agaacagggc gtgacgatgg tccaggccga tgccgagcac gtttaccgcg
     8581 cagaagtagc acgtctgttt gataccgaac ttgaggacat cgcaggagcg aacgaattgc
     8641 tggcgagcat taacgtgccg atgtgcgtcg tctctaacgg tccggtcagc aaaatgcagc
     8701 attcgcttgg taagctcaac atgctgcact acttcccgga aaaactgttc agcggatatg
     8761 acattcagcg ttggaagccc gatccggcat taatgttcca tgcggcaaaa gcgatgaatg
     8821 tgaacgttga aaactgcatt ctggtcgatg actccagcgc tggcgctcaa tcagggatag
     8881 atgccggaat ggaagtgttt tacttctgcg ctgacccgca caataagccg atcgttcacc
     8941 cgaaagtcac cacctttacc gagctgtcgc agctgcctgc gctctggaag gcacgcggct
     9001 ggaatataac gcgataatcg cctccttact tcaacactta cacatcagtc acgctggttt
     9061 gagatacaga acaccaggtg tctgatgagt aagcgtctat tccctatgaa taatttatgt
     9121 cgttattcgt cctgtcatcc accctctgta tggttaagcc tgggttatat tttgtacaat
     9181 atcgggatac cgttcgaacc aatccagaat attacgcatc gcgtcactga tgtttttatc
     9241 gtctttatat tgattctcaa gtctattatt tcgaacaata gtaagcttag aacgtatttt
     9301 atcatcacaa tcgacaattt tcgcattcaa tgcattta
//
LOCUS       DAZFDC010000153         9332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFDC010000153 DAZFDC010000000
VERSION     DAZFDC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.153"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1386
                     /locus_tag="ACHJ48_004124"
     CDS             <1..1386
                     /locus_tag="ACHJ48_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFO4709571.1"
                     /translation="RIAGISRDDGLITLQDAEGNTRLISPREAVAEGVTLYPPDTIRV
                     GTGDRMRFTKSDRGRGYVANSVWTVTAVSGDSVTLSGGQQTRVIRPAQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGRTEQA
                     VREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVR
                     DLQKEKTLGGD"
     gene            1406..2152
                     /gene="traX"
                     /locus_tag="ACHJ48_004125"
     CDS             1406..2152
                     /gene="traX"
                     /locus_tag="ACHJ48_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO4709572.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            2211..3071
                     /locus_tag="ACHJ48_004126"
     CDS             2211..3071
                     /locus_tag="ACHJ48_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4709573.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            3174..3734
                     /locus_tag="ACHJ48_004127"
     CDS             3174..3734
                     /locus_tag="ACHJ48_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO4709574.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTQDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3867..4079
                     /locus_tag="ACHJ48_004128"
     CDS             3867..4079
                     /locus_tag="ACHJ48_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO4709575.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            4324..4785
                     /locus_tag="ACHJ48_004129"
     CDS             4324..4785
                     /locus_tag="ACHJ48_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO4709576.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRMLGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            4831..5040
                     /gene="hha"
                     /locus_tag="ACHJ48_004130"
     CDS             4831..5040
                     /gene="hha"
                     /locus_tag="ACHJ48_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO4709577.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            5078..5416
                     /locus_tag="ACHJ48_004131"
     CDS             5078..5416
                     /locus_tag="ACHJ48_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709578.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTG
                     AAQQSPEHRS"
     gene            5656..5910
                     /locus_tag="ACHJ48_004132"
     CDS             5656..5910
                     /locus_tag="ACHJ48_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO4709579.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCV"
     gene            6146..6220
                     /gene="tap"
                     /locus_tag="ACHJ48_004133"
     CDS             6146..6220
                     /gene="tap"
                     /locus_tag="ACHJ48_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO4709580.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            6213..7070
                     /gene="repA"
                     /locus_tag="ACHJ48_004134"
     CDS             6213..7070
                     /gene="repA"
                     /locus_tag="ACHJ48_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO4709581.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRLRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            7982..8266
                     /locus_tag="ACHJ48_004135"
     CDS             7982..8266
                     /locus_tag="ACHJ48_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO4709582.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            8266..8541
                     /locus_tag="ACHJ48_004136"
     CDS             8266..8541
                     /locus_tag="ACHJ48_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4709583.1"
                     /translation="MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            8636..8842
                     /locus_tag="ACHJ48_004137"
     CDS             8636..8842
                     /locus_tag="ACHJ48_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709584.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLDR"
     gene            complement(8942..9157)
                     /locus_tag="ACHJ48_004138"
                     /pseudo
     CDS             complement(8942..9157)
                     /locus_tag="ACHJ48_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9201..>9332)
                     /locus_tag="ACHJ48_004139"
     CDS             complement(9201..>9332)
                     /locus_tag="ACHJ48_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4709585.1"
                     /translation="NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT         2421 a         2190 c         2594 g         2127 t
ORIGIN      
        1 cggattgccg gtatcagcag ggatgacggg ctgataactc tgcaggatgc ggaaggtaac
       61 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacactgta ccccccggac
      121 accatcaggg tggggaccgg tgaccggatg cgcttcacga agagtgaccg ggggcgtggt
      181 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacgctgtcg
      241 ggcggacagc agacccgggt gattcgcccc gcccaggagc gggcagagca acatattgac
      301 ctggcctatg ccatcaccgc tcacggtgcg cagggggcaa gtgaaacctt tgccatcgcg
      361 cttgaaggta cggaaggcag ccggaaactg atggccggct ttgagtcagc ctacgtggcc
      421 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
      481 gccattaaca atgccgtaca gaaaggaaca gcccacgatg tgtttgaacc gaaaccggac
      541 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
      601 gcagggcgtg ccgttcttcg tcaggcgggg cttgccgggg gagacagtcc tgcacggttt
      661 attgctccgg gacgtaaata tccacagccg tatgtggcac tgccggcgtt tgaccgtaac
      721 ggcaagtctg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
      781 ggattcagtg gtgaaggacg ggtgaaaggc agcggggatg cgcagttcgt ggccctgcag
      841 ggcagccgta acggagagag cctgctggct gataatatgc aggatggtgt ccggattgcc
      901 cgtgataatc ctgacagcgg tgtggtggtg agaatcgccg gtgaaggtcg tccgtggaat
      961 cccggtgcca tcaccggtgg tcgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     1021 ggagccggaa atggcgagcc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     1081 gccatccgcc gtgaaacgga acgccgcgca gatgaaattg tccgtaaaat ggcagagaac
     1141 aaacctgacc tgccggacgg cagaacagag caggctgtca gggagattgc cgggcaggag
     1201 cgtgaacggg ctgtcacttc tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     1261 ccacaacggg agcgggaggc ggtccgtgag gttgtccggg aaaacctgct gcaggagcga
     1321 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cctgggcgga
     1381 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     1441 ttcactggct gcccggaccg atacctggtt gcagtcgttg ctggtctggt cacccggaca
     1501 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccacg caaaccgcat
     1561 tctgcatctg gaccagacgt ggatgtttct ggccgggcgt ggagcctttc cgttgtttgc
     1621 tctggtgtgg gggctgaatc tgtcccgcca tgcgcatatc aggcagtcgg ccattaaccg
     1681 gctgtgggga tggggaatta ttgcccagtt cgcgtattat ctggcaggct ttccgtggta
     1741 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     1801 gcgcagtggg tggcgtacag cagcggccat tctcctgatg gcactgtggg ggcctttgtc
     1861 cggcaccagt tacggcattg ccgggctgct gatgctggca gtcagccacc ggctgtaccg
     1921 ggcggaagac agaacggaac gtctggtgct ggttgcatgc ctgctggccg tgattccggc
     1981 acttaacctt gcctccagtg atgcggcggc tgtagctggt cttgtgatga cggtgctgac
     2041 cgttggtctg gtgtcctgtg cagggaaatc attaccccgt ttctggcccg gagatttttt
     2101 cccgacgttc tatggctgtc atctggctgt gctgggcatt ctggcgttgt gactggtatg
     2161 atactgacgc tgattctgct tattaataaa caacataaca caggatattt atgaaaatta
     2221 cggatcacaa actctctgaa ggtattgccc tgacatttcg tgttcctgaa ggaaatataa
     2281 aacatccttt gattatctta tgccatgggt tttgcggtat caggaatgtt ctgctccctt
     2341 gctttgcgaa tgccttcaca gaagcaggat tcgcaacgat tacttttgat tatcgaggat
     2401 tcggtgagag tgatggagaa cgagggcgtc tggttcctgc aatgcagacc gaagatatta
     2461 tttcagttat caattgggca gaaaagcagg aatgtattga taatcagcgt attgggttat
     2521 ggggaacatc gctgggaggg gggcatgtct tcagtgcagc tgcgcaggac caacgcgtaa
     2581 aatgtattgt cagccagctg gcttttgctg acggtgatgt tctggttacc ggagagatga
     2641 atgaatcgga gagagcgtct tttctatcca cgctgaataa aatggcagag aagaagaaaa
     2701 acaccgggaa agagatgttt gtgggggtta cacgggtact aagtgatgat gaatcgaagg
     2761 tattcttcga gaaaatcaaa gcccggcatc ccgaaatgga tataaaaata cctttcctta
     2821 cggtcatgga aacgttgcaa tataaaccgg cagagagtgc agccagcgtc cagtgcccag
     2881 ttttggttgt tattgccggc caggatagcg ttaatccacc ggaacaggga agagcattat
     2941 atgatgctgt tgcttctggt actaaggaat tatacgagga agctgatgca tgccattatg
     3001 atatttatga gggcgcgttt tttgaacgtg tagtagctgt gcagacacaa tggtttaaac
     3061 aatatcttta attgtcagta tttatacgtt aaaggtcgtt gaaattaact tcctgttacg
     3121 atgagccgtt tcaacggcct gcagggcata ttttcacgaa ggtcagagta agcatggcag
     3181 agcaaaaacg accggtactg acactgaagc ggaaaacgga aggagagacg cctgtccgca
     3241 gccggaaaac catcatcaat gtcaccacgc caccaaaatg gaaggtgaaa aagcagaagc
     3301 tggccgagaa agcagcccgg gaagcggagc tggcggcaaa aaaagcgcag gccagacagg
     3361 cgctgtccat ttatctgaac ctgcccacgc aggatgaggc cgtgaacacc ctgaaaccct
     3421 ggtggccggg attatttgac ggtgacacgc cccggcttct ggcctgcggt atccgggacg
     3481 tgttactgga agacgtggcg cagcggaata tcccgctctc gcataaaaag ctgcgcaggg
     3541 cgctgaaggc catcacccgt tcagaaagct atctgtgtgc catgaaagcc ggtgcctgcc
     3601 ggtatgacac ggaagggtat gtgacggagc atatttctca ggaggaggaa gcgtatgcgg
     3661 cagcgcgtct ggataaaatc cgccgccaga accggataaa ggcagaactt caggccgtgc
     3721 ttgatgagaa ataaaaaaag cctccctcag agagaggagg cagggaaata aggcttaaaa
     3781 ggaaataact tccgtacccg aaaggaagcc aattcagtag attatttaac accataatac
     3841 tgacgggtta aagaggagtg ataaagatga atggattcag aaacagttca cggaacggtc
     3901 aggtctggcg ttaccagcgt gccggaagcc gggccgttat tctggaagtc agtggacgct
     3961 ggatggaagc ggcagaagca tggcgacggg ctgccggtgt tgcaccccgg actgactggc
     4021 agcagtttgc ccgaaaaagg gctgaacact gccaccggcg ctgccgagga agggggtaac
     4081 gaaaaaagca gccttcatta ctccagggat tcagaaggct gaataatgca tgagatttgc
     4141 tttttttaat atccggtcgg gcagtatgac cggccagttc tgagtagcac agagtttttt
     4201 ccgtattaag tcgtgatttt atattttgtg attaatttca caaaataagg tgttattaag
     4261 tgtgtgctgc aatattcagg atgcctgaaa tacaggtgct atttatctga tacggagaat
     4321 aacatgagga aatatattcc actggtatta tttatctttt catggccggt attgtgtgca
     4381 gatattcatg gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca
     4441 cggaaggcgg tgcggatcag gctggtaaat attgatgcgc cggaaaagaa acaggattat
     4501 ggccggtggt cgacagacat gatgaaatca ctggttgcag gaaaaacggt taccgtcaca
     4561 tattttcagc gagatcgtta tggtcgtatg ctcgggcagg tttatgcacc agacggaatg
     4621 aatgttaatc agtttatggt ccgggccggg gccgcctggg tatatgagca gtacaacaca
     4681 gatcccgtgc tgcctgtcct tcagaacgaa gccagacaac aaaaacgtgg gctctggtca
     4741 gatgctgatc cggttccccc ctggatatgg agacaccgta aataaagcga tggcaatctt
     4801 atcattcatc tgttctcagc gggaataaaa atggaaaaaa caaaacagga atggctgtat
     4861 caattacggc gatgttcatc agtcaatacg ctggaaaaaa ttattcataa aaacagagac
     4921 agtttatcga ccagtgaacg tgaatcattt aattcagcag cagaccaccg gttagcagaa
     4981 ttaatcaccg gaaagttata tgacaggata ccaaaagaaa tatggaaata tgtaaggtaa
     5041 tagttcagca taattaatta ccaggagcag ataaacaatg aagctgatta tttttattct
     5101 cattgttctg attattgcag cgttacttat ccggattatc ctcagaagtg taaatcagca
     5161 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg accggtgatg cagagcgaat
     5221 actgtccggc ggtgaatatg atgccagcca tttacgacct gaacgcagac gccgggatat
     5281 tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc acgatgccag
     5341 aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgctc agcagtcacc
     5401 ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa
     5461 gcaagttttc cccgctcccg gcgtttccat aactgaaaac catactattt cacagtttaa
     5521 atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc ttcgtcacaa
     5581 ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc cctgtttaag
     5641 tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatc tggcgcaaaa
     5701 cgagcataca gaaaggggaa tccgctttct gatgcagaga aacaaagatt atcagtggcc
     5761 cgtaaaagag cttcgttcaa ggaagtaaaa gtatttcttg aaccaaagta taaggccatg
     5821 ctcatgcaaa tgtgtcatga agatggcctg actcaggctg aagttctgac cgcactgata
     5881 aaaagtgaag cgcaaaaacg atgtgtgtga tgatgggctt acattcttga gtgttcagaa
     5941 gattagtgct agattactga tcgtttaagg aattttgtgg ctggccacgc cgtaaggtgg
     6001 cagggaactg gttctgatgt ggatttacag gagccagaaa agcaaaaacc ccgataatct
     6061 tcagaaagtt tggcgactga gaagattacc ggggcccact taaaccgtat agccaacaat
     6121 tcagctatgc ggggagtata gttatatgct cggaaaagtt caagacttct ttctgtgctc
     6181 gctccttctg cgcattgtaa gtgcaggatg gtgtgactga tcttcaacaa acgtattacc
     6241 gccaggtaaa gaacccgaat ccggtgttca caccccgtga aggtgccgga acgctgaagt
     6301 tctgcgaaaa actgatggaa aaggcggtgg gcttcacctc ccgttttgat ttcgccattc
     6361 atgtggcaca tgcccgttcg aagggactgc gtcggcgcat gccaccggta ctgcgtcgcc
     6421 gggctattga tgcgctgctg caggggctgt gttttcacta tgacccgctg gccaaccgtg
     6481 tccagtgctc catcaccaca ctggccattg agtgcggact ggcgacggag tctgctgccg
     6541 gaaaactctc catcacccgt gccacccggg ccctgacgtt cctgtcagag ctgggactga
     6601 ttacctacca gacggaatat gacccgctta tcgggtgcta cattccgacc gacatcacgt
     6661 tcacaccggc tctgtttgct gcccttgatg tgtctgagga tgcagtggca gctgcgcgcc
     6721 gcagccgtgt tgaatgggaa aacaggctgc gcaaaaagca ggggctggat accctgggta
     6781 tggatgaact gatagcgaaa gcctggcgtt ttgtgcgtga gcgtttccgc agttaccaga
     6841 cagagcttaa gtcacgggga ataaagcgtg cccgtgcgcg tcgtgatgcg ggcagggaac
     6901 gtcaggatat cgtcaccctg gtgaaacggc agctgacgcg tgaaatctcg gaagggcgct
     6961 tcactgctaa tcgtgaggcg gtaaaacgcg aagtggagcg tcgtgtgaag gagcgcatga
     7021 ttctgtcacg taaccgcaat tacagccggc tggccacagc ttccccctga aagtgacctc
     7081 ctcagaataa tccggcacgc gccggaggca ttcgcacgcc tgaagcccgc cggtgcacaa
     7141 aaaatcagca ccacatccca aaaacaacct catcatctac cttctggtgc atccggtccc
     7201 ccctgttttg atacaaaaca cgtctcacag acgggcaatt ttgtttatcc acattaaact
     7261 gcaagggatt tctctgtaag attacaaccg ttcatgtcat aaagcaccag ccgccaggct
     7321 tacagggtgc aatgtatctt ttaaacaccc gtttatatct cctttaaact acttaattac
     7381 attcatttaa aaaaaagact tattcactgc ctgtcctatg gacagacaga tatgcacctc
     7441 ccaccgcaag cggcgggccc caaccggagc cactttagtt acaacacaca aaaacaacct
     7501 ccagaaaaac cccggtcccg cgcagaactg aaaccacaaa gcccctccct cataactgaa
     7561 aaacggcccc gccccggccc aaagggccgg aacagagtcg cttttaatta tgaatgttgt
     7621 aactaagcat catcatcgct gtcagtcttc tggctgaacg ttctcagtac acgctcgtaa
     7681 gcggccctga gggctcgcta acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg
     7741 gaccactccg accgcgcaca taaactctct catggctgaa agcgggtatg gtctggcagg
     7801 gctggggggg ggtaaggtga aatccatcaa tcagtaccgg cttacgccgg gcttcggcgg
     7861 ttttacacct gtgctacatg taacctccgt ataccgcctt acatgcgctg acgcgcatta
     7921 tttgccttgt tccgtctgaa agtaatcact atgattaaat atgattaacg actaatcgga
     7981 tatgcaaatg aaaaacaata ccgcacaagc aacaaaagtc attaccgcgc atgtgccatt
     8041 acctatggct gataaagtcg accagatggc cgccagactg gaacgctccc ggggttgggt
     8101 tatcaaacag gcgctttctg catggcttgc ccaggaggag gagcgtaatc gcctgacgct
     8161 ggaagccctg gacgatgtga catccggaca ggttattgac catcaggctg tacaggcctg
     8221 ggcggacagc ctcagtactg acaatccgtt accggtgcca cgctgatgga actgaagtgg
     8281 agcagtaaag cgctttctga tttggcgcgg ttatatgatt ttctggtgct agccagtaaa
     8341 cctgctgccg ccagaacggt acagtccctg acacaggcac cggtcattct gttaactcat
     8401 ccacgtatgg gggaacagtt atttcagttt gaacccaggg aggtcagacg gatttttgct
     8461 ggcgagtacg aaatccgtta cgaacttaat ggccagacta tttatgtatt gcgcctgtgg
     8521 cacacacgag aaaacaggta gattgtgtgt ggggacaact taacatagcc tgcagatatt
     8581 aattctggaa cattattatc aggccagata tttaaataat cttgtaggag ataaaatgaa
     8641 ttacgatgaa ataacgaaaa ttaccgctga acgtattagc gactacatga ctgaagcagt
     8701 taatacagac tctatagccg ttgcggaaat gtttcataac gcagcatggg gagtgcgaac
     8761 attgtggttt gaactcgtca caaaaataga tatagatata cataaaaaga acagatatgc
     8821 gagttatgat cttgatcgat aggaatttaa atcccaaaaa gattaaaaaa acacccaaaa
     8881 acggatgttt attcaacact gcctgcgtgc catgagtgca ccgccgtcaa ttaaatggat
     8941 ctatttcatc ctgaccagca tagtgctcca gacggctcat cggcaccggc ggttttttca
     9001 ggtaagagcc gaagtacacc gccacatgtg tggcgttatc catcacccgg gatacgttga
     9061 cattccagcc ccgccggtaa tgactgtcca ggaagcgatt ccagtcccgt ttactgcttc
     9121 cttctgctgc cagcgcatcc ggtatcacca ggtccggtga ggtgtactgg caatagcgga
     9181 cactaccatt tgttcttttt ttaagcagcc atctgatgat atttttccct gaaggctgcc
     9241 ggggagatat tccccagacg agagtgacga cgctgacgat tgtagaaaat ctcaatgtat
     9301 tcccgtatta ctgagatggc ttcatcccgg tt
//
LOCUS       DAZFDC010000154         9193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFDC010000154 DAZFDC010000000
VERSION     DAZFDC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.154"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(114..437)
                     /locus_tag="ACHJ48_004140"
     CDS             complement(114..437)
                     /locus_tag="ACHJ48_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO4709586.1"
                     /translation="MKRTLLIAVWAIGLMSDSAMALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(445..630)
                     /locus_tag="ACHJ48_004141"
     CDS             complement(445..630)
                     /locus_tag="ACHJ48_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO4709587.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(627..3266)
                     /locus_tag="ACHJ48_004142"
     CDS             complement(627..3266)
                     /locus_tag="ACHJ48_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO4709588.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPSVKLVGEFTMPLMPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
     gene            3474..4463
                     /gene="ldhA"
                     /locus_tag="ACHJ48_004143"
     CDS             3474..4463
                     /gene="ldhA"
                     /locus_tag="ACHJ48_004143"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO4709589.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4574..4996
                     /gene="hslJ"
                     /locus_tag="ACHJ48_004144"
     CDS             4574..4996
                     /gene="hslJ"
                     /locus_tag="ACHJ48_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO4709590.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTCDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4993..5259)
                     /locus_tag="ACHJ48_004145"
     CDS             complement(4993..5259)
                     /locus_tag="ACHJ48_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO4709591.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5533..9057
                     /gene="nifJ"
                     /locus_tag="ACHJ48_004146"
     CDS             5533..9057
                     /gene="nifJ"
                     /locus_tag="ACHJ48_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO4709592.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPAFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2289 a         2407 c         2260 g         2237 t
ORIGIN      
        1 aggatatata aggtttttgt agaaataccc acggtaatta agcatgctaa cgtcagttta
       61 aatactgtaa aagatcatta cattaataga tacattttgt agccaactat aatttatttg
      121 cgcaaccatt taccattaac tccctgaaca tattgccccg acttcgcgcg agcaacaagc
      181 ttttgtccag ccagtttggc tatctcttta gtggatacat tattttgctt tgccagttgt
      241 tggtaactat ggttacgagc ttcattaata tcttttacta atgcctgtgt ttcggcatcc
      301 gtttgtagcg ccaccaggta accgttaagc gtttcaccta cccgcccctg acttctggct
      361 tcgttcaggg ttaaagccat tgcactgtcg ctcatcaggc caatggccca tacagctatg
      421 agtaatgttc ttttcatcat cacctcaaaa tagatcgcta cgggttttga gcaggtcttc
      481 aacgtcctta tccgctttaa tgatgatttc atgctcaatt ttaacgttca tattaatggt
      541 gattggctct ttcggcgcag ccacttcaat gcgaggcgtg caaccaacca acataaaaga
      601 ggacgcaaac gcggctatca ctattttcat tgtttttcct cacactcttt tcctgatggg
      661 cagccagttt ccggtagctg ggcattctgc tccagccata cctggagatt atcgccaaag
      721 cgtaaactgc gccataacgt aaaaatattc tcttcatggt gataattaag gtttaccgta
      781 ccgcttttac catcgacgcg actggtgccc gaaatgttgg cctgcatggt taacacaccg
      841 agattatcta aattaatcct tgttgatgag cgactgattt ccatataacg caaccagttg
      901 atggcagttc cagcggtcat attgtctttg gccaccgcat ctgcagtgtc tttatccaga
      961 cgcagcgtca tcggccccgc gtttgccaac cagccatctt tgacaatcca tttttcattg
     1021 ttcagccata gtggcaacgc cccactaaat gccccagaca tagcaaactg tttcggatta
     1081 accgcgctaa ccagttcgct ggatgagaga ttattcagcc gcaacagcgc cgggtcatgt
     1141 tgcggcatac gtaattgttt catcagcacg ttaccgccta acacatcgac gctaacatca
     1201 gtcaacagca atggttcttc ttcagtccac ggataacgcc cttgcaaatc agccgtaata
     1261 tttttcgctg tcaccagatt aatcacttcg gcaattcgca acgtaacggg gccgcgagtc
     1321 cccagatgcc aggctccatc ggcaaaacgg aaaggcagga caaaatcgac accgttaacc
     1381 tgattatctg gcatccaggc actaccgcct ttcaacacgc cgtgccctcc cgcgcggaat
     1441 ccttgttcag gcgcagcaga aaatgcaacc tgagcataca gttcaccatc gcgtaagttc
     1501 atcttccagt cgggtggcac cagcggctgg aatacagtca gtgattgttt aggccaccag
     1561 gcgttaccgc gcagacgaat accgtcccag cgaccattta cccgaaccgg gccaatctca
     1621 ccagcatgta aatcgccttt aaagaggaaa tacgtcggat cgcgcccatc gacgctaaat
     1681 tttaaggttg atggcggtaa cacactgccg ccagtgaaca gcgtttgccc ggcgtccagt
     1741 gacagcgcgc cgctgaagga gggatgctgt gcgtcacgta cccagacgac gggcttgtcg
     1801 agaattaatc gcggcttttc taccgtcatc gtaccgtatt gtaactggtc gaaaccggtg
     1861 gagagatcgg tcagcgtaat cgtgctgtca tgccactcac cttttcctgc gacatcccag
     1921 gtggcattca tcggtgtaaa actccctttt ccccagtagc gccactgcca gcggccagcg
     1981 tcagggagaa aatcattcgc cagcccatcc atatgcagca cgaaatcgcc cagttcattt
     2041 tcatgcgcct gcaaaatggc ctgcaaacgt ccgtcaacac cacgttgggt gacttttaca
     2101 cccgctaaag gccagcggat ttcatcgatg tccagcgaat cgatgactct tccctttgaa
     2161 cgaagtaacg cgcctggctc aaaggtcagc gttgggtcag acagacttcc acttagctgc
     2221 gcaggtaaac gggcatataa aatgaggtcc gcctgtttcg cttcaccggt cagctgcaga
     2281 ggcagctggc tgttatccat gcttaatttt cctgggccaa aattaagcac cgcgttgccc
     2341 ttacccgctt gcccctgggt cagcacactc agtcgtccgc tgaccagagc gttctcaagc
     2401 cctgcctgcc agttgtcgac tttgacaccc agtcggccac tcaaaggaaa acctgcatac
     2461 ggccagctcc agcgaccatc gcttacggtc aattgttgac gagtaatttg ccacggcaaa
     2521 tcgagcaacg gatcgccgtt atcccgtgcc agcacgatca attgcccgct attttcctgc
     2581 cagtccagct cggcatccac cagtgacggt tcctgcggca agtttaacgt cgcagtagca
     2641 tgcccactta caggaagtcc atccggcatg agcggcatag taaattcccc caccagttta
     2701 accgacggct gattttcaaa cgcgacgaca tccagttcgc tgactgtaag ttgttgccct
     2761 ttcagctggc cttgaaattt aactttttcg ccctgataac gcagttgctg gatatcagag
     2821 gttaatgcga gagagagttt tccctgccat tcctgccagg gagaaaaaat cagtttatca
     2881 atattgatcc aggtgttagg cagcatggac tgccattgcg cgagggtttt tggagcagct
     2941 ggcgattgct ccgtctgcgg caatttcgcc agacaagcag aatcaagttc taccgtgccg
     3001 acgttcaata accagcggct gggatgtgaa aggctggcgt tggtgatatg cgcaagctga
     3061 caatctccca ccagataacg gagatcggga atgattaaac ctttacgcgt aatgcgtggg
     3121 ctttcatcta atgcaatacg tgtcccgagc ggtagccaga tgcccgccag cgtgggaacc
     3181 cacagcccga gcgtcatcag cagcgtcaac ggcacaagaa taatcagtaa taacagcgcg
     3241 agaacggctt tatatttacc cagcatgggt agttaatatc ctgatttagc gaaaaattaa
     3301 gcattcaata cgggtattgt ggcatgttta accgttcagt tgaaggttgc gcctacacta
     3361 agcatagttg ttgatgaatt tttcaatatc gccatagctt tcaattaaat ttgaaatttt
     3421 gtaaaatatt tttagtaact taaatgtgat tcaacatcac tggagaaagt cttatgaaac
     3481 tcgccgttta tagcacaaaa cagtacgaca agaagtacct gcaacaggtg aacgagtcct
     3541 ttggctttga gctggaattt tttgactttc tgctgacgga aaaaaccgct aaaactgcca
     3601 atggctgcga agcggtatgt attttcgtaa acgatgacgg cagccgcccg gtgctggaag
     3661 agctgaaaaa gcacggcgtt aaatatatcg ccctgcgctg tgccggtttc aataacgtcg
     3721 accttgacgc ggcaaaagaa ctggggctga aagtagtccg tgttccagcc tatgatccag
     3781 aggccgttgc tgaacacgcc atcggtatga tgatgacgct gaaccgccgt attcaccgcg
     3841 cgtatcagcg tacccgtgac gctaacttct ctctggaagg tctgaccggc tttactatgt
     3901 atggcaaaac ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg ctgcgcattc
     3961 tgaaaggttt tggtatgcgt ctgctggcgt tcgatccgta tccaagtgca gcggcgctgg
     4021 aactcggtgt ggagtatgtc gatctgccaa ccctgttctc tgaatcagac gttatctctc
     4081 tgcactgccc gctgacaccg gaaaactacc atctgttgaa cgaagccgcc ttcgatcaga
     4141 tgaaaaatgg cgtgatgatc gtcaatacca gtcgcggtgc attgattgat tctcaggcag
     4201 caattgaagc gctgaaaaat cagaaaattg gttcgttggg tatggacgtg tatgagaacg
     4261 aacgcgatct gttctttgaa gataaatcca acgacgtgat ccaggatgac gtattccgtc
     4321 gcctgtctgc ctgccacaac gtgctgttta ccgggcacca ggcattcctg acagcagaag
     4381 ctctgaccag tatttctcag actacgctgc aaaacttaag caatctggaa aaaggtgaaa
     4441 cctgcccgaa cgaactggtt taatcttgcc gctcccctgc aatccagggg agctgattca
     4501 gataatcccc aatgaccttt cattctctat tcttaaaata gtcctgagtc agaaactgta
     4561 attgagaacc acaatgaaga aagtagccgc gtttgttgcg ctaagcctgc tgatggcggg
     4621 atgtgtaagt aatgacaaaa ttgctgttac gccagaacag ctacagcatc atcgctttgt
     4681 gctggaaagc gtaaacggta agcccgtgac ctgcgataaa aatccgccag aaatcagctt
     4741 tggtgaaaaa atgatgattt ccggcagcat gtgtaaccgc tttagcggtg aaggcaaact
     4801 gtctaatggt gaactgacag ccaaagggct ggcaatgacc cgtatgatgt gcgctaaccc
     4861 gcagcttaat gaactcgata acaccattag cgaaatgctg aaagaaggtg cacaagtgga
     4921 tctgaccgcg aaccagttaa cgctggcgac cgcgaaacag acattaactt ataagctggc
     4981 ggatttaatg aattaatagc tgccacagct cccggcggca agtgactgtt cgctacagcg
     5041 tttgccgttg ggtaatgcac acatcccaat cgccgtacca tccagttgac gggcaacaga
     5101 aagcgaaccg ccgatcattg cacaatttgc ttctccacta ctggacatcg acgcttttaa
     5161 acctggcgct acgtgcgccg cagtcgcctg ctgaacaggc tcactactac acgccgacaa
     5221 caataaagcg gcacacccta cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca
     5281 agccaaacga caccgccata aataataggc agcacagagg gcgacgtcga gagctgtcct
     5341 gcgcgttgcc ccgcattttt acttttttat ggctattttt ttgccctctg tttgatcaaa
     5401 acattcatta cgctgatgtg ggggacacaa aagcgaaaat gcagaagaaa gccatttgct
     5461 aaaattgaaa gattactact gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg
     5521 taagggtgtc atatgattac tattgacggt aatggcgcgg ttgcttcggt ggcgtttcgc
     5581 accagtgaag ttatcgccat ctaccctatt acccccagtt ccacgatggc agaacaggct
     5641 gatgcctggg ccggaaacgg cttgaagaac gtttggggag acacaccacg cgtggttgaa
     5701 atgcagtcgg aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac gggtgccctt
     5761 tcaacatcgt ttacgtcatc gcagggtttg ctgctgatga tcccgacgct gtacaaactg
     5821 gcaggcgaac tcacaccgtt tgtcctgcat gtagcggcac gtaccgttgc cacacatgca
     5881 ctctctattt ttggcgatca ttccgacgtc atggcggtgc gccagacggg ttgcgcgatg
     5941 ttgtgtgcgg caaacgtcca ggaagcgcaa gactttgctc tcatttcgca catcgcgacg
     6001 ctgaaaagcc gcgtgccatt tattcatttc tttgatggtt tccgtacgtc ccacgaaatc
     6061 aataaaattg tcccgctggc cgatgacact attcttgatc tcatgccgca ggccgaaatt
     6121 gatgctcatc gcgcccgggc gctcaacccg gaacatcccg tgatccgcgg tacgtcagcc
     6181 aatcctgaca cttatttcca gtctcgcgaa gccaccaacc catggtacaa cgcggtctat
     6241 gaccatgttg aacaggcgat gaatgatttc tctgccgcga caggtcgtca gtatcagccg
     6301 tttgaatatt acgggcatcc gcaagcggaa cgggtgatta tcctgatggg ctctgccatt
     6361 ggcacctgtg aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg
     6421 aaagttcgcc tgtaccgtcc cttctccgcc aaacacttgc tgcaagctct gccgggatcc
     6481 gtacgcagcg tggcggtact ggacagaacc aaagaacccg gtgcccaggc agaaccgctc
     6541 tatctggatg taatgaccgc actggcagaa gcctttaata atggcgagcg cgaaactctg
     6601 ccccgtgtca ttggtgggcg ctatggtctt tcatccaaag aatttggccc ggactgtgta
     6661 ctggcggtat ttgccgagct caacgcggct aaaccgaaag cgcgctttac ggttggtatt
     6721 tacgatgatg tgaccaatct gtcactgccg ttgccggaaa acaccctgcc aaactcggcg
     6781 aaactggaag ccttgtttta tggccttggt agtgatggca gcgtttccgc gaccaaaaac
     6841 aatatcaaga ttatcggtaa ttccacgccg tggtacgcac agggctattt tgtttacgac
     6901 tccaaaaagg cgggcggcct gacggtttct caccttcgcg tgagcgaaca gccgattcgt
     6961 tccgcttatc tcatttccca ggctgatttt gttggctgcc accagttgca gtttatcgat
     7021 aaatatcaga tggctgagcg tttaaaacct ggcggcattt tcctgctcaa cacgccgtac
     7081 agcgcagatg aagtgtggtc gcgcttgccg caggaagttc aggccgtgct aaatcagaaa
     7141 aaagcgcgct tctatgtgat taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc
     7201 cgtattaata ccgtcatgca gatggctttt ttccatctga cacaaattct gcctggcgat
     7261 agcgccctcg cagaattgca gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat
     7321 ctggtggaac gcaactggca ggctctggcg ctggcgcgtg aatccgtaga agaagttccg
     7381 ttgcaaccgg taaatccgca cagcgccaat cgaccgccag tggtttccga tgccgcccct
     7441 gctttcgtga aaaccgtaac cgctgcgatg ctcgccgggc ttggtgatgc cctccccgtt
     7501 tcggcgctgc cgccagacgg cacctggccg atgggcacca ctcgctggga aaaacgcaat
     7561 atcgccgaag agatccccat ctggaaagag gaactctgta cccaatgtaa ccactgcgtc
     7621 gccgcttgcc cacactcagc tattcgcgca aaagtggtac cgcctgaagc gatggaaaac
     7681 gcccctgcca gcctgcattc gctggatgtg aaatcgcgtg atatgcgcgg gcagaaatat
     7741 gtcttgcagg tggctccgga agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg
     7801 gcgaaagacc gtcagaatcc agagattaaa gccatcaata tgatgtcgcg cctggaacat
     7861 gtcgaagaag agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc
     7921 aaactggaac gtattgatat tcgcacatcg cagctgatta caccgctgtt tgaatattcc
     7981 ggtgcttgct ccggttgtgg cgagacgccg tatattaaat tgctgactca gctctatggc
     8041 gaccggatgt tgatcgctaa cgccaccggc tgttcttcaa tttatggcgg taacctgccc
     8101 tctacaccgt ataccaccga tgccaacggt cgtgggccgg catgggcgaa ctctctattt
     8161 gaagataatg ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc
     8221 gtgctgcgtc tgctggatca atttgccgat aaaatcccgg cggaattact gacggcgttg
     8281 aaatcagacg ccacgccaga ggttcgtcgt gaacaggttg cagctttacg ccagcaactc
     8341 aacgatgttg ccgaagcaca tgaactgctg cgtgacgcag atgcactggt ggaaaaatca
     8401 atctggctga ttggtggcga cggctgggct tacgatatcg gctttggcgg tctggatcat
     8461 gtattgagtt tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg ctattccaac
     8521 accggtggtc aggcgtcgaa agcgacaccg ctgggtgcag taactaaatt tggcgagcac
     8581 ggcaaacgta aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg tcatgtttat
     8641 gtggctcaga tttctctcgg cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg
     8701 gaagcgtatc cgggaccatc gctgatcatt gcttatagcc cgtgtgaaga gcatggttac
     8761 gatctggcac tcagccatga ccagatgcgc caactcacag ctaccggctt ctggccgcta
     8821 tatcgcttcg atccgcgtcg tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc
     8881 ccgccgtcag aagcactgga agaaacgtta cttcacgagc aacgtttccg tcgtctgaat
     8941 tcgcagcagc cagaagtggc agaacagtta tggaaagatg ctgcagctga tttgcaaaaa
     9001 cgctatgact tcctggcaca aatggccgga aaagcggaaa aaagcaacac cgattaaatg
     9061 ctctggataa ggattatcca attctaaaaa aaagcccgga cgactgttcg ggcttgtctt
     9121 tttatatgtt ggaaaatcag tggcaatgca atggcccaac agaaaataaa atctgtgaca
     9181 ataaaggcat ata
//
LOCUS       DAZFDC010000155         9170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFDC010000155 DAZFDC010000000
VERSION     DAZFDC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.155"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            28..390
                     /locus_tag="ACHJ48_004147"
     CDS             28..390
                     /locus_tag="ACHJ48_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709593.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMSKGREE"
     gene            complement(569..1027)
                     /gene="ybbJ"
                     /locus_tag="ACHJ48_004148"
     CDS             complement(569..1027)
                     /gene="ybbJ"
                     /locus_tag="ACHJ48_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO4709594.1"
                     /translation="MMELIVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1024..1941)
                     /gene="qmcA"
                     /locus_tag="ACHJ48_004149"
     CDS             complement(1024..1941)
                     /gene="qmcA"
                     /locus_tag="ACHJ48_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO4709595.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2087..2764
                     /gene="fetA"
                     /locus_tag="ACHJ48_004150"
     CDS             2087..2764
                     /gene="fetA"
                     /locus_tag="ACHJ48_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO4709596.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2751..3530
                     /gene="fetB"
                     /locus_tag="ACHJ48_004151"
     CDS             2751..3530
                     /gene="fetB"
                     /locus_tag="ACHJ48_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO4709597.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3593..4447)
                     /gene="cnoX"
                     /locus_tag="ACHJ48_004152"
     CDS             complement(3593..4447)
                     /gene="cnoX"
                     /locus_tag="ACHJ48_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO4709598.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4508..5317)
                     /gene="ybbO"
                     /locus_tag="ACHJ48_004153"
     CDS             complement(4508..5317)
                     /gene="ybbO"
                     /locus_tag="ACHJ48_004153"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO4709599.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRRSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5307..5930)
                     /gene="tesA"
                     /locus_tag="ACHJ48_004154"
     CDS             complement(5307..5930)
                     /gene="tesA"
                     /locus_tag="ACHJ48_004154"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO4709600.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFLMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5901..6587
                     /gene="ybbA"
                     /locus_tag="ACHJ48_004155"
     CDS             5901..6587
                     /gene="ybbA"
                     /locus_tag="ACHJ48_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO4709601.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6584..8998
                     /gene="ybbP"
                     /locus_tag="ACHJ48_004156"
     CDS             6584..8998
                     /gene="ybbP"
                     /locus_tag="ACHJ48_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO4709602.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNYVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVTLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2086 a         2363 c         2525 g         2196 t
ORIGIN      
        1 aggtttacgc caggcaggaa aatttaaatg aatgaatttg agaagatttt taatgaaatg
       61 aatctggacc gggcattgct accgatcctt tttcgttcga accggtcgac agtgtggaag
      121 tatttaagcg gcgatagtac ggctcctgcc tctgccatgt cgctgattat gctgttgcag
      181 ttgatacaga aacgaaaccc cgatctgtta gccgaatggt taacgctttc tgatttcacc
      241 attccgccag aagtttacct ggaccagcct gattactgga aagggtgggt gtatacgcaa
      301 cacaaggtca ataaaaacgt cctggaatat ctaaaaaaac actatccgga tgaagaccaa
      361 aaaagtatga gtaaaggccg ggaagagtaa attgccaatg ccagttaaca tttaacatgg
      421 cggaggcggc ctacggtttt ggcgtgatat gacgcgcccg cgtcgcatca ggcgtcggtg
      481 cacgactgcc ggatgcggcg taaacgcctt atccggccta cgattcgggc gcagacttcc
      541 gggcatgata agacgcgtca gcgtcgcatc aggcgatgac cgcacggatg atcagcgtta
      601 tcccttctat cgcaatgact tcaacatgcg tacctgcgcc gagatcctcg ctggcgctga
      661 caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc ggagattcca
      721 gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgactg tcgctgtgct
      781 tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca
      841 ggacggcaaa catcacccct tgccactccc aacccagcgg caccagccag accaccaggc
      901 cagtaatcac tgctgccacg ccactccaca acaaataacc atttccgccc agcatctcgg
      961 ctgccagcag caaaccgccg agactgagcc agaaaatatg tggatgaacg actattaact
     1021 ccatcatggc tgagtccgct tgttggcgct gtctttcacc agctcggcaa tcccggcaat
     1081 cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt tactggagga
     1141 accgatctgc tgtaacgctt cggtgtattt ctgcgctacg aagtagttca ccgcctgaat
     1201 atcaccggag gcgatggctt cagacaccat tttggtggcg cgggcttctg cttcagcgga
     1261 acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat
     1321 ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc
     1381 aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag
     1441 ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc cccacgggtt
     1501 ggtggcctca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat
     1561 ttcgtcaagt tccattgaac ccaacacggt acggatgtta gtcatggtca ggttgatgat
     1621 cgccagctcc agattgctga cttcataagc cgcgcgtggc gcgtcaatca cctgaataaa
     1681 acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat
     1741 atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac
     1801 cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta ccgtccattg
     1861 atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag
     1921 aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa
     1981 ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg
     2041 cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag
     2101 tcctttgctt cagctacaaa acgtaggata tctggcgggt gatgcgaaga ttcttaataa
     2161 tatcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg
     2221 caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact
     2281 gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgtcagc aagtctctta
     2341 ctgcgcccag acgccgacgc tgtttggcga tacggtatac gataatctga tctttccctg
     2401 gcagatccgt aaccagcagc ctgacccggc catttttctc gattttcttg aacgcttcgc
     2461 cttgccggac agcattttga cgaagaatat cgccgagcta tctggtggtg aaaaacaacg
     2521 catctcattg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg atgaaatcac
     2581 cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccatc gttatgtgcg
     2641 cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga
     2701 taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct atgaactcgc
     2761 ataatattac taacgaatca ttagcactgg cattaatgct ggtagtggtg gcaatcttaa
     2821 ttagccataa agaaaaactg gcgctggaga aagatattct ctggagcgtc gggcgcgcga
     2881 taattcagct gattattgtt ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca
     2941 gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac
     3001 gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg gtcggggcgg
     3061 gaattaccct ggcggtgctg attctctcag ggtcgattga atttatcccg atgcaggtga
     3121 tccctatcgc cgggatgatt gccggtaacg ccatggtagc ggtggggttg tgttacaaca
     3181 atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg agtcttggcg
     3241 cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc
     3301 cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg atgtccgggc
     3361 tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta
     3421 tgctgctctc aaccgccagc ttgtcgacca taattgcctg ctatttaacc tatcgtaagt
     3481 tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg
     3541 gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg atcaacggaa tatcaataca
     3601 acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag
     3661 cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca
     3721 gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctgatgc agttgcagcg
     3781 ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt tgcaactgtt
     3841 gaatttccgg cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc agcccctggt
     3901 agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac cgcttccgca tcttcagaac
     3961 ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ctccccgttc tgattcgaca
     4021 actgccaggc gtctttcagc aatggcaggg catcggtgta attgccttcc tgcatcagtt
     4081 gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc agcagggcgc
     4141 ggatcgcctc ttccggttgc ggcccctgga agccatctac cggttgcccg ttctggaaca
     4201 gatacacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc
     4261 agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcaaggctt tccagaattg
     4321 gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg
     4381 tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta
     4441 cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg
     4501 cttcaactca cccctgcaat attttgtcca tcacgcgccc cggcagcagg cgcttaagca
     4561 ccattaccgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa
     4621 aagcatggcg tactttgtcc accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc
     4681 cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg aagcgagtac
     4741 gaatgggacc gggttcgatc aggctgactt taattccgct gcggcgcagc tccatgcgca
     4801 gcgcgtctga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggtg
     4861 tggagattaa tcccatcacc gatgaggtca tcacaatacg cccttcaccg tgcggcaaca
     4921 tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct
     4981 gttccatctg cgcacggctg atggtggaaa gggggccata catgccgaat ccggcattgt
     5041 taaagatccc atacagacaa ttatcggtca gggcgatcac ctcgtcggct gcgcgatcaa
     5101 cactttctgg tgaatccaga tcgatcaaca cgccggtaaa tcccatgctg ttcatgcgct
     5161 caacatcatc cggtttccgg caacctgcca gcacatgaaa accctggcgt tttaattcga
     5221 gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa
     5281 ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaagg ctgcaactgc
     5341 ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc
     5401 tgcatccatt gtggcttgag gtagacctct tccattaaaa agggcagcag cggaacatca
     5461 aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag
     5521 tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc
     5581 aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa accgtcattg
     5641 ccgcccagtt caaccagcac ccaacgcggc tgatgctgtt tcagcagagc cggaaggcgc
     5701 gccagccctt gttgcgaggt gtcgccgctg atgctggcat tgactaccga cgttttactc
     5761 tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg atacccggcg
     5821 ctcaggctat cacccagaat caataacgtg tccgctgcgg cagcacggaa ggttaacagg
     5881 accagaaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat catcttaaga
     5941 agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag ctggttgtca
     6001 aacgtggcga gaccatcgca ctggtgggcg agtcgggatc gggtaagtca accttgctgg
     6061 cgatcctcgc cgggctggat gacggcagca gtggcgaagt gagtctggtg ggacaaccgc
     6121 tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt
     6181 ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag cttccggcgc
     6241 tgctgcgcgg tgagagtagc gcggaaagtc gtaacggagc gaaagcgttg ctcgaacagt
     6301 tagggctggg taaacgtctg gatcatcttc cggcacagct ttccggcggt gaacagcaac
     6361 gagtggcgct ggcacgagcc tttaacggtc gacctgatgt gctgtttgcc gacgaaccca
     6421 ccggtaacct tgaccgccag acgggcgata aaattgccga cctgctgttt tccctcaacc
     6481 gtgagcatgg caccacgttg attatggtga cccacgacct gcaactggcg gcacgctgcg
     6541 accgctgctt acggctggtg aacgggcagt tgcaggagga agcatgattg cacgctggtt
     6601 ctggcgcgaa tggcgctcgc cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt
     6661 ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaagggct taagccaaca
     6721 aagccgggag tttatggcgg gcgatcgggc gttgcgcagt tcgcgcgaag tgccgcaagc
     6781 gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga ctttcgccac
     6841 catgacattt gcaggcgaca caccgcagct ggcgaacgtt aaagcggtgg atgatatcta
     6901 cccgatgtat ggcgatctgc aaaccaatcc ccctggcctg aaaccgcagg cgggcagcgt
     6961 attgctggcc ccacgcctga tggcactgct taacctgaaa acgggcgaca ccattgacgt
     7021 gggcgatgcc accttgcgga ttgccggaga agtgattcag gaaccggatt ccggttttaa
     7081 ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata aaaccggagc
     7141 cgtgcagccg gggagtcggg tcacctggcg ctataaattc ggcggcagcg agaaccagct
     7201 cgacagctat gagaaatggt tgttacctca gcttaaaccc gaacaacgct ggtacggtct
     7261 ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt tcctgctgct
     7321 ttcggcactt ctgaccttgc tgctggcagt ggcagcagtg gcggtagcga tgaatcatta
     7381 ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca
     7441 actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag ccgttaccgg
     7501 tggggcgata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc
     7561 tgctgcacta ccgccagcca gcctctggcc atggctatgg gcgctgggca ccatgacggt
     7621 catctcgctg ctggtggggc tacgaccata ccgcttgttg ctggcaacgc agcctttacg
     7681 cgtattacgt aattatgtgg ttgcgaacgt ctggccgctg aagttttatc tgccgattgt
     7741 cagtgtggtg gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc tgctttgggc
     7801 ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt gtgctgggct ggatgctgct
     7861 gaatgtactt cgccgcatga cgctgaaatc gctgcctctg cgcctggcgg ttagccgcct
     7921 gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct cctttatgct
     7981 gctggcactg ctactggtgc tgcgtggcga tctgctcgac cgctggcagc agcagttacc
     8041 gccagaaagc ccgaactact ttttaatcaa catcgccaca gaacaggtta cgccgctaaa
     8101 agcgttcctc gcggaacatc agatagtccc ggaatcgttt tatccggtgg tgcgagcgcg
     8161 gctgacggcg attaacggca agccgacaga aggcaatgaa gatgaggcgc tcaaccgcga
     8221 actcaatctt acctggcaaa atacgcggcc cgatcataat ccgattgtcg ccggtaactg
     8281 gccgccaaaa gctgatgaag tttcgatgga agaggggctg gcgaaacgct taaacgttac
     8341 cctcggcgat accgtgactt ttatgggcga tacccaggag ttccgcgcta aagtgaccag
     8401 cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc tattttattt tccctgaagg
     8461 ggcattagac gggcaaccgc agagctggct taccagtttc cgctgggaga atggcaacgg
     8521 catgttgacg caactcaacc gccagttccc taccattagc ctgttagaca ttggcgcgat
     8581 tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg gcgctggagg tgatggtggt
     8641 gctggtcacc gcctgcggta tgttgctgtt gctggcacag gtgcaggtgg gaatgcgtca
     8701 gcgtcatcag gagctggtgg tgtggcgcac actcggtgcg gggaaaaaac tgctgcgtac
     8761 cacgttgtgg tgtgagttcg ccatgcttgg gtttgtttcc ggcctggtgg ccgcaattgg
     8821 tgcggaaacg gcactggcag tgttgcaggc gaaagtgttt gattttccgt gggagccaga
     8881 ctggcgattg tggattgttc tgccgtgcag cggtgcgctg ctgctgtcgc ttttcggcgg
     8941 ctggctgggc gcgagactgg ttaagggtaa ggcgctgttc aggcagtttg cggggtgatg
     9001 agagtaaata aggggcgttc tggttgaatg gaacgccttg tttaaattaa ttctacaata
     9061 tgtttcattg atgatgttat tgaattggtg atttcctatc tttctattgc tgatattaat
     9121 ctgaatcggt ggggtttaat aattttaaat atagccctac aaaaataact
//
LOCUS       DAZFDC010000156         9050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFDC010000156 DAZFDC010000000
VERSION     DAZFDC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.156"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            265..1014
                     /locus_tag="ACHJ48_004157"
     CDS             265..1014
                     /locus_tag="ACHJ48_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4709603.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
     gene            1011..2714
                     /gene="sctC"
                     /locus_tag="ACHJ48_004158"
     CDS             1011..2714
                     /gene="sctC"
                     /locus_tag="ACHJ48_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFO4709604.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGDGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            2719..3864
                     /locus_tag="ACHJ48_004159"
     CDS             2719..3864
                     /locus_tag="ACHJ48_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN/LcrE/InvE/MxiC type III secretion system
                     gatekeeper"
                     /protein_id="HFO4709605.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMRDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLNNALVTLPADANYKDKTLLLKAIYSGCTNLPFSLF
                     INNEQLLEIRECCKQAIKVTFAAELFDTQNCNKKQNKKPWKKVMFNV"
     gene            3857..5917
                     /locus_tag="ACHJ48_004160"
     CDS             3857..5917
                     /locus_tag="ACHJ48_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4709606.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIVVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQETKYILD
                     QLEMKYSDLLKEILRYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKDIIT
                     LVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEM
                     ILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNI
                     LKTI"
     gene            5949..7241
                     /gene="sctN"
                     /locus_tag="ACHJ48_004161"
     CDS             5949..7241
                     /gene="sctN"
                     /locus_tag="ACHJ48_004161"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFO4709607.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASRQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITEFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKNLTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            7231..7689
                     /locus_tag="ACHJ48_004162"
     CDS             7231..7689
                     /locus_tag="ACHJ48_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO4709608.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVTVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            7667..7900
                     /locus_tag="ACHJ48_004163"
     CDS             7667..7900
                     /locus_tag="ACHJ48_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709609.1"
                     /translation="MDEVKKIEHCKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDNQFSTLGKKNGHMKSIDNKSSL"
     gene            7973..8542
                     /locus_tag="ACHJ48_004164"
     CDS             7973..8542
                     /locus_tag="ACHJ48_004164"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFO4709610.1"
                     /translation="MTSRDQGINSELSNRTIQFKEKIHNGIHTEYITDQKHSNNKDRE
                     KKYRDGDKINGPQAHSLDITNERRFADNRTMFTQHIEKQRNVNTLNQNDINNSANNAN
                     VRENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSDRLHEAQHNDVTAQRWVLTE
                     QDERQGQRHQPHEEQENEGKFENDQKDES"
     gene            8502..>9050
                     /locus_tag="ACHJ48_004165"
     CDS             8502..>9050
                     /locus_tag="ACHJ48_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HFO4709611.1"
                     /translation="MKANSKTIRRMKVNVRSEESKSKHSTFETTFQNWKENGEDVALL
                     MPEFSAKWLPIAEESGSWSGWVLLREIFPLISAELAGMALMPETERLIGEWLSLSSSP
                     LNLKYPELKYNRLCVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYENAPTLDKK
                     SLYWPIHFVIGFSKTCYRTIVDI"
BASE COUNT         2970 a         1515 c         1970 g         2595 t
ORIGIN      
        1 agtgtgttaa tgaagaacta acaaatatga tttgtattga aaatagttac caaacaagtg
       61 ttataagtaa tacgaggtgt gtttaagtaa aacatatcaa cagttacaaa tctgtatatg
      121 gacaggatga cgtactagtg taagtggctg atagctattc gctcataaca aaatcattat
      181 tttataaata gtaatcatca ctttttaatc atgtttttat agcaagttta tggagaatga
      241 gaatatggaa taaggagatg aaacatgatt gaagaagggc tgttacttcc aatgaatgtt
      301 ttgcttcaag gacagaagtt aaccctactg gaccctgaaa tgtggttctt acggggaaat
      361 gaaaacaagg atgtaaccct tgttattacg attggtaata accatcaaaa attaatggta
      421 gtggaagata tattactgct cattgaccga agccaaattg aggtaactgc agggaaagta
      481 atttatcatc cacttcgcat tgatatcctg agtaaattat tagcttttat agatgaatca
      541 acggggagtg tggaaaggga acaccatgaa ttgtttgctg atgccttgcc ctttgcatca
      601 gaagttgtgc tattcagtaa agttgcaagt gaagcctggt ttttagccac ttatctgagc
      661 agcgataata ttaatgaaat cctgtttcag catctaagaa aaacagaatg ctataaactg
      721 gttcgttatt tattatcgca atcgctcata cagacttcac tctatgatct tggtgagctt
      781 tacggcgtat cgtattcaca ttttcgccgt ttatgtagtt atgcattggg tggtaaagtt
      841 aaaacggaat tatgtggctg gagagttgcc agagccgtgt tggaaatcat tgaaggaaac
      901 agtgatatga caactattgc gcataaatat ggttactcgt cctcatcaca cttttcagcc
      961 gaagtaaaaa gccggttagg aaaaacaccg agagaactat gtaaaaagtt atgaaaataa
     1021 aattacgcat tactattata ttaatttcag tcttatgcat ttttaatgga ttattgactc
     1081 ctggtgcata tgccgcagca gcgaatggat acgtagcaaa taaagaaaat ctacgtagtt
     1141 tttttgaaac ggtttcatca tatgcgggta agcctacaat agttagtaag cttgccatga
     1201 agaaacagat cagcggaaat tttgatttaa cagaacccta tgccttgatt gaacgcctgt
     1261 cggcacagat ggggcttatt tggtatgatg atggcaaagc tatttatatc tatgactcat
     1321 cagagatgcg taatgcattg attaatctga gaaaggtatc gacgaatgag tttaataatt
     1381 tcttaaaaaa atcgggtctg tataactctc gttatgaaat taaaggggac ggcaatggta
     1441 ctttctatgt atcggggcca ccggtttatg ttgacctggt cgttaatgct gcgaaattaa
     1501 tggagcaaaa cagcgatggc attgagatcg gtcgaaataa agtaggaata attcatctgg
     1561 tcaacacatt cgttaatgac cggacttatg agttacgtgg cgaaaagata gttattcctg
     1621 gcatggcaaa agtactatcg acgttgttaa ataataacat taagcaaagc acaggggtga
     1681 acgtactttc tgaaatttca agtcgtcaac aactaaaaaa cgtatcacgt atgcctcctt
     1741 ttcctggggc tgaagaagat gatgacctac aagtagagaa aataatatcg acagcaggag
     1801 cgccagagac tgacgatata caaattattg catatcctga taccaacagt ttacttgtta
     1861 aaggaacagt atctcaggtt gattttatcg agaagttagt agctacgctt gatattccaa
     1921 agcgacacat tgaattatct ttatggataa tagatattga taagactgat ctagaacagc
     1981 taggtgctga ctggtcgggc acgataaaaa ttggatccag cttaagtgca tcatttaata
     2041 attcaggttc aattagtacc cttgatggga cacagtttat tgcaacaata caggcacttg
     2101 cacaaaaaag aagagccgcc gttgttgctc gtcccgtcgt tttaacacaa gagaatattc
     2161 ctgctatatt tgataataac cgcactttct atactaaatt agtcggcgag cgtactgcgg
     2221 aattggatga agtaacctat ggtacgatga ttagcgtgct accacgattt gctgcgcgaa
     2281 atcaaattga attactcttg aatattgaag atggaaatga aatcaattcc gacaagacca
     2341 atgttgatga tctgcctcag gttggtagaa cacttataag tactattgca cgcgtgcccc
     2401 aagggaaaag tcttttgatt gggggatata cacgcgatac gaatacctat gaaagtcgca
     2461 agatcccaat attaggcagc attccattta ttggtaaatt atttggttat gaaggaacaa
     2521 atgcgaataa catcgttcgt gtctttctta ttgagccaag agagatcgat gaacgcatga
     2581 tgaataatgc gaatgaggct gcggttgatg ccagagcgat tacacagcaa atggcaaaaa
     2641 ataaagaaat caacgatgaa ttactgcaga aatggataaa aacttacctg aatcgtgaag
     2701 tcgtcggagg atagtgaaat ggcgattcat gtagagcatg tcggtgtatt agaaagagcc
     2761 agggaagttt ctcgcttaga ggacattatc acagaagata atgaagacat tgaagctgaa
     2821 atgcctaaaa tgagggatga tcccgcaggt aaagaagctc ggttcttaca agctactgac
     2881 gagatgtcag cagcgttaac gcagtttatg aagaaaaaaa tttacgaaga acaactggcc
     2941 aattttctgg acggagaaga atatgtcctg gaagaccagc caatagaaaa aaccgacaaa
     3001 gttatggagg cgcttaaggc tgcaactacc catgactatg aagtgtatag ctttgctaaa
     3061 aaattattcc cagatgaaag cgatctggta gttgtgttac gtgcaatttt acgtaaaaaa
     3121 caaatatcag aaaatgtccg gctaaacgct gaagcattac ttagaaaagt taatcaagag
     3181 actactaaaa aattcattaa tagcggaatt aactcggcat taaaagcaaa gttatttggc
     3241 caggcgctgt cattaaatcc aaaattacta agggcgagtt atcggcaatt tttaatggca
     3301 gaagacgatg ctgtagatac ttatgtcgaa tggattggta gttatggcta tcaaaataga
     3361 atgttggtga caaaatttat caaagagacg cttttctcag atataaatgc acttgatgcg
     3421 agttgttcat cattggaatt tggtatgttt ctcaataagt tgtcacaact attgtctctt
     3481 cagtcggcag aggctttatt tctgaaaact ttaatgaata acccaataat taaaaaattt
     3541 atcagcgcag aagattactg gatatttttt ctgatatcgt taataaaatt tcctgagact
     3601 gcagaggaac tcctgaacaa tgcactggtg acccttcccg cagatgctaa ttataaagac
     3661 aaaacgttac tcctgaaggc gatttacagt ggatgtacaa atcttccatt ttcacttttt
     3721 attaataatg aacaattatt agagattagg gagtgttgta aacaggcaat aaaagtaaca
     3781 tttgcagcag agctctttga tacacaaaac tgtaacaaaa aacaaaataa aaaaccatgg
     3841 aagaaggtga tgttcaatgt ttaacaaagt tttggtaggg ttaaggagtc atccagaatt
     3901 aataattctt ggattaatgg tgatgattat cgccatgctc ataattccat tgcccaccta
     3961 tttaatcgac tttttgattg ggcttaactt aacattggca atacttgtat ttttaggatc
     4021 attctatgtt gataggatct taagcttttc ttcatttcca tcaatcctcc ttatcacaac
     4081 gttatttcgt ttggcgctgg cgatcagtac cagtcgactt atacttcttg aggcggatgc
     4141 tggagaaatt ataacgagtt ttggtgagtt cgttattgga gatagcctgg tagttggttt
     4201 tgttattttc tccattgtta ctattgttca gtttatcgtg attactaaag gttcggaacg
     4261 tgtggctgaa gttgctgccc gcttttcgct tgacggtatg ccaggtaaac aaatgagcat
     4321 tgacgccgat ctacgcgccg ggatcattga tgcagattta gcaaaagaac gtcgtagtgt
     4381 attagaacga gagagccagt tgtatggttc ctttgatggt gcgatgaaat ttatcaaagg
     4441 tgatgctatt gcaaacatta ttattatctt tgttaatatt atcggtgggc tatcagtagg
     4501 agtaggccaa aatggaatgg atttctcaac agcgttgact gtctacacaa ttcttactgt
     4561 cggtgatggt cttgtttctc aaattccagc tttattaatt gctattagcg caggatttat
     4621 cgttacgcgc gtaaatggcg atagtgataa tatggggcaa aatataatgt cccagttatt
     4681 aagtaattct tttgtcattg ttgtgacatg tgtcctcgcg ctaagtattg ggttgcttcc
     4741 aggttttccc cttctagtgt ttttatgttt ggcagtaatc ttaggaattt acttttactt
     4801 taaattcaaa aagaaaggga ctgaagaaac ggttgttgag ggagatatta ccgttgggtt
     4861 ggaaccctat aatcaagatg atgatatctc attaggtatc attaataaac ttgatcaggt
     4921 tattaccgaa acggtaccct tggtcttgat tatgaatagt gtacaggcta aaaaatacac
     4981 tgaaatcaat cttgctgata ggattcgtag tcagtttttt attgagtatg gtatccgcat
     5041 tcccggtatt gtcattcgtg agggggaagg tcttaatgat gaagatgtca tattaatgct
     5101 aaatgaagtc agagcttcac aatttaaaat ttatcatgat cttgtcttgt tggttgaata
     5161 ttcagatgaa gtggtttcga ctttgatcaa gaaacctgtc attgtaaata gtaatggtga
     5221 acaatattat tgggttacga aaagtgatgc tcaaaaatta acaaaaattg gctgttatac
     5281 tcgcacggcc atggatgaaa tgtataatca tttatctgtc tgtctggctc ataatattaa
     5341 tgagtacttt ggtatacaag aaacaaaata tattcttgat caattagaga tgaagtattc
     5401 tgatctatta aaagaaatat taagatatat aaccgtgcag cgcatttcag aggtaattca
     5461 gcgtctgatc caggagcgaa tttcagtgcg caatatgcgg ttagttatgg aggccttagc
     5521 attatggagc ccacgagaga aagatataat cacactcgtc gagcatgtac gaggtgcatt
     5581 aggtcgctac atttgccata agttttcata ttctggagaa attaaagcaa ttgtgatttc
     5641 gcctgaaata gaggacagaa tcagagatgg tgtcaggcct accgctggcg gtacttttct
     5701 taatctcgat gcatccgaag ctgagatgat tctggataat tttaaactgg cactttctgg
     5761 aatcaatatt cctattaaag acattatttt actgggatct gtggatattc gtcgttttat
     5821 caaaaaactt attgaatcta gttatcgaga cctcgaagta ctttcgtacg gggaactaac
     5881 ggaaaatgtt cccgttaata ttctgaaaac tatttaggat aatgtcttgc gatatggagc
     5941 atgctggtat gaaaaaatta aaattattaa ataaatacag ctacttacat agcataaacg
     6001 gctctctgat tgaagcagaa cttgacgacg tttctgtggg tgaagtatgc gaaatttatg
     6061 cgagcaggca agcaaatgaa cgtatagcca gggctcaggt cgttggcttt cgcaatggga
     6121 agacattatt aaaccttata ggcagctctg tggggcttac acgtactgca gttttgaaac
     6181 caacgggaga gcagttaacc atacagatca gtgatgcctt tcttggttcg gttttgaatg
     6241 cctctggtca aattatggaa aggtttgtcc cggacactcc aggcgatagg ggaaatttac
     6301 gcctgattga tgaactgcca ccgtcgtatc aggaacgtcg agttattaat acaccacttg
     6361 agacacggat aagagtcatt gatggcgtac tgacatgtgg cattggtcaa cgtgttggga
     6421 ttttcgcctc ggctggctgt ggcaaaacgg ttttaatgca tatgctcgta aacaatactg
     6481 aagctgatgt gtttgttatt ggcttgattg gcgaacgtgg tagggaagtt acggaatgcg
     6541 cggaatcgct caaaaaatca gtaaatgcag cgaaatgcgt attggtctat gccacctctg
     6601 atttttcgtc agtagaccgg tgtaatgccg ccctgatggc gacaaccgtg gcagagtatt
     6661 ttcgagatcg aggaaagaga gttgtacttt ttatcgactc aatgacgcgg tatgcacgag
     6721 cactacgtga tatgaaactg gcagcgggtg aaccacctgc cagacgtggt tatcctgctt
     6781 ctgtttttga cagtttgcct cgcttactgg agaggccggg accaacacta aaaggaagta
     6841 ttaccgagtt ttataccgtg ttactggaag gggaagatga atcagaccca ctgggtgacg
     6901 aaattcgctc aatccttgat ggccatatct atctaagtcg caaactcgcg ggtcaaggtc
     6961 actatccggc aatagatgtt ctaaaaagtg tgagtcgagt atttggtcag gtcactgatg
     7021 aaaaacacag agataatgct gcacgggtaa ggaagaacct cacaactttg gaagatctac
     7081 aggtgtttat tgacttaggg gaatatcgcg cagggcaaaa tgctgaaaat gattttgcta
     7141 tgaatgcccg gcctaaactc acaaattggt tgaaacagtc agttaatgaa aaaatgccaa
     7201 tgagtgaaac gctaaaagag cttgaacgaa ttgttaagta aagttaatcg acttatcaga
     7261 agaacagcgc agagcctggc tgcatgtgaa gcatcattac aaaagttaaa tgcagaaaaa
     7321 gaaaagttag ccgaaaagga acgcctgtat gatatgcaac ttaaaaacct gcagtcactg
     7381 ttagatatga aggagttact cggcgaagtt gtatttcgcc aggatatatt ttactcgtta
     7441 cgtaaagtta cagtcattca gcagcagatc gctgagataa atcttgaaaa gcaaaaaata
     7501 gccgagcggc gaaaaatact taacaaggaa atagttcaac agcaagcaca aagaaaacac
     7561 tggtggctaa aaggagaaaa atacgaccga ctaaaaaagc gcatcaaaaa acaactttta
     7621 aatcagatgc tttatcaaga cgagcttgaa caggaggaaa aatataatgg acgaagtcaa
     7681 gaaaattgaa cattgtaaaa ttttaaacac agacacaccc gataattctg cttcggattt
     7741 agacagtttc ttaaagaaaa acaaaaaaaa taaagaaatg agtgagattg tcgctggaac
     7801 cattctccag ttattacgtc agactcagga taaccaattt tctacgttgg ggaaaaagaa
     7861 tgggcacatg aagtctattg acaacaaatc gtctctctag gttgacagga atccatctct
     7921 ttctttgcaa acaaaaggag ccgagcaatt ttctacaaag aaacttctca atatgacttc
     7981 acgagatcag ggtattaatt ctgaactatc taatcgaacg atacaattta aggaaaaaat
     8041 ccataatggt atccatacag aatacatcac agatcaaaaa cacagtaata ataaagatcg
     8101 tgaaaaaaaa taccgcgatg gagacaaaat aaatggaccg caggcccatt cattagacat
     8161 tactaacgaa agaaggtttg ctgataacag aaccatgttt actcaacata tcgaaaagca
     8221 aaggaatgtt aatacgctca atcagaatga tataaataat tctgctaata atgccaatgt
     8281 gcgcgaaaat gaattgacat atcagtttca gcgttgggga caaaatcata cggtccggat
     8341 tctggaaagt agtgaaggga tacgactaaa accatcagac acacttgtct ccgacagact
     8401 ccatgaagcc caacacaatg atgttacagc acaacgttgg gtattgactg agcaagatga
     8461 acgacaagga caacgacatc agcctcatga ggagcaagaa aatgaaggca aattcgaaaa
     8521 cgatcagaag gatgaaagtt aatgttcggt ctgaggaaag taaatcgaaa cactctacgt
     8581 ttgaaaccac ttttcaaaat tggaaagaaa atggcgaaga tgttgcatta ttaatgcccg
     8641 aatttagtgc taaatggcta ccgatagcag aagagagtgg cagctggtct ggatgggtgt
     8701 tgcttcgaga aatatttccc ttaatttctg ccgagctcgc tgggatggct ttaatgccag
     8761 aaacggaaag gttaataggg gaatggctca gtttatccag ttccccgtta aatctaaagt
     8821 atcctgaact aaaatataat cgtttgtgcg taggtaaagt atttgatgga gtattgagtc
     8881 cagctcaacc actgataaga atatggacag gggaattgaa tctttggtta gataaagtca
     8941 cagtctgcca atacgaaaac gctccaactt tagacaaaaa atcattatat tggcccattc
     9001 attttgtaat tggatttagt aaaacatgtt atagaactat tgttgatatt
//
LOCUS       DAZFDC010000157         9001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFDC010000157 DAZFDC010000000
VERSION     DAZFDC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.157"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            179..5607
                     /locus_tag="ACHJ48_004166"
                     /pseudo
     CDS             179..5607
                     /locus_tag="ACHJ48_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5723..6787
                     /gene="hipA"
                     /locus_tag="ACHJ48_004167"
                     /pseudo
     CDS             5723..6787
                     /gene="hipA"
                     /locus_tag="ACHJ48_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            7058..7324
                     /locus_tag="ACHJ48_004168"
                     /pseudo
     CDS             7058..7324
                     /locus_tag="ACHJ48_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7278..8546
                     /gene="ydeP"
                     /locus_tag="ACHJ48_004169"
                     /pseudo
     CDS             7278..8546
                     /gene="ydeP"
                     /locus_tag="ACHJ48_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
     gene            8794..8991
                     /gene="safA"
                     /locus_tag="ACHJ48_004170"
     CDS             8794..8991
                     /gene="safA"
                     /locus_tag="ACHJ48_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO4709612.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVILLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIVNSLQD"
BASE COUNT         2438 a         1943 c         2440 g         2180 t
ORIGIN      
        1 aagaactatg caatttttaa tctatcaatt cgaaagtatt agcaagttag ccaaattgac
       61 actttcccac ctccaactaa cactcaatac agacctctga caatttaata aatttcattt
      121 ttaatgtctg acgactcatc tctttcgtgg ctgtattgtt tgtatttgga gcgtttatat
      181 gaatagaatc tatcgcgtga tatggaattg cactctacag gtatttcagg cctgctcgga
      241 attaactcgc agggtaggta aaacatcaac ggttaatttg cgaaagtcct ctggactgac
      301 aacgaaattc agtagattga cgctgggtgt tttgctggca ctaagcggtt cagtgtctgg
      361 tgcgagtctg gaagttgata atggtcagat taccaatatt gatactgatg ttgcttatga
      421 tgcctacctg gttggctggt atggcactgg agtgcttaat attttggctg gcggtaatgc
      481 ctccttaacc actattacta ccagcgtcat tggcggtaat gaggattcgg agggtaccgt
      541 taatgttttg ggtggcacct ggcgattgta tgatagcgga aataatgcaa ggcctttaaa
      601 tgtgggtcaa tccggaacgg ggacgctgaa tattaaacag aagggtcacg tcgatggagg
      661 ctatttaaga ttaggtactc aagctgcagg cgtcgggacg gttaatgttg agggagagga
      721 ctcggttttg acgaccgaat tattcgaaat agggagttac ggtacgggct cattaaatat
      781 tacggataag ggttacgtca cgagttcaat agtcgccatt ttaggatatc aagcgaacag
      841 taatggtaag gttgtcgttg aaaagggtgg cgagtggcta ataaaaaata atgattcctc
      901 aattgaattt caaattggta atcaaggaac tggggaggcg actattcgcg agggtgggct
      961 gattacggct gaaaatacga ttatcggtgg caatgccacc ggtgtcggaa ccctgaatgt
     1021 gcaggatcaa gactctgtca tcacggtacg cagactctat aatggatatt tcggtaatgg
     1081 cgcagtcaat atttccaata atggactaat taataacaaa gaatattcat tggtgggcgt
     1141 tcaggacggt tcccacggtg tcgtcaacgt gaccgataaa gggcattgga atttcctcgg
     1201 aacgggcgaa gctttccgct atatctatat cggtgatgct ggcgacggtg aacttaatgt
     1261 ctcgcgtgaa ggtaaagtag attcgggaat tatcactgcg gggatgaaag aaacaggcac
     1321 aggcaacctt actgttaagg ataagaactc cgttatcact aatctcggaa ctaatcttgg
     1381 ttatgacggc cacggcgaaa tgaatatcag taatgagggg cttgttgtca gcaacggagg
     1441 aagctctctc ggttatggag aaaccggcgt cgggaaggtt agcatcacca cgggggggat
     1501 atgggaggtc aataagaatg tctataccac cattggtgtt gcgggcgtcg gaaacctcaa
     1561 tattagcgat ggcggtaagt tcgtatcgca aaatattact tttttgggcg ataaagcaag
     1621 cggtatcggc acactgaacc tgatggatgc gacatcgtcg ttcgatactg tgggtatcaa
     1681 tgtcggtaat tttggtagcg gtatcgtaaa tgtcagtaat ggtgccaccc ttaattcaac
     1741 gggctatgga tttatcggag gaaatgcctc cggtaaggga atagttaata tttcaacgga
     1801 tagtctctgg aatttaaaga cgtcttctac taacgcccaa ttgctacagg tcggtgtatt
     1861 aggcacgggt gaactgaata ttaccaccgg aggtatagtt aaagcgcgtg atacacagat
     1921 agctctcaat gacaaaagta agggcgacgt gagggtggat gggcagaact ctcttcttga
     1981 aacattcaat atgtacgtag ggacatctgg tacgggtacg ttaaccctga cgaatagcgg
     2041 cacgctgaat gtcgaaggtg gagaagttta cttaggtgtt tttgaacctg ctgtaggaac
     2101 gctaaacatt ggtgcagctc acggtgaggc ggcggcagat gccggattta tcaccaacgc
     2161 gacgaaagta gagtttggct ctggcgaagg tgtttttgtc tttaatcata ctaataacag
     2221 tgatgccggc taccaggtcg atatgctgat tacaggtgac gataaagacg gaaaagtgat
     2281 ccatgatgca ggccatacgg tgttcaatgc agggaatact tatagcggta aaacgctggt
     2341 caatgacggc ctcctgacca ttgcgtctca tacggcagat ggggtaacgg gcatggggtc
     2401 gagtgaagta accattgcaa gccccggtac gctcgacatt ctcgcatcaa cgaacagtgc
     2461 aggagattac acgctgacca atgcgctcaa aggcgatggc ttgatgcgag ttcagctgtc
     2521 atcctccgac aagatgtttg gctttacaca tgcaacaggg actgaattcg ccggtgttgc
     2581 ccaactgaaa gacagtacct tcactctgga acgcgacaac accgctgcgc ttactcacgc
     2641 gatgttgcag tctgacattg aaaataccac atcggtaaac gtgggagagc aatccattgg
     2701 tggactggcc atgaatggcg gtacgctcat tttcgatacg gatattcctg ctgcgacgct
     2761 tgcagaggga tatatcagcg tcgatacgct ggttgtcggc gcgagtgact acacctggaa
     2821 aggccgtaac tatcaggtaa acgggacggg cgacgtgctt atcggcgtgc ctaaaccgtg
     2881 gaatgatcct atggcgaata accctctgac gacgctcaat ttgctggaac acgatgataa
     2941 ccatgtcggc gttcaactgg tgaaggcgca aacggttatt gggtcgggtg gctcattaac
     3001 gttacgtgat ttacagggcg acgaggtgga agcggacaaa acgttacaca ttgcgcaaaa
     3061 cggaacggtg gtcgccgagg gtgattatgg attccgcctc acgaccgcac caggtgatgg
     3121 tttgtacgtt aactatgggc tgaaagcgct gaacatccat ggagggcaaa agctgacgtt
     3181 agccgaacat ggcggagcct atggcgcaac ggccgatatg tcggcaaaaa tcggtggtga
     3241 aggggatctg gcaatcaata cggtgcgaca ggtttcgctt tccaacggtc agaacgacta
     3301 tcagggggca acctacgttc agatggggac attacgtacc gatgcggatg gcgcgctggg
     3361 caacacccgg gaactgaaca tcagcaacgc agccatcgtc gatcttaatg gatcgacgca
     3421 gacggtagag acattcaccg ggcagatggg ttcgactgtt ttgttcaaag aagggtcgct
     3481 gacggtaaat aaaggtggga tcagtcaggg tgaactgaca ggtggcggaa acctgaatgt
     3541 tacaggggga acgctggctg tcgaggggct taatgcacgc tacaatgcgt taaccagcgt
     3601 tagcccaaat gcggaagtca gcctcgataa tacgcagggg ttaggcagag gaaatattgc
     3661 caatgacggt ctgttaacgc taaaaaacgt gactggcgaa ctgcgtaata gcataagcgg
     3721 gaagggtatc gtgagcgcaa ccgccaggac agatgtagag ctggatggcg ataatagccg
     3781 ctttgtgggg caattcaaca ttgatacagg cagcgcgctc agcgtcaacg agcagaaaaa
     3841 cctgggtgat gcttccgtta tcaataatgg cctgctcacc atctccactg agcgtagctg
     3901 ggcgatgacg cacagtatca gcggtagcgg tgatttgaca aaactgggta ccgggatcct
     3961 gactcttaac aacgattcct cggcgtatca gggtacgacg gatatcgtgg ggggggaaat
     4021 tgctttcggt tccgactctg ccattaatac ggcaagtcaa cacattaata tccataacag
     4081 cggtgtgatg tcgggaaatg tcaccactgc aggtgatgtg aacgttatgt ctggggggac
     4141 actgcgtgtc gctaaaacca caatcggcga atcggcggca acctggagaa tggcggcacg
     4201 gttcaaatga acagtgaagg aggaaaaccg gggaatgtac tgaccgttaa cggcaactat
     4261 accggaaaca atggcctgat gacgttcaac gcgacgctgg gcggcgataa ttcacccacc
     4321 gataagatga acgtgaaagg cgatacccaa gggaacactc gcgttcgggt tgataacatt
     4381 ggcggcgtcg gtgcgcaaac ggtcaacggt attgaactca ttgaggttgg cggtaattct
     4441 gcaggtaatt tcgcgctgac caccggaact gtcgaagctg gggcttacgt ctacacgctg
     4501 gctaaaggga aggggaatga cgagaaaaac tggtatctga ccagtaaatg ggacggcgta
     4561 acgccagcgg atacacccga tcccatcaat aatccccctg ttgtggatcc ggaaggccca
     4621 tcagtttatc gcccggaggc cggaagctat atcagcaaca ttgccgcagc caactcgctg
     4681 tttagccatc gcttacacga ccgtctgggt gagccgcaat atacagattc actgcattct
     4741 caggattcag caagcagtat gtggatgcgt catgtcgggg ggcacgaacg ttccagtgcc
     4801 ggagacggcc agctaaatac tcaggctaac cgctatgtat tgcagctagg cggcgatttg
     4861 gcgcagtgga gtagcaacgc gcaggatcgc tggcatcttg gcgtgatggc aggctacgcc
     4921 aatcagcaca gtaatactca gagtaatcgt gtgggttata aatcggatgg gcgcatcagc
     4981 ggttacagcg ctgggctgta cgcgacctgg tatcagaacg atgcgaataa gaccggcgct
     5041 tatgttgaca gctgggcgct gtataactgg tttgataaca gcgtcagttc cgataaccgt
     5101 tctgctgacg actatgattc tcgcggtgtg acggcctctg ttgagggtgg gtataccttt
     5161 gaagcgggaa catgtagcgg cagcgaaggg acgctgaata cctggtacgt ccagccacag
     5221 gcgcaaatca cctggatggg tgtgaaagat tctgaccatg cccggaaaga cggaacgcgc
     5281 attgaaacgg aaggcgacgg aaacgtgcaa acgcgacttg gggtgaaaac ctacctgaat
     5341 agccatcacc agcgtgacga tggtaaacag cgtgagttcc agccttacat tgaagcgaac
     5401 tggatcaaca atagcaaagt ctacgccgtg aagatgaatg gtcaaaccgt aagccgtgat
     5461 ggtgcgcgaa atctcggtga agtacgtacc ggggttgagg cgaaagtaaa taacaacctt
     5521 agcctgtggg ggaatgtcgg tgtgcaacta ggtgataaag gctatagcga tactcagggc
     5581 atgctgggag tgaaatatag ctggtaaaca gtataagccg catgtcgaga tggcatgcgg
     5641 cttaatattg ccaacttcaa aaggctttta aagcctccct tttatccgcg atcgcggata
     5701 tcgcagcgtt tatcccgtac agtttgattt attgtcagaa atagggcgag acagcgttgg
     5761 tgccgtgacg ttaatacccg aagacgaaac cgtaacgcgt ccgataatgg catgggaaaa
     5821 gcttactgaa gtcagacttg aagaagtatt aacggcttat aaagcagata tcccgctagg
     5881 catgattaga gaagaaaatg actttcgcat ctcggttgct ggcgcacagg agaagacagc
     5941 actgctcaga ataggcaatg actggtgcat tccgaaagga ataacgccga cgacgcacat
     6001 cattaaatta ccgattgggg aaatcaggca gcccaatgcg acgctcgatc tcagccaaag
     6061 cgttgataat gagtattact gtctgctgct ggcgaaagaa cttgggttga atgtcccgga
     6121 cgcagaaatc attaaagcgg gaaatgtgcg cgcgttagcg gtcgaacgtt ttgacaggcg
     6181 ttggaatgct gagcgaacgg ttttacttcg cttgccacag gaggatatgt gtcagacatt
     6241 cggtttacct tcatcggtga aatatgaatc agatggaggc ccaggcatcg cgcggatcat
     6301 ggcttttttg atggggttca gcgaggcgct tcgcgatcgt tatgatttta tgaaattcca
     6361 ggtcttccag tggttgattg gcgcaacgga tggccatgca aaaaacttct ccgtatttat
     6421 tcaggctggc ggcagttatc gactcacgcc attttacgac atcatttcag catttccggt
     6481 ccttggcggt acgggaatac acatcagcga tctcaaactg gcaatggggc ttaacgcatc
     6541 caaaggcaaa aaaacggcaa tcgataaaat ttatccgcga cattttttgg cgacagcaaa
     6601 ggtgctgaga ttcccggaag tgcagatgca tgaaatcctg agtgactttg ccagaatgat
     6661 tccggcagca ctggataacg tgaagacttc attaccgaca gattttccag agaacgtggt
     6721 gacggcagtt gaaaccaatg tgttgaggtt gcacggtcgg ttaagccgag aatacggtat
     6781 taagtaagag taagatatgg ggtttggtcg ttgttaatga gcaggacaat catgaccgcc
     6841 aacgactaga ttctcccttg gtttatattg gtgcgcataa tgtatattat gttaaatcgt
     6901 atctgagtga tacgaagtga ttttccggta gtcgttattc gttcaggatt ccagaactta
     6961 agcttctcct ctttcataat tggcctgtgc atgacggata taggctggct ggtatgccga
     7021 tttgccgttt gccgcatctt cccagcgttg aagactcaag ctggcaaacc gcgtgcaaat
     7081 gagcacgccc tctttccatc aacacttcag gcagcttgcg ggaatgagtc cactccggta
     7141 tcagaaatgg ctacgtctca atgaagtcag gcgaacgatg ctgaatgaac attacgatgt
     7201 caccacagca gcctatgctg tcggttacga aagcttatcc catttccgac gggaatattt
     7261 gcggatgttt ggagagcgaa cgtatttctg gactaagtca gacacaaatc gccgaactgg
     7321 ctgatgcata tgccgctgcc gaacgaacca ttatctgtta cggaatgggg atcactcagc
     7381 acgaacatgg tacccagaat gtacagcaac tggtcaatct gctgttgatg aaaggtaaca
     7441 ttggcaagcc tggtgcgggt atctgcccac tacgtggcca ctctaatgta cagggcgacc
     7501 gaaccgtcgg tatcaccgag aaaccgtctg tagagtttct ggctcgcctg ggtgagcgct
     7561 atggcttcac cccacctcat gtacctggac atgctgcaat tgccagcatg caagcaatat
     7621 gtacggggca ggctcgagca ttgatctgca tggggggcaa ctttgcactg gcaatgccag
     7681 atcgggaagc gagcgctgta ccgttaacgc aattagattt ggcggtacac gtagccacta
     7741 agcttaaccg ctctcatctg ctgaccgcac ggcatagcta tattctgccg gttctgggac
     7801 gtagcgagat tgacatgcaa aaaagcggtg cgcaggcggt aaccgttgag gattcaatgt
     7861 cgatgattca tgcctcgcgt ggcgtgttaa aacccgccgg tgtaatgctg aaatcagagt
     7921 gtgctgtggt cgcgggaatc gcgcaggcaa cactacccca gagcgtggta gcctgggagt
     7981 atctggtgga agattatgat cgcattcgca atgacattga agctgtgctg ccagagttcg
     8041 ccgactataa ccaacgcatc cgtcatcccg gtggttttca cctgataaat gcagctgctg
     8101 aaaggcgctg gatgacgccg tcaggtaagg ctaatttcat taccagcaaa gggctgttag
     8161 aagatccctc ttcagcgttt aacagtaagc tggtcatggc gacagtacgc agccacgatc
     8221 agtacaacac gacgatttat ggtatggatg atcgctatcg aggggtattc ggtcaacgag
     8281 atgtggtctt tatgagtgct aaacaagcta aaatttgccg tgtaaaaaac ggcgaaagag
     8341 ttaatcttat tgcgcttacg ccagacggta agcgcagctc acgccgcatg gatagattaa
     8401 aagtggtcat ttaccctatg gctgaccgct cactggtgac ctattttcca gaatcgaatc
     8461 acatgctaac acttgataac cacgatccat taagtggcat tcctggctat aaaagtattc
     8521 cggttgaatt agaaccatca aattaatgcc tcttctcatt tcttctgctg tcatccgcac
     8581 agcagaagaa ttcctcattg attattatcg cgcaatttgc tcacatggat taaattaaac
     8641 tacatactat aagatataaa cttctgccta cagctgtaag aaactccgct cagtactgaa
     8701 gcaccagtcc tatttcctct tttctcaagc ctgttatatt aagcatactg attaacgatt
     8761 tttaacgtta tccgctaaat aaacatattt gaaatgcatg cgaccacagt gaaaaacaaa
     8821 atcacgcaaa gagacaacta taaagaaatc atgtctgtaa ttgtggttat cttattactg
     8881 acccttacgt tgatagccat tttttcggca attgatcagc tgggtatttc agaaatgggt
     8941 cgcattgcaa gagatcttac acatttcatt gtcaatagtt tgcaagactg aaaataaaca
     9001 g
//
LOCUS       DAZFDC010000158         8945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFDC010000158 DAZFDC010000000
VERSION     DAZFDC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.158"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..812
                     /locus_tag="ACHJ48_004171"
     CDS             <1..812
                     /locus_tag="ACHJ48_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4709613.1"
                     /translation="YSIEKITAPVAINALDAVSDKNALQKKLISRLNEIMNYAVNCGA
                     LKANPLLKIKTAFTGKKNKSLAALPVERLPEFLSWWDSVPHTYQIAHNALLFQILTMV
                     RPGEAIKAEWSEIDFDSGLWIIPAHKMKCHREHVVPLSSQAISILRTMQEIKRGRYVF
                     FSSRTKDAPMGRNTIKTPIAASKFKGIVTLHGFRSMWSTLLNEEGFNPDVIEAALAHK
                     SGDKIRDIYNRTIYLEQRKIMMQWVGDFFDDARKGVINRSGGMKGLRVVNG"
     gene            826..1029
                     /locus_tag="ACHJ48_004172"
     CDS             826..1029
                     /locus_tag="ACHJ48_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4709614.1"
                     /translation="MNTNEDILFTKDVMKILRYGAMSAFINFWKDENNGFPQPFRIGR
                     RHTWHRRDVEAWLDKQREQANPH"
     gene            1089..1670
                     /locus_tag="ACHJ48_004173"
     CDS             1089..1670
                     /locus_tag="ACHJ48_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO4709615.1"
                     /translation="MNKNIAVTGKGDARHVKKFCDFRDLVVLRFDSVNVRVVYLNGDP
                     WFVAKDVCVALEISNSRDALKALDADEKKTVALSYGIRGNPNHSLISESGFYKLIARS
                     RKAVTPGTFAHRFSNWVFRNVIPGIRKTGAYGIPWGALQDFSRRKEQYQLSASQKGRE
                     LQACKRKKRELEEEEKRLIREYQPEFYFGNRIQ"
     gene            complement(1791..1970)
                     /locus_tag="ACHJ48_004174"
     CDS             complement(1791..1970)
                     /locus_tag="ACHJ48_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4709616.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKEAE"
     gene            2167..2370
                     /locus_tag="ACHJ48_004175"
     CDS             2167..2370
                     /locus_tag="ACHJ48_004175"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4709617.1"
                     /translation="MRNHTTHPQGRDLHNLNKFTWRFIALSTAQPRVITIEATSEQEA
                     RQQSPVGCVMVFAARIRQGVEHV"
     gene            2363..2587
                     /locus_tag="ACHJ48_004176"
     CDS             2363..2587
                     /locus_tag="ACHJ48_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709618.1"
                     /translation="MCNATWPDAAVDAIKTLMDSLIEISAIAGVAHKHAARESECIFH
                     YLAFVQLKADQALDKAGKIIMADVQEVHHA"
     gene            2580..3173
                     /locus_tag="ACHJ48_004177"
     CDS             2580..3173
                     /locus_tag="ACHJ48_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709619.1"
                     /translation="MHNLSISDLNSIQFDEIFSGQLLVNVENGRVVSNYHLPDGAIAG
                     SVEALLELAERARLIKPSTCHHDDDLHFTGRMVSHYENGVEVSRERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGGVNMRDDRFNSLKREFDGAPEDAAGALLSVADMMKAAYFLINT
                     SGYKSEGEMILSIASDYAEYVAETRYRRKFTEDVSHA"
     gene            3166..3365
                     /locus_tag="ACHJ48_004178"
                     /pseudo
     CDS             3166..3365
                     /locus_tag="ACHJ48_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3370..3612
                     /locus_tag="ACHJ48_004179"
     CDS             3370..3612
                     /locus_tag="ACHJ48_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4709620.1"
                     /translation="MKKKNSGFTASGLSRPEIRLGDIYRDTRRGGRVVIRHVTPGNIT
                     YRRDFTLVDHYKAVNQKRAASYIRKIREMLVAGGKK"
     gene            3609..5423
                     /locus_tag="ACHJ48_004180"
     CDS             3609..5423
                     /locus_tag="ACHJ48_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO4709621.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEHDARMA
                     GDNEPPVVLADEQLKEIGNLQIVPDGRTSARIFRAGQLDPVMVKAIGQKLAAASIQNA
                     NYYPDGMHSQKCENWREYLARERKNIDDGLIIRFPAKKKTEDSATPLALNQMGASQRG
                     EVLLAHYGGELAINADSDTVHHYNGVVWEPVQDKELQRTMAQIFIDAEISYSQNAIKS
                     AVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEGE
                     TLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFLEVTGPGGSGKSVM
                     AEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGGD
                     KVAIDPKHKAPYSTRIQAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDPM
                     LAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYLMS
                     SVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYGR
                     EYMKRKTKHGLRSNVILTEDSEDWMPSCASVTNDDGKN"
     gene            5711..5956
                     /locus_tag="ACHJ48_004181"
     CDS             5711..5956
                     /locus_tag="ACHJ48_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709622.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELV
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            5953..6375
                     /locus_tag="ACHJ48_004182"
     CDS             5953..6375
                     /locus_tag="ACHJ48_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO4709623.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATMWLSVLAFGRQADALAKHRKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            7033..8922
                     /locus_tag="ACHJ48_004183"
     CDS             7033..8922
                     /locus_tag="ACHJ48_004183"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO4709624.1"
                     /translation="MKTWFSIKAMADVVHVRIYDEIGGYGVKASALTDEINACGNVSE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVMPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITAMMEAETWMDG
                     NECKANGFADEVIAAITAMARIESKRIGDFSNMPEKIKSMISQKTTSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYGTRSQIVNA
                     AFTHSSSDFTDILAGGAEKSVLAGWEHSGETFRQWTKKGSLSNFREARRVGMNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLDQLSTVPMAMGRAASR
                     TVGNLVNLVLTGNVKLSDGIALFDKKHSNLIEAGLTTPGLSAARHLMRTQKDKNGEVL
                     NIAPKFLLVPAALEDRALQMINSTAPFGADKNSGIFNPYHKLLDIIVDPRLDDISEKQ
                     WYMLSAQGTDTIEVAYLDGSDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVKSG
                     GTDSL"
BASE COUNT         2592 a         1903 c         2349 g         2101 t
ORIGIN      
        1 agtacagtat agaaaaaatt acagcaccag tagctattaa tgctctggat gccgtatccg
       61 ataagaatgc gttgcaaaaa aaattaatat caagattaaa tgaaattatg aattatgctg
      121 taaattgtgg agcattgaaa gcaaatccat tacttaagat aaagactgca ttcacaggaa
      181 agaaaaataa atcattagca gcactacctg ttgaaagatt gcctgaattt ctgagctggt
      241 gggatagtgt gcctcatacc tatcaaatag ctcataatgc acttttattc cagatattga
      301 caatggtcag gccaggtgag gcgattaaag cagagtggtc agagattgat tttgattctg
      361 gcttgtggat tatccccgcg cataaaatga aatgccatcg tgaacatgtt gttcccttgt
      421 catcacaggc tattagtatc ctcagaacaa tgcaggaaat aaaaagaggg cgttatgtgt
      481 ttttttcctc cagaacaaaa gatgcgccta tggggaggaa tactatcaag accccaattg
      541 ctgccagtaa gttcaaaggg attgtaacgt tacatggttt tcgttcaatg tggagtacgc
      601 ttttaaatga ggagggattt aaccccgatg taatcgaggc tgcattggcg cataaaagtg
      661 gtgataaaat aagagatatt tataatagaa ctatttatct agaacagcgt aagatcatga
      721 tgcaatgggt cggtgatttt tttgatgatg cgagaaaagg ggtaattaat agatccggtg
      781 gtatgaaagg tttaagagta gtaaatggtt gaggaggttc agcaaatgaa taccaatgaa
      841 gatattttat ttactaaaga cgtaatgaaa atcttgcgat atggagcaat gagtgcattc
      901 atcaatttct ggaaagatga gaataatggt tttcctcagc cgttcagaat tggacgacga
      961 catacctggc accgtagaga tgtagaagca tggttagata aacaacgaga acaggccaat
     1021 ccccactaat aatatctttc ataccccgcg tgcaatgcgg ggttttttgt atgtgaggta
     1081 aaaaagaaat gaataaaaat attgccgtga cgggcaaggg tgacgcacgt catgtaaaaa
     1141 aattctgtga ttttcgtgat ctggtcgttc tgcgctttga tagtgtgaac gttcgcgtgg
     1201 tgtatctgaa cggcgatccg tggtttgttg caaaggatgt ttgtgttgct ctggaaataa
     1261 gtaactcacg cgatgctcta aaagcactag atgcggatga aaagaaaacc gtcgctttaa
     1321 gctacggtat tcgaggaaat ccaaatcata gcctcatttc tgaatccggt ttttacaagc
     1381 tgatcgccag aagtcggaaa gctgttactc ctggcacgtt cgcccatcgt ttcagtaact
     1441 gggtattcag gaatgtgata ccaggaatca gaaaaacggg ggcttatggt atcccgtggg
     1501 gcgcattaca ggacttttct cgccgtaaag agcaatatca gttaagtgcc agtcagaagg
     1561 ggagggagct acaggcatgt aagcgcaaaa agcgtgagct ggaggaagaa gaaaaaaggc
     1621 tgatacgtga atatcagcct gagttttact ttggcaaccg cattcagtga caaaacaaag
     1681 gcgacccgga tcagaggtcg ccaatgggaa aacactaaac ataagcccaa acatcatagc
     1741 gatttgttgg ctggtgggca atgtgatcag tgtgctggct tgctggtgga ctattccgct
     1801 tctttggcct tgcggcgctg gcggcgtttg atctcgcctc gcatggctgt aacgataaat
     1861 tgcgcagtgc tctcgcctgt ttcttttact tgctccatag cctcaacaac ttcatgcggg
     1921 gcgcgagcct gtaatttctg tgatttgtta ttgatatggt ctctttccat ttctggttcc
     1981 ctttgtgatt actggaatcc agtatacacg aaaaagacca aaacaaaacg cttgaagtgg
     2041 attccattta gtgattatac tggaatccag ttgagtgttt atcactcaat gcaaacgagg
     2101 ctgaaaagga ttgccgtcct ttcgcagcct ctgaccacca acgatagcaa cagtatcgag
     2161 gtagctatga gaaatcatac cacacacccg caagggcggg acttgcacaa cctgaataaa
     2221 ttcacctggc gttttatcgc cctgagcacg gcacaaccgc gcgtgattac cattgaggcc
     2281 accagcgaac aggaagcacg ccagcaatcc ccagttggct gcgtgatggt attcgccgcc
     2341 cgtattcgtc agggggtgga acatgtgtaa tgcaacatgg cctgatgcag cggtagacgc
     2401 tatcaaaacg ctgatggatt cacttattga gatttctgct atcgctggtg tggcgcataa
     2461 acacgcagcc agagaatcag aatgcatctt ccattattta gcatttgtgc agctaaaagc
     2521 cgatcaggca ctggataagg ccggaaaaat tatcatggct gatgtgcagg aggtgcacca
     2581 tgcataacct gtcaatttct gaccttaaca gcattcagtt tgacgagata ttttccgggc
     2641 agctactggt caacgtggag aatggacgag tggtaagtaa ttatcacctg ccggatggtg
     2701 caattgccgg aagcgttgaa gcattgctgg aactggcgga acgtgcgcga ctgattaagc
     2761 cgtcaacgtg ccatcacgat gatgatctgc attttaccgg acgcatggtg agtcactacg
     2821 aaaacggcgt tgaagtatcc cgcgaacgtc tgcgtgatga ttgctgtttc ggcacactgc
     2881 cggaatttat cgaattgctg accagttgcg gttatcaggt cattcagggg ggggtaaaca
     2941 tgcgtgatga tcgttttaat tccctgaaac gggaatttga tggcgcaccg gaagatgcag
     3001 cgggcgcatt gttgagcgtt gctgacatga tgaaagctgc atattttctt atcaatacca
     3061 gtggctacaa gtcagagggt gaaatgattc ttagtattgc gtcggactat gcggaatatg
     3121 tggcagagac gcgttacaga agaaaattca cggaggatgt aagccatgca taatcatgaa
     3181 gcgcatgtac ccgtagtgct taatgtgcca gatgatttca ccggacgcgt actggtttac
     3241 ctggataaag ggaaagtgaa atcacaatgc cgactgaaaa gtaatgagat tgttggttct
     3301 cctgaatttt ttctgaactc tgtattcgtg cggaaataaa accggaactg ctgacaggaa
     3361 aataaaacta tgaaaaagaa aaattctggc tttactgcca gcggtctctc tcggcctgaa
     3421 atcagactcg gagatattta ccgcgacacc agacgtgggg gacgagtggt tattcgtcac
     3481 gttacgccag gcaatatcac ctaccgccgt gattttacgc tggtggacca ttataaagcc
     3541 gtaaatcaaa aacgtgcagc aagctatatc cgcaaaatcc gtgaaatgct ggttgcaggg
     3601 gggaaaaaat gaaactggca ccgaacgtaa aaaaacagcc acgcggaata aaacacaaag
     3661 acacagaggt aattattttc gcgggtagtg atgcctgggc acacgcgaaa caatggcagg
     3721 aacacgatgc gcgtatggct ggagataatg agccacctgt tgtgctggct gatgaacaac
     3781 tgaaagagat cggaaatctg caaatagtgc ctgatgggcg cacatccgcg cgcatattca
     3841 gagccggaca gttagatcct gtaatggtta aggcaattgg tcagaagctg gcggcggcaa
     3901 gtatacagaa tgcgaattat taccctgatg gcatgcacag ccagaagtgt gagaactggc
     3961 gtgaatacct tgcccgtgag cgtaaaaaca ttgatgacgg actgattatc cggtttccgg
     4021 caaagaaaaa aacagaagac agcgcgacac cactggcact taaccagatg ggagcaagcc
     4081 agcgcggcga agttctcctg gcacattatg gcggtgaact ggcaatcaat gcggattctg
     4141 acaccgttca ccactacaac ggtgttgtat gggagccggt acaggataaa gagttacagc
     4201 ggactatggc gcagattttt attgatgcgg aaatcagcta ttcgcagaac gccatcaaat
     4261 cggcggtcga taccatgaag ttaagtttgc ccgtaatggg gaatacagcc cgtaacctga
     4321 ttggattcag taacggggta tttgatacca gaacaggtaa ttttcgggag cataacaaaa
     4381 acgactggtt gttaattgcc agtgaattac ctttcagccc accagcagag ggggaaacgc
     4441 tggcaacaca tgcgccgaat ttctggaagt ggttgcgccg ttcggtggct gagaatgacc
     4501 gcaaggcaga tcgcgtactg gcggcgttat tcatggtgct ggcgaatcgg tacgactggc
     4561 agttatttct tgaggtaacg gggccgggtg gaagcggtaa aagcgtaatg gcggagattt
     4621 gcactatgct ggcgggtaag gccaacacgg tatcggcaag catgaaggcg ctggaagatg
     4681 ccagggaacg tgcgttagtg gttggctttt cgctgattat catgccggat atgacccgtt
     4741 acgctggtga tggtgcaggg attaaggcca ttacaggcgg tgataaggtg gcaatcgacc
     4801 cgaaacataa agccccctac tcaacacgca ttcaggcggt cgtgctggcg gtgaacaata
     4861 acgccatgtc attcagtgac cgcagcggtg ggatatcacg tcgtcgggta atattcaatt
     4921 tttcggaagt tgtaccggag aacgaacgtg accctatgct ggctgaaaaa atagaaggag
     4981 agctggcggt agtgattcgc cacctgctta cacgcttttc tgaccaggac gaagctaaac
     5041 gcctgctata tgagcagcag aaatcagaag aagccctggt gataaaacgc gagggcgatt
     5101 cgctggtgga cttctgcggc tatctcatgt cgtcggtaat gtgcgatggc ctgttagtgg
     5161 gtaatgctga aattattccg ttcagcccgc gcaggtatct ctatcacgcc tatctggcgt
     5221 atatgagggc acacggattc ggtaagcccg taacactgac acgcttcggt aaggatatgc
     5281 caggggcaat ggcggaatac ggcagggagt atatgaaacg gaaaacgaag catggcttgc
     5341 gttcaaacgt gatactgaca gaggattcag aagactggat gccctcatgt gcatcggtca
     5401 caaatgacga tggcaaaaat taaacttatg gaataactgt tcatcactgt tcaccctgtc
     5461 ataaatatct tttatatcag tatattatat ggtgaacagt tatttatgaa ctgttcacca
     5521 aactattcac tgttcacctt tttgattgtt tattgagctt caagggtgaa cagtggtgaa
     5581 cagttggtga atagtttttg tgaaactgtt caccccttaa cattatgaat taaaagataa
     5641 aatatcaaaa ggtgaacagg tgaagggttg aaacgcaaaa attttaattt actgctgtga
     5701 gataaagcct atgacagcga agcacacaaa aaaatcacaa tcgcacgccc ttgatttgac
     5761 ggaacactgg ttaagggtgt cgataaaaat catcgaccgc aacgccgggg aaggatatgc
     5821 gaaagcacat cccgaactgg ttagcgcatt catgacaacg gcagctgcaa actttgccac
     5881 gctgacagaa cgggagattg ccgaagcgga acaggtaaca accatcaacg ttaaaaccgg
     5941 agaggtggaa tcatgacagc acagatagcg gcttacgggc ggctggtggc tgacccacag
     6001 ttaaagacca ccagcaaggg cacacaaatg acgatggcga gtatggctgt ccccctgccg
     6061 tgcagccagg cagatgacgg aacggctacg atgtggttat ccgtcctggc gtttggcaga
     6121 caggccgacg cactggcaaa acaccgcaaa ggcgagctgg tgagcgtggc gggtaacatg
     6181 caggtaagcc agtggacagg ccagaacggc gaaacgcggc agggctggca ggttatcgca
     6241 gacagcgtaa tcagtgcgag aacggcgcga ccgggcggca aaaaaggcca gcaggggcag
     6301 gctactgacg cactgaacag ggcaaaacaa caggcgggga atgatgatcc gtacggggat
     6361 aacataccgt tttaagcaac gagtgacaga agccggagaa atccggcttt tttgtaggta
     6421 ctcctggtgg gggtggcctg tccacggggc ggaggggcgc ggaaaaaggc gcattttttg
     6481 atttttatgg caccatcacc accactataa gctattgata tgttgagaaa taaaaatttt
     6541 tagtgtcgaa tcaggttgtt ttttgttcat caccggaacg ttcccgaaaa catttacaaa
     6601 aaacaggcgc aaaaaaagcg cccccgattg ctgttaccgg aggcgctttt acacgacaaa
     6661 ggagttttta tcgccaggat gacgagtctt aatactgctt caatggcaaa aatgcgtcaa
     6721 taactttgcc tttctgagaa taatcagaaa aaacataatc tgattttcag gtagaaaatg
     6781 atttatctat tacttttatc gatcaataat gatgcccgtt aatcaaaacg gaggcggatt
     6841 tatgccagag aacaacacca gaaagccgga taaaagtgcc acggtacaca tagacgccgg
     6901 aactatggag aagatcgaac gctatcagca gttcatcaaa gataatcacc cgggtatgcc
     6961 agtgcccacg aaaggacaaa tcacacgcag cgcggttgaa tactggtaca gggcaacgtt
     7021 aggagcctgg ctatgaaaac atggttttcc attaaggcta tggcagatgt tgtacatgtg
     7081 cgcatttatg acgagatcgg cgggtacggt gtaaaggcat cggcacttac tgacgagatc
     7141 aacgcgtgcg gtaatgtgtc tgaaatccat cttcgcatcc attcacctgg tggcgacatc
     7201 tttgaagggc tggctatcta taacgccctg aaaaatcatc cggcaaagaa aattgtacac
     7261 attgaaggca tggcggcttc tatggcctcg tttattgcca tgtgtggcga tcacatcgtt
     7321 atgcctgaaa acgcgatgat gatgatacat gccccccgtg gtgttactgc cggagtgtcg
     7381 ggcgacgttc gccgctttgc tgacctgatg gacaagctgg gcgacacgat ggcggaaacc
     7441 tacgccggaa gaacgggcag gagcaaacag gaaatcaccg ccatgatgga ggcggaaacc
     7501 tggatggatg gcaatgagtg taaggctaac ggcttcgcag atgaggttat agccgcgatt
     7561 acagcaatgg cccgaattga atcaaaacga atcggagatt tttcaaatat gccggaaaaa
     7621 attaaaagca tgatcagcca gaaaactacc agtggcgaac aggaacgact taacggcatc
     7681 cgtgaattgt ttggcacgtt caacggaaga tataacgacc tggctataag ttgtcttgct
     7741 gattcagaat gtagcgttga gaatgcacgc gaacgccttt tactcgctat gggtaaagaa
     7801 tcaacgccaa caaacaaaac cacccccgca aatctttact acgcgtacac ggataacggc
     7861 aacataaccg gcgatgcaat gcgccagggg cttaatgcgc gtcttggtca cgaacgggcc
     7921 gaacgcggta atccttacgc catgatgagc cttttcgata tggcacaggc atcattaacc
     7981 catcgtggta taagcacggg cagctacggc acacgctcgc agatagtaaa cgcggcattc
     8041 acccacagca gcagcgattt taccgatatt cttgctggtg gcgctgaaaa atcagtgctt
     8101 gcaggctggg agcacagcgg cgaaacattc cgccagtgga cgaaaaaagg ttccctttca
     8161 aacttccggg aagcccgccg cgttggtatg aatggcttct caacgttaaa caaagtgccg
     8221 gaaggggcag aatataaata catcaccacc agcgatcgcg gtgaacccat cgcgctggct
     8281 acttacggga atattttcag cattacccgc caggcgataa tcaatgatga ccttgatcag
     8341 ttatcaacgg tgccaatggc tatgggccgt gctgcatcaa gaacggtggg aaatctggtt
     8401 aatctggtgc ttacaggcaa cgtaaaactt tctgacggaa tagcgctgtt tgacaaaaaa
     8461 cacagcaacc tgattgaagc aggactgaca acaccgggac ttagtgcagc acgtcacctg
     8521 atgcgcacac agaaggacaa aaatggcgaa gtgctgaata ttgcgcctaa attcctttta
     8581 gttccggcag cactggaaga tcgcgcgttg cagatgatta actcaaccgc acctttcggg
     8641 gctgataaaa acagcgggat ctttaacccg tatcacaagc tacttgatat catcgtcgat
     8701 ccccgccttg atgatatcag cgaaaaacaa tggtacatgc tttccgcaca gggaacggac
     8761 acaatcgagg tggcttatct tgatggcagt gacgagcctt accttgaaca gcaggaaggt
     8821 tttatcgttg acggcgtggc ctggaaagtc cgtattgatg caggtgtggc agctctggat
     8881 tatcgcggta tggtcaaatc aggcgggaca gattcactat gacaacaagg cggcaccagc
     8941 cgcct
//
LOCUS       DAZFDC010000159         8855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFDC010000159 DAZFDC010000000
VERSION     DAZFDC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.159"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1648
                     /locus_tag="ACHJ48_004184"
     CDS             <1..1648
                     /locus_tag="ACHJ48_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4709625.1"
                     /translation="EHKSRNSLLFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGR
                     KHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLL
                     GDKRIEGSVWPKSIRGSTPKIKGTCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDE
                     STPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAR
                     GDEIPPPRSIMFHIWTAYSPFTTWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVG
                     EKLDHQVLMDKVVRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIII
                     MGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRV
                     LPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADPSPADEATSYAI
                     RFPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVS
                     VQRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
     gene            1632..1838
                     /locus_tag="ACHJ48_004185"
     CDS             1632..1838
                     /locus_tag="ACHJ48_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO4709626.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            1835..3427
                     /locus_tag="ACHJ48_004186"
     CDS             1835..3427
                     /locus_tag="ACHJ48_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4709627.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSYWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKEPALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3417..4922
                     /locus_tag="ACHJ48_004187"
     CDS             3417..4922
                     /locus_tag="ACHJ48_004187"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4709628.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQNMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            4959..5306
                     /locus_tag="ACHJ48_004188"
     CDS             4959..5306
                     /locus_tag="ACHJ48_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4709629.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            5364..5615
                     /locus_tag="ACHJ48_004189"
                     /pseudo
     CDS             5364..5615
                     /locus_tag="ACHJ48_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            5612..6352
                     /locus_tag="ACHJ48_004190"
     CDS             5612..6352
                     /locus_tag="ACHJ48_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4709630.1"
                     /translation="MPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKDLTPG
                     EMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVR
                     AYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVSAI
                     KVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATG
                     KVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            6366..6797
                     /locus_tag="ACHJ48_004191"
     CDS             6366..6797
                     /locus_tag="ACHJ48_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4709631.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMSGAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            6824..7237
                     /locus_tag="ACHJ48_004192"
     CDS             6824..7237
                     /locus_tag="ACHJ48_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4709632.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            7218..>8855
                     /locus_tag="ACHJ48_004193"
     CDS             7218..>8855
                     /locus_tag="ACHJ48_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4709633.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMVRQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILRADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKR"
BASE COUNT         2023 a         2180 c         2830 g         1822 t
ORIGIN      
        1 tgagcataaa tcccgaaaca gtctgctttt tcagcccacg gattctgccg ctgaagattt
       61 tatgaagtct cacgtggagg cgacgattcg gaacgtgcca tgcctgaaag acctttcccc
      121 atggctgggt cgtaaacatc gtgacaatac tctcacgctg aaacgctttt catcgggcgt
      181 cggtttctgg tgcctgggcg gcgctgccgc caaaaactac cgtgaaaaat ccgttgacgt
      241 ggtctgctat gacgaacttt cctcgttcga gccggatgtc gaaaaagagg gctcgccaac
      301 cctgctgggg gataagcgta ttgaggggtc ggtgtggcca aaatccattc gcggctcgac
      361 gcctaaaatc aaaggcacct gccagatcga aaaagccgct aacgagtcgg cgcattttat
      421 gcgtttttat gtgccctgcc cgcactgtgg ggaggcgcag tatctgaaat ttggcgatga
      481 gtccacgcct tttgggctta aatgggagaa ggacagccct gaaagtgttt tctacctctg
      541 tgaacatcat ggctgcgtga tccatcagtc tgaactggac cagagcaacg ggcggtggat
      601 ctgtgaaaac acgggcatgt ggacccgtga cggtctgacg tttttcagcg cccggggtga
      661 tgaaattccg ccgccgcgct ccatcatgtt ccatatctgg acggcgtaca gtccgttcac
      721 cacctgggta cagattgtct atgactggct ggatgcactg aaagatccca acggcctgaa
      781 aacctttgtg aacaccacgc tgggcgagac ctgggaagag gccgtgggcg aaaaactcga
      841 tcaccaggta ctgatggata aggtggtgcg ttacacggcg gcggtgcctg cccgggtggt
      901 ttatctgacg gcgggcattg actcgcagcg aaaccgtttt gagatgtatg tctggggatg
      961 ggctccggga gaggaagcct ttctggtgga taaaatcatc attatggggc gtcctgatga
     1021 ggaagagacg ctgttacgtg tggatgcggc gatcaacaaa aaataccgcc atgcggatgg
     1081 caccgaaatg actatttccc gtgtctgctg ggacaccggg gggatcgatg gtgaaattgt
     1141 ttatcagaga tcaaaaaaac acggtgtttt ccgggtgctg ccggtaaaag gcgcatctgt
     1201 ctatggcaag ccggtgatca ccatgccaaa aacccgcaat cagcggggcg tgtatctgtg
     1261 tgaagtggga acggacaccg caaaagaaat tctctatgcc cgtatgaaag ccgatccctc
     1321 gcctgcggat gaagccacgt cgtatgccat ccgttttcct gatgatccgg agattttttc
     1381 gcagacagag gcgcagcaac tggtggcgga agagctggtg gagaagtggg aaaaaggaaa
     1441 gatgcgtctg ctgtgggata acaaaaagcg gcgtaacgaa gcgctggact gcctggtgta
     1501 tgcctacgcg gcattacgtg tgtccgtgca acgctggcag cttgatctgg ctgtactggc
     1561 aaaatcccgg gaagaagaga cgacccggcc aaccctgaaa gaactggcag cgaagctgtc
     1621 cggaggagtg aatggttaca gtcgctgaac tgcaggcgct gcgtcaggcg cgccttgatt
     1681 tattaaccgg taaacgggtg gtgtctgtcc agaaagatgg tcgcagaatt gaatatacgg
     1741 cggcttctct ggatgagctt aaccgggcga tcaatgatgc ggagtcggta ctggggacaa
     1801 cccggcgtcg ccgtcgtccg ctgggagtga ggttatgaaa cgaacgcctg tcctgattga
     1861 tgtgaacggc gttccgcttc gtgagagtct cagctacaac gggggcggtg caggatttgg
     1921 cgggcaaatg gcggagtggt tgccaccggc gcagagtgcc gatgcggccc tgctgcccgc
     1981 gttgcgtctg gggaatgccc gggcagatga tctggtgcgc aataacggaa tagcggccaa
     2041 tgcggtggca ctgcataagg atcacattgt cgggcatatg tttcttatca gctaccgtcc
     2101 gaactggcgc tggctgggga tgcgggagac cgcagcaaaa agctttgtcg atgaggtgga
     2161 ggcggcctgg tcggaatacg ccgaagggat gtctggcgag atcgacgtgg aaggaaaacg
     2221 cacgttcacg gaatttatcc gtgaaggtgt gggcgttcat gcgtttaacg gcgaaatctt
     2281 tgtgcagccg gtctgggata cggaaaccac gcagttattc cgtacgcgtt ttaaagccgt
     2341 gagtccgaaa cgggtggaca cgccaggaca cggtatgggg aaccgttttc tgcgggccgg
     2401 ggtggaggtc gatcgatatg gccgtgccgt tgcgtaccat atctgtgagg atgattttcc
     2461 tcgctccggg agtggacgat gggaacggat cccgcgtgaa cttcccaccg ggcgtccggc
     2521 catgctgcat attttcgagc cggtggagga cgggcagacc cgtggggcca accagtttta
     2581 cagcgtcatg gaacggctga agatgctcga ttccctgcag gcaacacagc ttcagtcggc
     2641 cattgtgaaa gccatgtatg cagcgacgat tgaaagtgac cttgataccg aaaaggcctt
     2701 tgaatatatc gccggtgcgc cgcaggggca gaaggataat ccgcttatta atattctgga
     2761 gaagttctcc agctggtatg acacgaataa cgtgacgctg ggtggtgtca aaattccgca
     2821 ccttttcccc ggggatgatc tgaaactaca gactgcgcag gattcagaca atggattttc
     2881 ggcgcttgaa caggcgctgc tgcggtatat cgccgccggt cttggcgttt cctacgaaca
     2941 gttgtcccgt gattactcga aggtcagtta ttcaagtgcc agggcctctg ccaatgagtc
     3001 gtggcgctat tttatggggc ggcgaaaatt tattgcggcc cggctggcca cgcagatgtt
     3061 ttcctactgg ctggaagagg cacttcttcg ggggattatc cgtccgccac gggcgcgttt
     3121 tgatttttat caggcgcgat cagcctggtc acgggcagag tggattggtg ccggaagaat
     3181 ggccattgac gggctcaagg aggttcagga atcggtgatg cgcattgagg ccggactgag
     3241 cacgtatgag aaagagccgg cgctgatggg cgaggattat caggacattt tccgccagca
     3301 ggtcagggaa tctgcagagc ggcaaaaagc cggactctca cgtccggtgt ggatagcgca
     3361 ggcgtatcag cagcagatag cggagagtcg caggccggaa gaggagacaa caccccgtga
     3421 gacgtaatct ttcacacatt attgccgcag cattcaatga accgctgctt ctggagcccg
     3481 cctatgcgcg ggttttcttt tgcgcgctcg ggcgcgagat gggggcagca agtctttcgg
     3541 taccacagca gcaggtacag cttgatgctc ccggaatgct ggctgaaacg gacgagtaca
     3601 tggccggagg taaacgaccg gcccgtgttt accgggtggt gaacggtatt gctgtactgc
     3661 cggtgaccgg cacgctggtg caccggctgg ggggtatgcg gccattttcc ggaatgacag
     3721 gctatgacgg cattgtcgcc tgtcttcagc aggcaatggc ggatagccag gtgcggggcg
     3781 tactgctgga cattgacagt ccgggcgggc aggccgccgg cgcgtttgac tgcgctgaca
     3841 tgatttaccg cctccgtcag cagaagccgg tctgggcact gtgcaatgac acggcctgtt
     3901 ctgcagccat gctgctggcg tcggcctgct cccgacggct ggttacccag acatcccgta
     3961 tcggctccat tggcgtgatg atgagccatg tcagctatgc cggtcatctg gcgcaggccg
     4021 gtgtggatat cacgctgatt tactcagggg cgcacaaggt ggatggcaat cagtttgaag
     4081 ccttaccggc agaggttcgc cagaacatgc agcagcgcat tgatgcggcg cgccggatgt
     4141 ttgccgaaaa agtggccatg tttaccggtc tgtctgttga tgccgtcacg ggaacagagg
     4201 ccgccgtttt tgaaggtcag tccggcattg atgccgggct ggcggatgaa ttagtcaatg
     4261 cgtcggatgc catcagtgtg atggccacgg cgctgaacag taatgtcaga ggaggcacta
     4321 tgccgcaatt aactgcaacg gaagccgccg cgcaggagaa ccagcgagtg atggggatcc
     4381 tgacatgcca ggaagcgaaa ggacgtgaac agcttgccac gatgctggca ggacaacagg
     4441 gcatgagcgt tgaacaggcc cgggcgattc tggccgcggc ggcaccgcag cagccggtgg
     4501 catccacgca gagtgaagcc gatcgcatta tggcgtgtga agaagcgaac ggtcgtgaac
     4561 aactggcggc aacgctggcg gcgatgccgg agatgacggt ggaaaaagcc cgcccgatcc
     4621 tggctgcttc accgcaggcg gatgccggac cctcactccg tgatcagatc atggcactgg
     4681 atgaggcaaa aggggctgag gcgcaggctg aacagctggc tgcctgcccg ggaatgactg
     4741 tggagagcgc ccgggctgtg ctggctgcgg gatcaggtaa ggcagaaccg gtctctgcat
     4801 ccacaaccgc cctgtttgaa cgcatcatgg cgaaccattc accggctgcg gtacagggtg
     4861 gcgtgccaca gacgtcagca gacggtgatg cggacgtgaa aatgctcatg gccatgccat
     4921 gaagtcagtg ctgaccatca acaggaggtt tttacaatat ggtaacgaaa aacatcactg
     4981 aacagcgtgc ggaagtacgt atttttgccg gtaatgatcc ggctcatacc gccacaggca
     5041 gcagcgggat ttcctcggca acaccggcac tgacgcccct gatgctggat gaagccagcg
     5101 ggaaactggt ggtctgggac ggacagaaag ccggtagtgc agttggcata ctggtactgc
     5161 cgcttgaagg cacagagacg gtactgacct attacaagtc ggggaccttt gcgacggagg
     5221 caatccgctg gcctgaaagt gtggatgaac acaaaaaggc aaatgccttt gccggcagtg
     5281 ccctgagtca cgcggcgctg ccgtaacacg ttatcaggcc accgcggtgg cctgactgat
     5341 ttctgaatga aaggaactga tttatgggat tgtttacgac ccgccagtta ctcggttata
     5401 ccgaacaaaa agtgaaattt cgtgcgctgt ttctggagct gtttttccgc cgtacggtga
     5461 atttccatac cgaagaggtg atgctggaca aaattaccgg aaaaacgccg gtggcggcct
     5521 atgtctcccc ggttgttgaa ggaaaagtgc tgcgtcatcg cggtggtgaa acccgcgtgt
     5581 tacgtccggg ctacgtcaag ccgaaacacg aattcccctg gagccggtaa aaggagccgg
     5641 taccaccctg tgggtttata acggtcaggg tgacgcctat gcaaacccgt tgtcagacga
     5701 tgactggcag cgactggcta aggtgaagga tctgacgccg ggcgagatga cggcagaatc
     5761 ctacgatgat aactacctgg atgatgaaga cgcggactgg accgcgaccg ggcaggggca
     5821 gaaatctgca ggtgatacca gttttacgct ggcctggaaa ccgggagagg aaggccagaa
     5881 agggcttata ggctggtttg aaagcggcga tgtccgggcc tataaaatcc gttttccgaa
     5941 tggcacggtg gatgtgtttc gtggctgggt cagcagtatc ggtaaggccg tgacggcgaa
     6001 agaagtgatc acccgcacgg tgaaagtcac taacgtgggt aaaccttctg tagcggaaga
     6061 acgcagcaaa attacgccgg tcagtgcgat taaggtgacg ccgacatccg gtacggtggc
     6121 aaaagggaaa acaaccaccc tgacggtttc ttttgagccg gaaagtgcaa cagacaagac
     6181 gttcagagcg gtttccgccg atccgtcgaa agccaccatt agtgtgaaag atatgacaat
     6241 tacggtaaac ggcgtggcga caggtaaggt gcagatccct gtggtgagcg gaaatggtca
     6301 gttcgccgca gtggctgaag tcaccgttac tgaagcgggc gctgcagggt aaacggaggt
     6361 catacatgtt tctgaaaaca gaacaatttg aatataacgg tgtgtctgtc acgctttccg
     6421 aattgtctgc gctgcagcgt atcgagcatc ttgccctcct gaaacggcgt gcagaacagg
     6481 cagaatccag cggcaacctg caggtaagcg tggaagatct cgtcagaacc ggcgcgtttc
     6541 tggtggcgat gtccctgtgg cataaccatc cacagaaaac gcagtcaccg tcaatgaatg
     6601 aggccgtgat gaagatagag caggaagtgc tcaccacctg gcctgccgat gccattgccc
     6661 gggcggaaga cgtggtgttg tgcctgtccg ggatgagcgg ggctgttcgt ccggatactg
     6721 atattactga agtggcgaaa aataacacgc tgactgatga tgatttttct gcgggaaagt
     6781 cttcgacggc gagctgaact ttgccctcag actggcgcgt gagatgggga gacccgactg
     6841 gcgcgccatg cttgccggga tgacatccac cgaatatgcc gactggcacc gtttttaccg
     6901 cacgcattat tttcaggata cccagctgga tatgcatttt tccgggctga cgtacgctgt
     6961 actcagcctg tttttttgcg atccggatat gcatccctct gatttcagtc tgcttgtccc
     7021 ccggcatgag gaagagcagg tggagaggcc ggatgaggac aaaatgctga tgcagaaagc
     7081 ggcaggactt gccggaggcg tccggttcgg tggggacgga gggcgcgata ttttatcgtc
     7141 tgcggatgtg gcggatgtca tggtggatga tgccgcatta atgatggctt cagcggggat
     7201 tccgggaggt gtgagatatg tcccagccgg ttggtgatct tgttattgac ctgagtctgg
     7261 atgctgtccg tttcgatgag cagatgagcc gggtaaggcg tcatttttca ggtctggata
     7321 ccgacgtcag aaaaaccgcc agtgctgttg aacagggcct gagccgccag gcgctggctg
     7381 cacaaaaagc cgggatttcc gtcgggcagt ataaagcggc catgcgaacc ctgcccgcac
     7441 agtttacgga tatcgccacg cagcttgccg gtggtcagaa tccctggctg atcctgctgc
     7501 aacagggcgg tcaggtgaag gactccttcg gcgggatgat ccccatgttc agggggcttg
     7561 ccggtgcgat caccctgccg atggtcgggg tcacctcgct ggcggtggcg acaggtgcgc
     7621 tggtgtacgc ctggtaccag ggagattcca cgctttcagc gtttaataaa accctggttc
     7681 tttccggtaa tcagtccgga ctgactgccg atcgcatgct gacgctctca agagccgggc
     7741 aggcagcagg gctgacgttt aaccaggcga gagagtcact ggcagccctg gtgaatgccg
     7801 gtgtgcgtgg tggtgaacag tttgatgcca tcaaccagag tgtcgcgcgt tttgcttctg
     7861 catccggtgt ggaggtggac aaggttgcag aggctttcgg aaaactgacc accgacccta
     7921 cgtcggggct gattgcgatg gtgcgccagt tccgtaacgt gacggcagag cagattgcgt
     7981 atgttgcgca gctgcagcgt tccggtgatg aggccggggc cttacaggcg gcgaacgata
     8041 tcgccacgaa aggctttgat gagcagaccc gtcgcctgaa agaaaacatg gggacactgg
     8101 agacctgggc ggataaaacc gggaaggcat tcaaatcgat gtgggatgcc attctggata
     8161 tcggtcgtcc tgagtcctca gcggatatgc tcgccagtgc acagaaggca tttgatgagg
     8221 cggataaaaa atggcagtgg taccagagcc ggagccagcg ccggggaaaa accgcctctt
     8281 tccgggccaa ccttcagggc gcatggaatg accgggaaaa tgcccgtctg gggctggcag
     8341 cggccacgct gcagtcggat atggaaaaag ccggtgaact ggccgccagg gaccgggccg
     8401 aacgggacgc atcacagctg aagtataccg gagaggcgca gaaggcgtat gagcgtctgc
     8461 tgacgccgct ggagaaatat accgcccgtc aggaagaact gaataaggcc ctgaaagacg
     8521 ggaaaatcct gcgggcggat tacaacacgc tgatggcggc ggcgaaaaag gattatgaat
     8581 cgacgctgaa aaagccgaag tcgtcaggag tcaaagtgtc agccggtgag cgtcaggaag
     8641 accaggcgca tgctgccctg ctggcgcttg aaaccgagct caggacgctg gaaaaacaca
     8701 gcggtgcgaa tgagaaaatc agccagcagc gtcgcgattt atggaaagcg gaaaatcagt
     8761 atgcggtcct gaaagaggct gccacgaaac ggcagttatc tgagcaggaa aaatccctgc
     8821 tggcgcataa agacgagacg ctggagtaca aacgc
//
LOCUS       DAZFDC010000160         8740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFDC010000160 DAZFDC010000000
VERSION     DAZFDC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.160"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..690
                     /locus_tag="ACHJ48_004194"
     CDS             <1..690
                     /locus_tag="ACHJ48_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO4709634.1"
                     /translation="AVKLVMQLKILNIVNKNFIENMKKIFSHGPYIKDPIIRSYIHPD
                     EDNKFDNFMRQNRFSKVNFDTQQMIDFINRFNMNKWLIDRNNNFFIQLIDQALRSTND
                     TIKENAWHLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRNDNIFILFSSVDDGPVMV
                     VSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTPETLFGSKSYDKHALFPIFTAS
                     WRANRIKNKGI"
     gene            913..1410
                     /gene="ubiC"
                     /locus_tag="ACHJ48_004195"
     CDS             913..1410
                     /gene="ubiC"
                     /locus_tag="ACHJ48_004195"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO4709635.1"
                     /translation="MSHPALTQLRALRYCKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1423..2295
                     /gene="ubiA"
                     /locus_tag="ACHJ48_004196"
     CDS             1423..2295
                     /gene="ubiA"
                     /locus_tag="ACHJ48_004196"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO4709636.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2450..4873)
                     /gene="plsB"
                     /locus_tag="ACHJ48_004197"
     CDS             complement(2450..4873)
                     /gene="plsB"
                     /locus_tag="ACHJ48_004197"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO4709637.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5044..5412
                     /gene="dgkA"
                     /locus_tag="ACHJ48_004198"
     CDS             5044..5412
                     /gene="dgkA"
                     /locus_tag="ACHJ48_004198"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO4709638.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5522..6130
                     /gene="lexA"
                     /locus_tag="ACHJ48_004199"
     CDS             5522..6130
                     /gene="lexA"
                     /locus_tag="ACHJ48_004199"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO4709639.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6203..7528
                     /gene="dinF"
                     /locus_tag="ACHJ48_004200"
     CDS             6203..7528
                     /gene="dinF"
                     /locus_tag="ACHJ48_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO4709640.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQSYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVRGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTSWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLASLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7644..7853
                     /gene="yjbJ"
                     /locus_tag="ACHJ48_004201"
     CDS             7644..7853
                     /gene="yjbJ"
                     /locus_tag="ACHJ48_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO4709641.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7895..8410)
                     /gene="zur"
                     /locus_tag="ACHJ48_004202"
     CDS             complement(7895..8410)
                     /gene="zur"
                     /locus_tag="ACHJ48_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO4709642.1"
                     /translation="MEKTTTQELLAQADKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2059 a         2080 c         2330 g         2271 t
ORIGIN      
        1 gcagtaaaac tggtgatgca attaaaaatt cttaatattg ttaataaaaa ctttattgag
       61 aatatgaaga aaatatttag ccatggtcct tatataaaag atcccattat acgtagttat
      121 atccatcctg atgaagataa caagttcgat aattttatgc gtcaaaatcg attcagtaag
      181 gtgaatttcg atacccaaca gatgatcgat tttattaaca gatttaatat gaataaatgg
      241 ctgattgatc gaaataacaa tttttttatc caacttatcg atcaggctct acgatcaacg
      301 aatgatacga tcaaagaaaa tgcctggcat ctttataaag agtggattcg tagtgatgat
      361 gtttcacctt tatttataga aattgaagat aatttaagaa cctttaacac gaatgaatta
      421 acacgaaacg ataatatctt tatcttgttc tcctctgtcg atgatgggcc agttatggtg
      481 gtaagctccc agcgcttaca tgatatgttg aatcctacaa aagataccaa ttggaattcc
      541 acgtatatct ataaatccag acatgagatg ttgcctgtta atcttactcc ggaaacactt
      601 ttcggctcca aatcttatga taaacatgcg cttttcccca tttttactgc gagttggcga
      661 gctaatcgta taaagaataa aggtatttaa gtaaaggaaa acatcaccgc tcttggcatc
      721 atgggcggtg atgcccctat ggttgccctc gccagcacgg gcatcggcaa agcgtaaggt
      781 tcaacagcgt tttaccactt catgcgattg ttgcgttttt gttgcgcatt agatcactta
      841 atttgcttta catctcccgt aaacactttt ctgcgataca atgcctttac gttatgtaac
      901 ggagagttcg gcatgtcaca ccccgcgtta acgcaactgc gtgcgctgcg ctattgtaaa
      961 gagatccctg ccctggagcc gcaactgctc gactggctgt tgctggaaga ttccatgaca
     1021 aaacgttttg aacagcaggg aaaaacggtg agcgtgacga tgatccgcga agggtttgtc
     1081 gagcagaatg aaatccccga agaactgccg ctgctgccga aagagtctcg ttactggtta
     1141 cgtgaaattt tgttatgtgc cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct
     1201 gtgtcaacgt taagcgggcc ggagctggcg ttacaaaaat tgggtaaaac gccgttagga
     1261 cgctatctgt tcacatcatc gacattaacc cgggacttta ttgagatagg ccgtgatgcc
     1321 ggactgtggg ggcgacgttc ccgcttgcga ttaagcggta aaccgctgtt gctaacagaa
     1381 ctgtttttac cggcgtcacc gttgtactaa gaggaaaaaa atatggagtg gagtctgacg
     1441 cagaataagc tgctggcgtt tcatcgctta atgcgtacgg ataagccaat tggcgcgtta
     1501 ctgctgctct ggccaacatt atgggcgttg tgggtggcga caccgggcgt tccccagctc
     1561 tggatcctgg cggtgtttgt cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg
     1621 aatgattatg ctgaccgcaa gtttgatggt catgttaagc gcacggcgaa ccgaccactt
     1681 cccagcggcg cggtaacaga gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt
     1741 tcgtttttac tggtgctgac gctgaatacg atgaccattc tgttgtcgat tgccgcgcta
     1801 gcgctggcgt gggtgtaccc gtttatgaag cggtataccc atctaccgca agtggtgctg
     1861 ggcgcggcgt ttggctggtc gattccaatg gcttttgctg ctgtgagtga gtcggtgcca
     1921 ttgagttgct ggttaatgtt cctcgccaat attctctggg cggtggctta cgacacgcag
     1981 tatgcgatgg ttgaccgtga tgatgatgtg aagattggca ttaaatccac cgcaatcctg
     2041 ttcggccaat acgataaatt gattattggt attttgcaga ttggcgtact ggcgctgatg
     2101 gcgattatcg gtgagttaaa tggcttaggc tggggatatt actggtcaat tctggtggct
     2161 ggcgcgctgt ttgtttatca acaaaaactg attgccaacc gcgagcgcga agcctgcttt
     2221 aaagcattta tgaataataa ctatgttggt ctggtactgt ttttagggct ggcaatgagt
     2281 tactggcatt tctgatgatg taaaaaagcc ggatgatcat ccggctttct tctgggttgc
     2341 ctgatgcgct gcgcttctca ggcctacaca acagatcgca atttattgaa tttgcagatt
     2401 atggaaggcc ggataaggcg tttgcgccgc atccggcaat tctctctgat tacccttcgc
     2461 cctgcgtcgc actctcaatc gtcaaacgca cgtctgatgt aatcaactcc gccagcaact
     2521 gataaacctt catcgtttct gccggttcgg catcgccgct atcgctgata tacccttcat
     2581 cacgcagcgt aagcaccaga gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga
     2641 tgccgtgcag cacggagaga cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc
     2701 ggttgatcga cgggttggcg ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt
     2761 cgcgcgcgcc tgcggcgagc agctgtagcg tgcgagaatg caccgggttg atatgcaact
     2821 catcatcttg cagggtaatc agcccctgac gttgcatctc atttgccagc gcatcaataa
     2881 cgtccggcaa ctcgtcgcga tcccagcgca ggaacagctc cgctttcagc attgggtaaa
     2941 gcacattgac gtgctccatc aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg
     3001 ccgccatcag cgaaggtagc accaacatat gcgcaatgtt gttgcgatag taggtcatca
     3061 gcaccgcttg ctcgcgcggc agaatgatga tgtcgccgat agtgtctttc tcaacttcaa
     3121 acttgttcat ttgcagcgcg tgatcgataa gctcgctggc gctggctgaa ggaacggtag
     3181 agtccgtgga gtaaggcacg ttgcgcatca gatccaggta gcagttgagt tgctcggtta
     3241 actgctcgcg ggtgagtgag cgctgacgtg atgccagtag cgcagtacag cacaggttca
     3301 tggcgtttgc cgcgcctgcg ttgttaatgc gtaccatcag atcggcagca atattattga
     3361 ccgtcggcgt taaccatgcc ggacgcactg cttcgatggg atcgatagat tcacgccagt
     3421 ctggtacgtg ctggttaagg taggtcatca acggcattgg ttcaccgaaa ttgacgtaac
     3481 cctggccgag attacgcagc ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct
     3541 ctttcgtcgc gccgcgcagt tctttggcgt aagtacccac ttccatgacg tgctcataac
     3601 cgatatagat cggaatcagc gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg
     3661 tcatcgacag cgtaccggtt ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt
     3721 ctacgaagta ctcgacggaa taaccacggc tgaacagttc gccgagatac tcacggaaaa
     3781 cggtggaata gagcttattg cctttaaaag tacggcgaat aaagaacgca cccagacggc
     3841 ggaaaatcgg cccggcaggc cagaaattca ggttgatccc ggcggcgata tgcggcggca
     3901 ctagcccctg gtggtacagc acgtaagaaa gcagcaggta gtccatgtga ctgcggtggc
     3961 aaggcacata taccagctca tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat
     4021 ggacgttgat gccctggtaa aggcggttcc aggtaaagcc cagaatacgg tcagtcaggc
     4081 ggatcatctc gtaagagaaa ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct
     4141 gcgctttttc atgggagatt tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg
     4201 cgcgggaggc gagcagctta ttaaacagat cctgacgagc aggcagacgt ggtcctacgg
     4261 cagccagacg ttgacgggca aagtgcatac gcgccacgcg cgccagtttc tgcgcgatag
     4321 ttttatccgt gccgtgttca tccgccatac ggcgcagcga aactgacggc gagaaacgca
     4381 caaaactgtc gcgaccgagc cacagtacag cgaaaaattt ctgtacgccg ttaagcatac
     4441 gcagcggcgg gttcacttcg cctttttcac gccccggcgc gcgaccaaac atcaccgaca
     4501 ctggcaccat ctgcacatcc agatttgggt tgctacggtg caaatcgaga tagtcgtgga
     4561 acagcttaat agactcttct ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa
     4621 tgaacacata gcgcggcagt agcgtgccgt cgatttccag cggctctaac gggtcaggca
     4681 agtcatgtgc cagacactgg gcgcgcaacg tcagcaaatc tgctttcgag ttgtacggta
     4741 aaacgtacat aattggacga gaggtatcca gccccagttc cggggcagga tctgccggaa
     4801 tagacttgct ttttaccagg atgcttaatg gtaaattcag taatttgtag taaattcgtg
     4861 gccagccgga cataaacgat gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat
     4921 agcagaaagt catgggaaat tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa
     4981 cccgaatcat cggatttaac ggtacactga tattgacgct cataatgtaa aaaggttctt
     5041 tcaatggcca ataataccac tggattcacc cgaataatca aagctgctgg ctattcctgg
     5101 aaaggtttac gcgctgcatg gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta
     5161 ttgttggcgg tggtcatcgc ctgctggctg gatgtggacg cgattacccg cgtgctgctt
     5221 atcagctccg tgatgctggt gatgattgtg gaaatcctca atagcgccat cgaagcagtg
     5281 gttgaccgaa ttggctctga ataccatgag ctttccggac gcgctaaaga tatgggatcc
     5341 gccgcggtgc tgattgcgat tatcgtcgcc gtgattacct ggtgcatcct gttatggtcg
     5401 cattttggat aacccttcca gaattcgata aatctctggt ttattgtgca gtttatggtt
     5461 ccaaaatcgc cttttgctgt atatactcac agcataactg tatatacacc cagggggcgg
     5521 aatgaaagcg ttaacggcca ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag
     5581 ccagacaggt atgccgccga cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc
     5641 aaacgcggct gaagaacatc tgaaggcgct ggcacgcaaa ggcgtaattg aaattgtttc
     5701 cggcgcatca cgcgggattc gtctgttgca ggaagaggaa gaagggttgc cactggtagg
     5761 tcgtgtggct gccggtgaac cgcttctggc gcaacagcat attgaaggtc attatcaggt
     5821 cgatccttcc ttgttcaagc cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat
     5881 gaaagatatc ggcattatgg atggcgactt gctggcagtg cataaaactc aggatgtacg
     5941 taacggtcag gtcgttgtcg cacgtattga tgacgaagtt accgttaagc gcctgaaaaa
     6001 acagggcaat aaagtcgaac tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga
     6061 ccttcgtcag cagagcttca ccattgaagg gctggcggtt ggcgttattc gcaacggcga
     6121 ctggctgtaa catatctctg agaccgcgat gccgcttggc gtcgcggttt gtttttcatc
     6181 tctcttcatc aggcttgtct gcatggcatt cctcacttca tctgataaag cactttggca
     6241 tctcgcctta cccatgattt tctccaatat cactgttccg ttgctgggac tggtcgatac
     6301 ggcggttatt ggtcatcttg atagtccggt ttatttgggc ggcgtggcgg ttggtgcaac
     6361 ggcgaccagc tttctcttta tgctgttgct gtttttacgc atgagcacca ccgggctgac
     6421 tgcgcagtct tatggtgcca aaaatcctca ggcattagcc cgtgcgctgg tgcaaccttt
     6481 gctgttggcg ttgggggctg gggcgttaat tgcgctgctg cgtacgccga ttatcgatct
     6541 ggcgctgcat attgttggcg gtagtgaagc agtcctggaa caggcgcggc gctttcttga
     6601 aatccgctgg ttaagcgcac cggcgtcgct ggcgaatctg gtattacttg gttggttact
     6661 cggcgtgcaa tatgcccgtg cgccagtaat tttgttagtg gtcggcaata tcctcaacat
     6721 tgtgctggat gtctggctgg tgatgggact gcatatgaac gtgcggggcg cggcgctggc
     6781 gacggttatt gcggaatatg caacattgct gattggtctg ctaatggtgc gtaaaatcct
     6841 caaactacgc ggaatctccg gcgaaatgct gaaaacttcc tggcgaggaa acttccgtcg
     6901 cttgctggca cttaaccgcg atatcatgct gcgctcgctg ttgttgcaac tctgtttcgg
     6961 cgcgatcacc gtacttggcg cgcgactggg gagtgacatt atcgctgtta acgcggttct
     7021 gatgacgcta ctcaccttta ccgcctatgc gctggacggt tttgcctacg cggttgaagc
     7081 acattccggt caggcatacg gtgcgcgcga cggtagccag ttgctggatg tctggcgggc
     7141 agcatgccgc cagtcgggga tcgtagcgtt actgttttcg gtggtttatt tgctggcagg
     7201 ggaacacatc attgcgttac tggcgtcgtt aacccagatt cagcagctgg ccgaccgcta
     7261 tcttatctgg caggtgattt tgccgttggt cggcgtctgg tgttatctgc tggacggcat
     7321 gtttataggc gcaacgcgtg ctgctgaaat gcgtaacagt atggcggtgg ccgccgcagg
     7381 ttttgcgctg acgctcctta cgctgccgtg gctgggtaat catggtttgt ggttggcgtt
     7441 aaccgtcttt ctggcgttgc gcgggctttc tctggcagct atctggcggc gtcactggcg
     7501 caacggtacc tggtttgccg caacgtgacg gttaaaaatt ctgaataaat aatcctaagc
     7561 caaattgctg actacactta atctcacgtt cagaagaaaa gtgaacgtac tctcattcac
     7621 aacctaacga tgaggtcttg attatgaata aagatgaagc cggcggtaac tggaaacagt
     7681 ttaaaggtaa agtgaaagag caatggggca aactgaccga tgatgatatg acgatcattg
     7741 aaggtaaacg tgatcaactg gtcggtaaaa tccaggaacg ttatggttat cagaaagatc
     7801 aggcagaaaa agaggtcgtg gattgggaaa cccgcaatga atatcgctgg taattaatcc
     7861 ctcctgcccg acgtgtacaa ggatgtacgc cctcttaacg cggtttcttt ttcacctgca
     7921 cagagtgatc atgctggcac tgttcaggat gacgacacgc ttccacttcc acgcatgccg
     7981 cacagagccc atgtgcttca atcacattat gccgcagggc aaaccccatt tttgccgcca
     8041 gcgtatgcat gatgtcttcc acgccttcag cacattcttc tttcaccgcg ccgcagcgat
     8101 cgcaaataaa catagctgac gtatgggtgg gctggtcgaa cagatggcag agcacataac
     8161 tgttggtgga ttccacctta tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat
     8221 aaaccgttgg cggcttggct tgcggttcag cttcgcgcag caaatcaagc agatcataag
     8281 cgctgatagc gccatcctgc agactcatca ggcgcaacac ttccaggcgc tgtggggtca
     8341 ggcgcacatt acgctgcgcg cagattttgt cagcctgcgc taataactcc tgcgttgtgg
     8401 tcttttccat cgggcacctc aaagtggggg ttaagaatgt ccttacttta ccatgttcca
     8461 ggaaaaacac cgagaaccct tattgttgcc gtaatgttgc ttttctgttt tgtaggtaag
     8521 atgttgtgtt gcctggcagt accattatca acacgataat aattaataat tcatttttaa
     8581 ataaattgct tcaagttaac tcgctattta tttaaccgtg gctaatcgta tacccgaatt
     8641 tcagaaaatt atgagcgatt tttgatagtt accggttcca aaaacactta taaggaggac
     8701 acggtacgac aaaaaaggta gatgaatcat gttaaagata
//
LOCUS       DAZFDC010000161         8729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFDC010000161 DAZFDC010000000
VERSION     DAZFDC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.161"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..300
                     /locus_tag="ACHJ48_004203"
     CDS             <1..300
                     /locus_tag="ACHJ48_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4709643.1"
                     /translation="EELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNS
                     MNSNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDLDRGNFVLKN"
     gene            331..450
                     /locus_tag="ACHJ48_004204"
                     /pseudo
     CDS             331..450
                     /locus_tag="ACHJ48_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(461..709)
                     /locus_tag="ACHJ48_004205"
     CDS             complement(461..709)
                     /locus_tag="ACHJ48_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4709644.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESSDEWFVH"
     gene            complement(771..1868)
                     /locus_tag="ACHJ48_004206"
     CDS             complement(771..1868)
                     /locus_tag="ACHJ48_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4709645.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            1957..2994
                     /gene="dusA"
                     /locus_tag="ACHJ48_004207"
     CDS             1957..2994
                     /gene="dusA"
                     /locus_tag="ACHJ48_004207"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO4709646.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            3128..3370
                     /gene="pspG"
                     /locus_tag="ACHJ48_004208"
     CDS             3128..3370
                     /gene="pspG"
                     /locus_tag="ACHJ48_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO4709647.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(3536..4519)
                     /gene="qorA"
                     /locus_tag="ACHJ48_004209"
     CDS             complement(3536..4519)
                     /gene="qorA"
                     /locus_tag="ACHJ48_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO4709648.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            4602..6017
                     /gene="dnaB"
                     /locus_tag="ACHJ48_004210"
     CDS             4602..6017
                     /gene="dnaB"
                     /locus_tag="ACHJ48_004210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO4709649.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            6070..7149
                     /gene="alr"
                     /locus_tag="ACHJ48_004211"
     CDS             6070..7149
                     /gene="alr"
                     /locus_tag="ACHJ48_004211"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO4709650.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            7402..8595
                     /gene="tyrB"
                     /locus_tag="ACHJ48_004212"
     CDS             7402..8595
                     /gene="tyrB"
                     /locus_tag="ACHJ48_004212"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO4709651.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLAMLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         2098 a         2075 c         2232 g         2324 t
ORIGIN      
        1 gaggagttgt tagataaaat tgaagtatgt gcatttaatg tggagcatgc acaattgcaa
       61 gtgccagaga gcttacgaac atgccctgtt acattatgtg aaccagaaga tggggtattt
      121 atgaggaatt caatgaattc aaatgtttgt atgttgtatg ataaaatggc attaatacat
      181 cttgttaaaa caagggcggc tcatcctttg agcagggagt caatcgcagt ttcaatgatt
      241 gtaggaagag ataattgtgc ttttgacctt gacagaggta acttcgtttt aaaaaattaa
      301 gatagcaatg gcgggttaac ttttcgtaaa atgaaattaa ttctcggtac gacatgtaaa
      361 acgcggcacc tcgtatgcaa gaacgtactg cggttggctg gtgaactttc gatagtgcta
      421 gtattgaatg atttccagcc gttaccgatt tacgtgttta ttagtgaaca aaccactcgt
      481 cagaagattc ccaggtatct ttcagagtct cctgaacaaa tgtttttgca gaatccttat
      541 ctgcagtgcg tgtaacagaa agcccatcat tgctggtggc ttttacgatc acttctacat
      601 cgtcataacg cttactgatg cgccgagtta attcttcctt taacgcatcc acagcaccgt
      661 ttggcatttt agtcattttc tctttggcta tgcagatttc aatacgcata aaaatccctc
      721 tatactgtgt ttgtatacag tgttattttt atctgtataa ataaacagtg tcaagaggtc
      781 ttgtttctgc tcttttggag ttcttcaaaa cgattatgta aagatttcgg atacagttcg
      841 gtatatacct gccatagcac gtttaatgaa cgatgccctg taacttgagc gacttcctca
      901 atactaaaac cagcctcaaa taagcgactt gccccttctc tacgcaaatc atgatatcgc
      961 agatctttaa tacctaattt gcttcttacc ctctgaaatc ctgcggtaac agaagtgctg
     1021 ttatatggaa aaatgaattc tgattttttt ggttgtcgct ggacgatatc ccaggcttcc
     1081 ccaagcaagg caactttcat atggttacct tcctttttac gtggatcttt cctatctctt
     1141 actagtatag atttttgttc ttggtcgaga tcttcccatc gtaaccggca tacttctccg
     1201 attcgcatac aggaccacac agaaaatttg aggatatcaa cgaacggaat ttttgagcat
     1261 ttatgagtag atcgttgttg aaggccttca atgagcatgt caagttcatc agatgctggt
     1321 ctacgattac gacggtttga tttaccaatc aaaccgagtt taagtagata tggacgagcg
     1381 cttttcgccg ggtttgatgt gtaattgatt ccgtatacag gttttgccgc atccagaaca
     1441 ctgccaagat aactaacatc gtggctgact gttgctgggc ctgcaccagc gttgtttctt
     1501 agcctgcaat gttcaattac gtcattttct gtcagttcag atagtttgat cgcggatatg
     1561 tcactatcca taagcagttc cagcacatat cttttagtac ggcctgcttt acctccggca
     1621 tttgggtcat ttaaatattt gtgtagtaag tcacggactg taagtccgtc aactgcattt
     1681 gatgatggaa tgccatatag atctaattcc atcactttct gtgtgcccca tgttttggca
     1741 tgagcatgtt tagggaatgt tttgctttcc ctgtaagtga taacaccttt ttctttgata
     1801 atcacattac agcgatagcg tggtgtgcca tcggatttta gtcgtttctc tatgttatag
     1861 tacgccatta cacgacctcg ttatttcggg ttcccataaa acgtgggaac ctgtgcggga
     1921 acctaacgcg agaaaaatag cctgaaatgt tcaaaaatgc acgataatca tgaagcacaa
     1981 aaaattaatc aaaccagcgt gatgcctgaa aaaactggtg tttactggaa ttctcggttt
     2041 agcgttgcac caatgctcga ctggacggac agacattgcc gctatttctt gcgtctgctt
     2101 tcccgcaata cgttgctgta taccgaaatg gtgactacag gggcgattat tcacggtaaa
     2161 ggtgattacc tggcgtacag tgaagaagaa catccggtag cgttgcaact cggcggtagc
     2221 gatccggcgg cgctggcgca gtgtgcgaag ctggcagaag cgcgtggata tgatgagatc
     2281 aacctgaatg tcggctgccc gtctgaccgg gtgcagaacg gcatgtttgg tgcgtgtctg
     2341 atgggtaatg cgcagctggt tgccgactgc gtgaaagcga tgcgcgatgt ggtgtcgatt
     2401 ccggtgacgg tgaaaacgcg tattggcatc gatgaccagg acagctatga atttctctgc
     2461 gatttcatca acaccgtttc cggcaaaggc gagtgtgaga tgttcatcat ccacgcacgt
     2521 aaagcctggc tttcggggtt aagcccgaaa gaaaaccgtg aaatcccgcc gctcgattat
     2581 ccgcgtgtgt atcaactgaa gcgtgacttt ccgcatctga cgatgtcgat taacggtggt
     2641 atcaagtcgc tggaagaggc taaagcgcat ttgcaacata tggatggcgt gatggtcggg
     2701 cgcgaagcgt atcagaatcc gggtattctg gcggcggtag accgagagat ctttggttcc
     2761 tcggataccg atgccgatcc ggtggcggta gtgcgcgcca tgtatccgta cattgagcgt
     2821 gaactcagcc aggggacgta tcttggccat attacccggc atatgttggg cttgttccag
     2881 ggtattcctg gcgcgcggca gtggcggcgt tatttaagtg aaaatgccca taaagcgggt
     2941 gcagacatta acgtgctgga acacgcgctc aaactggtgg cggataagcg ttaacttttc
     3001 accaaaaagt agtcaaattc accacgccct gcgcaccgtc gcggggcgtt ttgctgttaa
     3061 atcaatagat tatttttggc atgattcttg taatgcctgc aagagatttc atatttggga
     3121 gagcatcatg ctggaactac tttttgtgat tggctttttt gtcatgttga tggtcaccgg
     3181 cgtttcgttg ctgggcatta tcgccgcgct ggttgtggcg acggccatta tgttcctcgg
     3241 cggtatgctg gcattgatga ttaagttgct gccgtggtta ctcctggcga ttgcggtggt
     3301 gtgggttatt aaggcgatta aagcaccaaa agtgccgaaa tatcagcgtt atgaccgctg
     3361 gcgttactaa gggattgtgc ggatgatcac aacctaaggt tttatcctta gaacaaaata
     3421 ggaattgata atcaaatctg tcactattgc gcctctaaca gattcatcgt gctgtaccct
     3481 acatacagcc gaactataaa aagaaagggc ttcccaggtg gaagccctat ttcttttatg
     3541 gaatcaacag gctggaacct tgcgtcgcgc ggctttccag aatctcatgc gcacgctgcg
     3601 catccttcag cggatatttc tgctgctcgg cgacatcgac cttaatcaca ccgctggcaa
     3661 tcaaagagaa cagttcatta ctggcctcgg ttaattcctc ccgcgtggtg atatagcctt
     3721 gcagggaagg gcgtgtcaca tacaacgagc ctttctgatt gagaatgcct aagttcacac
     3781 cggtaaccgc acctgatgag ttgccaaaac tcaccattaa gccgcggcgt tgcaggcaat
     3841 ccagcgaccg ttcccaggtg tctctgccca cggaatcgta cactacgcgc acttttttac
     3901 caccggtgat ctcttttaac cgctcgacca gattctcttc acgatagtta ataacctgcc
     3961 acgcgcccgc ttttagcgcg ctctgcgctt tttgcgcggt tcctacggtg ccgataagtt
     4021 tcgcgcccag ggcttttgcc cactggcagg caatcaagcc aacgccgcca gctgctgcgt
     4081 ggaacaggaa ctgttcatcg ggtttaattt cataggtttt gcgcagcaga taataaaccg
     4141 ttaagccttt caggaaagat gctgcagctt gctcaaaaga aattgccgca ggcagaatcg
     4201 ccgctttatc cgcattaatg ttatgcacag agctgtaagc gcctaacgcc gactgcgcat
     4261 agactacacg atcgcctgcc ttaatatgct ttacaccact gccgacttta ctcacgatgc
     4321 ctgccgcttc ggtacctaat ccgctgggta gtgatggcgg cgggtaaagg ccgctgcgga
     4381 tatacgtgtc gataaaattg atgccgatgg ctttattttc gacctggatt tcattctccg
     4441 ccggatcggc aggagtgaac tctacggctt gaagtacttc cgggccaccg tgcttgtgaa
     4501 attcaattcg tgttgccatg tgtcctccag aacgtatcgt cagggtctgc ttcatatggt
     4561 aaagtttcga cccattcttt atctcggtaa ctccattcac tatggcagga aataaaccct
     4621 tcaacaaaca gcaggctgaa ccccgcgaac gcgatccaca agttgccggg ctgaaagtgc
     4681 ctccgcactc gatcgaagcg gagcagtcgg tgttgggcgg tttaatgctg gataacgaac
     4741 gctgggatga tgtagccgag cgtgtggtag cagacgattt ttacacccgc ccacaccgtc
     4801 atatctttac tgaaatggcg cgtttgcagg aaagcggtag tcctatcgat ctgattaccc
     4861 ttgcggaatc gctggaacgc caggggcaac tcgatagcgt cggtggtttt gcttatctgg
     4921 cagagttgtc aaaaaatacg ccaagtgctg cgaacatcag ttcctatgcg gacatcgtgc
     4981 gtgaacgtgc cgttgtccgt gagatgatct cggttgcgaa tgagattgcc gaagctggtt
     5041 ttgatccgca ggggcgtacc agcgaagatc tgctggatct ggctgaatcc cgcgtcttta
     5101 aaattgccga aagtcgtgcg aacaaagacg aagggccgaa gaacatcgcc gatgtgctcg
     5161 acgcaaccgt ggcgcgtatt gagcagttat ttcagcagcc acacgatggc gttaccgggg
     5221 taaacaccgg ttatgatgat ctcaacaaaa aaaccgctgg cttgcagccg tcggatttga
     5281 tcatcgtcgc cgcgcgtccg tcgatgggta aaacaacatt tgcgatgaac ctcgtcgaaa
     5341 acgcggcgat gttgcaggat aaaccggtac ttattttctc gctggagatg ccatcagaac
     5401 agatcatgat gcgttctctg gcgtcgctgt cgcgcgttga ccagactaaa atccgtaccg
     5461 ggcagctcga tgatgaagac tgggcgcgca tttccggcac catgggtatt ttgctcgaaa
     5521 aacgcaatat ctatatcgat gactcctccg gcctgacgcc aacggaagtg cgttcccgcg
     5581 cacgccgtat tgcccgtgaa cacggcggca tcgggcttat catgatcgac tacctgcaac
     5641 tgatgcgcgt accggcactt tccgataacc gtacgctgga aattgcagaa atctcccgct
     5701 cgctgaaagc actggcgaaa gaactgaacg tgccggtggt ggcgctgtcc cagttgaacc
     5761 gttctctgga acaacgtgcc gacaaacgtc cggtcaactc cgacctgcgt gaatctggct
     5821 ctatcgagca ggatgcggac ttgatcatgt ttatctatcg tgatgaggtg tatcacgaaa
     5881 acagtgattt aaaaggcatc gcggaaatta ttatcggtaa acaacgtaac ggcccaatcg
     5941 ggacggtacg cctgaccttt aacggtcaat ggtcgcgctt cgacaactat gcggggccgc
     6001 agtacgacga cgaataataa ttattttatg aattaggtaa ttaaagcaaa cacttatcaa
     6061 ggaacacaaa tgcaagcggc aactgttgtg attaaccgcc gcgctctgcg acacaacctg
     6121 caacgtcttc gtgaactggc acctgccagt aaaatggttg cggtggtgaa agcgaacgct
     6181 tatggtcacg gtcttcttga gaccgcgcga acgctccccg atgctgacgc ctttggcgta
     6241 gcccgtctcg aagaagctct gcgactgcgt gcggggggaa tcaccaaacc tgtactgtta
     6301 ctcgaaggct tttttgatgc cagagatctg ccgacgattt ctgcgcaaca ttttcatacc
     6361 gccgtgcata acgaagaaca gctggctgcg ctggaagagg ctagcctgga cgagccggtt
     6421 accgtctgga tgaaactcga taccggtatg caccgtctgg gcgtaaggcc ggaacaggct
     6481 gaggcgtttt atcatcgcct gacccagtgc aaaaacgttc ggcagccggt gaatatcgtc
     6541 agccattttg cgcgcgcgga tgaaccaaaa tgtggcgcaa ccgagaaaca actcgctatc
     6601 tttaatacct tttgcgaagg caaaccaggt caacgttcca ttgccgcgtc gggtggcatt
     6661 ctgctgtggc cacagtcgca ttttgactgg gtgcgcccgg gcatcattct ttatggcgtc
     6721 tcgccgctgg aagatcgctc caccggtgcc gattttggct gtcagccagt gatgtcacta
     6781 acctccagcc tgattgccgt gcgtgagcat aaagtcggag agcctgtcgg ttatggtgga
     6841 acctggataa gcgaacgtga tactcgtctt ggtgtagttg cgatgggcta tggcgatggt
     6901 tatccgcgcg ccgcgccgtc cggtacgcca gtgctggtga acggtcgcga agtgccgatt
     6961 gtcgggcgcg tggcgatgga tatgatctgc gtagacttag gtccacaggc acaggataaa
     7021 gccggggacc cggtcatttt gtggggtgaa ggtttgcccg tagaacgtat cgctgaaatg
     7081 acgaaagtaa gcgcttacga acttattacg cgcctgactt caagggtcgc gatgaaatac
     7141 gtggattaat cgttctgtaa tatttgattg tctgtgccgg atgcggcgtg aatgccttat
     7201 ccggccaata aaatcctacc aattcaataa gttgatattc tttcatgctc ttataaaggt
     7261 cgtgcctctg gcggatgtac gtttgtcatg agtctcactc tgttgctaat tgccgttcgc
     7321 ccctgaacat ccactcgatc ttcgccttct tccggtttat tgtgttttaa ccacctgccc
     7381 gtaaacctgg agaaccatcg cgtgtttcaa aaagttgacg cctacgctgg cgacccgatt
     7441 cttacgctta tggagcgttt taaagaagac cctcgcagcg acaaagtgaa tttaagtatc
     7501 ggtctgtact acaacgaaga cggaattatt ccacaattga aagccgtggc ggaggcggaa
     7561 gcgcgcctga atgcggtgcc tcatggcgct tcgctttatt taccgatgga agggcttaac
     7621 agctatcgcc atgccattgc gccgctgctg tttggtgccg accatccggt actgcaacaa
     7681 cagcgcgtag caaccattca aacccttggc ggctcagggg cattgaaagt gggcgcggat
     7741 ttcctgaaac gctacttccc ggaatcaggc gtctgggtca gcgatcctac ctgggaaaac
     7801 cacgtagcaa tattcgccgg ggctggattc gaagtaagca cttacccctg gtatgacgaa
     7861 gcgactaacg gcgtgcgctt taatgacctg ttggcgatgc tgaaaacatt acctgcccgc
     7921 agtattgtgt tgctgcatcc atgttgccac aacccaacgg gtgccgatct cactaatgac
     7981 cagtgggatg cggtgattga aattctcaaa gcccgcgagc ttatcccatt ccttgatatt
     8041 gcctatcaag gatttggtgc cggtatggaa gaggatgcct acgccattcg cgccattgcc
     8101 agcgctggat tacccgctct ggtgagcaat tcgttctcga aaattttctc cctttacggc
     8161 gagcgcgtcg gcggactttc tgttctgtgt gaagatgccg aagctgcagg ccgcgtactg
     8221 gggcaattga aagcaacagt tcgccgcaac tactccagcc cgccgaattt tggtgcgcag
     8281 gtggtggctg cagtgctgaa tgacgaggca ttgaaagcca gctggctggc ggaagtagaa
     8341 gagatgcgta ctcgcattct ggcaatgcgt caggaactgg tgaaggtatt aagcacagag
     8401 atgccagaac gcaatttcga ttatctgctt aatcagcgcg gcatgttcag ttataccggt
     8461 ttaagtgccg ctcaggttga ccgactacgt gaagaatttg gtgtctatct catcgccagc
     8521 ggtcgcatgt gtgtcgccgg gttaaatacg gcaaatgtgc aacgtgtggc aaaggcgttt
     8581 gctgcggtga tgtaatgcag gaaagcaggc tggaattacc cagcctgcag tgaaattaaa
     8641 ctgtcgtcgc tttcactctt tctttataga tgattttttt gataccatcg ttctacgtga
     8701 gagataataa acgttgttag ttcttttat
//
LOCUS       DAZFDC010000162         8580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFDC010000162 DAZFDC010000000
VERSION     DAZFDC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.162"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHJ48_004213"
     CDS             complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHJ48_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO4709652.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            573..2771
                     /gene="priA"
                     /locus_tag="ACHJ48_004214"
     CDS             573..2771
                     /gene="priA"
                     /locus_tag="ACHJ48_004214"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO4709653.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2927..3952
                     /gene="cytR"
                     /locus_tag="ACHJ48_004215"
     CDS             2927..3952
                     /gene="cytR"
                     /locus_tag="ACHJ48_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO4709654.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHJ48_004216"
     CDS             4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHJ48_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO4709655.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5096..5626
                     /gene="hslV"
                     /locus_tag="ACHJ48_004217"
     CDS             5096..5626
                     /gene="hslV"
                     /locus_tag="ACHJ48_004217"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO4709656.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5636..6967
                     /gene="hslU"
                     /locus_tag="ACHJ48_004218"
     CDS             5636..6967
                     /gene="hslU"
                     /locus_tag="ACHJ48_004218"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO4709657.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7034..7960
                     /gene="menA"
                     /locus_tag="ACHJ48_004219"
     CDS             7034..7960
                     /gene="menA"
                     /locus_tag="ACHJ48_004219"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO4709658.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            8053..8538
                     /gene="rraA"
                     /locus_tag="ACHJ48_004220"
     CDS             8053..8538
                     /gene="rraA"
                     /locus_tag="ACHJ48_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO4709659.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2087 a         2222 c         2345 g         1926 t
ORIGIN      
        1 taaaatcctt ttgaaataaa taagaaccag aagcctagcg ccagtcaaca acaggaaatg
       61 ataatgagtt aacaccccgg aatttttcag aatttataaa aggcaaatta caggcgcaaa
      121 aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta tttgctgccc gggatgttga
      181 aacgcttgtt gaagcggtca acacggccac cggtagcaac atcacgctgt ttgccagtga
      241 agaacgggtg gcacttgctg cacacgtcga ggttcaggtc atgaccaacg gtggagcgga
      301 ttttcattac gttaccgcaa gagcagctag cagtaatttc ttcgtatttc gggtgaatat
      361 cttttttcat gggaaaacct cggtttaagg ccgcgtcgct cttccagccc taacgccaga
      421 caccacgcga tgttaaaagt atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc
      481 atacagaaat taaccagcat atgcaaactg atccgcactc ttctacggca atgtgtatac
      541 taacccaccg aatttcaagt caggatgatg ctatgcccgt tgcccacgtt gccttgcccg
      601 ttccgcttcc tcgtaccttt gactatctcc tgccagaagg catggcggtt aaagccgggt
      661 gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat cggggttgtg gtgtcagtta
      721 gcgacgtcag tgagctgccg cttaacgagc taaaagcggt agtcgaagtg ctggatagtg
      781 agccggtgtt tactcactcc gtctggcgat tgctgctctg ggcggcggat tactatcatc
      841 atccgattgg cgatgtgctg tttcatgcct tgccgatttt actgcgccag gggcggcctg
      901 cggcgaacgc gccgatgtgg tactggtttg ccactgaaca aggccatgcg gtggatctga
      961 acagcctgaa acgttcgcca aagcaacaac aggcactggc ggcattgcgg caaggcaaaa
     1021 tctggcgcga ccaggttgcc acgcttgaat ttaatgatgc cgcgttacag gcgctgcgca
     1081 aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt tagcgactgg cgaacgaact
     1141 atgccgtttc tggtgaacgg ttgcgattga ataccgaaca ggccaccgcc gttggcgcaa
     1201 ttcatagcgc ggcggatacc ttctctgctt ggctgctggc gggcgttaca ggttccggta
     1261 aaacagaggt ttatttgagc gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg
     1321 tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta
     1381 atgcccccgt agaagttctg cattccggcc tgaacgacag cgagcgtctt tcggcgtggt
     1441 tgaaagcgaa aaatggtgag gcggcgattg tcatcggcac ccgctccgcg ctgtttacgc
     1501 cgtttaaaaa tctcggtgtg attgtcattg atgaagagca cgacagttcc tacaagcagc
     1561 aggaaggctg gcgctatcat gcccgcgacc tggcggttta tcgtgcgcac agcgagcaaa
     1621 tcccgattat tcttggctcc gcaacacccg cactggaaac gttatgcaac gtccagcaga
     1681 aaaaataccg cctgctgcgc ctgacgcgtc gggcggggaa tgcgcgtccg gcaattcaac
     1741 atgtgctgga tttaaaaggt cagaaggtgc aggcggggct ggctccggcg ttaatcaccc
     1801 gtatgcgcca gcatttacag gccgacaacc aggttatcct ctttcttaac cgccgtggct
     1861 ttgcgcctgc cttgctgtgc cacgactgcg gctggattgc cgaatgtcca cgttgcgatc
     1921 actactacac gctgcatcag gcgcaacagc atttgcgctg tcaccactgt gacagccaac
     1981 gcccggtgcc gcgccagtgc ccttcctgcg gttccacgca cctggtaccc gtggggctgg
     2041 gcaccgaaca gcttgaacag acgctcgcgc cgttattccc tggtgtgccc atttctcgta
     2101 tcgaccgcga taccaccagc cgcaaaggag cgctggaaca acaactggcg gaagtgcatc
     2161 gcggcggcgc gaggatattg attggcacgc aaatgctggc gaaaggtcac cactttccgg
     2221 atgtgacgct ggtagcgtta ctggatgtgg acggcgcgct gttttctgcc gattttcgct
     2281 cggcagagcg ttttgcccag ctatacaccc aggtagcagg ccgtgccggg cgtgcgggta
     2341 aacagggcga agtggtgctg caaacgcatc atccggaaca cccgctattg caaacgttgc
     2401 tctataaagg ctacgacgct tttgccgaac aggcactggc tgagcggcga atgatgcaac
     2461 tgccgccgtg gaccagccat gtgattgtgc gtgcggaaga tcataacaat cagcacgcgc
     2521 cattgttcct gcaacaactg cgtaatctga tcctctccag cccgctggca gacgacaaat
     2581 tgtgggttct cggtccggtt ccggcactgg cacctaaacg tggcggtcgc tggcgctggc
     2641 agatattgtt gcagcaccct tcccgcgtgc gcttgcaaca catcattaac ggtacgctgg
     2701 cactcatcaa tacaataccg gattcccgta aggtgaaatg ggtgctggat gttgatccga
     2761 ttgagggtta aaccgctcac gatgcgaggc ggatcgaaaa attcaatatt catcacactt
     2821 ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa
     2881 gtaaacatgg atgtagtacg cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa
     2941 gcaggaaact gccgcgacca tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac
     3001 cgtctcccga gcattaatga atcccgataa agtctcccaa gccacccgta atcgggttga
     3061 aaaagcggcc cgtgaagtgg gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa
     3121 tgaatcccgc accattctgg tgattgtccc ggatatctgc gatcccttct ttagcgaaat
     3181 tattcgcggt atcgaagtta cggcggcaaa tcacggatat ctggtgctga ttggcgactg
     3241 tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg atcatcacca agcaaattga
     3301 tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc agcattgagg aacagcgtaa
     3361 tctgccgccg atggtgatgg cgaacgaatt tgcaccggaa ctggagctgc ctaccgttca
     3421 tatcgacaat ctgaccgccg catttgatgc agtaaattat ttatatgagc aagggcataa
     3481 acggattggc tgtatagccg gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg
     3541 ttacgttcag gcgctgcgtc gctgcggcat tatggttgat ccgcaataca tcgcccgtgg
     3601 cgacttcacc ttcgaagccg gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc
     3661 gcctactgct gtcttctgcc atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa
     3721 acgccagggg ctgaaagtcc cggaagacct ttccataatc ggttttgata acatcgacct
     3781 gacgcaattt tgtgatccgc cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg
     3841 ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa cacgttggca gtggctctcg
     3901 tttaatggac tgcgaactta tcatccgggg atcaacacgc gcgttacctt aaagtaaacc
     3961 tttaagactg ccgtgactgg tcaaaggccc gccgcttaag taacatggcg ggctgacgaa
     4021 cgaataaata cagcgaaacg atagtggcac aacgagatta tgtacgccgc agccaaccgg
     4081 caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca acgaaatctg cctgcggttt
     4141 ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac ctttatcggt ggtctgtact
     4201 tcattacgca tcacaagaaa gaagagtccg agacgctgca aagccagaaa gtgaccggaa
     4261 acggactacc accaaaacca gaagaacgct ggcgctacat caaagagctg gaaagtcgcc
     4321 agccgggtgt gcgtgcgccc acagaacctt ctgccggtgg tgaagtgaaa acgccggagc
     4381 aactgacacc agaacaacgt cagcttcttg aacaaatgca ggctgatatg cgccagcagc
     4441 caacgcagct ggttgaagtt ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc
     4501 tacagcgcca acgtcaggcg cagcaattag cggaacagca acgcctggct cagcagtcgc
     4561 gtacgactga acaaagctgg cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg
     4621 cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa
     4681 cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc tgcgccggtt actcgtgccg
     4741 ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg
     4801 gttcgttcag aggcgcggaa caggcagaga cggtacgtgc tcagctggcg ttcgaaggct
     4861 ttgactcaaa aattaccacc aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag
     4921 gcaaagagaa cgcagacagc accctcaatc ggttgaagat ggcgggtcat acaaactgca
     4981 ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc ccatctataa ttgcattatg
     5041 ccccgtactt ttgtacgggg tttgtactct gtattcgtaa ccaaggggtc agctcgtgac
     5101 aactatagta agcgtacgcc gtaacggcca tgtggtcatc gctggtgatg gtcaggccac
     5161 gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa
     5221 agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt acgctgttcg aactgtttga
     5281 acgtaaactg gaaatgcatc agggccatct ggtcaaagcc gccgttgagc tggcaaaaga
     5341 ctggcgtacc gatcgcatgt tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac
     5401 tgcatcgctt atcatcaccg gtaacggtga cgtggtgcag ccagaaaacg atcttattgc
     5461 tatcggctcc ggcggccctt acgcccaggc tgcggcgcgt gcactgttag aaaatactga
     5521 acttagtgct cgtgaaattg ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta
     5581 taccaaccat ttccacacca tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc
     5641 tgaaatgacc ccacgcgaaa tcgtcagcga actggataag cacatcatcg gccaggacaa
     5701 cgccaagcgt tccgtggcga ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga
     5761 agagctgcgc catgaagtga ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg
     5821 taaaactgaa atcgcccgtc gtctggctaa gctggcgaat gcgccgttca tcaaagttga
     5881 agcgaccaaa ttcaccgaag tgggctacgt cggtaaggaa gtggattcta ttattcgcga
     5941 tctgaccgat gccgccgtga aaatggtacg cgtccaggct atcgagaaaa accgttatcg
     6001 cgctgaagaa ctggcagaag aacgtattct cgacgtgctg atcccacctg ctaaaaacaa
     6061 ctggggacag accgaacagc agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa
     6121 actgcgtgaa ggccagcttg atgacaaaga aatcgagatc gatcttgccg cagcaccgat
     6181 gggcgttgaa attatggctc ctccaggcat ggaagagatg accagccagc tgcagtccat
     6241 gttccagaac ctgggcggtc agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat
     6301 gaagctgctg attgaagaag aagcggcgaa actggtgaac ccggaagagc tgaagcaaga
     6361 cgctatcgac gctgttgagc agcacgggat cgtgtttatc gacgaaatcg acaaaatctg
     6421 taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa ggcgttcagc gagacctgct
     6481 gccgctggta gaaggttgca ccgtttccac caaacacggg atggtcaaaa ctgaccacat
     6541 tctgtttatc gcttctggcg cgttccagat tgcgaaaccg tctgacctga tcccggaact
     6601 gcaaggtcga ctgccaatcc gcgttgaact gcaggcactg accaccagcg acttcgagcg
     6661 tattctgacc gagccgaatg cctctatcac cgtgcagtac aaagcactga tggcgactga
     6721 aggcgtaaat atcgagttta ccgactccgg tattaaacgc atcgcggaag cggcatggca
     6781 ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta cacactgttc tggagcgttt
     6841 aatggaagag atttcctacg acgccagcga tttaagcggt caaaatatca ctattgacgc
     6901 agattatgtg agcaaacatc tggatgcgtt ggtggcagat gaagatctga gccgttttat
     6961 cctataatcg cgttcaatca ttttcatcat tgtttgatgg ggctgaaagg ccccattttt
     7021 attggcgcgt attatgactg aacaacaaat tagccgaact caggcgtggc tggaaagttt
     7081 acgacctaaa accctccccc tcgcctttgc tgcaattatc gtcgggacgg cgctggcatg
     7141 gtggcaaggt cattttgatc cgctggtcgc cctgctggca ctgattaccg ccgggctatt
     7201 acagatcctt tctaacctcg ccaatgatta cggcgatgcg gtaaaaggca gcgataaacc
     7261 tgaccgtatc ggaccgctac gcggcatgca aaaaggggtc attacccagc aagagatgaa
     7321 acgggcgctc attattaccg tcgtgctcat ctgtctctcc gggctggcac tggttgcagt
     7381 ggcgtgccat acgctggccg attttgtcgg tttcctgatt cttggcgggt tgtcgatcat
     7441 tgccgctatc acctacaccg tgggcaatcg tccttatggt tatatcggtc tgggtgatat
     7501 ttccgtactg gttttctttg gctggttaag cgtcatgggg agctggtatt tacaggctca
     7561 tacattgatt ccggcactga tccttccggc gaccgcatgc ggcctgctgg caacggcagt
     7621 actgaacatt aataacctgc gtgatatcaa tagcgaccgc gaaaacggca aaaacacgct
     7681 ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc
     7741 tctggtatgt ctggcgctgt ttaatctctt ttcgctgcat agcctgtggg gctggctgtt
     7801 cctgctggcg gcaccgttgc tggtgaagca agcccgttat gtgatgcggg aaatggaccc
     7861 ggtggcgatg cgaccgatgc tggaacgcac tgtcaaggga gcgttactga ctaacctgct
     7921 gtttgttgta gggatattcc taagccagtg ggcagcataa ctgacaaata tcaattaaca
     7981 attgatgatt ttgccaacag accacatagc gcgatatact gaaaattctc gcagcaactg
     8041 aatgttaagc ctatgaaata cgatacttcc gagctttgtg acatctatca agaagatgtt
     8101 aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg cgtcgtttgg cggacaaata
     8161 atcacggtaa aatgtttcga ggacaacggg ttgctgtacg atctgctcga acagaatggc
     8221 cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct
     8281 gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg
     8341 cgtcaggtag atgacctgga agagttggat atcggcatcc aggcgatggc ggcaattccg
     8401 gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtt
     8461 accttcttct ccggcgacca tctttatgcc gacaataccg ggattattct ttcagaagat
     8521 ccgctggata ttgaataagt atctgccatg cgttaaaagg ggaattaaat tccccttttt
//
LOCUS       DAZFDC010000163         8499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFDC010000163 DAZFDC010000000
VERSION     DAZFDC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.163"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(28..215)
                     /locus_tag="ACHJ48_004221"
                     /pseudo
     CDS             complement(28..215)
                     /locus_tag="ACHJ48_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(335..1117)
                     /locus_tag="ACHJ48_004222"
                     /pseudo
     CDS             complement(335..1117)
                     /locus_tag="ACHJ48_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1833..2291)
                     /locus_tag="ACHJ48_004223"
     CDS             complement(1833..2291)
                     /locus_tag="ACHJ48_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4709660.1"
                     /translation="MTLRKLFGQRVKELRIATGMSQEAFADRCGFARSYMSRIERGGS
                     NASLDAIEVLANALSVEPWRLLVSDSSEDSDPELLVPYAADGSCFHPGLASTRDGSFG
                     VGDKTAQKRFGTFSEALEYLRSMETAKWRRPNPSGNWGIVSAVRWDKLRK"
     gene            2414..3187
                     /locus_tag="ACHJ48_004224"
     CDS             2414..3187
                     /locus_tag="ACHJ48_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709661.1"
                     /translation="MSKTRLSKPGFSAKSVLTIMLIAASSASYAAESIDQLATRIGMD
                     HFREAIKKGERSAFKTLPAKGYKNGLTGLLIKTNTYVNPEYSFADCDTDTVIISVQEH
                     KTLPGCRISLKLATKDADGSYHDNGIRVEYGIAIGSKKFIANNSAWAQHAISGDKVLE
                     GALHPDKTNNNKDQMNNNKDQMCMGMAQFTANVTEGRNSGMTKREYYQRLDNMNGKAD
                     VTQQMITLYKTFVDYVYEHPKQDAKISGTYIYTNCMNNF"
     gene            3339..4655
                     /locus_tag="ACHJ48_004225"
     CDS             3339..4655
                     /locus_tag="ACHJ48_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709662.1"
                     /translation="MNDGVMLDSDVIAGLEWLASTQENKQHFYQRLAKAQQFYIATTQ
                     KAANFGKQFDPEWYGSDVVAGYFAQAKSLIDNRRSYEVTNASNIIPWVKQLGVCAKSL
                     DRITGARDRAIRMLKNTTVLPDTALLELVLAGNYASEGYEVEFIPEQKGIAKTPEFRC
                     RLGDGDYFFVECKRLQKGPYVKQEILQHHIRSHLAELHIKSKKMNVWTDVTYLCEVKD
                     APENYIISHLKNFKGVFYEWNDDYGKGTIRPANLNLIREDITENGSLLVNTKLARLIK
                     GSPLEDENYQVVAMGRPDERDSRFITKVKLASLITWRCINEKSFDARSRHVTKTLAEI
                     DNQLLDYGLGVGHIALDVDIQKDVADKRREKNYAAIVNFEQKSKIVRLNVHYLVPRID
                     ENSAWMVDETADDFFTHDLVKYVIPLTKIFPEAEVFENDQPGWHQN"
     gene            complement(4854..5423)
                     /locus_tag="ACHJ48_004226"
     CDS             complement(4854..5423)
                     /locus_tag="ACHJ48_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO4709663.1"
                     /translation="MAIFGYGRVSTSQQDTENQRMELEQAGWTFDFWFSDVVSGKVPA
                     VQRKAFFEMLSKIRDGETLVVAKLDRLGRDAIDVLQTVRTLADRNIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTNAGLLRAKAEGRKLGRPAKIAPEARGAILDK
                     RAAGVSVSALAREYGVSRATLAALLNKGY"
     gene            5616..6083
                     /locus_tag="ACHJ48_004227"
     CDS             5616..6083
                     /locus_tag="ACHJ48_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFO4709664.1"
                     /translation="MIKDAIFSPCGKYRYSLSRVWDESKPYTLFIGLNPSYADAEKDD
                     RTLSRCISFAKSWGYGGVYMANLFAFVHTQRHEMMKASDPIGKDNDSHLIRLVSGAGL
                     VVAAWGNEGRHLKRSTTVRQLLPESTMCFVLNATGEPKHPLYMKNDSVLIPLG"
     gene            7087..7419
                     /locus_tag="ACHJ48_004228"
     CDS             7087..7419
                     /locus_tag="ACHJ48_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HFO4709665.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSHGIDVVSFGNS
                     LDDLDCYYLIRAFDSAKSMTAVLDAFYASADWRSGPREDIIGSIENCIKTVISLPSES
                     VKGLRMQS"
     gene            7541..7918
                     /locus_tag="ACHJ48_004229"
     CDS             7541..7918
                     /locus_tag="ACHJ48_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HFO4709666.1"
                     /translation="MNLDEEFYELQKLFAQKDLLTEKRRSSGGGFMEILLVKRQNMKI
                     KIYQEKGHQLPHIHIDYGKQAHAASYAIQSGDRIEGDLPKKYDSDVSSWLGRNRDKIL
                     EIWNALQAGAPYEPLIAELTGGV"
     gene            complement(8101..>8499)
                     /locus_tag="ACHJ48_004230"
     CDS             complement(8101..>8499)
                     /locus_tag="ACHJ48_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO4709667.1"
                     /translation="VSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTER
                     LKEAKLLASTESTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNN
                     RRLLGRLGHIPPAEAEKAYYASIGNDDLAA"
BASE COUNT         2431 a         1967 c         1892 g         2209 t
ORIGIN      
        1 ccaaataatg agatcagcaa acatctattt gaaacgttgg ttctcatcct caagatcctt
       61 gattatttta atatcagaaa attccatacc gccgtatctg gacttgcagt tgtagtaagt
      121 ggcttcagat aagccacatc tttaacagtt cgcccgtatt caagcgattt aatcacggcg
      181 ataatctgat gctcagtaaa aggggcttta cgaatagcga tctccttcat tggcagattg
      241 attatgccgg aggatctcta aatgtgaatg gcacgattat gcgggatatt tactgtacca
      301 gtccaaccct ctgcttactg caataccaat caagagggat ggtccacatt gacgtttcaa
      361 aatcgatttc tgaccagcga gcaaaatgca gcttactgga ccgaataaag ataagcagag
      421 tgagttctgt cgtacagtgg gttagcggtc tgccggtgta gctgtctatt ttgtcaagca
      481 actcagggat acgctttaac tccagcgccg gacgatgctg tcgattactg gaagcaaccg
      541 cccccgacat ctcttgcgcc ggatcgtaat caattaaccc gctttgcact gcataacgca
      601 tgatggccgt ggtgcgctgt taaaggcggg aagccacttc aagacgcccg gatttctcca
      661 cggctttaat aggaattaac agatcacggg tgcctagttc agcaatgttg cgagtaccaa
      721 ttgtcgggaa gatattgtcc tccaggctct tttttacacg attagcatga tcttccgacc
      781 atttttggtt ggttacgagc cactctctgg caaccttctc gaaagtaata atctctttcg
      841 cctgctcttc tttcacagca cgcttatgct cacgaggatc gataccagca gctaccagct
      901 ttctggcctc ttcccttttc ttccgcgcat ccgccagtga aacatccggg taaacgccga
      961 acgccatcaa atgttgttta ccgccaaaac ggaaacgaaa tcgccaatac tttgaaccgc
     1021 caggttttac aagtaaggac atgccgtcgc cgtcaacaag tgaatactct ttctcctcag
     1081 gtttcgcaga gcgaacttta gtatccgtta gggccataat ggtcttcctt ccattggtat
     1141 ctcgtgagta tacaagcaat atcgaatcgg caaatatact cattagtata ctcacaagcc
     1201 tgttgatgtg ggttggttca ggttgacttc agttgaggag aaaagctgga aaagccttgc
     1261 gtggcgcgga tttcaggcat aaaaaaagac ctcagttgag gtctatttac atacttttgg
     1321 tgccgaaggc cggactcgaa cctaagtctc cataacctgc tgtaatcacg ttcctattag
     1381 cctttaaaag aacaatcgta ggaccttttt gttctagggg ctacatctta agtttgatcc
     1441 gctggacgga atcaaaccga gtgttcgtgg cggcacaaat cattaacgtg ccatacctgc
     1501 cttttgatgt ttatggaggt ctagtgggtc gggaggaaag aacatttgtc agctatgtgg
     1561 atgttaagta aacgccactg cgttcggatt ttgaaaccag ttaaaccccg tcagaccttg
     1621 ttcagaataa ctatttaact caatgttatt tatatgaatt aagccattca gcgtcaaagt
     1681 aaaacgccac cgtaacgcct acgtaacgca cccgcatttt gctgttttat tcactcttat
     1741 agaaacaaag agctgatgaa acgtgaggct atcagcatct aatgaacact gttcatccat
     1801 acagcatgaa aattcataaa cttacacttg agctactttc tcagcttgtc ccaccgaacc
     1861 gcagatacaa taccccagtt gccgctagga ttaggcctac gccattttgc tgtctccatg
     1921 ctacgaagat actcaagagc ttcagaaaac gtgccaaacc gcttctgagc cgttttatca
     1981 cctacgccaa acgagccatc gcgagtgctt gccagcccgg gatgaaaaca cgagccatca
     2041 gctgcataag gaaccagtaa ttccgggtca ctatcttcag atgaatcaga caccaatagt
     2101 cgccacggct caacactcag agcattagcc agcacctcaa tcgcatccaa agacgcatta
     2161 gagccaccgc gttcaatgcg gctcatatag ctacgagcaa aaccacaccg atcggcaaag
     2221 gcttcttggc tcattccggt ggcaatgcgc aactctttta ctcgttgtcc aaacagtttt
     2281 ctcagggtca ttttcatgac ctgaatttgc gcagatgtcc actaaagagc cacgctccaa
     2341 tagtgacatt ttgaatcaat ggtctatgat gcgataactg acaccgctgg ggtggattct
     2401 gagggataac aacatgtcaa aaacacggct tagtaagccc ggtttttcag ccaagtctgt
     2461 gctgaccatt atgttaatcg ctgcgtcatc tgcatcttat gcagcagaaa gcatcgatca
     2521 actggcgacg cgcatcggta tggatcactt ccgggaagcg atcaaaaagg gtgaacgcag
     2581 cgccttcaaa acgctgccgg caaaaggcta taaaaacggt cttaccgggc tgttaatcaa
     2641 aactaacact tacgttaacc ccgaatacag ctttgctgat tgcgatacgg acaccgttat
     2701 tatctcggtg caagaacaca aaacgcttcc cggttgccgt atatctttaa agctagcgac
     2761 aaaagacgca gatggcagtt atcacgacaa tggaattcgc gttgaatacg gtattgccat
     2821 cggctccaaa aagtttatcg ctaacaattc agcgtgggcg caacatgcca tcagcggtga
     2881 taaagtgttg gaaggcgctc tccacccgga taaaacgaat aacaataaag atcaaatgaa
     2941 taacaataaa gatcaaatgt gcatgggtat ggctcaattt accgcaaacg taaccgaagg
     3001 ccgtaacagt gggatgacga aaagagaata ttatcagcga cttgataaca tgaacggtaa
     3061 ggccgacgtc acccagcaga tgataacgct ctataagact tttgttgact acgtatatga
     3121 acatccaaag caagacgcaa agatttcagg gacgtatatt tatactaact gtatgaacaa
     3181 cttctagaaa cgtaggtgcc tgaacatgat agaactcagc tgccagcata gagtggaaag
     3241 cggaggctta ccatcgtata ttcatcgcgt tatctttccg taactataat ctgtctcagt
     3301 tgatctagtg cctttcaggg ctgaactaag gaataaagat gaatgacggt gttatgctag
     3361 attctgacgt tattgctggt cttgagtggt tagcatcaac tcaagagaac aaacaacact
     3421 tttaccagcg cttagcaaaa gcacagcaat tttacatagc tacaacccaa aaagctgcca
     3481 atttcgggaa acagtttgac ccagaatggt atggttccga cgtggttgct ggttatttcg
     3541 cacaagcaaa atcgcttatc gataatagaa ggtcttacga agttactaat gcttcaaata
     3601 taataccatg ggttaaacaa ttaggagtgt gtgctaaatc gctcgataga attactggcg
     3661 ctagagatcg cgcaatcaga atgcttaaga atacaacagt attaccagac acagccttat
     3721 tagagttggt tcttgctgga aactatgcat cagaaggtta tgaagttgag ttcattccag
     3781 aacaaaaagg gatagcaaaa accccggagt ttcgttgccg ccttggagat ggcgattatt
     3841 tctttgtcga atgcaaaagg cttcagaaag gtccttacgt aaagcaggaa atattacagc
     3901 atcatatacg ctcacatctt gccgaacttc atatcaaatc caaaaagatg aacgtatgga
     3961 ctgatgtcac atatttatgt gaagttaaag acgcgcctga aaactatatc attagccact
     4021 taaaaaactt taaaggtgta ttttatgaat ggaatgatga ttatggaaaa ggaacgatta
     4081 gaccggccaa cttaaatctt atcagggaag atatcactga aaacggctca ttactagtca
     4141 acactaaact tgctcgtctt atcaaaggct cccctctgga agatgaaaat taccaagttg
     4201 ttgctatggg acgaccagat gagcgcgact cacgtttcat aaccaaagtt aaacttgctt
     4261 ctttgataac atggcgttgt atcaatgaaa aatctttcga tgcacgttcg cgacatgtaa
     4321 caaaaacatt agcagagata gataaccagc ttttggatta tggtctaggc gtagggcata
     4381 tagctttgga tgtagatatt caaaaggatg ttgccgataa acgaagggaa aaaaactatg
     4441 ctgccatagt aaattttgag caaaaatcta aaatagtccg cctgaatgtt cattatctcg
     4501 tgccacgcat agatgagaac tctgcgtgga tggtagatga aactgcggat gactttttta
     4561 cccatgatct ggtcaaatat gttatcccat tgactaaaat attccccgag gctgaggtat
     4621 ttgagaatga ccaacctggc tggcatcaaa attaatttat ttttgatagt atgaacgagg
     4681 gtagcatttc tatacaaatc agtgttcggc tgatagcgag ttaaacagtt tactgaaatg
     4741 ttctcagcaa ttagctgttg ccaggacctc aaaaatccga ttctggcgga aagtgtgctg
     4801 ttttaccaag tattgctcgt atctgcaaag tgttagagca tgttttaagt atatcagtaa
     4861 cccttattca gtaatgcagc aagcgtcgct cttgaaacac cgtattcacg cgccaatgcg
     4921 ctaacactaa ccccagcggc ccttttgtct aaaatcgctc cacgagcttc cggtgcgatt
     4981 ttagcaggcc tgcccaattt cctgccttca gcttttgccc gtaacagccc cgcattagtc
     5041 cgttcaatca gcaggtcacg ctccatctcc gcaaccgctg ctagcatcga caacaggagc
     5101 ttaccggcag cagaggtgag atcagtggtg ccaagctgat ggacaataac cttgatgttt
     5161 cggtcagcca acgtcctgac agtttgcaga acgtcaattg catcacgccc gaggcggtcc
     5221 agctttgcaa ctaccaacgt ttccccatcg cggattttgc tgagcatttc aaaaaacgct
     5281 ttgcgttgca ccgcaggaac ctttccgctg actacatcag aaaaccaaaa gtcgaacgtc
     5341 cacccagctt gttcaagttc catgcgctgg ttctcagtat cctgctggga tgtcgaaaca
     5401 cgcccgtagc caaaaatagc catagtcatc tcctcatccg gcccctgcca gaaaacggtg
     5461 atgaaaatag gatggcctgc cggaaaagcc aaggctaatt ttccaacagt gtgaaagcgt
     5521 gcggttggag gatgccggaa aacggtcgtt tgttggcgct taaaacgggt gaagacatgt
     5581 taggctgaaa ccatcacttc ataaggacaa ccgaaatgat taaagacgcg attttttcac
     5641 cgtgcggtaa gtatcgctac tcgttatcac gagtctggga cgaatcaaaa ccgtataccc
     5701 ttttcatagg gctaaaccct tcatacgccg atgccgagaa ggatgatcgc accttgagcc
     5761 gctgcatatc gtttgccaaa agctggggct acggtggcgt atacatggcc aacctgttcg
     5821 cattcgttca cacccagcga catgaaatga tgaaagcgtc tgatcccatt ggcaaagata
     5881 acgatagcca cttgatacgt cttgttagcg gtgccggact ggttgtcgcc gcatggggaa
     5941 atgaaggaag acacctcaaa cgctcaacga ccgtccgaca gctattgcca gagagcacaa
     6001 tgtgcttcgt cttaaacgct acaggtgagc cgaagcaccc tttgtatatg aaaaacgaca
     6061 gtgttctcat acctttaggt taacatcgat aactgcgttc agaatatgaa cgcagccata
     6121 tgatatatat gacttttctt taaaatgatg gtgtgctagc ttctctcctg acacctacct
     6181 catgcggtaa accggtccgg gtaggtggag atcaccgctg tttagcggaa gacgtcagga
     6241 tgggaactct gcgtaacgtt gattgtggta ggtagggcgc gcaagcgttt gatgcagcca
     6301 agcagagggt gaaagtagct atcggcggat tcttaagtag cttcttttgc gcataatgca
     6361 tctttcaaga aaggtcgtca caccacgagg aagacttgat cttcatctgg caaggggaac
     6421 cgagcaaacc tacaggctat cttctcagat ggccttaggg ttgatatgtc cggcttcccc
     6481 cccgaggaag ggtttacatc ttaaagaaca agttatgttt taacattttt aacaattaca
     6541 cttctctgct gtttggtgtc ttatggcgtt cccgtcaaaa gtaaagctgc cgtttttacc
     6601 tcacatgcgt aacaaccaaa cacgaaccag aagctgctaa cgttcaacgt gttcgcggtg
     6661 taaatgcagc cggataagtt gggagcgaaa aagtggatgt cctaggtttg gaagcgcccc
     6721 tcttaagcat tgcatccgat aatttccgtg gcggtgagac agtccttata gaggcaatca
     6781 gcctccaaag ttaagcgacg cggtaaaact aaagctcgcc cagatttctc attgattgct
     6841 cgaactccgc tacgcttgtt tcaactggct tcaccagtca ctctatgaga gcgccgcgaa
     6901 ttctgcacca tgatttcgac taccttcttt aaaacactcg ccagagagcc ttacagcgcg
     6961 tttgaaccgc atatcgcagc ttctgcttac ggatgactgt ctgattagca gtagcttcat
     7021 ggcccagtcc ggtttacttt attgctatca actaggagcc gcccaagggc aacacggaga
     7081 taacgcttga gaaccgtcga aatactgcaa tacaccttac gcaaaggcag cggtgccgct
     7141 tttcacgcca taatgcagga aatcagtgtt cccctacacc aaagccacgg gatcgatgtt
     7201 gtttcgtttg gcaactcgct tgatgacctg gattgctact acttgattcg cgcctttgac
     7261 agcgctaaaa gcatgactgc ggttctcgat gctttttacg caagcgcaga ctggcgcagc
     7321 ggcccacgcg aagacatcat tggcagcata gagaactgca ttaaaacagt gattagccta
     7381 ccatcagaaa gcgtgaaagg gctgcggatg caatcgtaag taatcaagtg tattagctta
     7441 gatttgacct aaacagcttt gtgccaaaag tggactagga aaagcctagc gataaagagt
     7501 tgagtttaat aactactaac tgaaatttaa tgggtgatat atgaatttag atgaagaatt
     7561 ttacgaatta caaaaactct ttgctcagaa ggatctactg actgagaaaa gacgctctag
     7621 tggcggcgga ttcatggaaa ttctcttggt gaagagacag aacatgaaga taaaaatata
     7681 tcaagaaaaa ggacatcaac ttccccatat tcatattgac tatggaaaac aagcgcatgc
     7741 tgccagttat gccatacaat caggagaccg catagaggga gatttaccta agaaatatga
     7801 tagtgatgtt tcgagttggt tggggagaaa tcgtgataaa attttagaaa tctggaatgc
     7861 actacaagct ggcgcacctt atgaaccact aatcgcagaa ttgactggcg gagtgtgact
     7921 aactaaataa ttttccccta aaaaaattcg atgtaggaaa atatatataa gtaagaatca
     7981 tgagtgtccg ctcctcgctc aaggcggact gttagacttg attgtatgct gtcagagaaa
     8041 accgtcagat caattctgtg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac
     8101 tcaggctgcc agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg
     8161 agggatatga cccagccttc ccagcaatcg tcgattgtta taccagtcca cccacgtgag
     8221 tgtggccagt tccacttctg cacggttttt ccagctctta cggtgtatta cctccgcttt
     8281 gtaaagacca ttgatgctct ccgccatcgc gttgtcatac gagtcacctg tactctctgt
     8341 tgatgccagc agttttgctt cttttagtcg ctccgtatag gccagtgata catactgaga
     8401 acctttatca ctgtgatgga ctgtgccgga cggccgacgg gcccacaacg cctgctccag
     8461 tgcatccagc acgaatgtcg tttccataga cgatgagac
//
LOCUS       DAZFDC010000164         8404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFDC010000164 DAZFDC010000000
VERSION     DAZFDC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.164"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..3799
                     /locus_tag="ACHJ48_004231"
     CDS             <1..3799
                     /locus_tag="ACHJ48_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO4709668.1"
                     /translation="LENNGIAITQANGEAHVTLKGKKAGTHTVTATLSNNNTSDSQPV
                     TFVADKTSALVVLQISKNEITGNGVDSATLTATVKDQFDNEVNNLPVTFSTASSGLTL
                     TPGESNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIELTP
                     VPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTNEQG
                     KATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALFDTV
                     SAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASFTAT
                     KAGVYQVTATLENGDSMQQTVTYVPNVANAEISLAASKDPVIANNNDLTTLTATVADT
                     EGNAIANSEVTFTLPEDVRANFTLGDGGKVVTDTEGKAKVTLKGTKAGAHTVTASMAG
                     GKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAVTFT
                     LPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVTLIA
                     DAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSSTSVE
                     TDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPEGQV
                     MVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMTPER
                     NGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANGTPV
                     EGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQTQTI
                     VKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKSGSA
                     TLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASQSVLK
                     NNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNAPYVQVSAIDYSKNFS
                     GEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVLVNGANLPTTT
                     FPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSKWERITA
                     TPKTGGPSYIYEIRVKSWWVNAGDAFMIYSLAENFCSSNGYTLPLGDHLNHSRSRGIG
                     SLYSEWGDMGHYTTEAGFHSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYATCY
                     KNL"
     gene            complement(4061..5113)
                     /locus_tag="ACHJ48_004232"
     CDS             complement(4061..5113)
                     /locus_tag="ACHJ48_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO4709669.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            5427..6743
                     /gene="shiA"
                     /locus_tag="ACHJ48_004233"
     CDS             5427..6743
                     /gene="shiA"
                     /locus_tag="ACHJ48_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO4709670.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQYNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            6845..8299
                     /gene="amn"
                     /locus_tag="ACHJ48_004234"
     CDS             6845..8299
                     /gene="amn"
                     /locus_tag="ACHJ48_004234"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO4709671.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         2224 a         2008 c         1989 g         2183 t
ORIGIN      
        1 cctcgaaaat aacggtattg ccatcaccca ggccaatggg gaagcgcatg tcacgctcaa
       61 aggtaaaaaa gcgggtacgc atacggttac cgcaacgctg agtaataaca ataccagtga
      121 ttcacagccg gtaacgtttg tggcggacaa aacctcggct ctggttgttc ttcagatatc
      181 aaaaaatgag atcacaggta atggcgtcga tagcgcaacg ctaactgcaa cggtcaaaga
      241 tcagttcgac aatgaggtga acaatcttcc ggtaacattc agcacagctt cttcaggcct
      301 caccctgacc ccaggggaaa gtaataccaa tgagtctggc atcgcgcagg ccactctcgc
      361 aggcgttgcc tttggtgagc agacggtcac tgcatcactg gctaataatg gtgccagcga
      421 caacaaaact gtgcatttta ttggcgacac agcggcggca aaaattatcg agttgacgcc
      481 tgtcccagac agcataatcg caggtacccc gcagaacagc tccggcagcg tcatcaccgc
      541 cacagtcgtt gataataatg gctttccggt gaaaggtgtg actgtgaact tcaccagcaa
      601 cgcagcgaca gccgaaatga cgaatggcgg tcaagccgtg acgaacgaac agggtaaggc
      661 taccgtcact tataccaata cccgctcctc gatagaatca ggagcgagac cggataccgt
      721 tgaggccagt ctggaaaatg gtagctccac gcttagcaca tcaattaatg tcaacgctga
      781 tgcgtctacg gcacatctca ccttgctaca ggcacttttt gatacagtct ccgcaggcga
      841 cactaccaat ctgtatattg aggtgaagga taattacggc aacggagtac cccagcagga
      901 ggtaaccctc agcgtttcac caagtgaagg tgtgaccccc agtaataacg ctatatatac
      961 gaccaatcac gacggcaatt tttacgcaag ctttaccgct acaaaagccg gggtatacca
     1021 agtgacggca accctcgaaa atggcgattc gatgcaacaa acagtgacct atgtgccgaa
     1081 cgtagcgaat gctgaaatct cgctggcagc ctcgaaggat ccggtaattg ccaacaataa
     1141 cgatctcacg acactaacag caacagtcgc tgatacagag ggcaatgcga tagccaacag
     1201 tgaggtaaca tttactctgc cggaagatgt gagggcgaac ttcacgctgg gcgatggcgg
     1261 taaagtggtt actgatactg aaggcaaagc gaaagtcacg ctgaaaggta caaaagcagg
     1321 cgctcatact gttacagcat cgatggctgg cggtaagagt gagcagttgg tggtgaactt
     1381 tattgcggat acactcactg cgcaggttaa tcttaacgtt accgaggaca attttatcgc
     1441 taataacgtc gggatgacca ggctgcaggc aacagtgact gatggaaacg gcaacccgtt
     1501 agccaatgag gcggtgacat tcacgctacc ggcagatgtg agcgcaagct ttactctcgg
     1561 acaaggcggt tccgccatta ctgacatcaa cggcaaggct gaagttacac tgagcggtac
     1621 aaaatccggc acctaccccg tgacagttag cgtgaacaat tatggtgtca gtgatacgaa
     1681 acaggtgact ttgattgccg atgctggtac cgcaaaacta gcctccttaa cctctgtata
     1741 ctcattcgtc gtcagcacga ccgagggcgc gaccatgact gcaagcgtca ctgacgctaa
     1801 cggcaacccg gtagaaggta taaaagttaa tttccgcgga acttccgtca cgctaagcag
     1861 caccagcgtt gaaacggatg atcggggttt cgctgaaatt cttgtgacaa gcaccgaggt
     1921 cggactgaaa acagtttcag cctctctggc agataaacct actgaagtca tctcgcgatt
     1981 actgaatgca aaagcagata ttaattctgc aacgattacc agtctggaga tacctgaagg
     2041 tcaggtcatg gtcgcacaag acgtagcagt taaagctcac gtcaacgacc agtttggcaa
     2101 tccgattctt aatgaatctg taacattcag tgcagaacca ccagagcaca tgaccatcag
     2161 ccaaaatatt gtctctactg atacgcatgg tatagccgag gtcactatga cgcccgaaag
     2221 aaacggttcg tatatggtga aagcatccct ggcgaatgga tcctcttatg agaaggatct
     2281 ggtggtaatc gatcaaaaac tgacactctc ggcgtccagc ccgcttatcg gtgtcaattc
     2341 cccaacaggt gcaactctga cggcaacgct aacttctgca aatggcactc cagtggaggg
     2401 tcaggtcatc aactttagcg taacgccaga aggtgcgacg ttaagtggcg gaaaagtgag
     2461 aaccaactct tcaggtcagg ctccagtcgt tctgaccagc aataaagtcg gtacatatac
     2521 ggtgactgca tcgttccata acggcgtaac aatacagaca cagacaatcg tgaaagtcac
     2581 tggcaactca agcaccgccc atgttgctag ctttatcgct gatccatcga ctatagccgc
     2641 caccaacagt gatttaagta ccttaaaggc aacggttgag gatggcagtg gtaacctgat
     2701 cgaaggtctc actgtgtact tcgccttaaa aagcggctct gccacattaa cgtcattaac
     2761 agcggtgaca gatcaaaacg gaatcgcgac aacaagcgtg agaggagcga taacggggag
     2821 cgtcacggta agcgcagtca cgaccgctgg tggaatgcaa acagtagata taacgctggt
     2881 ggcaggcccg gcagacgcct cgcagtccgt ccttaagaac aatcggtcat cattgaaagg
     2941 agactttacc gatagtgctg agctacatct tgttctgcac gatatatcag gcaatccgat
     3001 caaagtttct gaagggctgg aatttgtgca gtcaggtacc aacgcgccct atgtgcaagt
     3061 tagtgcaatt gactacagta aaaatttctc aggcgagtac aaagccactg ttacaggcgg
     3121 cggagagggt atcgcaacgc tgatccctgt attgaatggt gttcatcaag cgggtctgag
     3181 taccacaata caattcactc gcgcagaaga caaaataatg agcggtacag tgttagtcaa
     3241 tggtgctaac ctaccgacaa ctacattccc ttcgcagggg ttcactgggg cgtattatca
     3301 gttgaataat gacaactttg ccccaggaaa aacggcggct gattatgagt tttcaagctc
     3361 tgcctcctgg gttgatgttg atgctaccgg taaagtgaca tttaaaaatg tcggcagcaa
     3421 atgggagagg attacggcga cgccaaaaac aggcggccct agctatatat acgaaatccg
     3481 agtgaagagt tggtgggtga acgccggcga tgctttcatg atatacagcc ttgctgaaaa
     3541 tttttgcagt agcaatggct acacacttcc ccttggagac catttaaacc atagtcgttc
     3601 ccgaggcatc gggtcactgt acagtgaatg gggagatatg gggcattaca cgactgaagc
     3661 tggttttcat tcaaatatgt attggtcatc gagtcccgca aactcaaacg aacaatacgt
     3721 agtttccctg gcaacaggtg atcaaagcgt atttgaaaag cttgggtttg cttatgcgac
     3781 atgttataaa aacctctgat tttccgttat aatttcttaa agagtgctcc attcggggca
     3841 ctctttttta cattccctct atagagcttg cccttctctc acagaaaatg aataaataaa
     3901 aatgcgatac cgcaagaatc gcgttcgatg tgactatgat cgtatccggt gagtacagct
     3961 tttatccaaa ggctggtttt ctgtgatgat tgaccagcct cagagtattg atgacaccac
     4021 cgttcataac agatcggcat tactatgcat aagtactgtc tcatattctc gccaaaacac
     4081 tttttaatgc ctttgcgaat tcatccagac ttatattgtc aattttttgc ccaacgacga
     4141 ctgactccga aacatcggcc attgcgggcc atcgcgtttt gtaatcagca taaattgcaa
     4201 caacgggggt attataggca cttgccatat gtaatattga ggtgtcagga gttattgcga
     4261 tataagcatc ttttatgatt gctgctgaac gtttaatcga gggtgataag gataaacggt
     4321 agacattatt ataacaatcc actaataccc tggctttatc ttcccctttt ggtccatgaa
     4381 cgatcactat tggtatatct gtttcacttt gaattttttc tattagattt tctgcaatcg
     4441 atagtgaaaa tgtacgatcc tgcgagctac cttctaaatt aagcgcaatg taagatccta
     4501 acgatcgcat ttcctcgcgc acctcatcga gtacatcctc acttagtggc aattcatata
     4561 ttggcctgac tgctggaaaa cccgcctcac gcatcataaa tgcccatgtc atgggtacgg
     4621 gagcccggag tttctgatcc atccgggacg cgttcttaca cacaggggaa tagcacttca
     4681 tggttaaacc aacaacctga aaattcgttt ttgctttcaa ctgactgata aataacatcg
     4741 ttttcaggtt ctttttacgc atcgcctcta tacaaagatc cggcgtaccg tattgctgtg
     4801 ttatgttctt cgctaaatct tttatttctt ttaatgttgc gtgatcctgc atagtcattg
     4861 tgactaatgt taatttagtc tgttcaagtt tgagcgcatt aaagacttct aaattaattg
     4921 tcgacgtaac aattaataga tgcttaattt tatgcaattc aagcgcccga ataacaggaa
     4981 agatggccat agcatcgcca atctgatcgg gaatatggat gacaacaaag tctgtttttt
     5041 caatattgaa attataagct ttataatcgt agtaactaaa tgcaatacgt ctcaacaatg
     5101 atactaaaaa cataactaac ctcgcctccc tactggttat aatgcaatgc agtctatcag
     5161 aatcatcagg gtgcaatttt gtgcatatgc ggacttttat gtttcatatc tcgaacctgt
     5221 ggggcctctg cttaatcctt aaacaacacc agcaactcct gcgctttcat cttccagcga
     5281 atttttcatg ttgccgttaa tcagccataa aatcatttgc agatgcgctc tgtcgaggta
     5341 gtctcataag gttcgtttat agatcgacgc aatgtgagtt accttttcca tactaattat
     5401 aaaaagacag tacaaacagg atcattatgg actccacgct catctccact cgtcccgatg
     5461 aaaggacggt ttcgttaagt cgcgcccgac gagctgcgtt aggcagtttc gctggtgccg
     5521 tcgtcgactg gtatgatttt ttactctatg gcattaccgc cgcactggtg tttaatcgcg
     5581 agtttttccc gcaagtaagc ccggctatgg gaacgctcgc cgcatttgcc acctttggcg
     5641 tcggattcct tttccgtccg ctcggcggtg tcattttcgg tcactttggc gaccgactgg
     5701 gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca gccttgattg
     5761 gtattcttcc ttcattctcg accattgggt ggtgggcacc tattttactg gtgacgctgc
     5821 gtgccattca gggatttgcc gtcggcggcg aatggggagg cgcggcgttg ctttccgttg
     5881 aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt ggctacggtg
     5941 taggtttact gctttcaacc ggactggttt cattgatcag tatgatgacg actgacgaac
     6001 agtttttaag ctggggctgg cgcattcctt tcctgtttag catcgtactg gtactgggag
     6061 cattgtgggt gcgcaatggc atggaagagt ccgcggaatt tgaacaacag caatataatc
     6121 aagcggccgc gaaaaaacgc atcccggtta tcgaagcgct gttacgacat cccggtgctt
     6181 tcctgaagat tattgcgcta cgactgtgcg agttgctgac aatgtacatc gttaccgcct
     6241 ttgcacttaa ttattcaact cagaatatgg ggttaccgcg cgaacttttc cttaatattg
     6301 gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg cttgccgatc
     6361 gttttggtcg ccgcagggtt tatatcacag gcgcgttgat cggaacgttg agcgcatttc
     6421 ctttctttat ggcgcttgaa gcacaatcta ttttctggat agttttcttc tccataatgc
     6481 tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca accgatgttt accgaaatgt
     6541 ttggtgccag ttatcgctat agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
     6601 gcggtggatt tacacctttt attgccgctg cgctcatcac ttactttgcc gggaactggc
     6661 atagcgtcgc catttatttg ctggctggat gtctgatttc cgcaatgacc gctttgttga
     6721 tgaaagacaa tcaacgcgct tgatagtctg gcgaagatca tccgatcttc gccttacact
     6781 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
     6841 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
     6901 gctgtatgag caatctgtag tcgcattacg caacgccatt ggcaactata ttacaagtgg
     6961 cgaattacct gatgaaaacg cccgcaaaca aggtcttttt gtctatccat ctctgaccgt
     7021 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
     7081 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atcttaatga
     7141 acaacttacg ttgctgtatc aggattatgg cgcgcatatc tcggtgcaac cctcacagca
     7201 tgaaatccct tatccttatg tcatcgatgg ctctgaatta acacttgatc gctcaatgag
     7261 cgctgggtta actcgctatt tcccgacaac agaactggcg caaattggcg atgaaactgc
     7321 agacggcatt tatcatccaa ctgaattctc cccgctatcg cattttgatg cgcgccgcgt
     7381 cgatttttcc ctcgcgcggt tgcgccatta taccggtacg ccagttgaac attttcagcc
     7441 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
     7501 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     7561 caccgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     7621 gatgccagca tggcatttaa ttaccgccga tggtcagggt attactctgg tgaatattgg
     7681 cgtgggaccg tcaaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatgt
     7741 ctggttgatg attggtcact gtggcggatt acgtgaaagt caggccattg gcgattatgt
     7801 acttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cacccgatat
     7861 tcctattccg agcattgctg aagtgcaacg tgcgctttat gacgccacca agctggtgag
     7921 cggcaggccc ggtgaggaag tcaaacagcg gctacgtact ggtactgtgg taaccacaga
     7981 tgacaggaac tgggaattac gttactcagc ttctgcactt cgttttaact taagccgggc
     8041 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     8101 atacgggaca ctactgtgtg tttcagataa accgttgcat ggcgagatta aacttcccgg
     8161 tcaggctaac cgtttttatg aaggcgctat ttccgaacac ctacaaattg gcattcgggc
     8221 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     8281 tgagccgccg ttccgataat aagttctgaa ccttcttatc aaaaatagat gtgaaggatt
     8341 aagtaagaca tctggcaaat agcccgcaat aaacgcgggc tgtttgcttc cttgggcgga
     8401 tacg
//
LOCUS       DAZFDC010000165         8383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFDC010000165 DAZFDC010000000
VERSION     DAZFDC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.165"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(229..879)
                     /gene="zinT"
                     /locus_tag="ACHJ48_004235"
     CDS             complement(229..879)
                     /gene="zinT"
                     /locus_tag="ACHJ48_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO4709672.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLAPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1136..1771)
                     /gene="msrQ"
                     /locus_tag="ACHJ48_004236"
     CDS             complement(1136..1771)
                     /gene="msrQ"
                     /locus_tag="ACHJ48_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO4709673.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWIILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKSRSLFNRLRKQVHNKLSV"
     gene            complement(1772..2776)
                     /gene="msrP"
                     /locus_tag="ACHJ48_004237"
     CDS             complement(1772..2776)
                     /gene="msrP"
                     /locus_tag="ACHJ48_004237"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO4709674.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2884..3297)
                     /gene="hiuH"
                     /locus_tag="ACHJ48_004238"
     CDS             complement(2884..3297)
                     /gene="hiuH"
                     /locus_tag="ACHJ48_004238"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO4709675.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3430..4101
                     /gene="hprR"
                     /locus_tag="ACHJ48_004239"
     CDS             3430..4101
                     /gene="hprR"
                     /locus_tag="ACHJ48_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO4709676.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4101..5459
                     /gene="hprS"
                     /locus_tag="ACHJ48_004240"
     CDS             4101..5459
                     /gene="hprS"
                     /locus_tag="ACHJ48_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO4709677.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGAK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(5567..6418)
                     /gene="hchA"
                     /locus_tag="ACHJ48_004241"
     CDS             complement(5567..6418)
                     /gene="hchA"
                     /locus_tag="ACHJ48_004241"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO4709678.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPQKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(7010..8196)
                     /gene="ompC"
                     /locus_tag="ACHJ48_004242"
                     /pseudo
     CDS             complement(7010..8196)
                     /gene="ompC"
                     /locus_tag="ACHJ48_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
BASE COUNT         2309 a         1756 c         1942 g         2376 t
ORIGIN      
        1 ccagccaata taagcaatgg cgtgagcaca ggtatctttc tctgttggcc gtattgtgta
       61 tggaatccgt tattggttaa ctcaatgcgg gttagcttat taatatacag acacgtcata
      121 gtgatgagcg tgtctgtagc tagcttgcgt tcagtggcgt ttgtggatgg accagtggca
      181 gttatgatga catactaaag cggcatcgag gcgttatcat gagaatactc aatgagacat
      241 catttcctcg accacttctt cgctactcaa ctgatatgga taatacgttg gccagttttc
      301 catttcattt aataaagatt gctgtgaatc attacccata aaaatgtgaa aatgagatga
      361 ttttcgtggt gcaattatat gatcgctaaa ttgtatatat ttaggggctt tgctttcagg
      421 atctttacat tcgaataagt aacgaacgcc tttcttgcct gatttatagg tgagtatttt
      481 gtatccatcg taatcatatt tacaggatgt tgtttcatta tttctatgga attcaacaat
      541 gccgtcctca atgccaatca tctcgatatc tgttgcatag cctttgtgat aataatcttt
      601 aatttcagca aatgttttag ttttatctgc atccgctttc ttctgaaaga cgggggcaag
      661 tttgccactt tgcagtaaag gataaacgga ttgccagact ccatcccagt cactgagcgt
      721 tcggttttgt acattggcat catcaaaaac accattagca gctttttgtt cgacctctgt
      781 taagggtttg ccgtgtgagt gatgaccatg cgaaaaggca ggagcgctaa caataaagac
      841 acctaaagca acagccagtt tgtgaagacg aatcgccaaa acagtttcct ccagagtcat
      901 tgtatatgat gtgtaatgtt atattgtaac atatgacaaa atgcaacaac tcttaggaga
      961 tgagagcata gttttctcag gtgtacttat ctatcttatt gaaataaaat gaattatgtt
     1021 gataatttga ggttgatgac aaacgataca taacctgatg tgatctgcgt aggccggata
     1081 gggcgctcgc gccacatccg gcagggataa cgcgtacttt gtcggcaata tgaagttata
     1141 cagataattt gttgtgaacc tgcttgcgta accggttaaa aagggaacgc gacttcttat
     1201 accgtaaggc taaaagcagt acagccagcc cagcgtagat gagcggctgc ggtgaaataa
     1261 tcttcactga ccacagataa tgtattgggg ccaggatcgc gacaagatag acgaaattat
     1321 gcaactgttg ccagtgcttg ccgagttttc gctgcatcgc ctgggttgaa gtaaacgcta
     1381 aagcaagcaa aataatccag ctgataattc ctaacgttaa ataaggccgg gtaattaact
     1441 ccttgcctaa taacgccaga ttattcacgc ccagctccag caatgcgtaa ctggttaaat
     1501 gcagtgtcgc ccaggcaaag caccataagc ctaacaggcg gcgtgtgcgt atcaataacg
     1561 gctgttttgc gtagcgtgcc agaggtgtga ttaacaaggt cgccagcaaa aatttcagtg
     1621 cagtgcgacc ggtaaaatgc tgaatatctt tcaccggatc ggcacccagt ccaccgtgat
     1681 tgatcgccca gaccagccag agaaacggca acaatccggc aagatgcagg caaactttca
     1741 gccatgtcac ctgttttgct gtcagacgca ttcagaaatt ctcccgcaaa tccaggccac
     1801 gatacagcga tgccacctgg tcggcgtaac cattaaacag tagcgttggc tggcgctgta
     1861 catcgaggat gccgcctgaa ccaataaatc gttcggtagc ctgtgaccag cgtgggtgat
     1921 caacatgcgg attaacgttg gcgtaaaaac cgtattcgtc aggcgctgcc agattccagg
     1981 tggttggcgg acgctcgcgg gtcagcttaa tactgacgat cgatttaatc cctttaaagc
     2041 catatttcca cggcacaatc agtcgcaccg gcgcgccatt ttgtggcggt aacgccttgc
     2101 cataaacacc tacggtcatc agtgtgagcg gatgcattgc ttcgtcgaga cgcaatcctt
     2161 cgacataagg atatttcagc ccgccgccga taaagcggtc ctgctggcct ggcatctgtt
     2221 ccggtgcata aattgtttcg aaagcgacat acttcgcatt gctggtgggt tcggcaagcg
     2281 ccagcaattt gtgcagcgga aaaccaatcc acggcaccac catcgaccac gcttccacgc
     2341 agcgcatacg ataaatacgc tcttccagcg ggaagcgacg ggttaaatca tcgtgatcga
     2401 gggtcaatgg ttttgccact tcgccgctga ttttcagtgt ccatggatcg gttttcaggc
     2461 taccagcatt agcggcggga tcggctttat ccagcccgaa ttcatagaag ttgttataac
     2521 cggagacttt atctgctggc gtcagtggca ggttattttg ccaggcggca ggcttgctga
     2581 actccagcgc ttttccggcg ggggcgggtg ggcgatcgtt ccctttaaac cagctaagca
     2641 gatcggcatg cgcagcgtga ggcaaagaaa gtgcagctgc gctgatgccc agtgctttta
     2701 acacctgccg acgcttcata aagaataccg actcggccgt aacatctgat tcttttaaaa
     2761 attgattctt tttcataagc catcacactt tttgtcgtta gccagcaaga gccgtttact
     2821 gttaatctgt cagggagtgt cctccctaac agtagcagag atctgctttt acatatttat
     2881 gcattaactg ccacgatagg ttgaataccc atattggcta agtaataaag gcacatgata
     2941 atgctcgttc actttattaa tatgaaattc aacggggatc tcagggaaga aactttcaag
     3001 attttgtttc ttgaaatagt ccccggtttt aaatacgaca cggtaatcgc ccgtagttgc
     3061 agtttgctcg ggccacagtg ccttaattcg tccatcctta tctgttttgg cggtattaag
     3121 ttgtaaccag ccgttgtccg ctttcttttc aagagtgact gtcacgtcgg cagcgggttt
     3181 tcctgtttgc tggttcaaaa tgtgcacgct aagaatgttt tgttgtgcag catagaccaa
     3241 agaaggtaat gaaaatgcag ccgttactac ggagagtact aaataacgct ttaacatgtt
     3301 tatatccttg tcatgtgaat gagttttgag gatagcgaaa aagcgtccag ggtaacctta
     3361 cagcagcatt acaattttgt aatgaaccag gctgtttcta tgacatatga tttatggcat
     3421 attattttca tgaagatttt acttattgaa gataatcaaa ggacccagga gtgggtaacg
     3481 caggggcttt ccgaagcggg ttatgtcatt gatgccgttt ctgatggcag agatgggctt
     3541 tatcttgcgc tgaaggatga ttatgcattg atcattctgg atattatgct tccgggtatg
     3601 gatggctggc agatcttaca aacgttaaga acggcaaagc aaacccctgt tatttgcctt
     3661 actgcaaggg attctgtcga tgacagagtc agagggctgg acagtggagc aaatgattat
     3721 ctggtaaaac ctttttcatt ttctgagttg ctggcaaggg ttcgggcaca attaaggcaa
     3781 catcacgctt tgaattcaac attagaaatc agcggcttaa gaatggactc tgttagtcaa
     3841 agtgtgagca gggacaatat cagtattaca ctgacgcgca aggagtttca gttactttgg
     3901 ctactggcct ccagagctgg cgaaattata cccagaacgg ttattgcgag tgaaatttgg
     3961 ggaatcaact ttgatagtga taccaatacg gtggatgtcg ccattcgcag gctccgcgca
     4021 aaagttgatg atccttttcc tgaaaagcta attgccacaa tccgtgggat gggatattcg
     4081 ttcgtagcgg taaaaaaata atgaaaagat tatctataac cgtccgttta accttgcttt
     4141 ttatattgct gctgtctgtt gctggcgccg gaattgtctg gactctctat aatggcctcg
     4201 caagtgagtt gaaatggcgc gatgatacaa cactcattaa ccggacagcg cagatcaagc
     4261 agttattaat tgatggggta aatccagata cgttacctgt atactttaac cggatgatgg
     4321 atgttagtca ggatatcttg atcattcatg gtgatggcat caataaaatt gttaaccgga
     4381 caaatgtcag tgatgacatg ttaaataaca tacctgctag tgagacaatc agcgcagctg
     4441 gcatttacag aagcattatt aatgatacag agatagatgc tttacgaatt aatattgatg
     4501 aagtttcgcc atcattaacg gttactgtgg ctaaattggc ttcagccaga cataacatgc
     4561 ttgaacagta taaaatcaat agcattataa tttgcattgt cgccattata ctgtgctcag
     4621 tattaagtcc gctgttaatc cgaacggggt tacgagagat caaaaagttg agtggtgtaa
     4681 cggaagcgct gaattataac gatagccggg agcctgttga ggttagcgca ttaccgagag
     4741 aactaaaacc tcttgggcag gcgttgaata aaatgcatca tgctttagtc aaagattttg
     4801 agcgtctaag tcagtttgct gacgatctcg ctcatgaact tagaacgcca attaatgcat
     4861 tactgggtca gaatcaggtt acgctcagtc aaaccagaag tatcgctgaa tatcaaaaaa
     4921 caattgccgg taacattgaa gagctggaaa atatttcgcg gttaacagag aacatactgt
     4981 ttcttgccag ggcagataaa aacaatgttt tggtgaaact ggactcgctt tctctcaata
     5041 aggaagtcga aaatttgttg gactatcttg aatatctttc agacgagaaa gagatttgct
     5101 ttaaggtcga gtgcaatcag caaatctttg cggataaaat tttactgcaa cgaatgttat
     5161 cgaatcttat tgttaacgct attcgatatt cgccagaaaa atcgcgtatt catataacca
     5221 gttttcttga taccaacagc tatcttaata ttgatatcgc cagccctgga gcgaaaatta
     5281 atgagcctga aaaactcttc cgtagatttt ggcggggaga taattcgcgt cattccgtag
     5341 gtcagggact tggtctttct ttagtcaaag cgattgccga attgcatggg ggaagtgcta
     5401 cgtatcacta tctcaataag cataatgtgt tccggattac gttgccgcaa agaaattaac
     5461 atagggcttc agtacgccat aaattaaccg tgaaaaaacg cagccacgtc gtgagtacta
     5521 acgcggctgc gattgattat gcgcttacat tcaaacgtaa cagggattaa cccgcgtaag
     5581 ctgccagcat ttcctgcgcc gccagtttac ccaacgcatt cgctgcaaaa ggactgtcgc
     5641 cggtgagaag tttacggtcc ttatgtactc gcccggtgat gtcgtcatta atgatgttca
     5701 tgcccatttt cttcagttct tcgccgaagt accaggtgag atgacccggc atatagccaa
     5761 tctctggcgt ttgtttgtct gcggcgtctg ggaatgcgca aatggaataa ccattcagtg
     5821 gattatcgcc gtggcgaagc gccagaaaag ccgccgggcc gtggcaaagg gagataacaa
     5881 aacggtcatt tttgattgcc cactgtaaag cggcagctac gtcctggctt tcaggtaaac
     5941 caataagtgc gccatgacca ccaggaacaa agattgctgc atattcgcta tcagcgttga
     6001 ggctggcgac aacatccgcg agtttcttcg gattgcggaa caacgattta tgctgctcaa
     6061 agaatggcat tactttttca tctttctgcg gcatagccca gtattcaaac ttggtcatca
     6121 gaccggaaat ggtcgccact tcgaattcga aacctgcagc atggagatga tacaacggca
     6181 gcaacgtttc aatcggatgg ttaccggtcg agaacagttt tccgttatcg gtcggcaaat
     6241 aacgttcgtc cgcggcgatc accagaattt tatgtttacc gcgataaggt tttggatagt
     6301 ccacgccatc aagatcagag acaggactgg tatattggct aagcgaatat tctgaaggga
     6361 agaatgcatt atcttcagca atatcgacct gcggattttt actggtttga acagtcatag
     6421 tgtattcctt attgttgctt agttagggta gtcactatat ttgcgactga gctggtcgct
     6481 atttttaacc aggctggaaa attgagtcaa tatatcgaaa gcggtagtcg gcgggtgtaa
     6541 gtatgtaatg atgatgctgc actggtgttg aatattattc acgccaaatt ttccttattg
     6601 agcaacttcg tcgcaagtgg cctcaatgat tttagaccat tatcagaggt acggggcggc
     6661 ggggagagat ttagtgctgt ggaatattgt ggtgctgatg aatatttata tcgcggattg
     6721 gtagagaaaa agcttatttc atgcactatt tttgcaaata tttctatatg ggaagcgtgt
     6781 ttgttacgga ttaattcata aagaaatgca tttgggtgtt ggcttgaaga gtcgctggtt
     6841 gatgttattg tgccaggagg ggcagggtaa gggggtgtat cgataagctt tggcaattgc
     6901 gtgtgccgca gatggagatg aaatgccaat aaaaaagcct gagttcgatg agggacgaac
     6961 tcaggctagt gttcagtttt acaataacac gccgcggcgc gttggctgat tagaactggt
     7021 agactaagcc cacagccacg atatcatcgg tagcgatgcc gttttctttg tagaagtcgt
     7081 cgtcgttgtc cagcaggttg attttgtaat caacataagt ggacatgttt ttgttgaagt
     7141 agtaggtcat accaatatca acatatttaa ccagatcttt gttggtgtaa tgatgattac
     7201 cctggctgtc gaagttatca ctatttacac cgcctaaatc cttaccttta gattgcagat
     7261 aagcgatgga cgggcgcaga ccgaagtcga actgatactg agcaacaact tcaaagtttt
     7321 gggttttgtt agcgatggca tcagcatcac cgtaaccagt catgttacgg ggttcagcat
     7381 acatagctgc taagtaaacg ttattagcgt cgtatttagc accaacggtc catgcttctg
     7441 ctgtttcacc gccagcagtt gcatcgaagc gatctccata acgagtgtta tgagtacctt
     7501 tgtgaacctg attgtcggta cggtcagagt tagagtaagc agcgcccaga cttaaaccaa
     7561 agtcaaagtc ataggaagtg gacatactga accgtcgccg ttctctttag caagtttacg
     7621 tccattaccg ttacctgaac cttcttgatc aaataatccg cttttctcgt tgttaccctg
     7681 atactggagt gcaaagttca gaccatcaac ttgaccaaag aaaccattgt tacgataggt
     7741 tgctacgctg ttagcacgac cgttcatgaa gttgtcagcg ccagcatagg tatctccacc
     7801 aaattctggc agcatatcgg tccatgcttc tacgtcatat acgacaccgt agttacgacc
     7861 atagtcgaaa gtaccattct gaccaaagcc gaaacctgca aatgccagac gcgttgcggt
     7921 attacccttg tcgctttcag gaccgtttgc gccaatgtta aattcccact gaccataacc
     7981 agtgaattgg tcagcgatct gagtttcacc tttgaaaccg atacgggcat atgacatgtc
     8041 gccatcgctg ctagcatcat cagagaagta gtgcaggccc gctacttttc cgtacaaatc
     8101 cagtttattg ccatctttat tataaatttc agccgcattt gctgcgccag caactaataa
     8161 cgccgggacc agcattgcca gaacttttct tttcattatg tattcccttg tgattataat
     8221 cttcatgaat atatcaataa gtgccgttat ccaaaaaaaa gcacatttgg atactattct
     8281 atgaagttca ttttatttta aagattacat gcaacaaata tatttaaaag atttcaaaat
     8341 gtttccaatc tatttaatat aattcagaat tatgaattct aaa
//
LOCUS       DAZFDC010000166         8290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFDC010000166 DAZFDC010000000
VERSION     DAZFDC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.166"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(8..214)
                     /locus_tag="ACHJ48_004243"
     CDS             complement(8..214)
                     /locus_tag="ACHJ48_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO4709679.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            527..616
                     /gene="kdpF"
                     /locus_tag="ACHJ48_004244"
     CDS             527..616
                     /gene="kdpF"
                     /locus_tag="ACHJ48_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO4709680.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALIHAEAF"
     gene            616..2289
                     /gene="kdpA"
                     /locus_tag="ACHJ48_004245"
     CDS             616..2289
                     /gene="kdpA"
                     /locus_tag="ACHJ48_004245"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO4709681.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLAWLINDIPLPGTTGVER
                     VLFSALGVSNHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2312..4360
                     /gene="kdpB"
                     /locus_tag="ACHJ48_004246"
     CDS             2312..4360
                     /gene="kdpB"
                     /locus_tag="ACHJ48_004246"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO4709682.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVVATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4369..4941
                     /gene="kdpC"
                     /locus_tag="ACHJ48_004247"
     CDS             4369..4941
                     /gene="kdpC"
                     /locus_tag="ACHJ48_004247"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO4709683.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTDNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQLLVKYIGQPVVNIVELNLALDKLDE"
     gene            4934..7618
                     /gene="kdpD"
                     /locus_tag="ACHJ48_004248"
     CDS             4934..7618
                     /gene="kdpD"
                     /locus_tag="ACHJ48_004248"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO4709684.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RKLALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7615..>8290
                     /gene="kdpE"
                     /locus_tag="ACHJ48_004249"
     CDS             7615..>8290
                     /gene="kdpE"
                     /locus_tag="ACHJ48_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO4709685.1"
                     /translation="MTNVLIVEDEQAIRRFLRSALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPSHFITETG
                     IGYRFML"
BASE COUNT         1727 a         2163 c         2426 g         1974 t
ORIGIN      
        1 tcagcaatca gtaaaaatca ccggttgcct tttcggcctg atccatccaa accggtttat
       61 cgctggtttt cgaccagacg cgatgcaggt agctgtaaaa gcgggcgcgg tctttccaga
      121 aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc ataagtacgg cgtaaaaaga
      181 taagccatgc aggatattct ctgtagagtt ccatactttt ctcccccttt tgtatctacc
      241 cggtgaatag caccggaaaa atgaatttgt ttatctgatg aaaatagtac cgccttttgt
      301 gtaattttac tactcatccg accacttatt tttgcttatt gatggtttat ttacattcac
      361 cctgtaatta agttacacaa aagttaaatt aatactaaac attagttaaa tcatggcttt
      421 tgccattttt atactttttt tacaccccgt ccgcagattt ttgcgaaatc tttgcagcca
      481 gaattctacc cttccggtat cacttttagg ccactggagg tgcactgtga gtgcaggcgt
      541 gatagccggc gtgttgctgg tgtttttatt actgggttat ttggtttatg ccctgatcca
      601 tgcggaggca ttctgatggc tgcgcaaggg ttcttactga ttgccacgtt tttactggta
      661 ttaatggtgc tggcgcgtcc cttaggtagc gggctagcgt ggctgattaa tgatattcct
      721 cttcctggta cgacgggcgt tgaacgcgta ctctttagcg cgcttggcgt ctctaaccat
      781 gagatgaact ggaagcagta tctctgtgcc attctcggcc tgaacatgct ggggctggcg
      841 gtgctgtttt ttatgttgct cggtcagcac tatctgccgc ttaatccaca gcagttgccg
      901 gggctgtcgt gggatctggc gctgaatacc gccgtcagct ttgtcacgaa taccaactgg
      961 caatcttata gcggtgaaac cacgttgagc tatttcagcc agatggcggg cttaacggtg
     1021 caaaactttc tttctgccgc cagcgggatt gcggtgattt ttgccctcat tcgtgcattt
     1081 acccgccaga gcatgagcac actcgggaat gcctgggtcg atctgctacg catcacgtta
     1141 tgggtgctcg tccctgtggc gttggtgatc gcactgtttt ttattcaaca aggtgcgctg
     1201 caaaactttc tgccctatca ggcagtgaat accgttgaag gagcgcaaca gctgttacca
     1261 atgggtccgg tggcttctca ggaagcgatc aagatgctcg gcactaacgg cggtggtttc
     1321 tttaatgcca actcgtcgca tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag
     1381 atgctggcga tcttcttgat cccaacagcg ctgtgctttg cctttggtga agtgacgggc
     1441 gatcgccgtc aggggcgcat gttgctgtgg gcgatgtcag tgatttttgt catctgcgta
     1501 ggcgtggtga tgtgggcaga agttcagggt aatcctcatc tgctggcgct cggcgcagac
     1561 agcagcatca atatggaagg taaagagagc cgtttcggcg tgctggtcag tagcctgttt
     1621 gcggtcgtga ctacggcggc gtcctgtggc gcggtgattg caatgcatga ttcgtttacc
     1681 gctctcggtg gcatggtgcc gatgtggctg atgcaaattg gtgaagtggt attcggcggt
     1741 gtcggttctg gtctttacgg catgatgctg tttgtcctgc tggcggtgtt tattgccggg
     1801 ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa tcgacgtacg tgagatgaaa
     1861 ctgactgcac tggcgattct ggtcaccccg acgctggtgc tgatgggcgc ggcgctggca
     1921 atgatgaccg acgccggacg tagcgccatg ctcaaccctg gcccgcatgg ttttagcgaa
     1981 gtgctgtacg ccgtgtcatc cgccgctaac aacaacggca gcgcctttgc cggattaagc
     2041 gccaactctc cgttctggaa ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg
     2101 gtgattatcc cggtgatggc aattgccggt tcgctggtga gtaaaaagag ccaacccgcc
     2161 agctccggca cgctgccaac gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg
     2221 ttgctggttg gcgcactgac ctttatccct gccctggcgc ttggtccggt ggcggaatat
     2281 ctctcctgat gatattgagt gagcactgaa tatgagtcgt aaacaactgg cgctattcga
     2341 accaacactt gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca
     2401 atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca cctgtattag
     2461 catcgcgatg gcaagcggcg cgatgcctgg caatgcgctg tttagcgtgg ccattagcgg
     2521 ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag gcgctggcag aaggccgcag
     2581 taaagcgcag gccaacagcc tgaaaggggt gaaaaaaacg gcctttgccc gcaagctgcg
     2641 tgagccgaaa tatggcgctg cggcggacaa agttcccgcc gatcaactgc gtaaaggcga
     2701 tatcgtactg gtagaagcag gcgatattat cccctgcgat ggtgaagtta ttgaaggcgg
     2761 cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg
     2821 cggcgatttt gcctccgtca ccggcggcac gcgtattctt tctgactggc tggtgattga
     2881 gtgtagcgtt aaccccggcg agacatttct ggatcggatg atcgcgatgg tggaaggcgc
     2941 acagcgacgc aaaacgccga acgagattgc cctgaccatc ctgctgattg ccctgactat
     3001 cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc gcgtggggcg gtaatgcagt
     3061 cagcgtaacg gtactggtgg cgctgctggt ctgtctgatc ccaaccacta ttggcggcct
     3121 gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg ggcgcgaatg tggttgccac
     3181 cagcggacgt gcagttgaag cggcaggtga cgttgacgtt ctgctgctgg ataaaaccgg
     3241 caccatcaca ctcggtaacc gtcaggcatc ggagtttatc cctgcgcagg gcgtggatga
     3301 aaaaacgctg gctgacgccg cacaactggc ttcgctggct gatgaaacgc cggaaggccg
     3361 cagtattgtg atcctcgcca agcagcgttt taacctgcgc gagcgcgatg tgcagtcgct
     3421 ccatgccacc tttgtaccgt ttactgcgca aagccggatg agcgggatca atatcgacaa
     3481 ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc catgttgagg ctaacggtgg
     3541 tcacttccct gccgatgttg atcaaaaagt cgatcaggtt gcgcgtcagg gggccacacc
     3601 gctggtagta gtagaaggtt ctcgtgtgct gggcgttatt gcgttgaaag atatcgtcaa
     3661 aggcggcatt aaagaacgct tcgcccaact gcgcaaaatg ggtattaaaa cggtgatgat
     3721 taccggcgat aaccgtctga ctgccgccgc gatagctgcg gaagcgggcg tcgatgattt
     3781 tctcgccgaa gcgacaccgg aggccaagct ggcattgatc cgtcagtatc aggcggaagg
     3841 ccgtttggta gcaatgaccg gcgacggcac caacgatgct ccggcgctgg cgcaggcaga
     3901 tgtcgcggtg gcgatgaact ccggtactca ggcggcgaaa gaagcgggca atatggtgga
     3961 tctcgactct aacccgacca agttgatcga ggtggtacat attggcaaac agatgctgat
     4021 gacccgtggc tcgctgacca ccttcagcat tgccaacgat gtggcgaaat acttcgccat
     4081 tattccggcg gcattcgcgg taacgtatcc acagttaaat gcgctgaaca tcatgcgcct
     4141 gcattcgccc gactccgcca ttctcagtgc ggtcattttc aacgccttga ttatcgtctt
     4201 tttgattccc ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt
     4261 gcgccgtaac ttatggattt acggtctggg tgggctgctg gtgccgttta ttggtatcaa
     4321 agtcattgat ttactactga ccgtttgcgg tctggtgtga ggtttaccat gcgtggatta
     4381 cgtccggcat tatcgacatt tatctttctg ttattgatta ctggcggtgt ttacccgctg
     4441 ctgaccaccg cgctggggca atggtggttt ccctggcagg ccaacggttc gttgatccgt
     4501 gaaggtgata cggtgcgggg ttcggcatta atcgggcaga attttaccga caacggctat
     4561 tttcatggtc gcccgtcggc aacggcagaa atgccttata atccacaggc ttctggcggg
     4621 agcaatctgg cggtcagtaa ccctgagctg gataaactaa tagccgcacg cgttgctgca
     4681 ttacgggccg ctaacccgaa tgccagcacg agcgttccgg ttgaactggt gacggcgtcg
     4741 gcaagcgggc tggacaataa catcaccccg caagcggcgg cctggcagat cccacgcgta
     4801 gcgaaagcgc gtaatctcag cgttgaacag ctcacgcaac tgatcgcaaa atacagccaa
     4861 caactactgg tgaaatatat cggccagccg gtcgtcaaca ttgtcgaact caatctggca
     4921 ctagataaac ttgatgaata acgaaccctt acgtcccgac cccgatcgtc tgctggaaca
     4981 aactgccgcg ccgcatcggg ggaagctgaa agttttcttc ggtgcctgcg caggcgtcgg
     5041 gaagacctgg gcgatgctgg cagaagccca gcgactgcgg gcgcaagggc tggatattgt
     5101 ggttggcgtg gtagaaaccc acgggcggaa agataccgcc gccatgctgg aagggctggc
     5161 tgttctgccg ccaaaacgcc tggcgtaccg tgggcggcac atcagcgagt ttgatctcga
     5221 tgccgccctc gcccgccgtc cggcgctgat cttaatggac gaactggcgc acagtaatgc
     5281 gccaggttct cgtcatccta aacgctggca ggatatcgaa gaactgctgg aagctggcat
     5341 tgatgttttc acaaccgtca acgttcagca tctggaaagt ctgaatgatg tggtcagcgg
     5401 cgtcaccgga attcaggtac gggaaaccgt gcccgatcct tttttcgatg ccgccgacga
     5461 cgtggtgctg gtggacttgc ccccggacga tctgcgccag cggctgaaag aaggcaaagt
     5521 ctatattgcc gggcaggcgg agcgcgccat tgaacatttt ttccgcaaag gcaatctgat
     5581 cgccctgcgc aaactggcac tgcgccgtac tgccgatcgc gttgatgagc aaatgcgcgc
     5641 ctggcggggg catcctggcg aagagaaagt ctggcacacg cgcgacgcga tccttttatg
     5701 catcggccat aacaccggca gcgaaaaact ggtccgcgcg gcggcgcggc tggcgtcacg
     5761 gctggggagc gtctggcacg cggtgtatgt tgaaacccct gccctgcatc gcttaccgga
     5821 gaaaaaacgt cgggcaattc tcagcgcctt acgtctggcg caggagctgg gcgcggagac
     5881 ggcaacactt tctgatccag cggaagagaa agcggtagtg cgttatgccc gtgaacataa
     5941 tctcggcaag attattctcg gtcgcccagc ctcgcgccgc tggtggcgtc gggaaacgtt
     6001 tgctgaccga ctggcgcgca tcgcccccga tctcgatcag gtgctggtcg cgcttgatga
     6061 accacccgcc cgcacgatta acaacgcgcc ggatagccgc tcttttaaag acaagtggcg
     6121 ggtacagatt cagggatgcg tggttgccgc cgcgttatgc gccgttatca ccttaattgc
     6181 catgcagtgg ctgatggcgt ttgatgccgc caacctggtg atgctgtatc tacttggcgt
     6241 ggtggtggtg gcgctatttt atggacgctg gccttcggtg gttgctaccg tcattaatgt
     6301 ggtgagtttc gatctctttt ttatcgcccc acgcggcacg ctcgccgtct ctgatgtgca
     6361 atatctgctg accttcgcgg tgatgttaac cgtcgggctg gtgatcggga accttactgc
     6421 gggtgtgcgt tatcaggcgc gggtagcccg ttaccgcgag caacgcacac ggcacttata
     6481 tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag gacatcgccg ccaccagcga
     6541 acaatttatt gcctccacgt ttcatgcccg cagtcaggtg ttgttgcccg atgacaacgg
     6601 taaattgcag ccgttaacac atccgcaagg aatgacgccg tgggacgatg ccatcgcgca
     6661 gtggagtttt gataaaggcc tgcctgcggg tgcgggcacc gacacgttac ccggcgtgcc
     6721 gtaccagatt ttgccgttaa aaagcggtga gaaaacctac gggctggtgg tggtggaacc
     6781 ggggaatctg cgtcagttga tgatcccgga acagcagcgc ctgctggaga cgtttacgct
     6841 gttagtcgcc aatgcgcttg agcggctgac gctgaccgcc agcgaagaac aggcgcggat
     6901 ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggcg gcgctttcgc atgatttacg
     6961 cactccgctt acggtgctgt ttggtcaggc agaaatctta acgctcgatc tggcaagcga
     7021 aggatcaccc cacgcccgcc aggccagcga gattcgtcag catgtgctga acactacccg
     7081 actggtgaat aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa
     7141 agagtggtta acgctggaag aagtagtcgg cagcgcgctg caaatgctgg aaccgggttt
     7201 atcgtcgccc atcaatcttt ctctgccaga accgctgacc ttaatccacg ttgacgggcc
     7261 actctttgaa cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca
     7321 ggccgaaatt ggtatcgatg cccacgttga gggcgaaaat ctacaactgg atgtctggga
     7381 taacggcccc ggtcttccgc caggccagga gcagacgata tttgataagt ttgctcgcgg
     7441 gaataaagag tcggcagtac cgggggtagg gcttggactg gcaatttgtc gggcgatagt
     7501 ggatgtacac gggggcacta ttaccgcgtt caaccggccg gaaggtggtg cctgttttcg
     7561 tgttacactt ccccagcaaa ctgcccctga acttgaagaa tttcatgagg atatgtgaca
     7621 aacgttctga ttgttgaaga tgaacaggct attcgtcgct ttctgcgctc ggcgctggag
     7681 ggcgacggga tgcgcgtcta tgaggccgaa acgctgcaac gcggcttgct ggaagcggct
     7741 acccgtaagc cagatttgat tattctcgat ctcggcctgc ccgatggtga tgggattgag
     7801 tttatccgcg acctgcgcca gtggagcgcg gtgccggtga ttgtgctttc cgcacgcagc
     7861 gaagagagcg acaaaatcgc cgcgctggat gccggagcgg atgattatct aagtaagccg
     7921 tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca ctccgccacc
     7981 gccgcgcccg atccgctggt gaaattttcc gatgttaccg tcgatttagc cgcccgcgtg
     8041 attcaccggg gtgaggaaga ggtgcatctc accccgattg agtttcgcct gctggcagtg
     8101 ctgctcaaca acgccggaaa agtgctcacc cagcgccaac tccttaacca ggtgtggggg
     8161 ccaaacgcgg tcgaacacag tcattatttg cgtatttata tgggacatct gcgacaaaaa
     8221 ctggaacagg atcccgctcg ccccagccat ttcattactg aaaccggtat tggttatcgg
     8281 tttatgcttt
//
LOCUS       DAZFDC010000167         8226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFDC010000167 DAZFDC010000000
VERSION     DAZFDC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.167"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            42..1808
                     /gene="adeD"
                     /locus_tag="ACHJ48_004250"
     CDS             42..1808
                     /gene="adeD"
                     /locus_tag="ACHJ48_004250"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO4709686.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDAHGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESAKLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFERNDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1854..3245)
                     /gene="uhpT"
                     /locus_tag="ACHJ48_004251"
     CDS             complement(1854..3245)
                     /gene="uhpT"
                     /locus_tag="ACHJ48_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO4709687.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3383..4702)
                     /gene="uhpC"
                     /locus_tag="ACHJ48_004252"
     CDS             complement(3383..4702)
                     /gene="uhpC"
                     /locus_tag="ACHJ48_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO4709688.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4712..6214)
                     /gene="uhpB"
                     /locus_tag="ACHJ48_004253"
     CDS             complement(4712..6214)
                     /gene="uhpB"
                     /locus_tag="ACHJ48_004253"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO4709689.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIEESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWLQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6214..6804)
                     /gene="uhpA"
                     /locus_tag="ACHJ48_004254"
     CDS             complement(6214..6804)
                     /gene="uhpA"
                     /locus_tag="ACHJ48_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO4709690.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6966..8069)
                     /locus_tag="ACHJ48_004255"
     CDS             complement(6966..8069)
                     /locus_tag="ACHJ48_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709691.1"
                     /translation="MNSYIKIDQTGSNKFKITDLYREFNGETKCYDKKCRDMDFSGRN
                     VKEFIRLLSDHDQFEKDQISELTVAANALKLEVAKNNYNMKYSFDTQTERRMIELIRE
                     QKDLIPEKYLHQSGIKKLKLHEDEFSSLLVDAERQVLEGSSFVLCCGEKINSTISELL
                     SKKITDLTHPTESFTLSEYFSYDVYEEIFKKVVFTPGECDLVRDSLVQDGIKPEKIAK
                     IISCLSSRTGIRNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGRNY
                     HQHVDFSEYGFTQTLCVLGAVVNNANDTFDVANYIYTTKGENIIHNDAEDDSPYVHSD
                     ESYRHKDDYWARDNDIRNLRRNFSGNITIATYK"
BASE COUNT         2168 a         2234 c         2006 g         1818 t
ORIGIN      
        1 ataaataaca atcaaattaa taaaatcaaa atgagaaaaa tatgaataat tccattaacc
       61 ataaatttca tcacattagc cgggcggaat accaggaatt gttagccgtt tcccgtggcg
      121 acgctgttgc cgattatatt attgataatg tctctattct cgacctgatt aatggcggag
      181 aaatttccgg cccaattgtg attaaagggc gttacattgc cggtgttggc gcagaataca
      241 ctgatgctcc ggctttgcag cggattgatg cccacggcgc aacggcggtg ccagggttta
      301 ttgatgctca cctgcatatt gaatccagca tgatgacgcc ggtcaccttt gaaaccgcca
      361 ccctgcctcg cggcctgaca actgttattt gcgatcctca tgaaatcgtc aacgtgatgg
      421 gcgaagccgg tttcgcctgg tttgcccgct gtgccgaaca ggcaaggcaa aaccagtact
      481 tacaggtcag ctcttgcgta cccgctctgg aaggctgcga tgttaacggt gccagtttta
      541 cccttgaaca gatgctcgcc tggcgggacc atccgcaggt taccggcctt gcagaaatga
      601 tggactaccc tggcgtaatt agcgggcaga atgcgctgct cgataaactg gatgcatttc
      661 gccacctgac gctggacggt cactgcccgg gtttgggtgg taaagaactt aacgcctata
      721 ttgctgcggg tattgaaaac tgccacgaaa gttatcagct ggaagaagga cgccggaaat
      781 tacaactcgg catgtcgttg atgatccgcg aagggtccgc tgcccgcaat ctcaacgcac
      841 tggcaccgtt gatcaacgaa tttaacagcc cgcaatgcat gctctgtact gatgaccgta
      901 acccgtggga gatcgcccat gaaggacaca tcgatgcctt aattcgccgc ctgatcgaac
      961 aacacaatgt gccgctgcat gtggcatatc gcgtcgccag ctggtcgacg gcgcgccact
     1021 ttggtctgaa tcacctcggc ttactggcac ctggtaagca ggctgatatc gtcctgttga
     1081 gcgatgcgcg taaggtcacg gtgcagcagg tactggtgaa aggcgaaccg attgacgcgc
     1141 aaaccttaca ggcggaagag tcggcgaaac tggcacaatc cgctccgcca tatggcaaca
     1201 ccattgcccg ccagccagtt tccgccagcg actttgccct gcaatttacg cccggaaaac
     1261 gctatcgggt cattgacgtc atccataacg aattgattac gcactcccac tccagcgtct
     1321 acagcgaaaa tggttttgag cgcaatgatg tgtgctttat tgccgtactt gagcgttacg
     1381 ggcaacgtct ggctccggct tgtggtttgc tcggcggctt tggtctgaat gaaggcgcgc
     1441 tggctgcgac ggtcagccat gacagccata atattgtggt gatcggtcgc agtgccgaag
     1501 agatggcgct agcggtcaat caggtgattc aggatggcgg cgggttgtgc gtggtacgta
     1561 acggccaggt ccaaagccat ctgccgttac ccattgccgg gctgatgagc accgacacgg
     1621 cgcagtcgct ggcggagcaa attgacgcct tgaaagccgc cgcccgtgaa tgcggtccgt
     1681 tacccgatga gccgtttatt cagatggcgt ttctttccct gccagtgatc cccgcgctga
     1741 aactaaccag ccaggggctg tttgatggcg agaagtttgc cttcaccacg ctggaagtca
     1801 ctgaataata aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc
     1861 actgtcaact gctgaatttt tttctcgcgg cggattttgc gttcttccat caccgccact
     1921 atcgccatca ggcagataca accaatcgcg gcgatatcca gcgcagcgaa ggtgcctgcc
     1981 cagccggtaa ggccgaatac tggcgtccca tcggcaatca ttcccagacc taacttggca
     2041 aagctgtcac caatcaggta agcgaaggtg cctttaatac catcggcagc gccaatcgct
     2101 tttttaggta caaagccaac agcagccaca ccaatcaaca attgcgggcc aaagaccagg
     2161 aaacccaacg caaagagaga agccagatag atatattcgt tgctggcatg ttggtacaca
     2221 ccgagcgtgg cgataatcag cgccagcgcg atgcaggcca ccaggccacg gcgaccgttc
     2281 gctaggtcag agagccagcc ccacagcagc gtaccgacca gcgcaccagc ttcaaacagc
     2341 gtaaagccct gaatcgccac cgctttagag agtttcagtt cctggaacgc gtacacagtt
     2401 gaccactggt cgataccaat acgcaccaca tagaggaaaa tgttggcgaa gcacagcagc
     2461 cagatcactt tgtttttcag cacatactca acaaagatct gccacttggt catatcggta
     2521 gattctgtct ctttgtcctc ttcgctgatc tcctcgccga acagctcttc agctttgccg
     2581 aggccataag attccgggga gtcgctgccg taacgcaggc cgataaaacc gacaatcagc
     2641 gcgataatcg acgggaagat aaacatgccg atgacatggc catcgaacag gtaatttgcg
     2701 ccgaacagtg ccacaccagc tgcgcctgca ccgccaaggt tgtgagaaat gttccagaaa
     2761 ccgaggaacg tcccgcgttt acgacgcggc gtccatttgg tgatggtgga gtaactgcac
     2821 gaacccccgg tactctggaa aaagccactt aaggcgtaga aggcaatcat caggaacagg
     2881 ctaaccgagc cgctgcccat actggcgcta aagcccagca tacaaatggc agacaggatc
     2941 agcatgaacg gcaggaattg tttggtgttt ttgccgtcgg cgtagtagga aaccagcgtt
     3001 ttacccacgc cataagtgat ggagaaaccc aggccgatca tccccagctg cgtcatgctc
     3061 aacccgtagg tcgaaatcat atcgttctgc gcgatgttaa agttcttgcg aatcaggtac
     3121 atcgtcaggt agccgataaa gaccaccagg taggattgca tgaacggttt gaaccacatt
     3181 ttgcgccgca cttcgagcgg aaggtccagg gtcggcttgc ggacctggtt taagaaagcc
     3241 agcatgggtt actcctgaaa tgaatacctg cgtgaggcat gcattgtaaa aatcagcagc
     3301 aaatcaggcc tgagatagcg tccaggcaaa acctggaaaa tttcttagtt ttgccggata
     3361 tgaagtgaaa aggtgagatg catcacgctt cgcgcggtgt ctgggcgttc aaaaagggca
     3421 gtaacagcag tgcggaaatc ccggcggcaa tggcgataac cacaaaaaat ccgctccagt
     3481 gccaggtatc gagcactttc gccagcggcc aaccagcaag cgacgccccc agataagcaa
     3541 acaagccgac aaaccccgtc gccgcccctg ccgcctcttt gtgggaacat tctgccgccg
     3601 ccatgccgat taacatctgc gggccaaaga caaaaaaacc aatggtgaag aagcaggttg
     3661 cctgcatcac gtagctggca aatggcatca gccacagcga gccgactgaa agcaaaattc
     3721 cggcagcgaa aatcaaattc atcggcccgc ggttgccgtt aaacagtttg tccgagcccc
     3781 aacccgctac cagcgcaccg ataaatccgc ccagttcaaa catcgtcact gccgtattcg
     3841 ccgtgaccag atcgacgccc agcgtctcgg acatatacaa attgccccag tcgttgatcg
     3901 ccgcccgaac cacatagacc agcacatagc aaaacgaaag cagccagata tacggattca
     3961 acaacacata cttggtgagg atctctttac gcgtcagccc tgccccttct tgttgttgag
     4021 caatctccag cgcgtcgtgt cgccattcac cgaccgctgg taaacctaac gcctgcgggc
     4081 gatcgcgcag ccgccagcag agaaaaatcc ccacgacgat cgccatacaa ccagcaatca
     4141 tcatcccggc acgccagccg taatgcagcg cagctgctgc catcacaatg ggaatgagcg
     4201 caccgccaac gttatgcgcc gtgttccata atgcccacca accaccgcgt tcggtacgag
     4261 aataccaggc cgttaacaga cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt
     4321 tcagcaccca gagcacggca aacgcccata gcgacgtcga gaagccaaac agaatgttga
     4381 tgatgcccgt ggcgataagc cctatcccca taaaataacg ggcatttgag cggtcgctga
     4441 caatgccgga gacaaacttc gacacgccgt aggtaatgta aaacagggtc gctaacaggc
     4501 cgatatcgct acggctgagc acgccattag caaggatttc tggtacggcg gcgttaaaac
     4561 ttttacgcgt gaagtaaaac agcgcgtaac ccagccagat ggtcagcaga atatgccgac
     4621 gccagtagcg ataacgggca tcaatttcat gtttatcagt cattaatggc gcatcggcag
     4681 gcgctttcag aaacggcaac atcatcaaac cttagacata gcgttgaggt agagaaacgc
     4741 tgacacgcgt gccgtgcaga caggaaatgg tcaaggtgcc gcccagcgcc gttacgcgct
     4801 cgcgcattcc ggtgaggcca aaaccttgtt gcccggaacc cggcggtaaa ccgctgccat
     4861 cgtcttcaat caccagcatc aaccgttcat cctgcagcca gccttgcagg gtgaccgcgc
     4921 tggcatcagc atgtttcaca atgttattca gcccttcctg gcagacacga aacagcgtca
     4981 cgcgctggtt ttcgcttaac gctgactctt cgattcgcca ttcgagatgg ctgacaatgc
     5041 cgcgcccttc cagctccatt tcccgcatca gtgagcggat ggcctgctct agggtgagat
     5101 catccaactg gcgcggacgt aaccgcccta acaaacggcg caccgcgtcg tacacgccca
     5161 gcgacagttg ttcgatgagc tgcccgctct gcttcacact ggcgttatct gccgccagtc
     5221 gctggacaat gcccgcttga gtacgaatgg cagtgatggt ctggccgata tcatcatgca
     5281 gctcacgcgc cacatcacgg cgcacgctct cttcagtttc taacaaacgt tcagcgagat
     5341 gctgattgcg cgccagttcc ttttgcagcg actggttaag ttcacgcaac cgctggatgc
     5401 cagcgcccag taacaaccct gtcagacttt gcaccagcag cgagagcaat aaatccacag
     5461 gatgatcgcg ccaggtttga ctggcgatca gcgcgatggc gttcatcaat gtcgctatca
     5521 gcgccccttg ccagccgtag tgccaggcca gcgcgataat cggcagcgcc aggcagaatg
     5581 gcgtaaagcg cgacagttcg tccggcaagc ccaactggag ccagagactg ataacaaaca
     5641 gcagcaagta ccagaccaga tgccgcccgc gccagttgat cggttgagaa actaacgacg
     5701 gcccgagcgg cagccaggtg ttattggcga gataatgcca gaacaccaga catatcgggg
     5761 ccagcgtcag gccgccagtt aaagttaaca gcagcgcatt ccacgactct ttgccgtgcc
     5821 aaagccaggg cagcgactgc aacaacgccg ccgccgttaa cgccgcaccc tgtaacagca
     5881 aggtgcgcca gtcacgttga tggcgatagc gcgagatcag cgccacgggc agtaacgtca
     5941 gtaaactacc gatcatcaat aaggagaaat gggttaaacc gaccgcctgc gttagccagt
     6001 aaatcagcag ccactccgct cccagcagca ccggccagta tccgcgcggg cattgcagca
     6061 ttagccctag ccgcagacca aacggaaata acagcaccgc catatcaggg cgctcaacca
     6121 ggtgcaggct gatactccac aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa
     6181 taacggtaat taagcgggag aacaacgtct tcatcaccag ccatcaaaca tacggcgcgc
     6241 cagttcaacg tcgttactga cgcccagttt ttccatcaga ttggcgcgat ggacgtgtac
     6301 cgttttcggt gacaagccca gttcggcggc aatctctttc accgccattc cttgcgccag
     6361 tttttccgcc acctgccgtt cacgtttggt tagtgggtcc tggcgaccgg atgccagttt
     6421 aatggcaata tccggcgtca gataacagcc gcctgtggca accgtatgca ccgcagcaat
     6481 cagttcgtca gggctacaac gcttggagag aaagccacgc gcccccgcgt taagcgcctg
     6541 ctcaaccagc gccggactgt catgaacgga gagcataatt gtcgccatac ctttcggcag
     6601 ctggcttagc agctccagac cggagatatc gggcatggag atatcgcaaa tacacacctg
     6661 cacaccgcgc cccggcagcc ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac
     6721 ttgcaaatca ggttccagcc ccagcaactg cgcaaagccg gagcggacga tgaggtgatc
     6781 gtctataagg gcaacggtga tcatgattgg gtcctggcgg ataaaaaacg cgcatacctt
     6841 aacgataagc gcgatgttgt tcaagttgga tatggggtat gtattggctt aatgatgttg
     6901 agcttatcgt caaaacgctt acctgagagg taagcgtttg gtgcgtgtta ttgagaaaaa
     6961 gcgaattatt tgtaggttgc aatcgtaata ttgcctgaga aatttctcct tagattgcgg
     7021 atatcattat cccttgccca ataatcatct ttgtgtctat aactctcatc ggaatgtaca
     7081 tagggagaat catcttcagc gtcgttatgg ataatatttt cgcctttagt ggtatagata
     7141 taattagcaa catcaaaggt gtcattcgcg ttatttacga ctgcgcctaa gacgcaaagg
     7201 gtttgagtga atccgtattc tgagaaatcg acatgctgat ggtaatttct accattcaaa
     7261 gcaacaaaac tcggacaaac atctattgta tcgccaacag acgtagcggc gcgacgcaca
     7321 tcaagtgcag cgttatcgtt agcaacatga aatgcttgtg aacaggcata aaacaaattt
     7381 ctaatcccgg tacgactgga taaacaagaa ataatttttg ctattttttc tggctttata
     7441 ccatcctgta ctaaagaatc tctcaccaga tcacattcac caggtgtgaa aacaactttc
     7501 ttgaatattt cctcataaac atcataagaa aaatattcag agagagtgaa agattcggtg
     7561 ggatgtgtta aatctgtaat ttttttgcta agtaattcac ttatagtgct gtttattttt
     7621 tcaccgcaac ataaaacaaa tgaacttccc tccagaactt gcctttctgc atctacaagg
     7681 agtgagctaa attcatcctc atgaagctta agttttttta tacctgattg atgtaaatat
     7741 ttttctggaa ttaaatcttt ttgttccctt ataagctcta tcattcggcg ttcggtttgc
     7801 gtatcaaaag agtatttcat gttatagtta tttttagcaa cttccagctt aagagcattt
     7861 gcagcaactg ttaattctga gatttgatct ttttcaaatt gatcgtgatc tgataataac
     7921 cttataaact cttttacatt tcgaccacta aaatccatat ccctacactt tttatcataa
     7981 cactttgttt cgccattgaa ctcacgatac agatcagtga ttttaaattt attacttccg
     8041 gtctgatcaa tttttatata gctattcatg cctgtaacgg caatctatgt cttataattc
     8101 gcattctccg attcaggcgt aacatgaaaa taaggaacca atttatcact aatatcatgc
     8161 cgatagatat gaaatgaatc taaaagatat aataaaaaac ttcgaaaaat gtcacgaaaa
     8221 gtgtca
//
LOCUS       DAZFDC010000168         8125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFDC010000168 DAZFDC010000000
VERSION     DAZFDC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.168"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            75..617
                     /locus_tag="ACHJ48_004256"
     CDS             75..617
                     /locus_tag="ACHJ48_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4709692.1"
                     /translation="MKHSIIAVAVLSSVFMSAGIFAAEVDTGTLTIKGNIAESPCTFV
                     AGGDSVSINMPTVPTTVFDGKAKYSTYDGAVGVTSSMLKITCPKTIDGVKLSLITNEK
                     ILGNDKAISSSNDTVGYYLYLGDSSDVLDISAPFNIESYKTADGQYAIPFKAKYLKLT
                     DSGVASGTVNSSLVMRVAQD"
     gene            705..1379
                     /locus_tag="ACHJ48_004257"
     CDS             705..1379
                     /locus_tag="ACHJ48_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4709693.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMPNKLPSNKES
                     IFYLNVLDIPPNSPEDEGKNALKFAMQNRIKLFYRPAGIAPVNKTTFKKLRVSRSGNN
                     VVIKNDSPNWVTISDVKANNVKVNYETIMIAPQESQNVSEKNNNANGWQLTIIDDHGN
                     YISDKI"
     gene            1395..3875
                     /locus_tag="ACHJ48_004258"
                     /pseudo
     CDS             1395..3875
                     /locus_tag="ACHJ48_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310942.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3886..4920
                     /locus_tag="ACHJ48_004259"
     CDS             3886..4920
                     /locus_tag="ACHJ48_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4709694.1"
                     /translation="MMFRNRILLIFILWANFTWAGCRTTASLNITDGINVGEILANET
                     SFSKSVVFTGISCDTSTDKIVYKNIQSDWVEVGPFGNGEKLKVKIESLGKTSDTIGKS
                     SNAQAVLPYVVKIARGTPDFTGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKF
                     NWCVNYLTSKLAGDTFTLGLNISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANS
                     KEGTITLKCDNLFGDKKQASRNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPP
                     KGTEAAIKISANGDQGAATSLWKTDKPGVSLNSNIINIPVMASYYVYDEKKVKSGALE
                     ATALINVKYD"
     gene            complement(5002..5340)
                     /gene="rcnB"
                     /locus_tag="ACHJ48_004260"
     CDS             complement(5002..5340)
                     /gene="rcnB"
                     /locus_tag="ACHJ48_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO4709695.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHYKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5558..6409)
                     /gene="rcnA"
                     /locus_tag="ACHJ48_004261"
     CDS             complement(5558..6409)
                     /gene="rcnA"
                     /locus_tag="ACHJ48_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO4709696.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHHHHDHEHHQDHDHDHDHDHDHEHHHHHEHGDNEEYQD
                     AHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATL
                     VVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVH
                     GFMGIMR"
     gene            6530..6802
                     /gene="rcnR"
                     /locus_tag="ACHJ48_004262"
     CDS             6530..6802
                     /gene="rcnR"
                     /locus_tag="ACHJ48_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO4709697.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            complement(6912..7226)
                     /locus_tag="ACHJ48_004263"
     CDS             complement(6912..7226)
                     /locus_tag="ACHJ48_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFO4709698.1"
                     /translation="MKKLLVTVIFVAMMPNAFALSHGDSATLKEGTFNCKKLTDFYEM
                     ISYIQDNDQQAMLSLITSNKCRVLDESMTVEIQSVDDKGFVSFITPGGHGGWAVKQFF
                     EN"
     gene            complement(7236..7583)
                     /locus_tag="ACHJ48_004264"
     CDS             complement(7236..7583)
                     /locus_tag="ACHJ48_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709699.1"
                     /translation="MKSKIFAALLLLPLSTYASSYKCDALQQGQIGSNGKTVTAQSSI
                     DIDGKQMHFSLNGVSWDLSYIGEKQIAKMYSTESGNVSVSFIDANPIIFVLHLVNDDG
                     RKGTYNISECSEL"
BASE COUNT         2366 a         1545 c         1886 g         2328 t
ORIGIN      
        1 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaaacaa aataaactta
       61 ttaaggaatg catcatgaaa cattcaatta ttgctgtcgc tgtcttatct tctgtattta
      121 tgagtgctgg tattttcgct gctgaagtag ataccggaac tttaaccatt aaaggtaata
      181 ttgcagaatc accatgtaca tttgtcgctg gcggtgattc agtcagtatt aatatgccga
      241 ctgtacccac cactgtattt gatggtaaag cgaaatattc aacttatgat ggcgcggttg
      301 gtgtaaccag cagtatgctg aaaattactt gtccgaagac tattgatggc gtaaaacttt
      361 cgttgatcac caacgagaaa attcttggta atgataaggc aatatccagt agtaatgaca
      421 cagttggata ctatctctat ttgggagata gcagtgacgt actggatatt tccgcaccat
      481 ttaatattga aagttataaa actgctgatg gacaatatgc aataccgttt aaggcaaaat
      541 atctgaagtt gactgacagt ggtgttgcat ctggtacagt taactcttca ttggttatgc
      601 gtgttgccca ggattaaata tttaaaataa tcatcttgtt ttttcagtaa gttaaaatgg
      661 ccgccatatt catggcggcc agcataaata taagaggcat taagatgaaa gggttattat
      721 ctttactcat ctcttctatg atattccccg tgcatgccgg aatggttatt ttcgggaccc
      781 gtatcattta cccggcagag cagaaagagg tcatggtaca actgatgaat cagggaaacc
      841 gctcttcgct gatgcaggcg tggattgatg atggcgacac gtctctgccg ccagaaaaga
      901 ttcaggttcc tttcatgtta acgccaccag tagtgaaagt agcggccaat tctgggcaac
      961 aaataaaaat taaaatcatg cctaataaat tacccagtaa taaggaaagt attttttatc
     1021 ttaatgttct ggatattcca ccgaatagcc cagaagatga aggaaagaat gcgttaaaat
     1081 ttgccatgca aaacagaatc aagttgtttt accgacctgc tggtattgct ccggtaaata
     1141 aaacgacttt taaaaaatta cgagtgagtc gtagcggtaa taacgtagtc ataaaaaatg
     1201 actcgccaaa ttgggtaacg atttctgatg ttaaggctaa taacgtcaaa gtgaattatg
     1261 aaacaatcat gattgcacca caagaaagtc agaatgttag tgaaaaaaat aataatgcga
     1321 atggctggca actgaccatt atcgatgatc acggcaacta tattagtgac aaaatttaat
     1381 tgcaggagct gcctatgttg agaatgaccc cgcttgcatc agcaatagtc gcgttattgc
     1441 tcggcattga agcacatgca gcagaagaaa cctttgacac ccattttatg atgggaggaa
     1501 tgaaaggtga gcaagtaaca aatttgcgtc ttgatgataa tcagccttta cctgggcagt
     1561 acgacattga tatttatgtc aataaacaat ggcgtggaaa atatgagatt atcgtcaaag
     1621 ataacccaca tgaaacttgc ctgacgcgtg aaattgtaaa gcggttaggt ataaatagcg
     1681 ataattttgc acgcgaaaat cagtgtttaa catttgagca acttgttcag ggcggaagct
     1741 actcctggga tattgggatt tttcgtttgg atctcgctgt tccgcaagcc tgggttgaag
     1801 agctggaaaa tggttacgtt ccgccagaaa actgggagcg gggaatcaat gcgttttata
     1861 cgtcttatta cgtaagtcag tattacagtg actataaagc ctcgggcaat agtaagagca
     1921 cttatgtgcg ttttaacagt gggctaaatt tgctgggatg gcagctgcat tcagatgcca
     1981 gttttagcaa aacggataat aatcctggtg agtggaaaag caacacgctg tatctggagc
     2041 atggattttc tcaaattctc ggtacgctgc gtattggtga tatgtacacc tcagccgata
     2101 tatttgattc cgttcgcttc accggggttc ggttatttcg tgatatgcag atgttgccaa
     2161 actcgaagca aaattttacc ccacgggtac aggggattgc ccagagtaac gcgttggtaa
     2221 ctattgaaca gaacggtttt gtcgtttatc agaaagaggt tccaccaggt ccgttttcga
     2281 ttagtgattt gcagttagcg ggcggagggg cggatcttga tgttagtgtt aaggaagccg
     2341 atggttctgt gactacgtat ctggtgcctt atgcggcagt acctaatatg ctgcaacctg
     2401 gtgtatcaaa atatgatttt gcggcaggcc gtagtcatat tgaaggtgcg agcaagcaaa
     2461 gtgattttgt ccaggcgggt tatcagtatg gttttaataa tttattgacg ctgtatggcg
     2521 gcacgatggt tgctaataat tactatgcct ttaccctcgg aacaggttgg aacacgcgta
     2581 ttggcgcaat ttcagtcgat gcaactaaat cgcacagtaa gcaagacaat ggtgatgtgt
     2641 ttgacggaca aagttatcaa attgcctata acaaattttt gagccaaaca tcaacacgtt
     2701 ttggtctggc ggcctggcgt tattcgtcgc gtgattaccg cacatttaac gattatgtgt
     2761 gggcaaataa taaagataat tatcgtcgtg ataaaaacga tgtctatgat attgccgatt
     2821 attaccagaa tgattttggt cgcaaaaata gcttttccgc caatatgagt cagtcattgc
     2881 cagaaggctg gggatctgtg tcattaagta ctttatggcg tgattactgg gggcgaagcg
     2941 gcagtagtaa agattatcaa ctgagttatt ccaataactg gcggcggata agctataccc
     3001 tcgcggcaag ccaggcgtat gacgagaacc acgccgaaga gaaacgcttt aatattttta
     3061 tatcgattcc ctttgactgg ggcgatgacg ttacgacgcc gcgacggcaa atatatatgt
     3121 ctaactcaac gacctttgat gatcagggtt ttgcttcaaa caataccgga ttatcgggaa
     3181 ccgttgggaa ccgcgatcag tttaactatg gtatcaactt gagccatcaa catcagggaa
     3241 atgaaacgac agctggggcc aatttgacct ggaccgcacc ggccgcaaca gtgaatggca
     3301 gctatagtca gtcgagtact tatcgacagg tcggagccag tgtttcaggg ggactggttg
     3361 cctggtctgg tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgcatg
     3421 cgccgggaat caaagatgct tatgtcaatg ggcaaaaata tcgtacaaca aactgtaatg
     3481 gtgtggtggt gtacgacgga ctgacacctt atcgggaaaa tcacctgatg atggatgtgt
     3541 cgcaaagcga tagcgaaaca gaattacgcg ggaaccgtaa aatgaccgcc ccttatcgcg
     3601 gcgcggtcgt tctggttgat tttgataccg atcagcgtaa gccctggttt ataaaagcat
     3661 taagatccga tggacagcca ttaacgtttg gttatgaagt caatgatatg catggtcata
     3721 acattggtgt tgtcggctag ggcagtcaga tatttattcg caccaatgaa ataccgccag
     3781 cggttaatgt ggcaattgat aagcaacaag ggctttcatg cacaatcacc ttcggtaaag
     3841 agattgatga aagtaaaaat tatatctgtc ggtgagaggt gaaatatgat gttcagaaat
     3901 agaatattac taatatttat attgtgggct aattttacct gggctgggtg tcgtactact
     3961 gcatcattaa atattacaga tggtattaat gttggggaga ttttagcgaa tgaaacttcc
     4021 tttagtaaaa gtgtcgtgtt tactgggata tcttgtgata cgagcacgga taaaatagtt
     4081 tataaaaata tccaaagtga ttgggttgaa gttgggcctt ttggtaatgg cgaaaaatta
     4141 aaggttaaaa tagagtcttt aggtaaaacc agcgacacaa ttgggaaatc cagcaatgcg
     4201 caggcagtat taccttatgt ggttaaaata gccagaggca cacctgattt tactggagaa
     4261 agaaaatcta cctggtttat ttcagatacc gtgattgcaa atattggcgg tgagtcatcg
     4321 tcatccatcg atttttggtt gggtatttgt aaggcattga agtttaactg gtgtgtgaat
     4381 tatctcacca gcaaactggc gggggataca tttacgcttg ggttaaatat ttcctattat
     4441 cctaaaaata cgacctgtaa gcctgaaaac accgttataa aagtagatga tatcgccttg
     4501 ttccagctca gaaatcaggg aaagattgcg gcgaacagta aggaaggaac aattacgttg
     4561 aaatgtgata atcttttcgg cgacaaaaaa caagcatcgc ggaatatggt tgtatatctt
     4621 tctagcagtg acttagttaa aggaagtaat actattttgc gtggtaaaac agataatggt
     4681 gtagggtttg tgttggatct aacagaacca ccaaaaggga ctgaggctgc cattaaaatt
     4741 tcggccaacg gcgatcaggg cgcggcgaca tcattatgga aaacagataa accaggagtt
     4801 tcattaaata gcaacattat taatatacca gtcatggcca gttactatgt atatgatgaa
     4861 aaaaaagtta aatctggcgc actggaagca accgcattaa tcaacgtgaa atacgattaa
     4921 taacaagcaa gatgagcaag tggctaataa taagaaataa taaggtgtct gcatttcccc
     4981 ctctctccga gggggctttt tttagcgatg ataaaaaatc tcaccgtcgt aggctttaat
     5041 gattttaccg tcggtgtcgc tgatcaacac gtacgcgcca cccatatagg tccagtgcgt
     5101 cccggcatcc ggcgcgggca ggttacgcaa ctgccactgc ttaatgttgt actcatcggt
     5161 acggtacatc tctggtacgg tgtcccctgg tttgtaatgc ttaaagtcag caatgaaact
     5221 gcttaactca tacttagaaa ttcccgaggt attggtcgaa cccgcggtgg ctggtgcggc
     5281 ccaggcggca ctggtaacca gcaaaagcgc acccagcaac atcttatttt taatggtcat
     5341 aaactctcca ctttggggat ctgtaatatc agtcgcatat ctttcctgcc atacaatata
     5401 actgacaagc gatattttgt tagtgatccc atgatttcgt aaaaagtgta aatgaaagga
     5461 tgtagcaaaa gcatcctcat tgagcgagaa gtgagcggaa taagctgatt taagaatcat
     5521 cctgggctaa aaggatagcg aagcccaggt gattgtttta tctcataata cccataaagc
     5581 catgcactcc catatacacc ccgactaaac caattaacag actggaaaaa tagggggcgc
     5641 gtttagcgag agtgttaaat ccgctccagc gttttgcaac ttgttgaaca ctgattgccg
     5701 ctccaacgcc tactgtaacc agcgttagtg ccagaccgat gctgaaactg acgaccagtg
     5761 ttgcgcccag tgtcagggct ttcaactgaa tgcaaatcaa cagcacggta attgctgccg
     5821 ggcagggaat aaggccacct gttaagccaa ataacaaaat ttgccagttg gtgacctctc
     5881 taccatcaaa gcgtcgttta atgtcattgg catgggctcg tgcatgggca tcctgatact
     5941 cttcgttgtc gccatgttca tgatggtgat gatgttcgtg gtcgtggtcg tggtcgtggt
     6001 cgtggtcgtg gtcttgatga tgttcgtgat cgtggtgatg atgctcatgc atattctcca
     6061 gccagttgcg ttcgccgcgc caggtacgcc agaacatcca gaaagcggtg ctaataatga
     6121 tcactgcgga aatcaactgg agccacggtt ctgctgattg agcagtaaag cgcttgctga
     6181 tcaccatccc gccaaaggca atcaaccaga ccactgcggt atgcgaaatg gttgctgcca
     6241 gtccgagcat caccgcctgt ttaatggtgc ctttgatggc gatgataaac gccgccatca
     6301 tcgtttttga gtgccctggt tccaggccat gaagcgcacc aagtaagatg gcgctgggga
     6361 tgaagaacca ggcgtttcct tgctgaagaa gagttgtaaa ttcggtcatg ataataattc
     6421 ttagtattaa ttcggcaatc tgattctact cccccccagt acctgatact accccccagt
     6481 agattaatag tgctatgatt tttcatgttc ttgttaacca ggtgttgcca tgtctcatac
     6541 aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag attcagggcc aggtcgtggc
     6601 gctcaagaaa atgctcgacg agccgcacga atgcgctgca gttttacaac agattgctgc
     6661 tatccgtggc gcggtaaacg gtctgatgcg ggaagtgatt aaaggtcatc taacggaaca
     6721 tatcgttcac cagggggatg agctaaaacg tgaagaagat ctggatgttg ttctgaaggt
     6781 gctggattct tatatcaaat gaaagtgcct aatatggccg tagttgaggc aataaataga
     6841 gggacaagtg acgttaagct gaattagagt aaatggcgtg aggacacacg ccattcgagg
     6901 ttatagtttg attaattttc gaaaaattgc ttcacagccc agccaccatg accacctgga
     6961 gttatgaatg aaacaaaacc tttgtcatct acgctctgga tttctaccgt cattgattca
     7021 tctaacacgc gacatttatt ggatgtaatg agactcagca tagcctgttg gtcattgtcc
     7081 tggatgtaac tgatcatctc gtaaaagtca gttaactttt tacagttaaa tgtaccttct
     7141 ttaagtgttg cgctgtctcc gtgacttagt gcgaatgcat ttggcatcat tgccacaaag
     7201 atgacggtaa cgagtagttt tttcatttta gcctcttata attcagaaca ttcactgatg
     7261 ttgtaagtgc cttttctacc atcatcgttt acgagatgta gcacgaagat gatgggattg
     7321 gcatcaatga aagatacaga gacgttcccg ctttcggtgc tatacatctt tgcaatctgt
     7381 ttttcaccaa tatatgagag atcccaactc actccgttca agctaaaatg catctgtttg
     7441 ccgtcaatat cgatggagga ttgtgccgta acggttttcc cattgctgcc aatttgaccc
     7501 tgctgaagag catcacattt ataggagctt gcataagtag atagaggtag tagcagaagt
     7561 gcagcaaaaa ttttactctt catattttcc ctttgcttgg atttgggtta cctattttgg
     7621 ggtagtcatt ataaaactat taattagtaa aacgaacaag ttttcatttg tttagcaatg
     7681 gtattgttta ggtttgaaat tattacgtat tttcatagca ataggttgga ttaacagcag
     7741 ttcagggaac aagttgcact atctacatac ttttccgctt tccattgcat gttaggtaat
     7801 agatagcctt tgcccgtaag actaaaaata ttagaaatga ctcattcgaa taccttctat
     7861 tcaccttaat ataggtttga ttattgtaat cgtttaatgt gtcttatgat tttttcctac
     7921 ttttcatttg cataatatag ggggcacagc atgctcttga aattaattat taaaatattt
     7981 aaattttaca gcacacaact acttttcgtt gtcttatgtg ttgaacgtac ttcgagtata
     8041 ggtagggttg tatatatatt gcgtgggtag agttagaata gaggaagcat catttataga
     8101 atattcaaga acgttagcct gtgat
//
LOCUS       DAZFDC010000169         8039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFDC010000169 DAZFDC010000000
VERSION     DAZFDC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.169"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(544..684)
                     /locus_tag="ACHJ48_004265"
     CDS             complement(544..684)
                     /locus_tag="ACHJ48_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4709700.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(986..1249)
                     /locus_tag="ACHJ48_004266"
     CDS             complement(986..1249)
                     /locus_tag="ACHJ48_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO4709701.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            1674..1799
                     /locus_tag="ACHJ48_004267"
                     /pseudo
     CDS             1674..1799
                     /locus_tag="ACHJ48_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            1864..2124
                     /locus_tag="ACHJ48_004268"
                     /pseudo
     CDS             1864..2124
                     /locus_tag="ACHJ48_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2174..2413
                     /locus_tag="ACHJ48_004269"
                     /pseudo
     CDS             2174..2413
                     /locus_tag="ACHJ48_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2461..3078)
                     /gene="ureG"
                     /locus_tag="ACHJ48_004270"
     CDS             complement(2461..3078)
                     /gene="ureG"
                     /locus_tag="ACHJ48_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO4709702.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLELIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3090..3764)
                     /locus_tag="ACHJ48_004271"
     CDS             complement(3090..3764)
                     /locus_tag="ACHJ48_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO4709703.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(3765..4229)
                     /gene="ureE"
                     /locus_tag="ACHJ48_004272"
     CDS             complement(3765..4229)
                     /gene="ureE"
                     /locus_tag="ACHJ48_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO4709704.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(4239..5942)
                     /gene="ureC"
                     /locus_tag="ACHJ48_004273"
     CDS             complement(4239..5942)
                     /gene="ureC"
                     /locus_tag="ACHJ48_004273"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO4709705.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(5935..6255)
                     /locus_tag="ACHJ48_004274"
     CDS             complement(5935..6255)
                     /locus_tag="ACHJ48_004274"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO4709706.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(6264..6566)
                     /locus_tag="ACHJ48_004275"
     CDS             complement(6264..6566)
                     /locus_tag="ACHJ48_004275"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO4709707.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(6657..7355)
                     /locus_tag="ACHJ48_004276"
     CDS             complement(6657..7355)
                     /locus_tag="ACHJ48_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO4709708.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGLLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            7736..8011
                     /locus_tag="ACHJ48_004277"
     CDS             7736..8011
                     /locus_tag="ACHJ48_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709709.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1944 a         2050 c         2035 g         2010 t
ORIGIN      
        1 ctgcaattat cgttttattc tttcactttt atcaaaacat caccagaaac tggaagtgac
       61 aaccgcaata atgaacataa aaaggcaaat cagaatggca gtaaatcaat aaagtaccat
      121 ttcatatgaa tcatcagaaa aacggtggac gcgacatcca gcacgaggca aaacacagca
      181 acaaaataag ccagatatcg cgccctgtac ttttctaact caactcccgg cattgtgcaa
      241 gttttaatcg caggtggatt cagagccacg cccccattga ttcagcctga agacacgtat
      301 atccttggag aaagcaggaa tctcattgct gtgaaattcc ttacagaatc ttcagcccca
      361 ttttatgggg ctttttttgg tgcccccacg ctgttaccac actttcggga tgcgaataaa
      421 aaagcccaca gtttttacac tgcggacttt tgttttttct ttgtgatttg tgtgcaaggg
      481 tgcgactttc gaccgaacct ttggcaaccg gatagcgggg actgttgtcc ccgctgcggt
      541 ttcttacggt ttacactgca agaacgccgc aatttccgac cacatgaagc ttaccctgaa
      601 ttcacacggt gatgcgtgta acacccagac ggtcagcaac atggtcaggc ataacatgcc
      661 cacaatgatc gttttttgcg gcatagcact tgctcctttt acagagaggc gctaaccttc
      721 cacttgctga aggtagatgg tcagggcctc gggttaacgt ttaagttgac tcggggcctt
      781 tccacatccg gccttcaggt aatccttcca gccgtcagcc gaaaggcacc cgcgcgtaat
      841 gtactggttt tcagagaggt ccgcaatctt ccggtgacga gatcatctgc acacgccccg
      901 gtgggacggg ctaacggtat attcaggtgc aggatctctc tgtctggtgc gtttttaaat
      961 tgtcaaacgc accctggcgg caaaatcagc cacctttttt cgcatcaaca aaacgcccaa
     1021 aacgctgggt aaagcgtgca acattacctt ctgaagcgac agttctcagt ttacctgtat
     1081 aaaacggatg cgacttagaa gaaacgtcaa ttgtaacata tgggtaagtt acaccatcca
     1141 gttcaattac ccggtcagtt ttgatcgtcg aaccgatcct gaagtactca tcaatactgg
     1201 tatcgtgaaa tactactgta cggtattccg gatggatatt cggtttcaca acagttttcc
     1261 ccctttaagt gaaaattccg cataaaggta catattcaga cggtcagatt caactgtctg
     1321 ttcttataac ctcactctct ttcagtatta caatatgtta tataattata aactggactt
     1381 cccgaaaaaa tccattaaat aagaagcgag ccgttgcgga ggcttgctct ctcctttaac
     1441 ctcatgtgca atatccgtaa cgcgctcaca taaatcatca tctgataata taacaacacc
     1501 cattatttta ttctgaccta aaaacttctt atgaaataaa cgtttacccg acgtatctga
     1561 tcccgctgtt tttacctgta tcctttaaca tacttgagaa gcccgctgaa aaaatatacc
     1621 gtaaaggcgg tttctctgtc ttccataaac ctgtcggata agaagggcat taaatgaaaa
     1681 ccaaaaaagt tatgatgatt gcactggtca gctctgcgct ggctctttca ggatgtggag
     1741 ccatgggcac agcgatcaaa aaacgaaatc tggaagtaaa aacccagatg agtgaaacct
     1801 gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta
     1861 ataatgagca gaaaaaccca acgttactct aaagagttca aagccgaagc tgtcagaacg
     1921 gttcttgaaa atcaactttc gatcagtgaa ggcgcttccc gattatctct tcctgaaggc
     1981 actttaggac aatgggttac cgccgccaga aaagggctcg gtactcctgg ttcccgcacg
     2041 gtggctgaac tggaatctga aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt
     2101 gagcgagata tattaaaaaa agcagctgta gattcaatct gtcaatgcaa cacccctttc
     2161 aattatctct ttcactgccc gcgacggtat cggttatcag tttgacctgc tggctaacgg
     2221 tgaaaccgta ctgcgtgatg catccggcga aaaacgcatc aagggtgaaa aagacggtcg
     2281 tatgctcatg aatgttggcc tgaatgcgga aattcgcgac aacatccgct tcggtctgga
     2341 gtttgaaaaa tcggcatctg gtaagtacaa cgtggataac gcggtgaacg ccaacttccg
     2401 ttattccttc tgataaaaac aaggggccgc ctgccggccc tgatacagag gatgttgtga
     2461 ttacttaccc agcatgccct gctcttcgat aaaggcgatg atgttttgca gaccttcacc
     2521 acttttcagg ttagtgaacc cccatgggcg ttctccgcgc atacgtaaag tatcacgctc
     2581 cattacctcg agggaggcgc ccacgtatgg tgcaaggtca gttttattga tcacgaggaa
     2641 gtcggatttg gtgatccccg gaccgccttt acgcgggatc ttttctcctt ctgccacatc
     2701 tatgacatat atggtaaggt ccgccagttc ggggctgaag gtcgcactca ggttgtcgcc
     2761 gccgctttcc acaaagatca gctcgagatt accaaatttc tcactcagtg cttccacggc
     2821 tgccagattc attgatgcgt cttcacgaat ggctgtatga gggcagcccc ccgtttcgac
     2881 accgacaatg cgttccgctt ccagtgctcc ggcctcagtg agaatgcgtt gatcttctct
     2941 ggtgtagatg tcgttggtga ctacggctaa ttgccaggtg tcacgcatgg atttgcagag
     3001 ggcttccaga agcgcggttt tacctgagcc gaccggaccg ccgacgccga cacgcagcgg
     3061 ttgcttgatt atgttcattt tattttctcc taagaacgga ataatcggga atactgggtt
     3121 tcatgtcgcg cggaagcgat agatgcaagt ggcgttgctg aaccaattgc gtgatccggg
     3181 gttgcaaggg cactgtccat gttcgccgca tagcgggcac acaggcgcat aattaactgc
     3241 tgggcagcca gctggccgta gggtaccagc ctgacgcccg ccatcactgc gctttcaatc
     3301 cagctgtagc caaggctcag ggccaggtct gaaacggata tttgccagcg tacgccgagc
     3361 catgccatac cggttaactg gctttgctgg catagtttgc gccattcggc cgggcagtcg
     3421 ggctgccagt cgaccagaag tctggtgaag gctgcaccgc ggttacgttc ttcatcgcgt
     3481 agctcacggg tttccctgca ggccagcaga taagcggtcc agcgcccggc actgtcagga
     3541 tcgcccgctt cacaggcccg gtatagccgg gcaagcagtg gcaggtcgac ggcaaaaaaa
     3601 ctctgttcca tctgcaactg ctgccagtgt tcaaaggcgg cgctatcttc tacccagcct
     3661 gcttccactg cccactccag tccctgggac cagctgtagc ctccaacagg gaggctgtta
     3721 ctggcaagct gcatcagccg cagacgtttt tccggagtcg gcattcagtg gctgtgctcc
     3781 tgatcgtgat tatgggcgtg cgacttgctg gcgtaagcgc cggcttccgg ctcaaacgga
     3841 agatgtgcaa aatcaacatc cagcccaaac tggcgcagca tatcatcgag gacgtgatcg
     3901 tgatggtagc gcagctctcc gggcattatt tgcagcggaa cgtggcgatt gccgagatgc
     3961 cagcaggctt tcgcgagcat aaaaggatct gcgcagcgca ctacagaaac ggcttcatca
     4021 gcggcgatca ccttaatgaa ttcatcaccg ttttcgttac tgagaatatc accatcgcgc
     4081 agcagcagac cgcgtggcag caacagtcca gcctgacgtc catcgttaag ggtgacttta
     4141 atacggcttt ttacccgcat gtcaacaggg agggtaacgc tggcagttgt ctgagccggg
     4201 gtctccaccc gccgggtcaa ataaagcatt tgtgctcctt aaaacaggaa ataacgttgc
     4261 gccattggca gaatgtccgc cggttcactg gttatcagtt cgccgttgat acgcacttcg
     4321 taggtttgtg aatccacggt tatatcaggc aacaggctgt tgtggcgcat atcggctttt
     4381 tgtactgtgc ggcagccttt taccacagca gttgtgctgt gcaggttaag ctgttcagcg
     4441 acgccatttg ctgctgctgc ctgcgacagg aaagtcacac gacagcggtg acgggcactg
     4501 cccaatgcag cgaacattgg gcgatagtgc accggctgcg gtgtggggat agagccgttg
     4561 atatcaccca tcggcgccat ggctatcatt ccgcctttga cgatagtcgc cggttttacg
     4621 ccaaagaacg ccggggacca gagcaccaga tccgccagtt ttcccacttc aatcgagccg
     4681 acttcatgag caataccgtg ggttaatgcc ggattaatgg tgtatttagc gatatagcgc
     4741 ttcacgcgga cgttgtcgtt atcaccactt tcttccggta acgggccgcg ctgaactttc
     4801 atccggtgtg ccacctgcca ggttcgtaac actacttctc cgacgcgtcc catggcctgc
     4861 gaatcggacg aggtgaggga gaacgcgcca aggtcgtgca ggacgtcttc cgcggcaatg
     4921 gtttcctggc gaatgcgcga ttcggcaaag gctacgtcct cggcgatatc cgggtcaaga
     4981 tgatggcaaa ccatcagcat gtccagatgc tcatcaatag tgttgacggt atagggtagc
     5041 gtcggattgg ttgaggaagg cagaatattg gggtgcgcgc aggcggtgat aatatccgga
     5101 gcatgaccac cacctgcacc ttctgtatgg aaggtatgga tagtgcgccc gccgatggca
     5161 gtcagagtat cctcaacgaa tcctgattcg ttaagcgtat cactgtgtag cgcaacctga
     5221 acgtccattt cgtcggctac agtcagtgcg cagttgatta ccgcaggtgt agctccccag
     5281 tcttcgtgaa ttttgaggcc gataaccccg gccgcgacct gctcacgaag cgcatccgga
     5341 ttggagcaat tgcctttacc cagcaacccg atattgaccg gcaggctgtc tgcagcctgc
     5401 agcatttgat aaatgtacca tggtcctggg gtacaggttg tggcgttaga acccgctgtc
     5461 gggccagtac cgccaccgat catggtggta atgccggatg tcagcgcttc ttcagcctgc
     5521 tgtgggcaga tccagtgaat atgcgtatcg acgccacctg cggtaacgat cctgccttct
     5581 gcggcaataa tttccgtgga tacgccgatt gggatagtga cgttgggttg tatatcagga
     5641 ttaccggctt tgccgatagc aaatatcctt ccatctttga cgccgatatc ggctttaacg
     5701 atcccccagt aatcgatgat cagggcattg gtcagcacca gatcagcgca gccggcggag
     5761 agcatttgcc cctgtcccat accgtcgcgg attactttac cgccgccgaa tttgacctct
     5821 tcgccgtagg tagttaaatc atcttcgacc tcgatccaca gctcagtgtc tgccagacga
     5881 attttatcac cggtggtagg gccgaacatg tcagcatagg cctggcgtga aatattactc
     5941 atcatttgcc tccagctcac ccattatatc tccgcggaaa ccgtgcacga tacgggcacc
     6001 gctcagcgcc accagttcaa cctcacgctt ttggcctggt tcgaagcgca ctgcagttcc
     6061 ggcggcgata ttgagacgat agcctcttgc cttctggcga tcgaacttca gtgccgggtt
     6121 aacctccgcg aagtgataat gggaaccgac ctgaattggt cggtctccgt gattttcaac
     6181 gataatactg caggtagccc ggccgatgtt aagctctata tagccaggtt taaccttata
     6241 ttctccaggg atcatctgtt acctcagatt atcggattat ggacggtaac cagcttggag
     6301 ccgtccggaa aggtggcttc gacctggata tcggggatca tttctggtat gccttccatg
     6361 acctgctcgc gactcaggac atgccgtcct tcttccatca gcgcagcgac gcttttgccg
     6421 tcgcgagcgc cctccattat aaaagcgcta atcagggcca cggattcggg atagttaagt
     6481 ttcaggccgc gggccagacg acgctctgcc agcagtgcag cggtaaacag taatagtttg
     6541 tctttttctc ttggagtcag ttccatagcg ttctcttaag tcagccagat ccggggaagt
     6601 accggtgatt tgtcagtcag ttgcggccgc ataaattgcc agatatcacg cattacctac
     6661 tggcaacgca gattatcatc acttaaaaag cgaactgtca gtagaccatc ggtgagcgtg
     6721 gctccggcgt agttttccag tggtgtgagt ttttcgcgta tgtcttccag ttgcgtttca
     6781 ttccccaggt agaacagcat cgttcccacc catggtttcc gggcaacgca ggtaagttct
     6841 ccattggcca cgtgcagacg ctcaatcaac agcggagaac cgtcgaccca tatctccaga
     6901 cggttagcga gtgttccgtg gctgaaggtt tctccgatga ccggacgtcc aaggcacaga
     6961 agatcccagg ccagcagtgt actggaggac gacagatgaa acacggtatg caacgtggcg
     7021 tgagcgccgg gaaaaaaaat cgaatcctgt ggcaaccact ccagcgttgc gttattgcac
     7081 agcgtcaggt tttgctgaag gtgcgcctgc gcgccgctgc tgcggtaaaa tttactggca
     7141 ccaggcatgg tgatcagcgt atggcaatca gagtcaatta ttgcgttgat gatgagttcg
     7201 tcaccgccga cgataccacc gggtggatga agcagacaga ggtggcaggt ttcttcttcg
     7261 ggataaaaag gacgctgaac agtgagcaga ccgacatgat gacgggtggt gagcgtggtt
     7321 ttcccgtgca gacgctgaaa acggagatcc agcatggcct gccagccttt tttattttgt
     7381 ggtaacgcgg tactgagcaa tcttacgccc ctgataaata aagataaaac gacagtaaag
     7441 agccggattt ccttgttata taaaagatga tgcctttcca gtgagttttc gtctgaataa
     7501 tacgaatgtt tgttttcgtc atgaagcgtt ccggctcccg gggctataca tcgccagaaa
     7561 agcaatgcat attgtcggga ttatccagag aatcaggcag aaagatccga caatgggctg
     7621 tgattatgcc atctggctca gaatgatatc aaggtgttat ggggataact tgctgataca
     7681 atagcagtat gacagattca ttaatgcgga aactgctcac tgaaacagag tgttcatgac
     7741 tgagcactgg tcgggtcatg cagatgataa tttccccttt tcttggggaa gcgacagtat
     7801 tttcatgcat gcctgtcttc agtcatacgg ggagagcatc tgtgatacag tccatacatt
     7861 tgttgcggac gtcgctcctg agtcactgac tggtctgcaa acagtttatc tgtacacggc
     7921 agggcttctg tgtgatatcg tttttcatat cgaggggggg cctccagaga tgaatggaat
     7981 aataatattt cctgatgata taatacctta gagaatttct gtgtatgcta ttgtgaaca
//
LOCUS       DAZFDC010000170         8035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFDC010000170 DAZFDC010000000
VERSION     DAZFDC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.170"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(142..999)
                     /gene="murI"
                     /locus_tag="ACHJ48_004278"
     CDS             complement(142..999)
                     /gene="murI"
                     /locus_tag="ACHJ48_004278"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO4709710.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(944..2788)
                     /gene="btuB"
                     /locus_tag="ACHJ48_004279"
     CDS             complement(944..2788)
                     /gene="btuB"
                     /locus_tag="ACHJ48_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO4709711.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGGSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2853..3043)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3157..4257
                     /gene="trmA"
                     /locus_tag="ACHJ48_004280"
     CDS             3157..4257
                     /gene="trmA"
                     /locus_tag="ACHJ48_004280"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO4709712.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4297..4656)
                     /gene="yijD"
                     /locus_tag="ACHJ48_004281"
     CDS             complement(4297..4656)
                     /gene="yijD"
                     /locus_tag="ACHJ48_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO4709713.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4656..5306)
                     /gene="fabR"
                     /locus_tag="ACHJ48_004282"
     CDS             complement(4656..5306)
                     /gene="fabR"
                     /locus_tag="ACHJ48_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO4709714.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5410..6726)
                     /locus_tag="ACHJ48_004283"
     CDS             complement(5410..6726)
                     /locus_tag="ACHJ48_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO4709715.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6769..7935)
                     /locus_tag="ACHJ48_004284"
     CDS             complement(6769..7935)
                     /locus_tag="ACHJ48_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO4709716.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2111 a         2175 c         1900 g         1849 t
ORIGIN      
        1 cggaattctc aggagaatcc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagagg aaatttaaaa taattttccg
      121 accgcgcaac attcaaccaa atcagcctaa aactgccagt ttttcgagcg tttcgaagcc
      181 gtaacgctgt aaaacgggca ataattgttc agcttctggc gtcattgcca tacaaaaggc
      241 aatattcgca tcggcagatt ttgcatccgg ggcttcatgt tctaacaacc aggccgttcg
      301 gcgagcaatc gctgcgccag aatccaccag ccgggttccc tctggcagca cttgtaacag
      361 ttcttcttgt agtagaggga aatgggtgca acccaataca acggtatctg gcggctcttt
      421 cattcttaac cacgggcgta ggatacgttt tagtgcatcc agagaaacat cttcgccatg
      481 cagcttcgct tcagccaact caaccatctc tgccgagccc agcatttcta tctggcattc
      541 attagcgaaa cgcgcgatca gctcatgagt ataagaacgt ttaactgttc cgcgggttgc
      601 cagtaatccg acaatgccat ttgccgtcag acgtgcagca ggtttaatcg ccggcacgac
      661 accaacaacc gggaagtcga acttttcgcg taatgcagga agtgaaacgg tactggcagt
      721 gttgcaagcg accacagcca gcgcaagggg ataacgttct tgcaccgcgg tgacaattgc
      781 caccactcgc tcaacaataa acgcttcgct tttttcgcca tacgggaaag cgacgttatc
      841 gaaagcataa atgtaatgga gatccggtaa gagatgccgg atctcgtcat agaccgacaa
      901 cccaccgacg ccggagtcaa acaccagcac ggtgggacgt ggttcagaag gtgtagctgc
      961 cagacaaggt gtattcccgt cctgcagttt ggtagccata gactgtctca taatctttgt
     1021 cgaacaggtt ggctatttta ccacgaactg tcaggtgaga ggtgaccgga tacgcaaccg
     1081 caagatccca caagctcaca ccgcccattt taacggtttg ataaggataa gatgagtaat
     1141 ccttatcata gcgagtgcct aaatactgat aagtaatacc ccagtcgaag tcatacaact
     1201 gccagtcgag ctggtatttc acctgctgtt tagcacggcg taacaacggc gtgtcggtaa
     1261 ttgcattgcg cgcatcgaca taatcataac tcacagtatg cgtcagtggt ccggtatcaa
     1321 aattggcggt cgcctcgacg cccttaatcc gcgctttccc ttcgttgtaa tatttcaggg
     1381 tgtgatcatc ataatcgatc aagtcactga catcgttacg atatccggaa atacgccagt
     1441 tcaccccagc ggttaagcct tcaaacgcgc cttcccactg tttgcttttc tccgggtcca
     1501 gattcggatt tccgtagaag ccatacagtt gccccagatt tggtgcctta taagatgtcc
     1561 cgtaggaagc aatgaagcga taaccttcga tgaattccca accggcgctg gtttgccagg
     1621 ttccatgacg accaaactgt gagttatcgt cgctgcgtgc tgcgccttca aaggtaaaat
     1681 cgccgacttg ttgcagcccg gtcagataga tgccggtatt acgttgatca tatccatcct
     1741 taacataagc tgtgcccggt gccgtgctct gcttctgcca gtcaacaccc gcaccaacat
     1801 taccgtggcc aatgatgatg ttgtttgccc actggacggt gtattgcttc atctcatcga
     1861 gcgtcgccga cgaatcataa cgaccataat ggggatcgta gttgtaatct ttgctatggc
     1921 tatagctggt aatgagttgt gatttaatca gttcgccgtt atatcgcagc ccggcgtccc
     1981 aactttgact atagagttta cgggtatcga ccaatggtga acccggagaa taatacgcgt
     2041 cataattggt acggttatca tagccatagc cgcgcacaaa gccgctccag gcatcagtaa
     2101 agttatgctc cagcgcgcca taaagcgttt tacttaaaaa accatcgtta tctggctgcg
     2161 cttgcgttcc ggtattacca taggcaacaa catcataacc atgagtatgg gcataatcgc
     2221 ccaacaacgt tactcgtgtc ttatccccca gttgttgctg tgtagagaca tcatagtttt
     2281 gataactatt gcttccccac cctgctgaaa tttccgttcc gggttcatcg cgcgtcgtga
     2341 tgatattcac caccccacct attgcatcgg aaccataaac ggcggagcgt ggcccacgga
     2401 tatattcaac acgctggaca agcgcaatag ggaactggct aaggtcggca gaaccactcc
     2461 cccccgccag attcaggcgt acgccatcaa ttaacaccaa cacatgactg gcatttgtac
     2521 cgcgaataaa aatagatgag agctgacctg aaccgccgtt ttgggtgata tcgacgcccg
     2581 gaagacggcg cagcacatca ttaaccgagg tcgactgcca gcggtcgata tcctgacgcg
     2641 tcacaacggt ggttggtgca agcacagtgc tgcgcggctg ttcaaaacgg ttagcagtaa
     2701 cgacgagagt atccgggctg gtatcctgtg cccaagcgga aaaggctgtg acagaacacg
     2761 ccgtcagcag cgaagctttt ttaatcattg taaagcatcc acaatagaag aaggatgccg
     2821 caggtttcat caatattacg cgatgatgag aaccagatgc gacgttggcc ggcaggtctt
     2881 cgggcttgga ggggtatcta agatactaag agatgatgac ttcccaccga atggcagtgt
     2941 ccgcataacg caatcatcgc acctttcctt accgctgcgc gtcagctcca gattcgcact
     3001 ggattcccta ttaactcaca ggaccggcaa gtggatgcta caggttgtaa caagttactg
     3061 tccagacgta gctcacaaat aggaattcat caagatctgg acatctgatg agcaatccct
     3121 acaatcgccg cgtactttaa tttttcagga tacatcatga cccccgaaca ccttccaaca
     3181 gaacagtatg aagcgcagtt agccgaaaaa gtggtacgtt tgcaaagtat gatggcaccg
     3241 ttttctgacc tggttccgga agtgtttcgc tcgccggtca gtcattaccg gatgcgtgcg
     3301 gagttccgca tctggcacga tggcgatgac ctgtaccaca tcattttcga tcaacaaacc
     3361 aaaagccgta tccgggtgga tagcttcccc gccgccagtg aactcatcaa ccagttgatg
     3421 acggcgatga ttgcgggtgt gcgtaataat cccgttctgc gccacaagtt gttccagatt
     3481 gattacctca ccacgctgag taatcaggcg gtggtttccc tgctgtacca taaaaagctg
     3541 gatgatgagt ggcgtcagga agcggaagcc ctgcgcgatg cactgcgcgc gcagaatctg
     3601 aatgtgcatc tgattggtcg ggcaacgaaa accaaaatcg cgctggatca agattacatc
     3661 gacgagcgtc tgccggtcgc agggaaagag atgatctatc gtcaggtaga aaacagcttt
     3721 acccagccga acgcggcgat gaatattcag atgctggaat gggcgctgga cgtaaccaaa
     3781 ggctcaaaag gcgatttact ggagctgtac tgcggcaacg gtaacttctc attagcgctg
     3841 gcgcgcaatt ttgatcgggt attagccacc gaaatcgcca agccatcagt cgctgccgcg
     3901 caatacaaca ttgccgctaa ccatattgat aacgtacaaa ttattcgtat ggcggcagaa
     3961 gaatttactc aggcgatgaa tggcgtgcgc gagtttaacc gcctgcaagg gatcgactta
     4021 aagagttatc agtgcgaaac catttttgtc gacccacctc gcagcggtct ggacagtgaa
     4081 accgagaaaa tggtgcaggc gtatccacgt attctgtata tctcctgcaa tccggaaacg
     4141 ttatgcaaaa atctggaaac attaagccag acgcacaagg tcgaacgtct ggctctgttt
     4201 gatcagttcc cctacacgca ccatatggag tgcggcgtat tactgaccgc gaagtaaaac
     4261 ccatgccgga tgcgccagca tccggcatga taccgattac tcagcaactt cctgcttacg
     4321 gttacgcatc ttcgcgccaa tccagaacac cataatgacg gagagtactg ccgggaagaa
     4381 gttagagccg atatccggat attccgcacg aactaccgtg ctgtacagca gtacgccgag
     4441 aataaaacag gcagctgcca gacccggcaa gcctaccggc atggtgcgat taagataacg
     4501 ttgatgcagg cagtaaaccg tcagcaccag ggaaataatc gggaatacag aaaatggcac
     4561 aatggagcta aacagcgctg cgaaagtacc attaatcgat aagccagcaa ccaacgccag
     4621 cagcagcgta cctctatctt gatttgcttg tttcattact cgtccttcac atttcccgga
     4681 ataattgcgg ttttctcttg ttcacggcga taccagtaat aagccccttt cgaaatcatt
     4741 cgcagttgca gtaccagtcg ctcttctaat tgccgacgtt gttcaacgcc gacgtccaac
     4801 gcctcggcac ccgcactgaa gacaattgtc accattgctt cggcttgcgc ttcagtaaac
     4861 gcacgcggca tatggttttc gagttccaga tagtccgcaa gttccgcaat gaagtgctga
     4921 atttcacgcg caacggcggc acgaaacgca gcggaggtgc cggagcgttc ccgcaataat
     4981 aaccggaagg cgttaggatt attaccgatg aactccataa atgtggagac cgaggtgcgg
     5041 atcacactcc cgcctttggc gatacgctga cgcgcctggc gcatgagttg gcgtagcatt
     5101 aaaccgctct catcaaccat ggtcagaccc agttcgtcta cgtctcggaa atgccgataa
     5161 aaagaggtgg gagcaatgcc cgcttcacgc gccacttcac gcaaactcag gctggcgaag
     5221 ctgcgttcag cacttaattg gctaaatgcg gcttccacca gcgaacggcg ggttttttct
     5281 ttttgttgcg ctcttacgcc catcacgatg tctgaatcct tgccaaaagt actgctggca
     5341 ctataccaga gaatgaacat actggattgc tccagtattg tgaatgaacg gtaacgcaat
     5401 aaaacatatt tactgcaatt ctttggccgg ttcttttacg tacaacaacg ccaacatcga
     5461 cagcaacgtg cctccaccca gataccacgc cggagccgtc aaactatcca gcaacacgat
     5521 ggattgcgta gcgaaatatt gagcaaaacc gccaaagatt gccacaccaa tgctataaac
     5581 cagcgcaaaa cccaatgccc gaatccgttt cggcaacagt tcagagatca acaacatcac
     5641 tggcacaccg ccaagcgtgg taaagctgac catcacgaaa accaccgtca gcaacatgcc
     5701 tgggcttggg taattcacca acagccagaa tgacggccag ctacagatca gcaccatcac
     5761 acgggagatt aaaatcagct ttttacgccc gaccgaatcg cacaacatcc ccaccagcag
     5821 cgcacccaca aacgtaataa cgcccgccag taacatcgct gcatgagaat agttttggtt
     5881 cattcctaaa tacttcgccg cccatgtacc gtaataaaag agggagatat aggttgcgac
     5941 tgtactacca atagcgagca agataccgtt agcgatagtc gctttatgtt gcataagtaa
     6001 ggagaaggcg ctttcgctgg ctgcggcctt cttattatgt gctggctcag gaacatcatt
     6061 ttccagactc aaacgcaacc aacagccgac aggcgctaat aacacgccaa taaaaaacgg
     6121 cacacgccag ccccattcag ccatgacggt ttctgaaccg gtcgcaaagg gcagccatgc
     6181 acttaaacct aaagcgacaa cacctccaaa agttgttgcc aggccttgag tcgccagtga
     6241 ccaactgctg taaaaaccgc gacgatttgc cggagctgat tcaaccaaca aagacattga
     6301 ggcaccgact tcgcctcctg ccgcaacgcc ctgaattaga cgcgctagca ccagcgtcgc
     6361 ggttccccaa tagcccgccg tttcatacgt cggtgcgatg ccgatcatca gtgtaccaag
     6421 actcatcagc gcaatggtga acaccatcgc gggtttgcgt ccgaaacgat cggcccaggc
     6481 ccccacaata atgccgccga gcgggcgcat aaaaaagctc acgccgaaag tggcaaacgc
     6541 cagcagtagc gcgacgtttt tatctgcact gggcggaaaa aatagcgtgc tgatatagac
     6601 cacaaaaaag ttgtaaacgc caaaatcaaa aaattcgagc gcattgccaa acgccgaggc
     6661 aaaaatcacc cgcctgccgc tgggcttttt cttcacactc acggtttcat ttacgatcgc
     6721 cgtcattcca tgttccttgc caaaaagtgt tgttgtgtgt tgttttattt attgcagcca
     6781 ggcttctacc agtgcgcccc agtagctgct tgccgggacc agacttgcat cgttgaagtc
     6841 gtaacccggg ttatgcacca tgcaggaacc ttccccgaca tcgccattac cgaataacag
     6901 gaaaccgcct ttgggttggg cttccagcat gaaagagaaa tcttcactgc ccatcagggg
     6961 cttgatgcca aactcggccc gatcttcacc aaacgtttta cacgccacct gccaggcgaa
     7021 gcgcgccatc tcctcgtcat ttactaatac tggtgtaccg ttaacatgcg taacctcagc
     7081 cgtagcgcca aagctggcag cctgggcttg tgccagagca ggaatacggg tcagaagaag
     7141 ttgtcgggta tcgcggctaa gagagcggac gctgattttc atctcagcac tgtcaggaat
     7201 gacgttagcc gcctcacccg caacaatgct gccgacggta attaccgccg cttccagcgg
     7261 atcgacattg cgtgacacaa tgctttgtaa tgcggtggtg atatgtgcgg caactagcac
     7321 cggatcgatg gctttgtgcg ggatcgcacc gtgtccgcca caaccacgaa ctgtaatatg
     7381 gaactgatcc attgacgcca tcagtgcccc tggctggaaa taaaacttac ccaccggcag
     7441 accgggcatg ttatgcatgc cgaaaatgac atcgcagggg aaatgatcaa aaagcccctc
     7501 tttaaccatg atctcaccgc cattaatcat ctcttccgca ggctggaaga taagacgtaa
     7561 cgtgccgtta aaacggcggg tttcggcaaa ataacgcgcg gcccccagca gtatggtggt
     7621 atgcccgtca tgcccgcagg cgtgcattaa accaggatgt ttactggccc agggcttgcc
     7681 actattttca taaatcggca gcgcatccat atccgcccgc aggccaatac ttttctctcc
     7741 gtcgcccact tttaacgtgg ccactatccc cgtcgtcgcc agtccttgct ctacttcata
     7801 gccccactga cgcagctttt ccgcaaccaa agcactggtt ttaaattctt gtaatcccag
     7861 ctccgggttt tcgtggatct gatggcggat ctcacgcagt tcttcctcaa accctcgaat
     7921 atagtgttcg atcatcggct attcctttat tcacttataa acaggctaat taacggattt
     7981 tattgatggt gtaaatacaa ataactcatc aatataaaat atttatatgt ttgtt
//
LOCUS       DAZFDC010000171         8035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFDC010000171 DAZFDC010000000
VERSION     DAZFDC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.171"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            42..2132
                     /gene="iha"
                     /locus_tag="ACHJ48_004285"
     CDS             42..2132
                     /gene="iha"
                     /locus_tag="ACHJ48_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO4709717.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(2350..2500)
                     /locus_tag="ACHJ48_004286"
                     /pseudo
     CDS             complement(2350..2500)
                     /locus_tag="ACHJ48_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2712..3032)
                     /locus_tag="ACHJ48_004287"
                     /pseudo
     CDS             complement(2712..3032)
                     /locus_tag="ACHJ48_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(3455..4030)
                     /locus_tag="ACHJ48_004288"
     CDS             complement(3455..4030)
                     /locus_tag="ACHJ48_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4709718.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(4099..4677)
                     /gene="terD"
                     /locus_tag="ACHJ48_004289"
     CDS             complement(4099..4677)
                     /gene="terD"
                     /locus_tag="ACHJ48_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO4709719.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(4726..5766)
                     /gene="terC"
                     /locus_tag="ACHJ48_004290"
     CDS             complement(4726..5766)
                     /gene="terC"
                     /locus_tag="ACHJ48_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO4709720.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(5789..6244)
                     /locus_tag="ACHJ48_004291"
     CDS             complement(5789..6244)
                     /locus_tag="ACHJ48_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO4709721.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(6267..7424)
                     /locus_tag="ACHJ48_004292"
     CDS             complement(6267..7424)
                     /locus_tag="ACHJ48_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4709722.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(7424..8005)
                     /locus_tag="ACHJ48_004293"
     CDS             complement(7424..8005)
                     /locus_tag="ACHJ48_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO4709723.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         2031 a         2049 c         2026 g         1929 t
ORIGIN      
        1 gtgtattgtc ttgccggtta acatgatcgg agattagtaa tatgcgaata accactctgg
       61 cttccgtagt cattccctgt ctcggatttt cagccagcag catagctgct gcagaggatg
      121 tgatgattgt ctcggcatcc ggctatgaga aaaagctgac taacgcagcc gccagtgttt
      181 ctgtgattag ccaggaggaa ttgcagtcca gccagtacca cgatctggcg gaggctctga
      241 gatcagtaga gggtgtggat gttgaaagtg gtacgggtaa aaccggaggg ctggaaatca
      301 gcatccgagg aatgccagcc agttacacgc tgatactgat tgatggtgtt cgtcagggcg
      361 gaagcagtga cgtgactccc aacggttttt ctgccatgaa taccgggttc atgccccctc
      421 tggccgccat tgagcgtatt gaggttatca gggggccgat gtccacactg tatggctctg
      481 atgcgatggg cggtgtggtg aatatcatta ccagaaagaa tgcagacaaa tggctctctt
      541 ccgtcaatgc agggctgaat ctgcaggaaa gcaacaaatg gggtaacagc agccagttta
      601 atttctggag cagtggtccc cttgtggatg attctgtcag cctgcaggta cgcggtagca
      661 cacaacagcg tcagggttca tcggtcacat cactgagcga tacagcagcc acgcgtattc
      721 cttatcccac ggagtcacag aattataatc ttggtgcacg tcttgactgg aaggcgtcgg
      781 agcaggatgt gctctggttt gatatggata ccacccggca gcgttatgat aaccgggatg
      841 ggcaactggg gagtctgacg gggggatatg accggaccct gcgctatgag cgaaacaaaa
      901 tttcagctgg ctatgatcat actttcacct tcggaacatg gaaatcgtat ctgaactgga
      961 acgagacaga aaataaaggt cgtgagcttg tacgcagtgt actgaagcgc gacaaatggg
     1021 ggcttgccgg tcagccgcgg gagcttaagg aatcgaacct tatcctgaat tcattactgc
     1081 ttacccctct gggagaatct catctggtta cggtgggggg cgagtttcag agctcgtcca
     1141 tgaaagacgg agttgtcctt gccagcacag gtgaaacttt ccggcagaaa agctggtcgg
     1201 tatttgctga ggatgagtgg catctcacgg atgcacttgc gctgactgcg ggcagccgct
     1261 atgaacatca tgagcaattc gggggacact tcagtccgcg tgcatatctg gtctgggatg
     1321 tggcagatgc ctggacgctg aaaggcggtg tgaccacggg atataaggca cccagaatgg
     1381 ggcagctaca taaagggatt agtggtgtgt ccgggcaggg aaaaacaaat ctacttggta
     1441 accccgacct gaagccggaa gagagcgtca gttatgaggc tggggtgtat tacgataacc
     1501 ccgccggtct gaatgccaat gtcacaggtt ttatgactga cttctccaac aagattgtct
     1561 cttattccat aaatgataac accaatagct atgtaaacag cggaaaggcc cggttgcacg
     1621 gtgtggaatt tgccggcaca ttgccgctgt ggtcagagga tgtcacgctg tcactgaatt
     1681 acacctggac ccgaagtgaa caacgtgatg gtgataacaa aggtgcgccg ctgagttata
     1741 cccctgaaca catggtgaat gcgaaactga actggcagat caccgaagag gtggcatcat
     1801 ggctgggtgc ccgttatcgc gggaaaacac cacgtttcac ccagaattat tcgtcactga
     1861 gcgctgtaca gaagaaagtg tatgatgaga aaggagaata cctgaaagcc tggacggtgg
     1921 tggatgcagg tctgtcgtgg aagatgacgg atgccctgac gctgaatgct gcggtgaata
     1981 acctgctcaa caaggattac agtgacgtga gcctgtacag tgccggtaag agtacgctgt
     2041 atgccggtga ttacttccag acgggatcat caacaacagg atatgtgata cctgagcgaa
     2101 attactggat gtcgctgaac tatcagttct gataataaca aaacgctatc actgacggta
     2161 gaatacgttg ccactgcaac tcctggcgga acagtggcaa cgtattaggt taagtgcatt
     2221 tccgatccgc taatgagatt tcgttaccaa caactaatat cgtcacagga aatgcacgga
     2281 ttatttttaa cttatcattt acatacttgt ccagagtgta agcgcaccgt gaaggacgtg
     2341 gggtaaaaat tagtttacag agagagtgac gttccagggc aacaactctt tcatgcggtt
     2401 ggcaggccag gtgttgatta cactgatcac gtggcgtagc cacgtttccg gctcgattcc
     2461 gttaagtttt gcagctaccg atcaggctgt acatcactgc cgcactatcg ctcgtcatct
     2521 caaagtcctg tctcgtcagc aggaaggtat cattctctcc cgccattttt ccaggggccc
     2581 ggtcagataa gtccctttgt ctatcgctga ctcctgactc ataacccggt tagcagaatg
     2641 cagggtcacc acctcgccac gaccaaatcc aaataagtca attgcacctt ctcaatcgcc
     2701 attttgtcag taagcgtaca gcctgaactg atggtatctt caccatcaat gacaacggtg
     2761 atcgcaattt tactgacgtt cgccggaaca cgatccagtg ctatctcaat gctggcctgc
     2821 tgcgaaccgg taacgagcct gactgccccc tcaggagaag acaaattatt ataaaagata
     2881 aagtcagaat cgccactgac ctttccctga gcattaagca tgaacaggca ggtatcgggt
     2941 tcgcctttaa agccggattt ggcagggtac tgaagattca gcctgatcgt agattgctga
     3001 aggggtatgt tttgtccgga ttgtaaattc atattaactc tcctgatttt tgattattat
     3061 taatgcgcag cgtttatata tgttccctag gcttagttct ggacgctgga tattcggtga
     3121 ggcgtaaata tggtatgaca ccatttttca taacgctgaa gtttctatac ctgttgaatt
     3181 tgaattttca ttgaccgggt atcttatttt ccagggcccg ttccttcata agtcgcaaaa
     3241 gtaacatata tccgaagggc atgctgttga tatcagacac ggaatactgg ctttaaccag
     3301 caaccacaga taaacccggg gcctgcataa aaggttcatg ccagaattaa catagctctg
     3361 ctttctgccc cccccctttc ccatattcac ccgttgatag cggatcgata cccaaaaaaa
     3421 accccggcat tgccggggtc agactcagcg tcttttagac attgacgccg tgctgggaag
     3481 caagcgctga cagaccaccg gcaaaaccct ggccaacagc tttaaacttc cactcagtac
     3541 catggcgata cagttcaccg aagaccattg cggtttcggt tgaggcgtct tcagacagat
     3601 cgaaacgggc aatttccgtc ccgttgtcgt tgttgtaaac gcgcatgaag ctgttgctca
     3661 ccatgccgaa gttttgttta cgcgcttctg catcatagat ggtaacggca aataccagtt
     3721 ttttgatgtc tgctgagact ttggtcagat cgattttgac ctgctcatcg tcgccgtcgc
     3781 cttcaccggt acggttgtcg ccctggtgct ctactgcgcc atcagggctg gttttattat
     3841 tgaagaaaat gaaatgggca tctgacagta ctttaccgtc ttcacctact gcgaatacgg
     3901 aagcgtccag atcaaaaccc tgaccatcgg ttacacgggc atcccagccc aggccaacca
     3961 tagcgacatt catggttggt gcttctttgg tcagagatac gttgccgcct tttacgagag
     4021 aaactgccat ttttagctcc tgcaaacagt tgaatgaggc tgaattaaca gccccagata
     4081 tgaaaagtta cttttcgatc aggacgcgtt aatgccgtac tgagcacata cagatgccag
     4141 accaccagca taaccctggc ctactgcgcg gaatttccac tcaccattgt ggcgatacag
     4201 ctcgccgaac agcatggcag tctcagtgga cgcatcttcg gtcagatcgt agcgagcgac
     4261 ttcagtctgg ttatcgtcat taaccagacg aataaacgca ccggatacct gaccaaagct
     4321 ctggcgacga gcctgagcat cgtggatggt cacaacgaag atgatcttgt caacttcaga
     4381 cgggacggcg tccagtttaa ttttcagcga ttcatcatca ccatcgccct caccggtgcg
     4441 gttatcgccg gtgtgcgtta cggaaccgtc ggatgacgtc aggttgttat agaagatgaa
     4501 atctgaatcg ccgcgcactt tgccgtttga ggccagcagg aatgctgaag catccaggtc
     4561 aaagtcctga ccgtctgttg aacgcgcatc ccagccaagg cccaccagga catttttcat
     4621 tgacggagct gctttactca gggagacgtt cccgcctttg gaaagagaaa cactcataaa
     4681 ataacctctt cgattagtaa ttgttcaggt taacacttaa ggggattaac tccccttttc
     4741 ctcagattca ggtttgcccg ggaacatgac gcttgcgaga atgcccagcg ccagtacacc
     4801 cagtacaaca tacaggctgg ttgttgccgc gatgctgtaa ccatgatgcc agatgtgatc
     4861 ggtcgcattc aggccgagtt ttgccgcgat gaagaacagc agcacgataa cggccttctc
     4921 cagatgaacc aggtactgtt tcagtgcctc aaggacaaaa tacagagtac gcagacccag
     4981 gatagcaaac atcatggcac tatagacgat aagcggttca cgactgacgg caatgattgc
     5041 cggtaccgag tcaaacgcga acattacgtc cgagagttca accacagcca cacacaggaa
     5101 cagcggggtc gcatacagcg ccgctttttt accacggcca atggtgacat cgctgttttc
     5161 tggtttcgcc agttcagcat ccacttcctt ctggttaagt aggaaggcat gccctctgag
     5221 cttcggccag atagggaaga agcgtttaac catacggtaa gccagatgct gggagtaatc
     5281 ctcaatttca tcatcgtcat caccgctttt aagcatcatg accgccgtcc aggcaacgat
     5341 aatagcgaag acaacttcaa cgtacggccc cagactcagc aggctcgtac cgatggcgac
     5401 aaagatgccc ctgaagacga tggcaccaat gatcccccag tagagaacac ggtggcgata
     5461 acgatccgga acggcgaacc aggagaagat tgccatcatg acgaacaggt tatcgaccga
     5521 cagcactttc tccagcgcat aacccgtgac aaacagactg gcaacctcag caccgtggtg
     5581 gatatagagg aaaccggcaa atgccatcgc aaccacaacc cagaatacgg accagagcgc
     5641 agcactcttc agcgagattg gcttgtcatc acggtgcata aacaggtcaa taaagatggc
     5701 accgactgaa agcgcaataa aaacaatgac cgtttcagtc gggaagccga tgtgtgtgga
     5761 aaccatattt tacccttagg gatgtggatc agaggccaaa ttcagccggg tcgaagccca
     5821 acgcgatagc gatctcgcgg gaggccagtt tctcgtcctg atcgaagtta ccgtcacttt
     5881 tcgcaacggc aataccaaca cgtaaggcca gctgagcggc ctcaggctga tctttcagcg
     5941 ccaggatgta tttcatggtt tcgcccttgc caatttcaac atcgaaatcg aagcttgaaa
     6001 ccagtttatt gaagaactca atcacctcat tggtatcgaa gaccttcagc tcttctgaag
     6061 agcgcagaaa gcccatcatt ttctgctttt cttccgaact tacgccgtca ctcgatacgg
     6121 caatacgggc acatacagca acggtgccct gcatgaattt tttgtttttg aaacggccaa
     6181 cctggcgggt cagttcgtca cggcctgagt taatggcacc tttaactttg tcgaaaaagc
     6241 tcatacctgt ttcctttgtt gctgtgttat ttagaaccgg cgctccagcg aaatccccag
     6301 ccaaatgccc ggtccatttc gtcctggcct ttgaagtact ggttaattcg ctcgacttta
     6361 attgcgccgt tttcgtttac gagtctggca atggcgcaca atgtgcgacg gttctcacct
     6421 tcggtcaggc gggtctcaat gggcggttga tccggcacat gaatggtgac tacaccatca
     6481 gtcttgtccc agctgggaac accttcataa ataaaggcgt aaatcagcac ttcgcggata
     6541 tgcttccatt cacgtccatt gatatgcagc cactcgccgt ctgatacatc acccgtccgg
     6601 tcatcgccct tgagctgaac atacggttca tcgcgataat caccgaatgc attaccgagc
     6661 gcctgaatga ccgatttata cccgtcctga agctcaacaa aggcccccag atccaggtcg
     6721 attcctttgg agccgaacat acctgcaaag cctgatttgc tactgccccg gtgccagttc
     6781 aggttgatcc ggatttcacc gaagttatcc cgtttggtca ggctgattgc aggcttctct
     6841 tttgtcagcg ataccttgct caggctgatt tttgtctcaa ctggcggagg ggttacaacc
     6901 ggaggagttg gggctgcggg agccggttcg tcggcaatat ttacgccgaa atgctctgcc
     6961 agcggtttaa gtccaccgtt gaacccctgc gcaacgaagc ggaatttcca gtcattatta
     7021 cgacggtaaa attcccccag aattaaggct gcttcctgac ggccgctcag ttcgacactg
     7081 ccgctaacca ggccggtagc cccttgctcc acgtcgatgg aaagattacg aagaccggac
     7141 accgtctgcc caccgtcaca ggtcacggtg aacgcaattt tctggacatc aggtttcagc
     7201 cggttgagcg cgacagtaaa ggttgagtac tggccttcac tgaccaggct gacggtacca
     7261 tcatcgttac gtggctgacc ataaaacacc atgtccgcat caccctgcac cttcccgtcg
     7321 gcatacaggc gaaacgccga agcatccacc tggccgcccg aggtgatccg gacccgcaac
     7381 tcctgggctg gaacgggggc attgcccccc ggcgtcaggt tcattagcga ataaccgctg
     7441 aaacgatatc ggagtgcata tcgtcaattg tgcggccgcg acaggcatgg ccaagggcgg
     7501 taaagtccca gttgccgtta ttacggcgca gcgaagaaat aacgatcccg gtatgtgagc
     7561 cctgctcagt cagcttgtag cgagccagct ctttgccggt ttgatcgaca acgcggcaga
     7621 aagcgttttc gacgtcattg aacgactgac cacggaagct gtttaccgta aacgccaggt
     7681 attcaacgtt tgcaggcagg cgggagaggt tgaccttaat cacttcatcg tcaccgtcac
     7741 cctcaccggt caggttatcg ccactgtgca caacagcgcc gcaggtggat ttcagtttgc
     7801 ggaaccagat ggtgtcaatt gttttacctg tgctgtccat taatacacag ccagcatcga
     7861 gatcgattga gtcgttgcct ccaaacaatc ctccaagaag accttttttc ttaaccggat
     7921 cccaaccgag accgaaatga agctggctta atgcagatga ttctttgctg agtgataccg
     7981 tctggttctt aactaatgaa accatgtttt attccttaaa atggtaatga ccgag
//
LOCUS       DAZFDC010000172         7920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFDC010000172 DAZFDC010000000
VERSION     DAZFDC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.172"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..613
                     /locus_tag="ACHJ48_004294"
     CDS             <1..613
                     /locus_tag="ACHJ48_004294"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO4709724.1"
                     /translation="EALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERS
                     RNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALTDKFL
                     SLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFP
                     KKTCLAQYTQHELVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(621..887)
                     /locus_tag="ACHJ48_004295"
                     /pseudo
     CDS             complement(621..887)
                     /locus_tag="ACHJ48_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            1045..2229
                     /locus_tag="ACHJ48_004296"
     CDS             1045..2229
                     /locus_tag="ACHJ48_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO4709725.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNLKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDIEDNSTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            2229..2741
                     /locus_tag="ACHJ48_004297"
     CDS             2229..2741
                     /locus_tag="ACHJ48_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO4709726.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDDESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            2796..3161
                     /locus_tag="ACHJ48_004298"
     CDS             2796..3161
                     /locus_tag="ACHJ48_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4709727.1"
                     /translation="MKETKNIDTENTVVTDTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            3170..3325
                     /locus_tag="ACHJ48_004299"
     CDS             3170..3325
                     /locus_tag="ACHJ48_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO4709728.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3312..6115
                     /locus_tag="ACHJ48_004300"
                     /pseudo
     CDS             3312..6115
                     /locus_tag="ACHJ48_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            6128..6616
                     /locus_tag="ACHJ48_004301"
     CDS             6128..6616
                     /locus_tag="ACHJ48_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4709729.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
     gene            6773..7345
                     /locus_tag="ACHJ48_004302"
     CDS             6773..7345
                     /locus_tag="ACHJ48_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO4709730.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKHEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDMPSSRVKIGSNVDIGANCCIIGLSRKIGDN
                     VKIGAMSFINKDIPSNCTYITKKSGVVLYK"
     gene            complement(7389..7919)
                     /locus_tag="ACHJ48_004303"
     CDS             complement(7389..7919)
                     /locus_tag="ACHJ48_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4709731.1"
                     /translation="MLAGTNEFIGEGDAYIPPHTGLPANSTDIAPPDIPAGFVAVFNS
                     NEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSPDGECQKWNGTSWAKDA
                     EAEKLFRVREAEETKNSLMQVASEHIAPLQDAVDLDIATEEEASLLAAWKTYRVLLNR
                     VDTTVAADVEWPVAPQ"
BASE COUNT         1981 a         1871 c         2191 g         1877 t
ORIGIN      
        1 tgaagcgcta caccacctga atatacagca tctgcgacgg tcgcatagcc ttcgccatgg
       61 caggcgtcat acccgcaaag gcgaaagagg tacgattaac atagtgaacg gaacaccaat
      121 tcacgaacgt tcccgaaata tcgataacag acgctctcta gggcattggg agggcgattt
      181 agtctcaggt acaaaaaact ctcatatagc cacacttgta gaccgaaaat cacgttatac
      241 gatcatcctc agactcaggg gcaaagattc tgtctcagta aatcaggctc ttaccgacaa
      301 attcctgagt ttaccgtcag aactcagaaa atcactgaca tgggacagag gaatggaact
      361 ggccagacat ctagaattta ctgtcagcac cggcgttaaa gtttacttct gcgatcctca
      421 gagtccttgg cagcggggaa caaatgagaa cacaaatggg ctaattcggc agtactttcc
      481 taaaaagaca tgtcttgccc aatatactca acatgaactg gttgctgctc agctaaacaa
      541 cagaccgaga aagacactga agttcaaaac accgaaagag ataattgaaa ggggtgttgc
      601 attgacagat tgaatctaca agtgcctttt tctgtcagcg gttctccttt ccagccaatg
      661 agaaccgtca ggcgcgcgcc ccgtggcggt agctgcaact gaccatctgc atcatccagc
      721 gtgatggtga gctggtccgc ctcaaatccc cggttgtcgg tcagtgacag gctcatcagg
      781 cgctctgcca cgcctgacag cgttttaccc tccgcgagaa tatcaaaatc cggcactttc
      841 acgtggtctg tgccctgact gagcaattgc atggtggtgt cggtcatctg ttccctccct
      901 gtgcggcatg gtcgcatgtg cgtgcggagg gggttactgc tttttgttgt cgccgtggcg
      961 ggagaacggc gcaggggtga gattacgcgc gtggtgggtg atgattgttg ccgaatcatt
     1021 taacggatac aaggggctga agctatgagt gaaactcgtt ttcatggtgc ccgtgttacg
     1081 gaaagtaccg acctggtaac agcgattaat gatgttgatt ccagtgttat cggtatcgtg
     1141 gcaacggcag atgatgcgga cgcggagctg ttcccgctga acaagcccac actgctgacc
     1201 cgcgtcaatg acgtgctggg aaaatgcggg actacgggga cgctttatcg tgcgcttaag
     1261 gctatcgcag accaggtgag cacaaaagtg atcgtcgttc gcgtggctga acacaaagaa
     1321 gaaggcggta agacgcagga tcaactggtt atcggtggtt ctgaatctga cggcagctat
     1381 acggggatgt atgcgctgct tgttgcagag caggatgaaa gcatcggata ccgtccgcgt
     1441 attctggccg cgccggagct ggacacggag gcggtaacaa aatccctgtg cgtgattgcg
     1501 ggtaaactgc gcgcgtttgt gtatgccaca tgtcatggtt gtaacacgat ggctgaggcg
     1561 attacctacc gccagaaatt caacgaacgt gaggtgatgc tcttatggcc tgacttcatc
     1621 gcctacaacc tgaaaagtgg caaaaacgaa acgttccccg cgcctgctta tgcgtgcggc
     1681 cttcgtgcgt acattgacca tgagcagggc tggcacaaat cgctgtccaa cgttccggtt
     1741 aaaaatgtgc ttgggatgtc gaggcatgtg ttctggtcgt tgcaggccga agacagtgat
     1801 gccaacagcc tcaacaacaa agaaatcacg accattattc gtcgcaacgg gttccgcttc
     1861 tggggcaacc gcacaccgga aacgaacgcc tacatctttg aggtgtatac ccgaaccgca
     1921 caggtgctgg ctgattcaat tgcggaagcg cagtttgaaa ccatcgacag tccactgaca
     1981 cctgcgaacg tgaaggatgt tatcagtgcc atcagggcaa aactggattc actggttact
     2041 gccgggaaac tgattggcgc ggagtgctgg tatgacatcg aggataacag caccacgaat
     2101 ttacgtcagg ggcgtgtgcg tattcgctac aaatatacgc ccgttcctcc gctggaagac
     2161 atggagcttt accagtcgtt tactgatgaa ttctttggtc ccgcatttgc ggtgctggga
     2221 ggtgcctgat ggctgtacca aaacatcttc gcttttttac gctgtttgtg gatggtgaaa
     2281 acgaagtggg taaggtgacg tccgtcaccc tgcctaaact gacgcgcaaa accgacagct
     2341 accggggtgg tggcatgatg ggtgcggtaa gtattgacct cggcctggac gactccgcgc
     2401 ttgatgcgag ctttgtcatg gggggcgcag ttcgtgagct gttcctgaag tatggcggca
     2461 cgattgacgg cacgctactg cgttttgcgg gggaatacta caccgatgat gaaagcgacc
     2521 tgtatgaagt cgaaatgcgc ggacgtgtga cggaaattga tatgggggaa gccaaacagg
     2581 gcgaagccac atcacacact tacgccatta aaaacaccta ctacaagctg agtgttaacg
     2641 atcgcccgtt gtgggagatt gacctgctga acttcattta ccggaaggac ggcaaggaca
     2701 ttgtgcccga tcgcatccgt tccgcgcttg ggcttggctg ataagtaata tgcaggcggc
     2761 gcagtgcgtc gcctctgact gaaaggagtt tcctgatgaa agagacgaaa aacatcgata
     2821 ccgaaaacac ggtagttact gacactgtga aagaaaccag tgagcgtggt gtaaaactta
     2881 cccaaccaat tgagcgaggc ggcgaaaaaa tcacgtatgt ggagatcacc ggggctattg
     2941 agcaggctgg atctctgcga gatttgtcgc tgtctgatgt gctgaatctg aaagcggaat
     3001 ccatgtttac gctgctgtca cgcgtgacat caccgcgact ggatgaagtg acgatcaaaa
     3061 aaatggcatc ccgtgacttt attcagttat gtgtggttgc cgtaaatttt ttgagcggtg
     3121 cggactctgg cgggaagaac gaacaggcga cggaagcctg atcacggttg tgtgctttga
     3181 gcacatagaa gactttgtgg cagatattgc cgttattttt aactggtcgc ccgccgaaat
     3241 cttcatgatg acgcccggcg aagtggttag ctggcgtgag cgggcggcac ttcgcagcgg
     3301 gaatgcagac aatgaagact cttgatatcc gggtcgcttt cagcgccgtt gacaggctga
     3361 cccgacctgc cgaaaacgca cgccgcctga tggggcagtt tggtgactcc atccagcgaa
     3421 cgcagggggc gatcaaaaat ctcgagcgtc aggcgcgatc atttgagcgc gcccgcgacg
     3481 ctgtcagtaa agcggatgct ggcatcgtga aagcacgacg ccagcttaac gcccttaatc
     3541 agttacaacg cacgggtaca gtgctcagcg aaaaacaaca aaagctgatg cagcagttaa
     3601 gcacccggct tgaacgcctg aatgaatcgc gcacacggga aattcagaaa atgagggaac
     3661 ttggcggaga gctgaaacgc cacggcattt ccctgacagg cagcgataac accattcagc
     3721 aggccatcag acgcaccgaa caatacaaca accagcttga acgcgaacgg caggcgcttg
     3781 cgcgtgtaac gcaggcgcgt gagcggtatt cgcacgcgca ggaaaccgcg ggaaaactga
     3841 aaacaggtgg tgcgctggca attggtgcgg cagcggcggg cggctatgct gccgggcgtt
     3901 ttttgcagcc tgcgatcggg ttcggcaaag agatgtcccg cgttcaggca ctgacgcgaa
     3961 tcgacaaaaa cagcccgcag tttaaggcgc tgcgtgagca ggcgttaaaa cttgctgaaa
     4021 cacagtttac tgcgagtgat gccgccagtg ggcagagctt tctggcaatg gctggtttta
     4081 ctccgcaggc cattcaggcc gcattgcccg gtgttcttaa tatggcgctg gcaggtggcg
     4141 tcgaactcgg cgagacggct gatataggct ccaatatcct cacacagttc aacctgacag
     4201 ccgatcaaat ggaccgggtg ggcgatacgc tgacagcagc atttacccgg accaatactg
     4261 atttacgcgc gctgggcgaa accatgaagt ataccggtcc ggttgccgca aaacttggta
     4321 tcagtcttga agaagcggcg gccatggccg ggatgcttgc caataatggt cttcgcggaa
     4381 gcgatgctgg cacggccatg cgcgcaagtc tgtcccgcct tgcatcaccg ccaaaagctg
     4441 cggctgatgc actgaaagag ctgggggtgt cagttgctga cgccagaggc aaaatgcgcc
     4501 cgatggagga tgtgctgctt gatctctata aggcgacaca aaaatacgga caggtggacc
     4561 aggtctcctt cttcaaggac atcgccggag aagaggcgtt cgttggtttg cagacgcttg
     4621 ttgcggcggc tggttcagga gagctgcaaa aactgaccag agaattgcag ggggcaaggg
     4681 gagaggccga tcgcgtcgca aaagtaatgg ctgataatct tgatggggac ctgaaaaatc
     4741 tcgacagcgc atgggaaggt cttcgtattc gcatcagtga tctggttgac ggtccgctgc
     4801 gttctgtcac gcagtggctc acgcgggtgc ttgaaaaaat cacctcgctg gcgcaggccc
     4861 atccggtact gacgcgccag ctactgatag caggcggtgc gttgctggca atgactgcaa
     4921 cgattggctc gttgtcgctg gttattgggg tgctttacgg gaagctggcc accctgcgtc
     4981 ttggttttga cattcttacc cggtcaatga atgtcgtcag ggtgttacct gcgctatggg
     5041 gaatggtgac gggttccgtt tctttactgg gaggcgttat cggggcgttg ttcagtccgg
     5101 ttggtcttat cgtggctgcg cttgccggag ctgccgttct tatctggaaa tactgggatc
     5161 ctatcagggc tttctttgcc ggggtgttca gcgggattat ggaaaggctg accccgttgc
     5221 gcgacacctt tgaacggttt ggccctgttt ttgacgtaat cggaagcggt tcagccaggt
     5281 gtttaactgg tttaaatcgc tgctgtcacc gatggagtcc agcaaggaaa cgctggataa
     5341 atgtaccagt gctggcgagg tattcggtaa cgttcttggt ggtgcgttac agcttgttct
     5401 gacgcctgca aaaatgttgc tggatacgct ggcgtggata cttgaaaaac tcggtgtgct
     5461 tccggatgaa gcggaaaggg cgcgcaagaa aatcgaagac gcacaacgtg cggccattct
     5521 tcaggacaag gttgctctgt ttcagggaga cattgcgaaa atcaatccgc cgaagtctgc
     5581 gggaaatagc aatggcaccg gagatgataa acccaaagac aacaaacctc tcacagacag
     5641 caataccggc acgctacgca gactcagcaa aattgctgat aacacaggta agctggttga
     5701 tgagacaaaa aaacgcattg gccccggcga tattgtcttt aagaacctgc cccgcgcact
     5761 tgctgttcgt ggggagtggc aggagcggaa gattgcgcag gtcagtaagc ctgcccccgc
     5821 aattaatatc acacccgtgg tcccggctcc gctgcctccg gcgctggtcc ctgttgttgc
     5881 ggccagctcc cgcccggtgg tggaggccat acgatcgcca gtggcatcag ttcctgcaac
     5941 ttcccgtaac cgggagcctg ttgcctccgg atttggcggt gaaattcatg ttcatctgca
     6001 taacgttgtt acgcagaatc cccgcgaact ggcgaaactg gtcggcgaaa tggtcagggc
     6061 agaaatggaa cggcgcgccc gtgccaggcg tggcagtttt tacgataaag attgaggagt
     6121 catggccatg atgatgatct acggcatgtt tgtttttgag ctgcgcacgt tgccgcatca
     6181 gcagttacag caaaacaaaa gctggcgaca tgtgaaaaat gaacgcgtta accgttcagc
     6241 aagctggcag tatatcggtg caggtgatga tcgcatcgtt ctttctggtg ttctttatcc
     6301 tgagattact ggtggcgaag tgtcgctgtc gttgttgacc acgcaggcat atacaggacg
     6361 tccttggcct ttgattgatg gcgtcgggca gatttacggc atgtatgtcc tgaccggaac
     6421 gaatacgacc cgctccgagt ttgatcgcta cggtaaggcg aaaaagatag aattttcact
     6481 gactcttgaa cgctgtgatg aggatttgcg ggagcgcctg caatcctcat cgttcagcga
     6541 tatgctgtcc ggcttcaaag ataaggtgac atcatccctt aatagcgcgg ccagctcagt
     6601 taaggggctg ctctgattta acgtatgtcg ccaatttcct gatgaaggtg actggcgact
     6661 tgctgttgta tgtccttctc agaaaattgt ttttgaataa caaataacag gattttataa
     6721 tctcttaacc ttataacatg tgtggtctga aataataatt aaggagatta tcgtgctatc
     6781 ttacttaatg gcaattcact ttgttttatt tggaaactct actaatttaa aaaacttctg
     6841 gaaacatgaa gtaattcggc ggaaacgtat ggatatctgg aggcttttaa gagagaaaaa
     6901 gcagcgtaac cggaatttcc ttttctggtg gcggttggct aacgaaatgt atattaatgg
     6961 taataaatta cataagaaag cagccaaaaa gttaaacagt aaaataatta acaagtttgg
     7021 ttgtgaaata ggattgggcg caaatattgg aaaagggttg acaattcccc atcatgctgg
     7081 aattgttgtt cattttgctg ttgatgcggg tgaaaatctg gtattacgac agaatactac
     7141 cattggacag atagatggtg atatgcctag ttcaagagta aaaattggta gtaacgttga
     7201 tatcggagct aattgttgca tcattggatt atcacgtaaa attggggata atgtaaaaat
     7261 aggtgcaatg tcttttataa ataaagatat accatcaaac tgcacatata taactaaaaa
     7321 gagtggtgtt gtattgtata aatagagtac ataaagccat cgatatttct gtcgatggct
     7381 ttttcttttt attgtggggc gactggccac tcaacatccg ctgctactgt tgtatcaaca
     7441 cgattcaaca atacccgata tgtcttccat gcagccagta acgatgcctc ttcctccgtc
     7501 gcaatatcca aatctacggc atcctgaagt ggcgcaatat gctcactggc tacctgcatc
     7561 aggctgtttt ttgtttcttc tgcctcccgt acccgaaaca gtttttctgc ttctgcatct
     7621 ttcgcccagg atgtgccgtt ccacttctga cactctccat ccggcgacaa ccaggtaaca
     7681 ttttccggta acggaccgag ttcagaaata aataacgcgt cgccggaagc cacgtcataa
     7741 accgttttac cccgatgatc ttcaacgaga tgccacgatg cctcattact gttgaaaaca
     7801 gccacgaagc cagccggaat atctggcggt gcaatatcgg tactgtttgc tggcagacct
     7861 gtatgaggcg gaatgtatgc gtcaccttca ccaataaatt cattagttcc ggccagcaga
//
LOCUS       DAZFDC010000173         7759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFDC010000173 DAZFDC010000000
VERSION     DAZFDC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.173"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(271..414)
                     /gene="ypaB"
                     /locus_tag="ACHJ48_004304"
     CDS             complement(271..414)
                     /gene="ypaB"
                     /locus_tag="ACHJ48_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO4709732.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVINNCS
                     AIS"
     gene            542..2827
                     /gene="nrdA"
                     /locus_tag="ACHJ48_004305"
     CDS             542..2827
                     /gene="nrdA"
                     /locus_tag="ACHJ48_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO4709733.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3054..4184
                     /gene="nrdB"
                     /locus_tag="ACHJ48_004306"
     CDS             3054..4184
                     /gene="nrdB"
                     /locus_tag="ACHJ48_004306"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO4709734.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4184..4438
                     /gene="yfaE"
                     /locus_tag="ACHJ48_004307"
     CDS             4184..4438
                     /gene="yfaE"
                     /locus_tag="ACHJ48_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO4709735.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4492..5142)
                     /gene="inaA"
                     /locus_tag="ACHJ48_004308"
     CDS             complement(4492..5142)
                     /gene="inaA"
                     /locus_tag="ACHJ48_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO4709736.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5223..6413)
                     /locus_tag="ACHJ48_004309"
     CDS             complement(5223..6413)
                     /locus_tag="ACHJ48_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO4709737.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGGSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6565..7443
                     /locus_tag="ACHJ48_004310"
     CDS             6565..7443
                     /locus_tag="ACHJ48_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO4709738.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVVSGKI"
BASE COUNT         2088 a         2005 c         1825 g         1841 t
ORIGIN      
        1 cttaagggat accagttata tttaaactaa attaaagtaa tgaataattt tcttataata
       61 taaggcaaat taacaaaatg gcttcgcatt taacaataac cgaatagaaa acaaccattt
      121 cgacatcaac aatcgcttac attcgcttat atattgacca caactgatac atcagattat
      181 gtgatgactc gtgcttagat caatttttgc aatcattagc aaaaagatta ataaaccatc
      241 tatatcaatt tatccaacct attctgccgt tcaagaaatc gccgaacagt tatttataac
      301 aaatttttct cttcccattg actttcccgg acaccttgtc tgacctaagg tgcgcgaaag
      361 ccactttttc cttcctgagt tatccacaaa gttatgcact tgcaagaggg ccattttcac
      421 actatcttgc agtgaatccc aaacataccc cctatatata gtgttctaag cagcttcccg
      481 tactacaggt agtctgcacg aaactattgc ggaaagaatt ccaaaaacag gtacgacata
      541 catgaatcag aatctgctgg tgacaaagcg cgacggtagc acagagcgca tcaatctcga
      601 caaaatccat cgcgttctgg attgggcggc agaaggactg cataacgttt cgatttccca
      661 ggtcgagctg cgctcccaca ttcagtttta tgacggtatc aagacctctg acatccacga
      721 aaccattatc aaggctgccg cagacctgat ctcccgtgat gcgccggatt atcagtatct
      781 cgccgcgcgc ctggcgatct tccacctgcg taaaaaagcc tacggccagt ttgagccgcc
      841 tgcgctgtac gaccacgtgg tgaaaatggt cgagatgggc aaatacgata atcatctgct
      901 ggaagactac acggaagaag agttcaagca gatggacacg tttatcgatc acgaccgtga
      961 tatgaccttc tcttatgctg ccgttaagca actggaaggc aaatatctgg tacagaaccg
     1021 tgtgaccggc gaaatctacg aaagcgccca gttcctttat attctggttg ctgcgtgcct
     1081 gttctcgaac tacccgcgtg aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt
     1141 ttccacattt aaaatttcgc tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg
     1201 tcagttcagc tcctgcgtac tgatcgagtg cggcgacagc ctggattcca tcaacgccac
     1261 ctccagcgcg attgtgaaat atgtttccca gcgtgccggg atcggcatca acgccgggcg
     1321 tattcgtgcg ctgggtagcc cgattcgcgg tggcgaagcg ttccataccg gctgcattcc
     1381 gttctacaaa catttccaga ctgcggtgaa atcctgctct cagggcggcg tgcgcggcgg
     1441 tgcggcaacg ctgttctacc cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa
     1501 aaacaaccgt ggtgtggaag gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa
     1561 caaactgatg tatacccgcc tgctgaaagg tgaagatatc accctgttca gcccgtccga
     1621 cgtaccgggg ctgtacgacg cattcttcgc cgatcaggaa gagtttgaac gcctgtatac
     1681 caaatatgag aaagacgaca gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc
     1741 gctgatgatg caggaacgtg cgtctaccgg tcgtatctat attcagaacg ttgaccactg
     1801 caatacccat agcccgtttg atccggccat cgcgccagtg cgtcagtcta acctgtgcct
     1861 ggagatagcc ctgccgacca aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc
     1921 gctgtgtacg ctgtctgctt tcaacctggg cgcaattaat agcctggatg aactggaaga
     1981 gctggcaatt ctggcggttc gtgcacttga cgcgctgctg gattatcagg attacccgat
     2041 cccggccgcc aaacgtggag cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt
     2101 cgcttactac ctggcgaagc atggtaaacg ctactccgac ggcagcgcca acaacctgac
     2161 gcataaaacc ttcgaagcga ttcagtatta cctgctgaaa gcgtctaatg agctggcgaa
     2221 agagcaaggc gcgtgcccgt ggtttaacga aaccacttac gcgaaaggga tcctgccgat
     2281 cgatacctat aagaaagatc tggatgccat cgctaatgaa ccgctgcatt acgactggga
     2341 agctctgcgt gagtcaatca aaacacacgg cctgcgtaac tccacgcttt ctgctctgat
     2401 gccgtccgag acttcttcgc agatctctaa cgccactaac ggtattgaac cgccgcgcgg
     2461 ttacgtcagc atcaaagcgt cgaaagacgg tattctgcgc caggtggtgc cggactacga
     2521 gcatctgcac gacgcctatg agctgctgtg ggaaatgccg ggtaacgatg gctacctgca
     2581 actggtgggt atcatgcaga aatttatcga tcagtcgatc tctgctaaca ccaactacga
     2641 tccgtcacgc ttcccgtcag gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac
     2701 cgcctacaaa ttcggggtca aaacgctgta ttatcagaac acccgtgacg gcgctgaaga
     2761 cgcgcaggac gatctggtgc cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa
     2821 gatctgatat tgagatgccg gatgcggcgt aaacgcctta tccggcctac ggctcggttt
     2881 gtaggcctga taagacgcgc cagcgtcgca tcaggcacag gatgcggcgt gaacgcctaa
     2941 tccggcctac ggctcggatt tgtaggcctg ataagacgcg ccagcgtcgc atcaggcaca
     3001 ggatgcggcg taaaatgcct tatccggcat taaactccca acaggacaca ctcatggcat
     3061 ataccacctt ttcacagacg aaaaatgatc agctcaaaga accgatgttc tttggtcagc
     3121 cggtcaacgt ggcccgttac gatcagcaaa aatatgacat cttcgaaaag ctgatcgaaa
     3181 agcagctctc tttcttctgg cgtccggaag aagttgacgt ttctcgtgac cgtatcgatt
     3241 accaggcgct gcctgaacac gaaaaacaca tctttatcag caacctgaaa tatcagacgc
     3301 tgctggactc cattcagggt cgtagcccga acgtggcact gctgccgctg atctcgatcc
     3361 cggaactgga aacctgggta gaaacctggg cgttctcaga aacgattcat tcccgttcct
     3421 atactcatat cattcgtaat atcgttaacg atccgtctgt tgtgtttgac gatatcgtca
     3481 ccaacgagca gatccagaaa cgtgcggaag ggatctccag ctactacgat gagctgatcg
     3541 aaatgaccag ctactggcac ctgctgggcg aaggcaccca cactgttaac ggtaaaaccg
     3601 tgaccgttag cctgcgcgag ctgaagaaaa aactgtatct ctgcctgatg agcgttaacg
     3661 cgctggaagc gatccgcttc tacgtcagct ttgcttgttc ctttgccttt gcagaacgcg
     3721 aactgatgga aggtaacgcc aaaattattc gcctgattgc ccgcgacgaa gccctgcacc
     3781 tgaccggtac tcagcatatg ctgaatctgc tgcgcagcgg tgcggatgat ccggagatgg
     3841 cggaaattgc cgaagaatgt aagcaggagt gctatgacct gtttgtccag gcggcacagc
     3901 aagagaaaga ctgggcggat tatctgttcc gtgacggttc gatgattggt ctgaataaag
     3961 acattctctg ccagtacgtt gaatacatca ccaatatccg catgcaggca gtcggtttgg
     4021 atctgccgtt ccagacgcgc tccaacccga tcccgtggat caacacctgg ctggtgtctg
     4081 ataacgtgca ggttgctccg caggaagtgg aagtcagttc ttatctggtc gggcagattg
     4141 actcggaagt ggacaccgac gatttgagta acttccagct ctgatggctc gcgttaccct
     4201 gcgcatcact ggcacacaac tgctgtgcca ggatgaacac ccttcccttc tggcggcgct
     4261 ggaatcccac aatgtggcgg ttgagtacca gtgccgcgaa ggttactgcg gctcctgtcg
     4321 cacacgcctg gttgcaggcc aggtggactg gattgccgaa ccgttagcct ttattcagcc
     4381 gggggaaatt ttgccctgtt gttgccgagc aaaaggcgat attgaaatcg agttgtgatc
     4441 gggtactagc gccagatacg acgcttacgc gtattatctg gcctacaacg attacatggc
     4501 gtagtaatac gcttttacct gctcccagtc cgctttgggg atcggctcca gatatttttc
     4561 cagctggcgg aagtcatgat taatcgcttt atcgcgacgc aagcggcgac gacttttctc
     4621 cagatcaaga aaaccagctt ctgccttacc ttctgttttc acatagatat ggcgaacata
     4681 acaacagcca tgctgacgat taacgctatg cattttctta aacgccagcg ccactgcttt
     4741 caacatggct tgccgtactt cgtcagaata gggcgttact gcatgctggg cataccagtc
     4801 agcaatgctg atgaaccccg ccatatcttc agtcaccagc aacgcgcgcc attccccctc
     4861 aattttcacc gcttcaccaa aaacgatctt cggcacgatg acaccggccc gttccagttc
     4921 tttaattacc gcaacctcac ggacaatcgt tggtcggccg aaaggataac gtacggaatg
     4981 aaacagatga tgcgtcatgc gctttacata tagctttttg ccgttgcgct cgacgcattg
     5041 caccccgctc ataccattac ggcgatagtt aggctcttca acccagtcgc cctctgttgc
     5101 ccaccagtga ttaaattcgt cgtgctttgc tgaaactgcc ataccctatc gcctgtcatt
     5161 ttttattaac gacaatgact ataggtggtt acctgcgaaa aatcttaatg aaacgtgtcg
     5221 tattaatgaa tttcttttgc cactactagc gcgacaccgc tttttccctt catttgcagc
     5281 gcatagactc caccagccac taccactaat agtgcgccca gcagataaat cattcggtag
     5341 ccagtatgtg caaccatcat ccccatcaca caggctccca gcgccaggcc tgcatccatc
     5401 gcattgaaga agagggagtt tgcgacaccg attttatgcg gttccactga actgataatc
     5461 tgcgtctgaa aaactggcgt cacggaacca tacccgatac caatcaacgc gccagaaacg
     5521 accatcatta cgctgccctg ggtgtagccg agaaccacca gcccaaccgt aaagaacagc
     5581 aggcaggggt aaaccacata tttcgggcct tttttgtcgc aaacattgcc ggtgaaggta
     5641 cggcagatca tcaggaagat ggcgtagcag agcaggaaat tactggccgc cgacatcaga
     5701 ttaagttcgc gggcgtaaag cgccagaaag gcagaacccc cggcatacga aaaggtcata
     5761 aagaatgtaa ccatggcaaa cggcaatgcc gcgcgatcga acatggcggc gaagcccatc
     5821 tttggctttg aaccgtcggc atggctgata accggcggca cggtcataat cagcgacagg
     5881 ataatcccca ccgccgcaac gccagtacat aaccagaaag ccatattaaa ggcctcccag
     5941 cgcgccatat tcaggccaat ccacggcccg accaccattg ccagccccat tgccagagag
     6001 aaaaaactga tgccctcccc acgtcgggag gccgggatca gccgcgcgga aatcgttccc
     6061 tttaccgttg tgataacccc gaaggtaata ccatgaagca ccctaataaa aagcagtgat
     6121 tctattgagt ggcaaaaagg atacagcgca gtgaccacca aaaaggccag agaagagagc
     6181 accagaatag ttttattcga atatttaccc acccattgcc cggcaaaggg tcgaataaca
     6241 atcgccgcaa ttaaaaataa agtcaccaac aaacctgctt tatcaggaga ggcatgcaag
     6301 cggtcagaga tataaattgg caacaatgtt agtaaaacat aaaagacgaa gaaaataata
     6361 aagctgatga tggttattgc ccaaaaatcc ttcgtccata acttctcttt catactctac
     6421 cgcctgtcag gatgacgcac gcagacaaca ctcccgttac tgcggttgcg tcaaattgtg
     6481 atgtactttg cagaacagaa gtataaatgg cggcatgcta ttagagaaat taatcgtttt
     6541 aatattattg attagcaaac tctaatgact cttacccaaa taaattcttt actcgccgtg
     6601 ctggattacg gtggctttac tgaagccagc aagcgattgt tcatgacgca gtcggcggta
     6661 agtcaggcaa ttgctgccct cgaacaagaa cttggtgtga atatactgtt acgcgatcgc
     6721 cgtaaagcta tacaactgac cacagcaggt caccgcattg tgcaacatct gcgcgccatt
     6781 aatcgggaag ttaacgcagt aaaagagata gccgagcagg aaaagcagaa ccctcaaaga
     6841 acgctgagac ttggttgttt ccccagcgtc tgcgcctgta tcttaccggt tgtgattcgc
     6901 tactttgaaa ttcatcatcc gaatataaaa attattcctt ttgaggaaaa tagcacagcg
     6961 attattgatt cgcttcagaa tgaatccatt gatgccggtt ttgttcattt tccggtgagc
     7021 ggaatgtact ccgtgcccat ttatcaggat aaattcaccg tagtgttacc gcccaaccat
     7081 gctctggcaa aaaacagcac tattacagtg gaagaattaa tgggtgaacc attaatcatt
     7141 agtaaaggac gctatgaatt aagcattatg gcactattta aagaaaagaa tattacccca
     7201 cagataaaat atgaatttaa tcacccggat acagcgatca gttttattcg acagggatta
     7261 ggcatcgcct tattaccaga gttaacgctg aaaaccattg cagatgaatt gtgttccgtt
     7321 cctctcgaac caaccttcta tcgacagatt tcgttgctgg ctaaagaaaa gccggtagaa
     7381 ggcagtccac tgtttttact acaaatgtgt acggaacaac tagtggtgag tggaaaaatt
     7441 tgatgtaaga catgagattg atgacaaatg caagactgcc tgattcgcca cacttagttg
     7501 tactatagcc tttaccaggc gtaataattc gttttcacct gattttggcc cactttgtga
     7561 attggcgata tatttatcca gttgggagaa gtgacaatga ctccaggtag aaaccggtat
     7621 gaagcgcccg actcactgtc aaaaccgatt gttgagagta atgacactcc ggcgaacagt
     7681 acacagttta ctctggagcc attctcaatc ttcaagtggg atacctaata acttgtcagg
     7741 gaagttatca atatctgtt
//
LOCUS       DAZFDC010000174         7744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFDC010000174 DAZFDC010000000
VERSION     DAZFDC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.174"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..230
                     /locus_tag="ACHJ48_004311"
     CDS             <1..230
                     /locus_tag="ACHJ48_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709739.1"
                     /translation="TRYEWDIYYLYFKIDNKRILHTLLKKDVKYISHYKISVFNKFEI
                     ACDSDGFYIFKALIQLRRFTGFKNVRLYQLH"
     gene            273..833
                     /locus_tag="ACHJ48_004312"
     CDS             273..833
                     /locus_tag="ACHJ48_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4709740.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLMPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     STGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            919..1620
                     /locus_tag="ACHJ48_004313"
     CDS             919..1620
                     /locus_tag="ACHJ48_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4709741.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKVPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1673..4195
                     /locus_tag="ACHJ48_004314"
     CDS             1673..4195
                     /locus_tag="ACHJ48_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4709742.1"
                     /translation="MNKSVVSISAAMLVLLCQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDLTWQHGMTNTLTFNSG
                     SRIADGYQALMLGGVYGSSLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERHAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGITFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSERQVAP
                     VAGAAVKVIFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            4211..5545
                     /locus_tag="ACHJ48_004315"
     CDS             4211..5545
                     /locus_tag="ACHJ48_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4709743.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWANCTNTQIGQTEDGRTALI
                     EFGKINMTDTYFAPAGSLLATTVVPPTNYTSGGATGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFYDAGGPDGLSDVYATWFAFVGLKQTMAGVTLGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTSATTSWCGNNNTDSSGVGFAKPSGTIYNCVQPNAY
                     IQLSGTSGILFGHDEPGEDSSVHWDFWGADNGFGYGMRSANRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTGNFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAHPDTAMTLVGQPGIAKLTPGGNAAGW
                     YPVFEGATLEGATHPGYSSYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5542..6231
                     /locus_tag="ACHJ48_004316"
     CDS             5542..6231
                     /locus_tag="ACHJ48_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4709744.1"
                     /translation="MKSVLLLLTILFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLGNDGSSS
                     YIELNNPTPLNISFTSISIGATKVQQESDMMIKPFSQRRYWLASNNVSGVVAATYLDD
                     NGAQIKQQLAQ"
     gene            complement(6377..7159)
                     /locus_tag="ACHJ48_004317"
     CDS             complement(6377..7159)
                     /locus_tag="ACHJ48_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO4709745.1"
                     /translation="MSFSIEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANL
                     LLVDIKEPDNRYLKHLTHEGVEVEFMTIDITKPDASCTIINRCLERFGQLDILVNNAG
                     VCNINRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGR
                     WSPGYAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKG
                     RIPAQRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         2153 a         1843 c         1762 g         1986 t
ORIGIN      
        1 tgacaaggta tgagtgggat atctattact tatattttaa gatagataac aaaagaatat
       61 tgcacaccct gctaaaaaag gatgtcaagt atattagtca ctataaaata tcagttttta
      121 acaagtttga aattgcttgt gatagtgatg gcttttacat tttcaaggca cttatacagt
      181 taaggagatt taccggtttt aaaaacgtca gattgtatca attgcattaa ttaactcgtt
      241 ctatattaac tctaaatttc aaggatatta atatgttttc tactttcaaa aaagcagctc
      301 tgctggcagc tattgcatta cctttttcaa ctatggctgc gcctacagtc acttttcagg
      361 gtgaagtaac cgatcagacc tgttccgtaa atatcaacgg tcaaaccaat tcagtagtat
      421 tgatgccgac cgtagccatg gctgacttcg gtgcaacttt agctgatggt cagagcgcag
      481 gccagacgcc gtttacggtt tctgtgtcta actgccaggc tccaactggt gcagatcagg
      541 caatcaacac caccttcctg ggctacgacg ttgacgctag cacgggtgtt atgggaaacc
      601 gtgataccag cagcgatgcg gcgaaaggct ttggcattca gttaatggat tccagcactt
      661 ctggtaaccc agtaactctg gctggcgcga ctaacgtacc gggtctgacc ctgaaagttg
      721 gcgataccga agccagctac gacttcggtg cgcgttactt cgttatcgat agcgctgctg
      781 ccactgccgg taaaattacc gctgtcgcag aatacaccct gagctacctc taattaatct
      841 actggctggc agtaaatgcc agccagtatt tcttcgcaca attaatagtg atttttcatg
      901 gacatggaaa agataagaat gaacattttt gcatatttac tggtacttgt attttccatg
      961 agcatgagca gcagcgcgtt tgccagcgtg gtaatgaccg gaacccgtat tattttccct
     1021 ggtgacgcaa aggaaaaaac catccagttg cgaaatacca gcgatcagcc ctatatcatt
     1081 aatatccatg ttgaggatga acgtggttct gacaagaatg taccgtttat gccaaccccg
     1141 cagacatttc gcatggaagc tgccgcaggt caggcgttac gcctgctcta cactggtaat
     1201 aatttaccgc aggatcgcga gtctgttttc tggtttagtt tcagtcaact accttatctg
     1261 aataagaatg ataaaagtca gaaccagctc atcctggccc tgactaatcg agtcaaaatt
     1321 ttctatcgtc ccagctcgat tgtcggtaaa tccagtgacg cacccaaaaa cctgacttac
     1381 caggtaaaac agaaccgcat tgaagtgacg aatcccacgg gctattacgt cacaattcgc
     1441 gccgctgaac tgcttaataa tggtaaaaaa gtccccctcg cgaattcggt aatgattgct
     1501 cctcaaagca caactgaatg gacactaccc tctggcatca gtgtcgctcc cggtgcgcag
     1561 atccatttag tgaccgtcaa cgactatggc gtaaatgtta cgtctgagca tgccttataa
     1621 cctctttctc cttttttgag cggtatcctt tttcacgcac gagtacgtcg agatgaataa
     1681 atccgttgtg tcaatttctg cggcaatgtt ggttttactt tgccaaccgg tcatggggag
     1741 cgaaatctca cccgcaacac cgtcagatga agacaactac acctttgacc cgcaactctt
     1801 ccgcggcagc agatttagtc agtcgtcatt agcaaaactg acaacacgtg agtctgttgc
     1861 accgggcaat tataaaatgg atatctacac caacaataag ttgtcaggca gttggaatgt
     1921 cacgtttaaa gaagccgctg atggtcgcgt tctgccctgc ctgacgcctg aagtcgcgga
     1981 cgcgataggc ctcaaaacag gggaagataa gggggaaaaa gatcctgtct gtacgtttgc
     2041 taaggaactc gctcccggca tcaccagcca gacacagttg tcacaattgc gcctggactt
     2101 atcggtgcca cagagtcaat tgattagtcg ccctcgcggc tatgttcccc ccagcgagct
     2161 ggataccgga gcatcgctgg cgttcatgaa ttatattgcc aactattaca acgttgccta
     2221 ttcagggcag aatgctcata gccagcgttc gctatgggca tcatttaatg gtggcatcaa
     2281 ccttggtgcc tggcaatatc gtcagttatc caacatgacc tgggataatg acaaagggaa
     2341 tcagtggaac aatattcgta gctatttgca acgcccgctg cccgccataa atagccagtt
     2401 aatgatgggg cagcttatca ccagcggaag atttttctct ggactcagtt atcacggcgt
     2461 tagtctcgcg accgatgaac gtatgctgcc ggactccatg cgcggctatg cgccgactat
     2521 tcgcggcgtg gccgcaacaa acgccagagt ctcggtaatg caaaacggtc atgaaatata
     2581 tcagaccacc gtggctcctg gccctttcga gataaacgac ctatacccca ccagctacag
     2641 cggcgatctg gatgtcaccg ttacggaagc taacggcgca gtcagtcgtt tcagtgtccc
     2701 cttttcagcc gtaccagaat cgatgcgtcc aggaacttcc cgttataacg tggaagtagg
     2761 taaaacgcag gatagtggtg atgactcgat gtttggtgac cttacctggc agcacgggat
     2821 gactaatacg ctgacattta acagtggttc gcgtatcgct gatggctacc aggcgctgat
     2881 gctgggcgga gtctatggca gttcgctggg ggcatttggg gcaaacctca cttggtccca
     2941 tgcgcgtgtt cccgaaagcg aagcgcagag tggttggatg tcgcaattaa cctggagtaa
     3001 aactttccag cctacttcaa ccaccgtctc cctggcaggt tatcgatact ctaccagcgg
     3061 ctatcgtgat ctggctgatg tgctgggaga gcgtcatgct gccagcaata aacagtcatg
     3121 ggactccagc cagtggcgtc aacagtcgcg cttcgatctt acgttaagtc agagccttgc
     3181 gaattacggc aacctgtttg tgtcaggttc aacacagaac taccgtggcg gcaagagccg
     3241 tgatacacag cttcagttag gttacagcaa tagctttagc catggcatca gtatgaacct
     3301 ttccgtcgga cgccaaagaa tgggcggcta taaagacaat tctgatgata tgcagacggt
     3361 aacatccctt tcattctcat tcccacttgg cggcaatgga cctcgtgtac caagtcttag
     3421 caacagctgg acccattcaa ctgacggtag ctcgcaatta caaagctcgc taaccggaat
     3481 gcttgatgaa gcacagacca ccaactacag cctgaacgtc atgcgcgatc aacaatataa
     3541 gcagacgacg cttagcggaa acatgcaaaa acgtttttca caaactaccg tcggattgaa
     3601 cgcatcgaag ggccaggatt actggcaggc ttcaggtaac gtacaaggcg cgatggctgt
     3661 gcatggtggc ggcattactt tcggacctta tctgggtgaa acgttcgccc tggtcgaagc
     3721 taaaggcgca gaaggtgcaa aagtctataa ctccagtcag ctggaaatta atgacagtgg
     3781 ctatgcgctt gttccggcag taacgcccta tcgctacaac cgtatatctc tcgatccaca
     3841 aggaatggat ggcgatgccg agttggtcga cagtgaaaga caggtagcac cggttgcggg
     3901 tgcggcggtg aaagtaattt tccgtacccg tcctggtaaa gcgttgctga ttaaatcccg
     3961 catggcagat ggttcggaac tgccaatggg agccgatgtg ctggatgaga ataatacagt
     4021 cgtcggtata gccggtcagg gggggcaaat ttacctccgc acagaacaga caaaaggcca
     4081 cttgtcagtt cgctggggtg aaggtgctaa cgatagctgc caattgccct ttgatatcag
     4141 cgggaaggac agcaatagcc ctatcatccg cctgaatgaa acctgtcagt cttgatacag
     4201 agaaaacccg atgtctaaat cgactttttt acacattctg attagctcca tcatattggt
     4261 ggcactaata caatcatccg cttgggctaa ctgtacaaac acacagatag gtcaaactga
     4321 agatggtcgg acagcgctta ttgagttcgg aaaaattaat atgaccgaca cctattttgc
     4381 gccagcaggt tcactcctcg cgacaacggt cgtacctccc actaactaca catcaggtgg
     4441 cgcgacagga agctccgtat tgtgggaatg tgatgcaaca gatttgccaa acatctattt
     4501 tctggtcgct accaatggcg acgaccgcgt ggggggcttt tacgatgcag gcgggcctga
     4561 tggtctgagt gatgtctatg ccacctggtt cgcttttgtc ggtctcaagc agaccatggc
     4621 gggcgtgacg cttggtcgtt actggaagaa agtgcccatc accagttatg ccactcaggg
     4681 aactaaaatc cagattcgct tacaggatat ccctcctctt catgctgagc tttatcgcat
     4741 cagtacgcta cctgatacat cagcaacaac aagttggtgc ggtaataata atacagatag
     4801 tagtggagtc ggattcgcaa aaccttccgg tacaatctat aactgtgttc agcccaatgc
     4861 ctatattcag ctttccggta ccagcggcat tttatttggt catgatgagc ccggcgaaga
     4921 tagttctgtt cattgggatt tctggggtgc tgataatggt tttggttacg gaatgcgttc
     4981 ggccaatcga ctctacaaca atgccacctg cgttgcccgc agcgccacgc cgttagtatt
     5041 gctgccgaca attgcagaag cacaactgaa tgcgggcatg gaaagtaccg gtaattttaa
     5101 tgtccgcgtc gagtgtagta actcggttca atcagggatt agcgatactc agacagcatt
     5161 aggaatccag gtgtctgaag gtgcatatac agcggcgcaa aaactgggga ttatcaatag
     5221 caacggcggc gtcagcgccc tggtctctga taattatgac gcagcagaga tggcaaaggg
     5281 cgttgggatc tacatttcta acagtgctca ccccgatacg gcgatgacgc tggttggtca
     5341 accgggcatc gcgaagttaa cccccggagg aaatgcagcg gggtggtatc ctgtatttga
     5401 aggggcaaca ttagaaggtg cgactcaccc cggatactcc agctatagtt actcttttat
     5461 cgcccggttg aagaaactgc caaatcagac agtcagtgcg ggaaaagtgc gggcaacggc
     5521 ttatatattg gtgaaaatgc aatgaaatcc gttttgctgc tcttaaccat cttgttcgta
     5581 cagtcagtgg aagccggcat catgccggct tcttcacgga ttatttatcg cgatggcgat
     5641 cgggaaaaga cactgatgct ggtcaatacc aatgactatc cggtactggt gcaaagttgg
     5701 gtagataatg gtgaaggttc cccagaagct gcttctcccc cctttgtggt gctgccaccg
     5761 gttttccgcc tggctgcgag acgaacccag gggttgcgga ttatctataa tcatgaccca
     5821 cttccccagg atcgcgaatc tgcattctgg ttaaatcttt atgaaatgcc gccagaaact
     5881 ccccatcaag atcccacaca tttaacgctg gcaatgaata cgcaacttaa aatcttttac
     5941 aggccaaaat ctgtaactat gacgccccaa gaagctatgc gtaaaataag ttggcatctg
     6001 ggtaacgacg gcagcagtag ctatattgag cttaataacc caacaccact gaatatctct
     6061 ttcacctcca tcagcattgg ggctacaaag gtgcagcaag aaagcgatat gatgataaaa
     6121 cccttcagtc aacgacggta ctggttagca agtaataacg ttagtggtgt ggttgcggcg
     6181 acatatctcg atgataatgg tgcacagata aaacaacaat tagcgcaata atgctctgag
     6241 tggtatgtaa actcatcgcg atggttcaac gctaacgaat aaccactata cccacctgaa
     6301 gtaccggaag tggagaaatg acaaaatctc tccactccat tttccagtca aaacacctca
     6361 acgcagccaa aaatcgttaa cgaattgaat atccaccatc aatgactaat agttgtccat
     6421 tgacataatc tgaagcggca ctggcgagga acaccattgc gcccataaga tcctgtgccc
     6481 ttccccagcg ttgagccggt atacgccctt ttattaactc ttttatttta gggttattat
     6541 aaataatagc ggtcatttca ctcacataat atcctggggc gataccatta atctgaatat
     6601 tatattccgc aaaatcatcg gcatacgctt tggtcagccc cgctaaagca tgtttagtcg
     6661 ccgcataccc tggcgaccaa cgtcccccat gaaatgacaa tacggaacac atattaataa
     6721 ttttcccttt tctttgcggg acaaatatat tcaatgcggc ctggctcata tcgaatgcag
     6781 cattcaggtt gagattaatc atcggatccc agtcgtttcg attgaaatcg ataggacgat
     6841 taatgttaca cacgcccgcg ttattgacca gaatatctaa ctgaccgaaa cgctccagac
     6901 acctattaat aatggtgcat gatgcatcag gttttgttat gtctattgtc ataaactcaa
     6961 cttcaacgcc ttcatgagtt aaatgcttca aatatctatt atcaggctct ttgatgtcaa
     7021 ccaacaatag attagcccct gctttggcca gagccgagca taagcctctc cccagctcac
     7081 ccgcggcccc agttacgatg gccaccttgt tatgtaatga aaaaaaatcc aatgaaaaat
     7141 cttcaataga aaagctcata tcacacattg ctccttatga gtaagtaata tttaagggat
     7201 aatttaagta atttttagat ttcaataaaa aaatataaac attaaaactc agcatgaata
     7261 ataaacatca aatcttatat acatttaagt agtgatgtat cacgtaaaaa tatagtcatc
     7321 atagatgtta tgcagtacgc acaaaaaaac agactggatt tgcttgctga tctataatct
     7381 ttatctaaat tactattggc gctttggata ataacataag catatattac ctcctagcaa
     7441 taatagatat aacctcatat caaggtgtca ctggtaaata atcggataaa agacagtacc
     7501 ggtcggcaca tagagcttct tctatttcac cagaagtgca cagcagggaa ctaccaatca
     7561 cacggttagt gtcatgggtg gaggatgcgt cccgttcttt ccctataacg gggatacgca
     7621 gtttttatca gtacatgcag aaaaacccct aattcacatc actgcagaaa gcatttaata
     7681 gaaatataca tcgtttaaga atttcaatta aatcaagatt attcttaaaa atcagataat
     7741 tcag
//
LOCUS       DAZFDC010000175         7662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFDC010000175 DAZFDC010000000
VERSION     DAZFDC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.175"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(93..356)
                     /gene="cedA"
                     /locus_tag="ACHJ48_004318"
     CDS             complement(93..356)
                     /gene="cedA"
                     /locus_tag="ACHJ48_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO4709746.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            539..2800
                     /gene="katE"
                     /locus_tag="ACHJ48_004319"
     CDS             539..2800
                     /gene="katE"
                     /locus_tag="ACHJ48_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO4709747.1"
                     /translation="MSQQNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTASGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2847..3605)
                     /gene="chbG"
                     /locus_tag="ACHJ48_004320"
     CDS             complement(2847..3605)
                     /gene="chbG"
                     /locus_tag="ACHJ48_004320"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO4709748.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMNFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3618..4970)
                     /gene="celF"
                     /locus_tag="ACHJ48_004321"
                     /gene_synonym="chbF"
     CDS             complement(3618..4970)
                     /gene="celF"
                     /locus_tag="ACHJ48_004321"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO4709749.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5076..5918)
                     /gene="chbR"
                     /locus_tag="ACHJ48_004322"
     CDS             complement(5076..5918)
                     /gene="chbR"
                     /locus_tag="ACHJ48_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO4709750.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5926..6276)
                     /gene="chbA"
                     /locus_tag="ACHJ48_004323"
     CDS             complement(5926..6276)
                     /gene="chbA"
                     /locus_tag="ACHJ48_004323"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO4709751.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6327..>7662)
                     /gene="chbC"
                     /locus_tag="ACHJ48_004324"
     CDS             complement(6327..>7662)
                     /gene="chbC"
                     /locus_tag="ACHJ48_004324"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO4709752.1"
                     /translation="EKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVF
                     LSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEER
                     KVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVR
                     RNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPL
                     ASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAAL
                     AAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQIN
                     EPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFF
                     NTNGSVVALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
BASE COUNT         2048 a         2008 c         1716 g         1890 t
ORIGIN      
        1 ctttttgtta caggtggttt gaatgaatgt agcaatgaaa tagagaattt caggccatgt
       61 aactcccggc aaaaccggga ggtacgtaat ctttactcag tcacttcccc ttcctggcgg
      121 atctgatttg cccaacgctg ggcggattca ggcacagtaa acgccggtga gcgcagaaat
      181 gactctccca tcagtacaaa cgcaacatat ttgccacgca gcatccagac atcacgaaac
      241 gaatccatct ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac atagctaata
      301 atctggcggt tttgctggcg gagcggtttc ttcattactg gcttcactaa acgcatatta
      361 aaaatcagaa aaactgtagt ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat
      421 ttcataacac cgtttccaga atagtctccg aagcggggtc tggctggtgg tctatagtta
      481 gagagttttt tgaccaaaac agcggccctt tcagtaataa attaaggaga cgagttcaat
      541 gtcgcaacaa aacgaaaaga acccacatca gcaccagtca ccactacacg attccagcga
      601 agcgaaaccg gggatggact cactggcacc tgaggacggc tctcatcgtc cagcggctga
      661 accaacaccg ccaggtgcac aacctaccgc ctcagggagc ctgaaagccc ctgatacgcg
      721 taacgaaaaa cttaattctc tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac
      781 cactaatcag ggcgtgcgca tcgccgacga tcaaaactca ctgcgtgccg gtagccgtgg
      841 tccaacgctg ctggaagatt ttattctgcg cgagaaaatc acccactttg accatgagcg
      901 catcccggaa cgtattgttc atgcacgtgg atcagccgct cacggttatt tccagccata
      961 taaaagctta agcgatatca ccaaagcgga tttcctctca gatccgaaca aaatcacccc
     1021 agtatttgtt aggttctcta ccgttcaggg tggtgctggc tctgccgata ccgtgcgtga
     1081 tatccgtggc tttgccacca agttctatac tgaagagggt atttttgacc tcgttggcaa
     1141 taacacgcca atcttcttta tccaggatgc gcataaattc cccgattttg ttcatgcggt
     1201 aaaaccagaa ccacactggg caattccaca agggcaaagc gcccacgata ctttctggga
     1261 ttatgtttct ctgcaacctg aaactctgca caacgtgatg tgggcgatgt cggatcgcgg
     1321 catcccccgc agttaccgca ccatggaagg ctttggtatt cataccttcc gcctgattaa
     1381 tgccgaaggg aaggcaacgt ttgtacgttt ccactggaaa ccactggcag gtaaagcctc
     1441 actcgtttgg gatgaagcac aaaaactaac cggacgtgac ccggacttcc accgccgcga
     1501 gttgtgggaa gccattgaag caggcgattt tccggaatac gaactgggct tccagttgat
     1561 tcctgaagaa gatgaattca agttcgactt cgatcttctc gatccaacta aacttatccc
     1621 ggaagaactg gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca atccggataa
     1681 cttctttgcc gaaaacgaac aagcagcatt ccatccaggt catattgttc ctggtctgga
     1741 cttcaccaac gatcccctgt tgcagggacg tttgttctcc tataccgata cacaaatcag
     1801 tcgtcttggt gggccgaatt tccatgagat tccgattaac cgcccaacct gcccttacca
     1861 taatttccag cgtgacggca tgcatcgtat ggggatcgac actaacccgg cgaattacga
     1921 accgaactcg atcaacgata actggccgcg cgaaacaccg ccgggaccga aacgcggcgg
     1981 ttttgaatca tatcaggaac gcgtggaagg aaataaagtt cgcgagcgca gtccatcttt
     2041 tggcgaatat tattcccatc cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg
     2101 ccatattgtc gatggtttca gttttgagtt aagcaaagtc gttcgtccgt atattcgtga
     2161 gcgcgttgtt gaccagctgg cgcatattga tctcactctg gcccaggcgg tggcgaaaaa
     2221 tctcggtatc gaactgactg acgaccagct gaatatcacc ccacctccgg acgtcaacgg
     2281 tctgaaaaag gatccatcct taagtttgta cgccattcct gacggtgatg tgaaaggtcg
     2341 cgtggtagcg attttactta atgatgaagt gagatcggca gaccttctgg ccattctcaa
     2401 ggcgctgaag gccaaaggcg ttcatgccaa actgctctac tcccgaatgg gtgaagtgac
     2461 tgcggatgac ggtacggtgt tgcctatagc cgctaccttt gccggtgcgc cttcgctgac
     2521 ggtcgatgcg gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca acggcgatgc
     2581 caactactac ctgatggaag cctacaaaca ccttaaaccg attgcgctgg cgggagacgc
     2641 gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt gaagaaggga ttgtggaagc
     2701 tgacagcgcc gacggtagtt ttatggatga actgttaacg ctgatggcag cacaccgcgt
     2761 gtggtcacgc attcctaaga ttgacaaaat tccggcgtaa atatggataa ggtcacgtcc
     2821 cctggggagt gaccttacca ccttacttac acatcaagat aactacccaa ccgataacca
     2881 cgctcagcaa ttgcatattt caacgacgct gatgtcagca cttccagctc cgttaaccgt
     2941 gggaaacagt aagcactctg gcgaatcgta ttatcgacaa acgccggatg gcacatgact
     3001 tccagcgagc gttccccgcg atggctggaa tcatcaagca cttgcaggaa cagtgcctcg
     3061 ctaatctctt caccataaaa tgcgctactg aatccgtgcg aactgcgcag attagccggt
     3121 aaatcaccag cgttagatag cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc
     3181 gcaaacctgg caacaatagg gaaaatctgc gggaacatat gcacatgatg atggctatca
     3241 agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact ggcaagctcc
     3301 tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat ccattttccc
     3361 agcacaccat cgcgggttaa ccccggcata gctgtcagtg gcttgcccat agtaaggaca
     3421 aagttcatcc ctatggccag actcggttca tcacgactca actgcaccgc atggtcaata
     3481 gcctgcccat tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca ggcctcgata
     3541 atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat cagtaagcgt
     3601 tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt
     3661 ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg
     3721 gaatgcacca acggactaag gtttagcgcc agtaacacat cgttaaattc tccgctaagt
     3781 gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc catcacttta
     3841 tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag cgtacaggtc
     3901 atttctaccg cccagtctgc cggaatatta tcaatatgcc catgatgcgg gatattaacg
     3961 taatgttctg cttgcttatc gttgtagata gcgttgatca cttcgcacgc tgcatcagag
     4021 taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc cgggttttta
     4081 tacagctcaa aaagttgttt ctcgactttc tgtactacct gcgctcgtgc gccgcctttg
     4141 tagtattcgc ccatttcaat agccagcatc tctttttgct tgaagtaata aagcaaataa
     4201 gaacacggca acagattcag agaacgaatt aagccttcgc taaatggcag atcgaaaata
     4261 tttttaacgc cagatgcttt taactgcccg gatgccacac catcaagcaa ttcggcaaag
     4321 cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag gccgaacaga
     4381 tcgatagata aatcatcgct gtctttcagc atcagaacat cgcgaataaa catcttcatg
     4441 ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg ataaacggct
     4501 tcagtgacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg acaaagttct
     4561 tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt aaacagaccg
     4621 cccgcgccgt tggtttcctg accaagataa ccatgactta atggaatacg ttcatccagt
     4681 tcacgcgccg gtaattggcc aacgcgcagt tgggtagtaa cgaaatcagc atctttcaat
     4741 gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt atcaatcatc
     4801 cgttggcaga gatcgaaaat aatatccagt ttagctttgc caccttcgac atccaccagc
     4861 cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag taactccggg
     4921 gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat aatttctccc
     4981 ttcagtacag aatactgata tctggcatat ctgccccccc cggacataaa taatccagca
     5041 acaggacaga tatgtgaatt gtcaggtata acgacttact gattaaattc agtcaatttc
     5101 ttacgatagc tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc
     5161 gggctactat aaccggcctc aaacgcaata tccgtcactg aatagttggt catttccagt
     5221 tgttttttgg caaaattaat acggatttca ttaataatct gcattggcgt tttgccataa
     5281 tatcgttgag tcgcccgcgt caaatattcc tgtgatttgg ctgacaacgt caccatattc
     5341 tccagcgccg attcactaaa ctgctcttta tcatgcatct tttctaccgt acttttcagc
     5401 cactgcggta catcatcaat cacctgttct tcgcggtaat gacgtaaacg gttaatgaca
     5461 taaaaagtaa ccatctcaac aaactcttcc agccctgttt cgcggaaatt caatgaagaa
     5521 atcactgttt ccacataggt gagaaaagcg ttattggtcc ggtatacctg cgaagctaca
     5581 aagcaataag gcaacaatgg caggtaatgc tgctcaaaaa agcgtttact gatcccaacg
     5641 ttcaatatgc gcgtggcacc aaactcataa aaactttgat ggtgcgaacc taacggaata
     5701 aaaacaaaat cgccccgttc cagtaacacg cgcttaccgt taatctcctg gaaataacgc
     5761 ccggttaata ccagagtaaa ttcataatag tcgtgctgat gcagtccgct gatactctca
     5821 gttttgttat agataaacac atggaagttt ttgccattaa acaactgctg ttctcgggca
     5881 gtggcaattt ccggcgcgtt aatcactggc tgcatcatcg actccttatg ccttcagttt
     5941 ttcatgaagc tcaattaatt cagtaatcag ttcacgcgca agcatggacg tcattaaatg
     6001 atcctgagcg tggaccagca ccagactcac tttcatctta ccttcgcccg catcgccttc
     6061 aatcagtttc gtctgtacca gatgcgcttc attcaatgcc atccgtgact gatccatcat
     6121 ggcttttgct gcggcaaaat cgccctgctt cgcctgtttc agtgcggcat acgccaggct
     6181 gcgcgcttgt ccggagttga tgataagccc catcaccact tcttccagct cttcagcttc
     6241 cgtttgcgta tcgggaatat tatcgagatc catcatacat cgttcctctc ttttcttacc
     6301 ggcacgatta cccgtaccgg catcgattaa aatttcaggg cgttagcgat atcttcttcg
     6361 ctctcttctt tatcaatcgc attttgtgct ttgttagcca ccacaacaaa gggcagataa
     6421 attaacgttg cgataccaag gttgaagagt gcaaccagca atgcgacgac gctaccgttg
     6481 gtgttaaaga aggctcccag accggttggc atggtccacg gtgcaatatt ggtcaccgga
     6541 ggaataatgc ccatgtagta cgctgccagg gtgattgccg ccagaatcgg ttgtaccagt
     6601 acaaacggga taaacatcac cgggttcatg ataattggca gaccaaacag aatcggttcg
     6661 ttaatctgga agatgccgga cggcagcgcc agttttgcca cctgacgata atcagcacgg
     6721 cgagaggcaa taaagatagc caggatcagg cctaaagtcg caccactgcc tccaaggaaa
     6781 ataaaggagt ccagcatcgg cttggcccag atatggaagg tcttaccggc tgccagcgcc
     6841 gcttcgacgg aaccatattg ctgataggtc gcgatatttt ccagcgccca cggcgtcata
     6901 atgccgttgt ccagtgcggt cagcgccagc gcgccatgaa taccgaagaa ccagagcagt
     6961 ggaacaaaga tcacataggc ccagcccacc acgctaccca acgatgccag tggggttgag
     7021 atggtatcca taatgatctg atggaagttg gtgccccagg tattcaacgc ccaggcaata
     7081 atccccatca cggaaagaat aataaagccg ggaattaatg ccgagaagga acgcgatact
     7141 gaagcaggta cgctgtcggg cagtttaatg acccaattgc ggcggacaat aaaggtaaac
     7201 atttctgcca ccaccaggcc aataataatc ccggagatga tattcgcccc acctaaccag
     7261 tttgcgccaa ccgcataggc ctcaccgaca ctatatgggg tgacggtcat aaatgctgca
     7321 acggataaca acccagccgc cagcgcatcg actttacgct cttctgccag cgccatgcca
     7381 ataaagaacg gtgccattaa agacattatc cctaatgttc cgttatatac gttgccgcca
     7441 atacctttca gaccattaag tgtttcaatg gttgaggcgt cgaggcgaat acctaaggaa
     7501 taaaaaaacg acccctcccc aaagcttaga aaaacgttgt taattaatac aaacatggcc
     7561 cccgcaaggg ttaacggcat taagcgaata aagccatttt tgattgcatt aacgtgtggc
     7621 tgctttccta ttttaactgc aaaagggagg agtacctttt ca
//
LOCUS       DAZFDC010000176         7589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFDC010000176 DAZFDC010000000
VERSION     DAZFDC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.176"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..4755)
                     /locus_tag="ACHJ48_004325"
     CDS             complement(<1..4755)
                     /locus_tag="ACHJ48_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4709753.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQRAMAASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSKNVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGNSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDVISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDTQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARLDYHLFYEYDAADRIIRWSDNDQT
                     WSRFTYDAQGRCVTVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPDGNILREEAP
                     DGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGADWQYCYDERGLL
                     SNITAPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQDERRLGIEYD
                     HHNWPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAGTGAE
                     GFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVIN
                     PQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETILYSRDAAGRL
                     AEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDE
                     HTVIRCILPPEDERDRHPDGSLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVL
                     RHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLLREYRYDSAGNVSGVTSR
                     EDYGRETHREYRLDRNGQVTAVTASGTGLGYGEGDETYGYDSCGYLKAQSAGRHRISG
                     ETDQYAAGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSRDQLTGYRSA
                     QGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGF
                     ELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGL
                     ALSLPADTDYPDPRGERDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDP
                     LGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNSLGNRALKYA
                     NGDWIKSEAAFNKYINMINKRLELTGSKFRVEIQPAIKNGERVPATTNGPFKVNGKWT
                     SGTHYTGGSKRLDAGIIDITSPTNQYGLHPVIE"
     gene            complement(4775..5236)
                     /locus_tag="ACHJ48_004326"
     CDS             complement(4775..5236)
                     /locus_tag="ACHJ48_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO4709754.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREPVSQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5264..7165)
                     /locus_tag="ACHJ48_004327"
     CDS             complement(5264..7165)
                     /locus_tag="ACHJ48_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4709755.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALENHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1465 a         2267 c         2010 g         1847 t
ORIGIN      
        1 ttcaataact ggatgtaatc catattggtt tgtaggagat gtgatatcaa taataccggc
       61 atctagacgt ttggaaccac ctgtataatg agtgccggat gtccacttac catttacttt
      121 aaatggtcca ttcgttgtcg caggaactcg ctctccattt tttatggctg gttgaatctc
      181 aactctaaat ttactacctg ttaattctag tcttttattt atcatgttta tgtatttatt
      241 gaatgcagcc tctgatttta tccaatcacc attagcatat ttaagtgctc tattccctaa
      301 tgagttcaaa acatcagata tgttgctttg ctcactcccc catttcgtcc agcgggctaa
      361 ctgacatata gccagcccca aaggatcgat atacccacaa ggattcggca cataccgata
      421 agtctgctcc cctccctgca accccagcgg atcactcacc aggtacatcc cggtttccgg
      481 ctcgtagtac cggaaccggt tatagcacag ccccgattct gcatcctgcc actgtcccgc
      541 atacagcagg ccggggtccg cttccgggtc ccgctccccg cgcgggtccg ggtagtctgt
      601 gtctgcgggc aggctgagtg ccagccccca caggctggtc tgccccggcc gccagacggt
      661 tttaccttca ctgttaaaga gcatcagcgg gcggcccgtc aggtcgctca ccgcatgatt
      721 cgttcgcgtc acccactgcg gggccacgga cggatgcggc tgtcgtaccc ggctgaactg
      781 ctggcttatc agctcaaagc cgttaaacac caggtgcctt acgctgtaca gtttatcatc
      841 gcggtattcc cggatttccg caatactgtc gccgtcccac aggtacgtaa aacggatttt
      901 cttccggtcg cagcgttttt ccgtccgtct gccgctggcg tcgtggcggt attcccactg
      961 ctccccctgt gcgctgcgat acccggtcag ctggtcccgg ctgtcccacc ggaaccgctc
     1021 tgtttctggg cggtagccgt cacggtgttt tgtgcggctg accatccggc ctgcggcgtc
     1081 atagtcatac tgtgtgtttc cggcctgttt cagccggtgg cctgcggcat actggtcagt
     1141 ctctccgctt atccggtgtc tgcccgcaga ctgcgccttc aggtagccgc agctgtcata
     1201 gccataagtc tcgtcgcctt cgccatagcc cagcccggtg cctgaggctg tcaccgccgt
     1261 gacctggccg ttccggtcaa gccggtactc ccggtgtgtt tcccgtccgt aatcttcccg
     1321 gctggtgacg cctgatacgt tacccgcgct gtcgtaacgg tattcccgta gcagtgaagg
     1381 gagcagtccg ccccatgtgg cttccgccgg gaaaagtccg gcgcgctggc tgacaggctg
     1441 accggctgca ttccagccct gttcacaggc aaaaccccgg ttactgtgcc ggagcacttc
     1501 acgtcccgcc tcatcacggc tgaacgtcag cgtctcatcc cccggcagga taacctccgt
     1561 cagttctccg gcggcgttgt agcggtatgt ggttttcagc agggatccgt cggggtgtct
     1621 gtcgcgttca tcttccggtg gcaggataca gcggatgacg gtgtgttcgt cggggtagtg
     1681 gtattcaatg gcccgtcggc cctgagtggt gacattcacc cgcccgcgtt catcatagcc
     1741 ggtgcgctgt gatgtgccgt ccgggctgaa gatacccgtc agcctgccgg atttgtcata
     1801 cgcatactgc gttttgcctt ccggagtgat gacttctgcc aggcggcctg cggcatcccg
     1861 gctgtagagg atggtttcct ccaggccgtt gaccctggcg gtacacaggc catcagcgtc
     1921 ataacggtaa cgacagacca cgccgcccca gtcacgttct tccgtcaccc ggccacagtt
     1981 gtccagaata taaagataag actcgccctg cgggttaatg acttctgtca gttgcagcgt
     2041 gtctttatcg tagcggtact gccagcggtg gccttcaccg tccgttcgcg ccaccggcaa
     2101 atcaaacggg ccgtactcca ttgtccagac aacgccgtta ccgtccgtgt acgccagcag
     2161 gttgccgtgg cggtcgtagc ggaatccttc cgcgccggtt cctgcccgct caaggccact
     2221 cagtttatgg tgctgctgcc actgcaaccg cactgactga ccgccggggc cgcgcatctt
     2281 caccggcagg tcgtgaccgc tgtattcggt gtggtgttcg cggcccatca cgtcggtgag
     2341 tgtttccggc cagttgtggt ggtcgtactc tatgcccaga cgccgttcat cctggcggaa
     2401 tatccccgac agcagcccct gaggggtgta cgccagccgg gtctcttcgc cctgtggcga
     2461 taccagactc accggcaggc cgcgttcatc acactgctgc gtccacgtct gcccggcagg
     2521 ggcggtgatg ttgctgagca ggccgcgctc atcatagcag tactgccaat ccgcgccgtc
     2581 tgccgccgtg taacggctta actgaccgtg tgcggcgttg tattcaaact tctccacccg
     2641 cccggcaggg gagtggcggg ccagcaggtg atggaattca tcccattcat acgtggtggt
     2701 gctgccgtcc ggcgcttctt cccgcagaat attgccgtca ggatcatagt aataacggtg
     2761 aatgcccttg ccgtccgtca cggtggtgca gccgtcacca tagtccagcg tggcgttgta
     2821 atagccctcc gccccggtga cggtcacgca ccggccctgt gcatcgtagg tgaaacggct
     2881 ccacgtctgg tcgttatcgg accagcggat gatccggtcc gcagcgtcgt actcataaaa
     2941 caggtggtag tccagccgcg catcctgttc gtaggtcgcc agcgtctgta tgccgttatc
     3001 cgtgcggtga atggctttca ggtagccgct ggcgtggtac gtcagtttaa gccggatccc
     3061 gtcggtgtga ctgacacccg tgatccggta ctgggtgtcg cgctcaaacg tgatgcggtt
     3121 gctggcggtg tcgctcatcc cgcccagcat gtacatccgg tctgcggggc tgtgtgccag
     3181 gcggtcatcg ctggtatcgt cagtcaccgg gtggcgcaga cgcattcccg gaaaggcgtc
     3241 ataaaagaag cggctgctca gtgcatccct gtcgaacagc tccagataat catcacggcg
     3301 gcgcagaatg tactgggcgt gatacgggtt aaccgccgta tcactttcac cgtcgaaggc
     3361 gaagttcagc gtggctccca ggctgatgac atccacccgg tttccctgtt cacgcaccca
     3421 ggcgtattcg ctccaggagt cgctccagcc cacgcccgtc aggccatgca gcggcagtac
     3481 agaactgtgg aaacgggtga aggagagggg gagtgtctgg cccagcgtaa agtcggtacg
     3541 ttcgtcgcag tatgcgccgg ttaccggatc gacaggatct gttacccatc tgatgacttt
     3601 attggcaata ctttttaacg aattacctgt tttccccagc aacgcgccga gctttgcgcc
     3661 cagcagctta ccgcttttcc ccagcaggtt tcccagtttg cccagtttcg ccagattccc
     3721 cagttttccc agcagtcctg ctggcggcag aagatagctg gctatcgtta ttcctcccag
     3781 gatcattctc tgccatggcg ggaactccgg ctggatatcc agataggtga cctgctcacc
     3841 cccaataaag acgtccggcg aaccttccac gattgccgcg ctgcactcca gtttgtcgcc
     3901 cttgcgcgcg gcaggctgat tgttgataaa gacgttgctg ctgccctggg ctatcctcgg
     3961 tggtttgttc tccttgtcgc agagcgccgt gtcctcaccg gcttccggga aatccacatc
     4021 ttttttgatt tcgccacgcc cgcccaccat ggtggcaaac tccattaata ccggaccggc
     4081 gggaaacaga agcgatgcgc cggcgctgac gcgatccata aaaccggcat tgctgtccgt
     4141 cagggtgata acagcgttcc cccaggcctt cgccagtggt actgcttttt ccgctgccga
     4201 ttcaagcagg tcagtggcaa tatcacccca tgagggttcc ccctgtggga cttcaggtat
     4261 tgctccaggt gggggaggag ggggaagggt gacggctgca cgggctgctg gctttccgtt
     4321 aatttttaca tttttagaac cggtattgat ggcgccgtcc gtgtcacaaa agctgtttat
     4381 ccatgattca agctggttct gtttctcctc cacataatca ctcgccttat agctggcata
     4441 catcagcgca ggcaccagaa ccagcgcata agggccggtg gcacaggcgg ccataatcag
     4501 gcaaccttct gctatggtca ctatcgcccc cagcacggcc cctgccagcg cccccaaaaa
     4561 actcttgtgc tgaatttcat cgcccagcct ggcggcgggg gggccagact tttttgatgc
     4621 caacatctgt gcaatggctt tgtcgttggc tgcccgtttc atctggctgg cggccatcgc
     4681 ccgctgcgac agcatccgca ttgccagcgt accgcctgcg gtcgctccct gtggcccgcc
     4741 tggtccttca ctcatgtttt atccctcgtt tatgtcagcg cggctggtcc ttttggatgt
     4801 cattctccgg tggcaacggg gtgaagctct ttatcacctc cagcagcatc gtcttttgac
     4861 tgttcttcag ggataaggag gtgctgaaag tgaaggtcag cagcgtatct ccacgccgct
     4921 ggaatacggc tatctggcta atccgcccct gattcccttc atcttccgga ccgttccagg
     4981 aataatccag cagccaggcc ggggcctctg ccagagtgat ttcccggcag gcatgttcgg
     5041 tgtacccatc aagtttgccc tggtacagcg ccagcgtttc ctggtacagc tctggtgtca
     5101 gggttccctg tgaaacgggt tcgcgggtaa tgaccaggtt gatgccactg ttatccgtgg
     5161 ataccaggac ctgcatcgac tgatcctgcc attgcgatgg cagttgaata tgaccttcat
     5221 taaaagtgaa ctgcataaaa tactccctgt taatctgagt gaatcagttg atgaaaacag
     5281 aaccgcccac gatggtgatg ccatccttcg acagcctgat taccgttgat tccccgacct
     5341 gtagctgaat gccatccggc gtcagcgcaa ttttgctttc ctccacggcc agcataattt
     5401 ccttaattgc cgtataagcc tgtataccgt tgccttcgcc accattgttt acagcgtcaa
     5461 catgcaccga accggcaatc gtgcctctgt cctgaaagac atcctcagat aaagaccctt
     5521 tcagtactcc ggtgatcttg tggccttttt ccaccgtaag aaggcggtta ccttcctgga
     5581 tcgtgaccgt ctggtcattt tttacggttt cgcgatgatc gtgttttacg gtggtggtac
     5641 ggtcgttgag cacttcggta tccatgttct tctgcgcgtg gatgtagacc tgttctctcc
     5701 ctgtcgcatc atcaaacttc agctcattaa acccgctgcc catatacgtt ttggaacgga
     5761 tggtcatctg cgtcttcgtc cccggcaggc tgccgggggt gcggttttcc tggtggtagg
     5821 tgcgccccat aatgatcggc tggtccggat cgccgttgag gaagtccact atcacctcct
     5881 gacccacacg cggtatcgcc aggttgccaa aaccggtgcc tgcccacgcc tgtgccacac
     5941 ggatccagca cgagctgtcc tggtctgccg ggttataacg gtcccagttg aatttcaccc
     6001 gcactcggcc atgttcatca cagaagattt cctcaccctc cggccccgtc acgacggcgc
     6061 tctgcggacc gtccaccagc ggttttagcc gcggctgggg tcgccatgtc cggtctgccg
     6121 ggatcaccgc aaaatggttc tccagcgccg tccctgctcc ccgccttccc ggcaccgcct
     6181 gcggctgctc gccgtgcagt tcacttgcca ccacctgcca ttcccggttc aggttcgcct
     6241 gcggatgccc cgtcagcaca attcgccgtc ccggccatat ctccggcgag cgactcattc
     6301 cccgcgcggt ttctgcattg tttcgccagc cctccatctg ccagcgggca aagttctgcc
     6361 cgtgggcgcc cttgaaacgt ccggggtagt catacacctc atactgcgtg cgctggtaat
     6421 cctggtgctg gccttcctga tcaaaacgtc ctgcccagcc ggggcgttta aaggtgtagt
     6481 ctttggtcac cacggaagaa gggcggattt gtgcgctgta gcgaaactgg ctgatgcaga
     6541 gggtgctcac ctcggtacgg gtgttcgggt tccaggggat ttcaaaagat tcgggcagat
     6601 ggcggaccgt gtcgcacagc accaggctct ggtcggtact tttgtaagca tgctcctcat
     6661 aaaagaagat gccttcctcc gccgccatcc ggcacaggaa atcgtaatca gtctcaccgt
     6721 actggacaca aaactcacgg gaaggatgcg gttcactgaa cagcggactc cactcggtca
     6781 ccccgttttc ctgcaatatc gtgccgagga tgcttttgat gtcctcgttc tggaagatac
     6841 ggaagttctg gcgcagaccg gcacgccaca gcggcgggtg caccttcatg ctgtacagca
     6901 tctggttttt gtcgttctcc cccagttcaa accaggtcac cacacctttc acccggcgct
     6961 gtacatcatc accctgccat attgtcaggt aggccatttt gtccagcacc tgcgcaaatt
     7021 caagggagag aaactgctgg ctgaccaagg agagatcgag ggaaaagagc gaagagagtg
     7081 actgggtgag atgaaaggaa accaccgcaa aagcgtccgg tggcaggccg tccacttcca
     7141 gtgtgaaacg taatccggtt gacatagcaa cctccggtaa aatgaataat aatccgcacc
     7201 gtacaactgc ggataatgag ataagcggga cgacttaaga cgttattagg gaaggaacaa
     7261 aaaatgctat gattatgtga ggattttgtt taaatattac tttacagaaa cgggtgtctg
     7321 ttcagtcaaa aaaataatca caagctcaac ttattgtttc ctgacgttat tcaggacagg
     7381 tgcgcacatc acagcgagta acaaaaacgc aaagttaaac caatattcat aatatgtttt
     7441 tattcacaac taaataaccc aattaacatc attaaaatga atgattgctt aaaaaatata
     7501 tgctaccgct ccaccctgat aacatgatct gcacattgca catcttaacg ttgttaaata
     7561 ctggctcaat agtggatatt ttaaattag
//
LOCUS       DAZFDC010000177         7529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFDC010000177 DAZFDC010000000
VERSION     DAZFDC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.177"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(278..763)
                     /gene="sixA"
                     /locus_tag="ACHJ48_004328"
     CDS             complement(278..763)
                     /gene="sixA"
                     /locus_tag="ACHJ48_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO4709756.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(966..3110)
                     /gene="fadJ"
                     /locus_tag="ACHJ48_004329"
     CDS             complement(966..3110)
                     /gene="fadJ"
                     /locus_tag="ACHJ48_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO4709757.1"
                     /translation="MEMTSAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVAHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3110..4420)
                     /gene="fadI"
                     /locus_tag="ACHJ48_004330"
     CDS             complement(3110..4420)
                     /gene="fadI"
                     /locus_tag="ACHJ48_004330"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO4709758.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVVESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4600..4884)
                     /locus_tag="ACHJ48_004331"
     CDS             complement(4600..4884)
                     /locus_tag="ACHJ48_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO4709759.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5256..6596
                     /gene="fadL"
                     /locus_tag="ACHJ48_004332"
     CDS             5256..6596
                     /gene="fadL"
                     /locus_tag="ACHJ48_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO4709760.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRVFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6961..>7529
                     /locus_tag="ACHJ48_004333"
     CDS             6961..>7529
                     /locus_tag="ACHJ48_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709761.1"
                     /translation="MLSSISINNTSAAYPESINENNNDEVNGLVQEFKSLFNGKEGIS
                     TCIKHLLELIKNAIRGNDDPDRININNSSVTYINIGSNDTDHITIGIDNQEPIKLPAS
                     YKDKELVRTIINDNIVEKTHDDINNAEFTDGGIDDRSDIGIDSNDTDDIDIDNQEPIK
                     SPANDENKKVGQTIINENIVENTHDINNK"
BASE COUNT         1996 a         1951 c         1712 g         1870 t
ORIGIN      
        1 aagttatact attaacacat cacaaataat attaaattaa gacacgaaaa tatcatttct
       61 tttttattat taactgttct attagtttga tgctttgtaa aaatacatta caacttagtc
      121 tcatttcgtc agaaattaat agggaagcag aggaaaaagt gcgatgaatt ctgtatcgaa
      181 gcgtatcaac tccgtgaagg gttttgagtg aatttctact attgcaaaaa gacaaatgcc
      241 cgatgtacag cttacatcgg gcattttttc aaacgaatca gatagctttc gccatcttca
      301 aattacatgg gctcatctgc cagttgaact cgccttttcc gctttcatca agcgttacgc
      361 tggcaatggc cgaggtggta aacataggcg gcgtttcgcc cggacataac tcggcgacca
      421 gatacccaac taacggtaag tgggagataa ccaacaccga agcaacacct tcattggtca
      481 acgcctgcaa ataggcactg accagaccaa catcgccgca gggcgttaac tccggcagaa
      541 cttctgcact ggaaggcagg ttcagacaat ccccaacttc ctccagtgtt tgctcggctc
      601 gcaggaacgg gctcaccaga acgcgttcga tttccacttt ttgacctttc agccagttcg
      661 ccatcaggcg agattcgtca caaccgttag tggtcagagg acgaaccgaa tcactggcgg
      721 catcgagggc tgcgtcgccg tgacgcatga taaaaacttg catattgcac cgcttttgtt
      781 aaccagattc gcccgttttc tttcatctga aaccagagaa agaaaacggt ggccggcatt
      841 gtgccttatc cattcaccga atgaaacgct gttttttacc tcaatggcgt aagtatagtc
      901 aatatgcgtt tacattttga ccaacaatcc gccattcagc gcggattcat atagctttga
      961 ccttcttatt gcaggtcagt tgcagttgtt ttccaaaaac tttccccacg cgcacccatc
     1021 tcgactaaac gctcgcaagg ggtaaaacgt gaaccatact gcgtggccag tcgttgcatt
     1081 attgcaacca cttcgcccgc gccgagagaa tcgatatagc gaaacggtcc accgagaaat
     1141 ggcggaaaac caatgccaaa taccgcacca atatccccgt cacgcacgct acggataacc
     1201 tgctcatcaa cacaacgtac agcttcattc agcatcaaca tcacacaccg ttcagcaacc
     1261 tgcggtgcgg agagtcgccc ctgcccttgt gcgccaatga gcgggtaaat ggcaggatcg
     1321 acctgttttt tgcttttacg ccctttctga ccataaagat agaaacctcg accatttttt
     1381 ctgcctttgc gatcgtcgtt caaaattgaa gaaacaacat ttgcaggcgc gctaaaacgt
     1441 tctccataag cggcttccag tacaggaata attttagtcc cggtgtcgat tcctacctca
     1501 tccaaaagtt ggattggacc taccggaaaa ccaaatttca ctagcgcggc atcaatgtgc
     1561 gctacccgtt caccttcggt cagcatgcgg atagcttcat taatgtaagg cgctaagatg
     1621 cgattgacgt aaaacccggc tttgtcacgc acgacaatgg gcgttttgcc ctgttttttc
     1681 gccagtttta ccgtagtggc gatggtttgt gctgatgtac ccgcatgagg aataatctcc
     1741 accagcggca ttttttccac cggactgaag aaatgcaggc cgataacttg ctcaggtcgc
     1801 gtggcgtgag cggcgatatc accaatcggt aaagatgacg tattcgaagc aaagatggta
     1861 tgagcggcgc aattttgctc aacttccgcc accatctgtt gtttcaattc gagattttca
     1921 aacaccgctt caataatcag atcgcgatgg gcaaagccgc gatagtccgt cgttccggag
     1981 attaatgcca gctgtttgtc acgttcgctg gctttgagat gacgacggcg aactttgccc
     2041 tccagctgat cccaactgta cttcagcgca tgatttatgc cctgcgggtt gatatcttta
     2101 attctgaccg gaatccccgc tttacaagca gtgacataag caataccgcc gcccatcaag
     2161 ccaccaccta aaatccccac gctgtttaat ggcgcaggcg gcgcatcact gccgggatct
     2221 ttcttcactt ccgtactggc aaaaaagata ctacgcaaag cttgcgattg tggcgtcatc
     2281 gccagttcgc caaacgcccg tgcttcagcg tcatagccac tgctggtgcc ctgcgctaat
     2341 cccgtttcaa caacctccag gatgcgttct gtcgccggat aattgccttg agttttgtgt
     2401 tctgttttct tgccgaccat tttgaacagc agcgcacgac ctaacggccc cgccagaata
     2461 cgctcgcgta caggtagagg gcgggaagat gggcgatcct tctttgccag ctcaacagcg
     2521 gcttccagca gaatggagtg cggaacaaca tcatccacca gccccagctt taatgcctgt
     2581 ttcgcccgaa gttgttttcc ggtgaggatc atctctaatg ctgtgctgac gcctatcaga
     2641 cgcggtaaac gctgggtgcc gcctgaaccg ggtaacaatc caagttgtac ttcaggcaaa
     2701 ccgagcaccg ttttaggatc gtcagtacaa acgcgaccgt ggcacgccag cgccaactcc
     2761 agcccaccac ccaggcaagc gccatgaata gccgcgataa ccggaatggg caaagcatga
     2821 atctccgcca tcaactgttg gccctgccgc gccagagctt ccgcttcttg cgccgttttg
     2881 caattgccga tcatgttgat gtctgcgcca gcaatgaagt tgtccggttt agcggagaca
     2941 aacaccacgc ctcgcaactc tttgttttca cggagttgct taataatggc gcgcacctgc
     3001 gaggcaaact ccgccttcag ggtattcatt ttctcacccg gtacgtcgat ggtgatgata
     3061 gcaatgttgt ccagacgaac attaagagta aacgctgatg tcatttccat tattccgcct
     3121 ccagaaccat tgccgcgcca agcccaccgg cagcacaggc ggtaactaaa ccaaatccac
     3181 cgccgcgacg gcgaagttca tgcaatgtct gggtaatcat ccgcgcgccg gtcgccgcga
     3241 agggatgccc gtaagcaatc gaaccgccaa gcacgttaaa tttgctatcg tccacttcgc
     3301 cagtggcatg tgcacgcccc agcgcttcac gagcaaaacg ttcactaccc agtagctgaa
     3361 tattggccaa cgtctgggcg gcaaaggctt cgtgcatatc aatcaatgtc agatctgcca
     3421 ttgtcaaacc ggcacgctcc agcgccagcg gtgttgacca ggctggaccg agcaacatgt
     3481 cctgccagac atcgatagcg gtaaatgcgt agctgcgcag atatcccagc ggtaccagcc
     3541 ctaattcttt cgcccgggat tcagtcatca ggatcaccgc cgctgcgcca tcggtcagcg
     3601 gcgtactgtt cgccgccgtt accgttccgt gtttacgatc aaacgccgga cgcagctttg
     3661 cgaaatcagc aagcgaggaa ttaccgcgaa tattgttgtc ttccgcgagc agttgtttat
     3721 aaggagggat aaaggcagtc atcacctctt ctttgagttt tccgtctgac catgcctgag
     3781 cggcacgctg atgcgaacgg tgcgctaatg catcttgctg ttctcgggtg atgccgtagg
     3841 ttttcgccat ttgctctgca gtgtcgccca tccgcaagcc ggtagaatat tctgctaccg
     3901 caggtggtac gggcattaag tcgcgcaaac gcaggcgaga gaagagtttc agacgctggc
     3961 tcattgtgcg ggctttgttg acatcaacca gcacgcgcgc cagtttttta ctgacgccaa
     4021 ttggcaatac cgaagaggaa tctgccccac cggcaatccc cgctcggata gtccccgcca
     4081 tcaggctttc tacgacgttt gcaaccgcct ggaaactggt agcgcaagcg cggctgacgc
     4141 tgtaagcatc ggtatgcaca ttcattccag taccgagaac aatttcacgc gcaatgttgg
     4201 gagcttcagg catttgtacg acctgaccaa agaccagttg ttcaatcact tcggcgggga
     4261 tctcgctgcg tgccagcagt tcgcctacca ccatcttccc taaatcaacc gcgggaatgc
     4321 catgaaaagc cgtcgcctgg cgggcaaaag gcgtacgtaa accgctaaca atggcgatac
     4381 gatcgccctg gcgggtaacc agcggtaaaa cctgacccat aacactcccc tgtaaaaaaa
     4441 taaataaagt ggtctgacct gatcatagtc ttaaccattt ttttacattt agccaagcgg
     4501 agaaaaggga aattgggagc tatgacacag tgaagacagg agaaagaaaa gaaaacgccc
     4561 ctacctgttg ctggcaggag cgtacttatc cgagaggaat taacgcagac ccagctggaa
     4621 gatcagcatt tcagcttcgc aggcaaaagt gaaatcgata tccaggcgca caccatcaga
     4681 ctcttcagtg aaagtcgggg agattttgca cggttcggat tccacgctac gggctttctc
     4741 agtcagcgcc gccagcgttt gttctgcttc tgcgcggttt gcaaacacgc ggctgtaaga
     4801 cgctgtgcag tcggagttgt ccataatggt gccaacatcc atacagcagc aaaccggggt
     4861 ttcatcagca ctacatttac tcatcgttga tttcctctgt atgtgcaccc aaggtgccag
     4921 ataaacgttg tggatatttt acgcttccgg aaagtgctgc tccagttgtt aattctgcaa
     4981 aatcggataa gtgaccgaaa tcacacttaa aaatgatcta aaacaaaatt cacccgaatc
     5041 catgagtgcg ccacctccaa attttgccag ctggatcgcg tttcttagat catatttgaa
     5101 aaaagataga aacatacttg caacattcca gctggtccga cctatactct cgccactggt
     5161 ctgatttcta agatgtacct cagaccctac acttcgcgct cctgttacag cacgtaacat
     5221 agtttgtata aaaataaatc attgaggtta tggtcatgag ccagaaaacc ctgtttacaa
     5281 agtctgctct cgcagtcgca gtggcactta tctccaccca ggcctggtcg gcaggctttc
     5341 agttaaacga attttcttcc tctggcctgg gccgggctta ttcaggggaa ggcgcaattg
     5401 ccgatgatgc aggtaacgtc agccgtaacc ccgcattgat taccatgttt gaccgcccga
     5461 cattttctgc gggtgcggtt tatattgacc cggatgtaaa tatcagcgga acgtctccat
     5521 ctggtcgtag cctgaaagcc gataacatcg cgcctacggc atgggttccg aacatgcact
     5581 ttgttgcacc gattaacgac caatttggtt ggggcgcttc tattacctct aactatggcc
     5641 tggcaacaga gtttaacgat acttatgcag gcggctctgt cgggggtaca accgaccttg
     5701 aaaccatgaa cctgaactta agcggtgcgt atcgcttaaa taatgcatgg agctttggtc
     5761 ttggtttcaa cgccgtctac gctcgcgcga aaattgaacg tttcgcaggc gatctggggc
     5821 agctggttgc tggtcagatt atgcaatctc ctgccgggaa gactcctcaa gggcaagcat
     5881 tggcagctac cgccaacggt atcgacagta ataccaaaat cgctcatctg aacggcaacc
     5941 agtggggctt tggatggaac gccggtatcc tgtatgaact ggataaaaat aaccgctatg
     6001 cactgaccta ccgttctgaa gtgaaaattg acttcaaagg taactacagc agcgatctta
     6061 atcgtgtgtt taataactac ggtttgccaa ttcctaccgc cacaggtggc gcaacgcaat
     6121 cgggttatct gacgctgaac ctgcctgaaa tgtgggaagt gtcgggttat aaccgtgttg
     6181 atccgcagtg ggcgattcac tatagcctgg cttacaccag ctggagtcag ttccagcagc
     6241 tgaaagcgac ctcaaccagt ggcgacacgc tgttccagaa acatgaaggc tttaaagatg
     6301 cttaccgcat cgcgttgggt accacttatt actacgatga taactggacc ttccgtaccg
     6361 gtatcgcctt tgatgacagc ccagttccgg cacagaatcg ttctatctcc attccggacc
     6421 aggaccgttt ctggctgagt gcaggtacga cttacgcgtt taataaagat gcttcagtcg
     6481 acgttggtgt ttcttatatg cacggtcaga gcgtgaaaat taacgaaggc ccataccagt
     6541 tcgagtctga aggtaaagcc tggctgttcg gtactaactt taactacgcg ttctgataac
     6601 gcgttcgcct ggataaagtc acctgctatg caggtgactt taactccccc acttcaccga
     6661 aagtagtttc ctcattgctt gcctcctgag ttttgcaaac agcctgatgg cagctagccc
     6721 tttcaaatca gtaagtggta tccacagaca aacctgaagg gaaacgcata ttttcaggcg
     6781 ttctgctcgt ccttctcaaa gagtttactt ttctgaattt ccaggatact caaccccctg
     6841 gctattgtgc gctcatacac gcaaattaaa gataggttct aaataaatga gcgttttttg
     6901 atagtctatt tcattaggta atatatattt gtaacaaatc aatcaacatg gaataaaatc
     6961 atgctatcat ctatttcaat caacaatacc agcgcagctt acccagaatc catcaatgaa
     7021 aataacaatg atgaagttaa tggattagta caagagttca aaagcctttt taatggtaag
     7081 gaaggaataa gcacctgtat taaacatcta cttgagctta taaaaaacgc catacgagga
     7141 aacgacgatc ctgatagaat aaatatcaat aattcttcag ttacttatat taatattggt
     7201 tccaatgata cagaccatat tactattggt atcgacaacc aagaaccaat aaaattacct
     7261 gcgagttata aagacaaaga actcgtccgt actatcatta atgacaacat agttgagaag
     7321 actcatgatg atataaataa tgctgaattt actgatggtg gtattgatga tcgtagtgat
     7381 attggcattg actccaatga tacagacgat attgatattg ataatcaaga accaataaaa
     7441 tcacccgcga acgatgaaaa caaaaaagtc ggccaaacca tcatcaatga aaacatagta
     7501 gagaatactc atgatatcaa taacaagaa
//
LOCUS       DAZFDC010000178         7510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFDC010000178 DAZFDC010000000
VERSION     DAZFDC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.178"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1013
                     /locus_tag="ACHJ48_004334"
     CDS             <1..1013
                     /locus_tag="ACHJ48_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO4709762.1"
                     /translation="RRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITRIVSA
                     GAPADLLLLAVAPEKMVGFSSFDFARQALIPLSEHIRQLPRLGRLAGRASTLSLEGLM
                     ALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLGEEHR
                     AAEQANLASRFVGEAQAFAASPAANLRFYAARGPRGLETGLKGSLHTEAAELLGLHNV
                     AQIADRQGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWHGVKAVAEQRILFLSG
                     LPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADYQKLV
                     AT"
     gene            1013..1993
                     /locus_tag="ACHJ48_004335"
     CDS             1013..1993
                     /locus_tag="ACHJ48_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO4709763.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFVLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            1990..2748
                     /locus_tag="ACHJ48_004336"
     CDS             1990..2748
                     /locus_tag="ACHJ48_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO4709764.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2758..3570
                     /locus_tag="ACHJ48_004337"
                     /pseudo
     CDS             2758..3570
                     /locus_tag="ACHJ48_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            3567..4421
                     /gene="modD"
                     /locus_tag="ACHJ48_004338"
     CDS             3567..4421
                     /gene="modD"
                     /locus_tag="ACHJ48_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO4709765.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4447..6417
                     /locus_tag="ACHJ48_004339"
     CDS             4447..6417
                     /locus_tag="ACHJ48_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4709766.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSELGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLTFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRKRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFVEEGRNFWAGVEYTF"
     gene            complement(6467..6721)
                     /locus_tag="ACHJ48_004340"
     CDS             complement(6467..6721)
                     /locus_tag="ACHJ48_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO4709767.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            6922..>7510
                     /locus_tag="ACHJ48_004341"
     CDS             6922..>7510
                     /locus_tag="ACHJ48_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO4709768.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWIGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKV"
BASE COUNT         1818 a         1994 c         1922 g         1776 t
ORIGIN      
        1 ccagacggag gtttacacag attcttgcgt cgacgttgtt cctgcatcat ctgccttctt
       61 ttgcacaatc agtcaaattc tgggcctcac tgacgcttcc cgaagcccaa aacattacac
      121 gcatcgtcag cgcaggtgcg cccgccgatt tgctattgct ggctgtcgcg ccagaaaaaa
      181 tggtcggttt ttcctctttt gattttgccc gtcaggcatt aattccattg tcagagcaca
      241 ttcgccagtt acccaggctg ggacgactcg ccgggcgcgc cagcacactc tcgctggaag
      301 ggcttatggc gttacatccc gatttggtcg ttgattgcgg caatacggat gaaacctgga
      361 tctcccaggc acggcaggtt agcgaacaga cacaaatacc ctggttattg cttaacggga
      421 aactggcgca atcagcagaa cagttaacaa cgcttggcaa aacgttaggc gaagagcatc
      481 gcgccgcaga acaagccaat ctcgccagcc gcttcgttgg tgaagctcag gcattcgccg
      541 cctcacccgc cgctaacctc cgcttttatg ctgcgcgcgg ccctcgaggg ctggagacgg
      601 gcttaaaggg ttcgttgcat accgaggcgg cggaattatt aggtttgcac aacgtcgcgc
      661 aaatagccga tcgccaaggc ctgacacagg tttccatgga aaatctcctg cgctggcagc
      721 cggatattat tctggttcag gaggccgtta ctgcagattt tattcgtcgt gatcctctct
      781 ggcatggcgt gaaagccgtt gcagaacaac gcatcttatt tttaagtggc ttgccctttg
      841 gctggctgga tgccccaccg ggaatcaacc gtcttctggg attacgcaga cttcacgcct
      901 ggctggatcc cgccatcaat cgccagttta aaagtgacat gcagcattac gcccaactgt
      961 tctggcattg ttcactcagt gacgccgact atcaaaaatt ggtggcgacc taatgagaat
     1021 cgtaaacgga tgtattttgc tgctggcagc tatcagtatc acttttgccg ccgtaagtgg
     1081 ggcttatcat ctcgatatgc agcaactgct ggcgctaatt cttcgtcaag aaaatgttcc
     1141 tgtgcaggaa caaattgtct tctggcaaat ccgcgtgccg cgtattcttg ctgcgctgtt
     1201 actgggcgcg gcgctggcag gagccggaac cacctatcag ggaatgttgc gtaatccctt
     1261 agtttcaccg gatattttag gcgtctccgc cggtgccgga cttggtgcct gcacggcaat
     1321 tctgtgggga ttatccattg tttacatcca gctgtacgca ttttgtggcg ggctgatggt
     1381 ggtcgcgggc gtctggttga ttacgcgtcg ggtgacccgc cacgatccga tcttaaccct
     1441 ggtgctggta ggcatcgcgt taggaacgtt gtgcggagcg gggatatcgc taattaaaac
     1501 gctggccgat ccttacaccc agttaccgtc aattaccttc tggttgctgg ggggactgtc
     1561 caccgtgacc ttacgcgatc tctgctatgc tgcaccgatc attctcatag gctccttgcc
     1621 cctgtttttt ctccgctggc gcatgaattt actcacgctt tccgacgacg aagcccgttc
     1681 gctgggactg aacgtgacgc gtctgcgctt tgtattgatt gtctgcgcca cactaatcac
     1741 cgccagtacg gtagcaatag cgggcataat tggttgggtc ggcctggtcg ttccgcacat
     1801 tgcccgcttg ttgaccggtc ataaccatca gcagctgttg cccatggcaa tgtgtactgg
     1861 cgcgattctg ctcctgctta ccgatacgct ggcacgcagc attggaacaa cagaaatccc
     1921 acttggcatt ctgaccgctt ttgttggcgc accgtttttc ctgcttttgt tgttacgcgg
     1981 aggtcgccag tgactttgct tgccgtccgt catgcgtctc tgggttactc ccgccatccg
     2041 gtgctacgcg acgtgtcctt caccctgtcg caaggaacaa tttgttgtct gttaggagcc
     2101 aatggttgcg gcaaaaccac actcatgcgc tccattcttg gcgttatacc gttattaaaa
     2161 ggcgaggtct tactggatag cctccctgtg cagacactca gccatcgcca gcgcgcgcag
     2221 gctattgcct gggtgccgca ggcgcacgac ggtatttttg cttttagtgt gctggatatg
     2281 gtgttgatgg gcctggcccc caccataggc gctttttccg ttcccggtaa gcaagaacgc
     2341 ctgaaagcca cggagcaact ggaaaagctg ggcattttgc atctggcggc acgacgctgg
     2401 aatacgctta gcggcggtga acgtcagctg gctttaatcg cccgtgcact cgtccagcaa
     2461 ccgcgcttgt tgttactgga tgagcctgcc tccagcctcg atttcggtca tcagatccaa
     2521 ctactggaca cgctggcaca gctcaaaaat aacggcatga caatgttaat gtcgactcat
     2581 catccgctcc atgccaacgc cattgccgac agcattatcc aggtagagcc agatggacga
     2641 gtaacacaag gcttaccgac cgaacagtta acaacaaaca aactggctgc cctgtatcgg
     2701 gtttctgccg accagattca tcatcacctc tctgcaatta gccactaagg aaaaatcatg
     2761 ctcattgatg atattgattt tgcagacctt tatctgcagc aactcaagct ggcccaccgc
     2821 acagaaaaaa cgccggatca ctgggatcaa cgggcggaaa aaatggcgga aaattgcgcc
     2881 tcgcctacgg acagctacct gcaacaactg atcgctaaaa ttgacctaca aggcgctcag
     2941 accttattcg atatgggctg cggaccgggt accgtatcgc tggcgctggc tgacaaactc
     3001 acgacgattt atggcgttga ttacagtcag gggatgctga acgttgccgc ccgtcgcgcc
     3061 gccgcgctga aggcagacaa tgtccacttg atccagcgcg cctgggaaga ggactggagt
     3121 gacctgccac gctgcgatat tgccgtggct tcccgctcca ctctggtggc tgatatgcgc
     3181 caggcaatga gcaaactgaa taatcaggca cggcttcgcg tctatacgac tcatctggtc
     3241 agcacctcgt tcgtctctcc agccattcag cgggcggctg gtcgggaagt gatcgaatta
     3301 cccaactata tctttgcgct caacgtcctg tatcagatgg gaatttatgc ccacgttgat
     3361 tttatccgtg gacaaaattg ccaacaggat aacagcacct aggaacgttt tgagcagaac
     3421 gtcagctgga gtcttggcgc gcttaacgac gatgagcgtg agcggttata tcgctggtat
     3481 caacaacaag atgctcgtgc cctcgcccct gccagccgcg actgggcgct catctggtgg
     3541 gatagcgtac cgcaggaggc actgcgatga ttttcctttc tcaggcacaa atcgatgcgt
     3601 tacttctgga agatatccag ggcggtgacc tgaccacgag agcattaaat attggacacc
     3661 agcatggcta tatagagttt tttctccgtc agggcggctg cgtcagcggc gtttctgtcg
     3721 cgtgtaagat gttaactacg ctgggattaa ctattgatga cgcggtcagc gacggttcac
     3781 aagcgaacgc gggtcagcgg ctaatccgtg cgcagggtaa tgccgcagca cttcatcagg
     3841 gatggaaggc aatccagaat gtgctggagt ggagttgcgg tgtttctgat tatctcgatc
     3901 aaatgctggc gttacttcgt gaacgttatc ctgatggcaa tatcgcctgc acccgaaaag
     3961 caattccggg cacccgctta ctggcctcgc aggcaattct ggctgccgga ggactgattc
     4021 atcgtgccgg atgtgcggaa accatattat tgtttgccaa ccaccgccat tttcttcatg
     4081 acaatcagga ctggtcaggc gcaatcaatc agttacgtcg ccacgcaccc gagaagaaaa
     4141 ttgttgttga agccgacacg ccgaaagagg caatcgccgc gttacgcgcg caaccagacg
     4201 tacttcagct cgacaaattt agtccgcaac aggcaactga aattgcgcaa atagcgccgt
     4261 cgctggctcc ccactgcacg ctggctctga ccggcggaat taatctgacg acactcaaaa
     4321 attacctcga ctgcggcatt cgcctcttta ttacctccgc gccttattac gcggcaccgg
     4381 ctgacattaa agtcagtctg caacccgcag cctctattta attctttgtt aaacggaatt
     4441 tattctatga gactcaaaaa acattatctc tgcacagcat tatcacttgc ctttacccag
     4501 caggccgtag cggctcagga gagcgactcg ctgaccgtat ggtccagtcc ggtatcatcg
     4561 acgacgacca ccgttctcga tcaacccacc atgaaggccc tggataaaca gaatgtcgct
     4621 caggcattaa gtgtcgtccc cggcgtggtg ctgcaaaagt caggcagccg caacgaagaa
     4681 caggtaaaag ttcgtggctt tgatagtcgg caggtgccgg tctatttcga cggtgtgccc
     4741 atttatgttc cctatgacgg caacctcgat ctggcgcgga ttctgaccaa caatctgggg
     4801 gcagtcgaag tttccaaagg gtattcgtcg ctgcttcagg gacctaatca gatgggcgga
     4861 gccattaata tcaccaccca gaagccaaca aaacctctgg aagcaagtct gggatatcgc
     4921 cagggatgga gccgtagcca ggacaatgcc tatgatatgc atgcttcatt tgccgccagc
     4981 agcgaactgg ggtatttgca agtcagcggt agccagctaa agcaggattt tctcggcctg
     5041 ccgcatggtg taaataatga tattgcaggc aaacacggca agatgattaa ttcatcggct
     5101 gatgataaac gcggcattgt gaagctcgga tttacaccac gtgaaaacga tgaatacaca
     5161 ctgacttaca ttaagcagga tggtgaaaaa gataacccgc catacagcgg aaatagtggt
     5221 caaaaatcac gctactggca gtggccagag tatgacaaag aaagttttta ttatcaggga
     5281 acgacccaac taaacgatcg ttttaccctg aaaagtcggc tgtatcgcga cacctttgaa
     5341 aatacgctga tgatgtacaa ctcgctggct gatttgaaaa ataaaaaagg cagctacagc
     5401 cattattccg attacagcga cggtgccgga ttacaactgg cagccgatgt gcgtgaaaac
     5461 gatctgctga cgtttgccgt taactggaaa gatgacgtac accgggaaaa aggtgcgccg
     5521 cacgccgctt acgatcgcta tgaagatcgt acctggtcgc tcgccagtga atatcaatgg
     5581 gctgctgccg ataatgtcga tgttgtggct ggaatcagct atgactggcg cgatagcgta
     5641 gaagcgaaaa aacacgagaa agatggcagc atcacccatt atgacgacaa caatcagtca
     5701 gcttttaact ggcaggtgat ggggaaatac cactttgtca atgaagacac gctggcgctt
     5761 tcgtactatg accgcaaacg ctttccgacg ctgaaagaac gctataccac atccaaacct
     5821 gcgtataacc agatagcgat tgttaacccg cagctcaagc cggaacgcgc tcgcggggtg
     5881 gatttaacct ggaatggtgc cttcacgcac gactggggat ttgaggtcag cgtttactat
     5941 aaccgggtga gtgatgccat cctctcgcac aatatcgatg ccgataccat tcaaaatcag
     6001 aacagcggca cggtggatta cagcggtctg gatgccggta ttaaggggaa aatcagcaat
     6061 atactggatg taggattgag ctacgccctg atccacgctg acgccaaacg taaagacatc
     6121 ggcaagataa ccgatctgcc aacgcagaca atgaccgcat ggatgactct caaaccgtgg
     6181 gagccgttaa gcgtaacgct gtcggaagag gcgcgttcct ccagctacag taacagtgac
     6241 ggttcacaaa aagccgccgg ttttgcggtg acccacattc gagccgatta caccttaggt
     6301 catggcttca gcgtcaatgc gtcggtcaat aacctgtttg atacccaata tgcctacagt
     6361 gaagggtttg ttgaagaagg tcgcaatttc tgggcaggtg tcgagtatac gttctgatgt
     6421 gaaagcggat agcggcatct gtatcgctat ccgccttatg aaaatcttat tctcttcgca
     6481 ggaggcggaa tacgcccaga acgagaatag ctcccaccac cgccaccagg aagctgtgca
     6541 aattaaaacc gctgatggag ccgccaatgc caaacatggt cgccagccag ccgccgacca
     6601 ccgcaccgac tatcccgaga atacaggtca ggaaaaatcc accaccatca cgccccggca
     6661 tgattagctt ggcgataatg ccggctatca ggccaaaaat aatccaggca ataattccca
     6721 tgttcaggcc ctcgtttcga tgaatcggac gcaagaaatg cttacgtcaa cgctctaagt
     6781 ataggcaagg gcgggatcga tgctgggcgt atgtcggctg atttaagaac aacatgtaaa
     6841 aaatattcac gcatttgtat taagttttgt taactgtgac cgataaacca aagacagttt
     6901 gtcagtcagg agtttttccg cgtgagtcat taccatgagc agttcctgaa acaaaatccg
     6961 ttagccgtcc tgggcgtgtt acgcgatttg cacaaagccg caattccttt gcgtctcagt
     7021 tggattggcg ggcagctgat cagcaaaata ctggtaataa ccccggataa actggtgctg
     7081 gatttcggca gtcaagccga agacaacatc gccgtgctaa aggcacagca cattaccatt
     7141 accgccgaaa ctcagggtgc gaaagtcgag tttactgttg aacaactaca gcagagtgaa
     7201 tacttgcagc ttccggcatt tattaccgta ccgcctccca ccttatggtt tgtacaacga
     7261 cgccgatatt tccgcatctc cgccccactc catccgcctt atttttgcca gaccaaactg
     7321 gcggataaca gtacgttacg tttccgcctg tatgatttgt cgttaggcgg catgggcgca
     7381 ttactggaaa cagcaaaacc tgccgggttg cacgaaggta tgcgcttcgc tcagattgaa
     7441 gtcaacatgg ggcaatgggg tgtttttcac tttgacgccc agttaatctc catcagcgag
     7501 cgcaaagtga
//
LOCUS       DAZFDC010000179         7446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFDC010000179 DAZFDC010000000
VERSION     DAZFDC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.179"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..685)
                     /locus_tag="ACHJ48_004342"
     CDS             complement(<1..685)
                     /locus_tag="ACHJ48_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO4709769.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNFRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGK"
     gene            complement(696..1697)
                     /gene="ltaE"
                     /locus_tag="ACHJ48_004343"
     CDS             complement(696..1697)
                     /gene="ltaE"
                     /locus_tag="ACHJ48_004343"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO4709770.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGSELKELTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1734..3452)
                     /gene="poxB"
                     /locus_tag="ACHJ48_004344"
     CDS             complement(1734..3452)
                     /gene="poxB"
                     /locus_tag="ACHJ48_004344"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO4709771.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3585..4553)
                     /gene="hcr"
                     /locus_tag="ACHJ48_004345"
     CDS             complement(3585..4553)
                     /gene="hcr"
                     /locus_tag="ACHJ48_004345"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO4709772.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4565..6217)
                     /gene="hcp"
                     /locus_tag="ACHJ48_004346"
                     /gene_synonym="priS"
     CDS             complement(4565..6217)
                     /gene="hcp"
                     /locus_tag="ACHJ48_004346"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO4709773.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6361..7260)
                     /gene="lysO"
                     /locus_tag="ACHJ48_004347"
     CDS             complement(6361..7260)
                     /gene="lysO"
                     /locus_tag="ACHJ48_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO4709774.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1792 a         2013 c         1966 g         1675 t
ORIGIN      
        1 gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc
       61 cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc accagatagt
      121 gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta
      181 acactggcag gttgtagacc atatcgcgca tgacttcgaa cgctgctgaa cctgcaccaa
      241 cgattattcc ggcacgcaat tcggtcactg gtacattcgc ttcacgaaga atgtccgccg
      301 tagcctgacg cgcgcgcaga tggtccgact gctcatgtgg cggggcctgc aacgaactga
      361 gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggaagttg agagccacct
      421 ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct atgcaccaga aaatagaccg
      481 tatcgatatc ctgtaacagg gccggaaggt tatccggcca gctgagatcg actttatggc
      541 aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga
      601 tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag ccactggcac
      661 caagaactaa aatgcgttgc ggcacgtctc cctccttaac gcgccaggaa tgcgcgccag
      721 tgggcggcga cttctgccag ttgttcgcgc gagacgtcaa gatgggtcac taggcggaca
      781 atcggcgagg cgttaatcag tacgtttctc gctttcatgt attcgcctaa cgcggcagca
      841 ttttcttcgc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac atccgcgcct
      901 gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg
      961 acgttatttt tcagcgcata catcccggcg gcagccagaa tgccggactg gcgcatcccg
     1021 ccacctgtca ttttccgcca gcgaatggca cgtttaatgt aatcacgatt accgacgagt
     1081 aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat
     1141 tgcgtgagct ctttcagttc gctgccgtaa gccaccacgg cattaaagat gcgcgcaccg
     1201 tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcatcttt aaggtattca
     1261 cgcggcaaca ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa
     1321 tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca
     1381 gccgcgtcga tgggttgcgg ttgaatactg cccagcaccg ccgcgccacc ggcttcaaac
     1441 agatagttat gcgcggcctg accgacaata tactcttcgc cgcgttcgca gtgactgagc
     1501 agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa
     1561 agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc
     1621 ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt tacggtatca
     1681 ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt
     1741 agccagttcg ttttcgccag ttcgatcact tcatcaccgc gtccgctgat gattgcgcgc
     1801 agcatataca ggctgaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaac
     1861 tcttctttgg cgaccaccac atccaccaat accggaccgt cgatggagaa ggcgcgttgc
     1921 agggcttcat cgatttcaga ggctttttct acacgaatac ccgtaattcc gcacgcttcg
     1981 gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta gcctccggct
     2041 ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat tttcaccggc
     2101 agtttcatct gcactaccga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg
     2161 cacatggcga ccacctgacg ttccggctcg gttgcctgcg cgcccagcgc ctgcggcatg
     2221 gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttc
     2281 agataacgcg ccgcccacac cgttggcgtg ccgacgtcac aggtaaagat ggcgtcatcg
     2341 gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt
     2401 ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc tttatccaga
     2461 aacttgcgat cggctttttc ttccaccaat ggcagcaatg cacgcagggt cgacttgata
     2521 tcgccgacca gcgccatatc caccttgctg tgtgcgccga tgctggctgg gttgatatca
     2581 atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg agtaccgagt
     2641 agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc gattaacccg
     2701 gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg tagggcatga
     2761 acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag
     2821 ccgctgccac acatcagagc gatattgctg gaataacgca gcagttgcgc cagtttgcgt
     2881 aactcttctt cttccggcgt cacgactggc tgtggcgcat gataccagtg ggtagttgcc
     2941 ccttctggcg caggttttaa cgccacgtcg cccggtaaca cgacaacgga aacgccacgg
     3001 ttaagcaccg ctttgcgcat ggcaatcgcc agcacttgtg ggatctgctc cgggctggaa
     3061 accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa
     3121 tagccgctgc caatttcgct ggaaggaata tgagcggcaa tcgccagtac cggaacgtga
     3181 ttgcggtggc aatcgaacag gccgttgatt aagtgcaggt tgccggggcc gcacgatcca
     3241 gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa cgccgccact
     3301 tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact gtcgctaaga
     3361 ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga ttcgagtgtt
     3421 ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg
     3481 gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac
     3541 ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc gagaaccaaa
     3601 tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc
     3661 agcgtcattg tactgctcac cgtatattca ccggaaacca ctttcgtctt acagcagccg
     3721 caaacacccg cgcggcaggc ggcgacaacc ggaacgttat tgctttccag cgcctccagc
     3781 agcgtggtgc caacaggagc gtaaaattct tgcgccggtt gcagtttggt gaatttcaga
     3841 ccgctggtcg cagcttccgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg
     3901 ccgagtgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc gcaggtcatc
     3961 acggtacgtg aagctaagtc aggaacgcga gtcaggagtt cgcgagtgag acgaccagcg
     4021 ataaagcctt cggtaacgtt attttccgcc accagcgtta ccggatagtt acgccactca
     4081 tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggcctgt
     4141 ggacggttct tcgccagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgca
     4201 gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtct
     4261 gaaagccaga gataatcacc gcgttttaca tcgcgggtca gccactggga gccgacaccg
     4321 tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt ggaggaaatg
     4381 gtgtaagcac gcagcgtttc cgctgagtta cgcacgctga ccagtgcata ttgcccggcg
     4441 cggtatgggt agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc
     4501 ttaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt catcgacaaa
     4561 ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc
     4621 cgaatttctc gttcagcacc gccagcagat ctggtgtcag gaaaccaggt gcagtcgggc
     4681 cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca atcgctttct
     4741 gttcaaacca ggagagcacc agcgacagcg gcagatcgtt gacaccgcag cccagttttt
     4801 ctgccagcgt aaccgccaga ataattgctg agtaagcatc gttacactga ccggcatcca
     4861 ccagacgagg cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac
     4921 aggcgagagt caggatcagg cagtcatctg gcacgctggt ggcgaaatcg gtgaagtagt
     4981 gacgttcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac
     5041 ggctcaccag atcaattagc gtatccgctg cgccaagcag cgtctggcgg ccaaagccca
     5101 cggtgatcag gtgcggaatt tcgctgtatg ggaagcctgc catttgttgc gcctgggcaa
     5161 tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca acgatgctgc
     5221 gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca
     5281 tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag ccgctgccgt
     5341 agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca ggcagcattt
     5401 cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat
     5461 ctttgagatc gtgaccggaa atcaggatgc atttacccgc cgtcgctttg acgttgacct
     5521 gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt
     5581 tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc
     5641 aggtccccag ccacgccatg attttatgat actgggcata aatatcgttg tcgtattgac
     5701 cgagaacgtg tgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca
     5761 gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg
     5821 ctgcctgacg ttgcagttcg ccgagatcgt cgctcaccag ttgcaggtca gccatcgggt
     5881 tatcgacgcg ggcgttggca tctacagcca ggcattgtgc tttcagcgcc tcgcgcaagg
     5941 caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg
     6001 ttgagaaaaa ggcacgtggc gcgaagctgt cgacatcgtg gttgatgatg ccgtattcac
     6061 gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt
     6121 cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg
     6181 gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat
     6241 ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat ttttcatgca
     6301 actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc cagagaggta
     6361 ttacgcggag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac
     6421 aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc
     6481 catcgacgtc gcaccgcata agcccagtgc ggtagagcgg ctgcggcgaa tcagcccagg
     6541 gatcaacata attgcaatca gctcacgggc cagatcatta aaaaacgccg cgctcccgat
     6601 taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccaga
     6661 ggccattgcc agcgcggtat tgatgggtag atcaagaata aaggcgttaa ttaaaccacc
     6721 aattaatgaa ctggcaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat
     6781 ctgctttaag gtcatgccat tattgcgcaa ctgaataccg acgaggaaaa gtagcagaat
     6841 taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcaggccaat
     6901 ggcaaaacca atcactacta cgccgcacag ttttagcgac tcaagcgcca tcgcaatacg
     6961 cgacgggagt ttttcttgct gatggtggtt gcgccacggc agaccgcgct ccagccacat
     7021 aagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat
     7081 cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag
     7141 aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag cttgttggcg
     7201 aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacaacc cagaaaacat
     7261 gaaaaaccag cccttgcaaa gaaagaagtc gaaatgcgcg atgacacact actgaaagcg
     7321 gaaggacgag taaagttgca attaaaagga aatgttatgc atcagagggg gatttttaat
     7381 tctatatgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata taaagccact
     7441 ggcgca
//
LOCUS       DAZFDC010000180         7317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFDC010000180 DAZFDC010000000
VERSION     DAZFDC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.180"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            116..1744
                     /locus_tag="ACHJ48_004348"
                     /pseudo
     CDS             116..1744
                     /locus_tag="ACHJ48_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
     gene            complement(1744..1866)
                     /locus_tag="ACHJ48_004349"
                     /pseudo
     CDS             complement(1744..1866)
                     /locus_tag="ACHJ48_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2094..3164
                     /gene="dhaK"
                     /locus_tag="ACHJ48_004350"
     CDS             2094..3164
                     /gene="dhaK"
                     /locus_tag="ACHJ48_004350"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO4709775.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     GVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            3175..3807
                     /gene="dhaL"
                     /locus_tag="ACHJ48_004351"
     CDS             3175..3807
                     /gene="dhaL"
                     /locus_tag="ACHJ48_004351"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO4709776.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSCVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAEFAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            3818..5236
                     /gene="dhaM"
                     /locus_tag="ACHJ48_004352"
     CDS             3818..5236
                     /gene="dhaM"
                     /locus_tag="ACHJ48_004352"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO4709777.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSPL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSPVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            5556..7240
                     /gene="treA"
                     /locus_tag="ACHJ48_004353"
                     /pseudo
     CDS             5556..7240
                     /gene="treA"
                     /locus_tag="ACHJ48_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT         1932 a         1823 c         1844 g         1718 t
ORIGIN      
        1 agctcatcat gattgcacca tacaaatatc atgatgataa gaggatatta ttcattgact
       61 taaacaaaat gacaaaatcc atagacccat agcctgggta ctatatagga gtggtatggg
      121 catcaaacaa cacaatggga ataccaaagc cgatcgtctc gctgaattaa aaatccgttc
      181 gccctcaatt caactgataa aatttggcgc tattggtttg aatgcaattc tcttttcccc
      241 cctgctgata gctgctgata caggaagtca atatggcacc aatattacta ttaatgatgg
      301 tgacagaatt actggagata ccgccgatcc atcaggaaac ctctatggtg taatgacccc
      361 agcaggaaac acgcctggca atatcaacct gggtaatgat gtcaccgtca atgtcaacga
      421 cgcctctgga tatgcaaaag gaatcattat tcagggcaaa aacagctccc tgacagctaa
      481 ccgactcaca gtagatgttg ttggtcaaac ctctgccatc ggcattaatt taattggtga
      541 ctatacccat gctgacttag gcacaggcag caccattaag agtaacgatg acggcatcat
      601 tattgggcat agctcaacac taacagccac tcaattcacc attgaaaact cgaacggtat
      661 aggcctaacc atcaatgact atggcaccag tgtcgatctt ggaagcggaa gtaaaatcaa
      721 gaccgatgga agtacaggtg tttatatcgg tggtctcaac ggcaataacg ccaatggtgc
      781 tgcgcgtttt acggcgacag acctgacaat cgatgttcag ggctacagcg ccatggggat
      841 aaacgtacag aaaaactctg ttgtcgatct cggaacaaac agttccatta aaaccagtgg
      901 cgataatgca cacggcctct ggagctttgg ccaggttagc gcgaatgcac tcactgttga
      961 tgtaactgga gccgcggcca atggcgtcga agttcgtggt ggtacaacca ctatcggtgc
     1021 agatagccat atttcttccg cgcagggcgg tggtctcgtc accagtggtt cagacgcgac
     1081 aatcaatttt tctggcacgg cagcgcaacg aaacagcatc ttttccggcg gttcttatgg
     1141 tgcctcggcc cagacggcaa cggctgttat caacatgcaa aataccgata ttacggttga
     1201 tcgtaatggc agtctggcgc tgggtttgtg ggcgctcagc ggcggtagaa taaccggaga
     1261 cagtttggct atcaccggcg cggcaggagc cagggggatt tatgccatga ccaacagcca
     1321 gatcgacctc acgagcgatc tggtcattga tatgagtaca cccgaccaga tggccatcgc
     1381 aacgcaacat gacgatggtt atgccgccag ccgcatcaac gcctcgggtc gtatgcttat
     1441 caacggtagc gttctttcca aaggtgggct aatcaatctg gatatgcacc ctgggtcggt
     1501 ttggacaggt tcctccctca gcgataatgt caatggcggg aaactggacg ttgcaatgaa
     1561 taacagcgtc tggaacgtaa caagtaattc taatctcgac acgctggcgc tgagccattc
     1621 aactgtcgat tttgccagcc acgggtcaac tgccggcaca tttaccacat taaacgtaga
     1681 gaacctgagc ggtaacagta cctttattat gcgtgctgat gttgttggcg agggtaatgg
     1741 cgttaagccc tgggcctgat gtggtacgtt ccatgtctcc cgtttcatca gcttattgca
     1801 tcgctcccag gaggttgcaa ttaagggaga tatgccccga ccatcgttgt taaaagcgcc
     1861 actcatatcc cgcatccctt atgacaaaag gtgttccggc tcgcaatttt aagtattaat
     1921 acacgtaaaa gtttcaatat ggaacattca ttaaagcata tgtttcacta tgaaacataa
     1981 tttgggctaa ttttcttttc ccttgcctga tgcacaatgg attcatcggc agtcagtggt
     2041 cgccgtgtcg ttgaacatca tccatgccct accgtaattg ctggagcaaa ataatgaaaa
     2101 aattgatcaa tgatgtgcaa gacgtactgg acgaacaact ggcaggactg gcgaaagcgc
     2161 atccatcgct gacactgcat caggatccgg tgtatgtcac ccgagctgat gcccctgttg
     2221 caggaaaagt cgccctgctg tcgggtggcg gcagcggaca cgagccgatg cactgtggtt
     2281 atatcggtca ggggatgctt tcgggggcct gtccgggcga aattttcacc tcaccgacgc
     2341 ccgataaaat ctttgaatgc gccatgcaag ttgatggcgg cgaaggtgta ctgttgatta
     2401 tcaaaaatta caccggcgat attcttaact ttgaaacagc gaccgagtta ctgcacgata
     2461 gcggcgtaaa agtgaccact gtggtcattg atgacgacgt tgcagtaaaa gacagtcttt
     2521 ataccgccgg gcggcgcggc gttgccaaca ccgtattaat tgaaaaactc gtaggcgcag
     2581 cggcggagcg tggcgactca ctggacgcct gtgcggaact ggggcgtaag ttgaataatc
     2641 aaggccactc aataggtatc gctctcggtg cctgtaccgt tcctgccgcg ggcaaacctt
     2701 cttttaccct ggcggataat gagatggagt ttggcgtcgg cattcatggt gagccgggta
     2761 ttgaccgtcg ccccttctct tcccttgatc aaaccgtcga tgaaatgttc gacaccctgc
     2821 tggaaaatgg ctcataccat cgcactttgc gtttctggga ttatcaacaa ggcagttggc
     2881 aggaagaacc acaaaccaaa caaccgctcc agtctggcga cggggtgatt gcgctggtta
     2941 acaatcttgg cgcaactcca ctttctgagc tgtacggcgt ctataaccgc ctgaccacac
     3001 gttgccagca agcgggattg actatcgagc gtaatttaat tggcgcgtac tgcacctcac
     3061 tggatatgac cggtttctca atcactttac tgaaagttga tgacgaaacg ctggcactct
     3121 gggacgcccc ggtccacacc ccggccctta actggggtaa ataaggagaa agcaatgtca
     3181 ctgagcagaa ctcaaattgt taactggctc actcgttgtg gcgatatttt caacaccgag
     3241 agcgagtatc ttaccggact ggatcgcgag attggcgacg ctgaccacgg gctaaatatg
     3301 aaccgaggct ttagcaaagt ggtggaaaaa ctccctgcta ttgcagataa agatatcggt
     3361 ttcattctca agaataccgg tatgacgctg ctttcctgcg tcggtggtgc cagtggtccg
     3421 ctgttcggta ccttctttat ccgcgccgca caggcgaccc aggcacggca aagcctgacg
     3481 ctggaagagc tttatcagat gttccgcgat ggcgcggacg gcgtaatcag tcgcgggaaa
     3541 gccgaacctg gcgataaaac catgtgtgat gtgtgggtgc cggtggtgga atcgttacgt
     3601 cagtccagcg agcaaaatct ctctgttccg gcggcgctcg aagctgccag tagcatcgcc
     3661 gaattcgctg cacaaagtac gattacgatg caagcccgca aaggccgcgc cagttatctc
     3721 ggtgaacgca gtattggtca ccaggatccc ggcgcaacct cggtgatgtt tatgatgcaa
     3781 atgttggcgt tagccgcaaa agagtaagga attggtgatg gtaaacctgg tcatagtttc
     3841 acatagcagc cgactgggag aaggtgtcgg tgaattagcc cgtcagatgt taatgagtga
     3901 taattgtaaa atcgccattg ctgcgggaat tgacgatcca caaaatccca ttggtaccga
     3961 tgccgtcaaa gtgatggagg ccatcgaatc tgttgctgat gccgaccatg tgctggtcat
     4021 gatggatatg ggtagcgcat tattgagtgc tgaaactgcg ctggaattgc tggctcccga
     4081 gatcgccgca aaagtacgtt tgtgtgctgc gccgttggtc gaaggtacac tggcagcaac
     4141 tgtcagcgcg gcctcggggg cggagatcga caaagttatc tttgacgcca tgcatgcgct
     4201 ggaagccaaa cgtgaacaac tgggtttacc gtcctccgac actgaaatct ctgactcatg
     4261 tcctgcgtac gatgaagaag cccgttctct ggcggtggtc attaaaaacc gtaacggcct
     4321 gcacgtccgt ccggcctccc ggctggttta taccttatcg acatttaatg ccgatatgtt
     4381 gctggaaaaa aacggcaaat gcgtcacacc ggagagtatt aaccagattg cgttactaca
     4441 agttcgctat aacgatacgc tgcgcctgat tgcgaaaggg ccagaagctg aagaggcact
     4501 gatcgctttc cgtcagctgg ctgaagataa ctttggtgaa acggaggaag ttgctccacc
     4561 tactctgcgt cccgttccgc ctgtttcggg taaagccttt tattatcaac cagttttatg
     4621 tacggtacag gcaaaatcac ccctgaccgt ggaagaagaa caggaacgat tacgccaggc
     4681 cattgacttc acgttattag atctgatgac gttaacagca aaagcagaag ccagcgggct
     4741 tgacgatatt gccgcaatct tttctggtca ccatacactg ttagatgatc cggaactgct
     4801 ggcggcggca agcgaactcc ttcagcacga acattgcacg gcagaatatg cctggcagca
     4861 agttcttaaa gaacttagcc agcaatacca gcaactggat gatgaatatc tacaagctcg
     4921 ctatattgat gtggacgatc ttctgcatcg caccctggtc cacctgaccc aaacgaaaga
     4981 agaactcccg cagtttaact cgccaactat tctactggcg gagaacattt atccctcccc
     5041 agtactgcaa ctggatccgg cggttgtaaa aggtatctgc cttagcgccg gaagtccggt
     5101 atcccacagc gccctgatcg cccgtgaact ggggattggc tggatttgcc agcagggtga
     5161 gaaactgtat gcgatacaac cagaagaaaa gctaacgctg gacgttaaaa cgcaacgttt
     5221 caaccgtcag ggttaagcca ccgttcggat ggcatcgttc tgatgtcatc cggcaattta
     5281 ctcagcacta atgtttttta actacctgtt atatttttca ccaatttatg atgatttttc
     5341 ttcgctaatc gcgaaccaaa cgacgctggc tggctaataa acagacgtta tttgatcact
     5401 ctatttcgcc gttatttatc cctttaattc ccttttctaa aatgcctgac agttcgcaga
     5461 atgagatttc gatcatgcag ctagtgcgat cctgaactaa ggttttctga tacttgaata
     5521 ccgttttatt cggtttcgcc taaggagaat gattgatgaa atcccccgca ccttctcgcc
     5581 cgcaaaaaat ggcgttaatt ccagcctgca tctttttgtg tttcgctgcg ctatcggtgc
     5641 aggcagaaga aacaccggta acgccacagc cgcctgatat tttattaggg ccgctgttta
     5701 atgatgtgca aaacgccaaa ctttttccgg accaaaaaac ctttgccgat gccgtgccga
     5761 acagcgatcc gctgatgatc ggatgcagca aaaccagagc ggatttgatc tgcgccattt
     5821 cgttaacgtc aatttcaccc tgccgaaaga gggcgagaaa tatgttccgc cagagggaca
     5881 gtcactgcgc gaacatattg acggactttg gccggtatta acgcgttcta ccgaaaacac
     5941 cgaaaaatgg gattctctgt taccgctgcc ggaaccttat gtcgtgccag gcggacgttt
     6001 tcgcgaggta tattactggg acagttactt caccatgtta ggacttgccg aaagcggtca
     6061 ctgggataaa gtcgcggata tggtggccaa ttttgctcat gaaatagaca cttacggtca
     6121 tattcccaac ggcaaccgca gttactattt aagccgctcg cagccgccct tctttgccct
     6181 gatggtagag ttactggcgc agcatgaagg cgatgccgca ttgaagcaat acctgccgca
     6241 aatgcaaaaa gaatatgctt actggatgga cggtgttgaa aacctgcaag ccggacaaca
     6301 ggaaaaacgc gttgtaaaac ttcaggatgg tacccttctc aaccgctact gggacgatcg
     6361 cgatacgcca cgaccagagt catgggtgga agatattgcc accgctaaaa gcaatccgaa
     6421 tcgacctgcc actgaaattt accgcgacct gcgctctgct gctgcgtctg gctgggattt
     6481 cagctcgcgc tggatggaca atccgcagca actaaatacc ttacgcacca ccagcatcgt
     6541 accggtcgat ctgaacagcc tgatgttcaa aatggaaaaa atccttgccc gcgccagcaa
     6601 agctgccgga gataacgcga tggcaaacca gtacgaaaca ctggcaaatg cccgtcaaaa
     6661 agggatcgaa aaatacctgt ggaacgatca acaaggctgg tatgccgatt acgacctgaa
     6721 aagtcataaa gtgcgcaatc agttaaccgc ggccgccctg ttcccactgt acgtcaatgc
     6781 ggcagcgaaa gatcgcgcca acaaaatggc gacggcgacg aaaactcatc tgctgcaacc
     6841 cggcggcctg aacaccacgt cggtgaaaag tgggcaacaa tgggatgcgc caaatggctg
     6901 ggcaccgtta cagtgggtcg cgacagaagg attacaaaac tacgggcaaa aagaggtggc
     6961 gatggacatt agctggcact tcctgaccaa tgttcagcac acctatgacc gggagaaaaa
     7021 gctggtggaa aaatatgatg tcagcaccac cggaacgggg ggcggcggtg gcgaatatcc
     7081 attacaggat ggctttggct ggaccaatgg cgtgacgctg aaaatgctgg atttgatctg
     7141 cccgaaagag caaccgtgtg acaatgttcc ggcgacgcgt ccactcagtg aatcaacaac
     7201 acagccggta aaacaaaaag aggcggaacc tacgccttaa ctttgctgta tatcgagcaa
     7261 tgccattatg tgcataaaat tggcattgct cttttttcgc cacgaaatgc cccccat
//
LOCUS       DAZFDC010000181         7237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFDC010000181 DAZFDC010000000
VERSION     DAZFDC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.181"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            79..1412
                     /gene="adeQ"
                     /locus_tag="ACHJ48_004354"
                     /gene_synonym="yicO"
                     /pseudo
     CDS             79..1412
                     /gene="adeQ"
                     /locus_tag="ACHJ48_004354"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
     gene            1465..1917
                     /gene="yicN"
                     /locus_tag="ACHJ48_004355"
     CDS             1465..1917
                     /gene="yicN"
                     /locus_tag="ACHJ48_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO4709778.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2033..2356)
                     /locus_tag="ACHJ48_004356"
     CDS             complement(2033..2356)
                     /locus_tag="ACHJ48_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4709779.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVNAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2340..2693)
                     /locus_tag="ACHJ48_004357"
     CDS             complement(2340..2693)
                     /locus_tag="ACHJ48_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4709780.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGVSNWFYTFLYAKQDMSNINSQELAGFREIAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2927..4117
                     /gene="nepI"
                     /locus_tag="ACHJ48_004358"
     CDS             2927..4117
                     /gene="nepI"
                     /locus_tag="ACHJ48_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO4709781.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGCFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMTLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4158..4451)
                     /gene="yicS"
                     /locus_tag="ACHJ48_004359"
     CDS             complement(4158..4451)
                     /gene="yicS"
                     /locus_tag="ACHJ48_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO4709782.1"
                     /translation="MKPTMLLMITVFLIFPALSQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4673..5491
                     /gene="nlpA"
                     /locus_tag="ACHJ48_004360"
     CDS             4673..5491
                     /gene="nlpA"
                     /locus_tag="ACHJ48_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO4709783.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNNGELDANVFQHRPFLEQDNQTHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIEQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5495..6418)
                     /gene="yicL"
                     /locus_tag="ACHJ48_004361"
     CDS             complement(5495..6418)
                     /gene="yicL"
                     /locus_tag="ACHJ48_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO4709784.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFTAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYAGQGTNFIVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(6529..6702)
                     /locus_tag="ACHJ48_004362"
                     /pseudo
     CDS             complement(6529..6702)
                     /locus_tag="ACHJ48_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6882..6987
                     /locus_tag="ACHJ48_004363"
                     /pseudo
     CDS             6882..6987
                     /locus_tag="ACHJ48_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
     gene            6998..7063
                     /locus_tag="ACHJ48_004364"
                     /pseudo
     CDS             6998..7063
                     /locus_tag="ACHJ48_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
BASE COUNT         1768 a         1606 c         1811 g         2052 t
ORIGIN      
        1 gtgataatta tcactgaacc gttgttgtca tttgttttac aaaagcaagg gattaaatct
       61 cctccaatgg acaaaaaaat gaataatgac aataccgatt acgtgagtaa tgaatcaggg
      121 acgctttcgc gattatttaa actacctcag catgggacca ccgtccgcac agaattgatt
      181 gcggggatga ccactttttt aaccatggtg tacatcgttt ttgtgaaccc gcaaatcctc
      241 ggcgcggcac aaatggaccc gaaagtggtg tttgttacca cctgtttgat tgccggtatc
      301 ggcagtattg cgatggggat atttgctaac ttacccgtgg cgctggctcc ggcaatgggg
      361 ctgaacgcct tctttgcgtt cgtggtcgtg ggggcgatgg gtatctcctg gcagaccggg
      421 atgggcgcga tattctgggg cgcaattggg ctatttttgc tcacgctatt tcgtatccgg
      481 tactggatga tctccaacat tccattaagt ttacgtattg gtatcaccag cggaatcgga
      541 ttatttatcg ccttaatggg attaaaaaat actggcgtta ttgtcgccaa taaagacact
      601 ctggtgatga ttggcgattt aagttctcac ggcgtgttgt taggtatttt agggtttttt
      661 attataaccg tgttgtcatc acgtcatttt catgccgcgg tgcttgtttc tattgtggtg
      721 acgtcttgct gtggattatt tttcggtgat gttcatttta gcggcgtcta ttccattccg
      781 cctgatatta gcggcgtcat tggtgaagta gatttgagcg gcgcgttaac acttgaactc
      841 gccggtatca ttttctcctt tatgctgatc aacctatttg attcatcagg aacattaatt
      901 ggtgtaactg ataaagctgg cttaatagat agtaacggta aattccccaa tatgaataag
      961 gcgctatatg ttgatagcgt agttcggtgg cgggcgcgtt tatcggcacc tcgtctgtta
     1021 ccgcctatat tgaaagtact tctggtgtgg cagtcggtgg tcgcacgggg ctgactgcgg
     1081 ttgtggtcgg cgttatgttc ctgttggtta tgttcttctc accgctggtg gcgatggttc
     1141 ctccttacgc aaccgccgga gcgttaatct ttgttggcgt gttgatgacc tcaagtctgg
     1201 cgcgcgttaa ctgggatgat tttaccgaat cggtgcctgc gtttgttacc acggtgatga
     1261 tgccctttac tttctcgatc accgaaggga ttgcacttgg ctttatgtcg tactgcatca
     1321 tgaaagtgtg caccgggcgc tggcgcgatc tgaacctgtg tgtggtggtg gtcgcagctc
     1381 tgtttgcact gaagattatt ctggtggatt agtaactggc gagggtttcc agatatcatg
     1441 agttctgatt acgcaggaga actcatgatc tggataatgc tcgccacgct ggcggtagtg
     1501 tttgtggttg gttttcgggt gctgacatcc ggggccagaa aagcgattcg ccgtctcagc
     1561 gatcggctga atatcgatgt cgtacccgtg gagtcgatgg tcgatcaaat gggaaagtca
     1621 gccggtgacg aatttttacg ttatttgcat cgtccggatg agtcgcacct gcaaaacgcc
     1681 gcgcaggtgt tgctcatctg gcaaattgtc attgtcgatg gtagcgaaca gaacctgctg
     1741 caatggcatc ggattttaca aaaagcccgc cttgccgctc ccattactga tgctcaggtc
     1801 aggctggcgc tgggttttct gcgcgaaacc gaacctgaaa tgcaggatat taatgctttt
     1861 cagatgcgct ataacgcgtt ctttcagcct gccgagggcg ttcactggct gcattaaaac
     1921 cgcttcgtct tatcagccta cagaccgcgc ctgacctgta gggtggataa ggcgttcacg
     1981 ccgcatccgg tatcggaact gacagtaggt gaacgcatta tctacgttct atttacagca
     2041 gcacctccaa cccgtgtttt tgcacaatgc tgagcagttt taatgaaatg ccattggggc
     2101 gtttagcgcc actttcccat ttttgtacag ttgaaacgct ggtattgaga tagtgagcga
     2161 agacgggctg actcactttt aatttttccc gcagtgcttt tatctcaaca ggttgtaagt
     2221 cttgcacgct ggtcaggcag gctttgtcaa aagtacgcat cgtttcagca gaaatagcat
     2281 tgacgctgga caatccagcc gctgcgctgt gaatagcctc aaatgcagga cttttaaatt
     2341 tagtttttgc agtcatagca gatctctgtc agttctttag tgttaatcat tgctgttagt
     2401 tgggctttcg tcaggaaggc ataatgcttt gcaatttcac gaaacccagc cagttcctga
     2461 ctattgatgt tcgacatatc ctgttttgca tataaaaacg tgtaaaacca attgcttacc
     2521 cctttcgcca gaataattgc acgctcacga ttttggtgaa ggcgcttttt aaaaacgcca
     2581 ccgccaagtg catcagcttt accttgttct gtttctaaaa tggcccggca caactcttca
     2641 tctgttattg catgagacct ggcttccctt gcaaaccatt tggttttaaa cacgcgtata
     2701 tcgttgaggt cctgttatta aagtatatca ccaagtgcaa tactttatcc cttgttccca
     2761 acacaatcgc gaaaatacat atcaccttgt cctatgattt ttatttcttc gagcagcctc
     2821 gagcttttcc cacaacaccg taaaagttgc aaaaacgtta aatgcgtcac acatttcaat
     2881 gttaaagtgg tcggcttttc ccctgaaaca tgccacgggt aacaccatga gtgaatttat
     2941 tgccgaaaac cgcggcgcgg atgccatcac ccgaccgaac tggtcagccg ttttctcggt
     3001 ggcgttttgc gtcgcctgtc tgattatcgt tgagtttttg cctgtcagtt tgttgacgcc
     3061 aatggcccag gatttaggca tttcggaagg ggttgccggg caatcggtga ccgtgaccgc
     3121 ctttgtggca atgtttgcca gtttgtttat tacccagaca attcaggcca ctgaccgccg
     3181 ctacgttgtt attttgtttg ccgttttgct gacgctctcc tgcttgctgg tttcctttgc
     3241 taactcattc agtttgcttt taatcggtcg tgcctgtctg gggctggcgc tgggcgggtt
     3301 ctgggcgatg tcggcgtcgc tgaccatgcg tctggtgccg ccgcgtacgg tgccgaaggc
     3361 gctgtcggtg atcttcggcg cggtttctat tgcgctggtg attgccgcgc cgttgggctg
     3421 ttttttaggc gagcttatcg gttggcgcaa tgtctttaat gcggcggcgg cgatgggtgt
     3481 gctgtgtatt ttctggatta tcaaatcact gccttcgctg ccaggtgaac cttcgcatca
     3541 gaaacaaaat actttccgct tattacaacg tccgggagtg atggccggga tgatcgccat
     3601 ctttatgtcc tttgccggac agtttgcttt tttcacttat attcgcccag tgtatatgac
     3661 tctggcggga ttcggtgttg atggcttaac cctggtgctg ctgagttttg gtatcgccag
     3721 ctttgtcggg acatcgcttt cgtcgtttat tctcaaacgt tcggtgaaac tggccttagc
     3781 gggcgcaccg tttgtgctcg ccctgagcgc gctggtactg acgctgtggg gaagcgataa
     3841 aattgttgct actggtgtgg cgattatctg ggggctgacc tttgctttaa ttcccgttgg
     3901 ttggtcaacg tggatcactc gctcattggc cgatcaggca gaaaaggctg gatctattca
     3961 ggttgccgtt attcagcttg ccaatacctg cggtgcggca atcggcggtt atgcgttgga
     4021 taacattggt ctgacttcgc cgctgatgtt gtccggcaca ttgatgttgc tgactgcatt
     4081 gttggtgact gcaaaggtga aaatgaagaa atcctgatat caggtaaaga agcgttggtc
     4141 agtggttgtt gccgttctta catatccggg cattctacct tacccaacgc ttcccaataa
     4201 ttgctctgat tattacgttc catcgccacg atggcctcgc gtatatgttg tttattgttt
     4261 tcatctgaca acattaactt ttcccacgct tcatctgata atgacgcctg tggcgtgcaa
     4321 attttacgta aatcttgcaa taccgtggtt ttctcttttg ccgactgtag agaactgaat
     4381 ggcgactcgg cctgagaaag ggctggaaaa atcaggaata cggtaatcat gagtagcatc
     4441 gttggcttca tagcggcaac cttctgcaaa tgtgcatcga atgaagcata agtgtagttt
     4501 gcttttgccg ctctcatcat gctgtacgaa aggtgtttgc gtggtcagta agaagtgcca
     4561 gactttatat tccactttta ttccttttta ttcttatcga tagcgttttg ttttttaaac
     4621 tgcagcgacc ttaccgctat agtccggtaa tcattaataa aaggataaaa aaatgaaact
     4681 gacaacacat catctacggg cgggggccgc attattactg gcaggagttc tgctggcagg
     4741 ttgcgaccag agcagcagcg atgaaaagca tattaaagtt ggcgttatta atggcgcaga
     4801 acaagatgtc gcagaagtcg ctaaaaaggt agcaaaagag aaatatggcc tcgatgttga
     4861 actggtagga tttagcggtt cgttgctacc gaacgatgcg acaaataatg gcgagcttga
     4921 tgcgaacgtc ttccagcatc gaccctttct tgaacaagat aatcagacgc atggttataa
     4981 actggtagcg gtgggaaata cgtttgtctt ccctatggcg ggctattcca aaaaaatcaa
     5041 aaccgtggcg caaataaaag aaggtgcgac agtggcgatc cccaacgacc cgactaacct
     5101 tggtcgcgcg cttttactgc tgcaaaaaga gaagctaatt actctgaaag agggaaaagg
     5161 gttattacct accgcgttgg atataaccga taatccacgc catttgcaga taatggagct
     5221 ggaaggggca cagttgccac gtgtactgga cgacccgaaa gtggatgtag cgattatcag
     5281 caccacttac atcgagcaaa caggactttc tccggttaac gacagcgtat ttattgaaga
     5341 taaaaattcg ccgtatgtga atattctggt ggcacgggaa gataataaga atgcagaaaa
     5401 cgtgaaggaa tttctgcaat cttatcaatc acccgaagtc gctaaagcgg cagaaaccat
     5461 ctttaacggt ggtgcggtgc ctggctggta attctcactc atgccgcgcc ggacgattta
     5521 tttttctggc gcggcggcgg gaatccattg aaatcaaaat caccgatgac agaatcagca
     5581 gcgttcccag ccagtccggc aatgtgaagg tgatccccag caacagcaaa gagagcagcg
     5641 cgctacttaa tggttcagca cagcttaaaa tgctggcttt tggaccgcca attaattgtg
     5701 ctcctttcag gtacagacta aatgtcaggg acgtaccaat gaccaccaaa taaaaaaacg
     5761 ccagaatcaa acttccgtta acgataaagt ttgtcccttg tccggcataa aagggcagca
     5821 ggatcagacc gccaatgagc atactccagc cgacgaccgg taacgtgcca tagcgggcaa
     5881 tcagcgttga gggataggtg gtatagaatg cagcagtaaa tgccgaggca atgccccaga
     5941 ataacgcggc aggagagatc gataatgacg tcggattacc gtgtgtcacc aataaaaaag
     6001 tcccgatcag cgatgtcaaa atagcgcaga aaaccagaat gcccgggcgg gatttacgca
     6061 ccagtgagaa ccaggcgacg ataatagtcg gtgagaggaa ttgcagcacc gttgccgtgg
     6121 ctgcgttcga tttttcgatg gttagcaaaa aagtgagctg tacggttagc gcgccaacca
     6181 cggaaaaaat cagtaggcta atggcatctt tgtgattgtt aataatagaa aagattttat
     6241 caccatgaac aaatgatagc gtcagcagaa tcaaaccggc gaatatcaaa cgcgtcatag
     6301 tcaaaaactg cgacgacatc tggctttgct ccatgatgta ttgcgcgcag acccctgaac
     6361 ttccccacaa cacggcggca atcagaacgt tcagcatccc ctttctggtg gaacccattt
     6421 ttccctctgc atactgtttt gtcgtttatg tatgcaggca tgatagcaaa aatggcgagg
     6481 atggctcagt ttgcgccacg ccgatttttc ttagcccaga ctggtgagct aaatatcttt
     6541 aataaacagc aagcaaatca ttgcaatacc caacatccct atcgccagcc agaacaacgt
     6601 gtggtagctc cagatttcaa ccataatttc gtcaacagag ccggcgataa tctagccgaa
     6661 gcgtgaggta tttgcatata acgttgttgc ggaacctatt cttatttatc ggagtgcctt
     6721 gccagtaatt tactgacgag gatcctcata atagcgttac ccatctgttg cattgagact
     6781 ttacaggccg ttattctaaa tgaccaaaat taaatcagtg cataagggga agtattctgt
     6841 agtgatgtct ccctgagatg caggttccac tggagaaaat tatgagcagt ttgtttcaga
     6901 atgaaataaa ggataaattt aaaactggac ttgcatatgt gcgggggcca gaaaaaagcg
     6961 gatcttccgc tcaaaatgca ggtagcgaag gtggtaaaaa atgcgccggg agaagcagaa
     7021 gattgtcccg cgatgtccgt ttgagaaaca cgagtttgat taaacgcgtg agcatgatga
     7081 tattagtagc tgaccagaga aattatatcc gtcgtaagca ataaaaatca gaagtgatgt
     7141 tatggatatt tttaacctga ataactccgt aagtacgggg ttattcaggt ttttgctagc
     7201 ctgtgctttt ctataccaga tgtggtgtga acactct
//
LOCUS       DAZFDC010000182         7150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFDC010000182 DAZFDC010000000
VERSION     DAZFDC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.182"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            586..1575
                     /gene="araF"
                     /locus_tag="ACHJ48_004365"
     CDS             586..1575
                     /gene="araF"
                     /locus_tag="ACHJ48_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO4709785.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1645..3159
                     /gene="araG"
                     /locus_tag="ACHJ48_004366"
     CDS             1645..3159
                     /gene="araG"
                     /locus_tag="ACHJ48_004366"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO4709786.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3174..4160
                     /gene="araH"
                     /locus_tag="ACHJ48_004367"
     CDS             3174..4160
                     /gene="araH"
                     /locus_tag="ACHJ48_004367"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO4709787.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVA
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4327..5127
                     /gene="otsB"
                     /locus_tag="ACHJ48_004368"
     CDS             4327..5127
                     /gene="otsB"
                     /locus_tag="ACHJ48_004368"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO4709788.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5102..6526
                     /gene="otsA"
                     /locus_tag="ACHJ48_004369"
     CDS             5102..6526
                     /gene="otsA"
                     /locus_tag="ACHJ48_004369"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO4709789.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLV"
     gene            complement(6533..6961)
                     /gene="uspC"
                     /locus_tag="ACHJ48_004370"
     CDS             complement(6533..6961)
                     /gene="uspC"
                     /locus_tag="ACHJ48_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO4709790.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1813 a         1650 c         1842 g         1845 t
ORIGIN      
        1 aatgtaactg caatgaattt taatgatagt gctttttgta tcttgttgat attaatcaat
       61 gaatttgaat tcatgatgaa gtgattattt tataaaacgt tttttcattt ttgcgagatg
      121 gctctcatta tacgtgttct gttaattaat gagcacagtg ataattaatg acgaaatgaa
      181 tgacgtgcat tttccacatc tttgagttgc ggttattaca ccatttcaaa aactcaacgc
      241 caggtaatgc ggcctattga ctggtgaaaa agaagacatc ccgcatgggt accaaagaca
      301 acaaggattt ccaggctaat cttatggatt aatctgctgt gcattcaaca atttgtctga
      361 caaattggct tttccttatg tcttttcccg ctaaatttat gcacgttctc actgtaattc
      421 tgcgatgtga tattgctctc ctatggagaa ttaatttctc gctaaaacta tgtcaacaca
      481 gtcacttatc ttttagttaa aaggtaatgc tttgttttcc gattaattta acgaatgtca
      541 ttcgtttttg ccctgcacaa aacgacacta aagctggaga gaaccatgca caaatttact
      601 aaagccctgg cagccattgg tctggcagcc gttatgtcac aatccgctat ggcggagaac
      661 ctgaagctcg gtttcctggt gaagcaaccg gaagagccgt ggttccagac cgaatggaag
      721 tttgccgata aagccgggaa ggatttaggg tttgaggtta ttaagattgc cgtgccggat
      781 ggcgaaaaaa cattgaacgc gatcgatagc ctggccgcca gtggcgcaaa aggtttcgtt
      841 atttgtactc cggacccgaa actcggctct gccatcgtgg cgaaagcgcg tggctacgat
      901 atgaaagtca ttgccgtgga tgaccagttt gttaacgcca aaggtaagcc aatggatacc
      961 gttcctctgg tgatgatggc ggcgaccaaa attggcgaac gtcagggcca ggaactgtat
     1021 aaagagatgc agaaacgtgg ctgggatgtc aaagaaagcg cggtgatggc gattacctcc
     1081 aacgaactgg atacggctcg tcgccgtact acgggttcta tggatgcgct gaaagctgca
     1141 ggattcccgg aaaaacaaat ttatcaggta cctaccaaat ctaacgacat ccccggggca
     1201 tttgacgctg ccaactcaat gctggttcaa catccggaag ttaaacattg gctgatcgtc
     1261 ggtatgaacg acagcaccgt gctgggcggc gtacgcgcga cggaaggtca gggctttaaa
     1321 gcggccgata tcatcggtat cggtattaac ggtgtggatg cggtgagcga actgtctaag
     1381 gcacaggcaa ccggcttcta cggttccctg ttgccaagcc cggacgtaca tggttataag
     1441 tccagcgaaa tgctttacaa ctgggtagca aaaggtgttg aaccgaccaa attcaccgaa
     1501 gttaccgacg tggtgctgat cacgcgtgac aactttaaag aagaactgga gaaaaaaggt
     1561 ttaggcggta agtaatttgc cggaaaaatt cccctttgca tgatgcagag ggggtgtgaa
     1621 cgaccagtga ttcacggaga cgttatgcaa cagtctaccc cgtatctctc atttcgcggc
     1681 atcggtaaaa cgtttcccgg cgttaaggcg ctgacggata ttagttttga ctgctatgcc
     1741 ggtcaggttc atgcgttgat gggtgaaaat ggcgcaggaa aatcaactct cttaaaaatc
     1801 ctcagcggca actatgcgcc aaccacgggt tctgtagtga ttaatgggca ggaaatgtcc
     1861 ttttccgaca cgaccgcagc acttaacgcg ggtgtggcga ttatttacca ggaactgcat
     1921 ctcgtgccgg aaatgaccgt cgcggaaaac atctatctcg gccagctgcc gcataaaggc
     1981 ggcattgtga atcgctcatt gctgaattat gaggcgggtt tacaacttaa acatcttggt
     2041 atggatattg acccggacac gccgctgaaa tatctctcca ttggtcagtg gcagatggtt
     2101 gaaatcgcca aggcgctggc gcgtaacgct aaaattatcg cctttgatga gccaaccagc
     2161 tccctttctg cccgcgaaat cgacaatctt ttccgcgtta ttcgtgaact gcgaaaagag
     2221 gggcgggtga tcttatacgt ttctcaccgt atggaagaaa tatttgccct cagcgatgcc
     2281 atcactgtct ttaaagatgg acgttatgtc aaaaccttta ccgatatgca gcaggttgac
     2341 cacgacgcgc tggtgcaggc gatggtcggg cgcgacattg gcgatatcta cggctggcaa
     2401 ccgcgtagtt atggcgagga gcgcctgcgt cttgatgctg tgaaagcacc aggcgtgcgt
     2461 acgccaataa gtctggcggt tcgcagtggt gaaattgttg ggctgtttgg tctggtaggg
     2521 gcggggcgta gcgaattaat gaaaggcatg tttggcggga cgcaaatcac cgccggtcag
     2581 gtttatatcg accaacagcc gatcgatatt cgtaaaccga gccacgccat tgccgcaggc
     2641 atgatgctct gcccggaaga tcgcaaagcg gaaggcatta ttcctgtgca ctccgttcgc
     2701 gacaatatca acatcagtgc cagacgtaaa catgtgctcg gcggttgtgt aatcaacaac
     2761 ggttgggaag aaaacaatgc cgatcaccac attcgttcgc tcaacatcaa aacgccgggc
     2821 gctgagcaac tgattatgaa tctctcaggt ggaaatcagc aaaaagccat tctgggccgc
     2881 tggttatcgg aagagatgaa ggtcattttg ctggatgaac ctacgcgcgg cattgatgtt
     2941 ggtgctaagc acgaaatata taacgtgatt tatgcgctgg cggcgcaggg tgtggcggtg
     3001 ctgtttgcct ccagcgactt acctgaagtc ctcggcgttg ccgaccggat tgtggtgatg
     3061 cgcgaaggtg aaatcgccgg tgaattgtta cacgagcagg cagatgagcg tcaggcactg
     3121 agccttgcga tgcctaaagt cagccaggca gttgcctgag taaggagagt atgatgtctt
     3181 ctgtttctac atcgggttct ggcgcaccta agtcgtcatt cagcttcggg cgtatctggg
     3241 atcagtacgg catgctggtg gtgtttgcgg tgctttttat cgcctgtgcc atttttgtcc
     3301 cgaactttgc cacctttatt aatatgaaag ggttgggcct ggcaatttcc atgtcgggga
     3361 tggtggcgtg tggcatgttg ttctgccttg cttccggtga ctttgacctt tctgtcgcct
     3421 ccgtaattgc ctgtgcgggt gtcaccacgg cggtggttat caatctgact gaaagcctgt
     3481 ggattggcgt ggcagcgggg ttgctgcttg gcattctctg tggcctggtc aatggctttg
     3541 ttatcgccaa actgaaaata aatgctctga tcacgacact ggcaacgatg cagattgttc
     3601 gaggtctggc gtacatcatt tccgacggta aagcggtcgg tatcgaagat gaaagcttct
     3661 ttgcccttgg ttacgccaac tggttcggtc tgcctgcgcc aatctggctc accgtcgcgt
     3721 gtctgattat ctttggtttg ctgctgaata aaactacctt tggtcgtaat accctggcga
     3781 ttggcgggaa cgaagaggct gcgcgtctgg cgggtgtacc ggttgctcgc accaaaatta
     3841 ttatctttgt tctctctggc ctggtatctg cgatagccgg aattattctg gcttcacgta
     3901 tgaccagtgg gcagccaatg acgtcgattg gttatgagct tattgttatc tccgcctgcg
     3961 ttttaggtgg cgtttctctg aaaggtggca tcggaaaaat ctcatatgtg gtggcgggta
     4021 tcttaatttt aggcaccgtg gaaaacgcca tgaacctgct taatatttct cctttcgcgc
     4081 agtacgtggt tcgcggctta atcctgctgg cagcggtgat cttcgaccgt tacaagcaaa
     4141 aagcgaaacg cactgtctga tgcttttttc cacaacaatt taacgttttt tcccaccaca
     4201 gccagccgcc acaacggttg gctgttcttc gttgcaaatg gcgacccccg tcacactgtc
     4261 tatacttaca tgtctgtaaa gcgcgttctg cgcaacacaa taagaaaaga gaaggaggag
     4321 aaccgggtga cagaaccgtt aaccgaaacc cctgaactat ccgcgaaata tgcctggttt
     4381 tttgatctcg atggaacgct ggcggaaatc aaaccgcatc ccgatcaggt cgtcgtacct
     4441 gacaatattc tgcaaggact tcagctactg gcaaccgcaa gtgatggtgc attggcattg
     4501 atatcagggc gctcaatggt ggagctggac gcactggcaa aaccttatcg cttcccgtta
     4561 gcgggcgtgc atggggcgga gcgccgtgac atcaatggta aaacgcatat cgttcatctg
     4621 ccggatgcga ttgcacgtga tattaacgtg caactgcata cggtcatcgc ccagtatccc
     4681 ggtgcggagc tggagacgaa agggatggct tttgcgctgc attatcgtca ggctccgcag
     4741 catgaagacg cattaatgac attagcgcaa cgtattactc agatctggcc acaaatggcg
     4801 ttacagcagg gaaagtgtgt tgtcgagatc aaaccgagag gtaccagtaa aggtgaggcg
     4861 attgctgcct ttatgcagga agctcccttt atcgggcgaa cgcccgtatt tctgggcgat
     4921 gatttaaccg atgaatctgg cttcgcagtc gttaaccgac tgggcggaat gtcagtaaaa
     4981 attggcacag gcgcaactca ggcatcatgg cgactggcgg gcgtaccgga tgtctggagc
     5041 tggcttgaaa tgataaccac cgcattacaa caaaaaagag aaaataacag gagtgatgac
     5101 tatgagtcgt ttagtcgtag tatctaaccg gattgcacca ccagatgagc acgccgccag
     5161 tgccggtggc cttgccgttg gcatactggg ggcactgaaa gccgcaggcg gactgtggtt
     5221 tggctggagt ggtgaaacag ggaatgagga tcagccgcta aaaaaggtga aaaagggtaa
     5281 cattacgtgg gcctctttta acctcagcga acaggacctt gacgaatact acaaccaatt
     5341 ctccaatgcc gttctctggc ctgcttttca ttatcgcctc gatctggtgc aatttcagcg
     5401 tcctgcctgg gacggctatc tacgcgtaaa tgcgttgctg gcagataaat tactgccgct
     5461 gttgcaagac gatgacatta tctggatcca cgattatcac ctgttgccat ttgcgcatga
     5521 attacgcaaa cggggagtga ataatcgcat tggtttcttt ctgcatattc ctttcccgac
     5581 accggaaatc ttcaacgcgc tgccgacata tgacaccttg cttgaacagc tttgtgatta
     5641 tgatttgctg ggtttccaga cagaaaacga tcgtctggcg ttcctggatt gcctttctaa
     5701 cctgacccgc gtcacgacac gtagcgcaaa aagccataca gcctggggca aagcgtttcg
     5761 aacagaagtt tacccgattg gcattgagcc aaaagaaata gccaaacagg ctgccgggcc
     5821 actgccgcca aaactggcgc aacttaaagc ggaactgaaa aacgtacaaa atatcttttc
     5881 tgtcgaacgg ctggattatt ccaaaggttt gccagagcgt tttctcgcct atgaagcgtt
     5941 gctggaaaaa tatccgcagc atcatggtaa aattcgttat acccagattg caccaacgtc
     6001 gcgtggtgat gtgcaagcct atcaggatat tcgtcatcag ctcgaaaatg aagccggacg
     6061 aattaatggt aaatacgggc aattaggctg gacgccgctt tattatttga atcagcattt
     6121 tgaccgtaaa ttactgatga aaatattccg ctactctgac gtgggcttag tgacgccact
     6181 gcgtgacggg atgaacctgg tagcaaaaga gtatgttgcc gctcaggacc cagccaaccc
     6241 gggcgttctt gttctttcgc aatttgcggg agcggcaaac gagttaacgt cggcgttaat
     6301 tgttaatccc tacgatcgtg acgaagttgc agctgcgctg gatcgtgcat tgactatgtc
     6361 gctggcggaa cgtatttccc gtcatgcaga aatgctggac gttatcgtga aaaacgatat
     6421 taaccactgg caggagtgct tcattagcga cctaaagcag atagttccgc gaagcgcgga
     6481 aagccagcag cgcgataaag ttgctacctt tcctaagctt gtgtaggagc ggttaatctc
     6541 ccgtaagtgg aactaacagc acatcgacct cacttgaggc aatcactctt ttcgccgagc
     6601 aggatgctcg cgaaaagaaa ctgtgattgt gattaccgca aatcaccaaa tcgaaatgat
     6661 atttgcgaca aacttccaga atatgttcgc ttaactcacc gtaggcaata aacgtcttgt
     6721 caacgggata ccccgcatcc tgaattaact tatcaagaaa gctttgcgtt tcttcctgca
     6781 tcacgctgcg taaatcttca agcatcggcg cagctaattg attgtacatt tccgggtcgg
     6841 aagcgagagt aattaaactg atgtgtccct ttactggcct ggcgatagat accgcttttg
     6901 ccagcagttg ctgactttcc ggtgtaaccg caacagcgac aagaatattg ctatagctca
     6961 taacctgttc cttattctgt gaacttcagg tgacattaaa gctttactcc taatggagaa
     7021 acgacgcaat cccaactcag tcaaacaggt aaatattagt ccgattatta gtaaagacaa
     7081 ttaatcaatc actctcggtg atcatatgat ttcaactgta attataagaa aataatgtac
     7141 tgattccccg
//
LOCUS       DAZFDC010000183         7129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFDC010000183 DAZFDC010000000
VERSION     DAZFDC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.183"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(324..1460)
                     /locus_tag="ACHJ48_004371"
     CDS             complement(324..1460)
                     /locus_tag="ACHJ48_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO4709791.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1463..1825)
                     /locus_tag="ACHJ48_004372"
     CDS             complement(1463..1825)
                     /locus_tag="ACHJ48_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO4709792.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2362..4275
                     /gene="mtlA"
                     /locus_tag="ACHJ48_004373"
     CDS             2362..4275
                     /gene="mtlA"
                     /locus_tag="ACHJ48_004373"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO4709793.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4370..5518
                     /gene="mtlD"
                     /locus_tag="ACHJ48_004374"
     CDS             4370..5518
                     /gene="mtlD"
                     /locus_tag="ACHJ48_004374"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO4709794.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5518..6105
                     /gene="mtlR"
                     /locus_tag="ACHJ48_004375"
     CDS             5518..6105
                     /gene="mtlR"
                     /locus_tag="ACHJ48_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO4709795.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6117..6326)
                     /gene="yibT"
                     /locus_tag="ACHJ48_004376"
     CDS             complement(6117..6326)
                     /gene="yibT"
                     /locus_tag="ACHJ48_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO4709796.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6611..6973
                     /locus_tag="ACHJ48_004377"
     CDS             6611..6973
                     /locus_tag="ACHJ48_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO4709797.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1818 a         1786 c         1803 g         1722 t
ORIGIN      
        1 atctatagat taaatatttt ttaaatgtga ctgtcatcct tcagataata aagccatcat
       61 cgcaatagat ggaaaagcac tcctgctctt ttttgattag agccgtcgca ggaggaacaa
      121 ctcatatcat cattgcgatc tcgacgatgt atagtctcat cctgcggcag aacaagacgg
      181 ataaaaaatc caatgagagc acactcatcc agaacttatt aacataatga atattaaaga
      241 aaaatcatcg cagctaatga agtaatatgc aaaaataatt agtccttaca aggagcaaag
      301 ttccataatc tcccccctcc ccctcaatga tccaaataaa gataatgcat ccagctggtc
      361 attcttagca gcactttccg catcaccgaa acatggctga aatggtcgga gtaaaccgcc
      421 acctgggcgt agatgccgtc gggtaaggca tcgatatcat cgttagggtc cagttcaatg
      481 gttcccagca caccgtccgt gccgggcacg accgttaatg attgcaatac cccctgcgcc
      541 tgataagaac cgcctggcac gacaggtaaa atactagtca gtttgccgtg aaacacctgc
      601 ccaggtagcg cgttaaacac cacttccgca tcatcgccgg gtttcagacg taacagcgag
      661 ttttgccgaa attgggcgac aatttgccgt ttttgctcgg ggatgaagac catcaccgga
      721 cgcagcggca aggcagctgc gtatgtacct gggcggatca gtacctgagt aacgtagcca
      781 ttgctcggcg cgcgaatgac agtctgctca aggttatatt ttgcttcagt aagttgcgct
      841 cttaagctca caatctgcga ttgctcgccg ttaaccatac tgtcgagctg gctctggatc
      901 tgcgcctgct ccgccaccga gcctttcacc agcgcatcct gcgcgaggaa attttgccgc
      961 gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc tttcaagtaa
     1021 cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt gttggcctgc
     1081 gcttcagtga gctgcgcacg cagcgtcttt atattatgcg tcgccgtcat caggtcagcc
     1141 tgaagtctgt caactcgcgc ctggtaacga accgggtcga gcttaaaaag cacctcgccc
     1201 ttttgaataa gctgattatt cttgtcagtg acttcagtaa caattcccgt cacctgtggc
     1261 gtgataggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg gttgtagttc
     1321 atcaataaaa tcaaaccact caccagaaac acgcctccca gcgccgccgt cgccagcgtc
     1381 cactgattta ccggaatgcg gaagatttta aagaccgccc acgccagcgc cacgtaagtt
     1441 aaaacaatca atagatccat aattagatct ccgggaacgt ggaatagtca gtctttttct
     1501 cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga aagtgaggcg atttccgggt
     1561 cagtcgcttt ctcctgcgac gcaacatgtg actgcatacc ccagccacgc tccggttgat
     1621 agagcgtcgc ccagatccac agaaacggcc agataacatg cagagtaaac aggctcaccc
     1681 agcccgccgt atgaatagcg tcggcatggg ggtgattgcg ctttttggca atcagataag
     1741 ggatgtcatg tatcgcgatg attccataaa aaatcaccag aaagacgaag atcagcactc
     1801 ccaacgcgaa atagtttaga aacatatctg cctcggattc acgtttatca gtgttgtttt
     1861 tgggctggca gccagaaggg agtcaggctg atattttgac aataatccgg gttcgcgatt
     1921 ctcgccataa caccaaagaa taatttttag aggtgatgag ttgcttagtt acataacgat
     1981 tgtatgacga aggcataaca tgctgtagat cacatcaggt gaacgccgta agaaaatatc
     2041 ttgtgattca gatcacaaag attcaacaaa ccatcaaaac aaaaatgtga cactactcac
     2101 atttaaatgc catttttagc gaaaatcgcc gccttgttgc ttttttacac aagcgttttg
     2161 tgatgaacgt cacgtcaatt acctctctac cccctatatt tatgtgattg atatcacaca
     2221 aaaggccgtc gactggacag ttaaccgatt cagtgccaga tttcgcagta tctacaaggt
     2281 ccggctacct ctgccgccac attaacaaaa aacctcgggc ttccagcctg cgcgacagca
     2341 aacataagaa ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca aagctttggt
     2401 cgtttcctca gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg gggtatcatc
     2461 accgcgttat ttattccaac agggtggtta ccgaacgaga cgctggcgaa gctggtcggg
     2521 ccgatgatca cttatctcct gccgctgctg atcggttata ccggtggtaa gctggtaggc
     2581 ggcgaacgtg gcggcgtagt cggtgccatc accaccatgg gcgttatcgt cggcgcagac
     2641 atgccgatgt tcctcggttc tatgattgca ggtccgctgg gcggctggtg cattaagcac
     2701 ttcgaccgct gggtagacgg taagatcaaa tccggttttg agatgctggt gaataacttc
     2761 tccgcaggca tcatcgggat gatcctcgct attctggcat tcctcggcat tggcccgatt
     2821 gttgaaaccc tgtccaaaat gctggctgcg ggcgttaact tcatggttgt ccatgacatg
     2881 ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa caacgccatt
     2941 aaccacggta tcttctcgcc gctgggtatt cagcagtccc atgaactggg taaatcaatc
     3001 ttcttcctga ttgaagctaa cccaggtcca ggtatgggcg tgctgctggc gtacatgttc
     3061 tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg caatcatcca cttcctgggt
     3121 ggtatccacg aaatctactt cccgtatgtg ctgatgaatc cgcgtctgat cctcgcagtc
     3181 atcctcggcg gtatgactgg cgtgttcacg ctgactatcc tgggcggtgg tctggtttct
     3241 ccggcatctc cgggttctat ccttgctgta ctggcgatga caccaaaagg tgcttatttc
     3301 gctaacatcg cgggtgtgtg tgcggcgatg gctgtctcct tcgttgtctc tgctattttg
     3361 ctgaaaacca gcaaagtgaa agaagaagat gatattgaag cagcaactcg tcgtatgcag
     3421 gacatgaaag ctgagtctaa aggtgcatct ccgctgtctg cgggcgatgt gactaacgac
     3481 ctgagccacg tacgtaaaat catcgttgcc tgtgacgccg gtatgggttc cagtgcgatg
     3541 ggcgcaggcg tgctgcgtaa gaaaattcag gatgcaggtc tgtcacagat ttcggtcact
     3601 aacagcgcga tcaacaacct gccgccagat gtggacctcg tcatcactca ccgtgacctg
     3661 accgaacgcg ctatgcgcca ggttcctcag gcacagcata tttcgctaac caacttcctc
     3721 gacagcggcc tgtacaccag cctgactgaa cgtctggttg ctgcccagcg acatactgaa
     3781 aacgaagtga aagtgaaaga cagtctgaaa gacagctttg acgattccag tgctaacctg
     3841 ttcaaactgg gcgcggagaa catcttcctc ggtcgcaaag cagcaaccaa agaagaagcg
     3901 attcgttttg ctggcgagca gctggtgaaa ggcggttacg ttgagccgga gtacgttcag
     3961 gcgatgctgg atcgtgaaaa actgaccccg acttatctgg gtgagtctat cgcggtgcca
     4021 cacggtacgg ttgaagcgaa agatcgcgta ctgaaaacgg gcgtggtgtt ctgccagtac
     4081 ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg ctcgtctggt gattggtatt
     4141 gctgcccgta acaacgagca cattcaggtt atcaccagcc tgaccaacgc actggatgat
     4201 gagtccgtca tcgagcgtct ggcacacacc accagcgtgg atgaagtgct ggaactgctg
     4261 gcaggtcgta agtaatccaa tcctaccctc tcctcacgga gagggtttgg gtgagggaaa
     4321 agcctcaccc cagccctctc gggtaaaaac attgatgaag gttaatacta tgaaagcatt
     4381 acattttggc gcaggtaata tcggtcgtgg ctttatcggt aaactgctgg cagacgcggg
     4441 tatccaactg acgtttgccg atgtcaatca ggtggtactt gatgccctga atgcccgtca
     4501 tagctatcag gttcatgtgg tcggtgaaac cgagcaggta gataccgttt ccggcgtcga
     4561 tgctgtcagc agcattggtg atgatgtcgt tgatctgatt gctcaggtcg atttagtcac
     4621 taccgccgtt ggcccggttg tgctggaacg tattgctccg gctatcgcca aagggctggt
     4681 gaaacgtaaa gaacaaggta atgaatcccc gctgaacatc atcgcctgtg aaaacatggt
     4741 acgcggtacc acgcagctga aaggtcatgt gatgaacgcc ctaccagaag acgccaaagc
     4801 gtgggtagaa gaacacgttg gctttgtcga ttccgccgtt gaccgcatcg taccgccttc
     4861 ggcttcggca actaacgatc cgctggaagt gacggtagaa actttcagcg aatggattgt
     4921 cgataaaacg cagttcaaag gcgcactgcc gaacatccca ggcatggagt taaccgacaa
     4981 cctgatggca tttgtcgaac gtaaactctt caccctgaac acgggtcatg ctataaccgc
     5041 gtacctcgga aaactggccg gtcatcagac cattcgtgac gctattctcg acgagaaaat
     5101 ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgcg gtactgatca agcgctacgg
     5161 ctttgacgca gacaaacatg cggcgtacat ccagaaaatc ctcggtcgtt ttgagaaccc
     5221 gtatctgaaa gatgatgtag agcgcgtagg ccgtcagccg ctgcgtaaac tgagtgctgg
     5281 cgaccgtctg atcaagccat tgctcggtac gctggaatac agccttccgc acaagaatct
     5341 gattcagggg attgctggtg caatgcactt ccgcagtgaa gacgatccgc aggctcagga
     5401 actggcagca ctgatcgctg acaaaggtcc gcaggcggcg ctggcacaga tttccgatct
     5461 tgatgccaac agcgaggttg tatccgaggc ggtaaccgct tataaagcaa tgcaataatg
     5521 gtggaccagg cgcaggacac cctgcgcccg aataacagat tgtcagatat gcaggcaaca
     5581 atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc gtctgaatgc tggcaaaacc
     5641 gtgcgaagct ttctgatcac cgccgtcgag cttttgaccg aggcggtaaa tcttctggtg
     5701 cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg ctgtagaacc gttactcgac
     5761 ggcgacggtc cgctgggcga tctttctgtg cgtttaaaac tcatttacgg gttgggcgtc
     5821 attaaccgcc aggaatacga agatgcggag ctgctgatgg cattgcgtga agaactaaat
     5881 cacgacggca acgagtacgc ctttaccgac gacgagatcc ttggaccctt tggggaactg
     5941 cactgcgtgg cggcgttacc accgccgcca cagtttgaac cagcagactc cagtttgtat
     6001 gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa caatggtcct ttcactgact
     6061 gagctgattt ccagaatcag cttaaaaaaa gcctttcaaa agtaagcaac gtctgcttac
     6121 tgcccctcta cctgcttcgg ccgataaagc cgacgataat actccagacg ttgtagatat
     6181 aacggcacgc tttcatcggg tattccggac ggaatcgcgt tacggggagg aagttttttc
     6241 agatactccc ggaacgcctg gcttgatgcc atgaaatcta cggctttatc gataagaagc
     6301 ggaacgtttt cacctaattt gcccatgatg atatctccgt attaccccgc gccgggaatg
     6361 cgcggccgcc aattttagtt taggttcccg gtaaagatga cattaaggaa aacgtgctga
     6421 atcctcaata atcatccgct gctgtttatt atcttttttc tatagatttc ttaattaatc
     6481 aataaattgc gtagttttta agcatgttaa tgattacgtt acagaactat tgcaatgtca
     6541 tcagttgtga acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag
     6601 aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgtctgga
     6661 cgccatccgc caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga
     6721 gctggaaaaa gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aagttcatag
     6781 ccaaaaactg agtaaagaag cacagaagct gatgaagatg ccgtttcagc gcgcaattac
     6841 caaaaaagag caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt
     6901 gcacccaatg accgcgctgg gccgtgaaat gggcctgcag gagatgactg gattctctaa
     6961 gactgcgttt taagcgcctc agacgactgg ttcgatcata tcaacccgca tctggcttgc
     7021 cttagctggt caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc
     7081 cctttacgtc gatttaccgc tggttgatat tctgaaaatg catttttac
//
LOCUS       DAZFDC010000184         7123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFDC010000184 DAZFDC010000000
VERSION     DAZFDC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.184"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(68..259)
                     /locus_tag="ACHJ48_004378"
     CDS             complement(68..259)
                     /locus_tag="ACHJ48_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4709798.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHJ48_004379"
     CDS             complement(256..444)
                     /gene="dicB"
                     /locus_tag="ACHJ48_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4709799.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1018..1203
                     /locus_tag="ACHJ48_004380"
     CDS             1018..1203
                     /locus_tag="ACHJ48_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709800.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(1390..1779)
                     /locus_tag="ACHJ48_004381"
     CDS             complement(1390..1779)
                     /locus_tag="ACHJ48_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709801.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ACHJ48_004382"
     CDS             complement(1791..1919)
                     /gene="ydfB"
                     /locus_tag="ACHJ48_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO4709802.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(1921..2076)
                     /locus_tag="ACHJ48_004383"
     CDS             complement(1921..2076)
                     /locus_tag="ACHJ48_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4709803.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2354..2641)
                     /locus_tag="ACHJ48_004384"
     CDS             complement(2354..2641)
                     /locus_tag="ACHJ48_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4709804.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(2641..2832)
                     /locus_tag="ACHJ48_004385"
     CDS             complement(2641..2832)
                     /locus_tag="ACHJ48_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO4709805.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(2860..3261)
                     /locus_tag="ACHJ48_004386"
     CDS             complement(2860..3261)
                     /locus_tag="ACHJ48_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4709806.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            3370..3642
                     /locus_tag="ACHJ48_004387"
     CDS             3370..3642
                     /locus_tag="ACHJ48_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4709807.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            3626..4051
                     /locus_tag="ACHJ48_004388"
     CDS             3626..4051
                     /locus_tag="ACHJ48_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4709808.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4258..4713)
                     /locus_tag="ACHJ48_004389"
     CDS             complement(4258..4713)
                     /locus_tag="ACHJ48_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709809.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            4792..5883
                     /locus_tag="ACHJ48_004390"
     CDS             4792..5883
                     /locus_tag="ACHJ48_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4709810.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSGAVQQVMTAG
                     SEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPLRAGANPKK
                     SAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPW
                     LLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            5890..6636
                     /locus_tag="ACHJ48_004391"
     CDS             5890..6636
                     /locus_tag="ACHJ48_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4709811.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            6658..>7123
                     /locus_tag="ACHJ48_004392"
     CDS             6658..>7123
                     /locus_tag="ACHJ48_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4709812.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTE"
BASE COUNT         1878 a         1549 c         1835 g         1861 t
ORIGIN      
        1 agggtgaaca aatccctgcc attgctggca tataaaaatg aaaccggata ttaattacgg
       61 tgctgtttta aagtcctgcc ggtatttcgt tattattagt gtgagtagtt ttatctaccg
      121 gataacagtt accgggaatt ttttgttctg ctgctgcagc catgcattct ttcattgaac
      181 cgtataagcc agtcaccagc tcaagagatt cgccggaaac aagataaact gtcagaacga
      241 gtgcaaatgt tgtattcatt gtttatatcc tttttgcagc aggtccagac gagccagcat
      301 tgaaggaatg catacttcat ttaacaggtc ctgctcgagt tttctctgct taatggcgtc
      361 ttcaataaat gttttgtctc cagtgataac gccaatttcg aaacgaagtt cagacgtgct
      421 ggcattacat gataactttt ccattatcgc gtcctcaaca atgaattttg tgatgcggtg
      481 cctggtgcct ccaggtgacg ttaaccagtt aacaattaac gccggataca gagcattctc
      541 gttactcagc aaatgaccgc tttaccgatt taactgttcc gcgtgcgctg agccgcattc
      601 accgcatcac aaaattcact tttaaaaaag ggcggcagag cagttacgga gtaaaactga
      661 taccgccaaa tgtcaccaga atattgataa cagagggcgt tgcagcgggg ttgtcactta
      721 agcgtatggt caacctgaca acccggtgtc ctcaacgggg aaagaataac cccgccatac
      781 ttaccgccgc gccatttcgc ggattgccac aaccggaagc gcacggtcga attaaattta
      841 acgacggctc taagggagaa ggacttcgcc gtgcactttc gtgttatgcc ctgacttttc
      901 agggatatat cctttcagta aactgtcagt gccggattct tatctgtgtc cggcgcacga
      961 ccacacgtag cagcgtgttg gtctccattt ttaattcagc ccttaatgga ggataaaatg
     1021 ccaaacaaac catgccctgc ctgtaacgct ctttctggct ttttagagaa gggcggttat
     1081 tacattttta actgtccaga acatgttgaa tttcatatat caaaacttga cagtatcatt
     1141 acaaagccaa atcagtatca gtctgattta ctgaataaag agttaaacgc cgcccgagat
     1201 taagggcgta gtggcccatt aaaaatattg caaagaccaa ctattgttga actggattaa
     1261 accagtaatt ctctattgct cttattgtta ccatcggatg attgccgggg gttattttaa
     1321 gttcgggagg aaaaggctct ccatcagcag ccatcattga gtatgtgaaa tccgccgacg
     1381 cagtcccttt catgataatt ccgaattttt caagtaatga gagcagttcc ggtaatcctg
     1441 tgtcatctga ataactttcc tcatagaaag aagcgattgc cgcaggagat gacagttctc
     1501 gctctgcaat ttttttgtag ttttccagaa ttcttttttt gaatactttt cctcgacatg
     1561 aaaacccatc accgaggcaa tccgctttgc agtttcttca gtattctggt tgtttgttac
     1621 cgaaacagag tacaatccgg taaacgcatc gcgcacatta cgagccatat tatcaatgta
     1681 tttcttcgtt gcagattcta attcaacttc gttcagacgt tggtgaagtg catgtgctgc
     1741 aatttcctgg atttctttta gtaaatcttt aaattccatc tttaactccg ttagtcgtta
     1801 gattttgccg aactggaaag cgcctgttta aactcactga agctgagagc ttcctcgcct
     1861 tcggcaaggc cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc gaaatccata
     1921 tcactcacct gagtttcttt ccagccagcg acgggcacca ttttcggttt taaacgtttt
     1981 gcttttggta tacgtcatcg cggtgaatgt accgtcctgg ttgggaaaca cgccgtacac
     2041 cagagattcg ttgttgccaa gatcgatagt atccatgttg acctcatttc cccttaacgc
     2101 cgggtagcgg aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc
     2161 atacgcctcg ggctggctac ttaccccctg accactgctt ggtaactcga agtattgccc
     2221 ggcgttctgt ggggcggggt gggtggttgg tgtatgtaat ctacaataaa aaactgtttt
     2281 tgtgtcaaca gtttttaatt gttgttttgg gcaaaaaaat ccctcgaaag agggagtatg
     2341 aaaattgttc aactcagata gagaagggaa attgtcgccg agaatgtgtc acgcttacaa
     2401 tctcaatgct tgaagcagct actctgtaca ggattatgta gttagggtgg gtcacgatct
     2461 ctctcaatcc agaaacccgt tcgcttggtg gatataagta cggatgctca gatagaggta
     2521 ataccgatgt ttcaatgcgt atttttagtc tacgtgctgc cggtgggttc tccttggcga
     2581 tgtaagttat gatctggcgc aaatcatcgc gagcagacgg tagccataaa atgggtaaca
     2641 ttactcgctc ttgttagtta cagcaatttg agcaataaga ttttccattt cagccattac
     2701 ctcgtcatgt ggaattgcgg ggcgagtgtc tgcaaggctt gacgttactt tagtgcgcaa
     2761 ccattcgttg taactgtttt cttgttcggt agtttcgaat tctgaaacta tcggagaaag
     2821 ggctgtaccc atggcataac tcctcttctt gtactgtggt cacgcccggc ggcttttttg
     2881 tgccgccagc cacctagcaa tggtttcttc cattgatttt ttcttgtctt tgatttcttg
     2941 aagcattttt tcttggtctt cctcaggaaa cgcactaaaa gcttggagca gttcgcgttg
     3001 gcgaggacca attttcatcg tgtcaggggt gaaaatttgc tcacactctt caggaggcaa
     3061 taaaaaccaa tgcaatggat gccctgaaac ctcaaccagt ttatccaaac ttgaggcttt
     3121 aggtgtagcc ttaccgctga cccattgttg aacagtttgt tgtgtcacac caattctacg
     3181 ggcaagctca gcctggctcc atccagtttc ctgaagaagc ttgctgattc tgtacataga
     3241 tacttctagg gcgctcatca ttattcaatt ttacaggtaa atactgttaa aagcatcaca
     3301 ataaaaaact gttgattgca tacagttttt tattgtagtc tttgcttata gtttttagag
     3361 gagggcaaaa tgctagatag cactcgcgaa aaaattaggc agaaatacac tcaggctgaa
     3421 ataggtcgtt atatgggggt cgctcaacag actgtttggc aatggtttag ctttggcgtt
     3481 cccccaaagc aggtaattcc gttatgccaa ctaatgaagt gggaagttac cccgcatgaa
     3541 atccgcccag atatttatcc taacccaacc gacggtttac ctgttggatg taaggttaac
     3601 acatcaaatg cgccggagtt gattcatgaa aatcaagcat gaacacatcc gcatggcgat
     3661 gaatgcctgg gcgcatccgg acggcgaaaa agtaccggct gcgaaaatta ccaaagcgta
     3721 tttcgagctg ggaatgacgt tcccggaact gtatgacgac agccatccgg aagccatggc
     3781 tcgcaatact cagaaaattt tccgctgggt ggagaaagac actcctgatg cggttaaaaa
     3841 aattcaggcg ttgttaccag cgatcgaaaa agcgatgccg cctccgctgg tggcccgaat
     3901 gcgcagccac agttccgctt attttcggga gttggtagag acgaaggaac ggctggtgaa
     3961 agatattgat gatttcgttg catcagcgat cgttctgttc gatcagatga atcgtggtgg
     4021 cccggcagga aacactctgg ctgtgcatta attgggtaat aaatatgagt aatgacaaaa
     4081 aattgacact gagcgtttac gaaaacagtc cgcacatctg gcgtggcggt ttatctgatg
     4141 tggagctggc agagtggttg atacataaag ctaatgcgct gctctggcgt ttgtcagcca
     4201 gagaacagcg caaggaaacc agaataaagc tggctgatgc agaagcgtgt gccgggctta
     4261 ttgaggatta tacaaatctt ggtatttctt cagcagagag tgatcccatt cagcctctga
     4321 gcagggagtc aatccagcac gctggttgta tggcacatct tgtaactgct cgtcaacatg
     4381 aggtgggtat tggatcactt ccggcgggat attcgctgat tccagagctg gttgaagcaa
     4441 gaaaatcagt tcagaaaaag agagatgacg cacttcaatt attgagagag cactatggcg
     4501 cgataccaga atgcgaacag cgtcgatacc ctgaaggtta tgaatggatg cagtctcttt
     4561 ttgaagttcg ctaatcaata tgtcgagacg aaggtatgtt tcggcgcgca gccaggctct
     4621 gtaatccggg agcatttcgg ggctgttaca ccagcggttt gttgctgcaa catttaatac
     4681 atgagcctga taaaggcttt tcaaaaaata catgtcgaac ctcctctggt tctgtcgatt
     4741 gggaaccaca gattatatcc ggaggaaggt tcggcaccag atgaggtagc catgcgtgat
     4801 tacgcaaaag tttctccgcg attctggctg ggagaaacgg ggagagaact tagaaaggcg
     4861 ggtgcagaag cgcaagttgt tgctttttac ctgatgacat cccctcacgc aaatatgctg
     4921 ggtttgtatt acctgccagt tttatacctt gctcatgaaa ccgggcttgg tctggaaggg
     4981 gcttcaaagg ggcttaaaag ggctgttgaa gctggttttt gtagctatga ccatgatgca
     5041 gagatggtct gggtccatga aatggcagcc tggcaggttg gggaaacgtt gaagcctggc
     5101 gataaccgtt gtgcaggtgt caggaatgag tatgcatcat tacctgaaaa cgcttttctg
     5161 tcagtgtttt acgacagata taaaacggat ttccatctgg atgtgaggcg gaataatagc
     5221 cgaaattcgg taaggggctt cgaaggggct tttaaggggc ttcgaagcca agaacaggaa
     5281 caggagcagg agaaagaaca ggaacaggac aaaaacacta tggttcatgg caaaaaaaac
     5341 accacgaacc aggcagggga tgttcagacc gtcaatcctg gtcagccagc aggcacgaca
     5401 ccggaagccg attcgggcgc tgtgcagcag gtgatgaccg cagggtcgga gcaatcacac
     5461 caactgcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg
     5521 gaaagcaccg ggcagtctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
     5581 cgggaatacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag
     5641 gccagattgc gcgagggggt gccaccagag accatgctgg atggtgtgag gcgttacgcg
     5701 agatacctgg cggcgaccgg gaaagcggga acggaatttg ttcagcgagc gacgacgttt
     5761 tttgggccgg accggaattt tgaaaacccc tggttgctcc cggtaagcgg cacgaacaac
     5821 cagcgttgtg tgaatcatat ttctgaaccg gataccgaaa ttccgccggg attcaggggg
     5881 tgatgtggca tgaaaaacat tgcggcagcc ggggttcttg aacgtattcg cagacttgca
     5941 ccacaggcgt cggttccacc gtaccggacg gtggaggagt ggcgggaatg gcaacttgct
     6001 gaaggacgaa aacgcagcga ggagattaac cgccagaatc accagttgcg ggtggaaaaa
     6061 atcctgaatc gttcgggcat ccagcctctg cacagcaaat gctcgtttgc gaattatcag
     6121 gtgcagaacg acgggcaaaa atacgcgctg agccaggcca aatccatagc tgacgaactg
     6181 atgaccgggt gcacgaattt tgtgttcagc ggtaaaaccg gcaccgggaa aaatcacctt
     6241 gcagcggcga tgggtaaccg gctgatggcg aaggggcgca gcgtgattat cgtcaccgtg
     6301 tctgatgtca tgagcgtgtt gcatgacagc tacgacaacg gcaaatccgg ggaaaaattt
     6361 ttacaggagc tttgcagtgt tgatttgctg gtcctggatg aaataggcgt tcagcgggag
     6421 acgaaaaacg agcaggtggt attacaccag ataattgatc gccggacagc atcactgtgc
     6481 agtgtcggga tgttaacaaa cctgaatcat gccgcaatga gtacacttct tggtgagagg
     6541 attatggacc gcatgaccat gaacggtggt cgatgggtga cgtttaactg ggatagctgg
     6601 cgtccaaatg tcagcaatat gagggttgtg aagtaatttt gtccggagga aattttaatg
     6661 gaaaccgtat ctgacgcact gaaagcactg aaaaaagcct cttcacatgt ggtggcagct
     6721 cgccttggaa tcagtcgtga agaggctgtc aacgagctgt gggaactcaa aataaatggc
     6781 gtcgttgata aaactggtca cacctggttt ctggctggcg aaggtgaatc ccgggtaacc
     6841 gaagagcggc cagtaaaatc tgaagcacag gatatgctga ccggggaggt cgaacaaaaa
     6901 gttaccgcag acatgatgat tgagtttatc ggtcaggatg gggctaaaac gtgtgaggaa
     6961 ctggcgggta agttcggtgt cagtactcgc aaggttgctt ccacgctggc ggtggtaacc
     7021 gcaacggggc ggctggcacg cgttaatcag aacggtaaat ttcgttactg catgccgggc
     7081 gataatttac cagcagagcc gaaagccgcg ctggtaacgg aaa
//
LOCUS       DAZFDC010000185         7056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFDC010000185 DAZFDC010000000
VERSION     DAZFDC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.185"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            125..328
                     /locus_tag="ACHJ48_004393"
     CDS             125..328
                     /locus_tag="ACHJ48_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO4709813.1"
                     /translation="MSSIFLTENELQTLTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCIKTLPPREAEPDFGAI"
     gene            328..1375
                     /locus_tag="ACHJ48_004394"
                     /pseudo
     CDS             328..1375
                     /locus_tag="ACHJ48_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1403..1492)
                     /locus_tag="ACHJ48_004395"
     tRNA            complement(1403..1492)
                     /locus_tag="ACHJ48_004395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1456..1458),aa:Ser,seq:cga)
     gene            1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHJ48_004396"
     CDS             1586..2383
                     /gene="mtfA"
                     /locus_tag="ACHJ48_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO4709814.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2484..2559
                     /locus_tag="ACHJ48_004397"
     tRNA            2484..2559
                     /locus_tag="ACHJ48_004397"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2517..2519,aa:Asn,seq:gtt)
     gene            2873..>7056
                     /locus_tag="ACHJ48_004398"
     CDS             2873..>7056
                     /locus_tag="ACHJ48_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO4709815.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVDARRSLAGSRFDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSSQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNRDTKIVNIAPDASNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTFKGTVLGVHT
                     AEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVA
                     GITVNFTMPQDVAANFT"
BASE COUNT         2004 a         1673 c         1725 g         1654 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgctcccgag tcaggctgat
       61 ttttaatgtt caacccggat cgccgctaac ggcatatagt tagcggcggt tgtgagatat
      121 agctatgagc agcatatttt taaccgaaaa tgaattgcaa acgttaacgg gctgcaaata
      181 tgcaagccac cagcgcaact ggttaataaa aaatgggctt ccgttctata ccaatcgtag
      241 tggaaaaccg atcgtcagtc gggaactgtt tacctgtata aaaactttac caccacgtga
      301 ggctgaacct gatttcggtg cgatctaatg ggaagacgaa ggaaaaatcc cgaacacgaa
      361 aaattaccgc caaaggtata ccccaataaa tatagtgtat ggaaaccgac atccagagaa
      421 tctgtaacct taactgcaat cgaggatggt ttagccgcat tatggaaaaa gtatgaagaa
      481 acggttaacc atcgcgatcg cgcaatgaca tttgggcgtt tgtgggaaaa atttctcgcc
      541 agcgcctatt acagcgagct tagtcctaga actcaaaaag attatctgca acatcaaaaa
      601 aagctgctgg ccgtattcgg taaggtgctg gccgattctg taaaaccaga gcacatcaga
      661 cgatacatgg acaaaagagg cgagcagagt aaaacgcagg caaaccatga aaaaagcagt
      721 atgtcgcgcg tttatagttg ggggtatgag cgaggatacg tgaaggctaa cccatgtgca
      781 ggtgtaagta aattcaaggc caaaaaccgc gaacgatatg taaccgacaa agaataccag
      841 gcagtattaa gcgttgcacc tcttcctgtt tttatcgcaa tggaaattgc ctatctgtgt
      901 gcagcgaggg tttccgatgt gttatcgctg aaatgggagc agattggaaa cgacgggatc
      961 tttatccagc aagggaaaac agggaaaaaa cagataaaag catggagtcc acgattacag
     1021 gcggcgatcg aaaaagcaaa acagttacca acatccgcct atgtaatcag caatcaatac
     1081 ggcaaccgat atatgtacaa aggctttaac gaaatgtggg tagaagcaag aaatcacgca
     1141 ggcaaaattt caggtatttt aaccgacttc acctttcatg atctgaaggc gaaaggaatt
     1201 tcagactatg aaggaagcag ccgggataag caacttttct ctggtcacaa aaccgaaggg
     1261 caagtgctaa tctatgacag gaaggttaaa gtttcaccaa cacttgatgt cccgttacct
     1321 gaaaatattc caagaaaata ttccaagtaa ttccaagtgt gatttttgtc actgacttaa
     1381 tgatgtgtaa gtgattgaat tttggcggag agagggggat ttgaaccccc ggtggagttg
     1441 cccccactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     1501 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc
     1561 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     1621 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgtgtctgac
     1681 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     1741 tgttccttta cagggctttg aactggattc attaagaagc tgccggatag cacttctatt
     1801 ttgcctaccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     1861 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     1921 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     1981 ggatatacaa gattcttttg atgcttctgg ttttaacctg attattcatg aagtcgctca
     2041 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga
     2101 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     2161 attggttggc gagaatgcgg cgagcattga tgcttatgct gccagtgatc ctgctgaatg
     2221 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     2281 ttcattgtgg caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca
     2341 cgctaatgat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     2401 taattaacca attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt
     2461 aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     2521 atccgtatgt cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt
     2581 tagcgcaatg tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac
     2641 atcttaagcg ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt
     2701 ttcccctcga ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacatcca
     2761 aaactgccta acgaaaagtt attaattttc aatcatattg ctatcaggat ttacattttt
     2821 tcgctgtgct agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac
     2881 gaagaagaga agtggagaag aaataaatga ccgacaaatc ttatgcggga tgggaattaa
     2941 actacgccgc ttaactgcgg gtatctgcct gataactcaa cttgtgttcc ctatggctgc
     3001 ggcagcacaa ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat
     3061 tgcaaatgcc aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc
     3121 cgaacgtttc ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc
     3181 tcgaggtttt gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga
     3241 aaataattta accccgccac cgggtaatag cagtggcaac cttgagcaac agatagccag
     3301 tacttcacag caaatcgggt ctctgctcgc cgaggatatg aacagcgagc aagcggcaaa
     3361 tatggcgcgt ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg
     3421 cttcggtacc gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca
     3481 gttcgatttt ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac
     3541 tctccatcgt actgacgagc gtacgcagat taacaacggc ttgggttggc gtcatttcac
     3601 tcccacatgg atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc
     3661 ccgcgccggc attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta
     3721 tttgcgactg accaactggc gcagcgcacc tgaactggac aacgattatg aagcacgccc
     3781 ggccaatggc tgggatgtac gcgcagaagg ctggctaccc gcctggccgc accttggcgg
     3841 taaactggtc tatgaacagt attatggcga tgaagtggcc ctgttcgata aagatgatcg
     3901 gcaaagtaat cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac
     3961 cttcagcgcg gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga
     4021 ttttacctgg caacctggaa gcgcgatgca gaaacagctt gacccgaatg aagtcgatgc
     4081 acggcgtagc cttgcaggca gccgttttga tctggtggat cgcaacaaca acatcgttct
     4141 ggaatatcgc aaaaaagaac tggttcgcct gaccctgaca gaccccgtga cagggaagtc
     4201 aggagaagtg aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa
     4261 cgtcgaagcc accgctctgg aagctgccgg tggtaaagtg gttacaacgg gtaaagatat
     4321 tctggttacc ctgccggcgt accggttcac cagtacgcca gaaaccgata acacctggcc
     4381 gattgaagtc accgctgaag atgtcaaagg caatttttcg aatcgtgaac agagcatggt
     4441 agtcgttcag gctcctacgc taagccagaa agattcctcg gtatcgttaa gtagccagac
     4501 gttgagcgcg gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg
     4561 taatcctgtt atcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct
     4621 ttctgactgg aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat
     4681 gtctggcacg ctgacgctga tgccacagct gaacggtgtg gatgcggcta aagcccccgc
     4741 cgtggtgaat atcatttctg tttcgtcatc ccggactcac tcgtcaatta agattgataa
     4801 ggaccgttat ctctccggga atcctatcga ggtgacggta gaactgagag atgaaaatga
     4861 caaacctgtt aaggagcaaa aacagcaact gaataccgca gtcagcatcg acaacgtgaa
     4921 acctggtgtc actacagact ggaaagaaac cgcagatggc gtctataagg caacctatac
     4981 cgcctatacc aaaggcagtg ggcttactgc gaagctgtta atgcaaaact ggaatgaaga
     5041 tttgcatacc gctggattta tcatcgacgc caacccgcag tcagcgaaaa ttgcgacatt
     5101 atctgccagc aataatggtg tgctcgccaa tgagaatgca gcaaacaccg tctcggtcaa
     5161 tgtcgctgat gaaggaagca acccaatcaa tgatcatacc gtcacgtttg cggtattaag
     5221 cggatcggca acttccttta acaatcaaaa caccgcaaaa acggatgtta atggtctggc
     5281 gacttttgat ctgaaaagta gtaagcagga agacaacacg gttgaagtca cccttgaaaa
     5341 tggcgtgaaa caaacgttaa tcgtcagttt tgtcggcgac tcgagtaccg cgcaggttga
     5401 tctgcagaag tcgaaaaatg aagtggtcgc tgacggcaat gacagtgcca caatgaccgc
     5461 gacagttcgg gatgcaaaag gcaacctgct caatgacgtc aaggtcacct tcaatgtcaa
     5521 ttcagcagca gcgaaactga gccaaaccga agtgaatagc cacgacggga tcgccacagc
     5581 tacgctgacc agtttgaaaa atggtgatta tacggttacg gcctctgtga gctctggttc
     5641 tcaggctaat caacaggtga tttttatcgg tgatcaaagt actgctgccc tgaccctcag
     5701 tgtgccttca ggtgatatca ccgtcaccaa cacagctccg ctacatatga ctgcaacctt
     5761 gcaggataaa aatggcaatc cactaaaaga taaagaaatc accttctctg tgccaaacga
     5821 cgtcgcaagt cggttctcga ttagcaacag cggaaaaggc atgacggata gcaacgggac
     5881 tgcaatcgcc tccctgaccg gcacgttagc gggcacgcat atgatcacgg ctcgtctggc
     5941 taacagcaat gtcagcgata cacagccaat gacgtttgtg gcggataaag acagagcggt
     6001 tgtcgttctg caaacatcga aagcggaaat cattgggaat ggcgtggatg agacgactct
     6061 gacagcaaca gttaaagatc cttttgataa cgtggttaaa aatctttcag tagtcttccg
     6121 cacctccccc gcagacacgc aactgagtct gaacgcgcgt aatactaatg agaacggtat
     6181 tgccgaagtt acccttaagg gcacggtttt gggtgttcat acagccgaag ccatactgct
     6241 taacggcaac agagatacga aaatcgtcaa tattgcgccc gatgccagca acgcgcaggt
     6301 caccctgaac atccctgcac aacaggtggt gacgaataac agtgacagcg tgcagctgac
     6361 ggcgacggtg aaagacccgt cgaatcatcc ggtggcggga ataacggtga acttcaccat
     6421 gccacaggac gtggcggcaa actttaccct tgaaaataac ggtattgcca tcactcaggc
     6481 caatggcgaa gcgcatgtca ccctcaaagg caaaaaagcg ggcacgcata ctgtgaccgc
     6541 cacgctgggt aacaataatg ccagcgatgc gcaaccagtc accttcgtgg cggataagga
     6601 cagcgcggtt gtcgttctgc aaacatcgaa agcggaaatc attgggaatg gcgtggatga
     6661 gacgactctg acggcaacag tgaaagatcc ttttgataac gcagtaaaag atctacaggt
     6721 caccttcagt accaaccccg cagatactca acttagtcag agcaaaagca atactaacga
     6781 cagtggtgtg gccgaagtta cctttaaggg cacggttttg ggtgttcata cagccgaagc
     6841 cacactgcct aacggcaaca acgatacgaa gatagtcaat attgcgcccg atgccagcaa
     6901 cgcgcaggtt acgctgaaca tccctgctca acaggtggtg acgaataaca gcgacagcgt
     6961 gcagctgacg gcgacggtga aagatccgtc gaatcatccg gtggcgggaa taacggtgaa
     7021 cttcaccatg ccacaggacg tggcggcaaa ctttac
//
LOCUS       DAZFDC010000186         7034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFDC010000186 DAZFDC010000000
VERSION     DAZFDC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.186"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..480)
                     /locus_tag="ACHJ48_004399"
                     /pseudo
     CDS             complement(1..480)
                     /locus_tag="ACHJ48_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(714..1454)
                     /locus_tag="ACHJ48_004400"
     CDS             complement(714..1454)
                     /locus_tag="ACHJ48_004400"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO4709816.1"
                     /translation="MAERLREIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRR
                     AGSPPGPDAHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADA
                     RRIVSHYEQRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISE
                     ETQNDSCEKILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPG
                     WVVMWDGWFRHQDMAEGYLVMKSLDQEI"
     gene            1374..1847
                     /locus_tag="ACHJ48_004401"
     CDS             1374..1847
                     /locus_tag="ACHJ48_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709817.1"
                     /translation="MQVMPDIRFPVTDSDYLFLYLPQPFGHALRSLLPAVTFFFFTGT
                     FFSLVLNTVGVRGTTPALLFDKIDWQGAVGGVHQDRVSQQPVRLRVSGGIPQVIKCRT
                     VTEVNGGGIFQREEQRMCLTCPCSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            complement(1779..1982)
                     /locus_tag="ACHJ48_004402"
     CDS             complement(1779..1982)
                     /locus_tag="ACHJ48_004402"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFO4709818.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            2160..2333
                     /locus_tag="ACHJ48_004403"
     CDS             2160..2333
                     /locus_tag="ACHJ48_004403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709819.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            2894..3454
                     /locus_tag="ACHJ48_004404"
     CDS             2894..3454
                     /locus_tag="ACHJ48_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4709820.1"
                     /translation="MISVMILYVMSGSRLADNHTLSAGYAQSKVQDFKNIKGVNLQYR
                     YEWDSPVSVVGSFSYMKGDWADSHRDEADDFYRHQADIKYYSFLAGPAYRLNDYISFY
                     GLVGISHTKAKGDYEWRNSVGADESDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNV
                     GYEGTKADIYGKHSVNGFTVGVGYRF"
     gene            3590..4381
                     /locus_tag="ACHJ48_004405"
                     /pseudo
     CDS             3590..4381
                     /locus_tag="ACHJ48_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(4386..5756)
                     /locus_tag="ACHJ48_004406"
                     /pseudo
     CDS             complement(4386..5756)
                     /locus_tag="ACHJ48_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5776..6123)
                     /gene="tnpB"
                     /locus_tag="ACHJ48_004407"
     CDS             complement(5776..6123)
                     /gene="tnpB"
                     /locus_tag="ACHJ48_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4709821.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(6120..6794)
                     /gene="tnpA"
                     /locus_tag="ACHJ48_004408"
     CDS             complement(6120..6794)
                     /gene="tnpA"
                     /locus_tag="ACHJ48_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO4709822.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRTSYSRE
                     FKIRLVKQALQPGAVVARIAREHGINDNLLLKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT         1670 a         1647 c         1829 g         1888 t
ORIGIN      
        1 cgatttatga agaattttac tgttgtacag taccagatac tcagccagtt tcagattgaa
       61 catattcagc tattcaaaga gcaattcttc attaaactca ataaattgtt ccctcagtgt
      121 tcggttaaaa tgctcactta tggcatttat tttcggcgta tagagataag tccatacgaa
      181 cccgacggtg gcttcctgta gcgttttgtc gaattttccc gagaactctt tgcccatcat
      241 cagtgatgat ctggttgata ccaacaagga acaactgggc tacacagcag aagaaatgcc
      301 tgatgatatc acttttaagc gatagtacgg ccagagagta gttgctgtgt tcgtcgagca
      361 tggtaatgaa ataacggtac agctcgccat tctgagctca atagtatcca tgccactgag
      421 cttacaggtc tttatcgggc gatagtgctt tattcttcgg ggctttgtgg atgatttttc
      481 gaaatgttca tgatagttag gtaaagctac cgcatatcat ttgcaaaatg acagatagat
      541 atacttcaat ttgaatctta acaattagta tgggaatgct gtcaatcctc tgtatgcgtc
      601 caccacatat tgaaattttt gttctctaat atattccacg ggcgtcaaga gtgtgaggcc
      661 tgtgctgtta tacagcacca gacgttgtcc cccatctttg acctgtctct tgatcagatc
      721 tcctgatcaa gagacttcat caccaggtaa ccctcagcca tatcctgatg tctgaaccag
      781 ccatcccaca taactaccca accggggctg cttgtgcgtt tgctgtcatg ccatcgcccc
      841 agtttagcca gtttcaaaca ggcccatttc agggtcggtg tttgcgccgg aagctgcttt
      901 ccttccagct taacccacag tagtttccac tctgtcggcg tcagtatttt ctcacagctg
      961 tcattttgtg tttcttcact gatacctccc tgccgcaggc ccagcacccg caccgcgata
     1021 accaccatgc gctcaaggtt atcccgggtc tgcattcgca gcgattccac acatgtacca
     1081 ccacttttcc acgctttgtg gtattcctct atcagccagc gttgctcgta atggctgacg
     1141 atacgtcgcg catcggcggc actcgccact ttttctgatg tcagcggata ccagcaggct
     1201 ccgtcctctc ctgctccccg caacagacat acgtgagcga gagcgcctgg ccgctgttgt
     1261 cgggattttt tatgctgact tcgctgtaac tgatgagcat ccgggcctgg cgggctgccc
     1321 gcccgccttt ttgcatcaca ttcagcgtgt ggcttcccgc ggctgacttt ataatgcagg
     1381 taatgccaga tatccgcttc ccggtcacag acagtgatta cctgtttctg tatctccctc
     1441 agccgttcgg ccatgcgctc cgaagcctgc tgccagcggt aactttcttt ttcttcacag
     1501 ggacgttctt ttcgctggtg cttaacaccg taggtgtccg tggcacgact ccagcactgc
     1561 tgttcgataa gatcgactgg cagggcgctg tcgggggcgt acatcaggac agagtgagcc
     1621 agcagcccgt gcgtcttcgg gttagtggtg gtattcccca ggtcatcaaa tgccgtactg
     1681 tgaccgaagt taatggtggt ggtatcttcc agcgcgagga gcagcggatg tgcctcacat
     1741 gcccttgcag tggcagtaaa tccggcttcg gcaatggctt acggggacac cgacgggtta
     1801 cgtatcaggc ggtacgcacc ttcaacctga gcagtgaagt gggatgattt cacaatagaa
     1861 agacctgcat gctgagcgag agaagaggtc agtgacacaa ggcgtcgtgt acgacgcgga
     1921 tcaccgagac gggcatgtcc agactgctcg ttagccagtg aataacaatc agaaagtacc
     1981 ataacagacc cgaagaaaat gaaatataag agaagatcaa cgggtgaaga aaaagttcaa
     2041 aaaatcgctg ccggggagga aggaaactac cggatggaaa gagtccccct aaagcagact
     2101 gacagacatc acaaatcccc gggtggggat ttgtgtataa gagacaggtt acaggtgtgg
     2161 tgaataagac tgcaaatgtg gcagctcatg ccgtggtgaa tgctgcactg gcggtggtac
     2221 agggaaataa cgcccgtgcc agtgccgcag gtgctgccac tggagacgtg gtggggatga
     2281 tagccacaga gatgtgcgga atatcccctg gcactgttac atgtaaaaga taaataacga
     2341 agctacatcc ttctctttat gaacggcttt atattagttt tgctgacaag attgagctaa
     2401 atccggccaa tgaagaagag caggttattc tttaaaggtt tgataacatg taacacattt
     2461 tgaatattca gtcagtaagc cttaaaaatt tacctggtta tgggctgact gatataacaa
     2521 agtaaatatt cgatggggaa tgccggatgc aatgaacaat gcattaacgt atcgaaataa
     2581 tgatgcagga gcaaactcag tctggtgagt atacattatg ccattgatgc agagtttaaa
     2641 ggaacagggc tggttcgtta tggaacaaca tttgtgcctg aggaaagggg ggggagtatc
     2701 tgaggcaaca gaagtttgtg gggagttcgg tttattacca tgcttggttt tttgtcagtg
     2761 tgttcaaatc gtcaggtgat atatttatag ttttttgatg atgatttcaa ataatgtttt
     2821 tgagatgtgt ggtccttttt gattttcaga aatcatttat taatgaagga gggttaaatg
     2881 aaaaaaactc gttataattt cagtaatgat attatatgtt atgagtggtt caagactggc
     2941 tgataatcat acgctatcgg ctggttatgc tcagagtaag gtacaggact ttaagaatat
     3001 taaaggagtg aatctgcaat atcgatatga gtgggattct ccggtcagtg tcgtcgggag
     3061 tttttcgtat atgaaaggtg attgggcgga cagtcatcgc gatgaagctg acgattttta
     3121 cagacatcag gctgatatta aatattattc atttctcgca ggcccagctt atcgtctgaa
     3181 tgactatatc agtttttatg gtttggtggg gatatctcat actaaagcga agggagatta
     3241 tgagtggaga aacagtgtgg gtgctgatga gtctgatgga tatttaagtg agtctgtatc
     3301 aaaaaaatcc actgattttg cttatgcagc tggtgttatt attaatccgt ggggcaatat
     3361 gtctgtaaat gttgggtatg agggaactaa agcagatata tatggaaaac attcggtcaa
     3421 tggatttact gttggagttg gttatcgttt ttaatcatta gacgggactg gataattgca
     3481 cttgcgtagt gaagtttact aaatgtttgt attttaatga agtattcgac atattccctt
     3541 cagacggaat atgtcgacgg ttatggtttt actggagcat gagtatgtta tggcgaaact
     3601 gataaatata tcagcgtcca ctgaggagag aatgacgcca ggggagagga gggtggcttc
     3661 acgactggag agctttctga atgatgattg ctttgtctgg tacgatatcc cggtgggacg
     3721 gaaaaaccga catccggatt ttgtcattat tgacccggat aacgggctgg tatttctgga
     3781 ggtcaaggac tggactgtat ccacattacg taaagcaaat caggagcagg ttacactgga
     3841 aaccgacggt cttctgaaat cagaaataaa tccgctggta caggttcgtc gttatgcctg
     3901 tgatacggtt aatgccttac ctgctgaccc gtgcttacgg cagaatgacg gtcagtataa
     3961 ggggcggctg aacctggcct gggcgtatgg tgtggtgttt acccgcatta cccgtcagca
     4021 actgaaggca ctgacaggga ataatgagaa tgcggtggaa aagatttttc cttcagcaca
     4081 gacaatctgt caggatgaaa tgacgcagtc tgttttacct gaggtattcc ggcaaaaaat
     4141 tgccgggatg tttaccacgg ggttcagaac ccgggtaaca ccccggatga gggatattct
     4201 gcgcgcccat ctgtttcctg aagtgaccgt gaaacagaac tcacaaatca aaattatgga
     4261 tattcagcag gagattctgg ccagaaatat tggtgacggg catcgtgtca ttcatggtgt
     4321 ggccggttcc ggaaaaacca tgattctttt gttccgttgc ctttacctgg cagaaaccac
     4381 gtccggtagt tacgccgccc cagtgcaact acccggaggg cgttttcaca caggttattg
     4441 tcgatctcca cccagccatt gcggcagtat tcgttcagag cgtcccattg cttcagcaga
     4501 taggtgaacg ctttcgctgt atccgagtgg cgcgacagcg tgttcatctg cccctggagc
     4561 cactcataca acgactgcat tagcggtacc gttctggctt ttctgaccgc cagtcgctct
     4621 tctgccggac tgccgcggat ctcagcctcg atagcgtaca gttccccgat acgacggagc
     4681 gcttctcccg ttactgtcgt tggatggcgg acatgtacat cgtggatctt gcgtcgggca
     4741 tgtgccatac aagccgcttc ggttgcctga ccgctttcgt aaagagcatt gtaacccgca
     4801 tatgcatcgg cctgcaggat acctctgtag tccgccagat gttgctgtgg gtggatgcct
     4861 ttgcggtcgg gagagtatga gaaccacacc gctgccggca tggtcgaacc ggcgttgcga
     4921 tcgtcacgaa catagaccca cagccgtccg gtacgggttt taccctgacc cggctccagt
     4981 acatttaccg gcgtgtcatc ggcatgcact ttacccggca tcagcacata gtgcttcagt
     5041 tcatcataca gcgggcgaag ctgctctccc atgatgtcaa cccagcgccc catcgtattg
     5101 cggtgcagct ccacgccctg gcgggcatag atttccgact gacggtacag cggcagatgc
     5161 tcggcgaact tagccatgat tatgcgggcc agcagagccg gactggcgta actgcgctcg
     5221 atgggttttg gtggctgcgg agcctgaact atacagtcgc accggctgca ggcccgtttt
     5281 gggcgaaccg tttcgattac cctgaacgcg gtgttgatga tatccagttg ttcagagatg
     5341 ctttctccca gcggtttcag tttaccgctg cagacggggc attcggtttc tgccggggag
     5401 ataacctgcc tgtcacgggg aagtgttgcc ggaagtgctt tgcggacggg agagtctgat
     5461 gttttcggcg ctgtctctcc ggccattgag gtgagttgca actgcgcctc accaagcctg
     5521 ttctggagct cggttatacg cgtttctgcc cgtgcgatct tcttttctat cttctcgcgg
     5581 cttttctcgc tgctgcgacc gaacaacatt ctctgcagtt tagcgaccag cgctctgagt
     5641 gagctgatct cgcgggcata gccggttatt tcaccagaca gacggacgat aacagcctgc
     5701 tgtgcgatca gcagggcctt cagttgctcg atgttgtcgg ggagtgtgtt gttcattccc
     5761 ctgttttatc acgggttata tccggatgcc aggccgttct gtccgtttgg gatgttgcca
     5821 tgcgatcccc tccagtagca tggataactg agctggcgtc aggtgcactt tcccttcccg
     5881 ggttaccggc cagacgaagc ggccccgttc caggcgtttg gcgaacaggc ataacccgtc
     5941 acgatcggcc cacagtattt tcaccatttt gccactgcgg ccccggaaga cgaagatatg
     6001 cccggagaac ggggcatctt tcagcgtgtt ctgcaccttc gaagccaggc cgttgaagcc
     6061 acaacgcata tctgtgatgc cagcgatgat ccagattctg gtaccggttg gcagcgttat
     6121 catcgggtac ctcctttcat ttcgcggatt agcgcccgta acatttccgg agtgagaggg
     6181 tcaaacagtt ttaccacacc tgatttaaga tgcagctcgc accgtgggac gtttccggga
     6241 tcactctcag ggcactcatc aggcttgtta cgccagaagg gatttgtaac tggtctggtc
     6301 ggctctggcg tatcagtcag agccaccggg acaggcatgc attcctgtat gtcatcatcg
     6361 ctcagtaagc cgtcctcgta ctggcttttc cattttaaca gcaggttatc attgataccg
     6421 tgctctctgg cgatccgggc aacaacagca ccgggctgta atgcctgttt taccagcctg
     6481 attttaaatt cacgactgta ggaggttcgt cgttcttttc gccatgtgcc ttcgctgatt
     6541 tgaggctctg ttaattcctt ctttctgttg gcataaagga tggcgtcaag ctgagctaat
     6601 gaaactgaat cgggcaatgg ccatgcgata ccggatgcaa taaatcgctg aaaaagcgta
     6661 tgtattgtgg aatgactgag acctagacgc tgagcgatgg cccggatggt cagtttatct
     6721 tcaaatctta aacgcagagc atcaggcaaa taagaacgga agcagggaat atcttttttt
     6781 gtctgggaat tcatcgttcg tgtccatcta tatagatggg cgcgattgtt gccagacagg
     6841 acaattttca caagacgtcg cagatggggc gcttacacaa caatggcgcg agggaaagct
     6901 gctattacct tcttcagaga gcccccagct acttcctgtg actctcgatg cagctgccga
     6961 acagccagaa tcgctcgcag aggacccgga aaccctcagt atcagctgtt gtcaacgacg
     7021 gatgactatt gcac
//
LOCUS       DAZFDC010000187         6999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFDC010000187 DAZFDC010000000
VERSION     DAZFDC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.187"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            60..356
                     /locus_tag="ACHJ48_004409"
     CDS             60..356
                     /locus_tag="ACHJ48_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4709823.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            664..780
                     /locus_tag="ACHJ48_004410"
     CDS             664..780
                     /locus_tag="ACHJ48_004410"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709824.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            999..2384
                     /locus_tag="ACHJ48_004411"
     CDS             999..2384
                     /locus_tag="ACHJ48_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO4709825.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIMIN
                     ELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGPLRHCREYAAKNAEYMA
                     RLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPLTFDNSALTVPVRLIPE
                     ELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWLLDLLYEQNRVVPVIEG
                     KTLCVAPNFDL"
     gene            3205..3768
                     /locus_tag="ACHJ48_004412"
     CDS             3205..3768
                     /locus_tag="ACHJ48_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO4709826.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            3923..6283
                     /locus_tag="ACHJ48_004413"
     CDS             3923..6283
                     /locus_tag="ACHJ48_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO4709827.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(6445..6621)
                     /locus_tag="ACHJ48_004414"
     CDS             complement(6445..6621)
                     /locus_tag="ACHJ48_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709828.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(6759..6999)
                     /locus_tag="ACHJ48_004415"
                     /pseudo
     CDS             complement(6759..6999)
                     /locus_tag="ACHJ48_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1932 a         1363 c         1704 g         2000 t
ORIGIN      
        1 acaaatttac ccggggaata caaactgtaa cgacagttca ccacactatg ggggataaaa
       61 tgccagatca atccggacac tgcaggactc cttcacgcat cttacggatg cctgaactga
      121 gccaattgtt aggtatatct cgttcgacaa tttacgaaaa aatgaatccg ttatcgaaat
      181 actacgatgc aaccttcccc aggccggtac gtcttggtag tggttctgtg ggctggcgtt
      241 catcagctat tgacgaatgg cttactttac atactgtacc tgccagatca gcagtcaaag
      301 gtgttaatga cgaagtgaga catacgcttt cttcagatat gaccatgagc agataagttt
      361 ttgtcatcac gtgatcacct caggaggaaa aagaagcata agaacaattt agccagaagt
      421 ttaattgata aacagaattt ccagcattca ttgttgaaag agcagctatt gtcgctattg
      481 tagcggtaat tgctgctata accgaaacgt attacgaaaa aggaagcatt tatgccttac
      541 atcaaaccgg atatgtcgta ttcagtatat gcatgaccat caatgatagg gaaggcaata
      601 attaaagctc aatacaataa acaagtacct ctgactctgg ttgcaatgtc agtattaaca
      661 gcgatgtcaa ttgcctgtca gaatcaggtt gacgtatgca gtcccggaaa tttacgtggg
      721 gccgttaata tttacactat ggttctggag aaagggaaaa gaccggggga aaggtattag
      781 tgaaataatt tccggaggta ttaagaatga gaaatatgaa tgatatttcg tggtgaataa
      841 tacagtcttc actgaattaa ccttatcggt ttattaaaca tcagatctac aaatgctctg
      901 tatttctcag agcatcaggt ctgaatagca gctattttcg ctattgtagc gggatttgct
      961 gctattttac taacccatta atgaaaagag aggcttttat gtgcttactt gcaccagaaa
     1021 atccttatcc gatatatgca ctaccaccgc tggtgagaaa tgcaataatt gaaactcaaa
     1081 aaaatacaca ggcacctttg gctatggtgg caacatccgc attaacagcg atctcaattg
     1141 cctgtcagaa tcagattgac gtgtgcagac ctggaaattt acatgggcct gttaatcttt
     1201 actctctgat tctggctgat tccggtgaaa ggaaaacgac cgtggataaa gtgtttatga
     1261 aagcatttta tctcagggat gaagccctgg cggatgaata cgcgaaactg gttgagaatt
     1321 acagtacaga aaaggaaata tgggagcaaa aacaaaaagc gctggaatca aaatttcata
     1381 aagagattcg tgccggtaaa gattacaagg caacagaatc agagcttgaa acgcatctga
     1441 ataaacctcc tgttccgccg cagatacgtc gaacaatttt taatgagaca acgatagagg
     1501 gaatgttaaa atattactcc gatagcaatc gctcttttgc tcttgtatcc agtgaagggg
     1561 gggtaatttt tgacagccgg gccatgagta aactgggaat tattaacact ttgtgggatg
     1621 gaggttctct tttcatcgac aggaaatcat ctcccggaat taatttgaag gagccaagac
     1681 tgacgatatc ggtgatgatt cagcctgatg tttaccacaa aggtttttgt acgcgaaaaa
     1741 aagaaattgt gaaaacgtca ggacatcatg caaggttttt gatgtgtcaa ccaacatcaa
     1801 cgcaggggac aaggataatg atcaatgagc ttattgatga aagtctggcg atgagtggtg
     1861 aacgacgttg ccttcacttt tctcctcagg ctgccagaat ctggacggat tattacaatg
     1921 atgtggaatc gaagctgggg ggattggggc ctttaagaca ttgccgggaa tatgctgcta
     1981 aaaatgcaga gtatatggca agactggcag gacttattta ccattcgagc ggtgaagagg
     2041 gggagatttc cccttacact gcagaaatgg cgagagaatt agcaatatgg tacggtaatg
     2101 agtatgtgcg gttgtctaat ccgttaactt ttgacaactc tgccctgacc gtacctgtgc
     2161 gactcattcc agaggagctt gaacttttca actggataaa aagctattgt attgagaagg
     2221 ggatcctctg tatgaaaaaa aatgacattt tacagcgtgg tccgaaccgt ttccggaaaa
     2281 aggataaaat caactggtta ctggatttac tgtatgaaca aaacagagtt gtaccggtta
     2341 ttgagggaaa aacgttgtgt gtcgcaccta actttgacct ctgaacgact taatcgttga
     2401 gtacatatag tctcctctgc tacttgcgga ggagactaat gtctttacca tgtcagcgtt
     2461 gtattagaaa actctggtac aggtcagtca gtaaatataa ctggtgtaac cactatttac
     2521 tattggatag agtgttgctc aggatattgt cggctttagt ctgaggtgag gtggggcctt
     2581 cattgttttc gtgttcaata aataatttag gttaattagt attattagtg gaagttatgt
     2641 gatctgttat gttgtcttgt acgcacttaa cactggttac gtaccggaac actattagag
     2701 tatgttatgg ctctgttaca gtttataggt atgaaaaaat ataatctttc ctgtatgaaa
     2761 taatattctt atggtgtgtg gggtatggtc tgtataccat catttttatg gtgaagttaa
     2821 atatctgtag aggccttaag ctgggtcgct gtttttactt aatggtacag aagtattaat
     2881 agcggtgttg cctgtggcta cccgatactg gcttttgtga atgtcagatt acttcctgta
     2941 agtgattgaa tggtacttcc ttaaactcaa ttctatgagg tgtgttttta ccattgtaat
     3001 tcgtactggt cctcagttta agtgagtctt actgtactct gaaatattac ctctttggag
     3061 taatgaggta atacgaaaca gttttatcag gttgtctgtg ctgctggtta cctcgtttat
     3121 taatgccgcg gtacggcgtt tctgtactct cagtatttat gtaattccac cttcctgtaa
     3181 cttttataaa gtaaggacta taaaatgtat aatgctaatc ccaattacga aatggacttc
     3241 atgattctga aagacgtgaa tgaacacatg gaaggtctgt ttcaacgttt ttctaaactg
     3301 ttacctttca gaattgattt tgcctacagg aaagacacgc ccagttttgg tcattcctgt
     3361 aggcacagta tgtgtattga aatgcaccgc ttacttagtg aaacccaaac gatgctggcg
     3421 ggatattact gggtaatgga atatacgtca aacaagggac ttcatattca ttttatcggc
     3481 tacctggatg ggcaacgtca caacaaatct tatcggatat ccaggcaact gggagatatc
     3541 tggaggcgga tcacggaagg ggagggttat tttcacctgt gcagggctaa agacaaatac
     3601 ccggtgcgta tcgatcacgt aattcactat agtgataaat ctgcggtaga cgacttacgt
     3661 tacgccctta gctatctggc caaacaggat cagaaagaac acggtattat tctgaggcgt
     3721 agcaggcttc ccgaaaaaag taaccggggc cgaccacgac ataactgaat tacagctgta
     3781 ttaggtatta gaattaagtt tctgtaagtt gttgggaggt gcttgagtta ataggcagaa
     3841 gagggcgggg cgctttgtca tcagtgtagg ggacccgaat ttctgccgtg gtgcctcagt
     3901 ggatcggata caggtaacga atatggttca taaatctgac agtgatgaat tagctgcatt
     3961 gagggcagaa aatgcccgtc tggtctcatt acttgaagct catgggattg aatggcgacg
     4021 taaaccgcag actcctgtgc agcgcgtttc tgtattatcc accgacgaga aggttgcatt
     4081 atttcgtcgg ttgtttcgtg ggcgtgatga tgtatgggca cttagatggg aaagtaaaac
     4141 cagcggcaaa tcagggtact ctcctgcctg cgctaacgaa tggcaggcga gaatatgcgg
     4201 taaaccccgg ataaaatgtg gggactgcgc tcaccgtcag ttgattcctg tatccgatct
     4261 cgtcatctac caccatctgg ccggtaccca tactgtcggg atgtatccgt tgctggaaga
     4321 tgattcctgt tattttctgg cagttgattt tgatgaagct gaatggcaga aggatgcatc
     4381 cgcattcatg cgatcctgtg atgagctggg tgtacctgct gcgctggaaa tatcccgttc
     4441 acgtcagggg gcgcatgtct ggatattttt tgcctcacga gtttcggccc gcgaagctcg
     4501 ccgtctgggg actgctatta ttagctatac gtgtagtcgg acccgacagc tgcgattggg
     4561 gtcttatgac cgattatttc ctaatcagga tactatgcca aaagggggat ttggtaatct
     4621 catagcgtta cctctgcaaa aaagaccgcg tgaattgggg ggaagcgttt ttgttgatat
     4681 gaatctccag ccttatcctg atcagtgggc ttttcttgta tcggtgaccc cgatgaatgt
     4741 gcaggatatt gaaccgacga tattacgggc tacagggagt atccatcctc tggatgtgaa
     4801 ttttatcaac gaagaagacc tgggtacgcc gtgggaagag aaaaaatcat caggaaacag
     4861 actgaatatt tctattgcag aaccgctgaa aatcacgctg gcaaaccaga tctatttcga
     4921 aaaagcgcaa ttacctcagg tgctgattaa ccgacttatt cggctggcag catttccgaa
     4981 ccctgagttt tataaggctc aggcaatgcg tatgtcagtc tggaataagc cccgtgttat
     5041 aggttgtgcg gagaattacc cgcaacacat tgcgttgccc cggggatgtc tggacagcgt
     5101 attatctttc cttagggaca acaatattgc tgcagaatta atcgataaac gatttgccgg
     5161 gacggaatgt aatgccgttt ttatgggaaa cctcagagcg gagcaggaag aggccgtttc
     5221 ggcattactc cgttatgaca ctggtgtgct ttgtgcgcca acggcttttg gtaagacagt
     5281 taccgcagcg gcagtgattg ccaagcggaa agtgaataca ctgatactgg tacaccggac
     5341 tgaattgctg aagcagtggc aggagcgtct cgcggtgttt ctgcaggccg gtgacagtat
     5401 tggtattatc gggggaggta aacataaacc ctgtggcaat attgatattg cggtggtgca
     5461 gtccatatcc agacaaggag aagttgaacc tctggtcagg aattatgggc aaatcattgt
     5521 ggatgagtgc catcatattg gcgcggtttc attttctgcg attctgaagg aaacgaatgc
     5581 cagatatctg cttggcctga cggcaacacc aatccgacgg gatggtctgc atcccattat
     5641 ttttatgtac tgtggtgcca ttcgccatac agcggtccgc ccgaaggaaa gcccacataa
     5701 tctggaggta ctgatccgtt cccgttttac atctggtcat ttaccatcgg atgcgagaat
     5761 ccaggatatt ttcagagaaa ttgctctgga tcatgacaga acggtggcga tagctgaaga
     5821 agccatgaaa gctttcgggc aggggcgaaa agttctggta ctgactgaac gtacagatca
     5881 tctggatgag atagcatcag tgatgaattc actgaaattg tctcccttta ttctccatgg
     5941 tcgactatcg aaaaaaaagc gtgcgatgct gatatccggg ctgaatgctc ttcctcccga
     6001 ttctcctcga attttgttgt caacaggcag acttattggt gagggatttg accaccctcc
     6061 gctggatacg ctgattcttg ccatgcctgt gtcatggaaa gggacattac agcagtatgc
     6121 agggcgtctt cacagagagc ataccggtaa aagcgatgtc aggatcattg attttgtgga
     6181 taccgcgtat cctgtgctgc tcagaatgtg ggataaacgt cagcggggtt ataaagcgat
     6241 ggggtacagg attatagctg acggcgatga atcagtgatt taattttaag gagtacttta
     6301 ccctttttaa ataatttgag ctccccgggt tgttcgggca acaaagagtc ccagtataca
     6361 ggcttcagga atatgttgta gtatcaggct tttcatcgct ttaaaatgac taccggcagt
     6421 gagaacgtca tcaaaaataa caatctaggg cctgcaccct tccagtaatt gttcatccag
     6481 ttggtagttt gccataatcg cttctggtaa gggccggttt gttgcggtat gagaggctat
     6541 tgttgatacg ttttgcacaa ccagttccca gaaatccagc catggttcgt aggtttgcgc
     6601 aattcgcaga atttctgtca tacggtcatc gtaagcggca tctgtacggc atttggatgg
     6661 tggtacggga acaaatgtgt attcctcaag attaccgata tttgatatga gcatctgagc
     6721 aacatactca atagcagcct ttttatagtg gtaatcaggc tacgaaagta cagcgtttta
     6781 taaattgtct caggtgatat tcgcagcgtt ttttgacgtg gttttgttcg ccttaaccat
     6841 cctgatattt gctctggaga ccatttcatc tccagctttt ccagaacaag ctttcgcaat
     6901 ggtaaatttt gatccagtaa gcacggtttt ggccttttcg ccattctgtt ggctcggtta
     6961 ttagcatcaa cagctttgta atagcgtctg ccccgatta
//
LOCUS       DAZFDC010000188         6938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFDC010000188 DAZFDC010000000
VERSION     DAZFDC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.188"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..598)
                     /gene="yeeO"
                     /locus_tag="ACHJ48_004416"
     CDS             complement(<1..598)
                     /gene="yeeO"
                     /locus_tag="ACHJ48_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO4709829.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     FAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGS"
     gene            759..834
                     /locus_tag="ACHJ48_004417"
     tRNA            759..834
                     /locus_tag="ACHJ48_004417"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:792..794,aa:Asn,seq:gtt)
     gene            complement(872..1822)
                     /gene="cbl"
                     /locus_tag="ACHJ48_004418"
     CDS             complement(872..1822)
                     /gene="cbl"
                     /locus_tag="ACHJ48_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO4709830.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIILSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1924..2841)
                     /gene="nac"
                     /locus_tag="ACHJ48_004419"
     CDS             complement(1924..2841)
                     /gene="nac"
                     /locus_tag="ACHJ48_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO4709831.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3167..3242
                     /locus_tag="ACHJ48_004420"
     tRNA            3167..3242
                     /locus_tag="ACHJ48_004420"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3200..3202,aa:Asn,seq:gtt)
     gene            complement(3298..4233)
                     /gene="ldtA"
                     /locus_tag="ACHJ48_004421"
     CDS             complement(3298..4233)
                     /gene="ldtA"
                     /locus_tag="ACHJ48_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO4709832.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPVTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4295..5374)
                     /gene="cobT"
                     /locus_tag="ACHJ48_004422"
     CDS             complement(4295..5374)
                     /gene="cobT"
                     /locus_tag="ACHJ48_004422"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO4709833.1"
                     /translation="MQTLANLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALGV
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVNVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASKIVLPGNTTSDLNS"
     gene            complement(5386..6129)
                     /gene="cobS"
                     /locus_tag="ACHJ48_004423"
     CDS             complement(5386..6129)
                     /gene="cobS"
                     /locus_tag="ACHJ48_004423"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO4709834.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6126..6671)
                     /gene="cobU"
                     /locus_tag="ACHJ48_004424"
     CDS             complement(6126..6671)
                     /gene="cobU"
                     /locus_tag="ACHJ48_004424"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO4709835.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6823..>6938)
                     /locus_tag="ACHJ48_004425"
     CDS             complement(6823..>6938)
                     /locus_tag="ACHJ48_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO4709836.1"
                     /translation="VPAVALIHYLNEMCQSTETAVNDAFCRTLTRESLAIP"
BASE COUNT         1869 a         1878 c         1527 g         1664 t
ORIGIN      
        1 ggctaccgtt aatcagtagc ggtattttcg tattccctgc accacgaagt gccccgctac
       61 caattagagt gatggcagct gctggataac tgagtaccgt cagctccaga taagtcaacg
      121 ccagtgcttt aacttctgtc gtggcatcac ccgcgacgaa atcaataatt tgctcgccaa
      181 aatgatgaat aagcgttgcc aacagtacgg caaacaacgt catgatcacc aatgactgcc
      241 gcgtcgccac cctcgctcgt cgtcgatccc gcttaccgag actaaatgcc acaacgacag
      301 tagtaccaag atcgatagca gcaaaaaaag ccataatgac catattgaag ctgtccgcca
      361 atcccacgcc ggccatcgca tcttttccca gccagctgac cagaaaagtg ctcagaaccc
      421 ccatcaacag gacacaggca ttctccatga agataggaac agcaaacggg gttatctcgc
      481 gccagaacaa cactttgtag ctcttgcgtt tagcgtgcca gcgagtgccg tgaacaacct
      541 ggcgtaaagc agaggagata ttcaaagccg accttaattg cagaaagtga aaccacattt
      601 caaataatga gggagaatca gcaaagctgc aaagattttc gccatcaaat tgtctgcaaa
      661 tgcaacaaac tgttgataga aacggcaaac agttgggaaa attaaaaatc gggtttgaca
      721 aaagattttt cgccgttaag atgtgcctca acagcgattc ctctgtagtt cagtcggtag
      781 aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaatttc
      841 tgaaaagccc gcttttatag cgggcttttt gctatatctg ataatcaatt tcctcttcac
      901 tgttttccat cacctgccgc ttgatatcct caactgaaag tcctgcattg caaagttcca
      961 gaaagcgcca gacatagtta cgctgaagtt gtcctcgctt cagtcccaac cagacagtat
     1021 tagcatcgaa aagatgccgc gtatccaggc ggattaaatt cttttcctct tgttcaccgc
     1081 tggattgttc ggcaactaat ccgatcccaa gcccaagagc aacataggtt ttaatgacat
     1141 cagaatcctg cgcacttaat ataatatctg ccagcaaacc tttgcgggca aatgcgtcat
     1201 caatacgtga gcgccccgta atcccctgtc ggtaagtgat taacggccac ttcgctattg
     1261 attccagcgt caatggtgta atttgcgtca agggatgatc gtgtggaaca agcaaactat
     1321 ggtgccaacg aaaccacggg aaggcgacga gctgcgggtc attactcaaa cgctcgctgg
     1381 cgataccaat atcagcttca ccattatgca acaacgtcgc aatttcctgt ggcgtcccct
     1441 ggattagctc gagccgaacc tccgggaaaa gttcgcgaaa agctttaatg acctctggca
     1501 agctataacg tgcctgagta tgcgtcgttg caatagtgag aacgccagac gtatcgttgg
     1561 taaacaggtc tgcaagccga cgaacattac tggcttcatt gagaatacgt tctgcaatga
     1621 ccagtaatgc tttgcccggt tcagtcatgc ccagcagtcg cttacctcgt cgaacaaata
     1681 tttcgatgcc aagttcatcc tccagttccc gaatatgacg gctgacgcct gactgtgagg
     1741 taaaaagcat attcgcaacc tctgtcaggt tgtaatcctg acgtgcagct tcgcggatta
     1801 tctttagttg ttggaaattc acggtaaact ccgggcagtt cagatatccc gttattgtta
     1861 aagtctaacg cccggcataa caaataataa aaacccgcat cttattccat cccgatataa
     1921 cgcttagctc accaattgcc actgcctttt ttccatcact ggagaactaa tcactgacat
     1981 taacaactct ttcactgcct gtgcctgtgg cgataagttc gctctggcgg gtaaatttaa
     2041 tgacaaagag agactcatgg aaggcgtggt aatgcgtgac atccacccat ttactgcgcc
     2101 acataacgaa cgcgcggccg attcgggtaa tactgcaacg cccatgccgc tggcaatcgc
     2161 tgcggtaagc gtagcaatag actcaatttc accaatgact tttgccgtga gccgccgtag
     2221 ggaaaacgcc tcatcaacac gaagtctaac agcgctgtaa tcactgggga gaaaaaggtt
     2281 catttgtgca atagcattaa catcaacgct ttgccccggg caatcttgag ttcctaccag
     2341 gaaaagatct tctttcagca aagcctgact ggatacacca gccacagggg aatgctcata
     2401 aatcaccgcc atatcgagtt ggtgatttat cagtttttcg ttaagcactg caccgctatt
     2461 ttcatgaaga tagataacga cctccgggaa ttcagcgcga accgcctgta ataagggcat
     2521 ggtgatggat gacgcagcgg ttcctggcgc aaagccaatc gagacttgcc ccgataatgc
     2581 ctgaccaacg ttatgcaccg ccagttgggc ctgctcacac tgacgtaaaa tggcccgcgc
     2641 atgggtatag agaatttttc cggcgtctgt tggcgtaacg ccccgttttg tgcgaatcaa
     2701 aagttgttga tttaactcac cttcgagtgt ggcaacctgc tggctgagcg ctggttgtgc
     2761 gatatgcaat acttcagcag cctgagtcag gctaccaata tctacaattt ttacgaagta
     2821 tttcaggcgt ctgaagttca tgttgcctcc ggtttttgag aatcggccca agtgccgcca
     2881 ttacttacaa ccagattgca agatgcttgc cagttttatt ttggcgttga tgtacaagct
     2941 aaccaactgt caaataagag attatgatag attcgtcatt ggctcttttc accagcagtc
     3001 gcgttcccct gccctataaa aggagggtat gcaccacgat ggttcattac caataagctt
     3061 gaaatctcat cactttgttg aaattgacag caaacaaaca aaaaaatgca tttcaccctt
     3121 tgacatcacc atgcactgcc attaatatgc gccccgttca cacgattcct ctgtagttca
     3181 gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag tcagaggagc
     3241 caaattcaaa aaagcctgct ttctagcagg ctttttgctt tctaattacc aacgctctta
     3301 aaacatctgt cttgaaccag aactaatttg cacaggcatt cccgatcgac gttgcaacgc
     3361 agcatttgcg cgattcacat caacttcttg cccgttgata aacgcccgca aagatggggt
     3421 taccggcaat ggcacttttc ggtcagactc atattcagca cgattgcgcg acagcggctc
     3481 atgaacttcc agccactttg aaccatccgg ttccgttgtg tatttcactg gctgatcaat
     3541 aatttgcaca cgagtaccta caggaacatt atcaaacaga tatttgatat cgtcattgcg
     3601 cagacgaata cagccctgac ttacccggag cccaatacca aaattggcat tggtaccgtg
     3661 gatggcatat agcctgccaa tataaatcgc gtacagcccc atgggattat cgggccccgc
     3721 aggaacaaat gcgggcaaac tctcccctcg tttcgcatat tcgcgccggg tgttcggcgt
     3781 tggcgtccag gttggtgctt cttgtttacg ttcaacggta gtcacccagt tacgcggggt
     3841 ttctcgccca gcctggccga taccaatagg aaagacttcc acagtattac tgtctggtgg
     3901 gtagtaataa agacgcatct cagcgacgtt aaccacaatc cctttacgaa cagtgacggg
     3961 caaaatcagt tgctgcggaa tggtgagttg cgaacctgac ttcggtaaga agacatcagc
     4021 gcccggattc gcttccagca tgttacttaa cccttgcccg tattgtgcgg caaaagtctc
     4081 cagcggctgg gtattgtgat caggaacagt tacagtaagc gactgcccca ctaaacggct
     4141 accctctgga ggtaatggat aagttaccgc caggctagta tggctggcaa aaagcagagc
     4201 aaatgagcaa agaatattta cacgacgcat catgtccctt tcctaagtcg cgaaagctat
     4261 cggttaagta tagcttttat cagacttttc gtttttaact gttcaaatca gaagtcgtat
     4321 tccccggtag aacaatttta ctggcagcaa gttcgcccat gttgttgtat atcgcacaag
     4381 cagcttcgat gatgggcatc gccagagctg cgccactccc ctcacctaaa cgcatatcca
     4441 tattgagaaa aggctccagc cccaaatgcg acagcgctat acgcgcccct ttttctgccg
     4501 acaggtgaga aggaatgaga tacggtttga ttgcaggaga catctggcag gctgcgagcg
     4561 cagcagcata agaaagaaat ccatccagca acacgggtaa accgcaggaa gcagcgccta
     4621 acatcacgcc agccattccg accaaatcaa atccacccac ttttgccaga acattaacgc
     4681 catcctgcgg atttggctga ttcaacgtaa tcgcccggcg cacaacatca attttattag
     4741 ccagtttatc tgtcggcagg tttgcgccaa tcccaaccac ttcttcagga tcccggccag
     4801 tgattgtgct gactattgct gctgccggtg tcgtgtttgc catccccagt tcacctacac
     4861 caaacagcgt gacaccgttt tttgccagct cccgcgtata acatatgacg tccaaaagta
     4921 acttttcagc ctgacggcga ctcattgccg gagctgaagc aatattgccg ctacctcgtg
     4981 cgacacgcat gttgataagc ccggggatag gctcagcagt atcaatacca acatcaatta
     5041 cgtggacgtt agcgcccgct tgtgctgcca gcacacacac gccggttgtt ccgcgggtca
     5101 tattttcagc ctgaatggct gtcacttctt ttggggaaat agcgacccct tcctcccaga
     5161 cgccgtgatc ggcacacata accagtaccg cttttttgcc cacatgcggt atgccattca
     5221 accccggcat tcccgccagt tgtacgccaa gcgcctccag ccttcccagg ctaccaacag
     5281 gtttgagtaa cccgtcaata tgccgttgtg cacgcgacat agcggcagga tcgatggcag
     5341 ggatcgtatt cagtaaattg gcaagtgttt gcatgtcacg tctctttata gcagtgccag
     5401 caggaagacc agttcaccaa gctctatggc tgctcccagc gtatcgccgg tttgcccgcc
     5461 cagcgtacgt ttgagaagct gaccgaggat gaaaatcgcc accatcgtca ccaccatagc
     5521 agccacacca tgcatacccg gcaataatac ggcagcaaaa atagcggcta agccgagtgt
     5581 gacgcaggtt tgtcgcccat caattttgcc aatgaacaca ttgccaagcc cttcttcccg
     5641 ggcataacga tgacgataca tcaataatgc ggcagtcccg cggctgaccg cacatgctgc
     5701 cgccagcgat gcaaggatcg gttcgccacg cagagccaac tcgcttaaca ccaaaatctt
     5761 tgccagtaca acaaaaatca atgccagacc gccgtgggta cctaaacggc tatcacgcat
     5821 gatttccaac atgcgatcgc ggctacgtgc agaaaatacg ccatcacagg tatcagcgag
     5881 gccgtccaga tggaatcccc cggtcatcag caccagcaca agtacgctaa acagtgccgc
     5941 cagtggtgcg ccacaccatg cctgcagcac catgaagacc agcccgctaa tcgcgccaag
     6001 caataatcca atcaaaggaa aagtaataat accgcgagaa taatgctcga aatccagtcc
     6061 ctgggaccag cgacgcggta caggcagacg cgtaataaat gagagcatcg cccaaaataa
     6121 tttactcatt tgatttttac tccaataccc gaaaccacca gccatacttc atttgccgca
     6181 gcggccaact gctgatttac ccgcccggca atatcacgaa aatgtcgtgc cagacgactc
     6241 tccggcacaa tccccattcc cacttcgtta gtcactaata caacctttgc ggggcaacgt
     6301 tggcaggcag caatcaacga ctgaatctca gcattaatcg cctgttccat cgcctgataa
     6361 tcccattcat cagggtcttt atcgccgcca taatcaaaca acagattagt caccattgtg
     6421 gtaacgcatt caagcaacac aacctcattc gggttaatgt ctgcatgaat taattcatca
     6481 agatgttgcc agcgctccac tgtgcgccag tgctccgggc ggccttgccg atgatgttct
     6541 atccgtgcag ccatctcatc atcaaggatt tgcgaggtag cgatatacag aacctgtgaa
     6601 gagtccccaa taagcgcctc tgcgtggcga ctcttcccgc tccgtgcgcc ccccgtcacc
     6661 aaaatcatca taccgactcc tgattcaaat gtatgatggt attcattgaa cagaacaatg
     6721 gatcccccac cgttgctgac cgcaaaacat ttacgcgatt aatactgcgc gtaatataat
     6781 taaaaatcca gcattctcaa tccattacga aataagatgg cattacggaa tagccagact
     6841 ttctcgtgtt aacgtccgac aaaatgcgtc attgacagcg gtttctgtgg attgacacat
     6901 ttcattcaga taatgaatta atgctactgc cggaacaa
//
LOCUS       DAZFDC010000189         6835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFDC010000189 DAZFDC010000000
VERSION     DAZFDC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.189"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1941
                     /gene="espL4"
                     /locus_tag="ACHJ48_004426"
     CDS             <1..1941
                     /gene="espL4"
                     /locus_tag="ACHJ48_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO4709837.1"
                     /translation="EITIINKNENTTLTQTIAPIFEEYLMEILPQRSDALDKKELNLN
                     SDRKEKEFPRVKLNGQCYFPGRPQNRIVCRHIAAQYINDIYQNVDYKPHQDDYSSAEK
                     FLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKMESNDLSVMAAILLVD
                     NHALTVRLRIKNTTEGCIHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYS
                     LLKWSNDHVINQSVAIIPALPKEQLLMLKGTVDEITPPLSPATMNLLMAIGQNHQLKQ
                     LMIQLQKMPELHRTEMLTAYNSINLPGLYLAINYGNADIVETIFNSLSEPGYEGLLSK
                     KNLMHILEAKDKNGFSGLFLAISRKDKNVVTSILNALPKLAATHHLDNEQVYKFLSAK
                     NSTSSHVLYHVMANGDADMLKIVLDALSLLIRTCHLTKEQVLDLLKAKDFYGCPGLYL
                     AMQNGHSDIVKVILEALPSLAQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVI
                     NTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVEMVYLALSDH
                     ARLFGFTAEDIMDFWQHKAPQKYSAFELACELGHRVIAELIFNTLNKMAESFGFTDNP
                     RYIAEKNYMEALLKKASPHTVR"
     gene            complement(1910..3646)
                     /gene="aceK"
                     /locus_tag="ACHJ48_004427"
     CDS             complement(1910..3646)
                     /gene="aceK"
                     /locus_tag="ACHJ48_004427"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO4709838.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLHWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3830..5134)
                     /gene="aceA"
                     /locus_tag="ACHJ48_004428"
     CDS             complement(3830..5134)
                     /gene="aceA"
                     /locus_tag="ACHJ48_004428"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO4709839.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5164..6765)
                     /gene="aceB"
                     /locus_tag="ACHJ48_004429"
     CDS             complement(5164..6765)
                     /gene="aceB"
                     /locus_tag="ACHJ48_004429"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO4709840.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGSVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1747 a         1709 c         1636 g         1743 t
ORIGIN      
        1 gaaattacga taattaataa aaatgaaaac acgacactca ctcaaaccat tgctccaata
       61 tttgaagaat acctaatgga aattttacct caacgctcag acgctcttga taaaaaagaa
      121 ttaaatctaa actcagatag aaaagaaaaa gaattcccaa gagttaagct taatggtcaa
      181 tgttattttc cggggcgccc ccaaaaccgt atagtatgcc gacacattgc tgcacaatat
      241 attaatgata tttatcagaa tgttgactac aaaccccatc aagatgatta ctcttcagct
      301 gaaaaatttc tcactcactt caacaaaaaa tgcaaaaacc agactttggc gttgatttcc
      361 agccgtcctg aggggcgttg cgttgctgcc tgcggtgatt tcgggctagt tatgaaggca
      421 tattttgaca agatggaatc aaatgacctc agtgttatgg ctgccatatt attggtagat
      481 aaccatgctt tgacggtccg gctaagaata aagaacacaa ctgaaggatg tatccattac
      541 gtggtttcgg tttatgatcc taatgtaact aacgataaaa taagaatcat gagcgaaagt
      601 aaagaggata ttaaacacta ttcactgatg gattttatga atgtagatta tagcctcctg
      661 aaatggtcaa atgatcatgt tattaaccaa tctgttgcaa taattccagc acttccaaaa
      721 gaacagctat tgatgctaaa aggaactgtg gatgaaataa cccctccatt atcacctgca
      781 acgatgaatt tgctaatggc aattggtcag aatcaccaac ttaagcaact gatgattcaa
      841 ctccagaaaa tgccagaact acatagaaca gaaatgttga ctgcctataa tagtattaac
      901 ctccccggtt tatatttggc tataaattat ggtaatgcgg atatcgttga gactattttt
      961 aattcattgt cagagccagg atatgaagga ttactctcga aaaaaaatct catgcatatt
     1021 ctggaggcaa aagataaaaa tggtttttct ggattatttt tagcgatatc acgtaaggat
     1081 aaaaatgttg taacctcgat tctgaacgcc ttacctaaac tggccgcaac acatcattta
     1141 gataacgaac aagtgtataa attcctgagt gccaaaaata gtacgtccag ccatgtttta
     1201 taccatgtta tggcgaatgg tgatgccgac atgctgaaaa ttgttttgga cgcgttatct
     1261 ttgttaattc gcacatgtca tttgactaaa gaacaggtac tcgatctcct gaaggcaaag
     1321 gatttttatg gttgcccagg actatacctg gcgatgcaaa atggacatag cgatatcgtg
     1381 aaagttattc ttgaagcatt gcccagcctg gcccaggaaa ttaacatttc agcttccgat
     1441 attgtagatc ttctgaccgc taaaagtctt gcgcgcgaca cgggtttgtt tatggccatg
     1501 cagcgcggac atatgaacgt tattaatact atttttaacg cattacccac tctgtttaat
     1561 acgtttaaat tcgataaaaa aaatatgaag cccctcctcc tggcaaataa ttctaatgaa
     1621 tacccaggtt tgttttcagc gatacagcat aaacagcaaa acgttgtaga gatggtttat
     1681 cttgctttat ctgaccatgc acgcctgttt ggatttaccg ctgaagatat tatggatttt
     1741 tggcaacaca aagcgccaca aaaatactct gcctttgagt tggcttgtga attgggtcac
     1801 cgggttattg ctgaattaat ctttaataca ttaaataaga tggctgaaag ctttggcttt
     1861 acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact caaaaaagca
     1921 tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca tcttccacat
     1981 gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt
     2041 gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccaatgg cgaaactctt
     2101 ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt tcgtcttccg
     2161 ggtagcgcgg cggcgggata tcgcggaaat tcacttccgt catgtagcaa atttcatcgt
     2221 aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc
     2281 ccgggaaaat gttagcagcg gcaagctggc gaatggcatt gccgtactct tcaatggcgt
     2341 cgcgcagctg ctgaccttcc acttgttcca gccagatatt gagcggcacc atccgccgct
     2401 caatataaag atggcgaatc acaatttgtt cgccgagatc ggtgattttt tccgctgctt
     2461 cctggagcag taattccatt aatgccgggg aaatatgccg cttctccagc acaaagtttt
     2521 caaactcctg ggtatccgcc attcggccca cgcgatcgtg ctctttcacc agttgatagc
     2581 aggcacgaac gtgagcggca gacatctctt tctgcggcgc gaacttgtct ttgatgactt
     2641 taaatacacg gtcaaagccc ggcagcgtga acaccaacat caccatgcca cgaatacccg
     2701 gcgcttcaat gaactgctca ttacagccct gtagataaac gagatattcg cggtagcttt
     2761 cggttttggc gtgcttctgg cagccgatag ccatatacaa ttcagcggtg gttttacctg
     2821 gcagaatttc ccgtaaccac tcgaccagcg ctgcgggcag cggcgcataa accataaaat
     2881 aagaacgcgc aaagccaaaa acaatgctcg cttcggcggt cgtcgtcaga caggtatcaa
     2941 taaacagttc gccgtcatcc gtctggtgga tcggcaataa aaatggcaat gtgccggaag
     3001 gcgttattag tttgcccacc agccaggcgg ctttattacg gtaaaacagt tcgttcgcca
     3061 cctgtaaatg acttttggag aggttttctg gccccaacgt ttccgtcaga tggcgaataa
     3121 tgtaatggat gtcacggctt ttattctgcc agtgcagacg caacggcagg tcgctgataa
     3181 cgcgcatcag cagagattcc cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg
     3241 gaatggtacg aaagcggcgc tctggctggg agctaaagat aaaaagccgc tcgggagtaa
     3301 gcgagcggtg gtcaaataac cgacagtaca cggagttaaa aaagctctcc gcaatctcga
     3361 agcgcgggta atccggcaac agccgggtgt aatgctcttt gacgcgcagt aaaaattccg
     3421 cgtccgtgct ttggccgttg gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat
     3481 gatcgtaaag atggatacgg tttttcatcg cctgctggac agcatgccag tcggcttgtt
     3541 cgaaacgctg ctgcgccccg gaagtcactt cgaggaatcg accatactga gcgtcgaagc
     3601 cttgcaaaat ggtttgagca atcagtaatt ccaggccacg cggcatttag cgccctcatc
     3661 aggagcagag aattgccgga tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg
     3721 ttgtaggcat gataagacgc gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg
     3781 cggcgtatat gccttatccg gcctacagtc agcaacgttt gtaggttgct tagaactgcg
     3841 attcttcggt ggagccagtc agcgcggtaa ccgacgacgt gccgccctga ataatggtcg
     3901 tcactttatc gaagtaacct gtacccactt cctgctggtg agatacgaag gtgtagccat
     3961 ctttcgcggc ggcgaattcc ggctgctgca ctttctcaac atagtgcttc ataccctcgc
     4021 cctgggcata ggcgtttgcc agatcaaaca tgttgaacca catgctgtgg atacctgcca
     4081 gggtgatgaa ctggaacttg tagcccatat ccgacagctg ctgctggaag ctggcaatag
     4141 tgttgtcgtc gaggtttttc tgccagttga acgacggcga gcagttataa gccagcagtt
     4201 tgcccggata tttcgcgtgg atagcttccg caaagcgacg cgccagttcc agatccggcg
     4261 tggaggtttc acaccatacc aggtcagcat atggcgcata cgccaggcca cggctgatcg
     4321 cttgctcaat gcccgcatga gtacggaaga agccttcact ggtacgctcg ccggtaataa
     4381 attcgctgtc atacgggtcg caatcggagg tgatcagatc cgccgcatca gcatcggtac
     4441 gtgcaaccag cagggttgga acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt
     4501 tctgaatagc ttcctgagtt ggcaccaaaa ctttgccgcc catgtgaccg catttcttca
     4561 ctgacgccag ctgatcttcg aagtgaactg ccgctgcacc ggcttcaatc atcgctttca
     4621 tcagttcaaa ggcattcagg acaccgccaa aaccggcttc cgcgtcggca acgatcggca
     4681 ggaagtaatc gacatagcgc ggatcgcccg gctcaatgcc cgcggaccat tggatctgat
     4741 ccgcacggcg gaaagtgttg ttgatccgct ccaccacagc tggcaccgag tttgccggat
     4801 agagcgactg atccggatac atgctggccg ccaggttagc gtccgccgct acctgccatc
     4861 ccgacagata gaccgcttca atacccgctt tcgcctgttg cagcgcctga ccgccagtca
     4921 gtgcaccgag gctgttgatg tagccttttt tcgactcgcc atgcagcaga cgccacattt
     4981 tcgccgcgcc cagttgcgcc agcgtgcatt caggattgac tgaaccgcgt aatttcacca
     5041 catcttccgc gctgtatggg cgagtaattc cttcccaacg cggttgagtc cactctttct
     5101 gtaattcttc aatttgttgt gtacgggttt tcatgtgcag atgctccata gttatgtggt
     5161 ggtttacgct aacaggcggt agcctggcag ggtcaggaaa tcaattaact catcggaagt
     5221 ggtgatctgt tccatcaagc gtgcggcatc gtcaaaacgc ccctgggaga aacgttcttc
     5281 gcccagttcg ctggcaatga ctttcatctc ttcgcccagc atctggcgga acaaggcttt
     5341 agttaccggt ttgccattgc tcaacgtttt ttgatgatgg atccactgcc agattgaggt
     5401 acgggaaatt tcagccgtcg ccgcatcttc catcaggcca taaatcggca cgcagccgtt
     5461 gccggagatc catgcttcga tgtactgcac agccacgcga atgttggcgc gcataccttc
     5521 ttcggtgcgt tcaccatcac aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg
     5581 ttcgcgcatc acttcaagct gatttttacg ggagccgaga atgtcgttga ataccgccat
     5641 tgccgtgtcc gcaaggcctg ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc
     5701 cagcgattta tccgctttta ctttgttgag cacctggtta ttgtgctctt catctttgct
     5761 cggaataaac gccgccatgc cgcccatcgc aaaagcaccg cgtttatggc aggttttaat
     5821 caacaggcgt gagtaagcat tcaggaatgg tttatccatc gttactgcct gtctgtctgg
     5881 caggacgcga tcgggatagt ttttcaacgt tttgatatag ctgaagatgt aatcccaacg
     5941 accgcagttc agaccaacaa tatggtcacg cagcgcgtga aggatttcat ccatctggaa
     6001 cacggcgggc agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa
     6061 gcgatcttct gcatagctga agacttcgct ccaccaggcc gcttcctgcc aggactgggt
     6121 tttcggcaga tagaaatagg gaccactgcc ctttgccaac agtgcctgat agttgtggaa
     6181 gaaatagagc gcaaaatcaa acaggctgcc ggggattgcc tcaccacgcc aggtgacatg
     6241 tttttccggc aagtgcagac cgcgtacccg acaaatcaaa accgctggat tgggcttgag
     6301 ctggtaaatt ttgcctgctt cattggtgta actgatggtg ccgttaaccg catcacgcag
     6361 gttaatttgc ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc
     6421 cataaagact ttcacattgg cgttgagcgc gttgatcacc atcttgcgct ctacggatcc
     6481 ggtaatctct acgcggcgat cttctaagtc cgcaggaatc ccgcgaattt tccagtcagc
     6541 atcacgaatg gaagctgttt ccgaaataaa atcaggcaac gttccgttat caatatcttg
     6601 ctgctgctga atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag
     6661 ctcagtcaga aattctaccg cttcggcagt aagaatttgc ttctcctgct cgccatacgg
     6721 ccttatgaaa gccagttcat cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt
     6781 catggatccg aaaacttccc cactgtgaac gaaggatcgt tgtgcgcttt tcgtt
//
LOCUS       DAZFDC010000190         6745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFDC010000190 DAZFDC010000000
VERSION     DAZFDC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.190"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(353..625)
                     /locus_tag="ACHJ48_004430"
     CDS             complement(353..625)
                     /locus_tag="ACHJ48_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4709841.1"
                     /translation="MALKCPECGTTAHARTSAYEAPSVKRSWYQCQNLECSYTFTALE
                     SVDTIIMKPRRNEQESEKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
     gene            complement(631..1182)
                     /locus_tag="ACHJ48_004431"
     CDS             complement(631..1182)
                     /locus_tag="ACHJ48_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO4709842.1"
                     /translation="MMARVTPDQALISFRNARILWAGHSESRKAVEQQIESLLTAMEK
                     PADYARQLELQREHLDVLKWQINCAARECIYSQHQLMEACTEDALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRNALVRQLAITPPEIAGDYRMILDESGVMPDPMMVRDCQ
                     SSYTPAQQLRFQQRLDYINGMQE"
     gene            complement(1179..1931)
                     /locus_tag="ACHJ48_004432"
     CDS             complement(1179..1931)
                     /locus_tag="ACHJ48_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO4709843.1"
                     /translation="MTIPEQNNNPIVEISIPVSTQQALEKVNATKSAWLEARRQQKAA
                     ADNIATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFD
                     ELLAAQESENALLPQEAAELAGKYIHAHDTLVGIRAKQIWEDFMQSHGKALIQTLSLL
                     KSTMGREASAVVGVVHSVNDPDTLLKDFIHKHITRPALTNDAMPEQDPVFKLAGVAPD
                     YAARLDFSNKLSPAAMHKMKVRQERAEKEKAV"
     gene            2865..3125
                     /locus_tag="ACHJ48_004433"
     CDS             2865..3125
                     /locus_tag="ACHJ48_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4709844.1"
                     /translation="MRNISVSAPAPAAPLFPVSNHHERFLRLPEVMHLCGLSRSTVYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
     gene            3122..3679
                     /locus_tag="ACHJ48_004434"
     CDS             3122..3679
                     /locus_tag="ACHJ48_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4709845.1"
                     /translation="MMIPAYIKYLYSGLLTVVISRYSFSAVAKSAAGIGVPYNLLATI
                     DAPCVFFYVVAQAQPFSGLWCLCLHHGSIEIMVVRAGQPSGWPVSNKAGYANPVRAAT
                     SEIGVSGGSNNRYLLEAAIMATILTPSHPQYVFVFAAIRRADTHPRICMLRTVSCDER
                     SARRLLVRDYVLSLSARLPAGEVTL"
     gene            3676..3897
                     /locus_tag="ACHJ48_004435"
     CDS             3676..3897
                     /locus_tag="ACHJ48_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709846.1"
                     /translation="MNQFEISYDDVVRLKHLRNVGEYVTGMAVLQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCHDDLMNAEVA"
     gene            3897..4220
                     /locus_tag="ACHJ48_004436"
     CDS             3897..4220
                     /locus_tag="ACHJ48_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO4709847.1"
                     /translation="MICCESLLALRAALYRRAVACAWLALSNHQERYSGLTLAELEDA
                     IARELEGFYLRQHGQQRGLEIACALLSDLMESGPLKACPVLSLLGMTVMDELCSRHLN
                     KPALH"
     gene            4234..6567
                     /locus_tag="ACHJ48_004437"
     CDS             4234..6567
                     /locus_tag="ACHJ48_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO4709848.1"
                     /translation="MSGMKVSQAEKAARGHWSRILPALGVNVLKNRHQPCPVCAGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVSKVLDVGISEAADRINGIIGNLLPVSQGMLES
                     GSPEKEDGRKAAAVLAARLFDKSRQTTGNAYLTSKGFPALPCRELTAMHKVGGVAFRA
                     GDLVVPLYADGELVNLQLINANGGKCFLKGGQVKNAFYLVEGTAKAAKRLWIAEGYAT
                     ALTINYLTGDAVMVAFSSVNFLSLASIACSEYPTHQIIIAADRDLNGAGQTRGAAVTG
                     ACNCTMALPPVFGDWNDAFTQNGEEATRHAIHEVIKPAVASPFDTMSEAEFTALSVSE
                     KAQRVVDHYKNSLAVDPNGQLLSRYEAGAWKVIYYADFARDVAALFQRLDAPFSSAKI
                     ASLVETLKLIVPQQQNPARQLIGFRNGVLDTRTGLFSPHDKKHWLRTLCEVDYTQPVD
                     GESLETHAPAFWRWLDRAAGFNPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIEMLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPDQIAPEERD
                     TQLKEKIASELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADSAFDFCGYL
                     EVLPDTTGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPLMLKEY
                     GLQYEKRRTNQGMQTNLALREESNADWLPKCDEFAAK"
BASE COUNT         1584 a         1630 c         1713 g         1818 t
ORIGIN      
        1 ctcagttcat ttctaaataa tttgcaaaat tattttgggt actttcgcct ctacacttcc
       61 ttcgggtggt tccacaataa tggatctcac cggcgtttga tattttataa tctaacccct
      121 tagcgcgcgc agccccccac gcctgctcgc ttcgcttaac agactggttt tcatgcattc
      181 cgcaaatcgt ctcagaagcc accacacaag ggctttcgcg tcaaaaatgg tgcatgagaa
      241 tcatgcgttt tcatgcgcta tagacatgca ctcatgcgct ctcaggccag ccagggaaaa
      301 ggcgtaaaaa atcccagtac tggaccggga ttacgtgggc tttttttgct aatcagacag
      361 gaatttgctg acggcttgac agctttgacg cggagccata gcgattgaga gtttgctgtt
      421 gtttttccgg catttgtgct ttctctgatt cctgttcatt gcgccggggc ttcatgatta
      481 tcgtatccac actttccagt gcggtaaatg tgtaggaaca ttcaagattc tggcactgat
      541 accatgagcg tttaaccgat ggtgcttcat aggcggaggt tctggcgtgt gccgtcgtac
      601 cgcattcggg acatttaagt gccattaata ttactcctgc atgccgttaa tgtaatccag
      661 acgttgctga aagcgcagtt gttgtgcagg ggtgtaagag ctctggcaat cacgaaccat
      721 catggggtca ggcatcacac cggattcatc cagaatcata cgataatccc cggcaatttc
      781 aggcggtgtt attgccagct ggcgaaccag agcattgcgt aatatacggg acgcaacgtc
      841 aacccctccg cgccccttca ggaacggagc cagtgcagat gtcagggctg ctccattcgc
      901 ctgcataaaa ttgctgagag cgtcttctgt gcaggcctcc atcagttgat gctgcgagta
      961 aatgcattcg cgcgcagcac agttaatttg ccatttcagc acatccagat gttcccgctg
     1021 taattcgagc tgtcgagcat aatctgctgg tttttccatg gcagtgagga gtgattctat
     1081 ctgttgttca acggcttttc ggctctcact atgtccagcc cacagaatac gggcatttct
     1141 aaaactgata agcgcttggt ctggagtgac tcgggccatc atactgcttt ctccttctca
     1201 gcacgttcct gacgaacctt cattttatgc atagctgctg gagagagttt attactgaaa
     1261 tccagacggg ccgcataatc cggggcaact cccgccagtt taaacacggg gtcctgttcg
     1321 ggcatcgcat cgttagtcag agccggtctg gtgatatgtt tatgaataaa atctttcagc
     1381 agtgtgtccg ggtcattaac tgaatgcacc acaccgacaa ctgcgctggc ttccctgccc
     1441 attgttgatt tgagcaggct tagtgtctgg attagtgctt ttccatgcga ctgcataaaa
     1501 tcttcccaga tttgttttgc gcgaataccc acaagggtgt cgtgcgcgtg gatatacttt
     1561 ccggctaatt ccgcagcctc ctgcggcaaa agtgcatttt cgctttcctg agccgccagc
     1621 agctcatcaa aatcctcaag cgtttctcgt cccagcgcaa tttccgtgcg cagttttttc
     1681 atttcctttg agactacgcc ctgactttca cgaaatagcg tgcgccactc ctcattcagg
     1741 gcgttcgtcg tggcttccat ttcagcgcgg cgctggcgga ttgtcgcaat attatccgct
     1801 gccgcttttt gctgacgacg tgcttcaagc catgcagatt tggtggcatt cactttctca
     1861 agcgcctgtt gcgtcgaaac agggatagaa atttcaacga tcgggttatt gttctgttct
     1921 gggattgtca tgtcagcacc tagtggtttg tcgtggtgtc aattgtgctg tgatgcatac
     1981 aaagctgcca ttgaacgcca ttgtgcgagc gacaagacaa agactgaacc gccccggacc
     2041 tcttttggct agccagaaaa ggcctcgttt aactaaggct gtttcagact taccttaact
     2101 attcactact attcacttag gtaaaaaaaa taggtaatac agaaggttat aaggtgaagg
     2161 gttggcatgg cagtattcat cgactgttca taactgttca catgggaata aaattattta
     2221 tggtattttt tcataatggg tggttttttt attcctcgta gctatttgtt ttttgctcct
     2281 caccactatt cacccttatt caggagtatt caacaggcac ataaatagac tgcatgtttt
     2341 tgccggggaa taacattttc gcctctggcc atggtgttgc atttcatact gatttttata
     2401 aaactagatc tgtccggttc tttatggatt cattcggata tattttacga tttaaggtga
     2461 tgttatttaa tatcaaggtt tttatgcagg cccaatgttt ataagttgga tgcgattcaa
     2521 aaatatgccc ggaaattggg gcctgcaatg caaggcctcg tgtagaagtt gtactgcctt
     2581 gctgtattgg cggtggggtt agcactatca ggtgcaatga cccataggat agtaaaccct
     2641 gggtgaatgg tagttcctat taatgctcct gtatttatag tccgcaggca tggtatttat
     2701 tatgtcattt tatatggttt aatcgggctt taaattccat aaaatcaata tgtgacgcta
     2761 aataaaaaat gcgtgcgcag gtggtttata tacacataat aaggaactac ctgaacccgg
     2821 ataaattcat ccggactgtt gtggaaatta aagagggtag ataaatgcgt aatatttctg
     2881 tgtctgcgcc agctcctgct gcgccattat ttcctgtatc gaaccatcat gagcgatttt
     2941 tacgcctgcc tgaggtgatg cacttatgcg ggttgtcccg ttcgaccgtt tacgacctga
     3001 tcagccgcga tgcgtttccg aagcagatcc cgcttggcgg taaaaatgtc gcctgggcac
     3061 agtctgaggt cagcgcatgg atggcggacc gcatcagcgc ccgtgggcgg ggatgtgatg
     3121 catgatgata cctgcataca ttaaatacct ttattctggc ttgcttactg tcgtcatttc
     3181 caggtatagt ttttccgctg tcgcaaaatc ggcagccgga attggcgttc cgtataactt
     3241 attggcgaca attgacgccc cttgcgtctt tttttacgtc gtagctcagg cacaaccatt
     3301 ttcagggctg tggtgtttat gcttacacca tggttctatc gagataatgg tagtccgggc
     3361 ggggcagcct tcgggctggc cggtttccaa taaggccggt tacgccaacc ccgttcgggc
     3421 tgccaccagt gaaattggcg tttccggtgg tagcaataac cgctacttat tggaggctgc
     3481 catcatggct acaatcctca ccccgtcaca cccgcaatat gtctttgtgt ttgccgcaat
     3541 ccgccgtgct gacactcacc cccgcatctg tatgcttcgc actgtctcct gcgatgagcg
     3601 ttccgcgcgc cgtttgctag tccgagatta tgtactctcc ctttctgcac ggctgcctgc
     3661 cggggaggtg acgctatgaa ccagtttgag atttcctacg acgatgttgt aaggctgaaa
     3721 catttacgca atgtgggtga gtatgtaact ggcatggcag tcctgcagga ctgttatgaa
     3781 aagccagcag gtgcccaatg cgaacaactg gtttccctca tttatctgat gacagagcaa
     3841 ctggatggag tggtgcaacg ctgccatgac gacctgatga atgcggaggt ggcctgatga
     3901 tatgctgtga atctcttctg gcacttcgtg ccgctctgta tcgccgtgcc gttgcctgtg
     3961 cctggctggc tctaagcaat catcaggaac gttattccgg cctgacgctc gctgaacttg
     4021 aagatgccat agcccgcgag ctggaagggt tctatctgcg ccagcatggg cagcaaagag
     4081 ggctggaaat tgcctgcgcg ttgctttcgg atctgatgga gtcggggccg cttaaagcct
     4141 gtccggttct ctcactgctc ggaatgacgg tcatggatga actttgttcc cgtcacctca
     4201 acaaaccagc gctgcactaa ggagggccgc acaatgtcag gaatgaaagt tagccaggct
     4261 gagaaagcag ctcggggtca ctggtcaaga attttacctg cgctgggcgt aaatgtactg
     4321 aaaaatcggc accagccctg cccggtctgt gccgggaaag accgctttcg atttgatgac
     4381 caggaagggc ggggaacgtg gttctgtaac cagtgcgggg caggtgatgg cctggcgctt
     4441 gtaagtaaag tactggatgt aggcattagt gaagcggcag acagaataaa cggcattatc
     4501 ggaaacctgc tgccagtatc tcagggaatg cttgaatctg gttctcctga aaaagaggac
     4561 gggagaaaag ctgcagcagt gctggctgcc cgtttgtttg ataagtcccg ccagaccact
     4621 ggcaatgcct atctgacgag taaagggttt cctgcactgc cttgccggga attaaccgct
     4681 atgcataaag tcggtggtgt ggcatttcgc gcgggagatc ttgtcgttcc attgtatgca
     4741 gatggagagc tggtaaatct gcagttaatc aacgctaatg ggggcaaatg cttccttaaa
     4801 ggcggtcagg ttaagaatgc cttttacctg gttgaaggta ctgccaaagc agccaaacgg
     4861 ctctggatag cggaaggata tgccaccgca cttactatca actatctgac tggcgatgct
     4921 gtcatggtgg ccttttcgtc cgtcaatttc ctttccctgg cgagcattgc ctgcagtgag
     4981 tacccaacgc accagataat tattgctgct gaccgcgatc tcaacggtgc ggggcaaaca
     5041 aggggcgcag ctgttaccgg ggcctgcaat tgcacaatgg cgctcccgcc tgtgtttggt
     5101 gactggaacg atgcattcac gcaaaacggc gaagaagcca cccggcatgc aattcatgaa
     5161 gtaataaaac cagctgttgc cagccccttc gacacaatga gcgaagctga atttaccgcg
     5221 ctgagcgtca gcgaaaaagc gcagagggta gtggatcact ataaaaattc actggcagta
     5281 gacccgaacg ggcagctcct ttcacgctat gaggcggggg cctggaaagt tatctattac
     5341 gccgattttg cccgtgatgt cgctgcgctg tttcagcgcc tcgacgcacc tttttcatcc
     5401 gcgaaaattg cgtctctcgt ggaaaccctc aaactgatcg ttccgcaaca gcagaatccg
     5461 gcgcggcaac ttatcggatt tcgcaacggt gtgctcgata cccggacagg attgttcagc
     5521 ccgcacgata agaagcactg gttacgtacg ctgtgcgagg tggattacac gcagcccgtt
     5581 gacggtgagt cactggaaac ccatgccccg gcattctggc gctggctgga tcgtgccgca
     5641 ggttttaatc ctgaaaaacg ggacattatt ctggctgcat tgtttatggt gctggctaac
     5701 cgttatgact ggcagctgtt tctggaggtc actggccctg gcggaagtgg aaagagtatt
     5761 cttgctgaaa tagcaaccat gctggcgggt gaagataacg cgacctcggc aaccattgaa
     5821 atgcttgagt cgccaagaga acgagctgcg ttaataggtt tttcactgat tcgacttccc
     5881 gaccaggaaa agtggagcgg tgacggggcc ggactaaaag ccatcactgg cggcgatgcg
     5941 gtatccgttg atcccaaata tcagaacgcc tattcaaccc acatcccggc ggtcatcctg
     6001 gctgtgaaca ataatccgat gcgcttcact gatcgtagtg gtggagtttc acgccgaagg
     6061 gtgatcctgc atttccccga ccagatagcc ccggaggaac gcgataccca gctcaaagag
     6121 aaaattgcca gtgagctagc ggtgattgtt cgccagctta tgcagcgttt cagcgaccca
     6181 atgagtgcca ggacattgct tcagtcacag cagaactccg atgaagcgct caccatcaaa
     6241 cgtgatgctg attcagcttt tgatttttgc ggctaccttg aggtcctacc tgacaccacg
     6301 ggcatgttta tggggaacgc taatattgtt ccacgtcagc ctcgaactta cctctaccat
     6361 gcctatctgg tctacatgga ggctaacggc tataaaaata cgctcagtct gaccatgttt
     6421 ggcaaggggc taccgttaat gctcaaggaa tatgggctgc agtatgagaa acgacggacc
     6481 aatcaaggaa tgcagactaa tctggcccta agagaggaaa gcaatgctga ctggttgcca
     6541 aaatgcgatg agtttgcagc gaaataactg acctaagacc ggctgtgccg gtcttaaatc
     6601 cgctacagga aattatctca aaatatatat cgataaaatt taaaagttat atgctaaggc
     6661 gtcagcggaa aatgcatcta attcattgtt agatgatgtt tatttttgaa ttgatttaaa
     6721 catcccatcg agttgtaatt tattt
//
LOCUS       DAZFDC010000191         6599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFDC010000191 DAZFDC010000000
VERSION     DAZFDC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.191"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..2999
                     /gene="ehxA"
                     /locus_tag="ACHJ48_004438"
     CDS             3..2999
                     /gene="ehxA"
                     /locus_tag="ACHJ48_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO4709849.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGYD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYGQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            3049..5169
                     /gene="ehxB"
                     /locus_tag="ACHJ48_004439"
                     /gene_synonym="hlyB"
     CDS             3049..5169
                     /gene="ehxB"
                     /locus_tag="ACHJ48_004439"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO4709850.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFIRELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            5173..>6599
                     /gene="ehxD"
                     /locus_tag="ACHJ48_004440"
     CDS             5173..>6599
                     /gene="ehxD"
                     /locus_tag="ACHJ48_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO4709851.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINES"
BASE COUNT         2131 a         1030 c         1486 g         1952 t
ORIGIN      
        1 atatgacagt aaataaaata aagaacattt tcaataatgc gacattgact acaaaatcag
       61 catttaatac agcatcatca agcgtacgtt ccgctggaaa aaaactcata ttattaatac
      121 ctgataatta tgaagctcag ggcgtgggta ttaatgagtt ggtcaaagct gctgatgagc
      181 ttggaataga aatacaccgt actgaacgag atgatacagc gattgcaaac cagttttttg
      241 gtgcagcaga aaaagttgta ggattaactg aacgtggtgt tgcaatattc gcaccacaac
      301 ttgacaaact tctgcagaag tatcagaaag ttgggagtaa aataggagga accgctgaaa
      361 atgtaggtaa taatctggga aaagccggaa cagttctctc agcactacag aattttacgg
      421 ggattgcttt atcaggcatg gctcttgatg aattgctgag aaaacaacgg gaaggagagg
      481 atataagtca gaatgatatt gccaaaagta gtattgaact tattaatcag cttgtagata
      541 cagtatcaag tataaacagt accgttgatt cattttctga gcagcttaac cagcttggct
      601 catttttatc cagtaaacct cgattaagtt ctgttggtgg gaaattacaa aatttaccag
      661 acctgggccc cctgggggat gggctggatg ttgtctccgg aattctttct gctgtatcag
      721 caagctttat tctgggaaac agtgacgcac atacaggaac aaaagctgca gcgggtatcg
      781 aactgacaac tcaggttctt ggaaatgttg gtaaagctgt ttcgcaatat attctggctc
      841 agagaatggc acaggggtta tcgacaacag ctgcaagtgc gggtctgatc acatcggctg
      901 ttatgctggc tatcagtcct ctttctttcc tggctgctgc agataaattt gagcgagcta
      961 agcagcttga atcatattct gaacgattta aaaaattgaa ttatgaaggg gatgctttac
     1021 tcgcagcctt tcataaagaa accggagcta tagatgcagc cctgacaaca ataaatactg
     1081 tcctgagttc tgtatctgcg ggagttagtg cagcctccag tgcatccctc ataggggccc
     1141 cgataagcat gctggtgagt gcattaaccg gtacgatatc tggcattctg gaagcatcaa
     1201 aacaggctat gtttgagcac gttgcagaga aattcgctgc tcggatcaat gaatgggaaa
     1261 aggagcatgg caaaaattat tttgagaatg gatatgacgc aagacatgct gcgtttttag
     1321 aagactctct gtctttgctt gctgattttt ctcgtcagca tgcagtagaa agagcagtcg
     1381 caataaccca gcaacattgg gatgagaaga tcggtgaact tgcaggcata acccgtaatg
     1441 ctgatcgcag tcagagtggt aaggcatata ttaattatct ggaaaatgga gggcttttag
     1501 aggctcaacc gaaggagttt acacaacaag tgtttgatcc tcaaaaaggg accatagacc
     1561 tttcaacagg taatgtatca agtgttttga catttataac accaacattt accccaggag
     1621 aagaagttag agaaagaaaa cagagtggta aatatgaata tatgacatct cttattgtaa
     1681 atggtaagga tacatggtct gtaaaaggca taaaaaatca taaaggtgta tatgattatt
     1741 caaaattgat tcagtttgtt gaaaagaata acaaacacta tcaggcgaga ataatttctg
     1801 agctcggaga taaagacgat gtggtttatt ctggagcagg ctcatcagaa gtatttgctg
     1861 gtgaaggtta tgataccgta tcttataata agacggatgt tggtaaacta acaattgatg
     1921 caacaggagc atcaaaacct ggtgagtata tagtttcaaa aaatatgtat ggtgacgtga
     1981 aggtattgca ggaagtcgtt aaggaacagg aggtgtcagt agggaagcga acagagaaaa
     2041 tacaatatcg tgattttgaa ttcagaaccg gtggaattcc ttatgatgta atagataatc
     2101 ttcattctgt tgaagagctc attggcggaa aacatgatga tgaattcaaa ggcggtaagt
     2161 ttaatgatat attccatggc gcagatggga acgattatat cgaaggtaat tatggtaatg
     2221 atcgactata cggcgatgat ggggatgatt atatatccgg aggacaggga gacgaccagt
     2281 tatttggtgg tagtggaaac gataaattga gtggagggga tggtaataat tatctgacag
     2341 gaggaagcgg taatgatgag cttcaggcac acggagctta taatattctg tcaggtggta
     2401 ctggtgatga taaactttat ggtggtggtg gtattgatct tctggatgga ggggaaggta
     2461 atgactatct gaatggtggt tttggtaatg atatttatgt ttatgggcaa aactatggtc
     2521 atcatacaat tgcagatgaa ggaggtaaag gagatcgttt gcacttatct gatattagct
     2581 ttgatgatat cgcatttaag agagttggaa atgatcttat catgaataaa gccattaatg
     2641 gtgtactttc atttaatgag tcaaatgatg tcaatgggat aacatttaaa aactggtttg
     2701 cgaaagatgc ctcaggagca gataatcatc ttgttgaggt tataacagat aaagatggtc
     2761 gagagataaa agttgataag atacctcata ataataatga acggtcaggt tatataaaag
     2821 ccagtaatat agcatctgaa aaaaacatgg ttaatatcac cagtgttgcc aatgatatta
     2881 ataagattat ttcttcagtt tcagggttcg attcaggtga tgaacgatta gcatctttat
     2941 ataatttatc cttacatcaa aacaacacac actcaacaac tttaacgaca actgtctgag
     3001 actaaaagag gaggtgatgg ccattccagg aggaatggtc atcgttatat gatgagtaaa
     3061 tgtagttctc ataatagtct gtatgcactg atattgcttg cacaatatca taatataact
     3121 gtcaatgctg aaactataag gcatcagtat aatacccaca cacaagattt tggggtgact
     3181 gaatggttac tggcagcgaa atctattggc ttaaaagcaa aatatgtaga aaaacatttt
     3241 tccagattgt caataatttc tttacctgcg ttgatatggc gggatgacgg taagcattat
     3301 atattgtctc gtattactaa agattcatca cgctatcttg tttatgatcc agaacaacat
     3361 cagtcactaa cttttagtcg ggatgagttt gaaaaactgt atcagggaaa agtcattctg
     3421 gttacgtcaa gagcaacagt agtcggagag ttagctaaat ttgatttttc ttggtttatc
     3481 ccctctgttg tgaaatacag gaggatttta cttgaggtgt taactgtttc tgcttttatt
     3541 cagtttcttg cgttaataac acctcttttt tttcaggttg taatggataa ggttttagtt
     3601 caccgggggt tttcaacgtt aaatattatc acaatagcat ttattatagt gatacttttt
     3661 gaagtgatat taaccggagc cagaacttat attttctctc atactacaag tcgtattgac
     3721 gtcgaactgg gtgctaagtt attcagacat ttgcttgcat tgcctgtttc atattttgaa
     3781 aatcgcaggg tcggagagac cgttgccaga gtaagggaac tggagcaaat tcgtaatttt
     3841 ttaaccggac aagcattgac atcagttctt gatctatttt tttctgtaat atttttttgt
     3901 gtcatgtggt attacagccc tcaattaaca ctggttatat tattgtcact accttgttat
     3961 gttatatggt cattgtttat atcaccctta ttacgtcgac gtcttgatga taagtttctc
     4021 aggaatgcag aaaatcaagc ttttcttgtc gaaacggtaa cagcaataaa tacaatcaaa
     4081 tccatggcag tatcaccaca gatgattgct acatgggata aacaactggc cgggtatgtt
     4141 gcttccagtt tcagggtaaa tctggttgca atgacagggc agcaggggat acagctgata
     4201 cagaaaagtg taatggtaat tagtctatgg atgggagcac atcttgtcat atcgggagag
     4261 ataagcatcg ggcagttaat cgcgtttaat atgcttgcag gtcaggtcat tgctcctgtt
     4321 atcagactgg ctcatctttg gcaggatttt cagcaggttg gtatttctgt tgagcgcctt
     4381 ggtgatgtat taaatacacc ggtagagaaa aagtcaggca gaaatatact gccggaaatt
     4441 cagggggata tcgaatttaa aaatgtcaga tttcggtatt cttctgacgg taatgttatt
     4501 ttgaataata ttaatttata catatcaaag ggggatgtta tcggtatagt tggccgttct
     4561 ggttcaggaa aaagtacatt aacaaaactg cttcagcgct tttatatacc agagaccgga
     4621 cagattttaa ttgatgggca tgatttatca cttgcagatc cagaatggtt acgacgccag
     4681 attggtgttg tattgcagga aaatatacta ttaaatcgta gtattatcga taatattaca
     4741 ttagcttctc cggctgtatc tatggaacag gctattgagg cagccagact tgcaggtgcc
     4801 catgatttta ttagagaact aaaagaaggg tacaatacta ttgttggaga acagggcgtt
     4861 ggtctttcag gagggcagcg ccaacggatc gcaatagccc gggctcttgt tacaaatcct
     4921 cgaattctta tttttgatga agcaaccagt gctcttgatt atgagtcgga aaatataata
     4981 atgaaaaata tgtcaagaat atgtaagaac agaaccgtaa ttattattgc acacaggttg
     5041 tcaactgtaa aaaatgcaaa cagaataatt gttatggata atggctttat ttctgaagat
     5101 ggcacacata aagagcttat ctccaaaaaa gacagtttat atgcatattt atatcagttg
     5161 caggcataac gtatgcgctt ttacatgaaa ggtttatggg atttggtttg ccgttataag
     5221 acggtttttt ctgatgtctg gaaaattcgt cacacacttg atgctcctgt gagagaaaaa
     5281 gatgagtatg catttcttcc tgctcacctt gaactcattg aaacaccagt atccagacgt
     5341 tctcattttg ttgtatggag tattttatta tttgtaatta tatctcttct cttatctgtt
     5401 cttgggaaag ttgaagtggt ttcagtagca aatggtaagt ttactcatag tggaagaagc
     5461 aaagaaataa aaccgataga gaatgcgatt gtagaaaaaa taatggtaaa agatggctct
     5521 tttgtaaaaa aaaatgatcc attagttgaa ttgacggtgc ctggtgttga atctgatatt
     5581 ttaaaatcag aagcatcttt gttgtatgaa aaaacagaac aatatcgcta cgcaattctt
     5641 tctgaatcaa tacaacggaa tgagcttcct gaaataagga taacagattt tcctggcgga
     5701 gaagataatg cgggaggtga acattttcag agggttagct cattaataaa agagcaattc
     5761 atgacatggc agaacagaaa aaatcaaaag cagctcacat taaataaaaa aatagttgaa
     5821 cgggatgcag cgcttgctcg tgttagcctg tatgagcatc aggtatcaca ggaaggaaga
     5881 aaactcaatg attttaagta tttgttgaat aaaaaagctg tttctcaaca ttcagttatg
     5941 gagcaggaaa atagctatat tcaggcaaaa aatgaacatg cagtctggct tgcacaggtt
     6001 tctcaacttg aaaaagaaat agaacttgtg cgggaagaac tggcactgga gacgaatatc
     6061 ttcagaagtg aaattatcga gaagcacaga aaatcaacag ataacattgt gttgctggag
     6121 catgagcttg aaaaaaacag gcagagaaaa gcatcatctt ttattaaagc tcctgtgagt
     6181 ggtactgttc aggagttaaa tatacataca gaaggtggtg ttgtcacaac agcagaaacg
     6241 ctgatgatta ttgtccctga taatgatatt ctcgaagtaa cagcatctgt acttaacaag
     6301 gatatcggtt ttatacaacc tggacaggag gtcgttatta aagtagatgc atatccatat
     6361 acacgtcatg gttaccttac agggaaagta aaaaatataa ctgcagattc tgtttctgtt
     6421 ccggatacag ggcttgtatt taacgtgatt atatctgttg atcggaatga tatacaggga
     6481 gaaagaaaaa aaatccctgt tacagctgga atgactgtta tggcagaaat aaaaaccggg
     6541 gtgcgtagtg ttatcagcta tcttcttagt ccattaaagg aaactattaa tgaaagttt
//
LOCUS       DAZFDC010000192         6425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFDC010000192 DAZFDC010000000
VERSION     DAZFDC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.192"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(150..2183)
                     /gene="betT"
                     /locus_tag="ACHJ48_004441"
     CDS             complement(150..2183)
                     /gene="betT"
                     /locus_tag="ACHJ48_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO4709852.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2312..2899
                     /gene="betI"
                     /locus_tag="ACHJ48_004442"
     CDS             2312..2899
                     /gene="betI"
                     /locus_tag="ACHJ48_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO4709853.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2913..4385
                     /gene="betB"
                     /locus_tag="ACHJ48_004443"
     CDS             2913..4385
                     /gene="betB"
                     /locus_tag="ACHJ48_004443"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO4709854.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCNAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4399..6087
                     /gene="betA"
                     /locus_tag="ACHJ48_004444"
     CDS             4399..6087
                     /gene="betA"
                     /locus_tag="ACHJ48_004444"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO4709855.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAM
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDCKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKEALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1578 a         1779 c         1764 g         1304 t
ORIGIN      
        1 taattaataa aatataaaat catgaagtga tacaagagga atttcaggat atgacatgac
       61 aatcatatat aatatatgca taatttttta cagcgagtca atgattcccg cgccatatgc
      121 agacatggcg cggttttatg caataacaat cacgcgtccg ggaacatcac gctatggccc
      181 ggcgcttcac gatggagatg aataaagtta aggtgccgct cgtactggtc aagaatatcg
      241 gtgatcacct gctctttgct gtagtccatc aggtcgttgc cctggctgcc ttctaacagg
      301 aaggtttcca gccggtagta ggtcgatttc ccgctacgtg cgcggtaggt aaagcccggc
      361 accgagtatt gctgcggcca aatctgatag acaaagtttt gctcttcgcc catatgcacc
      421 aacaaatcca gatgccccaa ctgttgtccc tcttccggcg gcaggctttt tagctccacg
      481 tacgcgccgc gcaaccgcaa ctcctgcgcc acttcttcca ttgccgggta acagaccgtc
      541 tccatcatct gtttagtgta acgcgtgccc ggataattca tcaggcgcga gagacgtttt
      601 ttccagctca ggcgatcctg aagccccagc ggtcgcggtg cggtatcgcg gttggcactt
      661 tcacggcggt aatcttctac cttcagagat ttatacaacc ccgccatcac gaagaagatc
      721 acaaagctga acggcagccc cataatcacc gtggtgtttt gcagcgcgga tatcccgttg
      781 gtcatcagca tgccgagcgt cagcaggcca atcgccaccg accagaagac gcgcagccag
      841 ccgggggcgt cgctgttgat atctttaagc tgcgaggtga aattccccag caccagcgcc
      901 ccggagtccg ccgaggtcac ataaaacagc aggccagtaa tggtggcgac ggaggcgcta
      961 aaggtaaacg ccggatactg cgccagcagg ctgtagaagc cgcgctccgg atggaccatc
     1021 gcttcctcgg caaatgccgc gccgccgtgg atgatttcat acagcgcgct attgccgaac
     1081 accgagagcc ataacagcgt gaaggtaaac ggaataatca acgtgcccag cacgaactgg
     1141 cgaatggtac gcccacgcga gatacgcgcc aggaacaagc cgacaaacgg cgaccatgcc
     1201 acccaccatg cccagaagaa gagcgtccag ttattcatcc actcaaccgg gcggtcgaag
     1261 gcaaaactgt tgagcgtcat gcccataaag cgattcacat agtcgccaac attcagcacc
     1321 agtgcattaa gcaggaacga agtgtcgccc ataaacaaca cgaacaggat caatcccagc
     1381 gccagcgcga cattaagctc cgataacacg cgaatgccct tatcgacacc ggatgtcacc
     1441 gagatagtgg cgattatcac cgacaaggcg atcagtgccg cttttgccgc catcgaatcg
     1501 ggaatatcaa acagtacgct caagccatag ttaagctgca ccacaccgat accgagcgta
     1561 gtggcaatac cgaagatagt gccgatcacc gctgcaatat ccactgagtg acctatcggc
     1621 ccgttaatcc gtttaccgaa gatcgggtac agcgccgagc ggatggtgag cggcaaatta
     1681 taacgatagc taaagtatcc gagcgccatg cccatcagcg catacatcga ccagccggtt
     1741 aagccgtagt gaaacagcgt ccagaccatc gcctgacgcg cggcctcaat cgtctgtccc
     1801 gcgccttccg gcggctgcat atactgcgtt accggttcgg ctacggaaaa gaacatcaga
     1861 tcgataccga tcccggcagc aaacagcatc gccgcccaac tcagcaggct gaattccggt
     1921 ttggattgtt ctggcccgag cttcaccgaa ccaaaacgcg aacaagcgat acagaccaca
     1981 aagacaatat agagcgttgc cgccagcaga tagtaccaac cgaaggtttt agaaacccag
     2041 tccagcgtgc ggccaatcca cagggccgag aagtcgcgaa acaggatcgt tgtcagggaa
     2101 aacaacaaaa tcagtccggc ggaggtgtaa aacaccaccg gattgatttt gtccttttcc
     2161 ctgctgtgtg aaaggtctgt catccagtat ccccactgtt attgttacca tttaaaatca
     2221 aattcgtaac aattaagaca cattttatat tgaacgtcca atcaataacc gctttaatag
     2281 ataaacaacg ctgatgaatg gagtggcaaa aatgcccaaa ttggggatgc agtcgatccg
     2341 gcgcagacaa ctgatcgacg ccacactgga agcaataaat gaagtgggca tgcacgatgc
     2401 aacgatcgcg cagatcgccc gccgtgcagg cgtttctacg gggatcatca gccactattt
     2461 cagggacaaa aatggtctgc tggaagcaac catgcgcgat atcaccagtc agctgcgtga
     2521 cgcggttttg aatcgattac atgcacttcc gcagggcagt gcagagcagc gattacaggc
     2581 gattgttggc ggaaacttcg atgaaacgca ggtgagcagt gcggcgatga aagcctggct
     2641 ggcgttctgg gccagcagta tgcatcagcc gatgctctat cgtttacagc aggtcagcag
     2701 ccgccgcttg ctgtcgaatc tggtgagcga gtttcgccgc gaattgccgc gccaccaggc
     2761 acaggaagcg ggctacggcc ttgccgcgct gattgacgga ttatggctgc gcgcggcact
     2821 gagcggcaaa ccgctggata aacctctcgc acactcgctg acccgtcact ttattactca
     2881 gcatttaccc accgattaac cgaggagacg tgatgtcccg aatggcagaa cagcagcttt
     2941 atatacatgg tggttatacc tccgccacca gcggtcgcac cttcgagacc attaacccgg
     3001 ccaacggtaa cgtgctggcg accgtgcagg ccgccgggcg cgaggatgtc gatcgcgccg
     3061 tgaagagtgc ccaacagggg caaaaaatct gggcggcgat gaccgctatg gaacgctcgc
     3121 gtattctgcg tcgggccgtc gatattctgc gcgagcgcaa tgatgaactc gccaaactgg
     3181 aaaccctcga caccggaaaa gcgtattcgg aaacctcaac cgtcgatatc gttaccggtg
     3241 cggacgtgct ggagtactac gccgggctga tcccgtcgct ggaaggcagc cagatcccgt
     3301 tgcgtgagac atcatttgtc tacactcgcc gcgaaccgct gggcgtggtg gcagggattg
     3361 gcgcatggaa ctacccgatc cagattgccc tgtggaaatc cgccccggcg ctggcggcgg
     3421 gtaatgcgat gattttcaaa ccgagcgaag tcactccgct taccgcgtta aagctggctg
     3481 aaatttacag cgaagcgggc ctgccggacg gtgtatttaa cgtgttgccg ggcgtgggcg
     3541 cggagaccgg gcaatatctg accgagcatc cgggcattgc caaagtgtcg tttaccggcg
     3601 gcgtcgccag cggcaaaaaa gtgatggcta actcggcagc ctcttccctg aaagaagtga
     3661 ccatggaact gggcggtaaa tcaccgctga tcgttttcga cgatgcggat ctcgatctcg
     3721 ccgccgatat cgccatgatg gcaaacttct tcagctccgg tcaggtgtgt accaatggca
     3781 cccgcgtctt cgttccggcg aaatgcaacg ccgcatttga gcagaaaatt ctggcgcgcg
     3841 ttgagcgcat tcgcgcgggc gacgttttcg atccgcaaac taacttcggc ccgctggtca
     3901 gcttcccgca tcgcgataac gtgctgcgct atatcgccaa aggcaaagag gaaggcgcgc
     3961 gcgtactgtg cggcggcgat gtactgaaag gcgatggcct cgataacggc gcatgggttg
     4021 caccgaccgt gttcaccgat tgcagcgacg agatgaccat cgtgcgtgaa gagatcttcg
     4081 ggccggtgat gtccattctg acctacgagt cggaagacga agtcattcgc cgcgccaatg
     4141 ataccgacta cggcctggcg gcgggtatcg tgacggcgga cctgaaccgc gcgcatcgcg
     4201 tcattcatca gctggaagcg ggtatttgct ggatcaacac ctggggcgaa tccccggcag
     4261 agatgcccgt tggcggctac aaacactccg gtattggccg cgagaacggc gtgatgacgc
     4321 tccagagtta cacccaggtg aagtccatcc aggttgagat ggctaaattc cagtccatat
     4381 tctaaccagg aggtttattt gcaatttgac tacatcatta ttggtgccgg ctcagccggc
     4441 aacgttctcg ctacccgtct gactgaagat ccgaatacct ccgtgttgct gcttgaagcg
     4501 ggcggcccgg actatcgctt tgacttccgc acccagatgc ccgctgccct ggcattcccg
     4561 ctacagggta aacgctacaa ctgggcttac gagacggaac ctgaaccgtt tatgaataac
     4621 cgtcgcatgg agtgcggacg cggtaaaggc ctgggtggat cgtcgctgat caacggcatg
     4681 tgctatatcc gtggcaacgc gatggatctc gacaactggg caaaagaacc cggtctggag
     4741 aactggagtt atctcgattg cctgccctac taccgcaagg ccgagacccg cgacgtgggc
     4801 gagaacgact accacggcgg cgacggcccg gtgagcgtca ccacctccaa acccggcgtc
     4861 aatccgctgt ttgaagcgat gattgaagcg ggcgtgcagg cgggctaccc gcgcacggac
     4921 gatctcaacg gttatcagca ggaaggtttc ggcccgatgg atcgcaccgt cacgccgcag
     4981 ggccgccgcg ccagcaccgc gcgcggttat ctcgatcagg ccaaatcgcg cccaaaccta
     5041 accattcgta ctcacgccat gaccgatcac attatttttg actgtaaacg cgcggtgggc
     5101 gtcgagtggc tggaaggcga cagcaccatt ccgacccgcg cgacggcgaa caaagaagtg
     5161 ctgttatgtg caggcgcgat tgcctcaccg cagatcctgc aacgctctgg cgtcggcaac
     5221 gctgaactgt tggccgagtt tgatattccg ctggtgcatg atttacccgg cgtcggtgaa
     5281 aatcttcagg atcatctgga gatgtatctg caatatgagt gcaaagaacc ggtttccctc
     5341 taccctgccc tgcagtggtg gaatcagccg aaaatcggtg cggagtggct gtttggcggc
     5401 accggcgttg gtgccagcaa ccactttgaa gcaggcggat ttattcgcag ccgagaggaa
     5461 tttgcgtggc cgaatattca gtatcacttc ctgccggtag cgattaacta taacggctcg
     5521 aatgcagtga aagagcacgg cttccagtgc cacgtcggct cgatgcgctc gccaagccgt
     5581 gggcatgtgc ggattaaatc ccgcgacccg caccagcatc cggcaattct gtttaactac
     5641 atgtcgcacg aacaggactg gcaggaattc cgcgacgcaa ttcgcatcac ccgcgagatc
     5701 atgcatcaac ccgcgctgga tcagtatcgt ggccgcgaaa tcagccccgg cacggaatgt
     5761 cagacggatg agcagctcga tgagtttgtg cgtaaccacg ccgaaaccgc cttccatccg
     5821 tgcggtacct gcaaaatggg ctacgacgag atgtccgtgg ttgacggcga aggccgcgtg
     5881 catgggctgg aaggcctgcg cgtggtggat gcgtcaatta tgccgcagat tatcaccggg
     5941 aatttgaacg ccacgacgat tatgattggc gagaaaatgg cggatatgat tcgcgggaag
     6001 gaagcgttgc cgaggagcac ggcgggatat tttgtggcaa atgggatgcc agtaagagcg
     6061 aaaaaaatga gtcgtgattt gaactgactc aggaaccgat cagattgaga gttaccgttc
     6121 caggtatggg gacagaatcg ttatataaaa cataggggta actctcatgc ttcatagtga
     6181 caacctcgcc agcaaattct aagctgactc gttcaatctc gctgatgaag gtactatgaa
     6241 tgcactgata aaccgcaggt gaaatgccac tcagcttaag aattacgccg ctagtgttcc
     6301 tggctatggt gacttgttca ctcccgctca cggttagcat atacgaaaag ggttcttgta
     6361 acgaggacaa aaataagtca gaccaaaaca acgtggagct ttaaacacaa ttcgtataat
     6421 ttatt
//
LOCUS       DAZFDC010000193         6347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFDC010000193 DAZFDC010000000
VERSION     DAZFDC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.193"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(32..346)
                     /locus_tag="ACHJ48_004445"
     CDS             complement(32..346)
                     /locus_tag="ACHJ48_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO4709856.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQANQPSGVECNFYKIKKNIQTLF
                     PE"
     gene            complement(351..1310)
                     /locus_tag="ACHJ48_004446"
     CDS             complement(351..1310)
                     /locus_tag="ACHJ48_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO4709857.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVATAIREACGVNATFIADGVPQFALVNGLYAMNKE"
     gene            complement(1387..4209)
                     /locus_tag="ACHJ48_004447"
     CDS             complement(1387..4209)
                     /locus_tag="ACHJ48_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO4709858.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAYRVENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRQKGKKSWQAERYARGNLRKIFERLERTDHRWLTQGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMTESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFTTYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPGVEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRRDI"
     gene            complement(4216..4581)
                     /locus_tag="ACHJ48_004448"
     CDS             complement(4216..4581)
                     /locus_tag="ACHJ48_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709859.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATACRDTLNGMTK"
     gene            complement(4654..4884)
                     /locus_tag="ACHJ48_004449"
     CDS             complement(4654..4884)
                     /locus_tag="ACHJ48_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO4709860.1"
                     /translation="MADRKQHRAIAERRHIQTEINRRLSRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLHEFQQLIQQQNKLH"
     gene            complement(5207..5506)
                     /locus_tag="ACHJ48_004450"
     CDS             complement(5207..5506)
                     /locus_tag="ACHJ48_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709861.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDQL
                     EHARTTAPAIRLTLHHEIADFCATLGAPGEPETPEAMQQELLQRIDNVFDFFLNQ"
     gene            complement(5503..5769)
                     /locus_tag="ACHJ48_004451"
     CDS             complement(5503..5769)
                     /locus_tag="ACHJ48_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO4709862.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            complement(5766..5969)
                     /locus_tag="ACHJ48_004452"
     CDS             complement(5766..5969)
                     /locus_tag="ACHJ48_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709863.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            complement(5993..6346)
                     /locus_tag="ACHJ48_004453"
     CDS             complement(5993..6346)
                     /locus_tag="ACHJ48_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709864.1"
                     /translation="MSRAAQKDNQHMAALYALTIAIATPEAAKVIEEQSKEIDTLKTQ
                     STVAAMNPSSIGRCIYILGSAMMLQYTIIAELHGKYLITPYHTKESELLTNLRLIERS
                     QAVFIDDAQRAVFNA"
BASE COUNT         1225 a         1652 c         1815 g         1655 t
ORIGIN      
        1 gtattttatt agttcacata caattatcag attattctgg gaataaggtc tgaatattct
       61 tttttatttt ataaaaattg cattcaactc cgctaggctg attagcctgg tatgtttcct
      121 tgatgctaac ggtattgaag gggaggttgt cctctggcca ttcggccatc cagtaggcaa
      181 atgctgggtt gcgctttatg agtgctagcc cagccagaaa ggccgcgcgt tgcgcgcggc
      241 tgcgttcgga ggctggcagg ctatcgaggt aattgcacgc ctcctgttca ctcttgacgg
      301 cggcgggctt cagatagaaa cttatccgtc tggttggtgt cgtcattggt ttactccttg
      361 ttcattgcgt acaacccatt aaccagagca aactgtggca ccccgtccgc gatgaaagtc
      421 gcattaactc cgcaggcttc gcggatagcg gttgccacaa tctccgcccc gccaccgaca
      481 accatcaccc gcccgtaacc cgaaaaaacc gccagcgcgc ggatcacgcg ttgtttcagt
      541 gtttcttcct tttcacgaat aaccgccatc aggctggcgt aatgcgcgtc attgtggatg
      601 tgctggcgca gccaggcttc atcatggcga tgttcgataa tggtattggc gatgtggtga
      661 ctggtgcgca taccgttagt ggccatcacc gacagtacgg catcggccat cagagaaacg
      721 cctacgtgtg gatcgcaaaa cacctggctg atacctgcca gttgcccctg aacctttgcc
      781 acatccagcg tggttccgcc caaatccaca atcagcaggg attcaaacgg actcatgtca
      841 gccagtgcct taaagccagc aggaatggat tcaggcataa cccgtacgtt acggatagtg
      901 aatgcttcgc cgttctggta ctccaccggg cgcatgacgt tcgctttttt gcggttgatg
      961 ttggccatgt ccggctgtgc gtttgtatcg aaatactcgc tcagtggcag ggtgacaacc
     1021 acatccactt cctgtggtgt gatgcctgat ttgaccagcg cgtgatgaat ggcaattaca
     1081 ttcacatcgc tgtactggta ttgcgtgtcg gtcgtctgga caaagcgatc gctgaccgga
     1141 tcaaaaccat agcgcacgcc atcaagcatg tagttcgcgg gctgcgtgcc accgaatggc
     1201 gcagaccatt ccgacttgaa gctgttcggg ctgatggcgt tgcggcgttc gccgttctca
     1261 gtccatgcca gcttgatgtt ggtggagccg tcgtcgatac aaattttcat gtcgcttttc
     1321 cttatgttga ttaattaatc ggttacagga tttttaaatc ccgcttttgc ctgttttgtg
     1381 cgcgcttcat atatcgcggc gcgttttttg ctcatttacg ggatttgtga gtcccgtttc
     1441 tgtctgtttt ttgtttccac tggtcaggcc accccgcagc aggtctgctt tgcggctggc
     1501 gcgttcagtg gtttcactga ttctctgtgc gtgctctgcg tcgcggatgg cgcgcagcat
     1561 gtcagaaagc acggtaacgg gtgttttcat ggtgttctgg tcctgctgaa gtgtggatgc
     1621 caggcgtgcg gcggcttcag ggtctgatgc ccccagctgt gccagatagc tggcgaccgg
     1681 gttatggcgg atctccgtgc tgcttacgcc atgattacgg ctcaggcgct gccagagctg
     1741 cgtgattcgg ctgtccgggc gggtatccgg tttgcgtaca atttcaaatc cctgcggtgc
     1801 aatgatgctg ccgtcaacgt acagactgcc gccccgtaac aggtgctgca tctgttgttc
     1861 accgatatgc aggccgagag attcagcaga ctcccgccat tctttagcga gtaattcgtg
     1921 gttatcaggc aaaggccgct gctgtttgcg gctctgtgtc cagctctgca tttcatcact
     1981 gctgtttttt gcctgtttgt cacgaagcga acgcatcagc gcccggcgtt cgtgccgttt
     2041 cagtgagcgc atccattcgt tcacgtcaac gccgtcagga agctgcggcc acggtgctgg
     2101 ccgttcttcc ggctgttctg tcccgttgtt gtccgtttcc tgtacacggg gacagttatt
     2161 gccacgagtc caaggggcgg cagggccgcc ctgaaggtca aaaccatttt cgcgggcgct
     2221 gtcttctact cccggtttac gtcttaccag cttccagtta tccgggtgcg tgcacacacg
     2281 ggaggattcc ccgatgagtg gtgaccagat cccgtaaatc tgtacgctct gttcgccgta
     2341 atcgttcagc tcatctgcga ggtcgtaggc ggtgcgaatc aggtagtcct tgcgtggaac
     2401 aagtacgcct ccctgtttct caatgtaggt ggtaaaacat ccggcatcag cggcagcaag
     2461 aaccgcatcc attgcgtcat ccttcagccg ttgcgggcct tccgggttgc gtgccatctg
     2521 gctggcaagg cgacgcagtt cacgccacac ctgacgggag gggatgccaa agaactggaa
     2581 ctggcggacc cggtgaaggc gcgcccagcc aatggcgcgt tccacgctct cggtcattga
     2641 ttttccggtt tcgtggtcaa cgcgtggttt gcctgttttc gggtcgatgc catccacggc
     2701 gcggctgtcc aggttttttc cgatgtaggt ggcgatgtag ctggttggcg tgccttttga
     2761 gccgtcgaca tactccgcct taaagcgcgg agtaatatca tcgcccagct cgtgacgatc
     2821 ttcctgaatg gcaatatcgc gggtgtggga cacaatggtg tcgatttctt ccggatgagc
     2881 aaagaccatc atatgccagt gcacggtgcc gtcatggtga ggctccaccg tgcggatgcc
     2941 ataccagcgc aggccgtcgc ggttcagttt cttgcggacc gccgcaaaaa acgtgttaac
     3001 caggtaatcg ctggagtcgc gcatggtggc cccgttccat ttgggattcg gatgaccgtt
     3061 ctctgttgta gcgtggtatt ttgacgggca ggtgacagtc agaaacaccg ctttgtcgcc
     3121 acgggcttcg gccagaagtt ccagcccctt catggtggcc atcatttctg ccttacggtg
     3181 aaccgggtta cttactcccg cgtaatacac tgtctcgaga tcaatcgtga acccgtcttc
     3241 gttttccagc atgaaacttt tcaggaaatc gcgtgttttc tcgcgctgtg cgcgaaactc
     3301 gcttaacgcg tcctggctca gatagggcga tgtttttctg gaaaccagac aggcggcgcg
     3361 gagttgttct tctctccact cgcaacgtaa cagccacagt ttgcgtttcc accattccgc
     3421 acaggtcagg cgaaggattg cgcccggcag cagctccgtg tccggttcgt tcctccggtc
     3481 tttgtctgtt gtcagtgcgt cataatgtgg aggcatggcg tgcaggtgta acgccatgcg
     3541 ggccagcatc tgatacgcct tcagcgttac atccatggtc agttcgccat cggtcgcgcc
     3601 aaagccatcg cagagttttt cgaaggtgct gctgaacatc gccgccgtca tggtggccag
     3661 cgtctgtatc tggtgtttgt tgagctgcgg caggtaaagc aaatcgtcca ggcgttcgcg
     3721 tccggcaagg gagcgataac cctgtgtcag ccagcggtga tcggtgcgct ccagacgttc
     3781 gaatattttg cgcaggtttc cgcgcgcgta gcgttcagcc tgccagctct ttttgccttt
     3841 ctgacgatcg gcttcctgtt ttttgcgcag gaaggagagg tggcgaataa gcggatcgcg
     3901 cagataggac ggcagcaggc gcagcgaggc catggcttca tccaccgcac cgcgtgcctg
     3961 ttttctggcg tctcctgcca gtgtgatggt tttgtcctgt ttttcctgtg cgtccaggct
     4021 tttattaatc aggttgccca gcggcgttgc ggagaacgcc gcatcagcca tttcctggcg
     4081 gcgctcgttc tctacccggt aggcatccag ccaggaggaa agcgcggatt caggagcggg
     4141 gatccccgtt ccttcacgcc ccactgcgtg gcgcggttgt tgccagtccc tgatgtactc
     4201 tgccgtcata gtgatttact tcgtcatgcc attcagggtg tcgcggcagg ctgtagccag
     4261 ccgctgaatt tccagcacgg tgtcttctgt gtcggcatgg cgatgtgtga tgcggatgct
     4321 gtcggcaatc acatcgacga ttgcagagga tgggcgctgg taaatgccaa taacggacgg
     4381 ggtgccacct tcaatgcggt aaagcctgta atttccctcg tggctgtcaa tcatgtagcg
     4441 accatcaata acaatctttc cgtcagcgag ctgcggtaca ggcagggatt tcaggtacat
     4501 gtcataacga tcacgcacgc gagcggcaag atcacgttct gtgttgagca ggtattcaag
     4561 aaagtcgttg gcgagaatca ttgcggcaat cctcttgtta cagatgtgcg aaggcctccc
     4621 gccgcaaggt gcaggaaagg cccggaacag gaattaatgg agtttgtttt gctgctggat
     4681 gagctgttga aactcgtgca gatcatccgc cagatagctg aaaacagagg cggaataaat
     4741 gtttgatagt gcgtggctgc gctcatgcag catattgatg tgcatgattt gcgcgacgcg
     4801 tgatgcgcgg gaaagtctgc ggttgatttc agtctggatg tgacgacgct ccgcgatagc
     4861 gcggtgctgt ttgcggtctg ccatggtgtg gcctctttgc tcggtgatag aaataactca
     4921 ccatccagag ttgagaatct cggggtggcg agacgtacag ggttctcaac accggagagc
     4981 aaagaatccg gcccgaccga agtcggcccc gtacgccccg ccataattct gacgcgaaaa
     5041 agacgtggca atacagtacg cacaaaaaaa ccgctagcgc ggttatgcgc tttgctctgt
     5101 atcgggttga gaatcccggc acccgtttta tgaggtgcag cggaaatgta acctgaccga
     5161 ttgcggcatg gcaagcggtt tttttgtgtg tgcatgttct ggtttcttac tggttcagaa
     5221 aaaaatcaaa aacgttatcg atgcgttgca gcagctcttg ctgcattgct tccggcgttt
     5281 ccggttcgcc aggtgccccc agcgttgcgc agaaatcagc gatttcatga tggagcgtga
     5341 ggcgaatggc aggagccgtg gttctggcgt gctccagctg atccagcagt gccagcacag
     5401 cagacggcga gagcattgcg cgaaatgcca gtaatttttg aggcgttgcc attcgttgca
     5461 gggcaaatgc cagttcgcgt agcttctggt ggttgatggt gctcatgctc tggcttcctt
     5521 cagtagctgg ttaaacatgt gagtaagtgg attgctacac ccgaacggca tcgggtttac
     5581 gtggtaagaa gcctggcctc ctgttttgcg agcgcgacca cctgtgctgc ggtttgttct
     5641 gatgactaag ccgccgcgcc aaagtcggcg taactcagca ttaatggctg tggttggggt
     5701 attcagtgct gcggcgatct ctccgccgct acaccccgga tgagtagcga tgtagtccag
     5761 aatggtcatc tgcgtggctc ctgtacctgt cggataagat ttactcgcgc cacgttggtg
     5821 gcgcagaagt aagtgccgtc agtgaggtag atgtggtgtg catccttttc cgaacgatgt
     5881 ttgtcgatag tggtaatcag gcgttcgtcg acctcgtatt cgcgccctct ggaggtaaag
     5941 cgaacgacgg gaaaatgctt aattgccatt gcgccccctt tgtccagtaa ccctatgcgt
     6001 taaatacggc acgttgcgcg tcatcaatga atacagcttg agagcgttct atcaggcgga
     6061 gatttgtcag aagctctgac tcttttgtgt ggtaaggcgt tatcaggtat ttgccgtgca
     6121 gttcggcaat aatggtgtat tgcagcatca tagctgaacc aagaatataa atgcagcgtc
     6181 caatgctgga cggattcatg gctgcaactg ttgactgtgt tttaagagtg tcgatttctt
     6241 tgctctgttc ctcaataact ttggctgctt caggggtggc tattgcgatg gtcagtgcgt
     6301 aaagcgctgc catatgttga ttatctttct gggctgccct ggacagc
//
LOCUS       DAZFDC010000194         6345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFDC010000194 DAZFDC010000000
VERSION     DAZFDC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.194"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..126)
                     /locus_tag="ACHJ48_004454"
     CDS             complement(<1..126)
                     /locus_tag="ACHJ48_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709865.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTE"
     gene            455..1327
                     /locus_tag="ACHJ48_004455"
     CDS             455..1327
                     /locus_tag="ACHJ48_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO4709866.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVWGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1699..4548
                     /gene="cah"
                     /locus_tag="ACHJ48_004456"
     CDS             1699..4548
                     /gene="cah"
                     /locus_tag="ACHJ48_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO4709867.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            4659..>6345
                     /locus_tag="ACHJ48_004457"
     CDS             4659..>6345
                     /locus_tag="ACHJ48_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO4709868.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         1598 a         1603 c         1785 g         1359 t
ORIGIN      
        1 ctcagtatta tcatagccga aaacttctcc cggacacggg cgttcacgat aatagtcatc
       61 gtcatagttt tcatcgtcag ataatgatat gtcgtattct gaatatgatt caccacaatt
      121 atacatgaaa tcatctcctt tggggggggc aataattagt gagtttattc gaaaaaatga
      181 tgagcgccag accaatccac ccaacttttg attataatca tgaagatact tgtctcagta
      241 caaattgaat caaactcaat gcagcatgat tcacctttat attctctcag ggaaaccaaa
      301 taccactact caactattaa gaacaaaatt cgggatgtat gattatcaga agatacctgc
      361 atcctaaata atttcagaca gatattatcg ttgtgaatcc cccgccacct ttccggtgcg
      421 cggggttttg tcttttttca ccgggaatac atgtatgaat ccgtctgatg ccattgaggc
      481 aattgaaaaa ccgctctcct ccctgcctta ctcgctttcc cgtcacatcc tggaacatct
      541 gcgcaaactc accagtcacg aacccgtgat tggcattatg ggtaaaagcg gggccggtaa
      601 atcctcactc tgtaatgcac tgtttcaggg ggaggtcacc ccggtcagtg atgttcacgc
      661 cggcacccgg gaagtgcggc gcttccgtct gagtggccat ggtcacagca tggttatcac
      721 tgacctgccc ggggtgggcg agagccggga cagggatgca gagtatgaag ccctgtaccg
      781 tgacattctg cctgaactgg acttggtact gtggctgatt aaagccgatg accgtgccct
      841 gtctgtggat gagtatttct ggcgacacat actgcaccgg ggacatcagc aggtgctgtt
      901 tgtggtgacg caggccgaca aaacagagcc ctgccatgaa tgggatatgg caggtattca
      961 gccttctccc gcacaggaac agaacattcg cgaaaaaaca gatgcggtat tccggctgtt
     1021 ccggcccgta catccggttg ttgccgtatc ggcccgcacc ggctgggaac tggatacgct
     1081 ggtcagtgcg ctcatgacag cgcttcccga ccatgccgcc agtcccctga tgacccgcct
     1141 gcaggacgag ctgtgcacgg aatctgtctg ggggcaggcc cgtgaacagt ttaccggtgc
     1201 ggtggaccgg atatttgaca cggtggagag cgtctgtgtt gcctctgttg cacgcacagt
     1261 tctgcgtgcc gtccgtgaca cggtggtctc tgttgcccgc gcggtatgga actggatttt
     1321 cttctgaaca gtctgccagg ggaaatgtct gattacattg cgttaaccaa ctgaaaacat
     1381 gatacaaaca cctcatgccc ctcctgtttc tcttgcgtga ctgctctact gttaatagaa
     1441 taaaacgatc gataaaacag gtatcgatcg tttatatcga tcgtttatag tggttaatgg
     1501 cctgcgaaaa ggcagctgaa tctcttcatc atgcagaacg gaattgcaca caacagactg
     1561 atacttctct gtctgatgaa gggggagagc gtttacgccc gggacgtatc gctgtgcccg
     1621 ataactctgt tttgtacctg ccggtatcca cttttgtggg taccggcttt tttattcacc
     1681 ctctgtaagg aaaagctgat gaaacgacat ctgaacacca gctacaggct ggtatggaat
     1741 cacattacgg gcaccctggt ggtggcctcc gaactggccc gctcacgggg aaaacgcgcc
     1801 ggtgtggcgg ttgcgctgtc tcttgctgct gtcacatcag tcccggcact ggctgctgac
     1861 aaggttgtac aggcgggaga aaccgtgaac gatggaacac tgacaaatca tgacaaccag
     1921 attgtcttcg gtacggccaa cggaatgacc atcagtaccg ggctggaact ggggccggac
     1981 agtgaagaaa acaccggtgg gcaatggata cagaatggcg ggatagccgg aaacaccact
     2041 gtcaccacaa atggtcgtca ggtcgtgctg gaggggggaa cagccagtga tacggttatt
     2101 cgtgacggcg ggggacagag cctgaacgga ctggcggtga acaccacact gaataacaga
     2161 ggcgagcagt gggtgcatga gggcggggtt gccaccggta caattatcaa ccgcgacggt
     2221 taccagagcg ttaaaagtgg cgggctggca acaggaacca tcatcaacac cggcgcagaa
     2281 ggcggccctg attctgacaa ctcgtatacg ggtcagaagg tccagggaac agcagaatcc
     2341 accaccatca acaaaaatgg acggcagatt atcttatttt ccgggctagc ccgtgacact
     2401 ctcatttacg caggtggtga ccagtcggta cacggaaggg ccctgaatac cacactgaat
     2461 ggcggttacc aatatgtgca cagggacgga cttgcgctga acacggtaat taacgagggg
     2521 ggctggcagg ttgttaaggc aggtggcgct gccggtaaca ccaccataaa tcagaacggt
     2581 gaactgaggg tacatgccgg cggggaagcc actgcagtca cccagaacac gggcggtgca
     2641 ctggttacca gtactgctgc aactgtcatc ggcacaaacc gtctggggaa tttcacggtg
     2701 gaaaacggta aggctgacgg tgttgttctg gaatccggcg gtcgtctgga tgtactggag
     2761 agccattcag cacagaatac cctagtggat gacggcggta ccctggcagt gtctgccggc
     2821 ggtaaggcga caagtgtcac cataacatcc ggtggtgccc tgattgcaga cagtggtgcc
     2881 actgttgagg ggaccaatgc cagcggtaag ttcagtattg atggcacatc cggtcaggcc
     2941 agcggcctgc tgctggaaaa tggcggcagc tttacggtta atgccggggg acaggctggc
     3001 aacaccactg tcggacatcg tggaacactg acgctggctg ccgggggaag tctgagtggc
     3061 agaacacagc tcagtaaagg cgccagtatg gtactgaatg gtgatgtggt cagtaccggc
     3121 gatattgtta acgcagggga gattcgcttt gataatcaga cgacaccgaa tgccgcgctg
     3181 agccgtgctg ttgcaaaaag taactccccg gtaacgttcc ataaactgac caccacgaac
     3241 ctcaccggcc agggcggcac catcaatatg cgtgttcgcc ttgatggcag caatgcctct
     3301 gaccagctgg tgattaatgg tggtcaggca accggcaaaa cctggcttgc gtttacaaat
     3361 gtcggaaaca gcaacctcgg ggtggcaacc accggacagg gtatccgggt tgtggatgca
     3421 cagaatggcg ccaccacaga agaaggtgcg tttgccctga gtcgcccgct tcaggccggc
     3481 gcctttaact acaccctgaa ccgtgacagc gatgaagact ggtacctgcg cagtgaaaat
     3541 gcttatcgtg ctgaagtccc cctgtataca tccatgttga cacaggcaat ggactatgac
     3601 cggattctgg caggctcccg cagccatcag accggtgtaa acggtgaaaa taacagcgtc
     3661 cgtctcagca ttcagggcgg tcatctcggt cacgataaca acggcggtat tgcccgtgga
     3721 gccacgccgg aaagcagcgg cagctatggc ttcgtccgtc tggagggtga cctgctcaga
     3781 acagaggttg ccggtatgtc tctgacgaca ggggtgtatg gtgctgcagg ccattcttcc
     3841 gttgatgtta aggatgatga cggttcccgc gccggcacgg tccgggatga tgccggcagt
     3901 ctgggcggat acctgaatct ggtacacaca tcctccggcc tgtgggctga cattgtggcc
     3961 cagggaaccc gtcacagcat gaaagcgtca tcggacaata acgacttccg cgcccggggc
     4021 tggggctggc tgggctcact ggaaaccggt ctgcccttca gtatcactga caatctgatg
     4081 ctggagccac aactgcagta cacctggcag ggactctccc tggatgacgg ccaggataac
     4141 gccggttatg tgaagttcgg gcatggcagt gcacaacatg tgcgtgccgg tttccgtctg
     4201 ggcagccaca acgatatgac ctttggtgaa ggcacctcat cccgtgacac cctgcgcgac
     4261 agtgcaaaac acagtgtgag tgaactgccg gtgaactggt gggtacagcc ttctgttatc
     4321 cgcaccttca gctcccgggg tgacatgagc atggggacag ccgcagccgg cagtaacatg
     4381 acgttctcac cgtcccggaa tggcacgtca ctggacctgc aggccggact ggaagcccgt
     4441 atccgggaaa atatcaccct gggcgttcag gccggttatg cccacagcgt cagcggcagc
     4501 agcgctgaag gctataacgg tcaggctacg ctgaatatga ctttctgatt atacggtatc
     4561 ccggaaatac gatttcagga aaattcatgc agtaatggga aaggacgctc attgctgttc
     4621 accgcagttc tgaattattg aatttataaa ggtgaatgat gcacgaaaag aacatcgccc
     4681 tgctttgtga tgaagccgac cggcttttgc aactgaatat caatctgctc cggcaaatgg
     4741 ttgatgagcc ggatgtgtta tctgacagta agaacgaaaa cgggctgctt tttgataaac
     4801 ggaaagcgct gaaaagaatt gaggagctgg agggcgaaca aatcaaaacc gcccgcaggg
     4861 agatggtgct ggctgtggtc ggcacgatga aggcgggcaa atcaaccacc atcaacgcca
     4921 ttgtggggca ggaaattctg cccaaccgta accgccccat gacctcggta ccgacattga
     4981 tccgccacgt tcccggaaaa acggaaccgg tcctccatct ggaacatatt cagcccgtcc
     5041 gcaatttatt aatcacactg caggaaaaac tcgccacccc ggcagggcag caagtcgcac
     5101 agtccctgca gcaaaccggg gatacccgcg aactactgga tattctggcg gatgatgtct
     5161 ggcttaaaaa tgaataccac ggggaagacg aaatctttac cggactggca tcgttaaatg
     5221 acctggttcg tcttgctgcg gcaatgggga ctgaatttcc ttttgacgaa tacgcagaag
     5281 tgcagaaact accggtgatc gacgtggcgt tcagccacct ggtgggaatg gatgcatgtc
     5341 agggaacact cacactgctg gatacccccg gccctaatga agccggacaa ccacagatgg
     5401 aaatgatgat gcgggatcaa ctacagaaag cctctgcggt gctggcggtg atggattaca
     5461 cccagatgaa ctcgaaagcc gatgaggagg ttcgtaaaga gcttaatgcc attgctgacg
     5521 tctcagccgg tcgtctgttt gtgctggtca ataaattcga tgaaaaagat cgcaatggtg
     5581 atggtgcgga tgccgtacgc cagaaggtgc cagcaatgct gaacagcgat gtgctgcccg
     5641 cctcccgcgt ttatcccggc tcatcacgtc aggcgtacct ggctaaccgt gcgcttcatg
     5701 agttacgaaa aaacggtgcg cttccggttg atgaagcctg ggtcgatgat tttatcaggg
     5761 aagctttcgg acctatggtg gaagaggacg actggaaaga cagcactaag gtaaataaaa
     5821 aggcagaaaa actctggaat atatctctga tcgatcaact gataacggaa gtcatacaga
     5881 gctcccattc cagagcggcg gcactggcgg ttgactctgc cgccgcaaaa ctgatgcaga
     5941 atgcagaaaa tgtcagcgaa tatctgtcat tacgtcatca ggggctgcag caaagcattc
     6001 agtcactaca ggcgcatatt accagcctgc tggcggatat ccaggagatt gaggaatgtc
     6061 agaatcaggt gaccggtgat gtcagaatgg ccatggagga catcaatacc aaaaccgggg
     6121 aattactgac gaaggtctgt gcctcactgg aggaggagct gaacgactat ttcagaagcg
     6181 gtaaacgcaa agaacagcaa atgctggagg aagaaaattc agcgcagccc cgggagagaa
     6241 acgcattcgc tttctttcac gatattttcg gaaccggaaa tcagcatgac aggatgcggg
     6301 actttgatcc ggacagcccc gaaataaaat tcagtgatcg caggg
//
LOCUS       DAZFDC010000195         6099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFDC010000195 DAZFDC010000000
VERSION     DAZFDC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.195"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..177
                     /gene="ylcI"
                     /locus_tag="ACHJ48_004458"
     CDS             <1..177
                     /gene="ylcI"
                     /locus_tag="ACHJ48_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4709869.1"
                     /translation="TRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEACRRRLT
                     SEKRAYTSIKSDEE"
     gene            complement(342..548)
                     /gene="ybcW"
                     /locus_tag="ACHJ48_004459"
     CDS             complement(342..548)
                     /gene="ybcW"
                     /locus_tag="ACHJ48_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFO4709870.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            834..1244
                     /gene="ybcV"
                     /locus_tag="ACHJ48_004460"
     CDS             834..1244
                     /gene="ybcV"
                     /locus_tag="ACHJ48_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO4709871.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            1536..1829
                     /gene="bor"
                     /locus_tag="ACHJ48_004461"
     CDS             1536..1829
                     /gene="bor"
                     /locus_tag="ACHJ48_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO4709872.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(1920..2102)
                     /gene="rzoD"
                     /locus_tag="ACHJ48_004462"
     CDS             complement(1920..2102)
                     /gene="rzoD"
                     /locus_tag="ACHJ48_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO4709873.1"
                     /translation="MRKLKMMLCMMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2319..2795)
                     /locus_tag="ACHJ48_004463"
     CDS             complement(2319..2795)
                     /locus_tag="ACHJ48_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO4709874.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(2782..3087)
                     /locus_tag="ACHJ48_004464"
     CDS             complement(2782..3087)
                     /locus_tag="ACHJ48_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO4709875.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(3409..4098)
                     /locus_tag="ACHJ48_004465"
     CDS             complement(3409..4098)
                     /locus_tag="ACHJ48_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4709876.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(4095..4235)
                     /locus_tag="ACHJ48_004466"
     CDS             complement(4095..4235)
                     /locus_tag="ACHJ48_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4709877.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(4232..4594)
                     /gene="rusA"
                     /locus_tag="ACHJ48_004467"
     CDS             complement(4232..4594)
                     /gene="rusA"
                     /locus_tag="ACHJ48_004467"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO4709878.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGNE"
     gene            complement(4591..4881)
                     /gene="ybcO"
                     /locus_tag="ACHJ48_004468"
     CDS             complement(4591..4881)
                     /gene="ybcO"
                     /locus_tag="ACHJ48_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO4709879.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(4874..5044)
                     /locus_tag="ACHJ48_004469"
     CDS             complement(4874..5044)
                     /locus_tag="ACHJ48_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4709880.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(5044..5499)
                     /locus_tag="ACHJ48_004470"
     CDS             complement(5044..5499)
                     /locus_tag="ACHJ48_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFO4709881.1"
                     /translation="MNLPQDGIKLHRGNFTAVGQQIQTYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLGTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(5496..5594)
                     /locus_tag="ACHJ48_004471"
     CDS             complement(5496..5594)
                     /locus_tag="ACHJ48_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709882.1"
                     /translation="MRPQSELLTLSQMQKCTCDVLHPAFDLCGGEA"
     gene            complement(6001..>6099)
                     /locus_tag="ACHJ48_004472"
     CDS             complement(6001..>6099)
                     /locus_tag="ACHJ48_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709883.1"
                     /translation="GIVFKHYPLMKVISAQQAISALKYMVDGEVYF"
BASE COUNT         1631 a         1467 c         1334 g         1667 t
ORIGIN      
        1 acccgcagaa caacaacccg caacatccgc tttcctaacc aaatgattga acaaattaac
       61 atcgctcttg atcaaaaagg gtccggaaat ttctcagcct gggtcattga agcctgccgt
      121 cggaggctaa cgtcagaaaa gagagcatat acatcaatta aaagtgatga agaatgaaca
      181 tcccgcgttc ttccctccga acaggacgat attgtaaatt cacttaatta cgagggcatt
      241 gcagtaattg agttgcagtt ttaccacttt cctgacagtg acagactgcg tgttggctct
      301 gtcacaggtt aagtagtttg aatgattagc agttatggtg atcagtcaac caccagggaa
      361 taatccttca tattattatc gtgcttcacc aacgctgcct caattgccct gaatgcttcc
      421 agagacacct tatgttctat acatgcaatt acaacatcag ggtaactcat agaaatggtg
      481 ctattaagca tattttttac acgaatcaga tccacggagg gatcatcagc agattgttct
      541 ttattcattt tgtcgctcca tgcgcttgct cttcatctag cggttaaaat attacttcaa
      601 atctttctgt atgaagattt gagcacgttg gccttacata catctgtcgg ttgtatttcc
      661 ctccagaatg ccagcaggac cgcactttgt tacgcaacca atactattaa ttgaaaacat
      721 tcctaatatt tgacataaat catcaacaaa acacaaagag gtcagaccag attgaaacga
      781 taaaaacgat aatgcaaact acgcgccctc gtatcacatg gaaggtttta ccaatggctc
      841 aggttgccat ttttaaagaa atattcgatc aagtgcgaaa agatttaaac tgtgaattgt
      901 tttattctga actaaaacgt cacaatgtct cacattatat ttactatcta gccacagata
      961 atattcacat tgtgttagaa aacgataaca ctgtgttaat aaaaggactt aaaaaggttg
     1021 tgaatgttaa attctcaaga aatacgcatc ttatagaaac gtcctatgat aggttgaaat
     1081 caagagaaat cacatttcag caatacaggg aaaatcttgc taaagcagga gttttccgat
     1141 gggttacaaa tatccacgaa cataaaagat attactatgc ctttgataat tcattactat
     1201 ttactgagag cattcagaac actacacaaa tctttccacg ctaaatcatc acgtccggtt
     1261 tcttccgtgt cagcaccggg gcgttggcat aatgcaatac gtgtacgcgc taaaccctgt
     1321 gtgcatcgtt attaattatt cccggacact cccgcagagg agtttccctg tcagggctgc
     1381 ggacatagtt aatccgggaa tacagtgacg atccttcgca tctgacatac attaataaat
     1441 attaacaata tgagatttca actcattgtt tagggtttgt ttaattttct acacatacga
     1501 ttctgcgaac tttaaaaagc atcgggaata acaccatgaa aaaaatgcta ctcgctactg
     1561 cgctggccct gcttattaca ggatgtgccc aacagacgtt tactgttcaa aacaaaccgg
     1621 cagcagtaac accaaaggaa accatcaccc accatttctt cgtctctgga attgggcaga
     1681 agaaaactgt cgatgcagct aaaatttgtg gcggcgcaga aaatgttgtt aaaacagaaa
     1741 cccagcaaac attcgtaaat ggattgctcg gttttattac tttaggcatt tatactccgc
     1801 tggaagcgcg tgtgtattgc tcacaataat tgcatgagct gcccatcgat atgggcagct
     1861 ctatctgcac tgctcattaa tatacttctg ggttccttcc agttgttttt gcatagtgat
     1921 cagcctctct ctgagggtga aataatcccg ttcagcggtg tctgccagtc ggggggaggc
     1981 tgcattatcc acgccggagg cggtggtggc ttcacgcact gactgacaga ctgctttgat
     2041 gtgcaaccga cgacgaccag cggcaacatc atcatgcaga gcatcatttt cagctttcgc
     2101 atcagctaac tccttcgtgt attttgcatc gagcgcagca acatcacgct gacgcatctg
     2161 catgtcagta attgccacgt tcgccagctt cagttctctg gcatttttgt cgcgctgggc
     2221 tttgtaggta atggcgttat cacggtaatg attaacagcc catgacaggc agacgatgat
     2281 gcagatgagc agagcggaga taatcgcggt tattctgctc atacctcact ttctctgacc
     2341 gttccgccag cttctttgaa ttttgcaatc aggctgtcag ccttatgctc gaactgacca
     2401 taaccagcgc ccggcagtga agcccagata ttactgcaac ggtcgattgc ctgacggata
     2461 tcaccgcgat caatcatagg taaagcacca cgctctttaa tctgctgcaa tgccactgcg
     2521 tcctggcttt tgggggagaa gtctttcaaa ccaagctgtt tacggtaagc atcccaccag
     2581 cgtgaaagaa gctgataacg tccggcggct gttgatttga gtttggggtg tagcgtgaca
     2641 agtttgcgag ggtgatcgga gtaatcagta aacagttcgc cgccaacaat aacatcataa
     2701 ccgtggttac gtgtcggttg tcgcccgtta tccgttcctt ctgaccacgc caacatatcg
     2761 aggaaggctt tacgctgagg attaagattt tgcatttttc acccctgtca gtcgttccca
     2821 gaagtacgtc agtgcaaccg aacccatcgc accactaatc cccgctgtcg cgagaatcat
     2881 gtaaatactg aatccacttt cgatgctgat caggccacca ataacaccgg tgaatcctga
     2941 taccactatt tgagccagag catttatcca actccacgtt gctttactct gcttcacatc
     3001 tatcaggtag cggaccagac cgccccaacc tgcgatgatc agcaaaacga gccagaacgc
     3061 tccggcaagg ctctcttttt cgtgcatatg aatagccaat gtttcgccgc cgacaaaagg
     3121 ccgggacgtt aaatgtcaga aatcaggctc acggggtaat ttaacgacaa agcacggagt
     3181 tgatgctccc cacaagcctg gaataaaaaa gccagcatgt agctggcaac agagggctaa
     3241 gcaatatcaa ctcaacagct gaagaccccc tggctggggt aggttggaag gctactcacc
     3301 gttcagaaac agaaaagccc aaggctttaa acctcgggct tgaatttgga ttactgccag
     3361 tgcgtacaac attggcaaaa tatcagattt acataaaata tatgattttt aatccagttt
     3421 tgcaatatct tgctgtgaaa atgtggtctt ttgttttgaa cgtgttttcg ttaaaagcaa
     3481 taaagcttgg ctatcaagct gtagaaaaat gtgtttcatt gcaacccagc gttcagtaaa
     3541 tgtctcagac cagttttttg atgtcactcc caccagtgat gccagctcct ggtattcata
     3601 ggtcttacgc cctgccagct cgttcttcac atcctgtgcc gccagccaga tcaacttctt
     3661 caaacgttcc agtgtcttac ctgcaatttt cctggtaccc aacagagtct taaactcgct
     3721 ccatgcccac tgcgttatgg tgacctgatg ttcccagcga acactttcgc tgtaactcca
     3781 cagcaaccac gctttctgat gttcttcgag agacagaacc gcgcggcgcc atgacgaggt
     3841 tgagaactca acctggctga ccagtgcaat ggatgaacct tttgcgtacg actgcttacc
     3901 ggaaatcggc ggattatcca gcgtaatcat cctgccagtt acctcatcca gaatgcgcgg
     3961 cttctttcgt ttgtatgtac cagtatcaaa ttgtgcatgc tccagccagg cttcaagctg
     4021 tcctttcgtt gctccgctca aatcagcggt agccacaatg agttgctcgc ggacatactg
     4081 taaatattgg gtattcatgc ggcagctcct ttcagtgttt tggcgtaatt cttcagtatc
     4141 cggtaatcgg tcaaaacaga accaggaaaa cgatataagc gcaggcgcat ccagcggtgg
     4201 cgaagacgtt ctgccatata agactcaaac atcattcatt ccccagttcg gtgatggtca
     4261 gctccagctt cccacctttg gtaacgggca tcttcacaac acggtaatca acgacctgag
     4321 catcatccag ccagaaacct gctttggtga gtgcgtcaaa agcggctttt tgcagattat
     4381 ccaggtcacg gcgacggcga tccggcatgt ggcactcaat acggattttc actggcatag
     4441 ccaggccgat atccagcatg gagcctttga tgattcgggc gacgttatca cggtatgcct
     4501 gcccttctgc gctgatgtgc gtgcgcccgc gattatgccg gtagtagcga ttattgctcg
     4561 gaggccaggg taatgtgatg ctgtaggtat tcacgcctta ataaccccct ctttcagcca
     4621 gataacctgt gttctcgcca taccttccag cgcgcattct tttgcatatg cagcatcgac
     4681 aaaatgtgtg cggcggtcga tttcgtcgtg gcaggcagaa catgcaatgg tggcaatcaa
     4741 gtctggcggt ttggtaccgg tgccgcacaa tccagtcagc cggatatgtg ccagtacaga
     4801 cgtttcaggg ttgccattac atacgccagg gattcttact tggcattccc gactacgcgc
     4861 tgcttttctc aaatcagcca tgattcctcc ttgctgccag tcgcaaccat tttttatcaa
     4921 ccaggctggc ggtatatccg agcagtgttg gtatttcgga tggcttcagc tcaggtttac
     4981 gcttacgacg atttggtact ttgtagatgt gtccgttcat gacacgaata agcggtgtag
     5041 ccattacgcc tcctgcttgt cacgcagcag ctggaactcg cagctctgtg gaatagtcag
     5101 gtggcagcca atattcatcg cccaggcttc aaccttacac aggaagacat acatctctcc
     5161 ggtaccaaga tcggaggtat ggcgtaacga ctggatagta gtgatttcgc cggttacgac
     5221 atcaaccagg tccttggttt cataaccgag gtatgtgtgt ttgagagcat cttttaccca
     5281 tgctgaagta gcgaacgatt tccccctgct gatgaggtat tcactgattt cgctgtacca
     5341 catgtggctg agtgcattct gggaaagact gcgtctctcg cgccacggtt taagcaccat
     5401 gcgaaagcat ttgccgtctt ccagataagt ctggatctgc tgaccgacag cggtgaagtt
     5461 accgcgatgt aatttgatgc catcttgtgg gaggttcacg cttcacctcc gcagagatca
     5521 aacgctggat gcaagacatc gcaggtgcat ttctgcatct gtgacaaggt aaggagttca
     5581 gattgtggtc gcatttaagt ccccttaaat gcgcagaagt caccggagtt gttcaagctc
     5641 cggtgacatt attatggaag gttgatacta aaaaatcaaa aattgaatat cattggcgat
     5701 agtctagctt gtgatatcca caagctactt caatgaagcg acgagtaaca ttttgtgtca
     5761 tccatgtttt ttcagttaag tgccacaccc agttcaaaat ttgctgatgt gttgaacatg
     5821 tactaagcgg gatgttgtat tcataagcaa cattgattcc gcaaaatcgg tgtcgtgtca
     5881 tgaccgattc acgttgacct gcgccctgtt gagtgcctgg ggatattctt gctcgatagt
     5941 taatatcata gtagttactt tactttcata ccatagcacg gttgaaaaag tgattattac
     6001 tcaaaaataa acctcaccat caaccatata tttgagagca cttatcgcct gctgggcgga
     6061 tattactttc attaaaggat agtgtttaaa aacaatgcc
//
LOCUS       DAZFDC010000196         6060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFDC010000196 DAZFDC010000000
VERSION     DAZFDC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.196"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            225..1328
                     /gene="ompC"
                     /locus_tag="ACHJ48_004473"
     CDS             225..1328
                     /gene="ompC"
                     /locus_tag="ACHJ48_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO4709884.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1440..2495
                     /gene="apbE"
                     /locus_tag="ACHJ48_004474"
     CDS             1440..2495
                     /gene="apbE"
                     /locus_tag="ACHJ48_004474"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO4709885.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2569..3633
                     /gene="ada"
                     /locus_tag="ACHJ48_004475"
     CDS             2569..3633
                     /gene="ada"
                     /locus_tag="ACHJ48_004475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO4709886.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYHKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3633..4283
                     /gene="alkB"
                     /locus_tag="ACHJ48_004476"
     CDS             3633..4283
                     /gene="alkB"
                     /locus_tag="ACHJ48_004476"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO4709887.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4359..6002
                     /gene="yojI"
                     /locus_tag="ACHJ48_004477"
     CDS             4359..6002
                     /gene="yojI"
                     /locus_tag="ACHJ48_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO4709888.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPSWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELRNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLSLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1442 a         1532 c         1637 g         1449 t
ORIGIN      
        1 tgcttatttc gccattccgc aataatctta aaaagttccc tcgaatttac attttgaaac
       61 atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg ttggattatt
      121 ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc agtatgcagt
      181 ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa gttaaagtac
      241 tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct gaagtttaca
      301 acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac tatttctctg
      361 acgacaagtc tgtagatggc gaccagacct acatgcgtct tggcttcaaa ggtgaaactc
      421 aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag ggcaacagcg
      481 ctgaaaacga aaacaactcc tggacccgtg tggcattcgc aggtctgaaa ttccaggatg
      541 taggttcttt cgactacggt cgtaactacg gcgtagttta cgacgtaact tcctggaccg
      601 acgttctgcc agaattcggt ggtgacacct acggttctga caacttcatg cagcagcgtg
      661 gtaacggttt cgcgacctac cgtaacactg acttcttcgg tctggttgac ggcctgaact
      721 ttgctgttca gtaccagggc aaaaacggta gcgtaagcgg cgaaggcatg actaacaacg
      781 gtcgtgaagc actgcgtcag aacggcgacg gcgttggcgg atctatcact tatgattacg
      841 aaggcttcgg tatcggtgct gcagtttcca gctccaaacg tactgatgat cagaacagcc
      901 cgctgtacat cggtaacggc gaccgtgctg aaacctacac tggtggtctg aaatacgacg
      961 ctaacaacat ctatctggct gctcagtaca cccagaccta caacgcaact cgcgtaggtt
     1021 ccctgggttg ggcgaacaaa gcacagaact tcgaagctgt tgctcagtac cagttcgact
     1081 tcggtctgcg tccgtccctg gcttacctgc agtctaaagg taaaaacctg ggtgtcatca
     1141 atggccgtaa ctacgacgac gaagatatcc tgaaatatgt tgatgttggc gcgacctact
     1201 acttcaacaa aaacatgtcc acctatgttg actacaaaat caacctgctg gacgacaacc
     1261 agttcactcg tgacgctggc atcaacactg ataacatcgt agctctgggt ctggtttacc
     1321 agttctaatc tcgattgata tcgaacaaag ggcctgcggg cccttttttc attgttttca
     1381 gcgtacaaac tcagtttttt ggtgtactct tgcgaccgtt cgcatgagga taatcacgta
     1441 tggaaataag ctttacccgc gtggcactgc tggctgccgc gctcttcttt gttggttgcg
     1501 atcaaaaacc acaacccgcc aaaacccacg ctactgaagt taccgttctt gaaggcaaaa
     1561 ctatgggtac cttctggcgt gccagcatcc cgggcattga cgccaaacgc agcgccgaac
     1621 ttaaagaaaa gattcagacc cagctggacg ccgacgatca gctgctttcg acctataaaa
     1681 aagattccgc gctgatgcgc tttaacgact cgcaaagttt atcgccgtgg ccggtaagtg
     1741 aagcgatggc cgatatcgtc accacctcgc tacgcattgg cgcgaagacc gatggcgcga
     1801 tggatataac cgtcgggccg ctggtgaatc tgtggggttt cggtccggaa caacagccgg
     1861 ttcaaattcc gagccaggaa cagatcgacg cgatgaaagc caaaactggt ttacagcacc
     1921 ttacggtcat taatcagtcg catcagcaat atctgcaaaa agacctgccg gatttatatg
     1981 tcgatctctc caccgtcggc gaaggttatg cggcggatca cctggcacgc ttgatggagc
     2041 aggaagggat ttcccgctat ctggtgtcgg tgggcggcgc gctgaacagc cgtggtatga
     2101 acggtgaagg cctgccgtgg cgggtagcga ttcaaaaacc aaccgataaa gaaaacgcgg
     2161 ttcaggccgt ggtggatatc aacggtcatg ggattagcac ctctggcagc taccgtaact
     2221 attacgaact ggacggcaaa cgtctttccc atgttatcga tccgcaaacc gggcgtccca
     2281 tcgaacacaa tctggtatcc gtgacggtga ttgcaccgac ggcgctggaa gccgacgcct
     2341 gggatacagg tttgatggta ctcgggccgg agaaagccaa agaagttgtt cgccgggaag
     2401 ggctggcggt ttatatgatc accaaagaag gcgatagctt taaaacctgg atgtcaccac
     2461 agtttaaaag cttccttgtc agcgaaaaaa attaaagcgc aagattgttg gtttttgcgt
     2521 gatggtggcc gggcagacta aaggctatcc ttaaccaggg agctgattat gaaaaacgcc
     2581 acatgcttaa ctgacgatca acgctggcaa tctgtcttag cccgcgaccc gaatgccgac
     2641 ggcgaattcg ttttcgccgt gcgcaccaca ggcatctttt gccgtccgtc ttgccgcgcc
     2701 agacatgctt tgcgggaaaa cgtctccttc tacgcaaatg ccagcgaggc actcgccgcc
     2761 ggatttcgcc cctgcaaacg ctgccaacct gataaagcca atgcccagca acatcggttg
     2821 gataaaatca cccacgcgtg tcgactgctg gaacaggaaa cgcctgtaac gttggaagcc
     2881 ttagccgacc aggtggcgat gagtccattt catctacatc ggttgtttaa agcgactacc
     2941 ggaatgacgc ctaaagcctg gcaacaggcc tggcgcgctc gccgtttgcg cgaatcgctg
     3001 gcgaaagggg agagcgtgac gacgtctatt cttaacgccg gattccccga cagcagcagt
     3061 tactaccaca aagccgatga aacgctgggc atgacggcta aacaattccg tcacggtggc
     3121 gaaaatctgg cggtgcgtta cgcgctggct gattgtgagc tgggtcgttg cctggtggca
     3181 gaaagcgagc gggggatttg cgcgatattg ctgggcgatg atgacgcgac attaatcagc
     3241 gagttgcagc agatgtttcc cgctgccgac aacgcgcctg ccgatctgac gtttcagcaa
     3301 catgtgcgtg aagtgatcgc cagcctcaat caacgcgata ctccgctgac gttaccgctg
     3361 gatattcgcg gcactgcttt tcagcaacaa gtctggcagg cactgcgcac gataccttgc
     3421 ggtgaaaccg tcagttatca gcaactggct aacgccatcg gcaaaccgaa agcggtacgg
     3481 gccgttgcca gcgcctgtgc cgccaacaag ctggctatcg taattccctg tcatcgagtg
     3541 gtccgtggtg atggcacact ttccggttac cgctggggcg tgtcgcgtaa agcgcaactg
     3601 ctgcgccgcg aagctgaaaa tgaggagagg taatgttgga tctgtttgcc gatgctgaac
     3661 cgtggcaaga gccactggcg gctggtgcgg taattttacg gcgttttgct tttaacgctg
     3721 cagagcaact aatccgcgat attaatgacg ttgccagcca gtcgccgttt cgccagatgg
     3781 tcacccccgg gggatatacc atgtcggtgg cgatgaccaa ctgtgggcat ctgggctgga
     3841 cgacccatcg gcaaggttat ctctattcgc ccattgatcc gcaaacaaat aaaccgtggc
     3901 ccgccatgcc gcagagtttt cataatttat gtcaacgtgc ggctacggcg gcgggctatc
     3961 cagatttcca gccagatgct tgtcttatca accgctacgc tcctggcgcg aaactgtcgc
     4021 tgcatcagga taaagacgaa ccggatctgc gcgcgccaat tgtttctgtt tctctgggct
     4081 tacccgcgat ttttcaattt ggtggcctga aacgtaatga tccgctcaaa cgtttgttgt
     4141 tggaacatgg cgatgtggtg gtatggggcg gtgaatcgcg gctgttttat cacggtattc
     4201 aaccgttgaa agcggggttt catccactca ccaccgactg ccgctacaac ctgacattcc
     4261 gtcaggcagg taaaaaagaa taaaaataag aattattatt gctgtgcgcg cgaagatgtt
     4321 taaactgcgg gctgtaattc attgtccggg ttttctgcat ggaacttctt gtacttgtct
     4381 ggcggcagta tcgctggcca tttatcagtg tgatggcgct aagcctcgcc agtgcggctt
     4441 taggcattgg cttaattgct tttatcaatc agcgccttat cgaaacggcg gataccagtc
     4501 tgctggtgtt gccggagttt ctgggattat tgctgctgtt gatggcagtc actctcggat
     4561 cgcaactggc gctcaccact ttggggcatc acttcgttta ccgactgcgt agtgaattta
     4621 tcaagcggat tctggatact cacgtcgagc gcattgaaca actcggtagc gcctcgttgc
     4681 tggcgggatt aaccagcgat gtgcgcaata tcaccattgc ttttgtgcgt ctgccggaac
     4741 tggtgcaggg gatcattctc actatcggtt cagcggcgta tctgtggatg ctgtcgggca
     4801 aaatgttgct ggtaacggct atctggatgg cagtcaccat ctggggcggt tttgtgctgg
     4861 tggcgcgggt gtacaaacat atggcgaccc tgcgtgaaac cgaagacaag ctgtacacgg
     4921 attttcaaac tgtacttgaa gggcgcaaag agctgactct gaaccgggaa cgcgccgagt
     4981 atgtgtttaa caacctctac attcctgatg cgcaagagta tcgccatcat attattcgcg
     5041 cagacacctt ccatcttagt gccgtgaact ggtcaaacat catgatgctg ggcgctatcg
     5101 gcctggtgtt ctggatggcg aacagtctcg gttgggctga taccaacgtt gccgcgacct
     5161 attcgttgac gcttttattc ctgcgtacgc cgctgctttc ggcggttggc gcattgccga
     5221 cgctgctgac ggcgcaggtg gcgtttaaca agctgaacaa attcgcgctc gcgcctttca
     5281 aagcagagtt tccgcgcccg caggcgtttc ccagctggca aacgctggag ctgcgtaacg
     5341 tgacgtttgc ttatcaggat aacgcgtttt ccgttggtcc gattaatctc accatcaaac
     5401 gtggcgagct gctgtttctg attggcggca acggtagcgg aaaatcgacg ctggcgatgt
     5461 tgttgacggg cttgtatcag ccacaaagcg gcgaaatctt gctggatggc aaacctgtca
     5521 gcggcgaaca accggaagat tatcgcaaac tgttttcggc agtgtttacc gatgtctggc
     5581 tgtttgatca actgctgggg ccggagggta aacccgctaa cccgcaactg gttgagaagt
     5641 ggctggcgca gctgaaaatg gctcataagc ttgagttaag aaacgggcgt attgttaacc
     5701 tgaagttatc aaaagggcag aaaaaacgcg tggcgctgtt gctggcgctg gcagaagaac
     5761 gcgatattat cctgctggat gaatgggcgg cggatcagga tccacacttc cgtcgtgagt
     5821 tttatcaggt gttgctgtcg ctgatgcagg agatgggtaa aactattttc gccatcagtc
     5881 atgatgatca ttactttatc cacgccgacc gcctgctgga aatgcgcaac gggcaactta
     5941 gcgagctgac gggcgaagag cgcgatgccg cttcgcgtga tgccgttgcc cggacggcat
     6001 aatattacga cgctgacctg gcggactgct gccgccaggt caatatgcta aaaatccctt
//
LOCUS       DAZFDC010000197         6039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFDC010000197 DAZFDC010000000
VERSION     DAZFDC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.197"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..220
                     /locus_tag="ACHJ48_004478"
     CDS             <1..220
                     /locus_tag="ACHJ48_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4709889.1"
                     /translation="PENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFW
                     IDRISTDDGGSCHLWLGRGVPPAVNRRR"
     gene            232..810
                     /locus_tag="ACHJ48_004479"
     CDS             232..810
                     /locus_tag="ACHJ48_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4709890.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETRVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            807..1202
                     /locus_tag="ACHJ48_004480"
     CDS             807..1202
                     /locus_tag="ACHJ48_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4709891.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1210..1950
                     /locus_tag="ACHJ48_004481"
     CDS             1210..1950
                     /locus_tag="ACHJ48_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4709892.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGNGDPYANPLSDNDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWAATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1966..2388
                     /locus_tag="ACHJ48_004482"
     CDS             1966..2388
                     /locus_tag="ACHJ48_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4709893.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2370..2804
                     /locus_tag="ACHJ48_004483"
     CDS             2370..2804
                     /locus_tag="ACHJ48_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4709894.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2797..5346
                     /locus_tag="ACHJ48_004484"
     CDS             2797..5346
                     /locus_tag="ACHJ48_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4709895.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGEEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFAAGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            5343..5672
                     /locus_tag="ACHJ48_004485"
     CDS             5343..5672
                     /locus_tag="ACHJ48_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4709896.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            5672..>6039
                     /locus_tag="ACHJ48_004486"
     CDS             5672..>6039
                     /locus_tag="ACHJ48_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4709897.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGN"
BASE COUNT         1404 a         1484 c         1975 g         1176 t
ORIGIN      
        1 ccctgaaaat atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct
       61 gtttgtccgg actgatgatg tgcggcaact gcggcgtgga gacacgctga ccatcggtga
      121 ggaaaacttc tggatagacc ggatttcgac ggatgatggc ggaagctgtc atctctggct
      181 tgggcggggc gtaccgcctg ccgttaaccg tcgtcgctga aggggggatg tatggccata
      241 aaaggtcttg agcaggccgt tgaaaacctc agtcgtatca gcaaaacggc ggtgcctggt
      301 gcgtcagcaa tggccattaa ccgcgttgct tcatccgcga tatcgcagtc ggcgtcacag
      361 gttgcccgtg agacaagagt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg
      421 gccacggtta aaaatccgca ggccagaatc aaggttaacc ggggggattt gcccgtaatc
      481 aagctgggta acgcgcgggt tgtcctgtcc cgacgcaggc gtcgtaaaaa ggggcagcgt
      541 tcatccctga aaggtggcgg cagcgtgctt gtggtgggaa accgtcgtat tcccggcgcg
      601 tttattcagc aactgaaaaa tggccggtgg catgtcatgc agcgtgtggc cgggaaaaac
      661 cgttacccca ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa
      721 cagaatattg agcggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag
      781 catcaactga gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg
      841 atgcactgga gaagcatgac accggggcga cgttttttga tggtcgcccc gctgtttttg
      901 atgaggcgga ttttccggca gttgccgttt atctcaccgg cgctgaatac acgggcgaag
      961 agctggacag cgatacctgg caggcggagc tgcatatcga agttttcctg cctgctcagg
     1021 tgccggattc agagctggat gcgtggatgg agtcccggat ttatccggtg atgagcgata
     1081 tcccggcact atcagatttg atcaccagta tggtggccag tggctatgac taccggcgcg
     1141 acgatgatgc gggcctgtgg agttcagccg atctgactta tgtcattacc tatgaaatgt
     1201 gaggacgata tgccaacacc aaatcctctg gcaccggtga aaggggccgg gaccacactg
     1261 tgggtttata acgggaacgg cgacccatat gcaaacccgc tttcagacaa tgactggtcg
     1321 cgtctggcaa aggttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac
     1381 agttatctcg atgatgaaga tgcggactgg gccgcgaccg gacaggggca gaaatccgct
     1441 ggagatacca gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg
     1501 gcgtggttta atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc
     1561 gatgtgttcc gcggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaagtgatc
     1621 acccgcacgg tgaaagtcac caacgtggga cgtccgtcga tggcagaaga tcgcagcacg
     1681 gtaacagcgg caaccggcat gaccgtgacg cctgccagca cctcggtggt gaaagggcag
     1741 agcacgacgc tgaccgtggc attccagccg gaaggcgcaa ccgacaagag cttccgtgcg
     1801 gtgtctgcgg ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa
     1861 ggtgttgctg caggcaaggt caacattccg gtcgtatccg gtaatggtga gtttgctgcg
     1921 gttgcagaaa tcaacgtcac cgccagttaa tccggagaat cagcgatgtt cctgaaaacc
     1981 gaatcatttg aacataacgg cgtgaccgtc acgctttctg aactgtcagc cctgcagcgt
     2041 attgagcatc tcgccctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg
     2101 aagtttactg tggaagacgc catcagaacc ggcgcttttg tggtggcgat gtccctgtgg
     2161 cataaccatc cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag
     2221 caggaagtgc ttaccacctg gcccgcggag gcaatttctc atgctgaaaa cgtggtgtac
     2281 cggctgtccg gtatgtatga gtttgtggtg aatgatgccc ctgaacaggc agaggacgcc
     2341 gggcctgcag agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga
     2401 aactggcgcg cgagatgggg cgacccgact ggcgtgccat gcttgccggg atgtcatcca
     2461 cggagtatgc cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg
     2521 atatgcactt ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggata
     2581 tgcatccgct ggatttcagt ctgctgaacc ggcgtgaggc tgacgaagag cctgaagatg
     2641 atgtgctgat gcagaaagcg gcagggcttg ctggaggcgt tcgttttggc ccggacggga
     2701 atgaagttat ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga
     2761 tgacagtatc agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg
     2821 gtcgttgatt taagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt
     2881 catttttccg gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgatg
     2941 agccggcagg cgctggctgc acagaaagcg ggaatttccg tcgggcagta taaagccgcc
     3001 atgcgtatgc tgcctgcgca gttcactgac gtggccacgc agcttgcagg tgggcaaagt
     3061 ccgtggctga tcctgctgca acaggggggg caggtgaagg actccttcgg cgggatgatc
     3121 cccatgttcc gggggcttgc cggtgcgatc accctgccga tggtgggggc cacctcgctg
     3181 gcggtggcga ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat
     3241 ttcaacaaaa cgctggtcct ttccggcaat caggccgggc tgacggcaga tcgtatgctg
     3301 gtcctgtcca gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc
     3361 agcgcactgg ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt
     3421 gtggcgcgtt tctcctctgc atcaggcgtg gaggtggaca aggtcgctga agccttcggg
     3481 aagctgacca ctgacccgac gtcggggctg acagcgatgg cgcgccagtt ccataacgtg
     3541 acggcggagc agattgcgta tgttgctcag ttgcagcgtt ccggcgaaga agccggggca
     3601 ttgcaggcgg cgaacgaggc cgcgacgaaa gggtttgatg accagacccg ccgcctgaaa
     3661 gagaacatgg gcacgctgga aacctgggca gacaggacag cacgggcatt caaatccatg
     3721 tgggatgcgg tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca
     3781 gaggccgcgt ttaagaaagc ggacgatatc tggaatctgc gcaaggatga ttattttgtt
     3841 aacgatgaag cgcgggcgcg ttactgggat gatcgtgaaa aggcccgtct tgcgcttgaa
     3901 gccgcccgaa agaaggctga gcagcagagt caacaggaca aaaatgcgca gcaacagagc
     3961 gataccgaag cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacgcctg
     4021 cagacgccgc tggagaaata taccgcccgt caggaagaac tgaataaggc actgaaagac
     4081 gggaaaatcc tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggactatgaa
     4141 gcgacgctga aaaagccgaa acagtccggc gtgaaggtgt ctgcgggcga tcgtcaggaa
     4201 gacagtgctc atgctgccct gctgacgctt caggcagaac tccggatgct ggagaagcat
     4261 gccggagcga atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag
     4321 ttcgcggtac tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg
     4381 ctggcgcata aagacgagac gctggagtac aaacgccagc tggctgtact tggcgacaag
     4441 gtcacctatc aggagcacct gaacgcgctg gcacagcagg cggataaatt cgcacagcaa
     4501 caacgggcaa aacgggccgc cattgatgca aaaaaccggg ggctgactga ccggcaggca
     4561 gcgcgggaag ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg
     4621 aataacgtca tgtcagagca gaaaaagacc tgggcagctg aagaccagct tcgcgggagc
     4681 tggatggcag gcctcaggtc aggctggagt gagtgggaag agagcgccac ggacagtatg
     4741 tcgcaggtta aaagtgctgc cacgcagacc tttgatggta ttgcacagaa tatggcggcg
     4801 atgctgaccg gcagtgaaca gaactggcgc agcttcaccc gttccgtgct gtccatgatg
     4861 acagaaattc tgcttaagca ggcaatggtg gggattgtcg gaagtatagg cagcgccatt
     4921 ggcggcggcg catcagcgtc aggcggtaca gccattcagg cagctgcggc gaaattccat
     4981 tttgcggccg gagggtttac gggaaccggc ggcaaatatg agccagcggg gattgttcac
     5041 cgtggtgaat ttgtcttcac gaaggaggca accagccgga ttggcgtagg aaatctttac
     5101 cggctgatgc gcggctatgc caccggcggt tatgtcggta caccgggcag catggcggac
     5161 agccggtcgc aggcgtccgg gacgtttgag cagaataacc atgtggtgat taacaacgac
     5221 ggcacgaacg gtcagatagg gccacaggca ctgaaggcgg tttatgacgt agcccgtaag
     5281 gcggcaatgg atgttgtgac cgggcagatg cgcgatggtg gtctgttctc cggaggtgga
     5341 cgatgaaaac cttccgctgg aaagtgaaac ccggtatgga tgtggcttcg gccccttccg
     5401 taagaaaggt gcgctttggt gatggctatt cccagcgagc gcctgccggg ctgaatgccg
     5461 acctgaaaac gtacagcgtg acgctttctg ttccccgttg ggaggccacg gcgcttgagt
     5521 cgtttctggc tgagcacggg ggctggaagg cctttctgtg gacgccgcct tatgagtggc
     5581 ggcagataaa ggtgacctgc gcaaaatggt cgtcgcgggt cagtatgctg cgtgttgagt
     5641 tcagcgcaga gtttgaacag gtggtgaact gatgcaggat atccggcagg aaacactgaa
     5701 tgaatgcacc cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg atctgacaga
     5761 ggtcggtgga gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac
     5821 ctggcagggg cgacagtatc agccgtatcc cattcagggg agtggttttg aactgaatgg
     5881 caaaggcacc agtacgcgcc ccacgctgac ggtttctaac ctgtacggta tggtcaccgg
     5941 gatggcggaa gatctgcaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc
     6001 ccgttttctg gatgcggtga acttcgtcaa cggaaacag
//
LOCUS       DAZFDC010000198         6027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFDC010000198 DAZFDC010000000
VERSION     DAZFDC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.198"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            34..1104
                     /gene="ygjJ"
                     /locus_tag="ACHJ48_004487"
     CDS             34..1104
                     /gene="ygjJ"
                     /locus_tag="ACHJ48_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO4709898.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            1121..3472
                     /gene="ygjK"
                     /locus_tag="ACHJ48_004488"
     CDS             1121..3472
                     /gene="ygjK"
                     /locus_tag="ACHJ48_004488"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO4709899.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYNIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVLEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     DSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHFYMLYNDFFRKQ"
     gene            3998..6016
                     /gene="fadH"
                     /locus_tag="ACHJ48_004489"
     CDS             3998..6016
                     /gene="fadH"
                     /locus_tag="ACHJ48_004489"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO4709900.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT         1442 a         1630 c         1693 g         1262 t
ORIGIN      
        1 ctgtactttt tctttagctc atggattaac acaatgaaat taatcactgc accatgcaga
       61 gcattacttg ctctgccgtt ttgctacgcc ttttctgcgg caggagaaga agcacgtccg
      121 gcagaacatg acgacacaaa aacccccgca attacctcga catcttctcc ttcatttcgt
      181 ttttacggcg aattaggggt tggtggatat atggatttag agggtgagaa taaacataaa
      241 tacagcgacg gcacctatat tgaaggtggc ctggagatga agtacggctc ctggttcggc
      301 ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc ctgggtgcct
      361 gaccatagct ggggcggttt cgaaggtggg attaaccgtt tctatggcgg ttatcgaacc
      421 aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga tgacctgcaa
      481 tggtggggcg acttcacacc cgatctgggc tacgtcattc ccaatacccg cgacattatg
      541 accgcgctga aggtgcagaa cttaagcggc aactttcgtt atagcgtcac cgccacacct
      601 gccggacatc atgacgaaag caaagcatgg ctacattttg gcaagtacga tcgctacgac
      661 gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct tgccgaaggc
      721 atcacctgga tgaatggtct ggaaattacc gacggcacag gacaactcta tctcacggga
      781 ctgcttaccc ctaactttgc cgctcgcgcc tggcaccata ccggacgtgc cgacgggctg
      841 gacgtaccgg gaagtgaaag cgggatgatg gtgagcgcca tgtatgaagc gttaaagggc
      901 gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc tgacgatgaa
      961 accacctctt tcatgcagtt tggtatctgg tacgaatatg gcggcggacg tttcgcaacg
     1021 gcttttgata gccgcttcta catgaaaaat gcctctaacg atcccagcga ccaaatcttc
     1081 ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaatca aaactatttt
     1141 aacgccagta acctgcgctc tgctgataag tttttccgcc catgccacta acgccgacaa
     1201 ttataaaaac gtgattaacc gtactggtgc gccgcagtac atgaaggatt acgattacga
     1261 cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc atctgttgcc
     1321 agacggcccg aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg aagaatacat
     1381 caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca agaaagtcaa
     1441 cttcacgctg gaggcataca atattcccgg cgcgctggtg caaaaactga cagcaaaaga
     1501 tgtgcaggtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac tggaaactaa
     1561 aatcatcagc aataaaccgc tggatctggt gtgggatggc gaactgctgg aaaaactgga
     1621 agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc ctgactacca
     1681 gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag tgcgcgccac
     1741 ctgggatctg ctgacctccg gtgaatcaga atatcaggtg cataaatccc tgccggtgca
     1801 gactgaaatc aacggcaatc gctttaccag taaggcgcat atcaacggtt cgacgacgct
     1861 ctataccacc tattcccatc tgctgacctc tcaggaagtt agcaaagagc aaatgcagat
     1921 ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac gctgggaaga
     1981 gtatctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacac gcgtcgcggt
     2041 gaaagcgatc gaaacgctca acggtaactg gcgctcgcct gggggtgcgg tgaaatataa
     2101 caccgtcacg ccgtcggtga ccgggcgctg gttctccggc aatcagacct ggccgtggga
     2161 tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca aagagaatat
     2221 ccgcgcggtc ttctcctggc agatccagcc tggcgacagc gtgcgtccgc aggatgtggg
     2281 ctttgtcccc gacctgatag cgtggaatct tagccccgag cgtggtggcg atggtggcaa
     2341 ctggaacgaa cgaaatacca aacccagcct tgccgcctgg tcggtgatgg aagtgtacaa
     2401 cgtcacccag gataaaacct gggtggcaga gatgtacccg aaactggtgg cctatcacga
     2461 ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg gcgcgacccg
     2521 cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaga aaggcaacaa
     2581 agaagagacg cagtctgggc tgaacaacta cgcccgcgtg ttggagaaag gccagtacga
     2641 cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg atgatgccgc
     2701 cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgcaagcg gcggcaaacg
     2761 tagcgactgg acagtgaaat tcgccgaaaa ccgcagtcag gacggaacgt tgctggggga
     2821 ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata accattatct
     2881 ggcggagatg gcgacgattc tcggtaaacc ggaagaggct aagcgctatc gccagttggc
     2941 acagcagctc gcggactaca tcaacacctg tatgttcgac ccggctacgc agtacttcta
     3001 tgacgtgcgt attgaagata aaccgctggc gaacggctgc gcgggcaaac cgattgttga
     3061 gcgcggtaaa gggccggaag gctggtcgcc gctgtttaac ggtgcggcaa cgcaggccaa
     3121 tgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaatacct ttgttccgct
     3181 gggaacggcg gcgttaacca atccggcctt tggcgctgat atctactggc gcgggcgcgt
     3241 atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt atcgcgatga
     3301 tgccctgaaa ctggcggata cgttcttccg gcacgccaaa gggttaaccg ccgatggccc
     3361 aattcaggaa aattacaacc cgctgacagg cgcacagcaa ggcgcaccaa atttctcctg
     3421 gagtgccgca catttttata tgttgtataa cgattttttc cgtaagcaat aatttgcaac
     3481 ggctggcgga tgcggcgtga acgccttatc cgccctacat attcgttccc gtaggctgga
     3541 taagacgcga caagcgtcgc atccggcatc tgcacctcgc caccggcgga tgctgcgtga
     3601 acgccttatc cgccctacat gttcgttccc gtaggctgga taagacgcga caagcgtcgc
     3661 atccggcatc tgcacctcgc cactggcgga tgcggcgtga acgccttatc cgccctacat
     3721 gttcgttccc gtaggctgga taagacccga caagcgtcgc atccggcgtc tgcaccgcgc
     3781 cactggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc ccaggtcgga
     3841 tagggcccgg caagcgtcgc atccggcgtt accaccgggc gtattctttt tgaatcccat
     3901 cacaaacccc gcactcccct tttccctttt ctccggcgac ggctaaatta gaactcatcc
     3961 gaccacataa caattatttt acatactgga caattttatg agctacccgt cgctgttcgc
     4021 cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggtt caatgcacac
     4081 cgggctggag gaatacccgg acggtgctga acggctggca gcgttttatg ccgaacgcgc
     4141 ccgtcacggc gtggcgctga ttgttagcgg cggcattgca ccagatttaa caggcgttgg
     4201 catggaaggc ggcgcaatgc tcaatgacgc cagccagatc ccacaccatc gcaccattac
     4261 cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata ccgggcgcta
     4321 cagctaccaa ccgcatctgg tcgcaccgtc cgcattgcag gcccccatca accgtttcgt
     4381 tcctcatgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg cccgctgcgc
     4441 gcaactggca cgggaggcag gatacgacgg tgtagaggta atgggttccg aagggtattt
     4501 gatcaacgaa tttctgacgc tgcgcaccaa tcagcgcagt gaccagtggg gcggcgatta
     4561 ccacaaccgg atgcggtttg ccgttgaagt ggtgagtgcg gtacgcgaac gcgttggcaa
     4621 cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaaggcg gcgggacttt
     4681 tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca ttatcaacac
     4741 cggcattggc tggcatgaag cgcgtattcc gaccatcgcc acgcccgtgc cacgcggcgc
     4801 atttagctgg gtcacgcgca aactgaaagg tcacgtctcg ctgccgctgg tgaccaccaa
     4861 ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg ccgatatggt
     4921 atcgatggcg cgaccgtttc ttgcggatgc ggaactgctg tcaaaagcgc aatcagggcg
     4981 agccgatgag atcaacacct gcattggctg caaccaggcg tgtctcgatc agatcttcgt
     5041 tggcaaggtc acctcgtgtc tggtgaatcc tcgcgcctgc catgaaacca aaatgccgat
     5101 ccttcccgcc atgcagaaaa aaaatctggc ggtggtcggt gcgggacctg ctgggctggc
     5161 gtttgccatt aacgcggcgg cgcgtggtca tcaggtaacg ttgtttgacg cgcacagtga
     5221 gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt tttacgaaac
     5281 gctgcgctat taccgccgga tgatcgaagt gacgggcgtg acgctaaaac tcaatcacac
     5341 cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg ggatcgtgcc
     5401 gcgcactccg cccatcgacg ggatcgatca tccgaaggta ttgagttatc tcgatgtact
     5461 gcgcgacaaa gcgccggtgg gcaacaaagt tgccatcatc ggttgtggcg ggattggttt
     5521 tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata tcgccgggtt
     5581 ctgtaatgaa tgggggatcg acagcagcct acaacaggct ggtggcttaa gcccgcaggg
     5641 aatgcagatc ccgcgtagcc cacggcagat tgtgatgctc cagcgcaaag ccagcaaacc
     5701 gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc tctcgcgggg
     5761 tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc tgcatgtggt
     5821 gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg cagggcaaga
     5881 gccaaaccgc gcattggcgc agccgctgat tgatagcggg aaaacggtgc atttaattgg
     5941 cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg gaacacggct
     6001 ggcgctggag atttaaatcg tgcattg
//
LOCUS       DAZFDC010000199         5986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFDC010000199 DAZFDC010000000
VERSION     DAZFDC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.199"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(150..911)
                     /gene="kduD"
                     /locus_tag="ACHJ48_004490"
     CDS             complement(150..911)
                     /gene="kduD"
                     /locus_tag="ACHJ48_004490"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO4709901.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(941..1777)
                     /gene="kduI"
                     /locus_tag="ACHJ48_004491"
     CDS             complement(941..1777)
                     /gene="kduI"
                     /locus_tag="ACHJ48_004491"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO4709902.1"
                     /translation="MDVRQSIHSAHAKTLETQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIIGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2064..3245)
                     /gene="yqeF"
                     /locus_tag="ACHJ48_004492"
     CDS             complement(2064..3245)
                     /gene="yqeF"
                     /locus_tag="ACHJ48_004492"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO4709903.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3500..4729
                     /gene="yqeG"
                     /locus_tag="ACHJ48_004493"
     CDS             3500..4729
                     /gene="yqeG"
                     /locus_tag="ACHJ48_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO4709904.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5189..5821
                     /gene="yqeH"
                     /locus_tag="ACHJ48_004494"
     CDS             5189..5821
                     /gene="yqeH"
                     /locus_tag="ACHJ48_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4709905.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWTEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         1662 a         1427 c         1253 g         1644 t
ORIGIN      
        1 accacctaat tttaaagcag ataaaattca taaagttcat taattgataa ttgatatgga
       61 ttatttcata accatgatat ggattatgat gatctagaga tataaaaaac cctgccatgc
      121 ggcagggtca taaaagtaag aggaatgaat taacgcgcca gccaaccgcc atccacagca
      181 atggtataac catttacata atctgaagcg ctggaggcaa gaaacactac cggccccatc
      241 aggtcactcg gcagtcccca acggccagct ggaatgcggt cgagaatttc cgcgctacgt
      301 tgttcatctg cccgcagttg ttgggtattg ttggtcgcca tgtaacccgg agctatcgca
      361 ttaacattaa tgttgtgttt agcccattca ttcgccatca atcgcgtcac gcccatcacg
      421 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
      481 gcgatattga taatcttgcc gccattgcct tgcgcgatga agtgtttcgc cgctgcctga
      541 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc cttttcgctg
      601 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
      661 ccaaactcag ctaccgcgcg atccagcagt ccaggaatac catcaatctt tcgcagatcg
      721 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt
      781 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
      841 tggcccagcc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
      901 cttaaaatca taacaatacc ttattcgttt atgcccacaa ctagcgcaaa tctttaacgg
      961 ccacatggtc catatcatca aagacctggt tttcaccgac catgccccag ataaaggtat
     1021 aagctttggt tccgacgccg gaatgaatcg accagctcgg ggagatcacc gcctgctcgt
     1081 tatgcatcac aatgtgacgc gtttcttgcg gctgccccat catgtggaaa acgcaggcgt
     1141 catcatccat attgaaatag aaataaactt ccatccggcg ctcgtgggtg tgacacggca
     1201 tggtgttcca caagttaccc ggagccagct ccgtcagccc catactcaat tggcaggttt
     1261 ccagcacatc cgggacaaaa tatttgttaa tcgtgcgacg gttactggtg aggttatcgc
     1321 ctaacgtgac tggagatact tcgtccggtg tgactttttt ggtaggatac gtcgtatgcg
     1381 cgggtgcgca attgtaataa aacttcgccg gagtgccggt atcaatacta gcaaagacaa
     1441 cttcttttgc gcctttaccc acatacaggg cgtcgcggtg accgatttca tagcattgac
     1501 catcgacagt aatcgtaccg gcaccgccaa tattgataac acctaactcg cgacgttcaa
     1561 ggaaataact tacgccgagt tgtttaccca cttccccgcc aacggaaacc gttttagtta
     1621 ccggcataat gccgccaata ataatacggt caatgtggct gtaaaccatg gtgtactcat
     1681 cggcgacaaa taccttttca accaaaaatt cattgcgcag cccctgggtt tccagcgttt
     1741 ttgcgtgcgc actgtggatg ctctgtctta cgtccacatc aacctccgat aatcagtgaa
     1801 gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt tctctataag gggatgatat
     1861 gcccatggga tcgggttttc aattaaaaat aaaacaatgt ttcattttta agttaaggat
     1921 taaaaaaagt gcagtgtcga tcacgaatct gtcatgagaa tggaaaaatc agggagcccg
     1981 gaaaatgtat gtcgacttgc cagccatcat tattcaacag attaatgacg gcagatattt
     2041 atcggtaagg aggaaatgca gagctattcg tcacgttcaa tggtcaatgc cacaccctgg
     2101 cccccgccga tacaaagcgt tgccagtcct ttgcgggcat tacgtttcac catttcatga
     2161 accagagaaa ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg
     2221 ccaccattga cattgacccg acgctcatcc cactcaagca tcttgccaac cgaaagcgcc
     2281 tgtgcagcaa acgcttcatt agcctcgata agatcaacat cagccaactg ccagcctaca
     2341 cgctccaggc aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc cggatctaca
     2401 ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta aattcaacgc tcgtgctttg
     2461 gcttcgctca tcatcattac cgcagctgcg ccatcgttta tggatgatgc attacccgct
     2521 gtcacagaac cgagactatc aaatgaagga tttaaacggg ctaaaccttc tgcactggcg
     2581 tcagtgtgtg gctgttcatc ggtatcaaca accaacgtct gaccgttact ttgggtcatt
     2641 accgggacga tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc
     2701 gagctaagtg cgtaagcatc ctgcaattga cggctgatgc catattcgcg agccagattt
     2761 tcggcggtga caccaatatg ataatcattg aaggcatccc ataacccatc atgcacaaga
     2821 ctgtcaacca gctggctatt gccaagctgt gcaccggtgc ggctatcagt cagaacatgt
     2881 ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa tatcagcctc gccacactgt
     2941 atcgcctgag tagccagatg cagtgcttta agcccggaac cgcagacgtc attaatcgtt
     3001 attgcagaaa cgctattagg cagaccacct ttaatagccg attgccttgc cggattctgc
     3061 cctgcccctg cagtcaacac ctgaccaaga attacttcat ccaccgcata tgcaggaacg
     3121 ccggtacgtt ctattaacgc tttcacgacc agactaccaa gttccacggc ggaatgaccc
     3181 gctaacgcac cgcgaaagca gccgataggt gtccgtaacg ccccgacaat cacaacgtct
     3241 ttcatcacaa cctcgcacca attaacagtg caatagtaaa tgattgttaa ttacagttat
     3301 cttaattgtt taaaaaaagt gatttttatc acaaaggaaa tatacctgag cagtagtcag
     3361 agtcataact ggcacgtaag gtttgcaacc actaccccac caatagaggg gtagataggg
     3421 cgttaatctc ccatacttaa cctggtttat ggtaaattgc cctccatttt gtttaatttg
     3481 tagatgatac gttcagataa tgtctaatat ttggtctaaa gaagaaactc tgtggagttt
     3541 cgcgctctac ggcacagccg ttggtgcagg cacgctcttc cttcctattc agttaggttc
     3601 ggcaggggct gtggtcctgt ttattactgc tctggtcgcc tggcctttaa catactggcc
     3661 acataaagct ttatgccagt tcatcctctc atcgaaaaca tcagcaggtg aagggataac
     3721 gggcgcggta acacactact atggcaagaa gattggtaat ctgattacca cgctgtactt
     3781 catcgccttt tttgtcgtcg tgttgatata tgcagtggca attaccaact cacttacgga
     3841 acagctggca aagcatatgg ttattgatct tcgcatccgt atgttggtga gtctgggtgt
     3901 tgtattaatt ctgaatctca tttttctgat gggacgtcat gccactattc gggtaatggg
     3961 atttttggta ttcccattga ttgcctattt cttatttctt tccatttacc ttgtaggtag
     4021 ttggcaacct gatctattaa caacccaggt agagttcaat cagaataccc ttcaccagat
     4081 atggatatcg attcccgtga tggttttcgc ctttagccat acgcccatta tttctacgtt
     4141 tgccatagac agacgtgaaa aatatggcga acacgctatg gataaatgca aaaaaattat
     4201 gaaagtcgct tatctcatca tctgcataag tgtactgttc tttgtcttta gctgcctgct
     4261 ttctattcca ccttcgtata ttgaagcggc taaagaagaa ggggtcacca ttttatcggc
     4321 gctttctatg ctgccgaacg ccccagcatg gttgtcaatt tccgggatta ttgtcgcagt
     4381 agttgcgatg tcgaaatcat tcctgggtac gtactttggc gttattgaag gtgccacgga
     4441 ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa agtcgtgcat ttaaccgcgc
     4501 actatcaatt atgttggtat cgctgattac cttcattgtt tgttgcatta acccgaacgc
     4561 gatttcgatg atttatgcga tcagcggccc gctcattgcc atgatacttt tcatcatgcc
     4621 tacgctatca acatatctca tcccggcgct taaaccctgg cgctccatcg gaaatctgat
     4681 tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc tttagctaat atccattgtg
     4741 aatggttcaa aattgctaca attttgaacc attccagttt tcataatgtg catttgttat
     4801 tattccgctc tatcacttct ctcgtcatat cctagtatta tcagttagtt aaacacacaa
     4861 agagatctga acatcttaaa taagactatt taagatgcat aacttagatt cgcaagatat
     4921 cttctggtca ttcaaaaaca atttgcgata tagatattct cataccatga gatagttatc
     4981 tgaaccaccc tcaaccaggt aagattaaat cagtgaaaca taatcatatt taataacgag
     5041 aaatgcattt taaaatgtct taatcagaat tatagcaaat acagattaat cgcaattttt
     5101 cccagcaaaa ttacaaaatc atggtgtatt gacatcatca atcaccaatg aaattatgca
     5161 attatatacg gatagggagg ttcttaacat ggggctttgt agtcgttata aaagtcttac
     5221 atgcaatagt tgctcaatgc attgccaaat aatgccagaa gagtcaccgc gtttgcagta
     5281 ctgcgcaaac tcgtgttttt gtatgtggac cgaagaaagt tcatatttta atcgtggtgt
     5341 ggtagaaggt atcttaacaa aaaaccacaa tgcgagatta agcggatata tttttgtcga
     5401 tttttcagta agttttttac gtctattcct tgaaaaagac tggattgact atcttgcaag
     5461 tactgacatg gggattgttt tagtcagcga ccgcaatatg caatcgttag ctaattactg
     5521 gcgaaaacac aactctgcaa tatctgctgt catatataat gatgatggtc ttgatgttgc
     5581 caatgaaaag atcagacaac tatttattgg tcgttattta tcatttacca gaggtaacac
     5641 attaactcag atggaattca ccataatggg gtatatggtt tctggttata atccatatca
     5701 gattgctgaa gttctggata tggatatccg tagcatctac gcgtacaagc aacgaatcga
     5761 aaagagaatg ggtggtaaaa taaacgaatt atttattcgt tcacattcgg tccaacattg
     5821 atactcaaca atctgccaga atcatactct gtttatacgg gcatttattg aaatgcccgt
     5881 attttataaa taggccgtcc cctcaagagt aaacaccata gtatttaaaa ccatcacacg
     5941 aaaaattcag aagcattacg aaatatgaac ttacaaacaa tagtgg
//
LOCUS       DAZFDC010000200         5956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFDC010000200 DAZFDC010000000
VERSION     DAZFDC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.200"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            112..630
                     /locus_tag="ACHJ48_004495"
     CDS             112..630
                     /locus_tag="ACHJ48_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO4709906.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            840..2981
                     /locus_tag="ACHJ48_004496"
     CDS             840..2981
                     /locus_tag="ACHJ48_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4709907.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIASILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVLYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQILNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3057..>5956
                     /locus_tag="ACHJ48_004497"
     CDS             3057..>5956
                     /locus_tag="ACHJ48_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4709908.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWK
                     APFDIRLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPL
                     SALWQVLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVV
                     DGFGRTLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPA
                     GPRSASSSLVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDELPAAPLARYTYTASG
                     ELRAVYDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTELVNPEGLDY
                     RFEYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTD
                     AAGRRTEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGR
                     LAAETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLTFTDCSGYTTRY
                     EYDRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTAIVAPDG
                     SRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQR
                     GFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDEHGWL
                     TTLSHTSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQ
                     EPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYMRDRLHRETARSFGGEAYELATAWNTS
                     GQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDID
                     IPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYIYRYDEYGRLAEKTDR"
BASE COUNT         1353 a         1592 c         1931 g         1080 t
ORIGIN      
        1 aaaaatactg aaaaatgatg cggttccgtg aagtgagctg catctgcctg tctgggatta
       61 acggcaggtc attgtaagta gtaattaacc acgttaacaa ggagtttaca aatgccaaca
      121 ccgtgttata tctccatcac tggtcagacc cagggaaata ttactgcagg tgcatttacc
      181 gctgattctg tcggcaacat ctatgtgcag gggcacgaag acgagatgct ggtgcaggaa
      241 ttcctgcata acgttactgt cccgaccgat cctcagtccg gtcagcctgc gggtcagcgt
      301 gctcacaagc ctttcatttt taccgtggcg ctgaacaaag cggttccgct gatgtacaac
      361 gcactggcct ccggcgaaat gctgccaacc actgaacttc actggtggcg tacctctgtg
      421 gaaggcaaac aggagcatta tttcactacc cgcctgactg attcgacgat tgtcgatatg
      481 aaactgcata tgccgcattg ccaggacccg gcgaaacgcg agttcaccca gttgctcgaa
      541 gtttcactgg cctaccgcaa aattgaatgg gaacacgtca aatctggcac ctccggtgcc
      601 gacgactggc gcgcaccgct ggaagcataa gttaagccaa cagcatccgg ctatcaggcc
      661 ggatgctgtt tttgtattac gccgtagaaa gaaaggttgt cagtaagggg ttacgttcac
      721 gggagatggc ggctgccgga aacttcggcg gtagcgtgcc tgaacgcaag cgtagcactg
      781 tactgtcaca gccgctttaa cagcgaactg tctgtttttc cttaaccccg gaggttgcta
      841 tgtcaaccgg attacgtttc acgctggaag tggacggcct gccaccggat gcttttgcgg
      901 tggtctcctt tcatctgaac cagtcactct cttcgctttt ttccctcgct ctctctctgg
      961 tcagccagca gtttctctcc cttgaattcc agcagatcct cgacaaaatg gcctacctga
     1021 cgatatggca gggcgatgac gtacagcgcc gggtgaaagg tgtggtgacc tggtttgaac
     1081 tgggggagaa cgacaaaaac cagaagctgt acagcatgaa ggtatgcccg ccgctgtggc
     1141 gcacagggct gcgccagaac ttccgtatct tccagaatga ggacatcgca agcatcctcg
     1201 gcacgatatt gcaggaaaac ggggtgaccg agtggagccc gctgttcagc gagccacatc
     1261 cttcccgtga gttttgtgtc cagtacggtg agactgatta cgatttcctg tgccggatgg
     1321 cggcggagga aggcatcttc ttttatgagg agcacgcgca aaaaagtacc gaccagagcc
     1381 tggtcctgtg cgataccgtg ctttatctgc cggagtcctt tgagatcccc tggaatccga
     1441 acacccgtac cgaggtaagc accctctgca tcagccagtt tcgctacagc gcacaaatcc
     1501 gcccttcttc cgtggtgacc aaagactaca cctttaaacg ccccggctgg gccggacgtt
     1561 ttgaccagga aggccagcac caggattacc agcgcacaca gtatgaagtg tatgactatc
     1621 ccggacgttt caagggcgcc cacgggcaga actttgcccg ctggcagatg gacggctggc
     1681 gaaacaacgc agaagtggcg cgcggaacaa gccgttcgcc ggagatatgg ccgggacggc
     1741 gaattgtgct gacggggcat ccgcaggcga acctgaaccg ggaatggcag gtggtggcaa
     1801 gtgaactgca cggtgagcag ccgcaggcgg tgccgggacg caggggttca ggcaccacgc
     1861 tggataacca ttttgcggta ataccggcag acagaacatg gcgaccacag ccgttgctga
     1921 aaccgctggt ggacggcccg cagagcgccg tcgtgacggg accggcaggc gaggaaatct
     1981 tctgtgatga acatggccgc gtgcgggtga aatttaactg ggaccgctat aacccgtcaa
     2041 accaggacag ttcatgctgg atccgtgtgg cacaggcgtg ggcaggcacc gggtttggta
     2101 acctggcgat accgcgtgtg ggtcaggagg tcattgtgga cttcctcaac ggcgatccgg
     2161 accagccgat cattatgggg cgtacctacc accaggaaaa ccgcacaccc ggtagcctgc
     2221 cggggacgaa gacgcagatg accattcgtt cgaaaaccta taagggcagc gggtttaatg
     2281 aactgaagtt tgacgatgcg acagggaaag aacaggtcta catccacgcg cagaagaaca
     2341 tgaacaccga ggtgctgaat aaccgcacca cggatgtgat aaacaaccat gccgaaaaaa
     2401 taggtaacaa ccaggcgatc accgttacca ataaccagat cctgaacatt ggcgttaatc
     2461 agatacagac ggttggtgtc aaccaggtgg aaacggtagg cagtaaccag attatcaaag
     2521 tgggatcaaa ccaggttgaa aaggtgggga tcattcgtgc gctgacggtg ggtgtggcgt
     2581 atcagacgac ggtaggcggc attatgaata cctcggtggc gttgttgcag tcctcacagg
     2641 tagggctgca taaatcactg atggtgggaa tgggctacag cgtcaatgta gggaataacg
     2701 tcaccttctc ggtgggcaag acgatgaagg aaaacaccgg acaaacagca gtttattctg
     2761 ccggtgagca tcttgaactc tgctgtggta aggcaaggct ggtgctgacg aaggacggaa
     2821 gcatatttct taatggtacg cacattcatc tggaagggga gtcggatgtg aacggtgatg
     2881 cgccggtgat taactggaac tgtggtgcca cacaacctgt accggatgcg cctgtgccga
     2941 aagatttacc ccccggaatg ccggatatgc ggcaattttg agtcaggtgc aaaaaatcaa
     3001 aacggaactg gtgcagtgac cagataaacc atattaaaag cagaaggaat tgattcatgg
     3061 gaggaaaacc ggcggcgcgg cagggtgaca tgacccgcaa gggactggat attgtgcagg
     3121 gttcagcagg ggtgctgata ggtgcgccga cgggcgtggc ctgctcggtg tgtccgggag
     3181 ggattaccta tgctaacccg gtgaacccgg tgctgggtgc gaaggtgctg ggtgcgaagg
     3241 tgctgccggg cgagacggac cttgcgctgc ccggcccgct gccgtttatt ctttcccgcg
     3301 cctacagcag ctaccggacc agaacacccg cgccggtggg ggtgtttggt cccggctgga
     3361 aagcgccctt cgatatccgc ttacagatac gcgatgaagg cctgatactc aacgacaacg
     3421 gcggcaggag cattcacttt gagccgctgt ttcccggcga gataagctac agccgcagcg
     3481 agtcgttctg gctggcgcgg ggcggggtgg cggagcagca cagttcgcag ccgctaagcg
     3541 cgctctggca ggtgctgccg gaagatgttc gcctgagtcc gcatatgtac ctggcgacaa
     3601 acagccttca ggggccgtgg tggatactca actggccgga gcgggtgccg ggggcggacg
     3661 aggtgctgcc gccggagccg cccgcatacc gggtgctgac gggcgtggtg gatggcttcg
     3721 ggcggacgct ggcctttcac cgggcggcgg agggtgatgt ggcgggcgcg gtgacggggg
     3781 tgacggacgg cgcgggccgc cgttttcacc tggtgctgac cacacaggca cagcgggcgg
     3841 aagtattccg taaacagcgc gccacgtctt tatcttcccc tgccggtccc cgttctgctt
     3901 cctcttctct ggttttcccc gacacgctgc ccgccggtac agaatacggt gccgataacg
     3961 gtatccggct ggaggcggta tggctgacac acgacccggc atacccggat gaactgcccg
     4021 ccgcgccgct ggcgcgctac acgtacacgg ccagcggaga actgcgggcg gtgtatgacc
     4081 gcagcgggac gcaggtgcgc gggtttgctt atgatgcgga gcacgccggg cggatggtgg
     4141 cgcaccatta tgcgggtagg ccggagagcc gctaccggta tgatgatacc ggccgggtga
     4201 cggagctggt caacccggag gggctggact accgctttga gtacgggcag gaccgtgtga
     4261 ccatcacgga cagcctgaac cggcgggagg tgctgtacac ggaaggcgag ggtggcctga
     4321 aacgtgttgt gaagaaggaa catgcggacg ggagcatcac ccgcagcgag tatgatgagg
     4381 cggggaggct gaaggcacag acggatgcgg cgggacggcg gacggagtac agcctgcata
     4441 tggcgtcggg tgcggtgaca gcggtgacgg ggccggacgg caggacggtg cggtatggct
     4501 ataacagcca gcggcaggtg acgtcagtga cgtacccgga cgggctgcgc agcagccggg
     4561 agtatgatga gaagggaagg ctggcggcgg agacctcgcg cagcggagag acgacgcggt
     4621 acagctatga tgacccggcg agtgagctgc cgacagggat acaggacgcg acgggcagta
     4681 caaaacagat ggcatggagc cgttacggtc agctgctgac ctttacggac tgctcggggt
     4741 acacgacgcg gtatgagtat gaccggtacg gtcaacaaat cgccgttcac cgggaagaag
     4801 gcatcagcac ttacagcagt tataacccgc gtggccaact ggtcagtcag aaggatgcgc
     4861 aggggcgtga aacccgttat gagtacagcg ccgcaggcga cctcaccgct atcgttgccc
     4921 cggacggcag ccgcagtgag atacagtatg atgcgtgggg aaaggccgtc agcaccacgc
     4981 agggcggtct gacgcgcagc atggggtatg acgctgccgg gcgcatcacc gtgctgacca
     5041 acgagaacgg cagccagtcc acgttccggt atgacccggt ggacaggctg actgaacagc
     5101 gcggttttga tggccggacg caacgttacc actatgacct gaccggaaaa ctcacgcaga
     5161 gtgaagacga ggggcttgtc accctctggc actacgatgc gtcggaccgc atcacgcacc
     5221 ggacggtgaa cggcgacccg gcagagcagt ggcagtatga tgagcacggg tggctaacca
     5281 ccctcagcca taccagtgaa ggccaccggg tgtcggtcca ctacggctat gacgataaag
     5341 gccgcctgac gggtgaacgg cagacggtgg agaacccgga gacgggggag atgctgtggg
     5401 agcatgagac ggggcacgcg tacagcgaac aggggctggc gacccgtcag gagccggacg
     5461 gtctgccgcc ggtagagtgg ctgacgtatg gcagcggtta tcttgcgggg atgaagctgg
     5521 gcggaacgcc actggtcgag tacatgcggg accggctgca ccgtgagacg gcccgcagct
     5581 tcggcgggga ggcatatgaa cttgccaccg cctggaatac cagcggccag ctccggagca
     5641 ggcacctgaa ccttccgcag cttgaccgtg actacgactg gaacgacaac ggacagctaa
     5701 tccgcatcag cggcccgcag gagagccggg agtaccgtta cagtgacacg ggaaggctga
     5761 cgggcgtcca caccacggca gcgaacctgg atatcgatat cccgtatgca acggacccgg
     5821 caggaaaccg gctgccggac ccggaactgc atccggacag cacgctcacg gcgtggccgg
     5881 ataaccgcat cgcggaagat gcgcactata tctatcgcta cgatgaatac ggcaggctgg
     5941 cggagaagac ggaccg
//
LOCUS       DAZFDC010000201         5928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFDC010000201 DAZFDC010000000
VERSION     DAZFDC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.201"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            27..755
                     /gene="gadW"
                     /locus_tag="ACHJ48_004498"
     CDS             27..755
                     /gene="gadW"
                     /locus_tag="ACHJ48_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO4709909.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1118..4231)
                     /gene="mdtF"
                     /locus_tag="ACHJ48_004499"
     CDS             complement(1118..4231)
                     /gene="mdtF"
                     /locus_tag="ACHJ48_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO4709910.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVIYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4256..5365)
                     /gene="mdtE"
                     /locus_tag="ACHJ48_004500"
     CDS             complement(4256..5365)
                     /gene="mdtE"
                     /locus_tag="ACHJ48_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO4709911.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVKLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5473..5751
                     /locus_tag="ACHJ48_004501"
     CDS             5473..5751
                     /locus_tag="ACHJ48_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709912.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5752..>5928)
                     /gene="gadE"
                     /locus_tag="ACHJ48_004502"
     CDS             complement(5752..>5928)
                     /gene="gadE"
                     /locus_tag="ACHJ48_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO4709913.1"
                     /translation="HNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKLR
                     RMSDIVTLGITSYF"
BASE COUNT         1447 a         1485 c         1498 g         1498 t
ORIGIN      
        1 cgcagtatga aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc
       61 gttcattcga tatttatcat gaacagcaaa aaatatcgct gcataacgag agtatcctgc
      121 tgctggagaa aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg
      181 atatcgatga gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt
      241 tagggttaca tagcaaagac cgtctgttaa ttaaccagtc accccccatg ccgctggtga
      301 cggcgatttt tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc
      361 tctacctttc ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca
      421 atagcatcag cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac
      481 gttggtatct gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa
      541 agttgcagga tgaaaacacc tgtttcagta aaatattact cgcctccagg atgtcgatgg
      601 ccagacgatt actcgagtta cgtcaaattc ctctgcatac tattgcggaa aaatgtggct
      661 atagcagtac gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc
      721 agtttgcgca acattcgcca ggtacctttt cctgacatat tttgcatttg aatattggtc
      781 aggatctcac acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc
      841 tagttctctt tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca
      901 tgtttgggat tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc
      961 tgttcaaatt gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc
     1021 tggtattcct gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg
     1081 ctgaaccttc acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa
     1141 agagatgttc cactacaacg aaaaagaccg gaacaaagta aattgccagc actgttgcgg
     1201 caaacatccc gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat
     1261 gactgataac cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc
     1321 gtaaacgcat ccgcgccgct tcgatgattg cctctatcgg cgttttccct tctttctgca
     1381 tcatctcaac ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg
     1441 tcagcaaacc aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta
     1501 atgcgccaac gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct
     1561 cataaagtgc ggcgagggcg aggaacacca cgaccagtga aatcgcatac agcgcaggag
     1621 cctgatttga ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg
     1681 ccggaagttt agcgaccagg tctgccataa atttcatggc gtcaccggta cttttcccgg
     1741 ccgccgcttc acctaaaatc tccattgacg ggatgccgtt gtagcgttcc agtcgcggtg
     1801 aaccataggt ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt
     1861 tgcgtacata ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga
     1921 catacacttt tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg
     1981 cggtggaaat tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg
     2041 cgttgacgtt cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga
     2101 cctgattcgg tgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt
     2161 cgtgccccag gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt
     2221 cagccaccgc gggtaagttg aacgggaaga cgacggcttt attgatactg cttagcgcaa
     2281 tcattgcccg ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg
     2341 gcttgagact gataaacgcc agaccgttgt tttgcccctg gccgctgaag ccaaagccgc
     2401 caacggtaaa caccgactga acattatctt tctcttttgt aagataataa tccgtcacct
     2461 gttgcagcac tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca
     2521 taaatacccc ctgatcctct tctggtaaga aagaggtcgg cgtgcgcagg aacagcaccg
     2581 ccatcccggc gcaaatcagc aggtagatca ccatgtagcg accggtacaa cgcaacagtg
     2641 agcgggtgct atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga
     2701 acagggcatt aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg
     2761 caggggtcag gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg
     2821 agaactggcg gtagatctcc ccggttgcac cgctcataaa ggccatcggc ataaacactg
     2881 cggaaagaac aacggcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg
     2941 tcgcttcctt cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca
     3001 cgatggcgtc atccaccagt aaccctatcg ccagcaccat cccgaacata gtcaacgtgt
     3061 tgatggtgaa accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg
     3121 caatcgtcgg gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga
     3181 cgaggatgat agcctcaacc agtgttttga aaacttcctg aatagaaatt tcgataaacg
     3241 gcgtggtgtc gtaaggataa accgtcttca gacttgccgg gaaataggct gataagcggt
     3301 tcagttcctc tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga
     3361 tggcgatccc ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgccc
     3421 caagttcgac gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca
     3481 acaggatttt gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt
     3541 ttagttgctg gtctgccgcc tgtggcatgc cacccagttg accaccagaa atctggttgt
     3601 tttgcacctt aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt
     3661 gcgggtccag ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc
     3721 ccgcggtacg gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact
     3781 ggttgaggct gccgttatca gaaataaacg ccgctaccat caggatatta ctgctcgatt
     3841 tatcgacgct aatcccctgc tgctgcactg cttcaggtaa tgaaggcata gcgagttgca
     3901 gtttattttg cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag
     3961 tgatagaggc attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc
     4021 cattcatatt ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac
     4081 ccggataggt agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg
     4141 gtaagttcat gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa
     4201 aaaccgggcg atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt
     4261 gtttcgattc ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg
     4321 gacgaatgcg ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga
     4381 caacccactg gtcgccgatg gctttgctgg cttcaatttc gcgtagcttc acgacatcgt
     4441 ctttatccag aatgagcgcc gttgctttac cttgggcgtt gtgggtgacg ccttcctgcg
     4501 gcaccagtaa tacattctgg cggctacctt catccactaa tgccgtgaca tacatgccag
     4561 gcagcaagtc accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt
     4621 catcaaccgt cgggtcggag aatttcagcg tgccggtctg gctgtaacgt ttaccatttt
     4681 ccagattgag ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct
     4741 cttctttcat gcgtaagaaa tcttgcaccg actgcgtgag atcgacataa atcgggtcca
     4801 gacgttgtac ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca
     4861 ccgacgattt cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga
     4921 tcgtcgcctg ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcaact
     4981 gggtgcgcgc ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct
     5041 ggcggttaaa ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt
     5101 tggcggagtt tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct
     5161 ggttcacttt atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac
     5221 gtatctcggc aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg
     5281 acccagggga gagtgtgacg acaccgacct caggcgtcat ggcggcggtg ttttccgccg
     5341 atttgtcatc gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc
     5401 ttcttctgtt cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc
     5461 gcgaggagag atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag
     5521 gtgggataca ggcatagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt
     5581 ctgccaacag ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac
     5641 caggggaata aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc
     5701 atctgcatcc ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata
     5761 agatgtgata cccagggtga cgatgtcgct catacgtctt agtttcagct tgtacataat
     5821 gttgaatttc tgaatcttta atgtttgttg cgaaatattg agcgtcgacg tgatattgct
     5881 ttcattttta tttttcagaa tcagttgcat aattttgaac tgattatg
//
LOCUS       DAZFDC010000202         5883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFDC010000202 DAZFDC010000000
VERSION     DAZFDC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.202"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..95
                     /locus_tag="ACHJ48_004503"
     CDS             <1..95
                     /locus_tag="ACHJ48_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFO4709914.1"
                     /translation="VCKNVSRLAGELSIVRVLNDFQPLPILRVY"
     gene            complement(92..340)
                     /locus_tag="ACHJ48_004504"
     CDS             complement(92..340)
                     /locus_tag="ACHJ48_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4709915.1"
                     /translation="MRVEICIAKEKISKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(566..661)
                     /locus_tag="ACHJ48_004505"
                     /pseudo
     CDS             complement(566..661)
                     /locus_tag="ACHJ48_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(658..1224)
                     /gene="yecD"
                     /locus_tag="ACHJ48_004506"
     CDS             complement(658..1224)
                     /gene="yecD"
                     /locus_tag="ACHJ48_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO4709916.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            1534..3306
                     /gene="aspS"
                     /locus_tag="ACHJ48_004507"
     CDS             1534..3306
                     /gene="aspS"
                     /locus_tag="ACHJ48_004507"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO4709917.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLRSVEFAVFAGPANDPKGRVAALCVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPAALAELSIQVVKKAENN"
     gene            3424..3876
                     /gene="nudB"
                     /locus_tag="ACHJ48_004508"
     CDS             3424..3876
                     /gene="nudB"
                     /locus_tag="ACHJ48_004508"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO4709918.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3905..4645
                     /gene="yebC"
                     /locus_tag="ACHJ48_004509"
     CDS             3905..4645
                     /gene="yebC"
                     /locus_tag="ACHJ48_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4709919.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4680..5201
                     /gene="ruvC"
                     /locus_tag="ACHJ48_004510"
     CDS             4680..5201
                     /gene="ruvC"
                     /locus_tag="ACHJ48_004510"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO4709920.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(5203..5805)
                     /gene="yebB"
                     /locus_tag="ACHJ48_004511"
     CDS             complement(5203..5805)
                     /gene="yebB"
                     /locus_tag="ACHJ48_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO4709921.1"
                     /translation="MNINYPAEYEIGDKVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1476 a         1428 c         1556 g         1423 t
ORIGIN      
        1 tcgtatgtaa gaacgtgtcg cggttggctg gtgaactttc gatagtgcga gtattgaatg
       61 atttccagcc gttaccgatt ttacgtgttt attagtgaac aaaccactcg tcagcagact
      121 cccaggtatc tttcagagtc tcctgaacaa atgtttttgc agaatcttta tctgcggtgc
      181 gtgtaacaga aagcccatcg ttgctggtgg cttttacgat cacctctaca tcgtcataac
      241 gtttactgat gcgtcgggtt aattcttcct ttaacgcatc cacagcaccg tttggcattt
      301 tgctgatttt ttctttagca atacagatct caacacgcat aacaccctcc tgtaactgtg
      361 gttatataca ggtgttattt ttatctgtat ggataaccag tgtcaattcc tgatattgtt
      421 tatggggcat caatggggca tgtatgggac actttttatc ggcgaaattc gtcgaagttc
      481 gtcgacatgg taaacgaatc atctatccaa cccttgaaaa acggcgctcc tggacgatct
      541 tcgtcgattt ttaaaaatgt tgcgtcatgc agttaaagtg acgggcatac tcttcaaggc
      601 tggtgatccc caaccgcacc catttaggat gcgaccattg cggtagaccg atgtaaatca
      661 taacgcgttg aggatctctt ccacgctacg cacacgggcg atgcgcgggt agatatggtt
      721 gatactgtta ttgtgctgct cggcgctagc ggcactacag gcatcttcgg caatcaccag
      781 attaaagcca agttcccagg cattgcgggc ggtggattca actccgatat tggtcgagat
      841 cccacataac actattgtat cgatacctcg gcggcgtaat tgcaactcca gatccgtacc
      901 gtaaaacgca ccccactgac gtttgattat ttcgatatca ctgtcagttg cacctaatgc
      961 agctggatgt tgccaccagt tttcgggcaa cactttagcg ggggaagggg catcaaccgg
     1021 ctgttttaat gcttcggcgt aatcggcaga ccagccaacg cgcaccagaa acacgggctg
     1081 accgctggcg cgaaatttcg ccgccagctt cccggcgcga ttaactacct catcggcagt
     1141 atgtggacca ccggcaaaag gtaagatgcc ttcttgtaaa tcgatcacca ccagcgcagt
     1201 ggttttagca ttaagttcaa gcatgatgac tcctgaaaaa acaaaatcgt gcctcacacc
     1261 ttaatgcgat tagccgatgg cggaacgaca aattttgtta atttgtatga gttcgggcaa
     1321 cacctttata gcaaactggc gtagagtctg atttcaggcc cgatataaag tggtaacgag
     1381 gcgttccagc gcgggccagg ctcttacggt gcggcagaat ttccagtata atagccgcct
     1441 tttttcattc agttgtgaca tacagctaac gctgcgactt ggtcacctgc gcagcagggg
     1501 gcatccgccg gcagctaagt taagggatat ctcatgcgta cagaatattg tggacagctc
     1561 cgtttgtccc acgtggggca gcaggtgact ctgtgtggtt gggtcaaccg tcgtcgtgat
     1621 cttggtagcc tgatcttcat cgatatgcgc gaccgcgaag gtatcgtgca ggtatttttc
     1681 gatccggatc gtgcggacgc gttaaagctg gcctctgaac tgcgtaatga gttctgcatt
     1741 caggtcacgg gcaccgtacg tgcgcgtgac gaaaaaaata ttaaccgcga tatggcgacc
     1801 ggcgaaatcg aagtgctggc gtcctcgctg actatcatca accgcgcaga tgttctgccg
     1861 cttgactcta accacgtcaa caccgaagaa gcgcgtctga aataccgcta cctcgacctg
     1921 cgtcgtccgg aaatggctca gcgcctgaaa acccgcgcta aaatcaccag cctggtgcgc
     1981 cgttttatgg atgaccacgg tttcctcgac atcgaaactc cgatgctgac caaagccacg
     2041 ccggaaggcg cgcgtgacta cctggtgcct tctcgtgtgc acaaaggtaa attctacgca
     2101 ctgccgcaat ccccacagtt gttcaaacag ctgctgatga tgtccggctt tgaccgctac
     2161 tatcagatcg ttaaatgctt ccgtgacgaa gacctgcgcg ctgatcgtca gcctgaattt
     2221 actcagatcg atgtggaaac ttctttcatg accgcgccgc aagtgcgtga agtgatggaa
     2281 gcgctggtgc gccatctgtg gctggaagtg aagggcgtgg atctgggcga tttcccagta
     2341 atgacctttg cggaagccga acgccgttat ggttctgata aaccggatct gcgtaacccg
     2401 atggaactga ccgacgttgc tgatctgctg agatctgttg agtttgcagt atttgcaggt
     2461 ccggcgaacg atccgaaagg tcgcgttgcc gctctgtgtg ttccgggtgg cgcatcgctg
     2521 acccgtaagc agatcgacga atacggtaac ttcgttaaaa tctacggcgc gaaaggtctg
     2581 gcttacatca aagttaacga acgcgcgaaa ggtctggaag gtatcaacag cccggtagcg
     2641 aagttcctta atgcagaaat catcgaagcc atcctggatc gtactgccgc gcaagatggc
     2701 gatatgattt tcttcggtgc cgacaacaag aaaattgttg ccgacgcgat gggcgcactg
     2761 cgcctgaaag tgggtaaaga ccttggtctg accgacgaaa gcaaatgggc accgctgtgg
     2821 gttatcgact tcccgatgtt tgaagacgac ggtgaaggcg gcctgacggc aatgcaccat
     2881 ccgttcacct caccgaaaga catgacggct gcagagctga aagctgcacc ggaaaatgcg
     2941 gtggcgaacg cttacgatat ggtcatcaat ggttacgaag tgggcggtgg ttcagtacgt
     3001 atccataatg gtgatatgca gcagacggtg tttggtattc tgggtatcaa cgaagaggaa
     3061 cagcgcgaga aattcggctt cctgctcgac gctctgaaat acggtactcc gccgcacgca
     3121 ggtctggcat tcggtcttga ccgtctgacc atgctgctga ccggcaccga caatatccgt
     3181 gacgttatcg ccttcccgaa aaccacggcg gcagcgtgtc tgatgactga agcaccgagc
     3241 tttgctaacc cggctgcact ggctgagctg agcattcagg ttgtgaagaa ggctgagaat
     3301 aactgatatg actcaaatac acgaaatcat tcgcgttgca tcgaggcggc aactgagtga
     3361 actcccatga gcatagataa ctatgtgaat gggatgagcg aaggcagtca acgaagaggc
     3421 agcgtgaagg ataaagtgta taagcgtccc gtttcgatct tagtggtcat ctacgcacaa
     3481 gatacgaaac gggtgctgat gttgcagcgg cgtgatgacc ccgatttctg gcagtcggta
     3541 accggcagcg tggaagaggg tgaaaccgcg ccgcaagctg ccatgcgcga agtaaaggaa
     3601 gaggtcacca ttgatgttgt cgctgaacaa ctgaccttaa ttgactgtca gcgcacggta
     3661 gagtttgaaa ttttttcaca tttacgtcat cgctatgcgc cgggcgtgac gcgtaatacg
     3721 gaatcatggt tctgtcttgc gcttccgcat gagcggcaga tcgttttcac tgaacatctg
     3781 gcttacaagt ggcttgatgc gcctgctgcg gcggcgctca ctaagtcctg gagcaaccgg
     3841 caggcgattg aacagtttgt aattaacgct gcctgaacag gcagggctat ctggagatat
     3901 ttttatggca ggtcatagta aatgggccaa caccagacat cgtaaagctg cgcaggatgc
     3961 taagcgcggt aaaatcttca ctaaaatcat tcgtgagctg gtaaccgcgg ctaagctggg
     4021 cggtggcgat ccggacgcta acccgcgtct gcgtgcagca attgataaag cactgtctaa
     4081 caacatgacc cgtgacactc tgaaccgcgc aattgcacgt ggtgtgggcg gtgatgatga
     4141 tgcgaacatg gaaaccatca tctacgaagg ttacggtcct ggcggcacgg caatcatgat
     4201 tgaatgtctg tctgacaacc gcaaccgtac cgttgctgaa gtgcgtcatg cattcagcaa
     4261 atgtggcggt aacctcggta ctgatggttc cgtagcctat ctgttcagca aaaaaggcgt
     4321 catctccttc gagaaaggcg atgaagacac catcatggaa gcagcactgg aagcaggtgc
     4381 tgaagacgtt gtgacctatg atgacggcgc gattgatgtc tacactgcat gggaagaaat
     4441 gggtaaagtg cgcgacgctc tggaagcggc aggtctgaaa gcagacagcg cggaagtttc
     4501 catgatcccg tctaccaaag ctgatatgga tgcagaaacc gcaccgaaac tgatgcgtct
     4561 gatcgatatg ctggaagatt gcgacgacgt gcaggaagtt taccataacg gtgaaatctc
     4621 tgatgaggtc gcagcgactc tctgatgagg cctgctaaac agcaaaacgg agacgcgtga
     4681 tggctattat tctcggcatt gatccgggtt cgcgcgtgac cggctacggc gtcatccgcc
     4741 aggtaggtag gcaactgtcc tacctgggta gcggatgcat acgcaccaaa gtggatgatt
     4801 taccgtctcg tctgaaactc atctatgcgg gcgtgacgga aatcatcacc cagttccagc
     4861 ccgattattt cgccattgaa caagtcttta tggcaaagaa cgctgactca gccctgaaac
     4921 tgggccaggc gcgcggcgtg gcgattgtgg cggcggtgaa tcaggagttg ccagtatttg
     4981 aatacgcggc acgtcaggta aagcaaacgg tggtaggtat tggtagtgcc gaaaaaagcc
     5041 aggtgcagca tatggtccgc accttgctga aactgcccgc taatccacag gcggatgccg
     5101 ccgatgcgct ggcgattgcc atcacccact gccacgttag tcagaatgcg atgcagatga
     5161 gcgaatcgcg gctgaacctg gcgagagggc gactgcgtta agttataccg cctcggtcag
     5221 ttccggctga ggcgtttcca ctccctccgc atgaatcaac accaaacctg gatgatgcca
     5281 caaactggct ggcgtgacgg ttttacgctc ccacggaata gaacccagga accagaattt
     5341 ccagaaagtg agttttgcat tcggattgct atttaacaat tctccaaacg tctctatttc
     5401 acccaccgga atacatagcg cttctttata aatatcaaaa acaaattttg aacagaactg
     5461 gcgcgaagat tcgtatttaa aaccggtgtg gtaaagtttg cgtagccggg agggaacctg
     5521 ttcaacaatt cgttgttttt gttgttctgt cagcccagcg tctaatcgct ttatagcata
     5581 gcgttgatta gacgaacgtt taataaaacg agatagcgtg gtgatggttg aaaggggaac
     5641 ccggctttct gcaaccagga agtcttcacc gttatgaccg ataatgatcc cgacgtgatt
     5701 actccagcaa tttgatgcag ctgatatttg accaaataag gcagcaccta tacatgtaaa
     5761 gactttatct ccaatttcat attcagcagg gtaattaata ttcacgttgt cattccattg
     5821 aaatagatac gaatatacgt aatagcataa aaaatttatt ttttcaggag gcgcaatgta
     5881 tat
//
LOCUS       DAZFDC010000203         5802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFDC010000203 DAZFDC010000000
VERSION     DAZFDC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.203"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..642)
                     /locus_tag="ACHJ48_004512"
                     /pseudo
     CDS             complement(1..642)
                     /locus_tag="ACHJ48_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
     gene            630..1046
                     /locus_tag="ACHJ48_004513"
                     /pseudo
     CDS             630..1046
                     /locus_tag="ACHJ48_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            1174..1710
                     /gene="yfdR"
                     /locus_tag="ACHJ48_004514"
     CDS             1174..1710
                     /gene="yfdR"
                     /locus_tag="ACHJ48_004514"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO4709922.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            1701..2051
                     /locus_tag="ACHJ48_004515"
     CDS             1701..2051
                     /locus_tag="ACHJ48_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFO4709923.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            2048..2521
                     /locus_tag="ACHJ48_004516"
                     /pseudo
     CDS             2048..2521
                     /locus_tag="ACHJ48_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            2668..2865
                     /locus_tag="ACHJ48_004517"
     CDS             2668..2865
                     /locus_tag="ACHJ48_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709924.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEVLMILCRSAMLSQN"
     gene            2823..3173
                     /locus_tag="ACHJ48_004518"
                     /pseudo
     CDS             2823..3173
                     /locus_tag="ACHJ48_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            3210..3491
                     /locus_tag="ACHJ48_004519"
     CDS             3210..3491
                     /locus_tag="ACHJ48_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFO4709925.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(3539..3712)
                     /locus_tag="ACHJ48_004520"
     CDS             complement(3539..3712)
                     /locus_tag="ACHJ48_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709926.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(3909..4442)
                     /locus_tag="ACHJ48_004521"
     CDS             complement(3909..4442)
                     /locus_tag="ACHJ48_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709927.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            complement(4589..4693)
                     /locus_tag="ACHJ48_004522"
                     /pseudo
     CDS             complement(4589..4693)
                     /locus_tag="ACHJ48_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(5055..>5802)
                     /locus_tag="ACHJ48_004523"
     CDS             complement(5055..>5802)
                     /locus_tag="ACHJ48_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO4709928.1"
                     /translation="KNVTLIKQINDLKMCVNEYIGDCTDEELNDREFIASVVDRAIFH
                     FAINSICNPEDNKDATLIERCTFDVETKNGLPSTVQLFYEESKDNEPLANIHLQAIGS
                     GFLTFVNACQEHDDNSLKLFASLLISLSYSSVYSDFAGRVNINEYNDNYLKAQFEELS
                     QRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDILSKMRDAAPKLIDFAK
                     SFDPNSKEKIKIYTGTSKLIYDLFGVKSEK"
BASE COUNT         1692 a         1301 c         1236 g         1573 t
ORIGIN      
        1 atttaccagc catgctgaca ccgaaaactc cagcgtcatg tgaccgtgac cggcaggcat
       61 atcaataacg ccactgtaaa tcagcgtatt atccagcgcg gtacggttat aaatttcagc
      121 accgtttttc cgcactatca gacggcatga ggagtaaata tcagtatgct ctctctcatg
      181 cttagcgcca ctgaatgcca ccgccggaat aacaatctgc cggtcaaacg gctgatcgtc
      241 ataaaccctg acggtaatgg tccctgatgg ccaccgttcc ggtgcacggg agtcccgggg
      301 gaaagctttg cccactgttt taacgagatc gccttcaatc tggttcgcgg acagttttcc
      361 cagaacccga cagttctcgt taatcgtgac gttgttgagc gtcccggagt tcgcattcac
      421 gttaccactg atatccgcat ttttagcagt cagctttcca tccggtgtca gggaaaatgc
      481 cggtggattt ccaccactgg taatggtggg ggccgtcagg cgcttcagga acacgtcgtt
      541 catgaatatc tggttgccct gcgccacaaa catcggcgtt tcattcccgt ttgccgggtc
      601 aataaacgcg atacgattgg cggcaaccag aatggattga agactgggcc gactaccttg
      661 tgggctttga tgctaatggt gacaccattc aggcaacaaa agcggctgcg gcggtccgta
      721 aaatcacgat tgaagcaaac cagaccgctg attttgaaga taatgacttc agcggcaaac
      781 gctccctgat ggagtctgtc gaagcgaaga ccaaagacat tatgccagtg gcatttgaat
      841 ttaaatgcat tccgtttgaa ggtctgaaag aacgtccgtt taaattacgc ctcagcatta
      901 tcactggcga tcgtccggta ctggttctgc gcattattca gctggaggcg gtgcaggaag
      961 aaatggctaa cgaatttcgt gatctgcttg ttgagaaatt caaggacagc aaagtagaaa
     1021 cctttattgg tactttcacc gcctgatttc attactgcaa atgcccctga gggggcattt
     1081 atggaaacgt aatttactca ataatcgccg gatggtgagg gattcttttt accagaattc
     1141 agcgcggtgc agcgcatata cgtggagaac aaaatgtcat ttattaaaac tttttccggg
     1201 aagcattttt attatgacag gataaataaa gacgacatcg atattaacga tatcgcggtt
     1261 tccctttcaa atatctgtcg ctttgccggt catctttcgc acttctacag cgtcgcccaa
     1321 catgcggttc tttgcagcca gctggtaccg caggaatttg cttttgaagc attaatgcat
     1381 gatgcaacag aagcgtattg ccaggacatc cccgctccac tgaaacgcct tcttcctgac
     1441 tataaacgga tggaagaaaa aattgacgcc gtaatccgtg agaaatacgg gttaccccca
     1501 gttatgagta cgcccgtgaa atatgccgat cttatcatgc tggcaaccga acgccgcgat
     1561 ctcgggcttg atgatggctc tttctggcct gtactggaag gcatcccggc aacagagatg
     1621 ttcaacgtga ttccactggc acctagccat gcctacggga tgtttatgga acgttttaac
     1681 gagttatcgg agttacacaa atgcgcatga atgttttcga aatggaaggg tttcttcgcg
     1741 ggaaatgtgt accgcgagat ctgaaagtga atgaaacaaa tgctgagtat ctggtgcgta
     1801 aattcgatga agtacgtgct gaggctcgca acgagggtat taactatacc gcaagccgtc
     1861 ttgctgctgc tttcaatcac ggatttatca ataagccttt ggctgaagtt ttcgacgtta
     1921 cacgcatgat tctgtcagca aaagaagagt tagctaatga atcgcatccg attgatggcc
     1981 tgtccggtga atatgcagag aaatcccttg aagaatgggc ggaacggctt cgcaaaggag
     2041 gcagccagtg actggacatg cagcaatcct cgacatgtgc tgtggcagtc gcatgttctg
     2101 gttcgataag aatgacgacc gggcgatatt tagcgatatc agaaaggaag agcacacatt
     2161 gtgtgatgga cgacgcctga ttatcagtcc tgatctgata gctgattttc gtgcactacc
     2221 atttgcagac gcatcttttt cgatggttat attcgaccct ccgcatcttg agcgtgttgg
     2281 tgataacgcc tggatgggaa agaaatatgg acggctgaat aaagatacct agcgtgatga
     2341 tttgcggcag ggatttaaag aagcctttcg tgtgttgcgt ccatccggcg ttctgatttt
     2401 taaatggaat gaaacgcaaa tacctgttcg ccagatattg gtactgaccg acagaaaacc
     2461 tgttatcggt caacgaacag gaaaaaacga taaaacccac tggattattt ttatgaaata
     2521 ggcatccagt gagtaggttc gtaaggttac agatacgtat atctgaataa ttaaattcag
     2581 ttctgtaaat aaaatttaat ccttaaccgg agggattcct gcaccctcag aacatcagga
     2641 gaccgcccga aagggcggta atgaaaaatg gctgaattaa ccaaatggct acaaaacacg
     2701 attaccggaa ttgaaacggt agtagacgat aaatcgtttg tatgtgatga aatagtattc
     2761 aaaatcgatg tggttaaaaa cgtacttacc gcatttaaag tcgcgctggt atcgctggaa
     2821 gtattgatga tactttgtcg atcagcgatg ctctctcaga actaaaccat ttcattaacc
     2881 ggcacgcaga caatacgaaa tatttaaaag tctggggtaa cggggccacc ttcgacaacg
     2941 taattttacg tggagcttac gagcgagcag gacaaatctg cccgtgggcg tactggaatg
     3001 accacgatgt acgcacgatc gttacgcttg ggcgttccat cggattcgac cccaaaatgg
     3061 acatgccttt cgatggcgaa cggcacaacg ccctggctga tgcccgtcat caggcaaaat
     3121 atgtttccgc tatctggcag aaattaattc ctgccaccag cacagaatta tgattttccc
     3181 gggtgcagcc ggttttgatg gagaaaatta tgaacacctt gtttttactg atggctgaat
     3241 tcaatacccc aaacattgaa ctctcagcag ttagccaaaa gtactttggt atgagtccag
     3301 ccacggcaga agcaaaagca aacgcttgta agttgcccgt tccaacatat cgcatcggca
     3361 catcacaaaa agcaaaacgt tgcatcaata ttcaagatct tgcggaatac atagacaaaa
     3421 ggcgagaaga aggacgtatc gagtgggaac aggtcagaac agtcaaacag aaggacaaag
     3481 aacatcacta aagaaaaaac ccgcctgaag gcgggttttc aaaaagcacc agctatgatc
     3541 atgctgcttt gcgacgacga agcttaccct gctgctcttt accagagaca gtagcgtgag
     3601 tgaacgcatt aggagcagcc ttcatcagaa cttcaacagc agcacccata cctgcgaatg
     3661 ctttcattgt gtcgaactta acctgtggct tggttgcttt ttgatctttc atagaaaact
     3721 cccgagacag taaaggcgtc tctaaccctt tctttaaagc tagcttgttt cgctaactta
     3781 tgccaatcga tcatgtcgat tggtgacatc gtttcttagt agtttaagca caaaacgact
     3841 gccatagatg tacctttaag gtaatctgga cgggtatcct acaatttgta gacccttctc
     3901 gtctatacct actgagcaaa tttaagaaag atatcctgca gctcatcaat gactgccgac
     3961 atcacataac cgcactgttc catgcggaaa ccaaaagact cgtaatactg caccagttct
     4021 ggtactggct ctacaatgtg gacaacttta cattcaacag ctttacaaaa tataaaagca
     4081 ctcataagag tgagtaaaac catgcgccct ttcaatgggt gagattcatc ttctctagaa
     4141 aacctttcga tcatatggat acgaaagatg ttttcttcaa ccccataaac acaaattgct
     4201 gctcctgatg gtattccctg aacccgacct tgctgaacaa gttttatgca gaactcatac
     4261 ttttctctgg agttgccata ggtacttaac gcatagtccc attcaagctc accatagcca
     4321 ccacacagaa tcttgtaatc atcatcactg agcggaccaa cagcaagagg taagccgaca
     4381 tgatcaataa tcaactggat attgttacgt actgattgac ctatctcgtc cagggtaagc
     4441 atcatggcct ctcaagcgga acactaaaag tcgcattata tctcattctt aagccgcgta
     4501 tggattacac cttgaaatga aaacgccggg ttcccaatag gctcccacaa agtatataac
     4561 tatttgtttt tcaaaaacgg tacatcctat cgagcattgg tgcaacgcta aaccgaccac
     4621 tccagtgaac gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag
     4681 aattaccgtg cattttcaaa tgtagagatt attttatcga tatatcatgt ggttatgtta
     4741 ttcagcatca ctgttcagga ggctcaataa cggggtacta taccataaca acaggaagcg
     4801 cctgtctcat tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga
     4861 atatctaatc ttattagatg ttagcgttgt gaaaagcaat gataaacaat acagattcga
     4921 cgataaataa aaatcacaca ttaaactccg gtgatatctc tccctgctaa tagcactgat
     4981 agagaaaaaa agaacccaat aaacattggg ttcttttatg taataccttt cataccatcg
     5041 aagaacttca catattattt ctccgattta accccgaata aatcataaat taatttagaa
     5101 gtgcctgtat atattttaat cttttccttt gagtttgggt caaacgactt agcaaagtca
     5161 attaatttcg gtgcagcatc tcgcattttg cttaaaatat caggggcgag cgttccttca
     5221 ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc cccatctgcc
     5281 agacgcttca tctctcccag gtacttcttc atatcacgtt gagataattc ttcaaactga
     5341 gctttcaggt agttatcatt atattcatta atattcactc ttcctgcaaa atctgagtag
     5401 acactagaat atgaaaggga aattaacagc gaagcaaata attttaagct gttgtcatca
     5461 tgttcctggc aggcattaac aaacgttaag aaaccagaac ctattgcttg caaatgtata
     5521 ttcgctaaag gttcattatc tttagattcc tcataaaata gctgaaccgt ggagggaaga
     5581 ccattcttgg tttctacatc aaaagtacat cgttctatga gtgtcgcatc tttattatcc
     5641 tcgggattac atatactatt aatcgcgaaa tgaaaaatag ccctgtcaac aactgatgca
     5701 ataaattcac gatcgtttaa ttcctcatca gtacaatctc caatatactc attgacacac
     5761 attttgagat cgtttatttg cttaattaac gtcacatttt tt
//
LOCUS       DAZFDC010000204         5761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFDC010000204 DAZFDC010000000
VERSION     DAZFDC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.204"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(256..864)
                     /locus_tag="ACHJ48_004524"
     CDS             complement(256..864)
                     /locus_tag="ACHJ48_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO4709929.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(1290..1358)
                     /locus_tag="ACHJ48_004525"
                     /pseudo
     CDS             complement(1290..1358)
                     /locus_tag="ACHJ48_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1617..1964
                     /locus_tag="ACHJ48_004526"
     CDS             1617..1964
                     /locus_tag="ACHJ48_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709930.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(2169..2870)
                     /locus_tag="ACHJ48_004527"
     CDS             complement(2169..2870)
                     /locus_tag="ACHJ48_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4709931.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2867..3796)
                     /locus_tag="ACHJ48_004528"
     CDS             complement(2867..3796)
                     /locus_tag="ACHJ48_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4709932.1"
                     /translation="MANTAEIFNFPVPDAAQKEPRVADLDDGYTRIANELLEAVMLAG
                     LTQHQLLVFLAVMRKTYGFNKKLDWVSNEQLSELTGILPHKCSAAKSVLVKRGIFIQS
                     GRNIGINNVVSEWSTLPESGKKNKVYLKEVNLPESGKKSLPKSGKGTYPNQVNTKDKL
                     TKDNIKPYSSENSGESSDLPENDLPVVKADAAIQNGSKWGTAEDLTAAEWMFDMVKTI
                     APSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWT
                     QLEINRNKQQAGVTAGKPKLDLTNTDWIYGVDL"
     gene            complement(3885..4424)
                     /locus_tag="ACHJ48_004529"
     CDS             complement(3885..4424)
                     /locus_tag="ACHJ48_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO4709933.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQIKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(4455..4677)
                     /locus_tag="ACHJ48_004530"
                     /pseudo
     CDS             complement(4455..4677)
                     /locus_tag="ACHJ48_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
     gene            4788..5480
                     /locus_tag="ACHJ48_004531"
     CDS             4788..5480
                     /locus_tag="ACHJ48_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO4709934.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVNHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
BASE COUNT         1525 a         1354 c         1274 g         1608 t
ORIGIN      
        1 atcaggtatg atgtaggaac tgaaaacaat attggatatc attttcggta tttctctgca
       61 aattccagcc cacaggatct cggtgtacaa tctgtcgctg atcgcgcaaa tttagggtca
      121 atcacttcat tttttgcatc aaaccctcac taacatcgaa cgattatagt acaagtagtg
      181 ttcttgactt ctcattaaac cgtcagcatt caattaatta caaaatacta gcggcatttc
      241 tctcccgcaa ctttattaca gccgttcaga acgtaaatgc gtcaatactc gactccactt
      301 atcatcctgc cacggccgga attttacatg tgccgtttct ctggcaagga ttgcctgtgc
      361 cttattgagt atccggggat attcttgttc gattgaagtg aagcgaccgg cttcgcggtg
      421 ttctgctacc tgaagaagcg gagtgacgtt ttggcaagcg gttaacattg tgtcacttgc
      481 ccgccataac caagcaagtg tgcaaagttc gttatcagtg aattgttttg tgattgggga
      541 ttgttgaatt tctcgatcaa gaatatccag cacccagcgg cggaattctt tggccacagg
      601 agtgcgggcg aacatggcga tcagatgggc gccacggagg gagaaaatgc gagtcttctt
      661 acgataattt cctgaggtcg ttgattcgac gacctgagac attccgtcag taaactcatc
      721 aatatttttg ttgtagatgt cggttactgc tttggtgctg gcatatttta atgctgccgc
      781 caattctttg ctggtaaacc agatctggtt gttgtgattt ataggagtga attttacatc
      841 gcggaatgcg agctgagtga ccatagcgat aatctcgttt tgaatttgag ctatcaccac
      901 cgacgacgcc aatcgactgg tggtggactg tgcaaggttg gcgtaaccgg tcaaaacgaa
      961 atccggcgct tccgaagaag ccctcacaca gcccaccata atttttgtgt agacgtgcac
     1021 tgcgcataaa aaaaccgcga acgcggttat gcgtcgtttt gatgtccggg acgccaatcc
     1081 cgtgtgccga ttttgcggca acgcacagaa tatagcgccg gataaataat gtcgtcaacc
     1141 cttgcatatg gggggcgcga tgatcactac taccaccata atcgctccaa ctgttgcaac
     1201 tactttaggc taagacatca cgatttccat atcaccttta gccgccagac aaacagagac
     1261 tccctgttct tggctgccaa aaacaccgaa tgcaaaaaat cgcaggtgta tttctgcatc
     1321 tgtggaggga gaagagagtt tggattgtat gtgcgcataa acgtccccgt ttagcgcaga
     1381 agtcaccgta gttgttcata ttctgatgac atgataattt cctattgaat agaaaaaatt
     1441 aacacgactc attcttatga ctggaaaaag caatcatgct aataatcatt gttgttccag
     1501 ttccaaatgc agcgaaataa tcaccaatat tttttctacc ggtttcgact ttgatgctat
     1561 attctgctaa cacctaacta acactacgtt tcacacagca attacatgga aataacatga
     1621 ctatcaaagc gactgacata gagattcttc atcgttatgc tgaaggggta atggaacgtt
     1681 ctaaccatca tgcaaaaaat gttggtgcag ccgctcttac attgctagga ggtgttatct
     1741 ggaaagccct gcccggatca atcgagataa gaacgtataa cggaagtctt gcaaatatgg
     1801 tgtggtggca aagcgaaaga acattaaaaa attatgcaat ctcatacaac cataattcat
     1861 gtgaaattga aatgagagat gaatcagtca aaggtgctgt attatttagc atttccaacg
     1921 aaaccactcc agaaaaaata ttatcacagc tttctgagct ttaattaaag gcggtgctaa
     1981 caccgcccat accattactc cacatcttac gcaacttgat aattatgctc tattaaatac
     2041 ccacaatatt tcatcaatgc atgggtcagc tacaaaatgt ttaccgtggt aattcctgtt
     2101 ttcgcaacat ctcaaaagaa caataaacac tgtatttctt caagtaacga agaatctttc
     2161 tttgcccgtc atacacttgc tcctttcagc ccaaacttag ctttgatttc tgcgatcttc
     2221 gccagagcct gtgcacgatt tagaggtcta ccgcccataa caggaagttg ttttactggt
     2281 tcaggtatcg tctcaccacg gttaattcgc gctgtcatac aggtcagttc atcggcagcc
     2341 ttgcgccgta attccgcgtc agtcagcgca ttggcccgca tgttctggta caggttggta
     2401 accaaccagt aatgcgcgtt cgatttccac ggataagact ctgcatccgg atacaggcca
     2461 cgcttccggc aatactcgta aaccatatca accagctcgc tgacgtttgg cagcccggcg
     2521 ttaacggatg cttcttcccg gcaccatgca acaaactgcc cgggtgatgg cagaaatggt
     2581 ctattctgcc gacgggcaac acgcattccg gcgctcacct gttccatcgt ggtgatcccg
     2641 ttttcccgga aagccagaac ccactggcgg cggatttcgt tcagttcgtt ctggtcacga
     2701 ttagccaggc tcgccgggaa agttgccagt aactggctga atacaccgtt gattatctgc
     2761 gctacctgct gtacctgcgg cttttcgtcg tactgttccg gcatgttgtt ggcgatccga
     2821 cgcatctgct cacggtcaaa gttaaccatc tgtgcggcga tgtttttcat aaatccaccc
     2881 cgtaaatcca gtcagtgttc gtcaggtcga gttttggttt gccggctgtc acgccagcct
     2941 gttgcttgtt tcggttgatt tcgagctggg tccacttgtc gcggagtttg gccggactca
     3001 gcacgttacc ggaccagaag ttgtcctggc aggcccagcg gaacagtaca cacatgtcgc
     3061 ggtggttacg tccatcacgt tcacgcatca gacggatatc gttagcccac cctgcaaaat
     3121 tcggttttct ggctgatggc gcgatggtct tcaccatgtc aaacatccac tctgcggcgg
     3181 tcaggtcttc tgctgtcccc cacttgctgc cgttctgaat cgcagcatcc gctttcacca
     3241 caggaaggtc gttttctggc aggtcagagg attcgccaga attctcggac gaataaggtt
     3301 ttatattgtc ttttgttagt ttgtcttttg tgtttacctg attcgggtaa gtgcctttac
     3361 ctgatttggg taaacttttc ttacctgatt caggtaaatt tacctctttc aggtaaactt
     3421 tatttttctt acctgattcg ggtaatgttg accattcact gaccacatta ttaatgccta
     3481 tattccgccc gctctgaata aaaatcccac gctttaccag aacacttttt gcagcagaac
     3541 acttgtgcgg caatatcccg gtcaactcgg aaagttgctc gttgctcacc caatccagtt
     3601 ttttattaaa gccatatgtt ttgcgcatga cagccaggaa gaccagaagc tggtgctgtg
     3661 ttaatccggc cagcattaca gcttccagca actcatttgc aatgcgcgta taaccatcat
     3721 cgagatctgc cacgcgcggc tccttttgtg ccgcatccgg cacaggaaaa ttgaatatct
     3781 cagcagtgtt tgccataatt cctcccgcaa tgagtgtgtt acgatttgca cctgaaagtc
     3841 ggggttctgt tcccgcagac cgactttcgc catttttaaa cctgtcatat tgcccccaac
     3901 atggtggtaa ccatcgccat caatggacca gccagatccg ggtccacacg aaacatcgac
     3961 acaatacctt cactcatctc cttcagtttc tggtggcgtg gtgcgttgag aatgacagcc
     4021 tgttttgcct cactgagttc cttttccatt tcagccaacc tagccatgaa gctatcctgc
     4081 tcaaccaggt aaccgcgata ttccagcggt agtaccgcca gaattgccgg ggtcagttca
     4141 cgcacgttat ttcggtattt ttcagaatcg aatttgttat cgaggaagcg gaacagcttc
     4201 tggcgtgtac ggctgacatc atcagggaaa tcgatggtgc cgccgccctg ctcccgatac
     4261 tcattcacaa tgagtgtggc aacgacatcc tgattatcta cagccgacca ggcgcggacg
     4321 gcatcacgga ttttttcgtg gcctggcacc tgttttattt gagaacgatt tatcaccgca
     4381 gtcgggctaa atccgctagt ctgttggtat gtaagtggtt gcataatcat tgccttatca
     4441 gttaacgccg cagtttaggc ggcagaatta ctcgcgttaa acaatggtgc aaggtcggga
     4501 cgaatatctg ctggtttaat ctttccacca gtggctgaga caattttcat tacatagcgg
     4561 gcatcaattc cgccaccgtg tagccaacgc caaacagtgg gctacaccgc atagatctgc
     4621 cagtcgtttt tgactacctg taatactgat tgcgagttga atggtttgat ttgtcattat
     4681 caattcctat tggtattaca atgaatggat aatagcaatg catattaatc caatcaatag
     4741 caaaacgtgt tttgaccatc aatacgcaag cgtataaatt aaaacttatg aaaaaagaaa
     4801 ctcttgctga tcgcttaaac ctagcgatgg aacaatctgg aatgtctcaa ggcgctcttg
     4861 caaaggcgtc tggcgtagct caacccacaa tctggagact gacaagcggc aacgcacgcg
     4921 gctcaacaaa aattgttgaa atagctaatg cattgggtgt tcgaacagag tggctctcat
     4981 caggcatagg cccgatgaga aatgacggtc aacaatcagg gaagcctgct gtcaaccatt
     5041 ccaaatactt caagattgac gttcttgata tagaagtcag tgctgggccg ggtgtcatca
     5101 accgtgagtt tgtagaagtc ctacgctcgg ttgagtactc gtttgacgat gctcgtcaca
     5161 tgttcgatgg caggaaggca gaaaatatcc gcatcattaa cgtacgcggt gacagcatgt
     5221 caggaacgat cgaaccaggt gatcttctgt tcgttgatat cactgttaaa tctttcgacg
     5281 gtgatggtat ctatgcgttt ctgtacgacg acacagccca tgtaaagcgc ctgcaaatga
     5341 tgaaggataa gctgctggtt atctctgata acaagagcta ctcaccgtgg gacccgatcg
     5401 aaaaagacga aatgaatcgg gtatttatat ttgggaaagt tattgggagc atgccacaga
     5461 catatagaaa acatgggtag attattaatg acagatttca tcaagataac cagatgatgt
     5521 tttggtgaga catgctgaca tcctcataat ttatagaatg gatgtttcaa gcagagctta
     5581 aggtatatgg atatgtcaaa aaaaaatttt gaattcaaaa attacccaat agtctttatt
     5641 ggctcaggca tttcaaaaag atatttagag aactacccaa cgtgggaaga gctattaaac
     5701 gagtattgga aaataacaaa tcccacaaat gatttctata gttatctttt aacaataaaa
     5761 g
//
LOCUS       DAZFDC010000205         5759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFDC010000205 DAZFDC010000000
VERSION     DAZFDC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.205"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(73..606)
                     /locus_tag="ACHJ48_004532"
                     /pseudo
     CDS             complement(73..606)
                     /locus_tag="ACHJ48_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(611..775)
                     /locus_tag="ACHJ48_004533"
     CDS             complement(611..775)
                     /locus_tag="ACHJ48_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709935.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(772..1452)
                     /locus_tag="ACHJ48_004534"
     CDS             complement(772..1452)
                     /locus_tag="ACHJ48_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4709936.1"
                     /translation="MAARLDISREEVLNELWELKKAGFVDKSVYTWRVADNNVQQEQP
                     APEETTTATEAKISECDLTATIEQRGPQTADELATLFGTTSRKVASTLAMAISKGRLI
                     RVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGVALPVQKDATQEDIKTETV
                     ADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKWERVCAALRELNKHRDMVA
                     GICRKSGQ"
     gene            complement(1540..2001)
                     /locus_tag="ACHJ48_004535"
     CDS             complement(1540..2001)
                     /locus_tag="ACHJ48_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4709937.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1994..3034)
                     /locus_tag="ACHJ48_004536"
     CDS             complement(1994..3034)
                     /locus_tag="ACHJ48_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4709938.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(3006..3557)
                     /locus_tag="ACHJ48_004537"
     CDS             complement(3006..3557)
                     /locus_tag="ACHJ48_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4709939.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGAYEYRCTTMG"
     gene            complement(3541..3768)
                     /locus_tag="ACHJ48_004538"
     CDS             complement(3541..3768)
                     /locus_tag="ACHJ48_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4709940.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3845..4252
                     /locus_tag="ACHJ48_004539"
     CDS             3845..4252
                     /locus_tag="ACHJ48_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4709941.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4516..4815
                     /locus_tag="ACHJ48_004540"
     CDS             4516..4815
                     /locus_tag="ACHJ48_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709942.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4888..5106
                     /gene="ydfC"
                     /locus_tag="ACHJ48_004541"
     CDS             4888..5106
                     /gene="ydfC"
                     /locus_tag="ACHJ48_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO4709943.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5129..5536
                     /locus_tag="ACHJ48_004542"
     CDS             5129..5536
                     /locus_tag="ACHJ48_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709944.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5514..5747)
                     /locus_tag="ACHJ48_004543"
     CDS             complement(5514..5747)
                     /locus_tag="ACHJ48_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709945.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         1444 a         1506 c         1241 g         1568 t
ORIGIN      
        1 acctcgtgca tagcttgtta tttaaagtca acaaaataag gaaaattata cgcatattga
       61 agagtataaa ccttacatgt tgattacatt tttgtaatca acatcctgtt tggaatagcc
      121 agcctttaat ggataactat ttctgacaat gcaatgagta taatcaagtc catcttccac
      181 tgagaattag aggcggcatg ctttttccgg ctcttgccgg atatccgtaa ttgtccataa
      241 tctgcagatt tataccttct gcatgacctg tcagcgaaaa tttgtccggt gtttctacgg
      301 gaatgacatc aaaagttaca cgcactcgcg ttaccgtgta gacctacttt cctgcacttg
      361 caagatcaca gtggtgtaac cgtaacagga atttattctc tggaccggca gtaaatccct
      421 gagtggcgtg gttcccatat caatttcccg ccaggcagcc tccattgcca gcgtacaggc
      481 tggagccatg acctgccctt taaatctggc ccgaccatcc caccggacgt gttcttctcc
      541 cctgaactta ggtacagtca tctccagtgg cacaaaagtg tcagcgccat aatttttgac
      601 cgttatcgcg ctacggatat tttgttgact ggtgaaaatc accccgcaga atcaggctta
      661 ttcccttaac ctgggctttc atcctgaccg ccgcctcact acgaccaatc agactgccga
      721 tgcattttac cttcattgtt agtatcatga tttcaggcct gcaccatccg ctcattgccc
      781 ggacttccga caaatcccgg caaccatatc ccggtgcttg ttcagctccc gcagcgcggc
      841 gcagactcgc tcccacttct ggacatgatt cttcgcccta cgcagttcgc gatttgccat
      901 atgcagcgat ggcaaaatca aatcatcttc tcgcgttgca gtaaacgatg gcagcgactg
      961 cacaatgtcc gccacagttt ctgttttaat atcttcctgt gttgcatctt tctgtaccgg
     1021 taacgcaaca ccggctggct gaggaaaggc tttaccatcg gtttccgcta ccgattcaac
     1081 tttcggctct gctggtaaat tatcacctgg tatgcagtaa cgaattttac cgttctggtt
     1141 tacgcgaatc agacgacctt tgctgattgc cattgccagc gttgaagcaa ctttgcggga
     1201 tgttgtaccg aacagcgtag ccagttcatc cgccgtttgt ggtccgcgtt gttcaatcgt
     1261 cgcggttaaa tcgcactctg agattttcgc ttctgttgcc gtggtggttt cttctggcgc
     1321 tggctgttcc tgctgaacgt tgttatcagc cacacgccag gtgtatacgc ttttatcaac
     1381 gaagccagcc tttttcagtt cccacagctc gttcagtact tcttcacgac tgatatcaag
     1441 tcgcgcagcc agctataccg acgtggcttt tcccatcgct ttcagtgcgt caaaaacagt
     1501 ctccataaat ttcctcccgg taaaaaatca cttctcaact caaacaaaac cagccgcttt
     1561 ccggcgttca tattcctgtt tcagcaactc aattggcgtt ggccccgacg ggcgtttggg
     1621 ggccgccagt tgtcgccgga ctggcggaac gctcaggccg ttactaacat gctttgccca
     1681 tttcgtcagt tgccgttctg caagccgttt taattcccct tcggtcatct ggcgctcaat
     1741 cccctttgaa cgcatctcga ggcaaatgtg atacagcaca ggctgagacc acgggtactt
     1801 atcacttccg tcgtatcgcc aggactcatt gcgccagcgg cggtactcct ccatcacagc
     1861 atccaccgtc agaccgaatg gattggctcc gcttttcgaa atcagtgcca caaactcagc
     1921 caggtccgga ggccatgttt cacccgcccg gcagcggtcc atgcactggc ggcagacctg
     1981 ccggatttgc tgctcagtca tcgcgccaat ctgtgcaatc cagagcttcg aaggtgcggc
     2041 cccgttcttc tggatccagc ggttcgaata aaccaccccc atgagttccc acagcttcca
     2101 ggccgtttcc gtcgctgata aatccgtttt cacgttccca ctgctcacgt gctgcccgaa
     2161 tttcctgaac tgcccgtgat gcggtgccac ctggtgctgc tgcatggttt acccctttgc
     2221 tgactggttt aacctgcgcc ctgacgtgat ttacgtgacg ggcgaatttc tgctcccact
     2281 gaatctgcgt aaacactttc ccctccgctg cccagtagtc ccggaaggcg gcaagttcag
     2341 caggtgtaaa ttctgtctcc ggcaaagcca tcccccacaa cgcagcccgt cgtcgaaaat
     2401 cccgtgacgg ataccagcta tcgctcatcg ggaattttcc gatgggttcg ctcaggccat
     2461 acaggaatac agggggggct gcctgtaacg acaaaacttc ctgctcactg gtcggagcac
     2521 tctcgcgtgc gttatgtgtg gggtttagat ctttgggttc ctttgggttc cgtgatccgt
     2581 ttttgggtgt ctttgatgga aaatttgggt gtctttggtt attttccatg cagctaagag
     2641 ttctgttttt gggtctgttt tgtgctgaaa cataaccatt ttcggtactg tttttattaa
     2701 cagcaccaat tttacccacc tttaaagact cccgtttttg ggtgtattca ggctcggcaa
     2761 cactttcttc tacaccgata agtcggtaca ccacaatttg ctttgttctg ccttttctct
     2821 caccggtatc aacaattaac ccaatctcca tcaggtgtcg taagctgtcc tgcacagtct
     2881 ttttgttcag ttccgttact tctgccagtg cagatacaga cgggtatgca cacaaatcgg
     2941 caccgcacat atcagcaagc caggtcaata ctgacttact ggatgaactg ccggttttca
     3001 cctttttagc ccatcgtagt gcatcgatac tcatacgccc ccctggcaga catttgttta
     3061 tctgcaaagt aatattggta ttgctgacga tacgcgtgct tgaaagcaat agctttttct
     3121 ataagctcgt cagtctcacg ttccacaaca actggatccg caaaaagcag cccggactcc
     3181 accacatcgc catattcttt gtttaacccg gcgatcatgt acgtaatgct ttttccgtca
     3241 gtaatttcac aatacaatct gaaatcgctg atccggatag cctccataat tgccggaatc
     3301 agcgccgtga atttgtcacg cttatctctg gtgtcgatag ctttccagcg ttcgaatatc
     3361 ttcacccggt taacgcccag cgcccgttga tcaacctcgc catcattaaa cgtgacgcgt
     3421 tgaacatcga tgttcgggcg ttctttcaga gcccagaatg cttccgtgat taatatcgtc
     3481 gcctgctcct gtgtcattcc tggtcggcat acccaggtat ccagagcctc acaaacctgt
     3541 tcagtggtga ttttcattgt tcaaccgccc cgcccgcttt gccttacgat attcgtcata
     3601 aactttgggg tcgtactgaa gttccccgcc ggatgcctct tgcaggcgca tcgcgcgacc
     3661 ttcaggaacc agttcccccc atgcagcaac gcttgccagc ctaactcctg cgacattggc
     3721 aagctttgtt ttgctgccaa aaaacgctat agcatcaatt ttcaacatat cgaaaccctt
     3781 agattttcct aaggaaacta gatcgtagag aaacctaagt caagaaaaat tagaattacc
     3841 taatatgaaa aacgaaacct tcggtgctcg cctcttatac aggcgaaaaa aattaaaact
     3901 gtctcaggcc gcattaggta agctggtcaa agtggctcac gtaacaattt ctcaatggga
     3961 aagagatgaa acacagccag cggggaagag attattcgca ctgagccagg cgcttcagtg
     4021 ctcgccgact tggcttcttt ttggagatga agataagcaa ccaggcgaac cgatcccaga
     4081 taatcagcca gtcaatctga cagaagatca aaaagagttg cttcaactgt tcgacgcact
     4141 gcctgagtca gagcaaaagg ctctgttgtc agagatgcgt gctcgagttg agaatttcaa
     4201 caaacttttt gaagaactac tcaaagctcg caaaagaagc gcaaataaat aacccccctt
     4261 tttttcgcaa ctctctgtaa taaaaagcac aaactttcaa atacttgtgt tttttacatc
     4321 aagaagctta ggtttttcta cacaaaaagc ttgaccataa ctcttaggct attctaaatt
     4381 ctactcatca agacaccgca cggtgttctc agcaaacagt tccgctaccc ggcgttaagg
     4441 ggaagcagag gatttctcag tgggcgagtc aaacatcaga atggaaggcg tccagggatc
     4501 agcaaagaaa cagcgatggc gctttatatt gatatcagcg ccattgccgg acaggtaaga
     4561 gttatcagag cggtaactaa gcggtatgcg cctttacttc agaaagtctc tggtgagtgc
     4621 accgaagata ttgtcaacga tttcgtcatc gaactgcgag gactcatctt cagttacaag
     4681 gtgaccacaa tttttgcaga tggctcccgc gaaactgtca gagccctgcg gcttaaagga
     4741 tgtgtcaaag acttagccac cacattctgg gcaagaaaac ttgattgtat tcataaccaa
     4801 tttcctctcg agtaacagac ccctcagagg ataccacctc gcctgacgtg gttaaaaagc
     4861 aggcaacgct aaccacaagg agccgacatg cagaaacgag accccgtcat catcgcgcca
     4921 gactataccg atgatgaact ttatgagtgg atgcaccaga aaattaaggc ggtgcaggac
     4981 ctgaaatggg ccaatgaagc cagggctaag caggctgaaa atctgtccgc tctggagcag
     5041 gatatcacca atctggaaaa agcagcggca ttaagcattg ccagaatgat tacataccca
     5101 cgttaatagc taaccaacga ggctaataat ggaatttaaa gatttaccaa cgccattaca
     5161 ggaaatggca tcgaatatag ttcgttcaca actggctact cttgacctta gtaccgcaga
     5221 aaaagaaacc atcgataata tggttcgtaa tgtgcgcaat gctttttctg ggctatatgg
     5281 ttctgataat caaaagcagg aaagcgatgt taataaacgg gtaatttctg tttgcgtgaa
     5341 tggccatgtt ctttcatcaa tcaaaacaga aacggcgaca gtcttcgatt gcctttgcat
     5401 tgtacagagc cttgttgatg ccctgtttcg ttcagtgaat ttagaaaatg atgcaaatct
     5461 gcgagggcgc ataatagcac atccatatgc acatacttta ggctctgtgg atatcaaaga
     5521 tcccacaaat ctttaatgaa atagttaacg cgaattgtac ttgctctttc ggttgctttc
     5581 agaatacgcg ttgaaactgc tggcggtaat ttggtattcc atttattaaa atcatgcccg
     5641 ggaaagtact cttcgaaaat acttttaact gcagactcgc ctattgaaat gctgcttacc
     5701 atgcgatttt gataaaggca tttagcaata agagttgatt ttaacattca ccctcctga
//
LOCUS       DAZFDC010000206         5704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFDC010000206 DAZFDC010000000
VERSION     DAZFDC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.206"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2667
                     /locus_tag="ACHJ48_004544"
     CDS             <1..2667
                     /locus_tag="ACHJ48_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO4709946.1"
                     /translation="GTLNDFLGAMTEDDVRPEALRRFELMVEEVARNASAVAQNTAAA
                     KKSASDASTSAREAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEASK
                     SAAAAESSKSAAATSAGAAKTSETNAAVSQQSAATSASTATTKASEAASSARDASASK
                     EAAKSSETSAASSASSAASSATAAGNSAKAAKTSETNAKSSETAAEQSASAAAGSKTA
                     AALSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQASAAASSASAAK
                     TSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATRQASAAKSSATTASTK
                     ATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSAT
                     NSTSESLAATPKAVKAAYELANGKYTAQDATTAQKGIVQLSNATNSTSEMLAATPKSV
                     KAAYDLANGKYTAQDATTAQKGIVQLSSATNSASETLAATPKAVKAANDNANGRVPSA
                     RKVNGKALSSDITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGA
                     GFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAI
                     GNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIP
                     WPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQ
                     DGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTG
                     ASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVA
                     IGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            2667..3248
                     /locus_tag="ACHJ48_004545"
     CDS             2667..3248
                     /locus_tag="ACHJ48_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO4709947.1"
                     /translation="MAFRMSEQPRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(3368..4258)
                     /locus_tag="ACHJ48_004546"
     CDS             complement(3368..4258)
                     /locus_tag="ACHJ48_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFO4709948.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPLEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVSAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(4277..4783)
                     /locus_tag="ACHJ48_004547"
     CDS             complement(4277..4783)
                     /locus_tag="ACHJ48_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4709949.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            complement(4820..5320)
                     /locus_tag="ACHJ48_004548"
     CDS             complement(4820..5320)
                     /locus_tag="ACHJ48_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO4709950.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            complement(5399..5581)
                     /locus_tag="ACHJ48_004549"
     CDS             complement(5399..5581)
                     /locus_tag="ACHJ48_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO4709951.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
BASE COUNT         1533 a         1364 c         1370 g         1437 t
ORIGIN      
        1 ggtacgctga atgattttct tggtgccatg actgaggatg atgtccgtcc ggaggcactg
       61 cgccgttttg agctgatggt ggaagaggtg gcgcgtaacg cgtccgcggt ggcacagaac
      121 acggcagccg cgaagaagtc agccagcgat gccagcacat cagcccgtga ggcggcaacc
      181 catgcgactg atgctgcgga ctcagcacgc gcagccagca cgtcagccgg acaggccgcg
      241 tcgtcggctc agtcagcgtc ttccagcgca ggaacggcat caacaaaggc tactgaagca
      301 tcaaaaagtg ctgccgctgc agagtcctca aaaagcgcgg cggctaccag tgccggtgcg
      361 gcgaaaacgt cagaaacgaa tgcggcagtg tcacaacaat cagccgccac ttctgcatcc
      421 accgcgacca cgaaagcgtc agaagctgcc tcctcagcca gggatgcgtc ggcttcaaaa
      481 gaggcggcaa aatcatcaga aacgagcgca gcctcgagcg ccagtagtgc agcctcctcg
      541 gcaacggcgg caggcaattc cgcgaaggcg gccaaaacgt ctgagacaaa cgctaagtcc
      601 tctgaaacgg cagcagaaca gagtgcctcc gcagcagcag gctcaaaaac agcggctgca
      661 ttatctgcca gtgccgcgtc aacaagtgcc gggcaggcct cagccagtgc caccgccgcc
      721 ggaaaatcgg cagaaagtgc cgcatcgtct gcttcaacag ccacaacgaa ggctggcgaa
      781 gccactgaac aggccagcgc agcagcgagt tctgcttccg cagcgaagac atccgaaacg
      841 aacgcgaaag cgtcggaaac cagcgcagaa tcctcaaaaa cggctgccgc atcgtcagcc
      901 agttcggcgg cgtcatcggc atcatctgcg tctgcttcaa aagatgaggc gaccagacaa
      961 gcgtcagcag cgaagagcag cgccacgacg gcatccacga aggcgacaga ggcagctggt
     1021 agtgcgacgg cagcagctca gagcaaaagt acggcggaat ctgcagcaac gcgcgctgag
     1081 acagcggcaa aacgggcaga ggatattgca tccgccgtgg cgcttgagga tgcgagcacg
     1141 acgaaaaagg ggatagtaca gctcagcagt gcgactaaca gcacttccga gtcactggcg
     1201 gcaacgccaa aagccgttaa ggccgcgtat gagctggcta acgggaaata caccgcacag
     1261 gatgcaacga cagcacagaa agggatagtt cagcttagca acgcgaccaa cagcacatct
     1321 gaaatgctgg cggcaacgcc aaagtcggta aaggcagcct atgaccttgc taacgggaaa
     1381 tatactgctc aggacgctac gacagcacaa aaaggaattg tccagctcag tagtgcaacc
     1441 aacagcgcat ctgaaacgct tgccgcgaca ccgaaagcag tgaaagcagc taatgataat
     1501 gcgaatggtc gggtaccttc tgcccgtaag gtgaatggta aggcgctttc atcggatata
     1561 acactgacgc cgaaagatat tggtacgctt aactcaacaa caatgtcatt cagcggtggt
     1621 gctggttggt tcaaattagc aacggtaacc atgccacagg cgagttctgt tgtttcaatt
     1681 acgttgattg gtggcgcggg atttaacgtg gggtcacctc aacaggcagg tatatctgaa
     1741 cttgttttgc gtgcaggtaa tggtaatccg aaggggatta ctggtgcttt atggcagcgc
     1801 acatcgacag ggtttacaaa ttttgcctgg gtcaatacat ctggtgatac ttacgatatt
     1861 tacgttgcaa tcggaaatta tgcgactggt gtaaatattc aatgggatta taccagtaat
     1921 gccagcgtga cgattcatac gtcaccagca tattctgcta ataagccgga agggttaacg
     1981 gacggtacag tttattcact ctatacgcca tcagagcagt tttatccgcc tggcgcacca
     2041 atcccgtggc catcagatac cgttccgtct ggctatgccc tgatgcaggg gcagactttt
     2101 gacaaatctg catacccgaa acttgcagcc gcttatccgt caggcgtgat ccctgatatg
     2161 cgtggctgga cgattaaggg caaacctgcc agtggtcggg ccgtattgtc tcaggaacag
     2221 gacggcatta aatcgcacac ccacagcgcc agcgcatcca gtacggattt ggggacgaaa
     2281 accacatcgt cgtttgatta cggcactaaa tccacgaata acaccggggc gcacacgcac
     2341 agtgtgagcg gtacagccgc aagtgccgga aaccatactc atagtgtcac aggcgcatca
     2401 gcagtcagcc agtggtcaca aaatgggtca gtacataagg tagtgtctgc ggccagtgtg
     2461 aatacaagtg ctgcaggagc gcacactcat agtgtcagcg gcacagctgc atctgcaggt
     2521 gctcacgcac atactgtcgg tattggtgct catacgcact ctgttgcgat tggctcacat
     2581 ggacacacca tcaccgttaa cgctgcgggt aacgcggaaa acactgtcaa aaacatcgca
     2641 tttaactaca ttgtgaggct tgcataatgg cattcagaat gagtgaacaa ccacggacca
     2701 taaaaattta taatctgctg accggaacta atgaatttat tggtgaaggt gacgcatata
     2761 ttccgcctca tacaggtctg ccagcaaaca gtacctatat tgcaccgcca gatattccgg
     2821 ctggcttcgt ggctgttttc aacagtgatg agtcatcgtg gcatctcgtt gaagatcatc
     2881 ggggtaaaac ggtttatgac gtggcttccg gcgacgcgtt atttatttct gaactcggct
     2941 cattaccgga aaatgttacc tggttatcgc cggaagggga gtttcagaag tggaacggca
     3001 cagcctgggt gaaagatgca gaagcagaaa aactgttccg gatccgggag gcggaagaaa
     3061 caaaaaacag cctgatgcag gtagccagtg agcatattgc gccacttcag gatgctgtag
     3121 atctggaaat cgcaacggag gaagaaacct cattgctgga agcctggaaa aagtatcggg
     3181 tgttgctgaa ccgtgttgat acatcaactg cacctgatat tgagtggcct gtaatacctg
     3241 aagtttaaaa taaaagcccg cttaaaatat cgcgggcttc gaaatataaa tgtattttca
     3301 gaggctagag cttaggatat ctcagcggca attaacttat gagggatagc taaataccag
     3361 aagataatta gtttttacta tttatctttg tttgtggttc tccttcagca agctcagcgc
     3421 cagtgacagg attgatgtct tcatgggatt tcaaccttgt tgccatagct tttatcatag
     3481 cttcttgctt ggtcgaaaga tttatcgagg ccttcccatc cccaccgtca accgctgaaa
     3541 ctggatcgga aacatagtca aagttttcat cactgttcca actacctctg acgtcttcac
     3601 cttctgacat gttgtaatat acgtttttgt atttctcaag tggtggtaat tttcctggtg
     3661 gaaaggtgtt acgaattgaa tgtaatgctt tttcaaatga aagcatgtgt gctgcttccc
     3721 gggtcattaa aaatgccaga gtgtctttta ctccaggatc atcagtaaca ttaatgagac
     3781 gttcgtaaat gatctttgcc cgagcttcag ctgcaatatt tgaacgaaga tcggccgtga
     3841 cttcgccaat agtatcaaca taagctgcag tccagggtac tccagctgaa ttagttaatg
     3901 ctgggcctcc tccgtagagg agagaagtta tatggctatc attgccattt tgagttaagg
     3961 aacggtaaag ctcagcttca ttctcagttc cttcagcaag tgctcctttt gcacctttgt
     4021 tgagcatacc aacaagagaa ccaataattt cgagatgact tagttcttct gttgctatgt
     4081 ccatcagcat atcccttcgg cctgcatctt catcacttaa gccttgagtg aagtatcggc
     4141 atgctgctgc aagctcaccc tgtggcccac cgaattgttc taagagtaga ttagccaagc
     4201 ctgggtttgg ctcacttaca cgtactgtat attgaagttt tttcacgtgt ctaaacataa
     4261 tgcctcctca aaagaattat tttttggctt caactccatc aatttcagat ctgaccatga
     4321 attgctcagt ggtgtcagga atatggttaa gaagccattc agccatttgt ttttcttcat
     4381 taagaattgc ttcaataatg ggtactgaag ctgtatcacc cgcatttttg gctgctgcga
     4441 gaagagatgt gtaacacgca atttcaaatt gttcgaacac atagccactg atcgaacctt
     4501 tgacaatttc atcagatgga aaaattccac caatggattg tccaaacgcg gccattttac
     4561 tcatagagtc tttaatgaca gagcgcgaaa tattattacg atcaagtatg ctttcaagct
     4621 gtgaaagctg gttctttgtt tcgctaatgt gctgctcaat tctggaacgt aactcaggat
     4681 agttttctat ccggctggcc atagactcaa gcattttttc agcttgtttt tccatcgcat
     4741 gtgcatcacg aagccagtca tggtaatgtt caattctatt cataatctgt ttatcctaaa
     4801 agaaattgaa gtctatagtt caggagttat cctgagcttt tttattaacg ttattaaatg
     4861 ccaggtctgt taatttgaca tcagtagctt tttcctcttc cagtgtttct ttcaggagtt
     4921 tagccgcttt tttatatcct aattgctcag cgagtgttac caaagtgcca taactggcaa
     4981 tttcatagtg ctctactttt tgtgctgcag caatcaatgc agcatcacgt acttcatttt
     5041 tatcagtgct ttcaattacc tcattcgcct cttcgataag tccttccatt gcggcacact
     5101 taattctttt caatttaaga ccatcttcag aatcaacaac ctgatcgatg cgttcaattt
     5161 gaccatgtgt ctcatcaaga tgtgactgaa aagcagcggt taatttatca ctatatgcag
     5221 agcgactaag tttagacagt gcctttgtta gttgtttctc agcgctgtat gtatccgata
     5281 ataagtggat gaagatatct tcaacagatt taatttgcat aatcactcca gagttttcac
     5341 tgcgagcccg taggctcgca gttagcttca gttattcacg aatgtagggg gtgataaatt
     5401 aagagtcaga tttacggccg ccaccatggc tattctgacc accttttttc cctgcttctg
     5461 atgctctttg tggatcgttt ttaaaattac cgccactatg ttggccgcct ttacgaccag
     5521 cgtcagaagc tttttgtcga tcttcagcga aattacctgc accgccacga tgttcggtca
     5581 tatcatttct cctctgattt tcaaattgta aataacatgt gccttgtcag cacccaatta
     5641 actctagtaa caaataagaa agtgtcaaat aagagattgg tatgcgcatc ttaatgtgag
     5701 aaat
//
LOCUS       DAZFDC010000207         5579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFDC010000207 DAZFDC010000000
VERSION     DAZFDC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.207"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            224..1156
                     /gene="rfaD"
                     /locus_tag="ACHJ48_004550"
     CDS             224..1156
                     /gene="rfaD"
                     /locus_tag="ACHJ48_004550"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO4709952.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1166..2212
                     /gene="rfaF"
                     /locus_tag="ACHJ48_004551"
     CDS             1166..2212
                     /gene="rfaF"
                     /locus_tag="ACHJ48_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO4709953.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLKELNALLLQEEA"
     gene            2216..3208
                     /gene="rfaC"
                     /locus_tag="ACHJ48_004552"
     CDS             2216..3208
                     /gene="rfaC"
                     /locus_tag="ACHJ48_004552"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO4709954.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGENQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3205..4413
                     /gene="rfaL"
                     /locus_tag="ACHJ48_004553"
     CDS             3205..4413
                     /gene="rfaL"
                     /locus_tag="ACHJ48_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO4709955.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4449..>5579)
                     /locus_tag="ACHJ48_004554"
     CDS             complement(4449..>5579)
                     /locus_tag="ACHJ48_004554"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO4709956.1"
                     /translation="IIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGY
                     SNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVH
                     NSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIE
                     IVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAH
                     KNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLV
                     VIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDI
                     LKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         1533 a         1230 c         1264 g         1552 t
ORIGIN      
        1 tgcttccgtt atttatcata catcggaata ttgatactaa agcactattc acatgcaaaa
       61 ccaacatccg ccatgaagga ctagctaaaa cccaaactag tttgttgcaa ttagcatcct
      121 tgcacctcta tgtaaagggc tgaagggatt cggatgtgat ggtatgatta cagacattcg
      181 tgtctgagat tgtctctgac tccataattc gaaggttaca gttatgatca tcgttaccgg
      241 cggcgcgggc tttatcggca gcaacatcgt taaagccctg aatgataaag gcatcaccga
      301 tattctggtg gtggacaacc tgaaagacgg caccaagttt gtgaacctgg tggatctgaa
      361 tatcgcagac tatatggata aggaagactt cctgatccag attatggctg gcgaagagtt
      421 cggcgatgtc gaagcgattt tccacgaagg cgcgtgctct tccaccaccg agtgggacgg
      481 caagtatatg atggataaca actatcaata ctccaaggag ctgctgcact actgcctgga
      541 gcgtgaaatc ccgttcctgt acgcttcttc cgcagccacc tacggcggac gcacctccga
      601 ctttattgaa tcccgcgagt acgaaaaacc gttgaacgtc tacggttact caaaattcct
      661 gtttgatgaa tatgttcgcc aaatcctgcc ggaagcgaac tcgcagattg ttggcttccg
      721 ctatttcaac gtttatggac cgcgtgaagg ccataaaggc agcatggcga gcgtcgcttt
      781 ccatctcaac acccagctta ataacggtga atcaccgaag ctgttcgaag gtagcgagaa
      841 ctttaaacgc gacttcgtct atgttggcga tgtggcagat gtaaacctgt ggttcctgga
      901 aaatggcgtt tccggcatct tcaacctcgg caccggtcgt gcggaatcct tccaggctgt
      961 agctgatgct acgctggctt atcacaagaa aggccagatc gaatacattc cgttcccgga
     1021 taagctgaaa ggccgctacc aggcgttcac tcaggcagat ctgacaaatc tgcgcgcggc
     1081 gggttacgac aaaccgttca aaaccgttgc tgaaggtgta acggaataca tggcctggct
     1141 gaatcgcgac gcataagagc tctgcatgaa aatactggtg atcggcccgt cttgggttgg
     1201 cgacatgatg atgtcgcaaa gtctctatcg cacgctccag gcgcgctatc cccaggcgat
     1261 aatcgatgtg atggcaccgg catggtgccg tccattatta tcgcggatgc cggaagttaa
     1321 cgaagctatt cctatgcctc tcggtcacgg agcgctggaa atcggcgaac gccgcaaact
     1381 gggtcatagt ctgcgtgaaa agcgctacga ccgcgcctac gtcttaccta actccttcaa
     1441 atccgcatta gtgcctttct tcgcgggtat tcctcatcgc accggctggc gcggcgagat
     1501 gcgctacggt ttactcaacg atgtacgcgt gctcgataaa gaagcctggc cgctaatggt
     1561 ggaacgctat gtcgcgctgg cctatgacaa aggcattatg cgcacagcac aagatctgcc
     1621 gcagccattg ttatggccgc agttgcaggt gagcgaaggt gaaaaatcat atacctgtaa
     1681 tcaattttcg ctttcatcag aacgtccgat gattggtttt tgcccgggtg cggagtttgg
     1741 tccggcaaaa cgctggccac actaccacta tgcggagctg gcaaagcagc tgattgatga
     1801 aggttatcag gtggttctgt ttggctctgc gaaagatcat gaagcaggca atgagattct
     1861 tgccgctttg aataccgagc agcaggcatg gtgtcggaac ctggcggggg aaacacagct
     1921 tgatcaagcg gttatcctga ttgcagcctg taaagccatt gtcactaacg attctggcct
     1981 gatgcacgtt gcggcggcgc tcaatcgtcc gctggttgcc ctgtatggtc cgagtagccc
     2041 ggacttcaca ccgccgctat cccataaagc gcgcgtgatc cgcctgatta ccggctatca
     2101 caaagtgcgt aaaggtgacg ctgcggaggg ttatcaccag agcttgatcg acattactcc
     2161 ccagcgcgta ctgaaagaac tcaacgcgct attgttacaa gaggaagcct gacggatgcg
     2221 ggttttgatc gttaaaacat cgtcgatggg cgatgttctc catacgttgc ccgcactcac
     2281 tgatgcccag caggcaatcc cagaaattaa gtttgactgg gtggtggaag aagggttcgc
     2341 acagattcct tcctggcacg ctgccgttga gcgagttatt cctgtggcaa tacgtcgctg
     2401 gcgtaaagcc tggttctcgg cccctataaa agctgaacgc aaagcgtttc gtgaagcgct
     2461 acaagcagag aactatgacg cagttatcga cgctcagggg ctggtaaaaa gcgcggcgct
     2521 ggttacgcgt ctggcacatg gcgtaaagca tggcatggac tggcaaaccg ctcgcgaacc
     2581 tttagccagc ctgttttaca atcgtaagca tcatattgca aaacagcagc acgccgtaga
     2641 acgcacccgc gaactgtttg ccaaaagttt gggctatagc aaaccgcaaa cccagggcga
     2701 ttatgctatc gcacagcatt ttctgacgaa cctgcctaca gatgctggcg aatatgccgt
     2761 atttcttcat gcgacgaccc gtgatgataa acactggccg gaagaacact ggcgagaatt
     2821 gattggttta ctggctgatt caggaatacg gattaaactt ccgtggggcg cgccgcatga
     2881 ggaagaacgg gcgaaacgac tggcggaagg atttgcttat gttgaagtat tgccgaagat
     2941 gagtctggaa ggcgttgccc gcgtgctggc cggggctaaa tttgtagtgt cggtggatac
     3001 ggggttaagc catttaacgg cggcactgga tagacccaat atcacggttt atggaccaac
     3061 cgatccggga ttaattggtg ggtatgggga gaatcaagta gagtgtcgtt ctacaagtat
     3121 gtctcttgca gatttgccag ctcaaaccgt ttttcaaaat ttaaatcttg aaataataac
     3181 caataagttg acatcggaga taagatgacc tcaacattat ttttctctct cgagaaaaaa
     3241 aactggatag cgtactggaa cagagctctc gtattcttat tcattaccac ctattttttg
     3301 ggtgggatta caaggtataa acatcttatt gttattctta tgacaataac gacaatcgtc
     3361 tatctctgca aacggccaaa acactatctc tcactattta aaacatttct ttttggtagt
     3421 gttgccatat taactattgc tgcattgctg tcacttcttc aatcccctga tgcaggtgct
     3481 agcatgaagg aaattttcaa agctattatt gagaatactt tactatgcac aatagcaata
     3541 ccgatcatat tgagagacga gaaaagagaa gatgtcgaaa aaatcgtttt cttctcattt
     3601 attagtgcgt tgggcttacg ctgtttttct gaattgatta cctattataa ggactatcaa
     3661 caagggataa tgccattcgc agattataga caccgtagca tttctgactc gatggtcttt
     3721 ttattccctg cattgttaaa tctctggctt atcaaatcag caaaataccg catttctttt
     3781 gtggttctaa gcgttatttt tatttttctg atattaggaa ctttatccag aggggcctgg
     3841 ctttccgtgt tagtcattgg attaatatgg attctgatgt ttaaacaatg gaagttacta
     3901 ttagtaggag taatggttgc catcattgca ttgtcggtta ttttcacaca taaggagatg
     3961 accgcaaagc taacgtataa acttcaacaa actaatagtt cttatcgcta tgcaaatggt
     4021 actcaaggca gcgcactcga tctaatatta gaaaatcctg ttattggtta tggttacggt
     4081 aacgttgcat ataaagatgt ctataataaa cgtgtcattg attatccaga atggaccttt
     4141 agacaatcaa tagggccaca taattttgcg ctattcatct ggtttggcac tggtttatta
     4201 gggctggtaa gtcttatgat gctatactgt gcaatattga aagagtgtat aaaaaatggc
     4261 gtcaagaata aatatcgctc accatataat gcatattata taatcttact atcttttata
     4321 ggttattttg ttatccgtgg aaacgtagaa caaattgaac caaatttatt aggcgtttac
     4381 gccggcttat tattagcgat gaaaaacaag taaaaaataa aaaaggctgc ataatgcggc
     4441 ctttttattt attcaaacca attatgaata acctcttcga atctttgagt tacgccatcc
     4501 caactgtagt gatcaaacac aaagtcctga ccttgctttg caactgctgt taattcagga
     4561 ttcgctaacg tttttaaaat atcgctgctt atggagtctg cagtcatagg ttcttttaaa
     4621 tggaatcccg tagtattttc tttgacaaat tccgtcatcc ccccacgcgt actcactaaa
     4681 acaggcttac cagcacccat agcttcaatt gccaccatgc aaaatggttc ctggaactgg
     4741 gaaggaatga cgactaaatc tgccagtggg taataacaat gcatcttttc aggcggtatg
     4801 cttcctaaca taatgcaacg atccttcaaa cgttttgcaa tttccctgac attgcgttga
     4861 tatgcacctt tgtcactttt actcatttct gtataatcac caatcacaac taattttaaa
     4921 tttttgtgag cagcggccaa tttttcgaat gattgcatca acagcaatat gcctttgtca
     4981 gggacgatac gcccagcata aaaaataatt ttctcttcag gggagatatt aagttcgctt
     5041 ctagtgatcg gctgaaaatt agattgatag gtttctaaat caattccatt tggtacaatc
     5101 tcaatatctg catttgctaa ataagattgg taatactttt tcaagtataa actaggcact
     5161 atcattttaa cattttgctc aagcaatttt ggttcaaatg cattatgcat atgtataacc
     5221 actcttgcct gaggagctcg cttacgaatt tgagtgtata gcttcatgct attatgaact
     5281 acaataacac tttctttcgt tataggaaaa tcatgtgcaa tattcaatat tctctgtgaa
     5341 tatggaagag gatcaagtct tgtccatttt tgaaataacc gcttatatat gcgactgaat
     5401 ccaaccctat gaatactaca attatcatta acaaaggtgt aatttgagta tcctggattt
     5461 ttgatacaaa caatacgatt tggaaaattg gtacgctgag caacctgata catccacgtt
     5521 tcaacagcag ccgctccgcg aggaggaatt gaaaaaatag gagtaaccgt aaatattat
//
LOCUS       DAZFDC010000208         5360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFDC010000208 DAZFDC010000000
VERSION     DAZFDC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.209"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..81
                     /locus_tag="ACHJ48_004555"
     tRNA            6..81
                     /locus_tag="ACHJ48_004555"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHJ48_004556"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHJ48_004556"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHJ48_004557"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHJ48_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO4709957.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHJ48_004558"
     CDS             complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHJ48_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4709958.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHJ48_004559"
     CDS             complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHJ48_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO4709959.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3595..4620)
                     /locus_tag="ACHJ48_004560"
     CDS             complement(3595..4620)
                     /locus_tag="ACHJ48_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO4709960.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5051..5272)
                     /locus_tag="ACHJ48_004561"
     CDS             complement(5051..5272)
                     /locus_tag="ACHJ48_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709961.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1471 a         1383 c         1288 g         1218 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttatgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgact taggatgcgc aaaaaactca tccggtgcag
      541 cttgctccac tatttcgcca cgatccataa aaatcacccg gtcagcgacg gttcgcgcaa
      601 accccatctc atgggttaca cacaacattg tcatacccga ttgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctccggat cgagcgccga tgtgggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgagcgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctca aggtaatgca
      841 tcgccagagc ttcagcctct ttctttggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtag aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatgctggct atagcgcgac atgctgaaac agaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcttgc
     1381 tgaacgctac tgaaaagatc gaacaacccg ataatgatca ccaggctggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaacccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggcagcg cctgtaatcc acctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcagtttg tcgatactgg tgccttctgc cataaacaaa
     1681 ggcagcatga ccgaagacat aaacagaacg gtaatcagcg gcacgccgcg ccagaattcg
     1741 ataaaaatga ccgataagat gcgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaactgatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagagcaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcactgcc cagaccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgattgac acaaggccaa tcagtaatgc caagttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagtcca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc cgggcgcgag ggatgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttccacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctggttca gcacggtgcc agcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggtgg aataatcacc
     2581 cgcagtgcct ggggaataat gacctggcgt agcgtaaccg ggttgggtaa tcccagtgac
     2641 cgagccgctt catgttgacc ataaggcact gcctggatcc ccgcgcggat aatctcggcg
     2701 ataaatgcag aggtataaac cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa
     2761 accatcccgc cgcggaagtt aaagccttgt agggctggga catcccagtg tagtgctgcg
     2821 ccaaaaagcc attgcgccag taaaggcaaa ccgatgatca acactgcggc gatgggccag
     2881 gtgcggcgca gctgtccggt ctttatctgg tatgttttat taaagcggaa tagcccaaca
     2941 gaaaggacta tagccataac aacagccagg ataaacgcaa taaatccgtc tcccagctgc
     3001 ggtgacggaa tataaagccc acgattgctc aaaaaggcca gatcaaacgc gctcacagcc
     3061 tggcgtggtc cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc
     3121 gggggaatat tacggaagat ctcaatataa attgtggaaa gctttcgtag cagccagtta
     3181 tccgaaagtc tcgccagacc gataaagaag cccaaaacag aagcgaacac aatacacaat
     3241 gcagaaacca gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc
     3301 tgctggtaat cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca
     3361 aaacctgaag taatgccacg attattgaga ttcgttacag tgttgtgaaa taaccaacca
     3421 acaatgccga caacagcaac aacggcaagg atctggaata accaggcgcg aaccgtaggg
     3481 ttggcaaaag agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa
     3541 agcataacgc tgaagagagc gccgccgtag cgacgctcgt tacctcacac cgacttaacg
     3601 cacgggcggt gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat
     3661 tttcagcggg ctttctgaac ctacgttacg ctcgaaaatt tccgagtagt tacccacctg
     3721 tttgatgatg ttataggccc atttattatc caacttcaga tccttgccgt aatcgccttc
     3781 tttacccagc agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt
     3841 ctgggaattg atgcccatct cttcagcatt tagcatggcg aaaagcgtcc agcgtacaat
     3901 cgagaaccat tcatcatcgc cacgacgaac taccggccca agcggttctt tagagataac
     3961 ctccggtaag acaatccatt cagccgggtt gcttaatttg atgcgcaggg catacagttg
     4021 ggattgatcc gaagccagcg tatcgcagcg accggattcc agtgccttcg ctgattcgtc
     4081 agaacgatcg aaagtcactg gtgtgtactt catattgttt gccttgaaat agtcggcgac
     4141 gttgagttcg gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc
     4201 gctttttagc cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc
     4261 agtaaatgcc attcccatcc cggcatcgcg agatgaagtc caggtcgtat tacgggagag
     4321 caaatccact tcccctgact gtaaagccgt gaagcgttct tttgctgtga gtggggtata
     4381 tttcactttc gtgtcgtcac caaatacagc agcggcaaca ccacgacaaa catcaacatc
     4441 aatacctgaa aacttaccgt ctgcatcggc ataagagaac ccaggtaatc catcactgat
     4501 cccgcattgc acaaaacctt ttttctgaac ggcatcaagc gtcgcgccag catgcgcctg
     4561 atttgcaacg gcaagcagca cgctggcggc agccagtgtg gctatcatca tctttttcat
     4621 aatgcttcct gtgaggcgaa aattatcgtt ataggacgtt tgggaaacgt cttttgcctg
     4681 tctgtggcca tcctcttata agcaaaagta gtgccagctt ttgcgcacga tttcgcgtcg
     4741 caataagctg agtgtagaca ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg
     4801 aaacatgcgc catattttgg cgcaaaacat ttacgcgtgt tttactaagc agaataatga
     4861 ttgttagcgt gagtatattt ctggtgggaa gtgcgaaagt taaattgatg cgttatttcg
     4921 aaagcagcga aataacacga tataacatgc taaatcagca gagaattagc cagcagaata
     4981 tagcaatatt ttatcttatg taaagatact gtatcgttaa acagagcgcg aaactccgcg
     5041 ccctgttcag ttatttcgac agagcgatga tccccatcgt caggccagca acagccgctg
     5101 ccccgccagc aatagcccca gccgtttccc agttatcctg agtcgtgcct ttcatacagg
     5161 tattggtgtg aaccagacga ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag
     5221 cacagcccgc cagcaatgcg gtgagcaatg ccacaggaat taatcttttc atggtgttac
     5281 ctcagtaata aacgctaacg cttaataagt taactctctt attaacgagc tgaatgccat
     5341 tttatcaccg gaggttataa
//
LOCUS       DAZFDC010000209         5307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFDC010000209 DAZFDC010000000
VERSION     DAZFDC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.210"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..206)
                     /locus_tag="ACHJ48_004562"
     CDS             complement(<1..206)
                     /locus_tag="ACHJ48_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO4709962.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDA"
     gene            complement(190..432)
                     /locus_tag="ACHJ48_004563"
     CDS             complement(190..432)
                     /locus_tag="ACHJ48_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4709963.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEDVPWKWALLIQA
                     VTAGALKYELHIPTVVIPDSDHNPPSNQGGIHENQA"
     gene            824..1162
                     /locus_tag="ACHJ48_004564"
     CDS             824..1162
                     /locus_tag="ACHJ48_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO4709964.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRIVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            1455..1607
                     /locus_tag="ACHJ48_004565"
     CDS             1455..1607
                     /locus_tag="ACHJ48_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4709965.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            1619..2257
                     /locus_tag="ACHJ48_004566"
     CDS             1619..2257
                     /locus_tag="ACHJ48_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709966.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(2258..2467)
                     /locus_tag="ACHJ48_004567"
     CDS             complement(2258..2467)
                     /locus_tag="ACHJ48_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709967.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            3031..3219
                     /gene="dicB"
                     /locus_tag="ACHJ48_004568"
     CDS             3031..3219
                     /gene="dicB"
                     /locus_tag="ACHJ48_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4709968.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            3216..3404
                     /locus_tag="ACHJ48_004569"
     CDS             3216..3404
                     /locus_tag="ACHJ48_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4709969.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            3497..4735
                     /locus_tag="ACHJ48_004570"
                     /pseudo
     CDS             3497..4735
                     /locus_tag="ACHJ48_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
     gene            4723..5274
                     /locus_tag="ACHJ48_004571"
                     /pseudo
     CDS             4723..5274
                     /locus_tag="ACHJ48_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1553 a         1196 c         1212 g         1346 t
ORIGIN      
        1 gcatcagggg tgtctttctc tatccagcgg aaaattttct gggtattgcg agccagggct
       61 tccggatggc tgtcgtcgta cagttcagga aacgtcatac ccaactcaaa ataagcctgg
      121 gttattccag ctgccggaac tttttcgcca tcaggacgcg cccaggcatt catcgccatg
      181 cggatgtgtt catgcttgat tttcatgaat ccccccttgg ttagaaggcg gattatgatc
      241 agaatcggga atgacaaccg tcggtatgtg taactcatat ttgagcgccc cggcagtgac
      301 tgcctgaatt agcaacgccc atttccacgg aacgtcttcc ccccacatgc tgactgtggt
      361 ttttgacgtt cctagagcgg cggctgtttt aacaactccg ccaaaatagc ctaatacttc
      421 tgattttttc atgagtcgct ccataaaact gaacgccaaa agtttaataa tcaaaaccaa
      481 agaaagtcaa gaaacaaaac catctgtgtt ttaaaatcaa aacatgaaca agcaaacaat
      541 atctgaacgc ataacccaac gtatgcatgc gctaaacttg aaaggcaaag accttgtcaa
      601 tgccactggc gcatcaaaag gctccgtaag tcaatggatg aacggtggag gagcgccgtc
      661 ctcgcgttac ataagttcac tggcaaagat attgaaagta aacgaaaatt ggcttcttaa
      721 tggaggagag ttaaatacag gtgattcgct tgatctatct ttaccgccga taaaaacggt
      781 tccgctacta tcacttcagc aggcagcaag ctggagtgat tatatgaaaa attcctcaat
      841 aacctcttgt gtgcagcttg tcggagaaat cccggccaat acctttgctg ttgttctaga
      901 gagtgacagt atgtcgacat ctggtggtgg agtttccatc ccaaatggtt caacagtttt
      961 tgttgatccc gatcgaatcg tacaaccagg aaatattgtc cttgccttac ccaaagggac
     1021 cacaacgcct gtcattcgca aactggagat agaagggccg gatattcttt tagtccccac
     1081 gaatcctcgc tacccttcaa ttatgctgga tgatctatct tgcatattgg gagtatgctt
     1141 taaaattcaa caagatattt aaccaacctc atctatttga ttaactgtat gccatcgtag
     1201 tgatggcgta acagctgcct gcttaaaatg ttttgataaa aaaacattga ccttaaatgt
     1261 tcgtttttct aaacttcatt cattccctca ccccgcccca cagaatgcag ggcaatactt
     1321 cgagttacca ggcagtggtc aggggttaag tagccagccc gaggcgtaag aacatgacgg
     1381 cagggttcaa ctttaataac tatgcagcag gtttttgttc cgctaccccg gcgttaaggg
     1441 gaaatgaggt caacatggat acgctcaatc ttggcaacaa cgaatctctg gtatgtggcg
     1501 tgttccccaa ccaggacggt acgttcaccg cgatgacgta taccaaaagc aaaacgttta
     1561 aaactgaagc tggcgcgcgt cgctggttag ccagaaacac tgactgatga ggttgacgat
     1621 ggaatttaaa gatttacctc cttcaatcca ggagattgca gcacacacac ttcgtcatcg
     1681 tctgaacgaa cttgaattgg aatcggtaac gaaaaaagac actgataata tggctcgtaa
     1741 tgtgcgcgat gcgtttaccg gattgtattt ctgtgcgtct ataaataaac acgactcaga
     1801 gagtgtggca aataaaattg cagaaacgac agcgcaaaac atcaatacga aaccaacgga
     1861 agaagaaatt gatcagtttg ctcatgatgc tggtttaaaa aacaagaaag aaaaatcgcc
     1921 atatgcgggg aacatgtttg tttatgacaa tctcatcaga attcgtggcg aaattccggc
     1981 ggaatacctg gcaagagtcc atcaggcatt gcttaaaaat ttggaaacag aattatttga
     2041 tggcaacact aacggtttct tcatggtatc aggccttgag aaagactggg atgcagaaaa
     2101 acgctggaat gttgctacat ggttattcag taacagagcc gctgcactgg aagcttcggc
     2161 atgtatttgc ggcctgttct taacagacca caaatataat ctggatgtgt acagttatat
     2221 ttacgctgaa cacggtccac tctggattga ctggtaatta taaggaaaca ccagcagggc
     2281 cgcggcgacc aacagaacga ttaaaatcaa taatgccatt ataaaggaca ttatttaatt
     2341 tatcgtcgaa tgctgattct gtgagcctca actctgaatg agtttttaat aaccctgatt
     2401 gcctgagttg atttaccagg cattcaatct gtttttcaat aagcggattt ctttttttgt
     2461 ttggcatttt atcctccatt gaggttctgg gttaaaaatg gagaccaaca cgctgtcacg
     2521 tgtggtcgtg cgccggacac ggataagaat ccggtactga cagtttactg aaaggatatt
     2581 tccctgaaaa gtcagggcat aacgcgaaag cgtacggcga agctctttcc cttagaaggc
     2641 ttgtcgttag atttcttcga acgtgcgctt ccggttgtgg cactccgcga aatggcgcgg
     2701 cggtaagtat ggcggggtta ttccttcccc ttgaggacac cgggttgtca ggttgaccat
     2761 acgcttaagt gacaaccccg ctgcaacgcc ctctgttatc aattttctgg tgacgtttgg
     2821 cggtatcagt tttactccgt ggctgctctg ccgccctttt taaagtgaat tttgtgatgc
     2881 ggtgaatgcg gctaagcgca cgcggaacag ttaaaaccaa aaacagtgtt atgggtggat
     2941 tctctgtatc cggcgttaat tgttaactgg ttaacgtcac ctggaggcac caggcaccgc
     3001 atcacaaaat tcattgttga ggacgcgata atggaaacgt tattaccaaa cgttaatacg
     3061 tctgaaggtt gttttgatat tggtgttctg ctcagtaacc gggagtttac tgaagatgcc
     3121 attaatatga ggaaatatga gccttatctg ctcaatgata attccatact ttcccgaatt
     3181 gctcttcttg aacttggtat tttcggagaa cgtcaatgac ttcagcattt gcactgatga
     3241 tgacggtttt tcttataacg ggtgaatcac agaatgtgat taccggaatt tatgccagta
     3301 aagaatcctg cctccaggca agagacgagc aaaaaatttc tggtgaatgc ctcccggtaa
     3361 aaaaagtatc gctgtacctg aataacgaaa caccggctgg ataaccctcc agccatatta
     3421 acaccatacc aacggattaa aaatgccagc aatggcaggg atttgttcac ccttaaatct
     3481 gtaatgaggt ttatcaatga gcactgataa agaagaattt gcgctatatt gcgaagcaaa
     3541 aaatgacaaa gtcagaaaac gtctgggaat taaaggtggt ttttactgga ctacagcaaa
     3601 aaaattatct gttgccatct cccgctgcat taccgcaatg gatgacaacg attatgatga
     3661 agacgacttt aaaaaacccg ttcgcgtcca tttacccgtt gtgaatgacc ttccacctga
     3721 aggcgtgttt gataccgaat tctgcaaccg atacgaaaaa ggcggggaag atggcattac
     3781 aatggtattt atcgcgccct ctccctccgt gcaggagaaa ccagccagta ctgacaatac
     3841 caacgtcaac ggcgaagaca tgacggagat tgaggagaat atgctcctgc cggtttctgg
     3901 tcaggagctg cccattcgct ggcttgcgca acacggcagc gaaaaaccag taacgcacgt
     3961 tgcacgggaa gaacttcagg cattacatat agcacgggcg gaagaactgc cggctgttac
     4021 tgccctggcc atttctcaca aaacaaagct gctcgacccg ctggagattc gcgaccttca
     4081 caaactggta cgcgacactg acaaagtttt ccctaatccc ggtaattcag acctgggact
     4141 gataactgct tttttcgagg catacctgga cgctgactac actgatcggg gtctgctgac
     4201 aaaagagtgg atgaaaggaa atcgtgtttc gcgtatcacc cgtacggctt ccggtgcaaa
     4261 tgccggtggc gggaacaaaa ccgatcgcaa tccgaattta gtacacacct tcgatacgct
     4321 ggatgtggag attgcagcag ccacacttcc gatggatttt aatatttatg aaattccggg
     4381 cagcgtttat cgtcgcgcaa aagaaatcgt cctgaaaaga gaaagtccgt tcaaagaatg
     4441 gtccgcagca cttcgcgcaa ccccgggtat tctggactat tcccgcgccg ctatttttgc
     4501 acttatccgg agcgctcacc ctgagtttta tcactacccg ggacgccttc aggggtatat
     4561 caacgcctac ttaacggaga ctgatcacga gaaccccagc aaggaaactc tcactgctgc
     4621 acgacataca ccggaaaaag atatcctgga agaagttaac cgcgaactgt ctgctaagca
     4681 ggaaacagaa gaagaaaatg atgaagaaaa accgcaacca tcttgcgcaa tggcagaatg
     4741 ataatcagaa gaaatctggc aaaagaccgt tttacagtac caaaaacaca ggcgggaacc
     4801 aatcgtgtga ttcaccttat taagcccgca atcgacgctc tccggagtca gatggcacta
     4861 acgagactga gcaaagagca tatcatcgat gttcacctca gagaattcgg cagaacagag
     4921 aaacaaaaat gtacctttgt ttttcaacct gaagtgtcag cgaaagtaaa aaattatggc
     4981 gaccatttta ccgttgactc aataaggcag atgtgggacg cagcggtaaa gcgtgccgga
     5041 atccgccatc gcaaatcgta tcagtcgaga cacacttatg cctgctggtc gttaacagca
     5101 ggagctaacc cggcattcat tgcaaaccag atgggccatg cagatgcgca aatggtgttt
     5161 caggtgtacg ggaaatggat gtcagaaaat aataatgcgc aggtaacgct gttaaataca
     5221 cagttaagcg agtttgcccc aaccatgccc cataacgaag cgatgaaaag ttaatttttt
     5281 atttatcaat tagttagatt gaatgac
//
LOCUS       DAZFDC010000210         5288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFDC010000210 DAZFDC010000000
VERSION     DAZFDC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.211"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            119..1477
                     /gene="mltD"
                     /locus_tag="ACHJ48_004572"
     CDS             119..1477
                     /gene="mltD"
                     /locus_tag="ACHJ48_004572"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO4709970.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(1525..2295)
                     /locus_tag="ACHJ48_004573"
     CDS             complement(1525..2295)
                     /locus_tag="ACHJ48_004573"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4709971.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQLLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALCDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(2373..3173)
                     /locus_tag="ACHJ48_004574"
     CDS             complement(2373..3173)
                     /locus_tag="ACHJ48_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO4709972.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3414..4328
                     /gene="yafC"
                     /locus_tag="ACHJ48_004575"
     CDS             3414..4328
                     /gene="yafC"
                     /locus_tag="ACHJ48_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO4709973.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPVACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4325..5128)
                     /gene="dkgB"
                     /locus_tag="ACHJ48_004576"
     CDS             complement(4325..5128)
                     /gene="dkgB"
                     /locus_tag="ACHJ48_004576"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO4709974.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGLAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1384 a         1270 c         1319 g         1315 t
ORIGIN      
        1 ctgaagagcg ttttgcatgg ttaaggtcaa agaaagatag gttctgataa aactttcttg
       61 tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa gcaactattg acgcacacat
      121 gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg ggttgccaga gtaccggcaa
      181 cgttcaacag cacgcacaga gcctttctgc agctggtcaa ggggaagcag caaagtttac
      241 aagtcaggca cgatggatgg acgatgggac gtctatcgcg ccagatggtg acttgtgggc
      301 tttcattggc gacgagctaa agatgggaat tccggaaaat gaccggattc gcgaacagaa
      361 acagaaatat ttacgcaata agagctatct ccacgatgta actttacggg cagagccgta
      421 tatgtactgg atagccgggc aagttaaaaa acgtaacatg cctatggaac tggtactact
      481 acccatagtg gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg ccgcaggcat
      541 ctggcagatc attccgagca cggggcgcaa ttatggtttg aaacagaccc gcaattatga
      601 cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg aatatgatgc agcgtctgaa
      661 caaaatgttt gatggcgact ggcttctgac cgtagcggct tataacagcg gcgaaggtcg
      721 ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt
      781 accgttgccg caggaaacga agcagtacgt gcctaaaatg ctggcattga gtgatattct
      841 caaaaacagc aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc
      901 gcgtgtgcac ctgagtagcc cggttgaaat ggcgaaggtt gcagatatgg cggggatttc
      961 cgtcagcaag ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg gcgcaagtgg
     1021 tccgcagtac gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat ctctggcttc
     1081 aggcgaaatt gctgctgtac agtcgacgct ggttgccgac aatacgccgc ttaacagccg
     1141 tgtttacacc gtacgctctg gcgacacgct ttcaagtatc gcttcacgtc tcggcgtaag
     1201 caccaaagat ttgcagcagt ggaacaaact gcgcggctct aagctgaaac caggccaaag
     1261 tctgacgatt ggcgcaggta gtagcgcaca gcggttggca aacaacagcg atagcattac
     1321 gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct aaacgccacg gcgtgaacat
     1381 caaagatgtg atgcgctgga acagcgatac tgcgaatctg caaccaggcg ataagctgac
     1441 gttgtttgtg aaaaacaaca gcatgccaga ctcctgacaa accagataat agaaaggcac
     1501 cgattccccc ggtgcctttt ttatttatgc cgctttatgt gcatctacca tgatgatatc
     1561 actggtgaaa gagccatcat tctgcaaggc aaaatacgtt ctcacctctg tcgatgcgct
     1621 ctgttggtaa atgcgaatag cgtctactaa cgcttctggc gtacgcattc tcgcgaccca
     1681 tgaagaaaat tccagcggta acttatctgt aattaaatta tcaactatca gattggcttc
     1741 attgattaac ctcaaccact caccgctggc gtagtttcgt acgtgagagg tatcgcataa
     1801 tgcttctacc gtctgtaacc agatgtcgcg cactgggtgg cccggagaca ttacgtccat
     1861 cacaatcagc ctaccgccag gtttcaatat ccggttcact tcacgcagtg ctgcaccaac
     1921 atcatgccag tgatgggcag aataacggct gataacaata tcaaatgcgt tatcggcaaa
     1981 tggcagactt tcggcatacc cctggcgggt ggtgatattt ttcagttgcc ggacttcggc
     2041 agcttgtgcc acgacatcca gcatttgggc agataagtca tacgccacca ccgtgctcac
     2101 attttgcgca gcgacaaagc tggcatgccc tgctccgcag cccatatcaa gtacacttgc
     2161 atcaggataa tcagccagac acaccgccag gagctgtaaa tctcgtccgg atgcatgtac
     2221 ggtactggtc agatattcgc tggcttgaga gctaaactgc ttttctacgt ggtcatggtg
     2281 ggattgtgtt gtcattgcac tgtccttttg ttgtttgagt ataaaagggc agctccaaac
     2341 gttggtctgc cctgtggcag acctgacata ccttatttat caggcttgcc gggactgaat
     2401 tcaacgagta gcggattgtg atcggacgcg cgcgtaacca gtacagaagc ttcgctgacg
     2461 ttcagaccac ggtagaaaac aaaatcgagc gggcgaccaa acgcccgacg gcgctgatca
     2521 tcggtaaaac gcacctgacg cagcgacatt tcccgcgcaa aacgatataa cgcgttcatc
     2581 cttctacggc tccaagcatt gaaatctccc gccataatga ccgggccgct gtggtgagct
     2641 atctgatcac caataggaag taactgctta ctataaacat ccacgcccag actgaagttg
     2701 acggcgtgta tattaaccac cattaacagg cgggtgtcag gtaatggata gaccgtcacc
     2761 agtgccgact tcgccagacg caaaatgggt tctcgttcac gtaacgggca gcaatacact
     2821 ggatgtgccg ccgaaagggt cattacgcca gaaggatgtt gtggcagcac gaaagcgggt
     2881 acctgatcgg cggcaagata gttagcagtc gcaaactgta ccaactctgg cgttgtttgc
     2941 gcttcctgca ataacaccag atgtgcgtct ttgccgtaat tctttaatac cgacaaccat
     3001 tcagcgcgtt gctgtttgta tatgttccac accaggatcc gaatacgctc ttcggtactt
     3061 aacggttctc cgggtggtaa tgcctggccg atgctcgcaa aagaccccgg cggtaagatc
     3121 ctctccgcag gttgtccggc aacatagcgc atggcatagg tgttttttcg cacttttgac
     3181 tttgaaacct ctattacgtc aacccttccg cactcgggag ggttctactg ttatagggat
     3241 tttagctcac tctttcaacg agcaattgta tattgttatg taagcaagtg cttacaccgg
     3301 tcagaatttt gctcattatt gagaacatta aatcggatga catatcacgc atcctgatgg
     3361 tttaatgaca aataaattca aaatatctca tctattttgc aacaggagca aaaatgaaag
     3421 ccacgtcgga agaactcgcc atttttgttt cggtcgtcga aagcggcagc tttagccggg
     3481 cagcggaaca attagggcaa gcaaactcag cggtaagccg ggcggtgaaa aagctggaga
     3541 tgaaacttgg cgttagcctg cttaatcgga ccacgcggca acttagcctg acggaagaag
     3601 gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga gatggcagcg gcagaatcag
     3661 aaattatgga gacgcgtaat acaccgcgtg gactgttgcg catcgatgcc gcaactccag
     3721 tggtgctgca ctttctgatg ccgttgatta agccttttcg tgaacgctat ccggaagtca
     3781 ctttatcgct agtctcctcc gaaacgatta ttaatttgat cgaaagaaaa gtggatgtcg
     3841 cgatacgcgc cggtacgtta acggattcca gcttacgtgc caggccgtta tttaacagtt
     3901 atcgaaaaat tatcgcctcc cccgattata tttcccgcta cgggaagcca gaaacgatcg
     3961 acgatttaaa gcaacatgtt tgcctgggat tcactgaacc cgcttccctc aatacctggc
     4021 cggtagcctg tagcgatgga caattacatg aggtgaagta cggtttgtca tccaatagtg
     4081 gggaaacact gaaacagctt tgcctgagtg gcaacgggat tgcgtgtttg tccgactata
     4141 tgatcgacag agaaatcgct cgcggagaat tggtggagtt aatggcagat aaagtgttgc
     4201 cagtggaaat gccattcagt gccgtctatt acagcgaccg tgcggtaagt acgcgcatcc
     4261 gggcttttat cgatttcctt agcgagcatg taaaaacagc tcccggagga gctgtcagag
     4321 aggcttaatc ccattcagga gccagacctt ccgggctaac caggcggtcg ttgcaatcca
     4381 gtgcggcgat cgctttttta tcttcggcat caagctgtaa attttgtgcc ttaagattac
     4441 tctccaggtt ttcacgttta gtagaagaag gaattactga gtaaccttcc cccatagccc
     4501 acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc tgcgatacga gcaataacct
     4561 catctttcag ggccttacca tacgccagcg tcatatagga agtaatatgg atgccgtgct
     4621 gtttagccca ggcaaccact ttacggtttt gcagataagg agagagttca atctggttag
     4681 tagcgatgtt ttcagcgcca acagcagcaa tcgccttttc catcaatggg atcgtgaagt
     4741 tggaaatacc gatctcacgc gtcaaccctt cttttttggc ttccagcagc gcctgcataa
     4801 actcttcaac agagacttca tcgtttggtg acggccagtg gattagcgtc agatcaacat
     4861 aatcggtacg caatttttgc aggctctctt ttagactcgg gatcaatttg tctttgctga
     4921 gattttcaat ccagatttta gtggtgatgt agagttcatg acgtggcacg ccactttctg
     4981 caatcgccag acctactgcg gcttcgttat catagatttg tgcagtatca attgcgcgat
     5041 aaccaagttc aagcgccgtt ttcacagatg aaataacaac gtcgtctttc agacggaaag
     5101 tacctaaacc aaatgcaggg atagccataa tatgcctctt ttaatgctat tttcgttaac
     5161 tgaggtgatt atgggcttta gaatgttgcg gaaaaagggc gtaaaacgca gaggattttt
     5221 gcgattttag caataataga aatgtagacg aaaaaaaacc tcgagcgata actcgaggct
     5281 tcttaata
//
LOCUS       DAZFDC010000211         5250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFDC010000211 DAZFDC010000000
VERSION     DAZFDC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.212"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1412)
                     /locus_tag="ACHJ48_004577"
     CDS             complement(<1..1412)
                     /locus_tag="ACHJ48_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4709975.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSSSGAEATGVTTLLSYLASESEGSLKVQGWSASGGR
                     AEVVSDAEGTGGKAVKLTKEA"
     gene            1656..1979
                     /locus_tag="ACHJ48_004578"
     CDS             1656..1979
                     /locus_tag="ACHJ48_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO4709976.1"
                     /translation="MNWIVIDTVIQPACDISFSAIWCNIKLILWYQSDIFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            complement(2277..2546)
                     /locus_tag="ACHJ48_004579"
     CDS             complement(2277..2546)
                     /locus_tag="ACHJ48_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO4709977.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(2558..3517)
                     /locus_tag="ACHJ48_004580"
     CDS             complement(2558..3517)
                     /locus_tag="ACHJ48_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO4709978.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            complement(3608..3684)
                     /locus_tag="ACHJ48_004581"
     tRNA            complement(3608..3684)
                     /locus_tag="ACHJ48_004581"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3648..3650),aa:Arg,seq:tct)
     gene            complement(3698..3774)
                     /locus_tag="ACHJ48_004582"
     tRNA            complement(3698..3774)
                     /locus_tag="ACHJ48_004582"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3738..3740),aa:Arg,seq:tcg)
     gene            complement(3784..3859)
                     /locus_tag="ACHJ48_004583"
     tRNA            complement(3784..3859)
                     /locus_tag="ACHJ48_004583"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3824..3826),aa:Ile,seq:cat)
     gene            complement(3900..4958)
                     /locus_tag="ACHJ48_004584"
     CDS             complement(3900..4958)
                     /locus_tag="ACHJ48_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO4709979.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(5110..>5250)
                     /locus_tag="ACHJ48_004585"
     CDS             complement(5110..>5250)
                     /locus_tag="ACHJ48_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709980.1"
                     /translation="ATAPATVGEIAQNTYLSRERCQLILTQLVMAGLADYQFGCYRRL
                     QS"
BASE COUNT         1326 a         1464 c         1159 g         1301 t
ORIGIN      
        1 gcttccttgg tcagcttcac tgccttacct ccggttccct ccgcatcgct gaccacttct
       61 gccctgccgc cactggctga ccatccctgt actttcaggc ttccctctga ctcgctggca
      121 aggtaagaga gcagtgttgt gacgcctgtg gcttctgcac cggaagacga tgacgggcgc
      181 acctctgata ctgtcgatga tgcccccgcg tttagcgcca ctcttcccgc atggcgcaaa
      241 atcgccgttg ccagacggtc ggaaataatc ccgcggcgag cccatgaact gaaatggctc
      301 gccctgtcct gtgacgtcca ggtggctgag ctgtcacgcc atttcgaacc gtaatacccg
      361 atacccggaa tgtccgggtc ttcttccggt ttgttcgtcg gcacattcac cccgttctca
      421 tcggtcatga acggtacgaa atggatattc ttttccgttt tgtttttgta gctgccgtac
      481 accgtctggt acgaggattc gttcttctgc ttccagaaat acgtcgtatc tccacagatc
      541 cagggaacac cgccagcaga gccaccaacg cactgacctg ccatatccgt caggtctgca
      601 cggaatttat caaccagcgc accaaactgt gctgcgtgat ttccgggcgt gccgtcaaaa
      661 tcaaattccc cctgcatcca caccacggca aacagcacat ttttcgggtt cttcttcagt
      721 gctgcttttg ttcgaccgat aaggtcctta tacagcggct tgtccacacc ccagcgggtt
      781 gaattctccg aggcaccact cgcgtcactg tatgtgccat cagctccggt ggtgaacgct
      841 gaaccaccac gacagcacgg aaccagcaga atgcccgcat tcgccggtat aaacggcagc
      901 agttttttgg cgatatgcag cccctgcccc acggtaccgt actgcccttt tgacaggtcc
      961 gctttcggat ggttaagacg gctcatgtcc tgcacatcat gcagacaatg gtccgccgga
     1021 atgatgtcgt tatatttgca tgctgcaccg cccggtgtca ccgtactgcg gcgcgccagc
     1081 tgcttaatac gcgggtccgg acggtcatat gtctccggca gcggaagacc ttcaccatac
     1141 gacatgccat ttgactgccc tgccagaacc acaacaaagt aatactccgg gtcgctggtg
     1201 gcactgacag ccaccgcctt accctcatta ccggtcaccg ccactggtgt ggtgacatca
     1261 ccttccgccg caatggcctg catcagggta taaggcgtga tggccaccgg actgccaaat
     1321 ggctgccagc cctccttcag tttttgtgtc agtcgttcag caaggtctga cggcgatgcc
     1381 gccctgacca catcgtagtg tttaaatgtc ataacctctc ctgccgccag caaagtaatt
     1441 atccggaata cacaatcatg taacacataa aaaacacgct ataaaaaaac catgtctctt
     1501 atataacaaa aaaacgccga caatttaaaa tgtcctctta ttttaaacaa agaataaacg
     1561 acaccagaaa aacaactcag gctgacagat tgctttcaaa acatcactta taatttatgg
     1621 catcatttaa aaccttaaga aaataaggaa agcaaatgaa ctggatagtg attgatacgg
     1681 taatccagcc agcatgtgac atatcatttt cagccatatg gtgtaacata aaattaatat
     1741 tatggtatca gtctgatatc ttcctccctc cgggctgtat ttttacacca acccggacag
     1801 gcgtaatact caataataaa gagctccccg tcaccattta caacgtgacc ccatttaata
     1861 aaaacgtctg gaatttaatt aaaagcagcc aggaatgccc tgcaaacaca ggaaatatca
     1921 cagataagtg ttttaatcat cgctgcattc tggaaatatg cccatacggg cgaaaataac
     1981 agaatcattc agataaaaag cccctccgga gaggggctga agccgcattt ctgtatcacc
     2041 atgaacatgg tgccgggtgc ctcccggtga gttcagtccg gtgtccctga acccgcgtat
     2101 ctcgatccag gttgtcgtca gagatgacac cttatacacc agtcgcccct ccgcacaggg
     2161 ggattcacca tgcgaaattt ttttaacaaa tgctcagtct gacaggcaac tgtcaactga
     2221 ctgaattgtg acacagatta cacttgttac ccacatacca cgaatcaggt tatgcctcag
     2281 tcattattaa actgcacttc agcaaatccg gagcctgatt cacaggtact ggatttgatt
     2341 gtgacagtca ttcctgtcaa ctgagcactt tgcagtaacg gttgcagatt ccagcgactg
     2401 gtccagtatt ctttcccgtc aaccttcact gtaaatgtgt catcctcatt atacttggaa
     2461 aactcaattt tacctttagc acaatccgcc gccattgcat taacagaagc taatgcaaat
     2521 aaaaccgcca taaacatctt cttcatactt aactccttta tttacccgtt gtatataaaa
     2581 actgtgactt tctgttcaga aacgctgcag ctgtattact ttcccataat gtattgttta
     2641 tttttataac gggcctgtcg ccagttatct gacattctgg ttgactctct tcattcacgg
     2701 cgcgaacaga acgcgccccc tgatgatggc aattcagtat aacggccaca gtacccagta
     2761 tcgctgatat attattaaag gatattctcc ccactctgac accatcctct ccccgatact
     2821 ccggaagcac attgctgatt cgcccccagt tcagagtgag gtccacgtct cccggcgtca
     2881 tcgtatacac aggagcagtt tcagacagtg cctgacgaaa ttctctctgt atctgcctga
     2941 agcgtaaggc ttctgccgtg acagtgacaa aacgcagaac tgctctggat gcatctctgg
     3001 tcattgtatt accactgaac tccattaacg ccagatatga tgaaaccagt gagtgacgac
     3061 tgatttgcat tccggaacgt tccagcgctg cgacacgttg cagagtggta taactgctgt
     3121 ccgttgtcat ggaaaccgtt gtcacatcgg gcactgatat atgtgtaaaa tctgaaaaac
     3181 ggtagaaagt atttgttgcc gtattaacga acccggccac atataaatta ttttgctcaa
     3241 taatcagacg aagatggtca aaacgcgcct gatagacatc aagccctcgt atatccacag
     3301 caaaataact gcccggcggg gtgtggttaa taacagacac cgatgtggtc ccctgagata
     3361 tatgttcaag aggggtcgat atctctgtcc gtatactatt taacgaagag acataacttt
     3421 gttgagtcga aaagtctatc gtaaattccc gggaatagga taccgaagaa aaacccagta
     3481 acaggcacag tacccattta aataatatac acttcatata caggtgttcc ttttggctga
     3541 agtaatcagc accagacccg gcgcagatat aaaaaaggcc cgcaaaagcg agcctggtaa
     3601 ataaatatgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga aggcaattgc
     3661 tctgtccggc tgagctaaca acgctgaata ccgataatgg accgccatcg aggactcgaa
     3721 ccccgcgcag ccagcttcga aggctggcgc tctgtcccga tgagctaatg gcggtatgtg
     3781 atatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc gctcgctctc
     3841 accactgagc taaagggccg ggcgcaggat aataacggta cgtaactaat cctgcaatat
     3901 catccgttct gactggctaa atcctgaact tccctgaccg tctgctcaaa acgttcagtc
     3961 tccagctcaa cgccagttgc acgacgcccc agcgccagtg cagctttgac tgtcgaaccc
     4021 gaccccatga aaaaatctgc aaccaggtca cccggacgac tgctcgcgct gattatctgc
     4081 tgcagcattt ctgccggttt ttcgcacgga tgtttcccgg gatagtactg caccggttta
     4141 tgcgtccaca catccgtgta cggcacctgc gccgtcacgc caaaataccg ccgcagatgc
     4201 ttatattcac tctgcagttc cgcatactgc cggttcagtg aagtatacgt ctccagcagc
     4261 tggtggtggg gcttttccag ttcaccgcgc tgatgcttct cttctgccac ccgggcaaac
     4321 agcgactgta atttcagata atcgctttcg ttcggtagct gccactgact ggcactgaac
     4381 cagtgcgaca ccatgttttt ctttcctgtg gcatccacta tctgttttgc cgttatcccc
     4441 agggcagcac gcgcatcacg aaagtaagca atcagcggag ccatcacatg ctgtttcagt
     4501 gccctgccct tcgcctcata cccggcatct ttcggacgat acggcccctg ataatgttcc
     4561 gcgaacagaa tgcgctctgt ggcggggaaa tacgccctca ggctttcctt gttgcacccg
     4621 ttccagcgtc cggacggctt cgcccagata atatggttca gcacactgaa gcgttcacgc
     4681 atcatgattt caatgtcaga tgccaggcga tggccacaga acaggtaaag acttccggca
     4741 ggtttcagca cccgccagaa ctgcgccaga cactggtcca gccatttcag gtaatcctca
     4801 tcacccgtcc actggttatc ccagccctca ggcttcactt taaagtacgg cgggtccgtg
     4861 actatcaggt caacagaatt ttcgggtaac gaccggataa attccaggca gtcggcgttg
     4921 attaactcac aactggatat ttttacagta ttaaacatgg atcattaagc ctgtctctga
     4981 taggctcata ccgcttttgc gcaaagcaga tgggcctgag gtttgcttgt gaccccaacg
     5041 catgagcaga tggctggtga gtgcccctaa cacccaccag ccgcccattt accacaaata
     5101 aaaaagcctt caggactgaa ggcgtctgta acaaccaaac tgatagtctg ccagacccgc
     5161 cataacaagc tgggtcagta ttagctggca gcgttcgcgt gaaaggtacg tattctgtgc
     5221 aatctccccg actgtcgccg gtgcggtggc
//
LOCUS       DAZFDC010000212         5228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFDC010000212 DAZFDC010000000
VERSION     DAZFDC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.213"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..379)
                     /locus_tag="ACHJ48_004586"
     CDS             complement(<1..379)
                     /locus_tag="ACHJ48_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709981.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLD"
     gene            complement(436..831)
                     /locus_tag="ACHJ48_004587"
     CDS             complement(436..831)
                     /locus_tag="ACHJ48_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4709982.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(847..1616)
                     /locus_tag="ACHJ48_004588"
                     /pseudo
     CDS             complement(847..1616)
                     /locus_tag="ACHJ48_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1642..2382)
                     /locus_tag="ACHJ48_004589"
     CDS             complement(1642..2382)
                     /locus_tag="ACHJ48_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4709983.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            complement(2389..3351)
                     /locus_tag="ACHJ48_004590"
     CDS             complement(2389..3351)
                     /locus_tag="ACHJ48_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4709984.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQNFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(3374..3799)
                     /locus_tag="ACHJ48_004591"
     CDS             complement(3374..3799)
                     /locus_tag="ACHJ48_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4709985.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(3796..4098)
                     /locus_tag="ACHJ48_004592"
     CDS             complement(3796..4098)
                     /locus_tag="ACHJ48_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4709986.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            4196..4567
                     /locus_tag="ACHJ48_004593"
     CDS             4196..4567
                     /locus_tag="ACHJ48_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4709987.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            4588..4779
                     /locus_tag="ACHJ48_004594"
     CDS             4588..4779
                     /locus_tag="ACHJ48_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO4709988.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            4781..5059
                     /locus_tag="ACHJ48_004595"
     CDS             4781..5059
                     /locus_tag="ACHJ48_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO4709989.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT         1257 a         1454 c         1153 g         1364 t
ORIGIN      
        1 catccagagt taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg
       61 acgaaccctt tttttcccga aagtttccag cctgaagcat ggcggcgctg tctggcgggg
      121 cagcatatag cggcacgtat atttccggtt ccttatcagc accgggttgc tcttccagtg
      181 agaatgtctt tccggtaaat cgattcatat aaagcacggg ctctgcttcc agcgaagcct
      241 gagcaacaag ggccagtgct aaatccaact caattgcctc gagagaattt ttgaatgctg
      301 tctgttttac tgcaaatttc atcgccttta cattttcact aacatgactg attaactgct
      361 cttttgtaaa agtggtcatc tcattctcct ttgatgcgaa tgccagcgac aattgaagcc
      421 tgatagctaa ttcactcaca gtaccgcctc ctgaaaattg ccctgataga acgccagtac
      481 acgcagcata acttcactct tccggcactc gccacagatt atgttctgtt gtctgtcgta
      541 gcggcgtatt tctccgtctg gtaactttcg aatcaatgtc tggtcggttg ctttctccgc
      601 tgccttacgc catacgcgat acacctgttc tgatgtaaaa acaccgtatt taccgggcat
      661 gtataaatcg ccacaagcca gtacatccac aaggcaacgt ctgactgaat gccagcctgc
      721 tcccgtcgct ctctccagtt gtgatatcgt catgcgttca tttttgcgta ccagcccgat
      781 aattcgggcc ttcagttctt cacgctgttc gtgtgtaaaa ggtttcgcca taagcgcctc
      841 cggctatcac ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc
      901 ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc tcccacttct gaacatcact
      961 tttcgccctg cgcagcgcca ggtttgccct gcgcagggac ggaaaaatca gctcatctgc
     1021 ttgcgtttcg gtaaacgatg gcaacggctg cacaatgtcc gccacagttt ctgttttaat
     1081 ttcttcctgt gttgcggctt cccggactgg taacgcagca cctgctggct gaggaaaggc
     1141 cttaccatca tttttcgtta ccggcgcggc tttcggatct gctggtaaat taccccccga
     1201 catgcagtaa cgaaatttac cgttctgatt aacgcgtgcc agccgccccg ttgcggttac
     1261 caccgccagc gtggaagcaa ccttgcgagt actgacgccg aacttacccg ccagttcctc
     1321 acacgtttta gcaccatcct gaccgataaa ctcaaccatc atgtctgcgg taacttttgg
     1381 agcgacctct tcggttacca catccggcgc ttcaggttgt agtgcctgcc cttcggttac
     1441 cccggcttca ccttcgacag ccagaaacca ggtgtgaccc gttttatcaa caacgccatt
     1501 tttttgagtt cccacagttc gttaagaact tcttcacggc tgatatcaag ccgcgccgcc
     1561 agttcaacag aattggcttt tcccatcgct ttcagtgcat gcaatacagt ttccatcgaa
     1621 aatttacctc gtcaaaaatt ctcacatacc ctgacgtcca acgtttgacc gccagctctc
     1681 ccagttaaaa ttcacccaac gaccaccatt catggtcata cggtccatca cccgatctcc
     1741 gaggagtgtg ctcatcgctg cgtgattcag gttcgtcagc attccgacac tacgcatcga
     1801 agccgttctg cggtcgacta tctggttcag cgtgacctgc tcgttgcgcg tatcccgctg
     1861 catgccaatt tcatcaagga ccagaaggtc aactccacaa agctcctgta aaaatttttc
     1921 cccggactgg ccgttgtcgt agccgtcatg caacacgctc atgacatcgg acacggtgac
     1981 gataatcacg cttctcccct tcgccatcag ccgattgcca atcgctgctg ccaggtgatt
     2041 tttaccggta ccaggtttac cgctgaacac gaagtttgta catccggtca tcaattcatc
     2101 ggcaatggat ttcgcctggc tcagagcatg gcgctgaccg tcgttctgca cccggtagtt
     2161 cccgaatgag cacttcctgt gaagcggctg gatgcccgca cggttcagga ttttttcaac
     2221 ccgcacctga tgattcaggc ggttaatctc ctcgctgcgt tttcgtcctt cagcaagttg
     2281 ccattcccgc cactcctcca ccgtccggta cggtggaacc gccccctgtg gtgcaagtct
     2341 gcgaatacgt tcaagaaccc caactgccgc aatgtttttc atgacacgtc accccctgaa
     2401 tcccggcggt atttcagtgt ccggttcaga aatgtgattc acgcaacgct gcgcgggcga
     2461 acgccccagg cggataacca gttcatccca tttttcccgg agttttgccg gactcatgat
     2521 gttttttacc cagaacgaat cccgctggag acgcccaaac atttcacaaa tttgtctgtg
     2581 agttctgcca tccagcatcc gcattgtccg cacgtcattg gcccatgcag tccagttggg
     2641 ttctttcggt ctcgtgatct cgccatcatc gctggccgcc tgctcgtaaa gactcacgat
     2701 tcgtccccag atccactgtg cgcacaccaa atcttcctga cttccccact ggcgtttttt
     2761 cgcactgaac acaaccgcgt cagggtgtcg ggttaaaaaa ttctgttcag ccgtctgcgg
     2821 gtccggttgc gaagcgtccg gacaagaaga tcttttatct gacggatcag gttttaatac
     2881 tgacggatcg gggtcaatca tcgcccccct aatcggcagt ttgttatcaa cagttgatcc
     2941 atcaaaattt gacgggtcaa ccgttgaggg ggcaatattt gacgggtcaa ctgttaacgg
     3001 gtcatttttt gccgggctaa tttttctttt cggtttatat gactcacgcg ccgccgcagc
     3061 agctgcttcg agtttttcca cattaagccg atagatattg cttacattac gcccaccgac
     3121 cttacgctct tccttcgtca gccagccctc tttcgccagt tctgcaatag ccgatttcac
     3181 tgtggattca cttcttgcac cgatctgacg ccggatagtt tcaatggcag gccatgacac
     3241 gccctcgtca ttgctgtagt ctgcaagacg ggccataacc gccaccctgg ataagatcat
     3301 gccggtgaag gcgcaccctt cccagacaag accatgaagc ttgctgctca taaaaccccc
     3361 gaacaccgtg cttttagtgc atcaccacag cattccctgc cgggccgccg cgattcatct
     3421 ggtcatacaa aacaaccgct gacgcaacaa aatcatcgac atccttcacc agccgatccc
     3481 tccgttcgac gatctcacgg taatattcag aactgtggct gcgcatacgg gccaccagca
     3541 aaggcggcat cgccttttcg atcgccggta acagagcctg cattttttca acagcatcag
     3601 gggtgtcttt atccagccaa cggaaaattt tctgggtatt acgggccagg gcttccggat
     3661 ggctgtcgtc atacagttcc gggaacgtca ttcccagctc gaaatacgct ttggtaattt
     3721 tcgcagccgg tactttttcg ccgtccggat gcgcccagac attcatcgcc atgcggatgt
     3781 gttcatgctt gattttcatg aatcaactcc atcagataag catgcactac aatcaccttc
     3841 agcatgaact acatgtgttt gccccaaacg aatgccgctc gcatactcag gccaaataag
     3901 ctcccaatca tggggtcgta gctccgccct acttacttgg ccttccgtcg cagattcgat
     3961 cataagggcg cgggttggag atatagctgt tcgtccagac gccatttgcg ataagtaaga
     4021 tggcgataca ccaagtctgg ccgcgaattt cttagcatca ccaaccctta atgatttaat
     4081 aaactctttt aatgtcatac cttcctcggt ttagtgtttt tttgcgagtt tagtgtttaa
     4141 taaaccatta agtcaagtat ttgcttgttt agtgattact aaagataatt accacatgca
     4201 aaaaaaagaa attcgccgtt tacgtctcaa ggagtggttt aaagataaaa ctctgccacc
     4261 caaagagaag agctacctat ctcaactaat gagtgggaga gcctcgtttg gagaaaaggc
     4321 tgccagaaga atagagcaaa catacgggat gccggaaggg tatctggatg cggaatacgc
     4381 agaacaaccg gggggttctc caccacatgc agggttaacg tctaatcaac tggaattatt
     4441 gcagattttt tcagccttcc ctgaggatga gcaacgccag ataatcagcg agttaaagca
     4501 gaaaaaagaa tcaatggaag atctcatagc gagatggatt gcggcgcaaa aatgccgccg
     4561 cgcctgagtt ataaaaccgg aggaaacatg aatagagccc tttcaccaat ggtttctgaa
     4621 tttgaaacca ttgaacaaga aaacagttac aacgaatggc tgcgtgcgaa agtagcaacg
     4681 agccttgcag atccgcgccc agcaattccc catgacgaag ttgagcgcag aatggcagaa
     4741 cgctttgcta agatgcgcaa ggaacggagc aagcagtaaa atgttacccg tgttatggct
     4801 tgaaagcgca gataccgacc tagatgatat aactagttat attgctcgtt tcgacataga
     4861 tgcggctgaa cgcttatggc agcgattaag gggttgtgtg ctgccgttat ccgaacatcc
     4921 gtatttatac ccaccaagcg acagagtacc tggcttgcgt gagattgtag cccaccctaa
     4981 ctatataatt ctataccgcg taacaacatc aagcgttgaa gtagtaaacg tgatccacgc
     5041 aagacgccag tttccctaac tttcactacc aatagaaaca taacaaccgc aacgacttta
     5101 tcaaaagcgt tgtgtttgtt atgtcccgcg gtttagtttt tacttgactt aagtttaatg
     5161 tttattaaac taaaaatacc aacccacccc gccccacaga acgcagggaa atacttcgag
     5221 ttacccgg
//
LOCUS       DAZFDC010000213         5063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFDC010000213 DAZFDC010000000
VERSION     DAZFDC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.214"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            168..671
                     /gene="ftnB"
                     /locus_tag="ACHJ48_004596"
     CDS             168..671
                     /gene="ftnB"
                     /locus_tag="ACHJ48_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO4709990.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(751..1002)
                     /gene="yecJ"
                     /locus_tag="ACHJ48_004597"
     CDS             complement(751..1002)
                     /gene="yecJ"
                     /locus_tag="ACHJ48_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO4709991.1"
                     /translation="MSQTLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1117..1203)
                     /gene="azuC"
                     /locus_tag="ACHJ48_004598"
     CDS             complement(1117..1203)
                     /gene="azuC"
                     /locus_tag="ACHJ48_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO4709992.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1465..1788
                     /gene="yecR"
                     /locus_tag="ACHJ48_004599"
     CDS             1465..1788
                     /gene="yecR"
                     /locus_tag="ACHJ48_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO4709993.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGISDRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1959..2456
                     /gene="ftnA"
                     /locus_tag="ACHJ48_004600"
     CDS             1959..2456
                     /gene="ftnA"
                     /locus_tag="ACHJ48_004600"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO4709994.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2493..2732)
                     /gene="yecH"
                     /locus_tag="ACHJ48_004601"
     CDS             complement(2493..2732)
                     /gene="yecH"
                     /locus_tag="ACHJ48_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO4709995.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2924..4135
                     /gene="tyrP"
                     /locus_tag="ACHJ48_004602"
     CDS             2924..4135
                     /gene="tyrP"
                     /locus_tag="ACHJ48_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO4709996.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4197..4862)
                     /gene="yecA"
                     /locus_tag="ACHJ48_004603"
     CDS             complement(4197..4862)
                     /gene="yecA"
                     /locus_tag="ACHJ48_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO4709997.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT         1236 a         1229 c         1162 g         1436 t
ORIGIN      
        1 agcattttct tatacccgtt cagacgttat tcttatttca gatcatcgtc agaattgact
       61 ccacgatcac atttcggacc ggcagaaagg aattattctg caaacagtaa ttatggtgtt
      121 ttgatttatc ttgcacctct ccacttctgg atataaggat attaggtatg gcaaccgctg
      181 gaatgcttct caaactcaac tctcaaatga accgcgagtt ttacgcatcc aatctctacc
      241 ttcacctgag taactggtgt tctgaacaga gtctgaacgg caccgccact ttccttcgcg
      301 cccaggcaca gagtaatgtg acccaaatga tgcgcatgtt taactttatg aagagtgtcg
      361 gcgctacccc catcgttaaa gccattgatg ttcccggtga aaaactgaac tctctggaag
      421 aactgttcca aaaaacgatg gaagaatacg agcaacgttc tagtacgttg gcacagttag
      481 ccgatgaagc gaaagaactg aatgatgatt caaccgtcaa tttcctgcgc gatctggaaa
      541 aagaacagca gcatgatggc ctgttgctgc aaaccattct tgatgaagtg cgcagtgcga
      601 aacttgcggg tatgtgccca gtgcagaccg accaacatgt tctgaatgtc gtgtcgcatc
      661 agctgcattg atcatacatc gacgctaatg cattgcaccg atgaagtttt tgagaaaccg
      721 ctgcctcatc ttttttgaag cagcggtttt ttaatgggat tcgccttgtg gggtaaactt
      781 gagctcaatc agcgcgatgg ctttttggat tgcccgcatg gtgaccgggt ctgcggcagc
      841 gggatgatga gtaaagtcga tattcttcag ctgactggac attttttcac gaacttcaac
      901 gggcgcgatt acatcgagaa catccagaat ttgtttgata accaactggc aagcaaccac
      961 atcagaaacc agttcctgat cggcattcag cgtctgggac atcgtaaact cctgatagca
     1021 ttttgaaagc cgttatagta gcggcttcac atcttcaacg atagtcacat ccaccctcat
     1081 caggacacaa aaaaacctgc cggagcaggt tttttgttat cggaacatat tgcctggcgg
     1141 tacgtctttg aacgtcttgc aatagttatt gaacatactt ttcaggattt tgcgcagttt
     1201 catcgcggca ctccgaccat ttgttataca ggtgttattg tctttgcgct tataatatga
     1261 caaccatcac aaaaatcaat ctttatgtga tacaaatcac ataaataccc ctttaatgtt
     1321 ataaaaatga taatcaaaga acagccccct atttctgaca cctacagatg gcaagaaata
     1381 gcgcctgcca ggcgtctttt ccggccattg tcgcagcgct gtaacgcgta aaatagtgct
     1441 ttctcttact cttctggctg gaccatgaga cttctgattc tgactctttc attaataacg
     1501 ctggcggggt gtacggttac tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt
     1561 attgtacggt tggtttatga ccaggctttt ttgcagcatg cccataccga tcgttatgtc
     1621 agtcgcggga tctccgatcg cgcatgccaa caggaaggct atacccacgc gatccccttt
     1681 ggtcagccag taggcaactg tagccttttt gcgggttcct tatgtttgaa tacggaattc
     1741 actttgtcat atcagtgcca tcattccgcc ttccctgtct ttctctaaat attttaatca
     1801 gcgaggggat cttcgctgat taaagaaata gcggtaatgc gttttattcc cattcgtatt
     1861 tttaataatt gaagtttata ttttaccttt tgcaaataat aaaataacaa attatagtga
     1921 cgccacagca acaaatataa cctttgtgga gcactatcat gctgaaacca gaaatgattg
     1981 aaaaacttaa tgagcagatg aacctggaac tgtactcttc actgctttat cagcaaatga
     2041 gcgcctggtg cagctatcat accttcgaag gtgctgccgc gttcctgcgc cgtcacgccc
     2101 aggaagagat gacgcatatg cagcgtctgt ttgattacct gactgatacc ggcaatttac
     2161 cgcgtattaa taccgttgaa tctccgtttg ctgaatattc ctcacttgat gaattattcc
     2221 aggaaaccta taaacacgaa caattaatca cccagaaaat taacgaactg gctcatgctg
     2281 caatgaccaa tcaggactac ccaacattta atttcctgca gtggtatgtt tctgagcagc
     2341 atgaagaaga gaaactgttc aaatcgatta ttgataaatt aagcctggca ggcaaaagcg
     2401 gcgaaggtct gtattttatc gacaaagaac tctctaccct cgacacacaa aactaatgct
     2461 ctccggcggc aggtttctgc cgccatttca aatcagtgac ggcaaatctt actctgatcg
     2521 gtcgaaaaac cttcttctga aggtataaat ttgccttttg ctgccagaaa cgctaccagc
     2581 tctcccgctg tcatcccttc tgccgagcaa gtgtgaaaac gtgcctgttc accaaaacgc
     2641 gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc aatcatcata
     2701 tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa agcctagcgt
     2761 agcgattgcc gcttatgaag actttgcgcc agcgcaggac tgaatgcttt ttattgtaca
     2821 tttatgttta caccatatgt aacgtcggtt tgacgaagca gccgttatgc cttaacctgc
     2881 gccgcagata tcactcataa agatcgtcag gacagaagaa agcgtgaaaa acagaaccct
     2941 gggaagtgtt tttatcgtgg cgggaaccac aattggcgca ggcatgctgg caatgccgct
     3001 ggctgcggcc ggtgttggtt ttagcgttac gttaatcttg ttgattgggc tttgggcgtt
     3061 gatgtgctac acggcgctat tactgctgga ggtgtaccag catgttccgg cagataccgg
     3121 tctgggcacg ctggcaaaac gctatctggg acgctacggt caatggctga cgggcttcag
     3181 tatgatgttc ttaatgtatg ctctgactgc ggcatacatc agcggtgccg gtgaattgtt
     3241 ggcctccagc atcagcgact ggacaggtat ttcaatgtcg gcaaccgctg gcgtgttgtt
     3301 gttcactttt gttgccggtg gcgtggtttg tgtcgggaca tcgctggtcg atttatttaa
     3361 ccgttttctg ttcagcgcca aaattatttt tctggtggtg atgctggtat tgctgctacc
     3421 gcatattcac aaagtgaatc ttttaaccct gccgttgcaa caggggctgg cactgtctgc
     3481 aatcccggtg atttttacgt cgtttggttt tcacggtagc gtgccgagta ttgtcagcta
     3541 tatggatggc aacattcgta agctacgctg ggtgtttata accggtagtg cgatccccct
     3601 ggtggcatat attttctggc aggtggcgac gcttggcagc attgattcaa caacctttat
     3661 gggattgctg gctaatcatg ccggattaaa cgggctgtta caggcgttac gcgaaatggt
     3721 ggcctctccg catgttgagc tggcagtgca tttatttgct gatttagccc ttgccacgtc
     3781 atttctcggc gttgcgttag gcttatttga ttatctggca gatttgtttc agcgttcaaa
     3841 taccgttggt ggacgattgc aaactggagc aattaccttt ctgccaccgt tggcgtttgc
     3901 actgttttat ccacgaggat ttgtgatggc gctgggttac gccggtgtgg cgctggcggt
     3961 actggcattg attatccctt cgctgttgac ctggcaaagc agaaagcaca atcctcaagc
     4021 gggttaccgg gtcaaaggtg gtcgtccggc gctggtggtg gtgtttctct gtggtattgc
     4081 tgtgattggc gtgcaatttt tgattgcggc agggttgtta ccagaagtgg ggtgattaga
     4141 tagcctcaaa ttccttattg ggtgccagaa ttaacgctga cacccaattt gacttcttaa
     4201 tgcaggcagc actgcttaaa ttttttacca ctaccgcacg ggcaaggatc gttacgcccc
     4261 ggtttctctt ctgctttgat cggttgctga acagcttttt cctgcggatg cgccatccag
     4321 tacgcatgta gatcaagcgc cgccagtcga atggcatcta cgctctcttc aaacgcttct
     4381 ggcgacatct tctctacccg ctcgaagttt tcctcagtac cgtgcagcgc tatcgcctcc
     4441 agcgctggtt ttaacgaatc gggcaatgtt gaccagtcag aaagtgccac gccccgcata
     4501 tagccaaagc accactcctc aacaatcgtc agctcgctgc catcaacttc tcgcaagccg
     4561 aataacggct caaactgctc cgggaattcg ttcagacgct ctgcggtatc ggccatatgt
     4621 tgaaaagcca ggttcataaa gcgcgtcatc tctttctctg acgcccagcg cggcacatag
     4681 tcagccccac cccacacggc aaccagccac tgtgccggtt caatctcttg cggagaactc
     4741 aacaccgccg tcaataaacc gtccagctcc gccacatcaa ggatggcgtg gtcagtgttg
     4801 tatttggtca gaatatcgtc cagccattcc aactcacttt cgtttaacgg tcccgttttc
     4861 atacgctttt ccttgtggat ctcaactcgc cagcacctat cttacatgcc ggtccgtatc
     4921 agagatactt tttgagtggc tttgccggtg attaaaaatc aaggagggtg taacgacaag
     4981 ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac actgcttatt gctttgattt
     5041 tattcttatc tttcccatgg tac
//
LOCUS       DAZFDC010000214         5043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFDC010000214 DAZFDC010000000
VERSION     DAZFDC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.215"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..384
                     /locus_tag="ACHJ48_004604"
     CDS             <1..384
                     /locus_tag="ACHJ48_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4709998.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRSKGIERQMTEGELKRLVERQLTKWAKHVGNGLSVPPVRRQLAAP
                     KRPPGPTPIELLKQEYERRKAAGFV"
     gene            419..1117
                     /locus_tag="ACHJ48_004605"
     CDS             419..1117
                     /locus_tag="ACHJ48_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4709999.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVTELWKLKRRGEAD
                     NKGSMWWLTSEATEAAPKTTAEMLINAIEQHGPQSADELALMFGITSRRANSSLAMAV
                     SKGRLIRVNQDGKYRYCIPGDNLPAEPKAASVTETDGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQSSFIRKHPDGLILPSLHVANRELRRAKGQVQKWERLCAALREINK
                     HRDVIQKITTGDNG"
     gene            1139..1363
                     /locus_tag="ACHJ48_004606"
     CDS             1139..1363
                     /locus_tag="ACHJ48_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710000.1"
                     /translation="MKIRYHDFGPVSHMLISSTVLETRKHNHILDMLRLADPYLVINT
                     SGIFFLRSTVSGKTSHVLRAYKTAVREEGE"
     gene            1360..1716
                     /locus_tag="ACHJ48_004607"
     CDS             1360..1716
                     /locus_tag="ACHJ48_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like protein"
                     /protein_id="HFO4710001.1"
                     /translation="MSEINYQELRVELGAAKKRIEELEANRVVLEVENERLKHAMAVA
                     LEHISVTDAGQAGVAAMIIYDAMYHSEKTDPYAFLAELRAQGVEMVREHPAIKLCSLT
                     HICDELVAELRKGGNK"
     gene            1749..1901
                     /locus_tag="ACHJ48_004608"
     CDS             1749..1901
                     /locus_tag="ACHJ48_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710002.1"
                     /translation="MAKKHHYFAEGEITSICGGWMYFGNEREPDTFESPDDCKKCRRK
                     LNKGGK"
     gene            1898..2209
                     /locus_tag="ACHJ48_004609"
     CDS             1898..2209
                     /locus_tag="ACHJ48_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710003.1"
                     /translation="MSVIKEMPVERNEYGCWTHPEYEKFCDGREYISTEEFNAWMEKN
                     NLQWAIRSMDEDDFNLDADGPDISAWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2336..2899
                     /locus_tag="ACHJ48_004610"
     CDS             2336..2899
                     /locus_tag="ACHJ48_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710004.1"
                     /translation="MTELTKEWLQNTITGIESSRDEIPFGLDEDQNNMLTALKIALAS
                     LASVSDERAAYELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGN
                     FRENKGSSTNNFRIISETSTNSPAIPDEVLSAILKVARLRADFDASEVDRRGIGSCLD
                     EAEQELIVTINEYASQIAVEATQGENQ"
     gene            3009..3113
                     /locus_tag="ACHJ48_004611"
     CDS             3009..3113
                     /locus_tag="ACHJ48_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710005.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMERLGN"
     gene            3357..3512
                     /gene="hokD"
                     /locus_tag="ACHJ48_004612"
     CDS             3357..3512
                     /gene="hokD"
                     /locus_tag="ACHJ48_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4710006.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3680..3958
                     /locus_tag="ACHJ48_004613"
     CDS             3680..3958
                     /locus_tag="ACHJ48_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710007.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3960..5009
                     /locus_tag="ACHJ48_004614"
     CDS             3960..5009
                     /locus_tag="ACHJ48_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4710008.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRCRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
BASE COUNT         1355 a         1132 c         1407 g         1149 t
ORIGIN      
        1 ctggctgagt ttgtggcgtt gatttcggag agcggggcaa atccatttgg cctgacggtg
       61 gatgccgtga tggaggagta ccgtcgctgg aggaatgaat cctggcggta cgacgggagt
      121 gataaatacc cgtggccaca gccggtgctg taccacattt gcctcgagat gcgttcaaag
      181 gggattgagc gccagatgac cgaaggggag ttaaaacggc ttgtggaaag gcagctgacg
      241 aaatgggcaa agcatgttgg taatggcctc agtgttccgc cagtccggcg acaactggca
      301 gcacccaaac gcccgccagg gccaacgcca attgaattgc tgaaacagga atatgagcgc
      361 cggaaggcgg ccggttttgt ctgagttgag aagtaatttt taccgggagg aaaatttaat
      421 ggaaaccgtt tttgacgcac tgaaagcact gaaaaaagcc tcgtcgcacg agattgcagc
      481 ccgtcttgaa atcagccgtg acgatgctgt taccgaactc tggaagctga agcgtcgtgg
      541 cgaagctgat aacaaggggt cgatgtggtg gctgacgagt gaggcaactg aagcggcccc
      601 aaaaaccact gcagagatgc tgattaacgc gattgaacag catggtcctc agtcggctga
      661 cgaactggcg ttaatgttcg ggattacctc ccgccgggcg aattcatcac tggcgatggc
      721 tgtcagcaaa ggtcgtctga ttcgcgtaaa tcaggacggt aaatatcgtt actgcatacc
      781 gggcgataat ttaccggcag agccgaaagc tgcatcggta acggaaaccg atggtaaagc
      841 ctttcctcag ccggcaggtg ttgcgttacc agtccgggaa gcggaaacac aggaagaaat
      901 taaaactgaa agtgtggcgg tcacagtgca gtcacagtcg tcgttcatca gaaagcatcc
      961 ggatggtctg attttaccat cgctgcatgt ggctaaccgc gagctgcgcc gggcaaaagg
     1021 tcaggttcag aagtgggagc gcttatgcgc cgcgctgcgg gaaattaata agcatcgtga
     1081 tgtcatacag aaaataacga caggagataa cgggtaattt ttacagcagg gtgatgatat
     1141 gaaaatcaga tatcatgatt tcggccccgt gtcacatatg cttatttcaa gtacggtgct
     1201 ggaaacaaga aaacataatc acatactgga tatgctgcgt cttgctgacc cgtatctggt
     1261 aataaacacc agtggaattt tctttctgag aagcactgtg tcaggaaaaa catcgcatgt
     1321 gcttcgggca tataaaacag cagtgcggga ggaaggagaa tgagcgagat taactatcag
     1381 gagctgcgtg ttgagctggg agcagcaaaa aaacgcatag aagagctgga ggctaatcgt
     1441 gtggtgcttg aggtggaaaa tgagaggttg aaacacgcaa tggccgtagc ccttgagcat
     1501 atatccgtca cagatgcagg acaggcaggt gtcgctgcaa tgattatcta tgatgccatg
     1561 taccacagtg aaaaaactga cccctacgct tttctggcag aattgcgggc gcagggtgtg
     1621 gagatggtgc gcgaacatcc agcaatcaaa ctttgctctt tgacgcacat atgtgacgag
     1681 ttagtcgcag agcttcgcaa aggaggcaac aagtgagtaa aacgaatccg ggctgggcaa
     1741 ggccacttat ggcgaaaaaa catcattatt ttgctgaagg tgaaataaca agtatttgcg
     1801 gtgggtggat gtattttggc aatgagcgtg agccggatac attcgaaagt ccagatgatt
     1861 gtaaaaagtg tcgtagaaaa ttaaataagg ggggtaaatg agcgtaatta aagaaatgcc
     1921 agtggaacgt aatgaatatg gctgctggac gcatcctgaa tatgaaaaat tctgtgatgg
     1981 tcgggaatat atttcaacgg aagagtttaa cgcctggatg gagaaaaata atcttcagtg
     2041 ggctatccgt agtatggatg aagatgattt taatctggac gcagatggcc ctgatatttc
     2101 agcatgggaa cctgagcgac cagaaggtga gggatggttt atcggttcga tacatgacac
     2161 cgaagatggt cctgtttgtg tatggctgag aaataaggcc gaagcataaa ggctataaac
     2221 caacaactaa acactgaaaa tttaaatcag aaatgatttt tattaaatcc ttaaccggag
     2281 ggattcctgc accctcagaa catcaggaga ccgcccgaaa gggcggtagt gaaaaatgac
     2341 tgaattaacc aaagaatggc tacagaacac gattaccgga attgagtcat cacgggatga
     2401 aataccgttc ggactcgatg aagatcaaaa caacatgctt accgcattaa aaattgcact
     2461 ggcatcactg gcatcagtat cggatgaacg ggcagcctat gaattattta tggagaagcg
     2521 tttcggggaa tctgtagatc gccgcagagc aaaaaatggc gatagagaat acatggcatg
     2581 ggatatggcg cttggctgga ttatctggtg tcaccgcgcc gccatgcttc aggctggaaa
     2641 ctttcgggaa aataagggtt cgtcaaccaa taattttcgg ataatctcgg aaacgtcaac
     2701 caactctccg gcaatccctg atgaggtgtt gtccgcaatc ctgaaggtcg ccaggcttcg
     2761 tgcagatttc gatgcttctg aagtggacag gcgaggtatc ggtagttgtc tggatgaggc
     2821 cgagcaagaa cttatcgtta ccattaacga atacgccagt cagatcgcag tagaagcgac
     2881 acagggggag aaccaatgag ccggccagat gcatttgcag gcgttgctat agctattgct
     2941 tttctagtat atgttatttg tcggtggggg taaaaacgtt cgccgggatt cacaccaagg
     3001 gagggaatat gtcggatgat atttcactgg taatggaagg cgctctggct gttattgctg
     3061 ttgtgggtgt ttactgcctg gttgtgtttt tgatggagcg cctggggaac tgaattcatt
     3121 ccgtatggga attcccatat cgggcaaaac ggtttgctgt aaagcgagag ttaagtagaa
     3181 ttgctgcggg tgcttgaggc tgtctgcctc gggcatgcca ccgtaaggca gacagagaaa
     3241 agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta
     3301 tgcttcacaa acgtaggtta gcctcttacg cgccgaaagg caaggagaag caggttatga
     3361 agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg
     3421 cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg
     3481 ctgtcttcac agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc
     3541 caccgctgat gtgtcaggca tcctcaacgc acccgcactt aacccgcttc ggcgggtttt
     3601 gttttttctg gtcgttctgg tttacaatcc atccgtcagc ctgaacaact ggcacctgct
     3661 gcgccagcag agaaaacaga tggcgcacga taccaaattt tacaattcgg ataactctgc
     3721 cgcccctgcc agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggtatcagca
     3781 cgacccctgc accgaagaac aggctgaatg gctgattcag tgttaccgca ggcgcggata
     3841 cgaggttaag aaagccctca gtctcgatta tcgtcactgg ataatctccg tcaggcttcc
     3901 ttactccgaa cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg
     3961 tgcgggtatt acttcgacct gttctggtac cggaactcgg gctggtgatc gttaagccgg
     4021 gccgtgaatc catgccggta ttccacaata cccgggtact ggtggagccg gaaccgaaaa
     4081 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgttcg ccagccgctg gcggaggata
     4141 aatcattact gccatttttc agcgacgaac gagtgattcg tgctgctggt ggcgctggcg
     4201 cactgtctga ctggttactg cgccatgtta aatcctgcca gtggccacac ggcgattatc
     4261 accacagtga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     4321 gcgacaacca gttgcgtgac cagacctccg aatcacttga gcaacttgct caccaaaacc
     4381 tgtcagcatg gatgattgac gtcattcgtc acgcaatgaa tggcacacag gagcgtgaat
     4441 tatcgctggc tgaattatcc tggtgggcgg cctgcaatca ggtggtggat gcactacctg
     4501 aggcagtagc gcgtcgttcg ctgggattac cagcggaaaa aatccgctcc gtataccgtg
     4561 agagtgacat cgtaccggga gaacagacag ccatcagcat actgaagcag cgcacaaaaa
     4621 atattgcgct gccacttcac gtccaccagc aacaaaatcc accacagaaa aaaacggttg
     4681 tcagtatcgc cgttgatccg gagtctcctg aatcgttcat gaggcggcct aaacgttgcc
     4741 gctgggttaa tgagaaatac acgcgctggg taaagacaca gccgtgtgcg tgttgtggta
     4801 agcctgctga cgatccgcat cacctgattg gtcatggtca ggggggaatg gggacaaagg
     4861 cccacgatat tttcacgcta ccgttgtgcc gggagcacca caacgaactt catgcagacc
     4921 cgctggagtt tgagaaaaag tacggctctc agattgagtt aatttttcgt tttcttgatc
     4981 acgcctttgc gactggcgtg ctcgggtaaa agaggtgact gatgctcata gatttggttt
     5041 tac
//
LOCUS       DAZFDC010000215         4911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFDC010000215 DAZFDC010000000
VERSION     DAZFDC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.216"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            155..421
                     /locus_tag="ACHJ48_004615"
     CDS             155..421
                     /locus_tag="ACHJ48_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4710009.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            418..1008
                     /locus_tag="ACHJ48_004616"
     CDS             418..1008
                     /locus_tag="ACHJ48_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO4710010.1"
                     /translation="MMMPALQKVPFSGLPFSGICGYSFSAAAKSAAGRRNPCNPKATY
                     DAPCVFFCVAINATERQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVFFEA
                     GIPTPVWAIAIERRNSGDSSYLLLEGDCLMATTLTPSHPEFVFVFAAVRRADRHPRIC
                     MLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            1001..1288
                     /locus_tag="ACHJ48_004617"
     CDS             1001..1288
                     /locus_tag="ACHJ48_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFO4710011.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            1281..1736
                     /locus_tag="ACHJ48_004618"
     CDS             1281..1736
                     /locus_tag="ACHJ48_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710012.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPEIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWRKKYLDIYPQDPVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            1872..2192
                     /locus_tag="ACHJ48_004619"
     CDS             1872..2192
                     /locus_tag="ACHJ48_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFO4710013.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            2207..4540
                     /locus_tag="ACHJ48_004620"
     CDS             2207..4540
                     /locus_tag="ACHJ48_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO4710014.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAATKVAAVTGSLPPADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTCPGTGNTYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
BASE COUNT         1081 a         1436 c         1370 g         1024 t
ORIGIN      
        1 ttccagtccg gatggataaa aaacacacag ccattgtaag gttgccggaa caaattcccc
       61 ctgttgcgtc tgctgaaaat attcacaaaa taaagcgcta cccgaagccg gacggactta
      121 tccggtgctg tatggacatt aacgaggtag cccgatgcaa gctgtttttt cttccccgtc
      181 tcccgcccct gtgacgccac tgatgccgct gccggacatc acgcaggagc gttttttacg
      241 tctgccggaa gtgatgcacc tgtgcggcct gtcacgctcg accatctatg aactcatccg
      301 taagggggaa tttccgccgc aggtgagtct tggcggtaaa aatgtggcct ggctgcactc
      361 tgaagtcacc gcatggatgg ccggacgcat tgccggacgc aaacgggggt acgacgcatg
      421 atgatgcccg ctctgcaaaa agtccctttt tctggcttgc ctttttccgg catttgcgga
      481 tatagttttt ccgctgccgc aaaatcggca gccgggcgta ggaacccgtg taacccgaag
      541 gcgacatatg acgcgccatg cgtctttttt tgtgtcgcaa tcaacgccac agagcgccag
      601 attatggtgt ggcgtgtggt ttgccgtgca ggtatgatcc tgttcgcaat cgcatgttat
      661 gccactgagt caatggtagc tcaggcgggg cagcctccgg gctggccggt attcttcgag
      721 gccggtattc ctacccccgt ctgggctatc gccatcgagc gtaggaactc cggcgatagc
      781 agttatttgc tactcgaagg agactgcctt atggctacaa cccttacccc ttcacaccct
      841 gaatttgtct ttgtgtttgc ggctgtccgt cgcgcagacc gtcatccccg tatctgcatg
      901 cttcgcaccg tcgccggtga tgaacgcagc gcccgccgtt cccttgtccg tgactatgtg
      961 ctctcccttg ctgcccgtct gccggtggtg gaggtgtccc gtgcgtaata aaaaagcccc
     1021 tcagaccgtc tcagcacgtc atgacgcccg tgaacacctc agcattgagg cttaccataa
     1081 gctcaaccgc gccagcgccg tatcccagtt tgttgggggt gatttgattc accgtgaact
     1141 ctccggcctg catcagctct acattccgca tattttcagc tacctgaatg aagatattga
     1201 ttttgtgctg aatgagctga aagccaaagg cctgtgccgc gattttctcg cccagcagaa
     1261 agaccgggga gacaggacgc atgtttgatt ttccccagcc cggtgagatt taccgttctg
     1321 ccggttttcc cgatgtggcc gtggtcggca ttctggaaga cggtattccg tgggaaatgc
     1381 cgtaccgctg cccggaaatt gtctggaacc cgtaccgccg taaattcagt atccttgtgc
     1441 gtatcctcgc tgacgggcgc accacagaca tcccgctggg gcgttttctg cgggaattta
     1501 cctgtgaccg tcctgacctg tttaaacgca gccccgtaaa ccggcatgcg gtactgaaag
     1561 aaatggccgg agacccggaa ttacagaaat ggcggaagaa atatctggat atttacccgc
     1621 aggaccctgt tccggtcagc cgggcggcac cggtggcgcg ggaatggcgg gaaattcccc
     1681 gcacagagcc tgacccggaa atcaccccgg ataacagtta ccgcaattat ctgtaattaa
     1741 aaaacgacac ccgaaaaatt aaatgtgcgt attcgcgcag ggatacgcac gtcttcagga
     1801 gacgcagata tgccctatca gttaatgcaa ccggcacgga atgcagtcat ctgtcacagg
     1861 gaggaaagca aatgaaaaca cccttaccgc ccgtcttacg cgcagccctt taccgtcgtg
     1921 ctgtcgcctg tgcctggctg accgtgtgcg aacgtcagca ccgctacccg catctcaccc
     1981 ttgagtcact ggaggcggcc atcgccgctg agctggaagg tttttatctg cgccagcacg
     2041 gtgaggaaaa agggcgtcag atagcctgtg ccctgctgga agatttaatg gaatccggcc
     2101 ccctgaaggc cgcgccgtcg ctgtcctttc tcgggctggt tgtgatggat gaactctgtg
     2161 cccgtcacat aaaagcgccg gtactgcact gaaggagaac aacaccatga aaatgaacgt
     2221 aaccgccacc gtcagccatg cgctcggcca ctggccgcgt attctcccgg cgctggggat
     2281 tcaggtgctg aaaaaccgtc atcagccctg tccggtctgt ggcgggagtg accgcttccg
     2341 ttttgatgac agggaggggc gcggcacctg gtactgcaat cagtgtggtg ccggtgacgg
     2401 cctgaaactg gttgaaaagg tgtttggtgt ctccccgtcc gacgcggcca caaaggtggc
     2461 tgccgtgacc ggcagcctgc ccccggctga cccggcagtg acggccgccg ccggtgctga
     2521 aacagacgct gcccggaaga acgccgccgc actggcacaa accctgatgg cgaaaacctg
     2581 tcccggaacc ggtaacacct acctgacccg caagggcttt cccggccggg aatgccggat
     2641 gctgaccggc acacacagag ccggtggcgt gagctggcgc gccggtgacc ttgtggtgcc
     2701 actgtatgac gacagcggcg aactggttaa ccttcagtta atcagtgctg acggccgtaa
     2761 gcgcaccctg aaaggcggac aggtcagggg cacctgtcac acccttgaag gacagaatca
     2821 ggccggaaaa cgtctgtgga tagcggaggg atacgcgacc gcacttaccg tgcatcacct
     2881 gaccggtgaa acggtgatgg tggcgctttc ttccgtgaac ctcctttctc tggccagcct
     2941 tgcccggcag aagcatccgg cctgtcagat tgtccttgcc gcagaccgtg acctcagcgg
     3001 tgacggccag aaaaaagccg ccgcagccgc agatgcgtgt gaaggtgttg ttgccctgcc
     3061 gccggtcttc ggtgactgga atgatgcctt cacgcagtac ggcggggagg ccacccgtaa
     3121 ggccatttac gatgccatcc ggccaccggc tgaaagcccg ttcgacacca tgagcgaagc
     3181 agagttttcc gccatgagta ccagcgaaaa ggccatgcgt atctatgagc attacggcga
     3241 ggcgctcgcg gtcgatgcca acggccagct tctgtcccgc tatgaaaatg gtgtctggaa
     3301 ggtgctgcca ccacaggact ttgcccggga tgtggccggg ctgtttcagc gtctgcgcgc
     3361 gccgttctcc tccgggaagg tggcctccgt ggtggacacc ctgaagctga ttattccgca
     3421 gcaggaagcc ccctcccgcc gcctgattgg ctttcgtaac ggcgtgctcg acacgcagaa
     3481 cggcacgttc cacccgcaca gtccgtcaca ctggatgcgc accctgtgcg atgtggattt
     3541 caccccgccg gtggacggtg aaacactgga gacccacgcc cccgcgttct ggcgctggct
     3601 tgaccgtgcc gccggtggcc gtgcggaaaa acgcgacgtg attctggccg cactgtttat
     3661 ggtgctggca aaccgctacg actggcagct ctttctggag gtgaccggtc ccggcggcag
     3721 cggcaaaagt atcatggccg aaatcgccac cctgctggcc ggggaggata acgccacgtc
     3781 ggccaccatc gagacgctgg aatccccgcg tgaacgtgcc gcgttaactg gcttctcact
     3841 gatacgcctg ccggaccagg aaaaatggag cggcgacgga gccggactca aggccatcac
     3901 cggcggcgat gcagtgtccg ttgacccgaa ataccgggat gcgtactcca cgcacatccc
     3961 ggcggtgatt ctggccgtga acaataaccc gatgcgcttc accgaccgca gcggaggcgt
     4021 gtcacgccgg cgggtgatta ttcacttccc ggaacagata gccccgcagg agcgcgaccc
     4081 gcagcttaag gacaaaatca cccgcgagct ggcggtcatc gtgcgtcacc tgatgcagaa
     4141 gttcagcgac ccgatgctcg cccggtcact gcttcagtcc cagcagaact cagacgaggc
     4201 actgaacatc aaacgggatg ccgacccgac gtttgatttt atcggctatc tggaaaccct
     4261 gccgcagacc agcggcatgt atatggggaa cgccagtatc atcccgcgta attaccgtaa
     4321 atacctctat cacgcctatc tggcctacat ggaggcaaac ggctaccgga atgtactcag
     4381 tctgaaaatg ttcgggctgg ggctgccggt gatgctgaag gaatacggac tgaattacga
     4441 gaagcgccat accaaacagg ggatacagac caacctgacg ctgaaagagg aaagctacgg
     4501 cgactggctg ccaaaatgtg acgaccctgc aacaacctga ccatcatgac tcatctgacc
     4561 ggcatctgcc ggtctttttt tatccctgaa ttccccgaag gtgaacaatc cactgttcac
     4621 ccttcaccgt atattcaccc gttatcgcac tgaaattaaa aaagaaaaac gaaaggtgaa
     4681 cagtgtgaac aatcaaatca aaaaaaactt tttttctccc tgtgtgattt cagtacgggg
     4741 gattaatcac cggtatgagt cacaccggca gaatgccgga ggtgaagaat cgaatgttca
     4801 cccttcaccc attattcacc acctatcata ctgaaataaa aggagaaaaa agaaaggtga
     4861 acagtgtgaa cagttctttc gaaaaaaaat ttttttcctg agttaacgtg c
//
LOCUS       DAZFDC010000216         4896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFDC010000216 DAZFDC010000000
VERSION     DAZFDC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.217"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            60..536
                     /locus_tag="ACHJ48_004621"
     CDS             60..536
                     /locus_tag="ACHJ48_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4710015.1"
                     /translation="MDGELKNMKLNINQLASLSGLHRQTVTARMADVPLAPGSNEKKK
                     LYFLTDLITSLLEKPPSTEDEEMNPHDRKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQILETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIACCDEADM"
     gene            533..2656
                     /locus_tag="ACHJ48_004622"
     CDS             533..2656
                     /locus_tag="ACHJ48_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4710016.1"
                     /translation="MNPVNECHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVCKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPVSRRCVPDGVRFIEATVDVQGGKNSRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCGPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRQCRREGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFFDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVGPEGRSDATTPTASSGRKEK
                     CRHRQEKLRTERAEDNPWVTSGGWL"
     gene            2653..2865
                     /locus_tag="ACHJ48_004623"
     CDS             2653..2865
                     /locus_tag="ACHJ48_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710017.1"
                     /translation="MSIDDVRNMIQRYRDAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLTAMVNRRRGEPGFKLARF"
     gene            2865..4370
                     /locus_tag="ACHJ48_004624"
     CDS             2865..4370
                     /locus_tag="ACHJ48_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4710018.1"
                     /translation="MAISDDVIGFFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGAKGITVEPNPVMKNGEI
                     AAELAADIRRLWAEWSVSPDVTGQYTRPMLERLLLRTWLRDGEVFAQMVSGAANGLKR
                     SAGVPFWLEAMEPDFVPMRSDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGIQSDTKE
                     IAADKMVHLKFTRRLHQTRGASMLSGVMVRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYDDQGADKKEEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLENFRNGQLRAV
                     AAGSRLSFSSTARNYNGTYSSQRQELVESTDGYLILQDCFIGAVTRPVYRAWLNMVVA
                     AGLLKIPSCLDMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRH
                     PDEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQRQQATDSQHEE"
     gene            4360..>4896
                     /locus_tag="ACHJ48_004625"
     CDS             4360..>4896
                     /locus_tag="ACHJ48_004625"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFO4710019.1"
                     /translation="MKNKSWFRMQAGEPGDADIYIYDEIGFWGVTAKQFVCDLNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNEGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAV"
BASE COUNT         1119 a         1159 c         1540 g         1078 t
ORIGIN      
        1 tttctttaca aaaaaaacag aatcacttct gttcagaggg gtatgtgggg gacgcgtaga
       61 tggacggcga gctgaaaaat atgaagttaa atattaatca actggcatcc ctttcaggcc
      121 tgcaccggca gactgttacc gcaagaatgg cagatgttcc tcttgcacca ggcagcaatg
      181 agaagaaaaa actttatttc ctgacggatt tgattacctc gctgctggaa aagccaccat
      241 ccaccgaaga tgaggagatg aatccacacg atcgcaaggc atggtatcag tccgagcgcg
      301 agcgccttaa atttcagcat gaaactgttc agcttgtgcc cgtcagtgat gtcaggcggt
      361 cgttttccgt cgtggtgaaa gcgattgttc agatactgga aacatggcct gacaggctgg
      421 agagggacag ggggtggact gcatcacaac tgaacgaagt gcagatcgtt gttgatgaga
      481 tccgtgacac gctggaaaag gcagtgattg cctgctgtga tgaggctgat atgtgaatcc
      541 ggttaacgag tgccatagcc gcgcatccga tatctggcgc gaagtggcct cgctgtttcg
      601 cccacctggc cggttacctg tggcggaagc catcaggcgt tatatgcggg taccacgggg
      661 agccaatact tccggcccgt gggagtcatc tctgacgccc tatatgattg accccattaa
      721 tacattatca gcccgtgaat atgacgcggt ggtgtttgtg ggaccggcgc gaaccgggaa
      781 aaccgaaggg ctgattgatg gctggattgt gtacggcatc atctgtgatc cggcggatat
      841 gctggttgtg cagatgaccg agaccaaggc gcgtgagcat tccagaacgc gtctttccag
      901 gacgtttcgt cacagcccgg aggtctgcaa acgcctcagc ccttcccgta atgacaacaa
      961 cgtccacgat aaaatgtttc ttgacggctc cttcctgaag attggctggc cgtcgatcac
     1021 cgtattttcc tcttcggatt accgtcgtgt ggcgctgacg gattatgacc gttttcctga
     1081 gaacgttgac ggtgaagggg atgccttcac cctggcatca aagcgtacca ccacctttat
     1141 gtcctcaggg atgaccctgg tcgaaagttc acccgggcgg gatatcactg acaccaaatg
     1201 gcgatgtggt ggtgcacatg aggctccgcc aacaacgggg atcctgtcgc tgtataaccg
     1261 gggagaccgc cgccggtggt actggccgtg tccgcactgc ggggaatatt ttcagccggt
     1321 gatggataac atgaccggtt accggaataa cccagatttt gtggctgccg gtcaggctgc
     1381 ccgtctgatg tgtccacact gtcgcgggct gattgccccg gagcagaaac gcgaactgaa
     1441 taaccagggg atctggcttc gtgaaggtga gcgggcggcg gcggacggca gtatcaccgg
     1501 aacgccacga aattccagga ttgcgagctt ctggatggag ggtccggctg ccgcgtttca
     1561 gacctgggaa cagctgattt ttaagctgct ggcggcagaa gaagagtatg agcgaaccgg
     1621 cagtgaagaa acgctgaaag cggtggtgaa caccgatatc ggacgcccct atctgccccg
     1681 ttcagctacg gaacagcgta aaagtgaact gcttgaacag cgtgcggagc cggtttcccg
     1741 tcggtgtgtg ccggatggtg tgcgttttat tgaggcaacg gttgacgtac agggcggtaa
     1801 aaacagccgt tttgtggtgc agatcaccgg atacggagaa cagggagaac gctggattgt
     1861 tgatcgctac aacatccggc attcacttcg ctgtggtccc aacggtgaaa gtcttcctgt
     1921 cgatccggca gcgtatccgg aagactggga cttattgctg acggatgtgt ttcataaaac
     1981 ctggccgctg gcgtctgatc cggatgtccg tatgcgtctt atggcgatgg ccgtggatac
     2041 gggcggtgaa gccggggtga cggataacgc ctatcgtttc tggcggcagt gccgccgcga
     2101 agggctgggt aaccgggtgt ttctgttcaa gggcgatggc ctgcgtcgcg acagactgat
     2161 taaccgtacc ttcccggata acaccggcag aagtgcccgc cgtgccaggg ccagtggtga
     2221 tgttgccctg tggctggtgc agaccgatgc gtttaaggat cgggtgaata atgcgctgtg
     2281 gcgtgacacg ccgggaccga attatatcca ctttcctgac tggctggggc ggtggttttt
     2341 cgatgagctg acctatgagg agcgcggcag tgacggaaaa tggcgaaagc cgggcagggg
     2401 cgctaacgaa gcatttgacc tgctggtcta cgcggatgcg cttgccgttc tgcatggcta
     2461 cgaaaagatc cgctggccgt cagcacctga ctgggcacaa cgggaaacgt ggcttgtcgg
     2521 tccggagggg cgttcagatg cgacaacgcc cacggcgtcg tcaggaagga aagaaaaatg
     2581 ccgtcaccgg caggaaaaac tgcgcacaga gcgggcagag gataatccgt gggtaacatc
     2641 aggaggctgg ttgtgagtat tgatgatgtc aggaacatga tacagcggta tcgcgatgcg
     2701 gaaatggcgg tactggaggg aaaatctgtc acgtttaacg ggcaacaact gacgctggaa
     2761 agtctgtcac agatccgcgc cggtcgtcag gagtgggagc gaagacttac ggcgatggtg
     2821 aaccgcagac gcggggagcc cgggtttaaa ctggcgaggt tctgatggcg attagcgatg
     2881 atgtgattgg ttttttctcg cccggctgga aagcagcgcg attacgctca agggcgttaa
     2941 ttatggccta tgaggccgtg aaacccacca ggacgcataa agcacgccgg gaaaaccgtt
     3001 ctgccgatca gctcagtaaa tacggtgcgg tttcccttcg ggagcaggcc cgctttctgg
     3061 atatcaatca tgacctggtg ataggcgtgt ttgacaagct ggaggagcgg gtgattggtg
     3121 cgaaggggat caccgttgag cctaatccgg tgatgaaaaa cggggagatt gcggcggagc
     3181 tggcagcgga tatccggcgg ctgtgggcag aatggtctgt cagtccggat gtgaccggac
     3241 agtacacccg tcccatgctc gaacgcctgt tactgcgtac ctggctgcgt gacggggagg
     3301 tgtttgcgca gatggtcagt ggtgcggcaa acggtcttaa gcggtctgcc ggtgtgcctt
     3361 tctggcttga ggcgatggag ccggattttg tgcccatgcg cagtgatgag tctgccggac
     3421 tgaatcaggg cgtttttctg gatgagtggg ggaggccgaa aaaatatctg gtttacaaaa
     3481 attatccggt ttccgggata cagagtgata ccaaagagat cgccgcagac aaaatggtgc
     3541 atctgaaatt cacccgccgc ctgcaccaga cgcgtggtgc gtccatgttg tccggtgtga
     3601 tggtgcgtat cagtgcgctt aaggaatatg aagatgccga actgacagcg gcgcgtattg
     3661 ctgcggcgct ggggctgtat atccgtaagg gggatggaca ggattacgac gatcagggag
     3721 cagacaaaaa agaagagcgg gaagtgcata tcaccccggg cattatctat gacgatttgc
     3781 gcaagggtga ggatatcggc atggtcaaat ccgaccgtcc taatccaaat cttgaaaatt
     3841 ttcgtaatgg tcagttgcgt gcggtggctg caggcagtcg tctgagcttt tccagtaccg
     3901 cacgcaacta caatggcacc tacagttcac agcggcagga gctggtcgag tccaccgacg
     3961 gttaccttat cctgcaggac tgctttattg gcgcggtaac ccgcccggtg taccgtgcat
     4021 ggctgaatat ggtggttgct gccggtctgc tgaaaatccc gtcttgcctg gatatgaaga
     4081 cgttatataa cgcgacatat tcaggtccgg tgatgccgtg gatagatccg gttaaggagg
     4141 ctgaagcctg gcggatacag atccggggtg gtgccgcaac ggaatccgac tgggtacgtg
     4201 ccggcggacg tcatccggat gaggttaaac gtcgccgcaa ggctgaaatt gatgaaaaca
     4261 gcagactggg gctggtcttt gatactgacc ccgtcaacga caaaggaggc aacagtgccg
     4321 gaactgaacg acagcgtcaa caggccaccg acagccagca tgaagaataa atcctggttc
     4381 aggatgcagg ctggggagcc gggtgacgcg gatatttata tttatgacga gattggtttc
     4441 tggggagtta ccgcgaagca gtttgtctgc gatctgaatg ctctgggtga tatcacccac
     4501 attaatctcc atatcaactc accgggtggc gatgtctttg aaggcatcgc catttttaat
     4561 gccctgaaaa atgagggggc gaccattacc gtgtatgtgg atggcgttgc cgcatcgatg
     4621 gcatctgtga ttgcgatggc cggtgatacg gtcattatgc cggaaaatgc tttcatgatg
     4681 atccataagc catggggttt cagtgggggg gatgctgagg atatgcgcag ttatgccgat
     4741 ctgctggata aagtcgaatc ggtactgttg ccagcctatg cgcaaaaaac aggaaaaacc
     4801 accgatgaaa ttgccgccat gctggcggat gagacctgga tgtcaggtgc cgagtgtctg
     4861 gcacacggat ttgctgacca ggtgacgcca gccgtt
//
LOCUS       DAZFDC010000217         4774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFDC010000217 DAZFDC010000000
VERSION     DAZFDC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.218"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(104..174)
                     /locus_tag="ACHJ48_004626"
     tRNA            complement(104..174)
                     /locus_tag="ACHJ48_004626"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Met,seq:cat)
     gene            complement(319..873)
                     /locus_tag="ACHJ48_004627"
     CDS             complement(319..873)
                     /locus_tag="ACHJ48_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4710020.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGVTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(870..1160)
                     /locus_tag="ACHJ48_004628"
     CDS             complement(870..1160)
                     /locus_tag="ACHJ48_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO4710021.1"
                     /translation="MVDLRKAARGQMCQVRIPGYCNHNPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTGDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1160..1759)
                     /locus_tag="ACHJ48_004629"
     CDS             complement(1160..1759)
                     /locus_tag="ACHJ48_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO4710022.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLYQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPIGGAITPREREMVFGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTQDMALLKSFDAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKAVLNVLWNWILFRKFSSLEEVENVAAQLLEFA"
     gene            complement(1915..1992)
                     /locus_tag="ACHJ48_004630"
                     /pseudo
     CDS             complement(1915..1992)
                     /locus_tag="ACHJ48_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2245..2400)
                     /gene="hokD"
                     /locus_tag="ACHJ48_004631"
     CDS             complement(2245..2400)
                     /gene="hokD"
                     /locus_tag="ACHJ48_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4710023.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(2644..2748)
                     /locus_tag="ACHJ48_004632"
     CDS             complement(2644..2748)
                     /locus_tag="ACHJ48_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710024.1"
                     /translation="MSDDISLVMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(2858..3136)
                     /locus_tag="ACHJ48_004633"
                     /pseudo
     CDS             complement(2858..3136)
                     /locus_tag="ACHJ48_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(3727..3939)
                     /locus_tag="ACHJ48_004634"
     CDS             complement(3727..3939)
                     /locus_tag="ACHJ48_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710025.1"
                     /translation="MSNAQKVINAEKYNEWVKKFSEQIFKITGDENAAKNELEPWTPE
                     GVDPNYCWWDVDPVDAANEAMSYHND"
     gene            complement(3988..4344)
                     /locus_tag="ACHJ48_004635"
     CDS             complement(3988..4344)
                     /locus_tag="ACHJ48_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710026.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRILRQEERERRRNIEKLIQIE
                     RNVKARIRRVTKSRTE"
     gene            complement(4402..>4774)
                     /locus_tag="ACHJ48_004636"
     CDS             complement(4402..>4774)
                     /locus_tag="ACHJ48_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4710027.1"
                     /translation="EEREEVKARIVEFVRPSGRETFRQQADKTGVSKTAIRRLSGALA
                     ASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQNIICRECR
                     QSEVMQRVLAFYQGNFQEAVL"
BASE COUNT         1165 a         1277 c         1002 g         1330 t
ORIGIN      
        1 agtttctttt aacaaactcc ccgcaaacca gacaacagtc aaccgcctga attgtgagac
       61 atttaaaaaa aggcccgcaa aagcgagcca gggaaaataa gtgtggcgcg ttgtactgga
      121 ttcgaaccag cgacctggcg attatgcgtc gctcgctctc accactgagc taaagggccg
      181 ggagcagaat aataacggtc cgtaattaat tccgcaataa aaaacccgct caatggcggg
      241 ttctggtaaa gttcatgcgc ttggttcgcc tcgcgataca gctttgcgaa gcataccgga
      301 attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa aagccataac
      361 cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc gtctttcaca
      421 ggttcttaaa ctccattccg gatgtgcatc attcagcagt tcagccattt tgcgcttagt
      481 caccccccgc cccacataac gctgactcag aacattgagc agcccgggat aacctgccag
      541 tacttcacca ataaccctgt cgattattaa cgcctctgaa tcggtacaat gtgccagcca
      601 gcttttttga ttgccgttga tcatatcccg caaaaaagcc tcaagttcag gtttgtccag
      661 acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca gctttttaga
      721 ggtcagcaac tggttaaaca tatttcccgt cttaccgccg ccaatatatg accagcgccc
      781 ccacatgcgc agttttccct gaatccagac actttccagc gtggtgagac gaaggtgttc
      841 tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct ccagatttct
      901 tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt gtaatcgccg
      961 gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc tgcctgcata
     1021 tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc cagcacagac
     1081 gtttcgggat tgtgattgca gtagccaggg attctgacct ggcacatctg gccccgcgct
     1141 gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca ttttcgactt
     1201 cctccaaaga ggagaattta cggaacagga tccagttcca caggacgtta agtacggctt
     1261 tataaacctg ttgaaattca gtttcgtcca tattcgcaaa agcaatggac ttcgcccgac
     1321 gcccgcggct accgtctgga taaaaatgct cggtgtagaa gccggcctga atggtcaccc
     1381 actcgcggaa agcgtcaaat gatttgagca atgccatatc ctgggttctg cgtgtcgcaa
     1441 cggtattcag atattgttcc gcggcatcac tcagagctgg cgtatgttcc ctgcctactg
     1501 attcacacag gtaatcaacg aagccaaaca ccatttctcg ttcgcgaggt gtgatcgccc
     1561 caccgatcgg agtccagtaa tcgaatccga gttgcagaag tttaaaaaaa cgcttatgga
     1621 atgcgtagtt acgaacgcgc ttaaaatcag cgtgtatcca ctcgcctatt ttgatttgat
     1681 acagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa accagaagaa gtttgtttga
     1741 ccagttgtat atgcgccatt gctatctcca atggcgctgt aggttgccag ttgttcaggc
     1801 tggcttacga attataactc attcccgaac caccttgaaa ccgagccttt ctaggtattc
     1861 aatgaacgcc tcgatagata aaatcacatg atcatcagga attaacgttg tgtagataac
     1921 gtctccattc tcaacgcgca cagcatagag gccattttca ctaaaaattt cacgcaattc
     1981 ttcgattttc atcttcagaa tccttccaga taaatagctc ttcccttctc agggtccatc
     2041 cctcttatcc ctgcgcgcta cttaagtgca tcgattctag tcaggcatac ccgcttatca
     2101 acaaacgaag gtcggtttaa tggaggagtc ggctaaaact tgatctgtaa aagtaaaaaa
     2161 cccgccgaag cgggtttagt gcgggtgcgt taaggatgcc tgacacatga gaggtggcga
     2221 gggatttctc cctcgcctgg tctcttactc ctcaggttcg taagctgtga agacagcgac
     2281 ctccgtctgg ccggttcgga ttcgtacctc gcagaggtct ttcctcgtta ccagtgccgt
     2341 cactataacg gttaaacaga tgacgatcag ggcgattaac atcgcctttt gctgcttcat
     2401 agcctgcttc tccttgacct ttcggtccgt aagaggctaa tctctatgtg tcgcatagat
     2461 atggcctcag attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct
     2521 ctgtctgcct tacggcggca tgcccgaggc agacagcctc aagcacccgc agcaattcta
     2581 cttaactctc gctttacagc aaaccgtttt tgcccgatat gggaattccc atacggaatg
     2641 aattcagttc cccaggcgat ccatcaaaaa cacaaccagg cagtaaacac ccacaacagc
     2701 aataacagcc agagcgcctt ccattaccag tgaaatatca tccgacatat tccctccctt
     2761 ggtgtgaatt ccggcgaacg tttttacccc caccgacaaa taacatatac tagaaaagca
     2821 atagctatag caacgcctgc aaatgcatcg ggccggctca ttggtgcgcc ccctgtatcg
     2881 cttctactgc gatctgactg gcgtatttgt taatggtaac gataagttct tgctcggcct
     2941 catccagaca accaccgata cctcgcctgt caccttcaga agcatcgaaa tctgcacgaa
     3001 ccctggcgac cttcaggatt gcggacaaca cctcatcagg gattgccgga gagttggttg
     3061 acgtttccga ggttatccga aaattattgg ttgacgaatt cttattttcc cgaaagtttt
     3121 cggcatgaag catggcggtg ttatacccat ccgcgaaaat ctctgcttct tcgttattca
     3181 gttcggcacc aagtttaagc gcaatagctt ttgccattga ggcggtaatt tgatggcatg
     3241 gctgacgtcc atgaagcatg gcgtcgctcc gctctatgcc atccagcgca attcgaagcg
     3301 catgaattgt agtaatgcta tcgtttgggg ttattccgta tcgttcgaat acagcgatat
     3361 ggttgcgcat aatcgcaggt gtaagctctt tgtaagcaag agcaagaggt ccagatacat
     3421 cgttcagcac taccgtcatc ggtgaggcgg cgtagacttc aataatccca gtatcaatag
     3481 gccattcccc atccttgata tagtaacttg ttccgtcaac ttgctgttct acaatgtgga
     3541 aagcacctat tggttctgct tccagcgatg ccagtgcaat tttgaataac tcgccctcta
     3601 cccgtgccat ccctgaattg gggtagcatt tcgcaatcgc tatttttaat ttggcttctt
     3661 cgattaattt ctctttggtt aattcagtca cttttcatta ccgccctttc gggcggcctc
     3721 ctgacattaa tcgttgtgat aactcatagc ttcatttgca gcatcaactg gatcaacatc
     3781 ccaccagcaa taatttgggt cgacaccttc aggtgtccac ggctctaatt cattttttgc
     3841 cgcattctcg tcgccagtaa ttttaaaaat ctgctctgag aactttttca cccactcgtt
     3901 atatttttca gcgttaataa ctttctgtgc gtttgacatt gatatacctc cggttaagga
     3961 ttaaattttt aacagagcta aatttaatta ttcagttctg gattttgtca ccctgcgtat
     4021 ccgcgctttc acgttacgct caatctgaat tagcttttct atatttctcc gcctttcccg
     4081 ttcctcctgg cgcaagatcc ttacatcatc tgccagtctg gtttctcttt tcgccacaga
     4141 gagcatccag tcaaatggct ccacaactgc accgcagatt ttacagcgga cctgacgctc
     4201 tttttcgtca acccggacag aggcgtgatg gcaatatggt ctttccgatg gctcatagag
     4261 aaaattaacc tgattacgtg ggtcatcctc ttttaccgga aataaaacga tattgcttaa
     4321 ctcatcttct ggttttattt ccatgctcct ctcctttgat gcgaatgcca gcggtaattg
     4381 aagcctgata gctaatttca ctcacagtac cgcctcctga aaattgccct gatagaacgc
     4441 cagtacacgc tgcataactt cgctctggcg gcactcacga caaattatgt tctgccgtct
     4501 gttgtaacgg cgtatttctc cgtcaggtaa ctttcgaatc agtgtcgggt cagcagcctt
     4561 ctccggtgtc ttacgccata cgcgatacgc ctgctctgat ggaaataccc cgcaaccaga
     4621 gagccagaca tcaccactgg ccgcaagcgc accagataaa cgacgaatag cggtcttact
     4681 gacacccgtt ttatctgcct gttgtcgaaa agtttctcgt ccgctcgggc gcacgaattc
     4741 cacaatgcgc gccttcactt cttcccgctc ttct
//
LOCUS       DAZFDC010000218         4733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFDC010000218 DAZFDC010000000
VERSION     DAZFDC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.219"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(90..941)
                     /locus_tag="ACHJ48_004637"
     CDS             complement(90..941)
                     /locus_tag="ACHJ48_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO4710028.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGTEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGIWQLHNAERCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QTRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVGER"
     gene            complement(1015..1257)
                     /locus_tag="ACHJ48_004638"
                     /pseudo
     CDS             complement(1015..1257)
                     /locus_tag="ACHJ48_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            complement(1482..2585)
                     /gene="zorE"
                     /locus_tag="ACHJ48_004639"
     CDS             complement(1482..2585)
                     /gene="zorE"
                     /locus_tag="ACHJ48_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HFO4710029.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLPNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPAKL"
     gene            complement(2589..3296)
                     /gene="zorB2"
                     /locus_tag="ACHJ48_004640"
     CDS             complement(2589..3296)
                     /gene="zorB2"
                     /locus_tag="ACHJ48_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
                     /protein_id="HFO4710030.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            complement(3303..>4733)
                     /gene="zorA2"
                     /locus_tag="ACHJ48_004641"
     CDS             complement(3303..>4733)
                     /gene="zorA2"
                     /locus_tag="ACHJ48_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HFO4710031.1"
                     /translation="ESIDTSIPVLLGGLKTAFITSIVGMFFAILFNGMDAFFFANKRS
                     ALAENNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLIAQIKLLRTEISDSSQA
                     QLANHTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDFNQNLTEQFGENFKALD
                     ASVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIWEECKEIPLAMSELREV
                     LQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGELLKSGAANVSASLEQTS
                     QQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNSSETLTSTLGETITEMK
                     QSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQLSSNADNMFDSIQTSF
                     DKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQSLGNALLSISKGFVGN
                     YEKLIKDYQIVMGQLQALISANKHRG"
BASE COUNT         1282 a         1003 c          910 g         1538 t
ORIGIN      
        1 atttataggt ggctggtatt taatattcct gcatcatatc tctgatgcag gagcagagaa
       61 tggctataaa ttattagtaa acagaatgtc tatctctcac ccacaaaccc ctcataaggg
      121 taatttttac gcacctgcgg caacgcaata gttttcccgt caaaatccca gaataatctc
      181 tcaacgatcc ctccgccgtt taccgcatcc gagaccagta cccgcatatt ttcatccagg
      241 ccaatcgatc ctttatcaaa ggctttatgg tgtattgcgc aaagcgccag accattgggg
      301 atttcacaag ggccgccgtg ctgtttccat tttatatgcg ccgcttccag cgcgacggtg
      361 gtgtcatcgt ggcgcatatt aaagccacat attgcgcact ggtagttata agcgcgtaat
      421 acatttttgc gaaataacgg gtcgcgctgt ttacgggttt gctggagatc aaaacccagc
      481 tcgtcagcga tttcttcctg aatactctcg gtgaagtgcg cttcgagtat ctgttgcgcc
      541 agagtattga tgagtttttt attgccggtt accagggcgt agtgctgttc atcgaaaccg
      601 cctgcaacgt ggtattcgtt cagttccttc actggcggtt gtcggctgct ccctgctgtt
      661 gagcaacgct cggcattgtg taattgccag attccgtcgc cttgtaatcg ccagaacggc
      721 atatcaggtc ggtactgtga acgctgtggc ccaaaacgtt ccagtaaact gtgcagaggt
      781 tcgtagattt ccgtgccata atcgaaaagg cgcggatgtc cattcaggta tcccgctaat
      841 acgtatagca gtaacaatgg tttatgcggc gcgcgctgtt cacccttgtg ccagattttt
      901 atgttggcaa ttgcctgctg tagcgattta ctggaagcca ttgatatccc aggcagtgaa
      961 ataattgcaa tcatgctcgc aaattttctg ctgttcaagc ctgcaagtga agatccattg
     1021 ctgtgttgag ggatcaaaag acaacaaaaa cttattgaag aaataatctt ctgctttttt
     1081 gtcgaaataa tgggcattga tcttgtctat agctgctgaa tcttttccag cctcgttgag
     1141 atcttcaaaa tagccattga agatgttctg gttaacaccg ccagtaacat aagccttgcc
     1201 gttgtgtaca aaagtcacat tacccgccat gcccatcggc gcgtgcgaca tcaatttatt
     1261 gctttattca ttgtgactgt cttctgtcta ccaagcttta accttcaccc atatgaggga
     1321 agttttaagt atgaggcgaa ccatagcata tgcttttata cctgttacac cataaaaatc
     1381 agacaagtta tgccataaac tacttttgta atttgagcca caaatcaggg aaattttatt
     1441 tatcttccgt gaaagtctta actttcacgg agttttagaa tttacaattt tgctggcgta
     1501 aaggctcctg tttgtatttc tttaaccagc tcacccacgg tatatattct ccatccttcg
     1561 acttcggcgg gagtaaggtt aactgcaatt ttaaggcctg gccatgcagc ttctacggtt
     1621 gcaataattt catgttctga gtttggaaga tctaacccag gaacaggagg ttgaaagcct
     1681 ttatgttgca tcatattaat atcaccatgc actgatggat cagtcaggtc atatatttca
     1741 ttccaggatt ctgcttttaa taacccttgt tgggaacgta aacgttgaat gatagccatc
     1801 tctgcttggg taacgaatat accttcgtgg aggggctgtt ttcgatggca atccttgcac
     1861 agaacaagaa ggttttcatg gtgattatca tatttgatgc catttttatg atggacatgg
     1921 cacaagtttt tggcggttga taaattcacg ccacaatcat tacaaacata atttgccttt
     1981 tctcgtattt ctttagatat ttccttccag ttttcgacat acccactatt tgttttgtca
     2041 tagataccct taggcatatg acgaaaacaa gtactataat ctgaaaaaaa ctcatttaat
     2101 gagaatgatt taaaaatctc tctttttcgt tgtttatcaa tacttccttt ataattaagt
     2161 cgttcgaggc aattcataca tacccgtaaa tcaacatcct ggctccgacc tgaaccatca
     2221 tcaatttcga ataaaccatc tatgcggtta gttgcatgat acctttcaaa tcgattttta
     2281 tggcgcattt catcaagagt tctgcaccag gcaatatgaa aacgttttcc tttttcgcca
     2341 tctacgatag ccgcatcata acgccctgaa tggtcccgaa tatataaaag aacctggcgg
     2401 ccatgataag agataagccc gctaccaggg tcaagatctt cgatactaac ttctttaccc
     2461 gtggataact caatatctat aggatcaaag tcaatagggg attcatccag aaaaaaatct
     2521 actccttctg gtaacatttt cttccctaat tgaataagtt ctgaaatgtc gatagataat
     2581 ttcatctctt actcctgaat aatctttcta atctgcaact cggcatttgt tacaactttg
     2641 aaggtgacac gacgagagcg attaggatcc tccttgccgg ttttatcaag aatgggatgt
     2701 gcagatgaat aacctactgc ggcaaattta cttttaaccc attgttggtg tgtcgcgata
     2761 ttttttatgt cataaacgta ttgtaatact gcacgtgtac gaccttgcga tagtgccata
     2821 ttattaaaat aagcaatatc aggattcgtt gttcctgtcc agtcagtact tgtgtgacct
     2881 tcaatgcgga cttcagtaat atgttcctga taattatcta gaacttttag gtagcgagga
     2941 aagaagtcgt caagaatgag tttaaacttt ggttttagct ctgtgcttcc taagccaaat
     3001 aaaacatccg gtgatttaaa tcgaacctcc agagtctgtt tatcgatctc tgcatcccag
     3061 tcttgtaaat cctttgcaaa ctcaatatcc agagcattat aaagctgtaa ctgagcattc
     3121 tcgtaggcta cggcaacttc tttaattttt tctttttcaa tacgtacgta gtgcatataa
     3181 gcaatagata tgaacataaa aaccatcatc agcccagcca ttaggtccga catggatacc
     3241 caatgttcat tatcttgatc ttttttaggt aattgtttcc ctataatctt atccatgatc
     3301 gattacccgc gatgtttatt agcagaaatt aatgcttgta actgccccat aactatttgg
     3361 taatctttaa taagtttttc atagttaccg acaaaacctt tgctgattga aagcagagca
     3421 ttacctaatg attgcatttc acggttcatt tcacgttcag ttgcttgctc aaaagcatgt
     3481 aactgctcgt tgatggatag agcaaatttt tcaattgatt ctctgacttg agaagtcagc
     3541 cctgcacttg ccttatcaaa tgatgtctgg attgaatcaa acatattatc ggcattactg
     3601 gatagttgat ggttaatctt ttcaccagtc ttactgaaca tatcaatcac attcgtcgta
     3661 ttttgttcca tatttctatg caattctttc gagtgcgact ctagtgattt caggaattct
     3721 tcaccacttt gtttcatttc agtaattgtt tcacctaacg tgctggttag ggtttcggag
     3781 gaattgctga catcatgtgc catcgaggca aatgcttgtt gtgtttgggt aaccgattgt
     3841 ctgaatcctt cggtaccttc atccagggca acgcgcattg aatctgcatt aagaagtatt
     3901 tgctggctgg tttgctcaag agatgcacta acatttgcag ctccggattt cagcagttca
     3961 cccacttcag ccattttgtt ctgtatttca ggtaatacgg ttgtagcttt atcgcggatg
     4021 gcgacaaagg tttctaaatg gcgggagagt tcgctgattt gatgttggtt cacctgaagc
     4081 acttcacgca gttcagacat agccagagga atttctttac attcttccca aatccctgca
     4141 accgcagttt ttgtttcaac cagggactcg acactttgtt gatattgttc tgacatctgc
     4201 tcaatttgcg ttttataatt tccctgccac tcaacaagtt tttttacaga ggcatcaaga
     4261 gctttaaagt tttcaccaaa ctgttcagtt aaattttgat taaaatcaat aatgacttgt
     4321 cgcaaagcat caataatttg ttctgtagca ccttttgcca ttagatctgc aaattgttca
     4381 agttgttccc aaagcttatt actgaaatga gtgtgattag ctaattgtgc ctgcgaggaa
     4441 tcgctaatct cagtacgtag taattttatt tgagcaataa gagaaccttc actatcaccg
     4501 ttaatacccg agactaattt agtcagagtc tggttctgct cttttaattc atgatagata
     4561 tgttcaggtg taacagattc agggttattt tctgctaacg cacttcgttt attggcaaaa
     4621 aagaaagcat ccattccatt aaataaaatg gcaaaaaaca tacctacaat gcttgtaatg
     4681 aatgctgttt ttaggccacc taataatacg gggatgctgg tatcaatact ttc
//
LOCUS       DAZFDC010000219         4642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFDC010000219 DAZFDC010000000
VERSION     DAZFDC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.220"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            211..1368
                     /gene="acrE"
                     /locus_tag="ACHJ48_004642"
     CDS             211..1368
                     /gene="acrE"
                     /locus_tag="ACHJ48_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO4710032.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1380..4529
                     /gene="acrF"
                     /locus_tag="ACHJ48_004643"
                     /pseudo
     CDS             1380..4529
                     /gene="acrF"
                     /locus_tag="ACHJ48_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
BASE COUNT         1128 a         1131 c         1206 g         1177 t
ORIGIN      
        1 aattctgcct ctttcaaccc gcgtcaaaat aaaacagtag aatattaatc tttttttgtg
       61 tttatgtgcc ttgagacgcc tgtattcata actattcctt acatcgacga atgataattt
      121 gtaggatagc gaactgtatt tttctttctg cgagttaacg cgttgccttt ttgggtaaat
      181 aacgcgcttt tggttttttg aggaatagta atgacgaaac atgccaggtt tttcctcctg
      241 ccctccttta ttctgatctc cgcggcttta atcgccggtt gtaacgataa gggagaagag
      301 aaagctcacg tcggtgaacc gcaggttacc gttcatattg taaaaacggc cccgttagaa
      361 gttaagactg aattaccagg ccgcaccaat gcttatcgta tagccgaagt tcgcccacag
      421 gttagcggga tcgtactgaa tcgcaatttc actgaaggca gcgatgtgca agcaggccag
      481 tccctgtacc agatcgatcc cgcgacctat caggcaaatt atgacagcgc gaaaggcgaa
      541 ctggcgaaaa gtgaagccgc cgccgccatc gcgcatttga cggtaaaacg ttacgttccg
      601 ctcgtgggta cgaaatacat cagccagcag gagtacgacc aggccattgc tgatgctcgc
      661 caggccgatg ccgccgtgat tgccgcaaaa gccacagtcg aaagcgctcg catcaatctt
      721 gcttatacca aagtgactgc gccaattagc ggacgtatcg gcaaatcgac tgtgaccgaa
      781 ggcgctcttg tcactaatgg gcaaacgact gaactggcaa ctgtccagca gctcgatcct
      841 atctatgttg atgtgaccca atccagcaac gattttatga ggctgaagca gtccgtagag
      901 caaggaaatt tgcataagga aaacgccacc agcaacgtag agttggtcat ggaaaacggt
      961 caaacctatc ccctgaaagg tacgctgcaa ttctccgatg tgaccgttga tgaaagcacc
     1021 ggctccataa ccctacgtgc tgtcttccct aacccgcaac atacgctttt gccgggtatg
     1081 tttgtgcgtg cacggattga tgaaggcgtc caacctgacg ccattctcat cccgcaacaa
     1141 ggcgttagcc gcacaccgcg tggtgatgca accgtgctga ttgttaacga taaaagtcag
     1201 gtggaagtgc gccctgtcgt tgccagtcag gcgattggcg ataaatggtt gattagcgaa
     1261 ggtctgaaat ctggcgatca agtcattgtc agcggcctgc aaaaagcgcg tccgggagag
     1321 caggttaaag ccactaccga tacccccgca gatactgcat cgaagtaagg taatctgaca
     1381 tggcaaactt ttttattcga cgaccgatat ttgcatgggt gctggccatt attctgatga
     1441 tggcgggcgc actggcgatc ctacaattgc ccgtcgctca gtatccaacc attgcaccgc
     1501 ctgcggtttc tgtttcagca aactatccgg gcgctgatgc gcagaccgtg caggatacgg
     1561 tgacgcaggt tatcgaacag aatatgaacg gtatcgataa cctgatgtat atgtcctcca
     1621 ccagcgattc cgccggtagc gtgacaatta cccttacctt ccagtccggg accgatcctg
     1681 atatcgcgca agtgcaggtg cagaacaaac tccagctcgc cacgccgttg ctgccgcagg
     1741 aggttcagca gcaggggatc agtgttgaaa agtccagtag cagctatttg atggtggcag
     1801 gctttgtctc tgataaccca gacaccacac aggacgatat ctcggactat gtggcctcta
     1861 acgttaaaga tacgcttagc cgtctgaata gcgtcggtga cgtacagctt ttcggcgcac
     1921 agtatgcgat gcgtatctgg ctggacgccg atctgctaaa caaatataaa ctgacaccgg
     1981 ttgatgtgat taaccagttg aaggtacaga acgctcagat cgctgccgga cagttgggcg
     2041 gaacgccagc gttaccaggg caacagttga atgcctcgat tattgctcag acgcggttaa
     2101 aaaatccgga agaattcggc aaagtgaccc tgcgcgtaaa cagtgacggc tcggtggtgc
     2161 gtctgaaaga tgtcgcacgg gttgaacttg gcggtgaaaa ctataacgtt atcgctcgca
     2221 tcaacggaaa accggcggcg ggcctgggta ttaagctggc aaccggcgcg aatgctctcg
     2281 ataccgcgaa agccattaag gcaaaactgg cggaattaca gccattcttc ccgcagggaa
     2341 tgaaggttct ctacccttat gacaccacgc cattcgtcca gctttctatt cacgaagtgg
     2401 taaaaacact gttcgaagct attatgctgg tgttcctggt gatgtatctg ttcttgcaga
     2461 atatgcgagc aacgctgatc cccaccattg cggtacccgt ggtgttgtta gggacgtttg
     2521 ccatcctcgc cgcttttggt tactccatca acacactaac gatgttcggg atggtgcttg
     2581 ccatcgggct gctcgtcgat gatgcgatag tggtggtgga gaacgtcgag cgcgtgatga
     2641 tggaggataa gctcccgcca aaagaagcga cggaaaaatc aatgtcgcaa atacagggcg
     2701 cactggtggg tatcgcgatg gtgctgtcag cggtatttat tccgatggca ttcttcggcg
     2761 gttctactgg ggcaatttat cgtcagttct ctatcaccat cgtttcggca atggcgcttt
     2821 ctgttctggt ggcattgatt cttacccctg cgttatgtgc aacactgctc aaacccacct
     2881 cagctgagca tcacgaaaat aagggtggtt tcttcggctg gtttaatacc accttcgatc
     2941 atagcgttaa ccactacacc aacagcgtcg gcaaaatcct cggttccaca ggacgatatt
     3001 tactgatcta tgcgctgatt gttgcaggaa tggtggtgtt gtttttacgt cttccgtctt
     3061 ccttcttacc tgaagaggat cagggtgtct ttctgaccat gattcagtta cccgctggcg
     3121 cgacgcaaga gcggacgcaa aaagtgttgg atcaagtgac ggattactat ctgaagaacg
     3181 agaaagcaaa cgttgaaagt gtctttacgg ttaacggctt tagcttcagc ggccaggcac
     3241 ctccgcaacc ccatgttttc acataactgt tgcgttgacc aattgaatct acaggtatgg
     3301 ccttcgtcag tctgaaaccg tgggaagagc gtaatggtga tgaaaacagt gcggaagcgg
     3361 taatccatcg tgccaaaatg gagttgggca agatccgcga cggttttgtc attccattca
     3421 atatgccagc cattgttgaa ctgggcacgg caacgggttt cgactttgag ttaattgata
     3481 aggctgggct gggtcacgat gccctaaccc aggcccgtaa ccagttgctt ggtatggcgg
     3541 cgcaacatcc tgccagctta gtcagcgtgc gtcccaatgg cctggaagac accgcgcagt
     3601 ttaaactgga agttgaccag gaaaaggcgc aggcattagg tgtttcactt tctgacatca
     3661 atcagaccat ttcaacggcg ttgggtggga cttacgttaa cgacttcatc gaccgtggtc
     3721 gcgtgaaaaa ggtgtatgtt caggcggatg ccaaattccg tatgctgcca gaagatgtcg
     3781 ataaacttta tgtccgcagc accaacggcg aaatggtgcc attctctgcc tttaccactt
     3841 cacattgggt gtatggctct ccgcgactgg aacgctacaa cggtctgccg tcaatggaga
     3901 ttcaggggga agccgcgcca ggaaccagtt ccggcgatgc catggcgttg atggaaaacc
     3961 tagcgtcaaa attacctgcg ggcattggtt atgactggac gggtatgtcg tatcaggaac
     4021 gcttatcggg aaaccaggct cccgctctgg tagcaatttc ctttgtggtt gttttcctgt
     4081 gccttgctgc actctatgaa agctggtcaa ttcctgtctc ggttatgttg gtcgtgccgt
     4141 tagggattgt cggcgtgctg ctggcggcga cactctttaa tcaaaaaaat gacgtctact
     4201 ttatggtagg cttgctaacg acaattggct tgtcggccaa aaacgctatt ttgattgttg
     4261 agttcgctaa agatctcatg gagaaagagg gtaaaggtgt tgttgaagcg acactaatgg
     4321 cagtacgtat gcgtctgcgt cctatcctga tgacctctct cgcctttatt ctcggcgtat
     4381 taccgctggc tatcagtaac ggtgccggca gtggcgcgca gaacgcagtg ggtatcgggg
     4441 taatgggagg aatggtctct gcaacgttgc tggcaatctt cttcgtaccg gtgttctttg
     4501 tggtgatccg ccgttgcttt aaaggataaa tctgaaacat aaaggcgctt tcgggtgcct
     4561 ttatgttttc cagtgaaacc cataaaaatt aataagatat tcttttgctc actctttaaa
     4621 agctttctat agctcccgct cc
//
LOCUS       DAZFDC010000220         4532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFDC010000220 DAZFDC010000000
VERSION     DAZFDC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.221"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(55..375)
                     /gene="chbB"
                     /locus_tag="ACHJ48_004644"
     CDS             complement(55..375)
                     /gene="chbB"
                     /locus_tag="ACHJ48_004644"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO4710033.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(675..1013)
                     /gene="osmE"
                     /locus_tag="ACHJ48_004645"
     CDS             complement(675..1013)
                     /gene="osmE"
                     /locus_tag="ACHJ48_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO4710034.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1215..2042
                     /gene="nadE"
                     /locus_tag="ACHJ48_004646"
     CDS             1215..2042
                     /gene="nadE"
                     /locus_tag="ACHJ48_004646"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO4710035.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREACIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2272..3159
                     /gene="cho"
                     /locus_tag="ACHJ48_004647"
     CDS             2272..3159
                     /gene="cho"
                     /locus_tag="ACHJ48_004647"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO4710036.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3119..3694)
                     /gene="ves"
                     /locus_tag="ACHJ48_004648"
     CDS             complement(3119..3694)
                     /gene="ves"
                     /locus_tag="ACHJ48_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO4710037.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3897..4382)
                     /gene="spy"
                     /locus_tag="ACHJ48_004649"
     CDS             complement(3897..4382)
                     /gene="spy"
                     /locus_tag="ACHJ48_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO4710038.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1134 a         1100 c         1083 g         1215 t
ORIGIN      
        1 atattgcggt attgatttat gaaataactc tttgacggga aaatttaaaa taaattaatt
       61 tgctgcggct tttttaatcg ctgcaaccgc agccttaagc acgcctaaac catcgacttt
      121 gccataaagc agcgagtcaa ttacttcaac cggtttgttg ggtaacaaac gctggatttc
      181 gggcaacata taagcaatct gcggccctaa taacacgaca tcggcattct gacctttttc
      241 accagccagt gtttccggaa atgcttcaat aatgaccgga acttcatatt tttctgcctg
      301 tgcgcgcatt tttgatacca gtaaagaggt agacatgccc gcagaacaaa acagataaat
      361 gtgtttcttt tccataaaac tgccctcatc gacgattatc tgtcagccag acactccgca
      421 agccttaacc tgcttccatg ctctgggtaa cttgcgaaac caacatgact ttttttgtgg
      481 ctgaaaggag tatacggtat agacctgagc aatagtattt ctttgacctt ataaattgtc
      541 tctccttgac ctgcggttat gaccctcttc acatttcggg caaataattc gcgaagataa
      601 ttaagaactc aggccaaaaa aaaaccggcg caatggccgg tttccgttgt tactcaagct
      661 ttcagacgaa gtgattactt cgcagcctgt ggatcagtgt cgtattcagc acaggtctga
      721 taaccggagt tgatgacatg tccggtatca tctaacgcga caaagtaggt ttctgcttta
      781 ccatcacgtt gacccaggat gtaggtctgg caagtaccac gagcatggat catgctcact
      841 tcagacgaag gtttacccgc aatttgtgca acctgcgccc ggctcatgcc ttttttgacg
      901 tctttcacca caggctgtac aaactggtct ttggtacgat cataagccgt acaacccgcc
      961 agcatggtta ataccgccgc tgcactcaga attcctgcca tattcttgtt catattccgt
     1021 cctattgttt atcagcgtgt tagataagcc tggaatacat tgggcgcttt ttcaagccca
     1081 tgaacgaaac ggctccgctt tcagaggatt cctgtatgac gttttaacca ccattcagcc
     1141 cgctgtcgct tgtcgtttca gtagcaacgg gttagcttta aggaagtttt gtcttttctg
     1201 tctggagggg ttcaatgaca ttgcaacaac aaataataaa ggcgctgggc gcaaaaccgc
     1261 agattaatgc tgaagaggaa attcgtcgta gtgtcgattt tctgaaaagc tacctgcaaa
     1321 cttatccgtt cattaaatca ctggtgctcg ggatcagcgg cggtcaggac tccacgcttg
     1381 ccggtaagct gtgccagatg gcgattaatg agctgcgcca ggaaaccggc aacgaatcac
     1441 tgcaatttat tgccgtacgc ctgccctatg gtgttcaggc cgacgaacaa gattgccagg
     1501 atgccattgc ctttattcaa ccggatcgcg tattaaccgt taatatcaag ggcgcggtat
     1561 tggctagcga gcaggcattg cgggaagcat gcattgaact gagcgatttt gtccgtggca
     1621 atgaaaaagc gcgtgagcgg atgaaagcac aatatagcat tgcgggtatg accagcggtg
     1681 tcgtggtggg caccgatcat gcagcagaag ccattaccgg attcttcact aaatatggtg
     1741 acggcggtac ggacattaat ccactgtatc gtctcaacaa acgtcagggt aaacagttac
     1801 tggcagcatt aggttgcccg gaacaccttt ataagaaagc gccaacggcc gatctggaag
     1861 atgatcgccc ttctctgcca gatgaagtgg cactcggcgt gacctatgac aatatcgacg
     1921 actatctgga agggaaaaac gtacctcaac aggtcgccag aacaatagag aactggtatc
     1981 tgaaaaccga acataaacgc cgtccgccaa ttaccgtttt cgatgatttc tggaaaaagt
     2041 aataatttgc acctattgga ttgtgcaaaa aagagtaatt tgttcacgcc ggatgcggcg
     2101 tgaacgcctt attcgaccta caaaactatg caaattcaat acattgcagg agtcgaatag
     2161 gcctgacagg cgtagcacgt cagacggtgt aacctttgtc atcgacccgc ctctttttta
     2221 atcgcttccc ggctgttaca ctggatagat aaccagcatt cggagtcaac agtggtacgg
     2281 cgtttaactt ctccgcggct cgaatttgaa gctgcggcaa tttatgaata tcccgaacat
     2341 ttacgttcat tccttaatga cttacccacc cgaccagggg tgtatctgtt tcatggcgaa
     2401 agtgacacca tgccgctcta tatcggcaaa agcgttaata tccgcagtcg ggtgctttct
     2461 catttacgca ccccggatga agccgccatg ctacggcaat cccggcgaat tagctggatc
     2521 tgcaccgcag gcgaaatcgg cgctctgctc cttgaagcgc gattaatcaa agaacaacag
     2581 ccgctgttta ataaacggtt gcgccgcaat cgccagctct gtgccctgca attaaatgaa
     2641 aagcgcgtcg atgtggtgta tgccaaagag gtggattttt cacgagcccc caacctgttt
     2701 ggcctgtttg ccaataggcg cgcagctttg caagcattgc agagcatcgc tgatgaacaa
     2761 aaactttgtt atggtctgtt gggactggaa ccgttaagtc gcggtcgtgc gtgttttcgt
     2821 tcagcgctaa aacgttgcgc cggagcatgc tgcggtaaag agagccatga ggaacatgcg
     2881 ctacgcttgc gccagtctct ggagcgtttg cgggtggtgt gttggccttg gcaaggggcg
     2941 gtggcgctga aagaacagca cccggaaatg actcaatatc atattattca aaactggctg
     3001 tggctggggg cggttaattc gctggaagaa gcgacaacgt taattcggac acccgccggg
     3061 tttgatcacg acggttataa aattctttgt aagccgctgc tttccggtaa ctatgaaatt
     3121 actgaacttg atccggcgaa tgaccagcga gccagttgat ttcactgaac aacagtttac
     3181 cttgcggttg taaaagccgc agggtgtgcc tgccatcgaa ccagcacgcg ccctggtcgg
     3241 tagtgagcag tttgtcgccc agttgccacg ccccgttaat cacaaacacc accccgccgc
     3301 gtgagccaaa ggtagtaaac gtacgttcgg caattctgac tttcgcctta cagacatcca
     3361 ggcgcgtcat aatattgaaa tccatcgaca tctgccctgc cgtcagtttc gcttttacca
     3421 cctggtccgc cgcaaaggca aaaggctgta acggttttaa ggtatggtta aagtggtctg
     3481 cgctttcaag gaacatctcg ccgccttcca gcaacgtcac tatcctttcc ataccaggaa
     3541 acaaagaaaa ctccccattc gccgcgatgg atgcaatgct ggcacgccag taaaaatcac
     3601 gttttgccgg tgggaaagtg caaatttcgc gcgtttcacc ggcagcgttt cgccacaaat
     3661 tcaccgacat tttacgcata tcaaagtatt ccatactcgc tcccggcccg ttcttttcat
     3721 tgttatcgtt caggtaaccg tggatggtta tcggcaactc gggtgattta cgttagtggt
     3781 gatcaggctt tttttacaac tgtttgagaa gagaaaagaa aaccgccgat cctgtccacc
     3841 gcattactgc aaggtagtgg acaagaccgg cggtcttaag ttttttggct gaaagattat
     3901 tcagcagttg caggcatttt accttttgcc gctggacgtt ctgtcagacg cttctcaaaa
     3961 ttagcattaa attgcttttt ctgttccggc gtcaggatgt tgtaaatttt gttctgggtt
     4021 tccatgtgcg ccagcatgtt agctttgcgc tgttcttcca tttttgcgat ctgcgcttca
     4081 gcttttgctt tatcgaaggt atcgctggca atgatgtcat gcattgcgcg acgttcttcc
     4141 agcggcggac gtttcatctg gtcacgctgg cctttcatga tttcgcgaat ctgctgtttc
     4201 tgcgcgtcgg tcaggttcag gtctttgaac atcatgtcct gatgcggacc gaacttgcct
     4261 ttgtggtgca tcatcggctt cgcgtcagcc ggtgctgcgg tagtggtgtc tgcggcatgg
     4321 gccaggttag ccgcgccaag agccagggta gaggcaacaa acagtgcagt taatttacgc
     4381 atattctata tccttccttt cagttattta ttacggcttt cttaagtagc gtgccgtgtt
     4441 gacgagatta actttactga ctttagcgtc aattaatcag agcaacggta aaacaatgaa
     4501 agtgtaaaaa acacttttgc gccaattata ga
//
LOCUS       DAZFDC010000221         4436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFDC010000221 DAZFDC010000000
VERSION     DAZFDC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.223"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(248..2416)
                     /locus_tag="ACHJ48_004650"
     CDS             complement(248..2416)
                     /locus_tag="ACHJ48_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO4710039.1"
                     /translation="MIFPFGLNESQLLAVERAFSSQISVIEGPPGTGKTQTILNIVAN
                     ILIQNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVL
                     PDSPSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFS
                     HKYRFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSL
                     QLSYYEKNIREKQISLNEYEEAFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFS
                     AETYRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCA
                     RNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHY
                     RCHPKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHY
                     WDEESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQH
                     SRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFE
                     SPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHS
                     QIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQA
                     ERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(2413..2928)
                     /locus_tag="ACHJ48_004651"
     CDS             complement(2413..2928)
                     /locus_tag="ACHJ48_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710040.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKNVIRHGV"
     gene            complement(3169..4215)
                     /locus_tag="ACHJ48_004652"
     CDS             complement(3169..4215)
                     /locus_tag="ACHJ48_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO4710041.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
BASE COUNT         1247 a         1080 c          880 g         1229 t
ORIGIN      
        1 cattttgcag gaagtattca ctgtaataga attaccccct gataaagttt attcactaag
       61 gaattattca acagaaacat tccctgttag ctttgagcaa tagcacttat ttttatatgt
      121 atcaggaata cgtacgttga tgtaaccaaa acaaagaagc ctgtgtccat ggggttcgta
      181 caattgtgtg tggcacatga ccaaatcagg tttgagacct cccgctgcaa tattttttat
      241 tcagatttta gcctgtcagg cctgacagaa acgctcccaa cttcccttct atatcactgt
      301 ctatagtacg taatcttagc aatggcaggc cgcatttctt cagtatactg tttttaagtt
      361 catcacgttc agcctgaaca ctggtcagat gataacctcc atccacttca ataaccccta
      421 atggagtctt cccgacctta tagtaaacaa caaagtcaca actggcccgg ttacgcatga
      481 ataactgttc acggtgtgta aaatcccccc gctccagcaa caccaactga ttcagcgcta
      541 tttgtgaatg aaacatcata ctccgataac tatcctgcga caggatatcc cgcaacaggc
      601 aggccacaat ctgttctgac ttaaagtgtg aatcatttga attcaatcta ctattcagtc
      661 gttccagtga tttgtcatac tcactgtaca gcaaatcaaa tgctgatatg accgggctct
      721 cgaaaatctc tccgtcatca gcgtagtatt tgatgtaacg aatcagcgcc gcaatatgtc
      781 cagcgtggcg ttcaaataca tcattaccag tcaccagcgt aaatttattt cgggcccttg
      841 aaacagcgac attcaccagt tcaggattat caacaaacgc gatgttccga ctgtgctggc
      901 tgctgcgctt cttatccagc acagtggaaa acacaatttc atcgcactcc cgtccctgaa
      961 atttatgcac cgtcgatttc acaaaatcag caggcagcac tttttcagcc agattaacct
     1021 gtgcattata tggggcaatg tatcccctgc tactttcttc atcccagtaa tgaccttcca
     1081 gagattcaag ctccctgaga tttgaaaaat tacgggtatg gtttcctttt gccgtgatga
     1141 ccagagataa tgaggcttca ccggaatcaa ccgttaacgg tatcagagca ttatcataaa
     1201 actgcttatt acaaaattga atgattttag gatgacaacg ataatgctct ttaagaaggg
     1261 ttactggcac catgttcctg aacaacatac aaaccgaatc aagtagacta tatttctcac
     1321 aattatagta ttctgccggt ggagatgggc tgttcgggag taatacagga acatgtggaa
     1381 gttgcttacg atcccctaca acgattacat tacgggcaca tcccagtccc agaatgcccg
     1441 gaacaatatc ctgctgcgag gcttcatcaa taataacata atcaagaagg gcccccttgc
     1501 cgatagagtt aataatcgaa tgcgtactgc taccaataat cggaaaacgc tttataaaac
     1561 ggtcaaattc atccctgtat gtctctgccg agaatgaact tcttgttgaa acatttcttc
     1621 gcagatgttg ctttaaataa agcatagacc atgatgtcag gcgccccagt aaaattttaa
     1681 aatcagattt tttaaatgct tcctcatatt cattcagaga aatttgtttt tcccggatat
     1741 ttttttcata ataagaaagc tgaagagaag taaatagcgc cagccttctc tccggaatca
     1801 tcaatggttt gactttcagt atcatgaagt ttaacaacaa atcaattctg tttctgaatc
     1861 caatccgcct gtcagaaagg taatgaatat aagccatcag gtcggttgtt ttttgtgagg
     1921 aaaatcggta tttgtgagaa aaaagctcct ccggtcgaag attctgactc tgccattgct
     1981 gcaggtattt atactcaata ttcagctcat taatttctgc ttttaacgaa gcgacctggt
     2041 taatggcatt caggtgtttt ttcacttgtt gaagtacatc atcaatcgca tttgcagacg
     2101 gtgagtcagg taagacttcc tcagaacgcg aaatcgacgt tgaaaaaaac tgttgcctgt
     2161 tctccgtact ccccagtcgt gccatcacat atcccagttg ctgtttgtcc atcttttcat
     2221 aaacattact gacagctgag ttattattgg acaaaattgc cactgtttta ttctgaatta
     2281 atatattagc aacaatattg agtatagtct gtgttttacc tgttccaggt ggtccttcaa
     2341 taactgaaat ctgtgaggaa aatgcccgtt cgacagccag caactgactt tcattaagac
     2401 caaacggaaa aatcaaaccc catggcgaat cacgttttta ctctgtcctg aacagtatgc
     2461 atgcaaggcg gtatccggat gaggaactat tttctttatc tgcctaagga tattctcatc
     2521 gatatttctg ttattaccac gggcataaaa aatacgttca ttagccaccc tacacaagta
     2581 attaaacacg gcatgctctg taaatgctga acactgttga aagtcaaggc cggtcgtttt
     2641 catgatgtag ggtcgttcac cctcgcggta ataaacagca gcatatttat caccatatat
     2701 aataaccagt tcagcccggg atgttacagc gttccctttc ctttcgagga aaacatttcg
     2761 caaatttaca gttggcgtta tctgacatac agaaagcgga cgagtatatt ttctcccgct
     2821 gcgaaaatga caggtcagca taacctctct gtcgtcattt ccagcccaca gcgtccagtc
     2881 ggtaatttta tgagtcttat tttcaccgtc aacacaaatc gtgaccatat tcttactcag
     2941 aaattatcat ttaacttacg gaaaattgtt aaggcactcc cgatattagc tccggaggat
     3001 tttccacaac tccgggactg ttaacatatt tctgcctgcc atatatacat tgaatttagt
     3061 cataacagtc aataccagaa ttacaggaat gcgatgccga tacgctggtt attgaatact
     3121 ggtagcccct gagcactaat tcatcacgat actctgtatt tactgttttc actgcttttt
     3181 acaaccattc ccccaggtaa aatgctgctc ctccggtgat ggctgtgtca gaaactgcca
     3241 tcccggaata gccacgctga ccgccagcaa tgccgcacag acaccagcca caaacggttt
     3301 cagaaacgtg ggcggggatt tctgaaggga gggtgactgc tccgcctgca ctttcccctt
     3361 gcgattatca aggctcagtg catccagacg caccgcgcca tttgccgcaa gcagcccttc
     3421 ccggttatcc acgcttcccg gcagactcag cgaaaaatcc cctgttcccg tctgaatcag
     3481 actgccttta cgatttgaca ggctttgtgc cgatatctgc agtgtgtcgg catttatcgt
     3541 accgccatta ctgctgaact gcccggcagt ccgggcggaa agcgtatcgg cactcagttg
     3601 cgcattctgt gtagacagat ttccactggt tgcattcagc tcagtatgtt gtccctgtat
     3661 ccggctgtta cctaaatcca gcccctgctc aatgtcccgt aaatccagca gagaaccttc
     3721 gtgtgccggg aatgaggaat acaactgaat atagtcatca ctgtccggtg tcagacgcac
     3781 atgtcgcaca acaccgggaa taataaccag ccggaggata ccgcttttta aatcctgcga
     3841 aggtgccaga acacgggtgg tgacataacc atgatcgacc agacggttct gcaatgtact
     3901 catcagcaga ttaattcctt tcgcccccag gcaatgcccg accgccccgt tggcgatttt
     3961 ttgtaaaggc agccagtgtg gtaacgcatc agcccccttc agttccgtct gtttaatctg
     4021 aaaacagggc gtttcaacag gaaaattaat cttatgggca aaagagccag gtgcagaaag
     4081 gcgaacatca ggggctgaag gcgtcagttg ttgctcaagg gcttcctgac gctgctgctg
     4141 aatgataaat gcttcatctc cgctacgccg gacatccgct gcctgaagag ataaaggcga
     4201 taataaagca gacatcagtg tcagtgacag caaggacgga cagcgccgga ataagctttg
     4261 ttcctcagtc ctctcgttcc tgcaacataa ttttccagtc aaacgaaaaa ccttttattc
     4321 atgtcatacc aacaatcacc atgaaccgcc ccgggaatcc tggagactaa actccctgag
     4381 aaagaggtaa acaggatgac taaaaataca cgtttttccc ccgaggtccg tcaacg
//
LOCUS       DAZFDC010000222         4430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFDC010000222 DAZFDC010000000
VERSION     DAZFDC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.224"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2384)
                     /locus_tag="ACHJ48_004653"
     CDS             complement(<1..2384)
                     /locus_tag="ACHJ48_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4710042.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLTRA
                     RVNQQNTENAAEKTGAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPINSIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSSILTDEITLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGER"
     gene            complement(2480..2668)
                     /locus_tag="ACHJ48_004654"
     CDS             complement(2480..2668)
                     /locus_tag="ACHJ48_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4710043.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            complement(2665..2853)
                     /gene="dicB"
                     /locus_tag="ACHJ48_004655"
     CDS             complement(2665..2853)
                     /gene="dicB"
                     /locus_tag="ACHJ48_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4710044.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            complement(3334..3486)
                     /locus_tag="ACHJ48_004656"
     CDS             complement(3334..3486)
                     /locus_tag="ACHJ48_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO4710045.1"
                     /translation="MDMLNLGNNESLVCGVFPNHDGTFTAMTYTRSKTFKTEAGAHRW
                     LARNAN"
     gene            complement(3761..4405)
                     /locus_tag="ACHJ48_004657"
     CDS             complement(3761..4405)
                     /locus_tag="ACHJ48_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO4710046.1"
                     /translation="MESLGFRLKRLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSTSGTKIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVEEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VPDPLKNEPSMTIKKLVIDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
BASE COUNT         1024 a         1079 c         1004 g         1323 t
ORIGIN      
        1 cgttcacctt cgaatggaat agtcgttctt gcgtcgaaat ccatcaccag tcccagagca
       61 accatcgttc ttacatcgcg atcattggtg taacgccacg ggcaggggat cccctgccgt
      121 tcatatgaac ggcgtaaaat cacgttgtcg aaagttgcac cgttacccca gacctgaaca
      181 aaaaattcac cggagttttc gtcgataaat tcccggaatt gcagcagtgc atcatccaac
      241 gtgatttcat cggtcagaat ggaggattgt gcttcgcgtg actgtttcag ccaccactta
      301 atggtgtccc gatcgatgac cccacctgcg gtttccagat cgatagtttt gctgaattct
      361 ggccccatct ctccggttgc cggatcaaaa aacttaccgg ctatagagtt tattggcgca
      421 tcaggatttt ttcccattgt ttcaaggtca atcatcagat ggtgccacaa cctgctggtg
      481 gatgagattt catgatgacc gttcacttta attaagggat ttgctgtttc gccagtttta
      541 ttatcgctgg cgtgatactg atcgctgcca gtgttctcct tgtgcagatg ttcagtgcct
      601 tccatttcct ccggatcatt tttctgagct tcagcctgat tctcttcatc gaatgtttcc
      661 aggtaggttg cgttccccat caccgcacca cagtcaggac agttgccgcc accggtctga
      721 ccgcatgcgg tgcaaacttt ttccagttcc tgttgcactg ctggttctgg ttgttgttct
      781 tctggcccgt tttgttgcgt atccgggctg ttttgttccg cttctggctc attttgtttc
      841 acgttgggct gattctggtc ctcagcgtcg cgactctgga tccctttcac ccatttcgga
      901 tcattcgggt cgctaatccc ttcaacaaat tcaccacgtg atgcagcaag caatttatcg
      961 gcgtcaggct ggctgatatt ggctgcctgc ataattttgt ttacttcgtc agcggtaact
     1021 tttaccggct ctgattgttc ggaatcttca gcggtatcca cattttgcgg taagcccgtg
     1081 tatgtgccat tttttcgggc aaaatattct tcttttgaga tttcggtacc gccggcagcc
     1141 agcgccttgt ccagaccaga aagtttgttt gccctgccgt atttttcccc gcccttatcg
     1201 gtaaagacga aataaaatgg tccttcacgc tctacagatg gttcagcttc caccaggatt
     1261 tcattttttt gagtatcgga ttctgccgtc tccactggag cagtttgtgc tgctgacggc
     1321 tggagagcat cagtagagct ctggtctgtt tcttcatgtt caaacacgcc ctttgtcgtc
     1381 aggtattcac tgatatattt gttcagtgca acgggatctt tgtgaatatc gatcggacgt
     1441 tcgcgaacaa ggccaaaaat agtctgacgg tcatagccaa gtgcttcggg ctgtttgcgc
     1501 attgatgctg agatacgctt ccagtcttcg cgatccttgt cgataacctc atttgtcgcc
     1561 cagcgatgga tggtaccgtc aatgtttcct gcatccacat caccgggcca gagggcgcag
     1621 gccagttcat tgtcgagtgt tttccatgtc tgtttgtatt cgcggcgaat ggcaacagtg
     1681 acagccccgg ttttttcagc agcgttttca gtgttctgtt ggttgactct ggcgcgggtg
     1741 agatcaacaa cagacgtgta ttttccggtt tccttgcgtt caccttcgcg acgttttttc
     1801 cagatgcgca tctctgcctg aatttcaggc catttagcgc caggctcaca tttatgctta
     1861 acccacccga tggcatgcag tttaagttca ggatacatgg cgttaacttc tggcattttc
     1921 atcaacgctt caacgatatg tccgccgaat gttgccatgt cttcctgtag taattcctgt
     1981 gcgctaatca ccatatcaac ggtgatgttt tcgcatgtgt cgaatttaac caggactgcg
     2041 ttctgtactt caggggacag cttgtcaaaa ttgacgttca tcggatcgga ttctggttcg
     2101 accggtacaa aggaagctga ctcctcatcc cagcggtttt cctgcatata ttcggtatcc
     2161 caggaatcga gggcagggcg gggtatgccg ggtttatcct cgcagacaat aaatttataa
     2221 gcgcagtcct gagctgccgg gaattgctcc agaaattgcc agtgaaattt tgcgcgtgcg
     2281 cgacgctcat caccggcttc aatggcagtg gccaccgcaa cagcgctatc ttcttttatg
     2341 gcctgttcgt caggaatagc agcgcaaata aagattttac tcattgtgtt ttaacctcat
     2401 tacagatttc agggtgaacg aatccctgcc attgctggca tttttaatcc gttggtatgg
     2461 cgttaatatg gctggcgggt tatccagccg gtgtttcgtt attcaggtac agcgatactt
     2521 tttttaccgg taggcattca ccagaaattt tttgctcgtc tcttgcctgg aggcaggatt
     2581 ctttactggc ataaattccg gtaataacat tctgtgattc acccgttata agaaaaaccg
     2641 tcatcaccag tgcaaatgct gaagtcattg acgttctccg aaaataccaa gtttaataag
     2701 ggcaattcgg gaaagaatgg aattatcatt gagcagataa ggctcatatt tccgcatatt
     2761 aatggcatcc tcagtaaacg ctttattact gagcagaaca ccaatatcaa aacaaccttc
     2821 agacgtatta acgtttggta ataacgtttc cattatcgcg tcctcaacaa tgaattttgt
     2881 gatgcagtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg tcgggttagt
     2941 tgatgctcgt tacgccagta aaagaccgcc ttactgcttt aactgttccg cgtgcgcata
     3001 gccgcattca ccgcatcaca aaattcactt taaaaagggc ggatatccat ttccgccgaa
     3061 tcaccagaaa agtgataaca gaggttgttg tggctggggt gtcacttaag cgtatggtca
     3121 acctgacaac ccggtgtcct caacggggga aggaataacc ccgccatact taccgccgcg
     3181 ccatttcgcg gattgccaca accagaagcg cacggtcgaa ttaaatttaa cgacgaccta
     3241 tacagagaga ctaacttcgc cgtgcgcttt cgtgttatgc cctgactttt cagggtaaat
     3301 taacctgtgg aaacctgttt ttactggcgc taatcagtta gcgtttcttg ctaaccagcg
     3361 atgcgcgcca gcttcggttt taaacgtttt acttctggta tacgtcatcg cggtaaacgt
     3421 gccgtcgtgg ttggggaata ctccgcacac cagagattca ttgttgccaa gattgagcat
     3481 atccatgttg acctcattta cccttaacgc cgggtcgcgg aactaaaaac ctgctgcgct
     3541 gttatacaaa gtgttcccgc cgtcatgttc atacgcctcg ggctggctac ttaacccctg
     3601 accactgccg ggtaactcga ggtattgccc gacgttctgt ggggcgaggt agggtggtat
     3661 gtaataaatc taaaagtatt tagtttttca gtcaaggtaa atctagatta atttaaaaaa
     3721 aaggtcggca ttgccgacct caagtaaagg acgtggcggg ttataagttg aactgtacac
     3781 cttttgctac ggcaacaatt ttacactctg gtgtaagtaa ggatgactga tagcgtggat
     3841 tgagagggct taaatacaca agcttcccat caataactaa tttctttata gtcatagatg
     3901 gttcattttt aagcggatcc ggaactatta cagcgacgat gctgccattt ttataacttt
     3961 ctcctggcct taggatcaca gtagcaccaa ctgggatact cggtgatcct gatgggttat
     4021 gcattgtgtc atcaggcatt gaaacggcaa aatcaccttc ctcgacatca aagaatgtgg
     4081 tgattctatc gacatttccc attgttttct ctccttttaa gattaggaaa gaaatcgcct
     4141 caccccacga aaggtagggg atcttggtgc ctgaggtact ttgtacaaaa gataattcag
     4201 gagacgatac tccatatagg agataggact cagtagttcc taatgcctgg gctaacttac
     4261 ttagagcttt gctacctggt tcgtttagat ctttctccca gtaccctata gtaaccccag
     4321 tcacgcctga aagcttaccc agttctactt gggtcagccc cttatctttt ctaagtctct
     4381 taagcctgaa gccaaggctt tccatcattt tctcctgcaa attgaatata
//
LOCUS       DAZFDC010000223         4365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFDC010000223 DAZFDC010000000
VERSION     DAZFDC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.225"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            166..699
                     /locus_tag="ACHJ48_004658"
     CDS             166..699
                     /locus_tag="ACHJ48_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4710047.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            749..1432
                     /locus_tag="ACHJ48_004659"
     CDS             749..1432
                     /locus_tag="ACHJ48_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4710048.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVTFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSHNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            1445..3895
                     /locus_tag="ACHJ48_004660"
     CDS             1445..3895
                     /locus_tag="ACHJ48_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO4710049.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDKAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLEHGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDITHEGTSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNALQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            3908..4365
                     /locus_tag="ACHJ48_004661"
                     /pseudo
     CDS             3908..4365
                     /locus_tag="ACHJ48_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT         1432 a          859 c          846 g         1228 t
ORIGIN      
        1 tcttataaag aagggaataa ttaagaatat taaattttca aactttcatc aacttcacat
       61 caagaaatta aaaaatttaa acattaatct aaataataac aacctgatca cataatcggg
      121 tttgaaaacg agtgaccttc cataccagtt aattaaggat gatttatgaa aaagtatatt
      181 attcccttcg ttgcgacaac gttgatgttt agcgcaacaa ccttcgccaa ggatgtcgat
      241 caaggaacac taaccattag aggagaagtt gttgatacaa cgtgttactt tgttaatggt
      301 gattcgacaa cagatatcgt tgtcgaagat gttgtaacca gtgcaatgaa tcaacttatc
      361 aataatgaaa tctatacttt aaaaaccggt tcaacctctc atgacttaga aattcagtgt
      421 gacggaaata cagccccaag tatcgaagta ctagcatcag agtttgatgc taataacttt
      481 acgctgaata aaggaactgc tgagaacatt ggctttgcca ttttcctcga tgatgggact
      541 ttgaccagaa tgagaccagg gttaaaaaca ccgctgaaag ataatggctc tggtcaatat
      601 tttctcaatt taaatgcaca atatgcccgc acaagtggca atcctgttac aaaaggctcc
      661 gtagagtcaa ccgtaacgat taaaatcagt gcagattaac gttttttgcc ggtagctctg
      721 ctaccggtat tatttacgga gtgattacgt gagaataata acagcattac tgatgtcatt
      781 ttttatttta cctgttcatg ccggtgtggt catctatggt acgcgcatta tttatccgga
      841 aaaaaacaga gaagttctcg tacagttaat gaatcaaagt aaaaatgcct ctttgattca
      901 ggcatggatc gatgatggga acaccactat cgcgccagaa aaaattcagg tcacatttat
      961 tctcacaccg ccagtaagtc gtgtagcagg tggttctggc caacaactta aaataagaaa
     1021 aatgccaaac aatttgcctc acaataaaga aagtttattt tatctaaatg ttctggatat
     1081 ccctccaaac aatccacaaa atgcagggaa aaacaaaatt aaacttgcat tgcaaaaccg
     1141 gataaagtta ctttggcgtc caagcggaat agctccggta gataaaaaaa gcctttctca
     1201 attaaatatc aaaaagaaaa ataacgccat ctcaataaac aacgaaacag ccaactggat
     1261 aacggttaca actatcaaag cacaaaacgt gaaagttaat aatgaaagta tcttactgcc
     1321 tcccttttcg cataacgaca ttacattaaa aaataaccac gcatcagaat atgaactaac
     1381 ggtagttgat gattacggga ataacatcca tagtaaaatc gccgcgagat aacaggaaaa
     1441 agtcatgtta aggacgacac gatgggtcgc tgctattatt tttttgtatt cttttccggg
     1501 ttatgctgaa gaaacttttg atacgcactt tatgatcggc ggcatgaaag gagaaaaagt
     1561 ttccgaatat catttcgata ataaacaacc gcttccagga aactacgaac ttgattttta
     1621 tgtaaataac cagtggcgtg gaaaacaaga tatcacgatc ccagagtcac cagtcaaacc
     1681 atgtctgcct aaggtgctac taaccaaact gggggtaaag accggtaacc tcaatacaga
     1741 agataattgt attttactgg ataaagcggt tcatggtgga caatatcagt gggatatcag
     1801 tgaacaccga ctaaatttaa cagtaccaca ggcgtatatc aatgaacttg aaagaggtta
     1861 cgttcccccg gaaagttggg atcgcggcat tgatgctttt tatacctcct ataatttaag
     1921 tcagtatcgc tcttatgaca gcaataataa cagtaataca gccagttatg gccgatttaa
     1981 cagtggatta aatttattta gttggcagtt acactctgat gccagctata gcaaacccga
     2041 tgatatgaaa ggcacatggc aaagtaatac tctttacctg gaacatggct ggtcgcagat
     2101 attaagcaca gttcagatag gagaaaatta cacatcctca ctcatattcg actctctgag
     2161 atttagcgga atacgcttat ttcgcgatat gcaaatgcta cctgattcta tgcagagctt
     2221 tacaccttta gtacaaggtg ttgcgcaaag taatgccttg ataacggttt cgcaaaatgg
     2281 ctacatcatt taccaaaaag aggttccccc gggaccattt actatcgcag atttgcaact
     2341 ctcgggcagc ggctctgatc tcgatgtcag cataaaggaa gcagatggca gtgttcgttc
     2401 ctttttggtg ccctactctt ccgtgcctaa catgctgcaa cctggtattt caaatttcga
     2461 ttttatcgcc ggacgcagta agatatatgg tgtaaagaat caggaggact ttctcgaggc
     2521 aaattatata tatggcctca acaatctttt gacactttat ggcggtacga ttttatccga
     2581 taactataat gctataactc tgggaaatgg ttggaataca ccgctgggtg ccatctcttt
     2641 tgacgctaca cgatcgagta gtaagttaaa taatgatata acacacgaag gtacgagcta
     2701 tcaggtcgcc tataataaat atctagtaca aacggccact cgatttagcg tggccgcctg
     2761 gcgttatgct tcgcaggatt acaggacatt cagcgaccat ctttacgaaa atgataaaat
     2821 aaatcatcag agtgactatg acgattttta tgatattggc agaaaaaata gcctttctgc
     2881 caatatcatg caacctttat ccaataatct gggaaatgta tcattaagtg cgctttggcg
     2941 gaattactgg gggcgaagtg gaaatgctaa agattaccaa ttcagttatt ccaatagctg
     3001 gcaacgcatc agttatactt tctctgccag ccaatcctat gatgaaaacg acaaagaaga
     3061 ggagcgtttt aatctgttta tctccattcc tttctactgg ggggatgata ttgccaaaac
     3121 acgtcaccaa attaacttat cgaattcgac ctcattttcc aaagatggct attcctccaa
     3181 caatactgga attactggca tagccggtga acatgatcag ttaaattatg gtatatatgt
     3241 taatcagcaa caacaaaata atgatacatc gcttggtacg aatttaagct ggagaactcc
     3301 catcgccaca atagatggca gctatagcca ttctaaaaac gccttgcaaa gtggtggaag
     3361 tattagtagt ggattagttg tctggcccgg tggtattaat atcactaacc agctatccga
     3421 tacatttgca attctggatg cgcctgggtt agaaggcgcg catattaatg gacaaaaata
     3481 caaccgaaca aacagcaaag gccaggttgt ttacgacctg atgatacctc atcgtgaaaa
     3541 ccatctggta cttgatacag caaacagtga aagtgaaaca gaattgcagg gcaatcgtca
     3601 aattattgcg ccttaccgtg gagcagtttc ttatgtgcag tttacaactg accaacgtaa
     3661 gccctggtat atacaggcac tgcgtcccga tggttcgcca ttaacttttg gctatgacgt
     3721 actggatctc caggaaaaca atattggagt cgttggccag ggtagtcgcc tttttattcg
     3781 cgtagatgaa attccaactg gcataaaagt tgctctcaat gatgaacaga atttattctg
     3841 tactattact tttcaacacg ttatcgatga aaacaaaaca tatatatgcc agtaaaagga
     3901 gtttactatg aagtggcttt tattaatcac attgagtcta tattctttta ttgtgcaaag
     3961 tgctccctgt ggattgacca atgtcgggga acaacgtgga acatatatac tcaaaccact
     4021 gagtatgaaa ggtaatctca ccgccgaaaa aattgaaaat actgggaata tcattgagtg
     4081 tgcagacttg caagagacga ccaacgccat taaagttcat tttttaacac agaacgcttt
     4141 aaggtttaaa gtcggaagtc gtcattatat tattaatttc ccctttgaat ctgacggtaa
     4201 aaaaaatagc gcttccaggg aagtcatctt ataatttaga agaaatgcta tctgcgataa
     4261 atttcaccat tccatactct atcgcatctg tatcagatag ttccaacacc gcagatattt
     4321 ttccaggaac tccttttcct ttgttgttcg aaattgaagt gagtt
//
LOCUS       DAZFDC010000224         4316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFDC010000224 DAZFDC010000000
VERSION     DAZFDC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.226"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            79..663
                     /locus_tag="ACHJ48_004662"
     CDS             79..663
                     /locus_tag="ACHJ48_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4710050.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            743..1438
                     /locus_tag="ACHJ48_004663"
     CDS             743..1438
                     /locus_tag="ACHJ48_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO4710051.1"
                     /translation="MSKRTFAMIFTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLIRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1467..3983
                     /locus_tag="ACHJ48_004664"
     CDS             1467..3983
                     /locus_tag="ACHJ48_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO4710052.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYNFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3994..>4316
                     /locus_tag="ACHJ48_004665"
     CDS             3994..>4316
                     /locus_tag="ACHJ48_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4710053.1"
                     /translation="MKRAPLITGLLLISTSCAYASSEGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKT"
BASE COUNT         1245 a         1006 c          978 g         1087 t
ORIGIN      
        1 ctgtataatc tttaaagatg ttgaacatat attcacatta aatatgatta tgtacttgtt
       61 acaaggataa ggttataaat gaataaagtt acaaaaacag ctattgcggg cttacttgcg
      121 cttttcgcag gtaatgccgc tgcaaccgat ggtgaaatag tttttgatgg cgagatttta
      181 aaatccgctt gtgaaatcaa tgactctgat aagaaaattg aagttgctct tggtcactat
      241 aatgccgaac agtttcgttc tgtaggcgat cgtagcccaa aaattccatt cactattcct
      301 ttagttaact gcccagtgac tgggtgggag cacgataacg gcaacgttga agcgtctttc
      361 cgtttatggc tggaaacgcg ggataatggt accgtcccta acttccctaa cttggcaaaa
      421 gtgggctctt ttgctggcac agcagcaaca ggtgtgggta tccgtattga cgatgcggaa
      481 agcggaaacc ttatgccact aaatgctatg ggcaatgata acacagttta tcagatccct
      541 gcggactccg ctggaattgt caatgttgac ctcatcgctt actacgtatc aaccgttgaa
      601 gcatccgaaa tcaccccagg ggaagcagac gctgtcgtta acgtaacgct ggattaccgt
      661 taatacgtta cggcgttatc tgacctttca gataacgccc ttttccttcc tatttctcgt
      721 tgtatcaggt tgaaaaataa ctatgtcaaa acgaacattc gcgatgatat ttaccttgtt
      781 gtgtagcttc tgtatcggcc aggcgcttgc agggggtatc gttttacagc gaacgcgagt
      841 gatctatgat gccagccgca aagaggctgc gttacctgtc gcaaacaaag gcgcagaaac
      901 accttattta ctgcaatcat gggtagataa tatagatggt acaagccgtg ccccatttat
      961 tataacccca ccgctaattc gtcttgaagc tggcgatgat tcatcactgc gaattattaa
     1021 aacagctgat aacctgcctg aaaataaaga gtcgctgttc tacattaatg ttcgtgccat
     1081 tccagcaaag aaaaaatcag atgatgttaa tgctaacgag ttgacgctgg tatttaaaac
     1141 acggatcaaa atgttttatc gccccgcaca cctgaaggga cgggtaagcg atgcgtggaa
     1201 atcactggaa tttaaacgta gtgatcattc actcaatata tataacccaa ctgaatatta
     1261 cgtcgtattt gccggactag cagtcgataa agccgatctc acaagcaaaa ttgaatatat
     1321 cgcgcccgga gaacataaac agttaccact tccagcatct ggcggaaaga acgtgaaatg
     1381 ggctgcgatc aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca
     1441 aatataaaaa cacaggtcat cagggaatgc cacaacgaca ccaccaggga cataaacgca
     1501 caccgaaaca gttggcgctc attatcaaac gctgtttgcc gatggtgctc actggcagcg
     1561 gcatgctttg cactaccgct aacgccgaag agtattattt cgaccccatt atgctggaaa
     1621 ccacaaaaag tggtatgcaa acaaccgatc tgtcacgttt ttcaaaaaaa tacgcacaac
     1681 taccaggaac ttatcaggtt gatatctggc tgaataaaaa gaaggtttca cagaaaaaaa
     1741 ttacatttac cgccaatgca gagcaacttc tgcagccaca gtttacggta gaacaactac
     1801 gtgagctggg tattaaggtg gatgaaatcc cggcgctggc tgaaaaagat gacgatagcg
     1861 tgatcaactc gcttgaacaa atcattcccg gtacagctgc tgaatttgat ttcaatcatc
     1921 agcgacttaa tttgagcatt ccccaaattg cactgtaccg tgatgcaaga ggttacgtct
     1981 ccccttctcg ttgggacgat ggtataccaa cgctgtttac caactactcg tttacaggtt
     2041 ctgataaccg ttaccgccag ggcaatcgta gccaacgaca gtacctaaat atgcaaaatg
     2101 gtgccaattt tggcccctgg cgattacgta actattctac gtggacacgc aacgatcagg
     2161 cgtcaagctg gaacactatc agtagttatt tacaacgtga tatcaaggcg ttgaagtctc
     2221 agttgcttct gggagaaagc gccaccagcg gcagtatttt ttccagctac aactttactg
     2281 gcgtgcaact cgcttccgac gataatatgt tgccaaacag ccagcgcgga tttgccccaa
     2341 cggtacgcgg tatcgcaaac agtagtgcaa tcgtgactat caggcaaaat ggttatgtga
     2401 tctatcaaag caacgtgcca gcgggtgcct ttgaaattaa cgatctctac ccctcttcca
     2461 acagcggcga tttagaagtc acgattgaag aaagtgacgg tacgcaacgt cgctttatcc
     2521 agccttattc ttcattaccc atgatgcagc gacctgggca tctaaaatat agcgcgaccg
     2581 ctggacgcta tcgcgctgat gcaaacagtg atagcaagga acccgaattt gctgaagcca
     2641 cggcaatata tggtttgaat aatactttta cgctgtatgg cggcctgctc ggttctgaag
     2701 attattatgc gctggggatc ggtatcggcg gcacacttgg cgcactgggc gcgttgtcga
     2761 tggatatcaa cagagctgac acccaattcg ataaccagca ctcttttcat ggctatcaat
     2821 ggcgtacgca gtacatcaaa gatatcccgg aaaccaacac caatatcgct gtcagctact
     2881 atcgctatac caacgatggc tattttagtt ttgatgaagc caatacccgc aattgggact
     2941 ataacagtcg ccaaaaaagt gaaattcaat tcaacatcag ccagacaata tttgatgggg
     3001 taagtctgta tgcctccggt tcacagcaag actattgggg caataacgag aaaaacagga
     3061 atatctctgt tggggtttcc ggccagcaat ggggaattgg ttacagcctg aattatcaat
     3121 acagccgcta cactgatcaa aataatgacc gcgcactctc tttgaatctc agtattccgt
     3181 tagaacgctg gttaccgcgt agccgggttt cctatcagat gaccagccag aaagatcgcc
     3241 caacccaaca tgaaatgcgt cttgatggct cactgctgga tgatggtcgc ctgagctata
     3301 gtctggaaca aagtctggat gacgataaca accataacag tagcgtgaac gccagttacc
     3361 gttcacctta tggaaccttc agtgccggat acagttacgg taatgacagt agccaataca
     3421 attacggcgt taccggcggc gtggttatcc atcctcatgg tgtgacgctc tcgcaatatc
     3481 tgggcaacgc ttttgcgctt attgatgcta acggggcatc tggcgtgagg atacaaaact
     3541 atccggggat tgctactgat ccctttggct atgcagtggt tccttatctc acaacttatc
     3601 aggaaaaccg tctctcggta gatactacgc agctgcccga taacgtcgat cttgaacaaa
     3661 caacacagtt tgtggtgccc aacagaggtg caatggtagc ggcgcgtttc aacgccaata
     3721 tcggttatcg cgtacttgtt acagtcagcg atcgcaacgg taaaccgttg ccctttggcg
     3781 ctcttgccag caacgatgat acggggcaac aaagtatcgt cgatgagggc ggcatactat
     3841 atctctctgg gatatcgagt aaatcacaaa gctggactgt acgctgggga aatcaggcag
     3901 atcaacaatg tcagtttgct tttagtacac cggattcaga accaacaacc tctgtattac
     3961 aaggcacagc gcagtgccat taaggataaa aaaatgaaaa gagcgcctct tataacagga
     4021 cttttgttga tatccacatc ctgcgcttat gcctcctcag aagggtgtgg agctgacagc
     4081 actagcggtg cgacaaatta cagcagtgtg gttgatgatg ttacggtgaa ccagacagat
     4141 aacgtgacag gacgggagtt tacctctgca acgctaagta gcactaactg gcaatacgcc
     4201 tgttcctgct ctgcgggtaa ggcagttaaa cttgtctata tggtcagccc cgtacttacc
     4261 accactggac atcagacagg atattacaaa ctcaatgaca gcctggatat taaaac
//
LOCUS       DAZFDC010000225         4249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFDC010000225 DAZFDC010000000
VERSION     DAZFDC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.227"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            228..347
                     /locus_tag="ACHJ48_004666"
     CDS             228..347
                     /locus_tag="ACHJ48_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO4710054.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            412..>4249
                     /gene="ypjA"
                     /locus_tag="ACHJ48_004667"
     CDS             412..>4249
                     /gene="ypjA"
                     /locus_tag="ACHJ48_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO4710055.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVYGGVSNGSLIESGGYQDVGRHNNYVGQSNNTTI
                     NGGRQSIHDGGISTGTIIESGNQDVYKGGISNGTTIKGGASRVEGGSANGTLIDGGSQ
                     IVKVQGHADGTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIIDNTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNIN
                     SGTQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     ASATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNT
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTL
                     TVNDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLV
                     LAGTEARDSTVGKGGAMQNQGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGT
                     AIVYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLAD
                     LTAASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTT
                     NELSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGG
                     DASFSLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDP
                     KPDPKPDPTPEPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASP
                     HNNNVWGATYNTRNNVT"
BASE COUNT         1265 a          930 c         1108 g          946 t
ORIGIN      
        1 tatatttgcc caacaatggg gggacgaatg ctgtaaaagt aaaacaatct gcgatctcaa
       61 agttattgtg tgatttttat gtgagcagaa gatattcatc agaaacgatt atgtaaatca
      121 ttttattttg ccaacggcct cattgtcgaa agaaaagaat acgcccgtat ttcctgaatg
      181 gattgacttt tttatccaac cacacttcag cgcactgcgt ttaaaaaatg cctcattctt
      241 atgcggaata tcatcatttc atcatgatgt ctttgatgag cggtgaacac aatacacttg
      301 cgctgtctct tcaggatgaa tcccctggtc tggtgcctgt gggctgatgt tgcagcaaag
      361 ctaaggtcgc ttaaacgcta ctcagtattc acttttcaga ggatgaaatt tatgaacagg
      421 accagtccct attattgtcg tcgctcagta ctttccttat tgatatctgc cttgatatat
      481 gccccgcccg ggatggctgc cttcactcct gatgttattg gtgtggtaaa cgatgagact
      541 gtagatggca gccaacgagt agatgaacga ggtacaacaa ataacactca tattatcaac
      601 catggccagc agaatgttta tggcggggta tctaatggaa gtcttattga atctggtgga
      661 tatcaagatg taggaaggca taacaattat gtggggcagt ctaataatac caccattaac
      721 gggggcagac agtcaattca tgacgggggt atttccacag gtacgataat cgagagtggc
      781 aatcaggacg tttataaagg gggtatcagc aatggaacga caattaaggg cggtgcttca
      841 cgcgtagagg gagggagtgc gaatggaaca ctcattgatg gtggtagcca gatagtaaaa
      901 gttcaagggc atgctgatgg tacaacgata aataagtctg gctctcagga cgtagtacaa
      961 ggaagtctgg caacgaacac aaccataaat ggtggtcgac agtatgttga acagagcaca
     1021 gtagaaacaa ccaccatcaa aaatggcggt gagcaaagag tatatgagag ccgtgcgctg
     1081 gacacgacga ttgaaggcgg aactcagtct ctgaatagta agtcaacggc aaaaaatact
     1141 cagatctatt ctggtggtac gcaaattatt gataacacca gctcctcgga tgttattgaa
     1201 gtttattccg gtggcgtgct tgatgttagt ggtggtacgg caacaaatgt tacccagcac
     1261 gatggtgcaa ttttaaaaac taacactaac ggtacgacgg tgagcggtac gaatagtgaa
     1321 ggtgcattct ccatccacaa tcacgtggca gacaatgtgt tgctggaaaa cggtggtcat
     1381 ttagacataa acgcatatgg ttcggcaaac aagacgatta ttaaagataa aggaacaatg
     1441 tcagttttaa ccaatgctaa agctgatgcg acccgaatag ataatggcgg ggttatggat
     1501 gttgcaggaa acgcgacaaa taccataatt aatggtggca cacagaatat taataattat
     1561 ggcatagcca caggcaccaa tatcaacagc ggaacgcaaa atatcaaaag cggcgggaaa
     1621 gctgacacaa caattatatc ctccgggagc cggcaggttg ttgagaaaga tggtacggca
     1681 attggcagca atattagcgc cggaggctcg ctgattgtct ataccggcgg tattgcacat
     1741 ggggttaacc aggagacggg cagtgcttta gttgccaaca cgggtgcagg gactgatatc
     1801 gaaggataca acaagctctc tcacttcact attaccggag gggaggctaa ttatgttgtg
     1861 ctggaaaata ccggcgaact gacggtagtg gctaaaacct cggcgaaaaa tactaccatt
     1921 gatgctggcg gtaagctgat tgtccagaag gaggctaaaa cagatagcac cagacttaat
     1981 aatggcggcg ttctggaggt tcaggacggt ggtgaggcta agcatgttga gcaacaatcc
     2041 ggcggcgcat taattgcttc cacgacctcc ggaacactta tcgaaggaac caacagttat
     2101 ggtgatgctt tctacatcag gaattcagaa gctaaaaatg tagtgctgga aaacgctggc
     2161 tcattaacag tcgtcactgg ttcccgggca gttgacacga ttattaatgc caacggcaaa
     2221 atggatgttt atggaaaaga tgttggcact gtactcaata gtgctggcac ccaaacaata
     2281 tatgccagtg ccacttctga taaagcaaat atcaaaggtg gcaagcaaac ggtatatggt
     2341 ttagccactg aagcaaatat cgaaagtggt gaacaaattg ttgatggtgg gtcaacagag
     2401 aaaacacaca tcaatggtgg cacgcaaacc gttcagaatt atggtaaggc gatcaatacc
     2461 gatatcgtct ctggcctaca acaaattatg gcaaacggga cagcggaagg ttccattatt
     2521 aatggcggtt cacagatagt taatgagggc ggtctggctg aaaactcggt gcttaatgat
     2581 ggcggcacac tcgatgtgcg ggagaaaggc agcgcaacgg ggatacagca gagtagccag
     2641 ggcgcgttgg ttgcaaccac cagggcgacg cgggtcacag gaacacgcgc ggatggcgtc
     2701 gcgttcagca tcgagcaggg tgcggcgaac aatatcctgc tggcaaatgg cggagtgtta
     2761 accgtggagt cagacacctc ttctgacaaa acacaggtca atacgggcgg acgggagatc
     2821 gtcaaaacaa aagccactgc gacaggcacg acgctcaccg gcggtgaaca aattgtcgag
     2881 ggtgtggcga atgagacaac aattaacgac ggcggaatac aaacagtttc agctaacgga
     2941 gaggcaataa aaacaacgat caatgaaggc ggtacgctga cagtcaacga taatggcaaa
     3001 gcgacagata tcgtccagaa cagcggtgcc gctctccaga cgagcacggc taacggtatt
     3061 gaaatcagcg gtactcacca gtacggcact ttttccattt ccggcaattt agcgaccaat
     3121 atgttgctgg aaaatggcgg taatttattg gtattagcag gtaccgaagc tcgcgactcc
     3181 acggttggca aggggggggc aatgcaaaac cagggtcagg actccgccac aaaggttaac
     3241 tctggtgggc aatataccct tgggcggtca aaagatgagt ttcaggctct ggcccgggca
     3301 gaagatctcc aggttgctgg cgggacagca atcgtctacg caggtacgct ggcggatgca
     3361 tcggtcagtg gcgcgacagg aagcctgtcg ttaatgacgc cacgggataa tgttacgcca
     3421 gttaaactcg aaggggcgat ccggattacc gatagcgcga cattaactat cggcaatggc
     3481 gttgatacga cgcttgccga cctgacggct gccagccggg gcagtgtctg gcttaacagc
     3541 aataattcct gtgcaggcac cagcaactgc gagtatagag taaacagttt gctacttaac
     3601 gacggtaatg tttatttatc agcacaaaca gcagcgcctg ccacaactaa cggtatatac
     3661 aatacgctga caaccaatga actttccggt agcggtaatt tctacctgca taccaacgtt
     3721 gcaggctctc ggggcgatca actggtcgtc aacaacaacg ccactggtaa ttttaaaatc
     3781 tttgttcagg ataccggcgt cagtcctcag tctgacgacg cgatgacgct ggtgaaaaca
     3841 gggggagggg atgcttcgtt ttcgctgggc aatactggcg gtttcgttga tcttgggacc
     3901 tatgagtatg tcctgaaaag cgatggcaac agcaactgga acctgaccaa tgatgtcaaa
     3961 cccaacccgg atcccaaccc aaatcccaac ccaaatccga agccggatcc aaaaccagac
     4021 ccaaaaccgg atccgaaacc agacccgact cccgagccaa cgccgacacc cgttccggag
     4081 aaacgcatca cgccttctac cgcagccgta ctcaatatgg cagcaacatt accgttggta
     4141 tttgatgctg agctaaacag tattcgcgag cggttgaaca taatgaaagc gagtccacac
     4201 aacaataatg tctggggggc gacgtataac acccgtaata atgtcacca
//
LOCUS       DAZFDC010000226         4217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFDC010000226 DAZFDC010000000
VERSION     DAZFDC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.228"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHJ48_004668"
     CDS             complement(<1..209)
                     /locus_tag="ACHJ48_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4710056.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHJ48_004669"
     tRNA            complement(324..399)
                     /locus_tag="ACHJ48_004669"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHJ48_004670"
     tRNA            complement(406..480)
                     /locus_tag="ACHJ48_004670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHJ48_004671"
     tRNA            complement(597..681)
                     /locus_tag="ACHJ48_004671"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHJ48_004672"
     tRNA            complement(690..765)
                     /locus_tag="ACHJ48_004672"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHJ48_004673"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHJ48_004673"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO4710057.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHJ48_004674"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHJ48_004674"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO4710058.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHJ48_004675"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHJ48_004675"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO4710059.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGVAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1082 a         1047 c          945 g         1143 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagaca tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc aaccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggcct
//
LOCUS       DAZFDC010000227         4148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFDC010000227 DAZFDC010000000
VERSION     DAZFDC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.229"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(106..1107)
                     /locus_tag="ACHJ48_004676"
     CDS             complement(106..1107)
                     /locus_tag="ACHJ48_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO4710060.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(1113..1460)
                     /locus_tag="ACHJ48_004677"
     CDS             complement(1113..1460)
                     /locus_tag="ACHJ48_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFO4710061.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(1490..2140)
                     /locus_tag="ACHJ48_004678"
     CDS             complement(1490..2140)
                     /locus_tag="ACHJ48_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710062.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHIESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(2156..2560)
                     /locus_tag="ACHJ48_004679"
     CDS             complement(2156..2560)
                     /locus_tag="ACHJ48_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4710063.1"
                     /translation="MKNDIGRRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(2650..2787)
                     /locus_tag="ACHJ48_004680"
     CDS             complement(2650..2787)
                     /locus_tag="ACHJ48_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710064.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            2859..3062
                     /locus_tag="ACHJ48_004681"
     CDS             2859..3062
                     /locus_tag="ACHJ48_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4710065.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            3084..3433
                     /locus_tag="ACHJ48_004682"
                     /pseudo
     CDS             3084..3433
                     /locus_tag="ACHJ48_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
     gene            3444..3722
                     /locus_tag="ACHJ48_004683"
     CDS             3444..3722
                     /locus_tag="ACHJ48_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710066.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGIVPSEKNAATYVYLN
                     GTRLTVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            3734..3976
                     /locus_tag="ACHJ48_004684"
     CDS             3734..3976
                     /locus_tag="ACHJ48_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO4710067.1"
                     /translation="MGKEYKTLINKAPERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            3973..4086
                     /locus_tag="ACHJ48_004685"
     CDS             3973..4086
                     /locus_tag="ACHJ48_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710068.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIIHSVKKIERFFTDK"
BASE COUNT         1246 a         1006 c          768 g         1128 t
ORIGIN      
        1 actatcaacc accatttata aatcataacc ttatgatttt attaactttg aaaatggctc
       61 tatacaacgc catttgaatc tattgtcact ttttatcgcc actttttacc ctctattgtc
      121 aaaagggttc aattcaacag cagcctctaa atgacctgga gcaaaatgcg cataacgcat
      181 agtcattttt atatcgctat gccccagtat tttttgcaac acaagaatat ttccaccccg
      241 catcataaaa tgactggcaa acgtgtgacg tagcacatga gttaattgcc catcaggaag
      301 ctcgatcttc gctctcttaa ttgcagcgtc aaaagcctca taacatggtg aaaatagcgc
      361 tcctcgtttt ttaggaagca tagcttgcaa ttgaggtgaa atcggtacag tgcggttctt
      421 ctttccttta gttttaacaa atgtgattcg accgggcagt acttgagatt gctttaatcc
      481 ttctgcttca ctccaccgag caccagtcgc aagcccaata cggacaacaa cccccaaatc
      541 tttattccgt gactcatcac acgcaatcag aaggcgttca atctcatcta catacagaaa
      601 cgccagttcc ttttcttcct cacgaaactt gcgaatacca gtcagggggt tttcaccaga
      661 ccactcccca aggcgcttca gttcggcaaa aacagcatgt agatatgact gctcgcgatt
      721 aacggttgct tcactaagtt ttttcttccc cttgggattc cattctcctg atagccttct
      781 ttcccgataa gtagcaaaca tatttttgtc aaactgagaa gcaaatggat ctcccagcct
      841 ggaacaaatc gcctcaagtt tgactttgcg ctctgcacca gaggacaagg ttttaccgta
      901 catctcaaac caacgagcaa tcaactcaga aagacgagga ccagaaccat cttgaaactc
      961 gtctccaact ctactattca ttaaacggcg ctcataagag agcgcctcac tttttgtcgc
     1021 aaactgttta cgaatgcgtt ttcccgatgc cccgtaggga taacattcac aaagccattt
     1081 acctgatgga atcttacgaa ccgacatttt agttacttat cacataaatc aaatgcagcc
     1141 ttagtgacat cccccagact cttttttaac cctggggcgg catcattatc tagccaaaat
     1201 ggattattgt tatctaacgg taacgcacca aatgttttac cttttattcg agccaaacct
     1261 gtaagtgcat ataacttatt atcgtcaaaa ttcatcacat aaggattacc atcaagacac
     1321 tgtaattgaa cctcatcagt attaaatggc cataccccat tgaaactctc acgttcaata
     1381 gttttaaaag gcattgcgac ggcggaaaaa gaaaacatag ataaaaaagt aactaatagt
     1441 tgaacctttt ttactttcat atcattacct caatttagct caagtaagat tacaaattaa
     1501 aaaacgccct agaaatgaca ccgcctacca aaacccctac gcagataaag aatattattt
     1561 cttttggata aagtcggatt aattctgaag cacgaagtcg gacttctggt aaggtcgaac
     1621 tctctatgtg gcttgatgcc gattgttgct ctaaccacga caatgcagac tgtaactgag
     1681 aacgagtaag atcgtttaaa cgtcctgtac cgaaattgat atggcaatac cgcagaagtt
     1741 tttgtcgaag tccacagtct tcactgttac gtagtaataa acttacaaga gccttacagg
     1801 catcatgatc tttacatcgc tcaagcattg catgcagaaa actctccgct gttttatatt
     1861 gatttactgt catatcatca ataccagcta caccaatctc cgcatgtact ttttgccaaa
     1921 taataaacgc ttcagtattg ctagcttctg caatagcagc aaccaagcta tttagctcct
     1981 tacgctgagc cttaagcaaa gggcgatcgt catcatcatt attcgaaggg attgcgatat
     2041 tgacggtatg agaaccatca tatcgctcta tctgaatatt cttttcgtga aaatcacgcc
     2101 cagcaacgcg attgtttgaa ccgtttgagt tgacggccat gtcacctccc tactatcacc
     2161 tacccttagt ttcgttatag tcacgaccag cgatacggtt attaccacca gaaatattta
     2221 actcacgtcc tgatggctga gtttcctttt cactgatcgc accttttaaa gccccaatca
     2281 ccgcgttttt cacatctaac gaagctgctc gaaagcgagt aatcaattcc tgctcatcat
     2341 cgttataagt ttcaggtgag tgaattccca acacaacata ctgaacatca aggccaaaac
     2401 gagacagcgc tgccaaatac gcagcatcag gaaagctatc tcctttctca tatctaagct
     2461 gagttagctt tttgactcca ccaatgtcgc tcatggcaac ttgactaagt cccaatcttt
     2521 ccctttcctc acgcaaccgc cgaccaatat catttttcat acaaaaacct tgacaggtat
     2581 cttttttgat accaaaatga tttcacgagc tattagatga tcacaatata ccactatgaa
     2641 acaagttctt cacgatacca gatcacgcat tccgcgtaac accgccacag gtccaagact
     2701 ggcacttcgg ctgtccctcg aggagcgagc cgtcattgat gaaatggcag ctaaagaaca
     2761 acgctcatcc tctaacatgg cgcgcatgat ctcccttcgc ggcctagagc taacccagaa
     2821 agaacaaaac aaatcttcct gatcaggagg ctagtgggat gtcaggtata accatcaata
     2881 tcaatgtgaa tgccccctat gtatccctgc agaaatatgc agagataaca ggtatccctc
     2941 ttaatacatg caaaaagatg ttggctgacg gtcgaattat tatccgcccc aaacgcgcca
     3001 aaatggaaaa gcctgaagta aaccttgtgg cgatgttaaa agacgctttg gctaacagct
     3061 aaaacaatga acagagcacc atcatgaaaa aaaacgctaa taatccatac tccaaatttc
     3121 gtaatggcgt agaacgcatg tacaccacgt cgctaccagt gcatcacgta gtaacagtcg
     3181 ctataacctg aacgagacgc atgcaacacc ggatggccac gctgtaaaac aaatcggcga
     3241 acatgcctgg ctgattgaga aagctggaat cgtggtccac aaatgcccac gcaatccgtt
     3301 taccgggaac cgcatttttg cattgaactg cggcgacaat cacttcgggc aggatttcac
     3361 attatacgaa gcacttcgca cggttgatcg tctgcttcgc gggcaaagtt ttattaaaca
     3421 ggctgattta taacaggtgc tttatgacca aagagcatgc acaaggtgta tttatccgtt
     3481 ttattgattt tcgcggtgaa ctgttattac gcgcatcagc cattgacgga attgttccat
     3541 ccgaaaaaaa cgcagctact tacgtttatc tgaacggtac gcgcctgact gtggagcttc
     3601 cgtaccagac cgtacgagaa atcattagcg aagctgaaaa ggctcgtcag gttaatggcg
     3661 atgaacccta tatcgaaatt atttgtatgg attcagaagc tgaaattcag aaagcagatt
     3721 aaagggcgtt gcgatgggca aagaatataa aactctcatt aacaaagcac ctgaacgatt
     3781 ttattttcgc ttaagtgcat caggcgctca tgctgaacgt gcagcccgtg actcattgac
     3841 cagggcaatc cggagtttgt atgacgttgc tttttacgct gatgatctgg atgcacttaa
     3901 cgaactttcc gagctgatct gtgccgcaga atgcggggaa cacattgaac catataagct
     3961 ggggaatatc gcatgagtat atttatctca tggcttgttc tgattatttc ggtggcctgc
     4021 gccattggga ttatgcgaat tattcattca gtaaaaaaga ttgaacgctt tttcactgac
     4081 aaataacagc ttaaacagaa caccagatta aacccgaaaa cctgaaaact atccgcattc
     4141 gcggaggt
//
LOCUS       DAZFDC010000228         4146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFDC010000228 DAZFDC010000000
VERSION     DAZFDC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.230"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1132)
                     /locus_tag="ACHJ48_004686"
     CDS             complement(<1..1132)
                     /locus_tag="ACHJ48_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO4710069.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTSQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVYHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGDF
                     EFKSKPSYLVLSFAEISSVVFILMSVYMVAKSQNSNGLQYDALFIPSMAFSILVFSFN
                     GGIISKIISNKVMILLGDASFSFYLVHTIVISTLSKFFNVSGLGAISVIKFIVMALFA
                     SLFISIMMYLFFEKPINNKLRKWWSGFKNDVSTTRVEKIEGNQ"
     gene            complement(1142..1822)
                     /locus_tag="ACHJ48_004687"
     CDS             complement(1142..1822)
                     /locus_tag="ACHJ48_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO4710070.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGLQSFATGAIGGAASGAMIGSAVPVIGTGIGALAGGV
                     IGGLGSLF"
     gene            complement(1809..2276)
                     /locus_tag="ACHJ48_004688"
     CDS             complement(1809..2276)
                     /locus_tag="ACHJ48_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO4710071.1"
                     /translation="MEIKLIDNPVKLAEFLNNPVNTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            complement(2276..2812)
                     /locus_tag="ACHJ48_004689"
                     /pseudo
     CDS             complement(2276..2812)
                     /locus_tag="ACHJ48_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
     gene            2876..3523
                     /locus_tag="ACHJ48_004690"
     CDS             2876..3523
                     /locus_tag="ACHJ48_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO4710072.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTKCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAVQASRKDNYKAKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQIINSNKVQTHPLLRMLRRARVRASRAKRESGGYDHYY"
BASE COUNT         1204 a          947 c          912 g         1083 t
ORIGIN      
        1 attgattgcc ttctattttt tcaactcgag tggttgaaac atcattctta aatccactcc
       61 accattttct tagcttattg tttattggct tttcaaaaaa caaatacatc attattgata
      121 taaataatga agcaaacaga gccattacta taaatttaat tacacttata gcaccaagac
      181 cagaaacatt aaagaacttg ctcaatgtac tgattactat agtatggacc aagtaaaatg
      241 aaaaagaagc atcacctaaa agtatcataa ctttatttga tattattttt gaaataatac
      301 caccattaaa cgaaaacact aatattgaaa aggccattga tggtataaaa agcgcatcat
      361 actgtaatcc attgctattt tgactttttg ccaccatata gacagacatt aggataaaga
      421 ctacagatga tatttcagca aatgatagaa ctaaatatga cggctttgac ttaaactcaa
      481 aatctccggc ctgattgctg cggattgtct ctgccagcct gcctcggtca atctcacgac
      541 cagccatctt gtcctgaaca ttgaagtagt caatcggacc gagagcagcc atcccaaggt
      601 gataaacaaa ctcaccaaac cctgaaggat tctgctgata catctgagca acgttgttag
      661 ggtcaacacc gacgcgcgcc agttccttgg cgttgttttg cagccatgat tgcattgctt
      721 ctggagacga ggccgcaagg cgtgcgccag ccgctaaggt gccgatagaa ttgcgctggt
      781 cttcgtctgc ccacttcata ccagactgaa tcttctctaa ttgaccagga tatttggtca
      841 tcagatctcg aacctgctgt cgatcgccgg actggatggc tgccgcatat tctttttgga
      901 atgcagcatc cgcttcctgt tgctttgcgg cttgagatgt ttgagcgaca ctaccaagtc
      961 cctgcaacgc ctgaaggccg atgttattgc gacctgaacg ctccatttcg ttgttctggc
     1021 gaatataggc caacgcctca cttacatcac ttgcctttgg cgcatttgag ttttgcccac
     1081 cgataccagc aagaaaaccg cctgagttga ttccttgttg ccaagtagcc atattcccac
     1141 cttaaaacaa tgatccaaga ccaccgataa caccgccagc aagagcacca ataccagtcc
     1201 caataacagg aactgcacta ccaatcattg cacctgatgc ggccccacca atggcacctg
     1261 tagcaaaact ctgtaggcct gaaggcttat tcgcatttgc tgccgatgct gccgcctgct
     1321 gttgatacaa ttggctgaca ttgttagcgt agttctgtcc ggcgtttgcc tgacctgtaa
     1381 gagcaccaag gccgatattt gccagattgt tgtagttgtt catctgacct gacagccagt
     1441 tttgaccgag tgtaggtgcg attgctgcta actggtttcc tgttgctgta gagcctaatc
     1501 cacccgttgc ctctgctgct gccagactct gatagcgcgc ctgccctgca aggtctttat
     1561 actgctgaga gttgtaatac tggttaagcg cctgaccttg cccctgaaga gaggaaagat
     1621 tctgcaactg tgatacgtac tgctgagcga gtggcgtgaa cggtgcaagg ttttgcatgt
     1681 tcgtctgcca catttcacgc tgcagttcga tgcccttttc agttgcgcgt gcctgggctt
     1741 tagatccgga gtcgctgccg cctttcatat acccattcat gggaagcaat ttattcttga
     1801 agctttcgct aagtactaac atttaatagc tcctcatatt tcgaacgagg taattgatag
     1861 agggtgattc ctaccggttt tccgttactc atgtacgcat catcaaggtg accaacacgg
     1921 gtagcgccaa gcaaacggat aattgcccgt ccgtatttgg tggtgtcagg aaccatggtg
     1981 atgctgttaa ggaatggtga gttttcgaga agccatttgc agaataatcg atgcccttgt
     2041 agtgcatatt cgccacggaa tccggggtca tacaccgcat ggcattcaac aacgctatgc
     2101 cagaagttac gcacttcatg aactccagcc agcactaatc cttcgtagat gccgagatat
     2161 accgcatcag gcttgatgta gtatttatct ccactgtcta cgatattccc cgtgtttacc
     2221 gggttgttaa ggaattctgc aagtttcacc gggttatcga tgagctttat ttccatcact
     2281 gctccgcaat gattttgatg gttgtggcag taaacgccgc gccattcgac tgaatggtta
     2341 acgtgctgcc atttgtggca agaaagccgt ctttatccac gctgaagaac gtagctaaca
     2401 ggatgttgtc ggttgttgtt gccgagttgc gactgcttac tagcgtgtca ggaacagaac
     2461 cggagaatgt tagctgcatt gacctgttgg cggttccgct gggccacgtc ccgacaatcg
     2521 acagcttgaa gaacaaggtt ttgttctcgt tgaacacaac catcttgttg ttaacggtgt
     2581 cgaagaatgg tgccaacgtc ccggatgacg gcgtaagcgt tttcagcagg ctaacaaggt
     2641 tggtcggcgc tgtcgggatg gttactgata cgccagagta aacaacctct gacttcttgc
     2701 gcgtagtggc atactccaga gcattaatgc gcgtttcatg gtctgaaacc tgcgattcca
     2761 gcgactgaac tctggtatca agcgatgcaa tatcgctttc attcaaggca atcagtaggt
     2821 ttacgtcatg gagtgggctc aacaccagtc agagccgcat gtacacgcaa taatcatggt
     2881 gaacgaaaga agcattaatg ggcaagctgg caaaattgtc agcaaatggg taacactggc
     2941 aaacaatgca atcccatcaa atgttcctcc ccagtgggat ctagatttga aaggtcagca
     3001 atattcagag cctacaaagt gtataggtaa acagattgat tacatggcaa agcccgaaac
     3061 taagttaaat gactttaata gagaggcgag caagagccta tatgattggt cagatgccaa
     3121 cgtttggggt tattcgaatg gctgggcgcg gcatgagata aaaaaagaaa cactttctat
     3181 cactggcttt catgctgtaa gacgcatact aaaagccgtt caggcatcaa ggaaagacaa
     3241 ctacaaagct aagcgacaga tacagcgtac attgaaacga caagacataa atcgatcacc
     3301 aatcaccccg ttccaagcac cgttaattag caaacacgat tgggatttat cagtgatagc
     3361 ggtcaccaaa gaggcagaga cagagcgcag ggaattacta tcctcacaga taataaactc
     3421 gaacaaagta caaacccacc cactgttaag gatgttaaga agagcacgag tgagagcatc
     3481 cagagccaaa agagaaagcg gaggttatga ccattactac tagtagtgac agatcgtgac
     3541 aaggcggtgc aaaaaacgac caataataag agagatatgc ctgccaaacc gccaaaagcg
     3601 cggagcgcgg caaacgccgc gagaggcgca agaaggcgaa cctatccatt gaaacggtca
     3661 gaagcgcatc gcgctcgacg accgaagtcc ccactaacac cgttaacgaa agatcaaagt
     3721 cttagaaaaa aatgacgttt ctttgatgga cttccatccg taaaaatcaa attttgagaa
     3781 aaagtactta tagaaattaa tcgctctata aggataagtt gacgttagtt taaagtgata
     3841 aaacattaaa gaaaaaacgt aaaatcaaat acataaaata agtcaccaag ggatcatttt
     3901 cagaaaacaa cgtcaacgta acgtcaactc tgtaagaact tttttggtca ctttttgggt
     3961 tgaatgtacg taaaaatccc cagcagtgaa atttgagcgc gtaagcacaa ttctgagggg
     4021 ctttttgtct gtaggttagg cagtgtaact aacaatacaa gaagttagct ctgactaact
     4081 atttaattaa cttttcaatc taattgcgtt agatattata ccctaacgca atatttttaa
     4141 ttcaat
//
LOCUS       DAZFDC010000229         4144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFDC010000229 DAZFDC010000000
VERSION     DAZFDC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.231"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..185
                     /locus_tag="ACHJ48_004691"
     CDS             <1..185
                     /locus_tag="ACHJ48_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4710073.1"
                     /translation="TPDEQKEPVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPS
                     PELLTMLIRELSGRGR"
     gene            182..532
                     /gene="tnpB"
                     /locus_tag="ACHJ48_004692"
     CDS             182..532
                     /gene="tnpB"
                     /locus_tag="ACHJ48_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4710074.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            563..2148
                     /locus_tag="ACHJ48_004693"
                     /pseudo
     CDS             563..2148
                     /locus_tag="ACHJ48_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2177..2320
                     /locus_tag="ACHJ48_004694"
                     /pseudo
     CDS             2177..2320
                     /locus_tag="ACHJ48_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2431..2738
                     /locus_tag="ACHJ48_004695"
                     /pseudo
     CDS             2431..2738
                     /locus_tag="ACHJ48_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="ACHJ48_004696"
                     /pseudo
     CDS             complement(2735..3970)
                     /gene="pitB"
                     /locus_tag="ACHJ48_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
BASE COUNT         1104 a         1057 c         1093 g          890 t
ORIGIN      
        1 taacgccgga tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca
       61 ctcagaccag agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacatgacgc
      121 tggaaaatcc atcgccagag ctgctcacga tgttaatccg cgaactgagc gggaggggaa
      181 gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgtg
      241 taaatccttc aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc
      301 cggtcacctg tttatcttcc gtggccgacg gggggacacc gttaaaattc tttgggctga
      361 tgctgatggt ctgtgcctgt tcaccaaacg cctggaggaa ggccagttta tctggcctgc
      421 ggtgcgtgac ggtaaggtat ccattacccg ctcgcaactg gcaatgctcc tcgataagct
      481 ggactggcgt cagccaaaaa catcccgcct taacgccctg acaatgttgt aaaaaacacc
      541 tggccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcattg cagaactgga
      601 aagccagctc cgtcagaaag accagcaact gagtctggtt gaagagacgg aggccttcct
      661 gcactggcac gtgccgaaga aaagatcgaa gaagatgaac gggaaataga acatctgcgg
      721 gctcagatag aaaaactgcg ccggatgctg ttcggtaccc gttctgaaaa actgcgtcgt
      781 gaagttgaac aggctgaggc cctgctgaaa caacgcgaac aggacagcga tcgttacagt
      841 gggcgggaag acgatccaca ggttccccgc cagttgcgac agtcgcgcca tcgtcgtccg
      901 ttaccggcac accttccccg tgaaatacag cgtctggaat ctgaagaaag ctgttgcccg
      961 gagtgtggcg gtgagctgga ttatctgggg gaagtcagtg cagaacaact ggaactggtg
     1021 agcagcgccc tgaaagtgat ccgcacagaa cgggtaaaaa aagcgtgtac aaaatgtgac
     1081 tgcatcgttg aagcaccggc gccgtcccgc ccgatagagc gtggtatcgc gggccccgga
     1141 ttacttgccc gcgtgttaac gggaaaatac tgcgaacacc tgccactgta tcgtcagagt
     1201 gaaatctttg cccgacaggg tgtcgaactg agccgtgcat tactctccaa ctgggttgac
     1261 gcgtgctgcc agttaatgac gccgctgaat gatgccctgt accgttatgt gatgaatacc
     1321 cgcaagcttc acactgacga cacaccggta aaggtactgg caccgggcct gaaaaagacg
     1381 aaaacagggc gcatctggac gtatgtccgg gatgatcgca atgcgggttc gtcatctcct
     1441 ccggcggtct ggttcgcgta ctcaccgaac cggcagggga aacacccgga gcaacacctc
     1501 cgccccttcc ggggtatcct gcaggcggat gcgttcacag gttatgacag gctgttcagt
     1561 gcagaacgtg aaggtggtgc gctgacagaa gttgcgtgcc gggcccatgc ccggagaaaa
     1621 atccacgatg tatacatcag cagcaaaagt gcgacggcag aagaagccct gaagcgaatc
     1681 agtgaactgt acgccatcga ggatgaaata cggggattac cagagtcaga gcgtcttgca
     1741 gtcagacagc agcgaagcaa agcgttactg acgtcgctgc atgaatggat gatggagaag
     1801 aatggcacgc tgtcgaaaaa atccagactg ggcgaagcgt tcagctatgt actgaatcag
     1861 tgggacgccc tctgttatta cagtgatgac ggactggcgg agacggacaa taacacagcg
     1921 gaaagagcgc ttcgtgcagt ctgtcttgga aagaaaaatt acgtgttctt cggtagcgat
     1981 cacggcggcg agcgtggtgc actgctatac gggctgatcg gcacctgccg tctgaacggt
     2041 atcgatccgg aagcgtatct gcgccatatt ctgagcgtac tgccggaatg gccctccaac
     2101 cgggttgatg aactcctgcc atggaacgta gtactcacca ataaataagc gtcaatacgg
     2161 tgctccgttg acgcttacct tctttcattc gctgaaggtg gaatgtatcc acggggaacg
     2221 ctttagcagc cgggaaataa tgcgggaaac ggtgtttaat tatatcgagt gtgattacaa
     2281 tcgctggcgt cgtcacagtg cctgtggcgg tctcagcccg gactgtcttg cactgcaaat
     2341 attcagaaat gagtctatgc agttcaccag taaaatcagc catcagagag ggaatgtagt
     2401 ggcacagtaa atttggccac ctgattaaag gtgatatcct caccacaaca caaaacaggt
     2461 gacttaatga acaagaaaac caaacgtact ttcacccctg aattcaggct ggaatgtgca
     2521 cagctaattg ttgataaggg ctactcatat cgacaagcca gtgaagcgat gaatgtcggt
     2581 tctaccacgc ttgagagttg ggtgcgccag ctcaggcgag agcgtcaggg gattgcgccc
     2641 tctgccacac ctattactcc agaccagcaa cgtatccgcg aactggaaaa gcaggttcgc
     2701 cgcctggagg aacacaatac gatattaaaa aaggcttctc accgatggtc atcgcaacac
     2761 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc tactgccatg atgatccaca
     2821 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
     2881 cttctttact gacgcctggc agtttcgcca gcttcgcgga agtatcagag atgcacagca
     2941 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
     3001 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttcccgga tgacagtgaa
     3061 actccgtcac ttgcgcgcca tcagtagatg ccgcaggcaa tggaggttcc atcgcaatca
     3121 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
     3181 gggtacgggt aatttcatag ccggacgcgt tcatattgac gacgaagcca gcaggggcaa
     3241 tccccaccag taccagcatc accaggccga tccctttttg tccgtcgttc gcgccgtgcg
     3301 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggcttac
     3361 gtttgccttt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
     3421 ttccgctcca gtagcgtcgc agcaggaata tcaggcctcc cgcaatgacc aggccgacga
     3481 taggggaaac aatcagcgag gagaaaattt tggtcacttc acgcaggttt aacgcatcca
     3541 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcgccaatca
     3601 aggtatgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
     3661 ccgccagcag catggaaaag accatcgcca ggccgtgggt tgaccccata ttcagcaaca
     3721 aatcggttgg caacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
     3781 caaaaaagtt aaaaaatgcc gccatcacca cggcaagctg tggctgcatg gcacgagtat
     3841 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
     3901 acacaaatgc cagagcaagt aataacaaaa gccctgtgta tatatcaagg ccaacaaata
     3961 aatttagcat atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac
     4021 tttaacggtg tgggcaaagt gaaatatcat tttttaacga taaaaagagt gttatttctt
     4081 gtgtatcaga caataagcat tcattaaaaa tctttagaaa taattagaac acaggcattc
     4141 tgct
//
LOCUS       DAZFDC010000230         4141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFDC010000230 DAZFDC010000000
VERSION     DAZFDC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.232"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            227..835
                     /locus_tag="ACHJ48_004697"
     CDS             227..835
                     /locus_tag="ACHJ48_004697"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO4710075.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVTQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="ACHJ48_004698"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="ACHJ48_004698"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO4710076.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..>4141
                     /gene="selB"
                     /locus_tag="ACHJ48_004699"
     CDS             2321..>4141
                     /gene="selB"
                     /locus_tag="ACHJ48_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO4710077.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDPPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQCLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRD"
BASE COUNT          939 a         1063 c         1204 g          935 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaac ggcgtgacgc aatttaaccc
      361 gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 actcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggttggtg tgtcgaccgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaagcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caaccttggg cgagctttac aggcggaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc
     1321 gcgatcgggc gctggcgcag ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg
     1381 tcaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc ggaaaagagg
     1441 tggtggtatc tcgcggcgaa ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gcttctccgg cgacaagttg ttaggcgggc cgcaggcagg gattatcgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgcgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 cgtgatggca cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt agacgaagcg cgtgttgatg aggttgaacg
     2701 ccaggtaaag gaggttctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt tgccggaacg
     2821 cgagcacgcc agccaacata gtttccgcct cgcgattgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcgc aaaaccagcc
     3001 aacagaaacc gccaatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgtg ccgccagagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtca cgggacgcgt ttcactgctg gaagataacc ttgctgaact
     3241 ggtcttcgac cccccgttat ggctggcaga taacgaccgc ctggtattgc gcgatatctc
     3301 tgcccgcaac acgctggccg gagcgcgcgt cgtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaaacca gagtatctgc aatgcctggc ttccctcgcc agggcacaga atgatagcga
     3421 agcgttagcc gttcacctgc aacgcggcgc ggttaatctt cctgatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaact gctgcaacag cctggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggctg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gatctggcaa aagagacggg taccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga
     4141 c
//
LOCUS       DAZFDC010000231         4134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFDC010000231 DAZFDC010000000
VERSION     DAZFDC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.233"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            120..401
                     /locus_tag="ACHJ48_004700"
     CDS             120..401
                     /locus_tag="ACHJ48_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710078.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQVTQPDSMTADNYVRVVVEVMR
                     GDGIQLNDVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKTPERLRR"
     gene            773..1897
                     /locus_tag="ACHJ48_004701"
                     /pseudo
     CDS             773..1897
                     /locus_tag="ACHJ48_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
     gene            1894..2106
                     /locus_tag="ACHJ48_004702"
     CDS             1894..2106
                     /locus_tag="ACHJ48_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710079.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVIFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            2106..3608
                     /locus_tag="ACHJ48_004703"
     CDS             2106..3608
                     /locus_tag="ACHJ48_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4710080.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            3553..>4134
                     /locus_tag="ACHJ48_004704"
     CDS             3553..>4134
                     /locus_tag="ACHJ48_004704"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFO4710081.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAV"
BASE COUNT          986 a          921 c         1287 g          940 t
ORIGIN      
        1 atgtggttga tgcaaaaaag atgatgattg tggttaattt ttgttcgaca tcttttagcg
       61 tgacagattc tttacaaaaa atctgagctt gttttcttca ccagcgcgat gggggcacaa
      121 tgacagaagc cgaaatactg ggattaatcc gccgcgtcgc cggaatcagc cagcaggttg
      181 acgaacaggt cacgcagccg gacagcatga ccgcagataa ttatgtgcgt gtagtggtgg
      241 aggtgatgcg cggtgatggt atccagctta atgatgtgga tatgcgcaat atacgaacca
      301 gagtccttga gttgctggca taccgtcgcc gttctcaaca acggagggag agcgcgaaaa
      361 atacttacca gtggaagacg ccggaacggc tgcggaggta acttgttgat attcccgata
      421 acgcaaaatt gcgttggctg gtgggtgagt tgcagatctg caactcgatc atgaaattac
      481 ggaaactacc cgtagtttgg gtagtaagag caacacccag attttggggc ttacttgcga
      541 tacccaaata aagggtatcg gtggaagaaa tatcgtttct catatgtgag ttccgagagc
      601 ggaattccgc ctctgatttg tcattgtgat catgcatagc ggcaataatt aatattgctt
      661 ccttttttgc ttttttatcg cttaagcagg tactaaatct gggactatgt tgtagggggc
      721 ataactcaat agtaccttat aatcatttag ttacctttta tttacttctt cttggatgga
      781 ggggccagct gcggcgtttc agacctggga acaactgatt tttaaactgc tggcggcaga
      841 agaagagtat gagcgaaccg gcagtgaaga gaccctgaaa gcggtggtga acaccgatat
      901 cggacgaccc tatctgcccc gttcagccac ggaacagcgt aaaagtgaac tgcttgaaca
      961 gcgtgccgag ccgtttcccc ggcgatctgt gccggatggt gtgcgtttta ttgaggcaac
     1021 ggttgacgta cagggcggta aaaatcgccg ttttgttgtg cagatcaccg gatacggaga
     1081 gcagggggaa cgctggattg ttgatcgcta caacatccgg cattcactgc gctgcagtcc
     1141 caacggtgaa agtctgccgg ttgatccggc ggcatatccg gaggactggg atttgttgct
     1201 gacggatgtg ttccataaaa catggccgct ggcttctgat ccggatgtgc gcatgcgtct
     1261 gatggccatg gcggtggata cgggagggga agccggggtg acagataacg cctatcgttt
     1321 ctggcgtcgt tgccggagtg acggactggg caacagggtg tttctgttca agggggatgg
     1381 acttcgccgt gacaggctga ttaaccgaac cttcccggat aataccggca gaagtgcccg
     1441 ccgtgccaga gccagtggcg atgtcgcgct gtggctggtt cagacggatg cgtttaagga
     1501 tcgtgtaaat aatgccctgt ggcgtgacac accagggccg aactatatcc actttcccga
     1561 ctggctgggg cggtggtttt acgatgagct gacctatgaa gagcgcggca gtgacggaaa
     1621 atggcgaaaa ccgggcaggg gcgctaacga agcgtttgac ctgctggttt atgcggatgc
     1681 gcttgccgtt ctgcatggtt acgaaaagat ccgctggccc tccgcaccgg actgggcaca
     1741 gcgggaaacg tggctcgtct tcccgcagga gcgttctggt gaaacggtat ccccggaact
     1801 gacggccggg gcagaaaaac gccgtcgccg gaagaaaaaa ctgcggacgg agcgtgcgga
     1861 agataatcca tggataacat caggaggctg gttgtgagca cagaagaagc cagagaaatg
     1921 atacagcggt accgtgaagc ggaaatggcc gtactggagg gaaagtctgt catcttcaac
     1981 gggcagcaac tgacgctgga aagcctttct cagatccgcg ccggacgtca ggagtgggaa
     2041 cgcaggcttg ccgcgatggt gagccgcagg cggggaaaac cgggatttaa actggcgagg
     2101 ttttaatggc aattattgat gatgtgatcg gcgtgttttc ccccgggtgg aaagcagcca
     2161 gactgcgttc aagggcgtta atcatggcct atgaggcggt gaaaccgacc cggacacata
     2221 aagcccggcg ggaaaatcgc tctgctgatc agctcagtaa atacggtgcg gtttccctgc
     2281 gggagcaggc ccgttttctg gatatcaatc atgacctggt gattggtgtg tttgacaagc
     2341 tggaagagcg ggtgattggt gccaggggaa ttattgtgga gcctcagcca ttacgaaaaa
     2401 acggggaaat ggcggctgag ctggctgcgg atatccgccg tttgtgggct gaatggtccg
     2461 tgagtccgga tgtgacaggg cagtataccc gtcctgtgct tgaacgttta ctgctgcgga
     2521 cctggctgcg ggatggtgaa gtgtttgcgc agatggtcag tggtgcggga aacggtctgg
     2581 aacggacggc gggagtgcca ttctggcttg aggcgatgga gccggatttt gttcccatgc
     2641 gcactgatga atccgccgga ctgaatcagg gggtttttct tgatgagtgg ggaagaccga
     2701 aaaaatatct ggtttataaa aattatccgg tcagcggccg gcagagtgat acgaaagaaa
     2761 tcgctgccgg aaaaatgatc cacctgaagt tcactcgtcg tctgcatcag acgcgaggct
     2821 catccatgtt atcgggggtg ctgatgcgga tcagtgccct taaggagtat gaggatgcgg
     2881 aactgacagc ggcgcgtatt gctgcggcgc tgggactgta tatccgtaaa ggtgacggac
     2941 aggactatga agatccgggg atcaaagaga ccgagcggga agtccatatc accccgggta
     3001 ttatttatga cgatttgcgc aagggcgagg atatcggcat ggtcaaatct gaccgtccca
     3061 atcccaacct tgaaactttc cgcaacggcc agttgcgtgc agtggcagca ggcagtcgtc
     3121 tgagtttttc cagtgcggcg cgtaactata acggcaccta cagcgcccag cggcaggagc
     3181 tggtcgagtc cacggatggt tacctgatcc tgcaggactg ttttattggc gcggtaaccc
     3241 gcccggtgta ccggacatgg ctgaatatgg tggttgcggc aggtctgctg aaaattccgg
     3301 cggatgtgga gatgaaaacg ctatataacg cgacgtattc cggtccggtg atgccgtgga
     3361 tcgacccggt taaggaagct gaagcctgga gaattcagat ccggggtggt gcagcgacag
     3421 aatctgactg ggtgcgtgcc ggtgggcgca atccggatga ggtcaaacgt cgccgcaagg
     3481 ctgaaattga tgaaaacagc agactggggc tggtctttga tactgacccc gtcaacgaca
     3541 aaggaggcaa cagtgccgga actgaacgac agtatcagcg cgacaccgaa agccagcatg
     3601 aagaataaat cctggttcag gatgcaagct ggggggccgg gtgacgcgga tatttatatt
     3661 tatgacgaga ttggtttctg gggagttacc gcgaagcagt ttgtcagcga actgaatgca
     3721 ctgggtgata tcacccacat taatctccat atcaattcac cgggtggcga tgtctttgaa
     3781 ggcatcgcca tttttaatgc cctgaaaaat cagggggcga ccattaccgt gtatgtggat
     3841 ggcgttgccg cctcgatggc atctgtgatt gcgatggccg gtgatacggt cattatgccg
     3901 gaaaatgcct tcatgatgat ccataagcca tggggattca gtggcgggga tgctgaggat
     3961 atgcgcagtt atgccgattt gctggataaa gtcgaatcgg tactgttgcc agcctatgcg
     4021 cagaaaaccg gaaaaaccac cgatgaaatt gccgccatgc tggcggatga aacctggatg
     4081 tccggtgccg aatgtctggc acacggattt gctgaccagg tgacacccgc tgtt
//
LOCUS       DAZFDC010000232         4122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFDC010000232 DAZFDC010000000
VERSION     DAZFDC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.234"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            159..1262
                     /gene="proB"
                     /locus_tag="ACHJ48_004705"
     CDS             159..1262
                     /gene="proB"
                     /locus_tag="ACHJ48_004705"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO4710082.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1274..2527
                     /gene="proA"
                     /locus_tag="ACHJ48_004706"
     CDS             1274..2527
                     /gene="proA"
                     /locus_tag="ACHJ48_004706"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO4710083.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVVVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIAYSFLPALSKQ
                     MAESGVTLHADASALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2642..2717
                     /locus_tag="ACHJ48_004707"
     tRNA            2642..2717
                     /locus_tag="ACHJ48_004707"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2675..2677,aa:Thr,seq:cgt)
     gene            complement(2732..3652)
                     /locus_tag="ACHJ48_004708"
                     /pseudo
     CDS             complement(2732..3652)
                     /locus_tag="ACHJ48_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3597..3842)
                     /locus_tag="ACHJ48_004709"
     CDS             complement(3597..3842)
                     /locus_tag="ACHJ48_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO4710084.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTNRRIQ
                     MDAQTRRRERRAEKQAQWKADRILASTFRRNATKRHY"
BASE COUNT          939 a         1023 c         1094 g         1066 t
ORIGIN      
        1 attaaatctg taataaaagc gtaaacaact gccgctaggc ttgctgatcc cgcgcaacaa
       61 aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta aaacgttcgt
      121 ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc cagacgctgg
      181 tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgcctgaac cgtgcccata
      241 tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg attgttattg
      301 tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa ctgccagcga
      361 ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt caactgtggg
      421 aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgact cgtgctgata
      481 tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg ctcgataaca
      541 atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt aaagtcggcg
      601 ataacgacaa cctttctgca ctggcggcga ttctggcggg tgccgataaa ctgttgttac
      661 tgaccgatca aaaaggtttg tataccgctg acccgcgcag caatccgcag gcagaactga
      721 ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgctggtgac agcgtttcag
      781 gcctcggaac tggcggcatg agtaccaaat tgcaggccgc tgacgtggct tgccgtgcgg
      841 gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat gtgatggaag
      901 gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct
      961 ggattttcgg tgcgccgcct gcgggtgaaa tcacggtaga tgaaggggca accgccgcca
     1021 ttcttgaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact ggcaacttct
     1081 cgcgtggtga agtcatccgc atttgtaacc tcgaaggtcg cgatatcgcc cacggcgtca
     1141 gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa gaaattgatg
     1201 caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg atcacccgtt
     1261 aaggagcagg ctgatgctgg aacaaatggg cattgccgcg aagcaagcct cgtataaatt
     1321 agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg atgaactgga
     1381 agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg cgcgagccaa
     1441 tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc tgaaaggcat
     1501 tgccgacgat gtacgtcagg tgtgcaacct cgccgatcca gtggggcagg taatcgatgg
     1561 cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg gggttattgg
     1621 cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt gcctgaaaac
     1681 cggtaacgcg gtgatcctgc gtggtggcaa agaaacctgt cgcactaacg cggcaacggt
     1741 ggtggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg tgcaggcgat
     1801 tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat acatcgacat
     1861 gctgatcccg cgcggcgggg ctggtttgca taaactgtgc cgcgagcagt cgacgatccc
     1921 ggtgatcaca ggtggtatag gcgtatgcca tatttatgtt gatgaaagtg cagagattgc
     1981 tgaagccctg aaagtaatcg tcaatgcgaa aactcagcgt ccgagcacat gtaatacggt
     2041 agaaacgttg ctggtgaata aaaacatcgc ctatagcttc ctgcccgcat taagcaaaca
     2101 aatggcggaa agcggcgtga cgttacacgc agatgcatct gcgctggcgc agttgcagac
     2161 aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt ttctgtcatt
     2221 agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata ttcgtgaaca
     2281 cggcacgcaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc agcgttttgt
     2341 taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta ccgacggcgg
     2401 ccagtttgga ctgggtgcgg aagtggcggt aagcacacaa aaactccacg cgcgtggccc
     2461 aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt acaccattcg
     2521 tgcgtaaata aaaccgggtg atgcaaaagt agccgtttga ttcacaaggc cattgacgca
     2581 tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat gtcctttcag
     2641 ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg taggttcgac
     2701 tcctattatc ggcaccattt aaatcaataa gttacacatc attagtacct tccttatttt
     2761 ttgactggga caaatttggg accgatgggt tcaggatcga gtctatttgc cgtgcgtgtt
     2821 cggtaaggtg attaggtgca aggtgagcat atcgacgaac catttcgata gactcccagc
     2881 ctcccatttc ctgtaacact gacaacggga ctccggcttg aaccagccaa cttgcccagg
     2941 tgtgtctcaa gtcgtgaaat ctgaaatcat caataccagc ccgtctcagc gccgctttcc
     3001 aggctgtgtt tgcgtcatac cgcatcttcc ttactgttgg cgctttcgtt ccgtctggtt
     3061 tggtacagct ttccttgtac acaaataccc aacggtgatg attaccgatt tgttttttca
     3121 atacgcgaca tgcagtatca ttcagcgcaa cgccaattgc gcggtttgat ttactctctt
     3181 ccgggtttat ccatgccacc cggcgctgca tatctatttg ttgccattca aggttgatga
     3241 tgttcgagcg tcttaagcct gttgccagtg caaattcaac aacagacttt aatggctccg
     3301 gacattcatc aatcagcctt tgtgcttcat ggggctccag ccagcggagc cgtttattct
     3361 ttggttgagg cactttaata attggtgcct tatcgagcat tttccattca cgctctgcgg
     3421 ctcttagcag ggcctttata aatgaaagat gcgtagcctt cgttgcaacg gacgctggtt
     3481 ttggcgtgta ttctggaaca ggtttccctt tttttctgca tgcttctgcc ctgagtttcc
     3541 agttttcctc atgacgccgg ttcgtcattt tctgcattgc tgaataaatt tttgattcag
     3601 taatgtctct tagttgcatt cctgcgaaat gttgaagcca gaatccgatc tgctttccat
     3661 tgagcctgtt tctctgcgcg acgttcgcgg cggcgtgttt gtgcatccat ctggattctc
     3721 ctgttagtta gctttggttg tgtgtgggag tcgtagtcct gaacgaaaac accccgcaat
     3781 ggcacattgg ctgctaatcc ggattcgcac ttccggccaa tgcttcgttt cgtatcacac
     3841 acaccaaagc cttctgcttt gaatgctgcc cttcttcagg gcttaatttt taagagcatc
     3901 accttcatgg tggttagtgc gtcctgctga tgtgcttagt atcaccgcta gtggtatttg
     3961 tgtcaacacc gccagagata atttatcacc gcagatggtt atctgtatgt tttttatata
     4021 gatttatttt ttgcagggtt gtgtggcttg ggaggtgatc gagagatcag aattgcgttg
     4081 tttagtgagt tgcacctatt aattttaaaa taaatacaat tg
//
LOCUS       DAZFDC010000233         4068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFDC010000233 DAZFDC010000000
VERSION     DAZFDC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.235"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            30..698
                     /gene="ykgH"
                     /locus_tag="ACHJ48_004710"
                     /pseudo
     CDS             30..698
                     /gene="ykgH"
                     /locus_tag="ACHJ48_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
     gene            complement(941..1636)
                     /gene="ykgG"
                     /locus_tag="ACHJ48_004711"
     CDS             complement(941..1636)
                     /gene="ykgG"
                     /locus_tag="ACHJ48_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO4710085.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAKNISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1629..3056)
                     /gene="ykgF"
                     /locus_tag="ACHJ48_004712"
     CDS             complement(1629..3056)
                     /gene="ykgF"
                     /locus_tag="ACHJ48_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO4710086.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGLWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3067..3786)
                     /gene="ykgE"
                     /locus_tag="ACHJ48_004713"
     CDS             complement(3067..3786)
                     /gene="ykgE"
                     /locus_tag="ACHJ48_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO4710087.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1073 a         1058 c          843 g         1094 t
ORIGIN      
        1 tgcggcagat tttattttct ggatatatta tgagtgaaca aatcaaacag gatatcgatc
       61 tgattgagat tttattttat ctgaagaaaa agattcttgt tatccttttt attatagcta
      121 tctgtatggc aatggtgctt ttatttctgt atatcaataa agataatata aaagtgagtt
      181 acagcctaaa aataaatcag acaacacccg gcatacttgt taactgtgat agcaataata
      241 actttgcctg tcagacaaca atgactgaag atgttattca gcgaattact acattttttc
      301 acaccagccc agatgtcaag aacagagaaa taaagctgga atggtcagga gataagagag
      361 atttaccaac tgctgaagcg gaaatatctc gcgtgcaggc gtctattatc aaatggtatg
      421 cgtcagaata ccataatggc aggtaagttc tcgatgagat acaaacgcct tcagcaatta
      481 acagtgagct ttatacaaaa atgatatacc tgaccaggaa ctggtcattg tatccgaacg
      541 gtgatggctg tgtaactata agctcaccag aaataaaaaa taaataccct gctgccattt
      601 gcctggctct gggatttttt ctaagcattg taatttctgt aatgttttgt cttgtcaaaa
      661 aaatggtaga tgaataccaa caaaactctg ggcagtaata aacagcattc tgatagagag
      721 tgcctgtata aagctgaagt gacaatgacc gggaagggaa cccgttttca ggaatggatg
      781 cctgtctttt tcactgtaaa tatcctggca ttagtaatgc caggatattt acagaagagg
      841 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
      901 tatgccttct tcaggtttat gtccagactt catacctctc tcagcaatcc tcaataatca
      961 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atgtccgccg
     1021 ttgagctggg accactgatg atgttaatac atgacggcat gcgttcaccg gcctgcgctt
     1081 tctgatgcaa tctttctgcg agttgcgcta cacgcggcag gatagtgctt ttacgcagga
     1141 taaaaagaga tgattccggg agcaggctca atgaacgccc acgctcggcg gcggaaaaaa
     1201 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
     1261 gctctgcctg cgagatattc ttggcacctt tcgccggatc ccaaacaacg gcattgcatt
     1321 cctgctgcaa acgttcgcta atccctaatt cctccagcct cgtgtcaccg ctaatcacga
     1381 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
     1441 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
     1501 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttattaagc ggcgcatctt
     1561 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
     1621 cgcctcgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
     1681 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
     1741 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcacc ggccatcatc
     1801 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1861 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1921 gacagcggaa tacgcaccgg acacacgttg tcacaagctg tgcataaaga gcaggcgtag
     1981 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     2041 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     2101 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     2161 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     2221 tcgcgaggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     2281 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     2341 atcactgcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     2401 accaggcata ccgaacctgt ctctgccacc gcgaaattac agcccgtaat acctatttca
     2461 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     2521 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     2581 tgaattgccg ggaccacaac atgagagggc ggatcttgat ccagctggag aatatattca
     2641 cccaaatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     2701 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     2761 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa aatagacgtg accgccgttt
     2821 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2881 cgtatctggg cggcccgatc gcgccactcc tcccagagcc ccaattcatc gaccattttt
     2941 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     3001 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     3061 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     3121 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     3181 agggcggact tccatcaggt gcgcaacctt ttctttcacc atctcgccgg atatttcggc
     3241 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     3301 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca cccccagctt
     3361 acgggccagg ctacaagatg ggtgatacac cgctctccct tgcaaactgg cacctacatc
     3421 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     3481 ttcggctcgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     3541 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     3601 cagatttttc atccctggaa tcgcttcttt gatataacca ctattgatcg caggctgacc
     3661 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     3721 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     3781 attcacaact aatactccat tacttcatgc ccatttattc ccgatgtttc cccccacccg
     3841 gggatcaatc atcaataaat atggcgttct tttttaattg gctagcaaca taccacccct
     3901 tatttgtatg acaaatgata gtctttcatc caaacttcaa atgcaataac taattttatt
     3961 tctttcggca tcgctcacat tttatgggga aatcgacgat catttttggc ataagaaatc
     4021 catttaaaaa tggctacatc ccttatacct attcagagct acttattc
//
LOCUS       DAZFDC010000234         4031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFDC010000234 DAZFDC010000000
VERSION     DAZFDC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.236"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..339
                     /gene="cesT"
                     /locus_tag="ACHJ48_004714"
     CDS             <1..339
                     /gene="cesT"
                     /locus_tag="ACHJ48_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO4710088.1"
                     /translation="NDEYMMIYGVCGKFPTDNSNFALEILNANLWFAENGGPYLCYEA
                     GAQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISS
                     SDNKHYYAGR"
     gene            399..3203
                     /gene="eae"
                     /locus_tag="ACHJ48_004715"
     CDS             399..3203
                     /gene="eae"
                     /locus_tag="ACHJ48_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFO4710089.1"
                     /translation="MITHGCYTRTRHKHKLKKTLIMLSAGLGLFFYVNQNSFANGENY
                     FKLGSDSKLLTHDSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSAPLVAAGGVAGHTNKLTKMSPDVTKSNMTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLIYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKSWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTATVKKNGVAQANVPVSFNIVSGTATLGANSAKTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFFDQTKASITEIKADKTTAVANGKDAIKYTV
                     KVMKNGQPVNNQSVTFSTNFGMFNGKSQTQATTGNDGRATITLTSSSAGKATVSATVS
                     DGAEVKATEVTFFDELKIDNKVDIIGNNVRGELPNIWLQYGQFKLKASGGDGTYSWYS
                     ENTSIATVDASGKVTLNGKGSVVIKATSGDKQTVSYTIKAPSYMIKVDKQAYYADAMS
                     ICKNLLPSTQTVLSDIYDSWGAANKYSHYSSMNSITAWIKQTSSEQRSGVSSTYNLIT
                     QNPLPGVNVNTPNVYAVCVE"
     gene            complement(3467..>4031)
                     /gene="escD"
                     /locus_tag="ACHJ48_004716"
     CDS             complement(3467..>4031)
                     /gene="escD"
                     /locus_tag="ACHJ48_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO4710090.1"
                     /translation="VLTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTP
                     GVAGWEIHNNSNNKINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAII
                     NAMNKNSNAKILFQNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGG
                     YVIDAIDPKDGINISRPDEYIHIPLSY"
BASE COUNT         1189 a          778 c          880 g         1184 t
ORIGIN      
        1 aatgacgaat atatgatgat ttatggtgtc tgtgggaaat tcccgacaga taactctaac
       61 ttcgctcttg agattttgaa tgcaaactta tggtttgcag agaatggtgg gccatatctg
      121 tgctatgagg ctggagcaca atcgctgttg ttagcgttac gtttccctct cgatgatgct
      181 acccctgaaa aactcgagaa tgaaatagaa gtcgttgtta agtcaatgga aaacctgtat
      241 ttggtattac ataatcaggg aataacatta gaaaacgaac atatgaaaat agaggaaatc
      301 agttcaagcg acaataaaca ttattacgcc ggaagataaa atccgatcta ttaatataat
      361 ttatttctca ttctaactca ttgtggtgga gccataacat gattactcat ggttgttata
      421 cccggacccg gcacaagcat aagctaaaaa aaacattgat tatgcttagt gctggtttag
      481 gattgttttt ttatgttaat cagaattcat ttgcaaatgg tgaaaattat tttaaattgg
      541 gttcggattc aaaactgtta actcatgata gctatcagaa tcgccttttt tatacgttga
      601 aaactggtga aactgttgcc gatctttcta aatcgcaaga tattaattta tcgacgattt
      661 ggtcgttgaa taagcattta tacagttctg aaagcgaaat gatgaaggcc gcgcctggtc
      721 agcagatcat tttgccactc aaaaaacttc cctttgaata cagtgcacta ccacttttag
      781 gttcggcacc tcttgttgct gcaggtggtg ttgctggtca cacgaataaa ctgactaaaa
      841 tgtccccgga cgtgaccaaa agcaacatga ccgatgacaa ggcattaaat tatgcggcac
      901 aacaggcggc gagtctcggt agccagcttc agtcgcgatc tctgaacggc gattacgcga
      961 aagataccgc tcttggtatc gctggtaacc aggcttcgtc acagttgcag gcctggttac
     1021 aacattatgg aacggcagag gttaatctgc agagtggtaa taactttgac ggtagttcac
     1081 tggacttctt attaccgttc tatgattccg aaaaaatgct ggcatttggt caggtcggag
     1141 cgcgttacat tgactcccgc tttacggcaa atttaggtgc gggtcagcgt tttttccttc
     1201 ctgcaaacat gttgggctat aacgtcttca ttgatcagga tttttctggt gataataccc
     1261 gtttaggtat tggtggcgaa tactggcgag actatttcaa aagtagcgtt aacggctatt
     1321 tccgcatgag cggctggcat gagtcataca ataagaaaga ctatgatgag cgcccagcaa
     1381 atggcttcga tatccgtttt aatggctatc taccgtcata tccggcatta ggcgccaagc
     1441 tgatatatga gcagtattat ggtgataatg ttgctttgtt taattctgat aagctgcagt
     1501 cgaatcctgg tgcggcgacc gttggtgtaa actatactcc gattcctctg gtgacgatgg
     1561 ggatcgatta ccgtcatggt acgggtaatg aaaatgatct cctttactca atgcagttcc
     1621 gttatcagtt tgataaatcg tggtctcagc aaattgaacc acagtatgtt aacgagttaa
     1681 gaacattatc aggcagccgt tacgatctgg ttcagcgtaa taacaatatt attctggagt
     1741 acaagaagca ggatattctt tctctgaata ttccgcatga tattaatggt actgaacaca
     1801 gtacgcagaa gattcagttg atcgttaaga gcaaatacgg tctggatcgt atcgtctggg
     1861 atgatagtgc attacgcagt cagggcggtc agattcagca tagcggaagc caaagcgcac
     1921 aagactacca ggctattttg cctgcttatg tgcaaggtgg cagcaatatt tataaagtga
     1981 cggctcgcgc ctatgaccgt aatggcaata gctctaacaa tgtacagctt actattaccg
     2041 ttctgtcgaa tggtcaagtt gtcgaccagg ttggggtaac ggactttacg gcggataaga
     2101 cttcggctaa agcggataac gccgatacca ttacttatac cgcgacggtg aaaaagaatg
     2161 gggtagctca ggctaatgtc cctgtttcat ttaatattgt ttcaggaact gcaactcttg
     2221 gggcaaatag tgccaaaacg gatgctaacg gtaaggcaac cgtaacgttg aagtcgagta
     2281 cgccaggaca ggtcgtcgtg tctgctaaaa ccgcggagat gacttcagca cttaatgcca
     2341 gtgcggttat attttttgat caaaccaagg ccagcattac tgagattaag gctgataaga
     2401 caactgcagt agcaaatggt aaggatgcta ttaaatatac tgtaaaagtt atgaaaaacg
     2461 gtcagccagt taataatcaa tccgttacat tctcaacaaa ctttgggatg ttcaacggta
     2521 agtctcaaac gcaagcaacc acgggaaatg atggtcgtgc gacgataaca ctaacttcca
     2581 gttccgccgg taaagcgact gttagtgcga cagtcagtga tggggctgag gttaaagcga
     2641 ctgaggtcac tttttttgat gaactgaaaa ttgacaacaa ggttgatatt attggtaaca
     2701 atgtcagagg cgagttgcct aatatttggc tgcaatatgg tcagtttaaa ctgaaagcaa
     2761 gcggtggtga tggtacatat tcatggtatt cagaaaatac cagtatcgcg actgtcgatg
     2821 catcagggaa agtcactttg aatggtaaag gcagtgtcgt aattaaagcc acatctggtg
     2881 ataagcaaac agtaagttac actataaaag caccgtcgta tatgataaaa gtggataagc
     2941 aagcctatta tgctgatgct atgtccattt gcaaaaattt attaccatcc acacagacgg
     3001 tattgtcaga tatttatgac tcatgggggg ctgcaaataa atatagccat tatagttcta
     3061 tgaactcaat aactgcttgg attaaacaga catctagtga gcagcgttct ggagtatcaa
     3121 gcacttataa cctaataaca caaaaccctc ttcctggggt taatgttaat actccaaatg
     3181 tctatgcggt ttgtgtagaa taattccata accaccccgg ctaaaatatg tattgtttta
     3241 gtcggggcat aattatttct tcttaagaaa taacctctta taatcaaatc tactactggt
     3301 ctttttatct gcttaatagg tctctttcaa agagacacat tcacgttttc tcatgtaggt
     3361 tgatccaacc acgctgatac caaagctgaa tcacatcaag caacaactat gctcacaaca
     3421 tccacacaat aaaaaaccct ccgaagaggg ggaagaggat aagaaattaa tacgacagtg
     3481 gaatatgtat atattcgtct gggcgggaga tgttgatacc atctttagga tcaatcgcgt
     3541 ctatgacata gcctcctttc agaatgctac caatccccag gctggtgcca ttatcaagag
     3601 caattgttgg attcttcatc gtaccgctga ttctgagtat ttttccgggg aaaatatctg
     3661 ctgaaatgta cggctgaatg ttctgaaaga gtatcttggc gttactattt ttgttcattg
     3721 catttataat ggcaaggatc ttactctcat tctgttgaga aacttccccc gcaacaatga
     3781 tgacatcatt ctttttgaaa atattgacat agtttattag tttattcttt ttaaactctg
     3841 atgccagttc attgatttta ttgttactgt tgttgtgtat ttcccatcct gcaactcctg
     3901 gcgtattcag taacagattt tcaacctcgc tccatttggg tgaaggggga atgtatcccg
     3961 ataacagaaa gaatccaggc ttattacctt ttgtaataat gatatctttg taaccgtatt
     4021 ccgttaagac a
//
LOCUS       DAZFDC010000235         3959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFDC010000235 DAZFDC010000000
VERSION     DAZFDC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.237"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            113..364
                     /locus_tag="ACHJ48_004717"
     CDS             113..364
                     /locus_tag="ACHJ48_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO4710091.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            399..586
                     /locus_tag="ACHJ48_004718"
                     /pseudo
     CDS             399..586
                     /locus_tag="ACHJ48_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
     gene            complement(1061..1147)
                     /locus_tag="ACHJ48_004719"
                     /pseudo
     CDS             complement(1061..1147)
                     /locus_tag="ACHJ48_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1353..1679)
                     /gene="ynfA"
                     /locus_tag="ACHJ48_004720"
     CDS             complement(1353..1679)
                     /gene="ynfA"
                     /locus_tag="ACHJ48_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO4710092.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGPLIALCGMLIIV
                     VGWGRT"
     gene            1814..2155
                     /gene="ynfB"
                     /locus_tag="ACHJ48_004721"
     CDS             1814..2155
                     /gene="ynfB"
                     /locus_tag="ACHJ48_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO4710093.1"
                     /translation="MKITLSKRIDLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTGNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2190..2750
                     /gene="speG"
                     /locus_tag="ACHJ48_004722"
     CDS             2190..2750
                     /gene="speG"
                     /locus_tag="ACHJ48_004722"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO4710094.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(2753..3463)
                     /gene="ynfC"
                     /locus_tag="ACHJ48_004723"
     CDS             complement(2753..3463)
                     /gene="ynfC"
                     /locus_tag="ACHJ48_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO4710095.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVGCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3571..3876
                     /gene="ynfD"
                     /locus_tag="ACHJ48_004724"
     CDS             3571..3876
                     /gene="ynfD"
                     /locus_tag="ACHJ48_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO4710096.1"
                     /translation="MKLSTCYAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
BASE COUNT         1171 a          939 c          856 g          993 t
ORIGIN      
        1 tatctggcaa aaactgatcc cgagtcaggc tgattcttaa tgttcaactg tcgccggttg
       61 tgactggtat tctgcaaccg gcgctcgtct gatgtaagag ataaagaaat cgatgagcga
      121 agtaatcatg attgtctctc ccggcaaatg ggtatccgaa gagcagttaa ttgcgctgaa
      181 aggaataaaa aaaggtacgt taaaaaaggc ccgggaaaaa tcgtttatgg aaggaaggga
      241 atataagcat gtcgctcatg acggtatgcc atgggataac agtccatgct tttacaacct
      301 ggaagaaatt gatcgctgga ttgagcgcca ggcatcagca agaccaagac gtcatcttac
      361 ttgactaaaa gccacactaa ctaatgagag aagttgaaat gaaatatccg acaggcgtgg
      421 aaaaccatgg agggaaatta cgtatctggt ttgtttataa aggcgtaaga gtcagggaaa
      481 acctggggtt cctgacacag caaaaaacag gcgcgttgca ggtgagctac gcgcctctgt
      541 ttgttacgca ataaaaactg gtgttttcga ctatgcaaaa cagtttccct cctcacgcaa
      601 tctggaaaaa tttggtgagg cccgacaaga tttaaccata aaagaactgg ctgaaaaatt
      661 tctggcactg aaagaaactg aagtcgcaaa aacatcactc aacacatacc gtgccgtcat
      721 caaaaatatc ctgagcataa tcggtgaaaa aaatcttgcc tcatcgatta ataaagaaaa
      781 attactggag gttcgtaaag agctactgac tggataccag atccccaaaa gtaactatat
      841 tgttacacaa ccaaggagat cggctgtaac tgtaaataat tacatgacaa atcttaacgc
      901 cgtgttccag tttggtgttg ataacggtta cctggcagat aacccgttta aggggatctc
      961 gccattaaag gaatcaagaa ccattccgga tcctctttcg cgggaggaat tcatccgtct
     1021 tatcgatgcg tgcagaaatc agcaagccaa aaatttatgg tgtaataccg tcctcagtgg
     1081 tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac aaaaacttca gcctttacga
     1141 tcttcatgtt cgattccttg catcgcttgt cgtgatgcat gaaatctacg caactgagct
     1201 actaccatat aagtatgaag atcgaaaaaa gccggagtga tcacaaaaaa aggcgtatat
     1261 ttgcgctgtg aatggttgac aaaagatgaa atagaatacc ttttgtcagc tgacacttcc
     1321 tcgtatctta ttgataaaat ggatttatgt tcctacgtgc gcccccagcc cacaacaatg
     1381 atcaacatgc cgcaaagcgc aatcaacggc cccgtccagt cataaagagt cagtttcacg
     1441 ccatccacaa cgcgcagcca catcaacgcc gtgcagacat aaacgccacc ataagccgcg
     1501 taaacacgcc cactcgccgc aggatgcaac gttaacaacc agacaaacag cgccagtgaa
     1561 atccccgccg gaagcaacag ccagatactg gcgtttcgtt ttaaccacaa ccagggcaga
     1621 aagcatccaa taatttcaca cagcgcagta gcaaaaaata gtaacgttgt tttaatcatc
     1681 tttgtcactt attgacatca tgtatagtta tagggcgaca taatatcatc aatataaaca
     1741 ccctcctggt acgttttgcg ttcgcagtgg atggtgtaga atcatcttta atcattcata
     1801 cagggaatga attatgaaaa tcactctcag caaacgaatc gacctgctcg cttttctgct
     1861 gccttgcgca ctggcattga gcacaactgt tcatgccgaa actaacaaac tggtgattga
     1921 gtctggcgac agtgcacaaa gccgccagca cgccgctatg gaaaaagagc aatggaatga
     1981 cacgggcaat ctgcgccaga aagtgaataa acgcactgaa aaagagtggg ataaagccga
     2041 cgccgctttt gataaccgcg ataaatgcga gcaaagcgcc aacatcaatg cctactggga
     2101 gccaaatact ttgcgctgcc tggaccgtcg aactggccgc gttattaccc cctaacctgt
     2161 tattgattta aggaatgtaa ggacacgtta tgccaagcgc ccacagtgtt aagctacgcc
     2221 cgctggagcg tgaagattta cgctatgtac atcaactcga caataacgcc agtgtgatgc
     2281 gttactggtt tgaggaaccc tacgaagcct ttgttgaact ctctgatctg tatgataagc
     2341 atattcacga tcagagcgaa cgacgctttg tggtggaatg tgacggcgaa aaagccggtc
     2401 tggtggagtt ggtggaaatt aaccatgtac atcgccgcgc agaatttcag ataattatct
     2461 ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc caaattagca atggactatg
     2521 gcttcaccgt tctcaatctc tataagctgt atctgatcgt tgataaagag aatgaaaaag
     2581 cgattcacat ttaccgcaag cttggctttt cggttgaagg tgaattgatg cacgagttct
     2641 ttattaatgg tcaatatcgt aatgccattc gcatgtgtat attccagcat cagtatctgg
     2701 cagagcacaa aacaccgggt cagactctcc tgaagccgac cgcacaatag cattaataat
     2761 aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc gggttttact ccttcatcaa
     2821 caatgatcag ctgacagccc accggattgg cgtgactgtc atattcacag ctctgtttta
     2881 cattaccaac tcgttgatta ttcagtaaag taaccgctgt gtaatctaat tttttgatcg
     2941 ggtccgttga tggcgtggcg ctgacagata atgttttgtc gttacttttc gtggttttac
     3001 ccagtggata accctgatca tcatagcgat attccatctg catttgtttg ctgctggctt
     3061 taatcacgaa gccattatca tcggtttccc aactcacccc ggcagaaggt aattctgcta
     3121 gctggcattt tccctgtaaa cgtactctct tctccagcgt ctcggcatca cggtaataat
     3181 tggcgtccag taccagagcg accacggtat tattttccag atccagtaat tcgagtgaat
     3241 caaaacagcc ttcttccgac aaagtccccg aaacacgttt cgtcacttca ccttgctcat
     3301 ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag cggatcaaaa tcgaattcat
     3361 tagagaaact ggccatctca ggagtaaatg aaagtgttac ctctgtgcgg tcacatcctg
     3421 tgagtaatat cgcgagtaag catggcagta atttgtattt cacatcagtc accagagagt
     3481 agagatgatt ctcaatcata gtagcaaata cagtacttta cacgtgaaat gctatgctta
     3541 aagaagttat cttcgcgtta ggagcttatg atgaaactct caacctgtta tgccgcgctt
     3601 ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg gatcctgtga gcgcatacaa
     3661 agcgatatat cacagcgcat tatcaataat ggcgtacccg aaagcagctt cacgttaagt
     3721 attgtaccca atgaccaagt tgatcagcct gattcccagg tcgtcgggca ttgcgctaat
     3781 gatacgcata aaattctcta cacccgcacc accagcggta acgtctctgc tcccgcgcag
     3841 tctacccagg atggcgcgcc tgccgaaccg cagtaataca ttatcgacta cacgccggat
     3901 acgacacatc cggcgatttg gacatacaac ataatcccac catattactc atacccttc
//
LOCUS       DAZFDC010000236         3937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFDC010000236 DAZFDC010000000
VERSION     DAZFDC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.238"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..179
                     /locus_tag="ACHJ48_004725"
     CDS             <1..179
                     /locus_tag="ACHJ48_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4710097.1"
                     /translation="AEVIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAE
                     KAYYASIGNNDLAA"
     gene            222..365
                     /locus_tag="ACHJ48_004726"
                     /pseudo
     CDS             222..365
                     /locus_tag="ACHJ48_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
     gene            complement(367..978)
                     /gene="emtA"
                     /locus_tag="ACHJ48_004727"
     CDS             complement(367..978)
                     /gene="emtA"
                     /locus_tag="ACHJ48_004727"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO4710098.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            1078..1992
                     /gene="ldcA"
                     /locus_tag="ACHJ48_004728"
     CDS             1078..1992
                     /gene="ldcA"
                     /locus_tag="ACHJ48_004728"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO4710099.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            2088..3824
                     /gene="cvrA"
                     /locus_tag="ACHJ48_004729"
     CDS             2088..3824
                     /gene="cvrA"
                     /locus_tag="ACHJ48_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO4710100.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
BASE COUNT          860 a          981 c         1051 g         1045 t
ORIGIN      
        1 aagcggaggt aatacaccgt aagagctgga aaaaccggac agaagtggaa ctggccacac
       61 taacgtgggt ggactggtat aacaatcgac gattgctgga aaggctgggc catattcctc
      121 cggcagaagc agaaaaagct tattatgctt ccatcggaaa taatgatctg gcagcctgag
      181 ttcacagata aaatactctc caggaaaccc ggggcggttc agatggcaag aatgaaacca
      241 tcaccactcc ccgtctgagc ttccgttttc ttaacgtcag cccgacggtg gagcggcaat
      301 tacagcggat tattttctct ctcgagcggg aagcccggga gaaagcggac aaagtacgcg
      361 actgatttac atcgcgtcca gtgcctgctc aagtttgtag atatagcgcg gagcctgcgg
      421 cgcaggatga tttcgcgcta cgtgatcgag gaactcgtca gcatccagat cgttgatttt
      481 gctgattgcc tttttccgat ctgacgagaa agtccgtagc agcgcacctg ccccgttagc
      541 atatgacacc accagcgcat attgcagtac cttcggatct tcaatgcctg ccagcgggcc
      601 ggtttccaga atattcaggt aagccgcccc catagaaata ttacgctccg gatttttcag
      661 ctcgctggtc gtcggctcac cactccagcc catacgccga taaacatcac gtccggaggt
      721 tgaagctttt aactgcatca aaccaatggc attcgattta ctcaccgcgt tcggattacc
      781 acccgattcg atagcgataa tcgccgtgat caattgtgga tcgacgcccc aggctgcacc
      841 ggctttctgg cttattggca tccactgcat cgcacgttgc accggaactt tcgcgttcca
      901 cggcgggttc gtatagtcat gctttgatga acaacccgct aataacacaa tcaaaaaggc
      961 aaaccatctc aatttcactt cttctatcct tatagcctgg aatcgccgtt gaggtgacaa
     1021 cggcacacct aagcggcatg atatacattt cgtatctgtt gtcagcaagg aattgccatg
     1081 tctctgtttc acttaattgc cccatcgggt tactgcatta aacagcacgc cgcgctgcgt
     1141 ggtatccagc gccttaccga cgcggggcat caagtcaata acgtcgaggt tattgcccgt
     1201 cgttgtgagc gttttgcggg tacggaaacg gagcgtctgg aagatctcaa ttcccttgcc
     1261 agactgacta cccccaacac catcgtgctg gctgtacgcg gcggttacgg tgccagtcgt
     1321 ttactggcag atattgactg gcaggcactg gtggctcgcc aacaatatga cccgttgctc
     1381 atatgcgggc atagcgattt taccgccatt cagtgcggtc ttctggcgca aggcaatgtc
     1441 atcaccttta gcggccccat gctggtagca aactttggcg cggatgaact gaacaccttt
     1501 accgagcacc atttctggct ggcgttacgc aatgaaacct ttaccattga atggcaaggg
     1561 gaaggcccga catgtcgggc cgaaggcacg ttgtggggag gcaatcttgc gatgctgatt
     1621 tcactgatcg gtacaccgtg gatgccaaaa attgagaacg gtattctggt gctggaagat
     1681 attaacgagc accctttccg cgtcgaacgt atgctgttgc agctctatca tgcgggaatt
     1741 ttgccacgtc agaaggcgat tattctcggt agctttagcg gcagtacgcc taatgattat
     1801 gacgcaggtt acaacctgga gtcagtgtac gcatttttgc gttcccgcct gtcgattcca
     1861 ctcattaccg gcctcgattt tggtcatgaa caacgcacgg ttacgctgcc gttgggcgca
     1921 catgccattc ttactaacac ccgggaaggc actcagttga cgattagcgg tcatcctgtt
     1981 cttaaaatgt aaaaaaaatc acgcttcagg gctaagtaaa acgctgtctc tgccgctaat
     2041 atggtagggt ttatataaga gacagcgtaa tcaggagtaa ccgaccgttg gatgccacaa
     2101 caataattag ccttttcatc ttaggatcca tccttgttac cagcagtata ttacttagtt
     2161 cattttcttc tcgtcttggc attcctattc tggttatttt tctggcgatc ggcatgctgg
     2221 cgggagtcga tggcgtcggc ggtatcccat ttgataatta ccccttcgcc tacatggtaa
     2281 gtaacctggc gctggcgatt attttgctcg atggcgggat gcgcactcag gccagttcct
     2341 ttcgtgtggc gttaggaccg gcgctgtcgc tggcgacgct gggcgtactt atcacctctg
     2401 gtttaaccgg catgatggcg gcgtggctgt ttaatcttga tttgattgaa ggcttattaa
     2461 tcggcgctat cgtcggctcc accgatgctg cagcggtctt ttctctgctg ggtggtaagg
     2521 ggcttaacga acgtgttggc tcgacgctgg aaattgaatc cggcagtaat gatccaatgg
     2581 cggtctttct gacgattacc ctgattgcga tgatccagca gcatgaaagc agtgtcagtt
     2641 ggatgttcgt ggtcgatatt ctgcagcaat ttggcctcgg gattgtcatc ggtcttggcg
     2701 gtggttattt actgctgcaa atgattaatc gcatcgccct gcccgccggt ttatatccat
     2761 tgctggcatt aagcggcggt attttaattt ttgctttaac cacggcgctg gaaggtagcg
     2821 gtattctggc tgtttatctg tgcggttttc tgctgggtaa tcggccgatt cgcaaccgct
     2881 acggcatcct gcaaaatttc gacggcctcg cctggctggc gcaaatcgcc atgttcctgg
     2941 tgctggggct gttggttaac ccaagcgatc tgctgcctat tgccattccg gcgctcattt
     3001 tgtccgcatg gatgattttc ttcgcccgtc ctctttcggt atttgccgga ttgctaccat
     3061 tccgtggctt caatctgcgt gagcgcgtgt ttatcagctg ggtgggatta cgcggcgcgg
     3121 tgccgattat tctggccgtg ttcccgatga tggcggggct ggagaatgca cgtctgttct
     3181 ttaatgtcgc cttctttgtg gttctggttt cactgctctt gcagggaaca tcactctcgt
     3241 gggcggcgaa aaaagccaaa gtggtggttc cgcccgtcgg acgtccggtg tcacgcgttg
     3301 gcctggatat tcatccggaa aatccgtggg agcagtttgt ttatcaattg agtgccgata
     3361 aatggtgcgt gggcgcggca ctgcgcgatt tgcatatgcc aaaagagacg cgtattgcgg
     3421 cactgtttcg tgataaccag ttgcttcatc ctaccggcag cacccgactg cgcgaaggcg
     3481 atgtgttgtg tgtaattggt cgggaacgcg atctcccggc gctcggtaaa ctgttcagcc
     3541 agtcgccgcc ggtcgcgctg gatcaacgct tctttggtga cttcattctc gaagccagcg
     3601 ccaaatatgc agatgtggcg ctgatatatg gcctggaaga cgggcgagaa tatcgtgata
     3661 agcaacaaac gctgggtgaa attgtccagc agttgttagg cgcggcaccg gttgtcggtg
     3721 accaggtaga gtttgccggg atgatctgga cggtggccga gaaagaagat aatgaagtgt
     3781 tgaagattgg cgttcgagtg gctgaagagg aagctgaatc ttaaatttaa tgaagcatct
     3841 atcgccggtt acgatgcttt gcttaacgca gcctgagcct gtatataaac ttgtcatttc
     3901 ctgattttga tatgcacatt ccaacatcag aagaagg
//
LOCUS       DAZFDC010000237         3913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFDC010000237 DAZFDC010000000
VERSION     DAZFDC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.239"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..407)
                     /locus_tag="ACHJ48_004730"
     CDS             complement(<1..407)
                     /locus_tag="ACHJ48_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO4710101.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIA"
     gene            complement(404..754)
                     /locus_tag="ACHJ48_004731"
     CDS             complement(404..754)
                     /locus_tag="ACHJ48_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO4710102.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(765..1091)
                     /locus_tag="ACHJ48_004732"
     CDS             complement(765..1091)
                     /locus_tag="ACHJ48_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4710103.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1088..3510)
                     /locus_tag="ACHJ48_004733"
                     /pseudo
     CDS             complement(1088..3510)
                     /locus_tag="ACHJ48_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(3456..3677)
                     /locus_tag="ACHJ48_004734"
     CDS             complement(3456..3677)
                     /locus_tag="ACHJ48_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710104.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            complement(3722..>3913)
                     /locus_tag="ACHJ48_004735"
     CDS             complement(3722..>3913)
                     /locus_tag="ACHJ48_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710105.1"
                     /translation="EASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRR
                     RTAAVAVITGVNYGSASGG"
BASE COUNT          787 a         1306 c          947 g          873 t
ORIGIN      
        1 gcaatcgcca cctgagctgc ctgttcactg cgcacatcaa acgccgggcg cacaaacggg
       61 tgcggtggca tattcacggt ccccatttcc acaaaccgcc agtagaaagc attgcgcggg
      121 ttatccgcct tcatggtgtt atcgctgtta ccggtgtccg gattaacacc ccggatatgc
      181 acaccggatt ccatcccgcc atcgcgggag cgccgggaaa ggaccaccac attgcggcgc
      241 agttttcccc tgcgtaccgg tgcccgtgac accacttctt ctttcagcac attcgcaccc
      301 gcacgggttg cctcacgcag cacccggtta ttttccgcac cactcagaag ctgcaaatcg
      361 cggctgatgt cctccagccc cgaaaaatcc agcagggttt cgatcatttt tcccctccca
      421 gccgacagag aatttccaga cgcccgccgg tcgcatccgg cacgggcagc ccgacaacgt
      481 tcaggatccg gtcacgccat ggaccactca gcacatgaag tcgtgacgct gccgtgattt
      541 cccggccgga ctgaccgcgc acccagatgc ggatttccgc ctgcgccatt tccgcaccgg
      601 actgcatccg ctcccggctg ctcctgccac ggatatccgc atgaattttc ccgcatgaca
      661 cccattcttc cgtcatttct ccggcagcat tacgggttaa caccgggttc agaacactta
      721 tcatctgtgt cagacgacct gcagatattg ccattcctcc ctcctcataa caccgtcgga
      781 caacgcaaat cgtaaatcag cacggacaca gaaaacggca gttccccctg cacgaggtct
      841 tcccgctcag caagatccgg attccggtac agcatcccgg tcagtcgcat ggcagccccc
      901 ttcatccggg ttaatgcctc gcccgggatc agctcaccgt cctcacgaat cactttatcc
      961 cggctgccct gaatgtaggc cagcagcacg gcggtagcct gacgaacctt gtccatcagc
     1021 atgtcatcat ccgcgtcatg gtcaacacgc agatgtgcct tgatctcttc cagtgtcagt
     1081 aatgccgtca ttttccgcct cctgcatccc gtccacgttt tgcagccagg gtccagcctg
     1141 atgaatgagc ttctccgggt ttatcaccgg tcatactgtt gcagtgccac agcgagcccc
     1201 cccacgtcac cgtatcgccg gggtggtagg ttttaccggc tctgaacaca ccgcggtaga
     1261 gcatcaccgg cagggaaaat gttttttccg tacactggcc actgctctgc cggatcacca
     1321 cagagaacaa ccgctcatcc gtcatgctga cgtcgatatc cgccaccccg tcaaccaggc
     1381 attcccatcc ccgcatcccg tgcgtttttt catacgcccg ccagagtccc cccaggtgtg
     1441 tggcatacgt gccccgggga aaggattttt gatcgtcaat agcggggagc acttccagtg
     1501 ccgtggcatc acgcccgtcc tgcggagccg gaagggcatt caccgcctcc agaaccgcct
     1561 gcttcagtac ttccggatcg taatcacgac catcacgcgg agcagggata tggcttacgg
     1621 cctctttcac catctgctca agcatcggac gcacatcatc gggggtgata cttttgccgt
     1681 ccgccggtac cggaatattc gcaaccgcat cattcaccgc ctgcttcagt acttccggat
     1741 cataatcaca accgtcacgc ggagcaggga tatggcttac agcctctttc accatctgct
     1801 caagcatcgg acgcacatca tcgggggtga tacttttgcc gtccgccggt accggaatat
     1861 tcgcaaccgc atcattcacc gcctgctgca gtacatccgg atcataatca cgaccatcac
     1921 gcggtaccgg aatggtcccc acagcgtcat ccaccatcgc ctgcagaacc ggatgtacct
     1981 catccaccgt cacatgcttc tgtaataccg ccgacaggga agccagtttc tcttcaaacg
     2041 cttgtgcctg cgcggccatc ttcccctcaa atgtgcgctg taaatccgcc agcaccgtgg
     2101 agaattcttc gcccagtgca cgaataatgg acagttcccg ttccgtcatt ttctcagtat
     2161 ccccctgaac atcgctttca ctgcatcatg ctctgtttca ctgattgcct tattaccgtc
     2221 agatgcgccg tcaggcagtt gtgctgaaac tgttttcccg gccgacgcga acgggtcctc
     2281 acgggcatca cgacgggaca gcgcctccag actgtagttc tgctgctgaa gatacagtgc
     2341 atcaccgccg gcaaggggcg gcaggttctc acgtttacgg gcctcattgg gcgtgagaag
     2401 cgtatttttc accgattcac ccagtgtttt catgcgccgt tcgctgtcca ttctcagcag
     2461 cgtggtgacg tcaaactccg tgctctcgtt ttcccccgtt tccagcgcct catccagtaa
     2521 cagctcaatg gactcaatca gcgtctgcag acactgggaa taatactgct gctccagcgc
     2581 ctccacgttg tcactggaag gcgggtggcc aacgccaatc ttgtaggccg ggacacggaa
     2641 caccgaacag acaatttcag cggtcatctt cagttgttcc accgtctgcg catccaccgg
     2701 tgaaaacgtc gtggggttgt attttgcccc gttgctcaaa atggccgttt tccccgcatt
     2761 ttcgccggta tacccgctgt cccagttgct cttcagtttt ttcgcatttt cttccgttat
     2821 actgccgggg atctcaatca ccccggacgg cctgccgcca tttctgaaaa aatacgtcga
     2881 atttgcctga atatgatgcc cctgcatggc ggccagtcct gcggcataca ccggcggcag
     2941 ccccacaagc ggatgaaaaa aacagttaaa acggtcgtgg atcacctccc gggcaggcac
     3001 cgtcaccgac tctgtgatcc cacagttccg gtccggcgtg atgcggtaga acacgtcgcc
     3061 gtcatccgcc accagaggtt caacccggtt ccagtccaga atacgcagtt ctttgatctg
     3121 cccccggggg ttgcggattt tcagcaccac cgtattgccg tgacgcaatt tggaattcag
     3181 ccacagttca aaaaactgga tgcgattctg ctgcgcatta ggacgacgac agagacgggc
     3241 agtatctccc tgccgctttt cccggcgtat tccctgtaca tcagtctgca tcaggcgaag
     3301 ccgcattttg gcaatatcct gggatatcag cgaaatgcat gaaaacaccg cgtgaaagga
     3361 caaaacggtt tccggatcgg ctttcacccc ctgctgccag gcaccggcaa aaggctcagc
     3421 caccgcctga aacagggacc gccagcccac ttctcttacg tcacgtcctg atttctggtt
     3481 ttttcgggtt cgccgcaaaa ggttccacat tcgccatgct ccgcatcacg tttctttttc
     3541 tgacctgccg gacgtcgcgc tgtgatgtac tccgccttcc ccaggcgaac cagcacctcc
     3601 gcacacggct gtgccacatc acggatatcc ccggcccggg catcatgcgt gccctgcaga
     3661 tactggattt ttgccatctg ttactgcggg aagctcgcgc ctcccgccct cctcatcaga
     3721 ctcagccgcc ggacgcactg ccgtagttca ctccggtgat caccgccacc gccgcggtac
     3781 ggcgacgacg ccagttgatc cagcgctccg cacggatggc cacgctgcct gtctggaaca
     3841 tggaaaccag ctccaccggg gacggcgtgg tactgtcgcc ggtcggctca gactgcattt
     3901 ccagtgatgc ttc
//
LOCUS       DAZFDC010000238         3902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFDC010000238 DAZFDC010000000
VERSION     DAZFDC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.240"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..319)
                     /gene="escO"
                     /locus_tag="ACHJ48_004736"
     CDS             complement(<1..319)
                     /gene="escO"
                     /locus_tag="ACHJ48_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFO4710106.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKENLQHVNKSVEKLSFAIKE
                     HYFD"
     gene            complement(379..1719)
                     /gene="escN"
                     /locus_tag="ACHJ48_004737"
     CDS             complement(379..1719)
                     /gene="escN"
                     /locus_tag="ACHJ48_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO4710107.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1703..3730)
                     /gene="escV"
                     /locus_tag="ACHJ48_004738"
     CDS             complement(1703..3730)
                     /gene="escV"
                     /locus_tag="ACHJ48_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO4710108.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSAALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPALVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTN
                     QQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3727..>3902)
                     /gene="cesL"
                     /locus_tag="ACHJ48_004739"
     CDS             complement(3727..>3902)
                     /gene="cesL"
                     /locus_tag="ACHJ48_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO4710109.1"
                     /translation="FFSALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFFLMNTFQQ
                     HVQIIERVRRMTS"
BASE COUNT         1195 a          898 c          712 g         1097 t
ORIGIN      
        1 agtcaaagta atgttccttt atggcaaaag ataatttttc tactgattta ttaacatgtt
       61 gcagattctc ttttgttcgt ttttgtgaat ctagcaatga acgcttttcc ttcgaaagtg
      121 aagatttttt ctcatacaat cgctgctttt gctcatcaaa tgcattgttt ttaatctgat
      181 atttatacaa cgacacttta tccaaattca ccagcgatgc acaatatgcc tgcttagagg
      241 caatcgactc tttaatggat tgctcgcagt catcgagttt accatccacc tcttttatct
      301 gagagttaat cttagccatt gattctctca atcggttcgc tctgctttta cgaatagata
      361 aaattctgtc caacatactc aggcaaccac tttgaatagg ctttcaatcg ttttttcgta
      421 actactgata tctttggtgc tttgttgtat aaagttctga attaatttcc tattttttat
      481 tgccttatct gcttcaggat cttgccccat cgtgtactca ccaatacgaa taagcagctc
      541 aacattttta taagttgcaa taagcttttt gcattctgcc gccgcacgca aatgctccga
      601 tgtaacaacg ttatgcataa cacgactggc acttaaacca atatcaattg cagggaaatg
      661 gttttcctct gcaagttctc gggtaagtac gatgtgccca tcaagaatag agcggacttc
      721 atcgccaata ggatcattaa cattatcgct ttctaacaac accgtataaa tcgcggttat
      781 tgaacctttt ggcgcaggcc ccgctcgctc taataatttg ggtaacgaag aaaaaacact
      841 cggagggaat ccccccctta catcaggttc tcccgatgca agcccaacat cacgtgcggc
      901 tcgggcataa cgagttacag aatccatcat taataataca tttttacctt gatcgcggaa
      961 gtactctgca atagtcgtcg ccgtaaatgc ggctttcatc ctttccagcg cggggcggtc
     1021 tgacgttgtg acgaccagta cacatttaga aagcgtggat tgaggtaaga gcgcgaggaa
     1081 ttcatttact tcgcgaccac gttcgccgat aagagcaagg acaataatat ctgcggatgc
     1141 gccattacaa atcatcccca aaagcgtact tttaccaacg cctgaaccag caaaaatacc
     1201 gatacgctgc ccaataccgc aagttagtag cccatcaatt gccctgacgc caagtataaa
     1261 tggctgatca ataacctgcc ttaataaagg atctggtggt tcagcataca agctgcgttc
     1321 aaacggaaga tagggggcag caatattact ctccattggt ctgcctatgc catcaacaag
     1381 ccgcccaagt agtgcgtctc caacacgaat cttgaactca tctccctgat aacttaacca
     1441 ctgaccacag tacatgccgg aaacatgctc aaaaggcaac aagaaaacct catcttcatc
     1501 aatagcgatc acctcggcta aacgttgact gggttctatc ttataaaagg cgccaatacg
     1561 cgcttttggt aaacgcgcct taataatcgt cccgccgata ttgataatct tgccttcata
     1621 tctggtagac gaatttaatg ataatgccgg cacagtgcta ttgagcactt tctgaatacg
     1681 tgggtatttt tccaatacag aatcatgctc tgaaatcatt taccgttcct aatactttaa
     1741 gttcgatatt attcccaacc tcctgaaaag agagcacggg gactgacggg aacgaacctt
     1801 caatcatttt cttcacatag cgcctgatat ctaaagcggt cagaatgacg ccgttccccg
     1861 ttggattcat gatatttttt aagaagccaa ttatctgctc agttcgctcc ggtgagatat
     1921 tcagatacga acctgatgac gtttgtctga tcgactctcg tagctcattt tcaatatcag
     1981 agccaataag ccaaacgttc agaagtgtac cgctaacgct atagcgacct aaaatatgcc
     2041 gacgcagggc aatacgaacg tattcgcaca agataaccac atccttttct tttgttgacc
     2101 aaaaaataag cgtctcaaag atagttctca ggtctctaat tgagacattt tcctctacga
     2161 gacgttgtag gatgtcaaca attttgctca aaccaagctg gcgctgcagc tctttcacaa
     2221 gctcgttata ttttctctcc atgatgtcca tcaaataacg cgtttcttgt acgccgatga
     2281 actctttggc attagacaat acaagtttct ttagcaaaaa cgttatctta tcatttgttg
     2341 ttgcataaac atccatacct gtaaggtgag cgagctgttg attagtctta ttcgtcttat
     2401 atacgataga gttagtcgaa agcttttcct ggacaaattc gaatgatatc tctgcatgcc
     2461 cagcctcaaa atagacagta tcatctataa gagtatcttt gtatattgac tcttgataga
     2521 gcaaaaattc tattgcgcta tcattatttt taaccggatt aacaataatc ttaggcaatg
     2581 gaattccact ttcctcaaag aaattccaac gcatgacctc aatttgctgt gtaatgtctg
     2641 aactataaat attactactt aagtttaaaa tcaatggctc agcgcccggt gagatgtttt
     2701 ggctattaga caaatcagct cccatagcat ccttattatt gccagttcca aatgttcttt
     2761 tcttctgtaa tttccaggct atccccaaca ggcaaaccgc cagaaataag aagaccaatg
     2821 caggaaagcc cggaataatc gccattacga gcattagtgc ggcactgatt aaaaaagggc
     2881 gactattaac agaaacctgt tgaacaatat cacctgcaag gttttcttct ttttcgcttt
     2941 caccgggcac acgagtaaca accacgcctg cggtgacaga aataataagc gcagggatct
     3001 gggcaaccaa agcatcaccg acagacaata cagaaaacag gctaagtgcc gcactaaatg
     3061 gcatgtcaaa ttgccacata ccaatgagca cgccaccaaa aaggttaact aaaacaataa
     3121 tgataccagc aatagcgtcc ccttttacaa acttcatcgc cccgtccatt gaaccataaa
     3181 actggctttc cttttgcact cgggagcgaa gaacttttgc ctcaagcggg tcaataacgc
     3241 ctgcgcgcat atcaccatca atactcattt gctttcctgg cataccatca agggaaaaac
     3301 gagcactcac ttcagcaaca cgctcggccc cttttgtaat aaccataaat tgaacaatgg
     3361 tgataatagt aaaaataacc aggccaacaa caatattacc gccaacaaca aagttaccga
     3421 aggagtaaat aatgtcaccc gcatcgtgat gcagcagaat aagtctggtt gtactaacac
     3481 tgagcgacag gcgcatcaac gtcgtaatca acaggattgt ggggaaagaa gttagttcaa
     3541 gaggattttt tatatagatt gagagcatta ataaaagtaa agctgtcgaa atattaatcg
     3601 cgataatgat atccaccaca actgttggta gtggaataat catcatcatt acagccataa
     3661 agaagaagag agccagaata agatcgtgat atgactctgc ttttttaaat atatttaaga
     3721 gtttattcat gatgtcatcc tgcgaacgcg ctcaataatc tgcacatgct gttgaaaagt
     3781 attcattaga aaaaaaacat cgatattggt taacaaacaa cttaagcaca atttattatc
     3841 gtagacaaaa atatgttcaa tcactccctg cgtacgctca ggagcaaggg cactaaagaa
     3901 ag
//
LOCUS       DAZFDC010000239         3897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFDC010000239 DAZFDC010000000
VERSION     DAZFDC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.241"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(146..250)
                     /locus_tag="ACHJ48_004740"
     CDS             complement(146..250)
                     /locus_tag="ACHJ48_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710110.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(366..953)
                     /locus_tag="ACHJ48_004741"
     CDS             complement(366..953)
                     /locus_tag="ACHJ48_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4710111.1"
                     /translation="MTTITKDRLLTIQHWRETYGPGSNVVLPAEEAEELARIALASLA
                     AVSDERAAYELFMEKRFGESVDRRRAKNGDRDYMVWDMALGWIIWCHRAAMLQAGNFR
                     ENKGSSTNNFREISETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDG
                     EFFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            complement(956..1147)
                     /locus_tag="ACHJ48_004742"
     CDS             complement(956..1147)
                     /locus_tag="ACHJ48_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710112.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDMPVELKLRNVEIKVK"
     gene            complement(1149..1586)
                     /locus_tag="ACHJ48_004743"
     CDS             complement(1149..1586)
                     /locus_tag="ACHJ48_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO4710113.1"
                     /translation="MGKIDYQALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            complement(1573..1890)
                     /locus_tag="ACHJ48_004744"
     CDS             complement(1573..1890)
                     /locus_tag="ACHJ48_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710114.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYIADEVNDLAILALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     GQD"
     gene            complement(1844..2161)
                     /locus_tag="ACHJ48_004745"
     CDS             complement(1844..2161)
                     /locus_tag="ACHJ48_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO4710115.1"
                     /translation="MNIDPAITIDMALNAGLALLGYFYIMFCSGRWLSLLFMKKWNKR
                     RKQEQRQKAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPH"
     gene            complement(2151..2453)
                     /locus_tag="ACHJ48_004746"
     CDS             complement(2151..2453)
                     /locus_tag="ACHJ48_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO4710116.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKTEEVLKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMLRCVDAVYMLKGWQQSAGAGAELALEEKPGHAVIFQEVGSEY"
     gene            complement(2450..2767)
                     /locus_tag="ACHJ48_004747"
     CDS             complement(2450..2767)
                     /locus_tag="ACHJ48_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4710117.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKCSQRDIELAWQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            complement(2764..3480)
                     /locus_tag="ACHJ48_004748"
     CDS             complement(2764..3480)
                     /locus_tag="ACHJ48_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4710118.1"
                     /translation="METVSDALKALKRASSHVVAARLGISREEAVNELWKLKRRGEAD
                     NKGAIWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGCLIRVNQDGKFRYCIPGVDLPAEPKAASVAETEGKALPQPAGVALPVQETA
                     AQEEIKTEAVEDIVKLQQSFTEAKADDLILPSLHVANRELRRAKSNVQKWERVCAALR
                     ELNKHRDILRDITATREQQR"
     gene            complement(3514..>3897)
                     /locus_tag="ACHJ48_004749"
     CDS             complement(3514..>3897)
                     /locus_tag="ACHJ48_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4710119.1"
                     /translation="LAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYP
                     WPQPVLYHICLEMRDRGIERQMTEGELKRLAERQLTKWAKQVGNGMSIPPIRRQLASP
                     KCPQGPTPIELLKQEYERRKAAGFV"
BASE COUNT          885 a         1158 c          795 g         1059 t
ORIGIN      
        1 aatgttaact ggggcttttc tctgtctgcc ttacggtggc atgcccgagg cagacagcct
       61 caagcacccg cagcaattct acttaactct cgctttaccg caaaccgttt ttacccgata
      121 tgggaattcc catatcgtaa tgaattcagt tccctagtcg atccatcaaa aacacaacca
      181 ggcagtaaac gcccacaaca gcaataacag ccagcgcacc ttccattgcc agtgaaatat
      241 catccgacat attccctcct ttggtgtgaa tcccggcgaa cgtttttacc cccaccgaca
      301 aataacatat actaaaaaat caatagctat agcaacgcct gtaattgcaa aggcttcagg
      361 ccagatcatt ggcgcacctc ctgcggcggt tctggtagcg gcatccagtc ggttacattg
      421 cggctctgtg tttcgaaaaa ttcatcacca ttacggacta catcaaaaaa ctcaccgtct
      481 cgatattgcg cataaagaac gaatgcgcca tcacataaaa taattacgtg ctgaccatca
      541 tccggcattc gctcactaca gcttatccaa ccatccggag ttaccggata gttggttgac
      601 gtttccgaga tttcccgaaa attattggtt gacgaaccct tattttcccg aaagtttcca
      661 gcctgaagca tggcggcgcg gtgacaccag ataatccagc caagcgccat atcccatacc
      721 atgtaatctc tatcgccatt ttttgctctg cggcgatcta cagattctcc gaaacgcttc
      781 tccataaata attcataggc tgctcgttca tccgatactg ctgccagtga tgccagcgca
      841 atccgtgcca gctcttccgc ttcttctgct ggaagcacaa cgttgctacc cggtccgtat
      901 gtttcgcgcc aatgctggat tgtcagcagt cgatctttgg taatagtggt catagctatt
      961 tcaccttaat ctcaacattt cgcagcttta gctctactgg catgtctgac tttcctgtta
     1021 atgctaatgc gagattttct ggagtaatga gagcagttat tgttttcccc atcgccagac
     1081 gaataatcat tcgtatttcg cgatcgtcac atgctcctgg tcgaacaatt gatatttgtc
     1141 cgttcatctc actctccttt gatgcgaatg ccagcgacgc gtaatgcgtg ttctaggtca
     1201 atcaggtaaa gccaactgcc attttcttta ggtatcatga catgtcgctc atctgcattt
     1261 atcgggtgtc catatcgaag gtcgtagcga gtcggtaatt gaacttcccg cgcttccagt
     1321 tcagcaatac gcttgctccc atcagagata acgccttcgt aatactcacg ctgctcgttg
     1381 agttgtgatt ttgcttcttc cagtccatcc agcaaatcag cgataatatc cgcttcccga
     1441 tgacggatgt gacgcttaaa cgcagcaaga gccgcatcac aatcccgttc agcatttggg
     1501 ctgtccgggg tagcctgata ccacgccagc gtcgattgat agttttgtgc tgcctcacgc
     1561 agtgcctgat agtcaatctt gcccactggc tgcctccttt gccgggattt ctaacttttg
     1621 agtggttgta tcaaattcaa acaacttaac cacgtcatca aacaggacat aatcaccatc
     1681 aggatcttca gtcatatcag cgccacaatc ctgaccgcac gagtcgcaac ctcccatatc
     1741 aagctcgtat cgcttcaggt tcgcgatatt tgataaattc agcgccagta tagccagatc
     1801 attaacctcg tctgcgatat accctgcacc atgtccatac atttcaatgc gggatatgat
     1861 ttcttctaca cgttgttttg tgatcgtcat ttttctcttc actccgatat acaaggatta
     1921 ctacaccccc tctgctgatt gcgcgagctg gatcccctgg ttccatgccg tcaattccga
     1981 aggcttcgaa aaatgcatcc attgccttct ggcgttgctc ctgcttacgg cgtttattcc
     2041 attttttcat gaacaacagt gacagccatc gtccgctgca gaacataatg tagaaataac
     2101 caagaagtgc caggccggcg ttcagggcca tatcaatcgt tatcgccggg tcaatattca
     2161 ctgcccacct cctgaaaaat caccgcatgg cccggtttct cctccagtgc cagctcagcc
     2221 cctgcacctg ccgactgctg ccagcctttc agcatgtaaa ccgcatccac gcaacggagc
     2281 attgccatgc aaatatccat gtagtgtggc tgagtcagcc cgtccggaag tactgccggg
     2341 tttaaaacgg tatgcccttc ccgtttcagc acctcttccg ttttgtgaaa tgcctcgcgg
     2401 ttaaaatttt catatcccgt catcggaccg gcgatataaa ccctcaccct cactcctgaa
     2461 ccctcctgtc gaaataaacg tagttattca ctgcgcccaa cttcatccca aacttttcgg
     2521 caatttcccg tcggggtaca ccacgctgat gcagttgcca cgccagctca atatcacgct
     2581 gtgaacattt ggctgactgg tgataatcac cccgtaacat catgctgata cccagttccc
     2641 gcgctttcgt cctgacggct gactcactac ggccaatcag ataaccgatg ctttcgactc
     2701 tcatcgttcc cgcacactgc cggagtatca ggatttcagc ccagcgccac ttcttccagc
     2761 cactcaccgc tgctgctctc tggtggcggt aatatcccgg agaatatccc tgtgtttgtt
     2821 cagttcccgt agcgcagcac agactcgctc ccacttctga acattacttt tcgcccggcg
     2881 cagctcgcgg ttagccacat gcagcgatgg tagaatcagg tcatctgctt tcgcttcagt
     2941 gaacgattgc tgcaacttca caatgtcctc caccgcttct gttttaattt cttcctgtgc
     3001 agccgtttcc tggactggta acgcaacacc agctggctga ggaagggctt taccctccgt
     3061 ttcagctacg gatgctgctt tcggctctgc cggtaaatca acgcccggta tgcagtaacg
     3121 aaatttacca tcctgattca cgcgaatcag acaccctttg ctgattgcca ttgccagcga
     3181 tgaattcgcc cggcgggagg taattccgaa catcaatgcc agttcatccg ccgtttgtgg
     3241 gccatgttgt tcaatcgcct ctgtcagcat ttgcgccgtc actttcggta ccggtgacac
     3301 cggttcactt tcaccagcct gagtcagcca ccatatcgcc cccttgttat ccgcttctcc
     3361 acggcgtttc agtttccaca gttcgttgac agcctcttca cgactaattc caaggcgagc
     3421 tgccaccaca tgtgaagagg ctcttttcag tgctttcagt gcgtcagata cggtttccat
     3481 taaaatttcc tccgacaaaa tcgtttctca gattcaaaca aaaccagccg ccttccggcg
     3541 ctcgtattcc tgtttcagta attcaattgg cgttggccct tgcgggcact tcggcgatgc
     3601 gagttgtctc cggatcgggg gaatactcat cccgttacca acttgctttg cccatttcgt
     3661 cagttgccgt tctgcaagtc gttttaactc accttccgtc atctgccgct caatccctct
     3721 gtcacgcatc tcgaggcaaa tgtggtacag caccggctgt ggccacgggt atttatcact
     3781 cccgtcgtac cgccaggatt cattcctcca gcgacggtac tcctccatca cggcatccac
     3841 cgtcaggcca aatggatttg ccccgctctc cgaaatcaac gccacaaact cagccag
//
LOCUS       DAZFDC010000240         3890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFDC010000240 DAZFDC010000000
VERSION     DAZFDC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.242"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(88..1245)
                     /gene="intS"
                     /locus_tag="ACHJ48_004750"
     CDS             complement(88..1245)
                     /gene="intS"
                     /locus_tag="ACHJ48_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO4710120.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKRQVWSAAYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLAEAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIEASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKSICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(1407..1481)
                     /locus_tag="ACHJ48_004751"
     tRNA            complement(1407..1481)
                     /locus_tag="ACHJ48_004751"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1446..1448),aa:Arg,seq:cct)
     gene            complement(1557..2489)
                     /gene="yfdC"
                     /locus_tag="ACHJ48_004752"
     CDS             complement(1557..2489)
                     /gene="yfdC"
                     /locus_tag="ACHJ48_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO4710121.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2783..3538
                     /gene="mlaA"
                     /locus_tag="ACHJ48_004753"
     CDS             2783..3538
                     /gene="mlaA"
                     /locus_tag="ACHJ48_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO4710122.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(3720..>3890)
                     /locus_tag="ACHJ48_004754"
     CDS             complement(3720..>3890)
                     /locus_tag="ACHJ48_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710123.1"
                     /translation="VSTLVGNKVNVGHSQKIYKHFKDLEGKTEERLQNRQAEYQNEIN
                     QLYALGVNFDDI"
BASE COUNT          954 a          933 c          898 g         1105 t
ORIGIN      
        1 tggtttcttg gtgtaagaaa tcctgagaag tgaaaagctt tgctatatgt gcaaggcttt
       61 tcttttttat aagaatcgtt gtaataacta ctccaccttc ccatcaagcc agtccgccca
      121 ccactgcatc atttctctgc gcttatcgag atactgagca tggttgtaaa tcccacgcac
      181 agatccgccg ttggcatgtg ctagttgcac ttcaatagcg tcagcaggcc attcgtgctc
      241 gttcataatc gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc
      301 gatttgtttg atcacaagca gtacagcgtt ctcacagatt gacttcttct tatcgttgcg
      361 cccggcaaaa acaaactctg atactggttt agtgatggag cttagcgtag tgagaagttc
      421 aacaacctgg tccgacatcg gaaccacatg aatcttgcgt cccttcatca cactggcctc
      481 gatggtgata atcctgtttt caaaatcgac gttcttccat tgcatggaac gaagctcttt
      541 cgttcttagt gctgtgtagc gtaaaacttt ggtcgcaatg agagatacga tacttcctga
      601 aaatgttgca agtgctttgt tgaatgccgg gatctggtcg gcaggtaaaa acgggaagtt
      661 cttcttgcgg tatcccttca tggcttcagc aaggtcaggt gccgggttat atttagccct
      721 tccggtgaca atagcgtaac ggaaaacctc gccgcatctt ctgcgtgctt tgttggctcg
      781 ctccattgcc ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat
      841 atcctgaatt tcaaggccgc cgatgatagg taaaatgtcg tcatcaaaca tttttgcaag
      901 ttcagtcgca taggcggctg accagacttg cctcttatgc tcgtaccatt ccttgtaaat
      961 cgcactaaag gaattgttgt tagacgaagc ctttttcgcc tttaccggat cgatgccaac
     1021 cgagatgtct ttcctcgcag tccatgcttt atcccttgcc tcttgcaaag tcataagcgg
     1081 atattttccg acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac
     1141 ctttttccct gatacaggga cataaaggta caggccatta ccatcgagta ggcggtatgg
     1201 tttttctttc ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt
     1261 gggtaaaatt attttatcca ctttttaccc gtcatggcgt gcggctgtca acgatctgac
     1321 gcgaaccatg acgaactgtg aatctacgga aggcttgata ttcaggggat tttgcggact
     1381 ggtacggatg gaggcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag
     1441 cccttaggag gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc
     1501 cgctaatgga ctgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat
     1561 gccgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct
     1621 gctttgcgct tgttgctcat gtcgttacgg atctgtgcat gactcattaa cgcgaagata
     1681 aaggtgccgc cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg
     1741 ctccagtgca gcgtaccgtt aaacaccaga tagaggattt caacagaacc gacaacgatg
     1801 tgagtggtgt cacccagggc aataagccag gtcatcaata taatcaccac aatctttgcc
     1861 gcacccgctg caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg
     1921 ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca
     1981 tcgcgagttt cttcattgaa gattggcata tattcaaatg cccacgccgc aatacctgtc
     2041 ccgagaatat tacccagcag cacgacgccc cataaccgca taagtaagcc aacgttgctc
     2101 attgtcggtt tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc
     2161 tggcgggcca taatgacgat aataaaacca aaggtataac cgagattctc cagcaagaag
     2221 ctgcccggca caccttccag ttcgacatga aatatccctt ttgccagtaa tgaagcgccc
     2281 atcgacagac ccgccgcaat ggctgaccac agtagtgcca ttgcgtcgcg ttccagctct
     2341 ttttcaccat cctggcggat atgctcatga attgccatcg cccgggaggg gagtcgatct
     2401 tcatctattt ctattttttt gccacgctct ttttcttcgc tctcaacttc aatttcgtcg
     2461 ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct
     2521 aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctttgaaaat gtagaaaagg
     2581 ccgcgtttgc ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag
     2641 gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg
     2701 cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc
     2761 ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac
     2821 gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga
     2881 agggttcaac cgcaccatgt acaactttaa cttcaatgta ttagacccgt atattgttcg
     2941 accggtcgct gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaacg gtttgagcaa
     3001 ttttaccggc aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc
     3061 ttatcagggg atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg
     3121 ctttattgat gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt
     3181 cggtagtacg cttggtcatt atggcgtggg ttatggcccg tacgttcaat taccgttcta
     3241 cggtagcttc acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct
     3301 ttcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggta tcgaaacccg
     3361 cgcacagctg ctggattccg acggtctgct gcgtcagtcg tccgatcctt atattatggt
     3421 gcgcgaagcg tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca
     3481 ggaaaatccg aacgcgcaag cgattcagga tgatttaaaa gatatcgatt ctgaataaga
     3541 aacaaataaa aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca
     3601 caagtaaaag gagataaacc gacaatgacc cggacgtaat tatgtaaaaa tactgatgtt
     3661 cgtcactgac tatttccgat attcagctgt tgaaaaatca aaaaaatgga aaaataattt
     3721 taaatatcat caaaattaac acctagcgca tatagttgat taatttcatt ttgatattca
     3781 gcctggcgat tttgaagcct ttcttcagtt ttaccttcga gatccttaaa atgtttatat
     3841 attttttgag aatggccaac gttaactttg ttaccgacta aagtagaaac
//
LOCUS       DAZFDC010000241         3883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFDC010000241 DAZFDC010000000
VERSION     DAZFDC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.243"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHJ48_004755"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHJ48_004755"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO4710124.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACHJ48_004756"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACHJ48_004756"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO4710125.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          988 a         1025 c          981 g          889 t
ORIGIN      
        1 gataaaaaaa ttaaaggcaa ttattctccg ataaagacgc tggttaacag agtacaggct
       61 cgtttattga tgagcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga aaccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ctgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 gacgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatctcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gttcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagtc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg acaactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacgctaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaagatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaatcacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat cgctgaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ctttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg tcgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc gatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtac tgttggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgctc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactctatc
     3421 ctgttgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggaactg
     3541 ggtaaacagc tggcgaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatcg
     3661 ttcatgcctg atgccgctat gtaggccgga taaggcgttt acgccgcatc cggcaactgg
     3721 tgcctgatgc gacgctttac gcgtcttatc aggcctacag gtcgatgccg atatgtacat
     3781 cgtattcggc aattaatgca tagcacgatt aattaaataa ccttaaaaac aatgccgact
     3841 ataagtcggc attgttttat cagataaatc cccttgtctg tga
//
LOCUS       DAZFDC010000242         3832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFDC010000242 DAZFDC010000000
VERSION     DAZFDC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.244"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..181
                     /locus_tag="ACHJ48_004757"
     CDS             <1..181
                     /locus_tag="ACHJ48_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFO4710126.1"
                     /translation="WLLESPSRVSIVEDKHIYYFHLLKDFFTSLPDACFIDSEQRENA
                     LLMIGKVIDYKEEII"
     gene            240..818
                     /gene="espA"
                     /locus_tag="ACHJ48_004758"
     CDS             240..818
                     /gene="espA"
                     /locus_tag="ACHJ48_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO4710127.1"
                     /translation="MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQA
                     AILMFSYMYQAQSNLSIAKFADMNEASKASTTAQKMANLVDAKIADVQSSTDKNAKAK
                     LPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKAAISAKANNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDVQSLQYRTISAISLGK"
     gene            831..1955
                     /gene="espD"
                     /locus_tag="ACHJ48_004759"
     CDS             831..1955
                     /gene="espD"
                     /locus_tag="ACHJ48_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspD"
                     /protein_id="HFO4710128.1"
                     /translation="MLNVNNDTLSVTSGVNTASGTSGITQSETGLSLDLQLVKSMNSS
                     AGWTESSPLPTPPAGHSLVTPSAAEDVLSKLFGGISGEVTSRTEEAEPQRTSYPYLSQ
                     VNTVDPQQMMMMVTLLSLDTSAQKVSSLKNSNEIYMDGQTKALENKTQEYKKQLEEQQ
                     KAEEKSQKSKIVGQVFGWLGVALTAVAAVFNPALWAVVAIGATAMALQTAVDVMGENA
                     PQGLKTAAQVFGGISMAASILTAGVGGVSSLLSKFGNVANKIGSSVVKVVEKAAEALV
                     KNVFAKISTVAEGVTNGIRSAGTTALNNEAAQLQMLSQLAAFAVQNLTRQSESLGESA
                     KLELDKAASELQNQASYLQSVSQLMSDSARVNSRIVSGRI"
     gene            1976..2914
                     /gene="espB"
                     /locus_tag="ACHJ48_004760"
     CDS             1976..2914
                     /gene="espB"
                     /locus_tag="ACHJ48_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO4710129.1"
                     /translation="MNTIDNTQVTMVNSASESTTGASSAVAASALSIDSSLLTDGKVD
                     ICKLMLEIQKLLGKMVTLLQDYQQKQLAQSYQIQQAVFESQNKAIEEKKAAATAALVG
                     GIISSALGILGSFAAMNNAAKGAGEIAEKASSASSKAAGAASEVANKALVKATESVAD
                     VAEEASSAMQKAMATTTKAASRASGVADDVAKASDFAEDLADAAEKTSRINKLLNSVD
                     KLTNTTAFVAVTSLAEGTKTLPTTISESVKSTHEVNEQRAKSLENFQQGNLELYKQDV
                     RRTQDDITTRLRDITSAVRDLLEVQNRMGQSGRLAG"
     gene            2921..3328
                     /gene="cesD2"
                     /locus_tag="ACHJ48_004761"
     CDS             2921..3328
                     /gene="cesD2"
                     /locus_tag="ACHJ48_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO4710130.1"
                     /translation="MVDTFNDEVFNYYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTHLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            3364..3585
                     /gene="escF"
                     /locus_tag="ACHJ48_004762"
     CDS             3364..3585
                     /gene="escF"
                     /locus_tag="ACHJ48_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO4710131.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            3591..>3832
                     /gene="escG"
                     /locus_tag="ACHJ48_004763"
     CDS             3591..>3832
                     /gene="escG"
                     /locus_tag="ACHJ48_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFO4710132.1"
                     /translation="MVNDISANKILVWAAVAAANHKLPKYAEAILNVFPQIIPDKKDI
                     AHLEFIILFGLNRKNDAVKALEDCMDDETSQLLYSLV"
BASE COUNT         1136 a          703 c          920 g         1073 t
ORIGIN      
        1 ctggttgctg gagagtcctt ctcgggtatc gattgtcgaa gataaacata tttattattt
       61 tcatttattg aaagactttt ttacatcatt accagatgct tgctttattg atagtgagca
      121 gagagagaat gcattattaa tgattggtaa agttatcgac tataaggagg aaattatttg
      181 atattaatta ctcaataatt tttttgtttt cctgagaaaa attatcaaga ggtatataga
      241 tggatacatc aaatgcaaca tccgttgtta atgtgagtgc gagttcttcg acatcgacga
      301 tctatgactt aggtaatatg tcgaaggatg aggtggttaa gctatttgag gaactcggtg
      361 tttttcaggc tgcgattctc atgttttctt atatgtatca ggcacaaagt aatctgtcga
      421 ttgcaaagtt tgctgatatg aatgaggcat ctaaagcgtc aaccacggca caaaagatgg
      481 ctaatcttgt ggatgccaaa attgctgatg ttcagagtag cactgataag aatgcgaaag
      541 ccaaacttcc tcaagacgtg attgactata taaacgatcc acgtaatgac ataagtgtaa
      601 ctggtattcg tgatcttagt ggtgatttaa gcgctggtga tctgcaaaca gtgaaggcgg
      661 ctatttcagc taaagcgaat aacctgacaa cggtagtgaa taatagccag ctcgaaattc
      721 agcaaatgtc gaatacatta aatctcttaa cgagtgcacg ttctgatgtg caatctctac
      781 aatatagaac tatttcagca atatcccttg gtaaataacc ggagataact atgcttaacg
      841 taaataacga taccctgtct gtaacgtctg gggttaatac cgcctcgggt acttctggta
      901 ttactcaatc tgaaacgggt ttatcgctgg atttacaact ggttaaatcc atgaactcgt
      961 cagcaggctg gacagaaagt agccctttac cgacgccgcc ggcaggtcac tcattagtga
     1021 cgccctctgc tgctgaggat gtccttagta aattgtttgg tggtattagt ggtgaggtta
     1081 caagtcgcac tgaggaggca gagccacagc gcacaagcta tccctatctc tctcaggtga
     1141 ataccgttga ccctcagcaa atgatgatga tggtcactct gttatccctg gatacttccg
     1201 cgcagaaagt ctcgagtctg aaaaactcta acgagattta tatggatggg caaactaaag
     1261 cgctggagaa taaaacgcag gagtataaaa aacagctcga agaacaacag aaagccgaag
     1321 agaaatcaca aaaaagtaaa attgttggcc aggtctttgg ttggttgggc gtcgcattaa
     1381 cagccgttgc cgctgttttt aacccagcac tctgggctgt tgttgccatt ggtgcaacag
     1441 caatggcact gcaaacggca gtcgatgtaa tgggggaaaa tgcccctcag ggattaaaga
     1501 ctgcagcaca ggtctttggc ggaatatcta tggccgcaag cattctgaca gccggcgttg
     1561 gcggggtgtc ttcactgtta tctaaatttg gtaatgttgc taacaaaatt ggctcaagcg
     1621 ttgtaaaagt cgttgagaag gcggcagaag cgctggttaa aaacgttttt gcaaaaattt
     1681 cgacagtggc tgagggcgtt acgaacggta ttcgttctgc cgggacaact gcgttgaata
     1741 atgaggctgc gcaactccaa atgttgtctc agttagctgc tttcgcggtg caaaacttaa
     1801 ctcgacagag tgaaagctta ggtgagagtg cgaagctcga gctggataaa gcggcaagcg
     1861 agttacaaaa tcaggcgagc tatttacaaa gtgtttctca actgatgtcc gattcagcac
     1921 gggtaaatag tcgtattgtt agtggccgaa tttaattatt aaagagaatt ttattatgaa
     1981 tactattgat aatactcaag taacgatggt taattccgct tcggagagta cgaccggcgc
     2041 ttccagtgca gttgccgcat ctgctttatc aattgattca tctctgctta ctgatggtaa
     2101 ggttgatatt tgtaagctga tgctggaaat tcaaaaactc ctcggcaaga tggtgactct
     2161 attgcaggat taccaacaaa aacaattggc gcaaagctat cagattcagc aggccgtttt
     2221 tgagagccag aataaagcta ttgaggaaaa aaaagccgcg gcaaccgctg ctttggttgg
     2281 cgggattatt tcatcagcat tggggatctt aggttctttt gcagcaatga acaacgcggc
     2341 taaaggggct ggtgagattg ctgaaaaagc aagctctgca tcttcaaagg ctgctggtgc
     2401 ggcttctgag gttgcaaata aagctctggt caaggctacg gaaagtgttg ctgatgtcgc
     2461 agaggaggca tccagtgcga tgcagaaagc tatggccaca acaacgaaag cagccagccg
     2521 tgcatctggc gttgcagatg atgttgcgaa agcctctgac tttgctgaag atcttgcaga
     2581 cgccgccgag aagacaagca gaatcaataa gttgttgaat tccgtagata aactgaccaa
     2641 taccacagca tttgttgccg tgaccagtct tgctgaaggt acgaaaacgt tgccaacaac
     2701 aatatctgag tccgtcaaat cgactcatga ggttaatgaa caacgtgcga agtcgctgga
     2761 aaacttccag caggggaatc tggagctgta taaacaagac gttcgcagaa cgcaggatga
     2821 tatcacgact cgtctgcgtg atataacgtc cgctgtccgc gatctccttg aggtccagaa
     2881 tcgtatgggg caatcgggtc gcttagctgg gtaattgatc atggtcgata cgtttaatga
     2941 tgaagtgttt aattactatc ttgaacaaaa agggtacaca atacagaagg agtttctttg
     3001 tggcagtgcc ttttttatcg gatggcggat tgagacacct tttttttcat tagcgtacag
     3061 actggatgaa caagaactga ttttgtgctc ttttgaagca cgtaaccaaa cagggcttaa
     3121 cggccctgtt ttatcgctga ctcatttgct cgaagagttg tatcaccatt tttcgggtat
     3181 taagaaaatc agcgcgatga aatctaagat tggttcagat tcagaacgtc aaaagcgcga
     3241 agagttgttt aattacttca tcagaaaggg tgccgtgcag caagaaacag aagacggaat
     3301 ttggttcgta atgaacgtaa atagttaaaa gtttttattt tattaacttc tgagggaaat
     3361 ttaatgaatt tatctgaaat tactcaacaa atgggtgaag taggtaaaac gctgagcgat
     3421 tctgtgccag agttacttaa tagcaccgat ttggttaatg accctgaaaa aatgttagag
     3481 ttgcagtttg cggttcagca atattctgct tatgttaacg tagaaagtgg aatgttgaaa
     3541 acgataaaag atctggtctc aaccatttct aaccgtagtt tttaatttta atggttaatg
     3601 atatttctgc taataagata ctggtgtggg cggctgtagc tgcggcaaac cataagcttc
     3661 ccaaatatgc ggaagctatc ctgaatgtat tcccacaaat tatacctgat aaaaaagata
     3721 tcgcacattt agaatttatt atcttatttg gattaaatag aaaaaatgat gcggtaaagg
     3781 ctttggagga ctgtatggat gatgagacga gtcagttatt gtacagtttg gt
//
LOCUS       DAZFDC010000243         3788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFDC010000243 DAZFDC010000000
VERSION     DAZFDC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.245"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..189
                     /locus_tag="ACHJ48_004764"
     CDS             <1..189
                     /locus_tag="ACHJ48_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4710133.1"
                     /translation="GVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGSLLFGLLTYL
                     TNLYFKIKEDRRKAARGE"
     gene            193..396
                     /locus_tag="ACHJ48_004765"
     CDS             193..396
                     /locus_tag="ACHJ48_004765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710134.1"
                     /translation="MNPEEMNRRISRLENEIAELNKKLSVQIPSEDEKKRRDEQFAAF
                     YDYCIEVTRRNFVKIFEESKSLQ"
     gene            716..1249
                     /locus_tag="ACHJ48_004766"
     CDS             716..1249
                     /locus_tag="ACHJ48_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4710135.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLNEKEGNHLTAYRD
                     GAGIWTICRGATMVDGKPVVPGMKLTKEKCAQVNAIERDRALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDQ"
     gene            1255..1767
                     /locus_tag="ACHJ48_004767"
     CDS             1255..1767
                     /locus_tag="ACHJ48_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO4710136.1"
                     /translation="MFANAWLKVATVSALLIASFVAGSMWTERAWEKKWADRNSEESS
                     QAVNAQIAARMIEQGRVIARDEAVKNAQEQAAKAAANVAGLSATVNQLRTEATKLTVR
                     LDAAKHTSGLAAAVGSKTTATNAAMLADMLGSITEEARYYAERADESYLAGMTCEHIY
                     DSVRDSSSIK"
     gene            1956..2378
                     /locus_tag="ACHJ48_004768"
     CDS             1956..2378
                     /locus_tag="ACHJ48_004768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710137.1"
                     /translation="MSEVAVELEEDSWPDFFPVGVPPKSAVPAEGEFFRLVRSNPPTP
                     ACFLSTHEEYPQRHKKYRGEALQCVYGTSFFSESRGATDAKAKFPAALGHRLVASGQV
                     TPVFGVMKKTFADPAHYTIWLKRNCAIHEHFHCIGEGV"
     gene            2375..3667
                     /locus_tag="ACHJ48_004769"
     CDS             2375..3667
                     /locus_tag="ACHJ48_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6575 domain-containing protein"
                     /protein_id="HFO4710138.1"
                     /translation="MSNVFLPNTMMGTLCYKRVYEFFEEPRFFSVENEVSTLFIVYWI
                     GEDDECDSWFIIPISPGRLELIERKRICIRDALINQEQNFFYEAHIPYDRTGKVTWLS
                     KNLVDIFEIPLPVSELYISSVVPVMPNGKLGEAITYSTHEIHLEKSSKKSAKNLVLSH
                     VSNVCDRFSDLYDRMLEMAKCKDKLRPVDARPGSFIISFKAEKLHSFEDTFKELSRLI
                     ELRADIIPFVFEKGIDVPALTDLLQSIVETGTNMELKSNQTGELAFVITKAGAEFYLK
                     KLSRLSTSLVGGHQIPQADVLETVYNLVEIVWSGEPLTPINTGLQDRHIYYYKHAAKV
                     LGLLDSFGNVTTSGQQLVQSDEMTRHKIAARKFEVSHVGWAWINWASVEHLSQIDPDS
                     ALDFLLEQCHSLSRDTIERRATTIKHWCRELKNYYTPL"
BASE COUNT         1059 a          746 c          937 g         1046 t
ORIGIN      
        1 ggtgtgtcat acaccacgtc agcggtggga acgggctact ggttcctgca gttgctggac
       61 agggtttccc cgtctcagtg ggcggcaata ggcgtgctgg ggagtctgct gtttgggctg
      121 ctgacatatc tgacgaacct gtatttcaag attaaagagg accggcgtaa ggcggcgcgg
      181 ggagagtaga cgatgaaccc tgaagaaatg aatcggcgca taagtcgcct ggaaaatgaa
      241 attgctgaac tgaataaaaa attgtcggtg cagattcctt ccgaagatga aaaaaaacgc
      301 cgcgatgagc agtttgcagc gttttacgat tattgtattg aagttactcg caggaatttt
      361 gtgaagattt ttgaggagag taaatctctt cagtaagctt aatggaggac tctgcaatta
      421 attcaggaag gtccgcaagg tcatctgtca gtgggaatga tgaaaaatcg gcggcagttc
      481 tgttaagaat tgttttaacg aattcctttt cattatccgg caacaagttg attagagcta
      541 cgacagcttg cctgagtgcg attaaatcag caaaagtttg ttttggtaga tttggataat
      601 ccatagtcac ctctgtgttt atcagattga cataccctgg ccagtgccca ccactggtgg
      661 gatgaagtct taacatatcc agggattcgg aaccgataaa tcctgataaa tatccatgaa
      721 cacaaaaatc aaatacggcc tgtcggctgc cgttctggcg ctgattgccg caggggcatc
      781 tgcgcctgaa atcctggacc agtttctgaa tgagaaagag ggcaatcacc tgacggcgta
      841 ccgtgatggt gcaggaatat ggaccatctg ccgtggtgcc acaatggtgg acggtaagcc
      901 agtcgtcccc ggcatgaaac tgacgaagga aaaatgcgct caggttaatg ccattgaacg
      961 cgacagggcg ctggcgtggg tggagaaaaa tatcagagtg ccactgaccg aaccccagaa
     1021 agcggggatc gcatcattct gtccgtacaa cattggcccc ggtaagtgtt tcccgtcgac
     1081 gttttataaa cgaattaatg ctggcgatcg caggggagcg tgtgaggcga ttcgctggtg
     1141 gattaaggac ggtggcagag actgccgtat ccgctcaaat aactgctacg gtcaggtatc
     1201 ccgtcgtgat caggagagtg cactggcgtg ctgggggata gaccagtgaa taacatgttt
     1261 gctaatgctt ggttgaaagt tgccacggtt tcggctctcc tcattgcctc atttgttgca
     1321 gggagtatgt ggacggaacg agcatgggaa aagaaatggg cggatcgtaa tagtgaagaa
     1381 tcctctcagg ctgtgaacgc tcagatagct gctcgtatga ttgaacaagg gcgagtgatc
     1441 gctcgtgatg aggctgttaa aaatgcacaa gaacaggctg caaaagcggc tgcgaatgtt
     1501 gctggcctgt ctgccactgt taaccagctg cgcaccgaag caacaaaact taccgtccgc
     1561 ttggacgctg caaagcacac ctcaggtctt gccgctgccg tcggaagcaa aacaaccgcc
     1621 accaacgccg cgatgcttgc cgacatgctc ggaagtatta cagaagaagc tagatactat
     1681 gctgagcgag ctgatgaaag ttacttggct ggaatgacat gtgaacacat ttacgactca
     1741 gtgagggact caagtagtat taaatagctg gtatgatgat gttgagaagg cggcacagca
     1801 ggagctgtga tcgacgagtg ctaatctagc acaattgagt tcacaaattg tctacttttg
     1861 ctatattgca accgatgcca acagcctttt ttgagcaaaa atcataaatg ctatcgtgtt
     1921 ggcagttttt ttctttgacg acacggaatg attttatgtc agaagtagct gttgaacttg
     1981 aagaggattc ttggcctgac tttttcccgg ttggagtacc gccgaaatca gccgtccctg
     2041 ctgagggtga gtttttccgt ttggtaaggt ctaatccacc aacccctgca tgtttcctct
     2101 ctacacatga agagtatccg caacggcaca aaaagtatcg tggggaagca cttcagtgtg
     2161 tctatggaac atcattcttt tcagaatctc gcggggctac ggatgcaaaa gctaaatttc
     2221 ctgcagctct tggtcatcga ctcgttgcta gtggtcaggt cacccccgtt ttcggagtaa
     2281 tgaaaaaaac atttgccgat ccagcgcact acacaatttg gcttaagcgt aattgtgcta
     2341 ttcatgaaca ttttcactgt ataggagaag gcgtatgagt aacgtttttc tgcctaatac
     2401 aatgatgggc actctatgtt acaaaagagt ttatgagttc ttcgaagagc ctcgcttctt
     2461 ttcagttgaa aatgaagtta gtacactttt tatcgtttac tggattggtg aggatgatga
     2521 atgtgatagt tggtttatca taccaatttc tccagggcga ttagagttga ttgaaagaaa
     2581 aagaatatgt atccgtgatg ctttgattaa tcaggagcaa aactttttct atgaagcgca
     2641 tataccatac gatcgtacag gcaaagtgac ttggctgagt aaaaacttgg ttgatatatt
     2701 tgaaatacct ttgcctgttt ctgaactata tataagttcg gtagttccag taatgccaaa
     2761 tggtaaactg ggcgaggcta ttacttactc aactcatgag atccatttag aaaaaagctc
     2821 aaagaaaagt gcaaaaaatt tggttttaag tcatgtgtca aatgtatgcg acagattcag
     2881 cgatctttat gacagaatgc tggagatggc aaagtgcaaa gataaattac gtcctgtcga
     2941 tgcgcggcct ggttccttta tcatttcttt taaagctgag aaattacatt cctttgaaga
     3001 cacttttaaa gaattaagca gactgataga acttagagct gatattattc cttttgtttt
     3061 cgaaaagggt attgatgttc cagctttgac agatctctta caatccatag ttgaaaccgg
     3121 aacgaatatg gaactcaaaa gcaaccagac aggtgaatta gcttttgtta ttacaaaagc
     3181 tggtgctgag ttctatttaa aaaaattatc acgtctttct acctcattgg ttggagggca
     3241 tcaaatacct caagcagatg tcctcgagac agtttataat ttagtggaaa ttgtttggag
     3301 tggcgaacca cttactccta ttaatactgg tttgcaagat agacatatct actattataa
     3361 gcatgctgct aaagttttgg gattacttga tagctttggt aatgtcacaa cgtctggtca
     3421 acaactcgta caaagcgatg agatgactag acataaaatt gcagcgagaa aatttgaagt
     3481 gagtcatgtg ggatgggctt ggataaattg ggccagtgtt gaacatttat cccaaattga
     3541 tcctgacagc gctttagact tcctattgga gcaatgccac tctttaagtc gtgatactat
     3601 tgaacgacgt gctacaacta ttaaacactg gtgccgtgaa ttgaaaaatt attatacacc
     3661 tctgtaaagg ataattttcg tagttaacaa tgaaccactg acgtctgtcg gtggtttttt
     3721 aatagataat ttcccactta tccacttgat ttttgatggg tcctcctggt gggggggcct
     3781 gtccacgg
//
LOCUS       DAZFDC010000244         3755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFDC010000244 DAZFDC010000000
VERSION     DAZFDC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.246"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..121)
                     /locus_tag="ACHJ48_004770"
                     /pseudo
     CDS             complement(1..121)
                     /locus_tag="ACHJ48_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(121..537)
                     /locus_tag="ACHJ48_004771"
                     /pseudo
     CDS             complement(121..537)
                     /locus_tag="ACHJ48_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(537..722)
                     /locus_tag="ACHJ48_004772"
     CDS             complement(537..722)
                     /locus_tag="ACHJ48_004772"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710139.1"
                     /translation="MDVRNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVPPPF"
     gene            899..3109
                     /gene="katP"
                     /locus_tag="ACHJ48_004773"
     CDS             899..3109
                     /gene="katP"
                     /locus_tag="ACHJ48_004773"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO4710140.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            3153..3542
                     /locus_tag="ACHJ48_004774"
     CDS             3153..3542
                     /locus_tag="ACHJ48_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO4710141.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            3599..3755
                     /locus_tag="ACHJ48_004775"
                     /pseudo
     CDS             3599..3755
                     /locus_tag="ACHJ48_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3655..>3755
                     /locus_tag="ACHJ48_004775"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1054 a          800 c          966 g          935 t
ORIGIN      
        1 cctcctgtgt ggcaacatac gtcaggtccg ccacccagac ctgatttggt gctgcaggag
       61 cgaacgtctg gttcagcaga tttggcgcaa ctggcagatt gtggttcgag ttcgtagtcg
      121 cacatctgcc tggcggcaga tttcctgtat cacatccgca gcaatgcggc tcatctctgc
      181 cagtaaccac cggttgtgga acaccaggga ccagtactgg cagggaagtg tgaacacaat
      241 atgctgccac ggacagtcgg gaaccagact cagcagatac tgtatccact gtgcgccagc
      301 cttcactccg cagtgcgggc aggagcgact tttacaccgg aagcagacct tttttatgtg
      361 gctgcagtcc ggagatgaac agcaccactg tgtgtacccc atcagcgtgg tcccgcaggc
      421 catgattttt gtcacagact caatgactac cggacgcact gaaccttccg gttgtttctc
      481 cagccagtta agccagcggt ttccctgctg aaaaatgtcg gcaaaacggg gaagcatcag
      541 aagggcgggg gaactccgtc cggccagtga accgtgccac actccgggca gtacatgccg
      601 ccggcgctga taccggcaag aatggtcgca aactcccgct ccgtgcagcg ggctatttca
      661 ggataccctt cgtcatcaac acgtacaaac cagaagacca gctttttgtt tctgacatcc
      721 acaaagaagg gaatattcag gtctgcgcag cactcaacgg catcgtcagt tgcggcttgg
      781 aaccccttag tattttttgt ctgtagtatc tatcccagca ataggtatat cctgttgcat
      841 caataaagtt gacttttgta tacaacatgc gaatttccct taatccggag ctattcgtat
      901 gataaaaaaa actcttcctg ttctgattct tctggcgcta tcggggagct tttctaccgc
      961 tgtagccgct gataaaaaag agactcaaaa tttctactat ccagaaacac tggatttaac
     1021 tcctctgaga ttacacagcc ctgaatcaaa tccctggggg gctgattttg attatgccac
     1081 cagatttcaa cagctggata tggaggctct gaaaaaagat atcaaagatt tgctgacaac
     1141 ttcccaggat tggtggcctg cggattatgg tcattatggt cctttcttta ttcgtatggc
     1201 ttggcacggt gccggaacat acaggacata tgatggccgg ggaggcgcca gtggtggtca
     1261 gcaacgtttt gaaccgctga acagctggcc ggataacgtt aatctggata aagcccgtcg
     1321 attgctgtgg ccagtcaaga aaaaatacgg ctccagtatt tcctggggag acctgatggt
     1381 cctgactggt aatgttgccc ttgaatccat gggatttaaa acgctgggat ttgctggcgg
     1441 aagagaagat gactgggagt cggacctggt atactggggg cctgacaaca agcctcttgc
     1501 agataaccgg gataaaaacg ggaaacttca gaaacctctt gccgccacgc agatgggact
     1561 tatttatgtc aatcctgaag gccccggtgg aaaaccagat cctctggctt ccgcgaaaga
     1621 tatcagggaa gctttttcac gtatggccat ggatgatgag gagactgtgg ccctgatcgc
     1681 gggagggcat acatttggta aagcacatgg tgcagcgtct cctgaaaaat gtattggcgc
     1741 agggcctgat ggtgcacctg tggaggagca gggactggga tggaaaaata aatgtggtac
     1801 aggaaacggc aaatatacca tcaccagtgg cctggaagga gcctggtcga catcgccaac
     1861 ccagttcaca atgcagtatc tgaagaattt atataaatat gaatgggagc tgcacaagag
     1921 tcctgccggt gcttatcagt ggaagcctaa aaaagcggca aatatagttc aggacgcgca
     1981 tgatccgtct gtcctgcatc cgttgatgat gtttacgacg gatattgctc ttaaagttga
     2041 tcctgaatat aagaaaataa ccacccgttt cctgaatgat ccaaaagctt ttgagcaggc
     2101 attcgcaaga gcatggttta aactgaccca ccgggatatg ggaccggcag cccgatatct
     2161 tggtaatgaa gttcctgcag aatcatttat ctggcaggat cctcttcctg cggcggatta
     2221 tacaatgatt gatggtaaag acattaagtc gctgaaagag caggttatgg atttgggtat
     2281 ccctgcatct gagctgataa agacagcctg ggcttcagct tccacatttc gtgtgactga
     2341 ttatcgtggg ggaaataatg gtgcccgcat caggttacag cccgaaatta actgggaagt
     2401 taatgagcct gaaaaactga agaaagtact ggcatccctg acctcattac agcgtgaatt
     2461 taacaaaaaa cagtctgacg gaaagaaagt gtcgttggct gatttaattg ttctttcggg
     2521 taatgctgca atcgaagatg cggccagaaa agccggggtg gaacttgaga ttccctttac
     2581 tccgggaaga actgacgcct ctcaggagca gacggatgtt gcctcattca gtgtactgga
     2641 gccgacagca gatggattca gaaattatta ctcaaaaagc agaagtcata tatcgccggt
     2701 tgaaagcctc attgataaag ccagtcagct ggatctcacc gttcctgaaa tgacggcatt
     2761 actgggtggt ctgcgggtaa tggatattaa tacaaataat tcttcgttgg gagtgtttac
     2821 cgatacccct ggtgttctgg ataacaagtt ttttgttaat ctgctggata tgtcaacacg
     2881 atggagtaaa gcagataaag aagatacata caatggattc gatcgtaaaa cgggagcatt
     2941 aaaatggaaa gcatcctctg ttgatttaat cttcagttca aatcctgaat tacgtgcggt
     3001 ggcagaagta tatgcctcgg atgatgcgag aaataagttt attcatgatt ttgttaaatc
     3061 gtggaataaa gttatgaata gcgatcggtt tgatttaaac aataaatgat aaaactgatg
     3121 agggagggca cctccctcat gaaggagaaa ttatgcagaa aagcaataaa gttataatca
     3181 gctgtggtat ggcattatat tttttgtgtg gggggattac agtggcagaa gaacttgatg
     3241 aaagcatgca agttcttgca aggaatatga gtgtacttca gtcatcaatg gataaaaagg
     3301 agcttcttga agctcttgac agaatggatg atgctgtaga tgagtcaatg aagcgtcttc
     3361 ctgagagcat ttcaccggct gatgaagaag ggaagaatga ctatataaaa gaacttcaaa
     3421 agcttgaaaa agagattatg ctggcgaaac aaaaagtctt atcgggacag ttatcagata
     3481 tacccgattc ggtaaacaag atgaataaaa tcagagttga ggggcatgat aaattcagat
     3541 aaatcagttg gttgattcct gcgggacgaa tacatacgtc aggaacataa ctttatggct
     3601 ggaacgtgaa aatcgtgaac tgcgccgcag taacgatatc cttcgccagg cttccgctta
     3661 ttttgcgaag gcggagttcg accgcctctg gaaaaaataa tgccactgct ggataagctg
     3721 cgtgagcagt acggggtcgg accggtatgc agtga
//
LOCUS       DAZFDC010000245         3728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFDC010000245 DAZFDC010000000
VERSION     DAZFDC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.247"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..630)
                     /locus_tag="ACHJ48_004776"
     CDS             complement(<1..630)
                     /locus_tag="ACHJ48_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO4710142.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     VTDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAVAEKCGYSS"
     gene            complement(768..964)
                     /locus_tag="ACHJ48_004777"
                     /pseudo
     CDS             complement(768..964)
                     /locus_tag="ACHJ48_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
     gene            complement(1251..2069)
                     /locus_tag="ACHJ48_004778"
     CDS             complement(1251..2069)
                     /locus_tag="ACHJ48_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO4710143.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALFFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(2221..2592)
                     /locus_tag="ACHJ48_004779"
     CDS             complement(2221..2592)
                     /locus_tag="ACHJ48_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4710144.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     KCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(2582..2953)
                     /locus_tag="ACHJ48_004780"
     CDS             complement(2582..2953)
                     /locus_tag="ACHJ48_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4710145.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(2966..>3728)
                     /locus_tag="ACHJ48_004781"
     CDS             complement(2966..>3728)
                     /locus_tag="ACHJ48_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4710146.1"
                     /translation="HGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQN
                     LAAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSV
                     YRESDIIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDPESPKSFMKR
                     PKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHH
                     NELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1143 a          946 c          703 g          936 t
ORIGIN      
        1 gcttgaataa ccacattttt ctgctacagc ctgtccatat ccataccggg aacataacat
       61 atttacagca acacgcatcc gctcttccag caacaagcga ctgaacatgc ccccttcatt
      121 tttcagtttt gtctttaaca aactctcact catatgcaac tgtagagcaa tcgcaccaag
      181 cgtccagctt gctgatatat ctgtctgaat tatcgccctg actttggcac ttatgctgga
      241 taaacatcca cttaaaaaca atgacatccg ttcatctgtt tcaaacagag acaggcaggc
      301 catcataaga aacatatccg tgacctctcc ggaaaatccc tggctggtaa ttaaagccgc
      361 agccaacgca ggattgttgg gttccagcaa caggtaaagc ggaatgtcag tcagacgagt
      421 cctcgtcagc ttatgctgac tttccagata ttgacttacg acggattcgc ttatatcgac
      481 aattttaact ttgccataat gcataaggaa aagctccctg atgcatttgg tggccagaac
      541 gactgagcct ggcttaagtg acaacgtatc cttttcaaga aaaatattaa ttggggagca
      601 aaccatgata actgaacaga caacaaccat tataatttta ctttcattag caattggtta
      661 gttcaattat agccccaaaa ggtaaattat catcaacaca taagcaaagg actgacaggt
      721 gccgctaaca cccaccagcc gcccatttac cacaaataaa aaagccttca ggactgaagg
      781 cgtctgtaac aaccgaactg atagtctgcc agacccgcat aaccagctgg gtcagtatta
      841 actggcagcg ctcgcgtgaa aggtacgtat tctgcgcaat ctccccgacc gtcgccggtt
      901 cggtgacgct taattcatca aacacaactc tggcggtttc tgtcatatcc tgctgtttca
      961 gcatgtcttt ttaccctttc cggttaacgt gacacaccaa taactcttgt cgaaaaagcc
     1021 agcaagctga aagacctgta ttaataacta ccagcacatt taacgcactg cgctactttg
     1081 cgggcacaaa aaacccgctc agaggcgggg tcaagctatg cggcgaaata accactctta
     1141 acagcatacc tgatttttta cgtacgtaaa tgctttgccg tgcatatttt tcatgcaaat
     1201 gtcacgtcct gctatttttc agtcttataa atttaaaacc atagaaaaaa tcaattatgt
     1261 tttaaaaatg gataggtaaa gaataacaag tgacacagat tcaaaccaaa atggaaaagg
     1321 gtggcaaccc acaaacgccc actccacatc catgacaatc catacacaac accagataac
     1381 gtggcaaata atacaagtaa agcaccacct gaatagtgat aaaaaccaaa caacaaagcc
     1441 gccacaatta atgcaaccaa tggagacgtt acttctgata gccgtgattg aatataccct
     1501 ctaaataatg attcctctgc cagagacaca aaaaacaaat tagccaatat aaactctggc
     1561 aaccactcag gaaaatgaat ctctggcttt aatccaccaa aaaaaacagc caaaaacagg
     1621 ataagaggaa cagagagcga cagagccccc cacttccaca aagacacttc tgattttact
     1681 tcttttttaa acaaagaaga tgtacacagg actaacaaaa atggcaccag cgctttatca
     1741 aaattaaaat acattgtata gggagtactt tgagggccaa cagtaacaga atttagcact
     1801 acaggattgt gaaaccctgg ccatagatgg aaaaataatg ctatggctga taaaactatg
     1861 ccaacttcat atatagattt agcccaggcg ttatatttcc agttgaactt caaaataata
     1921 aaaaaaacga ttgtagcaac aaaaaataac accgaccaat caataatatc attaagcaca
     1981 gccaggacaa cagacaccgt caacaatgaa aaagccacta ccttatgcca actgagagtt
     2041 gacagtgaca gtactaacac aatccacata agcactcctt ttatttaatg atgaagattg
     2101 attatcaata ttttcaattc accaggcaac attttatcta ccttccacaa tacatgacca
     2161 tcaaaaaata aacatgttaa ttataaacac agaaaacata accctcatca ctatatatcc
     2221 ctaccgcata tccatatcta accggacatt cagagccata agcattcctt caataatacc
     2281 ttccgcttta taaagccttt taccaataag cccatcagaa catctatgct tacgtgcaag
     2341 agccataaat gtcattccac ctacgtaata atccaccaac aaatcgtgca aatactgatt
     2401 attcttgttt aatcgtgcca tacaaccaca aattatcatg gcgtcatcat cagaacactt
     2461 aggacgtgat ttcactttag tcgggattaa tcctttaaaa ccagcagcta tcgagggcca
     2521 tgttacatct tcatgattat ttgctgccca tgccccccat cgctcaagaa ccatctggat
     2581 atcacgcgcc atcgttacca cctctgattt cgtaaatctt cacgcccagc cgtccacctg
     2641 gcacaggctg accgcgcaca atattgattt catcaaactg ctcgtcatca atgagcactt
     2701 ccgcatgcgt cagcgcatcc agcggtgctt tcagaatgtt gtccaggtca cggcggcgct
     2761 tatccggtgg ttctgcaata atttttattg ccagccgtcc ggacaggctt aatttcagcc
     2821 gctgctggcg aacaataagc gccacagccc ggcgataacg ctcaccggct tttgatacaa
     2881 aatatgtgct gccacgacgt cgccagtagg tgttcaccgt cggcgggtaa ggcaaaacaa
     2941 actctatacg catcagtaac ctcttttacc cgagcacgcc ggttgcaaaa gcgtgatcaa
     3001 gaaaacgaaa aattaaatca acctgggaac catgcttttc ttcgaacgcc agcggatccg
     3061 catgaagctc gttgtgatgc tcccgacaca gcggtagcgt gaaaatatcg tgggattttg
     3121 ttcccattcc accctgacca tgaccaatca gatgatgagg atcgtccgct ggcttaccac
     3181 aacacgcaca cggctgtgtc tttacccagc gtgtgtattt ctcatttacc cagcggcgac
     3241 gtttaggtcg tttcatgaag gatttcggag actccggatc aacggcaatg ctgaccaccg
     3301 tcttttcctg tggtgggttc tgttgctggt gggtgtgagg cggtagcgca atattttttg
     3361 tgcgctgctt cagtatgctg gtggcggtct gttctcccgg tatgatgtcg ctttcacggt
     3421 atacggagcg gattttttcc gccggtaatc ccagagaacg acgcgatact gcctcaggta
     3481 atgcgtccac cacctgattg cagactgccc accaggataa ttcagccagc gataattccc
     3541 gttcctgtat gccattcatt gcgtggcgga tgacgtcaat catccaggcg gccagattct
     3601 gttgagcaag ttgctcaagt gattcggatg tctggtcgcg cagctggttg tcgcagtgcc
     3661 agcacaacac catcgcgccg gtaccgtaac gatgtatgac agtttcgctg tgatgataat
     3721 caccatga
//
LOCUS       DAZFDC010000246         3724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFDC010000246 DAZFDC010000000
VERSION     DAZFDC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.248"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..901)
                     /locus_tag="ACHJ48_004782"
     CDS             complement(<1..901)
                     /locus_tag="ACHJ48_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO4710147.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQHTASLSSPDTPRPL
                     SDSAFPDTLPGTEYGPDRGIRLSA"
     gene            complement(968..3076)
                     /locus_tag="ACHJ48_004783"
     CDS             complement(968..3076)
                     /locus_tag="ACHJ48_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO4710148.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSIDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKNYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          808 a         1101 c          947 g          868 t
ORIGIN      
        1 ccgccgaaag gcggatacct ctgtcgggac cgtattcggt accgggcagt gtgtcgggga
       61 acgctgagtc tgaaagaggg cggggggtgt caggggaaga taaagaagcg gtgtgctgtt
      121 tacgggcctc ttccgcccgc tgcgcctgcg tggtcagcac cagacggaac tcccgcccgg
      181 caccgtccgt cacgccggtg atttccccgg ccaggtcacc ggcggcctca cgcctgtacg
      241 tcagcgtccg cccgaagcgg tccgccatcc cggtaagcac ccggtacggc ggcagcggcg
      301 ctggcagtac gtcctcagca cccggcaccc gctctgacca ccccagtatc caccacggcc
      361 cctgtgcgct gttggtcgcc aggtaaagat gcgggcttaa ccggatatcc ggcggcagcg
      421 ccccccacag ccgcgccagc gtgtggccgt ccggctgcgc tgccttacca ccgcgcacca
      481 gccacattga ctcgctgcgg ctgtacaccg cctcccccgg cagcagcggc tcaaagtgaa
      541 tgctccgccc gccgttgtca ttgagtacca gtgcatcatc gcgtagctgt aagcggatat
      601 cagaaggcgc tttccagccg gggccaaaaa tccccaccgg cgcaggcgtc cgggtccggt
      661 agctgctgta ggtgcgggag agaatgaacg gcagcgggcc gggcagcgca aggtccgtct
      721 cgccgggcag caccttcgcc cccagcagcg gatttaccgg gttgcccgaa gtcatcccgc
      781 ccgggcacac cgagcacgcc acgccagttg gcgcgccaat tcttacacct gccgaaccct
      841 ggacaatggg accgccatac tgagtcatat ctccctgacg cgccgctggt tttccgctca
      901 tgattcaatt ccttcttctg tttattttta atgcttgttt gttactgaat accgaacagg
      961 tacgggatca gtactctctc atatccggcg tatcctgctt ttcatcaggg gtcttcggtg
     1021 gggatttcga ggctgcacag ttccagttaa tcaacagtga gtcaccatta acctgctcct
     1081 taccctgcaa atgaattttt gtgccgttga gaaaaatttg tccgtccttc gtcagcacca
     1141 gccttgcctt accacagcag agctccagat gctcaccggc ggagtaaatc gcggtctgcc
     1201 cggtatcatc ctttttcgtt ttaccaacgg tgaaggtaac gttatttccg actttgacgt
     1261 cataacccag cccgaccctc aacgatttat gcaaaccgat ctgcgaggac tgcatcagag
     1321 ccaccgaagt gttcataatg ccacctaccg tcgtctggta cgccacgccc acggtcagcg
     1381 cacgaacaag tccaatcgtt tccacctgaa cagaacccac gttaatgatc tggttactgc
     1441 ccaccgtctc tatctggtta acgcccaccg tctgtatctg attgttggta accgcaatca
     1501 tctgattgtt gccaatggtc tcagcatggt tgtttatcac atccgtggtg cggttattca
     1561 gcacctcggt gttcatgttc ttctgcgcgt ggatgtagac ctgttctttc cctgtcgcat
     1621 cgtcaaactt cagttcatta aacccgctgc ccttatagtt tttcgaacga atggtcatct
     1681 gcgtcttcgt ccccggcagg ctgccgggtg tgcggttttc ctggtggtag gtacgcccca
     1741 taataatcgg ctggtccgga tcgccgttga ggaagtccac aatcacctcc tgacccacac
     1801 gcggtatcgc caggttgcca aagccggtgc ctgcccacgc ctgtgccaca cggatccagc
     1861 atgaactgtc ctggtttgac gggttatagc ggtcccagtt aaatttcacc cgcacgcgac
     1921 catgttcgtc gcagaagatt tcctcgcctg ccggtcccgt cacgacggcg ctctgcgggc
     1981 catccaccag cggtttcagc aacggctgtg gtcgccatgt ccggtctgcc ggtattaccg
     2041 caaagtggtt attcagcgtg gtacctgaac ccctgcgtcc cggcaccgcc tgtggctgct
     2101 cgccgtgcag ttcacttgcc accacctgcc attcccggtt caggttcgcc tgcggatgcc
     2161 ccgtcagcac aattcgccgt cccggccata tttccggaga acggcttgtt ccgcgcgcca
     2221 cttctgcgtt gttgcgccag ccatccatct gccagcgggc aaagttctgc ccgtgggcac
     2281 ccttgaaacg tccggggtag tcatacactt catactgtgt gcgctggtaa tcctggtact
     2341 ggccttcctg atcaaaacgc cctgcccagc cgggtcgttt aaaggtgtag tccttggtca
     2401 ccacggaaga agggcggatt tgtgcgctgt agcggaactg gctgatgcat agggtgctca
     2461 cctcggtacg ggtgttcggg ttccagggga tctcaaagga ctccggcaga taacgcacgg
     2521 tgtcgcacag gaccaggctc tggtcgatac ttttttgcgc gtgctcctca taaaagaaga
     2581 tgccttcctc cgccgccatc cggcacagga aatcgtaatc agtttcgccg tactggacac
     2641 aaaactcacg ggaaggatgc ggctcgctga acagcggact ccactcggtc acaccgtttt
     2701 ctttcaggat cgtagcgagt atgctttcga tgtcctcatt ctggaagata cggaagttct
     2761 ggcgcagccc tgtgcgccac agcggcgggc acaccttcat gctgtacagc atctggtttt
     2821 tgtcgttctc ccccagttca aaccaggtca ccacaccttt cacccggcgc tgtacgtcat
     2881 cgccctgcca taccgtcagg taggccattt tgtccagcac ctgcgcaaat tcaagggaaa
     2941 gaaactgctg actgaccaga gaaaggtcga gggaaaaaag tgaagagagt gactggttca
     3001 gatgaaagga gaccaccgca aacgcgtccg gtggcaggcc gtccacttcc agcgtgaaac
     3061 gtaatccggt tgacatagca acctccgtga aaatgaataa taatccgcac atattgcatg
     3121 tacaacatgt cgggcggatg aaataagtga gtcgacatct ggcgttaccg gggaatatca
     3181 gaaaaatgtc atggttatgt gatgatttag tctgggattt tacttcagag aaacgggtgt
     3241 ctgtttagtc aacaaaataa tcacaaacgc aacggatggg ttctcgacgt tattcacgac
     3301 aggctcgtac attacagcga gtaataaaaa acctgatatg caaagtcaaa ccaatattca
     3361 taatgtcttt ttattcacaa caataaataa tttaataaca atcattgaaa tgaatgatga
     3421 tttgtataag gaatatacgc taccgcccca ccctgataac atgatctgca cattgccatc
     3481 ttaacgtcgt taaacactgg cttaatagtg gatatttcaa attaggaatt tattaaattc
     3541 acctgatgaa tatcataagc aaatagcata gaaaataatg acaactcccc cgaggggacc
     3601 atttccaaag cactgcacca ccacctggtg cccggatgat cttgcttcag tggtaagagt
     3661 acctgattca ttgttcccaa aatggtgttt tcccctgccc ctccaataat gtgcagctct
     3721 cacg
//
LOCUS       DAZFDC010000247         3583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFDC010000247 DAZFDC010000000
VERSION     DAZFDC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.249"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..183
                     /locus_tag="ACHJ48_004784"
     CDS             <1..183
                     /locus_tag="ACHJ48_004784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710149.1"
                     /translation="WRLAVVRCGWQQTITVTTRSPTKRSAIKKPESWNRRMPPLLTCR
                     CASVMLLRSMQNTRGN"
     gene            187..393
                     /locus_tag="ACHJ48_004785"
     CDS             187..393
                     /locus_tag="ACHJ48_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO4710150.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            458..682
                     /locus_tag="ACHJ48_004786"
     CDS             458..682
                     /locus_tag="ACHJ48_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710151.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1039..1179
                     /locus_tag="ACHJ48_004787"
     CDS             1039..1179
                     /locus_tag="ACHJ48_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710152.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1309..1494)
                     /locus_tag="ACHJ48_004788"
     CDS             complement(1309..1494)
                     /locus_tag="ACHJ48_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4710153.1"
                     /translation="MSSKNRPRRTTIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            1536..1901
                     /locus_tag="ACHJ48_004789"
     CDS             1536..1901
                     /locus_tag="ACHJ48_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4710154.1"
                     /translation="MAVLRTLPGRIKTLNTRRINVLRGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
     gene            2191..2754
                     /locus_tag="ACHJ48_004790"
     CDS             2191..2754
                     /locus_tag="ACHJ48_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4710155.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            2751..>3583
                     /locus_tag="ACHJ48_004791"
     CDS             2751..>3583
                     /locus_tag="ACHJ48_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4710156.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     V"
BASE COUNT          847 a          853 c         1014 g          869 t
ORIGIN      
        1 tggcggttgg ctgtggtgcg ctgtggctgg caacaaacca ttaccgtgac aacgcgctca
       61 cctacaaagc gcagcgcgat aaaaaagcca gagagctgga acaggcgaat gccaccatta
      121 ctgacatgca ggtgcgccag cgtgatgttg ctgcgctcga tgcaaaatac tcgagggaat
      181 tagccgatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgct ggtcgtaagc
      241 gcctgcggat caacgccacc tgccccggta ccgtgcgtga agccaccggc acctccggcg
      301 tgggcaatga tgccgccgtc gaactctctc cggttgctgg acgaaacgtt ctcggtatca
      361 gagacggaat catcagcgac caggcagcat tgagaatgct tcaggaatat atccgcactc
      421 agtgtattaa ctagtatttt tgttattcgg agaatgcatg aagaaattat tggtaaccgt
      481 aaagcctttt cagggaacaa ttccgttccg tattttgcag cgtggtcgtg ttcttgttga
      541 aggttcgttc agtggtaaat gtacgcaatt acactccagg acctttcagg tgaatgccac
      601 gaatgaagag ctaaccgttg agtgtacgat gaatgccgct aaatgccgca tggtatccgc
      661 tgcattacag ccagtgtgtt gagcgacctt attattcatg cgcggtattg tcgccgtatt
      721 cctgcattaa cagagaccgc agcccgaccg ggagaatcct ctgcgcgagt gtgcggggat
      781 aatcaaaaac gatacacacc ggggtttacc gcgttaacgg agcgcggcgt tgtcccctca
      841 tggtcgctgg tccggtgcga tggtggaaga agccggatgt ttatcactat taattgataa
      901 cacagaaatg gattcattga ttttcagcac gtttttgtat tcgtattatt gaacatctgt
      961 ttattttact tttaacatat tgataataaa aagagctgta aatctttaga tgagtcgatt
     1021 ttgtccgggg aagttcaaat ggattttatg ctgacggttt ctggtgtggt tatcctgtcc
     1081 attgcttata ctgcagataa atatggctgc catttgttat cacgtattgg cgcttattgc
     1141 tcgttgatgc tgattttctc gtcgcttttt tttgagtaag ttatattaat tataacaaat
     1201 aattttctgt gttttttcag gctatcccgt cagacgggaa gcctgtactg ccgggggacg
     1261 aatggaaaac tgatgtgtcc ggtaactgtg tgttctgtga acaccatgtt acttaattat
     1321 gtaattcata cccgaacgct ctgttgacag ccttcttctg caggcttcaa taacccacgc
     1381 tgaaaagtta ccggaacctt tatgttcaag ggcgatattg atctgttcaa tcatgtgatt
     1441 ggggaaacgg atattgcgga ttgtggttct gcggggccgg tttttcgatg acattttctt
     1501 tcctctggtg acaagctata tggcgaggat tttacatggc tgtgcttcgt acgttaccgg
     1561 gcagaatcaa aactctgaac acccggcgga taaatgtcct gaggggggaa cagcgtcgtg
     1621 tcagtggcag tgcccgggtt tccctcaaac gtcgtatctg gcggagggat gccggacact
     1681 gttgtctctg tagacgcgtg gttgaccttt gtgacagtga actcgatcac cgcattgcac
     1741 ttcagttcgg tggtggtaat gaggagacga acctctggac cctctgtact gaatgccatc
     1801 gccagaagtc agcgagtgaa gcggcgagtg gtatgcctga tccgacgttg cctgagcttc
     1861 ctgatggcac gctcagggcc gacggaatca ctggcctgtg accagacccg ggggggatca
     1921 tccggcgaaa aaaaacgatc gccctggaca ccgcgccccc tctcacgcag agaaaaaatt
     1981 cccgtttcag ggcagttaac atgttaactg gctgcccggg catttttgcg gtttttatct
     2041 ttattattca gtttgttgtg cggaaaaaat gttaacaggc tttttcagca aatgttaacc
     2101 aggcagcagt taacatttgc ggcatgagac gccgggaaaa atgggctgaa ccatacccgg
     2161 ctgagtgcgt tctggacccg ggaggaggct gtgctgacaa cgcaaaaacg aaaatttgcg
     2221 ctggcgctca tgtccgggaa aaacaaaaca gcgtcagccc ttgccgccgg ttattcggcg
     2281 aagacagcca gggttaaagg ctcgcagctg gcaaaagatc cggaggtgct tgcgtttata
     2341 gcccgtaaac agtgcgagac ggtggaggtg gatgaggttc ctgtttaccg gcagaaaaaa
     2401 tcagagccgg aggataaacc ccgtcgccgt gaggcggctg caataccaca gccggacgaa
     2461 acaaatccgg agatgccacc gcccgtggtg atatctcctg gtattgagta tatggaggac
     2521 ggtcttcccg atccggtgaa agcgatgggg cgtcttctgg tggagaacat taataccgac
     2581 cccaggctgg cgctggatgc ggcttataag ctggcgcagt tcacgcacca caaaaaaggg
     2641 gatgccggta aaaaatcggc aaaaggtgac gcggcgaaaa aagcggctaa ccgttttgcg
     2701 gtgccaccac caccccgcct ggtggtgaat aatgataatg agggcaacgg atgatacctg
     2761 tgtggagcac ggcctgcccg gactgggcag agcgcctgaa aaaggggctg tcgattattc
     2821 cggctccgat ttatccggag caggccgcac atgccctggc gatttttaaa caactgcgga
     2881 ttgtggatgc accgggcagc ccgacgttcg gggagtcctg cgcacagtgg gtgtttgacc
     2941 tggtggcggc cctgtttggc tcctacgatg cgcagaccgg tgtacgccat atcaaggaag
     3001 tttttatcct tatccccaag aaaaacagca agtccacgct ggctgccggg atcatgatga
     3061 cggcgctgtt actgaactgg cggcaggcgg cgggctacac cattctggcc ccgaccgtgg
     3121 aggtggcggc taacgccttc aaccctgcca gggatatggt acgacgggac gatgatctgg
     3181 atgacctctg tcaggtgcag acacatatcc ggaccatcac ccacagggtg acggacacca
     3241 ccctgaaggt ggtggctgcc gatccgaata cggtatccgg tatcaagtcc gtggggacgc
     3301 tgattgatga actgtggtta tttggcaagc agtacaaagc ggaggacatg ttacgtgaag
     3361 ccataggcgg ccttgcctcc cgcccggaag ggtttgtggt gtatacgacc acccagtcga
     3421 atgagccgcc agccggggtg ttcagacaga aactgcagta cgcccgggat gtccgtgacg
     3481 gcaaaattca tgatccgcac tttctgccgg tgatttttga gcatcctcct gaaatggtgg
     3541 aaagcggggc tcacctgctg atggaaaacc tcgccatggt taa
//
LOCUS       DAZFDC010000248         3576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFDC010000248 DAZFDC010000000
VERSION     DAZFDC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.250"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            55..1287
                     /locus_tag="ACHJ48_004792"
     CDS             55..1287
                     /locus_tag="ACHJ48_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO4710157.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKVR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRPLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            1272..1910
                     /locus_tag="ACHJ48_004793"
     CDS             1272..1910
                     /locus_tag="ACHJ48_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO4710158.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            1989..2258
                     /locus_tag="ACHJ48_004794"
     CDS             1989..2258
                     /locus_tag="ACHJ48_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4710159.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            2279..2923
                     /locus_tag="ACHJ48_004795"
     CDS             2279..2923
                     /locus_tag="ACHJ48_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710160.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            3239..3576
                     /locus_tag="ACHJ48_004796"
                     /pseudo
     CDS             3239..3576
                     /locus_tag="ACHJ48_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          951 a          753 c          979 g          893 t
ORIGIN      
        1 taatgaagtt tgcctgaatg tataaacaga attattcgac ataaagccag gaaaatgagt
       61 gatacgcttt taaccgaaaa aatccttacc ggagaaaatg ttttgcgtgc tgcaatcgcc
      121 cgcattgagt ggatttttga aacatttccg tcggtatgtc tgtctttttc aggtggaaag
      181 gattccaccg tgcttttcca tcttgtggca gaggtggccc gcaggagaaa acgtcatttc
      241 tctgttctgt tcattgactg ggaggcccag tatcggtgca ccattgaaca tattcagaag
      301 atgcgggaaa tgtaccatga tgtgacggaa accttttact gggtggcact tcccctgacc
      361 acggtaaacg gcgtctcgca gtttcagccg gagtggatat gctgggagcc aggagtgacc
      421 tgggttcgcc agccaccgga agaggccatt acggatatga catattttcc ctttttccgg
      481 tatgccatga cgtttgaaga atttgttccg gcattttctt cctggtttgc cggtaaccgg
      541 tgtggagtgg cagtgctgac cggtgttcgt gcggatgaat ccctcaatcg ctttatgggg
      601 ctggtgtctc agcgcaaact gagatatgcc gatgataagc cctggaccac ggcatcgcct
      661 gaagggtttt attacaccat gtatccgttg tatgactgga aagtccgcga tatctggata
      721 tataacgcca gagcttgtgc catttacaat cctctgtatg acctgatgta ccgtgccgac
      781 gtgccgttac gcaacatgcg tgtctgtgaa ccttttggtc ctgaacaacg taaggggctg
      841 tggctttacc atgttctgga gccggaaacc tgggccagga tgtgtgagcg ggtgtcaggc
      901 gctgccagcg gggcgcttta tgccaatgaa agcggtgcct attttgccct gcgtaaacgt
      961 atatcaaaac cagcccatca tacctggcgc agctatgcga tgttcctgct ggatgtgatg
     1021 ccggaaagaa cggcagaaca ttaccgtaat aaaattgctg tctacctgcg ctggtatcag
     1081 acgcggggct tcccggatga catcccggat gaacaggaga atgacctggg gagccgggat
     1141 attccgtcct ggcggcgtat ctgtaagaca cttataaaga atgatttctg gtgccggccc
     1201 ctctccttca gtccgaacaa accccggcac tatgaacgtt atctgcagcg tatgaaagaa
     1261 aggaggaagg aatgggggat tctgtgacac cggagataat tggcaacatg attcgtcagt
     1321 acttcagcca ggaacgacca gaagaagaga ccatacaggc ccttaatcac ctgcgcagag
     1381 tgcttcatga agtcagtcct tttgcacatg agccagtgga ctgtgtgctg tgggtaaaag
     1441 cagatgaggt ggtggccaat gattacaatc ctaatgtcat ggcgccgggt gagaagaaac
     1501 tgctgaagca gtcactggag aaagatggtt tcacacaacc ggtggtggtg tctgaggaaa
     1561 agaaccatta cctggtggtg gatggttttc atcgccaggt gctgggcagg gaagcaggta
     1621 cgggaaaacg tctgaagggc tggctgccgg tggtctgtat taatccggag agaaagggac
     1681 aggcctcacg tattgctgcg accatccgac ataaccgggc caggggcaaa catcagataa
     1741 cctcaatgtc tgatattgtc agggacctgt cgcgcctggg ctggacgaat gaacgcattg
     1801 gtacggagct gggtatggac caggatgaag ttctcagatt gaaacagatt agcgggctga
     1861 cggagctgtt tcaggaagag gatttcagcc cggcatggac ggttcgctga gaccgtcaga
     1921 tatgtatatt atttcagtga gttaatgttt tgagcaggaa aaagagtacc ccggaagttg
     1981 tggaagacat ggttgcgcag aaactggagg ctgctggttg ctggcgcaga gcttctgacc
     2041 gctggctttt tgttatggga aatgttgaat gtacagaagc acaacgggag tggttgttgt
     2101 tgcgccggaa ttattgtctg gcgcagatat cgtccccgcc gctacctgaa acgctggata
     2161 tcagcgaggt ggcgaaagct gcagatgcca cgctgaggcg aatggggatt gccactccgt
     2221 ccggggaggt attcagaaaa ggtacaccgg tctgttgata ttcaggtaac aggtattaat
     2281 gatgaagcaa ggaatggcaa acagtatatc aatgacatgg caacttttat gcgatgaaca
     2341 tggttttacc cgggagcact acaatcagct gaagaagttc agcccggaga cattacgtga
     2401 aattattgct gaaatagcca gctgtcatcc atcaaccagt gtattgctca ggaataagtg
     2461 gctgacaccg ccggaggata ttctggagca gattacccgt gaatacgaac gaagaataca
     2521 aaactgtcca ccgttcagga gcgagaagga agcggaatcc tggttaaatg aactatcttt
     2581 tgctgtgatt gctccgctat accgtgttgc ccccgaacaa acagaacagg ttgagtcctt
     2641 tgttctgcag cttattgccg agcaggagcg ggtttgggag cttattttaa ccaatgatgg
     2701 ttattcatgg cattgcgcac tttatgatat attatttcat ctggttttac gaaatatgga
     2761 agaccagcct gaacatcaga aacagcgaat aacaaaaata ttttatgagc ccgatgcaag
     2821 actggttgca gaagagatta aattccggat tcaatctctg tatgatgatg aacaaaagag
     2881 agctttaagt gaactggtaa atgattttac ttcaaaagaa taaaatgcgg cgaggtgatt
     2941 tttactatat gttaataaaa cggggggtag atgaaagtga atggtgttac tacatcaaaa
     3001 tatgaaggat ggcgagggac gtttcagtta tatagatata aaacggtagt tctggtgcac
     3061 cccaaaggat atctggcagg ggaaatgatt catcgccagg ccctaacgtc gtcttgtcaa
     3121 gattaatgct atagacatag ctaaattttt tatggataag gatatgaact gtgcctcagg
     3181 tccggaacat cctgtaggga ctttttaagt cgggattgag ctgaactaat gccaaattat
     3241 gaacagggaa attcagcagc ggctacagtg ggtaaaactc tatgaaacgt ccggtgatgc
     3301 gggatttgta tgccgtcgct gtggcatttc tcggcccaca ttgcgtaaaa tggtggcgca
     3361 gatatctggc tcagggcatc gctggtctgg aaagccacag ccgtcgcccc aagcgttcac
     3421 catcaacgaa aaccggcact tgcgaagtcg ctctgattct ggagttacgt tctccgcgaa
     3481 atctgggggc aagacgtatt caaagcgagt taaaacgact tcattcaatc tcactggcaa
     3541 tagcaacaat ccacaaagtc cttttccaga atcagg
//
LOCUS       DAZFDC010000249         3461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFDC010000249 DAZFDC010000000
VERSION     DAZFDC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.251"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(4..79)
                     /locus_tag="ACHJ48_004797"
     tRNA            complement(4..79)
                     /locus_tag="ACHJ48_004797"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Val,seq:tac)
     gene            complement(126..201)
                     /locus_tag="ACHJ48_004798"
     tRNA            complement(126..201)
                     /locus_tag="ACHJ48_004798"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Val,seq:tac)
     gene            complement(246..321)
                     /locus_tag="ACHJ48_004799"
     tRNA            complement(246..321)
                     /locus_tag="ACHJ48_004799"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:Val,seq:tac)
     gene            580..1995
                     /gene="gltX"
                     /locus_tag="ACHJ48_004800"
     CDS             580..1995
                     /gene="gltX"
                     /locus_tag="ACHJ48_004800"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO4710161.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(2113..2538)
                     /gene="tnpA"
                     /locus_tag="ACHJ48_004801"
                     /pseudo
     CDS             complement(2113..2538)
                     /gene="tnpA"
                     /locus_tag="ACHJ48_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            2546..>3461
                     /locus_tag="ACHJ48_004802"
     CDS             2546..>3461
                     /locus_tag="ACHJ48_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO4710162.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKELERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYLNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCTANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWY"
BASE COUNT          906 a          846 c          904 g          805 t
ORIGIN      
        1 aagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc
       61 ccagctgagc taatcaccca cttcggtact tcacattttt acaagaaatt tagctggtga
      121 gaaagtggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc
      181 tcccagctga gctaatcacc cacttctcaa tttcttgcta cacggcggag actacataaa
      241 gtagttggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc
      301 tcccagctga gctaatcacc cccgctgtgt ggagtcgcat tatagggaga gttcaaaatg
      361 agtcaacgca ttttctaaag aaattgttcg ttcgtcgtaa atttaagcaa gatgatcgca
      421 aaacagaccg tgttgcgcaa tttgtcaacg aaaacaataa tgcgtaaggt tgcaacccga
      481 actacattga ggaatcaggc gggagtgata gaatatcgcc cactcaattt ttccaggatt
      541 tgccggttgt cggcatcttt ctaaacgtaa ggccatttca tgaaaatcaa aactcgcttc
      601 gcgccaagcc caacaggcta tctgcacgtt ggcggcgcgc gtactgctct ttactcctgg
      661 ctttttgcac gtaatcacgg cggtgagttc gtgctgcgta ttgaagacac cgatcttgag
      721 cgttccacgc cggaagctat cgaagccatt atggatggca tgaactggct gagcctggag
      781 tgggatgaag gtccgtactt ccagaccaaa cgttttgatc gctacaacgc ggtgatcgat
      841 cagatgctgg aagagggcac agcttataaa tgctattgct ctaaagagcg cctggaagcg
      901 ctgcgcgaag agcaaatggc gaaaggtgag aagccgcgtt atgacggtcg ctgccgccac
      961 agtcatgagc atcacgctga tgatgagccg tgtgtcgtac gttttgctaa cccgcaggaa
     1021 ggttctgttg tttttgacga tcagatccgt ggtccgatcg agttcagcaa ccaggaactg
     1081 gacgatctta ttatccgccg taccgatggt tccccaacct ataacttctg tgtggttgtc
     1141 gatgactggg atatggaaat cacccacgtt atccgtggcg aagaccatat caacaacacg
     1201 ccacgccaga tcaacattct taaagccctg aaagcgccgg taccggttta cgcgcacgtt
     1261 tctatgatca acggcgatga cggcaaaaaa ctgtccaaac gtcacggggc agtcagcgta
     1321 atgcagtatc gtgatgacgg ttatttgcca gaagcactgc tgaactatct ggtgcgtctg
     1381 ggctggtccc acggcgatca ggaaatcttc actcgtgaag agatgatcaa atacttcact
     1441 ttgaatgccg tcagcaaatc tgccagtgcg ttcaacaccg acaagctgct gtggctgaac
     1501 catcactaca ttaacgcgct gccgccggag tatgttgcta ctcacttaca gtggcacatt
     1561 gagcaggaaa atatcgatac ccgtaacggc ccgcagctgg ctgatctggt gaaactgctg
     1621 ggcgaacgct gcaagacgct gaaagagatg gcacagagct gccgttattt ctacgaagat
     1681 tttgctgagt tcgatgccga cgccgcgaaa aaacatctgc gtccggtagc gcgtcagccg
     1741 ctggaagtgg ttcgtgacaa actggccgcg attactgact ggacagctga aaacgttcat
     1801 cacgctattc aggcgacggc ggatgagctg gaagtgggta tgggtaaagt tggtatgccg
     1861 ctgcgtgtcg ccgtaaccgg tgcggggcag tctccggcgc tggacgttac cgttcacgcg
     1921 atcggtaaga cccgcagtat cgagcgtatc aacaaagcgc tggcttttat tgcggaacgc
     1981 gaaaatcagc agtaattagc gcataaaaga taaacggcag gagagatacc cctgccgttt
     2041 tttatttatc cggtgcgccg taaaaccccg tccttcaggg cggggatata aggcgcggtt
     2101 ttccacctga ccaggtgttt gctgttgttc aatgtattgg cggatgacgg atattggcgc
     2161 acctccgcag ctactggcaa aatagccagg actccacaaa acgcctttgt agtaatacct
     2221 gactgcaata tctggtcgat ctcgtcgcag taacctaccc gatacgcctt tgaggctgtt
     2281 taccagactg gatatcgcca gtttgggagg atagttagtt aacaaatgga cgtgatctgg
     2341 ttcgccatcc atttcaacca gttcagcttc aaaatcagca catacatttg aaaagtaagt
     2401 gcgtagtttt tctgtcgcgt catagtcaaa aatctgacgt cggtatctgg tgacaaagaa
     2461 cagatgaaca tgcatcagga aaacacaatg ccttccccgt cggatatcgg tttctttatt
     2521 catagaccaa agtatcattt ggcctgtgaa acgactacag gcatttaaat tccagttaag
     2581 accaggtggt caacaggagc gtgaaatgag gcgcttcgca ggcgcatgtc gtttcgtttt
     2641 caatcgtgct ctggcgcttc agaatgagaa tcatgaggcc ggtaataaat acatccctta
     2701 cgggaaaatg gcttcctggc tggttgagtg gaaaaacgcc actgaaacgc aatggcttaa
     2761 agatgccccg tcacagccat tgcaacagtc actgaaagag cttgagcggg cttacaaaaa
     2821 cttcttccgg aagcgggctg cttttccccg attcaaaaag cggggacaga atgatgcatt
     2881 ccgctacccg cagggcgtta agctcgatca ggaaaacagc cgtatttttc tgccgaaact
     2941 tggctggatg cgctacctga acagccgtca ggtcacgggt gttgtgaaaa atgtcactgt
     3001 cagccagtcc tgcggtaagt ggtacatcag tattcagaca gaaagtgaag tatcaactcc
     3061 ggttcaccct tcagcatcaa tggtcggact ggatgctggc gtggctaaac tcgccacgct
     3121 gtcagatggc acagtctttg agcctgtaaa cagttttcag aaaaaccaga agaagttggc
     3181 gagacttcag cgacagttaa gccgcaaggt caaattcagc aataactggc agaagcagaa
     3241 acgcaaaata cagcgactgc attcctgtac cgcaaatatc cgcagggact accttcacaa
     3301 agtcacaacg accgtcagca aaaaccacgc aatgatagtc attgaggatt tgaaggtcag
     3361 caacatgtca aagtcagcag cgggtacggt cagccagccg gggcgcaatg tccgggcaaa
     3421 atcaggttta aaccgttcga tactggatca gggctggtat g
//
LOCUS       DAZFDC010000250         3435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFDC010000250 DAZFDC010000000
VERSION     DAZFDC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.252"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(612..809)
                     /locus_tag="ACHJ48_004803"
     CDS             complement(612..809)
                     /locus_tag="ACHJ48_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4710163.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(875..1145)
                     /locus_tag="ACHJ48_004804"
                     /pseudo
     CDS             complement(875..1145)
                     /locus_tag="ACHJ48_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1450..1638
                     /locus_tag="ACHJ48_004805"
     CDS             1450..1638
                     /locus_tag="ACHJ48_004805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710164.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1966..2160
                     /locus_tag="ACHJ48_004806"
     CDS             1966..2160
                     /locus_tag="ACHJ48_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710165.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2503..2631
                     /locus_tag="ACHJ48_004807"
     CDS             2503..2631
                     /locus_tag="ACHJ48_004807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710166.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2941..3276)
                     /locus_tag="ACHJ48_004808"
     CDS             complement(2941..3276)
                     /locus_tag="ACHJ48_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710167.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1028 a          777 c          743 g          887 t
ORIGIN      
        1 gattcaggat acagtttgta ggagctttct tttttgaaca atgatttgca ggacagttat
       61 gctaaactag cggaatcagt atcacaacaa taaatgactg atcatacctg aaatttgaat
      121 taacaggcat catgaatgtc aatagtctaa gcggtgacta cttatatcgc ttgcataaat
      181 actaaacact gactagaaaa gtattggata accccgtcac taaaagtaac gcttcggtta
      241 tttgctcgga atgtattaac gccaagattg ttagccgtta cgccttatat cactggcgct
      301 gacaaatgtt tcttttctaa tttactgaac cggcgcagac acgcaggaaa gcacgccggc
      361 accggtgtaa tgccagttct aatcaataag aatggaatga cgaatcagtc aggaagggat
      421 aacaacccgc tgctgaacgg aaggtatcag caggaagatg aacgggaata tcgccagcac
      481 caagggcagg gccgggttcc acaataccag cgctggcatt ttgcacattg ccagcgtacc
      541 ggcattacaa cacgtttcat gctgctgccc cccaggattc ctcgattcgt tgctacatcc
      601 actgctctac ttcacttcca ctgctccact tcacttttgt accagcggct gctgcgcccc
      661 agtttgatgg gtttagggaa atggccttca ctgatcaact tgtaaaacca tttgtcggtc
      721 ataccggtga aactggtgat atagttcatg tcgatcaggg aattatcggc acgcagaaat
      781 acgctgtcgt ggcttgttgt agtcaacatg gtcagaatgt cctgtgagat ttatgaagat
      841 tgtgcgaatg cgggaaatct actgagctgt gctttcagaa ctggcctgtt acgggatacc
      901 agggattacc ggcggggtaa cgggcttccg gatcatacac accacgatta tcgcggacaa
      961 aatcactgaa cgcccatatc acctctttaa gtatgtcttc gcagtccggt aaatgacgat
     1021 ccagcgccac atcccgggtg gtacagcttt gatgcgcccg gtgacacaaa gcccggattg
     1081 ttccagacat cctgaatcaa acgccccaga ttaggggcgt cgaaatatgc ctctctgacc
     1141 attatattcc ggcgtatagg tagcaggtct gaagtgacaa tgcgtcacct gacgttaaaa
     1201 gtcacccaag atgaccccag tgggggcaac aagtataaaa ccgttttttg attaagtggt
     1261 cggatatgaa aaggggaagg tttatgtgaa aaatacctgt gttttctttg aaaacacaca
     1321 acagtatcag gttatttatg tttatacagt gcgttccaaa ctgacctgaa taattacgca
     1381 agttgcaaca ataaaacatt tttgccgtag tgtgaggtac tccggtaaat tgcttcccag
     1441 gtgcagacaa tgactaacga agaactacag gcaatcgcat tatccatgct tgagcgccag
     1501 cgcgaaaaag tacaccagca acgagagtct ttgcttaacc catctccatt caccaggcca
     1561 aaacgattgg agcttgaagc attcttggac aaatacccac ggcggcttaa atcaggcaaa
     1621 accagaccac caggctgata gctcacagtt tgttctccga ttcaataaaa cggataagaa
     1681 ccatcagcgc ggtagcgtaa ggcactacag cgcattgctt tacgagtaat aacaatggat
     1741 ttatcaccag aagacgcaaa gtttttacga gaactgcaac accgtatgct cagagaagcc
     1801 attatcgaac agcaaacggg catgagcgcc atagacccat tgcgatacaa acgggcctca
     1861 tctcctttag ttgacgacaa ttccaatgcg gataaaacac gtccaccaca ctgattacat
     1921 ttttcattag cacagtggtg tgatgcagca tacgaggaaa accggatgaa tgagaacaaa
     1981 ataaaaaggc tggagcaact gttacaggca cgccaacggg agtttgccac taaatgggga
     2041 cgtgccgcgc tgccacatga acgcctaacc tattttccac ttgaagacct cattaaaaat
     2101 aatggcaaaa aaatcaggtc tgataagaaa cacttcttag aatgcaatga agacaaataa
     2161 caacaatctg catattgcca ccaaccacag atgtggcatc gttatcagcg cacttgattt
     2221 tctttatatc cactgacgag tttgccggtc attctggtat catgcagata tttacggcgt
     2281 acaagcacac tagcatgaca ttgtaataag gcctgagcct aacgctttaa tgtgtgtcat
     2341 acactcagtg gcgtaatcgt tgacaatatg gtgcatgtaa tcgtaagcgt ccagcgaaat
     2401 ccatctggtc ataatctgaa gcatccgaca aagtggtgtc caccaaataa gtgatctgcg
     2461 cccaatatcc tgagccggaa ctttgacaaa ggccaacgaa aaatgggagc ggatgttaca
     2521 gagttctcgg tacaggggaa aagtggtacc tgtcgccagt tctccgatct gtttaaccgg
     2581 gaaattatct cctacagctt gtcagaaagg tcggtgatgg agcatcgtta atacgatgct
     2641 atacgatgca ttcacagtgc ccggccagag gatgccccgt cgctgcatat ggatcagggt
     2701 tggcaatatc gaattgcagg ctataggcaa agttaaagcc ccatggagta gcacaaaata
     2761 tgccgcacaa aggaaacggt ctgaataacg cagtgatgaa gaacttcttc agcacactgc
     2821 taaaacgcat agtgatcgag cgctgattct ggcgaacaac tgaactaata catcagaatc
     2881 tgcattatgt taaataaata taaaaagatg gtttaaatac cccgttactt ttgacttaca
     2941 ctatacggta tcgcatcgtt taatattcgc accggccaga tttttatttc tattagttgt
     3001 cacaatactg aatgcgtacg accacagtat tctggctcct gtgtggttat gctttaattc
     3061 tgcgttccgg gcagataagc agttgcttgc aggaatcctt cttgtgttaa tgtcagttcc
     3121 ccttttacca gtgctgattt ccacattccg tccaacagag cttatagcct ttccctggat
     3181 tatagcattg tccggctgaa gttctttttg aataataata gaagcactgc tggcagatcc
     3241 agtccgtttt tcataaccca ctgtactgat aaccataatc taatcagtag aaattgagtc
     3301 gaaaataagc actactccat acaggataat tagaggtcag tttgattatt cacaattcat
     3361 catcagcatt ttctatttct gacgaaatca atatgaaaat aaccatatat gataattatt
     3421 ataataacgg cttta
//
LOCUS       DAZFDC010000251         3352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFDC010000251 DAZFDC010000000
VERSION     DAZFDC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.253"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1400
                     /gene="escC"
                     /locus_tag="ACHJ48_004809"
     CDS             <1..1400
                     /gene="escC"
                     /locus_tag="ACHJ48_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO4710168.1"
                     /translation="DFAANYSIPVFISSSVNDDFSGEIKNEKPVKVLEKLSKLYHLTW
                     YYDENILYIYKTNEISRSIITPTYLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEV
                     RGVPECIKYITSLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGV
                     VSILKTMASNGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQL
                     ISELDIEQRQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSV
                     ISNASNFMIRVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLES
                     ITSGTLLRVTPRILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQ
                     NSEMTTEATLSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVR
                     LFLIKATPIKSASSE"
     gene            1414..1869
                     /gene="cesD"
                     /locus_tag="ACHJ48_004810"
     CDS             1414..1869
                     /gene="cesD"
                     /locus_tag="ACHJ48_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO4710169.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(2253..2666)
                     /gene="grlA"
                     /locus_tag="ACHJ48_004811"
     CDS             complement(2253..2666)
                     /gene="grlA"
                     /locus_tag="ACHJ48_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO4710170.1"
                     /translation="MESKNKNGDYVIPDSVKNYDGEPLYILVSLWCKLQEKWISRNDI
                     AEAFGINLRRASFIITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIES
                     PGTTGPKRKTYRVGNGIVGQSNIWNEMIMRRKKES"
     gene            complement(2721..3092)
                     /gene="grlR"
                     /locus_tag="ACHJ48_004812"
     CDS             complement(2721..3092)
                     /gene="grlR"
                     /locus_tag="ACHJ48_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO4710171.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNMITGGDIASVYQGVL
                     SEDEDIILHVHRYNYEIPSVLNIEQDYQLVIPKKVLSNDNNLTLHCHVRGNEKLFVDV
                     YAKFIEPLVIKNTGMPQVYLK"
     gene            3288..>3352
                     /locus_tag="ACHJ48_004813"
     CDS             3288..>3352
                     /locus_tag="ACHJ48_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HFO4710172.1"
                     /translation="MKKIILSIILISNYCYASDCF"
BASE COUNT         1115 a          635 c          555 g         1047 t
ORIGIN      
        1 atgactttgc tgctaactac agcattcctg ttttcatcag tagttccgtg aatgatgatt
       61 tctctggcga aataaaaaat gaaaaacctg taaaagtact ggaaaaactg tccaaactat
      121 atcatctcac atggtattat gatgagaaca tcttgtatat ttataaaaca aatgaaatat
      181 caagaagtat aattacacca acatatctgg atattgactc actgctaaaa tatctgagtg
      241 atacaatctc agtaaacaaa aactcttgca atgtgagaaa aattacgaca tttaactcga
      301 ttgaagttcg gggtgttcct gaatgtataa aatatattac atctttatca gaaagtcttg
      361 ataaagaggc acagtctaaa gccaaaaata aagatgttgt taaggtcttt aaactgaact
      421 atgcgtcagc gacagatata acatacaaat accgtgacca aaatgttgtc gtcccaggtg
      481 ttgtctccat tctgaagaca atggcaagta atggttcact tcccagtacc ggtaaaggtg
      541 ccgtcgagcg aagcggtaac ctgtttgata acagtgtaac gatttccgct gatccacgtc
      601 ttaatgcagt tgttgtaaaa gaccgcgaaa taacgatgga tatctatcag caattaattt
      661 ctgagcttga tattgaacaa aggcaaattg aaatttctgt ctcaattatt gatgttgatg
      721 caaacgatct tcagcagctc ggcgtaaact ggtccggtac gttaaatgca ggacaaggta
      781 cgattgcctt taatagctct accgcacagg ctaatatcag ctcttctgtg ataagtaatg
      841 ccagtaattt tatgattagg gtgaatgcgt tacaacagaa ctcgaaagct aaaatactct
      901 ctcagccttc aattataacc ttaaataata tgcaggcgat tcttgataaa aacgtaacgt
      961 tttatacaaa agtctccgga gaaaaggtgg cgtctctgga aagtattaca agcggaacat
     1021 tgctgagagt gacaccacgt atacttgatg acagctcgaa tagccttacc ggaaaacgtc
     1081 gggaacgagt tcgtttgtta cttgatattc aggatggcaa tcaatcgact aaccaaagca
     1141 atgctcagga tgccagcagc accttacctg aagtacaaaa ttcagaaatg acaacagaag
     1201 cgacattaag tgcaggagaa agtttattac ttggcggatt tatccaagat aaagaaagtt
     1261 caagtaaaga tggtataccg ctgttaagcg acattcctgt tattggtagt ttattctcca
     1321 gtacggtaaa acaaaaacat agtgttgttc gactcttttt aattaaagca accccgataa
     1381 aatctgcatc tagcgaataa taaatgaggc tttatgagca ggaaatttag ctctctagag
     1441 gatatttatg atttctacca ggatggtggc acattagcgt cattaacaaa tctgacacaa
     1501 caagatctca atgaccttca ttcttatgcc tatacagcat atcaatctgg tgatgttata
     1561 accgcaagaa atctattcca tttgctcaca tatctggaac actggaatta tgactacacc
     1621 ttatctctgg gcttatgtca tcagcgttta tcaaatcatg aagatgcaca actgtgtttc
     1681 gcacgctgtg caactttagt tatgcaagat cccagggcat cttattattc tggaattagc
     1741 tacttactcg tcggaaataa gaaaatggcc aagaaagcct ttaaggcttg tttaatgtgg
     1801 tgtaatgaaa aagaaaaata cactacatat aaagaaaata ttaaaaaatt gttaggtaat
     1861 acagagtaaa aactaccatc agctatacca atccccaggc tgactgggga ttttctatcg
     1921 gaaataataa agctgctata gtgaagtgct cccaaagttg agcaccgacc actggtagat
     1981 ttatctacag gtgtggggtt attccattaa ccaggagttt atgccctgaa ctatcttctg
     2041 ctgtatttaa tatcatttcc gtttatgaaa caatcagtat atcttaactt agcattagaa
     2101 atctgatctt tccaatcatc tatagctcag agtatatttg tcaggaaatg accttcaaac
     2161 tttagccgag aaaaaggctt accctggaaa acaaaaccct taaatatagc tttattttta
     2221 ttcttctata aaatatactc aaaaaattac gtctaactct cctttttccg cctcatgatc
     2281 atttcgttcc agatattaga ctgtcccaca ataccattac caactcggta ggtttttctt
     2341 tttggtccgg ttgttccagg actttctatc ggaaccgcct caaggttaac atcataaatg
     2401 aaaatctcaa ggcgcttata atgcaaatta ccataactaa catatctgac acgaaatgaa
     2461 attttttctt ttcttctcga tatataagtt ataataaatg atgctctcct caggtttata
     2521 ccgaatgctt cggcaatatc attgcgagaa atccatttct cctgcaattt acaccaaaga
     2581 gaaaccaaga tatacagagg ttcaccatcg taattcttta ctgagtcagg aattacatag
     2641 tcgccatttt tatttttaga ttccattttc catattcttt ttatttgata aataaatcga
     2701 cataaaaaac atacataaaa ttattttaaa taaacttgtg gcattcctgt gtttttaata
     2761 actaatggtt ctataaattt ggcataaaca tcaacaaaca atttttcatt tcctcttaca
     2821 tggcaatgta atgtgagatt attatcatta ctcagtactt ttttagggat aactaattga
     2881 taatcttgtt caatgtttag caccgaggga atttcgtaat tatatcgatg gacatgaagt
     2941 atgatgtcct catcttcaga gaggaccccc tgatacacag aagcaatatc gccgccagtg
     3001 atcatatttc catttttaat cagtatacct tccccacagg agtcttcatt gctaataaat
     3061 ataatgctat agatgccatc cttcataatc ataactcact cagtttcata ctaacctcac
     3121 tcctttcaat ttgttctatc cataagcaat atcaagaata atggagacct ttctttttct
     3181 ccagattgca atgtaatata tcattaaata tactcatgtt tcaacctgct gtttccctgg
     3241 ttaacatatg taatttctga ttaataatat tgagtaacaa cgtaaaaatg aaaaaaataa
     3301 tactgagcat cattctcatt tcaaattact gttatgcttc cgattgtttt ga
//
LOCUS       DAZFDC010000252         3347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFDC010000252 DAZFDC010000000
VERSION     DAZFDC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.254"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..457)
                     /locus_tag="ACHJ48_004814"
     CDS             complement(<1..457)
                     /locus_tag="ACHJ48_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4710173.1"
                     /translation="MRRKREEIASPEATQRLRAIWDAKKRELKLTQELAAELMGFEAQ
                     SAVSHYLNGKAPLNTDAALKFAVLLRVKPEELRPDLADLMNYVRSSGTYDESFEGEGW
                     RMVNKQQADLLDLFDILPESEKAKLIERLKGQNELYKEAFENMLAAQKRM"
     gene            562..837
                     /locus_tag="ACHJ48_004815"
     CDS             562..837
                     /locus_tag="ACHJ48_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4710174.1"
                     /translation="MTLDEYLKKNRVRQSCLAALAGCSQSMISLVATGRSQLSPEKVL
                     RIAEATNFEVTPHELRPDIYPNPTDGLPVGCKANTQNAQELIHENQA"
     gene            821..1243
                     /locus_tag="ACHJ48_004816"
     CDS             821..1243
                     /locus_tag="ACHJ48_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4710175.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAIIPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1321..2109
                     /locus_tag="ACHJ48_004817"
     CDS             1321..2109
                     /locus_tag="ACHJ48_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710176.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMAGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKCNAASVTQTDQKALPEEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIDLPDWLPETLWHEWVRFRQALRKPIRTEQGANGAIRELEKFRQQGFTPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            2116..2856
                     /locus_tag="ACHJ48_004818"
     CDS             2116..2856
                     /locus_tag="ACHJ48_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO4710177.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLTEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGYT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASLRSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            2882..>3347
                     /locus_tag="ACHJ48_004819"
     CDS             2882..>3347
                     /locus_tag="ACHJ48_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4710178.1"
                     /translation="METVLHALKAMGKANSVELAARIDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESRVTEERPVKSETQDMLTEEVAPKVSANMMIEFIAQEGAKTCEEI
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGRFRYCMPGDNLPAEPKAALVTE"
BASE COUNT          904 a          736 c          895 g          812 t
ORIGIN      
        1 tcatgcgttt ttgagctgcc agcatatttt caaatgcttc tttgtagagt tcattctgac
       61 ctttaagtct ttcgattagt tttgcttttt ctgattcggg aaggatatca aaaagatcta
      121 gtaaatcagc ttgttgcttg ttaaccatcc gccaaccttc gccttcgaaa ctttcgtcat
      181 aagttcctga ggaacggacg taattcatca gatcagctaa atcaggcctc aactcttcag
      241 gcttaactct tagtaataca gcgaatttta atgccgcatc agtattaagt ggagcctttc
      301 cgttgagata atggctaacc gcagattgcg cctcaaaacc cattagctcg gcagcaagct
      361 cctgagtcag tttgagctct cttttttttg catcccagat tgcgcgcaga cgttgcgtag
      421 cttccggcga tgcgatttct tcgcgttttc ttctcatacc accatcttat gaatacagtt
      481 cataatctca aactgatata ggtattgatc atttaaatta gtatggttaa tatttttgtg
      541 agcattacta aggtgaccat tatgacatta gatgaatatt tgaaaaaaaa tcgtgtacga
      601 cagtcttgtt tggccgcgct ggctggttgt tcgcaatcaa tgattagcct cgttgctact
      661 ggacgtagtc agttaagccc tgaaaaggta ttgcgtatcg cagaggctac gaatttcgag
      721 gttacacctc atgaactccg gcctgatatc taccctaatc cgaccgatgg tttacctgtt
      781 ggatgtaagg ctaacacgca aaatgcacag gagttgattc atgaaaatca ggcatgagca
      841 catcgaatca gtgttgttag ccctggcagc cgaaaaaggg caggcgtggg tcgctaacgc
      901 aattactgaa gaatatctgc gccagggggg cggcgaattg cccctggtac caggcaagga
      961 ctggaacaat cagcagaata tctatcaccg ttggttgaaa ggtgaaacga aagcgcaaag
     1021 ggaaaaaatt cagaaactga tccctgcggt tctggcaatt attccgcgcg agctgcgtca
     1081 ccgactctgc atcttcgata ccctggaacg ccgtgcatta ctggcggcgc aggaagcgtt
     1141 gagtacggca attgatgcgc atgatgatgc agtccaggcc gtttaccgga aagcacattt
     1201 cagcggtggt gggtcgcccg gcgattctgt cgtagtgcat tgattgaaat taatcgtgcc
     1261 ggattgtttt gttcggtatc agttaaatgt aacgctgcga gcgttacaag gtgaaaacaa
     1321 atggcttcaa actggataaa gctcgaggtt attacgccgg ataagccgga aatattcagg
     1381 cttgctgaga ttctgaatat tgatccagat gccgcattag ggaaggttat tcgcttctgg
     1441 gcatgggcgg atcaacaaat gatagacggt aatgcagatt gtaacgctcg cggcgttaca
     1501 aaaagtgcaa tagatcgcat cacttttatg gctggttttg ctgatgcgtt aattcaggtt
     1561 ggatggctgg tcgaaaatga cggtgggctt tctctaccta actttgaacg tcataacgga
     1621 aaaagctcta aaaaacgggc ggttacaaac gagcgagtta caaaaatacg cgaactgaaa
     1681 cgaaaatgta acgctgccag cgttacacaa acggatcaaa aagcgttacc agaggaagag
     1741 gaagaggaag atctaaatac tgatctcccc ctaaatcccc ctcgccaaaa acgagcgtct
     1801 aaaaaattcg agccggaggc tattgatctg cccgattggt tgccggaaac actctggcat
     1861 gagtgggtcc ggttcagaca ggcattgcga aaaccgattc gaacggagca gggcgctaac
     1921 ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga gcaggtgatt
     1981 cgacacagca tcgccaatga ataccagggc ttgttcgcac cgaaaggtgt tcggcctgag
     2041 acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc gccaggcttc
     2101 agggggtaac agaccatgaa aaatattgcg acaggcggcg ttctggagcg tatccgcaga
     2161 ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg tgagtggcaa
     2221 cttactgaag gccagaaacg ttgcgaggag atcaaccgtc tgaatcgtca gttgcgggtg
     2281 gaaaaaattc tgaatcgctc tggcatccag ccgttgcacc gtaaatgctc gtttgcgaat
     2341 taccaggtgc agaacgacgg ccagcgatac gcgttaagcc aggcgaaatc catcgctgat
     2401 gaactgatga ccgggtatac aaattttgcg ttcagcggaa aacctggtac cgggaagaat
     2461 cacttagcgg cagctatcgg gaatcgcctg ctgaaagacg gtcagacagt gattgtggtt
     2521 accgtggctg atgttatgag cgccctgcac gccagctatg acgacgggca gtcaggcgaa
     2581 aaatttttgc gtgaactgtg cgaagtggac ctcctggttc ttgacgaaat tggcatccag
     2641 cgtgagacga aaaacgagca ggtggtactg caccagattg ttgaccgccg gacggcatca
     2701 ctgcgcagtg tcgggatgct gacaaacctg aatcatgccg caatgagcac gcttcttggt
     2761 gagaggatta tggaccggat gaccatgaac ggtggtcgtt gggtgaattt taactgggag
     2821 agctggcggt caaacgttgg acgtcagggt atgtgagaat ttttaacgag gtgaattttc
     2881 gatggaaacc gtattacatg cactgaaagc gatgggaaaa gccaattctg ttgaactggc
     2941 ggcacgaatt gatatcagcc gtgaagaagt tctcaacgaa ctgtgggagc tcaaaaaaaa
     3001 tggcgttgtt gataaaacgg gtcacacctg gtttctggct gtcgaaggtg aatcccgggt
     3061 aaccgaagag cggccagtaa aatctgaaac acaggatatg ctgaccgaag aggtcgctcc
     3121 aaaagttagc gctaacatga tgattgagtt tatcgctcag gagggggcta aaacctgtga
     3181 agaaatagcg ggtaagttcg gtgtcagtac tcgcaaggtt gcttccacgc tggcggtggt
     3241 aaccgcaacg gggcggctgg cacgcgttaa tcagaacggt agatttcgtt actgcatgcc
     3301 gggcgataat ttaccagcag agccgaaagc cgcgctggta acggaaa
//
LOCUS       DAZFDC010000253         3306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFDC010000253 DAZFDC010000000
VERSION     DAZFDC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.255"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            24..1064
                     /gene="lsoA"
                     /locus_tag="ACHJ48_004820"
     CDS             24..1064
                     /gene="lsoA"
                     /locus_tag="ACHJ48_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="HFO4710179.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTY
                     KGIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDD
                     DRANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQ
                     IASLINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQL
                     SCSLIMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKY
                     IIREIHQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVC
                     QLIDGGVARLKL"
     gene            1061..1441
                     /gene="lsoB"
                     /locus_tag="ACHJ48_004821"
     CDS             1061..1441
                     /gene="lsoB"
                     /locus_tag="ACHJ48_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="HFO4710180.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKK
                     KSKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKK
                     NFDKINVNLLSKATSFALKKGIPI"
     gene            complement(1676..1999)
                     /gene="mobA"
                     /locus_tag="ACHJ48_004822"
     CDS             complement(1676..1999)
                     /gene="mobA"
                     /locus_tag="ACHJ48_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO4710181.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            2271..2465
                     /locus_tag="ACHJ48_004823"
     CDS             2271..2465
                     /locus_tag="ACHJ48_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO4710182.1"
                     /translation="MNKQQQTALNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTEI"
     gene            complement(2593..2841)
                     /locus_tag="ACHJ48_004824"
     CDS             complement(2593..2841)
                     /locus_tag="ACHJ48_004824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710183.1"
                     /translation="MAGSEQESARGSFRGETPGIFIVLSGFATSGLSVDFCDARQGGG
                     AYGKTPAVLASSGALLFAHMFFPALSPDSVDNRITAFE"
     gene            2952..3065
                     /locus_tag="ACHJ48_004825"
     CDS             2952..3065
                     /locus_tag="ACHJ48_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO4710184.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAAIGN"
BASE COUNT         1074 a          743 c          692 g          797 t
ORIGIN      
        1 aaaaaaaaca acggattgac ccaatggcac agaacccgtt taaagcacta aatatcaata
       61 ttgacaagat tgagtctgct ctgacgcaga atggcgtcac aaactattcc tctaatgtaa
      121 aaaacgaaag agaaactcac atatctggca catataaagg aatagacttc ttaataaaac
      181 taatgccatc aggcggaaat accactatcg gaagagcgtc tgggcaaaat aacacttact
      241 ttgatgaaat cgccttgatt ataaaagaaa actgtttgta ttcagacaca aagaactttg
      301 aatacaccat tccaaaattc agtgatgatg acagggcaaa tctatttgaa tttctttctg
      361 aagaggggat aacaataaca gaagataata ataacgatcc taattgtaaa caccaatata
      421 ttatgaccac cagcaatggt gacagggtca gggcaaaaat ttacaagcgc ggctctattc
      481 aattccaagg aaaatacctt caaatcgcga gtttgattaa cgatttcatg tgctcaatac
      541 taaacatgaa agagattgtc gaacaaaaaa ataaagaatt taatgttgac ataaaaaaag
      601 aaactattga gtccgagttg catagcaaac taccaaaaag catcgataaa atccatgaag
      661 atatcaaaaa acagctatca tgctcgctaa taatgaaaaa aatagatgtc gaaatggaag
      721 attactcaac atactgcttc tctgcattaa gagccataga aggctttata tatcaaatac
      781 ttaatgatgt ttgcaatcca tcatcatcaa agaaccttgg cgaatacttc actgaaaaca
      841 aacccaaata tataatcaga gaaatacacc aagaaactat aaatggtgaa atagcggaag
      901 ttttgtgtga atgctacact tactggcatg agaacaggca tggtttgttt catatgaaac
      961 caggaatagc tgacacgaag acaattaaca aattagaatc aatcgcaatc atcgataccg
     1021 tttgccaatt aatagatggt ggcgtagcta ggttgaaatt atgaaaaaag ataaaaaata
     1081 tcaaatagaa gcaataaaaa ataaagataa aactttattt attgtctatg ctactgatat
     1141 ttatagcccg agcgaatttt tctcaaaaat cgaatccgac ttgaagaaaa agaaaagcaa
     1201 gggtgatgtt ttttttgatt taataattcc taacggtgga aaaaaagatc gttacgtcta
     1261 tacgtcattt aatggcgaga agttttcaag ttacacatta aacaaagtta cgaaaactga
     1321 tgaatataat gatttatctg agctctcggc ttcgttcttt aaaaaaaact ttgataagat
     1381 caacgtaaac cttctatcca aagccacatc atttgctttg aaaaaaggca ttccaatata
     1441 atatcaagcc agaagacatt taccataacg tctcattacg cgcaccgcca aaacgcgctg
     1501 aacgcgattc tgacgcagac accgcaaaaa gagatgtttt ccccggggta aaccggaaaa
     1561 atgcgtcagg atcgctttca gctcactgca tagctatgca tgaaagtgaa tggcgatcgg
     1621 tttgggggct tacggcgttc ataccgtctg ttttcgacag tttctctccg ggaagctaat
     1681 ctgccataag cctggataac agggcacggt gatactccgt aatagcgatc agcacctccg
     1741 catactctct gtccccgaca cgcttgccgt cgataaagag ttttttctgc aacgccccca
     1801 gtcgcataac ttctttcagc acccggtcat cagtcagtga gttaactttc ttaccgagag
     1861 ctgccgcccg taaaaaacca gaaacggtct tgccgcattc agcagccctt tttctgattt
     1921 cctggtcttc ttctgccgtc agacgaacag ggcgactgat agcccgcctg cgcgtattac
     1981 ttccacttcg ttttgtcata aaacatggct ccgtatctga catggggtgt cggggcgaag
     2041 ccctgaccag ggtaattgta atagcgtgca tgtatgcgcg gtataacaat tgcacatcct
     2101 gtcctgtctg caagttcgaa ttaccaacaa agcactgctg ttttttggca gtgacagtgt
     2161 tgtctgactg aaatgtccgt cggcagaata ccggcggtaa gacggtcgaa aagtggtgcg
     2221 cgatataatc acacgacgca gatgacagac agaacggaga aaacagaaag atgaacaagc
     2281 agcagcaaac tgcactcaac atggcgggat tcataaaaag ccagagcctg acgctgctcg
     2341 aaaaactgga cgcactcgat gctgacgagc aggccaccat gtgtgagaag ctgcacgaac
     2401 tcgcagaaga actccagaac agcatacaga cacgctttga agtagaaaat agcacagaga
     2461 tataacgctc agggcaggat gccgaacggc acaacagagc gtaaggagaa aatgtcacat
     2521 aaggcgctct tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
     2581 agcggtgtca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataaagc
     2641 cggaaagaac atgtgagcaa aaagcaaagc accggaagaa gccagcaccg caggcgtttt
     2701 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagc cagaggtggc
     2761 gaaacccgac aggactataa agataccagg cgtttccccc cggaagctcc ctcgtgcgct
     2821 ctcctgttcc gaccctgcca cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2881 tggcgctttc tcatagctca cgctgttggt atctcagttc ggtgtaggtc gttcgctcca
     2941 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3001 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccattggta
     3061 actgatttag aggagtttgt cttgaagtta tgcacctgtt aaggctaaac tgaaagaaca
     3121 gattttggtg actgcgctcc tccaagccag ttaccttggt tcaaagagtt ggtagctcag
     3181 cgaaccttga gaaaaccacc gttggtagcg gtggtttttc tttatttatg agatgatgaa
     3241 tcaatcggtc tatcaagtca acgaacagct attccgttga caaaaaaaca agatctgtac
     3301 atattc
//
LOCUS       DAZFDC010000254         3288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFDC010000254 DAZFDC010000000
VERSION     DAZFDC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.256"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..692
                     /locus_tag="ACHJ48_004826"
     CDS             <1..692
                     /locus_tag="ACHJ48_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO4710185.1"
                     /translation="NVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAV
                     RRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQIG
                     GKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTL
                     LFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAK
                     RIARLAEQSTD"
     gene            791..1804
                     /gene="ybjS"
                     /locus_tag="ACHJ48_004827"
     CDS             791..1804
                     /gene="ybjS"
                     /locus_tag="ACHJ48_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO4710186.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1801..2631)
                     /gene="amiD"
                     /locus_tag="ACHJ48_004828"
     CDS             complement(1801..2631)
                     /gene="amiD"
                     /locus_tag="ACHJ48_004828"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO4710187.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2628..2951)
                     /gene="ybjQ"
                     /locus_tag="ACHJ48_004829"
     CDS             complement(2628..2951)
                     /gene="ybjQ"
                     /locus_tag="ACHJ48_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO4710188.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3162..>3288)
                     /locus_tag="ACHJ48_004830"
     CDS             complement(3162..>3288)
                     /locus_tag="ACHJ48_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710189.1"
                     /translation="ILLLSCFAAADQKAIQRYIPQAQDGEDFRNHLQYKLTLPTH"
BASE COUNT          763 a          898 c          844 g          783 t
ORIGIN      
        1 tcaatgtgat tacttccgta ccgcccacca ccgccagggc gttgattcag gggctgaaac
       61 acgatctgct ggcggatgac accgcgctac gtgcgctcat cccacaacgg ctgatcgctt
      121 tcgatgacgc ggtacgtcgc accctgaaag aagaagaaaa gctggtcaac tccagcgact
      181 ggggatacga cactcaggcc tttgcccgct ggcgaccaga gtatggttat tttgccaaac
      241 aggcgggatt taccgttaaa acgtccgcca gccttgcggc tttatggcag gtggtgaacc
      301 aaatcggcgg taaagagcgt tatttctttg gcaatatttt gtggcagaca cgggcgttga
      361 tggaccgtgc gatcggtcat aaattagcga aaggccgtcc ggagcgcgaa tatttgcaaa
      421 ctggcgatgc ggtggatagc tggaaagtga ttgtcgttga accggaaaaa caacttacgt
      481 tgttatttgg catgaaagca ccggggctgg gacgactgtg ttttaccctg gaagataaag
      541 gcgactatcg tactatcgat gtccgcgctt tctggcatcc gcacggtatg ccggggctgt
      601 tttactggtt attgatgatc cccgcgcatc tgtttatttt tcgcggaatg gcaaaacgaa
      661 tcgccagact ggcagaacaa agcacagatt aatcgacaaa acctgcgtta ttctttcatg
      721 tttcccattg catcaagggg gaaatcacgg aagaatgcgc acgtcatttt ctcaacaaca
      781 gtggattgat atgaaggtac tggttaccgg cgccaccagc ggcttaggtc gaaacgcggt
      841 agagttttta tgccagaaag gcatcagcgt gcgagcgacc ggtcgcaacg aggcaatggg
      901 taaattgctg gaaaaaatgg gcgcagagtt tgttccggcg gatctgaccg agctggtctc
      961 atcacaagct aaagtgatgc tcgcgggcat tgatacgctg tggcactgct ccagctttac
     1021 ctctccctgg gggacacaac aggctttcga tctggctaac gttcgcgcca ctcgccgcct
     1081 aggtgaatgg gctgtcgcct ggggtgtacg taactttatt catatctctt ctccctccct
     1141 gtacttcgat tatcaccacc atcgcgatat taaagaagat tttcgccctc accgcttcgc
     1201 caacgagttt gcccgcagca aagcggccag cgaagaagtg atcaatatgc tttcgcaggc
     1261 gaatccacaa acgcgcttta ctattctgcg cccacaaagt ctgttcggac cgcacgataa
     1321 agtctttatc ccccgtcttg cgcatatgat gcaccactac ggcagcattc tgttaccgca
     1381 tggcggcagt gcgctggtgg atatgaccta ctatgaaaat gccgtgcacg ccatgtggct
     1441 ggcaagccag gaagcctgcg ataagctacc ttccgggcgt gtgtacaaca tcaccaacgg
     1501 cgagcatcgc acactgcgca gcatcgtgca gaagctgatc gacgagttga atattgactg
     1561 tcgtattcgt tccgtccctt acccgatgct ggatatgatc gcccgcagca tggagcgttt
     1621 aggccgcaag tcagcaaaag agccgccgct gacccactac ggcgtctcga agcttaattt
     1681 tgactttacg ctggatatta cgcgggcaca ggaagagtta ggttatcagc cggtcatcac
     1741 cctggatgaa ggtatcgaga aaactgccgc ctggctgcgc gaccacggaa aactgccgcg
     1801 ctaatcctgc ccgtatttct ccagcaacgc ttcggcaatc gcctgagttt ctgcatccgc
     1861 ttcgccgtta tataacgtcg ggcggaaatg catctggaac gccataatca cgcgccgctg
     1921 ctcgcgcggt gtcatatcag gtttaacgtc ataaccgtag cgcgccaaaa gctccagcaa
     1981 tgacgcggta tctaccggtg tgtgcggcgc gcgcccggca aggtaaaagt tcacccgctg
     2041 cgcatccggc caggcaccaa tcccctgctg cgccagttgc tgccagggaa ataatggccc
     2101 cggatcgtct ttgcgctgcg gggcgatatc cgcatgtgcc actacgtttt ccggcttgat
     2161 gtgataacgg gcaataatat ctttcgccag cggaataagc gcctgaatct gtgccggttc
     2221 aaacggggca aaatatttca cccccgccga tttttgccag ccacggtttt ccagctcaat
     2281 gccaatagag gtgtcgttaa ggcgcgttgc cccgcgccag gcgctaatcc ccgcatgcca
     2341 ggccagttct tgttccggca ccaattgcca gatgcgcggt ttaccgttgt atcgcggtgg
     2401 taccgcaggg accagataat gcgagctgac ctgcttatcg gtcagtgtcg ccagcgagct
     2461 atcaaaatca tctgcggtgt agtggatcac cagcacttta atgcgcggat acgccgcctg
     2521 cgcctggcgt cgggtatcaa gctgatatcc ctctttctcg acaatgcctt tttcgcctgc
     2581 gcaccctgcc agtaacagag cgacagcgat cagccagaag actcttctca tcgacgcgtt
     2641 ttcaccgccg taccgctgac gctaaccatc agcatactgc cgttttgccc gaccgtttcg
     2701 tagtcgatat caataccgac tacggcatcg gcccccagcg cccgcgcctg ggagccaagc
     2761 tcctcaaagg cgatctcccg cgctttacgt agctcttttt cgtaggcacc ggagcgtccg
     2821 ccaacgatat cgcgaatacc agcaaagaaa tcacggaaaa tattcgcgcc taaaatcgct
     2881 tcgccagtca ccacaccgca atattcaacg atggtctggc cttccagagt tggagttgtg
     2941 gaaaattgca tgatcgatct ccttgtttgt taagccttcg atctctaaag cattatcgga
     3001 ctgatatgcc atgttgggaa gaaggttttg atcagtttag gaaatcatct cattgtgtca
     3061 aaaacagcta cataagcctg tacaattatc tgtttcgtaa ccggatattg gtttcattat
     3121 ggagcaccgc ttacctgccc tattatgttg tgccggcaac attaatgagt gggaagagtc
     3181 aacttatatt gtaaatgatt cctaaagtct tccccatctt gcgcctgagg tatataccgc
     3241 tggattgctt tttggtccgc cgccgcaaaa caagaaagta ataagatc
//
LOCUS       DAZFDC010000255         3255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFDC010000255 DAZFDC010000000
VERSION     DAZFDC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.257"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1044..2189
                     /gene="espG"
                     /locus_tag="ACHJ48_004831"
     CDS             1044..2189
                     /gene="espG"
                     /locus_tag="ACHJ48_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFO4710190.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLFKVLSFG
                     LWNPTYSRSERQSFQELLTVLEPVYPLPNELGRVSARFSDGSSLRISVTNSELVEAEI
                     RTANNEKITVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEMDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQKLLRDALG
                     LKDTHSSPTRNVIDHGISRHDAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCIIDEMVSALQPRYAASETYLQNT"
     gene            2317..3135
                     /locus_tag="ACHJ48_004832"
     CDS             2317..3135
                     /locus_tag="ACHJ48_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO4710191.1"
                     /translation="MKRKPLIAFFIAIFIALTILILFPFNCTYKKSPQEHSLSFIKQL
                     PNTVNLSSLTYNKEDDFLYATQNSPAQLLKITKSGDIMDRAPLPFISDAETIEHIQGN
                     IFAAVDEKTSELFFFTVTKDMHISFRNKIQLEKFNKKNRGFEGLAWKADDRMLFVAKE
                     RRPSKIFIYQLSPDLLSVKQATIPEALNDIRVNDISGLAFNNESLMILSDESRKLLKF
                     NLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLPDESIYVASEPDILAKFVPNK"
BASE COUNT         1111 a          653 c          561 g          930 t
ORIGIN      
        1 aaacgacttt aataattgca tttccattta gttaagcgtt tctctttata agatagatct
       61 cattgcataa acaaataaaa cagaataaaa accaagctga accgtttcac tatactctac
      121 ttaattcgag ttacaggaag gcggcaagcg agtgaatccc caggagctta cataagtaag
      181 tgattggggt aagcgaacgc agtcgcagca catgcaactt gaagtatgac gagtatatat
      241 atctattagc tgtttcccgg acataagcta cagccagcac taacctggaa cgggtgcatt
      301 ataaaaagga acaagggaca aatagctgaa cagtaaccgc gttagtctct tcccgcataa
      361 ccgtaccatt cgtattaaga agtattgccc atatccattc ggcgaaagag gatctgccat
      421 acatcaagtc cgttttactt ggcatcagat catcgccgtc attaactctg ttaaagtcgg
      481 acaaacgatt agcaagatct gtgattcaaa agtgcttagc gctaaggtgg gactggttac
      541 agattcatta accctgttct ttgtcgcacc tatcgatcgg tgtaatgcca caacaggttt
      601 atgttaatca gaacaatgcg cccctctata aagagaattc tttgataagg caacttgaaa
      661 agggaaagct aactgatatc aatgtaccac tttagtaatc atcacattat atacaggcca
      721 aaataagttt cctgaaagcc agtatctcta aatcaatcat taatgatata ttttatcgtc
      781 acaataaaaa attatctaaa ttctattact tatgtaaata agtacaacct tttcatagac
      841 acaaaaacta tggctgacgc atcacttgca atcaattatc catcattaga ttaatatttt
      901 cacttatttt tcagaaggta aaaataactt tctatgcgtt taaaacaccc gctataaata
      961 tcttgtatat tattgacact atatgatgct atatgatact tgttgccaaa ttgttcatta
     1021 caaaccagat aggagaatct ctcatgataa atggacttaa taatgactcc gcatctttag
     1081 ttttagatgc tgcaatgaaa gttaattctg ggtttaaaaa aagctgggat gagatgtcat
     1141 gcgctgaaaa gttatttaaa gtacttagtt ttggtttatg gaatccaacg tacagtcgta
     1201 gtgaaagaca atcatttcaa gagttgttaa ccgttttaga gcctgtatat ccacttccca
     1261 atgaattagg cagagtatct gctcgttttt cagatggttc atccttaaga atttccgtca
     1321 ctaacagcga acttgttgaa gccgagattc gcacagcaaa taatgaaaag attactgtgc
     1381 tcctggagtc aaacgaacaa aataggttat tacaatcttt acccatcgat cgccacatgc
     1441 catacattca ggttcatcgt gccttatctg agatggacct gactgatact acctcaatgc
     1501 gcaatctact tggttttacg tcaaaactat caacaacctt gattcctcat aatgctcaaa
     1561 cagatccgct ttccgggcct acaccattca gctctatctt tatggataca tgtcgaggac
     1621 tcggcaatgc aaagctttca ctcaatggtg ttgatatacc tgcaaatgca caaaaattgc
     1681 ttcgcgatgc actaggactt aaagacacac attcatcacc aacccggaat gttatagatc
     1741 atggtatttc tcgccatgat gcagagcaaa tagcaagaga aagcagcggc agtgataaac
     1801 agaaagctga agttgtggaa tttttatgcc atccagaagc agcaacggcc atatgctcgg
     1861 ctttctatca atctttcaat gtgccagcct taacgttgac acatgaaagg atctctaaag
     1921 ccagtgaata caatgcggaa agatcattag atacacctaa cgcttgcatt aacatcagta
     1981 tctctcaatc atcagatgga aacatttatg ttaccagcca tactggggtt ctgataatgg
     2041 cgccagaaga ccgccccaac gagatgggca tgttgacgaa caggacttct tatgaagtgc
     2101 cgcaaggtgt gaaatgtata atcgatgaaa tggtaagtgc gctacaacca aggtatgccg
     2161 catctgaaac gtacttacaa aacacttaaa ataaaacttt attaatcaaa actaataata
     2221 gaaaccacac ccataattat taacaattta ttccgaagtt aatcacaatt aaacaaagat
     2281 atttattctg caaacaattt acagcaaata gagaaaatga aaagaaaacc attgattgca
     2341 tttttcatcg cgatatttat tgcactaacc atattaatat tatttccatt taattgtact
     2401 tacaaaaaat cacctcaaga acactcactt tcatttataa aacaattacc aaatacggtg
     2461 aacttatcat ctcttactta taataaagag gatgatttcc tgtatgccac ccagaatagc
     2521 cctgcacagc tacttaaaat aaccaaaagt ggcgacatca tggacagagc cccactgcct
     2581 tttatctctg atgccgaaac aattgaacac atacaaggga acatttttgc tgcagtagac
     2641 gaaaagactt ctgaactatt cttttttaca gttacaaaag atatgcatat ctcctttcga
     2701 aataaaattc agttagaaaa attcaataaa aagaatagag ggtttgaagg gctggcatgg
     2761 aaagcagatg atcggatgct gtttgtcgca aaagaaagac gcccaagtaa aatatttata
     2821 tatcaacttt ctccagacct gctgagtgta aagcaagcaa ctattccaga ggcactaaat
     2881 gatatacgtg ttaacgatat atccggcctg gcctttaata atgaaagtct gatgattctg
     2941 tccgatgaat cgcgtaaatt actcaagttt aacctgacag agatgtcttt cattgagatg
     3001 cttgacctga caaaaggaaa tcattctctg accagtgatt tgccccaacc tgaaggaatt
     3061 gtaactctgc ctgatgaaag catttatgtt gccagtgaac ctgatatcct cgcgaaattt
     3121 gtccctaata agtaagtctg tcactggcct tattatttat cgaccaaacc tggcaatctc
     3181 ataaagttat aaaaatacga gtataatttc acgctaaata aacacattaa tagttgaaaa
     3241 ggatatcagt tatta
//
LOCUS       DAZFDC010000256         3219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFDC010000256 DAZFDC010000000
VERSION     DAZFDC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.258"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..652
                     /locus_tag="ACHJ48_004833"
     CDS             <1..652
                     /locus_tag="ACHJ48_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO4710192.1"
                     /translation="KILVNKNRNYEILYGRDHVIYINTNSLDEAVWVKQALEKNQPGK
                     PVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNPRLTVSKQRNAITQQLIDNLI
                     KGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTLSDS
                     ELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSKWTFQE"
     gene            666..905
                     /locus_tag="ACHJ48_004834"
     CDS             666..905
                     /locus_tag="ACHJ48_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFO4710193.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            925..1257
                     /gene="sctI"
                     /locus_tag="ACHJ48_004835"
     CDS             925..1257
                     /gene="sctI"
                     /locus_tag="ACHJ48_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO4710194.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALTSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            1254..1988
                     /locus_tag="ACHJ48_004836"
     CDS             1254..1988
                     /locus_tag="ACHJ48_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO4710195.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            2004..2585
                     /locus_tag="ACHJ48_004837"
     CDS             2004..2585
                     /locus_tag="ACHJ48_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO4710196.1"
                     /translation="MNLALRKIIYAPISYIHPQRVSLNNTPINNPVLRSITNEMILLQ
                     YNLSVEHFNLNSSLIYYINNWNLLPLICLLSGCHFYRERFAERGFFYKVPDVLRDYLS
                     AIPLEINEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHILS
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            2578..2787
                     /locus_tag="ACHJ48_004838"
     CDS             2578..2787
                     /locus_tag="ACHJ48_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710197.1"
                     /translation="MIKRKISDGLKEIVSLKEKILLETTAKIQSIEEKREEKFIQGYY
                     DGYTKGIIDVMDNFIPLISLAMLRT"
     gene            2804..>3219
                     /locus_tag="ACHJ48_004839"
     CDS             2804..>3219
                     /locus_tag="ACHJ48_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710198.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWVTKLPSISGPVNLHIPTS
                     FKDKSLEVESYFVDKSIWNVHIAYHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVNEICEQARHYLVEKMCETHSLAMNNSVLT"
BASE COUNT         1111 a          519 c          598 g          991 t
ORIGIN      
        1 taagattctt gtaaacaaaa acaggaatta tgaaatttta tatggtagag atcatgtcat
       61 ctatatcaat accaatagtt tggatgaagc agtctgggtc aaacaagcac tggaaaaaaa
      121 tcaaccagga aagccagtac gggtgataaa tcctgatgat gaatcgatac gaattttttc
      181 atggcttgct gataatttcc ctgatttaca atattttaaa cttcagctat tagatgccag
      241 taatcccaga ctgaccgtga gtaagcaacg gaatgctatc acacagcaac taatcgacaa
      301 tcttattaaa gggttactac aaactatgcc atatgccagc aatataagta ttgcggtatt
      361 agatgataat gtattggaaa gtcaggctat tgaaacattg tctgcaatag gtctttctta
      421 tgaaaaatat aaaacagcta acaatgtgta tttcaatatc attggtacgt taagcgacag
      481 tgaattaaac aaaataaata actatgttga cgaatattat aaacaatggg gtaaacaata
      541 tgtaagattt aatgtgaatt tgaaaaatca ggacacaaat aatagttcat ttagctacgg
      601 agataaccga ttcgagaagt ctcaaggtag caagtggaca tttcaggaat aattgaggag
      661 gacttatggc agattggaat ggttatatta tggacatcag caaacaattt gatcagggcg
      721 ttgatgatct gaaccagcaa gttgaaaaag cgttggagga tttagcaacc aatccttccg
      781 acccgaaatt ccttgccgaa tatcaaagtg cattagctga gtatacatta tatcgaaatg
      841 cgcaatctaa cgttgtaaaa gcgtataaag atcttgattc ggcaatcata caaaacttcc
      901 gctaaactgg ataaggattt tattatgtca gtaagcaata tgccacctat tgatagagct
      961 gagcaatcaa ctgctcatga aatacagcaa gcgaaagtga ttgatttaaa tgaccgggtt
     1021 ctcaatcttg acaacccgga tgataaaatg atctccgctt ttgctaacta tgctgtacaa
     1081 actgaaaatt ggcagcaaaa tgcgttacaa gcattaacgt ctgataaaaa aggattaact
     1141 cctgaaaaat tattagtctt gcaggatcat gtgcttaatt ataatgtcga ggtttcattg
     1201 gtgggaacat tagcaagaaa aattgtcgct gcagttgaga cgttgacccg ctcatgaaat
     1261 atatatcatt attattgttt atcctgctgt tgtgcggctg caagcagcag gaattactga
     1321 accatcttga tcaacagcaa gctaatgatg tccttgctgt tttacaaaga cataatatta
     1381 atgctgaaaa gaaggatcaa ggcaaaactg gattctcaat ctatgttgaa ccaacggatt
     1441 ttgcttctgc ggtggattgg ctaaaaatct acaatctccc gggtaagcct gatattcaaa
     1501 tatcgcagat gttccctgca gatgcactcg tttcatctcc acgagcggaa aaagcaaggc
     1561 tatattctgc tattgagcag cggttagagc aatctctcaa aattatggat ggtattgttt
     1621 cttcgagagt acatgttagt tacgatgttg atactggcga tagtggtaaa actgctttac
     1681 caattcatat ttctgtactt gcagtatatg aaaaagatat caatccagaa ataaaaataa
     1741 atgacataaa gagatttatt gttaatagtt ttgcaagcgt acaatatgag aatatttctg
     1801 ttgtattgtc aaaacgaaga gacattattg agcaagcacc aacatatgaa attagcgaac
     1861 ctgtttttgc ttatgataag gctatgccgg tttctatctt acttgcatta atatccgtag
     1921 cgacttgctg gctgttatgg aagtataggg ccattctaac taatttagtc aggttgaaaa
     1981 ttaagtagac atatgagaac gttatgaatc ttgccctaag aaagattata tatgctccaa
     2041 ttagttatat tcatcctcaa cgagtaagct tgaataatac acctattaac aatccggtgc
     2101 tgagatctat taccaatgaa atgattctcc tgcagtataa tctttcagtt gaacatttta
     2161 atctgaatag ttcacttatt tactacataa acaattggaa tttacttcca ttgatctgtt
     2221 tactcagtgg ttgtcacttt tatcgagaaa ggtttgcaga acgcgggttt ttttataagg
     2281 ttcctgatgt actaagggat tatttatcag ctataccgct ggaaataaat gaaaaggcac
     2341 gatacaaacc cggcatcgct aactatcaca atatcatcac atgtggtttt tcaacgttgt
     2401 taccctatat aaggcaacaa ccattggcaa tgcagcaacg atttaattta ttatttcctg
     2461 attttgttga ccacatacta tcgccgttac cacttgcatc aacactttta gagagaatta
     2521 cattttatgc gaaaaaaaat agagatgagc ttgataaaat ctcctgcaaa tggtgttgtg
     2581 attaaaagaa aaatttctga tggattgaaa gaaatagttt ctcttaaaga aaaaatcctt
     2641 cttgaaacca ccgcaaaaat tcagagtatt gaggaaaaga gagaagaaaa atttattcaa
     2701 ggttattatg atggctatac aaaaggcatt attgatgtga tggataattt tatacccttg
     2761 ataagcttag ctatgctcag aacttgaaaa aaaaagaata aacatgataa acgatctgaa
     2821 gagtatattg ttaaaatctt cagaagaagt tgatgttttt atcaaaattt ttgaaagttg
     2881 ggtaacaaaa ttaccatcaa tatcaggtcc tgtaaatctc catataccta ccagtttcaa
     2941 agataagtct cttgaagtag agtcatattt tgtcgacaag tcaatatgga atgtccatat
     3001 tgcatatcat gatgataagc gttttgtgtt ttttaccgat caatttattg ctgaatttag
     3061 tccgcaggaa tttgtggata attgtgagca atatttaatt aataatcatt gtttctcccc
     3121 tgacaaggtt aacgaaatct gcgaacaagc caggcattat ttagttgaaa aaatgtgtga
     3181 aacacattct ttggctatga ataattctgt tttgacaaa
//
LOCUS       DAZFDC010000257         3212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFDC010000257 DAZFDC010000000
VERSION     DAZFDC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.259"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..495)
                     /locus_tag="ACHJ48_004840"
     CDS             complement(<1..495)
                     /locus_tag="ACHJ48_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFO4710199.1"
                     /translation="MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTT
                     LLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHA
                     LKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
                     IERGE"
     gene            complement(477..986)
                     /locus_tag="ACHJ48_004841"
     CDS             complement(477..986)
                     /locus_tag="ACHJ48_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO4710200.1"
                     /translation="MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGY
                     WFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHY
                     IVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
                     KDIICLMRQ"
     gene            complement(989..1954)
                     /locus_tag="ACHJ48_004842"
     CDS             complement(989..1954)
                     /locus_tag="ACHJ48_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFO4710201.1"
                     /translation="MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDS
                     SAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKL
                     LFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
                     VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMA
                     SASIYVMEMPYDIWQKNTKVMLSHINIGTGIDCTICELAETIAKVVGYKGHITFDTTK
                     PDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYRG"
     gene            complement(1957..3075)
                     /gene="gmd"
                     /locus_tag="ACHJ48_004843"
     CDS             complement(1957..3075)
                     /gene="gmd"
                     /locus_tag="ACHJ48_004843"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO4710202.1"
                     /translation="MTKVALITGVTGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERI
                     DHIYQDPHGSNPNFHLHYGDLTDSSNLTRILKEVQPDEVYNLAAMSHVAVSFESPEYT
                     ADVDAIGTLRLLEAIRFLGLENKTRFYQASTSELYGLVQEIPQKESTPFYPRSPYAVA
                     KLYAYWITVNYRESYGIYACNGILFNHESPRRGETFVTRKITRGLANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVRMQWLMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKMSF
                     VGKGIEEKGIVDSVEGQDAPGVKPGDVIVAVDPRYFRPAEVDTLLGDPSKANLKLGWR
                     PEITLAEMISEMVAKDLEAAKKHSLLKSHGFSVSLALE"
     gene            complement(3095..>3212)
                     /locus_tag="ACHJ48_004844"
     CDS             complement(3095..>3212)
                     /locus_tag="ACHJ48_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HFO4710203.1"
                     /translation="ESKIFYEPTTLIELGLKRRNACADFLLDVVKQEIESRS"
BASE COUNT          985 a          669 c          570 g          988 t
ORIGIN      
        1 ttcacctctc tcaatatacc cataaccagt ttcagcatat tctggtataa ttccaaacgt
       61 tacaatttta ccttgattag cgatgggagt tgcattttta atagcatcac agaaaacact
      121 ttctttagct ataacgtggt ctgccgcaag aactagaagc aatggatctt cctgaggatt
      181 acgttttaac gcatgaaacg cagatatcgc tattgctggt gcagtatttc gcccgcatgg
      241 ttctagaata atattaccat ttaatttatt tatttccctt aactgttcag ccacaacaaa
      301 gcgatgctgt tcatttgtta tcactaatgg tttttgacat gataggcctg aaagtcgtag
      361 caaagtcgtt tgtaacaagg tgttatcacc agagagttgt aaaaactgct ttggatatag
      421 ttcacgagaa agtggccaaa gacgactgcc tgtaccaccg gccataacta cagcaattat
      481 tggcgcatca gacatattat atccttatta tctaagccaa tcgcatcatt tgttttattg
      541 ttaaaatatg cgcgacaatt ataatgcaca tcgtcatcat ttatcagctt tgctcgcgat
      601 agccagcaat aagcattatg ttgtgacttc ggtaatttca aaatgtttgg ttgtaattta
      661 agaaggaatg ctataactat ataatgcgtt gaaaagtctc cccccatact attgtcttcg
      721 tagaagtgct gccagatacc ataaaactta cccacagaga gaggcaaacg aattcctagt
      781 tcaatttctg tcaatcgttc aaaggctgtc tgcaattttt catctttcaa caccctacca
      841 ccaggaacga accaatagcc ctgtgccggg cggttgattc gttttcctag caaaatttcg
      901 ccaaactcgt tttccacaat caaatctata gaaataagag gagtagacct tacaattgtg
      961 gcaaagtctt gggaatgtaa aaacattatt acccccgata ttgaagttgg ttttcaagaa
     1021 accagttgta tgtattttca agacccttgt gaagggtaat tttatgattc caacctagtt
     1081 gatgaagaag cgttacatca agtagttttc gaggggctcc atcgggcttt gttgtatcga
     1141 acgtaatatg ccctttataa cctacaactt ttgctattgt ttccgcaagc tcacaaatcg
     1201 tgcagtcaat acctgttcca atattgatat gagacaacat tactttagta tttttttgcc
     1261 atatatcgta tggcatctcc atgacataaa tgcttgcaga agccatatca tctacatgta
     1321 agaattcacg ctttggagta ccacttcccc aaacaacaac attcggagaa ttgttttcca
     1381 cagcatcatg aaagcggcgc aaaagcgccg gaatcacatg agaattactt ggatgaaaat
     1441 tgtcatttgg accataaaga ttggttggca ttactgaacg gtaatcacgc ccaaactgac
     1501 ggttataaga ttcacataat ttaatacctg caatttttgc gatagcataa ggttcatttg
     1561 ttggctcaag tttcccttgt aataattcgt cttccataat cggttggtgt gctaacttag
     1621 gataaataca cgacgaaccg aggaaaagca gtttatttac attatttttg tgggcagcat
     1681 gaatgacatt cgcctctatc attatattct catatataaa atcggcagga taagaactgt
     1741 tagctaaaat acctccgact tttgctgctg ccaaataaac ctggtcgatt ttctgtgaag
     1801 aaaaaaaatc caaaacagcg ctactatcca acaagttcaa ttcatcccga gtacgtaaaa
     1861 ccaactcaac atcatcacgt tgtttgaggc gtcgggtaat agctgatcca accattcctt
     1921 ggtgaccagc aataaaaata cgttgcttat tcatcatcat tccagagcta agcttacaga
     1981 aaaaccatgc gattttaaaa gagaatgttt tttagcggct tcaagatctt tggcaaccat
     2041 ttcagaaatc atttcagcaa gagtaatttc tggtctccaa ccaagtttga gattagcttt
     2101 gctcggatct ccaagcaaag tatcaacttc agctggtcgg aaataacgag gatcaacagc
     2161 aacaatgaca tcacctggtt tcacacctgg agcatcctgt ccttcaaccg aatctacaat
     2221 gcctttttct tcgattcctt taccaacaaa gctcatctta ataccaagtt gtgctgctgc
     2281 catttcgaca aactgacgga ctgagtattg gactcctgtt gcaatcacaa aatcttcggg
     2341 ttgctcctgt tgtaacatca accattgcat tctaacataa tcttttgcat gtccccaatc
     2401 tcgtaacgaa tccatattcc ctaaatacaa acatgattcc aagccttgtg caatatttgc
     2461 aagtcctcga gtaattttcc ttgttacaaa cgtttctcca cggcgtggag attcatgatt
     2521 gaacaatata ccattacatg cataaatacc atatgactct cgataattta ccgtgatcca
     2581 atatgcgtaa agttttgcaa ctgcataagg ggaacgagga taaaaagggg tggattcttt
     2641 ttgagggatt tcctgaacaa gtccatataa ttctgaggtt gaagcttgat agaaacgcgt
     2701 tttgttttcc aatcctaaaa agcgaattgc ttccagtaaa cgtaatgtac caattgcatc
     2761 gacatcggct gtatattctg gagactcaaa agaaactgct acgtgactca tagcagctaa
     2821 attatatact tcatctggct gtacctcctt tagaattcta gtgaggttag atgaatcagt
     2881 cagatctcca tagtgcaagt gaaaatttgg gttagaacca tgtggatctt gataaatatg
     2941 gtctatgcgt tctgtattaa aagatgaggc tcggcgtttg ataccatgaa cttcataccc
     3001 tttatcaagc aaaaactcag ctagataaga tccatcttgt ccagttacac ctgtaataag
     3061 agcgacttta gtcattatat acctcttgaa tatattaaga tcgggattca atttcttgtt
     3121 tcacaacatc taaaagaaaa tctgcacacg cattgcgtct tttgagaccc aattctatca
     3181 gagttgtagg ttcataaaaa attttagatt ca
//
LOCUS       DAZFDC010000258         3207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFDC010000258 DAZFDC010000000
VERSION     DAZFDC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.260"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            191..649
                     /locus_tag="ACHJ48_004845"
     CDS             191..649
                     /locus_tag="ACHJ48_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710204.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            887..1584
                     /locus_tag="ACHJ48_004846"
     CDS             join(887..1136,1136..1584)
                     /locus_tag="ACHJ48_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
                     /protein_id="HFO4710205.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGKHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            1601..1861
                     /locus_tag="ACHJ48_004847"
                     /pseudo
     CDS             1601..1861
                     /locus_tag="ACHJ48_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(2003..3127)
                     /locus_tag="ACHJ48_004848"
     CDS             complement(2003..3127)
                     /locus_tag="ACHJ48_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO4710206.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          942 a          693 c          641 g          931 t
ORIGIN      
        1 cattaaatca atcacctgat taaaccatga aagtgtgatg taagtcaaaa ttactgatac
       61 tatcaattga taatgattat ctctatcatt atcgtgaaat tcgagaatga aaggagaagt
      121 agtcattata agccttttat aaatcgtcat catcatagta aagtagaaca ctttttatat
      181 gagttactat atgaaaatat actggacaag aaaacgtatt cctgaacttt cagcacttcc
      241 accttcatta agaaagaaaa attttactga cgcatataat gcagcatctt ctcatatcga
      301 atactggata ggtgctggcg tctcatttat ctcgatgatg attttatcca gagtatatga
      361 ctttctttta cccgcccaag atactttccc gggtgatatc atcagaagtt tatgtgttgt
      421 gtgtccatcc atattaatct ggtttcagtt ttcggtatat gtcatgagga aatattacag
      481 acatattctg gtgcgaggaa aagaaaccga aaccatttca gaaaggttga tacgtgaggc
      541 tgatacacga gaatatgaac tatggcgtcc ggtccgtcgc tttttctcca ttgttttcct
      601 cattgttcta ttaggttgca ttcattcact gataacaact ataaaataaa agttatggct
      661 gttctgatac aggaaaataa acgttcattc tatggttacc tcttcctgtt atatagaaga
      721 attttaaacg acaagtaagg gaatataaat gaaaataaca aaaaataaaa atgcaactat
      781 aaaaaacagt catcagtcgg ctgcttttca atgtttacct tatcattttc tggtaatgct
      841 gccaacttac tgatttagtg tatgatggtg attttaaggt gcttgcgtgg cttccatttc
      901 catcagatgt ccttcctgct ccgctactga aggcgtggtg cgtaacggca aaagcactgc
      961 cggacatcag cgctatctct gctctccttg ccgtaaaaca tggcaactac agttcactta
     1021 caccgcctct cagcccggta agcaccagaa aatcattgat atggccatga atggcgtcgg
     1081 atgtcgcgcc agtgcacgca ttatgggcgt tggcctcaac acggttttac gtcacttaaa
     1141 aaactcaggc cgcagtcggt aacctcgcgc atacaaccgg gcagtgatgt gattgtctgc
     1201 gctgaaatgg acgaacagtg gggctacgtc ggtgctaaat cacgtcagcg ctggctgttt
     1261 tacgcgtatg acaggatacg gaggacggtt gtggcgcacg tcttcggtga acgcactctg
     1321 gccacactgg agcgtcttct gagcctgctg tcggcctttg aggtcgtggt atggatgacg
     1381 gatggctggc cgctgtatga atcacgcctg aagggaaagc tgcacgttat cagcaagcgt
     1441 tacactcagc gcattgagcg acataatctg aatctgagac aacatctggc aaggctggga
     1501 cggaagtcac tgtcgttctc aaaatcggtg gagctgcatg acaaggtcat cgggcattat
     1561 ctgaacataa aacactatca gtaagttgga gtcattacct gagaataata cctcctgggt
     1621 aagtgagcag atactttcag cgcctccgaa agatgtaaat attcgacttg gagtcggttc
     1681 tttagaaggt acaaccgtct cacatgttca gcgattgcat cattcgttaa tcgcagctgg
     1741 tttggaaagt aacctcactg tctataccgg tggtcacgat tatgcctggt ggcgcggagc
     1801 aattattgat gaattagcaa attataattg caggaaggta tcaaataata actctgtgta
     1861 aaatatcgtt ttatcgtcag ataactcttt ctgcaatatt attatgggag actatctcat
     1921 ttatttagtg atgtccttca tttatgtgat gaaggacatt atattactca taacatttct
     1981 cgttgaaaat ttcagcaata cgctacaatt gtttcccgtt gttttttaat tttctcatca
     2041 aaacctctgc aatctctgca ggactgggtt gttcagccat ttcagcggct acctgatctg
     2101 aacagaagcg tagtgaatca tcataaagaa ggcgatttac caaatcactg gtcagtccat
     2161 gcttgtccgg aataatccca cacccacgct tcgcaacaat ttctgcatta acagagcgat
     2221 ctgcaccttc gccaaacaca atctgtggta ttccactata cagcgctgtc agggtattac
     2281 ctgcgccacc gtgatgaata aatccatcag ctccattaag gaatacaccc ataggtatcc
     2341 agtcaaccag acgtacattt gatggtagtt ttcgtaaacc agtacgagca tttattgcaa
     2401 gttgcaaaat gatatcagca tcaacttcat ttgcagaatc cataacccat gaaatcagct
     2461 ccagaccatc aaccattggt tttacagtgc caaggctgat aagtaaacgt ttccgatcag
     2521 aatccctgtc ccaccatgtt tcctttacag cacctccgtt ataaggaata tatctcattg
     2581 agataaccgg ttctccagca tttttaagaa tgctcatact tgggggagca acatcaatcc
     2641 atgctaaatc acatagtgga ccgctgactc catgccgcct gtaagcattg cttaaagaac
     2701 ggtttaacat ctgaatatgg gcagatgtat gcgcgaatcc aacagccagc atcactgaag
     2761 gaattctata tttagcagca accaatgggc ctgccggatc aagaggggga tagactatta
     2821 aatctggcct ccattttcct gcaaaatcga tgaggttatc tgccatttca tcgctaaaaa
     2881 aagagaaatg accaataata ttactttttt tcctcaactc ttcctgatgg cgatatccag
     2941 cctctgaatc taaacctgga actgcatcaa aaacaaccag tccagcttct gctgctttat
     3001 tcgcgaattt gccagcgcta ctaattacta catcatgtcc aattacacga aaggcctgag
     3061 ccagagaaat caatgggtat aacaaaccgt acagcggtgg gccaataaaa agaatacgtt
     3121 ttcgcattaa aaaagtccgt cttctgctta cctgtttatt tttctatctc taagaaaaca
     3181 taattaatat tatttttatg tgagctt
//
LOCUS       DAZFDC010000259         3203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFDC010000259 DAZFDC010000000
VERSION     DAZFDC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.261"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..1053
                     /locus_tag="ACHJ48_004849"
     CDS             10..1053
                     /locus_tag="ACHJ48_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO4710207.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDKD
                     TDTELNPTHNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQR
                     AAVWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKG
                     GSHAASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAHGGGVFEPLDSEERGR
                     TFEALDCPDWCDD"
     gene            1046..1507
                     /locus_tag="ACHJ48_004850"
     CDS             1046..1507
                     /locus_tag="ACHJ48_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO4710208.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            1541..2257
                     /locus_tag="ACHJ48_004851"
     CDS             1541..2257
                     /locus_tag="ACHJ48_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO4710209.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQTGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASITETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            2290..2571
                     /locus_tag="ACHJ48_004852"
     CDS             2290..2571
                     /locus_tag="ACHJ48_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4710210.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2568..2795
                     /locus_tag="ACHJ48_004853"
     CDS             2568..2795
                     /locus_tag="ACHJ48_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710211.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2788..3099
                     /locus_tag="ACHJ48_004854"
     CDS             2788..3099
                     /locus_tag="ACHJ48_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710212.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
BASE COUNT          862 a          691 c          990 g          660 t
ORIGIN      
        1 gaggtgagta tggctaatgc ctggctcaga ttgtggcatg acatgccaaa tgaccccaag
       61 tggcgaacga ttgccagggt atcaggacag ccaatcgcaa cagtgatggc agtgtatatc
      121 catcttctgg tgagcgcgtc acgaaatgtc acgacatgtc acggcgtgtc actacgtggt
      181 cacattgatg tcacgacgga agatttagca agtgcgcttg atgtgacgga agacgtaatt
      241 gattcaattt tgcatgcaat gcaggggcgg gttctggatg gtgaccttat ttccggatgg
      301 gaaaaacgtc aggtgctgaa agaggacaat ggtaacgttt cgcaaacggc aaaatccccg
      361 gcagagcgca agagagcgca gcgggagcgg gaaaagctgc ggaaacataa tgctgattgt
      421 cacgatgagt cacgacgtgt cacgcatctg tcacgacaag tcacgacaga taaagataca
      481 gataaagata cagatacaga attaaacccc acacataacg cgcgcgagag tattccgacc
      541 agtgagtcga atggtgcgcc gttgcagaca gccgaacctg aatacctgga cggcctgagc
      601 gaaccgatcg ggaaattttc gatgactact gtctggcagc cgtcgccgga ttttcgacaa
      661 cgggcagcag tgtggggtat ggctctgcct gagccggaat ttacacctgc tgagcttgcc
      721 gcattccggg attactggat ggcggagggg aaggttttca cgcaggttca gtgggagcag
      781 aaatttgccc gccacgtgca gcacgtcagg gcacaggtaa aaccagtcag caagggggga
      841 agccatgcag catcaggtgg cacggcatca cgggcagttc aggaaatccg ggctgcacgc
      901 gaacagtggg aacgtgacaa cggatttatc agcaacggaa acggcctgga agctgtggga
      961 gctcatgggg gaggtgtatt cgaaccgctg gactcagaag aacggggccg caccttcgaa
     1021 gctctggatt gcccagattg gtgcgatgac tgaacagcaa atccgtctgg tgtgccgtca
     1081 gtgcatggac cgctgccggg cgggtgaaac gtggcccccg gacctggctg agtttgttgc
     1141 gctgatttcg gagagtgggg caaatccatt tggtcttacg gtggatgccg tgatggaaga
     1201 gtaccggcgc tggcgcaatg aatcctggcg atacgacggg agtgataaat acccgtggcc
     1261 acagcctgtg ctgtaccaca tctgcctcga aatgcgtacc agagggattg agcgccagat
     1321 gacgcaaggt gagttaaaac gacttgcgga acggcaactg acgaaatggg caaagcatgt
     1381 tggtaacggg atgagtgttc cgccagtgcg acgacaactg gaaggggcga aacacccgca
     1441 agggccaacg ccaattgaac ggctgaaaca ggaatacgaa cgccggaagg cagctggttt
     1501 tatttgaatc tgagaaacga ttttgtcgga ggaaatatta atggaaaccg tatttgacgc
     1561 actgaaagca ctgaaaaaag cctcttcaca ggtagtggca tcgcgccttg gaatcagccg
     1621 cgaagatgct gtcaacgaac tgtggaaact gaagcgccgt ggtgaagcgg ataacaaggg
     1681 gtcgatgtgg tggctgactc agactggtga aagtgaacca gtgtcaccgg taccgaaagt
     1741 gacagcgcaa atgctgactg aggcgattga acatcatggc ccacaaacgg cggatgagct
     1801 ggcactgatg ttcgggatta cctcccgccg ggcgaattca tcactggcca tggcaatcag
     1861 caaagggcgt ctgattcgcg tgaatcaggg cggtaaattt cggtactgca taccgggcgc
     1921 tgatttaccg gcagagccga aagccgcatc cataacggaa acggatggta aagcctttcc
     1981 tcagccagca ggtgttgcgt taccagtcgg ggaagcggaa acacaggaag aaataaaaac
     2041 ggaaagtgtg gcggtcacag tgcagtcaca gccgtcgttc accagaaagc atccggatgg
     2101 tctgatttta ccatcgctgc atgtggctaa ccgcgagctg cgccgggcaa aaggtcaggt
     2161 tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg aacaagtgcc gggatattct
     2221 ccgggatatt accgccacca gagaacagca gcggtgagtg ggtggaagac gtggtgccgg
     2281 gcggaaatca tgatactccg gcagtgtgcg ggaacgatga aggtaaaaag cgttggcgca
     2341 cttatcggac gaactgaagc ggcagtgaga acgaaggcac gggagctggg catcagcatg
     2401 atgttacgtg gtgattttca cccgtcggca aaatattctc agcgtgatat tgagctggcg
     2461 cggcaactgc atcagagagg catgcaaaga agggaaattg ccagaaaatt aggcatgccg
     2521 ctgcgcatag tgaataacta cgtttatttc gacaggaggg tgtctgcgtg aaaatcctgt
     2581 atcaggatta cggcccggtg gggcaggtgg ttatcagcag tactgtaatg gagtttcgga
     2641 agcataaccg tgtggtggat gctgtgctgt taacctgtcc ggggatatcg gcgagtcgtg
     2701 caggtgtgtt tattatgaag acgaaattat atggcagtaa ggcgtggata aagaaggcgt
     2761 atcgtgtagc gttgcaggag gttaacagtg agtgaaatta aagaaatgcc ggtagttcgt
     2821 gacggatatg gctactggac acatcctgaa tatgaaaaat tctgtgatgg tcgggaatat
     2881 atttcaacgg aagagtttaa cgcctggatg gaggaaaata atcttcaata cgtcctctgc
     2941 ttcagagatg aaggatgtgc tgaccttgat gcgtgtgatg ctgatatttc tgcatgggaa
     3001 ccggaacgac cagagggcga tggctggttt attggttcca ttcatgatac ggaagatggc
     3061 ccggtttgtg tctggttgcg aaataaggct gaagcataaa ggcgataaac caactaacaa
     3121 ctaaatactg aagatttaaa tcagaaacga tttttattaa atccttaacc ggagggattc
     3181 ctgcaccctc agaacatcag gag
//
LOCUS       DAZFDC010000260         3184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFDC010000260 DAZFDC010000000
VERSION     DAZFDC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.262"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            526..756
                     /locus_tag="ACHJ48_004855"
     CDS             526..756
                     /locus_tag="ACHJ48_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710213.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            808..2169
                     /locus_tag="ACHJ48_004856"
     CDS             808..2169
                     /locus_tag="ACHJ48_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO4710214.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERGRSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLNYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPLVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2216..2779
                     /locus_tag="ACHJ48_004857"
     CDS             2216..2779
                     /locus_tag="ACHJ48_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO4710215.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIINPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(2865..>3184)
                     /locus_tag="ACHJ48_004858"
     CDS             complement(2865..>3184)
                     /locus_tag="ACHJ48_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus protein"
                     /protein_id="HFO4710216.1"
                     /translation="ATGTVAASPGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHKQ
                     ENNKTDSNQSSRKKKNSSSSSSSSSSSSSSMNNNIISNRQMTGLKRQKPTAECPRNVR
                     GGA"
BASE COUNT          723 a          880 c          926 g          655 t
ORIGIN      
        1 cctgctcatt gcctgcaggg tgccacacac ccgtatgagt ccctgtcgcc gggagctgat
       61 acagccacaa cctgccgcac tgactgtcca gggtaaatga acagcccaca aaatttttta
      121 cccagaaccc ggtaaaaaat tttgcgttct gcccaggaca ggtgtgtcag gccgtggcag
      181 tgatgcccct tgcggcgaag gttctcaaac gggtgaagtt cataccacag ggaatacagc
      241 agcaggttcc cgcgaaacgt caaaaccgcg gctcccgaaa ctcaacgggt gatgttcttt
      301 aaaaatcagg atttatttca ggctccggca atacgcattt atgtgttctg tcagtcatcc
      361 ggctgacagg taatgagaac gcaggaacag aaggaggaaa acaccggaaa gcgtcagatg
      421 cagatatgaa aaaagcaggt cagggacctg cttaatccac gccgtaagaa ttgtgcgacc
      481 aattttgacg gcattgttaa ccaatttgtc ataaggaatc catttatgac tgtaagcatt
      541 gtttcacctt ccgcagcggc tgtaaagccc cgccgtcatc cccgtttcag acgcgaagac
      601 atccccgctc cggaaattga ccccgtgctg aaagccttcg ggcgacacat agcccggagt
      661 tttcaccggg ggcgtggtgt acacatcccc gccatgaaaa acacggcttt cgggcaggtg
      721 ctccgcacac tcgaactgaa acgcgctttt aactgatgag gtgagccgcc ccgcgagggg
      781 cggcaacaga cggaaggaga acattttatg acatcagcag caatcaccat gaccgccccg
      841 gaagccgcca gccctgtgca gatgtaccgc gcgacctact caccggatga caacaaactg
      901 cgcctgtatg ccgtgtcacg tcttgacccg gagacgtata aaaaagtgca tgatgccggt
      961 tttcgctggg caccaaaaca ggcgctgttt gtcgcgccgg cctggacacc gggccgggaa
     1021 gacgtgctcc tctcacttgc cggagagatt gaggatgaag acagcacgct cgctgaacgt
     1081 caggaagcac gggcggagcg gtttaccgga tacagcggaa agcgggccag tgaatccgca
     1141 caggcacttg atgaagtgga aagactggcc gcgatgatcc cgcccggtca gcccattctt
     1201 gtggggcatc acagcgaacg ccgcgcccgt cgtgatgcgc agcgtattga aaacggcatg
     1261 aaacgtgccg tgatgctctt tgaacgtgcg gaatactggg aagaacgggg gcggtcagca
     1321 ctgcttcacg cgaagtataa agaacgtccg gacgttcgct ggcgtcgtat caaaaaaatc
     1381 gaagctgatt tgcgcaaggc tgaaaagacc atcgcgcagt cgcagaaata tctgacgatg
     1441 tggcgggctg aatcgctgga tctgaatatg gcaaaactca tcagcagtca tgaccatatc
     1501 agcgcctgtt tcccgctgga tacgtatccg cgcccggcag aaaaaagcca gtatgaaggg
     1561 agccggtcgt tatggtcggc cctggatgat gacatcatca ccacggagca ggcccgcgaa
     1621 attgcgatcc gctgtcatga acggcagatt cagcatcagc aacgctgggt taaccactat
     1681 cagaaccgcc tgaactatga gcgtgccatg ctggacgaaa gcggcggcgt ggttacccgg
     1741 acacaggatt ttgagccggg cggacaggtt ttcagccggg gcgagtggct gaccatcatc
     1801 cgcgtgaaca aaagcaacgg ggcggtgagt tcagtcacaa cgccgaatta cagttttctc
     1861 gggtacagcg gcacgatgaa agtgacgccc gatcgcatca cggactacaa agcaccatcg
     1921 gcagaagagg ctgccgtcgc cagccaggcc gcgaagcgtc cgctggtagt caactatccg
     1981 ggggaaggtt tccgggaaat gacaaaggca cagtgggccg ccctgccccg ggactgtaag
     2041 gccgtgcgca gtgtggcaga agcagaagac cacggggcat accgctaccg ccgcacaatg
     2101 gacaataatt tccgtctggt gaatgtgtat atcaccgaca tgaaaattac ggaaatccca
     2161 cagaaataag gtatatcccc ccgggaaatc cccgggggat gacggagata atgacatgca
     2221 cagtcagtta aaagaacgta tccggctgat gcgcgcaagg ctggataacg ccgcgccggt
     2281 tgctgaaatc cgggctgaat ctcagctttt tgtgactcct gccccggtct gtgatcgcct
     2341 ggtgacgctg gcggagatca gcaaccgtga tcacattctg gaaccctctg ccggcaccgg
     2401 ggccatactg cgggcgattc gggataccgc accggaggcc atgtgtgatg cggtggaaat
     2461 caacagcggg ctggtccggt atctgcggga aaattttaac ggtgtcaggg ttcagtgtgg
     2521 cgacttcatg gaatggcagc cggtgcagta ttacagccgg gttatcatta atccgccgtt
     2581 cagccacggg caggatatcc ggcatatcct gcgggccttt tccctgttgc gtccgggtgg
     2641 cgtgctggtc gccgtctgtc tgaacgggcc gcgccagcag gagaagctgt taccgttttc
     2701 tgacgtccgc gaggagctgc cacgcggcac gtttgcttat accgatgttc cgacgatgat
     2761 tattcgtctg cgtgcctgat gactccgggc aggcgggagg ataaatgccc gtctgcccgc
     2821 gccgggaaaa gaccgcgcgc cggtggcgct gaaaggggcc gcgctcacgc gccgcccctt
     2881 acattacgcg gacattctgc tgtgggcttc tgtcttttca gacctgtcat ttgtctgtta
     2941 ctgatgatgt tgttgttcat gctactgcta ctgctactgc tactgctact gctactgcta
     3001 ctgttcttct tttttctgct gctctgatta ctgtctgttt tgttgttctc ctgcttatgc
     3061 cttttccggg cgtccccctg gcggggcggg ctgtcgtgaa cggagccagc ggagctgtct
     3121 ccgccctggc gggcttccat ccctgacgcg aaacccgggc ttgcagcgac cgttccggtc
     3181 gcgc
//
LOCUS       DAZFDC010000261         3165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFDC010000261 DAZFDC010000000
VERSION     DAZFDC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.263"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(8..634)
                     /gene="dsbA"
                     /locus_tag="ACHJ48_004859"
     CDS             complement(8..634)
                     /gene="dsbA"
                     /locus_tag="ACHJ48_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO4710217.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(651..1637)
                     /gene="srkA"
                     /locus_tag="ACHJ48_004860"
     CDS             complement(651..1637)
                     /gene="srkA"
                     /locus_tag="ACHJ48_004860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO4710218.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1714..1983)
                     /locus_tag="ACHJ48_004861"
     CDS             complement(1714..1983)
                     /locus_tag="ACHJ48_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO4710219.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2053..2637
                     /gene="mobA"
                     /locus_tag="ACHJ48_004862"
     CDS             2053..2637
                     /gene="mobA"
                     /locus_tag="ACHJ48_004862"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO4710220.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIAMVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2619..3146
                     /gene="mobB"
                     /locus_tag="ACHJ48_004863"
     CDS             2619..3146
                     /gene="mobB"
                     /locus_tag="ACHJ48_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO4710221.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
BASE COUNT          828 a          769 c          763 g          805 t
ORIGIN      
        1 cattgaatta ttttttctcg gacagatatt tcactgtatc agcatactgc tgaacaaaaa
       61 catccatatt gctggtatcc ataccctgcg gattcagctg atatttaccg ttaacaaaca
      121 tcgccggaac accacgcaat tgcacgtcag ctgcagcttt ttcctgctga gcgaccagag
      181 atttcaccac gaagctgttc cacgccgcgt cgtactcttc acctttaata cctgcgttga
      241 taaatacatc gcggatatca gatgctgaac gaatggtctg ggttttctgt acgccttcaa
      301 acagcggaac agtcactttg tcttccacgc ccagcgccat cgccacagcc catgcctgag
      361 tcagatcttt gcccaggtca ccacccatga agttgacgtg gtatttagtc atcttcacgc
      421 cttccggcag ttttttcttc acattatcag aaatatgcag aacttcttca aactgatagc
      481 agtgcgggca gaagaaagag aaaaactcca gcacttgcgg cgcgccagct accggttttt
      541 ccagggtagt gtactgttta ccatcttcat actgcgccgc cgatgcgcta aacgctaaaa
      601 ctaaaccagc cagcgccagc caaatctttt tcatgatcta ctctctccga ttaatacata
      661 ggtgttaatt gcaaaggggg ttcttgtaga acttttgcct gttctataaa agtcgccgtc
      721 tgtcgtagcc agtaatcttc cccggttaac cacgggaaat ttttcgggaa cgcgggatca
      781 gcccaacgcc gcattagcca ggcaagataa taaaccaaac gcatggcgcg taaaggttca
      841 atcagtccga tttcagcggt gtcgaactcg ctaaattctt cataagcttc aataatagtt
      901 tccagttgca tccgctgctc ggctttatcg ccattgagca acatccacaa atcctgaata
      961 gctggaccat tacgtgcatc atccagatca acaaacattg gaccatcgcg ccagagaata
     1021 ttcccggcgt ggcagtctcc atgtagccgc agaacggtga aatcttcccg ccagtgtgct
     1081 gtaacggcgg cgatcagctc atctgtcgct ttcaggaatg ccgctttcaa cccggaaggt
     1141 atcagtgtag cgtcctcaaa cagcttgcgt ggctcaatga gatattcatt cagaccgatg
     1201 gtcgggcgat ggataaaaag ctgtttgcgc cccgtctggt gcatacgccc taaataacgc
     1261 ccaaccgctt ccatctgatc gatattatca gcttcgaact ggcgaccacc gacgcttgga
     1321 aaaacagcga aataaaatcc ctgatgattc aataaagtct gaccgttaaa agccacaggt
     1381 gctgcaaccg gaacttcatc atttaccagc tgcaacgcaa attgatgttc ttcgaggatt
     1441 tgatcggctg tccaacgttc aggacgataa aatttgacga caaaacgtcg acgatcttcg
     1501 tcctgaaatt gatagacacg gttttcatag ctgttaagcg gggtaagacc ggaatccacc
     1561 cggatccctt gctcaaacag agcgtccatg atggtatccg ggtgtagtgt ctggaaagta
     1621 aaagcgctgt tgttcatccc atcatccgga aaacattacg aatgatgaag gatatcattt
     1681 cacgatgatt ttggtggcgc cgcttacaag cttttactct ttaattacgc cgcgagcgcg
     1741 taacagcgcc gttttgaagt cttcttcata atctttctgc aaacccggga tcaccgcatc
     1801 tttcgcggaa tcacgcattt tcaggtgata gatcagaatg tcatccgtca aatccgccag
     1861 ttcgccgtca aaacctgact ctttcgccag tttctgcaaa aattgcaaca ggttaaggtc
     1921 tggctctttt tgccaggctg gctggaggag ttcaataact tcattcagac gtttacattt
     1981 catagtaatg ctccttactc ttgagacaga cacgttagca gggtcaatcc cacaataaaa
     2041 gaggcgatat cggtgaatct gatgacgacg ataacaggcg ttgtgctggc aggcggtaaa
     2101 gccagacgaa tgggcggcgt agataaagga ttgctggact taaatggcaa accgctgtgg
     2161 caacatgtcg ctgacgcgct tatgacgcag ctctctcacg tcgtggttaa tgctaatcgt
     2221 catcaggaaa tctatcaggc aggcggtctg aaagtgattg aagattcact ggcggattac
     2281 ccaggccctc tggcagggat gctttcagta atgcagcagg aagctggtga gtggtttttg
     2341 ttttgtccgt gcgatacgcc ttacattccc catgatttag ccgcccggct taatcatcag
     2401 cgcaaagatg cgcctgtcgt gtgggtccat gacggtgaac gcgatcaccc gactattgct
     2461 atggtaaacc gcgctattga gcctttatta ctagaatatc tgcaagcagg agaacgccgg
     2521 gtaatggcat ttatgcgtct ggctggcggt catgcggttg atttcagcga tcataaagat
     2581 gcatttgtta acgtgaatac gccagaggag cttgcccgat ggcaggaaaa acgatgatac
     2641 cgttactcgc ctttgccgcg tggagtggta ccggaaaaac tacgcttttg aaaaaactga
     2701 tcccggcatt atgcgccaga gggatccgtc cagggctgat taagcatacg caccatgata
     2761 tggatgttga taagccaggc aaagatagct atgagctgcg caaggctggc gcggcacaaa
     2821 ccatcgttgc cagccagcaa cgatgggcct tgatgacgga aacaccagac gaagaagagc
     2881 tggatctaca gtttctcgca agtcgaatgg atacctcaaa gctggatttg attctggtcg
     2941 aagggtttaa gcatgaagag atcgcaaaga ttgtgctgtt tcgcgatgga gccggacatc
     3001 gacctgaaga attagtgata gacaggcatg ttattgctgt agccagtgat gtgccgctta
     3061 atcttgatgt cgcattactg gatattaatg atgttgaggg gctggctgat tttgtggtgg
     3121 agtggatgca aaatcaggac ggataagcaa gagtaggctg gagca
//
LOCUS       DAZFDC010000262         3162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFDC010000262 DAZFDC010000000
VERSION     DAZFDC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.264"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(274..1752)
                     /locus_tag="ACHJ48_004864"
     CDS             complement(274..1752)
                     /locus_tag="ACHJ48_004864"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO4710222.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(1779..3056)
                     /locus_tag="ACHJ48_004865"
     CDS             complement(1779..3056)
                     /locus_tag="ACHJ48_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO4710223.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTQPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
BASE COUNT          847 a          808 c          713 g          794 t
ORIGIN      
        1 gaataaagat atccagttca tactttaagt gaaaattgat aaagtgcgat tcgtattgtc
       61 ttttattcta aagatatcga gtgtagttaa tattccttgt aaaaacaggg ataaaccgaa
      121 ctagttaaag tttttattat gttccctgca tcagcgggga taaaccggac ttgttatcat
      181 tttttaagtt gtcgaactta tgttccccgc gccagcgggg ataaccattc aggtatgttc
      241 aggccgggca gtttcccgcc cggtcttcct cacttacgca ttcgaccagc tctggatcga
      301 atccgcattc cccaattccc catgcatcac ttcatacgat tttttcagga tcacatcagt
      361 atgtttggtt agggctttga aaataccttt attcatcgcg tcgtagcgtt tggcattgct
      421 ctcaatcggt ataaagatat ctttcacgcg aaccattttg tcgacagccg ttgcgtaatc
      481 cgggtatagc cccagaccta ccgccgtatt gatcgctgcc cccagacttg cacaaccgtt
      541 aatggcgtta cgtcgtgccg gaaggttgaa cacgtcggca aaaatctgca taaacagatc
      601 gctgttcgaa ccgccaccag taatgatcac atgcttcgca aagtgattca tttcattaca
      661 catattgtcg taattgttct tcagcgtcag cgccacgctt tccagtatcg aacgatatat
      721 ccatgcgtaa tccatgctgg aatcaaagcc gatcataatc ccgcgtttgt acggttccca
      781 cggattggtc agccagtcca gcaccgtcat cagcccatta cagccaggtg gcacacaaga
      841 agcttttttg ttgagtaaat cttccggtga aagatcctgc gccctggcat cctgaattaa
      901 cgactcgcct aacatgtcgc gcagccagct caccgtccac atgcctttgc gaataccgta
      961 accttcatac agcaatgttt gcggaataga agacataatc ggccagtacg ccaccggatc
     1021 tttcggcaag gctttgccgt tcatcatcag cgcgatataa gtaccgagtg aaatcaccgc
     1081 cgtttcatca tccagtaatc cggctcccag agcttctacc ggtttatcac tggtggtaca
     1141 aacaaccggc agccctgccg ggaaatgtgt cgccagcgcg gcttgtggtg tgatctgtcc
     1201 aagaacagtg cctggcattt gcacatcaaa cagcatatga cgggggatat taaacttctc
     1261 cattaccgca gcatcttcgc tccatgccca gctcttataa tccaccggcc actgaccaaa
     1321 atagttggcg atattgtctt taaactcgcc ggttaagcga tgcgtcagat aacccgaaaa
     1381 agaggtgaca taggccacgt caggattagt gtgttcgtag ggacgcgtta cgcgtgcatc
     1441 ctgccagcta atcaacggcg cagcaggcgt gccatcggct ttcaataacg cacggcagca
     1501 acggatggat cccagaccaa taccgacaat atcttcttta ttcccggcaa actgactcat
     1561 caaatcgtga ccggcaaaac ataatgatgc ccataaatcg tcgtcaggat gttctgcggt
     1621 atcggcatct ggcgtgtgca tcggctgcaa taaacctttg ccttcgcaaa ccacgttacc
     1681 ttccagatcg tacatcacca cttttgtgct ctgacttccg ccatcaatcc ctatgatgta
     1741 tttcttcgac atgatttttc tcctgtaatt ttcctggctt acttgctgct ggccattgcg
     1801 ggggcgctat cagcagtacg aattttttgg aacagcaaaa aggtaaagac aatcaccatg
     1861 gcaagcgccg ccatgcccat cagccacata ttgcggtagg cttctgctgc gggctgggtg
     1921 tcttgccagt ggccgataat tgggtaaacg aagacatccg gcaggaatcc aatcaccgag
     1981 caaataccta ccgtggtgcc cataatgtaa gacggtgttc tcgcttcgcc agggcaggcc
     2041 cagtacagcc cgcgtgaggc gtaacaggtg aatcccagca gtaaaatcag gccaatcccc
     2101 atcgcgaccg attgcgggtt agagttcgtg acgagcaggg cagttaacgc cagcaggccg
     2161 agtacggaaa ggatttggat cacgcgggta ggggatttca ctttactgta ggtggtgata
     2221 atgccgccaa gtgggccgca cagcgcgcgg aagattttgt tgatcacaat ccccatgtag
     2281 ctcgccgcca ccagcgacat gccatacatt tcggtcagat agttggtgga gtaactcaga
     2341 atggcgtaga tggtgaagac gccaaaaatc accatgctgc aataccaggt ggtgctgata
     2401 cgcaaaacgg caaggatgtc gctaagctgg aatgactgtt tttcttcgtt attggcactg
     2461 cgcaggttgt tgttatcgct aacaaaaaac cagcacagaa tccccaacaa gatgtaaacc
     2521 acactataga tgataatgac cgttttcagg ctggtgctgt catccggtgc aaagcgagaa
     2581 aaaacccaca tggtaaacac tgccagcgac attacaccga cgccgcgcag cccttccatc
     2641 cagcccataa ttttcccttg ctcgctatga tcgccaagca acgatgcggc tttaatcgac
     2701 accgaccaca gcattaaaat cgttgttatc gcaaaggcga cctgaataca gagcattacc
     2761 cacagcggtg gatacgttgc cattaacaga cccagtaatc cggtaatgat catcgcggaa
     2821 gtaatcattt tgcggtgtga aaatttatcg gcaataacgc cgctgggggc ataaagaata
     2881 atagccgcaa taccaaaggt actcattatt aaacctatct cggtattgct gaatcccata
     2941 aatttcgcca tgggaatttg ataaatataa cgtaaataag ccaggtcgaa actaacgccg
     3001 ccgctaaaac taattatcgc gagggttatc caacggcgat atgagttgtg ttgcatatcg
     3061 tattcccatt tgtaatagac aaaaaaaaga gaaaagtaag ccgccctttc aggtggatta
     3121 cttttctcag gtctctagta attgaaataa taaataacat gg
//
LOCUS       DAZFDC010000263         3161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFDC010000263 DAZFDC010000000
VERSION     DAZFDC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.265"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..315
                     /locus_tag="ACHJ48_004866"
     CDS             <1..315
                     /locus_tag="ACHJ48_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4710224.1"
                     /translation="EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTN
                     TELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSL
                     AG"
     gene            complement(358..1509)
                     /locus_tag="ACHJ48_004867"
     CDS             complement(358..1509)
                     /locus_tag="ACHJ48_004867"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO4710225.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1523..3118)
                     /locus_tag="ACHJ48_004868"
     CDS             complement(1523..3118)
                     /locus_tag="ACHJ48_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO4710226.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT          784 a          808 c          721 g          848 t
ORIGIN      
        1 gaacagttat cgaaaaaatt tcgtgccagc gtcagttata tttgccatga atttaccaaa
       61 gagtatcgta tttcccctat taactatgtt attcaacggc gtatgacgga agcaaaatgg
      121 tcactcacta atactgaatt atcacaggca gagatctcct ggcgtgtggg ttatgaaaat
      181 gtcgatcact ttgccaaact gtttttgcgc catgtcggct gttcgcccag cgattaccgc
      241 aggcaattta aaaactgttt tgcggaacaa gaaatcctat ctgaatttcc tcaaccggta
      301 agtcttgccg gataaatctg gcggcgcagt ttgttaagaa gctgcgccgc ctgcagatta
      361 tttgttctga tagtctttca ggatctgccg acctgctacg taaatcataa tttcgtctgt
      421 accgccgcca atacgttcac aacggacatc acgccagaag cgggagacgc gcgcctcatc
      481 ggtatagccc agaccgccca tgatttgaat cgcatcatca atgacttcca ttgcggtacg
      541 tgcacagtac agttttgcca gcgccgcgct ggtgcgcagt gattgatgct gatcggcttg
      601 ccatgccact ttcagcacca tgttgcgcat gttgtcaatc ttaatcgcca tcagcgccag
      661 tttttcctgg atcatctggt tatgaccaat gggtttacca aaagcgatac gctggttggc
      721 atagcgagcg gcatcttcaa aggcacactc ggcaaagccg gtgctgcgcg cggcgttgat
      781 caggcgctcc atctcaaagt tgtacatcac attgaggaaa cccattcctt cttcgcccac
      841 catgtcgctc tcttcaactt ccacgttgtc gagatagact tcgcaggtgc tgagcatatg
      901 ccagccgatt ttatgcagcg ggttaatctt aatgccgggc ttactggagt cgacccacca
      961 cagggtgaag gcttttttgg gatctttcgg ttgcggatcg cgcgccagca ccagcatata
     1021 cgggtactct ttcgcaccgg taataaaggt tttctgtccg ttgatgtaaa ccttgccgtt
     1081 tttacgcgtg taagtggtag tggcactgtt gttatctgag ccagcacctg gttccgtcaa
     1141 cgccagggca taggcgggat cacccgtttc cagggtgctt tctgccgttt tacgtagctg
     1201 ctctgcagaa ccgaaacggc gcatactgtg aatacattga ccgttggtaa ttaaaaatgc
     1261 cggagcaccg cattttgaca cttccatcag cgccagcatt tgggtgacgt aatcagcagg
     1321 gataccacca aattcttccg gcacgccaag catggaaata ccgttatccg ccagtgcccg
     1381 cataaactca cgcggatatg tcccgttttg atcgcaggtg cggaaatact cttccggaaa
     1441 gttagtcgta atcagttcgc gaatactggc cagcagcagt tcttgttctt cagttaaaga
     1501 aaaatccatc gtgatactcc tgttaatgag ccgcttcagg caggacaaaa cccatcgccg
     1561 catcgataaa taatctttcg tccatcagtt tgagttctgg cgaaatcact ggggtgaaat
     1621 ccattttgtc gagaatatct ttttgtaaat cgacgccagg ggcgatttcg attaaatgca
     1681 gtccgtcttc tttcagcgtg aatacggcgc gctcagtgat ataacgaaca tccagccctc
     1741 gctcgagagc gatttttccg ctaaaagtaa tttccggtag ttcccgaata aatttcttca
     1801 cccgtccttc ctggacgata ttcaatttgc catcggcaat ttctgttttt aaactgcccg
     1861 cagttaacgt gccgcagaaa ataattttct tcgaggtggc actgatatca ataaatccac
     1921 cggtacccat gattttgcca ttgaatttat gcacgccgac gttaccgtgc tggtcgactt
     1981 cagcaaaact caaataacaa acatccagac caccaccgtg ataaaaatca aactgggacg
     2041 tcatatccag aatggcacgg gtattcacgt tcgcgccaaa ggcgataccc tgtgaagtaa
     2101 ttccaccaat cggacctgtt tctaccgtca gaataaagtc atcagcacaa ccttcttccc
     2161 gggcgaccag gccaatgccg tcagcaatac cgacgccgac attccccacc gcgcctttac
     2221 gcatttcgaa taatgcacgc cgtgcaacta atttacgttg gtttaggggc aggctaagtt
     2281 tggtgctgtc atcaagggtg aagtcaccag aaataaagcg gttaaccggc gcaccgccat
     2341 atagttggct ttgatccgga tcgaccacca caatatccac cagataaccc ggaatacgga
     2401 cagatttagg atgcagtgtg gctttcttaa ccattttctg cacctgcatc atcacaatac
     2461 cgccgttatt gtgcaccgcc tgggcaataa ccaatgcgtc gagatacatc acctcatctt
     2521 caaaagtggc gtagccttca ctgtcgcagg tggtagcgcg aatgaaagca atatctggcg
     2581 caattgcttt gtaatagaga tattctttgt tatcaaactc gaccagttta atcaggtctt
     2641 ctttagtgac ttcattcagt ttgccgcctt gctggcgtgg atcgacaaat gtcccgatgc
     2701 caatatcact aataatacca ggctggtggg ctgcggcggc gcgtaaggtt tgtgtaagta
     2761 caccttgtgg atagttataa gcaataattt tattttgttc tgcgagatca gaaatacgcg
     2821 gcgattgtcc ccagtgaccg cataatgccc atttcaccag accttcttgc gccagcggac
     2881 taataccgcg gtcggcgcga tcgccaagcc ctgttggact aataatcgat aaattacgtg
     2941 gcgtttgagt ctgtttatat ttatcagcaa gagcagtaat taacgtggtg gcttccagaa
     3001 taccgccgcc agcgcctaac acacaaagtg ttgcttcgtc gggaatataa ttcaccgctt
     3061 cctgtgccga caggaccggc actcgtccat taatacgagg tggttttaca ggtttcattg
     3121 ttaattcctg attcaggtgt gattttccct acgccagatg g
//
LOCUS       DAZFDC010000264         3147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFDC010000264 DAZFDC010000000
VERSION     DAZFDC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.266"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            404..592
                     /gene="dicB"
                     /locus_tag="ACHJ48_004869"
     CDS             404..592
                     /gene="dicB"
                     /locus_tag="ACHJ48_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO4710227.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            589..780
                     /locus_tag="ACHJ48_004870"
     CDS             589..780
                     /locus_tag="ACHJ48_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO4710228.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            873..>3147
                     /locus_tag="ACHJ48_004871"
     CDS             873..>3147
                     /locus_tag="ACHJ48_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO4710229.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETESDPM
                     NVTFDNLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKEAGKYTSVVDLARA
                     RANQQHTENSTGKINPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNGREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNSEQ
                     PEPVKVTADEVKKIMQAANISQPDAEELLAVSRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCG
                     AVMGDATYQEIFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPL
                     IKANGHHNLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LDTAGGVIDRDTMKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWG
                     NGANFDNTILRRSYERQG"
BASE COUNT          935 a          727 c          781 g          704 t
ORIGIN      
        1 catagatagc ctgtcgttaa attttcgtcg accgtgcgct tccggttgtg gcaatccgcg
       61 aaatggcgcg gcggtaagta tggaggggtt attccttccc ccgttgagga caccgggttg
      121 tcaggttgac catacgctta agtgacaacc ccgctgcaac gccctctgtt atcaattttc
      181 tggtgacgtt tggcggtatc agttttactc cgtgactgct ctgccgccct ttttaaagtg
      241 aattttgtga tgcggtgaat gcggctgagc gcacgcggaa cagttaaaac caaaaacagt
      301 gttatggggg gattctctgt atccggcgtt aattgttaac tggttaacgt cacctggagg
      361 caccaggcac cgcatcacaa aattcattgt tgaggacgcg ataatggaaa cgttattacc
      421 aaacgttaat acgtctgaag gttgttttga aattggtgtc actatcagta accctgtatt
      481 tactgaagat gccattaaca agagaaaaca cgaacgggag ctattaaata aaatatgcat
      541 tctttcaatg ctggcccgtt tacgtccgat acaaaaagga tgctggcaat gaatacagca
      601 tttgcacttg ttctgacagt ttttcttgtt tccggagagc cagttgatat tgcagtcagt
      661 gttcacagga caatgcagga gtgtgtgact gcagcaaccg aacagaaaat tcccggtaac
      721 tgttacccgg tcgataaagt tattcaccag gataataacg aaatcccggc aggtctttaa
      781 aacagttccg taataaacat ccgatttcat tcttatatgc cagcaatggc agggatttgt
      841 tcacccttaa atctgtaatg aggtaaaaca aaatgagtaa agtctttatt tgcgccgcca
      901 ttccggacga acaggcaata aaggaagaag gtgccgtcgc tgtagccact gccattgaag
      961 ccggtgatga acgtcgcgcc cgcgcaaaat ttcactggca attcctggaa cattatccgg
     1021 ctgctcagga ctgcgcttat aaatttcttg tttgcgagga taaacccggt ataccccgcc
     1081 ctgccctcga ttcctgggat gctgaatata tgcaggaaaa ccgctgggat gaggcgtctg
     1141 cttcctttgt cccggtcgag actgaatccg atccgatgaa cgtcactttt gacaacctgg
     1201 cccctgaagt acagaacgct gtcatggtta agttcgacac atgtgaaaac atcactgttg
     1261 atatggtgat tagcgcacag gaattgttgc aggaagacat ggcaacattc gacggccata
     1321 tcgttgaagc gttgatgaaa atgccagaag ttaacgctat gtatccggag cttaagctgc
     1381 atgccatcgg gtgggttaag cataaatgta agcctggtgc caaatggccc gaaattcagg
     1441 cagagatgcg catctggaaa aaacgtcgcg aaggtgaacg caaggaagcc ggaaaataca
     1501 cgtctgttgt tgatctcgcc cgcgccagag ccaatcaaca gcacactgaa aattcaacag
     1561 gaaaaatcaa cccggtcatt gctgccattc atcgcgaata caagcagaca tggaaaacac
     1621 tggatgacga actggcctac gctctctggc ctggtgatgt ggatgccgga aacattgacg
     1681 gcagcatcca tcgctgggca aaaaatgaag ttatcgacaa cggccgcgaa gactggaagc
     1741 gtatctcggc atcaatgcgc aaacagcctg atgcccttcg ctacgaccgc cagactattt
     1801 ttggccttgt ccgtgaacgt ccgatcgaca ttcacaaaga ccctgtggca ctgaacaaat
     1861 acattactga atacctgact acaaagggcg tgtttgaaga tgaaggaaca aatcagagcg
     1921 caactgatac tctctcgtcg ccagtaccag aaactgatgc agtggaaacg gcaattccag
     1981 acaacgaaaa aaccgaatgc aaagtggaag tcgaaccatc tgtagaacgt gaggggccgt
     2041 tctacttcct cttcaccgac aaggatggcg aaaaatacgg tcgcgcaaac aaactttctg
     2101 gtctggataa ggcgctggct gccggggcta ctgaaatcac aaaagaagaa tattttgccc
     2161 gaaaaaatgg cacatacaca ggcttaccgc aaaatgcaaa taccgcacaa aattctgaac
     2221 aaccagaacc ggtaaaagtt accgctgacg aagtaaagaa aattatgcag gcagccaata
     2281 tcagccagcc tgacgccgag gaactgcttg cagtatcacg tggtgaattt gttgaaggga
     2341 ttagcgaccc gaatgatccg aaatgggtta aggggatcca gacccgcgat tctgtgaacc
     2401 agaaccagca agaaacggaa cagaacgacc agaaagcgga acaaaacagc ccaaatacgc
     2461 aacaaaacga gccagaaacg aaacaacctg aaccagtagt gcaacaggaa ccggaaaaga
     2521 tctgcaccgc ctgcggtcag agcggtggcg gcaactgccc tgattgtggt gcggtgatgg
     2581 gtgacgcaac ataccaggaa atattcgatg gagagaatca gcctgaagtt caggaaaatg
     2641 atccggagga aatggaaggt actgcacatc agcacaagga gaacactggc ggcaatcagc
     2701 atcatgccag cgatagtgaa actggcgagg cgtcagatcc cttaattaag gcgaacggtc
     2761 atcataatct cacatccacc agcagagcgg ggattcatct gatgatcgat cttgaaacca
     2821 tgggaaaaaa tcccgatgcc ccgattatct caataggtgc aatatttttc gatccgcaaa
     2881 ccggagatat gggaccggaa tttagtaaga ctatcgatct ggatactgct ggcggagtca
     2941 ttgatcggga caccatgaaa tggtggctta aacaatcacg cgaagcgcaa tctgccatta
     3001 tgaccgatga aatcccgtta gatgatgcac tgttacaatt gcgggaattt atcgacgaaa
     3061 actccggtga attttttgtt catgtctggg gaaatggagc caacttcgac aacacgattt
     3121 tgcgccgttc atacgaacgg cagggga
//
LOCUS       DAZFDC010000265         3100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFDC010000265 DAZFDC010000000
VERSION     DAZFDC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.267"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..112)
                     /locus_tag="ACHJ48_004872"
     CDS             complement(<1..112)
                     /locus_tag="ACHJ48_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4710230.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGERSGA"
     gene            complement(124..522)
                     /locus_tag="ACHJ48_004873"
     CDS             complement(124..522)
                     /locus_tag="ACHJ48_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO4710231.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHVTRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(564..1589)
                     /locus_tag="ACHJ48_004874"
     CDS             complement(564..1589)
                     /locus_tag="ACHJ48_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO4710232.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(1645..1977)
                     /locus_tag="ACHJ48_004875"
     CDS             complement(1645..1977)
                     /locus_tag="ACHJ48_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO4710233.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(1987..>3100)
                     /locus_tag="ACHJ48_004876"
     CDS             complement(1987..>3100)
                     /locus_tag="ACHJ48_004876"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4710234.1"
                     /translation="PRQARSYQVMNGIAVLPVSGTLVSRTRALQPYSGMTGYNGIIAR
                     LQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALANDMNCSAGQLL
                     ASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHKVDGNPYSHLPG
                     DVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAIDAGLADELVNST
                     DAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPATEGENASAAQPD
                     VNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETARRILAAAPQSAQ
                     ARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
BASE COUNT          607 a          950 c          862 g          681 t
ORIGIN      
        1 ctgcgccgga ccgctcaccg gatgtcatgg tggctgacgt tcccatgtac ccgcgtatcg
       61 tttcatcggc gcgggcaatg gcagcatcga acaggttatc gaaatcagcc acagcgcctc
      121 ccgttattgc attctggcca ggccgcgctc tgtcatttcg gctgccacac cggcagagac
      181 acgaaacgcc gttcccggca gcacaaatgc cacaggttca tcccgcgtga cgtgaagtgc
      241 atcagtatgc agcttcacca gtgccacgac cgtgaccagt tcagacgtat ccagaatcac
      301 ggtatccggc tgcgctgatc ccacctcatt ttcatgtccg gtcagcacat tttcccggct
      361 gagaggggtg tcctgaccgg cagtttcatc cgtgtcatca agctcctctt ccagctctgc
      421 cacacggagc gccagttctt ctttcgtccc cgtcaggctg acatcacggt tcagttgttc
      481 acccagcgag cggagacggg caatcagttc atctttcgtc atggactcct ccacagaaaa
      541 acaatggccc cgaagggcca tgattacgcc agttgtacgg acacgaactc atcagggtca
      601 gccagcagca tcagcggtgc tgactgaatc atggtgaact cacgcgccgg atcgccggtg
      661 gtcacccagt ttttcgggta acgggcagag gcgttaatgc cttcgcgctg tgcgtccgca
      721 tcctgaatgc agccataggt gcgcagaccg cgtgcctgag tgttccccag caccatcgtg
      781 ttgtccggca ggaagttctt tttgacgccg ttttccacgt actgtccgga atacacgacg
      841 atggccacat cgccatacat tcccttataa gacaccgctt tgcccaggtc ttttaccgct
      901 gtctccagtt cggaatgaga gccgcgacgg gtatccagct tctccctgac ggctttgaag
      961 gaacggaaca gcgcccagcc tttcgggtca aacacgatga tattcaccac gccgctggcg
     1021 ttcagcgcgt aggcttcgat atcgtcggtc gggtcatacg tggacttgtc acgcttgctc
     1081 cactccgtgc cgccggactg cgtgatgttg ttcgccgcac tgcggcccat atccacctca
     1141 accggatcga aggcttcacc ggtcatggtg tatttgccct tgagcacggc agaaactgcc
     1201 tgcatctctt cgacctgagc aatggccagc tcttcgtcac gcatgttctg catgatgatg
     1261 cgacggcggc ggtaagccgg gtccgccaga ttctgcggat cttcatccgg caggcgacgc
     1321 agggtcatct gcggattcac ttcatgcttc ggcttgacat atcccggcgt aaattcagag
     1381 gtggagccgc cacgggaacg gataacctca ccggaaacaa tcggcgaaac gtacagcgcc
     1441 atgtttacca gtcccggaat ttgtgagaga tagactttct ccgtggtgaa gggatagctc
     1501 tcacggaaaa agagacgcag aaacagcgga tcaaacttaa atttctgctc atttgccgcc
     1561 agcagttggg cggttgtgta catcgacata aaaaaatccc gtaaaaaaag ccgcacaggc
     1621 ggcctttagt gatgaagggt caggttaaac gatgctgatt gccgttccgg caaacgcggt
     1681 ccgttttttc gtctcgtcgc tggcagcctc cggccagagc acatcctcat aacggaacgt
     1741 gccggacttg tagaacgtca gcgtggtgct ggtctggtca gcagcaaccg caagaatgcc
     1801 aacggcagca ccgtcggtgg tgccatccca cgcaaccagc ttacggctgg aggtgtccag
     1861 catcagcggg gtcattgcag gcgctttcgc actcaatccg ccgggcgcgg ttgcggtatg
     1921 agccgggtca ctgttgccca gcggctggta atgggtaaag gtttctttgc tcgtcataaa
     1981 catcccttac actggtgtgt tcagcaaatc gttaacggca tcagatgccg ggttacctgc
     2041 agccagcggt gccggtgccc cctgcatcag acgatccagc gcagtatcac tgcgcgcctg
     2101 tgcactctgt ggtgctgcgg ccagaatgcg gcgggccgtt tccacggtca taccgggggt
     2161 ttctgccagc acgcgggcct gttcttcgcg tccgtgagcc tcctcacagt tgaggatccc
     2221 cataatgcgg ctgttttctg ccgcaaccgc agcggtgatc tgcgcgttca cgtccggctg
     2281 cgccgcgctg gcgttttcgc cctccgtcgc tggcaccacg ccagtaacgt cagcctgcga
     2341 agcagtggct gaaacagttg ttgattgagt ctctttggtc attcgccctc ctgagagacg
     2401 ggatttacgt gcatccagtg catcacgcat aacggtgatc gcatcggtgc tgttgacaag
     2461 ttcatcagcc agtccggcat caatggcctc ctgaccgctg tacactgcag cctcggtatc
     2521 cagcacagcc tgcacggaca ggccggtata tgccgacacc ttctgcgcaa acatccggcg
     2581 ggttgcatcc atccgggact gcagtgtttc ccggacatca cccggtagat ggctgtaggg
     2641 gttgccatcc accttatggc tgccgctgta aatcagcgtg atttccacgc cctgtttctc
     2701 cagggcagca ccgtaattac tgtgagccat catgacgccg atggagcctg tccgggcggt
     2761 ctgcgtgacc agacgccggg aggcggcgct ggcaagcagc tgacctgcac tgcagttcat
     2821 gtcgttggcc agcgcccata ccggttttat gtctcgcaca cgggcgatga tgtcagcaca
     2881 gtcaaatgct cccgccacca tcccgcccgg tgtgtccata tcgagcagaa tgccgtccac
     2941 catcggatcg ctggcagcct gttgcagacg ggcgataatg ccgttgtaac cggtcattcc
     3001 cgaatacggc tgcagcgccc gcgtccggct gaccagcgta ccggacaccg gcagcacggc
     3061 gatgccgttc atgacctgat aactccgggc ctgtcgtggt
//
LOCUS       DAZFDC010000266         3098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFDC010000266 DAZFDC010000000
VERSION     DAZFDC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.268"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(138..214)
                     /locus_tag="ACHJ48_004877"
     tRNA            complement(138..214)
                     /locus_tag="ACHJ48_004877"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Arg,seq:tct)
     gene            complement(228..304)
                     /locus_tag="ACHJ48_004878"
     tRNA            complement(228..304)
                     /locus_tag="ACHJ48_004878"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Arg,seq:tcg)
     gene            complement(314..389)
                     /locus_tag="ACHJ48_004879"
     tRNA            complement(314..389)
                     /locus_tag="ACHJ48_004879"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(354..356),aa:Ile,seq:cat)
     gene            complement(430..555)
                     /locus_tag="ACHJ48_004880"
                     /pseudo
     CDS             complement(430..555)
                     /locus_tag="ACHJ48_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(831..1265)
                     /locus_tag="ACHJ48_004881"
     CDS             complement(831..1265)
                     /locus_tag="ACHJ48_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO4710235.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(1351..1491)
                     /locus_tag="ACHJ48_004882"
     CDS             complement(1351..1491)
                     /locus_tag="ACHJ48_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4710236.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1488..1850)
                     /gene="rusA"
                     /locus_tag="ACHJ48_004883"
     CDS             complement(1488..1850)
                     /gene="rusA"
                     /locus_tag="ACHJ48_004883"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO4710237.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(1847..2137)
                     /locus_tag="ACHJ48_004884"
     CDS             complement(1847..2137)
                     /locus_tag="ACHJ48_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO4710238.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2130..2300)
                     /locus_tag="ACHJ48_004885"
     CDS             complement(2130..2300)
                     /locus_tag="ACHJ48_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO4710239.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2300..2755)
                     /gene="ybcN"
                     /locus_tag="ACHJ48_004886"
     CDS             complement(2300..2755)
                     /gene="ybcN"
                     /locus_tag="ACHJ48_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO4710240.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(2752..2853)
                     /locus_tag="ACHJ48_004887"
     CDS             complement(2752..2853)
                     /locus_tag="ACHJ48_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710241.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
BASE COUNT          800 a          798 c          705 g          795 t
ORIGIN      
        1 aaattttttt aacaaacgct cagcatgtca ggcaacattc aactgcctga attgtgaggc
       61 atttaacatt tcactgtccg gtgtctttcc tgtaataaaa agcccgcaaa agagagtcag
      121 ggcagataag tgtggtgtgg cgcgttgtac tggattcgga ccagtgaccg attgcttaga
      181 aggcaattgc tctgtccggc tgagctaaca acgctgaata ccgataatgg accgccatcg
      241 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctgtcccga tgagctaata
      301 gcggtatgta atatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc
      361 gctcgctctc accactgagc taaagggccg ggcgcaggat aataacggta cgtaactaat
      421 cctgcaatat catccgttct gactgactaa atcctgaact tccctgaccg tctgctcaaa
      481 acgttcagtc tccagctcaa cgccaattgc acgacgcccc agcgacattg ctgctttgac
      541 gctacggaca taaaaaagcc agccactggg ggaggctggc aaactcgtag agcaaaatgc
      601 tgttacgcaa acttcgttac agggtcatcc tgcaatacaa aaaatacaca atatttagaa
      661 aactaatagt gccatgtgca atttttaaga ttttgttatt aattgtggtc gcaccttcct
      721 ttctgtgtac tttccgtata gctcacagga ttctgggtac aaaaaaaccc gcgcatcggc
      781 gggttaagca gcgtggcaat gtaaccactc ttatcatgat atgcagattt ttacgatcgt
      841 aaactatttt ttcgctgata aaatacagag gttctccctc ccggcaattc acgctcaaca
      901 taccgatcca tctcaagcct cactcccagc atcatcagca tgccttcaac aatcccctcc
      961 gctttgtgaa ggcgtttacc tatacaggtg tcagagcacc catgtttccg tgccagcgcc
     1021 atgaacgtct cccccaacac gtaataatca accagcaagt catgcagatc gcgattgttc
     1081 cggtaaaggc gggctataca cccgcatatc accatcgcat catcgtcaca gcactgcggg
     1141 cgtgatttta ctttttcggg gatcagtccc ttaaaaccgg cggcaatgga cgaccaggtc
     1201 acatcttcat gattattagc cgcccacgct ccccaacgct caagaaccat ctgaatatca
     1261 cgcatcaact tactccacaa aaatcagacc agaacgccaa ttacaagcaa aaatcaacaa
     1321 aacagtatta gttgattgtt atctctgact tcatactcct gctcctgtca gggttttggc
     1381 gtaattcttc agtattcggt aatcggtcaa aacagaaccg gggaaacgat ataagcgcag
     1441 acgcccccag cggtggcgaa gaagttctgc catattaaac tcaaacatca ttcattcccc
     1501 atttcggtga tggtcagttc cagcctccca cctttggtaa caggcatctt cacaacgcgg
     1561 taatcaacga cctgagcatc atccagccag aaacctgctt tagtgagtgc gtcaaaagcg
     1621 gctttttgca gattatccag gtcacggcga cggcgatccg gcatgtggca ctcaatgcgg
     1681 attttcacag gcatagccag gccgatatcc agcattgcgt ttttaatgat tcgggcgacg
     1741 ttatcgcggt atgcctgccc ctctgcgctg acgtgcgtgc gcccgcgatt atggcggtaa
     1801 tagcgattat tgctcggagg ccagggtaat gtgatgctgt aggtattcac gccttgatta
     1861 ccccctcttt cagccagata acctgcgttc tcgccatacc ttccagcgcg cattcttttg
     1921 cgtactcagc atcgacaaaa tgcgtgcggc ggtcgatttc gtcgtgacag gcagaacagg
     1981 caatggtggc aataaggtct ggcggtttaa taccggtgcc acacagtcca gccagccgga
     2041 tatgtgccag tacagacgtt tcagggttgc cattgcatac gccagggatt cttacctggc
     2101 attcgcgacc acgcgctgct tttctcaaat cagccatgat tcctccttgc tgccagtcgc
     2161 aaccattttt tatcaaccag gctggcggta tatccgagca gtgttggtat ttcggatggt
     2221 tttagctcag gcttacgctt acgacgattt ggtactctgt agatgtgtcc gttcatgaca
     2281 cgaataagcg gtgtagccat tacgcctcct gcttgtcgcg cagcagctgg aactcgcagc
     2341 tctgcggaat agtcaggtgg cagccaatat tcatcgccca ggcttcaacc ttacacagga
     2401 agacatacat ctctccggta tcaagatcgg aggtatggcg taacgactgg atagtagtga
     2461 tttcgccggt tacgacatca accaggtcct tggtttcata accgaggtat gtgtgtttga
     2521 gagcatcttt tacccatgct gcggtagcga acgatttccc cctgcggata aggtattcac
     2581 tgatttcgct gtaccacatg tggctaagtg cattctggga aagactgcgt ctctcgcgcc
     2641 acggtttaag caccatgcga aagcacttgc cgtcttccaa ataaggctgg agctgctgac
     2701 cgatagcggt gaagttaccg cgatgcagtt tgatgccgtc ttgtgggagg ttcacgcttc
     2761 acctccgcag aggtcaaacg ctggataaag aaaatcgcag gtgcatttct gcatctgtga
     2821 cgggagaaga tagtttagat tgtatgtgcg cataaacgtc cccgtttagc gcagaagtca
     2881 ccggaggagt tcaaactccg gtgacttaat tatgacaagt tgattattga aaatcaaaag
     2941 tcatttaaac aataatgata cccagtaaga gatttcttat tttaactcat agatcagaca
     3001 gtcaacgatt tctaaaccat ttaccataga attagaaacg cgaactttac catcgttgac
     3061 tctagcaaat ccttttaaca ttttataaac aaaagctg
//
LOCUS       DAZFDC010000267         3081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFDC010000267 DAZFDC010000000
VERSION     DAZFDC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.269"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            48..842
                     /gene="hyxR"
                     /locus_tag="ACHJ48_004888"
                     /pseudo
     CDS             48..842
                     /gene="hyxR"
                     /locus_tag="ACHJ48_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
     gene            996..1757
                     /gene="hyxA"
                     /locus_tag="ACHJ48_004889"
     CDS             996..1757
                     /gene="hyxA"
                     /locus_tag="ACHJ48_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFO4710242.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIGTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANSSGGLSGLKHAENLPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQVLSLHKYK"
     gene            1799..2881
                     /locus_tag="ACHJ48_004890"
                     /pseudo
     CDS             1799..2881
                     /locus_tag="ACHJ48_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2871..3059
                     /locus_tag="ACHJ48_004891"
     CDS             2871..3059
                     /locus_tag="ACHJ48_004891"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4710243.1"
                     /translation="MLWLRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTK
                     GDDIENPLQGVVGINVTW"
BASE COUNT          852 a          648 c          781 g          800 t
ORIGIN      
        1 ttgaatcaaa atgtcaggtt ttgtagctat ggcgtggacg agtctgaatg aagaagcctc
       61 tggttattat cagtgcctgt cagtttacca ggctggcact ggagtctctc atcccagcgg
      121 acaggtatat cgtgcgggtg tacagcaacg tcactacaga ggttgaattc gttttaaaga
      181 cctcctgtgg ctatctgttg gctgatatcc cttagcttcc ttcgggggag ttagttcttc
      241 tggcttgtct tgtccgcttg cggagtgatg ccgggcaatg gcaggtctgc ctgaccggtg
      301 actccgactg gttcgataat gctcctgctg caagtctgta taacggcttc ccacgcatcg
      361 gcaccatgct gacagcgagt cgttatcaga atcaaattat ccgctggtta ctacgtcctt
      421 tgcccggcga tatgagcgat ttctggctcc tgactgcacg ggagttgagc gtgcttaaga
      481 ttctgatgca gggcatgagt ttcagtgaga ttgcccgtta tgaacaacgc agttcaaaga
      541 ccctgcatgc gattgccaca cgggcattaa tgaaacttgg actgggaact ctgagcgatt
      601 tcaggctgct ctatacggga tgcggtgaca gcagagtgaa acggaatata cggctacatg
      661 caagatatgt cggacaacat tccgctcatc gactcactca gtatgtgcag aacatggtat
      721 ccggaattct ttatccagag aaagttgccg ttggtgaatc aataaattct gacgccattt
      781 ccggaaaagt acttcccgga caaagaaaaa atatttctaa aaaaagacca ggattgttat
      841 gacacaacag cgttgttact accccccact gcggcgcgta cggactcgct acagtgtagg
      901 ggccaaaata tttctactgc tctgggctgc aggattggga ggcacgctgt acagcctgta
      961 cagcgtgctg gcgtactgac atctgtgagg gaaggatgcg tttcgttctt ttctgtccct
     1021 caggaattgt accagcgcag ttcgcagctc tatcaaccgg ttctgtcggc aatgttacct
     1081 gcatcggaac agaagaggag ttacgcaata aattgcggca caggccacag agtgtcgtga
     1141 tatcggcagg gcgacctgcc gaatgcgctg aaatgtggtt ccgattttat cgggatcact
     1201 cattcgtggt ggtgttgtgc gtcgcgccat tttttctgcc accggatgtg agtatttctg
     1261 gcgttctcaa aaacctgagg ttactgaaac caggaatgtc agtagagcat gtcatcagta
     1321 ttgcaaattc ctctggtggg ttatcagggc tgaaacatgc agaaaatttg ccagttatgg
     1381 acagttactc tgtctttatg aaagaggtga ataaccgcac aaagacaatc gtcatgtcag
     1441 aacgcttccc ggaaaagcaa aagaaggtgt tgtcgttgct tcttgccgga cacagctggg
     1501 aatacagtgc gcagtttctc aaaacgggga taagacagat atggcttgcc gagcagtcgc
     1561 taaaaaagcg ttggggaata ccggacagca tgtcattgcg ggaagccctg ctgctgagca
     1621 gcaataaatt tcacggtgat ggaaatgcat tggaaaaaac gaatgccatg acattgcgtg
     1681 aaaacggaaa taccaacagg tattccgtgg tggtaaatgc agggacgcag gttttatctc
     1741 tgcataaata taaataagtg ggaaattgaa attagttacc agaatggaga atttcttcat
     1801 gaaaaacagt aaggcatttt accgcagcgc attagcgaca gctattgtta tggctctttc
     1861 tgcaccagca ttcgctgctg ataacgcggt atcaactgat tcggttacgc tgaataaaga
     1921 taagacgact ctggatcaag atgttgttat tagcaataca gcagacaaac agattacagc
     1981 cgtaacaatt aatgcggcag atgaagacct taatgttgct tttgctggtc atgatattac
     2041 tgctgaatca acggcagaca aaaaattcgt tgaaggtgta aaagttagtg gtaacaaaaa
     2101 tgttgtgatt aatgctacag actccaccat cacagctcaa ggtgaaggca cctatgtccg
     2161 gactgcaatg gtcattgatt caactggcga tgttgttgtt aatggcggta atttcgttgc
     2221 aaaaaatgaa aaaggtagtg cgacagggat atctctggaa ggggccaagg gaaataatgt
     2281 cacgcttaat ggcacaacca taaatgctca aggtaataag agttccagca acgcctccac
     2341 ggcaattttt gctcaaaagg gtagcttatt gaatagtttt gaaggtgatg caaccgacaa
     2401 cattaccctt gctggctcaa atattattaa tggtcggatt gaaacaatag ttactgccgg
     2461 gaataagacg ggaattcata cagtcaacct gaatattaag gatggctcag taattggggc
     2521 ggctaataat aaacaaacaa tttatgcctc tgcttcggca caaggcacag gttcagcaac
     2581 gcaaaattta aatttgtctg ttgctgattc aaccatctac tctgatgtcc tggccctttc
     2641 tgaaagcgag aattcagccg ctaccacaac aaatgtaaac atgaacgttg cccgctctta
     2701 ctgggaaggt aatgcttata ctttcaatag cggcgataaa gcgggtagta atctggatat
     2761 aaatctttcc gatagttcag tctggaaagg caaagtttca ggggcaggaa atgccagtgt
     2821 atctctgcaa aacgagtctg tctggaatgt tacgggatcc tcaactgttg atgctctggc
     2881 tccgtctggg tgatgagtct gtcaatgcca gcattgacgg tgcagcattc cgcgtgggtg
     2941 caggtgtaca ggctgatatc accaaaaaca tgggagcata tgcaagcctt gactacacca
     3001 aaggtgacga cattgagaat ccgctacagg gtgtagttgg tatcaatgtg acctggtaag
     3061 aataaagcag tcggttcccc t
//
LOCUS       DAZFDC010000268         2945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFDC010000268 DAZFDC010000000
VERSION     DAZFDC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.270"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..1445
                     /locus_tag="ACHJ48_004892"
     CDS             45..1445
                     /locus_tag="ACHJ48_004892"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO4710244.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            1816..2640
                     /gene="gadX"
                     /locus_tag="ACHJ48_004893"
     CDS             1816..2640
                     /gene="gadX"
                     /locus_tag="ACHJ48_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO4710245.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDDHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDCSAEGIRL"
BASE COUNT          782 a          657 c          685 g          821 t
ORIGIN      
        1 atcctacttt tttattgcct tcaaataaat ttaaggagtt cgaaatggac cagaagctgt
       61 taacggattt ccgctcagaa ctactcgatt cacgttttgg cgcaaaggcc atttctacta
      121 tcgcggagtc aaaacgattt ccgctgcacg aaatgcgcga tgatgtcgca ttccagatta
      181 tcaatgatga attatatctt gatggcaacg ctcgtcagaa cctggccact ttctgccaga
      241 cctgggacga cgaaaacgtc cataaattga tggatttgtc gatcaataaa aactggatcg
      301 acaaagaaga atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaac atggttgccg
      361 atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt tggcaccaac accattggtt
      421 cttccgaggc ttgtatgctt ggcgggatgg cgatgaaatg gcgttggcgc aagcgtatgg
      481 aagctgcagg caaaccaacg gataaaccaa acctggtgtg cggtccggtg caaatctgct
      541 ggcataaatt cgctcgctac tgggatgtgg agctgcgcga gatccctatg cgccccggtc
      601 agttgtttat ggacccgaaa cgcatgattg aagcctgcga cgaaaacacc atcggcgtgg
      661 tgccgacttt cggcgtgacc tacaccggta actatgagtt cccgcaaccg ctgcacgatg
      721 cactggataa attccaggcc gacaccggta tcgacatcga catgcacatc gacgctgcca
      781 gcggtggctt cctggcaccg ttcgtcgccc cggatatcgt ctgggacttc cgcctgccgc
      841 gtgtgaaatc gatcagtgct tcaggccata aattcggtct ggctccgctg ggctgcggct
      901 gggtgatctg gcgtgatgaa gaagcgctgc cgcaggaact ggtgttcaac gttgactacc
      961 tcggtggtca gattggtact ttcgccatca acttctcccg cccggcgggt caggtgattg
     1021 cgcagtatta tgaattcctg cgcctcggtc gtgaaggcta taccaaagta cagaacgcct
     1081 cttaccaggt tgccgcttat ctggcggatg aaatcgccaa actggggccg tatgagttca
     1141 tctgtacggg tcgtccggac gaaggcatcc cggcggtttg cttcaaactg aaagagggtg
     1201 aagatccggg atacaccctg tacgacctct ctgaacgtct gcgtctgcgc ggctggcagg
     1261 ttccggcctt cactctcggc ggtgaagcca ccgacatcgt ggtgatgcgc attatgtgtc
     1321 gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa gcctccctga
     1381 aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc tttaaacaca
     1441 cctgataaca taacgttgta aaaaccgaat gcccagcctt taaaaaaaac agctgggcat
     1501 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
     1561 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgac aagggattat
     1621 ttgcttacta ttaatttccc tgcgttctta aacacgtttt tgtattataa atccagtcat
     1681 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
     1741 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat aaacagtaat atgtttatgt
     1801 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
     1861 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
     1921 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
     1981 agggatacgc tttcattatt tctcccgatg ctcaaggagg aggcattaaa tcttcatgca
     2041 cataaaaaag tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
     2101 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
     2161 gcattaatct ttgcgttgtt atctgttttt cttgaggatg atcactttat accgctgctt
     2221 ctgaacgttt tacaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
     2281 gctcatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagtctgtta
     2341 aagaaaaaat tacgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
     2401 caacgtgctt tgcaacttat tgttatacat ggtttttcaa ttaagcgagt cgcagtgtcc
     2461 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     2521 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     2581 attgttgcgc aagggaattt ttacggcact gactgttctg cggaaggaat aagattatag
     2641 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     2701 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     2761 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     2821 atacgctgtg cgaaaatata ttcgtgttgc atttacttat tatcaattaa ctgttatgca
     2881 aaactacttt gtggataaat tttggtccta ccaaatttgg cagtttttgc gctaagaaac
     2941 agtct
//
LOCUS       DAZFDC010000269         2882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFDC010000269 DAZFDC010000000
VERSION     DAZFDC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.271"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1890
                     /locus_tag="ACHJ48_004894"
     CDS             <1..1890
                     /locus_tag="ACHJ48_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO4710246.1"
                     /translation="LAEKLTHKLKEGWQPFGSPVAITPYTLMQAIAAEGDVTTPVVVP
                     GTGDGGYPGVVTTEPDYYYVIPLAGQSNGMAYGEGLPLPQTYDRPDPRIKQLARRSTV
                     TPDGAPCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIP
                     ANAGILLVPCCRGGSAFTTGADGTYSDVTGASESSTRWGVGRPLYKDLIGRTKAALAK
                     NPKNVLLAVVWMQGEFDFDGTPANHTARFTEVVEQYRTDLADMVGQCAGGSADGVPWI
                     CGDTTYFWKQKSESTYQTVYGSYKNKTEKNIHFVPFMTDENGANVPTNKPEEDPDIPA
                     SGYYGAASRTSANWTSADRASHFSSWARRGIISDRLASAILLHAGRTAELVGGEQVVM
                     PPDEKPSPDTPSTPSTDGKSVTTLLYYRATESGGLLNPQGWGAEGGRALVVDDAGAAG
                     GKALRWTKQTGSSSWFMQHDAGNGADLLEKGGLISCRFKVDGTLTANQYALALYWPVS
                     SLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNAGQNTKLGSFGAFDNEWHTLG
                     FRFAGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITSGATYPVLIESITV
                     EVNNP"
     gene            2038..2220
                     /locus_tag="ACHJ48_004895"
     CDS             2038..2220
                     /locus_tag="ACHJ48_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO4710247.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            2258..2527
                     /locus_tag="ACHJ48_004896"
     CDS             2258..2527
                     /locus_tag="ACHJ48_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4710248.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPGGEMMM"
     gene            2603..2818
                     /locus_tag="ACHJ48_004897"
     CDS             2603..2818
                     /locus_tag="ACHJ48_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4710249.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2823..>2882
                     /locus_tag="ACHJ48_004898"
     CDS             2823..>2882
                     /locus_tag="ACHJ48_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO4710250.1"
                     /translation="MKKKYELVVKGINNYPDKIT"
BASE COUNT          728 a          668 c          862 g          624 t
ORIGIN      
        1 cttgcggaaa agctgacaca caaactgaaa gagggctggc agccgtttgg tagtcctgtg
       61 gccataaccc cttataccct gatgcaggcg attgccgcgg agggggatgt gaccacgcca
      121 gtggttgtgc ccggcacggg ggatggtggc tatccgggag tggtcaccac ggagccagat
      181 tattactacg ttattccact ggccgggcag tcgaacggca tggcttacgg tgaggggctt
      241 cctctgccgc agacatatga ccgtcctgac ccgcgtataa agcaactggc tcgtcgcagc
      301 acagtgacgc cggatggcgc tccctgtaaa tataacgaca ttattccggc agaccactgt
      361 ctgcatgatg tacaggacat gagccgtctt aaccatccga aagctgacct gtcgaaaggt
      421 cagtacggaa ccgtggggca ggggctgcat attgccaaaa agctgctgcc gtttataccg
      481 gcgaatgcgg gtattcttct ggttccgtgc tgccgtggtg gttcagcttt caccaccggg
      541 gcagatggaa catacagtga cgtgaccggt gcctcagaga gttctacccg ctggggtgtg
      601 ggcaggccgc tgtataagga tctcatcggt cgtacaaaag ccgcgctggc aaagaacccg
      661 aaaaatgtgc tgcttgccgt ggtgtggatg cagggggaat ttgactttga cggaacgcca
      721 gcaaatcaca cagcccgttt tacagaagta gtggaacaat atcgtacgga ccttgcagat
      781 atggtgggac agtgcgctgg tggttctgct gacggtgttc cctggatatg tggagacaca
      841 acttatttct ggaagcagaa gagcgaatcc acttaccaga cggtgtacgg cagttacaaa
      901 aacaaaacgg aaaagaatat tcactttgtg ccgttcatga ccgatgagaa cggagcaaat
      961 gtcccgacga acaaaccgga agaagacccg gatattccgg catcaggata ttacggtgcg
     1021 gcctcccgga cgtcggcaaa ctggacgtca gcagaccgtg cgagccattt cagctcatgg
     1081 gcacgcaggg ggattatttc tgaccgtctt gcctcagcga ttcttctcca tgcaggacgg
     1141 acggctgaac tggtgggtgg ggaacaggtt gtgatgccgc cggatgagaa gccgtcaccg
     1201 gacacaccat caacaccgtc aacggacggg aaatcagtga caacgctgct ttattaccgt
     1261 gcaacagagt caggtggttt actgaatccg cagggatggg gagctgaagg agggcgtgca
     1321 ttggtagttg atgatgcagg tgctgcagga ggtaaggcgc tgaggtggac caaacagaca
     1381 ggaagttcct cgtggtttat gcagcatgat gccggtaatg gcgcagacct gctggagaag
     1441 ggcgggctta tcagttgtcg ttttaaagtt gatggcacac tgacagctaa tcagtacgca
     1501 ctggcgctgt actggccggt ttcttcactg cctcagggcg tcacactgga aggtaatgcc
     1561 ggtcataacc tgctggcgtc gttttacgta cagagcgatg ccacagacct taatgtgatg
     1621 taccacaagg gaaatgctgg tcagaacacg aagctggggt cattcggcgc atttgataac
     1681 gaatggcata cgctgggctt ccgttttgcc ggtaacaaca gtattgaggt gacgccggtc
     1741 attgatggta aggacgggac gccgttcatg ctgtcacagt caccggtcgg cacgtttacg
     1801 gcagacaaat tgcgcgtgac cgatatcact agcggtgcga catatccggt gctgattgaa
     1861 agtataacag tggaagtgaa taacccgtaa gcaggaaaaa aaggccgccg gggcagggaa
     1921 aacaaggagc cagaaccggc ggcaaatgtc gttatatcca aagcaaaaca tgcaggacac
     1981 ttttttaacc aacaggtatt aacgatgtca acaccatatc aataaccggg agggataatg
     2041 agatttgtac agcttatttt attgtatttc tgcacggtgg tgtgcacgtt atatctggta
     2101 agtggcgggt ataaggttat ccggaactat atacgcaaaa agattgatgc cgcggcggcg
     2161 gaaaaaatca gcgccagcca gtcagccgga acaaaacccg aagagcctct catttcgtag
     2221 caactttctt aacaacacct ttcaacgaga aaatcccatg tcagaaataa aatctctggt
     2281 cactgctgaa gcagtgaagg acgtcctgcg ctctgaagaa gtcagaagcg cactgaaaca
     2341 gcaactccgc cagaatcttg aggcgcgtct tgatgctgaa gtggatgcca ttctggatga
     2401 gctgctgggg ggaccggctg ctcctgagcc tgaagacggc gcgggtgaca gtgctgtttc
     2461 agatggcgtt gtgtctcagc ctgacggtag cagtgagcct cagcctggcg gcgaaatgat
     2521 gatgtaacca tacgcagggg ctgtcggtgt gagctgatgc ccctcctgtt gttgtgagct
     2581 tccggattgc gggagacggg gtatgtacca gatggaaaaa atcacaacgg gtgtgtcata
     2641 caccacgtca gcggtgggga cgggatactg gttactgcag ctgctggaca aagtctctcc
     2701 gtcccagtgg gtggcgatag gtgtgctggg gagtctgctg tttggcctgc tgacgtatct
     2761 gactaacctg tatttcaaaa tcagagagga ccgtcgtaag gcggcgcggg gagagtaaag
     2821 cgatgaagaa aaaatacgaa ctggttgtta aagggataaa taattacccg gataagatta
     2881 ct
//
LOCUS       DAZFDC010000270         2873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFDC010000270 DAZFDC010000000
VERSION     DAZFDC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.272"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(106..182)
                     /locus_tag="ACHJ48_004899"
     tRNA            complement(106..182)
                     /locus_tag="ACHJ48_004899"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Asp,seq:gtc)
     gene            complement(315..1046)
                     /gene="dnaQ"
                     /locus_tag="ACHJ48_004900"
     CDS             complement(315..1046)
                     /gene="dnaQ"
                     /locus_tag="ACHJ48_004900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO4710251.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRLVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1111..1578
                     /gene="rnhA"
                     /locus_tag="ACHJ48_004901"
     CDS             1111..1578
                     /gene="rnhA"
                     /locus_tag="ACHJ48_004901"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO4710252.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1575..2297)
                     /locus_tag="ACHJ48_004902"
     CDS             complement(1575..2297)
                     /locus_tag="ACHJ48_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4710253.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFY
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2331..>2873
                     /gene="gloB"
                     /locus_tag="ACHJ48_004903"
     CDS             2331..>2873
                     /gene="gloB"
                     /locus_tag="ACHJ48_004903"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO4710254.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPH"
BASE COUNT          761 a          701 c          689 g          722 t
ORIGIN      
        1 ccagagtaaa atcattgaac agagaaatgg tggaattcaa atacaaaaaa aaccgcaaaa
       61 ttaaaaatct tgcggttctc tgaactcatt ttcatgaggt gaatttggcg gaacggacgg
      121 gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct
      181 ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcacc cttcgtcaac
      241 ggtttttctc atcttttgaa tcatttgctg caaaaatcgc ccaagtcgct atttttagcg
      301 ccttttacat gtatttatgc ccgccagagg cagcttccgc cctttttctg caccagatcg
      361 agacgggctt catgtgctgc aagctcttca tctgtcgcaa aaacaacgcg taacttactt
      421 gcctgacgta caaggcgctg gattgttgct tcaccttgtt gctgttgtgt ctctccttcc
      481 atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc cgcaaggatc
      541 tgggcatcga gtaatgcccc gtgcagcgtt cgtttactgt tatctatttc gtagcgggca
      601 cataacgcat cgaggctgtt gcgtttaccg ggaaacattt tcctcgccac cgcaaggcta
      661 tcggtgacct tacagaaggt attagtcttc ggaatatcgc gcttaagcaa cgagaactcg
      721 tagtccataa agccgatatc gaacgctgcg ttatggatca ccaactccgc gccgcggata
      781 tagtccatga actcatcggc tacttcggca aacgtgggct tatcgagcaa aaattcatcg
      841 gcaataccat gtacgccaaa ggcttccgga tccaccagcc gatcgggttt gagataaaca
      901 tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat ctcaatgatc
      961 ttgtggcctt catagtgcgc accaatctgg ttcataccgg tggtttcggt atcgagaacg
     1021 atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttgt cgttttacag
     1081 ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat tttcaccgat
     1141 ggttcgtgtc tgggtaatcc tggacctggg ggttacggcg ctattttacg ctatcgcgga
     1201 cgcgagaaaa cctttagcgc aggctacacc cgcaccacca acaaccgtat ggagttgatg
     1261 gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt gagtaccgac
     1321 agccagtatg tccgccaggg tatcacccag tggatccata actggaaaaa acgtggctgg
     1381 aaaaccgcag acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct tgatgctgca
     1441 ttggggcagc atcaaatcaa atgggaatgg gttaaaggcc atgccggaca cccggaaaac
     1501 gaacgctgtg atgaactggc tcgtgccgcg gcgatgaatc ccacactgga agatacgggc
     1561 taccaagtcg aagtttaagc ctgtggttta cgacattgcc gggtggctcc aaccgcctgg
     1621 cgaattcgtg gcttgttttt actctgtttc atcggattta gcgttaaagg aatagtccgt
     1681 ttccgggcaa caataagttg taagcaacca agcgcaggaa tatgcgcatt caaaagtttt
     1741 cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgtagcac ttcaaaattc
     1801 aacaaagaga gccagtccag ttgccgcatc agagtaaaca tccggctgtt atagggcgag
     1861 gttttgcgca ataccggcac aagtttgcgt aatcccatta aactaatggg attgaagcca
     1921 ctaatgacca gccagccatc atcaatcaat acccgatcgg cttcacgcaa taaacgatgc
     1981 ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa catcaacgga tttatcggca
     2041 aaaggaagat gaagtgggtc cgcctgcacc tggacaggca ttccttgcgc agaaacattc
     2101 acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt gccaatctta
     2161 agcagataaa aaccatacat tttagtgaac cacgggttga gctggcgctc cagcgccttg
     2221 cgataaagct ctccccaggg caaatcgccc cagcgatcag gagccacgac agtttgaggg
     2281 actcttgccg gtttcatcac aaccttccgt ttcacactga gaggtaatct atgaatctta
     2341 acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa gcaggtcgct
     2401 gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc gccaataact
     2461 ggcaaccgga ggccatattt ctcacccacc atcatcacga tcacgttggc ggcgtaaaag
     2521 aactggtgga aaagtttccg caaattgtgg tgtatggtcc acaagagaca caagataagg
     2581 gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat gaatttagtg
     2641 taattgccac gccgggtcac actttaggac atatctgtta cttcagtaaa ccttatctat
     2701 tttgcggcga cacgctgttt tctggtgggt gtggtcggtt gtttgaaggg acagcatcac
     2761 aaatgtatca atcacttaaa aagttaagtg cgttacctga cgatacattg gtatgttgtg
     2821 ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg cac
//
LOCUS       DAZFDC010000271         2846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFDC010000271 DAZFDC010000000
VERSION     DAZFDC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.273"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..254
                     /locus_tag="ACHJ48_004904"
     CDS             3..254
                     /locus_tag="ACHJ48_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4710255.1"
                     /translation="MMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENGIIPEQD
                     RVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            368..943
                     /locus_tag="ACHJ48_004905"
     CDS             368..943
                     /locus_tag="ACHJ48_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4710256.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
     gene            1654..2304
                     /gene="nleG7"
                     /locus_tag="ACHJ48_004906"
     CDS             1654..2304
                     /gene="nleG7"
                     /locus_tag="ACHJ48_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO4710257.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQKQHCK"
     gene            2454..>2846
                     /locus_tag="ACHJ48_004907"
     CDS             2454..>2846
                     /locus_tag="ACHJ48_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFO4710258.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMS
                     HHLGYDKNQPKPGANSRNGYSTKTVITGDGHLELRTPRDRDGSFEPQLVKKNQTRITG
                     MDNQILSLYAKGLTTREIAAAFKELYDAD"
BASE COUNT          870 a          551 c          641 g          784 t
ORIGIN      
        1 aaattatgca gcgtctgtgc ggttgcggaa agcatgatga ccgtgaaaac ggggagttac
       61 ttacagcaca gctgcgactg ggaccggcag acattctgga gtccgatgag aatggtatta
      121 tcccggagca ggacagggta atcacgcagg tggtgatact ggatgcggat aaaaagcaga
      181 tacagtgcgt ggtaagaccg ctgcaaatcc tgcgtgctga cgggacgtgg gaaaatattg
      241 gcgggatgaa atagcccgac agcttcacaa aaaccggagt ccggctccgg tttttgttgt
      301 catgtatggg ggctgtttgt tatgactccc tgtgtttgga atgaatattt aaatagggag
      361 ttttgtcatg ccgttaacat cagctattgc atccaattca ttttccaccg gaatgcaggt
      421 tcttcgtgct caaatggccg ccagtggcgg tggagaaatt acagtaggcg ggcagacggt
      481 ccgtatcaca tatagtgaaa cggatggtcg ctttctggcg agtgggggca ataacagttt
      541 gctttctgga ttattactta cagggcttaa tggtggtcct gaagcgctca gggatataat
      601 gttaagaatg gtttcaggtt caggtaacac acaatcacat ggtgatattg aggggaaaat
      661 atcacaatgt aagttttctg ttaatacgga gagccttcag tgtccatccg aggcggttcg
      721 atgcccaatt atactggata aaccagaaga aggtgtgttt gttaaaaatt cagaaggttc
      781 tttggtttgt accttatttg attcggtttc tttttctcat ttggttcgcg acggtgggaa
      841 gcacccgcta acacgagaac caataacgtc atcaatgatt gtaagtcaag aacaatgtat
      901 ttatgaccaa accaaaggaa actttgtcat aaaggataag tgaaataaat attacccaag
      961 ctatatgtta actgccagtt gcttatatga aatgctacag atgttcaggg tataaggatg
     1021 tggtaattgg tgtactggat gcagctaaaa agcatataca gtgagtggtg aggccgctgc
     1081 aaatctggcg tgctgacggg gaaatattgg cgggatgaaa tagccgacgg attcacaaaa
     1141 accggagtcc ggctccggtt tttgttgtca tgtcagggag atgtttgtta tgaagcccag
     1201 aggaaatatt tatctgtatg aaggaatatg gtaatgcctg gattagtatc atatatatca
     1261 tcgacttcat tcgcgaatga gatggcggag atgcgtcagc aggtaatgga agggcagatt
     1321 ggtggatttc tcctgggagg ggagagagtt agagtttctt atttatttca attgcattaa
     1381 tatcttgaat ggattacata gagttaatgt atccagtact ccctattctc ttgcatataa
     1441 tacatgttgc aacttacatc tcagcgctat gaaaaaacac cacctctctc tttatgaaat
     1501 tctggattta ccaagcgcta atttatcatt tcaatctacc tttaagtatt gcatttatct
     1561 ccctacgagg tcatacttta ggaagttaaa tatgaatgat aatataccta cagcgcgaaa
     1621 tcacaaacaa tcgacttgta ttacagaaaa aacatgccta tatttttaaa cttcacagca
     1681 ggaagtatct tacctgagaa tgagctagca tctttacgtt atattgtgca gcaaaatcaa
     1741 aatgatactg taatcataaa agaacgttat aaaatggata tccgttatat cgaatcagtc
     1801 aatggtttta cagtaaatcc tgtatgcagt aatcatttct ccatatttat ggcgagacaa
     1861 aacactattg ctcgcaacct ggaacagcag atcaacaacg gacgaagttt tgcacaaata
     1921 tctcaggatt ttatgcttca attatcttca aatataggat ggaaaaaagg ggccgaaaac
     1981 gcccttaaaa ataaaatcca ttctcattca tttgttgtaa atcctgatga attctcttgc
     2041 gacacacaat ttcttaagtg cccaataaca ttatgcgttc cagaaaaagg ggtttttgtc
     2101 aagaatgcac tgaactccaa catatgcact ctttatgata agtctgcgtt catgaatctc
     2161 acaagagaac atctacccca ccctctcagc agggaaaaga tagtaaaaga aatgattatt
     2221 gaaaggaata tgtgttattt tgacaccata agtcagcatt tcataattat ggatgcagac
     2281 caacagaaac agcattgtaa ataaaatgta ataattacat actattagtg attctcatgc
     2341 atcgtaagcg gctcgccaga accgtattga tatttactga ggagcctgta catagatttg
     2401 tgtaattgcc tgattttgat atgttcaatc caacatcaaa agcaggttaa tttatggacg
     2461 aaaaacagtt gcaggctctg gctaacgaac tggccaaaaa tctcaaaacc cctgaagatc
     2521 tcagccagtt cgatcgcctg ctgaagaaaa tcagcgttga ggcagctctc aacgctgaaa
     2581 tgtcccacca tctgggctac gataaaaacc agcctaaacc gggtgccaac tcccgcaatg
     2641 gctattccac aaagaccgtt atcaccggtg atggccacct ggaactacgc actccgcgcg
     2701 atcgtgatgg ctcttttgaa ccgcaacttg tgaagaaaaa ccagacccgg atcaccggga
     2761 tggataacca gatcctgtcg ttgtacgcca aaggcctgac aacccgcgaa atagcggctg
     2821 cgtttaaaga gctgtatgac gctgat
//
LOCUS       DAZFDC010000272         2801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFDC010000272 DAZFDC010000000
VERSION     DAZFDC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.274"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..570
                     /locus_tag="ACHJ48_004908"
     CDS             <1..570
                     /locus_tag="ACHJ48_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4710259.1"
                     /translation="KTGAEAIRALATQLPVFRQKLSDGWYQVRITGRDVSTSGLTAQL
                     HETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMT
                     GILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYG
                     EMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            631..>2801
                     /locus_tag="ACHJ48_004909"
     CDS             631..>2801
                     /locus_tag="ACHJ48_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4710260.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLSSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGASFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTP"
BASE COUNT          603 a          738 c          922 g          538 t
ORIGIN      
        1 aaaacggggg ctgaagccat ccgcgcactg gccacacagc tcccggtgtt tcgtcagaag
       61 ctgagcgacg gctggtatca ggtacggatt accgggcggg acgtcagcac gtccgggtta
      121 acggcgcagt tacatgagac tctgcctgat ggcgctgtga ttcatattgt tcccagagtc
      181 gccggggcca agtcaggtgg cgtattccag attgtcctgg gggctgccgc cattgccgga
      241 tcattcttta ccgccggagc cacccttgca gcatgggggg cagccattgg ggccggtggt
      301 atgaccggca tcctgttttc tctcggtgcc agtatggtgc tcggtggtgt ggcgcagatg
      361 ctggcaccga aagccagaac tccccgtaca cagacaacgg ataacggcaa acagaacacc
      421 tatttctcct cactggataa catggttgcc cagggcaatg ttctgccggt tctgtacggt
      481 gaaatgcgcg tggggtcacg cgtggtttct caagagatca gcacggcaga cgaaggggac
      541 ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctccg
      601 ggcggtttta tcgtttatgg agcatgacga atgggtaaag gcagcagtaa ggggcatacc
      661 ccgcgcgaag cgaaggacaa cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc
      721 agcgaagggc cggttgaagg tccggtggat ggattaaaaa gcgtgctgct gagcagtacg
      781 ccggtgctgg acagtgaggg gaataccaat atatccggcg tcacggtggt gttccgggcc
      841 ggtgagcagg agcagacacc gccggaggga tttgaatcct ccggctccga gacggtgctc
      901 ggtacagaag tgaaatatga cacgccgatc acccggacca tcacgtcggc aaacattgac
      961 cgtctgcgtt ttactttcgg cgtgcaggca ctggtggaaa ccacctcaaa gggggacagg
     1021 aatccatcgg aagtccgcct gctggttcag atacaacgta acggtggctg ggtgacggaa
     1081 aaagacatca ccattaaggg taaaaccact tcacagtatc tggcctcggt ggtggtggat
     1141 aacctgccgc cgcgcccgtt taatatccgg atgcgcagga tgacgccgga cagcaccaca
     1201 gaccagctgc agaacaaaac gctctggtcg tcatacaccg aaattatcga tgtgaaacag
     1261 tgctacccga acacggcact ggtcggcgtg caggtggact cggagcagtt cggcagccag
     1321 aaggtgagcc gtaattatca tctgcgcggg cgtattctgc aggtgccgtc gaattataac
     1381 ccgcagacgc ggcaatacag cggtatctgg gacggaacgt ttaaaccggc atacagcaac
     1441 aacatggcct ggtgtctgtg ggatatgctg acccatccgc gctacggcat ggggaaacgt
     1501 cttggtgcgg cggatgtgga taaatgggcg ctgtatgtca tcggccagta ctgcgaccag
     1561 tcagtgccgg acggctttgg cggcacggag ccgcgcatca cctgtaatgc ctacctgacc
     1621 acacagcgta aggcgtggga tgttctcagc gatttctgct cggcgatgcg ctgtatgccg
     1681 gtatggaacg ggcagacgct gacgttcgtg caggaccgac cgtcggataa ggtgtggacc
     1741 tataaccgca gtaatgtggt gatgccggat gatggcgcgt cgttccgcta cagcttcagc
     1801 gccctcaagg accgccataa tgccgttgag gtgaactgga ttgacccgga taacggctgg
     1861 gagacggcga cagagcttgt ggaggacacg caggccattc tccgttacgg tcgtaacgtc
     1921 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
     1981 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtggg cgcagaaggg
     2041 cttcgccatg taccgggcga tgtcattgaa atctgtgatg atgactatgc ggggatcagc
     2101 atcggtgggc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
     2161 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
     2221 gtcagcgtgg aggtccagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
     2281 gacggcgttg ccggatacag cgtatggggg ctgaagttgc cgacgttgcg ccagcgcctg
     2341 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
     2401 catgtacccg aaaaagaagc catcgtggat aacggggcgc actttgacgg cgacctgagc
     2461 ggcacggtga atggcgtcac gccgcccgcg gtgcagcacc tgactgccga agtcaccgca
     2521 gacagcgggg aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggggtg
     2581 agcttcctgc ttcgcctgac cgtggcagcg gacgatggca gtgagcggct ggtcagtacg
     2641 gccaggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
     2701 actgtccggg cggtaaatgc gtggggacag cagggcgatc cggcatcggt atcgttccgg
     2761 attgccgcac cggcagcgcc gtctcggatt gagctgacac c
//
LOCUS       DAZFDC010000273         2797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFDC010000273 DAZFDC010000000
VERSION     DAZFDC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.275"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            29..787
                     /gene="paa"
                     /locus_tag="ACHJ48_004910"
     CDS             29..787
                     /gene="paa"
                     /locus_tag="ACHJ48_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO4710261.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1123..1278
                     /gene="hokD"
                     /locus_tag="ACHJ48_004911"
     CDS             1123..1278
                     /gene="hokD"
                     /locus_tag="ACHJ48_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4710262.1"
                     /translation="MKQQKAMLIALIVICLIVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1499..1756
                     /locus_tag="ACHJ48_004912"
     CDS             1499..1756
                     /locus_tag="ACHJ48_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710263.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1826..2104
                     /locus_tag="ACHJ48_004913"
     CDS             1826..2104
                     /locus_tag="ACHJ48_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710264.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2106..>2797
                     /locus_tag="ACHJ48_004914"
     CDS             2106..>2797
                     /locus_tag="ACHJ48_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4710265.1"
                     /translation="MRVLLRPVPVPELGVVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQ"
BASE COUNT          735 a          654 c          738 g          670 t
ORIGIN      
        1 tcatgataac caataaaagg agttttttat gaggaacata atggcaggtt ttttaatatt
       61 cctgtcttct gctgcttatg ctgatatcaa tctgtatggt cctggtggcc cgcatacagc
      121 cttgcttgat gcagccaaac tttatgccga aaaaacaggt attatagtga acgttcatta
      181 cggcccacag aacaaatgga atgaagatgc caaaaaaaat gcagatatct tgtttggcgc
      241 atcagaacaa tctgctctgg ctatcattcg ggaccataaa gacagcttca gtgaaaaaga
      301 tattcagcct ctttatctgc gaaaaagtat tttactggta aagaaaggta atcctaaaaa
      361 tatccggagt attgacgacc tgaccagacc tgggattggc gtaattgtta atgatggtgg
      421 tggtaccagt aatacatcag gcactggcgt ctgggaagat attgccggac gtaaagggaa
      481 tatagaaact gtcgccgcaa tccgaaaaaa tattatttta tatgcgccca atagcggaac
      541 tgcacgtaag gctcttgaga atcagcctgg agcagatgtc tggataacct gggctgactg
      601 ggcagccagt aatccagaaa ttggtgatgt cgtggaaata gcgccagact acgtgatatg
      661 gcgtgatatg aacattacag tacgtcagga tgcaaatgat gaaacccgtc gatttgcaga
      721 atggctacaa accgatgaag cggcgcctgc attcaaaaaa tatggctgga ccaggaaagg
      781 cacttgacat cctcgtcctt caggacgtgg attctttttc cggatgccgc gccagcggca
      841 tgtaggggca gctcacaaaa cggaaaaaat tgtacgctaa gcctcgccag gtgaactgaa
      901 ttcattccga tatgggaatt cccatatcgg gcgaaaacgg tttgctgtaa cggcagagtt
      961 aagtaggatt gctgcgggtg cttgaggcta tctgcctcgg gcatgaacac caacggcaga
     1021 tagataaaag ccccacccga ctataaatcg aagtgaggcc cctatatgct cgtcacatat
     1081 agattgcctc ttacggaccg aaaggtcaag gagaagcagg ctatgaagca gcaaaaggcg
     1141 atgttaatcg ccctgatcgt catctgttta atcgtcatag tgacggcact ggtaacgagg
     1201 aaagacctct gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct
     1261 tacgaacctg aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctgatgtgt
     1321 caggcatcct caacgcaccc gcacttaacc cgcttcggcg ggtttttgtt tttattttca
     1381 acgcgtttga agttccggac ggcgccggaa tagaatcaaa aatacttaag tagcgcgcag
     1441 ggagaagagg gatggacccc gaacagggga gtgctattta tctggaagga ttctgttgat
     1501 gagaatcgaa gaattacgtg aaatttttag tgaagatggc ctctatactg tgcgcgttga
     1561 gaagggcgct attgtcagcc actgccgtat taaatgttta cagtctcaac aaaggaagag
     1621 tggagctgcg ttaattcatt ttgtggatgg gcttgtgacg gatggtttta ttttgcgtgc
     1681 aaatgaattt gtcacatcgt tgccgtctct gaaagaagct gggattaagg ctggtttttc
     1741 tgcttttgaa gattagtgaa ttcatctaca attcagcgca gggctgaacc cctgttgagt
     1801 aacactgtgc caccggagaa agccgatggc gcaaaattcc agactacaca attctgataa
     1861 ttcagccgtc tttgccagca ggcacgggcg gcgttctcat gcattcaaat ctgactggtt
     1921 ccggcacgcc ccatgcactg aagaacaggc cgaatggctg attcagaact accgcagacg
     1981 tgggtatgag tttaggaaag ccctcagcct cgattatcgt cactggataa tctacgtcag
     2041 gctgccgtac tccgagcgcc caccgcgtcc gtcccgcaca ttccagcaac gcatctggag
     2101 gtaacgtgcg ggtattactt cgacctgttc cggtaccgga acttggggtg gtggtcctta
     2161 aaccaggccg tgaatccatg caggtattcc ataaccctcg agtgcttgtg gagccggagc
     2221 cgaaaagcat gcgcggtctg ccgtccggca tcgtccctgc cgttcgccag ccgctggcgg
     2281 aggataaatc attactgccg tttttcagca atgagcgtgt gattcgtgct gttggtggcg
     2341 ctggtgcact gtctgactgg ctgttgcgtc atgtcaaatc ctgccagtgg cctcatggtg
     2401 actatcatca cagtgaaacc gttattcacc gttatggtac cggcgcaatg gtgttgtgct
     2461 ggcactgcga caaccagctg cgtgaccaga catccgaatc actcgagcaa cttgctcatc
     2521 aaaacctgtc agcatggatg attgacgtca tcggtcacgc aataagcggt acgcaggagc
     2581 gtgaattatc tctggctgaa ttatcctggt gggcggtccg caatcaggtg gcggacgcgc
     2641 taccggaagc ggtattacgt cgttcgctgg ggttgcgtgc ggaaaaaatc cgctcaatgt
     2701 accgtgaaag cgacatcgta ccgggagagc agaccgccac catcatactg aagcagcgca
     2761 caaaaaatct tgcgccgctg cctcacgccc accagca
//
LOCUS       DAZFDC010000274         2777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFDC010000274 DAZFDC010000000
VERSION     DAZFDC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.276"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..546
                     /locus_tag="ACHJ48_004915"
     CDS             <1..546
                     /locus_tag="ACHJ48_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4710266.1"
                     /translation="KTGAEAIRALAMQIPAFRQKLSDGWYLVRIAGRDTGENELSARL
                     NEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIAVAWWNPVGWLGAAAVSGMYAAGA
                     SMILGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            607..>2777
                     /locus_tag="ACHJ48_004916"
     CDS             607..>2777
                     /locus_tag="ACHJ48_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4710267.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALRETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYDPEKRTYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWTDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEGIVDNGAHFDGDQSSTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTP"
BASE COUNT          603 a          714 c          933 g          527 t
ORIGIN      
        1 aaaacggggg ccgaagccat ccgggcgctg gccatgcaga tcccggcgtt tcgtcagaag
       61 ctgagcgacg gctggtatct ggtacggatt gccgggcgtg atacaggtga aaatgaatta
      121 tctgcccgtc ttaatgagcc gctggcaaat ggtgccgtga tccacatcgt gccgcgcctg
      181 gcgggtgcta aaagtggcgg tgtgtttcag gcagtgctgg gtgcggcgct gattgcggtg
      241 gcatggtgga accctgtggg ctggctgggg gccgcggctg tatcgggtat gtatgcagca
      301 ggggccagta tgatcctggg tggtgtggcg cagatgctgg caccgaaagc cagaactccc
      361 cgtacacaga caacggataa cggcaaacaa aacacctatt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gcccgttctg tacggtgaaa tgcgcgtggg atcacgtgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttgtcgt ttatggagca
      601 tgacgaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aaggaccgat tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaaggtaat
      781 accaacatct ccggtgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttctgagacg gtgctgggta cggaagtgaa atacgacacg
      901 ccgatcaccc gcaccatcac gtcggcaaac atcgatcgtc tgcgctttac tttcggtgtg
      961 caggcactgc gggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     1021 gttcagatac agcgtaatgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     1081 accacgtcgc agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgtttaat
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcactggtt
     1261 ggcgtgcagg tggactcgga gcagtttggc agtcagcagg tgagccgtaa ttatcatctt
     1321 cgcgggcgca ttctgcaggt gccgtcaaac tatgatccgg aaaaacgcac ttacagcggc
     1381 atctgggacg gaacgttaaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc acccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag taccggacgg ctttggcggc
     1561 acggagccgc gcatcacctg taatgcgtac ctgaccacgc agcgcaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accggccgtc ggataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc tttagcgccc tgaaagaccg ccataatgcc
     1801 gttgaggtga actggactga cccggacaac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattgcccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagtcggg ggcaggcaca ccgtgccggg ctgtggctga ttaaaacgga actgctggaa
     1981 acgcagaccg tggacttcag cgtgggcgca gaagggcttc gccatgtacc gggcgatgtc
     2041 attgaaatct gtgatgatga ctatgcgggg atcagcatcg gtgggcgtgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggcacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt tcagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgg gttcctgacg gtgttgctga atacagcgtg
     2281 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgacgacg gcacgtatgc catcactgcc gtgcagcatg tgccggagaa agagggcatc
     2401 gtggataacg gggcgcactt tgacggtgac cagagcagca cggtgaatgg tgtcacgccg
     2461 ccagcggtgc agcacctgac cgccgaagtc tccgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacgacgga aaccacatac
     2641 cgcttcacgc agctggcgct ggggaactac aggctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttccggattg ccgcaccggc agcgccgtca
     2761 cagattgagc tgacacc
//
LOCUS       DAZFDC010000275         2760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFDC010000275 DAZFDC010000000
VERSION     DAZFDC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.277"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2760
                     /gene="rhsB"
                     /locus_tag="ACHJ48_004917"
     CDS             <1..>2760
                     /gene="rhsB"
                     /locus_tag="ACHJ48_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO4710268.1"
                     /translation="RQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVTSGHPVNP
                     LLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRDN
                     TLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELR
                     LSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHRE
                     AAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEY
                     GRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVRSFTYDDK
                     YRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVL
                     HTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRIT
                     TPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDITRYRYDNPHS
                     DLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYR
                     AYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGL
                     TRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSE
                     DEGLVTHWHYDEADRLTHRTVKGETAERWQYDERGWLTDISHISEGHRVTVHYGYDEK
                     GRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGM
                     KLGDTPLVDFTRDRLHRKTLRRFGRYELTTAYTPAGQLQSQHLNSLQYDRDYTWNDNG
                     ELIRISSPRQTRSYSYSDSGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTL
                     SMWPD"
BASE COUNT          601 a          853 c          868 g          438 t
ORIGIN      
        1 gcgtcagggc gacatgacgc agtatggcgg tagcattgtt cagggttcag ccggggtgcg
       61 cattggtgcc cccaccggcg tggcctgttc ggtgtgcccc ggcggagtga cgtccggcca
      121 tccggtcaat cccctgctcg gtgcaaaggt ccttcccggt gaaaccgaca tcgccctgcc
      181 cggcccgctg ccgttcatcc tctcccgcac ctacagcagt taccggacaa aaacgcccgc
      241 gccggtgggg agcctcggcc ccggctggaa aatgcctgcg gatatccgct tacagctgcg
      301 cgataacaca ctgatactca gtgataacgg cggcagaagc ctgtattttg agcacctgtt
      361 tcccggtgag gacggttaca gccgcagcga gtcactctgg ctggtgcgcg gcggcgtggc
      421 gaaactggat gaaggtcacc ggctggccgc actctggcag gcgctgccgg aagaactccg
      481 cttaagtccg catcgttatc tggcgacaaa cagtccgcag gggccgtggt ggctgctcgg
      541 ctggtgtgag cgggtgccgg aagcggatga ggtgctgcct gcgccgctgc cgccgtaccg
      601 ggtactgacc gggctggtgg accgcttcgg gcgcacacag acgttccacc gcgaagccgc
      661 cggtgaattc agcggcgaaa tcaccggcgt gacggatggt gccgggcgtc acttccggct
      721 ggtactgacc acgcaggcgc agcgggcaga agaagcccgg cagcaggcca tttccggcgg
      781 gacggaaccg tccgcttttc ctgataccct gccgggttac accgaatatg gccgggacaa
      841 cggcatccgt ctgtctgccg tgtggctgac gcacgacccg gaatacccgg agaatttacc
      901 tgccgcgccg ctggtgcgct atggctggac gccgcgcggc gaactggcgg tggtgtatga
      961 ccgtagtggc aaacaggtgc gcagctttac ttacgatgat aaataccggg gccggatggt
     1021 ggcgcaccgt cacacgggcc ggccggaaat ccgttaccgt tacgacagcg acgggcgggt
     1081 gacagaacag ctaaacccgg caggcttaag ctacacgtat cagtatgaga aagaccgcat
     1141 caccatcacc gacagcctga accgccgtga agtcctgcac acgcagggtg aaggcgggct
     1201 gaagcgggtg gtgaaaaagg aacacgcgga cggcagcgtc acgcagagtc agtttgacgc
     1261 ggtgggcagg ctcagggcac agacggatgc cgcaggcagg acaacagaat acagcccgga
     1321 tgtggtgacg ggcctcatca cgcgcatcac cacgccggat ggcagggcat cggcgtttta
     1381 ctataaccac cacagccagt taacgtcagc caccgggcct gacgggctgg aaatacgccg
     1441 ggaatatgat gaatggggcc gtctgattca ggaaactgcc cctgacggcg atatcacccg
     1501 ctaccgttat gataatccac acagtgactt accctgcgca acggaagatg ccaccggcag
     1561 ccggaaaacc atgacgtgga gccgttacgg tcagttgctg agcttcaccg actgttccgg
     1621 ttatgtaacc cgttatgacc atgaccgctt cgggcagatg acggcggtgc accgcgagga
     1681 agggctgagt cagtaccgcg catacgacag ccgtggacag ttaattgccg tgaaagacac
     1741 gcagggccat gaaacgcggt atgaatacaa cgccgccggt gacctgacca ccgtcattgc
     1801 cccggacggc agcagaaacg ggacacagta cgatgcgtgg ggaaaagcca tctgtaccac
     1861 gcagggcggt ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac
     1921 cagtgaaaac ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga
     1981 aaccggcttt gacggccgca cacagcgtta tcaccacgac ctgaccggca aacttatccg
     2041 cagcgaggat gaggggctgg tcacccactg gcactatgac gaagcagacc gcctcacgca
     2101 ccgcaccgtg aagggtgaaa ccgcagagcg ctggcagtat gacgaacgcg gctggctgac
     2161 agacatcagc catatcagcg aagggcaccg ggtgacggtg cattacgggt atgatgagaa
     2221 aggccggctg accggtgagc gtcagacggt gcatcacccg cagacggaag cactgctctg
     2281 gcagcatgag accagacacg cttacaacgc gcaggggctg gcgaaccgct gtataccgga
     2341 cagcctgccc gccgtggaat ggctgaccta tggcagcggc tggctggcag gcatgaagct
     2401 cggcgacaca ccgctggtgg atttcacccg cgaccgcctg caccggaaaa cgctgcgcag
     2461 attcggccgt tatgaactca ccaccgctta tacccctgcc gggcagttac agagccagca
     2521 cctgaacagc ctgcagtatg accgcgatta cacctggaac gacaacggcg aactcatccg
     2581 catcagcagc ccgcgccaga cccggagtta cagctacagc gactccggca ggctgaccgg
     2641 cgttcacacc accgcagcga atctggatat ccgcatcccg tatgccacgg acccggcagg
     2701 taaccgcctg cccgacccgg agctgcaccc ggacagcacc ctcagcatgt ggccggataa
//
LOCUS       DAZFDC010000276         2667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFDC010000276 DAZFDC010000000
VERSION     DAZFDC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.278"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..231
                     /locus_tag="ACHJ48_004918"
     CDS             13..231
                     /locus_tag="ACHJ48_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO4710269.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            233..790
                     /locus_tag="ACHJ48_004919"
     CDS             233..790
                     /locus_tag="ACHJ48_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO4710270.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1081..1236
                     /locus_tag="ACHJ48_004920"
     CDS             1081..1236
                     /locus_tag="ACHJ48_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO4710271.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1356..1700
                     /locus_tag="ACHJ48_004921"
     CDS             1356..1700
                     /locus_tag="ACHJ48_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO4710272.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1822..2094
                     /locus_tag="ACHJ48_004922"
     CDS             1822..2094
                     /locus_tag="ACHJ48_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710273.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2096..>2667
                     /locus_tag="ACHJ48_004923"
     CDS             2096..>2667
                     /locus_tag="ACHJ48_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO4710274.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLP"
BASE COUNT          692 a          605 c          725 g          645 t
ORIGIN      
        1 gggcggtaag aaatgaaaca ttatttagaa aaaaattacc cacgaaagag cagaacaaca
       61 gagtttctgt ttttcattct gtttatagtg ttgatgatac cgatatcccc gctattactg
      121 gtctggataa ttggaaggac atttgaacca gttattgagc tatataccga tgtgacatgg
      181 gaatcattca gcgcactgca caataaaatt aatccgtata aggaaaactg atatgagcac
      241 tattaccaga gaacgcgcgg agattaaatc atacatcaca ggcttcctga gcgactcggc
      301 gcacgataac aagtcttcag acagcctgct ggctaatgtg tttcgtatcg cgctggcatc
      361 actggaagca gagccgatag caatggtagt gcctgatgaa atggatttgc ttacctgcca
      421 tctcgacggt gtaactaaaa catatgctga tggctggaac gcctgccgcg tcgccatgct
      481 tcaggccgga aactttcggg aaaataagaa ttcgtcaacc aacaattttc gggaaatctc
      541 ggaaacgtca accagatctc cgataactct ggatggctgg ataagctgta ctgagcgaat
      601 gcctgaaaag agccagaacg tgcttatttc gatgaatatc gatagcgagg ctgggccatt
      661 aatatattcc gcacgctatc tcggaggcac gttccggcgc ggaggtatag cagttagtcc
      721 gggtaatgat cttaggcaag caacccactg gatgtcgcta ccagaaccgc cgcaggaggt
      781 gaatcaatga cctggcctga agcattcaca acggtaggaa ttgcgatggc ggtggcgctg
      841 gtggtgtatt cgatttgccg ctggggataa aaacggtttg cgggaaaagg atagttaagt
      901 agaattgctg cgggtgcttg aggctatctg cctcgggcat gaacaccaac ggcagataga
      961 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggctcaa
     1021 tccatgctga acacatgtag gttagcctct tacgtgccga aaggcaagga gaagcaggct
     1081 atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg
     1141 acggcactgg taacgaggaa agacctctgc gaggtgcgaa tccgaaccgg tcagacggag
     1201 gtcgctgtct tcgtagacta cgaatctgag aagtaagaga ccaggcgggg gagtaatctc
     1261 ccgccacctc tgatgtgtca ggcatcctca acgcacccgc gctttaccat actgaaaatg
     1321 ctgtttgaat gttcatctct gaaagaggac tatgaatgaa aaaggtattg attgcagcac
     1381 ttatttccgg tgtgtctttt ggcgcttttg cacagcaggg tggtttccag gggccagaag
     1441 cagagcgttc aacagtagcg caggcaaaag aactgaagga tgatgcatgg gttatccttg
     1501 aagggagcat cgttaaaaaa gtgggtgatg aacgttatga gtttcgtgac aatagcggga
     1561 caattgtcac ggatattgat gacagcgtat gggccgggca gaatgtttct ccgaaagaca
     1621 aagtaagaat tgagggtgaa attgataaag acctgagcag tgttgaagtt gatgtaaagg
     1681 cactgaaatt attaaagtaa ccgcccctgc ttgttaagcc cgtcttactg acgggttttc
     1741 tgtttgtaca ttccggcgta ttgccttaca attcgcgcag tcagcctgaa caactgacac
     1801 ctgctgtcac cggagaatcc gatgacacaa cacataaaat cccacaattc tgaagccgac
     1861 ccggaaatta agcaggggag gcgttttcgt gcgcctcagt atggctggtt tcactatctg
     1921 ttctgtacga tcgatgaggc agatatgctt caagaggcgt atctgcgtcg cggtgtccgt
     1981 gtggagcgga gtctgaacgc tgatcgtctg acctggaccg tttctgtata tcttcctgtt
     2041 cgtgcacatc tgccacggac acatgcctgc taccgtcagc gcgtctggag gtaatgtgcg
     2101 ggtattactt cgacctgttc tggttccgga actcgggctg gtggtcctta agcccggtcg
     2161 tgaatcattg ccagtttttc atcgcggcag ggtgctggtg gagccggaac cgaaaaacat
     2221 gcgggcgctg ccatctggag cggttcctgc tgttcgccag ccgctggcgg aagataaatc
     2281 actgctgcca tttttcagcg atgagcgggt gattcgtgca gctggcggcg ctggtgcact
     2341 gtctgactgg ttattacgtc acgtgaaatc ctgccagtgg ccacacggcg attatcatca
     2401 cagcgaaacc gttattcaca gttacggtgc tggcgcaatg gtgttgtgct ggcactgcga
     2461 caaccagctg cgcgaccaga cctccgaatc acttgagcaa cttactcaac aaaatctgac
     2521 agcctggatg attgacgtca tacgccatgt aatgaatggc acgcaggagc gggaattatc
     2581 gctggctgaa ttatcctggt gggcagtctg caatcaggtg gtggacgcat tacctgaggc
     2641 agtatcgcgt cgctctctgg gattacc
//
LOCUS       DAZFDC010000277         2607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFDC010000277 DAZFDC010000000
VERSION     DAZFDC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.279"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            146..268
                     /locus_tag="ACHJ48_004924"
     CDS             146..268
                     /locus_tag="ACHJ48_004924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710275.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            265..831
                     /gene="mntP"
                     /locus_tag="ACHJ48_004925"
     CDS             265..831
                     /gene="mntP"
                     /locus_tag="ACHJ48_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO4710276.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(828..1637)
                     /gene="rlmA"
                     /locus_tag="ACHJ48_004926"
     CDS             complement(828..1637)
                     /gene="rlmA"
                     /locus_tag="ACHJ48_004926"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO4710277.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQKSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQILAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1803..2012)
                     /gene="cspE"
                     /locus_tag="ACHJ48_004927"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1803..2012)
                     /gene="cspE"
                     /locus_tag="ACHJ48_004927"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO4710278.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2025..2168)
                     /locus_tag="ACHJ48_004928"
     CDS             complement(2025..2168)
                     /locus_tag="ACHJ48_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO4710279.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT          684 a          620 c          612 g          691 t
ORIGIN      
        1 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccaattt
       61 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      121 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      181 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      241 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      301 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg ccaccctcca taaaccgaaa
      361 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      421 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      481 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      541 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      601 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      661 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      721 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      781 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
      841 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtatt
      901 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
      961 tcatcaccgc gaagacgcat cggataacac aactcatcac tcttctgtaa tgtaaaacct
     1021 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1081 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1141 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1201 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1261 gccgccgctt ttatcgctac cttcgaaaca tccagaccaa acgtggtgat ttcgggcaac
     1321 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1381 gccttttcat taagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1441 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1501 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1561 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg taagaggctg atggcaaagt
     1621 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1681 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1741 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1801 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     1861 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     1921 acgaacacgt ctttgctgcc atcagcagga gtaatgaagc caaaaccttt agactcgttg
     1981 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2041 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2101 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2161 ccacacataa acagaactgt acctcgttta acccgaaatc tgttatcaca tacaacgtta
     2221 attatggcaa gccattttta aacatgtctc gatcagacgc acaaatctcg atacgtcttc
     2281 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2341 gtcagttccg cagatactta aactgacgta gtttctaaca tagttcaata agttcagttg
     2401 ttccaggtca gtgggagaag ttattacata gtgcgtgcaa tatcacattt tctgctatgc
     2461 aatgaataaa aagttatatc actttttctc ataaaacagg cagttaacgg ctctcaatca
     2521 ccccaaagag aagtcaatcc caaaggaatt ggaaatttaa aataagaaaa attgatgaat
     2581 aagcaaaaaa aatcaagaga gaaacgt
//
LOCUS       DAZFDC010000278         2586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFDC010000278 DAZFDC010000000
VERSION     DAZFDC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.280"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..2201
                     /locus_tag="ACHJ48_004929"
     CDS             54..2201
                     /locus_tag="ACHJ48_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO4710280.1"
                     /translation="MLYNIPCRIYILSTLSLCISGIVSTATATSSETKISNEETLVVT
                     TNRSASNLWESPATIQVIDQQTLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRG
                     EASSRVLILIDGQEVTYQRAGDNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIV
                     NFITKKGGDKLASGVVKAVYNSATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPD
                     GRLPNTNYRNNSQGVWLGYNSGNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKL
                     EREKVGVFYDTDVDGDYLKKIHFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKT
                     DTHDKQYTQAVTLQSHFSLPANNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETR
                     TRSYYESEQSTVSLFAQNDWQFADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTS
                     LARESASDHEMVTSTSLRYSGFDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNP
                     DLKAEHSNNFELGARYNGNQWLIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSY
                     YYYDNIDRAKTWGLEISAEYNGWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIG
                     LKHTLVMGQANIISDVFIRAASSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRIN
                     LALNNLTDKRYRTAHETIPAAGFNAAIGFVWNF"
BASE COUNT          763 a          566 c          574 g          683 t
ORIGIN      
        1 aaaataatta tcattacagc aatcatttgt actttgtatt aatgagggat gaaatgttat
       61 ataatatacc ttgtcgaatt tatatccttt ccactctgtc attatgcatt tctgggatag
      121 tttctactgc aaccgcaact tcttcagaaa caaaaatcag caacgaagag acgctcgtcg
      181 tgaccacgaa tcgttcggca agcaaccttt gggaaagccc ggcgactata caggttattg
      241 accaacaaac attgcagaac tccaccaatg cctccatagc cgataatttg caggacatcc
      301 ccggagtaga gataacagac aactccttgg caggccgtaa acaaatccgc attcgtggcg
      361 aagcatcctc ccgtgtttta attctcattg atggtcagga ggtaacttat cagcgcgccg
      421 gagataatta tggtgtggga ctgttgatag atgagtctgc gctggagcgt gttgaggtag
      481 tgaaaggtcc atattccgta ctgtacggtt cacaggcaat tggcggtatt gttaacttca
      541 tcaccaaaaa gggaggtgac aaacttgcat ctggagttgt gaaagctgtt tataattccg
      601 caacagcagg ctgggaagaa tcaatcgcgg tccaggggag catcggtgga tttgattatc
      661 gcatcaacgg tagttattct gatcagggca atcgtgatac gccggatgga cgtctgccga
      721 ataccaacta tcgtaacaat agtcagggtg tatggttggg ttataactcc ggaaaccatc
      781 gttttggcct ctcgcttgat cgctacagac tcgcgacaca aacttactat gaggatccag
      841 acggaagtta tgaggcattt agtgtcaaaa tacctaaact tgaacgagag aaagttgggg
      901 tattctatga cacagacgtg gacggtgact atctaaaaaa aattcatttc gacgcgtatg
      961 agcagaccat ccagcgccaa tttgccaacg aagtaaaaac gacacagcct gttcccagtc
     1021 cgatgattca ggctctgacc gttcataaca agactgacac ccatgataag caatacactc
     1081 aggcggtcac attgcagagt cacttttcgc tgcctgctaa taatgaactt gttaccggtg
     1141 cacagtacaa acaagatagg gtcagccaaa ggtccggtgg catgacctca agcaaatctc
     1201 tgaccggctt cattaataag gaaacacgaa ctcgctccta ttatgagtca gagcaaagta
     1261 cagtctcact attcgcacaa aatgactggc aattcgccga tcactggaca tggacaatgg
     1321 gagttcgcca atactggctt tcttcaaagt tgacgcgtgg tgacggagta tcatataccg
     1381 caggcattat aagcgatacc tctcttgcca gagagtctgc gagtgatcac gaaatggtaa
     1441 catctacaag cctgcgctat tcaggtttcg ataacctgga gttacgcgct gcgttcgcgc
     1501 aaggctacgt atttcccaca ctctcccagc tttttatgca gacatctgcg ggcggcagtg
     1561 tcacatacgg aaatcctgat cttaaggctg aacactccaa taactttgaa ttaggtgcac
     1621 gatataatgg taatcagtgg ctgattgaca gcgcagttta ctactcagaa gctaaagatt
     1681 atattgcaag tctgatctgt gatggcagta tagtttgcaa tggtaacacc aactcctccc
     1741 gtagtagcta ctattattat gacaatattg atcgggcaaa aacatgggga ctggaaataa
     1801 gcgcggaata taatggctgg gttttctcgc catatatcag tggcaattta attcgtcggc
     1861 aatatgaaac ttcaacatta aaaacaacta atacaggtga accagcgata aacggacgta
     1921 tagggctgaa acatactctt gtgatgggtc aggccaacat aatctctgat gtttttattc
     1981 gtgctgcctc tagtgcaaaa gatgacagta acggtaccga aacaaatgtt ccgggctggg
     2041 ccactctcaa ctttgcagta aatacagaat tcggtaacga ggatcagtac cggattaacc
     2101 tggcactcaa taacctgaca gacaaacgct accgtacagc acatgaaact attcctgcag
     2161 caggttttaa tgcagctata ggttttgtat ggaatttctg atgacaaaaa caatcgttat
     2221 ggaattcttt gtcctgatct gcccgctttg cgggcaggct aacgaacgta ttgtgtttgt
     2281 tgaaggtttt tcgacgttgt tttccatctg tgggtgaata tttattcttc ccgccagcat
     2341 gactctatac tcattgctac gacaatgact tataaagctg gttactgaac ctataccttt
     2401 gtaattcaaa agtgatatca gttcacaacg caatactatt tttagattat ttactctcac
     2461 ccagatactg aatcaatgga taataccttt tgatttatcc cgacgcggaa gaaggtggtg
     2521 aaccgccccg gaaatcctgg agactaaact ccctgagaaa gaggtaaaca ggatgactaa
     2581 aaatac
//
LOCUS       DAZFDC010000279         2546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFDC010000279 DAZFDC010000000
VERSION     DAZFDC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.281"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHJ48_004930"
     CDS             <1..198
                     /locus_tag="ACHJ48_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4710281.1"
                     /translation="MLANTCMWDYRGDECGYHGPAVADEFDNPTTDIRKDRCSKCMRG
                     CEMRGMVANFGGFLSINKLSQ"
     gene            complement(215..469)
                     /locus_tag="ACHJ48_004931"
     CDS             complement(215..469)
                     /locus_tag="ACHJ48_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4710282.1"
                     /translation="MTKVRDIAPYSVRMPDSLKRDLTIRASKNGRSLNSEIVMILQAA
                     IDEEKSPRSIEGFAQQESEKFREALLKTLSSMYGEDKKPT"
     gene            579..689
                     /locus_tag="ACHJ48_004932"
     CDS             579..689
                     /locus_tag="ACHJ48_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO4710283.1"
                     /translation="MEKEVSRILVRIPQSLKDAITGKAKEECRSFNSEVM"
     gene            992..1870
                     /locus_tag="ACHJ48_004933"
     CDS             992..1870
                     /locus_tag="ACHJ48_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HFO4710284.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALALT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRNDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSPHHY"
     gene            1924..>2546
                     /locus_tag="ACHJ48_004934"
     CDS             1924..>2546
                     /locus_tag="ACHJ48_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4710285.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPEDY
                     FRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGF
                     YRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRE"
BASE COUNT          690 a          541 c          668 g          647 t
ORIGIN      
        1 atgctggcga acacctgtat gtgggattac cggggagatg aatgcggtta tcacggtcct
       61 gcggtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag atgcagcaag
      121 tgcatgcgcg ggtgtgagat gcgcggcatg gtggctaatt ttggcggttt cctttccatt
      181 aataaacttt cgcagtaatg gattatgccc accatcaggt gggtttttta tcttcgccgt
      241 acatgctgct gagagtctta agaagagcct ccctaaattt ttcagattct tgttgagcaa
      301 aaccttctat tgatcttggt gatttttctt catcaatagc agcttgcaag atcataacta
      361 tttctgaatt taatgagcgt ccgtttttcg atgctcttat ggtcaggtcg cgcttgaggc
      421 tatctggcat tcttacgcta tatggggcta tgtctctaac cttcgtcata cttacaccgt
      481 gataatcaca ttgatatcac agtgtattca aaaaaacttt gacttgatat ggtcacagtg
      541 atacgtttgt gattcataat aactcacgcg gtggcgatat ggaaaaagaa gttagcagaa
      601 ttttggtgag aatacctcag tcgctaaaag atgcgattac aggaaaggcc aaagaagaat
      661 gccggtcgtt taactcggaa gtgatgtgat tgctggacag cctgaagaga gaggggataa
      721 cggtatgagt aaagaatgtt gttttttgcg gcattagcga atcagacgct gatcaaacat
      781 acatttactc taaagaaaca ggtcggatgc tgtgtagtga ctgcgtgttt gacatcataa
      841 gatacaagta tcttggatgt tctgccagca ttagcaatat aggtgaagtg tacgaaggga
      901 aagatataac tgatagagca gaaagttgaa gccccaactg ctgtaacagt cagggcttcg
      961 ttatcaacaa atcggcttag gaaatattga catgaaaagt atagcaaagg cacaaaacga
     1021 tttcactatc ttcaaattcg gcgacagtga aatccgcgtc atcaacaagt gcggtgagcc
     1081 gtggtttgta gctaaagatg tttgtgatgc tttagctttg actaactcac gcaaggcgct
     1141 tactgcactt gatgacgatg aaaagggagt aactttaagt tacacccttg gtggtgagca
     1201 gaatctaagc attgtgagcg aatcaggtat gtatacattg gttctgcgct gccgcgatgc
     1261 agtcaataaa ggttcagtcc cgcacaaatt ccgcaagtgg gtaacagcag aagttctgcc
     1321 ttcaattcgc aaacatggcg agtatgtaaa aggaaagaaa accactgttg aggaaagaac
     1381 accgctacgc gatgcagtaa acatgctggt aggaaagaaa ggacttcgca atgacgatgc
     1441 atacaatatg gttcatcagc gttttggtat tgacagcatt gatgagcttt caattgaaca
     1501 aatcccgctt gccgtagagt acatccacag ggtagtgctt gaaggtgaat tcattggcaa
     1561 acaagagaag agcaccaacg agctttccgc aaaagaagca aacagccttg tatggctatg
     1621 ggattatgcc aaccgctcac aggcattatt ccgcgaactg tatccggcgc taaaacaaat
     1681 tcaatcgaac tattccggca gatgctacga ctacggtcat gagttctcgt atgttattgg
     1741 aatggcgaga gacgttttaa ttaatcacac acgagatgtt gatatcaatg agccagacgg
     1801 accaacgaat ctttccgcat ggatgagact taagaataaa gaattacctc cttcaccgca
     1861 tcactactaa cagattgcca acgaaatgac ccagcttcgg ctgggttttt atcaggagtt
     1921 ctcatgctct atagcaatat attggcgcac gcccggcgat gtgcaccagc ggagtcgtgc
     1981 ggcttcgtgg tgagaacgcc ggaaggggaa agatattttc cctgcgtgaa tatctccggt
     2041 gagccggaag attatttccg gatgtcgccg gaggactggc tgcgggcaga aatgcagggg
     2101 gagattgtgg cactggtcca cagccacccc ggtggtctgc cctggctgag cgaggccgac
     2161 cggcggctgc agataaaaag tgcactgtcc tggtggctgg tctgccgggg ggaaattcat
     2221 aaattccgct gtgtgccaca tctgacagga cggcgctttg agcacggggt gacggactgt
     2281 tacacgctgt tccgggatgc ctaccatctg gcgggaattg atatgccgga ttttgagcgt
     2341 gaggatgact ggtggcgcaa cggtcagaac ctttacctgg acaatatggc ggtcaccggc
     2401 ttttaccggg tgcccctgtc ctctgcacag gcgggcgata tcctgctgtg ctgctttggc
     2461 gcatcggtgg ccaatcatgc cgccatatac tgcggcaacg gtgaactgct tcaccatctg
     2521 cctgaacaac tgagtaaacg ggagag
//
LOCUS       DAZFDC010000280         2534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFDC010000280 DAZFDC010000000
VERSION     DAZFDC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.282"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            317..1051
                     /locus_tag="ACHJ48_004935"
     CDS             317..1051
                     /locus_tag="ACHJ48_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFO4710286.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALTQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            1048..1548
                     /locus_tag="ACHJ48_004936"
     CDS             1048..1548
                     /locus_tag="ACHJ48_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO4710287.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            1622..2194
                     /locus_tag="ACHJ48_004937"
     CDS             1622..2194
                     /locus_tag="ACHJ48_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO4710288.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTVLAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
BASE COUNT          683 a          625 c          673 g          553 t
ORIGIN      
        1 ataaaaataa acagtaataa acagcataaa aagcccatca acgggtgaac agtggtgaac
       61 agacggtgaa cagtcattac tgcgattgtt catcctttaa cttactgtat tacttatctt
      121 ttttcttatg gtgaacagag gtgaacagta aaatataaaa aaacaaacag taagccggtt
      181 tttcctgcga ccttttcctg gcttgccggt ctgaggatga gtctcctgtg tcagggctgg
      241 cacatctgca atgcgtcgtg ttgctgtccg gtgtacgtca caattttctc aacctgaagt
      301 gacgaggagc cggaaaatgt ctgacaacac catccctgaa tatctgcaac ccgcactggc
      361 acaactggaa aaggccagag ccgctcatct tgagaacgcc cgcctgatgg atgagaccgt
      421 cacggccatt gaacgggcag agcaggaaaa aaatgcgctg acgcaggccg acggaaacga
      481 cgctgacgac tggcgcacgg cctttcgtgc agccggtggt gtcctgagcg acgagctgaa
      541 acagcgccac attgagcgcg tggcacgccg ggagctggta caggaatatg acaatctggc
      601 cgtggtgctg aatttcgaac gtgaacgcct gaaaggggcg tgtgacagca cggccaccgc
      661 ctaccggaag gcacatcatc accttctgag tctgtatgca gagcatgagc tggaacacgc
      721 cctgaatgaa acctgtgagg cgcttgtccg ggcaatgcat ctgagcattc tggtacagga
      781 aaatccgctc gccaacacca ccggccatca gggctacgtc gcaccggaaa aggctgtcat
      841 gcagcaggtg aaatcatcgc tggaacagaa aattaaacag atgcaaatca gcctcaccgg
      901 cgagccggtt ctccggctga ccggactgtc agcggcaaca ctcccgcaca tggattatga
      961 ggtggcaggc acaccggcac agcgcaaggt gtggcaggac aaaatagacc agcagggagc
     1021 agagctgaag gccagaggac tgctgtcatg atttactgcc cgtcgtgtgg acatgttgct
     1081 cacacccgtc gcgcacattt catggacgat ggcaccaaga taatgattgc acagtgccgg
     1141 aatatttatt gctctgcgac atttgaagcg agtgaaagct ttttctctga ctgtaaagat
     1201 tcaggaatgg aatacatttc aggcaaacag agataccgcg attcactgac gtcagcctcc
     1261 ggcagtatga aacgcccgaa aagaatgctt gttaccggat attgttgtcg gagatgtaaa
     1321 ggccttgcac tgtcaagaac atcgcggcgt ctgtctcagg aagtcaccga gcgtttttat
     1381 gtgtgcacgg atccgggatg tggtctggtg tttaaaacgc ttcagaccat caaccgtttt
     1441 attgtccgcc cggtcacgcc ggacgaactg gcagaaagcc tgcatgaaaa acaggaactg
     1501 ccgccagtac gcttaaaaac acaatcatat tcgctgcgtc tggaatgagg gctgccggtt
     1561 aacaccggcc gtcgccgcac accgtatttt tattcttcag catgatgaga aagagataac
     1621 gatggaaagc acagccttac agcaggcctt tgacacctgt cagaataaca aagcagcatg
     1681 gctgcaacgc aaaaatgagc tggcagcggc cgaacaggaa tatctgcggc ttctgtcagg
     1741 agaaggcaga aacgtcagcc gcctggacga attacgcaat attatcgaag tcagaaaatg
     1801 gcaggtgaat caggccgccg gtcgttatat tcgttcgcat gaagccgttc agcacatcag
     1861 catccgcgac cggctgaatg attttatgca gcagcacggc acagtactgg cggccgcact
     1921 ggcaccggag ctgatgggct acagtgagct gacggccatt gcccgaaact gtgccataca
     1981 gcgtgccaca gatgccctgc gtgaagccct tctgtcctgg cttgcgaagg gggaaaaaat
     2041 taattattcc gcacaggata gcgacatttt aacgaccatc ggattcaggc ctgacgcggc
     2101 ttcggtggat gacagccgtg aaaaattcac ccctgcgcag aacatgattt tttcgcgtaa
     2161 aagtgcgcaa ctggcatcac gtcagtctgt gtaaaatttc ccgaaaatcc gcctgttttt
     2221 actgaaaaaa gccatgcatc gataaggtgc atggctttgc atgcgttttc ctgcctcatt
     2281 ttctgcagac cgcgccacgc ccggcgtggc ctgagcgtgt cagtgcaact gcattaaaac
     2341 cgccccgcaa agcgggcggg cgaggcgggg aaagcactgc gcgcaagtgg tgttttataa
     2401 tcaaagacag ataactgtat tattatattg gtaattaata gttgtaatgt aatgtttgtt
     2461 gggtaaagca ttttgtgtaa aaagccgcta ctatagcggc ttgaatattg gaactatata
     2521 tttagcaagc gatc
//
LOCUS       DAZFDC010000281         2488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFDC010000281 DAZFDC010000000
VERSION     DAZFDC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.283"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            551..742
                     /locus_tag="ACHJ48_004938"
     CDS             551..742
                     /locus_tag="ACHJ48_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO4710289.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(795..1028)
                     /locus_tag="ACHJ48_004939"
     CDS             complement(795..1028)
                     /locus_tag="ACHJ48_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710290.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(1124..1747)
                     /locus_tag="ACHJ48_004940"
     CDS             complement(1124..1747)
                     /locus_tag="ACHJ48_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4710291.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFRFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1836..2345)
                     /locus_tag="ACHJ48_004941"
     CDS             complement(1836..2345)
                     /locus_tag="ACHJ48_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710292.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          719 a          640 c          526 g          603 t
ORIGIN      
        1 cagcctcctg acgatacctc ccaccatgtt ttgcgatggc acgctaccgc ccttccggta
       61 tttacccgca tcaaccggta ccagaatgct gagtaacaaa ccagctagaa ttgacttaat
      121 tttgatcatt atagaataat gatctgaact acagcaacat gacagattta tcgatcgata
      181 taacacaaat gaacagttct ttattctgac aaatatcgca aaaacagcac attacaaaat
      241 ttataaccat aattactgga aaagtgcaga aaattattcc actgaatgct tttaatacag
      301 ttggttagct taaaaaatag caatttttga tttcagtcat tgaaagttgc aatgtttgac
      361 gattcaacag catttaagtg agtttcagga tatgggaagg acatgaaagg aggagcgcaa
      421 taaaatacac aggaagaagg ggaaaatgtt tcgtaaaaac agaaaattaa cagcgcacat
      481 caggaaacgc agtctcctga ttcagtgcta ccacatgaaa atttagttaa ctgttaccgt
      541 actaaaattt atgtcagaag atgttccatt accgaaagtc aatcagcgat acaaagacga
      601 tcatggtgcg cttgtcaccc tgaccagtgt tgaggaaacc cgggtggtgt ttatgcagga
      661 cggctatccg catccctgca tgcgaccgat gtacaacttt ctgggaaaat tcaaacccga
      721 accccgggaa gaaacagaat aaaacgaaat ccctgccggc aatcacacta cctgatagca
      781 gcataatcca cttattaatt ctcatccccg gagacaagcc cagaaaacca ctccgggtgt
      841 tcactgtatg ccgtgttcag cgcatccctg gaaacgttta tataaaagct gtcctgcaat
      901 ttgcgggtaa caacttctgc caggcgattc cggttatcca ccagtccggc ataacctctc
      961 tctgccagcc actcattcag ttcatgttcc agagacagat tgataatatc ataatcggac
     1021 cttgccattc ccgtttcctt agcacatgtt ttcaggcacg gggataaagt ataaagaatg
     1081 tcatactgct gctgccagag caatattccc cctgatggtt gtatcagaga tggtatgccg
     1141 gtactttgcg ggttgttccc gtcaggttat cgtgcacctc cacggtcgtg gtcaccaccg
     1201 gcattccggc ctttctcagc ctcagaacat cagccgcaat acgctgactg ccaaacctga
     1261 acaggccgtc cagtgcagtc accagtaaac ccgcctccag tgcatacttc agccgttcac
     1321 ggggcgcttt cacttcccgg gcaacctgct gatatggcaa tgatgtgttt tcattcccaa
     1381 ccacgcgacg aatacggtga gacagatgat accggtatgt atccggcaca ccagaaaggc
     1441 tggccttcag gctgtacacg cagccaaacc gtttatcgtt aaacaccaca tttttctggc
     1501 tgatgcccca ttcttcacgc agcgcggcaa tcaattgcgg tgtacgggta agcagcaagc
     1561 gaaaaggcag ttcaaaactg gtgacgtaat ccacattcag cagtgcaata cgcaaccgtt
     1621 cttctggccc ggcctctgtc tgccggcact cctccagaac atcctgccac tgccggcgaa
     1681 gacgggggga ttcgttccgt tctgtaaaac agtatttatc cgccagatag tcaattcgtg
     1741 tacgcatact gaaaggaatt ccgtataaag attcagcaga caaaacttta tcagccagta
     1801 aaaattaacg gaagcattga tattcctccc gacaatcacc ggatgattgt tgcaataacc
     1861 tgcgacataa aggactgaac agccgttttt aacgccacgt attgccctga tgaaccaggc
     1921 cgggccaccc gaaaacatct ttccacccgg cattgtccgc cagcacttat caccgaccag
     1981 gtctgttgca gtaaatccac cagttgcgca cattcttcat cagccatgac gccgactcgc
     2041 ggatggcaaa tactcgtctg gctgagcagc aacagacatc gtatctgtgc tccttcatgt
     2101 tgttcccgca accagcgtaa taccaccggc gctgatgggc tgaccgtgtg attatgctcc
     2161 gtaatacgtt cgtgtacccg tcggtgaaag gaactacaaa atgtctgaat ctgttgacca
     2221 tagattcctt ctgccgaata gagcgtgaag tgaaatgcca acagatttgg ccagtggtcc
     2281 accgcctgct gaacaaaacg ccggatttcc cccggctctg aaattaaggc ttcggttatt
     2341 tgcacgaaat gttccttttc aacccctgtt gaattttgtc aagccggtgc agacacagta
     2401 aagcgcgcat caacacctga aaggtacgat acaaacacaa gttacaaaca ataagaacgg
     2461 aaggacaatt aatcagtgag gaataata
//
LOCUS       DAZFDC010000282         2479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFDC010000282 DAZFDC010000000
VERSION     DAZFDC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.284"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..132
                     /locus_tag="ACHJ48_004942"
     CDS             <1..132
                     /locus_tag="ACHJ48_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4710293.1"
                     /translation="SRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            83..784
                     /gene="traT"
                     /locus_tag="ACHJ48_004943"
     CDS             83..784
                     /gene="traT"
                     /locus_tag="ACHJ48_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO4710294.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(832..1083)
                     /locus_tag="ACHJ48_004944"
     CDS             complement(832..1083)
                     /locus_tag="ACHJ48_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4710295.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1107..1397)
                     /locus_tag="ACHJ48_004945"
     CDS             complement(1107..1397)
                     /locus_tag="ACHJ48_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO4710296.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            2083..2442
                     /locus_tag="ACHJ48_004946"
     CDS             2083..2442
                     /locus_tag="ACHJ48_004946"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO4710297.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
BASE COUNT          747 a          542 c          520 g          670 t
ORIGIN      
        1 agccgggatg aggccatctc agtaatacgg gaatacattg agattttcta caatcgtcag
       61 cgtcgtcact ctcgtctggg gaatatctcc ccggcagcct tcagggaaaa atatcatcag
      121 atggctgctt aaaaaaagaa caaatggtag tgtccgctat tgtcagtaca cctcaaccat
      181 ctggcttgaa cctgcgagcg aacgcaccgt tttcctgcag atcaaaaaca cgtcggataa
      241 agacatgagt ggactacagg gaaaaattgc cgatgcagtc aaagccagag gttatcagat
      301 catgacttcc ccggataaag cttactactg gatccaggct aacgttctca aagcagacaa
      361 aatggatctg cgtgaatctc agggatggct gagtcgcggt tatgaaggtg cagcagtcgg
      421 agctgctctt ggaggtggaa tcacagcgta caataccggg tcggcaggta ccactctcgg
      481 tgttggactg gctaccggtc tgattggcat ggccgcggat gcaatggtgg aagatataaa
      541 ttacacaatg attacagatg ttcagattgc cgagcgaacc agaaccagcg tcagaacgga
      601 taatgttgca gctttgcgtc agggaacatc aggctccaaa attcagacaa gcacagaaac
      661 cggcaaccag cataaatacc agacccgggt tgtatccagt gccaaccagg ttaatctgaa
      721 gtttgaagaa gctaaacctc atctggaaga ccagctggca aaatcaatcg ccaatatact
      781 ctgatataaa tatcccggtg gccattcacc tggcctccgg ccctcttagt ctcaacgttc
      841 agacaattca gatattattt tccctgtcac accatatacc catgccggaa cagaatctaa
      901 tgacggtacc agtaaattct tcagcgttcg gtttgcctga cgcccgtagc tgtatttgtg
      961 tctgtttctt cttatacaag agcagtagta caatttctct tccagagtca gttcaacttt
     1021 aggacgtaac caataaagat ctcttccact ataaaatggt gaatcctgta caaacgattc
     1081 cataacagaa cctcctgctg ctacagttaa cacagctgct ggcatggact aataccatct
     1141 tttaatttaa ccctggcgga gacgccatta tttttggacg ttcttgaaac aaaattaatt
     1201 cctgaagaat ctttctccag tttattcagc tcaagattca gtccataatt aacgtcaaat
     1261 aactctgata aaggtatcag tttatgttca ggataatgat tatcccgacg acaacaaata
     1321 ttaaaatcct gcatattacc cgacatcagg agttcaaaac cgtccccggc atcattcata
     1381 ctggcagata aattcattac ccagtttagt gctaatcaac aggtacggct taacttacag
     1441 gaattccccg ggaggggcgg actcatcctc agttagcggc aaaatcgcat cacagccaat
     1501 cgcctgtctc gattccccat cggcgaacaa tgtatgtggc cacaaaatcc tggctggctc
     1561 agaacgaaga gcatctgagc aaagaggtca tcaatctggt atgaatatct ggcagaaaat
     1621 atcccctgaa tccacaactg ctgtgcatat ctgctcaaat cagcatcaat cataaaccag
     1681 taatcacagt gcctgtgatt gggttacagg aataagtccc ccttcataag gcatatcatc
     1741 aataccggtg aaggaaaaga aagcatcaga tggcctgata gtctgcgtca gtcaatgaca
     1801 tcagtgcttt atccggaaca atgtatccat gttggcgaca gagaaagcca ttgccatgaa
     1861 cagttttgtt tatccccgga aagtcatttc tgttacaaat ttatattgac aaacttgctt
     1921 tcgatgagaa acatactatt tcaatgaaat gcagaatgag gcaaaatttc gttatgtttt
     1981 ttaattcccg aaagaataaa tgtggcttat tacaaaatcc tgttaatgtt aactgatatt
     2041 ttataatgca tattcagaca tataattaaa taagaggtat ttatggctaa tacgaccact
     2101 ggtgttacca gaattattaa agcagcgggt tattcatgga aaggcttatg tgcggcatgg
     2161 cgctatgaag cagcctttcg tcaggagtgc attctggttc tgtcagcact gataatatcc
     2221 ttttggctgg atgttgactc tgttactcga gtattactca ttggttcggc aatgatggta
     2281 attattgttg aaataattaa cagtgcaatt gaagcagttg ttgacagaat tggttcagaa
     2341 catcacgaat tatcaggcag agcaaaagat atgggttcag ccgcagtgtt gattacgatt
     2401 ttagttgcta tcattatatg gggagtttta ttactttcat aacgcccaac atctaaatta
     2461 ttcacagtta cctggttaa
//
LOCUS       DAZFDC010000283         2467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFDC010000283 DAZFDC010000000
VERSION     DAZFDC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.285"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            324..530
                     /locus_tag="ACHJ48_004947"
     CDS             324..530
                     /locus_tag="ACHJ48_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4710298.1"
                     /translation="MEKITTGVSYTTSAVGTEYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(786..1058)
                     /locus_tag="ACHJ48_004948"
     CDS             complement(786..1058)
                     /locus_tag="ACHJ48_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710299.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1218..1751
                     /locus_tag="ACHJ48_004949"
     CDS             1218..1751
                     /locus_tag="ACHJ48_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4710300.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1972..2085
                     /locus_tag="ACHJ48_004950"
     CDS             1972..2085
                     /locus_tag="ACHJ48_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710301.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            2087..2407
                     /locus_tag="ACHJ48_004951"
                     /pseudo
     CDS             2087..2407
                     /locus_tag="ACHJ48_004951"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2397..>2467
                     /locus_tag="ACHJ48_004952"
     CDS             2397..>2467
                     /locus_tag="ACHJ48_004952"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFO4710302.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNG"
BASE COUNT          634 a          501 c          678 g          654 t
ORIGIN      
        1 accagttaca gatgctttat taaggaaaaa aacagccagc actgactttc ggtggagagg
       61 tgctggctca gaaggatagt tggatttcac atgatactta tgcctggcgg tatattttct
      121 gacagacagt gacgggtgtt gtcaagatat tgtgtcattt ataacctgaa tcaggggagg
      181 ccggaatgtt atctggcatt tttagcagag cctgaatgcc ataatcacgg ctcccggagt
      241 tggccgtcag tgggtgacac tggcggcttt tttgtttttc tttactttca ttttctgtcg
      301 gcggtgacgg agacatacat cagatggaaa aaatcacaac aggtgtgtca tacaccacgt
      361 cagcggtggg gacggaatac tggttactgc agctgctgga caaagtctct ccgtcccagt
      421 gggtggcaat aggtgtgctg ggaagtctgc tgtttggcct gctgacgtat ctgactaacc
      481 tgtatttcaa aatcagagag gaccgtcgta aggtggcgcg gggagagtag tcgatgaata
      541 aacaatacga actggttgta aaatgaatat ttctaactga aaaaacgttc catgaggtaa
      601 gaaaaggtca caggcaatca ataacaggac gtgatgaaag acccttgcat ttgtgcgctt
      661 tctctttaga tagcagcaga tactgaaaat ctgagttgtc ggggagtcag ggatacagct
      721 gtgcaagagt tggtcattgt gattccattg aaatcctgta tgccatgaag ggcaggattt
      781 tatggctacc tgagctttgg tgatagtaag ttgaaaattc gcattttttg ctgacatgcg
      841 taacgagaat cccataagca gggaggactt aattcttcat taacccatgc gttgatatta
      901 tgtttcagcc gttgaagcat cagcggtgtt aatgttgtgg taataatatc cagcgtttta
      961 tgtgagatct taccgtaagg gtctgcaaga atgctgcttg ttgcttcgtt attatctgcc
     1021 atcagaagaa gtaactctga tttaacgttt tctgtcatta gttgtaaaaa tcttctgcgc
     1081 aaactttctt tactgttcat ttatatggct tcatttgttg taatctgctg cgtctcaagg
     1141 gatatgttta tgagagcgac catgagtgtt ggattatata cctaacatat caagggatta
     1201 gaaatcgata aatccccatg aacgaaaaaa taaaatacgg cctgtcggct gccgttctgg
     1261 cgctgattgg tgcaggtgct tctgcgcctg aaatcctcga ccagtttctg gatgaaaagg
     1321 aaggtaacca caccacggca taccgtgatg gtgcggggat ctggaccatc tgccgtggtg
     1381 ccattctggt ggatggtaag cctgttattc ctggcatgaa gctgtcaaag gaaaaatgcg
     1441 accgggttaa tgccatcgaa cgtgacaagg cgctggcatg ggtggagaaa aacatccggg
     1501 tgccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac aacattggcc
     1561 ccggtaagtg cttcccgtcg acgttttata aacgaattaa tgcaggcgat cgaaaaggtg
     1621 cctgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt atccgttcaa
     1681 ataattgcta cggtcaggtc tcacggcgtg accaggagag cgcgctggcg tgctgggaca
     1741 tcgacagata gcagaatatt ttcctgaaaa atgacgttgg ccaacgcggg tggataacac
     1801 gaaatcctga aaactggtaa aacctaagtg aataaaagta aaaaccccgt ttgttggcag
     1861 caagcggggt tttgtgtttt ctgaccttga gtaaggcaag ggagaaatta tgggtaggga
     1921 ggtactttcc ctgtgaggaa gtataaaaga ttctttctga ggttgtccat tatgaaaggc
     1981 attgaagtgg agacgccagc cagtctggat ttaacaagag cggcagcttt tgccattcgt
     2041 attgtggcca ttgctgttct ggtctgggca atccgttggt ggtgatatga accgtgttct
     2101 gtgtgtggtt atcattgtcc tgctggtggc ctgtggtgcg cttagtctgg ggctgaatca
     2161 ttaccgtgat cacgccatca tctacaaaga gcagcgcgat aaaaaagcca gtgagctgga
     2221 gctggcgaac gcgacaatta ctgatatgca gatacgccag cgtgatgtcg ctgcacttga
     2281 tgccagatac tcgagggaat tagccgatgc gagagctgaa aatgaaactc tgcgtgctga
     2341 tgttgccgct ggtcgtaagc gcctgcggat caacgccacc tgctccggta ccgtgcgtga
     2401 agccaccggc acctccggcg tggataatgc aaccggcccc cgactggcag acaccgctga
     2461 acgggat
//
LOCUS       DAZFDC010000284         2414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFDC010000284 DAZFDC010000000
VERSION     DAZFDC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.286"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..349)
                     /locus_tag="ACHJ48_004953"
     CDS             complement(<1..349)
                     /locus_tag="ACHJ48_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO4710303.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKLKFDSL"
     gene            complement(358..1125)
                     /gene="sctT"
                     /locus_tag="ACHJ48_004954"
                     /pseudo
     CDS             complement(358..1125)
                     /gene="sctT"
                     /locus_tag="ACHJ48_004954"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
     gene            complement(1127..1387)
                     /locus_tag="ACHJ48_004955"
     CDS             complement(1127..1387)
                     /locus_tag="ACHJ48_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4710304.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1398..2063)
                     /locus_tag="ACHJ48_004956"
     CDS             complement(1398..2063)
                     /locus_tag="ACHJ48_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO4710305.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2053..>2414)
                     /gene="sctQ"
                     /locus_tag="ACHJ48_004957"
     CDS             complement(2053..>2414)
                     /gene="sctQ"
                     /locus_tag="ACHJ48_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system cytoplasmic ring
                     protein SctQ"
                     /protein_id="HFO4710306.1"
                     /translation="YPEELKMADHFQYEEDFETDDFDIKKSESEIYDENDEQMINSFE
                     ELPVKIEFVLGKKIMNLYEIDELCAKRIISLLPESEKNIEIRVNGALTGYGELVEVDD
                     KLGVEIHSWLSGHNNVK"
BASE COUNT          827 a          506 c          370 g          711 t
ORIGIN      
        1 tcaatgaatc aaactttaat tttacagctt cagttgctaa ttgcatttta ctttgaaacc
       61 aggagacaag tattgttgtg aacatgcaaa ccagcaaaaa aggaatgatc gttttcaacc
      121 aaccaatccc catagcttta aaatagtccc aaatatccgg tttagcgtta tgatcaagga
      181 tatattcaag aatcgacata atgtgatgca catcaaaaac atatccgaga tataaagtac
      241 ccacaagcat tattacagaa acggttaagt ctctactttt tagaatttgc ccttttttag
      301 aagcatcctg cagtttcttt tgtgttggct tttcagtttt atttgccatg aaacactcta
      361 tttctcaatg tatagttgca attgttctgg gaaaaaggat aacggcatca ctcgttcagc
      421 cagtattggg ccaaaataaa taattaatat taaaaatgcc agcgcacttt tgacagtcag
      481 tgaaatggca aaagcattta gttgtgaagc atagcgtgcc agcaatccca ggacagcctc
      541 accacccagc ataacggcaa ttacggggct tgcgtaaacg atagtatgag tcattatatt
      601 attaatatat gaaaataaag gctctaattt aggaaaatta aaacttcctg aaggccataa
      661 attataactt tgctaaagag tttccagaat aaagttcaat ccaccgttag taagataaac
      721 cgctgcagag aaaaggttaa ataatcttgc taattccgag gtatcgacac ctgtagccgg
      781 atccaaagta ctacttaacg ttgcaccgcg ttggttatcg ataaagctac ctatagcaag
      841 gaaaatccaa aatggagatg ctaataagca agcaatgaac agccctatga taatttctct
      901 tgcagctaac aaagcgatat cgatttcatt taaggtcgat aattcatagt ggtaatgctg
      961 aactaatgca catgatacca ctattattat tggatgacgg actactgttg gtatattccc
     1021 actggcgaaa aatggtaaca aatagaaagt tggtgccaat cggcaaaaac cgagcgctgt
     1081 agcagccagt agactatgca actgatagag tatcacctcc cccatgctat cctctcgcaa
     1141 atgctaaatt cagcatttca ataccaaaag aatataattt ttcaccatac catccgctca
     1201 ttaaaaaaaa gcaaatactt acgcacaata atttcacgcc aaatggcaac gtctgttctt
     1261 gcaactgcgt taccgtttga aaaagcccta ccaataaacc aacaaatgtc gctactgcaa
     1321 taggtcctgc agacataact aaaatcagat acagcgccct attccctgca aaaacaatgt
     1381 catccatatt tttttcctca gggattaatg gataggtcaa aatattgtaa tatgagtcct
     1441 tttgataaca ttgtccaacc gtccatcgcg acaaacagaa ttaattttat cggtgtagat
     1501 atggttaccg gactcatcat catcatacct aacgttaaca gaacgctgga tataaccaga
     1561 tccactacaa caaagggtaa atagatataa aaacctataa taaatgcact ttttatctca
     1621 cttaacgcat atgccgggag taacgagaaa attgaaatat tatcatcatc gattatctct
     1681 tcattgtctt cagaactatt caccttctga atcttttcaa aaaagctaac caactccggt
     1741 tcagaatatt ttatcaaata tgatttataa ccactcatgc ctgtctcaac aaaattaaca
     1801 accgacgcta cattattgaa tgaaagattc tcattctgac tgttataata tatttccttc
     1861 ccaaccggca tcattacaaa cattgacaat agcaaagcaa ccccattaag tgtcatattt
     1921 gatggcactt gctgaagtcc caatgcgttt cggacaataa caaaaacaat cgagaattta
     1981 ataaaacatg tcccggatgc tattataaac ggcaatagtg taaataatga tagtatcgct
     2041 atcagcgaaa tgctatttga cattattatg ccctgataac caagaatgaa tctctacgcc
     2101 caatttatca tccacttcaa caagttcgcc atagccagtt agcgcgccat ttacacgtat
     2161 ctctatattt ttctcagatt caggaagcag agatattatt ctttttgcac agagctcgtc
     2221 tatttcatat agattcatta ttttcttacc aagaacaaac tcaattttta caggcaactc
     2281 ttcaaaacta ttaatcatct gttcgtcatt ttcatcataa atctcactct cgcttttttt
     2341 gatatcaaag tcatctgttt caaaatcttc ctcgtactga aaatgatcag ccatttttaa
     2401 ctcctctgga taaa
//
LOCUS       DAZFDC010000285         2378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFDC010000285 DAZFDC010000000
VERSION     DAZFDC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.287"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..430
                     /locus_tag="ACHJ48_004958"
     CDS             50..430
                     /locus_tag="ACHJ48_004958"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4710307.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            427..774
                     /gene="tnpB"
                     /locus_tag="ACHJ48_004959"
     CDS             427..774
                     /gene="tnpB"
                     /locus_tag="ACHJ48_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO4710308.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            824..2362
                     /locus_tag="ACHJ48_004960"
     CDS             824..2362
                     /locus_tag="ACHJ48_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO4710309.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT          552 a          657 c          693 g          476 t
ORIGIN      
        1 agacaaagtg gtgtccacca aataagtagt gggaaccaaa gtatcagata tgcagaaaaa
       61 tgtgactccc ggcaggcgaa aaggctgccc taattatcct cccgaattta aacagcagct
      121 cgttgctgcc tcctgtgaac ccgggatatc catctcaaaa cttgctcttg aaaatggcat
      181 taacgccaat ctgttgttca aatggcgaca acaatggcgc gagggaaagc tgctattacc
      241 ttcttcagag agcccccagc tacttcctgt gactctcgat gcagctgccg aacagccaga
      301 atcgctcgca gaggacccgg aaaccctcag tatcagctgt gaggtaacgt tccggcacgg
      361 gacgctccgc ttcaatggca atgtcagcga aaagctcctg actctgctga tacaggaact
      421 gaagcgatga tcccgttacc ttccgggacc aaaatttggc tggttgccgg tatcaccgat
      481 atgagaaatg gcttcaacgg cctggctgcg aaagtacaaa cggcgctgaa agacgatccc
      541 atgtccggcc atgttttcat tttccggggc cgcagcggca gtcaggttaa actgctgtgg
      601 tccaccggtg acggactgtg cctcctgacc aaacggctgg agcgtgggcg cttcgcctgg
      661 ccgtcagccc gtgatggcaa agtgttcctt acgcaggcgc agctggcgat gctgctggaa
      721 ggtatcgact ggcgacagcc taagcggctg ctgacctccc tgaccatgct gtaaatctct
      781 ttatcctggt tgtcacagaa taagcccggt aaaatacggg cttatgaacg acatctcttc
      841 tgacgacatc ttcctgctga aacagcgcct ggccgaacag gaagcgctga tccacgccct
      901 gcaggaaaag ctgagcaacc gggagcgcga aatagaccat ctgcaggcgc agctggataa
      961 actccgccgg atgaacttcg gcagtcgttc cgaaaaagtc tcccgccgta tcgcacaaat
     1021 ggaagccgat ctgaaccggc ttcagaaaga gagcgatacg ctgactggta gggtgtatga
     1081 cccggcagta cagcgtccgt tgcgtcagac ccgcacccgt aagccgttcc ctgaatcact
     1141 accccgtgac gaaaagcgac tgttgcctgc ggcgccgtgc tgcccgaact gcggcggttc
     1201 actgagctat ctgggcgagg ataccgccga acagctggag ttgatgcgta gcgccttccg
     1261 ggttatccgg acggtacggg aaaaacatgc ctgtactcag tgcgatgcca tcgtgcaggc
     1321 acctgcacct tcgcggccca tcgagcgggg tatcgccgga ccggggctgc tggcccgcgt
     1381 gctgacctcg aagtatgcag agcacacccc gctgtatcgc cagtcagaaa tatacggccg
     1441 gcaaggtgtg gagctgaggc gttcactgct gtcgggctgg gtggatgcat gctgccggct
     1501 gctgtctccg ctggaagagg cgcttcatgg ctatgtcatg actgacggca aactccatgc
     1561 cgatgatacc ccggtccagg tactgctgcc gggtaataag aagacgaaga ccgggcggtt
     1621 gtgggcgtat gttcgtgatg accgcaatgc agggtcagcg ttggcacctg cagtgtggtt
     1681 cgcttacagc ccggacagaa aaggcatcca tccgcagact catcttgcct gcttcagcgg
     1741 tgtgctgcaa gcggatgcgt acgccgggtt caacgagctg tatcgcaatg gtgggataac
     1801 ggaagctgcc tgctgggctc atgcccgccg aaagatccac gatgtgcacg tccgcatccc
     1861 gtcagcactg acggaagaag ccctggagca gatcggtcag ttgtacgcca tagaggcgga
     1921 tataagggga atgccggcag agcagcggct tgctgaacgt cagcgaaaaa cgaaaccgtt
     1981 gttgaaatcc ctggaaagct ggttgcgtga aaagatgaag accctgtcgc gacactcaga
     2041 gttggcgaag gcgttcgcgt acgcacttaa ccagtggccg gcactgacgt actatgcgaa
     2101 cgatggctgg gtggaaatcg acaacaacat cgctgaaaat gccctgcggg cggtcagtct
     2161 gggtcgtaaa aacttcctgt tcttcggctc tgatcatggt ggtgagcggg gagcgctact
     2221 gtacagcctg atcgggacgt gcaaactgaa tgacgtggat ccagaaagct accttcgcca
     2281 tgtgcttggc gtcatagcag actggccggt caaccgggtc agcgaactgc ttccgtggcg
     2341 catagcactg ccagctgaat aacacatccc cgtcaata
//
LOCUS       DAZFDC010000286         2374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFDC010000286 DAZFDC010000000
VERSION     DAZFDC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.288"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2236
                     /locus_tag="ACHJ48_004961"
     rRNA            <1..2236
                     /locus_tag="ACHJ48_004961"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          609 a          530 c          746 g          489 t
ORIGIN      
        1 agacccgaaa cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga
       61 ggaccgaacc gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc
      121 aatcaaaccg ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt
      181 catctccggg ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg
      241 caaactgcga ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt
      301 cgtgaagagg gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga
      361 aacgatgtgg gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag
      421 aaagcgtaat agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca
      481 tgcaccgaag ctgcggcagc gacactgtgt gttgttgggt aggggagcgt tctgtaagcc
      541 tgtgaaggtg tgctgtgagg catgctggag gtatcagaag tgcgaatgct gacataagta
      601 acgataaagc gggtgaaaag cccgctcgcc ggaagaccaa gggttcctgt ccaacgttaa
      661 tcggggcagg gtgagtcgac ccctaaggcg aggccgaaag gcgtagtcga tgggaaacag
      721 gttaatattc ctgtacttgg tgttactgcg aaggggggac ggagaaggct atgttggccg
      781 ggcgacggtt gtcccggttt aagcgtgtag gctggttttc caggcaaatc cggaaaatca
      841 aggctgaggc gtgatgacga ggcactacgg tgctgaagca acaaatgccc tgcttccagg
      901 aaaagcctct aagcatcagg taacatcaaa tcgtacccca aaccgacaca ggtggtcagg
      961 tagagaatac caaggcgctt gagagaactc gggtgaagga actaggcaaa atggtgccgt
     1021 aacttcggga gaaggcacgc tgatatgtag gtgaagtccc tcgcggatgg agctgaaatc
     1081 agtcgaagat accagctggc tgcaactgtt tattaaaaac acagcactgt gcaaacacga
     1141 aagtggacgt atacggtgtg acgcctgccc ggtgccggaa ggttaattga tggggtcagc
     1201 gcaagcgaag ctcttgatcg aagccccggt aaacggcggc cgtaactata acggtcctaa
     1261 ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa tgatggccag
     1321 gctgtctcca cccgagactc agtgaaattg aactcgctgt gaagatgcag tgtacccgcg
     1381 gcaagacgga aagaccccgt gaacctttac tatagcttga cactgaacat tgagccttga
     1441 tgtgtaggat aggtgggagg ctttgaagtg tggacgccag tctgcatgga gccgaccttg
     1501 aaataccacc ctttaatgtt tgatgttcta acgtggaccc gtgatccggg ttgcggacag
     1561 tgtctggtgg gtagtttgac tggggcggtc tcctcctaaa gagtaacgga ggagcacgaa
     1621 ggttggctaa tcctggtcgg acatcaggag gttagtgcaa tggcataagc cagcttgact
     1681 gcgagcgtga cggcgcgagc aggtgcgaaa gcaggtcata gtgatccggt ggttctgaat
     1741 ggaagggcca tcgctcaacg gataaaaggt actccgggga taacaggctg ataccgccca
     1801 agagttcata tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca tcctggggct
     1861 gaagtaggtc ccaagggtat ggctgttcgc catttaaagt ggtacgcgag ctgggtttag
     1921 aacgtcgtga gacagttcgg tccctatctg ccgtgggcgc tggagaactg aggggggctg
     1981 ctcctagtac gagaggaccg gagtggacgc atcactggtg ttcgggttgt catgccaatg
     2041 gcactgcccg gtagctaaat gcggaagaga taagtgctga aagcatctaa gcacgaaact
     2101 tgccccgaga tgagttctcc ctgacccttt aagggtcctg aaggaacgtt gaagacgacg
     2161 acgttgatag gccgggtgtg taagcgcagc gatgcgttga gctaaccggt actaatgaac
     2221 cgtgaggctt aaccttacaa cgccgaaggt gttttggcgg atgagagaag attttcggcc
     2281 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggcc
     2341 ttagcgcggt ggtcccacct gaccccatgc cgaa
//
LOCUS       DAZFDC010000287         2353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFDC010000287 DAZFDC010000000
VERSION     DAZFDC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.289"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(168..893)
                     /gene="fimC"
                     /locus_tag="ACHJ48_004962"
     CDS             complement(168..893)
                     /gene="fimC"
                     /locus_tag="ACHJ48_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO4710310.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     IIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(930..1469)
                     /locus_tag="ACHJ48_004963"
     CDS             complement(930..1469)
                     /locus_tag="ACHJ48_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4710311.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTPPGGNMQFQGVIIAETCL
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(1534..2082)
                     /gene="fimA"
                     /locus_tag="ACHJ48_004964"
     CDS             complement(1534..2082)
                     /gene="fimA"
                     /locus_tag="ACHJ48_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO4710312.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQDGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          603 a          591 c          508 g          651 t
ORIGIN      
        1 caggcgacaa agagccggac aaaaaaacca gccaaacgat gcttacgaat atgcaagcat
       61 tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc ggtttaaaat
      121 cacccggcag tcaattcttt ttattcaggc gaaaaattcc ccctgcgtta ttccattacg
      181 cccgtcattt tgggggtaag tgcaccataa tcatttattg ttcggtaagt aatattgctt
      241 cccgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa ggcattttca
      301 agaacccggg ctccggcatt caactctgtt accgtcaggt aatagggtgt cgggttaatc
      361 agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg atcgggtggc
      421 aacgctaatt tagccggacg atagtacagt ttaatgcggc tgataattgc gagctgtagc
      481 gtattctcag tcaattttga tttatccatt gacggaatcg ctttaacgtt catccagaat
      541 aaactttccc ggtcctgtgg caattggtta tttgttgcat caagaatacg taaggtattc
      601 tctttttttc ccttcatcgc aaacagagga ggcgtcacga taaaacgacc atcctttaca
      661 ccatcggcat tttccaccca tgattgaatt aaataggtac tattttcatc attatttgtc
      721 acggcaagtt gcacttgttt ttgccctgcc ggataaatta tgcgagtcgc acctaaggcc
      781 actcccgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat acctgccagc
      841 aagcagaatg ttatttcctg cgatttcctt acattgacgt ttttattact cactgtcctg
      901 ttcctgttat cacattatct gctgaacaat tactgatagg ttaaagagaa ccaggcctgt
      961 gcattggcga tgccgccagt aacccgacgc ccggtagcac gatatttggc gatgaaatgt
     1021 agcgaagtag agcctgagta aagccgtttc cagtttgctg gaggacgatt aatcggtacg
     1081 aggtttcctt catcatcaaa caacgctacg ccaatattgg tggctatccc tggcccctct
     1141 cccacggaaa gcacatccgg atttttacca tccgcgacac cgtgaaacgc cacacccaca
     1201 cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac cggtgcgcta
     1261 tcttccccta ccgcatgaaa ccggttactg ctgatttgcc ccatattgac cgtcatttgt
     1321 ttatcaccgg cttcaatcag gcaagtttcc gcaataatga cgccctgaaa ttgcatattt
     1381 ccgccgggcg gcgtggtatt ccatttattt ccggccagag caaacattgg cagcaacgac
     1441 gctaatagaa ataatctctt ccttttcatc gttattttta tcgcacaagg gtgggcatcc
     1501 ctgcccgtaa tgacgtccct gaacctgggt aggttattga tactgaacct tgaaggtcgc
     1561 atccgcatta gcagcacccg gggttgcctc gccgattgca taataacgcg cctggaacgg
     1621 aatggtgtta gtaccgttat tcagggttgt ttgctcactg aatgtcgcac catccagcgt
     1681 cagcgcagca cccgttctgt ccaggatctg cacaccaacg tttgttgcgc tacccgcagc
     1741 tgaactctgc agagccagaa cgttggtatg acccgcatca atcgccgtac ctaaaaaggc
     1801 aacagcggct ttagatgcaa cattggtatc gcaatcattc agctgaatgt taaaaccgac
     1861 agcagaactg gttgctccgt cctgtgccag cgatgcggta cgaacctgtc ctaactgaac
     1921 ggtttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt cccctttaaa
     1981 gtgaacggtc ccaccattaa ccgtcgtggc agcggccaga gccgctgtag aactgaggga
     2041 cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt cctttcaaaa
     2101 aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc aacgagtttg
     2161 actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca ttttgactca
     2221 tagaggaaag catcgcgcac aaactttttc agtttatttg ctggattaat attctattgt
     2281 tatctttatt tatagatttt tatattgcat gaggtggttt ttggagagaa gaatgaggaa
     2341 gatgcgtcga gcc
//
LOCUS       DAZFDC010000288         2333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFDC010000288 DAZFDC010000000
VERSION     DAZFDC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.290"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..207)
                     /locus_tag="ACHJ48_004965"
     CDS             complement(1..207)
                     /locus_tag="ACHJ48_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4710313.1"
                     /translation="MNQHNTRLIRLPEVLHRTGYGKAWIYHLINQNRFPSPVKIGARS
                     VAFIESEVDEWIQLTIENSRKHVA"
     gene            complement(307..1158)
                     /locus_tag="ACHJ48_004966"
     CDS             complement(307..1158)
                     /locus_tag="ACHJ48_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFO4710314.1"
                     /translation="MKNWSVEHTREVKKWLDLDTYRGFEELPLIHLYHELLARTLFFK
                     PYHEEFEAEAVRLYIDRIFTGKPFLITEKHLGYLTREDTLYQPPHFFLTTTERLAQLS
                     IVGLRNSLFFWDGSDEYSVNREFLDSPVSEVLPKLFRDTVMVEIDLANGTDEEIAESL
                     KAALPQWRKVRGIEPDVTEAIRFGYGTIKKIINYRLLPMIDILVWSKLYKVRISEDRL
                     SRLLYTDDDDEINTRLNHQIRDTDKPLALKAASIPFIRQFNLFINKNSHLKKIRVSDV
                     MKIADSD"
     gene            complement(1585..>2333)
                     /locus_tag="ACHJ48_004967"
     CDS             complement(1585..>2333)
                     /locus_tag="ACHJ48_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO4710315.1"
                     /translation="DAGAGFEQTLTGMTVGIDSPNDIPEGIATLGAFMGYSHSHIGFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMESKSVDTRSIYRELGATLS
                     YNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSS
                     TLSGHLGVGYSHGAGVESPWNAVAGVNWSF"
BASE COUNT          635 a          558 c          514 g          626 t
ORIGIN      
        1 tcaggcaaca tgctttcttg aattttcgat agttaattga atccattcgt ctacttcact
       61 ttcaatgaaa gcaacagagc gcgcaccgat tttaacgggg gaaggaaaac gattttggtt
      121 gatgaggtga tagatccaag ctttaccgta tccagttcga tgcagaacct ctggtaaacg
      181 aataaggcgg gtgttgtgct gattcatagt cgtttcctct gtgccgtttt gatggaaatg
      241 actatacaaa tgaatcagca ggaaaaagac cgaagctggg aacgccagag ctaatggagg
      301 ttggtcttag tcagaatcag cgattttcat cacgtctgaa actctgatct ttttcaaatg
      361 gctgttcttg ttgatgaaca gattaaactg gcgaataaaa ggtatactcg ccgccttcaa
      421 agcaagaggt ttgtctgtat ccctgatttg gtggttcagg cgagtgttga tctcatcatc
      481 gtcatctgta tacaacaggc gagaaaggcg gtcttctgat atacgtactt tatataattt
      541 tgaccatacc agaatgtcga tcatgggaag gagtctgtaa ttaatgattt tcttaatcgt
      601 tccataacca aagcgaatag cttccgttac atctggttct atgccacgca ctttacgcca
      661 ttgaggaagt gcagccttta gggattcagc gatctcttca tctgtcccgt ttgctaggtc
      721 gatttcgacc atgacagtat ccctgaacaa ttttggcaaa acttccgata cagggctatc
      781 gaggaattct ctgttaacag aatactcatc gcttccatcc caaaaaaaca gtgagttccg
      841 taatccaact atgctgagtt gagccagtct ttcggttgtc gtcaaaaaga agtgcggggg
      901 ctggtaaaga gtatcctctc ttgtgagata gccgagatgc ttttctgtga tgagaaaggg
      961 ctttccagtg aagatcctgt cgatgtagag ccttacggct tctgcttcaa attcttcatg
     1021 atatggtttg aagaacaatg tccttgccag caattcatgg tagaggtgta taagcggaag
     1081 ctcttcgaat ccgcgatatg tatctaaatc cagccacttc ttcacctctc tcgtatgttc
     1141 aactgaccag tttttcataa cgatctccaa caggcattag acttaatgta tgtagcaaac
     1201 gtaatcgaca tgttaaagga agaccaatga aatgagttca gatgccatca ggaagttgaa
     1261 ccaaaaggtt tgatattttt tgatacagtt cgctaacgga ctattttcag gggtttatgc
     1321 cgttacgatt tatggttcct ttatagaacc aaaggtaaag tcagaaacag cagttatcta
     1381 gaaaattcaa tcaaatagat atctagttac agggacgcca gccaatcatg attggacggt
     1441 gtaaggacaa caccaacaaa aacaggaagt tagaagtctc agcaggacac cgaccagacg
     1501 gtgaggagac ataaaaggat acgcaaagga gccgcggctc ctggtgacat gaaagcccac
     1561 agatgtgggc tttttcgttg atggtcagaa cgaccagttc acaccagcta ccgcgttcca
     1621 cggggattcc acaccggcac catggctata ccccacccca agatgcccgc ttaacgtact
     1681 gctgaatgag gctttaatac ctgcctggta tattccacgt ctgcccgaca aatcattgac
     1741 gaaattaccg tcattattca ccttcacccg gttatcatcg acaaattctt tgcgcacagc
     1801 cgccttcagc cacggctcaa tttccatacc gttccccaga cgcatgttgt aactcagcgt
     1861 tgcgcccagt tcacgatata tactgcgggt atcgactgat ttcgattcca tgccattgga
     1921 taaatgatat tcggggttat cagcggtgaa ccccgttaac gatgcatacg gcgtcaggtt
     1981 ccagttacca tcggtaaatc gcatcccggt ttcaatgtga ccgcccagcc cgttgctgtg
     2041 gtaactgcca ttggcggctc caccgctgct cattttaccg gctacgttac tttcaaaacg
     2101 gttcagcttc acgacaccgt ccagatagaa accactttca tgttcccaac tggcatagcc
     2161 gcccagagaa taactgccca cactgccatg tcctccgcga tcaaaaccga tatgtgaatg
     2221 ggaataaccc ataaaagcgc ccagcgtcgc aatcccctca ggaatatcat tagggctgtc
     2281 gatccccact gtcattccgg tcagcgtctg ctcaaacccg gcccccgcat cgg
//
LOCUS       DAZFDC010000289         2318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFDC010000289 DAZFDC010000000
VERSION     DAZFDC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.291"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..577
                     /locus_tag="ACHJ48_004968"
     CDS             <1..577
                     /locus_tag="ACHJ48_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710316.1"
                     /translation="FSLTSDGKLTAKNADISGSVNANSGTLNNVTVNENCTIKGMLEA
                     TQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAY
                     SDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEF
                     KVFHKGNQRAGNITDCTVIVTKKAASGISIR"
     gene            648..1247
                     /locus_tag="ACHJ48_004969"
     CDS             648..1247
                     /locus_tag="ACHJ48_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4710317.1"
                     /translation="MRKVCAAILSAAICLAVSGVPAWASEHQSTLSAGYLHASTDAPG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDYVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYFRVTDNKRKTHDVLTGSDDARYSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1307..>2318
                     /locus_tag="ACHJ48_004970"
     CDS             1307..>2318
                     /locus_tag="ACHJ48_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4710318.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENP
                     DEAGRYSMDVEYGQYSVTLLVEGFPPSHAGTISVYEDSQPGTLNDFLGAMTEDDARPE
                     ALRRFEQMVEEAARHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASESA
                     RQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNAARDATT
                     ATEKARESAESAQSAEQSRIAAEDAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERG
                     DTGPAGATGERGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTGATGPAGP
                     QGP"
BASE COUNT          617 a          536 c          718 g          447 t
ORIGIN      
        1 attttccctg acatcagacg gaaagctgac cgctaaaaat gcggatatca gtggcagtgt
       61 gaatgcgaac tccgggacgc tcaacaacgt cacggtaaat gaaaactgta cgattaaggg
      121 catgctggag gcgactcagg tcagaggtga cttcgttaaa gctgtatcca aatcatttcc
      181 gaaacaggct ggtacgtggg gtaacacgga aacaccaaac gggacggtta cagtcaccat
      241 cagcgatgat cataactttg accgtcaaat cattattccg cccattatct ttaacggaat
      301 agcgtatagc gatccgggaa gtggtaataa cccgggaggt acaagataca cgggttatgg
      361 ttttgaagtt cgcaaaaacg gtgtattaat cgcatccaga gaaactaaag gggccattcc
      421 cggtagctac agtgcggtta ttgatatgcc gagtggcagg ggaagcgtca ctctggagtt
      481 taaggttttc cataaaggca atcagcgggc aggtaatatc accgactgta cggtgattgt
      541 gaccaaaaaa gcggcttccg gcatcagtat ccgttgaaat atttataacc ctaataacgg
      601 gcgccagaaa tggcgccttt tttattgcag aaaatcggga ggtaattatg cgtaaagttt
      661 gtgcagcaat tttgtccgca gccatttgtc tggccgtatc cggtgtgcct gcatgggcgt
      721 ctgagcatca gtccacgctg agcgcaggat atcttcatgc cagtacggac gctcccggca
      781 gcgatgatct gaacgggatt aacgtgaaat accgttatga gtttacggac acactggggc
      841 tgattacgtc cttcagttat gccaacgccg aagatgagca aaaaacgcat tacagcgata
      901 cccgctggca tgaggattac gtgcgtaacc gctggttcag cgtgatggca gggccgtctg
      961 tgcgtgtgaa tgaatggttc agtgcgtatg cgatggcggg tgtggcttac agccgtgtgt
     1021 cgactttttc cggggattat ttccgcgtaa ctgacaacaa gaggaaaacg cacgacgtgc
     1081 tgaccggaag tgatgacgct cgctacagca acacatctct ggcgtggggg gctggcgtgc
     1141 agtttaaccc gaccgaatca gtggccgttg atgtcgctta tgaaggctcc ggcagcggtg
     1201 actggcgcac tgacggtttc atcgtgggtg tcggttataa attttgatta gtaacacagc
     1261 gttatgacag cccgccggtt caggcgggct tttttgtgga gtgggtatgg cagcagtaca
     1321 aatatcaggc gtgctgaaag atggtgcggg aaaaccaata cagaactgca ccattcaact
     1381 gaaagccaga cgtaacagca ccacggtggt ggtgaacacg gtggcctctg aaaatccgga
     1441 tgaggcaggg cgttacagca tggacgtcga gtatggtcag tacagcgtca ctctgttggt
     1501 ggagggattc ccgccatcac atgccgggac catctccgtg tatgaagatt ctcaaccggg
     1561 tacgctgaat gattttctcg gtgccatgac ggaggatgat gcccgtccgg aggcactgcg
     1621 tcgttttgaa cagatggtgg aagaagcagc gcgtcacgca gaggaggcga agaagaatgc
     1681 cggagaagca gagacgtccg cgaggaatgc cggcatatca gccagtaagg cggaagcgag
     1741 cgccgcaaat gctgatactt cagcagagga tgcatcggag tcagcccggc aggcggcaga
     1801 aagtgcagcc tctgcaaaga agtcagagga agcgtcctcg tcctcagcct ctgaggccgc
     1861 tcaaaaagcc agtgagtcat tacaaagtgc aacagatgcc gagttgtcaa aaaagacggc
     1921 agaaagtgca gccggtaatg cagccaggga tgcaacgacc gcaacagaaa aagcccggga
     1981 atcagcagaa agcgcacagt cagcggaaca aagcagaata gcggcggaag acgccgtaaa
     2041 cagaattccc accgtggtgg ggcctcccgg accaaagggg gaaccgggtc ccgcgggtcc
     2101 tcaggggccg aagggagata aaggagagcg tggagacacc ggtccggcag gggcaaccgg
     2161 tgaacgggga ccgggaggag atacaggtcc ggcaggtccg caggggccga aaggcgacag
     2221 gggagagcgg ggagagaccg gtctgacagg aagtacaggt ccacaggggc caaagggaga
     2281 taccggggca acaggtccgg caggaccgca gggaccga
//
LOCUS       DAZFDC010000290         2317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFDC010000290 DAZFDC010000000
VERSION     DAZFDC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.292"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..762
                     /locus_tag="ACHJ48_004971"
     CDS             <1..762
                     /locus_tag="ACHJ48_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4710319.1"
                     /translation="LISAEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLHGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDL
                     IHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFS
                     CNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDL
                     MAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
     gene            774..1235
                     /gene="yibG"
                     /locus_tag="ACHJ48_004972"
     CDS             774..1235
                     /gene="yibG"
                     /locus_tag="ACHJ48_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4710320.1"
                     /translation="MKACLLLFFYFSFICQLHGADVKIKQNESMMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIYKQLRYLEKSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            1870..2317
                     /locus_tag="ACHJ48_004973"
                     /pseudo
     CDS             1870..2317
                     /locus_tag="ACHJ48_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
BASE COUNT          779 a          394 c          489 g          655 t
ORIGIN      
        1 cttatcagtg cggaaggggc aacagaatgg tgcgcagaat acgatgaatg gggcaacctg
       61 ctgaatgaag agaacccgca tcagctgcag cagcttatcc gcctgccggg gcagcagtat
      121 gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct gcacgggcga
      181 tatatcactc aggatccgat tggactgaag gggggatgga atttttatca gtatccgttg
      241 aatccggtca taaatgtaga tccgcaaggt ttggttgata taaatttata ccccgaaagt
      301 gatcttatcc attctgtagc tgatgagatt aatatcccag gcgttttcac aatcgggggg
      361 catggtaccc ccacatctat tgaatccgca acgcgcagta tcatgacagc taaagatcta
      421 gcatatctaa ttaaatttga tgggaattat aaagatggga tgacagtttg gttattttct
      481 tgtaatacag gtaaaggaca aaattcattt gctagccaat tagctaaaga gttacataca
      541 aatgtaatag gacctgacac gctatggacg tggtgggggc gaggaactaa tggtaagtta
      601 aaaatggata cagtgctaac agcaccaacg aaccttaatt caaataagga tctaatggct
      661 ataacaacaa aagaccttgg taattggata acatatgggc catctgggca ccccatttct
      721 aatatgcaag gtacgccaga aaaacccagt gatataagat aggttgtaga tgtatgaaag
      781 catgcttgtt actatttttt tatttctctt ttatttgtca attgcatggt gctgatgtga
      841 aaataaaaca aaacgaaagt atgatggggt ctacagcaat gacctatgat ttaagtgaag
      901 aaaagttgat gaaactgaag tataaatcac aacatggtga ttcagaggca tcatttcgac
      961 tatatcaata ttactgcttt actaaaaata acatttacaa acaattgcga tacttagaaa
     1021 aatcagcatc tcaggggaat gttacagcgc agtttaatta tggggtcttt ttatcagata
     1081 caaatccaac attatcagaa tattataatt tgaatagagc tatttattgg atggaattcg
     1141 ctgtaaataa cggtaatatt gatgcaaaga gcaaacttca agagctcaaa aagctaaagc
     1201 gaatggatag aaggaagaat aaggagaatc cgtaaaagca atagttcatc agactgccgc
     1261 agcagtagta tgatgatgaa tcagggctgt actataacta ttatcggtaa cattaaccgc
     1321 agcaggggcg gtataacact caggctccga ttgggctgga aggtaggtgg aatttttatc
     1381 agtatccgtt gaacccgatt tctggaatcg atccccttgg gattagccac ctgtttatac
     1441 agcataactt taggtatgct ttcgtgtgtt tcagatatac catgtgatga taatttttat
     1501 gatgtgctta gtattccagt cgcttctggt aataatatgc aatgtaaaaa taatccccgt
     1561 tgtacacatt tataaataga tggccaatat ctcaaggcct atggagttgg aatgttaatg
     1621 acccgggagc aagcaataga aaacctgacg gcatacgatt agtaccgtca gttaatacag
     1681 gaacttataa tcgcaatgga ttttcaatac atagttgttt aaacgctttt ggtccgtctt
     1741 taggacctcg attctgttcc gagggttgta taactggttt atctaatgac atgcaaaaat
     1801 taaatgagtt aatattttca gagccagata gcacattaac agttacggac taaaaaatat
     1861 ggcaaggtta ttaacaattg tatttttttc tctatttctg cgagtgcagt tactgcaaga
     1921 caatttcaga gagagttaga tttggcagac aaagcaatat tatgggcact aatatcagcc
     1981 agtactaaag agggacgaaa agcgtgttca ctaagctatt ttgcctgtaa ggccgcagag
     2041 gctgaactcg gactggcata tatggctgcg aatgataaca aggaatttct cacatcctta
     2101 tctaatataa tgagatacaa aatagatgct ggactttccg aatcctatac atgctattta
     2161 ttaagtaagg gaaagatcat aagaccatat ctgaaaaatc taaatccact ccaacttgcc
     2221 gctgattgca ttgaaacagt aaataaaata aaagataaga ataaaaaaaa tcattgatat
     2281 aaattcagtt aatatttgta gtgatgataa aaatata
//
LOCUS       DAZFDC010000291         2279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFDC010000291 DAZFDC010000000
VERSION     DAZFDC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.293"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            321..866
                     /locus_tag="ACHJ48_004974"
     CDS             321..866
                     /locus_tag="ACHJ48_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO4710321.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            841..>2279
                     /locus_tag="ACHJ48_004975"
     CDS             841..>2279
                     /locus_tag="ACHJ48_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4710322.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSK"
BASE COUNT          551 a          538 c          655 g          535 t
ORIGIN      
        1 gatcaattaa tacgatacct gcgtcataat tgattatttg acgtggtttg atggcgtaga
       61 tgcacgttgt gacatgtaga tgataattat tatcattttg cgggtccttt ccggcgatcc
      121 gacaggttac ggggcggcga cctcgcggtt tttcactatt tatgaaaatt ttccggttta
      181 aggcgtttcc gttcttcttc gccgtaacct aatgttttta tttaaaacac cccctgaaaa
      241 gaaaggaaac gacaggtgct gaaaacgggc tttttggcct ctgtcgtttc ctttctctgt
      301 ttttgtccgt ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggcg
      361 cgagtatccg taccattcag aactggcagg agcagggaat gcccgttctg cggggtggtg
      421 gcaagggtaa tgaggtactt tatgactctg ccgccgccat aaaatggtat gccgaaaggg
      481 atgctgaaat tgagaacgaa aagctgcgcc gggaagttga agaactgagg caggacagcg
      541 agacagatct ccagccaggg actattgagt acgaacgcca tcgacttacg cgtgcgcagg
      601 ccgacgcaca ggaactgaag aatgccagag actcagctga agtggtggaa accgcattct
      661 gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggatccccc
      721 tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg
      781 atatcatcaa agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga
      841 gtgaatatat cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc
      901 tcactgttca ggccggagcc acagaccgcc gttgaatggg cggatgccag ttactatctc
      961 ccaaaagaat ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc
     1021 atgaatgcga tgggcagtga ctacatccgc gaggtgaatg tggtgaagtc tgcccgtgtt
     1081 ggttattcca aaatgctgct gggtgtttat gcctacttca tagagcataa gcagcgcaac
     1141 acccttatct ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag
     1201 ccgactattc gtgatattcc gtcgctgctg gcgctggccc cgtggtatgg caaaaagcac
     1261 cgggataaca cgctcaccat gaagcgtttc accaatgggc gtggcttctg gtgcctgggc
     1321 ggtaaagcgg caaaaaacta ccgtgaaaag tcagtggatg tggcgggtta tgatgaactt
     1381 gctgcctttg atgaggatat tgaacaggaa ggctctccga cgttcctggg cgataagcgt
     1441 attgaaggct cggtctggcc aaagtccatc cgtggctcca cgcccaaagt gagaggcacc
     1501 tgccagattg agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc
     1561 ccgcactgcg gggaggagca gtaccttaaa tttggcgata aagagacgcc gtttggcctc
     1621 aaatggacgc cggatgatcc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc
     1681 atccgtcagc aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc
     1741 tggacccgtg atggcattct ctggttttcg tcatccggtg aagagattga accgcctgac
     1801 agtgtgacct ttcacatctg gacggcgtac agcccgttca ccacctgggt tcagattgtc
     1861 aaagactgga tgaagacgaa aggggatacg ggaaaacgta aaaccttcgt gaacaccacg
     1921 ctcggtgaga catgggaagc gaagatcggc gaacgtccgg atgctgaagt gatggcagag
     1981 cggaaagagc attattcagc gtccgttcct gaccgtgtgg cttacctgac cgccggtatc
     2041 gactcccagc tggatcgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc
     2101 tggctgattg atcggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt
     2161 gtggatgagg ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcggtatcc
     2221 cgtatctgct gggatactgg cgggattgac ccgactattg tgtatgaacg ctcgaaaaa
//
LOCUS       DAZFDC010000292         2259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFDC010000292 DAZFDC010000000
VERSION     DAZFDC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.294"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..931
                     /locus_tag="ACHJ48_004976"
     CDS             <1..931
                     /locus_tag="ACHJ48_004976"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFO4710323.1"
                     /translation="ENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYL
                     EELRKFRPDIYNVCEQVASSSYIDLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCP
                     VDIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSESALVAAIGIEDMVI
                     VQTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVK
                     KIIVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLEN
                     PGIIPLNLIEVSSGDYLGEDDIIRQKERYKHED"
     gene            935..>2259
                     /gene="cpsG"
                     /locus_tag="ACHJ48_004977"
                     /gene_synonym="manB"
     CDS             935..>2259
                     /gene="cpsG"
                     /locus_tag="ACHJ48_004977"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO4710324.1"
                     /translation="MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKL"
BASE COUNT          622 a          493 c          574 g          570 t
ORIGIN      
        1 tgaaaatact ggtttttatt atgtaaataa gtttgtcgaa aagcctaatc gtgaaaccgc
       61 agaattgtat atgacttctg gtaatcacta ttggaatagt ggaatattca tgtttaaggc
      121 atctgtttat cttgaggaat tgagaaaatt tagacctgac atttacaatg tttgtgaaca
      181 ggttgcctca tcctcataca ttgatctaga ttttattcga ttatcaaaag aacaatttca
      241 agattgtcct gctgaatcta ttgattttgc tgtaatggaa aaaacagaaa aatgtgttgt
      301 atgccctgtt gatattggtt ggagtgacgt tggatcttgg caatcgttat gggacattag
      361 tctaaaatcg aaaacaggag atgtatgtaa aggtgatata ttaacctatg atactaagaa
      421 taattatatc tactctgagt cagcgttggt agccgccatt ggaattgaag atatggttat
      481 cgtgcaaact aaagatgccg ttcttgtgtc taaaaagagt gatgtacagc atgtaaaaaa
      541 aatagtcgaa atgcttaaat tgcagcaacg tacagagtat attagtcatc gtgaagtttt
      601 ccgaccatgg ggaaaatttg attcgattga ccaaggtgag cgatacaaag tcaagaaaat
      661 tattgtgaaa cctggtgagg ggctttcttt aaggatgcat caccatcgtt ctgaacattg
      721 gatcgtgctt tctggtacag caaaagtaac ccttggcgat aaaactaaac tagtcaccgc
      781 aaatgaatcg atatacattc cccttggcgc agcgtatagt cttgagaatc cgggcataat
      841 ccctcttaat cttattgaag tcagttcagg ggattatttg ggagaggatg atattataag
      901 acagaaagaa cgttacaaac atgaagatta acatatgaaa tctttaacct gctttaaagc
      961 ctatgatatt cgcgggaaat taggcgaaga actgaatgaa gatattgcct ggcgcattgg
     1021 gcgtgcctat ggcgaatttc tcaaaccgaa aaccattgtt ttaggcggtg atgtccgcct
     1081 caccagcgaa gcgttaaaac tggcgcttgc gaaaggttta caggatgcgg gcgtcgatgt
     1141 gctggatatc ggtatgtccg gcaccgaaga gatctatttc gccacgttcc atctcggagt
     1201 ggatggcggc atcgaagtta ccgccagcca taacccgatg gattacaacg gcatgaagct
     1261 ggtgcgcgaa ggggctcgcc cgatcagcgg tgataccgga ctgcgcgatg tccagcgtct
     1321 ggcagaagcc aatgacttcc ctcctgtcga tgaaaccaaa cgtggtcgct atcagcaaat
     1381 caatctgcgt gacgcttacg ttgatcacct gttcggttat atcaacgtca aaaacctcac
     1441 gccgctcaag ctggtgatca actccgggaa cggcgcagcg ggtccggtgg tggacgccat
     1501 tgaagcccga tttaaagccc tcggcgcacc ggtggaatta atcaaagtac acaacacgcc
     1561 ggacggcaat ttccccaacg gtattcctaa cccgctgctg ccggaatgcc gcgacgacac
     1621 ccgtaatgcg gtcatcaaac acggcgcgga tatgggcatt gcctttgatg gcgattttga
     1681 ccgctgtttc ctgtttgacg aaaaagggca gtttatcgag ggctactaca ttgtcggcct
     1741 gctggcagaa gcgttcctcg aaaaaaatcc cggcgcgaag atcatccacg atccacgtct
     1801 ctcctggaac accgttgatg tggtgactgc cgcaggcggc accccggtaa tgtcgaaaac
     1861 cggacacgcc tttattaaag aacgtatgcg caaggaagac gccatctacg gtggcgaaat
     1921 gagcgctcac cattacttcc gtgatttcgc ttactgcgac agcggcatga tcccgtggct
     1981 gctggtcgcc gaactggtgt gcctgaaagg aaaaacgctg ggcgaaatgg tgcgcgaccg
     2041 gatggcggcg tttccggcaa gcggtgagat caacagcaaa ctggcgcaac ccgttgaggc
     2101 aattaatcgc gtggaacagc attttagccg cgaggcgctg gcggtggatc gcaccgatgg
     2161 catcagcatg acctttgccg actggcgctt taacctgcgc tcctccaaca ccgaaccggt
     2221 ggtgcggttg aatgtggaat cacgcggtga tgtaaagct
//
LOCUS       DAZFDC010000293         2188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFDC010000293 DAZFDC010000000
VERSION     DAZFDC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.295"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(708..1223)
                     /locus_tag="ACHJ48_004978"
     CDS             complement(708..1223)
                     /locus_tag="ACHJ48_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO4710325.1"
                     /translation="MDNSKHSDEAEKLLAELSARKGEGEGEGEPKSTVSVFYLQPEEV
                     NTLSHQAKRGDGEAGFRLFLYYKLSNYDEEKSSQWLKIAANNGHATAQYRLYVELQEQ
                     GETKEANEWLQKIKLAASNNDSSAIWFLVNCYKNQGNKKEALQWAYKLKASKQPLDAD
                     RLIEELTKEQK"
     gene            complement(1292..>2188)
                     /locus_tag="ACHJ48_004979"
     CDS             complement(1292..>2188)
                     /locus_tag="ACHJ48_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4710326.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGL
                     YYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPLGLALDLNYYSPSDPI
                     YKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLASDIRANPKYHEGMPVV
                     LFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIMGQTARITIGGKDNGV
                     FELVPDEKQPGDFHKFTPTGSK"
BASE COUNT          601 a          510 c          387 g          690 t
ORIGIN      
        1 tgtaagctgt gaaaaacagt tttagataat aaggaatatc tcaattattg aacatttagt
       61 gcgaattatt tagtacaaaa aagcggcgtt aggtgatctt tccctgaggt gaggagatat
      121 taacatggac aaaaacaaga aacttatgac agaagaaata taaggaatta ttatacaagc
      181 tattaaatca acaataaaag atcttttaaa aaataacaaa aaacagaaca ctatcaaaac
      241 ataaaaagca aacaaaaaac aataagcatg ttgattgaat attttaaata catgttcttt
      301 tacaataaaa aaatgaatgg ttatcgaaca agccagcaat aataacctta ttattttcat
      361 aagctccatc cactaaaatg ctggagatat aaaaaatgaa ccaaacggct tctttggccc
      421 atacttaggt ttggggtgta gaatcttagt cataagatct atgccgagtc atgtgctggc
      481 cacatactta atgatacaaa aagctatatt ttcatctttt ctcttgcgac ttttgatgca
      541 tctgccaagt acatcgtaat gataaccggc gacgaagatg ccagttttgg tttcaacttg
      601 cgctttgatg agcttgttgc agtcgtcgta aacgtaattt tgtgtgtagg tgttgtagcg
      661 actggtaaag tttttctact ctccattttt ctgttacctg tttaaagcta cttttgttct
      721 ttcgttaatt cttcaattag tcgatctgca tcaagcggct gtttactcgc tttgagttta
      781 tatgcccatt gcaatgcttc ttttttattt ccctggtttt tataacaatt caccaagaac
      841 catattgctg aactgtcatt attactggca gccaatttaa ttttttgtaa ccattcatta
      901 gcttccttgg tttcaccttg ttcttgtaac tctacatata accgatactg agctgtcgca
      961 tgaccattat tagccgctat ttttaaccac tgactacttt tttcttcatc ataattggac
     1021 aacttgtagt acaagaacaa cctaaaaccc gcctcgccat cgcctcgttt tgcctggtgt
     1081 gataaagtat ttacttcttc tggctgcaaa taaaagacgc ttactgttga ttttggttca
     1141 ccttcacctt caccttcacc ttttctagca gaaagctccg ccaataattt ttccgcctca
     1201 tcagaatgtt ttgagttatc catagcagcc ctcactccaa agttataaag acataccatg
     1261 ataatcaaca aaaaagtaaa tactttcatt actatttgct tcctgtaggt gtaaacttat
     1321 gaaagtcccc tggttgtttc tcgtctggca ccaattcaaa caccccgttg tccttaccgc
     1381 ctattgttat cctagctgtt tgccccataa tagcatagtt tccatccggc caaatccata
     1441 ttatttcatc aggagcaatc actgtagcat caagttcgtt agctagtttc tgtgcaaatg
     1501 aatttttgcc ttttcctgtt tcgcaggaaa acaagactac aggcatacct tcatgatatt
     1561 taggattcgc tcttatgtcg ctagctaatt gttttattgt cagatcactt ccttttttga
     1621 ttctgtcatc actcatagat gtaggcgaac catgacctcc aactgtaaaa cccgttggaa
     1681 attcccttac attaagagaa cctttatata taggatctga cggagaataa taattcaaat
     1741 caagtgctaa ccccaacgga tctatatgtt caataggatt gagtgggtac tgatacaggt
     1801 tccatccccc ctccagccct atcgggtcct gggtgatata tctcccctgc aacggatcat
     1861 agtaccggtt gcggttgtag tacagccccg actcctcatc atactgctgc cccggcagac
     1921 ggagtgactg ttgaaggtgc tgcgcgctgg tttcgcccag caggtttccc cactcgtcat
     1981 actccccctg ccacgccgtt tccccttccg ggctgatgag cgccagcggc agtccccggt
     2041 ggtcgcagtg gtaaagatga agtttcctct ccgggatgta ttccgcctcc agctgcgcgg
     2101 ccatctgctc cgccgtcagc ccgcactgcg caagccactg ctggctttct tcactcacgc
     2161 tgccctgccg cagctcccgc tccagcct
//
LOCUS       DAZFDC010000294         2153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFDC010000294 DAZFDC010000000
VERSION     DAZFDC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.296"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1439)
                     /locus_tag="ACHJ48_004980"
     CDS             complement(<1..1439)
                     /locus_tag="ACHJ48_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4710327.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSASVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSK"
     gene            complement(1414..1959)
                     /locus_tag="ACHJ48_004981"
     CDS             complement(1414..1959)
                     /locus_tag="ACHJ48_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4710328.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
BASE COUNT          499 a          617 c          513 g          524 t
ORIGIN      
        1 tttttcgagc gttcatacac aatggtcggg tcaatcccgc cagtatccca gcagatacgg
       61 gataccgaca tttctgcacc attccggcgg gtataggttt tattgatggc ctcatccaca
      121 cgcagcagcg tctgttcatc gtcgtggcgg cccataataa tctgccggtc aatcagccag
      181 ctttcctcac ccggccccca tccccatacg cgcatttcgt agcggtccag ctgggagtcg
      241 ataccggcgg tcaggtaagc cacacgatca ggaacggacg ctgaataatg ctctttccgc
      301 tctgccatca cttcagcatc cggacgttcg ccgattttcg cttcccacgt ctcaccgagc
      361 gtggtgttca cgaaggtttt acgttttccc gtatcccctt tcgtcttcat ccagtctttg
      421 acaatctgca cccaggtggt gaacgggctg tacgccgtcc agatgtgaaa ggtcacactg
      481 tcaggcggtt caatctcttc accggatgac gaaaaccaga gaatgccatc acgggtccag
      541 atcccggtct tttcgcagat ataacgggca tcagtaaagt ccagctcctg ctggcggatg
      601 acgcaggcat tatgctcgca gagataaaac acgctggagg ggtcatccgg cgtccatttg
      661 aggccaaacg gcgtctcttt gtcgccaaat ttaagatact gctcctcccc gcagtgcggg
      721 caggcaacat gaaaacgcat aaaatgcggg gattcactgg ctgcacgctc aatctggcag
      781 gtgcctctca ctttgggcgt ggagccacgg atggactttg gccagaccga gccttcaata
      841 cgcttgtcac ccaggaacgt cggagagcct tcctgttcaa tatcctcatc aaaggcagca
      901 agttcatcat aacccgccac atcaaccgac ttttcacggt agttttttgc cgctttaccg
      961 cccaggcacc agaagccacg accattggtg aaacgcttca tagtgagcgt gttatcccgg
     1021 tgctttttgc cataccacgg ggccagagac agcagcgacg gaatatcgcg gatggtaggc
     1081 tcgacgtggg ttttcataaa gttctcggca tcaccatccg tcggcaacca gataagggtg
     1141 ttacgctgct tatgctctat gaagtaggca taaacaccca gcagcatttt tgaataacca
     1201 acacgggcag acttcaccac attcacctcg cggatgtagt cgctgcccat cgcattcatg
     1261 atagcccgct gaaagggtag tgtttcccag cgcccttcct ggtatgcgga ttctttcggg
     1321 agatagtaac tggcatccgc ccattcaacg gcagtctgtg gctccggcct gaacagggct
     1381 cgcagcccgg cgcgtacatc acgccgcaga atatcaatct gactgttcga tatattcact
     1441 cagcaacccc ggtatcagtt catccagcgc ggctgctttg ttcatggctt tgataatatc
     1501 ccgtttcagg aaatcaacat gtcggttttc cagttccgga aaacgccgct gtactgagag
     1561 agggatcccg tcaagaatac tggcaatttc acctgcgatc cgtgacagca cgaaagtaca
     1621 gaatgcggtt tccaccactt ctgcggagtc tctggcattc ttcagctcct gggcgtcagc
     1681 ctgcgcacgc gtaagtcgat ggcgttcgta ctcaatagtc ccaggctgga gatctgcctc
     1741 gctggcagcc ctgtaatcct caacctcttt acggagtttt tcattttcga tatcagcttc
     1801 cctctgcgca taccactgaa ttgccgtggc ggtatcaaat acagattcaa cgcccttacc
     1861 acctccggag acgcaaggga gcccctgaga ctgccagcgt tcaatcgttc gcggatccac
     1921 gttgaaaatt tcggcaagtt tctttttatt aaccttcatg aaacagtctc acaacaaata
     1981 cagggtccga catgaaagtg cccgaaaatg actttttcag gcgttttcat gtcggacctt
     2041 tacggattcg atattagaaa aaacaaatag ttatgttcga gaagtaccga catgattttc
     2101 cccggaaaat tttcataaat agcgaaaacc cgcgaggtcg ccgccccgta acc
//
LOCUS       DAZFDC010000295         2093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFDC010000295 DAZFDC010000000
VERSION     DAZFDC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.297"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1329
                     /locus_tag="ACHJ48_004982"
     CDS             <1..1329
                     /locus_tag="ACHJ48_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO4710329.1"
                     /translation="TVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFID
                     TVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDI
                     AIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGAIVAHILSS
                     HYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIA
                     FCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDP
                     LTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNI
                     IGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKV
                     TISIGAVVTQGNALNPNEIYRLVDNALYEAKETGRNKVVVRDVVNFCESP"
     gene            1427..>2093
                     /locus_tag="ACHJ48_004983"
     CDS             1427..>2093
                     /locus_tag="ACHJ48_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HFO4710330.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGV"
BASE COUNT          607 a          344 c          452 g          690 t
ORIGIN      
        1 actgtattag tgagcttatt gtttgggctt gctttggttt tggtgaatag ttggtttaat
       61 cagccaggcg ttgaggaagt tgttccacga tcaacctatc tgatggtaat gattgcttta
      121 ttttttatcg atactgttgc atttattttt atgcagttgt atttcattta tgaccgtagg
      181 caattttcaa actgtatact tagtttggct tttctgagtt gtttgattta ctttgttaaa
      241 accgtcatta tcattcagca aattattgag gggcgtttga caagcagtgt tgtccaaaat
      301 gacattgcaa tctattattt gtttcgtcag atgagtttgt gcatattaat atttctggca
      361 ttggtgaata aagttagtga aaacacaaaa cagcgcaatt tattttccaa aaaaatgact
      421 ttgtgcataa gtttgttttt tgtttttggg ggggcgatag ttgctcacat actttctagt
      481 cattatgaga gctacaattt acacattgcc gagttaacca atgaaaatgg tcaggtggtt
      541 tggaaagcct catatgttac cataatgatt ttcatgtggt taaccttgct ttcggttaat
      601 ctatacttta atggtttacg ctatgatatc tggaatggag tgacggtgat tgctttttgt
      661 gccgtactgt acaatatctc tctgttattt atgagtaggt atagcgtgtc cacttggtat
      721 attagtcgca ctattgaagt agtcagtaag ttaaccgtta tggtaatatt tatgtgccat
      781 atattcagtg cgctacgagt aacaaagaac attgcacatc gcgatccctt aaccaatata
      841 tttaacagaa attatttttt taatgaactg acagttcaat cagcatcagc caaaaaaacg
      901 ccttattgcg tcatgattat ggatatcgac cacttcaaaa aagtcaacga cacctggggg
      961 catccggttg gcgatcaggt gataaaaaca gtggtgaata tcattgggaa aagcatacga
     1021 ccagatgacc ttttagcgcg cgtcggcggc gaagagtttg gcgtcttgct gacggacatc
     1081 gatactgaac gtgcgaaagc tttagcggaa aggattcggg aaaatgttga gcgtttaact
     1141 ggcgataatc ctgaatacgc tataccacaa aaagtgacga ttagtattgg cgctgttgtg
     1201 actcagggaa atgcgttaaa cccgaatgag atctatcgac tggttgataa cgcactatac
     1261 gaggcgaaag agaccgggcg taataaggtg gttgtgaggg atgtggtgaa tttttgtgag
     1321 tcaccataaa gcggcatttt agcgggtgat tttggtaagc gctaaatagc tattcgcaga
     1381 catgcgtaac aattttcaga aatatcatag tactatatga tcttaaatgc ttatcattga
     1441 tcagtctgct atcacccgct tttactggca attgtactat aatttcttag gttgcatcac
     1501 cctgatgcgt ggggaaacat gtatggtatt caacaaaaaa atgtttgtac tcattataat
     1561 accaggcatt ctgggggtgt tactgtcgtt tgctatgtca gtatttcaaa tgaatcgaga
     1621 tacgacaatt accgccggta ttctgttaaa acagcttgat aatgttacgc aaattgtcaa
     1681 acatacgaca aagcttactt ctattctggt gatgaaaccc tgcaaagata tcctggaaca
     1741 attgattgct aacggagcat tgacccccta tgttcgaaca acgggtctta tagaaaataa
     1801 tttccaaata tgttcttcag ttagcggttt caaaaaaatg aatgttaacg atgtttacgg
     1861 tacgagcttt cacaataaaa ataaagaatc acgcatagta tcaattagcg gtacaagttt
     1921 tgtaccaggt aaaacagcca tagtgttcct tatgccaata gggaatgata tgacagcttt
     1981 tagtattgta gagtcacgat atatctatga tctgatggat gttcttgatg atgagaatga
     2041 tgattcattt tctcttcgct ttactgaagg gcctgcaatt attagtggtg taa
//
LOCUS       DAZFDC010000296         2081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFDC010000296 DAZFDC010000000
VERSION     DAZFDC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.298"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            147..530
                     /gene="cesF"
                     /locus_tag="ACHJ48_004984"
     CDS             147..530
                     /gene="cesF"
                     /locus_tag="ACHJ48_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesF"
                     /protein_id="HFO4710331.1"
                     /translation="MNEKFRTDLAHTFGIALEEQTDVLSFHDNDGHEWILECASQSEI
                     LFFYCYLLNSESIQINSILEMNSNRELLGMFFLSLKDDNILLNIAFPADKIDITEFAN
                     LMENGYLLKNEIIRSLSSRPTDFLP"
     gene            complement(728..1234)
                     /gene="espH"
                     /locus_tag="ACHJ48_004985"
     CDS             complement(728..1234)
                     /gene="espH"
                     /locus_tag="ACHJ48_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspH"
                     /protein_id="HFO4710332.1"
                     /translation="MSLSGAVFKTFLTSEHASWNRFNRRLHIPNEDIVDEIQLKARMQ
                     QRHHRVYPEIGDSTIVSFRGKDYAVHFIKDGPKDDYVYKVQRITPENGCFSTLFSVFS
                     GGVTKALERKLNERHITPLSSTWFPRTPLEGILAERGLSSLLRRVQSTERLDNRAIAT
                     RASSYSVL"
     gene            complement(1265..>2081)
                     /gene="escQ"
                     /locus_tag="ACHJ48_004986"
     CDS             complement(1265..>2081)
                     /gene="escQ"
                     /locus_tag="ACHJ48_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFO4710333.1"
                     /translation="HACHAYFREVSGHGIRFTLTYSGYEGRFWISEEQFIQWCQELFP
                     YSESRLIPEDTIKLMILWVMQTALPEGDVSVDDVQFTMLNKDIYPVIENNNGENRLNV
                     IILETTVQSLQYLINDNWQLVPHSNALFFDGYIVPGWTDYPVTELRVGDSLRLYHVDD
                     SQERRCWIVINTPLATVNLSDNNLSVTDVLAADLLSALSNETVMNRIYCAIGTVHIDI
                     HMLRNVKKDDIINSDGYHLFGGCQLIRNNTTIAYGSIVKINEDFYFTVSIVCD"
BASE COUNT          655 a          424 c          349 g          653 t
ORIGIN      
        1 gtagttgcac tacatttatc atatgctatc atgaatatgc aacactattc ccgtaatctt
       61 atatctacgc cttattattt gtatcagaac tctcggatta attacgtttg agtatatcaa
      121 catgaaaaga atggcaggac tacaatatga atgagaaatt tcgcacagac cttgcacata
      181 cgtttgggat agctcttgaa gagcaaactg acgtgctctc tttccacgat aacgatggcc
      241 atgaatggat actggagtgt gcatctcaat ctgaaattct gtttttttat tgttatttat
      301 tgaatagcga atcaatacag attaattcaa tacttgaaat gaatagcaat cgagaactgc
      361 tcggtatgtt tttcctgtca ttaaaagatg acaacattct tttaaatatt gcatttcctg
      421 cagataaaat tgatattaca gagtttgcta atttaatgga aaatggttat ctattaaaga
      481 atgagataat tagatccttg tcttccagac ctacagattt tttaccttga taatttctgc
      541 gctggatggg atcatccatt ccgtttaaca tactcttcat cacagggacc tctgatttct
      601 ggccgctaac gtctctgaag attcagcctg tattatctaa aagcaatgta atatcagtta
      661 aaaagcggaa aaactccaga attataacta ttctggagtt aagtcaggaa gaaaactcaa
      721 atgctaatca taatacgcta taagaggaag ctcttgttgc tatagcccta ttatcaagac
      781 gttcggtaga ctgtacacga cgtaacaaag aagataatcc tctttcagct aagatcccct
      841 ccaggggagt acgaggaaac caggttgaag aaagtggagt tatatgtctc tcatttaatt
      901 ttctttccaa agccttagtc acaccgccag aaaacacgct aaatagcgtt gagaaacatc
      961 cattttccgg tgttattctt tgtactttgt acacataatc atcttttggg ccatctttaa
     1021 taaagtgaac agcatagtct ttgcctctaa aagagacaat ggtggaatcc cctatttctg
     1081 gatatacccg atgatggcgt tgttgcattc gggctttaag ctgaatttca tctacgatat
     1141 cttcattggg tatatgaagc cttctgttga acctgttcca tgacgcatgt tcacttgtca
     1201 gaaatgtctt gaataccgct cctgataacg acatgataca tctccctata taacgtattc
     1261 ctgattaatc acatactatg ctaacagtaa aataaaaatc ttcgttgatt tttacaatac
     1321 tcccgtatgc tatcgtcgta ttattgcgta ttaactgaca accaccaaaa agatggtatc
     1381 catctgaatt aataatgtca tcttttttaa cattacgcaa catgtgtata tcaatatgga
     1441 ctgtaccgat cgcacaataa atgcgattca tgacagtctc attgctaaga gcagataaca
     1501 gatccgcagc cagaacatcc gtgacagata aattattatc actcagattt actgttgcta
     1561 aaggggtatt aataacaatc cagcaccttc tctcttgact atcatcaaca tgatagagtc
     1621 tcagactatc accaactctc aactctgtaa ctggataatc agtccaccca ggtacgatat
     1681 agccatcaaa aaaaagagca ttagaatgtg gaactaattg ccaattatca ttaatcagat
     1741 attgtaaact ttgtaccgtt gtttcgagta taattacatt aagtctgttt tctccattat
     1801 tattttctat taccggataa atatctttat ttagcatcgt aaattgaaca tcatcaactg
     1861 acacatcacc ttccggtaag gcagtctgca ttacccataa gatcatgagt tttatcgtat
     1921 cttccggtat cagccggctt tcgctatacg ggaataactc ttggcaccac tgaatgaact
     1981 gttcttctga tatccagaaa cgcccctcat agcccgagta tgtcaacgtg aaacgaatac
     2041 catgaccgct gacctcacga aaatatgcat ggcaagcatg a
//
LOCUS       DAZFDC010000297         2070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFDC010000297 DAZFDC010000000
VERSION     DAZFDC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.299"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..240)
                     /locus_tag="ACHJ48_004987"
     CDS             complement(31..240)
                     /locus_tag="ACHJ48_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO4710334.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(336..710)
                     /locus_tag="ACHJ48_004988"
     CDS             complement(336..710)
                     /locus_tag="ACHJ48_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4710335.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(725..1441)
                     /locus_tag="ACHJ48_004989"
     CDS             complement(725..1441)
                     /locus_tag="ACHJ48_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4710336.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(1507..1851)
                     /locus_tag="ACHJ48_004990"
     CDS             complement(1507..1851)
                     /locus_tag="ACHJ48_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO4710337.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(1848..>2070)
                     /locus_tag="ACHJ48_004991"
     CDS             complement(1848..>2070)
                     /locus_tag="ACHJ48_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO4710338.1"
                     /translation="VHIRGVNPDTGNSDNTMKADNPRNAFYWRFVEMGTVNMPPHPFV
                     RPAFDVRSEQAAQVAIARMNRAIDEVLRR"
BASE COUNT          456 a          674 c          499 g          441 t
ORIGIN      
        1 taaaaaaacc gcctcagcgg ttctcatcat tcatgactgt gctgcaaggc tcagcgcgtc
       61 ttccagcgcc gcaaacggat ccacctccgg cttatcctca tcctcgcccc agcagagcat
      121 ggcgtccttc agtgcaacat tcatcccctg tgccccgaaa accgctttca cgatctgtgc
      181 attacggata tccccgcgct catcacccag cggggatatc ctgtcgaact ccatccacat
      241 catcgcctcg ctcgcactca ggctgtggcg cagttcggat aaggtgcgcc ccagacggag
      301 cgcaagtcgc atcagaaagc gaatttccgg gcgggctact tttttctggc cgactctgca
      361 tcagcgatca gttccagtgc ctgacgcagc aaccgggcat gtaccggacc atagacggcc
      421 agcacctgct cacggtcgtc cggagtgaac acccgctgca ggtccgtatc acacaggaca
      481 tcgcagaaca gcgtcacatc cgcttccagg ttacggcggg ttttcgccac caccgacagg
      541 gtatcgtcat cctctccatc accattgagc acttcctgcc acagatacca ggcctctgcc
      601 gaaggctccc gcagcaccac gctgacatta ccccattccg gcaccttcac cgttttatga
      661 cgaaaccctg acagtctggc cagcgccagc gttttcagat cctttttcat gatgacccat
      721 ccccttatcc ggcggctgcg ctcactgtca cggtgcattc aacagacgtc acactctgtg
      781 ctttctctgc cgaatcggtc accacacagg tatatttccc cgcatcagcg gactgcgcac
      841 ctggcttact gaaggtgtct gtcgtctgcc cgtcaaccgg ctgaccatcc ttcttccagg
      901 cgtatttata cggcggcgtt cccccgttgg cactgactga cattgtcagc agcgcaccgg
      961 tattcacggt aagtgtctta tccagatttt tcacaaacgc cagcggtacc acaaaggaca
     1021 ccggtttgcc tttcagacgc agtgagaacg ttgcagccac cacgccgttg gtaccggatg
     1081 accaggtgtg ctggcgcact tccgccagga atttaaagcc cttaccggac ggaaacagca
     1141 ccttaaacgc atacaacgcg tcattgtcat aggcatcacg cagggcgttc tgggcctgat
     1201 tcagataaaa attgcccgac atggaaatct cagacgacgc ccccagacca ttgatgttct
     1261 cctgctctgt ggagcagagc gtggtcacat caatatcctg tttctgcccg gcggtgaact
     1321 gcacttcctt gatggtgcag tccaggcgca gatattccgc cttatccata gtttcagcag
     1381 tcgccggggc agatgaaatc atcacctgcg tcagctgtga gcgttcatac aaagcagaca
     1441 ttctgcctcc tgataataaa aaacccgcac gcggcggggt atgggttttg tagaaaaaag
     1501 aaaaagtcac accgtgacct gaaactccag ggttgcacgg taacagcggt tttccggaat
     1561 atagtcctgc atttcactga cggatcccgg ggccagcagc attatggctt cacgggcgtc
     1621 ctgacgtatc tgacgcgcct gcgtcacagt cccggcataa acgtctatct gcaccgacac
     1681 tgaggactcc gcctgcccgc ccatcacgtc cgctgacacc gatgaaatca ggctgaaaac
     1741 cacccacgga agcgccaccg acggcctgcc atccagcagg gggaccacat acgggtacac
     1801 ctgcccgccg gcaagatgcg ccagatgagg atacaaatcc gcctccgtca tcgtctcagt
     1861 acctcatcaa tggcccggtt catccgcgca atcgccacct gtgccgcctg ttcactgcgc
     1921 acatcaaatg ccgggcgcac aaacgggtgc ggtggcatat tcacggtccc catttccaca
     1981 aaccgccagt agaaagcgtt gcgcgggtta tccgccttca tggtgttatc gctgttaccg
     2041 gtgtccggat taacaccccg gatatgcaca
//
LOCUS       DAZFDC010000298         2066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFDC010000298 DAZFDC010000000
VERSION     DAZFDC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.300"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1663)
                     /locus_tag="ACHJ48_004992"
     CDS             complement(<1..1663)
                     /locus_tag="ACHJ48_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4710339.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEEKIAAQAQTFEEKLASQSVVLQKCVT
                     GDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVANIPV"
     gene            complement(1609..1830)
                     /locus_tag="ACHJ48_004993"
     CDS             complement(1609..1830)
                     /locus_tag="ACHJ48_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710340.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1875..>2066)
                     /locus_tag="ACHJ48_004994"
     CDS             complement(1875..>2066)
                     /locus_tag="ACHJ48_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710341.1"
                     /translation="EASLEMQSEPGGDSTTPSPVELVSMFQTGSVAIRAERWINWRRR
                     RTAAVAVITGVNYGSASGG"
BASE COUNT          411 a          672 c          503 g          480 t
ORIGIN      
        1 gtaccggaat attcgcaacc gcatcattca ccgccttctg cagaacatcc ggatcgtagt
       61 cacgaccgtc gcgcggaaca ggaatatggc tcactgcctc tttcaccatc tgctcaagca
      121 tcggacgcac atcatcaccc gtcacgcact tctgtaatac cacagactga gaagccagtt
      181 tttcttcaaa cgtttgtgcc tgcgcggcta ttttctcctc aaatgtgcgc tgtaaatccg
      241 ccagcactgt ggaaaattct tctcccagtg cacgaataat ggacagttcc cgttccgtca
      301 ttttctcagt atcccccctg aacatcgctt tcaccgcatc atgctctgtt tcactgattg
      361 ccttattacc gtcagatgcg ccgtcaggca gctgtgatga aactgttttc ccggcagacg
      421 cgaacggatc ctcacgggca tcacgacggg acagcgcctc cagactgtag ttctgctgct
      481 gaagatacag tgcatcaccg ccggccaggg gcggcaggtt ctcccgttta cgggcctcat
      541 tgggcgtgag aagcgtattt ttcaccgcat cccccagcgt tttcatgcgc cgctcactgt
      601 ccattctcag cagcgtggtg acatcaaatt ctgtactctc gttttccccc gtttccagcg
      661 cctcatccag taacagttca atggactcaa tcagcgtctg caggcactgg gaataatact
      721 gctgctccag cgcctccacg ttgtcactgg aaggcggttg tcccacgcca atcttgtagg
      781 ccgggacacg gaacaccgaa cagacaattt cagcggtcat cttcagttgt tccaccgtct
      841 gcgcatccac cggtgaaaac gtcgtggggt tgtattttgc cccgttgctc agaatggccg
      901 ttttccccgc attttcgcct gtatacccgc tgtcccagtt gctcttcagt tttttcgcat
      961 tttcttccgt aatactgccg gggatctcaa tcaccccgga cggcctgccg ccatttctga
     1021 aaaaagacgt cgaattttcc tgaatatgat gcccctgcgt ggccgccagc ccggcggcat
     1081 acaccggcgg caatcctata agcggatgaa aaaaacagtt aaaccggtcg tggatcactt
     1141 cccgggcagg caccgtcacc gcctccgtga tcccgcagtt ccggtccggc gtgatgcggt
     1201 agaacacctc gccgtcatcc gccaccagag gttcaacccg gctccagtcc agaatacgca
     1261 gttctttgat ctgcccccgg gcattacgga ttttcagcac caccgtattg ccatgacgca
     1321 gtttggcgtt cagccacagt tcaaaaaact ggatgcggtt ctgctgggcg ttgggacgac
     1381 gacagaggcg ggcaatatcc ccccggcgcg tttccctgcg tatcccatgc gcatccgtct
     1441 gcataagacg cagccgcatt ttggcgatat cctgggatat cagcgaaata catgcaaaca
     1501 ccgcatgaaa ggagaggacg gcttcaggat cggctttcac gccctgctgc caggcgccgg
     1561 aaaagggctc agccaccgcc tgaaacaggc tggtccagcc cgcctctctt acgtcacgtc
     1621 ctgatttctg gttttttcgg gttcgccgta aaaggttcca cattcgccat gctccgcatc
     1681 acgtttcttt ttctgacctg ccggacgtcg caccgtgatg tactccgcct ttcccaggcg
     1741 aaccagcacc tccgcacacg gctgtgccac atcacggata tccccggccc gggcatcatg
     1801 cgtgccctgc agatatcgga tctttgccat aacctgttac gggaggcgca cgcctcccgt
     1861 cctccttatc agactcagcc gccggacgca ctgccgtagt tcactccggt gatcaccgcc
     1921 accgccgcag tacggcgacg acgccagttg atccagcgct ccgcacggat ggccacgctg
     1981 cctgtctgga acatggaaac cagctccacc ggggacggcg tggtactgtc gccgcccggc
     2041 tcagactgca tttccagtga tgcctc
//
LOCUS       DAZFDC010000299         2037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFDC010000299 DAZFDC010000000
VERSION     DAZFDC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.301"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..263)
                     /locus_tag="ACHJ48_004995"
     CDS             complement(<1..263)
                     /locus_tag="ACHJ48_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO4710342.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDP"
     gene            complement(512..1918)
                     /gene="gndA"
                     /locus_tag="ACHJ48_004996"
     CDS             complement(512..1918)
                     /gene="gndA"
                     /locus_tag="ACHJ48_004996"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO4710343.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQI
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          549 a          470 c          466 g          552 t
ORIGIN      
        1 ttaggatcat agtcggttgg agtggcgatg atgacataat cagcatcccg gtaggcttca
       61 tttttatcta atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc
      121 ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg taaaatatct
      181 aatgccacaa cctcatgatt ttgagcgatt agaagcccgt ttgacaagcc tacatagcca
      241 gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg tgaatcatga
      301 tgtttttaac atcctgataa gagctaaaag ttttaacgct acgttgttcg atggtcaacg
      361 cagcgaaaaa caattcagat tttctttata agaatagctg atatttatta agttaatatt
      421 aagcagtgat atttagtcta taagataatt ggcaaaaaaa agcccggtac aatatacgcc
      481 gggcctcaat tttattgttg attaaatcag attaatccag ccattcggta tggaacacac
      541 cttctttatc aatgcgctta taagtatgcg caccgaaata gtcacgctgt gcctggatca
      601 ggttcgcagg cagaacagcg gcgcggtagc tgtcataata ggcaaccgca gcggcgaagg
      661 tcggaaccgg gataccgttc tgtaccgcat aagcgacgac atcgcgcagc gcctgctggt
      721 agtcatcggc aatttgcttg aagtaaggag ccagcagcag gttagcgatc tgcggatttt
      781 cggcataagc atcggtgatt ttctgcagga actgcgcacg gatgatgcag ccagcacgga
      841 aaatcttcgc gatttcgccg tagttcagat cccagttgta ctcttcagac gccgcacgca
      901 gttgagagaa cccctgagcg taagaaacga ttttgcccag atacagtgca cggcgaactt
      961 tttcgatgaa ctcagccttg tcgccagctg gctgcgcttg tgggccagag agaactttag
     1021 acgcagcaac gcgctgatct ttcagagaag agatgtatcg tgcaaacaca gactcggtaa
     1081 tcagcgacag cggttcgccg agatccagtg cgctctggct ggtccatttg cccgtacctt
     1141 tgtttgccgc ttcatccagg atcacatcaa ccaggtagtt accgtcttca tcttttttag
     1201 tgaagatgtc tttggtaatg tcgatcaggt agctgctcag ttcaccgtta ttccactcgg
     1261 taaagatctg cgccagttct tcgttggtga ggttcagacc acctttaagc agagaatagg
     1321 cttcagcaat cagctgcata tcgccgtatt caataccgtt gtgaaccatc ttcacatagt
     1381 gacctgcgcc atcggcacca atataggtaa cgcatggctc accgtcttca gccactgcgg
     1441 cgattttggt caggatcggc gcaactagtt cataggcttc tttctgccca ccaggcataa
     1501 tggaaggacc ttttagtgcg ccctcctcac caccggaaac accggtaccg atgaagttaa
     1561 agccttctgc agaaagctca cggttacgac gaatggtgtc ctggaagaag gtattaccac
     1621 catcaatgat gatgtcacct ttatcgaggt atggcttaag ggaatcaata gcagcatccg
     1681 tgcctgcacc tgctttcacc attaacaaga tgcgacgagg cgtttccaga gattcaacaa
     1741 attctttcac cgtatagtaa ggaaccagtt tcttgcctgg attttcggca atcacttctt
     1801 ccgtcttttc acgggaacgg ttgaaaatag agacggtata accacggctt tcgatgttga
     1861 gcgcaaggtt gcgccccatc actgccatac cgactacgcc gatctgttgc tttgacattg
     1921 tttactcctg tcaggggtta ccgctagata tgcggcttga ataacgtata aaaatgtatc
     1981 agagtattat aaatttcatg ctgtatcagt aataactaag taaaggatag cttaaaa
//
LOCUS       DAZFDC010000300         2035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFDC010000300 DAZFDC010000000
VERSION     DAZFDC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.302"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            15..491
                     /locus_tag="ACHJ48_004997"
     CDS             15..491
                     /locus_tag="ACHJ48_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO4710344.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            488..1492
                     /locus_tag="ACHJ48_004998"
                     /pseudo
     CDS             488..1492
                     /locus_tag="ACHJ48_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1696..>2035
                     /locus_tag="ACHJ48_004999"
     CDS             1696..>2035
                     /locus_tag="ACHJ48_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HFO4710345.1"
                     /translation="MKPREKDYAVYDGFGLLLNVSKAGGKVWRFRYSHPITKKRQTYT
                     IGRFPEFSLAEAREVRDELRRMIARGVDPVTEKKNRKIEMSLKNLQTFEAVANAWFAF
                     KKGSALRKPTL"
BASE COUNT          493 a          455 c          588 g          499 t
ORIGIN      
        1 gtggagggat gtaaatggac ggcgagctga aaaatatgaa gttaaatatt aatcaactgg
       61 cagccctttc aggtctgcac cggcagactg ttgccgccag aatggcggat gttcctcttg
      121 caccaggcag taatgaaaag aaaaaactgt atctcctgac ggatttgata acttcgttgc
      181 tggaaaaacc accatcttcc gaagatgagg atatggatcc tcacgctcgt aaggcatggt
      241 atcagtccga gcgcgagcgt cttaaatttc agcatgaaac tgttcagctt gtgccagtca
      301 gtgatgtcag gcggtccttt tctgtcgtgg tgaaagcgat agttcaggta ctggaaacct
      361 ggcctgaccg gctggagagg gacagggggt ggactgcatc acaactgaat gaagtacaga
      421 ttgtggttga tgagatccgc gacacactgg aaaaggcagt cattgactgt tgtgatgagg
      481 ccgatatgtg aatcaggtga acgagagcca tagccgcgca tccgatatct ggcgcgaagt
      541 ggcctcgctg tttcgcccac ccagccggtt accagtagcg gaagccatca ggcgttatat
      601 gcgggttcca cggggagcca atacttccgg tccgtgggag tcatcgctga cgccctatat
      661 gattgacccc attaatacat tatcagcccg tgaatatgac gcggtggtgt ttgtgggacc
      721 tgcgcgaacc gggaaaaccg aagggctgat tgatggctgg attgtgtacg gcatcatctg
      781 tgatccggcg gatatgctgg tggtgcagat gactgagacg aaggcgcgtg agcattccag
      841 aacgcgtctt tccaggacgt ttcgccacag tccggaggtc agcaagcgcc tcagtccttc
      901 ccgtaatgac aacaacgtcc acgataaaat gtttcttgac ggctccttcc tgaagattgg
      961 ctggccgtcg atcaccgtct tttcctcttc ggattaccgt cgtgtggcgc tgacggatta
     1021 tgaccgtttc cctgaaaatg tggacgggga aggggatgcc ttcacgctgg cctcaaagcg
     1081 taccaccacc tttatgtcct cggggatgac cctggtcgag agttcaccgg ggcgggatat
     1141 caccgatacc aaatggcgtt gtggtggcgc acatgaggca ccgccaacaa cggggatcct
     1201 gtcactgtat aaccggggag accgccgccg gtggtactgg ccgtgtccgc actgcgggga
     1261 atattttcag ccggtgatgg ataacatgac cggataccgg aataacccgg attttgtggc
     1321 tgccggtcag gctgcccgtc tgatgtgtcc gcattgtcgc gggctgattg cccctgagca
     1381 gaaacgcgaa ctgaataacc aggggatctg gcttcgtgaa ggtgaacggg cggcggcgga
     1441 cggcagtatc accggaacgc cacgaaactc ccggattgcg tcattttgga tgtagggtac
     1501 atttcttacc tgtttgtatg ttctggtgtc gattcatagt cttttcaatg agttgtgatt
     1561 ttatgagttt cctctctttg cttgataatg agttagttta tcgcttgtta ttggcttgaa
     1621 tggactacat gacggactaa aaaatgaggg cgatagatgt cggtaaagcc attaaccgtg
     1681 actgaagtta aggggatgaa accacgtgaa aaggactatg ccgtttatga tgggttcggt
     1741 ttattgctga atgtgagtaa agccggtggg aaagtgtggc gtttccgtta tagccatccg
     1801 ataacgaaga aacggcagac atacacgata ggacgttttc ctgaattctc actcgcggaa
     1861 gcacgggaag tacgtgatga acttcggcga atgattgcac gtggagttga tccagtgacg
     1921 gagaagaaaa atcgtaaaat tgagatgtca ctaaaaaatc tacagacatt tgaagctgtt
     1981 gctaatgcat ggttcgcttt taaaaaggga tctgcattgc ggaaacctac gctgt
//
LOCUS       DAZFDC010000301         2020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFDC010000301 DAZFDC010000000
VERSION     DAZFDC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.303"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..642)
                     /locus_tag="ACHJ48_005000"
     CDS             complement(<1..642)
                     /locus_tag="ACHJ48_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4710346.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQN"
     gene            825..1415
                     /gene="espM2"
                     /locus_tag="ACHJ48_005001"
     CDS             825..1415
                     /gene="espM2"
                     /locus_tag="ACHJ48_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO4710347.1"
                     /translation="MPMNTTGMSFSSFGISCHRENSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            1649..1801
                     /locus_tag="ACHJ48_005002"
                     /pseudo
     CDS             1649..1801
                     /locus_tag="ACHJ48_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1917..>2020)
                     /locus_tag="ACHJ48_005003"
     CDS             complement(1917..>2020)
                     /locus_tag="ACHJ48_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO4710348.1"
                     /translation="LSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
BASE COUNT          628 a          374 c          443 g          575 t
ORIGIN      
        1 gttttgttga agtgggtata tgggtaatgc gtaaaaacat tgttttgtta tattaaaatg
       61 acacttgtct ttgcttataa tcatttctgg agcaaagggg tcacggctga gggggtggag
      121 agcgttacgc aggataagtt cagtcaatgc ttcctgatca tataatgcac atatttctga
      181 atttcgagca tttctgacaa aaacacctgt ctcaggaata tgtagcgtta tcggacaatt
      241 aagatgttcc tcagtacagg aaaagtcttg taaactaacg ggaaatgcat tcgaatttat
      301 tcgatctagt agtgttttta tgacgctctc ctgcacaagc ggggcagctt gcctctgctc
      361 cagatataag ctgtatgcct gcagaaaagt gttacctccg ttaagttgtc ttgtcaggcg
      421 gtctgcgaga tgatgagcct ctcttgcccc aagatggtcc caaagtccac caagaatagg
      481 ctcaacacta aaaccatcca taaactggat ataatgaagc ctcatattac ggcctcctat
      541 agtaatagtg tcattctggt tgcttcttgc tatatgtcgt aaagcctcca gctcattttc
      601 cggcaataca cttccattag aaaaatttaa tatgactggc atattattat cctttttaat
      661 ccatattgtg aactatattg gtaacaagaa tcaacatgcc gctcatgata tgtgaataaa
      721 acaatacaat ttttaataga atattattgc atgcatgttt tttctggcta caataaacgc
      781 aagtatgcaa tattgtaaac acttttttat aaaaaggaat tataatgccg atgaatacta
      841 caggtatgtc tttcagctct tttggtataa gttgccatag agagaactcc ttcaggaact
      901 ctttccgagg gaagaatgat gaggtcgtaa aatgttcaat tggagaacgg actattagct
      961 tttctgttcg caaatttagc ggcaatatac tggaaacggt gagaaggcag agtactaaag
     1021 atattgatga gtggataaaa gatgaacgga tagtatatcc ctcaagggtg atcaaccagg
     1081 agattgataa ttactgtttt caaaaaaatg caaaaatttc cactgaagag aggcaaagag
     1141 tcttttttct tgtgagccag gagaatcaac taacccttga tgttaaggca gcacaaagtt
     1201 ctattaatca tgtaataatg gggagtgctt cttttggcaa gaaaatggat gctctttgtg
     1261 atggtatgag ccgggatgta aaaaatcgta catcagatac catagcaaac ttgcttgcgg
     1321 acaagtttta ccagaaacat atcgattcgg atattgatat tgtaaaacta cgaaatgata
     1381 tcccagatta tttgatgcgt gctatacagg gatgaggcta gaggagagga ctgcttcagc
     1441 atcatcggct tcggaccagt aaaagagacg cagaaaatgc acaacggcac cacacgccat
     1501 gcatggattc agattactca tggctcacca gtacagctat aaatcgtaaa gaaaactaca
     1561 gtacgttata cacagaaacg taccgtgatt cattatttat tgtacaggtt catcgcagcg
     1621 acaatatttt gtgatgtttg cgtggtgcgc cccacttcag gatgctgcag atctggaaat
     1681 tgcaacggag gaagaaacct cattgctgga agcctagaaa aagtatcggg tgttgctgaa
     1741 ccgtgttgat acatcaactg cacctgatat tgagtggccg gaagaaccag acacaatgta
     1801 agcgaaaaag aaaaaccgca gacacgacgt atgcaggacg tgctgcggtt ggctggtgaa
     1861 ctttcgatag tgcgagtatt gaatgatttc cagccgttac cgattttacg tgttaattag
     1921 tgaacaaacc actcgtcagc agacttccag gtatctttca gagtctcctg aacaaaagtt
     1981 ttagctgaat ctttatcggc ggtgcgcgta acagaaaggc
//
LOCUS       DAZFDC010000302         2002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFDC010000302 DAZFDC010000000
VERSION     DAZFDC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.304"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..310
                     /locus_tag="ACHJ48_005004"
     CDS             <1..310
                     /locus_tag="ACHJ48_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710349.1"
                     /translation="NYTCKIKDAADEIKGSMIFNDVDGKQKATCTIRVLILKTFHDGR
                     YRFVIESVIYDLINNYSGFILTGRLFWQGEGFGHELFPVTNKYNAWRWKNKKIIDISW
                     "
     gene            479..1813
                     /locus_tag="ACHJ48_005005"
     CDS             479..1813
                     /locus_tag="ACHJ48_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HFO4710350.1"
                     /translation="MTASGTGLGYGEGDESYGYDSCGYLKAQSAGGHRISEETDQYAG
                     GHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEY
                     RHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDELYSVRHLVFNGFELISQQC
                     SRVRQPHPSVAPQWVTRTNHAVNDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPAD
                     TDYPDPRGERDAEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGE
                     QTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTGFLDGVRLSRSQINNIA
                     KEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLYEVYHEVIHAKQFAKIG
                     REAYEALGRLSREEHVLNEILKSKNLFNEAEIAHAIKYVEGLREKFMMGLIN"
     gene            1810..>2002
                     /locus_tag="ACHJ48_005006"
     CDS             1810..>2002
                     /locus_tag="ACHJ48_005006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710351.1"
                     /translation="MIKHNKITIEMALDLARRELELREIPYIKNSLHANYSYKSISIG
                     SKQGWLISAKLKVPETFEPD"
BASE COUNT          587 a          402 c          565 g          448 t
ORIGIN      
        1 aaattacaca tgcaaaataa aagatgctgc tgatgaaata aagggtagta tgatttttaa
       61 tgatgtagat ggtaaacaaa aagcaacttg cactatcagg gtgttaatac taaaaacatt
      121 tcacgatggt cgttatagat ttgtaattga atctgtgatt tatgatttaa ttaataatta
      181 cagtggattt attttaacgg ggagattgtt ttggcaaggg gaaggttttg gccatgaatt
      241 attccccgtg acaaacaaat ataatgcttg gagatggaaa aataaaaaaa taatagatat
      301 ttcttggtaa ggaaaatatt ccccgaaaga ggccacatgg ggaatactgc tcccctcact
      361 gatacgggag taccgttacg acagcgcggg taacgtatca ggcgtcacca gccgggaaga
      421 ttacggacgg gaaacacagc gggagtaccg gcttgaccgg aacggccagg tcacggcggt
      481 gacagcctca ggcaccgggc tgggctatgg cgaaggcgat gagtcctatg gctatgacag
      541 ctgtggctac ctgaaggcgc agtctgcggg cgggcaccgg ataagtgaag agactgacca
      601 gtatgccgga ggccaccggc tgaaacaggc cggaaacacg cagtatgact atgacgccgc
      661 aggccggatg gtcagccgca ccagacaccg tgacggctat cgcccggaaa cagagcggtt
      721 ccggtgggac agccgggacc agctgaccgg gtattgcagc gcacagggtg agcagtggga
      781 ataccgccac gacgccagcg gcagacgaac agagaaacgc tgcgaccgga agaaaatccg
      841 cttcacgtac ctgtgggacg gcgacagtat tgcggaaatc cgggaatacc gcgatgatga
      901 actgtacagc gtacggcacc tggtgtttaa cggctttgag ctgataagcc agcagtgcag
      961 ccgggtacgg cagccgcacc cgtccgtggc cccgcagtgg gtgacacgaa cgaatcatgc
     1021 ggtgaacgac ctgacgggcc gtccgctgat gctctttaac agtgaaggta aaaccgtctg
     1081 gcggccggga cagaccagcc tgtgggggct ggcactcagc ctgcccgcag acacagacta
     1141 cccggacccg cgcggggagc gggacgcgga agccgacccc ggcctgctgt atgcaggaca
     1201 gtggcaggat gcagaatcgg ggttgtgcta taaccggttc cgatattacg agccggaaac
     1261 cgggatgtac ctggtgagtg atccactggg gttgcaggga ggggagcaga cttaccggta
     1321 tgtgccgaat ccttgtgggt gggttgatcc gctgggattg gctgcaagtt ctaaaatcag
     1381 cagtttgatg gactatattg gcgatggtcg tcgtgttagt gggcatacgg gtttcctgga
     1441 tggggttcgt ttatcacgta gtcaaataaa caatattgct aaagaaatgg agaagctagg
     1501 aattaaagta ataaggaaag cagataaata tttgccacca aatgctaggg cagcttttga
     1561 ttatggtctt cgcaatattt atcttaggaa aaatgctacc ttatatgagg tgtatcatga
     1621 agtgattcat gctaagcaat ttgcgaaaat tggacgagaa gcatacgaag cactaggacg
     1681 tttatctagg gaggaacacg ttctaaatga aatattaaaa agtaaaaatt tattcaatga
     1741 agcggaaata gctcatgcca taaaatatgt tgagggattg agagaaaaat tcatgatggg
     1801 actaataaat tgataaaaca taacaaaata actatcgaaa tggctcttga cttggctcgg
     1861 cgtgagttag agttaaggga aattccttat attaaaaata gtctacatgc caattacagc
     1921 tataaatcta ttagtattgg ttctaagcaa ggttggttaa tttcagcgaa attaaaagtc
     1981 cctgaaactt ttgagcctga ta
//
LOCUS       DAZFDC010000303         2002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFDC010000303 DAZFDC010000000
VERSION     DAZFDC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.305"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..321
                     /locus_tag="ACHJ48_005007"
     CDS             <1..321
                     /locus_tag="ACHJ48_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4710352.1"
                     /translation="TRRVNVLKGEQRRVSGSARVSLKRRIWLRDAGQCCLCGRVVDLC
                     DSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPDPTLPEVSGGSGRADD
                     IIGL"
     gene            610..1173
                     /locus_tag="ACHJ48_005008"
     CDS             610..1173
                     /locus_tag="ACHJ48_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4710353.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            1170..>2002
                     /locus_tag="ACHJ48_005009"
     CDS             1170..>2002
                     /locus_tag="ACHJ48_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4710354.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     V"
BASE COUNT          462 a          502 c          617 g          421 t
ORIGIN      
        1 acccggcggg tgaatgtcct gaagggtgaa cagcgtcggg tcagtggcag tgcccgggtt
       61 tccctcaagc gtcgtatctg gctgagggat gccgggcagt gctgtctctg tggtcgtgtg
      121 gttgacctct gtgacagtga actcgatcac cgaattgcac ttcagttcgg tggtggtaat
      181 gaggagacga atctctggac gctctgtacc gaatgccatc gacaaaagtc tgctcgtgaa
      241 gcggcgggtg gtatgccgga cccgacgctg ccggaggtgt ccggaggtag tggcagagcg
      301 gacgacatca tcggactgta acccgaccgg gggggtatca ttcggcgtaa aaaacgatcg
      361 ctccggacac cgccccccct ctcacgcaga gaaaaaattc ccgtttcagg gcagttaaca
      421 tgttaactgg ctgtccgggc atttttgcgg tttttatctt tattattcag tttgttgtgc
      481 gaaaaaaatg ttaactggct ttttcagcaa atgttaacca ggcagcagtt aacatttgcg
      541 gcatgagacg ccgggaaaaa tgggctgaac catacccggc tgagtgcgtt ctggacccgg
      601 gaggaggctg tgctgacaac gcaaaaacga aaatttgcgc tggcgctcat gtccgggaaa
      661 aacaaaacag cgtcagccat tgccgctggt tattcggcga agaccgccag ggttaaaggc
      721 tcgcagctgg caaaagatcc ggaggtgctt gcgtttatag cccgtaaaca atgcgagacg
      781 gtggaggtgg atgaggttcc tgtttaccgg cagaaaaaat cagagcagga ggataaaccc
      841 cgtcgccgtg aggcggctgc aataccacag ccggacgaaa acaatccgga gatgccaccg
      901 tccgcggtga tgtctcctgg tattgaatat atggaggatg gtcttcccga tccggtgaaa
      961 gcgatggggc gtcttctggt ggagaacatt aataccgacc ccaggctggc gctggatgcg
     1021 gcttataagc tggcgcagtt cacgcaccat aaaaaagggg atgccggtaa aaaatcggca
     1081 aaaggtgacg cggcgaaaaa agcggctaac cgttttgcgg tgccaccacc accccgcctg
     1141 gtggtgaata atgataatga gggcaacgga tgatacctgt gtggagcacg gcctgcccgg
     1201 actgggcaga gcgcctgaaa aaggggctgt cgattattcc ggctccgatt tatccggacc
     1261 aggctgcaca tgcactggcg atttttaaac aactgcgaat tgtggatgca ccgggtagcc
     1321 cgacattcgg ggagtcctgt gcaccgtggg tgtttgacct ggtggcggcc ctgtttggct
     1381 cctacgatgc gcagaccggt gttcgccata tcaaggaagt gtttatcctt atccccaaga
     1441 aaaactcgaa gtccacgctg gctgcgggga tcatgatgac ggcgctgtta ctgaactggc
     1501 ggcaggcggc gggctacacc attctggccc cgaccgtgga ggtggcggct aacgccttca
     1561 accctgccag ggatatggta cgacgggacg atgatctgga tgacctctgt caggtgcaga
     1621 cacatatccg gaccatcacc catcgggtga cggacaccac cctgaaggtg gtggcagccg
     1681 atccgaatac ggtgtccggt atcaagtccg tggggactct gattgatgaa ctgtggctgt
     1741 ttggcaagca gtacaaggcg gaagacatgc tacgtgaagc catcggcggg cttgcctccc
     1801 gtccggaagg gtttgtggtg tatacgacca cccagtcgaa tgagccgcca gccggggtgt
     1861 tcagacagaa actgcagtac gcccgggatg tccgtgacgg caaaattcat gatccgcatt
     1921 ttctgccggt gatatttgaa caccctcctg aaatggtgga aagcggggct cacctgctga
     1981 tggaaaacct cgccatggtc aa
//
LOCUS       DAZFDC010000304         1976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFDC010000304 DAZFDC010000000
VERSION     DAZFDC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.306"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1976
                     /locus_tag="ACHJ48_005010"
     CDS             <1..>1976
                     /locus_tag="ACHJ48_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO4710355.1"
                     /translation="QTQKIKDIVDFLGIQDNTIKVKLESDIKPISEIQQPLHSILSRQ
                     KEHVKNLLSGLLDEFSNKLRKQGLSLKTNVLSVNNFKESKINSDTVEVTVTDLQGRLY
                     RVDIDTRVIGLTFKEGINSLSEALEHMNIDAIMSVIGLVQYARMIKMNDNISAIDHAG
                     AVSDIKNIVDKFLGGILTLTNNRVYNPGGVSGASLEGFISSGLEVCASRMGGTAGRYL
                     SNVAKVIKLPLLDIGINIWSLYDSSLNHAKATTQIEYISTAIDVSFSSINTALSIGAI
                     AYPPLAIAIVPITIFSHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKATG
                     IIDLSNNQVLGNIYLDMRENPPILHGEKSYNSGKNIGSHPDMTDREIMESRAYNFACT
                     KLSDAGEPDIFGWGDKEICNSMELSESQLANGYSNRQWPSQIPVIPEGIYNTVYLGYG
                     ETLRANTEVTLSSTGYFYEIARAYTDDELSEPLLTVCNQHSHVIGGKEPLTIIIPAIE
                     HGMLGSNLHMIERFKNYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRD
                     FNLDLDLSDGRKQNFNFHHPSGFYSGKVMSITQKGINAVVGTKLGYDKIRGNNLDNTF
                     SLGSGGGVIYSGGGSNTYFVPATLQDNLHIYISE"
BASE COUNT          677 a          294 c          395 g          610 t
ORIGIN      
        1 gcaaactcag aagataaaag atattgtgga ttttttaggt attcaggata acacaataaa
       61 agttaagctt gaaagtgata ttaaacctat aagtgaaatc caacagcccc ttcattccat
      121 tctttcacgg cagaaagaac acgtaaagaa tttactatct ggattgcttg atgagttttc
      181 aaataagtta agaaagcaag gtctttcact taaaacaaat gttctttcag ttaataactt
      241 caaagaatca aagataaata gtgatacagt ggaggttaca gtaacagatc tgcaaggaag
      301 actttatcgt gttgatatag atacaagagt tattggctta acttttaaag aggggataaa
      361 tagtttatcc gaagcactcg aacacatgaa tatcgatgcg attatgtctg ttattggctt
      421 ggttcaatat gcacgaatga taaaaatgaa tgacaatatt agtgcaatag atcatgcagg
      481 agcagtatca gatataaaaa atattgtaga caaattttta ggcggaattt tgactcttac
      541 taataatcgc gtatataatc ctggcggtgt atctggtgcc tcgcttgaag gatttatatc
      601 ttctgggttg gaggtgtgtg cttcaagaat gggtggcacc gcagggcgtt atcttagcaa
      661 tgtggcaaaa gtaattaaat tacctttgct agatataggc ataaatattt ggtcattata
      721 tgattcttca ttaaatcatg ctaaagccac cacacaaatt gaatatatat caacagcaat
      781 agatgtatct ttttcatcta taaatacagc tttgtcaata ggcgcaatcg catatcctcc
      841 tttggcaata gctatagttc cgataactat tttttctcat gaagtaaaaa actatgctgt
      901 ttatgtgaat caaattaatg agcgccataa gttatggctc gaagctgaaa agtatctgga
      961 caatggaagt gcaaaggttc taagcattaa caaagccaca ggcataatcg atttatcaaa
     1021 taatcaggtg ttaggtaata tttatttaga tatgagagaa aatcctccaa tattacacgg
     1081 tgagaaatca tataatagcg gaaagaatat tggtagtcat cctgatatga ctgacagaga
     1141 gattatggag tctagagcat acaattttgc atgcacaaaa ctctcagatg caggtgaacc
     1201 cgatattttc ggttggggag ataaggaaat atgtaattca atggaattat cagaatcaca
     1261 attagctaat gggtattcta accgccaatg gccttctcag attccagtta ttccagaagg
     1321 aatttataat acagtatatc ttggatatgg agaaacattg cgagctaata ctgaagttac
     1381 attgagtagc actggttact tttacgagat tgcaagggca tatactgatg atgaactatc
     1441 tgagccatta ttaacagtat gtaaccaaca tagtcatgtt attggtggaa aagaaccatt
     1501 aacaattatt attcccgcta ttgaacatgg tatgcttggc tctaatctac atatgattga
     1561 acgttttaaa aactataatt tctcgatatc aggagggaag ggagggataa aattaatggt
     1621 tggtggtatt ggtgattaca acatagaatg tacccccggt gttagaaata ttatatcgtt
     1681 tgaacaatta tcacgtgact tcaatctgga tttggattta tcagatggga ggaaacaaaa
     1741 ttttaatttt catcatccct caggttttta ttcaggaaaa gttatgagca taacgcaaaa
     1801 gggtataaat gcggttgttg gtactaaact gggatatgat aagattcgtg gtaataatct
     1861 tgataatacc ttttctcttg ggagtggagg aggtgttata tattctggag gagggagcaa
     1921 tacctatttc gttcccgcca cactacagga taatttacac atatatatat cagaaa
//
LOCUS       DAZFDC010000305         1976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFDC010000305 DAZFDC010000000
VERSION     DAZFDC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.307"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..96)
                     /locus_tag="ACHJ48_005011"
     CDS             complement(<1..96)
                     /locus_tag="ACHJ48_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="escC domain protein"
                     /protein_id="HFO4710356.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKSE"
     gene            complement(96..551)
                     /gene="sepD"
                     /locus_tag="ACHJ48_005012"
     CDS             complement(96..551)
                     /gene="sepD"
                     /locus_tag="ACHJ48_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO4710357.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSNEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQLSSSDTMMPPTHLFSDIGRIV"
     gene            complement(557..1129)
                     /gene="escJ"
                     /locus_tag="ACHJ48_005013"
     CDS             complement(557..1129)
                     /gene="escJ"
                     /locus_tag="ACHJ48_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO4710358.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(1132..1509)
                     /gene="sctI"
                     /locus_tag="ACHJ48_005014"
     CDS             complement(1132..1509)
                     /gene="sctI"
                     /locus_tag="ACHJ48_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO4710359.1"
                     /translation="MNNINQSENINIQLNKAPQTNFVDEHTSLASAPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNSDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(1593..1892)
                     /gene="espZ"
                     /locus_tag="ACHJ48_005015"
                     /gene_synonym="sepZ"
     CDS             complement(1593..1892)
                     /gene="espZ"
                     /locus_tag="ACHJ48_005015"
                     /gene_synonym="sepZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE cytoprotective
                     effector EspZ"
                     /protein_id="HFO4710360.1"
                     /translation="MEAANLSPSGAVMPLATSLSGNNSVDEKTGVIKPENGTNRTVRV
                     IAGLALTTTALAALGTGIAAACSETSSTEYLALGITSGVLGTLTAVGGALAMKYA"
BASE COUNT          542 a          389 c          382 g          663 t
ORIGIN      
        1 ttcgctcttg cctaatcttt tttccagaga ggacggggca gcttgtgcac tgcagcaaaa
       61 tagtgctgta aaaataaaaa aacttatttt tttcattaca caattcgtcc tatatcagaa
      121 aacaagtgtg ttggcggcat cattgtatcc gatgaagata attgctctag tttatcaaat
      181 tgcttatcga gcaaagaggc gagagtatgt tgcattattg cttttgacat gtttgcgcga
      241 tgattattat ctttatgaaa aatttggcaa agataccact tcttattaga caggaaaata
      301 cccatttgtt tatattcgtt actaacggcc aggatattgc agagtctgat aaatgtatct
      361 gttggaacct gacgaaaatc accactaagc tcaatatata gaacccagta gtttccaaaa
      421 tcgtgcaaga aagcattttt ttggtagata taaaaagtga gatgcgttgg tgaaccgttg
      481 acatccttta caaaatcaag ggccgttagc cagtcacaat cattctttgc tatgccatta
      541 ttattgttca tacatattac ccgtcctgtc ctgaggatga cttgataaca acactaatat
      601 tttctaattt gagatcgtca acactattct taactaggtt ttttatctga atgaccgatg
      661 gtgctaagtt aacctctggc gagctgatga ctaaaacggc tgctgaggag ggttgacttt
      721 cgttattgtt aacatttaat gaaacgctgc aatcgataac accaggtatt ttacttagca
      781 acctttcgat gtcttgttct ttgagatagt tgatttttgc attttcttgt gatggcgatg
      841 ccactaactg gctaggtggg aaaataacct cgatatctgc aaatttcttt ttcgggaagc
      901 cgttattatt taaaatcgtt attgcgcgaa caaaatcctc tttttccaca ctgagtgtca
      961 tatttccact tttgtccatt tctttggaaa cattgacatc attggatagc aacagtgctt
     1021 gcatctgatt agcttctttt tctgttaatc ctgtatacaa ttgctcttta catgccactg
     1081 tcaaacaaat agcagccaat aaaaaaaggt ttttaatgtg ttttttcatt tttattgcat
     1141 cgaaactaat tttgtaaccg atgttgaaag gaggcttgct aacttagata cgacatcaac
     1201 gttgacgttt gcgttgagca cattcttttg taaggtgagc acgtcttctg gcgagagccc
     1261 acctgcagat agagcatcga aactgagatc gctgttcatt gttgcaagat gtctcttttt
     1321 aatttcttca atcaacactt gttctggaga agacactccc gctgttttag ggagtaattg
     1381 atcaagaaac tgagccgcac ctgccgcaga gggggctgag gctaacgagg tatgctcatc
     1441 aacgaaattt gtttggggcg ctttatttaa ttgaatatta atgttttccg attgattaat
     1501 gttgttcata tttactgtta gcctcttttt atgaaagagg caagggcata atacatccat
     1561 tacctctatc ttttatttat gcgatgggcc ggttaggcat atttcatcgc taatgcaccg
     1621 ccaaccgcag taagagtacc tagtacgcca gaagtaatac ccagggctaa gtattctgtg
     1681 ctgctcgtct ccgagcatgc cgctgcaata cctgtaccta gagctgccag agccgtagtg
     1741 gtaagtgcta atccggctat aactctaacg gtgcgatttg ttccattttc tggtttaatc
     1801 actcctgtct tctcatccac tgagttattt ccactgagtg aggtcgccag cggcattact
     1861 gcaccagaag gacttaaatt tgctgcttcc attgatcttt ctccttttgt ctaatttaga
     1921 catttacctg gttataaata accaaattat cccataggca actattatat tattta
//
LOCUS       DAZFDC010000306         1962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFDC010000306 DAZFDC010000000
VERSION     DAZFDC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.308"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1500
                     /locus_tag="ACHJ48_005016"
     CDS             <1..1500
                     /locus_tag="ACHJ48_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4710361.1"
                     /translation="KGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEP
                     ATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETA
                     ARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKG
                     EIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNKKLENQSAAIQQIQK
                     VQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPA
                     NGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNV
                     NANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRV
                     YDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSG
                     VIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
     gene            1568..>1962
                     /locus_tag="ACHJ48_005017"
     CDS             1568..>1962
                     /locus_tag="ACHJ48_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4710362.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFYGDYL"
BASE COUNT          489 a          467 c          561 g          445 t
ORIGIN      
        1 aagggaacgg atgccagact ggtgaccacc gccatcaccg cagacacgga gcaccgtttc
       61 agcggcctgc cgctcgggga atacaccctg acggtgcggg cgataaacag ctatggccag
      121 cagggtgaac ctgccaccac caccttccgg attgccgcac cggcagcacc gtcgcggatt
      181 gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgccgt ttatgacccg
      241 acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag gcaggttgaa
      301 accgcagccc gctatcttgg ctcggcgctg tactggatag ctgccagtat caatatcaaa
      361 ccgggccatg attattattt ttatatccgc agtgtgaata ctgttggcaa atcggcattc
      421 gtggaggctg tcggtcgggc gagcgatgat gcggaaggtt acctggattt tttcaaagga
      481 gaaatcggga aaacacatct ggcccaggag ctgtggacgc agattgataa cggtcagctt
      541 gcgccggacc tggctgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcag
      601 accgtcaata aaaaactgga aaatcagagt gcggcaatcc agcagataca gaaagttcag
      661 gttgatacaa ataataacct gaacagcatg tgggccgtga aactgcagca gatgcaggac
      721 ggacgccttt atattgcggg tatcggtgcc ggtattgaga atacgccagc aggaatgcag
      781 agtcaggtgc tgctggcggc agacaggatt gcgatgatta atcctgcgaa tggcaacaca
      841 aagccgatgt ttgttggtca gggcgatcag atatttatga atgaagtgtt cctgaaatat
      901 ctgacggctc ccaccattac cagcggcggt aatcctccgg cattttccct gacaccggac
      961 gggcggctga cggcgaaaaa tgccgatatc agcggtaacg tgaatgcgaa ctccgggacg
     1021 ctcaacaacg tcacgattaa cgagaactgt cgggttctgg gaaaattgtc cgcgaaccag
     1081 attgaaggcg atctcgttaa aacagtgggc aaagctttcc cccgggactc ccgtgcaccg
     1141 gagcggtggc catcaggaac cattaccgtc agggtttatg acgatcagcc gtttgaccgg
     1201 cagattgtta ttccggcggt ggcattcagc ggcgctaaac atgagaaaga gcatactgat
     1261 atttactcct catgccgtct gatagtgcgg aaaaacggtg ctgaaattta taaccgtacc
     1321 gcgctggata atacgctgat ttacagtggt gttattgata tgcctgccgg tcacggtcac
     1381 atgacactgg agttttcggt gtcagcatgg ctggtaaata actggtatcc cacagcaagt
     1441 atcagcgatt tgctggttgt ggtgatgaag aaagccactg caggcatcac gattagctga
     1501 attttataac ccagatacgg gcgccagaaa tggcgccttt tttattgcag aaaagcgaga
     1561 ggtaattatg cgtaaattat gtgctgttat tttgtccgca gtagtctggc aggtcgccgc
     1621 tgctacgcca gcgagtgcag cagaacatca gtccacgctg agcgcggggt atctccatgc
     1681 ctcgacgaac gttcccggta gtgatgatct gaacgggatt aacgtgaaat accgttatga
     1741 gtttatggac gcgctggggc tgattacgtc cttcagttat gccaatgctg aggatgagca
     1801 aaaaacgcgc tacagcgata cccgctggca tgaagattcc gtgcgtaacc gctggttcag
     1861 cgtgatggcg gggccgtctg tacgcgtgaa tgaatggttc agcgcgtatg cgatggcggg
     1921 tgtggcttac agccgtgtgt cgactttcta cggggattat ct
//
LOCUS       DAZFDC010000307         1950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFDC010000307 DAZFDC010000000
VERSION     DAZFDC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.309"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..786)
                     /gene="nleH1-2"
                     /locus_tag="ACHJ48_005018"
     CDS             complement(<1..786)
                     /gene="nleH1-2"
                     /locus_tag="ACHJ48_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFO4710363.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDNGNVIGIRMNKINGES
                     LLDIPSLPAQAEQAIYDMFDRLEKKGILFVDTTETNVLYDRMRN"
     gene            complement(893..964)
                     /locus_tag="ACHJ48_005019"
                     /pseudo
     CDS             complement(893..964)
                     /locus_tag="ACHJ48_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            958..1950
                     /locus_tag="ACHJ48_005020"
                     /pseudo
     CDS             958..1950
                     /locus_tag="ACHJ48_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          585 a          465 c          400 g          500 t
ORIGIN      
        1 atttctcata cgatcatata aaacatttgt ttctgttgta tcaacaaaaa gaattccttt
       61 tttctccagt ctgtcaaaca tatcgtaaat agcctgttca gcttgtgctg gtaatgatgg
      121 aatatccaaa agagattccc catttatttt attcattcta ataccaataa catttccatt
      181 atcattatat attttctctg cactcccgga accataatac tgattgaaac aacgaacttc
      241 gcttgtcacc tcttcatggc tttgagatat agtaaacatc ttcaacactt ttgttgtatc
      301 ttccatatct tcatacacga cagcattacc acctttacca ataacattac ctggcacggg
      361 tgatttattg ttgctaaccc tcggcaactc tgattgagta taatccgctg gaggtaactc
      421 cggctgcgca taatccactg gaggtaaatc aggtctgttc gaatgaacag agcctctttc
      481 tatagtcgtg ctcactggcg atgtattcag catagcctca atttttctgc taatctctcc
      541 cttaggccat cccaacctgt gcaacagatt agtaaaacaa ccactatgac tttctcttgt
      601 aacgcaaaac ttattatcag tgaccacaac acgatatgtt ctgttgccaa ccgttacttg
      661 cgtcccgcta tcagagtgaa cagcagcatc ccttacagag gataaaacac gattatcagg
      721 cgaagtcagg tttctggtta aagaattcca tgaacatccc aaatttatag aagagggcga
      781 taacatacat ttcaaccttc aaaataaacc tatctaatta ttcctaacag acatccccca
      841 tacatgacaa caaaaaccgg agccggactc cggtttttgt gaagctatcg ggttacttca
      901 tttcgccaat attttcccac ttcccgtcag cacgcaggat ttgcagcggt cttactacac
      961 actgacccga ggacgaaaaa agagttcggg ctgggaagag acagaagagt agcaatttca
     1021 gaagcattca caccaacatt gagcttttca gcgaaagtgg gcacgaatca ttgctggaca
     1081 gggttaaagg cactgactca atcactcttc atacgtggct tgatcgatat gaaacaatcc
     1141 tcagctagag ggggagcaag ccgaaaacat tactcgacta cgccagcaaa atcagggcaa
     1201 ttcgaagaaa attgccggac aaaccgctca ctgacatatc aacgaaagag gtggcagcaa
     1261 tgctaaacac ctacgtcgca gaaggtaaag cggtttccgc aagagtaatc aggtcaaccc
     1321 ttgttgacgt ttttcgaggg gcaatagccg aggggcatgt ggcaacgaat ccagtaacaa
     1381 caacccgtgc agcaaagtca gaagtaaggc gctcaaggct gacagctaat gagtatgtcg
     1441 ctatttacca tgctgctgag cacctcccca tctggctgag gctgtcaatg gatttagctg
     1501 tcgttacagg gcagagagtg ggcgatttgt gcagaatgaa gtggtcagac ataaacgatg
     1561 gtcatcttca cattggacag agtaaaacag gagccaaaat tgccattccg ctggctctaa
     1621 ccattgacgc actcgacatc tcactggttg atacactaca gaaatgcagg gaggccagca
     1681 gcagtgaaac aataatcgca tcaacctatc acgaaccact ttctccagcc acagtatcac
     1741 ggtatttaac aaaggcgcga aatgcatccg ggatctcgtt tgatggagac ccaccgacat
     1801 ttcatgaact acgtagtctg tccgcgaggc tatatcggaa ccagattggc tacaagtttg
     1861 cacaacgtct tcttggacat aaatctgatt caatggcggc gcattatagg gacagtcgcg
     1921 ggcgggagtg ggacaaaatt gaaatcggat
//
LOCUS       DAZFDC010000308         1929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFDC010000308 DAZFDC010000000
VERSION     DAZFDC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.310"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(473..>1929)
                     /gene="nleL"
                     /locus_tag="ACHJ48_005021"
     CDS             complement(473..>1929)
                     /gene="nleL"
                     /locus_tag="ACHJ48_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO4710364.1"
                     /translation="LNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTINLGGCYID
                     LSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISLDELAAFRP
                     YVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGM
                     YFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLC
                     DGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDG
                     EVQNTFGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEALNSN
                     KSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLG
                     GVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRN
                     NVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          691 a          408 c          252 g          578 t
ORIGIN      
        1 atatcaattc ataaaatatg attctccacg atttgcccag aaataacgaa cataattttc
       61 gttgtaatta aaatcatatt tcatctatag tgaccattca cagcaaaaca aaatcaatta
      121 tcacattcaa aaaaatactg acaacaaaga tactcttcag acaaagttgt catatgtaaa
      181 ttttccctta aaacgttgct caagaaaaat ataaaaatca tatgccctaa tcaacatcct
      241 caagcactac gatatatgca gtatcctatt catatattac caccatatca gacaaatcga
      301 atcacaacga gttctatcgt actctttcat cttcgacact gtaaatttta agaacattcg
      361 gctcatgcca ctaaaccaag acataataac aacggagcaa taaatgttcc cactgaacat
      421 gtgcaaacct aaaaagacag tgttacaata atattcatcc atggtctcta aatcaacgcc
      481 acgcaacagg ataatacaat gaaaatattt caggaaaaga ttcttttgca tgctccgtta
      541 gcatatcagt cagcacagcg gtacaagtaa aaacattatt tcttccttgt agcctgtcta
      601 aaatatcatt gtaataataa actgagccaa atacctgagg atctgtttta taagcttgtt
      661 caatcaatcc atttgcatat ctccgtagaa tctcaggaga atcatactct gtaccaaaca
      721 tatcactcga tgagtattta cagaaaaccc cccctaaaca aaataatatt tcagcctgtt
      781 ttcttagtgg catatctttc aatacatggc tccccataat catatctaca tgctgagcgt
      841 tcagttccca accatcaaga aagggattcc agacacaggc aagtttcctt tgctgatcat
      901 cagcaatcat tttataatct gatttattgg aattaagtgc ctcaataaat ctctctttga
      961 gttcattatc agaaatgagt atatccagaa taccagaaac gaaattctgg gaaaaagctt
     1021 tatgataagg aatactgaaa attggaaatg attcagaaaa aagattgctc aatgaatacc
     1081 caaatgtatt ttgaacttct ccatccttat ataaaaaaaa ggacatccaa ttagttgatg
     1141 tattaggaga taacatatca accatatgac tcaaagaaca catcattgcc ataccatcat
     1201 cctgtgatga taacaaaacc cagttataag ccaaatcgga atcatcatcc cagtcaggct
     1261 tcccagagcc atcgcacaat ccaaaatcac tctgctgtgt gacaggataa actttttcat
     1321 tttttaaata tacttcatag agttccctaa atcgttcctg cattccatca cgccgtgaac
     1381 cattcattac aatttgtata aaggaactat tataagatac catcgcacct ggatgtcgct
     1441 caaaatacat ccctgcctgc aataaactac cattctccat atacatcatt attgaattat
     1501 taaaccaaga cattatagta tcaaaaaagt catcacatat aggctcgagc cattgctgta
     1561 tatttgcgtt gttcaaataa ggatgaatac taaatgaatc agccaaagac atcttaacat
     1621 agggccggaa cgccgctaat tcgtcaagag atattttttt gataagttct tcagcggccc
     1681 ttactttatc atggttatac ttatcatcag atgtattcat agtcaataaa attgatttat
     1741 ttgattttgt atagttatcc aacacagata atatattttc ctgtatgtca gatagatcta
     1801 tgtaacaccc tcctagatta atggtattac ttttttcact aaagagctcc tttacatcac
     1861 ctaagatagc accacttaaa accattccag gaattataat agctcctgtt aaatcagcct
     1921 cgttcaaaa
//
LOCUS       DAZFDC010000309         1913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFDC010000309 DAZFDC010000000
VERSION     DAZFDC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.311"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..554
                     /gene="espM1"
                     /locus_tag="ACHJ48_005022"
     CDS             <1..554
                     /gene="espM1"
                     /locus_tag="ACHJ48_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO4710365.1"
                     /translation="GISYHKDNSFRGTIRGKNDEVVKCSMGERSIRFNVNKFSGCILE
                     TVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEERKMVFSLVSKEFE
                     LTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYIANVLADKFYQKHI
                     APGVDIVKLRNEIPGYMSRVIQG"
     gene            complement(773..1829)
                     /locus_tag="ACHJ48_005023"
                     /pseudo
     CDS             complement(773..1829)
                     /locus_tag="ACHJ48_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          511 a          396 c          465 g          541 t
ORIGIN      
        1 ttggtatcag ttatcataaa gataattctt tccggggcac catccgtggg aagaatgatg
       61 aggtcgtgaa gtgttcaatg ggagagcgct ctatccgctt taatgttaac aaatttagcg
      121 gctgtatact ggagacggta agcaggcaga gtactaaaga tattcatgga tgggtaagtg
      181 atgaacggac agtatatccg tcaagggtta tcaaccaaga gattgataat tgctgccttc
      241 aaaaaaatgc aaaaatttct tctgaagaga ggaaaatggt cttttctctt gtaagtaagg
      301 agtttgaact aactcttgat gttaaagcgg cacaaagttc tattaatcat ataataatag
      361 gaaatgcttc ttttggcaaa aaaatggatg ctctttgcga tggtatgagc cgagctgtaa
      421 aaaacagtac aacagattac atagcaaacg tgcttgcaga caagttttat cagaaacata
      481 tcgccccggg tgttgatatt gtaaaactac gaaatgaaat cccaggttat atgagtcgtg
      541 ttatacaggg gtaaggttgg aggaggctat gcgccagtat cattggtttt catcctgtaa
      601 aaagatatga gtaaaatata caacaggcgt tacactatat gtatgtatcc aaataactca
      661 tagttcacct gtacagttat aaatcgtaaa gaaaattgca gcgcgtcgta cgcagaaacg
      721 tgctgtgatt gactatttat agctttgtca caacgacaag attcagtagt gtttacacgg
      781 gcgggagaaa tgacggaata actaaatagc tgcgcctaat atcgcacatt tttgccagcc
      841 catgtttgcc ttcggggaat ggactgacgg attccataaa atggcgaact ttttcaatag
      901 ttgccacatt gagcggcact gatgattacg cgttattgtg gcgtgaagta tctgccatag
      961 ctattcaaaa tgattcaccc atggagatta aaccggctga taaatgactc tgcacttcgg
     1021 tttatccttc agccagcgct gggtttcccg gcctttgtgg ataatgtagt tgtcctcgag
     1081 cagcgtgatg gttttcgtcc gacagtatgt agctttaagc cgcttcagca gtctgatgaa
     1141 cagcgccgaa cttttgctgt tggcgcccac acagctgact taacctgtcc cgctgtgcag
     1201 cgctacgtcc agagaatatt tttcattttg ttccggcgtg acccctcgtt tttgcttccc
     1261 gagcagtttc cagtccgcac cgattttggg attaagatgg ataaatactt catcttcata
     1321 aaagaccgga tgctctgtgc tgcattcgtc cagtgcgtta tggattgttg ccatcttttt
     1381 atctttaggc ggggcacgga tacgtagagt tggcgcagcc cttcgccaca caagccccac
     1441 agacgacaac cagcggcgaa tggttccagc atgtaacagg cagccggtta tctcattgat
     1501 ttttattgtc agcagttctg tactccagcg tgaataattg tagccaaagt cgcgagtgaa
     1561 tattttacct gttcacgtaa tagtgtgtag atatgcgcaa acggccagcg gcgggctcgc
     1621 tcggcaggta atgatttcag tccctcaaca cccgactgtg tgaactagtt aatccagcgc
     1681 ccaacagagg aacgggcgca gcatagtgtg cgggcaacgt cgctgacacg gtcgccccgg
     1741 tgcagcatca gcatggctgt cagcctttta tggatggctt tctgtattag gcgtcgttcg
     1801 tcacgggaaa ttggtggtat gatcgacatt gctcagtccg gttggtgatt tgtattgatt
     1861 tggcgattga tcagatcgca caatcgggtt gagttccctc taaatgatct act
//
LOCUS       DAZFDC010000310         1910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFDC010000310 DAZFDC010000000
VERSION     DAZFDC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.312"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            133..1509
                     /gene="ygeH"
                     /locus_tag="ACHJ48_005024"
     CDS             133..1509
                     /gene="ygeH"
                     /locus_tag="ACHJ48_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO4710366.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLKTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLRRFNPDYFVTGRINQNNAVNTLYIELTDAKNLFLIASNHLPVDE
                     LHNTSQFIIENILQTVHNPQRSVRLTKQDQGYKNHYLSDEMLAGKKELYEFTPESIYR
                     AMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKKNMKLYYKETESGSHRVIIDNILKLK
                     QLTRICMR"
     gene            1676..1894
                     /locus_tag="ACHJ48_005025"
     CDS             1676..1894
                     /locus_tag="ACHJ48_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710367.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDKVQIQAF
                     IVSTWMASFQNDMYSEDNPISPYHKIEW"
BASE COUNT          704 a          318 c          319 g          569 t
ORIGIN      
        1 aattagtgaa cacaaccata gatatgtcga atgtaaactc ctgtagcgtt aatgacacat
       61 aaatatgaat agccataatt tctattgctt atgcaggatg caagaaacca attttttcat
      121 agaggttaac taatggactt agaaaataaa ttctcatatc attttcttga gggattaacg
      181 ctcacggaag atggaattct tactcaagga aatgagcaag tttatattcc accgaaagag
      241 ttaggtgtat taatagtatt acttgaatcc gctggtcatg tcgtactgaa agatatgatc
      301 atcgaatcag tatggaaaaa tattattgtt agtgatgagt ccctgacaag atgtatctat
      361 tctttgcgct gcatttttga aaaaattggc tatgatcgtt gcatagaaac aatctaccgg
      421 aaaggttatc gtttcagtgg gcaggttttc aaaactaaaa taaatgaaga taacacttca
      481 gactattcta tcgctatatt ccctttcact acttcattga aaacactgga tccattaata
      541 cttaatcagg aattagtaca aatcatttca aataaaaaaa tcgatggcct ctatacctat
      601 ccgatggctg cgacaaattt ttgtaatgat cacatatcgc aaaattcatt tttgagaaga
      661 ttcaatccag attatttcgt tacaggaaga ataaaccaga ataatgcagt gaacacttta
      721 tacattgagt tgaccgacgc taaaaacctt ttcctcatcg ccagtaatca tctacctgtt
      781 gatgaactac ataatacatc acaatttatt atagaaaata tccttcaaac agtacataat
      841 ccacaacgat ctgtaagatt aactaagcag gaccaaggat ataagaatca ttatttatca
      901 gatgaaatgt tagccggaaa gaaagaactt tacgagttca cccctgaaag catttacagg
      961 gccatgacta tatttgatgg attacaaaat aaaagtgata tacagacgct aaaaacagaa
     1021 tgttattgcc ttctagcgga atgccatatg tctttggcac ttcatggaaa aagtgaactt
     1081 gaacttgctg ctcaaaaagc attagagctt ttagattatg tatcagacat aaccactgtc
     1141 gatggaaaaa ttttagctat tatgggactg ataactggtc tgtctggaca agcaaaagta
     1201 tctcatatct tatttgaaca ggctaagata cactcaactg atatagcctc tctctactac
     1261 tatagggcac ttgtcaactt tcataatgaa aaaattgaag aggcaaggat ttgtatagac
     1321 aaatcactac aactcgaacc cagaagacga aaagcagttg tgataaaaga atgtgtagat
     1381 atgtatgtgc ctaacccgct caaaaaaaac atgaaactct actataaaga aactgagagt
     1441 ggaagccatc gagttataat tgacaacatt ttgaaattaa agcagctgac gagaatttgt
     1501 atgcgataaa tttttattga cacaatttta ttatttagat gtatgcaact tgttatttaa
     1561 aaaacaaaga tcaaaatatc taagatattt accttattgc aagatattta aaatgctcta
     1621 gagttaaaag tatgatgtaa agaccattgg cctggacaat taaaaaggga gctttatgac
     1681 taatccaatc ggtatcaata atttatctca aagttcaaat atagcgaatg caacgggtga
     1741 tgaagtcgtt tcattagata aacacatcaa caccagtgct actgataaag ttcaaataca
     1801 agcatttata gtttcaacat ggatggcgtc ttttcaaaat gatatgtatt cagaagataa
     1861 tcctatctca ccttaccata aaattgaatg gtagacgatt gaccaaataa
//
LOCUS       DAZFDC010000311         1894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFDC010000311 DAZFDC010000000
VERSION     DAZFDC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.313"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..641)
                     /locus_tag="ACHJ48_005026"
     CDS             complement(<1..641)
                     /locus_tag="ACHJ48_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO4710368.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSKPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIART"
     gene            910..1026
                     /locus_tag="ACHJ48_005027"
                     /pseudo
     CDS             910..1026
                     /locus_tag="ACHJ48_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1131..1207)
                     /locus_tag="ACHJ48_005028"
     tRNA            complement(1131..1207)
                     /locus_tag="ACHJ48_005028"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1171..1173),aa:Arg,seq:tct)
     gene            complement(1221..1297)
                     /locus_tag="ACHJ48_005029"
     tRNA            complement(1221..1297)
                     /locus_tag="ACHJ48_005029"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1261..1263),aa:Arg,seq:tcg)
     gene            complement(1305..1380)
                     /locus_tag="ACHJ48_005030"
     tRNA            complement(1305..1380)
                     /locus_tag="ACHJ48_005030"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1345..1347),aa:Ile,seq:cat)
     gene            complement(1409..>1894)
                     /locus_tag="ACHJ48_005031"
     CDS             complement(1409..>1894)
                     /locus_tag="ACHJ48_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO4710369.1"
                     /translation="PLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDE
                     RILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSR
                     LLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLD
                     Y"
BASE COUNT          479 a          513 c          461 g          441 t
ORIGIN      
        1 gtgcgcgcaa tcaggtcctg atataacggt ttacccacac cccagcgtgc cgaatcctgg
       61 ctggcccccg tgtccgcact gaatgtcccc tcagcgccct gggtgaatgc agaaccacca
      121 cgacagcatg gtaccagcag gatccccgcg ttattcggga tatacggaag cagttttttg
      181 gcaatatgta agccctggcc gacacagccg tactgccctt tgctcaggtc tgccttcgga
      241 tgattcagcg tactcatatc ctgcacatca tgcaggcagt ggtcggccgg aatgatgtcg
      301 ttatacgtac aactctctcc acccggagtt accgtgctgc ggcgcgccag ctgtttaatg
      361 cgcggatccg gagcatcgta agaatccggt aacggaagcc cttcaccgta ggccatgccg
      421 ttggactgtc cggcaagcac aaccacgtag aaccagtccg gcttagatga agggccgacc
      481 tgtggatctc cttcaatagc caccgcctgc atcagtgtgt acggcgtaat ggcaaccggt
      541 ccgccgtatg gctgccagcc ctctttcagt ttgtgtgtca gcttttccgc aaggtctgac
      601 ggcgacgccg ccctgacaac atcatagtgt ttaaatgcca tgaatcctcc cggccgggat
      661 aatattgtga gtaaaatgag gagcgggctg aagtccggaa gttacaggac aatggcagaa
      721 gagagacaac agcccgcaat acgaaaaagg ccgcgctatt gcgcagagtg attactgtcg
      781 gatattattc gccagctgaa atattacttc acgttttgtt gtttattcct tgccgcccgc
      841 gtctccctgc gcgggctttt tttgtccata agaaagcccc tccggagagg ggctggagag
      901 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      961 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
     1021 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
     1081 tgagacattt aaaaaaaagg cccgcaaaag cgagccaggg aaaataagtg tggcgcgttg
     1141 tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc ggctgagcta
     1201 acaacgcagg atacagataa tggaccgcct tcggggaccc gaactccgcg caaccagctt
     1261 cgaaagctgg cgctctttcc tgatgagcta atggcggtat gtgatggtgg cccttgctgg
     1321 atttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggct
     1381 gggtcaaaaa ataataatca gatgaaaatc aataatcaag cccttgcctg gatacatatc
     1441 tgtctggcgg gaagccataa tagcggtgaa atacagaaat aaagtaggac cagcttgaat
     1501 aaccgcattt ttctgctaca gcctgtccat atccatgccg ggaacataac atattgacag
     1561 caacacgcat ccgctcttcc agcaacaagc gactgaacat gtgcccttca tttttcagtt
     1621 ttatctttaa caaactctca ctcatatgca ggcgtaacgc aatcgcacca agcgtccagc
     1681 ttgctgatat atctgtctga attatcgccc tgactttggc acttatactg gataaacatc
     1741 cacttaaaaa taataatatc cgttcatctg attcaaacag cgacaggcat gccatcataa
     1801 gaaacatatc cgtggtatct ccggaaaatc tctggctggt aattaaagcc tcagccaacg
     1861 cagggttgtt gggttccagt gacagataaa gcgg
//
LOCUS       DAZFDC010000312         1892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFDC010000312 DAZFDC010000000
VERSION     DAZFDC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.314"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1892
                     /locus_tag="ACHJ48_005032"
                     /pseudo
     CDS             1..1892
                     /locus_tag="ACHJ48_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          654 a          378 c          358 g          502 t
ORIGIN      
        1 tatcaacttt tcacgcagaa aaagtcatta atgatctgtt aaaaaacgat tataaaattt
       61 ccctttctcc agatacaact taccgagagc tgaataaagc tgcacagcgt agcattacag
      121 cgccagacag gataggagaa gggaaaatat gggtttatca acgagataca atggttgaaa
      181 gaggtgataa cagcggtgtt catcagtatg gtccagctga acatttcact cacattatat
      241 ctgacaaacc gtccccaaaa gataagtatg ttgcatatgc tattaacatt cctgactatg
      301 agctagcagc cgatgtatat aatattaacg tgacgtcacc ttccggacag caagaaacat
      361 ttaaaatatt aatcaatcca gaacatctac ggcaaacact tgagcgtaaa tcccttactg
      421 ctgttcagaa atcacaatgt gaaatcatca cccccaaaaa acctggcgaa gcgattcttc
      481 atgcttttaa tgccacctac cagcaaatca gggaaaatat gtctgagttt gcacgttgcc
      541 attatgggta tatacaaatc cctccagtga caactttccg cgccgacgga ccagaaactc
      601 ccgaagaaga aaaaggttac tggtttcacg cttatcaacc cgaagatctt tgtaccatcc
      661 ataatccaat gggagatttg caggatttta tcgcattggt taaagatgct aaaaaatttg
      721 gtatcgatat cattcctgat tataccttta actttatggg aatcgggggt agtggtaaaa
      781 atgacctgga ttatccctct gctgatatac gagcgaagat cagtaaagat atagaaggtg
      841 gtatccctgg ctattggcaa ggtcaggttt tgattccatt tactatagat ccagtaacaa
      901 aagaacgtaa acaaatccat ccagaagata tacatctcac agcaaaagac ttcgaagcaa
      961 gtaaagataa tatttctaag gatgaatggg aaaacctcca tgcattaaaa gaaaagcgtt
     1021 taaatggaat gcctaaaacc ccccccaaaa gtgactaggt tattatgttg caaaatcaat
     1081 acgttcgtga aatgcgaaaa tatggcgtac gaggtttacg ttacgatgca gcaaaacact
     1141 caaaacatga acaaatagaa agatcaataa ccccaccgct taaaaattat aatgagcgat
     1201 tacacaatac taacttattt aacccaaaat atcatgaaaa agccgttatg aattacatgg
     1261 aatatctggt aacttgccag ttggatgaag aacaaatgtc atcgctgctt tatgaaagag
     1321 atgatttaag cgccattgat ttttcattgc tcatgaagac gataaaagct ttttcatttg
     1381 gtggcgatct ccaaaccctt gcatcaaaac cgagttccac aatctcaagc atcccatcaa
     1441 aaagacggat attgattaac attaaccacg attttcctaa caatggcaat cttttcaatg
     1501 actttctatt taaccatcaa caagatgaac aattagcaat ggcatatatg gccgctctcc
     1561 cgttcagcag gcctttagtt tactgggatg gccaagtatt aaaatcaacg actgaaatta
     1621 aaaattatga tgggtcgacg cgtgtcggcg gtgaggcgtg gcttaataaa ggttgctcta
     1681 cctatcagca gctctacaat gaattccacg cattatatat agataaagca ggaatatgga
     1741 gcgcatttga gggtgtattt gcaactaaaa acgttctggc ctttagtcgt ggggattcgg
     1801 tgaacattaa tcactctcct catgatggac tagttataat aaataaagga aacgaagaag
     1861 ttgaaggtgc ctggcctaac aaattgcaac ct
//
LOCUS       DAZFDC010000313         1889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFDC010000313 DAZFDC010000000
VERSION     DAZFDC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.315"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            149..1819
                     /gene="tir"
                     /locus_tag="ACHJ48_005033"
     CDS             149..1819
                     /gene="tir"
                     /locus_tag="ACHJ48_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO4710370.1"
                     /translation="MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS
                     RALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQ
                     IGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGG
                     AGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQT
                     STSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATE
                     TATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAI
                     ENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQ
                     TTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSSIG
                     TVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLLGN
                     PSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV"
BASE COUNT          531 a          398 c          490 g          470 t
ORIGIN      
        1 ttttcttgca tcaaaaatca gattgtgatt tatttggtcg atgtcatgtt cagccgttta
       61 tcgactacgt gctgtatacc atacattatt atgtaatagc aaaataaagg attgttatct
      121 atttacataa aataaaaagg agatatttat gcctattggt aatcttggtc ataatcccaa
      181 tgtgaataat tcaattcctc ctgcacctcc attaccttca caaaccgacg gtgcaggggg
      241 gcgtggtcag ctcattaact ctacggggcc gttgggatct cgtgcgctat ttacgcctgt
      301 aaggaattct atggctgatt ctggcgacaa tcgtgccagt gatgttcctg gacttcctgt
      361 aaatccgatg cgcctggcgg cgtctgagat aacactgaat gatggatttg aagttcttca
      421 tgatcatggt ccgctcgata ctcttaacag gcagattggc tcttcggtat ttcgagttga
      481 aactcaggaa gatggtaaac atattgctgt cggtcagagg aatggtgttg agacctctgt
      541 tgttttaagt gatcaagagt acgctcgctt gcagtccatt gatcctgaag gtaaagacaa
      601 atttgtattt actggaggcc gtggtggtgc tgggcatgct atggtcaccg ttgcttcaga
      661 tatcacggaa gcccgccaaa ggatactgga gctgttagag cccaaaggga ccggggagtc
      721 caaaggtgct ggggagtcaa aaggcgttgg ggagttgagg gagtcaaata gcggtgcgga
      781 aaacaccaca gaaactcaga cctcaacctc aacttccagc cttcgttcag atcctaaact
      841 ttggttggcg ttggggactg ttgctacagg tctgataggg ttggcggcga cgggtattgt
      901 acaggcgctt gcattgacgc cggagccgga tagcccaacc acgaccgacc ctgatgcagc
      961 tgcaagtgca actgaaactg cgacaagaga tcagttaacg aaagaagcgt tccagaaccc
     1021 agataatcaa aaagttaata tcgatgagct cggaaatgcg attccgtcag gggtattgaa
     1081 agatgatgtt gttgcgaata tagaagagca ggctaaagca gcaggcgaag aggccaaaca
     1141 gcaagccatt gaaaataatg ctcaggcgca aaaaaaatat gatgaacaac aagctaaacg
     1201 ccaggaggag ctgaaagttt catcgggggc tggctacggt cttagtggcg cattgattct
     1261 tggtggggga attggtgttg ccgtcaccgc tgcgcttcat cgaaaaaatc agccggtaga
     1321 acaaacaaca acaacaacta ctacaactac aagcgcacgt acggtagaga ataagcctgc
     1381 aaataataca cctgcacagg gcaatgtaga tacccctggg tcagaagata ccatggagag
     1441 cagacgtagc tcgatggcta gcacctcgtc gactttcttt gacacttcca gcatagggac
     1501 cgtgcagaat ccgtatgctg atgttaaaac atcgctgcat gattcgcagg tgccgacttc
     1561 taattctaat acgtctgttc agaatatggg gaatacagat tctgttgtat atagcaccat
     1621 tcaacatcct ccccgggata ctactgataa cggcgcacgg ttattaggaa atccaagtgc
     1681 ggggattcaa agcacttatg cgcgtctggc gctaagtggt ggattacgcc atgacatggg
     1741 aggattaacg ggggggagta atagcgctgt gaatacttcg aataacccac cagcgccggg
     1801 atcccatcgt ttcgtctaaa tatatccata atcattttat ttagagggag ggagggggga
     1861 agtctaacta acgtcaattt ttcagggaa
//
LOCUS       DAZFDC010000314         1849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFDC010000314 DAZFDC010000000
VERSION     DAZFDC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.316"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..115)
                     /locus_tag="ACHJ48_005034"
     CDS             complement(<1..115)
                     /locus_tag="ACHJ48_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710371.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGH"
     gene            complement(99..>1849)
                     /locus_tag="ACHJ48_005035"
     CDS             complement(99..>1849)
                     /locus_tag="ACHJ48_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4710372.1"
                     /translation="HRETARSFGGAGSTAGYEQVTAYTLTGQLQSRHLNLPQLDCDYD
                     WNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELH
                     PDSTLTAWPDNRIAEDAHYVYRYDEYGRLTEKTDRIPEGVIRMHDERTHHYHYDNQHR
                     LVFYTRIQYGEPLVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTVQTGTTRIQTVYRPGSFTPLIRIETENGEREKAQRRSLAEKLQQEGSEDGHGVV
                     FPAELVRMLDRLEEEIRADRVSSESRAWLAQCGLTVEQLEKQVEPEYTPARTLHLYHC
                     DHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQHDEESGLYYNR
                     HRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSG
                     VPGLIVANSITNSAYQFGYDMDAIVGGAHNGAADAMRYCYLMCRMTKTFGSTIADVIG
                     KNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNIGQLFGLDGIKA
                     DNPIKAKQGSSDASNY"
BASE COUNT          356 a          569 c          450 g          474 t
ORIGIN      
        1 tatgtccata aaatacgatg tcagaatttg aattgttatc aaacccaagg taaacaacaa
       61 ataaacaaag ggtaattaaa aaaatgacaa gtttgcctct aataatttga agcatctgaa
      121 ctcccttgct ttgcttttat tggattatct gcttttatac catctaaccc gaagagttgc
      181 ccaatgttat atttttcaat acatgcatcg ctacatttgg cagaaaaatc gccacaagca
      241 ataccgacag tgttattttt aagatccatg attctttctt tagctggctg accttgtcta
      301 tccccagccg cctcatgatt tttacctatc acgtcagcta ttgttgatcc aaatgtctta
      361 gtcattcgac acatcaagta acaatatctc attgcatcgg cggccccatt atgagctccg
      421 ccaacaatag catccatatc ataaccgaac tggtaagcac tgttagtaat gctgtttgca
      481 acaatcaatc caggtacacc agatacggca taaattagtc cacctgatgt catattctca
      541 atcgcatcta accccattgg gtctatcact tgtatgggat tcaacggata ctgatacata
      601 ttccatccac ctctcaaccc aatcgggtcc ggggtgatat accgcccctg caacggatcg
      661 tagtaccggt gacggttata gtacagcccc gactcctcat catgctgctg gcccggcaga
      721 cggtacggct ggtgcaggtg atgcgggttc tcctcattaa gctggttgcc ccattcatca
      781 tattccgcgc tccacgccgt attgccgtct tcgctgataa gcgccagcgg cagtccccgg
      841 tggtcacagt ggtacagatg cagcgtgcgc gccggcgtgt attccggctc cacctgtttt
      901 tccagttgct ccaccgtcag tccgcactgc gcaagccacg cccggctttc actgctcacg
      961 cggtctgccc ggatttcttc ctccagcctg tccagcatcc tcaccagttc tgccgggaac
     1021 accacaccgt gaccgtcctc actcccttcc tgctggagtt tctccgccag gctgcggcgc
     1081 tgcgctttct cccgctcgcc gttctccgtt tcgatgcgga tgagcggtgt gaagctcccc
     1141 ggccggtata ccgtctggat acgtgtggtg ccggtctgta ccgtcgtcag cctgtcgccg
     1201 tcccacccgt accaggtcac ctccggttta cgcgacagcg acatccaccc cgtcaggtca
     1261 cgctcccgcc gccacacccg tttccccgtc cggcggccca gcgggtcata gaggtagcgg
     1321 ctctcgacca gcggctcgcc gtattgtatc cgcgtgtaga acaccaggcg gtgctggttg
     1381 tcgtaatggt agtggtgggt ccgctcgtcg tgcatccgga taaccccttc cggaatgcgg
     1441 tccgtcttct ccgtcagcct gccgtattca tcgtagcggt agacatagtg cgcatcttcc
     1501 gcgatgcggt tatccggcca cgccgtgagc gtgctgtccg gatgcagttc cgggtccggc
     1561 agccggtttc ctgccgggtc cgttgcatac gggatatcga tatccaggtt cgctgccgtg
     1621 gtgtggacgc ccgtcagcct tcccgtgtcg ctgtaacggt actcccggct ctcctgcggg
     1681 ccgctgatgc ggattagctg tccgttgtcg ttccagtcgt agtcacagtc aagctgcggg
     1741 aggttcaggt gcctgctctg tagctgccct gtgagggtgt acgccgtgac ctgttcgtac
     1801 cccgctgtac tgcccgcccc gccgaagctg cgggccgtct cacggtgca
//
LOCUS       DAZFDC010000315         1829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFDC010000315 DAZFDC010000000
VERSION     DAZFDC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.317"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1477
                     /gene="espN"
                     /locus_tag="ACHJ48_005036"
     CDS             <1..1477
                     /gene="espN"
                     /locus_tag="ACHJ48_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFO4710373.1"
                     /translation="LEFVHEINNISSARWMSKSEKNNKQKEAIEKFKKEVSHMNGGQQ
                     FIWGFDKVIQEGLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVP
                     GYNALSGVSSILHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEALK
                     HIEKNINDNIIYHPSNNANFYSVIESIDGNDFIYNEKQSKILEMKQDRGGNRYSAVDL
                     NSSKYGYYEKVGGGFYRYIESFNPISSETPNKIVYKGESVDLTKEPNSELYSGRYSIN
                     NKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQLREGDIGYDEDLLDIYDDSPV
                     LEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKT
                     AGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYI
                     TRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMVNASK"
BASE COUNT          593 a          285 c          394 g          557 t
ORIGIN      
        1 tcttgagttt gtccatgaaa ttaataatat atcatctgct cgctggatgt caaaatcaga
       61 aaagaataat aaacaaaaag aggcaataga aaagttcaaa aaagaagtat cccatatgaa
      121 tggcgggcag cagtttattt gggggtttga taaggttatt caagaaggct taagtggatt
      181 gattgagtta agtatcgata ttaatgatag tacaaatcat cgtgataagt cttctctttc
      241 tcctgatggg agagctgtgt tacatttttt aggtacaatt tggaatatgg cgatgggagc
      301 tgtacctggt tataatgcat tgtctggtgt aagtagcatt ttacatagtg ctatagtaaa
      361 agaatctagc aatatctgtg attatattca gggggctgta cgtattggaa tggactttgt
      421 tccaggcact cgctctgact tacatagccg ttcgctgcag ataaaatatg aggctttgaa
      481 gcatatagaa aaaaacatta atgataatat tatttatcat ccgagtaata atgctaattt
      541 ctattctgta attgagtcaa ttgatggtaa tgactttata tataacgaaa aacaatctaa
      601 aatattagaa atgaaacagg atcgtggggg gaatagatat agtgcagtag atcttaactc
      661 ttctaagtat gggtattatg agaaagttgg cggtggtttt tatagatata tagaatcctt
      721 taaccccata tcttcagaga caccaaataa aatagtctac aagggggaat cagtagattt
      781 aactaaggag ccaaattcgg aattatattc aggtaggtat tctataaata acaaacaggt
      841 taatgtttat ttctttcgtg acgctgatgg tacattttat aaatcagaag gtcttcatgg
      901 tgggggagtt attagataca tagataaacc gtattctcag ttaagagaag gagatattgg
      961 gtatgatgag gatttgttgg atatatacga tgattctccg gtgcttgaag acacgttgcc
     1021 tgctttatct tctgaaatag taccaactcc agaacatagt attaaacaaa tttattcgaa
     1081 aattaaggag gggcacatag aactgtccga ttcagacatc atattgtgtc gcggcacaac
     1141 cggtattcaa gctgaaaata tcgttgaata taaaactgct ggagggcttc ctgattcaaa
     1201 tccaaatgta aaagcaccag atgaatatat ggcacaacag caggtacgta ttggaagaat
     1261 attgcctgaa tacacatcgg atcttagcgt tgctgatcgg tttagtcgtg agcattatct
     1321 aatagttgtt aaagtaaagg caaaatatat cacacgagga agtgttacag agagtggttg
     1381 ggttatagat aagaccgcac ctgttgaacc acttgcgata attgatagaa cttttggtat
     1441 gaaggaaaat atctcaatgg taaatgcatc gaaatagttt ttttacaatc tatgtcctgc
     1501 ctcctctggt aaaaacgatg ctacatcttg gagatgttgc acggcagtac ggtgttgacc
     1561 gatatgtagt gaagtacaca tcggtcaacg aattaccatt gtcagcaata tcatcctgat
     1621 aaactcagta ctcgtgagcc gcttgatgac gggcggaata gcccaaaagg taaacgatcc
     1681 gcatggcagt ccgtcggaaa gctgctgctt catacgaccg cttaaaccgt cagttagtgt
     1741 cagtatcgct gaagatcagc ttcttttgct gatttactct gtttttacct gccctgatga
     1801 aatactcttt aatcataaca ttgattata
//
LOCUS       DAZFDC010000316         1793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFDC010000316 DAZFDC010000000
VERSION     DAZFDC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.318"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            185..286
                     /locus_tag="ACHJ48_005037"
                     /pseudo
     CDS             185..286
                     /locus_tag="ACHJ48_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(696..931)
                     /locus_tag="ACHJ48_005038"
                     /pseudo
     CDS             complement(696..931)
                     /locus_tag="ACHJ48_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1090..1740)
                     /locus_tag="ACHJ48_005039"
     CDS             complement(1090..1740)
                     /locus_tag="ACHJ48_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4710374.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSRELITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
BASE COUNT          553 a          403 c          297 g          540 t
ORIGIN      
        1 acagaattta cgatgctttt ttatacagat ctctcgtgtt tttaactgat tgattttgtt
       61 gaactctttt tttgttttgc aggagttact cctcttacct tattgtccac tcgatgacca
      121 acacattcac aacccaaacc agaatatcct atctatgaag catgtatccg cacgcagatt
      181 aattatggtt tacattgttc taccacaata gtcgcatcct aaatgggtgc ggttgaggat
      241 caccatcctt gaagaggatg cccgccactt taactgcatg acagggctat ttaaacaatc
      301 actaaaaatc gcccaagagc attctgtttc tataatgtat tccaagagat taaaaaataa
      361 tatcatacgt tattcggcag agttcgtatg tactacggcg tcccaaccat gtcccaaaac
      421 aaaaatttct gccctataca cgttccaaaa aattcaacag caaagagagt ctgctgacga
      481 gttgttgatt cactgattaa tacgtaaaat tgataactgc tggaaatcat acaatactcg
      541 tactttcgaa agttcgctaa ccaggtgcta cacgcgaggt gttgcagttt tctttacgat
      601 ttatcattca atcctaatcc atgcatgaca tgtggtgcct gtggggtgat cctacctacg
      661 taatgtggac acagccctaa tcaaggttct gtttttcaaa acagttccgt gctgagacag
      721 ccacaagtac tgtgtcggca ccaccgattg taatcacact agatctaatt aaacacagcc
      781 gtctgcatta tttcccgact cataaaatat tagccgtaaa tgcattcgac ttttagtgag
      841 tggaagaaac tttccgccct agcattatcg taacagcgcc cctttgcact catcctgtca
      901 tgcaggtatg ccgcttcaga agcgcctgat aatgcgttga aatcaggacg gtggctactt
      961 gctattcagt tcacgataca tcccttaagg accagcgaat agcaatgagt cactttatta
     1021 tcaagtttga tgaccgcttt gatgctaact tttaataaga gtctcttcac acaaaatcgt
     1081 gtaccgagtc tacactgaat aacaatcact cctcaccgta aagtgaccga tactgcaatc
     1141 aaactcacac tgctctctgg ccacaagcat tgataatgtt attagctctc tgcttagtgg
     1201 gtgatgcatc ccatcattaa ttatacgaga aagagccatt tcatcatata aactacatac
     1261 tctggaacct tcagcatttc ttaaaaaaac acctcgttga ggaattgcca gcgtaatcgg
     1321 gcactgtaat gcttcatcta tgtaagacat ttcttcaata ttaactaaaa atgaacatga
     1381 atgtaatcgc cgctgtaaca attcaattct gttatcatta ctatgcataa attcagaaat
     1441 aacctgcccc atatatgtac tgaatacatc aagaaatgaa ttgcctccat tcaattgatt
     1501 ttcaacagaa tcagccaatc taatgatatc ggctagttct gtatggctta aacccgaata
     1561 tagttcccct tgaacaggat ctacaataaa agcgtccata gcactctgat agcgaacaga
     1621 aaacgatctt tctcctattg tgagtctttc catttgatga tctctggtcg cacgttgtaa
     1681 aacttcaaca tcttgtgttg acaagtaatt tccagaagac aaattaaatg aaaatggcat
     1741 aattttatcc ctaaagcata tatcttactt ttcgataatt aacaaaaagc cat
//
LOCUS       DAZFDC010000317         1759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFDC010000317 DAZFDC010000000
VERSION     DAZFDC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.319"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..163)
                     /locus_tag="ACHJ48_005040"
     CDS             complement(<1..163)
                     /locus_tag="ACHJ48_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO4710375.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTA"
     gene            complement(144..1745)
                     /locus_tag="ACHJ48_005041"
     CDS             complement(144..1745)
                     /locus_tag="ACHJ48_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4710376.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTSDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          360 a          560 c          467 g          372 t
ORIGIN      
        1 cggcagtcag gctgtcgccg gatactgcat ccgtcaggcg gctgatccca agctggcctg
       61 caagcgcaca aaagaaaacc cgcgcatagg cgggttcaag catcagcggc tcattaaaag
      121 ccatgctggc aatatgcggg agattacgca gctctgctgt cactcttctc ctcctctgtt
      181 gattgtcgca gcccggattc aaatgccgca gccgcccagg cgggcggttt aagaccagct
      241 gcgcggcgct ccatcgtttc acggacctgc tgggcaaaaa tttcctgata gtcgtcaccg
      301 cgtttcgcgc actctttctc gtaggtgctc agtccggctt ctatcagcat caccgcttcc
      361 tgaacttctt tcagaccatc gatggccata cgaccggagc ctatccagtc gcagttcccc
      421 caggcactgc gggcttcctg aaagctgaag cgcgcttttg aaggtaacgt caccacgcgg
      481 cgaacgatgg cctcttccag ccagcacaga aacatctggc tcgcctgacg ggatgcgacg
      541 aattttcgcc gccccataaa gtacgcccac gactcgttcg cactggcccg tgccgtggag
      601 tagctcatct gggcgtaatt ccgggaaagc tgctcatacg agacacccag cccggcagcg
      661 atataccgca gcagtgactg ctcaaacacg gagtagccgt tatccgtgtc ctgagccgtc
      721 tgcaggttca gtgagtcccc cggcatcagg tgcggcactt ttgcgcctcc cagacggacc
      781 ggtgctgcgg cgtaatacgc ggcaatttca ccaatccagc cggtcagcct ttcccgctgc
      841 tcctgactgt tcgcgcccag aataaaatcc atcgctgact gcgtatccag ctcactctca
      901 atggtggcgg catacatcgc cttcacaatg gcgctctgca gctgcgtgtt ctgcagcgtg
      961 tcgagcatct tcatctgctc catcacgctg taaaacacat ttgcaccgcg ggtctgcccg
     1021 tcctccacgg gttcaaagac gtgaatgaac gaagcgcgcc cgccgggtaa ctcgcggggt
     1081 atccatgtcc atttctgcgg catccagcca ggatacccgt cctcgctgac gtaatatccc
     1141 agcgccgcac cgctgtcatt aatctgcaca ccggcacggc agttccggct gtcgctggta
     1201 ttgttcgggt tgctgatgcg cttcgggctg accatccgga actgtgtccg gaacagtcgc
     1261 gagggacggg tatcccaggt ggcctgaacg aacagttcac cgttaaaggc gtgcatggcc
     1321 acaccttccc gaatcatcat ggtaaacgtg cgttttcgct caacgtcaat gcagcaacag
     1381 tcatcttcgg caaactcttt ccatgccgct tcaacctcgc gggaaaaggc acgggcttct
     1441 tcctccccga tgcccagata gcgccagctt gggcgatgac tgagccggaa aaaagacccg
     1501 acgatatgat cctgatgcaa ctggatggcg ttggcggcat agccgttatt gcgtaccaga
     1561 tcgtctgcgc gggcattgcc acgggtaaag ttgggcagca gggctgcatc cacactttca
     1621 cccggtgggt tccacgcccg caactgccca ccaaatccgc tgccaccgcc gtgataaccg
     1681 gcatattcac gcagcgatgt catgccgtcc ggccccagaa gggtgggaat ggtggacatt
     1741 ttcatacata aaatcctgc
//
LOCUS       DAZFDC010000318         1731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFDC010000318 DAZFDC010000000
VERSION     DAZFDC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.320"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..562
                     /gene="escR"
                     /locus_tag="ACHJ48_005042"
     CDS             <1..562
                     /gene="escR"
                     /locus_tag="ACHJ48_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO4710377.1"
                     /translation="TSFLKISIVLGILKNALGIQQVPPNMALTSVSLILTMFIMSPII
                     LQINDNISQEPINYTDSDFFQKVDEKILSPYRGFLEKNTEKDNVEFFERAAQKKLGNE
                     TILKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTI
                     SIPFKILLFILVGGWQKLFEFLLVVN"
     gene            562..831
                     /gene="escS"
                     /locus_tag="ACHJ48_005043"
     CDS             562..831
                     /gene="escS"
                     /locus_tag="ACHJ48_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO4710378.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            831..1607
                     /gene="escT"
                     /locus_tag="ACHJ48_005044"
     CDS             831..1607
                     /gene="escT"
                     /locus_tag="ACHJ48_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO4710379.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGIQSILDKLYLSYEILPLQAD
                     IAFNRALIDFLFSLWDSFIKLMLSFSVPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            1600..>1731
                     /locus_tag="ACHJ48_005045"
     CDS             1600..>1731
                     /locus_tag="ACHJ48_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO4710380.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     "
BASE COUNT          521 a          291 c          304 g          615 t
ORIGIN      
        1 tacttcattc ctgaaaattt ctattgtact ggggatattg aagaacgcat taggcattca
       61 acaggtacca ccaaacatgg cgctaacatc agtgtctttg atactgacaa tgtttattat
      121 gtctccgata atattacaga taaatgataa tatttctcag gaaccaatca attataccga
      181 ctctgatttt tttcaaaaag ttgatgagaa aatattatca ccatatcgcg gttttttaga
      241 aaaaaataca gagaaagaca atgtagagtt ttttgaacgt gcagctcaaa aaaaattggg
      301 taatgaaact atcttaaaaa aagactctct atttatactg ttaccggctt tcacgatggg
      361 gcagcttgaa gctgcattca agataggttt tttgctctat ttacccttta ttgcgataga
      421 tttgatcatt tccaatatct tattggcctt gggtatgatg atggtatcgc cagtaacaat
      481 ttcgattcct tttaagatct tattattcat tcttgttgga ggctggcaaa aattattcga
      541 atttctgttg gtggtgaatt gatggatact ggatattttg ttcaattatg tgtgcaaacg
      601 ttctggataa tatttatcct ctcattgcct acagtcatag cggcctctgt tatcggtatt
      661 attattagtc tggtccaggc tataacgcag ttacaggatc aaacattgcc ttttttgcta
      721 aaaataatag cagtgtttgc tacgcttgcc ctgacttatc actggatggg aacaacaatc
      781 atcaacttct cgtcgataat atttgaaatg attccgaagg tgaacggcta atgaatgaga
      841 taatgacggt catagtatca tcattttatt gtatactcag gccgctgggg atgtttatta
      901 tcctaccaat attttcaaca ggtgttttac tcagtaactt tattcgcaac tcaattatga
      961 ttgcgtttac gcttccgatc atagtagaga actatacatt ttcggaaaaa ttgccatctg
     1021 gaatctttca gttgacagga atagcgctaa aagaaataag tattggtttt ttcattgggt
     1081 tatcatttac tattcttttt tgggcaatag atgcggctgg acagattatt gatactctaa
     1141 gaggctcaac aatatcttca atttttaacc cgtccataag tgattcatct tctatcactg
     1201 gcgttatctt gtaccaattt atctctgtga tctttgttat tcatggtggg atacaaagca
     1261 tcctagataa gctatattta tcctacgaga tattaccatt acaagccgat attgcattca
     1321 atcgtgcttt aatagatttt ttgttttctc tatgggattc atttattaaa ctgatgttat
     1381 cattttcagt tcccatgatt atcggtatat tcttatgtga tatggggttt gggtttctta
     1441 acaaaacagc acctcagcta aacgtattca cattatcact gccagtaaaa agtttgatag
     1501 caatctttat attgctatta gttattcatg tttttcctga ttttattacg gcaaatattc
     1561 attctgacat aattgataga tcgttaccga gcatgattaa tgagtgaaaa aacagaaaag
     1621 cccacaccca aaaaactaag ggatctaaaa aagaagggcg atgtaacaaa aagtgaagag
     1681 gtaatggctg cagtgcagtc attaatctta ttttcatttt tttctttata t
//
LOCUS       DAZFDC010000319         1725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFDC010000319 DAZFDC010000000
VERSION     DAZFDC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.321"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..671)
                     /locus_tag="ACHJ48_005046"
     CDS             complement(<1..671)
                     /locus_tag="ACHJ48_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO4710381.1"
                     /translation="MNCLIFFKPKNYHLKSKLNYYPITQAAFLGETEVVSNLIKLGVD
                     LDARGDLGRTALNEAVSNGYFDIVKLLVESGADLTIKDEFGKTALELAEVHQNYKIVE
                     WLSHYRDHNPIPDFSVLINIYGERYFGGEIIDTDARTELGEGVLHIAVRKRRQLDVRC
                     FIQHGADINAKANNGFDFTPLHYSIGIGDFDMMKLLLKLGADIYLESEMGYSPMDLAI
                     LMGDK"
     gene            complement(811..>1725)
                     /locus_tag="ACHJ48_005047"
     CDS             complement(811..>1725)
                     /locus_tag="ACHJ48_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4710382.1"
                     /translation="RLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIDIKGGWNLYSYALNPVSWIDPLGLTQCDSEGCNNDIL
                     FTGGSGPDNKILNELGPRDGIDGLGSQNMKMYSGLLGGDILKPGILGGLTLGSVTQRP
                     SRTAEEEVQAQIEYLAYKKRCEQKPDSNLSRCAAAIFQKSRKEDCLRMRQEWDDKWWP
                     GKHADEIKNVKASLKNTLIDVKKKMHAN"
BASE COUNT          461 a          472 c          272 g          520 t
ORIGIN      
        1 ctcttatccc ccatcaaaat agccaaatcc ataggggagt atcccatctc actctccaaa
       61 taaatatcag cacctaattt caacaaaagc ttcatcatat caaaatcccc tatccctatc
      121 gaataatgca aaggagtgaa gtcaaagcca ttatttgcct tagcatttat atcggcacca
      181 tgttgaataa aacatctgac atctaattgt cttcttttcc taacagctat atgaagaacc
      241 ccctcgccaa gttctgtcct tgcatctgta tctataatct ctccaccaaa atatcgctca
      301 ccgtagatat taataagtac agaaaaatcg ggtatgggat tatgatctct ataatgtgat
      361 agccattcaa ctattttata attctggtgt acctcagcca attctaatgc cgttttccca
      421 aattcatcct ttatagtaag atcggctcct gattctacta aaagcttaac aatatcaaaa
      481 tacccattac ttacggcctc attaagtgcg gtacgcccca aatccccacg agcatccaga
      541 tccacaccca atttaattaa attacttaca acctcagtct cacctaaaaa tgcagcctga
      601 gttataggat aataatttaa ttttgactta aggtggtaat ttttcggctt aaaaaaaatc
      661 aggcaattca tcactatagc ctccccccca ttggcttcct ccataaatcg gcagcatatt
      721 aaagataaga aaatatatta tagcattttc accaatcaat gggcataatt aacatcatta
      781 tattttaagg atacctatct cggaatgcca ctagttggca tgcatctttt ttttaacatc
      841 aataagtgtg tttttcaacg atgctttcac attttttatt tcatccgcat gtttgccagg
      901 ccaccattta tcatcccatt cttgtctcat tcttaaacaa tcttcttttc tgctcttttg
      961 aaagatcgcg gcagcacaac gacttaaatt actatcaggt ttttgctcac aacgcttctt
     1021 atatgctaaa tattctattt gagcttgcac ctcctcctca gccgttcgcg atggtcgttg
     1081 tgtcacacta ccaagcgtca aaccgcccag aatccccggt tttaaaatat cgccccctaa
     1141 tagcccacta tacattttca tgttttgtga acccaaaccg tcaatgccat ctctcggacc
     1201 taattcatta agtattttat tatcgggacc acttcctcct gtaaataata tatcattatt
     1261 acacccttcg gaatcgcact gcgtcaatcc taatgggtca atccaactta ccggattaag
     1321 cgcataagaa tataaattcc atcctccttt tatatctatt ggatcctggg tgatatacct
     1381 cccctgtagc gggtcatagt accggtgacg gttgtaatac agccccgact cctcatcata
     1441 ctgctgtccc ggcagacggt acggctgttg caggtgctgc gcgctggttt cgcccagcag
     1501 gtttccccac tcgtcatact ccccctgcca cgccgtttcc ccttccgggc tgatgagcgc
     1561 ctgcggcagt ccccggtggt cgcagtggta aagatgaagt ttccgctccg ggatatattc
     1621 cgcctccagc tgcgcggcca tctgttccgc cgtcagcccg cactgcgcaa gccactgctg
     1681 gctttcttca ctcacgctgc cctgccgcag ttcccgctcc agcct
//
LOCUS       DAZFDC010000320         1710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFDC010000320 DAZFDC010000000
VERSION     DAZFDC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.322"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(109..552)
                     /locus_tag="ACHJ48_005048"
     CDS             complement(109..552)
                     /locus_tag="ACHJ48_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO4710383.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLDLAYVGE"
     gene            709..1638
                     /gene="metA"
                     /locus_tag="ACHJ48_005049"
     CDS             709..1638
                     /gene="metA"
                     /locus_tag="ACHJ48_005049"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO4710384.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          416 a          469 c          386 g          439 t
ORIGIN      
        1 aagcgaaagg ccatcctgac ggatggcctt tttgcgttgg tgcaaaaaaa tgccggatgc
       61 gacgctggcg cgttttatcc agcttacgca ggcacgatag ggggcagctt attcccccac
      121 atacgccaga tccagcaacg gatacggttt ccccaaatcg tccacctcag agcgtcccgt
      181 aaccttaaaa cccaccttct tatagaaccc aaccgcctgc tcattttgct cattaacatt
      241 ggttgtcagt tccggggcca ttgagagcgc atgcttcacc agcatccggc ccacgccgca
      301 gccgcgcaca tcaggatcga taaacagcgc atccatatgc tgcccactta acaacataaa
      361 cccaaccggc tgatcccgtt cattaaccgc gacccacaac ggtgcttccg gcagaaagga
      421 acgaaccagc tcttccagct cggcccgata ctctgctgat aaaaaatcgt gggtggcatc
      481 gacagatcga caccaaatcg caacgagttc ctccccttcc tcatgccgtg agcggcgaat
      541 actaataacc attttctctc cttttagtca ttcttatatt ctaacgtagt cttttccttg
      601 aaactttctc accttcaaca tgcaggctcg acattggcaa attttctggt tatcttcagc
      661 tatctggatg tctaaacgta taagcgtatg tagtgaggta atcaggttat gccgattcgt
      721 gtgccggacg agctacccgc cgtcaatttc ttgcgtgaag aaaacgtctt tgtgatgaca
      781 acttctcgtg cgtctggtca ggaaattcgt ccacttaagg ttctgatcct taacctgatg
      841 ccgaagaaga ttgaaactga aaatcagttt ctgcgcctgc tttcaaactc acctttgcag
      901 gtcgatattc agctgttgcg catcgattcc cgtgaatcgc gtaacacgcc cgcagagcat
      961 ctgaacaact tctactgtaa ctttgaagat attcaggaac agaactttga cggtctgatt
     1021 gtaaccggtg cgccgctggg cctggtggag tttaatgatg tcgcttactg gccgcagatc
     1081 aaacaggtgc tggagtggtc gaaagatcac gtcacctcga cgctgtttgt ctgctgggcg
     1141 gtacaggcag cgctcaatat cctctacggc attcctaagc aaactcgcac cgacaaactc
     1201 tctggcgttt acgagcatca tattctccat cctcatgcgc ttctgacgcg tggctttgat
     1261 gattcattct tggcaccgca ttcgcgctat gctgactttc cggcagcgtt gattcgtgat
     1321 tacaccgatc tggaaattct ggcagagacg gaagaagggg atgcatatct gtttgccagt
     1381 aaagataagc gcattgcctt tgtgacgggc catcccgaat atgatgcgca aacgctggcg
     1441 caggaatatt tccgcgatgt ggaagccgga ctagacccgg aagtaccgta taactatttc
     1501 ccgcacaatg atccgcaaaa caaaccgcga gcgagctggc gtagtcacgg taatttgctg
     1561 tttaccaact ggctcaacta ttacgtctac cagatcacgc catacgatct acggcacatg
     1621 aatccaacgc tggattaatc ttctgtgata gtcgatcgtt aagcgattca gcaccttacc
     1681 tcaggcacct tcgggtgcct tttttatttc
//
LOCUS       DAZFDC010000321         1676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFDC010000321 DAZFDC010000000
VERSION     DAZFDC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.323"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..519)
                     /locus_tag="ACHJ48_005050"
                     /pseudo
     CDS             complement(1..519)
                     /locus_tag="ACHJ48_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
     gene            complement(632..901)
                     /locus_tag="ACHJ48_005051"
     CDS             complement(632..901)
                     /locus_tag="ACHJ48_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4710385.1"
                     /translation="MNILKKLMQRLCGHGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(903..>1676)
                     /locus_tag="ACHJ48_005052"
     CDS             complement(903..>1676)
                     /locus_tag="ACHJ48_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4710386.1"
                     /translation="SAAAQKASESSQSAAEAELSRKTAESAAGNAARDATTATEKARE
                     SAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGDTGPAGA
                     TGERGPAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGET
                     GAAGPVGATGPQGPKGDPGETQIRFRLGPASIIETNSHGWFPGTDGALITGLTFLAPK
                     DTTRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          343 a          517 c          362 g          454 t
ORIGIN      
        1 gacttataat cattgatgcc gttattggtt ctcgggtcag tggatgatat gagccttcac
       61 tagctaaacg agaaaatgca tcaaaatcaa ataagcagca tactgccgaa ctatctgaat
      121 ttttgacaaa cactccttct tcgggtctct ctagtgtaat tggacactga atagactctg
      181 gcgggcactg gaaatttcct gaactaacag gaaacttaca ttgagatatt ttgtcctgaa
      241 tactctcttg cgattgtgct tcacctgagt ccgaaagcat actgagcatc ctatcgcgaa
      301 gagcttcagg gccgttatta aacccataaa ggaataactc agtaagcaat cccccactac
      361 tgccgctgga tagaaaacgc ccatcggttt cattaaatac aatgctgaca gtctcattac
      421 ctaatgtaaa ctcccctcga ccgctagatg ctacttgagc tcgaaggaca ttcaatccat
      481 taaaaatgag cttgatctaa tctccgaatt taacggcatt ataaaactcc ctattttaat
      541 ttaaactcca gacttaaata gctttaacaa acatctgcct tacatgacaa caaaaaccgg
      601 agccggactc cggttttgtg aagctgtcgg gttacttcat cccgccaata ttttcccacg
      661 tcccgtcagc acgcaggatt tgcagcggtc ttaccacgca ctgtatctgc tttttatccg
      721 catccagtat caccacctgc gtgattaccc tgtcctgctc cggaataata ccattctcat
      781 cggactccag gatgtctgcc ggccccagac gcagttgtgc tgtaagtaac tccccgtctt
      841 cacggtcatc atgctttccg tgcccgcaca gacgctgcat aagttttttt agtatgttca
      901 tgtcattctc ctgttctgcc tgtatcactg cccacttcat caagcccctt aacatcctgc
      961 cacggcccgt caccaaacct gacctgcaaa tgctgaaaaa aaccctgaac ccgtgtggta
     1021 tctttggggg caagaaaggt cagtccggtg atgagcgcac catctgtacc cgggaaccag
     1081 ccatggctgt ttgtctcaat aatgctcgcc ggccccagac gaaaacggat ttgtgtctcc
     1141 cccgggtcgc ccttcggtcc ctgaggtccg gttgccccca ccgggccagc cgcacctgtt
     1201 tctcctttcg gtccctgtgg gcctgccggg cctgctgccc cggtgtctcc ctttggaccc
     1261 tgtggacctg catttcccgt cagaccggtc tctccccgct ctcccctgtc acctttcggc
     1321 ccctgcgggc ctgccggacc agcatcacct gccggtcccc gttcgccggt tgcccctgcc
     1381 gggccggtgt cgccacgctc tcctttatct cccttcggcc cctgaggacc cgcgggaccc
     1441 ggttccccct ttggcccggg aggtcccacc acggtgggga ttcggtttac ggcttcttcc
     1501 gccgctatcc tgctttgttc cgctgactgt gcgctttctg ctgactcccg ggctttttct
     1561 gttgcggtcg ttgcatccct ggctgcatta ccggctgcac tttctgccgt ctttcttgac
     1621 aattcagctt ctgctgcact ttgtgatgac tcactggctt tttgagcggc cgcaga
//
LOCUS       DAZFDC010000322         1674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFDC010000322 DAZFDC010000000
VERSION     DAZFDC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.324"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1151
                     /locus_tag="ACHJ48_005053"
                     /pseudo
     CDS             1..1151
                     /locus_tag="ACHJ48_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
     gene            1521..1674
                     /locus_tag="ACHJ48_005054"
                     /pseudo
     CDS             1521..1674
                     /locus_tag="ACHJ48_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          535 a          268 c          358 g          513 t
ORIGIN      
        1 aggcaacgcc tttgtcatta tgggagctgg ggaacaatta aaacgtatta aatatgatgt
       61 tgatgaaaat aacttaaagg tattcaacgt acactttgat aataatgaag tgttagttac
      121 tgatggtgag cctgacgtag tatgtttaag caagcaggtc tgggaaaatc ttctcattaa
      181 attaaaaccg gagatcaagg aaaatgtggc ttctgaagtt cataaatcag cgaataaagg
      241 tgagattgag caattagttg aatggtctaa aagaaatgaa caaacccttt tcgataatat
      301 aataaaaagt gattttcatg ttggttcact taagccaggt agtatgaatg gtgttatttt
      361 agaaatgcca ccaaatgtct gtatggaacc acgtaattca tatgaaaaca aaatagatga
      421 ggtttcatct ttgtcagagt cagaggaaca ccccatagat attcaaaaaa taacagatgc
      481 gtttgtgaag gagttcaagg ggatattatt tgataaaaat ggaagatctt cagagcttct
      541 gtttaatttt tatgaatgtt gctatacgtt tttaccaaga gcgcagcctc aagataaaat
      601 cgatagctat aattcagcac tgcaagcttt ttccatcttt tgttcatcta cgttgacaca
      661 taataatgta ggctttgatt tcaaattatt tccagaagtc aagctgtctg gagaacatct
      721 tgaaacggta ttcaaataca aaaatggcga tgatgtccgg gagatagcca aaattaacat
      781 tactctccaa aaagaagagg gtggcttata taatctacgt ggattggatt ttaagggatg
      841 cttcttttct ggacagaact tcagtaacta tgatattcaa tatgtgaact ggggaatgtc
      901 attgtttgat gttgatactc cgtgtatttt taatacgcct gctaaccatg agagttatga
      961 aaaatcatta aaacctgtaa gcgaaaacgg tttaaatgga gtcttatctg atcgtaataa
     1021 aaaaaataaa aatgatcacg ggtgtggcac cattcgatga tattttattt atggatgatg
     1081 actttgatga taactcccct gaggatgctc ccattgagaa tagtcctgtt gtgaatagtc
     1141 ctcttgtata aagttctaaa agcagttttc aatgataaat aaatggcaat ttttataaag
     1201 agataataac gtagattgaa acaatatgta ttattgattg agtaaacggg attattcttc
     1261 acactacatc cattttagtg tcactaccaa agaaaaaggg gctacgtttt catgtaaccc
     1321 cttgatttat ttggtgggct ggcagtgact gaattggtgc tgtaattggt tgaaatattt
     1381 catctattgt ctggttcaac tttccttagc tgcctgaaag ttttttgatc tcacccagca
     1441 ataacagcgt tacggtgcac cacgcctgac tgaagaactg cgtgctcagg gttaccgctt
     1501 caacgtaaaa ccgtggcagg aggtccggac tgaaaaaact gctcaggaca tcatcggcag
     1561 ggaaagcact gctagtctgt aaactgaacc gactggggcg cagtatgtgg catcttgtcg
     1621 tgctgttgga ggagctgtgc gaacgtggta ttaactttcg tgctctggcc caat
//
LOCUS       DAZFDC010000323         1657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFDC010000323 DAZFDC010000000
VERSION     DAZFDC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.325"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..99
                     /locus_tag="ACHJ48_005055"
     CDS             <1..99
                     /locus_tag="ACHJ48_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4710387.1"
                     /translation="GEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            345..>1657
                     /locus_tag="ACHJ48_005056"
     CDS             345..>1657
                     /locus_tag="ACHJ48_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO4710388.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFG"
BASE COUNT          394 a          424 c          537 g          302 t
ORIGIN      
        1 ggtgaaatgc tggttggctc ccggcgaatc tcccaggaca tcagtacccg tgatgaaggc
       61 ggtgacggga aggtggtggt tatcgggcgg caggcataaa agcgaaaaaa tcccgcagtg
      121 accgaaggct gcgggaacag aaaatgaaga ttaaccacag ggggttttgt ttttattggc
      181 ccgaaaaaac tgtaacgccc gggaatgata tctgccacgg gggcgtacag aaaatgtgaa
      241 gaaattcaga aattttattc cgtcatgaca caggcaccct ccggggtgcc tgtcgttttt
      301 ggggcataaa cagattcaga catcagacag gagaggggga cagagtgggt aaaggtggcg
      361 gcaaggcgca cacgccggtt gaggcaaagg acaatcttaa gtccacgcag atgatgagcg
      421 tgattgatgc catcggtgag ggaccggtgg aaggtccggt gaaggggctg cagagtattc
      481 tggtgaacaa aaccccgctg acggacacgg acggtaatcc tgtgatacat ggtgtgacag
      541 cggtctggcg cgccggggag caggagcaga caccgccgga aggttttgag tcatccggct
      601 ctgaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc accattacgt
      661 ccgcgaacat tgaccgcctg cgggttacct tcggggtgca gtcactggtg cagaccacct
      721 cacagggtga ccgtaacccg gcatccgtcc gcctgctgat tcagctgcag cgtaacggta
      781 actgggtgac ggaaaaggat gtcaccatta acggcaagac cacctcacag ttcctcgctt
      841 cggtgattct ggataatctg cctccccgcc cctttaacat ccggatggtc agggagacgg
      901 cggacagcac cacggaccag ctgcagaata agacgctctg gtcgtcatac accgaaatca
      961 tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg gatgcggagc
     1021 agtttggcgg tcagcagatg acggtgaact accatatccg cggtcgcatc attcaggtac
     1081 cgtcaaacta tgacccggaa aaacgcacgt acagcggcat ctgggacggc agcctgaaac
     1141 cggcatacag caacaacccg gcctggtgcc tgtgggacat gctgactcac ccgcgctacg
     1201 gcatgggaaa acgtctgggg gcggcagacg tggacaagtg ggcgctgtat gccattgcgc
     1261 agtactgcga ccagacggtg ccggatggtt tcggggggac cgagccgcgg atgaccttta
     1321 atgcgtacct gtcacaacag cgtaaggcat gggatgtgct cagtgatttc tgctcggcga
     1381 tgcgctgtat gccggtatgg aacggccaga cgctgacgtt cgttcaggac cgcccgtcgg
     1441 atgtggtgtg gccgtacacc aacagcgatg tggtggtgga tgataacggc gtggggttcc
     1501 gctacagctt cagcgccctg aaggaccgcc acacggcggt ggaggtgaat tacaccgacc
     1561 cgcagaacgg ctggcagacc tccacggaac tggtggaaga cccggaagcc atactgcgct
     1621 acgggcgcaa cctgctgaag atggatgcgt tcggctg
//
LOCUS       DAZFDC010000324         1650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFDC010000324 DAZFDC010000000
VERSION     DAZFDC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.326"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..648)
                     /gene="perB"
                     /locus_tag="ACHJ48_005057"
     CDS             complement(<1..648)
                     /gene="perB"
                     /locus_tag="ACHJ48_005057"
                     /EC_number="2.3.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine N-acetyltransferase"
                     /protein_id="HFO4710389.1"
                     /translation="MNLYGIFGAGSYGRETIPILNQQIKQECGSDYALVFVDDVLAGK
                     KVNGFEVLSTNCFLKAPYLKKYFNVAIANDKIRQRVSESILLHGVEPITIKHPNSVVY
                     DHTMIGSGAIISPFVTISTNTHIGRFFHANIYSYVAHDCQIGDYVTFAPGAKCNGYVV
                     IEDNAYIGSGAVIKQGVPNRPLIIGAGAIIGMGAVVTKSVPAGITVCGNPAREMKR"
     gene            complement(692..1623)
                     /locus_tag="ACHJ48_005058"
                     /pseudo
     CDS             complement(692..1623)
                     /locus_tag="ACHJ48_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          496 a          363 c          259 g          532 t
ORIGIN      
        1 tcttttcatt tctcttgctg gatttccgca cacagttata ccggcaggaa cacttttagt
       61 gacaacagcc cccataccta taatggctcc cgcgccaata ataagtgggc gattaggaac
      121 accctgctta attactgcac ccgagcctat atatgcattg tcttcaataa caacatatcc
      181 attacattta gccccaggag caaatgtaac atagtctcct atttgacaat catgtgcaac
      241 gtatgagtat atgtttgcat gaaaaaacct ccctatatga gtattagtag atattgtaac
      301 aaagggagaa ataatagcgc cactacctat catagtatga tcataaacaa cgctatttgg
      361 atgttttata gttattggtt caaccccgtg taataatatt gactcagaca ctctctgtcg
      421 tatcttatca ttagcaatag caacattaaa atactttttt aaataagggg cttttagaaa
      481 gcagttggtt gaaagcactt caaaaccatt aactttcttt cctgccaaaa catcatccac
      541 aaaaaccaga gcatagtcag aaccacattc ttgctttatt tgttgattta gaatgggtat
      601 tgtttctcta ccataacttc cagcaccaaa aataccatac aaattcattt tcccatctcc
      661 aaagaaatat cttatcaaaa ccaatgcaag attatgaaag cccgcacgct gcaatgcctg
      721 acgctaggaa gtttctgtcc atgactgctt ttcgcatctt acatgataat cctgccttga
      781 atacattatc attgtcagat attaatagcg atgtgccgca ttctagaaaa tcattcgaat
      841 gcaactcgcc ttcttatatt atagtcgatt tcattcatta ccacatcagg ctactgtgca
      901 acttattctc catgcgccag tgatttcgat ttactgtggc gaacttctct gcggttaaag
      961 cagcagatct aatataatat ttgatcgtca tttcggattc tttcttttgc tctgctatta
     1021 ttgagagaaa gtggactgcc attcataaat tctgcagccc tttccattca aacgtgaaat
     1081 caataagctc atcaggagca tctcaaacaa tatgaagaca gacatcgtct ctgccgtgcc
     1141 tcttttcact aattgcgtaa ctgtcatgtt ttggattatt taattctttc agcgtaaata
     1201 tctcctcaaa gactctatta agccgactct tatttccttt tacagcgaat aaataatcac
     1261 atctctgttt ttatatcttc tctgcaatat ctttctggca agccatcgca tcagttatga
     1321 ttatttttcc tttaataccc atcattttag aaagttcatg aataactgtg aacgcattag
     1381 gtttctcatc cgtcttgatc tatccgagga ccagactgtg cattgctgaa acgcactaat
     1441 gacatgaacc actcctctgc gacgactctt gtcataagtg tgccgaagtg ttttaccatc
     1501 aattgcgatg acgtcgttat catctggaaa atggcagaca tgtattcagt taacaaagca
     1561 ctcgtgaaat attgcaggac tgatgcagga tacaactttg gctataatat cgtaaacagg
     1621 gataataaaa atctattgtc tcaattattt
//
LOCUS       DAZFDC010000325         1643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFDC010000325 DAZFDC010000000
VERSION     DAZFDC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.327"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1525
                     /locus_tag="ACHJ48_005059"
     CDS             <1..1525
                     /locus_tag="ACHJ48_005059"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO4710390.1"
                     /translation="LSDEHLLSSHIIYSNIQKVTSSRGNPLISRVPPDFFDLIIIDEA
                     HHAPAQSWRDTLHYFSNAKILHVTGTPYRGDNQEIPGVKIHETPLSEVMRDKYVKWLR
                     KETVNAHDLFFYTPEQPGVKLSKEQVLMFKDKEWIEKSIALSKECSLDVINHSIKKLN
                     ELKELSPNVPHKILAIGCSISHAEDLLSWYKAKGMTSVIVHSEMDQNDLSDALMKIEH
                     HQCDVVISVNMLMEGYDHKYLTVLAIFRPYRSLNAFAQVVGRILRAIPESEITHFEID
                     NNGIVIFHEEIGLNVMWETFQKEVERARRASVREYTFTDRDYHERSNTLAGIESDSAY
                     VSDTDSYLDDVDFNAMFEQKRNEINNSVEQQIEKIKSSGVDLPEDALEALRQTLAEKA
                     VQKAAEIIDPELIEKRPHQARKLMRNILKTRTQDGVSNLLIDNGIGEKSSELYSIFNR
                     HVPYLKTTDPNDGILVRYVNLKLAKAYGPVDKRDNKTLLQSINDLDKILAEVERMIK"
BASE COUNT          598 a          269 c          306 g          470 t
ORIGIN      
        1 tttatctgat gaacatcttt taagcagcca cataatttat tcaaacatac aaaaagtaac
       61 atcttctcga ggcaacccac taataagtcg tgtaccccct gactttttcg atctcattat
      121 tattgatgag gctcatcatg cccccgcaca aagttggcgg gatactcttc attatttttc
      181 caatgctaag atattgcatg ttactggcac tccatataga ggcgacaatc aagaaatacc
      241 aggagtaaaa atacatgaaa ctcctttatc tgaagttatg cgggataaat atgtcaaatg
      301 gttaagaaaa gaaaccgtca atgcacatga cttattcttt tacacgccag aacagccagg
      361 agtaaagtta tctaaagaac aagttttgat gtttaaagat aaagaatgga tagagaaaag
      421 tatcgctctt tccaaagagt gctctttaga tgttattaat catagtataa aaaaacttaa
      481 tgaactaaaa gagctgtcac caaatgtccc acataaaatt ttggctatag gctgtagtat
      541 atctcatgct gaagatttac tttcatggta taaagccaaa ggaatgacat cagtaattgt
      601 tcatagtgaa atggatcaaa acgacctttc tgatgctcta atgaaaatag aacatcatca
      661 atgtgacgtc gttatatctg tcaacatgct catggaaggg tatgatcata aatatctaac
      721 agtacttgct atatttagac cataccgtag cctaaacgct tttgcacaag ttgttggacg
      781 cattttacga gcgattcctg aatcagaaat tacccacttt gaaatagata acaatggaat
      841 agtcatattc catgaggaaa taggtttaaa tgtcatgtgg gaaacatttc agaaagaagt
      901 ggagagagca cgcagagcaa gtgtaagaga atatacattt acagacaggg attatcatga
      961 aagatctaat acattagctg ggatagaaag tgatagtgct tacgtaagtg atacagactc
     1021 ttacctagat gatgttgact ttaatgctat gtttgagcag aagcgtaatg agatcaacaa
     1081 ttctgttgag caacaaatag aaaaaataaa atcgtctgga gtagatcttc ctgaagatgc
     1141 tcttgaagca ttacgccaaa cattagcaga aaaagctgtg caaaaagccg cagagataat
     1201 tgatcctgag ctaattgaaa aaaggcctca tcaagcgaga aagttaatga gaaacatctt
     1261 gaaaacccgc actcaggatg gtgtttctaa tttattgatt gataatggaa ttggtgaaaa
     1321 aagctccgaa ctgtatagta tttttaaccg acatgtacct tatttaaaga caaccgatcc
     1381 aaatgatggt atattggtta gatatgttaa cctaaaatta gccaaagcat atggtcctgt
     1441 tgataagcgg gataataaaa cccttcttca atcaataaat gatttggaca agattctcgc
     1501 agaagtagag aggatgatta aatgacaaac tattctcaaa tcatggaaga aattaataaa
     1561 ataatttctt tctgtatggt taaaggtgtt caacctcatg aattaattag tgcaatattc
     1621 gaagatgaat ataagcacat tga
//
LOCUS       DAZFDC010000326         1640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFDC010000326 DAZFDC010000000
VERSION     DAZFDC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.328"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..262)
                     /locus_tag="ACHJ48_005060"
                     /pseudo
     CDS             complement(1..262)
                     /locus_tag="ACHJ48_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            356..619
                     /locus_tag="ACHJ48_005061"
                     /pseudo
     CDS             356..619
                     /locus_tag="ACHJ48_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            659..779
                     /locus_tag="ACHJ48_005062"
                     /pseudo
     CDS             659..779
                     /locus_tag="ACHJ48_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            776..928
                     /locus_tag="ACHJ48_005063"
                     /pseudo
     CDS             776..928
                     /locus_tag="ACHJ48_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1035..>1640
                     /locus_tag="ACHJ48_005064"
     CDS             1035..>1640
                     /locus_tag="ACHJ48_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710391.1"
                     /translation="MIHPGSSLDKAINNTRVKNVSTDVKHGQIQERKRNFIYKKNDDI
                     SSRFKLYSSLVKQKNATEDVVLIGKMILDEVRSYRTIHNDRNIVSNSGNWKTSFLCNL
                     ARLLYSIFNGSNYFCSREGENNSSPSSTLLTIHQPEKQELLQQKSIKHLPTSNNIDGY
                     IKIRKTRGAEDQTTTITQSLIINELLNGVDRNTIPFQKISEL"
BASE COUNT          534 a          336 c          346 g          424 t
ORIGIN      
        1 gccactggcg taaaaatcct gctccagcag attttctgac acaggctggc tgtgtgcgcg
       61 gtaacagacc ggactgaaat tccaggaagc ttttgccctc agtccgtgac ggcgcaggct
      121 ttccgtcacg gtttttactt taaaatggta accctgggca cgcagttcat ccatcaggcg
      181 ttgtgcatga ccgggtaaaa gcaacgagga caacgctgtc gcagtgctga cggaactgcc
      241 gacgtgtact tatccttatc cgccagtgat ataagacggt aattcaccat ttggattgtc
      301 cgctccaccc aacatgttgt ttccttgagg ttctcacacc agaaaggaca tcagcatgct
      361 gagcagagag gacttttaca tgataaagca aatgcgccag caaggtgcgt atattgttga
      421 tattgcgact cagattggtt gctctgaacg aacggtcaga cgttacctga aatacccaga
      481 acctccggcc agaaaaacac gccacaaaat ggccgaaatg gactatatcg acatgcgtct
      541 ggctgagaat gtctggaacg gcgaagttat cctggcggaa atcaaagcga tgggttatac
      601 cggtggccgt tccatgttgc ttcgccagtt caggaagaca ccggagcagc gctttacgca
      661 ggaacacagt ctgttcttcc ggttgctgaa ccgtcgatat gaaaaagcga gcattgtgct
      721 gacgtccaat aaagggttcg ccgactgggg gagagatgtt cggtgataac gtgctggcaa
      781 aatattcacc caacgcattg agcgtaataa tctgacgcta cgcacccgca ttaagcggtt
      841 gactcgtaaa accatctgct tctcacgctc agttgagatc cacgaaaaag tgatcggagc
      901 gtttatcgaa aaacacatgt tctactaatt ggatgcacca ccgaatacat taatttatat
      961 aacaacatca aaatacattt taggttgtcg ctaatcaaaa attattaagt gttacataat
     1021 tagagggtat agatatgatt catcctggct cttctttaga taaagcaatt aacaatacaa
     1081 gagttaaaaa tgttagtact gatgttaaac atggccaaat tcaagagaga aaaaggaact
     1141 ttatctataa aaaaaatgat gatatctcat ccagatttaa actttacagt tcgctcgtaa
     1201 agcagaaaaa tgcgacagaa gatgttgttt taataggcaa aatgatttta gatgaagtta
     1261 gaagttacag aactatacat aatgatcgaa atatcgtaag taactcagga aactggaaaa
     1321 catctttttt atgtaatctt gctagactac tatatagcat atttaatggt agtaactatt
     1381 tttgttcccg agagggtgaa aataattcat cccccagttc tactctactt actatacatc
     1441 agcctgaaaa gcaggaacta ttacaacaaa agagtatcaa acatttacca acaagtaata
     1501 acatcgacgg atacattaaa ataagaaaaa caagaggcgc tgaagatcaa acaacaacta
     1561 tcactcaaag tttgataatt aatgagttgt taaatggagt tgatagaaat accatccctt
     1621 ttcagaaaat aagtgagctc
//
LOCUS       DAZFDC010000327         1617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFDC010000327 DAZFDC010000000
VERSION     DAZFDC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.329"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            55..177
                     /locus_tag="ACHJ48_005065"
     CDS             55..177
                     /locus_tag="ACHJ48_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO4710392.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            192..881
                     /locus_tag="ACHJ48_005066"
     CDS             192..881
                     /locus_tag="ACHJ48_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4710393.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDKTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            1065..>1617
                     /locus_tag="ACHJ48_005067"
     CDS             1065..>1617
                     /locus_tag="ACHJ48_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710394.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTG"
BASE COUNT          419 a          280 c          402 g          516 t
ORIGIN      
        1 ttacaggtag ttatggatga tgagtgaaac agatattgga gaaccgggga atgaatgatg
       61 tctgagtctt atatatcaga actccttcgc tgtcgctggg ggctcctgtg cttatgtcgt
      121 ttccccgatt cggttttgaa cgattaccga atgttgaaga attatgccaa aatatagaaa
      181 ggatttactg catgaatacc caatatttac agtatgttcg tgagcaactt atggcagcta
      241 ctgctgactt gaacggagca acgaaaggcc agctcgaagc ctggcaggag catgcacaat
      301 ttgatactgg tacatacaaa cgaaagaagc cgcgcattct ggatgtggta actggcaaga
      361 tgattacgct ggataagacg ccgacttccg gtaagcagtc gtacgcaaaa ggttcatcca
      421 ttgctttggt cagcccggtt gaattctcaa cctcttcatg gcgccgcgcg gttttgtctc
      481 tcgatgaaca tcagaaagca tggttgcttt ggtgttacag cgaaagcgtt cgatgggggc
      541 atcaggtcac cataacgcaa tgggcatgga gcgagtttaa agatttgtta agtaacagaa
      601 aaattgcagg taagacactg gatcgcctga agacgttaat ctggctggct gcacaggatg
      661 tgaagagcga acttgcaggg cgtgaggcct atgaatacca gacactggca tcattggtgg
      721 gagtgacaac aaaaaactgg tccgagacat ttactgaacg ctgggttgca atgaagcaca
      781 tttttctaca gcttgatagt gatgctttat tgcttgtgac gagaacacgt tcaaaacaaa
      841 aggcagcatt tttacagcaa aatattgcaa aactggatta aaagccatat acttcatgca
      901 aatttggtat gttgtaaaaa atgtataaac ccgctgccga gtgggttttt ttatgccctg
      961 agttgtactt gtacggtaaa catgctggct gctatgtaat agagtttttt tagcctgtaa
     1021 cctcttgacg gcattgaatt gcttttgtta tgagttgtaa gccaatgtta tcatcttgta
     1081 ttggggtggt tatgaaggat ggtgcgctgc tcaggagttc ttcacttttt attgcctaca
     1141 tgggatgcct tggatggggg agtgcttatt tctatggatg gggtacttct ttttactacg
     1201 gcttcccatg gtggattgta ggtgcaggtg ttgatgatgt tgccagaagt ttattttttg
     1261 cagttatcgt cattgctata tttcttatcg gttggggtat tggtgttgta ttctttttcg
     1321 cagtgaaaag aaaacattct atgcaagagc taaatgtatt tcgcctttat tttgctgtgg
     1381 aattattgtt tgtgccggca attattgagt tttctatatt gagacagaag attcaggtac
     1441 ctcttttgct actgtcagca gcgattgcgc tggcggttac aatttcgata agatcttatg
     1501 ggcgattttt atcggtatca tgcttctatg ataagccatt tataaaaaaa catttttttg
     1561 agattgtgat gattgctttt gtggcatatt tctggctttt ttcatttctg acaggat
//
LOCUS       DAZFDC010000328         1608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFDC010000328 DAZFDC010000000
VERSION     DAZFDC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.330"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            285..1262
                     /locus_tag="ACHJ48_005068"
                     /pseudo
     CDS             285..1262
                     /locus_tag="ACHJ48_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          460 a          374 c          378 g          396 t
ORIGIN      
        1 agatgctttt tacaatgaag aattattgtt aaaaaacgct ttgttgaatc aaccgaactg
       61 ttgctgagtt gcaggatcac atcacgtatt ttcctgaaaa cgcaggctgc ttagtagcaa
      121 agcagaagtt caaaatcacc aactaaatag ctgcgccgaa tagtagatca atgaaaggga
      181 actcagcccg gattgtgcga tctgatcaat cgccaaaaat ccccccctaa ccggactgag
      241 cgatgccgat catagcacca attccccgcg acgaatgacg cctgatgcaa aaagcaatct
      301 ataaaactca cgataaaaat tatgcccgca ggctgaccgc catgctgatg ctgcaccgga
      361 gcgctcgcgt cagtgacgtt gccagaacgc tctgctgcgc ccgttcctcc gtcaggcgct
      421 ggattaactg gttcacgctg tcgagtgttg aaggtctgaa atcattaccc gcaggacgtt
      481 cccgccgctg gccgtttgag catatctgca cattgtttcg tgaactgata aaacactctc
      541 ccggatattt tggctatcag cgttcacgct ggagtacaga actgctggca ataaaaatca
      601 acgagataac cgagtgccag ttacatgccg gaaccgttcg ccgctggttg ccattagtcg
      661 ggcttgtgtg gcgcagggct gcgccaaccc tgcgtacccg taacccgcat aaagcagcaa
      721 ttcacaaagc actggacgaa tgtagtgcgg agcatccggt cttttatgaa gatgaagtgt
      781 atatctatct taatcccaaa accggtgcgg actggcaact tcgcagacag caaaaacatg
      841 tggtaacacc ggggcagaat gaaaaatact atctggctgg tgctttacat agtggaacgg
      901 gcaaagtcag ctatgtgggt ggcaacagca aaagctcagc cttatttatc agtctgttga
      961 aacatctgaa attgacatac cgacgggata aacctatcac gctcatagtg gacaactaca
     1021 tcatccataa aagtcgggaa acacagcgct ggctgaaaga aaatccgaag ttcagagtga
     1081 tttatcagcc agtttactcg ccgtgggtga atcatgttga gcggttatag caggcgcttc
     1141 acgatacgat aacgcgcaat catcagtgcc gttcaatgtg gtaactgctt aaaaatgttc
     1201 attattttat ggaaaccgtg agcccattcc ccggtggtaa atatgggctg ttaaaagtgt
     1261 agcggtattc ggcgcagcta tttaggatgg tcaggtcgca gaagttctgt gccactacag
     1321 tgcgtgtact aaacaaaatg acgagggcac gaatgtcata atacgtgcgt attacctgac
     1381 agtagctacc aactgagggg gcttattcca aacctgattt attcaacaaa gctggactta
     1441 tccttactct gaattatttt ccagacatat gcatcatgta ctggctcgct tggttcagga
     1501 aatacaggag caaatctaca gctattagat ttattcctat ttgtatgcaa agtcgcattg
     1561 cataatgcgg agctatgtta cagatacgcc tgacgtgcgt agtaaaat
//
LOCUS       DAZFDC010000329         1596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFDC010000329 DAZFDC010000000
VERSION     DAZFDC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.331"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1596
                     /locus_tag="ACHJ48_005069"
     CDS             <1..>1596
                     /locus_tag="ACHJ48_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710395.1"
                     /translation="RYEDLYVKLMHYSVFTDKTHQIIKQKYFNDIVPMILALDIRNTY
                     RPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFDG
                     VYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLSNEDKEKIISDLDKVQVACYYL
                     TILLAFERRTSLTSTLATLYKMLISEREVSEYECQLLYLTNPIDVMNILNKYIYYFPN
                     ENSPFYTLKIDSALSWDAIDAIRDYSISDIYLYPEQKTINCVVEIENIVFGGYIYTLN
                     NGVTLQNIENSLKDSSCHYVLNGYTEFVNCLRQLTSGKTESVHRTINKLNYEKLPFGF
                     IIAAFAILKIAFKIKFSKNHVNIRALLNDINYFMTYQGESINLISLDHEYPESCLQND
                     TNTYLLGRVIFLYNSMIYKFINCQEHETNNIHSAMINNLLQEVDIALGKINDIIDSRN
                     ISAPHELANILTREKILTTREKKGNLISLFDGFTLFHCVGMITFLIHYLRTPEEKVEN
                     IFMLYGADKNNKLRRRLIYDALGI"
BASE COUNT          597 a          281 c          240 g          478 t
ORIGIN      
        1 agatatgaag atctctatgt taagctaatg cactattctg tttttacaga caaaacacat
       61 caaataataa aacaaaaata ttttaatgac attgtaccaa tgattctcgc actcgacatc
      121 aggaacacat atcgcccgga taatgagatg gcattttact atcatattca ttcttttctc
      181 actcagatac cggataatga ggatgatata tatcatgctg caaggacata tctgcgaaat
      241 tacgttaagt tatgtttatc cggatacacg ccagcgaatg cgcatttcaa agatatcttt
      301 gatggcgtat atgaattcat tcgtaatatt cgcaaaaaca gtacaccagg aaaaacaaaa
      361 cttatcgcaa ctatcaacac atgcaaagaa acctgtaaac atctgcttta tttaagtaat
      421 gaagacaagg aaaaaataat ttctgactta gataaagttc aggttgcatg ttattatctc
      481 actatattac tggctttcga aagacgaact tcattaacaa gcaccctggc aactttatat
      541 aaaatgctga taagcgaaag agaagtttca gaatatgaat gccagttatt atatttaacc
      601 aacccaatag atgtaatgaa tatactgaac aaatacatat attactttcc taatgagaac
      661 tcaccatttt atacactgaa aattgacagt gcattatcgt gggatgccat tgacgcaata
      721 cgagactata gtatttctga tatttatctt tatcctgaac aaaaaacaat aaattgtgtc
      781 gttgagattg aaaacattgt ctttggcggt tacatttata cattgaacaa cggcgtcaca
      841 ttacaaaaca tagaaaactc tttaaaagat tcttcatgcc attatgtctt aaatggctat
      901 acagaatttg ttaactgttt gagacaactt acttcaggaa agactgaaag tgttcatcgc
      961 accatcaata aactgaacta tgagaaatta ccttttggat ttatcattgc cgcgtttgct
     1021 atactaaaga tagcatttaa aataaaattc agtaaaaatc atgtaaatat ccgagcatta
     1081 ttaaatgaca tcaattattt tatgacttat cagggcgagt ccattaacct tatttcactg
     1141 gatcacgaat acccagagtc ctgtcttcaa aatgacacaa acacatattt attaggaaga
     1201 gtaatatttc tgtataactc aatgatttat aagttcataa actgtcagga acatgaaacc
     1261 aataacattc actcagctat gataaataac ctattacagg aagttgatat agcccttggt
     1321 aaaataaatg acattataga cagcagaaac atatcagccc cccatgaact ggcaaatatt
     1381 cttacccgcg aaaaaatact tacaacacgg gaaaaaaaag gaaacctgat aagcctgttt
     1441 gatggattca ctttattcca ttgtgttgga atgataacct ttcttatcca ttatctcaga
     1501 acacctgaag aaaaagttga aaatatattt atgttatatg gtgcagataa aaacaataaa
     1561 ctacgcagaa gactgattta tgacgcacta ggaata
//
LOCUS       DAZFDC010000330         1550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFDC010000330 DAZFDC010000000
VERSION     DAZFDC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.332"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..520
                     /gene="wzx"
                     /locus_tag="ACHJ48_005070"
     CDS             <1..520
                     /gene="wzx"
                     /locus_tag="ACHJ48_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFO4710396.1"
                     /translation="VFSITQRLFQISTVPLTIYNIPLWAAYADAHARNDTQFIKKTLR
                     TSLKIVGISSFLLAFILVVFGSEVVNIWTEGKIQVPRTFIIAYALWSVIDAFSNTFAS
                     FLNGLNIVKQQMLAVVTLILIAIPAKYIIVSHFGLTVMLYCFIFIYIVNYFIWYKCSF
                     KKHIDRQLNIRG"
     gene            523..>1550
                     /gene="rfbE"
                     /locus_tag="ACHJ48_005071"
                     /gene_synonym="perA"
     CDS             523..>1550
                     /gene="rfbE"
                     /locus_tag="ACHJ48_005071"
                     /gene_synonym="perA"
                     /EC_number="2.6.1.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-perosamine synthase RfbE/PerA"
                     /protein_id="HFO4710397.1"
                     /translation="MKYIPVYQPSLTGKEKEYVNECLDSTWISSKGNYIQKFENKFAE
                     QNHVQYATTVSNGTVALHLALLALGISEGDEVIVPTLTYIASVNAIKYTGATPIFVDS
                     DNETWQMSVSDIEQKITNKTKAIMCVHLYGHPCDMEQIVELAKSRNLFVIEDCAEAFG
                     SKYKGKYVGTFGDISTFSFFGNKTITTGEGGMVVTNDKTLYDRCLHFKGQGLAVHRQY
                     WHDVIGYNYRMTNICAAIGLAQLEQADDFISRKREIADIYKKNINSLVQVHKESKDVF
                     HTYWMVSILTRTAEEREELRNHLADKLIETRPVFYPVHTMPMYSEKYQKHPIAEDLGW
                     RGINLPSFP"
BASE COUNT          532 a          239 c          290 g          489 t
ORIGIN      
        1 tgtttttagc attacacaga gattatttca aatatctacg gtccctctta cgatttataa
       61 catcccgtta tgggctgctt atgcagatgc tcatgcacgc aatgatactc aatttataaa
      121 aaagacgctc agaacatcat tgaaaatagt gggtatttca tcattcttat tggccttcat
      181 attagtagtg ttcggtagtg aagtcgttaa tatttggaca gaaggaaaga ttcaggtacc
      241 tcgaacattc ataatagctt atgctttatg gtctgttatt gatgcttttt cgaatacatt
      301 tgcaagcttt ttaaatggtt tgaacatagt taaacaacaa atgcttgctg ttgtaacatt
      361 gatattgatc gcaattccag caaaatacat catagttagc cattttgggt taactgttat
      421 gttgtactgc ttcattttta tatatattgt aaattacttt atatggtata aatgtagttt
      481 taaaaaacat atcgatagac agttaaatat aagaggatga aaatgaaata tataccagtt
      541 taccaaccgt cattgacagg aaaagaaaaa gaatatgtaa atgaatgtct ggactcaacg
      601 tggatttcat caaaaggaaa ctatattcag aagtttgaaa ataaatttgc ggaacaaaac
      661 catgtgcaat atgcaactac tgtaagtaat ggaacggttg ctcttcattt agctttgtta
      721 gcgttaggta tatcggaagg agatgaagtt attgttccaa cactgacata tatagcatca
      781 gttaatgcta taaaatacac aggagccacc cccattttcg ttgattcaga taatgaaact
      841 tggcaaatgt ctgttagtga catagaacaa aaaatcacta ataaaactaa agctattatg
      901 tgtgtccatt tatacggaca tccatgtgat atggaacaaa ttgtagaact ggccaaaagt
      961 agaaatttgt ttgtaattga agattgcgct gaagcctttg gttctaaata taaaggtaaa
     1021 tatgtgggaa catttggaga tatttctact tttagctttt ttggaaataa aactattact
     1081 acaggtgaag gtggaatggt tgtcacgaat gacaaaacac tttatgaccg ttgtttacat
     1141 tttaaaggcc aaggattagc tgtacatagg caatattggc atgacgttat aggctacaat
     1201 tataggatga caaatatctg cgctgctata ggattagccc agttagaaca agctgatgat
     1261 tttatatcac gaaaacgtga aattgctgat atttataaaa aaaatatcaa cagtcttgta
     1321 caagtccaca aggaaagtaa agatgttttt cacacttatt ggatggtctc aattctaact
     1381 aggaccgcag aggaaagaga ggaattaagg aatcaccttg cagataaact catcgaaaca
     1441 aggccagttt tttaccctgt ccacacgatg ccaatgtact cggaaaaata tcaaaagcac
     1501 cctatagctg aggatcttgg ttggcgtgga attaatttac ctagtttccc
//
LOCUS       DAZFDC010000331         1548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFDC010000331 DAZFDC010000000
VERSION     DAZFDC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.333"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            297..458
                     /locus_tag="ACHJ48_005072"
                     /pseudo
     CDS             297..458
                     /locus_tag="ACHJ48_005072"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            442..507
                     /locus_tag="ACHJ48_005073"
     CDS             442..507
                     /locus_tag="ACHJ48_005073"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO4710398.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            623..880
                     /locus_tag="ACHJ48_005074"
     CDS             623..880
                     /locus_tag="ACHJ48_005074"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4710399.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ACHJ48_005075"
     CDS             complement(927..1496)
                     /gene="nleF"
                     /locus_tag="ACHJ48_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO4710400.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT          472 a          355 c          300 g          421 t
ORIGIN      
        1 aaaaacaact ttttatcttt ttgtttcgct caaattagta taaaaagccg aacaacaaac
       61 aataaaaaac cattaacatc aatagattat gatggcttca atctaaataa taggctatat
      121 aatactgcaa gacacaacac atgctgtcac tatgagtcaa ctatttagat gatattagtg
      181 acctgtttca gagcattagc gcaaggcgat tctttgtctt cttgcactaa ttttttatca
      241 taaaaatgtt cctagcactg ggcatcaata tcgcaggtca gaaagagctc ctggggatgc
      301 ggctggccga aaatgaaggg gcgaatttct ggttcaatgt gctgactgaa ctgaaaaacc
      361 gcggtctgaa cgatatcctc atcgcctgtg tgtatggcct gaaagaattc ccggaggccc
      421 gcatccagtt atgcatcgtg catatggtgc gcaacagcat gcgcttcgtg tcatggaagg
      481 aatacaaagc cgtcactcgc gacctgaaag cgattagcct cccacagaag aggcaggcca
      541 gcaggcactg gaagcgtttg ctgcggcctg ggactgccgc tatccgcaga taagccggtg
      601 ctagctgtca aactggacta acttggcgac gtttttcgct tatccggcag atatccgcaa
      661 agtgatctac acaacgaacg ccatcgagtc gctgaacagt gtgatccggc atgccatcag
      721 gaaacgcaag gtgttcccga cggacgagtc agtaaaaaaa gtggtgtggc tggcaatcca
      781 ggccgcgtca cagaaatgga caatgccgtt aagggactgg cgaatggcaa tgagccgctt
      841 tattatcgag tttggtgacc gtcttgacgg tcacttttaa gaaaaggcat ttacacagaa
      901 tcctaaacag gctcacaggc ctctaatcat ccacattgta aagatccttt gttgtaagta
      961 agatctggta ccctaataat atcaaaagga ttaaaatcat cactgcatcc ttccacgagg
     1021 catttcattg ctcgtagatc accatatagc tctgaagagt gatgcggaca tagcagatta
     1081 taaactttac atttcgaatc ttctttgcct tgtctacttt cctcaatttt tactttcaat
     1141 gaatgcagaa aaaaaagatc cctgatataa ctatccctgt cctctatcgt cattcccgcc
     1201 ctcagcgctc cgacaacctc atcagtattt tcatttctca cctcaaatac actatttctc
     1261 ttatccatca gcccacttac aaaagcactt agctcttgaa aaatttcctc aagctcatta
     1321 ttatttctgg cacatgaata tatagagacc ataacatcaa gtttatcttg taattcagga
     1381 gaaagaaagt gattatgatt aagctcactt actgattccg gagtcgaagg gttacctctt
     1441 cctgatacat acatccatga ataaagattt gctgaagaac cacttgttgg taacatatca
     1501 aaaccccctt aacaaataaa tccataaata ttattgcaac taatatat
//
LOCUS       DAZFDC010000332         1543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFDC010000332 DAZFDC010000000
VERSION     DAZFDC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.334"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1543
                     /locus_tag="ACHJ48_005076"
     CDS             <1..>1543
                     /locus_tag="ACHJ48_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4710401.1"
                     /translation="QATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRRVALIRQQAAE
                     QNKVYQSMLVMNGQYTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKT
                     ERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKPKS
                     RGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELKTLTEMQKQELL
                     RNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARERMRELQQIRD
                     SFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQEAFYAESDAQRADWQKG
                     LQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWASSVLQEISK
                     VLMNAAIVNGIKTAANSMSGAGGFLGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVD
                     TPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQ
                     NDGTNG"
BASE COUNT          397 a          348 c          516 g          282 t
ORIGIN      
        1 ctcaggcaac agagcagctt gccgttgagc agtcccgtct ggcacagatg caggaaaaag
       61 cgcagtccat tcaggatgtg cttgccgggc tggaagaccg tcgtgtggcg ttaattcgtc
      121 agcaggcggc agagcagaat aaggtgtacc agtccatgct ggttatgaac ggtcagtata
      181 cggaattcaa ccgtctgctg gggctgggga atgaactgct tcagcagcgg cagggactgg
      241 tgaatgtgcc gttacggctg ccacaggcca ctctggatga taaacagcag agtgccctga
      301 caaaaacaga gcgtgagctg gccctgtcca gactgaaagg ggaagaaaaa gagcgcgtcc
      361 gactggggta tgcggcggat gacctcggtt ttgtgggtga tccgtatcag gaggcgagac
      421 aacgttatat cagtaatgcc ctggaagcct ggcgcaataa cgaggcgaat aaacccaaat
      481 cccggggtgg aaaatcagag acggaaaaag cggaagacag tttttcccgg ctgctgaagc
      541 agcagaaaga gcaactggca ctggtggggc agaatacaga gctggcgaag ctgaaatacc
      601 agactgcgca gggcgaactg aaaaccctga cggagatgca gaagcaggag ctgctgcgta
      661 atgcggccct gattgaccag caaaaaatcc gggaacagtt gcgatcccgg gaagagacac
      721 tgaagaatga gaatgcggct gcgcgtgcgt cgaatgatgc tgaactgctg gggtacgggc
      781 agggggaacg agccagagaa cgcatgcggg agttgcagca gatccgcgac agcttccgcc
      841 agaaggatgc ggaccttcag tctcagtatc agaccgggga tatcagtgag gatttttaca
      901 gacaggctct ggcgcagaac gcgcagtatc tgagtgaacg tctgaaagag caggaagcct
      961 tttatgccga atcggatgcg cagcgtgcgg actggcagaa agggctgcag gagggattca
     1021 gtaactgggt ggataatgcg tccgattacg cctcacaggc agcacagctt gcgacggagg
     1081 gtatctcagg gatggtgaat aacatcacgg agatgctgaa cggaaataaa gtggaatggc
     1141 gcagctgggc ctcatcagtg ctgcaggaaa tatcaaaagt tcttatgaat gccgcgattg
     1201 tcaacggaat taagacggcg gcaaacagta tgtccggtgc gggaggattt ctcggcagca
     1261 ttggtgactg gttgggcggg gcggtggcca atgcaaaagg cggcgtgtat acctcggcaa
     1321 acctgagtgc gtacagcaac agcattgtgg atacgcccac gtactttgcc tttgcaaaag
     1381 gggcgggact gatgggggag gccggtcctg aagccattat gcccctgacc cgggcggcgg
     1441 atggctcgct gggtgtgcga gcggtgggca gtatgaacgg cagtgcgggt ctggtgtatt
     1501 ccccggtcta ccatatcgcc attcagaatg acgggactaa tgg
//
LOCUS       DAZFDC010000333         1525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFDC010000333 DAZFDC010000000
VERSION     DAZFDC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.335"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..394)
                     /locus_tag="ACHJ48_005077"
                     /pseudo
     CDS             complement(1..394)
                     /locus_tag="ACHJ48_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
     gene            652..1152
                     /locus_tag="ACHJ48_005078"
     CDS             652..1152
                     /locus_tag="ACHJ48_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO4710402.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIWINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNQLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
     gene            1182..1448
                     /locus_tag="ACHJ48_005079"
     CDS             1182..1448
                     /locus_tag="ACHJ48_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO4710403.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEH
                     PEYISSAELEEKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
BASE COUNT          523 a          275 c          230 g          497 t
ORIGIN      
        1 ctattttcaa taatgcctgg caatgaatac tgccaaactt cattttttct tttacagcaa
       61 atttgagacc taaaacttct atttgctgat tcaatgttaa cgttctatct tcaggttctt
      121 gtgctgttaa ttctctgatt gttacaacca gatcagggtc ggtagtgatt ataatctcaa
      181 aactacctgt aagctcgcca taaggaacga ctattgtatt ttctggtaac tcagaaaaag
      241 catcacttct tcctaacgaa acatcattac caatgataac aagtagacgc ccattaagta
      301 tttcatactc gcatccattc tatggtccgc tcaacaaacg aatggcataa gattccaata
      361 agtcttgtga taagaattta tcattatttt ccatgaatac cacccgctaa ttccataagc
      421 tctagtttag agtagtgtaa caagacggaa atttcatgac caaattatga tgagacagaa
      481 taaaaaaatt ataattttga ggaccatttt aaatgcctct atgcataact ttatttacaa
      541 tttaaggtat aaaccctatt ccctcaacgt taagcaaata taacaattac tcttatttta
      601 aattttacac ataatattta ccctaaatga ttttagagta caggttggca tatgcaagtc
      661 ttttccagtg atgtctactt cactgtaggc actaatgcat tattagcatc ccaaaaggaa
      721 tattacagcg atttagttgc gttggttgat cttggacatt cgttcgttgt tattgatgag
      781 catcaacatc gaaatctcaa accaaataca gaacctgtaa acatcctttt gagtaacaat
      841 ttcatctgga tcaataagaa tattacatta tctgatttaa cgcatttcct ggtttcaaat
      901 cttcatacac aaaatgtgta ttccacacaa gaaccactta cccatgatga gatagatatc
      961 ctgcggttat gtgtttcata ttcattaaag caaattgcca ttattaaagg aattgattat
     1021 aaaacagtat catatcacaa aataagagcc ctcaatcaac ttaacataaa aggtactgtt
     1081 gagttgttta tagcactctg tgaatgggat aaacattatt ttaaactcca atcttgcgta
     1141 agagaaagtt gatatgtcat tagtaatgat ataatagtaa aatcataata accatcgcgc
     1201 tgatatcgtt gctctctgca tgcaccagag aaaattcatt aaaggagaat caaaataaaa
     1261 gaagtggcga atcccaagcg atagtagatg caagacagtt ctttagcgaa cacccggaat
     1321 atatatcatc agcagaactg gaggaaaagc tatatcgcga atttgcacag gttactacag
     1381 ttccagctta taaagagatg agtatgtatc aattgcttgt catttcgcaa gatcgtttaa
     1441 ctcattaaat aaaaatctac acgttttttg cgtgtagatt cttataaatt tttgactacg
     1501 atacatgttt aattttttaa attat
//
LOCUS       DAZFDC010000334         1468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFDC010000334 DAZFDC010000000
VERSION     DAZFDC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.337"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..197
                     /locus_tag="ACHJ48_005080"
     CDS             <1..197
                     /locus_tag="ACHJ48_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4710404.1"
                     /translation="ETNSYGWFPGTDGALITGLTFLDPKDATQVQGMFQHLQVRFGDG
                     PWQDVKGLDEVGSDTGRTGE"
     gene            199..468
                     /locus_tag="ACHJ48_005081"
     CDS             199..468
                     /locus_tag="ACHJ48_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4710405.1"
                     /translation="MNILRKLMQSLCGCGKHDDCENGRSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            470..577
                     /locus_tag="ACHJ48_005082"
                     /pseudo
     CDS             470..577
                     /locus_tag="ACHJ48_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..>1468
                     /gene="nleB2"
                     /locus_tag="ACHJ48_005083"
     CDS             645..>1468
                     /gene="nleB2"
                     /locus_tag="ACHJ48_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO4710406.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELR
                     EISKAQDSLISMYAKKRNHAWFDFFRNLALLKAGEIFRCTYNTKNHGISFGEGCIYLD
                     MDMILTGKLGTIYAPDGISMHVDRRNDSVNIENSAIIVNRSNHPALLEGLSFMHSKV"
BASE COUNT          466 a          225 c          369 g          408 t
ORIGIN      
        1 ttgagacaaa cagctatggc tggttcccgg gtacagatgg tgcgctcatc accggactga
       61 cctttcttga ccccaaagat gccacacagg ttcaggggat gtttcagcat ttgcaggtca
      121 gatttggtga cgggccatgg caggatgtta agggactgga tgaagtgggc agtgatacag
      181 gcagaactgg agaatgacat gaatattttg agaaagctta tgcagagtct gtgcggttgc
      241 ggaaagcatg atgactgtga aaacggacgg tcacttacag cacaattgcg actgggaccg
      301 gcagacattc tggagtcaga tgagaatggc attattccgg agcaggacag ggtaatcaca
      361 caggtggtga tactggatgc agataaaaag cagatacagt gtgtggtaag accgctgcaa
      421 atcctgcgtg ctgacgggac gtgggaaaat attggcggga tgaagtaacc cgacagcttc
      481 acaaaaccgg agtccggctc cggtttttgt tgtcatgtcc ggtggatgtt tgttaggaaa
      541 gcaaagatgg caaaactgct ggaggttttg tggttgagta tgccaatata attaatagat
      601 taaagagtta gttgtgaaga aaatatggat aaacaggacg acgaatgctt tcaccgataa
      661 ggacaacttt ccataactca gtaaatatag tgcagagttc accctgtcaa acggtttctt
      721 ttgcaggaaa ggaatatgag ttaaaggtca ttgatgaaaa aacgcctatt ctttttcagt
      781 ggtttgaacc taatcctgaa cgatataaga aagatgaggt tccaatagtt aatactaagc
      841 agcatcccta tttagataat gtcacaaatg cggcaaggat agagagtgat cgtatgatag
      901 gtatttttgt tgatggcgat ttttcagtca accaaaagac tgctttttca aaattggaac
      961 gagattttga aaatgtaatg ataatctatc gggaagatgt tgacttcagt atgtatgaca
     1021 gaaaactatc agatatttat catgatatta tatgtgaaca aaggttacga actgaagaca
     1081 aaagagatga atacttgttg aatctgttag agaaagagct gagggaaatt tcaaaggcgc
     1141 aggattcttt gatttctatg tatgcaaaga aaagaaatca tgcatggttt gatttcttca
     1201 gaaatttagc cttattaaaa gcaggagaga tattcaggtg cacatataat acaaagaatc
     1261 acggtatttc attcggggag gggtgtatct atcttgatat ggatatgata cttacaggta
     1321 agcttggtac aatatatgct cctgatggaa tttcaatgca tgtggatcgt cgtaatgata
     1381 gtgtaaatat tgaaaatagt gcaataattg ttaaccgtag taatcatcct gctctacttg
     1441 agggactttc ttttatgcat agtaaagt
//
LOCUS       DAZFDC010000335         1462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFDC010000335 DAZFDC010000000
VERSION     DAZFDC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.338"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..555)
                     /gene="escD"
                     /locus_tag="ACHJ48_005084"
     CDS             complement(<1..555)
                     /gene="escD"
                     /locus_tag="ACHJ48_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO4710407.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTLGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADKPLPVGKVIDIAGCCFIIGDIDHSLP
                     LSDVPERFSAKSRRKKRLILASVIGATFALSGAIGSYVLLSPKAEPPTFTRADVYQQL
                     KENKLHAITLVWHGKNIALYGRCES"
     gene            698..>1462
                     /gene="sepL"
                     /locus_tag="ACHJ48_005085"
     CDS             698..>1462
                     /gene="sepL"
                     /locus_tag="ACHJ48_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFO4710408.1"
                     /translation="MANGIEFNQNPASVFNSNSLDFELESQQLTQKNSSNISSPLINL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGSTIEKLLNDMQELLTLTDSDKIKE
                     LSLKNSGLLEQHDPTLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYARLLLDLLGE
                     DDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEIKDLPEREKH
                     LKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIF"
BASE COUNT          462 a          255 c          310 g          435 t
ORIGIN      
        1 tgattcgcaa cgcccataaa gcgcgatatt tttcccatgc cataccagag tgattgcatg
       61 aagtttgttt tcttttagct gctgatatac atcagctctg gtaaacgttg ggggttcagc
      121 ttttggggaa agcaaaacgt aactgccgat ggcaccagac aacgcaaaag tagcaccaat
      181 cacgctcgcc aggataaggc gttttttgcg tcgacttttg gcagagaacc tttctggcac
      241 atcagacaat ggcaaggaat ggtcaatatc accaatgatg aagcaacatc ccgctatatc
      301 aataaccttt ccgacaggta atggtttatc tgcttcatag ggcgtgggtt gcccatcaat
      361 ccaaaattca accggagata aaaggaaaac accttcctca cgtatatcaa gtaaaaactg
      421 attgaggcct tgttcaaggg gaaagtaaac aatatcgtta tcttccgtac ctagcgttag
      481 gtttcccatc ggaagttgta attcccgatt acgcatcgca ccattgagaa gttttatttt
      541 atatgaggat aacattttta aattttattc atcctggtgg ttgaacaatg agaaaaaata
      601 tggtgaactt acatcgtcta agaatagagt agaaaggaag ctgttgtaca gtactattta
      661 atggaatatt cataattaat gattacgtga gtttccaatg gctaatggta ttgaatttaa
      721 tcaaaacccc gcatctgttt ttaattctaa ttcattagat tttgaattag aatctcagca
      781 attaacgcaa aaaaattctt ctaatatttc ttcgccatta ataaatttgc aaaatgaatt
      841 agcaatgatt actagttcat cgctttctga aacgattgaa gggctaagcc tgggatatcg
      901 caaaggtagc gcaaggaaag aagaggaagg ctcgacaatc gagaagctgc tcaatgatat
      961 gcaagagctg cttactctta ccgatagtga taaaataaaa gaactatcat taaaaaacag
     1021 cgggctgctg gaacaacacg atcctacttt ggcgatgttt ggcaacatgc caaaggggga
     1081 aattgttgcg ctaatatctt ctttattgca atctaagttt gttaagattg aattaaaaaa
     1141 gaaatatgcc aggttattat tagatttatt aggcgaagat gattgggagc tggctctgct
     1201 ttcctggtta ggggtgggtg agttaaatca ggaaggtatc cagaagatca agaagcttta
     1261 tgaaaaggct aaggatgagg attctgaaaa tggcgcctct ttacttgact ggtttatgga
     1321 gattaaggat cttcccgagc gtgagaagca cttaaaagtc attattaggg cgctgtcgtt
     1381 cgatctctct tatatgtctt cttttgaaga caaagtaaag acatcttcaa ttattagtga
     1441 tttatgcagg gtaatcattt tt
//
LOCUS       DAZFDC010000336         1427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFDC010000336 DAZFDC010000000
VERSION     DAZFDC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.339"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..1318
                     /locus_tag="ACHJ48_005086"
                     /pseudo
     CDS             90..1318
                     /locus_tag="ACHJ48_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            1329..1427
                     /locus_tag="ACHJ48_005087"
                     /pseudo
     CDS             1329..1427
                     /locus_tag="ACHJ48_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          387 a          328 c          405 g          307 t
ORIGIN      
        1 gactggcccc ctgaatctcc agacaaccag tatcacttaa ataagtgata gtcttaatac
       61 tagtttttag actagtcatt ggagaacaga tgattgatgt cttagggccg gagaaacgca
      121 gacggcgtac cacacaggaa aagatcgcaa ttgttcagca gagctttgaa ccggggatga
      181 cggtctccct cgttgcccgg caacatggtg tagcagccag ccagttattt ctctggcgta
      241 agcaatacta ggaaggaagt cttactgctg tcgccgccgg agaacaggtt gttcctgcct
      301 ctgaacttgc tgccgccatg aagcagatta aagaactcca gcgcctgctc ggcaagaaaa
      361 cgatggaaaa tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa aagtggatag
      421 cgcacgcgcc cttattgccc ggggatgggg agtaagctta gtcagccgtt gtctccgggt
      481 gtcgcgtgcg cagttgtacg tcattctcag acgaaccgat gactggatgg atggccgccg
      541 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
      601 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      661 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      721 tgagcgaaaa cctactgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      781 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      841 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      901 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      961 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
     1021 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
     1081 ggtgcggagt ccggagagta acggaatagc agagaacttc gtgaaaacga taaagcgtga
     1141 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
     1201 gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct cgccacggga
     1261 atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg aaatataggg
     1321 gcaaatccag atggatttat cgtaataaag acaacagcca aataatgaaa aacataaaat
     1381 tttatctgca tggcaaagag ataccagcag aaagaatatt agataca
//
LOCUS       DAZFDC010000337         1421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFDC010000337 DAZFDC010000000
VERSION     DAZFDC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.340"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            128..232
                     /locus_tag="ACHJ48_005088"
                     /pseudo
     CDS             128..232
                     /locus_tag="ACHJ48_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(352..1371)
                     /gene="nleC"
                     /locus_tag="ACHJ48_005089"
     CDS             complement(352..1371)
                     /gene="nleC"
                     /locus_tag="ACHJ48_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO4710409.1"
                     /translation="MICRVLDINMKIPSLQSNFNFSAPAGYSAPIAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEKVHIGCIKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEA
                     GENDNEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVD
                     KHNQQ"
BASE COUNT          389 a          315 c          283 g          434 t
ORIGIN      
        1 tctttctctc acgcaaaatt cacaaaatat tgttgctgca atggacctgt acaatagtga
       61 atcacggtac ttttctgtgc acaacgtact gcagtttctt tacgatttat agctgtactg
      121 ttgaattatg tacaatttga atccatgcat gacgtgtgat gcctattgtg catttttccg
      181 agcctctttt tactgggccg aagccgatga tgctgatagt ctcgttccgg ccatgttcac
      241 ttactacagt aaaattaggg gaattgcctt tacatacaat gttgcacgat tacccatcat
      301 caactccgtt aaaatgtatg aatagtaacc ttatgtcact gcaaagacga atcattgctg
      361 attgtgtttg tccacatccc caaatgtaaa aatataaggg gcgccatgat acagttggat
      421 ctgatttgca tcctgtaaat tatcgttgat agccaatcca ggaaaatcat gttgctggat
      481 aaatccgtat cctatgttag acacagcgga atactctgtg aaatcaggac tcgcctcata
      541 tcggtcactg atcgtaccca gcctttcgaa gaaagccctc tcattctctt catgcctcat
      601 ggcagcctgt cgaagggcat tcaggttacg tagcctaaga tgagcttcac gttctggctc
      661 tgcatatcct ttgaagtcgg gaacactcca tcccagttct tgagcgacac gacgtgcgag
      721 aatctcggtg ggtcctaact ctatattact atctccagat ggatcgctag atccagtaac
      781 atgatgaata atctcgtgaa tcagtccttc ctgccacgat ggcatctcat aagagtcagt
      841 atctggcgca acactaaaac tgacataagg ctcttcgtta tcgttttctc ctgcttcgca
      901 aatggggaaa attggctctt gcccgacatc atattcatat aattcgttac aggttagtga
      961 ttgaatatca tcaattttga cagaagattc ttcgttaagc tcgtattcgt ttctgtattt
     1021 aatgcaacca atgtgtacct tctcattatg aatgccatag cttacggcat cacgaaatgt
     1081 ctgtgatcgg ctcaaagcat caagtacagt gttgccgatc atatcgactg tatgctgatc
     1141 gataagcctg ctacggctgt catggacggc gcgtattaca ctttcgtata cgttgcttaa
     1201 atctgctgcg gaaagtggta ttcgcttacc tatatccaaa acgtaatccg cataggcatt
     1261 ttcagcacga ttaggagcaa tgggagcaga gtatcctgcc ggggcggaaa agttgaagtt
     1321 ggactgtaat gagggaattt tcatgtttat atctaatacc ctgcaaatca tttattacaa
     1381 caaaataaac tatatcaaat tcatttacca tgaactgcat g
//
LOCUS       DAZFDC010000338         1419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFDC010000338 DAZFDC010000000
VERSION     DAZFDC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.341"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..379
                     /locus_tag="ACHJ48_005090"
     CDS             <1..379
                     /locus_tag="ACHJ48_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO4710410.1"
                     /translation="RGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIKVP
                     LTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRS
                     NNCYGQVSRRDQESALACWGIDR"
     gene            539..676
                     /locus_tag="ACHJ48_005091"
     CDS             539..676
                     /locus_tag="ACHJ48_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710411.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKYILIWLVFVIMASGYFIGQIRW
                     W"
     gene            678..1172
                     /locus_tag="ACHJ48_005092"
     CDS             678..1172
                     /locus_tag="ACHJ48_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO4710412.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQVEGAQDYIRTQCTKQAFYY
                     PEDV"
     gene            1169..1393
                     /locus_tag="ACHJ48_005093"
     CDS             1169..1393
                     /locus_tag="ACHJ48_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710413.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
BASE COUNT          377 a          307 c          398 g          337 t
ORIGIN      
        1 ccgtggagcc acccgggtgg atggtaagcc tgttattcct ggcatgaagc tgtcgaaggg
       61 gaaatgcgac caggttaacg ccattgagcg tgataaggcg ctggcatggg tggagaaaaa
      121 catcaaagtg ccactgactg aaccccagaa agcgggtatt gcgtcattct gtccttacaa
      181 cattggccca ggtaagtgtt tcccgtcgac gttttataaa cgaattaatg caggagatcg
      241 aaaaggtgcc tgcgaagcga ttcgctggtg gattaaggac ggtggcagag actgccgtat
      301 tcgctcaaat aactgttatg gtcaggtctc acggcgtgac caggagagcg cgctggcgtg
      361 ctggggaatt gacagataag cagaatattt tgctgaaaaa tgcggtttgc tcacacggac
      421 ggataacaca aaatcctgcg aactgacaaa aactaagtga ataaaagtaa aaaccccatt
      481 tgttggctgc aagcggggtt ttgtgtttcc tgactccgga aaagtcaaag gagaaagtgt
      541 gtttgatttt agcaaactga ttcgggagat tcgagtgatg gctgaaaaat tatcaacctg
      601 gaagtacatc cttatctggc tggtgtttgt gattatggcc tccggttatt tcatcggtca
      661 gatacgctgg tggtgaaatg aaccgcgtac tgtgcgtggt catcattgcc ctgctggtgg
      721 cctgtggtgc gcttagtctg gggctgaatc attaccgcga taacgccatc acctacaaag
      781 cgcagcgcga taaaaaagcc agagagctgg agctggcaaa cgcaactatt actgatatgc
      841 aggtgcgcca gcgcgatgtt gctgcgctcg atgcaaaata ctcgagggaa ttagccgatg
      901 cgagagctga aaatgaaact ctgcgcgctg atgttgccgc tggtcgtaag cgcctgcgga
      961 tcaacgccac ctgccccggt accgtgcgtg aagccaccgg cacctccggc gtggataatg
     1021 caaccggccc ccgactggca gacaccgctg aacgggatta tttcatcctc agagaacggt
     1081 tgatgacaat gcagaagcag gtggaagggg cacaggacta tatccgcact cagtgtacta
     1141 agcaggcttt ttattatccg gaggatgtat gaagaaatta caggtaacgg taaaaccttt
     1201 tcagggaaca attccgttcc gtgttttgca acatggccgt gttctgcttg aagaggtgtt
     1261 cagaggtaaa tgcactgaat gttattcacg aacatatgaa gtgaatgcca cgcatgaaga
     1321 attcaccgtt gagtgtgtga tgaatactga taaatgccga atggtatccg ctgaattaca
     1381 gccagtgtgt tgagcgacct tattatccat gcgcggtat
//
LOCUS       DAZFDC010000339         1414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFDC010000339 DAZFDC010000000
VERSION     DAZFDC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.342"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1414)
                     /locus_tag="ACHJ48_005094"
     CDS             complement(<1..>1414)
                     /locus_tag="ACHJ48_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4710414.1"
                     /translation="ARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPKVV
                     KGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPA
                     SVSFRIAAPAAPSQIELTPGYFQITAVPRLAVYDPTVQFEFWFSETRITDIRQVETTA
                     RYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGE
                     IGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKV
                     QVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADRIAMINPAN
                     GNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTSDGKLTAKNADISGSVN
                     ANSGTLNNVTINENCRVLGKQSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVY
                     DDQPFDRQIVIPAVAFSGAKH"
BASE COUNT          290 a          425 c          355 g          344 t
ORIGIN      
        1 catgtttagc gccgctgaat gccaccgccg gaataacaat ctgccggtca aacggctgat
       61 cgtcataaac cctgacggta atggtccctg atggccaccg ttccggtgca cgggagtccc
      121 gggggaaagc tttgcccact gttttaacga gatcgccttc aatctggttc gcggactgtt
      181 ttcccagaac ccgacagttc tcgttaatcg tgacgttgtt gagcgtcccg gagttcgcat
      241 tcacactgcc actgatatcc gcatttttag cggtcagctt tccgtctgat gtcagggaaa
      301 atgccggagg attgccgcca ctggtaatgg tgggagccgt cagatatttc aggaacactt
      361 cattcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg ccattcgcag
      421 gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatg ccgtcagagg
      481 tgttctcaat accggcaccg atacccgcaa tataaaggcg tccgtcctgc atctgctgca
      541 gcttcactgc ccacatgctg ttcaggttat tatttgtatc aacctgaacc ttctgtatct
      601 gctggatcgc tgcactctgg tcttccagtt tcttattgac ggtctgcgtg atttcattac
      661 tgacatccgt gatggacgtt ctgatttccg ccaggtcagg cgcaagctga ccgttatcaa
      721 tctgagtcca caactcctga gccagatggg ttttccctat ctctcctttg aaaaaatcca
      781 gatagccgga tgcatcatca ctcggctggc caacagcctc cacaaatgcc gatttgccaa
      841 cggtgttcac actgcggata taaaagtaat aatcatggcc cggtttgata ttgatactgg
      901 cggctatcca gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc tgcctgatat
      961 cggtaatccg cgtttccgaa aaccagaact caaactgtac cgtcgggtca tacaccgcaa
     1021 gacgcgggac cgctgttatc tgaaaatagc ccggtgtcag ctcaatctgt gacggcgctg
     1081 ccggtgcggc aatccggaac gataccgacg ccggatcgcc ctgctgcccc cacgcattta
     1141 ccgcccggac tgtcagcctg tagttccccg gcgccagttg cgtgaagcgg tatgtggttt
     1201 ccgtcgtccg ggccgtgctg accagccgct cactgccgtc atccgcggcc acggtcaggc
     1261 gaagcataaa gctcacgccc ttcaccacct tcggcgtgtc ccatttcgcc tgcgccagat
     1321 actgaccgtc agctgcgctc acctccaccg tcaggtgctg cactgccgga gggatgacgc
     1381 tgttcagggt gcctgactgc ggctcaaagc gggc
//
LOCUS       DAZFDC010000340         1403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFDC010000340 DAZFDC010000000
VERSION     DAZFDC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.343"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            259..>1403
                     /locus_tag="ACHJ48_005095"
     CDS             259..>1403
                     /locus_tag="ACHJ48_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO4710415.1"
                     /translation="MVDNVTVSRVCIQSPSFVPDLDGEKNKSQLFVDDIVAYLKSPSV
                     YSLEKEGPLNHFVNHCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPVLIKFLDDIFTCLHTYPDDESFTKEQIKANSKYDIVDYNCLLHFTGKPKSLIEC
                     RHFALQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFNNLQKNPPPGTCVVA
                     SDKFGEALSVFFHRMEKEKLTHMTAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLQSFINIQQAKQKWVITDICSECVGITPYLPREQAHLLSG
                     IENELQPPLSPPALFLLMRMGIYKNIVLFFDKLKNSQEMTASKALDIL"
BASE COUNT          470 a          238 c          255 g          440 t
ORIGIN      
        1 aaaatacgtg aatggataat ttttttaatt tttataatta ttgttgaaga tgatgatatt
       61 gattataggg ataccattac aaggagctgt tcccctcgtt gacgtcgcgg tttacccacc
      121 tcattaccag gttgatgacc tgatggggaa atgacgtaaa aagacaaata actctataaa
      181 tgagcgaaat ttgatagttg atttttaaat agtaacctta ttattactac aactttcata
      241 tggatgaggg gaaagaaaat ggttgataac gtaactgtta gcagagtatg tatacaatct
      301 ccttctttcg tgcctgattt ggatggagaa aaaaataaat ctcaattatt cgttgacgat
      361 atagttgcgt atcttaaaag tccttcagtt tattcacttg aaaaagaggg gccgttaaat
      421 cattttgtta atcattgttc tgaagttgag ttaggtttct atagcgatgg tgcatattca
      481 attcttgtct ccagatctaa gcaacaacct gaaggtatga ttttaaccgt aagcgatgca
      541 gacgcaatca atatagtaca tatttccgta tctccagtgc ttataaaatt cctggatgat
      601 atttttactt gccttcatac gtaccctgat gatgagagtt ttacaaaaga gcagataaaa
      661 gctaatagca aatatgatat tgtagattat aattgcctgt tgcattttac tggaaaacca
      721 aaaagtttaa tagaatgtag acattttgct ctgcaatact gtatagattc aatgaatgag
      781 catacaggga aagttccatt aaaggcttac tattcatctc cggaagatat acaaaaacat
      841 attcctttcg agcttgagca gcaatttaac aatctacaaa aaaatccacc acccggtaca
      901 tgcgtcgttg ccagtgataa gtttggggag gcattatctg tcttttttca cagaatggaa
      961 aaagagaagt taacgcatat gacggcaatc gttcaatctc aaacacacgc tatggccgtc
     1021 cgcttgagga tcaaaaaaac gcctgctggt gaaacagaat atgttgtatc cttttatgat
     1081 cctaatgcaa ccaatactgc agtacgctat aaagcaaaca actgtgattc ttttgggtca
     1141 ttgcaatcgt ttataaatat tcaacaggca aaacaaaaat gggtaataac agatatttgc
     1201 tccgagtgtg taggaataac cccttatctc cctcgggaac aagcccattt attaagtggt
     1261 attgaaaatg agttgcaacc tccattatca ccaccagcat tatttctatt aatgagaatg
     1321 gggatatata aaaacattgt tctttttttc gataaattaa aaaactctca agaaatgaca
     1381 gcatcaaagg ctcttgatat tct
//
LOCUS       DAZFDC010000341         1399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFDC010000341 DAZFDC010000000
VERSION     DAZFDC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.344"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..654)
                     /locus_tag="ACHJ48_005096"
     CDS             complement(<1..654)
                     /locus_tag="ACHJ48_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO4710416.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLRKQNTDCHDESRRVTHMSRQITTDTDTDKE
                     LNPTHNARMRESATTGELNDASLQTAEPEYLDGLSEPIGKFPMTGVWQPSPDFRQRAA
                     "
     gene            complement(723..1148)
                     /locus_tag="ACHJ48_005097"
     CDS             complement(723..1148)
                     /locus_tag="ACHJ48_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO4710417.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAAAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(1145..1372)
                     /locus_tag="ACHJ48_005098"
     CDS             complement(1145..1372)
                     /locus_tag="ACHJ48_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO4710418.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTQGEGKGDS"
BASE COUNT          310 a          404 c          310 g          375 t
ORIGIN      
        1 tgctgcccgc tgtcgaaaat ccggcgacgg ttgccagact ccagtcatcg gaaatttccc
       61 gatcggttcg ctcaggccgt ccaggtattc aggttcggct gtctgcaacg acgcgtcatt
      121 caactcaccg gtcgtagcac tctcgcgcat gcgcgcgtta tgtgtggggt ttaattcttt
      181 atctgtatct gtatctgtcg tgatttgtcg tgacatatgc gtgacgcgtc gtgactcatc
      241 gtgacaatca gtgttctgct tccgcagtct ttcccgctcc cgctgcgctc tcttgcgctc
      301 tgccggggat tttgcggttt gcgaaacatt accgttatcc tccttcatca cctggcgttt
      361 ttcccatccg gaaataagat caccatccag aactcgcccc tgcattgcat gcaaaattga
      421 atcaatcacg tcttccgtca catcaagcgc acttgctaaa tcttccgtcg tgacatcaat
      481 gtgaccacgt agtgacacgc cgtgacatgt cgtgacattt cgtgacgcac tcaccagaag
      541 gtggatatac accgccatca ctgttgcgat tggctgtcct gagaccctgg caatggttcg
      601 ccatttggga tcatttggca tgtcatgcca caatctgagc caggcattag ccatactcac
      661 ctcatctgat accgaacttt accctcgaac atccggaaga aatccggcat gaatattgtt
      721 ggtcaatgca cgacaacagc attaccaggc tgaccaccac tgttagtcag ggtgccccag
      781 gcgatcgccg ctgcgacaaa atcatccaca tctttcacca gccgatccct ccgttcgacg
      841 atctcacggt aatattcaga gctgtgactg cgcatacggg ccaccagcag aggcggcatt
      901 gccttttcga tcgccggtaa cagagcctga attttttcaa cagcatcagg ggtgtcttta
      961 tccagccaac ggaaaatttt ctgggtatta cgagccaggg cttccggatg gctgtcgtcg
     1021 tacagttccg ggaacgtcat ccccagctcg aaataagtcc gggctatttc agctgcagga
     1081 actttctcac cgtccggata ggcccaggca ttgatcgcca tgcggatgtg ctcatgtttg
     1141 attttcatga atcccccttt ccttcgccct gagtggtatc cttctttttg taaagttctg
     1201 ggttcaaaga taatttcccc ttggagtatg cagcagcttc cgcagccctc cccttcggaa
     1261 ctatttcacc aggacgctta cgccacatgt aaatagcttc gcgggttatc ccataaaaat
     1321 cggcaaccct ctgaacagaa ccaaaaaact ggacaagttc atcaactcgc attttacctc
     1381 ccaaatctaa gtattttta
//
LOCUS       DAZFDC010000342         1385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFDC010000342 DAZFDC010000000
VERSION     DAZFDC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.345"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..218
                     /locus_tag="ACHJ48_005099"
     CDS             <1..218
                     /locus_tag="ACHJ48_005099"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4710419.1"
                     /translation="LAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEIN
                     IVRAQPVSGGRLGVKIYPIMLEGQVKK"
     gene            215..1036
                     /locus_tag="ACHJ48_005100"
     CDS             215..1036
                     /locus_tag="ACHJ48_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO4710420.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDFRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1274..1339
                     /locus_tag="ACHJ48_005101"
                     /pseudo
     CDS             1274..1339
                     /locus_tag="ACHJ48_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          372 a          273 c          399 g          341 t
ORIGIN      
        1 ggctggcgat aaagattatt gccgagccac cggataagcg ccgccgtgac ctggacaata
       61 ttctgaaagc gccgctggat gcgctgacgc atgcggggtt gctaatggac gatgagcagt
      121 ttgatgaaat caatatcgtt cgtgctcagc cagtatctgg tggacgtctg ggggtgaaga
      181 tttaccccat aatgcttgaa gggcaggtca aaaaatgaaa ctggaagatt taccgaaata
      241 ctactcccca aaatcccccg gcctgactga tgcatcggcc tcaacgtcga aagatacgct
      301 gagtatcact gatgtgatgg ccgcgcaggg catgacacag aattgggctg agatggggtt
      361 ttctgcgttc cttgggaaaa tgggcattag tatgaatgac agagagcggg caacagaatt
      421 gctgacagaa tatgcactca gtcggtgtga tcgcgtggcg gcgttaagaa aactcccggc
      481 agaaataaaa ccggcagtga tgcgtattat ggcttcgtat gcgtttgaag attatgcccg
      541 tagcgcggcg agcaaaaaac agtgcccctg ttgtcacgga aaaaaattta ttgaaagcga
      601 ggtttttaca aacaagatcc agtatccgga tggtaagccg ccagtgtggg caaagtgcac
      661 aaaaggcgtg tatccgtctt actgggagga atggaaaaaa gtcagggagg tggtaaaagt
      721 tgcctgtccg gagtgtggag ggaaggggga ggtttccacc gcctgtaaag attttcgtgg
      781 gcgcggtgtt gccattcatc gtgaagagtc ggtaaaacgt ggtatgcctg ttatcagaga
      841 ctgccagcgt tgtggtggtc gtggctatga aagattacct tcaacggagg catttaatgc
      901 catatgtaat gtaaccgatg ccatatctct tgatacatgg aaaaaaacag ttaaacgttt
      961 ttacgatacg ctggtggtgc agtttgatat tgaagaagca tgggcagaac aacaactgaa
     1021 aaaggtgacc agatagcttt gttgattttt cccgaatctg tggtaaattt gccctaacga
     1081 tgggcgtttt atgcctgacg ttagaagatt ttttacaccc gtcgccaggc gggttttttt
     1141 atgactgaaa tcacgccagt acagtaaacg cgctggtggt tgtgaatacc ggtctttctg
     1201 cttgctggct ttttcgacaa gagttattgg tgtgtcacgt taaccggaaa aaggaaagtt
     1261 tgagaaacgc gatctggcac aggcggtgat taacgccgca tacctggtgg cctgtgcaga
     1321 tggtgaatgt gaggcttcct agaaagcgaa gatcgaacag gtactgcgta atcagccagc
     1381 gctgt
//
LOCUS       DAZFDC010000343         1372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFDC010000343 DAZFDC010000000
VERSION     DAZFDC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.346"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            76..>1372
                     /gene="nleA"
                     /locus_tag="ACHJ48_005102"
     CDS             76..>1372
                     /gene="nleA"
                     /locus_tag="ACHJ48_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO4710421.1"
                     /translation="MNIQPTIQSGITSQNNQHHQTEQIPSTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNV
                     TQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASTGTSATTSTVTTEPKDPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDI"
BASE COUNT          442 a          282 c          286 g          362 t
ORIGIN      
        1 catcggatat gtgacactaa tagtatcaat ggattgatat tattaatgga tataaacatg
       61 taataaggat ttattatgaa cattcaaccg accatacaat ctggaatcac ctcacaaaac
      121 aatcaacatc atcaaacaga acaaataccc tctacacaaa taccgcaatc cgaattacct
      181 ctaggatgcc aagctggatt tgttgttaat attccagatg atatacagca acatgcaccg
      241 gaatgcggtg aaacaacagc tctactgagc ttgataaaag ataaaggtct gctctcaggg
      301 ctagacgaat atatagctcc tcaccttgaa gaaggatcca taggaaaaaa aacattggat
      361 atgtttggtt tattcaatgt tacccaaatg gcattagaga tacctagttc cgtttcaggc
      421 atctctggta aatatggtgt ccagctaaac attgtaaaac cagatattca tcctacatca
      481 ggtaattatt ttttacagat attccctctg catgatgaaa taggttttaa ttttaaagac
      541 cttcctggcc cgttaaaaaa tgcattaagc aacagtaata tatcaaccac tgcagtgtcg
      601 actattgcat cgactggaac atcagccact acttcgacgg taaccaccga gccaaaagac
      661 ccaataccat ggtttggatt aacagctcaa gtggttcgta atcatggtgt agaacttcct
      721 atagtcaaaa ctgaaaatgg atggaagctt gttggagaaa caccacttac tcctgatggg
      781 ccgaaagcaa attacacgga ggagtgggtt atcagaccgg gagaagcaga ttttaaatat
      841 ggtgcatctc cattacaggc aactctaggg ctggagtttg gcgcacattt caagtgggat
      901 ttagataacc ctaatactaa atatgccgtt cttaccaatg ctgccgcaaa tgcgcttggt
      961 gctttagggg gatttgcagt atccagattt gctagtacag atccaatgtt aagtcctcat
     1021 atcggtgcaa tggttgggca agcagcaggg catgccatac agtataatac ccctggatta
     1081 aagccagaca ctattttatg gtgggctggt gcgacactgg gggctgccga tttaaacaag
     1141 gccgagtttg aagtagctag attcactgac tatcctcgta tatggtggca cgcaagagaa
     1201 ggagctattt tccccaataa agcagatatt gaacatgcca caggtgctga tatacgcgca
     1261 atggaagaag gtatccctgt tggacagcgg catccaaatc cagaggatgt ggtaatcgat
     1321 atcgaaagca atggcttacc acatcataat ccatcaaatc atgttgatat ct
//
LOCUS       DAZFDC010000344         1353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFDC010000344 DAZFDC010000000
VERSION     DAZFDC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.347"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            280..587
                     /locus_tag="ACHJ48_005103"
                     /pseudo
     CDS             280..587
                     /locus_tag="ACHJ48_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(836..1291)
                     /gene="espF"
                     /locus_tag="ACHJ48_005104"
                     /pseudo
     CDS             complement(836..1291)
                     /gene="espF"
                     /locus_tag="ACHJ48_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
BASE COUNT          401 a          312 c          284 g          356 t
ORIGIN      
        1 aacaaaaccc cttttaatcc cttatatatc aggctttctt tgtgtacagg ggtcaaccga
       61 ctcaacctgt atcaagttgc ttttgaggat actttagggg gtattatttc ctcttgaaat
      121 attagcaccc cataatggga aaaaggtaat acataacaat ggtttttaac agtatctttg
      181 ttatacaagg gggcattgag gatatcagga aaaatctcaa aatcggtacg gacaggaaac
      241 tacgtgggca gcaaaaacgt atggtaacac cgggaaatgc aacgcccacc agtcgggcta
      301 atccaccact ccaatcgtgg ttcgcagtac tgcacacacg attaccgggc tatacaggag
      361 cggtttggtc tgaaaacatc catgccgcgt aaaggtaacc gttacgacaa cacctcaatg
      421 aaaaacttat agtaaacgct gaagaatgaa actatcgttt taataaccgg gatgaagcca
      481 tctaagtaat acggaaatac attgagattt tctacaacca tcagcgccgc cactcccgtc
      541 tggggaatat ctccctggta gccttcagga aaaatataac cagataactg cgtaaaaaaa
      601 gaacaaatgg tggtgtccgc tattgccagt atacctgagt ttactgcctt tcagattatc
      661 acaatcaata gcgaacagac ttttaagctc tgtgcgcttt taacaaaacg tccctttctc
      721 cactttcacc gtataataat acattattta ttacccctcc aataaaaacc gatattacta
      781 atcacattta ttaccacaca atttgatatc ggtttttatt agttggttac cctttcttcg
      841 attgctcata ggcagctaaa tgatctttta atgcctgtgc aaggggcggt agtccactag
      901 gccctgtcgg tggcggtggt gcctgacggg cgggcttaaa acctaaagcc tcaggacctt
      961 tcgatttttc ataggcagcc aagtgctctt ttaatgcctg tgcaatgggc ggtaaaggtc
     1021 gggatgcccc ggatgcctgt ccacttgtcg gtggcggcgg tgccggacga gagggagtaa
     1081 atgaagtcac ctggctgctc acattaaaaa tcgttctcgc attaacattc gacgagcctg
     1141 gagaaaaagg ggaatgaact ttcaccggag taagacgcac ggcctgaggg gctacagaaa
     1201 atccagttcc ccccgcagag ctcactcgac ttgcgatacc tacaagctgc cgccctagtg
     1261 tagaagcagc gttactaatt ccattaagca taatttatgc ctctttatat ctaagcttgg
     1321 ctaactctca tatatatcaa aacgaataat aaa
//
LOCUS       DAZFDC010000345         1339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFDC010000345 DAZFDC010000000
VERSION     DAZFDC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.348"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1222
                     /locus_tag="ACHJ48_005105"
     CDS             <1..1222
                     /locus_tag="ACHJ48_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4710422.1"
                     /translation="PLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEVTWYGW
                     DGDRLTTVQTDTTRIQTVYEPGSFTPLIRVETENGEREKAQRRSLAETLQQEGSENGH
                     GVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHF
                     YHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLY
                     YNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDD
                     SYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAA
                     ITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASV
                     MTLACRTWQECWVN"
     gene            1234..>1339
                     /locus_tag="ACHJ48_005106"
     CDS             1234..>1339
                     /locus_tag="ACHJ48_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFO4710423.1"
                     /translation="MKKYIFLVLFLLYGCSPVDIEPARQEVNIFRGLYQ"
BASE COUNT          341 a          274 c          417 g          307 t
ORIGIN      
        1 gccactggtc gagagccgct acctctacga cccgctggga cggcgaatgg caaaacgggt
       61 ctggcggcgg gagcgtgacc tgacggggtg gatgtcgctg tcgcgtaaac cggaggtgac
      121 gtggtatggc tgggacggag acaggctgac gacggtgcag actgacacca cacgtatcca
      181 gacggtatac gagccgggaa gcttcacgcc gctcatccgg gtcgagacag agaacggcga
      241 gcgggaaaaa gcgcagcggc gcagcctggc agagacgctc cagcaggaag ggagtgagaa
      301 cggccacggc gtggtgttcc cggctgaact ggtgcggctg ctggacaggc tggaggaaga
      361 aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg cttgcgcagt gcgggctgac
      421 ggtggagcaa ctggccagac aggtggagcc ggaatacaca ccggcgcgaa aagttcattt
      481 ttaccactgc gaccaccggg gcctgccgct ggcgctcatc agcgaagacg gcaatacggc
      541 gtggcgcggg gagtatgatg aatggggcaa ccagcttaat gaagagaacc cgtattacct
      601 gcaccagcca taccgtctgc cggggcagca gcatgatgag gaatcagggc tgtactataa
      661 ccggaaccgg tactatgacc cgctacaggg gaggtatatt acacaagacc ccattgggct
      721 ggcgggggga tggaatctgt ataattaccc actgaatccg ataataagga tggatccttt
      781 gggtttgtat aatttatatc aattattata tgatgtttgg catgatgatt catatggaac
      841 atcatcaatt gatattactg gcagtggaga tctaatatca ttaggtggtc atgcaggact
      901 tggcgttgcg tttgctaaaa agaaaggtga aatgttatct gatatttgta tttatgctac
      961 agcatgcgga catgcaggaa ttggtggtgg gataaatgcg gctatcacat attctgagac
     1021 caagtcttta cctacatcgg gagtcagcaa ttcagtaggt gtaacggttg gcggcggagt
     1081 tggggggcat tttgcgtata cttatgtagt ggatgttgat aatccagaat catcgacaga
     1141 atctgttggt atcggtgcag gtgttgacgc ttcagttatg actctggctt gtagaacgtg
     1201 gcaagaatgc tgggtcaatt aacaggagga aatatgaaaa aatatatatt tttagtttta
     1261 tttttattgt atggttgttc tccagtggac atagagccag ctagacaaga agttaatatc
     1321 tttcgtggtt tatatcaga
//
LOCUS       DAZFDC010000346         1332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFDC010000346 DAZFDC010000000
VERSION     DAZFDC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.349"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..162
                     /locus_tag="ACHJ48_005107"
     CDS             <1..162
                     /locus_tag="ACHJ48_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4710424.1"
                     /translation="NTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGG
                     KVVVIGRQA"
     gene            complement(353..880)
                     /gene="sodC"
                     /locus_tag="ACHJ48_005108"
     CDS             complement(353..880)
                     /gene="sodC"
                     /locus_tag="ACHJ48_005108"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO4710425.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            1014..>1332
                     /locus_tag="ACHJ48_005109"
     CDS             1014..>1332
                     /locus_tag="ACHJ48_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710426.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITS"
BASE COUNT          328 a          317 c          387 g          300 t
ORIGIN      
        1 aacacgtact tttcgtcgct ggacaacatg attgcccagg gtaacccgat gccggtgcct
       61 tacggggaaa tgctggttgg ctcccgccgt atatcccagg acatcagtac ccgtgatgaa
      121 ggcgggggcg gaaaggtcgt ggttatcggg cggcaggcat aaaagcgaaa aaatcccgca
      181 gtgctcacgg acaggaactg cgggagcgtt acgaagattg agtgtaagga attattctta
      241 tgtcacgaca aaaacattaa ctcagagagg gaggatgtgc cgttcttttc agggagaaag
      301 gatttatcgt cctgaggaat aaaggtaagg ggcccgcccc ttacctgact gattattgaa
      361 tgatgccgca ggccattctc gcaccaccac cgcccagggg ctccggatgg tcatgatggt
      421 tatcaccgcc agcatgaagc atgagagaac gccctttaat ctcttttaat gagttcagtc
      481 tcggggccag gaccgggtag ttcgcttttc cgtcatgcgt cacgaacagc gcagggaggt
      541 cgcccaggtg tccatccgga gaccaggggc caagatgttt gccggtgttt ttcgggtcaa
      601 agtgaccgcc agccgataat gctgcgaccg gttttccgtc tttcagtgcc ggggcgcaat
      661 ttcctttttc gtgcacatga aaaccatgaa tgccttcaga cagagagtga agggctggtg
      721 tgaacagcag accgtagggg gtctcctgaa tggttatttt tccaatgctg acttcttttc
      781 cgtcagcact gacaaggttc attgggactt cctgttctgc tgcgtatccg catgatgctg
      841 ctgtcagcat ggcaatggca gcaatgattt tacatttcat aaaaccctca ttaattccgt
      901 taacagactg agcttgctgg ttacagggta acaaacagcg ttctgatgat atcgcgcaat
      961 agctgtgcaa tatcctatca ctgcgattaa taataccaat tgagaggaac attatgggca
     1021 aaggtggcgg cagggcgcac acgcctcgag aggcgaagga taatctcaaa tccacgcaga
     1081 tgatgagtgt gattgatgcg attggtgagg gaccgataga aggcccggtg aaggggctgc
     1141 agagtatcct ggtgaacaaa accccgctga cggacacgga cggtaatcct gtgatacatg
     1201 gtgtgacagc ggtctggcgc gccggggagc aggagcagac accacctgaa ggctttgagt
     1261 cttccggagc tgaaaccgca ctgggcgtgg aagtgacgaa ggcaaagccg gtgacgcgca
     1321 ccattacatc cg
//
LOCUS       DAZFDC010000347         1322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFDC010000347 DAZFDC010000000
VERSION     DAZFDC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.350"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1322
                     /locus_tag="ACHJ48_005110"
     CDS             <1..>1322
                     /locus_tag="ACHJ48_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4710427.1"
                     /translation="TLNSVIPPAVQHLTVEVSAADSQYLAQAKWDTPRVVKGVRFSLR
                     LTSGSGQDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPAITTFRINAP
                     AKPATIELTPGYFQITAVPVLAVYDPTVQFEFWFSEKRITNTAQVEKSARYLGSGSQW
                     TVQGSRIKPGTDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHLAQG
                     LWELIDNSQLADEMAEMKTTITETRNEITQTVSKTLENQSATIQQIQRVQKDTNDDLA
                     ALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVA
                     QGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPDGRLTAKNADISGSVNANSGTLNNV
                     TINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTYASGTITVRISDDQ"
BASE COUNT          341 a          329 c          402 g          250 t
ORIGIN      
        1 accctgaaca gcgtcatccc tccggcagtg cagcacctga cggtggaggt gagtgcagct
       61 gacagccagt atctggcgca ggcgaaatgg gacacgccgc gggtggtgaa gggcgtgcgc
      121 ttcagtctgc gcctgaccag tggaagcggt caggacagcc gtctggtgac caccgccatc
      181 actgcggata cagagcatcg tttcagtggt ctgccgctcg gggaatacac cctgacagtc
      241 agggcaatta acagttatgg ccagcagggg gaaccggcca tcaccacctt ccggattaac
      301 gcgccagcaa aacccgccac cattgaactg acgccggggt attttcagat aacggcggta
      361 ccggtgctgg cggtgtatga cccgacggtg cagtttgagt tctggttttc ggaaaaacgc
      421 atcacgaaca cggcacaggt ggaaaaatct gcccgttatc tggggagcgg cagtcagtgg
      481 actgtccagg gaagccggat taagccgggg acggatttct ggttttacgt gcgcagcgtc
      541 aacctggtgg ggaaatctgc gtttgtggaa gtcagcgggc agcccagcaa tgatggtgaa
      601 gggtatctgg aatttttccg ggaaaaaata ggaaaactgc atctggctca ggggctatgg
      661 gagctgatag acaacagcca gcttgcggat gagatggcgg agatgaagac caccatcacg
      721 gaaacccgca atgaaatcac acagacggtc agtaaaacgc tggagaacca gagcgccact
      781 atacagcaga tacagcgcgt gcagaaggac acaaatgatg acctggctgc gctgtacatg
      841 ctgaaggttc aaaaaacgaa agacggcatt ccctatgtgg ccgggattgg tgcagggatt
      901 gaggatactg atggccagcc actgagcaac atactgctgc tggctgaccg tatcgcgatg
      961 ataaatccgg agagcggcaa cagcacgccg ttatttgtgg cgcaggggaa tcagctgttc
     1021 atgaacgacg tgttcctgaa gcgactgttt gcggtgagta tcacctcgtc cggcaatccc
     1081 ccgacgtttt ccctgacgcc ggacgggcga ctgacggcga aaaatgcgga tatcagtggc
     1141 agtgtgaatg cgaactcagg gacgctcaac aacgtcacga ttaatgagaa ctgtcagatt
     1201 aaggggaaac tgtcagccaa ccagattgaa ggcgatattg tcaaaacggt cagcaagtct
     1261 ttcccccgca cgagcactta tgccagtggc accatcacgg taagaatcag tgatgatcag
     1321 aa
//
LOCUS       DAZFDC010000348         1314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFDC010000348 DAZFDC010000000
VERSION     DAZFDC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.351"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..>1314
                     /locus_tag="ACHJ48_005111"
     CDS             54..>1314
                     /locus_tag="ACHJ48_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO4710428.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEILEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENTFKNIEMAIADIKREYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLACRTIQQNTPQKNIDFINLYNE
                     IRKKYNNNPSGQQEYLNKLRELYATYQK"
BASE COUNT          524 a          227 c          225 g          338 t
ORIGIN      
        1 aatattctct cacttattaa aacataaata attttttaag cggaaatgta attatgagac
       61 tgccagagaa agttcttttt cctcctgtca ctagtggcct gtcagggcag gaaaaacaaa
      121 aaaaaccgaa gagcattacc ggatttcagg aaaattatca acgcaatatc aggccaatca
      181 aaacagcatc agaagcccga ctacgcttct ttgataaaat ggtttcgaaa gaaaactctc
      241 tggaagatgt tgtttcttta ggtgaaatga ttcagaagga aatttatggg catgaacaaa
      301 gaacattttc accagttcat catacaggta actggaaatc atcattgtta cacaacgcgc
      361 tccttggtct ggcaaatgtt tataatggct tacgggaaac agaataccct aacactttca
      421 acagagatgg tataaaaagt actaactctt ttagagataa cttattgaca aaaacaagaa
      481 ctcccagaga taattttgag gaaggaataa aacatcctga acatgcaaca ataccatatg
      541 acaacgacaa tgaaagtaat aaattgctaa aagcaggaaa gatagctggt aacaataacg
      601 agctgttgat ggaaataaaa aaggaatccc aaagcgacca tcaaatcccc ctgtcagata
      661 agttcctgaa aaggaaaaaa cgatctcctg tagctgaaga taaagttcaa aactcgttaa
      721 caccagaaaa ttttgttcag aaaatttcac ttagtgatga gcttaaaaca aaatatgcaa
      781 atgaaattct agagataaaa agaataatgg gagaatacaa tcttttaccg gataaaaaca
      841 gtcgtaatgg tctaaaactt ctacagaagc aagctgattt actaaaaata atcatggagg
      901 atacctctgt tacagaaaat acctttaaaa acatagagat ggctatagca gatataaaaa
      961 gggagtacta ttcgcataca gttgatattg agaaaaatat tcatgccata tgggttgcgg
     1021 gttccccacc tgaaagcatc tcggactata ttaagacttt tctcaaaacc tacaaagagt
     1081 ttacttacta tctttgggtt gatgaaaaag catttggagc cgcgaaattt accagtgtct
     1141 taaaacaaat tgcatttgat ttagcatgca gaactataca gcagaatact ccacaaaaaa
     1201 atattgattt tattaatcta tataatgaaa taaggaagaa atacaacaac aacccatcgg
     1261 gacaacaaga gtacttaaac aaactcagag agctttatgc tacttatcaa aaaa
//
LOCUS       DAZFDC010000349         1310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFDC010000349 DAZFDC010000000
VERSION     DAZFDC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.352"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..343)
                     /locus_tag="ACHJ48_005112"
     CDS             complement(<1..343)
                     /locus_tag="ACHJ48_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710429.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLK"
     gene            complement(365..904)
                     /gene="yhaB"
                     /locus_tag="ACHJ48_005113"
     CDS             complement(365..904)
                     /gene="yhaB"
                     /locus_tag="ACHJ48_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO4710430.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAYFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1160..>1310)
                     /gene="tdcR"
                     /locus_tag="ACHJ48_005114"
     CDS             complement(1160..>1310)
                     /gene="tdcR"
                     /locus_tag="ACHJ48_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO4710431.1"
                     /translation="FSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCE
                     CNRFP"
BASE COUNT          409 a          224 c          241 g          436 t
ORIGIN      
        1 tttttaacgg ttcattaatg gtaagttcac aagtattgtc cgatcgttga ataatttggg
       61 cgttataacc taaaaaatcc agacgaagag acgagtcatt ataaagaatt acattttgta
      121 agtacgctaa ttctttttga aaatcaccag tgataatgca ttttccacta ggttgtaacg
      181 tgttaagtaa agtgaccatt tcaatagagg tctgttttag tttttcaata cctggtgcta
      241 gtgcttcgag gttatttcct gttgggcttt caggtaggtc attcattttt cgacggacta
      301 aatcactact aatatcatta ccaattgatg aaacagggaa cattatttta tcctttataa
      361 ataatcaata aaaatcatta ttaaaatgat cgggtgaaaa aaagagaatt tgccaacaat
      421 ctgaagtaat actggtttta ttaaagtacg cagggggtgt taagatggct tcttttcggg
      481 tgaattttgc acaatactta gaaatcgtct ccttaataca ttccagctct tttttattga
      541 cggctgagca agtacatttt tcgccaaaag gtgcaaaagt gcctacatct aatgtttgta
      601 tgatataatc gtcatgttct cctgtagaaa tatctatgat gaatggactc atattttccg
      661 gttggattcg gatcatgaca gaaggaacat cgccttgata gaaattcaat gcagtaattg
      721 cagataatac tctccgcagt tcattactta tactgtaaat tttattaacg tcattggtgg
      781 agacaatttc tgcaaacttt ctttttaccg tccaataatc aatatttcta ttgtgattgt
      841 taaccactgc gtgcattggt gggattgaag ggtgaaaaat atgtgcaatt gggaagcctt
      901 tcattgatgc ctgccttgtc atcatgacaa acaataatag atgcttattt tcctcgcagg
      961 gttctttcgc actatcaaaa aacgctcatt tacccggcgt tacagtaaac gaagcaacat
     1021 tctgtggcct acgccgatca aaagctctgt gaaaatttcc cttgttgtct ttgtctgacc
     1081 ttatcgcggc gatcagcaat tattcgtttt gtgatcctca acacaaaatg ctattcgtcg
     1141 cctatgaagg aagaagaatt tatggaaaac gattgcattc acaaaatgac gtcctgtcag
     1201 aagagaaaaa gttgacgtta atcaattgtt cgagatgatc aatacctgta ttcgaaattt
     1261 taaaatattg ataaaataaa tgtttgttct ccaacaaata gcgtgaaaaa
//
LOCUS       DAZFDC010000350         1309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFDC010000350 DAZFDC010000000
VERSION     DAZFDC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.353"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1170
                     /locus_tag="ACHJ48_005115"
     CDS             <1..1170
                     /locus_tag="ACHJ48_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO4710432.1"
                     /translation="MEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGL
                     GNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMT
                     LFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGI
                     TFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEK
                     YADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKRM
                     NKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIH
                     ASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT          246 a          319 c          358 g          386 t
ORIGIN      
        1 atggaagcct cgacctgggt cgcctttgct tttggcggta ttgtggcgat gttttccggt
       61 tatgcctatg cgcgtctggg ggcgagctat cccagcaatg gcggcattat cgacttcttt
      121 cgtcgcggat taggcaacgg cgtcttttcg ctggcgctct cgttactgta cctgttgacg
      181 ctggcggtga gcatcgccat ggtcgcccgt gcttttggcg cttatgccgt gcagtttttg
      241 catgaaggca gccaggagga gcaccttatt ttgctctacg cgttggggat cattgcggtg
      301 atgacgcttt tcaactcctt aagcaaccat gcggtagggc ggctggaggt gatcctcgtc
      361 ggcattaaaa tgatgatcct gttattgctg attattgccg gtgtctggtc gctgcaaccg
      421 gcgcatattt ccgtctctgc gccccccagc tccggtgcgt tcttctcctg tatcgggata
      481 accttccttg cctatgcggg ctttggcatg atggcgaacg cggcggataa agtgaaagat
      541 ccgcaggtca ttatgccacg ggcgtttctg gtggcaattg gcgttaccac gttgctttat
      601 atctcgctgg cgctggtttt gcttagcgat gtatcggcat tagagttaga aaaatatgcc
      661 gataccgccg tagcgcaggc tgcttctccg ctgctcgggc atgtgggtta tgtgatcgtc
      721 gtcatcggcg ctttactggc gacggcttca gccatcaacg cgaacctgtt cgccgtgttt
      781 aacatcatgg acaacatggg cagcgaacgc gaactgccga agcgaatgaa taaatccctg
      841 tggcggcaga gtacctgggg caacatcatt gtcgtggtgt tgattatgct gatgacggcg
      901 gcactaaatt taggctcact cgccagcgtt gccagcgcca cctttttgat ttgctatctg
      961 gcggtgtttg tggtggcgat ccgcctgcgt catgatattc acgcctcgtt gccgattctt
     1021 atcgtaggta cgttggtgat gttgttggtg atcgttggct ttatctacag cctgtggtcc
     1081 cagggcagcc gtgcgttgat atggattatt ggtgcaatct tactcagcct tattgtggca
     1141 atggtcatga agcgcaataa aaccgtataa cacctctctg cgcgcagtac tgcctgtact
     1201 gcgcctttgt ctttaattca aattaccttt cttattcctt taataaataa gtcacaaaaa
     1261 aatgatatat acgcgataaa tctgtgatgc taatcactta ttaccgtgg
//
LOCUS       DAZFDC010000351         1304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFDC010000351 DAZFDC010000000
VERSION     DAZFDC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.354"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(39..227)
                     /locus_tag="ACHJ48_005116"
                     /pseudo
     CDS             complement(39..227)
                     /locus_tag="ACHJ48_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          382 a          289 c          237 g          396 t
ORIGIN      
        1 aaccagcaaa cgggcattgt gttctgaaat ccaggctatt gattcaacgt cagcgacaga
       61 tctgcgctgt aaaagaactt gtaaaacaac gtaatagaat tatccggaat ggtggcgact
      121 atgcactaga acaccacaat ttcgctctct cggtaaggca ctgtcacagc attcaacaga
      181 atgtgacttg ccagatttat tagcaccacc agatgtttaa ccgggtaacc acgacccagt
      241 cggggatata ttccatcttc tcgctgacat catcaagaat aaaaaggttt attacactca
      301 gagcaacgtt cttcagcagg cgggataatt cttcaacatt tagaagatgt gttattaaag
      361 gcatacaaac tggacgtatt gtattttctt ttggtggttg acctaaggta gcagtttatc
      421 ctgatgcgct gagatttctg taatatctac gtcaacattc tcctcgaata gaaaatgcag
      481 ccccggcaag taattcacat tttttaacaa aacgttgtta gcgtgccatt ttcagaatct
      541 ctccaagaaa gcgaattctt ttctcaaatt ctgcattctc agacacagct ttctactcca
      601 tggtaatggc ccaaatacga agttgctcag gatcattagt ggtgcttaga agttaaataa
      661 ccatgcggag taccactcgt catcacgtat ttatatatca tgaagctatt gttgtgtttt
      721 gtacatcagt agatatattc tgatatactc cttttgctag acataacctt tcacctgctt
      781 gcaaagcttc tgtgttctga cattgccaaa ttgttgcaat tctgtatcca gccttctttc
      841 agtcatagct tcgggccgcg ataagactca ctgatctgac cctgattcct cttgcagact
      901 ttatagacca attaaaatgc agtttctgca ggtcaacgtc tgaccatcat tgtcatcact
      961 ctggccatta gagtaacctt ctgcattcat ccttttgtaa aaagtttata ttagtatcag
     1021 caattaaccg gacctgatac tgatatgagt cttaccgcat atacggtcaa tttcagcaat
     1081 taattacatt atccacgcca aagtatttgt catcacaatg atggtacctt ctttcagaca
     1141 ccattttttc aactccgttt tccacggacc gcactcttat gtcaagagtg cggtccgtgg
     1201 atacaaccag agaccgactg acacgagtca gaggaaacga cggatatgtt cagtcgtaaa
     1261 atatctatca aaaaacatga ttaaggtcaa aaatgtttga tatt
//
LOCUS       DAZFDC010000352         1275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFDC010000352 DAZFDC010000000
VERSION     DAZFDC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.355"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            400..>1275
                     /locus_tag="ACHJ48_005117"
     CDS             400..>1275
                     /locus_tag="ACHJ48_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710433.1"
                     /translation="MTIAKDSSTDEIIHGNDLRGMDDFYIKTTSFECPYEPCKIKATP
                     CSFTMKHVNQSYFRYGDKHKDGCGIHDPRYKNNHTSNDERKHNSPPAPVISLLKIDVK
                     PRGGVKNARNIKNENHKDEKKGNEHPVSSSSIKPVVDYYINNSNHNEQLSIPPYGTRS
                     YKDTFQLIFYKNNIRYYKPAIYYGVAQSNIRLHEDSDKHCITFLARDKKTQKPFTLEI
                     DVSDWNKSQKDVFWKEYEKQRKEADRYYKGLKDKRNAKKYLTVFFFGMPDENDKFLFK
                     TNHFKLVYVAFLGKFE"
BASE COUNT          459 a          192 c          220 g          404 t
ORIGIN      
        1 gtagtgtttg tatgattttc attagactta cttaatctat agtctaaatt ttaatattag
       61 attcgtaaat ctataattcc atgaatctaa ttaatgctta aaagcatcct ctaagatgct
      121 tttactgtta ccatgcatac atacccagaa cctttaacaa acgataatag aggggagttg
      181 tgtataacaa ctccctgacc ttactaaggt tagcgatgtt attcagcatc attctgtcaa
      241 agatttaatt tctttgaaaa atactaattc taccccttaa gttaataact tctgcttgat
      301 cgtgtatttt ggtaagttgg agtacaattt tttgattgga tcgtcaataa gaaaaagcta
      361 gaatccttag aaggacatag attttcatag ggttaagata tgacgattgc gaaggatagt
      421 tcgactgatg aaatcattca tggaaatgat ctccgtggaa tggatgattt ttacattaag
      481 acaacgagtt ttgaatgccc atacgagcct tgtaaaatta aagccacacc ttgtagtttt
      541 actatgaaac atgttaatca atcatatttc agatatggcg ataaacataa ggatggatgt
      601 ggtattcatg atccaagata taaaaacaat catacttcaa atgacgaaag aaaacataat
      661 tcgccacctg ctccggttat atcactttta aaaattgatg tgaaaccaag aggtggtgtt
      721 aaaaatgcca gaaatattaa aaatgaaaat cacaaggatg aaaaaaaagg taatgaacat
      781 cctgtatctt catcaagtat taagccagtc gttgactatt acataaataa tagcaatcat
      841 aatgaacagc tatcaatccc tccatatggc acgcgatctt ataaagatac atttcaactt
      901 attttttaca aaaacaacat acgttattac aaacctgcta tttattatgg tgtagcccag
      961 tctaatattc gtctgcatga agattcagat aaacattgta ttactttcct cgcaagggat
     1021 aagaaaaccc aaaaaccatt tacgcttgaa attgatgttt ctgactggaa taaaagtcag
     1081 aaggatgtgt tttggaagga atatgaaaag caaaggaagg aagctgatag gtattataag
     1141 gggcttaaag ataaaaggaa tgctaaaaaa tacttgacgg ttttcttttt tgggatgccg
     1201 gatgaaaatg ataagtttct atttaagaca aaccacttta aattagtata tgtggctttc
     1261 ttgggtaaat ttgaa
//
LOCUS       DAZFDC010000353         1273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFDC010000353 DAZFDC010000000
VERSION     DAZFDC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.356"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            161..1120
                     /locus_tag="ACHJ48_005118"
     CDS             161..1120
                     /locus_tag="ACHJ48_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO4710434.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          461 a          240 c          231 g          341 t
ORIGIN      
        1 aaacaccgtt acattaaaag ataataaaaa ccgcaataaa aaacgaataa atcaattgtc
       61 tcacgcaatt ataaaacatc atattgatta cgcaccttgc attacaaact catgtatgta
      121 aaatacgcgc accattcaaa aaaaaggaag acaataacat atgaaaaaaa gtgtcatcgc
      181 tggcgtcttt attgctctgt catttaccac gtgttcagct atcgcgaaca gccttgcatt
      241 atcattagca aatgatgatg cagggaagtt tcaaccaata cttaatgata tttatggcaa
      301 taaacatgaa aacagagatg attactcaca aggcttattt ctgggatata gccacgatat
      361 ctcagactcg agccaattat ctctccatat tgcgcaagat atttactctc catcaggcag
      421 taataaaaga cacaacacag ctgtaactgg agacagagct tttagtgcat acactcacac
      481 tggtattgaa tggaactccc ttgcgaatga ctggattcgc tatcgattag gtactgacat
      541 aggtgttgtt ggccccgacg caggcggtca gaaagtacaa aataaagctc atgagattat
      601 tggggcagaa aaatatcatg catgggatga tcaaatagag aatcgctacg gttatactgt
      661 aaaagggatg ctatccatga caccaagtat ggatatttta ggtgctaatg ttggattata
      721 ccctgaagtt tctgctgtta ctggaaactt atttcaatat gtagcatatg gcgcaaccat
      781 tgccattggt aatgataaaa ccttcaattc ggataatggc tttggtctgc tggctccccg
      841 tggtttaatg catatgtccg atacaagcgg attcaaatac aagatttttg caggtatgga
      901 aagacgagat gtcaatcgca actatactct cgaaggaaaa acaatacaga cgaaacaaac
      961 aacagtatcg ctaaacaaaa ctgttgatga atatcaagtt ggcgcaacaa ttgggtatgc
     1021 acctgtagcc ttcacactag catttaataa agtaacatca gaattcaaga caggggatga
     1081 ctattcattt ataaatggag caatcacctt ctttttttaa ctgaattgaa ttcaatcaaa
     1141 ataacataag tccaacaaaa acataaagtg cgaaatgaat gccagctcca tttatttcgc
     1201 actataaaag attaaaagtt gcaataaaat aataaaatga ctcagttacg aaaaccaata
     1261 aactgtggcc agt
//
LOCUS       DAZFDC010000354         1268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFDC010000354 DAZFDC010000000
VERSION     DAZFDC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.357"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..633
                     /locus_tag="ACHJ48_005119"
     CDS             52..633
                     /locus_tag="ACHJ48_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4710435.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            976..>1268
                     /locus_tag="ACHJ48_005120"
     CDS             976..>1268
                     /locus_tag="ACHJ48_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710436.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKA"
BASE COUNT          305 a          295 c          402 g          266 t
ORIGIN      
        1 atctgccttc acggggattt acaacgattt ggccgccgcc tcagcctgta tgtgaacacg
       61 gcagcggaag ccatccgggc gctgtcgtta caggtgccgg gctttcgccg tcagatgaac
      121 gaaggctggt accagatacg tattgccggt tatgacacgg caccggaggc ggtgtacgcc
      181 cgtcttcacg aacagctggg tgagggaacg gtcatccata ttgtgccgcg actggccggg
      241 gccggaaagg gtggactgca gattgtgctg ggggcggcag ccatcgtggg ctctttcttc
      301 actgccgggg catcaatggc gttatggggt tcagccctgg cagccggtgg tttttctgcc
      361 accacgatgc tgttttcact tggagccagc atgattctgg gcggtgtggc ccagatgctg
      421 gccccgaagg caaaaacacc ggattaccgc gcaacggata acggcagaca gaacacgtac
      481 ttttcctcgc tggataacat gattgcccag gggaacccga tgccggtgcc ttacggggaa
      541 atgctggttg gctcccgccg tatatcccag gacatcagca cccgtgatga aggcgggggc
      601 ggaacggtcg tggttgtcgg gcgacaggga taaaacataa aaaaatcccg cagtgatcgc
      661 ggagctgcgg ggacagacaa atgaagatca atgttaagga gttgtttttg ttactcgggc
      721 aaaaaaacac taacgcagcg aaattataag cgccacagtc agtgtgtgaa aatgtgaaga
      781 tattcagaat ttttatgcca ttaccggttt taaccaacag gattatcggt gggcatgaaa
      841 gaaaaccccg gtatctgctg ataccggggt ttctctttag catggcagaa atgtgtttca
      901 tgcttttcgg gcgaaggata tccgacttct gtacggaatg gcaagtggcg gttaatttat
      961 tcaggggaag gctgtatggg aaaaggtggc ggtaaggcac acacgcctcg tgaggcgaag
     1021 gataatctca aatccacgca gatgatgagt gtgattgatg cgattggtga gggaccgata
     1081 gaaggtccgg tgaagggact gcagagtatt ctggtgaaca aaaccccact gacggacacg
     1141 gacggcaatc ccgtgataca cggtgtgacc gcggtctggc gtgccgggga gcaggagcag
     1201 acaccaccgg aaggctttga gtcctccgga gctgaaaccg gactgggcgt ggaagtgacg
     1261 aaggcaaa
//
LOCUS       DAZFDC010000355         1263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFDC010000355 DAZFDC010000000
VERSION     DAZFDC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.358"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1200)
                     /locus_tag="ACHJ48_005121"
     CDS             complement(<1..1200)
                     /locus_tag="ACHJ48_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFO4710437.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDSSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTE"
BASE COUNT          351 a          275 c          241 g          396 t
ORIGIN      
        1 ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga tatctttgtt
       61 tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg catttagttc
      121 gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt gttgtaataa
      181 cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt catcgtttgc
      241 taattcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga tgataaattg
      301 gcgcaccgct gagggatcaa tagattcagc aatcaatgac cactcaccac taccgagttg
      361 ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga tataatcttt
      421 gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg caacgtcttc
      481 cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa ccggaaattt
      541 cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta ctttgttcat
      601 tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt tagacaatga
      661 actactatca ccattaaaag taaactggcc ctcaccaaaa gaaaactttc catactctgc
      721 aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca atgtcgcttc
      781 cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat tattcactaa
      841 gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca ttgtgatggg
      901 caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct tgatattgag
      961 atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa aaaagccttt
     1021 gtcaaaattt tgataactta acgtcatatc gtaggcatgt tcacctttct ttttagcatc
     1081 gttaaaatct ttaataaatt tttcgacacc aggctgaatt tgcgtacctg tgtaccatgt
     1141 gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact tacgtatcat
     1201 ataaatattc cctgataaaa cagcaaaact ttgaatatcc acttatgctg aaataatgtc
     1261 cag
//
LOCUS       DAZFDC010000356         1255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFDC010000356 DAZFDC010000000
VERSION     DAZFDC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.359"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(117..1217)
                     /gene="ompC"
                     /locus_tag="ACHJ48_005122"
     CDS             complement(117..1217)
                     /gene="ompC"
                     /locus_tag="ACHJ48_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO4710438.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRDNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGIDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          356 a          277 c          262 g          360 t
ORIGIN      
        1 tattaattga ggtgaatgac cagaaatatc aaaaattgct catttttact taaggagtca
       61 tacagcagca cttaaatcaa aaaccagccc agagaagggc tggtcaggag aggatgttag
      121 aactgatagg taatgcccgt agcgacgatg ttatcggtgc gaatatctac ggcacgggta
      181 aagtcgcttt catcaatcag gttgatttta taatcaacat aggtcgacat atttttgttg
      241 aagaaatacg tcgcaccaac gtcgatatac tcaatcatgt cctgatcgcc gtagcggccc
      301 agatctatgc ctttcgattt caggtaagcg agtgaggggc gcaagccgaa gtcgaattga
      361 tattgcgcca ccacttcaaa actttgtgct ttgttagaaa tataaccatc gccataggtg
      421 gtcatgttta atgtttcagc gtaattagct gcaaagtaga tgttgttggc atcatatttt
      481 atacctgtag cccacagttc tgcattctta ccggaggcat tgagaggatt agcagcggct
      541 ctttcctgcg catttgtccg atcggatttg gtatacaccg ctccgatacc aaagccatca
      601 taaacataag ttgaggagat accgaaaccg tcaccgttgg cacgcgtgta gtcagcacca
      661 taaagatgac tgttgtcagt acgttcattt ttgccttgat attgcgcggc aaagttcagc
      721 ccttcaatta agccaaagaa atcgtagttg cgataggttg ctacaccggt ggtacggtat
      781 gtcatgaaga catctttctg actccaggta tcgccaccaa attctggcaa catatcagtc
      841 catgaaccaa tgtcatagcc gacaccgtag ttacggccat agtccagaga gccaaattca
      901 ctaattttta aaccagcata ggctagacgg gtcttgttgt cgcgcgagcc gttggcttca
      961 tcgttatagc ctttaaaatc atattcccag tgaccaaaac caatcatttg atcattgatt
     1021 tgcgtttctc ctttaaagcc gagacgggca taagttttat caccatcgtc acgcttatca
     1081 tcagtaaaat aacgtagagc ggtaaccttg ccataaaggt cgagtttatt accatccttg
     1141 ttatagactt cggcagcgtg tgctacgttc gcagctaaca aaccagttac aaccaccgca
     1201 actattttta atttcattta acagaatcct tttaattatc gttaaacgta ttttt
//
LOCUS       DAZFDC010000357         1246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFDC010000357 DAZFDC010000000
VERSION     DAZFDC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.360"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..490)
                     /gene="ybfC"
                     /locus_tag="ACHJ48_005123"
     CDS             complement(<1..490)
                     /gene="ybfC"
                     /locus_tag="ACHJ48_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO4710439.1"
                     /translation="MIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFPVLNNKAMWR
                     WYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGTLQELINTAE
                     KTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYARMTAHLPNK
                     DES"
     gene            complement(515..>1246)
                     /locus_tag="ACHJ48_005124"
     CDS             complement(515..>1246)
                     /locus_tag="ACHJ48_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4710440.1"
                     /translation="LISKEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGG
                     YGARPNKPPTPDPSKFPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKW
                     VKPNLTCTPQDDPQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGP
                     ATAGIDDLGEILAKMKQRTSRGIRK"
BASE COUNT          315 a          287 c          244 g          400 t
ORIGIN      
        1 aagactcatc tttgttcggg agatgtgctg tcattcgagc ataagttggc ttctcatctt
       61 taaaatattt aaccatgatt gaattatcaa ggactgccaa caactgagct ggatcactgc
      121 tctttttttg atagataccc gctcttatat aacttctaaa ccgatcgccg agaaacgccg
      181 ttttttctgc tgtatttatc aactcctgca acgttccttt gatggcatga ttttcattaa
      241 ggcgtagtga cccgacacga attaagagtc tcgccccgct tggtgtgaat ttattatttt
      301 tacatgtacc cagttctgtt tgccaataat actcaccgat gtcctcattc tgataccatc
      361 tccacatggc tttattattt aagacaggaa acgcggaaaa aatagtaccc ttagcgaaaa
      421 tagtaaaaca atcatgatct ttagcttgta tttcgcaatc agctataaca ccaaaactaa
      481 caaatatcat cagcaaaaaa aaacaaaact cttttcattt tcttattcct ctcgatgttc
      541 tctgtttcat tttagctaaa atttcaccca aatcatctat ccctgctgtt gctggcccat
      601 cgggtttatt aatgtcaggg aataaatagg gttctgcaca agtatatcct ggtggtaatt
      661 gagttgttgg ccaatttgtc tgtggcaggt aatcacttgc tgtctttgta tccatccctg
      721 gatactgggg atcatcctgt ggcgtacaag tcagatttgg ttttacccac ttcctgcaat
      781 acagtgtgta gtcgtaaggg cttaatgctc tgaagaatgt tttgaaaaca ttaggcgcac
      841 tactggcctg gtcaatagga tatggcagtc ttaattgttt cgctatgtct ggaaatttac
      901 ttggatcggg cgtaggcggt ttgttgggtc tcgctccata tccgccatta tttaagtgtc
      961 catattttga tgccagtccc attgaatcta tatactgaac cggattcaac ggatactgat
     1021 aaaaattcca tccccccttc agtccaatcg gatcctgagt gatatacctc ccctgcagcg
     1081 ggtcataata gcggtggcgg ttgtaataca ggccggactc ctcatcatac tgctgccccg
     1141 gcaggcggat aagctgctgc agctgatgcg ggttctcttc attcagcagg ttgccccact
     1201 catcgtattc tgcgcaccat tctgttgccc cttccttact gataag
//
LOCUS       DAZFDC010000358         1238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFDC010000358 DAZFDC010000000
VERSION     DAZFDC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.361"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1226)
                     /locus_tag="ACHJ48_005125"
     CDS             complement(<1..1226)
                     /locus_tag="ACHJ48_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO4710441.1"
                     /translation="MKVFHNIFKYISSNHQDKHSDKVNNHQHHGKVDKKHRSEIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKIWFYNSEPSIDIKEYNSFADNT
                     TDTFIFTIIPDNNHVIKLSSPITVTIERKDGYYFINFSGDKSDIIYKVDGFSIKERNF
                     FTLLSGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYALLVSGHIGIREKGWSLLCDLLKHEDMASFAYENKKLKKLFTLLDKRD
                     MILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVVVLAGNHEINFNGNYSARLANHKLSSGDTYNLIKTLDVCNYDSERHVLTSHHGI
                     IRDEENKCYCLGALQVP"
BASE COUNT          395 a          233 c          192 g          418 t
ORIGIN      
        1 ggaacctgca aagcaccgag acaataacat ttattttctt catcccttat tattccgtga
       61 tgactggtta aaacatgcct ttctgagtca taattacaaa catcgagcgt tttaattaag
      121 ttatatgtat caccggaaga gagtttatga tttgctagtc gtgcactata gttaccatta
      181 aaattgattt catgatttcc agccagaaca acgacatttt tatttatacg actatcttta
      241 ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc accaaaaata
      301 gtactgaatc tatcgcctgt atgatcccca aggaagatac aaggggtaat cgcatcgcct
      361 tttaaaaaaa catgttgatg caactcattc aagatcatgt ctctcttatc taataaagtg
      421 aagagttttt tcaatttttt attttcatat gcgaatgaag ccatgtcttc gtgtttcaat
      481 aaatcacata ataaactcca ccccttttcc ctgatgccta tatgccctga aacgagcaat
      541 gcataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt gttggcattt
      601 tgtgggttaa tcacaccaca ctgtttataa aagtctattt ttgaaaaaac agattcaaca
      661 tagctatcaa acttttcctt agtaaacgtc tctttagaga cagcccaacg ccagtcaggt
      721 ttgaaattac cactaagaag agtaaagaaa tttctctctt tgatagaaaa cccatcaact
      781 ttataaatta tgtctgattt atccccggag aaattaataa aataataacc gtccttacgc
      841 tcaatcgtaa cggtaatggg agacgataat tttatcacat ggttattgtc tggtataatt
      901 gtaaagataa acgtgtcagt agtattgtcg gcgaaactat tatattcttt aatatctata
      961 gatggctcac tattgtaaaa ccatattttc tctatctgag gactatgttc agttacgttc
     1021 cagcagccat gttgcaggaa atagatgata tgcaggccaa aatcgttatc tatttgcgaa
     1081 tgtttatcca gcttatcaat ttcaactatc tctgaacgat gttttttatc aaccttgcca
     1141 tggtgttgat gattattaac tttatctgaa tgcttatctt gatggtttga agagatgtac
     1201 ttaaatatat tgtgaaatac tttcataaat aacccgtg
//
LOCUS       DAZFDC010000359         1233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFDC010000359 DAZFDC010000000
VERSION     DAZFDC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.362"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..676
                     /locus_tag="ACHJ48_005126"
     CDS             <1..676
                     /locus_tag="ACHJ48_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710442.1"
                     /translation="IESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAG
                     RINARLEDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVC
                     RWFGTSDLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEI
                     DAFFCAFREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALRE
                     ELETML"
     gene            673..>1233
                     /locus_tag="ACHJ48_005127"
     CDS             673..>1233
                     /locus_tag="ACHJ48_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO4710443.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGA"
BASE COUNT          309 a          305 c          357 g          262 t
ORIGIN      
        1 aatcgaatcg accgtgacca gcctgcaccg tgaggctgaa gcacagttcc ggcctgagct
       61 ggagaaaatc gtcagcggga ttgaaacagg ttttcgtggc acggccctgt acgccacaga
      121 aaacattgcc ggtcgcatca atgcccgcct ggaggatgag ggctttaccg taaaaatcag
      181 ttttccggca gtcagccagt tacagacccg gctcgcggta aaaataaatc tgagtgcgct
      241 tatggaggaa agaacggaga ccgtcacccg tcgccgtcgg cagagtggcg tatggggaac
      301 cgtttgtcga tggtttggca ccagtgacct gggctgggaa aactatgacg aggatgtgag
      361 tcgcagcgtg atcaatatca acaaggtcag agaggaagtt atgtcactga cccgggcata
      421 tttcggggag ctgcaggcat ccattgagca ggatattaac cagcccgtcc gccaggaaat
      481 cgatgccttt ttctgcgcat tcagggagaa agttgaacaa ctgcgtaaca cgctgattca
      541 gagctctgaa gatcataaac gcgatcagca ggcgcaggaa cggcttaccg ggcgacttca
      601 ggcattaaat gaaagggttc ctgagctcat tactgacagt aaggcgctga gggaagagct
      661 ggagacaatg ctgtgacttc accatttatt caacaaattg ctgataaccg ggtatgtcag
      721 gtactcacct gtcttcctga aaaatttgtg gttgatttcg caaacggaat cgatgttgca
      781 caggagcata tccgcacggc cggagagcgc acgttcttcc ggcgtttaaa agaggggctg
      841 accggcgaag gcgctgcacg gcagaatgcc atcaatgcgt cgcttgcaca gggcgttgag
      901 gcgtccctgc gctggctgac ggagatgaca acgtcacttg ccaccacaaa ctacgcgatt
      961 acccgggtaa acgacagggt cagttcactg gtcagtgata cagccaggct ggcgcattat
     1021 tccgcagata cgcgggagca gttactcacc ctggccgatc aggttcacca taaactgaat
     1081 catcttgaag aaaaactcca ccgtgttgac caggttcagc gggcgcagtt acatcttgag
     1141 cagatattct catggtggag cgccgggcga tatgcgtcat tttcccctgc cggacgttgt
     1201 tatgtggcac ttgaagagct tcgctggggc gcg
//
LOCUS       DAZFDC010000360         1226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFDC010000360 DAZFDC010000000
VERSION     DAZFDC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.363"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHJ48_005128"
     CDS             complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHJ48_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO4710444.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVR"
     gene            704..>1226
                     /locus_tag="ACHJ48_005129"
     CDS             704..>1226
                     /locus_tag="ACHJ48_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO4710445.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNE"
BASE COUNT          373 a          252 c          229 g          372 t
ORIGIN      
        1 tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc actatgacgg ttaaacagat
       61 gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt
      121 cggcacgtaa gaggctaacc tagatttgcc gttcatagat tgagcctcag attaatgtta
      181 ggcgtcttgc aggacgcgta atgttaactg gggcttttct ctatctgccg ttggtgttca
      241 tgcccgaggc agatagcctc aagcacccgc agctattcta cttaacttcc cattacctcg
      301 ccaatatgaa atcggtcaga aagacagacg tcccataagg acaacaacgg aaaaagagat
      361 tttgcttgat gctatttacc tctgcaccat gaagttgcgc caaaatccca gtggtgataa
      421 caggaatttg gttgttggtg attggggaaa gaagtacgtt agactgtggg ttagttcatt
      481 gacatgatag ttacctgcct tcaatttcct aagcaagcga tgtaaacgat aatcaatatc
      541 aaatgtattg acaggtaaaa taataacgat gtacctttgc tgataacata tatcagtctt
      601 aaatcataat ggaggtagat aaaataacta aacacacaca acaaattttt tatcagtgac
      661 atagcatatc ttaagcatcc tttaatgctc atggggagaa cgcatgacta aacatatgga
      721 cattcattgt caaacacgca tatttaactc aattaaccat gacaccaaat ataaaatatg
      781 tatttttact ccaaaaaaca ttaaaaaaaa taatgcaact ctttatatac ttgatgggaa
      841 tagtgccaac aattacattt ctgatattct gcctgttatt gatgcactac ccaatccacc
      901 agtgttagtt acacttggtt atgaatcttg gaataacctt agcattcatc gccgtgctta
      961 cgattatact cctgatggtg aaaatgctat tgttgataac tctaaaccag catggatcta
     1021 ctttactggt ggaggaagcc agtctttccg agaattatta ctaactcaga tcatgccatg
     1081 ggttagtact attgctccga acagttccag aatcggtata tggggacact cgctaggtgc
     1141 tatttttgtg cttgattgct taaaaaataa ttcatgcttt aactattatt atatatctgc
     1201 accatcactt ctatggcaga atgaaa
//
LOCUS       DAZFDC010000361         1193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFDC010000361 DAZFDC010000000
VERSION     DAZFDC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.364"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1193
                     /locus_tag="ACHJ48_005130"
     CDS             <1..>1193
                     /locus_tag="ACHJ48_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO4710446.1"
                     /translation="PLVESRYLYDPLGRRTGKRVWRRGRDLTGWMSLSRKPEVTWYGW
                     DGDRLTTVQTDTTRIQTVYEPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGH
                     GVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHL
                     YHCDHRGLPLALISEDGNTAWSGEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLY
                     YNRNRYYDPLQGRYITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQL
                     NHQRAWDILSDTYEDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIR
                     DYGLNLFGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKE
                     TIKAL"
BASE COUNT          320 a          259 c          385 g          229 t
ORIGIN      
        1 gccactggtc gagagccgct acctctacga cccgctgggc cgccggacgg ggaaacgggt
       61 gtggcgtcgc gggcgtgacc tgacgggatg gatgtcgctg tcgcggaaac cggaggtgac
      121 gtggtacggg tgggacggcg accggctgac gacggtacag accgacacca cgcgtatcca
      181 gacggtatac gagccgggaa gcttcgcgcc gctcatccgc atcgaaacag acaacggtga
      241 gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg cagcaggaag ggagcgagga
      301 cgggcacggt gtggtatttc cggctgaact ggtgcggctg ctggacagac tggaggaaga
      361 aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg cttgcgcagt gcgggctgac
      421 ggtggagcaa ctggccagac aggtggagcc ggaatacaca ccggcgagaa aagttcatct
      481 ttaccactgc gaccaccggg gcctgccgct ggcgctcatc agcgaagacg gcaatacggc
      541 gtggagcggg gagtatgatg aatggggcaa ccagctgaat gaggagaacc cgcatcacct
      601 gcaccagccg taccggctgc cggggcagca gtatgataag gagtcggggc tgtactacaa
      661 ccggaaccgg tactacgatc cgttgcaggg gcggtatatc actcaggacc cgatagggct
      721 ggagggggga tggagtctgt atgcgtatcc gctgaatccg gtgaatggta ttgatccatt
      781 agggttaagt cccgcagatg tagcgctaat aagaagaaaa gatcaactaa accatcaaag
      841 agcatgggat atattatctg atacttatga agatatgaag agattaaatt taggtgggac
      901 tgatcaattt ttccattgta tggcattttg tcgagtgtct aaattaaatg acgctggtgt
      961 tagccgatcg gcgaaagggc tgggttatga aaaagagatt agagattacg ggttaaatct
     1021 gttcggtatg tacggcagaa aagtaaagct atcccattct gaaatgattg aagataataa
     1081 aaaagacttg gctgtaaatg accatgggtt gacatgtcca tcaacaacag attgctcaga
     1141 tagatgtagt gattatatta atccagagca taaagaaacg ataaaggctt tac
//
LOCUS       DAZFDC010000362         1169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFDC010000362 DAZFDC010000000
VERSION     DAZFDC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.365"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            38..562
                     /gene="lpfA1"
                     /locus_tag="ACHJ48_005131"
     CDS             38..562
                     /gene="lpfA1"
                     /locus_tag="ACHJ48_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFO4710447.1"
                     /translation="MKKVVFALTALALTSGTVFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADKSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            627..>1169
                     /locus_tag="ACHJ48_005132"
     CDS             627..>1169
                     /locus_tag="ACHJ48_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO4710448.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRFTRNGNQL
                     RVDNPTVFNITFNQFFANDKE"
BASE COUNT          317 a          244 c          274 g          334 t
ORIGIN      
        1 ttttcttttt aatgtaattt tttaaatgga gtttttcatg aaaaaggtcg tttttgcctt
       61 aaccgcactg gcgttaactt ctggcactgt attcgcagca gagtctggtg acggcacggt
      121 taaatttaca ggcgagatcg tggattcacc ttgcgtactg tccgttgact ctcagaacca
      181 ggaagttgtg ttgggtcagg tacaaaaatc tgtatttgct gcggttggtg acaaatcccc
      241 ggcaaaacct ttcgaaatca aactggaaga ttgcgatacc accacgatga aaaaagccaa
      301 tgtgagcttt agtggtgttg gtgatgcaga taaatctgat ctgatttctg ttagcactga
      361 agcgggcgcg gccaaaggtg ttggtatcgg tatttatgat aacagcaata ccctggtcgc
      421 tcttaacggt ggtaaagcaa gtgttgatct gagtaaaggc cagacggttc tctactttac
      481 cgctaactac gtttctactc tggcaacggt aacgaccggt tacggcaatg cgcaggtcga
      541 tttcaacctg tcttacgagt aatcgtccgt tttacaaatg gcggcgtaat tgccgccatt
      601 agtcgtcaaa gttcatggat cgaatgatga aaagtaaatt tgttgcgttg gctctctctc
      661 tgttcctttc acagtcagtt ctggcgggtg gcgtcggact tagctctact cgcgtaattt
      721 atgatggcag taagaaagaa gcctcgctga ccgtacagaa taaaagtaag acggaagagt
      781 ttcttatcca atcctgggtg gatgatgccg cgggcagcaa aaaaacaccg tttattatta
      841 cgcccccact attcaagctt gatccagaaa agaacaatat tttgcgtatt gtgaatatta
      901 cgcccggcct gcctcaggat cgcgaatcgg tctactgggt taatgtgaaa gccattccat
      961 ctaaaagtga cgacagcgaa aacaaaaatg ttttgcagat tgccgttcgc acccgaatca
     1021 aactttttta tcgcccggca ggtttaaagg gtgatgtaaa aaccgcgcca aatgaactgc
     1081 gatttacgcg taacggtaat cagctccgcg ttgataatcc gacagttttt aatattacct
     1141 ttaatcaatt ttttgcgaat gacaaagaa
//
LOCUS       DAZFDC010000363         1166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFDC010000363 DAZFDC010000000
VERSION     DAZFDC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.366"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            60..347
                     /locus_tag="ACHJ48_005133"
                     /pseudo
     CDS             60..347
                     /locus_tag="ACHJ48_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            791..>1166
                     /gene="espR1"
                     /locus_tag="ACHJ48_005134"
     CDS             791..>1166
                     /gene="espR1"
                     /locus_tag="ACHJ48_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HFO4710449.1"
                     /translation="MKFPSIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLSTQPPGLQNFTSINLDNNQLTHFDATNY
                     DRLVKLSLNSNTLESINIHQGRN"
BASE COUNT          433 a          237 c          175 g          321 t
ORIGIN      
        1 tagcatcgct ggatgtctcc cggcaagagt ctctgattga gttaagtgcc catcacaata
       61 tgatcactga ccttatatta cacaatcatc ccaggatgaa aacaatcact ttaaacgaca
      121 accatattgc acatttaaac accaaaacta ctacaaaact tgaatattta aacttaagca
      181 ataacaattt attgccaacc aatgacattg atcaactaat atcatcaaaa catctttggc
      241 atgtattagt taacggcatc aacaatgatt cacttgcaca aatgcagtac tggactgcag
      301 taagaaatat aattgatgac actaatgaag tgaccattga tttatcttat aatctggcaa
      361 tcacaaatat cgataccagt gatgaacatc ttgtagaagt aagcgataat tcagaaggta
      421 attatataaa agacaatgac tcaatgtcta ttcgttatag atcaaaatat tattccagag
      481 attccgcctt aatagaagaa gaaacaatat tttctgacgc agaactaaaa gctattctgc
      541 ctatgcgtcg catgtacggg gttagtgact ataagtcaaa ttcctcttct ctatcctcac
      601 actcgggact caaggaccca acgggcacac ccgtctgtta ttatattcat aatgagagta
      661 aaccttcctt aggttatggt ccaacatcca ataattggtt aagccaatcc tttacaacag
      721 agttataaaa cacatcctgg tttatcaagg gtgataaatc agaaaaacat tgtattacta
      781 tggagcagtt atgaaattcc cttcaatatt taacaaaata aaaccacaat ccatacagca
      841 acatccagaa aaaaatcaac ttaactggat gctcgaatta aataaatgga aagaagaacg
      901 tatacttaca ggtgaaatcc atcgtccgga atgtcgaaac gaagccgcta aaaggataaa
      961 ctgtgctttt ttgtcgaaac agaatgacat tgatttatca ggacttaatt tatctactca
     1021 accaccaggg ctgcaaaact tcacctctat caatcttgat aataaccaac tcacacattt
     1081 tgatgcaacc aactacgata gactcgtaaa acttagtctg aatagtaaca ctcttgagtc
     1141 aataaatatt catcaaggca gaaatg
//
LOCUS       DAZFDC010000364         1166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFDC010000364 DAZFDC010000000
VERSION     DAZFDC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.367"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..138
                     /locus_tag="ACHJ48_005135"
                     /pseudo
     CDS             1..138
                     /locus_tag="ACHJ48_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154256.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
     gene            639..1139
                     /locus_tag="ACHJ48_005136"
                     /pseudo
     CDS             639..1139
                     /locus_tag="ACHJ48_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          334 a          223 c          247 g          362 t
ORIGIN      
        1 ttaacagcta tccgtatggc gtgttcggtt ttgcgaataa aaaatggtta ctgtagaagc
       61 ggatttccac cacatgtcaa ccatgaatgg tcagtgatgc gagacgactg ggatgatttg
      121 gtcgatgact tcttccggcg aatactgata gaaacattga aatattgtaa gagaaagcgt
      181 tcaaaattct gtggcacgtg aaaaattcta cagcgtcatc accttattca gtcgaccttc
      241 aaaataaacg tttaacagag acaatcacca tttggattgt ccgctgagga ggcgaacaat
      301 ttcctagaca gctttatcat gtcagcccac attgttggcg ctggcaatac taaggacaaa
      361 cgatggggaa gtagcttttt agcgttattt tcgggagtaa aaaacgtggt atctttccgt
      421 atgtcagttg tatcttgctg gtattcgtag tcgtgataag gcatacaacg atatttatct
      481 tgacgttagt gaagaataag ttgttttgtg atctatgtta atctgggatt acaatccggg
      541 cttgatacat cgcatcacga cttttattct cttcagaata gaatatgtta ctccccgctt
      601 tacaggctca ggatttgaat tacaaattat acttttaagt gaacaataac caagggtatt
      661 tcttatttcc tgaaaccagg cttggcccat ttcgggatga gtttgaaaaa actcttgacg
      721 catacgatag tgatgaaata aacgaaacaa attacgtcaa caaactcagg cgtcttgcct
      781 gacatgctcc tgacatcatt gatattcacg ctcatctggc atatgcattt gcgctcttta
      841 tgctgcaatt ctggcgaatg ttcatttaca gcgtcatcag gatgccgtta tactcattga
      901 taaaatacta acatataacc ctgagaataa tcagggaaca cgctggttac tgggggcgga
      961 attgttacgt gctggcaagc atggcagggc tctgtacgta ttgacaggac atgtcaatga
     1021 atttcccccc tactggtacg agcttgctct tttacatttt atgaatgagg catatattaa
     1081 tgctgcaacg gcatttcgat atggattttt aacaagtata tattgttgaa atgctttgtt
     1141 agaatcttta tccattccct agggct
//
LOCUS       DAZFDC010000365         1148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFDC010000365 DAZFDC010000000
VERSION     DAZFDC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.368"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..441
                     /locus_tag="ACHJ48_005137"
     CDS             <1..441
                     /locus_tag="ACHJ48_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4710450.1"
                     /translation="DEKEGNHTTAYRDGAGIWTICRGAILVDGKPVVPGMKLSKEKCD
                     QVNAIERDKALEWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            597..779
                     /locus_tag="ACHJ48_005138"
     CDS             597..779
                     /locus_tag="ACHJ48_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710451.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            792..923
                     /locus_tag="ACHJ48_005139"
     CDS             792..923
                     /locus_tag="ACHJ48_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710452.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            931..>1148
                     /locus_tag="ACHJ48_005140"
     CDS             931..>1148
                     /locus_tag="ACHJ48_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO4710453.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLANVTITDMQVRQRDVAALDARYSRELA"
BASE COUNT          288 a          237 c          341 g          282 t
ORIGIN      
        1 gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat ctggaccatc
       61 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      121 gaaaaatgcg accaggttaa cgccattgaa cgtgataagg cgctggagtg ggtggagcgc
      181 aatattaaag taccgctgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
      241 aacattggtc ccggtaagtg tttcccgtcg acgttttaca gacgaattaa tgcaggtgat
      301 cgcaggggag cgtgtgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      361 attcgctcaa acaactgcta cggtcaggtt tcacggcgtg accaggagag cgcgctggcg
      421 tgctggggaa ttgacagata agcagaatat tttgctgaaa aatgcggttt gctcacactg
      481 gcggataaca cgaaatcctg cgaactggca aaaactaagt gaataaaagt aaaaccccgt
      541 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatgg
      601 gaaagtataa acgaattctg ttgagattga ctatgaaaaa cggccttgaa ctgaaagcgc
      661 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
      721 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
      781 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
      841 gcatttcatt ttcactaatg ctggttgcta tttgcttttc cactgcctgg cgcgtcttaa
      901 ctttgtcatg gctggtgggg tgagggggat atgaaccgtg ttctgtgtgt ggtgattatt
      961 gtcctgctgg tagcctgtgg tgtgcttagt ctggggctga atcattaccg cgataacgcc
     1021 attacctaca aagcgcaacg cgataaaaaa gccagtgagc tgaagctggc gaacgtgaca
     1081 attactgata tgcaggtacg ccagcgtgat gtcgctgcac ttgatgccag atactcgagg
     1141 gaattagc
//
LOCUS       DAZFDC010000366         1147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFDC010000366 DAZFDC010000000
VERSION     DAZFDC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.369"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..220
                     /locus_tag="ACHJ48_005141"
     CDS             <1..220
                     /locus_tag="ACHJ48_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO4710454.1"
                     /translation="PENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTINGEMFW
                     VDRVSPDDGGSCYLWLNRGQPPAVNRRR"
     gene            235..768
                     /locus_tag="ACHJ48_005142"
     CDS             235..768
                     /locus_tag="ACHJ48_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4710455.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            765..>1147
                     /locus_tag="ACHJ48_005143"
     CDS             765..>1147
                     /locus_tag="ACHJ48_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO4710456.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAVTAIPALADLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          274 a          269 c          348 g          256 t
ORIGIN      
        1 ccctgaaaat atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct
       61 gtttgtccgg actgatgatg tgcggcaact gcggcgtggt gacacgctga ccattaacgg
      121 cgagatgttc tgggtggacc gtgtttctcc ggatgacgga gggagttgtt atctctggct
      181 caaccgtggg caaccaccgg cagttaaccg gcgacgataa acgcagggtg aattatggcg
      241 ataaaagggc ttgatcaggc gattgaaaat ctgagccggg ttcgtaaaaa cgccattccg
      301 gcggcttcag caatggccat taaccgcgtg gccacaacgg cgattaatca gtcttcatca
      361 caggttgccc gggagacaaa ggtacgccgg aaactggtaa aggaacggtc cagactgaaa
      421 cgggccacgg tcagaaatcc gaatgccaga attatcgtta aacgcggtga tctccctgtg
      481 attaagctgg ggatcaggat gctgggccgt cgtccggaca gcatacttaa agctggtcag
      541 catcggtatc agcgggcatt tatccagcga ttaaaaaatg gtcgctggca tgtcatgcag
      601 cgtgtggccg ggaaaaaccg ttaccccatt gatgtggtga aaatcccgat ggcggcccca
      661 ctgaaacagg cgtttgatga gaatattgac cgtatccggc gtgaacgtct gcccggagaa
      721 ctggcatacg cgctgaaaca acaacttagg attgcgataa aacgatgaaa catactgata
      781 tccgtgcggc agtgctggat gcactggagc tgcatgaaca cggggcgacg ctgtttgatg
      841 gtcgccccgt tgtttttgac gaagaggatt ttccggccgt cgcggtttat ctgacggatg
      901 cagagtatac cggtgaagag ctggatgcag atacctggcg ggccacactg catattgagg
      961 tgtttttacc ggcacaggta ccggattcgg agcttgatca gtggatggaa agccggattt
     1021 atccggcggt gactgcgatc ccggcactgg cagacctgat tacgacgatg gttacgcagg
     1081 gctatgagta tcgtcgtgat gacgatatgg cgttatggag ttctgcggat ctgacttatt
     1141 ccattac
//
LOCUS       DAZFDC010000367         1147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFDC010000367 DAZFDC010000000
VERSION     DAZFDC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.370"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..164)
                     /locus_tag="ACHJ48_005144"
     CDS             complement(<1..164)
                     /locus_tag="ACHJ48_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO4710457.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLAD"
     gene            complement(389..943)
                     /locus_tag="ACHJ48_005145"
     CDS             complement(389..943)
                     /locus_tag="ACHJ48_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4710458.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(1006..>1147)
                     /locus_tag="ACHJ48_005146"
     CDS             complement(1006..>1147)
                     /locus_tag="ACHJ48_005146"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4710459.1"
                     /translation="IKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIV
                     RG"
BASE COUNT          279 a          321 c          216 g          331 t
ORIGIN      
        1 tagtctgcca gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa
       61 aggtaagtat tctgcgcaat ctccccgact gtcgccggtt cggtaacgct taattcatta
      121 aacaccactc tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt ttcccttttc
      181 cggttaacgt gacataccaa taactcttgt cgaaaaagcc agcaagttga aagaccggta
      241 ttagcaacca ccagcgcgtt taacgccccg tgccgttttt cagtcataaa aaaacccgca
      301 aaaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
      361 gcttaccgga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      421 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      481 gtctttcaca ggttcttaaa ctccattccg gatgtgcatc attcagcaat tcagccattt
      541 tgcgcttagt catcccccgc cccacataac gctgactcag aacattgagc agcccgggat
      601 aacctgccag tacttcacca ataaccctat cgattattaa cgcctctgaa tcggtacaat
      661 gtgccagcca gcttttttga ttgccgttga tcatatcccg caaaaaagcc tcaagttcag
      721 gtttgtccag acccgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      781 gctttttaga ggtcagtaat tggttgaaca tatttcccgt cttaccgtcg ccaatatacg
      841 accaacgccc ccacatacgc agtttcccct ggatccagac actttccagc gttttcaggc
      901 gtaaatgctc accgcttttg cctgtaattt ccgggtatat catatttatg ctcactcact
      961 ttcaattttg taaatcttca cgcccagccg cccaccagaa acgagctagc cgcgcacaat
     1021 attgatttca tcaaactgct cgtcgtctat gagaagtccg gcatgcgtca gcgcatccag
     1081 tggtgctttc aggatattgt ccaggtcacg acggcgctta tccggtggct ctgcaataat
     1141 ctttatc
//
LOCUS       DAZFDC010000368         1146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFDC010000368 DAZFDC010000000
VERSION     DAZFDC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.371"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            252..>1146
                     /locus_tag="ACHJ48_005147"
     CDS             252..>1146
                     /locus_tag="ACHJ48_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710460.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDSLKTDKESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEESHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKIKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPAYFPLKTNEPVTQEDVLLLYRFFKMNDDFNKLTSDE
                     YMSILTPLMKCERSVHDNNRYVTGKDTLLLDYPPSGNQIHFHVFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFT"
BASE COUNT          356 a          230 c          211 g          349 t
ORIGIN      
        1 catattttta tcaaagttaa ttgattgttg cctttatgag caccgaccgt aagcccgtta
       61 tgttattgtt tcattagtaa taatcactta aatgtatcga tgaaacaggt atgacttacc
      121 gggagtgatt gtttctgagt gaacagaggc cactttcgcc tgataaatga aaaatgagcg
      181 atttttgcaa gttagttttt ctatagccga gtacgctcag cgcctcgctt gattacaatg
      241 aggttgctgc tatgagtcct gataatatcg gttcggctgt caatcgcttc tcaggttggc
      301 catctaatga ttctctaaaa acagataaag aatcaatgac atcacccgtg actgtgcagg
      361 taataaagat gattgccgcc atccttgacc cacatcggat agtcgttgga attgatacac
      421 tttatacaac gcagcgcgaa acatttacct cagagagtca acagctcatt gaatcagcat
      481 tgcaaacact tcatgtgatg aatttaaata atctggaaac aaatcgcgat gaagaaagtc
      541 actatctggc ttttgaatat aacgttgaca cctctgacag aataaccgtt tctgcttata
      601 atgaaaaaaa acctggctgc tatggccaat gtcttgtaat acaggatgac gaaacagact
      661 tatttaacct gataaaaatt aaaattttcg aagccaggca taattatgtc gctcaattga
      721 tgagcgatcc ggaatttatg ttcaaattca gttatagcgc tcaacaaaat agattagaac
      781 ctcatcttct ggttcccgct tatttccctc taaaaacaaa cgaaccggtt acgcaagagg
      841 atgtactgtt actttatcgt ttctttaaaa tgaatgatga ttttaataaa ttaacatcag
      901 atgaatacat gagtatcctg acacctctga tgaagtgtga gcgtagcgtc cacgacaaca
      961 acaggtatgt gacgggcaaa gatacccttc ttctggacta tcctccttct ggtaaccaga
     1021 tacatttcca tgttttccct gacgaatcag caacactagt cctttatttt tcaaacaatg
     1081 acattgaatg tttcgtcttt gaaagagata ttccgagcca gtatcgcttt ttcaaaatgt
     1141 ttacca
//
LOCUS       DAZFDC010000369         1141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFDC010000369 DAZFDC010000000
VERSION     DAZFDC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.372"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1141)
                     /gene="espX6"
                     /locus_tag="ACHJ48_005148"
     CDS             complement(<1..>1141)
                     /gene="espX6"
                     /locus_tag="ACHJ48_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX6"
                     /protein_id="HFO4710461.1"
                     /translation="QFIKIMEQKAQLPIYIDKNNLKESIFSVLHNDPQQVDKDQHLPC
                     EKFLKHACKSSNSFEVKLDATHQYQHLNNFMISFDPVENQLTIRDNNNKTETFSFTNL
                     QWENLLQYYKENHQQPNIAGSRNLTDNIDKIKNTISTSEIIECASPEIRSSVLNDLYS
                     IANFLPDNNLTPNESWKRFCETCERFYVAQKSITGDKSERLTRKLSISDAGITMTFKI
                     GDVVINTISTAIPEDATGQRCIEGLNLAEMDLTDIDLSKMALRNVNFNGSILRNAKFS
                     GTICEGVDFTDCDLRNAEFENASLENNDFRKVRHLTYVNFKNANLRNSNFNGKVLTGV
                     TFTGSDLSNAYLEHIDFTTVILYETSKIPGIPGTPQIPGTPKVILT"
BASE COUNT          308 a          201 c          224 g          408 t
ORIGIN      
        1 gtaagaatta ctttaggtgt tcccggtatt tgaggtgttc caggtattcc aggtatttta
       61 gatgtttcat atagaatcac ggttgtgaaa tctatgtgtt caagatacgc gttactaagg
      121 tcacttccag taaaggttac gccagtgaga acttttccgt tgaagttact gtttcgtaga
      181 tttgcgtttt tgaaatttac ataagtcaag tggcgaactt tacgaaaatc attattttct
      241 aatgaggcat tttcgaattc tgcattacgc agatcacaat cggtaaaatc cacgccttca
      301 cagatcgtac cggagaactt ggcatttcta agaatgctgc cattaaaatt gacattcctt
      361 agcgccattt tcgacaagtc tatgtcggtt aaatccatct ctgctaaatt caacccttcg
      421 atacaccgtt gacccgttgc atcttcagga atagcagtgc taatagtatt gatgacaaca
      481 tcacctatct tgaaggtcat tgtaattcct gcatcagaga tagagagttt tcgcgtaaga
      541 cgttcactct tatctccagt gatactcttc tgagcaacgt aaaagcgctc gcatgtctca
      601 caaaatcttt tccagctctc atttggggtc agattattgt ccgggaggaa attagcaatg
      661 ctataaagat cgttcaggac gctacttctt atttcaggag aggcgcattc aataatttca
      721 gaggtggata ttgtattttt aattttatct atattatccg tgagatttcg tgatcctgct
      781 atatttggct gctggtggtt ttctttgtag tattgcagca aattttccca ttgtaagttt
      841 gtaaacgaga aagtttcagt cttgttattg ttatcccgta tggttaattg attttctact
      901 gggtcaaaag aaatcatgaa gttattcagg tgttgatatt gatgagtggc atctaatttc
      961 acttcaaatg aatttgaact tttgcaggca tgttttaaaa acttttcaca gggaaggtgt
     1021 tgatctttat ctacttgttg tgggtcattg tgcaaaacag agaaaatact ctctttcaaa
     1081 ttgttcttgt caatatagat tggtagttgc gctttttgtt ccattatttt aataaactgc
     1141 t
//
LOCUS       DAZFDC010000370         1139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFDC010000370 DAZFDC010000000
VERSION     DAZFDC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.373"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..1097
                     /gene="espW"
                     /locus_tag="ACHJ48_005149"
                     /pseudo
     CDS             39..1097
                     /gene="espW"
                     /locus_tag="ACHJ48_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
BASE COUNT          451 a          209 c          189 g          290 t
ORIGIN      
        1 aatcaccata taacaaacat atagcgagga gactatttat gcccaaaata tcatcagttg
       61 tatcatcatg ttaccatctg ttcagtgaac atcaacaact ttcaaatgaa acaacaatga
      121 cgaaccccgt ctccagaaga attgttcata aagaatatgg tatatcttta aaatccgttc
      181 ctgtatggtt ggctacagct aaaactcccc ttgctctact caatggcaga catacaagaa
      241 gtcactcatt tattattgca gggaccccag gaatgggaag caggagcgga gcccaatact
      301 atgccataaa cagtgatgat aaacgctccc gaatagacat tgactcttta ttttaaaaaa
      361 agttaaataa tgtgcgaaat caaaataaat ttccaatcga tgtaaaagaa acggttataa
      421 aactacaagg gcaaaaattc acatgcattg aagattttta taaaaggtat aatgaaacca
      481 ggttaaaggc caataccaat atccaacaag aacaaattgc agatgaagta aaatcactta
      541 catatttgat tccttcagaa aaaaaagaga tgtggatata taaaaacaat ggaaaagata
      601 atgcaaaacc aaacttagga gagcgagacg taagaatgtt cgaaaatatt agttctgatg
      661 atacagataa gataacagga aggaaatttt cagagttagg tgagtatctt tattcaggga
      721 atgtaataaa actcagtcag ttatcaattc gttacttacc caatatcagc tcaatctcat
      781 taatagagac aaaacagagt ttgttgctac atcgattgta ttcagatgaa gtacttcaga
      841 gaaatggaac gcttatcccg acaccactac atgaagaaaa atcaattcca gctgacaata
      901 taaaaacaat gctcaacaac ataccgactt acaaaatgtt accgccattc acagaaacac
      961 aaggtaattg ttcttctggc gcagccacgt ttttacgcaa atcaggcgcc gaagaaaaag
     1021 atattcttgc atgtagcccc cgaaattatg ggctgcatca taacataaaa acatgggacc
     1081 ccttggttag aaattaagga tccaaatatt atatatttca tataaagcaa gaaaaaaat
//
LOCUS       DAZFDC010000371         1137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFDC010000371 DAZFDC010000000
VERSION     DAZFDC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.374"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1137
                     /locus_tag="ACHJ48_005150"
     rRNA            <1..>1137
                     /locus_tag="ACHJ48_005150"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          284 a          268 c          361 g          224 t
ORIGIN      
        1 agctaatacc gcataacgtc gcaagaccaa agagggggac cttcgggcct cttgccatcg
       61 gatgtgccca gatgggatta gctagtaggt ggggtaacgg ctcacctagg cgacgatccc
      121 tagctggtct gagaggatga ccagccacac tggaactgag acacggtcca gactcctacg
      181 ggaggcagca gtggggaata ttgcacaatg ggcgcaagcc tgatgcagcc atgccgcgtg
      241 tatgaagaag gccttcgggt tgtaaagtac tttcagcggg gaggaaggga gtaaagttaa
      301 tacctttgct cattgacgtt acccgcagaa gaagcaccgg ctaactccgt gccagcagcc
      361 gcggtaatac ggagggtgca agcgttaatc ggaattactg ggcgtaaagc gcacgcaggc
      421 ggtttgttaa gtcagatgtg aaatccccgg gctcaacctg ggaactgcat ctgatactgg
      481 caagcttgag tctcgtagag gggggtagaa ttccaggtgt agcggtgaaa tgcgtagaga
      541 tctggaggaa taccggtggc gaaggcggcc ccctggacga agactgacgc tcaggtgcga
      601 aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgtcgac
      661 ttggaggttg tgcccttgag gcgtggcttc cggagctaac gcgttaagtc gaccgcctgg
      721 ggagtacggc cgcaaggtta aaactcaaat gaattgacgg gggcccgcac aagcggtgga
      781 gcatgtggtt taattcgatg caacgcgaag aaccttacct ggtcttgaca tccacagaac
      841 tttccagaga tggattggtg ccttcgggaa ctgtgagaca ggtgctgcat ggctgtcgtc
      901 agctcgtgtt gtgaaatgtt gggttaagtc ccgcaacgag cgcaaccctt atcctttgtt
      961 gccagcggtc cggccgggaa ctcaaaggag actgccagtg ataaactgga ggaaggtggg
     1021 gatgacgtca agtcatcatg gcccttacga ccagggctac acacgtgcta caatggcgca
     1081 tacaaagaga agcgacctcg cgagagcaag cggacctcat aaagtgcgtc gtagtcc
//
LOCUS       DAZFDC010000372         1108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFDC010000372 DAZFDC010000000
VERSION     DAZFDC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.375"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(180..710)
                     /locus_tag="ACHJ48_005151"
     CDS             complement(180..710)
                     /locus_tag="ACHJ48_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO4710462.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(719..1078)
                     /locus_tag="ACHJ48_005152"
     CDS             complement(719..1078)
                     /locus_tag="ACHJ48_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO4710463.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
BASE COUNT          279 a          315 c          206 g          308 t
ORIGIN      
        1 ctgaaagaac ggtattaata accaccagcg aatttattgc gctgctgtat attacggaca
       61 caaaaaaacc accttccggt ggcttccttg tgcgaaaaaa cttgcatttc gcctcgcgat
      121 acagctttgc gaagcttaca ggaattcaag ctgtttctgc gtaaaaaagc aagctttttt
      181 tatcgaaatg aatcgtgcat aggtacataa agcatgtgtt cagccacggc taaccaacct
      241 gcaatacgtt tctcacatgt gctgaaacac cattccgggt gagtacgatt taaacattct
      301 gccatttttc tcttactcat tccccgtcct tcgtaccttt gccggagaat attgattagc
      361 ccgggatatt ccccaagcac ctcactgata acgcgatcaa taatcaacgc ctctgtgtct
      421 gtacaatgtg acaaccagct cttctgcttc cctctggtca tatcccgaaa aaatgcctca
      481 agttccggtt tttccagccc ggatttcttc atgctgcgta aaacctcatt aactgctgtt
      541 ttcgtcagct ttttcgaaac cagtaaccgg ttaaacatat ttccggattt acccccaccg
      601 atatacgacc accgccccca catccgtaat ttcccctgga tccagactgc ttccagcgtg
      661 ttcaggcgta aatgttcgcc gcttttgcct gtaatttccg gatatatcat atttacgctc
      721 actcactctc aattttgtaa atcttcacac ccagccgtcc accagatact ggctgaccac
      781 gtacaatatt gatttcatca aactgctcat cgtccattaa cactcccgca tgcgtcagcg
      841 catccagcgg tgctttcaga atattgtcca ggtcacgacg acgcttatcc ggtggctctg
      901 caataatttt tattgccagc cgtccggaca ggcttaattt cagtcgctgc tggcgaacaa
      961 taagcaccac tgcccggcga taacgctccc cggcttttga tacaaaatat gtgctgccac
     1021 ggcgtcgcca gtaagtgttc accgtcggcg ggtaaggtaa aaccaaatct atgagcatca
     1081 gtcacctctt ttacccgagc acgccagt
//
LOCUS       DAZFDC010000373         1104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFDC010000373 DAZFDC010000000
VERSION     DAZFDC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.376"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..655)
                     /locus_tag="ACHJ48_005153"
     CDS             complement(<1..655)
                     /locus_tag="ACHJ48_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4710464.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDKPTTDIRKDRCSKCMRGCEMRGMV
                     "
     gene            complement(655..984)
                     /locus_tag="ACHJ48_005154"
     CDS             complement(655..984)
                     /locus_tag="ACHJ48_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4710465.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
     gene            complement(981..1104)
                     /locus_tag="ACHJ48_005155"
                     /pseudo
     CDS             complement(981..1104)
                     /locus_tag="ACHJ48_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305458.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          223 a          404 c          243 g          234 t
ORIGIN      
        1 ccaccatgcc gcgcatctca cacccgcgca tgcatttact gcatctgtcc ttccggatat
       61 ccgtggtggg tttatcgaac tcatccgcca ccgcaggacc gttatacccg cattcatctc
      121 cccggtaatc ccacatacag gtgttcgcca gcatgatgcg accgggaaac agcgctccgt
      181 ccgtctccgt cggtgtcgcc agcacaaacg aggccgtcat ggccgtcagc gatgacatct
      241 gctccaccac ccaccggtcc gtcagctcct gctccgggtc tgcctcagga ttgcctgcca
      301 caaaattcac cgcatccaga aaacgcgcat acacccggcg gcggaccacc gtggcaccca
      361 ccaggctctg caaatcctcc gccatcccgg tgacaaggcc gaaaagattg gacactgtca
      421 gcgacggtct ggcactgctg cccttcccgt tcatctcaaa accgctgccg tcaatcgggt
      481 atacctgata ttgccgcccc tgccaggtga ccgcctcccc tttttcattc agctcattac
      541 agaaaaaata ccgctcaccg ccctgcaccg tcaggtcaat ttcccagagc accacccgcg
      601 gtgactgctc tgatttaacc gactcgttca ggctttcttc gtgaatatcc tgcatcagtt
      661 caccacctgc ttaaactcgg cactgaattc aacccgcagc atgcgaaccc gtgatgacca
      721 ggcggcacag gtcaccttta tctgccggta tgcatagggt ggcttccaca aaaatgcctt
      781 ccagcctccg tgctctgcca ggaacgcttc cagatgccgg gcctcctccc gggtcacgga
      841 aagcgtcaca cggtatgttt tcaggtcagc attcagccct gccgccatac gctgtgagta
      901 cccgtcacca aaacgcactt cacgcaccga cggctgcgag ttcacctcca tatccggctt
      961 cactttccag cgaaatgttt tcatcgcccg ctccccgata acataccgca tcacgcaact
     1021 gcgcctgaat ctcatcctgc gcccccttgc gggccatgtc atacaccgcc ttcatcagct
     1081 gcggccccgc ctgtccgttg gtgc
//
LOCUS       DAZFDC010000374         1101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFDC010000374 DAZFDC010000000
VERSION     DAZFDC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.377"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..486
                     /locus_tag="ACHJ48_005156"
     CDS             <1..486
                     /locus_tag="ACHJ48_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO4710466.1"
                     /translation="INEDGSPYLLRTHQLRHLLNTFAQINGMDEFSIARWSGRKLISQ
                     NVSYDHRSHLQMSKAIREKKLSVCVNEHRIKDIPVVDLNEFDSLSSGAVLVSKHGYCK
                     HSYAFKPCDNYPIKNSGLDNETISNIHDKILKRTLYDKNDGNINADKWYEFHKKIKKG
                     E"
     gene            487..885
                     /locus_tag="ACHJ48_005157"
     CDS             487..885
                     /locus_tag="ACHJ48_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710467.1"
                     /translation="MAKHINGYHEKKIVDVINTWSIDEKLTWNALCDRLVRVIGKRPS
                     RQSLSSHVRIAESFNVKKTTIKSGVIHTVKPANLKVASQRIKRLEAENEALKALNERY
                     LEQFKVWLYNAHLKQITIEELNNPLPAKYL"
     gene            983..>1101
                     /locus_tag="ACHJ48_005158"
     CDS             983..>1101
                     /locus_tag="ACHJ48_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710468.1"
                     /translation="MDKTNINLMERYLLLLDRFVDKLAESGFSEQRIIEQSYL"
BASE COUNT          412 a          163 c          199 g          327 t
ORIGIN      
        1 ataaatgaag atggtagccc atatttgcta agaacacatc aactaaggca tcttctcaac
       61 acatttgctc aaataaatgg tatggatgaa tttagtattg ctcgctggtc tggacgtaag
      121 cttatttctc aaaatgtttc ttatgaccac agatcgcatc ttcaaatgtc taaagcaata
      181 agagaaaaaa agttatcagt atgtgttaat gagcacagaa taaaggatat tccagtagtg
      241 gatcttaatg agtttgactc acttagtagt ggtgcagtac ttgtatcaaa acatggctac
      301 tgcaagcact catatgcgtt taagccgtgt gataattatc caattaagaa ctctggttta
      361 gataacgaaa cgatttcaaa tatccacgat aaaattttaa aaagaacact gtatgataaa
      421 aatgatggga acataaatgc tgataaatgg tatgaattcc ataaaaaaat aaaaaaagga
      481 gaataaatgg ctaagcatat aaatggttat catgagaaga agattgtaga tgttattaac
      541 acatggtcta tagatgaaaa actaacctgg aatgctttgt gcgataggtt agtcagggtt
      601 attgggaaaa gaccatcaag acaatcctta agtagtcatg taaggattgc ggaaagcttc
      661 aatgtgaaaa aaaccacaat caaatctggt gtaatacaca ctgtaaaacc tgctaattta
      721 aaagtggctt ctcaacgaat taaaagactt gaagcagaaa atgaagcttt gaaggcatta
      781 aatgaacgct atttagagca gttcaaagta tggctttata atgctcacct taaacaaata
      841 acgattgaag aacttaataa tccgttacct gcaaaatacc tttaataaaa ttgataggtt
      901 ttagctattg acatggtgaa atattatgtt atttattttc ttgtcaggac gacaaaattt
      961 cactattgca aggatgcata ttatggacaa aacaaatatt aacttaatgg aaaggtatct
     1021 tcttctgctt gatcgattcg ttgataaact cgctgaatca ggtttctcag agcaacgaat
     1081 tatcgaacaa tcatatcttt t
//
LOCUS       DAZFDC010000375         1100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFDC010000375 DAZFDC010000000
VERSION     DAZFDC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.378"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(202..1056)
                     /gene="rclR"
                     /locus_tag="ACHJ48_005159"
     CDS             complement(202..1056)
                     /gene="rclR"
                     /locus_tag="ACHJ48_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO4710469.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWSVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          309 a          275 c          243 g          273 t
ORIGIN      
        1 cgacaataat cacgtatcaa gtaatagtcg ggattttatt taattgaagc aatttagttt
       61 gcatttttat ttaaaaactg agctagagtc catcagaaaa aaatatataa agcccaaacc
      121 accgttggaa atagaaatag ttgaaaggct cacaactccc gaaatgcgga ttatctttgc
      181 cctgatttct ggcggtttta ttcagggtgc aagctgtctg accctttccc gatattctcc
      241 cggagtacaa ccaaactcgc ggacaaacgc cttgtgaaaa gatgattcac tggcatagcc
      301 tactgactca gcgatcacca caacagggag catttcccgg gaaaacatct gggccgctat
      361 ttgtagacgc aactttgtta atacagccag cggcgtggtt ccggaaacat cacggaaaag
      421 ctgggcaaaa cttgcccggg acatgtgggc gatgctggcc agcgattcga cgctccaggc
      481 gtgtcctggc atttccagca tttgctgtat taccgcacca agacgtggat gcagaagcaa
      541 actgagaatg tttttctcag tattaacctg tgcaatccac tcacgcaccg cgagggtaaa
      601 gaatgtagcg cagatctggc tacacagtgc atccactccc ggcattgccg atctggattc
      661 ctgttgtaaa aacgggatcg cctccctcag ccagttatat tccacgctgt ggttaaccgg
      721 tgctaaaaac agcgtttccg gcaaagacgt taaaaaataa cgtgccgaat gttgcagccg
      781 aagagtacca cagacaatac aggtcgattc attatcgaca tgactcaggc gatgagctga
      841 attttgtggt agcaggacca catttcccgg gcgtaatgta aaaatctctc ccgtcggcat
      901 ttccagcttc gccgctcctt gcgttaacgc atgccaacga ataactgata attccccggc
      961 accatgtgga agctgccagt cacttcctaa cacgcaattc ttatcgatcg ttccttgtgg
     1021 agcgttaagc atcaacaaac ggctaagggc atccatatca gactcctgag tggtttttga
     1081 ttacaaacac caacaatctt
//
LOCUS       DAZFDC010000376         1098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFDC010000376 DAZFDC010000000
VERSION     DAZFDC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.379"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..116
                     /locus_tag="ACHJ48_005160"
     CDS             <1..116
                     /locus_tag="ACHJ48_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HFO4710470.1"
                     /translation="DFFIKVDTSINFYVTRQNDYNYVFSTGRTPRFICISH"
     gene            complement(218..>1098)
                     /locus_tag="ACHJ48_005161"
     CDS             complement(218..>1098)
                     /locus_tag="ACHJ48_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO4710471.1"
                     /translation="TVTTPMISLYPYVISNVFYILPLSILLSFILYFLWQRWISRKMS
                     LAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKNISPVVFIRAAEEE
                     NLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVADVLHIKNAISDKF
                     NIVLEITERNLVEDTDHALQKINELRSHGCEFAVDDFGTGYCSLGLLQKLSVDYLKID
                     KSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAEGVSTSHQAEYLIHNNVTLVQGYLY
                     AKPMKATEFYQWYVNR"
BASE COUNT          344 a          218 c          182 g          354 t
ORIGIN      
        1 ctgatttttt cataaaagtt gacacctcaa ttaattttta tgttacacgt caaaatgatt
       61 acaactacgt attctctaca ggcaggacac cacgcttcat atgtatttcc cactaacagc
      121 attgttgctc ctggcgtttg tcaccagtta ataaccttct gatggctttg tttatatttt
      181 aaggcaataa aaaaccattc caatattggt taattattta tctgtttaca taccattgat
      241 aaaactctgt cgctttcatt ggttttgcat ataagtatcc ctgaactaat gtcacgttat
      301 tatgaataag atattccgcc tggtgagagg tactgacacc ttctgcaatc gttatcaaat
      361 tgagtcgatt actcagctta ataataatat ctaaaacagg cgtttcgtct tccgctgtag
      421 tgagcgtgtc tataaagctt ttatctattt taagataatc aacagacaac ttttgcagaa
      481 ggccaaggga acaatatccc gtgccaaaat catcaactgc aaattcacag ccatggctcc
      541 ttaattcatt tattttctgt aatgcatgat cagtatcttc aaccagattg cgttcggtaa
      601 tctcaaggac aatgttaaat ttatcagata ttgcattttt tatatgtaaa acatcagcaa
      661 caaaatcagg atgtgcaaga tgttcagcgg caatatttat cccaaggtga aacggctttt
      721 tcactttcca gctttgtaca tcctgaataa tcagttcaaa gagatgctta gttaacggta
      781 taattaaatt ttcctcttct gcagcgcgga taaaaactac cggagatata tttttaccat
      841 cttcgcgttg ccaacgcatt aacgcctcaa cccccagaca cgctttggtt gtcgtgtcac
      901 atactggttg ataatgcaca gaaaattctc cggatgacat tcctttctta atttcttctg
      961 ccagggacat tttccgagat atccagcgct gccataagaa gtacaggata aaagaaagaa
     1021 gaatagataa tggaagaatg taaaatacat tactaatgac atagggatat aatgaaatca
     1081 tcggcgtagt aactgtaa
//
LOCUS       DAZFDC010000377         1090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFDC010000377 DAZFDC010000000
VERSION     DAZFDC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.380"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            42..572
                     /gene="yodB"
                     /locus_tag="ACHJ48_005162"
     CDS             42..572
                     /gene="yodB"
                     /locus_tag="ACHJ48_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO4710472.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIVAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          326 a          238 c          209 g          317 t
ORIGIN      
        1 cgtgggatag cttgactgtg aaaattacag gagctacaaa aatgaaccga ttctcaaaaa
       61 ctcaaattta tttacattgg ataacgctgc ttttcgttgc aataacctat gccgcgatgg
      121 aactccgtgg ctggtttcct aaaggtagta gtacttatct gctgatgcga gaaacacatt
      181 acaatgcggg tatattcgtt tgggtgttaa tgttttcacg cctgattata aaacaccgtt
      241 atagtgatcc ttctattgtg ccaccgccac ctgcctggca aatgaaagcg gcttcgctaa
      301 tgcacatcat gctttatata accttccttg cgcttcctct gctggggatt gctttgatgg
      361 cttacagtgg aaaatcgtgg agtttccttg gtttcaatgt gtctcccttt gttaccccaa
      421 acagcgaaat taaagcactg ataaaaaata ttcacgaaac ctgggcaaat ataggctact
      481 ttttaatcgt agctcacgct ggcgcagcac tctttcatca ctacattcag aaagataata
      541 ctctgttacg aatgatgcct cgccgcaaat aagtcgatcc tctgacaaag gaaatgtgaa
      601 ccatggacat caacgcatca taaatgttta cgcaagtgca taccgcactc tttccactat
      661 caacaaggag gagcagaaaa tgtgtaaatc gtacaatggt aagacttatc aaaaatacag
      721 gttaattttg gtatttaaac cttttttact tgttagtatg atacaaaggc tttcaaaaaa
      781 gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt taatctgacg
      841 gccgcgttcc ttttttgact caattcttga ccccttctct acgcaacttc agttcccacc
      901 accaactttg cggcagtttc ataggatgaa tgtctaaaag aataatggtg agcgataaga
      961 aaacgactga ataactgcac attttcgctc gaaagcttcc tgtcagatcc atagcgaatc
     1021 aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct tcaagaatat
     1081 tctacgattg
//
LOCUS       DAZFDC010000378         1081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFDC010000378 DAZFDC010000000
VERSION     DAZFDC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.381"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(40..195)
                     /locus_tag="ACHJ48_005163"
                     /pseudo
     CDS             complement(40..195)
                     /locus_tag="ACHJ48_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            345..1013
                     /locus_tag="ACHJ48_005164"
     CDS             345..1013
                     /locus_tag="ACHJ48_005164"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO4710473.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          312 a          244 c          211 g          314 t
ORIGIN      
        1 caccactttg tctaattcgt cagattctga ccagacggtt caggctgtac gcttacgagc
       61 tactgatgta tcaacacgat tcaacaatac ccgatattta ttccatgcct ccagcaacga
      121 tctttcttcc tccgttgcga tttccagatc tacagcatcc tgcagtggcg caatatactc
      181 actgaattcc tggatgtaga actgtgtggt gacggtcttc cagccattcg gctcctgctg
      241 tatcgcagca taccaggcta tttcaatatc gctatgctgc ggcagcattt aaccccttgt
      301 aattcatcgc cataattgat ttaattcaca aataaaacta taacatggtg aaattaatga
      361 aaaaaaacac agatgatggg gctaaaattt acacaccact taccctaaag ctttatgact
      421 ggtgggtttt gggagtatca aatcgtcttg catggggatg tcctacaaag gaacaccttc
      481 ttccacactt tctggaacat ttaggtaaca accatctgga tattggtgtt ggaactgggt
      541 tttaccttac tcacgtacct gagagtagtc tgatatcttt aatggatttg aacgaagcta
      601 gcctgaacgc ggcatctaca agggctgggg aatcaaaaat taaacataaa attagccatg
      661 atgtttttga accttatccc gcggcgttac atggtcaatt tgattccatt tccatgtttt
      721 accttcttca ctgcctgcct ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg
      781 cgcaggcctt aactgacgat ggaactctat acggagccac aattcttggc gatggagttg
      841 tgcacaatag cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc atcttttcaa
      901 acacaaaaga ttccgaagaa ggcttaacac atatactctc agagcatttc gagaatgtta
      961 aaaccaaggt tcaaggtact gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa
     1021 ccgcagcacg ttcttgcata cgccgtgtct gcggtttaat ttttactctc aaatattgta
     1081 g
//
LOCUS       DAZFDC010000379         1075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFDC010000379 DAZFDC010000000
VERSION     DAZFDC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.382"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1075
                     /locus_tag="ACHJ48_005165"
     CDS             <1..>1075
                     /locus_tag="ACHJ48_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710474.1"
                     /translation="FPCTQLNQSDNFLDSLREFFAILNSSRKGELSTWDTIYLHLILV
                     INADSDLIKNDVLLAENIPTADYQFNTFFSNAFETDVKKYLGKSEDNEVEIKTGNERI
                     SIGIRNRSNGKLERQKFLFPLDYENKLQHQLDKHFTIESHPLLYRHTMGSKIANVIFE
                     KLYSRIDFNKEQYISFIKDAFLHFYDYSRRYAINENIAKDAVTNNIALMSTFYDSDNT
                     SGEVLNNDFTEESFETALDVEHTIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLF
                     PEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLINLKT
                     LLNDGCFIKYSHFNDVALIKQNI"
BASE COUNT          374 a          160 c          199 g          342 t
ORIGIN      
        1 catttccatg cacacaattg aatcaaagtg ataatttcct cgattcactg agggaatttt
       61 tcgcgatatt aaattccagt cgtaagggtg agttgtctac atgggatacc atctacttgc
      121 atctgatatt agtaataaat gctgacagtg atctcataaa aaatgatgtg ttgctcgcgg
      181 aaaatattcc aacagcggat tatcaattta atacattttt tagtaatgct ttcgagactg
      241 atgttaaaaa atatctgggt aaatcagaag ataacgaagt tgaaataaaa acgggtaatg
      301 aaagaatctc tattggaata cgtaacagat cgaatggaaa acttgaaaga caaaagtttc
      361 ttttcccact ggactatgag aataaattac aacatcaatt agacaagcat tttacgattg
      421 aatcgcaccc attattatac aggcatacta tgggtagtaa gattgcgaat gtgatttttg
      481 aaaaattata cagtcgtatt gactttaaca aagaacaata tatatccttt ataaaggacg
      541 cgtttcttca tttttatgat tatagcaggc gatatgcaat taatgaaaat attgcaaaag
      601 acgctgtaac aaacaatatc gcattgatga gcacttttta tgacagtgat aatacgtcag
      661 gtgaggtttt aaataatgat tttacagaag agtcatttga aactgcgtta gatgtagagc
      721 atacaatagt gcttggattt gctgatgata attttgaaac aaaacctgtt cattatcagg
      781 atttattaac acgatttagt gcctttcaag ataccgtgtt taatctgttt cctgaaatgc
      841 actcttcgca ttacaacgat atttgtagcg taagtgtgga tatgacaaag ggaacgcaat
      901 gcatgattca cttgatggtg aatgaagaag ttttcatgtc tctgcctgtt ccagttgcca
      961 cgatggtacg tgaagatgca agtaatttaa tcaatcttaa aacactactc aatgatggtt
     1021 gctttattaa atatagtcat tttaatgacg tggcattaat aaagcaaaac ataag
//
LOCUS       DAZFDC010000380         1074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFDC010000380 DAZFDC010000000
VERSION     DAZFDC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.383"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(261..908)
                     /gene="ychE"
                     /locus_tag="ACHJ48_005166"
     CDS             complement(261..908)
                     /gene="ychE"
                     /locus_tag="ACHJ48_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO4710475.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(939..992)
                     /gene="ychE"
                     /locus_tag="ACHJ48_005167"
     CDS             complement(939..992)
                     /gene="ychE"
                     /locus_tag="ACHJ48_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO4710476.1"
                     /translation="MLSKGVLSARLFNLIYG"
BASE COUNT          345 a          250 c          217 g          262 t
ORIGIN      
        1 ttattattta gcactttcct ctaccaaagg cagtggaatt actaatatca tttcaattat
       61 tcttcagcaa gcccaggatt atgatgtggc gaaaataaca tgatgatgag gagaagcagt
      121 taaggaaatc cttataacta actgtaattt gagaagaata tttttaccta ttccttttgc
      181 ttttgataac tgcgcgggaa caaagtaaaa ataatgacga agtaaacgga gcatggcagc
      241 tccgtttcat ttgaaaggaa ttaattaagc aggccgggga aaatcccctt aataccagta
      301 acgataaatt caatccccaa tgccatcagc aatagcccca taatacgcgt aatcacgttg
      361 atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca tgcggaacaa tccccaacaa
      421 cataaagcga acaatgcaat agccacaaag aaaccaaaca gatagctaat gctgtgataa
      481 cgcgtacccc agacgatggt agaactgatc gcccctggcc ccgccatcaa cggcaacgcc
      541 aatggcacca caccaatact ttcacgtacc gcggtttctg atttttcttg cttgttctgt
      601 ttatcctcgc caagcttgcc gctgatcatc gacatcgcta tcgtcaccac caggatgccc
      661 ccggcgatac ggaacgaatc aattgatata ccaaaaagtt gcagaatcgt gtcgccgaga
      721 aaaagcgaga tccacaagat aatggccaca gacaggttgg ctgtaaggtt agttttgttt
      781 cgcgctgccg ctgtctgata actggtcatg ctgataaaga cgggaataat ccctaccggg
      841 ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa cgggaaaatc aaaaagggtc
      901 tgaatcacgg ttagctccga agcaaaagcc ggataatgtt agccataaat aaggttgaaa
      961 agacgcgctg acaatacgcc ttttgacagc atttttcacc tcctaactac ttaaaattgc
     1021 tatcattcgt tattgttatc tagttgtgca aaacatgcta atgtagccac caaa
//
LOCUS       DAZFDC010000381         1068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFDC010000381 DAZFDC010000000
VERSION     DAZFDC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.384"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..398
                     /gene="yqeJ"
                     /locus_tag="ACHJ48_005168"
     CDS             <1..398
                     /gene="yqeJ"
                     /locus_tag="ACHJ48_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO4710477.1"
                     /translation="KMIYNETCTANWVIFNDQGRANLTIDFMYNKKNKTGTVALSGTW
                     QQGNRESKSIRRNIEYTWVENYDTAHLTSKKVNKFEIMDQVDDDRLAELIPDFYVFPE
                     KSVSYNILKQGKHAFILSIGNRAIMHCAR"
     gene            complement(431..511)
                     /locus_tag="ACHJ48_005169"
     CDS             complement(431..511)
                     /locus_tag="ACHJ48_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710478.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(547..972)
                     /gene="yqeK"
                     /locus_tag="ACHJ48_005170"
     CDS             complement(547..972)
                     /gene="yqeK"
                     /locus_tag="ACHJ48_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO4710479.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          393 a          181 c          166 g          328 t
ORIGIN      
        1 aaaaaatgat atataacgaa acatgcactg caaattgggt aatttttaat gaccagggac
       61 gagcaaatct taccatagac tttatgtata acaaaaaaaa taaaacagga actgttgcgt
      121 tgagtggaac ctggcaacaa ggtaacagag aaagtaaatc aatacggcga aatattgaat
      181 acacatgggt tgaaaactat gacacagccc atttaacatc aaagaaagtt aataagtttg
      241 agattatgga tcaagtcgat gatgatagac ttgcagaact tattcctgat ttttatgtct
      301 ttccagaaaa aagtgtaagc tataatatac taaagcaagg taagcatgct tttattttga
      361 gcattggtaa cagagcaata atgcattgtg caaggtaaac taaaatagat aacaaaaaga
      421 tggaacctcg ttaatcataa aacaataaaa ttaaatattc tcgcagtata tggcagtcta
      481 aagcatcaaa gttttgatca acatctttca ttttagacat ctccttacaa tgtaaaatat
      541 catgtatcaa caacatctgg tgcaaatcca tgagtcgaac tcgacattca tcttagctcg
      601 attattattc cttcgtacag tcgatgtaaa caatacagaa agaggattat taagaaccag
      661 tttactcagt gaatcaatag aggaaaggac taacgtttct ttaaaagaat tgatttcatc
      721 atctgttaaa ctaaactcat cattgacaga tcgtgagata taactgtttt taactttact
      781 ctttacgttg actttattga cagaattaac attcacaaat cttgaattta atgcccatga
      841 tgttgtttgc gtgagaacct tctcagcatt aaggaatttt tttaacggaa tccttggttt
      901 ctttttcgag tccgaattta caatatcatg catatagacc atttcatgaa tctgcgaaaa
      961 ctcaatgtcc attatatacc tcacattttt accgtgactc gatgttactg ttcaataatc
     1021 accttccatc aatactaaaa ttaatacccc taatgtgccg ataacaaa
//
LOCUS       DAZFDC010000382         1066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFDC010000382 DAZFDC010000000
VERSION     DAZFDC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.385"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..100
                     /locus_tag="ACHJ48_005171"
     CDS             <1..100
                     /locus_tag="ACHJ48_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4710480.1"
                     /translation="RIPTASQNRSPAPVFVVMSGEYLLDKKEEIIQ"
     gene            113..202
                     /locus_tag="ACHJ48_005172"
     CDS             113..202
                     /locus_tag="ACHJ48_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710481.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            222..>1066
                     /gene="nleL"
                     /locus_tag="ACHJ48_005173"
     CDS             222..>1066
                     /gene="nleL"
                     /locus_tag="ACHJ48_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO4710482.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNL"
BASE COUNT          317 a          174 c          223 g          352 t
ORIGIN      
        1 acgtattccg acagcttcac aaaaccggag tccggctccg gtttttgttg tcatgtccgg
       61 ggaatatttg ttagataaaa aagaggagat aattcaatag ggagttaaat taatgccgat
      121 aaatctgaca tcttatttgt gggtacaggg acagaaagtt gtcccggcag ttgtgttttc
      181 taattttact ttagtggact aagtaaaaag gagtgagata aatgctgccc actacaaata
      241 tctctgtaaa ttctggagta atatcttttg aaagtcctgt agattcacca tctaacgagg
      301 atgttgaagt tgccctcgaa aagtggtgtg ctgagggaga atttagcgaa aatcgtcatg
      361 aggttgcatc aaaaatactt gatgttataa gtactaatgg agagacttta tcaatcagtg
      421 agccaataac aacattacca gacttgcttc caggttctct gaaagaactg gttttgaatg
      481 gatgtacaga gcttaaatca ataaactgct taccccccaa cttatcttca ttaagtatgg
      541 ttggatgctc atcattagag gttataaatt gcagcatacc tgaaaatgtc attaatttat
      601 ctttatgcca ttgtagttct ttgaaacata tagaaggttc ctttcctgag gcactcagaa
      661 attccgtata tttaaatggc tgtaattcat taaatgaatc gcaatgtcaa ttccttgcat
      721 atgatgtcag tcaaggccgt gcctgcctga gcaaagctga gcttactgct gacttaattt
      781 ggttgtcagc taaccgaacg ggtgaagagt ctgctgaaga attgaattac tctggatgtg
      841 acttgtcagg tctaagtctt gtagggctga atttatcatc agtaaatttt tctggagcag
      901 tgcttgatga tacagatctc aggatgagtg atttgtctca ggctgtattg gaaaactgtt
      961 cttttaaaaa ctcgattttg aatgaatgta atttttgtta tgctaattta tctaattgta
     1021 ttattagggc tttgtttgaa aactctaatt tcagcaattc caatct
//
LOCUS       DAZFDC010000383         1064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFDC010000383 DAZFDC010000000
VERSION     DAZFDC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.386"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..308
                     /locus_tag="ACHJ48_005174"
     CDS             <1..308
                     /locus_tag="ACHJ48_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710483.1"
                     /translation="GMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLP
                     VMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDAGG"
     gene            311..637
                     /locus_tag="ACHJ48_005175"
     CDS             311..637
                     /locus_tag="ACHJ48_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4710484.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            647..997
                     /locus_tag="ACHJ48_005176"
     CDS             647..997
                     /locus_tag="ACHJ48_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO4710485.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            994..>1064
                     /locus_tag="ACHJ48_005177"
     CDS             994..>1064
                     /locus_tag="ACHJ48_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710486.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGA"
BASE COUNT          224 a          245 c          377 g          218 t
ORIGIN      
        1 acgggatgcg gggatgggaa tgcctggttg acggggtggc ggatattgac gtcagcatga
       61 cgggtgagcg gttgttctct gtggtggtcc ggcagagcag tggccagcgt acggaaaaaa
      121 cattttccct gccggtgatg ctctaccgcg gtgtgttcag agccggtgaa acctaccacc
      181 ccggcgatac ggtgacgtgg gggggctcgc tgtggcactg caacagtatg accgaagata
      241 aacccggaga agctcattca tcagcctgga ccctggctgc aaaacgtggg cgggatgcag
      301 gaggctgaaa atgacggcat tactgacact ggaagagatc aaggcacatc tgcgtgtcga
      361 ccatgacgcg gatgatgaca tgctgatgga caaggttcgt caggctaccg ccgtgctgct
      421 ggcctacatt cagggcagcc gggataaggt gattcgtgag gacggtgaac tgatcccggg
      481 cgaggcatta acccggatga agggggctgc catgcgactg accgggatgc tgtaccggaa
      541 tccggatctt gcggagcggg aagaactgat tcagggggag ctgccgtttt ctgtttccgt
      601 gctgatttac gatttgcgtt gtccgacggt gttatgagga gggggaatgg caatatctgc
      661 aggtcgtctg acacagatga taagtgttct gaacccggtg ttaacccgta acgctgccgg
      721 agaaatgacg gaagaatggg tgtcatgcgg gaaaattcat gcggatatcc ggggcaggag
      781 cagccgggag cggatgcagt ccggtgcgga aatggcgcag gcggaaatcc gcatctgggt
      841 gcgcggtcag tccggtcggg aaatcacggc agcgtcacga cttcatgtgc tgagtggtcc
      901 ctggcgtgac cggatcctga acgttgtcgg gctgcccgtg ccggatgcga ccggcggacg
      961 tctggaaatt ctctgtcggc tgggagggga aaaatgatcg aaaccctgct ggatttttcg
     1021 gggctggagg acatcagccg cgatttgcag cttctgagtg gtgc
//
LOCUS       DAZFDC010000384         1055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFDC010000384 DAZFDC010000000
VERSION     DAZFDC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.387"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1055
                     /locus_tag="ACHJ48_005178"
     CDS             <1..>1055
                     /locus_tag="ACHJ48_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4710487.1"
                     /translation="LSIDAASRTLTLDREVTLPETGAATVNLINGSGKPVSVDITAHP
                     APDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEA
                     IVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGVRFSLRL
                     TSGSGEGSRLVTTAITADTEHRSSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPA
                     VPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWS
                     VSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGL
                     WELIDNSQLADEMAEMK"
BASE COUNT          224 a          293 c          342 g          196 t
ORIGIN      
        1 tcctgtccat cgatgccgcc agccgcaccc tgacgctgga ccgtgaggtg acactgccgg
       61 agaccggtgc cgccacggtg aacctgatta acggcagcgg taagccggtg agtgtggaca
      121 tcaccgcaca ccccgcgccg gaccggatac aggtcagcac cctgcctgat ggtgtggaga
      181 catacggtgt atggggacta tccctgccgt cactgcgccg tcgcctgttc cgctgtgtct
      241 ccgtccggga aaacacggac ggcacctttg ccatcacggc ggtgcagcac gtaccggaaa
      301 aagaagccat cgtggataac ggtgcccgct ttgagccgca gtcaggcacc ctgaacagcg
      361 tcatccctcc ggcagtgcag cacctgacgg tggaggtgag cgcagctgac ggccagtatc
      421 tggcgcaggt gaaatgggac acgccgcggg tggtgaaggg cgtgcgcttc agtctgcgcc
      481 tgaccagcgg aagcggagaa ggcagccgtc tggtgaccac cgccatcact gcggatacag
      541 agcatcgttc cagtggtctg ccgccggggg aatacaccct gacggtcagg gcgattaaca
      601 gttatggcca gcagggggaa ccggccacca ccacgttcag gattaatgca cctgcggtac
      661 ccgccacgat tgagctgaca ccgggctatt ttcagataac agcggtcccg cgtcttgcgg
      721 tgtatgaccc gacggtacag tttgagttct ggttttcgga gacaaaaatc gcagatacat
      781 ctcaggtgga aacctctgcc cgttatctgg ggaccggcag tcagtggagt gtatccggcc
      841 cgcacattaa gcccgggaag gatttctggt tttacgtgcg cagcgtcaac ctggtgggga
      901 aatctgcttt tgtggaagcc agtggccggg ccagcaatga tgcagaaggg tatctggggc
      961 tgtttcggga aaaaatagga aaactgcatc tggctcaggg gctgtgggag ctgatagaca
     1021 acagccagct tgcggatgag atggcggaga tgaag
//
LOCUS       DAZFDC010000385         1037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFDC010000385 DAZFDC010000000
VERSION     DAZFDC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.388"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..548
                     /gene="nleB"
                     /locus_tag="ACHJ48_005179"
     CDS             <1..548
                     /gene="nleB"
                     /locus_tag="ACHJ48_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO4710488.1"
                     /translation="ERDNYLLGLLREELKNIPEGKDSLIESYAEKREHTWFDFFRNLA
                     MLKAGSLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIK
                     SLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCD
                     FIEFKHENIIPNTSMYTCSSW"
     gene            597..>1037
                     /gene="nleE"
                     /locus_tag="ACHJ48_005180"
     CDS             597..>1037
                     /gene="nleE"
                     /locus_tag="ACHJ48_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO4710489.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIV"
BASE COUNT          337 a          156 c          218 g          326 t
ORIGIN      
        1 gtgagaggga taattatctg ttaggcttat taagagaaga gttaaaaaat atcccagaag
       61 gtaaggactc tttgattgag tcatatgcag aaaaaagaga acatacttgg tttgattttt
      121 tcaggaattt ggccatgttg aaggctggaa gtttgtttac agagacggga aaaactggat
      181 gccataacat atcgccctgt agcggatgta tatatcttga tgccgacatg attattaccg
      241 ataaattagg agtcctgtat gctcctgatg gtatcgctgt gcatgtagat tgtaatgatg
      301 agataaaaag tcttgaaaat ggtgcgatag ttgtcaatcg tagtaatcat ccagcattac
      361 ttgcaggcct cgatattatg aagagtaaag ttgacgctca tccatattat gatggtctag
      421 gaaagggtat caagcggcat tttaactatt catcgttaca cgattataat gctttttgtg
      481 attttattga atttaagcat gaaaatatta taccgaatac cagtatgtat acctgcagtt
      541 catggtaagt cttccggttg tggttgaaaa atatcgttct ttaggtatag attcagatga
      601 ttaatcctgt tactaatact cagggcgtgt cccctataaa tactaaatat gctgaacatg
      661 tggtgaaaaa tatttacccg gaaattaaac atgattactt taatgaatca cccaatatat
      721 atgataagaa gtatatatcc ggtataacca gaggagtagc tgaactaaaa caggaagaat
      781 ttgttaacga gaaagccaga cggttttctt atatgaagac tatgtattct gtatgtccag
      841 aagcgtttga acctatttcc agaaatgaag ccagtacacc ggaaggaagc tggctaacag
      901 ttatatccgg aaaacgccca atggggcagt tttctgtaga tagtttatac aatcctgatt
      961 tacatgcatt atgtgagctt ccggacattt gttgtaagat cttccctaaa gaaaataatg
     1021 attttttata catagtt
//
LOCUS       DAZFDC010000386         1034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFDC010000386 DAZFDC010000000
VERSION     DAZFDC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.389"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..66
                     /locus_tag="ACHJ48_005181"
     CDS             <1..66
                     /locus_tag="ACHJ48_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710490.1"
                     /translation="VRGQLVPGERLGIKITELECA"
     gene            63..752
                     /locus_tag="ACHJ48_005182"
     CDS             63..752
                     /locus_tag="ACHJ48_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO4710491.1"
                     /translation="MNNHYLQFVRELLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYKELALLVGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            963..1034
                     /locus_tag="ACHJ48_005183"
     tRNA            963..1034
                     /locus_tag="ACHJ48_005183"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:996..998,aa:Ile,seq:cat)
BASE COUNT          293 a          203 c          281 g          257 t
ORIGIN      
        1 gtgcgcggtc agctcgttcc tggtgagcgg ctggggataa aaatcacaga actggagtgc
       61 gcatgaataa ccactattta cagtttgtgc gtgagctgct cattatcgct accgccgatt
      121 tgagtggggc aacaaaaggt cagcttgaag cctggcagga gaatgccatg ttcgatacag
      181 ggcgttacag gcgaaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      241 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggtgacgtca attcctctgg
      301 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagaac
      361 atcataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
      421 cgataacaca gtgggcgtgg aatgaattta atactcaatc cggtaccaga aaaattgcag
      481 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
      541 aactttttgg tggggaaggt tatgaataca aggagctggc attactggtg ggagtgacaa
      601 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
      661 gactggatag tgaggcttta ttgtttgtaa tgagaacgcg ttcaaaacaa aaggcggcat
      721 tttcaaagca aagtgttgca aaagtagatt gaaaggcata tatttcatgc aaatctgata
      781 ttttgccgat tttgtacgtg atggcaaaag caaacaaaac ccgcccacaa gcgggttttt
      841 ttgtgccact tatctcggat agacatggtg aatgcgctgg tggaggaagt aagggtaatt
      901 tttaaccagg tgattcttga atgcttgcaa cattgatttc gtaacgttat tatcctgcgc
      961 ccggcccttt agctcagtgg tgagagcgag cgactcataa tcgccaggcc gctggttcaa
     1021 atccagcaag ggcc
//
LOCUS       DAZFDC010000387         1025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFDC010000387 DAZFDC010000000
VERSION     DAZFDC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.390"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..711)
                     /locus_tag="ACHJ48_005184"
     CDS             complement(<1..711)
                     /locus_tag="ACHJ48_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4710492.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIC"
     gene            complement(717..>1025)
                     /locus_tag="ACHJ48_005185"
     CDS             complement(717..>1025)
                     /locus_tag="ACHJ48_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4710493.1"
                     /translation="VISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCT
                     WTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ
                     "
BASE COUNT          227 a          326 c          270 g          202 t
ORIGIN      
        1 gcaaatcccc gtaaaggcag atgcgcgcca tgcccggtga cgccagaggg agtgtgtgcg
       61 tcgctgccat ttgtcggtat acctctctcg tttgctcagt tgttcaggaa tatggtgcag
      121 cagctcgcca tcaccacagt aaatggcggc atgattcggc accgatgaac caaaacagca
      181 cagcagcaca tcgcccggtt gtgctgatga caacggcacc tgatacagcc ctgtggcctc
      241 cagattatcc agatagagat tctgaccgtg acgccaccag tcatcctcgc gatgaaaatc
      301 cggcatctca atccccgcca gatgataagc atcccggaac agcgtgtaac agtccgtcac
      361 cccgtgctca aagcgccgcc cggtgagatg cggcacacag cggaacttat gaatcgtccc
      421 ccggcagacc agccaccacg gcaaatcact ctgcacctgc agccgccggt cggcctcact
      481 cagccagggc agaccaccgg ggtggctgtg gaccagcgcc acaatctcac cctgcatttc
      541 tgcctgcagc cagtcttccg gcgacatacg gaaatacgcc tccggctcac cggagatatt
      601 cacgcagggg aaatatcttt ccccctccgg cgtgcttacc acgaagccgc acgactccgc
      661 tggcgcacat cgccgggcgt gcgccagaat cgctgattct gtctgtgtca tgggatttac
      721 tgcgaaagtt tgttaatgga aaggaagccg ccaaagttgc cgacgttatt gcggaactta
      781 caaccgctca ggcatttgct gcatttatcc ttcgtgatat cggacgttgg ctggtcatat
      841 tcatccgcga ccgccggacc gtgataaccg cactcatcgc cgcgataggt ccaggtgcag
      901 gtgttggcca gcatgatacg tcccggaaaa acagcgccgt ccgtttccgt cggcgtggac
      961 agtacaaaag aggcactcac cgcgctcagt tcgctgcact gctcaatgcg ccagcggctg
     1021 atcac
//
LOCUS       DAZFDC010000388         1024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFDC010000388 DAZFDC010000000
VERSION     DAZFDC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.391"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..232
                     /locus_tag="ACHJ48_005186"
     CDS             <1..232
                     /locus_tag="ACHJ48_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO4710494.1"
                     /translation="KTTIPNLTKNNPGKNLDKIHPGDILHYQKASMKVIITGWKPITI
                     KNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            235..684
                     /locus_tag="ACHJ48_005187"
     CDS             235..684
                     /locus_tag="ACHJ48_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFO4710495.1"
                     /translation="MRKITIILLLTFSCTKGLAFDISMSKECSGVDELEFIDCVKNSF
                     IMSTITLKKAEANIKEKIQKWNSDEDEAIRKEAVISLKRLESSNREFIRYRFAQCSFT
                     LTWGARVGRLAFSRLYPCYTELNKIRAMQLNNAYISDFQNPDEISSK"
BASE COUNT          403 a          161 c          162 g          298 t
ORIGIN      
        1 aaaaacgacc atacctaact taacgaaaaa caatcctgga aaaaatctcg ataaaataca
       61 cccgggagat attttacatt atcaaaaagc atccatgaaa gtaataatta ctggatggaa
      121 acctatcaca ataaaaaatg tagcgatgaa ttataatggt ggtggcgatc ccaagtatgc
      181 aataaaactt cagttcgttt atacattact cacaaaaaac agagtattat gagaatgcga
      241 aaaataacaa ttattctact gctgacattc tcatgtacaa aaggtctggc atttgacatt
      301 tccatgtcta aagaatgtag tggtgttgat gaacttgaat ttattgattg tgttaaaaac
      361 tcatttatta tgagcactat tacacttaaa aaagcagaag caaatataaa agagaaaatt
      421 caaaaatgga atagtgatga ggacgaagca ataagaaaag aagcagttat ttctttaaaa
      481 agactagagt cttctaatag ggaatttata cgttacagat ttgctcaatg ctcttttact
      541 ctaacctggg gggccagagt agggcgactg gcattctctc ggctatatcc atgctatact
      601 gagttaaaca aaataagagc catgcaacta aacaatgctt acatttctga ctttcaaaat
      661 ccagatgaga tatccagcaa atgattatta taaatttaag attaacatta ctatttatac
      721 tggccacatt tacatattca gtacaatcag acgaattcga tgatcaaacc acttacacgc
      781 aataccgtta tttttccact gctataccgg aagatatacg tagatttatg ttctacgtag
      841 attattgctt tgtagattta gcattcgata tcgaaactga ctcaatgaca gctaagaatg
      901 atattattaa tgaatgtact ccattaaaag aaagaaacaa gacattaaaa gaaaaatata
      961 aaaaaatgaa gagataatag caattctttc cattgcagat gatactattt taatttatga
     1021 agaa
//
LOCUS       DAZFDC010000389         1012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFDC010000389 DAZFDC010000000
VERSION     DAZFDC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.392"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            262..>1012
                     /locus_tag="ACHJ48_005188"
     CDS             262..>1012
                     /locus_tag="ACHJ48_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710496.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGD"
BASE COUNT          369 a          193 c          172 g          278 t
ORIGIN      
        1 catttcacac actatcggag aacatcataa ctttttaaaa tgagcgcttt ttgatagttg
       61 ttaacgtttt cctttacgat actatcaatg ccctttttta tacacgaata ttaccgggtg
      121 gagataaaag ggaagtcaaa ggtaattata tcaggaagat atgacgcatg atatttattg
      181 tgcgtagaag aaaatccatt cctgctttaa ttatccaatt taaaaatctt agcataaaac
      241 agataatgaa taaggaatgt tatgtcaatt gactttaccc caggtatgat aaatacatat
      301 cacggcgata tttataaccg cacaacagat accgacaatg ttaaaacacc agacacaccg
      361 atgtggcctt gtgataatcg ggaagaacaa caacccatta attccacatt ctctggagaa
      421 ggatacaacc ctgaacaata tgatttagcg cggcaacacc tcccacaaat ttatgcgtgc
      481 cgcacgaaca ccaaatatac agatgcagac tactcaaaag ttgtggctca acttgtaagt
      541 cttattacta acattgaatc gataagttcg acaccactta cgcggcaggc acaggaaata
      601 cttaatcaaa ttaataacat tagatacgaa aagaacaaaa gtgctgaatg tcgaatcatt
      661 gttgtcgcta atcctaaacc cgacaaggca atcataacta aaattagcgt agaagaggga
      721 ataccagtaa gattcagtgt ccaaaccatg ttttcagata ctaattttat tgctgaacaa
      781 cgagctgact taccgactaa tataaaagat attcaaagcc tgtatcaaaa aatgacgaag
      841 ttatatattg agcacagtga aaataaaaac aggatgaaag tctttgccgg aaccaacttt
      901 attgatttca atatgacagg acagaacctg tccgggttcg ttctaacctt gtcaaggttt
      961 tatttcgaag atctacttaa tataaatttc actgacgcaa atttaggtga ca
//
LOCUS       DAZFDC010000390          994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFDC010000390 DAZFDC010000000
VERSION     DAZFDC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.393"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            77..>994
                     /gene="phoE"
                     /locus_tag="ACHJ48_005189"
     CDS             77..>994
                     /gene="phoE"
                     /locus_tag="ACHJ48_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO4710497.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYI"
BASE COUNT          292 a          210 c          252 g          240 t
ORIGIN      
        1 caggttaaaa actttcctgt tttcaacggg gctctcccgc agaatattcg cgcgttaatt
       61 aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc
      121 atctgcatcc gtacaggccg cagaaatata taacaaagac ggtaataaac tggatgtcta
      181 tggcaaagtt aaagccatgc attatatgag tgataacgac agtaaagatg gcgaccagag
      241 ttatatccgt tttggtttta aaggcgaaac acaaattaac gatcaactga ctggttatgg
      301 tcgttgggaa gcggagtttg ccggaaataa agcggagagt gatactgcac agcaaaaaac
      361 gcgtctcgct tttgccggat tgaagtataa agatttgggt tctttcgact atggccgtaa
      421 cctgggcgcg ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga
      481 ctcctcggcg cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg
      541 gaacaccgac ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa
      601 aaacgaaaac cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata
      661 tgactttggc ggcagcgatt tcgccattag tggggcctat accaactcag atcgcaccaa
      721 cgagcagaac ctgcaaagcc gtggcacagg caagcgtgca gaagcttggg ctacaggtct
      781 gaaatacgat gccaataata tttatctggc aactttttat tctgaaacac gcaaaatgac
      841 gccaataact ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca
      901 gtttgacttt ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga
      961 aggtatcggt gatgaagatc tggtcaatta tatc
//
LOCUS       DAZFDC010000391          949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFDC010000391 DAZFDC010000000
VERSION     DAZFDC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.394"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>949
                     /locus_tag="ACHJ48_005190"
     CDS             <1..>949
                     /locus_tag="ACHJ48_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFO4710498.1"
                     /translation="PSITTIIKKTEYYGHQLTNAMTVASVVKPYDFSNLGAINSIDKS
                     VSDVPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSK
                     GKCLGLSLLYLDTGGYGGGYQKLRHNIDTASTLYQTKYNDNLKLSNRDDFFLRKTQRI
                     ITMSNELGNNRLKNAQLEVLELKDPILTEGILYQRRISSLLITTEYHSLALQQISSFW
                     RVTDPNFGHCDFHSLAQALTFIKNITSNRNFSSLYGSGIVKIYFSESLNNWKYIKLPL
                     VQTGSLLRDIYLTTPEKLSTSGGSLNIMGHLVPVSFIYDI"
BASE COUNT          329 a          164 c          155 g          301 t
ORIGIN      
        1 tcccagcata acaacaataa taaaaaaaac agaatattat gggcatcagt taacaaatgc
       61 tatgactgtt gcttcagtgg ttaaaccata tgactttagc aatcttggtg ccataaatag
      121 tatagataaa tccgtttctg atgtgcctgc attgcataca attgttgaac aagcaaaata
      181 taatttactt tcatggaatg atttttataa cactcatgcg tcaatatggg atacaatagc
      241 aagacagcat aaaagtacaa atatcgaatt tcacccacaa agtcttcttt ttgatagaga
      301 ttcgaaagga aagtgccttg gtttatctct tctgtatctt gatacagggg ggtacggagg
      361 cggatatcaa aaactgaggc acaatattga cactgccagc acattatatc aaacaaaata
      421 caatgacaat ctaaaattat ctaaccgtga tgatttcttt ttaagaaaaa cacagcgtat
      481 cattacaatg tccaatgagt taggtaataa caggttaaaa aatgcacagt tagaggttct
      541 ggaattgaaa gaccctatac taactgaagg catattatac cagcgtcgta tatcatctct
      601 tttaataaca acagaatatc attcacttgc attacaacaa atatcttctt tttggagagt
      661 aactgatcct aattttggtc attgtgactt tcattcctta gcacaggctt taacgtttat
      721 aaaaaatata acgagcaatc gtaacttttc atctttatat ggaagtggca ttgttaaaat
      781 ttacttcagt gaatctctta ataattggaa gtatattaag ctcccccttg ttcagactgg
      841 ttctttgtta agagacatat atttaacaac cccagaaaaa ttatccacaa gcgggggttc
      901 attaaatatt atggggcatc tcgtgcctgt cagttttatt tatgatatc
//
LOCUS       DAZFDC010000392          936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFDC010000392 DAZFDC010000000
VERSION     DAZFDC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.395"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..820
                     /gene="yeeO"
                     /locus_tag="ACHJ48_005191"
     CDS             <1..820
                     /gene="yeeO"
                     /locus_tag="ACHJ48_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO4710499.1"
                     /translation="VGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFS
                     IIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSAL
                     GSASTIITGRRLGVGQIAQAEIQLRHVFWLTTLGLTAIAWLTAPFAGVMASFYTQDPQ
                     VKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLG
                     IMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
BASE COUNT          189 a          184 c          269 g          294 t
ORIGIN      
        1 tgtcggggct gggttgggtt taaccatttc tcgttatatt ggcgcagttg caattttgtg
       61 ggtgctggcg attggtttta atcctgcgct aaggatttcg ttaaagagct attttaaacc
      121 gctgaatttt agcattatct gggaagtcat ggggattggt attccagcga gtgtcgaatc
      181 agtgttattt accagtggtc ggttattaac ccaaatgttc gttgccggaa tggggaccag
      241 tgttattgcc ggaaatttta tcgcgttttc aattgcggct cttatcaact tacccggaag
      301 tgcgctcggt tctgcttcta cgatcattac aggccgaagg ttgggggtag ggcagatagc
      361 gcaagcagag attcagttgc ggcatgtgtt ttggctgacc actcttggat taacggccat
      421 cgcctggcta acggctccct ttgccggggt tatggcatcg ttttacaccc aggatccaca
      481 ggttaaacat gtcgttgtga ttctgatttg gctaaatgct ttatttatgc ctatttggtc
      541 cgcttcatgg gtgctacccg ctggatttaa gggggctcgt gacgcccgtt acgctatgtg
      601 ggtttctatg ttgagcatgt ggggttgtcg ggttgtcgtc ggttatgtgc tgggcatcat
      661 gcttggctgg ggtgtggttg gtgtctggat gggaatgttt gccgactggg ctgtgcgggc
      721 cgtgctgttt tactggcgaa tggttactgg acgttggctg tggaaatacc ctcgacccga
      781 accgcaaaag tgtgaaaaaa agccagctgt gtcggaataa acgacaaaat gcagattatt
      841 tcagcaaacg atttcaaatt taaaaaacag gctttgacat tgtgggtggg catcgctaat
      901 attcgcctcg ttctcacgat tcctctgtag ttcagt
//
LOCUS       DAZFDC010000393          927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFDC010000393 DAZFDC010000000
VERSION     DAZFDC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.396"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            263..669
                     /gene="ipbA"
                     /locus_tag="ACHJ48_005192"
                     /gene_synonym="fimX"
                     /pseudo
     CDS             263..669
                     /gene="ipbA"
                     /locus_tag="ACHJ48_005192"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="IpbA is also known as HbiF and FimX; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
BASE COUNT          292 a          186 c          176 g          273 t
ORIGIN      
        1 cacatcaatg tagataaaaa cacgcattaa aaatggcttg tagaaaagca gcacaataaa
       61 tagttcatat ttagtctatt gcgtagaact actattcaaa aaatcttatt aatgagataa
      121 tgagattaca tcgataacgt tctgattgca ggcatactta tctgggtgtg cctctctttt
      181 ttacaactgt acacggtggc gtatgacgag aaaatatctc acacaggatg aagtctacag
      241 gctgatggat gcagcccaga gcatgtcctt tcctgaaaga aatcgctgtc taattatgat
      301 ggctttcatt cacggtttta gggccagtga acttcttgat ttacgtttat ccgatattga
      361 tgcatcagga aaacaactta atattcggcg gattaaaaat ggtttctcga caacacatcc
      421 tcttcttcct gatgaatata atttgatcaa gttgtggctt aagcagagga agttaattga
      481 aaacggagtt gaaggagact ggctttttct ctcccggaaa cgccgcccta tcagcagaca
      541 acattttttt ctatcattcg tgaggctgga agacgtgcag gattagctgt aaaagcacat
      601 cctcatatgt tacgtcatgc ctgtggtttt gctctggctg acaatggtgt cgatacccga
      661 ctacttcagg attatctggg tcatcgaaat atccagcata ctgtcagata cacagccagt
      721 aatgctgctc gatttaaagg ggtgtggaaa aaaaagcctc gttaaaacag taccttctga
      781 ttcaaaatgt cagattccta cgttttcctc aattcttcca taaaaaaaca gcccattcaa
      841 ttcaataaaa cacataacat gttgatattt ctcgctaaag taaaataacc ccatgatttg
      901 aaatgattaa ccgccaaggt catacca
//
LOCUS       DAZFDC010000394          922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFDC010000394 DAZFDC010000000
VERSION     DAZFDC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.397"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..824
                     /gene="espX1"
                     /locus_tag="ACHJ48_005193"
     CDS             <1..824
                     /gene="espX1"
                     /locus_tag="ACHJ48_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO4710500.1"
                     /translation="CNKIPASSTDDNQKITWQNFKTSNAAYSQLCEKLSLLKSSPSRL
                     IEKSAYCSNENMITDKFDVVFSYCGDNVKEFILLLPYNKSLEMHELNEQNIQYLTALN
                     INIHKLLLSNITIEKSNLSYGYYFGCVLSNISCFESDLSNTIFSNGEINNLFIKKSNI
                     FGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIP
                     LVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLQSINESMFLMKKISHQD"
BASE COUNT          359 a          164 c          139 g          260 t
ORIGIN      
        1 aatgcaataa aatccccgcc tcatcgacag atgataacca gaaaataaca tggcagaatt
       61 ttaaaactag caatgcggca tattcacaac tatgtgaaaa attatcttta ttaaaaagct
      121 cgcccagtcg gttaatagag aaatcagctt actgctcaaa tgaaaacatg atcaccgata
      181 aatttgatgt tgtcttttct tactgcggag ataatgtaaa ggaatttata cttttattac
      241 cctataataa atcgctggag atgcatgaac ttaacgagca aaacatccag tatttgactg
      301 cccttaatat taatatacat aaactgttgc tatcaaatat aacaatagaa aagagcaacc
      361 tttcatatgg gtattatttt ggttgtgtat tatccaatat atcatgtttt gaaagcgact
      421 tatcaaacac catcttttca aatggagaaa taaacaacct cttcattaaa aaaagcaaca
      481 tatttggaac atcgttcact aataccatga taaaaaattt gcgctgtgaa gatattatgc
      541 ctggccgatg gaccactcaa cttgtcaata agcacttagg ttacagatac acaggagtat
      601 tcaaaaccct ggcaagtatt gatgacaaac cgtcccgttt cgaaattttg ataccattgg
      661 tacaaacttt agttcgcgac aatgtaaagc taaataatga tgtgtataaa gaactgaaaa
      721 agtttatgca cgattacgac aaaacatccc cagaaatgag aaaatacctt cagagcatca
      781 acgagtctat gttccttatg aaaaaaatat ctcatcaaga ttaaaacacc ccaaaaaaga
      841 agtaatttca gaatgaaatt acctgtgata attattaatt aactgcagag aaataaaaaa
      901 taatctgaag catgctctga aa
//
LOCUS       DAZFDC010000395          920 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFDC010000395 DAZFDC010000000
VERSION     DAZFDC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.398"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            51..851
                     /locus_tag="ACHJ48_005194"
     CDS             51..851
                     /locus_tag="ACHJ48_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710501.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPTGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
BASE COUNT          294 a          178 c          207 g          241 t
ORIGIN      
        1 atcaatgcta aatattctaa tcattatgac aggcgaggga gtgtccaatt atgaattcaa
       61 aaaagctttg ttgcatatgt gtgttattct cgctgcttgc aggatgtgcc tctgaatctt
      121 ctattgatga aaagaagaaa aaagctcaag tcacacaaag taatattaat aaaaacactc
      181 cccagcaact gacagacaaa gatttattcg gtaatgaaac cactctggcc gtatccgaag
      241 aggatattca agcagcgctt gatggagatg agttccgcgt tccccttaat tctcctgtaa
      301 tacttgttca atccggcaac cgcgcaccgg aaaccattat gcaggaggag atgcgaaaat
      361 attatactgt ttccacattc tctggtatcc cggacaggca aaagcctctg acttgtaaca
      421 aaaacaaaga taaaaatgag aacgaggatg ttgcccgtgc tgagaatatg aactggatgc
      481 aggcactgcg ttttgtggct gcaaaaggac atcagaaagc gattattgtt taccaggata
      541 tgttgcagac aggaaaatat gactctgcgc tgaaatcaac agtatggtcc gactataaaa
      601 atgaaaaact cactgacgct atctccctgc gctacctggt acgtttcacg ctggtggatg
      661 tggcaacagg tgagtgggct acctggtcgc cggtgaatta tgaatcgaga gtaatttttc
      721 cgccgactgg aataacaaaa acgagcaata aagaactatc aaatgaccat gttacggaag
      781 cgcaactgtt taatttgaaa caaaaaacat atgtatcgat ggtgaaagac ttggtgagtc
      841 gattccagta atcgttattg ttatgctgcc gcctgtagtt tagggtggca gcaaacagga
      901 gttgagtatt tctataatgt
//
LOCUS       DAZFDC010000396          918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFDC010000396 DAZFDC010000000
VERSION     DAZFDC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.399"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(103..675)
                     /locus_tag="ACHJ48_005195"
     CDS             complement(103..675)
                     /locus_tag="ACHJ48_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4710502.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGQGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPEKGVFVKNSDGSDVCTLFDAAAFSRLTGEDLPHPL
                     TREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            complement(708..785)
                     /locus_tag="ACHJ48_005196"
                     /pseudo
     CDS             complement(708..785)
                     /locus_tag="ACHJ48_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(787..>918)
                     /locus_tag="ACHJ48_005197"
     CDS             complement(787..>918)
                     /locus_tag="ACHJ48_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4710503.1"
                     /translation="PEQVRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          261 a          244 c          154 g          259 t
ORIGIN      
        1 acttgggata cttaaggtgg taacaggcat aaaaacctca ttcagtaatt catatcagtt
       61 tgcaggatgt ttcattaaat aaaataaagg gtaatgttca tttcaattac cctttataac
      121 gaagtttcct ctggtatcgt cataaatgca ttcttcatgt tttacaatta ttgatgccgt
      181 tattggttcc cgggtcagtg ggtggggtaa gtcttcacca gtcaaacgag aaaatgcagc
      241 ggcatcaaat aaagtacata catctgaacc atctgaattc ttcacaaaaa cacctttttc
      301 aggctgctcc agtgtaattg gacattgaag cgcatcacca ggacattgaa gtttttcaat
      361 atcgacagta aaggcacact ccgatatttt ctcctgcaaa gagtttcgcc ctcccattcc
      421 agggagcgca cttaatatcc tgtttctgag cacattaact ccatcattaa aagcaatatt
      481 caataattct gcataaaccc gtccctgccc ttcactttct gctaaaaaac ggcctgtatc
      541 tggcatataa gaaactctaa ccctctcccc tcccaggaga aatccaccaa tctgcccctc
      601 cattacctgc tgacgcagct ccatcatctc attcacgaat gaagccgatg acacatatga
      661 tattaatcca ggcattacca tgctccttca tacggataaa tattccctca agtcttcatt
      721 aacaaacatc caccggacat gacaacaaaa accggagccg gactccggtt ttgtgaagct
      781 gtcgggttac ttcatcccgc caatattttc ccacgtcccg tcagcacgca ggatttgcag
      841 cggtcttacc acacactgta tctgcttttt atccgcatcc agtatcacca cctgcgtgat
      901 taccctgacc tgctccgg
//
LOCUS       DAZFDC010000397          911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFDC010000397 DAZFDC010000000
VERSION     DAZFDC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.400"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(39..775)
                     /locus_tag="ACHJ48_005198"
                     /pseudo
     CDS             complement(39..775)
                     /locus_tag="ACHJ48_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          241 a          194 c          205 g          271 t
ORIGIN      
        1 tttacttttc ccttatcgct gtcgattctc attatcattc atggcaaaaa aacagcaaaa
       61 gaggccctgg taatgactcc tgtcagtagt acatgtgttt tcgataaatg aaccaatggc
      121 ttttcgtgaa tttccaggga gcgtaaaaag caaatcgttt tccgggcagg tcgtttgatg
      181 tgagtacgaa gtgtcagatt gttgcgctca atgcgccggg taaatatctt gccgacaaga
      241 tgcttatcct gtggcatttc tctggtataa ctgctccggt tgtctctggt tatcatgcct
      301 gcggagaatg gcttcaggaa ttccggcaac tcacggcatg tttcatcagt gcgaggacca
      361 aaagtgtaag ccagcacacc gtcagctttg gtcttatacg cgtaccagtg ccactgctga
      421 cgagctttgt tttcgacaaa actccattgt tcatcaagtt cacagataag tgccacatcg
      481 acactggcgg ctggagatat ggttacttta cgtggtgagc gttttttaaa gtgagaatga
      541 cggtgttgat atccaccttc agcgttctgg agctatcgcg aaccccggct ccgttatgaa
      601 ccatttcaac aatttgctct ttaatgcctg gttttcgggt ttcgtagatg taattcagtt
      661 gaaatacacg tttgcaggat tagcactgga aacgctcatg atctgaggtg ctgtgatcat
      721 aacggtacac tttataggaa ttacagcggg gagaatatac tgtaactgtt gccatatggt
      781 ctccagatac caatagaata caacattaat ctatcgtcag aaggcatcac cggggccttc
      841 tgacataatc tgttaatacg tgtactatta ccctgttcag aaaatattga ttcaaaaatg
      901 aaaaccagtt a
//
LOCUS       DAZFDC010000398          910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFDC010000398 DAZFDC010000000
VERSION     DAZFDC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.401"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..143)
                     /locus_tag="ACHJ48_005199"
     CDS             complement(<1..143)
                     /locus_tag="ACHJ48_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO4710504.1"
                     /translation="MFPATKGALMRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIAL
                     ERR"
     gene            complement(91..657)
                     /locus_tag="ACHJ48_005200"
     CDS             complement(91..657)
                     /locus_tag="ACHJ48_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO4710505.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGAFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          281 a          204 c          143 g          282 t
ORIGIN      
        1 cttcgtcgtt cgagtgctat cttggttcca caaatctcat tacaccaata ttgattttga
       61 tattttggta taaaccattc attgcaacat ttacatttcc ttcgatagat tcgcataagt
      121 gctcctttcg ttgccggaaa aatcaccgta atacttatct cgggcttctt cagcaactag
      181 taccgctaac tccagatcat caaagcatcc gaagtgttta ctcttgccat ggaatcctag
      241 cctaacattc cattttttct gtcgtttgtg ccaagtaacc cctctgcaac ctgatttgct
      301 attctttcgg atccttatat ttcttgaatt ttctattggc aggcattctc ttaaattttc
      361 tggcctattg tcggtcctaa ttccattaac gtggtcaatt tgaccagcag gccaacgatt
      421 atgagttatg taaaaaacta agacgtgagt tttatatcta cgcccatcta tcatgatcat
      481 tgaataaccg ttggaatcaa aagctccagc aacactattt aatgctatcc ttccctgagt
      541 gggaactttc catctaaata ccccggtaga tttatcgaaa cttagtaact caaatatcct
      601 tttaacagtt aaatcttctc ttttacggtt acatcgtctt cgcgctggtt tagccatcgc
      661 cttcttcctc cgtaatggtt ttctgaattt ggccacctga acagagctca ccaaagctat
      721 ggatgtcggt atttccacaa taccaagatg gcgaaaataa ctgcatgata agcctcaggg
      781 aaaagggaag acactacccc cgataattca gaaacaaatc gaaatacatg aactaaagaa
      841 aaaatcacaa caaatagaaa tagaaataga aatagaaata gaaatagaaa tagaaatatt
      901 aaaacagacc
//
LOCUS       DAZFDC010000399          908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFDC010000399 DAZFDC010000000
VERSION     DAZFDC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.402"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..510
                     /locus_tag="ACHJ48_005201"
     CDS             <1..510
                     /locus_tag="ACHJ48_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4710506.1"
                     /translation="KTASSVARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPA
                     IKLGEARVRLSRRKRRKKGQRAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMP
                     WASSSTGADSKGRPKRHRLPIEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTH
                     QLRLVLKRK"
     gene            523..>908
                     /gene="gpU"
                     /locus_tag="ACHJ48_005202"
     CDS             523..>908
                     /gene="gpU"
                     /locus_tag="ACHJ48_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO4710507.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
BASE COUNT          221 a          214 c          282 g          191 t
ORIGIN      
        1 aaaacggcct cttcagttgc cagggagctg gccgttcccc gtcgtcttat ccgtgcccgc
       61 atccggttaa gtccggcacg accggataag gtttacgcaa aggtttacat caataccggc
      121 aacctgcccg ccatcaaact gggggaggcc cgcgttcgac tttcccgcag aaaacggaga
      181 aagaaaggac agcgtgcggc cctgaaaggg ggcggcagtg tgctgattgt ggggaaaaga
      241 cggatcccgg acgcctttat cacccggctg gctaacggac gctggcatgt gatgcagcgt
      301 atgccgtggg catcatcatc caccggcgcg gacagcaaag ggaggccgaa acgccaccgt
      361 ctgccgatcg aagtggtgaa gattacgact gccggaccgc tggcagaaac ctttgaacgt
      421 gaacgggacc ggatgtaccg ggaaaaatta ccggcgcaga tgatgaaagc catgacgcat
      481 cagttacgcc tggtgctgaa aagaaaatga ctgggagggt gtatgaaaca ccgtgaaata
      541 cgggcggcag ttctgtctgc cctgaaagaa aatatttctg agagggtgag ctggtttgac
      601 ggtcgcccgg tttttattga tgaacaggaa ctgcctgctg ttgctgttta cctgacagat
      661 gcgtctgctg ctgacgagtt cgttgatgag ggaacctggg aggcgacact gcatattgag
      721 gtttttctca gggcaaaaga accggactcg gcactggata tgtggatgga agaaaaaatt
      781 cttcctgcgc tggaggcagt tcccgggctc agtgcattac tgctgaagat gaatcttcag
      841 gggtatgact accgccggga tgatgagttt atgatgtggg gatcggcaga tctcctgtgg
      901 aaaattac
//
LOCUS       DAZFDC010000400          903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFDC010000400 DAZFDC010000000
VERSION     DAZFDC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.403"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            60..>903
                     /locus_tag="ACHJ48_005203"
     CDS             60..>903
                     /locus_tag="ACHJ48_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFO4710508.1"
                     /translation="MGTAAISHLRYDLNKYALSLRKTATLASTFFIESPLVRFEYLQE
                     IENTINDITHRFNSSYDINEKARLINELKMESETARKEYQLFRQGNYDKYITTDIFEE
                     HGLIKYVNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAHGANNFYESLS
                     PLFFNEYDSGPIRELYRVIAKKMGGDINSADYAYSIVDFSITAYGGYSGMKIVPKYNR
                     LIRPSLGNRPGTGRLFHYTSVDFKNKFSLKPTPLKIIQISSTVKKFKVTFYDENTNLR
                     IKHGL"
BASE COUNT          321 a          138 c          158 g          286 t
ORIGIN      
        1 gaacatattt tcgcaagcaa tagttattac tgtttgttta tggatagaga gtctacttaa
       61 tgggtacagc agctataagt catttacgat atgatcttaa caaatatgcc ctttctttac
      121 gcaaaaccgc aacacttgct tcaacatttt ttattgagtc cccattagtt agattcgagt
      181 atctgcaaga aatagaaaat actattaatg atattactca cagattcaat tcttcatatg
      241 acatcaatga aaaagcacga cttatcaatg aattaaaaat ggagtcagaa acagcaagaa
      301 aagaatatca acttttcaga caaggaaatt atgataaata tatcactact gatattttcg
      361 aagaacatgg tttgataaaa tatgtgaact taagtttcga tattgttgca tctgtaggac
      421 aggtcgtagg cggggttggc gcgttgaaat ttggtaaggt agtgcattca aaccgaataa
      481 aaggtatagg cgtcacatta gttgctcatg gggcgaataa tttttatgaa agcttatcac
      541 ctttattttt taacgagtat gattcaggtc caatacgtga actctacaga gtaatagcaa
      601 aaaaaatggg aggtgatatt aattctgctg attatgcata cagcatcgtg gacttttcga
      661 ttactgcata tggtggatat tcaggcatga aaatcgtacc taagtataat agattgattc
      721 gtccttcatt aggaaataga cctggtacag gacgattatt tcattacaca tcagttgatt
      781 ttaaaaataa attctcatta aaacctaccc ctttaaaaat catacaaatt agttctactg
      841 taaaaaaatt caaggttaca ttttatgatg aaaatacaaa tttgaggata aaacatggcc
      901 tca
//
LOCUS       DAZFDC010000401          893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFDC010000401 DAZFDC010000000
VERSION     DAZFDC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.404"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..397
                     /gene="ler"
                     /locus_tag="ACHJ48_005204"
     CDS             26..397
                     /gene="ler"
                     /locus_tag="ACHJ48_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO4710509.1"
                     /translation="MNMENNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEEIIPLKNI"
     gene            412..630
                     /gene="escE"
                     /locus_tag="ACHJ48_005205"
     CDS             412..630
                     /gene="escE"
                     /locus_tag="ACHJ48_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFO4710510.1"
                     /translation="MITITELEDEIIKNKEAANVFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRTIEIMRIRINNK"
     gene            643..>893
                     /gene="cesAB"
                     /locus_tag="ACHJ48_005206"
     CDS             643..>893
                     /gene="cesAB"
                     /locus_tag="ACHJ48_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesAB"
                     /protein_id="HFO4710511.1"
                     /translation="MSQTRNKELLDKKIRSEIEAIKKIIAEFDVVKESVNELSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETLSP"
BASE COUNT          372 a          128 c          191 g          202 t
ORIGIN      
        1 ttaaagcatg cggagattat ttattatgaa tatggaaaat aattcacata caacaagtcc
       61 atacattcag cttatagagc aaattgcagt tctacagcag gaagcaaagc gactgcgaga
      121 gcaggaagtt caaagtgtaa ttgagtcgat tcagaagcag attacttatt acaatataac
      181 cttacaagag ctgggatata ctaatgtgcc tgatgatgga ctcgctcgcc ggaactcatc
      241 gaaaggtgtt tactaccgca atgaagaagg gcagacctgg tcgggcgtag gccgacagcc
      301 acgctggctt aaagaagcac tgttgaatgg aatgaagaaa gaagattttc ttgtgaagga
      361 cactgaagaa gaaataatac cgctgaaaaa tatttaacat gaaataatta aatgataacg
      421 ataactgagc tggaagatga aataataaaa aataaagaag ccgcaaatgt ttttattgaa
      481 aaaataaacg acaaaaagaa cgaaatccat gaaaaaatga aacacccttt ggataaagtt
      541 acctacaatg aagcaaagga actgctcatt gcgtgtgatg cggcaattag gactatagag
      601 ataatgcgaa tcaggattaa taataaatag aggatgagta ttgtgagcca aacaagaaat
      661 aaagaactat tagataaaaa aataagatca gaaattgagg cgataaaaaa aataatcgcc
      721 gaatttgatg tcgtaaaaga aagcgttaat gagttaagtg aaaaagcaaa aacagatcct
      781 caggcggcag aaaaattaaa caaattaatt gaggggtaca catatggtga agagcgaaaa
      841 ttatatgatt ctgccctaag taagattgaa aaactaatag agacactgag tcc
//
LOCUS       DAZFDC010000402          889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFDC010000402 DAZFDC010000000
VERSION     DAZFDC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.405"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..465
                     /locus_tag="ACHJ48_005207"
     CDS             <1..465
                     /locus_tag="ACHJ48_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO4710512.1"
                     /translation="PIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTL
                     TPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALD
                     CFVYALAALRISISRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            462..668
                     /locus_tag="ACHJ48_005208"
     CDS             462..668
                     /locus_tag="ACHJ48_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO4710513.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            665..>889
                     /locus_tag="ACHJ48_005209"
     CDS             665..>889
                     /locus_tag="ACHJ48_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO4710514.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQ"
BASE COUNT          230 a          231 c          266 g          162 t
ORIGIN      
        1 cccattaaag gggcatccgt ctacggaaag ccggtggcca gcatgccacg taagcgaaac
       61 aaaaacgggg tttaccttac cgaaatcggt acggataccg cgaaagagca gatttataac
      121 cgcttcacac tgacgccgga aggggatgaa ccgcttcccg gtgccgttca cttcccgaat
      181 aacccggata tttttgatct gaccgaagcg cagcaactga ctgctgaaga gcaggtcgaa
      241 aaatgggtgg atggcaggaa aaaaatactg tgggacagca aaaagcgacg caatgaggcg
      301 ctcgactgct tcgtttatgc gctggcggcg ctgcgcatca gtatttcccg ctggcagctg
      361 gatctcagtg cactgctggc gagcctgcag gaagaggatg gtgcagcaac caacaagaaa
      421 acactggcag aatacgcccg tgccttatcc ggagaggatg aatgacgcga caggaagaac
      481 ttgccgctgc ccgtgcggca ctgcatgacc tgatgacagg aaaacgggtg gcaacggtac
      541 agaaagacgg acggcgagtg gagtttacga ccacttccgt gtctgacctg aaaaaataca
      601 ttgctgagct ggaagtgcag accggcatga cacagcgacg caggggacct gcaggatttt
      661 atgtatgaaa atgtccacca ttcccaccct tctggggccg gacggcatga catcgctgcg
      721 tgaatatgcc ggttatcacg gcggtggcag cggatttggt gggcagttgc gggcgtggaa
      781 cccaccgggt gaaagtgtgg atgcagcctt gctgcccaac tttacccgtg gcaatgcccg
      841 cgcagacgat ctggtacgca ataacggcta tgccgccaac gccatccag
//
LOCUS       DAZFDC010000403          878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFDC010000403 DAZFDC010000000
VERSION     DAZFDC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.406"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            157..726
                     /locus_tag="ACHJ48_005210"
     CDS             157..726
                     /locus_tag="ACHJ48_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO4710515.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            726..872
                     /locus_tag="ACHJ48_005211"
     CDS             726..872
                     /locus_tag="ACHJ48_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710516.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
BASE COUNT          236 a          185 c          229 g          228 t
ORIGIN      
        1 ccgtaatgat gcctttgtca tttctgcgca tctcacgcgc atctcacaac acagaacctt
       61 tcaggatgac ccttgaggat accggtttgg ctgtcggtgc ctttctgtgg gctggattcc
      121 tgtgagacaa ggttcatcac taaaaggaaa taaccgatga atatgatggc cgtgccgttt
      181 cacggcaact ctctttatgt agttaaccat aatggcgaac catacgttcc catgaaacct
      241 gtcgttgcgg ggatggggct ggcctggcaa tcacagttgg ctaagttaag acagcgtttt
      301 gcgtcaacta taacggaaat cgttatggtt gctgaggatg ggaaacaacg caatatggtg
      361 tccatgccac ttcgaaaact tgccggctgg ctacaaacca ttaatcccaa caaagtaaaa
      421 cccgaaatcc gcgataaggt catccggtat caggaagagt gcgacgatgt tctttacgag
      481 tactggacga agggttttgt cgttaatccc cgtaaaatga gcgtgatgga agaactcaac
      541 caggcttgtg ctgacatgaa acgggataaa aacattgcca gtgtgtttgc taccgggctg
      601 aatgagtgga aacaggttaa agccgcgcat gtatcaaaaa tccgtacgct ggtaaatgaa
      661 gcgaatatgc tgattgattt tgtcctggct gatacaggca aagggaaaat aacaaaggcg
      721 gattgatggg gtggctaatg atatcagata aactcataac gctggtgaag agcctctgtg
      781 tacttgtcgg catttcattt ttagtcatgc tggttgccat tttcttttcc accgcctggc
      841 gagtcctgac gttatcggga ctggtggggt gaaagaga
//
LOCUS       DAZFDC010000404          868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFDC010000404 DAZFDC010000000
VERSION     DAZFDC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.407"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(39..737)
                     /locus_tag="ACHJ48_005212"
     CDS             complement(39..737)
                     /locus_tag="ACHJ48_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4710517.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLREIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(737..>868)
                     /locus_tag="ACHJ48_005213"
     CDS             complement(737..>868)
                     /locus_tag="ACHJ48_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4710518.1"
                     /translation="GWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSAEFKQVVN"
BASE COUNT          192 a          302 c          195 g          179 t
ORIGIN      
        1 ccagaatcgc tgattcagtc tgtgtcataa accgggattt actgcgaaag tttattaatg
       61 gaaaggaaac cgccaaaatt agccaccatg ccgcgcatct cacacccgcg catgcacttg
      121 ctgcatctgt ccttacggat atcggtggtg ggtttatcga actcatccgc cacagccccg
      181 cccgtgtaac cacactcatc agagcggtag gtccacatac aggtattcgc cagcataatg
      241 cgaccgggaa acagcgctcc gtccgtctcc gtcggtgtgg ccagcacaaa cgaggctgtc
      301 atggccgtca gctctgacat ctgctccacc acccagcggt cgctcagctc ctgctccggg
      361 tccgcttccg gattgcccgc cacaaaattc accgcatcca gaaaacgggc atacacccgg
      421 cggcggacca ccgtggcccc caccaggctc tgcaggtcct ccgccatccc ggtgaccaga
      481 ccaaacagat tggacaccgt cagcgacggt ctggcactgc tgcccttccc gttcatctca
      541 aaaccgctgc cgtcaatcgg gtatacctga tattgccgcc cctgccaggt gaccgcctcc
      601 cctttttcat tcagctcatt acagaaaaaa taccgctcac cgccctgcac cgtcaggtca
      661 atttccctga gcaccacccg cggtgactgc tctgatttaa ccgactcgtt cagactttct
      721 tcgtgaatat cctgcatcag ttcaccacct gcttaaactc ggcactgaat tcaacccgca
      781 gcatgcgaac ccgtgatgac caggcggcac aggtcacctt tatctgccgc caggcataag
      841 gcggtgtcca cagaaacgcc ttccagcc
//
LOCUS       DAZFDC010000405          855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFDC010000405 DAZFDC010000000
VERSION     DAZFDC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.408"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..177
                     /locus_tag="ACHJ48_005214"
     CDS             <1..177
                     /locus_tag="ACHJ48_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4710519.1"
                     /translation="FPNQMIEQINIALDQKGSGNFSAWVIEACRRRLINEKHAQFVPN
                     KDKHDQSTCSDRFT"
     gene            complement(259..573)
                     /locus_tag="ACHJ48_005215"
     CDS             complement(259..573)
                     /locus_tag="ACHJ48_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO4710520.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          263 a          212 c          132 g          248 t
ORIGIN      
        1 tttcctaacc aaatgattga acaaattaac atcgctcttg atcagaaagg ttcaggtaat
       61 ttttcagcgt gggttattga agcctgcaga agaagattaa ttaatgaaaa acatgctcaa
      121 tttgtaccca acaaagacaa acacgaccag agcacctgtt cagacaggtt tacttaaacg
      181 acttatatat gacacaaaaa gcgaccacta aagtcgcttt ttcttatggt aacaggcaat
      241 aacgctctca gatatttttt agcatttttt tgaccgcgcg tttccggacg tattctgttc
      301 tcctgtccct ttatatcgtc ggaatacccg ccgctcttca aatcccattc ccaactcaga
      361 atgtagtctg ttgaccgctt gttttatttc ggtcaggttc accggtgaaa ccggagtccg
      421 gcgcgcctta cgcaaacact ctgctcgttt ctgtgccgcc acttttcttt tctggtcatc
      481 acttagctgt accatcactt ttgcccatcg ttcagctgct ctccggtaca gtcctttttt
      541 ctccagacat tctgccacgt gatcatgtag cataagtgac ctccgattat ctacagactg
      601 ccatcctgaa tttaccttcc cttaatgaaa taacaataaa aaacaaacca cgcaaaaaca
      661 ataaaacaac acacaaaaaa aactaaataa taaacaaaaa taatcacctt attttattat
      721 tttttgaggg agcaattact gaacaaaaaa cgctgactat atactcaaaa ccaaacaact
      781 attctgccaa tcaggtatca tggcaacaca cggaattacc gtgtttttgc cttctctgcc
      841 catacaatac gggca
//
LOCUS       DAZFDC010000406          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFDC010000406 DAZFDC010000000
VERSION     DAZFDC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.409"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            192..794
                     /gene="fimB"
                     /locus_tag="ACHJ48_005216"
     CDS             192..794
                     /gene="fimB"
                     /locus_tag="ACHJ48_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO4710521.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          250 a          160 c          186 g          258 t
ORIGIN      
        1 catagcgttt gtatggcaac gttattataa ttaactgttg ctactccatt taagttcact
       61 cagaagaact ggtccactta cgttagttat taagcaaacg ttcgctttta taaacataat
      121 caggataaaa atgttggatt attgctaacc tagcacagct agtgcgcgtc tgtaattata
      181 agggaaaaac gatgaagaat aaggctgata acaaaaaaag gaacttcctg acccatagtg
      241 aaatcgaatc actccttaaa gcagcaaata ccgggcctca tgcagcacgt aattattgtc
      301 tgactttgct ttgttttatt catggtttcc gggcgagtga aatttgtcga ttgaggattt
      361 cggatattga tcttaaggca aagtgtatat atatccatcg attaaaaaaa ggcttttcaa
      421 caacgcaccc gctattgaac aaagaagttc aggctttaaa aaactggttg agtatccgta
      481 cttcttatcc gcatgctgag agcgagtggg tatttttatc gcgtaagggg aatccgcttt
      541 ctcggcaaca attttaccat attatctcga cttccggtgg taatgccggg ttgtcactgg
      601 agattcatcc gcacatgtta cgccattcgt gtggttttgc tttggcgaat atgggaatag
      661 atacgcgact tatccaggat tatcttgggc atcgcaatat tcgtcatact gtctggtata
      721 ctgccagcaa tgcagggcgt ttttacggca tctgggatag agccagagga cgacagcgtc
      781 acgctgtttt atagagatag ttgagatacc agggatggtg ttaaaaacta cattattctt
      841 acgatatatc aaaa
//
LOCUS       DAZFDC010000407          852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFDC010000407 DAZFDC010000000
VERSION     DAZFDC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.410"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..819
                     /locus_tag="ACHJ48_005217"
     CDS             <1..819
                     /locus_tag="ACHJ48_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFO4710522.1"
                     /translation="DVSPALVSKVTDAVMEQVVEWQNRPLDAVYPIVYLDCIVLKVRQ
                     DSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTELKNRGLNDILIACVD
                     GLKGFPDAINTVYPEARIQLCIVHMVRNSLRFVSWKDYKAVTRDLKAIYQAPTEEAGQ
                     QALEAFASAWDSRYPQISRSWQANWTNLAMFFAYPADIRKVIYTTNAIESLNSVIRHA
                     IKKRKVFPTDDSVKKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT          214 a          227 c          239 g          172 t
ORIGIN      
        1 gatgtctcac cggcgctggt atcgaaggtc accgatgccg ttatggagca ggtagtcgaa
       61 tggcaaaacc ggccactgga tgcggtctat cccattgttt atcttgactg tattgtcctg
      121 aaggttcggc aggacagtcg cgtaatcaat aaatccgtat tcctcgcact gggtatcaat
      181 atcgaaggcc agaaagagtt actgggtatg tggctggccg aaaacgaggg agcgaagttc
      241 tggctcaatg tgctgactga actaaaaaac cgcggcctga acgatattct catagcctgt
      301 gtcgacgggc tgaaaggctt cccggacgct atcaacacgg tgtatccgga agcccgcatc
      361 cagctgtgca tcgtgcatat ggtgcgcaac agcctgcggt tcgtttcctg gaaggactac
      421 aaagccgtca cccgcgacct gaaagccatc taccaggccc ccacagaaga agcaggccag
      481 caggcgctgg aagcgttcgc cagcgcctgg gacagtcgct acccgcagat aagccggagc
      541 tggcaggcaa actggaccaa cctggcgatg ttcttcgctt acccggcaga tatccgcaaa
      601 gtcatctaca caaccaacgc catcgagtcg ctgaacagcg taatccggca tgccatcaaa
      661 aaacgcaagg tgttcccgac ggatgactca gtgaaaaagg tcgtgtggct ggcaatccag
      721 gcggcttcac agaaatggac gatgccactg cgggactggc gtatggcaat gagccgcttt
      781 attatcgagt tcggtgaccg cctggacggt cacttctgag aaaaggcatt tacacagaat
      841 cgtgtacagg gt
//
LOCUS       DAZFDC010000408          841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFDC010000408 DAZFDC010000000
VERSION     DAZFDC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.411"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>841
                     /locus_tag="ACHJ48_005218"
     CDS             <1..>841
                     /locus_tag="ACHJ48_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710523.1"
                     /translation="YRDKRIAKFDFIKFVSESDFQLSELDQLAQVESRQITSEIRGGE
                     FVDDDFRQLVEMLKLNMICTWDLRSSVYYLTMKMLGQPGTPEYAKYSELSASIFNELS
                     DASDTKGYWSTDVKLVSSSGHEYTEREFELEREKSTRGKGGMTRSLEMFIASLNWLAY
                     KSIYYSVIAKHFKADSFIHPIRHAYQLHWMKKTGAFGHDYTARLVQSLSDKISTARSE
                     IVDHGRTSTISLDLPIFSAWLANETGNVSQVINSALELRTHDHFRTCRDVIREIHVTY
                     DESG"
BASE COUNT          265 a          134 c          178 g          264 t
ORIGIN      
        1 aatatagaga taaacgcatt gctaaatttg actttattaa atttgtatct gaaagtgatt
       61 ttcaactcag tgaacttgat cagttagcac aagttgaatc tcggcaaatt acttccgaaa
      121 ttagaggtgg ggagtttgtt gacgatgatt ttaggcaact tgtagagatg cttaagctga
      181 atatgatttg cacatgggat cttcggtcta gcgtgtatta cttaacaatg aaaatgcttg
      241 gtcagcccgg tactcctgaa tatgcgaaat atagtgaact aagtgcatcg atttttaatg
      301 aattatcaga tgcttctgat acgaaagggt actggtcaac cgatgtgaag ctagtgagtt
      361 catctggaca tgaatatact gagcgtgaat ttgaactaga aagggaaaaa tctactcgcg
      421 gtaaaggagg tatgactcgt tcacttgaaa tgtttatcgc atcgctaaat tggttggcat
      481 ataaatctat ttattacagc gtcattgcta agcattttaa agcagactca tttattcatc
      541 caataaggca tgcatatcaa ttgcattgga tgaaaaaaac aggtgctttt ggtcatgatt
      601 atactgcaag gttggtgcaa tcgttgtctg ataaaatcag tactgcaaga tctgaaattg
      661 tagatcatgg acgcacatct actatttcat tagatttacc aatattttct gcatggctag
      721 caaacgaaac aggaaatgtc tctcaagtta tcaattcagc tttagagctt agaacacatg
      781 atcattttcg tacgtgtcga gatgtaatcc gagagattca tgtgacctac gatgaatcgg
      841 g
//
LOCUS       DAZFDC010000409          840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFDC010000409 DAZFDC010000000
VERSION     DAZFDC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.412"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            115..762
                     /gene="nleG"
                     /locus_tag="ACHJ48_005219"
     CDS             115..762
                     /gene="nleG"
                     /locus_tag="ACHJ48_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO4710524.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
BASE COUNT          258 a          169 c          153 g          260 t
ORIGIN      
        1 tgtaaatagc attcgttcaa aatttaattt tttgtttgaa tcctatcaca tatttagtat
       61 cttgactcct gtcaccaata tggttcaata acatagacta aagagggaga aaatatgcct
      121 gtcatattaa acttttcgag tgaaagagta ttgtctgaaa gtgaactgga ggctttacgt
      181 catgtcggaa gagttagtca gagtgaacag cttgttgtga gaggccgcac tatgagactt
      241 catcatattt catttatgga cagttttagc gtggaaccgg tttccggcgg actgttggat
      301 cgccttagtg caagagggca tcgccttctc gcagaaaatc tggagataca gcttaacaga
      361 ggccatactt ttttgcaggc attccgttta tatatggaac agagccgtgc aaccccatgt
      421 acgcgccaaa acgtcagtag tgcaatacaa aacaaaatta actctcatgc atttactgtt
      481 agtcatcaag acttttcctg tcatgaacaa cacctgaatt gcccgataac attatgcatt
      541 cctgaaactg gtgtttttgt cagaaatgca aaaaactcag aaatatgttc attatatgac
      601 cacaatgcac tcactgaact tatccgacgc aacgctccac accctctcag ccgtgaaccc
      661 tttgttccgg aaatgattgt cagcaaagat gaatgccatt ttaatctaat agaacaatat
      721 ttttgcatat tagccacaca aaatatatgc actagaattt gactgtatgg ttcgatttaa
      781 atgttgcaaa attaatttta ctagttattg ccatctattt catactccct aattcttgat
//
LOCUS       DAZFDC010000410          838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFDC010000410 DAZFDC010000000
VERSION     DAZFDC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.413"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..563)
                     /gene="fimE"
                     /locus_tag="ACHJ48_005220"
     CDS             complement(<1..563)
                     /gene="fimE"
                     /locus_tag="ACHJ48_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO4710525.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNL"
BASE COUNT          273 a          189 c          164 g          212 t
ORIGIN      
        1 agattatttc tttcccataa tccggcaaaa cgagcagcat tactggcggt ataacgtaca
       61 gtatggcgaa tatttcgatg cccgagataa tcctgaatta aacgagtatc tgcaccacgc
      121 tccgccagtt cataaccgca agcatgcctt aacatatgag gatgagtctg cgttacggtt
      181 ccagcttcaa taccggcatc acgaataatg cgataggcct gctggcgaga aagccgactc
      241 ccgcggcgag aaataaatat ggcgtcggtc cggtcagcgc ctttccagtt agcacgttct
      301 agggtccagc gttccacagc ttcacgctca tcaaaacgta acgggtgaac ggtagaaaat
      361 ccgttcttca gtcggcgaat atttattcgg ccttcattaa ggtcaagatc ctgataatgc
      421 agatcaagca gttcactaat acgcatccca tgccgatatg ccaacagaat aagacaataa
      481 tctctggctc ccgttgcccc gtaacaaacc gcctgcatca tggcctgaac ttctttaccg
      541 gtaagataac gacgtttact cacaatagta gtactcctaa ctgagatata tttgaatgaa
      601 tacctatagg aaacctcaat cggtcaaaat tagcccaata gataaaaaga taaccacaca
      661 cgaagtgatg tggctatcag tcaattacat taaatatcat acaaaaataa aatatcactg
      721 atgtgataac atctattttc attgatttga ttcaatataa caatagattg cagtggatta
      781 acttccagtt gcttaaaaaa taaaggaaaa agtgcagaaa caggagagtt taagtgat
//
LOCUS       DAZFDC010000411          837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFDC010000411 DAZFDC010000000
VERSION     DAZFDC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.414"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(181..792)
                     /gene="map"
                     /locus_tag="ACHJ48_005221"
     CDS             complement(181..792)
                     /gene="map"
                     /locus_tag="ACHJ48_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector Map (Rho
                     guanine exchange factor)"
                     /protein_id="HFO4710526.1"
                     /translation="MFSPMTMAGRSLVQATAQTLKPAVTRAAMQAGTGATGMRFMPVQ
                     SNFVINHGKLTNQLLQAVAKQTRNGDTQQWFQQEQTTYISRTVNRTLDDYCRSNNSVI
                     SKETKGHIFRAVENALQQPLDMNGAQSSIGHFLQSNKYFNQKVDEQCGKRVDPITRFN
                     TQTKMIEQVSQEIFERNFSGFKVSEIKAITQNAILEHVQDTRL"
BASE COUNT          239 a          181 c          145 g          272 t
ORIGIN      
        1 ataaaattgc agcaaacatt catttatatt aaaaaacaca gccaaagaaa accaattaag
       61 caaactataa atcttaatta ataataaagt ttattaagtt tctttatcgc gttttttctt
      121 acatcaacaa aggccatgct atacaccagg aagcttaatt tatacctgac aaggttatcc
      181 ctacaatcgg gtatcctgta catgctctaa aatcgcattc tgagtaatcg cttttatctc
      241 actgactttg aagccgctaa agttgcgttc aaatatttct tgtgagactt gttctatcat
      301 cttcgtctgg gtgttaaaac gtgttattgg atccactctt ttgccgcact gctcatcaac
      361 tttctgatta aagtatttat tggactgcaa gaagtgacca attgatgatt gtgccccgtt
      421 catatcaaga ggttgctgta acgcattttc aacagctcta aaaatgtgac ctttagtctc
      481 cttactaatg accgaattat tactcctgca atagtcatcc agagttctat ttactgttct
      541 ggatatataa gtcgtctgct cctgctggaa ccattgttgg gtatcgccat tacgagtttg
      601 tttggctaca gcctgcagga gttggttagt cagtttgcca tgattaatca caaagttcga
      661 ctgcacgggc atgaacctca tacccgtggc tcccgtaccc gcttgcattg cagctctggt
      721 aaccgcaggt ttaagcgtct gtgcagtcgc ctgaactaac gatctgcctg ccattgtcat
      781 tggactaaac ataccataaa cctttataaa ttatatttcg acagtaaaaa aattgct
//
LOCUS       DAZFDC010000412          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFDC010000412 DAZFDC010000000
VERSION     DAZFDC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.415"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            219..294
                     /locus_tag="ACHJ48_005222"
     tRNA            219..294
                     /locus_tag="ACHJ48_005222"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:252..254,aa:Ile,seq:cat)
BASE COUNT          244 a          153 c          187 g          252 t
ORIGIN      
        1 aagtatcatt gaatcgatga taactataga ttgtgctaat tattaacatt tgcttgttga
       61 ctgcgagtat cgactttgcg gaatggaggt ggtgtcgggt tttatgtcgc atgtattgac
      121 gacccaggat ggatgagaac gcataaaaat ctgtgattta catcataaga tcatcatgtt
      181 tattgcgtgg gttataagta ttgttattgc atacttaagg ccctttagct cagtggttag
      241 agcaggcgac tcataatcgt ccggtcgctg gttcaagtcc agcaagggcc accaaatcat
      301 cattcatgta tcaggaagag ttaagggtac aagggttact cgatggcggt ctatatttca
      361 cgattaacgc catttcaagg aagaagtgcg gtgggtggtt tcgaatgagg gctggtgttt
      421 tggtacgtgg cacgccactg caatgtattt atctatcagt actcattcaa aagtatttgg
      481 aaaaaatgcc tgctatgcca agcgggcaaa ttgcttacat gtggaattaa gtaattaacg
      541 ataaagccag cgtattatca tttattatac ccattcattt aatcaggtta attactcaaa
      601 tcactgaact attctctgtt cgctcactga tgataaggga gtaccacaac caatgtatgt
      661 agaacaatgc cgtagagtaa ggatgaaatg tctctgccac gccttaaaca tctactactg
      721 actagaacac ccgccgcagg tagatgagtt ttgtgagcgt tacaggagcg tggctatgct
      781 cattaatcaa cgctatttag atataaattt ctaacactcg aaaaacgatt ttcact
//
LOCUS       DAZFDC010000413          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFDC010000413 DAZFDC010000000
VERSION     DAZFDC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.416"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            271..>836
                     /gene="tccP"
                     /locus_tag="ACHJ48_005223"
                     /gene_synonym="espF(U)"
     CDS             271..>836
                     /gene="tccP"
                     /locus_tag="ACHJ48_005223"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
                     /protein_id="HFO4710527.1"
                     /translation="MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFS
                     PSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSM
                     AEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLVQHLAEHGIQPARNMAEHIPPAPNWP
                     APPLPVQNEQSRPLPDVAQRLVQHLAEHG"
BASE COUNT          248 a          211 c          157 g          220 t
ORIGIN      
        1 cgcttaagag tagatttttg gcattttggg aagatcagcg ggacataaga gacatacaac
       61 acaggaggaa tggctgtttt ttaatagtgc tttgaattcc gcatgaaacc tttcttagca
      121 gtcgaaaagc aaaacatacg ttttcttttt aacaataatt gatgagtatt gcatcgagtg
      181 tcatttatac atcgccgcat ttatttgcca ctataaaaag gctgattaca tatctatcgg
      241 agaactaaaa ttacttataa ggtaatctgc atgattaaca atgtttcttc actttttcca
      301 accgtcaacc gcaatattac agctgtatat aaaaaaagca gcttctctgt atcaccacag
      361 aaaatcacat taaatcctgt aaaaatcagc tcaccttttt caccaagcag tagctccatc
      421 agcgcaacaa ctctctttcg agccccaaac gcccattcgg catcatttca tcgacagtct
      481 actgctgaaa gttcgttaca tcaacaactt cctaatgtga ggcagcgcct gatacaacat
      541 cttgcagagc atggcattaa acctgcccgg agtatggctg aacatattcc tccggcacct
      601 aactggcctg cgccaccacc gccagtacaa aatgaacaat caagacctct gcctgatgtg
      661 gctcagcgtc tggtgcagca tcttgcagag catggcattc aaccagcccg gaatatggct
      721 gaacatattc ctccggcacc taactggcct gcgccaccac tgccagtaca aaatgaacaa
      781 tcaagacctc tgcctgatgt ggctcagcgt ctggtgcagc atcttgcaga gcatgg
//
LOCUS       DAZFDC010000414          824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFDC010000414 DAZFDC010000000
VERSION     DAZFDC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.417"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>824
                     /locus_tag="ACHJ48_005224"
     CDS             <1..>824
                     /locus_tag="ACHJ48_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4710528.1"
                     /translation="PSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQ
                     NGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTGLLETQTVDFT
                     LGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAASRTLTLDREVTLPETGTPTVNL
                     INGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTD
                     GTFAITAVQHVPEKEAIVDNGASFEPLSGSLNSVIPPAVQHLTVEVSAADGQYLAQA"
BASE COUNT          165 a          244 c          271 g          144 t
ORIGIN      
        1 ccgtcggatg tggtgtggcc gtacaccaac agtgatgtgg tggtggatga taacggcgtg
       61 gggtttcgct acagcttcag cgccctgaag gaccgccaca cggcggtgga ggtgaattac
      121 accgacccgc agaacggctg gcagacctcc acggaactgg tggaagaccc ggaagccata
      181 ctgcgctacg ggcgcaacct gctgaagatg gatgcgttcg gctgcaccag tcgcggtcag
      241 gcccaccgtg ccgggctgtg ggtgataaag accggactgc tggaaacgca gacggtggat
      301 ttcacgctcg ggtctcaggg gctgcgtcac acacccggtg acattattga aatctgtgat
      361 aacgactatg ccgggaccat gaccggcgga cgtgtcctgt ccatcgatgc cgccagccgc
      421 accctgacac tggaccgtga ggtgaccctg ccggagacag gtacaccgac ggtgaacctg
      481 attaacggca gcggtaagcc ggtgagcgtg gccatcactg cacaccccgc gccggaccgg
      541 atacaggtca gcaccctgcc ggatggcgtg gagacatacg gtgtgtgggg actctccctg
      601 ccgtcactgc gtcgtcgcct gttccgctgt gtctccatcc gggaaaacac ggacggcacc
      661 tttgccatca cggcggtgca gcacgtaccg gaaaaagaag ccattgtgga taacggggcc
      721 agctttgagc cactgtccgg ttcgctgaac agcgtcatcc cgccggctgt gcagcacctg
      781 acggtggagg tgagcgcggc tgacggtcag tatctggcac aggc
//
LOCUS       DAZFDC010000415          816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFDC010000415 DAZFDC010000000
VERSION     DAZFDC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.418"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..197)
                     /locus_tag="ACHJ48_005225"
     CDS             complement(<1..197)
                     /locus_tag="ACHJ48_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710529.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTV"
     gene            complement(261..>816)
                     /locus_tag="ACHJ48_005226"
     CDS             complement(261..>816)
                     /locus_tag="ACHJ48_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO4710530.1"
                     /translation="GRDRETREWLGWGHAWAHETAVVRRKSEASRFQDLVACGDMTIV
                     RRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWK
                     LGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMA
                     LFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT          179 a          272 c          198 g          167 t
ORIGIN      
        1 accgtacact gccccaccgg gcgggaatgg tcatgctgcc agagaaacgg gatcgcactg
       61 ccaaactccg cgccctccgg ctccaggatg tcaccatccc gatccggaga aggcgttgac
      121 gcaatcccgg tgatcacccg ttcatcctca ctgaaggatt tcaccgtcag cagggaacag
      181 gcccgtttaa gagtcacatc agcctcctga aaataaaaaa accgccgcag cggttcatga
      241 tggttacagt gtgaacaggg ttatatgaaa aaaaccgcat attctttctt tttcggttcc
      301 gggttaaggg acatcaggga gaccgcattg aacagcgcca tcagcgggtc aatttttccc
      361 cgtccactgg cctgtttggt gataagaatg gcgttacctt taggctccac ccgggcattg
      421 ccgacacacc aggccatcag gggctggtca ccatgcacca gcaccccttc agccagtttg
      481 cgctcggtgg ttttaatggc cccgcccagt ttccagccct ggcttatccc caccacaatt
      541 ccgtcgggga tcccggcttc cgccagtgaa tccagaatct gccccacccc tgacgggtca
      601 ataccgatat ggtccagtaa ctcagcctca tgaatgcgac gcacatattc cgccacttcc
      661 gccgtgtcat ccccgacacg ccggacaatg gtcatatctc cacaggcaac aagatcctga
      721 aaccgggacg cctcgctctt ccgtcggacc accgcggttt catgcgccca ggcatggccc
      781 cagcccagcc attcgcgggt ttccctgtca cggcca
//
LOCUS       DAZFDC010000416          813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFDC010000416 DAZFDC010000000
VERSION     DAZFDC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.419"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..723
                     /gene="ydeO"
                     /locus_tag="ACHJ48_005227"
     CDS             <1..723
                     /gene="ydeO"
                     /locus_tag="ACHJ48_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO4710531.1"
                     /translation="FNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENT
                     IKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCL
                     SIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQ
                     EQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVS
                     KEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          252 a          162 c          163 g          236 t
ORIGIN      
        1 ttcaacgcta acattttaga taaagattac gccttctctg acggcgagat cctgatggta
       61 gataacgctg ttcgtacgca ttttgaacct tatgagcggc attttaaaga aatcggattt
      121 aatgaaaata ccattaaaaa atatctacaa tgcactaaca tccagacagt gacgatgcct
      181 gttcctgcga agtttttacg tgcttcgaat gtaccgactg gattgcttaa tgaaatgatt
      241 gcttatctca actcggaaga acgcaatcat cataattttt cagaactttt gcttttttct
      301 tgcctgtcta tttttgccac atgcaaaggt ttcattacac tattaactaa cggtgtgcta
      361 tccgtttcgg ggaaagtgag aaatattgtc aacatgaagc tggcgcaccc atggaagctg
      421 aaagatattt gtgactgcct gtacatcagt gaaagcctgt tgaagaaaaa actaaagcaa
      481 gagcaaacga cattctcaca gattctttta gatgccagaa tgcagcacgc aaaaaatttg
      541 atacgcgtag aaggttcagt caataaaatt gccgaacaat gtggttatgc cagtacatct
      601 tattttattt atgcgttccg caaacatttc ggcaacagtc caaagagagt ttctaaggag
      661 taccgttgtc aaaggcacac gggtatgaat acgggcaaca cgatgaatgc tttagctatt
      721 tgattatttg ctaacgagta gtcaaccacg caccctacgt aaaaattaaa aatggggcag
      781 tcattccctg ccccgcgttg tttttaggcg ata
//
LOCUS       DAZFDC010000417          812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFDC010000417 DAZFDC010000000
VERSION     DAZFDC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.420"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..246
                     /locus_tag="ACHJ48_005228"
     CDS             <1..246
                     /locus_tag="ACHJ48_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710532.1"
                     /translation="NEKMLNLNDNDEKVKIENDIKKIDKKIEEIDNAIRDLSSETERF
                     KIKYQQENTLNDVVQNIRTWLKENQNMVKAIKKIDTE"
BASE COUNT          301 a          129 c          150 g          232 t
ORIGIN      
        1 aatgagaaga tgcttaattt aaatgataat gatgaaaaag tcaaaatcga aaatgatata
       61 aagaaaatag ataaaaaaat tgaagaaata gataatgcaa tccgggatct ttcctctgaa
      121 acagagaggt tcaagataaa gtatcaacaa gagaatacac ttaatgatgt agttcaaaat
      181 atcaggactt ggctaaaaga aaatcaaaat atggtgaaag ctattaagaa aattgatact
      241 gaataacatg gcgaataaat aaacggatca agtcgttcct atgacaatga ggttatacga
      301 aaagacgctt actcatcaga aatctggtat gctttaatta aaccagtgtc gctgatgtgt
      361 aagcgttgac gttgggagac gattaaatgg ttataccact cctacgtctt ttccatacgg
      421 cgtataagag tgcaacatgt tgagttttcc gagtgaatgg tgcattttac taaccgagcc
      481 atttgaatca accaacgcaa taaaaaagac tggaataata agcctgtatt tattatttat
      541 cgcaatcgca ctttacggtt ttatccaact gaaaagagac tgcttccagc ggttgtggat
      601 tatccctggg cgatagtcat agtctggtgt caggattcac aaatgacctg tttccttaca
      661 tggatataaa cacaacatag agtgcggtag ttaatctgaa taatctaatt tcggctattc
      721 agcacaaaac aacattattg attctttata agagagaatc tctccatcat ttttatataa
      781 atcattatta agaaaatcca gaagctcttg cc
//
LOCUS       DAZFDC010000418          809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFDC010000418 DAZFDC010000000
VERSION     DAZFDC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.421"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..156
                     /locus_tag="ACHJ48_005229"
     CDS             61..156
                     /locus_tag="ACHJ48_005229"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO4710533.1"
                     /translation="MEGKQPKNSGGNFTMLIAKKDGQVLNVIHTK"
BASE COUNT          278 a          139 c          170 g          222 t
ORIGIN      
        1 aaagaaaaag cagaatttca gaaaccatat agtattacag atgacaataa ttgttggaaa
       61 atcgagggaa aacaaccgaa gaattcagga ggaaatttta caatgctaat agcgaaaaaa
      121 gatgggcaag tgttgaatgt aatacacaca aaataatcca ctgtacagtc gattcaaata
      181 ttttaccaag gtgtgtttcg taacaatttt gtgtacaata tgactctaca tatcgacgaa
      241 aaaaccactt gatttgacag gaatacattc aaaaaatttc ttttttcatt tcaggagcga
      301 ttttcatctg ttacttgaag tatctgtgag attaagtgac tccccctaac gtcgtactaa
      361 atagtaatta ctggccatac cgggagcagt attcggcgct ccccacatgc agcagaataa
      421 ccgcatcagc gaagatgtat ttttactatc acaatgtgta cggacggttt atggaaaaga
      481 atgaacatta gatacgcgat gcggcggcag gttacacggt tatcactacg atagccaata
      541 ctggctggtg cattaccgcc gtgaacaata ggacgccgta ctgctggaaa tttcttacct
      601 ttataactcc ccgcgccgcc gtatcgggaa acgggaagca atcaagatga ggctgatata
      661 atgtgcggaa tgcctgagta tttttagaat aggggggtaa tatgtttgat agaatatatc
      721 ttggtgatcg agcaataaaa aaattgaatt tgatttatgg agtaaacaaa ttagaattca
      781 ggttaattta atctcaagga tagcctatg
//
LOCUS       DAZFDC010000419          807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFDC010000419 DAZFDC010000000
VERSION     DAZFDC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.422"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..655)
                     /locus_tag="ACHJ48_005230"
     CDS             complement(<1..655)
                     /locus_tag="ACHJ48_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4710534.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNAEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     "
     gene            complement(655..>807)
                     /locus_tag="ACHJ48_005231"
     CDS             complement(655..>807)
                     /locus_tag="ACHJ48_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4710535.1"
                     /translation="EFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYSDFSCT
                     IEQVVN"
BASE COUNT          172 a          296 c          182 g          157 t
ORIGIN      
        1 ccgccatgcc gcgcatctca cacccgcgca tgcacttgct gcatctgtcc ttacggatat
       61 ccgtggtggg gttgtcgaac tcatccgcca cagccccgcc cgtgtaacca cactcatcag
      121 agcggtaggt ccacatacag gtattcgcca gcatgatgcg accgggaaac agcgccccat
      181 ccgtctcggt cggtgtagcc agcacaaacg aggccgtcat ggctgtcagc tgcgacatct
      241 gctccaccac ccagcggtca ctcagctcct gctccgggtc cgcctccgca ttgcccgcaa
      301 cgaaattcac cgcatccaga aaacgggcat acacccggcg gcggaccacc gtggccccca
      361 ccagactctg caggtcctcc gccatcccgg tgacaaggcc aaacagattg gacaccgtca
      421 gcgacgggcg ggcactgctg ccccggccgt tcatctcaaa gccgctgccg tcaatcgggt
      481 atgcctcata cttacgcccc tgccaggtga ccggctcccc tttttcattc agctcattac
      541 agaaaaaata ccgctcacca ccctgtaccg tcaggtcgat ttcccagagt accacccgcg
      601 gtgactgctc tgacttaacc gactcgttca gactttcttc gcgaatatcc tgcatcagtt
      661 caccacctgc tcaatcgtac aactgaaatc actgtacctg gcgttatctg tgacgctcca
      721 ctcccggcac accaccctca ccgtccggtt atgtttcggc ggtcgccaca aaaaggcacg
      781 gtaaccacca tgccacgata aaaactc
//
LOCUS       DAZFDC010000420          800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFDC010000420 DAZFDC010000000
VERSION     DAZFDC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.423"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            60..692
                     /locus_tag="ACHJ48_005232"
     CDS             60..692
                     /locus_tag="ACHJ48_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO4710536.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGAELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
     gene            complement(711..>800)
                     /locus_tag="ACHJ48_005233"
     CDS             complement(711..>800)
                     /locus_tag="ACHJ48_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO4710537.1"
                     /translation="QKKRLQYAKKIYIVYTRLNELDNRKALAK"
BASE COUNT          273 a          116 c          167 g          244 t
ORIGIN      
        1 ataatgaaag ggatgattat aaatgaatgc gttaaagcat ttacactcag gatgaatata
       61 tgggaaaaat taaaattgta gtttcagatc agcagccgtt tatgattgat gggataattg
      121 gattcctcgg acattatccc gatttatatg aggttgttgg gggctataaa gatctgaaga
      181 aagctatagc ggagtgtaac aaatctgcag cccaaatatt tattttagga gaatttgcag
      241 gtggaatgat gggggccgaa ctcgtaaaat gggttaaatc gcataaaata gatgctcata
      301 ttattacatt tgtagcaaaa atgccgtata ttgattcaat aaaattgctt gaagcaggtg
      361 cgaaaggatg cgtatggaaa accagtcacc cggcgaaact taatcgcgct attgattcga
      421 ttagtaacgg ctacacttat tttgatagtg tacatatgga ttgtgagaaa atatcttcca
      481 gatattcctc tgataatcaa cttacaaatc gtgagtctga aatattacaa ctcatagctg
      541 atggaaaaac aaacaaagaa atcgctaatt tcttgcagtt aagcaggaaa acagtcgaaa
      601 ctcatagact caatatcatg aagaaattag atgttcacag tggtatcgag ttgatcaaaa
      661 cagctttacg tatgggtgtg tgcactatat aaaaaaaaga ggctttaggc tcattttgct
      721 aaagccttac gattgtctag ttcattaagc ctggtatata caatatatat ttttttggca
      781 tattgcagtc gctttttttg
//
LOCUS       DAZFDC010000421          800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFDC010000421 DAZFDC010000000
VERSION     DAZFDC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.424"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>800
                     /locus_tag="ACHJ48_005234"
     CDS             <1..>800
                     /locus_tag="ACHJ48_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFO4710538.1"
                     /translation="REQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLG"
BASE COUNT          193 a          186 c          251 g          170 t
ORIGIN      
        1 tgcgtgagca gtacggggtc ggaccggtat gcagcgaact gcatattgcc ccgtcaacgt
       61 attaccattg tcagcaacag cgacatcatc cggataaacg cagtgcccgt gcgcagcacg
      121 acgactggct gaagagagag atacagcgcg tatacgatga aaatcatcag gtgtacggtg
      181 tgcgtaaagt ctggcgtcag ttgttacggg aaggaatcag ggtggccaga tgtacagtgg
      241 cacgtctcat ggcggttatg ggacttgccg gtgttctccg gggtaaaaag gtccgtacga
      301 ccatcagccg gaaagccgtt gccgcaggcg accgcgtaaa ccgtcagttc gtggcagaac
      361 gacctgacca gctgtgggtg gctgatttta cttacgtcag cacatggcag ggcttcgtct
      421 atgtggcgtt tatcattgat gtgtttgccg gatacatcgt ggggtggcgg gtctcatcgt
      481 ctatggaaac gacattcgtg ctggatgcgc tggagcaggc gttgtgggcc cgtcgtccgt
      541 ctggcaccat ccatcacagc gataaaggct ctcagtatgt gtcactggcc tatacggagc
      601 gactaaaaga agccggatta ctggcatcaa cagggagtac aggcgactcg tatgacaacg
      661 cgatggctga gagcatcaat ggtctttaca aagcggaggt aatacaccgt aagagctgga
      721 aaaaccgtgc agaagtggaa ctggccacac taacgtgggt ggactggtat aacaatcgac
      781 gattgctggg aaggctgggc
//
LOCUS       DAZFDC010000422          798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFDC010000422 DAZFDC010000000
VERSION     DAZFDC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.425"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..471)
                     /locus_tag="ACHJ48_005235"
     CDS             complement(<1..471)
                     /locus_tag="ACHJ48_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO4710539.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEGL"
     gene            complement(585..>798)
                     /locus_tag="ACHJ48_005236"
     CDS             complement(585..>798)
                     /locus_tag="ACHJ48_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4710540.1"
                     /translation="REHGELLTAQLRLGPADILESDENGIIPEQDRVITQVVILDADK
                     KQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          232 a          224 c          141 g          201 t
ORIGIN      
        1 taagccttca ccaaccaaac gagaaaatgc agcggcatca aataaagtac atacatctga
       61 accatctgaa ttcttcacaa aaataccttt ttcaggctgc tccagtgtaa ttggacactg
      121 aatagcctca agcgggcact gaagtctctc tggacaaaca gaaaacttac attgagatat
      181 tttgttctga atactctctt gcgattgtgc ttcacctgag tccgaaagca tacttagcat
      241 tctctcacca agggctcgag gaccactatt aaatcccaaa agcaataact cagaaagcaa
      301 tccgccatta ccgccactgg atgaaaagcg cccattagta gaatcataca caatactgac
      361 agtttcacca ccgactgtaa tatctccgcg cccattggct acaattcggg cacgaacaac
      421 ctccacccca agattaaatg aatgtgatct aatatctgag gttaatggca taacaaagct
      481 ccctatttta atttgaactc cagacttaaa tagctgtaac aaacatcccc catacgtgac
      541 aacaaaaaac cggagccgga ctccggtttt tgtgaagctg tcggctattt catcccgcca
      601 atattttccc acgtcccgtc agcacgcagg atttgcagcg gtcttaccac acactgtatc
      661 tgctttttat ccgcatccag tatcaccacc tgcgtgatta ccctgtcctg ctccgggata
      721 ataccattct catctgactc caggatgtct gccggcccca gtcgcagctg tgctgtaagt
      781 aactccccgt gttcacgg
//
LOCUS       DAZFDC010000423          790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFDC010000423 DAZFDC010000000
VERSION     DAZFDC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.426"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            64..336
                     /locus_tag="ACHJ48_005237"
     CDS             64..336
                     /locus_tag="ACHJ48_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO4710541.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            413..628
                     /locus_tag="ACHJ48_005238"
     CDS             413..628
                     /locus_tag="ACHJ48_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO4710542.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            633..>790
                     /locus_tag="ACHJ48_005239"
     CDS             633..>790
                     /locus_tag="ACHJ48_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO4710543.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICR"
BASE COUNT          191 a          196 c          233 g          170 t
ORIGIN      
        1 aacccgaaga gccgctcatt tagcggcaac tttcttaatc acacctttcg acgagaaaat
       61 cccatgtcag aaattacatc cctggtcact gctgaagcag tgaaggaagt cctgcgctct
      121 gaagaagtcc tgagcgcact gaaacaaaaa cttcgccata acctggaagc gcgtcttgat
      181 gcagaggttg atgccattct ggatgagctg cttggtgtac aggcagagcc accgactgaa
      241 gcgggagata ccaccgcaga gagcggtgaa gttcagcctg aatcaccggt cgccgatgcg
      301 actgaacctc aacccgaatc ggtcatgatg ctgtaacggg gagtcagggc catcagtaaa
      361 cagctgctgg cctttttcat gttgtgagct tccggattgc gggagacggg gtatgtacca
      421 gatggaaaaa atcacaacag gtgtgtcata caccacgtca gcggtgggaa cgggctactg
      481 gttcctgcag ttgctggaca gggtttcccc gtctcagtgg gcggcaatag gcgtgctggg
      541 gagtctgctg tttggtctgc tgacgtacct gacgaacctg tatttcaaaa tcagagagga
      601 caggcgtaag gcggcacggg gagagtaagc tgatgagcag gaaactccgc tatggtttat
      661 cggctgccgt tctggcgctg attgccgcag gtgcttctgc gcctgaaatc ctcgaccagt
      721 ttctggatga aaaggaaggc aaccacacca cggcataccg tgatggtgcg ggtatctgga
      781 ccatctgccg
//
LOCUS       DAZFDC010000424          786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFDC010000424 DAZFDC010000000
VERSION     DAZFDC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.427"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..735
                     /gene="yeeN"
                     /locus_tag="ACHJ48_005240"
     CDS             19..735
                     /gene="yeeN"
                     /locus_tag="ACHJ48_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO4710544.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGISEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          257 a          138 c          167 g          224 t
ORIGIN      
        1 ttcgaggaga tcattcctgt gggacgtaaa tgggccaata ttgttgctaa aaaaacggct
       61 aaagacggtg caacgtctaa aatttatgca aaattcggtg tagaaatcta tgctgccgct
      121 aaacaaggtg aacccgatcc agaattaaac acatctttaa aattcgttat tgaacgtgca
      181 aagcaggcac aagttccaaa gcacgttatt gataaagcaa ttgataaagc caaaggcggc
      241 ggagatgaaa cattcgtgca gggccgttat gaaggctttg gtcctaatgg ctcaatgatt
      301 atcgccgaga cattgacttc aaatgttaac cgtacgattg ctaacgttcg cacaattttc
      361 aataaaaaag gcggcaatat cggagcggca ggttctgtca gctatatgtt tgacaatact
      421 ggtgtgattg tatttaaagg atcagaccct gaccatattt ttgaaatttt acttgaagct
      481 gaagttgatg ttcgtgatgt gactgaagaa gaaggtaaca ttgttattta taccgaacct
      541 actgaccttc ataaaggaat cgcggctcta aaagcagctg gaatcagtga gttctcaaca
      601 acagaattag aaatgattgc tcaatctgaa gttgagcttt caccagaaga tttagaaatc
      661 tttgaagggc ttgttgatgc ccttgaagat gacgacgatg tacaaaaagt ttatcataac
      721 gtcgcaaatc tctaattata ttttaaagaa atctgtcttt acggcagatt tctttaatct
      781 catata
//
LOCUS       DAZFDC010000425          785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFDC010000425 DAZFDC010000000
VERSION     DAZFDC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.428"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(317..739)
                     /locus_tag="ACHJ48_005241"
     CDS             complement(317..739)
                     /locus_tag="ACHJ48_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710545.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDMAY
                     LSRCLASIHPTLAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
BASE COUNT          243 a          111 c          158 g          273 t
ORIGIN      
        1 attaacttga tttaatgtat tgagataatt gtgatctact tctaatttgc atttttgtga
       61 cgtggtttta gccatgagta aatgcatttc ataagattta agtcgaattt cactgcaatt
      121 catctctgct taatgtaaaa ataatgttga aaataacaaa tggttaatga ttcatgcgaa
      181 ttatgacctt agttagctct tttaattttt cgtaaaaaag agaggtacat gaaggtgcta
      241 tcagtatgag taacgttaat caaaggtttt attacgtctg gcaataacgc aggccatttt
      301 tcgcgacagg gtggatttaa tcagtataat cctcatacaa tagttcacga ttaactgaac
      361 cgttaacggg gatttggtgt tgaaatttag aaatggcgga gtcaattaat gaagacaata
      421 tcgaaaaaat gactgtgtca gaattaattt caattaatcc ttctcgttgt tgaccaaaaa
      481 agatatttat cttgatttca tttgtactac ggcaatcaat atgtagtaat ttttgatcgc
      541 tgagattttc tgtttcataa atgtttttgg cgagagtcgg gtgtattgat gccagacatc
      601 tgctcagata agccatatcc tttagggaac accccgctga gtagaggatg tcagagaatt
      661 tttgtgtgtc aactgttgtc tctgaagcgt atgttgcatt gactggaagg gtattgaaag
      721 agatctgaga tggtatcatt atttaaatct ttccgtgagt aataaaaatt aatccccagg
      781 cattg
//
LOCUS       DAZFDC010000426          784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFDC010000426 DAZFDC010000000
VERSION     DAZFDC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.429"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            85..510
                     /locus_tag="ACHJ48_005242"
     CDS             85..510
                     /locus_tag="ACHJ48_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO4710546.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            507..581
                     /locus_tag="ACHJ48_005243"
                     /pseudo
     CDS             507..581
                     /locus_tag="ACHJ48_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          226 a          181 c          180 g          197 t
ORIGIN      
        1 taagcgtaaa ctgaccgccg tatgtagcca tcagacgaaa attggtaact tagacgccca
       61 tctgatatag acggacatct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
      121 aactattcga atgaattcaa acttcgtatg gtggaactgg catcacaacc aggagcctct
      181 gttgcccgta ttgcccggga acacgatatc aatgataacc tcctgttcaa atggctcagg
      241 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
      301 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc ggtggcggcc
      361 attgcgccgt ctttatccac ttccactcag accagtgtca gtgccggctc ctgcaaggtg
      421 gagttccgtc acggtaacat gacgctggaa aatccatcgc cagagctgct cacgctgttg
      481 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggtacc cgcatctggc
      541 tcgttgccga cgttaccgat atgcgtaaat ccttcaacgg atacgacata tgctgaagga
      601 ggagcagtaa tggattaagt gataacaaat acccagaaat agaggttcaa atccagctta
      661 gttagtacat aaatactatt ttgatgaaaa ttattatatg tttcctcaga ttttaatcag
      721 gcgcccagat taaaccaagt attgagcgcc tgattatgtt tattcattaa tatctgtcat
      781 tgat
//
LOCUS       DAZFDC010000427          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFDC010000427 DAZFDC010000000
VERSION     DAZFDC010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.430"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..153
                     /locus_tag="ACHJ48_005244"
     CDS             <1..153
                     /locus_tag="ACHJ48_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO4710547.1"
                     /translation="ISDIHVSISHIVVDNSVQPEESVSFSYESIIWEHCSAGTSAYSL
                     WEDRLF"
     gene            174..395
                     /locus_tag="ACHJ48_005245"
     CDS             174..395
                     /locus_tag="ACHJ48_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710548.1"
                     /translation="MANNILHSSTSDSESKNTSTDSTLFNEKFPFQLRQDFASRITED
                     ESDFFKWKKIRELAFVSNHKEWNKYDLLI"
     gene            474..>767
                     /locus_tag="ACHJ48_005246"
     CDS             474..>767
                     /locus_tag="ACHJ48_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFO4710549.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTID"
BASE COUNT          287 a          128 c          134 g          218 t
ORIGIN      
        1 ataagcgaca ttcatgtctc tatatcacat attgttgttg ataacagcgt tcaacccgaa
       61 gagtcagttt ctttcagtta tgagtcaatt atatgggagc attgttccgc tggcactagt
      121 gcatacagtt tatgggaaga tcgtcttttt taaaaacata tgggattatt ataatggcta
      181 acaatatttt acactcttca acatcagaca gtgaaagcaa aaatacaagc actgacagta
      241 cgttatttaa tgaaaaattc ccatttcaac taagacaaga tttcgcatca agaataacag
      301 aagatgaaag tgattttttt aaatggaaaa aaataagaga gttggcattt gtgagcaatc
      361 ataaagaatg gaataaatat gacttattaa tttaaaaatc tgttaatgaa atcaacattc
      421 atttgtccag cactccatac tttcagccgt tggattggta tataataaaa gccatgctct
      481 ggactgaaaa cgatgcggaa aatacttctc agtggaatgg ttatccatta caaattggtc
      541 gttttcggaa agataaagcg atgccagctt taatctctgg agaaaaatct acagcccttg
      601 ttaccccacc acaatggaga aacaaagcat tcaatgggtt aaaagatcca gaaagaaatt
      661 actgggcaaa agaacaaata actggctctc cagaagagaa tatcaaagca gcaattactt
      721 acttaatgat gaaactttct aatacaaagg aagaatcaac tatcgat
//
LOCUS       DAZFDC010000428          760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFDC010000428 DAZFDC010000000
VERSION     DAZFDC010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.431"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..137
                     /locus_tag="ACHJ48_005247"
     CDS             <1..137
                     /locus_tag="ACHJ48_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4710550.1"
                     /translation="LTGIRYEISQRKYIVMNPADYDKYSDMVKKHVIDGEEFIIKYYM
                     "
     gene            complement(275..604)
                     /locus_tag="ACHJ48_005248"
                     /pseudo
     CDS             complement(275..604)
                     /locus_tag="ACHJ48_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          216 a          163 c          172 g          209 t
ORIGIN      
        1 cccttacagg gattcgctat gaaatatcgc agcgaaaata tattgttatg aatcccgccg
       61 attatgataa atattccgat atggttaaaa agcatgtaat agatggtgag gaattcataa
      121 ttaaatatta tatgtaatta attaggcgta attgtgtcac tggaatagag gtttcttgtg
      181 acgcatgtgg aaggagcgtc tatggacaag gcgtttcctg ctggtgataa ataaaggcgc
      241 agcacgtcga atgcaagaat gcgctgcgtc tggctcatgg caattcatta tcttcgcata
      301 gcgtcaatga caaaaatcga ctgtgtggat caaattgata atcagcaaac ggttcgcaat
      361 caacgaaacc atgcttcaaa taaagttgat ggcaggccgt aaatccagcc tgtgtacccg
      421 tttctaagct caggcgatga aggcatctgt catgggcgac ctgcaaaatg tggcgtaaaa
      481 tcagactggc gacaccccga cgtaaataat ttggcgcggt acgcattgat ttcagttcgc
      541 catgtttatc atccagcaac tttagcgcac ccattcctgc gagttgttcg ccttcccata
      601 ctgacctgtt gacctggctt gtttaatgac atccgatctc accgcgtggg gttatggatg
      661 gggcaaactc actcaatttc tggtttaaaa tggaaacctg attctgaaag ttttctgcca
      721 cccacgatcc gtatacgcga taaaccctct gatcatcaga
//
LOCUS       DAZFDC010000429          728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFDC010000429 DAZFDC010000000
VERSION     DAZFDC010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.432"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>728
                     /locus_tag="ACHJ48_005249"
     CDS             <1..>728
                     /locus_tag="ACHJ48_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4710551.1"
                     /translation="LWKMAGVISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETS
                     QRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQA
                     AVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITD
                     VVAGTVEQVKLLLVQGDLALAEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRD
                     ELALRKSIAERDARIRQGEMGYINR"
BASE COUNT          187 a          150 c          245 g          146 t
ORIGIN      
        1 ttgtggaaaa tggccggggt gatcagcgat gaagccggtg gtggtatcag acaggcggca
       61 gaaaatctgg cccgtctggc agaaagcggg aaatataccg ccgggcagct acggatcatg
      121 ggggaaacct ctcagagatg gctgcagacg gtgggggacg atgccgggaa ggtggaaaaa
      181 gcctttgaag ggattgcagc agatccggtg aaggcgctgg cctccctgaa tcagcagtat
      241 aacttcctga gcgtttccca gttacgccat attgatgagc ttgagcgcac gaaaggtaaa
      301 caggctgcgg tgacggaggc gatgtccctg tttgcggatg tcatgaatgc acgtctggag
      361 caacttgata aagcggccac gccggtggaa aaaatctggg acgatgttaa aacctggact
      421 tctgacgcat gggcatggat aggtgatcat acactggggg cactcagtct gatcactgac
      481 gtggtggcag gaaccgttga acaggtgaag ctgctgcttg tgcaggggga tctggcgctg
      541 gctgaattta ttcagtcagc ctgggaaacg acaaagaatg tgcccggcgt tggtgcgttg
      601 tttggtgaac tggcagaaga gaaccgcgta tttattgaga aaacaaaacg tgatgaactg
      661 gcgctgagaa aatccattgc ggaacgggat gcgcgtatac gccaggggga aatggggtac
      721 atcaaccg
//
LOCUS       DAZFDC010000430          726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFDC010000430 DAZFDC010000000
VERSION     DAZFDC010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.433"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..205
                     /locus_tag="ACHJ48_005250"
     CDS             <1..205
                     /locus_tag="ACHJ48_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4710552.1"
                     /translation="NGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVT
                     DSAEQPQSITSDACTVTVNGAGG"
     gene            211..585
                     /locus_tag="ACHJ48_005251"
     CDS             211..585
                     /locus_tag="ACHJ48_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO4710553.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            609..>726
                     /locus_tag="ACHJ48_005252"
     CDS             609..>726
                     /locus_tag="ACHJ48_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4710554.1"
                     /translation="MRLALRLGRTLSELRHSLSASEAMMWMEFDRVSPLGDER"
BASE COUNT          165 a          171 c          244 g          146 t
ORIGIN      
        1 caacggggga acgccgcctt ataaacacgc ctggaagaag gatggtcagc cggtagaggg
       61 acagactact gacactttca gtaaagccaa tacgcagtca ggtgataagg gggcttatac
      121 ctgcgaggta acggattctg cagaacagcc gcagagcatt acctctgatg cgtgtacagt
      181 aacggttaat ggtgcgggcg gataaggctt atggcaaaag atctgaaaac actggcgctg
      241 gccagactgt cggggttccg tcataaaacg gtgaaggtgc cggaatggag aaatgtcagc
      301 gtggtgctgc gggagccttc ggcagaggcc tggtatctgt ggcaggaagt gctcaatggt
      361 gatggagagg atgacgatac cctgtcggtg gtggcgaaaa cccgccgtaa cctggaagcg
      421 gatgtgacgc tgttctgcga tgtcctgtgt gatactgacc tgcaacgggt gttcactccg
      481 gacgaccgtg agcaggtgct ggccgtctat ggtccggtac atgcccggtt gctgcgtcag
      541 gcactggaac tgatcgctga tgcagagtcg gccagaaaaa agtagcccgc ccggaaattc
      601 gctttctgat gcgacttgcg ctccgtctgg ggcgcacctt atccgaactg cggcacagcc
      661 tgagtgcgag cgaggcgatg atgtggatgg agttcgacag ggtatccccg ctgggtgatg
      721 agcgcg
//
LOCUS       DAZFDC010000431          721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFDC010000431 DAZFDC010000000
VERSION     DAZFDC010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.434"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>721)
                     /locus_tag="ACHJ48_005253"
     CDS             complement(<1..>721)
                     /locus_tag="ACHJ48_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4710555.1"
                     /translation="RDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQA
                     VFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNK
                     VEWRSWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGG
                     VYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMN
                     GSAGLVYSPVYHIAIQNDGANG"
BASE COUNT          140 a          239 c          172 g          170 t
ORIGIN      
        1 ccgttagccc cgtcattctg aatggcgatg tggtagaccg gggaatacac cagaccagca
       61 ctgccgttca tactgcccac ggcgcgtacg cccagcgagc catccgccgc ccgggtcagg
      121 ggcataatag cttcaggtcc ggcttccccc atcagcccgg ccccttttgc aaacgcaaag
      181 tacgtgggcg tgtccacaat gctgttgctg tacgcgctca ggtttgccga ggtatacacg
      241 ccgccttttg cattggccac cgctccgccc agccagtcac caatgctgcc gagaaatcct
      301 cccgcaccgg acataccgtt tgccgccgtc ttaattccgt tgacaatcgc ggcattcata
      361 agaacttttg atatttcctg cagcactgat gaggcccagc tgcgccattc cactttattt
      421 ccgttcagca tctccgtgat gttattcacc atccctgaga taccctccgt cgccagctgt
      481 gctgcctgtg aggcgtaatc ggacgcatta tccacccagt tactgaatcc ctcctgcagc
      541 cctttctgcc agtccgcacg ctgcacatcc gattcggcat aaaagactgc ctggtcctta
      601 aggcgttcgc tcagatactg cgcgttctgt gccagagcct gtctgtaaaa atcctcactg
      661 atatccccgg tctgatactg agactgaagg tccgcatcct tctggcggaa gctgtcgcgg
      721 a
//
LOCUS       DAZFDC010000432          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFDC010000432 DAZFDC010000000
VERSION     DAZFDC010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.435"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(493..696)
                     /locus_tag="ACHJ48_005254"
     CDS             complement(493..696)
                     /locus_tag="ACHJ48_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4710556.1"
                     /translation="MQSPTAQLRLGPADILESDENGIIPEQDRVITQVVILDADKKQI
                     QCVVRPLQILRADGTWENIGGMK"
BASE COUNT          195 a          180 c          135 g          202 t
ORIGIN      
        1 ggcagcagag tatccagcag gggcaaaaaa gttgaagcca ggttggagaa cgggaatttt
       61 catattgata cttaatatgg ttttatatat ttcagtgcca ccaattcact agcttacaac
      121 cagaaactaa aaaaacgtca gggcgttgat aataatgaat accaactttc tgcccattta
      181 tagtaatatc tttcgttatc aggttttgtc tggcagcttc acgtaatatt tcaatactct
      241 gcaaagcaac atttgatgaa atgctggatg ttatcaatgg catatgtcat ttaccccaac
      301 aagactaaat tttagaaaaa acaactgccg ggacaacttt cgccccctgt aaccccaaat
      361 aagatgtcag atttatcggc attaatttat ctccctgttg aattagctcc tctatcatct
      421 acttaacaaa tatcccccgg acattgcaac acaaaaaccg gagccggact ccggttttgt
      481 gaagctgtcg ggttacttca tcccgccaat attttcccac gtcccgtcag cacgcaggat
      541 ttgcagcggt cttaccacgc actgtatctg ctttttatcc gcatccagta tcaccacctg
      601 cgtgattacc ctgtcctgct ccgggataat gccattctca tctgactcca ggatgtctgc
      661 cggtcccagt cgcagctgtg ctgtaggcga ctgcacgtgt tcacggccat ca
//
LOCUS       DAZFDC010000433          676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFDC010000433 DAZFDC010000000
VERSION     DAZFDC010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.436"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>676)
                     /gene="espX2"
                     /locus_tag="ACHJ48_005255"
     CDS             complement(<1..>676)
                     /gene="espX2"
                     /locus_tag="ACHJ48_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HFO4710557.1"
                     /translation="MKIELTEGSGFSKSLSDSQIEMHILRTVNSLNVGEKLNDDNLAD
                     HSIFTNEFSVIIPPSYYDATSAVNANNIVREKLFESDSKVKDIVDDMSNHDVESEKDI
                     FVIGGMIEKLNSLADESFNDSTDNIQTVKDLLTQLTDGMELFALRDIVAFPSTIIAKL
                     IKSPLNSDHELVMRALDTYLCYFRNKNLNNNAEIINFFHALFLKRPELMVAENYRFIQ
                     FIDLLF"
BASE COUNT          212 a          131 c          105 g          228 t
ORIGIN      
        1 tcaaaaagca aatcaataaa ttgaataaat ctataatttt cagcaaccat caactctggt
       61 ctctttaaaa agagggcatg gaaaaagtta attatttccg cattgttatt cagattttta
      121 tttctgaagt agcacagata cgtatccagt gcgcgcataa caagttcatg atcgctatta
      181 agtggggatt ttattaattt tgctattatg gttgatggga atgccactat gtcccgcagc
      241 gcgaagagct ccatgccatc agttaattgc gttaacagat cttttactgt ttgtatgtta
      301 tcagtactat cattgaaaga ttcgtctgcc agagaattca gtttttctat catccccccg
      361 attacaaata tatctttttc gctctccacg tcatgattag acatatcatc gacgatatct
      421 ttaactttac tatcactttc aaatagtttt tctctcacaa tattattagc gtttactgca
      481 gaagttgcat cataatagga tggcggaata ataacagaga attcgttagt gaatatagaa
      541 tgatctgcaa gattatcatc attcaatttc tcgccaacat ttagcgaatt aacagtcctc
      601 agaatatgca tctcaatctg actgtcagaa agacttttag aaaaaccact accttcagtc
      661 agttcaattt tcatcc
//
LOCUS       DAZFDC010000434          663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFDC010000434 DAZFDC010000000
VERSION     DAZFDC010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.437"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..>663
                     /locus_tag="ACHJ48_005256"
     CDS             10..>663
                     /locus_tag="ACHJ48_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO4710558.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTKCHGESLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     MKEDNGNVSQTAKSPAERKRAQRERERLREQNTDCHDESRRVTHMSRQVTTDTDTDKE
                     LNPTHNARMRESAPASESNGAPLQTAEPEYPDGLSEPIGKFPMTGVWRPSLDFRQRAA
                     "
BASE COUNT          181 a          154 c          199 g          129 t
ORIGIN      
        1 gaggtgagta tggctaatgc ctggctcaga ttgtggcatg acatgccaaa tgatcccaaa
       61 tggcgaacca ttgccagggt ctcaggacag ccaatcgcaa cagtgatggc ggtgtatatc
      121 caccttctgg tgagtgcgtc acgaaatgtc acgaaatgtc acggcgagtc actacgtggt
      181 cacattgatg tcacgacgga agatttagca agtgcgcttg atgtgacgga agacgtgatt
      241 gattcaattt tgcatgcaat gcaggggcga gttctggatg gcgatcttat ttccggatgg
      301 gaaaaacgcc aggtgatgaa ggaggataac ggtaatgttt cgcaaaccgc aaaatccccg
      361 gcagagcgca agagagcgca gcgggagcgg gaaagactgc gggagcagaa cactgattgt
      421 cacgatgagt cacgacgcgt cacgcatatg tcacgacaag tcacgacaga tacagataca
      481 gataaagaat taaaccccac acataacgcg cgcatgcgcg agagtgctcc agccagtgag
      541 tcgaatggcg cgccgttgca gacagcagaa cctgaatacc cggatggcct gagcgaaccg
      601 atcgggaaat ttccgatgac tggtgtctgg cggccgtcgc tggattttcg acagcgggct
      661 gca
//
LOCUS       DAZFDC010000435          648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFDC010000435 DAZFDC010000000
VERSION     DAZFDC010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.438"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>648
                     /locus_tag="ACHJ48_005257"
     CDS             <1..>648
                     /locus_tag="ACHJ48_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO4710559.1"
                     /translation="PANHAAQFGALVDKFRADLADMAGQCVGGSADGVPWICGDTTYF
                     WKQKNEATYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSK
                     WRDSSATWTSQDRASHFSAWARRGIISDRLATAILRHAGRVALNAGASSTVSEVRPSS
                     PSGAEATGITTLLSYLASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEA"
BASE COUNT          166 a          161 c          208 g          113 t
ORIGIN      
        1 gccggcaaat cacgcagcac agtttggtgc gctggttgat aaattccgtg cagacctggc
       61 ggatatggca ggtcagtgcg tcggtggctc tgctgacggt gttccctgga tatgcgggga
      121 cacgacgtat ttctggaagc agaagaacga agccacctac cagacggtgt acggcagcta
      181 caaaaacaaa acggaaaaaa atatccattt cgtaccgttc atgacggatg agaacggggt
      241 gaatgtgccg acgaacaaac cggaagaaga cccggacatt ccgggtatcg gatattacgg
      301 ttcgaaatgg cgtgacagct cagccacctg gacgtcacag gacagggcga gccatttcag
      361 cgcctgggca cgccgtggga ttatttccga ccgtctggca acggcgattt tgcgccatgc
      421 gggaagagtg gcgctaaacg cgggggcatc atcgacagta tcagaggtgc gcccgtcatc
      481 gccttccggt gcagaagcca caggcatcac aacactgctc tcttaccttg ccagcgagtc
      541 agagggaagc ctgaaagtac agggatggtc agccagtggc ggcagggcag aagtggtcag
      601 cgatgcggag ggaaccggag gtaaggcagt gaagctgacc aaggaagc
//
LOCUS       DAZFDC010000436          647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFDC010000436 DAZFDC010000000
VERSION     DAZFDC010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.439"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>647
                     /locus_tag="ACHJ48_005258"
     CDS             <1..>647
                     /locus_tag="ACHJ48_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710560.1"
                     /translation="QKIREGWQPYGGPFSSYTDDGAALIQAIVAEGDVSTPVVVKLTG
                     GEGAVISATRDPEYYFIVVLAGQSNSMAYGEGLPLPETYDRPDPRIKQLARRSTVTPG
                     GVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIPANA
                     GILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALKK"
BASE COUNT          155 a          154 c          202 g          136 t
ORIGIN      
        1 caaaaaattc gtgaaggatg gcaaccatac ggtgggccgt tttcttcgta tacggatgat
       61 ggcgcagcac ttattcaggc gattgtcgca gaaggtgatg tgagcacacc tgttgtggtg
      121 aagctgacag gtggagaagg tgcagtaatc agcgccacca gagacccgga gtattacttt
      181 attgtggttc tggcggggca gtcaaacagc atggcatatg gtgaaggcct tccgctgccg
      241 gagacatatg accgtccgga cccgcgtatt aagcagctgg cgcgccgcag tacggtgaca
      301 ccgggcggtg tcgcctgtaa atataacgac atcattccgg cggaccattg tctgcatgat
      361 gtgcaggaca tgagccgcct taaccatccg aaagcggacc tgtcaaaggg gcagtacgga
      421 accgtggggc aggggctgca tatcgccaaa aaattgctgc cgtttatacc ggcgaatgcg
      481 ggcattctgc tggttccgtg ctgtcgtggt ggttcagcgt tcaccaccgg agctgatggc
      541 acatacagtg acgcgagtgg tgcttcggag aattcaaccc gctggggtgt ggacaagccg
      601 ctgtataagg accttatcgg tcgaacaaaa gcagcactga agaagaa
//
LOCUS       DAZFDC010000437          642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFDC010000437 DAZFDC010000000
VERSION     DAZFDC010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.440"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..161)
                     /locus_tag="ACHJ48_005259"
     CDS             complement(<1..161)
                     /locus_tag="ACHJ48_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4710561.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALA"
     gene            complement(142..567)
                     /locus_tag="ACHJ48_005260"
     CDS             complement(142..567)
                     /locus_tag="ACHJ48_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4710562.1"
                     /translation="MAREMGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFS
                     GLMYAVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGD
                     GGRDISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
BASE COUNT          143 a          211 c          145 g          143 t
ORIGIN      
        1 gccagcgcct gtcggctcag cccctgttca acagtactgg cggtttttct ggcatccgcc
       61 tccagactgg aaaaatgacg gcgtacccgg gccatctgtt catcaaaccg ggccgcatcc
      121 agactcaaat caataaccag atcacccgct ggctgggaca tatctcacac ctccggaaat
      181 ccccgctgaa gccatcatta atgcaacatc atcctcgctg acatccacca catccgcaga
      241 aggtgaaata tcgcgccctc cgtcccctcc gaaccggacg cctccggcaa ctcctgtcgc
      301 tttctgcatc agcatttctt cctcgtccgg catctccgtc tgctcttcct cacacgctgg
      361 agcaagcaga ctgaaatccg ccggatgcat atccggatcg ccaaaaaaca ggctgagtac
      421 ggcgtacatc agcccggaaa aatgagcgtc cagttgggta tcctgaaaat aatgcgtgca
      481 gtaaaaacgt cgccagtcgg catattcggt ggatgtcatc ccggcaagca tggcgcgcca
      541 gtcgggtctc cccatctctc gcgccagttt caggacaaag ttcagctcgc cttcgaatgc
      601 ttttttgatg ttaccgactc agtcgcttct gctttaccgg tt
//
LOCUS       DAZFDC010000438          621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFDC010000438 DAZFDC010000000
VERSION     DAZFDC010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.441"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>621
                     /locus_tag="ACHJ48_005261"
     CDS             <1..>621
                     /locus_tag="ACHJ48_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4710563.1"
                     /translation="ALDRLVQQRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESD
                     RLTHRTVNGEPAEQWQYDEHGWLTEISHLSEGHQVAVHYGYDDKGRLAGERQTVHNPE
                     TGELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPLVEFTRDR
                     LHRETVRSFGNNAYELTSTYTPAGHLQSQRLNSQVYDRDYDWNDNG"
BASE COUNT          147 a          177 c          207 g           90 t
ORIGIN      
        1 tgcgcttgac cggctggtac agcagcgcgg ctttgacggg cggacgcaac gttaccacta
       61 tgacctgacc ggaaaactca cgcagagtga agatgagggg cttgtcaccc tctggcacta
      121 cgacgaatcg gaccgcctca ctcaccgcac ggtgaacggc gaaccggcag agcagtggca
      181 gtacgacgag cacggctggc tgacagaaat cagccacctg agcgaaggcc atcaggtggc
      241 ggtgcattac ggttatgatg ataagggccg cctggccggg gagcgccaga cggtgcataa
      301 cccggagacg ggggaactgc tgtggcagca tgagacagag cacgcataca acgaacaggg
      361 tctggcaaac cgcgtcacgc cggacagcct gccgcgggtg gagtggctga cctacggcag
      421 cggttatctt gcggggatga agctgggcgg gacgccgctg gtggagttca cgcgcgacag
      481 gctgcaccgc gagacggtgc gcagcttcgg caataacgca tacgaactga ccagcacata
      541 cactcccgca ggccatttac agagccagcg cctgaacagc caggtgtatg accgtgacta
      601 cgactggaat gacaatggcg a
//
LOCUS       DAZFDC010000439          617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFDC010000439 DAZFDC010000000
VERSION     DAZFDC010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.442"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>617
                     /locus_tag="ACHJ48_005262"
     CDS             <1..>617
                     /locus_tag="ACHJ48_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO4710564.1"
                     /translation="QSAIIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGI
                     EDTDGQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSA
                     NPPTFSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKT
                     VGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAK"
BASE COUNT          157 a          156 c          174 g          130 t
ORIGIN      
        1 cagagcgcca tcatacagca gatacagcgc gtgcagaagg acacaaatga tgaccttgct
       61 gcactttaca tgctgaaggt acagaaaaca aaaaatggca taccctatgt tgccggtatt
      121 ggagcgggga ttgaggatac tgatggccag cccctgagca acatactgct gctggctgac
      181 cgtattgcga tgattaaccc ggaggacggc aacaccacgc cgttatttgt ggcgcagggg
      241 aatcagttgt tcatgaacga tgtgttcctg aaacgactgt ttgcggtgag tatcacgtca
      301 tccgccaatc ccccgacgtt ttccctgacg ccggagggca ggctgaccgc aagaaatgct
      361 gatatcagcg gtaacgtgaa tgcgaattcc gggacgctca acaacgtcac gattaacgag
      421 aactgtcggg ttctgggaaa actgtccgcg aaccagattg aaggcgatct cgttaaaaca
      481 gtgggcaaag ctttcccccg ggattcccgt gcaccggagc ggtggccatc agggaccatt
      541 accgtcaggg tttatgacga tcagccgttt gaccggcaga ttgttattcc ggcggtggca
      601 ttcagcggcg ctaaaca
//
LOCUS       DAZFDC010000440          614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFDC010000440 DAZFDC010000000
VERSION     DAZFDC010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.443"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..267
                     /locus_tag="ACHJ48_005263"
     CDS             <1..267
                     /locus_tag="ACHJ48_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO4710565.1"
                     /translation="TILSGAAVVVGSMVAVAITDIPAGEAGDGFAEGVFLLPKQSADD
                     IQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATVAVKINV"
     gene            260..541
                     /locus_tag="ACHJ48_005264"
     CDS             260..541
                     /locus_tag="ACHJ48_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO4710566.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            544..>614
                     /locus_tag="ACHJ48_005265"
     CDS             544..>614
                     /locus_tag="ACHJ48_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO4710567.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRA"
BASE COUNT          116 a          146 c          215 g          137 t
ORIGIN      
        1 accatcctga gcggtgctgc ggtggtggtc ggcagtatgg tggccgtggc cattaccgat
       61 attcctgccg gtgaggccgg tgacggtttt gccgaaggcg tgttcctgct gcccaaacag
      121 tctgctgacg acattcagtc cggcgcggtg gtttatctga aggacggggt tgtgcagctg
      181 gctgcagacg gtgcggtggc agcgggggta gcctgggaaa atgcccctgc aaacagcgcc
      241 actgtggcgg taaaaatcaa tgtctgatct gtttacgcga atgtgttgcc ggatggacgt
      301 ggcgaccgtt cgggtgatgg gcaaacaggc ggagattaac ggcgtcgtgt acgacgtgat
      361 gccggaggaa gagtccgcgg agatgggggc gctttcgggc agccagttgt cactggtggt
      421 gttttcagcc cggtaccgtc cggcccgtca tgatgttgtt gtgtttgcgg ggcgcacact
      481 gacggtgacc cgttatgaca cgtacaacgg taaaccccgg atttttgtcg aacaggaatg
      541 agtatggcaa taaaaggtct ggcgcaggcc atgaaaaatc tggatgcaat tgatcgccgt
      601 gccgttcccc gggc
//
LOCUS       DAZFDC010000441          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFDC010000441 DAZFDC010000000
VERSION     DAZFDC010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.444"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHJ48_005266"
     CDS             <1..198
                     /locus_tag="ACHJ48_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO4710568.1"
                     /translation="MLANTCMWTYRSDECGYTGGAVADEFDNPTTDIRKDRCSKCMRG
                     CEMRSMVANFGGFLSINKLSQ"
     gene            209..>608
                     /locus_tag="ACHJ48_005267"
     CDS             209..>608
                     /locus_tag="ACHJ48_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4710569.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAG"
BASE COUNT          119 a          147 c          205 g          137 t
ORIGIN      
        1 atgctggcga atacctgtat gtggacctac cgctctgatg agtgtggtta cacgggcggg
       61 gcagtggcgg atgagttcga caaccccacc acggatatcc gtaaggacag atgcagcaag
      121 tgcatgcgcg ggtgtgagat gcgcagcatg gtggctaatt ttggcggttt cctttccatt
      181 aataaacttt cgcagtaaat cccggtttat gacacagact gaatcagcga ttctggcgca
      241 tgcccggcgg tgtgcgcctg cggagtcgtg cggcttcgtg ataagcacgc cggaggggga
      301 gtggtatatc ccttgtgtga atatttctgc agagccggag gcgtattttc gtatcgcacc
      361 ggaagactgg ctgcgggcag agatgcaggg ggagattgtg gcactggtcc acagtcatcc
      421 cggtgggctg ccctggctga gcgaggctga ccggcggctg cagataaaaa gcgcactgcc
      481 ctggtggctg gtctgccggg gtgacattca caaattccgc tgtgtgccac atctgacagg
      541 acggcgcttt gagcacgggg tgacggactg ttacacgctg ttccgggatg cttatcatct
      601 ggcgggga
//
LOCUS       DAZFDC010000442          598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFDC010000442 DAZFDC010000000
VERSION     DAZFDC010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.445"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..413)
                     /locus_tag="ACHJ48_005268"
     CDS             complement(<1..413)
                     /locus_tag="ACHJ48_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO4710570.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKG"
     gene            complement(472..>598)
                     /locus_tag="ACHJ48_005269"
     CDS             complement(472..>598)
                     /locus_tag="ACHJ48_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710571.1"
                     /translation="QIRQDAREAIMLLAPGSVSEMQDYIPENRCYRATLEFQVTV"
BASE COUNT          142 a          166 c          153 g          137 t
ORIGIN      
        1 cccttcaggc gaagtgaaaa cgttgcagcc accacgccgt tggtaccgga tgaccaggtg
       61 tgctgacgca cttccgccag gaacttaaag cccttaccgg acggaaactg caccttaaac
      121 gcatacaccg tgtcattgtc ataggcatca cgcagggcgt tctgggcctg attcagataa
      181 aaattacccg acatggaaat ctcggacgac gcccccagac cgttgatgtt ctcctgctct
      241 gtggagcaga gcgtggtcac atcaatatcc tgtttctgac cggcggtgaa ctggacttcc
      301 ttgatggtgc agtccaggcg cagatattcc gccttctcca tggtttcagc agtcgccggg
      361 gcagatgaaa tcatcacctg cgtcagctgt gaacgttcat acaaagcaga cattctgcct
      421 cctgataata aaaaacccgc acgcggcggg ttatgggttc tgttgaaaaa attacaccgt
      481 gacctgaaac tccagggttg cacggtaaca gcggttttcc ggaatatagt cctgcatttc
      541 actgacggat cccggggcca gcagcattat ggcttcacgg gcgtcctgac gtatctga
//
LOCUS       DAZFDC010000443          596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFDC010000443 DAZFDC010000000
VERSION     DAZFDC010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.446"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            82..>596
                     /locus_tag="ACHJ48_005270"
     CDS             82..>596
                     /locus_tag="ACHJ48_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO4710572.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVT"
BASE COUNT          179 a          109 c          141 g          167 t
ORIGIN      
        1 tggcggacaa ttattgttat tattaagtct aatgtcattt aaaggcatta tcatatttac
       61 gaagagatca ggatgataac gatgaaaaaa agtgtattga cggcgtttat aactgtggta
      121 tgtgcaacgt ccagcgttat ggctgctgat gataatgcta tcacggatgg ctcagtaaca
      181 tttaatggta aagttattgc tccagcttgt accctggtag ctgcgacgaa agattccgtg
      241 gtgactttgc cagatgttag tgccacgaag ttgcaaacca atggtcaggt ttctggcgtg
      301 caaactgatg tgccaattga attaaaagat tgtgatacta ccgtaacaaa aaatgcaacg
      361 ttcaccttta atggcactgc ggatactact cagattacag cgtttgctaa ccaggcctca
      421 tctgatgctg ctacaaacgt ggccctgcaa atgtatatga atgatggtac aacggccatc
      481 aagccagaca cagaaaccgg gaacattttg ttgcaagatg gagatcagac gttgactttt
      541 aaagttgatt atatcgctac ggggaaagcg acttctggta atgtgaatgc ggtaac
//
LOCUS       DAZFDC010000444          595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFDC010000444 DAZFDC010000000
VERSION     DAZFDC010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.447"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..386
                     /locus_tag="ACHJ48_005271"
     CDS             <1..386
                     /locus_tag="ACHJ48_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFO4710573.1"
                     /translation="TRLNEAKCLLDHGFFHGAYYLCGYAVECALKACIAKSFLQHEFP
                     NKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWSEQFRYDNNISK
                     AMAEQLFDAVGDQNSGVLKWVKAHW"
     gene            365..>595
                     /locus_tag="ACHJ48_005272"
     CDS             365..>595
                     /locus_tag="ACHJ48_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710574.1"
                     /translation="MGKSTLVIGRELTKDMEFSGQFLLKKLKLQNLTIDAAMWFYYPD
                     LSWRYILVISDFSERGPAEIYRKISEINRNSIS"
BASE COUNT          197 a           90 c          123 g          185 t
ORIGIN      
        1 acactaggct taatgaggcc aaatgcttgc ttgatcatgg cttttttcat ggtgcatatt
       61 atctttgtgg gtatgcagtt gaatgtgcat tgaaggcctg tattgccaag tcatttttac
      121 aacatgagtt tccaaacaaa aaagtcgtaa atgattcata tactcatgat ttgtcgcaac
      181 ttctcaaaat tgccaactta catcaaattt tgattgctga cgcaaaaaat gatgtttcgt
      241 tggagattaa ctggtcggtc gttaaagact ggagcgagca atttagatac gacaataaca
      301 taagtaaagc tatggccgaa caattgtttg atgctgtagg tgaccaaaat tctggagttt
      361 tgaaatgggt aaaagcacac tggtaatcgg cagagagttg acaaaagata tggaattctc
      421 aggtcaattt ttgttaaaaa aactcaagtt acagaattta actattgatg ctgcaatgtg
      481 gttttattac ccagatctat cttggagata tattttagtt atcagtgact tctcagaacg
      541 tgggccggca gaaatatata gaaaaatcag tgagataaat agaaatagca tatca
//
LOCUS       DAZFDC010000445          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFDC010000445 DAZFDC010000000
VERSION     DAZFDC010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.448"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..534
                     /locus_tag="ACHJ48_005273"
     CDS             <1..534
                     /locus_tag="ACHJ48_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO4710575.1"
                     /translation="IFTIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSPGSLPFTT
                     AATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWI
                     YLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAA
                     KQHYKNAERLLAGLPAH"
BASE COUNT          188 a          135 c          125 g          145 t
ORIGIN      
        1 atattcacaa tttttatagt tgcagcaatt acctgtatta gccaacccaa gaaaacgacg
       61 ttgcgtgata aagccatggt gaattatgcc tttgattatt taagctcacc gggcagtctt
      121 ccattcacca cggcagccac ggagctttcc gcgattcatg gtcactcaac gtcgcaatat
      181 cgccttggag aattttatct tcatggtagc gacggtaaac cactggatta tacacaggcg
      241 agatactggt atgagcaatc agcggaacag gaaaatccac gcgcgcaaag taaactgggg
      301 tggatctacc tcaaaggtct gggggtcaaa cccgacaccc gtaaagcaat tctctggtat
      361 aaggaagcag ctgaacaagg gtatgctcat gctcaatata ctttaggttt gatctacaga
      421 aatggctcag gtattaatgt taaccattat gaatctcaaa aatggttaaa actggccgcc
      481 aaacaacatt acaaaaatgc ggaaagatta cttgccgggc ttcccgcaca ttaacgtcga
      541 ctgtcacccg aacagatgga gtattttccc tatcaaacca gcaaagataa aaa
//
LOCUS       DAZFDC010000446          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFDC010000446 DAZFDC010000000
VERSION     DAZFDC010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.449"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..81)
                     /locus_tag="ACHJ48_005274"
     CDS             complement(<1..81)
                     /locus_tag="ACHJ48_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710576.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIA"
     gene            143..593
                     /locus_tag="ACHJ48_005275"
                     /pseudo
     CDS             143..593
                     /locus_tag="ACHJ48_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          167 a          148 c          142 g          136 t
ORIGIN      
        1 cgctatttca ctgaagcctg ttccgttctt ccatagttca aaaacagagg ctttttcctc
       61 tgctgtaaat gttcgtctca ttcaaaaaac ctccgcaacc ccatgttttc acataactgt
      121 tgcgttgacc aattgaatct acaacagcgt attttgcaca ggagtcgctg aaaaatacgc
      181 gttaatcgaa caatggcgac aacaatttcc cattgaagcg atgtgtcagg tatttggtgt
      241 atccaggagc ggttattaca actgggtaca gcatgaaccc tcagacagaa aacaaagtga
      301 tgagcggcta aaactggaga ttaaggtggc acatatccgc actcgcgaaa catatggaac
      361 ccggcggctc cagacggagc tggcagagaa tggcatcatc gttggtcgtg accgactggc
      421 acgtcttcgt aaggagctaa ggctacgctg taagcagaaa cgcaagttca gggcgactac
      481 gaactcgaac cacaatctgc cagttgcgcc aaatctgctg aaccagacgt tcgctcctac
      541 agcaccaaat caggtctggg tggcggacct gacgtatgtt gccacacagg agg
//
LOCUS       DAZFDC010000447          591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFDC010000447 DAZFDC010000000
VERSION     DAZFDC010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.450"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>591
                     /locus_tag="ACHJ48_005276"
     CDS             <1..>591
                     /locus_tag="ACHJ48_005276"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO4710577.1"
                     /translation="PGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQNYALNGYSR
                     GTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYR
                     TGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNS
                     EDKLIVFHLMGSHGPTYYLRYPESHKYFKPTCDRSD"
BASE COUNT          201 a           98 c          119 g          173 t
ORIGIN      
        1 ctcctggcga gtttcgtcag actggaacgg atgcatcccg cacaataaat gaaaaacaaa
       61 agccggtaat tatgttttta gttgtcgggg agacagcaag aagtcaaaac tatgctctga
      121 atggatattc tcgtggtacc aatgatttta caaagaaata taatgaactt atatcctttc
      181 ataatgtgca gtcgtgtggc acatccacag ccatatctgt tccatgcatg ttttcagata
      241 tgaagcgaaa agagttcaat tcccgcaaag cggtaaattc ggaaaatgta ctggatattc
      301 tgtacagaac gggggtaaat ttactctgga ttgaaaatga cgggggatgt aaaggtgtat
      361 gtaagcgaat ccctacaatt aatattgagc catcaaattc agataataca ttatgcaaga
      421 aaaactcatg ttatgatgag gttatgctaa aaaatattga tgagtatata aataataact
      481 cagaggataa acttatcgta tttcatttga tgggtagtca tggtcctacc tactatctcc
      541 gttatcctga gtcacataaa tattttaaac caacatgtga tcgtagtgat a
//
LOCUS       DAZFDC010000448          582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFDC010000448 DAZFDC010000000
VERSION     DAZFDC010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.451"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..247
                     /locus_tag="ACHJ48_005277"
     CDS             <1..247
                     /locus_tag="ACHJ48_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HFO4710578.1"
                     /translation="GKLFKKLDTSPYKMSIILALAIDCERNNANIIIDSEYKSNKAVK
                     EYVEILKEFNICPEKVAEYLSEFSGKHFLDVYNYYSN"
     gene            429..>582
                     /locus_tag="ACHJ48_005278"
     CDS             429..>582
                     /locus_tag="ACHJ48_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO4710579.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRIIYFSRTV
                     VRNKKVI"
BASE COUNT          212 a          100 c          105 g          165 t
ORIGIN      
        1 cggaaagtta tttaaaaaac ttgataccag tccatataaa atgtcaatta tcctagcatt
       61 agcaattgat tgcgagagaa ataatgcaaa cataattatt gatagtgagt acaagtcaaa
      121 taaggcagta aaggaatatg tagaaatttt aaaagaattc aatatatgcc cagaaaaggt
      181 agcagaatac ctaagcgagt tcagcggaaa acatttcctc gatgtatata attattattc
      241 caattgaaaa aaatataaag tatctatcac ggcactctca gttgagtatc acaggcacat
      301 gtattttagc cccctgagtc tacagacaac caatattact taaataagtg ataatcttaa
      361 tgctgttttt tagaacagat gattgatatt tttgggccgg agaaacgcag acaggctttg
      421 ttgaataaat cgagcttttg ctgagtttaa ggatcagatc actcattttc ctgcaaaagc
      481 tcattgggcg aaacaatctg actcagagaa cccggattaa acgtctggcc cgcagaatca
      541 tctatttctc acgcactgta gtacgtaata aaaaagtcat cg
//
LOCUS       DAZFDC010000449          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFDC010000449 DAZFDC010000000
VERSION     DAZFDC010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.452"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>571)
                     /locus_tag="ACHJ48_005279"
     CDS             complement(<1..>571)
                     /locus_tag="ACHJ48_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO4710580.1"
                     /translation="GDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDM
                     KMDKQEVKREYKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIY
                     FKPHLSPIPLISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFEN
                     IDEILRLLLWLEDVENAGQPVPDEQLSSED"
BASE COUNT          174 a          111 c           90 g          196 t
ORIGIN      
        1 atcttctgaa gagagctgtt catctggaac aggttgtcca gcattctcca catcttcaag
       61 ccacagcaga agacgtaata tttcatctat attttcgaag ctaacataat cgtagcgacg
      121 atgggtagca taaatttttc gtgctaattt tttatctgta ataattggta tcccgatttc
      181 ctttgcatat ttccttacag ctaatgctac ctcattagtt tctcttacag aaatcaatgg
      241 aataggtgac agatgtggct taaagtatat ccctattgca atgtgagtag ggttggcaat
      301 catcaaacgg ctattactga catcagattt caattgctca gaaagaattt cctgatggcg
      361 ctctctgcgt ttagacttaa tttcaggatt tccttcttgt tccttgtatt ctcttttaac
      421 ttcttgttta tccattttca tatctttcat aaataaaaaa tattcagcga taaaatcaaa
      481 aattaagaca attatcatac tgccgagaca atacagtatg agaaggaata gcatctctcc
      541 ccaatctgat aataaagata tgatatctcc a
//
LOCUS       DAZFDC010000450          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFDC010000450 DAZFDC010000000
VERSION     DAZFDC010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.453"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..394
                     /locus_tag="ACHJ48_005280"
     CDS             <1..394
                     /locus_tag="ACHJ48_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO4710581.1"
                     /translation="EEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMSVLARELV
                     ASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDGEIRRYDR
                     QLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT          180 a           95 c          158 g          138 t
ORIGIN      
        1 ggaagagcgg gaaaaaatta aagggcaggt tgttgagcta gtacgccgga gtgggcgcga
       61 gacgttacgg caactggaag ccaagacagg tgcgacaaga tatctgatga gcgttcttgc
      121 cagagagctg gttgccagtg gcgatgtata caactctggt tacgggttat tcccatctga
      181 acaggctcgt aaggactggc aaaacgcccg caaaaaacta tcgagggcaa aactgaagaa
      241 accggttgtg gttgatcctg accttatctg gtcattacca gacggagaaa tacgccgcta
      301 tgacaggcaa ttgaacataa tttgtagtga gtgccgtaac agtgaagtga tgcagcgagt
      361 tttgatattt tacacaggag taatgatgga atagtgaaaa taacatgaaa cctttggatt
      421 ctggtgtttc agtggatggg aaaagagcag atacatagaa aatgaatagc aataattcac
      481 agtctggatg ttgtttgtat gcttataaaa tgatcctagt atgcattgcc ggagttgaat
      541 cagctccaat gaagaaactg agaggagtat t
//
LOCUS       DAZFDC010000451          561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFDC010000451 DAZFDC010000000
VERSION     DAZFDC010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.454"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>561
                     /gene="rfaY"
                     /locus_tag="ACHJ48_005281"
     CDS             <1..>561
                     /gene="rfaY"
                     /locus_tag="ACHJ48_005281"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO4710582.1"
                     /translation="DNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVL
                     KVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHY
                     YIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRL
                     IDLSGKKTTAVLKAKDRIDLERHYNIK"
BASE COUNT          196 a           82 c          100 g          183 t
ORIGIN      
        1 gataatgatc ccttttatga gcaagttcta aatgactttt taacttgcag ggttaaaaca
       61 ttaaaagttt ttcgttctat tgatgatact aaagtcattc ttattgacac tgctcgtgga
      121 cctcttgtac ttaaagttta tgcgccaaag cataaaatga ccgaacgttt cttaaaatct
      181 tgcataaaaa aagattatta tgaaaattta atttatcaaa ccgatcgggt acgtggtgaa
      241 ggcatccaat caattaatga ctattttttg ctagctgaac gtaaaacatt aaatttcgct
      301 cattattata ttatgctcat tgagtatatt gaaggtgtcg ggttaaatga gtatttggaa
      361 atttctgaag atctgaagga tcaattatca gaatcgataa aagagttgca tcagcatgga
      421 atggtgtctg gcgatcctca taagggaaat tttatagtat ccgaaaaagg tttaagacta
      481 attgaccttt caggtaaaaa aacaaccgct gttttaaaag caaaagatcg cattgatctt
      541 gaacggcact ataatataaa a
//
LOCUS       DAZFDC010000452          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFDC010000452 DAZFDC010000000
VERSION     DAZFDC010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.455"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>558
                     /gene="escU"
                     /locus_tag="ACHJ48_005282"
     CDS             <1..>558
                     /gene="escU"
                     /locus_tag="ACHJ48_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO4710583.1"
                     /translation="YFMGHSYANEFANFTGLNAYQALVVVAFFVFLLWKGVLFGYLLF
                     SVFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKK
                     STVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLAR
                     TLYKNIHKGQYITEDFFEPVAQLIR"
BASE COUNT          181 a           95 c          111 g          171 t
ORIGIN      
        1 ttattttatg gggcattcat atgcaaatga gtttgctaat ttcacaggac tgaacgcata
       61 tcaagctctt gtcgttgttg ccttttttgt ttttctttta tggaaaggcg tgctattcgg
      121 atatctactc ttttcagtat ttgatttctg gttccagaag catgagggac tgaagaaaat
      181 gaaaatgagt aaagatgagg tgaaacgaga agccaaggat actgatggta accctgaaat
      241 taaaggggag cgccgtcgcc ttcattccga gatacaaagt ggaagtttgg ctaataacat
      301 caaaaaatca accgttattg ttaaaaaccc gactcacatt gcgatttgcc tatactataa
      361 acttggggag actccattac ctttagttat tgaaacagga aaagatgcca aagctctaca
      421 gatcattaaa ctggctgaac tctatgatat tccagtgatt gaagatattc ctttagcaag
      481 aactctctat aagaatatac ataaaggaca atatataaca gaagactttt ttgaacctgt
      541 ggcacaattg attcgtat
//
LOCUS       DAZFDC010000453          555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFDC010000453 DAZFDC010000000
VERSION     DAZFDC010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.456"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..174
                     /locus_tag="ACHJ48_005283"
     CDS             <1..174
                     /locus_tag="ACHJ48_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO4710584.1"
                     /translation="HFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFF
                     TNFNTIERIENYL"
     gene            171..>555
                     /locus_tag="ACHJ48_005284"
     CDS             171..>555
                     /locus_tag="ACHJ48_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO4710585.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCITKNYLMVINEYPY"
BASE COUNT          185 a          102 c           87 g          181 t
ORIGIN      
        1 cattttgtag cacaggacca cttcggtctt gatgtaactg atatcaaaaa caagacttac
       61 agcaaatata gattttttcg catatggttc tttttacaac ggcataaaga ctttgctttc
      121 aaaccttttt tcaccaattt taacactatc gaaagaatcg aaaattatct atgaaaagca
      181 aactccaaaa aattatatta tgtttatttc ttctatgctg catctataat ttatggacat
      241 tgcgtcctgt acagattttg tatacatact ctgatgctgg caattcagtc tttctggtcg
      301 ttgatcatct gccatggact gacagcgata aaataaattg gtatctgaag catcagaatg
      361 aaattaaaaa tcaacatccc ctaccagaag gtagttggca tacctggtat gtcattgata
      421 tcggtaatgg cttcactgac tataaaaaat atatcgaagg gccgtatgaa gatctctact
      481 gtttcccgac aattaagagc aatgataatt gcatcacgaa aaattactta atggttatta
      541 atgaataccc ttatc
//
LOCUS       DAZFDC010000454          555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFDC010000454 DAZFDC010000000
VERSION     DAZFDC010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.457"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            252..>555
                     /gene="gadE"
                     /locus_tag="ACHJ48_005285"
     CDS             252..>555
                     /gene="gadE"
                     /locus_tag="ACHJ48_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO4710586.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAEKIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEI"
BASE COUNT          198 a           84 c          105 g          168 t
ORIGIN      
        1 ggcagtaatc cgcaaacgtt aactttttgt ttactattta caagctgata acaaccagga
       61 atcttactta ggatcaatat atggagtgcg tgatggataa atctgaagta ttgattagtg
      121 ttaatagacg tattagttca cgaagggcaa agttcttata ggcgtttact atattgaaca
      181 acgattcgga caaggatgta aataatgaaa aggatgacat gttcgaaacg ataacggcta
      241 aggagcaagt tatgattttt ctcatgacga aagattcttt tcttttacag ggcttttggc
      301 agttgaaaga taatcacgaa atgataaaaa tcaattccct gtcagagatc aaaaaagtag
      361 gcaataaacc cttcaaggtt atcattgata cctatcacaa tcatatcctt gatgaagaag
      421 cgattaaatt tctggagaaa ttagatgccg agaaaattat tgttttggca ccttatcaca
      481 tcagtaaact aaaagctaaa gcgcctattt attttgttag ccgcaaagaa agtatcaaaa
      541 atcttcttga gatta
//
LOCUS       DAZFDC010000455          554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFDC010000455 DAZFDC010000000
VERSION     DAZFDC010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.458"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            262..>554
                     /locus_tag="ACHJ48_005286"
     CDS             262..>554
                     /locus_tag="ACHJ48_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710587.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKA"
BASE COUNT          159 a          107 c          173 g          115 t
ORIGIN      
        1 gtcgtggtta tcgggcggca gaggtaaaaa gaataaaaaa atcccgcaga gttgcgggga
       61 cagacaaaga ttaacgttaa ggagttattt ttgtttctgg tgctcgggca aaaaaacatt
      121 aacgcagaga aattattagc accacagtca gtttgtgaaa atgtgaagat attcagaagt
      181 tttattcagt catgatacag gcatcctccg ggatgcctgt tgtttttgtg cgtaacagtt
      241 atcacagtaa agggtgagac aatgggcaaa ggtggcggca aggcgcacac gccggtagag
      301 gcaaaggaca atcttaagtc cacgcagatg atgagcgtga ttgatgccat tggtgaaggg
      361 ccgattgaag gtccggtgaa ggggctgcag agtatcctgg tgaacaaaac cccgctgacg
      421 gacacggacg gtaatcctgt gatacatggt gtgacagcgg tctggcgcgc cggggagcag
      481 gagcagacac cacctgaagg ctttgagtcc tccggggcgg aaaccgcact gggcgtggaa
      541 gtgacgaagg caaa
//
LOCUS       DAZFDC010000456          551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFDC010000456 DAZFDC010000000
VERSION     DAZFDC010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.459"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>551)
                     /locus_tag="ACHJ48_005287"
     CDS             complement(<1..>551)
                     /locus_tag="ACHJ48_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710588.1"
                     /translation="RMTNSFYLFWVNPIRYRITQSWTGYDSSLEGHSRLPDIKSVIAI
                     AEIFSKRDEQLSSRDIFTTSVLALLMCAPSRISEILALPADCEITECDGKGIQRYGLR
                     FFSAKGYEGNIKWIPTLMIPVAKKAISRLKELSSQARLLAAEIQKNYSNSTKGTLKEN
                     IPPDLFWYDREKKIKYSNALCLL"
BASE COUNT          178 a          112 c           80 g          181 t
ORIGIN      
        1 taacaagcaa agcgcattag aatatttgat tttcttctct ctatcatacc aaaagagatc
       61 aggaggtata ttttctttaa gggttccctt cgttgaatta gagtaattct tttgaatttc
      121 agcagccaat aacctcgctt gacttgataa ttcttttaat ctgctaatag cctttttagc
      181 tacaggtatc attaaagttg gaatccattt tatattgcct tcataccctt ttgctgaaaa
      241 aaatcttaaa ccgtatcttt gaatgccctt tccatcacat tctgtgattt cacaatccgc
      301 tggcaaagct aaaatttccg atatcctact cggtgcacac ataagtaaag caagcacaga
      361 tgtagtaaat atatctcttg acgataattg ttcatcccgc tttgaaaaaa tctcagcaat
      421 cgcaataact gatttgatat caggcaatct agaatggcct tccagtgaag aatcataacc
      481 agtccaagac tgagtaatcc tatacctaat tggatttacc caaaataaat aaaatgagtt
      541 agtcatccta t
//
LOCUS       DAZFDC010000457          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFDC010000457 DAZFDC010000000
VERSION     DAZFDC010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.460"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            286..>545
                     /locus_tag="ACHJ48_005288"
     CDS             286..>545
                     /locus_tag="ACHJ48_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710589.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQL"
BASE COUNT          148 a          102 c          152 g          143 t
ORIGIN      
        1 cgtggtttga tggcgtagat gcacgttgtg acatgtagat gataattatt atcattttgc
       61 gggtcctttc cggcgatccg acaggttacg gggcggcgac ctcgcgggtt ttcgctattt
      121 atgaaaattt tccgggatcc atgtccggtt tctcttcaag ttaactatat gaaaaatata
      181 aaaacaggtc ttctgtgaac cggacatgaa caaaaaacag acatgtaaac cggacatgac
      241 cggttttgtt gtgattgtga ggtgagagtt tttgcgaggt gaggagtggc tacgcagact
      301 gaagttgcca ggcatttaag tctgaccgat cgccagcttc gcagattgca gaaattgccg
      361 ggtgccccga tatcgaataa gcgagggcaa ctggatctgg atgcctggcg cgatttttac
      421 atatcgtatc tgaggagaag taaaaacgat gtgcctgatg gcgatagcga agacgactat
      481 gaggagaaat tgcttattgc cagatgggaa ctgacagcag aacaggctgt tacacagcag
      541 ttaaa
//
LOCUS       DAZFDC010000458          539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFDC010000458 DAZFDC010000000
VERSION     DAZFDC010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.461"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>539
                     /gene="espR3"
                     /locus_tag="ACHJ48_005289"
     CDS             <1..>539
                     /gene="espR3"
                     /locus_tag="ACHJ48_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HFO4710590.1"
                     /translation="KTGSITIEKSQGEGTLPIRHKLEFISTNIAELLDKLTKITDARL
                     CKGFSDWASSVKEGASNDLKENVDRALVRMFKCVKLHSNELNLSSLSLGSVPPLPEWI
                     EMLSLVYNELDSIQVPESCKELELDFNNLTEFPQVPDGITLISVNNNLISYIDSFPPK
                     AKKIFICHNKLSEIPALPDT"
BASE COUNT          175 a          101 c          111 g          152 t
ORIGIN      
        1 aagacgggat ctatcacgat agaaaaaagt cagggggagg ggactttacc cattcggcat
       61 aaattagagt ttatatcgac caatattgca gagttgctgg acaagttaac caaaattaca
      121 gatgccaggc tttgcaaagg tttcagtgac tgggctagtt cagtcaaaga aggcgcatcg
      181 aatgacttga aagaaaatgt ggatagagca ttggtgagaa tgtttaaatg tgttaagctt
      241 cacagtaatg aacttaactt atcaagcctt tctttgggtt ctgtgccgcc tcttcctgag
      301 tggattgaaa tgcttagcct tgtttataat gaacttgatt caatacaggt gcccgaatcg
      361 tgcaaagaat tagaactcga tttcaataac cttacagaat ttccacaagt acctgatgga
      421 attaccctga tctccgtaaa taataacctg atatcgtata ttgactcatt tccgccaaag
      481 gctaagaaaa tttttatttg tcacaataag ctatcggaaa taccagcact accagacac
//
LOCUS       DAZFDC010000459          534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFDC010000459 DAZFDC010000000
VERSION     DAZFDC010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.462"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>534
                     /locus_tag="ACHJ48_005290"
     CDS             <1..>534
                     /locus_tag="ACHJ48_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4710591.1"
                     /translation="STGLKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDR
                     IVRALAAGMGVARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITK
                     VENAFMAWVGGANEASGVTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASG
                     AMSATAGLVTAARNEVAL"
BASE COUNT          116 a          119 c          188 g          111 t
ORIGIN      
        1 tcgacagggc tgaaaatctc cggtgccagt acggcagagg cgggctcggt gatcacccag
       61 ttcagtcagg cgctggcgca gggtgtattg cgtggtgagg agtttaattc ggtcaatgaa
      121 agtggtgacc ggatcgtacg tgcactggct gcgggtatgg gcgtggcccg taaagatctg
      181 aaggcaatgg cggatgacgg aaaactgaca gcggataaag tggtccccgc gttaatcagc
      241 cagctgggga tattacgtga tgaatatgcg gccatgccgg aaacggtttc cagtagtatc
      301 acaaaggtgg aaaacgcctt tatggcctgg gtgggcggtg cgaatgaggc cagcggggtg
      361 acaaaaacgc tctccggcat gctgaacggt gttgccggac agattgataa tgtggcaaca
      421 gccgtgggcg cgctggttgc cgtcggggtt gcccggtact ttggcaatat ggcctccgga
      481 gcgatgtctg ccacggcagg acttgtgacg gctgcacgta atgaagttgc actg
//
LOCUS       DAZFDC010000460          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFDC010000460 DAZFDC010000000
VERSION     DAZFDC010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.463"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>530
                     /gene="yjgL"
                     /locus_tag="ACHJ48_005291"
     CDS             <1..>530
                     /gene="yjgL"
                     /locus_tag="ACHJ48_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFO4710592.1"
                     /translation="KQKSEVLNTWRVGMNNFARNAGGQDNTRNILNPKTFLEFLVKIF
                     TLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKFVMNNPEGERADSPKVIIEI
                     SLSTITTMGTRQGHTAIIFPQPDGSTNRYERKSFERKDESSLHLITNKVLACYQREAN
                     KEIARLLNNHQKLNNL"
BASE COUNT          184 a           89 c          108 g          149 t
ORIGIN      
        1 taaacaaaaa agtgaagttt taaatacctg gcgtgttgga atgaataatt ttgcccgtaa
       61 tgccgggggg caggataaca caagaaatat ccttaatcct aagacatttt tggagttttt
      121 ggtaaaaata tttaccctgg gttatgtgga ttttagcaaa cgctccaacg aagcgggaag
      181 aaatatgatg gctcatattg agtcctcatc ttatatcaaa aataatgatg gcagtgagat
      241 aatgaagttt gttatgaata atcctgaagg ggaacgagcg gattcaccca aggtgattat
      301 agaaatttca ctttccacta ttactactat ggggactcgt caaggacata cagccattat
      361 atttccacaa cctgatggtt cgactaaccg ttatgaaaga aagtcctttg aaagaaaaga
      421 tgagagttca ttacacctga ttactaacaa ggttctggcg tgttaccaac gcgaagctaa
      481 caaggaaata gctcgtctat taaataatca tcagaagtta aataatctac
//
LOCUS       DAZFDC010000461          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFDC010000461 DAZFDC010000000
VERSION     DAZFDC010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.464"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..95
                     /locus_tag="ACHJ48_005292"
     CDS             <1..95
                     /locus_tag="ACHJ48_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710593.1"
                     /translation="RSSLKENTETVEMNSKVWGRLKKIITFFKK"
     gene            complement(171..473)
                     /gene="bamE"
                     /locus_tag="ACHJ48_005293"
     CDS             complement(171..473)
                     /gene="bamE"
                     /locus_tag="ACHJ48_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO4710594.1"
                     /translation="MKNETQESVKTKIVKGKTTKQDVLASFGEPDSRSLIDGEEQWSY
                     TMYNSQSKATSFIPVVGLLAGGADSQTKSLTVSFKGEKVSTYIFNAGTSNVKTGIF"
BASE COUNT          145 a          100 c          110 g          166 t
ORIGIN      
        1 taagatcctc tttaaaggaa aatactgaga ctgtagaaat gaacagtaaa gtttggggga
       61 ggctgaaaaa gataatcact ttctttaaaa agtgatttat gtttctgtaa aaagcccgta
      121 ggtatcaact gcgggctaaa ttttcccttg atgctggttt tgtcttttat ctaaaaaatg
      181 ccagtcttca cgttgcttgt tccagcatta aagatgtatg tgctgacttt ttcgcctttg
      241 aaagaaactg tcagagattt agtttgtgag tctgcgccac ctgcaagcag tcccacaacg
      301 gggatgaaag aggttgcttt ggactggctg ttatacatag tgtatgacca ttgttcttca
      361 ccatcgatca aagaacggct gtcaggttca ccgaacgatg ctaacacgtc ctgttttgta
      421 gttttgcctt taacaatttt ggttttcaca ctttcctgcg tctcattttt caatgactgg
      481 ttgcctactg aagaacaacc tgccaaggtc agaaaaacga a
//
LOCUS       DAZFDC010000462          505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFDC010000462 DAZFDC010000000
VERSION     DAZFDC010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.465"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>505
                     /locus_tag="ACHJ48_005294"
     CDS             <1..>505
                     /locus_tag="ACHJ48_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO4710595.1"
                     /translation="LESGIRISIGSGQYSVHYVQLLDGFSVEPVRGGLLDRLLGREHR
                     MDRRAVALERQLNGGVDFLSSVNNYFQSVMAEHRENKTGNKILMEKINSCVFGTDSNH
                     FSCPESFLTCPITLDTPETGVFMRNSRGAEICSLYDKDALVQLVETGGTHPLSREPIT
                     ESMIMRKD"
BASE COUNT          159 a           77 c          126 g          143 t
ORIGIN      
        1 cttgaaagtg gaataagaat atccattggt agtggccaat attctgttca ctatgtccaa
       61 ctactggatg gattttcagt tgaaccggtg agaggaggct tactggatag gctattgggg
      121 cgtgagcatc gaatggatag aagggctgtg gctctggaaa ggcaattaaa tggaggtgtc
      181 gattttttaa gtagtgttaa taactatttt cagagtgtca tggcagaaca cagagaaaat
      241 aaaacaggta ataaaatatt aatggaaaaa ataaattctt gtgtatttgg aacggattct
      301 aatcactttt cttgcccgga gtcatttttg acatgcccga taacgctgga cacacctgag
      361 actggagtgt tcatgagaaa ctcacgaggt gctgagatat gctctctata tgataaggat
      421 gcgttagtgc aacttgttga aactggtgga actcatcctc tgagtcgaga acctataaca
      481 gaatcaatga ttatgagaaa agacg
//
LOCUS       DAZFDC010000463          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFDC010000463 DAZFDC010000000
VERSION     DAZFDC010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.466"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..302
                     /locus_tag="ACHJ48_005295"
     CDS             <1..302
                     /locus_tag="ACHJ48_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO4710596.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>504
                     /locus_tag="ACHJ48_005296"
     CDS             299..>504
                     /locus_tag="ACHJ48_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO4710597.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALW"
BASE COUNT          114 a          117 c          169 g          104 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcagggac
      421 cagcattggc gcattcaatg gtgccattat cgcggcctct cccgatctgt cagaagctgc
      481 cgtacgcctg gaggcgctct ggga
//
LOCUS       DAZFDC010000464          502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFDC010000464 DAZFDC010000000
VERSION     DAZFDC010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.467"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            79..>502
                     /locus_tag="ACHJ48_005297"
     CDS             79..>502
                     /locus_tag="ACHJ48_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710598.1"
                     /translation="MDHTKHSILSSLQDKEDDVDELKYSAEDFDSLTVADLYDIEIAM
                     QDFLNDINFENSKDNKVRFDEDTYDFNINGKRRGMFGKGTRAVMHAIFTICFAEFLSR
                     KGNPFIGFVVLDSPLVTHFDKDRGGSLSDVNSVSLSDSF"
BASE COUNT          175 a           73 c           94 g          160 t
ORIGIN      
        1 taagacgtta gaagataaaa atataaaaca atacattcat ctcttgaatc actttatgaa
       61 tataaagaca atcttttaat ggatcacaca aaacatagca ttctatcttc attacaagat
      121 aaagaagatg atgttgacga acttaaatac tccgccgagg actttgactc cttaacagta
      181 gctgatttgt atgatataga aatagcgatg caagactttt tgaatgatat aaattttgaa
      241 aattctaaag ataacaaagt gcgatttgac gaagatacat atgactttaa tatcaatggg
      301 aaacgccgag gaatgtttgg taaaggtacc cgtgcagtaa tgcatgctat atttaccatt
      361 tgctttgcgg aatttttatc aagaaaaggg aatccattca ttggatttgt tgtgcttgat
      421 agtccattag tgacgcactt tgataaagat agagggggtt ctctatcgga tgttaatagc
      481 gtcagtctca gtgattcatt tt
//
LOCUS       DAZFDC010000465          500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFDC010000465 DAZFDC010000000
VERSION     DAZFDC010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.468"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            33..308
                     /locus_tag="ACHJ48_005298"
     CDS             33..308
                     /locus_tag="ACHJ48_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO4710599.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(388..>500)
                     /locus_tag="ACHJ48_005299"
     CDS             complement(388..>500)
                     /locus_tag="ACHJ48_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFO4710600.1"
                     /translation="YFNFTTLHDYNHFCDFIEFKHKNIIMNTSQYTGSSW"
BASE COUNT          201 a          109 c           78 g          112 t
ORIGIN      
        1 aaaacacttt tcatggagca aaggagaaaa caatgccatt ttcaatcaaa aacagatttt
       61 caagttcaca agtacattac ccggaaatat ccggtcccat aaaagacaag ccagcgtcaa
      121 agaactgcat acttacatca acaacatgta atgtagatag ctatacagtg taccaaaaaa
      181 aagcctgtag ttttgacatg cgcccacccg gcgcaggaga aagaacccca aaactaaaac
      241 tctcagttac tgagatgaca tggctatcta aaactataga aacagagata cacaacacaa
      301 aagaatagca accacttacc agagaacaca aaaagccaca gacccgaaac acctgactgc
      361 aagcaaccac ctccacagga ggtggtttta ccatgaactt cctgtgtact gacttgtgtt
      421 cataataata tttttgtgtt taaactcaat aaagtcacaa aaatgattgt aatcatgcaa
      481 tgtagtaaaa ttaaaatatt
//
LOCUS       DAZFDC010000466          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFDC010000466 DAZFDC010000000
VERSION     DAZFDC010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.469"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>497
                     /gene="wzx"
                     /locus_tag="ACHJ48_005300"
     CDS             <1..>497
                     /gene="wzx"
                     /locus_tag="ACHJ48_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O157 family O-antigen flippase"
                     /protein_id="HFO4710601.1"
                     /translation="TLKKYDTSSALGREQERYRIISLSVISSLISKILSLLSLILTVS
                     LTLPYLGQERFGVWMTITSLGAALTFLDLGIGNALTNRIAHSFACGKNLKMSRQISGG
                     LTLLAGLSFVITAICYITSGMIDWQLVIKGINENVYAELQHSIKVFVIIFGLGIYSNG
                     VQKVY"
BASE COUNT          158 a           68 c           99 g          172 t
ORIGIN      
        1 actttaaaaa aatatgatac atcaagtgct ttaggtagag aacaggaaag gtacaggatt
       61 atatccttgt ctgttatttc aagtttgatt agtaaaatac tctcactact ttctcttata
      121 ttaactgtaa gtttaacttt accttattta ggacaagaga gatttggtgt atggatgact
      181 attaccagtc ttggtgctgc tctgacattt ttggacttag gtataggaaa tgcattaaca
      241 aacaggatcg cacattcatt tgcgtgtggc aaaaatttaa agatgagtcg gcaaattagt
      301 ggtgggctca ctttgctggc tggattatcg tttgtcataa ctgcaatatg ctatattact
      361 tctggcatga ttgattggca actagtaata aaaggtataa acgagaatgt gtatgcagag
      421 ttacaacact caattaaagt ctttgtaatc atatttggac ttggaattta ttcaaatggt
      481 gtgcaaaaag tttatat
//
LOCUS       DAZFDC010000467          496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFDC010000467 DAZFDC010000000
VERSION     DAZFDC010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.470"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>496)
                     /locus_tag="ACHJ48_005301"
     CDS             complement(<1..>496)
                     /locus_tag="ACHJ48_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO4710602.1"
                     /translation="GTPEGERYQPCVNISAEPEAYFRIAPEDWLQAEMQGEIVALVHS
                     HPGGLPWLSEADRRLQIKSALPWWLVCRGEIHKFRCVPHLTGRRFEHGVTDCYTLFRD
                     AYHLAGITLPDFEREDDWWRNGQNLYLDNMAATGFYRVPLSSAQAGDILLCCFGASVA
                     NHAA"
BASE COUNT          106 a          170 c          135 g           85 t
ORIGIN      
        1 atggcggcat gattggccac cgatgcgcca aagcagcaca gcaggatatc gcccgcctgt
       61 gcagaggaca ggggcacccg gtaaaaacca gtcgccgcca tattgtccag gtaaaggttc
      121 tgaccgttgc gccaccagtc atcctcacgc tcaaaatccg gcagcgttat ccccgccaga
      181 tggtatgcat cccggaacag ggtgtaacag tccgtcaccc cgtgctcaaa acgacgcccg
      241 gtcaggtgcg gcacacagcg gaatttatga atttcccccc ggcagaccag ccaccagggc
      301 agggcactct ttatctgcag ccgccggtcg gcctcgctca gccagggcag accaccaggg
      361 tggctgtgaa ccagcgccac aatctccccc tgcatctctg cctgcagcca gtcttccggc
      421 gcaatacgaa aatacgcctc cggctctgcg gagatattca cgcagggctg gtaccgttcg
      481 ccctccgggg tgccta
//
LOCUS       DAZFDC010000468          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFDC010000468 DAZFDC010000000
VERSION     DAZFDC010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.471"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>494
                     /locus_tag="ACHJ48_005302"
     CDS             <1..>494
                     /locus_tag="ACHJ48_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO4710603.1"
                     /translation="EPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYG
                     WDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDG
                     GSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVAQMQSQMDPVYTPARKIH
                     LYHC"
BASE COUNT          106 a          143 c          170 g           75 t
ORIGIN      
        1 gagccgctgg tcgaaagccg ctatctttac gacccgctgg gccgcagggt ggcaaaacgg
       61 gtgtggcgac gtgaacggga cctgacgggc tggatgtcgc tgtcacggaa accgcaagtg
      121 acctggtacg gctgggacgg cgaccggctg accacaatac agaacgacag aacccgcatc
      181 cagacgattt atcagccggg gagcttcacg ccactcatca gggttgaaac cgccaccggt
      241 gagctggcga aaacgcagcg ccgcagcctg gcggatgcgc ttcagcagtc cggcggcgaa
      301 gacggtggca gtgtggtgtt cccgccggtg ctggtgcaga tgctcgaccg gctggaaagt
      361 gaaatcctgg ctgaccgggt gagtgaggaa agccgccgct ggctggcatc gtgcggcctg
      421 actgtggcgc agatgcaaag ccagatggac ccggtataca cgccggcgcg aaaaatccac
      481 ctgtaccact gcga
//
LOCUS       DAZFDC010000469          491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFDC010000469 DAZFDC010000000
VERSION     DAZFDC010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.472"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..397)
                     /locus_tag="ACHJ48_005303"
     CDS             complement(<1..397)
                     /locus_tag="ACHJ48_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710604.1"
                     /translation="MPNTSIHLSRCNILQNNKLQPTEIYKESQQTAKLEFFCDEFLKI
                     SQSRYGLSTSTDSIANLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKN
                     EFAADYEPYTLTFTRGENNEGILSVESKEG"
BASE COUNT          146 a           77 c           86 g          182 t
ORIGIN      
        1 acccttcttt gctttcgaca ctcaaaatac cttcgttgtt ttcacctcgg gtaaaggtaa
       61 gggtataggg ttcataatcc gctgcaaatt catttttata cgttatgaca tgttcgacaa
      121 aatgtcttat tggtacgaaa ccataactgt gaacgtcaat tttttgggtc ttgatactat
      181 caattgcatc tgaggctttc gtgaaaaaag ccaataaatt agctatgctg tctgttgagg
      241 ttgataaccc gtatctactt tgagatattt ttagaaactc atcgcaaaaa aactcaagtt
      301 tggcggtttg ttgtgattct ttgtaaattt ctgttggttg caatttatta ttttgtaaaa
      361 tattacagcg agataagtga atagatgtat ttggcataaa tgattccttg tattgtgagt
      421 taacaagata caatttaatc tttgcagaat atattcacta tcaaatttag ctcattttat
      481 tacgaagacc t
//
LOCUS       DAZFDC010000470          491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFDC010000470 DAZFDC010000000
VERSION     DAZFDC010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.473"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(10..156)
                     /locus_tag="ACHJ48_005304"
     CDS             complement(10..156)
                     /locus_tag="ACHJ48_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710605.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
BASE COUNT          147 a          107 c           97 g          140 t
ORIGIN      
        1 atttagtttc taaaatggaa tttttaacgt tttatacttc acattacaat ctgtaccata
       61 agcctcctca gcgaggttgc ctgaataccc tgtcgtgccc atttttccat cggacgcagt
      121 tgtctgctct ctcgaactac tggcaataaa aatcaaccag acagcttgat gtccgttaca
      181 tgcaggacta atcacgattg gttaccgttt acaaaacttg cgtaacattg gaggagaacg
      241 aataatattc tctagctggt acgcgttcca tagcgaaacg ggcaaggtcc aacagcaaga
      301 gctgaaactc agggggattt atcagtcgat ttattcgcca tggatgaatc acgctgtacg
      361 gttatggtag ctcttagcga caaggcataa tcatcagtac accaatgtaa caaccgttga
      421 aaaagactca ttattttatg aaccagccat cccatttcca gagtggtaaa tatgatttgt
      481 taaagtgtaa c
//
LOCUS       DAZFDC010000471          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFDC010000471 DAZFDC010000000
VERSION     DAZFDC010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.474"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..150
                     /locus_tag="ACHJ48_005305"
     CDS             <1..150
                     /locus_tag="ACHJ48_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4710606.1"
                     /translation="VNGMDSIKLLDKVIIYQQQNEQLYPAQEHLLLQLCMRVTKKLTD
                     ILMPP"
     gene            180..464
                     /locus_tag="ACHJ48_005306"
     CDS             180..464
                     /locus_tag="ACHJ48_005306"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO4710607.1"
                     /translation="MLLALLSSTDNFCLSSTELSERLDVSRTYITRACDSLEKFGFIK
                     RMESKEDRRSKNIYLTSDGNLYLQRTTRIYGRYLKKYGATLQMMKSKHLK"
BASE COUNT          173 a           75 c           85 g          151 t
ORIGIN      
        1 gttaatggaa tggatagtat taaattactt gataaagtta ttatctatca acaacagaat
       61 gagcaattat atccagcaca ggaacatctt cttttacaac tttgtatgcg agttaccaaa
      121 aaacttactg atatattaat gcctccctga agggggatgg tataaacgat acaacgctta
      181 tgcttctagc cttgttatcc agtactgata atttctgctt atcttcgact gaactcagtg
      241 aaagattaga tgtttccaga acatatatca ccagagcttg tgattctctg gaaaaatttg
      301 gttttattaa acgtatggaa agtaaggaag atcgacgtag taagaatatt tatctgactt
      361 cagacggtaa tttgtattta cagcggacca caaggatata tggacgctat ttaaaaaaat
      421 atggagcaac cttacagatg atgaaatcaa aacatttaaa ataatcagca aaaaggtttt
      481 atct
//
LOCUS       DAZFDC010000472          475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFDC010000472 DAZFDC010000000
VERSION     DAZFDC010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.475"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..233)
                     /locus_tag="ACHJ48_005307"
     CDS             complement(<1..233)
                     /locus_tag="ACHJ48_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710608.1"
                     /translation="MNVRNVKFDNLSFYEEKIRWRMRRQISNAEAIFYENQEEHIRRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSMFG"
     gene            complement(220..>475)
                     /locus_tag="ACHJ48_005308"
     CDS             complement(220..>475)
                     /locus_tag="ACHJ48_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710609.1"
                     /translation="ILTAELYVDEFTAGRERKRFEFTGVEHLSQVMDIAELIDNQNAG
                     NIKDATIVYKAKKYKISLHLFGGFLSFTFKTMRVIDNECA"
BASE COUNT          144 a          113 c           65 g          153 t
ORIGIN      
        1 tcaccaaaca tactcatcac ttccagaccg tttacagatg tccatctttg ttcactttcc
       61 caataaacaa gaaccggttt gcgacaggta tatctgccta aaaagcgccg aatgtgttct
      121 tcctgattct cgtaaaatat tgcctctgca ttcgagattt gccgacgcat tcgccagcgg
      181 attttttctt cataaaaaga caagttgtcg aatttgacat tacgcacatt cattgtcaat
      241 aactcgcatg gttttaaatg taaaacttaa aaaaccacca aacaaatgaa gtgaaatttt
      301 atacttcttt gccttataaa caattgtcgc atctttaata ttacctgcat tttggttatc
      361 tataagttca gcaatatcca taacctgcga taaatgttcc actcctgtaa actcgaacct
      421 ttttcgttcc cttccggctg taaattcatc aacataaagc tccgccgtca atata
//
LOCUS       DAZFDC010000473          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFDC010000473 DAZFDC010000000
VERSION     DAZFDC010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.476"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(126..>473)
                     /locus_tag="ACHJ48_005309"
     CDS             complement(126..>473)
                     /locus_tag="ACHJ48_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HFO4710610.1"
                     /translation="PAFMLVPVALETVANQTIKSASVKGADANAGVINPIQNFAEVIA
                     EARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVA
                     PLDWRGLVRSSVA"
BASE COUNT           98 a          140 c          120 g          115 t
ORIGIN      
        1 tcaatggtgg taccgtcctg tacaaaattt ttcgccataa tgctgtttct ccggacagcc
       61 cctgtggggc tgtttttcag gcataaaaaa agccctttcg ggcagtgatt gtgataacgc
      121 ggttatcagg ccaccgacga acgcaccagc ccgcgccagt caagtggtgc cactccggca
      181 tcaatacgga tttttgtggc aatgccgtca gtggtgaaac cttcctgctg atcaatgtat
      241 ggcgtgtcca caccatccag ccaggccact tcaatggtgt cagtgccctg tgccgccgcc
      301 agataccagg ttttcgggtc tgccgcatca agacgcgctt ctgcaatcac ctcagcaaag
      361 ttctggatag ggttaatgac accggcgttt gcatccgccc ctttcacact ggccgatttg
      421 atggtctggt tcgccaccgt ctccagtgcc accggtacca gcataaaggc cgg
//
LOCUS       DAZFDC010000474          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFDC010000474 DAZFDC010000000
VERSION     DAZFDC010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.477"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(126..>473)
                     /locus_tag="ACHJ48_005310"
     CDS             complement(126..>473)
                     /locus_tag="ACHJ48_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HFO4710611.1"
                     /translation="PAFMLVPVALETVANQTIKSASVKGADANAGVINPIQNFAEVIA
                     EARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVA
                     PLDWRGLVRSSVA"
BASE COUNT           95 a          141 c          123 g          114 t
ORIGIN      
        1 tcaatggtgg tgccgtcctg tacaaaattt ttcgccataa tgctgtttct ccggacagcc
       61 cctgtggggc tgtttttcag gcataaaaaa agccctttcg ggcagtgatt gtgataacgc
      121 ggttatcagg ccaccgacga acgcaccagt ccgcgccagt caagcggtgc cacaccggca
      181 tcaatacgga tttttgtggc aatgccgtca gtggtgaaac cttcctgctg gtcaatgtat
      241 ggcgtgtcca ccccatccag ccaggccact tcaatggtgt cggtgccctg tgccgccgcc
      301 agataccagg ttttcggatc tgccgcatca agacgcgctt ctgcaatcac ctcagcaaag
      361 ttctggatgg ggttaatgac accggcgttt gcatccgccc ctttcacact ggccgatttg
      421 atggtctggt tcgccaccgt ctccagtgcc accggtacca gcataaaggc cgg
//
LOCUS       DAZFDC010000475          470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFDC010000475 DAZFDC010000000
VERSION     DAZFDC010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.478"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            155..415
                     /gene="iraP"
                     /locus_tag="ACHJ48_005311"
     CDS             155..415
                     /gene="iraP"
                     /locus_tag="ACHJ48_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO4710612.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          158 a           86 c          102 g          124 t
ORIGIN      
        1 taattattta aataagagaa acgtttcgct ggtaatcaaa caaaaaatat ttgcgcaaag
       61 tatttccttt gccataaaaa taatacctcc agacactatg aagttgtgaa acataatgtt
      121 aacttctcca tactttggat aaggaaatac agacatgaaa aatctcattg ctgagttgtt
      181 atttaagctt gcccaaaaag aagaagagtc gaaagaactg tgtgcgcagg tagaagcttt
      241 ggagattatc gtcactgcaa tgcttcgcaa tatggcgcaa aatgaccaac agcggttgat
      301 tgatcaggta gagggggcgc tgtacgaggt aaagcccgat gccagcattc ctgacgacga
      361 tacggagctg ctgcgcgatt acgtaaagaa gttattgaag catcctcgtc agtaaaaagt
      421 taatcttttc aacagctgtc ataaagttgt cacggccgag acttatagtc
//
LOCUS       DAZFDC010000476          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFDC010000476 DAZFDC010000000
VERSION     DAZFDC010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.479"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>469
                     /locus_tag="ACHJ48_005312"
     CDS             <1..>469
                     /locus_tag="ACHJ48_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO4710613.1"
                     /translation="KFPLKELNNPEHDSYAISEKSHGREEIRLHIVCEVPDELIDFTF
                     EWKGLKKLCVAVSFRSIIAEQKKEPKMTVRYYISSADLTAGKFATAIRNHWHVENKLH
                     WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMD"
BASE COUNT          164 a           81 c          114 g          110 t
ORIGIN      
        1 aaaatttccg ctgaaagaat taaataatcc agagcatgac agttacgcaa ttagtgaaaa
       61 gagtcacggc agagaagaaa tccgtcttca tattgtttgc gaagtccctg atgaacttat
      121 tgatttcacg tttgaatgga aagggctgaa gaaattatgc gtggcagtct cctttcggtc
      181 cataatagca gaacaaaaga aagagccaaa aatgacggtc agatattata tcagttctgc
      241 tgatttaacc gcagggaagt tcgccacagc aatccgaaac cactggcacg tggagaataa
      301 gctgcactgg cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa
      361 tgcagcagaa ttattttcag ggatacggca cattgctatt aatattttga cgaatgataa
      421 ggtattcaag gcagggttaa gacgtaagat gcgaaaagca gccatggac
//
LOCUS       DAZFDC010000477          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFDC010000477 DAZFDC010000000
VERSION     DAZFDC010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.480"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            108..254
                     /locus_tag="ACHJ48_005313"
                     /pseudo
     CDS             108..254
                     /locus_tag="ACHJ48_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(279..>467)
                     /locus_tag="ACHJ48_005314"
     CDS             complement(279..>467)
                     /locus_tag="ACHJ48_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710614.1"
                     /translation="TGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILF
                     TTIMLSAIPSAKMSLSAH"
BASE COUNT          136 a           97 c           95 g          139 t
ORIGIN      
        1 gtacacttca cattttttca ttacatctct gaagagaacg tcgttaatga ttggtcttgc
       61 tcattgttaa taacaaaata ttgaatttac cgattaggaa actttttcct acgccgattg
      121 cctccagcta tggcatgaac tttgagttta tgccagaact gaagtggagt ttcggctacc
      181 caggcgcgat tatctttatg atcctcgcgg gcctggcacc gtatctgtac tttaagcgca
      241 agaactggct gtaactatac gatgtcgctg tgagtaagtt agtgtgctga cagcgacatt
      301 ttagcagagg gaatcgcaga taacataatt gttgtgaata atatccatgc cacgttgagt
      361 tggacgctta atgactcaca tctcacccct tcaattgctg cagagataat agaaaaaagt
      421 gtcaaaccca atgaaatcaa tacaaagtag attgccttac atccagt
//
LOCUS       DAZFDC010000478          465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFDC010000478 DAZFDC010000000
VERSION     DAZFDC010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.481"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..449)
                     /locus_tag="ACHJ48_005315"
     CDS             complement(<1..449)
                     /locus_tag="ACHJ48_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO4710615.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFASGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDV"
BASE COUNT          104 a          142 c          118 g          101 t
ORIGIN      
        1 acatcaccgg cactgtaacc atattcacgc atcgaggcag ccgaacgggc aaacagggcc
       61 gcattatccg aaaatgccgt gcctgtccgc tggctgatat ccatcagcac tttctgtgat
      121 gacgaaaatt catcggatga ctgtgatgcc tgtttcagac gggcatttac ggaactccac
      181 tcatccgcca gtgaaatcag gtgtccggag gcaaaggcac cggcaaatgc gccagccatt
      241 ccgacagcag aaccgcgaat ttccgtcaac tggctgttca gttctgccag ggcacgtcgc
      301 tgctcccggg ctgccgcagc ggcctgacgc ccgccattct gcagggtccg gtaatattca
      361 ctgcccatac gggacgcccg ctggatctcc gactggaatg actgtgaatt tgccgaaatt
      421 ttgataatca gttcacgtaa cgtcgccatt cacctttctc cgggc
//
LOCUS       DAZFDC010000479          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFDC010000479 DAZFDC010000000
VERSION     DAZFDC010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.482"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>461)
                     /locus_tag="ACHJ48_005316"
     CDS             complement(<1..>461)
                     /locus_tag="ACHJ48_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO4710616.1"
                     /translation="EITATLVKPVPDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPHE
                     YTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFSAASGNRKPVVKIASS
                     AGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKAAEEGRTL"
BASE COUNT           91 a          137 c          127 g          106 t
ORIGIN      
        1 agcgtgcgcc cttcctctgc ggctttggtc atgacctcct caagggaggc tgccagcgct
       61 gcacgcttgt tttcaaaact tttaatctgt tcgccaatat tcattatggt cttttcctta
      121 tgaaaaacgg ttgttgactg tgccgcagcg ccggcagaag atgcgatttt caccaccggt
      181 ttccggttgc cggacgcggc agaaaacggg cggtcgtaag atttaatggt ccggatggtg
      241 cattccgcat tcgcgggcac ggtgacggca gacacctcca tcagttccca gcgcagaaaa
      301 tgcagtccgc ctccgtccag aaaggtgtat tcatggggac ggaagcccac ggacagcccc
      361 ctgaccagcc cggtcttaat ggccgcccag acctcatcca gccgggcagc cagttgcgac
      421 ggcatatccg gtacgggctt caccagtgtt gccgtgattt c
//
LOCUS       DAZFDC010000480          460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFDC010000480 DAZFDC010000000
VERSION     DAZFDC010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.483"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(263..>460)
                     /locus_tag="ACHJ48_005317"
     CDS             complement(263..>460)
                     /locus_tag="ACHJ48_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO4710617.1"
                     /translation="TTRNIRFPNQMIEQINIALDQKGSENFSAWVIESCRRELAADIK
                     YARQLTIKKNDTQYALRWLFI"
BASE COUNT          124 a           85 c          103 g          148 t
ORIGIN      
        1 acgatacaca ccggggttta ccgcgtaaac ggagcgcggc gttctcccct catggtcgcc
       61 cgtccggtgc gatggtggaa gaaactggaa tctgttcaat aaaaaaactg ccgtgttgga
      121 gtcacagcag taatgtactg attgggtaga agattattat tgttatgctt tatttttatt
      181 ctatatggct gattatttca attcggaatt aatacagcta atgtctgtga ttttttataa
      241 attcagcaat ataaagaaat agttatatga acagccatcg cagagcatac tgtgtatcat
      301 tcttttttat agtcaactga cgggcatatt ttatgtctgc tgccagctcc cggcggcaag
      361 attcaatgac ccacgcagaa aaattttctg aacctttctg gtcaagagcg atgttaattt
      421 gttcaatcat ctggtttgga aatcggatgt tgcgggttgt
//
LOCUS       DAZFDC010000481          460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFDC010000481 DAZFDC010000000
VERSION     DAZFDC010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.484"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(245..>460)
                     /locus_tag="ACHJ48_005318"
     CDS             complement(245..>460)
                     /locus_tag="ACHJ48_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO4710618.1"
                     /translation="PIADKSSTKNCILDSTTCNVDGFTVFNRRSCSFDMRPPGSADRT
                     PQLRLSISEVAWMSKIIETETNNTNKS"
BASE COUNT          134 a           83 c           91 g          152 t
ORIGIN      
        1 tttcgtgtct ttaagtgata ccagatggta ttgcgctaaa tgtaccaatg ggtataccaa
       61 gagcactaaa aacaaagggg caaccacttt gtaacccctt gattatttgc agaagcgcag
      121 aaaatcgaac tctggaaccc tttcggggca tcctttttca agaatgaaga aaaaaataac
      181 taaaaaatca agttgttagg aagttattca tggctggtgt accaactctt ttctgggcta
      241 atagctatga tttgtttgta ttatttgtct ctgtctctat tatttttgac atccatgcga
      301 cttctgatat tgataacctc agttgtggag ttctgtctgc gcttccggga ggacgcatat
      361 caaaactaca ggatcttctg ttaaacactg taaagccatc tacattgcat gttgttgagt
      421 ctaagataca atttttagta gatgatttat ctgctatagg
//
LOCUS       DAZFDC010000482          456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFDC010000482 DAZFDC010000000
VERSION     DAZFDC010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.485"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            319..438
                     /locus_tag="ACHJ48_005319"
                     /pseudo
     CDS             319..438
                     /locus_tag="ACHJ48_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          124 a          117 c           80 g          135 t
ORIGIN      
        1 aaaaaacagc ttcaggtaat gaggaatatc tcaattttta aacataaaat gcgaattatt
       61 tagtacaaaa agtgaacttt tgatgatctt tccctaatct cacataggat cagatctcgt
      121 ctgatcgttc cccctgcgct cactaccatg gctctttacc acagcagccg gggttgtttg
      181 ggaccgactt tcgacagcag atcccggtgg gcctgccacc atctcttcac aagcttatgc
      241 ttattacatg caaaacgtcc cgttttgcat ctttgtgcct acggcacacc gatgatcttt
      301 ccctgtgatc tttccctgaa tctgcccctg agaacctgaa tcaaacgtct gactcgcaga
      361 accatctgtt tctcgcgctc tgtagaactt catgaaaaag tcatcggtgc ttttatcgaa
      421 aaatacatgt tctactaatt gaagtcatca tcaaaa
//
LOCUS       DAZFDC010000483          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFDC010000483 DAZFDC010000000
VERSION     DAZFDC010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.486"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..248)
                     /locus_tag="ACHJ48_005320"
     CDS             complement(<1..248)
                     /locus_tag="ACHJ48_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO4710619.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYT"
     gene            complement(220..>453)
                     /locus_tag="ACHJ48_005321"
     CDS             complement(220..>453)
                     /locus_tag="ACHJ48_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO4710620.1"
                     /translation="GKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLD
                     HLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
BASE COUNT          121 a          105 c          102 g          125 t
ORIGIN      
        1 gtatagccaa cacgggcaga tttaatcaga ttaacagtgc ggatctgatc attccccata
       61 ctgttcatga tggcgatctg gaatggcagc gttttccatt cgccctcacc atatgaagat
      121 tctttaggca gataataatt ttgatcagcc cattcaactg gcgtcaccgg caatgccctt
      181 atcaggggct gtaatgcagt tgtgacagcg ctcatcatat tattcagttg ttgctctgat
      241 atattcatca agtaaatccg gtaatttatc ccctgcccgc gcacactgat ttgccccctt
      301 agcaataagg gttttcagat ggtcaagatg gcgcggtgtt aaatcaggaa actgtcgctg
      361 catggataaa gggatggaat caagcgtact ggataacgcc attgccagct tactgagggc
      421 aaaaatacag aacccggtgt caatcagttt tcc
//
LOCUS       DAZFDC010000484          452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFDC010000484 DAZFDC010000000
VERSION     DAZFDC010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.487"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>452
                     /gene="espJ"
                     /locus_tag="ACHJ48_005322"
     CDS             <1..>452
                     /gene="espJ"
                     /locus_tag="ACHJ48_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     ADP-ribosyltransferase EspJ"
                     /protein_id="HFO4710621.1"
                     /translation="QEKNITERVLSIFKIKRDFVAVRIQNNQFTDLKNKKIQGHQNTV
                     ASVMDWYNPQKNALGITMGTPRKSADIAKEEHRNALNFMIMEKNTFHEKILNSNDNLQ
                     KSYSKTEDSSWVAASVGSLLDKGAKVYPDTSCSLRLGEPFIFTLPESV"
BASE COUNT          184 a           81 c           78 g          109 t
ORIGIN      
        1 caagaaaaaa acattacaga aagagtttta tccatattta aaataaaacg agattttgtt
       61 gctgtaagaa tccaaaacaa tcagtttact gatttgaaaa acaaaaaaat tcaaggccat
      121 caaaacacag ttgccagtgt catggattgg tataatcccc aaaaaaatgc tttgggtata
      181 actatgggta caccaagaaa atcggccgat atagcaaaag aagagcacag aaatgcacta
      241 aatttcatga taatggaaaa aaacacattt catgaaaaaa tcttaaacag caatgacaat
      301 ttgcaaaaat catacagcaa aacagaagac agtagttggg tcgcagcttc tgtcggctct
      361 cttctagata aaggagcaaa agtatatccc gatacatcat gctctctgag actgggagaa
      421 ccattcatct ttacgctgcc agaatcagtt ag
//
LOCUS       DAZFDC010000485          451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFDC010000485 DAZFDC010000000
VERSION     DAZFDC010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.488"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>451)
                     /locus_tag="ACHJ48_005323"
     CDS             complement(<1..>451)
                     /locus_tag="ACHJ48_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFO4710622.1"
                     /translation="GDLTGVNQVSFRRLRETSSTRNQLNKILRQEIHDKISGVELSDI
                     NKEGFLYQSIGKIRLLALSSALFDIQCPDYIFSRLYRETLIREIGYQNVKQLSFYWQG
                     GQCKPEYGEERFCAELIKYGAGNLEWLFADNPLWTIVKYLLPKSGEI"
BASE COUNT          143 a           99 c           59 g          150 t
ORIGIN      
        1 tttatttctc cacttttcgg caaaagatat ttgactatgg tccataaagg attatcagca
       61 aatagccact ccaaatttcc tgcaccgtat tttattaact cagcacaaaa ccgttcttca
      121 ccatattctg gtttacattg ccctccctgc caatagaatg ataactgctt cacattttga
      181 tatcctatct ctctaatcag agtttctcga tataatcgtg agaaaatata atcagggcat
      241 tgaatgtcaa ataatgcact acttagagca agcaatctta ttttcccaat tgactgatat
      301 aaaaaacctt ccttatttat gtcagataat tccacaccag aaattttatc atgtatttcc
      361 tgacgcaata tcttattgag ttgattacga gtagaactag tttctcttaa acgccgaaaa
      421 ctgacctgat tgacaccggt taaatcacct a
//
LOCUS       DAZFDC010000486          450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFDC010000486 DAZFDC010000000
VERSION     DAZFDC010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.489"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>450
                     /locus_tag="ACHJ48_005324"
     CDS             <1..>450
                     /locus_tag="ACHJ48_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO4710623.1"
                     /translation="GRDRETREWLGWGHAWAHETAVVRRKSEASRFQDFVACGDMTIV
                     RRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQILDSLAEAGIPDESVVGISQGWK
                     LGGAIKTTERKLAEGVLIHGDQPLMAWCVGNARVEPKGNAILITKQA"
BASE COUNT           93 a          101 c          166 g           90 t
ORIGIN      
        1 tggccgtgac agggaaaccc gcgaatggct gggctggggc catgcctggg cgcatgaaac
       61 cgcggtggtc agacggaaga gtgaggcatc ccgttttcag gattttgtgg cctgtggaga
      121 catgacgatt gtccgtcggg tcggggatga cacggcggaa gtggcggagt atgtgcgtcg
      181 tattcatgag gctgagttac tggagcatat cggtattgac ccgtcaggtg tggggcagat
      241 tctggattca ctggcggaag ccgggatccc cgatgagagt gtggtgggga taagccaggg
      301 ctggaagctg ggcggggcca tcaaaaccac cgagcgcaaa ctggctgaag gggtgctgat
      361 tcacggtgat cagcccctga tggcctggtg tgtcggtaat gcccgggtgg agcctaaagg
      421 taacgccatt cttatcacca aacaggccag
//
LOCUS       DAZFDC010000487          449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFDC010000487 DAZFDC010000000
VERSION     DAZFDC010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.490"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(194..270)
                     /locus_tag="ACHJ48_005325"
     tRNA            complement(194..270)
                     /locus_tag="ACHJ48_005325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Arg,seq:tct)
     gene            complement(371..446)
                     /locus_tag="ACHJ48_005326"
     tRNA            complement(371..446)
                     /locus_tag="ACHJ48_005326"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(411..413),aa:Ile,seq:cat)
BASE COUNT          102 a          122 c          128 g           97 t
ORIGIN      
        1 tttctttttg aagattccaa acatcgtgac gttcctttgg gtgggtgagc caacgcccgg
       61 gagcgatctg cccacagaga aagtcacact gaccactccg taagctcacc cccgaaaggc
      121 tctgtggttg gtatgcgccg ggcgtggcgc agatacaaaa aaggcccgcc gaagcgagcc
      181 tggaaaataa gtgtggcgcg ttgtagtgga gtcgaaccac tgaccgattg cttagaaggc
      241 aattgctctg tccggctgag ctaacaacgc agaataccga taatggaccg ccatcgggga
      301 cccggccccg caccaacaac cctgttatgg tgtcgtctgc tcttcctgat aagctaatgg
      361 cggtttgtga tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct
      421 cgctctcacc actgagctaa agggccgag
//
LOCUS       DAZFDC010000488          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFDC010000488 DAZFDC010000000
VERSION     DAZFDC010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.491"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..203)
                     /locus_tag="ACHJ48_005327"
     CDS             complement(<1..203)
                     /locus_tag="ACHJ48_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4710624.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFP"
     gene            complement(355..>437)
                     /locus_tag="ACHJ48_005328"
     CDS             complement(355..>437)
                     /locus_tag="ACHJ48_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO4710625.1"
                     /translation="IDLAYEGSGSGDWRSDAFIVGIGYRF"
BASE COUNT          118 a          123 c           88 g          108 t
ORIGIN      
        1 ggaaatccct ccaccaacag aatgacgctg tactgaccgt actcaacatc catgctgtaa
       61 cgtccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt gctgctggtt
      121 cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc atctttaagc
      181 acgccagaaa tttttactgt catacttttc caccaataaa aaaagcccgc agcagtgacg
      241 ccacgggctt caggacagtg taactttacg tttcctcaaa cgcagttcac cccataaggt
      301 ggatgaacct gcgtatcata acaatattta cagaagataa atcggcgtct gttgtcagaa
      361 acggtatccg ataccaacaa taaatgcatc cgatcgccag tcgccactac cggaaccttc
      421 ataagcaagg tcaatgg
//
LOCUS       DAZFDC010000489          422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFDC010000489 DAZFDC010000000
VERSION     DAZFDC010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.492"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>422
                     /locus_tag="ACHJ48_005329"
     CDS             <1..>422
                     /locus_tag="ACHJ48_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4710626.1"
                     /translation="RAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARSAA
                     QNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTID
                     EIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASR"
BASE COUNT           98 a          111 c          147 g           66 t
ORIGIN      
        1 caagggcagc cgtgtaccgt gctcagcagg ccgtggcggc agcccgcggg acggagatgc
       61 agattgctgc agaggcccgt ctggcggcca cacaggaacg cctgaacaga aatattgctg
      121 ccagaagcgc cgcccagaat gcgctgaaca gtacaacggc ggtgggctca cgtctgatga
      181 gcggtgcgct ggggctggtt ggtggcgtac ccggactggt gatgctgggg gctgcagcat
      241 ggtacacgct gtaccagaat caggagcagg ccagggagtc tgcgcgccag tatgcactga
      301 cgatagatga aatcgcgcat aaaacgccgt caatgtcttt gcctgaagcc tcagataatg
      361 aaggacgaac acgggcggcg ctgacagagc agaaccggct gattgatgaa caggccagtc
      421 gg
//
LOCUS       DAZFDC010000490          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFDC010000490 DAZFDC010000000
VERSION     DAZFDC010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.493"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>413
                     /locus_tag="ACHJ48_005330"
     CDS             <1..>413
                     /locus_tag="ACHJ48_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO4710627.1"
                     /translation="LKGGWNLYTYPLSPVNGMDPLGLYEFKSKNIDDIGIFALAMCNG
                     ESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYS
                     DDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKK"
BASE COUNT          161 a           48 c           91 g          113 t
ORIGIN      
        1 ctgaaggggg gatggaacct gtatacatat ccgctgagcc cggtgaatgg catggatcca
       61 ttaggattat atgaatttaa atcaaaaaat atagatgata ttggaatatt tgcattggca
      121 atgtgtaatg gagaatcaat taacgagaat aaagaatatg gtggactaat atgtaagaag
      181 caaggtgaat atttccccat gaatccgata agttcaaatg ataatgatag tgtagacttg
      241 cgaaatataa aatgccctga aggttcagag agagtaggcg attatcacac tcacggtttt
      301 tactctgacg ataaaggaaa taaagtaaca aaagaaaatg atgtttatga tagtctaaat
      361 ttttcaagca aagatttaac gaattcttat atgaatggaa tgggaaaaaa aga
//
LOCUS       DAZFDC010000491          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFDC010000491 DAZFDC010000000
VERSION     DAZFDC010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.494"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>412
                     /locus_tag="ACHJ48_005331"
     CDS             <1..>412
                     /locus_tag="ACHJ48_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4710628.1"
                     /translation="GETVRYRYDDAYSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLISVKDAQGHETRYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           97 a          123 c          133 g           59 t
ORIGIN      
        1 gcggggagac agtacgctac cgctacgatg atgcgtacag tgagttaccg gcgacgacaa
       61 cagatgcgac gggcagcacc cggcagatga cctggagccg ctacggtcag ttgctggcgt
      121 tcaccgactg ctcgggctac cagacccgct atgaatacga ccgcttcggc cagatgacgg
      181 cggtccaccg cgaggaaggc atcagccttt accgccgcta tgacaaccgt ggccggttaa
      241 tctcggtgaa agacgcacag ggccatgaaa cgcggtatga gtacaacgcc gcaggcgacc
      301 tgactgccgt tatcaccccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
      361 aggcggtcag caccacgcag ggcgggctga cgcgcagtat ggaatacgac ct
//
LOCUS       DAZFDC010000492          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFDC010000492 DAZFDC010000000
VERSION     DAZFDC010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.495"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>412
                     /locus_tag="ACHJ48_005332"
     CDS             <1..>412
                     /locus_tag="ACHJ48_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4710629.1"
                     /translation="GDVIRYAYDNPHSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGHETRYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDL"
BASE COUNT           97 a          117 c          131 g           67 t
ORIGIN      
        1 gtggggacgt catccggtat gcttatgata atccgcacag tgaattaccg gccacgacaa
       61 cagatgcgac gggcagcacc cggcagatga cctggagccg ctacgggcag ttgctggcgt
      121 tcaccgactg ctcgggctac cagacccgtt atgaatacga ccgctttggt cagatgacgg
      181 cggtccaccg tgaggaaggt atcagccgtt accgccgcta tgacaaccgt ggccggttaa
      241 cctcggtgaa agacgcacag ggccatgaaa cgcggtatga gtacaacgcc gcaggcgacc
      301 tgactgccgt tatcactccg gacggcaacc ggagcgagac acagtacgat gcgtggggaa
      361 aagcggtcag caccacgcag ggcgggctga cgcgcagtat ggagtatgac ct
//
LOCUS       DAZFDC010000493          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFDC010000493 DAZFDC010000000
VERSION     DAZFDC010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.496"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>410)
                     /locus_tag="ACHJ48_005333"
     CDS             complement(<1..>410)
                     /locus_tag="ACHJ48_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO4710630.1"
                     /translation="LTSEEVILTYPAGKCLKLLLDNAYSVVSHNQFFEKVWSDSSADV
                     ATNTLYQNISMIRRSFREISSATIFNDVIRTVPRKGFKINEEISITVLDNNHNENESV
                     ESEENGTHTENINKNHIAKGIFTTKNTLLIVCFF"
BASE COUNT          117 a           74 c           71 g          148 t
ORIGIN      
        1 caaaaaaaca aactataagt aatgtattct ttgtggtaaa tatacctttt gcaatgtgat
       61 ttttatttat attttcagtg tgggtaccat tctcttcact ctcaacactt tcattctcat
      121 tgtgattatt gtcgagaaca gttatgctga tttcttcatt gattttgaag cctttacggg
      181 gcaccgttct gataacatca ttaaatatcg tcgctgagct gatttcacga aaagaacgtc
      241 ttatcatcga tatattttga tataaagtgt tggttgcgac atcggcactg ctatcgctcc
      301 agactttttc aaagaattga ttgtgtgaca cgacactata agcgttatct aaaagtaact
      361 ttaagcattt tcccgcagga taagtgagta tcacttcctc tgatgtaagg
//
LOCUS       DAZFDC010000494          405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFDC010000494 DAZFDC010000000
VERSION     DAZFDC010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.497"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..297
                     /locus_tag="ACHJ48_005334"
     CDS             <1..297
                     /locus_tag="ACHJ48_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710631.1"
                     /translation="MLWVNKINILCVLFGINILYLGLKNQMVSIKQPQNIPSKQVKNK
                     TAPEQLITTENAFSEPPKLLRILFIAHVNALYNSSSPRYLTSLLFNYNSNKSRH"
BASE COUNT          148 a           84 c           51 g          122 t
ORIGIN      
        1 atgctatggg ttaataaaat caatatatta tgtgttttat ttggaataaa tattctatat
       61 ttaggattga aaaatcagat ggttagcatc aaacaacctc agaatattcc aagcaaacag
      121 gttaaaaata aaactgcacc cgaacaattg ataacgacag aaaacgcctt ttccgagcca
      181 ccaaaattac ttcgcatcct atttattgct cacgttaacg ccttgtataa ctcgagctct
      241 ccacggtatt taacctctct tctgtttaac tataattcca ataaatctcg tcactgagct
      301 caatcacttc gaaagtcaag cacaatatga atttcacata agctatcaat gaaaatcgga
      361 ggtcatacca ttaatcaaca ataaaccaag tctttctcat ataat
//
LOCUS       DAZFDC010000495          396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFDC010000495 DAZFDC010000000
VERSION     DAZFDC010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.498"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>396
                     /locus_tag="ACHJ48_005335"
     CDS             <1..>396
                     /locus_tag="ACHJ48_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4710632.1"
                     /translation="KGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLAL
                     EAELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETL
                     EYKRQLAELGDKVEYQKRLNELAQQAVRFE"
BASE COUNT          117 a           90 c          132 g           57 t
ORIGIN      
        1 aaaggtccgg ggcagcagga agccgtcagc cgtctggctg aagagctgac aggtaaaaag
       61 catacatcac cgaaaacgcg ctctgccggg gagagggaag aggagcaggc aagagaggct
      121 ctgcttgccc ttgaagctga gctcaggacg ctggaaaaac acagcggtgc gaatgagaaa
      181 atcagccggc agcgccgtga tttatggaag gcggaaagtc agtatgcggt cctgaaagag
      241 gctgccacga aacgacagtt atctgagcag gaaaaatccc tgctggcgca taaagacgag
      301 acgctggagt acaaacgcca gctggctgag ctgggcgaca aggttgaata ccagaaacgc
      361 ctgaatgagc tggcacagca ggcggtgcgg tttgaa
//
LOCUS       DAZFDC010000496          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFDC010000496 DAZFDC010000000
VERSION     DAZFDC010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.499"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>395)
                     /locus_tag="ACHJ48_005336"
     CDS             complement(<1..>395)
                     /locus_tag="ACHJ48_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO4710633.1"
                     /translation="PEDRISHLELDGFNYFSQPELDTPISDSFSYLYDNFQIVDSDVH
                     IKLLHLNRETKQITPHRIILKRYFIDSFAKTSITDREKNIYPVICDSPDHFTSDIYRH
                     PFRIVLGNKTLYPSEELVKFISTSKEYLS"
BASE COUNT          126 a           57 c           70 g          142 t
ORIGIN      
        1 gcttaaatat tcttttgagg tgcttatgaa ttttacaagt tcttctgatg gatacaatgt
       61 cttattcccg agaactattc taaatggatg cctatatatg tcgcttgtga agtgatcagg
      121 tgaatcacaa atcacaggat atatattctt ttctctgtca gtgatacttg ttttagcaaa
      181 tgagtctata aagtatcttt taagaataat acgatgtggt gttatttgct ttgtttctct
      241 gttgagatga agaagcttaa tatgcacatc actatcaacg atctgaaaat tatcatataa
      301 atatgaaaat gagtcagata ttggagtatc taactcgggt tgtgaaaaat aattaaaccc
      361 atctaactcc agatgagaaa ttctatcttc aggtg
//
LOCUS       DAZFDC010000497          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFDC010000497 DAZFDC010000000
VERSION     DAZFDC010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.500"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>390)
                     /locus_tag="ACHJ48_005337"
     CDS             complement(<1..>390)
                     /locus_tag="ACHJ48_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO4710634.1"
                     /translation="GDAYANPLSDDDWQRLAKVKDLTPGEMTAEPYDDNYLDDEDADW
                     TATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVS
                     SIGKAVTAKEVITRTVKVTNVGKPSVA"
BASE COUNT           71 a          135 c           85 g           99 t
ORIGIN      
        1 tccgccacgg aaggtttgcc cacgttagtg actttcaccg tgcgggtgat cacttctttc
       61 gccgtcacgg ccttaccgat actgctgacc cagccacgga acacatccac cgtgccattt
      121 gggaaacgga ttttataggc ccgcacatcc ccgctttcaa accagcctat aagccctttc
      181 tgaccttctt ctcccggttt ccaggccagc gtaaaactgg tatctcctgc agacttctgc
      241 ccctgcccgg tcgcggtcca gtccgcgtct tcatcatcca ggtagttatc atcgtagggt
      301 tctgccgtca tctcgcccgg cgtcagatcc ttcaccttag ccagtcgctg ccagtcatcg
      361 tctgacaacg ggtttgcata agcatcaccc
//
LOCUS       DAZFDC010000498          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFDC010000498 DAZFDC010000000
VERSION     DAZFDC010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.501"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>389
                     /locus_tag="ACHJ48_005338"
     CDS             <1..>389
                     /locus_tag="ACHJ48_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710635.1"
                     /translation="LSDFSSQLPREDGRKSAERIILIEYKENEQEVVPKQITDQQLQI
                     TYTSVKTDNYKQIYDEISAVDEGLSPYDVLRYQRAIKTLIINEGEKGHLDTLLVSPSD
                     LDRLEESVKQGKNLVVALGDKKYVFTQV"
BASE COUNT          149 a           60 c           70 g          110 t
ORIGIN      
        1 ctttctgatt tttcctcaca actacctaga gaagatggtc gaaaatcagc tgaaagaata
       61 attttgattg aatataaaga gaatgaacaa gaagtagtac caaaacagat taccgatcaa
      121 cagctacaaa ttacatatac atctgttaaa acagataact ataaacagat ctatgatgag
      181 atcagtgctg ttgatgaagg gctctcacct tatgatgttt taaggtatca aagagccatt
      241 aaaacactta tcattaatga gggtgaaaaa gggcacttag atactctatt ggtttcacct
      301 tctgacttag acagacttga agaaagtgtt aaacaaggaa aaaatttagt tgttgctcta
      361 ggcgataaaa agtatgtatt cacacaagt
//
LOCUS       DAZFDC010000499          387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFDC010000499 DAZFDC010000000
VERSION     DAZFDC010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.502"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..313
                     /locus_tag="ACHJ48_005339"
                     /pseudo
     CDS             1..313
                     /locus_tag="ACHJ48_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
     gene            310..>387
                     /locus_tag="ACHJ48_005340"
     CDS             310..>387
                     /locus_tag="ACHJ48_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO4710636.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLM"
BASE COUNT           88 a           83 c          136 g           80 t
ORIGIN      
        1 acgggatgcg gggatgggaa tgcctggttg acggggtggc ggatattgac gtcagcatga
       61 cgggtgaacg gtcgttctct gtggtggtcc ggcagagcag tggccagcgt acggaaaaaa
      121 cattttccct gccggtgatg ctctaccgtg gtgtgttcag aatcggcgaa acttaccacc
      181 ccggcgatac ggtgacgtgg ggggctcgtt gtggcactgc aacagtatga ccggtgataa
      241 gcccggagaa gctcattcat caggctggac tctggctgca aaacgtgggc gggatgcagg
      301 aggtggaaag tgacagcatt actgacactg gaagaaatca aggcacatct gcgtgttgac
      361 catgacgcgg atgatgagat gctgatg
//
LOCUS       DAZFDC010000500          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFDC010000500 DAZFDC010000000
VERSION     DAZFDC010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.503"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>378
                     /locus_tag="ACHJ48_005341"
     CDS             <1..>378
                     /locus_tag="ACHJ48_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710637.1"
                     /translation="KLRHTVTEFDESGKSEPTGLFTWYGKDKKGDSLAIVIKNKNGND
                     YLSLGYYDQDGYHIQKGFRIDGDNLTQYCSKKARSASAWFEHSKAIIAESFATGSDHQ
                     VVNELKGERLRELNEAFNPSNLA"
BASE COUNT          137 a           69 c           78 g           94 t
ORIGIN      
        1 acaaactcag acatacggta acggaattcg atgagagcgg gaaaagcgaa ccaacgggct
       61 tatttacctg gtacggtaaa gataaaaaag gcgactctct cgctattgta attaaaaata
      121 aaaatggaaa tgattactta tctctcggtt actacgatca ggatggctac cacattcaaa
      181 aaggatttcg tattgatggt gataatctca cccaatattg tagtaaaaaa gccaggagtg
      241 cttctgcgtg gtttgaacac agtaaagcaa tcattgcaga atcatttgca acaggttccg
      301 atcatcaagt tgtaaacgaa ctcaaagggg aaagactgag agaacttaac gaagctttta
      361 atccttcaaa tctagcaa
//
LOCUS       DAZFDC010000501          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFDC010000501 DAZFDC010000000
VERSION     DAZFDC010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.504"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>378
                     /gene="tccP2"
                     /locus_tag="ACHJ48_005342"
     CDS             <1..>378
                     /gene="tccP2"
                     /locus_tag="ACHJ48_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO4710638.1"
                     /translation="FFSGLPRSISSAIRSSTFTVSQHKSTPNTVKTSSPFSPSNSPAS
                     ATTIFKVKNSYTESGLQRPTSYTQSSIEKNALHRPLPDVAQRLVQHLAEHGIQPARSM
                     AEHIPPAPNWPAPPPPVQNEQSRP"
BASE COUNT          111 a          112 c           64 g           91 t
ORIGIN      
        1 tttttttccg gccttcctcg cagtatatca agtgctatac gcagcagtac cttcactgtc
       61 tcgcagcaca aaagcacacc taacacggta aaaaccagct cacctttttc tcccagcaac
      121 agtcctgcca gtgcaacaac tatttttaag gtaaaaaatt cttatacaga atcaggactt
      181 caacgtccca catcttatac tcagtcaagt atagaaaaaa acgctttaca tcggcctcta
      241 cctgatgtag ctcagcgtct ggtgcagcat cttgcagagc atggcattca accagcccgg
      301 agtatggctg aacatattcc tccggcacct aactggcctg cgccaccacc gccagtacaa
      361 aatgaacaat caagacct
//
LOCUS       DAZFDC010000502          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFDC010000502 DAZFDC010000000
VERSION     DAZFDC010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.505"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..144
                     /locus_tag="ACHJ48_005343"
     CDS             <1..144
                     /locus_tag="ACHJ48_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710639.1"
                     /translation="IQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQR
                     IWR"
     gene            146..>375
                     /locus_tag="ACHJ48_005344"
     CDS             146..>375
                     /locus_tag="ACHJ48_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710640.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAG"
BASE COUNT           82 a          106 c          103 g           84 t
ORIGIN      
        1 attcagtgtt accgcaggcg cggatgcgag gttaaaaaag cccttagcct cgactaccgt
       61 cactggataa tctccgtcag gctcccttac tccgaacggc caccgcgtcc gtcccgcaca
      121 ttccagcaac ggatctggag gtaatgtgcg ggtattactt cgacctgttc tggtaccgga
      181 actcggtctg gttatcgtta agccaggccg tgaatcaatg tcagcattcc ataacggcag
      241 aatactggtg gagccggaac caaaaagcat gcgagctctg ccgtccgggg ttgtacctgc
      301 cgttcaccag ccgctggcgg aagataaatc actactgcca tttttcagcg atgagcgggt
      361 gatccgtgct gcggg
//
LOCUS       DAZFDC010000503          375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFDC010000503 DAZFDC010000000
VERSION     DAZFDC010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.506"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>375
                     /locus_tag="ACHJ48_005345"
     CDS             <1..>375
                     /locus_tag="ACHJ48_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO4710641.1"
                     /translation="TNINTACVKNNASYQFNNALPNKETISSNFCERLEQWGNKSLNN
                     GEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFTDNA
                     SLVNINLSFNKINTITFPNESN"
BASE COUNT          133 a           72 c           59 g          111 t
ORIGIN      
        1 tgaccaatat taatacagct tgtgtaaaaa ataatgccag ttatcaattt aataacgcat
       61 tacctaacaa agaaaccatc tccagcaatt tttgtgaacg actagaacaa tggggtaata
      121 agtcgcttaa taatggtgaa gaaagagcaa ttgccgtgga gcgaattaaa gaagcttaca
      181 attcgaacat ggcatctctt gatttgtcat atcttgattt gagtgagtta ccgcctatcc
      241 cctcgacagt taatacgtta aatctggaaa acaattgtct aacttgtctt gactttactg
      301 acaatgccag tctcgttaat atcaacctca gctttaataa aattaacacg ataacctttc
      361 caaatgaatc aaatc
//
LOCUS       DAZFDC010000504          373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFDC010000504 DAZFDC010000000
VERSION     DAZFDC010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.507"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>373)
                     /locus_tag="ACHJ48_005346"
     CDS             complement(<1..>373)
                     /locus_tag="ACHJ48_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO4710642.1"
                     /translation="FDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVG
                     RIGSAIGGAFGGGASASSGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTK
                     EATSRIGVGNLYRLMRGYAEGG"
BASE COUNT           84 a          133 c           87 g           69 t
ORIGIN      
        1 cccccttccg catagccacg catcagacgg taaagattcc ccacacctat ccggctggtt
       61 gcctctttcg tgaaaacaaa ctccccgcgg tgaactatcc ccgcaggctc atatttgccg
      121 ccggttcccg taaatccgcc ggtcgcaaaa tggaagttcg ccgccgcagc ctgaatggcc
      181 gtccccgagg aagcagatgc accaccaccg aaagcaccgc caatggcgct gccgatacgc
      241 ccgacaatgc ccaccatggc ctgtttaagc aggatttctg tcatcatgga cagcaccgaa
      301 cgggtgaatc cccgccagtc tgcctctgca ccggtcagca tcgccgccat attctgtgca
      361 ataccgtcaa agg
//
LOCUS       DAZFDC010000505          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFDC010000505 DAZFDC010000000
VERSION     DAZFDC010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.508"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>368
                     /locus_tag="ACHJ48_005347"
     CDS             <1..>368
                     /locus_tag="ACHJ48_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO4710643.1"
                     /translation="DWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
                     VMGLAGVLRGKKVRTTVSRKAVAACDRVNRQFVAERPDQLWVADFTWVSTWQGFVYVA
                     FIIDVFAGYIVGWQVSSSMET"
BASE COUNT           84 a           82 c          115 g           87 t
ORIGIN      
        1 gactggctga agaaagagat acagcgcgta tacgatgaaa atcaccaggt atacggtgtg
       61 cgtaaagtct ggcgtcagtt gttacgggaa ggtatcagag tggccagatg cactgtggca
      121 cgtctcatgg cagttatggg acttgccggt gttctccggg gtaaaaaggt ccgcactacc
      181 gtcagccgga aagccgttgc cgcatgcgac cgcgtaaacc gtcagttcgt ggcagaacgt
      241 ccagaccagt tgtgggtggc tgattttacc tgggtaagca catggcaggg cttcgtttat
      301 gtggcgttca tcattgatgt gttcgccgga tacatcgtgg gatggcaggt ctcatcatcc
      361 atggaaac
//
LOCUS       DAZFDC010000506          354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFDC010000506 DAZFDC010000000
VERSION     DAZFDC010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.509"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..203)
                     /locus_tag="ACHJ48_005348"
     CDS             complement(<1..203)
                     /locus_tag="ACHJ48_005348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710644.1"
                     /translation="MCRKYAHVVDALQGNFPYATDPAGNRLPDPELHPDSTLTAWPDN
                     RIAEDAHYVYRYDEYGRLAEKTDR"
BASE COUNT           74 a           90 c           83 g          107 t
ORIGIN      
        1 cggtccgtct tctccgccag cctgccgtat tcatcgtagc ggtagacata gtgcgcatct
       61 tccgcgatgc ggttatccgg ccatgccgtg agggtgctgt ccgggtgcag ttccgggtcc
      121 ggcagccggt ttcctgccgg gtctgttgca tacgggaaat tcccttgcag ggcgtccaca
      181 acatgtgcat atttcctgca catgctgtgg ttttgtcata caaggagcaa caatactagt
      241 taatgattca taaaaaattt taacggggtg atttttcatg cgattatcgt cctaattaac
      301 gccacctcac ttacagatta tttcattcgt tacaatcttt tgattgcatt acac
//
LOCUS       DAZFDC010000507          351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFDC010000507 DAZFDC010000000
VERSION     DAZFDC010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.510"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>351
                     /locus_tag="ACHJ48_005349"
     CDS             <1..>351
                     /locus_tag="ACHJ48_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO4710645.1"
                     /translation="KAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISDSFGGPLS
                     LLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAAGGGVAVL
                     AALAAAAWQAEQAD"
BASE COUNT           66 a           97 c          115 g           73 t
ORIGIN      
        1 ataaggctgc catgcgcaca ctgcccgcac agttcacgga tattgtcact cagcttgccg
       61 gtggtcagaa tcccttcctt atcatgctgc agcagggggg gcagatcagc gattcattcg
      121 gtggaccgct cagcctgctt accctgctga aggaggaact tctcgggatc agggatgcct
      181 ctgaatcatc agaggagtcg ctgtcagata cggcaaatgc actggctgaa aatgcccgga
      241 atgccggtga gctgggacga tttatgtcgg tggcccgtgt ggcggcaggt ggcggggttg
      301 ccgtactggc cgcgcttgct gccgccgcct ggcaggcaga gcaggctgac c
//
LOCUS       DAZFDC010000508          351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFDC010000508 DAZFDC010000000
VERSION     DAZFDC010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.511"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..96
                     /locus_tag="ACHJ48_005350"
     CDS             <1..96
                     /locus_tag="ACHJ48_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO4710646.1"
                     /translation="GEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          102 a           61 c          112 g           76 t
ORIGIN      
        1 ggtgaaatgc tggttggttc acgacggata tcccaggaca tcagcacccg tgatgagggc
       61 ggagacggga aagtggtggt tatcgggcgg ggatgaaaat aaaaaaatcc cgcagagtta
      121 gcggagctgc gggagagaac gatgaagatt aacgttatgg agttattttt caggcatcaa
      181 aaaagtaatg cagcgtcatt attgcggcta caggcaattg ccggaaatgt gaagagtttc
      241 agaaatttta ttccgtcatg acacaggcac cctccggggt gcctgttgtt ttctggcata
      301 aacagattca gacatcagac aggagagggg gacagagtgg gtaaaggggg c
//
LOCUS       DAZFDC010000509          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFDC010000509 DAZFDC010000000
VERSION     DAZFDC010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.512"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          109 a           73 c           64 g          102 t
ORIGIN      
        1 aaaaatcccc gttcccggag aaataatatt tattaaaatt ccaggtcttc tttttctgat
       61 taccgaagac aaagtggcaa ttacgtatag tttcccgata aagacgcgac agcgacatcc
      121 cgcataaggc attttttctt tatctttgta cggtacttca tggaacagag tttttgacct
      181 tgcgaatcgt gatgtctgtt gggggcaatt tactcactga agcgtgagac tcgattaagc
      241 gcacgaaaca cagaaatcaa aaaacccggt cactttttac aaggcaaccg ggtaaaaata
      301 atttttattt tttaactgcc ctgagactca taaagatgcc tcaaaact
//
LOCUS       DAZFDC010000510          346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFDC010000510 DAZFDC010000000
VERSION     DAZFDC010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.513"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>346
                     /locus_tag="ACHJ48_005351"
     CDS             <1..>346
                     /locus_tag="ACHJ48_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HFO4710647.1"
                     /translation="GFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLAL
                     THSTSDFGNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQ
                     VREGAEYKYITTS"
BASE COUNT           81 a           79 c          112 g           74 t
ORIGIN      
        1 ccggatttga aaatgtcgag aaggataacg cctataacgg gatgaccctg cgtgaatggg
       61 ctcgcatgtc actgacggag cgcggtattg gggtggccag ttataacccc atgcagatgg
      121 tcgggctggc gctgacgcac agcacctctg attttggcaa tattctgctg gatgtgtcga
      181 acaaggggct gatccagggc tgggaggaat cagaagaaac cttccagaag tggacccgta
      241 agggacgcct gtcagacttc aaaacagcgt atcgcgtggg gatgggcggt tttggttctc
      301 tgcgccaggt tcgtgagggg gcggagtata aatacatcac cacctc
//
LOCUS       DAZFDC010000511          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFDC010000511 DAZFDC010000000
VERSION     DAZFDC010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.514"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>341
                     /locus_tag="ACHJ48_005352"
     CDS             <1..>341
                     /locus_tag="ACHJ48_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO4710648.1"
                     /translation="LVFHTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSL
                     SRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAE
                     VLQEDTGVTLPA"
BASE COUNT           79 a           90 c          126 g           46 t
ORIGIN      
        1 ctggtgttcc acacgcggat acagcacggc gaaccacagg tggagagccg gtacctctat
       61 gacccgctgg gccgccggac gggaaaacgg gtgtggcggc gggagcgtga cctgacgggg
      121 tggatgtcgc tgtcgcgtaa accggaggag acctggtacg ggtgggacgg tgacaggctg
      181 accactgtac agacccaaca gacaagaatc cagacggtat accagccggg aagcttcacg
      241 ccgctcctga gaatcgaaac agagaatggt gaacaggcga aggcgcggca ccgtagcctg
      301 gcggaggtgt tgcaggagga cacgggtgtg acgctaccgg c
//
LOCUS       DAZFDC010000512          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFDC010000512 DAZFDC010000000
VERSION     DAZFDC010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.515"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>333
                     /locus_tag="ACHJ48_005353"
     CDS             <1..>333
                     /locus_tag="ACHJ48_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO4710649.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAK"
BASE COUNT           74 a           85 c          103 g           71 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaa
//
LOCUS       DAZFDC010000513          325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFDC010000513 DAZFDC010000000
VERSION     DAZFDC010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.516"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>325)
                     /gene="nleC"
                     /locus_tag="ACHJ48_005354"
     CDS             complement(<1..>325)
                     /gene="nleC"
                     /locus_tag="ACHJ48_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO4710650.1"
                     /translation="NTVLDALSRSQTFRDAVSYGIHNEEVHIGCIKYRNEYELNGESA
                     IKIDDIQSLTCNELYGYDVGQEPILPICEAGENENEEPYVSFSVAPDTDSYEMPSWQE
                     GLIHEI"
BASE COUNT           84 a           75 c           61 g          105 t
ORIGIN      
        1 taatctcgtg aatcagtcct tcctgccacg atggcatctc ataagaatca gtatctggcg
       61 caacactaaa actgacataa ggctcttcgt tctcgttttc tcctgcctcg caaatgggga
      121 gaattggttc ttgcccgaca tcgtatccat ataattcgtt acaggttagt gattgaatat
      181 catcaatttt gatagcagat tctccgttaa gctcgtattc gtttctgtat ttaatgcaac
      241 caatgtgtac ctcctcatta tgaatgccat agcttacggc atcacgaaat gtttgtgatc
      301 ggctcaacgc atcaagtaca gtgtt
//
LOCUS       DAZFDC010000514          316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFDC010000514 DAZFDC010000000
VERSION     DAZFDC010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.517"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            8..>316
                     /locus_tag="ACHJ48_005355"
     CDS             8..>316
                     /locus_tag="ACHJ48_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO4710651.1"
                     /translation="MNNTLLPSINIPCTLFETISLFDNYSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     F"
BASE COUNT          103 a           58 c           46 g          109 t
ORIGIN      
        1 aatgtaaatg aataacacat tacttccatc aattaatatt ccctgtacac ttttcgaaac
       61 tattagttta tttgataatt attctgctga tgatatgcaa tatggtgata tggtagaaca
      121 agattttctc tcactcggcc taagcgatat ctcagcaaaa gttgatcctt acagacttat
      181 taaataccat ttccctggcc caggttctat taatgttgct tttagtgcct caagttccgg
      241 aacaaaaata tcacaacgtg aatgcactga tattttattt gcagaaatga aagaactagc
      301 aaaaatgttt tccttt
//
LOCUS       DAZFDC010000515          315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFDC010000515 DAZFDC010000000
VERSION     DAZFDC010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.518"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(145..>315)
                     /locus_tag="ACHJ48_005356"
     CDS             complement(145..>315)
                     /locus_tag="ACHJ48_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO4710652.1"
                     /translation="PADILESDENGIIPEQDGVITQVVILDADKKQIQCVVRPLQILR
                     ADGRWENIGGMK"
BASE COUNT           77 a           89 c           55 g           94 t
ORIGIN      
        1 atacatctaa gcgctcgtga ttataatata attacctata ttagctctga cctgacagtt
       61 accggttatt tatacaggta tctgtcagat tacatctaac caacaaaaaa ccggagccgg
      121 actccggttt tgtgaagctg tcggctattt cattccgcca atattttccc acctcccgtc
      181 agcacgcaga atttgcagcg gtcttaccac acactgtatc tgctttttat ctgcatccag
      241 tatcaccacc tgcgtgatta ccccgtcctg ctccggaata atgccattct catctgactc
      301 caggatgtct gccgg
//
LOCUS       DAZFDC010000516          312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFDC010000516 DAZFDC010000000
VERSION     DAZFDC010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.519"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..75
                     /locus_tag="ACHJ48_005357"
     CDS             <1..75
                     /locus_tag="ACHJ48_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO4710653.1"
                     /translation="NDVLMQKAAGLAGGVRFGEEGRRL"
     gene            119..>312
                     /locus_tag="ACHJ48_005358"
     CDS             119..>312
                     /locus_tag="ACHJ48_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710654.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALA"
BASE COUNT           73 a           61 c          108 g           70 t
ORIGIN      
        1 aatgatgtac tgatgcagaa ggcggcgggc ctcgccggtg gagtccgttt cggggaggag
       61 ggaaggaggt tgtgacagtt attgatggta tcagaggaca tttcaggagg tgaccacgat
      121 ggcaggtaat tttgccgatc tgacagccgt gctgacactg gattctgccc gtttttctga
      181 agaggcagcg cgggtaaaaa aagagctggg tgaaaccagt gcgcttgctg atttgatgtc
      241 cgggaaagtc agtcagtctt tcagaaaaca ggctgatgct gctgagcaga gtctgagccg
      301 acaggcgctg gc
//
LOCUS       DAZFDC010000517          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFDC010000517 DAZFDC010000000
VERSION     DAZFDC010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.520"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..286
                     /locus_tag="ACHJ48_005359"
     CDS             <1..286
                     /locus_tag="ACHJ48_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710655.1"
                     /translation="QWEQKFARHVQHVRAQVKPVSKGVSHAAPGGTASRAVQEIRAAR
                     EQWERENGFISNGNGLEAVGTYGGGVFEPLDPEERGCAVEALDCSDWRDD"
BASE COUNT           82 a           72 c          102 g           50 t
ORIGIN      
        1 tcagtgggag cagaaatttg cccgtcacgt gcagcacgtc agggcacagg taaaaccagt
       61 cagcaagggg gtaagccatg cagcaccagg tggcaccgca tcacgggcag ttcaggaaat
      121 tcgggcagca cgtgaacagt gggaacgtga aaacggattt atcagcaacg gaaacggcct
      181 ggaagctgtg ggaacttatg ggggaggtgt attcgaaccg ctggacccag aagaacgggg
      241 ctgcgccgtc gaagctctgg attgctcaga ttggcgcgat gactgaacag caaatccggc
      301 aggtct
//
LOCUS       DAZFDC010000518          304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFDC010000518 DAZFDC010000000
VERSION     DAZFDC010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.521"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..268
                     /locus_tag="ACHJ48_005360"
     CDS             <1..268
                     /locus_tag="ACHJ48_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO4710656.1"
                     /translation="LETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADA
                     LNQATNRVLYLSGMLDNRHDADPEPTGKTEATEPVTSKKHSKAS"
BASE COUNT           77 a           77 c           89 g           61 t
ORIGIN      
        1 tctggaaacg gcatccctgc ttgtggcgat gtcgctatgg cattcccatt ccctcaaagg
       61 cactctggcc tctccggaga cagagatgca gcagatccgc cgtgaagtga tgctgggatg
      121 gcctgctgat gcactgaatc aggcaacgaa ccgggtgctt tatctttcag gtatgctgga
      181 taaccggcac gatgccgatc ctgaaccaac cgggaaaaca gaagcgactg agccggtaac
      241 atcaaaaaag cattcgaagg cgagctgaac tttgtcctga aactggcgcg tgagatgggg
      301 agag
//
LOCUS       DAZFDC010000519          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFDC010000519 DAZFDC010000000
VERSION     DAZFDC010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.522"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>303
                     /locus_tag="ACHJ48_005361"
     CDS             <1..>303
                     /locus_tag="ACHJ48_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO4710657.1"
                     /translation="DKGSKVQLEVYNLLSEEKIFEHKIMNNWTRSIKNILTTYLFMSS
                     GAVTARNVQTFSPTINNESRIRLPRALPVGHPYPEEHKASGFSPFMMGGLSGDILP"
BASE COUNT          102 a           48 c           67 g           86 t
ORIGIN      
        1 cgataaagga agcaaagttc aattggaggt atataattta ttatccgaag aaaagatatt
       61 tgaacataaa attatgaata attggacaag gagcataaaa aatatattga cgacatattt
      121 gtttatgtca tcaggagcgg tgacagccag aaatgttcaa accttttctc caacaataaa
      181 taatgagtca aggattcgat tgccgagagc attgccagta ggccatccat atcctgagga
      241 acataaggct tctggtttct ccccttttat gatggggggg ctgagtggtg atattcttcc
      301 gga
//
LOCUS       DAZFDC010000520          302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFDC010000520 DAZFDC010000000
VERSION     DAZFDC010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.523"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(135..>302)
                     /locus_tag="ACHJ48_005362"
     CDS             complement(135..>302)
                     /locus_tag="ACHJ48_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M85"
                     /protein_id="HFO4710658.1"
                     /translation="DFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPY
                     IFTFGDVDRHN"
BASE COUNT           93 a           73 c           54 g           82 t
ORIGIN      
        1 cgccaggccc acttacaaca gtaaaattcg gggctctgcc tttacataca atacaatgtt
       61 gcacgattgc ccatcatcaa ctccgttaat atctatgagt agtaacccta tgtaactgta
      121 aagacgaatc agcgctaatt gtgtctgtcc acatccccaa atgtaaagat ataaggagca
      181 ccatgataca gttggatctg atttacatcc tgtaaattct cgtcgatagc cacccctgga
      241 aaatcatgtt gctggataaa tccgtatcct atgttagaca cagcggaata ctctgtgaaa
      301 tc
//
LOCUS       DAZFDC010000521          292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFDC010000521 DAZFDC010000000
VERSION     DAZFDC010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.524"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>292
                     /locus_tag="ACHJ48_005363"
     CDS             <1..>292
                     /locus_tag="ACHJ48_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFO4710659.1"
                     /translation="LNLNHCCLYAACLGELTGGNPLYDCAIDTHTTILSGNLAANTQV
                     KIPVYMYHRGAIAEKDILPFMEKVTNIYSRIEDIYKYSNNMKNNYKKALADLQ"
BASE COUNT           99 a           44 c           52 g           97 t
ORIGIN      
        1 ttaaatctta atcattgctg tttatatgct gcttgtttgg gcgagttaac cggtgggaat
       61 cccttgtatg attgtgctat tgatactcat accactatct taagtggtaa tcttgctgca
      121 aacactcagg ttaaaattcc tgtttatatg taccatagag gagctatagc tgaaaaagac
      181 attttacctt tcatggaaaa ggtaacgaat atttattcac ggatagaaga tatttataaa
      241 tatagcaata atatgaagaa taattacaaa aaggcactag ccgatttaca at
//
LOCUS       DAZFDC010000522          290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFDC010000522 DAZFDC010000000
VERSION     DAZFDC010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.525"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>290
                     /locus_tag="ACHJ48_005364"
     CDS             <1..>290
                     /locus_tag="ACHJ48_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710660.1"
                     /translation="CSFENSGSYMVDVYRTVKRVNETIQTPNINCYTTCILTRPANGH
                     VTSTGFSRNLMDAINISLAEHKIASLHNEVMSIEEYDRDSLQEKGNTARRYL"
BASE COUNT          105 a           48 c           54 g           83 t
ORIGIN      
        1 atgttcattt gaaaactcag gttcttatat ggtagacgtt tatagaacgg ttaaaagagt
       61 aaatgagaca atccagaccc caaatataaa ttgctatact acatgtattt taacaaggcc
      121 cgcaaatggt catgttacat ctacaggctt ctcacgaaac ctcatggatg cgataaatat
      181 ttcattagca gagcacaaaa tagcctcttt acataatgaa gtcatgagta ttgaggaata
      241 tgatcgtgat tctttacaag agaaaggaaa tactgccagg agatatttag
//
LOCUS       DAZFDC010000523          289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFDC010000523 DAZFDC010000000
VERSION     DAZFDC010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.526"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>289)
                     /locus_tag="ACHJ48_005365"
     CDS             complement(<1..>289)
                     /locus_tag="ACHJ48_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO4710661.1"
                     /translation="GELLSSLINDNKGNKQLSDNASKIINLLGIEYQSHKVDIEPFIH
                     AVWVAGAPPDNTFSYITAFLNTYKDYTYLLWIDPNAFGAAKFSGILKNIAMN"
BASE COUNT           89 a           52 c           43 g          105 t
ORIGIN      
        1 attcatcgct atatttttta aaatcccact aaacttggcc gcaccaaagg cattaggatc
       61 aatccagagc aggtaggtat aatctttata tgtatttaaa aatgccgtta tatatgaaaa
      121 tgtattatca ggaggtgcgc cagcaaccca tactgcatgt ataaatggct ctatgtctac
      181 tttatgtgac tgatactcta tacccaataa gtttattatt ttagatgcat tgtctgataa
      241 ttgtttattt cctttattat catttattaa tgatgataac agctctccc
//
LOCUS       DAZFDC010000524          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFDC010000524 DAZFDC010000000
VERSION     DAZFDC010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.527"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>281
                     /locus_tag="ACHJ48_005366"
     CDS             <1..>281
                     /locus_tag="ACHJ48_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HFO4710662.1"
                     /translation="RNMITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVM
                     AEQKARMSGINDLFAMFGGRYQTLQAQCVADPDCSLEMARERLLNEMGK"
BASE COUNT           65 a           81 c           79 g           56 t
ORIGIN      
        1 cgaaacatga ttactccgcc acgcaacagt gccccgcgtg ataccacagt gacaatccct
       61 gcaccggcgg taacagaacc atcaccggta ccggcagtgt ctgatgaggc gaccattcgc
      121 gcccgcgtta tggctgagca gaaagcccgc atgtcaggca ttaacgatct gtttgccatg
      181 ttcggcggtc gctatcagac gcttcaggca cagtgcgtgg ctgatcctga ctgttcgctg
      241 gaaatggccc gtgaacgtct gctgaatgaa atgggcaagg a
//
LOCUS       DAZFDC010000525          279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFDC010000525 DAZFDC010000000
VERSION     DAZFDC010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.528"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>279
                     /locus_tag="ACHJ48_005367"
     CDS             <1..>279
                     /locus_tag="ACHJ48_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710663.1"
                     /translation="QGSFLIKCINSLTGLVGIPGLPDKEFALSTPEFMKSKQHKAFST
                     VFKDVTNELTSIERLGGLRDKLASNFTIDDSYYTEPKTEDPRFRYVSSN"
BASE COUNT           89 a           47 c           57 g           86 t
ORIGIN      
        1 caaggcagtt ttttgataaa atgtataaat tcattgacag gactcgtggg tattccaggt
       61 ttgcctgata aagagtttgc actgagcact cctgaattta tgaagtctaa acaacataaa
      121 gcattttcta ccgttttcaa ggatgttact aacgaactaa caagcattga acgtttaggt
      181 gggcttcggg ataaattagc gtcgaacttt actattgatg attcttatta taccgagcca
      241 aaaacagagg atccgagatt ccgttacgta agtagtaat
//
LOCUS       DAZFDC010000526          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFDC010000526 DAZFDC010000000
VERSION     DAZFDC010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.529"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            24..100
                     /locus_tag="ACHJ48_005368"
     tRNA            24..100
                     /locus_tag="ACHJ48_005368"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHJ48_005369"
     tRNA            143..218
                     /locus_tag="ACHJ48_005369"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZFDC010000527          277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFDC010000527 DAZFDC010000000
VERSION     DAZFDC010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.530"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>277
                     /locus_tag="ACHJ48_005370"
     CDS             <1..>277
                     /locus_tag="ACHJ48_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO4710664.1"
                     /translation="SKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAP
                     AEQTHVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGL"
BASE COUNT           87 a           45 c           52 g           93 t
ORIGIN      
        1 tcaaaattaa ttgacaagct tatcgaatat aaagatgagt atgatactgt tttattatat
       61 gtctctgacc atggagaatc tctgggagaa aatggattat acttacatgg aacaccatat
      121 aatgttgctc cagctgagca aacgcatgtg cctcttatta cctggatgtc tccggggttc
      181 gtttcctcta aaaaaattga tttgaattgt ttgtcagaac atgctctaaa cagaacagta
      241 agtcatgata atatattttc atcgttactg gggttat
//
LOCUS       DAZFDC010000528          275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFDC010000528 DAZFDC010000000
VERSION     DAZFDC010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.531"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            182..>275
                     /locus_tag="ACHJ48_005371"
     CDS             182..>275
                     /locus_tag="ACHJ48_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO4710665.1"
                     /translation="MAISSYSTVTDKFANLTKMPDENNGLQKNQK"
BASE COUNT          113 a           39 c           50 g           73 t
ORIGIN      
        1 aagaaaacaa aaaacagggt aaaagcccta tatcgagaca taaaaaatga gcgatttttt
       61 acagtattca taggtggttt agtcataatc gtagcaaacc aacacccagg tatgcttttt
      121 aagacaaatc ggcaatgtat tatgctcggg cgaataaata ttgtgttcag gagaaatgat
      181 tatggctata tcatcatata gtacagttac agataaattt gctaatctga ctaaaatgcc
      241 agatgaaaat aatggattgc aaaaaaatca gaaaa
//
LOCUS       DAZFDC010000529          270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFDC010000529 DAZFDC010000000
VERSION     DAZFDC010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.532"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>270
                     /locus_tag="ACHJ48_005372"
     CDS             <1..>270
                     /locus_tag="ACHJ48_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO4710666.1"
                     /translation="KDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSD
                     RGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE"
BASE COUNT           77 a           64 c           74 g           55 t
ORIGIN      
        1 caaagatgtt tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga
       61 gctgacaggt aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat
      121 ccaccactct gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca
      181 gtttggtctg aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga
      241 aagcttctgg ggaacgctga aaaatgagag
//
LOCUS       DAZFDC010000530          259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFDC010000530 DAZFDC010000000
VERSION     DAZFDC010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.533"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..226
                     /locus_tag="ACHJ48_005373"
     CDS             2..226
                     /locus_tag="ACHJ48_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFO4710667.1"
                     /translation="MYENIKQAISSAPRNSQTMEMHLQMIKYADHLKNVTAKEFCEGV
                     GLKASFATEFSKMRNLTERLKAAGLDTTKL"
BASE COUNT           94 a           59 c           51 g           55 t
ORIGIN      
        1 gatctacgag aacattaagc aagccattag cagcgcacca cgaaatagcc aaacgatgga
       61 aatgcacctc cagatgatca aatacgctga ccatctgaaa aacgtcacgg caaaagagtt
      121 ttgcgaaggt gtaggactta aagccagctt tgctacagag ttcagcaaaa tgagaaatct
      181 taccgagcga ctcaaagccg ccggacttga taccacgaag ttataacaaa cgaaatcatc
      241 aatatattac tttcgtttt
//
LOCUS       DAZFDC010000531          254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFDC010000531 DAZFDC010000000
VERSION     DAZFDC010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.denovo.534"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>254)
                     /locus_tag="ACHJ48_005374"
     CDS             complement(<1..>254)
                     /locus_tag="ACHJ48_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO4710668.1"
                     /translation="GTLNDFLGAMSEDDVRPEALRRFELMVEEVARHAEEAKKNAGEA
                     ETSARNAGISASQAEESAANADTSAGEASESARQAAESAAS"
BASE COUNT           39 a           96 c           59 g           60 t
ORIGIN      
        1 gaggctgcac tttctgccgc ctgccgggct gactccgatg cctcccctgc tgaagtgtca
       61 gcatttgcag cgctctcttc tgcctgactg gctgatatgc cggcattcct cgctgacgtc
      121 tccgcctctc cggcattctt cttcgcctcc tcagcgtgac gcgccacctc ttccaccatc
      181 agttcaaaac ggcgcagtgc ctccggccgg acgtcatcct ccgacatggc accgagaaaa
      241 tcattcagcg tacc
//
LOCUS       DAZFDC010000532         5506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.guided.208, whole genome shotgun sequence.
ACCESSION   DAZFDC010000532 DAZFDC010000000
VERSION     DAZFDC010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.guided.208"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..302
                     /locus_tag="ACHJ48_005375"
                     /pseudo
     CDS             99..302
                     /locus_tag="ACHJ48_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            301..822
                     /gene="prfH"
                     /locus_tag="ACHJ48_005376"
                     /pseudo
     CDS             301..822
                     /gene="prfH"
                     /locus_tag="ACHJ48_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHJ48_005377"
     CDS             complement(879..2336)
                     /gene="pepD"
                     /locus_tag="ACHJ48_005377"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO4710669.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2597..3055
                     /gene="gpt"
                     /locus_tag="ACHJ48_005378"
     CDS             2597..3055
                     /gene="gpt"
                     /locus_tag="ACHJ48_005378"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO4710670.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3147..4391
                     /gene="frsA"
                     /locus_tag="ACHJ48_005379"
     CDS             3147..4391
                     /gene="frsA"
                     /locus_tag="ACHJ48_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO4710671.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSCLLHQRVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4449..4850
                     /gene="crl"
                     /locus_tag="ACHJ48_005380"
     CDS             4449..4850
                     /gene="crl"
                     /locus_tag="ACHJ48_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO4710672.1"
                     /translation="MTLPSGHPKSRLIKKFTVLGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4889..>5506)
                     /locus_tag="ACHJ48_005381"
     CDS             complement(4889..>5506)
                     /locus_tag="ACHJ48_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO4710673.1"
                     /translation="RASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFG
                     TSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFY
                     SETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYI
                     DVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1332 a         1361 c         1432 g         1381 t
ORIGIN      
        1 tgaatcccca ggagcttaca taagtaagtg actggggtga acgaacgcag tcgcagtaca
       61 tgcaacttga agtatgacga gtataaatgg aatggtacat gggcaaatat attcgtccct
      121 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgatcc
      181 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtgggg atgccagatt
      241 tacaccccgg acgcggctac ccgattggcg cagcgttctt ctctgttggt cgtttttacc
      301 cgacaagacg tcgcggtaac ggtgctggaa acagaaacgg gccgctactc tgacacgctg
      361 cgttcggcgc tgatttctct ggatggcgac aacgcatggg cgttaagcga aagttggtgc
      421 ggcactattc agtggatttg tctgagtccg tatcggcctc atcatgggcg caaaaactgg
      481 tttctgggca ttgggcgttt taccgctgat gagcaggaac aatcggatgc aatccgttat
      541 gagacgctgc gtccgtcggg gccgggcggt caacatgtca ataaaaccga ctcggcggta
      601 cgcgccacgc atctggcaac cgggattagc gtgaaggttc agtcagaacg cagccagcat
      661 gctaacaaac ggctggcgcg attgctgatt gcctggaagc tggagcagca gcaacaggaa
      721 aatagcgcag tgctgaaatc gcagcggcga atgttccatc accagattga acgtggcaac
      781 ccgcgacgaa cgttcacagg gatggctttt atcgaaggat aatgaaggat gaaactgccg
      841 gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc
      901 agcagttcag tcagcagtgt ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc
      961 ggagagtgtg gaccggtgat tgttggcccg atagaaacca tgtccatttc cggatacggt
     1021 tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg cgtcttgttg
     1081 aacaggcgct gataggtttc acgtaccaga tgcatcaccg gagaattagc gtccggctgc
     1141 cagccaggat atgcgccttt cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc
     1201 agcatgctca ccacgtagtc tttaccgctg tcgatcagag aacggatcag acagtgaatt
     1261 tctacgttat tgtcagtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct
     1321 tttgccacgt cggagttgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag
     1381 gtatcgcgag atttcgcaat cagggcagct ttatcgttcg ctacagagtc cagcaacaag
     1441 gccagatttt tctctttctc tgccagctcg tttttcagga tctcctgata ggtattcacc
     1501 agagatttca gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc
     1561 gggatggcgt tacgcagtgt gccgccgttg aaatcaataa gacgcaggtc cagttcttct
     1621 gcatgacccg ccaggaagcg caccagcagt ttgttggcat tacccaggcc aacgtggatt
     1681 tccccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca
     1741 gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc ccccgcgcaa
     1801 cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac
     1861 cagttgctct gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc
     1921 acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc agaggccata
     1981 ccaatgccgt tatccgcacc cagcgtggtg ccgcgcgctt taacccattc gccatcaata
     2041 tatggctgga taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc
     2101 atatcgaggt gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt
     2161 ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc
     2221 caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag
     2281 attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta
     2341 agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt
     2401 tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     2461 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     2521 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     2581 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     2641 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     2701 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     2761 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     2821 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     2881 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tctttgcaaa
     2941 accggctggt cgtccgctgg ttgatgatta tgttgttgat atcccgcaag atacctggat
     3001 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     3061 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgagtta caatagtttc
     3121 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     3181 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     3241 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     3301 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     3361 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     3421 accgtggcgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
     3481 cctgtgcgga agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt
     3541 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     3601 tgtcaaaccg cgcctatgaa gaggccgctc agcgtctacc gggcacgatg cgacagatgg
     3661 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     3721 gcccgttccc gacggtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     3781 gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt
     3841 cggtgggctt ttcttcaaaa tggaagctca cccaggactc ctgcctgttg catcagcgcg
     3901 tcttaaaggc tctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     3961 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag
     4021 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
     4081 aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg
     4141 acgaagcgtt gcgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctgggac
     4201 gtcgatgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
     4261 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
     4321 acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccgactgg atcgaaaaac
     4381 gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg
     4441 agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
     4501 tactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
     4561 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga
     4621 tggagcttga agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata
     4681 aagcaggcga ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc
     4741 acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagt
     4801 tggaaccggc ggatgatttt cgtgacgagc cggtgaagtt aacggcgtga gtgaaatgtg
     4861 ccggatgcat cacatccggc aataatcatt aaaactgata ggtcatgcca accgcgacaa
     4921 tatcatcatt attaatattc aatttgttat cgctatccag ttggttgatt ttataatcaa
     4981 caaacgctga catatttttg ttgaaataat atgtagcccc gacatcgata taattgacca
     5041 gatcttcatc accgatacct tcaatatctt tccctttcga taagacataa cccagcgatg
     5101 gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg
     5161 caaagccgcc agttattggc gtcattttgc gtgtttcaga ataaaaagtt gccagataaa
     5221 tattattggc atcgtatttc agacctgtag cccaagcttc tgcacgcttg cctgtgccac
     5281 ggctttgcag gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga
     5341 aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt
     5401 taacgtcgcg gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa
     5461 cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct ggcgcg
//
LOCUS       DAZFDC010000533         4519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.guided.222, whole genome shotgun sequence.
ACCESSION   DAZFDC010000533 DAZFDC010000000
VERSION     DAZFDC010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.guided.222"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..1173
                     /gene="ampC"
                     /locus_tag="ACHJ48_005382"
     CDS             40..1173
                     /gene="ampC"
                     /locus_tag="ACHJ48_005382"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO4710674.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1261..1794
                     /gene="blc"
                     /locus_tag="ACHJ48_005383"
     CDS             1261..1794
                     /gene="blc"
                     /locus_tag="ACHJ48_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO4710675.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1791..2108)
                     /gene="sugE"
                     /locus_tag="ACHJ48_005384"
     CDS             complement(1791..2108)
                     /gene="sugE"
                     /locus_tag="ACHJ48_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO4710676.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2284..2430)
                     /gene="ecnB"
                     /locus_tag="ACHJ48_005385"
     CDS             complement(2284..2430)
                     /gene="ecnB"
                     /locus_tag="ACHJ48_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO4710677.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2541..2666)
                     /gene="ecnA"
                     /locus_tag="ACHJ48_005386"
     CDS             complement(2541..2666)
                     /gene="ecnA"
                     /locus_tag="ACHJ48_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO4710678.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2718..3284)
                     /gene="efp"
                     /locus_tag="ACHJ48_005387"
     CDS             complement(2718..3284)
                     /gene="efp"
                     /locus_tag="ACHJ48_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO4710679.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3326..4354
                     /gene="epmB"
                     /locus_tag="ACHJ48_005388"
     CDS             3326..4354
                     /gene="epmB"
                     /locus_tag="ACHJ48_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO4710680.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1156 a         1148 c         1122 g         1093 t
ORIGIN      
        1 ccgcctatgg cgggccgttt tgtatggaaa ccagacccta tgttcaaaac gacgctctgc
       61 gccttattaa ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat caacgatatt
      121 gtgcatcgca caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg
      181 gtaatttatc agggtaaacc ttattacttt acctgggggt atgcggacat cgccaaaaag
      241 cagcccgtca cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc
      301 gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa
      361 tactggcctg aacttaccgc taaacagtgg aatgggatca cactattaca tctcgcaacc
      421 tacactgctg gcggcctgcc attgcaggtg ccggatgaag tgaaatcctc aagcgacttg
      481 ctgcgcttct atcaaaactg gcagcctgca tgggctccag gaacacaacg tctgtatgcc
      541 aactccagta tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag
      601 caggcgatgc aaactcgtgt cttccagcca ctcaaactca accatacgtg gattaatgta
      661 ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg aaggcaaggc agttcatgtt
      721 tcgccagggg cgttagatgc tgaaacttat ggtgtgaagt cgaccattga agatatggcc
      781 tgctgggtac gaagcaatat gaatccccgt gatatcaacg acaaaacact tcagcaaggg
      841 atacaactgg cacaatctcg ctactggcaa accggcgata tgtatcaggg tctgggctgg
      901 gaaatgctgg actggccggt aaatcctgac atcatcgtta acggcagtga caataaaatt
      961 gcactggcag cacgccccgt aaaagcgatt acgcctccaa ctcctgcagt acgagcatca
     1021 tgggtgcata aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa
     1081 aaagagctgg gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc
     1141 gccgcctggc agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac
     1201 cttttctgcg cttttccttg ctgtcatcta cacttagaaa aaaccagtaa ggaaacattt
     1261 atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt
     1321 tctcctacgc cgccgcgtgg cgtgaccgta gtgaataatt tcgacgctaa acgctatctc
     1381 ggtacctggt atgagattgc ccgttttgat caccgctttg aacgtggact ggaaaaagtc
     1441 accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac
     1501 cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact
     1561 cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca
     1621 ctcgatcggg aataccgcca tgcgctggtt tgcggaccgg accgcgacta cctgtggata
     1681 ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc
     1741 cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagtgagtg
     1801 ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag acgcatcggg
     1861 ttagcggact caccgagcag cacaatgccg gtgatggccg cgccgactgc gccaataccc
     1921 gtccacacgg cataagccgt ccctactggt aacgatttca tcgcccaggc aagtagcgcc
     1981 atactgacaa tcatcgccgt cacggtaata acactcggcg tcaaacgact aaagccgtgg
     2041 gtatatttca ggcctacggc ccataccact tccagcagac cagcaataac taagataatc
     2101 caggacatat caggctccgg agaattgggg ccgtccccgg tgaaaagacg cgtttgcagg
     2161 tcgtcctgca aagctgatgg ttgaacggat attttgccag gtggcggggg aatttcaact
     2221 attacctttg tgactatcgc agaaaaaaca aacggcacga cacagctgtc gtaccgttat
     2281 tgcttattgc tgcgctttcg ttgctgcacc agaaatcgcg ttaccgccat cagaaatgtc
     2341 ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact gttgaaagca ccagaacaga
     2401 aaagatcgct gcaattgtct tcttcaccat aacgtcttcc ttttaggtaa tgttatttat
     2461 gtttgcctat agcaaattaa taatagacaa catcgccaaa aatgatggaa atctgatgat
     2521 ttttcggaag agaagaaaaa ttagctggca gcgcgggaga tggagttgcc gagatgtttg
     2581 atgtcttcgc cgaaaccgcg agcggtgtta cagcccgtga gcagcgtgct ggcaagcaaa
     2641 acaagaacga taaggcgttt catcatctct gacctgaata agtgatggtg cagcctgcag
     2701 gccgcaccac aaccgcatta cttcacgcga gagacgtatt caccagagcg ggtatccact
     2761 ttgatgactt cgccgatttg tacgaacagc ggaactttaa ccacagcgcc agtagacagg
     2821 gtagccggtt tgccgccagt acctgcggta tcacctttca ggcccggatc ggtatcaacg
     2881 atttccagtt caacgaagtt cggcggagta acggagatcg gctgaccatt ccacagagtt
     2941 acgatacact ctgcctgatc cagcagccat ttagcgttgt cgccaattgc ttttgcgtca
     3001 gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc agaactcacc gtcgttgtac
     3061 aggtaagtca ggttcatatc gacaacatca gcgccttcag cggaatcagt agatttgaag
     3121 gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt taacgcgagc aaatgcctgg
     3181 cctttacccg gttttacgaa ttcactcgct tcaaccgcgt aaggttcgcc gtctaacatg
     3241 attttaagac cagcacgaaa atcgttgcta tagtacgttg ccataaggcc ctctgaaatt
     3301 tgttaattgg tagctaagcc acaaaatggc gcatattgta accctaaata ccccatccag
     3361 agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat cctgatgaac ttctgcgtct
     3421 tttgaatata gacgcggacg aaaaactgtt ggccggacgc aacgccaaaa agctgtttgc
     3481 cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa ggcaatccgg acgatccgct
     3541 tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc gcgcccggat tctccaccga
     3601 cccgctggaa gaacagcaca gcgtagtgcc tggtttgttg cataaatacc acaaccgggc
     3661 gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc tattgcttcc gtcgccactt
     3721 cccctatgcc gaaaatcagg gcaacaagcg taactggcaa actgcacttg agtatgttgc
     3781 tgcgcatccg gaactggacg agatgatttt ctccggcggc gatccgctga tggcgaaaga
     3841 tcacgagctg gactggttgc tcacacaact ggaagccatc ccgcatatca aacgtctgcg
     3901 gattcacagt cgtctgccga ttgtgatccc ggcacgcatc acagatgcgc tggttgaacg
     3961 cttttcacat tctacgctgc aaatcttgct ggtgaatcac atcaaccatg ccaatgagat
     4021 agatgaaaca ttccgtcagg cgatggctaa gttgcgccgg gcgggcgtta ctttgctgaa
     4081 ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa acgctggcaa acctgagtaa
     4141 tgcgttgttc gatgcaggcg tgatgccgta ttacctgcat gtgctcgata aagtacaggg
     4201 cgcggcgcat tttatggtga gtgatgacga agcacggcag attatgcgtg agttgctgac
     4261 actggtgtcg ggatatctgg tgccgaaact ggcgcgagaa attggcggcg aacccagcaa
     4321 aacgccgctg gatctccagc tacgccagca gtaactgatt aaacagggaa gtttaatctt
     4381 tgcccgttac ataaccttta attattcata tacattgctg ttgttactct ttatataacc
     4441 aacaccaata attaaccttt cttttctgtt tatatagagt gagtacggct atttatgtta
     4501 ttttaacaac tattactac
//
LOCUS       DAZFDC010000534         1497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2205T73291
            SAMN43267900-rid23064823.guided.336, whole genome shotgun sequence.
ACCESSION   DAZFDC010000534 DAZFDC010000000
VERSION     DAZFDC010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267900
            Sequence Read Archive: SRR30308020
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351336.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351336.1
            Genome Coverage       :: 108x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:00:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,390
            CDSs (total)                      :: 5,296
            Genes (coding)                    :: 5,040
            CDSs (with protein)               :: 5,040
            Genes (RNA)                       :: 94
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 107 of 256
            Pseudo Genes (incomplete)         :: 138 of 256
            Pseudo Genes (internal stop)      :: 61 of 256
            Pseudo Genes (multiple problems)  :: 40 of 256
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267900-rid23064823.guided.336"
                     /serotype="O157:H 7"
                     /isolate="2205T73291"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            131..1090
                     /locus_tag="ACHJ48_005389"
     CDS             131..1090
                     /locus_tag="ACHJ48_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO4710681.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1102..1371
                     /locus_tag="ACHJ48_005390"
     CDS             1102..1371
                     /locus_tag="ACHJ48_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO4710682.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          405 a          300 c          365 g          427 t
ORIGIN      
        1 aagcaatcgg tcactggttc gaatccagta caacgcgcca tatttattta ccaggctcgc
       61 ttttgcgggc cttttttata tctgcgccgg gtctggtgct gattacttca gccaaaagga
      121 acacctgtat atgaagtgta tattatttaa atgggtactg tgcctgttac tgggtttttc
      181 ttcggtatcc tattcccggg aatttacgat agacttttcg actcaacaaa gttatgtctc
      241 ttcgttaaat agtatacgga cagagatatc gacccctctt gaacatatat ctcaggggac
      301 cacatcggtg tctgttatta accacacccc gccgggcagt tattttgctg tggatatacg
      361 agggcttgat gtctatcagg cgcgttttga ccatcttcgt ctgattattg agcaaaataa
      421 tttatatgtg gccgggttcg ttaatacggc aacaaatact ttctaccgtt tttcagattt
      481 tacacatata tcagtgcccg atgtgacaac ggtttccatg acaacggaca gcagttatac
      541 cactctgcaa cgtgtcgcag cgctggaacg ttccggaatg caaatcagtc gtcactcact
      601 ggtttcatca tatctggcgt taatggagtt cagtggtaat acaatgacca gagatgcatc
      661 cagagcagtt ctgcgttttg tcactgtcac ggcagaagcc ttacgcttca ggcagataca
      721 gagagaattt cgtcaggcac tgtctgaaac tgctcctgtg tatacgatga cgccgggaga
      781 cgtggacctc actctgaact gggggcgaat cagcaatgtg cttccggagt atcggggaga
      841 ggatggtgtc agagtgggga gaatatcctt taataatata tcagcgatac tgggtactgt
      901 ggccgttata ctgaattgcc atcatcaggg ggcgcgttct gttcgcgccg tgaatgaaga
      961 gagtcaacca gaatgtcaga taactggcga caggcccgtt ataaaaataa acaatacatt
     1021 atgggaaagt aatacagctg cagcgtttct gaacagaaag tcacagtttt tatatacaac
     1081 gggtaaataa aggagttaag tatgaagaag atgtttatgg cggttttatt tgcattagct
     1141 tctgttaatg caatggcggc ggattgtgct aaaggtaaaa ttgagttttc caagtataat
     1201 gaggatgaca catttacagt gaaggttgac gggaaagaat actggaccag tcgctggaat
     1261 ctgcaaccgt tactgcaaag tgctcagttg acaggaatga ctgtcacaat caaatccagt
     1321 acctgtgaat caggctccgg atttgctgaa gtgcagttta ataatgactg aggcataacc
     1381 tgattcgtgg tatgtgggta acaagtgtaa tctgtgtcac aattcagtca gttgacagtt
     1441 gcctgtcaga ctgagcattt gttaaaaaaa tttcgcatgg tgaatccccc tgtgcgg
//
